title: Effect of β-carotene on catechol-induced genotoxicity in vitro: evidence of both enhanced and reduced DNA damage.
abstract: Intake of antioxidants from the diet has been recognized to have beneficial health effects, but the potential benefit of taking antioxidants such as β-carotene as supplements is controversial. The aim of the present study was to evaluate the potential protective effects of a physiologically relevant concentration (2 μM) of β-carotene on the DNA damaging effects of catechol in mouse lymphoma L5178Y cells. Two different exposure protocols were used: simultaneous exposure to β-carotene and catechol for 3 h; and exposure to catechol for 3 h after 18 h pre-treatment with the vitamin. DNA damage was evaluated using the comet assay (employing one procedure for general damage, and another procedure, which also included oxidative DNA damage). Independent of exposure protocol and procedure for comet assay, β-carotene did not increase the basal level of DNA damage. However, at the highest concentration of catechol (1 mM), β-carotene was found to clearly increase the level of catechol-induced DNA damage, especially in the pre-treated cells. Interestingly, an opposite effect was observed at lower concentrations of catechol, but the β-carotene related reduction of catechol-induced genotoxicity was significant (P < 0.05) only for the procedure including oxidative damage induced by 0.5 mM catechol. Taken together our results indicate that β- carotene can both reduce and enhance the DNA damaging effects of a genotoxic agent such as catechol. This indicates that it is the level of catechol-induced DNA damage that seems to determine whether β-carotene should be regarded as a beneficial or detrimental agent when it comes to its use as a dietary supplement.
authors.affiliation: Division of Toxicology, Department of Pharmaceutical Biosciences, Uppsala University, Uppsala, Sweden.
authors.forename: R
authors.initials: R
authors.lastname: Åsgård
authors.forename: B
authors.initials: B
authors.lastname: Hellman
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002396:Catechols / Q000494:pharmacology; D004249:DNA Damage / Q000187:drug effects*; D019587:Dietary Supplements; D006801:Humans; D007940:Leukemia L5178; D051379:Mice; D009152:Mutagenicity Tests; D018384:Oxidative Stress; D019207:beta Carotene / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D002396:Catechols; D019207:beta Carotene; C034221:catechol