title: Organization of F-actin by Fesselin (avian smooth muscle synaptopodin 2).
abstract: Fesselin or avian synaptopodin 2 is a member of the synaptopodin family of actin binding proteins. Fesselin promotes G-actin polymerization and the formation of large actin complexes that can be collected by low-speed centrifugation. Because of the potential role of fesselin in some cancers and its effects on actin, we further investigated the effect of fesselin on actin. Fesselin initiated actin polymerization under a variety of conditions, including the virtual absence of salt. Actin filaments formed at low salt concentrations in the presence of fesselin were similar to filaments polymerized in the presence of 100 mM KCl. In both cases, the filaments were long and straight with a common orientation. Highly ordered actin bundles formed with increasing times of incubation. Blockers of actin growth at the barbed end (cytochalasin D and CapZ) did not prevent fesselin from polymerizing actin. Low concentrations of fesselin increased the critical concentration of actin. Both observations are consistent with preferential growth at the pointed end of actin filaments. These results indicate a role of fesselin in organizing cellular actin. These and other results indicate that fesselin is part of a cellular actin organizing center.
authors.affiliation: Institute of Vegetative Physiology, University of Cologne , Robert Koch Strasse 39, D-50931 Cologne, Germany.
authors.forename: Mechthild M
authors.initials: MM
authors.lastname: Schroeter
authors.forename: Albina
authors.initials: A
authors.lastname: Orlova
authors.forename: Edward H
authors.initials: EH
authors.lastname: Egelman
authors.forename: Brent
authors.initials: B
authors.lastname: Beall
authors.forename: Joseph M
authors.initials: JM
authors.lastname: Chalovich
mesh_terms: D000199:Actins / Q000737:chemistry*; D000818:Animals; D001717:Birds; D008565:Membrane Proteins / Q000737:chemistry*; D008840:Microfilament Proteins / Q000737:chemistry*; D008854:Microscopy, Electron; D011487:Protein Conformation
chemical_list: D000199:Actins; D008565:Membrane Proteins; D008840:Microfilament Proteins; C443099:fesselin