title: Isolation and characterization of multipotent cells from human fetal dermis.
abstract: We report that cells from human fetal dermis, termed here multipotent fetal dermal cells, can be isolated with high efficiency by using a nonenzymatic, cell outgrowth method. The resulting cell population was consistent with the definition of mesenchymal stromal cells by the International Society for Cellular Therapy. As multipotent fetal dermal cells proliferate extensively, with no loss of multilineage differentiation potential up to passage 25, they may be an ideal source for cell therapy to repair damaged tissues and organs. Multipotent fetal dermal cells were not recognized as targets by T lymphocytes in vitro, thus supporting their feasibility for allogenic transplantation. Moreover, the expansion protocol did not affect the normal phenotype and karyotype of cells. When compared with adult dermal cells, fetal cells displayed several advantages, including a greater cellular yield after isolation, the ability to proliferate longer, and the retention of differentiation potential. Interestingly, multipotent fetal dermal cells expressed the pluripotency marker SSEA4 (90.56 ± 3.15% fetal vs. 10.5 ± 8.5% adult) and coexpressed mesenchymal and epithelial markers (>80% CD90(+)/CK18(+) cells), coexpression lacking in the adult counterparts isolated under the same conditions. Multipotent fetal dermal cells were able to form capillary structures, as well as differentiate into a simple epithelium in vitro, indicating skin regeneration capabilities.
authors.affiliation: Fondazione Ri.MED, Regenerative Medicine and Biomedical Technologies Unit, Department of Laboratory Medicine and Advanced Biotechnologies, ISMETT, Palermo, Italy.
authors.forename: Cinzia Maria
authors.initials: CM
authors.lastname: Chinnici
authors.forename: Giandomenico
authors.initials: G
authors.lastname: Amico
authors.forename: Marcello
authors.initials: M
authors.lastname: Monti
authors.forename: Stefania
authors.initials: S
authors.lastname: Motta
authors.forename: Rosario
authors.initials: R
authors.lastname: Casalone
authors.forename: Sergio Li
authors.initials: SL
authors.lastname: Petri
authors.forename: Marco
authors.initials: M
authors.lastname: Spada
authors.forename: Bruno
authors.initials: B
authors.lastname: Gridelli
authors.forename: Pier Giulio
authors.initials: PG
authors.lastname: Conaldi
mesh_terms: D002454:Cell Differentiation / Q000502:physiology; D020405:Dermis / Q000166:cytology* / Q000196:embryology; D005260:Female; D053686:Fetal Stem Cells / Q000166:cytology*; D006801:Humans; D008297:Male; D039902:Multipotent Stem Cells / Q000166:cytology*