title: Real-time monitoring of DNA hybridization kinetics on living cell surfaces.
abstract: We determined hybridization rates of DNA probes bound to the surfaces of a human cell, and compared the rates with those determined in solution. The rates were slower on the cell surface than in solution-phase. The position of the nucleation site but not the location of the formed duplex relative to the cell surface adversely affected the hybridization kinetics.
authors.affiliation: Frontier Institute for Biomolecular Engineering Research (FIBER), Konan University, 7-1-20 Minatojima-minamimachi, Kobe 650-0047, Japan.
authors.forename: Ambadas B
authors.initials: AB
authors.lastname: Rode
authors.forename: Tamaki
authors.initials: T
authors.lastname: Endoh
authors.forename: Hisae
authors.initials: H
authors.lastname: Tateishi-Karimata
authors.forename: Shuntaro
authors.initials: S
authors.lastname: Takahashi
authors.forename: Naoki
authors.initials: N
authors.lastname: Sugimoto
mesh_terms: D004247:DNA / Q000032:analysis* / Q000737:chemistry*; D015342:DNA Probes / Q000032:analysis / Q000737:chemistry; D018922:HL-60 Cells; D006801:Humans; D007700:Kinetics; D009693:Nucleic Acid Hybridization; D013499:Surface Properties; D013997:Time Factors
chemical_list: D015342:DNA Probes; D004247:DNA