title: Dipeptidyl peptidase-IV inhibitory peptides generated by tryptic hydrolysis of a whey protein concentrate rich in β-lactoglobulin.
abstract: Dipeptidyl peptidase-IV (DPP-IV) is a serine protease involved in the degradation and inactivation of incretin hormones that act by stimulating glucose-dependent insulin secretion after meal ingestion. DPP-IV inhibitors have emerged as new and promising oral agents for the treatment of type 2 diabetes. The purpose of this study was to investigate the potential of β-lactoglobulin as natural source of DPP-IV inhibitory peptides. A whey protein concentrate rich in β-lactoglobulin was hydrolysed with trypsin and fractionated using a chromatographic separation at semipreparative scale. Two of the six collected fractions showed notable DPP-IV inhibitory activity. These fractions were analysed by HPLC coupled to tandem mass spectrometry (HPLC-MS/MS) to identify peptides responsible for the observed activity. The most potent fragment (IPAVF) corresponded to β-lactoglobulin f(78-82) which IC50 value was 44.7μM. The results suggest that peptides derived from β-lactoglobulin would be beneficial ingredients of foods against type 2 diabetes.
authors.affiliation: Instituto de Investigación en Ciencias de la Alimentación (CIAL, CSIC-UAM, CEI UAM+CSIC), Nicolás Cabrera 9, 28049 Madrid, Spain.
authors.forename: Silvana T
authors.initials: ST
authors.lastname: Silveira
authors.forename: Daniel
authors.initials: D
authors.lastname: Martínez-Maqueda
authors.forename: Isidra
authors.initials: I
authors.lastname: Recio
authors.forename: Blanca
authors.initials: B
authors.lastname: Hernández-Ledesma
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D018819:Dipeptidyl Peptidase 4 / Q000032:analysis*; D054873:Dipeptidyl-Peptidase IV Inhibitors / Q000737:chemistry*; D006801:Humans; D006868:Hydrolysis; D007700:Kinetics; D007782:Lactoglobulins / Q000032:analysis*; D013058:Mass Spectrometry; D008894:Milk Proteins / Q000737:chemistry*; D008969:Molecular Sequence Data; D010449:Peptide Mapping; D011492:Protein Hydrolysates / Q000737:chemistry*; D000067816:Whey Proteins
chemical_list: D054873:Dipeptidyl-Peptidase IV Inhibitors; D007782:Lactoglobulins; D008894:Milk Proteins; D011492:Protein Hydrolysates; D000067816:Whey Proteins; C042807:DPP4 protein, human; D018819:Dipeptidyl Peptidase 4