title: Low oxygen tension is critical for the culture of human mesenchymal stem cells with strong osteogenic potential from haemarthrosis fluid.
abstract: Satisfactory osseous tissue integration of the soft tissue graft with bone is the mainstay of healing following surgical reconstruction of the anterior cruciate ligament (ACL). However, tissue remodelling is slow and significantly impacts on quality of life by delaying return to work and sport and accelerating the onset of degenerative diseases such as osteoarthritis. Delivery of multipotent human mesenchymal stem cells (hMSCs) at surgery could enhance osseous tissue integration. We aim to use hMSCs derived from haemarthrosis fluid (HF) (the intra-articular bleed accrued post-trauma) which is aspirated and discarded as clinical waste. With the aim of improving our bioprocessing methodologies for clinical translation we have investigated the effect of low oxygen tension on the derivation and osteogenic potential of this novel HF-hMSC population. Mononuclear cells were isolated from HF aspirated samples and divided for derivation and culture under normal or low oxygen tension. HF-hMSCs were derived from 100 % of cultures under low oxygen tension compared to 71 % for normal oxygen tension; this was coupled with increased CFU-Fs. We investigated the osteogenic potential and cellular health of HF-hMSC populations following ex vivo expansion. HF-hMSC populations showed enhanced matrix mineralisation and cellular health when differentiated under low oxygen tension. This positive effect of low oxygen on osteogenesis and cellular health was reduced with prolonged culture. These data demonstrate that derivation and culture of HF-hMSC populations under low oxygen tension will enable the translation of a cellular therapy for the treatment of broad patient numbers with optimal osteogenic potency and cellular vitality.
authors.affiliation: Institute of Cellular Medicine, Faculty of Medical Sciences, Newcastle University, International Centre for Life, Times Square, Newcastle-upon-Tyne, NE1 4EP, UK.
authors.forename: Callie A
authors.initials: CA
authors.lastname: Knuth
authors.forename: Marcia E
authors.initials: ME
authors.lastname: Clark
authors.forename: Annette P
authors.initials: AP
authors.lastname: Meeson
authors.forename: Sameer K
authors.initials: SK
authors.lastname: Khan
authors.forename: Daniel J
authors.initials: DJ
authors.lastname: Dowen
authors.forename: David J
authors.initials: DJ
authors.lastname: Deehan
authors.forename: Rachel A
authors.initials: RA
authors.lastname: Oldershaw
mesh_terms: D015703:Antigens, CD / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis; D004305:Dose-Response Relationship, Drug; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D005434:Flow Cytometry; D006395:Hemarthrosis / Q000097:blood / Q000473:pathology; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000378:metabolism; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D010012:Osteogenesis / Q000187:drug effects*; D010100:Oxygen / Q000378:metabolism / Q000494:pharmacology*; D013234:Stem Cells; D013997:Time Factors
chemical_list: D015703:Antigens, CD; D004247:DNA; D010100:Oxygen