title: Viral protein-coating of magnetic nanoparticles using simian virus 40 VP1.
abstract: Artificial beads including magnetite and fluorescence particles are useful to visualize pathologic tissue, such as cancers, from harmless types by magnetic resonance imaging (MRI) or fluorescence imaging. Desirable properties of diagnostic materials include high dispersion in body fluids, and the ability to target specific tissues. Here we report on the development of novel magnetic nanoparticles (MNPs) intended for use as diagnosis and therapy that are coated with viral capsid protein VP1-pentamers of simian virus 40, which are monodispersive in body fluid by conjugating epidermal growth factor (EGF) to VP1. Critically, the coating of MNPs with VP1 facilitated stable dispersion of the MNPs in body fluids. In addition, EGF was conjugated to VP1 coating on MNPs (VP1-MNPs). EGF-conjugated VP1-MNPs were successfully used to target EGF receptor-expressing tumor cells in vitro. Thus, using viral capsid protein VP1 as a coating material would be useful for medical diagnosis and therapy.
authors.affiliation: Department of Biological Information, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, Yokohama City, Kanagawa 226-8501, Japan.
authors.forename: Teruya
authors.initials: T
authors.lastname: Enomoto
authors.forename: Masaaki
authors.initials: M
authors.lastname: Kawano
authors.forename: Hajime
authors.initials: H
authors.lastname: Fukuda
authors.forename: Wataru
authors.initials: W
authors.lastname: Sawada
authors.forename: Takamasa
authors.initials: T
authors.lastname: Inoue
authors.forename: Kok Chee
authors.initials: KC
authors.lastname: Haw
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Kita
authors.forename: Satoshi
authors.initials: S
authors.lastname: Sakamoto
authors.forename: Yuki
authors.initials: Y
authors.lastname: Yamaguchi
authors.forename: Takeshi
authors.initials: T
authors.lastname: Imai
authors.forename: Mamoru
authors.initials: M
authors.lastname: Hatakeyama
authors.forename: Shigeyoshi
authors.initials: S
authors.lastname: Saito
authors.forename: Adarsh
authors.initials: A
authors.lastname: Sandhu
authors.forename: Masanori
authors.initials: M
authors.lastname: Matsui
authors.forename: Ichio
authors.initials: I
authors.lastname: Aoki
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Handa
mesh_terms: D000818:Animals; D036022:Capsid Proteins / Q000008:administration & dosage / Q000737:chemistry*; D045744:Cell Line, Tumor; D019343:Citric Acid / Q000737:chemistry; D004815:Epidermal Growth Factor / Q000008:administration & dosage / Q000737:chemistry*; D066246:ErbB Receptors / Q000378:metabolism; D006801:Humans; D007501:Iron / Q000097:blood; D060328:Magnetic Phenomena; D051379:Mice; D008807:Mice, Inbred BALB C; D053758:Nanoparticles / Q000008:administration & dosage / Q000737:chemistry*; D044967:Serum / Q000737:chemistry
chemical_list: D036022:Capsid Proteins; C062481:VP1 protein, polyomavirus; D019343:Citric Acid; D004815:Epidermal Growth Factor; D007501:Iron; D066246:ErbB Receptors
keywords: Coating; Diagnostic; Nanoparticle; Targeting; Viral capsid protein