title: Spectroscopic characterization of copper(I) binding to apo and metal-reconstituted zinc finger peptides.
abstract: Cu(I) exhibits high affinity for thiolate ligands, suggesting that thiol-rich zinc or iron binding sites may be subject to disruption during copper stress conditions. Zinc fingers constitute a large class of metalloproteins that use a combination of cysteine and histidine residues that bind Zn(II) as a structural element. Despite the shared preference of both copper and zinc for thiolate and amine coordination, the susceptibility of zinc finger domains toward copper substitution is not well studied. We report spectroscopic studies that characterize the Cu(I) binding properties of the zinc finger consensus peptides CP-CCHH, CP-CCHC, and CP-CCCC and the C-terminal zinc finger domain of HIV-1 nucleocapsid protein p7 (NCp7_C). Cu(I) binds to both the apopeptides and the Co(II)-substituted peptides, and the stoichiometry of Cu(I) binding is dependent on the number of cysteine thiols at the metal binding site. Fluorescence studies of the Zn(II)-NCp7_C complex indicate that Cu(I) also effectively competes with Zn(II) at the metal binding site, despite the high affinity of Zn(II) for the CCHC binding motif. Circular dichroism studies on both CP-CCHC and NCp7_C show that the conformations of the Cu(I)-bound complexes differ substantially from those of the Zn(II) species, implying that Cu(I) substitution is likely to impact zinc finger function. These results show that for the peptides studied here, Cu(I) is the thermodynamically favored metal despite the known high Zn(II) affinity of zinc finger domains, suggesting that Cu(I)-substituted zinc finger domains might be relevant in the context of both copper toxicity mechanisms and copper-responsive transcription factors.
authors.affiliation: Department of Chemistry, Macalester College, 1600 Grand Avenue, Saint Paul, MN 55105, USA.
authors.forename: Reginald T
authors.initials: RT
authors.lastname: Doku
authors.forename: Grace
authors.initials: G
authors.lastname: Park
authors.forename: Korin E
authors.initials: KE
authors.lastname: Wheeler
authors.forename: Kathryn E
authors.initials: KE
authors.lastname: Splan
mesh_terms: D001059:Apoproteins / Q000737:chemistry*; D001665:Binding Sites; D002942:Circular Dichroism; D003300:Copper / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013816:Thermodynamics; D016335:Zinc Fingers; D054301:gag Gene Products, Human Immunodeficiency Virus / Q000737:chemistry*
chemical_list: D001059:Apoproteins; C071884:NCP7 protein, Human immunodeficiency virus 1; D010455:Peptides; D054301:gag Gene Products, Human Immunodeficiency Virus; D003300:Copper