title: Membrane proteins of Pseudoalteromonas tunicata during the transition from planktonic to extracellular matrix-adherent state.
abstract: Pseudoalteromonas tunicata is a marine bacterium that was originally isolated from the surface of the tunicate Ciona intestinalis. Since C. intestinalis expresses extracellular matrix (ECM) and P. tunicata has a gene encoding a functional ECM-binding protein, we hypothesized that P. tunicata could adhere to this host via protein-ECM interactions and as a result change its membrane proteome. An in vitro adhesion assay was developed to show that P. tunicata adheres strongly to ECM. To further study the adhesion biology of P. tunicata, two-dimensional (2D) electrophoresis was used to explore the membrane-associated sub-proteome of P. tunicata during planktonic, adherent and non-adherent states. More than 30 proteins were resolved using blue native (BN)/SDS 2D PAGE, many of which were identified by mass spectrometry. BN/SDS PAGE also allowed the identification of several novel protein complexes, which indicate structural and functional relationships for these proteins and related proteins in several other organisms. A proteomic change associated with adhesion was identified by comparison of 2D gels from the three model states. Collectively, these studies explore the membrane proteome of P. tunicata during the transition from planktonic to ECM-adherent states.
authors.affiliation: Departments of Biochemistry and Molecular Biology Microbiology, Monash University, Clayton, Vic. 3800, Australia Australian Research Council Centre of Excellence in Structural and Functional Microbial Genomics, Monash University, Clayton, Vic. 3800, Australia Centre for Marine Bio-Innovation and School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, NSW 2052, Australia.
authors.forename: David E
authors.initials: DE
authors.lastname: Hoke
authors.forename: Kunkun
authors.initials: K
authors.lastname: Zhang
authors.forename: Suhelen
authors.initials: S
authors.lastname: Egan
authors.forename: Tamas
authors.initials: T
authors.lastname: Hatfaludi
authors.forename: Ashley M
authors.initials: AM
authors.lastname: Buckle
authors.forename: Ben
authors.initials: B
authors.lastname: Adler