title: High frequency photoacoustic imaging for in vivo visualizing blood flow of zebrafish heart.
abstract: A technique on high frame rate(28fps), high frequency co-registered ultrasound and photoacoustic imaging for visualizing zebrafish heart blood flow was demonstrated. This approach was achieved with a 40MHz light weight(0.38g) ring-type transducer, serving as the ultrasound transmitter and receiver, to allow an optic fiber, coupled with a 532nm laser, to be inserted into the hole. From the wire target study, axial resolutions of 38µm and 42µm were obtained for ultrasound and photoacoustic imaging, respectively. Carbon nanotubes were utilized as contrast agents to increase the flow signal level by 20dB in phantom studies, and zebrafish heart blood flow was successfully observed.
authors.affiliation: NIH Resource on Medical Ultrasonic Transducer Technology, Department of Biomedical Engineering, University of Southern California, Los Angeles, CA 90089, USA.
authors.forename: Jinhyoung
authors.initials: J
authors.lastname: Park
authors.forename: Thomas M
authors.initials: TM
authors.lastname: Cummins
authors.forename: Michael
authors.initials: M
authors.lastname: Harrison
authors.forename: Jungwoo
authors.initials: J
authors.lastname: Lee
authors.forename: Qifa
authors.initials: Q
authors.lastname: Zhou
authors.forename: Ching-Ling
authors.initials: CL
authors.lastname: Lien
authors.forename: K Kirk
authors.initials: KK
authors.lastname: Shung
mesh_terms: D000818:Animals; D001783:Blood Flow Velocity / Q000502:physiology; D003326:Coronary Circulation / Q000502:physiology*; D004452:Echocardiography / Q000295:instrumentation*; D054459:Elasticity Imaging Techniques / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D055414:Myocardial Perfusion Imaging / Q000295:instrumentation*; D061088:Photoacoustic Techniques / Q000295:instrumentation*; D015203:Reproducibility of Results; D012212:Rheology / Q000295:instrumentation*; D012680:Sensitivity and Specificity; D015027:Zebrafish / Q000033:anatomy & histology / Q000502:physiology*