title: Antiviral activity of Jinchai capsule against influenza virus.
abstract: OBJECTIVE
  To evaluate the effect on influenza virus of Jinchai, a capsule made of Traditional Chinese Medicine.
  
  
  METHODS
  Madin-darby canine kidney (MDCK) cells were infected with the FM1 strain of influenza virus A (subtype H1N1) in vitro. They were used to explore how Jinchai affected cell adsorption, cell membrane fusion, transcription and replication of the influenza virus. Hemagglutinin (HA) protein, intracellular pH, and influenza virus protein acid (PA) polymerase subunit were detected with confocal microscopy and real-time fluorescent quantitative polymerase chain reaction.
  
  
  RESULTS
  Jinchai significantly reduced the expression of HA and PA polymerase subunit mRNA in infected MDCK cells. Jinchai also significantly decreased intracellular pH in infected cells.
  
  
  CONCLUSIONS
  Jinchai had strong anti-influenza activity against the influenza virus. It weakened the ability of the influenza virus to adsorb to cell wall and fuse with cell membranes in the early infection stage, and inhibited the transcription and replication of the virus.
authors.affiliation: Experimental Research Center, China Academy of Chinese Medical Sciences, Beijing 100700, China.
authors.forename: Juying
authors.initials: J
authors.lastname: Zhong
authors.forename: Xiaolan
authors.initials: X
authors.lastname: Cui
authors.forename: Yujing
authors.initials: Y
authors.lastname: Shi
authors.forename: Yingjie
authors.initials: Y
authors.lastname: Gao
authors.forename: Hongxin
authors.initials: H
authors.lastname: Cao
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology*; D002460:Cell Line; D004285:Dogs; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D015967:Gene Expression Regulation, Viral / Q000187:drug effects; D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype / Q000187:drug effects* / Q000502:physiology; D007251:Influenza, Human / Q000821:virology*; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism; D014779:Virus Replication / Q000187:drug effects
chemical_list: D000998:Antiviral Agents; D004365:Drugs, Chinese Herbal; D014764:Viral Proteins