title: Decreased plasma ADAMTS-13 activity as a predictor of postoperative bleeding in cyanotic congenital heart disease.
abstract: OBJECTIVE
  To analyze the preoperative plasma antigenic concentration and activity of von Willebrand factor and its main cleaving protease ADAMTS-13 in pediatric patients with cyanotic congenital heart disease undergoing surgical treatment and investigate possible correlations with postoperative bleeding.
  
  
  METHODS
  Plasma antigenic concentrations (von Willebrand factor:Ag and ADAMTS-13:Ag) were measured using enzyme-linked immunoassays. Collagen-binding assays were developed to measure biological activities (von Willebrand factor:collagen binding and ADAMTS-13 activity). The multimeric structure of von Willebrand factor was analyzed using Western immunoblotting. Demographic, diagnostic, and general and specific laboratory data and surgery-related variables were subjected to univariate, bivariate, and multivariate analysis for the prediction of postoperative bleeding.
  
  
  RESULTS
  Forty-eight patients were enrolled, with ages ranging from 9 months to 7.6 years (median 2.5 years). The plasma concentrations of von Willebrand factor:Ag and ADAMTS-13:Ag were decreased by 65 and 82%, respectively, in the patients compared with the controls (p<0.001). An increased density of low-molecular-weight fractions of von Willebrand factor, which are suggestive of proteolytic degradation (p = 0.0081), was associated with decreased ADAMTS-13 activity, which was likely due to ADAMTS-13 consumption (71% of controls, p = 0.0029) and decreased von Willebrand factor:collagen binding (76% of controls, p = 0.0004). Significant postoperative bleeding occurred in 13 patients. The preoperative ADAMTS-13 activity of <64.6% (mean level for the group), preoperative activated partial thromboplastin time, and the need for cardiopulmonary bypass were characterized as independent risk factors for postoperative bleeding, with respective hazard ratios of 22.35 (95% CI 1.69 to 294.79), 1.096 (95% CI 1.016 to 1.183), and 37.43 (95% CI 1.79 to 782.73).
  
  
  CONCLUSIONS
  Low plasma ADAMTS-13 activity is a risk factor for postoperative bleeding in children with cyanotic congenital heart disease, particularly in children undergoing cardiopulmonary bypass.
authors.affiliation: Fundação Pró-Sangue Hemocentro de São Paulo, São Paulo/SP, Brazil. aablopes@usp.br
authors.forename: Rosangela P S
authors.initials: RP
authors.lastname: Soares
authors.forename: Sérgio P
authors.initials: SP
authors.lastname: Bydlowski
authors.forename: Marcelo B
authors.initials: MB
authors.lastname: Jatene
authors.forename: Janete Ferreira
authors.initials: JF
authors.lastname: Hironaka
authors.forename: Antonio Augusto
authors.initials: AA
authors.lastname: Lopes
mesh_terms: D051722:ADAM Proteins / Q000097:blood* / Q000502:physiology; D000071120:ADAMTS13 Protein; D000704:Analysis of Variance; D001777:Blood Coagulation / Q000502:physiology; D015153:Blotting, Western; D002648:Child; D002675:Child, Preschool; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006330:Heart Defects, Congenital / Q000097:blood* / Q000601:surgery; D006801:Humans; D007223:Infant; D008297:Male; D019106:Postoperative Hemorrhage / Q000097:blood* / Q000209:etiology; D011237:Predictive Value of Tests; D012016:Reference Values; D012307:Risk Factors; D014841:von Willebrand Factor / Q000032:analysis* / Q000502:physiology
chemical_list: D014841:von Willebrand Factor; D051722:ADAM Proteins; D000071120:ADAMTS13 Protein; C099604:ADAMTS13 protein, human