title: Immunoenhancement effect of rehmannia glutinosa polysaccharide on lymphocyte proliferation and dendritic cell.
abstract: The aim of this study is to investigate immunomodulatory effect of rehmannia glutinosa polysaccharide (RGP) on murine splenic lymphocyte and bone marrow derived dendritic cells (DCs). Splenic lymphocytes obtained from mice were co-cultured with RGP for 48 h and then harvested for analyzing with MTT method. The cytokine production of T lymphocytes was measured by ELISA. Effects of RGP treatment on DCs were investigated and assessed by MTT method. The results showed RGP significantly stimulated lymphocyte proliferation and the growth rate of T cell was more significant. The IL-2 and IFN-γ production of T lymphocyte were significantly upregulated after being stimulated with RGP. DCs stimulating on proliferation of T cells and the ability of antigen presenting of DCs have been enhanced under the stimulation of RGP. In conclusion, these findings provided valuable information that RGP possessed strong immunoenhancement activity, which provided the theoretical basis for the further experiment.
authors.affiliation: Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Yee
authors.initials: Y
authors.lastname: Huang
authors.forename: Chunmao
authors.initials: C
authors.lastname: Jiang
authors.forename: Yuanliang
authors.initials: Y
authors.lastname: Hu
authors.forename: Xiaojuan
authors.initials: X
authors.lastname: Zhao
authors.forename: Cheng
authors.initials: C
authors.lastname: Shi
authors.forename: Yun
authors.initials: Y
authors.lastname: Yu
authors.forename: Cui
authors.initials: C
authors.lastname: Liu
authors.forename: Yang
authors.initials: Y
authors.lastname: Tao
authors.forename: Huarong
authors.initials: H
authors.lastname: Pan
authors.forename: Yibo
authors.initials: Y
authors.lastname: Feng
authors.forename: Jiaguo
authors.initials: J
authors.lastname: Liu
authors.forename: Yi
authors.initials: Y
authors.lastname: Wu
authors.forename: Deyun
authors.initials: D
authors.lastname: Wang
mesh_terms: D000818:Animals; D017951:Antigen Presentation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects*; D003713:Dendritic Cells / Q000187:drug effects* / Q000276:immunology; D007155:Immunologic Factors / Q000494:pharmacology*; D007371:Interferon-gamma / Q000235:genetics / Q000378:metabolism; D007376:Interleukin-2 / Q000235:genetics / Q000378:metabolism; D051379:Mice; D011134:Polysaccharides / Q000494:pharmacology*; D032267:Rehmannia / Q000737:chemistry*; D013601:T-Lymphocytes / Q000187:drug effects* / Q000378:metabolism
chemical_list: D007155:Immunologic Factors; D007376:Interleukin-2; D011134:Polysaccharides; D007371:Interferon-gamma