title: Acute promyelocytic leukemia mutated to radioresistance suppressed monocyte lineage differentiation by phorbol 12-myristate 13-acetate.
abstract: Induction of myeloid differentiation in radioresistant HL60 cells (Res-HL60) was examined to clarify the developmental mechanism of radioresistant leukemia. Compared to wild-type HL60 cells (Wt-HL60), Res-HL60 were smaller and strongly expressed CD38. Under all-trans retinoic acid (ATRA) stimulation, Res-HL60 continued to proliferate slowly and with similar level of CD11b expression to Wt-HL60. Phorbol 12-myristate 13-acetate (PMA) strongly suppressed proliferation of Res-HL60, downregulated CD14, and affected mRNA expression. These results suggested that the specific myeloid differentiation of Res-HL60 suppressed monocyte lineage by ATRA and PMA occurred through regulation of mRNA expression.
authors.affiliation: Department of Radiological Life Sciences, Hirosaki University Graduate School of Health Sciences, 66-1 Hon-cho, Hirosaki, Aomori, Japan.
authors.forename: Satoru
authors.initials: S
authors.lastname: Monzen
authors.forename: Kodai
authors.initials: K
authors.lastname: Takimura
authors.forename: Ikuo
authors.initials: I
authors.lastname: Kashiwakura
authors.forename: Yoichiro
authors.initials: Y
authors.lastname: Hosokawa
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism; D002273:Carcinogens / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects* / Q000528:radiation effects*; D019070:Cell Lineage; D005434:Flow Cytometry; D020869:Gene Expression Profiling; D018922:HL-60 Cells; D006801:Humans; D015473:Leukemia, Promyelocytic, Acute / Q000188:drug therapy / Q000473:pathology* / Q000532:radiotherapy; D009000:Monocytes / Q000166:cytology / Q000187:drug effects / Q000528:radiation effects; D009154:Mutation / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis; D011836:Radiation Tolerance / Q000187:drug effects*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*; D014212:Tretinoin / Q000494:pharmacology; D014407:Tumor Cells, Cultured; D014965:X-Rays
chemical_list: D000970:Antineoplastic Agents; D014408:Biomarkers, Tumor; D002273:Carcinogens; D014212:Tretinoin; D013755:Tetradecanoylphorbol Acetate
keywords: APL; CD11b; CD14; CD38; Myeloid differentiation; Radiation resistant