title: Estimation of sertraline by chromatographic (HPLC-UV273 nm) technique under hydrolytic stress conditions.
abstract: OBJECTIVE
  In this paper, simple, specific and accurate RP-HPLC method was developed in order to study decomposition of sertraline (SRT) under the hydrolytic stress conditions (acid, neutral, alkaline and oxidative).
  
  
  METHODS
  The best separation of SRT and its degradation products were achieved on reverse phase LiChoCART with Purospher (RP-18e) column. The mobile phase was composed of methanol/water (75:25, v/v). The detection wavelength was 273 nm. The method was validated and response was found to be linear in the drug concentration range of 10-200 μg ml-1 with correlation coefficient of 0.998.
  
  
  RESULTS
  The RSD values for intra- and inter-day precision were < 0.65 and < 0.72%, respectively. Employing RP-HPLC method, degradation products were detected in the exposed samples.
  
  
  CONCLUSIONS
  IT WAS FOUND THAT THE SUSCEPTIBILITY OF SRT TO HYDROLYTIC DECOMPOSITION INCREASED IN FOLLOWING MANNER: Neutral condition < alkaline condition < acid condition < oxidative condition.
authors.affiliation: Faculty of Pharmacy, Integral University, Kursi Road, Lucknow - 226 026, India.
authors.forename: Md Akhlaquer
authors.initials: MA
authors.lastname: Rahman
authors.forename: Zeenat
authors.initials: Z
authors.lastname: Iqbal
authors.forename: Mohd Aamir
authors.initials: MA
authors.lastname: Mirza
authors.forename: Arshad
authors.initials: A
authors.lastname: Hussain
keywords: Antidepressant; high-performance liquid chromatography; hydrolysis; limit of detection; limit of quantitation; sertraline; stability