title: [Mutation-specific antibodies for detection of epidermal growth factor receptor mutations in patients with lung adenocarcinoma].
abstract: OBJECTIVE
  To examine the sensitivity and specificity of the two epidermal growth factor receptor hotspot mutation antibodies-delE746-A750 and L858R in lung adenocarcinoma, and to discuss the value of the two antibodies for detecting these two "classic mutations" by immunohistochemistry.
  
  
  METHODS
  With Q-scoring assessment and ROC curve analysis,immunohistochemistry was used to screen a panel of 309 paraffin-embedded non-small cell lung cancer(NSCLC) tumor samples, of which EGFR gene mutational status was previously documented by PCR-DNA sequencing.
  
  
  RESULTS
  Three hundred and nine NSCLC tumor samples conclude 92 NSCLC cases with 19delE746-A750 and 110 with 21L858R. Using DelEGFR antibody to detect 19delE746-A750 demonstrated sensitivity and specificity of 84.8% (78/92) and 77.6% (83/107), respectively. Choosing the best cut-off value of 85.00, it achieved the maximum area under the curve (AUC:0.885). L858R antibody to detect 21L858R showed sensitivity and specificity of 94.5% (104/110) and 62.5% (55/88), respectively. Choosing the best cut-off value of 97.50, it achieved the maximum area under the curve (AUC:0.924).
  
  
  CONCLUSIONS
  By using Q-scoring assessment and ROC curve analysis, the two antibodies (delEGFR and L858R) can been used to identify EGFR gene mutations present in lung adenocarcinomas.
authors.affiliation: Department of Pathology, Shanghai Jiaotong University, Shanghai, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Zhang
authors.forename: Jie
authors.initials: J
authors.lastname: Zhang
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D000077192:Adenocarcinoma of Lung; D000368:Aged; D000911:Antibodies, Monoclonal; D019540:Area Under Curve; D066246:ErbB Receptors / Q000235:genetics*; D005091:Exons; D005260:Female; D017353:Gene Deletion; D006801:Humans; D007150:Immunohistochemistry; D008175:Lung Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D008297:Male; D016612:Paraffin Embedding; D017354:Point Mutation; D012680:Sensitivity and Specificity
chemical_list: D000911:Antibodies, Monoclonal; C512478:EGFR protein, human; D066246:ErbB Receptors