title: Antioxidant properties and neuroprotective effects of isocampneoside II on hydrogen peroxide-induced oxidative injury in PC12 cells.
abstract: Oxidative stress has been considered as a major cause of cell damage in various neurodegenerative disorders. One of the reasonable strategies for delaying the disease's progression is to prevent reactive oxygen species (ROS) mediated cellular injury by dietary or pharmaceutical augmentation of free radical scavengers. Isocampneoside II (ICD) is an active phenylethanoid glycoside isolated from the medicinal hardwood genus Paulownia. This study was designed to explore free radical scavenging potential of ICD in different in vitro systems and its protective role in hydrogen peroxide (H₂O₂)-induced oxidative stress and apoptotic death in cultured rat pheochromocytoma (PC12) cells. The results showed ICD eliminated approximately 80.75% superoxide radical at the concentration of 0.1mg/ml and inhibited metal chelating by 22.07% at 8 mg/ml. Additionally, ICD showed a strong ability on reducing power and provided protection against oxidative protein damage induced by hydroxyl radicals. Pretreatment of PC12 cells with ICD prior to H₂O₂ exposure elevated cell viability, enhanced activity of superoxide dismutase and catalase, and decreased levels of malondialdehyde and intracellular ROS. Furthermore, ICD inhibited cell apoptosis and Bax/Bcl-2 ratio induced by H₂O₂. These findings suggested ICD may be considered as a potential antioxidant agent and should encourage for further research in neurodegenerative diseases.
authors.affiliation: Tianjin Key Laboratory of Pulp & Paper, College of Materials Science & Chemical Engineering, Tianjin University of Science & Technology, Tianjin 300457, PR China. sichli@tust.edu.cn
authors.forename: Chuan-Ling
authors.initials: CL
authors.lastname: Si
authors.forename: Ting
authors.initials: T
authors.lastname: Shen
authors.forename: Yun-Yao
authors.initials: YY
authors.lastname: Jiang
authors.forename: Lei
authors.initials: L
authors.lastname: Wu
authors.forename: Guo-Jing
authors.initials: GJ
authors.lastname: Yu
authors.forename: Xiao-Dan
authors.initials: XD
authors.lastname: Ren
authors.forename: Guang-Hui
authors.initials: GH
authors.lastname: Xu
authors.forename: Wei-Cheng
authors.initials: WC
authors.lastname: Hu
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D051017:Apoptosis Regulatory Proteins / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D002109:Caffeic Acids / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D002614:Chelating Agents / Q000494:pharmacology; D004187:Disaccharides / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects; D006027:Glycosides / Q000494:pharmacology*; D006861:Hydrogen Peroxide / Q000037:antagonists & inhibitors / Q000633:toxicity; D015227:Lipid Peroxidation / Q000187:drug effects*; D009419:Nerve Tissue Proteins / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D009474:Neurons / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D018696:Neuroprotective Agents / Q000494:pharmacology*; D016877:Oxidants / Q000037:antagonists & inhibitors / Q000633:toxicity; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000187:drug effects*; D010088:Oxidoreductases / Q000378:metabolism; D016716:PC12 Cells; D051381:Rats; D017382:Reactive Oxygen Species / Q000037:antagonists & inhibitors / Q000378:metabolism
chemical_list: D000975:Antioxidants; D051017:Apoptosis Regulatory Proteins; D002109:Caffeic Acids; D002614:Chelating Agents; D004187:Disaccharides; D006027:Glycosides; D009419:Nerve Tissue Proteins; D018696:Neuroprotective Agents; D016877:Oxidants; D017382:Reactive Oxygen Species; C587325:isocampneoside II; D006861:Hydrogen Peroxide; D010088:Oxidoreductases
keywords: Antioxidant; Isocampneoside II; Malondialdehyde; Neurodegeneration; Reactive oxygen species