title: Near-infrared fluorescent proteins for multicolor in vivo imaging.
abstract: Near-infrared fluorescent proteins (FPs) are in high demand for in vivo imaging. We developed four spectrally distinct near-infrared FPs--iRFP670, iRFP682, iRFP702 and iRFP720--from bacterial phytochromes. iRFPs exhibit high brightness in mammalian cells and tissues and are suitable for long-term studies. iRFP670 and iRFP720 enable two-color imaging with standard approaches in living cells and mice. The four new iRFPs and the previously engineered iRFP713 allow multicolor imaging with spectral unmixing in living mice.
authors.affiliation: Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, Bronx, New York, USA.
authors.forename: Daria M
authors.initials: DM
authors.lastname: Shcherbakova
authors.forename: Vladislav V
authors.initials: VV
authors.lastname: Verkhusha
mesh_terms: D000818:Animals; D001483:Base Sequence; D003952:Diagnostic Imaging / Q000379:methods*; D005260:Female; D006367:HeLa Cells; D006801:Humans; D008164:Luminescent Proteins / Q000737:chemistry*; D051379:Mice; D016513:Mice, SCID; D008856:Microscopy, Fluorescence / Q000379:methods*; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D009374:Neoplasms, Experimental / Q000175:diagnosis; D010834:Phytochrome / Q000737:chemistry*; D017422:Sequence Analysis, DNA
chemical_list: D008164:Luminescent Proteins; D010834:Phytochrome