title: Anti-cancer activity of quercetin in neuroblastoma: an in vitro approach.
abstract: Neuroblastoma is a neuroendocrine tumour derived from neural crest cells and it remains a major therapeutic challenge in pediatric oncology. As response rates to chemotherapy are low, surgery remains the only effective treatment but since many tumors have metastasized at the time of diagnosis, curative surgery is rarely achieved. Consequently, a substantial need for new therapeutic options emerges. Quercetin a flavonoid, has been reported to lower the risk of several cancers. This study was designed to investigate its effects on apoptosis induction in the N2a, a mouse neuroblastoma cell line. The cell viability was determined by dimethyl thiazolyl tetrazolium bromide assay and diamidino-2-phenylindole staining was performed to confirm the apoptosis. The gene expression of bcl-w, p53, p27 and protein expression of caspases (3 and 9), bax, cytochrome-c were studied. This in vitro outcome suggests that quercetin can be used as a potent anti-cancer drug in future.
authors.affiliation: Department of Endocrinology, Dr. ALM. Post Graduate Institute of Basic Medical Sciences, University of Madras, Taramani Campus, Chennai, 600113, India.
authors.forename: E
authors.initials: E
authors.lastname: Sugantha Priya
authors.forename: K
authors.initials: K
authors.lastname: Selvakumar
authors.forename: S
authors.initials: S
authors.lastname: Bavithra
authors.forename: P
authors.initials: P
authors.lastname: Elumalai
authors.forename: R
authors.initials: R
authors.lastname: Arunkumar
authors.forename: P
authors.initials: P
authors.lastname: Raja Singh
authors.forename: A
authors.initials: A
authors.lastname: Brindha Mercy
authors.forename: J
authors.initials: J
authors.lastname: Arunakaran
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects* / Q000502:physiology; D051017:Apoptosis Regulatory Proteins; D015153:Blotting, Western; D053148:Caspase 3 / Q000378:metabolism; D053453:Caspase 9 / Q000378:metabolism; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D045304:Cytochromes c / Q000378:metabolism; D015870:Gene Expression / Q000187:drug effects; D007211:Indoles; D051379:Mice; D009447:Neuroblastoma / Q000188:drug therapy* / Q000503:physiopathology; D016133:Polymerase Chain Reaction; D011506:Proteins / Q000378:metabolism; D011794:Quercetin / Q000494:pharmacology*; D012333:RNA, Messenger / Q000378:metabolism; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism; D051028:bcl-2-Associated X Protein / Q000378:metabolism
chemical_list: D000970:Antineoplastic Agents; D051017:Apoptosis Regulatory Proteins; C494827:Bax protein, mouse; C101155:Bcl2l2 protein, mouse; D007211:Indoles; D011506:Proteins; D012333:RNA, Messenger; D016159:Tumor Suppressor Protein p53; D051028:bcl-2-Associated X Protein; C007293:DAPI; D045304:Cytochromes c; D011794:Quercetin; C505409:Casp3 protein, mouse; C506434:Casp9 protein, mouse; D053148:Caspase 3; D053453:Caspase 9