title: A single strain of Clostridium butyricum induces intestinal IL-10-producing macrophages to suppress acute experimental colitis in mice.
abstract: Imbalance in gut bacterial composition provokes host proinflammatory responses causing diseases such as colitis. Colonization with a mixture of Clostridium species from clusters IV and XIVa was shown to suppress colitis through the induction of IL-10-producing regulatory T (Treg) cells. We demonstrate that a distinct Clostridium strain from cluster I, Clostridium butyricum (CB), prevents acute experimental colitis in mice through induction of IL-10, an anti-inflammatory cytokine. However, while CB treatment had no effect on IL-10 production by T cells, IL-10-producing F4/80(+)CD11b(+)CD11c(int) macrophages accumulated in the inflamed mucosa after CB treatment. CB directly triggered IL-10 production by intestinal macrophages in inflamed mucosa via the TLR2/MyD88 pathway. The colitis-preventing effect of CB was negated in macrophage-specific IL-10-deficient mice, suggesting that induction of IL-10 by intestinal macrophages is crucial for the probiotic action of CB. Collectively, CB promotes IL-10 production by intestinal macrophages in inflamed mucosa, thereby preventing experimental colitis in mice.
authors.affiliation: Division of Gastroenterology and Hepatology, Department of Internal Medicine, Keio University School of Medicine, Tokyo 160-8582, Japan.
authors.forename: Atsushi
authors.initials: A
authors.lastname: Hayashi
authors.forename: Toshiro
authors.initials: T
authors.lastname: Sato
authors.forename: Nobuhiko
authors.initials: N
authors.lastname: Kamada
authors.forename: Yohei
authors.initials: Y
authors.lastname: Mikami
authors.forename: Katsuyoshi
authors.initials: K
authors.lastname: Matsuoka
authors.forename: Tadakazu
authors.initials: T
authors.lastname: Hisamatsu
authors.forename: Toshifumi
authors.initials: T
authors.lastname: Hibi
authors.forename: Axel
authors.initials: A
authors.lastname: Roers
authors.forename: Hideo
authors.initials: H
authors.lastname: Yagita
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Ohteki
authors.forename: Akihiko
authors.initials: A
authors.lastname: Yoshimura
authors.forename: Takanori
authors.initials: T
authors.lastname: Kanai
mesh_terms: D000818:Animals; D003015:Clostridium Infections / Q000276:immunology* / Q000382:microbiology / Q000517:prevention & control; D046972:Clostridium butyricum / Q000276:immunology*; D003092:Colitis / Q000276:immunology* / Q000382:microbiology / Q000517:prevention & control; D004195:Disease Models, Animal; D016753:Interleukin-10 / Q000378:metabolism*; D007413:Intestinal Mucosa / Q000276:immunology; D008264:Macrophages / Q000276:immunology* / Q000382:microbiology*; D051379:Mice; D018345:Mice, Knockout
chemical_list: C556062:IL10 protein, mouse; D016753:Interleukin-10