title: Engineering of epidermis skin grafts using electrospun nanofibrous gelatin/ polycaprolactone membranes.
abstract: Skin engineering provides a new strategy for treating a wide variety of skin defects. In particular, electrospun nanofibrous membranes have been used as carriers for epidermis engineering. The aim of this study was to investigate the feasibility of a modified gelatin and polycaprolactone (GT/PCL) electrospun membrane for epidermis engineering. The biocompatibility of the membranes was evaluated by seeding HaCaT cells (human keratinocyte cell line) on the membrane and the mechanical properties of the membranes were determined with and without cells after culture. A cell proliferation assay showing that HaCaT cells attached and proliferated well on the membranes demonstrated that the membranes possess good biocompatibility. Mechanical tests showed that the membranes are strong enough to be handled during transplantation. Further in vivo transplantation studies revealed that epidermises engineered with GT/PCL membranes were able to repair skin defects in the nude mouse. These results demonstrate that GT/PCL electrospun membranes could be suitable scaffolds for skin engineering.
authors.affiliation: Department of Plastic and Reconstructive Surgery, Shanghai 9th People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Tissue Engineering, National Tissue Engineering Center of China, Shanghai, People's Republic of China.
authors.forename: Huichuan
authors.initials: H
authors.lastname: Duan
authors.forename: Bei
authors.initials: B
authors.lastname: Feng
authors.forename: Xiangkai
authors.initials: X
authors.lastname: Guo
authors.forename: Jiaming
authors.initials: J
authors.lastname: Wang
authors.forename: Li
authors.initials: L
authors.lastname: Zhao
authors.forename: Guangdong
authors.initials: G
authors.lastname: Zhou
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.forename: Yilin
authors.initials: Y
authors.lastname: Cao
authors.forename: Wen Jie
authors.initials: WJ
authors.lastname: Zhang
mesh_terms: D000818:Animals; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D004817:Epidermis / Q000187:drug effects / Q000293:injuries; D005780:Gelatin / Q000737:chemistry* / Q000494:pharmacology; D006801:Humans; D008297:Male; D008567:Membranes, Artificial; D051379:Mice; D008819:Mice, Nude; D057139:Nanofibers / Q000737:chemistry*; D036103:Nanotechnology / Q000379:methods; D011091:Polyesters / Q000737:chemistry* / Q000494:pharmacology; D054457:Tissue Scaffolds / Q000737:chemistry*; D014945:Wound Healing / Q000187:drug effects
chemical_list: D008567:Membranes, Artificial; D011091:Polyesters; C016240:polycaprolactone; D005780:Gelatin
keywords: electrospun nanofibrous membrane; epidermis engineering; gelatin; polycaprolactone