title: The in vitro and in vivo effects of yerba mate (Ilex paraguariensis) extract on adipogenesis.
abstract: The aim of this study was to evaluate the effects of yerba mate extract and its principal bioactive compounds on adipogenesis. The anti-adipogenic effects of yerba mate, chlorogenic acid, quercetin and rutin were evaluated in 3T3-L1 cells using a PCR array. The results obtained in vitro were validated in vivo in a high-fat diet-induced model of obesity. The in vitro and in vivo results demonstrated that yerba mate extract down-regulated the expression of genes that regulate adipogenesis, such as Creb-1and C/EBPα, and the extract up-regulated the expression of genes related to the inhibition of adipogenesis, including Dlk1, Gata2, Gata3, Klf2, Lrp5, Pparγ2, Sfrp1, Tcf7l2, Wnt10b, and Wnt3a. In summary, it was demonstrated that yerba mate and its bioactive compounds regulate the expression of genes related to in vitro adipogenesis. Furthermore, yerba mate might regulate adipogenesis through the Wnt pathway.
authors.affiliation: Unidade Integrada de Farmacologia e Gastroenterologia, Universidade São Francisco, Bragança Paulista, SP, Brazil.
authors.forename: Demétrius Paiva
authors.initials: DP
authors.lastname: Arçari
authors.forename: Juliana Carvalho
authors.initials: JC
authors.lastname: Santos
authors.forename: Alessandra
authors.initials: A
authors.lastname: Gambero
authors.forename: Marcelo Lima
authors.initials: ML
authors.lastname: Ribeiro
mesh_terms: D041721:3T3-L1 Cells; D050156:Adipogenesis / Q000187:drug effects*; D000818:Animals; D050882:CREB-Binding Protein / Q000235:genetics / Q000378:metabolism; D002726:Chlorogenic Acid / Q000008:administration & dosage; D050989:GATA2 Transcription Factor / Q000235:genetics / Q000378:metabolism; D050990:GATA3 Transcription Factor / Q000235:genetics / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D030019:Ilex paraguariensis / Q000737:chemistry*; D008297:Male; D051379:Mice; D009765:Obesity / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism / Q000503:physiopathology; D010936:Plant Extracts / Q000008:administration & dosage*; D011794:Quercetin / Q000008:administration & dosage; D012431:Rutin / Q000008:administration & dosage
chemical_list: D050989:GATA2 Transcription Factor; D050990:GATA3 Transcription Factor; C494712:Gata2 protein, mouse; C494748:Gata3 protein, mouse; D010936:Plant Extracts; D002726:Chlorogenic Acid; D012431:Rutin; D011794:Quercetin; D050882:CREB-Binding Protein; C494364:Crebbp protein, mouse