title: Dynamic structural changes in microbial membranes in response to high hydrostatic pressure analyzed using time-resolved fluorescence anisotropy measurement.
abstract: High hydrostatic pressure has a profound physiological impact on lipid membranes, primarily resulting in tighter packing and restriction of acyl-chain motion. To fulfill membrane protein functions in high-pressure environments, deep-sea organisms possess specialized cell membranes. Although the effects of high-pressure on model membranes have been investigated in great detail, high-pressure-induced structural changes in living cell membranes remain to be elucidated. Of the spectroscopic techniques available to date, fluorescence anisotropy measurement is a common useful method that provides information on dynamic membrane properties. This mini-review focuses on pressure-induced changes in natural cell membranes, analyzed by means of high-pressure time-resolved fluorescence anisotropy measurement (HP-TRFAM). Specifically, the role of eicosapentaenoic acid in deep-sea piezophiles is described in terms of the structural integrity of the membrane under high pressure.
authors.affiliation: Department of Chemistry and Biological Science, College of Science and Engineering, Aoyama Gakuin University, Sagamihara, Japan; Institute of Biogeosciences, Japan Agency for Marine-Earth Science and Technology (JAMSTEC), Yokosuka, Japan. Electronic address: abef@chem.aoyama.ac.jp.
authors.forename: Fumiyoshi
authors.initials: F
authors.lastname: Abe
mesh_terms: D002462:Cell Membrane / Q000187:drug effects / Q000502:physiology*; D005454:Fluorescence Polarization; D006874:Hydrostatic Pressure; D008563:Membrane Lipids / Q000494:pharmacology; D008565:Membrane Proteins / Q000502:physiology*; D008567:Membranes, Artificial
chemical_list: D008563:Membrane Lipids; D008565:Membrane Proteins; D008567:Membranes, Artificial
keywords: 1,6-diphenyl-1,3,5-hexatriene; 1-[4-(trimethylamino)pheny]-6-phenyl-1,3,5-hexatriene; D(w); DHA; DOPC; DPH; DPPC; DSPC; Deep-sea piezophile; EPA; HP-TRFAM; High-pressure time-resolved fluorescence anisotropy measurement (HP-TRFAM); Membrane fluidity; POPC; PUFA; S; SAPC; SDPC; SOPC; TCSPC; TMA-DPH; dioleyl-phosphatidylcholine; dipalmitoyl-phosphatidylcholine; distearoyl-phosphatidylcholine; docosahexaenoic acid (C22:6); eicosapentaenoic acid (C20:5); fluorescence anisotropy; high-pressure time-resolved fluorescence anisotropy measurement; order parameter; palmitoyl-oleoyl-phosphatidylcholine; polyunsaturated fatty acids; r; rotational diffusion coefficient; stearoyl-arachidonoyl-phosphatidylcholine; stearoyl-docosahexanoylphosphatidylcholine; stearoyl-oleyl-phosphatidylcholine; time-correlated single-photon counting