title: Evaluation of triazole-chelated lanthanides as chemically stabile bioimaging agents.
abstract: Europium (Eu), dysprosium (Dy), samarium (Sm), and terbium (Tb) complexes were prepared using the neutral tridentate chelator 2,6-bis(1-benzyl-1,2,3-triazol-4-yl)pyridine and one equivalent of each lanthanide salt. The physicochemical, aerodynamic, and in vitro cellular properties of each lanthanide metal complex were studied to determine their viability as cell surface fluorescent probes. Each compound was characterized by electrospray ionization mass spectroscopy (ESI-MS), ultraperformance liquid chromatography (UPLC), differential scanning calorimetry (DSC), and thermogravimetic analysis (TGA). Upon excitation at 320 nm each complex displayed characteristic lanthanide-based fluorescence emission in the visible wavelength range with stokes shifts greater than 200 nm. Each complex was found to be chemically stable when exposed to pH range of 1-11 for 72 h and resistant to photobleaching. To simulate pulmonary administration of these fluorophores, the aerodynamic properties of the Eu and Tb complexes were determined using a next generation impactor (NGI). This measurement confirmed that the complexes retain their fluorescence emission properties after nebulization. Cellular cytotoxicity was determined on A-549 lung cancer cell line using methylthiazol tetrazolium (MTT) cytotoxicity assay at 24, 48, and 72 h postexposure to the complexes. The complexes showed a dose and time-dependent effect on the percent viability of the cells.
authors.affiliation: School of Pharmacy and Health Professions, Creighton University, Omaha, Nebraska 68178, USA.
authors.forename: Amruta
authors.initials: A
authors.lastname: Indapurkar
authors.forename: Brian
authors.initials: B
authors.lastname: Henriksen
authors.forename: Justin
authors.initials: J
authors.lastname: Tolman
authors.forename: James
authors.initials: J
authors.lastname: Fletcher
mesh_terms: D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D002614:Chelating Agents / Q000737:chemistry* / Q000633:toxicity; D056831:Coordination Complexes / Q000737:chemistry* / Q000633:toxicity; D005456:Fluorescent Dyes / Q000737:chemistry* / Q000633:toxicity; D006801:Humans; D028581:Lanthanoid Series Elements / Q000737:chemistry* / Q000633:toxicity; D012995:Solubility; D014230:Triazoles / Q000737:chemistry* / Q000633:toxicity
chemical_list: D002614:Chelating Agents; D056831:Coordination Complexes; D005456:Fluorescent Dyes; D028581:Lanthanoid Series Elements; D014230:Triazoles
keywords: cell culture; chelation; fluorescence spectroscopy; fluorescent probes; solubility; thermal analysis