title: Longitudinal and integrative biomodeling of effector and memory immune compartments after inactivated influenza vaccination.
abstract: Most vaccines, including those against influenza, were developed by focusing solely on humoral response for protection. However, vaccination activates different adaptive compartments that might play a role in protection. We took advantage of the pandemic 2009 A(H1N1) influenza vaccination to conduct a longitudinal integrative multiparametric analysis of seven immune parameters in vaccinated subjects. A global analysis underlined the predominance of induction of humoral and CD4 T cell responses, whereas pandemic 2009 A(H1N1)-specific CD8 responses did not improve after vaccination. A principal component analysis and hierarchical clustering of individuals showed a differential upregulation of influenza vaccine-specific immunity including hemagglutination inhibition titers, IgA(+) and IgG(+) Ab-secreting cells, effector CD4 or CD8 T cell frequencies at day 21 among individuals, suggesting a fine-tuning of the immune parameters after vaccination. This is related to individual factors including the magnitude and quality of influenza-specific immune responses before vaccination. We propose a graphical delineation of immune determinants that would be essential for a better understanding of vaccine-induced immunity in vaccination strategies.
authors.affiliation: INSERM Unité Mixte de Recherche S-945, Laboratory of Immunity and Infection, University of Pierre and Marie Curie, Paris 06, 75013 Paris, France.
authors.forename: Olivia
authors.initials: O
authors.lastname: Bonduelle
authors.forename: Nora
authors.initials: N
authors.lastname: Yahia
authors.forename: Sophie
authors.initials: S
authors.lastname: Siberil
authors.forename: Nora
authors.initials: N
authors.lastname: Benhabiles
authors.forename: Fabrice
authors.initials: F
authors.lastname: Carrat
authors.forename: Anne
authors.initials: A
authors.lastname: Krivine
authors.forename: Flore
authors.initials: F
authors.lastname: Rozenberg
authors.forename: Jordan
authors.initials: J
authors.lastname: Dimitrov
authors.forename: Srini V
authors.initials: SV
authors.lastname: Kaveri
authors.forename: Angélique
authors.initials: A
authors.lastname: Curjol
authors.forename: Malka
authors.initials: M
authors.lastname: Tindel
authors.forename: Martine
authors.initials: M
authors.lastname: Louet
authors.forename: Florent
authors.initials: F
authors.lastname: Desert
authors.forename: Odile
authors.initials: O
authors.lastname: Launay
authors.forename: Pierre
authors.initials: P
authors.lastname: Loulergue
authors.forename: Gwenaelle
authors.initials: G
authors.lastname: Badre
authors.forename: Christine
authors.initials: C
authors.lastname: Katlama
authors.forename: François
authors.initials: F
authors.lastname: Bricaire
authors.forename: Assia
authors.initials: A
authors.lastname: Samri
authors.forename: Dominique
authors.initials: D
authors.lastname: Rousset
authors.forename: Sylvie
authors.initials: S
authors.lastname: van der Werf
authors.forename: Stephane
authors.initials: S
authors.lastname: Jauréguiberry
authors.forename: Behazine
authors.initials: B
authors.lastname: Combadiere
mesh_terms: D000914:Antibodies, Viral / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D006385:Hemagglutination Inhibition Tests; D006801:Humans; D007070:Immunoglobulin A / Q000276:immunology; D007074:Immunoglobulin G / Q000276:immunology; D007156:Immunologic Memory; D053118:Influenza A Virus, H1N1 Subtype / Q000276:immunology*; D007252:Influenza Vaccines / Q000008:administration & dosage / Q000276:immunology*; D007251:Influenza, Human / Q000276:immunology* / Q000517:prevention & control; D025341:Principal Component Analysis; D014611:Vaccination; D015164:Vaccines, Inactivated / Q000008:administration & dosage / Q000276:immunology
chemical_list: D000914:Antibodies, Viral; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007252:Influenza Vaccines; D015164:Vaccines, Inactivated