title: Detection of intracellular cytokines.
abstract: The intracellular cytokine method allows for a multiparametric readout by flow cytometry with precise phenotyping of the responding T cells. The intracellular cytokine staining of cells that have been fixed and permeabilized following a short-term activation and staining with antibodies to cell surface markers allows for identification of the cellular origin of the cytokine accumulated product.
authors.affiliation: HLA Laboratory, Cedars-Sinai Medical Center, Los Angeles, CA, USA.
authors.forename: Nancy L
authors.initials: NL
authors.lastname: Reinsmoen
authors.forename: Chih-Hung
authors.initials: CH
authors.lastname: Lai
mesh_terms: D000906:Antibodies / Q000276:immunology*; D002462:Cell Membrane / Q000276:immunology; D002463:Cell Membrane Permeability / Q000276:immunology; D016207:Cytokines / Q000276:immunology / Q000302:isolation & purification*; D003593:Cytoplasm / Q000276:immunology; D005434:Flow Cytometry; D006801:Humans; D008213:Lymphocyte Activation; D021381:Protein Transport / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D000906:Antibodies; D016207:Cytokines