title: The protective effects of resveratrol on Schwann cells with toxicity induced by ethanol in vitro.
abstract: Schwann cells (SCs) are the myelin forming cells in the peripheral nervous system, they play a key role in the pathology of various polyneuropathies and provide trophic support to axons via expression of various neurotrophic factors, such as nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF) and glial cell line-derived neurotrophic factor (GDNF). Ethanol (EtOH) adversely affected both SCs proliferation and myelin formation in culture. Resveratrol (Res) has been shown to regulate many cellular processes and to display multiple protective and therapeutic effects. Whether Res has protective effects on SCs with EtOH-induced toxicity is still unclear. The protective efficacy of Res on EtOH-treated SCs in vitro was investigated in the present study. Res improved cell viability of the EtOH-treated SCs. Hoechst 33342 staining and terminal deoxynucleotidyl transferase (TdT)-mediated deoxyuridine triphosphate nick-end labeling analysis showed that the EtOH-induced apoptosis was inhibited by Res. The effects of Res were blocked by the 5'-adenosine monophosphate-activated protein kinase inhibitor Compound C and the silencing information regulator T1 inhibitor nicotinamide. Res could increase the mRNA and protein levels of BDNF and GDNF in the EtOH-treated SCs. However, the EtOH-induced increase of NGF in the SCs is inhibited by Res. The data from the present study indicate that Res protects SCs from EtOH-induced cell death and regulates the expression of neurotrophicfactors. Res and its derivative may be effective for the treatment of neuropathic diseases induced by EtOH.
authors.affiliation: Department of Anatomy, Shandong University School of Medicine, Jinan 250012, China. yuanhongtu2113@163.com
authors.forename: Hongtu
authors.initials: H
authors.lastname: Yuan
authors.forename: Jingfen
authors.initials: J
authors.lastname: Zhang
authors.forename: Huaxiang
authors.initials: H
authors.lastname: Liu
authors.forename: Zhenzhong
authors.initials: Z
authors.lastname: Li
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D001483:Base Sequence; D019208:Brain-Derived Neurotrophic Factor / Q000235:genetics; D002478:Cells, Cultured; D017931:DNA Primers; D000431:Ethanol / Q000633:toxicity*; D051100:Glial Cell Line-Derived Neurotrophic Factor / Q000235:genetics; D066298:In Vitro Techniques; D020932:Nerve Growth Factor / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar; D060888:Real-Time Polymerase Chain Reaction; D000077185:Resveratrol; D012583:Schwann Cells / Q000166:cytology / Q000187:drug effects*; D013267:Stilbenes / Q000494:pharmacology*
chemical_list: D019208:Brain-Derived Neurotrophic Factor; D017931:DNA Primers; D051100:Glial Cell Line-Derived Neurotrophic Factor; D012333:RNA, Messenger; D013267:Stilbenes; D000431:Ethanol; D020932:Nerve Growth Factor; D000077185:Resveratrol
keywords: AMP-activated protein kinase; AMPK; Apoptosis; BDNF; CC; Compound C; DMEM/F-12; Dulbecco’s Modified Eagle Medium with the F-12 supplement; EtOH; Ethanol; GDNF; NAD; NCA; NGF; Neurotrophic factors; PBS; PGC-1α; PI; ROS; Res; Resveratrol; SCs; SDS–PAGE; SIRT1; Schwann cells; TBST; Tris-buffered saline containing 0.05% Tween-20; WST-1; brain-derived neurotrophic factor; ethanol; glial cell line-derived neurotrophic factor; nerve growth factor; nicotinamide; nicotinamide adenine dinucleotide; peroxisome proliferator-activated receptor-gamma coactivator-1α; phosphate buffered saline; propidium iodide; reactive oxygen species; resveratrol; silencing information regulator T1; sodium dodecyl sulphate–polyacrylamide gel electrophoresis; water soluble tetrazolium salt-1