title: Multi-ingredients determination and fingerprint analysis of leaves from Ilex latifolia using ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry.
abstract: An ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-QTOF-MS) method integrating multi-ingredients determination and fingerprint analysis has been established for quality assessment and control of leaves from Ilex latifolia. The method possesses the advantages of speediness, efficiency, accuracy, and allows the multi-ingredients determination and fingerprint analysis in one chromatographic run within 13min. Multi-ingredients determination was performed based on the extracted ion chromatograms of the exact pseudo-molecular ions (with a 0.01Da window), and fingerprint analysis was performed based on the base peak chromatograms, obtained by negative-ion electrospray ionization QTOF-MS. The method validation results demonstrated our developed method possessing desirable specificity, linearity, precision and accuracy. The method was utilized to analyze 22 I. latifolia samples from different origins. The quality assessment was achieved by using both similarity analysis (SA) and principal component analysis (PCA), and the results from SA were consistent with those from PCA. Our experimental results demonstrate that the strategy integrated multi-ingredients determination and fingerprint analysis using UPLC-QTOF-MS technique is a useful approach for rapid pharmaceutical analysis, with promising prospects for the differentiation of origin, the determination of authenticity, and the overall quality assessment of herbal medicines.
authors.affiliation: Institute of Traditional Chinese Medicine and Natural Products, College of Pharmacy, Jinan University, Guangzhou 510632, PR China.
authors.forename: Chunlin
authors.initials: C
authors.lastname: Fan
authors.forename: Jiewei
authors.initials: J
authors.lastname: Deng
authors.forename: Yunyun
authors.initials: Y
authors.lastname: Yang
authors.forename: Junshan
authors.initials: J
authors.lastname: Liu
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.forename: Xiaoqi
authors.initials: X
authors.lastname: Zhang
authors.forename: Kuokchiu
authors.initials: K
authors.lastname: Fai
authors.forename: Qingwen
authors.initials: Q
authors.lastname: Zhang
authors.forename: Wencai
authors.initials: W
authors.lastname: Ye
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D030017:Ilex / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry; D025341:Principal Component Analysis / Q000379:methods; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
keywords: Fingerprint analysis; Ilex latifolia; Multi-ingredients determination; Quadrupole time-of-flight mass spectrometry; Ultra-performance liquid chromatography