title: [Which molecular biology techniques must conform to the armamentarium for basic research in uro-oncology?].
abstract: Molecular biology has been one of the scientific disciplines in which there has been more advances in the last years. The first impulse in the study of genetic alterations came from the discovery of DNA structure, followed by elucidation of the genetic code, the discovery of restriction enzymes and subsequently the invention of PCR, not forgetting the exponential development of computer science. All of them have allowed us to know much more about our genome and its regulation than we could imagine. The impulse in proteomics has been especially in tune up of soft methods of ionization coupled with mass spectrometry. Nevertheless, this seems to be only the beginning since today there are continuous methodological advances that will increase more, without doubt, the knowledge and applications in this discipline.
authors.affiliation: Servicio de Bioquímica y Genética Molecular, CDB, Hospital Clínico, Departamento de Ciencias Fisiológicas I, Facultad de Medicina, UB, Barcelona, España. joriola@clinic.ub.es
authors.forename: Josep
authors.initials: J
authors.lastname: Oriola
mesh_terms: D000818:Animals; D035843:Biomedical Research; D004247:DNA / Q000235:genetics; D004273:DNA, Neoplasm / Q000235:genetics; D006801:Humans; D008495:Medical Oncology / Q000379:methods*; D008967:Molecular Biology / Q000379:methods*; D009363:Neoplasm Proteins / Q000235:genetics; D012313:RNA / Q000235:genetics; D012334:RNA, Neoplasm / Q000235:genetics; D014572:Urology / Q000379:methods*
chemical_list: D004273:DNA, Neoplasm; D009363:Neoplasm Proteins; D012334:RNA, Neoplasm; D012313:RNA; D004247:DNA