title: Inhibitory effect of the paraoxonase gene on the formation of rabbit coronary atherosclerosis.
abstract: OBJECTIVE
  To observe the effect on the inhibition of coronary atherosclerosis hardening of the paraoxonase gene (PON-1) which transfected to the rabbit epicardial adipose tissue.
  
  
  METHODS
  Rabbit coronary atherosclerosis model was established by high-fat feeding, liposome-encapsulated recombinant plasmid pEGFP-PON-1 50 μ L was injected to the rabbit pericardial cavity, and was harvested 4 weeks after transfection.
  
  
  RESULTS
  The epicardial fat transfected PON-1 gene had effect on the high lipid level. It significantly increased expression of PON-1 in peripheral arterial vascular tissue (P <0.05); and significantly reduced total cholesterol and low-density lipoprotein cholesterol levels (P<0.05), and the thickness ratio of coronary artery intima/media (P <0.05).
  
  
  CONCLUSIONS
  The injection of the PON-1 gene in the pericardial cavity can effectively suppress the formation of coronary atherosclerosis.
authors.affiliation: Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, 430060, China. Jingbaicn@yahoo.com.cn
authors.forename: Jing
authors.initials: J
authors.lastname: Bai
authors.forename: Hui
authors.initials: H
authors.lastname: Zhou
authors.forename: Xin-Hong
authors.initials: XH
authors.lastname: Yang
authors.forename: Hua-Fen
authors.initials: HF
authors.lastname: Liu
authors.forename: Yan-Yan
authors.initials: YY
authors.lastname: Meng
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D043303:Aryldialkylphosphatase / Q000008:administration & dosage / Q000235:genetics* / Q000494:pharmacology; D002784:Cholesterol / Q000378:metabolism; D003324:Coronary Artery Disease / Q000235:genetics / Q000517:prevention & control*; D015316:Genetic Therapy / Q000379:methods; D007267:Injections; D008297:Male; D011817:Rabbits; D011897:Random Allocation; D014162:Transfection; D014280:Triglycerides / Q000378:metabolism
chemical_list: D014280:Triglycerides; D002784:Cholesterol; D043303:Aryldialkylphosphatase; C560867:PON1 protein, mouse