title: The expression of Heparanase mRNA and its clinical significance in Hepatocellular carcinoma.
abstract: OBJECTIVE
  To investigate the clinico-pathologic importance of Heparanase mRNA expression in hepatocellular carcinoma and its relationship with prognosis.
  
  
  METHODS
  Fresh tumors and noncancerous liver tissues were obtained from 30 hepatocellular carcinoma patients after hepatectomy. Expression levels of Heparanase and glyceraldehyde-3-phosphate dehydrogenase mRNA were quantitatively analyzed by real-time reverse transcriptase-polymerase chain reaction.
  
  
  RESULTS
  The relative Heparanase mRNA expression level (Heparanase: glyceraldehyde-3-phosphate dehydrogenase ratio) in hepatocellular carcinoma was lower than that in noncancerous tissue (P < 0.05).
  
  
  CONCLUSIONS
  Reduced Heparanase mRNA expression might result in abnormal cell growth and correlate with hepatocellular carcinoma progression.
authors.affiliation: Department of General Surgery, Jiangsu Shengze Hospital, Wujiang, Jiangsu, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Wang
authors.forename: Yu
authors.initials: Y
authors.lastname: Shen
authors.forename: Peng
authors.initials: P
authors.lastname: Wang
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Li
mesh_terms: D000328:Adult; D000368:Aged; D006528:Carcinoma, Hepatocellular / Q000201:enzymology / Q000235:genetics* / Q000473:pathology / Q000601:surgery; D018450:Disease Progression; D005260:Female; D005966:Glucuronidase / Q000235:genetics* / Q000378:metabolism; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000235:genetics / Q000378:metabolism; D006801:Humans; D008113:Liver Neoplasms / Q000201:enzymology / Q000235:genetics* / Q000473:pathology / Q000601:surgery; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000235:genetics; D000099175:Heparanase
chemical_list: D005966:Glucuronidase; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; D012333:RNA, Messenger; D000099175:Heparanase