title: Involvement of poly(3-hydroxybutyrate) synthesis in catabolite repression of tetralin biodegradation genes in Sphingomonas macrogolitabida strain TFA.
abstract: The tetralin biodegradation genes of Sphingomonas macrogolitabida strain TFA are repressed by catabolite. Insertion mutants in which thn genes are transcribed in the presence of a preferential carbon source and tetralin, bear the insertion in phaC, encoding a poly(3-hydroxybutyrate) (PHB) synthase, a key enzyme in PHB synthesis. Mutant complementation with phaC genes from either Ralstonia euthropha or TFA restored PHB accumulation and the wild-type regulatory pattern of thn genes, thus indicating that this accumulation is a signal for carbon sufficient conditions that prevents expression of thn catabolic genes in this α-proteobacteria.
authors.affiliation: Centro Andaluz de Biología del Desarrollo, CSIC-Universidad Pablo de Olavide, Seville, Spain Centro de Investigaciones Biológicas, CSIC, 28040 Madrid, Spain.
authors.forename: Guadalupe
authors.initials: G
authors.lastname: Martín-Cabello
authors.forename: Emilia
authors.initials: E
authors.lastname: Moreno-Ruiz
authors.forename: Valle
authors.initials: V
authors.lastname: Morales
authors.forename: Belén
authors.initials: B
authors.lastname: Floriano
authors.forename: Eduardo
authors.initials: E
authors.lastname: Santero