title: Response to fish specific reproductive hormones and endocrine disrupting chemicals of a Sertoli cell line expressing endogenous receptors from an endemic cyprinid Gnathopogon caerulescens.
abstract: Fish Sertoli cells play a critical role in spermatogenesis by mediating androgen and progestogen signaling. Their hormonal response, however, considerably differ among species. Therefore it would be ideal to use Sertoli cells originated from the fish of interest to investigate the effects of hormones as well as endocrine disrupting chemicals (EDCs). The aim of this study was to investigate the responses to reproductive hormones and EDCs of a Sertoli cell line that we established from an endemic cyprinid Gnathopogon caerulescens. As the Sertoli cell line expressed endogenous androgen and progestogen receptors, we were able to detect hormone responses by transfecting only a reporter vector (pGL4.36) expressing luciferase under the control of the mouse mammary tumor virus-long terminal repeat (MMTV-LTR) promoter into the cell line. Unlike previous reporter gene assays using fish steroid hormone receptors expressed in mammalian cell lines, luciferase activities were induced by the fish specific androgen (11-ketotestosterone) and progestogen (17α,20β-dihydroxy-4-pregnen-3-one), but not by testosterone and progesterone, at physiologically relevant concentrations. Furthermore, we found 4-nonylphenol (NP) but not bisphenol A showed strong anti-androgenic effects, implying that NP may have direct anti-androgenic effects on fish Sertoli cells in vivo. This is the first evidence, to the best of our knowledge, of anti-androgenic effects of NP in a fish Sertoli cell line. In addition, neither NP nor BPA showed anti-progestogenic effects. These results suggest that the Sertoli cell line established from the fish of interest can be a useful in vitro tool for investigating the mechanisms of reproductive hormones and EDCs in the specific fish.
authors.affiliation: Ritsumeikan Global Innovation Research Organization, Ritsumeikan University, Kusatsu, Shiga 525-8577, Japan.
authors.forename: Shogo
authors.initials: S
authors.lastname: Higaki
authors.forename: Yoshie
authors.initials: Y
authors.lastname: Koyama
authors.forename: Manami
authors.initials: M
authors.lastname: Shimada
authors.forename: Yuriko
authors.initials: Y
authors.lastname: Ono
authors.forename: Ikuo
authors.initials: I
authors.lastname: Tooyama
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Fujioka
authors.forename: Noriyoshi
authors.initials: N
authors.lastname: Sakai
authors.forename: Toshitaka
authors.initials: T
authors.lastname: Ikeuchi
authors.forename: Tatsuyuki
authors.initials: T
authors.lastname: Takada
mesh_terms: D000818:Animals; D001559:Benzhydryl Compounds / Q000633:toxicity; D002460:Cell Line; D003530:Cyprinidae / Q000235:genetics / Q000378:metabolism; D052244:Endocrine Disruptors / Q000633:toxicity*; D017930:Genes, Reporter / Q000235:genetics; D008297:Male; D010636:Phenols / Q000633:toxicity; D012098:Reproduction / Q000502:physiology*; D012708:Sertoli Cells / Q000187:drug effects* / Q000378:metabolism*; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D052244:Endocrine Disruptors; D010636:Phenols; C041594:4-nonylphenol; C006780:bisphenol A; D000098826:Bisphenol A Compounds
keywords: Endemic cyprinid; Endocrine disruptor; Reporter gene; Reproductive steroid; Sertoli cell