title: Exacerbation of experimental autoimmune encephalomyelitis by passive transfer of IgG antibodies from a multiple sclerosis patient responsive to immunoadsorption.
abstract: The pathogenic role of antibodies in multiple sclerosis (MS) is still controversial. We transferred to mice with experimental autoimmune encephalomyelitis (EAE), animal model of MS, IgG antibodies purified from a MS patient presenting a dramatic clinical improvement during relapse after selective IgG removal with immunoadsorption. Passive transfer of patient's IgG exacerbated motor paralysis and increased mouse central nervous system (CNS) inflammation and demyelination. Binding of patient's IgG was demonstrated in mouse CNS, with a diffuse staining of white matter oligodendrocytes. These data support a growing body of evidence that antibodies can play an important role in the pathobiology of MS.
authors.affiliation: Neuroimmunology and Neuromuscular Disorders Unit, Foundation IRCCS Neurological Institute C. Besta, Via Celoria 11, 20133 Milan, Italy. Electronic address: rpedotti@istituto-besta.it.
authors.forename: Rosetta
authors.initials: R
authors.lastname: Pedotti
authors.forename: Silvia
authors.initials: S
authors.lastname: Musio
authors.forename: Stefano
authors.initials: S
authors.lastname: Scabeni
authors.forename: Cinthia
authors.initials: C
authors.lastname: Farina
authors.forename: Pietro Luigi
authors.initials: PL
authors.lastname: Poliani
authors.forename: Emanuela
authors.initials: E
authors.lastname: Colombo
authors.forename: Massimo
authors.initials: M
authors.lastname: Costanza
authors.forename: Angela
authors.initials: A
authors.lastname: Berzi
authors.forename: Fabrizio
authors.initials: F
authors.lastname: Castellucci
authors.forename: Emilio
authors.initials: E
authors.lastname: Ciusani
authors.forename: Paolo
authors.initials: P
authors.lastname: Confalonieri
authors.forename: Bernhard
authors.initials: B
authors.lastname: Hemmer
authors.forename: Renato
authors.initials: R
authors.lastname: Mantegazza
authors.forename: Carlo
authors.initials: C
authors.lastname: Antozzi
mesh_terms: D000328:Adult; D000818:Animals; D002490:Central Nervous System / Q000187:drug effects*; D003711:Demyelinating Diseases / Q000139:chemically induced; D004195:Disease Models, Animal; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000276:immunology* / Q000473:pathology*; D005260:Female; D006801:Humans; D007116:Immunization, Passive / Q000379:methods; D007074:Immunoglobulin G / Q000008:administration & dosage*; D007163:Immunosorbent Techniques; D007164:Immunosorbents / Q000008:administration & dosage*; D051379:Mice; D020529:Multiple Sclerosis, Relapsing-Remitting / Q000276:immunology*; D010951:Plasma Exchange / Q000379:methods
chemical_list: D007074:Immunoglobulin G; D007164:Immunosorbents
keywords: Antibodies; Demyelination; EAE; Experimental autoimmune encephalomyelitis; Immunoadsorption; LNCs; MS; Multiple sclerosis; PBS; PLP; Plasma exchange; T helper; TPE; Th; experimental autoimmune encephalomyelitis; lymph node cells; multiple sclerosis; myelin proteolipid protein; phosphate buffer saline; plasma exchange