title: Functional recovery and alterations in the expression and localization of protein kinase C following voluntary exercise in rat with cerebral infarction.
abstract: Recently, it has become widely known that rehabilitative training after stroke brings about some improvement of paralysis and disability; however, not much is known about the relationship between paralysis recovery and the participation of plasticity-related molecules. Hence, the localization and level of expression of several proteins in the cerebral cortex of rat groups with/without voluntary exercise using a running wheel after photo thrombotic infarction were examined in this study. In behavioral evaluation, the mean latency until falling from a rotating rod in the group with voluntary exercise at 6 days after infarction was significantly longer than that in the group without exercise. Immunohistochemical localization of c-Fos protein after behavioral test occurred in the area surrounding the infarction core in the exercise group. In protein expression analysis, protein kinase C (PKC), growth-associated protein 43 (GAP43) and phosphorylated at serine 41 GAP43 (p-GAP43) were significantly increased after voluntary exercise compared with those in rats without exercise. Expression of PKC immunoreactivity was observed in layer III of the perilesional cortex in rats with exercise, and the intracellular localization in the pyramidal neurons was mainly translocated to the plasma membrane. The expression and localization of these proteins may be related to the underlying mechanisms of exercise-induced paralysis recovery, that is, neuronal plasticity and remodeling of cortical connections through the phosphorylation of GAP43 by interaction with PKC. In the present study, the participation of at least some of the modulators associated with the improvement of motor deficit adjacent to the brain lesion might have been detected.
authors.affiliation: Division of Biochemistry, Fujita Memorial Nanakuri Institute, Fujita Health University, 1865 Hisai-isshiki-cho, Tsu, Mie, 514-1296, Japan, kenmei@fujita-hu.ac.jp.
authors.forename: Kenmei
authors.initials: K
authors.lastname: Mizutani
authors.forename: Shigeru
authors.initials: S
authors.lastname: Sonoda
authors.forename: Hideaki
authors.initials: H
authors.lastname: Wakita
authors.forename: Yoshimitsu
authors.initials: Y
authors.lastname: Katoh
authors.forename: Kan
authors.initials: K
authors.lastname: Shimpo
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002540:Cerebral Cortex / Q000201:enzymology; D002544:Cerebral Infarction / Q000201:enzymology* / Q000534:rehabilitation*; D007150:Immunohistochemistry; D008297:Male; D010805:Physical Conditioning, Animal; D011493:Protein Kinase C / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D020127:Recovery of Function / Q000502:physiology*
chemical_list: D011493:Protein Kinase C