title: Identification of G protein-coupled receptors in the pea aphid, Acyrthosiphon pisum.
abstract: GPCRs play crucial roles in the growth, development and reproduction of organisms. In insects, a large number of GPCRs have been reported for Holometabola but not Hemimetabola. The recently sequenced pea aphid genome provides us with the opportunity to analyze the evolution and potential functions of GPCRs in Hemimetabola. 82 GPCRs were identified from the representative model hemimetabolous insect Acyrthosiphon pisum, 37 of which have ESTs evidence, and 73 are annotated for the first time. A striking difference between A. pisum, Drosophila melanogaster and Tribolium castaneum is the duplication of the kinin and SIFamide receptors in A. pisum. Another divergence is the loss of the sulfakinin receptor in A. pisum. These duplications/losses are likely involved in the osmoregulation, reproduction and energy metabolism of A. pisum. Moreover, this work will promote functional analyses of GPCRs in A. pisum and may advance new drug target discovery for biological control of the aphid.
authors.affiliation: Jiangsu Key Laboratory for Biodiversity and Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing 210023, China. Electronic address: lcj3314@163.com.
authors.forename: Chengjun
authors.initials: C
authors.lastname: Li
authors.forename: Xiaopei
authors.initials: X
authors.lastname: Yun
authors.forename: Xingxing
authors.initials: X
authors.lastname: Hu
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhang
authors.forename: Ming
authors.initials: M
authors.lastname: Sang
authors.forename: Xing
authors.initials: X
authors.lastname: Liu
authors.forename: Wei
authors.initials: W
authors.lastname: Wu
authors.forename: Bin
authors.initials: B
authors.lastname: Li
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001042:Aphids / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D004331:Drosophila melanogaster / Q000235:genetics / Q000378:metabolism; D019143:Evolution, Molecular; D020224:Expressed Sequence Tags; D020440:Gene Duplication; D015964:Gene Expression Regulation, Bacterial; D019476:Insect Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D018532:Pisum sativum / Q000469:parasitology*; D010802:Phylogeny; D043562:Receptors, G-Protein-Coupled / Q000235:genetics* / Q000378:metabolism; D014231:Tribolium / Q000235:genetics / Q000378:metabolism
chemical_list: D019476:Insect Proteins; D043562:Receptors, G-Protein-Coupled
keywords: 7 transmembrane; 7TM; ACP; AKH; AKH/corazonin-related peptide; AVPL; Acyrthosiphon pisum; Biogenic amine; Boss; CCAP; ESTs; ETH; G protein-coupled receptors; GABA-B; GPA2; GPB5; GPCR; GPCRs; HE6; Kinin; Neuropeptide; SIFamide; Stan; Sulfakinin; Trissin; adipokinetic hormone; arginine vasopressin-like peptide; bride of sevenless; crustacean cardioactive peptide; ecdysis triggering hormone; expressed sequence tags; gamma-amino butyric acid B; glycoprotein hormone alpha2; glycoprotein hormone beta5; human epididymis 6; starry night