title: Donor body mass index is an important factor that affects peripheral blood progenitor cell yield in healthy donors after mobilization with granulocyte-colony-stimulating factor.
abstract: BACKGROUND
  The use of hematopoietic progenitor cell (HPC) transplantation has rapidly expanded in recent years. Currently, several sources of HPCs are available for transplantation including peripheral blood HPCs (PBPCs), cord blood cells, and marrow cells. Of these, PBPC collection has become the major source of HPCs. An important variable in PBPC collection is the response to PBPC mobilization, which varies significantly and sometime causes mobilization failure.
  
  
  METHODS
  A retrospective study of 69 healthy donors who underwent PBPC donation by leukapheresis was performed. All of these donors received 10 μg/kg/day or more granulocyte-colony-stimulating factor (G-CSF) for 5 days before PBPC harvest. Donor factors were evaluated and correlated with mobilization responses, as indicated by the precollection CD34 count (pre-CD34).
  
  
  RESULTS
  Donors with a pre-CD34 of more than 100 × 10(6) /L had higher body mass index (BMI) compared with donors whose pre-CD34 was 38 × 10(6) to 99 × 10(6) /L or less than 38 × 10(6) /L (32.0 ± 1.04 kg/m(2) vs. 28.7 ± 0.93 kg/m(2) vs. 25.9 ± 1.27 kg/m(2) , respectively; p < 0.05). In addition, donors with high BMIs had higher pre-CD34 on a per-kilogram-of-body-weight basis compared with donors with low BMIs.
  
  
  CONCLUSIONS
  BMI is an important factor that affects donor's response to mobilization and consequently the HPC yield. This effect may be due to a relatively high dose of G-CSF administered to donors with higher BMI or due to the presence of unknown intrinsic factors affecting mobilization that correlate with the amount of adipose tissue in each donor.
authors.affiliation: Department of Pathology and Genomic Medicine, Cell and Gene Therapy Department, Baylor College of Medicine, Houston, Texas; The Methodist Hospital, Houston, Texas; Gulf Coast Regional Blood Center, Houston, Texas.
authors.forename: Jian
authors.initials: J
authors.lastname: Chen
authors.forename: Kevin M
authors.initials: KM
authors.lastname: Burns
authors.forename: Aleksandar
authors.initials: A
authors.lastname: Babic
authors.forename: George
authors.initials: G
authors.lastname: Carrum
authors.forename: Martha
authors.initials: M
authors.lastname: Kennedy
authors.forename: Francisco J
authors.initials: FJ
authors.lastname: Segura
authors.forename: Salvador
authors.initials: S
authors.lastname: Garcia
authors.forename: Sandra
authors.initials: S
authors.lastname: Potts
authors.forename: Christopher
authors.initials: C
authors.lastname: Leveque
mesh_terms: D000328:Adult; D001772:Blood Cell Count; D001782:Blood Donors; D015992:Body Mass Index; D002469:Cell Separation; D005260:Female; D016179:Granulocyte Colony-Stimulating Factor / Q000008:administration & dosage; D019650:Hematopoietic Stem Cell Mobilization / Q000379:methods*; D018380:Hematopoietic Stem Cell Transplantation; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000187:drug effects; D006801:Humans; D007937:Leukapheresis / Q000379:methods*; D008297:Male; D008875:Middle Aged; D012189:Retrospective Studies; D055815:Young Adult
chemical_list: D016179:Granulocyte Colony-Stimulating Factor