title: Serum amyloid A is found on ApoB-containing lipoproteins in obese humans with diabetes.
abstract: OBJECTIVE
  In murine models of obesity/diabetes, there is an increase in plasma serum amyloid A (SAA) levels along with redistribution of SAA from high-density lipoprotein (HDL) to apolipoprotein B (apoB)-containing lipoprotein particles, namely, low-density lipoprotein and very low-density lipoprotein. The goal of this study was to determine if obesity is associated with similar SAA lipoprotein redistribution in humans.
  
  
  METHODS
  Three groups of obese individuals were recruited from a weight loss clinic: healthy obese (n = 14), metabolic syndrome (MetS) obese (n = 8), and obese with type 2 diabetes (n = 6). Plasma was separated into lipoprotein fractions by fast protein liquid chromatography, and SAA was measured in lipid fractions using enzyme-linked immunosorbent assay and Western blotting.
  
  
  RESULTS
  Only the obese diabetic group had SAA detectable in apoB-containing lipoproteins, and SAA reverted back to HDL with active weight loss.
  
  
  CONCLUSIONS
  In human subjects, SAA is found in apoB-containing lipoprotein particles only in obese subjects with type 2 diabetes, but not in healthy obese or obese subjects with MetS.
authors.affiliation: Division of Endocrinology and Molecular Medicine, University of Kentucky, Lexington, Kentucky, USA.
authors.forename: Anisa
authors.initials: A
authors.lastname: Jahangiri
authors.forename: Patricia G
authors.initials: PG
authors.lastname: Wilson
authors.forename: Tianfei
authors.initials: T
authors.lastname: Hou
authors.forename: Aparna
authors.initials: A
authors.lastname: Brown
authors.forename: Victoria L
authors.initials: VL
authors.lastname: King
authors.forename: Lisa R
authors.initials: LR
authors.lastname: Tannock
mesh_terms: D001055:Apolipoproteins B / Q000097:blood*; D003924:Diabetes Mellitus, Type 2 / Q000097:blood* / Q000150:complications; D006801:Humans; D008074:Lipoproteins / Q000097:blood*; D008075:Lipoproteins, HDL / Q000097:blood; D008077:Lipoproteins, LDL / Q000097:blood; D008079:Lipoproteins, VLDL / Q000097:blood; D024821:Metabolic Syndrome / Q000097:blood* / Q000150:complications; D009765:Obesity / Q000097:blood* / Q000150:complications; D000685:Serum Amyloid A Protein / Q000378:metabolism*; D015431:Weight Loss
chemical_list: D001055:Apolipoproteins B; D008074:Lipoproteins; D008075:Lipoproteins, HDL; D008077:Lipoproteins, LDL; D008079:Lipoproteins, VLDL; D000685:Serum Amyloid A Protein