title: Determination of biotin in Antarctic krill (Euphausia superba) by high-performance TLC with different post-chromatographic derivatizations.
abstract: A new efficient method was developed to detect biotin in Antarctic krill by Vis-absorbance detection. DMF was used after chloroform pretreatment to extract biotin and two chromogenic methods were developed. The development system consisted of dichloromethane/dimethylcarbinol/methanol/glacial acetic acid (3:3:2:0.015, v/v/v/v). Samples were separated on precoated silica gel GF254 high-performance TLC plates. Densitometric analysis of biotin was carried out in the absorbance mode at 400 and 530 nm. The biotin content was determined to be 1.0948 ± 0.0097 and 1.1212 ± 0.0155 mg/g in Antarctic krill with the two chromogenic methods, which had no significant difference.
authors.affiliation: Key Laboratory of Animal Resistance, College of Life Science, Shandong Normal University, Jinan, PR China.
authors.forename: Peishan
authors.initials: P
authors.lastname: Teo
authors.forename: Daicheng
authors.initials: D
authors.lastname: Liu
mesh_terms: D000818:Animals; D001710:Biotin / Q000032:analysis* / Q000302:isolation & purification; D005591:Chemical Fractionation; D002855:Chromatography, Thin Layer / Q000379:methods*; D033201:Euphausiacea / Q000737:chemistry*
chemical_list: D001710:Biotin
keywords: 4-(Dimethylamino)cinnamaldehyde; Antarctic krill; Biotin; High performance; Potassium permanganate; TLC