title: Association of Myosin Va and Schwann cells-derived RNA in mammal myelinated axons, analyzed by immunocytochemistry and confocal FRET microscopy.
abstract: Evidence from multiple sources supports the hypothesis that Schwann cells in the peripheral nervous system transfer messenger RNA and ribosomes to the axons they ensheath. Several technical and methodological difficulties exist for investigators to unravel this process in myelinated axons - a complex two-cell unit. We present an experimental design to demonstrate that newly synthesized RNA is transferred from Schwann cells to axons in association with Myosin Va. The use of quantitative confocal FRET microscopy to track newly-synthesized RNA and determine the molecular association with Myosin Va, is described in detail.
authors.affiliation: Departamento de Proteínas y Acidos Nucleicos, Instituto de Investigaciones Biológicas Clemente Estable, Av. Italia 3318, Montevideo, CP 11600, Uruguay. Electronic address: lcanclini@fcien.edu.uy.
authors.forename: Lucía
authors.initials: L
authors.lastname: Canclini
authors.affiliation: Department of Biology, University of Virginia, P.O. Box 400328, Charlottesville, VA 22904-4328, USA. Electronic address: hw5m@virginia.edu.
authors.forename: Horst
authors.initials: H
authors.lastname: Wallrabe
authors.affiliation: Departamento de Proteínas y Acidos Nucleicos, Instituto de Investigaciones Biológicas Clemente Estable, Av. Italia 3318, Montevideo, CP 11600, Uruguay. Electronic address: apdipaolo@gmail.com.
authors.forename: Andrés
authors.initials: A
authors.lastname: Di Paolo
authors.affiliation: Department of Biology, University of Virginia, P.O. Box 400328, Charlottesville, VA 22904-4328, USA; Sección Bioquímica, Facultad de Ciencias, Universidad de la República, Iguá 4225, Montevideo, CP 11400, Uruguay. Electronic address: kun@iibce.edu.uy.
authors.forename: Alejandra
authors.initials: A
authors.lastname: Kun
authors.affiliation: Departamento de Proteínas y Acidos Nucleicos, Instituto de Investigaciones Biológicas Clemente Estable, Av. Italia 3318, Montevideo, CP 11600, Uruguay; Area Biofísica, Departamento de Bioquímica, Biología Celular y Molecular, Facultad de Veterinaria, Alberto Lasplaces 1550, Montevideo, CP 11600, Uruguay. Electronic address: aldo@iibce.edu.uy.
authors.forename: Aldo
authors.initials: A
authors.lastname: Calliari
authors.affiliation: Departamento de Genética, Instituto de Investigaciones Biológicas Clemente Estable, Av Italia 3318, Montevideo, CP 11600, Uruguay; Departamento de Biología Celular, Facultad de Ciencias, Universidad de la República, Iguá 4225, Montevideo, CP 11400, Uruguay. Electronic address: coya@iibce.edu.uy.
authors.forename: José Roberto
authors.initials: JR
authors.lastname: Sotelo-Silveira
authors.affiliation: Departamento de Proteínas y Acidos Nucleicos, Instituto de Investigaciones Biológicas Clemente Estable, Av. Italia 3318, Montevideo, CP 11600, Uruguay. Electronic address: sotelotalibo@gmail.com.
authors.forename: José Roberto
authors.initials: JR
authors.lastname: Sotelo
mesh_terms: D000818:Animals; D001369:Axons / Q000378:metabolism*; D031541:Fluorescence Resonance Energy Transfer; D007150:Immunohistochemistry; D018613:Microscopy, Confocal; D018995:Myosin Heavy Chains / Q000378:metabolism*; D024701:Myosin Type V / Q000378:metabolism*; D010525:Peripheral Nerves / Q000166:cytology / Q000378:metabolism; D034443:RNA Transport; D012333:RNA, Messenger / Q000378:metabolism*; D011901:Ranvier's Nodes / Q000378:metabolism*; D051381:Rats; D012583:Schwann Cells / Q000378:metabolism
chemical_list: C513081:Myo5a protein, rat; D012333:RNA, Messenger; D024701:Myosin Type V; D018995:Myosin Heavy Chains
keywords: Axon; Myosin Va; Quantitative FRET microscopy; RNA cell-to-cell transfer; RNA transport; Schwann cell