title: [Data on liver enzyme and histological changes caused by intermittent clampings of the hepatoduodenal ligament in an experimental model].
abstract: Intermittent compression of the structures of the hepatoduodenal ligament, is often performed during liver surgery. As a result, changes in hepatic blood supply and consequent reperfusion induced tissue damages will develop. Ischemia-reperfusion injury, which occur in local and distant regions, influence outcome of hepatic surgery, and it is in close correlation with the duration of hypoxia during the intervention. In animal model the effect of Baron/Pringle manoeuvre was investigated in terms of changes in liver function tests and histology. The study was carried out on 12 Beagle dogs, clamping of the hepatoduodenal ligament for 3×15 minutes then half an hour reperfusion was performed followed by blood and tissue sampling. Significant histological changes were observed both in the liver as well as the small intestine. In terms of liver function changes, GPT elevation occurred the earliest, GOT and LDH were also increased at the end of the 30 minutes reperfusion. In this animal model, the third 15 minutes compression turned out to be too long. Elevation in GPT levels was the most sensitive marker.
authors.affiliation: Debreceni Egyetem, Orvos- és Egészségtudományi Centrum, Onkológiai Intézet, Sugárterápia Tanszék, 4032 Debrecen, Nagyerdei krt. 98. furkaandi@hotnail.com
authors.forename: Andrea
authors.initials: A
authors.lastname: Furka
authors.forename: Norbert
authors.initials: N
authors.lastname: Németh
authors.forename: Katalin
authors.initials: K
authors.lastname: Pető
authors.forename: Zsolt
authors.initials: Z
authors.lastname: Szentkereszty
authors.forename: László
authors.initials: L
authors.lastname: Tóth
authors.forename: István
authors.initials: I
authors.lastname: Furka
authors.forename: Irén
authors.initials: I
authors.lastname: Mikó
authors.forename: Péter
authors.initials: P
authors.lastname: Sápy
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D003250:Constriction; D004285:Dogs; D004386:Duodenum; D008022:Ligaments / Q000473:pathology; D008099:Liver / Q000098:blood supply / Q000201:enzymology* / Q000473:pathology*; D008102:Liver Circulation; D008111:Liver Function Tests; D023421:Models, Animal; D015427:Reperfusion Injury / Q000097:blood* / Q000201:enzymology; D013997:Time Factors
chemical_list: D015415:Biomarkers; D000410:Alanine Transaminase