title: Goniothalamin selectively induces apoptosis on human hepatoblastoma cells through caspase-3 activation.
abstract: Goniothalamin is a biologically active styrylpyrone derivative isolated from various Goniothalamus species. The ability of goniothalamin to induce apoptosis via caspase-3 activation against hepatoblastoma (HepG2) and normal liver cells (Chang cells) was studied using morphological and biochemical evaluations. HepG2 and Chang cells were treated with goniothalamin for 72 h and analysed by TUNEL and Annexin-V/PI staining. Furthermore, the post-mitochondrial caspase-3 was quantified using ELISA. In view of our results, goniothalamin induced apoptosis on treated cells via alteration of cellular membrane integrity and cleavage of DNA. On the other hand, post-mitochondrial caspase-3 activity was significantly elevated in HepG2 cells treated with goniothalamin after 72 h. These findings suggest that goniothalamin induced apoptosis on HepG2 liver cancer cells via induction of caspase-3 with less sensitivity on the cell line of Chang cells.
authors.affiliation: a Department of Cell and Molecular Biology , Faculty of Biotechnology and Biomolecular Sciences, University Putra Malaysia (UPM) , 43400 Serdang, Selangor , Malaysia.
authors.forename: Mothanna
authors.initials: M
authors.lastname: Al-Qubaisi
authors.forename: Rozita
authors.initials: R
authors.lastname: Rosli
authors.forename: Tamilselvan
authors.initials: T
authors.lastname: Subramani
authors.forename: Abdul Rahman
authors.initials: AR
authors.lastname: Omar
authors.forename: Swee Keong
authors.initials: SK
authors.lastname: Yeap
authors.forename: Abdul Manaf
authors.initials: AM
authors.lastname: Ali
authors.forename: Noorjahan Banu
authors.initials: NB
authors.lastname: Alitheen
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3 / Q000378:metabolism*; D002478:Cells, Cultured; D004789:Enzyme Activation; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry; D056945:Hep G2 Cells; D018197:Hepatoblastoma / Q000201:enzymology / Q000473:pathology*; D006801:Humans; D020287:In Situ Nick-End Labeling; D008113:Liver Neoplasms / Q000201:enzymology / Q000473:pathology*; D011753:Pyrones / Q000494:pharmacology*
chemical_list: D011753:Pyrones; C103280:goniothalamin; D053148:Caspase 3