title: Tuning DNA binding kinetics in an optical trap by plasmonic nanoparticle heating.
abstract: We report on the tuning of specific binding of DNA attached to gold nanoparticles at the individual particle pair (dimer) level in an optical trap by means of plasmonic heating. DNA hybridization events are detected optically by the change in the plasmon resonance frequency due to plasmonic coupling of the nanoparticles. We find that at larger trapping powers (i.e., larger temperatures and stiffer traps) the hybridization rates decrease by more than an order of magnitude. This result is explained by higher temperatures preventing the formation of dimers with lower binding energies. Our results demonstrate that plasmonic heating can be used to fine tune the kinetics of biomolecular binding events.
authors.affiliation: Photonics and Optoelectronics Group, Department of Physics and Center for NanoScience (CeNS), Ludwig-Maximilians-Universität München , Amalienstrasse 54, 80799 Munich, Germany.
authors.forename: Lidiya
authors.initials: L
authors.lastname: Osinkina
authors.forename: S
authors.initials: S
authors.lastname: Carretero-Palacios
authors.forename: Joachim
authors.initials: J
authors.lastname: Stehr
authors.forename: Andrey A
authors.initials: AA
authors.lastname: Lutich
authors.forename: Frank
authors.initials: F
authors.lastname: Jäckel
authors.forename: Jochen
authors.initials: J
authors.lastname: Feldmann
mesh_terms: D001665:Binding Sites; D020099:Coated Materials, Biocompatible / Q000138:chemical synthesis* / Q000528:radiation effects; D004247:DNA / Q000737:chemistry* / Q000528:radiation effects; D004735:Energy Transfer; D006046:Gold / Q000737:chemistry* / Q000528:radiation effects; D006361:Heating / Q000379:methods*; D007700:Kinetics; D008027:Light; D008422:Materials Testing; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000528:radiation effects / Q000648:ultrastructure*; D020349:Surface Plasmon Resonance / Q000379:methods*
chemical_list: D020099:Coated Materials, Biocompatible; D006046:Gold; D004247:DNA