title: Combining genome-wide methods to investigate the genetic complexity of courtship song variation in Drosophila melanogaster.
abstract: Little is currently known about the genetic complexity of quantitative behavioral variation, the types of genes involved, or their effects on intermediate phenotypes. Here, we conduct a genome-wide association study of Drosophila melanogaster courtship song variation using 168 sequenced inbred lines, and fail to find highly significant associations. However, by combining these data with results from a well-powered Evolve and Resequence (E&R) study on the same trait, we provide statistical evidence that some power to associate genotype and phenotype is available. Genes that are significant in both analyses are enriched for expression in the nervous system, and affect neural development and synaptic growth when perturbed. Quantitative complementation at one of these loci, Syntrophin-like 1, supports a hypothesis that variation at this locus affects variation in the inter-pulse interval of courtship song. These results suggest that experimental evolution may provide an approach for genome-scale replication in Drosophila.
authors.affiliation: Ecology, Evolution, and Marine Biology Department, University of California, Santa Barbara, USA. tturner@lifesci.ucsb.edu
authors.forename: Thomas L
authors.initials: TL
authors.lastname: Turner
authors.forename: Paige M
authors.initials: PM
authors.lastname: Miller
authors.forename: Veronica A
authors.initials: VA
authors.lastname: Cochrane
mesh_terms: D000818:Animals; D002135:Calcium-Binding Proteins / Q000235:genetics; D015815:Cell Adhesion Molecules / Q000235:genetics; D003380:Courtship; D029721:Drosophila Proteins / Q000235:genetics; D004331:Drosophila melanogaster / Q000235:genetics*; D019143:Evolution, Molecular; D005260:Female; D017344:Genes, Insect; D014644:Genetic Variation; D049750:Genome, Insect; D055106:Genome-Wide Association Study; D005838:Genotype; D008297:Male; D008565:Membrane Proteins / Q000235:genetics; D009124:Muscle Proteins / Q000235:genetics; D009419:Nerve Tissue Proteins / Q000235:genetics; D010641:Phenotype; D040641:Quantitative Trait Loci; D014828:Vocalization, Animal / Q000502:physiology
chemical_list: D002135:Calcium-Binding Proteins; D015815:Cell Adhesion Molecules; D029721:Drosophila Proteins; C491736:Dscam1 protein, Drosophila; D008565:Membrane Proteins; D009124:Muscle Proteins; D009419:Nerve Tissue Proteins; C091825:syntrophin alpha1
keywords: Drosophila; artificial selection; association studies; behavior; courtship; population genetics