title: GammaProteobacteria as a potential bioindicator of a multiple contamination by polycyclic aromatic hydrocarbons (PAHs) in agricultural soils.
abstract: The impact of a multiple contamination by polycyclic aromatic hydrocarbons (PAHs) was studied on permanent grassland soil, historically presenting low contamination (i.e. less than 1 mg kg(-1)). Soil microcosms were spiked at 300 mg kg(-1) with either single or a mixture of seven PAHs. While total dissipation of the phenanthrene was reached in under 90 days, only 60% of the PAH mixture were dissipated after 90 days. Interestingly, after 30 days, the abundance of the GammaProteobacteria class (assessed by qPCR) become significantly higher in microcosms spiked with the PAH mixture. In addition, the specific abundance of the cultivable Pseudomonas spp., which belong to the GammaProteobacteria class, increased earlier and transiently (after 8 days) in the microcosms spiked with the PAH mixture. Consequently, we propose to use the GammaProteobacteria as a bioindicator to detect the impact on the bacterial community of a multiple contamination by PAHs in agricultural soils.
authors.affiliation: Université de Rouen, Laboratoire de Microbiologie Signaux et Microenvironnement, EA 4312, 76821 Mont Saint Aignan, France.
authors.forename: Maïté
authors.initials: M
authors.lastname: Niepceron
authors.forename: Fabrice
authors.initials: F
authors.lastname: Martin-Laurent
authors.forename: Marc
authors.initials: M
authors.lastname: Crampon
authors.forename: Florence
authors.initials: F
authors.lastname: Portet-Koltalo
authors.forename: Marthe
authors.initials: M
authors.lastname: Akpa-Vinceslas
authors.forename: Marc
authors.initials: M
authors.lastname: Legras
authors.forename: David
authors.initials: D
authors.lastname: Bru
authors.forename: Fabrice
authors.initials: F
authors.lastname: Bureau
authors.forename: Josselin
authors.initials: J
authors.lastname: Bodilis
mesh_terms: D000383:Agriculture; D004784:Environmental Monitoring / Q000379:methods*; D020563:Gammaproteobacteria / Q000502:physiology*; D011084:Polycyclic Aromatic Hydrocarbons / Q000032:analysis* / Q000378:metabolism; D012987:Soil / Q000737:chemistry*; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000032:analysis* / Q000378:metabolism
chemical_list: D011084:Polycyclic Aromatic Hydrocarbons; D012987:Soil; D012989:Soil Pollutants
keywords: Agricultural soil; Bioindicator; Polycyclic aromatic hydrocarbon; Pseudomonas; qPCR