title: Toxicity assessment of inorganic nanoparticles to acetoclastic and hydrogenotrophic methanogenic activity in anaerobic granular sludge.
abstract: Release of engineered nanoparticles (NPs) to municipal wastewater from industrial and residential sources could impact biological systems in wastewater treatment plants. Methanogenic inhibition can cause failure of anaerobic waste(water) treatment. This study investigated the inhibitory effect of a wide array of inorganic NPs (Ag(0), Al₂O₃, CeO₂, Cu(0), CuO, Fe(0), Fe₂O₃, Mn₂O₃, SiO₂, TiO₂, and ZnO supplied up to 1500 mgL(-1)) to acetoclastic and hydrogenotrophic methanogenic activity of anaerobic granular sludge. Of all the NPs tested, only Cu(0) and ZnO caused severe methanogenic inhibition. The 50% inhibiting concentrations determined towards acetoclastic and hydrogenotrophic methanogens were 62 and 68 mgL(-1) for Cu(0) NP; and 87 and 250 mgL(-1) for ZnO NP, respectively. CuO NPs also caused inhibition of acetoclastic methanogens. Cu(2+) and Zn(2+) salts caused similar levels of inhibition as Cu(0) and ZnO NPs based on equilibrium soluble metal concentrations measured during the assays, suggesting that the toxicity was due to the release of metal ions by NP-corrosion. A commercial dispersant, Dispex, intended to increase NP stability did not affect the inhibitory impact of the NPs. The results taken as a whole suggest that Zn- and Cu-containing NPs can release metal ions that are inhibitory for methanogenesis.
authors.affiliation: Department of Chemical and Environmental Engineering, University of Arizona, PO Box 210011, Tucson, AZ 85721, USA. jorgegonzaleze@arizona.edu
authors.forename: Jorge
authors.initials: J
authors.lastname: Gonzalez-Estrella
authors.forename: Reyes
authors.initials: R
authors.lastname: Sierra-Alvarez
authors.forename: James A
authors.initials: JA
authors.lastname: Field
mesh_terms: D001419:Bacteria / Q000187:drug effects / Q000378:metabolism; D001681:Biological Assay; D057919:Biological Oxygen Demand Analysis; D003300:Copper / Q000737:chemistry*; D003343:Corrosion; D007220:Industrial Waste; D007477:Ions; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000633:toxicity; D008697:Methane / Q000032:analysis* / Q000737:chemistry; D010100:Oxygen / Q000737:chemistry; D012722:Sewage / Q000737:chemistry* / Q000382:microbiology; D012995:Solubility; D018675:Toxicity Tests; D014865:Waste Disposal, Fluid / Q000379:methods; D062065:Wastewater; D014874:Water Pollutants, Chemical / Q000032:analysis*; D015034:Zinc Oxide / Q000737:chemistry*
chemical_list: D003300:Copper; D007220:Industrial Waste; D007477:Ions; D008697:Methane; D010100:Oxygen; D012722:Sewage; D062065:Wastewater; D014874:Water Pollutants, Chemical; D015034:Zinc Oxide
keywords: COD; Copper; Engineered nanoparticles; Inhibition; Methanogenesis; NMA; NPs; PSD; VSS; ZP; Zinc oxide; chemical oxygen demand; engineered nanoparticles; normalized methanogenic activity; particle size distribution; volatile suspended solids; zeta potential