title: Sequential injection immunoassay for human bone morphogenic protein-7 using an immunoreactor immobilized with anti-human bone morphogenic protein-7 antibody--CdSe/ZnS quantum dot conjugates.
abstract: The detection of human bone morphogenic protein-7 (BMP-7) was achieved using a sequential injection immunoassay (SIIA) system. The SIIA system is based on the binding between BMP-7 and anti-human BMP-7 (AbBMP7)-CdSe/ZnS quantum dot (QD) conjugates immobilized onto a glass disk or an optical fiber, using fluorescence detection at excitation and emission wavelengths of 470 nm and 580 nm, respectively. The AbBMP7-QD conjugates were prepared by conjugating anti-human BMP-7 antibody (AbBMP7) to hydrophilic CdSe/ZnS core/shell quantum dots (QDs). The SIIA system was fully automated using software written in the LabVIEW™ development environment. The analytical performance of the SIIA system was characterized with a number of variables such as carrier flow rate and elution buffer. Under partially optimized operating conditions, the SIIA system had a linear calibration graph at up to 10.0 ng mL(-1) BMP-7 (R(2)≥0.975) and a sample frequency of two samples per hour. The SIIA system with an optical fiber immunosensor was used to detect and quantify BMP-7 in spiked real samples obtained from a biological process with recoveries in the range of 95-102%.
authors.affiliation: School of Applied Chemical Engineering, Research Center for Biophotonics, Chonnam National University, YongBong-dong 300, GwangJu, Republic of Korea.
authors.forename: Chun-Kwang
authors.initials: CK
authors.lastname: Kim
authors.forename: Hong Dinh
authors.initials: HD
authors.lastname: Duong
authors.forename: Jong Il
authors.initials: JI
authors.lastname: Rhee
mesh_terms: D000818:Animals; D000906:Antibodies / Q000032:analysis*; D000936:Antigen-Antibody Complex / Q000032:analysis / Q000737:chemistry; D055419:Bone Morphogenetic Protein 7 / Q000032:analysis / Q000276:immunology*; D019187:Cadmium Compounds / Q000737:chemistry*; D006801:Humans; D007118:Immunoassay / Q000379:methods / Q000592:standards; D051379:Mice; D045663:Quantum Dots; D018036:Selenium Compounds / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000379:methods / Q000592:standards; D019287:Zinc Sulfate / Q000737:chemistry*
chemical_list: D000906:Antibodies; D000936:Antigen-Antibody Complex; D055419:Bone Morphogenetic Protein 7; D019187:Cadmium Compounds; D018036:Selenium Compounds; D019287:Zinc Sulfate; C058667:cadmium selenide