title: Porous tantalum coatings prepared by vacuum plasma spraying enhance bmscs osteogenic differentiation and bone regeneration in vitro and in vivo.
abstract: Tantalum, as a potential metallic implant biomaterial, is attracting more and more attention because of its excellent anticorrosion and biocompatibility. However, its significantly high elastic modulus and large mechanical incompatibility with bone tissue make it unsuitable for load-bearing implants. In this study, porous tantalum coatings were first successfully fabricated on titanium substrates by vacuum plasma spraying (VPS), which would exert the excellent biocompatibility of tantalum and alleviate the elastic modulus of tantalum for bone tissue. We evaluated cytocompatibility and osteogenesis activity of the porous tantalum coatings using human bone marrow stromal cells (hBMSCs) and its ability to repair rabbit femur bone defects. The morphology and actin cytoskeletons of hBMSCs were observed via electron microscopy and confocal, and the cell viability, proliferation and osteogenic differentiation potential of hBMSCs were examined quantitatively by PrestoBlue assay, Ki67 immunofluorescence assay, real-time PCR technology and ALP staining. For in vivo detection, the repaired femur were evaluated by histomorphology and double fluorescence labeling 3 months postoperation. Porous tantalum coating surfaces promoted hBMSCs adhesion, proliferation, osteogenesis activity and had better osseointegration and faster new bone formation rate than titanium coating control. Our observation suggested that the porous tantalum coatings had good biocompatibility and could enhance osseoinductivity in vitro and promote new bone formation in vivo. The porous tantalum coatings prepared by VPS is a promising strategy for bone regeneration.
authors.affiliation: The Key Laboratory of Stem Cell Biology, Institute of Health Sciences, Shanghai Institutes for Biological Sciences (SIBS), Chinese Academy of Sciences (CAS) and Shanghai Jiao Tong University School of Medicine (SJTUSM), Shanghai, PR China.
authors.forename: Ze
authors.initials: Z
authors.lastname: Tang
authors.forename: Youtao
authors.initials: Y
authors.lastname: Xie
authors.forename: Fei
authors.initials: F
authors.lastname: Yang
authors.forename: Yan
authors.initials: Y
authors.lastname: Huang
authors.forename: Chuandong
authors.initials: C
authors.lastname: Wang
authors.forename: Kerong
authors.initials: K
authors.lastname: Dai
authors.forename: Xuebin
authors.initials: X
authors.lastname: Zheng
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Zhang
mesh_terms: D000818:Animals; D001861:Bone Regeneration / Q000187:drug effects*; D002448:Cell Adhesion / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects; D010012:Osteogenesis / Q000187:drug effects*; D016062:Porosity; D019736:Prostheses and Implants; D011817:Rabbits; D060888:Real-Time Polymerase Chain Reaction; D013635:Tantalum / Q000737:chemistry / Q000494:pharmacology*; D023822:Tissue Engineering
chemical_list: D013635:Tantalum