title: Let7a inhibits the growth of endometrial carcinoma cells by targeting Aurora-B.
abstract: MicroRNAs negatively regulate target gene expression at the post-transcriptional level during carcinogenesis. Recent advances revealed that the expression levels of several miRNAs are up- or down-regulated in endometrial carcinoma (EC). Here we identify dysregulated miRNAs in EC and we elucidate the essential role of let-7a. The expression of 86 miRNAs in EC was found to be different from adjacent normal endometrial tissues. Moreover, miR-let-7 members are down-regulated in EC and let-7 miRNAs are highly associated with endometrial cancer. A functional investigation revealed that let-7a suppressed proliferation of HeLa cells by targeting Aurora-B. Let-7a also antagonizes Aurora-B functions in promoting carcinoma cell proliferation by down-regulating Aurora-B protein level. Let-7a could be applied for gene therapy against endometrial carcinogenesis.
authors.affiliation: Gynecology Department, Changning Maternity and Infant Health Hospital, Shanghai, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Liu
authors.forename: Meiyan
authors.initials: M
authors.lastname: Qi
authors.forename: Chengbin
authors.initials: C
authors.lastname: Ma
authors.forename: Guoying
authors.initials: G
authors.lastname: Lao
authors.forename: Yu
authors.initials: Y
authors.lastname: Liu
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.forename: Yingzi
authors.initials: Y
authors.lastname: Liu
mesh_terms: D064107:Aurora Kinase B; D064127:Aurora Kinases; D049109:Cell Proliferation; D002470:Cell Survival; D016889:Endometrial Neoplasms / Q000235:genetics / Q000473:pathology*; D004717:Endometrium / Q000201:enzymology*; D005260:Female; D020869:Gene Expression Profiling; D015971:Gene Expression Regulation, Enzymologic; D015972:Gene Expression Regulation, Neoplastic; D006367:HeLa Cells; D006801:Humans; D035683:MicroRNAs / Q000235:genetics / Q000502:physiology*; D020411:Oligonucleotide Array Sequence Analysis; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism*
chemical_list: D035683:MicroRNAs; C487931:mirnlet7 microRNA, human; C576921:AURKB protein, human; D064107:Aurora Kinase B; D064127:Aurora Kinases; D017346:Protein Serine-Threonine Kinases
keywords: Endometrial carcinoma cell; Let-7a; MicroRNA; Targeting Aurora-B