title: Up-regulation of Na(+)-coupled glucose transporter SGLT1 by caveolin-1.
abstract: The Na(+)-coupled glucose transporter SGLT1 (SLC5A1) accomplishes concentrative cellular glucose uptake even at low extracellular glucose concentrations. The carrier is expressed in renal proximal tubules, small intestine and a variety of nonpolarized cells including several tumor cells. The present study explored whether SGLT1 activity is regulated by caveolin-1, which is known to regulate the insertion of several ion channels and carriers in the cell membrane. To this end, SGLT1 was expressed in Xenopus oocytes with or without additional expression of caveolin-1 and electrogenic glucose transport determined by dual electrode voltage clamp experiments. In SGLT1-expressing oocytes, but not in oocytes injected with water or caveolin-1 alone, the addition of glucose to the extracellular bath generated an inward current (Ig), which was increased following coexpression of caveolin-1. Kinetic analysis revealed that caveolin-1 increased maximal Ig without significantly modifying the glucose concentration required to trigger half maximal Ig (KM). According to chemiluminescence and confocal microscopy, caveolin-1 increased SGLT1 protein abundance in the cell membrane. Inhibition of SGLT1 insertion by brefeldin A (5μM) resulted in a decline of Ig, which was similar in the absence and presence of caveolin-1. In conclusion, caveolin-1 up-regulates SGLT1 activity by increasing carrier protein abundance in the cell membrane, an effect presumably due to stimulation of carrier protein insertion into the cell membrane.
authors.affiliation: Department of Physiology, University of Tübingen, Gmelinstr. 5, D-72076 Tübingen, Germany.
authors.forename: Bernat
authors.initials: B
authors.lastname: Elvira
authors.forename: Sabina
authors.initials: S
authors.lastname: Honisch
authors.forename: Ahmad
authors.initials: A
authors.lastname: Almilaji
authors.forename: Tatsiana
authors.initials: T
authors.lastname: Pakladok
authors.forename: Guilai
authors.initials: G
authors.lastname: Liu
authors.forename: Ekaterina
authors.initials: E
authors.lastname: Shumilina
authors.forename: Ioana
authors.initials: I
authors.lastname: Alesutan
authors.forename: Wenting
authors.initials: W
authors.lastname: Yang
authors.forename: Carlos
authors.initials: C
authors.lastname: Munoz
authors.forename: Florian
authors.initials: F
authors.lastname: Lang
mesh_terms: D000818:Animals; D051242:Caveolin 1 / Q000502:physiology*; D002462:Cell Membrane / Q000378:metabolism; D007700:Kinetics; D051269:Sodium-Glucose Transporter 1 / Q000378:metabolism / Q000502:physiology*; D015854:Up-Regulation / Q000502:physiology*; D014981:Xenopus
chemical_list: D051242:Caveolin 1; D051269:Sodium-Glucose Transporter 1
keywords: Caveolin-1; Energy depletion; Glucose uptake; Intestine; Kidney; Tumor cell