title: Bovine papillomavirus type 2 infection and microscopic patterns of urothelial tumors of the urinary bladder in water buffaloes.
abstract: Microscopic patterns of thirty-four urothelial tumors of the urinary bladder of water buffaloes from the Marmara and Black Sea Regions of Turkey are here described. All the animals grazed on lands rich in bracken fern. Histological diagnosis was assessed using morphological parameters recently suggested for the urinary bladder tumors of cattle. Papillary carcinoma was the most common neoplastic lesion (22/34) observed in this study, and low-grade carcinoma was more common (seventeen cases) than high-grade carcinoma (five cases). Papilloma, papillary urothelial neoplasm of low malignant potential (PUNLMP), and invasive carcinomas were less frequently seen. Carcinoma in situ (CIS) was often detected associated with some papillary and invasive carcinomas. De novo (primary) CIS was rare representing 3% of tumors of this series. A peculiar feature of the most urothelial tumors was the presence in the tumor stroma of immune cells anatomically organized in tertiary lymphoid organs (TLOs). Bovine papillomavirus type-2 (PV-2) E5 oncoprotein was detected by molecular and immunohistochemistry procedures. Early protein, E2, and late protein, L1, were also detected by immunohistochemical studies. Morphological and molecular findings show that BPV-2 infection contributes to the development of urothelial bladder carcinogenesis also in water buffaloes.
authors.affiliation: Department of Veterinary Medicine and Animal Productions, Naples University Federico II, Via Delpino 1, 80137 Naples, Italy.
authors.forename: Paola
authors.initials: P
authors.lastname: Maiolino
authors.forename: Ayhan
authors.initials: A
authors.lastname: Ozkul
authors.forename: Aylin
authors.initials: A
authors.lastname: Sepici-Dincel
authors.forename: Franco
authors.initials: F
authors.lastname: Roperto
authors.forename: Gözde
authors.initials: G
authors.lastname: Yücel
authors.forename: Valeria
authors.initials: V
authors.lastname: Russo
authors.forename: Chiara
authors.initials: C
authors.lastname: Urraro
authors.forename: Roberta
authors.initials: R
authors.lastname: Lucà
authors.forename: Marita Georgia
authors.initials: MG
authors.lastname: Riccardi
authors.forename: Manuela
authors.initials: M
authors.lastname: Martano
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Borzacchiello
authors.forename: Iolanda
authors.initials: I
authors.lastname: Esposito
authors.forename: Sante
authors.initials: S
authors.lastname: Roperto
mesh_terms: D000818:Animals; D052688:Bovine papillomavirus 1 / Q000502:physiology*; D002020:Buffaloes / Q000821:virology*; D002278:Carcinoma in Situ / Q000473:pathology / Q000662:veterinary / Q000821:virology; D002417:Cattle; D018076:DNA, Complementary / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics / Q000302:isolation & purification; D007150:Immunohistochemistry; D008214:Lymphocytes / Q000473:pathology; D008297:Male; D030361:Papillomavirus Infections / Q000473:pathology / Q000662:veterinary* / Q000821:virology; D001743:Urinary Bladder / Q000473:pathology* / Q000821:virology*; D019459:Urothelium / Q000473:pathology* / Q000821:virology*
chemical_list: D018076:DNA, Complementary; D004279:DNA, Viral