title: Synthesis of mesoporous multiwall ZnO nanotubes by replicating silk and application for enzymatic biosensor.
abstract: A facial biotemplate-directed synthetic route for fabricating mesoporous multiwall ZnO nanotubes (ZnO-MMNTs) was proposed. Owing to the mesoporous multiwall based matrix, one dimensional (1D) tubes based channels and high isoelectric point (IEP), the prepared ZnO-MMNTs are wonderful platform to immobilize glucose oxidase (GOx) for glucose biosensing. Scale-adaptive cells are constructed to hold enzymes molecules, maintain enzymatic activity and keep stability. The prepared enzymatic electrode (chitosan/GOx/ZnO/Au) exhibits high sensitivity (47.2 μA mM(-1)cm(-2)), very fast response (<2s), quite low KM(app) (1.09 mM), excellent selectivity and stability. Therefore, ZnO-MMNTs are promising material for enzyme assembly and other biological applications.
authors.affiliation: Department of Materials Science and Engineering, State Key Laboratory of Silicon Materials, Zhejiang University, Hangzhou 310027, China.
authors.forename: Minggang
authors.initials: M
authors.lastname: Zhao
authors.forename: Zhenglong
authors.initials: Z
authors.lastname: Li
authors.forename: Ziqi
authors.initials: Z
authors.lastname: Han
authors.forename: Ke
authors.initials: K
authors.lastname: Wang
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhou
authors.forename: Jingyun
authors.initials: J
authors.lastname: Huang
authors.forename: Zhizhen
authors.initials: Z
authors.lastname: Ye
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D048271:Chitosan / Q000737:chemistry; D055664:Electrochemical Techniques / Q000379:methods; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism*; D005947:Glucose / Q000032:analysis* / Q000378:metabolism; D005949:Glucose Oxidase / Q000737:chemistry / Q000378:metabolism*; D006046:Gold / Q000737:chemistry; D057230:Limit of Detection; D043942:Nanotubes / Q000737:chemistry* / Q000648:ultrastructure; D016062:Porosity; D015034:Zinc Oxide / Q000737:chemistry*
chemical_list: D004800:Enzymes, Immobilized; D006046:Gold; D048271:Chitosan; D005949:Glucose Oxidase; D005947:Glucose; D015034:Zinc Oxide
keywords: Electrochemical biosensor; Glucose oxidase; Mesoporous multiwall nanotubes; ZnO