title: Vialinin A is a ubiquitin-specific peptidase inhibitor.
abstract: Vialinin A, a small compound isolated from the Chinese mushroom Thelephora vialis, exhibits more effective anti-inflammatory activity than the widely used immunosuppressive drug tacrolimus (FK506). Here, we show that ubiquitin-specific peptidase 5/isopeptidase T (USP5/IsoT) is a target molecule of vialinin A, identified by using a beads-probe method. Vialinin A inhibited the peptidase activity of USP5/IsoT and also inhibited the enzymatic activities of USP4 among deubiquitinating enzymes tested. Although USPs are a member of thiol protease family, vialinin A exhibited no inhibitions for other thiol proteases, such as calpain and cathepsin.
authors.affiliation: Department of Bioscience, Tokyo University of Agriculture, Tokyo 156-8502, Japan.
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Okada
authors.forename: Yue Qi
authors.initials: YQ
authors.lastname: Ye
authors.forename: Kayoko
authors.initials: K
authors.lastname: Taniguchi
authors.forename: Ayaka
authors.initials: A
authors.lastname: Yoshida
authors.forename: Tomonori
authors.initials: T
authors.lastname: Akiyama
authors.forename: Yasukiyo
authors.initials: Y
authors.lastname: Yoshioka
authors.forename: Jun-ichi
authors.initials: J
authors.lastname: Onose
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Koshino
authors.forename: Shunya
authors.initials: S
authors.lastname: Takahashi
authors.forename: Arata
authors.initials: A
authors.lastname: Yajima
authors.forename: Naoki
authors.initials: N
authors.lastname: Abe
authors.forename: Shunsuke
authors.initials: S
authors.lastname: Yajima
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry* / Q000378:metabolism; D002460:Cell Line; D010450:Endopeptidases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D011480:Protease Inhibitors / Q000737:chemistry* / Q000378:metabolism; D011485:Protein Binding; D051381:Rats; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics; D013730:Terphenyl Compounds / Q000737:chemistry* / Q000378:metabolism
chemical_list: D000893:Anti-Inflammatory Agents; D011480:Protease Inhibitors; D011994:Recombinant Proteins; D013730:Terphenyl Compounds; C508745:vialinin A; D010450:Endopeptidases; C091939:ubiquitin isopeptidase