title: TRPM7 channels regulate proliferation and adipogenesis in 3T3-L1 preadipocytes.
abstract: Transient receptor potential melastatin-7 (TRPM7) channels are involved in many cellular physiological and pathological processes. The present study was designed to investigate the expression of TRPM7 channels and the potential role in regulating cell proliferation and adipogenesis in 3T3-L1 preadipocytes with approaches of whole-cell patch voltage-clamp, molecular biology, cell proliferation, adipogenesis, etc. We found that a TRPM7-like current was recorded with Mg(2+) -free pipette solution in 3T3-L1 preadipocytes, and the current was inhibited by intercellular free Mg(2+) . The TRPM7-like current was potentiated by acidic pH and inhibited by 2-aminoethoxydiphenyl borate (2-APB). RT-PCR, Western blot and immunocytochemistry revealed that gene and protein of TRPM7 channels were abundant in 3T3-L1 preadipocytes. Blockade of TRPM7 channels with 2-APB inhibited cell proliferation in 3T3-L1 cells. In addition, knockdown of TRPM7 with specific siRNA inhibited both proliferation and adipogenesis. The present study demonstrates for the first time that TRPM7 channels regulate cell cycle and adipogenesis of 3T3-L1 preadipocytes.
authors.affiliation: Department of Pharmacology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China; Li Ka Shing Faculty of Medicine, Department of Physiology and Medicine, University of Hong Kong, Hong Kong, China.
authors.forename: Kui-Hao
authors.initials: KH
authors.lastname: Chen
authors.forename: Xiao-Hui
authors.initials: XH
authors.lastname: Xu
authors.forename: Yi
authors.initials: Y
authors.lastname: Liu
authors.forename: Yan
authors.initials: Y
authors.lastname: Hu
authors.forename: Man-Wen
authors.initials: MW
authors.lastname: Jin
authors.forename: Gui-Rong
authors.initials: GR
authors.lastname: Li
mesh_terms: D041721:3T3-L1 Cells; D050156:Adipogenesis / Q000235:genetics*; D000818:Animals; D001896:Boron Compounds / Q000494:pharmacology; D049109:Cell Proliferation / Q000187:drug effects; D055785:Gene Knockdown Techniques; D051379:Mice; D018408:Patch-Clamp Techniques; D050053:TRPM Cation Channels / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000235:genetics*
chemical_list: D001896:Boron Compounds; D050053:TRPM Cation Channels; C109986:2-aminoethoxydiphenyl borate; C421903:Trpm7 protein, mouse