title: Preparation and characterization of a biologic scaffold from esophageal mucosa.
abstract: Biologic scaffolds composed of extracellular matrix (ECM) are commonly used to facilitate a constructive remodeling response in several types of tissue, including the esophagus. Surgical manipulation of the esophagus is often complicated by stricture, but preclinical and clinical studies have shown that the use of an ECM scaffold can mitigate stricture and promote a constructive outcome after resection of full circumference esophageal mucosa. Recognizing the potential benefits of ECM derived from homologous tissue (i.e., site-specific ECM), the objective of the present study was to prepare, characterize, and assess the in-vivo remodeling properties of ECM from porcine esophageal mucosa. The developed protocol for esophageal ECM preparation is compliant with previously established criteria of decellularization and results in a scaffold that maintains important biologic components and an ultrastructure consistent with a basement membrane complex. Perivascular stem cells remained viable when seeded upon the esophageal ECM scaffold in-vitro, and the in-vivo host response showed a pattern of constructive remodeling when implanted in soft tissue.
authors.affiliation: McGowan Institute for Regenerative Medicine, University of Pittsburgh, Pittsburgh, PA 15219, USA; Department of Bioengineering, University of Pittsburgh, Pittsburgh, PA 15219, USA.
authors.forename: Timothy J
authors.initials: TJ
authors.lastname: Keane
authors.forename: Ricardo
authors.initials: R
authors.lastname: Londono
authors.forename: Ryan M
authors.initials: RM
authors.lastname: Carey
authors.forename: Christopher A
authors.initials: CA
authors.lastname: Carruthers
authors.forename: Janet E
authors.initials: JE
authors.lastname: Reing
authors.forename: Christopher L
authors.initials: CL
authors.lastname: Dearth
authors.forename: Antonio
authors.initials: A
authors.lastname: D'Amore
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Medberry
authors.forename: Stephen F
authors.initials: SF
authors.lastname: Badylak
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D004947:Esophagus / Q000737:chemistry*; D005109:Extracellular Matrix / Q000737:chemistry* / Q000648:ultrastructure; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D009092:Mucous Membrane / Q000737:chemistry*; D051381:Rats; D013552:Swine; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry*