title: Robust structural analysis of native biological macromolecules from multi-crystal anomalous diffraction data.
abstract: Structure determinations for biological macromolecules that have no known structural antecedents typically involve the incorporation of heavier atoms than those found natively in biological molecules. Currently, selenomethionyl proteins analyzed using single- or multi-wavelength anomalous diffraction (SAD or MAD) data predominate for such de novo analyses. Naturally occurring metal ions such as zinc or iron often suffice in MAD or SAD experiments, and sulfur SAD has been an option since it was first demonstrated using crambin 30 years ago; however, SAD analyses of structures containing only light atoms (Zmax ≤ 20) have not been common. Here, robust procedures for enhancing the signal to noise in measurements of anomalous diffraction by combining data collected from several crystals at a lower than usual X-ray energy are described. This multi-crystal native SAD method was applied in five structure determinations, using between five and 13 crystals to determine substructures of between four and 52 anomalous scatterers (Z ≤ 20) and then the full structures ranging from 127 to 1200 ordered residues per asymmetric unit at resolutions from 2.3 to 2.8 Å. Tests were devised to assure that all of the crystals used were statistically equivalent. Elemental identities for Ca, Cl, S, P and Mg were proven by f'' scattering-factor refinements. The procedures are robust, indicating that truly routine structure determination of typical native macromolecules is realised. Synchrotron beamlines that are optimized for low-energy X-ray diffraction measurements will facilitate such direct structural analysis.
authors.affiliation: New York Structural Biology Center, NSLS X4, Building 725, Brookhaven National Laboratory, Upton, NY 11973, USA.
authors.forename: Qun
authors.initials: Q
authors.lastname: Liu
authors.forename: Qinglian
authors.initials: Q
authors.lastname: Liu
authors.forename: Wayne A
authors.initials: WA
authors.lastname: Hendrickson
mesh_terms: D000255:Adenosine Triphosphate / Q000737:chemistry / Q000378:metabolism; D018360:Crystallography, X-Ray / Q000295:instrumentation / Q000379:methods*; D003625:Data Collection / Q000379:methods*; D001330:Electronic Data Processing / Q000379:methods*; D029968:Escherichia coli Proteins / Q000737:chemistry* / Q000378:metabolism; D058851:GPI-Linked Proteins / Q000737:chemistry / Q000378:metabolism; D018840:HSP70 Heat-Shock Proteins / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D046911:Macromolecular Substances / Q000737:chemistry*; D026901:Membrane Transport Proteins / Q000737:chemistry / Q000378:metabolism; D008958:Models, Molecular; D009419:Nerve Tissue Proteins / Q000737:chemistry / Q000378:metabolism; D000075385:Netrins; D011487:Protein Conformation; D012645:Selenomethionine / Q000378:metabolism; D017356:Synchrotrons / Q000295:instrumentation*
chemical_list: D029968:Escherichia coli Proteins; D058851:GPI-Linked Proteins; D018840:HSP70 Heat-Shock Proteins; D046911:Macromolecular Substances; D026901:Membrane Transport Proteins; C463083:NTNG2 protein, human; D009419:Nerve Tissue Proteins; D000075385:Netrins; D000255:Adenosine Triphosphate; D012645:Selenomethionine; C063842:dnaK protein, E coli
keywords: anomalous scattering; multiple crystals; phase determination; sulfur SAD