title: hESC-secreted proteins can be enriched for multiple regenerative therapies by heparin-binding.
abstract: This work builds upon our findings that proteins secreted by hESCs exhibit pro-regenerative activity, and determines that hESC-conditioned medium robustly enhances the proliferation of both muscle and neural progenitor cells. Importantly, this work establishes that it is the proteins that bind heparin which are responsible for the pro-myogenic effects of hESC-conditioned medium, and indicates that this strategy is suitable for enriching the potentially therapeutic factors. Additionally, this work shows that hESC-secreted proteins act independently of the mitogen FGF-2, and suggests that FGF-2 is unlikely to be a pro-aging molecule in the physiological decline of old muscle repair. Moreover, hESC-secreted factors improve the viability of human cortical neurons in an Alzheimer's disease (AD) model, suggesting that these factors can enhance the maintenance and regeneration of multiple tissues in the aging body.
authors.affiliation: Department of Bioengineering and California Institute for Quantitative Biosciences (QB3), UC Berkeley, Berkeley, CA 94720, USA.
authors.forename: Hanadie
authors.initials: H
authors.lastname: Yousef
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Conboy
authors.forename: Ju
authors.initials: J
authors.lastname: Li
authors.forename: Matthew
authors.initials: M
authors.lastname: Zeiderman
authors.forename: Tandis
authors.initials: T
authors.lastname: Vazin
authors.forename: Christina
authors.initials: C
authors.lastname: Schlesinger
authors.forename: David V
authors.initials: DV
authors.lastname: Schaffer
authors.forename: Irina M
authors.initials: IM
authors.lastname: Conboy
mesh_terms: D000375:Aging / Q000378:metabolism / Q000502:physiology; D000818:Animals; D017077:Culture Media, Conditioned / Q000737:chemistry / Q000494:pharmacology; D053595:Embryonic Stem Cells / Q000378:metabolism*; D016222:Fibroblast Growth Factor 2 / Q000235:genetics / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D006493:Heparin / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL; D024510:Muscle Development / Q000187:drug effects; D018482:Muscle, Skeletal / Q000293:injuries / Q000502:physiology; D009474:Neurons / Q000187:drug effects / Q000502:physiology; D032496:Satellite Cells, Skeletal Muscle / Q000166:cytology / Q000187:drug effects / Q000502:physiology
chemical_list: D017077:Culture Media, Conditioned; D016222:Fibroblast Growth Factor 2; D006493:Heparin