title: Effects of submerged macrophytes on the abundance and community composition of ammonia-oxidizing prokaryotes in a eutrophic lake.
abstract: Abundances and community compositions of ammonia-oxidizing archaea (AOA) and ammonia-oxidizing bacteria (AOB) in unvegetated sediment and the rhizosphere sediments of three submerged macrophytes (Ceratophyllum demersum, Vallisneria spinulosa, and Potamogeton crispus) were investigated in a large, eutrophic freshwater lake, Lake Taihu. Abundances of archaeal ammonia monooxygenase alpha-subunit (amoA) gene (from 6.56 × 10(6) copies to 1.06 × 10(7) copies per gram of dry sediment) were higher than those of bacterial amoA (from 6.13 × 10(5) to 3.21 × 10(6) copies per gram of dry sediment) in all samples. Submerged macrophytes exhibited no significant effect on the abundance and diversity of archaeal amoA gene. C. demersum and V. spinulosa increased the abundance and diversity of bacterial amoA gene in their rhizosphere sediment. However, the diversity of bacterial amoA gene in the rhizosphere sediments of P. crispus was decreased. The data obtained in this study would be helpful to elucidate the roles of submerged macrophytes involved in the nitrogen cycling of eutrophic lake ecosystems.
authors.affiliation: State Key Laboratory of Hydrology-Water Resources and Hydraulic Engineering, Hohai University, Nanjing, 210098, China.
authors.forename: Da-yong
authors.initials: DY
authors.lastname: Zhao
authors.forename: Juan
authors.initials: J
authors.lastname: Luo
authors.forename: Jin
authors.initials: J
authors.lastname: Zeng
authors.forename: Meng
authors.initials: M
authors.lastname: Wang
authors.forename: Wen-ming
authors.initials: WM
authors.lastname: Yan
authors.forename: Rui
authors.initials: R
authors.lastname: Huang
authors.forename: Qinglong L
authors.initials: QL
authors.lastname: Wu
mesh_terms: D000641:Ammonia / Q000378:metabolism*; D001105:Archaea / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D001419:Bacteria / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D001673:Biodegradation, Environmental; D017753:Ecosystem; D005068:Eutrophication; D019766:Genes, Archaeal; D005798:Genes, Bacterial; D060106:Lakes / Q000737:chemistry / Q000382:microbiology*; D059013:Microbial Consortia / Q000502:physiology*; D010088:Oxidoreductases / Q000235:genetics; D010802:Phylogeny; D010944:Plants / Q000378:metabolism*; D058441:Rhizosphere
chemical_list: D000641:Ammonia; D010088:Oxidoreductases; C045582:ammonia monooxygenase