title: [Development of ELISA on the basis of monoclonal antibodies for detecting specific activity of the vaccine against hemorrhagic fever with renal syndrome].
abstract: The monoclonal antibodies to Puumala, Dobrava, Hantaan, and Seoul hantaviruses were obtained using mice. The viruses were known to cause HFRS, and two variants of ELISA were designed. First, Hanta-PUU variant, was constructed using monoclonal antibodies to Puumala virus envelope glycoprotein (G(N):G(C)) for detecting only Puumala virus antigen. The second, Hanta-N variant, was constructed using monoclonal antibodies to Dobrava and Puumala nucleocapsid proteins for detecting four above mentioned hantaviruses. Both Hanta-PUU and Hanta-N assays were reliable in detecting specific hantavirus antigens and the immunogenecity of hantavirus vaccines.
authors.forename: T K
authors.initials: TK
authors.lastname: Dzagurova
authors.forename: O N
authors.initials: ON
authors.lastname: Solopova
authors.forename: P G
authors.initials: PG
authors.lastname: Sveshnikov
authors.forename: N A
authors.initials: NA
authors.lastname: Korotina
authors.forename: M V
authors.initials: MV
authors.lastname: Balovneva
authors.forename: O A
authors.initials: OA
authors.lastname: Leonovich
authors.forename: N E
authors.initials: NE
authors.lastname: Varlamov
authors.forename: G A
authors.initials: GA
authors.lastname: Malkin
authors.forename: S E
authors.initials: SE
authors.lastname: Sotskova
authors.forename: E A
authors.initials: EA
authors.lastname: Tkachenko
mesh_terms: D000818:Animals; D058846:Antibodies, Monoclonal, Murine-Derived / Q000737:chemistry* / Q000276:immunology; D000956:Antigens, Viral / Q000276:immunology*; D002522:Chlorocebus aethiops; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006476:Orthohantavirus / Q000276:immunology*; D006480:Hemorrhagic Fever with Renal Syndrome / Q000276:immunology*; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D014709:Vero Cells; D014759:Viral Envelope Proteins / Q000276:immunology*; D014765:Viral Vaccines / Q000276:immunology*
chemical_list: D058846:Antibodies, Monoclonal, Murine-Derived; D000956:Antigens, Viral; D014759:Viral Envelope Proteins; D014765:Viral Vaccines