title: Coprobacter fastidiosus gen. nov., sp. nov., a novel member of the family Porphyromonadaceae isolated from infant faeces.
abstract: A novel obligately anaerobic, non-spore-forming, rod-shaped, non-motile Gram-reaction-negative bacterium was isolated from infant faeces. The strain, designated NSB1(T), was able to grow on rich media at 30-37 °C, in the presence of up to 2 % (w/v) Oxgall and 2 % (w/v) NaCl. Cells of strain NSB1(T) produced catalase, but not urease and indole. Aesculin was not hydrolysed. The strain was able to utilize d-glucose, lactose, maltose, mannose and raffinose as electron donors. When grown on d-glucose, the main metabolic end products were propionic and acetic acids, with a minor product being succinic acid. The major cellular fatty acids, iso-C15 : 0 and anteiso-C15 : 0, were present at a 1 : 1 molar ratio. The major menaquinone was MK-11. The DNA G+C content was found to be 38.5 mol%. According to 16S rRNA gene sequence analysis strain NSB1(T) is a member of the family Porphyromonadaceae, phylum Bacteroidetes. The closest relatives of the strain were Barnesiella viscericola (88.2 % identity) and Barnesiella intestinihominis (87.4 % identity). On the basis of phenotypic and genotypic properties of strain NSB1(T) we conclude that this strain represent a novel species in a new genus within the family of Porphyromonadaceae for which the name Coprobacter fastidiosus gen. nov., sp. nov. is proposed. The type strain of the species is NSB1(T) ( = DSM 26242(T), = VKM B-2743(T)).
authors.affiliation: Department of Microbiology and Virology, Russian National Research Medical University, Moscow 117997, Russia.
authors.forename: Andrei N
authors.initials: AN
authors.lastname: Shkoporov
authors.affiliation: Department of Microbiology and Virology, Russian National Research Medical University, Moscow 117997, Russia.
authors.forename: Ekaterina V
authors.initials: EV
authors.lastname: Khokhlova
authors.affiliation: Department of Microbiology and Virology, Russian National Research Medical University, Moscow 117997, Russia.
authors.forename: Andrei V
authors.initials: AV
authors.lastname: Chaplin
authors.affiliation: Department of Microbiology and Virology, Russian National Research Medical University, Moscow 117997, Russia.
authors.forename: Lyudmila I
authors.initials: LI
authors.lastname: Kafarskaia
authors.affiliation: Department of Chemistry, Russian National Research Medical University, Moscow 117997, Russia.
authors.forename: Alexei A
authors.initials: AA
authors.lastname: Nikolin
authors.affiliation: Department of Electron Microscopy, Belozersky Institute of Physicochemical Biology, Moscow State University, Moscow 119992, Russia.
authors.forename: Vladimir Yu
authors.initials: VY
authors.lastname: Polyakov
authors.affiliation: Laboratory of Anaerobic Microorganisms, Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino 142290, Russia.
authors.forename: Victoria A
authors.initials: VA
authors.lastname: Shcherbakova
authors.affiliation: Department of Microbiology and Virology, Russian National Research Medical University, Moscow 117997, Russia.
authors.forename: Zoya A
authors.initials: ZA
authors.lastname: Chernaia
authors.affiliation: Department of Microbiology and Virology, Russian National Research Medical University, Moscow 117997, Russia.
authors.forename: Boris A
authors.initials: BA
authors.lastname: Efimov
mesh_terms: D001421:Bacteria, Anaerobic / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D041963:Bacteroidetes / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D005243:Feces / Q000382:microbiology*; D006090:Gram-Negative Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D007223:Infant; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2