title: Hydrogen sulfide prevents OGD/R-induced apoptosis via improving mitochondrial dysfunction and suppressing an ROS-mediated caspase-3 pathway in cortical neurons.
abstract: Hydrogen sulfide (H2S), an endogenous gaseous mediator, has been shown to have protective effects against neuronal damage caused by brain ischemia. In this study, we explored the potential effects of H2S on oxygen-glucose deprivation/reoxygenation (OGD/R)-induced neuronal apoptosis and the possible mechanisms. We find that sodium hydrosulfide (NaHS, a donator of H2S) prevents OGD/R-induced intracellular reactive oxygen species (ROS) elevation and activation of caspase-3 in cultured mouse cortical neurons. The pretreatment of N-acetyl-l-cysteine (NAC, an ROS scavenger) also prevents OGD/R-induced activation of caspase-3. Both NaHS and NAC counteract OGD/R-induced decline in mitochondria membrane potential (MMP). Additionally, NaHS, NAC or N-Acetyl-Asp-Glu-Val-Asp-CHO (DEVD-CHO, a caspase-3 inhibitor), is shown to significantly inhibit OGD/R-induced neuronal apoptosis. These data suggest that H2S can protect against OGD/R-induced neuronal apoptosis through improving mitochondria dysfunction and suppressing an ROS-activated caspase-3 signaling pathway.
authors.affiliation: The Research Center of Neurodegenerative Diseases and Aging, Medical College of Jinggangshan University, Ji'an, China.
authors.forename: Yougen
authors.initials: Y
authors.lastname: Luo
authors.forename: Xifei
authors.initials: X
authors.lastname: Yang
authors.forename: Shenting
authors.initials: S
authors.lastname: Zhao
authors.forename: Churong
authors.initials: C
authors.lastname: Wei
authors.forename: Yedong
authors.initials: Y
authors.lastname: Yin
authors.forename: Ting
authors.initials: T
authors.lastname: Liu
authors.forename: Sainv
authors.initials: S
authors.lastname: Jiang
authors.forename: Jiajun
authors.initials: J
authors.lastname: Xie
authors.forename: Xiaomei
authors.initials: X
authors.lastname: Wan
authors.forename: Muhua
authors.initials: M
authors.lastname: Mao
authors.forename: Jiyun
authors.initials: J
authors.lastname: Wu
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3 / Q000378:metabolism*; D002540:Cerebral Cortex / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D006862:Hydrogen Sulfide / Q000494:pharmacology*; D020782:Matrix Metalloproteinases / Q000378:metabolism; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D051379:Mice; D008807:Mice, Inbred BALB C; D008928:Mitochondria / Q000187:drug effects*; D009474:Neurons / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism*
chemical_list: D017382:Reactive Oxygen Species; D053148:Caspase 3; D020782:Matrix Metalloproteinases; D006862:Hydrogen Sulfide
keywords: Apoptosis; Caspase-3; H(2)S; OGD/R; ROS