title: [Effect of acetic acid, furfural and 5-hydroxymethylfurfural on production of 2,3-butanediol by Klebsiella oxytoca].
abstract: To get the tolerability and consumption of Klebsiella oxytoca on major inhibitors in lignocelluloses hydrolysate, we studied the effect of acetic acid, furfural and 5-hydroxymethylfurfural on production of 2,3-butanediol by Klebsiella oxytoca. The metabolites of furfural and 5-hydroxymethylfurfural were measured. The results show that when acetic acid, furfural and 5-hydroxymethylfurfural was individually added, tolerance threshold for Klebsiella oxytoca was 30 g/L, 4 g/L and 5 g/L, respectively. Acetic acid was likely used as substrate to produce 2,3-butanediol. The yield of 2,3-butanediol increased when acetic acid concentration was lower than 30 g/L. In the fermentation, more than 70% 5-hydroxymethylfurfural was converted to 2,5-furandimethanol. All furfural and the rest of 5-hydroxymethylfurfural were metabolized by Klebsiella oxytoca. It showed that in the detoxification process of 2,3-butanediol production using lignocelluloses hydrolysate, furfural should be given priority to remove and a certain concentration of acetic acid is not need to removal.
authors.affiliation: National and Provincial Joint Key Laborary of Coal Science and Technology of Nurturing Base, Taiyuan University of Technology, Taiyuan 030024, Shanxi, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Wu
authors.forename: Keke
authors.initials: K
authors.lastname: Cheng
authors.forename: Wenying
authors.initials: W
authors.lastname: Li
authors.forename: Jie
authors.initials: J
authors.lastname: Feng
authors.forename: Jian'an
authors.initials: J
authors.lastname: Zhang
mesh_terms: D019342:Acetic Acid / Q000737:chemistry*; D002072:Butylene Glycols / Q000378:metabolism*; D005285:Fermentation; D005662:Furaldehyde / Q000031:analogs & derivatives* / Q000737:chemistry*; D041121:Klebsiella oxytoca / Q000378:metabolism*; D008031:Lignin / Q000737:chemistry / Q000378:metabolism
chemical_list: D002072:Butylene Glycols; C036909:lignocellulose; C026978:2,3-butylene glycol; C008046:5-hydroxymethylfurfural; D008031:Lignin; D005662:Furaldehyde; D019342:Acetic Acid