title: Identification of pVHL as a novel substrate for Aurora-A in clear cell renal cell carcinoma (ccRCC).
abstract: Clear cell renal cell carcinoma (ccRCC) is the most common histological subtype of kidney cancer and is often characterized by mutations or deletions of the Von Hippel Lindau (VHL) tumour suppressor gene. Aurora gene family members are implicated in proper mitotic progression and spindle checkpoint function and play a crucial role in cancer progression. In the present study, we assessed the expression of Aurora-A in a cohort of 30 ccRCC with fully characterized VHL status (wt/wt or mut/del) and Fuhrman grade. Aurora-A transcript and protein levels were significantly increased in high Fuhrman grade tumours and in VHLwt/wt tumours. These results suggest that Aurora-A and VHL interact in the ccRCC. We demonstrated that the two proteins interact in vivo and identified the Ser72 on the sequence of VHL as the unique site phosphorylated by Aurora-A.
authors.affiliation: CNRS - UMR 6290 (IGDR) -Université Rennes 1- BIOSIT - Rennes, France.
authors.forename: Benedicte
authors.initials: B
authors.lastname: Martin
authors.forename: Franck
authors.initials: F
authors.lastname: Chesnel
authors.forename: Jean-Guy
authors.initials: JG
authors.lastname: Delcros
authors.forename: Florence
authors.initials: F
authors.lastname: Jouan
authors.forename: Anne
authors.initials: A
authors.lastname: Couturier
authors.forename: Frederic
authors.initials: F
authors.lastname: Dugay
authors.forename: Xavier
authors.initials: X
authors.lastname: Le Goff
authors.forename: Jean-Jacques
authors.initials: JJ
authors.lastname: Patard
authors.forename: Patricia
authors.initials: P
authors.lastname: Fergelot
authors.forename: Cecile
authors.initials: C
authors.lastname: Vigneau
authors.forename: Nathalie
authors.initials: N
authors.lastname: Rioux-Leclerq
authors.forename: Yannick
authors.initials: Y
authors.lastname: Arlot-Bonnemains
mesh_terms: D064096:Aurora Kinase A / Q000235:genetics / Q000378:metabolism*; D002292:Carcinoma, Renal Cell / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007680:Kidney Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D008954:Models, Biological; D009154:Mutation; D060787:Neoplasm Grading; D010766:Phosphorylation; D011485:Protein Binding; D014158:Transcription, Genetic; D051794:Von Hippel-Lindau Tumor Suppressor Protein / Q000235:genetics / Q000378:metabolism*
chemical_list: D051794:Von Hippel-Lindau Tumor Suppressor Protein; D064096:Aurora Kinase A