title: Zinc-binding and structural properties of the histidine-rich loop of Arabidopsis thaliana vacuolar membrane zinc transporter MTP1.
abstract: The vacuolar Zn(2+)/H(+) antiporter of Arabidopsis thaliana, AtMTP1, has a cytosolic histidine-rich loop (His-loop). We characterized the structures and Zn(2+)-binding properties of the His-loop and other domains. Circular dichroism analyses revealed that the His-loop partly consists of a polyproline type II structure and that its conformational change is induced by Zn(2+) as well as the C-terminal domain. Isothermal titration calorimetry of the His-loop revealed a binding number of four Zn(2+) per molecule. Numbers of Ni and Co associated with the His-loop were approximately one ion per molecule and the thermodynamic parameters of the association with these ions were different from that of Zn(2+). These results suggest the involvement of the His-loop in sensing cytosolic Zn(2+) and in the regulation of zinc transport activity through Zn(2+)-induced structural change.
authors.affiliation: Laboratory of Cell Dynamics, Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya 464-8601, Japan.
authors.forename: Natsuki
authors.initials: N
authors.lastname: Tanaka
authors.forename: Miki
authors.initials: M
authors.lastname: Kawachi
authors.forename: Takashi
authors.initials: T
authors.lastname: Fujiwara
authors.forename: Masayoshi
authors.initials: M
authors.lastname: Maeshima
keywords: CTD, carboxyl terminal domain; Circular dichroism spectroscopy; His-loop, histidine-rich loop; Histidine-rich loop; ITC, isothermal titration calorimetry; Isothermal titration calorimetry; MTP, metal tolerant protein; Metal tolerance protein; NTD, amino terminal domain; PPII, polyproline type II; TFE, trifluoroethanol; TM, transmembrane.; Zinc binding; Zinc transporter