title: Analyses of associations between reactive oxygen metabolites and antioxidant capacity and related factors among healthy adolescents.
abstract: Evidence based on epidemiologic investigations using biochemical parameter is meaningful for health promotion and administration among adolescents. We conducted Reactive Oxygen Metabolites (ROM) and Biological Antioxidant Potentials (BAP) tests, along with a questionnaire survey, for a sample of 74 high school students (16.51±0.11 years of aged mean±SE), to investigate the associations between ROM, BAP, and related factors, including BMI and blood biochemical data. Venous blood samples (approximately 7cc) were collected. At the same time, each individual's information was obtained from the questionnaire. The mental health status was investigated using the Center for Epidemiologic Study Depression scale (CES-D) included in the same questionnaire. The mean values and standard errors of all variables were calculated. In addition, the relationships between ROM and BAP with these factors were analyzed. The results revealed the preferred levels of ROM (261.95 ± 9.52 U.CARR) and, BAP (2429.89±53.39 µmol/L) and blood biochemical data. Few significant relationships between two markers and related factors were found. So, we detected a cluster with an imbalance between ROM and BAP, which means low antioxidant ability, whereas the other clusters had conditions with moderate balance or good balance between them. Moreover, we determined the Oxidative stress-Antioxidant capacity ratio (OAR), using the ROM and BAP values, in order to clarify the characteristic of the detected clusters.However, comparative analyses across the three clusters did not yield significant differences in all related factors. No correlations between ROM, BAP and related factors were indicated, although significant association between ROM and BAP was observed (R2=0.1156, R=0.340, P=0.013). The reason for these results can be explained by the influences of good health and young age. On the other hand, present study suggests that some latent problems among adolescents may be related to unhealthy conditions in the future. Also, this study indicated that ROM and BAP may be useful as markers of the oxidative stress status. After this, further investigations using biomarkers based on epidemiologic design should be conducted, to reveal the reliability of the present results.
authors.forename: Kazuyoshi
authors.initials: K
authors.lastname: Tamae
authors.forename: Toshiharu
authors.initials: T
authors.lastname: Eto
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Aoki
authors.forename: Shingo
authors.initials: S
authors.lastname: Nakamaru
authors.forename: Kazunori
authors.initials: K
authors.lastname: Koshikawa
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Sakuma
authors.affiliation: Faculty of Education and Welfare Science, Oita University, Oita city, Oita, 870-1192, Japan. k-tamae@oita-u.ac.jp.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Hirano
mesh_terms: D000293:Adolescent; D000367:Age Factors; D000975:Antioxidants / Q000378:metabolism*; D015415:Biomarkers / Q000097:blood; D015992:Body Mass Index; D016000:Cluster Analysis; D005260:Female; D006304:Health Status; D064368:Healthy Volunteers; D006801:Humans; D008297:Male; D008603:Mental Health; D018384:Oxidative Stress; D017382:Reactive Oxygen Species / Q000097:blood*; D011795:Surveys and Questionnaires
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D017382:Reactive Oxygen Species