title: In vitro O-antigen ligase assay.
abstract: WaaL is a membrane enzyme that catalyzes the glycosidic bonding of a sugar at the proximal end of the undecaprenyl-diphosphate (Und-PP)-O-antigen with a terminal sugar of the lipid A-core oligosaccharide (OS). This is a critical step in lipopolysaccharide synthesis. We describe here an assay to perform the ligation reaction in vitro utilizing native substrates.
authors.affiliation: Department of Microbiology and Immunology, Centre for Human Immunology, University of Western Ontario, London, ON, Canada.
authors.forename: Xiang
authors.initials: X
authors.lastname: Ruan
authors.forename: Miguel A
authors.initials: MA
authors.lastname: Valvano
mesh_terms: D015153:Blotting, Western / Q000379:methods; D019729:Carbon-Oxygen Ligases / Q000302:isolation & purification / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D057075:Enzyme Assays / Q000379:methods*; D004926:Escherichia coli / Q000201:enzymology* / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000302:isolation & purification / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000032:analysis / Q000378:metabolism*; D019081:O Antigens / Q000378:metabolism*; D016622:Silver Staining / Q000379:methods
chemical_list: D029968:Escherichia coli Proteins; D008070:Lipopolysaccharides; D019081:O Antigens; D019729:Carbon-Oxygen Ligases; C571195:WaaL protein, E coli