title: Next generation Advanced Laser Fluorometry (ALF) for characterization of natural aquatic environments: new instruments.
abstract: The new optical design allows single- or multi-wavelength excitation of laser-stimulated emission (LSE), provides optimized LSE optical collection for spectral and temporal analyses, and incorporates swappable modules for flow-through and small-volume sample measurements. The basic instrument configuration uses 510 nm laser excitation for assessments of chlorophyll-a, phycobiliprotein pigments, variable fluorescence (F(v)/F(m)) and chromophoric dissolved organic matter (CDOM) in CDOM-rich waters. The three-laser instrument configuration (375, 405, and 510 nm excitation) provides additional Fv/Fm measurements with 405 nm excitation, CDOM assessments in a broad concentration range, and potential for spectral discrimination between oil and CDOM fluorescence. The new measurement protocols, analytical algorithms and examples of laboratory and field measurements are discussed.
authors.affiliation: Lamont Doherty Earth Observatory of Columbia University, 61 Route 9W, Palisades, NY 10964, USA. chekaluk@ldeo.columbia.edu
authors.forename: Alexander
authors.initials: A
authors.lastname: Chekalyuk
authors.forename: Mark
authors.initials: M
authors.lastname: Hafez
mesh_terms: D002734:Chlorophyll / Q000032:analysis*; D004784:Environmental Monitoring / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005470:Fluorometry / Q000295:instrumentation*; D005618:Fresh Water / Q000032:analysis*; D007834:Lasers; D013057:Spectrum Analysis / Q000295:instrumentation*
chemical_list: D002734:Chlorophyll