title: An artificial restriction DNA cutter for site-selective gene insertion in human cells.
abstract: With the use of a chemistry-based artificial restriction DNA cutter (combination of Ce(IV)-EDTA and a pair of pcPNA), both an antibiotic-resistance gene and a fluorescent reporter protein gene were incorporated into the targeted site through homologous recombination in human cells.
authors.affiliation: Research Center for Advanced Science and Technology, The University of Tokyo, 4-6-1 Komaba, Tokyo 153-8904, Japan.
authors.forename: Kenichiro
authors.initials: K
authors.lastname: Ito
authors.forename: Narumi
authors.initials: N
authors.lastname: Shigi
authors.forename: Makoto
authors.initials: M
authors.lastname: Komiyama
mesh_terms: D002460:Cell Line; D002563:Cerium / Q000737:chemistry / Q000378:metabolism; D004247:DNA / Q000008:administration & dosage* / Q000235:genetics / Q000378:metabolism; D004352:Drug Resistance, Microbial; D004492:Edetic Acid / Q000737:chemistry / Q000378:metabolism; D018274:Electroporation; D059765:Homologous Recombination; D006801:Humans; D016254:Mutagenesis, Insertional; D020135:Peptide Nucleic Acids / Q000737:chemistry / Q000378:metabolism; D011506:Proteins / Q000235:genetics; D014162:Transfection / Q000379:methods*
chemical_list: D020135:Peptide Nucleic Acids; D011506:Proteins; D002563:Cerium; D004247:DNA; D004492:Edetic Acid