title: The antioxidant property of chitosan green tea polyphenols complex induces transglutaminase activation in wound healing.
abstract: The present study examined, for the first time, the in vitro wound healing potential of chitosan green tea polyphenols (CGP) complex based on the activation of transglutaminase (TGM) genes in epidermal morphogenesis. Response surface methodology was applied to determine the optimal processing condition that gave maximum extraction of green tea polyphenols. The antioxidant activity, scavenging ability, and chelating ability were studied and expressed as average EC50 values of CGP and other treatments. In silico analysis and gene coexpression network was subjected to the TGM sequences analysis. The temporal expressions of TGMs were profiled by semi-quantitative reverse transcription (RT)-PCR technology within 10 days after wounding and 2 days postwounding. CGP showed the effectiveness of antioxidant properties, and the observations of histopathological photography showed advanced tissue granulation and epithelialization formation by CGP treatment. In silico and coexpression analysis confirmed the regulation via TGM gene family in dermatological tissues. RT-PCR demonstrated increased levels of TGM1-3 expression induced by CGP treatment. The efficacy of CGP in wound healing based on these results may be ascribed to its antioxidant properties and activation of the expression of TGMs, and is, thus, essential for the facilitated repair of skin injury.
authors.affiliation: Department of Biosystems and Engineering, College of Life Sciences and Biotechnology, Korea University, Seoul, Republic of Korea.
authors.forename: Yao
authors.initials: Y
authors.lastname: Qin
authors.forename: Xing Wei
authors.initials: XW
authors.lastname: Guo
authors.forename: Lei
authors.initials: L
authors.lastname: Li
authors.forename: Hong Wei
authors.initials: HW
authors.lastname: Wang
authors.forename: Wook
authors.initials: W
authors.lastname: Kim
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage* / Q000737:chemistry; D028241:Camellia sinensis / Q000737:chemistry*; D048271:Chitosan / Q000008:administration & dosage* / Q000737:chemistry; D006801:Humans; D008297:Male; D051379:Mice; D010936:Plant Extracts / Q000008:administration & dosage* / Q000737:chemistry; D059808:Polyphenols / Q000008:administration & dosage* / Q000737:chemistry; D011503:Transglutaminases / Q000235:genetics / Q000378:metabolism*; D014945:Wound Healing / Q000187:drug effects*; D014947:Wounds and Injuries / Q000188:drug therapy* / Q000201:enzymology* / Q000235:genetics / Q000503:physiopathology
chemical_list: D000975:Antioxidants; D010936:Plant Extracts; D059808:Polyphenols; D048271:Chitosan; D011503:Transglutaminases