title: Gluconacetobacter tumulisoli sp. nov., Gluconacetobacter takamatsuzukensis sp. nov. and Gluconacetobacter aggeris sp. nov., isolated from Takamatsuzuka Tumulus samples before and during the dismantling work in 2007.
abstract: Ten strains of Gram-stain-negative, rod-shaped, non-spore-forming bacteria were isolated from the burial mound soil collected before the dismantling and samples collected during the dismantling work on the Takamatsuzuka Tumulus in Asuka village, Nara Prefecture, Japan in 2007. On the basis of the 16S rRNA gene sequence analysis of the isolates, they were accommodated in the genus Gluconacetobacter (class Alphaproteobacteria) and can be separated into four groups within the cluster containing the genus Gluconacetobacter. One of the groups demonstrated a phylogenetic position identical to that of Gluconacetobacter asukensis, which was isolated from small holes on plaster walls of the stone chamber interior of Kitora Tumulus in Asuka village, Nara Prefecture, Japan. The remaining three groups consisted of novel lineages within the genus Gluconacetobacter. A total of four isolates were selected from each group and carefully identified using a polyphasic approach. The isolates were characterized on the basis of their possessing Q-10 as the major ubiquinone system and C18 : 1ω7c (58.5-65.2 %) as the predominant fatty acid. A DNA-DNA hybridization test was used to determine that the three lineages represented novel species, for which the names Gluconacetobacter tumulisoli sp. nov., Gluconacetobacter takamatsuzukensis sp. nov. and Gluconacetobacter aggeris sp. nov. are proposed. The type strains are T611xx-1-4a(T) ( = JCM 19097(T) = NCIMB 14861(T)), T61213-20-1a(T) ( = JCM 19094(T) = NCIMB 14859(T)) and T6203-4-1a(T) ( = JCM 19092(T) = NCIMB 14860(T)), respectively.
authors.affiliation: Technical Department, TechnoSuruga Laboratory Co. Ltd, 330 Nagasaki, Shimizu-ku, Shizuoka-shi, Shizuoka 424-0065, Japan.
authors.forename: Miyuki
authors.initials: M
authors.lastname: Nishijima
authors.affiliation: Technical Department, TechnoSuruga Laboratory Co. Ltd, 330 Nagasaki, Shimizu-ku, Shizuoka-shi, Shizuoka 424-0065, Japan.
authors.forename: Nozomi
authors.initials: N
authors.lastname: Tazato
authors.affiliation: Technical Department, TechnoSuruga Laboratory Co. Ltd, 330 Nagasaki, Shimizu-ku, Shizuoka-shi, Shizuoka 424-0065, Japan.
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Handa
authors.affiliation: Technical Department, TechnoSuruga Laboratory Co. Ltd, 330 Nagasaki, Shimizu-ku, Shizuoka-shi, Shizuoka 424-0065, Japan.
authors.forename: Junko
authors.initials: J
authors.lastname: Tomita
authors.affiliation: Independent Administrative Institution, National Research Institute for Cultural Properties, Tokyo, 13-43 Ueno-Koen, Taito-ku, Tokyo 110-8713, Japan.
authors.forename: Rika
authors.initials: R
authors.lastname: Kigawa
authors.affiliation: Independent Administrative Institution, National Research Institute for Cultural Properties, Tokyo, 13-43 Ueno-Koen, Taito-ku, Tokyo 110-8713, Japan.
authors.forename: Chie
authors.initials: C
authors.lastname: Sano
authors.affiliation: TechnoSuruga Laboratory Co., Ltd, Chiba Branch Office & Lab, No. 4 Sanko Building, Room 201, 3-1532-13 Hasama-cho, Funabashi-shi, Chiba 274-0822, Japan.
authors.forename: Junta
authors.initials: J
authors.lastname: Sugiyama
mesh_terms: D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D041821:Gluconacetobacter / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D007564:Japan; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D014451:Ubiquinone / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; C024989:coenzyme Q10