title: Occurrence of plasmid-mediated quinolone resistance determinants and rmtB gene in Salmonella enterica serovar enteritidis and Typhimurium isolated from food-animal products in Tunisia.
abstract: Four hundred and thirty Salmonella isolates, recovered from various food-animal products, were tested for nalidixic acid resistance, plasmid-mediated quinolone resistance, and genetic relationship. One hundred fifteen isolates (113 Salmonella serovar Enteritidis and two Salmonella serovar Typhimurium isolates) of 430 (26.7%) Salmonella isolates exhibited nalidixic acid resistance. Polymerase chain reaction screening for qnrA, qnrB, qnrS, qepA (encoding fluoroquinolones resistance) and rmtB (encoding aminoglycosides resistance) showed that 5 (1.16%) isolates were positive for qnr- and qepA-type genes, and the aac(6')-Ib-cr gene was observed in two (1.7%) Enteritidis isolates concomitantly with qnrA or qnrB. The co-occurrence of qepA and rmtB in one Typhimurium isolate is noteworthy. Pulsed-field gel electrophoresis revealed a high genetic homogeneity of nalidixic-resistant isolates and the persistence of clonal clusters over 4 years in different regions in Tunisia and from various food-animal products. To the best of our knowledge, this is the first report of co-occurrence of qepA and rmtB in a Salmonella strain.
authors.affiliation: Laboratoire de Contrôle des Eaux et Denrées Alimentaires, Centre National de Salmonella, Shigella, et Vibrio spp-Institut Pasteur de Tunis, Tunis, Tunisia.
authors.forename: Nazek
authors.initials: N
authors.lastname: Al-Gallas
authors.forename: Mohamed Salah
authors.initials: MS
authors.lastname: Abbassi
authors.forename: Becher
authors.initials: B
authors.lastname: Gharbi
authors.forename: Molka
authors.initials: M
authors.lastname: Manai
authors.forename: Mohamed N
authors.initials: MN
authors.lastname: Ben Fayala
authors.forename: Raghda
authors.initials: R
authors.lastname: Bichihi
authors.forename: Amna
authors.initials: A
authors.lastname: Al-Gallas
authors.forename: Ridha
authors.initials: R
authors.lastname: Ben Aissa
mesh_terms: D000617:Aminoglycosides / Q000494:pharmacology; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D004269:DNA, Bacterial / Q000235:genetics; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics*; D016521:Electrophoresis, Gel, Pulsed-Field; D024841:Fluoroquinolones / Q000494:pharmacology*; D005506:Food Contamination; D005516:Food Microbiology; D005798:Genes, Bacterial; D008460:Meat / Q000382:microbiology; D008826:Microbial Sensitivity Tests; D008892:Milk / Q000382:microbiology; D012189:Retrospective Studies; D012477:Salmonella enteritidis / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D012486:Salmonella typhimurium / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D014416:Tunisia
chemical_list: D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D024841:Fluoroquinolones