title: 3M Tecra Listeria Visual Immunoassay: AOAC Official Methods 995.22 and 2002.09.
abstract: A validation study of the 3M Tecra Listeria Visual Immunoassay (VIA; 3M Food Safety, St. Paul, MN) was conducted at Q Laboratories, Inc., Cincinnati, OH. The 3M Tecra Listeria VIA method was compared to the Health Canada MFHPB-30 reference method for the analysis of five ready-to-eat (RTE) meats: deli turkey, hot dogs, liver pate, raw fermented sausage, and deli ham, and on a stainless steel environmental surface. Twenty replicates of each of the five food matrixes were analyzed at a low and a high inoculum level. The low-level test portions were inoculated with 0.2-2 CFU/25 g, and the high-level test portions with 2-5 CFU/25 g. In addition, 20 replicates of one environmental surface were analyzed at a low and a high inoculum level. The low-level sampling area was inoculated with 0.2-2 CFU/5 cm2, and the high-level area with 2-5 CFU/5 cm2. Five control replicates were also analyzed at 0 CFU/25 g (uninoculated) for the foods and at 0 CFU/5 cm2 for the environmental sampling area. There was no significant difference in the number of positives detected by the 3M Tecra Listeria VIA and the Health Canada MFHPB-30 reference method for four of the RTE meats and the stainless steel environmental surface analyzed in this study. For the raw, fermented sausage, there was a significant difference in the number of positives detected for the high inoculum level by the 3M Tecra Listeria VIA and the Health Canada MFHPB-30 reference method, with the 3M Tecra Listeria VIA method detecting more positives.
authors.affiliation: 3M Food Safety, 3M Center, Bldg 260-6B-01, Saint Paul, MN 55144-1000, USA. dbenesh1@mmm.com
authors.forename: Deann L
authors.initials: DL
authors.lastname: Benesh
authors.forename: Erin S
authors.initials: ES
authors.lastname: Crowley
authors.forename: Patrick M
authors.initials: PM
authors.lastname: Bird
mesh_terms: D000818:Animals; D004783:Environmental Microbiology / Q000592:standards*; D005516:Food Microbiology / Q000592:standards*; D007118:Immunoassay / Q000379:methods* / Q000592:standards*; D008087:Listeria / Q000302:isolation & purification*; D008460:Meat / Q000382:microbiology; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity