title: An abnormal phenotype of lung Vγ9Vδ2 T cells impairs their responsiveness in tuberculosis patients.
abstract: Antigen-specific γδ T cells represent an early innate defense known to play an important role in anti-mycobacterial immunity. We have investigated the immune functions of Vγ9Vδ2 T cells from Broncho-Alveolar lavages (BAC) samples of active TB patients. We observed that BAC Vγ9Vδ2 T cells presented a strong down-modulation of CD3 expression compared with Vγ9Vδ2 T cells from peripheral blood. Furthermore, Vγ9Vδ2 T cells mainly showed a central memory phenotype, expressed high levels of NK inhibitory receptors and TEMRA cells showed low expression of CD16 compared to circulating Vγ9Vδ2 T cells. Interestingly, the ability of BAC Vγ9Vδ2 T cells to respond to antigen stimulation was dramatically reduced, differently from blood counterpart. These observations indicate that γδ T cell functions are specifically impaired in situ by active TB, suggesting that the alveolar ambient during tuberculosis may affect resident γδ T cells in comparison to circulating cells.
authors.affiliation: Laboratory of Cellular Immunology, National Institute for Infectious Diseases Lazzaro Spallanzani, Rome, Italy. saryeldaker@yahoo.it
authors.forename: Sary
authors.initials: S
authors.lastname: El Daker
authors.forename: Alessandra
authors.initials: A
authors.lastname: Sacchi
authors.forename: Carla
authors.initials: C
authors.lastname: Montesano
authors.forename: Alfonso Maria
authors.initials: AM
authors.lastname: Altieri
authors.forename: Giovanni
authors.initials: G
authors.lastname: Galluccio
authors.forename: Vittorio
authors.initials: V
authors.lastname: Colizzi
authors.forename: Federico
authors.initials: F
authors.lastname: Martini
authors.forename: Angelo
authors.initials: A
authors.lastname: Martino
mesh_terms: D000293:Adolescent; D000328:Adult; D001992:Bronchoalveolar Lavage Fluid / Q000276:immunology; D017252:CD3 Complex / Q000276:immunology / Q000378:metabolism; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007156:Immunologic Memory / Q000276:immunology; D016130:Immunophenotyping; D007371:Interferon-gamma / Q000276:immunology / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000276:immunology / Q000378:metabolism; D008168:Lung / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D008297:Male; D008875:Middle Aged; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000276:immunology* / Q000378:metabolism; D017452:Receptors, IgG / Q000276:immunology / Q000378:metabolism; D055607:Receptors, Natural Killer Cell / Q000276:immunology / Q000378:metabolism; D013601:T-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D014376:Tuberculosis / Q000097:blood / Q000276:immunology* / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology / Q000378:metabolism; D055815:Young Adult
chemical_list: D017252:CD3 Complex; D016692:Receptors, Antigen, T-Cell, gamma-delta; D017452:Receptors, IgG; D055607:Receptors, Natural Killer Cell; D014409:Tumor Necrosis Factor-alpha; D007371:Interferon-gamma
keywords: Lung; Mycobacterium; Primary infection; γδ T cells