title: Reactive astrocytes associated with plaques in TgCRND8 mouse brain and in human Alzheimer brain express phosphoprotein enriched in astrocytes (PEA-15).
abstract: To identify potential biomarkers associated with Alzheimer's disease (AD)-like neuropathologies in the murine brain, we conducted proteomic analyses of neocortices from TgCRND8 mice. Here we found that phosphoprotein enriched in astrocytes 15 kDa (PEA-15) is expressed at higher levels in the neocortical proteomes from 6-month old TgCRND8 mice, as compared to non-transgenic mice. Immunostaining for PEA-15 revealed reactive astrocytes associated with the neocortical amyloid plaques in TgCRND8 mice and in post-mortem human AD brains. This is the first report of increased phosphoprotein enriched in astrocytes (PEA-15) expression in reactive astrocytes of an AD mouse model and human AD brains.
authors.affiliation: Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, Ontario, Canada.
authors.forename: Lynsie A M
authors.initials: LA
authors.lastname: Thomason
authors.forename: Laura J
authors.initials: LJ
authors.lastname: Smithson
authors.forename: Lili-Naz
authors.initials: LN
authors.lastname: Hazrati
authors.forename: JoAnne
authors.initials: J
authors.lastname: McLaurin
authors.forename: Michael D
authors.initials: MD
authors.lastname: Kawaja
mesh_terms: D000544:Alzheimer Disease / Q000378:metabolism* / Q000473:pathology; D000818:Animals; D001253:Astrocytes / Q000378:metabolism* / Q000502:physiology*; D001921:Brain / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D051379:Mice; D008822:Mice, Transgenic; D010750:Phosphoproteins / Q000378:metabolism*
chemical_list: D010750:Phosphoproteins
keywords: AD; Alzheimer disease; Alzheimer’s; Astrocyte; DED; Human; PEA-15; TgCRND8; death effector domain; phosphoprotein enriched in astrocytes