title: Preparation of two organoselenium compounds and their induction of apoptosis to SMMC-7221 cells.
abstract: Two organoselenium compounds: xylitol selenious ester (xylitol-Se) and sucrose selenious ester (sucrose-Se) were synthesized, and their molecular structures were characterized in this study. In MTT assay, xylitol-Se and sucrose-Se showed cytostatic effects on human hepatocellular carcinoma cells SMMC-7221 in a dose-dependent manner, whereas they had no negative influences on the proliferation of human normal hepatic cells HL-7702 in the concentration range from 0.15 to 1.2 ppm Se. Morphological observation, agarose gel electrophoresis, and caspase-3 assay indicated that xylitol-Se and sucrose-Se induced mitochondrial apoptosis to SMMC-7221 cells, which is supported by the depletion of mitochondrial membrane potential and suppression of caspase-3 activity, indicating their ability of inducing apoptosis to cancer cells and great potentials as anticancer drugs.
authors.affiliation: Key Laboratory of Pesticide and Chemical Biology (Ministry of Education), College of Chemistry, Central China Normal University, Wuhan 430079, People's Republic of China.
authors.forename: Pei
authors.initials: P
authors.lastname: Guo
authors.forename: Qing
authors.initials: Q
authors.lastname: Wang
authors.forename: Jing
authors.initials: J
authors.lastname: Liu
authors.forename: Lina
authors.initials: L
authors.lastname: Liu
authors.forename: Peiguang
authors.initials: P
authors.lastname: Zhao
authors.forename: Yu
authors.initials: Y
authors.lastname: Cao
authors.forename: Yuping
authors.initials: Y
authors.lastname: Liu
authors.forename: Chao
authors.initials: C
authors.lastname: Qi
authors.forename: Yanli
authors.initials: Y
authors.lastname: Liu
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D006528:Carcinoma, Hepatocellular / Q000188:drug therapy / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D003603:Cytotoxins / Q000737:chemistry / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D006801:Humans; D008113:Liver Neoplasms / Q000188:drug therapy / Q000378:metabolism* / Q000473:pathology; D008930:Mitochondria, Liver / Q000378:metabolism / Q000473:pathology; D051336:Mitochondrial Membranes / Q000378:metabolism / Q000473:pathology; D016566:Organoselenium Compounds / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D000970:Antineoplastic Agents; D003603:Cytotoxins; D016566:Organoselenium Compounds