title: A mechanochemical model of cell reorientation on substrates under cyclic stretch.
abstract: We report a theoretical study on the cyclic stretch-induced reorientation of spindle-shaped cells. Specifically, by taking into account the evolution of sub-cellular structures like the contractile stress fibers and adhesive receptor-ligand clusters, we develop a mechanochemical model to describe the dynamics of cell realignment in response to cyclically stretched substrates. Our main hypothesis is that cells tend to orient in the direction where the formation of stress fibers is energetically most favorable. We show that, when subjected to cyclic stretch, the final alignment of cells reflects the competition between the elevated force within stress fibers that accelerates their disassembly and the disruption of cell-substrate adhesion as well, and an effectively increased substrate rigidity that promotes more stable focal adhesions. Our model predictions are consistent with various observations like the substrate rigidity dependent formation of stable adhesions and the stretching frequency, as well as stretching amplitude, dependence of cell realignment. This theory also provides a simple explanation on the regulation of protein Rho in the formation of stretch-induced stress fibers in cells.
authors.affiliation: Department of Engineering Mechanics, Soft Matter Research Center, Zhejiang University, Hangzhou, Zhejiang, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Qian
authors.forename: Haipei
authors.initials: H
authors.lastname: Liu
authors.forename: Yuan
authors.initials: Y
authors.lastname: Lin
authors.forename: Weiqiu
authors.initials: W
authors.lastname: Chen
authors.forename: Huajian
authors.initials: H
authors.lastname: Gao
mesh_terms: D000818:Animals; D001696:Biomechanical Phenomena; D002448:Cell Adhesion; D048430:Cell Shape; D002478:Cells, Cultured; D003599:Cytoskeleton / Q000737:chemistry / Q000502:physiology*; D005057:Eukaryotic Cells / Q000166:cytology* / Q000378:metabolism / Q000502:physiology; D022001:Focal Adhesions / Q000737:chemistry / Q000502:physiology*; D006801:Humans; D008954:Models, Biological; D022502:Stress Fibers / Q000737:chemistry / Q000502:physiology*; D013314:Stress, Mechanical; D013816:Thermodynamics; D020741:rho GTP-Binding Proteins / Q000502:physiology*
chemical_list: D020741:rho GTP-Binding Proteins