title: Differential expression of miRNAs in esophageal cancer tissue.
abstract: The aim of this study was to explore the association of specific microRNAs (miRNAs) with the development of esophageal cancer (EC) and to identify new molecular markers for EC by analyzing the expression profiles of miRNAs in EC tissues. The expression profiles of miRNAs in paired EC and paracancerous normal tissues were detected and bioinformatically analyzed using miRNA assays. The outcomes were validated using real-time polymerase chain reaction. The miRNA assays revealed a total of 60 differentially expressed miRNAs in the EC tissues compared with those in the paracancerous normal tissues. Among them, 51 had doubled or more than doubled their expression levels and 9 had halved their expression levels. The most markedly upregulated miRNAs were hsa-miR-15a, hsa-miR-28-3p, hsa-miR-31, hsa-miR-99b, hsa-miR-101, hsa-miR-130a, hsa-miR-143, hsa-miR-196b, hsa-miR-200a, hsa-miR-210, hsa-miR-452 and hsa-miR-27a, whereas the most markedly downregulated miRNAs included hsa-miR-30b, hsa-miR-223, hsa-miR-454, hsa-miR-486, hsa-miR-574-3p and hsa-miR-126. Specific miRNA expression profiles exist in EC tissues and may serve as novel EC molecular markers.
authors.affiliation: Department of Thoracic Surgery, The First Affiliated Hospital of Xinxiang Medical University, Weihui 453100;
authors.forename: Shang-Guo
authors.initials: SG
authors.lastname: Liu
authors.forename: Xiu-Guang
authors.initials: XG
authors.lastname: Qin
authors.forename: Bao-Sheng
authors.initials: BS
authors.lastname: Zhao
authors.forename: Bo
authors.initials: B
authors.lastname: Qi
authors.forename: Wen-Jian
authors.initials: WJ
authors.lastname: Yao
authors.forename: Tian-Yun
authors.initials: TY
authors.lastname: Wang
authors.forename: Han-Chen
authors.initials: HC
authors.lastname: Li
authors.forename: Xiang-Nan
authors.initials: XN
authors.lastname: Wu
keywords: esophageal cancer; expression profile; gene chip; microRNA; real-time polymerase chain reaction