title: Optical developments for optogenetics.
abstract: Brain intricacies and the difficulty that scientists encounter in revealing its function with standard approaches such as electrical stimulation of neurons have led to the exploration of new tools that enable the study of neural circuits in a remote and non-invasive way. To this end, optogenetics has initialised a revolution for neuroscience in the last decade by enabling simultaneous monitoring and stimulation of specific neuronal populations in intact brain preparations through genetically targeted expression of light sensitive proteins and molecular photoswitches. In addition to ongoing molecular probe development and optimisation, novel optical techniques hold immense potential to amplify and diversify the utility of optogenetic methods. Importantly, by improving the spatio-temporal resolution of light stimulation, neural circuits can be photoactivated in patterns mimicking endogenous physiological processes. The following synopsis addresses the possibilities and limitations of optical stimulation methods applied to and developed for activation of neuronal optogenetic tools.
authors.affiliation: Wavefront-Engineering Microscopy Group, Neurophysiology and New Microscopies Laboratory, CNRS UMR 8154, Inserm S603, Paris Descartes University, 75270 Paris Cedex 06, France.
authors.forename: Eirini
authors.initials: E
authors.lastname: Papagiakoumou
mesh_terms: D000818:Animals; D006801:Humans; D009474:Neurons / Q000166:cytology / Q000378:metabolism / Q000528:radiation effects; D061848:Optical Imaging / Q000379:methods*; D062308:Optogenetics / Q000295:instrumentation* / Q000379:methods*
keywords: Digital holography; Generalised phase contrast; Illumination methods; Optogenetics; Spatio-temporal focusing