title: Production of D-hydantoinase via surface display and self-cleavage system.
abstract: In this study, the cell surface expression system was the first time used to directly produce extracellular enzyme. In the plasmid construction, the truncated ice nucleation protein (INP) was fused with intein (INT) and target protein, D-hydantoinase (DHTase), to form the INP-INT-DHTase gene. The plasmid containing this gene was transformed into Escherichia coli ER2566 cells. The gene construct enables the expression of INP-INT-DHTase fusion protein, which might anchor on cell membrane surface. The induction conditions were studied and optimal conditions were as follows: E. coli ER2566 was incubated at 37°C and 200 rpm till OD₆₀₀ reached 0.6. Then, 0.05 mM IPTG was added and the induction was conducted at 15°C for 24 h. The cell was harvested and resuspended in the cleavage buffer (50 mM Tris-HCl buffer, pH 6). The cleavage reaction was carried out at 25°C, and 100 rpm for 24 h. The DHTase with an activity of 0.225 U/ml and a purity of 63.2% was obtained via centrifugation. This study demonstrated the feasibility of direct extracellular enzyme production using E. coli via only two steps of centrifugation.
authors.affiliation: Department of Chemical Engineering, National Chung Hsing University, 250 Kuo-Kuang Road, Taichung 40227, Taiwan.
authors.forename: Chia-Chi
authors.initials: CC
authors.lastname: Lin
authors.forename: Tzu-Tsen
authors.initials: TT
authors.lastname: Liu
authors.forename: Shu-Chen
authors.initials: SC
authors.lastname: Kan
authors.forename: Chi-Zong
authors.initials: CZ
authors.lastname: Zang
authors.forename: Chiung-Wen
authors.initials: CW
authors.lastname: Yeh
authors.forename: Jiun-Yan
authors.initials: JY
authors.lastname: Wu
authors.forename: Jiann-Hwa
authors.initials: JH
authors.lastname: Chen
authors.forename: Chwen-Jen
authors.initials: CJ
authors.lastname: Shieh
authors.forename: Yung-Chuan
authors.initials: YC
authors.lastname: Liu
mesh_terms: D000581:Amidohydrolases / Q000096:biosynthesis / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000378:metabolism; D055162:Biocatalysis; D058675:Brevibacillus / Q000201:enzymology; D002498:Centrifugation; D004790:Enzyme Induction; D004926:Escherichia coli / Q000235:genetics; D005110:Extracellular Space / Q000201:enzymology*; D005240:Feasibility Studies; D047668:Inteins / Q000235:genetics; D042541:Intracellular Space / Q000201:enzymology; D007544:Isopropyl Thiogalactoside; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D012492:Salts; D013696:Temperature
chemical_list: D001425:Bacterial Outer Membrane Proteins; D011993:Recombinant Fusion Proteins; D012492:Salts; C063113:ice nucleation protein; D007544:Isopropyl Thiogalactoside; D000581:Amidohydrolases; C020047:dihydropyrimidinase
keywords: Biocatalysis; Enzyme production; Ice nucleation protein-intein surface display system; Protein recovery; Self-cleavage; d-Hydatoinase