title: A simple and precise method for quantitative analysis of lumefantrine by planar chromatography.
abstract: A simple, precise and sensitive high performance thin layer chromatographic (HPTLC) method has been developed and validated for drug of choice Lumefantrine in treatment of malaria (P. falciparum). Silica gel 60 F254 HPTLC precoated plates were used for quantitative analytical purpose. Methanol water 9.5 + 0.5 (v/v) was used as the solvent system. Densitometric scanning was carried out with deuterium lamp set at detection wavelength of 266 nm. The response to lumefantrine concentration was linear in the concentration range of 1.25-12.50 μg/ml. The suitability of the method developed and validated was in accordance with the requirements of the ICH guidelines (Q2B). Thus the validated method can be further applied to quantitative analysis of lumefantrine in commercial pharmaceutical dosage form. The proposed method is simple, sensitive, precise and accurate, confirming its pharmaceutical application in routine quality control analysis.
authors.affiliation: Department of Pharmaceutical Analysis, Prin. K. M. Kundnani College of Pharmacy, Jote Joy Building, Rambhau Salgaonkar Marg, Cuffe Parade, Colaba, Mumbai, India.
authors.forename: Purnima
authors.initials: P
authors.lastname: Hamrapurkar
authors.forename: Mitesh
authors.initials: M
authors.lastname: Phale
authors.forename: Sandeep
authors.initials: S
authors.lastname: Pawar
authors.forename: Priti
authors.initials: P
authors.lastname: Patil
authors.forename: Mittal
authors.initials: M
authors.lastname: Gandhi
keywords: Densitometric estimation; HPTLC; lumefantrine