title: Arabidopsis BPM proteins function as substrate adaptors to a cullin3-based E3 ligase to affect fatty acid metabolism in plants.
abstract: Regulation of transcriptional processes is a critical mechanism that enables efficient coordination of the synthesis of required proteins in response to environmental and cellular changes. Transcription factors require accurate activity regulation because they play a critical role as key mediators assuring specific expression of target genes. In this work, we show that cullin3-based E3 ligases have the potential to interact with a broad range of ethylene response factor (ERF)/APETALA2 (AP2) transcription factors, mediated by Math-BTB/POZ (for Meprin and TRAF [tumor necrosis factor receptor associated factor] homolog)-Broad complex, Tramtrack, Bric-a-brac/Pox virus and Zinc finger) proteins. The assembly with an E3 ligase causes degradation of their substrates via the 26S proteasome, as demonstrated for the wrinkled1 ERF/AP2 protein. Furthermore, loss of Math-BTB/POZ proteins widely affects plant development and causes altered fatty acid contents in mutant seeds. Overall, this work demonstrates a link between fatty acid metabolism and E3 ligase activities in plants and establishes CUL3-based E3 ligases as key regulators in transcriptional processes that involve ERF/AP2 family members.
authors.affiliation: School of Biological Sciences, Washington State University, Pullman, Washington 99164, USA.
authors.forename: Liyuan
authors.initials: L
authors.lastname: Chen
authors.forename: Joo Hyun
authors.initials: JH
authors.lastname: Lee
authors.forename: Henriette
authors.initials: H
authors.lastname: Weber
authors.forename: Takayuki
authors.initials: T
authors.lastname: Tohge
authors.forename: Sandra
authors.initials: S
authors.lastname: Witt
authors.forename: Sanja
authors.initials: S
authors.lastname: Roje
authors.forename: Alisdair R
authors.initials: AR
authors.lastname: Fernie
authors.forename: Hanjo
authors.initials: H
authors.lastname: Hellmann
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D047369:Chromatin Immunoprecipitation; D044842:Cullin Proteins; D003513:Cycloheximide / Q000494:pharmacology; D005227:Fatty Acids / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D018613:Microscopy, Confocal; D008969:Molecular Sequence Data; D009713:Nucleotidyltransferases / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified; D046988:Proteasome Endopeptidase Complex / Q000378:metabolism; D011485:Protein Binding; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012639:Seeds / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D020798:Two-Hybrid System Techniques; D044767:Ubiquitin-Protein Ligases / Q000235:genetics / Q000378:metabolism*; D000099270:Y-Family DNA Polymerases
chemical_list: D029681:Arabidopsis Proteins; D002352:Carrier Proteins; D044842:Cullin Proteins; D003513:Cycloheximide; D005227:Fatty Acids; D049452:Green Fluorescent Proteins; D009713:Nucleotidyltransferases; D046988:Proteasome Endopeptidase Complex; D014157:Transcription Factors; D044767:Ubiquitin-Protein Ligases; C500789:AT1G26830 protein, Arabidopsis; C586777:BPM1 protein, Arabidopsis; C495665:WRINKLED1 protein, Arabidopsis; C500790:cullin 3B protein, Arabidopsis; C504535:REV1 protein, Arabidopsis; D000099270:Y-Family DNA Polymerases; C061553:ATP dependent 26S protease