title: Polyamidoamine dendrimers-capped carbon dots/Au nanocrystal nanocomposites and its application for electrochemical immunosensor.
abstract: In this work, polyamidoamine dendrimers capped-carbon dots (PAMAM-CDs) were fabricated by one-step microwave assisted pyrolysis of citric acid (CA) and PAMAM, where the formation of CDs and the surface passivation were accomplished simultaneously. The obtained graphitic PAMAM-CDs, with abundant amine groups, were employed as reducing and capping agents for the formation of PAMAM-CDs/Au nanocrystal nanocomposites. The resulting nanocomposites exhibited excellent conductivity, stability and biocompatibility on the surface of electrode and were designed as an immobilized matrix for sensitive immunosensing of alpha-fetoprotein (AFP). The proposed immunosensor showed a wide linear detection range from 100 fg mL(-1) to 100 ng mL(-1). The detection limit for AFP was 0.025 pg mL(-1). Importantly, the immunosensor was evaluated for the analysis of clinical serum samples, obtaining a good correlation with enzyme-linked immunosorbent assay (ELISA). The results indicated that the immunosensor provided a possible application for the detection of AFP in clinical diagnosis.
authors.affiliation: Department of Chemistry, Capital Normal University, Beijing 100048, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Gao
authors.forename: Jingman
authors.initials: J
authors.lastname: Han
authors.forename: Zhanfang
authors.initials: Z
authors.lastname: Ma
mesh_terms: D015374:Biosensing Techniques / Q000379:methods; D050091:Dendrimers / Q000737:chemistry*; D055664:Electrochemical Techniques / Q000379:methods*; D004566:Electrodes; D006046:Gold / Q000737:chemistry*; D006801:Humans; D007118:Immunoassay / Q000379:methods; D057230:Limit of Detection; D053761:Nanocomposites / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry*; D011073:Polyamines / Q000737:chemistry*; D000509:alpha-Fetoproteins / Q000032:analysis*
chemical_list: D050091:Dendrimers; C531249:Poly(amidoamine); D011073:Polyamines; D000509:alpha-Fetoproteins; D006046:Gold
keywords: Alpha-fetoprotein; Au nanocrystal; Carbon dots; Label-free electrochemical immunosensor; Polyamidoamine dendrimers