title: Determination of ethyl carbamate in wine by high performance liquid chromatography.
abstract: Kinetics of pre-column derivatization with 9-xanthydrol for the determination of ethyl carbamate (EC) in wine by a previous high performance liquid chromatographic method with fluorescence detection was studied and further developed. The life-time of the derivatized product and its excitation/absorption spectra were systematically investigated. Using low acidity (pH=2.5 set by phosphate buffers) only 3% of 9-xanthyl ethyl carbamate (XEC) decomposes in ∼48h, allowing a prolonged storage time of the derivatized EC conferring more accurate determination for large sample batches. Detection limit of this method is 3μgL(-1), while its average recovery is 98.5±4.9%. Calibration is linear up to 400μgL(-1). The EC content in 33 Hungarian wine samples ranges from 4.9 to 39.9μgL(-1) (average: 17.7μgL(-1), median: 16.7μgL(-1)), while only three of them was slightly over 30μgL(-1) EC, it being the maximum allowed concentration in countries already having legislation.
authors.affiliation: Department of Food Quality Assurance, Institute of Food Sciences, Faculty of Agricultural and Food Sciences, University of West Hungary, Lucsony u. 15-17, H-9200 Mosonmagyaróvár, Hungary.
authors.forename: Zsolt
authors.initials: Z
authors.lastname: Ajtony
authors.forename: Norbert
authors.initials: N
authors.lastname: Szoboszlai
authors.forename: László
authors.initials: L
authors.lastname: Bencs
authors.forename: Erna
authors.initials: E
authors.lastname: Viszket
authors.forename: Victor G
authors.initials: VG
authors.lastname: Mihucz
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D057230:Limit of Detection; D014520:Urethane / Q000032:analysis*; D014920:Wine / Q000032:analysis*
chemical_list: D014520:Urethane