title: Detection of mRNA in living cells by double-stranded locked nucleic acid probes.
abstract: Double-stranded probes are homogeneous biosensors for rapid detection of specific nucleotide sequences. These double-stranded probes have been applied in various molecular sensing applications, such as real-time polymerase chain reaction and detection of bacterial 16S rRNA. In this study, we present the design and optimization of double-stranded probes for single-cell gene expression analysis in living cells. With alternating DNA/LNA monomers for optimizing the stability and specificity, we show that the probe is stable in living cells for over 72 hours post-transfection and is capable of detecting changes in gene expression induced by external stimuli. The probes can be delivered to a large number of cells simultaneously by cationic liposomal transfection or to individual cells selectively by photothermal delivery. We also demonstrate that the probe quantifies intracellular mRNA in living cells through the use of an equilibrium analysis. With its effectiveness and performance, the double-stranded probe represents a broadly applicable approach for large-scale single-cell gene expression analysis toward numerous biomedical applications, such as systems biology, cancer, and drug screening.
authors.affiliation: Department of Aerospace and Mechanical Engineering, The University of Arizona, Tucson, AZ 85721-0119, USA.
authors.forename: Reza
authors.initials: R
authors.lastname: Riahi
authors.forename: Zachary
authors.initials: Z
authors.lastname: Dean
authors.forename: Ting-Hsiang
authors.initials: TH
authors.lastname: Wu
authors.forename: Michael A
authors.initials: MA
authors.lastname: Teitell
authors.forename: Pei-Yu
authors.initials: PY
authors.lastname: Chiou
authors.forename: Donna D
authors.initials: DD
authors.lastname: Zhang
authors.forename: Pak Kin
authors.initials: PK
authors.lastname: Wong
mesh_terms: D000199:Actins / Q000235:genetics; D045744:Cell Line, Tumor; D002470:Cell Survival; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics; D051547:Heme Oxygenase-1 / Q000235:genetics; D006801:Humans; D042541:Intracellular Space / Q000378:metabolism; D015345:Oligonucleotide Probes / Q000737:chemistry* / Q000378:metabolism*; D009841:Oligonucleotides / Q000737:chemistry* / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism
chemical_list: D000199:Actins; D018840:HSP70 Heat-Shock Proteins; D015345:Oligonucleotide Probes; D009841:Oligonucleotides; D012333:RNA, Messenger; C477371:locked nucleic acid; D051547:Heme Oxygenase-1