title: Oligomerization of DHHC protein S-acyltransferases.
abstract: The formation of dimers or higher-order oligomers is a property of numerous integral membrane proteins, including ion channels, transporters, and receptors. In this study, we examined whether members of the DHHC-S-acyltransferase family oligomerize in intact cells and in vitro. DHHC-S-acyltransferases are integral membrane proteins that catalyze the addition of palmitate to cysteine residues on proteins at the cytoplasmic face of cell membranes. Bioluminescence resonance energy transfer (BRET) experiments revealed that DHHC2 or DHHC3 (Golgi-specific DHHC zinc finger protein (GODZ)) self-associate when expressed in HEK-293 cells. Homomultimer formation was confirmed by coimmunoprecipitation. Purified DHHC3 resolved predominately as a monomer and dimer on blue native polyacrylamide gels. In intact cells and in vitro, catalytically inactive DHHC proteins displayed a greater propensity to form dimers. BRET signals were higher for the catalytically inactive DHHC2 or DHHC3 than their wild-type counterparts. DHHC3 BRET in cell membranes was decreased by the addition of its lipid substrate palmitoyl-CoA, a treatment that results in autoacylation of the enzyme. Enzyme activity of a covalently linked DHHC3 dimer was less than that of the monomeric form, suggesting that enzyme activity may be modulated by the oligomerization status of the protein.
authors.affiliation: Department of Molecular Medicine, Cornell University College of Veterinary Medicine, Ithaca, New York 14853, USA.
authors.forename: Jianbin
authors.initials: J
authors.lastname: Lai
authors.forename: Maurine E
authors.initials: ME
authors.lastname: Linder
mesh_terms: D000123:Acetyltransferases / Q000737:chemistry* / Q000378:metabolism; D001704:Biopolymers / Q000737:chemistry / Q000378:metabolism*; D057809:HEK293 Cells; D006801:Humans; D063347:Native Polyacrylamide Gel Electrophoresis
chemical_list: D001704:Biopolymers; D000123:Acetyltransferases; C062782:protein acyltransferase
keywords: Bioluminescence Resonance Energy Transfer (BRET); Enzyme Structure; Fatty Acid; Membrane Enzymes; Palmitoylation; Posttranslational Modification