title: Single embryo-coupled gate field effect transistor for elective single embryo transfer.
abstract: In this study, we have proposed and demonstrated experimentally a novel monitoring device of single mouse embryo activity after in vitro fertilization (IVF) using a semiconductor-based field effect transistor (FET). The FET biosensor realized to detect it noninvasively, quantitatively, and continuously by change of hydrogen ions with positive charges, which were induced by dissolved carbon dioxide due to cellular respiration activity during cleavage. The electrical signal of FET biosensor should become an effective indication to evaluate objectively single embryo activity as its morphology is observed subjectively after IVF. The platform based on the FET biosensor will contribute to promote elective single embryo transfer (eSET) in human assisted reproductive technology (ART).
authors.affiliation: Department of Materials Engineering, School of Engineering, The University of Tokyo, Bunkyo-ku, Tokyo, Japan. sakata@biofet.t.u-tokyo.ac.jp
authors.forename: Toshiya
authors.initials: T
authors.lastname: Sakata
authors.forename: Akiko
authors.initials: A
authors.lastname: Saito
authors.forename: Jinji
authors.initials: J
authors.lastname: Mizuno
authors.forename: Haruyo
authors.initials: H
authors.lastname: Sugimoto
authors.forename: Kaori
authors.initials: K
authors.lastname: Noguchi
authors.forename: Eiko
authors.initials: E
authors.lastname: Kikuchi
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Inui
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D046149:Embryo Culture Techniques / Q000295:instrumentation / Q000379:methods; D005260:Female; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D011247:Pregnancy; D056826:Single Embryo Transfer / Q000295:instrumentation / Q000379:methods*; D014173:Transistors, Electronic