title: A method for determination of UDP-GlcNAc: GlcNAcβ1-6(GlcNAcβ1-2)Manα1-R [GlcNAc to Man] β1-4N-acetylglucosaminyltransferase VI activity.
abstract: To characterize and purify glycosyltransferases, it is essential to establish a simple and sensitive assay method. Here, we describe a method for determination of the activity of GnT VI (UDP-GlcNAc: GlcNAcβ1-6(GlcNAcβ1-2)Manα1-R [GlcNAc to Man] β1-4N-acetylglucosaminyltransferase VI) using a fluorescently labeled oligosaccharide.
authors.affiliation: Department of Health Chemistry, Graduate School of Pharmaceutical Sciences, University of Tokyo, Tokyo, Japan.
authors.forename: Tomohiko
authors.initials: T
authors.lastname: Taguchi
authors.forename: Naoyuki
authors.initials: N
authors.lastname: Taniguchi
mesh_terms: D000818:Animals; D002240:Carbohydrate Sequence; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D057075:Enzyme Assays / Q000379:methods*; D005456:Fluorescent Dyes / Q000032:analysis / Q000378:metabolism; D006801:Humans; D008969:Molecular Sequence Data; D017351:N-Acetylglucosaminyltransferases / Q000302:isolation & purification / Q000378:metabolism*; D009844:Oligosaccharides / Q000032:analysis / Q000378:metabolism
chemical_list: D005456:Fluorescent Dyes; D009844:Oligosaccharides; D017351:N-Acetylglucosaminyltransferases; C417184:UDP-N-acetylglucosamine N-acetyl-D-glucosaminyl-1-6-(N-acetylglucosaminyl-1-2)mannopyranosyl-1-R(N-acetylglucosamine to mannose)-1,4N-acetylglucosaminyltransferase VI