title: Effect of baicalein on the expression of VIP in extravillous cytotrophoblasts infected with human cytomegalovirus in vitro.
abstract: This paper aimed to study the ability of baicalein to block human cytomegalovirus (HCMV) infection in extravillous cytotrophoblasts (EVT) and its effect on the vasoactive intestinal peptide (VIP) expression in HCMV-infected EVT in vitro. A human trophoblast cell line (HPT-8) was chosen in this study. HCMV with 100 TCID50 was added into culture medium to infect HPT-8 cells, and then HCMV pp65 antigen was assayed by immunofluorescence staining. The infection status was determined by virus titration. Real-time quantitative polymerase chain reaction (qRT-PCR) was used to detect virus DNA load in the infected cells. The expression of VIP mRNA and protein in the infected cells was measured by qRT-PCR, immunocytochemistry and Western blotting. Concentration of VIP secreted in supernatants was determined by ELISA. Red-stained HCMV pp65 antigens were found in infected HPT-8 cells 48 h after infection. HCMV replicated in large quantity in infected HPT-8 cells 4 days after infection, reaching a peak at day 6 post-infection. After treatment with baicalein, virus DNA load in infected HPT-8 cells was decreased (P<0.05), and the levels of VIP mRNA and protein, and the concentration were raised to the normal (P>0.05). Our study suggested that baicalein exerts a positive effect on the VIP expression in HCMV-infected EVT at maternal-fetal interface.
authors.affiliation: Department of Obstetrics and Gynecology, Huazhong University of Science and Technology, Wuhan, 430030, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Qiao
authors.affiliation: Department of Pharmacy, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China.
authors.forename: Jian-Guo
authors.initials: JG
authors.lastname: Fang
authors.affiliation: Department of Obstetrics and Gynecology, Huazhong University of Science and Technology, Wuhan, 430030, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Xiao
authors.affiliation: Department of Obstetrics and Gynecology, Tai'an City Central Hospital, Tai'an, 271000, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Liu
authors.affiliation: Department of Obstetrics and Gynecology, Huazhong University of Science and Technology, Wuhan, 430030, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Liu
authors.affiliation: Department of Obstetrics and Gynecology, Huazhong University of Science and Technology, Wuhan, 430030, China.
authors.forename: Yan-Li
authors.initials: YL
authors.lastname: Zhang
authors.affiliation: Department of Obstetrics and Gynecology, Huazhong University of Science and Technology, Wuhan, 430030, China. chen_suhua@mail.hust.edu.cn.
authors.forename: Su-Hua
authors.initials: SH
authors.lastname: Chen
mesh_terms: D000998:Antiviral Agents / Q000494:pharmacology; D002460:Cell Line; D003587:Cytomegalovirus / Q000187:drug effects / Q000502:physiology*; D044950:Flavanones / Q000494:pharmacology*; D006801:Humans; D014327:Trophoblasts / Q000166:cytology / Q000187:drug effects / Q000378:metabolism* / Q000821:virology*; D014660:Vasoactive Intestinal Peptide / Q000378:metabolism*; D038301:Virus Inactivation / Q000187:drug effects
chemical_list: D000998:Antiviral Agents; D044950:Flavanones; D014660:Vasoactive Intestinal Peptide; C006680:baicalein