title: 5-Hydroxytryptamine levels in the pulmonary arterioles of broilers with induced pulmonary hypertension and its relationship to pulmonary vascular remodelling.
abstract: This experiment was performed to explore the relationship between 5-hydroxytryptamine (5-HT) levels in pulmonary arterioles and in pulmonary vascular remodelling in broilers. Pulmonary arterial hypertension was induced by injecting cellulose microparticles intravenously. Pulmonary hypertension syndrome (PHS) morbidity, right ventricle/total ventricle weight ratio (RV/TV), packed cell volume (PCV), haemoglobin concentration (HB), vessel wall area to vessel total area ratio (WA/TA) and mean tunica media thickness in pulmonary arterioles (mMTPA) were measured. Proliferating cell nuclear antigen (PCNA), argyrophilic nucleolar organizer region proteins (Ag-NORs) and 5-HT content in pulmonary arterioles were determined. The results showed that injecting cellulose microparticles intravenously in broilers could successfully increase the PHS morbidity, significantly elevate RV/TV, PCV and HB, significantly increase mMTPA and WA/TA, and significantly increase the argyrophilic particles in smooth muscle cell nucleoli, PCNA-positive cells in the medial layer, and the 5-HT content in pulmonary arterioles. Correlation analysis showed that the level of 5-HT was strongly positively correlated with PCNA and Ag-NORs. The results indicated that the increase of 5-HT in the tunica media could possibly promote the proliferation of smooth muscle cells in pulmonary arterioles and thus the occurrence of pulmonary vascular remodelling.
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, Guangzhou, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Li
authors.forename: Jian-Ying
authors.initials: JY
authors.lastname: Zeng
authors.forename: Zhao-Xin
authors.initials: ZX
authors.lastname: Tang
authors.forename: Yu-Gu
authors.initials: YG
authors.lastname: Li
authors.forename: Jian-Ying
authors.initials: JY
authors.lastname: Guo
authors.forename: Jia-Qiang
authors.initials: JQ
authors.lastname: Pan
mesh_terms: D000818:Animals; D001160:Arterioles / Q000378:metabolism*; D002482:Cellulose / Q000008:administration & dosage; D002645:Chickens; D006400:Hematocrit; D006454:Hemoglobins / Q000378:metabolism; D006976:Hypertension, Pulmonary / Q000378:metabolism / Q000662:veterinary*; D008168:Lung / Q000098:blood supply*; D018919:Neovascularization, Physiologic / Q000502:physiology*; D011201:Poultry Diseases / Q000378:metabolism*; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D012701:Serotonin / Q000378:metabolism*; D017540:Tunica Media / Q000378:metabolism
chemical_list: D006454:Hemoglobins; D018809:Proliferating Cell Nuclear Antigen; D012701:Serotonin; D002482:Cellulose