title: Lung microRNA profile in chronic cyanotic piglets with decreased pulmonary blood flow.
abstract: BACKGROUND
  Cyanotic congenital heart defects with decreased pulmonary blood flow due to lung ischemia, hypoxia, and others lead to infant morbidity and mortality more than acyanotic heart disease does. Despite the great effort of medical research, their genetic link and underlying microRNAs molecular mechanisms remain obscure. In this study, we aimed to investigate microRNAs regulation during cyanotic defects in lung of immature piglets.
  
  
  METHODS
  Cyanotic piglet model was induced by main pulmonary artery-left atrium shunt with distal pulmonary artery banding. Four weeks later, hemodynamic parameters confirmed the development of cyanotic defects and pulmonary lobe RNA was extracted from all animals. We studied the repertoire of porcine lung microRNAs by Solexa deep sequencing technology and quantified highly expressed microRNAs by microarray hybridization. Furthermore, we quantitated selected microRNAs from cyanotic and control piglets by quantitative RT-PCR.
  
  
  RESULTS
  After surgical procedure 4 weeks later, the cyanotic model produced lower arterial oxygen tension, arterial oxygen saturation, and higher arterial carbon dioxide tension, hematocrit and hemoglobin concentration than controls (all P < 0.05). In 1273 miRNAs expressed in the immature piglets lungs, 2 most abundant microRNAs (miR-370 and miR-320) demonstrated significant difference between cyanotic and control group (all P < 0.05).
  
  
  CONCLUSIONS
  Our results extended lung microRNA profile in immature piglets and suggested that miR-370 and miR-320 are significantly up-regulated in cyanotic lung tissues.
authors.affiliation: Department of Pediatric Cardiac Center, Beijing Anzhen Hospital, Capital Medical University, Beijng 100029, China.
authors.forename: Dong
authors.initials: D
authors.lastname: Wang
authors.forename: Ying-Long
authors.initials: YL
authors.lastname: Liu
authors.forename: Xiao-Dong
authors.initials: XD
authors.lastname: Lü
authors.forename: Feng
authors.initials: F
authors.lastname: Ling
authors.forename: Ai-Jun
authors.initials: AJ
authors.lastname: Liu
authors.forename: Jie
authors.initials: J
authors.lastname: DU
authors.forename: Ling
authors.initials: L
authors.lastname: Han
mesh_terms: D000818:Animals; D002908:Chronic Disease; D003490:Cyanosis / Q000235:genetics* / Q000503:physiopathology; D020869:Gene Expression Profiling; D006325:Heart Atria / Q000601:surgery; D035683:MicroRNAs / Q000032:analysis / Q000502:physiology*; D011651:Pulmonary Artery / Q000601:surgery; D011652:Pulmonary Circulation; D060888:Real-Time Polymerase Chain Reaction; D013552:Swine; D013556:Swine, Miniature
chemical_list: D035683:MicroRNAs