title: IR-780 dye loaded tumor targeting theranostic nanoparticles for NIR imaging and photothermal therapy.
abstract: IR-780 iodide is a near-infrared (NIR) fluorescence dye with higher and more stable fluorescence intensity than clinically applied dye indocyanine green (ICG). Meanwhile, IR-780 can be utilized in photothermal therapy with laser irradiation. IR-780 is an important theranostic agent but its lipophilicity limited its application. In this paper, we synthesize multifunctional heparin-folic acid-IR-780 nanoparticles (HF-IR-780 NPs) by self-assembly of the heparin-folic acid conjugate and IR-780 through ultrasonic sound method. The HF-IR-780 NPs exhibit good monodispersity, significant stability, and excellent molecular targeting to folate receptor over-expressing MCF-7 cells. Furthermore, the in vivo biodistribution experiments show that the HF-IR-780 NPs are specifically targeted to the tumor and can be used for tumor imaging. The in vitro cell viability assays and in vivo photothermal therapy experiments indicate that MCF-7 cells or MCF-7 xenograft tumors could be ablated by combining HF-IR-780 NPs with irradiation of an 808 nm laser. The photothermal therapy in vivo with a single-dose treatment has not caused significant adverse effect. The resulted HF-IR-780 NPs are a potential theranostic agent for imaging-guided cancer therapy.
authors.affiliation: Guangdong Key Laboratory of Nanomedicine, Institute of Biomedicine and Biotechnology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, PR China.
authors.forename: Caixia
authors.initials: C
authors.lastname: Yue
authors.forename: Peng
authors.initials: P
authors.lastname: Liu
authors.forename: Mingbin
authors.initials: M
authors.lastname: Zheng
authors.forename: Pengfei
authors.initials: P
authors.lastname: Zhao
authors.forename: Yiqing
authors.initials: Y
authors.lastname: Wang
authors.forename: Yifan
authors.initials: Y
authors.lastname: Ma
authors.forename: Lintao
authors.initials: L
authors.lastname: Cai
mesh_terms: D000818:Animals; D001943:Breast Neoplasms / Q000175:diagnosis / Q000628:therapy; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000502:physiology; D003952:Diagnostic Imaging / Q000379:methods*; D016503:Drug Delivery Systems; D005260:Female; D006801:Humans; D007211:Indoles / Q000737:chemistry*; D051379:Mice; D008819:Mice, Nude; D053758:Nanoparticles / Q000737:chemistry* / Q000627:therapeutic use; D010789:Phototherapy / Q000379:methods*
chemical_list: C548458:2-(2-(2-chloro-3-((1,3-dihydro-3,3-dimethyl-1-propyl-2H-indol-2-ylidene)ethylidene)-1-cyclohexen-1-yl)ethenyl)-3,3-dimethyl-1-propylindolium; D007211:Indoles