title: Quantification of major urinary metabolites of PGE2 and PGD2 in cystic fibrosis: correlation with disease severity.
abstract: Cystic fibrosis transmembrane conductance (CFTR) alterations are involved in the overproduction of prostaglandins (PG) in CF in vitro. We assessed the relationship between PGE-M and PGD-M urinary metabolites of PGE2 and PGD2 and CF severity. Twenty-four controls and 35 CF patients were recruited. PGE-M and PGD-M levels were measured by liquid chromatography/mass spectrometry and results were expressed as median and 25th-75th interquartile of ng/mg creatinine (Cr). PGE-M (15.63; 9.07-43.35ng/mg Cr) and PGD-M (2.16; 1.43-3.53ng/mg Cr) concentrations were higher in CF than in controls: PGE-M, (6.63; 4.35-8.60ng/mg Cr); PGD-M (1.23; 0.96-1.54ng/mg Cr). There was no correlation between metabolite levels and spirometric values. Patients with pancreatic insufficiency (n=29) had higher PGE-M levels (19.09; 9.36-52.69ng/mg Cr) than those with conserved function (n=6) (9.61; 5.78-14.34ng/mg Cr). PGE-M levels were associated with genotype severity: mild (7.14; 5.76-8.76, n=8), moderate (16.67; 13.67-28.62ng/mg Cr, n=5) and severe (22.82; 10.67-84.13ng/mg Cr). Our study confirms the key role of CFTR in the regulation of the cyclooxygenase pathway of arachidonic acid metabolism found in in vitro studies.
authors.affiliation: Servei de Pneumologia, Hospital Cinic, Institut d'Investigacions Biomèdiques August PI i Sunyer (IDIBAPS), CIBERES, Universitat de Barcelona, 08036 Barcelona, Spain.
authors.forename: Suha
authors.initials: S
authors.lastname: Jabr
authors.forename: Silvia
authors.initials: S
authors.lastname: Gartner
authors.forename: Ginger L
authors.initials: GL
authors.lastname: Milne
authors.forename: Jordi
authors.initials: J
authors.lastname: Roca-Ferrer
authors.forename: Josefina
authors.initials: J
authors.lastname: Casas
authors.forename: Antonio
authors.initials: A
authors.lastname: Moreno
authors.forename: Emilio
authors.initials: E
authors.lastname: Gelpí
authors.forename: César
authors.initials: C
authors.lastname: Picado
mesh_terms: D000293:Adolescent; D002648:Child; D003550:Cystic Fibrosis / Q000378:metabolism / Q000473:pathology* / Q000652:urine*; D015232:Dinoprostone / Q000378:metabolism* / Q000652:urine*; D005260:Female; D006801:Humans; D008297:Male; D015230:Prostaglandin D2 / Q000378:metabolism* / Q000652:urine*
chemical_list: D015232:Dinoprostone; D015230:Prostaglandin D2
keywords: Arachidonic acid; Cyclooxygenase; Cystic fibrosis; Prostaglandins; Tetranor PGD-M; Tetranor PGE-M