title: [Diverse expression of ER-α36, a novel variant of ER-α, in hippocampus and cortex of neonatal and adult rats].
abstract: ER-α36, a novel variant of ER-α, is expressed in breast, uterus, digestive tract, respiratory tract etc. The aim of the present study was to investigate the distribution and expression of ER-α36 in the central nervous system (CNS). Here, we comparatively analyzed the expression pattern of ER-α36 in the hippocampus and cortex of neonatal (1-day-old) and adult (12-week-old) Sprague-Dawley (SD) rats by using immunohistochemistry/immunocytochemistry analysis and Western blot. The results showed that ER-α36 was expressed both in hippocampus and cortex of adult rats, but mainly distributed in pyramidal neurons. ER-α36 was mainly located on the cytomembrane of hippocampal and cortical neurons from neonatal rats. Compared with the cortical neurons, the hippocampal neurons showed lower ER-α36 protein expression in the neonatal rats, but exhibited higher level of ER-α36 in the adult rats. Furthermore, the adult rats showed higher levels of ER-α36 expression in both hippocampus and cortex compared with the neonatal rats. These results suggest that ER-α36 might be involved in the regulation of membrane-initiated estrogen signaling throughout the postnatal development of diverse brain regions, and thus will be a potential target for the treatment of degenerative diseases in nervous system.
authors.affiliation: College of Life Science, Liaoning Normal University, Dalian 116081, China; The Research Center of Developmental and Educational Psychology, Liaoning Normal University, Dalian 116029, China; Regenerative Medicine Centre, First Affiliated Hospital of Dalian Medical University, Dalian 116011, China; Teacher's College, Dalian University, Dalian 116622, China; Medical College, Dalian University, Dalian 116622, China; School of Life Science, Fudan University, Shanghai 200433, China. E-mail: weizou60@126.com; liujing.dlrmc@hotmail.com.
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.forename: Chen
authors.initials: C
authors.lastname: Fang
authors.forename: Ping
authors.initials: P
authors.lastname: Zou
authors.forename: Yi-Ni
authors.initials: YN
authors.lastname: Ma
authors.forename: Dan-Nv
authors.initials: DN
authors.lastname: Han
authors.forename: Zhi-Hong
authors.initials: ZH
authors.lastname: Ji
authors.forename: Xiao-Feng
authors.initials: XF
authors.lastname: Liang
authors.forename: Xin
authors.initials: X
authors.lastname: Guan
authors.forename: Liang
authors.initials: L
authors.lastname: Huang
authors.forename: Tian
authors.initials: T
authors.lastname: Feng
authors.forename: Yi-Ting
authors.initials: YT
authors.lastname: Wang
authors.forename: Jing
authors.initials: J
authors.lastname: Liu
authors.forename: Wei
authors.initials: W
authors.lastname: Zou
mesh_terms: D000818:Animals; D002540:Cerebral Cortex / Q000378:metabolism*; D047628:Estrogen Receptor alpha / Q000378:metabolism*; D006624:Hippocampus / Q000378:metabolism*; D017966:Pyramidal Cells / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction
chemical_list: D047628:Estrogen Receptor alpha