title: Applications of the hexanic fraction of Agave sisalana Perrine ex Engelm (Asparagaceae): control of inflammation and pain screening.
abstract: The present study evaluated the anti-inflammatory and analgesic properties of Agave sisalana Perrine in classic models of inflammation and pain. The hexanic fraction of A. sisalana (HFAS) was obtained by acid hydrolysis followed by hexanic reflux. Anti-inflammatory properties were examined in three acute mouse models (xylene ear oedema, hind paw oedema and pleurisy) and a chronic mouse model (granuloma cotton pellet). The antinociceptive potential was evaluated in chemical (acetic-acid) and thermal (tail-flick and hot-plate test) models of pain. When given orally, HFAS (5, 10, 25 and 50 mg/kg) reduced ear oedema (p < 0.0001; 52%, 71%, 62% and 42%, respectively). HFAS also reduced hind paw oedema at doses of 10 mg/kg and 25 mg/kg (p < 0.05; 42% and 58%, respectively) and pleurisy at doses of 10 mg/kg and 25 mg/kg (41% and 50%, respectively). In a chronic model, HFAS reduced inflammation by 46% and 58% at doses of 10 mg/kg and 25 mg/kg, respectively. Moreover, this fraction showed analgesic properties against the abdominal writhing in an acetic acid model (at doses of 5-25 mg/kg) with inhibitory rates of 24%, 54% and 48%. The HFAS also showed an increased latency time in the hot-plate (23% and 28%) and tail-flick tests (61% and 66%) for the 25 mg/kg and 50 mg/kg doses, respectively. These results suggest that HFAS has anti-inflammatory and analgesic properties.
authors.affiliation: Departamento de Biologia Estrutural e Funcional, Universidade Estadual de Campinas, Campinas, SP, Brasil. ricardo.dunder@terra.com.br
authors.forename: Ricardo José
authors.initials: RJ
authors.lastname: Dunder
authors.forename: Anderson
authors.initials: A
authors.lastname: Luiz-Ferreira
authors.forename: Ana Cristina Alves de
authors.initials: AC
authors.lastname: Almeida
authors.forename: Felipe Meira de
authors.initials: FM
authors.lastname: Faria
authors.forename: Christiane
authors.initials: C
authors.lastname: Takayama
authors.forename: Eduardo Augusto Rabelo
authors.initials: EA
authors.lastname: Socca
authors.forename: Marcos José
authors.initials: MJ
authors.lastname: Salvador
authors.forename: Gláucia Coelho
authors.initials: GC
authors.lastname: Mello
authors.forename: Catarina
authors.initials: C
authors.lastname: dos Santos
authors.forename: Pedro
authors.initials: P
authors.lastname: de Oliva-Neto
authors.forename: Alba Regina Monteiro
authors.initials: AR
authors.lastname: Souza-Brito
mesh_terms: D027882:Agave / Q000737:chemistry*; D000700:Analgesics / Q000302:isolation & purification / Q000627:therapeutic use*; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000302:isolation & purification / Q000627:therapeutic use*; D002351:Carrageenan; D004487:Edema / Q000139:chemically induced / Q000188:drug therapy; D007249:Inflammation / Q000139:chemically induced / Q000188:drug therapy*; D008297:Male; D051379:Mice; D010146:Pain / Q000139:chemically induced / Q000188:drug therapy*; D010147:Pain Measurement; D010936:Plant Extracts / Q000627:therapeutic use*; D051381:Rats
chemical_list: D000700:Analgesics; D000893:Anti-Inflammatory Agents; D010936:Plant Extracts; D002351:Carrageenan