title: Characterization of a feruloyl esterase from Lactobacillus plantarum.
abstract: Lactobacillus plantarum is frequently found in the fermentation of plant-derived food products, where hydroxycinnamoyl esters are abundant. L. plantarum WCFS1 cultures were unable to hydrolyze hydroxycinnamoyl esters; however, cell extracts from the strain partially hydrolyze methyl ferulate and methyl p-coumarate. In order to discover whether the protein Lp_0796 is the enzyme responsible for this hydrolytic activity, it was recombinantly overproduced and enzymatically characterized. Lp_0796 is an esterase that, among other substrates, is able to efficiently hydrolyze the four model substrates for feruloyl esterases (methyl ferulate, methyl caffeate, methyl p-coumarate, and methyl sinapinate). A screening test for the detection of the gene encoding feruloyl esterase Lp_0796 revealed that it is generally present among L. plantarum strains. The present study constitutes the description of feruloyl esterase activity in L. plantarum and provides new insights into the metabolism of hydroxycinnamic compounds in this bacterial species.
authors.affiliation: Laboratorio de Biotecnología Bacteriana, Instituto de Ciencia y Tecnología de Alimentos y Nutrición (ICTAN-CSIC), Madrid, Spain.
authors.forename: María
authors.initials: M
authors.lastname: Esteban-Torres
authors.forename: Inés
authors.initials: I
authors.lastname: Reverón
authors.forename: José Miguel
authors.initials: JM
authors.lastname: Mancheño
authors.forename: Blanca
authors.initials: B
authors.lastname: de Las Rivas
authors.forename: Rosario
authors.initials: R
authors.lastname: Muñoz
mesh_terms: D002109:Caffeic Acids / Q000378:metabolism; D002265:Carboxylic Ester Hydrolases / Q000235:genetics / Q000378:metabolism*; D002934:Cinnamates / Q000378:metabolism; D015870:Gene Expression; D048191:Lactiplantibacillus plantarum / Q000201:enzymology* / Q000235:genetics; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D002109:Caffeic Acids; D002934:Cinnamates; D011994:Recombinant Proteins; C478851:methyl-p-coumarate; C006605:antithiamine factor; C042405:methyl caffeate; D002265:Carboxylic Ester Hydrolases; C070196:feruloyl esterase; C530806:methyl ferulate