title: Embryos produced from fertilization with bovine viral diarrhea virus (BVDV)-infected semen and the risk of disease transmission to embryo transfer (ET) recipients and offspring.
abstract: Bovine diarrhea virus (BVDV) causes a variety of economically important enteric and infertility problems in cattle. For that reason, several countries have eradicated the disease, and some others have schemes in progress to achieve freedom. Although there is a considerable amount of information about the risk of BVDV transmission through contaminated semen used for artificial insemination (AI), there is no evidence to indicate whether the resulting embryos, when used for embryo transfer, can lead to the transmission of BVDV to recipients or offspring. For this experiment, semen from a bull persistently infected with BVDV (10(5) 50% tissue culture infective doses/mL NY strain) was used for insemination (two times at estrus) of BVDV-seronegative, superovulated cows (N = 35). Embryos were collected 7 days after insemination and subsequently were washed according to the International Embryo Transfer Society recommendations or left unwashed. Out of 302 collected oocytes and embryos, 173 (57%) were fertilized and the remaining 129 (43%) had degenerated. Infectious BVDV was detected in 24% (17/71) of unwashed and 10% (8/77) of washed embryos, and in all (N = 11) follicular fluid samples, oviductal epithelial cells, endometrium, and corpora lutea tissues as determined by the virus isolation test. After transfer of 39 washed embryos to 27 BVDV-seronegative recipients, 12 (44%) cows became pregnant and 17 calves free of BVDV and BVDV antibodies, including five sets of twins, were born. After embryo transfer, all pregnant and nonpregnant recipients remained free of BVDV and antibodies. In conclusion, results herein suggest that BVDV can be transmitted by AI resulting in the production of some proportion of contaminated embryos. However, it appears that such embryos, when washed according to International Embryo Transfer Society and the World Organization for Animal Health guidelines do not cause BVDV transmission to recipients or their offspring.
authors.affiliation: Canadian Food Inspection Agency, Animal Health Microbacteriology Laboratory (Germplasm), Ottawa, Ontario, Canada. Andrzej.Bielanski@inspection.gc.ca
authors.forename: A
authors.initials: A
authors.lastname: Bielanski
authors.forename: J
authors.initials: J
authors.lastname: Algire
authors.forename: A
authors.initials: A
authors.lastname: Lalonde
authors.forename: A
authors.initials: A
authors.lastname: Garceac
mesh_terms: D000818:Animals; D001912:Bovine Virus Diarrhea-Mucosal Disease / Q000517:prevention & control / Q000635:transmission*; D002417:Cattle; D001908:Diarrhea Viruses, Bovine Viral; D018562:Disease Transmission, Infectious; D004624:Embryo Transfer / Q000662:veterinary*; D004622:Embryo, Mammalian / Q000821:virology*; D005260:Female; D015571:Follicular Fluid / Q000821:virology; D007315:Insemination, Artificial / Q000009:adverse effects / Q000662:veterinary; D011247:Pregnancy; D018570:Risk Assessment; D012661:Semen / Q000821:virology*
keywords: BVDV; Embryo; Embryo transfer; Fertilization; IVF; Semen