title: An overview of proteomics approaches applied to biopharmaceuticals and cyclotides research.
abstract: The evolution in proteomics approaches is notable, including quantitative proteomics and strategies for elucidation of post-translational modifications. Faster and more accurate mass spectrometers as well as cleverer bioinformatics tolls are making the difference in such advancement. Among the wide range of research in plant proteomics, biopharmaceutical production using plants as "biofactories" and the screening of new activities of new molecules, in this case, peptides, are quite important regarding translational proteomics. The present review is focused on "recombinant proteins and bioactive peptides", with biopharmaceuticals and cyclotides chosen as examples. Their application and challenges are focused on a "translational proteomics" point of view, in order to exemplify some new areas of research based on proteomics strategies. This article is part of a Special Issue entitled: Translational Plant Proteomics.
authors.affiliation: Center of Biotechnology, Universidade Federal do Rio Grande do Sul., Av. Bento Gonçalves 9500, Prédio 43431, Sala 214, Porto Alegre, RS 91501-970, Brazil. Electronic address: diogord@terra.com.br.
authors.forename: Diogo Ribeiro
authors.initials: DR
authors.lastname: Demartini
authors.forename: Giancarlo
authors.initials: G
authors.lastname: Pasquali
authors.forename: Célia Regina
authors.initials: CR
authors.lastname: Carlini
mesh_terms: D000911:Antibodies, Monoclonal / Q000096:biosynthesis; D000941:Antigens / Q000096:biosynthesis; D047169:Cyclotides / Q000096:biosynthesis*; D006801:Humans; D007328:Insulin / Q000096:biosynthesis; D013058:Mass Spectrometry; D010944:Plants / Q000378:metabolism*; D011499:Protein Processing, Post-Translational; D040901:Proteomics / Q000379:methods*; D011994:Recombinant Proteins / Q000096:biosynthesis*
chemical_list: D000911:Antibodies, Monoclonal; D000941:Antigens; D047169:Cyclotides; D007328:Insulin; D011994:Recombinant Proteins
keywords: Biopharmaceuticals; Cyclotide; High resolution mass spectrometry; Molecular farming; Plant translational proteomics; Post-translational modification