title: Ectoine production from lignocellulosic biomass-derived sugars by engineered Halomonas elongata.
abstract: In this study, the water-retaining cyclic amino acid ectoine was produced from a variety of sugars, including glucose, xylose, cellobiose, and glucose/xylose mixture using engineered Halomonas elongata. When grown on xylose as the sole carbon source, H. elongata produced 333 mmol/kg fresh cell weight (FW) of ectoine, which was 1.4-fold higher than that produced from glucose. To improve ectoine production, an ectD deficient H. elongata mutant was constructed. The engineered H. elongata produced 377 mmol/kg FW of ectoine from a glucose/xylose mixture. Ectoine was also produced from rice straw hydrolysate. These results show that H. elongata can produce ectoine from a variety of sugars derived from lignocellulosic biomass and thus has tremendous potential as a host for producing useful compounds from biomass resources.
authors.affiliation: Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1, Rokkodai-Cho, Nada, Kobe 657-8501, Japan.
authors.forename: Kosuke
authors.initials: K
authors.lastname: Tanimura
authors.forename: Hideki
authors.initials: H
authors.lastname: Nakayama
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Tanaka
authors.forename: Akihiko
authors.initials: A
authors.lastname: Kondo
mesh_terms: D000599:Amino Acids, Diamino / Q000096:biosynthesis*; D018533:Biomass; D005818:Genetic Engineering; D020632:Halomonas / Q000235:genetics / Q000378:metabolism*; D008031:Lignin / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D000599:Amino Acids, Diamino; C036909:lignocellulose; C045628:ectoine; D008031:Lignin
keywords: Ectoine; Halomonas elongata; Lignocellulosic biomass; Rice straw hydrolysate; Xylose