title: Genotype analysis based on intergenic spacer 1 sequences of Trichosporon asahii collected in Taiwan.
abstract: Among 32 Trichosporon asahii isolates collected in four rounds of the Taiwan Surveillance of Antimicrobial Resistance of Yeasts (TSARY) studies, conducted in 1999, 2002, 2006, and 2010, five different intergenic spacer 1 (IGS1) genotypes were detected. Genotype 1 was the most common (43.8%), followed by genotypes 3 (28.1%), 7 (12.5%), 5 (9.4%), and 4 (6.3%). Interestingly, genotype 7 was more prevalent in Taiwan than in other areas (P = 0.01); while we did not find a significant association between IGS1 genotype and susceptibility to antifungal drugs, we did note that the majority of isolates of T. asahii were susceptible to both fluconazole and voriconazole, consistent with previous reports. A higher proportion of isolates (P = 0.05) collected in 2010 (4/12, 33.3%) had high amphotericin B MICs (≥ 2 mg/l) than those collected in the previous three TSARYs (1/21, 5%). Hence, the new data of genotypes and drug susceptibilities in the present study may contribute to the epidemiology of T. asahii.
authors.affiliation: * Department of Biological Science and Technology.
authors.forename: Yun-Liang
authors.initials: YL
authors.lastname: Yang
authors.forename: Yi-Wen
authors.initials: YW
authors.lastname: Liu
authors.forename: Hui-Ting
authors.initials: HT
authors.lastname: Chen
authors.forename: Moan-Shane
authors.initials: MS
authors.lastname: Tsai
authors.forename: Wen-Li
authors.initials: WL
authors.lastname: Chu
authors.forename: Hsiu-Jung
authors.initials: HJ
authors.lastname: Lo
mesh_terms: D000935:Antifungal Agents / Q000494:pharmacology; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics*; D021901:DNA, Intergenic / Q000737:chemistry / Q000235:genetics*; D025141:Drug Resistance, Fungal; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D017720:Molecular Epidemiology; D015995:Prevalence; D017422:Sequence Analysis, DNA; D013624:Taiwan; D014250:Trichosporon / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D060586:Trichosporonosis / Q000453:epidemiology / Q000382:microbiology
chemical_list: D000935:Antifungal Agents; D004271:DNA, Fungal; D021901:DNA, Intergenic