title: Expression of catalase in Lactobacillus fermentum and evaluation of its anti-oxidative properties in a dextran sodium sulfate induced mouse colitis model.
abstract: Lactic acid bacteria are generally sensitive to hydrogen peroxide (H₂O₂). Lactobacillus plantarum ATCC14431 is one of the few lactic acid bacteria able to degrade H₂O₂ through the action of a manganese-dependent catalase (containing the katA gene). However, it is not a natural inhabitant of the intestinal tract and its bio-efficacy and survival in the gastrointestinal tract have never been tested. In this study, we successfully expressed the katA gene from L. plantarum ATCC14431 in L. fermentum I5007 and the recombinant L. fermentum exhibited almost 20-fold higher catalase activity than the empty vector control. The anti-oxidative properties of this catalase-producing L. fermentum were evaluated using a dextran sodium sulphate (DSS) induced colitis mice model. Compared with the control, mice receiving DSS alone had increased diarrhea and mucosa histological scores (P < 0.05), as well as lipid peroxidation (P < 0.05), myeloperoxidase (P < 0.05), and active NF-κB in colonic tissue (P < 0.05). Similar to vitamin E, treatment with recombinant L. fermentum mitigate these effects accompanied by a improvement in mucosa histological scores in the proximal colon (P < 0.05) and decreased lipid peroxidation (P < 0.05), myeloperoxidase (P < 0.05) and active NF-κB in colonic tissue (P < 0.05). In conclusion, the expression of catalase in L. fermentum increased its ability to survive when exposed to aerated environment in vitro and conferred the anti-oxidative and anti-inflammatory effects in the DSS induced colitis model.
authors.affiliation: State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing, 100193, China.
authors.forename: Jiang
authors.initials: J
authors.lastname: Zhang
authors.forename: Hong
authors.initials: H
authors.lastname: Liu
authors.forename: Qingwei
authors.initials: Q
authors.lastname: Wang
authors.forename: Chengli
authors.initials: C
authors.lastname: Hou
authors.forename: Philip
authors.initials: P
authors.lastname: Thacker
authors.forename: Shiyan
authors.initials: S
authors.lastname: Qiao
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D002374:Catalase / Q000235:genetics* / Q000378:metabolism*; D003092:Colitis / Q000139:chemically induced / Q000378:metabolism / Q000382:microbiology / Q000628:therapy*; D003106:Colon / Q000378:metabolism / Q000382:microbiology; D016264:Dextran Sulfate; D004195:Disease Models, Animal; D005260:Female; D052197:Limosilactobacillus fermentum / Q000201:enzymology* / Q000235:genetics; D048191:Lactiplantibacillus plantarum / Q000235:genetics*; D015227:Lipid Peroxidation; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D050296:Microbial Viability; D019936:Probiotics; D011994:Recombinant Proteins / Q000378:metabolism; D014169:Transformation, Bacterial
chemical_list: D000975:Antioxidants; D001426:Bacterial Proteins; D011994:Recombinant Proteins; D016264:Dextran Sulfate; D002374:Catalase