title: Sodium houttuyfonate, a potential phytoanticipin derivative of antibacterial agent, inhibits bacterial attachment and pyocyanine secretion of Pseudomonas aeruginosa by attenuating flagella-mediated swimming motility.
abstract: Pseudomonas aeruginosa is a well-known clinical pathogen for its recalcitrant infection caused by biofilm formation which are initiated by flagella-mediated attachment. Sodium houttuyfonate (SH) is a natural phytoanticipin derivative of houttuynin and has anti-pathogenic effect on P. aeruginosa biofilm formation. In this paper, when using 1/2 × MIC SH, the diameter of P. aeruginosa swimming motility was sharply shortened to 36 % in 24 h incubation, and the fold changes of fliC required for swimming motility was 0.36 in 24 h cultivation, the adherence inhibition accounted for about 46 %, and the pyocyanin production decreased to 47 % after 1-day treatment and 56 % after 3-day treatment with obvious visual changes from dark green to light green, compared with the negative control. With the help of mass spectra and scanning electronic microscope, 1/2 × MIC SH was further testified to be enough to eradicate flagella and inhibit pyocyanin secretion of P. aeruginosa. The results do not only re-affirm the close interplay of attachment and virulence (i.e. swimming motility and pyocyanin), but also unravel the potential mechanism of SH on anti-biofilm of P. aeruginosa.
authors.affiliation: Laboratory of Microbiology and Immunology, School of Chinese and Western Integrative Medicine, Anhui University of Chinese Medicine, Hefei, 230038, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Shao
authors.forename: Huijuan
authors.initials: H
authors.lastname: Cheng
authors.forename: Changzhong
authors.initials: C
authors.lastname: Wang
authors.forename: Daqiang
authors.initials: D
authors.lastname: Wu
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Zhu
authors.forename: Lingling
authors.initials: L
authors.lastname: Zhu
authors.forename: Zhenxin
authors.initials: Z
authors.lastname: Sun
mesh_terms: D000473:Alkanes / Q000494:pharmacology*; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D018441:Biofilms / Q000187:drug effects / Q000254:growth & development; D005407:Flagella / Q000187:drug effects* / Q000502:physiology; D008826:Microbial Sensitivity Tests; D008855:Microscopy, Electron, Scanning; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000235:genetics / Q000472:pathogenicity / Q000502:physiology; D011710:Pyocyanine / Q000378:metabolism*; D013447:Sulfites / Q000494:pharmacology*; D014774:Virulence
chemical_list: D000473:Alkanes; D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D013447:Sulfites; C473296:sodium houttuyfonate; D011710:Pyocyanine