title: Sensitive and accurate estimation of losartan potassium formulation by high-performance thin-layer chromatography.
abstract: OBJECTIVE
  To develop a simple, sensitive, cost-effective and reproducible UV-spectrophotometric method and validate for the estimation of disodium edetate in topical gel formulations.
  
  
  METHODS
  Solution of disodium edetate reacts with ferric chloride to form complex in 0.1 N HCl giving λmax at 270 nm. Beer's law was obeyed in the concentration range of 5-50 μg/mL (r (2) = 0.9997).
  
  
  RESULTS
  The limit of detection and limit of quantitation were found to be 1.190 and 3.608 μg/mL, respectively. The results show that the procedure is accurate, precise, and reproducible (relative standard deviation < 1%), while being simple and less time consuming.
  
  
  CONCLUSIONS
  The study concluded that the UV-spectrophotometric method could be used for the quantification of disodium edetate in pure form as well as in pharmaceutical formulations.
authors.affiliation: Department of Pharmaceutical Analysis, JSS College of Pharmacy, Rocklands-Ooty, Tamilnadu, India.
authors.forename: Muralidharan
authors.initials: M
authors.lastname: Selvadurai
authors.forename: Subramaniya Nainar
authors.initials: SN
authors.lastname: Meyyanathan
keywords: Estimation; high-performance thin-layer chromatographic; losartan potassium; tablets