title: Combination of negative pressure wound therapy with open bone grafting for bone and soft tissue defects.
abstract: The aim of this study was to investigate the efficiency of negative pressure wound therapy (NPWT) combined with open bone graft (OBG; NPWT-OBG) for the treatment of bone and soft tissue defects with polluted wounds in an animal model. All rabbits with bone and soft tissue defects and polluted wounds were randomly divided into two groups, the experimental group (NPWT with bone graft) and the control group (OBG). The efficacy of the treatment was assessed by the wound conditions and healing time. Bacterial bioburdens and bony calluses were evaluated by bacteria counting and X-rays, respectively. Furthermore, granulation tissue samples from the wounds on days 0, 3, 7 and 14 of healing were evaluated for blood vessels and vascular endothelial growth factor (VEGF) levels. Wounds in the experimental group tended to have a shorter healing time, healthier wound conditions, lower bacterial bioburden, improvement of the bony calluses and an increased blood supply compared with those in the control group. With NPWT, wound infection was effectively controlled. For wounds with osseous and soft tissue defects, NPWT combined with bone grafting was demonstrated to be more effective than an OBG.
authors.affiliation: Department of Micro-Orthopedics, Zhongnan Hospital of Wuhan University, Wuhan, Hubei 430071, P.R. China.
authors.forename: Kai
authors.initials: K
authors.lastname: Deng
authors.forename: Ai-Xi
authors.initials: AX
authors.lastname: Yu
authors.forename: Cheng-Yan
authors.initials: CY
authors.lastname: Xia
authors.forename: Zong-Huan
authors.initials: ZH
authors.lastname: Li
authors.forename: Wei-Yang
authors.initials: WY
authors.lastname: Wang
mesh_terms: D000818:Animals; D058491:Bacterial Load; D016025:Bone Transplantation; D001842:Bone and Bones / Q000293:injuries* / Q000382:microbiology; D004195:Disease Models, Animal; D054843:Negative-Pressure Wound Therapy; D018919:Neovascularization, Physiologic; D011817:Rabbits; D017695:Soft Tissue Injuries / Q000382:microbiology / Q000628:therapy*; D016896:Treatment Outcome; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism; D014945:Wound Healing; D014947:Wounds and Injuries / Q000175:diagnosis / Q000209:etiology* / Q000628:therapy*
chemical_list: D042461:Vascular Endothelial Growth Factor A