title: Discrimination against major groove adducts by Y-family polymerases of the DinB subfamily.
abstract: Y-family DNA polymerases bypass DNA adducts in a process known as translesion synthesis (TLS). Y-family polymerases make contacts with the minor groove side of the DNA substrate at the nascent base pair. The Y-family polymerases also contact the DNA major groove via the unique little finger domain, but they generally lack contacts with the major groove at the nascent base pair. Escherichia coli DinB efficiently and accurately copies certain minor groove guanosine adducts. In contrast, we previously showed that the presence in the DNA template of the major groove-modified base 1,3-diaza-2-oxophenothiazine (tC) inhibits the activity of E. coli DinB. Even when the DNA primer is extended up to three nucleotides beyond the site of the tC analog, DinB activity is strongly inhibited. These findings prompted us to investigate discrimination against other major groove modifications by DinB and its orthologs. We chose a set of pyrimidines and purines with modifications in the major groove and determined the activity of DinB and several orthologs with these substrates. DinB, human pol kappa, and Sulfolobus solfataricus Dpo4 show differing specificities for the major groove adducts pyrrolo-dC, dP, N(6)-furfuryl-dA, and etheno-dA. In general, DinB was least efficient for bypass of all of these major groove adducts, whereas Dpo4 was most efficient. DinB activity was essentially completely inhibited by the presence of etheno-dA, while pol kappa activity was strongly inhibited. All three of these DNA polymerases were able to bypass N(6)-furfuryl-dA with modest efficiency, with DinB being the least efficient. We also determined that the R35A variant of DinB enhances bypass of N(6)-furfuryl-dA but not etheno-dA. In sum, we find that whereas DinB is specific for bypass of minor groove adducts, it is specifically inhibited by major groove DNA modifications.
authors.affiliation: Department of Chemistry and Chemical Biology, Northeastern University, Boston, MA 02115, USA.
authors.forename: Jason M
authors.initials: JM
authors.lastname: Walsh
authors.forename: Paul J
authors.initials: PJ
authors.lastname: Ippoliti
authors.forename: Erin A
authors.initials: EA
authors.lastname: Ronayne
authors.forename: Eriks
authors.initials: E
authors.lastname: Rozners
authors.forename: Penny J
authors.initials: PJ
authors.lastname: Beuning
mesh_terms: D001483:Base Sequence; D020134:Catalytic Domain; D018736:DNA Adducts / Q000737:chemistry*; D004259:DNA-Directed DNA Polymerase / Q000737:chemistry; D004926:Escherichia coli / Q000201:enzymology; D029968:Escherichia coli Proteins / Q000037:antagonists & inhibitors / Q000737:chemistry* / Q000235:genetics / Q000502:physiology; D006801:Humans; D007700:Kinetics; D008958:Models, Molecular; D020125:Mutation, Missense; D009690:Nucleic Acid Conformation; D019384:Nucleic Acid Synthesis Inhibitors; D013379:Substrate Specificity; D048229:Sulfolobus solfataricus / Q000201:enzymology; D000099270:Y-Family DNA Polymerases
chemical_list: D018736:DNA Adducts; D004259:DNA-Directed DNA Polymerase; D029968:Escherichia coli Proteins; D019384:Nucleic Acid Synthesis Inhibitors; C094399:DinB protein, E coli; C404302:POLK protein, human; D000099270:Y-Family DNA Polymerases
keywords: 1,N(6)-ethenodeoxyadenosine; E. coli; Escherichia coli; Escherichia coli DinB; Human DNA polymerase kappa; N(6)-Furfuryl-dA; N(6)-furfuryl-dA; S. solfataricus; Sulfolobus solfataricus; Sulfolobus solfataricus Dpo4; Translesion synthesis; etheno-dA; fdA; pol; polymerase; ɛdA