title: In vitro antioxidant activities of methanol and aqueous extract of Annona squamosa (L.) fruit pulp.
abstract: The present study evaluated the antioxidant activity of the fruit of Annona squamosa by means of in vitro studies involving two different solvent extracts: methanol and aqueous. The antioxidant properties of the extract were determined by scavenging 1, 1-diphenyl-2-picrylhydrazyl radical (DPPH), lipid peroxidation (LPO), nitric oxide (NO), superoxide anion (O2(-)), hydroxyl radical (OH(•)), reducing power and total antioxidant. The results showed that, compared to aqueous extract, a methanolic fruit extract of A squamosa has a higher percentage of inhibition of DPPH radical scavenging activity (97.99%), LPO (94.15%), NO scavenging activity (70.96%), O2(-) scavenging activity and OH(•) scavenging activity (78.68% and 85.25%, respectively), total antioxidant activity (206 μg α-tocopherol/g) and reducing power (56.0 μg of ascorbic acid/g). The results obtained in the in vitro models clearly suggest that methanol extract has higher antioxidant activity than the aqueous extract due to a higher presence of phenolic and flavonodal constituents in the methanol extract.
authors.affiliation: Department of Biochemistry, New Prince Shri Bhavani College of Arts and Science, University of Madras, Tamilnadu, India.
authors.forename: Elumalai
authors.initials: E
authors.lastname: Nandhakumar
authors.forename: Parameswaran
authors.initials: P
authors.lastname: Indumathi
mesh_terms: D031041:Annona / Q000737:chemistry*; D016166:Free Radical Scavengers / Q000737:chemistry* / Q000302:isolation & purification; D005638:Fruit / Q000737:chemistry*; D017665:Hydroxyl Radical / Q000737:chemistry; D015227:Lipid Peroxidation; D010084:Oxidation-Reduction; D010936:Plant Extracts / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: D016166:Free Radical Scavengers; D010936:Plant Extracts; D017665:Hydroxyl Radical