title: Genotypic diversity of polyomaviruses circulating among kidney transplant recipients in Kuwait.
abstract: BK virus (BKV) and JC virus (JCV) are human polyomaviruses that cause asymptomatic latent infections. Under immunosuppression, BKV-associated nephropathy has been documented in Kuwait and elsewhere. Even though different BKV and JCV genotypes with distinct geographical distribution have been described, the genotype of polyomavirus detected in Kuwait is still unknown. The aim of this study was to determine the genotypes of BKV and JCV detected in renal transplant recipients. The detection of polyomavirus DNA was carried out in serum and urine samples of 200 post-transplant recipients during a 1-year follow-up period. Fifty-one (25.5%) post-transplant recipients were tested positive for polyomavirus DNA by semi-nested PCR. JCV DNA could be detected in 29 (57%) patients, and BKV DNA in 22 (43%) patients. In two renal transplant recipients, both BKV and JCV were detected. According to the Bayesian phylogenetic analysis of polyomavirus VP1 sequences, the majority of detected BKV sequences were most closely related to genotypes I and IV, whereas the majority of JCV sequences were most closely related to genotype 3. Polyomavirus VP1 sequences showed strong stability for up to 12 months in most patients; however, in one patient, an amino acid substitution in the BKV VP1 protein was identified over time. The results suggest a close relationship of BKV sequences with the Asian and European strains, and of JCV sequences with the African strains. Long follow-up studies are needed to investigate the association of polyomavirus polymorphism or genotypic shift with the development of nephropathy.
authors.affiliation: Virology Unit, Department of Microbiology, Faculty of Medicine, Kuwait University, Jabriya, Kuwait. wchehadeh@hsc.edu.kw
authors.forename: Wassim
authors.initials: W
authors.lastname: Chehadeh
authors.forename: Mangalathillam Raman
authors.initials: MR
authors.lastname: Nampoory
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D001739:BK Virus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D002648:Child; D016000:Cluster Analysis; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D005260:Female; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D007577:JC Virus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D016030:Kidney Transplantation / Q000009:adverse effects*; D007730:Kuwait / Q000453:epidemiology; D008297:Male; D008875:Middle Aged; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D027601:Polyomavirus Infections / Q000453:epidemiology* / Q000821:virology; D011446:Prospective Studies; D017422:Sequence Analysis, DNA; D044967:Serum / Q000821:virology; D014180:Transplantation; D014412:Tumor Virus Infections / Q000453:epidemiology* / Q000821:virology; D014556:Urine / Q000821:virology; D055815:Young Adult
chemical_list: D004279:DNA, Viral
keywords: BK virus; JC virus; Kuwait; genotype; kidney; polyomavirus; renal transplantation