title: OT-II TCR transgenic mice fail to produce anti-ovalbumin antibodies upon vaccination.
abstract: OT-II mice were evaluated as a transgenic strain-specific model to assess T-cell help for B-cell responses. OT-II CD4(+) T-cells express transgenic OVA-specific αβ-TCRs. This high frequency of antigen-specific helper T-lymphocytes may augment induction of B-cell responses. Unexpectedly, OT-II mice did not produce OVA-specific antibodies after intranasal immunization. However, B-cells expressed normal antigen-presenting function in vitro for activation of OVA-specific T-cell responses. These OT-II T-cell responses produced a Th1-type cytokine profile with significantly reduced Th2 or Th17 responses. These data suggest that OT-II B-cells are not defective as APCs, however, downstream antibody responses are abrogated in this transgenic strain.
authors.affiliation: Graduate Program in Immunology, University of Michigan Medical School, Ann Arbor, MI 48109, USA. sheleung@umich.edu
authors.forename: Shelly
authors.initials: S
authors.lastname: Leung
authors.forename: Douglas
authors.initials: D
authors.lastname: Smith
authors.forename: Andrzej
authors.initials: A
authors.lastname: Myc
authors.forename: Jingga
authors.initials: J
authors.lastname: Morry
authors.forename: James R
authors.initials: JR
authors.lastname: Baker
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology / Q000378:metabolism; D002478:Cells, Cultured; D002772:Cholera Toxin / Q000276:immunology; D018920:Coculture Techniques; D016207:Cytokines / Q000276:immunology / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D010047:Ovalbumin / Q000276:immunology*; D011948:Receptors, Antigen, T-Cell / Q000235:genetics / Q000276:immunology*; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology / Q000378:metabolism; D018417:Th1 Cells / Q000276:immunology / Q000378:metabolism; D014611:Vaccination / Q000379:methods*
chemical_list: D000906:Antibodies; D016207:Cytokines; D007074:Immunoglobulin G; D011948:Receptors, Antigen, T-Cell; D010047:Ovalbumin; D002772:Cholera Toxin
keywords: APC; B-cell; Helper T-lymphocyte; Intranasal vaccine; T-B-cell interaction; Th1; Th17; Th2