title: In vitro effects of IL-17 on angiogenic properties of endothelial cells in relation to oxygen levels.
abstract: The aim of this study has been to elucidate how different oxygen levels impact the effects of Interleukin-17 (IL-17) on angiogenic properties of endothelial cells. Two endothelial cell lines, mouse MS-1 and human EA.hy 926, were grown in 20% and 3% O2 and their angiogenic abilities analyzed after IL-17 treatment: proliferation, apoptosis, migration and tubulogenesis. Expression of endothelial nitric oxide synthase (eNOS) and cyclooxygenase-2 (Cox-2) was also measured. Considering EA.hy 926 cell line, hypoxia alone reduced proliferation, survival and migration, but not their ability to form tubules. When cultured at 20% O2 , IL-17 stimulated proliferation, migration and tubulogenesis, whereas a hypoxic environment did not affect their migration and proliferation, but increased their survival and tubulogenic properties. Expression of eNOS and Cox-2 increased by both IL-17 and hypoxia, as well as with their combination. With the MS-1 cell line hypoxia did not affect proliferation, survival, migration and tubule formation. At 20% O2 , IL-17 did not alter their proliferation,but inhibited migration and stimulated tubule formation. At 3% O2 , only the stimulating effect of IL-17 on tubulogenesis was evident. The constitutive expression of eNOS was unaffected by oxygen concentrations or IL-17 supplementation, whereas both IL-17 and hypoxia upregulated Cox-2 expression. Thus the effects of IL-17 on the angiogenic properties of endothelial cells depend on both the cell line used and the oxygen concentration.
authors.affiliation: Laboratory for Experimental Hematology and Stem Cells, Institute for Medical Research, University of Belgrade, Dr. Subotića 4, P.O. Box 102, 11129, Belgrade, Serbia.
authors.forename: Jelena
authors.initials: J
authors.lastname: Krstić
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Jauković
authors.forename: Slavko
authors.initials: S
authors.lastname: Mojsilović
authors.forename: Ivana Okić
authors.initials: IO
authors.lastname: Ðorđević
authors.forename: Drenka
authors.initials: D
authors.lastname: Trivanović
authors.forename: Vesna
authors.initials: V
authors.lastname: Ilić
authors.forename: Juan F
authors.initials: JF
authors.lastname: Santibañez
authors.forename: Diana
authors.initials: D
authors.lastname: Bugarski
mesh_terms: D000818:Animals; D015687:Cell Hypoxia / Q000187:drug effects; D002460:Cell Line; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D051546:Cyclooxygenase 2 / Q000378:metabolism; D042783:Endothelial Cells / Q000166:cytology / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D006801:Humans; D020381:Interleukin-17 / Q000494:pharmacology*; D051379:Mice; D018919:Neovascularization, Physiologic / Q000187:drug effects*; D052250:Nitric Oxide Synthase Type III / Q000378:metabolism; D010100:Oxygen / Q000494:pharmacology*
chemical_list: D020381:Interleukin-17; D052250:Nitric Oxide Synthase Type III; D051546:Cyclooxygenase 2; D010100:Oxygen
keywords: EA.hy 926; IL-17; MS-1; angiogenesis; endothelial cells; hypoxia