title: Evaluating the performance of different multicolumn setups for chromatographic separation of proteins on hydrophobic interaction chromatography media by a numerical study.
abstract: A theoretical study has been performed on the effectiveness of isolating a target component out of a multi-component protein mixture using different arrangements of chromatographic columns. Three continuous systems have been considered which were able to perform solvent gradient separations, such as: open loop simulated moving bed, countercurrent solvent gradient purification and carousel multicolumn setup. The performance of the continuous processes was examined with respect to productivity, yield and eluent consumption and compared to a single-column batch system. As a case study separation of a ternary mixture of proteins on HIC media has been selected. Two separation problems have been analyzed referring to the situation when the target component was the most strongly adsorbed as well as when it exhibited intermediate adsorption strength. A mathematical model has been used to simulate the process dynamics and to optimize operating conditions for the separation. The numerical study indicated that batch column arrangements can outperform SMB-based configurations regarding all performance indicators considered, which has been attributed to solvent mixing in the recycled streams and distortion of the gradient shape in SMB units. It has been concluded that the performance of complex multicolumn systems should be verified vs. batch column operations prior to the realization of the separation process.
authors.affiliation: Department of Chemical and Process Engineering, Rzeszow University of Technology, Powstancow Warszawy Avenue 6, 35-959 Rzeszow, Poland.
authors.forename: Roman
authors.initials: R
authors.lastname: Bochenek
authors.forename: Wojciech
authors.initials: W
authors.lastname: Marek
authors.forename: Wojciech
authors.initials: W
authors.lastname: Piątkowski
authors.forename: Dorota
authors.initials: D
authors.lastname: Antos
mesh_terms: D002853:Chromatography, Liquid / Q000295:instrumentation* / Q000379:methods*; D003198:Computer Simulation; D057927:Hydrophobic and Hydrophilic Interactions; D008956:Models, Chemical; D011506:Proteins / Q000737:chemistry / Q000302:isolation & purification*; D012492:Salts / Q000737:chemistry; D012997:Solvents / Q000737:chemistry
chemical_list: D011506:Proteins; D012492:Salts; D012997:Solvents
keywords: Carousel multicolumn setup; Countercurrent solvent gradient purification process; HIC; Optimization; Protein separations; SMB