title: An RP-HPLC-UV method with SPE for cefotaxime in all-in-one total parenteral nutritional admixtures: application to stability studies.
abstract: A simple and selective RP-HPLC-UV method with SPE was developed and validated for the quantification of cefotaxime in all-in-one total parenteral nutrition (AIO-TPN) admixtures. Chromatographic separation was achieved on a 5 pm particle size C18 DB column (250 x 4.6 mm id) using the mobile phase ammonium acetate (25 mM, pH 4.0)-50% acetonitrile in methanol (80 + 20, v/v). The flow rate was 0.9 mL/min and the detection wavelength was 254 nm. The analyte was extracted from AIO-TPN admixtures by means of an SPE method. The cefotaxime calibration curve was linear over a concentration range of 100-1400 microg/mL with a correlation coefficient of > or = 0.9994. The intraday accuracy and precision for cefotaxime were < or = -3.15 and < or = 3.08%, respectively, whereas the interday accuracy and precision were < or = -2.48 and < or = 2.25%, respectively. The method was successfully applied to stability studies of cefotaxime in the presence of micronutrients together with low and high concentrations of macronutrients in AIO-TPN admixtures. Cefotaxime was degraded by 13.00 and 26.05% at room temperature (25 +/- 2 degrees C) after 72 h in low and high macronutrient concentration formulations of AIO-TPN admixtures, respectively. The values of cefotaxime degradation rates for low and high macronutrient concentration formulations of AIO-TPN admixtures were -0.164 and -0.353, respectively. These results indicated that there was a higher rate of degradation in the AIO-TPN admixture formulations containing high concentrations of macronutrients.
authors.affiliation: Universiti Sains Malaysia, School of Pharmaceutical Sciences, 11800, Pulau Pinang, Malaysia. drmmsiqbal@gmail.com
authors.forename: Muhammad Shahid
authors.initials: MS
authors.lastname: Iqbal
authors.forename: Mohd Baidi
authors.initials: MB
authors.lastname: Bahari
authors.forename: Yusrida
authors.initials: Y
authors.lastname: Darwis
authors.forename: Muhammad Zahid
authors.initials: MZ
authors.lastname: Iqbal
authors.forename: Amer
authors.initials: A
authors.lastname: Hayat
authors.forename: Gantala
authors.initials: G
authors.lastname: Venkatesh
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry*; D002110:Caffeine / Q000737:chemistry; D002439:Cefotaxime / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005504:Food Analysis / Q000379:methods / Q000592:standards; D015394:Molecular Structure; D010289:Parenteral Nutrition, Total; D052616:Solid Phase Extraction / Q000379:methods* / Q000592:standards*
chemical_list: D000900:Anti-Bacterial Agents; D002110:Caffeine; D002439:Cefotaxime