title: Unique structural properties associated with mouse prion Δ105-125 protein.
abstract: Murine prion protein deleted for residues 105-125 is intrinsically neurotoxic and mediates a TSE-like phenotype in transgenic mice. Equivalent and overlapping deletions were expressed in E.coli, purified and analyzed. Among mutants spanning the region 95-135, a construct lacking solely residues 105-125 had distinct properties when compared with the full-length prion protein 23-231 or other deletions. This distinction was also apparent followed expression in eukaryotic cells. Unlike the full-length protein, all deletion mutants failed to bind to synthetic membranes in vitro. These data suggest a novel structure for the 105-125 deleted variant that may relate to its biological properties.
authors.affiliation: School of Biological Sciences, University of Reading, Reading, United Kingdom.
authors.forename: Avnish
authors.initials: A
authors.lastname: Patel
authors.forename: Snezana
authors.initials: S
authors.lastname: Vasiljevic
authors.forename: Ian M
authors.initials: IM
authors.lastname: Jones
mesh_terms: D000818:Animals; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D015870:Gene Expression; D008081:Liposomes / Q000378:metabolism; D051379:Mice; D010715:Phosphatidylglycerols / Q000378:metabolism; D000072002:Prion Proteins; D011328:Prions / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D011485:Protein Binding; D017433:Protein Structure, Secondary; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D017384:Sequence Deletion
chemical_list: D008081:Liposomes; D010715:Phosphatidylglycerols; D000072002:Prion Proteins; D011328:Prions; C512657:Prnp protein, mouse; D011993:Recombinant Fusion Proteins; C060037:1-palmitoyl-2-oleoylglycero-3-phosphoglycerol
keywords: E.coli; baculovirus; deletion; expression; folding; hydrophobic; murine PrPC; toxicity