title: Inhibitory Effect of Arctigenin from Fructus Arctii Extract on Melanin Synthesis via Repression of Tyrosinase Expression.
abstract: To identify the active compound arctigenin in Fructus Arctii (dried seed of medicinal plant Arctium lappa) and to elucidate the inhibitory mechanism in melanogenesis, we analyzed melanin content and tyrosinase activity on B16BL6 murine melanoma and melan-A cell cultures. Water extracts of Fructus Arctii were shown to inhibit tyrosinase activity in vitro and melanin content in α -melanocyte stimulating hormone-stimulated cells to similar levels as the well-known kojic acid and arbutin, respectively. The active compound arctigenin of Fructus Arctii displayed little or no cytotoxicity at all concentrations examined and decreased the relative melanin content and tyrosinase activity in a dose-dependent manner. Melanogenic inhibitory activity was also identified in vivo with zebrafish embryo. To determine the mechanism of inhibition, the effects of arctigenin on tyrosinase gene expression and tyrosinase promoter activity were examined. Also in addition, in the signaling cascade, arctigenin dose dependently decreased the cAMP level and promoted the phosphorylation of extracellular signal-regulated kinase. This result suggests that arctigenin downregulates cAMP and the tyrosinase enzyme through its gene promoter and subsequently upregulates extracellular signal-regulated kinase activity by increasing phosphorylation in the melanogenesis signaling pathway, which leads to a lower melanin content.
authors.affiliation: KM-Based Herbal Drug Research Group, Korea Institute of Oriental Medicine, Yuseong-Daero 1672, Yuseong-Gu, Daejeon 305-811, Republic of Korea.
authors.forename: Hwayong
authors.initials: H
authors.lastname: Park
authors.forename: Kwang Hoon
authors.initials: KH
authors.lastname: Song
authors.forename: Pil Mun
authors.initials: PM
authors.lastname: Jung
authors.forename: Ji-Eun
authors.initials: JE
authors.lastname: Kim
authors.forename: Hyunju
authors.initials: H
authors.lastname: Ro
authors.forename: Mi Yoon
authors.initials: MY
authors.lastname: Kim
authors.forename: Jin Yeul
authors.initials: JY
authors.lastname: Ma