title: Effect of 950 MHz UHF electromagnetic radiation on biomarkers of oxidative damage, metabolism of UFA and antioxidants in the livers of young rats of different ages.
abstract: OBJECTIVE
  To assess the effect of 950 MHz ultra-high-frequency electromagnetic radiation (UHF EMR) on biomarkers of oxidative damage, as well as to verify the concentration of unsaturated fatty acids (UFA) and the expression of the catalase in the livers of rats of different ages.
  
  
  METHODS
  Twelve rats were equally divided into two groups as controls (CR) and exposed (ER), for each age (0, 6, 15 and 30 days). Radiation exposure lasted half an hour per day for up to 51 days (21 days of gestation and 6, 15 or 30 days of life outside the womb). The specific absorption rate (SAR) ranged from 1.3-1.0 W/kg. The damage to lipids, proteins and DNA was verified by thiobarbituric acid reactive substances (TBARS), protein carbonyls and comets, respectively. UFA were determined by gas chromatography with a flame ionization detector. The expression of catalase was by Western blotting.
  
  
  RESULTS
  The neonates had low levels of TBARS and concentrations of UFA after exposure. There was no age difference in the accumulation of protein carbonyls for any age. The DNA damage of ER 15 or 30 days was different. The exposed neonates exhibited lower expression of catalase.
  
  
  CONCLUSIONS
  950 MHz UHF EMR does not cause oxidative stress (OS), and it is not genotoxic to the livers of neonates or those of 6 and 15 day old rats, but it changes the concentrations of polyunsaturated fatty acid (PUFA) in neonates. For rats of 30 days, no OS, but it is genotoxic to the livers of ER to total body irradiation.
authors.affiliation: Biotechnology Center.
authors.forename: Orlando V
authors.initials: OV
authors.lastname: Furtado-Filho
authors.forename: Juliana B
authors.initials: JB
authors.lastname: Borba
authors.forename: Alexsandro
authors.initials: A
authors.lastname: Dallegrave
authors.forename: Tânia M
authors.initials: TM
authors.lastname: Pizzolato
authors.forename: João A P
authors.initials: JA
authors.lastname: Henriques
authors.forename: José C F
authors.initials: JC
authors.lastname: Moreira
authors.forename: Jenifer
authors.initials: J
authors.lastname: Saffi
mesh_terms: D000375:Aging; D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D015415:Biomarkers; D002374:Catalase / Q000378:metabolism; D004249:DNA Damage; D004574:Electromagnetic Fields; D005231:Fatty Acids, Unsaturated / Q000378:metabolism*; D005260:Female; D015227:Lipid Peroxidation / Q000528:radiation effects; D008099:Liver / Q000378:metabolism / Q000528:radiation effects*; D008297:Male; D018384:Oxidative Stress / Q000528:radiation effects*; D011829:Radiation Dosage; D051381:Rats
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D005231:Fatty Acids, Unsaturated; D002374:Catalase