title: Evaluation of the Pan Influenza detection kit utilizing the PLEX-ID and influenza samples from the 2011 respiratory season.
abstract: A comparison study was performed between the PLEX-ID and the CDC RT-PCR method for the detection and identification of Influenza A viruses using nasopharyngeal samples (N=75) collected between January and May 2011. Overall agreement was 89.3% (67/75 kappa=0.57 95% CI 0.3-0.89). Positive percent agreement was 92.3% (60/65); negative percent agreement was 70% (7/10). H1N1 pdm09 identified: 42.6% (32/75) and 54.7% (41/75) by PLEX-ID and CDC RT-PCR, respectively. H3N2 identified: 29.3% (22/75) and 32% (24/75) of samples by PLEX-ID and CDC RT-PCR, respectively. Negatives identified: 16% (12/75) and 13.3% (10/75), by PLEX-ID and CDC RT-PCR respectively. For influenza viruses identified as H1N1 pdm09, Influenza A virus A/NEW YORK/15/2009(H1N1 pdm09) was the most prevalent genotype at 50% (16/32), followed by A/CALIFORNIA/05/2009(H1N1 pdm09) at 18.2% (6/32). Updated assay plates containing additional primers designed for H1N1 pdm09 HA and NA genes were utilized for this evaluation. Among H1N1 pdm09 samples, the HA gene was conserved in 96.9% (31/32) of samples. The NA gene was conserved in 96.9% (31/32).
authors.affiliation: The Johns Hopkins University School of Medicine, Division of Infectious Diseases, 855 North Wolfe Street, Rangos Building, Room 530, Baltimore, MD 21205, United States. lmurill2@jhmi.edu
authors.forename: Luis
authors.initials: L
authors.lastname: A Murillo
authors.forename: Justin
authors.initials: J
authors.lastname: Hardick
authors.forename: Kevin
authors.initials: K
authors.lastname: Jeng
authors.forename: Charlotte A
authors.initials: CA
authors.lastname: Gaydos
mesh_terms: D005838:Genotype; D006801:Humans; D009980:Influenza A virus / Q000235:genetics / Q000302:isolation & purification*; D007251:Influenza, Human / Q000175:diagnosis* / Q000821:virology; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D009305:Nasopharynx / Q000821:virology; D014773:Virology / Q000379:methods*
keywords: Genotyping; H1N1 pdm09; Influenza A virus; PLEX-ID