title: Thymol from Thymus quinquecostatus Celak. protects against tert-butyl hydroperoxide-induced oxidative stress in Chang cells.
abstract: The present work describes the protective effects of thymol isolated from Thymus quinquecostatus Celak. against tert-butyl hydroperoxide (t-BHP)-induced oxidative damage through various experiments with Chang liver cells. Thymol significantly protected hepatocytes against t-BHP-induced cell cytotoxicity as demonstrated by increased viability. Furthermore, observation of Hoechst staining, annexin V/PI staining, and expression of Bcl-2 and Bax indicated that thymol inhibited t-BHP-induced Chang cell damage. Further, thymol inhibited the loss of mitochondrial membrane potential in t-BHP-treated Chang cells and prevented oxidative stress-triggered reactive oxygen species (ROS) and lipid peroxidation (malondialdehyde, MDA). Thymol restored the antioxidant capability of hepatocytes including glutathione (GSH) levels which were reduced by t-BHP. These results indicated that thymol prevents oxidative stress-induced damage to liver cells through suppression of ROS and MDA levels and increase of GSH level.
authors.affiliation: Department of Biotechnology, Konkuk University, Chungju, Chungbuk, 380-701, Korea.
authors.forename: Yon-Suk
authors.initials: YS
authors.lastname: Kim
authors.forename: Jin-Woo
authors.initials: JW
authors.lastname: Hwang
authors.forename: Seo-Hee
authors.initials: SH
authors.lastname: Kang
authors.forename: Ee-Hwa
authors.initials: EH
authors.lastname: Kim
authors.forename: You-Jin
authors.initials: YJ
authors.lastname: Jeon
authors.forename: Jae-Hyun
authors.initials: JH
authors.lastname: Jeong
authors.forename: Hye-Ran
authors.initials: HR
authors.lastname: Kim
authors.forename: Sang-Ho
authors.initials: SH
authors.lastname: Moon
authors.forename: Byong-Tae
authors.initials: BT
authors.lastname: Jeon
authors.forename: Pyo-Jam
authors.initials: PJ
authors.lastname: Park
mesh_terms: D017304:Annexin A5 / Q000378:metabolism; D000975:Antioxidants / Q000302:isolation & purification / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D019610:Cytoprotection; D004305:Dose-Response Relationship, Drug; D005978:Glutathione / Q000378:metabolism / Q000494:pharmacology; D022781:Hepatocytes / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D006801:Humans; D015227:Lipid Peroxidation / Q000187:drug effects; D008315:Malondialdehyde / Q000378:metabolism; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects*; D008517:Phytotherapy; D010946:Plants, Medicinal; D017382:Reactive Oxygen Species / Q000378:metabolism; D013943:Thymol / Q000302:isolation & purification / Q000494:pharmacology*; D046930:Thymus Plant / Q000737:chemistry; D051028:bcl-2-Associated X Protein / Q000378:metabolism; D020122:tert-Butylhydroperoxide / Q000633:toxicity*
chemical_list: D017304:Annexin A5; D000975:Antioxidants; C494826:BAX protein, human; D017382:Reactive Oxygen Species; D051028:bcl-2-Associated X Protein; D013943:Thymol; D008315:Malondialdehyde; D020122:tert-Butylhydroperoxide; D005978:Glutathione