title: Pierisformotoxins A-D, polyesterified grayanane diterpenoids from Pieris formosa and their cAMP-decreasing activities.
abstract: Four highly acylated diterpenoids, designated as pierisformotoxins A-D (1-4, resp.), along with 26 known compounds, were isolated from the flowers of Pieris formosa. Among them, pierisformotoxins A and B (1 and 2, resp.) were new highly acylated grayanane diterpenoids, of which the five-membered ring A has undergone an oxidative cleavage between C(3) and C(4), followed by lactonization, to give rise to a five-membered lactone ring between C(3) and C(5), differing from the previously reported grayanane diterpenoids with a 5/7/6/5 ring system. Results of the cAMP-regulation-activity assay showed that pierisformotoxin C (3) at 10 μM (inhibitory ratio (IR): 10.1%) or 2 μM (9.8%), and pierisformotoxin B (2) at 50 μM (13.9%) significantly decreased the cAMP level in N1E-115 neuroblastoma cells (p<0.05).
authors.affiliation: The Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650050, PR China.
authors.forename: Wei-Guang
authors.initials: WG
authors.lastname: Wang
authors.forename: Zhao-Yuan
authors.initials: ZY
authors.lastname: Wu
authors.forename: Rui
authors.initials: R
authors.lastname: Chen
authors.forename: Hai-Zhou
authors.initials: HZ
authors.lastname: Li
authors.forename: Hong-Mei
authors.initials: HM
authors.lastname: Li
authors.forename: Yuan-Dan
authors.initials: YD
authors.lastname: Li
authors.forename: Rong-Tao
authors.initials: RT
authors.lastname: Li
authors.forename: Huai-Rong
authors.initials: HR
authors.lastname: Luo
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D000242:Cyclic AMP / Q000378:metabolism; D004224:Diterpenes / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D029791:Ericaceae / Q000737:chemistry*; D035264:Flowers / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D008968:Molecular Conformation; D009474:Neurons / Q000187:drug effects
chemical_list: D004224:Diterpenes; D000242:Cyclic AMP