title: [Influence of hairpin RNA, specific to the gene lawc, on expression of overlapping genes of the lawc/Trf2 complex in D. melanogaster].
abstract: We used a genetic system of the gene complex leg-arista-wing complex/TBP related factor 2 (lawc/Trf2) as a suitable model for in vivo study of operational characteristics of divergent overlapping genes. We established lines of transgenic fruit flies carrying constructs capable of expressing hairpin RNA directed at post-transcriptional suppression of the mRNA gene lawc by way of RNA-interference. The attempt to artificially suppress lawc-transcripts caused a rise in the level of expression of the gene lawc by several times. It is shown that change in the concentration of lawc-transcripts can affect the concentration of Trf2 transcripts. Possible mechanisms of regulation of expression of the overlapping genes studied are discussed.
authors.forename: R O
authors.initials: RO
authors.lastname: Cherezov
authors.forename: Iu E
authors.initials: IuE
authors.lastname: Vorontsova
authors.forename: I B
authors.initials: IB
authors.lastname: Mertsalov
authors.forename: D A
authors.initials: DA
authors.lastname: Kulikova
authors.forename: O B
authors.initials: OB
authors.lastname: Simonova
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D029721:Drosophila Proteins / Q000235:genetics* / Q000378:metabolism; D004331:Drosophila melanogaster / Q000196:embryology / Q000235:genetics*; D018507:Gene Expression Regulation, Developmental; D005807:Genes, Overlapping; D034622:RNA Interference; D034741:RNA, Small Interfering; D035341:Telomeric Repeat Binding Protein 2 / Q000235:genetics* / Q000378:metabolism
chemical_list: D029721:Drosophila Proteins; D034741:RNA, Small Interfering; C509085:TRF2 protein, Drosophila; D035341:Telomeric Repeat Binding Protein 2