title: Direct effects of vardenafil on the ureter: in vitro investigation and potential clinical applications of intralumenal administration.
abstract: Local intralumenal administration of substances with beneficial effects over the ureter appears to be a very attractive route for the administration of drugs to the urinary system, avoiding systemic adverse reactions associated with per os administration. This work investigates whether vardenafil is a good candidate for local intralumenal ureteral administration. More precisely, we examined the effect of vardenafil on human ureter contractility and on the viability of cultured human urothelial cells (hUC) and bladder smooth muscle cells (hBSMC). Our study revealed that vardenafil induced a dose-depended relaxation of isolated human ureteral tissue. In addition, growth and cell viability of cultured hBSMC and hUC remained unaltered after vardenafil application. Based on the above, this selective phosphodiesterase type 5 inhibitor can be considered as a promising pharmacologic agent for local intralumenal administration in clinical conditions in which ureteral dilation might be beneficial.
authors.affiliation: 1 Department of Urology, University of Patras , Patras, Greece .
authors.forename: Evangelos
authors.initials: E
authors.lastname: Liatsikos
authors.forename: Iason
authors.initials: I
authors.lastname: Kyriazis
authors.forename: Jochen
authors.initials: J
authors.lastname: Neuhaus
authors.forename: Panagiotis
authors.initials: P
authors.lastname: Kallidonis
authors.forename: Ioannis
authors.initials: I
authors.lastname: Georgiopoulos
authors.forename: Toni
authors.initials: T
authors.lastname: Franz
authors.forename: Abdulrahman
authors.initials: A
authors.lastname: Al-Aown
authors.forename: George
authors.initials: G
authors.lastname: Sakellaropoulos
authors.forename: Jens-Uwe
authors.initials: JU
authors.lastname: Stolzenburg
mesh_terms: D002478:Cells, Cultured; D042783:Endothelial Cells / Q000187:drug effects; D006801:Humans; D007093:Imidazoles / Q000008:administration & dosage*; D009119:Muscle Contraction / Q000187:drug effects*; D009130:Muscle, Smooth / Q000187:drug effects / Q000473:pathology / Q000503:physiopathology; D032389:Myocytes, Smooth Muscle / Q000187:drug effects; D058986:Phosphodiesterase 5 Inhibitors / Q000008:administration & dosage; D010879:Piperazines / Q000008:administration & dosage*; D013450:Sulfones / Q000008:administration & dosage; D014227:Triazines / Q000008:administration & dosage; D014513:Ureter / Q000187:drug effects* / Q000473:pathology / Q000503:physiopathology; D014570:Urologic Diseases / Q000188:drug therapy* / Q000473:pathology / Q000503:physiopathology; D000069058:Vardenafil Dihydrochloride
chemical_list: D007093:Imidazoles; D058986:Phosphodiesterase 5 Inhibitors; D010879:Piperazines; D013450:Sulfones; D014227:Triazines; D000069058:Vardenafil Dihydrochloride