title: Dual effects of chitosan decoration on the liposomal membrane physicochemical properties as affected by chitosan concentration and molecular conformation.
abstract: This study was devoted to a further understanding of the dependence of liposomal membrane properties on chitosan conformation and proved the dual effects of chitosan. The concentration dependence of chitosan conformation in aqueous solution was illustrated by surface tension and fluorescence probe techniques. Fluorescence and Raman spectra were subsequently employed to investigate the dynamic and structural changes of the liposomal membrane resulting from chitosan decoration. Results showed that the unfolded and crimped chains of chitosan flatly adsorbed onto the membrane surface via electrostatic attraction and favored liposome stability. Furthermore, the adsorption of crimped chains seemed stronger due to the embedding of their hydrophobic moieties. However, the presence of chitosan coils induced the increase in membrane fluidity, the intrachain disorder in lipid molecules, and the gauche conformation change of choline group. Dynamic light scattering and lipid oxidation measurements demonstrated that this perturbation was correlated with the permeation of coils into the lipid bilayer.
authors.affiliation: State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Tan
authors.forename: Jin
authors.initials: J
authors.lastname: Xue
authors.forename: Karangwa
authors.initials: K
authors.lastname: Eric
authors.forename: Biao
authors.initials: B
authors.lastname: Feng
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Zhang
authors.forename: Shuqin
authors.initials: S
authors.lastname: Xia
mesh_terms: D000327:Adsorption; D002236:Carbohydrate Conformation; D055598:Chemical Phenomena; D048271:Chitosan / Q000737:chemistry* / Q000494:pharmacology; D008027:Light; D008051:Lipid Bilayers / Q000737:chemistry; D015227:Lipid Peroxidation; D008081:Liposomes / Q000737:chemistry*; D008560:Membrane Fluidity; D012542:Scattering, Radiation; D012996:Solutions; D013050:Spectrometry, Fluorescence; D013059:Spectrum Analysis, Raman; D055672:Static Electricity; D014867:Water
chemical_list: D008051:Lipid Bilayers; D008081:Liposomes; D012996:Solutions; D014867:Water; D048271:Chitosan