title: Changes in platelet GPIbα and ADAM17 during the acute stage of atherosclerotic ischemic stroke among Chinese.
abstract: Glycoprotein (GP) Ibα ectodomain shedding has important implications for thrombosis and hemostasis. A disintegrin and metalloproteinase 17 (ADAM17) was identified to play an essential role in agonist induced GPIbα shedding. The relationship of GPIbα shedding and ADAM17 in the acute stage of atherosclerotic ischemic stroke (AIS) patients has not been thoroughly studied. A total of 306 patients and 230 controls matched for age, sex, race, history of hypertension and diabetes mellitus were enrolled in the study. GPIbα, ADAM17, glycocalicin were detected by flow cytometry, Western blotting, and enzyme-linked immunosorbent assay (ELISA) respectively. Compared with the control group, the expression of GPIbα in patients with acute ischemic stroke was significantly lower (P=0.000, P<0.01). Plasma glycocalicin and ADAM17 in AIS group were higher than those in control group (P=0.699, P=0.000). Pearson's analysis showed glycocalicin bore no correlation with GPIbα in AIS patients (r=0.095, P>0.05). GPIbα and National Institute of Health Stroke Scale (NIHSS) had negative correlation (r=-0.514, P<0.01). Our findings indicate that ADAM17 may be a risk factor for ischemic stroke in Chinese and the expression of GPIbα can serve as a measure for stroke severity.
authors.affiliation: Department of Integrated Chinese and Western Medicine, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China.|Department of Chinese Medicine, the First Hospital of Wuhan, Wuhan, 430022, China.
authors.forename: Jia-Yan
authors.initials: JY
authors.lastname: Ling
authors.affiliation: Department of Integrated Chinese and Western Medicine, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China. shenlinhb@sina.cn.
authors.forename: Lin
authors.initials: L
authors.lastname: Shen
authors.affiliation: Laboratory Department, Chinese Medicine Hospital of Hubei Province, Wuhan, 430061, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Liu
authors.affiliation: Department of Chinese Medicine, the First Hospital of Wuhan, Wuhan, 430022, China.
authors.forename: Sha
authors.initials: S
authors.lastname: Xue
authors.affiliation: Laboratory Department, the First Hospital of Wuhan, Wuhan, 430022, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Ma
authors.affiliation: Department of Neurology, the First Hospital of Wuhan, Wuhan, 430022, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Wu
authors.affiliation: Laboratory Department, the First Hospital of Wuhan, Wuhan, 430022, China.
authors.forename: Zi-Xi
authors.initials: ZX
authors.lastname: Li
authors.affiliation: Department of Integrated Chinese and Western Medicine, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Zhu
mesh_terms: D051722:ADAM Proteins / Q000097:blood*; D000072198:ADAM17 Protein; D015415:Biomarkers / Q000097:blood; D001792:Blood Platelets / Q000378:metabolism*; D002545:Brain Ischemia / Q000097:blood* / Q000175:diagnosis; D002681:China; D005260:Female; D006801:Humans; D002537:Intracranial Arteriosclerosis / Q000097:blood* / Q000175:diagnosis; D008297:Male; D008875:Middle Aged; D019038:Platelet Glycoprotein GPIb-IX Complex / Q000378:metabolism*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012720:Severity of Illness Index; D020521:Stroke / Q000097:blood* / Q000175:diagnosis
chemical_list: D015415:Biomarkers; D019038:Platelet Glycoprotein GPIb-IX Complex; C094990:adhesion receptor; D051722:ADAM Proteins; D000072198:ADAM17 Protein; C000606462:ADAM17 protein, human