title: Effects of micro environmental factors on natural killer activity (NK) of beta thalassemia major patients.
abstract: The physiological mechanisms of decreased NK activity of β-Thalassemia major (BTM) patients are unknown. To assess in vitro effects of mononuclear cells and their cytokine secretion on NK activity, we compared activator receptor levels and cytotoxic activity of purified NK cells and NK cells in mononuclear cells (MNC) pools. We collected cell supernatant from unincubated and incubated MNC with K562 cells and measured their secreted cytokines levels. CD16 was lower on the surface of NK cells in MNC pools from BTM patients compared to healthy volunteers. This inhibition does not appear when NK cells were purified. NKp30 levels in NK cells decreased both as purified cells and as part of a pool of MNC in BTM patients. After incubation of MNC pools with K562 target cells, we found that supernatant levels of IL10, TGFβ1 and IL15 cytokines were also significantly higher in BTM patients compared to healthy volunteers.
authors.affiliation: Istanbul University, Istanbul Faculty of Medicine, Physiology Department, Istanbul, Turkey. belkisatasever.arslan@uskudar.edu.tr
authors.forename: Belkis
authors.initials: B
authors.lastname: Atasever Arslan
authors.forename: Serap
authors.initials: S
authors.lastname: Erdem-Kuruca
authors.forename: Zeynep
authors.initials: Z
authors.lastname: Karakas
authors.forename: Batu
authors.initials: B
authors.lastname: Erman
authors.forename: Arzu
authors.initials: A
authors.lastname: Ergen
mesh_terms: D000293:Adolescent; D000328:Adult; D060833:Cellular Microenvironment / Q000187:drug effects / Q000276:immunology*; D002648:Child; D017077:Culture Media, Conditioned / Q000378:metabolism / Q000494:pharmacology; D016207:Cytokines / Q000276:immunology / Q000378:metabolism / Q000494:pharmacology; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D016753:Interleukin-10 / Q000276:immunology / Q000378:metabolism / Q000494:pharmacology; D019409:Interleukin-15 / Q000276:immunology / Q000378:metabolism / Q000494:pharmacology; D020014:K562 Cells; D007694:Killer Cells, Natural / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism; D008297:Male; D055628:Natural Cytotoxicity Triggering Receptor 3 / Q000276:immunology / Q000378:metabolism; D017452:Receptors, IgG / Q000276:immunology / Q000378:metabolism; D053773:Transforming Growth Factor beta1 / Q000276:immunology / Q000378:metabolism / Q000494:pharmacology; D055815:Young Adult; D017086:beta-Thalassemia / Q000276:immunology* / Q000473:pathology
chemical_list: D017077:Culture Media, Conditioned; D016207:Cytokines; D019409:Interleukin-15; C431710:NCR3 protein, human; D055628:Natural Cytotoxicity Triggering Receptor 3; D017452:Receptors, IgG; D053773:Transforming Growth Factor beta1; D016753:Interleukin-10
keywords: CD16; Cytokine; IL10; IL15; NK activity; NKp30; TGFβ1