title: Application of pH-sensitive fusogenic polymer-modified liposomes for development of mucosal vaccines.
abstract: To evaluate the usefulness of pH-sensitive fusogenic polymer (succinylated poly(glycidol) (SucPG) and 3-methylglutarylated poly(glycidol) (MGluPG))-modified liposomes as mucosal vaccine in the induction of a protective immune responses was evaluated. Mice were nasally immunized with OVA-containing SucPG-modified liposomes. After immunization, significant Ag-specific Abs were detected in the serum and intestine. When sera were analyzed for isotype distribution, antigen-specific IgG1 Ab responses were noted in mice immunized with OVA-containing polymer-unmodified liposomes, whereas immunization with OVA-containing SucPG-modified liposomes resulted in the induction of OVA-specific IgG1, IgG2a and IgG3 Ab responses. In spleen lymphocytes from mice immunized with OVA-containing SucPG-modified liposomes, both IFN-γ and IL-4 mRNA were detected. The same result was obtained also in the mouse immunized with OVA-containing MGluPG-modified liposomes. Furthermore, we examined the induction of immune responses in chickens following intraocular immunization with Salmonella Enteritidis Ag-containing MGluPG-modified liposomes, and the protective effect against the challenge with S. Enteritidis. Immunization with S. Enteritidis Ag-containing MGluPG-modified liposomes induced significant Ab responses against S. Enteritidis in the serum and intestine. Less fecal excretion of bacteria was observed in chickens immunized with S. Enteritidis Ag-containing MGluPG-modified liposomes after challenge. The numbers of bacteria in the caecum were also lower in immunized chickens than in unimmunized controls.
authors.affiliation: Division of Veterinary Science, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Izumisano, Osaka 598-8531, Japan. Electronic address: swatarai@vet.osakafu-u.ac.jp.
authors.forename: Shinobu
authors.initials: S
authors.lastname: Watarai
authors.affiliation: Division of Veterinary Science, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Izumisano, Osaka 598-8531, Japan.
authors.forename: Tana
authors.initials: T
authors.lastname: Iwase
authors.affiliation: Division of Veterinary Science, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Izumisano, Osaka 598-8531, Japan.
authors.forename: Tomoko
authors.initials: T
authors.lastname: Tajima
authors.affiliation: Department of Applied Chemistry, Graduate School of Engineering, Osaka Prefecture University, Sakai, Osaka 599-8531, Japan.
authors.forename: Eiji
authors.initials: E
authors.lastname: Yuba
authors.affiliation: Department of Applied Chemistry, Graduate School of Engineering, Osaka Prefecture University, Sakai, Osaka 599-8531, Japan.
authors.forename: Kenji
authors.initials: K
authors.lastname: Kono
authors.affiliation: Research Institute, Nippon Biologicals, Inc., Koriyama, Fukushima 963-0196, Japan.
authors.forename: Yukio
authors.initials: Y
authors.lastname: Sekiya
mesh_terms: D000818:Animals; D002645:Chickens; D005243:Feces / Q000382:microbiology; D005260:Female; D007114:Immunization / Q000662:veterinary; D007074:Immunoglobulin G / Q000097:blood; D007371:Interferon-gamma / Q000097:blood / Q000235:genetics; D015847:Interleukin-4 / Q000097:blood / Q000235:genetics; D008081:Liposomes / Q000494:pharmacology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009297:Nasal Mucosa / Q000276:immunology / Q000382:microbiology; D011201:Poultry Diseases / Q000276:immunology / Q000382:microbiology*; D011409:Propylene Glycols / Q000494:pharmacology; D012333:RNA, Messenger / Q000737:chemistry / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D012481:Salmonella Infections, Animal / Q000097:blood / Q000276:immunology / Q000382:microbiology* / Q000517:prevention & control; D022562:Salmonella Vaccines / Q000494:pharmacology*; D012477:Salmonella enteritidis / Q000276:immunology*; D013047:Specific Pathogen-Free Organisms
chemical_list: D007074:Immunoglobulin G; D008081:Liposomes; D011409:Propylene Glycols; D012333:RNA, Messenger; D022562:Salmonella Vaccines; C502907:polyglycidol; D015847:Interleukin-4; D007371:Interferon-gamma
keywords: Fusogenic liposomes; Mucosal vaccines; Protective immunity; Salmonella Enteritidis; Th1