title: Tanshinone IIA and Baicalin inhibiting the formation of benzo[a]pyrene and benzo[a]pyrene induced cytotoxicity: correlation with scavenging free radical.
abstract: Benzo[a]pyrene (BaP), a ubiquitous environmental pollutant, is formed by incomplete combustion of organic materials, and causes oxidative damage to cells and tissues due to reactive oxygen species (ROS). The purpose of this study is to investigate the inhibition of Tanshinone IIA and Baicalin on the formation of BaP as well as the cytotoxicity in human umbilical vein endothelial cells (HUVECs) induced by BaP. The results showed that BaP formations in mainstream smoke were inhibited by 21μg/cigarette of Tanshinone IIA with a 12.8% decrease, and by 60μg/cigarette of Baicalin with an 11.1% decrease, respectively. Tanshinone IIA could protect HUVECs from the damage caused by BaP in a dose-dependent manner from 7.5 to 30μg/ml. 6μg/ml Baicalin significantly increased the cell survival rate from 47.37% to 84.21% compared with BaP-treated cells. Both Tanshinone IIA and Baicalin markedly attenuated the increase of LDH release, enhanced the activity of SOD and GPx and inhibited the generation of MDA in BaP-damaged HUVECs in vitro. In vivo exploration showed that the two compounds were capable of enhancing the activity of SOD and inhibiting generation of MDA in mainstream smoke-damaged in mice. Superoxide anion radical and hydroxyl radical were obviously inhibited by Tanshinone IIA and Baicalin. These data demonstrate an inhibition of Tanshinone IIA and Baicalin on the formation of BaP and the cytotoxicity on HUVECs related to free Radical induced by BaP.
authors.affiliation: Department of Pharmacology, School of Medicine, Xi'an Jiaotong University, Xi'an 710061, PR China; Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education/College of Life Science, Northwest University, Xi'an 710069, PR China.
authors.forename: Shixiang
authors.initials: S
authors.lastname: Wang
authors.affiliation: Department of Pharmacology, School of Medicine, Xi'an Jiaotong University, Xi'an 710061, PR China. Electronic address: zwj@xjtu.edu.cn.
authors.forename: Weijin
authors.initials: W
authors.lastname: Zang
authors.affiliation: Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education/College of Life Science, Northwest University, Xi'an 710069, PR China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education/College of Life Science, Northwest University, Xi'an 710069, PR China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Department of Pharmacology, School of Medicine, Xi'an Jiaotong University, Xi'an 710061, PR China.
authors.forename: Ming
authors.initials: M
authors.lastname: Zhao
authors.affiliation: Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education/College of Life Science, Northwest University, Xi'an 710069, PR China.
authors.forename: Zhijuan
authors.initials: Z
authors.lastname: Gao
authors.affiliation: Tianjin Cigarette Factory, Shanghai Tobacco Group Corporation, Tianjin 300163, PR China.
authors.forename: Gangcheng
authors.initials: G
authors.lastname: Li
authors.affiliation: Tianjin Cigarette Factory, Shanghai Tobacco Group Corporation, Tianjin 300163, PR China.
authors.forename: Qingnai
authors.initials: Q
authors.lastname: Meng
authors.affiliation: Shaanxi Provincial People's Hospital, Xi'an 710068, PR China.
authors.forename: Qinshe
authors.initials: Q
authors.lastname: Liu
authors.affiliation: Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education/College of Life Science, Northwest University, Xi'an 710069, PR China. Electronic address: zhengxh@nwu.edu.cn.
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Zheng
mesh_terms: D045784:Abietanes / Q000494:pharmacology*; D000818:Animals; D001564:Benzo(a)pyrene / Q000378:metabolism / Q000633:toxicity*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D019610:Cytoprotection; D004305:Dose-Response Relationship, Drug; D005419:Flavonoids / Q000494:pharmacology*; D016166:Free Radical Scavengers / Q000494:pharmacology*; D005979:Glutathione Peroxidase / Q000378:metabolism; D061307:Human Umbilical Vein Endothelial Cells / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D006801:Humans; D017665:Hydroxyl Radical / Q000378:metabolism; D019570:Inhalation Exposure; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008315:Malondialdehyde / Q000378:metabolism; D051379:Mice; D008813:Mice, Inbred ICR; D012906:Smoke / Q000009:adverse effects* / Q000032:analysis; D012907:Smoking / Q000009:adverse effects* / Q000378:metabolism; D052616:Solid Phase Extraction; D013482:Superoxide Dismutase / Q000378:metabolism; D013481:Superoxides / Q000378:metabolism
chemical_list: D045784:Abietanes; D005419:Flavonoids; D016166:Free Radical Scavengers; D012906:Smoke; C021751:tanshinone; D013481:Superoxides; D017665:Hydroxyl Radical; D001564:Benzo(a)pyrene; C038044:baicalin; D008315:Malondialdehyde; D007770:L-Lactate Dehydrogenase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase
keywords: Baicalin; Benzo[a]pyrene; Free radical; Tanshinone IIA