title: Collection, isolation and characterization of the stem cells of umbilical cord blood.
abstract: The umbilical cord blood (UCB) is a rich source of hematopoietic and mesenchymal stem cells and it can be used for cellular therapy. This article presents data achieved from 50 cord blood samples (46 in utero, four ex utero). The nucleated cells were isolated from the cord blood using a dextran solution. The quantity of stem cells having the immunophenotype CD34+, CD45+ and CD90+ was determined, using flow cytometry. The nucleated cells were cultivated in a DMEM (Dulbecco's Modified Eagle Medium) and biochemical and cytogenetic tests was later performed.
authors.affiliation: Laboratory of Tissue Engineering and Cell Cultures, Department of Obstetrics and Gynecology, "Nicolae Testemitanu" State Medical and Pharmaceutical University, Kishinev, Republic of Moldova. revencut@yahoo.com
authors.forename: Tatiana
authors.initials: T
authors.lastname: Revencu
authors.forename: Victoria
authors.initials: V
authors.lastname: Trifan
authors.forename: Ludmila
authors.initials: L
authors.lastname: Nacu
authors.forename: Tatiana
authors.initials: T
authors.lastname: Gutium
authors.forename: L
authors.initials: L
authors.lastname: Globa
authors.forename: A G M
authors.initials: AG
authors.lastname: Motoc
authors.forename: V
authors.initials: V
authors.lastname: Nacu
mesh_terms: D000293:Adolescent; D000328:Adult; D002469:Cell Separation; D002478:Cells, Cultured; D020732:Cytogenetic Analysis; D005260:Female; D005312:Fetal Blood / Q000166:cytology*; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000378:metabolism; D006801:Humans; D016130:Immunophenotyping / Q000379:methods; D007231:Infant, Newborn; D008297:Male; D011247:Pregnancy; D055815:Young Adult