title: A serine-substituted P450 catalyzes highly efficient carbene transfer to olefins in vivo.
abstract: Whole-cell catalysts for non-natural chemical reactions will open new routes to sustainable production of chemicals. We designed a cytochrome 'P411' with unique serine-heme ligation that catalyzes efficient and selective olefin cyclopropanation in intact Escherichia coli cells. The mutation C400S in cytochrome P450(BM3) gives a signature ferrous CO Soret peak at 411 nm, abolishes monooxygenation activity, raises the resting-state Fe(III)-to-Fe(II) reduction potential and substantially improves NAD(P)H-driven activity.
authors.affiliation: Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, California, USA.
authors.forename: Pedro S
authors.initials: PS
authors.lastname: Coelho
authors.forename: Z Jane
authors.initials: ZJ
authors.lastname: Wang
authors.forename: Maraia E
authors.initials: ME
authors.lastname: Ener
authors.forename: Stefanie A
authors.initials: SA
authors.lastname: Baril
authors.forename: Arvind
authors.initials: A
authors.lastname: Kannan
authors.forename: Frances H
authors.initials: FH
authors.lastname: Arnold
authors.forename: Eric M
authors.initials: EM
authors.lastname: Brustad
mesh_terms: D000475:Alkenes / Q000737:chemistry / Q000378:metabolism*; D055162:Biocatalysis; D003577:Cytochrome P-450 Enzyme System / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D004926:Escherichia coli / Q000737:chemistry / Q000166:cytology / Q000378:metabolism; D008697:Methane / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D009154:Mutation; D012694:Serine / Q000737:chemistry / Q000378:metabolism*
chemical_list: D000475:Alkenes; C030011:carbene; D012694:Serine; D003577:Cytochrome P-450 Enzyme System; D008697:Methane