title: Comparative metabolism study of β-lapachone in mouse, rat, dog, monkey, and human liver microsomes using liquid chromatography-tandem mass spectrometry.
abstract: β-Lapachone (3,4-dihydro-2,2-dimethyl-2H-naphthol[1,2-b]pyran-5,6-dione) is a natural compound extracted from the bark of the lapacho tree (Tabebuia avellanedae) and is undergoing phase II clinical trials as an antitumor drug candidate. The present study characterized in vitro metabolites of β-lapachone in mouse, rat, dog, monkey and human liver microsomes. β-Lapachone (10 μM) was incubated with mouse, rat, dog, monkey, and human liver microsomes in the presence of NADPH. The reaction mixtures were analyzed by LC/MS and the metabolites were identified based on their elemental composition and product ion spectra. A total of 6 metabolites (M1-M6) were detected in liver microsomes with a slight difference between species. M1 and M6 were identified as a decarbonated metabolite and a carboxylated metabolite, respectively; M2, M3, and M4 were identified as monohydroxylated metabolites; and M5 was identified as an O-methylated metabolite. M5, an O-methylated metabolite was found in rat and human liver microsomes, which is thought to be formed from a catechol intermediate by MB-COMT-mediated methylation and reported here for the first time.
authors.affiliation: College of Pharmacy, Research Institute of Pharmaceutical Sciences, Kyungpook National University, Daegu 702-701, Republic of Korea.
authors.forename: Sangkyu
authors.initials: S
authors.lastname: Lee
authors.forename: In Sook
authors.initials: IS
authors.lastname: Kim
authors.forename: Tae Hwan
authors.initials: TH
authors.lastname: Kwak
authors.forename: Hye Hyun
authors.initials: HH
authors.lastname: Yoo
mesh_terms: D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D004285:Dogs; D000882:Haplorhini; D006801:Humans; D051379:Mice; D008862:Microsomes, Liver / Q000378:metabolism*; D009249:NADP / Q000737:chemistry; D009285:Naphthoquinones / Q000737:chemistry* / Q000378:metabolism*; D051381:Rats; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D009285:Naphthoquinones; D009249:NADP; C014638:beta-lapachone