title: Antimicrobial efficacy of a novel silver hydrogel dressing compared to two common silver burn wound dressings: Acticoat™ and PolyMem Silver(®).
abstract: A novel burn wound hydrogel dressing has been previously developed which is composed of 2-acrylamido-2-methylpropane sulfonic acid sodium salt with silver nanoparticles. This study compared the antimicrobial efficacy of this novel dressing to two commercially available silver dressings; Acticoat™ and PolyMem Silver(®). Three different antimicrobial tests were used: disc diffusion, broth culture, and the Live/Dead(®) Baclight™ bacterial viability assay. Burn wound pathogens (P. aeruginosa, MSSA, A. baumannii and C. albicans) and antibiotic resistant strains (MRSA and VRE) were tested. All three antimicrobial tests indicated that Acticoat™ was the most effective antimicrobial agent, with inhibition zone lengths of 13.9-18.4mm. It reduced the microbial inocula below the limit of detection (10(2)CFU/ml) and reduced viability by 99% within 4h. PolyMem Silver(®) had no zone of inhibition for most tested micro-organisms, and it also showed poor antimicrobial activity in the broth culture and Live/Dead(®) Baclight™ assays. Alarmingly, it appeared to promote the growth of VRE. The silver hydrogel reduced most of the tested microbial inocula below the detection limit and decreased bacterial viability by 94-99% after 24h exposure. These results support the possibility of using this novel silver hydrogel as a burn wound dressing in the future.
authors.affiliation: Centre for Children's Burns and Trauma Research, Queensland Children's Medical Research Institute, The University of Queensland, Brisbane, Queensland, Australia; The Petroleum and Petrochemical College, Chulalongkorn University, Bangkok, Thailand.
authors.forename: Benjawan
authors.initials: B
authors.lastname: Boonkaew
authors.affiliation: Centre for Children's Burns and Trauma Research, Queensland Children's Medical Research Institute, The University of Queensland, Brisbane, Queensland, Australia.
authors.forename: Margit
authors.initials: M
authors.lastname: Kempf
authors.affiliation: Centre for Children's Burns and Trauma Research, Queensland Children's Medical Research Institute, The University of Queensland, Brisbane, Queensland, Australia.
authors.forename: Roy
authors.initials: R
authors.lastname: Kimble
authors.affiliation: The Petroleum and Petrochemical College, Chulalongkorn University, Bangkok, Thailand.
authors.forename: Pitt
authors.initials: P
authors.lastname: Supaphol
authors.affiliation: Centre for Children's Burns and Trauma Research, Queensland Children's Medical Research Institute, The University of Queensland, Brisbane, Queensland, Australia. Electronic address: L.Cuttle@uq.edu.au.
authors.forename: Leila
authors.initials: L
authors.lastname: Cuttle
mesh_terms: D040981:Acinetobacter baumannii / Q000187:drug effects*; D000178:Acrylamides / Q000494:pharmacology*; D000476:Alkanesulfonates / Q000494:pharmacology*; D000891:Anti-Infective Agents, Local / Q000494:pharmacology*; D047070:Bandages, Hydrocolloid; D002056:Burns / Q000628:therapy; D002176:Candida albicans / Q000187:drug effects*; D016983:Enterococcus / Q000187:drug effects*; D005990:Glycerol / Q000494:pharmacology; D006801:Humans; D053768:Metal Nanoparticles; D055624:Methicillin-Resistant Staphylococcus aureus / Q000187:drug effects*; D008826:Microbial Sensitivity Tests; D011091:Polyesters / Q000494:pharmacology; D011095:Polyethylenes / Q000494:pharmacology; D011140:Polyurethanes / Q000494:pharmacology; D011550:Pseudomonas aeruginosa / Q000187:drug effects*; D012834:Silver / Q000494:pharmacology*; D013211:Staphylococcus aureus / Q000187:drug effects
chemical_list: D000178:Acrylamides; C117408:Acticoat; D000476:Alkanesulfonates; D000891:Anti-Infective Agents, Local; D011091:Polyesters; D011095:Polyethylenes; C088317:Polymem; D011140:Polyurethanes; D012834:Silver; C056288:2-acrylamido-2-methylpropanesulfonate; D005990:Glycerol
keywords: Antimicrobial; Broth culture; Disc diffusion; Dressing; Live/Dead; Silver