title: Computational analysis of four human adenovirus type 4 genomes reveals molecular evolution through two interspecies recombination events.
abstract: Computational analysis of human adenovirus type 4 (HAdV-E4), a pathogen that is the only HAdV member of species E, provides insights into its zoonotic origin and molecular adaptation. Its genome encodes a domain of the major capsid protein, hexon, from HAdV-B16 recombined into the genome chassis of a simian adenovirus. Genomes of two recent field strains provide a clue to its adaptation to the new host: recombination of a NF-I binding site motif, which is required for efficient viral replication, from another HAdV genome. This motif is absent in the chimpanzee adenoviruses and the HAdV-E4 prototype, but is conserved amongst other HAdVs. This is the first report of an interspecies recombination event for HAdVs, and the first documentation of a lateral partial gene transfer from a chimpanzee AdV. The potential for such recombination events are important when considering chimpanzee adenoviruses as candidate gene delivery vectors for human patients.
authors.affiliation: Chemistry Department, American University, Washington, D.C. 20016, USA; Bioinformatics and Computational Biology Program, School of Systems Biology, George Mason University, Manassas, VA 20110, USA.
authors.forename: Shoaleh
authors.initials: S
authors.lastname: Dehghan
authors.forename: Jason
authors.initials: J
authors.lastname: Seto
authors.forename: Elizabeth B
authors.initials: EB
authors.lastname: Liu
authors.forename: Michael P
authors.initials: MP
authors.lastname: Walsh
authors.forename: David W
authors.initials: DW
authors.lastname: Dyer
authors.forename: James
authors.initials: J
authors.lastname: Chodosh
authors.forename: Donald
authors.initials: D
authors.lastname: Seto
mesh_terms: D000258:Adenovirus Infections, Human / Q000821:virology; D000260:Adenoviruses, Human / Q000235:genetics*; D000261:Adenoviruses, Simian / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D019295:Computational Biology / Q000379:methods*; D019143:Evolution, Molecular; D022761:Gene Transfer, Horizontal; D016679:Genome, Viral / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D002679:Pan troglodytes / Q000821:virology; D010802:Phylogeny; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D014764:Viral Proteins / Q000235:genetics; D015047:Zoonoses
chemical_list: D014764:Viral Proteins
keywords: Adenovirus; Molecular evolution; Recombination; Zoonosis