title: siRNA-chitosan complexes in poly(lactic-co-glycolic acid) nanoparticles for the silencing of aquaporin-1 in cancer cells.
abstract: A large number of studies document the strong expression of aquaporin-1 (AQP1) in tumor microvessels and correlate this aberrant expression with higher metastatic potential and aggressiveness of the malignancy. Although small animal experiments have shown that the modulation of AQP1 expression can halt angiogenesis and induce tumor regression, effective and safe strategies for the tissue specific inhibition of AQP1 are still missing. Here, small interference RNA-chitosan complexes encapsulated in poly(lactic-co-glycolic acid) (PLGA) nanoparticles (NPs) are proposed for the intracellular delivery of siRNA molecules against AQP1. These NPs are coated with poly(vinyl alcohol) (PVA), to improve stability under physiological conditions, and demonstrate a diameter of 160 nm. The partial neutralization of the negatively charged siRNA molecules with the cationic chitosan enhances the loading by 5-fold, as compared to that of the free siRNA molecules, and allows one to modulate the release kinetics in the pH-dependent manner. At pH = 7.4, mimicking the conditions found in the systemic circulation, only the 40% of siRNA is released at 24 h post incubation; whereas at pH = 5.0, recreating the cell endosomal environment, all siRNA molecules are released in about 3 h. These NPs show no cytotoxicity on HeLa cells up to 72 h of incubation. In the same cells, transfected to overexpress AQP1, a silencing efficiency of 70% is achieved at 24 h post treatment with siRNA-loaded NPs. Confocal microscopy analysis of NP uptake demonstrates that siRNA molecules accumulate perinuclearly and in the nucleus. Given the stability, preferential release behavior, and well-known biocompatibility properties of PLGA nanostructures, these siRNA-loaded NPs hold potential for the efficient and safe in vivo silencing of AQPs via systemic administration.
authors.affiliation: Department of Translational Imaging and Department of Nanomedicine, The Methodist Hospital Research Institute, Houston, Texas 77030, United States.
authors.forename: Cinzia
authors.initials: C
authors.lastname: Stigliano
authors.forename: Santosh
authors.initials: S
authors.lastname: Aryal
authors.forename: Marco Donato
authors.initials: MD
authors.lastname: de Tullio
authors.forename: Grazia Paola
authors.initials: GP
authors.lastname: Nicchia
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Pascazio
authors.forename: Maria
authors.initials: M
authors.lastname: Svelto
authors.forename: Paolo
authors.initials: P
authors.lastname: Decuzzi
mesh_terms: D051398:Aquaporin 1 / Q000235:genetics*; D048271:Chitosan / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D019344:Lactic Acid / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry*; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011108:Polymers / Q000737:chemistry; D034741:RNA, Small Interfering / Q000235:genetics*
chemical_list: D011108:Polymers; D034741:RNA, Small Interfering; D051398:Aquaporin 1; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D048271:Chitosan