title: Cultured allogenic keratinocytes for extensive burns: a retrospective study over 15 years.
abstract: The aim was to review the use and indications of cultured allogenic keratinocytes (CAlloK) in extensive burns and their efficiency.
  
  
  METHODS
  This retrospective study comprised 15 years (1997-2012).
  
  
  METHODS
  all patients who received CAlloK.
  
  
  METHODS
  patients who died before complete healing. Evaluation criteria were clinical. Time and success of wound healing after CAlloK use were evaluated.
  
  
  RESULTS
  The CAlloK were used for 2 indications - STSG donor sites and deep 2nd degree burns in extensively burned patients. A total of 70 patients were included with severity Baux score of 99.2 (from 51 to 144) and mean percentage of TBSA of 63.49% (from 21 to 96%). Fifty nine patients received CAlloK for STSG donor sites with a mean number of applications of 4 and mean surface of 3800 cm(2) per patient. Treated donor sites were re-harvested 2.5 times. The mean time of complete epithelialization was 7 days. In 11 patients, CAlloK were used for deep 2nd degree burns. The mean percentage of burned surface was 73.7%. The mean surface of CAlloK per patient was 2545 cm(2). Complete healing was achieved in 6.4 days.
  
  
  CONCLUSIONS
  The CAlloK allow rapid healing of STSG donor-sites and deep 2nd second degree burns in extensively burned patients.
authors.affiliation: Bank of Skin Substitutes, Edouard Herriot Hospital, Lyon, France; Department of Burns and Plastic and Reconstructive Surgery, Edouard Herriot Hospital, Lyon, France.
authors.forename: Celine
authors.initials: C
authors.lastname: Auxenfans
authors.affiliation: Bank of Skin Substitutes, Edouard Herriot Hospital, Lyon, France; Department of Burns and Plastic and Reconstructive Surgery, Edouard Herriot Hospital, Lyon, France. Electronic address: cshipkov@gmail.com.
authors.forename: Hristo
authors.initials: H
authors.lastname: Shipkov
authors.affiliation: Bank of Skin Substitutes, Edouard Herriot Hospital, Lyon, France; Department of Burns and Plastic and Reconstructive Surgery, Edouard Herriot Hospital, Lyon, France.
authors.forename: Christine
authors.initials: C
authors.lastname: Bach
authors.affiliation: Bank of Skin Substitutes, Edouard Herriot Hospital, Lyon, France; Department of Burns and Plastic and Reconstructive Surgery, Edouard Herriot Hospital, Lyon, France.
authors.forename: Zulma
authors.initials: Z
authors.lastname: Catherine
authors.affiliation: Bank of Skin Substitutes, Edouard Herriot Hospital, Lyon, France; Department of Burns and Plastic and Reconstructive Surgery, Edouard Herriot Hospital, Lyon, France.
authors.forename: Pierre
authors.initials: P
authors.lastname: Lacroix
authors.affiliation: Bank of Skin Substitutes, Edouard Herriot Hospital, Lyon, France; Department of Burns and Plastic and Reconstructive Surgery, Edouard Herriot Hospital, Lyon, France.
authors.forename: Marc
authors.initials: M
authors.lastname: Bertin-Maghit
authors.affiliation: Bank of Skin Substitutes, Edouard Herriot Hospital, Lyon, France; Department of Burns and Plastic and Reconstructive Surgery, Edouard Herriot Hospital, Lyon, France.
authors.forename: Odile
authors.initials: O
authors.lastname: Damour
authors.affiliation: Bank of Skin Substitutes, Edouard Herriot Hospital, Lyon, France; Department of Burns and Plastic and Reconstructive Surgery, Edouard Herriot Hospital, Lyon, France.
authors.forename: Fabienne
authors.initials: F
authors.lastname: Braye
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002056:Burns / Q000601:surgery* / Q000628:therapy; D018929:Cell Culture Techniques; D017690:Cell Transplantation / Q000379:methods*; D002478:Cells, Cultured / Q000637:transplantation; D002648:Child; D002675:Child, Preschool; D015331:Cohort Studies; D005260:Female; D006801:Humans; D007223:Infant; D015603:Keratinocytes / Q000637:transplantation*; D008297:Male; D008875:Middle Aged; D012189:Retrospective Studies; D012720:Severity of Illness Index; D016038:Skin Transplantation / Q000379:methods*; D060053:Transplant Donor Site; D014184:Transplantation, Homologous; D016896:Treatment Outcome; D014945:Wound Healing; D055815:Young Adult
keywords: Cultured allogenic kearatinocytes; Deep second degree burns; STSG donor sites