title: Circadian gene Clock contributes to cell proliferation and migration of glioma and is directly regulated by tumor-suppressive miR-124.
abstract: Although the roles of circadian Clock genes and microRNAs in tumorigenesis have been profoundly studied, mechanisms of cross-talk between them in regulation of gliomagenesis are poorly understood. Here we show that the expression level of CLOCK is significantly increased in high-grade human glioma tissues and glioblastoma cell lines. In contrast miR-124 is attenuated in similar samples. Further studies show that Clock is a direct target of miR-124, and either restoration of miR-124 or silencing of CLOCK can reduce the activation of NF-κB. In conclusion, we suggest that as a target of glioma suppressor miR-124, CLOCK positively regulates glioma proliferation and migration by reinforcing NF-κB activity.
authors.affiliation: State Key Laboratory of Medical Molecular Biology, Department of Molecular Biology and Biochemistry, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100005, China.
authors.forename: Aihua
authors.initials: A
authors.lastname: Li
authors.forename: Xihua
authors.initials: X
authors.lastname: Lin
authors.forename: Xiaochao
authors.initials: X
authors.lastname: Tan
authors.forename: Bin
authors.initials: B
authors.lastname: Yin
authors.forename: Wei
authors.initials: W
authors.lastname: Han
authors.forename: Jizong
authors.initials: J
authors.lastname: Zhao
authors.forename: Jiangang
authors.initials: J
authors.lastname: Yuan
authors.forename: Boqin
authors.initials: B
authors.lastname: Qiang
authors.forename: Xiaozhong
authors.initials: X
authors.lastname: Peng
mesh_terms: D001932:Brain Neoplasms / Q000235:genetics / Q000473:pathology*; D056926:CLOCK Proteins / Q000502:physiology*; D002465:Cell Movement; D049109:Cell Proliferation; D002940:Circadian Rhythm / Q000502:physiology*; D005910:Glioma / Q000235:genetics / Q000473:pathology*; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D035683:MicroRNAs; D056926:CLOCK Proteins
keywords: CLOCK; Glioma; NF-κB; miR-124