title: Targeting endogenous nuclear antigens by electrotransfer of monoclonal antibodies in living cells.
abstract: Antibodies are valuable tools for functional studies in vitro, but their use in living cells remains challenging because they do not naturally cross the cell membrane. Here, we present a simple and highly efficient method for the intracytoplasmic delivery of any antibody into cultured cells. By following the fate of monoclonal antibodies that bind to nuclear antigens, it was possible to image endogenous targets and to show that inhibitory antibodies are able to induce cell growth suppression or cell death. Our electrotransfer system allowed the cancer cells we studied to be transduced without loss of viability and may have applications for a variety of intracellular immuno-interventions.
authors.affiliation: Ecole Supérieure de Biotechnologie de Strasbourg, UMR 7242; CNRS/Université de Strasbourg, Illkirch, France.
authors.forename: Guillaume
authors.initials: G
authors.lastname: Freund
authors.forename: Annie-Paule
authors.initials: AP
authors.lastname: Sibler
authors.forename: Dominique
authors.initials: D
authors.lastname: Desplancq
authors.forename: Mustapha
authors.initials: M
authors.lastname: Oulad-Abdelghani
authors.forename: Marc
authors.initials: M
authors.lastname: Vigneron
authors.forename: Julian
authors.initials: J
authors.lastname: Gannon
authors.forename: Marc H
authors.initials: MH
authors.lastname: Van Regenmortel
authors.forename: Etienne
authors.initials: E
authors.lastname: Weiss
mesh_terms: D058846:Antibodies, Monoclonal, Murine-Derived / Q000737:chemistry / Q000276:immunology / Q000494:pharmacology; D034961:Antigens, Nuclear / Q000737:chemistry / Q000276:immunology / Q000378:metabolism; D017209:Apoptosis / Q000187:drug effects / Q000276:immunology; D016923:Cell Death / Q000187:drug effects; D006367:HeLa Cells; D006801:Humans; D009369:Neoplasms / Q000737:chemistry / Q000276:immunology / Q000378:metabolism
chemical_list: D058846:Antibodies, Monoclonal, Murine-Derived; D034961:Antigens, Nuclear
keywords: electroporation; intracellular delivery; intracellular imaging; living cells; monoclonal antibodies; protein interference