title: Serum antioxidative enzymes levels and oxidative stress products in age-related cataract patients.
abstract: OBJECTIVE
  To investigate the activity of antioxidative enzymes and the products of oxidative stress in patients with age-related cataracts and compare the findings with those in healthy control subjects.
  
  
  METHODS
  Sixty patients with age-related cataract and sixty healthy controls of matched age and gender were included in this study. Serum samples were obtained to detect the antioxidative enzymes of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px), and oxidation degradation products of malondialdehyde (MDA), 4-hydroxynonenal (4-HNE), conjugated diene (CD), advanced oxidation protein products (AOPP), protein carbonyl (PC), and 8-hydroxydeoxyguanosine (8-OHdG).
  
  
  RESULTS
  Serum SOD, GSH-Px, and CAT activities in cataract group were significantly decreased as compared to the control subjects (P < 0.05). The levels of MDA, 4-HNE, and CD in cataract patients were significantly higher than those in the control subjects (P < 0.05, P < 0.01). Cataract patients had higher levels of 8-OHdG, AOPP, and PC with respect to the comparative group of normal subjects (P < 0.01). And there was no statistical significance in concentration of antioxidative enzymes and oxidative stress products in patients with different subtype cataract.
  
  
  CONCLUSIONS
  Oxidative stress is an important risk factor in the development of age-related cataract, and augmentation of the antioxidant defence systems may be of benefit to prevent or delay cataractogenesis.
authors.affiliation: Clinical Labororatory, The First Affiliated Hospital of Harbin Medical University, Harbin 150081, China.
authors.forename: Dong
authors.initials: D
authors.lastname: Chang
authors.forename: Xuefei
authors.initials: X
authors.lastname: Zhang
authors.forename: Shengzhong
authors.initials: S
authors.lastname: Rong
authors.forename: Qian
authors.initials: Q
authors.lastname: Sha
authors.forename: Peipei
authors.initials: P
authors.lastname: Liu
authors.forename: Tao
authors.initials: T
authors.lastname: Han
authors.forename: Hongzhi
authors.initials: H
authors.lastname: Pan
mesh_terms: D000375:Aging / Q000097:blood*; D000975:Antioxidants / Q000378:metabolism*; D016022:Case-Control Studies; D002374:Catalase / Q000097:blood*; D002386:Cataract / Q000097:blood* / Q000201:enzymology / Q000473:pathology; D004249:DNA Damage; D005260:Female; D005979:Glutathione Peroxidase / Q000097:blood*; D006801:Humans; D015227:Lipid Peroxidation; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D008875:Middle Aged; D018384:Oxidative Stress; D013482:Superoxide Dismutase / Q000097:blood*
chemical_list: D000975:Antioxidants; D008315:Malondialdehyde; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase