title: Pistachio (Pistacia vera L.) is a new natural host of Hop stunt viroid.
abstract: Besides hop, Hop stunt viroid (HpSVd) infects many woody species including grapevine, citrus, peach, plum, apricot, almond, pomegranate, mulberry and jujube. Here, we report the first detection of HpSVd in pistachio (Pistacia vera L.). Samples corresponding to 16 pistachio cultivars were obtained from a nearby almond collection. From these samples, low molecular weight RNAs were extracted for double polyacrylamide gel electrophoresis, northern-blot analysis and reverse transcription polymerase chain reaction assays. HpSVd was detected in 4 of the 16 pistachio cultivars in the first year and in 6 in the second, being also detected in the almond collection. Examination of the nucleotide sequences of pistachio and almond isolates revealed 13 new sequence variants. Sequences from pistachio shared 92-96 % similarity with the first reported HpSVd sequence (GenBank X00009), and multiple alignment and phylogenetic analyses showed that one pistachio isolate (HpSVdPis67Jabari) clustered with the plum group, whereas all the others clustered with the hop, and the recombinants plum-citrus and plum-Hop/cit3 groups. By identifying pistachio as a new natural host, we confirm that HpSVd is an ubiquitous and genetically variable viroid that infects many different fruit trees cultivated worldwide.
authors.affiliation: Laboratoire des Biotechnologies Végétales Appliquées à l'Amélioration des Cultures, Faculté des Sciences de Sfax, Université de Sfax, BP 1171, 3000, Sfax, Tunisia, amineelleuch@hotmail.com.
authors.forename: Amine
authors.initials: A
authors.lastname: Elleuch
authors.forename: Imen
authors.initials: I
authors.lastname: Hamdi
authors.forename: Olfa
authors.initials: O
authors.lastname: Ellouze
authors.forename: Mohamed
authors.initials: M
authors.lastname: Ghrab
authors.forename: Hatem
authors.initials: H
authors.lastname: Fkahfakh
authors.forename: Noureddine
authors.initials: N
authors.lastname: Drira
mesh_terms: D015152:Blotting, Northern; D016000:Cluster Analysis; D004591:Electrophoresis, Polyacrylamide Gel; D008969:Molecular Sequence Data; D010802:Phylogeny; D027927:Pistacia / Q000821:virology*; D010935:Plant Diseases / Q000821:virology*; D012367:RNA, Viral / Q000235:genetics / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D014772:Viroids / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*
chemical_list: D012367:RNA, Viral