title: Pharmacodynamic evaluation for antiplasmodial activity of Holarrhena antidysentrica (Kutaja) and Azadirachta indica (Neemb) in Plasmodium berghei infected mice model.
abstract: OBJECTIVE
  To investigate in-vivo anti-plasmodial activity of aqueous extracts of plants selected based on the symptomology mentioned in Ayurveda.
  
  
  METHODS
  The aqueous extracts of Holarrhena antidysentrica (H. antidysentrica) (Kutaja) and Azadirachta indica (A. indica) (Neemb) for their antiplasmodial potential in Plasmodium berghei (P. berghei) infected mice was assessed using Peters four day suppressive test. Both the extracts were administered at 2 dose levels, full dose (1 000 mg/d) and minimized dose (200 mg/d). 10(6)P. berghei infected RBCs were injected on day '0' and treated from day '0' till day '3' post-infection. Tail blood smears were collected, giemsa stained and analyzed. The mice were observed for survival and parasitemia was assessed till 50% of mice in control survived.
  
  
  RESULTS
  It was observed that the percentage of parasitemia increased gradually in all the groups, with maximum in control group (Day 3-35, Day 9-46.98) and minimum in Chloroquine arm (Day 3-14.06, Day 9-19.92). The percentage of parasitemia was compared using Mann-Whitney U test depicting that all test groups exhibited reduction in parasitemia as compared to control (P-value<0.002 for all groups). These groups showed similar percentage of survival as Chloroquine.
  
  
  CONCLUSIONS
  The present investigation demonstrated the anti-plasmodial effects of H. antidysentrica and A. indica, which are two most commonly used medicinal plants in Ayurved for treatment of fever.
authors.affiliation: University Department of Infectious Diseases and University Department of Interpathy Research and Technology, Maharashtra University of Health Sciences, Mumbai, India. priyanka.ing@gmail.com
authors.forename: Jadhav
authors.initials: J
authors.lastname: Priyanka
authors.forename: Lal
authors.initials: L
authors.lastname: Hingorani
authors.forename: Kshirsagar
authors.initials: K
authors.lastname: Nilima
mesh_terms: D000818:Animals; D000962:Antimalarials / Q000494:pharmacology*; D031601:Azadirachta; D035462:Holarrhena; D008288:Malaria / Q000188:drug therapy*; D008297:Male; D008515:Medicine, Ayurvedic; D051379:Mice; D018512:Parasitemia / Q000188:drug therapy; D008517:Phytotherapy / Q000379:methods*; D024301:Plant Bark; D010936:Plant Extracts / Q000494:pharmacology; D018515:Plant Leaves; D018547:Plant Stems; D010946:Plants, Medicinal; D010962:Plasmodium berghei; D011897:Random Allocation
chemical_list: D000962:Antimalarials; D010936:Plant Extracts