title: 3D structural patterns in scalable, elastomeric scaffolds guide engineered tissue architecture.
abstract: Microfabricated elastomeric scaffolds with 3D structural patterns are created by semiautomated layer-by-layer assembly of planar polymer sheets with through-pores. The mesoscale interconnected pore architectures governed by the relative alignment of layers are shown to direct cell and muscle-like fiber orientation in both skeletal and cardiac muscle, enabling scale up of tissue constructs towards clinically relevant dimensions.
authors.affiliation: Harvard-MIT Division of Health Sciences and Technology, David H. Koch Institute for Integrative Cancer Research, Institute for Medical Engineering and Science, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
authors.forename: Martin E
authors.initials: ME
authors.lastname: Kolewe
authors.forename: Hyoungshin
authors.initials: H
authors.lastname: Park
authors.forename: Caprice
authors.initials: C
authors.lastname: Gray
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Ye
authors.forename: Robert
authors.initials: R
authors.lastname: Langer
authors.forename: Lisa E
authors.initials: LE
authors.lastname: Freed
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002460:Cell Line; D003651:Decanoates / Q000737:chemistry*; D020724:Elastomers; D005990:Glycerol / Q000031:analogs & derivatives* / Q000737:chemistry; D051379:Mice; D055616:Microtechnology; D032446:Myoblasts / Q000166:cytology*; D009206:Myocardium / Q000166:cytology*; D011108:Polymers / Q000737:chemistry*; D016062:Porosity; D051381:Rats; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D003651:Decanoates; D020724:Elastomers; D011108:Polymers; C036925:elastomeric polymer; C469892:poly(glycerol-sebacate); D005990:Glycerol
keywords: alignment; heart; microfabrication; muscle; polyglycerol sebacate (PGS)