title: Probe ligation and real-time detection of KPC, OXA-48, VIM, IMP, and NDM carbapenemase genes.
abstract: The Check-MDR Carba test (Check-Points, Wageningen, Netherlands), which is based on specific molecular recognition of blaNDM, blaKPC, blaOXA-48, blaVIM, and blaIMP genes by DNA probe ligation and real-time PCR detection, was evaluated on 183 well-characterized Gram-negative rods. Representatives of the 5 gene families were accurately identified (specificities and sensitivities of 100%) within 4.5 hours. This test may be helpful to differentiate carbapenem resistance mediated by carbapenemases from those involving other mechanisms.
authors.affiliation: Service de Bactériologie-Virologie, INSERM U914: Emerging Resistance to Antibiotics, Hôpital de Bicêtre, Assistance Publique-Hôpitaux de Paris, Faculté de Médecine Paris-Sud, France.
authors.forename: G
authors.initials: G
authors.lastname: Cuzon
authors.forename: T
authors.initials: T
authors.lastname: Naas
authors.forename: P
authors.initials: P
authors.lastname: Bogaerts
authors.forename: Y
authors.initials: Y
authors.lastname: Glupczynski
authors.forename: P
authors.initials: P
authors.lastname: Nordmann
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D001681:Biological Assay / Q000592:standards*; D015780:Carbapenems / Q000494:pharmacology; D017931:DNA Primers / Q000235:genetics; D015342:DNA Probes / Q000235:genetics; D015870:Gene Expression; D006090:Gram-Negative Bacteria / Q000187:drug effects / Q000201:enzymology / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D007527:Isoenzymes / Q000145:classification / Q000235:genetics / Q000378:metabolism; D008826:Microbial Sensitivity Tests; D060888:Real-Time Polymerase Chain Reaction / Q000592:standards*; D012680:Sensitivity and Specificity; D018440:beta-Lactam Resistance / Q000235:genetics*; D001618:beta-Lactamases / Q000145:classification / Q000235:genetics* / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D015780:Carbapenems; D017931:DNA Primers; D015342:DNA Probes; D007527:Isoenzymes; D001618:beta-Lactamases; C063912:carbapenemase
keywords: Carbapenem resistance; Detection; Real-time PCR