title: DNA capture-probe based separation of double-stranded polymerase chain reaction amplification products in poly(dimethylsiloxane) microfluidic channels.
abstract: Herein, we describe the development of a novel primer system that allows for the capture of double-stranded polymerase chain reaction (PCR) amplification products onto a microfluidic channel without any preliminary purification stages. We show that specially designed PCR primers consisting of the main primer sequence and an additional "tag sequence" linked through a poly(ethylene glycol) molecule can be used to generate ds-PCR amplification products tailed with ss-oligonucleotides of two forensically relevant genes (amelogenin and human c-fms (macrophage colony-stimulating factor) proto-oncogene for the CSF-1 receptor (CSF1PO). Furthermore, with a view to enriching and eluting the ds-PCR products of amplification on a capillary electrophoretic-based microfluidic device we describe the capture of the target ds-PCR products onto poly(dimethylsiloxane) microchannels modified with ss-oligonucleotide capture probes.
authors.affiliation: Flinders Centre for NanoScale Science and Technology, School of Chemical and Physical Sciences, Flinders University, GPO Box 2100, Adelaide, SA 5001, Australia.
authors.forename: Dmitriy
authors.initials: D
authors.lastname: Khodakov
authors.forename: Leigh
authors.initials: L
authors.lastname: Thredgold
authors.forename: Claire E
authors.initials: CE
authors.lastname: Lenehan
authors.forename: Gunther G
authors.initials: GG
authors.lastname: Andersson
authors.forename: Hilton
authors.initials: H
authors.lastname: Kobus
authors.forename: Amanda V
authors.initials: AV
authors.lastname: Ellis