title: Neuroprotective effects of resveratrol in an MPTP mouse model of Parkinson's-like disease: possible role of SOCS-1 in reducing pro-inflammatory responses.
abstract: In the present study we used a 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced Parkinson's disease (PD) mouse model to analyze resveratrol neuroprotective effects. The MPTP-induced PD model is characterized by chronic inflammation, oxidative stress and loss of the dopaminergic (DA) neurons in the substantia nigra pars compacta (SNpc). We observed that resveratrol treatment significantly reduced glial activation, decreasing the levels of IL-1β, IL-6 and TNF-α, as well as their respective receptors in the SNpc of MPTP-treated mice, as demonstrated by Western blotting, RT-PCR and quantitative PCR analysis. This reduction is related to possible neuroprotection as we also observed that resveratrol administration limited the decline of tyrosine hydroxylase-immunoreactivity induced in the striatum and SNpc by MPTP injection. Consistent with these data, resveratrol treatment up-regulated the expression of the suppressor of cytokine signaling-1 (SOCS-1), supporting the hypothesis that resveratrol protects DA neurons of the SNpc against MPTP-induced cell loss by regulating inflammatory reactions, possibly through SOCS-1 induction.
authors.affiliation: 1Department of Biological and Environmental Sciences and Technologies, Section of Human Anatomy, University of Salento, Lecce, Italy.
authors.forename: Dario D
authors.initials: DD
authors.lastname: Lofrumento
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Nicolardi
authors.forename: Antonia
authors.initials: A
authors.lastname: Cianciulli
authors.forename: Francesco
authors.initials: F
authors.lastname: De Nuccio
authors.forename: Velia
authors.initials: V
authors.lastname: La Pesa
authors.forename: Vito
authors.initials: V
authors.lastname: Carofiglio
authors.forename: Teresa
authors.initials: T
authors.lastname: Dragone
authors.forename: Rosa
authors.initials: R
authors.lastname: Calvello
authors.forename: Maria A
authors.initials: MA
authors.lastname: Panaro
mesh_terms: D000818:Animals; D016207:Cytokines / Q000096:biosynthesis; D059290:Dopaminergic Neurons / Q000187:drug effects / Q000473:pathology; D007150:Immunohistochemistry; D007249:Inflammation / Q000188:drug therapy* / Q000473:pathology; D020267:MPTP Poisoning / Q000188:drug therapy* / Q000276:immunology / Q000473:pathology; D008262:Macrophage Activation / Q000187:drug effects; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D017072:Neostriatum / Q000378:metabolism; D009457:Neuroglia / Q000187:drug effects; D018696:Neuroprotective Agents / Q000627:therapeutic use*; D060888:Real-Time Polymerase Chain Reaction; D000077185:Resveratrol; D013267:Stilbenes / Q000627:therapeutic use*; D013378:Substantia Nigra / Q000378:metabolism; D000071222:Suppressor of Cytokine Signaling 1 Protein; D050826:Suppressor of Cytokine Signaling Proteins / Q000096:biosynthesis / Q000502:physiology*; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D016207:Cytokines; D018696:Neuroprotective Agents; C491590:Socs1 protein, mouse; D013267:Stilbenes; D000071222:Suppressor of Cytokine Signaling 1 Protein; D050826:Suppressor of Cytokine Signaling Proteins; D014446:Tyrosine 3-Monooxygenase; D000077185:Resveratrol
keywords: Parkinson’s disease; SOCS-1; cytokine; dopaminergic neuron; resveratrol