title: Impedimetric DNA biosensor based on a nanoporous alumina membrane for the detection of the specific oligonucleotide sequence of dengue virus.
abstract: A novel and integrated membrane sensing platform for DNA detection is developed based on an anodic aluminum oxide (AAO) membrane. Platinum electrodes (~50-100 nm thick) are coated directly on both sides of the alumina membrane to eliminate the solution resistance outside the nanopores. The electrochemical impedance technique is employed to monitor the impedance changes within the nanopores upon DNA binding. Pore resistance (Rp) linearly increases in response towards the increasing concentration of the target DNA in the range of 1 × 10⁻¹² to 1 × 10⁻⁶ M. Moreover, the biosensor selectively differentiates the complementary sequence from single base mismatched (MM-1) strands and non-complementary strands. This study reveals a simple, selective and sensitive method to fabricate a label-free DNA biosensor.
authors.affiliation: Division of Chemistry and Biological Chemistry, School of Physical and Mathematical Sciences, Nanyang Technological University, 21 Nanyang Link, Singapore 637371, Singapore.
authors.forename: Jiajia
authors.initials: J
authors.lastname: Deng
authors.forename: Chee-Seng
authors.initials: CS
authors.lastname: Toh
mesh_terms: D000537:Aluminum Oxide / Q000737:chemistry*; D020137:Base Pair Mismatch; D001483:Base Sequence; D015374:Biosensing Techniques; D015342:DNA Probes / Q000737:chemistry / Q000378:metabolism; D004279:DNA, Viral / Q000032:analysis* / Q000737:chemistry; D003716:Dengue Virus / Q000235:genetics*; D055664:Electrochemical Techniques; D004566:Electrodes; D058608:Nanopores; D010984:Platinum / Q000737:chemistry
chemical_list: D015342:DNA Probes; D004279:DNA, Viral; D010984:Platinum; D000537:Aluminum Oxide