title: Determination of antimycin-A in water by liquid chromatographic/mass spectrometry: single-laboratory validation.
abstract: An LC/MS method was developed and validated for the quantitative determination and confirmation of antimycin-A (ANT-A) in water from lakes or streams. Three different water sample volumes (25, 50, and 250 mL) were evaluated. ANT-A was stabilized in the field by immediately extracting it from water into anhydrous acetone using SPE. The stabilized concentrated samples were then transported to a laboratory and analyzed by LC/MS using negative electrospray ionization. The method was determined to have adequate accuracy (78 to 113% recovery), precision (0.77 to 7.5% RSD with samples > or = 500 ng/L and 4.8 to 17% RSD with samples < or = 100 ng/L), linearity, and robustness over an LOQ range from 8 to 51 600 ng/L.
authors.affiliation: U.S. Geological Survey, Upper Midwest Environmental Sciences Center, 2630 Fanta Reed Rd, La Crosse, WI 54603, USA. jbernardy@usgs.gov
authors.forename: Jeffry A
authors.initials: JA
authors.lastname: Bernardy
authors.forename: Terrance D
authors.initials: TD
authors.lastname: Hubert
authors.forename: Jacob M
authors.initials: JM
authors.lastname: Ogorek
authors.forename: Larry J
authors.initials: LJ
authors.lastname: Schmidt
mesh_terms: D000968:Antimycin A / Q000737:chemistry*; D002853:Chromatography, Liquid / Q000379:methods*; D013058:Mass Spectrometry / Q000379:methods*; D015394:Molecular Structure; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014867:Water / Q000737:chemistry*; D014874:Water Pollutants, Chemical / Q000737:chemistry*
chemical_list: D014874:Water Pollutants, Chemical; D014867:Water; D000968:Antimycin A