title: [Effect of PRP on the proliferation of dermal papilla cells and hair follicle regeneration in mice].
abstract: OBJECTIVE
  To investigate the effects of platelet-rich plasma (PRP) on the proliferation of dermal papilla cells (DPCs) and hair follicle regeneration.
  
  
  METHODS
  PRP was prepared using the double-spin method and applied to DPCs. The proliferative effect of activated PRP on DPCs was measured using MTT assay. To understand the influence of activated PRP on the hair-inductive capacity of DPCs, freshly isolated epidermal cells and DPCs of passage 4 were resuspended, mixed with various concentrations of a PRP (0%, 5% or 10%) and were then transferred to a grafting chamber, which was implanted onto the dorsal skin of nude mice. The chambers were removed 1 week after grafting and HF formation was monitored for 4 weeks; the graft site was harvested and processed for histological examination.
  
  
  RESULTS
  Activated PRP increased the proliferation benefited the aggregative growth of DPCs. There are significant difference in the yield of hair follicles compared with 10% PRP (344 +/- 27) with 0% PRP (288 +/- 35) in the area of reconstituted skin (P < 0.05). The areas treated with PRP demonstrated an increase in hair follicles density of 19.4%. Ten percent PRP (18 +/- 1) d also can significantly shorten the time of hair formation, compared with 0% PRP (20 +/- 1) d (P < 0.05).
  
  
  CONCLUSIONS
  There is a considerable effect of PRP on the time of hair formation and the yield of hair follicles reconstitution.
authors.affiliation: Department of Plastic and Reconstructive Surgery, Nan Fang Hospital of Southern Medical University, Guangzhou 510515, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Miao
authors.forename: Chuan-Bo
authors.initials: CB
authors.lastname: Feng
authors.forename: Zhi-Dan
authors.initials: ZD
authors.lastname: Zhang
authors.forename: Ze-Hua
authors.initials: ZH
authors.lastname: Li
authors.forename: Shun-E
authors.initials: SE
authors.lastname: Xiao
authors.forename: Jin-Dou
authors.initials: JD
authors.lastname: Jiang
authors.forename: Zhi-Qi
authors.initials: ZQ
authors.lastname: Hu
mesh_terms: D000818:Animals; D049109:Cell Proliferation; D002478:Cells, Cultured; D005260:Female; D018859:Hair Follicle / Q000166:cytology / Q000254:growth & development*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008819:Mice, Nude; D053657:Platelet-Rich Plasma; D012038:Regeneration; D012867:Skin / Q000166:cytology*; D017768:Skin, Artificial