title: Robust size control of bovine serum albumin (BSA) nanoparticles by intermittent addition of a desolvating agent and the particle formation mechanism.
abstract: In polymeric nanoparticle preparation, despite similar conditions, large fluctuations in particle size distributions are usually observed. Herein, we demonstrate that the intermittent addition of a desolvating agent can improve reproducibility in the preparation of polymeric bovine serum albumin (BSA) nanoparticles. Using this modification, BSA nanoparticles of controlled size can be manufactured with narrow particle size distributions. In our study, ethanol as a desolvating agent was added intermittently to 1% BSA solutions at different pHs with stirring at 700rpm. The effect of the preparation parameters on size and optical density of the fabricated nanoparticles were studied. The average particles sizes of BSA nanoparticles prepared at pH values of 6, 7 and 9 were approximately 100, 200 and 300nm, respectively. As ethanol addition increased, desolvation of BSA molecules resulted in formation of loose-structured particles with pH-dependent size. Beyond that, only particle density increased, but size remained unchanged with further addition of ethanol. Consistently uniform particle size distribution was achieved by adding ethanol intermittently.
authors.affiliation: Department of Food Science and Technology, Sejong University, 98 Gunja-dong, Gwangjin-gu, Seoul 143-747, Republic of Korea. serim0506@naver.com
authors.forename: Sae-Yeol-Rim
authors.initials: SY
authors.lastname: Paik
authors.forename: Hoang Hai
authors.initials: HH
authors.lastname: Nguyen
authors.forename: Jina
authors.initials: J
authors.lastname: Ryu
authors.forename: Jeong-Hwan
authors.initials: JH
authors.lastname: Che
authors.forename: Tae Seok
authors.initials: TS
authors.lastname: Kang
authors.forename: Jong Kwon
authors.initials: JK
authors.lastname: Lee
authors.forename: Chi Won
authors.initials: CW
authors.lastname: Song
authors.forename: Sanghoon
authors.initials: S
authors.lastname: Ko
mesh_terms: D000431:Ethanol / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D058105:Polymerization; D012710:Serum Albumin, Bovine / Q000737:chemistry* / Q000648:ultrastructure
chemical_list: D012710:Serum Albumin, Bovine; D000431:Ethanol