title: Cellular behaviour of hepatocyte-like cells from nude mouse bone marrow-derived mesenchymal stem cells on galactosylated poly(D,L-lactic-co-glycolic acid).
abstract: Previously, the galactosylation of poly(d,l-lactic-co-glycolic acid) (PLGA) surface was accomplished by grafting allylamine (AA), using inductively coupled plasma-assisted chemical vapour deposition (ICP-CVD) and conjugating lactobionic acid (LA) with AA via 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide and N-hydroxysuccinimide (EDC/NHS) activation for hepatic tissue-engineering purposes. As a continuation study, the cellular behaviour of hepatocyte-like cells (HLCs) on the surface of the galactosylated PLGA were investigated. Nude mouse bone marrow-derived mesenchymal stem cells (MSCs) were cultured under hepatogenic conditions and the differentiated cells were characterized by reverse-transcription polymerase chain reaction (RT-PCR), immunofluorescence and periodic acid-Schiff (PAS) staining. Galactosylated PLGA enhanced the proliferation rate of HLCs compared to the control; HLCs on the surface of the sample became aggregated and formed spheroids after 3 days of culture. A large number of cells on the surface of the sample exhibited increased liver-specific functional activities, such as albumin and urea secretions. In addition, multicellular spheroids in the sample strongly expressed phospholyated focal adhesion kinase (pFAK) (cell-matrix interactions), E-cadherin (cell-cell interactions) and connexin 32 (Cox32; gap junction).
authors.affiliation: Institute of Cell and Tissue Engineering, College of Medicine, Catholic University of Korea, Seoul, Republic of Korea.|Department of Biomedical Sciences, College of Medicine, Catholic University of Korea, Seoul, Republic of Korea.
authors.forename: Hyun
authors.initials: H
authors.lastname: Roh
authors.affiliation: Institute of Cell and Tissue Engineering, College of Medicine, Catholic University of Korea, Seoul, Republic of Korea.
authors.forename: Dae Hyeok
authors.initials: DH
authors.lastname: Yang
authors.affiliation: Institute of Cell and Tissue Engineering, College of Medicine, Catholic University of Korea, Seoul, Republic of Korea.|Department of Biomedical Sciences, College of Medicine, Catholic University of Korea, Seoul, Republic of Korea.
authors.forename: Heung Jae
authors.initials: HJ
authors.lastname: Chun
authors.affiliation: Department of BIN Fusion Technology, Department of Polymer-Nanoscience and Technology and Polymer BIN Research Centre, Chonbuk National University, Deokjin, Jeonju, Republic of Korea.
authors.forename: Gilson
authors.initials: G
authors.lastname: Khang
mesh_terms: D000818:Animals; D000943:Antigens, Differentiation / Q000096:biosynthesis; D001854:Bone Marrow Cells / Q000166:cytology / Q000378:metabolism*; D002454:Cell Differentiation; D022781:Hepatocytes / Q000166:cytology / Q000378:metabolism*; D019344:Lactic Acid / Q000737:chemistry*; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D051379:Mice; D008819:Mice, Nude; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D018874:Spheroids, Cellular / Q000166:cytology / Q000378:metabolism
chemical_list: D000943:Antigens, Differentiation; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid
keywords: adhesion molecule; galactosylated poly(d,l-lactic-co-glycolic acid); hepatocyte-like cell; mesenchymal stem cell; spheroid