title: Identification and characterization of the first Escherichia coli strain carrying NDM-1 gene in China.
abstract: New Delhi metallo-β-lactamase-1 (NDM-1), an acquired class B carbapenemase, is a significant clinical threat due to its extended hydrolysis of β-lactams including carbapenems. In this study, we identified the first confirmed clinical isolate of Escherichia coli BJ01 harboring bla(NDM-1) in China. The isolate is highly resistant to all tested antimicrobials except polymyxin. bla(NDM-1), bla(CTX-M-57), and bla TEM-1 were identified in the isolate. bla(NDM-1) was transferable to E. coli EC600 and DH5α in both plasmid conjugation experiments and plasmid transformation tests. BJ01 was identified as a new sequence type, ST224, by multilocus sequence typing. Analysis of genetic environment shows complex transposon-like structures surrounding the bla NDM-1 gene. Genetic analysis revealed that the region flanking bla(NDM-1) was very similar to previously identified NDM-positive Acinetobacter spp. isolated in China. The findings of this study raise attention to the emergence and spread of NDM-1-carrying Enterobacteriaceae in China.
authors.affiliation: Guang'anmen Hospital, China Academy of Chinese Medical Sciences, Beijing, PR China.
authors.forename: Zhiyuan
authors.initials: Z
authors.lastname: Liu
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.forename: Jie
authors.initials: J
authors.lastname: Wang
authors.forename: Jian
authors.initials: J
authors.lastname: Pan
authors.forename: Shipeng
authors.initials: S
authors.lastname: Sun
authors.forename: Yanhua
authors.initials: Y
authors.lastname: Yu
authors.forename: Bing
authors.initials: B
authors.lastname: Zhao
authors.forename: Yuzhi
authors.initials: Y
authors.lastname: Ma
authors.forename: Tingju
authors.initials: T
authors.lastname: Zhang
authors.forename: Jie
authors.initials: J
authors.lastname: Qi
authors.forename: Guijian
authors.initials: G
authors.lastname: Liu
authors.forename: Fengmin
authors.initials: F
authors.lastname: Lu
mesh_terms: D000368:Aged; D015139:Blotting, Southern; D015780:Carbapenems / Q000494:pharmacology*; D002681:China; D003227:Conjugation, Genetic; D004269:DNA, Bacterial / Q000235:genetics; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics*; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification*; D004927:Escherichia coli Infections / Q000188:drug therapy / Q000453:epidemiology / Q000382:microbiology*; D006801:Humans; D008826:Microbial Sensitivity Tests; D058885:Multilocus Sequence Typing; D010957:Plasmids / Q000235:genetics; D016133:Polymerase Chain Reaction; D014169:Transformation, Bacterial; D001618:beta-Lactamases / Q000235:genetics / Q000378:metabolism*
chemical_list: D015780:Carbapenems; D004269:DNA, Bacterial; D001618:beta-Lactamases; C552864:beta-lactamase NDM-1