title: Ex-vivo effect of intracanal medications based on ozone and calcium hydroxide in root canals contaminated with Enterococcus faecalis.
abstract: This ex vivo study evaluated the antibacterial effect of intracanal medications in root canals contaminated with Enterococcus faecalis. Fifty single-rooted human teeth were contaminated with E. faecalis (ATCC 29212) and incubated at 37°C for 21 days. The specimens were randomly divided into 5 groups according to the intracanal medication used: OZ-PG: ozonized propylene glycol; CH/CPMC: calcium hydroxide/camphorated paramonochlorophenol; OZ-PG/CH ozonized PG/CH; PC: positive control group (no medication); and NC: negative control group (no contamination). The samples were collected after 7 days (post-medication) and 14 days (final). Bacterial growth was checked by counting the colony-forming units (CFU). OZ-PG and CH/CPMC reduced significantly the CFU counts compared with PC in the post-medication and final samples, with no statistically significant differences among them. On the other hand, OZ-PG/CH did not reduce significantly the number of bacteria compared with PC. In conclusion, among the evaluated medications OZ-PG and CH/CPMC were the most effective against E. faecalis.
authors.affiliation: Department of Restorative Dentistry, Araraquara Dental School, UNESP - Univ Estadual Paulista, Araraquara, SP, Brazil.
authors.forename: Roberta Vieira
authors.initials: RV
authors.lastname: Farac
authors.forename: Antonio Carlos
authors.initials: AC
authors.lastname: Pizzolitto
authors.forename: Juliane Maria Guerreiro
authors.initials: JM
authors.lastname: Tanomaru
authors.forename: Renata Dornelles
authors.initials: RD
authors.lastname: Morgental
authors.forename: Regina Karla de Pontes
authors.initials: RK
authors.lastname: Lima
authors.forename: Idomeo
authors.initials: I
authors.lastname: Bonetti-Filho
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D058491:Bacterial Load / Q000187:drug effects; D001431:Bacteriological Techniques; D002126:Calcium Hydroxide / Q000494:pharmacology*; D002164:Camphor / Q000494:pharmacology; D002733:Chlorophenols / Q000494:pharmacology; D003786:Dental Pulp Cavity / Q000382:microbiology*; D004338:Drug Combinations; D013293:Enterococcus faecalis / Q000187:drug effects* / Q000254:growth & development; D006801:Humans; D010126:Ozone / Q000494:pharmacology*; D014677:Pharmaceutical Vehicles; D019946:Propylene Glycol; D012388:Root Canal Irrigants / Q000494:pharmacology*; D013997:Time Factors
chemical_list: D000900:Anti-Bacterial Agents; D002733:Chlorophenols; D004338:Drug Combinations; D014677:Pharmaceutical Vehicles; D012388:Root Canal Irrigants; D010126:Ozone; D019946:Propylene Glycol; D002164:Camphor; C029804:camphorated parachlorophenol; D002126:Calcium Hydroxide