title: Purification and characterization of a novel anti-HSV-2 protein with antiproliferative and peroxidase activities from Stellaria media.
abstract: A novel antiviral protein, designated as Stellarmedin A, was purified from Stellaria media (L.) Vill. (Caryophyllaceae) by using ammonium sulfate precipitation, cation-exchange chromatography system. Gel electrophoresis analysis showed that Stellarmedin A is a highly basic glycoprotein with a molecular weight of 35.1 kDa and an isoelectric point of ∼8.7. The N-terminal 14-amino acid sequence, MGNTGVLTGERNDR, is similar to those of other plant peroxidases. This protein inhibited herpes simplex virus type 2 (HSV-2) replication in vitro with an IC50 of 13.18 µg/ml and a therapeutic index exceeding 75.9. It was demonstrated that Stellarmedin A affects the initial stage of HSV-2 infection and is able to inhibit the proliferation of promyelocytic leukemia HL-60 and colon carcinoma LoVo cells with an IC50 of 9.09 and 12.32 µM, respectively. Moreover, Stellarmedin A has a peroxidase activity of 36.6 µmol/min/mg protein, when guaiacol was used as substrate. To our knowledge, this is the first report about an anti-HSV-2 protein with antiproliferative and peroxidase activities from S. media.
authors.affiliation: Jiangsu Center for Research & Development of Medicinal Plants, Institute of Botany, Jiangsu Province and Chinese Academy of Sciences (Nanjing Botanical Garden, Mem. Sun Yat-sen), Nanjing 210014, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Shan
authors.forename: Yuhong
authors.initials: Y
authors.lastname: Zheng
authors.forename: Fuqin
authors.initials: F
authors.lastname: Guan
authors.forename: Jianjian
authors.initials: J
authors.lastname: Zhou
authors.forename: Haiguang
authors.initials: H
authors.lastname: Zhao
authors.forename: Bing
authors.initials: B
authors.lastname: Xia
authors.forename: Xu
authors.initials: X
authors.lastname: Feng
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000998:Antiviral Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D049109:Cell Proliferation / Q000187:drug effects*; D002522:Chlorocebus aethiops; D018258:Herpesvirus 2, Human / Q000187:drug effects*; D008969:Molecular Sequence Data; D010544:Peroxidases / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism* / Q000494:pharmacology; D010940:Plant Proteins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D029752:Stellaria / Q000378:metabolism*; D014709:Vero Cells
chemical_list: D000998:Antiviral Agents; D010940:Plant Proteins; D010544:Peroxidases; C586715:stellarmedin A protein, Stellaria media
keywords: Stellaria media; Stellarmedin A; anti-HSV-2; antiproliferative; peroxidase