title: Distribution of nine virulence-associated genes in Campylobacter jejuni and C. coli isolated from broiler feces in Shiraz, Southern Iran.
abstract: To investigate the prevalence of nine virulence and toxin genes of Campylobacter, a total of 90 Campylobacter strains including 48 C. jejuni and 42 C. coli were recovered from chicken feces by cultivation methods. The isolates were identified on the basis of polymerase chain reaction (PCR) detection of 16SrRNA and multiplex PCR for determining two species. For confirmed strains, PCR was carried out for the presence of virulence genes using specific primers. Data were analyzed by SPSS software, version 12.0.1. The cadF gene and three genes associated with cytolethal distending toxin were present in 100% of isolates. Plasmid virB11 gene was not found in any of the Campylobacter isolates, and the prevalence of pldA, wlaN, iamA, and cgtB genes were 92.22%, 82.22%, 81.11%, and 22.22%, respectively. The findings revealed that the distribution of the majority of these genes were not dissimilar among Campylobacter species. The results emphasized that many of the pathogenic C. jejuni and C. coli may have these genes, and the Campylobacter strains with poultry origin have pathogenic potential properties for humans.
authors.affiliation: Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran.
authors.forename: Rahem
authors.initials: R
authors.lastname: Khoshbakht
authors.forename: Mohammad
authors.initials: M
authors.lastname: Tabatabaei
authors.forename: Saeid
authors.initials: S
authors.lastname: Hosseinzadeh
authors.forename: Seied Shahram
authors.initials: SS
authors.lastname: Shekarforoush
authors.forename: Hesamaddin Shirzad
authors.initials: HS
authors.lastname: Aski
mesh_terms: D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics; D001427:Bacterial Toxins / Q000235:genetics; D017000:Campylobacter coli / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity; D016123:Campylobacter jejuni / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity; D002352:Carrier Proteins / Q000235:genetics; D017931:DNA Primers; D004269:DNA, Bacterial / Q000235:genetics; D005243:Feces / Q000382:microbiology*; D005506:Food Contamination / Q000032:analysis; D005516:Food Microbiology; D005798:Genes, Bacterial; D007492:Iran; D008460:Meat / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D011200:Poultry; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D014774:Virulence / Q000235:genetics
chemical_list: D001425:Bacterial Outer Membrane Proteins; D001427:Bacterial Toxins; C107872:CadF protein, Campylobacter jejuni; D002352:Carrier Proteins; D017931:DNA Primers; D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S; C067044:cytolethal distending toxin