title: Recombinant adenovirus expressing ICP47 gene suppresses the ability of dendritic cells by restricting specific T cell responses.
abstract: Adenoviral vectors have been demonstrated to be one of the most effective vehicles to deliver foreign DNA into dendritic cells (DCs). However, the response of host immune systems against foreign gene products is a major obstacle to successful gene therapy. Infected cell protein 47 (ICP47) inhibits MHC Ⅰ antigen presentation pathway by binding to host transporter associated with antigen presentation (TAP), and thereby attenuates of specific cytotoxic T lymphocytes (CTLs) responses and evades the host immune clearance. This subject was designed to construct a recombinant adenovirus expressing His-tag-ICP47 fusion protein to investigate further the role of ICP47 in the elimination of transgene expression. Consequently, a recombinant adenovirus expressing the His-tag-ICP47 fusion protein was successfully constructed and it had the abilities of attenuating the stimulatory capacity of DCs by reducing the proliferation of lymphocytes and cytokine production of perforin compared with those of the r-track group and the control group. Our observations provide the first evidence of the regulation mechanism of ICP47 on DC-based immunotherapy for long-term persistence.
authors.affiliation: Nursing College, Zhengzhou University, Zhengzhou, Henan 450052, China. upliz@zzu.edu.cn
authors.forename: Peng
authors.initials: P
authors.lastname: Wang
authors.forename: Quancheng
authors.initials: Q
authors.lastname: Kan
authors.forename: Zujiang
authors.initials: Z
authors.lastname: Yu
authors.forename: Ling
authors.initials: L
authors.lastname: Li
authors.forename: Zhenxiang
authors.initials: Z
authors.lastname: Zhang
authors.forename: Xue
authors.initials: X
authors.lastname: Pan
authors.forename: Ting
authors.initials: T
authors.lastname: Feng
mesh_terms: D000256:Adenoviridae / Q000235:genetics; D015153:Blotting, Western; D049109:Cell Proliferation; D016207:Cytokines / Q000276:immunology / Q000378:metabolism; D003713:Dendritic Cells / Q000276:immunology* / Q000378:metabolism; D005434:Flow Cytometry; D005822:Genetic Vectors / Q000235:genetics; D006801:Humans; D017874:Immediate-Early Proteins / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D008213:Lymphocyte Activation / Q000276:immunology; D007959:Lymphocyte Culture Test, Mixed; D008214:Lymphocytes / Q000276:immunology / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology* / Q000378:metabolism; D019076:Transgenes / Q000235:genetics / Q000276:immunology; D014764:Viral Proteins / Q000235:genetics / Q000276:immunology* / Q000378:metabolism
chemical_list: D016207:Cytokines; C105843:ICP47 protein, Herpes simplex virus; D017874:Immediate-Early Proteins; D011993:Recombinant Fusion Proteins; D014764:Viral Proteins
keywords: Dendritic cells; ICP47; Recombinant adenovirus; Stimulatory capacity