title: Comparative phenotypic characterization of human cord blood monocytes and placental macrophages at term.
abstract: The expression of surface molecules in cord blood monocytes and placental macrophages was studied using flow cytometry. When compared with monocytes, macrophages presented a decrease in HLA-DR and LAP/TGF-β1 levels and increased expression of alternative activation markers, especially CD206. No difference in the production of the apoptotic factors TRAIL and TWEAK was observed, whereas the levels of cytokine receptors in monocytes were significantly higher than in macrophages. Most remarkable was the difference in the expression of IL-17 and TNFα receptors. A strong correlation between VEGF and TNFα receptors was revealed in both cell populations. The results obtained in this study provide antigenic phenotypes for two related cell populations and outline the feasible functional alterations during tissue macrophage differentiation.
authors.affiliation: D.O. Ott Research Institute for Obstetrics and Gynecology, St. Petersburg, Russia.
authors.forename: S A
authors.initials: SA
authors.lastname: Selkov
authors.forename: A V
authors.initials: AV
authors.lastname: Selutin
authors.forename: O M
authors.initials: OM
authors.lastname: Pavlova
authors.forename: N N
authors.initials: NN
authors.lastname: Khromov-Borisov
authors.forename: O V
authors.initials: OV
authors.lastname: Pavlov
mesh_terms: D000943:Antigens, Differentiation / Q000378:metabolism*; D002454:Cell Differentiation; D002478:Cells, Cultured; D002585:Cesarean Section; D016207:Cytokines / Q000378:metabolism; D005260:Female; D005312:Fetal Blood / Q000166:cytology; D005326:Fetal Proteins / Q000378:metabolism*; D005434:Flow Cytometry; D018507:Gene Expression Regulation, Developmental; D006684:HLA-DR Antigens / Q000378:metabolism; D006801:Humans; D037181:Lectins, C-Type / Q000378:metabolism; D008264:Macrophages / Q000166:cytology / Q000276:immunology / Q000378:metabolism*; D000090323:Mannose Receptor; D037241:Mannose-Binding Lectins / Q000378:metabolism; D009000:Monocytes / Q000166:cytology / Q000276:immunology / Q000378:metabolism*; D010920:Placenta / Q000166:cytology* / Q000276:immunology; D011247:Pregnancy; D011263:Pregnancy Trimester, Third; D011956:Receptors, Cell Surface / Q000378:metabolism; D018121:Receptors, Cytokine / Q000378:metabolism; D013499:Surface Properties
chemical_list: D000943:Antigens, Differentiation; D016207:Cytokines; D005326:Fetal Proteins; D006684:HLA-DR Antigens; D037181:Lectins, C-Type; D000090323:Mannose Receptor; D037241:Mannose-Binding Lectins; D011956:Receptors, Cell Surface; D018121:Receptors, Cytokine
keywords: Apoptotic factors; Cord blood monocytes; Cytokine receptors; Differentiation markers; Placental macrophages