title: Optofluidic realization and retaining of cell-cell contact using an abrupt tapered optical fibre.
abstract: Studies reveal that there exists much interaction and communication between bacterial cells, with parts of these social behaviors depending on cell-cell contacts. The cell-cell contact has proved to be crucial for determining various biochemical processes. However, for cell culture with relatively low cell concentration, it is difficult to precisely control and retain the contact of a small group of cells. Particularly, the retaining of cell-cell contact is difficult when flows occur in the medium. Here, we report an optofluidic method for realization and retaining of Escherichia coli cell-cell contact in a microfluidic channel using an abrupt tapered optical fibre. The contact process is based on launching a 980-nm wavelength laser into the fibre, E. coli cells were trapped onto the fibre tip one after another, retaining cell-cell contact and forming a highly organized cell chain. The formed chains further show the ability as bio-optical waveguides.
authors.affiliation: State Key Laboratory of Optoelectronic Materials and Technologies, School of Physics and Engineering, Sun Yat-Sen University, Guangzhou 510275, China.
authors.forename: Hongbao
authors.initials: H
authors.lastname: Xin
authors.forename: Yao
authors.initials: Y
authors.lastname: Zhang
authors.forename: Hongxiang
authors.initials: H
authors.lastname: Lei
authors.forename: Yayi
authors.initials: Y
authors.lastname: Li
authors.forename: Huixian
authors.initials: H
authors.lastname: Zhang
authors.forename: Baojun
authors.initials: B
authors.lastname: Li
mesh_terms: D002450:Cell Communication; D004926:Escherichia coli / Q000166:cytology; D044085:Microfluidics / Q000295:instrumentation; D055100:Optical Fibers