title: [Efficacy of Gram-Fungiflora Y double staining in diagnosing infectious keratitis].
abstract: OBJECTIVE
  To evaluate the efficacy of Gram-Fungiflora Y double staining for corneal scraping samples collected by impression in diagnosing infectious keratitis.
  
  
  METHODS
  Two hundred and thirteen eyes of 192 patients suspected of having infectious keratitis were retrospectively studied for the sensitivity and specificity of Gram-Fungiflora Y based on final diagnosis.
  
  
  RESULTS
  Of 165 infectious keratitis eyes, 107 had bacterial keratitis, 54 had fungal keratitis, and 15 had Acanthamoeba keratitis. Of these, 147 eyes were positive for one or other of the pathogens by the double staining method (overall sensitivity/specificity was 89.1% (95% confidence interval 83.1%-93.2%)/ 79.1% (64.6%-89.0%)). Sensitivity/specificity of the double staining for each of the pathogens was 88.8% 82.1% for bacterial keratitis, 81.4%/98.1% for fungal keratitis and 80.0%/97.0% for Acanthamoeba keratitis.
  
  
  CONCLUSIONS
  The double staining method for impression specimens was effective in diagnosing infectious keratitis with high sensitivity, and was especially useful for the diagnosis of fungal or Acanthamoeba keratitis.
authors.affiliation: Division of Ophthalmology and Visual Science, Faculty of Medicine, Tottori University, 36-1 Nishi-cho, Yonago-shi, Tottori-ken 683-8504, Japan. dm@grape.med.tottori-u.ac.jp
authors.forename: Dai
authors.initials: D
authors.lastname: Miyazaki
authors.forename: Hitomi
authors.initials: H
authors.lastname: Uotani
authors.forename: Ryu
authors.initials: R
authors.lastname: Uotani
authors.forename: Fumie
authors.initials: F
authors.lastname: Takenobu
authors.forename: Koudai
authors.initials: K
authors.lastname: Inata
authors.forename: Atsuko
authors.initials: A
authors.lastname: Miyake
authors.forename: Yoshitsugu
authors.initials: Y
authors.lastname: Inoue
mesh_terms: D015818:Eye Infections, Bacterial / Q000175:diagnosis / Q000382:microbiology*; D015821:Eye Infections, Fungal / Q000175:diagnosis / Q000382:microbiology*; D005840:Gentian Violet; D006801:Humans; D007634:Keratitis / Q000175:diagnosis* / Q000382:microbiology; D009930:Organic Chemicals / Q000032:analysis; D010619:Phenazines; D012680:Sensitivity and Specificity
chemical_list: C102655:Fungiflora Y; C035863:Gram's stain; D009930:Organic Chemicals; D010619:Phenazines; D005840:Gentian Violet