title: Investigation of the esophageal Rho-kinase expression in patients with Barrett's esophagus.
abstract: The mechanisms responsible for the malignant transformation in Barrett's esophagus (BE) are still poorly understood. The authors have evaluated the role of Rho-kinase (ROCK1 and ROCK2) expressions in patients with BE. All patients underwent upper gastrointestinal system endoscopy, which was confirmed histologically. Real-time PCR revealed no marked change in gene expressions of ROCK1 and ROCK2 at mRNA levels in BE when compared to controls. Immunohistochemical and western blot analyses showed no change in ROCK1 and ROCK2 protein expressions in BE. This study demonstrates that Rho-kinase gene and protein expressions are not modified in BE.
authors.affiliation: Department of Physiology, Faculty of Medicine, University of Gaziantep, Gaziantep, Turkey.
authors.forename: Seniz
authors.initials: S
authors.lastname: Demiryürek
authors.forename: Irfan
authors.initials: I
authors.lastname: Koruk
authors.forename: Zehra
authors.initials: Z
authors.lastname: Bozdag
authors.forename: Esma
authors.initials: E
authors.lastname: Ozkara
authors.forename: Davut Sinan
authors.initials: DS
authors.lastname: Kaplan
authors.forename: Serdar
authors.initials: S
authors.lastname: Oztuzcu
authors.forename: Ali
authors.initials: A
authors.lastname: Cetinkaya
authors.forename: Belgin
authors.initials: B
authors.lastname: Alasehirli
authors.forename: Abdullah Tuncay
authors.initials: AT
authors.lastname: Demiryürek
mesh_terms: D001471:Barrett Esophagus / Q000201:enzymology*; D015153:Blotting, Western; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D054460:rho-Associated Kinases / Q000032:analysis / Q000096:biosynthesis*
chemical_list: C515851:ROCK1 protein, human; C515852:ROCK2 protein, human; D054460:rho-Associated Kinases