title: Low RIN1 expression in HCC is associated with tumor invasion and unfavorable prognosis.
abstract: OBJECTIVE
  To explore the association between the expression of Ras and Rab interactor 1 (RIN1) and the prognosis of hepatocellular carcinoma (HCC).
  
  
  METHODS
  RIN1 expression was detected in paired HCC tissues by real-time polymerase chain reaction, Western blot analysis, and immunohistochemistry. Transfection was applied to analyze the RIN1 function.
  
  
  RESULTS
  We found that expression of the RIN1 protein was downregulated in the HCC samples compared with the corresponding normal tissues. Downregulation of RIN1 expression was also associated with invasion and poor overall survival (OS). The results of our multivariate analysis indicated that the RIN1 status is a significant prognostic factor for OS. RIN1 overexpression also inhibited cell invasion in HepG2 cells. The expression between RIN1 and ABL2 may present a positive correlation.
  
  
  CONCLUSIONS
  Our results demonstrate that RIN1 suppresses tumor invasion in HCC patients and that a poor prognosis for HCC is expected when RIN1 expression is downregulated.
authors.affiliation: Dept. of General Surgery, the First Affiliated Hospital of China Medical University, Shenyang, 110001 Liaoning, China.
authors.forename: Hui
authors.initials: H
authors.lastname: He
authors.forename: Gang
authors.initials: G
authors.lastname: Wu
authors.forename: Haiyang
authors.initials: H
authors.lastname: Liu
authors.forename: Ying
authors.initials: Y
authors.lastname: Cheng
authors.forename: Yanqiu
authors.initials: Y
authors.lastname: Yu
authors.forename: Yawei
authors.initials: Y
authors.lastname: Wang
authors.forename: Yongfeng
authors.initials: Y
authors.lastname: Liu
mesh_terms: D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism*; D006528:Carcinoma, Hepatocellular / Q000378:metabolism / Q000473:pathology*; D002681:China; D015536:Down-Regulation; D005260:Female; D005500:Follow-Up Studies; D015870:Gene Expression; D015972:Gene Expression Regulation, Neoplastic; D016147:Genes, Tumor Suppressor; D056945:Hep G2 Cells; D006801:Humans; D007150:Immunohistochemistry; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism*; D008113:Liver Neoplasms / Q000378:metabolism / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D015999:Multivariate Analysis; D009361:Neoplasm Invasiveness; D011379:Prognosis; D012334:RNA, Neoplasm / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D014408:Biomarkers, Tumor; D047908:Intracellular Signaling Peptides and Proteins; C092117:RIN1 protein, human; D012334:RNA, Neoplasm
keywords: ABL2; HCC; Hepatocellular carcinoma; RIN1; Ras and Rab interactor 1