title: Production, fractionation, characterization of extracellular polysaccharide from a newly isolated Trametes gibbosa and its hypoglycemic activity.
abstract: The submerged fermentation for extracellular polysaccharide (EPS) production from Trametes gibbosa was optimized. An optimal medium for EPS production was obtained through central composite design (CCD) as follows: 53.12 g/L maltose and 4.21 g/L polypeptone in distilled water. Furthermore, four groups of EPSs (designated as Fr-I, Fr-II, Fr-III and Fr-IV) were obtained from the culture filtrates by size exclusion chromatography (SEC), and their molecular characteristics were examined by a multiangle laser-light scattering (MALLS) and refractive index (RI) detector system. The weight-average molar mass of Fr-I was determined to be 3.872 x 10(5)g/mol and its molecular shape was revealed to be a rigid rod in an aqueous solution. Finally, the hypoglycemic effect of the EPS, investigated in streptozotocin induced diabetic mice, decreased plasma glucose, total cholesterol and triacylglycerol concentrations by 17.4%, 14.0% and 12.6%, respectively. The results indicate the potential of this EPS to prevent hyperglycemia in diabetic patients.
authors.affiliation: China Tobacco Henan Industrial Co. Ltd., Zhengzhou, Henan 450016, PR China.
authors.forename: Yuping
authors.initials: Y
authors.lastname: Ma
authors.forename: Duobin
authors.initials: D
authors.lastname: Mao
authors.forename: Lujing
authors.initials: L
authors.lastname: Geng
authors.forename: Zheng
authors.initials: Z
authors.lastname: Wang
authors.forename: Chunping
authors.initials: C
authors.lastname: Xu
mesh_terms: D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy; D005285:Fermentation; D062610:Fungal Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification / Q000627:therapeutic use; D007004:Hypoglycemic Agents / Q000737:chemistry* / Q000302:isolation & purification / Q000627:therapeutic use; D051379:Mice; D055454:Trametes / Q000737:chemistry*
chemical_list: D062610:Fungal Polysaccharides; D007004:Hypoglycemic Agents