title: [The estimate of association between gene polymorphisms and the frequency and spectrum of cytogenetic abnormalities in the cohort of Siberian Group of Chemical Enterprises employees exposed to professional irradiation (microarray studies)].
abstract: The results from the research into the association between polymorphisms of genes-candidates for individual radiosensitivity and the frequency and spectrum of cytogenetic abnormalities are analyzed. The study was conducted among Siberian Group of Chemical Enterprises healthy employees exposed to professional irradiation in a dose range of 100-300 mSv. Genotyping of DNA samples from 96 employees was carried out by oligonucleotide microarray: "Cancer_SNP_Panel GT-17-211" ("Illumina") containing 1, 421 SNP-markers (Single Nucleotide Polymorphisms) of 406 genes. The standard cytogenetic analysis was performed in the entire examined group. We have also analyzed the association of these SNP with the frequencies of aberrant cells and following chromosomal aberrations: single chromatid fragments, chromatid exchanges, paired fragments, dicentric, ring and frequencies, translocations. We have found that 40 SNP (rs1800389, rs1051690, rs2392221, rs1041163, rs2114443, rs6083, rs1760904, rs4986894, rs488133, rs7462102, rs11249938, rs34206126, rs33945943, rs34324628, rs5742694, rs978458, rs5742667, rs2373721, rs2162679, rs889162, rs2233679, rs2010457, rs2873950, rs1574154, rs10934500, rs4688046, rs10934503, rs4624596, rs2288729, rs4227, rs1367696, rs751087, rs1269486, rs1149901, rs1800404, rs887477, rs696405, rs751087, rs81 92284, rs312016) are associated with the frequency of different types of chromosomal abnormalities (p-value with FDR of Benjamini-Hochberg--equal less than 0.05). 24 SNP (underlined) are associated with more than one type of chromosome abnormalities. In the future, we are going to confirm the results in further studies on the cohort of more than 600 persons.
authors.forename: N V
authors.initials: NV
authors.lastname: Litviakov
authors.forename: O O
authors.initials: OO
authors.lastname: Goncharik
authors.forename: M B
authors.initials: MB
authors.lastname: Freĭdin
authors.forename: A E
authors.initials: AE
authors.lastname: Sazonov
authors.forename: E O
authors.initials: EO
authors.lastname: Vasil'eva
authors.forename: S A
authors.initials: SA
authors.lastname: Mezheritskiĭ
authors.forename: M V
authors.initials: MV
authors.lastname: Khaliuzova
authors.forename: A A
authors.initials: AA
authors.lastname: Bondariuk
authors.forename: E N
authors.initials: EN
authors.lastname: Al'bakh
authors.forename: A B
authors.initials: AB
authors.lastname: Karpov
authors.forename: R M
authors.initials: RM
authors.lastname: Takhauov
mesh_terms: D000368:Aged; D002869:Chromosome Aberrations / Q000528:radiation effects*; D056726:Genetic Association Studies; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D016273:Occupational Exposure; D020641:Polymorphism, Single Nucleotide; D011836:Radiation Tolerance / Q000235:genetics*; D011839:Radiation, Ionizing; D012800:Siberia; D012854:Sister Chromatid Exchange / Q000528:radiation effects*