title: Gender dimorphism in regulation of plasma proteins in streptozotocin-induced diabetic rats.
abstract: In the present study, we examined differentially regulated plasma proteins between healthy control and streptozotocin (STZ)-induced male and female diabetic rats by 2DE-based proteomic analysis. Animal experiments revealed that significantly lower plasma insulin levels were observed in female diabetic rats, consequently resulting in higher blood glucose levels in female diabetic rats. Importantly, plasma levels of sex hormones were significantly altered in a gender-dependent manner before and after STZ treatment. Results of the animal experiment indicated the existence of sexual dimorphism in the regulation of plasma proteins between healthy control and diabetic rats. Plasma proteome analysis enabled us to identify a total of 38 proteins showing sexual dimorphic regulation patterns. In addition, for the first time, we identified several differentially regulated plasma proteins between healthy control and diabetic rats, including apolipoprotein E, fetuin B, α-1-acid glycoprotein, β-2-glycoprotein 1, 3-hydroxyanthranilate 3,4-dioxygenase, and serum amyloid P-component. To the best of our knowledge, this is the first proteomic approach to address sexual dimorphism in diabetic animals. These proteomic data on gender-dimorphic regulation of plasma proteins provide valuable information that can be used for evidence-based gender-specific clinical treatment of diabetes.
authors.affiliation: Department of Biotechnology, Daegu University, Kyungsan, Republic of Korea.
authors.forename: Jung-Won
authors.initials: JW
authors.lastname: Choi
authors.forename: Kanikkai Raja
authors.initials: KR
authors.lastname: Aseer
authors.forename: Harmesh N
authors.initials: HN
authors.lastname: Chaudhari
authors.forename: Rajib
authors.initials: R
authors.lastname: Mukherjee
authors.forename: Minji
authors.initials: M
authors.lastname: Choi
authors.forename: Jong Won
authors.initials: JW
authors.lastname: Yun
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D001798:Blood Proteins / Q000032:analysis* / Q000737:chemistry / Q000145:classification; D015153:Blotting, Western; D003921:Diabetes Mellitus, Experimental / Q000097:blood* / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D008297:Male; D010641:Phenotype; D020543:Proteome / Q000032:analysis* / Q000737:chemistry; D040901:Proteomics; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D012727:Sex Characteristics; D013311:Streptozocin
chemical_list: D001798:Blood Proteins; D020543:Proteome; D013311:Streptozocin
keywords: 2DE; Diabetes; Gender differences; Plasma proteome; Streptozotocin