title: Spectroscopic study one thiosemicarbazone derivative with ctDNA using ethidium bromide as a fluorescence probe.
abstract: In this study, a thiosemicarbazone derivative (E)-2-((1,4-dihydroxy-9,10-anthraquinone-2-yl)methylene)-N-(4-fluorophenyl)hydrazinecarbothioamide (DAFPT) was synthesized, and the interaction of DAFPT with calf thymus DNA (ctDNA) was explored using ethidium bromide (EB) as a fluorescence probe. The binding mode between DAFPT and ctDNA was investigated by UV absorption spectroscopy, fluorescence spectroscopy and molecular docking. The fluorescence quenching mechanism of EB-ctDNA by DAFPT might be a combined quenching type. Thermodynamic parameters showed that the reaction was spontaneous. According to ionic strength, fluorescence polarization and melting temperature (T(m)) curve results, DAFPT-ctDNA interaction was groove binding. The molecular modeling results indicated that DAFPT could slide into the A-T rich region of ctDNA.
authors.affiliation: School of Chemistry and Chemical Engineering, Henan Normal University, Xinxiang 453007, China.
authors.forename: Shaoguang
authors.initials: S
authors.lastname: Geng
authors.forename: Qing
authors.initials: Q
authors.lastname: Wu
authors.forename: Lei
authors.initials: L
authors.lastname: Shi
authors.forename: Fengling
authors.initials: F
authors.lastname: Cui
mesh_terms: D004247:DNA / Q000737:chemistry*; D004996:Ethidium / Q000737:chemistry*; D008958:Models, Molecular; D008968:Molecular Conformation; D013057:Spectrum Analysis; D013816:Thermodynamics; D013882:Thiosemicarbazones / Q000737:chemistry*
chemical_list: D013882:Thiosemicarbazones; D004247:DNA; C427203:calf thymus DNA; D004996:Ethidium
keywords: (E)-2-((1,4-dihydroxy-9,10-anthraquinone-2-yl) methylene)-N-(4-fluorophenyl) hydrazinecarbothioamide (DAFPT); Calf thymus DNA (ctDNA); Ethidium bromide (EB); Fluorescence spectroscopy; Molecular docking