title: The expression of B7-H1 and B7-H4 co-stimulatory molecules on myeloid and plasmacytoid dendritic cells in pre-eclampsia and normal pregnancy.
abstract: The aim of our study was to estimate the expression of B7-H1 and B7-H4 molecules on myeloid and plasmacytoid dendritic cells (DCs) in the peripheral blood of patients with pre-eclampsia, normal pregnant women and healthy non-pregnant women. Thirty-three patients with pre-eclampsia, 26 normal pregnant women, and 12 healthy non-pregnant women were included in the study. Dendritic cells were isolated from peripheral blood, stained with monoclonal antibodies against blood dendritic cell antigens and B7-H1 and B7-H4 molecules and estimated using flow cytometry. The expression of B7-H1 and B7-H4 molecules was significantly higher on CD1c(+) myeloid and CD303(+) plasmacytoid DCs in the first trimester of pregnancy than in the luteal phase of the ovarian cycle (CD1c(+)B7-H1(+): 19.19±10.55% vs. 11.99±6.79%; p<0.05; CD1c(+)B7-H4(+): 12.01±9.15% vs. 3.98±1.97%, p<0.001; CD303(+)B7-H1(+): 4.15±2.38% vs. 1.70±0.87%, p<0.05; CD303(+)B7-H4(+): 5.44±2.93% vs. 2.33±1.54%, p<0.01). Moreover, the expression of the B7-H1 molecule on CD1c(+) DCs in the second trimester of normal pregnancy was significantly higher than in the first trimester, but in the third trimester they decreased compared with the second trimester (II vs. I trimester: 32.23±11.30% vs. 19.19±10.55%, p<0.01; III vs. II trimester: 32.23±11.30% vs. 22.39±8.19%, p<0.01). The expression of B7-H1 molecule on CD1c(+) myeloid and CD303(+) plasmacytoid DCs was significantly lower in pre-eclampsia than in healthy third-trimester pregnant women (CD1c(+)B7-H1(+): 13.78±6.26% vs. 22.39±8.19%, p<0.05; CD303(+)B7-H1(+): 3.66±2.46% vs. 8.65±3.15%, p<0.01). Higher expressions of B7-H1 and B7-H4 molecules on CD1c(+) myeloid and CD303(+) plasmacytoid DCs in the first trimester of pregnancy suggest the role they play in the immunomodulation during early pregnancy.
authors.affiliation: Department of Obstetrics and Perinatology, Medical University of Lublin, Poland. Electronic address: dorotak@mp.pl.
authors.forename: Dorota
authors.initials: D
authors.lastname: Darmochwal-Kolarz
authors.forename: Magdalena
authors.initials: M
authors.lastname: Kludka-Sternik
authors.forename: Bogdan
authors.initials: B
authors.lastname: Kolarz
authors.forename: Tomasz
authors.initials: T
authors.lastname: Chmielewski
authors.forename: Jacek
authors.initials: J
authors.lastname: Tabarkiewicz
authors.forename: Jacek
authors.initials: J
authors.lastname: Rolinski
authors.forename: B
authors.initials: B
authors.lastname: Leszczynska-Gorzelak
authors.forename: Jan
authors.initials: J
authors.lastname: Oleszczuk
mesh_terms: D000328:Adult; D018949:Antigens, CD1 / Q000378:metabolism; D060890:B7-H1 Antigen / Q000378:metabolism*; D001773:Blood Cells / Q000276:immunology*; D002469:Cell Separation; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000276:immunology*; D005260:Female; D005434:Flow Cytometry; D006023:Glycoproteins / Q000378:metabolism; D006801:Humans; D037181:Lectins, C-Type / Q000378:metabolism; D008183:Luteal Phase / Q000276:immunology; D008562:Membrane Glycoproteins / Q000378:metabolism; D022423:Myeloid Cells / Q000276:immunology; D011225:Pre-Eclampsia / Q000276:immunology*; D011247:Pregnancy; D011264:Pregnancy Trimesters / Q000276:immunology; D011971:Receptors, Immunologic / Q000378:metabolism; D060907:V-Set Domain-Containing T-Cell Activation Inhibitor 1 / Q000378:metabolism*; D055815:Young Adult
chemical_list: D018949:Antigens, CD1; D060890:B7-H1 Antigen; C437944:CD1C protein, human; C443299:CLEC4C protein, human; D006023:Glycoproteins; D037181:Lectins, C-Type; D008562:Membrane Glycoproteins; D011971:Receptors, Immunologic; D060907:V-Set Domain-Containing T-Cell Activation Inhibitor 1; C475826:VTCN1 protein, human
keywords: B7-H1 molecule; B7-H4 molecule; Dendritic cells; Pre-eclampsia; Pregnancy