title: Antifungal activities of diphenyl diselenide alone and in combination with fluconazole or amphotericin B against Candida glabrata.
abstract: Here, we evaluated combinations of diphenyl diselenide [(PhSe)2] with fluconazole and amphotericin B in a checkerboard assay against clinical Candida glabrata strains. Minimal inhibitory concentration (geometric mean) ranged from 0.25 to >64 (5.16 μg/mL) for (PhSe)2, 1 to 32 (5.04 μg/mL) for fluconazole and 0.06 to 0.5 (0.18 μg/mL) for amphotericin B. Synergistic (76.66 %) and indifferent (23.34 %) interactions were observed for (PhSe)2 + amphotericin B combination. (PhSe)2 + fluconazole combination demonstrated indifferent (50 %) and antagonistic (40 %) interactions, whereas synergistic interactions were observed in 10 % of the isolates. New experimental in vivo protocols are necessary and will promote a better understanding of the antimicrobial activity of (PhSe)2 against C. glabrata and its use as an adjuvant therapy with antifungal agents.
authors.affiliation: Programa de Pós-Graduação em Ciências Farmacêuticas, Centro de Ciências da Saúde, Universidade Federal de Santa Maria (UFSM), Campus UFSM, Prédio 20, sala 4139, Santa Maria, RS, 97105-900, Brazil. laura-denardi@hotmail.com
authors.forename: Laura Bedin
authors.initials: LB
authors.lastname: Denardi
authors.forename: Débora Alves Nunes
authors.initials: DA
authors.lastname: Mario
authors.forename: Erico Silva
authors.initials: ES
authors.lastname: de Loreto
authors.forename: Cristina Wayne
authors.initials: CW
authors.lastname: Nogueira
authors.forename: Janio Morais
authors.initials: JM
authors.lastname: Santurio
authors.forename: Sydney Hartz
authors.initials: SH
authors.lastname: Alves
mesh_terms: D000666:Amphotericin B / Q000494:pharmacology*; D000935:Antifungal Agents / Q000494:pharmacology*; D001555:Benzene Derivatives / Q000494:pharmacology*; D041221:Candida glabrata / Q000187:drug effects*; D004347:Drug Interactions; D015725:Fluconazole / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D016566:Organoselenium Compounds / Q000494:pharmacology*
chemical_list: D000935:Antifungal Agents; D001555:Benzene Derivatives; D016566:Organoselenium Compounds; C061132:diphenyldiselenide; D000666:Amphotericin B; D015725:Fluconazole