title: The effects of EF-Ts and bismuth on EF-Tu in Helicobacter pylori: implications for an elegant timing for the introduction of EF-Ts in the elongation and EF-Tu as a potential drug target.
abstract: Helicobacter pylori is a common human pathogen responsible for various gastric diseases. Bismuth can effectively inhibit the growth of this bacterium and is commonly recommended for the treatment of the related diseases. Translation elongation factors EF-Tu and EF-Ts are two important components of the protein translation system. EF-Ts has inhibitory effects on the GTPase activity of EF-Tu and enhances GDP release, a hint that careful timing for the introduction of EF-Ts in the elongation should be accomplished to prevent the complete inhibition of the elongation process. Bismuth inhibits the chaperone activity of EF-Tu, and has opposite effects on the elongation activity: inhibitory effects on the intrinsic GTPase activity and stimulation of GDP release. The present work deepens our understanding of the bacterial elongation process as mediated by EF-Tu and EF-Ts and extends our knowledge about the inhibitory effects of bismuth-based drugs against Helicobacter pylori.
authors.affiliation: Key Laboratory of Gene Engineering of the Ministry of Education and State Key Laboratory of Biocontrol, College of Life Sciences, Sun Yat-Sen University, Guangzhou, China.
authors.forename: Dongxian
authors.initials: D
authors.lastname: Wang
authors.forename: Benping
authors.initials: B
authors.lastname: Luo
authors.forename: Weiran
authors.initials: W
authors.lastname: Shan
authors.forename: Mingcong
authors.initials: M
authors.lastname: Hao
authors.forename: Xuesong
authors.initials: X
authors.lastname: Sun
authors.forename: Ruiguang
authors.initials: R
authors.lastname: Ge
mesh_terms: D001483:Base Sequence; D001729:Bismuth / Q000494:pharmacology*; D017931:DNA Primers; D020558:GTP Phosphohydrolases / Q000378:metabolism; D016480:Helicobacter pylori / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D010444:Peptide Elongation Factor Tu / Q000378:metabolism*; D010445:Peptide Elongation Factors / Q000378:metabolism*
chemical_list: D017931:DNA Primers; D010445:Peptide Elongation Factors; C023512:elongation factor Ts; D020558:GTP Phosphohydrolases; D010444:Peptide Elongation Factor Tu; D001729:Bismuth