title: Fork sensing and strand switching control antagonistic activities of RecQ helicases.
abstract: RecQ helicases have essential roles in maintaining genome stability during replication and in controlling double-strand break repair by homologous recombination. Little is known about how the different RecQ helicases found in higher eukaryotes achieve their specialized and partially opposing functions. Here, we investigate the DNA unwinding of RecQ helicases from Arabidopsis thaliana, AtRECQ2 and AtRECQ3 at the single-molecule level using magnetic tweezers. Although AtRECQ2 predominantly unwinds forked DNA substrates in a highly repetitive fashion, AtRECQ3 prefers to rewind, that is, to close preopened DNA forks. For both enzymes, this process is controlled by frequent strand switches and active sensing of the unwinding fork. The relative extent of the strand switches towards unwinding or towards rewinding determines the predominant direction of the enzyme. Our results provide a simple explanation for how different biological activities can be achieved by rather similar members of the RecQ family.
authors.affiliation: Biotechnology Center, Technische Universität Dresden, Dresden 01062, Germany.
authors.forename: Daniel
authors.initials: D
authors.lastname: Klaue
authors.forename: Daniela
authors.initials: D
authors.lastname: Kobbe
authors.forename: Felix
authors.initials: F
authors.lastname: Kemmerich
authors.forename: Alicja
authors.initials: A
authors.lastname: Kozikowska
authors.forename: Holger
authors.initials: H
authors.lastname: Puchta
authors.forename: Ralf
authors.initials: R
authors.lastname: Seidel
mesh_terms: D017360:Arabidopsis / Q000201:enzymology*; D029681:Arabidopsis Proteins / Q000378:metabolism*; D004261:DNA Replication; D018744:DNA, Plant / Q000737:chemistry / Q000378:metabolism; D004277:DNA, Single-Stranded / Q000737:chemistry / Q000378:metabolism; D008954:Models, Biological; D009690:Nucleic Acid Conformation; D053484:RecQ Helicases / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; D018744:DNA, Plant; D004277:DNA, Single-Stranded; D053484:RecQ Helicases