title: Raman endoscopy for the in situ investigation of advancing colorectal tumors in live model mice.
abstract: The aim of the present study is to evaluate the capability of a miniaturized Raman endoscope (mRE) system to monitor the advancement of colorectal tumors in model mice as a method that is noninvasive to the tumor itself. Nevertheless, the endoscope is narrow enough to observe the inside of the mouse colon in such a way that is semi-noninvasive to the animal. However, the mRE system allowed the visualization and Raman spectral measurement of any targeted point within the colorectal tumor in model mice under anesthesia, without damaging the tissue (i.e., noninvasively). Continuous monitoring of the same tumor allowed the observation of alterations in its molecular composition and size, along with its advancement. The tumor lesion was discriminated from normal tissues of the control mouse with an accuracy of 86.8%. We succeeded in visualizing and performing Raman spectral observations on a shrinking polyp type tumor. The Raman analysis suggested that it was not cured but supposedly transformed to another tumor type.
authors.affiliation: Department of Bioscience, School of Science and Technology, Kwansei Gakuin University, 2-1, Gakuen, Sanda, Hyogo 669-1337, Japan.
authors.forename: Akinori
authors.initials: A
authors.lastname: Taketani
authors.forename: Retno
authors.initials: R
authors.lastname: Hariyani
authors.forename: Mika
authors.initials: M
authors.lastname: Ishigaki
authors.forename: Bibin B
authors.initials: BB
authors.lastname: Andriana
authors.forename: Hidetoshi
authors.initials: H
authors.lastname: Sato
mesh_terms: D000367:Age Factors; D000818:Animals; D001397:Azoxymethane / Q000633:toxicity; D002273:Carcinogens / Q000633:toxicity; D003110:Colonic Neoplasms / Q000139:chemically induced / Q000473:pathology*; D016264:Dextran Sulfate / Q000633:toxicity; D004195:Disease Models, Animal; D018450:Disease Progression; D051379:Mice; D008807:Mice, Inbred BALB C; D013059:Spectrum Analysis, Raman / Q000379:methods*
chemical_list: D002273:Carcinogens; D016264:Dextran Sulfate; D001397:Azoxymethane