title: Immobilization of actively thromboresistant assemblies on sterile blood-contacting surfaces.
abstract: Rapid one-step modification of thrombomodulin with alkylamine derivatives such as azide, biotin, and PEG is achieved using an evolved sortase (eSrtA) mutant. The feasibility of a point-of-care scheme is demonstrated herein to site-specifically immobilize azido-thrombomodulin on sterilized commercial ePTFE vascular grafts, which exhibit superior thromboresistance compared with commercial heparin-coated grafts in a primate model of acute graft thrombosis.
authors.affiliation: Department of Surgery, Beth Israel Deaconess Medical Center, Harvard Medical School and the Wyss Institute of Biologically Inspired, Engineering of Harvard University, Boston, MA 02115, USA; Coulter Department of Biomedical Engineering, Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, GA 30332, USA.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Qu
authors.forename: Venkat
authors.initials: V
authors.lastname: Krishnamurthy
authors.forename: Carolyn A
authors.initials: CA
authors.lastname: Haller
authors.forename: Brent M
authors.initials: BM
authors.lastname: Dorr
authors.forename: Ulla M
authors.initials: UM
authors.lastname: Marzec
authors.forename: Sawan
authors.initials: S
authors.lastname: Hurst
authors.forename: Monica T
authors.initials: MT
authors.lastname: Hinds
authors.forename: Stephen R
authors.initials: SR
authors.lastname: Hanson
authors.forename: David R
authors.initials: DR
authors.lastname: Liu
authors.forename: Elliot L
authors.initials: EL
authors.lastname: Chaikof
mesh_terms: D000588:Amines / Q000737:chemistry* / Q000378:metabolism; D019881:Aminoacyltransferases / Q000378:metabolism; D000818:Animals; D001386:Azides / Q000737:chemistry / Q000378:metabolism; D001426:Bacterial Proteins / Q000378:metabolism; D001710:Biotin / Q000737:chemistry / Q000378:metabolism; D001792:Blood Platelets / Q000737:chemistry / Q000378:metabolism; D020099:Coated Materials, Biocompatible / Q000737:chemistry / Q000378:metabolism; D003546:Cysteine Endopeptidases / Q000378:metabolism; D004195:Disease Models, Animal; D006493:Heparin / Q000737:chemistry / Q000378:metabolism / Q000627:therapeutic use; D055767:Immobilized Proteins / Q000737:chemistry / Q000378:metabolism; D010215:Papio; D011092:Polyethylene Glycols / Q000737:chemistry / Q000378:metabolism; D011138:Polytetrafluoroethylene / Q000737:chemistry / Q000378:metabolism; D013211:Staphylococcus aureus / Q000201:enzymology; D018180:Thrombomodulin / Q000737:chemistry* / Q000378:metabolism; D013927:Thrombosis / Q000188:drug therapy
chemical_list: D000588:Amines; D001386:Azides; D001426:Bacterial Proteins; D020099:Coated Materials, Biocompatible; D055767:Immobilized Proteins; D018180:Thrombomodulin; D011092:Polyethylene Glycols; D001710:Biotin; D011138:Polytetrafluoroethylene; D006493:Heparin; D019881:Aminoacyltransferases; C120542:sortase A; D003546:Cysteine Endopeptidases
keywords: antifouling; biocompatible materials; biomedical applications; thrombomodulin; thrombosis