title: Effect of aqueous leaves extract of Ocimum gratissimum on hematological parameters in rats.
abstract: OBJECTIVE
  This study aims to elucidate the effect of Ocimum gratissimum on hematological parameters in rats.
  
  
  METHODS
  Thirty male albino Wistar rats were randomly assigned into three groups of ten rats each. Group 1 was control, while groups 2 (LD) and 3 (HD) received 500 mg/kg and 1000 mg/kg body weight, respectively, of the extract orally once daily. Rats in all three groups received normal rat chow and drinking water ad libitum for 28 days. Complete blood count was done using an automatic counter.
  
  
  RESULTS
  The HD group had significantly (P<.05) higher red blood cell (RBC) counts, packed cell volume (PCV), hemoglobin (Hb), and platelet counts as compared with the control and LD groups. No significant changes were observed in the total white blood cell (WBC) count of the three groups, but significantly (P<.05) lower lymphocyte and higher neutrophil counts were observed in the HD group compared with the LD group. The mean platelet volume (MPV), platelet-large cell ratio (P-LCR), and platelet distribution width (PDW) were significantly (P<.05) reduced in the HD compared with the LD group. The mean corpuscular volume (MCV) and RBC distribution width-standard deviation were significantly (P<.05) lower in the HD group than in control. No significant changes were observed in levels of mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), and RBC distribution width-coefficient of variation among the groups.
  
  
  CONCLUSIONS
  We conclude that oral administration of O gratissimum increases RBC, PCV, Hb, platelet count, and neutrophils and also leads to a decrease in platelet indices (i.e., MPV, P-LCR, and PDW).
authors.affiliation: Department of Physiology, College of Medical Sciences, University of Calabar, Calabar, Nigeria.
authors.forename: Oe
authors.initials: O
authors.lastname: Ofem
authors.forename: Ej
authors.initials: E
authors.lastname: Ani
authors.forename: Ae
authors.initials: A
authors.lastname: Eno
keywords: Blood indices; Hb; O gratissimum; differential white blood cell; packed cell volume; platelet; red blood cell; total WBC