title: Analysis of the SDS-PAGE patterns of outer membrane proteins from Escherichia coli strains that have lost the ability to form K1 antigen and varied in the susceptibility to normal human serum.
abstract: We used SDS-polyacrylamide gel electrophoresis to investigate the outer membrane proteins (OMPs) band composition of 19 Escherichia coli K1 strains that have spontaneously lost the ability to form K1 polysaccharide capsule (E. coli K1-) and demonstrated different degrees of susceptibility to the bactericidal action of normal human serum. Presented results showed that there were differences between E. coli K1- strains in OMPs expressing capacity. The analysis performed on OMPs has not revealed a direct association between the different OMPs band composition and the susceptibility of these strains to the serum.
authors.affiliation: Department of Biology and Medical Parasitology, Wroclaw Medical University, Mikulicza-Radeckiego 9, 50-367, Wrocław, Poland, agnieszka.cisowska@umed.wroc.pl.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Cisowska
authors.forename: Gabriela
authors.initials: G
authors.lastname: Bugla-Płoskońska
mesh_terms: D000942:Antigens, Bacterial; D016667:Bacterial Capsules / Q000235:genetics*; D001425:Bacterial Outer Membrane Proteins / Q000032:analysis*; D001770:Blood Bactericidal Activity; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000737:chemistry* / Q000302:isolation & purification / Q000502:physiology*; D004927:Escherichia coli Infections / Q000382:microbiology; D006801:Humans; D050296:Microbial Viability; D011135:Polysaccharides, Bacterial; D014552:Urinary Tract Infections / Q000382:microbiology
chemical_list: D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D011135:Polysaccharides, Bacterial; C036962:capsular polysaccharide K1