title: Comparative proteomic analysis of peritoneal dialysate from chronic glomerulonephritis patients.
abstract: Peritoneal dialysis (PD) frequently contributes to peritoneal damage which cannot be easily identified without invasive techniques, implying the urgent need for biomarkers and revealing mechanisms. Chronic glomerulonephritis (CGN) is one of the leading causes of receiving dialysis treatment. Here, we attempted to analyze the peritoneal dialysate collected from CGN patients when they receive continuous ambulatory peritoneal dialysis (CAPD) treatment for the first time and after a year to reveal the protein changes that resulted from PD. Proteins were displayed by two-dimensional gel electrophoresis (2DE). Altered gel spots were digested followed by liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis for protein identification. Eight proteins were found to have differential expression levels between two groups. Their differential expressions were validated by Western blots in other sets of peritoneal dialysates. Proteins identified with higher levels in the first-time dialysate suggested their dominant appearance in CGN patients, while those that showed higher levels in peritoneal dialysate collected after one year may result from initial peritoneal inflammation or changes in the permeability of the peritoneum to middle-sized proteins. All the identified proteins may provide a perceptiveness of peritoneal changes caused by PD and may function as potential biomarkers or drug targets.
authors.affiliation: Institute of Chemistry, Academia Sinica, Taipei 115, Taiwan.
authors.forename: Hsin-Yi
authors.initials: HY
authors.lastname: Wu
authors.forename: Alex Chien Hwa
authors.initials: AC
authors.lastname: Liao
authors.forename: Chien-Cheng
authors.initials: CC
authors.lastname: Huang
authors.forename: Pao-Chi
authors.initials: PC
authors.lastname: Liao
authors.forename: Chih-Chiang
authors.initials: CC
authors.lastname: Chien
authors.forename: Wei-Chih
authors.initials: WC
authors.lastname: Kan
authors.forename: Hsien-Yi
authors.initials: HY
authors.lastname: Wang
mesh_terms: D015153:Blotting, Western; D002853:Chromatography, Liquid; D015314:Dialysis Solutions / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D005921:Glomerulonephritis / Q000378:metabolism*; D006801:Humans; D010530:Peritoneal Dialysis; D060066:Protein Interaction Maps; D020543:Proteome / Q000378:metabolism; D040901:Proteomics / Q000379:methods*; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry
chemical_list: D015314:Dialysis Solutions; D020543:Proteome