title: Rapid separations by LC using ion-exchange media based on spongy monoliths.
abstract: Novel sponge-based monoliths containing ionic functional groups were developed for rapid separation and/or concentration of ionic solutes. The cationic and anionic spongy monoliths were prepared by chemical modifications of the pore surface on an original spongy monolith consisting of poly(ethylene-co-vinyl acetate). After hydrolysis of the spongy monolith, an anionic or a cationic moiety was introduced with succinyl chloride or acryloyl chloride/diethylamine, respectively. As a result of liquid chromatographic evaluations for the columns packed with these ionic spongy monoliths, both anionic and cationic monoliths showed ionic interactions with the opposing ionic solutes even if a higher flow rate (9.0 mL/min) was employed. Furthermore, we demonstrated the effective and rapid preconcentration of adenosine 5'-monophosphate in water using column-switching LC combined with the cationic spongy monolith as an online SPE adsorbent.
authors.affiliation: Graduate School of Engineering, Kyoto University, Katsura, Nishikyo-ku, Kyoto, Japan. kubo@anchem.mc.kyoto-u.ac.jp
authors.forename: Takuya
authors.initials: T
authors.lastname: Kubo
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Tanigawa
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Tominaga
authors.forename: Ken
authors.initials: K
authors.lastname: Hosoya
authors.forename: Koji
authors.initials: K
authors.lastname: Otsuka
keywords: Chemical modification; Ionic solutes; Online preconcentration; Rapid separation; Spongy monoliths