title: Determination of urine cofilin-1 level in acute kidney injury using a high-throughput localized surface plasmon-coupled fluorescence biosensor.
abstract: The actin-depolymerizing factor (ADF)/cofilin protein family has been reported to be associated with ischemia-induced renal disorders. We examine whether cofilin-1 is associated with acute kidney injury (AKI) using human urine samples. We exploited a 96-well based high-throughput biosensor that uses gold nanoparticles and a sandwich immunoassay to detect the urine cofilin-1 level of AKI patients. The mean urine cofilin-1 level of the AKI patients (n=37 from 47 cases analyzed) was twofold higher than that of healthy adults (n=21 from 29 cases analyzed). The receiver operating characteristic (ROC) curve showed that cofilin-1 was acceptable for discriminating AKI patients from healthy adults. However, an increase of the sample size is required to conclude the importance of urine cofilin-1 on AKI diagnosis, and the high-throughput ultrasensitive biosensor used in this study would greatly accelerate the measurement of urine cofilin-1 in an increased sample size.
authors.affiliation: Chang Gung University, Molecular Medicine Research Center, Taoyuan 33302, Taiwan.
authors.forename: Ying-Feng
authors.initials: YF
authors.lastname: Chang
authors.forename: Cheng-Han
authors.initials: CH
authors.lastname: Chao
authors.forename: Lih-Yuan
authors.initials: LY
authors.lastname: Lin
authors.forename: Cheng-Han
authors.initials: CH
authors.lastname: Tsai
authors.forename: Chien
authors.initials: C
authors.lastname: Chou
authors.forename: Yi-Jang
authors.initials: YJ
authors.lastname: Lee
mesh_terms: D058186:Acute Kidney Injury / Q000652:urine*; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015374:Biosensing Techniques / Q000379:methods; D016022:Case-Control Studies; D002460:Cell Line; D051338:Cofilin 1 / Q000652:urine*; D005260:Female; D006046:Gold / Q000737:chemistry; D057166:High-Throughput Screening Assays / Q000379:methods*; D006801:Humans; D008297:Male; D053768:Metal Nanoparticles / Q000737:chemistry; D008875:Middle Aged; D018384:Oxidative Stress; D012372:ROC Curve; D013050:Spectrometry, Fluorescence / Q000379:methods*; D020349:Surface Plasmon Resonance / Q000379:methods*
chemical_list: D051338:Cofilin 1; D006046:Gold