title: Solid phase assay measuring C4d deposition to determine complement fixation by HLA-specific antibodies.
abstract: The definition of HLA-specific antibodies in solid organ transplant patients is a necessary tool for recipient selection prior to transplantation and monitoring for rejection post transplant. Solid phase assays can detect both complement fixing and non-complement fixing HLA-specific antibodies. Here we describe a method for determining the presence of complement fixing HLA-specific antibodies using a sensitive solid phase assay.
authors.affiliation: Transplant Immunology, Harefield Hospital, Imperial College, Harefield, UK.
authors.forename: John D
authors.initials: JD
authors.lastname: Smith
authors.forename: Marlene L
authors.initials: ML
authors.lastname: Rose
mesh_terms: D000906:Antibodies / Q000276:immunology*; D000918:Antibody Specificity; D015935:Complement C4b / Q000276:immunology*; D003168:Complement Fixation Tests; D006084:Graft Rejection / Q000276:immunology; D006680:HLA Antigens / Q000276:immunology*; D006801:Humans; D008967:Molecular Biology / Q000379:methods; D010446:Peptide Fragments / Q000276:immunology*; D014180:Transplantation; D014184:Transplantation, Homologous
chemical_list: D000906:Antibodies; D006680:HLA Antigens; D010446:Peptide Fragments; D015935:Complement C4b; C032261:complement C4d