title: Pseudomonas prosekii sp. nov., a novel psychrotrophic bacterium from Antarctica.
abstract: During Czech expeditions at James Ross Island, Antarctica, in the years 2007-2009, the bacterial diversity of the genus Pseudomonas was studied. Twelve fluorescent Pseudomonas strains were isolated from various samples and were subjected to a detailed taxonomic study. A polyphasic approach included genotypic and phenotypic analyses. The genotypic analysis involved sequencing of rrs, rpoB and rpoD genes, DNA-DNA hybridization (DDH) studies as well as manual ribotyping using HindIII endonuclease. The phenotypic characterization included conventional tests as well as biotyping using the Biolog system, protein profiling by SDS-PAGE, and MALDI-TOF MS analysis. Our taxonomic study revealed that all isolates belonged to the same Pseudomonas species with psychrotrophic growth not exceeding 37 °C. The cultures showed a unique position among the phylogenetically related pseudomonads. DDH experiment between the proposed type strain of the antarctic isolates and the closest neighbour P. arsenicoxydans CCM 8423(T) showed only 40.9-50.1 % similarity, thus confirming that the characterized strains do not belong to the P. arsenicoxydans species. According to the results obtained we propose the name P. prosekii sp. nov. for this novel Pseudomonas taxon with type strain AN/28/1(T) (=CCM 7990(T) and LMG 26867(T)).
authors.affiliation: Czech Collection of Microorganisms, Masaryk University, Brno, Czech Republic, marekos@seznam.cz.
authors.forename: Marcel
authors.initials: M
authors.lastname: Kosina
authors.forename: Miloš
authors.initials: M
authors.lastname: Barták
authors.forename: Ivana
authors.initials: I
authors.lastname: Mašlaňová
authors.forename: Andrea Vávrová
authors.initials: AV
authors.lastname: Pascutti
authors.forename: Ondrej
authors.initials: O
authors.lastname: Sedo
authors.forename: Matej
authors.initials: M
authors.lastname: Lexa
authors.forename: Ivo
authors.initials: I
authors.lastname: Sedláček
mesh_terms: D000864:Antarctic Regions; D015373:Bacterial Typing Techniques; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D012321:DNA-Directed RNA Polymerases / Q000235:genetics; D004783:Environmental Microbiology; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D011549:Pseudomonas / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D021521:Ribotyping; D017422:Sequence Analysis, DNA; D012808:Sigma Factor / Q000235:genetics
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D012808:Sigma Factor; C081775:RNA polymerase sigma 70; D012321:DNA-Directed RNA Polymerases; C105078:RNA polymerase beta subunit