title: [Association study for gene polymorphism of folic acid/homocysteine metabolic pathway and nonsyndromic cleft lip with or without cleft palate in Chinese populations].
abstract: OBJECTIVE
  To explore the association between 18 candidate genes encoding enzymes on the folate/homocysteine metabolism pathway and non-syndromic cleft lip with or without cleft palate (NSCL/P) in Chinese populations.
  
  
  METHODS
  A total of 806 NSCL/P trios were drawn by an international consortium, which conducted a genome-wide association study using a case-parent trio design to investigate genes affecting risks to NSCL/P. The transmission disequilibrium test (TDT) was used for deviation from Mendelian expectations for 257 SNPs in 18 folate/homocysteine metabolism-related genes. The interactions between markers in these gene and environmental risk factors were also tested using conditional Logistic regressions.
  
  
  RESULTS
  Although four SNPs (rs6428977, rs12060264, rs7730643 and rs4920037) showed nominal significant association with NSCL/P in the TDT on 806 NSCL/P trios (P<0.05), no significant evidence of linkage and association remained in all the SNPs after Bonferroni correction. Similar tests for interactions between genes and maternal smoking, environmental tobacco smoke, alcohol consumption and multi-vitamin supplementation during pregnancy did not attain statistical significance after correction for multiple comparisons.
  
  
  CONCLUSIONS
  Folate/homocysteine metabolism-related genes could not influence the risk of NSCL/P.
authors.affiliation: Department of Epidemiology and Biostatistics, Peking University School of Public Health, Beijing, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Wang
authors.forename: Hong
authors.initials: H
authors.lastname: Wang
authors.forename: Yah-huei
authors.initials: YH
authors.lastname: Wu-chou
authors.forename: Xiao-qian
authors.initials: XQ
authors.lastname: Ye
authors.forename: Shang-zhi
authors.initials: SZ
authors.lastname: Huang
authors.forename: Bing
authors.initials: B
authors.lastname: Shi
authors.forename: Kung-yee
authors.initials: KY
authors.lastname: Liang
authors.forename: Wei-hua
authors.initials: WH
authors.lastname: Cao
authors.forename: Tao
authors.initials: T
authors.lastname: Wu
authors.forename: Terei H
authors.initials: TH
authors.lastname: Beaty
mesh_terms: D044466:Asian People; D002971:Cleft Lip / Q000235:genetics*; D002972:Cleft Palate / Q000235:genetics*; D005492:Folic Acid / Q000096:biosynthesis*; D008040:Genetic Linkage; D055106:Genome-Wide Association Study; D006710:Homocysteine / Q000096:biosynthesis*; D006801:Humans; D016015:Logistic Models; D053858:Metabolic Networks and Pathways / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D012307:Risk Factors
chemical_list: D006710:Homocysteine; D005492:Folic Acid