title: Matrix metalloproteinase, hyaluronidase and elastase inhibitory potential of standardized extract of Centella asiatica.
abstract: BACKGROUND
  Centella asiatica (L.) Urban (Apiaceae), a valuable herb described in Ayurveda, is used in the indigenous system of medicine as a tonic to treat skin diseases.
  
  
  OBJECTIVE
  Centella asiatica methanol extract and its ethyl acetate, n-butanol and aqueous fraction, were subjected for the evaluation of skin care potential through the in vitro hyaluronidase, elastase and matrix metalloproteinase-1 (MMP-1) inhibitory assay.
  
  
  METHODS
  The C. asiatica plant was extracted with methanol and fractionated with ethyl acetate, n-butanol and water. The enzymatic activities were evaluated using ursolic acid and oleanolic acid as standards. Isolate molecule asiaticoside was quantified in the crude extract and fractions through high-performance liquid chromatography (HPLC) and structural was characterized by liquid chromatography-mass spectroscopy (LC-MS) and ¹H nuclear magnetic resonance (NMR). Isolated compound was also evaluated for in vitro enzyme assays.
  
  
  RESULTS
  Extract exhibited anti-hyaluronidase and anti-elastase activity with IC₅₀ of 19.27 ± 0.37 and 14.54 ± 0.39 µg/mL, respectively, as compared to ursolic acid. Centella asiatica n-butanol fraction (CAnB) and isolated compound showed significant hyaluronidase (IC₅₀ = 27.00 ± 0.43 and 18.63 ± 0.33 µg/mL) and elastase (IC₅₀ = 29.15 ± 0.31 and 19.45 ± 0.25 µg/mL) inhibitory activities, respectively, and also showed significant MMP-1 inhibition (p < 0.05 and p < 0.01).
  
  
  CONCLUSIONS
  n-Butanol fraction was found to be most effective among the all fractions from which asiaticoside was isolated and further quantified by HPLC. This work concludes that the asiaticoside from C. asiatica may be a prospective agent for skin care.
authors.affiliation: School of Natural Product Studies, Department of Pharmaceutical Technology, Jadavpur University, Kolkata, West Bengal, India.
authors.forename: Neelesh Kumar
authors.initials: NK
authors.lastname: Nema
authors.forename: Niladri
authors.initials: N
authors.lastname: Maity
authors.forename: Birendra Kumar
authors.initials: BK
authors.lastname: Sarkar
authors.forename: Pulok Kumar
authors.initials: PK
authors.lastname: Mukherjee
mesh_terms: D020001:1-Butanol / Q000737:chemistry; D000818:Animals; D002417:Cattle; D028041:Centella / Q000737:chemistry*; D003879:Dermatologic Agents / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D004791:Enzyme Inhibitors / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D020834:Ethnopharmacology; D006801:Humans; D006821:Hyaluronoglucosaminidase / Q000037:antagonists & inhibitors* / Q000378:metabolism; D007194:India; D007700:Kinetics; D019272:Leukocyte Elastase / Q000037:antagonists & inhibitors* / Q000378:metabolism; D020781:Matrix Metalloproteinase 1 / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D061965:Matrix Metalloproteinase Inhibitors / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D008515:Medicine, Ayurvedic; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D012997:Solvents / Q000737:chemistry; D014315:Triterpenes / Q000032:analysis / Q000302:isolation & purification / Q000494:pharmacology
chemical_list: C526146:Centella asiatica extract; D003879:Dermatologic Agents; D004791:Enzyme Inhibitors; D061965:Matrix Metalloproteinase Inhibitors; D010936:Plant Extracts; D011994:Recombinant Proteins; D012997:Solvents; D014315:Triterpenes; D020001:1-Butanol; D006821:Hyaluronoglucosaminidase; D019272:Leukocyte Elastase; C522360:MMP1 protein, human; D020781:Matrix Metalloproteinase 1; C004446:asiaticoside