title: B cell translocation gene, a direct target of miR-142-5p, inhibits vascular smooth muscle cell proliferation by down-regulating cell cycle progression.
abstract: Vascular smooth muscle cell (VSMC) proliferation plays a key role in neointimal hyperplasia and restenosis. Here we report the role of the microRNA miR-142-5p and its downstream target genes on the proliferation of cultured VSMCs. miR-142-5p promoted VSMC proliferation by down-regulating B cell translocation gene 3 (BTG3). We found that BTG3 inhibited the expression of cell cycle regulatory genes and cell growth. As shown by luciferase reporter assay, miR-142-5p bound directly to the 3'-untranslated region of BTG3. Overexpression of miR-142-5p induced expression of cell cycle regulatory genes. Thus, BTG3, a novel, direct target of miR-142-5p, negatively regulates VSMC proliferation.
authors.affiliation: Heart Research Center of Chonnam National University Hospital, Gwangju 501-757, Republic of Korea. sshjkee@hanmail.net
authors.forename: Hae Jin
authors.initials: HJ
authors.lastname: Kee
authors.forename: Sangha
authors.initials: S
authors.lastname: Park
authors.forename: Jin-Sook
authors.initials: JS
authors.lastname: Kwon
authors.forename: Nakwon
authors.initials: N
authors.lastname: Choe
authors.forename: Youngkeun
authors.initials: Y
authors.lastname: Ahn
authors.forename: Hyun
authors.initials: H
authors.lastname: Kook
authors.forename: Myung Ho
authors.initials: MH
authors.lastname: Jeong
mesh_terms: D000818:Animals; D002453:Cell Cycle / Q000502:physiology*; D049109:Cell Proliferation; D002478:Cells, Cultured; D015536:Down-Regulation; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D009131:Muscle, Smooth, Vascular / Q000166:cytology*; D011506:Proteins / Q000235:genetics / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: C402633:Btg3 protein, rat; C583884:MIRN142 microRNA, rat; D035683:MicroRNAs; D011506:Proteins
keywords: BTG3; Neointima hyperplasia; Proliferation; Vascular smooth muscle cell; miR-142-5p