title: Cloning, expression, and purification of the hemolysin/cytolysin (HlyE antigen) from Salmonella enterica serovar Typhi: potential application for immunoassay development.
abstract: The hemolysin (HlyE) protein of Salmonella enterica serovar Typhi was reported to be antigenic. This work describes the cloning, expression, and purification of a hexahistidine-tagged HlyE protein under native conditions. Immunoblot analysis and a competitive enzyme-linked immunosorbent assay using sera from typhoid patients showed the presence of HlyE-specific antibodies in circulation.
authors.affiliation: Institute for Research in Molecular Medicine, Universiti Sains Malaysia, 11800 Penang, Malaysia.
authors.forename: Eugene Boon Beng
authors.initials: EB
authors.lastname: Ong
authors.forename: Amy Amilda
authors.initials: AA
authors.lastname: Anthony
authors.forename: Aziah
authors.initials: A
authors.lastname: Ismail
authors.forename: Asma
authors.initials: A
authors.lastname: Ismail
authors.forename: Theam Soon
authors.initials: TS
authors.lastname: Lim
mesh_terms: D000907:Antibodies, Bacterial / Q000097:blood*; D000942:Antigens, Bacterial / Q000235:genetics / Q000302:isolation & purification; D019411:Clinical Laboratory Techniques / Q000379:methods*; D003001:Cloning, Molecular; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D015870:Gene Expression; D006460:Hemolysin Proteins / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D015151:Immunoblotting / Q000379:methods; D011994:Recombinant Proteins / Q000235:genetics / Q000302:isolation & purification; D012485:Salmonella typhi / Q000235:genetics / Q000276:immunology*; D014435:Typhoid Fever / Q000175:diagnosis*
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D006460:Hemolysin Proteins; C531350:HlyE protein, Salmonella enterica; D011994:Recombinant Proteins
keywords: Antigen; ELISA; HlyE; Immunoblot; Salmonella; Typhi