title: [An experimental model of mass-type brain damage in the rat: expression of brain damage based on neurospecific enolase and protein S100B].
abstract: OBJECTIVE
  To determine whether a model of transient mass-type brain damage (MTBD) in the rat produces early release of neurospecific enolase (NSE) and protein S100B in peripheral blood, as an expression of the induced brain injury.
  
  
  METHODS
  An experimental study with a control group.
  
  
  METHODS
  Experimental operating room of the Institute of Biomedicine (IBiS) of Virgen del Rocío University Hospital (Seville, Spain).
  
  
  METHODS
  Fourteen adult Wistar rats.
  
  
  METHODS
  Blood was sampled at baseline, followed by: MTBD group, a trephine perforation was used to insert and inflate the balloon of a catheter at a rate of 500 μl/20 sec, followed by 4 blood extractions every 20 min. Control group, the same procedure as before was carried out, though without trephine perforation.
  Weight, early mortality, serum NSE and S100B concentration.
  
  
  RESULTS
  Differences in NSE and S100B concentration were observed over time within the MTBD group (P<.001), though not so in the control group. With the exception of the baseline determination, differences were observed between the two groups in terms of the mean NSE and S100B values. Following MTBD, NSE and S100B progressively increased at all measurement timepoints, with r=0.765; P=.001 and r=0.628; P=.001, respectively. In contrast, the control group showed no such correlation for either biomarker.
  
  
  CONCLUSIONS
  Serum NSE and S100B concentrations offer an early indication of brain injury affecting the gray and white matter in an experimental model of mass-type MTBD in the rat.
authors.affiliation: Unidad de Neurocríticos, H.U. Virgen del Rocío, IBiS/CSIC Universidad de Sevilla, Sevilla, España. Electronic address: juanjoegea@hotmail.com.
authors.forename: J J
authors.initials: JJ
authors.lastname: Egea-Guerrero
authors.affiliation: Unidad de Neurocríticos, H.U. Virgen del Rocío, IBiS/CSIC Universidad de Sevilla, Sevilla, España.
authors.forename: F
authors.initials: F
authors.lastname: Murillo-Cabezas
authors.affiliation: Departamento de Bioquímica Clínica, H.U. Virgen del Rocío, IBiS/CSIC Universidad de Sevilla, Sevilla, España.
authors.forename: A
authors.initials: A
authors.lastname: Rodríguez-Rodríguez
authors.affiliation: Unidad de Neurocríticos, H.U. Virgen del Rocío, IBiS/CSIC Universidad de Sevilla, Sevilla, España.
authors.forename: E
authors.initials: E
authors.lastname: Gordillo-Escobar
authors.affiliation: Unidad de Neurocríticos, H.U. Virgen del Rocío, IBiS/CSIC Universidad de Sevilla, Sevilla, España.
authors.forename: J
authors.initials: J
authors.lastname: Revuelto-Rey
authors.affiliation: Unidad de Neurocríticos, H.U. Virgen del Rocío, IBiS/CSIC Universidad de Sevilla, Sevilla, España.
authors.forename: M A
authors.initials: MA
authors.lastname: Muñoz-Sánchez
authors.affiliation: Departamento de Bioquímica Clínica, H.U. Virgen del Rocío, IBiS/CSIC Universidad de Sevilla, Sevilla, España.
authors.forename: A
authors.initials: A
authors.lastname: León-Justel
authors.affiliation: Departamento de Medicina Preventiva y Salud Pública, Universidad de Sevilla, IBiS/CSIC Universidad de Sevilla, Sevilla, España.
authors.forename: A
authors.initials: A
authors.lastname: Vilches-Arenas
mesh_terms: D000818:Animals; D001930:Brain Injuries / Q000097:blood*; D004195:Disease Models, Animal; D008297:Male; D010751:Phosphopyruvate Hydratase / Q000097:blood*; D051381:Rats; D017208:Rats, Wistar; D064568:S100 Calcium Binding Protein beta Subunit / Q000097:blood*
chemical_list: D064568:S100 Calcium Binding Protein beta Subunit; D010751:Phosphopyruvate Hydratase
keywords: Enolasa neuroespecífica; Experimental model; Modelo experimental; Neurospecific enolase; Proteína S100B; Rat; Rata; S100B protein; Traumatic brain injury; Traumatismo Craneoencefálico