title: Electrokinetic removal of charged species from small sample volumes.
abstract: Cationic or anionic molecules can be selectively injected from an aqueous mixture of charged and neutral species into a capillary by electrophoresis. Under stacking conditions, this selective field enhanced sample injection was studied here as a potential sample preparation technique for the clean-up or purification of microliter samples. As a simple demonstration, cationic small molecules were removed or extracted from a 100μL sample mixture using a commercial capillary electrophoresis instrument. The extraction was monitored using an on-line UV detector. Results from the micellar electrokinetic chromatography analysis of treated and untreated sample revealed significant reduction of cationic species in the treated sample. The technique is essentially a sequence of field enhanced sample injection experiments in capillary electrophoresis.
authors.affiliation: Australian Centre for Research on Separation Science (ACROSS), School of Chemistry, University of Tasmania, Private Bag 75, Hobart, Tasmania 7001, Australia. jquirino@utas.edu.au
authors.forename: Joselito P
authors.initials: JP
authors.lastname: Quirino
mesh_terms: D000838:Anions / Q000737:chemistry; D002412:Cations / Q000737:chemistry; D019075:Electrophoresis, Capillary / Q000379:methods*
chemical_list: D000838:Anions; D002412:Cations