title: Osteopontin mediating cyclosporine A induced epithelial-to-mesenchymal transition on rat renal tubular epithelial cells.
abstract: Osteopontin (OPN) is highly correlated with cyclosporine A (CsA) nephrotoxicity. As epithelial-to-mesenchymal transition (EMT) of renal tubular epithelial cells plays an important role in CsA nephropathy, we investigated whether OPN mediated EMT of renal tubular epithelial cells upon CsA stimulation. OPN knockdown suppresses CsA induced EMT on NRK52E cells, and it also attenuates downregulation of E-cadherin and upregulation of α-smooth muscle actin (α-SMA) and fibronectin (FN) that are induced by CsA. OPN alone can induce EMT on NRK52E cells, which also results in upregulation of TGF-β1. Thus, OPN is a causative factor in mediating CsA induced EMT on NRK52E cells.
authors.affiliation: Department of Urology, The First People's Hospital Affiliated to Shanghai Jiao Tong University, Shanghai First People's Hospital, 100 Haining Road, Shanghai, 200080, China.
authors.forename: Dongliang
authors.initials: D
authors.lastname: Xu
authors.forename: Xia
authors.initials: X
authors.lastname: Chen
authors.forename: Zhonglei
authors.initials: Z
authors.lastname: Deng
authors.forename: Ruoyun
authors.initials: R
authors.lastname: Tan
authors.forename: Chao
authors.initials: C
authors.lastname: Liu
authors.forename: Pei
authors.initials: P
authors.lastname: Lu
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: Min
authors.initials: M
authors.lastname: Gu
mesh_terms: D000199:Actins / Q000378:metabolism; D000818:Animals; D015820:Cadherins / Q000378:metabolism; D002460:Cell Line; D016572:Cyclosporine / Q000494:pharmacology*; D015536:Down-Regulation / Q000187:drug effects; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism; D058750:Epithelial-Mesenchymal Transition / Q000187:drug effects*; D005353:Fibronectins / Q000378:metabolism; D007166:Immunosuppressive Agents / Q000494:pharmacology; D007684:Kidney Tubules / Q000166:cytology; D053495:Osteopontin / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D053773:Transforming Growth Factor beta1 / Q000378:metabolism; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D000199:Actins; D015820:Cadherins; D016572:Cyclosporine; D005353:Fibronectins; D007166:Immunosuppressive Agents; D053495:Osteopontin; D034741:RNA, Small Interfering; D053773:Transforming Growth Factor beta1; C498862:Acta2 protein, rat
keywords: EMT; cyclosporine A; osteopontin