title: Mutagenic effect of Bisphenol A on adult rat male germ cells and their fertility.
abstract: The present study investigates the effects of Bisphenol A on the induction of dominant lethal mutation and male reproductive functions. The male rats were gavaged with BPA (10 μg, and 5.0 mg/kg/bw) over a period of six days and control group with vehicle. Each male was cohabited with untreated females sequentially over the period of eight weeks. The mated females were sacrificed on 15th day of gestation. The results revealed a significant increase in dominant lethal mutation rate during fourth and sixth week of mating intervals at 5.0mg/kgbw dose of BPA. These findings demonstrate that mid-spermatids and spermatocytes are more sensitive to BPA exposure. The male rats sacrificed at the end of mating study showed an increase in the sperm DNA damage, and decrease in motility at higher dose. However, significant reductions in sperm production effects were observed at both lower and higher doses of BPA. These preliminary results indicate that BPA may be a weak male germ cell mutagen.
authors.affiliation: National Center for Preclinical Reproductive and Genetic Toxicology, National Institute for Research in Reproductive Health, Parel, Mumbai, India.
authors.forename: Dinesh
authors.initials: D
authors.lastname: Tiwari
authors.forename: Geeta
authors.initials: G
authors.lastname: Vanage
mesh_terms: D000818:Animals; D001559:Benzhydryl Compounds / Q000633:toxicity*; D020552:Comet Assay; D052244:Endocrine Disruptors / Q000633:toxicity*; D004968:Estrogens, Non-Steroidal / Q000633:toxicity*; D005260:Female; D005298:Fertility / Q000187:drug effects; D008297:Male; D009153:Mutagens / Q000633:toxicity*; D010636:Phenols / Q000633:toxicity*; D011247:Pregnancy; D051381:Rats; D017207:Rats, Sprague-Dawley; D013076:Sperm Count; D013081:Sperm Motility; D013091:Spermatogenesis / Q000187:drug effects; D013094:Spermatozoa / Q000187:drug effects* / Q000502:physiology; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D052244:Endocrine Disruptors; D004968:Estrogens, Non-Steroidal; D009153:Mutagens; D010636:Phenols; C006780:bisphenol A; D000098826:Bisphenol A Compounds
keywords: Bisphenol A; Daily sperm production; Dominant lethal mutation; Post-implantation loss; Sperm count; Spermatogenesis germ cells