title: O-glycan profiling of serum glycan for potential renal cancer biomarkers.
abstract: Serum was obtained from 25 male renal cell carcinoma (RCC) patients and 21 healthy males. O-glycans were released by a β-elimination reaction and purified by graphitized carbon cartridge solid phase extraction, then profiled by matrix-assisted laser desorption/ionisation-time of flight mass spectrometry. After noise removal and peak alignment, 1372 peaks were extracted from 200000 data points. Feature peaks were analyzed by calculation of differential sensitivity and specificity. The combination of two feature peaks was chosen as a biomarker and could clearly differentiate RCC and normal samples in our study group.
authors.affiliation: Department of Urology, Changzheng Hospital of Second Military Medical University, Shanghai 200003, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Gao
authors.forename: Fei
authors.initials: F
authors.lastname: Ma
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: Fan
authors.initials: F
authors.lastname: Zhong
authors.forename: HaiLin
authors.initials: H
authors.lastname: Tang
authors.forename: DanFeng
authors.initials: D
authors.lastname: Xu
authors.forename: Liang
authors.initials: L
authors.lastname: Zhao
mesh_terms: D014408:Biomarkers, Tumor / Q000097:blood*; D002292:Carcinoma, Renal Cell / Q000097:blood*; D016022:Case-Control Studies; D054794:Glycomics / Q000379:methods; D006801:Humans; D007680:Kidney Neoplasms / Q000097:blood*; D008297:Male; D011134:Polysaccharides / Q000097:blood*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D014408:Biomarkers, Tumor; D011134:Polysaccharides