title: Label-free impedimetric immunosensor for sensitive detection of fenvalerate in tea.
abstract: In this experiment, fenvalerate antibodies were immobilised on the electrode by the crosslinking with glutaraldehyde modified on the glassy carbon electrode (GCE) via chitosan. Fenvalerate was measured by the increase of electron transfer resistance when the immune reaction occurred with Fe(CN)6(3-)/Fe(CN)6(4-) as the probe. Under optimal conditions, the change of resistance is in a linear relationship with the logarithm of the concentration in the range of 1.0×10(-3)∼1.0×10(1)mg/L (R=0.998) with a detection limit of 0.80 μg/L. This method bears such merits as simplicity of operation, high sensitivity, wide linear range, specificity, reproducibility and good stability. The immunosensor was applied in the detection of real samples of tea, achieving satisfactory results, and it could be regenerated after being placed alternately in 0.5 mol/L HCl and 0.5 mol/L NaOH solutions.
authors.affiliation: College of Chemistry and Chemical Engineering, Jiangsu Key Laboratory of Environmental Engineering and Monitoring, Yangzhou University, 180 Si-Wang-Ting Road, Yangzhou 225002, China.
authors.forename: Meirong
authors.initials: M
authors.lastname: Wang
authors.forename: Huimin
authors.initials: H
authors.lastname: Kang
authors.forename: Dan
authors.initials: D
authors.lastname: Xu
authors.forename: Chengyin
authors.initials: C
authors.lastname: Wang
authors.forename: Shuzhao
authors.initials: S
authors.lastname: Liu
authors.forename: Xiaoya
authors.initials: X
authors.lastname: Hu
mesh_terms: D004563:Electrochemistry / Q000295:instrumentation / Q000379:methods*; D005506:Food Contamination / Q000032:analysis; D007118:Immunoassay / Q000295:instrumentation / Q000379:methods*; D007306:Insecticides / Q000032:analysis*; D057230:Limit of Detection; D009570:Nitriles / Q000032:analysis*; D011722:Pyrethrins / Q000032:analysis*; D013662:Tea / Q000737:chemistry*
chemical_list: D007306:Insecticides; D009570:Nitriles; D011722:Pyrethrins; D013662:Tea; C017690:fenvalerate