title: Differential expression of STAT1 and IFN-γ in primary and invasive or metastatic wilms tumors.
abstract: OBJECTIVE
  IFN/STAT1 signaling has been found to be not only associated with an aggressive tumor phenotype but also activated and functional during metanephric development. This study was undertaken to evaluate STAT1 and IFN-γ expression and its relation to histopathological features of primary and invasive/metastatic Wilms tumors.
  
  
  METHODS
  Immunohistochemistry was used to determine the expression and cellular distribution of STAT1 and IFN-γ in 18 pairs of primary and corresponding invasive/metastatic Wilms tumors and 40 primary tumors without invasion or metastasis.
  
  
  RESULTS
  Positive rate of STAT1/IFN-γ expression was 66.7%/61.1% and 72.2%/77.8% in 18 pairs of primary and associated invasive/metastatic Wilms tumor tissues, while 35.0%/27.5% in 40 primary tumors without invasion or metastasis. The expression of STAT1 and IFN-γ was significantly associated with invasion/metastasis (P = 0.025; P = 0.015). There was a positive correlation between STAT1 and IFN-γ expression in all Wilms tumor tissues (χ(2)  = 23.408, P = 0.05, r = 0.555). The expression of STAT1 and IFN-γ between primary and matched invasive/metastatic tissues was concordance, respectively (P = 0.710 and P = 0.375).
  
  
  CONCLUSIONS
  These results suggest that IFN-γ/STAT1 signaling might have clinical potential as a promising predictor to identify individuals with poor prognostic potential and as a possible novel target molecule of therapy for Wilms tumor.
authors.affiliation: Department of Pediatric Surgery, Provincial Hospital Affiliated to Shandong University, Jinan, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.forename: Lijuan
authors.initials: L
authors.lastname: Zhang
authors.forename: Rongde
authors.initials: R
authors.lastname: Wu
mesh_terms: D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007223:Infant; D007371:Interferon-gamma / Q000032:analysis* / Q000502:physiology; D007680:Kidney Neoplasms / Q000737:chemistry* / Q000473:pathology; D008297:Male; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D050794:STAT1 Transcription Factor / Q000032:analysis* / Q000502:physiology; D015398:Signal Transduction; D009396:Wilms Tumor / Q000737:chemistry* / Q000473:pathology
chemical_list: D050794:STAT1 Transcription Factor; C494079:STAT1 protein, human; D007371:Interferon-gamma
keywords: IFN-γ; STAT1; Wilms tumor; invasion; metastasis