title: One-enzyme catalyzed simultaneous plant cell disruption and conversion of released glycoside to aglycone combined with in situ product separation as green one-pot production of genipin from gardenia fruit.
abstract: A direct one-pot production of genipin, an iridoid aglycone, from crude gardenia fruit was developed. The method relied on the use of single cellulase to disrupt plant cells and to cleave off sugar molecules simultaneously, thereby enhancing the release of intracellular iridoids and converting geniposide to genipin. During the biocatalysis, eco-friendly ethyl acetate was used to extract the product, providing the partial purification and the minimization of genipin degradation. By using 10 mg/mL cellulase and 24 h-incubation at 50 °C, pH 4, combined with in situ extraction, genipin with good purity was yielded at 58.83 mg/g, which increased 12.38 and 1.72 times compared with those obtained from the procedures without either the aid of enzyme or in situ extraction, respectively. Therefore, this integrated approach is promising for the production of genipin and should be potentially applied to the preparation of other plant aglycones.
authors.affiliation: Pharmaceutical Development of Green Innovations Group (PDGIG), Faculty of Pharmacy, Silpakorn University, Nakhon Pathom 73000, Thailand.
authors.forename: Weerapath
authors.initials: W
authors.lastname: Winotapun
authors.forename: Praneet
authors.initials: P
authors.lastname: Opanasopit
authors.forename: Tanasait
authors.initials: T
authors.lastname: Ngawhirunpat
authors.forename: Theerasak
authors.initials: T
authors.lastname: Rojanarata
mesh_terms: D002480:Cellulase / Q000378:metabolism*; D032062:Gardenia / Q000737:chemistry* / Q000378:metabolism*; D057889:Iridoid Glycosides / Q000378:metabolism*; D039823:Iridoids / Q000378:metabolism*; D059828:Plant Cells / Q000737:chemistry*; D010936:Plant Extracts / Q000032:analysis / Q000737:chemistry; D013678:Technology, Pharmaceutical / Q000379:methods*
chemical_list: D057889:Iridoid Glycosides; D039823:Iridoids; D010936:Plant Extracts; C007834:genipin; D002480:Cellulase