title: RP-UPLC method development and validation for the simultaneous estimation of ibuprofen and famotidine in pharmaceutical dosage form.
abstract: OBJECTIVE
  A stability-indicating LC method was developed for the simultaneous determination of Ibuprofen and Famotidine in pharmaceutical dosage forms.
  
  
  METHODS
  The chromatographic separation was achieved on Acquity UPLC BEH C-18,50 mm × 2.1 mm and 1.7 μm column with gradient elution. The mobile phase A contains a mixture of 50 mM sodium acetate buffer (pH 5.5): methanol (85:15, v/v), and the mobile phase B contains a mixture of 50 mM sodium acetate buffer (pH 5.5): methanol (25:75, v/v). The flow rate was 0.3 mL min(-1), and the detection wavelength was 260 nm.
  
  
  RESULTS
  The limit of detection for Ibuprofen and Famotidine was 1.6 and 1.2 μg mL(-1), respectively. The limit of quantification (LOQ) for Ibuprofen and Famotidine was 5.1 and 4.3 μg mL(-1), respectively.
  
  
  CONCLUSIONS
  This method was validated for accuracy, precision, and linearity. The method was also found to be stability indicating.
authors.affiliation: Department of Chemical Science and Technology, Institute of science and Technology, Jawaharlal Nehru Technological University, Hyderabad, India.
authors.forename: Yarram Ramakoti
authors.initials: YR
authors.lastname: Reddy
authors.forename: Kakumani Kishore
authors.initials: KK
authors.lastname: Kumar
authors.forename: Mrp
authors.initials: M
authors.lastname: Reddy
authors.forename: K
authors.initials: K
authors.lastname: Mukkanti
keywords: Famotidine; UPLC; ibuprofen; ssimultaneous; sstability-indicating