title: Engineering Chinese hamster ovary (CHO) cells for producing recombinant proteins with simple glycoforms by zinc-finger nuclease (ZFN)-mediated gene knockout of mannosyl (alpha-1,3-)-glycoprotein beta-1,2-N-acetylglucosaminyltransferase (Mgat1).
abstract: While complex N-linked glycoforms are often desired in biotherapeutic protein production, proteins with simple, homogeneous glycan structure have implications for X-ray crystallography and for recombinant therapeutics targeted to the mannose receptor of antigen presenting cells. Mannosyl (alpha-1,3-)-glycoprotein beta-1,2-N-acetylglucosaminyltransferase (Mgat1, also called GnTI) adds N-acetylglucosamine to the Man5GlcNAc2 (Man5) N-glycan structure as part of complex N-glycan synthesis. Here, we report the use of zinc-finger nuclease (ZFN) genome editing technology to create Mgat1 disrupted Chinese hamster ovary (CHO) cell lines. These cell lines allow for the production of recombinant proteins with Man5 as the predominant N-linked glycosylation species. This method provides advantages over previously reported methods to create Mgat1-deficient cell lines. The use of ZFN-based genome editing eliminates potential regulatory concerns associated with random chemical mutagenesis, while retaining the robust growth and productivity characteristics of the parental cell lines. These Mgat1 disrupted cell lines may be used to produce mannose receptor-targeted therapeutic proteins. Cell line generation work can be performed in both Mgat1 disrupted and wild-type host cell lines to conduct X-ray crystallography studies of protein therapeutics in the same cell line used for production.
authors.affiliation: Cell Sciences and Development, SAFC/Sigma-Aldrich, 2909 Laclede Avenue, St. Louis, MO 63103, USA. Natalie.sealover@sial.com
authors.forename: Natalie R
authors.initials: NR
authors.lastname: Sealover
authors.forename: Angela M
authors.initials: AM
authors.lastname: Davis
authors.forename: Jeanne K
authors.initials: JK
authors.lastname: Brooks
authors.forename: Henry J
authors.initials: HJ
authors.lastname: George
authors.forename: Kevin J
authors.initials: KJ
authors.lastname: Kayser
authors.forename: Nan
authors.initials: N
authors.lastname: Lin
mesh_terms: D000818:Animals; D016466:CHO Cells; D006224:Cricetinae; D003412:Cricetulus; D004722:Endoribonucleases / Q000235:genetics*; D055786:Gene Knockout Techniques; D005974:Glutamate-Ammonia Ligase / Q000235:genetics; D007074:Immunoglobulin G / Q000378:metabolism; D017351:N-Acetylglucosaminyltransferases / Q000235:genetics*; D011994:Recombinant Proteins; D014157:Transcription Factors / Q000235:genetics*
chemical_list: D007074:Immunoglobulin G; D011994:Recombinant Proteins; D014157:Transcription Factors; C561128:MGAT1 protein, human; D017351:N-Acetylglucosaminyltransferases; D004722:Endoribonucleases; D005974:Glutamate-Ammonia Ligase
keywords: CHO cells; Glycoengineering; High mannose; Mgat1; Zinc-finger nuclease