title: A static magnetic field attenuates lipopolysaccharide-induced neuro-inflammatory response via IL-6-mediated pathway.
abstract: An effective method for controlling brain damage and neurodegeneration caused by inflammation remains elusive. Down-expression of the lipopolysaccharide (LPS)-induced inflammatory cytokines resulting in endotoxin tolerance is reported as an alternative anti-infection treatment. Nonetheless, because the dosage and action site are hard to control, endotoxin tolerance caused by low-dose LPS injection in brain tissue may induce side effects. The aim of this study was to test the hypothesis that static magnetic fields (SMF) stimulate endotoxin tolerance in brain tissue. In this study, survival rate and pathological changes in brain tissues of LPS-challenged mice were examined with and without SMF treatment. In addition, the effects of SMF exposure on growth rate and cytokine expression of LPS-challenged BV-2 microglia cells were monitored. Our results showed that SMF pre-exposure had positive effects on the survival rate and histological outcomes of LPS-treated mice. Furthermore, SMF exposure significantly decreased IL-6 expression in BV-2 cells (p < 0.05) by a phenomenon similar to endotoxin tolerance. We suggest that SMF has potential as an alternative simulation source for controlling LPS-induced excess neuro-inflammatory response.
authors.affiliation: Department of Radiology, Shuang Ho Hospital, Taipei Medical University , New Taipei City , Taiwan .
authors.forename: Li-Kuo
authors.initials: LK
authors.lastname: Shen
authors.forename: Haw-Ming
authors.initials: HM
authors.lastname: Huang
authors.forename: Po-Chieh
authors.initials: PC
authors.lastname: Yang
authors.forename: Yung-Kai
authors.initials: YK
authors.lastname: Huang
authors.forename: Peter Da-Yun
authors.initials: PD
authors.lastname: Wang
authors.forename: Ting-Kai
authors.initials: TK
authors.lastname: Leung
authors.forename: Chi-Jen
authors.initials: CJ
authors.lastname: Chen
authors.forename: Wei-Jen
authors.initials: WJ
authors.lastname: Chang
mesh_terms: D000818:Animals; D001921:Brain / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D049109:Cell Proliferation / Q000187:drug effects; D007249:Inflammation / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology; D015850:Interleukin-6 / Q000096:biosynthesis / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000633:toxicity*; D060526:Magnetic Fields; D008297:Male; D051379:Mice; D017628:Microglia / Q000187:drug effects / Q000473:pathology; D015398:Signal Transduction / Q000187:drug effects*; D015996:Survival Rate
chemical_list: D015850:Interleukin-6; D008070:Lipopolysaccharides