title: 4-Hydroxyhexenal- and 4-hydroxynonenal-modified proteins in pterygia.
abstract: Oxidative stress has been suspected of contributing to the pathogenesis of pterygia. We evaluated the immunohistochemical localization of the markers of oxidative stress, that is, the proteins modified by 4-hydroxyhexenal (4-HHE) and 4-hydroxynonenal (4-HNE), which are reactive aldehydes derived from nonenzymatic oxidation of n-3 and n-6 polyunsaturated fatty acids, respectively. In the pterygial head, labeling of 4-HHE- and 4-HNE-modified proteins was prominent in the nuclei and cytosol of the epithelium. In the pterygial body, strong labeling was observed in the nuclei and cytosol of the epithelium and proliferating subepithelial connective tissue. In normal conjunctival specimens, only trace immunoreactivity of both proteins was observed in the epithelial and stromal layers. Exposures of ultraviolet (330 nm, 48.32 ± 0.55 J/cm(2)) or blue light (400 nm, 293.0 ± 2.0 J/cm(2)) to rat eyes enhanced labeling of 4-HHE- and 4-HNE-modified proteins in the nuclei of conjunctival epithelium. Protein modifications by biologically active aldehydes are a molecular event involved in the development of pterygia.
authors.affiliation: Department of Ophthalmology, Shimane University Faculty of Medicine, Izumo, Shimane, Japan.
authors.forename: Ichiya
authors.initials: I
authors.lastname: Sano
authors.forename: Sachiko
authors.initials: S
authors.lastname: Kaidzu
authors.forename: Masaki
authors.initials: M
authors.lastname: Tanito
authors.forename: Katsunori
authors.initials: K
authors.lastname: Hara
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Okuno
authors.forename: Akihiro
authors.initials: A
authors.lastname: Ohira
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D000447:Aldehydes / Q000378:metabolism*; D000818:Animals; D003228:Conjunctiva / Q000378:metabolism / Q000473:pathology / Q000528:radiation effects; D005136:Eye Proteins / Q000378:metabolism*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D011625:Pterygium / Q000378:metabolism* / Q000473:pathology; D051381:Rats
chemical_list: D000447:Aldehydes; D005136:Eye Proteins; C063409:4-hydroxyhexenal; C027576:4-hydroxy-2-nonenal