title: Rapid dissolution of DNA in a novel bio-based ionic liquid with long-term structural and chemical stability: successful recycling of the ionic liquid for reuse in the process.
abstract: DNA from salmon testes was solubilised in two bio-based ionic liquids up to 3.5% w/w in 6 h. No structural degradation of the molecule was observed for the sample solubilised in choline-indole-3-acetate (chol-IAA). However, the molecule was found to be denatured in choline-indole-3-butyrate (chol-IBA). The structural and chemical stability of the DNA molecules after six months of storage in the former was established. Further recyclability of the ionic liquid with very high yield (90-95%) for consecutive reuse in the redissolution of DNA was demonstrated.
authors.affiliation: AcSIR-Central Salt & Marine Chemicals Research Institute, G. B. Marg, Bhavnagar-364002, Gujarat, India.
authors.forename: Chandrakant
authors.initials: C
authors.lastname: Mukesh
authors.forename: Dibyendu
authors.initials: D
authors.lastname: Mondal
authors.forename: Mukesh
authors.initials: M
authors.lastname: Sharma
authors.forename: Kamalesh
authors.initials: K
authors.lastname: Prasad
mesh_terms: D000818:Animals; D004247:DNA / Q000737:chemistry*; D052578:Ionic Liquids / Q000737:chemistry*; D008297:Male; D015394:Molecular Structure; D012474:Salmon; D013737:Testis / Q000737:chemistry*
chemical_list: D052578:Ionic Liquids; D004247:DNA