title: Isolation and characterization of a Myoviridae bacteriophage against Staphylococcus aureus isolated from dairy cows with mastitis.
abstract: A lytic bacteriophage (phage), designated SAH-1, was isolated from sewage effluent near a dairy cow farm in Gwacheon, South Korea to search for biocontrol agents against Staphylococcus aureus infections. The SAH-1 was morphologically classified as Myoviridae and possessed an approximate 144 kb double-stranded genomic DNA. The phage showed broad host ranges within S. aureus strains including methicillin-resistant strains, and its latent period and burst size were approximately 20 min and 100 PFU/cell, respectively. Moreover, morphologic and genomic analysis of SAH-1 revealed that the phage was closely related to other Myoviridae phages infecting Staphylococcus species. The bacteriolytic activity of phage SAH-1 at a multiplicity of infection (MOI) 1 and 100 indicated its efficiency for reducing bacterial growth. Based on these results, phage SAH-1 could be considered a potential therapeutic or prophylactic candidate against S. aureus infections.
authors.affiliation: Laboratory of Aquatic Biomedicine, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul, Republic of Korea.
authors.forename: Jee Eun
authors.initials: JE
authors.lastname: Han
authors.forename: Ji Hyung
authors.initials: JH
authors.lastname: Kim
authors.forename: Sun Young
authors.initials: SY
authors.lastname: Hwang
authors.forename: Casiano H
authors.initials: CH
authors.lastname: Choresca
authors.forename: Sang Phil
authors.initials: SP
authors.lastname: Shin
authors.forename: Jin Woo
authors.initials: JW
authors.lastname: Jun
authors.forename: Ji Young
authors.initials: JY
authors.lastname: Chai
authors.forename: Yong Ho
authors.initials: YH
authors.lastname: Park
authors.forename: Se Chang
authors.initials: SC
authors.lastname: Park
mesh_terms: D000818:Animals; D002417:Cattle; D005260:Female; D008414:Mastitis, Bovine / Q000382:microbiology*; D017900:Myoviridae / Q000145:classification* / Q000302:isolation & purification*; D013203:Staphylococcal Infections / Q000382:microbiology / Q000662:veterinary*; D013211:Staphylococcus aureus / Q000302:isolation & purification / Q000821:virology*
keywords: Bacteriophage; Mastitis; Myoviridae; SAH-1; Staphylococcus aureus