title: A novel method for the analysis of 3-mercaptopyruvate using high-performance liquid chromatography with fluorescence detection.
abstract: 3-Mercaptopyruvate (3-MP) is a metabolite of cysteine present in mammalian tissues and is known to be a substrate of 3-mercaptopyruvate sulfurtransferase (3MST, EC.3.4.1.2). The physiological relevance of the 3-MP pathway has not been fully recognized because the metabolic behavior of 3-MP remains unclear. Here, we describe a novel method using high-performance liquid chromatography with fluorescence detection to measure 3-MP formation from cysteine. To demonstrate the practical value of the present method, we applied it to analyze the 3-MP produced in biological samples from mouse tissue.
authors.affiliation: Department of Hygienic Chemistry, Meiji Pharmaceutical University, 2-522-1 Noshio, Kiyose, Tokyo 204-8588, Japan. yo@my-pharm.ac.jp
authors.forename: Yuki
authors.initials: Y
authors.lastname: Ogasawara
authors.forename: Tomoaki
authors.initials: T
authors.lastname: Hirokawa
authors.forename: Kaori
authors.initials: K
authors.lastname: Matsushima
authors.forename: Shin
authors.initials: S
authors.lastname: Koike
authors.forename: Norihiro
authors.initials: N
authors.lastname: Shibuya
authors.forename: Shinzo
authors.initials: S
authors.lastname: Tanabe
authors.forename: Kazuyuki
authors.initials: K
authors.lastname: Ishii
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism; D001923:Brain Chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003545:Cysteine / Q000031:analogs & derivatives* / Q000032:analysis / Q000378:metabolism; D007656:Ketoglutaric Acids / Q000378:metabolism; D008099:Liver / Q000737:chemistry / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence; D000637:Transaminases
chemical_list: D007656:Ketoglutaric Acids; C008366:3-mercaptopyruvic acid; D000637:Transaminases; C019968:cysteine aminotransferase; D003545:Cysteine