title: Novel functionalized fluorescent polymeric nanoparticles for immobilization of biomolecules.
abstract: Novel, size controlled fluorescent polymeric nanoparticles (FPNP) were synthesized having acetoacetoxy functionality on the surface for immobilization of biomolecules which can be utilized as biomarkers and labels in fluoroimmunoassays. Core-shell nanoparticles of poly(styrene, St-methyl methacrylate, MMA-acetoacetoxy ethyl methacrylate, AAEM), stabilized by various concentrations of surfactant, sodium lauryl sulphate (SLS), were obtained by facile miniemulsion co-polymerization encapsulated with pyrene molecules in their hydrophobic core. Analytical, spectroscopic and imaging characterization techniques revealed the formation of stable, monodisperse, spherical nano sized particles exhibiting high luminescence properties. Particles with 1% SLS (S1) showed good dispersion stability and fluorescence intensity and were chosen as ideal candidates for further immobilization studies. Steady state fluorescence studies showed 10 times higher fluorescence intensity of S1 nanoparticles than that of pyrene solution in solvent-toluene at the same concentration. Environmental factors such as pH, ionic strength and time were found to have no effect on fluorescence intensity of FPNPs. Surface β-di-ketone groups were utilized for the covalent immobilization of enzyme conjugated antibodies without any activation or pre-treatment of nanoparticles.
authors.affiliation: Centre for Biomedical Engineering, Indian Institute of Technology-Delhi, New Delhi-16, India. swatijain.iitd@gmail.com
authors.forename: Swati
authors.initials: S
authors.lastname: Jain
authors.forename: Sruti
authors.initials: S
authors.lastname: Chattopadhyay
authors.forename: Richa
authors.initials: R
authors.lastname: Jackeray
authors.forename: C K V Zainul
authors.initials: CK
authors.lastname: Abid
authors.forename: Harpal
authors.initials: H
authors.lastname: Singh
mesh_terms: D000818:Animals; D000906:Antibodies / Q000737:chemistry*; D004800:Enzymes, Immobilized / Q000737:chemistry*; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry*; D011109:Polymethacrylic Acids / Q000737:chemistry; D011137:Polystyrenes / Q000737:chemistry; D011721:Pyrenes / Q000737:chemistry*; D011817:Rabbits; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry
chemical_list: D000906:Antibodies; D004800:Enzymes, Immobilized; D005456:Fluorescent Dyes; D011109:Polymethacrylic Acids; D011137:Polystyrenes; D011721:Pyrenes; D012967:Sodium Dodecyl Sulfate; C030984:pyrene