title: Convergent antibody signatures in human dengue.
abstract: Dengue is the most prevalent mosquito-borne viral disease in humans, and the lack of early prognostics, vaccines, and therapeutics contributes to immense disease burden. To identify patterns that could be used for sequence-based monitoring of the antibody response to dengue, we examined antibody heavy-chain gene rearrangements in longitudinal peripheral blood samples from 60 dengue patients. Comparing signatures between acute dengue, postrecovery, and healthy samples, we found increased expansion of B cell clones in acute dengue patients, with higher overall clonality in secondary infection. Additionally, we observed consistent antibody sequence features in acute dengue in the highly variable major antigen-binding determinant, complementarity-determining region 3 (CDR3), with specific CDR3 sequences highly enriched in acute samples compared to postrecovery, healthy, or non-dengue samples. Dengue thus provides a striking example of a human viral infection where convergent immune signatures can be identified in multiple individuals. Such signatures could facilitate surveillance of immunological memory in communities.
authors.affiliation: Division of Infectious Diseases and Vaccinology, School of Public Health, University of California, Berkeley, Berkeley, CA 94720-3370, USA.
authors.forename: Poornima
authors.initials: P
authors.lastname: Parameswaran
authors.forename: Yi
authors.initials: Y
authors.lastname: Liu
authors.forename: Krishna M
authors.initials: KM
authors.lastname: Roskin
authors.forename: Katherine K L
authors.initials: KK
authors.lastname: Jackson
authors.forename: Vaishali P
authors.initials: VP
authors.lastname: Dixit
authors.forename: Ji-Yeun
authors.initials: JY
authors.lastname: Lee
authors.forename: Karen L
authors.initials: KL
authors.lastname: Artiles
authors.forename: Simona
authors.initials: S
authors.lastname: Zompi
authors.forename: Maria José
authors.initials: MJ
authors.lastname: Vargas
authors.forename: Birgitte B
authors.initials: BB
authors.lastname: Simen
authors.forename: Bozena
authors.initials: B
authors.lastname: Hanczaruk
authors.forename: Kim R
authors.initials: KR
authors.lastname: McGowan
authors.forename: Muhammad A
authors.initials: MA
authors.lastname: Tariq
authors.forename: Nader
authors.initials: N
authors.lastname: Pourmand
authors.forename: Daphne
authors.initials: D
authors.lastname: Koller
authors.forename: Angel
authors.initials: A
authors.lastname: Balmaseda
authors.forename: Scott D
authors.initials: SD
authors.lastname: Boyd
authors.forename: Eva
authors.initials: E
authors.lastname: Harris
authors.forename: Andrew Z
authors.initials: AZ
authors.lastname: Fire
mesh_terms: D000914:Antibodies, Viral / Q000097:blood* / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology; D022801:Complementarity Determining Regions / Q000276:immunology; D003715:Dengue / Q000276:immunology*; D003716:Dengue Virus / Q000276:immunology*; D006801:Humans; D007156:Immunologic Memory
chemical_list: D000914:Antibodies, Viral; D022801:Complementarity Determining Regions