title: Discontinuous nanoporous membranes reduce non-specific fouling for immunoaffinity cell capture.
abstract: The microfluidic isolation of target cells using adhesion-based surface capture has been widely explored for biology and medicine. However, high-throughput processing can be challenging due to interfacial limitations such as transport, reaction, and non-specific fouling. Here, it is shown that antibody-functionalized capture surfaces with discontinuous permeability enable efficient target cell capture at high flow rates by decreasing fouling. Experimental characterization and theoretical modeling reveal that "wall effects" affect cell-surface interactions and promote excess surface accumulation. These issues are partially circumvented by reducing the transport and deposition of cells near the channel walls. Optimized microfluidic devices can be operated at higher cell concentrations with significant improvements in throughput.
authors.affiliation: BioMEMS Resource Center, Center for Engineering in Medicine and Department of Surgery, Massachusetts General Hospital and Harvard Medical School, 114 16th Street, Charlestown, MA 02129, USA; Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
authors.forename: Sukant
authors.initials: S
authors.lastname: Mittal
authors.forename: Ian Y
authors.initials: IY
authors.lastname: Wong
authors.forename: Ahmet Ali
authors.initials: AA
authors.lastname: Yanik
authors.forename: William M
authors.initials: WM
authors.lastname: Deen
authors.forename: Mehmet
authors.initials: M
authors.lastname: Toner
mesh_terms: D000327:Adsorption; D045744:Cell Line, Tumor; D004867:Equipment Design; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D007962:Leukocytes / Q000166:cytology; D008297:Male; D046210:Microfluidic Analytical Techniques; D044085:Microfluidics / Q000295:instrumentation*; D058608:Nanopores; D036103:Nanotechnology; D010316:Particle Size; D010539:Permeability; D012825:Silicon / Q000737:chemistry; D013499:Surface Properties
chemical_list: D012825:Silicon
keywords: biosensors; cell capture; microfluidics; nanoporous membranes; non-specific adsorption