title: Co-segregation analysis and mapping of the anthracnose Co-10 and angular leaf spot Phg-ON disease-resistance genes in the common bean cultivar Ouro Negro.
abstract: Anthracnose (ANT) and angular leaf spot (ALS) are devastating diseases of common bean (Phaseolus vulgaris L.). Ouro Negro is a highly productive common bean cultivar, which contains the Co-10 and Phg-ON genes for resistance to ANT and ALS, respectively. In this study, we performed a genetic co-segregation analysis of resistance to ANT and ALS using an F2 population from the Rudá × Ouro Negro cross and the F2:3 families from the AND 277 × Ouro Negro cross. Ouro Negro is resistant to races 7 and 73 of the ANT and race 63-39 of the ALS pathogens. Conversely, cultivars AND 277 and Rudá are susceptible to races 7 and 73 of ANT, respectively. Both cultivars are susceptible to race 63-39 of ALS. Co-segregation analysis revealed that Co-10 and Phg-ON were inherited together, conferring resistance to races 7 and 73 of ANT and race 63-39 of ALS. The Co-10 and Phg-ON genes were co-segregated and were tightly linked at a distance of 0.0 cM on chromosome Pv04. The molecular marker g2303 was linked to Co-10 and Phg-ON at a distance of 0.0 cM. Because of their physical linkage in a cis configuration, the Co-10 and Phg-ON resistance alleles are inherited together and can be monitored with great efficiency using g2303. The close linkage between the Co-10 and Phg-ON genes and prior evidence are consistent with the existence of a resistance gene cluster at one end of chromosome Pv04, which also contains the Co-3 locus and ANT resistance quantitative trait loci. These results will be very useful for breeding programs aimed at developing bean cultivars with ANT and ALS resistance using marker-assisted selection.
authors.affiliation: Departamento de Agronomia, Universidade Estadual de Maringá, Av. Colombo 5790, Maringá, Paraná, Brazil.
authors.forename: M C
authors.initials: MC
authors.lastname: Gonçalves-Vidigal
authors.forename: A S
authors.initials: AS
authors.lastname: Cruz
authors.forename: G F
authors.initials: GF
authors.lastname: Lacanallo
authors.forename: P S
authors.initials: PS
authors.lastname: Vidigal Filho
authors.forename: L L
authors.initials: LL
authors.lastname: Sousa
authors.forename: C M N A
authors.initials: CM
authors.lastname: Pacheco
authors.forename: P
authors.initials: P
authors.lastname: McClean
authors.forename: P
authors.initials: P
authors.lastname: Gepts
authors.forename: M A
authors.initials: MA
authors.lastname: Pastor-Corrales
mesh_terms: D000483:Alleles; D001947:Breeding; D002874:Chromosome Mapping; D020231:Colletotrichum; D003433:Crosses, Genetic; D018744:DNA, Plant / Q000235:genetics; D060467:Disease Resistance / Q000235:genetics*; D017343:Genes, Plant; D008040:Genetic Linkage; D005819:Genetic Markers; D054884:Host-Pathogen Interactions / Q000235:genetics; D005810:Multigene Family; D027805:Phaseolus / Q000235:genetics* / Q000382:microbiology*; D010935:Plant Diseases / Q000235:genetics* / Q000382:microbiology; D018515:Plant Leaves / Q000235:genetics / Q000382:microbiology; D040641:Quantitative Trait Loci; D016324:Sequence Tagged Sites
chemical_list: D018744:DNA, Plant; D005819:Genetic Markers