title: Fatty alcohol production in engineered E. coli expressing Marinobacter fatty acyl-CoA reductases.
abstract: Although successful production of fatty alcohols in metabolically engineered Escherichia coli with heterologous expression of fatty acyl-CoA reductase has been reported, low biosynthetic efficiency is still a hurdle to be overcome. In this study, we examined the characteristics of two fatty acyl-CoA reductases encoded by Maqu_2220 and Maqu_2507 genes from Marinobacter aquaeolei VT8 on fatty alcohol production in E. coli. Fatty alcohols with diversified carbon chain length were obtained by co-expressing Maqu_2220 with different carbon chain length-specific acyl-ACP thioesterases. Both fatty acyl-CoA reductases displayed broad substrate specificities for C12-C18 fatty acyl chains in vivo. The optimized mutant strain of E. coli carrying the modified tesA gene and fadD gene from E. coli and Maqu_2220 gene from Marinobacter aquaeolei VT8 produced fatty alcohols at a remarkable level of 1.725 g/L under the fermentation condition.
authors.affiliation: Key Laboratory of Biofuels, Shandong Provincial Key Laboratory of Energy Genetics, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, No. 189 Songling Road, Qingdao, 266101, China.
authors.forename: Aiqiu
authors.initials: A
authors.lastname: Liu
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Tan
authors.forename: Lun
authors.initials: L
authors.lastname: Yao
authors.forename: Xuefeng
authors.initials: X
authors.lastname: Lu
mesh_terms: D000445:Aldehyde Oxidoreductases / Q000235:genetics*; D001483:Base Sequence; D017931:DNA Primers; D004926:Escherichia coli / Q000235:genetics*; D005233:Fatty Alcohols / Q000378:metabolism*; D005285:Fermentation; D053520:Marinobacter / Q000235:genetics*; D015202:Protein Engineering
chemical_list: D017931:DNA Primers; D005233:Fatty Alcohols; D000445:Aldehyde Oxidoreductases; C021351:hexadecanal dehydrogenase (acylating)