title: Resveratrol exhibits differential protective effects on fast- and slow-twitch muscles in streptozotocin-induced diabetic rats.
abstract: BACKGROUND
  This study aimed to investigate the differential protective effect of resveratrol (RSV) on oxidative stress and metabolic signaling pathways in fast- and slow-twitch skeletal muscles of rats with diabetes.
  
  
  METHODS
  Diabetic rats were induced by streptozotocin (STZ) for 2 weeks and then administered with RSV (1, 10 and 100 μg/kg per day) for 1 week. We determined oxidative stress and protein expression by lucigenin-mediated chemiluminescence and Western immunoblot.
  
  
  RESULTS
  The superoxide anion production and copper-zinc superoxide dismutase (CuZnSOD) protein level were increased in fast-twitch muscle than in slow-twitch muscle of diabetes. The Akt and glycogen synthase kinase 3 (GSK-3) phosphorylations were reduced in both fast- and slow-twitch muscles of diabetes. Oxidative stress and GSK-3 dephosphorylation were corrected by RSV treatment in both fast- and slow-twitch muscles of diabetes. Furthermore, RSV treatment downregulated CuZnSOD protein level in diabetic fast-twitch muscle. In diabetic slow-twitch muscle, RSV treatment elevated manganese SOD (MnSOD) and phosphorylated Akt protein levels and reduced acetyl-CoA carboxylase (ACC) phosphorylation.
  
  
  CONCLUSIONS
  Our results suggested that fast-twitch muscle incurred more oxidative stress, whereas slow-twitch muscle altered metabolic signaling molecules activities under diabetic status. The antidiabetic effect of RSV on fast- and slow-twitch skeletal muscles was mediated by different antioxidative and metabolic signals.
authors.affiliation: Department of Clinical Pathology, Far Eastern Memorial Hospital, Taipei.
authors.forename: Chih-Chun
authors.initials: CC
authors.lastname: Chang
authors.forename: Meng-Hsuan
authors.initials: MH
authors.lastname: Yang
authors.forename: Hung-Chun
authors.initials: HC
authors.lastname: Tung
authors.forename: Chieh-Yu
authors.initials: CY
authors.lastname: Chang
authors.forename: Yu-Lin
authors.initials: YL
authors.lastname: Tsai
authors.forename: Jiung-Pang
authors.initials: JP
authors.lastname: Huang
authors.forename: Tzung-Hai
authors.initials: TH
authors.lastname: Yen
authors.forename: Li-Man
authors.initials: LM
authors.lastname: Hung
mesh_terms: D000103:Acetyl-CoA Carboxylase / Q000378:metabolism; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001786:Blood Glucose / Q000378:metabolism; D015153:Blotting, Western; D001835:Body Weight; D002784:Cholesterol / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism; D038362:Glycogen Synthase Kinase 3 / Q000378:metabolism; D007328:Insulin / Q000378:metabolism; D008297:Male; D018656:Muscle Fibers, Fast-Twitch / Q000187:drug effects*; D018657:Muscle Fibers, Slow-Twitch / Q000187:drug effects*; D018384:Oxidative Stress / Q000187:drug effects; D010766:Phosphorylation / Q000187:drug effects; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D051381:Rats; D020318:Rats, Long-Evans; D000077185:Resveratrol; D015398:Signal Transduction / Q000187:drug effects; D013267:Stilbenes / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000378:metabolism; D013481:Superoxides / Q000378:metabolism; D014280:Triglycerides / Q000378:metabolism
chemical_list: D000975:Antioxidants; D001786:Blood Glucose; D007328:Insulin; D013267:Stilbenes; D014280:Triglycerides; D013481:Superoxides; D002784:Cholesterol; D013482:Superoxide Dismutase; D051057:Proto-Oncogene Proteins c-akt; D038362:Glycogen Synthase Kinase 3; D000103:Acetyl-CoA Carboxylase; D000077185:Resveratrol
keywords: diabetic myopathy; glycogen synthase kinase 3; oxidative stress; resveratrol; type 1 diabetes; 糖尿病肌病，糖原合成酶激酶3，氧化应激，白藜芦醇，1型糖尿病