title: One year survey of human rotavirus strains suggests the emergence of genotype G12 in Cameroon.
abstract: In this study the emergence of rotavirus A genotype G12 in children <5 years of age is reported from Cameroon during 2010/2011. A total of 135 human stool samples were P and G genotyped by reverse transcriptase PCR. Six different rotavirus VP7 genotypes were detected, including G1, G2, G3, G8, G9, and G12 in combinations with P[4], P[6] and P[8] VP4 genotypes. Genotype G12 predominated in combination with P[8] (54.1%) and P[6] (10.4%) genotypes followed by G1P[6] (8.2%), G3P[6] (6.7%), G2P[4] (5.9%), G8P[6] (3.7%), G2P[6] (0.7%), G3P[8] (0.7%), and G9P[8] (0.7%). Genotype P[6] strains in combination with various G-types represented a substantial proportion (N=44, 32.6%) of the genotyped strains. Partially typed strains included G12P[NT] (2.2%); G3P[NT] (0.7%); G(NT)P[6] (1.5%); and G(NT)P[8] (0.7%). Mixed infections were found in five specimens (3.7%) in several combinations including G1+ G12P[6], G2+ G3P[6] + P[8], G3+ G8P[6], G3 + G12P[6] + P[8], and G12P[6] +P[8]. The approximately 10% relative frequency of G12P[6] strains detected in this study suggests that this strain is emerging in Cameroon and should be monitored carefully as rotavirus vaccine is implemented in this country, as it shares neither G- nor P-type specificity with strains in the RotaTeq® and Rotarix® vaccines. These findings are consistent with other recent reports of the global spread and increasing epidemiologic importance of G12 and P[6] strains.
authors.affiliation: University of Yaoundé I, Yaoundé, Cameroon.
authors.forename: Valentine N
authors.initials: VN
authors.lastname: Ndze
authors.forename: Hajnalka
authors.initials: H
authors.lastname: Papp
authors.forename: Eric A
authors.initials: EA
authors.lastname: Achidi
authors.forename: Kamga H
authors.initials: KH
authors.lastname: Gonsu
authors.forename: Brigitta
authors.initials: B
authors.lastname: László
authors.forename: Szilvia
authors.initials: S
authors.lastname: Farkas
authors.forename: Péter
authors.initials: P
authors.lastname: Kisfali
authors.forename: Béla
authors.initials: B
authors.lastname: Melegh
authors.forename: Mathew D
authors.initials: MD
authors.lastname: Esona
authors.forename: Michael D
authors.initials: MD
authors.lastname: Bowen
authors.forename: K
authors.initials: K
authors.lastname: Bányai
authors.forename: Jon R
authors.initials: JR
authors.lastname: Gentsch
authors.forename: Abena M T
authors.initials: AM
authors.lastname: Odama
mesh_terms: D002163:Cameroon / Q000453:epidemiology; D002675:Child, Preschool; D060085:Coinfection / Q000453:epidemiology / Q000821:virology; D005243:Feces / Q000821:virology; D005838:Genotype; D006801:Humans; D007223:Infant; D017720:Molecular Epidemiology; D012367:RNA, Viral / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012401:Rotavirus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D012400:Rotavirus Infections / Q000453:epidemiology* / Q000821:virology*
chemical_list: D012367:RNA, Viral