title: Study on osteogenesis promoted by low sound pressure level infrasound in vivo and some underlying mechanisms.
abstract: To clarify the effects of low sound pressure level (LSPL) infrasound on local bone turnover and explore its underlying mechanisms, femoral defected rats were stabilized with a single-side external fixator. After exposure to LSPL infrasound for 30min twice everyday for 6 weeks, the pertinent features of bone healing were assessed by radiography, peripheral quantitative computerized tomography (pQCT), histology and immunofluorescence assay. Infrasound group showed a more consecutive and smoother process of fracture healing and modeling in radiographs and histomorphology. It also showed significantly higher average bone mineral content (BMC) and bone mineral density (BMD). Immunofluorescence showed increased expression of calcitonin gene related peptide (CGRP) and decreased Neuropeptide Y (NPY) innervation in microenvironment. The results suggested the osteogenesis promotion effects of LSPL infrasound in vivo. Neuro-osteogenic network in local microenvironment was probably one target mediating infrasonic osteogenesis, which might provide new strategy to accelerate bone healing and remodeling.
authors.affiliation: Department of Orthopaedics, Tangdu Hospital, Fourth Military Medical University, Xi'an, Shaanxi 710038, PR China. Electronic address: longhuadr@gmail.com.
authors.forename: Hua
authors.initials: H
authors.lastname: Long
authors.affiliation: Department of Orthotraumatology, Centro Hospitalar Conde de Sao Januario, Macau. Electronic address: cheanglekhang@yahoo.com.hk.
authors.forename: Liheng
authors.initials: L
authors.lastname: Zheng
authors.affiliation: Department of Rehabilitation, Centro Hospitalar Conde de Sao Januario, Macau. Electronic address: drfcko@yahoo.com.
authors.forename: Fernando Cardoso
authors.initials: FC
authors.lastname: Gomes
authors.affiliation: Department of Orthotraumatology, Centro Hospitalar Conde de Sao Januario, Macau. Electronic address: petercheungmc@yahoo.com.hk.
authors.forename: Jinhui
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Physiotherapy and Rehabilitation, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi 710032, PR China. Electronic address: pro.mu@fmmu.edu.cn.
authors.forename: Xiang
authors.initials: X
authors.lastname: Mou
authors.affiliation: Department of Physiotherapy and Rehabilitation, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi 710032, PR China. Electronic address: yuanhua@fmmu.edu.cn.
authors.forename: Hua
authors.initials: H
authors.lastname: Yuan
mesh_terms: D000162:Acoustics; D000818:Animals; D015519:Bone Density; D016723:Bone Remodeling; D015740:Calcitonin Gene-Related Peptide / Q000378:metabolism; D004195:Disease Models, Animal; D016267:External Fixators; D005264:Femoral Fractures / Q000000981:diagnostic imaging / Q000378:metabolism / Q000503:physiopathology / Q000628:therapy*; D005269:Femur / Q000000981:diagnostic imaging / Q000293:injuries / Q000294:innervation / Q000378:metabolism / Q000503:physiopathology* / Q000601:surgery; D005455:Fluorescent Antibody Technique; D017102:Fracture Healing; D008297:Male; D009478:Neuropeptide Y / Q000378:metabolism; D010012:Osteogenesis; D010027:Osteotomy / Q000295:instrumentation; D011312:Pressure; D051381:Rats; D017207:Rats, Sprague-Dawley; D013016:Sound; D013997:Time Factors; D014057:Tomography, X-Ray Computed
chemical_list: D009478:Neuropeptide Y; D015740:Calcitonin Gene-Related Peptide
keywords: CGRP; Infrasound; Innervation; NPY; Neuro-osteogenic network; Osteogenesis