title: Polymer gene delivery vectors encapsulated in thermally sensitive bioreducible shell.
abstract: Stable, nanosized polyelectrolyte complexes between rationally designed thermally sensitive block copolymers and plasmid DNA (polyplexes) were formed and their in vitro transfection efficiency was tested. The polyplexes were further stabilized through encapsulation into a biodegradable polymer shell. Although reduced as compared to that of the corresponding polyplexes, the encapsulated systems still show acceptable transfection efficiency. That opens the possibility to tune the balance between the safe transport and efficient delivery of DNA into the cells.
authors.affiliation: Institute of Polymers, Bulgarian Academy of Sciences, Sofia 1113, Bulgaria.
authors.forename: Emilya D
authors.initials: ED
authors.lastname: Ivanova
authors.forename: Nadya I
authors.initials: NI
authors.lastname: Ivanova
authors.forename: Margarita D
authors.initials: MD
authors.lastname: Apostolova
authors.forename: Sevdalina C
authors.initials: SC
authors.lastname: Turmanova
authors.forename: Ivaylo V
authors.initials: IV
authors.lastname: Dimitrov
mesh_terms: D000178:Acrylamides / Q000737:chemistry; D000180:Acrylic Resins; D005822:Genetic Vectors / Q000378:metabolism*; D057809:HEK293 Cells; D006801:Humans; D053758:Nanoparticles / Q000737:chemistry; D010316:Particle Size; D011107:Polylysine / Q000737:chemistry; D011108:Polymers / Q000737:chemistry*; D014162:Transfection
chemical_list: D000178:Acrylamides; D000180:Acrylic Resins; D011108:Polymers; D011107:Polylysine; C052970:poly-N-isopropylacrylamide