title: GreenLight Model 960.
abstract: The importance of food safety has resulted in a demand for a more rapid, high-throughput method for total viable count (TVC). The industry standard for TVC determination (ISO 4833:2003) is widely used but presents users with some drawbacks. The method is materials- and labor-intensive, requiring multiple agar plates per sample. More importantly, the method is slow, with 72 h typically required for a definitive result. Luxcel Biosciences has developed the GreenLight Model 960, a microtiter plate-based assay providing a rapid high-throughput method of aerobic bacterial load assessment through analysis of microbial oxygen consumption. Results are generated in 1-12 h, depending on microbial load. The mix and measure procedure allows rapid detection of microbial oxygen consumption and equates oxygen consumption to microbial load (CFU/g), providing a simple, sensitive means of assessing the microbial contamination levels in foods (1). As bacteria in the test sample grow and respire, they deplete O2, which is detected as an increase in the GreenLight probe signal above the baseline level (2). The time required to reach this increase in signal can be used to calculate the CFU/g of the original sample, based on a predetermined calibration. The higher the initial microbial load, the earlier this threshold is reached (1).
authors.affiliation: Luxcel Biosciences Ltd, Suite 3.32, Bio Transfer Unit, UCC, Cork, Ireland.
authors.forename: Richard
authors.initials: R
authors.lastname: Fernandes
authors.forename: Conn
authors.initials: C
authors.lastname: Carey
authors.forename: James
authors.initials: J
authors.lastname: Hynes
authors.forename: Dmitri
authors.initials: D
authors.lastname: Papkovsky
mesh_terms: D000818:Animals; D001419:Bacteria / Q000302:isolation & purification; D001431:Bacteriological Techniques / Q000379:methods* / Q000592:standards*; D005516:Food Microbiology / Q000379:methods* / Q000592:standards*; D008460:Meat / Q000382:microbiology; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity