title: Rhein lysinate decreases the generation of β-amyloid in the brain tissues of Alzheimer's disease model mice by inhibiting inflammatory response and oxidative stress.
abstract: The protective effect of rhein lysinate (RHL) on Alzheimer's disease (AD) was explored in senescence-accelerated mouse prone-8 (SAMP8) mice. SAMP8 mice without treatment were used as the AD-positive control, and senescence-accelerated-resistant mice were used as the AD-negative control. In this study, 4-month-old male SAMP8 mice were orally administered 25 and 50 mg/kg RHL in drinking water for 6 months. The results of brain tissue enzyme-linked immunosorbent assay (ELISA), immunohistochemistry, and Western blot were demonstrated that compared with SAMP8 group, β-amyloid1-40 and β-amyloid1-42 were reduced; the levels of tumor necrosis factor-α and interleukin 6 of brain tissues were also significantly decreased; however, the level of sirtuin 1 (SIRT1) was increased in the RHL-treated group. Compared with SAMP8 group, the ROS levels and malondialdehyde levels were decreased; however, superoxide dismutase and glutathione peroxidase levels were increased in the brain tissues of SAMP8 25 and 50 mg/kg RHL-treated groups. In conclusion, the reduction of Aβ induced by RHL was related to the increase of SIRT1 and the inhibition of the inflammatory response and oxidative stress in SAMP8 mice. It might be a promising biological therapeutic drug for AD.
authors.affiliation: Department of Endocrinology, The Third Hospital of Nanchang City, Nanchang 330009, China.
authors.forename: Jiang
authors.initials: J
authors.lastname: Liu
authors.forename: Gang
authors.initials: G
authors.lastname: Hu
authors.forename: Rong
authors.initials: R
authors.lastname: Xu
authors.forename: Yue
authors.initials: Y
authors.lastname: Qiao
authors.forename: He-Ping
authors.initials: HP
authors.lastname: Wu
authors.forename: Xun
authors.initials: X
authors.lastname: Ding
authors.forename: Peng
authors.initials: P
authors.lastname: Duan
authors.forename: Ping
authors.initials: P
authors.lastname: Tu
authors.forename: Ya-Jun
authors.initials: YJ
authors.lastname: Lin
mesh_terms: D000375:Aging / Q000187:drug effects; D000544:Alzheimer Disease / Q000188:drug therapy*; D000682:Amyloid / Q000494:pharmacology; D016229:Amyloid beta-Peptides / Q000032:analysis / Q000187:drug effects*; D000818:Animals; D000880:Anthraquinones / Q000737:chemistry / Q000494:pharmacology*; D001921:Brain / Q000187:drug effects / Q000378:metabolism; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D005979:Glutathione Peroxidase / Q000032:analysis / Q000378:metabolism; D015850:Interleukin-6 / Q000032:analysis; D008239:Lysine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology*; D008297:Male; D008315:Malondialdehyde / Q000494:pharmacology; D051379:Mice; D018384:Oxidative Stress / Q000187:drug effects; D056564:Sirtuin 1 / Q000032:analysis / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000032:analysis / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
chemical_list: D000682:Amyloid; D016229:Amyloid beta-Peptides; D000880:Anthraquinones; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha; D008315:Malondialdehyde; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; C470545:Sirt1 protein, mouse; D056564:Sirtuin 1; D008239:Lysine; C020491:rhein