title: The structure and immunoreactivity of exopolysaccharide isolated from Lactobacillus johnsonii strain 151.
abstract: The exopolysaccharide (EPS) structure from Lactobacillus johnsonii strain 151 isolated from the intestinal tract of mice was investigated. Sugar and methylation analyses together with (1)H and (13)C NMR spectroscopy, including two-dimensional (1)H,(1)H COSY, TOCSY, NOESY, and (1)H,(13)C HSQC, HMBC experiments, revealed that the repeating unit of the EPS is the linear pentasaccharide: →6)-α-d-Galp-(1→6)-α-d-Glcp-(1→3)-β-d-Galf-(1→3)-α-d-Glcp-(1→2)-β-d-Galf-(1→ The immunoreactivity of two structurally different exopolysaccharides isolated from L. johnsonii, 151 and 142 (Carbohydr. Res. 2010, 345, 108-114), was compared. Both EPSs differed in their reactivity with antisera. EPS from L. johnsonii 151 reacted with anti-Lactobacillus polyclonal sera against cells of five different strains, while EPS from L. johnsonii 142 was found to react only with its own antiserum. The broader specificity and higher reactivity of EPS from 151 strain than EPS from 142 strain were also observed with human sera. The physiological antibodies recognizing polysaccharide antigens were present in both adults and umbilical cord blood sera. A highly specific EPS 142 bearing strain was isolated from experimentally induced inflammatory bowel disease (IBD) mice, while a strain with EPS 151 isolated from the intestinal tract of healthy mice is characterized by a broad immune reactivity common structure.
authors.affiliation: Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Weigla 12, 53-114 Wrocław, Poland. sabina.gorska@iitd.pan.wroc.pl
authors.forename: Sabina
authors.initials: S
authors.lastname: Górska-Frączek
authors.forename: Corine
authors.initials: C
authors.lastname: Sandström
authors.forename: Lennart
authors.initials: L
authors.lastname: Kenne
authors.forename: Mariola
authors.initials: M
authors.lastname: Paściak
authors.forename: Ewa
authors.initials: E
authors.lastname: Brzozowska
authors.forename: Magdalena
authors.initials: M
authors.lastname: Strus
authors.forename: Piotr
authors.initials: P
authors.lastname: Heczko
authors.forename: Andrzej
authors.initials: A
authors.lastname: Gamian
mesh_terms: D000328:Adult; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood / Q000276:immunology; D002240:Carbohydrate Sequence; D006801:Humans; D007778:Lactobacillus / Q000737:chemistry* / Q000276:immunology; D051379:Mice; D008969:Molecular Sequence Data; D011135:Polysaccharides, Bacterial / Q000737:chemistry* / Q000276:immunology* / Q000302:isolation & purification; D011817:Rabbits; D013045:Species Specificity
chemical_list: D000907:Antibodies, Bacterial; D011135:Polysaccharides, Bacterial
keywords: 3,3′,5,5′-tetramethylbenzidine; DEAE; EPS; Exopolysaccharide structure; Immunoreactivity; LAB; Lactobacillus; NMR; TCA; TFA; TMB; diethylaminoethyl; exopolysaccharide; lactic acid bacteria; trichloroacetic acid; trifluoroacetic acid