title: Fast analysis of triterpenoids in Ganoderma lucidum spores by ultra-performance liquid chromatography coupled with triple quadrupole mass spectrometry.
abstract: A rapid and reliable method was established for simultaneous determination of main triterpenoids in Ganoderma lucidum spores using ultra-high-performance liquid chromatography coupled with triple quadrupole mass spectrometry (UPLC-TQ-MS). The established method was validated in terms of linearity, sensitivity, precision, accuracy and stability, and was successfully applied to determine the contents of 10 main triterpenoids in different batches of G. lucidum spores. The analysis results showed that moderate levels of triterpenoids were found in G. lucidum spores. In addition, a MS full scan with a daughter ion scan experiment was performed to identify the potential derivatives of triterpenoids present in G. lucidum spores. As a result, a total of 22 triterpenoids from different G. lucidum spores were unequivocally or tentatively identified via comparisons with authentic standards and literatures. This method provides both qualitative and quantitative results without the need for repetitive UPLC-MS analyses, thereby increasing efficiency and productivity, making it suitable for high-throughput applications.
authors.affiliation: Shanghai University of Traditional Chinese Medicines, Shanghai, 201203, China; Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, 610041, China.
authors.forename: Zhou
authors.initials: Z
authors.lastname: Yan
authors.forename: Bing
authors.initials: B
authors.lastname: Xia
authors.forename: Ming Hua
authors.initials: MH
authors.lastname: Qiu
authors.forename: Ding
authors.initials: D
authors.lastname: Li Sheng
authors.forename: Hong Xi
authors.initials: HX
authors.lastname: Xu
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000191:economics / Q000379:methods*; D057230:Limit of Detection; D013058:Mass Spectrometry / Q000191:economics / Q000379:methods; D020940:Reishi / Q000737:chemistry*; D013172:Spores, Fungal / Q000737:chemistry*; D013997:Time Factors; D014315:Triterpenes / Q000032:analysis*
chemical_list: D014315:Triterpenes
keywords: Ganoderma lucidum spores; daughter ion scan experiment; multiple reaction monitoring; triterpenoid