title: Adenovirus encoding BMP-7 immobilized on titanium surface exhibits local delivery ability and regulates osteoblast differentiation in vitro.
abstract: OBJECTIVE
  The local delivery of growth factors such as bone morphogenetic protein-7 (BMP-7) into the tissues around dental implants may improve their osseointegration. We have designed a new method of attaching BMP-7 to a titanium surface and assessed both the retention of the BMP-7 and its effect on osteoblast differentiation.
  
  
  METHODS
  Adenoviral vector expressing BMP-7 was attached to dental titanium discs by hexon-specific antibodies in a type I collagen-avidin gel. FITC-labelled secondary antibody was used to measure the continuing adherence of the coating after repeated rinsing. Osteoblasts were harvested and seeded on the titanium discs. Gene transduction efficiency and targeting ability were assessed after 24h. Surface morphology was observed by SEM. Cell proliferation and alkaline phosphatase (ALP) activities were measured.
  
  
  RESULTS
  The anti-adenohexon antibody adhered strongly to the collagen-avidin gels. BMP-7 gene expression was localized precisely to cells growing on the gels bound by the hexon-specific antibody. Osteoblasts on the titanium containing Ad-BMP-7 had a higher ALP activity than those without Ad-BMP-7.
  
  
  CONCLUSIONS
  This study describes a novel technique for the precise attachment of BMP-7 to titanium surfaces. The process may enhance the osseointegration of dental implants.
authors.affiliation: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, #14 Sec. 3 Renmin Road South, Chengdu, PR China.
authors.forename: Shenyuan
authors.initials: S
authors.lastname: Chen
authors.forename: Jiahua
authors.initials: J
authors.lastname: Yang
authors.forename: Hang
authors.initials: H
authors.lastname: Wang
authors.forename: Yonglie
authors.initials: Y
authors.lastname: Chao
authors.forename: Chaoliang
authors.initials: C
authors.lastname: Zhang
authors.forename: Jiefei
authors.initials: J
authors.lastname: Shen
authors.forename: Ping
authors.initials: P
authors.lastname: Zhang
mesh_terms: D000256:Adenoviridae / Q000235:genetics* / Q000378:metabolism; D000469:Alkaline Phosphatase / Q000235:genetics* / Q000378:metabolism; D000704:Analysis of Variance; D000818:Animals; D055419:Bone Morphogenetic Protein 7 / Q000494:pharmacology*; D002417:Cattle; D002448:Cell Adhesion / Q000235:genetics; D002454:Cell Differentiation; D002478:Cells, Cultured; D003094:Collagen / Q000494:pharmacology; D015921:Dental Implants; D004337:Drug Carriers / Q000494:pharmacology*; D018014:Gene Transfer Techniques; D008855:Microscopy, Electron, Scanning; D016348:Osseointegration / Q000187:drug effects*; D010006:Osteoblasts / Q000187:drug effects*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013499:Surface Properties; D014025:Titanium
chemical_list: D055419:Bone Morphogenetic Protein 7; D015921:Dental Implants; D004337:Drug Carriers; D003094:Collagen; D014025:Titanium; D000469:Alkaline Phosphatase
keywords: Adenoviral vector; Bone morphogenetic protein-7; Gene delivery; Osteoblast