title: Isolation and determination of deoxynivalenol by reversed-phase high-pressure liquid chromatography.
abstract: Deoxynivalenol (DON) is a mycotoxin produced by food contamination. It is a pharmacologically active compound that acts on the serotonin receptor, leading to several neuroendocrine and hematological disorders. In this article we describe a simple, accurate, and sensitive method for the quantification of DON. DON was quantified using a Phenomenex(®) ODS analytical C18 column (150 mm × 46 mm, 5 μm) with a mobile phase composed of mixture of water-acetonitrile-methanol (5:4:1, v/v/v) at a flow rate of 1.5 ml/min and at 254 nm in an ultraviolet (UV) detector The method has been validated with isolated samples of DON and provides a tool for the control of substandard and counterfeit commercial food products.
authors.affiliation: Division of Biotechnology, Defense Research Laboratory, Post Bag No: 2, Tezpur-784 001, India.
authors.forename: Vikas Kumar
authors.initials: VK
authors.lastname: Gupta
authors.forename: Pronobesh
authors.initials: P
authors.lastname: Chattopadhyay
authors.forename: Mohan Ch
authors.initials: MCh
authors.lastname: Kalita
authors.forename: Asshwani Kumar
authors.initials: AK
authors.lastname: Chaurasia
authors.forename: Hemanta Kumar
authors.initials: HK
authors.lastname: Gogoi
authors.forename: Lokendra
authors.initials: L
authors.lastname: Singh
keywords: Deoxynivalenol; Fusarium graminearum; HPLC; mycotoxins