title: Investigations into acrylamide precursors in sterilized table olives: evidence of a peptic fraction being responsible for acrylamide formation.
abstract: Formation of acrylamide from commercial model peptides containing protein-bound aspartic acid, alanine and methionine, respectively, at 200°C and different times in the absence of any carbonyl sources, was demonstrated by HPLC-MS/MS analyses. Further experiments using a more complex model system based on olive water, i.e., the aqueous fraction of olive pulp from untreated and lye-treated green olives, were performed. After partial fractionation of olive water by solid-phase extraction, only peptides/proteins containing fractions, being devoid of free asparagine, generated significant amounts of acrylamide during less harsh heat treatment (121°C for 30min). In contrast, acrylamide was not detected after heating the same fraction under identical thermal conditions when previously subjected to acid hydrolysis. Consistently, significant amounts of acrylamide were released after heating the albuminous precipitate resulting from acetone precipitation of olive water. These results strongly support the role of peptides/proteins as precursors of acrylamide formation in sterilized olives.
authors.affiliation: Institute of Food Science and Biotechnology, Hohenheim University, Garbenstrasse 25, D-70593 Stuttgart, Germany. javierhebrard@ig.csic.es
authors.forename: Francisco Javier
authors.initials: FJ
authors.lastname: Casado
authors.forename: Alfredo
authors.initials: A
authors.lastname: Montaño
authors.forename: Dietrich
authors.initials: D
authors.lastname: Spitzner
authors.forename: Reinhold
authors.initials: R
authors.lastname: Carle
mesh_terms: D020106:Acrylamide / Q000737:chemistry*; D003296:Cooking; D005511:Food Handling; D015416:Maillard Reaction; D031658:Olea / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry*; D010940:Plant Proteins / Q000737:chemistry*
chemical_list: D010455:Peptides; D010940:Plant Proteins; D020106:Acrylamide