title: A novel density control device for the study of cancer cell autocrine effect.
abstract: In this paper, we propose a novel cell self-loading and patterning device for quantitatively study density effect on cell behaviors. Using this device, it is easy to gather different cell density colonies in different sizes of micro-chambers using one homogeneous cell solution. As a demonstration, we show that the cell number of self-patterning MCF-7 colony is in proportion to the size of liquid-absorbing cavity in the device, from single cell to tens of cells. This device can easily be used to compare the cancer cells' proliferation in different micro-environments, such as the same number of cells in micro-cavities with different sizes, or different numbers of cells in micro-cavities with the same size, or with different FBS concentrations. Our studies imply a plausible positive correlation between the local concentration of autocrine factors and tumor cell proliferation, which is also quantitative analyzed by a simple model.
authors.affiliation: The State Key Laboratory for Artificial Microstructures and Mesoscopic Physics, School of Physics, Peking University, Beijing, 100871, People's Republic of China.|Center for Quantitative Biology, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing, 100871, People's Republic of China.
authors.forename: Wei
authors.initials: W
authors.lastname: Yang
authors.affiliation: Center for Quantitative Biology, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing, 100871, People's Republic of China.
authors.forename: Zhaojun
authors.initials: Z
authors.lastname: Li
authors.affiliation: Peking-Tsinghua Center for Life Sciences, Peking University, Beijing, 100871, People's Republic of China.
authors.forename: Weilin
authors.initials: W
authors.lastname: Zhang
authors.affiliation: The State Key Laboratory for Artificial Microstructures and Mesoscopic Physics, School of Physics, Peking University, Beijing, 100871, People's Republic of China. pkuluocx@pku.edu.cn.|Center for Quantitative Biology, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing, 100871, People's Republic of China. pkuluocx@pku.edu.cn.
authors.forename: Chunxiong
authors.initials: C
authors.lastname: Luo
authors.affiliation: The State Key Laboratory for Artificial Microstructures and Mesoscopic Physics, School of Physics, Peking University, Beijing, 100871, People's Republic of China. qi@pku.edu.cn.|Center for Quantitative Biology, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing, 100871, People's Republic of China. qi@pku.edu.cn.|Peking-Tsinghua Center for Life Sciences, Peking University, Beijing, 100871, People's Republic of China. qi@pku.edu.cn.
authors.forename: Qi
authors.initials: Q
authors.lastname: Ouyang
authors.affiliation: State Key Laboratory of Nuclear Physics and Technology, Peking University, Beijing, 100871, People's Republic of China.
authors.forename: Gen
authors.initials: G
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Nuclear Physics and Technology, Peking University, Beijing, 100871, People's Republic of China.
authors.forename: Yugang
authors.initials: Y
authors.lastname: Wang
mesh_terms: D019898:Autocrine Communication; D002452:Cell Count; D049109:Cell Proliferation; D003470:Culture Media / Q000737:chemistry; D061986:MCF-7 Cells; D055616:Microtechnology / Q000295:instrumentation*
chemical_list: D003470:Culture Media