title: Molecular identification and analysis of human enteroviruses isolated from healthy children in Shenzhen, China from 2010 to 2011.
abstract: OBJECTIVE
  To determine the prevalence and distribution of human enteroviruses (HEVs) among healthy children in Shenzhen, China.
  
  
  METHODS
  Clinical specimens were obtained from 320 healthy children under 5 years old in Shenzhen, China from 2010 to 2011. The specimens were evaluated using real-time PCR and cell cultures. The positive specimens were further tested using reverse transcription-seminested PCR (RT-snPCR). Molecular typing and phylogenetic analysis were based on the sequence determined.
  
  
  RESULTS
  Among the 320 samples, 34 were tested positive for HEVs (10.6%) and 22 different serotypes were identified using RT-snPCR. PV1 and PV2 were also detected. The predominant serotype observed was EV71 (17.6%), followed by CV-B4 (14.7%). HEV-B was detected most frequently, with an overall prevalence of 47.1%. HEV-A and HEV-C were found in 32.3% and 20.6% of the samples, respectively. No HEV-D was identified. Molecular phylogeny indicated that all EV71 strains were of C4 genotype.
  
  
  CONCLUSIONS
  Although a variety of HEVs was detected in healthy children, HEV-B was relatively more prevalent than other HEV species. Considering HEV-A is more prevalent than HEV-B among patients with hand-foot-mouth disease, additional long-term surveillance of HEV is warranted in both asymptomatic and symptomatic populations.
authors.affiliation: MOE Key Laboratory of Environment & Health, Institute of Environmental Medicine, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wu
authors.forename: Wen-Bo
authors.initials: WB
authors.lastname: Xu
authors.forename: Long
authors.initials: L
authors.lastname: Chen
authors.forename: Hui-Ling
authors.initials: HL
authors.lastname: Chen
authors.forename: Qu
authors.initials: Q
authors.lastname: Liu
authors.forename: Dong-Li
authors.initials: DL
authors.lastname: Wang
authors.forename: Ying-Jian
authors.initials: YJ
authors.lastname: Chen
authors.forename: Wei
authors.initials: W
authors.lastname: Yao
authors.forename: Gang
authors.initials: G
authors.lastname: Li
authors.forename: Bin
authors.initials: B
authors.lastname: Feng
authors.forename: Bai-Hua
authors.initials: BH
authors.lastname: Shu
authors.forename: Yi-Kai
authors.initials: YK
authors.lastname: Zhou
authors.forename: Ya-Qing
authors.initials: YQ
authors.lastname: He
mesh_terms: D058070:Asymptomatic Diseases; D002675:Child, Preschool; D002681:China / Q000453:epidemiology; D029821:Enterovirus A, Human / Q000235:genetics* / Q000302:isolation & purification; D029822:Enterovirus B, Human / Q000235:genetics* / Q000302:isolation & purification; D030015:Enterovirus C, Human / Q000235:genetics* / Q000302:isolation & purification; D004769:Enterovirus Infections / Q000175:diagnosis / Q000453:epidemiology / Q000821:virology*; D005260:Female; D006801:Humans; D008297:Male; D012367:RNA, Viral / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D012367:RNA, Viral