title: Effect of ionising radiation on polyphenolic content and antioxidant potential of parathion-treated sage (Salvia officinalis) leaves.
abstract: The γ-irradiation effects on polyphenolic content and antioxidant capacity of parathion-pretreated leaves of Salvia officinalis plant were investigated. The analysis of phenolic extracts of sage without parathion showed that irradiation decreased polyphenolic content significantly (p<0.05) by 30% and 45% at 2 and 4kGy, respectively, compared to non-irradiated samples. The same trend was observed for the Trolox equivalent antioxidant capacity (TEAC), as assessed by the anionic DPPH and cationic ABTS radical-scavenging assays. The antioxidant potential decreased significantly (p<0.01) at 2 and 4kGy, by 11-20% and 40-44%, respectively. The results obtained with a pure chlorogenic acid solution confirmed the degradation of phenols; however, its TEAC was significantly (p<0.01) increased following irradiation. Degradation products of parathion formed by irradiation seem to protect against a decline of antioxidant capacity and reduce polyphenolic loss. Ionising radiation was found to be useful in breaking down pesticide residues without inducing significant losses in polyphenols.
authors.affiliation: Laboratory of Protein Engineering and Bioactive Molecules (LIP-MB), National Institute of Applied Sciences and Technology (INSAT), University of Carthage, Tunis, Tunisia. issamcnstn@yahoo.fr
authors.forename: Issam
authors.initials: I
authors.lastname: Ben Salem
authors.forename: Sana
authors.initials: S
authors.lastname: Fekih
authors.forename: Haitham
authors.initials: H
authors.lastname: Sghaier
authors.forename: Mehrez
authors.initials: M
authors.lastname: Bousselmi
authors.forename: Mouldi
authors.initials: M
authors.lastname: Saidi
authors.forename: Ahmed
authors.initials: A
authors.lastname: Landoulsi
authors.forename: Sami
authors.initials: S
authors.lastname: Fattouch
mesh_terms: D000975:Antioxidants / Q000032:analysis*; D005514:Food Irradiation / Q000379:methods*; D005720:Gamma Rays; D007700:Kinetics; D015394:Molecular Structure; D010278:Parathion / Q000737:chemistry / Q000494:pharmacology*; D010573:Pesticide Residues / Q000737:chemistry / Q000494:pharmacology*; D010936:Plant Extracts / Q000032:analysis*; D018515:Plant Leaves / Q000737:chemistry / Q000187:drug effects / Q000528:radiation effects; D059808:Polyphenols / Q000032:analysis*; D027543:Salvia officinalis / Q000737:chemistry* / Q000187:drug effects / Q000528:radiation effects*
chemical_list: D000975:Antioxidants; D010573:Pesticide Residues; D010936:Plant Extracts; D059808:Polyphenols; D010278:Parathion