title: Simultaneous estimation of amlodipine besylate and nebivolol hydrochloride in tablet dosage forms by reverse phase-high-performance liquid chromatographic using ultraviolet detection.
abstract: BACKGROUND
  The present study aimed to develop and validate the simultaneous estimation of amlodipine and nebivolol in tablet dosage forms.
  
  
  METHODS
  An isocratic reversed phase high-performance liquid chromatographic (HPLC) method with ultraviolet detection at 268 nm has been developed for the determination of amlodipine besylate (ADB) and nebivolol hydrochloride in dosage formulation.
  
  
  RESULTS
  Good chromatographic separation was achieved by using a stainless steel analytical column, the Lichrospher ODS RP-18 column (250 × 4 mm), particle size 5 μm. The system was operated at ambient temperature (25 ± 2°C) using a mobile phase consisting of acetonitrile (ACN) and a phosphate buffer (pH 3.0), mixed in a ratio of 40 : 60 at a flow rate of 0.8 ml/minute. The slope, intercept, and correlation coefficient were found to be 8818.2, - 18159, and 0.9993 for amlodipine and 9048.7, 108595, and 0.9998 for nebivolol, respectively. The proposed method was validated for its specificity, linearity, accuracy, and precision.
  
  
  CONCLUSIONS
  The method was found to be suitable for the quality control of amlodipine besylate and nebivolol hydrochloride simultaneously in a bulk drug as well as in a formulation.
authors.affiliation: Department of Pharmaceutical Analysis, B.R. Nahata College of Pharmacy, Mhow-Neemuch Road, Mandsaur, (M.P)-458 001, India.
authors.forename: Deepak
authors.initials: D
authors.lastname: Sharma
authors.forename: Anurekha
authors.initials: A
authors.lastname: Jain
authors.forename: Alankar
authors.initials: A
authors.lastname: Shrivastava
keywords: Amlodipine besylate; RP-HPLC; isocratic separation; method validation; nebivolol hydrochloride