title: Structural characterization of an immunoenhancing glucan isolated from a mushroom Macrolepiota dolichaula.
abstract: A water soluble branched glucan (PS-I) was isolated from aqueous extract of the fruit bodies of an edible mushroom Macrolepiota dolichaula, having average molecular weight ~2.02×10(5) Da. The structure of this PS-I was determined using total hydrolysis, methylation analysis, Smith degradation, partial hydrolysis, and 1D/2D NMR experiments. Total hydrolysis and methylation analysis results showed the presence of (1→3, 6)-, (1→6)-, (1→4)-, (1→3)-linked and terminal β-D-glucopyranosyl residues in a relative proportion of nearly 1:2:1:1:1. All the chemical and NMR results indicated that the PS-I was a branched glucan, and the repeating unit of this glucan consisted of a backbone chain of three (1→6)-linked-β-D-glucopyranosyl residues where one of the backbone residues is branched at O-3 with (1→3)- moiety which is further attached to another (1→4)- residue and terminated with a non-reducing β-D-glucopyranosyl residue. The PS-I exhibited in vitro macrophage activation in RAW 264.7 cell line as well as splenocyte and thymocyte activation in mouse cell culture medium.
authors.affiliation: Department of Chemistry and Chemical Technology, Vidyasagar University, Midnapore 721102, West Bengal, India.
authors.forename: Surajit
authors.initials: S
authors.lastname: Samanta
authors.forename: Ashis K
authors.initials: AK
authors.lastname: Nandi
authors.forename: Ipsita K
authors.initials: IK
authors.lastname: Sen
authors.forename: Praloy K
authors.initials: PK
authors.lastname: Maji
authors.forename: K Sanjana P
authors.initials: KS
authors.lastname: Devi
authors.forename: Tapas K
authors.initials: TK
authors.lastname: Maiti
authors.forename: Syed S
authors.initials: SS
authors.lastname: Islam
mesh_terms: D000363:Agaricales / Q000737:chemistry*; D000818:Animals; D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D005936:Glucans / Q000737:chemistry* / Q000302:isolation & purification* / Q000494:pharmacology; D007155:Immunologic Factors / Q000737:chemistry* / Q000302:isolation & purification* / Q000494:pharmacology; D008262:Macrophage Activation / Q000187:drug effects / Q000276:immunology; D008264:Macrophages / Q000187:drug effects / Q000276:immunology; D051379:Mice; D019906:Nuclear Magnetic Resonance, Biomolecular
chemical_list: D005936:Glucans; D007155:Immunologic Factors
keywords: Branched glucan; Immunoactivation; Macrolepiota dolichaula; NMR studies