title: Translating next generation sequencing to practice: opportunities and necessary steps.
abstract: Next-generation sequencing (NGS) approaches for measuring RNA and DNA benefit from greatly increased sensitivity, dynamic range and detection of novel transcripts. These technologies are rapidly becoming the standard for molecular assays and represent huge potential value to the practice of oncology. However, many challenges exist in the transition of these technologies from research application to clinical practice. This review discusses the value of NGS in detecting mutations, copy number changes and RNA quantification and their applications in oncology, the challenges for adoption and the relevant steps that are needed for translating this potential to routine practice.
authors.affiliation: Philips Research North America, Briarcliff Manor, NY 10510, USA. sitharthk@philips.com
authors.forename: Sitharthan
authors.initials: S
authors.lastname: Kamalakaran
authors.forename: Vinay
authors.initials: V
authors.lastname: Varadan
authors.forename: Angel
authors.initials: A
authors.lastname: Janevski
authors.forename: Nilanjana
authors.initials: N
authors.lastname: Banerjee
authors.forename: David
authors.initials: D
authors.lastname: Tuck
authors.forename: W Richard
authors.initials: WR
authors.lastname: McCombie
authors.forename: Nevenka
authors.initials: N
authors.lastname: Dimitrova
authors.forename: Lyndsay N
authors.initials: LN
authors.lastname: Harris
mesh_terms: D056915:DNA Copy Number Variations / Q000235:genetics; D023281:Genomics / Q000379:methods; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D006801:Humans; D008297:Male; D009154:Mutation; D009369:Neoplasms / Q000235:genetics; D017422:Sequence Analysis, DNA
keywords: Genomics; Next generation sequencing; Oncology; Personalized medicine