title: Thermoanaerobaculum aquaticum gen. nov., sp. nov., the first cultivated member of Acidobacteria subdivision 23, isolated from a hot spring.
abstract: A novel bacterium was isolated from a freshwater hot spring, the Hale House Spring, located at Hot Springs National Park, Hot Springs, AR, USA. Cells of strain MP-01(T) stained Gram-negative, were rod-shaped, non-motile, strictly anaerobic and chemo-organotrophic and did not form spores. Growth occurred at 50-65 °C, with an optimum at 60 °C, at pH 6.0-8.0, with an optimum at pH 6.5-7.0, and at NaCl concentrations up to 0.5 % (w/v), with optimum growth in the absence of NaCl. Strain MP-01(T) was capable of fermentative growth on pyruvate or proteinaceous substrates as well as reducing Fe(III) and Mn(IV). Major fatty acids were iso-C15 : 0, iso-C16 : 0, anteiso-C17 : 0 and iso-C17 : 0. The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine and the major isoprenoid quinone was MK-10. In the polyamine pattern, sym-homospermidine was the predominant compound. The DNA G+C content was 62.7 mol%. Analysis of the 16S rRNA gene sequence of the isolate indicated that strain MP-01(T) represents the first reported cultivated member of subdivision 23 of the Acidobacteria. It is proposed that strain MP-01(T) represents a novel genus and species, for which the name Thermoanaerobaculum aquaticum gen. nov., sp. nov. is proposed. The type strain of Thermoanaerobaculum aquaticum is MP-01(T) ( = DSM 24856(T) = JCM 18256(T)).
authors.affiliation: Department of Microbiology and Plant Biology, University of Oklahoma, Norman, OK 73019, USA.
authors.forename: Nathaniel A
authors.initials: NA
authors.lastname: Losey
authors.affiliation: Graduate Program in Ecology and Evolutionary Biology, University of Oklahoma, Norman, OK 73019, USA.|Department of Microbiology and Plant Biology, University of Oklahoma, Norman, OK 73019, USA.
authors.forename: Bradley S
authors.initials: BS
authors.lastname: Stevenson
authors.affiliation: Institute of Bacteriology, Mycology and Hygiene, University of Veterinary Medicine Vienna, Veterinärplatz 1, A-1210 Vienna, Austria.
authors.forename: Hans-Jürgen
authors.initials: HJ
authors.lastname: Busse
authors.affiliation: NIOZ Royal Netherlands Institute for Sea Research, Department of Marine Organic Biogeochemistry, PO Box 59, 1790 AB Den Burg, Netherlands.
authors.forename: Jaap S Sinninghe
authors.initials: JSS
authors.lastname: Damsté
authors.affiliation: NIOZ Royal Netherlands Institute for Sea Research, Department of Marine Organic Biogeochemistry, PO Box 59, 1790 AB Den Burg, Netherlands.
authors.forename: W Irene C
authors.initials: WIC
authors.lastname: Rijpstra
authors.affiliation: Hot Springs National Park, Hot Springs, AR 71901, USA.
authors.forename: Stephen
authors.initials: S
authors.lastname: Rudd
authors.affiliation: Graduate Program in Ecology and Evolutionary Biology, University of Oklahoma, Norman, OK 73019, USA.|Department of Microbiology and Plant Biology, University of Oklahoma, Norman, OK 73019, USA.
authors.forename: Paul A
authors.initials: PA
authors.lastname: Lawson
mesh_terms: D061271:Acidobacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D001131:Arkansas; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D005618:Fresh Water / Q000382:microbiology; D045482:Hot Springs / Q000382:microbiology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013095:Spermidine / Q000031:analogs & derivatives / Q000737:chemistry; D014451:Ubiquinone / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; C007961:sym-homospermidine; D014451:Ubiquinone; C025204:Ubiquinone Q2; D013095:Spermidine