title: [Immunohistochemistry using epidermal growth factor receptor mutation-specific antibodies of delE746-A750 and L858R in lung adenocarcinomas].
abstract: OBJECTIVE
  To evaluate the immunohistochemical detection of epidermal growth factor receptor(EGFR) mutations using two EGFR mutation-specific monoclonal antibodies: delE746-A750 and L858R.
  
  
  METHODS
  A total of 175 paraffin-embedded lung adenocarcinoma tissue samples previously genotyped by directive DNA sequencing were subject to immunostaining using delE746-A750 and L858R antibodies.
  
  
  RESULTS
  There was no significant difference of mutation detection between DNA sequence analysis and delE746-A750 and/or L858R immunostaining (33.7% vs 30.9%, P > 0.05). The overall sensitivity, specificity, positive predictive value and negative predictive value of immunostaining using these two EGFR mutation-specific antibodies were 83.1%, 95.7%, 90.7% and 90.9%, respectively.
  
  
  CONCLUSIONS
  With high sensitivity and good specificity, immunohistochemistry using EGFR mutation-specific monoclonal antibodies is an adequate, easy and cost-effective prescreening method to detect EGFR mutations using paraffin-embedded tissue specimens of lung adenocarcinomas.
authors.affiliation: Department of Pathology, Nanjing University Medical School, Nanjing, China.
authors.forename: Xiang-shan
authors.initials: XS
authors.lastname: Fan
authors.forename: Biao
authors.initials: B
authors.lastname: Liu
authors.forename: Bo
authors.initials: B
authors.lastname: Yu
authors.forename: Shan-shan
authors.initials: SS
authors.lastname: Shi
authors.forename: Xuan
authors.initials: X
authors.lastname: Wang
authors.forename: Jin
authors.initials: J
authors.lastname: Zhang
authors.forename: Jian-dong
authors.initials: JD
authors.lastname: Wang
authors.forename: Zhen-feng
authors.initials: ZF
authors.lastname: Lu
authors.forename: Heng-hui
authors.initials: HH
authors.lastname: Ma
authors.forename: Xiao-jun
authors.initials: XJ
authors.lastname: Zhou
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D000077192:Adenocarcinoma of Lung; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000911:Antibodies, Monoclonal; D004252:DNA Mutational Analysis; D066246:ErbB Receptors / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D005260:Female; D017353:Gene Deletion; D006801:Humans; D007150:Immunohistochemistry; D008175:Lung Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D008297:Male; D008875:Middle Aged; D016612:Paraffin Embedding; D017354:Point Mutation; D012680:Sensitivity and Specificity
chemical_list: D000911:Antibodies, Monoclonal; C512478:EGFR protein, human; D066246:ErbB Receptors