title: In vitro propagation of male germline stem cells from piglets.
abstract: OBJECTIVE
  To study the effects of serum and growth factors on propagation of porcine male germline stem cells (MGSCs) in vitro and develop a culture system for these stem cells.
  
  
  METHODS
  Fresh testicular cells from neonatal piglets were obtained by mechanical dissociation and collagenase-trypsin digestion. After differential plating, non-adhering cells were cultured in media supplemented with different concentrations of serum (0, 1 %, 2 %, 5 %, 10 %). After 10 days of primary culture, the cells were maintained in media supplemented with different concentrations of growth factors (basic fibroblast growth factor and epidermal growth factor at 1, 5, 10 ng/ml). The number of MGSC-derived colonies with different sizes was determined in each treatment to assess the effects of serum concentrations and growth factors.
  
  
  RESULTS
  The number of MGSC-derived colonies was significantly higher in the presence of 1 % rather than 10 % fetal bovine serum (FBS). Basic fibroblast growth factor (bFGF) at 1, 5 ng/ml and epidermal growth factor (EGF) at 5, 10 ng/ml significantly promoted colony formation. Immunocytochemistry, reverse transcriptase-polymerase chain reaction (RT-PCR) and xenotransplantation assays demonstrated the presence of functional stem cells in cultured cell population.
  
  
  CONCLUSIONS
  In vitro propagation of porcine MGSCs could be maintained in the presence of 1 % FBS and supplementation of growth factors for 1 month.
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, 22 Xi-nong Road, Yangling, Shaanxi, 712100, People's Republic of China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Zheng
authors.forename: Xiue
authors.initials: X
authors.lastname: Tian
authors.forename: Yaqing
authors.initials: Y
authors.lastname: Zhang
authors.forename: Jinzhou
authors.initials: J
authors.lastname: Qin
authors.forename: Junhui
authors.initials: J
authors.lastname: An
authors.forename: Wenxian
authors.initials: W
authors.lastname: Zeng
mesh_terms: D053687:Adult Stem Cells / Q000166:cytology* / Q000637:transplantation*; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D018929:Cell Culture Techniques; D049109:Cell Proliferation; D003470:Culture Media; D004815:Epidermal Growth Factor / Q000494:pharmacology; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology; D007248:Infertility, Male / Q000628:therapy; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D013091:Spermatogenesis; D013094:Spermatozoa / Q000378:metabolism; D013552:Swine; D013737:Testis / Q000166:cytology; D014183:Transplantation, Heterologous
chemical_list: D003470:Culture Media; D016222:Fibroblast Growth Factor 2; D004815:Epidermal Growth Factor; D000469:Alkaline Phosphatase