title: Alginate gel microwell arrays using electrodeposition for three-dimensional cell culture.
abstract: In this study, we developed a novel method for fabricating microwell arrays constructed from alginate gels, and the alginate gel microwells were used for three-dimensional (3D) cell culture. The alginate gel microwells were fabricated on a patterned ITO electrode using alginate gel electrodeposition. Embryonic stem (ES) cells or hepatocellular carcinoma cells (HepG2) were cultured in the alginate gel microwells containing 3T3 cells. During the culture, embryoid bodies (EBs) or HepG2 spheroids were successfully fabricated in the alginate gel microwells. The oxygen consumption of the EBs indicated that they were successfully cultured. Liver-specific gene expressions of the HepG2 spheroids apparently increased by performing 3D co-culture in the microwell arrays with 3T3 cells. These results show that the alginate gel microwells are a useful 3D culture system.
authors.affiliation: Graduate School of Environmental Studies, Tohoku University, Sendai 980-8579, Japan.
authors.forename: Fumisato
authors.initials: F
authors.lastname: Ozawa
authors.forename: Kosuke
authors.initials: K
authors.lastname: Ino
authors.forename: Toshiharu
authors.initials: T
authors.lastname: Arai
authors.forename: Javier
authors.initials: J
authors.lastname: Ramón-Azcón
authors.forename: Yasufumi
authors.initials: Y
authors.lastname: Takahashi
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Shiku
authors.forename: Tomokazu
authors.initials: T
authors.lastname: Matsue
mesh_terms: D016475:3T3 Cells; D000464:Alginates / Q000737:chemistry*; D000818:Animals; D018929:Cell Culture Techniques / Q000295:instrumentation*; D018920:Coculture Techniques / Q000295:instrumentation; D004595:Electroplating; D053595:Embryonic Stem Cells / Q000166:cytology; D004867:Equipment Design; D020723:Glucuronic Acid / Q000737:chemistry; D056945:Hep G2 Cells; D006603:Hexuronic Acids / Q000737:chemistry; D006801:Humans; D051379:Mice; D046888:Tissue Array Analysis / Q000295:instrumentation*
chemical_list: D000464:Alginates; D006603:Hexuronic Acids; D020723:Glucuronic Acid