title: Induced pluripotent stem cells derived from rabbits exhibit some characteristics of naïve pluripotency.
abstract: Not much is known about the molecular and functional features of pluripotent stem cells (PSCs) in rabbits. To address this, we derived and characterized 2 types of rabbit PSCs from the same breed of New Zealand White rabbits: 4 lines of embryonic stem cells (rbESCs), and 3 lines of induced PSCs (rbiPSCs) that were obtained by reprogramming adult skin fibroblasts. All cell lines required fibroblast growth factor 2 for their growth and proliferation. All rbESC lines showed molecular and functional properties typically associated with primed pluripotency. The cell cycle of rbESCs had a prolonged G1 phase and a DNA damage checkpoint before entry into the S phase, which are the 2 features typically associated with the somatic cell cycle. In contrast, the rbiPSC lines exhibited some characteristics of naïve pluripotency, including resistance to single-cell dissociation by trypsin, robust activity of the distal enhancer of the mouse Oct4 gene, and expression of naïve pluripotency-specific genes, as defined in rodents. According to gene expression profiles, rbiPSCs were closer to the rabbit inner cell mass (ICM) than rbESCs. Furthermore, rbiPSCs were capable of colonizing the ICM after aggregation with morulas. Therefore, we propose that rbiPSCs self-renew in an intermediate state between naïve and primed pluripotency, which represents a key step toward the generation of bona fide naïve PSC lines in rabbits.
authors.affiliation: INSERM, U846, Stem Cell and Brain Institute , 18 Avenue du Doyen Jean Lépine, F-69500 Bron , France ; Stem Cell and Brain Institute , F-69500 Bron , France ; Université de Lyon , F-69100 Villeurbanne , France ; INRA, USC1361, F-69500 Bron , France.
authors.forename: Pierre
authors.initials: P
authors.lastname: Osteil
authors.forename: Yann
authors.initials: Y
authors.lastname: Tapponnier
authors.forename: Suzy
authors.initials: S
authors.lastname: Markossian
authors.forename: Murielle
authors.initials: M
authors.lastname: Godet
authors.forename: Barbara
authors.initials: B
authors.lastname: Schmaltz-Panneau
authors.forename: Luc
authors.initials: L
authors.lastname: Jouneau
authors.forename: Cédric
authors.initials: C
authors.lastname: Cabau
authors.forename: Thierry
authors.initials: T
authors.lastname: Joly
authors.forename: Thierry
authors.initials: T
authors.lastname: Blachère
authors.forename: Elen
authors.initials: E
authors.lastname: Gócza
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Bernat
authors.forename: Martine
authors.initials: M
authors.lastname: Yerle
authors.forename: Hervé
authors.initials: H
authors.lastname: Acloque
authors.forename: Sullivan
authors.initials: S
authors.lastname: Hidot
authors.forename: Zsuzsanna
authors.initials: Z
authors.lastname: Bosze
authors.forename: Véronique
authors.initials: V
authors.lastname: Duranthon
authors.forename: Pierre
authors.initials: P
authors.lastname: Savatier
authors.forename: Marielle
authors.initials: M
authors.lastname: Afanassieff
keywords: Cell cycle; Embryonic stem cell; Induced pluripotent stem cell; Pluripotency; Rabbit model