title: Physicochemical characterisation of β-chitosan from Sepioteuthis lessoniana gladius.
abstract: β-Chitin and its chitosan from the gladius of Sepioteuthis lessoniana have been isolated, purified, characterised and compared with the commercial chitosan. Ash, moisture, mineral, metal and elemental content were analyzed using standard techniques. The optical activity of chitin was found to be levorotatory. The degree of deacetylation was calculated by potentiometric titration and (1)H NMR. Viscosity average molecular weight of β-chitosan was calculated by viscometry and size average molecular weight by GPC. The structure of β-chitosan was elucidated with FT-IR and NMR. Thermal nature, crystalline structure and morphology of β-chitosan were characterised through DSC, XRD and SEM, respectively. The water and fat binding capacity of β-chitosan presently studied was significantly higher than that of the commercial chitosan. The result of the present study adds that S. lessoniana gladius is also an additional source of β-chitin and chitosan of higher yield, lower molecular weight and higher degree of deacetylation.
authors.affiliation: CAS in Marine Biology, Faculty of Marine Sciences, Annamalai University, Parangipettai 608502, Tamil Nadu, India.
authors.forename: Namasivayam
authors.initials: N
authors.lastname: Subhapradha
authors.forename: Pasiyappazham
authors.initials: P
authors.lastname: Ramasamy
authors.forename: Vairamani
authors.initials: V
authors.lastname: Shanmugam
authors.forename: Perumal
authors.initials: P
authors.lastname: Madeswaran
authors.forename: Alagiri
authors.initials: A
authors.lastname: Srinivasan
authors.forename: Annaian
authors.initials: A
authors.lastname: Shanmugam
mesh_terms: D000818:Animals; D048271:Chitosan / Q000737:chemistry* / Q000302:isolation & purification; D049832:Decapodiformes / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D008970:Molecular Weight; D017550:Spectroscopy, Fourier Transform Infrared; D014783:Viscosity
chemical_list: D048271:Chitosan