title: High-throughput sequencing of methylated cytosine enriched by modification-dependent restriction endonuclease MspJI.
abstract: BACKGROUND
  As a well-known epigenomic modification, DNA methylation is found to be common in plants and plays an important role in many biological processes. Relying on the unique feature of methylation-dependent digestion, the family of methylation-requiring restriction-like endonuclease, such as MspJI and its homologs, was suggested for a potential usage in methylation detection.
  
  
  RESULTS
  In this study, we combine MspJI digestion and electrophoretic band selection with next generation high-throughput sequencing technology to detect 5-methylcytosines in Arabidopsis genome. By developing a bioinformatics workflow to attribute the CNNR sites recognized by MspJI to the reference genome, we fulfilled the systematic assessment of this method.
  
  
  CONCLUSIONS
  According to the assessment, here we provide the method for generating a detailed map of plant methylome that could be feasible, reliable and economical in methylation investigation.
authors.affiliation: Science & Technology Department, BGI-Shenzhen, Building No, 11, Bei Shan Industrial Zone, Yantian District, Shenzhen 518083, China.
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Huang
authors.forename: Hanlin
authors.initials: H
authors.lastname: Lu
authors.forename: Jun-Wen
authors.initials: JW
authors.lastname: Wang
authors.forename: Liqin
authors.initials: L
authors.lastname: Xu
authors.forename: Siyang
authors.initials: S
authors.lastname: Liu
authors.forename: Jihua
authors.initials: J
authors.lastname: Sun
authors.forename: Fei
authors.initials: F
authors.lastname: Gao
mesh_terms: D017360:Arabidopsis / Q000235:genetics; D003596:Cytosine / Q000378:metabolism*; D019175:DNA Methylation; D004262:DNA Restriction Enzymes / Q000378:metabolism*; D059014:High-Throughput Nucleotide Sequencing
chemical_list: D003596:Cytosine; D004262:DNA Restriction Enzymes