title: Segregation and efficacy of the cry1Ia1 gene for control of potato tuberworm in four populations of cultivated potato.
abstract: The potato (Solanum tuberosum L.) cultivar 'SpuntaG2' contains a single copy of the Bacillus thuringiensis (Bt) cry1Ia1 gene and controls potato tuberworm (Phthorimaea operculella Zeller, Lepidoptera: Gelechiidae). Two potato cultivars and two breeding lines were crossed with SpuntaG2 creating four populations used to study cry1Ia1 segregation and efficacy. The cry1Ia1 gene segregated in each of the four populations with a 1:1 ratio. All progeny that were polymerase chain reaction positive for the cry1Ia1 gene had no surviving larvae and no leaf mining in detached leaf assays after 72 h. These results support previous evidence that SpuntaG2 carries a single copy of the cry1Ia1 gene and that transmission of the transgene from parent to progeny is not restricted and follows expected Mendelian segregation ratios. Based on detached leaf assays, the efficacy of the cry1Ia1 gene is retained through sexual transmission. If the SpuntaG2 cry1Ia1 insertion event is deregulated for commercial use, SpuntaG2 could be used for conventional breeding and the progeny carrying the SpuntaG2 event would also be available for commercial use.
authors.affiliation: Department of Plant, Soil and Microbial Sciences, Michigan State University, Plant and Soil Science Bldg., 1066 Bogue Street, Rm 2100, East Lansing, MI 48824, USA. douchesd@msu.edu
authors.forename: David S
authors.initials: DS
authors.lastname: Douches
authors.forename: Joseph J
authors.initials: JJ
authors.lastname: Coombs
authors.forename: Kimberly J
authors.initials: KJ
authors.lastname: Felcher
authors.forename: Matthew
authors.initials: M
authors.lastname: Zuehlke
authors.forename: Walter
authors.initials: W
authors.lastname: Pett
authors.forename: Zsofia
authors.initials: Z
authors.lastname: Szendrei
authors.forename: Johan
authors.initials: J
authors.lastname: Brink
mesh_terms: D000818:Animals; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D020090:Chromosome Segregation; D004731:Endotoxins / Q000235:genetics* / Q000378:metabolism; D006460:Hemolysin Proteins / Q000235:genetics* / Q000378:metabolism; D007814:Larva / Q000254:growth & development / Q000502:physiology; D009036:Moths / Q000254:growth & development / Q000502:physiology*; D010572:Pest Control, Biological; D030821:Plants, Genetically Modified / Q000235:genetics* / Q000502:physiology; D016133:Polymerase Chain Reaction; D011198:Solanum tuberosum / Q000235:genetics* / Q000502:physiology; D019076:Transgenes
chemical_list: D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D004731:Endotoxins; D006460:Hemolysin Proteins; C030956:insecticidal crystal protein, Bacillus Thuringiensis