title: Characterization of transcriptional complexity during longissimus muscle development in bovines using high-throughput sequencing.
abstract: BACKGROUND
  Beef cattle are among the most economically important animals in the world because they are farmed for their meat and leather. However, a lack of genetic information remains an obstacle to understanding the mechanisms behind the development of this animal. Analysis of the beef cattle transcriptome and its expression profile data are essential to extending the genetic information resources for this species and would support studies on this animal.
  
  
  RESULTS
  RNA sequencing of beef cattle was performed using the Illumina High-Seq2000 platform. A total of 25,605,140 and 26,214,800 reads were sequenced for embryonic and adult pooled samples, respectively. We identified 24,464-29,994 novel transcript units in two pooled samples. In addition, 8,533-10,144 genes showed evidence of alternative splicing, in agreement with the finding that alternative 3' splicing is the most common type of alternative splicing event in cattle. We detected the expression levels of 16,174 genes, and 6,800 genes exhibited differential expression between the two pooled samples with a false discovery rate ≤0.001. Using GO enrichment and KEGG pathway analysis, multiple GO term and biological pathways were found to be significantly enriched for differentially expressed genes. In addition, we discovered that 30,618-31,334 putative single nucleotide polymorphisms were located in coding regions.
  
  
  CONCLUSIONS
  We obtained a high-quality beef cattle reference transcriptome using a high throughput sequencing approach, thereby providing a valuable resource for better understanding the beef cattle genome. The transcriptome data will facilitate future functional studies on the beef cattle genome and can be applied to breeding programs for cattle and closely related mammals.
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling, Shaanxi, People's Republic of China.
authors.forename: Hua
authors.initials: H
authors.lastname: He
authors.forename: Xiaolin
authors.initials: X
authors.lastname: Liu
mesh_terms: D017398:Alternative Splicing / Q000235:genetics; D000818:Animals; D002417:Cattle / Q000235:genetics* / Q000254:growth & development*; D020869:Gene Expression Profiling; D063990:Gene Ontology; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D024510:Muscle Development / Q000235:genetics*; D009132:Muscles / Q000378:metabolism*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014158:Transcription, Genetic; D059467:Transcriptome / Q000235:genetics