title: Tetramer staining for the detection of HLA-specific B cells.
abstract: HLA-specific B cells can be identified, quantified, and isolated after staining with HLA tetramers. Quantification of these B cells can in turn identify individuals who are sensitized to HLA antigens and the isolation of these cells facilitates a variety of experimental investigations.
authors.affiliation: Immunogenetics Laboratory, Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
authors.forename: Donna P
authors.initials: DP
authors.lastname: Lucas
authors.forename: Mary S
authors.initials: MS
authors.lastname: Leffell
authors.forename: Andrea A
authors.initials: AA
authors.lastname: Zachary
mesh_terms: D000906:Antibodies / Q000276:immunology; D000918:Antibody Specificity; D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology*; D006680:HLA Antigens / Q000276:immunology*; D006801:Humans; D008967:Molecular Biology / Q000379:methods*; D055503:Protein Multimerization / Q000276:immunology*
chemical_list: D000906:Antibodies; D006680:HLA Antigens