title: Exosomes of BV-2 cells induced by alpha-synuclein: important mediator of neurodegeneration in PD.
abstract: Parkinson's disease (PD) is a progressive neurodegenerative disease. Alpha-synuclein aggregation, which can activate microglia to enhance its dopaminergic neurotoxicity, plays a central role in the progression of PD. However the mechanism is still unclear. To investigate how alpha-synuclein affects the neuron, exosomes were derived from alpha-synuclein treated mouse microglia cell line BV-2 cells by differential centrifugation and ultracentrifugation. We found that alpha-synuclein can induce an increase of exosomal secretion by microglia. These activated exosomes expressed a high level of MHC class II molecules and membrane TNF-α. In addition, the activated exosomes cause increased apoptosis. Exosomes secreted from activated microglias might be important mediator of alpha-synuclein-induced neurodegeneration in PD.
authors.affiliation: Department of Neurosurgery, Tangdu Hospital, The Fourth Military Medical University, Xi'an, PR China.
authors.forename: Chongwang
authors.initials: C
authors.lastname: Chang
authors.forename: Hongjuan
authors.initials: H
authors.lastname: Lang
authors.forename: Ning
authors.initials: N
authors.lastname: Geng
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.forename: Nan
authors.initials: N
authors.lastname: Li
authors.forename: Xuelian
authors.initials: X
authors.lastname: Wang
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002460:Cell Line; D055354:Exosomes / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D017628:Microglia / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology*; D009410:Nerve Degeneration / Q000378:metabolism*; D010300:Parkinson Disease / Q000378:metabolism* / Q000473:pathology; D051844:alpha-Synuclein / Q000378:metabolism* / Q000494:pharmacology*
chemical_list: D051844:alpha-Synuclein
keywords: APCs; Alpha-synuclein; CM; Exosome; FWB; LBs; Lewy bodies; Microglia; PD; Parkinson's disease; TACE; TNF-α converting enzyme; antigen-presenting cells; culture medium; flow cytometry wash buffer