title: Effects of duodeno-jejunal bypass on glucose metabolism in obese rats with type 2 diabetes.
abstract: OBJECTIVE
  To evaluate the foregut and hindgut hypotheses for metabolic surgery in obese rats with diabetes.
  
  
  METHODS
  Otsuka Long-Evans Tokushima fatty rats were divided into a sham operation group, a partial duodeno-jejunal bypass (P-DJB) group, and a complete DJB (C-DJB) group. P-DJB is a model to test foregut hypothesis, whereas C-DJB is a model to test both hypotheses. We performed oral glucose tolerance tests (OGTT) on all groups at baseline, and then 4 and 8 weeks postoperatively. The rats were killed thereafter and the plasma levels of glucagon-like peptide-1 (GLP-1) and peptide YY (PYY) were measured. A separate sub-group of C-DJB rats underwent OGTT after treatment with the GLP-1 antagonist, the PYY antagonist, or saline.
  
  
  RESULTS
  Marked improvement of the blood glucose control during the OGTT was noted 8 weeks after C-DJB, but not 8 weeks after P-DJB or the sham operation. The serum GLP-1 and PYY levels were higher in the C-DJB group than in the other two groups. Pretreatment with the GLP-1 antagonist increased the blood glucose levels 30 min after the OGTT in the C-DJB rats.
  
  
  CONCLUSIONS
  Improvement in glucose metabolism after DJB was associated with the inflow of bile and pancreatic juice into the ileum, supporting validity of the hindgut hypothesis. GLP-1 appears to play a role in this improvement.
authors.affiliation: Division of Biological Regulation and Oncology, Department of Surgery, Tohoku University Graduate School of Medicine, 1-1 Seiryo-machi, Aoba-ku, Sendai, 980-8574, Japan.
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Imoto
authors.forename: Chikashi
authors.initials: C
authors.lastname: Shibata
authors.forename: Fumie
authors.initials: F
authors.lastname: Ikezawa
authors.forename: Daisuke
authors.initials: D
authors.lastname: Kikuchi
authors.forename: Soutoku
authors.initials: S
authors.lastname: Someya
authors.forename: Koh
authors.initials: K
authors.lastname: Miura
authors.forename: Takeshi
authors.initials: T
authors.lastname: Naitoh
authors.forename: Michiaki
authors.initials: M
authors.lastname: Unno
mesh_terms: D000818:Animals; D050110:Bariatric Surgery / Q000379:methods*; D001646:Bile / Q000378:metabolism; D001786:Blood Glucose / Q000378:metabolism; D003924:Diabetes Mellitus, Type 2 / Q000378:metabolism*; D004386:Duodenum / Q000601:surgery*; D052216:Glucagon-Like Peptide 1 / Q000097:blood / Q000502:physiology; D005947:Glucose / Q000378:metabolism*; D005951:Glucose Tolerance Test; D007082:Ileum / Q000378:metabolism; D007583:Jejunum / Q000601:surgery*; D008297:Male; D009765:Obesity / Q000378:metabolism*; D010189:Pancreatic Juice / Q000378:metabolism; D019894:Peptide YY / Q000378:metabolism / Q000502:physiology; D051381:Rats; D020334:Rats, Inbred OLETF
chemical_list: D001786:Blood Glucose; D019894:Peptide YY; D052216:Glucagon-Like Peptide 1; D005947:Glucose