title: Collagen hydrolysate inhibits zymosan-induced inflammation.
abstract: During the past years, evidence accumulated showing that glycine comprises anti-inflammatory activities. These effects occur, at least in part, via the activation of glycine-gated chloride channels (GlyR). Glycine is one of the major structural units of collagen, making up about 30% of the amino acids. This study aims to investigate the anti-inflammatory potential of collagen hydrolysate (CH) using the zymosan-induced ear-skin inflammation mouse model. After oral intake of 12.5, 25 or 50 mg CH the plasma levels of glycine increased in a concentration-dependent manner. CH was able to counteract zymosan-induced ear-skin inflammation locally (ear swelling) as well as systemically (IL-6 production by lipopolysaccharide (LPS)-stimulated whole blood cells). The LPS-stimulated IL-6 production in whole blood correlated positively with the ear swelling response. This correlation was abolished by strychnine (a glycine receptor antagonist), indicating the involvement of GlyR. Collectively, these data show that CH is able to modulate inflammatory responses both locally as well as systemically. This effect might be constituted by inhibiting pro-inflammatory cytokine production via GlyR.
authors.affiliation: Danone Research, Centre for Specialised Nutrition, 6704 PH, Wageningen, the Netherlands.
authors.forename: Anita
authors.initials: A
authors.lastname: Hartog
authors.forename: Miranda
authors.initials: M
authors.lastname: Cozijnsen
authors.forename: Gerrit
authors.initials: G
authors.lastname: de Vrij
authors.forename: Johan
authors.initials: J
authors.lastname: Garssen
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003094:Collagen / Q000494:pharmacology*; D004423:Ear / Q000473:pathology; D005998:Glycine / Q000097:blood; D006868:Hydrolysis / Q000187:drug effects; D007249:Inflammation / Q000097:blood / Q000139:chemically induced / Q000473:pathology*; D015850:Interleukin-6 / Q000097:blood; D008070:Lipopolysaccharides / Q000494:pharmacology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D011492:Protein Hydrolysates / Q000494:pharmacology*; D012867:Skin / Q000187:drug effects / Q000473:pathology; D015054:Zymosan / Q000037:antagonists & inhibitors* / Q000494:pharmacology
chemical_list: D015850:Interleukin-6; D008070:Lipopolysaccharides; D011492:Protein Hydrolysates; D003094:Collagen; D015054:Zymosan; D005998:Glycine
keywords: Collagen hydrolysate; GlyR; Glycine; inflammation