title: Bacterial artificial chromosomes (BACs)-on-Beads™ as a diagnostic platform for the rapid aneuploidy screening of products of conception.
abstract: The aim of the present study was to evaluate the use of KaryoLite™ bacterial artificial chromosomes (BACs)‑on‑Beads™ (BoBs) technology for the rapid screening of products of conception (POC). Validation and prospective studies were carried out on 85 and 95 patient samples, respectively. Validation studies had previously been analyzed using routine culture and G-banded karyotyping. BoBs resulted in an abnormality detection frequency of 27%, with a failure rate of <3%. The time required for processing was significantly lower compared with that of tissue culture. In conclusion, BoBs technology decreased the failure rate, while increasing the analytical sensitivity compared with G-banded karyotype analysis alone. Additionally, significant cost savings may be achieved with regard to the time of processing and analysis of specimens.
authors.affiliation: Diagnostic Genetics, LabPlus, Auckland City Hospital, Auckland 1148, New Zealand.
authors.forename: Karen L
authors.initials: KL
authors.lastname: Sheath
authors.forename: Lisa
authors.initials: L
authors.lastname: Duffy
authors.forename: Philip
authors.initials: P
authors.lastname: Asquith
authors.forename: Donald R
authors.initials: DR
authors.lastname: Love
authors.forename: Alice M
authors.initials: AM
authors.lastname: George
mesh_terms: D000782:Aneuploidy; D002869:Chromosome Aberrations; D002871:Chromosome Banding; D025063:Chromosome Disorders / Q000175:diagnosis; D022202:Chromosomes, Artificial, Bacterial / Q000235:genetics*; D005260:Female; D005820:Genetic Testing / Q000379:methods*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D008297:Male; D011446:Prospective Studies; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity