title: Sampling of wheat dust and subsequent analysis of deoxynivalenol by LC-MS/MS.
abstract: An LC-MS/MS method was developed and validated for the determination of deoxynivalenol in wheat dust. Extraction was carried out with acetonitrile/water/acetic acid (79/20/1, v/v/v) followed by a hexane defatting step. Analysis was performed using a Waters Acquity UPLC system coupled to a Quattro Premier XE mass spectrometer. The method was validated according to the criteria mentioned in Commission Decision 2002/657/EC. Due to a high contamination level of wheat dust compared to wheat, limit of detection and limit of quantitation levels of 358 ng/g and 717 ng/g, respectively, were obtained. A small survey was executed on raw wheat materials and their corresponding dust samples (n = 12). The samples were analyzed according to the developed procedure. A linear correlation (R² = 0.941) was found for the deoxynivalenol concentration in dust versus the deoxynivalenol concentration in wheat. Therefore, it would be possible to estimate the cereal contamination through dust contamination.
authors.affiliation: Department of Bioanalysis, Faculty of Pharmaceutical Sciences, Ghent University, Harelbekestraat 72, 9000 Ghent, Belgium. melanie.sanders@ugent.be
authors.forename: Melanie
authors.initials: M
authors.lastname: Sanders
authors.forename: Marthe
authors.initials: M
authors.lastname: De Boevre
authors.forename: Frédéric
authors.initials: F
authors.lastname: Dumoulin
authors.forename: Christ'l
authors.initials: C
authors.lastname: Detavernier
authors.forename: Freya
authors.initials: F
authors.lastname: Martens
authors.forename: Christof
authors.initials: C
authors.lastname: Van Poucke
authors.forename: Mia
authors.initials: M
authors.lastname: Eeckhout
authors.forename: Sarah
authors.initials: S
authors.lastname: De Saeger
mesh_terms: D001530:Belgium; D004391:Dust / Q000032:analysis*; D005433:Flour / Q000032:analysis / Q000191:economics; D005506:Food Contamination; D005513:Food Inspection / Q000379:methods*; D005525:Food-Processing Industry / Q000191:economics; D007220:Industrial Waste / Q000032:analysis / Q000191:economics; D009183:Mycotoxins / Q000032:analysis* / Q000737:chemistry; D012639:Seeds / Q000737:chemistry*; D014255:Trichothecenes / Q000032:analysis* / Q000737:chemistry; D014908:Triticum / Q000737:chemistry*
chemical_list: D004391:Dust; D007220:Industrial Waste; D009183:Mycotoxins; D014255:Trichothecenes; C007262:deoxynivalenol