title: Bisphenol A at concentrations relevant to human exposure enhances histamine and cysteinyl leukotriene release from bone marrow-derived mast cells.
abstract: Bisphenol A (BPA), a monomer of polycarbonate plastics and epoxide resin, acts as an endocrine-active compound and has been shown to enhance the inflammatory response to allergen challenge. Previous reports in rodents have demonstrated that perinatal BPA exposure alters airway inflammation following sensitization and challenge to ovalbumin in juvenile and adult offspring. Additionally, a high concentration of BPA has been shown to enhance mediator release in mast cell lines. This study aimed to determine if short-term BPA exposure, at levels relevant to human exposure, enhances mast cell release of histamine and cysteinyl leukotrienes (CysLTs). Primary murine bone marrow-derived mast cells (BMMC) produced from the femurs of female C57BL/6 mice were stimulated with BPA or estradiol (E2) in vitro. It was observed that both BPA and E2 increased BMMC histamine release over a range of nanomolar concentrations (1-1000 nM). The estrogen receptor (ER) antagonist ICI 182,780 partially blocked the ability of E2, but not BPA, to elevate histamine release. BPA also increased CysLT release, which was not abrogated by ER inhibition. It was also observed that the ability of BPA to enhance histamine and CysLT release was inhibited by blocking the extracellular signal-regulated kinase (ERK) pathway with U0126 or by chelating extracellular calcium (Ca(2+)) using EGTA. In summary, these experiments are the first to demonstrate that acute BPA exposure enhances mast cell histamine and CysLT release in vitro--an effect that is not dependent on an ER-mediated mechanism. Instead, BPA-induced mast cell histamine and CysLT release may be mediated, in part, by the ERK pathway and extracellular Ca(2+) concentrations. These data suggest that exposure to BPA at levels relevant to human exposure may provoke an acute inflammatory response in atopic individuals via enhanced mast cell activation.
authors.affiliation: Department of Environmental Health Sciences, School of Public Health, University of Michigan , Ann Arbor, MI , USA.
authors.forename: Edmund
authors.initials: E
authors.lastname: O'Brien
authors.forename: Dana C
authors.initials: DC
authors.lastname: Dolinoy
authors.forename: Peter
authors.initials: P
authors.lastname: Mancuso
mesh_terms: D000818:Animals; D001559:Benzhydryl Compounds / Q000276:immunology / Q000378:metabolism*; D001854:Bone Marrow Cells / Q000166:cytology; D002070:Butadienes / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism; D002478:Cells, Cultured; D004853:Epoxy Resins / Q000378:metabolism*; D004958:Estradiol / Q000031:analogs & derivatives / Q000276:immunology / Q000494:pharmacology; D047628:Estrogen Receptor alpha / Q000037:antagonists & inhibitors; D048049:Extracellular Signal-Regulated MAP Kinases / Q000037:antagonists & inhibitors; D005260:Female; D000077267:Fulvestrant; D006632:Histamine / Q000378:metabolism; D006801:Humans; D017998:Leukotriene D4 / Q000378:metabolism; D008407:Mast Cells / Q000166:cytology / Q000276:immunology / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D009570:Nitriles / Q000494:pharmacology; D010636:Phenols / Q000276:immunology / Q000378:metabolism*; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D002070:Butadienes; D002118:Calcium; D004853:Epoxy Resins; D004958:Estradiol; D047628:Estrogen Receptor alpha; D048049:Extracellular Signal-Regulated MAP Kinases; D000077267:Fulvestrant; D006632:Histamine; D017998:Leukotriene D4; D009570:Nitriles; D010636:Phenols; C113580:U 0126; C006780:bisphenol A; D000098826:Bisphenol A Compounds