title: Effects of supplementing different ratios of omega-3 and omega-6 fatty acids in western-style diets on cow's milk protein allergy in a mouse model.
abstract: METHODS
  This study investigated the effects of supplementing different ratios of omega-6 and omega-3 fatty acids (O6H = 10:1, O3O6 = 4:1, and O3H = 1:4) to western-style diets on cow β-lactoglobulin (BLG) induced allergic reactions in Balb/c mice.
  
  
  RESULTS
  Three-week-old mice were randomly assigned to three diet groups (n = 20/group). At 9 wk of age, half of the mice from each dietary treatment (n = 10) were intraperitoneally (i.p.) sensitized with three weekly doses of BLG and alum while the remaining half from each group was sham sensitized (controls). One week after the final sensitization, all mice were orally challenged with BLG. Elevated BLG-specific serum Igs were observed in all sensitized and challenged mice. IFN-γ, MCP-1, and IL-12p40 concentrations from lymphocytes of mesenteric lymph nodes were highest in O3H mice, compared to O3O6 and O6H mice. O6H mice had the highest IL-4 concentrations from splenic lymphocytes and a significantly lower rectal temperature after the challenge in comparison to O3O6 and O3H mice.
  
  
  CONCLUSIONS
  Our results suggest that the ω-3 PUFA rich diets alleviated the severity of allergic reactions, and may modulate immune response toward T helper cell (Th)1-favoured immune response while the ω-6 PUFA rich diet exhibited no allergy alleviation with a stronger Th2 polarized immune response.
authors.affiliation: Department of Animal Science, Macdonald Campus, McGill University, Lakeshore, Quebec, Canada.
authors.forename: Cin L
authors.initials: CL
authors.lastname: Thang
authors.forename: Joyce I
authors.initials: JI
authors.lastname: Boye
authors.forename: Hai Ning
authors.initials: HN
authors.lastname: Shi
authors.forename: Xin
authors.initials: X
authors.lastname: Zhao
mesh_terms: D000818:Animals; D051792:Basic Helix-Loop-Helix Proteins / Q000276:immunology / Q000378:metabolism; D018932:Chemokine CCL2 / Q000097:blood / Q000276:immunology; D004032:Diet; D019587:Dietary Supplements; D004195:Disease Models, Animal; D015525:Fatty Acids, Omega-3 / Q000008:administration & dosage* / Q000097:blood; D043371:Fatty Acids, Omega-6 / Q000008:administration & dosage* / Q000097:blood; D005260:Female; D007070:Immunoglobulin A / Q000097:blood / Q000276:immunology; D007073:Immunoglobulin E / Q000097:blood / Q000276:immunology; D007371:Interferon-gamma / Q000097:blood / Q000276:immunology; D018664:Interleukin-12 / Q000097:blood / Q000276:immunology; D015847:Interleukin-4 / Q000276:immunology / Q000378:metabolism; D007782:Lactoglobulins / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D016269:Milk Hypersensitivity / Q000276:immunology / Q000517:prevention & control*; D013154:Spleen / Q000276:immunology / Q000378:metabolism
chemical_list: D051792:Basic Helix-Loop-Helix Proteins; D018932:Chemokine CCL2; D015525:Fatty Acids, Omega-3; D043371:Fatty Acids, Omega-6; D007070:Immunoglobulin A; D007073:Immunoglobulin E; D007371:Interferon-gamma; D018664:Interleukin-12; D015847:Interleukin-4; D007782:Lactoglobulins; C491768:Ccl2 protein, mouse; C095572:Hand1 protein, mouse
keywords: Food allergy; Mouse model; Omega 3 fatty acids; Western-style diet; β-Lactoglobulin