title: Aging affects high-density lipoprotein composition and function.
abstract: Most coronary deaths occur in patients older than 65years. Age associated alterations in the composition and function of high-density lipoproteins (HDL) may contribute to cardiovascular mortality. The effect of advanced age on the composition and function of HDL is not well understood. HDL was isolated from healthy young and elderly subjects. HDL composition, cellular cholesterol efflux/uptake, anti-oxidant properties and paraoxonase activity were assessed. We observed a 3-fold increase of the acute phase protein serum amyloid A, an increased content of complement C3 and proteins involved in endopeptidase/protease inhibition in HDL of elderly subjects, whereas levels of apolipoprotein E were significantly decreased. HDL from elderly subjects contained less cholesterol but increased sphingomyelin. Most importantly, HDL from elderly subjects showed defective antioxidant properties, lower paraoxonase 1 activity and was more rapidly taken up by macrophages, whereas cholesterol efflux capability was not altered. These findings suggest that aging alters HDL composition, resulting in functional impairment that may contribute to the onset/progression of cardiovascular disease.
authors.affiliation: Institute of Experimental and Clinical Pharmacology, Medical University of Graz, Austria.
authors.forename: Michael
authors.initials: M
authors.lastname: Holzer
authors.forename: Markus
authors.initials: M
authors.lastname: Trieb
authors.forename: Viktoria
authors.initials: V
authors.lastname: Konya
authors.forename: Christian
authors.initials: C
authors.lastname: Wadsack
authors.forename: Akos
authors.initials: A
authors.lastname: Heinemann
authors.forename: Gunther
authors.initials: G
authors.lastname: Marsche
mesh_terms: D000328:Adult; D000368:Aged; D000375:Aging / Q000378:metabolism / Q000473:pathology*; D000975:Antioxidants / Q000378:metabolism; D043303:Aryldialkylphosphatase / Q000378:metabolism; D002265:Carboxylic Ester Hydrolases / Q000378:metabolism; D002784:Cholesterol / Q000378:metabolism; D005260:Female; D006801:Humans; D008075:Lipoproteins, HDL / Q000097:blood / Q000737:chemistry*; D008264:Macrophages / Q000378:metabolism; D008297:Male; D000685:Serum Amyloid A Protein / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D000975:Antioxidants; D008075:Lipoproteins, HDL; D000685:Serum Amyloid A Protein; D002784:Cholesterol; D002265:Carboxylic Ester Hydrolases; C019734:arylesterase; D043303:Aryldialkylphosphatase
keywords: Anti-oxidative activity; Cardiovascular disease; Paraoxonase; Proteome