title: Methods for the Pasteurella glycosaminoglycan synthases: enzymes that polymerize hyaluronan, chondroitin, or heparosan chains.
abstract: Multiple glycosyltransferases (GTases) that produce glycosaminoglycans (GAGs) have been identified; -several distinct putative architectures and catalytic abilities have been noted from microbes and vertebrates. Here the preparation and use of a class of enzymes (Class II) from the gram-negative bacterium, Pasteurella multocida, with utility in chemoenzymatic synthesis of GAGs is described. The analyses of sugar products include thin layer chromatography (TLC), matrix-assisted laser desorption ionization mass spectroscopy (MALDI-ToF MS), and polyacrylamide gel electrophoresis (PAGE). The related Chapter 18 is focused on larger molecular weight GAGs analyses as well as the Class I membrane streptococcal and mammalian HA synthases.
authors.affiliation: Department of Biochemistry and Molecular Biology, The Oklahoma Center for Medical Glycobiology, The University of Oklahoma Health Sciences Center, Oklahoma City, OK, USA.
authors.forename: Paul L
authors.initials: PL
authors.lastname: DeAngelis
mesh_terms: D002807:Chondroitin / Q000032:analysis / Q000378:metabolism; D002855:Chromatography, Thin Layer / Q000379:methods; D004187:Disaccharides / Q000032:analysis / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D006025:Glycosaminoglycans / Q000032:analysis / Q000378:metabolism*; D016695:Glycosyltransferases / Q000378:metabolism*; D006820:Hyaluronic Acid / Q000032:analysis / Q000378:metabolism; D016979:Pasteurella multocida / Q000201:enzymology* / Q000378:metabolism; D058105:Polymerization; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods
chemical_list: D004187:Disaccharides; D006025:Glycosaminoglycans; C560363:heparosan; D006820:Hyaluronic Acid; D002807:Chondroitin; D016695:Glycosyltransferases