title: Bioactivities of Jc-SCRIP, a type 1 ribosome-inactivating protein from Jatropha curcas seed coat.
abstract: In this study, a type 1 RIP, designated as Jc-SCRIP, was first isolated from the seed coat of Jatropha curcas Linn. It was purified by ammonium sulfate precipitation and chromatography on DEAE-Sephacel™ and CM-cellulose columns. Purification fold of Jc-SCRIP increased 113.8 times, and the yield was 1.13% of the total protein in the final step. It was shown to be a monomeric glycoprotein with a molecular mass of 38 938 Da, as determined by MALDI-TOF/MS. It exhibited hemagglutination activity and possessed strong N-glycosidase activity. The antimicrobial activity of Jc-SCRIP was tested against nine human pathogenic bacteria and one fungus; the most potent inhibitory activity was against Staphylococcus epidermidis ATCC 12228, with minimum inhibitory concentration value of 0.20 μm. Jc-SCRIP demonstrated in vitro cytotoxicity against human breast adenocarcinoma cell line (MCF-7), a colon adenocarcinoma (SW620), and a liver carcinoma cell line (HepG2), with IC50 values of 0.15, 0.25, and 0.40 mm, respectively. The results suggested that Jc-SCRIP may be a potential natural antimicrobial and anticancer agent in medical applications.
authors.affiliation: Department of Biochemistry, Faculty of Science, Kasetsart University, 50 Ngamwongwan Rd., Chatujak, Bangkok 10900, Thailand.
authors.forename: Chanthakan
authors.initials: C
authors.lastname: Nuchsuk
authors.forename: Nuanchawee
authors.initials: N
authors.lastname: Wetprasit
authors.forename: Sittiruk
authors.initials: S
authors.lastname: Roytrakul
authors.forename: Kiattawee
authors.initials: K
authors.lastname: Choowongkomon
authors.forename: Nattanan
authors.initials: N
authors.lastname: T-Thienprasert
authors.forename: Chotika
authors.initials: C
authors.lastname: Yokthongwattana
authors.forename: Theerakul
authors.initials: T
authors.lastname: Arpornsuwan
authors.forename: Sunanta
authors.initials: S
authors.lastname: Ratanapo
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002522:Chlorocebus aethiops; D004591:Electrophoresis, Polyacrylamide Gel; D031286:Jatropha / Q000737:chemistry* / Q000196:embryology; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D054789:Ribosome Inactivating Proteins, Type 1 / Q000737:chemistry / Q000378:metabolism*; D012639:Seeds / Q000737:chemistry*; D014709:Vero Cells
chemical_list: D054789:Ribosome Inactivating Proteins, Type 1
keywords: Jatropha curcas; Jc-SCRIP; bioactivities