title: Optoperforation of single, intact Arabidopsis cells for uptake of extracellular dye-conjugated dextran.
abstract: A plant science research goal is to manipulate single cells in an intact organism in order to study their interactions with neighboring cells. Based on a technique previously demonstrated in isolated plant cells, mammalian cells and cyanobacteria, Arabidopsis epidermal cells were optoperforated to allow for uptake of external cascade blue-labeled dextrans. Adverse organelle responses were determined to be minimal and dye retention was demonstrated for at least 72 hours. This technique overcomes the physical challenges presented by the plant cell wall and demonstrates the feasibility of in situ optoperforation.
authors.affiliation: Department of Plant Pathology, Physiology, and Weed Science, Virginia Tech, Blacksburg, VA 24061, USA. megan00@vt.edu
authors.forename: Megan L
authors.initials: ML
authors.lastname: LeBlanc
authors.forename: Travis R
authors.initials: TR
authors.lastname: Merritt
authors.forename: Jameel
authors.initials: J
authors.lastname: McMillan
authors.forename: James H
authors.initials: JH
authors.lastname: Westwood
authors.forename: Giti A
authors.initials: GA
authors.lastname: Khodaparast
mesh_terms: D017360:Arabidopsis / Q000378:metabolism* / Q000528:radiation effects; D003911:Dextrans / Q000008:administration & dosage / Q000493:pharmacokinetics*; D004337:Drug Carriers / Q000008:administration & dosage / Q000493:pharmacokinetics; D018274:Electroporation / Q000379:methods*; D005456:Fluorescent Dyes / Q000008:administration & dosage / Q000493:pharmacokinetics*; D007834:Lasers; D019441:Plant Epidermis / Q000378:metabolism* / Q000528:radiation effects; D014018:Tissue Distribution
chemical_list: D003911:Dextrans; D004337:Drug Carriers; D005456:Fluorescent Dyes