title: Profiling and quantifying differential gene transcription provide insights into ganoderic acid biosynthesis in Ganoderma lucidum in response to methyl jasmonate.
abstract: Ganoderma lucidum is a mushroom with traditional medicinal properties that has been widely used in China and other countries in Eastern Asia. Ganoderic acids (GA) produced by G. lucidum exhibit important pharmacological activities. Previous studies have demonstrated that methyl jasmonate (MeJA) is a potent inducer of GA biosynthesis and the expression of genes involved in the GA biosynthesis pathway in G. lucidum. To further explore the mechanism of GA biosynthesis, cDNA-Amplified Fragment Length Polymorphism (cDNA-AFLP) was used to identify genes that are differentially expressed in response to MeJA. Using 64 primer combinations, over 3910 transcriptionally derived fragments (TDFs) were obtained. Reliable sequence data were obtained for 390 of 458 selected TDFs. Ninety of these TDFs were annotated with known functions through BLASTX searching the GenBank database, and 12 annotated TDFs were assigned into secondary metabolic pathways by searching the KEGGPATHWAY database. Twenty-five TDFs were selected for qRT-PCR analysis to confirm the expression patterns observed with cDNA-AFLP. The qRT-PCR results were consistent with the altered patterns of gene expression revealed by the cDNA-AFLP technique. Additionally, the transcript levels of 10 genes were measured at the mycelium, primordia, and fruiting body developmental stages of G. lucidum. The greatest expression levels were reached during primordia for all of the genes except cytochrome b2 reached its highest expression level in the mycelium stage. This study not only identifies new candidate genes involved in the regulation of GA biosynthesis but also provides further insight into MeJA-induced gene expression and secondary metabolic response in G. lucidum.
authors.affiliation: Microbiology Department, College of Life Sciences, Nanjing Agricultural University, Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, Nanjing, Jiangsu, P.R. China.
authors.forename: Ang
authors.initials: A
authors.lastname: Ren
authors.forename: Meng-Jiao
authors.initials: MJ
authors.lastname: Li
authors.forename: Liang
authors.initials: L
authors.lastname: Shi
authors.forename: Da-Shuai
authors.initials: DS
authors.lastname: Mu
authors.forename: Ai-Liang
authors.initials: AL
authors.lastname: Jiang
authors.forename: Qin
authors.initials: Q
authors.lastname: Han
authors.forename: Ming-Wen
authors.initials: MW
authors.lastname: Zhao
mesh_terms: D000085:Acetates / Q000494:pharmacology*; D054458:Amplified Fragment Length Polymorphism Analysis; D001483:Base Sequence; D003517:Cyclopentanes / Q000494:pharmacology*; D018076:DNA, Complementary / Q000235:genetics; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D018506:Gene Expression Regulation, Plant / Q000187:drug effects*; D017343:Genes, Plant / Q000235:genetics; D053858:Metabolic Networks and Pathways / Q000187:drug effects / Q000235:genetics; D054883:Oxylipins / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020940:Reishi / Q000187:drug effects* / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014158:Transcription, Genetic / Q000187:drug effects*; D014315:Triterpenes / Q000378:metabolism*
chemical_list: D000085:Acetates; D003517:Cyclopentanes; D018076:DNA, Complementary; D054883:Oxylipins; D012333:RNA, Messenger; D014315:Triterpenes; C556862:ganoderic acid; C072239:methyl jasmonate