title: Development of a solid phase microextraction protocol for the GC-MS determination of volatile off-flavour compounds from citral degradation in oil-in-water emulsions.
abstract: The conditions for headspace solid phase microextraction (HS-SPME) analysis of volatile off-flavour compounds in citral emulsion were determined. Type of SPME phase (65 μm PDMS/DVB, 100 μm PDMS and 75μm CAR/PDMS), adsorption temperature and salt concentration were significant factors affecting total peak area in the gas chromatogram and optimised in one factor experiments. Then, adsorption temperature (30-50°C), adsorption time (20-40 min), and salt concentration (0-6M) were studied to develop HS-SPME condition for obtaining the highest extraction efficiency. PDMS/DVB in 65 μm was the optimum fiber because of high adsorption efficiency and good reproducibility. The optimal condition was adsorption at 50°C for 40 min and 6M salt added to sample. Good Linearity, high recovery, good reproducibility and low limit of detection (LOD) for all off-odour compounds according to the optimised SPME conditions indicated that the SPME procedure was applicable for the analysis of the degraded citral products in headspace volatile of emulsion.
authors.affiliation: Department of Food Science and Technology, Shanghai Institute of Technology, 120 Caobao Road, 200235 Shanghai, China.
authors.forename: Huaixiang
authors.initials: H
authors.lastname: Tian
authors.forename: Xiaoqing
authors.initials: X
authors.lastname: Yang
authors.forename: Chi-Tang
authors.initials: CT
authors.lastname: Ho
authors.forename: Qingrong
authors.initials: Q
authors.lastname: Huang
authors.forename: Shiqing
authors.initials: S
authors.lastname: Song
mesh_terms: D000080462:Acyclic Monoterpenes; D004655:Emulsions / Q000737:chemistry; D005421:Flavoring Agents / Q000032:analysis / Q000302:isolation & purification*; D008401:Gas Chromatography-Mass Spectrometry; D039821:Monoterpenes / Q000032:analysis / Q000302:isolation & purification*; D052617:Solid Phase Microextraction / Q000379:methods*; D055549:Volatile Organic Compounds / Q000032:analysis / Q000302:isolation & purification*
chemical_list: D000080462:Acyclic Monoterpenes; D004655:Emulsions; D005421:Flavoring Agents; D039821:Monoterpenes; D055549:Volatile Organic Compounds; C007076:citral