title: Nonenzymatic labeling of 5-hydroxymethylcytosine in nanopore sequencing.
abstract: Labeling chemically for a "single" result: A one-step, nonenzymatic 5hmC thiolation method mediated by bisulfite is highlighted. The 5hmC base can be chemically labeled in single-stranded DNA and detected by using nanopore sequencing.
authors.affiliation: Department of Chemistry, University of Chicago, 929 East 57th Street, Chicago, IL 60637, USA.
authors.forename: Xingyu
authors.initials: X
authors.lastname: Lu
authors.forename: Chuan
authors.initials: C
authors.lastname: He
mesh_terms: D044503:5-Methylcytosine / Q000031:analogs & derivatives; D003596:Cytosine / Q000031:analogs & derivatives* / Q000737:chemistry; D019175:DNA Methylation; D004277:DNA, Single-Stranded / Q000737:chemistry* / Q000378:metabolism; D058608:Nanopores; D017422:Sequence Analysis, DNA
chemical_list: D004277:DNA, Single-Stranded; C011865:5-hydroxymethylcytosine; D044503:5-Methylcytosine; D003596:Cytosine
keywords: 5-hydroxylmethylcytosine; DNA methylation; epigenetics; nanopores; nonenzymatic labeling