title: Simplification and improvement of protein detection in two-dimensional electrophoresis gels with SERVA HPE™ lightning red.
abstract: A new fluorescent amino-reactive dye has been tested for both labelling proteins prior to electrophoretic separations and between the two steps of two-dimensional electrophoresis. A series of experiments showed, that the labelling of lysines with this dye is compatible with all standard additives used for sample preparation, including reducing substances and carrier ampholytes. Using this dye for pre-labelling considerably simplifies the electrophoresis and detection workflow and provides highly sensitive and quantitative visualisation of proteins.
authors.affiliation: SERVA Electrophoresis GmbH, Heidelberg, Germany.
authors.forename: Anja
authors.initials: A
authors.lastname: Griebel
authors.forename: Christian
authors.initials: C
authors.lastname: Obermaier
authors.forename: Reiner
authors.initials: R
authors.lastname: Westermeier
authors.forename: Martin
authors.initials: M
authors.lastname: Moche
authors.forename: Knut
authors.initials: K
authors.lastname: Büttner
mesh_terms: D001412:Bacillus subtilis / Q000737:chemistry; D001426:Bacterial Proteins / Q000032:analysis*; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D004926:Escherichia coli / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry*; D007091:Image Processing, Computer-Assisted; D007525:Isoelectric Focusing; D008239:Lysine / Q000737:chemistry; D040901:Proteomics / Q000379:methods*; D012680:Sensitivity and Specificity
chemical_list: D001426:Bacterial Proteins; D005456:Fluorescent Dyes; D008239:Lysine