title: Structure of a galactoarabinoglucuronoxylan from tamarillo (Solanum betaceum), a tropical exotic fruit, and its biological activity.
abstract: Tamarillo (Solanum betaceum) is a tropical exotic fruit whose polysaccharides were extracted from the ripe pulp. After various purification steps, homogeneous fractions (designated PTW, STK-1000R and PF) were analyzed by sugar composition, HPSEC, methylation and NMR spectroscopy analysis. The results showed that the fraction PTW consisted of a linear arabinan with (1→5)-linked α-l-arabinofuranosyl units. Fractions designated as STK-1000R and PF contained galactoarabinoglucuronoxylans, with (1→4)-linked β-d-Xylp residues in the backbone, carrying branches exclusively at O-2. The polysaccharide in STK-1000R is less branched than that in the PF fraction (∼20.0% and 36.5%, respectively), with side-chains formed by (1→5)-linked α-l-Araf residues and (1→4)-linked α-d-GlcpA residues and with non-reducing end units formed by α-l-Araf, β-Arap, β-d-Galp, α-d-GlcpA and 4-O-Me-α-d-GlcpA. Intraperitoneal administration of the STK-1000R fraction in mice significantly reduced the number of abdominal constrictions induced by 0.6% acetic acid and the inflammatory phase of nociception induced by 2.5% formalin, indicating that that fraction has an antinociceptive effect on inflammatory pain models.
authors.affiliation: Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, CP 19.046, CEP 81.531-980 Curitiba, PR, Brazil.
authors.forename: Georgia Erdmann
authors.initials: GE
authors.lastname: do Nascimento
authors.forename: Leticia Alencar
authors.initials: LA
authors.lastname: Hamm
authors.forename: Cristiane H
authors.initials: CH
authors.lastname: Baggio
authors.forename: Maria Fernanda de Paula
authors.initials: MF
authors.lastname: Werner
authors.forename: Marcello
authors.initials: M
authors.lastname: Iacomini
authors.forename: Lucimara M C
authors.initials: LM
authors.lastname: Cordeiro
mesh_terms: D000818:Animals; D002240:Carbohydrate Sequence; D003085:Colic / Q000188:drug therapy; D005260:Female; D005638:Fruit / Q000737:chemistry*; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D015394:Molecular Structure; D059225:Nociception / Q000187:drug effects; D010936:Plant Extracts / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D032322:Solanum / Q000737:chemistry*
chemical_list: D010936:Plant Extracts; D011134:Polysaccharides