title: Screening of genes regulated by Relish in Chinese shrimp Fenneropenaeus chinensis.
abstract: Relish is a key NF-κB transcription factor in the innate immunity. Learning the function of Relish in regulating the related genes of shrimp will be helpful to understand the shrimp immunity. In the present study, RNA interference (RNAi) and suppression subtractive hybridization (SSH) techniques were combined together to identify the genes regulated by Relish. A forward SSH library represents the genes whose transcription was regulated by Relish, and the reverse SSH library represents the genes whose transcription was up-regulated after Relish was silenced in shrimp responsive to Vibrio anguillarium (VA) stimulation. In the forward library, 43 unique genes were identified, and in the reverse library, 57 genes were identified. The expression of ten differentially expressed genes, including early cuticle protein5 (ECP5), Toll-like receptor protein (TLRP), antiviral factor (AV), C-type lectin receptor (CLR), thrombospondin (TSP), S-adenosylmethionine synthetase (SAMS), carcinolectin 5b-5 (CL5b-5), QM protein (QMP), heat shock protein 67B2 (HSP67B2), and Thioredoxin-related protein 14 (TRP14) were further confirmed by real-time PCR. The present data provides us a wide view to understand the function of Relish gene in the innate immunity of shrimp.
authors.affiliation: Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China.
authors.forename: Dongdong
authors.initials: D
authors.lastname: Wang
authors.forename: Shihao
authors.initials: S
authors.lastname: Li
authors.forename: Fuhua
authors.initials: F
authors.lastname: Li
mesh_terms: D000818:Animals; D060829:Arthropod Proteins / Q000235:genetics*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D015723:Gene Library; D054884:Host-Pathogen Interactions; D016328:NF-kappa B / Q000235:genetics; D009693:Nucleic Acid Hybridization / Q000379:methods; D033561:Penaeidae / Q000235:genetics* / Q000382:microbiology; D034622:RNA Interference; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014157:Transcription Factors / Q000235:genetics*; D014733:Vibrio / Q000502:physiology
chemical_list: D060829:Arthropod Proteins; D016328:NF-kappa B; D014157:Transcription Factors
keywords: RNAi; Relish; SSH; Shrimp immunity