title: Deep sequencing revealed genome-wide single-nucleotide polymorphism and plasmid content of Erwinia amylovora strains isolated in Middle Atlas, Morocco.
abstract: Erwinia amylovora causes economic losses that affect pear and apple production in Morocco. Here, we report comparative genomics of four Moroccan E. amylovora strains with the European strain CFBP1430 and North-American strain ATCC49946. Analysis of single nucleotide polymorphisms (SNPs) revealed genetic homogeneity of Moroccan's strains and their proximity to the European strain CFBP1430. Moreover, the collected sequences allowed the assembly of a 65 kpb plasmid, which is highly similar to the plasmid pEI70 harbored by several European E. amylovora isolates. This plasmid was found in 33% of the 40 E. amylovora strains collected from several host plants in 2009 and 2010 in Morocco.
authors.affiliation: Université Moulay Ismail, Faculté des Sciences, Département de Biologie, Meknès, Morocco; Institut des Sciences du Végétal, CNRS, Avenue de la Terrasse, 91 198 Gif-sur-Yvette cedex, France.
authors.forename: Najat
authors.initials: N
authors.lastname: Hannou
authors.forename: Samuel
authors.initials: S
authors.lastname: Mondy
authors.forename: Sara
authors.initials: S
authors.lastname: Planamente
authors.forename: Mohieddine
authors.initials: M
authors.lastname: Moumni
authors.forename: Pablo
authors.initials: P
authors.lastname: Llop
authors.forename: María
authors.initials: M
authors.lastname: López
authors.forename: Charles
authors.initials: C
authors.lastname: Manceau
authors.forename: Marie-Anne
authors.initials: MA
authors.lastname: Barny
authors.forename: Denis
authors.initials: D
authors.lastname: Faure
mesh_terms: D004783:Environmental Microbiology; D044084:Erwinia amylovora / Q000235:genetics* / Q000302:isolation & purification; D005060:Europe; D016680:Genome, Bacterial; D059014:High-Throughput Nucleotide Sequencing; D027845:Malus / Q000382:microbiology; D008969:Molecular Sequence Data; D009018:Morocco; D009656:North America; D010957:Plasmids; D020641:Polymorphism, Single Nucleotide; D031989:Pyrus / Q000382:microbiology
keywords: Erwinia amylovora; Fire blight; Genome; Plasmid; SNP; Virulence