title: Efficient production of bioethanol from corn stover by pretreatment with a combination of sulfuric acid and sodium hydroxide.
abstract: Corn stover is the most abundant agricultural residue in China and a valuable reservoir for bioethanol production. In this study, we proposed a process for producing bioethanol from corn stover; the pretreatment prior to presaccharification, followed by simultaneous saccharification and fermentation (SSF) by using a flocculating Saccharomyces cerevisiae strain, was optimized. Pretreatment with acid-alkali combination (1% H2SO4, 150 °C, 10 min, followed by 1% NaOH, 80°C, 60 min) resulted in efficient lignin removal and excellent recovery of xylose and glucose. A glucose recovery efficiency of 92.3% was obtained by enzymatic saccharification, when the pretreated solid load was 15%. SSF was carried out at 35 °C for 36 hr after presaccharification at 50 °C for 24 hr, and an ethanol yield of 88.2% was achieved at a solid load of 15% and an enzyme dosage of 15 FPU/g pretreated corn stover.
authors.affiliation: Graduate School of Science and Technology, Kumamoto University, Kumamoto City, Kumamoto, Japan.
authors.forename: Li
authors.initials: L
authors.lastname: Tan
authors.forename: Yue-Qin
authors.initials: YQ
authors.lastname: Tang
authors.forename: Hiroto
authors.initials: H
authors.lastname: Nishimura
authors.forename: Shouta
authors.initials: S
authors.lastname: Takei
authors.forename: Shigeru
authors.initials: S
authors.lastname: Morimura
authors.forename: Kenji
authors.initials: K
authors.lastname: Kida
mesh_terms: D001709:Biotechnology / Q000379:methods; D000431:Ethanol / Q000138:chemical synthesis*; D005285:Fermentation; D005947:Glucose / Q000737:chemistry / Q000378:metabolism; D006868:Hydrolysis; D008031:Lignin / Q000737:chemistry; D012441:Saccharomyces cerevisiae / Q000737:chemistry / Q000378:metabolism; D012972:Sodium Hydroxide / Q000737:chemistry*; D013464:Sulfuric Acids / Q000737:chemistry*; D003313:Zea mays / Q000737:chemistry*
chemical_list: D013464:Sulfuric Acids; D000431:Ethanol; D012972:Sodium Hydroxide; D008031:Lignin; D005947:Glucose; C033158:sulfuric acid