title: Cembrane derivatives from the soft corals, Sinularia gaweli and Sinularia flexibilis.
abstract: A new norcembranoidal diterpene, 1-epi-sinulanorcembranolide A (1), and a new cembranoidal diterpene, flexibilin D (2), were isolated from the soft corals, Sinularia gaweli and Sinularia flexibilis, respectively. The structures of new metabolites 1 and 2 were elucidated by spectroscopic methods, and compound 2 was found to significantly inhibit the accumulation of the pro-inflammatory iNOS and COX-2 proteins of the lipopolysaccharide (LPS)-stimulated RAW264.7 macrophage cells. In addition, S. flexibilis yielded a known cembrane, 5-dehydrosinulariolide (3); the structure, including its absolute stereochemistry, was further confirmed by single-crystal X-ray diffraction analysis.
authors.affiliation: Graduate Institute of Marine Biotechnology and Department of Life Science and Institute of Biotechnology, National Dong Hwa University, Pingtung 944, Taiwan.
authors.forename: Li-Chung
authors.initials: LC
authors.lastname: Hu
authors.forename: Wei-Hsuan
authors.initials: WH
authors.lastname: Yen
authors.forename: Jui-Hsin
authors.initials: JH
authors.lastname: Su
authors.forename: Michael Yen-Nan
authors.initials: MY
authors.lastname: Chiang
authors.forename: Zhi-Hong
authors.initials: ZH
authors.lastname: Wen
authors.forename: Wu-Fu
authors.initials: WF
authors.lastname: Chen
authors.forename: Ting-Jang
authors.initials: TJ
authors.lastname: Lu
authors.forename: Yu-Wei
authors.initials: YW
authors.lastname: Chang
authors.forename: Yung-Husan
authors.initials: YH
authors.lastname: Chen
authors.forename: Wei-Hsien
authors.initials: WH
authors.lastname: Wang
authors.forename: Yang-Chang
authors.initials: YC
authors.lastname: Wu
authors.forename: Ping-Jyun
authors.initials: PJ
authors.lastname: Sung
mesh_terms: D000818:Animals; D037421:Anthozoa / Q000737:chemistry*; D051546:Cyclooxygenase 2 / Q000187:drug effects / Q000378:metabolism; D004224:Diterpenes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D052247:Nitric Oxide Synthase Type II / Q000187:drug effects / Q000378:metabolism; D013057:Spectrum Analysis; D014961:X-Ray Diffraction
chemical_list: D004224:Diterpenes; D008070:Lipopolysaccharides; D052247:Nitric Oxide Synthase Type II; D051546:Cyclooxygenase 2