title: Isolation and characterization of alkane degrading bacteria from petroleum reservoir waste water in Iran (Kerman and Tehran provenances).
abstract: Petroleum products spill and leakage have become two major environmental challenges in Iran. Sampling was performed in the petroleum reservoir waste water of Tehran and Kerman Provinces of Iran. Alkane degrading bacteria were isolated by enrichment in a Bushnel-Hass medium, with hexadecane as sole source of carbon and energy. The isolated strains were identified by amplification of 16S rDNA gene and sequencing. Specific primers were used for identification of alkane hydroxylase gene. Fifteen alkane degrading bacteria were isolated and 8 strains were selected as powerful degradative bacteria. These 8 strains relate to Rhodococcus jostii, Stenotrophomonas maltophilia, Achromobacter piechaudii, Tsukamurella tyrosinosolvens, Pseudomonas fluorescens, Rhodococcus erythropolis, Stenotrophomonas maltophilia, Pseudomonas aeruginosa genera. The optimum concentration of hexadecane that allowed high growth was 2.5%. Gas chromatography results show that all strains can degrade approximately half of hexadecane in one week of incubation. All of the strains have alkane hydroxylase gene which are important for biodegradation. As a result, this study indicates that there is a high diversity of degradative bacteria in petroleum reservoir waste water in Iran.
authors.affiliation: Department of Biology, Faculty of Sciences, Shahid Bahonar University of Kerman, Kerman, Iran.
authors.forename: Mehdi
authors.initials: M
authors.lastname: Hassanshahian
authors.forename: Mohammad
authors.initials: M
authors.lastname: Ahmadinejad
authors.forename: Hamid
authors.initials: H
authors.lastname: Tebyanian
authors.forename: Ashraf
authors.initials: A
authors.lastname: Kariminik
mesh_terms: D000473:Alkanes / Q000032:analysis / Q000378:metabolism*; D001419:Bacteria / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D001483:Base Sequence; D001673:Biodegradation, Environmental; D042926:Cytochrome P-450 CYP4A / Q000235:genetics / Q000378:metabolism; D004784:Environmental Monitoring; D007492:Iran; D008969:Molecular Sequence Data; D010578:Petroleum / Q000378:metabolism / Q000382:microbiology; D062065:Wastewater / Q000737:chemistry / Q000382:microbiology*; D014874:Water Pollutants, Chemical / Q000032:analysis / Q000378:metabolism*
chemical_list: D000473:Alkanes; D042926:Cytochrome P-450 CYP4A; D010578:Petroleum; D062065:Wastewater; D014874:Water Pollutants, Chemical
keywords: Alkane; Bacteria; Biodegradation; Iran