title: Selective detection of 5-formyl-2'-deoxycytidine in DNA using a fluorogenic hydroxylamine reagent.
abstract: Fluorogenic hydroxylamine reagents were used for detecting 5-fC through a labeling pathway. Chemical synthesis, HPLC, denaturing PAGE, and DNA MS were applied to testify that the probe reacted with 5-fC with oligodeoxynucleotide selectivity to achieve 5-fC detection conveniently and quantificationally with the method of fluorescence. The feasibility of fluorescently detecting 5-fC in a genome was also investigated.
authors.affiliation: College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of Ministry of Education, Wuhan University, Hubei, Wuhan, 430072, P. R. of China.
authors.forename: Pu
authors.initials: P
authors.lastname: Guo
authors.forename: Shengyong
authors.initials: S
authors.lastname: Yan
authors.forename: Jianlin
authors.initials: J
authors.lastname: Hu
authors.forename: Xiwen
authors.initials: X
authors.lastname: Xing
authors.forename: Changcheng
authors.initials: C
authors.lastname: Wang
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Xu
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Qiu
authors.forename: Wen
authors.initials: W
authors.lastname: Ma
authors.forename: Chunjiang
authors.initials: C
authors.lastname: Lu
authors.forename: Xiaocheng
authors.initials: X
authors.lastname: Weng
authors.forename: Xiang
authors.initials: X
authors.lastname: Zhou
mesh_terms: D002851:Chromatography, High Pressure Liquid; D004247:DNA / Q000737:chemistry*; D003841:Deoxycytidine / Q000031:analogs & derivatives* / Q000032:analysis / Q000737:chemistry; D005456:Fluorescent Dyes / Q000032:analysis* / Q000737:chemistry*; D019811:Hydroxylamine / Q000737:chemistry*; D007202:Indicators and Reagents / Q000737:chemistry*; D009838:Oligodeoxyribonucleotides / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
chemical_list: C402104:5-formyl-2'-deoxycytidine; D005456:Fluorescent Dyes; D007202:Indicators and Reagents; D009838:Oligodeoxyribonucleotides; D003841:Deoxycytidine; D019811:Hydroxylamine; D004247:DNA