title: Natural occurrence of White spot syndrome virus and Infectious hypodermal and hematopoietic necrosis virus in Neohelice granulata crab.
abstract: White spot syndrome virus (WSSV) and Infectious hypodermal and hematopoietic necrosis virus (IHHNV) are two infectious agents associated to economic losses in shrimp aquaculture. As virus spread occurs through vectors and hosts, this study sought to verify the presence of WSSV and IHHNV in Neohelice granulata crab from Lagoa dos Patos estuary in Brazil and nearby shrimp farms. DNA extractions were performed with phenol/chloroform protocol. Molecular diagnosis was carried out by nested PCR for WSSV and one-step PCR for IHHNV. Results showed the presence of WSSV on crabs of both Lagoa dos Patos and farms, while IHHNV was found only on crabs collected in estuary. This is the first study to report IHHNV presence in N. granulata. Moreover, as analyzed crabs had no clinical symptoms or showed in situ mortality, we suggest its use as a bioindicator for virus occurrence in aquatic environments.
authors.affiliation: Instituto de Pesquisas Veterinárias Desidério Finamor, Fundação Estadual de Pesquisa Agropecuária FEPAGRO - Saúde Animal, Eldorado do Sul, RS 92990000, Brazil. liscavalli@gmail.com
authors.forename: Lissandra Souto
authors.initials: LS
authors.lastname: Cavalli
authors.forename: Carolina Reyes
authors.initials: CR
authors.lastname: Batista
authors.forename: Bruna F S
authors.initials: BF
authors.lastname: Nornberg
authors.forename: Fabiana Quoos
authors.initials: FQ
authors.lastname: Mayer
authors.forename: Fabiana K
authors.initials: FK
authors.lastname: Seixas
authors.forename: Luis Alberto
authors.initials: LA
authors.lastname: Romano
authors.forename: Luis Fernando
authors.initials: LF
authors.lastname: Marins
authors.forename: Paulo César
authors.initials: PC
authors.lastname: Abreu
mesh_terms: D000818:Animals; D017756:Aquaculture; D003386:Brachyura / Q000821:virology*; D001938:Brazil; D019223:Densovirinae / Q000235:genetics / Q000302:isolation & purification*; D004784:Environmental Monitoring / Q000379:methods; D016133:Polymerase Chain Reaction; D046848:White spot syndrome virus 1 / Q000235:genetics / Q000302:isolation & purification*
keywords: Bioindicator; IHHNV; Neohelice granulata; Viral disease; WSSV