title: Stearoyl-CoA desaturase 1, elongase 6 and their fatty acid products and precursors are altered in ovariectomized rats with 17β-estradiol and progesterone treatment.
abstract: Sex differences in monounsaturated fatty acid (MUFA) levels suggest ovarian hormones may affect MUFA biosynthesis. Sprague-Dawley rats (8 weeks of age) were ovariectomized or sham operated with ovariectomized rats implanted with a constant-release hormone pellet providing 17β-estradiol, progesterone, both or neither at 10 weeks of age. After 14 days, rats were fasted overnight and sacrificed to collect plasma and livers for analysis. Hepatic stearoyl-CoA desaturase (SCD1) expression was unchanged between ovariectomized and sham controls, as determined by microarray and immunoblotting. However, SCD1 protein was increased in rats treated with estradiol plus progesterone. Elongase 6 protein levels were increased with 17β-estradiol treatment compared with sham. Rats treated with 17β-estradiol and 17β-estradiol plus progesterone had increased 16:0, 18:0, 16:1n-7 and 18:1n-7 in hepatic and plasma phospholipids. Ovarian hormones appear to be involved with MUFA biosynthesis, but the relationship appears complex and involves elongase 6 and SCD1.
authors.affiliation: Department of Kinesiology, University of Waterloo, Waterloo, ON, Canada, N2L 3G1.
authors.forename: Kristin A
authors.initials: KA
authors.lastname: Marks
authors.forename: Alex P
authors.initials: AP
authors.lastname: Kitson
authors.forename: Brittany
authors.initials: B
authors.lastname: Shaw
authors.forename: David M
authors.initials: DM
authors.lastname: Mutch
authors.forename: Ken D
authors.initials: KD
authors.lastname: Stark
mesh_terms: D000123:Acetyltransferases / Q000378:metabolism*; D000818:Animals; D004958:Estradiol / Q000494:pharmacology*; D005227:Fatty Acids / Q000378:metabolism*; D005260:Female; D015870:Gene Expression / Q000187:drug effects; D010052:Ovariectomy; D011374:Progesterone / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013230:Stearoyl-CoA Desaturase / Q000378:metabolism*
chemical_list: D005227:Fatty Acids; D011374:Progesterone; D004958:Estradiol; D013230:Stearoyl-CoA Desaturase; D000123:Acetyltransferases
keywords: Desaturase; Fatty acid synthesis; MUFA; Sex hormones