title: Human leukocyte antigen (HLA) typing by DNA sequencing.
abstract: DNA sequencing is a powerful technique for identifying allelic variation within the human leukocyte antigen (HLA) genes. Sequencing is usually focused on the most polymorphic exons of the class I (HLA-A, -B, -C) and class II (HLA-DR, -DQ, and -DP) genes. These exons encode the antigen recognition site, the region of the HLA molecule that binds peptides and interacts with the T cell receptor for antigen and natural killer cell immunoglobulin-like receptors (KIR). Sanger sequencing of amplified DNA from each HLA gene from a preparation containing one or two alleles yields a sequence that is used to identify the alleles by comparison with a reference database.
authors.affiliation: CW Bill Young Marrow Donor Recruitment and Research Program, Department of Pediatrics, Georgetown University Medical Center, Rockville, MD, USA.
authors.forename: Ana
authors.initials: A
authors.lastname: Lazaro
authors.forename: Bin
authors.initials: B
authors.lastname: Tu
authors.forename: Ruyan
authors.initials: R
authors.lastname: Yang
authors.forename: Yi
authors.initials: Y
authors.lastname: Xiao
authors.forename: Kanthi
authors.initials: K
authors.lastname: Kariyawasam
authors.forename: Jennifer
authors.initials: J
authors.lastname: Ng
authors.forename: Carolyn Katovich
authors.initials: CK
authors.lastname: Hurley
mesh_terms: D000483:Alleles; D005091:Exons; D005838:Genotype; D006682:HLA-DP Antigens / Q000235:genetics / Q000302:isolation & purification; D006683:HLA-DQ Antigens / Q000235:genetics / Q000302:isolation & purification; D006684:HLA-DR Antigens / Q000235:genetics / Q000302:isolation & purification; D000949:Histocompatibility Antigens Class II / Q000145:classification* / Q000235:genetics; D006650:Histocompatibility Testing / Q000379:methods*; D006801:Humans; D008967:Molecular Biology / Q000379:methods*; D054340:Receptors, KIR / Q000235:genetics / Q000276:immunology; D055607:Receptors, Natural Killer Cell / Q000145:classification / Q000235:genetics / Q000276:immunology; D017422:Sequence Analysis, DNA
chemical_list: D006682:HLA-DP Antigens; D006683:HLA-DQ Antigens; D006684:HLA-DR Antigens; D000949:Histocompatibility Antigens Class II; D054340:Receptors, KIR; D055607:Receptors, Natural Killer Cell