title: Paraoxonase-1 inhibits oxidized low-density lipoprotein-induced metabolic alterations and apoptosis in endothelial cells: a nondirected metabolomic study.
abstract: We studied the influence of PON1 on metabolic alterations induced by oxidized LDL when incubated with endothelial cells. HUVEC cells were incubated with native LDL, oxidized LDL, oxidized LDL plus HDL from wild type mice, and oxidized LDL plus HDL from PON1-deficient mice. Results showed alterations in carbohydrate and phospholipid metabolism and increased apoptosis in cells incubated with oxidized LDL. These changes were partially prevented by wild type mouse HDL, but the effects were less effective with HDL from PON1-deficient mice. Our results suggest that PON1 may play a significant role in endothelial cell survival by protecting cells from alterations in the respiratory chain induced by oxidized LDL. These results extend current knowledge on the protective role of HDL and PON1 against oxidation and apoptosis in endothelial cells.
authors.affiliation: Unitat de Recerca Biomèdica, Hospital Universitari de Sant Joan, Institut d'Investigació Sanitària Pere Virgili, Universitat Rovira i Virgili, C Sant Joan, 43201 Reus, Catalonia, Spain.
authors.forename: Anabel
authors.initials: A
authors.lastname: García-Heredia
authors.forename: Judit
authors.initials: J
authors.lastname: Marsillach
authors.forename: Anna
authors.initials: A
authors.lastname: Rull
authors.forename: Iris
authors.initials: I
authors.lastname: Triguero
authors.forename: Isabel
authors.initials: I
authors.lastname: Fort
authors.forename: Bharti
authors.initials: B
authors.lastname: Mackness
authors.forename: Michael
authors.initials: M
authors.lastname: Mackness
authors.forename: Diana M
authors.initials: DM
authors.lastname: Shih
authors.forename: Jorge
authors.initials: J
authors.lastname: Joven
authors.forename: Jordi
authors.initials: J
authors.lastname: Camps
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000235:genetics / Q000502:physiology; D043303:Aryldialkylphosphatase / Q000235:genetics / Q000378:metabolism*; D053453:Caspase 9 / Q000378:metabolism; D002952:Citric Acid Cycle / Q000502:physiology; D042783:Endothelial Cells / Q000378:metabolism*; D005434:Flow Cytometry; D006019:Glycolysis / Q000502:physiology; D006801:Humans; D008075:Lipoproteins, HDL / Q000378:metabolism; D008077:Lipoproteins, LDL / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D008817:Mice, Mutant Strains; D010743:Phospholipids / Q000378:metabolism
chemical_list: D008075:Lipoproteins, HDL; D008077:Lipoproteins, LDL; D010743:Phospholipids; C099252:oxidized low density lipoprotein; D043303:Aryldialkylphosphatase; D053453:Caspase 9