title: IPS-1 plays a dual function to directly induce apoptosis in murine melanoma cells by inactivated Sendai virus.
abstract: Inactivated Sendai virus (HVJ-E) directly kills cancer cells by inducing apoptosis through a mechanism mediated by Janus kinases/signal transducers and activators of transcription (JAK/STAT) signaling pathways. However, whether other signaling pathways are involved remain largely unknown. This study aimed to investigate the mechanism underlying HVJ-E-induced apoptosis in murine B16F10 melanoma cells. We found that HVJ-E induced B16F10 cell apoptosis via the caspase pathway, particularly caspase-9, which mediates the intrinsic apoptotic pathway. Mitogen-activated protein kinase (MAPK) pathway activation also contributed to HVJ-E-induced apoptosis. Whereas caspase pathway involvement depended on both IFN-β promoter stimulator-1 (IPS-1) and type I interferon (IFN), MAPK pathway activation was independent of type I IFN but involved IPS-1. In addition, intratumoral HVJ-E treatment displayed a direct oncolytic effect in an in vivo BALB/c nude mouse melanoma model. Collectively, our data provides new insights into the mechanism underlying HVJ-E-induced apoptosis in tumor cells.
authors.affiliation: Comparative Medicine Center, College of Veterinary Medicine, Yangzhou University, Yangzhou, China.
authors.forename: Quan
authors.initials: Q
authors.lastname: Zhang
authors.forename: Xiaoshuang
authors.initials: X
authors.lastname: Xu
authors.forename: Yan
authors.initials: Y
authors.lastname: Yuan
authors.forename: Xiaocheng
authors.initials: X
authors.lastname: Gong
authors.forename: Zedong
authors.initials: Z
authors.lastname: Chen
authors.forename: Xiangming
authors.initials: X
authors.lastname: Xu
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000378:metabolism*; D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D005434:Flow Cytometry; D015151:Immunoblotting; D020287:In Situ Nick-End Labeling; D008545:Melanoma / Q000378:metabolism* / Q000473:pathology / Q000821:virology; D053078:Membrane Potential, Mitochondrial / Q000502:physiology; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D050130:Oncolytic Virotherapy / Q000379:methods*; D050504:Oncolytic Viruses / Q000502:physiology; D017382:Reactive Oxygen Species / Q000378:metabolism; D029082:Sendai virus / Q000502:physiology*; D015398:Signal Transduction / Q000502:physiology; D014162:Transfection
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D017382:Reactive Oxygen Species; C512612:MAVS protein, mouse
keywords: IFN-β promoter stimulator-1; apoptosis; inactivated Sendai virus (HVJ-E); mitogen-activated protein kinase