title: Immunolocalization of aquaporin-10 in tuberculous human ileum.
abstract: OBJECTIVE
  To determine the presence of AQP-10 in the ileum of patients suffering from intestinal tuberculosis.
  
  
  METHODS
  A cross-sectional analytical study.
  
  
  METHODS
  Department of Anatomy, University of Health Sciences, Lahore, in year 2010.
  
  
  METHODS
  Thirty seven paraffin embedded blocks of either surgically resected specimens or ileal biopsies with diagnosis of intestinal tuberculosis were selected from records of the histopathology departments of local hospitals. These cases were subdivided into two groups: A-1 (with tuberculous granulomatous lesions with or without epithelium) and A-2 (without tuberculous lesion lying adjacent to the lesions and having an intact epithelium). Specimens of small intestine with malignancy, Crohn's disease, inflammatory bowel disease, irritable bowel syndrome and diarrhoeal diseases caused by Rota virus, adenovirus, Salmonella, Shigella and Escherichia coli were excluded. The variables studied were the presence/absence and location of AQP-10.
  
  
  RESULTS
  The most common clinical symptoms found in tuberculous patients were abdominal pain followed by diarrhoea. A significant association was found between AQP-10 and site of granulomas and caseation necrosis (p=0.002 and p=0.006 respectively). Absence of AQP-10 was observed in tuberculous ileum at the site of lesion with ulceration. A strong positive staining of AQP-10 was found in the intact epithelium at sites adjacent to the tuberculous lesion indicating its localization near the epithelial lining of ileum.
  
  
  CONCLUSIONS
  AQP-10 was present only on the epithelial cells occurring at the luminal side of the villi and was absent in tuberculous ileum where epithelium was absent.
authors.affiliation: Department of Anatomy, Centre Park Medical College, Lahore.
authors.forename: Tahrim
authors.initials: T
authors.lastname: Ansar
authors.forename: Muhammad
authors.initials: M
authors.lastname: Tahir
authors.forename: Khalid Pervez
authors.initials: KP
authors.lastname: Lone
authors.forename: Bushra
authors.initials: B
authors.lastname: Munir
mesh_terms: D015746:Abdominal Pain / Q000209:etiology; D000328:Adult; D020346:Aquaporins / Q000378:metabolism*; D001706:Biopsy; D003430:Cross-Sectional Studies; D003967:Diarrhea / Q000209:etiology; D005260:Female; D006801:Humans; D007082:Ileum / Q000378:metabolism / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D014385:Tuberculosis, Gastrointestinal / Q000473:pathology*
chemical_list: D020346:Aquaporins