title: Role of sortase-dependent pili of Bifidobacterium bifidum PRL2010 in modulating bacterium-host interactions.
abstract: Bifidobacteria represent one of the dominant groups of microorganisms colonizing the human infant intestine. Commensal bacteria that interact with a eukaryotic host are believed to express adhesive molecules on their cell surface that bind to specific host cell receptors or soluble macromolecules. Whole-genome transcription profiling of Bifidobacterium bifidum PRL2010, a strain isolated from infant stool, revealed a small number of commonly expressed extracellular proteins, among which were genes that specify sortase-dependent pili. Expression of the coding sequences of these B. bifidum PRL2010 appendages in nonpiliated Lactococcus lactis enhanced adherence to human enterocytes through extracellular matrix protein and bacterial aggregation. Furthermore, such piliated L. lactis cells evoked a higher TNF-α response during murine colonization compared with their nonpiliated parent, suggesting that bifidobacterial sortase-dependent pili not only contribute to adherence but also display immunomodulatory activity.
authors.affiliation: Department of Microbiology, Alimentary Pharmabiotic Centre, Bioscience Institute, National University of Ireland, Cork, Ireland.
authors.forename: Francesca
authors.initials: F
authors.lastname: Turroni
authors.forename: Fausta
authors.initials: F
authors.lastname: Serafini
authors.forename: Elena
authors.initials: E
authors.lastname: Foroni
authors.forename: Sabrina
authors.initials: S
authors.lastname: Duranti
authors.forename: Mary
authors.initials: M
authors.lastname: O'Connell Motherway
authors.forename: Valentina
authors.initials: V
authors.lastname: Taverniti
authors.forename: Marta
authors.initials: M
authors.lastname: Mangifesta
authors.forename: Christian
authors.initials: C
authors.lastname: Milani
authors.forename: Alice
authors.initials: A
authors.lastname: Viappiani
authors.forename: Tommaso
authors.initials: T
authors.lastname: Roversi
authors.forename: Borja
authors.initials: B
authors.lastname: Sánchez
authors.forename: Andrea
authors.initials: A
authors.lastname: Santoni
authors.forename: Laura
authors.initials: L
authors.lastname: Gioiosa
authors.forename: Alberto
authors.initials: A
authors.lastname: Ferrarini
authors.forename: Massimo
authors.initials: M
authors.lastname: Delledonne
authors.forename: Abelardo
authors.initials: A
authors.lastname: Margolles
authors.forename: Laura
authors.initials: L
authors.lastname: Piazza
authors.forename: Paola
authors.initials: P
authors.lastname: Palanza
authors.forename: Angelo
authors.initials: A
authors.lastname: Bolchi
authors.forename: Simone
authors.initials: S
authors.lastname: Guglielmetti
authors.forename: Douwe
authors.initials: D
authors.lastname: van Sinderen
authors.forename: Marco
authors.initials: M
authors.lastname: Ventura
mesh_terms: D019881:Aminoacyltransferases / Q000235:genetics / Q000378:metabolism; D000818:Animals; D001422:Bacterial Adhesion / Q000235:genetics / Q000276:immunology; D001426:Bacterial Proteins / Q000235:genetics / Q000502:physiology; D001644:Bifidobacterium / Q000235:genetics / Q000276:immunology / Q000502:physiology*; D002460:Cell Line; D003546:Cysteine Endopeptidases / Q000235:genetics / Q000378:metabolism; D016207:Cytokines / Q000096:biosynthesis; D005260:Female; D010861:Fimbriae, Bacterial / Q000235:genetics / Q000276:immunology / Q000502:physiology*; D005798:Genes, Bacterial; D006801:Humans; D007223:Infant; D007413:Intestinal Mucosa / Q000276:immunology / Q000382:microbiology; D013294:Lactococcus lactis / Q000235:genetics / Q000502:physiology; D051379:Mice; D008807:Mice, Inbred BALB C; D019936:Probiotics; D059467:Transcriptome / Q000276:immunology
chemical_list: D001426:Bacterial Proteins; D016207:Cytokines; D019881:Aminoacyltransferases; D003546:Cysteine Endopeptidases
keywords: genomics; host–microbe interaction; probiotics; transcriptomics