title: Volatile biomarkers from human melanoma cells.
abstract: Dogs can identify, by olfaction, melanoma on the skin of patients or melanoma samples hidden on healthy subjects, suggesting that volatile organic compounds (VOCs) from melanoma differ from those of normal skin. Studies employing gas chromatography-mass spectrometry (GC-MS) and gas sensors reported that melanoma-related VOCs differed from VOCs from normal skin sources. However, the identities of the VOCs that discriminate melanoma from normal skin were either unknown or likely derived from exogenous sources. We employed solid-phase micro-extraction, GC-MS and single-stranded DNA-coated nanotube (DNACNT) sensors to examine VOCs from melanoma and normal melanocytes. GC-MS revealed dozens of VOCs, but further analyses focused on compounds most likely of endogenous origin. Several compounds differed between cancer and normal cells, e.g., isoamyl alcohol was higher in melanoma cells than in normal melanocytes but isovaleric acid was lower in melanoma cells. These two compounds share the same precursor, viz., leucine. Melanoma cells produce dimethyldi- and trisulfide, compounds not detected in VOCs from normal melanocytes. Furthermore, analyses of the total volatile metabolome from both melanoma cells and normal melanocytes by DNACNT sensors, coupled with the GC-MS results, demonstrate clear differences between these cell systems. Consequently, monitoring of melanoma VOCs has potential as a useful screening methodology.
authors.affiliation: Monell Chemical Senses Center, 3500 Market Street, Philadelphia, PA 19104, USA.
authors.forename: Jae
authors.initials: J
authors.lastname: Kwak
authors.forename: Michelle
authors.initials: M
authors.lastname: Gallagher
authors.forename: Mehmet Hakan
authors.initials: MH
authors.lastname: Ozdener
authors.forename: Charles J
authors.initials: CJ
authors.lastname: Wysocki
authors.forename: Brett R
authors.initials: BR
authors.lastname: Goldsmith
authors.forename: Amaka
authors.initials: A
authors.lastname: Isamah
authors.forename: Adam
authors.initials: A
authors.lastname: Faranda
authors.forename: Steven S
authors.initials: SS
authors.lastname: Fakharzadeh
authors.forename: Meenhard
authors.initials: M
authors.lastname: Herlyn
authors.forename: A T Charlie
authors.initials: AT
authors.lastname: Johnson
authors.forename: George
authors.initials: G
authors.lastname: Preti
mesh_terms: D014408:Biomarkers, Tumor / Q000032:analysis* / Q000378:metabolism; D002460:Cell Line; D045744:Cell Line, Tumor; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008544:Melanocytes / Q000737:chemistry / Q000166:cytology / Q000378:metabolism; D008545:Melanoma / Q000737:chemistry* / Q000378:metabolism / Q000473:pathology; D037742:Nanotubes, Carbon / Q000737:chemistry; D015203:Reproducibility of Results; D012878:Skin Neoplasms / Q000737:chemistry / Q000378:metabolism / Q000473:pathology; D055549:Volatile Organic Compounds / Q000032:analysis* / Q000378:metabolism
chemical_list: D014408:Biomarkers, Tumor; D037742:Nanotubes, Carbon; D055549:Volatile Organic Compounds