title: Sulforaphane protects H9c2 cardiomyocytes from angiotensin II-induced hypertrophy.
abstract: BACKGROUND
  Cardiac hypertrophy is an adaptive process of the heart in response to various stimuli, but sustained cardiac hypertrophy will finally lead to heart failure. Sulforaphane-extracted from cruciferous vegetables of the genus Brassica such as broccoli, brussels sprouts, and cabbage-has been evaluated for its anticarcinogenic and antioxidant effects.
  
  
  OBJECTIVE
  To investigate the effect of sulforaphane on angiotensin II (Ang II)-induced cardiac hypertrophy in vitro.
  
  
  METHODS
  Embryonic rat heart-derived H9c2 cells were co-incubated with sulforaphane and Ang II. The cell surface area and mRNA levels of hypertrophic markers were measured to clarify the effect of sulforaphane on cardiac hypertrophy. The underlying mechanism was further investigated by detecting the activation of Akt and NF-κB signaling pathways.
  
  
  RESULTS
  We found that H9c2 cells pretreated with sulforaphane were protected from Ang II-induced hypertrophy. The increasing mRNA levels of ANP, BNP, and β-MHC in Ang II-stimulated cells were also down-regulated after sulforaphane treatment. Moreover, sulforaphane repressed the Ang II-induced phosphorylation of Akt, GSK3β, mTOR, eIF4e, as well as of IκBα and NF-κB.
  
  
  CONCLUSIONS
  Based on our results, sulforaphane attenuates Ang II-induced hypertrophy of H9c2 cardiomyocytes mediated by the inhibition of intracellular signaling pathways including Akt and NF-κB.
authors.affiliation: Department of Cardiology, Renmin Hospital of Wuhan University, JieFang Road 238, 430060, Wuhan, China.
authors.forename: Q-Q
authors.initials: QQ
authors.lastname: Wu
authors.forename: J
authors.initials: J
authors.lastname: Zong
authors.forename: L
authors.initials: L
authors.lastname: Gao
authors.forename: J
authors.initials: J
authors.lastname: Dai
authors.forename: Z
authors.initials: Z
authors.lastname: Yang
authors.forename: M
authors.initials: M
authors.lastname: Xu
authors.forename: Y
authors.initials: Y
authors.lastname: Fang
authors.forename: Z-G
authors.initials: ZG
authors.lastname: Ma
authors.forename: Q-Z
authors.initials: QZ
authors.lastname: Tang
mesh_terms: D000804:Angiotensin II; D000818:Animals; D002316:Cardiotonic Agents / Q000008:administration & dosage; D002460:Cell Line; D048429:Cell Size / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D017379:Hypertrophy, Left Ventricular / Q000139:chemically induced / Q000378:metabolism* / Q000517:prevention & control*; D017879:Isothiocyanates / Q000008:administration & dosage*; D032383:Myocytes, Cardiac / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology*; D016328:NF-kappa B / Q000378:metabolism*; D051058:Oncogene Protein v-akt / Q000378:metabolism*; D051381:Rats; D013454:Sulfoxides; D016896:Treatment Outcome
chemical_list: D002316:Cardiotonic Agents; D017879:Isothiocyanates; D016328:NF-kappa B; D013454:Sulfoxides; D000804:Angiotensin II; D051058:Oncogene Protein v-akt; C016766:sulforaphane