title: Comparison of EDC and DMTMM efficiency in glycoconjugate preparation.
abstract: A series of mono- or disaccharide-protein conjugates were prepared by amide bond formation. Incorporation efficiency of the aminosaccharides on bovine serum albumin was determined by colorimetry and matrix assisted laser desorption/ionization mass spectrometry. Study compares two amide bond coupling agents, 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride and 4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride. Paper demonstrates a practical usefulness of natural bovine serum albumin succinylation. Large number of its carboxyl groups results in its superior reactivity towards simple aminosaccharides.
authors.affiliation: Institute of Chemistry, Slovak Academy of Sciences, Dúbravská cesta 9, Bratislava, SK-845 38, Slovakia. chempalo@savba.sk
authors.forename: Pavol
authors.initials: P
authors.lastname: Farkaš
authors.forename: Alžbeta
authors.initials: A
authors.lastname: Čížová
authors.forename: Slávka
authors.initials: S
authors.lastname: Bekešová
authors.forename: Slavomír
authors.initials: S
authors.lastname: Bystrický
mesh_terms: D000818:Animals; D002417:Cattle; D005022:Ethyldimethylaminopropyl Carbodiimide / Q000737:chemistry*; D006001:Glycoconjugates / Q000737:chemistry*; D012709:Serum Albumin / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry*
chemical_list: D006001:Glycoconjugates; D012709:Serum Albumin; C034877:succinylalbumin; D012710:Serum Albumin, Bovine; D005022:Ethyldimethylaminopropyl Carbodiimide
keywords: Glycoconjugate, Amide bond, Succinylated BSA