title: Developmental hypothyroxinaemia induced by maternal mild iodine deficiency delays hippocampal axonal growth in the rat offspring.
abstract: Iodine is essential for the biosynthesis of thyroid hormones, including triiodothyronine and thyroxine. Thyroid hormones are important for central nervous system development. Mild maternal iodine deficiency (ID)-induced hypothyroxinaemia causes neurological deficits and mental retardation of the foetus. However, the detailed mechanism underlying these deficits is still largely unknown. Given that the growth-associated protein of 43 kDa (GAP-43), semaphorin 3A (Sema3A) and the glycogen synthase kinase 3β (GSK3β)/collapsin response mediator protein 2 (CRMP2) pathway are essential for axonal development, we hypothesise that hippocampal axonal growth-related proteins may be impaired, which may contribute to hippocampal axonal growth delay in rat offspring exposed to maternal hypothyroxinaemia. To test this hypothesis, maternal hypothyroxinaemia models were established in Wistar rats using a mild ID diet. Besides a negative control group, two maternal hypothyroidism models were created with either a severe ID diet or methimazole in the water. Our results showed that maternal hypothyroxinaemia exposure delayed offspring axonal growth on gestational day 19, postnatal day (PN) 7, PN14 and PN21. Consistent with this, the mean intensity of hippocampal CRMP2 and Tau1 immunofluorescence axonal protein was reduced in the mild ID group. Moreover, maternal hypothyroxinaemia disrupted expressions of GAP-43 and Sema3A. Furthermore, the phosphorylation of GSK3β and CRMP2 was also affected in the treated offspring, implying a potential mechanism by which hypothyroxinaemia-exposure affects neurodevelopment. Taken together, our data support the hypothesis that maternal hypothyroxinaemia may impair axonal growth of the offspring.
authors.affiliation: Department of Occupational and Environmental Health, School of Public Health, China Medical University, Shenyang, China.
authors.forename: W
authors.initials: W
authors.lastname: Wei
authors.forename: Y
authors.initials: Y
authors.lastname: Wang
authors.forename: Y
authors.initials: Y
authors.lastname: Wang
authors.forename: J
authors.initials: J
authors.lastname: Dong
authors.forename: H
authors.initials: H
authors.lastname: Min
authors.forename: B
authors.initials: B
authors.lastname: Song
authors.forename: W
authors.initials: W
authors.lastname: Teng
authors.forename: Q
authors.initials: Q
authors.lastname: Xi
authors.forename: J
authors.initials: J
authors.lastname: Chen
mesh_terms: D000818:Animals; D001369:Axons / Q000502:physiology*; D049108:Cell Enlargement; D005260:Female; D019922:GAP-43 Protein / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental / Q000502:physiology; D038362:Glycogen Synthase Kinase 3 / Q000378:metabolism; D000071679:Glycogen Synthase Kinase 3 beta; D006624:Hippocampus / Q000166:cytology* / Q000254:growth & development; D007037:Hypothyroidism / Q000139:chemically induced / Q000378:metabolism / Q000503:physiopathology*; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism; D007455:Iodine / Q000172:deficiency* / Q000502:physiology; D008297:Male; D008713:Methimazole; D009419:Nerve Tissue Proteins / Q000378:metabolism; D010766:Phosphorylation; D011247:Pregnancy; D051381:Rats; D040121:Semaphorin-3A / Q000378:metabolism; D016875:tau Proteins / Q000378:metabolism
chemical_list: D019922:GAP-43 Protein; D036341:Intercellular Signaling Peptides and Proteins; D009419:Nerve Tissue Proteins; C488218:Sema3a protein, rat; D040121:Semaphorin-3A; C095114:collapsin response mediator protein-2; D016875:tau Proteins; D008713:Methimazole; D007455:Iodine; D000071679:Glycogen Synthase Kinase 3 beta; C000605508:Gsk3b protein, rat; D038362:Glycogen Synthase Kinase 3
keywords: axon; hippocampus; hypothyroidism; hypothyroxinaemia; iodine deficiency