title: Biological evaluation of endophytic fungus, Chaetomium globosum JN711454, as potential candidate for improving drug discovery.
abstract: The main objective of this research work focused on investigating the biological and chemical aspects of endophytic fungus Chaetomium globosum, for pharmaceutical purposes to improve the drug discovery process. The endophytic C. globosum was isolated from healthy leaves of Egyptian medicinal plant Adiantum capillus-veneris collected from Saint Katherine Protectorate, Sinai, Egypt. The identification of C. globosum was on the basis of classical and molecular taxonomy. Gene encoding for 18S rRNA was partially sequenced, submitted to the GenBank and got the accession number JN711454, to resolve the phylogenetic relations with fungal ancestor using phylogenetic tree. To explore the biosynthetic power of endophytic C. globosum JN711454, the fungus was cultivated over five different media, oatmeal, rice, yeast malt glucose, potato dextrose agar (PDA) and Czapek's dox media, for 3 weeks at 30 °C, followed by extraction with different solvents, ethyl acetate (EA), and methanol. The ethyl acetate extract of C. globosum cultivated on PDA medium was the most potent extract. It showed strong antioxidant activity with EC50 11.5 μg/ml, potent anticancer activity with 55 % toxicity toward HepG-2 cells at 100 μg/ml and 66 % cytotoxicity to FGC4 cells at 250 μg/ml, promising butyrylcholinesterase inhibitory activities (>85 %), and moderate antimicrobial and stopped the attachment of HSV-2 virus to VERO cells. The metabolomic profiling of PDA-EA extract using LC-MS revealed the presence of several metabolites to which the observed bioactivities could be attributed. Here we report for the first time inhibitory activity of endophytic C. globosum JN711454 secondary metabolites to butyrylcholinesterase, one of neuro hydrolase enzymes that play a major role in development of Alzheimer's disease.
authors.affiliation: Department of Chemistry of Natural and Microbial Products, National Research Center, Dokki, Cairo, 12622, Egypt.
authors.forename: Khaled A
authors.initials: KA
authors.lastname: Selim
authors.forename: Ahmed A
authors.initials: AA
authors.lastname: El-Beih
authors.forename: Tahany M
authors.initials: TM
authors.lastname: Abdel-Rahman
authors.forename: Ahmed I
authors.initials: AI
authors.lastname: El-Diwany
mesh_terms: D035681:Adiantum / Q000382:microbiology; D000818:Animals; D000890:Anti-Infective Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D001419:Bacteria / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002597:Chaetomium / Q000145:classification / Q000187:drug effects / Q000378:metabolism*; D002522:Chlorocebus aethiops; D055808:Drug Discovery; D004353:Drug Evaluation, Preclinical; D005658:Fungi / Q000187:drug effects; D056945:Hep G2 Cells; D006801:Humans; D010802:Phylogeny; D018515:Plant Leaves / Q000382:microbiology; D012328:RNA Viruses / Q000187:drug effects; D012337:RNA, Ribosomal, 18S / Q000737:chemistry / Q000235:genetics; D014709:Vero Cells
chemical_list: D000890:Anti-Infective Agents; D000970:Antineoplastic Agents; D000975:Antioxidants; D012337:RNA, Ribosomal, 18S