title: Electrochemical determination of glutathione in plasma at carbon nanotubes based screen printed electrodes.
abstract: Glutathione (GSH) is a major endogenous antioxidant highly active in human tissues and plays a key role in controlling cellular thiol redox system, maintaining the immune and detoxification system. The determination of GSH levels in tissue is important to estimate endogenous defenses against oxidative stress. In our study, the multi-walled carbon nanotube modified screen-printed electrodes (MWCNT-SPEs) were used to determine the levels of GSH in trichloroacetic acid (TCA)-treated or untreated samples of rat plasma. It was found that the deproteinization of samples with TCA improved the electrochemical detection of GSH particularly in plasma. The oxidation of GSH was measured by using differential pulse voltammetry (DPV) method in combination with MWCNT-SPE (n=3), and the detection limit of GSH was found to be 0.47 µM (S/N=3). The GSH levels in plasma samples were also measured spectrophotometrically in order to compare the effectiveness of electrochemical method and we obtained a high correlation between the two methods (R(2)=0.976).
authors.affiliation: Department of Analytical Chemistry, Faculty of Pharmacy, Ege University, 35100 Bornova, Izmir, Turkey. arzum.erdem@ege.edu.tr.
authors.forename: Ezgi
authors.initials: E
authors.lastname: Turunc
authors.forename: Hakan
authors.initials: H
authors.lastname: Karadeniz
authors.forename: Guliz
authors.initials: G
authors.lastname: Armagan
authors.forename: Arzum
authors.initials: A
authors.lastname: Erdem
authors.forename: Ayfer
authors.initials: A
authors.lastname: Yalcin
mesh_terms: D000818:Animals; D055664:Electrochemical Techniques / Q000295:instrumentation*; D004566:Electrodes; D004867:Equipment Design; D005978:Glutathione / Q000097:blood*; D057230:Limit of Detection; D037742:Nanotubes, Carbon / Q000737:chemistry*; D051381:Rats
chemical_list: D037742:Nanotubes, Carbon; D005978:Glutathione