title: 3M Petrifilm Environmental Listeria Plate.
abstract: A validation study of the 3M Petrifilm Environmental Listeria (EL) Plate (3M Food Safety, St. Paul, MN) was conducted at Q Laboratories, Inc., Cincinnati, OH. The method was compared to the Health Canada MFHPB-30 reference method for the analysis of stainless steel environmental surfaces. Twenty replicates of the environmental surface were analyzed at a low and a high inoculum level. The low-level sampling area was inoculated with 0.2-2 CFU/5 cm2, and the high-level sampling area was inoculated with 2-5 CFU/5 cm2. Five control replicates were also analyzed at 0 CFU/5 cm2. There was no significant difference in the number of positives detected by the 3M Petrifilm EL Plate method and the Health Canada MFHPB-30 reference method for the environmental surface analyzed in this study.
authors.affiliation: 3M Food Safety, 3M Center, Bldg 260-6B-01, Saint Paul, MN 55144-1000, USA. dbenesh1@mmm.com
authors.forename: Deann L
authors.initials: DL
authors.lastname: Benesh
authors.forename: Erin S
authors.initials: ES
authors.lastname: Crowley
authors.forename: Patrick M
authors.initials: PM
authors.lastname: Bird
mesh_terms: D001431:Bacteriological Techniques / Q000379:methods* / Q000592:standards*; D004783:Environmental Microbiology / Q000592:standards*; D008087:Listeria / Q000302:isolation & purification*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity