title: Control of human vascular tone by prostanoids derived from perivascular adipose tissue.
abstract: Perivascular adipose tissue (PVAT) surrounds most vessels and has now been recognized as a regulator of vascular functions. This effect of PVAT has been mostly demonstrated in vessels obtained from rats and mice. Thus, the aim of this study was to investigate anti-contractile effect of PVAT surrounding human coronary bypass grafts such as saphenous vein (SV) and internal mammary artery (IMA). Moreover, we aimed to determine the involvement of prostanoids in the anticontractile effect of PVAT. Human SV and IMA preparations were set up in an organ bath. The presence of PVAT in SV and IMA preparations significantly attenuated the contractile response to noradrenaline (NA). Preincubation with indomethacin, a cyclooxygenase inhibitor, increased NA contraction in SV preparations with PVAT. This effect was not observed in IMA preparation with PVAT incubated with indomethacin. The lower measurements of prostaglandin E2 (PGE2) released from PVAT surrounding IMA versus SV supported these effects. In conclusion, our results show that PVAT of SV could attenuate NA-induced contraction by releasing both PGE2 and prostacyclin (PGI2). In contrast to SV, PVAT of IMA exerts its anti-contractile effect independently from prostanoids. These observations suggest that retaining PVAT in human SV and IMA preparations may have potential clinical implications to improve coronary bypass graft patency.
authors.affiliation: Istanbul University, Faculty of Pharmacy, Department of Pharmacology, Istanbul, Turkey.
authors.forename: Gulsev
authors.initials: G
authors.lastname: Ozen
authors.forename: Gokce
authors.initials: G
authors.lastname: Topal
authors.forename: Ingrid
authors.initials: I
authors.lastname: Gomez
authors.forename: Arézou
authors.initials: A
authors.lastname: Ghorreshi
authors.forename: Kamel
authors.initials: K
authors.lastname: Boukais
authors.forename: Chabha
authors.initials: C
authors.lastname: Benyahia
authors.forename: Larry
authors.initials: L
authors.lastname: Kanyinda
authors.forename: Dan
authors.initials: D
authors.lastname: Longrois
authors.forename: Onder
authors.initials: O
authors.lastname: Teskin
authors.forename: B Sonmez
authors.initials: BS
authors.lastname: Uydes-Dogan
authors.forename: Xavier
authors.initials: X
authors.lastname: Norel
mesh_terms: D052436:Adipose Tissue, White / Q000378:metabolism*; D000368:Aged; D015232:Dinoprostone / Q000494:pharmacology / Q000502:physiology*; D011464:Epoprostenol / Q000494:pharmacology / Q000502:physiology*; D005260:Female; D006801:Humans; D007213:Indomethacin / Q000494:pharmacology; D020128:Inhibitory Concentration 50; D008297:Male; D008323:Mammary Arteries / Q000187:drug effects / Q000502:physiology*; D008875:Middle Aged; D009119:Muscle Contraction; D009131:Muscle, Smooth, Vascular / Q000187:drug effects / Q000502:physiology; D009638:Norepinephrine / Q000494:pharmacology; D012501:Saphenous Vein / Q000187:drug effects / Q000502:physiology*; D046509:Tissue Culture Techniques; D014661:Vasoconstriction; D014662:Vasoconstrictor Agents / Q000494:pharmacology
chemical_list: D014662:Vasoconstrictor Agents; D011464:Epoprostenol; D015232:Dinoprostone; D009638:Norepinephrine; D007213:Indomethacin
keywords: Human; Internal mammary artery; Perivascular adipose tissue; Prostanoids; Saphenous vein