title: Fibroblast activation protein-α promotes ovarian cancer cell proliferation and invasion via extracellular and intracellular signaling mechanisms.
abstract: Fibroblast activation protein-α (FAPα) is secreted by activated stromal fibroblasts and can promote ovarian cancer cell proliferation, migration and invasion. However, the molecular mechanism by which FAPα promotes tumor cell proliferation and invasion is unknown. The role of the non-enzymatic activities of FAPα in tumor migration and invasion and the intracellular and extracellular signaling mechanisms of FAPα were investigated. In this study, we confirm that FAPα promote ovarian cancer cell proliferation, migration and invasion by extracellular and intracellular signaling mechanisms. These results provide evidence that FAPα, together with integrin α3β1 and the uPAR signaling complex, mediate cancer cell migration in the HO-8910PM cell line via the small GTPase Rac1. FAPα-mediated upregulation of p-ERK occurred in a time-dependent manner.
authors.affiliation: Department of Pathology, Harbin Medical University, Harbin 150081, China.
authors.forename: WeiWei
authors.initials: W
authors.lastname: Yang
authors.forename: Wei
authors.initials: W
authors.lastname: Han
authors.forename: ShengQian
authors.initials: S
authors.lastname: Ye
authors.forename: DuanYang
authors.initials: D
authors.lastname: Liu
authors.forename: Jun
authors.initials: J
authors.lastname: Wu
authors.forename: HongYu
authors.initials: H
authors.lastname: Liu
authors.forename: ChunHong
authors.initials: C
authors.lastname: Li
authors.forename: He
authors.initials: H
authors.lastname: Chen
mesh_terms: D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D010450:Endopeptidases; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D005260:Female; D018093:Gelatinases / Q000378:metabolism*; D006801:Humans; D039201:Integrin alpha3beta1 / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism*; D010051:Ovarian Neoplasms / Q000378:metabolism* / Q000473:pathology*; D010766:Phosphorylation; D055503:Protein Multimerization; D055293:Receptors, Urokinase Plasminogen Activator / Q000378:metabolism; D012697:Serine Endopeptidases / Q000378:metabolism*; D015398:Signal Transduction; D020830:rac1 GTP-Binding Protein / Q000378:metabolism; D054460:rho-Associated Kinases / Q000378:metabolism; D000099167:Fibroblast Activation Protein Alpha
chemical_list: D010450:Endopeptidases; D048049:Extracellular Signal-Regulated MAP Kinases; D018093:Gelatinases; D039201:Integrin alpha3beta1; D008565:Membrane Proteins; D055293:Receptors, Urokinase Plasminogen Activator; D012697:Serine Endopeptidases; D020830:rac1 GTP-Binding Protein; D054460:rho-Associated Kinases; D000099167:Fibroblast Activation Protein Alpha; C107953:RAC1 protein, human
keywords: Fibroblast activation protein-α (FAPα); Integrin α3β1; Ovarian cancer; Urokinase-type plasminogen activator receptor (uPAR)