title: Chemically modified siRNAs and miRNAs bearing urea/thiourea-bridged aromatic compounds at their 3'-end for RNAi therapy.
abstract: We have developed chemically modified siRNAs and miRNAs bearing urea/thiourea-bridged aromatic compounds at their 3'-end for RNAi therapy. Chemically modified RNAs possessing urea/thiourea-bridged aromatic compounds instead of naturally occurring dinucleotides at the 3'-overhang region were easily prepared in good yields and were more resistant to nucleolytic hydrolysis than unmodified RNA. siRNAs containing urea or thiourea derivatives showed the desired knockdown effect. Furthermore, modified miR-143 duplexes carrying the urea/thiourea compounds in the 3'-end of each strand were able to inhibit the growth of human bladder cancer T24 cells.
authors.affiliation: Department of Biomolecular Science, Graduate School of Engineering, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan.
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Kitamura
authors.forename: Yuki
authors.initials: Y
authors.lastname: Masegi
authors.forename: Shunsuke
authors.initials: S
authors.lastname: Ogawa
authors.forename: Remi
authors.initials: R
authors.lastname: Nakashima
authors.forename: Yukihiro
authors.initials: Y
authors.lastname: Akao
authors.forename: Yoshihito
authors.initials: Y
authors.lastname: Ueno
authors.forename: Yukio
authors.initials: Y
authors.lastname: Kitade
mesh_terms: D001952:Bridged-Ring Compounds; D045744:Cell Line, Tumor; D015316:Genetic Therapy / Q000379:methods*; D006367:HeLa Cells; D006801:Humans; D035683:MicroRNAs / Q000737:chemistry* / Q000235:genetics / Q000494:pharmacology; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000737:chemistry* / Q000235:genetics / Q000494:pharmacology; D013890:Thiourea / Q000737:chemistry*; D014162:Transfection; D014508:Urea / Q000737:chemistry*
chemical_list: D001952:Bridged-Ring Compounds; D035683:MicroRNAs; D034741:RNA, Small Interfering; D014508:Urea; D013890:Thiourea