title: Differentiation of mesenchymal stem cells from human umbilical cord tissue into odontoblast-like cells using the conditioned medium of tooth germ cells in vitro.
abstract: The easily accessible mesenchymal stem cells in the Wharton's jelly of human umbilical cord tissue (hUCMSCs) have excellent proliferation and differentiation potential, but it remains unclear whether hUCMSCs can differentiate into odontoblasts. In this study, mesenchymal stem cells were isolated from the Wharton's jelly of human umbilical cord tissue using the simple method of tissue blocks culture attachment. UCMSC surface marker expression was then evaluated for the isolated cells using flow cytometry. The third-passage hUCMSCs induced by conditioned medium from developing tooth germ cells (TGC-CM) displayed high alkaline phosphatase (ALP) levels (P < 0.001), an enhanced ability to proliferate (P < 0.05), and the presence of mineralized nodules. These effects were not observed in cells treated with regular medium. After induction of hUCMSCs, the results of reverse transcriptional polymerase chain reaction (PCR) indicated that the dentin sialophosphoprotein (DSPP) and dentin matrix protein 1 (DMP1) genes were significantly tested. Additionally, dentin sialoprotein (DSP) and DMP1 demonstrated significant levels of staining in an immunofluorescence analysis. In contrast, the control cells failed to display the characteristics of odontoblasts. Taken together, these results suggest that hUCMSCs can be induced to differentiate into odontoblast-like cells with TGC-CM and provide a novel strategy for tooth regeneration research.
authors.affiliation: Department of Prosthodontics, The First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150001, China.
authors.forename: Tian Xia
authors.initials: TX
authors.lastname: Li
authors.forename: Jie
authors.initials: J
authors.lastname: Yuan
authors.forename: Yan
authors.initials: Y
authors.lastname: Chen
authors.forename: Li Jie
authors.initials: LJ
authors.lastname: Pan
authors.forename: Chun
authors.initials: C
authors.lastname: Song
authors.forename: Liang Jia
authors.initials: LJ
authors.lastname: Bi
authors.forename: Xiao Hui
authors.initials: XH
authors.lastname: Jiao
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D002113:Calcification, Physiologic / Q000187:drug effects; D002448:Cell Adhesion / Q000187:drug effects; D002453:Cell Cycle / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects*; D002469:Cell Separation; D048430:Cell Shape / Q000187:drug effects; D017077:Culture Media, Conditioned / Q000494:pharmacology*; D006801:Humans; D007231:Infant, Newborn; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000201:enzymology; D009804:Odontoblasts / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D009805:Odontogenesis / Q000187:drug effects; D014083:Tooth Germ / Q000166:cytology*; D014470:Umbilical Cord / Q000166:cytology*
chemical_list: D017077:Culture Media, Conditioned; D000469:Alkaline Phosphatase