title: Two family 11 xylanases from Achaetomium sp. Xz-8 with high catalytic efficiency and application potentials in the brewing industry.
abstract: This study identified two family-11 xylanase genes (xynC81 and xynC83) in Achaetomium sp. Xz-8, a thermophilic strain from a desert area with substantial xylanase activity, and successfully expressed them in Pichia pastoris . Their deduced amino acid sequences showed the highest identity of ≤90% to known fungal xylanases and of ≤62% with each other. The purified recombinant xylanases showed optimal activities at pH 5.5 and 60-65 °C and exhibited stability over pH 5.0-10.0 and temperatures at 55 °C and below. XynC81 had high catalytic efficiency (6082 mL/s/mg), and XynC83 was favorable for xylooligosaccharide production. Under simulated mashing conditions, combination of XynC83 and a commercial β-glucanase improved the filtration rate by 34.76%, which is much better than that of Novozymes Ultraflo (20.71%). XynC81 and XynC83 had a synergistic effect on viscosity reduction (7.08%), which is comparable with that of Ultraflo (8.47%). Thus, XynC81 and XynC83 represent good candidates for application in the brewing industry.
authors.affiliation: Key Laboratory for Feed Biotechnology of the Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, People's Republic of China.
authors.forename: Liang
authors.initials: L
authors.lastname: Zhao
authors.forename: Kun
authors.initials: K
authors.lastname: Meng
authors.forename: Yingguo
authors.initials: Y
authors.lastname: Bai
authors.forename: Pengjun
authors.initials: P
authors.lastname: Shi
authors.forename: Huoqing
authors.initials: H
authors.lastname: Huang
authors.forename: Huiying
authors.initials: H
authors.lastname: Luo
authors.forename: Yaru
authors.initials: Y
authors.lastname: Wang
authors.forename: Peilong
authors.initials: P
authors.lastname: Yang
authors.forename: Wei
authors.initials: W
authors.lastname: Song
authors.forename: Bin
authors.initials: B
authors.lastname: Yao
mesh_terms: D000595:Amino Acid Sequence; D001515:Beer; D002384:Catalysis; D003001:Cloning, Molecular; D043364:Endo-1,4-beta Xylanases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D005511:Food Handling / Q000379:methods; D015870:Gene Expression; D007700:Kinetics; D008958:Models, Molecular; D010843:Pichia / Q000201:enzymology / Q000235:genetics; D011994:Recombinant Proteins / Q000378:metabolism; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D020037:Sordariales / Q000201:enzymology* / Q000235:genetics; D013379:Substrate Specificity
chemical_list: D011994:Recombinant Proteins; D043364:Endo-1,4-beta Xylanases