title: A reusable sensor for the label-free detection of specific oligonucleotides by surface plasmon fluorescence spectroscopy.
abstract: The development of a reusable molecular beacon (MB)-based sensor for the label-free detection of specific oligonucleotides using surface plasmon fluorescence spectroscopy (SPFS) as the readout method is described. The MBs are chemisorbed at planar gold surfaces serving as fluorescence quenching units. Target oligonucleotides of 24 bases can be detected within a few minutes at high single-mismatch discrimination rates.
authors.affiliation: Nöll Junior Research Group for Nanotechnology, Siegen University, Faculty IV, Department of Chemistry-Biology, Organic Chemistry, Adolf-Reichwein-Str. 2, 57068 Siegen, Germany. noell@chemie.uni-siegen.de.
authors.forename: Gilbert
authors.initials: G
authors.lastname: Nöll
authors.forename: Qiang
authors.initials: Q
authors.lastname: Su
authors.forename: Björn
authors.initials: B
authors.lastname: Heidel
authors.forename: Yaming
authors.initials: Y
authors.lastname: Yu
mesh_terms: D020137:Base Pair Mismatch; D005456:Fluorescent Dyes / Q000737:chemistry; D006046:Gold / Q000737:chemistry; D009693:Nucleic Acid Hybridization; D009841:Oligonucleotides / Q000032:analysis* / Q000737:chemistry; D020349:Surface Plasmon Resonance; D013499:Surface Properties
chemical_list: D005456:Fluorescent Dyes; D009841:Oligonucleotides; D006046:Gold
keywords: DNA; molecular beacon (MB); oligonucleotides; single-nucleotide polymorphism; surface plasmon fluorescence spectroscopy (SPFS)