title: Cycloartenyl trans-ferulate, a component of the bran byproduct of sake-brewing rice, inhibits mammalian DNA polymerase and suppresses inflammation.
abstract: During the screening of selective DNA polymerase (pol) inhibitors, we isolated cycloartenyl trans-ferulate (CAF), which is a major component of γ-oryzanol, which is a byproduct formed during the production of Japanese rice wine "sake". CAF selectively inhibited the activity of mammalian A, B, and X pol families, but Y family pols were not affected. CAF did not influence the activities of plant or prokaryotic pols, nor the activity of other DNA metabolic enzymes tested. Individual chemical components of CAF, including cycloartenol (CA) and ferulic acid (FA), did not inhibit pol enzyme activities. CAF suppressed TPA (12-O-tetradecanoylphorbol-13-acetate)-induced inflammation in the mouse ear, but CA and FA did not. The ability to inhibit mammalian pol enzymes in vitro was positively correlated with their propensity to suppress inflammation in vivo. These results suggest that this byproduct formed during the sake-brewing process is useful as an anti-inflammatory agent.
authors.affiliation: Laboratory of Food & Nutritional Sciences, Faculty of Nutrition, Kobe Gakuin University, Nishi-ku, Kobe, Hyogo 651-2180, Japan. mizushin@nutr.kobegakuin.ac.jp
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Mizushina
authors.forename: Isoko
authors.initials: I
authors.lastname: Kuriyama
authors.forename: Asahi
authors.initials: A
authors.lastname: Yamazaki
authors.forename: Takahiro
authors.initials: T
authors.lastname: Akashi
authors.forename: Hiromi
authors.initials: H
authors.lastname: Yoshida
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D002417:Cattle; D003373:Coumaric Acids / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D004259:DNA-Directed DNA Polymerase / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D005260:Female; D006801:Humans; D007249:Inflammation / Q000188:drug therapy* / Q000276:immunology; D051379:Mice; D008813:Mice, Inbred ICR; D019384:Nucleic Acid Synthesis Inhibitors; D012275:Oryza / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D051381:Rats; D012639:Seeds / Q000737:chemistry; D014866:Waste Products / Q000032:analysis*; D014920:Wine / Q000032:analysis
chemical_list: D000893:Anti-Inflammatory Agents; D003373:Coumaric Acids; D004791:Enzyme Inhibitors; D019384:Nucleic Acid Synthesis Inhibitors; D010936:Plant Extracts; D014866:Waste Products; C540967:cycloartenyl ferulate; D004259:DNA-Directed DNA Polymerase