title: Prolonged sitting and markers of cardiometabolic disease risk in children and youth: a randomized crossover study.
abstract: OBJECTIVE
  Recent evidence suggests that short bouts of uninterrupted sedentary behavior reduce insulin sensitivity and glucose tolerance while increasing triglyceride levels in both healthy and overweight/obese adults. To date no study has examined the acute impact of uninterrupted sitting in children and youth. The objective of the present study was to determine whether 8 h of uninterrupted sitting increases markers of cardiometabolic disease risk in healthy children and youth, in comparison to 8 h of sitting interrupted by light intensity walk breaks or structured physical activity.
  
  
  METHODS
  11 healthy males and 8 healthy females between the ages of 10 and 14 years experienced 3 conditions in random order: (1) 8 h of uninterrupted sitting (Sedentary); (2) 8 h of sitting interrupted with a 2-min light-intensity walk break every 20 min (Breaks); and (3) 8 h of sitting interrupted with a 2-min light-intensity walk break every 20 min as well as 2×20 min of moderate-intensity physical activity (Breaks+Physical Activity). Insulin, glucose, triglyceride, HDL and LDL cholesterol area under the curve were calculated for each condition.
  
  
  RESULTS
  We observed no significant differences in the area under the curve for any marker of cardiometabolic disease risk across the 3 study conditions (all p>0.09).
  
  
  CONCLUSIONS
  These results suggest that in comparison to interrupted sitting or structured physical activity, a single bout of 8 h of uninterrupted sitting does not result in measurable changes in circulating levels of insulin, glucose, or lipids in healthy children and youth.
authors.affiliation: Healthy Active Living and Obesity Research Group, Children's Hospital of Eastern Ontario Research Institute, 401 Smyth Road, Ottawa, ON, Canada; School of Human Kinetics, University of Ottawa, 125 University Avenue, Ottawa, ON, Canada. Electronic address: saunders.travis@gmail.com.
authors.forename: Travis J
authors.initials: TJ
authors.lastname: Saunders
authors.forename: Jean-Philippe
authors.initials: JP
authors.lastname: Chaput
authors.forename: Gary S
authors.initials: GS
authors.lastname: Goldfield
authors.forename: Rachel C
authors.initials: RC
authors.lastname: Colley
authors.forename: Glen P
authors.initials: GP
authors.lastname: Kenny
authors.forename: Eric
authors.initials: E
authors.lastname: Doucet
authors.forename: Mark S
authors.initials: MS
authors.lastname: Tremblay
mesh_terms: D001786:Blood Glucose / Q000378:metabolism; D002318:Cardiovascular Diseases / Q000097:blood* / Q000209:etiology / Q000378:metabolism*; D002648:Child; D008076:Cholesterol, HDL / Q000097:blood; D008078:Cholesterol, LDL / Q000097:blood; D018592:Cross-Over Studies; D015444:Exercise / Q000502:physiology; D005260:Female; D006801:Humans; D007328:Insulin / Q000097:blood; D008297:Male; D011187:Posture / Q000502:physiology*; D012306:Risk; D014280:Triglycerides / Q000097:blood; D016138:Walking / Q000502:physiology
chemical_list: D001786:Blood Glucose; D008076:Cholesterol, HDL; D008078:Cholesterol, LDL; D007328:Insulin; D014280:Triglycerides
keywords: BMI; Body mass index; Glucose tolerance; HDL; High density lipoprotein; Incremental area under the curve; Insulin sensitivity; LDL; Low density lipoprotein; Pediatric population; REE; Resting Energy Expenditure; Sedentary behavior; iAUC