title: Multifunctional fluorescent probe for sequential detections of glutathione and caspase-3 in vitro and in cells.
abstract: Herein, we report a new "On-On" strategy based on the assembly and disassembly of fluorescein isothiocyanate nanoparticles (FITC-NPs) for sequential detections of glutathione (GSH) and caspase-3 (Casp3) with a multifunctional fluorescent probe 1. Theoretical investigations revealed the underlying mechanism that satisfactorily explained experimental results of such consecutive enhancements of fluorescence. Using this probe, we also successfully imaged the Casp3 activity in apoptotic cells.
authors.affiliation: CAS Key Laboratory of Soft Matter Chemistry, Department of Chemistry, University of Science and Technology of China, 96 Jinzhai Road, Hefei, Anhui 230026, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Huang
authors.forename: Xijun
authors.initials: X
authors.lastname: Wang
authors.forename: Dingli
authors.initials: D
authors.lastname: Wang
authors.forename: Fang
authors.initials: F
authors.lastname: Liu
authors.forename: Bin
authors.initials: B
authors.lastname: Mei
authors.forename: Anming
authors.initials: A
authors.lastname: Tang
authors.forename: Jun
authors.initials: J
authors.lastname: Jiang
authors.forename: Gaolin
authors.initials: G
authors.lastname: Liang
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D053148:Caspase 3 / Q000032:analysis*; D045744:Cell Line, Tumor; D016650:Fluorescein-5-isothiocyanate / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry*; D005978:Glutathione / Q000032:analysis*; D006801:Humans; D053758:Nanoparticles / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000379:methods
chemical_list: D005456:Fluorescent Dyes; D053148:Caspase 3; D005978:Glutathione; D016650:Fluorescein-5-isothiocyanate