title: Biosynthesis of gold and silver nanoparticles using a novel marine strain of Stenotrophomonas.
abstract: The present study aims at exploiting marine microbial diversity for biosynthesis of metal nanoparticles and also investigates role of microbial proteins in the process of bio-mineralization of gold and silver. This is the first report for concurrent production of gold and silver nanoparticles (AuNPs and AgNPs) by extracellular secretion of a novel strain of Stenotrophomonas, isolated from Indian marine origin. This novel strain has faster rate kinetics for AgNPs synthesis than any other organism reported earlier. The nanoparticles were further characterized using UV-vis spectrophotometer, TEM, DLS and EDAX confirming their size ranging from 10-50 nm and 40-60 nm in dimensions for AuNPs and AgNPs, respectively. TEM analysis indicated formation of multi-shaped nanoparticles with heterogeneous size distribution in both the cases. Finally, the SDS-PAGE analysis of extracellular media supernatant suggested a potential involvement of certain low molecular weight secretory proteins in AuNPs and AgNPs biosynthesis.
authors.affiliation: CSIR-Institute of Microbial Technology (IMTECH), Council of Scientific and Industrial Research (CSIR), Sector 39A, Chandigarh 160 036, India.
authors.forename: Ankit
authors.initials: A
authors.lastname: Malhotra
authors.forename: Kunzes
authors.initials: K
authors.lastname: Dolma
authors.forename: Navjot
authors.initials: N
authors.lastname: Kaur
authors.forename: Y S
authors.initials: YS
authors.lastname: Rathore
authors.lastname: Ashish
authors.forename: S
authors.initials: S
authors.lastname: Mayilraj
authors.forename: Anirban Roy
authors.initials: AR
authors.lastname: Choudhury
mesh_terms: D004591:Electrophoresis, Polyacrylamide Gel; D006046:Gold / Q000737:chemistry*; D008386:Marine Biology; D053758:Nanoparticles / Q000737:chemistry*; D010802:Phylogeny; D012834:Silver / Q000737:chemistry*; D020588:Stenotrophomonas / Q000145:classification / Q000378:metabolism*
chemical_list: D012834:Silver; D006046:Gold
keywords: Fermentation; Gold nanoparticle; Marine microbe; Silver nanoparticle; Stenotrophomonas