title: Highly multiplexed antibody suspension bead arrays for plasma protein profiling.
abstract: Alongside the increasing availability of affinity reagents, antibody microarrays have become a powerful tool to screen for target proteins in complex samples. Applying directly labeled samples onto arrays instead of using sandwich assays offers an approach to facilitate a systematic, high-throughput, and flexible exploration of protein profiles in body fluids such as serum or plasma. As an alternative to planar arrays, a system based on color-coded beads for the creation of antibody arrays in suspension has become available to offer a microtiter plate-based option for screening larger number of samples with variable sets of capture reagents. A procedure was established for analyzing biotinylated samples without the necessity to remove excess labeling substance. We have shown that this assay system allows detecting proteins down into lower pico-molar and higher pg/ml levels with dynamic ranges over three orders of magnitude. Presently, this workflow enables the profiling of 384 samples for up to 384 proteins per assay.
authors.affiliation: Science for Life Laboratory Stockholm, School of Biotechnology, KTH-Royal Institute of Technology, Stockholm, Sweden.
authors.forename: Kimi
authors.initials: K
authors.lastname: Drobin
authors.forename: Peter
authors.initials: P
authors.lastname: Nilsson
authors.forename: Jochen M
authors.initials: JM
authors.lastname: Schwenk
mesh_terms: D055910:Antibodies, Immobilized / Q000737:chemistry*; D019921:Biotinylation; D001798:Blood Proteins / Q000032:analysis*; D006801:Humans; D040081:Protein Array Analysis / Q000379:methods*
chemical_list: D055910:Antibodies, Immobilized; D001798:Blood Proteins