title: Hyaluronidases and their inhibitors in the serum of colorectal carcinoma patients.
abstract: Colorectal cancer is the third most commonly diagnosed type of cancer. Hyaluronan is involved in this malignancy. Moreover, hyaluronidases - its degrading enzymes - display controversial roles regarding their involvement in tumor development. HYAL-1 is the major tumor derived hyaluronidase. The aim of the study was the determination and evaluation of hyaluronidase levels in serum of colorectal cancer patients, before and after surgery, with a view to assessing its potential role as a tumor marker for recurrence. By zymography and Western blotting, it was confirmed that HYAL-1 was the only hyaluronidase present in samples. Quantification of its activity indicated a statistically significant decrease in samples seven days postoperatively, compared with the respective ones before surgery. HYAL-1 levels before surgery were significantly reduced in comparison with healthy samples and samples one year postoperatively. Hyaluronidase inhibitor activity was demonstrated under mild alkaline conditions via reverse zymography. A statistically significant increase was observed in samples seven days postoperatively, when compared with samples before surgery. HYAL-1 levels in sera of colorectal cancer patients were lower than those of healthy population, possibly because of the local accumulation of the enzyme in tumor microenvironment. A gradual elevation up to one year postoperatively to reach healthy levels might indicate a role of HYAL-1 levels in cancer.
authors.affiliation: Laboratory of Biochemistry, Department of Chemistry, University of Patras, 26500 Patras, Greece.
authors.forename: C
authors.initials: C
authors.lastname: Kolliopoulos
authors.forename: D
authors.initials: D
authors.lastname: Bounias
authors.forename: H
authors.initials: H
authors.lastname: Bouga
authors.forename: D
authors.initials: D
authors.lastname: Kyriakopoulou
authors.forename: M
authors.initials: M
authors.lastname: Stavropoulos
authors.forename: D H
authors.initials: DH
authors.lastname: Vynios
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000097:blood; D015179:Colorectal Neoplasms / Q000097:blood*; D004791:Enzyme Inhibitors / Q000097:blood*; D005260:Female; D006801:Humans; D006821:Hyaluronoglucosaminidase / Q000037:antagonists & inhibitors* / Q000097:blood*; D008297:Male; D008875:Middle Aged
chemical_list: D014408:Biomarkers, Tumor; D004791:Enzyme Inhibitors; D006821:Hyaluronoglucosaminidase