title: Effects of UV-B irradiation on the levels of anthocyanin, rutin and radical scavenging activity of buckwheat sprouts.
abstract: The effects of various light compositions on the levels of anthocyanin, rutin and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity in buckwheat (Fagopyrum esculentum Moench) sprouts were evaluated. Dark-grown 6-day-old buckwheat sprouts were irradiated with different sources of visible and ultraviolet (UV) light. Particularly, we examined the effect of UV-B at wavelengths of 260-320 nm, 280-320 nm, and 300-320 nm on the production of flavonoid compounds, using multiple fluorescent lights and cylinders that filter out certain portions of the UV-B. The results showed that irradiation with UV-B>300 nm increased the levels of anthocyanin and rutin, as well as the DPPH radical scavenging activity. When sprouts were irradiated with UV-B light at wavelengths of 260-300 nm, yellowing or withering occurred within 24h of irradiation, indicating that wavelengths in this range are detrimental to the growth of buckwheat sprouts.
authors.affiliation: Shimane University, 1060 Nishikawatsu-cho, Matsue, Shimane 690-8504, Japan. ytsurunaga@edu.shimane-u.ac.jp
authors.forename: Yoko
authors.initials: Y
authors.lastname: Tsurunaga
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Takahashi
authors.forename: Takuya
authors.initials: T
authors.lastname: Katsube
authors.forename: Akihide
authors.initials: A
authors.lastname: Kudo
authors.forename: Osamu
authors.initials: O
authors.lastname: Kuramitsu
authors.forename: Masaki
authors.initials: M
authors.lastname: Ishiwata
authors.forename: Shingo
authors.initials: S
authors.lastname: Matsumoto
mesh_terms: D000872:Anthocyanins / Q000032:analysis*; D019612:Fagopyrum / Q000737:chemistry* / Q000254:growth & development / Q000528:radiation effects*; D016166:Free Radical Scavengers / Q000032:analysis*; D018525:Germination / Q000528:radiation effects; D010936:Plant Extracts / Q000032:analysis*; D012431:Rutin / Q000032:analysis*; D012639:Seeds / Q000737:chemistry / Q000254:growth & development* / Q000528:radiation effects; D014466:Ultraviolet Rays
chemical_list: D000872:Anthocyanins; D016166:Free Radical Scavengers; D010936:Plant Extracts; D012431:Rutin