title: Platelet-rich plasma promotes the proliferation of human muscle derived progenitor cells and maintains their stemness.
abstract: Human muscle-derived progenitor cells (hMDPCs) offer great promise for muscle cell-based regenerative medicine; however, prolonged ex-vivo expansion using animal sera is necessary to acquire sufficient cells for transplantation. Due to the risks associated with the use of animal sera, the development of a strategy for the ex vivo expansion of hMDPCs is required. The purpose of this study was to investigate the efficacy of using platelet-rich plasma (PRP) for the ex-vivo expansion of hMDPCs. Pre-plated MDPCs, myoendothelial cells, and pericytes are three populations of hMDPCs that we isolated by the modified pre-plate technique and Fluorescence Activated Cell Sorting (FACS), respectively. Pooled allogeneic human PRP was obtained from a local blood bank, and the effect that thrombin-activated PRP-releasate supplemented media had on the ex-vivo expansion of the hMDPCs was tested against FBS supplemented media, both in vitro and in vivo. PRP significantly enhanced short and long-term cell proliferation, with or without FBS supplementation. Antibody-neutralization of PDGF significantly blocked the mitogenic/proliferative effects that PRP had on the hMDPCs. A more stable and sustained expression of markers associated with stemness, and a decreased expression of lineage specific markers was observed in the PRP-expanded cells when compared with the FBS-expanded cells. The in vitro osteogenic, chondrogenic, and myogenic differentiation capacities of the hMDPCs were not altered when expanded in media supplemented with PRP. All populations of hMDPCs that were expanded in PRP supplemented media retained their ability to regenerate myofibers in vivo. Our data demonstrated that PRP promoted the proliferation and maintained the multi-differentiation capacities of the hMDPCs during ex-vivo expansion by maintaining the cells in an undifferentiated state. Moreover, PDGF appears to be a key contributing factor to the beneficial effect that PRP has on the proliferation of hMDPCs.
authors.affiliation: Department of Orthopedic Surgery, Stem Cell Research Center, University of Pittsburgh, Pittsburgh, Pennsylvania, United States of America.
authors.forename: Hongshuai
authors.initials: H
authors.lastname: Li
authors.forename: Arvydas
authors.initials: A
authors.lastname: Usas
authors.forename: Minakshi
authors.initials: M
authors.lastname: Poddar
authors.forename: Chien-Wen
authors.initials: CW
authors.lastname: Chen
authors.forename: Seth
authors.initials: S
authors.lastname: Thompson
authors.forename: Bahar
authors.initials: B
authors.lastname: Ahani
authors.forename: James
authors.initials: J
authors.lastname: Cummins
authors.forename: Mitra
authors.initials: M
authors.lastname: Lavasani
authors.forename: Johnny
authors.initials: J
authors.lastname: Huard
mesh_terms: D000368:Aged; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D005434:Flow Cytometry; D006801:Humans; D008297:Male; D051379:Mice; D016513:Mice, SCID; D008875:Middle Aged; D008934:Mitogens / Q000494:pharmacology; D032342:Muscle Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D024510:Muscle Development / Q000187:drug effects; D010982:Platelet-Derived Growth Factor / Q000378:metabolism; D053657:Platelet-Rich Plasma / Q000378:metabolism*; D013234:Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism
chemical_list: D015415:Biomarkers; D008934:Mitogens; D010982:Platelet-Derived Growth Factor