title: Low density supercritical fluids precipitation of 9-cis and all trans-β-carotenes enriched particulates from Dunaliella salina.
abstract: In this study, supercritical anti-solvent (SAS) pulverization coupled with reverse phase elution chromatography was employed to isolate 9-cis and trans-β-carotenes from Dunaliella salina. Total concentration of 9-cis (134.7mg/g) and trans-β-carotene (204.2mg/g) was increased from 338.9mg/g of the ultrasonic extract to 859.7mg/g (338.9 for 9-cis and 520.8 for trans) of the elution fraction. The SAS pulverization of the collected fraction further produced submicron-sized particulates containing 932.1mg/g (355.6 for 9-cis and 576.5 for trans) of total β-carotenes with a recovery of 86.3% (83.9% for cis and 87.8% for trans). Effects of two SAS operational conditions on the purity, recovery of total β-carotenes, mean size and morphology of the precipitates were obtained from an experimentally designed method. Generation of micronized particulates enriched with 9-cis and trans-β-carotenes by low-density SAS was proved to be feasible and environmental benign.
authors.affiliation: National Chung Hsing University, Chemical Engineering Department, No. 250, Kuokuang Road, Taichung 402, Taiwan.
authors.forename: Jian-Ren
authors.initials: JR
authors.lastname: Chen
authors.forename: Jia-Jiuan
authors.initials: JJ
authors.lastname: Wu
authors.forename: Justin Chun-Te
authors.initials: JC
authors.lastname: Lin
authors.forename: Yuan-Chuen
authors.initials: YC
authors.lastname: Wang
authors.forename: Chiu-Chung
authors.initials: CC
authors.lastname: Young
authors.forename: Chwen-Jen
authors.initials: CJ
authors.lastname: Shieh
authors.forename: Shih-Lan
authors.initials: SL
authors.lastname: Hsu
authors.forename: Cheih-Ming J
authors.initials: CM
authors.lastname: Chang
mesh_terms: D011232:Chemical Precipitation; D000460:Chlorophyta / Q000737:chemistry*; D002845:Chromatography / Q000379:methods*; D025924:Chromatography, Supercritical Fluid; D019207:beta Carotene / Q000737:chemistry*
chemical_list: D019207:beta Carotene