title: Development and validation of a stability indicating RP-HPLC method for simultaneous estimation of Olmesartan Medoxomil and Metoprolol Succinate in pharmaceutical dosage form.
abstract: OBJECTIVE
  A simple, rapid, precise and isocratic RP-HPLC (Reverse Phase High Performance Liquid Chromatography) method is aimed to develop for the simultaneous estimation of Olmesartan Medoxomil and Metoprolol Succinate in bulk drug and pharmaceutical dosage form.
  
  
  METHODS
  The quantification is carried out using YMC-Pack CN (250 × 4.6 mm, 5.0 μm) column and the mobile phase comprises of 0.05% Trifluoro acetic acid (TFA) and Acetonitrile (ACN) (70:30 v/v). The flow rate is 1.0 ml/min. The eluent is monitored at 220 nm. The retention times of Olmesartan Medoxomil and Metoprolol Succinate are 7.9 min and 4.1 min respectively. The method is validated in terms of linearity, precision, accuracy, specificity, limit of detection and limit of quantitation.
  
  
  RESULTS
  Linearity and percentage recoveries of both Olmesartan Medoxomil and Metoprolol Succinate are in the range of 5-35 μg/ml and 100 ± 2%, respectively. The stress testing of both the drugs individually and their mixture is carried out under acidic, alkaline, oxidation, photo-stability and thermal degradation (dry heat and wet heat) conditions and its degradation products are well resolved from the analyte peaks.
  
  
  CONCLUSIONS
  This method was successfully validated for accuracy, precision, and linearity.
authors.affiliation: Department of Pharmaceutical Analysis and Quality Assurance, MAEER's Maharashtra Institute of Pharmacy, S. No. 124, MIT Campus, Ex-serviceman Colony, Paud Road, Kothrud, Pune, Maharashtra, India.
authors.forename: Nirmal M
authors.initials: NM
authors.lastname: Thakker
authors.forename: Haresh B
authors.initials: HB
authors.lastname: Panchal
authors.forename: Dinesh R
authors.initials: DR
authors.lastname: Rakholiya
authors.forename: R
authors.initials: R
authors.lastname: Murugan
authors.forename: Vishnu P
authors.initials: VP
authors.lastname: Choudhari
authors.forename: Bhanudas S
authors.initials: BS
authors.lastname: Kuchekar
keywords: HPLC; Metoprolol Succinate; Olmesartan Medoxomil; stability indicating method