title: Analysis of gene expression on anodic porous alumina microarrays.
abstract: This paper investigates the application of anodic porous alumina as an advancement on chip laboratory for gene expressions. The surface was prepared by a suitable electrolytic process to obtain a regular distribution of deep micrometric holes and printed bypen robot tips under standard conditions. The gene expression within the Nucleic Acid Programmable Protein Array (NAPPA) is realized in a confined environment of 16 spots, containing circular DNA plasmids expressed using rabbit reticulocyte lysate. Authors demonstrated the usefulness of APA in withholding the protein expression by detecting with a CCD microscope the photoluminescence signal emitted from the complex secondary antibody anchored to Cy3 and confined in the pores. Friction experiments proved the mechanical resistance under external stresses by the robot tip pens printing. So far, no attempts have been made to directly compare APA with any other surface/substrate; the rationale for pursuing APA as a potential surface coating is that it provides advantages over the simple functionalization of a glass slide, overcoming concerns about printing and its ability to generate viable arrays.
authors.affiliation: Nanoworld Institute; Fondazione EL.B.A. Nicolini; Pradalunga, Italy; Laboratories of Biophysics and Nanobiotechnology; Department of Experimental Medicine; University of Genova; Genova, Italy; Department of Surgical Sciences and Integrated Diagnostic; University of Genova; Genova, Italy.
authors.forename: Claudio
authors.initials: C
authors.lastname: Nicolini
authors.forename: Manjul
authors.initials: M
authors.lastname: Singh
authors.forename: Rosanna
authors.initials: R
authors.lastname: Spera
authors.forename: Lamberto
authors.initials: L
authors.lastname: Felli
mesh_terms: D000537:Aluminum Oxide / Q000737:chemistry*; D000818:Animals; D000906:Antibodies / Q000737:chemistry; D002232:Carbocyanines; D004566:Electrodes; D005456:Fluorescent Dyes; D015870:Gene Expression; D008163:Luminescent Measurements / Q000295:instrumentation* / Q000379:methods; D010957:Plasmids; D016062:Porosity; D040081:Protein Array Analysis / Q000295:instrumentation* / Q000379:methods; D011817:Rabbits; D012156:Reticulocytes / Q000737:chemistry / Q000378:metabolism
chemical_list: D000906:Antibodies; D002232:Carbocyanines; D005456:Fluorescent Dyes; C089187:cyanine dye 3; D000537:Aluminum Oxide
keywords: NAPPA; anodic porous alumina array; genes expression