title: Application of phage display for ligand peptidomics to identify peptide ligands binding to AQP2-expressing membrane fractions.
abstract: In vitro phage display represents an emerging and innovative technology for the rapid isolation of high-affinity peptide ligands. Phage display technologies using phages comprising a vast library of peptides have become fundamental to the isolation of high-affinity binding ligands for diagnostic and therapeutic applications, e.g., ligand proteomics, discovery of novel protein-protein interactions, antibody engineering, targeted delivery of therapeutic agents, and development of imaging probes. This chapter describes the procedures for phage display selection of peptide ligands that selectively bind to aquaporin-2-expressing membrane fractions of rat kidney.
authors.affiliation: Department of Biochemistry and Cell Biology, School of Medicine, Kyungpook National University, Taegu, South Korea.
authors.forename: Byung-Heon
authors.initials: BH
authors.lastname: Lee
authors.forename: Tae-Hwan
authors.initials: TH
authors.lastname: Kwon
mesh_terms: D000818:Animals; D051399:Aquaporin 2 / Q000378:metabolism*; D002462:Cell Membrane / Q000737:chemistry / Q000378:metabolism*; D002478:Cells, Cultured; D019793:Fluorescein / Q000032:analysis; D007425:Intracellular Membranes / Q000737:chemistry / Q000378:metabolism; D007668:Kidney / Q000737:chemistry / Q000166:cytology / Q000378:metabolism; D008024:Ligands; D019151:Peptide Library; D010455:Peptides / Q000032:analysis* / Q000378:metabolism*; D011485:Protein Binding; D025941:Protein Interaction Mapping / Q000379:methods*; D040901:Proteomics / Q000379:methods; D051381:Rats; D020539:Sequence Analysis, Protein / Q000379:methods; D013194:Staining and Labeling / Q000379:methods
chemical_list: D051399:Aquaporin 2; D008024:Ligands; D019151:Peptide Library; D010455:Peptides; D019793:Fluorescein