title: Tinnitus, unipolar brush cells, and cerebellar glutamatergic function in an animal model.
abstract: Unipolar brush cells (UBCs) are excitatory interneurons found in the dorsal cochlear nucleus (DCN) and the granule cell layer of cerebellar cortex, being particularly evident in the paraflocculus (PFL) and flocculus (FL). UBCs receive glutamatergic inputs and make glutamatergic synapses with granule cells and other UBCs. It has been hypothesized that UBCs comprise local networks of tunable feed-forward amplifiers. In the DCN they might also participate in feed-back amplification of signals from higher auditory centers. Recently it has been shown that UBCs, in the vestibulocerebellum and DCN of adult rats, express doublecortin (DCX), previously considered a marker of newborn and migrating neurons. In an animal model, both the DCN, and more recently the PFL, have been implicated in contributing to the sensation of acoustic-exposure-induced tinnitus. These studies support the working hypothesis that tinnitus emerges after loss of peripheral sensitivity because inhibitory processes homeostatically down regulate, and excitatory processes up regulate. Here we report the results of two sequential experiments that examine the potential role of DCN and cerebellar UBCs in tinnitus, and the contribution of glutamatergic transmission in the PFL. In Experiment 1 it was shown that adult rats with psychophysical evidence of tinnitus induced by a single unilateral high-level noise exposure, had elevated DCX in the DCN and ventral PFL. In Experiment 2 it was shown that micro-quantities of glutamatergic antagonists, delivered directly to the PFL, reversibly reduced chronically established tinnitus, while similarly applied glutamatergic agonists induced tinnitus-like behavior in non-tinnitus controls. These results are consistent with the hypothesis that UBC up regulation and enhanced glutamatergic transmission in the cerebellum contribute to the pathophysiology of tinnitus.
authors.affiliation: Department of Otolaryngology, Southern Illinois University School of Medicine, Springfield, Illinois, United States of America.
authors.forename: Carol A
authors.initials: CA
authors.lastname: Bauer
authors.forename: Kurt W
authors.initials: KW
authors.lastname: Wisner
authors.forename: Joan S
authors.initials: JS
authors.lastname: Baizer
authors.forename: Thomas J
authors.initials: TJ
authors.lastname: Brozoski
mesh_terms: D000818:Animals; D002525:Cerebellar Cortex / Q000166:cytology / Q000187:drug effects / Q000503:physiopathology*; D017626:Cochlear Nucleus / Q000166:cytology / Q000187:drug effects / Q000503:physiopathology*; D004195:Disease Models, Animal; D000089662:Doublecortin Protein; D018690:Excitatory Amino Acid Agonists / Q000008:administration & dosage / Q000494:pharmacology; D018691:Excitatory Amino Acid Antagonists / Q000008:administration & dosage / Q000494:pharmacology; D005971:Glutamates / Q000378:metabolism*; D007395:Interneurons / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D051381:Rats; D013569:Synapses / Q000378:metabolism*; D014012:Tinnitus / Q000188:drug therapy / Q000378:metabolism* / Q000503:physiopathology*
chemical_list: C000714848:Dcx protein, rat; D000089662:Doublecortin Protein; D018690:Excitatory Amino Acid Agonists; D018691:Excitatory Amino Acid Antagonists; D005971:Glutamates