title: Single-nucleotide polymorphisms and association analysis of drought-resistance gene TaSnRK2.8 in common wheat.
abstract: TaSnRK2.8, an SnRK2 (sucrose non-fermenting1-related protein kinase 2) member of wheat, confers enhanced multi-stress tolerances in carbohydrate metabolism. In the study, two types of genomic sequences of TaSnRK2.8 were detected in common wheat. Sequencing analysis showed that there was a variation-enriched region, designated TaSnRK2.8-A-C, covering the eighth intron, the ninth exon and the 3'-ﬂanking region of TaSnRK2.8-A, and no divergence occurred in TaSnRK2.8-B. Single nucleotide polymorphisms in the TaSnRK2.8-A-C region were investigated in 165 wheat accessions. Three of 751 sequenced nucleotide sites were polymorphic. Nucleotide diversity (π) in the region was 0.00068. Sliding-window analysis demonstrated that the nucleotide diversity was highest in the 3'-ﬂanking sequence. As predicted, the highly frequent SNP was significantly associated with seedling biomass under normal conditions, plant height, flag leaf width and water-soluble carbohydrate content under drought conditions. Analysis of variance of correlated traits between accessions with the A and G genotypes indicated that the A variant was the more favorable allele associated with significantly increased seedling biomass and water-soluble carbohydrates. Based on the SNP, we developed a functional marker of TaSnRK2.8-A-C, that could be utilized in wheat breeding programs aimed at improving seedling biomass and water-soluble carbohydrates, and consequently to enhance stress resistance in wheat.
authors.affiliation: College of Tobacco Science, Henan Agricultural University, Zhengzhou 450002, China.
authors.forename: Hongying
authors.initials: H
authors.lastname: Zhang
authors.forename: Xinguo
authors.initials: X
authors.lastname: Mao
authors.forename: Jianan
authors.initials: J
authors.lastname: Zhang
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Chang
authors.forename: Ruilian
authors.initials: R
authors.lastname: Jing
mesh_terms: D000222:Adaptation, Physiological / Q000235:genetics*; D000483:Alleles; D018533:Biomass; D050260:Carbohydrate Metabolism / Q000235:genetics*; D055864:Droughts; D017343:Genes, Plant; D005838:Genotype; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D020641:Polymorphism, Single Nucleotide; D017346:Protein Serine-Threonine Kinases / Q000235:genetics* / Q000378:metabolism; D036226:Seedlings / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D013312:Stress, Physiological / Q000235:genetics; D014908:Triticum / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D014867:Water
chemical_list: D010940:Plant Proteins; D014867:Water; D017346:Protein Serine-Threonine Kinases
keywords: Drought response; Single nucleotide polymorphism; Triticum aestivum