title: Proximity probing assays for simultaneous visualization of protein complexes in situ.
abstract: EVALUATION OF: Leuchowius KJ, Clausson CM, Grannas K et al. Parallel visualization of multiple protein complexes in individual cells in tumor tissue. Mol. Cell Proteomics doi:10.1074/mcp.O112.023374 (2013) (Epub ahead of print). Techniques for in situ detection and quantification of proteins in fixed tissue remain an important element of both basic biological analyses and clinical biomarker research. The practical importance of such techniques can be exemplified by the everyday clinical use of immunohistochemical detection of the estrogen receptor and HER2 in tissues from breast cancer patients. Several techniques are currently available for detection of single proteins and post-translational modifications, but very few are suitable for detection of protein complexes. Methods that enable simultaneous detection and quantification of protein complexes provide novel possibilities for understanding the biological role(s) of protein complexes and may open new opportunities to improve clinical biomarker research. Leuchowius et al. describe an improved proximity ligation assay for in situ detection of protein complexes, which is able to detect and quantify several protein complexes simultaneously in the same tissue specimen.
authors.affiliation: Sino-Danish Breast Cancer Research Centre and Danish Centre for Translational Breast Cancer Research, Section of Molecular Disease Biology, Department of Veterinary Disease Biology, Faculty of Health and Medical Sciences, University of Copenhagen, Dyrlægevej 88, 1870 Frederiksberg C, Denmark.
authors.forename: José Manuel Afonso
authors.initials: JM
authors.lastname: Moreira
authors.forename: Stine Buch
authors.initials: SB
authors.lastname: Thorsen
authors.forename: Nils
authors.initials: N
authors.lastname: Brünner
authors.forename: Jan
authors.initials: J
authors.lastname: Stenvang
mesh_terms: D000818:Animals; D001943:Breast Neoplasms / Q000235:genetics* / Q000648:ultrastructure*; D066246:ErbB Receptors / Q000235:genetics*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D057054:Molecular Imaging / Q000379:methods*; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000235:genetics*; D020893:Receptor, ErbB-3 / Q000235:genetics*
chemical_list: D066246:ErbB Receptors; D018719:Erb-b2 Receptor Tyrosine Kinases; D020893:Receptor, ErbB-3