title: Reduction of major peanut allergens Ara h 1 and Ara h 2, in roasted peanuts by ultrasound assisted enzymatic treatment.
abstract: This study investigated the effects of ultrasound, enzyme concentration and enzyme treatment time on soluble protein and major allergenic proteins (Ara h 1 and Ara h 2) of roasted peanut kernels. A 3-factor, five-level orthogonal experimental design was implemented with various ultrasonication times, concentrations of trypsin or α-chymotrypsin and treatment times. The total soluble proteins were determined by the Bicinchoninic acid (BCA) method, Ara h 1 and Ara h 2 were evaluated by SDS-PAGE and sandwich ELISA. The IgE-binding of peanut extracts was analysed by a competitive inhibition ELISA. Results indicate that ultrasound treatment, followed by protease digestion of peanuts, significantly increased the solubility of peanut protein and decreased the concentrations of Ara h 1 and Ara h 2. The sequential treatment of peanuts by ultrasonication-trypsin-alpha chymotrypsin, resulted in maximum reductions of Ara h 1/Ara h 2, and lowest IgE-binding. This study provides an approach to significantly reduce allergenic proteins in peanut product.
authors.affiliation: Center for Excellence in Post-Harvest Technologies, North Carolina Research Campus, Kannapolis, NC 28081, USA.
authors.forename: Hao
authors.initials: H
authors.lastname: Li
authors.forename: Jianmei
authors.initials: J
authors.lastname: Yu
authors.forename: Mohamed
authors.initials: M
authors.lastname: Ahmedna
authors.forename: Ipek
authors.initials: I
authors.lastname: Goktepe
mesh_terms: D055516:2S Albumins, Plant / Q000737:chemistry*; D052179:Antigens, Plant / Q000737:chemistry*; D010367:Arachis / Q000737:chemistry*; D002918:Chymotrypsin / Q000737:chemistry; D003296:Cooking; D005511:Food Handling / Q000379:methods*; D006023:Glycoproteins / Q000737:chemistry*; D008565:Membrane Proteins; D010940:Plant Proteins / Q000737:chemistry*; D013010:Sonication / Q000379:methods*; D014357:Trypsin / Q000737:chemistry
chemical_list: D055516:2S Albumins, Plant; D052179:Antigens, Plant; C500889:Ara h 1 protein, Arachis hypogaea; C500890:Ara h 2 allergen, Arachis hypogaea; D006023:Glycoproteins; D008565:Membrane Proteins; D010940:Plant Proteins; D002918:Chymotrypsin; D014357:Trypsin