title: Upgraded ESRF BM29 beamline for SAXS on macromolecules in solution.
abstract: Small-angle X-ray scattering (SAXS) measurements of proteins in solution are becoming increasingly popular with biochemists and structural biologists owing to the presence of dedicated high-throughput beamlines at synchrotron sources. As part of the ESRF Upgrade program a dedicated instrument for performing SAXS from biological macromolecules in solution (BioSAXS) has been installed at the renovated BM29 location. The optics hutch has been equipped with new optical components of which the two principal elements are a fixed-exit double multilayer monochromator and a 1.1 m-long toroidal mirror. These new dedicated optics give improved beam characteristics (compared with the previous set-up on ID14-3) regarding the energy tunability, flux and focusing at the detector plane leading to reduced parasitic scattering and an extended s-range. User experiments on the beamline have been successfully carried out since June 2012. A description of the new BioSAXS beamline and the set-up characteristics are presented together with examples of obtained data.
authors.affiliation: ESRF, 6 Jules Horowitz, F-38043 Grenoble, France. rejma@esrf.fr
authors.forename: Petra
authors.initials: P
authors.lastname: Pernot
authors.forename: Adam
authors.initials: A
authors.lastname: Round
authors.forename: Ray
authors.initials: R
authors.lastname: Barrett
authors.forename: Alejandro
authors.initials: A
authors.lastname: De Maria Antolinos
authors.forename: Alexandre
authors.initials: A
authors.lastname: Gobbo
authors.forename: Elspeth
authors.initials: E
authors.lastname: Gordon
authors.forename: Julien
authors.initials: J
authors.lastname: Huet
authors.forename: Jerôme
authors.initials: J
authors.lastname: Kieffer
authors.forename: Mario
authors.initials: M
authors.lastname: Lentini
authors.forename: Muriel
authors.initials: M
authors.lastname: Mattenet
authors.forename: Christian
authors.initials: C
authors.lastname: Morawe
authors.forename: Christoph
authors.initials: C
authors.lastname: Mueller-Dieckmann
authors.forename: Staffan
authors.initials: S
authors.lastname: Ohlsson
authors.forename: Werner
authors.initials: W
authors.lastname: Schmid
authors.forename: John
authors.initials: J
authors.lastname: Surr
authors.forename: Pascal
authors.initials: P
authors.lastname: Theveneau
authors.forename: Louiza
authors.initials: L
authors.lastname: Zerrad
authors.forename: Sean
authors.initials: S
authors.lastname: McSweeney
mesh_terms: D011506:Proteins / Q000737:chemistry*; D053838:Scattering, Small Angle; D012996:Solutions
chemical_list: D011506:Proteins; D012996:Solutions
keywords: automation and high throughput; online HPLC; proteins in solution; small-angle X-ray scattering; structural biology