title: Involvement of retinoic acid-induced peroxiredoxin 6 expression in recovery of noise-induced temporary hearing threshold shifts.
abstract: All-trans retinoic acid (ATRA) is reported to reduce hair cell loss and hearing deterioration caused by noise-induced hearing loss (NIHL). The present study investigates the involvement of peroxiredoxin 6 (Prdx 6) in ATRA-mediated protection of temporary threshold shift of hearing. Mice fed with ATRA before or after exposure to white noise showed a faster recovery than untreated controls within 1 week, with a concomitant increase of cochlear Prdx 6 expression. Treatment of mouse auditory cells with ATRA induced Prdx 6 expression. A putative retinoic acid (RA)-response element (RARE) was identified in a murine Prdx 6 promoter region. Prdx 6 promoter activities were elevated in wild-type reporter plasmid-transfected cells, whereas no significant change in activity was in those with RARE-disrupted mutant reporter. RA receptor α (RARα) functions as a transactivator of Prdx 6 gene expression. These findings suggest that ATRA-induced Prdx 6 expression may be associated with rapid recovery from temporary NIHL.
authors.affiliation: Department of Otolaryngology, University of Ulsan College of Medicine, Asan Medical Center, Seoul 138-736, Republic of Korea.
authors.forename: Joong Ho
authors.initials: JH
authors.lastname: Ahn
authors.affiliation: Department of Otolaryngology (Head and Neck Surgery), Konkuk University Hospital, Seoul 143-729, Republic of Korea.
authors.forename: Jung-Eun
authors.initials: JE
authors.lastname: Shin
authors.affiliation: Asan Institute for Life Sciences, University of Ulsan College of Medicine, Asan Medical Center, Seoul 138-736, Republic of Korea.
authors.forename: Bom Yi
authors.initials: BY
authors.lastname: Chung
authors.affiliation: Asan Institute for Life Sciences, University of Ulsan College of Medicine, Asan Medical Center, Seoul 138-736, Republic of Korea.
authors.forename: Hye Mi
authors.initials: HM
authors.lastname: Lee
authors.affiliation: Asan Institute for Life Sciences, University of Ulsan College of Medicine, Asan Medical Center, Seoul 138-736, Republic of Korea.
authors.forename: Hun Hee
authors.initials: HH
authors.lastname: Kang
authors.affiliation: Department of Otolaryngology, University of Ulsan College of Medicine, Asan Medical Center, Seoul 138-736, Republic of Korea. Electronic address: jwchung@amc.seoul.kr.
authors.forename: Jong Woo
authors.initials: JW
authors.lastname: Chung
authors.affiliation: Asan Institute for Life Sciences, University of Ulsan College of Medicine, Asan Medical Center, Seoul 138-736, Republic of Korea. Electronic address: jhpak@amc.seoul.kr.
authors.forename: Jhang Ho
authors.initials: JH
authors.lastname: Pak
mesh_terms: D000818:Animals; D001305:Auditory Fatigue / Q000187:drug effects*; D001665:Binding Sites; D002460:Cell Line; D003051:Cochlea / Q000187:drug effects* / Q000378:metabolism / Q000503:physiopathology; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D006309:Hearing / Q000187:drug effects*; D006317:Hearing Loss, Noise-Induced / Q000209:etiology / Q000378:metabolism* / Q000503:physiopathology; D051379:Mice; D008807:Mice, Inbred BALB C; D009154:Mutation; D054465:Peroxiredoxin VI / Q000235:genetics / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D018168:Receptors, Retinoic Acid / Q000819:agonists / Q000235:genetics / Q000378:metabolism; D020127:Recovery of Function; D020218:Response Elements; D000072482:Retinoic Acid Receptor alpha; D015398:Signal Transduction; D013997:Time Factors; D015533:Transcriptional Activation; D014162:Transfection; D014212:Tretinoin / Q000494:pharmacology*; D015854:Up-Regulation
chemical_list: C000607072:Rara protein, mouse; D018168:Receptors, Retinoic Acid; D000072482:Retinoic Acid Receptor alpha; D014212:Tretinoin; D054465:Peroxiredoxin VI; C515983:Prdx6 protein, mouse
keywords: ATRA; All-trans retinoic acid; Cochlea; NIHL; Noise-induced temporary threshold shift; Peroxiredoxin 6; Prdx 6; RAR; RARE; Retinoic acid-response element; all trans retinoic acid; noise-induced hearing loss; peroxiredoxin 6; retinoic acid receptors; retinoic acid-response element