title: Extending the range of supercritical fluid chromatography by use of water-rich modifiers.
abstract: In this study we investigate the recently reported use of water-containing modifiers for separation and purification of hydrophilic compounds by supercritical fluid chromatography. Improved peak shape is obtained for a variety of glycosides and otherwise hydrophilic compounds when 5% water is added to the methanol co-solvent used in SFC separations, and examples of the use of this approach in preparative SFC purifications are presented.
authors.affiliation: Merck Research Laboratories, Rahway, New Jersey 07065, USA. jinchu_liu@merck.com
authors.forename: Jinchu
authors.initials: J
authors.lastname: Liu
authors.forename: Erik L
authors.initials: EL
authors.lastname: Regalado
authors.forename: Ingrid
authors.initials: I
authors.lastname: Mergelsberg
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Welch
mesh_terms: D025924:Chromatography, Supercritical Fluid; D006027:Glycosides / Q000302:isolation & purification*; D057927:Hydrophobic and Hydrophilic Interactions; D015394:Molecular Structure; D014867:Water / Q000737:chemistry*
chemical_list: D006027:Glycosides; D014867:Water