title: Highly selective and sensitive detection of neurotransmitters using receptor-modified single-walled carbon nanotube sensors.
abstract: We present receptor-modified carbon nanotube sensors for the highly selective and sensitive detection of acetylcholine (ACh), one kind of neurotransmitter. Here, we successfully expressed the M1 muscarinic acetylcholine receptor (M1 mAChR), a family of G protein-coupled receptors (GPCRs), in E. coli and coated single-walled carbon nanotube (swCNT)-field effect transistors (FETs) with lipid membrane including the receptor, enabling highly selective and sensitive ACh detection. Using this sensor, we could detect ACh at 100 pM concentration. Moreover, we showed that this sensor could selectively detect ACh among other neurotransmitters. This is the first demonstration of the real-time detection of ACh using specific binding between ACh and M1 mAChR, and it may lead to breakthroughs for various applications such as disease diagnosis and drug screening.
authors.affiliation: Department of Physics and Astronomy, Seoul National University, Seoul 151-747, Korea.
authors.forename: Byeongju
authors.initials: B
authors.lastname: Kim
authors.forename: Hyun Seok
authors.initials: HS
authors.lastname: Song
authors.forename: Hye Jun
authors.initials: HJ
authors.lastname: Jin
authors.forename: Eun Jin
authors.initials: EJ
authors.lastname: Park
authors.forename: Sang Hun
authors.initials: SH
authors.lastname: Lee
authors.forename: Byung Yang
authors.initials: BY
authors.lastname: Lee
authors.forename: Tai Hyun
authors.initials: TH
authors.lastname: Park
authors.forename: Seunghun
authors.initials: S
authors.lastname: Hong
mesh_terms: D000109:Acetylcholine / Q000032:analysis / Q000378:metabolism; D015374:Biosensing Techniques / Q000379:methods*; D015153:Blotting, Western; D055664:Electrochemical Techniques; D055767:Immobilized Proteins / Q000737:chemistry / Q000378:metabolism; D008958:Models, Molecular; D037742:Nanotubes, Carbon / Q000737:chemistry*; D018377:Neurotransmitter Agents / Q000032:analysis* / Q000378:metabolism; D011976:Receptors, Muscarinic / Q000737:chemistry* / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism
chemical_list: D055767:Immobilized Proteins; D037742:Nanotubes, Carbon; D018377:Neurotransmitter Agents; D011976:Receptors, Muscarinic; D011994:Recombinant Proteins; D000109:Acetylcholine