title: Salt loading in canola oil fed SHRSP rats induces endothelial dysfunction.
abstract: This study aimed to determine if 50 days of canola oil intake in the absence or presence of salt loading affects: (1) antioxidant and oxidative stress markers, (2) aortic mRNA of NADPH oxidase (NOX) subunits and superoxide dismutase (SOD) isoforms and (3) endothelial function in SHRSP rats. SHRSP rats were fed a diet containing 10 wt/wt% soybean oil or 10 wt/wt% canola oil, and given tap water or water containing 1% NaCl for 50 days. Without salt, canola oil significantly increased RBC SOD, plasma cholesterol and triglycerides, aortic p22 (phox) , NOX2 and CuZn-SOD mRNA, and decreased RBC glutathione peroxidase activity. With salt, canola oil reduced RBC SOD and catalase activity, LDL-C, and p22 (phox) mRNA compared with canola oil alone, whereas plasma malondialdehyde (MDA) was reduced and RBC MDA and LDL-C were higher. With salt, the canola oil group had significantly reduced endothelium-dependent vasodilating responses to ACh and contractile responses to norepinephrine compared with the canola oil group without salt and to the WKY rats. These results indicate that ingestion of canola oil increases O2 (-) generation, and that canola oil ingestion in combination with salt leads to endothelial dysfunction in the SHRSP model.
authors.affiliation: School of Medicine, Deakin University, Victoria, Australia.
authors.forename: Annateresa
authors.initials: A
authors.lastname: Papazzo
authors.forename: Xavier A
authors.initials: XA
authors.lastname: Conlan
authors.forename: Louise
authors.initials: L
authors.lastname: Lexis
authors.forename: Fadi J
authors.initials: FJ
authors.lastname: Charchar
authors.forename: Paul A
authors.initials: PA
authors.lastname: Lewandowski
mesh_terms: D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D001011:Aorta / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D001794:Blood Pressure / Q000187:drug effects; D001835:Body Weight / Q000187:drug effects; D004327:Drinking Behavior / Q000187:drug effects; D004730:Endothelium, Vascular / Q000187:drug effects* / Q000503:physiopathology*; D005229:Fatty Acids, Monounsaturated / Q000494:pharmacology*; D005247:Feeding Behavior / Q000187:drug effects*; D005786:Gene Expression Regulation / Q000187:drug effects; D066298:In Vitro Techniques; D008055:Lipids / Q000097:blood; D009599:Nitroprusside / Q000494:pharmacology; D009638:Norepinephrine / Q000494:pharmacology; D018384:Oxidative Stress / Q000187:drug effects; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D000074262:Rapeseed Oil; D051381:Rats; D011918:Rats, Inbred SHR; D012965:Sodium Chloride / Q000494:pharmacology*; D013024:Soybean Oil / Q000494:pharmacology; D020521:Stroke / Q000473:pathology / Q000503:physiopathology*; D013599:Systole / Q000187:drug effects; D014867:Water
chemical_list: D000975:Antioxidants; D005229:Fatty Acids, Monounsaturated; D008055:Lipids; D012333:RNA, Messenger; D000074262:Rapeseed Oil; D014867:Water; D009599:Nitroprusside; D012965:Sodium Chloride; D013024:Soybean Oil; D000109:Acetylcholine; D009638:Norepinephrine