title: The senescence-accelerated mouse-prone 8 is not a suitable model for the investigation of cardiac inflammation and oxidative stress and their modulation by dietary phytochemicals.
abstract: Aging is associated with chronic inflammation and oxidative stress, which both may promote age-associated disorders including cardiovascular diseases. The cardiovascular system suffers from the life-long impact of stressors, such as reactive oxygen and nitrogen species. A diet rich in vegetables and fruits, and thus phytochemicals, may extend healthy lifespan in humans, in part by improving heart health by lowering of oxidative stress and modulating signal transduction pathways. To investigate the potential impact of dietary anthocyanin-rich bilberry extract and curcumin on oxidative stress and inflammatory markers in the heart, two groups of senescence-accelerated mouse-resistant 1 (SAMR1) and senescence-accelerated mouse-prone 8 (SAMP8) mice, respectively, were fed a Western-type diet (normal control and aged control, respectively) and two groups of SAMP8 mice were fed either bilberry extract (20g/kg diet) or curcumin (500mg/kg diet) over a period of 5 months. An activation of the transcription factor nuclear factor κ B (NFκB), but no differences in the gene and protein expression of NFκB-regulated pro-inflammatory mediators, was observed in the hearts of SAMP8 compared to SAMR1 control mice. Cardiac concentrations of protein and lipid oxidation parameters were similar in SAMR1 and SAMP8 control mice and the phytochemical-fed SAMP8 mice. Our data question the suitability of the SAMP8 and SAMR1 strains as a model for age-dependent changes of pro-inflammatory cytokines and oxidative stress in the heart.
authors.affiliation: Institute of Biological Chemistry and Nutrition, University of Hohenheim, Garbenstr. 30, 70599 Stuttgart, Germany.
authors.forename: Christina
authors.initials: C
authors.lastname: Schiborr
authors.forename: Dorothea
authors.initials: D
authors.lastname: Schwamm
authors.forename: Alexa
authors.initials: A
authors.lastname: Kocher
authors.forename: Gerald
authors.initials: G
authors.lastname: Rimbach
authors.forename: Gunter P
authors.initials: GP
authors.lastname: Eckert
authors.forename: Jan
authors.initials: J
authors.lastname: Frank
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology / Q000627:therapeutic use; D003474:Curcumin / Q000494:pharmacology / Q000627:therapeutic use; D016207:Cytokines / Q000235:genetics / Q000378:metabolism; D019803:Glutathione Disulfide / Q000378:metabolism; D051547:Heme Oxygenase-1 / Q000235:genetics; D007249:Inflammation / Q000188:drug therapy / Q000378:metabolism; D008297:Male; D008565:Membrane Proteins / Q000235:genetics; D051379:Mice; D008815:Mice, Inbred Strains; D023421:Models, Animal; D009206:Myocardium / Q000378:metabolism; D051267:NF-E2-Related Factor 2 / Q000378:metabolism; D016328:NF-kappa B / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D064209:Phytochemicals / Q000494:pharmacology / Q000627:therapeutic use; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use; D012333:RNA, Messenger / Q000378:metabolism; D029800:Vaccinium myrtillus
chemical_list: D000975:Antioxidants; D016207:Cytokines; D008565:Membrane Proteins; D051267:NF-E2-Related Factor 2; D016328:NF-kappa B; C495636:Nfe2l2 protein, mouse; D064209:Phytochemicals; D010936:Plant Extracts; D012333:RNA, Messenger; D051547:Heme Oxygenase-1; C496542:Hmox1 protein, mouse; D003474:Curcumin; D019803:Glutathione Disulfide
keywords: Bilberry anthocyanins; Chronic inflammation; Curcumin; Heart; Oxidative stress; Senescence-accelerated mouse prone 8 (SAMP8)