title: Molecularly imprinted solid-phase extraction for selective trace analysis of trifluoperazine.
abstract: In this study, a novel sample clean-up technique based on the molecularly imprinted solid-phase extraction procedure is described for the determination of trifluoperazine (TFP) in biological fluids. The water-compatible molecularly imprinted polymers (MIPs) were prepared by using methacrylic acid as functional monomer, ethylene glycol dimethacrylate as cross-linker, chloroform as porogen and TFP as the template molecule. The novel imprinted polymer was used as a solid-phase extraction sorbent for the extraction of TFP from human serum and urine samples. Various parameters affecting the extraction efficiency of the polymer were evaluated. The selectivity of MIPs was evaluated by checking several substances with molecular structures similar to the template. The limits of detection and quantification for TFP in urine samples were 0.06 and 0.2 µg/L, respectively. These limits for TFP in serum samples were 0.15 and 0.4 µg/L, respectively. The recovery values for serum and urine samples were higher than 92 and 93%, respectively.
authors.affiliation: Department of Chemistry, Payame Noor University, Delijan, Iran amohammadattaran@gmail.com mehranjavanbakht@gmail.com.
authors.forename: Abdol Mohammad
authors.initials: AM
authors.lastname: Attaran
authors.affiliation: Department of Chemistry, Payame Noor University, Isfahan, Iran.
authors.forename: Narges
authors.initials: N
authors.lastname: Mohammadi
authors.affiliation: Department of Chemistry, Amirkabir University of Technology, Tehran, Iran amohammadattaran@gmail.com mehranjavanbakht@gmail.com.
authors.forename: Mehran
authors.initials: M
authors.lastname: Javanbakht
authors.affiliation: Food & Drug Laboratory Research Center, Food & Drug Department, Ministry of Health and Medical Education, Tehran, Iran.
authors.forename: Behrouz
authors.initials: B
authors.lastname: Akbari-Adergani
mesh_terms: D000328:Adult; D002725:Chloroform; D005260:Female; D006801:Humans; D057230:Limit of Detection; D008689:Methacrylates; D054802:Molecular Imprinting / Q000379:methods*; D015203:Reproducibility of Results; D052616:Solid Phase Extraction / Q000379:methods*; D014268:Trifluoperazine / Q000032:analysis* / Q000737:chemistry; D055815:Young Adult
chemical_list: D008689:Methacrylates; C008384:methacrylic acid; D014268:Trifluoperazine; C004919:ethylene dimethacrylate; D002725:Chloroform