title: Cytotoxic effect and tissue penetration of phenol for adjuvant treatment of giant cell tumours.
abstract: Local adjuvant treatment of giant cell tumours (GCTs) of the bone with phenol has led to a significant reduction in recurrence rates. In the current study, the optimal phenol concentration and duration of intralesional exposure were evaluated. Specimens of GCTs were exposed to various concentrations of phenol solution (6, 60 and 80%) for either 1 or 3 min. Following embedding in glutaraldehyde, the tumour cell layers were examined by transmission electron microscopy. Destroyed cell organelles indicated the penetration depth as a sign of denaturation. Incubation of GCT specimens with 6% phenol solution for 3 min resulted in the most tissue damage and the deepest tissue penetration of ∼200μm. Incubation with 60 and 80% phenol solution reached a penetration depth of only ∼100μm. Phenol instillation may be used for the treatment of small scattered cellular debris following intralesional curettage; however, it is not suitable for treatment of remaining solid tumour tissue of GCT. The use of high phenol concentrations has no benefit and increases the risk of local or systemic intoxication.
authors.affiliation: Department of Orthopaedic Surgery, University Hospital Tuebingen, Tuebingen 72076;
authors.forename: Falk
authors.initials: F
authors.lastname: Mittag
authors.forename: Carmen
authors.initials: C
authors.lastname: Leichtle
authors.forename: Ina
authors.initials: I
authors.lastname: Kieckbusch
authors.forename: Hartwig
authors.initials: H
authors.lastname: Wolburg
authors.forename: Maximilian
authors.initials: M
authors.lastname: Rudert
authors.forename: Torsten
authors.initials: T
authors.lastname: Kluba
authors.forename: Ulf
authors.initials: U
authors.lastname: Leichtle
keywords: GCT; cytotoxic; denaturation; giant cell tumour; phenol; tissue penetration