title: BSA and fibrinogen adsorption on chitosan/κ-carrageenan polyelectrolyte complexes.
abstract: PECs of chitosan/κ-carrageenan are prepared in three different volumetric rations. The complex formation is characterized in order to evaluate the blending formation. Blood compatibility is evaluated by protein adsorption (BSA and fibrinogen) and PEC toxicities are determined with fibroblast cell viability and proliferation. The swelling degree of PECs decreases when the amount of chitosan increases. Due to the linked film formation, PECs decrease BSA adsorption and increase fibrinogen adsorption when compared to the pristine chitosan and κ-carrageenan films. Although pristine chitosan and κ-carrageenan films produced similar cell expansion and viability, the PEC 50:50 vol% chitosan/κ-carrageenan PEC may be acceptable as a new scaffold for cell therapies, due to their effect on cell survival.
authors.affiliation: Departamento de Engenharia Química, Universidade Federal do Ceará, Centro de Tecnologia, Av. Mister Hull, s/n, Campus do Pici, Bloco 709 Pici, CEP 60455-760 Fortaleza, CE, Brazil.
authors.forename: Thiane N
authors.initials: TN
authors.lastname: Carneiro
authors.forename: Denise S
authors.initials: DS
authors.lastname: Novaes
authors.forename: Rodrigo B
authors.initials: RB
authors.lastname: Rabelo
authors.forename: Betul
authors.initials: B
authors.lastname: Celebi
authors.forename: Pascale
authors.initials: P
authors.lastname: Chevallier
authors.forename: Diego
authors.initials: D
authors.lastname: Mantovani
authors.forename: Marisa M
authors.initials: MM
authors.lastname: Beppu
authors.forename: Rodrigo S
authors.initials: RS
authors.lastname: Vieira
mesh_terms: D016475:3T3 Cells; D000327:Adsorption; D000818:Animals; D001672:Biocompatible Materials / Q000138:chemical synthesis / Q000378:metabolism*; D002351:Carrageenan / Q000378:metabolism*; D002460:Cell Line; D049109:Cell Proliferation; D002470:Cell Survival; D064987:Cell- and Tissue-Based Therapy / Q000379:methods; D048271:Chitosan / Q000378:metabolism*; D004573:Electrolytes / Q000138:chemical synthesis; D005340:Fibrinogen / Q000378:metabolism*; D005347:Fibroblasts / Q000378:metabolism; D051379:Mice; D018625:Microscopy, Atomic Force; D012710:Serum Albumin, Bovine / Q000378:metabolism*; D013499:Surface Properties; D054457:Tissue Scaffolds
chemical_list: D001672:Biocompatible Materials; D004573:Electrolytes; D012710:Serum Albumin, Bovine; D002351:Carrageenan; D005340:Fibrinogen; D048271:Chitosan
keywords: cell proliferation; chitosan; polyelectrolyte complex; protein adsorption; κ-carrageenan