title: Recent advances in microfluidic cell separations.
abstract: The isolation and sorting of cells has become an increasingly important step in chemical and biological analyses. As a unit operation in more complex analyses, isolating a phenotypically pure cell population from a heterogeneous sample presents unique challenges. Microfluidic systems are ideal platforms for performing cell separations, enabling integration with other techniques and enhancing traditional separation modalities. In recent years there have been several techniques that use surface antigen affinity, physical interactions, or a combination of the two to achieve high separation purity and efficiency. This review discusses methods including magnetophoretic, acoustophoretic, sedimentation, electric, and hydrodynamic methods for physical separations. We also discuss affinity methods, including magnetic sorting, flow sorting, and affinity capture.
authors.affiliation: Department of Chemistry and Biochemistry, Texas Tech University, Lubbock, Texas 79409, USA.
authors.forename: Yan
authors.initials: Y
authors.lastname: Gao
authors.forename: Wenjie
authors.initials: W
authors.lastname: Li
authors.forename: Dimitri
authors.initials: D
authors.lastname: Pappas
mesh_terms: D000345:Affinity Labels / Q000378:metabolism; D000818:Animals; D002469:Cell Separation / Q000379:methods*; D006801:Humans; D046210:Microfluidic Analytical Techniques / Q000379:methods*; D055585:Physical Phenomena
chemical_list: D000345:Affinity Labels