title: A novel glycoprotein from mushroom Hypsizygus marmoreus (Peck) Bigelow with growth inhibitory effect against human leukemic U937 cells.
abstract: This study was to isolate the anti-leukaemic component from edible mushroom Hypsizygus marmoreus (Peck) Bigelow. Crude protein was extracted from the basidioma, and then purified with DEAE-Sepharose CL-6B ion exchange chromatography followed by Sephacryl S-300 gel filtration. A protein which exerted high growth inhibitory effect on human leukaemic U937 cells and sufficient toxicological safety on normal human white blood cells was isolated and named HM-3A. Electrophoresis showed HM-3A approximately 52kDa in size. N-terminal analysis found the amino acid sequence ATTQWKTSAA and confirmed HM-3A a novel protein. High-performance anion-exchange column chromatography revealed HM-3A a glycoprotein with galactose as the major monosaccharide. Haemagglutination assay proved it non-lectin. We suggest that HM-3A is worth further investigation for antitumour use.
authors.affiliation: Graduate Institute of Food Science and Technology, National Taiwan University, Taipei 106, Taiwan.
authors.forename: Pei-Feng
authors.initials: PF
authors.lastname: Tsai
authors.forename: Chia-Yu
authors.initials: CY
authors.lastname: Ma
authors.forename: James Swi-Bea
authors.initials: JS
authors.lastname: Wu
mesh_terms: D000363:Agaricales / Q000737:chemistry*; D000595:Amino Acid Sequence; D049109:Cell Proliferation / Q000187:drug effects*; D005656:Fungal Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D006023:Glycoproteins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D006131:Growth Inhibitors / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D007938:Leukemia / Q000503:physiopathology*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D020298:U937 Cells
chemical_list: D005656:Fungal Proteins; D006023:Glycoproteins; D006131:Growth Inhibitors