title: Development and validation of a sensitive spectrofluorimetric method for the determination of amoxapine in human plasma and urine.
abstract: A selective and sensitive spectrofluorimetric method was developed and validated for the determination of amoxapine in human plasma and urine. The developed method is based on labeling with 5-dimethylaminonaphthalene-1-sulfonyl chloride (dansyl chloride) and monitoring at 397 nm (excitation)/514 nm (emission). The method was validated for linearity, limit of detection (LOD), limit of quantification (LOQ), precision, accuracy, recovery and robustness. The calibration curves were linear over a concentration range of 250-2500 and 50-1250 ng/mL for plasma and urine, respectively. The LOD values were calculated to be 13.31 and 13.17 ng/mL for plasma and urine, respectively. The proposed method was applied to study of amoxapine in human plasma and urine.
authors.affiliation: Department of Chemistry, Faculty of Science and Letters, Namik Kemal University, Tekirdag, Turkey.
authors.forename: Ayça
authors.initials: A
authors.lastname: Karasakal
authors.forename: Sevgi Tatar
authors.initials: ST
authors.lastname: Ulu
mesh_terms: D000657:Amoxapine / Q000097:blood* / Q000652:urine*; D000928:Antidepressive Agents / Q000097:blood* / Q000652:urine*; D006801:Humans; D057230:Limit of Detection; D008297:Male; D013050:Spectrometry, Fluorescence / Q000379:methods*
chemical_list: D000928:Antidepressive Agents; D000657:Amoxapine
keywords: dansyl chloride; derivatization; plasma; spectrofluorimetry; urine; validation