title: The Pho regulon influences biofilm formation and type three secretion in Pseudomonas aeruginosa.
abstract: Research into the molecular mechanisms of the switch from highly motile to biofilm forming Pseudomonas fluorescens bacteria recently uncovered a role of inorganic phosphate as an important environmental regulatory factor to control c-di-GMP levels in the cell. In this study we present evidence that in the opportunistic pathogen P. aeruginosa the Pho regulon inhibits biofilm formation and is required for the repression of the type three secretion system. We furthermore identified an EAL domain protein as a downstream effector of the Pho regulon, which at least partially mediated the observed inhibition. Interestingly, inhibition of the P. aeruginosa virulence phenotype was Pho regulon-dependent in both a PA14 and a PAO1 strain background; however, in PA14 this inhibition was independent on the availability of inorganic phosphate, whereas in PAO1 phosphate enhanced biofilm formation independently of the inhibitory activity of the Pho regulon. These results clearly show that the Pho regulon contributes to the expression of the virulence phenotype in P. aeruginosa and add even more complexity to the strain-specific regulation of bacterial behaviour by environmental cues.
authors.affiliation: Chronic Pseudomonas Infection Research Group, Helmholtz Center for Infection Research (HZI), Inhoffenstrasse 7, 38124 Braunschweig, Germany. TWINCORE, Center for Experimental and Clinical Infection Research, a joint venture of the HZI and the Hannover Medical School, Feodor-Lynen-Strasse 7, 30625 Hannover, Germany.
authors.forename: Ahmed
authors.initials: A
authors.lastname: Haddad
authors.forename: Vanessa
authors.initials: V
authors.lastname: Jensen
authors.forename: Tanja
authors.initials: T
authors.lastname: Becker
authors.forename: Susanne
authors.initials: S
authors.lastname: Häussler