title: WUSCHEL-responsive At5g65480 interacts with CLAVATA components in vitro and in transient expression.
abstract: The CLAVATA (CLV) signaling pathway is essential for shoot meristem homeostasis in Arabidopsis. CLV acts to limit the expression domain of the stem cell-promoting gene WUSCHEL (WUS). The closely related receptor-kinases CLV1 and BAM1 are key components in this pathway; however, the downstream factors that link the receptors to WUS regulation are poorly understood. The Arabidopsis gene At5g65480 was recently identified as a direct transcriptional target up-regulated by WUS. We have independently identified this gene which we term CCI1 as a CLV1 and BAM1 interacting protein in vitro and in transient expression. CCI1 has phosphatidylinositide-binding activity in vitro and localizes to the plasma membrane in transient expression. Furthermore, CLV signaling components and CCI1 both partition to detergent-resistant membrane microdomains characterized as lipid rafts.
authors.affiliation: Department of Molecular, Cellular and Developmental Biology, University of Michigan, Ann Arbor, Michigan, United States of America.
authors.forename: Lindsey A
authors.initials: LA
authors.lastname: Gish
authors.forename: Jennifer M
authors.initials: JM
authors.lastname: Gagne
authors.forename: Linqu
authors.initials: L
authors.lastname: Han
authors.forename: Brody J
authors.initials: BJ
authors.lastname: Deyoung
authors.forename: Steven E
authors.initials: SE
authors.lastname: Clark
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant / Q000235:genetics / Q000502:physiology; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction / Q000235:genetics / Q000502:physiology
chemical_list: D029681:Arabidopsis Proteins; D018398:Homeodomain Proteins; C116421:WUSCHEL protein, Arabidopsis