title: Synthesis and characterization of fumaric acid functionalized AgCl/titania nanocomposite with enhanced antibacterial activity.
abstract: Silver based antimicrobial agents with TiO2 as a host support (AgCl/TiO2) are increasingly being evaluated for disinfection and therapeutic applications. In this work TiO2 supported silver chloride nanocomposites were synthesized and functionalized with fumaric acid. X-ray diffraction results showed that the TiO2 was in anatase phase and AgCI was in the cubic chalcoargyrite phase. The functionalization was confirmed by FTIR analysis. The fumaric acid functionalized AgCl/TiO2 (Fu-AgCl/TiO2) showed uniform particle size distribution in the range of 4-5 nm. The intensity size distribution of Fu-AgCl/TiO2 nanocomposite by DLS showed an average particle diameter of -290 nm with a polydispersity index (P. I.) of 0.47. Further, a high BET surface area of -320.7 m2/g was observed for Fu-AgCl/TiO2 with an average pore size distribution of 3.8 nm. The antimicrobial activity of Fu-AgCl/TiO2 and AgCl/TiO2 was evaluated by determining the MIC and MBC values, and it was observed that Fu-AgCl/TiO2 exhibited better antimicrobial activity as compared to the unfunctionalized nanocomposite. The antimicrobial activity of Fu-AgCl/TiO2 is mainly attributed to its superior physicochemical properties coupled with the synergistic action of both fumaric acid and silver ions. The changes in the cytoplasmic membrane of the cells due to the formation of intracellular ROS were observed by SEM imaging.
authors.affiliation: Department of Biological Sciences, Birla Institute of Technology and Science Pilani, K K Birla Goa Campus, Zuarinagar, Goa 403726, India.
authors.forename: Vilas
authors.initials: V
authors.lastname: Desai
authors.forename: Meenal
authors.initials: M
authors.lastname: Kowshik
mesh_terms: D000900:Anti-Bacterial Agents / Q000138:chemical synthesis / Q000494:pharmacology*; D005650:Fumarates / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D053761:Nanocomposites; D018030:Silver Compounds / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D014025:Titanium / Q000737:chemistry*; D014961:X-Ray Diffraction
chemical_list: D000900:Anti-Bacterial Agents; D005650:Fumarates; D018030:Silver Compounds; C032005:fumaric acid; D014025:Titanium; C037548:silver chloride