title: An interpenetrating HA/G/CS biomimic hydrogel via Diels-Alder click chemistry for cartilage tissue engineering.
abstract: In order to mimic the natural cartilage extracellular matrix, a novel biological degradable interpenetrating network hydrogel was synthesized from the gelatin (G), hyaluronic acid (HA) and chondroitin sulfate (CS) by Diels-Alder "click" chemistry. HA was modified with furylamine and G was modified with furancarboxylic acid respectively. (1)H NMR spectra and elemental analysis showed that the substitution degrees of HA-furan and G-furan were 71.5% and 44.5%. Then the hydrogels were finally synthesized by cross-linking furan-modified HA and G derivatives with dimaleimide poly(ethylene glycol) (MAL-PEG-MAL). The mechanical and degradation properties of the hydrogels could be tuned simply through varying the molar ratio between furan and maleimide. Rheological, mechanical and degradation studies demonstrated that the Diels-Alder "click" chemistry is an efficient method for preparing high performance biological interpenetrating hydrogels. This biomimic hydrogel with improved mechanical properties could have great potential applications in cartilage tissue engineering.
authors.affiliation: School of Materials Science and Engendering, South China University of Technology, Guangzhou 510641, PR China.
authors.forename: Feng
authors.initials: F
authors.lastname: Yu
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Cao
authors.forename: Lei
authors.initials: L
authors.lastname: Zeng
authors.forename: Qing
authors.initials: Q
authors.lastname: Zhang
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Chen
mesh_terms: D002356:Cartilage / Q000502:physiology; D002809:Chondroitin Sulfates / Q000737:chemistry*; D057930:Click Chemistry; D061565:Cycloaddition Reaction; D005663:Furans / Q000737:chemistry; D005780:Gelatin / Q000737:chemistry*; D006820:Hyaluronic Acid / Q000737:chemistry*; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry*; D008301:Maleimides / Q000737:chemistry; D011092:Polyethylene Glycols / Q000737:chemistry; D012038:Regeneration; D023822:Tissue Engineering
chemical_list: D005663:Furans; D008301:Maleimides; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D011092:Polyethylene Glycols; D005780:Gelatin; D006820:Hyaluronic Acid; D002809:Chondroitin Sulfates