title: Identification and quantification of phytochemical composition and anti-inflammatory, cellular antioxidant, and radical scavenging activities of 12 Plantago species.
abstract: Twenty-eight seed samples of 12 Plantago species were investigated for their chemical compositions and anti-inflammatory, cellular antioxidant, and radical scavenging properties. A new UPLC-UV procedure was developed and applied to quantify acteoside and geniposidic acid, the characteristic constituents of the genus Plantago. The amounts of acteoside and geniposidic acid ranged from 0.07 to 15.96 mg/g and from 0.05 to 10.04 mg/g in the tested samples, respectively. Furthermore, 26 compounds were tentatively identified by UPLC/Q-TOF-MS analysis. The Plantago samples significantly differed in their phytochemical compositions. The extracts of Plantago seeds also showed inhibitory effects on LPS-induced IL-1β, IL-6, and COX-2 mRNA expression in RAW 264.7 mouse macrophage cells. Additionally, significant variations were observed among different samples on cellular antioxidant activities in HepG2 cells, as well as DPPH and hydroxyl radical scavenging capacities. The results from this study may be used to promote the use of the genus Plantago in improving human health.
authors.affiliation: Institute of Food and Nutraceutical Science, School of Agriculture and Biology, Shanghai Jiao Tong University , Shanghai 200240, China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Zhou
authors.forename: Weiying
authors.initials: W
authors.lastname: Lu
authors.forename: Yuge
authors.initials: Y
authors.lastname: Niu
authors.forename: Jie
authors.initials: J
authors.lastname: Liu
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Zhang
authors.forename: Boyan
authors.initials: B
authors.lastname: Gao
authors.forename: Casimir C
authors.initials: CC
authors.lastname: Akoh
authors.forename: Haiming
authors.initials: H
authors.lastname: Shi
authors.forename: Liangli Lucy
authors.initials: LL
authors.lastname: Yu
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry* / Q000494:pharmacology; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology; D002460:Cell Line; D016166:Free Radical Scavengers / Q000737:chemistry* / Q000494:pharmacology; D015870:Gene Expression / Q000187:drug effects; D056945:Hep G2 Cells; D006801:Humans; D053583:Interleukin-1beta / Q000235:genetics / Q000276:immunology; D015850:Interleukin-6 / Q000235:genetics / Q000276:immunology; D008264:Macrophages / Q000187:drug effects / Q000276:immunology; D051379:Mice; D010936:Plant Extracts / Q000737:chemistry* / Q000494:pharmacology; D010943:Plantago / Q000737:chemistry* / Q000145:classification; D012639:Seeds / Q000737:chemistry / Q000145:classification
chemical_list: D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D016166:Free Radical Scavengers; D053583:Interleukin-1beta; D015850:Interleukin-6; D010936:Plant Extracts