title: Unveiling the rat urinary proteome with three complementary proteomics approaches.
abstract: Urine is a suitable biological fluid to look for markers of physiological and pathological processes, including renal and nonrenal diseases. In addition, it is an optimal body sample for diagnosis, because it is easily obtained without invasive procedures and can be sampled in large quantities at almost any time. Rats are frequently used as a model to study human diseases, and rat urine has been analyzed to search for disease biomarkers. The normal human urinary proteome has been studied extensively, but the normal rat urinary proteome has not been studied in such depth. In light of this, we were prompted to analyze the normal rat urinary proteome using three complementary proteomics platforms: SDS-PAGE separation, followed by LC-ESI-MS/MS; 2DE, followed by MALDI-TOF-TOF and 2D-liquid chromatography-chromatofocusing, followed by LC-ESI-Q-TOF. A total of 366 unique proteins were identified, of which only 5.2% of unique proteins were identified jointly by the three proteomics platforms used. This suggests that simultaneous proteomics techniques provide complementary and nonredundant information. Our analysis affords the most extensive rat urinary protein database currently available and this may be useful in the study of renal physiology and in the search for biomarkers related to renal and nonrenal diseases.
authors.affiliation: Unidad de Investigación, Hospital Universitario de Salamanca, Salamanca, Spain.
authors.forename: Fernando
authors.initials: F
authors.lastname: Sánchez-Juanes
authors.forename: María Carmen
authors.initials: MC
authors.lastname: Muñiz
authors.forename: César
authors.initials: C
authors.lastname: Raposo
authors.forename: Silvia
authors.initials: S
authors.lastname: Rodríguez-Prieto
authors.forename: Alberto
authors.initials: A
authors.lastname: Paradela
authors.forename: Yaremi
authors.initials: Y
authors.lastname: Quiros
authors.forename: Francisco
authors.initials: F
authors.lastname: López-Hernández
authors.forename: José Manuel
authors.initials: JM
authors.lastname: González-Buitrago
authors.forename: Laura
authors.initials: L
authors.lastname: Ferreira
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000737:chemistry / Q000652:urine*; D002853:Chromatography, Liquid / Q000379:methods; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D005260:Female; D006863:Hydrogen-Ion Concentration; D013058:Mass Spectrometry / Q000379:methods; D011506:Proteins / Q000737:chemistry / Q000145:classification; D011507:Proteinuria / Q000652:urine*; D020543:Proteome / Q000032:analysis* / Q000737:chemistry; D040901:Proteomics / Q000379:methods*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D015415:Biomarkers; D011506:Proteins; D020543:Proteome
keywords: MALDI-TOF; PF2D; Rat urine; Two-dimensional electrophoresis; Urinary proteome