title: Antitumor activity of Portulaca oleracea L. polysaccharides against cervical carcinoma in vitro and in vivo.
abstract: Portulaca oleracea L. has been used as folk medicine in different countries to treat different ailments in humans. P. oleracea L. polysaccharide (POL-P), extracted from P. oleracea L., is found to have bioactivities such as hypoglycemic and hypolipidemic activities, antioxidant and antitumor activities. In our study, a water-soluble polysaccharide (POL-P3b) was successfully purified from Galium verum L. by DEAE cellulose and Sephadex G-200 column chromatography. To evaluate the anticancer efficacy and associated mechanisms of POL-P3b on cervical cancer in vitro and in vivo, we showed that treatment of HeLa cell with POL-P3b inhibited cell proliferation. In addition, POL-P3b significantly inhibited tumor growth in U14-bearing mice. Further analysis indicated that POL-P3b possesses the activity of inhibiting cervical cancer cell growth in vitro and in vivo at a concentration- and time-dependent manner, and the mechanisms were associated with Sub-G1 phase cell cycle arrest, triggering DNA damage and inducing apoptosis.
authors.affiliation: Department of Pharmaceutical Engineering, College of Life Science & Biotechnology, Heilongjiang August First Land Reclamation University, Daqing High-Tech Industrial Development Zone, 163319, PR China.
authors.forename: Rui
authors.initials: R
authors.lastname: Zhao
authors.forename: Xu
authors.initials: X
authors.lastname: Gao
authors.forename: Yaping
authors.initials: Y
authors.lastname: Cai
authors.forename: Xingyue
authors.initials: X
authors.lastname: Shao
authors.forename: Guiyan
authors.initials: G
authors.lastname: Jia
authors.forename: Yulan
authors.initials: Y
authors.lastname: Huang
authors.forename: Xuegong
authors.initials: X
authors.lastname: Qin
authors.forename: Jingwei
authors.initials: J
authors.lastname: Wang
authors.forename: Xiaoliang
authors.initials: X
authors.lastname: Zheng
mesh_terms: D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D017209:Apoptosis / Q000187:drug effects; D002277:Carcinoma / Q000188:drug therapy*; D002453:Cell Cycle / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005260:Female; D032061:Galium / Q000737:chemistry; D006367:HeLa Cells; D006801:Humans; D051379:Mice; D008517:Phytotherapy; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D011134:Polysaccharides / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D031822:Portulaca / Q000737:chemistry*; D012995:Solubility; D002583:Uterine Cervical Neoplasms / Q000188:drug therapy*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D010936:Plant Extracts; D011134:Polysaccharides