title: In vitro cytotoxicity of white MTA, MTA Fillapex® and Portland cement on human periodontal ligament fibroblasts.
abstract: The aim of this study was to compare the in vitro cytotoxicity of white mineral trioxide aggregate (MTA), MTA Fillapex® and Portland cement (PC) on human cultured periodontal ligament fibroblasts. Periodontal ligament fibroblast culture was established and the cells were used for cytotoxic tests after the fourth passage. Cell density was set at 1.25 X10 4 cells/well in 96-well plates. Endodontic material extracts were prepared by placing sealer/cement specimens (5x3mm) in 1mL of culture medium for 72 h. The extracts were then serially two-fold diluted and inserted into the cell-seeded wells for 24, 48 and 72 h. MTT assay was employed for analysis of cell viability. Cell supernatants were tested for nitric oxide using the Griess reagent system. MTA presented cytotoxic effect in undiluted extracts at 24 and 72 h. MTA Fillapex® presented the highest cytotoxic levels with important cell viability reduction for pure extracts and at ½ and ¼ dilutions. In this study, PC did not induce alterations in fibroblast viability. Nitric oxide was detected in extract-treated cell supernatants and also in the extracts only, suggesting presence of nitrite in the soluble content of the tested materials. In the present study, MTA Fillapex displayed the highest cytotoxic effect on periodontal ligament fibroblasts followed by white MTA and PC.
authors.affiliation: Endodontics Division, Hospital for Rehabilitation of Craniofacial Anomalies, University of São Paulo (HRAC-USP), Bauru, SP, Brazil.
authors.forename: Patrícia
authors.initials: P
authors.lastname: Yoshino
authors.forename: Celso Kenji
authors.initials: CK
authors.lastname: Nishiyama
authors.forename: Karin Cristina da Silva
authors.initials: KC
authors.lastname: Modena
authors.forename: Carlos Ferreira
authors.initials: CF
authors.lastname: Santos
authors.forename: Carla Renata
authors.initials: CR
authors.lastname: Sipert
mesh_terms: D017607:Aluminum Compounds / Q000633:toxicity*; D017610:Calcium Compounds / Q000633:toxicity*; D002452:Cell Count; D018929:Cell Culture Techniques; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004396:Coloring Agents; D003738:Dental Cements / Q000633:toxicity*; D004338:Drug Combinations; D005290:Ferric Compounds / Q000633:toxicity; D005347:Fibroblasts / Q000187:drug effects; D006801:Humans; D008422:Materials Testing; D009569:Nitric Oxide / Q000032:analysis; D009573:Nitrites / Q000633:toxicity; D010087:Oxides / Q000633:toxicity*; D010513:Periodontal Ligament / Q000166:cytology / Q000187:drug effects*; D012387:Root Canal Filling Materials / Q000633:toxicity*; D017640:Silicates / Q000633:toxicity*; D013778:Tetrazolium Salts; D013844:Thiazoles; D013997:Time Factors
chemical_list: D017607:Aluminum Compounds; D017610:Calcium Compounds; D004396:Coloring Agents; D003738:Dental Cements; D004338:Drug Combinations; D005290:Ferric Compounds; D009573:Nitrites; D010087:Oxides; D012387:Root Canal Filling Materials; D017640:Silicates; D013778:Tetrazolium Salts; D013844:Thiazoles; C086631:mineral trioxide aggregate; C013481:dicalcium silicate; C035219:calcium aluminate; C035218:calcium aluminate ferrite; D009569:Nitric Oxide; C506393:tricalcium silicate; C022616:thiazolyl blue