title: Quantitative estimation of diacerein in bulk and in capsule formulation using hydrotropic solubilizing agents by UV-spectrophotometry and the first order derivative using the area under curve method.
abstract: OBJECTIVE
  This study was designed to develop and validate two simple, rapid, and economical UV-spectrophotometric and the first-order derivative methods using the area under curve method for estimation of diacerein in bulk and in capsule formulation.
  
  
  METHODS
  In this study, hydrotrophic solution of 8 M urea and 0.5 M potassium citrate were employed as the solubilizing agent to solubilize a poorly water-soluble drug, diacerein. In the UV-spectrophotometry method, two wavelengths 252.0 nm and 266.2 nm and in the first-order derivative spectrophotometric methods two wavelengths 259.4 nm and 274.2 nm in 8 M urea and two wavelengths 247.8 nm and 267.4 nm in the UV-spectrophotometry method and in the first-order derivative spectrophotometric methods two wavelengths 259.2 nm and 274.2 nm in 0.5 M potassium citrate were selected for determination of areas.
  
  
  RESULTS
  Hydrotrophic agents used did not interfere in spectrophotometric analysis of diacerein. Diacerein followed linearity in the concentration range of 2-12 μg/mL with a coefficient correlation of 0.999 for both methods.
  
  
  CONCLUSIONS
  The amount of drugs estimated by both proposed methods are in good accord with label claim. The % RSD value in recovery, precision, and ruggedness studies are found to be less than 2 indicate that the method is accurate, precise, and rugged.
authors.affiliation: H.R. Patel Institute of Pharmaceutical Education and Research, Shirpur, Dist: Dhule, Maharashtra, India.
authors.forename: Ramchandra
authors.initials: R
authors.lastname: Pandey
authors.forename: Pravin O
authors.initials: PO
authors.lastname: Patil
authors.forename: Manohar U
authors.initials: MU
authors.lastname: Patil
authors.forename: Prashant K
authors.initials: PK
authors.lastname: Deshmukh
authors.forename: Sanjay B
authors.initials: SB
authors.lastname: Bari
keywords: Diacerein; derivative spectroscopy; hydrotropic solubilization; potassium citrate; urea