title: Synthesis of SiO2 system via sol-gel process: biocompatibility tests with a fibroblast strain and release kinetics.
abstract: SiO2 glass has been synthesized via sol-gel process and enriched with 5 wt % sodium ampicillin. To verify the biocompatibility of the obtained biomaterial, fibroblasts have been grown on a glass surface and were tested for viability after 24 h. The results of the Water-Soluble Tetrazolium (WST)-8 analysis suggest that SiO2 glass has an adequate biocompatibility. The amorphous nature of the gels has been ascertained by X-ray diffraction analysis. Release kinetics have been subsequently investigated in a simulated body fluid. The amount of sodium ampicillin released has been detected by ultraviolet-visible spectroscopy. The release kinetics seems to occur in more than one stage. High-performance liquid chromatography analysis has also been carried out to ensure the integrity of ampicillin after the synthesis treatment.
authors.affiliation: Department of Industrial and Information Engineering, Second University of Naples, 81031, Aversa, Italy.
authors.forename: Michelina
authors.initials: M
authors.lastname: Catauro
authors.forename: Flavia
authors.initials: F
authors.lastname: Bollino
authors.forename: Ferdinando
authors.initials: F
authors.lastname: Papale
mesh_terms: D016475:3T3 Cells; D000667:Ampicillin / Q000008:administration & dosage*; D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage*; D001672:Biocompatible Materials / Q000138:chemical synthesis* / Q000737:chemistry; D002470:Cell Survival; D004337:Drug Carriers / Q000138:chemical synthesis* / Q000737:chemistry; D005347:Fibroblasts / Q000166:cytology*; D008422:Materials Testing; D051379:Mice; D044367:Phase Transition; D012822:Silicon Dioxide / Q000138:chemical synthesis* / Q000737:chemistry
chemical_list: D000900:Anti-Bacterial Agents; D001672:Biocompatible Materials; D004337:Drug Carriers; D012822:Silicon Dioxide; D000667:Ampicillin
keywords: biocompatibility; bioglasses; drug release; sol-gel