title: Solid-phase synthesis of molecularly imprinted nanoparticles for protein recognition.
abstract: A solid-phase synthesis approach to prepare molecularly imprinted polymer nanoparticles (MIP-NPs) specific for trypsin is described. The protein is immobilized on a solid support upon which thermoresponsive MIP-NPs are synthesized. The MIP-NPs are released by a simple temperature change, resulting in synthetic antibody mimics exhibiting high specificity and selectivity for trypsin.
authors.affiliation: Compiègne University of Technology, CNRS Enzyme and Cell Engineering Laboratory, Rue Roger Couttolenc, CS 60319, 60203 Compiègne Cedex, France.
authors.forename: Serena
authors.initials: S
authors.lastname: Ambrosini
authors.forename: Selim
authors.initials: S
authors.lastname: Beyazit
authors.forename: Karsten
authors.initials: K
authors.lastname: Haupt
authors.forename: Bernadette
authors.initials: B
authors.lastname: Tse Sum Bui
mesh_terms: D000327:Adsorption; D054802:Molecular Imprinting / Q000379:methods*; D053758:Nanoparticles / Q000737:chemistry*; D060327:Solid-Phase Synthesis Techniques / Q000379:methods*; D014357:Trypsin / Q000032:analysis*
chemical_list: D014357:Trypsin