title: Genome-wide profiling of human cap-independent translation-enhancing elements.
abstract: We report an in vitro selection strategy to identify RNA sequences that mediate cap-independent initiation of translation. This method entails mRNA display of trillions of genomic fragments, selection for initiation of translation and high-throughput deep sequencing. We identified >12,000 translation-enhancing elements (TEEs) in the human genome, generated a high-resolution map of human TEE-bearing regions (TBRs), and validated the function of a subset of sequences in vitro and in cultured cells.
authors.affiliation: Center for Evolutionary Medicine and Informatics in the Biodesign Institute, Arizona State University, Tempe, Arizona, USA.
authors.forename: Brian P
authors.initials: BP
authors.lastname: Wellensiek
authors.forename: Andrew C
authors.initials: AC
authors.lastname: Larsen
authors.forename: Bret
authors.initials: B
authors.lastname: Stephens
authors.forename: Kim
authors.initials: K
authors.lastname: Kukurba
authors.forename: Karl
authors.initials: K
authors.lastname: Waern
authors.forename: Natalia
authors.initials: N
authors.lastname: Briones
authors.forename: Li
authors.initials: L
authors.lastname: Liu
authors.forename: Michael
authors.initials: M
authors.lastname: Snyder
authors.forename: Bertram L
authors.initials: BL
authors.lastname: Jacobs
authors.forename: Sudhir
authors.initials: S
authors.lastname: Kumar
authors.forename: John C
authors.initials: JC
authors.lastname: Chaput
mesh_terms: D020121:5' Untranslated Regions; D015723:Gene Library; D015894:Genome, Human; D006367:HeLa Cells; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D010442:Peptide Chain Initiation, Translational; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism
chemical_list: D020121:5' Untranslated Regions; D012333:RNA, Messenger