title: Mass production of the ginsenoside Rg3(S) through the combinative use of two glycoside hydrolases.
abstract: The ginsenoside Rg3(S), which is one of the exceptional components of Korean red ginseng extract, has been known to have anti-cancer, anti-metastatic, and anti-obesity effects. An enzymatic bioconversion method was developed to obtain the ginsenoside Rg3(S) with a high specificity, yield, and purity. Two glycoside hydrolases (BglBX10 and Abf22-3) were employed to produce Rg3(S) as a 100g unit. The conversion reaction transformed ginsenoside Rc to Rd using Abf22-3, followed by Rb1 and Rd to Rg3(S), using BglBX10. It was performed in a 10L jar fermenter at pH 6.0 and 37°C for 24h, with a high concentration of 50mg/ml of purified ginsenoside mixture obtained from ginseng roots. Finally, 144g of Rg3(S) was produced from 250g of root extract with 78±1.2% chromatographic purity. These results suggest that this enzymatic method would be useful in the preparation of ginsenoside Rg3(S) for the functional food and pharmaceutical industries.
authors.affiliation: KAIST Institute for Biocentury, Korea Advanced Institute of Science and Technology, 291 Daehak-ro, Yuseong-gu, Daejeon 305-701, Republic of Korea.
authors.forename: Jin-Kwang
authors.initials: JK
authors.lastname: Kim
authors.forename: Chang-Hao
authors.initials: CH
authors.lastname: Cui
authors.forename: Qingmei
authors.initials: Q
authors.lastname: Liu
authors.forename: Min-Ho
authors.initials: MH
authors.lastname: Yoon
authors.forename: Sun-Chang
authors.initials: SC
authors.lastname: Kim
authors.forename: Wan-Taek
authors.initials: WT
authors.lastname: Im
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D001711:Biotransformation; D005417:Flavobacterium / Q000201:enzymology* / Q000235:genetics; D036145:Ginsenosides / Q000737:chemistry* / Q000378:metabolism; D006026:Glycoside Hydrolases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D007934:Leuconostoc / Q000201:enzymology* / Q000235:genetics; D015394:Molecular Structure; D008970:Molecular Weight; D005894:Panax / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry* / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D036145:Ginsenosides; D010936:Plant Extracts; C097367:ginsenoside Rg3; D006026:Glycoside Hydrolases