title: Enhanced growth of recombinant human adenovirus type 41 (HAdV-41) carrying ADP gene.
abstract: Human adenovirus type 41 (HAdV-41) has the potential to be constructed as a gene transfer vector for oral vaccine or gene therapy targeting gastrointestinal tract. Block in release of progeny virus from host cell severely affects the yield during virus amplification. In this study, HAdV-5 adenovirus death protein (ADP) gene was used to replace the open reading frames (ORFs) of the HAdV-41 E3 region to construct a backbone plasmid pAdbone41ADP. Recombinant adenoviral plasmids harboring ADP and GFP genes (pAd41ADP-GFP) were generated. Plaques were formed and HAdV-41-ADP-GFP virus was rescued after transfecting pAd41ADP-GFP into the packaging cell line 293TE32. When amplified on 293TE32 cells, HAdV-41-ADP-GFP virus released to the culture medium was 10-50 times more than control virus HAdV-41-GFP, which did not carry ADP gene. The results demonstrated that incorporation of the ADP gene substantially increased the yield of recombinant HAdV-41 virus through enhancing spread of progeny virus among packaging cells.
authors.affiliation: Department of Infectious Diseases, College of Veterinary Medicine, University of Georgia, GA 30602, USA.
authors.forename: Zhuo-Zhuang
authors.initials: ZZ
authors.lastname: Lu
authors.forename: Xiao-Hui
authors.initials: XH
authors.lastname: Zou
authors.forename: Krista
authors.initials: K
authors.lastname: Lastinger
authors.forename: Ashley
authors.initials: A
authors.lastname: Williams
authors.forename: Jian-Guo
authors.initials: JG
authors.lastname: Qu
authors.forename: D Mark
authors.initials: DM
authors.lastname: Estes
mesh_terms: D017503:Adenovirus E3 Proteins / Q000235:genetics / Q000378:metabolism*; D000260:Adenoviruses, Human / Q000235:genetics / Q000254:growth & development*; D002460:Cell Line; D006801:Humans; D010957:Plasmids; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D014162:Transfection; D019562:Viral Load; D010948:Viral Plaque Assay
chemical_list: D017503:Adenovirus E3 Proteins; D011994:Recombinant Proteins; C099609:adenovirus death protein, Adenovirus
keywords: Adenovirus death protein; Gene transfer vector; Human adenovirus type 41; Lytic replication