title: Molecular epidemiology of Escherichia coli O25b-ST131 isolates causing community-acquired UTIs in Mexico.
abstract: Escherichia coli is a common uropathogen causing community-acquired urinary tract infections (UTIs). Out of 4735 E. coli community-acquired UTIs, 10.2% were extended spectrum β-lactamases (ESBL)-producing. The identified ESBL types were CTX-M-15 (96.4%), SHV-2a (3%), and TLA-1 (1%). Of the isolates, 94.6% tested positive for plasmid-mediated quinolone resistance (PMQR) genes (aac(6')-lb-cr [92.1%] and qepA1 [7%] and for qnr-determinants [3.5%]). E. coli O25b-ST131 was identified in 25% of the isolates that harbor a non-conjugative 160-kb plasmid (IncFIA) containing the CTX-M-15, and all of these isolates were found to contain PMQR genes. This work can be useful in modeling the potential impact that may have on community-acquired UTIs in Mexico.
authors.affiliation: Departamento de Diagnóstico Epidemiológico, Centro de Investigaciones Sobre Enfermedades Infecciosas, Instituto Nacional de Salud Pública, Cuernavaca, Morelos, México.
authors.forename: Fernando
authors.initials: F
authors.lastname: Reyna-Flores
authors.forename: Humberto
authors.initials: H
authors.lastname: Barrios
authors.forename: Ulises
authors.initials: U
authors.lastname: Garza-Ramos
authors.forename: Alejandro
authors.initials: A
authors.lastname: Sánchez-Pérez
authors.forename: Teresa
authors.initials: T
authors.lastname: Rojas-Moreno
authors.forename: Felipe J
authors.initials: FJ
authors.lastname: Uribe-Salas
authors.forename: Reynero
authors.initials: R
authors.lastname: Fagundo-Sierra
authors.forename: Jesus
authors.initials: J
authors.lastname: Silva-Sanchez
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002648:Child; D002675:Child, Preschool; D017714:Community-Acquired Infections / Q000453:epidemiology / Q000382:microbiology; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics* / Q000302:isolation & purification; D004927:Escherichia coli Infections / Q000453:epidemiology / Q000382:microbiology*; D005260:Female; D006801:Humans; D008297:Male; D008800:Mexico / Q000453:epidemiology; D008826:Microbial Sensitivity Tests; D008875:Middle Aged; D017720:Molecular Epidemiology; D058889:Molecular Typing; D010957:Plasmids / Q000235:genetics; D015363:Quinolones / Q000494:pharmacology; D014552:Urinary Tract Infections / Q000453:epidemiology / Q000382:microbiology*; D055815:Young Adult; D018440:beta-Lactam Resistance / Q000235:genetics; D001618:beta-Lactamases / Q000235:genetics
chemical_list: D000900:Anti-Bacterial Agents; D015363:Quinolones; C473109:beta-lactamase CTX-M-15; D001618:beta-Lactamases