title: Development and validation of the liquid chromatography-tandem mass spectrometry method for quantitative estimation of candesartan from human plasma.
abstract: BACKGROUND
  A simple and sensitive liquid chromatography-tandem mass spectrometry method was developed and validated for estimation of candesartan in human plasma using the protein precipitation technique.
  
  
  METHODS
  The chromatographic separation was performed on reverse phase using a Betasil C8 (100 × 2.1 mm) 5-μm column, mobile phase of methanol:ammonium tri-floro acetate buffer with formic acid (60:40 v/v) and flow rate of 0.45 ml/min. The protonated analyte was quantitated in positive ionization by multiple reaction monitoring with a mass spectrometer. The mass transitions m/z 441.2 → 263.2 and 260.2 → 116.1 were used to measure candesartan by using propranolol as an internal standard.
  
  
  RESULTS
  The linearity of the developed method was achieved in the range of 1.2-1030 ng/ml (r(2) ≥ 0.9996) for candesartan.
  
  
  CONCLUSIONS
  The developed method is simple, rapid, accurate, cost-effective and specific; hence, it can be applied for routine analysis in pharmaceutical industries.
authors.affiliation: Department of Quality Assurance, Shri Sarvajanik Pharmacy College, Mehsana, India.
authors.forename: Shailesh T
authors.initials: ST
authors.lastname: Prajapati
authors.forename: Pratik K
authors.initials: PK
authors.lastname: Patel
authors.forename: Marmik
authors.initials: M
authors.lastname: Patel
authors.forename: Vijendra B
authors.initials: VB
authors.lastname: Chauhan
authors.forename: Chhaganbhai N
authors.initials: CN
authors.lastname: Patel
keywords: Candesaminan; LC-MS/MS method; propranolol