title: Quantitative and multiplexed microRNA sensing in living cells based on peptide nucleic acid and nano graphene oxide (PANGO).
abstract: MicroRNA (miRNA) is an important small RNA which regulates diverse gene expression at the post-transcriptional level. miRNAs are considered as important biomarkers since abnormal expression of specific miRNAs is associated with many diseases including cancer and diabetes. Therefore, it is important to develop biosensors to quantitatively detect miRNA expression levels. Here, we develop a nanosized graphene oxide (NGO) based miRNA sensor, which allows quantitative monitoring of target miRNA expression levels in living cells. The strategy is based on tight binding of NGO with peptide nucleic acid (PNA) probes, resulting in fluorescence quenching of the dye that is conjugated to the PNA, and subsequent recovery of the fluorescence upon addition of target miRNA. PNA as a probe for miRNA sensing offers many advantages including high sequence specificity, high loading capacity on the NGO surface compared to DNA and resistance against nuclease-mediated degradation. The present miRNA sensor allowed the detection of specific target miRNAs with the detection limit as low as ~1 pM and the simultaneous monitoring of three different miRNAs in a living cell.
authors.affiliation: Department of Chemistry, Seoul National University, Seoul, 151-747, Republic of Korea.
authors.forename: Soo-Ryoon
authors.initials: SR
authors.lastname: Ryoo
authors.forename: Jieon
authors.initials: J
authors.lastname: Lee
authors.forename: Jinah
authors.initials: J
authors.lastname: Yeo
authors.forename: Hee-Kyung
authors.initials: HK
authors.lastname: Na
authors.forename: Young-Kwan
authors.initials: YK
authors.lastname: Kim
authors.forename: Hongje
authors.initials: H
authors.lastname: Jang
authors.forename: Jung Hyun
authors.initials: JH
authors.lastname: Lee
authors.forename: Sang Woo
authors.initials: SW
authors.lastname: Han
authors.forename: Younghoon
authors.initials: Y
authors.lastname: Lee
authors.forename: Vic Narry
authors.initials: VN
authors.lastname: Kim
authors.forename: Dal-Hee
authors.initials: DH
authors.lastname: Min
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006108:Graphite / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D008422:Materials Testing; D035683:MicroRNAs / Q000032:analysis* / Q000737:chemistry / Q000235:genetics*; D008832:Microchemistry / Q000295:instrumentation; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D010087:Oxides / Q000737:chemistry; D010316:Particle Size; D020135:Peptide Nucleic Acids / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000295:instrumentation*; D013499:Surface Properties
chemical_list: D035683:MicroRNAs; D010087:Oxides; D020135:Peptide Nucleic Acids; D006108:Graphite