title: Spectrophotometric methods for simultaneous estimation of rabeprazole sodium and aceclofenac from the combined capsule dosage form.
abstract: BACKGROUND
  The present work aimed to develop and validate spectrophotometric methods for simultaneous estimation of rabeprazole sodium and aceclofenac in a pure and capsule dosage form.
  
  
  METHODS
  Method 1 is based on solving a simultaneous equation. Absorbances of rabeprazole sodium and aceclofenac were measured at their respective absorbance maximas (λ max) of 283 and 276 nm. Method 2 is the Q-analysis or absorption ratio method. Absorbances were measured at 256 nm (isosbestic point) and 276 nm (λ max of aceclofenac). Methods are validated according to ICH guidelines.
  
  
  RESULTS
  A linearity range for rabeprazole sodium and aceclofenac is 10-60 μg/ml at respective selected wavelengths. The coefficient of correlation for rabeprazole at 283 nm and for aceclofenac at 276 nm is 0.9981 and 0.9997, respectively. A percentage estimation of rabeprazole sodium and aceclofenac from the capsule dosage form by method 1 is 100.22 and 99.96 and by method 2 is 99.99 and 100.05, respectively, with a standard deviation less than 2.
  
  
  CONCLUSIONS
  The proposed methods are simple, rapid, and validated and can be used successfully for routine simultaneous estimation of rabeprazole sodium and aceclofenac in a pure and capsule dosage form.
authors.affiliation: Department of Pharmaceutical Chemistry and PG Studies, P.D.V.V.P.F's College of Pharmacy, Vilad Ghat, Ahmednagar, India.
authors.forename: Ramesh L
authors.initials: RL
authors.lastname: Sawant
authors.forename: Sanket D
authors.initials: SD
authors.lastname: Hadawale
authors.forename: Ganesh K
authors.initials: GK
authors.lastname: Dhikale
authors.forename: Charusheela A
authors.initials: CA
authors.lastname: Bansode
authors.forename: Pravin S
authors.initials: PS
authors.lastname: Tajane
keywords: Aceclofenac; absorbance ratio method; rabeprazole sodium; simultaneous equation method; spectrometric estimation