title: Molecular epidemiology of PRRSV in South China from 2007 to 2011 based on the genetic analysis of ORF5.
abstract: Porcine reproductive and respiratory syndrome virus (PRRSV) has proven to be highly genetically variable; however, comprehensive information regarding the virus's genetic diversity in South China is limited. In this study, a total of 3199 clinical samples were collected from 267 pig farms suspected of PRRSV infection between 2007 and 2011. The ORF5 genes of 51 PRRSV-positive samples were sequenced and analyzed. The 51 study strains were divided into three primary subgenotypes. Fourty-five of the strains belonged to subgenotype I and were closely related to the highly pathogenic PRRSV (HP-PRRSV) strains. The subgenotype I strains were generally clustered into genetically similar groups by year. Only one of the strains belonged to subgenotype II, clustering with the classical North American type, VR2332. Five of the strains were grouped into subgenotype III, which occupied a separate branch and was closely related to the recently isolated novel field strains, QYYZ and GM2. The 5 subgenotype III strains shared an amino acid identity with the remaining 46 study strains ranging from 79.6%-83.6%. Amino acid analysis showed extensive mutations in subgenotype III; the diverse genetic mutations of these novel strains are of great concern.
authors.affiliation: MOA Key Laboratory of Animal Vaccine Development, Ministry of China, College of Veterinary Medicine, South China Agricultural University, 483 Wushan Road, Tianhe District, Guangzhou 510642, China.
authors.forename: Jiexiong
authors.initials: J
authors.lastname: Xie
authors.forename: Wanjun
authors.initials: W
authors.lastname: Zhu
authors.forename: Ye
authors.initials: Y
authors.lastname: Chen
authors.forename: Chunya
authors.initials: C
authors.lastname: Wei
authors.forename: Pei
authors.initials: P
authors.lastname: Zhou
authors.forename: Minze
authors.initials: M
authors.lastname: Zhang
authors.forename: Zhen
authors.initials: Z
authors.lastname: Huang
authors.forename: Long
authors.initials: L
authors.lastname: Sun
authors.forename: Shuo
authors.initials: S
authors.lastname: Su
authors.forename: Guihong
authors.initials: G
authors.lastname: Zhang
mesh_terms: D000818:Animals; D002681:China / Q000453:epidemiology; D016000:Cluster Analysis; D005838:Genotype; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D019318:Porcine Reproductive and Respiratory Syndrome / Q000453:epidemiology* / Q000821:virology*; D019316:Porcine respiratory and reproductive syndrome virus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D012367:RNA, Viral / Q000235:genetics; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013552:Swine; D014759:Viral Envelope Proteins / Q000235:genetics*
chemical_list: D012367:RNA, Viral; D014759:Viral Envelope Proteins; C114789:glycoprotein 5, PRRSV
keywords: GP5; Genetic diversity; Porcine reproductive and respiratory syndrome virus (PRRSV); South China