title: Physico-functional and mechanical properties of chitosan and calcium salts incorporated fish gelatin scaffolds.
abstract: Four types of fish gelatin scaffolds viz. gelatin (G), gelatin-chitosan (GC), gelatin-calcium acetate (GCA) and gelatin-chitosan-calcium acetate (GCCA) prepared were investigated for their functional properties, biomechanical strength, microstructural changes in relation to biodegradation. GC scaffold was superior with pH 3.15 and viscosity 9.40 cP. Chitosan and calcium acetate improved tensile strength (TS) and Young's modulus (YM), but lowered elongation at break (EAB). GCCA scaffold possessed moderate TS of 19.6 MPa, EAB of 4.76% and YM of 185 MPa. Foaming ability ratio of GC scaffold was high (3.41). GCA and GCCA scaffolds remained for 4 days before complete in vitro biodegradation. GC scaffold had larger cavities (180-300 μm) that were responsible for low swelling ratios and shrinkage factor. GCCA scaffold with moderate swelling rates, mechanical, functional properties and lowered biodegradation rate were found more suitable for biomedical applications.
authors.affiliation: Department of Fish Quality Assurance and Management, Fisheries College and Research Institute, Tamil Nadu Fisheries University, Tuticorin 628 008, India.
authors.forename: E
authors.initials: E
authors.lastname: Jeevithan
authors.forename: R
authors.initials: R
authors.lastname: Jeya Shakila
authors.forename: A
authors.initials: A
authors.lastname: Varatharajakumar
authors.forename: G
authors.initials: G
authors.lastname: Jeyasekaran
authors.forename: D
authors.initials: D
authors.lastname: Sukumar
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D002118:Calcium / Q000737:chemistry*; D048271:Chitosan / Q000737:chemistry*; D005399:Fishes / Q000235:genetics*; D005780:Gelatin / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D055595:Mechanical Phenomena; D012492:Salts / Q000737:chemistry*; D054457:Tissue Scaffolds / Q000737:chemistry*; D014783:Viscosity
chemical_list: D001672:Biocompatible Materials; D012492:Salts; D005780:Gelatin; D048271:Chitosan; D002118:Calcium
keywords: Bio-degradation; Fish gelatin; Mechanical properties; Microstructure; Scaffold