title: Flavonoids from Astragalus membranaceus and their inhibitory effects on LPS-stimulated pro-inflammatory cytokine production in bone marrow-derived dendritic cells.
abstract: Radix Astragali (Astragalus membranaceus) is an important traditional Chinese medicine that is widely used as a tonic to enhance the body's natural defense mechanisms. In this phytochemical study, 12 flavonoids, isoliquiritigenin (1), liquiritigenin (2), calycosin (3), calycosin 7-O-β-D-glucoside (4), formononetin (5), formononetin 7-O-β-D-glucoside (6), daidzein (7), daidzein 7-O-β-D-glucoside (8), methylnissolin (9), methylnissolin 3-O-β-D-glucoside (10), isomucronulatol (11), and isomucronulatol 7-O-β-D-glucoside (12), were isolated from the roots of A. membranaceus. Their structures were elucidated by comparing spectroscopic data with reported values. The effects of the isolated compounds on lipopolysaccharide (LPS)-stimulated bone marrow-derived dendritic cells were investigated. Compounds 1 and 2 exhibited significant inhibitory effects on LPS-induced IL-6 and IL-12 p40 production, with IC50 values ranging from 2.7 to 6.1 μM. Compound 1 also showed a moderate inhibitory effect on LPS-stimulated production of TNF-α with an IC50 value of 20.1 μM. Further studies of the potential anti-inflammatory effects and benefits of flavonoids from A. membranaceus are warranted.
authors.affiliation: College of Pharmacy, Chungnam National University, Daejeon, 305-764, Korea.
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.forename: Ya Nan
authors.initials: YN
authors.lastname: Sun
authors.forename: Xi Tao
authors.initials: XT
authors.lastname: Yan
authors.forename: Seo Young
authors.initials: SY
authors.lastname: Yang
authors.forename: Sohyun
authors.initials: S
authors.lastname: Kim
authors.forename: Young Mi
authors.initials: YM
authors.lastname: Lee
authors.forename: Young-Sang
authors.initials: YS
authors.lastname: Koh
authors.forename: Young Ho
authors.initials: YH
authors.lastname: Kim
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000302:isolation & purification / Q000494:pharmacology*; D027883:Astragalus Plant / Q000737:chemistry*; D001854:Bone Marrow Cells / Q000166:cytology; D018929:Cell Culture Techniques; D016207:Cytokines / Q000037:antagonists & inhibitors*; D003713:Dendritic Cells / Q000187:drug effects* / Q000276:immunology; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology*; D005419:Flavonoids / Q000302:isolation & purification / Q000494:pharmacology*; D008070:Lipopolysaccharides / Q000494:pharmacology*; D051379:Mice; D008810:Mice, Inbred C57BL; D018517:Plant Roots / Q000737:chemistry
chemical_list: D000893:Anti-Inflammatory Agents; D016207:Cytokines; D004365:Drugs, Chinese Herbal; D005419:Flavonoids; D008070:Lipopolysaccharides