title: Experimental study on the behavior of primary human osteoblasts on laser-cused pure titanium surfaces.
abstract: Mandibular tumor resection can lead to a mandibular segmental defect. LaserCUSING® is used to produce a mandibular implant, designed to be identical to the shape of the mandibular defect. Novel microrough surfaces result from this generative technology. In the current study, the behavior of human osteoblasts on untreated laser-cused titanium specimens or on specimens conditioned with different blasting agents was analyzed. The conditioning of these specimens resulted in surfaces with graded roughness. White light confocal microscopy and single-cell force spectroscopy were used to characterize the surface of the specimens and to quantify the initial adhesion of primary human osteoblasts to the specimens, respectively. Furthermore, cell growth, viability, apoptosis as well as mineralization of the specimens were analyzed over a time-period of 2 months. Compared to specimens that were treated with blasting agents, untreated specimens had the highest surface roughness. Quantitative SCFS measurements demonstrated that the adhesion of human primary osteoblasts was the highest on these specimens. Additionally, the untreated specimens allowed the highest number of osteoblasts to colonize. Mineralization studies showed increasing calcium and phosphor elemental composition for all specimen series. It can be concluded that untreated laser-cused titanium specimens are superior to promote the initial adhesion and subsequent colonization by osteoblast cells.
authors.affiliation: Department of Oral and Maxillofacial Surgery, University Hospital, Technische Universität Dresden, Fetscherstraße 74, Dresden, Germany.
authors.forename: Jutta
authors.initials: J
authors.lastname: Markwardt
authors.forename: Jens
authors.initials: J
authors.lastname: Friedrichs
authors.forename: Carsten
authors.initials: C
authors.lastname: Werner
authors.forename: Andreas
authors.initials: A
authors.lastname: Davids
authors.forename: Hartmut
authors.initials: H
authors.lastname: Weise
authors.forename: Raoul
authors.initials: R
authors.lastname: Lesche
authors.forename: Anke
authors.initials: A
authors.lastname: Weber
authors.forename: Ursula
authors.initials: U
authors.lastname: Range
authors.forename: Heike
authors.initials: H
authors.lastname: Meißner
authors.forename: Günther
authors.initials: G
authors.lastname: Lauer
authors.forename: Bernd
authors.initials: B
authors.lastname: Reitemeier
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D002113:Calcification, Physiologic / Q000187:drug effects; D002448:Cell Adhesion / Q000187:drug effects; D002452:Cell Count; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D006801:Humans; D007834:Lasers; D018613:Microscopy, Confocal; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D013057:Spectrum Analysis; D013499:Surface Properties; D014025:Titanium / Q000494:pharmacology*
chemical_list: D014025:Titanium
keywords: SCFS; laser-cused pure titanium; long-term cell behavior; osteoblasts; surface topography