title: In vivo mapping of notch pathway activity in normal and stress hematopoiesis.
abstract: Accumulating evidence suggests that Notch signaling is active at multiple points during hematopoiesis. Until recently, the majority of such studies focused on Notch signaling in lymphocyte differentiation and knowledge of individual Notch receptor roles has been limited due to a paucity of genetic tools available. In this manuscript we generate and describe animal models to identify and fate-map stem and progenitor cells expressing each Notch receptor, delineate Notch pathway activation, and perform in vivo gain- and loss-of-function studies dissecting Notch signaling in early hematopoiesis. These models provide comprehensive genetic maps of lineage-specific Notch receptor expression and activation in hematopoietic stem and progenitor cells. Moreover, they establish a previously unknown role for Notch signaling in the commitment of blood progenitors toward the erythrocytic lineage and link Notch signaling to optimal organismal response to stress erythropoiesis.
authors.affiliation: Howard Hughes Medical Institute and NYU Cancer Institute, New York University School of Medicine, New York, NY 10016, USA.
authors.forename: Philmo
authors.initials: P
authors.lastname: Oh
authors.affiliation: Howard Hughes Medical Institute and NYU Cancer Institute, New York University School of Medicine, New York, NY 10016, USA.
authors.forename: Camille
authors.initials: C
authors.lastname: Lobry
authors.affiliation: Howard Hughes Medical Institute and NYU Cancer Institute, New York University School of Medicine, New York, NY 10016, USA.
authors.forename: Jie
authors.initials: J
authors.lastname: Gao
authors.affiliation: Howard Hughes Medical Institute and NYU Cancer Institute, New York University School of Medicine, New York, NY 10016, USA.
authors.forename: Anastasia
authors.initials: A
authors.lastname: Tikhonova
authors.affiliation: Howard Hughes Medical Institute and NYU Cancer Institute, New York University School of Medicine, New York, NY 10016, USA.
authors.forename: Evangelia
authors.initials: E
authors.lastname: Loizou
authors.affiliation: Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA.
authors.forename: Jan
authors.initials: J
authors.lastname: Manet
authors.affiliation: Department of Molecular, Cell and Developmental Biology, Eli and Edythe Broad Stem Cell Research Center, University of California Los Angeles, 621 Charles E. Young Drive S., Los Angeles, CA 90095, USA.
authors.forename: Ben
authors.initials: B
authors.lastname: van Handel
authors.affiliation: Department of Pathology and NYU Cancer Institute, New York University School of Medicine, New York, NY 10016, USA.
authors.forename: Sherif
authors.initials: S
authors.lastname: Ibrahim
authors.affiliation: Exelixis Inc, South San Francisco, CA 94080, USA.
authors.forename: Jeffrey
authors.initials: J
authors.lastname: Greve
authors.affiliation: Department of Molecular, Cell and Developmental Biology, Eli and Edythe Broad Stem Cell Research Center, University of California Los Angeles, 621 Charles E. Young Drive S., Los Angeles, CA 90095, USA.
authors.forename: Hanna
authors.initials: H
authors.lastname: Mikkola
authors.affiliation: Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA.|Collège de France, 75231 Paris Cedex 05 France.
authors.forename: Spyros
authors.initials: S
authors.lastname: Artavanis-Tsakonas
authors.affiliation: Howard Hughes Medical Institute and NYU Cancer Institute, New York University School of Medicine, New York, NY 10016, USA.
authors.forename: Iannis
authors.initials: I
authors.lastname: Aifantis
mesh_terms: D000818:Animals; D051792:Basic Helix-Loop-Helix Proteins / Q000235:genetics / Q000378:metabolism; D002454:Cell Differentiation / Q000235:genetics; D002455:Cell Division; D019070:Cell Lineage / Q000235:genetics; D041905:Erythroid Cells / Q000166:cytology / Q000378:metabolism; D005333:Fetus / Q000166:cytology; D020869:Gene Expression Profiling; D017930:Genes, Reporter; D006410:Hematopoiesis; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008954:Models, Biological; D051881:Receptor, Notch1 / Q000378:metabolism*; D051883:Receptor, Notch2 / Q000378:metabolism*; D015398:Signal Transduction; D013312:Stress, Physiological / Q000235:genetics; D000072056:Transcription Factor HES-1; D014158:Transcription, Genetic
chemical_list: D051792:Basic Helix-Loop-Helix Proteins; D018398:Homeodomain Proteins; D051881:Receptor, Notch1; D051883:Receptor, Notch2; D000072056:Transcription Factor HES-1; C489734:Hes1 protein, mouse