title: DNA fragmentation is higher in spermatozoa with chromosomally unbalanced content in men with a structural chromosomal rearrangement.
abstract: It has been previously shown that men with chromosomal structural abnormality had a higher rate of sperm DNA fragmentation. We studied 11 male carriers of a chromosomal structural abnormality (seven with a balanced reciprocal translocation, three with a Robertsonian translocation, one with a pericentric inversion) to determine whether spermatozoa with unbalanced chromosomes were more likely to have fragmented DNA. A sequential method combining analysis of DNA fragmentation using the TUNEL assay followed by analysis of meiotic segregation by fluorescent in situ hybridization was performed on the same spermatozoa. A statistically significant higher number of spermatozoa with unbalanced chromosomal content were found to have fragmented DNA for each man. The rate of spermatozoa with DNA fragmentation was higher than the rate of those without fragmented DNA in particular modes of segregation. Our findings provide a better understanding of the mechanisms involved in male infertility ascribable to chromosomal structural abnormality.
authors.affiliation: Laboratoire d'Histologie, Embryologie et Cytogénétique, Faculté de Médecine et des Sciences de la Santé, Université de Bretagne Occidentale, Brest, France.
authors.forename: A
authors.initials: A
authors.lastname: Perrin
authors.forename: M H
authors.initials: MH
authors.lastname: Nguyen
authors.forename: L
authors.initials: L
authors.lastname: Bujan
authors.forename: F
authors.initials: F
authors.lastname: Vialard
authors.forename: V
authors.initials: V
authors.lastname: Amice
authors.forename: N
authors.initials: N
authors.lastname: Guéganic
authors.forename: N
authors.initials: N
authors.lastname: Douet-Guilbert
authors.forename: M
authors.initials: M
authors.lastname: De Braekeleer
authors.forename: F
authors.initials: F
authors.lastname: Morel
mesh_terms: D020090:Chromosome Segregation; D053938:DNA Fragmentation; D005298:Fertility / Q000235:genetics*; D015321:Gene Rearrangement; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D020287:In Situ Nick-End Labeling; D007248:Infertility, Male / Q000235:genetics* / Q000473:pathology / Q000503:physiopathology; D008297:Male; D008540:Meiosis; D055101:Semen Analysis / Q000379:methods; D013094:Spermatozoa / Q000473:pathology*; D014178:Translocation, Genetic