title: Reconstruction of radial bone defect using gelatin sponge and a BMP-2 combination graft.
abstract: Many bioactive molecules like recombinant human bone morphogenetic protein 2 (rhBMP-2) have been developed for mineralized bone grafts, for which proper scaffolds are necessary to successfully apply the bioactive molecules. In this study, we tested the osteogenic efficacy of rhBMP-2 produced in-house in combination with gelatin sponge as the scaffold carrier in a rabbit radial defect model. The efficacy of the rhBMP-2 was determined by alkaline phosphatase activity assay of C2C12 cells. Two groups of ten rabbits each were treated with rhBMP-2/gelatin sponge, or gelatin sponge only. At 4 weeks, rhBMP-2/gelatin sponge grafts showed more bone regeneration than gelatin sponge grafts, as determined by X-ray radiography, micro-computed tomography, and histological analyses. At 8 weeks, rhBMP-2/gelatin sponge grafts exerted much stronger osteogenic effects. The study demonstrates the improved osteogenic efficacy of the rhBMP-2/gelatin sponge grafts in a rabbit radial bone defect model acting as a bone-inductive material.
authors.affiliation: Department of Oral and Maxillofacial Surgery, College of Dentistry, Gangneung-Wonju National University, Gangneung 210-702, Korea.
authors.forename: Seong-Gon
authors.initials: SG
authors.lastname: Kim
authors.forename: Jae-Hwan
authors.initials: JH
authors.lastname: Jeong
authors.forename: Xiangguo
authors.initials: X
authors.lastname: Che
authors.forename: Yong-Tae
authors.initials: YT
authors.lastname: Park
authors.forename: Sang-Woon
authors.initials: SW
authors.lastname: Lee
authors.forename: Eun-Sun
authors.initials: ES
authors.lastname: Jung
authors.forename: Senyon
authors.initials: S
authors.lastname: Choe
authors.forename: Je-Yong
authors.initials: JY
authors.lastname: Choi
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D055396:Bone Morphogenetic Protein 2 / Q000494:pharmacology*; D001861:Bone Regeneration / Q000187:drug effects*; D002460:Cell Line; D050659:Core Binding Factor Alpha 1 Subunit / Q000378:metabolism; D005780:Gelatin / Q000737:chemistry*; D006801:Humans; D051379:Mice; D023421:Models, Animal; D011817:Rabbits; D011884:Radius / Q000000981:diagnostic imaging / Q000473:pathology*; D011994:Recombinant Proteins / Q000494:pharmacology; D051785:Smad Proteins / Q000378:metabolism; D014057:Tomography, X-Ray Computed; D016212:Transforming Growth Factor beta / Q000494:pharmacology*
chemical_list: D055396:Bone Morphogenetic Protein 2; D050659:Core Binding Factor Alpha 1 Subunit; D011994:Recombinant Proteins; D051785:Smad Proteins; D016212:Transforming Growth Factor beta; C499469:recombinant human bone morphogenetic protein-2; D005780:Gelatin; D000469:Alkaline Phosphatase