title: Anti-nociceptive properties in rodents and the possibility of using polyphenol-rich fractions from sida urens L. (Malvaceae) against of dental caries bacteria.
abstract: BACKGROUND
  Sida urens L. (Malvaceae) is in flora of Asian medicinal herbs and used traditionally in West of Burkina Faso for the treatment of infectious diseases and particularly used against, dental caries bacteria, fever, pain and possesses analgesic properties. This study was conducted to reveal the antibacterial effect against dental caries bacteria on the one hand, and evaluate their analgesic capacity in experimental model with Swiss mice and on the other hand, with an aim to provide a scientific basis for the traditional use of this plant for the management of dental caries bacteria.
  
  
  METHODS
  The antibacterial assays in this study were performed by using inhibition zone diameters, MIC (Minimum inhibitory concentration) and MBC (Minimal bactericidal concentration) methods. On the whole the dental caries bacteria (Gram-positive and Gram-negative bacterial strains) were used. Negative control was prepared using discs impregnated with 10% DMSO in water and commercially available Gentamicin from Alkom Laboratories LTD was used as positive reference standards for all bacterial strains. In acute toxicity test, mice received doses of extract (acetone/water extract) from Sida urens L. by intraperitoneal route and LD50 was determined in Swiss mice. As for analgesic effects, acetic acid writhing method was used in mice. The acetic acid-induced writhing method was used in mice with aim to study analgesic effects.
  
  
  RESULTS
  The results showed that the highest antibacterial activities were founded with the polyphenol-rich fractions against all bacterial strains compared to the standard antibiotic. About preliminary study in acute toxicity test, LD50 value obtained was more than 5000 mg/kg b.w. Polyphenol-rich fractions produced significant analgesic effects in acetic acid-induced writhing method and in a dose-dependent inhibition was observed.
  
  
  CONCLUSIONS
  These results validate the ethno-botanical use of Sida urens L. (Malvaceae) and demonstrate the potential of this herbaceous as a potential antibacterial agent of dental caries that could be effectively used for future health care purposes.
authors.forename: Kiessoun
authors.initials: K
authors.lastname: Konaté
authors.forename: Patrice
authors.initials: P
authors.lastname: Zerbo
authors.forename: Maurice
authors.initials: M
authors.lastname: Ouédraogo
authors.forename: Crépin I
authors.initials: CI
authors.lastname: Dibala
authors.forename: Hilou
authors.initials: H
authors.lastname: Adama
authors.forename: Oksana
authors.initials: O
authors.lastname: Sytar
authors.forename: Marian
authors.initials: M
authors.lastname: Brestic
authors.forename: Nicolas
authors.initials: N
authors.lastname: Barro
mesh_terms: D000700:Analgesics / Q000302:isolation & purification / Q000494:pharmacology* / Q000633:toxicity; D000818:Animals; D000900:Anti-Bacterial Agents / Q000302:isolation & purification / Q000494:pharmacology* / Q000633:toxicity; D004305:Dose-Response Relationship, Drug; D005260:Female; D006090:Gram-Negative Bacteria / Q000187:drug effects; D006094:Gram-Positive Bacteria / Q000187:drug effects; D007928:Lethal Dose 50; D008297:Male; D019660:Malvaceae / Q000737:chemistry*; D051379:Mice; D008826:Microbial Sensitivity Tests; D009619:Nociceptors / Q000187:drug effects; D010147:Pain Measurement; D010946:Plants, Medicinal / Q000737:chemistry; D059808:Polyphenols / Q000302:isolation & purification / Q000494:pharmacology* / Q000633:toxicity
chemical_list: D000700:Analgesics; D000900:Anti-Bacterial Agents; D059808:Polyphenols