title: Single fluorescent probe distinguishes hydrogen peroxide and nitric oxide in cell imaging.
abstract: In combination with synthetic fluorescent probes, fluorescence microscopy has emerged as a powerful technique to investigate the production, localization, trafficking, and function of biomolecules in living systems in a noninvasive manner. Prompted by our interest in providing a molecular tool to disentangle the complicated interrelationship between H2O2 and NO in the signal transduction and oxidative pathways, our laboratory has developed a single fluorescent probe, FP-H2O2-NO, that can report H2O2, NO, and H2O2/NO with three different sets of fluorescence signal patterns. In this chapter, we provide essential information about the probe FP-H2O2-NO in order to assist researchers interested to apply our probe to investigate H2O2 and NO biology. We describe the use of FP-H2O2-NO with the representative examples of imaging both exogenous and endogenous H2O2 and NO in live Hela and RAW 264.7 macrophage cells by fluorescence microscopy.
authors.affiliation: State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, PR China.
authors.forename: Lin
authors.initials: L
authors.lastname: Yuan
authors.forename: Weiying
authors.initials: W
authors.lastname: Lin
authors.forename: Sasa
authors.initials: S
authors.lastname: Zhu
authors.forename: Kaibo
authors.initials: K
authors.lastname: Zheng
authors.forename: Longwei
authors.initials: L
authors.lastname: He
mesh_terms: D000818:Animals; D002460:Cell Line; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods; D005456:Fluorescent Dyes / Q000032:analysis* / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D006861:Hydrogen Peroxide / Q000032:analysis* / Q000378:metabolism; D051379:Mice; D008856:Microscopy, Fluorescence / Q000379:methods*; D009569:Nitric Oxide / Q000032:analysis* / Q000378:metabolism; D061848:Optical Imaging / Q000379:methods*
chemical_list: D005456:Fluorescent Dyes; D009569:Nitric Oxide; D006861:Hydrogen Peroxide