title: Involvement of reactive oxygen species in osteoblastic differentiation of MC3T3-E1 cells accompanied by mitochondrial morphological dynamics.
abstract: Bone remodeling is regulated by local factors that regulate bone-forming osteoblasts and bone-resorbing osteoclasts, in addition to hormonal activity. Recent studies have shown that reactive oxygen species (ROS) act as an intracellular signal mediator for osteoclast differentiation. However the role of ROS on osteoblast differentiation is poorly understood. Here, we investigated the impact of ROS on osteoblastic differentiation of MC3T3-E1 cells. Osteogenic induction resulted in notable enhancement of mineralization and expression of osteogenic marker gene alkaline phosphatase, which were accompanied by an increase in ROS production. Additionally, we found that mitochondrial morphology dynamically changed from tubular reticulum to fragmented structures during the differentiation, suggesting that mitochondrial morphological transition is a novel osteoblast differentiation index. The antioxidant N-acetyl cysteine prevented not only ROS production but also mineralization and mitochondrial fragmentation. It is therefore suggested that the ROS-dependent signaling pathways play a role in osteoblast differentiation accompanied by mitochondrial morphological transition.
authors.affiliation: Department of Molecular Cell Biology and Medicine, Institute of Health Biosciences, The University of Tokushima Graduate School, Tokushima 770-8505, Japan. narakaki@tokushima-u.ac.jp
authors.forename: Naokatu
authors.initials: N
authors.lastname: Arakaki
authors.forename: Arisa
authors.initials: A
authors.lastname: Yamashita
authors.forename: Shingo
authors.initials: S
authors.lastname: Niimi
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Yamazaki
mesh_terms: D000111:Acetylcysteine / Q000494:pharmacology; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D015415:Biomarkers / Q000378:metabolism; D002113:Calcification, Physiologic / Q000502:physiology; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D051379:Mice; D008928:Mitochondria / Q000187:drug effects / Q000201:enzymology / Q000648:ultrastructure*; D063154:Mitochondrial Dynamics / Q000187:drug effects; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects / Q000201:enzymology; D010012:Osteogenesis / Q000502:physiology; D017382:Reactive Oxygen Species / Q000037:antagonists & inhibitors / Q000378:metabolism*
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D017382:Reactive Oxygen Species; D000469:Alkaline Phosphatase; D000111:Acetylcysteine