title: Identification of honokiol metabolites in rats by the method of stable isotope cluster technique and ultra-high performance liquid chromatography/quadrupole-time-of-flight mass spectrometry.
abstract: Honokiol, a natural molecule isolated from Magnolia officinalis Rehd. et Wils., is widely known as an antitumor agent. In present work, an analysis of in vivo biotransformation and metabolites of honokiol has been performed by a combined method based on stable isotope cluster technique with honokiol-[(13)C6]-labeled and ultra-high performance liquid chromatography/quadrupole-time-of-flight-mass spectrometry (UHPLC/Q-TOF-MS). The metabolites could be easily identified by the determination of a chromatographically co-eluted pair of isotopomers (MS doublet peaks) with similar peak intensities and mass difference corresponding to that between isotope-labeled and non-isotope-labeled honokiol. A total of eighteen metabolites were detected and tentatively identified, fourteen of which were reported for the first time. The results indicated that the main metabolic pathways of honokiol in rats were hydroxylation, methylation, sulfation and glucuronidation. This study provided the first essential information on biotransformation and metabolites of honokiol in rats, which was very useful for further pharmacological and clinical studies of honokiol as a potent drug candidate.
authors.affiliation: State Key Laboratory of Biotherapy, West China Hospital, West China Medical School, Sichuan University, Chengdu 610041, China; College of Chemical Engineering, Sichuan University, Chengdu 610065, China.
authors.forename: Huijun
authors.initials: H
authors.lastname: Lai
authors.forename: Minghai
authors.initials: M
authors.lastname: Tang
authors.forename: Juan
authors.initials: J
authors.lastname: Liu
authors.forename: Yinfeng
authors.initials: Y
authors.lastname: Dong
authors.forename: Neng
authors.initials: N
authors.lastname: Qiu
authors.forename: Shucai
authors.initials: S
authors.lastname: Li
authors.forename: Liang
authors.initials: L
authors.lastname: Ma
authors.forename: Jianhong
authors.initials: J
authors.lastname: Yang
authors.forename: Hang
authors.initials: H
authors.lastname: Song
authors.forename: Yongkui
authors.initials: Y
authors.lastname: Zhang
authors.forename: Aihua
authors.initials: A
authors.lastname: Peng
authors.forename: Lijuan
authors.initials: L
authors.lastname: Chen
mesh_terms: D000818:Animals; D001713:Biphenyl Compounds / Q000097:blood / Q000737:chemistry / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D020719:Glucuronides / Q000097:blood / Q000737:chemistry / Q000378:metabolism; D005971:Glutamates / Q000097:blood / Q000737:chemistry / Q000378:metabolism; D007553:Isotope Labeling; D017705:Lignans / Q000097:blood / Q000737:chemistry / Q000378:metabolism*; D008297:Male; D013058:Mass Spectrometry / Q000379:methods*; D053858:Metabolic Networks and Pathways; D051381:Rats; D017207:Rats, Sprague-Dawley; D000498:Allyl Compounds; D010636:Phenols
chemical_list: D001713:Biphenyl Compounds; D020719:Glucuronides; D005971:Glutamates; D017705:Lignans; C005499:honokiol; D000498:Allyl Compounds; D010636:Phenols