title: Isolation, Identification, and Characterization of a Cellulolytic Bacillus amyloliquefaciens Strain SS35 from Rhinoceros Dung.
abstract: Cellulose hydrolyzing bacteria were isolated from rhinoceros dung and tested for clear zone formation around the colonies on the agar plates containing the medium amended with carboxymethylcellulose as a sole carbon source. Isolates were further screened on the basis of carboxymethylcellulase production in liquid medium. Out of 36 isolates, isolate no. 35 exhibited maximum enzyme activity of 0.079 U/mL and was selected for further identification by using conventional biochemical tests and phylogenetic analyses. This was a Gram-positive, spore forming bacterium with rod-shaped cells. The isolate was identified as Bacillus amyloliquefaciens SS35 based on nucleotide homology and phylogenetic analysis using 16S rDNA and gyrase A gene sequences.
authors.affiliation: Center for Energy, Indian Institute of Technology Guwahati, Guwahati, Assam 781 039, India.
authors.forename: Shuchi
authors.initials: S
authors.lastname: Singh
authors.forename: Vijayanand S
authors.initials: VS
authors.lastname: Moholkar
authors.forename: Arun
authors.initials: A
authors.lastname: Goyal