title: Characterization, tissue distribution and regulation of agouti-related protein (AgRP) in a cyprinid fish (Schizothorax prenanti).
abstract: Agouti-related protein (AgRP) is an important neuropeptide involved in the regulation of feeding in both mammals and fish. In this study, we have cloned the full-length cDNA sequence for AgRP in a cyprinid fish (Schizothorax prenanti). The AgRP gene, encoding 126-amino acids, was strongly expressed in the brain. The AgRP gene was detected in embryos at developmental stages. Further, its mRNA was detectable in unfertilized eggs. An experiment was conducted to determine the expression profile of AgRP during short-term and long-term fasting of the hypothalamus. The expression level of AgRP in unfed fish was significantly increased at 3 and 4h post-fasting than in fed fish but did not affect AgRP mRNA expression after 14 days fasting. Overall, our results suggest that AgRP is a conserved peptide that might be involved in the regulation of short-term feeding and other physiological function in Schizothorax prenanti.
authors.affiliation: Department of Aquaculture, Sichuan Agricultural University, 46# Xinkang Road, Yaan, China.
authors.forename: RongBin
authors.initials: R
authors.lastname: Wei
authors.forename: DengYue
authors.initials: D
authors.lastname: Yuan
authors.forename: Tao
authors.initials: T
authors.lastname: Wang
authors.forename: ChaoWei
authors.initials: C
authors.lastname: Zhou
authors.forename: FangJun
authors.initials: F
authors.lastname: Lin
authors.forename: Hu
authors.initials: H
authors.lastname: Chen
authors.forename: HongWei
authors.initials: H
authors.lastname: Wu
authors.forename: ShiYong
authors.initials: S
authors.lastname: Yang
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.forename: Ju
authors.initials: J
authors.lastname: Liu
authors.forename: YunDi
authors.initials: Y
authors.lastname: Gao
authors.forename: ZhiQiong
authors.initials: Z
authors.lastname: Li
mesh_terms: D054369:Agouti-Related Protein / Q000235:genetics / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000378:metabolism; D017124:Conserved Sequence; D003530:Cyprinidae / Q000235:genetics / Q000378:metabolism*; D005215:Fasting / Q000378:metabolism; D005260:Female; D029941:Fish Proteins / Q000235:genetics / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental; D007031:Hypothalamus / Q000378:metabolism*; D008297:Male; D008969:Molecular Sequence Data; D009928:Organ Specificity; D010802:Phylogeny; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
chemical_list: D054369:Agouti-Related Protein; D029941:Fish Proteins; D012333:RNA, Messenger
keywords: ARC; ASIP; AgRP; Appetite regulation; CART; Cloning; Expression pattern; LHRH; Luteinizing Hormone Releasing Hormone; MC3-R; MC4-R; MCRs; MS-222; NPY; ORF; Open reading frame; POMC; Schizothorax prenanti; Structure; TSGD; agouti-related protein; agouti-signaling protein; arcuate nucleus; cocaine and amphetamine regulated transcript; hour post fertilization; hpf; melanocortin receptor 3; melanocortin receptor 4; melanocortin receptors; neuropeptide Y; proopiomelanocortin; teleost-specific genome duplication; tricaine methanesulfonate