title: Organic acids on the growth, anatomical structure, biochemical parameters and heavy metal accumulation of Iris lactea var. chinensis seedling growing in Pb mine tailings.
abstract: The effect of citric acid (CA) and ethylene diamine tetraacetic acid (EDTA) on the growth, anatomical structure, physiological responses and lead (Pb) accumulation of Iris lactea var. chinensis seedling growing in Pb mine tailings for 30 days were studied. Results showed that the dry weights (DW) of roots decreased significantly under both levels of CA. The DWs of leaves and roots treated with 2 mmol/kg EDTA decreased significantly and were 23 and 54 %, respectively, lower than those of the control. The tolerant indexes of I. lactea var. chinensis under all treatments of organic acids were lower than control. The root tip anatomical structure was little affected under the treatments of 2 mmol/kg CA and 2 mmol/kg EDTA compared with control. However, the formation of photosynthesizing cells was inhibited by the treatment of 2 mmol/kg EDTA. The concentrations of chlorophyll a, chlorophyll b and total carotenoids in the leaves treated with 2 mmol/kg EDTA significantly decreased. Higher CA level and lower EDTA level could trigger the synthesis of ascorbic acid and higher level of EDTA could trigger the synthesis of glutathione. CA and EDTA could promote Pb accumulation of I. lactea var. chinensis and Pb concentration in the leaves and roots at 2 mmol/kg EDTA treatment increased significantly and reached to 160.44 and 936.08 μg/g DW, respectively, and 1.8 and 1.6 times higher than those of the control. The results indicated that I. lactea var. chinensis could be used to remediate Pb tailing and the role of EDTA in promoting Pb accumulation was better than CA did.
authors.affiliation: Experimental Teaching Center of Ecological Environment of Jiangxi Province, Department of Landscape Architecture, Jiangxi University of Finance and Economics, Nanchang, 330032, China. hyl1957@163.com
authors.forename: Yu-Lin
authors.initials: YL
authors.lastname: Han
authors.forename: Su-Zhen
authors.initials: SZ
authors.lastname: Huang
authors.forename: Hai-Yan
authors.initials: HY
authors.lastname: Yuan
authors.forename: Jiu-Zhou
authors.initials: JZ
authors.lastname: Zhao
authors.forename: Ji-Guang
authors.initials: JG
authors.lastname: Gu
mesh_terms: D002734:Chlorophyll / Q000031:analogs & derivatives / Q000032:analysis; D000077194:Chlorophyll A; D019343:Citric Acid / Q000494:pharmacology*; D004492:Edetic Acid / Q000494:pharmacology*; D031323:Iris Plant / Q000187:drug effects* / Q000254:growth & development; D007854:Lead / Q000633:toxicity*; D019216:Metals, Heavy / Q000633:toxicity*; D008906:Mining; D018515:Plant Leaves / Q000187:drug effects / Q000254:growth & development; D018517:Plant Roots / Q000187:drug effects / Q000254:growth & development; D036226:Seedlings / Q000187:drug effects* / Q000254:growth & development; D012989:Soil Pollutants / Q000633:toxicity
chemical_list: D019216:Metals, Heavy; D012989:Soil Pollutants; D002734:Chlorophyll; C085815:chlorophyll a'; D019343:Citric Acid; D007854:Lead; D004492:Edetic Acid; D000077194:Chlorophyll A