title: Generation of anti-sulfated glycan antibodies using sulfotransferase-deficient mice.
abstract: Anti-carbohydrate monoclonal antibodies (mAbs) are very useful in the functional analysis of complex carbohydrates in vivo. However, such mAbs are difficult to generate, largely because a wide variety of complex carbohydrates is intrinsically expressed in mice and rats and because the antigenicities of glycans are generally poor. In this chapter, I describe an efficient method for generating anti-carbohydrate mAbs using glycan--synthesizing enzyme-knockout mice in which the glycan structures formed by the missing enzymes should be highly antigenic. As an application of this method, I describe the generation of anti-sulfated glycan mAbs using sulfotransferase-deficient mice and the immunohistochemical detection of sulfated glycans involved in lymphocyte homing in both humans and mice.
authors.affiliation: Laboratory of Microbiology and Immunology, School of Pharmaceutical Sciences, University of Shizuoka, Shizuoka, Japan.
authors.forename: Hiroto
authors.initials: H
authors.lastname: Kawashima
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis / Q000276:immunology*; D000917:Antibody Formation; D016466:CHO Cells; D006224:Cricetinae; D003412:Cricetulus; D005455:Fluorescent Antibody Technique / Q000379:methods; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D011134:Polysaccharides / Q000032:analysis / Q000276:immunology*; D013431:Sulfates / Q000032:analysis / Q000276:immunology*; D015238:Sulfotransferases / Q000235:genetics*
chemical_list: D000911:Antibodies, Monoclonal; D011134:Polysaccharides; D013431:Sulfates; D015238:Sulfotransferases