title: Characterization and complete genome sequence analysis of Staphylococcus aureus bacteriophage SA12.
abstract: Staphylococcus aureus is a well-known pathogen that causes several serious diseases in humans and animals. As part of the efforts to control this pathogen, a newly isolated bacteriophage, SA12, which specifically targets S. aureus, was characterized, and its genome was completely sequenced. Host range and bacteriophage challenge tests demonstrated its specific and efficient host lysis of S. aureus. The genome of phage SA12 consists of 42,902 bp length double-stranded DNA with 58 predicted ORFs-encoding phage structure, DNA manipulation, packaging, host lysis, and regulation proteins. The characterization and genome study of phage SA12 in this report is useful for understanding S. aureus-targeting bacteriophages and provides basic information for the further development of phage-based biocontrol agents against S. aureus.
authors.affiliation: Department of Food and Animal Biotechnology, Seoul National University, Seoul, 151-921, Korea.
authors.forename: Yoonjee
authors.initials: Y
authors.lastname: Chang
authors.forename: Ju-Hoon
authors.initials: JH
authors.lastname: Lee
authors.forename: Hakdong
authors.initials: H
authors.lastname: Shin
authors.forename: Sunggi
authors.initials: S
authors.lastname: Heu
authors.forename: Sangryeol
authors.initials: S
authors.lastname: Ryu
mesh_terms: D001433:Bacteriolysis; D004279:DNA, Viral / Q000737:chemistry* / Q000235:genetics*; D016679:Genome, Viral; D058507:Host Specificity; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D017422:Sequence Analysis, DNA; D013204:Staphylococcus Phages / Q000235:genetics* / Q000302:isolation & purification / Q000502:physiology; D013211:Staphylococcus aureus / Q000821:virology*; D014764:Viral Proteins / Q000235:genetics
chemical_list: D004279:DNA, Viral; D014764:Viral Proteins