title: Tissue-engineered heart valves: intra-operative protocol.
abstract: Tissue engineering of heart valves investigates the possibility to create a fully compatible and biomimetic graft able to provide host cell repopulation like the native living valve. Decellularized aortic and pulmonary valves and synthetic polymers have been used to promote the creation of a native-like scaffold suitable to be colonized by cells either in vitro, in dynamic bioreactors or in vivo using different animal models. The herein presented research provides the intra-operative protocol and details of surgical technique. Porcine aortic valve conduits were decellularized and implanted in the right ventricular outflow tract of Vietnamese pigs.
authors.affiliation: Division of Cardiac Surgery, Department of Cardiac, Thoracic and Vascular Science, University of Padua, Via Giustiniani 2, 35128, Padua, Italy. michelegallo@hotmail.co.uk
authors.forename: Michele
authors.initials: M
authors.lastname: Gallo
authors.forename: Roberto
authors.initials: R
authors.lastname: Bianco
authors.forename: Tomaso
authors.initials: T
authors.lastname: Bottio
authors.forename: Filippo
authors.initials: F
authors.lastname: Naso
authors.forename: Paolo
authors.initials: P
authors.lastname: Franci
authors.forename: Fabio
authors.initials: F
authors.lastname: Zanella
authors.forename: Giovanni
authors.initials: G
authors.lastname: Perona
authors.forename: Roberto
authors.initials: R
authors.lastname: Busetto
authors.forename: Michele
authors.initials: M
authors.lastname: Spina
authors.forename: Alessandro
authors.initials: A
authors.lastname: Gandaglia
authors.forename: Gino
authors.initials: G
authors.lastname: Gerosa
mesh_terms: D000818:Animals; D001021:Aortic Valve / Q000601:surgery*; D001705:Bioprosthesis; D019149:Bioreactors; D002478:Cells, Cultured; D006350:Heart Valve Prosthesis; D019918:Heart Valve Prosthesis Implantation / Q000295:instrumentation*; D023421:Models, Animal; D011474:Prosthesis Design; D011664:Pulmonary Valve / Q000601:surgery*; D013552:Swine; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds