title: Evaluation of bison (Bison bison) semen from Yellowstone National Park, Montana, USA, bulls for Brucella abortus shedding.
abstract: To determine if bison (Bison bison) bulls from Yellowstone National Park (YNP), Montana, USA, shed an infective dose of Brucella abortus in semen, 50 YNP bulls were captured on public lands in Montana during the winter and early spring (April-May) of 2010 and 2011. The bulls were immobilized, and blood and semen samples were collected for serology and Brucella culture. Thirty-five bulls (70%) were antibody-positive, and B. abortus was cultured from semen in three (9%) of the 35 antibody-positive or suspect bulls, though not at concentrations considered an infective dose. Eight bulls (six antibody-positive, two negative) had palpable lesions of the testes, epididymides, or seminal vesicles consistent with B. abortus infection. Breeding soundness exams and semen analysis suggested that antibody-positive bulls were more likely to have nonviable ejaculate (8/35; 23%) than bulls without detectable antibody (2/15; 13%).
authors.affiliation: United States Department of Agriculture, Animal and Plant Health Inspection Service, Veterinary Services (VS) Western Region, 2150 Centre Ave., Building B, Fort Collins, Colorado 80526, USA. rebecca.k.frey@aphis.usda.gov
authors.forename: Rebecca K
authors.initials: RK
authors.lastname: Frey
authors.forename: P Ryan
authors.initials: PR
authors.lastname: Clarke
authors.forename: Matt P
authors.initials: MP
authors.lastname: McCollum
authors.forename: Pauline
authors.initials: P
authors.lastname: Nol
authors.forename: Kammy R
authors.initials: KR
authors.lastname: Johnson
authors.forename: Brent D
authors.initials: BD
authors.lastname: Thompson
authors.forename: Jennifer M
authors.initials: JM
authors.lastname: Ramsey
authors.forename: Neil J
authors.initials: NJ
authors.lastname: Anderson
authors.forename: Jack C
authors.initials: JC
authors.lastname: Rhyan
mesh_terms: D000818:Animals; D000835:Animals, Wild / Q000382:microbiology; D000907:Antibodies, Bacterial / Q000097:blood*; D016164:Bison / Q000382:microbiology*; D002003:Brucella abortus / Q000276:immunology / Q000302:isolation & purification; D002006:Brucellosis / Q000453:epidemiology / Q000662:veterinary*; D008297:Male; D009009:Montana / Q000453:epidemiology; D012098:Reproduction / Q000502:physiology; D012661:Semen / Q000382:microbiology*; D016036:Seroepidemiologic Studies
chemical_list: D000907:Antibodies, Bacterial
keywords: Bison; brucellosis; culture; ejaculation; semen; serology