title: Nucleosomal TATA-switch: competing orientations of TATA on the nucleosome.
abstract: Transcription is known to be affected by the rotational setting of the transcription response elements within nucleosomes. We studied the rotational positioning of the TATA box, the most universal promoter motif. We applied a bioinformatic high-resolution nucleosome mapping technique to eukaryotic promoters. Our results show that the nucleosome DNA sequence harboring the TATA box encodes alternative rotational positions for the same piece of DNA. This may serve for switching the gene activity on and off.
authors.affiliation: Genome Diversity Center, Institute of Evolution, University of Haifa, Mount Carmel, 31905 Haifa, Israel. hapala@mail.muni.cz
authors.forename: Jan
authors.initials: J
authors.lastname: Hapala
authors.forename: Edward N
authors.initials: EN
authors.lastname: Trifonov
mesh_terms: D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D005786:Gene Expression Regulation; D006801:Humans; D008957:Models, Genetic; D009707:Nucleosomes / Q000235:genetics*; D010944:Plants / Q000235:genetics; D017422:Sequence Analysis, DNA; D016385:TATA Box
chemical_list: D009707:Nucleosomes
keywords: A; Chromatin and transcription; Eukaryotic promoter; N; Nucleosome mapping; Rotational positioning; T; TATA; TATA box; TATA box binding protein; TATA box: regulatory sequence within promoters with consensual sequence TATAAA; TBP; TSS; adenine; any nucleotide (A, C, G or T); hSWI/SNF; human SWI/SNF: (SWItch/Sucrose NonFermentable) chromatin remodeling protein complex; nt; nucleotide(s); thymine; transcription start site