title: An experimental assessment of toxic potential of nanoparticle preparation of heavy metals in streptozotocin induced diabetes.
abstract: Nanoparticle preparations of heavy metals have attracted enormous scientific and technological interest. Biologically produced nanoparticle preparations of heavy metals are elaborately described in traditional texts and being widely prescribed. The underlying interactions of nano preparations within the physiological fluids are key feature to understand their biological impact. In this perspective, we performed an experimental assessment of the toxicity potential of a marketed metallic preparation named Vasant Kusumakar Ras (VKR), wherein different heavy metals in composite form are reduced to nanoparticle size to produce the desired effect in diabetes and its complications. VKR (50mg/kg) was administered to Albino Wistar rats rendered diabetic using streptozotocin (90mg/kg) in 2 days old neonates. Anti-hyperglycemic effect was observed with VKR along with increased levels of plasma insulin. Renal variables including total proteins and albumin along with glomerular filtration rate were found to improve biochemically. The results were supplemented by effects on different inflammatory and growth factors like TNF-α, nitric oxide, TGF-β and VEGF. However, the results observed in kidney histopathology were not in accordance with the biochemical parameters. Inflammation observed in kidney was confirmed by immunostaining metallothionein, which was due to the accumulation of heavy metals. Furthermore, mercury accumulation in kidney further confirmed by autometallography, which activated mononuclear phagocyte system, which generated an immune response. This was further supported by increase in the extent of apoptosis in kidney tissues. In conclusion, nanoparticle preparations of heavy metals can be toxic to kidney if it is not regulated with respect to its surface chemistry and dosage.
authors.affiliation: Delhi Institute of Pharmaceutical Sciences and Research, Pushp Vihar, Sector-3, MB Road, New Delhi 110017, India. Electronic address: sonia.gandhi@rediffmail.com.
authors.forename: Sonia
authors.initials: S
authors.lastname: Gandhi
authors.forename: B P
authors.initials: BP
authors.lastname: Srinivasan
authors.forename: Atul Sureshrao
authors.initials: AS
authors.lastname: Akarte
mesh_terms: D000818:Animals; D053938:DNA Fragmentation / Q000187:drug effects; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy*; D005919:Glomerular Filtration Rate / Q000187:drug effects; D007150:Immunohistochemistry; D007328:Insulin / Q000097:blood; D007668:Kidney / Q000187:drug effects*; D008515:Medicine, Ayurvedic; D053768:Metal Nanoparticles / Q000633:toxicity*; D019216:Metals, Heavy / Q000633:toxicity*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D007328:Insulin; D019216:Metals, Heavy
keywords: Apoptosis; Autometallography; Diabetic nephropathy; Mercury; Metallothionein; Nephrin