title: The dendritic spines of interneurons are dynamic structures influenced by PSA-NCAM expression.
abstract: Excitatory neurons undergo dendritic spine remodeling in response to different stimuli. However, there is scarce information about this type of plasticity in interneurons. The polysialylated form of the neural cell adhesion molecule (PSA-NCAM) is a good candidate to mediate this plasticity as it participates in neuronal remodeling and is expressed by some mature cortical interneurons, which have reduced dendritic arborization, spine density, and synaptic input. To study the connectivity of the dendritic spines of interneurons and the influence of PSA-NCAM on their dynamics, we have analyzed these structures in a subpopulation of fluorescent spiny interneurons in the hippocampus of glutamic acid decarboxylase-enhanced green fluorescent protein transgenic mice. Our results show that these spines receive excitatory synapses. The depletion of PSA in vivo using the enzyme Endo-Neuraminidase-N (Endo-N) increases spine density when analyzed 2 days after, but decreases it 7 days after. The dendritic spine turnover was also analyzed in real time using organotypic hippocampal cultures: 24 h after the addition of EndoN, we observed an increase in the apparition rate of spines. These results indicate that dendritic spines are important structures in the control of the synaptic input of hippocampal interneurons and suggest that PSA-NCAM is relevant in the regulation of their morphology and connectivity.
authors.affiliation: Cell Biology Department, Neurobiology Unit and Program in Basic and Applied Neurosciences, Universitat de València, Valencia, Spain Current address: Neuroscience Center, University of Helsinki, Helsinki, Finland.
authors.forename: Ramon
authors.initials: R
authors.lastname: Guirado
authors.affiliation: Cell Biology Department, Neurobiology Unit and Program in Basic and Applied Neurosciences, Universitat de València, Valencia, Spain.
authors.forename: Marta
authors.initials: M
authors.lastname: Perez-Rando
authors.affiliation: Cell Biology Department, Neurobiology Unit and Program in Basic and Applied Neurosciences, Universitat de València, Valencia, Spain.
authors.forename: David
authors.initials: D
authors.lastname: Sanchez-Matarredona
authors.affiliation: Cell Biology Department, Neurobiology Unit and Program in Basic and Applied Neurosciences, Universitat de València, Valencia, Spain.
authors.forename: Esther
authors.initials: E
authors.lastname: Castillo-Gómez
authors.affiliation: Cell Biology Department, Neurobiology Unit and Program in Basic and Applied Neurosciences, Universitat de València, Valencia, Spain.
authors.forename: Teresa
authors.initials: T
authors.lastname: Liberia
authors.affiliation: Cell Biology Department, Neurobiology Unit and Program in Basic and Applied Neurosciences, Universitat de València, Valencia, Spain.
authors.forename: Laura
authors.initials: L
authors.lastname: Rovira-Esteban
authors.affiliation: Cell Biology Department, Neurobiology Unit and Program in Basic and Applied Neurosciences, Universitat de València, Valencia, Spain.
authors.forename: Emilio
authors.initials: E
authors.lastname: Varea
authors.affiliation: Cell Biology Department, Neurobiology Unit and Program in Basic and Applied Neurosciences, Universitat de València, Valencia, Spain.
authors.forename: Carlos
authors.initials: C
authors.lastname: Crespo
authors.affiliation: Cell Biology Department, Neurobiology Unit and Program in Basic and Applied Neurosciences, Universitat de València, Valencia, Spain.
authors.forename: José Miguel
authors.initials: JM
authors.lastname: Blasco-Ibáñez
authors.affiliation: Cell Biology Department, Neurobiology Unit and Program in Basic and Applied Neurosciences, Universitat de València, Valencia, Spain Fundación Hospital Clínico Universitario de Valencia, INCLIVA, Valencia, Spain CIBERSAM, Spanish National Network for Research in Mental Health, Madrid, Spain.
authors.forename: Juan
authors.initials: J
authors.lastname: Nacher
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D064032:Calbindin 2 / Q000378:metabolism; D002766:Cholecystokinin / Q000378:metabolism; D049229:Dendritic Spines / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D005786:Gene Expression Regulation / Q000187:drug effects / Q000502:physiology*; D005968:Glutamate Decarboxylase / Q000235:genetics; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006624:Hippocampus / Q000166:cytology; D066298:In Vitro Techniques; D007395:Interneurons / Q000187:drug effects / Q000648:ultrastructure*; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D009419:Nerve Tissue Proteins / Q000378:metabolism; D039842:Neural Cell Adhesion Molecule L1 / Q000187:drug effects / Q000378:metabolism* / Q000502:physiology*; D009439:Neuraminidase / Q000494:pharmacology; D009924:Organ Culture Techniques; D012794:Sialic Acids / Q000378:metabolism* / Q000502:physiology*; D013004:Somatostatin / Q000378:metabolism; D013997:Time Factors; D014660:Vasoactive Intestinal Peptide / Q000378:metabolism
chemical_list: D064032:Calbindin 2; D009419:Nerve Tissue Proteins; D039842:Neural Cell Adhesion Molecule L1; D012794:Sialic Acids; C103469:polysialyl neural cell adhesion molecule; D049452:Green Fluorescent Proteins; D014660:Vasoactive Intestinal Peptide; D013004:Somatostatin; D002766:Cholecystokinin; D009439:Neuraminidase; D005968:Glutamate Decarboxylase
keywords: dendritic spine; hippocampus; interneuron; polysialic acid; structural plasticity