title: Microfluidic chip coupled with modified paramagnetic particles for sarcosine isolation in urine.
abstract: Carcinoma of prostate (CaP) is the second most frequent malignant tumor occurring in men in Europe. Currently there is discussion on a wide range of potential CaP markers.One of them—nonprotein amino acid sarcosine, also known as N-methylglycine was chosen as a challenge for the development of microfluidic system with isolation by modified paramagnetic microparticles. Therefore, the aim of this study was to design a low-cost, simple, and rapid microfluidic system based on sarcosine isolation with modified paramagnetic microparticles and subsequent analysis on the ion exchange LC. We modified Dowex microparticles with Fe2O3 nanoparticles. Our paramagnetic microparticles were able to establish the binding with sarcosine. Moreover, we designed microfluidic device for sarcosine determination. Analysis of samples was carried out with LOD of1 M of a sarcosine that is sufficient because it is similar to concentrations of a sarcosine observed in the CaP patients.Carcinoma of prostate (CaP) is the second most frequent malignant tumor occurring in men in Europe. Currently there is discussion on a wide range of potential CaP markers.One of them—nonprotein amino acid sarcosine, also known as N-methylglycine was chosen as a challenge for the development of microfluidic system with isolation by modified paramagnetic microparticles. Therefore, the aim of this study was to design a low-cost, simple, and rapid microfluidic system based on sarcosine isolation with modified paramagnetic microparticles and subsequent analysis on the ion exchange LC. We modified Dowex microparticles with Fe2O3 nanoparticles. Our paramagnetic microparticles were able to establish the binding with sarcosine. Moreover, we designed microfluidic device for sarcosine determination. Analysis of samples was carried out with LOD of1 M of a sarcosine that is sufficient because it is similar to concentrations of a sarcosine observed in the CaP patients.
authors.forename: Ondrej
authors.initials: O
authors.lastname: Zitka
authors.forename: Natalia
authors.initials: N
authors.lastname: Cernei
authors.forename: Zbynek
authors.initials: Z
authors.lastname: Heger
authors.forename: Miroslav
authors.initials: M
authors.lastname: Matousek
authors.forename: Pavel
authors.initials: P
authors.lastname: Kopel
authors.forename: Jindrich
authors.initials: J
authors.lastname: Kynicky
authors.forename: Michal
authors.initials: M
authors.lastname: Masarik
authors.forename: Rene
authors.initials: R
authors.lastname: Kizek
authors.forename: Vojtech
authors.initials: V
authors.lastname: Adam
mesh_terms: D000328:Adult; D002852:Chromatography, Ion Exchange / Q000379:methods; D052203:Ferrosoferric Oxide / Q000737:chemistry*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008297:Male; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods*; D008863:Microspheres; D008954:Models, Biological; D012521:Sarcosine / Q000737:chemistry / Q000302:isolation & purification / Q000652:urine*; D013696:Temperature
chemical_list: D052203:Ferrosoferric Oxide; D012521:Sarcosine