title: A phytotoxin Solanapyrone-A downregulates calcium-dependent protein kinase activity in potato.
abstract: We previously demonstrated that alternaric acid, a host-specific toxin produced by the plant pathogenic fungus Alternaria solani, in the presence of Ca(2+) and Mg(2+), stimulated in vitro phosphorylation of His-tagged calcium-dependent protein kinase 2 from potato cultivar Rishiri (RiCDPK2). Herein, we report that Solanapyrone-A (SpA), a non-host-specific toxin produced by A. solani, inhibited the phosphorylation of RiCDPK2 in the presence of Ca(2+) and Mg(2+). However, SpA stimulated RiCDPK2 phosphorylation in the absence of these cations. Based on the current findings, we suggest that RiCDPK2 may mediate SpA-induced signaling independent of Ca(2+) and Mg(2+), leading to a compatible interaction between potato and A. solani.
authors.affiliation: Biotechnology Program, Department of Environmental Sciences, COMSATS Institute of Information Technology, Abbottabad, Pakistan.
authors.forename: A
authors.initials: A
authors.lastname: Hassan
authors.forename: N
authors.initials: N
authors.lastname: Hatsugai
authors.forename: M M
authors.initials: MM
authors.lastname: Shah
mesh_terms: D002118:Calcium / Q000494:pharmacology; D015536:Down-Regulation / Q000187:drug effects*; D018551:Solanum lycopersicum / Q000187:drug effects; D008274:Magnesium / Q000494:pharmacology; D009281:Naphthalenes / Q000494:pharmacology*; D010766:Phosphorylation; D018515:Plant Leaves / Q000187:drug effects; D011494:Protein Kinases / Q000378:metabolism*; D011753:Pyrones / Q000494:pharmacology*; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D011198:Solanum tuberosum / Q000187:drug effects* / Q000201:enzymology*; D017871:Calcium-Calmodulin-Dependent Protein Kinases
chemical_list: D002118:Calcium; D008274:Magnesium; D009281:Naphthalenes; D011494:Protein Kinases; D011753:Pyrones; D011993:Recombinant Fusion Proteins; D017871:Calcium-Calmodulin-Dependent Protein Kinases; C113266:solanapyrone A