title: Cutting edge: Conditional MHC class II expression reveals a limited role for B cell antigen presentation in primary and secondary CD4 T cell responses.
abstract: The activation, differentiation, and subsequent effector functions of CD4 T cells depend on interactions with a multitude of MHC class II (MHCII)-expressing APCs. To evaluate the individual contribution of various APCs to CD4 T cell function, we have designed a new murine tool for selective in vivo expression of MHCII in subsets of APCs. Conditional expression of MHCII in B cells was achieved using a cre-loxP approach. After i.v. or s.c. priming, partial proliferation and activation of CD4 T cells was observed in mice expressing MHCII only by B cells. Restricting MHCII expression to B cells constrained secondary CD4 T cell responses in vivo, as demonstrated in a CD4 T cell-dependent model of autoimmunity, experimental autoimmune encephalomyelitis. These results highlight the limitations of B cell Ag presentation during initiation and propagation of CD4 T cell function in vivo using a novel system to study individual APCs by the conditional expression of MHCII.
authors.affiliation: Department of Neurology, Washington University School of Medicine, St Louis, MO 63110, USA.
authors.forename: Angela S
authors.initials: AS
authors.lastname: Archambault
authors.forename: Javier A
authors.initials: JA
authors.lastname: Carrero
authors.forename: Lisa G
authors.initials: LG
authors.lastname: Barnett
authors.forename: Nigel G
authors.initials: NG
authors.lastname: McGee
authors.forename: Julia
authors.initials: J
authors.lastname: Sim
authors.forename: Jonathan O
authors.initials: JO
authors.lastname: Wright
authors.forename: Tobias
authors.initials: T
authors.lastname: Raabe
authors.forename: Peiquin
authors.initials: P
authors.lastname: Chen
authors.forename: Hua
authors.initials: H
authors.lastname: Ding
authors.forename: Eric J
authors.initials: EJ
authors.lastname: Allenspach
authors.forename: Ioannis
authors.initials: I
authors.lastname: Dragatsis
authors.forename: Terri M
authors.initials: TM
authors.lastname: Laufer
authors.forename: Gregory F
authors.initials: GF
authors.lastname: Wu
mesh_terms: D000818:Animals; D017951:Antigen Presentation; D000938:Antigen-Presenting Cells / Q000276:immunology / Q000378:metabolism; D001402:B-Lymphocytes / Q000276:immunology* / Q000378:metabolism*; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D002460:Cell Line; D049109:Cell Proliferation; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000276:immunology; D000949:Histocompatibility Antigens Class II / Q000276:immunology / Q000378:metabolism*; D008213:Lymphocyte Activation; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout
chemical_list: D000949:Histocompatibility Antigens Class II