title: Detection and characterization of a divergent avian reovirus strain from a broiler chicken with central nervous system disease.
abstract: Avian orthoreoviruses have been associated with a variety of diseases in chickens, including tenosynovitis, runting-stunting syndrome, hepatitis, myocarditis, osteoporosis, respiratory diseases, and central nervous system disease. The primary objective of our study was the molecular characterization of an avian reovirus strain, T1781, which was isolated from a broiler chicken with a central nervous system disorder in Hungary during 2012. The complete genome sequence was determined using a traditional sequencing method after cell culture adaptation of the strain. Sequence and phylogenetic analyses showed that T1781 shared only moderate nucleic acid sequence identity in several genes to previously analyzed reovirus strains from chickens, and each gene formed separate branches in the corresponding phylogenetic trees. The maximum nucleotide sequence identities of strain T1781 genes to reference avian reovirus strains ranged from 79 % to 90 %. Collectively, our analyses indicated that T1781 is a divergent chicken reovirus strain. The genetic background of this and other avian reoviruses associated with various disease manifestations needs further investigation.
authors.affiliation: Institute for Veterinary Medical Research, Centre of Agricultural Research, Hungarian Academy of Sciences, Budapest, P.O. Box 18, 1581, Hungary.
authors.forename: Eszter
authors.initials: E
authors.lastname: Dandár
authors.forename: Adám
authors.initials: A
authors.lastname: Bálint
authors.forename: Sándor
authors.initials: S
authors.lastname: Kecskeméti
authors.forename: Katalin
authors.initials: K
authors.lastname: Szentpáli-Gavallér
authors.forename: Péter
authors.initials: P
authors.lastname: Kisfali
authors.forename: Béla
authors.initials: B
authors.lastname: Melegh
authors.forename: Szilvia L
authors.initials: SL
authors.lastname: Farkas
authors.forename: Krisztián
authors.initials: K
authors.lastname: Bányai
mesh_terms: D000818:Animals; D002494:Central Nervous System Infections / Q000662:veterinary* / Q000821:virology; D002645:Chickens; D016000:Cluster Analysis; D016679:Genome, Viral; D006814:Hungary; D030101:Orthoreovirus, Avian / Q000235:genetics* / Q000302:isolation & purification; D010802:Phylogeny; D011201:Poultry Diseases / Q000821:virology*; D012367:RNA, Viral / Q000235:genetics*; D012088:Reoviridae Infections / Q000662:veterinary* / Q000821:virology; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid
chemical_list: D012367:RNA, Viral