title: Biocompatibility assessment of porous chitosan-Nafion and chitosan-PTFE composites in vivo.
abstract: Chitosan (CS) is widely used as a scaffold material in tissue engineering. The objective of this study was to test whether porous chitosan membrane (PCSM) coating for Nafion used in implantable sensor reduced fibrous capsule (FC) density and promoted superior vascularization compared with PCSM coating for polytetrafluoroethylene (PTFE). PCSM was fabricated with solvent casting/particulate leaching method using silica gel as porogen and characterized in vitro. Then, PCSM-Nafion and PCSM-PTFE composites were assembled with hydrated PCSM and implanted subcutaneously in rats. The histological analysis was performed in comparison with Nafion and PTFE. Implants were explanted 35, 65, and 100 days after the implantation. Histological assessments indicated that both composites achieved presumed effects of porous coatings on decreasing collagen deposition and promoting angiogenesis. PCSM-PTFE exerted higher collagen deposition by area ratio, both within and outside, compared with that of PCSM-Nafion. Angiogenesis within and outside the PCSM-Nafion both increased over time, but that of the PCSM-PTFE within decreased.
authors.affiliation: Department of Radiation Oncology, State Key Laboratory of Oncology in South China, Sun Yat-sen University Cancer Center, SYSUCC, Guangzhou, China.
authors.forename: Bo-Ji
authors.initials: BJ
authors.lastname: Liu
authors.forename: Li-Nan
authors.initials: LN
authors.lastname: Ma
authors.forename: Juan
authors.initials: J
authors.lastname: Su
authors.forename: Wei-Wei
authors.initials: WW
authors.lastname: Jing
authors.forename: Min-Jie
authors.initials: MJ
authors.lastname: Wei
authors.forename: Xian-Zheng
authors.initials: XZ
authors.lastname: Sha
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000009:adverse effects / Q000737:chemistry*; D048271:Chitosan / Q000009:adverse effects / Q000737:chemistry*; D005465:Fluorocarbon Polymers / Q000737:chemistry*; D005549:Foreign-Body Reaction / Q000209:etiology / Q000473:pathology; D011138:Polytetrafluoroethylene / Q000009:adverse effects / Q000737:chemistry*; D016062:Porosity; D051381:Rats; D017207:Rats, Sprague-Dawley; D054457:Tissue Scaffolds / Q000009:adverse effects / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D005465:Fluorocarbon Polymers; C040402:perfluorosulfonic acid; D011138:Polytetrafluoroethylene; D048271:Chitosan
keywords: biocompatibility; chitosan; foreign body response; implantable sensor; porous composite