title: Glutathione and ascorbic acid protect Arabidopsis plants against detrimental effects of iron deficiency.
abstract: Iron is an essential micronutrient required for a wide variety of cellular functions in plant growth and development. Chlorosis is the first visible symptom in iron-deficient plants. Glutathione (GSH) and ascorbic acid (ASC) are multifunctional metabolites playing important roles in redox balancing. In this work, it was shown that GSH and ASC treatment prevented chlorosis and the accumulation of reactive oxygen species induced by iron deficiency in Arabidopsis leaves. In iron deficiency, GSH and ASC increased the activity of the heme protein ascorbate peroxidase at a similar level to that found in iron-sufficient seedlings. GSH was also able to preserve the levels of the iron-sulfur protein ferredoxin 2. GSH content decreased 25% in iron-deficient Arabidopsis seedlings, whereas the ASC levels were not affected. Taken together, these results showed that GSH and ASC supplementation protects Arabidopsis seedlings from iron deficiency, preserving cell redox homeostasis and improving internal iron availability.
authors.affiliation: Instituto de Investigaciones Biológicas, UE-CONICET-UNMdP, Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Mar del Plata, Funes 3250, CC 1245, 7600, Mar del Plata, Argentina.
authors.forename: Leonor
authors.initials: L
authors.lastname: Ramírez
authors.forename: Carlos Guillermo
authors.initials: CG
authors.lastname: Bartoli
authors.forename: Lorenzo
authors.initials: L
authors.lastname: Lamattina
mesh_terms: D017360:Arabidopsis / Q000187:drug effects / Q000378:metabolism*; D001205:Ascorbic Acid / Q000494:pharmacology*; D005978:Glutathione / Q000494:pharmacology*; D000090463:Iron Deficiencies; D010935:Plant Diseases / Q000517:prevention & control; D026361:Reactive Nitrogen Species / Q000378:metabolism
chemical_list: D026361:Reactive Nitrogen Species; D005978:Glutathione; D001205:Ascorbic Acid
keywords: Arabidopsis thaliana; ascorbic acid; chlorosis; glutathione; iron deficiency; reactive oxygen species.