title: Electrochemical sensor based on chlorohemin modified molecularly imprinted microgel for determination of 2,4-dichlorophenol.
abstract: A newly designed molecularly imprinted polymer (MIP) was synthesized and successfully utilized as a recognition element of an amperometric sensor for 2,4-dichlorophenol (2,4-DCP) detection. The MIP with a well-defined structure could imitate the dehalogenative function of the natural enzyme chloroperoxidase for 2,4-DCP. Imprinted sensor was fabricated in situ on a glassy carbon electrode surface by drop-coating the 2,4-DCP imprinted microgel suspension and chitosan/Nafion mixture. Under optimized conditions, the sensor showed a linear response in the range of 5.0-100 μmol L(-1) with a detection limit of 1.6 μmol L(-1). Additionally, the imprinted sensor demonstrated higher affinity to target 2,4-DCP over competitive chlorophenolic compounds than non-imprinted sensor. It also exhibited good stability and acceptable repeatability. The proposed sensor could be used for the determination of 2,4-DCP in water samples with the recoveries of 96.2-111.8%, showing a promising potential in practical application.
authors.affiliation: State Key Laboratory of Analytical Chemistry for Life Science, Department of Chemistry, Nanjing University, Nanjing 210093, PR China.
authors.forename: Jin
authors.initials: J
authors.lastname: Zhang
authors.forename: Jianping
authors.initials: J
authors.lastname: Lei
authors.forename: Huangxian
authors.initials: H
authors.lastname: Ju
authors.forename: Chaoying
authors.initials: C
authors.lastname: Wang
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D002733:Chlorophenols / Q000032:analysis / Q000737:chemistry*; D055664:Electrochemical Techniques / Q000379:methods* / Q000592:standards; D005782:Gels; D006427:Hemin / Q000032:analysis / Q000737:chemistry*; D054802:Molecular Imprinting / Q000379:methods* / Q000592:standards
chemical_list: D002733:Chlorophenols; D005782:Gels; D006427:Hemin; C004762:2,4-dichlorophenol