title: On the glucoside analysis: simultaneous determination of free and esterified steryl glucosides in olive oil. Detailed analysis of standards as compulsory first step.
abstract: This work covers two important gaps in the field of micronutrient databases: herein we describe a short and easy protocol that allows the analysis of both free and esterified steryl gulcosides in olive oil. By utilising accurate quantitative methods we achieve a better understanding of olive oil composition and health promoting properties. The procedure consists of isolating the fraction of interest through solid phase extraction, and using gas chromatography-flame ionisation detection for both identification and quantification of the derivatised species. Additionally, mass-spectrometry detection has been utilised for confirming the identity of the individual esterified steryl glucosides in some cases. The method's limit of detection has been set at 0.37mg/kg for each free steryl glucoside and 0.20mg/kg for each esterified steryl glucoside, whereas the recoveries are around 96% and 77%, respectively. Finally, we provide a complete analysis of the commercial standard for esterified steryl glucosides, since such information was not yet available.
authors.affiliation: Instituto de la Grasa - CSIC, Avda. Padre García Tejero 4, E-41012 Sevilla, Spain. raquel.coca@ig.csic.es
authors.forename: Raquel B
authors.initials: RB
authors.lastname: Gómez-Coca
authors.forename: María Del Carmen
authors.initials: Mdel C
authors.lastname: Pérez-Camino
authors.forename: Wenceslao
authors.initials: W
authors.lastname: Moreda
mesh_terms: D002849:Chromatography, Gas / Q000379:methods; D004951:Esterification; D005960:Glucosides / Q000737:chemistry*; D013058:Mass Spectrometry / Q000379:methods*; D015394:Molecular Structure; D000069463:Olive Oil; D010938:Plant Oils / Q000737:chemistry* / Q000592:standards; D052616:Solid Phase Extraction / Q000379:methods*
chemical_list: D005960:Glucosides; D000069463:Olive Oil; D010938:Plant Oils