title: Recent technological developments in proteomics shed new light on translational research on diabetic microangiopathy.
abstract: Diabetic microangiopathy has become a heavy social burden worldwide, but at present it is still difficult to predict and diagnose this ailment at an early stage. Various proteomics approaches have been applied to the pathophysiological study of diabetic microangiopathy. Conventional proteomics methods, including gel-based methods, exhibit limited sensitivity and robustness and have typically been used in high- or middle-abundance biomarker discovery. Clinical samples from patients with diabetic microangiopathy, such as biopsy samples, are minute in size. Therefore sample preparation, quantitative labelling and mass spectrometry technologies need to be optimized for low-abundance protein detection, multiple-sample processing and precision quantitation. In this review, we briefly introduce the recent technological developments in proteomics methods and summarize current proteomics-based, translational research on diabetic microangiopathy. Recent technological developments in proteomics tools may shed new light on the pathogenesis of diabetic microangiopathy and biomarkers and therapeutic targets related to this condition.
authors.affiliation: Department of Endocrinology and Metabolism, Shanghai First People's Hospital, Shanghai Jiao Tong University, Shanghai, China.
authors.forename: Yuhang
authors.initials: Y
authors.lastname: Ma
authors.forename: Cheng
authors.initials: C
authors.lastname: Yang
authors.forename: Yimin
authors.initials: Y
authors.lastname: Tao
authors.forename: Hu
authors.initials: H
authors.lastname: Zhou
authors.forename: Yufan
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D003925:Diabetic Angiopathies / Q000209:etiology / Q000378:metabolism*; D003928:Diabetic Nephropathies / Q000378:metabolism; D003930:Diabetic Retinopathy / Q000378:metabolism; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods; D040901:Proteomics / Q000379:methods* / Q000639:trends; D057170:Translational Research, Biomedical / Q000379:methods / Q000639:trends
chemical_list: D015415:Biomarkers
keywords: clinical application; diabetic nephropathy; diabetic retinopathy; proteomics; translational research