title: Role of microRNA-21 in regulating 3T3-L1 adipocyte differentiation and adiponectin expression.
abstract: MicroRNAs are endogenous small RNAs with a high degree of conservation, participating in a variety of vital activities. In present study, to explore the effect of microRNAs on 3T3-L1 adipocyte differentiation and adiponectin expression, the adipo-related microRNAs were screened and identified by micorRNA microarray. The highly expression plasmid of microRNA-21 with obvious expression up-regulation (miR-21) and its anti-sense (miR-21 inhibitor) were constructed and transfected into 3T3-L1 preadipocytes. The effect of miR-21 on 3T3-L1 adipocyte differentiation was observed, and the protein and mRNA expression level of adiponectin and AP-1 were analyzed. Results showed that, the expression profiles of microRNAs significantly changed during 3T3-L1 adipocyte differentiation. The expression of miR-21 was obviously up-regulated. miR-21 could significantly promote adipocyte differentiation, increase adiponectin mRNA and protein expression, while decrease AP-1 protein level. Meanwhil, miR-21 inhibitor blocked the effects of miR-21 mentioned above. The overexpression of AP-1 could absolutely reverse the stimulatory effect of miR-21 on adiponectin. miR-21 plays an important role in regulating adipocyte differentiation and adiponectin expression by inhibiting AP-1 expression.
authors.affiliation: Department of Radiotherapy, First Affiliated Hospital of Guangxi Medical University, Nanning, 530021, China.
authors.forename: Min
authors.initials: M
authors.lastname: Kang
authors.forename: Li-Mei
authors.initials: LM
authors.lastname: Yan
authors.forename: Wen-Yu
authors.initials: WY
authors.lastname: Zhang
authors.forename: Yu-Mei
authors.initials: YM
authors.lastname: Li
authors.forename: An-Zhou
authors.initials: AZ
authors.lastname: Tang
authors.forename: He-Sheng
authors.initials: HS
authors.lastname: Ou
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000378:metabolism / Q000502:physiology*; D052242:Adiponectin / Q000378:metabolism*; D000818:Animals; D002454:Cell Differentiation / Q000235:genetics / Q000502:physiology*; D017931:DNA Primers / Q000235:genetics; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000235:genetics*; D051379:Mice; D035683:MicroRNAs / Q000378:metabolism*; D046228:Microarray Analysis; D018808:Transcription Factor AP-1 / Q000378:metabolism*; D014162:Transfection
chemical_list: D052242:Adiponectin; D017931:DNA Primers; C531950:MIRN21 microRNA, mouse; D035683:MicroRNAs; D018808:Transcription Factor AP-1