title: Isolation of a novel orthobunyavirus (Brazoran virus) with a 1.7 kb S segment that encodes a unique nucleocapsid protein possessing two putative functional domains.
abstract: In July, 2012 three isolations were made from mosquitoes collected in Brazoria, Orange and Montgomery counties, Texas, USA. Data from immunofluorescence testing suggested that these isolates are members of the genus Orthobunyavirus. Expanded analyses confirmed that these isolates comprise three independent isolations of the same virus; a novel orthobunyavirus. The genetic organization of the M and L segments of this virus is similar to that of other orthobunyaviruses. However, the S segment (~1.7 kb) is nearly twice the length of known orthobunyavirus S segments, encoding a significantly larger nucleocapsid, N (~50 kDa) and putative non-structural NSs (~20 kDa) proteins in a novel strategy by which the NSs ORF precedes the N ORF. The N protein appears to consist of two functional domains; an amino portion that possesses motifs similar to other orthobunyavirus N proteins and a carboxyl portion that possesses a glutamine-rich domain with no known homologue among Bunyaviridae.
authors.affiliation: Division of Vector-Borne Infectious Diseases, National Center for Zoonotic, Vector-Borne and Enteric Diseases, Centers for Disease Control and Prevention, Public Health Service, U.S. Department of Health and Human Services, Fort Collins, CO 80521, USA. rslanciotti@cdc.gov
authors.forename: Robert S
authors.initials: RS
authors.lastname: Lanciotti
authors.forename: Olga I
authors.initials: OI
authors.lastname: Kosoy
authors.forename: Angela M
authors.initials: AM
authors.lastname: Bosco-Lauth
authors.forename: Jan
authors.initials: J
authors.lastname: Pohl
authors.forename: Olga
authors.initials: O
authors.lastname: Stuchlik
authors.forename: Matthew
authors.initials: M
authors.lastname: Reed
authors.forename: Amy J
authors.initials: AJ
authors.lastname: Lambert
mesh_terms: D000818:Animals; D016000:Cluster Analysis; D009033:Culicidae / Q000821:virology; D023061:Gene Order; D008970:Molecular Weight; D019590:Nucleocapsid Proteins / Q000737:chemistry / Q000235:genetics*; D016851:Orthobunyavirus / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D010802:Phylogeny; D017434:Protein Structure, Tertiary; D012367:RNA, Viral / Q000235:genetics; D017385:Sequence Homology; D026801:Synteny; D013781:Texas
chemical_list: D019590:Nucleocapsid Proteins; D012367:RNA, Viral
keywords: Arbovirus; Mosquito; Nucleocapsid; Orthobunyavirus; Phylogeny