title: Culturing primary human osteoblasts on electrospun poly(lactic-co-glycolic acid) and poly(lactic-co-glycolic acid)/nanohydroxyapatite scaffolds for bone tissue engineering.
abstract: In this work, we fabricated polymeric fibrous scaffolds for bone tissue engineering using primary human osteoblasts (HOB) as the model cell. By employing one simple approach, electrospinning, we produced poly(lactic-co-glycolic acid) (PLGA) scaffolds with different topographies including microspheres, beaded fibers, and uniform fibers, as well as the PLGA/nanohydroxyapatite (nano-HA) composite scaffold. The bone-bonding ability of electrospun scaffolds was investigated by using simulated body fluid (SBF) solution, and the nano-HA in PLGA/nano-HA composite scaffold can significantly enhance the formation of the bonelike apatites. Furthermore, we carried out in vitro experiments to test the performance of electrospun scaffolds by utilizing both mouse preosteoblast cell line (MC 3T3 E1) and HOB. Results including cell viability, alkaline phosphatase (ALP) activity, and osteocalcin concentration demonstrated that the PLGA/nano-HA fibers can promote the proliferation of HOB efficiently, indicating that it is a promising scaffold for human bone repair.
authors.forename: Mengmeng
authors.initials: M
authors.lastname: Li
authors.forename: Wenwen
authors.initials: W
authors.lastname: Liu
authors.forename: Jiashu
authors.initials: J
authors.lastname: Sun
authors.forename: Yunlei
authors.initials: Y
authors.lastname: Xianyu
authors.forename: Jidong
authors.initials: J
authors.lastname: Wang
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: Wenfu
authors.initials: W
authors.lastname: Zheng
authors.forename: Deyong
authors.initials: D
authors.lastname: Huang
authors.forename: Shiyu
authors.initials: S
authors.lastname: Di
authors.forename: Yun-Ze
authors.initials: YZ
authors.lastname: Long
authors.forename: Xingyu
authors.initials: X
authors.lastname: Jiang
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D001846:Bone Development; D049109:Cell Proliferation; D002470:Cell Survival; D017886:Durapatite / Q000737:chemistry*; D006801:Humans; D019344:Lactic Acid / Q000737:chemistry*; D051379:Mice; D010006:Osteoblasts / Q000166:cytology* / Q000378:metabolism; D015675:Osteocalcin / Q000378:metabolism; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D061251:Primary Cell Culture; D023822:Tissue Engineering / Q000295:instrumentation* / Q000379:methods; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D015675:Osteocalcin; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D017886:Durapatite