title: The skin secretion of the amphibian Phyllomedusa nordestina: a source of antimicrobial and antiprotozoal peptides.
abstract: Antimicrobial peptides (AMPs) from the dermaseptin and phylloseptin families were isolated from the skin secretion of Phyllomedusa nordestina, a recently described amphibian species from Northeastern Brazil. One dermaseptin and three phylloseptins were chosen for solid phase peptide synthesis. The antiprotozoal and antimicrobial activities of the synthetic peptides were determined, as well as their cytotoxicity in mouse peritoneal cells. AMPs are being considered as frameworks for the development of novel drugs inspired by their mechanism of action.
authors.affiliation: Laboratório de Espectrometria de Massa, EMBRAPA, Recursos Genéticos e Biotecnologia, Brasília, DF 70770-900, Brazil.
authors.forename: Guilherme D
authors.initials: GD
authors.lastname: Brand
authors.forename: Raimunda C
authors.initials: RC
authors.lastname: Santos
authors.forename: Luisa Mayumi
authors.initials: LM
authors.lastname: Arake
authors.forename: Valdelânia G
authors.initials: VG
authors.lastname: Silva
authors.forename: Leiz M C
authors.initials: LM
authors.lastname: Veras
authors.forename: Vladimir
authors.initials: V
authors.lastname: Costa
authors.forename: Carlos Henrique N
authors.initials: CH
authors.lastname: Costa
authors.forename: Selma S
authors.initials: SS
authors.lastname: Kuckelhaus
authors.forename: José Guilherme
authors.initials: JG
authors.lastname: Alexandre
authors.forename: Maria J
authors.initials: MJ
authors.lastname: Feio
authors.forename: José Roberto S A
authors.initials: JR
authors.lastname: Leite
mesh_terms: D029845:Amphibian Proteins / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D000818:Animals; D000890:Anti-Infective Agents / Q000737:chemistry / Q000494:pharmacology; D023181:Antimicrobial Cationic Peptides / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology*; D000981:Antiprotozoal Agents / Q000737:chemistry / Q000494:pharmacology; D001001:Anura; D008264:Macrophages / Q000187:drug effects; D051379:Mice; D008826:Microbial Sensitivity Tests; D012867:Skin / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D029845:Amphibian Proteins; D000890:Anti-Infective Agents; D023181:Antimicrobial Cationic Peptides; D000981:Antiprotozoal Agents; C070905:dermaseptin