title: Identification of the grincamycin gene cluster unveils divergent roles for GcnQ in different hosts, tailoring the L-rhodinose moiety.
abstract: The gene cluster responsible for grincamycin (GCN, 1) biosynthesis in Streptomyces lusitanus SCSIO LR32 was identified; heterologous expression of the GCN cluster in S. coelicolor M512 yielded P-1894B (1b) as a predominant product. The ΔgcnQ mutant accumulates intermediate 1a and two shunt products 2a and 3a bearing L-rhodinose for L-cinerulose A substitutions. In vitro data demonstrated that GcnQ is capable of iteratively tailoring the two L-rhodinose moieties into L-aculose moieties, supporting divergent roles of GcnQ in different hosts.
authors.affiliation: CAS Key Laboratory of Marine Bio-resources Sustainable Utilization, RNAM Center for Marine Microbiology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, 164 West Xingang Road, Guangzhou 510301, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Zhang
authors.forename: Hongbo
authors.initials: H
authors.lastname: Huang
authors.forename: Qi
authors.initials: Q
authors.lastname: Chen
authors.forename: Minghe
authors.initials: M
authors.lastname: Luo
authors.forename: Aijun
authors.initials: A
authors.lastname: Sun
authors.forename: Yongxiang
authors.initials: Y
authors.lastname: Song
authors.forename: Junying
authors.initials: J
authors.lastname: Ma
authors.forename: Jianhua
authors.initials: J
authors.lastname: Ju
mesh_terms: D000880:Anthraquinones / Q000737:chemistry* / Q000378:metabolism; D001551:Benz(a)Anthracenes / Q000737:chemistry / Q000378:metabolism; D003001:Cloning, Molecular; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009005:Monosaccharides / Q000737:chemistry* / Q000378:metabolism; D005810:Multigene Family; D019906:Nuclear Magnetic Resonance, Biomolecular; D048372:Streptomyces coelicolor / Q000235:genetics* / Q000378:metabolism*; D000095602:Angucyclines and Angucyclinones
chemical_list: D000880:Anthraquinones; D001551:Benz(a)Anthracenes; D009005:Monosaccharides; C438054:rhodinose; C054853:grincamycin; C103671:tetrangomycin; D000095602:Angucyclines and Angucyclinones; C032958:vineomycin A1