title: Isolation of high-quality RNA from stented blood vessels.
abstract: Stents have become a standard of care for the treatment of coronary artery disease. A series of cellular and molecular processes contribute to the vascular response following stent placement. For the purpose of local gene expression studies, metallic stent struts are usually removed from the vessel wall with forceps under a dissection microscope prior to RNA extraction. Main drawbacks of the manual dissection are that it may cause additional tissue damage and compromise the quality of RNA through prolonged tissue handling. In this technical note, we report the recovery of high-quality RNA from atherosclerotic vessels with stent struts left in situ.
authors.affiliation: Laboratory for Cellular and Molecular Cardiology, Department of Cardiology, Antwerp University Hospital, Antwerp, Belgium.
authors.forename: Christophe J
authors.initials: CJ
authors.lastname: Van Dyck
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Timmermans
authors.forename: Erik
authors.initials: E
authors.lastname: Fransen
authors.forename: Christiaan J
authors.initials: CJ
authors.lastname: Vrints
authors.forename: Vicky Y
authors.initials: VY
authors.lastname: Hoymans
mesh_terms: D000818:Animals; D050197:Atherosclerosis / Q000378:metabolism* / Q000628:therapy; D003331:Coronary Vessels / Q000502:physiology*; D054855:Drug-Eluting Stents; D020869:Gene Expression Profiling / Q000379:methods; D008297:Male; D008670:Metals; D012313:RNA / Q000032:analysis / Q000302:isolation & purification*; D012337:RNA, Ribosomal, 18S / Q000032:analysis / Q000302:isolation & purification; D012339:RNA, Ribosomal, 28S / Q000032:analysis / Q000302:isolation & purification; D011817:Rabbits; D015607:Stents
chemical_list: D008670:Metals; D012337:RNA, Ribosomal, 18S; D012339:RNA, Ribosomal, 28S; D012313:RNA