title: Co-expression of antioxidant enzymes with expression of p53, DNA repair, and heat shock protein genes in the gamma ray-irradiated hermaphroditic fish Kryptolebias marmoratus larvae.
abstract: To investigate effects of gamma ray irradiation in the hermaphroditic fish, Kryptolebias marmoratus larvae, we checked expression of p53, DNA repair, and heat shock protein genes with several antioxidant enzyme activities by quantitative real-time RT-PCR and biochemical methods in response to different doses of gamma radiation. As a result, the level of gamma radiation-induced DNA damage was initiated after 4Gy of radiation, and biochemical and molecular damage became substantial from 8Gy. In particular, several DNA repair mechanism-related genes were significantly modulated in the 6Gy gamma radiation-exposed fish larvae, suggesting that upregulation of such DNA repair genes was closely associated with cell survival after gamma irradiation. The mRNA expression of p53 and most hsps was also significantly upregulated at high doses of gamma radiation related to cellular damage. This finding indicates that gamma radiation can induce oxidative stress with associated antioxidant enzyme activities, and linked to modulation of the expression of DNA repair-related genes as one of the defense mechanisms against radiation damage. This study provides a better understanding of the molecular mode of action of defense mechanisms upon gamma radiation in fish larvae.
authors.affiliation: Research Institute for Natural Sciences, Hanyang University, Seoul 133-791, South Korea.
authors.forename: Jae-Sung
authors.initials: JS
authors.lastname: Rhee
authors.forename: Bo-Mi
authors.initials: BM
authors.lastname: Kim
authors.forename: Ryeo-Ok
authors.initials: RO
authors.lastname: Kim
authors.forename: Jung Soo
authors.initials: JS
authors.lastname: Seo
authors.forename: Il-Chan
authors.initials: IC
authors.lastname: Kim
authors.forename: Young-Mi
authors.initials: YM
authors.lastname: Lee
authors.forename: Jae-Seong
authors.initials: JS
authors.lastname: Lee
mesh_terms: D000818:Animals; D003532:Cyprinodontiformes / Q000502:physiology*; D004260:DNA Repair / Q000235:genetics / Q000528:radiation effects; D004789:Enzyme Activation / Q000528:radiation effects; D005720:Gamma Rays; D005786:Gene Expression Regulation / Q000528:radiation effects*; D006360:Heat-Shock Proteins / Q000235:genetics*; D018384:Oxidative Stress / Q000528:radiation effects; D010088:Oxidoreductases / Q000235:genetics* / Q000378:metabolism; D016159:Tumor Suppressor Protein p53 / Q000235:genetics*
chemical_list: D006360:Heat-Shock Proteins; D016159:Tumor Suppressor Protein p53; D010088:Oxidoreductases
keywords: DNA repair; Gamma ray; Heat shock protein; Irradiation; Kryptolebias marmoratus; P53