title: Rapid and highly sensitive detection of Enterovirus 71 by using nanogold-enhanced electrochemical impedance spectroscopy.
abstract: Enterovirus 71 (EV71) infection is an emerging infectious disease causing neurological complications and/or death within two to three days after the development of fever and rash. A low viral titre in clinical specimens makes the detection of EV71 difficult. Conventional approaches for detecting EV71 are time consuming, poorly sensitive, or complicated, and cannot be used effectively for clinical diagnosis. Furthermore, EV71 and Coxsackie virus A16 (CA16) may cross react in conventional assays. Therefore, a rapid, highly sensitive, specific, and user-friendly test is needed. We developed an EV71-specific nanogold-modified working electrode for electrochemical impedance spectroscopy in the detection of EV71. Our results show that EV71 can be distinguished from CA16, Herpes simplex virus, and lysozyme, with the modified nanogold electrode being able to detect EV71 in concentrations as low as 1 copy number/50 μl reaction volume, and the duration between sample preparation and detection being 11 min. This detection platform may have the potential for use in point-of-care diagnostics.
authors.affiliation: Department of Biological Science and Technology, National Chiao Tung University, Hsinchu, Taiwan.
authors.forename: Hsing-Yuan
authors.initials: HY
authors.lastname: Li
authors.forename: Shing-Hua
authors.initials: SH
authors.lastname: Tseng
authors.forename: Tsai-Mu
authors.initials: TM
authors.lastname: Cheng
authors.forename: Hsueh-Liang
authors.initials: HL
authors.lastname: Chu
authors.forename: Yu-Ning
authors.initials: YN
authors.lastname: Lu
authors.forename: Fang-Yu
authors.initials: FY
authors.lastname: Wang
authors.forename: Li-Yun
authors.initials: LY
authors.lastname: Tsai
authors.forename: Juo-Yu
authors.initials: JY
authors.lastname: Shieh
authors.forename: Jyh-Yuan
authors.initials: JY
authors.lastname: Yang
authors.forename: Chien-Chang
authors.initials: CC
authors.lastname: Juan
authors.forename: Lung-Chen
authors.initials: LC
authors.lastname: Tu
authors.forename: Chia-Ching
authors.initials: CC
authors.lastname: Chang
mesh_terms: D055910:Antibodies, Immobilized / Q000737:chemistry / Q000378:metabolism; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods*; D058266:Dielectric Spectroscopy / Q000379:methods*; D029821:Enterovirus A, Human / Q000276:immunology / Q000302:isolation & purification*; D006046:Gold / Q000737:chemistry*; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry*; D050997:Nanomedicine / Q000295:instrumentation / Q000379:methods; D012680:Sensitivity and Specificity
chemical_list: D055910:Antibodies, Immobilized; D006046:Gold