title: Global profiling in vestibular schwannomas shows critical deregulation of microRNAs and upregulation in those included in chromosomal region 14q32.
abstract: BACKGROUND
  Vestibular schwannomas are benign tumors that arise from Schwann cells in the VIII cranial pair and usually present NF2 gene mutations and/or loss of heterozygosity on chromosome 22q. Deregulation has also been found in several genes, such as ERBB2 and NRG1. MicroRNAs are non-coding RNAs approximately 21 to 23 nucleotides in length that regulate mRNAs, usually by degradation at the post-transcriptional level.
  
  
  METHODS
  We used microarray technology to test the deregulation of miRNAs and other non-coding RNAs present in GeneChip miRNA 1.0 (Affymetrix) over 16 vestibular schwannomas and 3 control-nerves, validating 10 of them by qRT-PCR.
  
  
  RESULTS
  Our results showed the deregulation of 174 miRNAs, including miR-10b, miR-206, miR-183 and miR-204, and the upregulation of miR-431, miR-221, miR-21 and miR-720, among others. The results also showed an aberrant expression of other non-coding RNAs. We also found a general upregulation of the miRNA cluster located at chromosome 14q32.
  
  
  CONCLUSIONS
  Our results suggest that several miRNAs are involved in tumor formation and/or maintenance and that global upregulation of the 14q32 chromosomal site contains miRNAs that may represent a therapeutic target for this neoplasm.
authors.affiliation: Neuro-Oncology Laboratory, Research Unit, La Paz University Hospital, IdiPAZ, Madrid, Spain. migtorres.martin@gmail.com
authors.forename: Miguel
authors.initials: M
authors.lastname: Torres-Martin
authors.forename: Luis
authors.initials: L
authors.lastname: Lassaletta
authors.forename: Jose M
authors.initials: JM
authors.lastname: de Campos
authors.forename: Alberto
authors.initials: A
authors.lastname: Isla
authors.forename: Javier
authors.initials: J
authors.lastname: Gavilan
authors.forename: Giovanny R
authors.initials: GR
authors.lastname: Pinto
authors.forename: Rommel R
authors.initials: RR
authors.lastname: Burbano
authors.forename: Farida
authors.initials: F
authors.lastname: Latif
authors.forename: Barbara
authors.initials: B
authors.lastname: Melendez
authors.forename: Javier S
authors.initials: JS
authors.lastname: Castresana
authors.forename: Juan A
authors.initials: JA
authors.lastname: Rey
mesh_terms: D000328:Adult; D000483:Alleles; D002883:Chromosomes, Human, Pair 14 / Q000235:genetics*; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods*; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D009464:Neuroma, Acoustic / Q000235:genetics*; D022661:RNA, Untranslated / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D035683:MicroRNAs; D022661:RNA, Untranslated