title: E-cadherin downregulation and Twist overexpression since early stages of oral carcinogenesis.
abstract: There is some evidence of Twist participation in oral carcinogenesis; however, little is known about its interaction with E-cadherin in oral squamous cell carcinoma (OSCC) development. This experimental study included an immunohistochemical analysis of Twist and E-cadherin proteins in paraffin-embedded specimens of oral leukoplakia (OL), OSCC, and normal oral mucosa. In addition, it was also performed a Western blot and double-immunofluorescence analysis of Twist and E-cadherin expression in OSCC cell lines. Significant differences in Twist and E-cadherin immunoexpression were observed between normal oral mucosa and OL, with an inverse relation since the earliest stages of oral dysplasia (r = -0,512; P < 0.001). Western blot and double-immunofluorescence analysis showed differences in Twist and E-cadherin expression among human oral keratinocytes and OSCC cell lines suggesting that downregulation of E-cadherin occurs in a dependent manner of Twist in OSCC. Our results showed a possible value of Twist and E-cadherin in the prediction of risk of oral epithelium malignant transformation.
authors.affiliation: Departament of Oral Pathology, Dental School, University of São Paulo, São Paulo, Brazil.
authors.forename: Brunno Santos
authors.initials: BS
authors.lastname: de Freitas Silva
authors.forename: Fernanda Paula
authors.initials: FP
authors.lastname: Yamamoto-Silva
authors.forename: Hélder Antônio Rebelo
authors.initials: HA
authors.lastname: Pontes
authors.forename: Décio dos Santos
authors.initials: Ddos S
authors.lastname: Pinto Júnior
mesh_terms: D015153:Blotting, Western; D015820:Cadherins / Q000032:analysis*; D063646:Carcinogenesis / Q000473:pathology*; D002294:Carcinoma, Squamous Cell / Q000473:pathology; D018929:Cell Culture Techniques; D045744:Cell Line, Tumor; D002471:Cell Transformation, Neoplastic / Q000473:pathology; D003593:Cytoplasm / Q000473:pathology; D018450:Disease Progression; D015536:Down-Regulation; D004848:Epithelium / Q000473:pathology; D005455:Fluorescent Antibody Technique; D005544:Forecasting; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D015603:Keratinocytes / Q000473:pathology; D007972:Leukoplakia, Oral / Q000473:pathology; D009061:Mouth Mucosa / Q000473:pathology; D009062:Mouth Neoplasms / Q000473:pathology*; D009687:Nuclear Proteins; D011230:Precancerous Conditions / Q000473:pathology; D051879:Twist-Related Protein 1
chemical_list: D015820:Cadherins; D009687:Nuclear Proteins; C103173:TWIST1 protein, human; D051879:Twist-Related Protein 1
keywords: E-cadherin; Twist; oral cancer; oral dysplasia; oral leukoplakia; oral squamous cell carcinoma