title: Guided assembly of tetramolecular G-quadruplexes.
abstract: Nucleic acids are finding applications in nanotechnology as nanomaterials, mechanical devices, templates, and biosensors. G-quadruplex DNA, formed by π-π stacking of guanine (G) quartets, is an attractive alternative to regular B-DNA because of the kinetic and thermodynamic stability of quadruplexes. However, they suffer from a fatal flaw: the rules of recognition, i.e., the formation of a G-quartet in which four identical bases are paired, prevent the controlled assembly between different strands, leading to complex mixtures. In this report, we present the solution to this recognition problem. The proposed design combines two DNA elements: parallel-stranded duplexes and a quadruplex core. Parallel-stranded duplexes direct controlled assembly of the quadruplex core, and their strands present convenient points of attachments for potential modifiers. The exceptional stability of the quadruplex core provides integrity to the entire structure, which could be used as a building block for nucleic acid-based nanomaterials. As a proof of principle for the design's versatility, we assembled quadruplex-based 1D structures and visualized them using atomic force and transmission electron microscopy. Our findings pave the way to broader utilization of G-quadruplex DNA in structural DNA nanomaterials.
authors.affiliation: ARNA Laboratory, University of Bordeaux, F-33000 Bordeaux, France. lyatsun1@swarthmore.edu
authors.forename: Liliya A
authors.initials: LA
authors.lastname: Yatsunyk
authors.forename: Olivier
authors.initials: O
authors.lastname: Piétrement
authors.forename: Delphine
authors.initials: D
authors.lastname: Albrecht
authors.forename: Phong Lan Thao
authors.initials: PL
authors.lastname: Tran
authors.forename: Daniel
authors.initials: D
authors.lastname: Renčiuk
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Sugiyama
authors.forename: Jean-Michel
authors.initials: JM
authors.lastname: Arbona
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Aimé
authors.forename: Jean-Louis
authors.initials: JL
authors.lastname: Mergny
mesh_terms: D003460:Crystallization / Q000379:methods*; D004247:DNA / Q000737:chemistry* / Q000648:ultrastructure*; D054856:G-Quadruplexes; D046911:Macromolecular Substances / Q000737:chemistry; D008422:Materials Testing; D008968:Molecular Conformation; D013499:Surface Properties
chemical_list: D046911:Macromolecular Substances; D004247:DNA