title: Milk whey protein modification by coffee-specific phenolics: effect on structural and functional properties.
abstract: A suitable vehicle for integration of bioactive plant constituents is proposed. It involves modification of proteins using phenolics and applying these for protection of labile constituents. It dissects the noncovalent and covalent interactions of β-lactoglobulin with coffee-specific phenolics. Alkaline and polyphenol oxidase modulated covalent reactions were compared. Tryptic digestion combined with MALDI-TOF-MS provided tentative allocation of the modification type and site in the protein, and an in silico modeling of modified β-lactoglobulin is proposed. The modification delivers proteins with enhanced antioxidative properties. Changed structural properties and differences in solubility, surface hydrophobicity, and emulsification were observed. The polyphenol oxidase modulated reaction provides a modified β-lactoglobulin with a high antioxidative power, is thermally more stable, requires less energy to unfold, and, when emulsified with lutein esters, exhibits their higher stability against UV light. Thus, adaptation of this modification provides an innovative approach for functionalizing proteins and their uses in the food industry.
authors.affiliation: Institute of Nutritional Science, University of Potsdam, Nuthetal, Germany.
authors.forename: Mostafa
authors.initials: M
authors.lastname: Ali
authors.forename: Thomas
authors.initials: T
authors.lastname: Homann
authors.forename: Mahmoud
authors.initials: M
authors.lastname: Khalil
authors.forename: Hans-Peter
authors.initials: HP
authors.lastname: Kruse
authors.forename: Harshadrai
authors.initials: H
authors.lastname: Rawel
mesh_terms: D000818:Animals; D004156:Catechol Oxidase / Q000378:metabolism; D002417:Cattle; D003069:Coffee / Q000737:chemistry*; D004355:Drug Stability; D045703:Emulsifying Agents; D004655:Emulsions / Q000737:chemistry; D006358:Hot Temperature; D057927:Hydrophobic and Hydrophilic Interactions; D007782:Lactoglobulins / Q000737:chemistry* / Q000187:drug effects; D008892:Milk / Q000737:chemistry*; D008894:Milk Proteins / Q000737:chemistry*; D008958:Models, Molecular; D010636:Phenols / Q000494:pharmacology*; D011801:Quinic Acid / Q000031:analogs & derivatives / Q000494:pharmacology; D012995:Solubility; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014357:Trypsin / Q000378:metabolism; D000067816:Whey Proteins
chemical_list: D003069:Coffee; D045703:Emulsifying Agents; D004655:Emulsions; D007782:Lactoglobulins; D008894:Milk Proteins; D010636:Phenols; D000067816:Whey Proteins; C472707:caffeoylquinic acid; D011801:Quinic Acid; D004156:Catechol Oxidase; D014357:Trypsin