title: Effect of filgrastim (recombinant human granulocyte colony stimulating factor) on IgE responses in human asthma: a case study.
abstract: BACKGROUND
  The role of peripheral blood progenitor cell mobilization on Immunoglobulin E (IgE) responses has not been studied.
  
  
  METHODS
  Distributions of blood lymphocytes (CD4+, CD8+, CD8+CD60+, CD19+, CD23+, CD16/56+, CD25, CD45RA+, CD45RO+, CD34+), and levels of serum immunoglobulins (IgM, IgG, IgA, IgE) were studied in an allergic asthmatic serum IgE+ (181IU/mL) adult (m/45 y/o) donor undergoing routine stem cell mobilization protocol (American Society of Hematology) before (day-30), during (day 4), and after (1 wk post last dose) filgrastim (subcutaneous, 480 mcg, 2qd) treatment (flow cytometry, nephelometry, UniCAP Total IgE Fluoro enzyme immunoassay).
  
  
  RESULTS
  On day 4 of filgrastim treatment, numbers of CD8+CD60+T cells and CD23+ blood cells dramatically increased (98% and 240% respectively) compared with pre treatment. In contrast on day 4 of treatment, serum IgE levels decreased (>50%) compared with pre treatment. CD8+CD60+T cells and CD23+ blood cells and serum IgE levels approached pre-treatment levels at 1 week post treatment.
  
  
  CONCLUSIONS
  Filgrastim treatment transiently increases numbers of CD8+CD60+T and CD23+ expressing cells, which are known to regulate human IgE responses, while also transiently suppressing ongoing IgE responses. These results suggest that filgrastim affects IgE related responses, and may be useful in modulating allergic responses.
authors.affiliation: Departments of Pediatrics, Center for Allergy and Asthma Research at SUNY Downstate, State University of New York Downstate Medical Center, Brooklyn, NY 11203, United States. Electronic address: tamar.smith-norowitz@downstate.edu.
authors.forename: Tamar A
authors.initials: TA
authors.lastname: Smith-Norowitz
authors.forename: Rauno
authors.initials: R
authors.lastname: Joks
authors.forename: Kevin B
authors.initials: KB
authors.lastname: Norowitz
authors.forename: Seto
authors.initials: S
authors.lastname: Chice
authors.forename: Helen G
authors.initials: HG
authors.lastname: Durkin
authors.forename: Martin H
authors.initials: MH
authors.lastname: Bluth
mesh_terms: D000328:Adult; D015703:Antigens, CD / Q000097:blood; D001249:Asthma / Q000097:blood*; D000069585:Filgrastim; D016179:Granulocyte Colony-Stimulating Factor / Q000008:administration & dosage*; D019650:Hematopoietic Stem Cell Mobilization; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood*; D008214:Lymphocytes / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D011994:Recombinant Proteins / Q000008:administration & dosage
chemical_list: D015703:Antigens, CD; D011994:Recombinant Proteins; D016179:Granulocyte Colony-Stimulating Factor; D007073:Immunoglobulin E; D000069585:Filgrastim
keywords: Ab; Asthma; CD8+CD60+T cells; EDTA; Filgrastim; Ig; IgE; PBSC; T helper; Th; WBC; antibody; ethylenediaminotetraacetic; immunoglobulin; mAbs; monoclonal antibodies; peripheral blood stem cells; recombinant human granulocyte colony-stimulating factor; rhG-CSF; white blood cell