title: Inhibitory effect of melanoma differentiation associated gene-7/interleukin-24 on invasion in vitro of human melanoma cancer cells.
abstract: The acquisition of metastasis potential is a critical point for malignant tumors. Melanoma differentiation associated gene-7/interleukin-24 (mda-7/IL-24) is a potential tumor suppress gene and frequently down-regulated in malignant tumors. It has been implicated that overexpression of MDA-7 led to proliferation inhibition in many types of human tumor. Invasion is an important process which is potential to promote tumor metastasis. However, the role and potential molecular mechanism of mda-7/IL-24 to inhibit the invasion of human melanoma cancer is not fully clear. In this report, we identified a solid role for mda-7/IL-24 in invasion inhibition of human melanoma cancer LiBr cells, including decreasing of adhesion and invasion in vitro, blocking cell cycle, down-regulating the expression of ICAM-1, MMP-2/9, CDK1, the phosphorylation of ERK and Akt, NF-κB and AP-1 transcription activity. Meanwhile, there was an increased expression of PTEN in mda-7/IL-24 over-expression LiBr cells. Our results demonstrated that mda-7/IL-24 is a potential invasion suppress gene, which inhibits the invasion of LiBr cells by the down-regulation of ICAM-1, MMP-2/9, PTEN, and CDK1 expression. The molecular pathways involved were the MAPK/ERK, PI3K-Akt, NF-κB, and AP-1. These findings suggest that mda-7/IL-24 may be used as a possible therapeutic strategy for human melanoma cancer.
authors.affiliation: Department of Dermatology, General Hospital of PLA, Beijing, China.
authors.forename: Bi-wen
authors.initials: BW
authors.lastname: Lin
authors.forename: Ze-long
authors.initials: ZL
authors.lastname: Jiao
authors.forename: Jian-feng
authors.initials: JF
authors.lastname: Fan
authors.forename: Liang
authors.initials: L
authors.lastname: Peng
authors.forename: Lei
authors.initials: L
authors.lastname: Li
authors.forename: Zi-gang
authors.initials: ZG
authors.lastname: Zhao
authors.forename: Xiang-yu
authors.initials: XY
authors.lastname: Ding
authors.forename: Heng-jin
authors.initials: HJ
authors.lastname: Li
mesh_terms: D016203:CDC2 Protein Kinase / Q000235:genetics / Q000378:metabolism; D045744:Cell Line, Tumor; D002465:Cell Movement; D015536:Down-Regulation; D059565:G2 Phase Cell Cycle Checkpoints; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000235:genetics / Q000378:metabolism; D007378:Interleukins / Q000235:genetics / Q000378:metabolism*; D059566:M Phase Cell Cycle Checkpoints; D020778:Matrix Metalloproteinase 2 / Q000235:genetics / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000235:genetics / Q000378:metabolism; D008545:Melanoma / Q000378:metabolism / Q000473:pathology; D016328:NF-kappa B / Q000235:genetics / Q000378:metabolism; D051059:PTEN Phosphohydrolase / Q000235:genetics / Q000378:metabolism; D010766:Phosphorylation; D051057:Proto-Oncogene Proteins c-akt / Q000235:genetics / Q000378:metabolism; D018808:Transcription Factor AP-1 / Q000235:genetics / Q000378:metabolism; D015854:Up-Regulation; D000099180:Interleukin-24
chemical_list: D016203:CDC2 Protein Kinase; D018799:Intercellular Adhesion Molecule-1; D007378:Interleukins; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9; D016328:NF-kappa B; D051059:PTEN Phosphohydrolase; D051057:Proto-Oncogene Proteins c-akt; D018808:Transcription Factor AP-1; D000099180:Interleukin-24; C494929:PTEN protein, human
keywords: Invasion; Melanoma; mda-7