title: Poly(N-acryloylmorpholine): a simple hydrogel system for temporal and spatial control over cell adhesion.
abstract: N-acryloylmorpholine (NAM) was photo-polymerized to produce the homopolymer poly(N-acryloylmorpholine) (PNAM). PNAM behaves as a physical hydrogel in aqueous solvents, doubling its dry weight over a 2 h period before undergoing dissolution following a second order exponential decay profile. In vitro cellular experiments using mouse myoblasts showed that PNAM acts as an effective spatial cell barrier for 38 h, with slow migration of cells into the PNAM area occurring between 45 and 73 h after cell seeding. At 80 h myoblasts fully occupied the area initially blocked by PNAM. Immunofluorescent staining of myoblasts adjacent to PNAM showed normal cytoskeletal structure and well developed focal adhesions indicating limited PNAM toxicity. This study shows that PNAM is an easy to synthesize physical hydrogel that acts as a temporal and spatial barrier to cell adhesion.
authors.affiliation: Canniesburn Plastic Surgery Department, Royal Infirmary, 84 Castle Street, Glasgow, G4 0SF, United Kingdom; Centre for Cell Engineering, Institute for Molecular, Cell and Systems Biology, University of Glasgow, Glasgow, G12 8QQ, United Kingdom.
authors.forename: Mark
authors.initials: M
authors.lastname: Gorman
authors.forename: Ya Hua
authors.initials: YH
authors.lastname: Chim
authors.forename: Andrew
authors.initials: A
authors.lastname: Hart
authors.forename: Mathis O
authors.initials: MO
authors.lastname: Riehle
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Urquhart
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry*; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D002448:Cell Adhesion; D002460:Cell Line; D002465:Cell Movement; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry*; D051379:Mice; D032446:Myoblasts / Q000166:cytology*; D058105:Polymerization
chemical_list: D000180:Acrylic Resins; D001672:Biocompatible Materials; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; C015460:poly(acryloylmorpholine)
keywords: cell barrier; hydrogel; myoblasts; photo-polymerization; poly(N-acryloylmorpholine)