title: Comparison of selected biomarkers in flounder (Platichthys flesus L.) from the Douro (Portugal) and Vistula (Poland) River estuaries.
abstract: Sixty female flounder (Platichthys flesus) were collected in Autumn 2011, 15 from each of the following sampling sites: at the mouths of the Douro and Vistula Rivers, and at nearby open sea locations. The aim of the study was to assess several biomarkers in the two geographically distant regions. Hepatic EROD, GST, SOD, GPx, POx, LP; muscular AChE, BChE, LP; and branchial Na(+)/K(+)-ATPase were analysed. Moreover, BTI, PY, and three gross morphometric indices were calculated. The results were analysed with t-test, ANOVA, and PCA. Many differences were found between the open sea sites and the river mouths, mainly in Portugal, and between the two rivers. Salinity and pollution seem to be the main factors that affected the biomarkers. Effects of chronic pollution were observed at the river mouths, and an indication of a possible temporary exposure to pollutants was found at the open ocean site in Portugal.
authors.affiliation: CIIMAR - Centre for Marine Environmental Research, Rua dos Bragas, 289, 4050-123 Porto, Portugal. justyna.kopecka@yahoo.com
authors.forename: Justyna
authors.initials: J
authors.lastname: Kopecka-Pilarczyk
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D019363:Cytochrome P-450 CYP1A1 / Q000378:metabolism; D004784:Environmental Monitoring / Q000379:methods*; D063366:Estuaries / Q000706:statistics & numerical data*; D005260:Female; D005432:Flounder / Q000378:metabolism*; D005979:Glutathione Peroxidase / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D015227:Lipid Peroxidation; D011044:Poland; D011174:Portugal; D045483:Rivers / Q000737:chemistry; D013482:Superoxide Dismutase / Q000378:metabolism; D014874:Water Pollutants, Chemical / Q000032:analysis / Q000378:metabolism*; D014877:Water Pollution, Chemical / Q000706:statistics & numerical data
chemical_list: D015415:Biomarkers; D014874:Water Pollutants, Chemical; D002374:Catalase; D005979:Glutathione Peroxidase; D019363:Cytochrome P-450 CYP1A1; D013482:Superoxide Dismutase; D005982:Glutathione Transferase; D000110:Acetylcholinesterase
keywords: Biomarkers; Douro River; Flounder; Pollution; Salinity; Vistula River