title: In vitro assessment of the immunological significance of a human monoclonal antibody directed to the influenza a virus nucleoprotein.
abstract: Influenza A viruses cause annual epidemics and occasionally pandemics. Antibodies directed to the conserved viral nucleoprotein (NP) may play a role in immunity against various influenza A virus subtypes. Here, we assessed the immunological significance of a human monoclonal antibody directed to NP in vitro. This antibody bound to virus-infected cells but did not display virus-neutralizing activity, complement-dependent cell cytotoxicity, or opsonization of viral antigen for improved antigen presentation to CD8(+) T cells by dendritic cells.
authors.affiliation: Department of Viroscience, Erasmus MC, Rotterdam, the Netherlands.
authors.forename: Rogier
authors.initials: R
authors.lastname: Bodewes
authors.forename: Martina M
authors.initials: MM
authors.lastname: Geelhoed-Mieras
authors.forename: Jens
authors.initials: J
authors.lastname: Wrammert
authors.forename: Rafi
authors.initials: R
authors.lastname: Ahmed
authors.forename: Patrick C
authors.initials: PC
authors.lastname: Wilson
authors.forename: Ron A M
authors.initials: RA
authors.lastname: Fouchier
authors.forename: Albert D M E
authors.initials: AD
authors.lastname: Osterhaus
authors.forename: Guus F
authors.initials: GF
authors.lastname: Rimmelzwaan
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology*; D000914:Antibodies, Viral / Q000276:immunology*; D000956:Antigens, Viral / Q000276:immunology*; D006801:Humans; D009980:Influenza A virus / Q000276:immunology*; D019590:Nucleocapsid Proteins; D011485:Protein Binding; D016601:RNA-Binding Proteins / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology; D014758:Viral Core Proteins / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000956:Antigens, Viral; C048842:NP protein, Influenza A virus; D019590:Nucleocapsid Proteins; D016601:RNA-Binding Proteins; D014758:Viral Core Proteins