title: Simple spectrophotometric method for estimation of disodium edetate in topical gel formulations.
abstract: A simple, sensitive, cost-effective and reproducible UV-spectrophotometric method has been developed and validated for the estimation of disodium edetate in topical gel formulations. Solution of disodium edetate reacts with ferric chloride to form complex in 0.1 N HCl giving λmax at 270 nm. Beer's law was obeyed in the concentration range of 5-50 μg/mL (r (2)= 0.9997). The limit of detection and limit of quantitation were found to be 1.190 and 3.608 μg/mL, respectively. The results show that the procedure is accurate, precise, and reproducible (relative standard deviation < 1%), while being simple and less time consuming. The study concluded that the UV-spectrophotometric method could be used for the quantification of disodium edetate in pure form as well as in pharmaceutical formulations.
authors.affiliation: Department of Pharmaceutics, M. M. College of Pharmacy, M. M. University, Mullana, Ambala, Haryana, India.
authors.forename: Sunil
authors.initials: S
authors.lastname: Kamboj
authors.forename: Deepak
authors.initials: D
authors.lastname: Sharma
authors.forename: Anroop B
authors.initials: AB
authors.lastname: Nair
authors.forename: Suman
authors.initials: S
authors.lastname: Kamboj
authors.forename: Rakesh Kumar
authors.initials: RK
authors.lastname: Sharma
authors.forename: Javed
authors.initials: J
authors.lastname: Ali
authors.forename: K
authors.initials: K
authors.lastname: Pramod
authors.forename: S H
authors.initials: SH
authors.lastname: Ansari
keywords: Disodium edetate; UV-spectrophotometric method; method validation