title: Cryphodera sinensis n. sp. (Nematoda: Heteroderidae), a non-cyst-forming parasitic nematode from the root of ramie Boehmeria nivea in China.
abstract: Cryphodera sinensis n. sp. is described from ramie (Boehmeria nivea) based on the morphology and molecular analyses of rRNA small subunit (SSU), D2D3 expansion domains of large subunit (LSU D2D3) and internal transcribed spacer (ITS). This new species is characterized by oval females with a distinct subcrystalline layer and pronounced and protruding vulval lip, distinctly concave vulva-anus profile and a vulva-anus distance of 29.5-35.8 μm. Males possess two annuli in the lip region, a stylet 27-32.5 μm in length with round knobs sloping slightly posteriorly, lateral fields with three lines, spicules 20-28 μm long and the presence of a short cloacal tube. Second-stage juveniles possess three lip annuli, a stylet 28-31 μm in length with well-developed knobs projected anteriorly and three lines along the lateral field. The pointed tail, 52-65 μm long, possesses a mucro-like tip and a hyaline region, 24.5-35 μm long. Large phasmids with a lens-like structure are located 2-6 annuli posterior to the anus. Phylogenetic analysis shows that the species has unique SSU, LSU D2D3 and ITS rRNA sequences. Phylogenetic relationships of the three rDNA sequences of C. sinensis n. sp. and other cystoid/cyst nematodes are analysed together with a comparison of other species within the genus Cryphodera.
authors.affiliation: Laboratory of Plant Nematology,South China Agricultural University,Guangzhou510642,China.
authors.forename: K
authors.initials: K
authors.lastname: Zhuo
authors.affiliation: Laboratory of Plant Nematology,South China Agricultural University,Guangzhou510642,China.
authors.forename: H H
authors.initials: HH
authors.lastname: Wang
authors.affiliation: Nematode Assay Section, Agronomic Division,North Carolina Department of Agriculture & Consumer Services,4300 Reedy Creek Road,Raleigh,NC27607,USA.
authors.forename: W
authors.initials: W
authors.lastname: Ye
authors.affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests,Institute of Plant Protection, Chinese Academy of Agricultural Sciences,Beijing100193,China.
authors.forename: D L
authors.initials: DL
authors.lastname: Peng
authors.affiliation: Laboratory of Plant Nematology,South China Agricultural University,Guangzhou510642,China.
authors.forename: J L
authors.initials: JL
authors.lastname: Liao
mesh_terms: D000818:Animals; D032402:Boehmeria / Q000469:parasitology*; D005260:Female; D008297:Male; D009348:Nematoda / Q000033:anatomy & histology* / Q000145:classification*; D010935:Plant Diseases / Q000469:parasitology*; D018517:Plant Roots / Q000469:parasitology*; D013045:Species Specificity