title: Fluorescence detection of glutathione reductase activity based on deoxyribonucleic acid-templated silver nanoclusters.
abstract: Fluorescent silver nanoclusters stabilized by DNA (DNA-AgNCs) exhibit distinct response rates to thiol and disulfide. Glutathione reductase can catalyze the reduction of the oxidized glutathione (GSSG) quickly to reduced glutathione (GSH) in the presence of β-nicotinamide adenine dinucleotide 2'-phosphate reduced tetrasodium salt hydrate (NADPH). Consequently, DNA-AgNCs can serve as a new fluorescent platform for assaying the glutathione reductase (GR) activity. This newly proposed assay has a high sensitivity and a good selectivity toward GR. The GR activity can be detected in the range of 0.2-2.0 mU mL(-1) with a minimum detectable concentration of 0.2 mU mL(-1). Pepsin, lysozyme, trypsin, avidin, thrombin, myoglobin, and BSA have little effect on the fluorescence intensity of DNA-AgNCs. The GR activity assay is successfully used to monitor the inhibition of GR activity by a typical inhibitor 1,3-bis(2-chloroethyl)-1-nitrosourea.
authors.affiliation: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, Jilin, China.
authors.forename: Shuyun
authors.initials: S
authors.lastname: Zhu
authors.forename: Xian-en
authors.initials: XE
authors.lastname: Zhao
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: Zhongyuan
authors.initials: Z
authors.lastname: Liu
authors.forename: Wenjing
authors.initials: W
authors.lastname: Qi
authors.forename: Saima
authors.initials: S
authors.lastname: Anjum
authors.forename: Guobao
authors.initials: G
authors.lastname: Xu
mesh_terms: D004247:DNA / Q000737:chemistry*; D004789:Enzyme Activation / Q000502:physiology; D005980:Glutathione Reductase / Q000032:analysis* / Q000378:metabolism; D053758:Nanoparticles / Q000737:chemistry*; D012834:Silver / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000379:methods / Q000592:standards
chemical_list: D012834:Silver; D004247:DNA; D005980:Glutathione Reductase