title: Contamination of patient blood samples by avian RBCs from control material during automated hematology analysis.
abstract: OBJECTIVE
  Atypical nucleated RBCs (NRBCs) found on several patient blood smears between 2010 and 2012 were noted to resemble avian RBCs. NRBCs are not normally found in the circulation beyond the neonatal period and may indicate hematologic disease, malignancy in the bone marrow, or other severe conditions. Our blood smears with unusual NRBCs did not contain other abnormalities that typically accompany NRBCs, such as immature cells or dysplastic granulocytes. To investigate this anomaly, we considered possibilities such as contaminated collection tubes and instrument problems. The Retic-C Cell Control used with the LH 750 Hematology Analyzer contains a mixture of human and avian RBCs.
  
  
  METHODS
  CBC count with differential tests were performed on blanks and routine laboratory samples run immediately after the Retic-C Cell Control on the LH 750 and LH 780 analyzers to recreate the conditions that might cause spillage into the next tube.
  
  
  RESULTS
  We experimentally reproduced the phenomenon of contamination of a subsequent tube with avian cells from a multiply punctured reticulocyte control tube.
  
  
  CONCLUSIONS
  We concluded that the NRBCs likely represented avian RBCs from the Retic-C Cell Control that had been introduced into the patient tubes.
authors.affiliation: Stony Brook University Medical Center, UH, Level 3, Rm 532, Stony Brook, NY 11794-7300, USA. Lisa.senzel@sbumed.org
authors.forename: Lisa
authors.initials: L
authors.lastname: Senzel
authors.forename: Bruce
authors.initials: B
authors.lastname: Kube
authors.forename: Mabel
authors.initials: M
authors.lastname: Lou
authors.forename: Alice
authors.initials: A
authors.lastname: Gibbs
authors.forename: Tahmeena
authors.initials: T
authors.lastname: Ahmed
authors.forename: Brent
authors.initials: B
authors.lastname: Hall
mesh_terms: D000818:Animals; D057205:Automation, Laboratory; D001717:Birds / Q000097:blood*; D001800:Blood Specimen Collection; D004900:Erythroblasts / Q000166:cytology; D004906:Erythrocyte Count / Q000295:instrumentation*; D004912:Erythrocytes / Q000166:cytology*; D006402:Hematologic Diseases / Q000175:diagnosis*; D006801:Humans; D015203:Reproducibility of Results; D012156:Reticulocytes / Q000166:cytology
keywords: Automated hematology analyzer; Avian RBCs; Contamination; Nucleated RBCs