title: Ultra-small and anionic starch nanospheres: formation and vitro thrombolytic behavior study.
abstract: This paper is considered as the first report on the investigation of nattokinase (NK) release from anionic starch nanospheres. The ultra-small and anionic starch nanospheres were prepared by the method of reverse micro-emulsion crosslinking in this work. Starch nanospheres were characterized through Fourier transform infrared (FTIR) spectroscopy, scanning electron microscopy (SEM), transmission electron microscopy (TEM) and dynamic light scattering (DLS). Effects of preparation conditions on particle size were studied. The cytotoxicity, biodegradable and vitro thrombolytic behaviors of nattokinase (NK) loaded anionic starch nanospheres were also studied. The results showed that the anionic starch nanospheres are non-toxic, biocompatible and biodegradable. Moreover, the anionic starch nanospheres can protect NK from fast biodegradation hence prolongs the circulation in vivo and can reduce the risk of acute hemorrhage complication by decreasing the thrombolysis rate.
authors.affiliation: Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province, State Key Laboratory of Applied Organic Chemistry and Department of Chemistry, Lanzhou University, Lanzhou 730000, PR China.
authors.forename: Yinjuan
authors.initials: Y
authors.lastname: Huang
authors.forename: Shenglong
authors.initials: S
authors.lastname: Ding
authors.forename: Mingzhu
authors.initials: M
authors.lastname: Liu
authors.forename: Chunmei
authors.initials: C
authors.lastname: Gao
authors.forename: Jinlong
authors.initials: J
authors.lastname: Yang
authors.forename: Xinjie
authors.initials: X
authors.lastname: Zhang
authors.forename: Bin
authors.initials: B
authors.lastname: Ding
mesh_terms: D000818:Animals; D000838:Anions / Q000737:chemistry; D001777:Blood Coagulation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002645:Chickens; D003603:Cytotoxins / Q000737:chemistry* / Q000494:pharmacology; D005343:Fibrinolytic Agents / Q000737:chemistry* / Q000494:pharmacology; D006367:HeLa Cells; D006801:Humans; D054118:Nanospheres / Q000737:chemistry*; D013213:Starch / Q000737:chemistry*; D013381:Subtilisins / Q000737:chemistry* / Q000494:pharmacology
chemical_list: D000838:Anions; D003603:Cytotoxins; D005343:Fibrinolytic Agents; D013213:Starch; D013381:Subtilisins; C053771:nattokinase