title: Trends in polymeric delivery of nucleic acids to tumors.
abstract: Delivery of nucleic acids to tumors has received extensive attention in the past few decades since these molecules are capable of treating disease by modulating the source of abnormalities. Although high efficiency and low toxicity of numerous delivery systems for nucleic acids have been approved frequently with in vitro assays, contradictions have been observed in many cases between these results and what has occurred in the dynamic in vivo situation. Filling this gap seems to be crucial for further preclinical development of such systems. In this paper, we discuss various barriers which polymeric DNA or siRNA nanoparticles encounter upon systemic administration with an aim to assist in designing more relevant in vitro assays. Furthermore, individual considerations concerning delivery of DNA and siRNA have been addressed.
authors.affiliation: Department of Pharmaceutics, Utrecht Institute for Pharmaceutical Sciences, Faculty of Science, Utrecht University, Universiteitsweg 99, 3584 CG Utrecht, The Netherlands.
authors.forename: Afrouz
authors.initials: A
authors.lastname: Yousefi
authors.forename: Gert
authors.initials: G
authors.lastname: Storm
authors.forename: Raymond
authors.initials: R
authors.lastname: Schiffelers
authors.forename: Enrico
authors.initials: E
authors.lastname: Mastrobattista
mesh_terms: D000818:Animals; D004247:DNA / Q000008:administration & dosage* / Q000737:chemistry; D018014:Gene Transfer Techniques; D006801:Humans; D009369:Neoplasms / Q000628:therapy*; D011108:Polymers / Q000008:administration & dosage* / Q000737:chemistry; D034741:RNA, Small Interfering / Q000008:administration & dosage* / Q000737:chemistry
chemical_list: D011108:Polymers; D034741:RNA, Small Interfering; D004247:DNA
keywords: Cancer; Formulation; Gene delivery; Nanocarriers; Polyplexes