title: Structure and gene cluster of the O-antigen of Escherichia coli O76.
abstract: The O-polysaccharide (O-antigen) of Escherichia coli O76 was studied by sugar analysis along with 1D and 2D (1)H,(13)C NMR spectroscopies. The following structure of the linear tetrasaccharide repeating unit was established: →4)-β-D-GlcpA-(1→4)-β-D-GalpNAc3Ac-(1→4)-α-D-GalpNAc-(1→3)-β-D-GalpNAc-(1→. The degree of O-acetylation of 4-substituted β-GalNAc residue is ~70%. The O-antigen gene cluster of E. coli O76 was sequenced. The functions of genes in the O-antigen gene cluster were tentatively assigned by comparison with sequences in the available databases and found to be in full agreement with the E. coli O76 O-antigen structure.
authors.affiliation: ND Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russian Federation. perepel@ioc.ac.ru
authors.forename: Andrei V
authors.initials: AV
authors.lastname: Perepelov
authors.forename: Quan
authors.initials: Q
authors.lastname: Wang
authors.forename: Sof'ya N
authors.initials: SN
authors.lastname: Senchenkova
authors.forename: Lu
authors.initials: L
authors.lastname: Feng
authors.forename: Alexander S
authors.initials: AS
authors.lastname: Shashkov
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.forename: Yuriy A
authors.initials: YA
authors.lastname: Knirel
mesh_terms: D000107:Acetylation; D000116:Acetylgalactosamine / Q000737:chemistry; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics; D001483:Base Sequence; D002240:Carbohydrate Sequence; D004926:Escherichia coli / Q000737:chemistry* / Q000235:genetics; D009682:Magnetic Resonance Spectroscopy; D058977:Molecular Sequence Annotation; D008969:Molecular Sequence Data; D005810:Multigene Family; D019081:O Antigens / Q000737:chemistry* / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D001426:Bacterial Proteins; D019081:O Antigens; D000116:Acetylgalactosamine
keywords: Bacterial polysaccharide structure; Escherichia coli; Lipopolysaccharide; O-Antigen; O-Antigen gene cluster