title: Distribution of dimethylarginine-dimethylaminohydrolase-II (DDAH2) gene polymorphism in hemodialysis patients.
abstract: OBJECTIVE
  to describe the distribution of single nucleotide polymorphisms (SNPs) at -449 (promoter region) DDAH 2 in hemodialysis (HD) patients.
  
  
  METHODS
  this study was a descriptive study, 56 HD patients and 30 healthy individuals were enrolled. Based on its etiology, the HD patient group was further divided into hypertension (HT) group and non-HT group. DNA was extracted from whole blood samples with a commercially available DNA isolation kit. Genotyping of the polymorphisms was performed using PCR-based SNP detection methods (Applied Biosystems, Carlsbad, USA) based on 5'-exonuclease activity assays for rs805305 (-449 G/C). Allelic variation was assessed in each participant.
  
  
  RESULTS
  heterozygotes were observed as the most abundant genotypes in both groups (70% in healthy individuals and 55% in HD patients), followed by GG genotype in the HD patients (30%), while CC (27%) was the second most common genotype polymorphism in the healthy individuals.
  
  
  CONCLUSIONS
  there is a significant difference in distribution of DDAH 2 gene polymorphism among HD patients compared to healthy individuals (p=0.01).
authors.affiliation: Department of Internal Medicine, Faculty of Medicine, Airlangga University, Surabaya, Indonesia. mochthaha@yahoo.com
authors.forename: Muhammad
authors.initials: M
authors.lastname: Thaha
authors.forename: Muhammad
authors.initials: M
authors.lastname: Yusuf
authors.forename: Maulana A
authors.initials: MA
authors.lastname: Empitu
authors.forename: Achmad
authors.initials: A
authors.lastname: Bakarman
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Tomino
mesh_terms: D000328:Adult; D000581:Amidohydrolases / Q000235:genetics*; D016022:Case-Control Studies; D016009:Chi-Square Distribution; D005260:Female; D005819:Genetic Markers; D005838:Genotype; D006801:Humans; D006973:Hypertension / Q000150:complications / Q000235:genetics; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D006435:Renal Dialysis; D051436:Renal Insufficiency, Chronic / Q000209:etiology / Q000235:genetics* / Q000628:therapy
chemical_list: D005819:Genetic Markers; D000581:Amidohydrolases; C059999:dimethylargininase