title: Inorganic phosphate export by the retrovirus receptor XPR1 in metazoans.
abstract: Inorganic phosphate uptake is a universal function accomplished by transporters that are present across the living world. In contrast, no phosphate exporter has ever been identified in metazoans. Here, we show that depletion of XPR1, a multipass membrane molecule initially identified as the cell-surface receptor for xenotropic and polytropic murine leukemia retroviruses (X- and P-MLV), induced a decrease in phosphate export and that reintroduction of various XPR1 proteins, from fruit fly to human, rescued this defect. Inhibition of phosphate export was also obtained with a soluble ligand generated from the envelope-receptor-binding domain of X-MLV in all human cell lines tested, as well as in diverse stem cells and epithelial cells derived from renal proximal tubules, the main site of phosphate homeostasis regulation. These results provide new insights on phosphate export in metazoans and the role of Xpr1 in this function.
authors.affiliation: Institut de Génétique Moléculaire de Montpellier UMR 5535 CNRS, 1919 route de Mende, 34293 Montpellier cedex 5, France.
authors.forename: Donatella
authors.initials: D
authors.lastname: Giovannini
authors.forename: Jawida
authors.initials: J
authors.lastname: Touhami
authors.forename: Pierre
authors.initials: P
authors.lastname: Charnet
authors.forename: Marc
authors.initials: M
authors.lastname: Sitbon
authors.forename: Jean-Luc
authors.initials: JL
authors.lastname: Battini
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D016466:CHO Cells; D018938:Caco-2 Cells; D003412:Cricetulus; D057809:HEK293 Cells; D006367:HeLa Cells; D006801:Humans; D019169:Jurkat Cells; D051379:Mice; D008954:Models, Biological; D008969:Molecular Sequence Data; D041681:NIH 3T3 Cells; D010710:Phosphates / Q000378:metabolism*; D043562:Receptors, G-Protein-Coupled / Q000235:genetics / Q000378:metabolism*; D011991:Receptors, Virus / Q000235:genetics / Q000378:metabolism*; D013045:Species Specificity; D014162:Transfection; D000089842:Xenotropic and Polytropic Retrovirus Receptor
chemical_list: D010710:Phosphates; D043562:Receptors, G-Protein-Coupled; D011991:Receptors, Virus; C000715267:XPR1 protein, human; D000089842:Xenotropic and Polytropic Retrovirus Receptor; C000715268:Xpr1 protein, mouse