title: [Direct identification method for bacteria in positive blood culture bottles using MALDI biotyper].
abstract: We investigated the relevance of direct identification method for bacteria from blood culture bottles using MALDI Biotyper(Bruker Co.). One hundred fourteen bacterial strains obtained from blood culture bottles were analyzed using the pretreatment method recommended by Bruker Corporation. The identification rate with recommended pretreatment was 76.3% (87/114). Twenty seven samples that were not identified with the pretreatment method were further analyzed using our modified method. Of these, twelve were identified, improving the identification rate to 86.8% (99/114). These results suggest that our modified pretreatment combined with pretreatment method recommended by Bruker Corporation yields improved identification of bacteria from blood culture bottles using MALDI Biotyper system.
authors.affiliation: Department of Clinical Laboratory, Saga University Hospital, Saga 849-8501, Japan. ukinozo@cc.saga-u.ac.jp
authors.forename: Nozomi
authors.initials: N
authors.lastname: Uki
authors.forename: Megumi
authors.initials: M
authors.lastname: Oho
authors.forename: Zenzo
authors.initials: Z
authors.lastname: Nagasawa
authors.forename: Takanori
authors.initials: T
authors.lastname: Higashitani
authors.forename: Shoichiro
authors.initials: S
authors.lastname: Ohta
authors.forename: Eizaburo
authors.initials: E
authors.lastname: Sueoka
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Miyamoto
mesh_terms: D016470:Bacteremia / Q000382:microbiology*; D001419:Bacteria / Q000302:isolation & purification*; D001431:Bacteriological Techniques / Q000379:methods; D001770:Blood Bactericidal Activity; D006801:Humans; D012680:Sensitivity and Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*