title: Engagement of soluble resistance-related calcium binding protein (sorcin) with foot-and-mouth disease virus (FMDV) VP1 inhibits type I interferon response in cells.
abstract: Foot-and-mouth disease (FMD) is an acute, highly contagious animal disease caused by FMD virus (FMDV). Although FMDV-induced immunosuppression in host has been well established, the exact molecular mechanism for such induction is not very clear. We report here the identification of FMDV VP1 as an interferon-suppressor by interacting with soluble resistance-related calcium binding protein (sorcin). We found that VP1 suppressed tumor necrosis factor (TNF)-α or Sendai virus (SeV)-induced type I interferon response in HEK293T cells, and that this suppression could be completely abolished by knockdown of sorcin by shRNA. Furthermore, overexpression of sorcin inhibited type I interferon response. Conversely, TNF- or SeV-induced type I interferon response increased when sorcin knocked down, leading to inhibition of vesicular stomatitis virus (VSV) replication. Thus, VP1-induced suppression of type I interferon is mediated by interacting with sorcin, a protein that appears to regulate cell response to viral infections.
authors.affiliation: State Key Laboratory of Agrobiotechnology, China Agricultural University, Beijing 100193, China.
authors.forename: Xiaying
authors.initials: X
authors.lastname: Li
authors.forename: Jianchang
authors.initials: J
authors.lastname: Wang
authors.forename: Jue
authors.initials: J
authors.lastname: Liu
authors.forename: Zhonghua
authors.initials: Z
authors.lastname: Li
authors.forename: Yongqiang
authors.initials: Y
authors.lastname: Wang
authors.forename: Yanfei
authors.initials: Y
authors.lastname: Xue
authors.forename: Xiaoqi
authors.initials: X
authors.lastname: Li
authors.forename: Hong
authors.initials: H
authors.lastname: Cao
authors.forename: Shijun J
authors.initials: SJ
authors.lastname: Zheng
mesh_terms: D000818:Animals; D002135:Calcium-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D036022:Capsid Proteins / Q000235:genetics / Q000378:metabolism*; D005536:Foot-and-Mouth Disease / Q000235:genetics / Q000276:immunology / Q000378:metabolism* / Q000821:virology; D029544:Foot-and-Mouth Disease Virus / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007370:Interferon Type I / Q000096:biosynthesis*; D011485:Protein Binding; D034741:RNA, Small Interfering / Q000235:genetics; D013552:Swine; D013553:Swine Diseases / Q000235:genetics / Q000276:immunology / Q000378:metabolism* / Q000821:virology
chemical_list: D002135:Calcium-Binding Proteins; D036022:Capsid Proteins; D007370:Interferon Type I; D034741:RNA, Small Interfering; C503113:SRI protein, human; C442501:VP1 protein, Foot-and-mouth disease virus
keywords: FMDV; FMDV VP1; Immunosuppression; Interferon; SeV; Sendai virus; Sorcin; foot-and-mouth disease virus; soluble resistance-related calcium binding protein; sorcin