title: Advances in lectin microarray technology: optimized protocols for piezoelectric print conditions.
abstract: Lectin microarray technology has been used to profile the glycosylation of a multitude of biological and clinical samples, leading to new clinical biomarkers and advances in glycobiology. Lectin microarrays, which include >90 plant lectins, recombinant lectins, and selected antibodies, are used to profile N-linked, O-linked, and glycolipid glycans. The specificity and depth of glycan profiling depends upon the carbohydrate-binding proteins arrayed. The current set targets mammalian carbohydrates including fucose, high mannose, branched and complex N-linked, α- and β-galactose and GalNAc, α-2,3- and α-2,6-sialic acid, LacNAc, and Lewis X epitopes. Previous protocols have described the use of a contact microarray printer for lectin microarray production. Here, an updated protocol that uses a non-contact, piezoelectric printer, which leads to increased lectin activity on the array, is presented. Optimization of print and sample hybridization conditions and methods of analysis are discussed.
authors.affiliation: Department of Chemistry, Biomedical Chemistry Institute, New York University, New York, USA.
authors.forename: Kanoelani T
authors.initials: KT
authors.lastname: Pilobello
authors.forename: Praveen
authors.initials: P
authors.lastname: Agrawal
authors.forename: Richard
authors.initials: R
authors.lastname: Rouse
authors.forename: Lara K
authors.initials: LK
authors.lastname: Mahal
mesh_terms: D000818:Animals; D002241:Carbohydrates / Q000032:analysis; D037102:Lectins / Q000737:chemistry*; D040081:Protein Array Analysis / Q000295:instrumentation / Q000379:methods*; D012107:Research Design; D013194:Staining and Labeling
chemical_list: D002241:Carbohydrates; D037102:Lectins
keywords: Nano‐Plotter; carbohydrate analysis; glycomics; lectin microarray; piezoelectric