title: Disentangling two QTL on porcine chromosome 12 for backfat fatty acid composition.
abstract: A previous study allowed the identification of two QTL regions at positions 11-34 cM (QTL1) and 68-76 cM (QTL2) on porcine chromosome SSC12 affecting several backfat fatty acids in an Iberian x Landrace F2 intercross. In the current study, different approaches were performed in order to better delimit the quoted QTL regions and analyze candidate genes. A new chromosome scan, using 81 SNPs selected from the Porcine 60KBeadChip and six previously genotyped microsatellites have refined the QTL positions. Three new functional candidate genes (ACOX1, ACLY, and SREBF1) have been characterized. Moreover, two putative promoters of porcine ACACA gene have also been investigated. New isoforms and 24 SNPs were detected in the four candidate genes, 19 of which were genotyped in the population. ACOX1 and ACLY SNPs failed to explain the effects of QTL1 on palmitic and gadoleic fatty acids. QTL2, affecting palmitoleic, stearic, and vaccenic fatty acids, maps close to the ACACA gene location. The most significant associations have been detected between one intronic (g.53840T > C) and one synonymous (c.5634T > C) ACACA SNPs and these fatty acids. Complementary analyses including ACACA gene expression quantification and association studies in other porcine genetic types do not support the expected causal effect of ACACA SNPs.
authors.affiliation: Departamento de Mejora Genética Animal, INIA, Madrid, Spain. mariamm@inia.es
authors.forename: María
authors.initials: M
authors.lastname: Muñoz
authors.forename: Ana Isabel
authors.initials: AI
authors.lastname: Fernández
authors.forename: Rita
authors.initials: R
authors.lastname: Benítez
authors.forename: Ramona N
authors.initials: RN
authors.lastname: Pena
authors.forename: Josep María
authors.initials: JM
authors.lastname: Folch
authors.forename: María del Carmen
authors.initials: Mdel C
authors.lastname: Rodríguez
authors.forename: Luis
authors.initials: L
authors.lastname: Silió
authors.forename: Estefânia
authors.initials: E
authors.lastname: Alves
mesh_terms: D000103:Acetyl-CoA Carboxylase / Q000235:genetics; D000273:Adipose Tissue / Q000737:chemistry / Q000378:metabolism*; D000818:Animals; D001415:Back; D033481:Chromosomes, Mammalian; D005227:Fatty Acids / Q000032:analysis*; D005260:Female; D008040:Genetic Linkage; D050356:Lipid Metabolism / Q000235:genetics*; D008297:Male; D011110:Polymorphism, Genetic; D040641:Quantitative Trait Loci; D051780:Sterol Regulatory Element Binding Protein 1 / Q000235:genetics; D013552:Swine / Q000235:genetics*
chemical_list: D005227:Fatty Acids; D051780:Sterol Regulatory Element Binding Protein 1; D000103:Acetyl-CoA Carboxylase