title: Modeling and optimization of glutamic acid production using mixed culture of Corynebacterium glutamicum NCIM2168 and Pseudomonas reptilivora NCIM2598.
abstract: In this study, a hybrid system of response surface methodology followed by genetic algorithm has been adopted to optimize the production medium for L-glutamic acid fermentation with mixed cultures of Corynebacterium glutamicum and Pseudomonas reptilovora. The optimal combination of media components for maximal production of L-glutamic acid was found to be 49.99 g L(-1) of glucose, 10 g L(-1) of urea, 18.06% (v/v) of salt solution, and 4.99% (v/v) of inoculum size. The experimental glutamic acid yield at optimum condition was 19.69 g L(-1), which coincided well to the value predicted by the model (19.61 g L(-1)). Using this methodology, a nonlinear regression model was developed for the glutamic acid production. The model was validated statistically and the determination coefficient (R (2)) was found to be 0.99.
authors.affiliation: Department of Biotechnology, Centre for Research, Bioprocess and Downstream Processing Laboratory, Kamaraj College of Engineering and Technology, Virudhunagar, Tamilnadu, India. kingshyam2003@yahoo
authors.forename: Rajaram Shyam
authors.initials: RS
authors.lastname: Kumar
authors.forename: Innasi Muthu Ganesh
authors.initials: IM
authors.lastname: Moorthy
authors.forename: Rajoo
authors.initials: R
authors.lastname: Baskar
mesh_terms: D001709:Biotechnology / Q000379:methods*; D048230:Corynebacterium glutamicum / Q000254:growth & development / Q000378:metabolism*; D003470:Culture Media; D005285:Fermentation; D005947:Glucose / Q000378:metabolism; D018698:Glutamic Acid / Q000096:biosynthesis* / Q000737:chemistry; D011549:Pseudomonas / Q000254:growth & development / Q000378:metabolism*
chemical_list: D003470:Culture Media; D018698:Glutamic Acid; D005947:Glucose