title: [Simultaneous determination of four trace aconitum alkaloids in urine using ultra performance liquid chromatography-mass spectrometry].
abstract: A rapid, specific and sensitive ultra performance liquid chromatography-mass spectrometry (UPLC-MS/MS) method was developed for the simultaneous determination of four trace alkaloids, including aconitine (AC), hypaconitine (HA), mesaconitine (MA) and yunaconitine (YA) in human urine samples. UPLC-MS-MS system coupled with an electrospray ionization (ESI) source was performed in multiple reaction monitoring (MRM) mode. The sample preparation was performed with hollow fiber microextraction (HF-LPME) prior to the analysis. The enrichment factors of the four alkaloids were 102 - 301. The separation was applied on a Waters ACQUITY UPLC BEH C18 column (50 mm x 2.1 mm, 1.7 micro m) with a gradient elution of acetonitrile and 10 mmol/L NH4HCO3, as mobile phase. The retention times , were less than 3 min. This method significantly improved the detection sensitivity, and the limits of quantitation were from 0. 01 to 0.1 ng/L. The calibration curves were linear over the ranges of 0.01 - 10 ng/L for AC, MA and YA, 0.1 - 100 ng/L for HA in human urine samples, and the correlation coefficients were 0. 998 1, 0.998 4, 0.999 5 and 0.998 6, respectively. The method was proved to be rapid and sensitive for aconitum alkaloid analysis in urine samples.
authors.affiliation: School of Pharmacology, Hebei Medical University, Shijiazhuang 050017, China.
authors.forename: Panpan
authors.initials: P
authors.lastname: Zhang
authors.forename: Fucheng
authors.initials: F
authors.lastname: Zhang
authors.forename: Zhaohong
authors.initials: Z
authors.lastname: Wang
authors.forename: Ye
authors.initials: Y
authors.lastname: Jiang
authors.forename: Yongjiang
authors.initials: Y
authors.lastname: Lu
mesh_terms: D000157:Aconitine / Q000031:analogs & derivatives* / Q000652:urine*; D031841:Aconitum / Q000737:chemistry*; D000470:Alkaloids / Q000652:urine; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D018517:Plant Roots / Q000737:chemistry
chemical_list: D000470:Alkaloids; D004365:Drugs, Chinese Herbal; D000157:Aconitine