title: Concentration of biologically active compounds extracted from Ilex paraguariensis St. Hil. by nanofiltration.
abstract: The aim of this study was to characterise the bioactive compounds in mate (Ilex paraguariensis St. Hil) extract and in concentrated mate extract obtained by nanofiltration (NF). Also, the impact of NF on the antioxidant activity of both mate extracts was evaluated in vitro and using eukaryotic cells of Saccharomyces cerevisiae (yeast assay). The results showed a significant increase in the contents of total phenolics (338%), chlorogenic acid (483%), theobromine (323%), caffeine (251%), chlorophyll (321%), condensed tannins (278%) and saponins (211%) in the concentrated mate extract. The concentrated mate extract showed higher in vitro antioxidant activity than the mate extract. According to the results obtained, it can be stated that the use of nanofiltration membrane is a valid approach for the concentration of biologically active compounds in aqueous extract of mate.
authors.affiliation: Department of Food Science and Technology, Federal University of Santa Catarina, Rodovia Admar Gonzaga, 1346, 88034-001 Florianópolis, SC, Brazil.
authors.forename: Aureanna Nairne Negrão
authors.initials: AN
authors.lastname: Murakami
authors.forename: Renata Dias de Mello Castanho
authors.initials: RD
authors.lastname: Amboni
authors.forename: Elane Schwinden
authors.initials: ES
authors.lastname: Prudêncio
authors.forename: Edna Regina
authors.initials: ER
authors.lastname: Amante
authors.forename: Carlise Beddin
authors.initials: CB
authors.lastname: Fritzen-Freire
authors.forename: Brunna Cristina Bremer
authors.initials: BC
authors.lastname: Boaventura
authors.forename: Isabella de Bona
authors.initials: Ide B
authors.lastname: Muñoz
authors.forename: Catia Dos Santos
authors.initials: Cdos S
authors.lastname: Branco
authors.forename: Miriam
authors.initials: M
authors.lastname: Salvador
authors.forename: Marcelo
authors.initials: M
authors.lastname: Maraschin
mesh_terms: D002110:Caffeine / Q000032:analysis / Q000302:isolation & purification; D002726:Chlorogenic Acid / Q000032:analysis / Q000302:isolation & purification; D005374:Filtration / Q000295:instrumentation / Q000379:methods*; D030019:Ilex paraguariensis / Q000737:chemistry*; D010936:Plant Extracts / Q000032:analysis / Q000302:isolation & purification*; D012503:Saponins / Q000032:analysis / Q000302:isolation & purification
chemical_list: D010936:Plant Extracts; D012503:Saponins; D002726:Chlorogenic Acid; D002110:Caffeine