title: Effects of a marine serine protease inhibitor on viability and morphology of Trypanosoma cruzi, the agent of Chagas disease.
abstract: It has been reported that serine peptidase activities of Trypanosoma cruzi play crucial roles in parasite dissemination and host cell invasion and therefore their inhibition could affect the progress of Chagas disease. The present study investigates the interference of the Stichodactyla helianthus Kunitz-type serine protease inhibitor (ShPI-I), a 55-amino acid peptide, in T. cruzi serine peptidase activities, parasite viability, and parasite morphology. The effect of this peptide was also studied in Leishmania amazonensis promastigotes and it was proved to be a powerful inhibitor of serine proteases activities and the parasite viability. The ultrastructural alterations caused by ShPI-I included vesiculation of the flagellar pocket membrane and the appearance of a cytoplasmic vesicle that resembles an autophagic vacuole. ShPI-I, which showed itself to be an important T. cruzi serine peptidase inhibitor, reduced the parasite viability, in a dose and time dependent manner. The maximum effect of peptide on T. cruzi viability was observed when ShPI-I at 1×10(-5)M was incubated for 24 and 48h which killed completely both metacyclic trypomastigote and epimastigote forms. At 1×10(-6)M ShPI-I, in the same periods of time, reduced parasite viability about 91-95% respectively. Ultrastructural analysis demonstrated the formation of concentric membranar structures especially in the cytosol, involving organelles and small vesicles. Profiles of endoplasmic reticulum were also detected, surrounding cytosolic vesicles that resembled autophagic vacuoles. These results suggest that serine peptidases are important in T. cruzi physiology since the inhibition of their activity killed parasites in vitro as well as inducing important morphological alterations. Protease inhibitors thus appear to have a potential role as anti-trypanosomatidal agents.
authors.affiliation: Laboratório de Interação de Tripanosomatídeos e Vetores, Departamento de Bioquímica, Instituto de Biologia Roberto Alcântara Gomes, Universidade do Estado do Rio de Janeiro, UERJ, Rio de Janeiro, RJ, Brazil.
authors.forename: Natália Pereira
authors.initials: NP
authors.lastname: de Almeida Nogueira
authors.forename: José Andrés
authors.initials: JA
authors.lastname: Morgado-Díaz
authors.forename: Rubem Figueiredo Sadok
authors.initials: RF
authors.lastname: Menna-Barreto
authors.forename: Marcia Cristina
authors.initials: MC
authors.lastname: Paes
authors.forename: Raquel Elisa
authors.initials: RE
authors.lastname: da Silva-López
mesh_terms: D000818:Animals; D000981:Antiprotozoal Agents / Q000302:isolation & purification / Q000494:pharmacology*; D059001:Aquatic Organisms / Q000737:chemistry; D001688:Biological Products / Q000302:isolation & purification / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects*; D014355:Chagas Disease / Q000469:parasitology; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007891:Leishmania / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D008854:Microscopy, Electron; D015388:Organelles / Q000648:ultrastructure; D012615:Sea Anemones / Q000737:chemistry*; D015843:Serpins / Q000302:isolation & purification / Q000494:pharmacology*; D014349:Trypanosoma cruzi / Q000166:cytology / Q000187:drug effects* / Q000502:physiology
chemical_list: D000981:Antiprotozoal Agents; D001688:Biological Products; D015843:Serpins
keywords: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide; BHI; BPTI; Cell viability; EM; Electron microscopy; FBS; IC(50); Kunitz-type serine protease inhibitor from Sea anemone Stichodactyla helianthus; L-BAPNA; MTT; N(α)-Benzoyl-l-arginine 4-nitroanilide hydrochloride; PBS; Peptidase inhibition; ShPI-I; T. cruzi prolyl oligopeptidase with 80-kDa; TAU; Tc80; Trypanosoma cruzi; bovine pancreatic trypsin inhibitor; brain heart infusion; electron microscopy; fetal bovine serum; half maximal inhibitory concentration; phosphate buffer saline; triatomine artificial urine