title: Simple and reliable preparation of immunodiagnostic antigens for Taenia solium cysticercosis.
abstract: SUMMARY Cysticercosis caused by infection with the larval stage of Taenia solium is an important cause of neurological disease worldwide and immunodiagnosis is important for the control and elimination of cysticercosis. In the present study, we established a simple and reliable preparation of immunodiagnostic low-molecular-weight antigens (LMWAgs) from T. solium cyst fluids by a cation-exchange chromatography (CEC). Banding patterns of LMWAgs on SDS-PAGE were different between isolates from Ecuador and China. All cysticercosis patient sera and some echinococcosis patient sera recognized both LMWAgs by enzyme-linked immunosorbent assay (ELISA), but sera from healthy persons were not positive. There was no statistical difference in immunodiagnostic performance of LMWAgs prepared from different geographical isolates. These results indicated that these novel immunodiagnostic antigen preparations could contribute the control and prevention of cysticercosis in endemic areas, especially developing countries.
authors.affiliation: Department of Parasitology, Asahikawa Medical University, Asahikawa, Hokkaido, Japan.
authors.forename: Yasuhito
authors.initials: Y
authors.lastname: Sako
authors.forename: Sonoyo
authors.initials: S
authors.lastname: Itoh
authors.forename: Munehiro
authors.initials: M
authors.lastname: Okamoto
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Nakaya
authors.forename: Akira
authors.initials: A
authors.lastname: Ito
mesh_terms: D000818:Animals; D000947:Antigens, Helminth / Q000032:analysis*; D002681:China; D002852:Chromatography, Ion Exchange; D003551:Cysticercosis / Q000175:diagnosis* / Q000276:immunology / Q000469:parasitology / Q000662:veterinary*; D003906:Developing Countries; D004484:Ecuador; D006801:Humans; D007118:Immunoassay; D007814:Larva / Q000737:chemistry / Q000276:immunology; D008970:Molecular Weight; D011933:Reagent Kits, Diagnostic; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis* / Q000276:immunology / Q000469:parasitology; D041201:Taenia solium / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification*
chemical_list: D000947:Antigens, Helminth; D011933:Reagent Kits, Diagnostic