title: RP-HPLC method using one marker for quantification of four podophyllum lignans in medicinal plants.
abstract: A high-performance liquid chromatographic method using a single standard has been established for the quantitative analysis of four podophyllum lignans in Dysosma versipellis (Hance) M. Cheng and Podophyllum emodi Wall. Var. chinesis Sprague. The method involved the quantitative analysis of multiple components by a single marker. The chromatographic method was validated for linearity and range, limit of detection and qualification, precision, stability, reproducibility and robustness. Relative correcting factors were calculated and examined by five concentrations of four podophyllum lignans, two high-performance liquid chromatographic systems and three chromatographic columns. The method was applied to analyze 10 batches of samples. The quantitative results were compared with the results by an external standard method through intra-class coefficient, which indicated that the established method was reliable for the determination of the four podophyllum lignans in the two medicinal plants.
authors.affiliation: School of Pharmacy, Lanzhou University, Lanzhou 730000, Gansu Province, P. R. China Jiangsu Provincial Key Laboratory for Interventional Medical Devices, Huaiyin Institute of Technology, Huaian 223003, Jiangsu Province, P. R. China.
authors.forename: Ningwei
authors.initials: N
authors.lastname: Lu
authors.affiliation: School of Pharmacy, Lanzhou University, Lanzhou 730000, Gansu Province, P. R. China.
authors.forename: Qiong
authors.initials: Q
authors.lastname: An
authors.affiliation: School of Pharmacy, Lanzhou University, Lanzhou 730000, Gansu Province, P. R. China.
authors.forename: Ning
authors.initials: N
authors.lastname: Li
authors.affiliation: School of Pharmacy, Lanzhou University, Lanzhou 730000, Gansu Province, P. R. China dongym@lzu.edu.cn.
authors.forename: Yuming
authors.initials: Y
authors.lastname: Dong
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D056148:Chromatography, Reverse-Phase / Q000379:methods*; D017705:Lignans / Q000032:analysis*; D010946:Plants, Medicinal / Q000737:chemistry*; D011035:Podophyllum / Q000737:chemistry*; D015203:Reproducibility of Results
chemical_list: D017705:Lignans