title: Asparagus byproducts as a new source of peroxidases.
abstract: Soluble peroxidase (POD) from asparagus byproducts was purified by ion exchange chromatographies, and its kinetic and catalytic properties were studied. The isoelectric point of the purified isoperoxidases was 9.1, and the optimum pH and temperature values were 4.0 and 25 °C, respectively. The cationic asparagus POD (CAP) midpoint inactivation temperature was 57 °C, which favors its use in industrial processes. The Km values of cationic asparagus POD for H₂O₂ and ABTS were 0.318 and 0.634 mM, respectively. The purified CAP is economically obtained from raw materials using a simple protocol and possesses features that make it advantageous for the potential use of this enzyme in a large number of processes with demonstrated requirements of thermostable POD. The results indicate that CAP can be used as a potential candidate for removing phenolic contaminants.
authors.affiliation: Phytochemicals and Food Quality Group, Instituto de la Grasa (CSIC), 41014 Seville, Spain.
authors.forename: Sara
authors.initials: S
authors.lastname: Jaramillo-Carmona
authors.forename: Sergio
authors.initials: S
authors.lastname: Lopez
authors.forename: Sara
authors.initials: S
authors.lastname: Vazquez-Castilla
authors.forename: Rocio
authors.initials: R
authors.lastname: Rodriguez-Arcos
authors.forename: Ana
authors.initials: A
authors.lastname: Jimenez-Araujo
authors.forename: Rafael
authors.initials: R
authors.lastname: Guillen-Bejarano
mesh_terms: D027761:Asparagus Plant / Q000201:enzymology*; D001673:Biodegradation, Environmental; D005525:Food-Processing Industry / Q000191:economics; D007220:Industrial Waste / Q000032:analysis* / Q000191:economics; D010544:Peroxidases / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D010940:Plant Proteins / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D013030:Spain; D062065:Wastewater / Q000737:chemistry; D014874:Water Pollutants, Chemical / Q000378:metabolism; D018508:Water Purification / Q000191:economics
chemical_list: D007220:Industrial Waste; D010544:Peroxidases; D010940:Plant Proteins; D062065:Wastewater; D014874:Water Pollutants, Chemical