title: Comparative analysis of 16S RNA nucleotide sequences of Anaplasma phagocytophilum detected in the blood of horses from various parts of Europe.
abstract: The aim of this study was to conduct a comparative analysis of the 16S rRNA gene fragment nucleotide sequences for Anaplasma phagocytophilum strains detected in the blood of horses from various parts of Europe. The study comprised 234 horses that had had contact with ticks. Using PCR, the genetic material of A. phagocytophilum was identified in the blood of 42 animals. The sequences of the 16S RNA gene amplicons that were obtained from our A. phagocytophilum isolates had 100 % similarity with each other and 96.4-100 % similarity with Anaplasma spp. sequences selected from those available in GenBank. Nucleotide substitutions at positions 248 and 249 were demonstrated in all the 16S RNA gene sequences of Anaplasma obtained in our study. This may indicate the emergence of a new rickettsial genotype that is the cause of equine granulocytic anaplasmosis in southern and eastern Europe. These results add new information on the epidemiology and genetic diversity of A. phagocytophilum detected in the blood of horses from southern and eastern Europe.
authors.affiliation: Department of Epizootiology and Infectious Diseases, Faculty of Veterinary Medicine, University of Life Sciences Lublin, 30 Głęboka St 20-612 Lublin, Poland.
authors.forename: Beata
authors.initials: B
authors.lastname: Dzięgiel
authors.affiliation: Department of Epizootiology and Infectious Diseases, Faculty of Veterinary Medicine, University of Life Sciences Lublin, 30 Głęboka St 20-612 Lublin, Poland.
authors.forename: Lukasz
authors.initials: L
authors.lastname: Adaszek
authors.affiliation: Department of Vitreoretinal Surgery, Medical University of Lublin, Chmielna 1, 20-079 Lublin, Poland.
authors.forename: Mateusz
authors.initials: M
authors.lastname: Winiarczyk
authors.affiliation: Departamento de Sanidad Animal, Facultad de Veterinaria, UCO Campus, Universitarios de Rabanales, 14071 Córdoba, Spain.
authors.forename: Ignacio
authors.initials: I
authors.lastname: García-Bocanegra
authors.affiliation: Departamento de Sanidad Animal, Facultad de Veterinaria, UCO Campus, Universitarios de Rabanales, 14071 Córdoba, Spain.
authors.forename: Alfonso
authors.initials: A
authors.lastname: Carbonero
authors.affiliation: Laboratory of Radiology and Ultrasonography, Department and Clinic of Animal Surgery, University of Life Science in Lublin, Gleboka 30 St, 20-612 Lublin, Poland.
authors.forename: Piotr
authors.initials: P
authors.lastname: Dębiak
authors.affiliation: Department of Epizootiology and Infectious Diseases, Faculty of Veterinary Medicine, University of Life Sciences Lublin, 30 Głęboka St 20-612 Lublin, Poland.
authors.forename: Stanisław
authors.initials: S
authors.lastname: Winiarczyk
mesh_terms: D041081:Anaplasma phagocytophilum / Q000235:genetics* / Q000302:isolation & purification*; D000818:Animals; D001483:Base Sequence / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics; D016873:Ehrlichiosis / Q000382:microbiology*; D005060:Europe; D005838:Genotype; D006736:Horses; D009711:Nucleotides / Q000235:genetics; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D013987:Ticks / Q000382:microbiology
chemical_list: D004269:DNA, Bacterial; D009711:Nucleotides; D012336:RNA, Ribosomal, 16S