title: Up-regulated expression of Bnip3L after intracerebral hemorrhage in adult rats.
abstract: Bnip3L, also known as NIX, is a homolog of the E1B 19K/Bcl-2 binding and pro-apoptotic protein Bnip3 which can bind to Bcl-2 to elaborate that effect. In tumor cells, Bnip3L played a role in tumor growth inhibition, but some studies argued hypoxia-induced autophagy via Bnip3L was a survival mechanism that promoted tumor progression. In heart muscle, it related to decreased myocardial function. However, its function in intracerebral hemorrhage (ICH) is still not clear. In this frame, we found the Bnip3L expression increased in the perihematomal region in adult rats after performed ICH. Double immunofluorenscence staining manifested that Bnip3L co-located with neurons, not astrocytes or oligodendrocytes. Furthermore, we detected that neuronal apoptosis marker active caspase-3 had colocalizations with Bnip3L. In addition, colocalizations and co-immunoprecipitation between Bnip3L and Bcl-2, consistent with previous study, were also found. All our findings suggested that Bnip3L might be involved in the pathophysiology of ICH.
authors.affiliation: Department of Neurology, Affiliated Hospital of Nantong University, Nantong, 226001, Jiangsu Province, People's Republic of China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Rui
authors.forename: Kaifu
authors.initials: K
authors.lastname: Ke
authors.forename: Lei
authors.initials: L
authors.lastname: Li
authors.forename: Heyi
authors.initials: H
authors.lastname: Zheng
authors.forename: Wei
authors.initials: W
authors.lastname: Xu
authors.forename: Xiang
authors.initials: X
authors.lastname: Tan
authors.forename: Jianhua
authors.initials: J
authors.lastname: Cao
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Wu
authors.forename: Gang
authors.initials: G
authors.lastname: Cui
authors.forename: Guangwei
authors.initials: G
authors.lastname: Zhao
authors.forename: Yilu
authors.initials: Y
authors.lastname: Gao
authors.forename: Maohong
authors.initials: M
authors.lastname: Cao
mesh_terms: D000818:Animals; D017209:Apoptosis; D001522:Behavior, Animal; D001921:Brain / Q000098:blood supply / Q000378:metabolism* / Q000473:pathology; D053148:Caspase 3 / Q000235:genetics / Q000378:metabolism; D002543:Cerebral Hemorrhage / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D005786:Gene Expression Regulation; D047468:Immunoprecipitation; D008297:Male; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D009474:Neurons / Q000378:metabolism* / Q000473:pathology; D009483:Neuropsychological Tests; D011518:Proto-Oncogene Proteins / Q000235:genetics* / Q000378:metabolism; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: C505659:BNIP3L protein, rat; D008565:Membrane Proteins; D011518:Proto-Oncogene Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D053148:Caspase 3