title: Simultaneous determination of free capsorubin and capsanthin in red pepper powder using u-HPLC.
abstract: A simultaneous ultra-HPLC (u-HPLC) method for the determination of free capsorubin and capsanthin in red pepper powder was validated in terms of its precision, accuracy, and linearity. The u-HPLC separation was performed on an RP C18 column (particle size 2 pm, id 2 mm length 100 mm) and with a photodiode-array detector. The recoveries of capsorubin were greater than 83.8 +/- 1.7%; the LOD and LOQ of the u-HPLC analyses were 0.043 and 0.129 mg/kg, respectively. The intraday and interday precisions for capsorubin were less than 9.01%. The recoveries of capsanthin were greater than 87.7 +/- 1.5%, and the LOD and LOQ were 0.101 and 0.306 mglkg, respectively. The intraday and interday precisions for capsanthin were less than 12.66%. All calibration curves for capsorubin and capsanthin exhibited good linearity (r2 = 0.99) within the tested ranges.
authors.affiliation: Korea Food Research Institute, Food Analysis Center, 516, Baekhyeon, Bundang, Seongnam, Gyeonggi, 463-746, Republic of Korea.
authors.forename: You-Shin
authors.initials: YS
authors.lastname: Shim
authors.forename: Ki-Jin
authors.initials: KJ
authors.lastname: Kim
authors.forename: Dongwon
authors.initials: D
authors.lastname: Seo
authors.forename: Masahito
authors.initials: M
authors.lastname: Ito
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Nakagawa
authors.forename: Shohei
authors.initials: S
authors.lastname: Arai
authors.forename: Jaeho
authors.initials: J
authors.lastname: Ha
mesh_terms: D002212:Capsicum / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D011208:Powders; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D024341:Xanthophylls / Q000737:chemistry*
chemical_list: D011208:Powders; D024341:Xanthophylls; C006889:capsanthin; C035624:capsorubin