title: Mouse strain variation in SMA(+) myofibroblast development after corneal injury.
abstract: The purpose of present study was to investigate differences in myofibroblast development after haze-generating injury in different commonly used strains of mice. The inbred mouse strains used in this study were Balb/c, C57BL/6, C3H/HeJ and DBA/1J. All mice had uniform irregular phototherapeutic keratectomy with an excimer laser according to a previously published method to generate stromal haze. DBA/1J mice generated significantly greater density of alpha smooth muscle actin (SMA)-positive myofibroblasts in the anterior stroma compared to Balb/c (p < 0.05), C57BL/6 (p < 0.05) and C3H/HeJ (p < 0.01) mice. The C3H/HeJ strain had significantly lower density of SMA-positive myofibroblasts compared to other three mouse strains. These results indicate that mouse strain must be considered in designing experiments and interpreting the results of experiments in which corneal haze and myofibroblast generation is studied in mice. Further investigation of genetics underlying mouse strain variation could provide insight into the corneal wound healing and haze generation processes.
authors.affiliation: The Cole Eye Institute, Cleveland Clinic, 9500 Euclid Ave., Cleveland, OH 44195, USA.
authors.forename: Vivek
authors.initials: V
authors.lastname: Singh
authors.forename: Andre A M
authors.initials: AA
authors.lastname: Torricelli
authors.forename: Neema
authors.initials: N
authors.lastname: Nayeb-Hashemi
authors.forename: Vandana
authors.initials: V
authors.lastname: Agrawal
authors.forename: Steven E
authors.initials: SE
authors.lastname: Wilson
mesh_terms: D000199:Actins / Q000378:metabolism*; D000818:Animals; D065306:Corneal Injuries; D003318:Corneal Opacity / Q000473:pathology*; D003319:Corneal Stroma / Q000473:pathology*; D005131:Eye Injuries / Q000378:metabolism / Q000473:pathology*; D005260:Female; D054018:Lasers, Excimer / Q000627:therapeutic use; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D058628:Myofibroblasts / Q000378:metabolism / Q000473:pathology*; D018903:Photorefractive Keratectomy; D014945:Wound Healing / Q000502:physiology
chemical_list: C558396:Acta2 protein, mouse; D000199:Actins
keywords: corneal opacity; corneal wound healing; mouse; myofibroblast; phototherapeutic keratectomy