title: P2X3, but not P2X1, receptors mediate ATP-activated current in neurons innervating tooth-pulp.
abstract: We developed a method that allows us to label nociceptive neurons innervating tooth-pulp in rat trigeminal ganglion neurons using a retrograde fluorescence-tracing method, to record ATP-activated current in freshly isolated fluorescence-labeled neurons and to conduct single cell immunohistochemical staining for P2X1 and P2X3 subunits in the same neuron. Three types of ATP-activated current in these neurons (F, I and S) were recorded. The cells exhibiting the type F current mainly showed positive staining for P2X3, but negative staining for P2X1. The results provide direct and convincing evidence at the level of single native nociceptive neurons for correlation of the characteristics of ATP-activated currents with their composition of P2X1 and P2X3 subunits and cell size. The results also suggest that the P2X3, but not P2X1, is the main subunit that mediates the fast ATP-activated current in nociceptive neurons.
authors.affiliation: Wuhan Institutes of Biomedical Sciences, Jianghan University, Wuhan, 430056, China.|School of Medicine, Jianghan University, Wuhan, 430056, China.
authors.forename: Yu-Wei
authors.initials: YW
authors.lastname: Liu
authors.affiliation: School of Medicine, Jianghan University, Wuhan, 430056, China.
authors.forename: Xiao-Qing
authors.initials: XQ
authors.lastname: Chen
authors.affiliation: Wuhan Institutes of Biomedical Sciences, Jianghan University, Wuhan, 430056, China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Tian
authors.affiliation: Wuhan Institutes of Biomedical Sciences, Jianghan University, Wuhan, 430056, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Chen
authors.affiliation: Wuhan Institutes of Biomedical Sciences, Jianghan University, Wuhan, 430056, China.
authors.forename: Yu-Xiang
authors.initials: YX
authors.lastname: Wu
authors.affiliation: School of Medicine, Jianghan University, Wuhan, 430056, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Huang
authors.affiliation: School of Medicine, Jianghan University, Wuhan, 430056, China.
authors.forename: Hui-Ling
authors.initials: HL
authors.lastname: Yi
authors.affiliation: School of Medicine, Jianghan University, Wuhan, 430056, China.
authors.forename: Chu-Li
authors.initials: CL
authors.lastname: Yi
authors.affiliation: Wuhan Institutes of Biomedical Sciences, Jianghan University, Wuhan, 430056, China. licy.whibs@yahoo.cn.
authors.forename: Chao-Ying
authors.initials: CY
authors.lastname: Li
mesh_terms: D000200:Action Potentials / Q000502:physiology; D000255:Adenosine Triphosphate / Q000378:metabolism*; D000818:Animals; D003782:Dental Pulp / Q000294:innervation* / Q000502:physiology*; D009619:Nociceptors / Q000166:cytology / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D058474:Receptors, Purinergic P2X1 / Q000378:metabolism; D058477:Receptors, Purinergic P2X3 / Q000378:metabolism*; D014018:Tissue Distribution; D014276:Trigeminal Nerve / Q000166:cytology / Q000378:metabolism*
chemical_list: D058474:Receptors, Purinergic P2X1; D058477:Receptors, Purinergic P2X3; D000255:Adenosine Triphosphate