title: Matrix metalloproteinases (MMPs) inhibitory effects of an octameric oligopeptide isolated from abalone Haliotis discus hannai.
abstract: Abalone (Haliotis discus hannai) is a marine gastropod, and an important fishery and food industrial resource that is massively maricultured in Asia, Africa, Australia and America. However, its health benefits have rarely been studied for nutraceutical and pharmaceutical application. In this study, the purified abalone oligopeptide (AOP) with anti-matrix metalloproteinases (anti-MMPs) effects was isolated from the digests of abalone intestine using recycle HPLC with a JAI W253 column and an OHpak SB-803 HQ column. The AOP was identified as Ala-Glu-Leu-Pro-Ser-Leu-Pro-Gly (MW=782.4 Da) with a de novo peptide sequencing technique using a tandem mass spectrometer. The AOP exhibited a specific inhibitory effect against MMP-2/-9 activity and attenuated protein expression of p50 and p65 in the human fibrosarcoma (HT1080) cells, dose-dependently. The results presented illustrate that the AOP could inhibit MMP-2/-9 expression in HT1080 cells via the nuclear factor-kappaB (NF-κB)-mediated pathway. This data suggest that the AOP from H. discus hannai intestine may possess therapeutic and preventive potential for the treatment of MMPs-related disorders such as angiogenesis and cardiovascular diseases.
authors.affiliation: Department of Marine Life Science and Marine Life Research & Education Center, Chosun University, Gwangju 501-759, Republic of Korea.
authors.forename: Van-Tinh
authors.initials: VT
authors.lastname: Nguyen
authors.forename: Zhong-Ji
authors.initials: ZJ
authors.lastname: Qian
authors.forename: Bomi
authors.initials: B
authors.lastname: Ryu
authors.forename: Kil-Nam
authors.initials: KN
authors.lastname: Kim
authors.forename: Daekyung
authors.initials: D
authors.lastname: Kim
authors.forename: Young-Mog
authors.initials: YM
authors.lastname: Kim
authors.forename: You-Jin
authors.initials: YJ
authors.lastname: Jeon
authors.forename: Won Sun
authors.initials: WS
authors.lastname: Park
authors.forename: Il-Whan
authors.initials: IW
authors.lastname: Choi
authors.forename: Geun Hyung
authors.initials: GH
authors.lastname: Kim
authors.forename: Jae-Young
authors.initials: JY
authors.lastname: Je
authors.forename: Won-Kyo
authors.initials: WK
authors.lastname: Jung
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D041981:Gastrointestinal Tract / Q000737:chemistry; D049851:Gastropoda / Q000737:chemistry*; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D061965:Matrix Metalloproteinase Inhibitors / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D020782:Matrix Metalloproteinases / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology
chemical_list: D061965:Matrix Metalloproteinase Inhibitors; D009842:Oligopeptides; D020782:Matrix Metalloproteinases