title: Comparative Transcriptional Profiling of Two Contrasting Barley Genotypes under Salinity Stress during the Seedling Stage.
abstract: Salinity is one of the major abiotic stresses that affect crop productivity. Identification of the potential novel genes responsible for salt tolerance in barley will contribute to understanding the molecular mechanism of barley responses to salt stress. We compared changes in transcriptome between Hua 11 (a salt-tolerant genotype) and Hua 30 (a salt sensitive genotype) in response to salt stress at the seedling stage using barley cDNA microarrays. In total, 557 and 247 salt-responsive genes were expressed exclusively in the shoot and root tissue of the salt-tolerant genotype, respectively. Among these genes, a number of signal-related genes, transcription factors and compatible solutes were identified and some of these genes were carefully discussed. Notably, a LysM RLK was firstly found involved in salt stress response. Moreover, key enzymes in the pathways of jasmonic acid biosynthesis, lipid metabolism and indole-3-acetic acid homeostasis were specifically affected by salt stress in salt tolerance genotype. These salt-responsive genes and biochemical pathways identified in this study could provide further information for understanding the mechanisms of salt tolerance in barley.
authors.affiliation: Biotech Research Institute, Shanghai Academy of Agricultural Sciences, Beidi Road 2901, Minhang District, Shanghai 201106, China ; Shanghai Key Laboratory of Agricultural Genetics and Breeding, Shanghai 201106, China.
authors.forename: Runhong
authors.initials: R
authors.lastname: Gao
authors.forename: Ke
authors.initials: K
authors.lastname: Duan
authors.forename: Guimei
authors.initials: G
authors.lastname: Guo
authors.forename: Zhizhao
authors.initials: Z
authors.lastname: Du
authors.forename: Zhiwei
authors.initials: Z
authors.lastname: Chen
authors.forename: Liang
authors.initials: L
authors.lastname: Li
authors.forename: Ting
authors.initials: T
authors.lastname: He
authors.forename: Ruiju
authors.initials: R
authors.lastname: Lu
authors.forename: Jianhua
authors.initials: J
authors.lastname: Huang