title: Next-generation sequencing of Okazaki fragments extracted from Saccharomyces cerevisiae.
abstract: Genome-wide Okazaki fragment distribution can differentiate the discontinuous from the semi-discontinuous DNA replication model. Here, we investigated the genome-wide Okazaki fragment distribution in Saccharomyces cerevisiae S288C. We improved the method based upon lambda exonuclease digestion to purify Okazaki fragments from S288C yeast cells, followed by Illumina sequencing. The distribution of Okazaki fragments around confirmed replication origins, including two highly efficient replication origins, supported the discontinuous DNA replication model. In S. cerevisiae mitochondria, Okazaki fragments were overrepresented in the transcribed regions, indicating the interplay between transcription and DNA replication.
authors.affiliation: School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, PR China. chaosyw@126.com
authors.forename: Wenchao
authors.initials: W
authors.lastname: Yanga
authors.forename: Xinhui
authors.initials: X
authors.lastname: Lib
mesh_terms: D015825:Chromosomes, Fungal; D004247:DNA / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics*
chemical_list: C022262:Okazaki fragments; D004247:DNA
keywords: Budding yeast; DNA replication; Lambda exonuclease digestion; Okazaki fragment