title: Dietary flaxseed oil and fish oil modulates expression of antioxidant and inflammatory genes with alleviation of protein glycation status and inflammation in liver of streptozotocin-nicotinamide induced diabetic rats.
abstract: Beneficial effects of dietary flaxseed oil or fish oil on streptozotocin-nicotinamide induced diabetic rats were investigated. Rats were divided into three diabetic and three non-diabetic groups and received control, flaxseed oil or fish oil diets (10%w/w). Both diets reduced blood glucose, TBARS and hepatic NO. The extent of glycation measured in terms of glycated albumin and hemoglobin was reduced significantly with both diets. Flaxseed oil diet up-regulated hepatic catalase (CAT) (activity and expression), superoxide dismutase (SOD) (activity and expression) and glutathione peroxidase (GPx) expression. Fish oil diet up-regulated hepatic CAT (activity and expression), paraoxonase-1 (PON-1) expression and down-regulated heme oxygenase-1 (HO-1) expression. Furthermore, both diets down-regulated the expression of hepatic inflammatory genes TNF-α, IL-6, MCP-1, INF-γ and NF-κB. These results were supported by histopathological observations which showed better tissue preservation in both the diets. Thus, both the diets proved to be beneficial in preventing tissue injury and alleviating diabetic insults in the livers of STZ-NIC diabetic rats.
authors.affiliation: Department of Pharmaceutical Biotechnology, Bharati Vidyapeeth Deemed University, Poona College of Pharmacy, Erandwane, Pune, Maharashtra 411 038, India.
authors.forename: Nivedita M
authors.initials: NM
authors.lastname: Jangale
authors.forename: Prasad P
authors.initials: PP
authors.lastname: Devarshi
authors.forename: Aarti A
authors.initials: AA
authors.lastname: Dubal
authors.forename: Arvindkumar E
authors.initials: AE
authors.lastname: Ghule
authors.forename: Soumya J
authors.initials: SJ
authors.lastname: Koppikar
authors.forename: Subhash L
authors.initials: SL
authors.lastname: Bodhankar
authors.forename: Ashok D
authors.initials: AD
authors.lastname: Chougale
authors.forename: Mahesh J
authors.initials: MJ
authors.lastname: Kulkarni
authors.forename: Abhay M
authors.initials: AM
authors.lastname: Harsulkar
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D043303:Aryldialkylphosphatase / Q000235:genetics / Q000378:metabolism; D002374:Catalase / Q000235:genetics / Q000378:metabolism; D016207:Cytokines / Q000235:genetics / Q000276:immunology; D003924:Diabetes Mellitus, Type 2 / Q000178:diet therapy* / Q000201:enzymology / Q000235:genetics / Q000378:metabolism; D004195:Disease Models, Animal; D005395:Fish Oils / Q000008:administration & dosage*; D005786:Gene Expression Regulation; D005947:Glucose / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000235:genetics / Q000378:metabolism; D006031:Glycosylation / Q000187:drug effects; D051547:Heme Oxygenase-1 / Q000235:genetics / Q000378:metabolism; D006454:Hemoglobins / Q000378:metabolism; D006801:Humans; D008043:Linseed Oil / Q000008:administration & dosage*; D008099:Liver / Q000201:enzymology* / Q000378:metabolism; D008297:Male; D009536:Niacinamide / Q000009:adverse effects; D051381:Rats; D017208:Rats, Wistar; D012709:Serum Albumin / Q000378:metabolism; D013311:Streptozocin / Q000009:adverse effects; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism
chemical_list: D000975:Antioxidants; D016207:Cytokines; D005395:Fish Oils; D006454:Hemoglobins; D012709:Serum Albumin; D009536:Niacinamide; D013311:Streptozocin; D008043:Linseed Oil; D002374:Catalase; D005979:Glutathione Peroxidase; D051547:Heme Oxygenase-1; D013482:Superoxide Dismutase; D043303:Aryldialkylphosphatase; D005947:Glucose