title: Chimerism testing by quantitative PCR using Indel markers.
abstract: Engraftment monitoring is critical for patients after Hematopoietic Stem Cell Transplantation (HSCT). Complete donor chimerism is the goal; therefore, early detection of rejection and relapse is crucial for guiding the patient post HSCT treatment. Quantitative PCR for chimerism testing has been reported to be highly sensitive. In this chapter we discuss the quantitative PCR (qPCR) method using 34 Indel (Insertion and Deletion) genetic markers spread over 20 different chromosomes.
authors.affiliation: HLA laboratory, City of Hope, Duarte, CA, USA.
authors.forename: Ketevan
authors.initials: K
authors.lastname: Gendzekhadze
authors.forename: Laima
authors.initials: L
authors.lastname: Gaidulis
authors.forename: David
authors.initials: D
authors.lastname: Senitzer
mesh_terms: D005819:Genetic Markers; D006084:Graft Rejection / Q000235:genetics* / Q000276:immunology; D018380:Hematopoietic Stem Cell Transplantation; D006801:Humans; D054643:INDEL Mutation / Q000235:genetics; D008967:Molecular Biology / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods; D014019:Tissue Donors; D018183:Transplantation Chimera; D014184:Transplantation, Homologous
chemical_list: D005819:Genetic Markers