title: Optimisation of ultrasonic-assisted extraction of antioxidant compounds from Artemisia absinthium using response surface methodology.
abstract: Response surface methodology was used to optimise experimental conditions for ultrasonic-assisted extraction of phenolic compounds from Artemisia absinthium. The central composite design was employed, the extracts were characterised by the determination of total phenolic content and antioxidant capacity. The total phenolic contents of extracts were determined by Folin method and also total antioxidant capacities of extracts were determined by ABTS and CUPRAC methods. The phenolic compounds of A. absinthium at optimum extraction conditions were determined by HPLC-DAD. The optimum conditions were determined as HCl concentration between 0.41 and 0.44mol/L, methanol volume between 55% and 59% (v/v), extraction temperature between 64 and 70°C, extraction time between 101 and 107min. The experimental values agreed with those predicted within a 95% confidence level, thus indicating the suitability of response surface methodology in optimising the ultrasound-assisted extraction of phenolic compounds from A. absinthium.
authors.affiliation: University of Uludag, Faculty of Science and Arts, Department of Chemistry, 16059 Bursa, Turkey. salihabilgi@uludag.edu.tr
authors.forename: Saliha
authors.initials: S
authors.lastname: Sahin
authors.forename: Onder
authors.initials: O
authors.lastname: Aybastıer
authors.forename: Esra
authors.initials: E
authors.lastname: Işık
mesh_terms: D000975:Antioxidants / Q000032:analysis / Q000302:isolation & purification*; D031171:Artemisia absinthium / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D005638:Fruit / Q000737:chemistry*; D010636:Phenols / Q000032:analysis / Q000302:isolation & purification*; D010936:Plant Extracts / Q000032:analysis / Q000302:isolation & purification*; D014465:Ultrasonics / Q000379:methods*
chemical_list: D000975:Antioxidants; D010636:Phenols; D010936:Plant Extracts