title: Paramyxovirus infections in ex vivo lung slice cultures of different host species.
abstract: Paramyxoviruses, including measles virus (MV), human metapneumovirus (HMPV), human respiratory syncytial virus (HRSV) and canine distemper virus (CDV), are transmitted via the respiratory route. Despite their close phylogenetic relationship, the pathogenesis of these viruses is very different. To study viral tropism and replication ex vivo, a protocol for the inflation of lungs with low-melting-point agarose mixed with culture medium was established. Lung slices were prepared and remained viable in culture at 37°C for at least 7 days. Lung slices obtained from different animal species were infected with recombinant paramyxoviruses expressing enhanced green fluorescent protein (EGFP). Progression of infection was monitored in real time by detection of EGFP fluorescence. Flow cytometric analysis of cells emigrating into the culture medium proved to be a valuable tool for qualitative and quantitative assessment of infection over time. MV replicated optimally in lung slices of macaques, HMPV and HRSV in lung slices of cotton rats, and CDV in lung slices obtained from dogs and ferrets. In conclusion, these ex vivo lung slice cultures are discriminatory models for the study of respiratory virus infections and can be used to inform the design of future in vivo experiments, thereby reducing numbers of animals required.
authors.affiliation: Department of Viroscience, Erasmus MC, University Medical Center, PO Box 2040, 3000 CA Rotterdam, The Netherlands.
authors.forename: D Tien
authors.initials: DT
authors.lastname: Nguyen
authors.forename: Rory D
authors.initials: RD
authors.lastname: de Vries
authors.forename: Martin
authors.initials: M
authors.lastname: Ludlow
authors.forename: Bernadette G
authors.initials: BG
authors.lastname: van den Hoogen
authors.forename: Ken
authors.initials: K
authors.lastname: Lemon
authors.forename: Geert
authors.initials: G
authors.lastname: van Amerongen
authors.forename: Albert D M E
authors.initials: AD
authors.lastname: Osterhaus
authors.forename: Rik L
authors.initials: RL
authors.lastname: de Swart
authors.forename: W Paul
authors.initials: WP
authors.lastname: Duprex
mesh_terms: D000818:Animals; D004285:Dogs; D008168:Lung / Q000821:virology*; D008251:Macaca; D009924:Organ Culture Techniques / Q000379:methods; D018098:Paramyxovirinae / Q000302:isolation & purification / Q000502:physiology*; D016205:Sigmodontinae; D014776:Virus Cultivation / Q000379:methods; D014779:Virus Replication
keywords: Ex vivo model; Infection; Lung slices; Paramyxovirus; Pathogenesis; Respiratory virus