title: Use of PCR to estimate the prevalence of Equus caballus papillomavirus in aural plaques in horses.
abstract: Aural plaques occur on the skin of the medial surface of the pinnae of horses. In this study the presence of Equus caballus papillomavirus (EcPV)-3 and -4 DNA was assessed in 45 such plaques using a 'touchdown' PCR. Papillomaviruses (PVs) were detected in 62.3% (28/45) of samples: EcPV-3 and -4 DNA in 8.89% (4/45) and 37.78% (17/45) of samples, respectively, with 15.56% (7/45) of samples exhibiting co-infection. Viral DNA was not detected in 37.78% (17/45) of samples, suggesting the possible existence of other equine PVs. Neither EcPV-3 nor -4 were detected in negative control skin. This study is the first to evaluate the prevalence of these two viruses in equine aural plaques.
authors.affiliation: Department of Veterinary Clinical Science, College of Veterinary Medicine and Animal Science, Univ. Estadual Paulista (UNESP), Botucatu, SP 18618970, Brazil.
authors.forename: Ana Claudia
authors.initials: AC
authors.lastname: Gorino
authors.forename: Jose P
authors.initials: JP
authors.lastname: Oliveira-Filho
authors.forename: Sueli A
authors.initials: SA
authors.lastname: Taniwaki
authors.forename: Roberta M
authors.initials: RM
authors.lastname: Basso
authors.forename: Luiza S
authors.initials: LS
authors.lastname: Zakia
authors.forename: João P
authors.initials: JP
authors.lastname: Araujo
authors.forename: Alexandre S
authors.initials: AS
authors.lastname: Borges
mesh_terms: D000818:Animals; D004279:DNA, Viral / Q000235:genetics; D004427:Ear Diseases / Q000662:veterinary* / Q000821:virology; D006734:Horse Diseases / Q000175:diagnosis / Q000821:virology*; D006736:Horses; D027383:Papillomaviridae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D030361:Papillomavirus Infections / Q000175:diagnosis / Q000662:veterinary* / Q000821:virology; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary*; D012680:Sensitivity and Specificity; D017193:Skin Diseases, Viral / Q000175:diagnosis / Q000662:veterinary*
chemical_list: D004279:DNA, Viral
keywords: Aural plaques; Horse; PCR; Papillomavirus