title: Multiple drug resistant, tumorigenic stem-like cells in oral cancer.
abstract: An in vitro cell line model was established to exemplify tumor stem cell concept in oral cancer. We were able to identify CD147 expressing fractions in SCC172 OSCC cell line with differing Hoechst dye efflux activity and DNA content. In vivo tumorigenic assay revealed three fractions enriched with stem-like cells capable of undergoing mesenchymal transition and a non-tumorigenic fraction. The regeneration potential and transition of one fraction to other imitated the phenotypic switch and functional disparities evidenced during oral tumor progression. Knowledge of these additional stem-like subsets will improve understanding of stem cell based oral epithelial tumor progression from normal to malignant lesions.
authors.affiliation: Cancer Research Program, Rajiv Gandhi Centre for Biotechnology, Thiruvananthapuram 695014, Kerala State, India.
authors.forename: Vinitha
authors.initials: V
authors.lastname: Richard
authors.forename: Paul
authors.initials: P
authors.lastname: Sebastian
authors.forename: Madhumathy G
authors.initials: MG
authors.lastname: Nair
authors.forename: Sree Narayanan
authors.initials: SN
authors.lastname: Nair
authors.forename: Tessy Thomas
authors.initials: TT
authors.lastname: Malieckal
authors.forename: T R
authors.initials: TR
authors.lastname: Santhosh Kumar
authors.forename: M Radhakrishna
authors.initials: MR
authors.lastname: Pillai
mesh_terms: D018435:ATP Binding Cassette Transporter, Subfamily B; D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000096:biosynthesis; D000080924:Aldehyde Dehydrogenase 1 Family; D000818:Animals; D051926:Basigin / Q000096:biosynthesis; D014408:Biomarkers, Tumor / Q000096:biosynthesis; D063646:Carcinogenesis; D002294:Carcinoma, Squamous Cell / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology*; D045744:Cell Line, Tumor; D019008:Drug Resistance, Neoplasm; D006258:Head and Neck Neoplasms / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology*; D006801:Humans; D007150:Immunohistochemistry; D039641:Integrin beta Chains / Q000096:biosynthesis; D007527:Isoenzymes / Q000096:biosynthesis; D008297:Male; D008565:Membrane Proteins / Q000096:biosynthesis; D051379:Mice; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D009062:Mouth Neoplasms / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology*; D018365:Neoplasm, Residual; D014411:Neoplastic Stem Cells / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology*; D050697:Retinal Dehydrogenase / Q000096:biosynthesis; D000077195:Squamous Cell Carcinoma of Head and Neck; D023041:Xenograft Model Antitumor Assays
chemical_list: C480744:ABCB5 protein, human; D018435:ATP Binding Cassette Transporter, Subfamily B; D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; C497886:BSG protein, human; D014408:Biomarkers, Tumor; C081876:CKAP4 protein, human; D039641:Integrin beta Chains; D007527:Isoenzymes; D008565:Membrane Proteins; C072170:integrin beta6; D051926:Basigin; D000080924:Aldehyde Dehydrogenase 1 Family; C510223:ALDH1A1 protein, human; C515028:ALDH1A1 protein, mouse; D050697:Retinal Dehydrogenase
keywords: Cancer stem cells; Minimal residual disease; Multi-drug resistance; Oral cancer; Side population; Tumorigenic potential