title: Polymeric micelles based on poly(methacrylic acid) block-containing copolymers with different membrane destabilizing properties for cellular drug delivery.
abstract: Poly(methacrylic acid)-b-poly(ethylene oxide) are double hydrophilic block copolymers, which are able to form micelles by complexation with a counter-polycation, such as poly-l-lysine. A study was carried out on the ability of the copolymers to interact with model membranes as a function of their molecular weights and as a function of pH. Different behaviors were observed: high molecular weight copolymers respect the membrane integrity, whereas low molecular weight copolymers with a well-chosen asymmetry degree can induce a membrane alteration. Hence by choosing the appropriate molecular weight, micelles with distinct membrane interaction behaviors can be obtained leading to different intracellular traffics with or without endosomal escape, making them interesting tools for cell engineering. Especially micelles constituted of low molecular weight copolymers could exhibit the endosomal escape property, which opens vast therapeutic applications. Moreover micelles possess a homogeneous nanometric size and show variable properties of disassembly at acidic pH, of stability in physiological conditions, and finally of cyto-tolerance.
authors.affiliation: Institut Charles Gerhardt Montpellier, UMR 5253 CNRS-ENSCM-UM2-UM1, Equipe Matériaux Avancés pour la Catalyse et la Santé, 8 rue de l'Ecole Normale, 34296 Montpellier Cedex 5, France.
authors.forename: Naila
authors.initials: N
authors.lastname: Mebarek
authors.forename: Anne
authors.initials: A
authors.lastname: Aubert-Pouëssel
authors.forename: Corine
authors.initials: C
authors.lastname: Gérardin
authors.forename: Rita
authors.initials: R
authors.lastname: Vicente
authors.forename: Jean-Marie
authors.initials: JM
authors.lastname: Devoisselle
authors.forename: Sylvie
authors.initials: S
authors.lastname: Bégu
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D016503:Drug Delivery Systems; D004912:Erythrocytes / Q000187:drug effects / Q000378:metabolism; D057809:HEK293 Cells; D006454:Hemoglobins / Q000378:metabolism; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008081:Liposomes; D008567:Membranes, Artificial; D051379:Mice; D008810:Mice, Inbred C57BL; D008823:Micelles; D008970:Molecular Weight; D011092:Polyethylene Glycols / Q000737:chemistry* / Q000494:pharmacology; D011109:Polymethacrylic Acids / Q000737:chemistry* / Q000494:pharmacology
chemical_list: D006454:Hemoglobins; D008081:Liposomes; D008567:Membranes, Artificial; D008823:Micelles; D011109:Polymethacrylic Acids; C030613:polymethacrylic acid; D011092:Polyethylene Glycols
keywords: 8-hydroxypyrene-1,3,6-trisulfonic acid; Cellular trafficking; DHBC; DLS; DPPC; DPX; Double hydrophilic block copolymers; Dynamic Light Scattering; Endosomal escape; HPTS; MW; Micelles; Model membrane; PDI; PEO; PIC; PMAA; Poly(methacrylic acid) polymer; RBC; TEM; dipalmitoyl phosphatidylcholine; double hydrophilic block copolymers; molecular weights; p-xylene-bis-pyridinium bromide; poly(ethylene oxide); poly(methacrylic acid) polymers; polydispersity indice; polyion complex; red blood cells; transmission electron microscope