title: Substance P enhances HIV-1 infection in human fetal brain cell cultures expressing full-length neurokinin-1 receptor.
abstract: The associations between the neurokinin-1 receptor (NK-1R), substance P (SP), and HIV-1 were investigated in neurosphere-derived cultures of microglial-depleted human fetal brain cells (HFBC). Full-length NK-1R was identified in HFBC cultures. SP treatment of the HFBC increased intracellular calcium mobilization and decreased electrical impedance, both of which were blocked by the NK-1R antagonist aprepitant. SP treatment of HIV-1-infected HFBC upregulated HIV-1 expression. These data show that human neural cells grown from neurospheres express functional full length NK-1R that is responsive to SP, and that SP enhanced HIV-1 infection in HBFC.
authors.affiliation: Research Institute, Children's Hospital of Philadelphia, Suite 1208, 3615 Civic Center Boulevard, Philadelphia, PA 19104, USA. schwartzl1@email.chop.edu
authors.forename: Lynnae
authors.initials: L
authors.lastname: Schwartz
authors.forename: Sergei V
authors.initials: SV
authors.lastname: Spitsin
authors.forename: John
authors.initials: J
authors.lastname: Meshki
authors.forename: Florin
authors.initials: F
authors.lastname: Tuluc
authors.forename: Steven D
authors.initials: SD
authors.lastname: Douglas
authors.forename: John H
authors.initials: JH
authors.lastname: Wolfe
mesh_terms: D000077608:Aprepitant; D001921:Brain / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism / Q000821:virology; D002118:Calcium / Q000378:metabolism; D017097:Electric Impedance; D005333:Fetus; D015870:Gene Expression; D015497:HIV-1 / Q000187:drug effects* / Q000502:physiology; D054884:Host-Pathogen Interactions; D006801:Humans; D017136:Ion Transport / Q000187:drug effects; D009025:Morpholines / Q000494:pharmacology; D064729:Neurokinin-1 Receptor Antagonists / Q000494:pharmacology; D009474:Neurons / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism / Q000821:virology; D061251:Primary Cell Culture; D018040:Receptors, Neurokinin-1 / Q000235:genetics* / Q000378:metabolism; D013373:Substance P / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D014775:Virus Activation / Q000187:drug effects
chemical_list: D009025:Morpholines; D064729:Neurokinin-1 Receptor Antagonists; D018040:Receptors, Neurokinin-1; D000077608:Aprepitant; D013373:Substance P; D002118:Calcium