title: Association of IL-10 and CCL5 single nucleotide polymorphisms with tuberculosis in the Sudanese population.
abstract: OBJECTIVE
  To investigate the genetic determinants for developing tuberculosis in Sudan.
  
  
  METHODS
  Case study of 232 patients with tuberculosis and 206 healthy matched controls from Sudan. In the study population, three single nucleotide polymorphisms (SNPs) in the promoter regions of CCL5 and two in the promoter region of IL-10 were genotyped using polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP). These five SNPs influence the expression of these genes.
  
  
  RESULTS
  There were significant differences in allele distribution for CCL5 -28 C/G (rs 2280788) and IL-10 -592 A/C (rs1800872) in patients with tuberculosis compared with healthy controls.
  
  
  CONCLUSIONS
  This indicates that the genotypes obtained for CCL5 and IL-10 are associated with an increased risk of developing active TB.
authors.affiliation: Mycetoma Research Centre, University of Khartoum, Khartoum, Sudan.
authors.forename: Najwa
authors.initials: N
authors.lastname: Mhmoud
authors.affiliation: Mycetoma Research Centre, University of Khartoum, Khartoum, Sudan.
authors.forename: Ahmed
authors.initials: A
authors.lastname: Fahal
authors.affiliation: Erasmus MC, University Medical Centre Rotterdam, Rotterdam, The Netherlands.
authors.forename: W J
authors.initials: WJ
authors.lastname: Wendy van de Sande
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D054458:Amplified Fragment Length Polymorphism Analysis; D016022:Case-Control Studies; D018946:Chemokine CCL5 / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D016753:Interleukin-10 / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D011446:Prospective Studies; D013183:Sputum / Q000382:microbiology; D013397:Sudan; D014376:Tuberculosis / Q000235:genetics* / Q000276:immunology; D055815:Young Adult
chemical_list: C483024:CCL5 protein, human; D018946:Chemokine CCL5; D004269:DNA, Bacterial; D016753:Interleukin-10
keywords: PCR-RFLP; TB; mycobacterium; susceptibility