title: Effect of Kushen (Radix Sophorae flavescentis) extract on laryngeal neoplasm Hep2 cells.
abstract: OBJECTIVE
  To evaluate the effect of fermented extract of Kushen (Radix Sophorae Flavescentis) or non-fermented ESF on laryngeal neoplasms Hep2 cells.
  
  
  METHODS
  Use 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay to explore the effect of fermented ESF and non-fermented ESF on Hep2 cells, and detect the mRNA and protein expression level of Bcl-2, Bax and Caspase-3 with reverse transcription polymerase chain reaction (RT-PCR) and Western blot.
  
  
  RESULTS
  Both fermented ESF and non-fermented ESF could inhibit laryngeal neoplasm's Hep2 cells, but and the cells did not response to the dilution 1: 320 of fermented ESF, nor to the 1:1280 dilution of non-fermented ESF. As time progressed, the dilution 1:80 of fermented ESF and 1:320 dilution of non-fermented ESF could significantly reduce Bcl-2 mRNA and protein expression and down-regulate Caspase-3 mRNA and protein expression. Bax mRNA and protein were not expressed in Hep2 cells.
  
  
  CONCLUSIONS
  Both fermented ESF and non-fermented ESF could inhibit the proliferation of Hep2 cells, and the effect of non-fermented ESF was significantly better than that of the fermented.
authors.affiliation: School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China. wangyzi@sina.com
authors.forename: Yingzi
authors.initials: Y
authors.lastname: Wang
authors.forename: Chunchao
authors.initials: C
authors.lastname: Han
authors.forename: Xinsheng
authors.initials: X
authors.lastname: Fang
authors.forename: Xinyuan
authors.initials: X
authors.lastname: Shi
authors.forename: Ailing
authors.initials: A
authors.lastname: Feng
authors.forename: Kuibang
authors.initials: K
authors.lastname: He
authors.forename: Shenghai
authors.initials: S
authors.lastname: Zhang
authors.forename: Xiuyu
authors.initials: X
authors.lastname: Sun
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D053148:Caspase 3 / Q000235:genetics / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000494:pharmacology*; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D007822:Laryngeal Neoplasms / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism / Q000503:physiopathology; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics / Q000378:metabolism; D051028:bcl-2-Associated X Protein / Q000235:genetics / Q000378:metabolism
chemical_list: D004365:Drugs, Chinese Herbal; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein; D053148:Caspase 3; C494416:kushen