title: SIRT3 expression in hepatocellular carcinoma and its impact on proliferation and invasion of hepatoma cells.
abstract: OBJECTIVE
  To observe expression of SIRT3 in normal liver tissue, cirrhotic tissue and hepatocellular carcinoma (HCC) tissues, and to explore the significance of SIRT3 in primary HCC.
  
  
  METHODS
  SIRT3 expression was detected in 10 normal cases, 30 cases with, 30 HCC cases by immunohistochemical and Western-blotting method.
  
  
  RESULTS
  Immunohistochemical assay showed that the SIRT3 positive expression rates were 100.0% (10/10), 96.7% (29/30) and 60.0% (18/30), respectively in normal group, paracancer group and HCC group. And the SIRT3 expression in HCC group was significantly lower than in normal group and paracancer group (P<0.05). Western-blotting showed the SIRT3 expression in cancer tissue was 0.29±0.07, significantly lower than that in paracancer group and normal group (P<0.05). SIRT3 expression was related to the differentiation degree and portal vein tumor thrombus (P<0.05).
  
  
  CONCLUSIONS
  Abnormal expression of SIRT3 is closely related to the biological behavior of primary HCC.
authors.affiliation: First Affiliated Hospital of Soochow University, Suzhou, China. Zhangbo210326079@163.com
authors.forename: Bo
authors.initials: B
authors.lastname: Zhang
authors.forename: Lei
authors.initials: L
authors.lastname: Qin
authors.forename: Cui-Jie
authors.initials: CJ
authors.lastname: Zhou
authors.forename: Ye-Lu
authors.initials: YL
authors.lastname: Liu
authors.forename: Hai-Xin
authors.initials: HX
authors.lastname: Qian
authors.forename: Song-Bing
authors.initials: SB
authors.lastname: He
mesh_terms: D000328:Adult; D000368:Aged; D006528:Carcinoma, Hepatocellular / Q000473:pathology*; D049109:Cell Proliferation; D005260:Female; D022781:Hepatocytes / Q000502:physiology*; D006801:Humans; D007150:Immunohistochemistry; D008099:Liver / Q000473:pathology; D008103:Liver Cirrhosis / Q000473:pathology; D008113:Liver Neoplasms / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D009361:Neoplasm Invasiveness / Q000473:pathology*; D056566:Sirtuin 3 / Q000096:biosynthesis*
chemical_list: C466093:SIRT3 protein, human; D056566:Sirtuin 3