title: Serum sphingolipids level as a novel potential marker for early detection of human myocardial ischaemic injury.
abstract: BACKGROUND
  Ventricular tachyarrhythmias are the most common and often the first manifestation of coronary heart disease and lead to sudden cardiac death (SCD). Early detection/identification of acute myocardial ischaemic injury at risk for malignant ventricular arrhythmias in patients remains an unmet medical need. In the present study, we examined the sphingolipids level after transient cardiac ischaemia following temporary coronary artery occlusion during percutaneous coronary intervention (PCI) in patients and determined the role of sphingolipids level as a novel marker for early detection of human myocardial ischaemic injury.
  
  
  RESULTS
  Venous samples were collected from either the coronary sinus (n = 7) or femoral vein (n = 24) from 31 patients aged 40-73 years-old at 1, 5 min, and 12 h, following elective PCI. Plasma sphingolipids levels were assessed by HPLC. At 1 min coronary sinus levels of sphingosine 1-phosphate (S1P), sphingosine (SPH), and sphinganine (SA) were increased by 314, 115, and 614%, respectively (n = 7), while peripheral blood levels increased by 79, 68, and 272% (n = 24). By 5 min, coronary sinus S1P and SPH levels increased further (720%, 117%), as did peripheral levels of S1P alone (792%). Where troponin T was detectable at 12 h (10 of 31), a strong correlation was found with peak S1P (R (2) = 0.818; P < 0.0001).
  
  
  CONCLUSIONS
  For the first time, we demonstrate the behavior of plasma sphingolipids following transient cardiac ischaemia in humans. The observation supports the important role of sphingolipids level as a potential novel marker of transient or prolonged myocardial ischaemia.
authors.affiliation: Department of Physiology and Biophysics, Faculty of Medicine, Dalhousie University Halifax, NS, Canada ; Faculty of Medicine and Human Sciences, Institute of Cardiovascular Sciences, University of Manchester Manchester, UK.
authors.forename: Emmanuel E
authors.initials: EE
authors.lastname: Egom
authors.forename: Mamas A
authors.initials: MA
authors.lastname: Mamas
authors.forename: Sanoj
authors.initials: S
authors.lastname: Chacko
authors.forename: Sally E
authors.initials: SE
authors.lastname: Stringer
authors.forename: Valentine
authors.initials: V
authors.lastname: Charlton-Menys
authors.forename: Magdi
authors.initials: M
authors.lastname: El-Omar
authors.forename: Debora
authors.initials: D
authors.lastname: Chirico
authors.forename: Bernard
authors.initials: B
authors.lastname: Clarke
authors.forename: Ludwig
authors.initials: L
authors.lastname: Neyses
authors.forename: J Kennedy
authors.initials: JK
authors.lastname: Cruickshank
authors.forename: Ming
authors.initials: M
authors.lastname: Lei
authors.forename: Farzin
authors.initials: F
authors.lastname: Fath-Ordoubadi
keywords: ischaemia; sphingolipids; sphingosine 1-phosphate