title: The Conyza triloba extracts with high chlorophyll content and free radical scavenging activity had anticancer activity in cell lines.
abstract: The discovery of anticancer agents paradigm has been shifted to natural resources to overcome the toxicity of many synthetic agents at early clinical stages. In the present study, the antimutagenic, anticancer, phytochemistry, and free radical scavenging activities of five extracts of Conyza triloba were investigated. Extracts II (water : methanol), III (methylene chloride), and IV (methylene chloride : methanol) had the highest chlorophyll content and the highest superoxide scavenging, and metal chelating activities comparable to that of trolox. They also showed DPPH(•) scavenging activities better than that of α -tocopherol. Virtually all extracts exerted a strong (>40% reduction) antimutagenic activity against sodium azide and benzopyrene. Extracts II, III, and IV showed a remarkable growth inhibition profile with GI50 of 0.07-0.87 μg for Hepa1c1c7 and H4IIE1, A549, HT29, and PC3 cell lines and totally abated the growth of all cell lines, except for the breast cells, at 0.3-7.0 μg. The present study found a strong correlation between the chlorophyll content of Conyza extracts and their DDPH scavenging, metal chelating, and in vitro cytotoxic and cytostatic activities most probably through triggering apoptosis. This study could offer a platform for future studies and help selecting the vital features that identify the extract with potential anticancer activities.
authors.affiliation: Department of Biological Sciences, Faculty of Science, King Faisal University, Al-Hofuf, Al-Ahsa 31982, Saudi Arabia. welhalawany@kfu.edu.sa
authors.forename: Wael M
authors.initials: WM
authors.lastname: El-Sayed
authors.forename: Warda A
authors.initials: WA
authors.lastname: Hussin
authors.forename: Ahmed A
authors.initials: AA
authors.lastname: Mahmoud
authors.forename: Mohamed A
authors.initials: MA
authors.lastname: AlFredan
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000378:metabolism; D001564:Benzo(a)pyrene; D002338:Carotenoids / Q000378:metabolism; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002614:Chelating Agents / Q000494:pharmacology; D002734:Chlorophyll / Q000378:metabolism*; D035401:Conyza / Q000737:chemistry*; D005419:Flavonoids / Q000378:metabolism; D016166:Free Radical Scavengers / Q000494:pharmacology*; D006801:Humans; D000077276:Lycopene; D050296:Microbial Viability / Q000187:drug effects; D016296:Mutagenesis / Q000187:drug effects; D059645:Mutation Rate; D010936:Plant Extracts / Q000494:pharmacology*; D012486:Salmonella typhimurium / Q000187:drug effects; D019810:Sodium Azide; D019207:beta Carotene / Q000378:metabolism
chemical_list: D000970:Antineoplastic Agents; D000975:Antioxidants; D002614:Chelating Agents; D005419:Flavonoids; D016166:Free Radical Scavengers; D010936:Plant Extracts; D019207:beta Carotene; D002734:Chlorophyll; D001564:Benzo(a)pyrene; D002338:Carotenoids; D019810:Sodium Azide; D000077276:Lycopene