title: [Postconditioning can reduce long-term lung injury after lower limb ischemia-reperfusion].
abstract: BACKGROUND
  Operation on the infrarenal aorta could cause ischemic-reperfusion (IR) injury in local tissues and remote organs (e.g. the lung).
  
  
  OBJECTIVE
  Our aim was to reduce long-term lung damage, after lower limb IR with postconditioning.
  
  
  METHODS
  Male Wistar rats underwent 180 minutes of bilateral lower limb ischemia. Animals were divided into three groups: Sham-operated, IR, Postconditioned (PostC) and further to two subgroups according to reperfusion time: 24 h and 72 h. Serum free radical and IL-6 levels, histological changes, Wet/Dry (W/D) ratio, tissue myeloperoxidase (MPO) activity and Hsp72 levels were investigated.
  
  
  RESULTS
  Postconditioning can reduce histological changes in the lung. Free radical levels are significantly lower in PostC groups than in IR groups (42.9 ± 8.0 vs. 6.4 ± 3.4; 27.3 ± 4.4 vs. 8.3 ± 4.0 RLU%; p < 0.05). IL-6 level (238.4 ± 31.1 vs. 209.1 ± 18.8; 190.0 ± 8.8 vs. 187.0 ± 14.9 pg/ml) and Hsp72 expression did not show any significant difference. Compared to the IR group, lung MPO activity did not change in the PostC groups. W/D ratio in PostC groups is significantly lower at all measured time-points (68% vs. 65%; 72% vs. 68%; p < 0.05).
  
  
  CONCLUSIONS
  Postconditioning may reduce long-term damages of the lung after lower limb ischemic-reperfusion injury.
authors.affiliation: Semmelweis Egyetem, I. sz. Sebészeti Klinika, 1082 Budapest, Üllői út. 78.
authors.forename: Dávid
authors.initials: D
authors.lastname: Garbaisz
authors.forename: Zsolt
authors.initials: Z
authors.lastname: Turóczi
authors.forename: András
authors.initials: A
authors.lastname: Fülöp
authors.forename: Olivér
authors.initials: O
authors.lastname: Rosero
authors.forename: Péter
authors.initials: P
authors.lastname: Arányi
authors.forename: Péter
authors.initials: P
authors.lastname: Ónody
authors.forename: Gábor
authors.initials: G
authors.lastname: Lotz
authors.forename: Zoltán
authors.initials: Z
authors.lastname: Rakonczay
authors.forename: Zsolt
authors.initials: Z
authors.lastname: Balla
authors.forename: László
authors.initials: L
authors.lastname: Harsányi
authors.forename: Attila
authors.initials: A
authors.lastname: Szijártó
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000097:blood; D005609:Free Radicals / Q000378:metabolism; D050884:HSP72 Heat-Shock Proteins / Q000097:blood; D015850:Interleukin-6 / Q000097:blood; D057775:Ischemic Postconditioning; D035002:Lower Extremity / Q000098:blood supply*; D055370:Lung Injury / Q000209:etiology / Q000473:pathology / Q000517:prevention & control*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000517:prevention & control*; D013997:Time Factors
chemical_list: D015415:Biomarkers; D005609:Free Radicals; D050884:HSP72 Heat-Shock Proteins; D015850:Interleukin-6