title: Bacillus songklensis sp. nov., isolated from soil.
abstract: A Gram-stain-positive, spore-forming, rod-shaped, motile, strictly aerobic bacterial strain, designated CAU 1033(T), was isolated from soil and its taxonomic position was investigated using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain CAU 1033(T) formed a distinct lineage within the genus Bacillus and was most closely related to Bacillus drentensis KCTC 13025(T) (similarity 95.9 %). CAU 1033(T) contained MK-7 as the only isoprenoid quinone and iso-C15 : 0 and anteiso-C15 : 0 as the major fatty acids. The cell wall peptidoglycan of strain CAU 1033(T) contained meso-diaminopimelic acid and the major whole-cell sugars were arabinose, sucrose and ribose. The polar lipids were composed of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, two unidentified phospholipids, four unidentified aminophospholipids, an unidentified aminolipid, two unidentified glycolipids and another unidentified polar lipid. The DNA G+C content was 41.4 mol%. On the basis of phenotypic data and phylogenetic inference, strain CAU 1033(T) was classified as a representative of a novel species in the genus Bacillus for which the name Bacillus songklensis sp. nov. is proposed. The type strain is CAU 1033(T) ( = KCTC 13881(T) = CCUG 61889(T)).
authors.affiliation: Department of Microbiology, Chung-Ang University College of Medicine, Seoul, Republic of Korea.
authors.forename: Hyeonji
authors.initials: H
authors.lastname: Kang
authors.affiliation: Department of Microbiology, Chung-Ang University College of Medicine, Seoul, Republic of Korea.
authors.forename: Veeraya
authors.initials: V
authors.lastname: Weerawongwiwat
authors.affiliation: Department of Microbiology, Chung-Ang University College of Medicine, Seoul, Republic of Korea.
authors.forename: Jong-Hwa
authors.initials: JH
authors.lastname: Kim
authors.affiliation: Department of Microbiology, Faculty of Science, Prince of Songkla University, Songkhla 90112, Thailand.
authors.forename: Ampaitip
authors.initials: A
authors.lastname: Sukhoom
authors.affiliation: Department of Microbiology, Chung-Ang University College of Medicine, Seoul, Republic of Korea.
authors.forename: Wonyong
authors.initials: W
authors.lastname: Kim
mesh_terms: D001407:Bacillus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002241:Carbohydrates / Q000737:chemistry; D004269:DNA, Bacterial / Q000235:genetics; D003960:Diaminopimelic Acid / Q000737:chemistry; D005227:Fatty Acids / Q000737:chemistry; D008969:Molecular Sequence Data; D010457:Peptidoglycan / Q000737:chemistry; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D013785:Thailand; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D002241:Carbohydrates; D004269:DNA, Bacterial; D005227:Fatty Acids; D010457:Peptidoglycan; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; D003960:Diaminopimelic Acid; C062629:menaquinone 7