title: Four monoclonal antibodies against capsular polysaccharides of Neisseria meningitidis serogroups A, C, Y and W135: its application in identity tests.
abstract: Murine hybridoma monoclonal antibodies (MAbs) were produced against the capsular polysaccharide (CPs) of serogroups A, C, W135 and Y meningococci (MenA, MenC, MenW, MenY) in order to develop immunological reagents for the identification of meningococcal polysaccharides. Each serogroup-specific MAb reacted with the CPs from its homologous serogroup only and did not react with CPs from the other three serogroups. The affinity constant (Ka) of the four MAbs measured by non-competitive ELISA was 6.62 × 10(9), 2.76 × 10(9), 1.48 × 10(9) and 3.8 × 10(9) M(-1) for MenA, MenC, MenW and MenY MAbs respectively. The application of these MAbs for identity tests was demonstrated by their abilities to correctly identify the CPs from serogroups A, C, W135 and Y in meningococcal CPs-based vaccines through ELISA. The MAbs obtained in this work are a very valuable set of tools for study meningococcal polysaccharides vaccines.
authors.affiliation: Finlay Institute, Calle 27, No. 19805, La Lisa, A.P. 16017, Cod. 11600 Havana, Cuba. freyes@finlay.edu.cu
authors.forename: Fátima
authors.initials: F
authors.lastname: Reyes
authors.forename: Nevis
authors.initials: N
authors.lastname: Amin
authors.forename: Oscar
authors.initials: O
authors.lastname: Otero
authors.forename: Alicia
authors.initials: A
authors.lastname: Aguilar
authors.forename: Maribel
authors.initials: M
authors.lastname: Cuello
authors.forename: Yolanda
authors.initials: Y
authors.lastname: Valdés
authors.forename: Luis G
authors.initials: LG
authors.lastname: García
authors.forename: Daniel
authors.initials: D
authors.lastname: Cardoso
authors.forename: Frank
authors.initials: F
authors.lastname: Camacho
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000737:chemistry / Q000276:immunology; D058846:Antibodies, Monoclonal, Murine-Derived / Q000737:chemistry / Q000276:immunology; D000918:Antibody Specificity; D016667:Bacterial Capsules / Q000737:chemistry / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D051379:Mice; D038381:Neisseria meningitidis, Serogroup A / Q000737:chemistry / Q000276:immunology; D038542:Neisseria meningitidis, Serogroup C / Q000737:chemistry / Q000276:immunology; D038582:Neisseria meningitidis, Serogroup W-135 / Q000737:chemistry / Q000276:immunology; D038581:Neisseria meningitidis, Serogroup Y / Q000737:chemistry / Q000276:immunology; D011135:Polysaccharides, Bacterial / Q000737:chemistry / Q000276:immunology
chemical_list: D000907:Antibodies, Bacterial; D058846:Antibodies, Monoclonal, Murine-Derived; D011135:Polysaccharides, Bacterial
keywords: Capsular polysaccharides; ELISA; Identity tests; Monoclonal antibodies; Quality control; Vaccines