title: Variable spatial magnetic field influences peripheral nerves regeneration in rats.
abstract: Generator of spatial magnetic field is one of most recent achievements among the magnetostimulators. This apparatus allows to obtain the rotating magnetic field. This new method may be more effective than other widely used techniques of magnetostimulation and magnetotherapy. We investigated the influence of alternating, spatial magnetic field on the regeneration of the crushed rat sciatic nerves. Functional and morphological evaluations were used. After crush injury of the right sciatic nerve, Wistar C rats (n = 80) were randomly divided into four groups (control and three experimental). The experimental groups (A, B, C) were exposed (20 min/day, 5 d/week, 4 weeks) to alternating spatial magnetic field of three different intensities. Sciatic Functional Index (SFI) and tensometric assessments were performed every week after nerve crush. Forty-eight hours before the sacrificing of animals, DiI (1,1'-di-octadecyl-3,3,3',3'-tetramethyloindocarbocyanine perchlorate) was applied 5 mm distally to the crush site. Collected nerves and dorsal root ganglia (DRG) were subjected to histological and immunohistochemical staining. The survival rate of DRG neurons was estimated. Regrowth and myelination of the nerves was examined. The results of SFI and tensometric assessment showed improvement in all experimental groups as compared to control, with best outcome observed in group C, exposed to the strongest magnetic field. In addition, DRG survival rate and nerve regeneration intensity were significantly higher in the C group. Above results indicate that strong spatial alternating magnetic field exerts positive effect on peripheral nerve regeneration and its application could be taken under consideration in the therapy of injured peripheral nerves.
authors.affiliation: Department of Physiology, Medical University of Silesia , Katowice , Poland and.
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Suszyński
authors.forename: Wiesław
authors.initials: W
authors.lastname: Marcol
authors.forename: Sebastian
authors.initials: S
authors.lastname: Szajkowski
authors.forename: Marita
authors.initials: M
authors.lastname: Pietrucha-Dutczak
authors.forename: Grzegorz
authors.initials: G
authors.lastname: Cieślar
authors.forename: Aleksander
authors.initials: A
authors.lastname: Sieroń
authors.forename: Joanna
authors.initials: J
authors.lastname: Lewin-Kowalik
mesh_terms: D000818:Animals; D001835:Body Weight; D005121:Extremities / Q000294:innervation / Q000601:surgery; D005727:Ganglia, Spinal / Q000166:cytology / Q000502:physiology*; D060526:Magnetic Fields; D008297:Male; D009416:Nerve Regeneration; D051381:Rats; D017208:Rats, Wistar; D012584:Sciatic Nerve / Q000166:cytology / Q000502:physiology*
keywords: Electromagnetic fields; magnetic field therapy; nerve regeneration; rat; sciatic nerve