title: ADP is a vasodilator component from Lasiodora sp. mygalomorph spider venom.
abstract: Members of the spider genus Lasiodora are widely distributed in Brazil, where they are commonly known as caranguejeiras. Lasiodora spider venom is slightly harmful to humans. The bite of this spider causes local pain, edema and erythema. However, Lasiodora sp. spider venom may be a source of important pharmacological tools. Our research group has described previously that Lasiodora sp. venom produces bradycardia in the isolated rat heart. In the present work, we sought to evaluate the vascular effect of Lasiodora sp. venom and to isolate the vasoactive compounds from the venom. The results showed that Lasiodora spider venom induced a concentration-dependent vasodilation in rat aortic rings, which was dependent on the presence of a functional endothelium and abolished by the nitric oxide synthase (NOS) inhibitor L-NAME. Western blot experiments revealed that the venom also increased endothelial NOS function by increasing phosphorylation of the Ser¹¹⁷⁷ residue. Assay-directed fractionation isolated a vasoactive fraction from Lasiodora sp. venom. Mass spectrometry (MS) and nuclear magnetic resonance (NMR) assays identified a mixture of two compounds: adenosine diphosphate (ADP, approximately 90%) and adenosine monophosphate (AMP, approximately 10%). The vasodilator effects of Lasiodora sp. whole venom, as well as ADP, were significantly inhibited by suramin, which is a purinergic P2-receptor antagonist. Therefore, the results of the present work indicate that ADP is a main vasodilator component of Lasiodora sp. spider venom.
authors.affiliation: Departamento de Biologia Geral, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Av. Antônio Carlos 6627, Pampulha, Belo Horizonte 31270-901, Minas Gerais, Brazil.
authors.forename: C C
authors.initials: CC
authors.lastname: Horta
authors.forename: B A
authors.initials: BA
authors.lastname: Rezende
authors.forename: B B R
authors.initials: BB
authors.lastname: Oliveira-Mendes
authors.forename: A O
authors.initials: AO
authors.lastname: Carmo
authors.forename: L S A
authors.initials: LS
authors.lastname: Capettini
authors.forename: J F
authors.initials: JF
authors.lastname: Silva
authors.forename: M T
authors.initials: MT
authors.lastname: Gomes
authors.forename: C
authors.initials: C
authors.lastname: Chávez-Olórtegui
authors.forename: C E S
authors.initials: CE
authors.lastname: Bravo
authors.forename: V S
authors.initials: VS
authors.lastname: Lemos
authors.forename: E
authors.initials: E
authors.lastname: Kalapothakis
mesh_terms: D000244:Adenosine Diphosphate / Q000737:chemistry / Q000494:pharmacology*; D000249:Adenosine Monophosphate / Q000737:chemistry; D000818:Animals; D015153:Blotting, Western; D005591:Chemical Fractionation; D004727:Endothelium / Q000187:drug effects; D066298:In Vitro Techniques; D013058:Mass Spectrometry; D019331:NG-Nitroarginine Methyl Ester / Q000378:metabolism; D019001:Nitric Oxide Synthase / Q000378:metabolism; D019906:Nuclear Magnetic Resonance, Biomolecular; D010766:Phosphorylation / Q000187:drug effects; D051381:Rats; D013111:Spider Venoms / Q000737:chemistry*; D013112:Spiders / Q000737:chemistry*; D013498:Suramin / Q000737:chemistry; D014664:Vasodilation / Q000187:drug effects; D014665:Vasodilator Agents / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D013111:Spider Venoms; D014665:Vasodilator Agents; D000249:Adenosine Monophosphate; D013498:Suramin; D000244:Adenosine Diphosphate; D019001:Nitric Oxide Synthase; D019331:NG-Nitroarginine Methyl Ester
keywords: ADP; Lasiodora; Nitric oxide; Spider; Vasodilation; Venom