title: Phenolic-rich extract from the Costa Rican guava (Psidium friedrichsthalianum) pulp with antioxidant and anti-inflammatory activity. Potential for COPD therapy.
abstract: The potential therapeutic effects of Costa Rican guava (Psidium friedrichsthalianum) extracts for chronic obstructive pulmonary disease were examined. The ethyl acetate fraction displayed the highest antioxidant activity, as compared to the hexane, chloroform, and n-butanol fractions, as well as the crude extract. This fraction was evaluated for its anti-inflammatory activity response relationship against interleukin-8 (IL-8) and inhibition of matrix metalloproteinase-1 (MMP-1) expression before and after treatment with cigarette smoke. The ethyl acetate fraction exhibited inhibitory activity against IL-8 production and MMP-1 expression, showing the most potent inhibitory activities in both assays at 100μg/mL, and nine compounds (1-9) were found. Phenolic compounds 1-O-trans-cinnamoyl-β-d-glucopyranose (2), ellagic acid (3), myricetin (4), quercitrin (7), and quercetin (9) were identified using standard compounds or literature reports from related species. Compounds 1, 5, 6, and 8 were tentatively identified as 1,5-dimethyl citrate (1), sinapic aldehyde 4-O-β-d-glucopyranose (5), 3,3',4-tri-O-methylellagic acid-4'-O-d-glucopyranoside (6), and 1,3-O-diferuloylglycerol (8), All nine compounds are reported for the first time in Costa Rican guava.
authors.affiliation: Department of Biological Sciences, Lehman College and the Graduate Center, City University of New York, 250 Bedford Park Boulevard West, Bronx, NY 10468, USA.
authors.forename: Gema
authors.initials: G
authors.lastname: Flores
authors.forename: Keyvan
authors.initials: K
authors.lastname: Dastmalchi
authors.forename: Shi-Biao
authors.initials: SB
authors.lastname: Wu
authors.forename: Kathleen
authors.initials: K
authors.lastname: Whalen
authors.forename: Abdoulaye J
authors.initials: AJ
authors.lastname: Dabo
authors.forename: Kurt A
authors.initials: KA
authors.lastname: Reynertson
authors.forename: Robert F
authors.initials: RF
authors.lastname: Foronjy
authors.forename: Jeanine M
authors.initials: JM
authors.lastname: D Armiento
authors.forename: Edward J
authors.initials: EJ
authors.lastname: Kennelly
mesh_terms: D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D002460:Cell Line; D005638:Fruit / Q000737:chemistry; D006801:Humans; D016209:Interleukin-8 / Q000235:genetics / Q000276:immunology; D020781:Matrix Metalloproteinase 1 / Q000235:genetics / Q000276:immunology; D010636:Phenols / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D031647:Psidium / Q000737:chemistry*; D029424:Pulmonary Disease, Chronic Obstructive / Q000188:drug therapy / Q000276:immunology; D014866:Waste Products / Q000032:analysis
chemical_list: D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D016209:Interleukin-8; D010636:Phenols; D010936:Plant Extracts; D014866:Waste Products; D020781:Matrix Metalloproteinase 1