title: A specific polymerase chain reaction method to identify Stenotrophomonas maltophilia.
abstract: Stenotrophomonas maltophilia is a multidrug-resistant nosocomial pathogen that is difficult to identify unequivocally using current methods. Accordingly, because the presence of this microorganism in a patient may directly determine the antimicrobial treatment, conventional polymerase chain reaction (PCR) and real-time PCR assays targeting 23S rRNA were developed for the specific identification of S. maltophilia. The PCR protocol showed high specificity when tested against other species of Stenotrophomonas, non-fermentative Gram-negative bacilli and 100 clinical isolates of S. maltophilia previously identified using the Vitek system.
authors.affiliation: Pontifícia Universidade Católica do Rio Grande do Su, Faculdade de Biociências, Departamento de Biologia Celular e Molecular, Laboratório de Imunologia e Microbiologia, Porto Alegre, RS, Brasil.
authors.forename: Stephanie Wagner
authors.initials: SW
authors.lastname: Gallo
authors.forename: Patrícia Locosque
authors.initials: PL
authors.lastname: Ramos
authors.forename: Carlos Alexandre Sanchez
authors.initials: CA
authors.lastname: Ferreira
authors.forename: Sílvia Dias de
authors.initials: SD
authors.lastname: Oliveira
mesh_terms: D015373:Bacterial Typing Techniques; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D012338:RNA, Ribosomal, 23S / Q000235:genetics*; D020615:Stenotrophomonas maltophilia / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D012338:RNA, Ribosomal, 23S