title: An integrated proteomic approach to decipher the effect of methyl jasmonate elicitation on the proteome of Silybum marianum L. hairy roots.
abstract: Jasmonate and its methyl derivative, methyl jasmonate (MeJA), are naturally occurring compounds that mediate several plant physiological processes in response to pathogen attack, wounding, and ozone. Exogenous application of jasmonates triggers defense responses that resemble those initiated by pathogen infection and also modulates the production of certain secondary metabolites in a variety of plant species. In this study, we treated the hairy root cultures of Silybum marianum L. with 100 μM MeJA and then measured the content of Silymarin (SLM). We observed that the SLM content increased significantly after 48 h of MeJA treatment and remained constant for 120 h. However, MeJA treatment caused a significant growth reduction after 96 h incubation. The activity of lipoxygenase as a key enzyme in the jasmonate biosynthesis pathway and anti-oxidative enzymes; peroxidase and ascorbate peroxidase was also significantly increased after MeJA treatment. To elucidate the global effect of jasmonate on gene expression of S. marianum, we employed high resolution two-dimensional gel electrophoresis coupled with tandem mass spectrometry. Out of 670 reproducibly detected protein spots which were analyzed on each given gel, 32 spots were up- or down regulated upon MeJA treatment. Of them, ten proteins such as ER binding protein, glutamine synthetase, pathogenesis-related protein, caffeoyl CoA O-methyltransferase, and profilin-1 could be identified by mass spectrometry analysis. The possible implications of the identified proteins on physiological outcome of MeJA application in S. marianum hairy root culture will be discussed.
authors.affiliation: Department of Systems Biology, Agricultural Biotechnology Research Institute of Iran, Karaj, Iran. jgharechahi@gmail.com
authors.forename: Javad
authors.initials: J
authors.lastname: Gharechahi
authors.forename: Masumeh
authors.initials: M
authors.lastname: Khalili
authors.forename: Tahereh
authors.initials: T
authors.lastname: Hasanloo
authors.forename: Ghasem Hosseini
authors.initials: GH
authors.lastname: Salekdeh
mesh_terms: D000085:Acetates / Q000378:metabolism* / Q000494:pharmacology; D000975:Antioxidants / Q000378:metabolism; D060387:Ascorbate Peroxidases / Q000378:metabolism; D003517:Cyclopentanes / Q000378:metabolism* / Q000494:pharmacology; D015870:Gene Expression / Q000187:drug effects*; D008084:Lipoxygenase / Q000378:metabolism; D020944:Silybum marianum / Q000187:drug effects / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D054883:Oxylipins / Q000378:metabolism* / Q000494:pharmacology; D009195:Peroxidase / Q000378:metabolism; D063245:Plant Development / Q000187:drug effects; D010937:Plant Growth Regulators / Q000378:metabolism* / Q000494:pharmacology; D010940:Plant Proteins / Q000378:metabolism*; D018517:Plant Roots / Q000187:drug effects / Q000378:metabolism*; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics / Q000379:methods; D012838:Silymarin / Q000378:metabolism
chemical_list: D000085:Acetates; D000975:Antioxidants; D003517:Cyclopentanes; D054883:Oxylipins; D010937:Plant Growth Regulators; D010940:Plant Proteins; D020543:Proteome; D012838:Silymarin; C072239:methyl jasmonate; D060387:Ascorbate Peroxidases; D009195:Peroxidase; D008084:Lipoxygenase
keywords: 2-DE; APX; CBB; FAME; GC; HPLC; Hairy root culture; IEF; ISB; IgG; JA; LOX; MALDI-TOF/TOF; MS; Mass spectrometry; MeJA; Methyl jasmonate (MeJA); PMF; POD; SB; SC; SD; SDS-PAGE; SLM; Silymarin (SLM); TXF; Two-dimensional gel electrophoresis; ascorbate peroxidase; coomassie brilliant blue; fatty acid methyl esters; gas chromatography; high-performance liquid chromatography; immune globulin G; isoelectric focusing; isosilybinin; jasmonic acid; lipoxygenase; mass spectrometry; matrix assisted laser desorption ionization-time of flight–time of flight; methyl jasmonate; peptide mass finger printing; peroxidase; silybinin; silycristin; silydianin; silymarin; sodium dodecyl sulfate-poly acrylamide gel electrophoresis; taxifolin; two-dimensional gel electrophoresis