title: The expression of intronic miRNAs, miR-483 and miR-483*, and their host gene, Igf2, in murine osteoarthritis cartilage.
abstract: MicroRNAs (miRNAs) have been shown to be important regulators in the pathogenesis of osteoarthritis (OA). The objective of this study was to determine the expression levels of miR-483, miR-483*, their host gene (Igf2) and other cytokines in a murine model of OA. The expression of miR-483 was significantly up-regulated in old mouse and in all of the operation groups, particularly the group assessed 1 week after surgery. The expression of miR-483* was significantly increased in the old mouse group and the group assessed 5 weeks after surgery. The expression of miR-483 was negatively correlated with the expression of (mRNA) Bmp7 and TgfB and positively correlated with Mmp13 by Pearson correlation analysis, while miR-483* was positively correlated with Il1B. Surprisingly, there was no correlation between the expression of either miR-483 or miR-483* and Igf2. This study shows that the expression of miR-483 and miR-483* is up-regulated in murine OA. These data suggest that miR-483 and miR-483* may play critical roles in early and later pathogenesis of OA, respectively, without the involvement of their host gene Igf2.
authors.affiliation: The First Affiliated Hospital of Harbin Medical University, Harbin, China.
authors.forename: Yuebin
authors.initials: Y
authors.lastname: Qi
authors.forename: Ning
authors.initials: N
authors.lastname: Ma
authors.forename: Feng
authors.initials: F
authors.lastname: Yan
authors.forename: Zhange
authors.initials: Z
authors.lastname: Yu
authors.forename: Guodong
authors.initials: G
authors.lastname: Wu
authors.forename: Yu
authors.initials: Y
authors.lastname: Qiao
authors.forename: Dong
authors.initials: D
authors.lastname: Han
authors.forename: Ying
authors.initials: Y
authors.lastname: Xiang
authors.forename: Fuyuan
authors.initials: F
authors.lastname: Li
authors.forename: Wenbo
authors.initials: W
authors.lastname: Wang
authors.forename: Xu
authors.initials: X
authors.lastname: Gao
mesh_terms: D000818:Animals; D055419:Bone Morphogenetic Protein 7 / Q000235:genetics / Q000378:metabolism; D002356:Cartilage / Q000378:metabolism* / Q000473:pathology*; D016207:Cytokines / Q000235:genetics / Q000378:metabolism; D004195:Disease Models, Animal; D007150:Immunohistochemistry; D007335:Insulin-Like Growth Factor II / Q000235:genetics* / Q000378:metabolism; D053583:Interleukin-1beta / Q000235:genetics / Q000378:metabolism; D008297:Male; D053509:Matrix Metalloproteinase 13 / Q000235:genetics / Q000378:metabolism; D051379:Mice; D035683:MicroRNAs / Q000235:genetics*; D010003:Osteoarthritis / Q000235:genetics* / Q000378:metabolism; D016212:Transforming Growth Factor beta / Q000235:genetics / Q000378:metabolism
chemical_list: D055419:Bone Morphogenetic Protein 7; D016207:Cytokines; D053583:Interleukin-1beta; D035683:MicroRNAs; C548913:Mirn483 microRNA, mouse; D016212:Transforming Growth Factor beta; D007335:Insulin-Like Growth Factor II; D053509:Matrix Metalloproteinase 13
keywords: Cartilage; IGF2; MicroRNA; Osteoarthritis; miR-483