title: Microarray profiles of ex vivo expanded hematopoietic stem cells show induction of genes involved in noncanonical Wnt signaling.
abstract: The low number of hematopoietic stem cells (HSC) in umbilical cord blood (UCB) is directly related to increased risk of transplant failure. Effective ex vivo expansion of HSC has been tried for many years, with conflicting results because of the inability to reproduce in vitro HSC proliferation in the same way it occurs in vivo. We compared freshly isolated HSC with their expanded counterparts by microarray analysis and detected activation of the noncanonical Wnt (wingless-type MMTV integration site family) pathway. Study of early alterations during ex vivo UCB-HSC expansion could contribute to improvement of ex vivo expansion systems.
authors.affiliation: Departamento de Genética, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brasil. zanette@usp.br
authors.forename: D L
authors.initials: DL
authors.lastname: Zanette
authors.forename: J C C
authors.initials: JC
authors.lastname: Lorenzi
authors.forename: R A
authors.initials: RA
authors.lastname: Panepucci
authors.forename: A R D
authors.initials: AR
authors.lastname: Santos
authors.forename: G A
authors.initials: GA
authors.lastname: Molfetta
authors.forename: A G
authors.initials: AG
authors.lastname: Araujo
authors.forename: W A
authors.initials: WA
authors.lastname: Silva Junior
authors.forename: M A
authors.initials: MA
authors.lastname: Zago
mesh_terms: D051997:ADP-ribosyl Cyclase 1 / Q000378:metabolism; D018952:Antigens, CD34 / Q000378:metabolism; D002138:Calibration; D002452:Cell Count; D049109:Cell Proliferation; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000378:metabolism*; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis; D015203:Reproducibility of Results; D060449:Wnt Signaling Pathway / Q000235:genetics*
chemical_list: D018952:Antigens, CD34; D051997:ADP-ribosyl Cyclase 1