title: The RP-HPLC method for simultaneous estimation of esomeprazole and naproxen in binary combination.
abstract: OBJECTIVE
  A simple, precise, reliable, rapid, sensitive and validated RP-HPLC method has been developed to determine esomeprazole magnesium trihydrate (ESO) and naproxen (NAP) in synthetic mixture form.
  
  
  METHODS
  Chromatographic separation achieved isocratically on Phenomenex, Luna C18 column (5 μm, 150mm × 4.60mm) and acetonitrile: phosphate buffer (pH 7.0) in the ratio of 50:50 (v/v) as the mobile phase, at a flow rate of 0.5 ml/min. Detection was carried out at 300 nm. The retention times for NAP and ESO was found to be 2.67 ±0.014 and 5.65 ±0.09 min respectively. Parameters such as linearity, precision, accuracy, recovery, specificity and ruggedness are studied as reported in the ICH guidelines.
  
  
  RESULTS
  The method was linear in the concentration range of 50-250 μg/ml for NAP and 2-10 μg/ml for ESO with correlation coefficient of 0.999 and 0.998 respectively. The mean recoveries obtained for NAP and ESO were 100.01% and 97.76 % respectively and RSD was less than 2. The correlation coefficients for all components are close to 1.
  
  
  CONCLUSIONS
  Developed method was found to be accurate, precise, selective and rapid for simultaneous estimation of NAP and ESO.
authors.affiliation: Truba Institute of Pharmacy, Karond Gandhi Nagar Bypass Road, Bhopal, India.
authors.forename: Deepak Kumar
authors.initials: DK
authors.lastname: Jain
authors.forename: Nitesh
authors.initials: N
authors.lastname: Jain
authors.forename: Rita
authors.initials: R
authors.lastname: Charde
authors.forename: Nilesh
authors.initials: N
authors.lastname: Jain
keywords: Esomeprazole magnesium trihydrate; RP-HPLC; naproxen; validation