title: In vivo inhibition of trans-plasma membrane electron transport by antiviral drugs in grapevine.
abstract: Electrophysiological techniques were applied to investigate the action of antiviral drugs during trans-plasma events in in vivo grapevine cells infected by GLRaV-1 and GLRaV-3. Carbon fiber microelectrodes and redox-sensitive dyes were used to measure trans-plasma membrane electron transport (t-PMET) activity in healthy and infected samples treated with ribavirin, tiazofurin and oseltamivir. Each drug caused a reduction in oxidation current (expressed as Δ[Fe(2+)]) in healthy samples, indicating t-PMET inhibition. In almost all infected samples, the effect of drugs on t-PMET activity was significantly lower, suggesting that higher content of NADH in infected plants can interfere with t-PMET inhibition caused by drugs. Moreover, virus-infected samples exhibited elevated t-PMET activity compared to healthy samples. Analogous effects were observed by dye tests. Considering the effects of drugs on trans-plasma membrane potential, tests showed the activity of a proton pump during drug treatments with no significant difference with regard to health status.
authors.affiliation: Department of Agriculture, Food and Environment, University of Pisa, Via del Borghetto, 80, 56124 Pisa, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Panattoni
authors.forename: E
authors.initials: E
authors.lastname: Rinaldelli
authors.forename: E
authors.initials: E
authors.lastname: Triolo
authors.forename: A
authors.initials: A
authors.lastname: Luvisi
mesh_terms: D000998:Antiviral Agents / Q000494:pharmacology*; D004579:Electron Transport / Q000187:drug effects*; D004789:Enzyme Activation / Q000187:drug effects; D008564:Membrane Potentials / Q000187:drug effects; D026901:Membrane Transport Proteins / Q000378:metabolism*; D053139:Oseltamivir / Q000494:pharmacology; D010084:Oxidation-Reduction / Q000187:drug effects; D010088:Oxidoreductases / Q000378:metabolism; D010940:Plant Proteins / Q000037:antagonists & inhibitors* / Q000378:metabolism; D012254:Ribavirin / Q000031:analogs & derivatives / Q000494:pharmacology; D027843:Vitis
chemical_list: D000998:Antiviral Agents; D026901:Membrane Transport Proteins; D010940:Plant Proteins; D053139:Oseltamivir; D012254:Ribavirin; D010088:Oxidoreductases; C033706:tiazofurin