title: [Effect of mutations changing the antigenic specificity on the receptor-binding activity of the influenza virus hemagglutinin of H1 and H5 subtypes].
abstract: The influenza virus hemagglutinin (HA) is an envelope virus glycoprotein responsible for the attachment of the virus particles to cells via binding terminal sialic acid residues of cell surface oligosaccharides. In our previous works on influenza A virus escape mutants, that is, mutants resistant to the neutralization effect of monoclonal antibodies, we encountered amino acid changes in the vicinity of receptor-binding pocket of the HA. In this work the degree of the affinity to both alpha-2, -3, and alpha-2, -6, -sialoglycoconjugates was assessed for escape mutants of influenza H1 and H5 viruses. The data demonstrate that the decrease of the positive electrostatic charge of the HA molecule surface resulting from amino acid changes conferring resistance to monoclonal antibodies may lead to a lowering of the affinity to sialic acid-containing analogs of cell receptors. The results are discussed in the context of the evolution of HA in natural circulation of H1 and H5 influenza viruses.
authors.forename: T A
authors.initials: TA
authors.lastname: Timofeeva
authors.forename: A V
authors.initials: AV
authors.lastname: Ignat'eva
authors.forename: I A
authors.initials: IA
authors.lastname: Rudneva
authors.forename: L V
authors.initials: LV
authors.lastname: Mochalova
authors.forename: N V
authors.initials: NV
authors.lastname: Bovin
authors.forename: N V
authors.initials: NV
authors.lastname: Kaverin
mesh_terms: D019943:Amino Acid Substitution; D000818:Animals; D058846:Antibodies, Monoclonal, Murine-Derived / Q000737:chemistry / Q000276:immunology; D000914:Antibodies, Viral / Q000737:chemistry / Q000276:immunology; D002642:Chick Embryo; D019143:Evolution, Molecular; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000235:genetics / Q000276:immunology; D057131:Immune Evasion / Q000502:physiology; D053118:Influenza A Virus, H1N1 Subtype / Q000235:genetics / Q000276:immunology; D053124:Influenza A Virus, H5N1 Subtype / Q000235:genetics / Q000276:immunology; D020125:Mutation, Missense; D019158:N-Acetylneuraminic Acid
chemical_list: D058846:Antibodies, Monoclonal, Murine-Derived; D000914:Antibodies, Viral; C543345:H1N1 virus hemagglutinin; D019267:Hemagglutinin Glycoproteins, Influenza Virus; C109963:hemagglutinin, avian influenza A virus; D019158:N-Acetylneuraminic Acid