title: Alkaloids from Zephyranthes robusta BAKER and their acetylcholinesterase- and butyrylcholinesterase-inhibitory activity.
abstract: The bulbs of Zephyranthes robusta (Amaryllidaceae) have been extensively analyzed for their chemical constituents, resulting in the isolation of 13 alkaloids. The chemical structures of the isolated compounds were elucidated by mass-spectrometric, and 1D- and 2D-NMR spectroscopic experiments. The complete NMR assignments were achieved for hippeastidine. All isolated alkaloids were evaluated for their erythrocytic acetylcholinesterase and serum butyrylcholinesterase inhibitory activities using the Ellman's method. Significant acetylcholinesterase inhibition activity was exhibited by 8-O-demethylmaritidine (IC₅₀ (HuAChE) 28.0±0.9 μM).
authors.affiliation: ADINACO Research Group, Department of Pharmaceutical Botany and Ecology, Faculty of Pharmacy, Charles University, Heyrovského 1203, CZ-500 05 Hradec Králové, Czech Republic.
authors.forename: Andrea
authors.initials: A
authors.lastname: Kulhánková
authors.forename: Lucie
authors.initials: L
authors.lastname: Cahlíková
authors.forename: Zdeněk
authors.initials: Z
authors.lastname: Novák
authors.forename: Kateřina
authors.initials: K
authors.lastname: Macáková
authors.forename: Jiří
authors.initials: J
authors.lastname: Kuneš
authors.forename: Lubomír
authors.initials: L
authors.lastname: Opletal
mesh_terms: D000110:Acetylcholinesterase / Q000737:chemistry / Q000378:metabolism*; D000470:Alkaloids / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D047151:Amaryllidaceae Alkaloids / Q000737:chemistry; D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification; D002091:Butyrylcholinesterase / Q000737:chemistry / Q000378:metabolism*; D002800:Cholinesterase Inhibitors / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D035264:Flowers / Q000737:chemistry; D019668:Liliaceae / Q000737:chemistry*; D011485:Protein Binding
chemical_list: C459635:8-demethylmaritidine; D000470:Alkaloids; D047151:Amaryllidaceae Alkaloids; D000975:Antioxidants; D002800:Cholinesterase Inhibitors; D000110:Acetylcholinesterase; D002091:Butyrylcholinesterase