title: Functional study of diacylglycerol acyltransferase type 2 family in Chlamydomonas reinhardtii.
abstract: Algal triacylglycerol biosynthesis is of increasing interest for potential biodiesel production. A model microalga, Chlamydomonas, has multiple isoforms of diacylglycerol acyltransferase type 2 (DGTT) catalyzing the final step of triacylglycerol biosynthesis; however, the functions of the isoforms are poorly understood. Here, we performed heterologous complementation assay of Chlamydomonas DGTT1 to 4 in a yeast mutant defective in triacylglycerol biosynthesis. DGTT1, 2 and 3 but not 4 complemented the phenotype, including triacylglycerol levels. Interestingly, complementation by DGTT2 increased triacylglycerol content by 9-fold.
authors.affiliation: Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan.
authors.forename: Chun-Hsien
authors.initials: CH
authors.lastname: Hung
authors.forename: Ming-Yang
authors.initials: MY
authors.lastname: Ho
authors.forename: Kazue
authors.initials: K
authors.lastname: Kanehara
authors.forename: Yuki
authors.initials: Y
authors.lastname: Nakamura
mesh_terms: D000595:Amino Acid Sequence; D016825:Chlamydomonas reinhardtii / Q000201:enzymology*; D051048:Diacylglycerol O-Acyltransferase / Q000737:chemistry / Q000378:metabolism*; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid
chemical_list: D051048:Diacylglycerol O-Acyltransferase
keywords: Algal biodiesel; Bioenergy; Chlamydomonas reinhardtii; DGAT; DGTT; Diacylglycerol acyltransferase; Lipid droplet; PDAT; TAG; Triacylglycerol; diacylglycerol acyltransferase type 2; phospholipid:diacylglycerol acyltransferase; sn-1,2-diacylglycerol acyltransferase; triacylglycerol