title: Dynamic protein phosphorylation during the growth of Xanthomonas campestris pv. campestris B100 revealed by a gel-based proteomics approach.
abstract: Xanthomonas campestris pv. campestris (Xcc) synthesizes huge amounts of the exopolysaccharide xanthan and is a plant pathogen affecting Brassicaceae, among them the model plant Arabidopsis thaliana. Xanthan is produced as a thickening agent at industrial scale by fermentation of Xcc. In an approach based on 2D gel electrophoresis, protein samples from different growth phases were characterized to initialize analysis of the Xanthomonas phosphoproteome. The 2D gels were stained with Pro-Q Diamond phosphoprotein stain to identify putatively phosphorylated proteins. Spots of putatively phosphorylated proteins were excised from the gel and analyzed by mass spectrometry. Three proteins were confirmed to be phosphorylated, the phosphoglucomutase/phosphomannomutase XanA that is important for xanthan and lipopolysaccharide biosynthesis, the phosphoenolpyruvate synthase PspA that is involved in gluconeogenesis, and an anti-sigma factor antagonist RsbR that was so far uncharacterized in xanthomonads. The growth phase in which the samples were collected had an influence on protein phosphorylation in Xcc, particular distinct in case of RsbR, which was phosphorylated during the transition from the late exponential growth phase to the stationary phase.
authors.affiliation: Department of Proteome and Metabolome Research, Centre for Biotechnology & Faculty of Biology, Bielefeld University, Universitätsstr. 25, 33615 Bielefeld, Germany.
authors.forename: Yaarub Raji
authors.initials: YR
authors.lastname: Musa
authors.forename: Katrin
authors.initials: K
authors.lastname: Bäsell
authors.forename: Sarah
authors.initials: S
authors.lastname: Schatschneider
authors.forename: Frank-Jörg
authors.initials: FJ
authors.lastname: Vorhölter
authors.forename: Dörte
authors.initials: D
authors.lastname: Becher
authors.forename: Karsten
authors.initials: K
authors.lastname: Niehaus
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D005943:Gluconeogenesis / Q000502:physiology; D008070:Lipopolysaccharides / Q000378:metabolism; D008969:Molecular Sequence Data; D010733:Phosphoglucomutase / Q000378:metabolism; D010766:Phosphorylation; D017866:Phosphotransferases (Paired Acceptors) / Q000378:metabolism; D017875:Phosphotransferases (Phosphomutases) / Q000378:metabolism; D011135:Polysaccharides, Bacterial / Q000378:metabolism; D040901:Proteomics / Q000379:methods*; D016959:Xanthomonas campestris / Q000254:growth & development / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D008070:Lipopolysaccharides; D011135:Polysaccharides, Bacterial; D017866:Phosphotransferases (Paired Acceptors); C022509:pyruvate, water dikinase; D017875:Phosphotransferases (Phosphomutases); D010733:Phosphoglucomutase; C052799:phosphomannomutase; C002563:xanthan gum
keywords: Anti-anti-sigma factor; Bacteria; EPS; LPS; Posttranslational modification