title: The microglial activation state regulates migration and roles of matrix-dissolving enzymes for invasion.
abstract: BACKGROUND
  Microglial cells are highly mobile under many circumstances and, after central nervous system (CNS) damage, they must contend with the dense extracellular matrix (ECM) in order to reach their target sites. In response to damage or disease, microglia undergo complex activation processes that can be modulated by environmental cues and culminate in either detrimental or beneficial outcomes. Thus, there is considerable interest in comparing their pro-inflammatory ('classical' activation) and resolving 'alternative' activation states. Almost nothing is known about how these activation states affect the ability of microglia to migrate and degrade ECM, or the enzymes used for substrate degradation. This is the subject of the present study.
  
  
  METHODS
  Primary cultured rat microglial cells were exposed to lipopolysaccharide (LPS) to evoke classical activation or IL4 to evoke alternative activation. High-resolution microscopy was used to monitor changes in cell morphology and aspects of the cytoskeleton. We quantified migration in a scratch-wound assay and through open filter holes, and invasion through Matrigel™. A panel of inhibitors was used to analyze contributions of different matrix-degrading enzymes to migration and invasion, and quantitative real-time reverse transcriptase PCR (qRT-PCR) was used to assess changes in their expression.
  
  
  RESULTS
  Vinculin- and F-actin-rich lamellae were prominent in untreated and IL4-treated microglia (but not after LPS). IL4 increased the migratory capacity of microglia but eliminated the preferential anterior nuclear-centrosomal axis polarity and location of the microtubule organizing center (MTOC). Microglia degraded fibronectin, regardless of treatment, but LPS-treated cells were relatively immobile and IL4-treated cells invaded much more effectively through Matrigel™. For invasion, untreated microglia primarily used cysteine proteases, but IL4-treated cells used a wider range of enzymes (cysteine proteases, cathepsin S and K, heparanase, and matrix metalloproteases). Untreated microglia expressed MMP2, MMP12, heparanase, and four cathepsins (B, K, L1, and S). Each activation stimulus upregulated a different subset of enzymes. IL4 increased MMP2 and cathepsins S and K; whereas LPS increased MMP9, MMP12, MMP14 (MT1-MMP), heparanase, and cathepsin L1.
  
  
  CONCLUSIONS
  Microglial cells migrate during CNS development and after CNS damage or disease. Thus, there are broad implications of the finding that classically and alternatively activated microglia differ in morphology, cytoskeleton, migratory and invasive capacity, and in the usage of ECM-degrading enzymes.
authors.affiliation: Toronto Western Research Institute, Room MC9-417, 399 Bathurst Street, Toronto, ON M5T 2S8, Canada.
authors.forename: Starlee
authors.initials: S
authors.lastname: Lively
authors.forename: Lyanne C
authors.initials: LC
authors.lastname: Schlichter
mesh_terms: D000199:Actins / Q000378:metabolism; D000818:Animals; D002403:Cathepsins / Q000378:metabolism; D002465:Cell Movement / Q000502:physiology*; D002478:Cells, Cultured; D003599:Cytoskeleton / Q000648:ultrastructure; D005109:Extracellular Matrix / Q000201:enzymology* / Q000502:physiology*; D005353:Fibronectins / Q000378:metabolism; D005966:Glucuronidase / Q000378:metabolism; D007150:Immunohistochemistry; D015847:Interleukin-4 / Q000096:biosynthesis / Q000235:genetics; D008070:Lipopolysaccharides / Q000494:pharmacology; D008262:Macrophage Activation / Q000502:physiology*; D020782:Matrix Metalloproteinases / Q000378:metabolism; D017628:Microglia / Q000502:physiology*; D022101:Microtubule-Organizing Center / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D060888:Real-Time Polymerase Chain Reaction; D016596:Vinculin / Q000378:metabolism; D014947:Wounds and Injuries / Q000473:pathology; D000099175:Heparanase
chemical_list: D000199:Actins; D002403:Cathepsins; D005353:Fibronectins; D005966:Glucuronidase; D015847:Interleukin-4; D008070:Lipopolysaccharides; D020782:Matrix Metalloproteinases; D016596:Vinculin; D000099175:Heparanase