title: Talbot-defocus multiscan tomography using the synchrotron X-ray microscope to study the lacuno-canalicular network in mouse bone.
abstract: The three-dimensional network of lacunae and canaliculi that regulates metabolism in bone contains osteocytes and their dendritic processes. We constructed a synchrotron radiation X-ray microscope for sequential tomography of mouse tibia first by using a Talbot interferometer to detect the degree of bone mineralization and then by using absorption contrast under a slightly defocused setting to enhance outline contrast thereby visualizing structures of the osteocyte lacuno-canalicular network. The resultant pair of tomograms was precisely aligned with each other, allowing evaluation of mineral density in the vicinity of each osteocyte lacuna and canaliculus over the entire thickness of the cortical bone. Thus, multiscan microscopic X-ray tomography is a powerful tool for analyzing bone mineralization in relation to the lacuno-canalicular network at the submicron resolution level.
authors.affiliation: Ratoc System Engineering Co., Ltd, 1-24-8 Sekiguchi, Bunkyo-ku, Tokyo 112-0014, Japan.
authors.forename: Nobuhito
authors.initials: N
authors.lastname: Nango
authors.forename: Shogo
authors.initials: S
authors.lastname: Kubota
authors.forename: Akihisa
authors.initials: A
authors.lastname: Takeuchi
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Suzuki
authors.forename: Wataru
authors.initials: W
authors.lastname: Yashiro
authors.forename: Atsushi
authors.initials: A
authors.lastname: Momose
authors.forename: Koichi
authors.initials: K
authors.lastname: Matsuo
keywords: (070.6760) Talbot and self-imaging effects; (110.6960) Tomography; (180.7460) X-ray microscopy; (340.6720) Synchrotron radiation