title: Thermogenesis is involved in the body-fat lowering effects of resveratrol in rats.
abstract: The effect of resveratrol on thermogenesis in skeletal muscle and interscapular brown adipose tissue (IBAT) was investigated. Rats were fed an obesogenic diet supplemented with resveratrol (30mg/kg/day) or not supplemented for 6weeks. Resveratrol intake led to increased gene expression of mitochondrial-transcription-factor-A (TFAM), mitochondrial-protein-cytochrome-C-oxidase subunit-2 (COX2), sirtuin-1 (SIRT1), peroxisome-proliferator-activated-receptor-β/δ (PPARβ/δ) and proliferator-activated-receptor-gamma-coactivator1-α (PGC-1α) in IBAT and increased UCP1protein expression; however, peroxisome-proliferator-activated-receptor-α (PPARα) expression remained unchanged. In gastrocnemius muscle, resveratrol increased the gene expression of TFAM and COX2; however, no changes were observed in levels of SIRT1, PGC-1α and PPARβ/δ. Acetylated-PGC-1α was decreased in the resveratrol-treated group, indicating a higher level of activation, and a significant increase of UCP3 protein expression was observed in this group. The increases in UCP protein expression in two important thermogenic tissues after resveratrol treatment may contribute to increased whole-body energy dissipation, which may help to better understand the body-fat lowering effect of this polyphenol.
authors.affiliation: Nutrition and Obesity Group, Department of Nutrition and Food Science, University of the Basque Country (UPV/EHU), Paseo de la Universidad, 7, 01006 Vitoria, Spain. ggooiiuurrii@yahoo.es
authors.forename: Goiuri
authors.initials: G
authors.lastname: Alberdi
authors.forename: Víctor M
authors.initials: VM
authors.lastname: Rodríguez
authors.forename: Jonatan
authors.initials: J
authors.lastname: Miranda
authors.forename: M Teresa
authors.initials: MT
authors.lastname: Macarulla
authors.forename: Itziar
authors.initials: I
authors.lastname: Churruca
authors.forename: María P
authors.initials: MP
authors.lastname: Portillo
mesh_terms: D002001:Adipose Tissue, Brown / Q000187:drug effects* / Q000502:physiology*; D000818:Animals; D003576:Electron Transport Complex IV / Q000235:genetics / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D008297:Male; D018482:Muscle, Skeletal / Q000187:drug effects* / Q000378:metabolism; D009765:Obesity / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism / Q000503:physiopathology*; D047493:PPAR alpha / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D000077185:Resveratrol; D056564:Sirtuin 1 / Q000235:genetics / Q000378:metabolism; D013267:Stilbenes / Q000008:administration & dosage*; D022722:Thermogenesis / Q000187:drug effects*
chemical_list: D047493:PPAR alpha; D013267:Stilbenes; D003576:Electron Transport Complex IV; D056564:Sirtuin 1; D000077185:Resveratrol