title: Bacillus cereus in infant foods: prevalence study and distribution of enterotoxigenic virulence factors in Isfahan Province, Iran.
abstract: This study was carried out in order to investigate the presences of Bacillus cereus and its enterotoxigenic genes in infant foods in Isfahan, Iran. Overall 200 infant foods with various based were collected and immediately transferred to the laboratory. All samples were culture and the genomic DNA was extracted from colonies with typical characters of Bacillus cereus. The presences of enterotoxigenic genes were investigated using the PCR technique. Eighty-four of two hundred samples (42%) were found to be contaminated with B. cereus with a ranges of 3 × 10(1)-9.3 × 10(1) spore per gram sample. Totally, entFM had the highest (61.90%) incidences of enterotoxigenic genes while hblA had the lowest (13.09%) incidences of enterotoxigenic genes. Overall, 6.7% of B. cereus isolates had all studied enetrotoxigenic genes while 25.5% of B. cereus strains had all studied enetrotoxigenic genes expectance bceT gene. This study is the first prevalence report of B. cereus and its enterotoxigenic genes in infant foods in Iran. Results showed that the infant food is one of the main sources of enterotoxigenic genes of B. cereus in Iran. Therefore, the accurate food inspection causes to reducing outbreak of diseases.
authors.affiliation: Department of Food Hygiene and Public Health, College of Veterinary Medicine, Islamic Azad University, Shahrekord Branch, P.O. Box 166, Shahrekord, Iran. ebrahimrahimi55@yahoo.com
authors.forename: Ebrahim
authors.initials: E
authors.lastname: Rahimi
authors.forename: Fahimeh
authors.initials: F
authors.lastname: Abdos
authors.forename: Hassan
authors.initials: H
authors.lastname: Momtaz
authors.forename: Zienab Torki
authors.initials: ZT
authors.lastname: Baghbadorani
authors.forename: Mohammad
authors.initials: M
authors.lastname: Jalali
mesh_terms: D001409:Bacillus cereus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D001427:Bacterial Toxins / Q000032:analysis*; D005504:Food Analysis / Q000379:methods*; D005506:Food Contamination / Q000032:analysis*; D005516:Food Microbiology / Q000379:methods*; D007225:Infant Food / Q000382:microbiology*; D007492:Iran
chemical_list: D001427:Bacterial Toxins