title: Hydrophobic cryogels for DNA adsorption: effect of embedding of monosize microbeads into cryogel network on their adsorptive performances.
abstract: As alternative hydrophobic adsorbent for DNA adsorption, supermacroporous cryogel disks were synthesized via free radical polymerization. In this study, we have prepared two kinds of cryogel disks: (i) poly(2-hydroxyethyl methacrylate-N-methacryloyl-l-tryptophan) [p(HEMA-MATrp)] cryogel containing specific hydrophobic ligand MATrp; and (ii) monosize p(HEMA-MATrp) particles synthesized via suspension polymerization embedded into p(HEMA) cryogel structure to obtain p(HEMA-MATrp)/p(HEMA) composite cryogel disks. These cryogel disks containing hydrophobic functional group were characterized via swelling studies, Fourier transform infrared spectroscopy, elemental analysis, surface area measurements and scanning electron microscopy. DNA adsorption onto both p(HEMA-MATrp) cryogel and p(HEMA-MATrp)/p(HEMA) composite cryogels was investigated. Maximum adsorption of DNA on p(HEMA-MATrp) cryogel was found to be 15 mg/g polymer. Otherwise, p(HEMA-MATrp)/p(HEMA) composite cryogels significantly increased the DNA adsorption capacity to 38 mg/g polymer. Composite cryogels could be used repeatedly without significant loss on adsorption capacity after 10 repetitive adsorption-desorption cycles.
authors.affiliation: Hacettepe University, Chemistry Department, Ankara, Turkey.
authors.forename: M
authors.initials: M
authors.lastname: Emin Çorman
authors.forename: Nilay
authors.initials: N
authors.lastname: Bereli
authors.forename: Serpil
authors.initials: S
authors.lastname: Özkara
authors.forename: Lokman
authors.initials: L
authors.lastname: Uzun
authors.forename: Adil
authors.initials: A
authors.lastname: Denizli
mesh_terms: D000327:Adsorption; D059985:Cryogels / Q000737:chemistry*; D004247:DNA / Q000302:isolation & purification*; D057927:Hydrophobic and Hydrophilic Interactions; D011102:Polyhydroxyethyl Methacrylate / Q000737:chemistry*; D014364:Tryptophan / Q000031:analogs & derivatives*
chemical_list: D059985:Cryogels; D011102:Polyhydroxyethyl Methacrylate; D014364:Tryptophan; D004247:DNA
keywords: DNA; cryogel disk; hydrophobic interaction chromatography; monosize microbead embedding; polymeric composites