title: Influence of pulsed electromagnetic and pulsed vector magnetic potential field on the growth of tumor cells.
abstract: OBJECTIVE
  Tumor diseases cause 20% of deaths in Europe and they are the second most common cause of death and morbidity after cardiovascular diseases. Thus, tumor cells are target of many therapeutic strategies and tumor research is focused on searching more efficient and specific drugs as well as new therapeutic approaches. One of the areas of tumor research is an issue of external fields. In our work, we tested influence of a pulsed electromagnetic field (PEMF) and a hypothetic field of the pulsed vector magnetic potential (PVMP) on the growth of tumor cells; and further the possible growth inhibition effect of the PVMP.
  
  
  METHODS
  Both unipolar and bipolar PEMF fields of 5 mT and PVMP fields of 0 mT at frequencies of 15 Hz, 125 Hz and 625 Hz were tested on cancer cell lines derived from various types of tumors: CEM/C2 (acute lymphoblastic leukemia), SU-DHL-4 (B-cell lymphoma), COLO-320DM (colorectal adenocarcinoma), MDA-BM-468 (breast adenocarcinoma), and ZR-75-1 (ductal carcinoma). Cell morphology was observed, proliferation activity using WST assay was measured and simultaneous proportion of live, early apoptotic and dead cells was detected using flow cytometry.
  
  
  RESULTS
  A PEMF of 125 Hz and 625 Hz for 24 h-48 h increased proliferation activity in the 2 types of cancer cell lines used, i.e. COLO-320DM and ZR-75-1. In contrast, any of employed methods did not confirm a significant inhibitory effect of hypothetic PVMP field on tumor cells.
authors.affiliation: Masaryk Memorial Cancer Institute , Brno , Czech Republic .
authors.forename: Tomas
authors.initials: T
authors.lastname: Loja
authors.forename: Olga
authors.initials: O
authors.lastname: Stehlikova
authors.forename: Lukas
authors.initials: L
authors.lastname: Palko
authors.forename: Kamil
authors.initials: K
authors.lastname: Vrba
authors.forename: Ivan
authors.initials: I
authors.lastname: Rampl
authors.forename: Martin
authors.initials: M
authors.lastname: Klabusay
mesh_terms: D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000528:radiation effects; D002470:Cell Survival / Q000528:radiation effects; D004574:Electromagnetic Fields; D006801:Humans; D055909:Magnetic Field Therapy / Q000295:instrumentation / Q000379:methods*; D060526:Magnetic Fields
keywords: Cancer cell lines; cell viability; pulsed electromagnetic field (PEMF); pulsed vector magnetic potential (PVMP) field