title: Double-antibody based immunoassay for the detection of β-casein in bovine milk samples.
abstract: The concentration of casein (CN) is one of the most important parameters for measuring the quality of bovine milk. Traditional approach to CN concentration determination is Kjeldahl, which is an indirect method for determination of total nitrogen content. Here, we described a double-antibody based direct immunoassay for the detection of β-CN in bovine milk samples. Monoclonal antibody (McAb) was used as capture antibody and polyclonal antibody (PcAb) labelled with horseradish peroxidase (HRP) as detection antibody. With the direct immunoassay format, the linear range of the detection was 0.1-10.0 μg mL(-1). The detection limit was 0.04 μg mL(-1). In addition, the concentration of β-CN in real bovine milk samples has been detected by the developed immunoassay. There was a good correlation between the results obtained by the developed technique and Kjeldahl method from commercial samples. Compared to the traditional approach, the advantage of the assay is no need of time-consuming sample pretreatment.
authors.affiliation: Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, Jilin University, Changchun 130062, PR China. zhouyu69@sina.com
authors.forename: Y
authors.initials: Y
authors.lastname: Zhou
authors.forename: F
authors.initials: F
authors.lastname: Song
authors.forename: Y S
authors.initials: YS
authors.lastname: Li
authors.forename: J Q
authors.initials: JQ
authors.lastname: Liu
authors.forename: S Y
authors.initials: SY
authors.lastname: Lu
authors.forename: H L
authors.initials: HL
authors.lastname: Ren
authors.forename: Z S
authors.initials: ZS
authors.lastname: Liu
authors.forename: Y Y
authors.initials: YY
authors.lastname: Zhang
authors.forename: L
authors.initials: L
authors.lastname: Yang
authors.forename: Z H
authors.initials: ZH
authors.lastname: Li
authors.forename: J H
authors.initials: JH
authors.lastname: Zhang
authors.forename: X R
authors.initials: XR
authors.lastname: Wang
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis* / Q000276:immunology; D000918:Antibody Specificity; D002364:Caseins / Q000032:analysis*; D002417:Cattle; D007118:Immunoassay / Q000295:instrumentation / Q000379:methods*; D008892:Milk / Q000737:chemistry*
chemical_list: D000911:Antibodies, Monoclonal; D002364:Caseins