title: Formation of membrane ridges and scallops by the F-BAR protein Nervous Wreck.
abstract: Eukaryotic cells are defined by extensive intracellular compartmentalization, which requires dynamic membrane remodeling. FER/Cip4 homology-Bin/amphiphysin/Rvs (F-BAR) domain family proteins form crescent-shaped dimers, which can bend membranes into buds and tubules of defined geometry and lipid composition. However, these proteins exhibit an unexplained wide diversity of membrane-deforming activities in vitro and functions in vivo. We find that the F-BAR domain of the neuronal protein Nervous Wreck (Nwk) has a novel higher-order structure and membrane-deforming activity that distinguishes it from previously described F-BAR proteins. The Nwk F-BAR domain assembles into zigzags, creating ridges and periodic scallops on membranes in vitro. This activity depends on structural determinants at the tips of the F-BAR dimer and on electrostatic interactions of the membrane with the F-BAR concave surface. In cells, Nwk-induced scallops can be extended by cytoskeletal forces to produce protrusions at the plasma membrane. Our results define a new F-BAR membrane-deforming activity and illustrate a molecular mechanism by which positively curved F-BAR domains can produce a variety of membrane curvatures. These findings expand the repertoire of F-BAR domain mediated membrane deformation and suggest that unique modes of higher-order assembly can define how these proteins sculpt the membrane.
authors.affiliation: Rosenstiel Basic Medical Sciences Research Center, Department of Biology, Brandeis University, Waltham, MA 02454, USA.
authors.forename: Agata N
authors.initials: AN
authors.lastname: Becalska
authors.forename: Charlotte F
authors.initials: CF
authors.lastname: Kelley
authors.forename: Cristina
authors.initials: C
authors.lastname: Berciu
authors.forename: Tatiana B
authors.initials: TB
authors.lastname: Stanishneva-Konovalova
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Fu
authors.forename: Shiyu
authors.initials: S
authors.lastname: Wang
authors.forename: Olga S
authors.initials: OS
authors.lastname: Sokolova
authors.forename: Daniela
authors.initials: D
authors.lastname: Nicastro
authors.forename: Avital A
authors.initials: AA
authors.lastname: Rodal
mesh_terms: D000818:Animals; D002460:Cell Line; D021961:Cell Membrane Structures / Q000378:metabolism* / Q000648:ultrastructure; D003198:Computer Simulation; D029721:Drosophila Proteins / Q000737:chemistry / Q000502:physiology*; D004331:Drosophila melanogaster; D006801:Humans; D008081:Liposomes / Q000737:chemistry; D008958:Models, Molecular; D009419:Nerve Tissue Proteins / Q000737:chemistry / Q000502:physiology*; D011485:Protein Binding; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary
chemical_list: D029721:Drosophila Proteins; D008081:Liposomes; D009419:Nerve Tissue Proteins; C483010:nwk protein, Drosophila