title: TNF-α triggers osteogenic differentiation of human dental pulp stem cells via the NF-κB signalling pathway.
abstract: Dental pulp stem cells (DPSCs) are a type of mesenchymal stem cells (MSCs) characterised by self-renewal and multi-lineage differentiation, including chondrocytes, adipocytes, neural cells and osteoblasts, which make it an attractive choice for tissue engineering purposes. Tumour necrosis factor α (TNF-α) had the positive effect on the mineralisation of bone marrow MSCs and stromal cells derived from human adipose tissue. However, the effect of TNF-α on DPSCs is unclear. We found that TNF-α activated the NF-κB pathway during the osteogenic differentiation of DPSCs. TNF-α also increased mineralisation and the expression of bone morphogenetic protein 2 (BMP2), alkaline phosphatase (ALP), runt-related transcription factor 2 (RUNX2) and collagen type I (COL I) during this process. PDTC, an NF-κB inhibitor, blocked the osteogenic differentiation induced by TNF-α. No effect of TNF-α on proliferation of DPSCs or cell cycle was detected. In summary, TNF-α promotes mineralisation and mineralisation-related gene expression through the NF-κB signalling pathway in DPSCs, which may provide a foundation for autologous transplantation of DPSCs.
authors.affiliation: Department of Stomatology, Affiliated Hospital of Nantong University, Nantong, China.
authors.forename: Xingmei
authors.initials: X
authors.lastname: Feng
authors.forename: Guijuan
authors.initials: G
authors.lastname: Feng
authors.forename: Jing
authors.initials: J
authors.lastname: Xing
authors.forename: Biyu
authors.initials: B
authors.lastname: Shen
authors.forename: Liren
authors.initials: L
authors.lastname: Li
authors.forename: Wei
authors.initials: W
authors.lastname: Tan
authors.forename: Yue
authors.initials: Y
authors.lastname: Xu
authors.forename: Suzhe
authors.initials: S
authors.lastname: Liu
authors.forename: Hong
authors.initials: H
authors.lastname: Liu
authors.forename: Jinxia
authors.initials: J
authors.lastname: Jiang
authors.forename: Hao
authors.initials: H
authors.lastname: Wu
authors.forename: Tao
authors.initials: T
authors.lastname: Tao
authors.forename: Zhifeng
authors.initials: Z
authors.lastname: Gu
mesh_terms: D000293:Adolescent; D000469:Alkaline Phosphatase / Q000378:metabolism; D055396:Bone Morphogenetic Protein 2 / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D024042:Collagen Type I / Q000378:metabolism; D050659:Core Binding Factor Alpha 1 Subunit / Q000378:metabolism; D003782:Dental Pulp / Q000166:cytology*; D006801:Humans; D016328:NF-kappa B / Q000037:antagonists & inhibitors / Q000378:metabolism*; D010012:Osteogenesis / Q000187:drug effects*; D011392:Proline / Q000031:analogs & derivatives / Q000494:pharmacology; D015398:Signal Transduction / Q000187:drug effects; D013234:Stem Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D013859:Thiocarbamates / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*; D055815:Young Adult
chemical_list: D055396:Bone Morphogenetic Protein 2; D024042:Collagen Type I; D050659:Core Binding Factor Alpha 1 Subunit; D016328:NF-kappa B; C493709:RUNX2 protein, human; D013859:Thiocarbamates; D014409:Tumor Necrosis Factor-alpha; C066229:prolinedithiocarbamate; D011392:Proline; D000469:Alkaline Phosphatase
keywords: dental pulp stem cells (DPSCs); nuclear factor kappaB (NF-κB); osteogenic differentiation; tumour necrosis factor α (TNF-α)