title: Development and validation of a HPTLC method for simultaneous estimation of lornoxicam and thiocolchicoside in combined dosage form.
abstract: OBJECTIVE
  To develop a simple, precise, rapid and accurate HPTLC method for the simultaneous estimation of Lornoxicam (LOR) and Thiocolchicoside (THIO) in bulk and pharmaceutical dosage forms.
  
  
  METHODS
  The separation of the active compounds from pharmaceutical dosage form was carried out using methanol:chloroform:water (9.6:0.2:0.2 v/v/v) as the mobile phase and no immiscibility issues were found. The densitometric scanning was carried out at 377 nm. The method was validated for linearity, accuracy, precision, LOD (Limit of Detection), LOQ (Limit of Quantification), robustness and specificity.
  
  
  RESULTS
  The Rf values (±SD) were found to be 0.84 ± 0.05 for LOR and 0.58 ± 0.05 for THIO. Linearity was obtained in the range of 60-360 ng/band for LOR and 30-180 ng/band for THIO with correlation coefficients r(2) = 0.998 and 0.999, respectively. The percentage recovery for both the analytes was in the range of 98.7-101.2 %.
  
  
  CONCLUSIONS
  The proposed method was optimized and validated as per the ICH guidelines.
authors.affiliation: MAAER's Maharashtra Institute of Pharmacy, MIT Campus, Road, Kothrud, Pune, India.
authors.forename: Madhusmita
authors.initials: M
authors.lastname: Sahoo
authors.forename: Pratima
authors.initials: P
authors.lastname: Syal
authors.forename: Asawaree A
authors.initials: AA
authors.lastname: Hable
authors.forename: Rahul P
authors.initials: RP
authors.lastname: Raut
authors.forename: Vishnu P
authors.initials: VP
authors.lastname: Choudhari
authors.forename: Bhanudas S
authors.initials: BS
authors.lastname: Kuchekar
keywords: Densiotometry; HPTLC; lornoxicam; method validation; thiocolchicoside