title: Effects of M II stage oocytes zona pellucida birefringence on pregnancy outcome.
abstract: OBJECTIVE
  To explore the effects of different M II stage oocytes zona pellucida birefringence on pregnancy outcome.
  
  
  METHODS
  A total of 46 couples with infertile which induced by single cause received in-vitro fertilization treatment were analyzed retrospectively, and randomly divided into the high zona birefringence (HZB)/HZB group, HZB/low zona birefringence (LZB) group and LZB/LZB group according to different oocytes zona pellucida birefringence. Intracytoplasmic sperm injection outcome was analyzed and compared.
  
  
  RESULTS
  The proportion of HZB oocytes, implantation rate and the pregnancy rate were decreased in three groups (HZB/HZB group>HZB/LZB group>LZB/LZB group) (P<0.05). But there was no significantly different between the number of oocytes and fertilization rate of these groups (P>0.05). Logistic regression analysis showed that factors affect M II stage oocytes zona pellucida birefringence were age, basal FSH level and the LH level on the day of HCG injection. Age and FSH levels were negatively correlated with the single oocyte zona pellucida birefringence; While the LH level on the day of hCG injection was positively correlated with the single oocyte zona pellucida birefringence.
  
  
  CONCLUSIONS
  The primary influence factors on M II stage oocytes zona pellucida are age, basal FSH level and the LH level on the day of hCG injection. The birefringence value of zona pellucida can affect the pregnancy outcome.
authors.affiliation: Reproductive Center of the First Affiliated Hospital of Sun Yat-sen University, Number 58, Zhongshan Road, Guangzhou 510080, China.
authors.forename: Jia
authors.initials: J
authors.lastname: Luo
authors.forename: Yan-Wen
authors.initials: YW
authors.lastname: Xu
authors.forename: Ming-Fang
authors.initials: MF
authors.lastname: Zhang
authors.forename: Ling
authors.initials: L
authors.lastname: Gao
authors.forename: Cong
authors.initials: C
authors.lastname: Fang
authors.forename: Can-Quan
authors.initials: CQ
authors.lastname: Zhou
mesh_terms: D000328:Adult; D000367:Age Factors; D001718:Birefringence; D010064:Embryo Implantation / Q000502:physiology*; D005260:Female; D005640:Follicle Stimulating Hormone / Q000378:metabolism; D006801:Humans; D007248:Infertility, Male / Q000628:therapy*; D016015:Logistic Models; D007986:Luteinizing Hormone / Q000378:metabolism; D008297:Male; D009865:Oocytes / Q000502:physiology*; D011247:Pregnancy; D011256:Pregnancy Outcome; D011994:Recombinant Proteins; D012189:Retrospective Studies; D020554:Sperm Injections, Intracytoplasmic; D015044:Zona Pellucida / Q000502:physiology*
chemical_list: D011994:Recombinant Proteins; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone