title: Accurate detection of methylated cytosine in complex methylation landscapes.
abstract: Monitoring DNA methylation can be a useful biomarker for disease diagnosis and prognosis. However, monitoring the methylation status of a specific cytosine biomarker is often confounded by heterogeneous peripheral DNA methylation. To address this issue, molecular inversion probes were designed with inosine strategically positioned to complement suspected DNA methylation sites. This enabled the methylation status of a specific cytosine to be accurately measured with a high level of specificity, irrespective of adjacent epigenetic modifications.
authors.affiliation: Centre for Biomarker Research and Development, Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, Brisbane, QLD 4072, Australia.
authors.forename: Ramkumar
authors.initials: R
authors.lastname: Palanisamy
authors.forename: Ashley R
authors.initials: AR
authors.lastname: Connolly
authors.forename: Matt
authors.initials: M
authors.lastname: Trau
mesh_terms: D003596:Cytosine / Q000032:analysis* / Q000378:metabolism; D019175:DNA Methylation / Q000502:physiology; D008745:Methylation; D015336:Molecular Probe Techniques
chemical_list: D003596:Cytosine