title: Protein encapsulation in biodegradable polymeric nanoparticles: morphology, fluorescence behaviour and stem cell uptake.
abstract: The synthesis and characterization of new biodegradable polymeric NPs loaded with bovine serum albumin marked with fluorescein isothiocyanate (FITC-BSA) is reported. The protein is encapsulated in poly(D,L-lactide-co-glycolide) (PLGA) NPs by the double emulsion method with subsequent solvent evaporation. The NPs display a spherical shape with a narrow size distribution and no aggregation is observed after drying. Steady-state and time-resolved fluorescence measurements appear to be a sensitive method to investigate the protein environment on the nanometer-scale. Finally, FITC-BSA-loaded NPs are rapidly internalized in stem cells. Interestingly, 25% cells were slightly positive after 28 days.
authors.affiliation: Materials Engineering Center, UdR INSTM, University of Perugia, Str. Pentima 4, 05100, Terni, Italy; Institute of Polymer Science and Technology, CSIC, Juan de la Cierva 3, 28006, Madrid, Spain.
authors.forename: Nicoletta
authors.initials: N
authors.lastname: Rescignano
authors.forename: Luigi
authors.initials: L
authors.lastname: Tarpani
authors.forename: Roberto
authors.initials: R
authors.lastname: Tiribuzi
authors.forename: Simona
authors.initials: S
authors.lastname: Montesano
authors.forename: Sabata
authors.initials: S
authors.lastname: Martino
authors.forename: Loredana
authors.initials: L
authors.lastname: Latterini
authors.forename: Josè Maria
authors.initials: JM
authors.lastname: Kenny
authors.forename: Ilaria
authors.initials: I
authors.lastname: Armentano
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology*; D001673:Biodegradation, Environmental; D001854:Bone Marrow Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D002152:Calorimetry, Differential Scanning; D002417:Cattle; D002478:Cells, Cultured; D016650:Fluorescein-5-isothiocyanate / Q000031:analogs & derivatives / Q000378:metabolism; D006801:Humans; D055767:Immobilized Proteins / Q000378:metabolism*; D019344:Lactic Acid / Q000737:chemistry*; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D012710:Serum Albumin, Bovine / Q000378:metabolism; D013050:Spectrometry, Fluorescence; D013696:Temperature; D013818:Thermogravimetry
chemical_list: D001672:Biocompatible Materials; D055767:Immobilized Proteins; C039217:fluorescein isothiocyanate bovine serum albumin; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D012710:Serum Albumin, Bovine; D019344:Lactic Acid; D016650:Fluorescein-5-isothiocyanate
keywords: PLGA; fluorescence; nanoparticles; stem cells