title: Destabilization of the oxygen evolving complex of photosystem II by Al3+.
abstract: The inhibitory effect of Al(3+) on photosynthetic electron transport was investigated in isolated thylakoid membranes of spinach. A combination of oxygen evolution, chlorophyll fluorescence induction (FI) and decay and thermoluminescence measurements have been used to characterize photosystem II (PSII) electron transport in the presence of this toxic metal cation. Our results show that below 3 mm, Al(3+) already caused a destabilization of the Mn4 O5 Ca cluster of the oxygen evolving complex (OEC). At these concentrations, an increase in the relative amplitude of the first phase (OJ) of FI curve and retardation of the fluorescence decay kinetics following excitation with a single turnover flash were also observed. A transmembrane structural modification of PSII polypeptides due to the interaction of Al(3+) at the OEC is proposed to retard electron transfer between the quinones QA and QB . Above 3 mm, Al(3+) strongly retarded fluorescence induction and significantly reduced Fv /Fm together with the maximal amplitude of chlorophyll fluorescence induced by a single turnover flash. This chlorophyll fluorescence quenching was attributed to the formation of P680(+) due to inhibition of electron transfer between tyrosine 161 of D1 subunit and P680.
authors.affiliation: Groupe de Recherche en Biologie Végétale (GRBV), Département de chimie, biochimie et physique, Université du Québec à Trois-Rivières, Trois-Rivières, QC, Canada.
authors.forename: Imed
authors.initials: I
authors.lastname: Hasni
authors.forename: Saber
authors.initials: S
authors.lastname: Hamdani
authors.forename: Robert
authors.initials: R
authors.lastname: Carpentier
mesh_terms: D000535:Aluminum / Q000737:chemistry*; D004579:Electron Transport; D005453:Fluorescence; D049449:Luminescence; D010100:Oxygen / Q000737:chemistry*; D045332:Photosystem II Protein Complex / Q000737:chemistry*
chemical_list: D045332:Photosystem II Protein Complex; D000535:Aluminum; D010100:Oxygen