title: Viral categorization and discovery in human circulation by transcriptome sequencing.
abstract: Serum is the most common and easily accessible patient specimen in a minimally invasive manner. As a biological resource, RNA in serum has been less explored for its clinical utilization due to prevailing concerns regarding its high degradable nature. In the current study, however, we have documented the use of human serum RNA for viral categorization and discovery through transcriptome sequencing and analysis using well-curated databases and advanced bioinformatic tools. Such an integrated approach may have an immediate application in any clinical situations concerning with viral etiology.
authors.affiliation: Division of Gastroenterology and Hepatology, Department of Internal Medicine, Saint Louis University School of Medicine, St. Louis, MO 63104, USA.
authors.forename: Weihua
authors.initials: W
authors.lastname: Wang
authors.forename: Xiaoan
authors.initials: X
authors.lastname: Zhang
authors.forename: Yanjuan
authors.initials: Y
authors.lastname: Xu
authors.forename: Adrian M
authors.initials: AM
authors.lastname: Di Bisceglie
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Fan
mesh_terms: D019295:Computational Biology; D018076:DNA, Complementary / Q000235:genetics; D020869:Gene Expression Profiling / Q000379:methods*; D016174:Hepacivirus / Q000235:genetics / Q000302:isolation & purification*; D019698:Hepatitis C, Chronic / Q000175:diagnosis; D006801:Humans; D016366:Open Reading Frames; D012367:RNA, Viral / Q000097:blood* / Q000235:genetics; D012680:Sensitivity and Specificity; D059467:Transcriptome
chemical_list: D018076:DNA, Complementary; D012367:RNA, Viral
keywords: Multiple displacement amplification; Next-generation sequencing; Viral discovery