title: In vitro evaluation of the effect of mouth rinse with trimetaphosphate on enamel demineralization.
abstract: OBJECTIVE
  The aim of this study was to investigate the effectiveness of sodium trimetaphosphate (TMP) addition to mouth rinses to inhibit enamel demineralization.
  
  
  METHODS
  Bovine enamel blocks (n = 88) were selected by surface hardness and divided into eight treatment groups (n = 11 per group): placebo, 100 or 225 μg F/ml; the rinses with 100 μg F/ml had differing TMP concentrations (range 0-0.6%). The blocks were subjected to pH cycling for 5 days and treated twice a day with mouth rinses. After that, surface and cross-sectional hardness as well as fluoride in enamel were measured.
  
  
  RESULTS
  The groups containing both 100 μg F/ml and 0.4% TMP inhibited demineralization most effectively (p < 0.001). This formulation yielded lower values of lesion areas than the formulations containing 100 or 225 μg F/ml but no TMP. The addition of 0.4% TMP increased the fluoride in enamel.
  
  
  CONCLUSIONS
  It is possible to improve the effectiveness of a mouth rinse with 100 µg F/ml by addition of TMP, this being superior in inhibiting enamel demineralization compared with mouth rinses containing 225 µg F/ml.
authors.affiliation: Faculdade de Odontologia, Universidade Estadual Paulista (UNESP), Araçatuba, Brazil.
authors.forename: C O
authors.initials: CO
authors.lastname: Favretto
authors.forename: M
authors.initials: M
authors.lastname: Danelon
authors.forename: F C N
authors.initials: FC
authors.lastname: Castilho
authors.forename: A E M
authors.initials: AE
authors.lastname: Vieira
authors.forename: A C B
authors.initials: AC
authors.lastname: Delbem
mesh_terms: D000818:Animals; D002327:Cariostatic Agents / Q000008:administration & dosage / Q000627:therapeutic use*; D002417:Cattle; D002626:Chemistry, Pharmaceutical; D003743:Dental Enamel / Q000737:chemistry / Q000187:drug effects*; D005459:Fluorides / Q000032:analysis; D006244:Hardness; D006863:Hydrogen-Ion Concentration; D008422:Materials Testing; D009067:Mouthwashes / Q000627:therapeutic use*; D010919:Placebos; D011122:Polyphosphates / Q000008:administration & dosage / Q000627:therapeutic use*; D012969:Sodium Fluoride / Q000008:administration & dosage / Q000627:therapeutic use; D013696:Temperature; D013997:Time Factors; D017001:Tooth Demineralization / Q000517:prevention & control*
chemical_list: D002327:Cariostatic Agents; D009067:Mouthwashes; D010919:Placebos; D011122:Polyphosphates; D012969:Sodium Fluoride; C009293:trimetaphosphoric acid; D005459:Fluorides