title: Chronic hyperglycemia inhibits vasoregression in a transgenic model of retinal degeneration.
abstract: Vasoregression characterizes diabetic retinopathy in animal models and in humans. We have recently demonstrated that vasoregression is earlier initiated in a rat model of ciliopathy-induced retinal neurodegeneration (TGR rat). The aim was to assess the balance between vasoregressive effects of chronic hyperglycemia and photoreceptor degeneration on adult vascular remodelling. The retinas were analyzed at 4 and 9 months after streptozotocin-induced diabetes. Neurodegeneration was determined by quantitation of cell numbers and retinal layer thickness. Vasoregression was assessed by quantitative retinal morphometry in retinal digest preparations. Retinal VEGF levels were measured by ELISA. Glial activation, expression and location of HSP27 and phosphorylated HSP27 were evaluated by immunofluorescence staining. Unexpectedly, the numbers of acellular capillaries were reduced at both time points and led to fewer intraretinal microvascular abnormalities in late stage diabetic TGR. Concomitantly, inner nuclear layers (INLs) in diabetic TGR rats were protected from cell loss at both time points. Consequently, glial activation was reduced, but VEGF level was increased in diabetic TGR retinas. Expressions of HSP27 were upregulated in glia cells in the preserved INL of diabetic TGR. Chronic hyperglycemia preserves the microvasculature in the retinal model of neurodegeneration. Cell preservation in the retinal INL was associated with protective gene regulation. Together, these data indicate that diabetes can induce vasoprotection, in which retinal glia can play a particular role.
authors.affiliation: 5th Medical Clinic, Faculty of Clinical Medicine, University of Heidelberg, Mannheim, Germany, yuxi.feng@medma.uni-heidelberg.de.
authors.forename: Y
authors.initials: Y
authors.lastname: Feng
authors.forename: Y
authors.initials: Y
authors.lastname: Wang
authors.forename: Z
authors.initials: Z
authors.lastname: Yang
authors.forename: L
authors.initials: L
authors.lastname: Wu
authors.forename: S
authors.initials: S
authors.lastname: Hoffmann
authors.forename: T
authors.initials: T
authors.lastname: Wieland
authors.forename: N
authors.initials: N
authors.lastname: Gretz
authors.forename: H-P
authors.initials: HP
authors.lastname: Hammes
mesh_terms: D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000473:pathology*; D003930:Diabetic Retinopathy / Q000097:blood*; D005260:Female; D055551:HSP27 Heat-Shock Proteins / Q000097:blood; D006651:Histocytochemistry; D006801:Humans; D006943:Hyperglycemia / Q000378:metabolism*; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D009457:Neuroglia / Q000473:pathology; D011897:Random Allocation; D051381:Rats; D055647:Rats, Transgenic; D012162:Retinal Degeneration / Q000097:blood*; D014652:Vascular Diseases / Q000097:blood* / Q000473:pathology; D042461:Vascular Endothelial Growth Factor A / Q000097:blood
chemical_list: D055551:HSP27 Heat-Shock Proteins; C486114:Hspb1 protein, rat; D042461:Vascular Endothelial Growth Factor A; C467498:vascular endothelial growth factor A, rat