title: Ethanolic extract of Alternanthera sessilis (AS-1) inhibits IgE-mediated allergic response in RBL-2H3 cells.
abstract: The present study was designed to investigate the anti-allergic effects of ethanolic extract of Alternanthera sessilis (AS-1) in rat basophilic leukemia (RBL-2H3) cells. It significantly reduced the β-hexosaminidase release from anti-DNP-IgE sensitized RBL-2H3 cells. AS-1also inhibited the IgE antibody-induced increase in Interleukin-6 (IL-6), TNF-α, IL-13 and IL-4 production in these cells. The inhibitory effect of AS-1 on these cytokine was found to be nuclear factor-KB (NF-kB) dependent, as it attenuated the degradation of IKBa and nuclear translocation of NFkB. In addition, AS-1 significantly attenuated the DNP HAS-induced intracellular Ca(2+) release from these cells, which makes us speculate strongly that the decreased intracellular Ca(2+) is involved in the inhibitory effect of AS-1 on β-hexoaminidase release. Taken together, anti-allergic effects of AS-1 suggest possible therapeutic application of this extract in allergic diseases.
authors.affiliation: Pharmacology Division, Indian Institute of Integrative Medicine C.S.I.R., Jammu, India.
authors.forename: Sheikh
authors.initials: S
authors.lastname: Rayees
authors.forename: Ajay
authors.initials: A
authors.lastname: Kumar
authors.forename: Shafaq
authors.initials: S
authors.lastname: Rasool
authors.forename: Peerzada
authors.initials: P
authors.lastname: Kaiser
authors.forename: Naresh Kumar
authors.initials: NK
authors.lastname: Satti
authors.forename: Payare Lal
authors.initials: PL
authors.lastname: Sangwan
authors.forename: Surjeet
authors.initials: S
authors.lastname: Singh
authors.forename: Rakesh Kamal
authors.initials: RK
authors.lastname: Johri
authors.forename: Gurdarshan
authors.initials: G
authors.lastname: Singh
mesh_terms: D027703:Amaranthaceae; D000818:Animals; D018926:Anti-Allergic Agents / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism; D045744:Cell Line, Tumor; D016207:Cytokines / Q000378:metabolism; D004140:Dinitrophenols / Q000276:immunology; D000431:Ethanol / Q000737:chemistry; D006241:Haptens / Q000276:immunology; D007073:Immunoglobulin E / Q000276:immunology; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D012709:Serum Albumin / Q000276:immunology; D012997:Solvents / Q000737:chemistry; D001619:beta-N-Acetylhexosaminidases / Q000378:metabolism
chemical_list: D018926:Anti-Allergic Agents; D016207:Cytokines; D004140:Dinitrophenols; D006241:Haptens; D010936:Plant Extracts; D012709:Serum Albumin; D012997:Solvents; C058064:dinitrophenyl-human serum albumin conjugate; D007073:Immunoglobulin E; D000431:Ethanol; D007770:L-Lactate Dehydrogenase; D001619:beta-N-Acetylhexosaminidases; D002118:Calcium