title: Determination of the myoglobin states in ground beef using non-invasive reflectance spectrometry and multivariate regression analysis.
abstract: Seventy-two samples of ground beef from M. semimembranosus of two 5 and two 1.5year old animals were prepared. Two types of fat tissues from either beef or pork were added to the ground beef. The samples were prepared to contain predominantly deoxymyoglobin (DMb), oxymyoglobin (OMb) and metmyoglobin (MMb) states on surfaces using selected methods based on chemical treatment (for MMb) and oxygen pressure packaging to induce the two other states. Reflectance spectra were measured on ground beef after three storage times. Partial least regression analysis was used to make calibration models of the desired myoglobin states. Validated models using leave-one-sample out cross validation gave, after correction and normalization, prediction errors of about 5%. Long term storage of ground beef was unsuitable for preparing pure MMb states due to gradual reduction of the pigment to DMb, presumably by bacteria.
authors.affiliation: Department of Chemistry, Biotechnology and Food Science, University of Life Sciences, P.O. Box 5003, NO-1432 Ås, Norway. milena.bjelanovic@umb.no
authors.forename: Milena
authors.initials: M
authors.lastname: Bjelanovic
authors.forename: Oddvin
authors.initials: O
authors.lastname: Sørheim
authors.forename: Erik
authors.initials: E
authors.lastname: Slinde
authors.forename: Eero
authors.initials: E
authors.lastname: Puolanne
authors.forename: Tomas
authors.initials: T
authors.lastname: Isaksson
authors.forename: Bjørg
authors.initials: B
authors.lastname: Egelandsdal
mesh_terms: D000273:Adipose Tissue; D000818:Animals; D002138:Calibration; D002417:Cattle; D003116:Color; D004032:Diet; D005511:Food Handling / Q000379:methods*; D005516:Food Microbiology; D018857:Food Packaging / Q000379:methods; D061353:Food Storage / Q000379:methods; D006801:Humans; D016018:Least-Squares Analysis; D008460:Meat / Q000032:analysis* / Q000382:microbiology; D015999:Multivariate Analysis; D018482:Muscle, Skeletal / Q000737:chemistry*; D009211:Myoglobin / Q000032:analysis* / Q000378:metabolism; D010084:Oxidation-Reduction; D010100:Oxygen; D010858:Pigmentation; D011312:Pressure; D013057:Spectrum Analysis / Q000379:methods; D013552:Swine
chemical_list: D009211:Myoglobin; D010100:Oxygen
keywords: Deoxymyoglobin; Metmyoglobin; Myoglobin states; Oxymyoglobin; Partial least square regression analysis; Spectroscopy