title: Gene expression responses in Suaeda salsa after cadmium exposure.
abstract: Coastal line is now polluted by many kinds of sewage including heavy metals discharged by intensive human activities. Cadmium is a nonessential heavy metal for organisms and can cause many kinds of adverse effect on the organisms. Suaeda salsa, a pioneer halophyte in intertidal zone of the Bohai coast, was proved to have cadmium-tolerant capacity. Given that, S. salsa was suggested as a potential coastal bio-indicator plant for cadmium contamination in the intertidal zone. Therefore, it is essential to investigate the responsive mechanism of S. salsa to cadmium since few studies focus on this subject till now. In the present study, six genes were selected to investigate the variation profiles of mRNA expression by fluorescent real-time quantitative PCR, including those involved in myo-inositol synthesis, redox reaction, salt-tolerant reaction. Results showed that cadmium exposure significantly modulate the mRNA expressions of MIPS, Nhx1, CAT2, GST, Prx Q genes. It suggested that cadmium exposure exerted an oxidative stress on S. salsa, disturbed Na(+) homeostasis across membranes and interfered with the metabolism of inositol. In addition, CAT2 gene could be used as a gene marker in S. salsa to indicate cadmium pollution.
authors.affiliation: Key Laboratory of Coastal Zone Environmental Processes, Yantai Institute of Coastal Zone Research(YIC), Chinese Academy of Sciences(CAS); Shandong Provincial Key Laboratory of Coastal Zone Environmental Processes, YICCAS, Yantai Shandong, 264003 P. R. China.
authors.forename: Ming
authors.initials: M
authors.lastname: Cong
authors.forename: Jiasen
authors.initials: J
authors.lastname: Lv
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Liu
authors.forename: Jianmin
authors.initials: J
authors.lastname: Zhao
authors.forename: Huifeng
authors.initials: H
authors.lastname: Wu
keywords: Cadmium; Gene expression; Suaeda salsa; qRT-PCR