title: A bio-nanocapsule containing envelope protein domain III of Japanese encephalitis virus protects mice against lethal Japanese encephalitis virus infection.
abstract: An engineered bio-nanocapsule (BNC) comprising modified hepatitis B surface antigen L protein was used as a physical scaffold for envelope protein domain III (D3) of Japanese encephalitis virus (JEV). At the N terminus, the BNC contained a two-tandem repeat of the Z domain (ZZ) derived from Staphylococcus aureus protein A (ZZ-BNC). The Lys-rich ZZ moiety exposed on the surface of ZZ-BNC was used for chemical conjugation with the JEV D3 antigen, which had been expressed and purified from Escherichia coli. Immunization of mice with D3 loaded on the surface of ZZ-BNC (ZZ-BNC:D3) augmented serum IgG response against JEV and increased protection against lethal JEV infection. The present study suggests that innocuous recombinant antigens, when loaded on the surface of ZZ-BNC, can be transformed to immunogenic antigens.
authors.affiliation: Molecular Microbiology Group, Department of Tropical Infectious Diseases, COMB, Tropical Biosphere Research Center, Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Miyata
authors.forename: Senji
authors.initials: S
authors.lastname: Tafuku
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Harakuni
authors.forename: Masayuki
authors.initials: M
authors.lastname: Tadano
authors.forename: Nobuo
authors.initials: N
authors.lastname: Yoshimoto
authors.forename: Masumi
authors.initials: M
authors.lastname: Iijima
authors.forename: Hidenori
authors.initials: H
authors.lastname: Matsuo
authors.forename: Goro
authors.initials: G
authors.lastname: Matsuzaki
authors.forename: Shun'ichi
authors.initials: S
authors.lastname: Kuroda
authors.forename: Takeshi
authors.initials: T
authors.lastname: Arakawa
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D004337:Drug Carriers / Q000008:administration & dosage*; D004664:Encephalitis Virus, Japanese / Q000235:genetics / Q000276:immunology*; D004926:Escherichia coli / Q000235:genetics; D005260:Female; D007074:Immunoglobulin G / Q000097:blood; D022321:Japanese Encephalitis Vaccines / Q000008:administration & dosage / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D053769:Nanocapsules / Q000008:administration & dosage*; D011485:Protein Binding; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology; D013205:Staphylococcal Protein A / Q000235:genetics / Q000378:metabolism; D016019:Survival Analysis; D014611:Vaccination / Q000379:methods; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000276:immunology; D014759:Viral Envelope Proteins / Q000235:genetics / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D004337:Drug Carriers; D007074:Immunoglobulin G; D022321:Japanese Encephalitis Vaccines; D053769:Nanocapsules; D011994:Recombinant Proteins; D013205:Staphylococcal Protein A; D014614:Vaccines, Synthetic; D014759:Viral Envelope Proteins