title: Comparative assessment of iridium oxide and platinum alloy wires using an in vitro glial scar assay.
abstract: The long-term effect of chronically implanted electrodes is the formation of a glial scar. Therefore, it is imperative to assess the biocompatibility of materials before employing them in neural electrode fabrication. Platinum alloy and iridium oxide have been identified as good candidates as neural electrode biomaterials due to their mechanical and electrical properties, however, effect of glial scar formation for these two materials is lacking. In this study, we applied a glial scarring assay to observe the cellular reactivity to platinum alloy and iridium oxide wires in order to assess the biocompatibility based on previously defined characteristics. Through real-time PCR, immunostaining and imaging techniques, we will advance the understanding of the biocompatibility of these materials. Results of this study demonstrate iridium oxide wires exhibited a more significant reactive response as compared to platinum alloy wires. Cells cultured with platinum alloy wires had less GFAP gene expression, lower average GFAP intensity, and smaller glial scar thickness. Collectively, these results indicated that platinum alloy wires were more biocompatible than the iridium oxide wires.
authors.affiliation: Department of Biomedical Engineering, Wayne State University, Detroit, MI, USA.
authors.forename: Evon S
authors.initials: ES
authors.lastname: Ereifej
authors.forename: Saida
authors.initials: S
authors.lastname: Khan
authors.forename: Golam
authors.initials: G
authors.lastname: Newaz
authors.forename: Jinsheng
authors.initials: J
authors.lastname: Zhang
authors.forename: Gregory W
authors.initials: GW
authors.lastname: Auner
authors.forename: Pamela J
authors.initials: PJ
authors.lastname: VandeVord
mesh_terms: D000497:Alloys; D000818:Animals; D001681:Biological Assay; D002921:Cicatrix / Q000139:chemically induced* / Q000473:pathology; D005786:Gene Expression Regulation / Q000187:drug effects; D007495:Iridium / Q000009:adverse effects*; D008422:Materials Testing / Q000379:methods*; D009457:Neuroglia / Q000473:pathology*; D009474:Neurons / Q000187:drug effects; D010984:Platinum / Q000009:adverse effects* / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013194:Staining and Labeling
chemical_list: D000497:Alloys; C044458:iridium oxide; D007495:Iridium; D010984:Platinum