title: Extraction, antioxidant capacity and identification of Semen Astragali Complanati (Astragalus complanatus R. Br.) phenolics.
abstract: A study about the ultrasonic extraction, antioxidant capacity and identification of phenolics from Semen Astragali Complanati was undertaken. The optimised extraction condition with an orthogonal experiment design of L9(3(4)) was 50°C, 30min and 15:1 (methanol solution:sample, mL:g). An extraction yield of 40.12±1.10mg gallic acid/g dry seed and the antioxidant capacity of 0.85±0.13mg dry extract/mg DPPH were obtained with the optimum condition, respectively. For each extraction set, the antioxidant capacity by 2,2-diphenyl-1-picrylhydrazyl radical assay highly correlated to its total phenolic content by Folin-Ciocalteu method. Twelve phenolic components obtained with the optimised extraction procedure were tentatively identified by Ultra Performance Liquid Chromatography coupled with Q-TOF Mass Spectrometer (UPLC-Q-TOF) according to their mass spectrometry, UV/vis spectrometry, and related literature reports. Furthermore, the ultrasonic effect on the particles' microstructure of Semen Astragali Complanati was also investigated by scanning electron microscopy (SEM) analysis.
authors.affiliation: School of Food Engineering and Nutrition Science, Shaanxi Normal University, Xi'an 710062, PR China. qinganzhang@snnu.edu.cn
authors.forename: Qing-An
authors.initials: QA
authors.lastname: Zhang
authors.forename: Xue-Hui
authors.initials: XH
authors.lastname: Fan
authors.forename: Zhi-Qi
authors.initials: ZQ
authors.lastname: Zhang
authors.forename: Tao
authors.initials: T
authors.lastname: Li
authors.forename: Cai-Ping
authors.initials: CP
authors.lastname: Zhu
authors.forename: Xiao-Rui
authors.initials: XR
authors.lastname: Zhang
authors.forename: Wei
authors.initials: W
authors.lastname: Song
mesh_terms: D000975:Antioxidants / Q000737:chemistry* / Q000302:isolation & purification*; D027883:Astragalus Plant / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D013058:Mass Spectrometry; D010636:Phenols / Q000737:chemistry* / Q000302:isolation & purification*; D010936:Plant Extracts / Q000737:chemistry* / Q000302:isolation & purification*
chemical_list: D000975:Antioxidants; D010636:Phenols; D010936:Plant Extracts