title: Rapid high-throughput assay to assess scavenging capacity index using DPPH.
abstract: A new microplate-adapted DPPH rapid assay was developed to assess the antioxidant capacity of pure compounds and foods. The assay was carried out in buffered medium (methanol: 10mmol/l Tris buffer pH 7.5, 1:1 v/v) and reaction was completed at 10min. The scavenging capacity index (SCI), a theoretical antioxidant parameter directly related to the antioxidant capacity of samples, was calculated. SCI for pure compounds: gallic acid (6.76±0.08), quercetin (7.89±0.24), catechin (6.05±0.23), trolox (2.32±0.03), ascorbic acid (2.52±0.15) and gluthatione (1.08±0.08) and foods (μmol DPPH scavenged/100ml): tropical juice (655.62±12.18), mediterraneo juice (702.87±11.13), apple juice (212.52±17.22), pomegranate juice (319.83±9.45), red grape nectar (1093.05±18.69), Don Simon orange juice (632.94±17.22) and date syrup (15992.34±250.7) were comparable to those in previous reports using the classic DPPH assay. The relative standard deviation (RSD) for the SCI on the same and different days was less than 8.12% in all cases.
authors.affiliation: Universidad Autónoma de Madrid, Facultad de Medicina, Departamento de Fisiología, Madrid, Spain.
authors.forename: Fatima
authors.initials: F
authors.lastname: Abderrahim
authors.forename: Silvia M
authors.initials: SM
authors.lastname: Arribas
authors.forename: M Carmen
authors.initials: MC
authors.lastname: Gonzalez
authors.forename: Luis
authors.initials: L
authors.lastname: Condezo-Hoyos
mesh_terms: D001628:Beverages / Q000032:analysis*; D001713:Biphenyl Compounds / Q000737:chemistry; D016166:Free Radical Scavengers / Q000737:chemistry*; D005638:Fruit / Q000737:chemistry*; D057166:High-Throughput Screening Assays / Q000295:instrumentation / Q000379:methods*; D027845:Malus / Q000737:chemistry*; D010851:Picrates / Q000737:chemistry; D028321:Plant Preparations / Q000737:chemistry*; D027843:Vitis / Q000737:chemistry*
chemical_list: D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D010851:Picrates; D028321:Plant Preparations; C004931:1,1-diphenyl-2-picrylhydrazyl