title: Analysis of Y chromosome microdeletion in 1738 infertile men from northeastern China.
abstract: OBJECTIVE
  To determine the frequencies and the characteristics of Y chromosome microdeletion in infertile men from northeastern China to perform appropriate therapeutic choices.
  
  
  METHODS
  The study included 1738 infertile men. Sperm concentration was measured according to standard methods and karyotype analysis was performed on peripheral blood lymphocytes with standard G-banding. Multiplex polymerase chain reaction amplification using 9 specific sequence-tagged sites were selected to detect Y chromosome microdeletions.
  
  
  RESULTS
  The data showed that the frequency of Y chromosome microdeletion was 8.57%. The most common microdeletion, among the azoospermia factor (AZF) regions, was detected in the AZFc region, followed by AZFb+c, AZFb, AZFa+b+c, AZFa, and AZFa+c. One-hundred seven patients with Y chromosome microdeletion developed azoospermia, 39 developed severe oligozoospermia (sperm concentration ≤5 × 10(6)/mL), and 3 developed moderate oligozoospermia (sperm concentration >5 × 10(6)/mL and ≤10 × 10(6)/mL). Karyotype analysis was available for 130 patients with Y chromosome microdeletion and abnormal karyotypes were found in 19 patients (14.6%). The most frequent abnormal karyotype was 46,X,Yqh-(n = 7).
  
  
  CONCLUSIONS
  In northeastern China, Y chromosome microdeletion diagnosis should be performed before the use of intracytoplasmic sperm injection in infertile men with sperm count ≤10 × 10(6)/mL, especially in men with azoospermia.
authors.affiliation: Centre for Reproductive Medicine, First Hospital of Jilin University, Changchun, China.
authors.forename: Yong-Sheng
authors.initials: YS
authors.lastname: Zhang
authors.forename: Ru-Lin
authors.initials: RL
authors.lastname: Dai
authors.forename: Rui-Xue
authors.initials: RX
authors.lastname: Wang
authors.forename: Hong-Guo
authors.initials: HG
authors.lastname: Zhang
authors.forename: Shuang
authors.initials: S
authors.lastname: Chen
authors.forename: Rui-Zhi
authors.initials: RZ
authors.lastname: Liu
mesh_terms: D000328:Adult; D044466:Asian People / Q000235:genetics*; D053713:Azoospermia / Q000235:genetics*; D002681:China; D002872:Chromosome Deletion; D041322:Chromosomes, Human, Y / Q000235:genetics*; D006801:Humans; D059785:Karyotype; D008297:Male; D008875:Middle Aged; D009845:Oligospermia / Q000235:genetics*; D012729:Sex Chromosome Aberrations; D013076:Sperm Count; D055815:Young Adult