title: Enhanced primer selection and synthetic amplicon templates optimize high-resolution melting analysis of single-nucleotide polymorphisms in a large population.
abstract: OBJECTIVE
  High-resolution melting (HRM) screening and scanning for single-nucleotide polymorphisms (SNPs) afford the advantages of a quicker, less expensive, and less demanding option compared to other methods for sequence analysis. The evaluation of large populations of patients for multiple SNPs in a high-throughput manner is the next phase in individualized medicine.
  
  
  RESULTS
  We demonstrated that Tm profiles can be generated from gDNA samples that clearly differentiate homozygous ancestral, homozygous SNP, and heterozygous genotypes, while identifying samples of unique outcome without the cumbersome processes of normalization, temperature shifting, and difference plot generation.
  
  
  CONCLUSIONS
  Through expanded primer selection criterion and inclusion of a cloning fragment length double-stranded DNA sequence-specific control template, we are now able to generate additional data via HRM melt domains that are greatly simplified, while considering both the peak melt temperature and profile.
authors.affiliation: Department of Medicine, University of Minnesota Medical School, Minneapolis, MN 55455, USA. bruzz001@umn.edu
authors.forename: Carol M
authors.initials: CM
authors.lastname: Bruzzone
authors.forename: Patrick S
authors.initials: PS
authors.lastname: Tawadros
authors.forename: Lisa A
authors.initials: LA
authors.lastname: Boardman
authors.forename: Clifford J
authors.initials: CJ
authors.lastname: Steer
mesh_terms: D017931:DNA Primers / Q000737:chemistry* / Q000235:genetics; D005260:Female; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods*; D020641:Polymorphism, Single Nucleotide; D012680:Sensitivity and Specificity
chemical_list: D017931:DNA Primers