title: Metabolomics Study of Resina Draconis on Myocardial Ischemia Rats Using Ultraperformance Liquid Chromatography/Quadrupole Time-of-Flight Mass Spectrometry Combined with Pattern Recognition Methods and Metabolic Pathway Analysis.
abstract: Resina draconis (bright red resin isolated from Dracaena cochinchinensis, RD) has been clinically used for treatment of myocardial ischemia (MI) for many years. However, the mechanisms of its pharmacological action on MI are still poorly understood. This study aimed to characterize the plasma metabolic profiles of MI and investigate the mechanisms of RD on MI using ultraperformance liquid chromatography/quadrupole time-of-flight mass spectrometry-based metabolomics combined with pattern recognition methods and metabolic pathway analysis. Twenty metabolite markers characterizing metabolic profile of MI were revealed, which were mainly involved in aminoacyl-tRNA biosynthesis, phenylalanine, tyrosine, and tryptophan biosynthesis, vascular smooth muscle contraction, sphingolipid metabolism, and so forth. After RD treatment, however, levels of seven MI metabolite markers, including phytosphingosine, sphinganine, acetylcarnitine, cGMP, cAMP, L-tyrosine, and L-valine, were turned over, indicating that RD is likely to alleviate MI through regulating the disturbed vascular smooth muscle contraction, sphingolipid metabolism, phenylalanine metabolism, and BCAA metabolism. To our best knowledge, this is the first comprehensive study to investigate the mechanisms of RD for treating MI, from a metabolomics point of view. Our findings are very valuable to gain a better understanding of MI metabolic profiles and provide novel insights for exploring the mechanisms of RD on MI.
authors.affiliation: Department of Pharmaceutical Analysis, School of Pharmacy, Second Military Medical University, 325 Guohe Road, Shanghai 200433, China ; Shanghai Key Laboratory for Pharmaceutical Metabolite Research, 325 Guohe Road, Shanghai 200433, China ; Shanghai Research Centre for Drug (Chinese Materia Medica) Metabolism, 325 Guohe Road, Shanghai 200433, China.
authors.forename: Yunpeng
authors.initials: Y
authors.lastname: Qi
authors.forename: Haiwei
authors.initials: H
authors.lastname: Gu
authors.forename: Yunlong
authors.initials: Y
authors.lastname: Song
authors.forename: Xin
authors.initials: X
authors.lastname: Dong
authors.forename: Aijun
authors.initials: A
authors.lastname: Liu
authors.forename: Ziyang
authors.initials: Z
authors.lastname: Lou
authors.forename: Guorong
authors.initials: G
authors.lastname: Fan
authors.forename: Yifeng
authors.initials: Y
authors.lastname: Chai