title: Protective effect of whey protein hydrolysates on H₂O₂-induced PC12 cells oxidative stress via a mitochondria-mediated pathway.
abstract: Whey protein hydrolysates (WPHs) were prepared with pepsin and trypsin. A PC12 cell model was built to observe the protective effect of WPHs against H2O2-induced oxidative stress. The results indicated that WPHs reduced apoptosis by 14% and increased antioxidant enzyme activities. Flow cytometry was used to assess the accumulation of reactive oxygen species (ROS), Ca(2+) levels and the mitochondrial membrane potential (MMP). The results showed that WPHs suppressed ROS elevation and Ca(2+) levels and stabilised MMP by 16%. The anti-apoptosis/pro-apoptosis proteins Bcl-2/Bax and poly (ADP-ribose) polymerase (PARP) were investigated by Western-blot analysis, which indicated that WPHs increased the expression of Bcl-2 while inhibiting the expression of Bax and the degradation of PARP. WPHs also blocked Caspase-3 activation by 62%. The results demonstrate that WPHs can significantly protect PC12 cells against oxidative stress via a mitochondria-mediated pathway. These findings indicate the potential benefits of WPHs as valuable food antioxidative additives.
authors.affiliation: State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, 1800 Lihu Avenue, Wuxi, Jiangsu 214122, China.
authors.forename: Man-Man
authors.initials: MM
authors.lastname: Jin
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.forename: Hui-Xin
authors.initials: HX
authors.lastname: Yu
authors.forename: Jun
authors.initials: J
authors.lastname: Meng
authors.forename: Zhen
authors.initials: Z
authors.lastname: Sun
authors.forename: Rong-Rong
authors.initials: RR
authors.lastname: Lu
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002118:Calcium / Q000378:metabolism; D002417:Cattle; D015870:Gene Expression / Q000187:drug effects; D006861:Hydrogen Peroxide / Q000378:metabolism; D008894:Milk Proteins / Q000737:chemistry*; D008928:Mitochondria / Q000187:drug effects* / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D016716:PC12 Cells; D011065:Poly(ADP-ribose) Polymerases / Q000235:genetics / Q000378:metabolism; D020011:Protective Agents / Q000737:chemistry / Q000494:pharmacology*; D011492:Protein Hydrolysates / Q000737:chemistry / Q000494:pharmacology*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism; D000067816:Whey Proteins
chemical_list: D008894:Milk Proteins; D020011:Protective Agents; D011492:Protein Hydrolysates; D019253:Proto-Oncogene Proteins c-bcl-2; D017382:Reactive Oxygen Species; D000067816:Whey Proteins; D006861:Hydrogen Peroxide; D011065:Poly(ADP-ribose) Polymerases; D002118:Calcium