title: Antioxidant and anti-inflammatory properties of Taiwanese yam (Dioscorea japonica Thunb. var. pseudojaponica (Hayata) Yamam.) and its reference compounds.
abstract: Dioscorea japonica Thunb. var. pseudojaponica (DP) is consumed as food and widely used in traditional Chinese medicine in Taiwan. The aims of this study are to investigate the antioxidant and anti-inflammatory effects of ethanol extract of DP (EDP) and its reference compounds. Fingerprint chromatogram from HPLC indicated that EDP contains gallic acid and vanillic acid. EDP was evaluated for its antioxidant effects and LPS-induced nitrite oxide (NO) production in RAW264.7 cells. EDP decreased the LPS-induced NO production and expressions of iNOS and COX-2 in RAW264.7 cells. In-vivo anti-inflammatory activities of EDP were assessed in mouse paw oedema induced by λ-carrageenan (Carr). We investigated the antioxidant mechanism of EDP via studies of the activities of catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GPx) and the levels of malondialdehyde (MDA) in the oedematous paw. The results showed that EDP might be a natural antioxidant and anti-inflammatory agent.
authors.affiliation: Nursing Department, Hsin Sheng College of Medical Care and Management, Taoyuan 325, Taiwan.
authors.forename: Chuan-Sung
authors.initials: CS
authors.lastname: Chiu
authors.forename: Jeng-Shyan
authors.initials: JS
authors.lastname: Deng
authors.forename: Heng-Yuan
authors.initials: HY
authors.lastname: Chang
authors.forename: Yi-Chuan
authors.initials: YC
authors.lastname: Chen
authors.forename: Min-Min
authors.initials: MM
authors.lastname: Lee
authors.forename: Wen-Chi
authors.initials: WC
authors.lastname: Hou
authors.forename: Chao-Ying
authors.initials: CY
authors.lastname: Lee
authors.forename: Shyh-Shyun
authors.initials: SS
authors.lastname: Huang
authors.forename: Guan-Jhong
authors.initials: GJ
authors.lastname: Huang
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage* / Q000737:chemistry; D000975:Antioxidants / Q000008:administration & dosage* / Q000737:chemistry; D002374:Catalase / Q000235:genetics / Q000378:metabolism; D027742:Dioscorea / Q000737:chemistry*; D004487:Edema / Q000188:drug therapy* / Q000276:immunology; D005979:Glutathione Peroxidase / Q000235:genetics / Q000378:metabolism; D006801:Humans; D008264:Macrophages / Q000187:drug effects / Q000201:enzymology / Q000276:immunology; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D051379:Mice; D008813:Mice, Inbred ICR; D010936:Plant Extracts / Q000008:administration & dosage* / Q000737:chemistry; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism; D013624:Taiwan
chemical_list: D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D010936:Plant Extracts; D008315:Malondialdehyde; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase