title: The effects of low-level laser therapy on palatal mucoperiosteal wound healing and oxidative stress status in experimental diabetic rats.
abstract: OBJECTIVE
  The biostimulation effects of low-level laser therapy (LLLT) have recently been demonstrated. In this study, we aimed to investigate the effects of LLLT on palatal mucoperiostal wound healing and oxidative stress status in experimental diabetic rats.
  
  
  METHODS
  Forty-two male Wistar rats that weighed 250-300 g were used in this study. Experimental diabetes was induced in all of the rats using streptozotocin. A standardized full thickness wound was made in the mucoperiosteum of the hard palates of the rats using a 3 mm biopsy punch. The rats were divided into groups: 1 (control group, non- irradiated), and 2 (experimental group, irradiated). Treatment using a GaAlAs laser at a wavelength of 940 nm and at dose of 10 J/cm(2) began after surgery, and was repeated on the 2nd, 4th, and 6th days post-surgery. Seven animals from each group were killed on the 7th, 14th, and 21st day after surgery. Biopsies were performed for the histological analysis and blood samples were collected by cardiac puncture for biochemical analysis.
  
  
  RESULTS
  The histopathological findings revealed reduced numbers of inflammatory cells, and increased mitotic activity of fibroblasts, collagen synthesis, and vascularization in rats in group 2. The total oxidative status was significantly decreased in the laser-treated group on the 21st day.
  
  
  CONCLUSIONS
  LLLT elicits a positive healing effect on palatal mucoperiostal wounds, and modulates the oxidative status in experimental diabetic rats.
authors.affiliation: Department of Periodontology, Faculty of Dentistry, Dicle University, Diyarbakir, Turkey. elakadiroglu@hotmail.com
authors.forename: Ela Tules
authors.initials: ET
authors.lastname: Firat
authors.forename: Ahmet
authors.initials: A
authors.lastname: Dağ
authors.forename: Ahmet
authors.initials: A
authors.lastname: Günay
authors.forename: Beyza
authors.initials: B
authors.lastname: Kaya
authors.forename: Mehmet İrfan
authors.initials: Mİ
authors.lastname: Karadede
authors.forename: Berna Ersöz
authors.initials: BE
authors.lastname: Kanay
authors.forename: Aydin
authors.initials: A
authors.lastname: Ketani
authors.forename: Osman
authors.initials: O
authors.lastname: Evliyaoğlu
authors.forename: Ersin
authors.initials: E
authors.lastname: Uysal
mesh_terms: D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000503:physiopathology*; D028022:Low-Level Light Therapy; D008297:Male; D009061:Mouth Mucosa / Q000293:injuries* / Q000528:radiation effects; D018384:Oxidative Stress / Q000528:radiation effects*; D010159:Palate / Q000293:injuries* / Q000528:radiation effects; D010521:Periosteum / Q000293:injuries* / Q000528:radiation effects; D051381:Rats; D017208:Rats, Wistar; D014945:Wound Healing / Q000528:radiation effects*