title: Anti-inflammatory components from the root of Solanum erianthum.
abstract: Two new norsesquiterpenoids, solanerianones A and B (1-2), together with nine known compounds, including four sesquiterpenoids, (-)-solavetivone (3), (+)-anhydro-β-rotunol (4), solafuranone (5), lycifuranone A (6); one alkaloid, N-trans-feruloyltyramine (7); one fatty acid, palmitic acid (8); one phenylalkanoid, acetovanillone (9), and two steroids, β-sitosterol (10) and stigmasterol (11) were isolated from the n-hexane-soluble part of the roots of Solanum erianthum. Their structures were elucidated on the basis of physical and spectroscopic data analyses. The anti-inflammatory activity of these isolates was monitored by nitric oxide (NO) production in lipopolysaccharide (LPS)-activated murine macrophage RAW264.7 cells. The cytotoxicity towards human lung squamous carcinoma (CH27), human hepatocellular carcinoma (Hep 3B), human oral squamous carcinoma (HSC-3) and human melanoma (M21) cell lines was also screened by using an MTT assay. Of the compounds tested, 3 exhibited the strongest NO inhibition with the average maximum inhibition (Emax) at 100 μM and median inhibitory concentration (IC50) values of 98.23% ± 0.08% and 65.54 ± 0.18 μM, respectively. None of compounds (1-9) was found to possess cytotoxic activity against human cancer cell lines at concentrations up to 30 μM.
authors.affiliation: Department of Chinese Pharmaceutical Sciences and Chinese Medicine Resources, College of Pharmacy, China Medical University, Taichung 404, Taiwan. yuchang@mail.cmu.edu.tw.
authors.forename: Yu-Chang
authors.initials: YC
authors.lastname: Chen
authors.forename: Hong-Zin
authors.initials: HZ
authors.lastname: Lee
authors.forename: Hsin-Chun
authors.initials: HC
authors.lastname: Chen
authors.forename: Chi-Luan
authors.initials: CL
authors.lastname: Wen
authors.forename: Yueh-Hsiung
authors.initials: YH
authors.lastname: Kuo
authors.forename: Guei-Jane
authors.initials: GJ
authors.lastname: Wang
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D066241:Carbon-13 Magnetic Resonance Spectroscopy; D016923:Cell Death / Q000187:drug effects; D045744:Cell Line, Tumor; D006801:Humans; D020128:Inhibitory Concentration 50; D008070:Lipopolysaccharides / Q000494:pharmacology; D051379:Mice; D018517:Plant Roots / Q000737:chemistry*; D066244:Proton Magnetic Resonance Spectroscopy; D032322:Solanum / Q000737:chemistry*
chemical_list: D000893:Anti-Inflammatory Agents; D008070:Lipopolysaccharides