title: [Purification and identification of microcystin-RR].
abstract: An effective method based on the combination of solid phase extraction and gel chromatography to extract and purify microcystin-RR (MC-RR) from natural cyanobacteria bloom was developed. Thirty five gram natural cyanobacterial bloom scum was extracted with 70% aqueous methanol. After the methanol in the crude extract was removed by a rotary evaporator, the solid phase extraction was used for preliminary purification and concentration. And then 7.5 mL eluent was concentrated to 2 mL and loaded onto a Sephadex LH-20 gel chromatographic column for further purification. The eluate was collected by tubes and detected at 238 nm. The elution curve was plotted by tube numbers as X-axis and the absorbance at 238 nm as Y-axis. The purity and the spectral characteristics of the final extract were identified with high performance liquid chromatograph (HPLC) and spectrophotometer, respectively. A total of 3.65 mg of MC-RR with a final yield of 74. 1% was obtained, and the purity was more than 90% with characteristic UV absorption spectra at 238 nm.
authors.affiliation: Laboratory of Environment and Health of Ministry of Education. Institute of Environmental Medicine, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
authors.forename: Yonglu
authors.initials: Y
authors.lastname: Zhang
authors.forename: Xianing
authors.initials: X
authors.lastname: Huang
authors.forename: Wenqing
authors.initials: W
authors.lastname: Xiao
authors.forename: Qing
authors.initials: Q
authors.lastname: Zhong
authors.forename: Kangding
authors.initials: K
authors.lastname: Gu
mesh_terms: D002850:Chromatography, Gel / Q000379:methods*; D000458:Cyanobacteria / Q000737:chemistry; D008387:Marine Toxins; D052998:Microcystins / Q000302:isolation & purification*; D052616:Solid Phase Extraction / Q000379:methods*
chemical_list: D008387:Marine Toxins; D052998:Microcystins; C063855:microcystin RR