title: Attenuated Salmonella enterica serovar Typhimurium lacking the ZnuABC transporter: an efficacious orally-administered mucosal vaccine against salmonellosis in pigs.
abstract: We have recently demonstrated that an attenuated strain of Salmonella enterica serovar Typhimurium unable to synthesize the zinc transporter ZnuABC (S. Typhimurium ΔznuABC), is able to protect mice against systemic and enteric salmonellosis and is safe in pigs. Here, we have tested the protective effects of S. Typhimurium ΔznuABC in pigs. Resistance to challenge with the fully virulent strain S. Typhimurium ATCC 14028 was assessed in animals vaccinated with S. Typhimurium ΔznuABC (two dosages tested), in controls vaccinated with a formalin-inactivated virulent strain and in unvaccinated controls. Clinical signs of salmonellosis, faecal shedding and bacterial colonization of organs were used to assess vaccine-induced protection. After the challenge, pigs vaccinated with the attenuated S. Typhimurium ΔznuABC strain did not display clinical signs of salmonellosis (fever or diarrhoea). The vaccine also reduced intestinal tract colonization and faecal shedding of the fully virulent Salmonella strain, as compared to control groups. S. Typhimurium ΔznuABC represents a promising candidate vaccine against salmonellosis in pigs.
authors.affiliation: Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna, 25124 Brescia, Italy.
authors.forename: Matteo
authors.initials: M
authors.lastname: Gradassi
authors.forename: Michele
authors.initials: M
authors.lastname: Pesciaroli
authors.forename: Nicola
authors.initials: N
authors.lastname: Martinelli
authors.forename: Jessica
authors.initials: J
authors.lastname: Ruggeri
authors.forename: Paola
authors.initials: P
authors.lastname: Petrucci
authors.forename: Walid Hamdy
authors.initials: WH
authors.lastname: Hassan
authors.forename: Manuela
authors.initials: M
authors.lastname: Raffatellu
authors.forename: Frine Eleonora
authors.initials: FE
authors.lastname: Scaglione
authors.forename: Serena
authors.initials: S
authors.lastname: Ammendola
authors.forename: Andrea
authors.initials: A
authors.lastname: Battistoni
authors.forename: Giovanni L
authors.initials: GL
authors.lastname: Alborali
authors.forename: Paolo
authors.initials: P
authors.lastname: Pasquali
mesh_terms: D000284:Administration, Oral; D000818:Animals; D001426:Bacterial Proteins / Q000008:administration & dosage / Q000276:immunology*; D057138:Bacterial Shedding; D027682:Cation Transport Proteins / Q000008:administration & dosage / Q000276:immunology*; D005243:Feces / Q000382:microbiology; D005260:Female; D018928:Immunity, Mucosal; D007371:Interferon-gamma / Q000276:immunology; D009061:Mouth Mucosa / Q000276:immunology; D012481:Salmonella Infections, Animal / Q000517:prevention & control*; D022562:Salmonella Vaccines / Q000008:administration & dosage / Q000276:immunology*; D012486:Salmonella typhimurium / Q000276:immunology / Q000472:pathogenicity; D034421:Sus scrofa; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology; D014613:Vaccines, Attenuated / Q000008:administration & dosage / Q000276:immunology; D014774:Virulence
chemical_list: D001426:Bacterial Proteins; D027682:Cation Transport Proteins; D022562:Salmonella Vaccines; D014409:Tumor Necrosis Factor-alpha; D014613:Vaccines, Attenuated; D007371:Interferon-gamma
keywords: Mucosal vaccine; Oral delivery; Pigs; S. Typhimurium ΔznuABC; Salmonella; Salmonellosis; Vaccine