title: The early activation marker CD69 regulates the expression of chemokines and CD4 T cell accumulation in intestine.
abstract: Migration of naïve and activated lymphocytes is regulated by the expression of various molecules such as chemokine receptors and ligands. CD69, the early activation marker of C-type lectin domain family, is also shown to regulate the lymphocyte migration by affecting their egress from the thymus and secondary lymphoid organs. Here, we aimed to investigate the role of CD69 in accumulation of CD4 T cells in intestine using murine models of inflammatory bowel disease. We found that genetic deletion of CD69 in mice increases the expression of the chemokines CCL-1, CXCL-10 and CCL-19 in CD4(+) T cells and/or CD4(-) cells. Efficient in vitro migration of CD69-deficient CD4 T cells toward the chemokine stimuli was the result of increased expression and/or affinity of chemokine receptors. In vivo CD69(-/-) CD4 T cells accumulate in the intestine in higher numbers than B6 CD4 T cells as observed in competitive homing assay, dextran sodium sulphate (DSS)-induced colitis and antigen-specific transfer colitis. In DSS colitis CD69(-/-) CD4 T cell accumulation in colonic lamina propria (cLP) was associated with increased expression of CCL-1, CXCL-10 and CCL-19 genes. Furthermore, treatment of DSS-administrated CD69(-/-) mice with the mixture of CCL-1, CXCL-10 and CCL-19 neutralizing Abs significantly decreased the histopathological signs of colitis. Transfer of OT-II×CD69(-/-) CD45RB(high) CD4 T cells into RAG(-/-) hosts induced CD4 T cell accumulation in cLP. This study showed CD69 as negative regulator of inflammatory responses in intestine as it decreases the expression of chemotactic receptors and ligands and reduces the accumulation of CD4 T cells in cLP during colitis.
authors.affiliation: Department of Internal Medicine I, University of Ulm, Ulm, Germany. katarina.radulovic@uni-ulm.de
authors.forename: Katarina
authors.initials: K
authors.lastname: Radulovic
authors.forename: Valerio
authors.initials: V
authors.lastname: Rossini
authors.forename: Calin
authors.initials: C
authors.lastname: Manta
authors.forename: Karlheinz
authors.initials: K
authors.lastname: Holzmann
authors.forename: Hans A
authors.initials: HA
authors.lastname: Kestler
authors.forename: Jan Hendrik
authors.initials: JH
authors.lastname: Niess
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000235:genetics / Q000378:metabolism*; D000945:Antigens, Differentiation, T-Lymphocyte / Q000235:genetics / Q000378:metabolism*; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D054402:Chemokine CCL1; D054415:Chemokine CCL19; D054357:Chemokine CXCL10; D018925:Chemokines / Q000276:immunology*; D003093:Colitis, Ulcerative / Q000139:chemically induced / Q000276:immunology* / Q000378:metabolism; D016264:Dextran Sulfate / Q000633:toxicity; D004797:Enzyme-Linked Immunosorbent Assay; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000276:immunology*; D037181:Lectins, C-Type / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D046228:Microarray Analysis; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D018709:Statistics, Nonparametric; D000099192:CD69 Antigens
chemical_list: D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D054402:Chemokine CCL1; D054415:Chemokine CCL19; D054357:Chemokine CXCL10; D018925:Chemokines; D016264:Dextran Sulfate; D037181:Lectins, C-Type; D000099192:CD69 Antigens