title: Proteomic analysis of rat tibialis anterior muscles at different stages of experimental autoimmune myasthenia gravis.
abstract: Myasthenia gravis (MG) is an autoimmune disease in which autoantibodies, most commonly directed against the acetylcholine receptor (AChR), impair neuromuscular transmission and cause muscle weakness. In this study, we utilized two-dimensional difference in-gel electrophoresis (2D-DIGE) to analyze the muscle's proteomic profile at different stages of experimental autoimmune myasthenia gravis (EAMG). We identified twenty-two differentially expressed proteins, mainly related to metabolic and stress-response pathways. Interestingly, these identified proteins have also been associated with other contraction-impairing muscle pathologies (e.g. inclusion body myositis), suggesting a similar response of the muscle to such conditions.
authors.affiliation: Division Neuroscience, School for Mental Health and Neuroscience, Maastricht University, 6200 MD Maastricht, the Netherlands.
authors.forename: Alejandro M
authors.initials: AM
authors.lastname: Gomez
authors.forename: Annelies
authors.initials: A
authors.lastname: Vanheel
authors.forename: Mario
authors.initials: M
authors.lastname: Losen
authors.forename: Peter C
authors.initials: PC
authors.lastname: Molenaar
authors.forename: Marc H
authors.initials: MH
authors.lastname: De Baets
authors.forename: Jean-Paul
authors.initials: JP
authors.lastname: Noben
authors.forename: Niels
authors.initials: N
authors.lastname: Hellings
authors.forename: Pilar
authors.initials: P
authors.lastname: Martinez-Martinez
mesh_terms: D000818:Animals; D018450:Disease Progression; D005260:Female; D018482:Muscle, Skeletal / Q000378:metabolism* / Q000473:pathology*; D020720:Myasthenia Gravis, Autoimmune, Experimental / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D040901:Proteomics / Q000379:methods*; D051381:Rats; D011917:Rats, Inbred Lew
keywords: 2D-DIGE; Autoimmunity; Muscle proteomics; Myasthenia gravis