title: Characterization of AntB, a promiscuous acyltransferase involved in antimycin biosynthesis.
abstract: The in vivo and in vitro characterization of AntB, a dedicated acyltransferase encoded in the antimycin biosynthetic gene cluster, which catalyzes the C-8 acyloxy formation is reported. It is demonstrated that AntB has broad substrate specificity toward both the acyl substrate and the acyl carrier and produces more antimycin analogues with varying C-8 acyloxy moieties.
authors.affiliation: Department of Chemical and Biomolecular Engineering and Energy Biosciences Institute, University of California, Berkeley, California 94720, USA.
authors.forename: Moriah
authors.initials: M
authors.lastname: Sandy
authors.forename: Xuejun
authors.initials: X
authors.lastname: Zhu
authors.forename: Zhe
authors.initials: Z
authors.lastname: Rui
authors.forename: Wenjun
authors.initials: W
authors.lastname: Zhang
mesh_terms: D000217:Acyltransferases / Q000737:chemistry* / Q000378:metabolism*; D000968:Antimycin A / Q000031:analogs & derivatives* / Q000096:biosynthesis / Q000737:chemistry; D002384:Catalysis; D004926:Escherichia coli / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D005810:Multigene Family; D013379:Substrate Specificity
chemical_list: C032456:antimycin; D000968:Antimycin A; D000217:Acyltransferases