title: A flow cytometric crossmatch test using endothelial precursor cells isolated from peripheral blood.
abstract: Flow cytometric crossmatch tests provide a donor-specific, cell based method for the detection of alloreactive antibodies in the sera of transplant patients. Conventional crossmatch tests used in solid organ transplantation utilize lymphocytes as target cells to detect the presence of alloreactive HLA antibodies. Isolation of endothelial precursor cells (EPCs) from peripheral blood now allows testing for antibodies reactive with non-HLA endothelial cell antigens.
authors.affiliation: Immunogenetics Laboratory, Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
authors.forename: Annette M
authors.initials: AM
authors.lastname: Jackson
authors.forename: Donna P
authors.initials: DP
authors.lastname: Lucas
authors.forename: Jessica L
authors.initials: JL
authors.lastname: Badders
mesh_terms: D000906:Antibodies / Q000097:blood / Q000276:immunology*; D000918:Antibody Specificity; D001788:Blood Grouping and Crossmatching; D042783:Endothelial Cells / Q000166:cytology / Q000276:immunology; D005434:Flow Cytometry; D006084:Graft Rejection / Q000276:immunology; D006680:HLA Antigens / Q000097:blood / Q000276:immunology*; D006650:Histocompatibility Testing; D006801:Humans; D008214:Lymphocytes / Q000276:immunology*; D008967:Molecular Biology / Q000379:methods; D014019:Tissue Donors; D014180:Transplantation; D014184:Transplantation, Homologous
chemical_list: D000906:Antibodies; D006680:HLA Antigens