title: Effects of allicin on hyperhomocysteinemia-induced experimental vascular endothelial dysfunction.
abstract: This study was designed to investigate the effect and mechanism of allicin on hyperhomocysteinemia-induced experimental vascular endothelial dysfunction in rats. Fifty male Wistar rats were randomly divided into five groups: the normal control rats (NC), the high-methionine-diet rats (Met), the high-methionine-diet rats treated with folic acid, vitaminB₆ and vitaminB₁₂ (Met+F), or with low-dose allicin (Met+L), or with high-dose allicin (Met+H). After 6 weeks, we collected blood samples of all groups to determine plasma endothelin (ET), serum homocysteine (Hcy), nitric oxide (NO), superoxide dismutase (SOD), malondialdehyde (MDA), and detected the expression of basic fibroblast growth factors (bFGF), transforming growth factor beta (TGF-β), tumor necrosis factor-alpha (TNF-α), and intercellular adhesion molecule-1 (ICAM-1) in the aorta. The Hcy and the expression of TGF-β in both the Met+L and Met+H groups were significantly lower than the Met and Met+F groups. The ET, ET/NO ratio and the MDA levels of the Met+L and Met+H groups were significantly lower than the Met group. The SOD and NO levels and the expression of bFGF, TNF-α and ICAM-1 of the Met+L and Met+H groups were significantly higher than the Met group. Our data indicate that allicin inhibits lipid peroxidation induced by hyperhomocysteinemia and regulates the excretion and equilibrium of ET and NO, and suggest that allicin might be useful in the prevention of endothelial dysfunction caused by hyperhomocysteinemia.
authors.affiliation: Department of Traditional Chinese Medicine, Qilu Hospital of Shandong University, Jinan 250012, PR China. liudeshan@sdu.edu.cn
authors.forename: De-shan
authors.initials: DS
authors.lastname: Liu
authors.forename: Wei
authors.initials: W
authors.lastname: Gao
authors.forename: Er-shun
authors.initials: ES
authors.lastname: Liang
authors.forename: Shu-li
authors.initials: SL
authors.lastname: Wang
authors.forename: Wei-wei
authors.initials: WW
authors.lastname: Lin
authors.forename: Wei-dong
authors.initials: WD
authors.lastname: Zhang
authors.forename: Qing
authors.initials: Q
authors.lastname: Jia
authors.forename: Rui-chen
authors.initials: RC
authors.lastname: Guo
authors.forename: Ji-dong
authors.initials: JD
authors.lastname: Zhang
mesh_terms: D000818:Animals; D001011:Aorta / Q000187:drug effects / Q000378:metabolism; D004220:Disulfides; D016232:Endothelins / Q000378:metabolism; D004730:Endothelium, Vascular / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects; D006710:Homocysteine / Q000097:blood; D020138:Hyperhomocysteinemia / Q000097:blood / Q000378:metabolism* / Q000473:pathology*; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D009569:Nitric Oxide / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013441:Sulfinic Acids / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000097:blood; D016212:Transforming Growth Factor beta / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D004220:Disulfides; D016232:Endothelins; D013441:Sulfinic Acids; D016212:Transforming Growth Factor beta; D014409:Tumor Necrosis Factor-alpha; D006710:Homocysteine; D016222:Fibroblast Growth Factor 2; D018799:Intercellular Adhesion Molecule-1; D009569:Nitric Oxide; C006452:allicin; D008315:Malondialdehyde; D013482:Superoxide Dismutase
keywords: Allicin; Endothelial function; Homocysteine; Nitric oxide; Oxidative stress