title: New fluphenazine analogues as inhibitors of P-glycoprotein in human lymphocyte cultures.
abstract: OBJECTIVE
  To evaluate the inhibitory effect of 17 new analogues of FPh on the Pgp transport function, by estimation of the rhodamine 123 (Rod-123) accumulation inside cultured lymphocytes.
  
  
  METHODS
  Lymphocyte were cultured in the presence of a lectin (PHA; 2%, v/v), incubated with benzo[α]pyrene (B[α]P; 7.5 µM, 48 h) to induce genotoxic damage and to increase Pgp expression in the cells. Lymphocytes cultured without the tested compounds were considered as controls.
  
  
  RESULTS
  It was established that 10 analogues of FPh, among 17 tested, significantly increased Rod-123 accumulation in lymphocytes at the concentration of 10 µM. As compared to the control cultures the Pgp transport function was the most strongly inhibited by 1a, 1b, 1d, 3f, 3h and 3i analogues (approximately by 25%).
  
  
  CONCLUSIONS
  FPh analogues 1a, 1b, 1d, 3f, 3h and 3i should be further studied as promising candidates for adjuvant cancer chemotherapeutics.
authors.affiliation: Department of Basic Medical Sciences, Wrocław Medical University, Wrocław, Poland.
authors.forename: Agata
authors.initials: A
authors.lastname: Jaszczyszyn
authors.forename: Kazimierz
authors.initials: K
authors.lastname: Gąsiorowski
authors.forename: Piotr
authors.initials: P
authors.lastname: Swiątek
authors.forename: Wiesław
authors.initials: W
authors.lastname: Malinka
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Cieślik-Boczula
authors.forename: Joanna
authors.initials: J
authors.lastname: Petrus
authors.forename: Bogusława
authors.initials: B
authors.lastname: Czarnik-Matusewicz
keywords: MDR; MDR modulators; P-glycoprotein; fluphenazine analogues