title: Polymethoxylated, C- and O-glycosyl flavonoids in tangelo (Citrus reticulata×Citrus paradisi) juice and their influence on antioxidant properties.
abstract: A separation/identification protocol based on RP-LC-DAD-ESI-MS-MS has been employed for the characterisation of the flavonoid fraction of the juice from tangelos (Citrus reticulata×Citrus paradisi) grown in Southern Italy. Eleven compounds were identified in a single chromatographic course. Of these, two C-glycosyl flavones (lucenin-2 and vicenin-2) and an O-triglycosyl flavanone (narirutin 4'-O-glucoside) were identified for the first time. Fruit juice antioxidant activity was evaluated on the basis of its ability to scavenge DPPH, O2(-), OH and ABTS(+) radicals, and to reduce iron (FRAP). Moreover, the influence of the identified polymethoxylated, C- and O-glycosyl flavonoids on the total antioxidant activity has been elucidated. We also checked the antimicrobial activity of a broad fraction, containing all the detected flavonoids obtained by preparative HPLC, in terms of MICs for Staphylococcus aureus.
authors.affiliation: Dipartimento di Scienze Chimiche, Università di Messina, Viale F. Stagno d'Alcontres 31, 98166 Messina, Italy. dbarreca@unime.it
authors.forename: Davide
authors.initials: D
authors.lastname: Barreca
authors.forename: Carlo
authors.initials: C
authors.lastname: Bisignano
authors.forename: Giovanna
authors.initials: G
authors.lastname: Ginestra
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Bisignano
authors.forename: Ersilia
authors.initials: E
authors.lastname: Bellocco
authors.forename: Ugo
authors.initials: U
authors.lastname: Leuzzi
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Gattuso
mesh_terms: D000975:Antioxidants / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D002957:Citrus / Q000737:chemistry; D005419:Flavonoids / Q000737:chemistry*; D005638:Fruit / Q000737:chemistry; D007558:Italy; D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D005419:Flavonoids; D010936:Plant Extracts