title: Oral administration of live exopolysaccharide-producing Pediococcus parvulus, but not purified exopolysaccharide, suppressed Enterobacteriaceae without affecting bacterial diversity in ceca of mice.
abstract: Growing evidence indicates that the gut microbiota could have an important role in the development of diet- and lifestyle-induced diseases. It has been shown that modulation of the gut microbiota by means of probiotics and prebiotics could improve host health. An oat-based product fermented by the exopolysaccharide (EPS)-producing organism Pediococcus parvulus 2.6 has been reported to have a bifidogenic effect. To find out whether the effect could be attributed to the EPS or the bacterium, mice were fed a diet supplemented with 2% purified EPS or 10(8) CFU/g of live P. parvulus 2.6 for 6 weeks. Both supplementations altered the gut microbiota composition but in different directions. Purified EPS not only significantly lowered the microbial diversity (P < 0.001) but decreased the bifidobacterial population (P = 0.01). In contrast, the live EPS-producing bacterium P. parvulus 2.6 antagonized Enterobacteriaceae without disturbing the homeostasis of the cecal microbiota.
authors.affiliation: Division of Biotechnology, Department of Chemistry, Lund University, Lund, Sweden. cecilia.lindstrom@biotek.lu.se
authors.forename: Cecilia
authors.initials: C
authors.lastname: Lindström
authors.forename: Jie
authors.initials: J
authors.lastname: Xu
authors.forename: Rickard
authors.initials: R
authors.lastname: Oste
authors.forename: Olle
authors.initials: O
authors.lastname: Holst
authors.forename: Göran
authors.initials: G
authors.lastname: Molin
mesh_terms: D000821:Animal Feed / Q000032:analysis; D000818:Animals; D018554:Avena / Q000378:metabolism; D001419:Bacteria / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D018407:Bacterial Physiological Phenomena; D001835:Body Weight / Q000187:drug effects; D002432:Cecum / Q000187:drug effects* / Q000382:microbiology*; D019587:Dietary Supplements / Q000032:analysis; D004755:Enterobacteriaceae / Q000187:drug effects / Q000502:physiology; D005247:Feeding Behavior / Q000187:drug effects; D005260:Female; D005285:Fermentation; D054892:Metagenome / Q000187:drug effects; D051379:Mice; D010376:Pediococcus / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D011134:Polysaccharides / Q000008:administration & dosage*; D019936:Probiotics / Q000008:administration & dosage*; D012336:RNA, Ribosomal, 16S / Q000235:genetics / Q000378:metabolism
chemical_list: D011134:Polysaccharides; D012336:RNA, Ribosomal, 16S