title: Spectrofluorimetric estimation of salbutamol sulphate in different dosage forms by formation of inclusion complex with β-cyclodextrin.
abstract: A simple, precise, reproducible and accurate spectrofluorimetric method for estimation of Salbutamol sulphate (SAL) in bulk drug and various dosage forms has been developed. This method is based on formation of inclusion complex of SAL in β-cyclodextrin (BCD) which gives fluorescence at excitation wavelength of 279.6 nm and emission wavelength of 609.8 nm in water. Formation of inclusion complex of drug with BCD enhances fluorescence intensity of drug leads to increased sensitivity. The developed method was validated according to ICH guidelines with respect to accuracy, precision, linearity, limit of detection, limit of quantification. Linearity was observed in the range of 4-20 μg/ml with correlation coefficient of 0.9982. The simplicity of the method permitted rapid analysis suitable for routine control. The developed method was successfully applied for the estimation of SAL in different marketed dosage forms like tablets, syrup and aerosol.
authors.affiliation: Department of Pharmaceutical Analysis, Institute of Pharmacy, Nirma University, Sarkhej-Gandhinagar Highway, Ahmadabad - 382 481 Gujarat, India.
authors.forename: Harshit Narmadashankar
authors.initials: HN
authors.lastname: Pandya
authors.forename: Hiren Harshadlal
authors.initials: HH
authors.lastname: Berawala
authors.forename: Deepak Mohanlal
authors.initials: DM
authors.lastname: Khatri
authors.forename: Priti Jignesh
authors.initials: PJ
authors.lastname: Mehta
keywords: Salbutamol sulphate; method validation pharmaceutical formulations; spectrofluorometry; β-cyclodextrin