title: Surface engineering for long-term culturing of mesenchymal stem cell microarrays.
abstract: The cell microarray format can recreate a multitude of cell microenvironments on a single chip using only minimal amounts of reagent. In this study, we describe surface modifications to passivate cell microarrays, aiming to adapt the platform to the study of stem cell behavior over long-term culture periods. Functionalization of glass slides with (3-glycidyloxypropyl) trimethoxysilane enabled covalent anchoring of extracellular matrix proteins on microscale spots printed by a robotic contact printer. Subsequently, the surface was passivated by bovine serum albumin (BSA) or poly(ethylene glycol)bisamine (A-PEG) with molecular weights of 3000, 6000, and 10 000 Da. Cloud-point conditions for A-PEG grafting were attained that were compatible with protein deposition. Passivation strategies were assessed by culturing mesenchymal stem cells on the microarray platform. While both BSA and A-PEG passivation initially blocked cell adhesion between the printed spots, only A-PEG grafting was able to maintain cell pattern integrity over the entire culture period of 3 weeks.
authors.affiliation: Mawson Institute, University of South Australia, Australia.
authors.forename: Soraya
authors.initials: S
authors.lastname: Rasi Ghaemi
authors.forename: Frances
authors.initials: F
authors.lastname: Harding
authors.forename: Bahman
authors.initials: B
authors.lastname: Delalat
authors.forename: Roshan
authors.initials: R
authors.lastname: Vasani
authors.forename: Nicolas H
authors.initials: NH
authors.lastname: Voelcker
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D002470:Cell Survival; D016326:Extracellular Matrix Proteins / Q000737:chemistry*; D006801:Humans; D055767:Immobilized Proteins / Q000737:chemistry*; D019169:Jurkat Cells; D059630:Mesenchymal Stem Cells / Q000502:physiology*; D011092:Polyethylene Glycols / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D013431:Sulfates / Q000737:chemistry; D013499:Surface Properties; D046888:Tissue Array Analysis / Q000379:methods
chemical_list: D016326:Extracellular Matrix Proteins; D055767:Immobilized Proteins; D013431:Sulfates; C031512:potassium sulfate; D011092:Polyethylene Glycols