title: Genetic Characterization of Methicillin Resistant and Sensitive, Vancomycin Intermediate Staphylococcus aureus Strains Isolated from Different Iranian Hospitals.
abstract: Background. Global concerns have been raised due to upward trend of Vancomycin Intermediate Staphylococcus aureus (VISA) and Vancomycin Resistant Staphylococcus aureus (VRSA) reports which mean casting doubt on the absolute effectiveness of the last line of antibiotic treatment for S. aureus, vancomycin. Hence, epidemiological evaluation can improve global health care policies. Methodology. 171 Isolates of Staphylococcus aureus were collected from different types of clinical samples in selected hospitals in Isfahan, Mashhad, and Tehran, Iran. Then, they were evaluated by agar screening, disk diffusion, and MIC method to determine their resistance to vancomycin and methicillin. The isolated VISA strains were then confirmed with genetic analysis by the evaluation of mecA and vanA genes, SCCmec, agr, and spa type, and also toxin profiles. MLST was also performed. Results and Conclusion. Our data indicated that 67% of isolated S. aureus strains were resistant to methicillin. Furthermore, five isolates (2.9%) had intermediate resistance to vancomycin (VISA). In contrast to usual association of VISA with MRSA strains, we found two isolates of MSSA-VISA. Therefore, our data suggests a probable parallel growing trend of VISA towards MSSA, along with MRSA strains. However, more samples are required to confirm these primarily data. Moreover, genetic analysis of the isolated VISA strains revealed that these strains are endemic Asian clones.
authors.affiliation: Department of Microbiology, School of Medicine, Isfahan University of Medical Sciences, P.O. Box 73461-8174, Isfahan, Iran.
authors.forename: Seyed Asghar
authors.initials: SA
authors.lastname: Havaei
authors.forename: Amir
authors.initials: A
authors.lastname: Azimian
authors.forename: Hosein
authors.initials: H
authors.lastname: Fazeli
authors.forename: Mahmood
authors.initials: M
authors.lastname: Naderi
authors.forename: Kiarash
authors.initials: K
authors.lastname: Ghazvini
authors.forename: Siamak Mirab
authors.initials: SM
authors.lastname: Samiee
authors.forename: Zahra
authors.initials: Z
authors.lastname: Masoumi
authors.forename: Mojtaba
authors.initials: M
authors.lastname: Akbari