title: A simple and innovative method for species identification of phytoplankton cells on minute quantities of DNA.
abstract: Dinoflagellates belonging to the genus Alexandrium are often involved in harmful algal blooms. Their ecological exploration is thus essential to increase our knowledge on these toxic events. Yet, population genetic studies, taxonomic identification and environmental monitoring are hampered by major constraints: the necessity to establish monoclonal cultures from environmental samples and the sensitivity of available molecular tools. The present work describes a very simple and sensitive method for extraction and amplification of DNA at the infra-single-cell level. Its on-slide format allows for easy visual control of both quality and quantity of the templates. Combined with a semi-multiplex PCR protocol designed on the 18S-28S rDNA-ITS region of Alexandrium catenella and Alexandrium tamarense, this procedure allowed the identification and discrimination of these species from both monoclonal cultures and natural samples.
authors.affiliation: Université Montpellier II, CNRS, Ifremer, IRD, UMR 5119 Ecosystèmes Lagunaires, cc093, place E. Bataillon, 34095 Montpellier, France. IRD, UR175 CAVIAR, 361 Rue Jean-François Breton, BP5095, 34196 Montpellier cedex 05, France.
authors.forename: Estelle
authors.initials: E
authors.lastname: Masseret
authors.forename: Marjorie
authors.initials: M
authors.lastname: Enquebecq
authors.forename: Mohamed
authors.initials: M
authors.lastname: Laabir
authors.forename: Benjamin
authors.initials: B
authors.lastname: Genovesi
authors.forename: André
authors.initials: A
authors.lastname: Vaquer
authors.forename: Jean-Christophe
authors.initials: JC
authors.lastname: Avarre