title: Protective effects of triterpenoids from Ganoderma resinaceum on H₂O₂-induced toxicity in HepG2 cells.
abstract: Ganoderma resinaceum Boud. (Polyporeseae) has long been used for antioxidant, immunoregulation and liver protection. From the fruiting bodies of G. resinaceum, eight new lanostanoids, lucidones D-G (1-4), 7-oxo-ganoderic acid Z2 (5), 7-oxo-ganoderic acid Z3 (6), ganoderesin A (7), and ganoderesin B (8), together with six known lanostanoids (9-14) were isolated. The structures of new compounds were elucidated through extensive spectroscopic analysis. In an in vitro model, ganoderesin B (8), ganoderol B (10) and lucidone A (11) showed inhibitory effects against the increase of ALT and AST levels in HepG2 cells induced by H2O2 compared to a control group in the range of their maximum non-toxic concentration (MNTC). However, compounds 8, 10 and 11 displayed no anti-oxidant activities by DPPH assay. Meanwhile, activation for PXR (Pregnane X Receptor) of ganoderesin B (8), ganoderol B (10) and lucidone A (11) was evaluated; ganoderol (10) exhibited a vital activation for PXR-induced CYP3A4 expression. These results suggested that GTs (Ganoderma triterpenoids) exhibited hepatoprotective activities by lowering ALT and AST levels.
authors.affiliation: State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Science, Kunming 650201, People's Republic of China.
authors.forename: Xing-Rong
authors.initials: XR
authors.lastname: Peng
authors.forename: Jie-Qing
authors.initials: JQ
authors.lastname: Liu
authors.forename: Zhong-Hui
authors.initials: ZH
authors.lastname: Han
authors.forename: Xiao-Xi
authors.initials: XX
authors.lastname: Yuan
authors.forename: Huai-Rong
authors.initials: HR
authors.lastname: Luo
authors.forename: Ming-Hua
authors.initials: MH
authors.lastname: Qiu
mesh_terms: D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D032902:Ganoderma / Q000737:chemistry; D056945:Hep G2 Cells; D006801:Humans; D006861:Hydrogen Peroxide / Q000633:toxicity*; D008099:Liver / Q000187:drug effects*; D020011:Protective Agents / Q000737:chemistry / Q000494:pharmacology*; D014315:Triterpenes / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D020011:Protective Agents; D014315:Triterpenes; D006861:Hydrogen Peroxide