title: Wide field-of-view Talbot grid-based microscopy for multicolor fluorescence imaging.
abstract: The capability to perform multicolor, wide field-of-view (FOV) fluorescence microscopy imaging is important in screening and pathology applications. We developed a microscopic slide-imaging system that can achieve multicolor, wide FOV, fluorescence imaging based on the Talbot effect. In this system, a light-spot grid generated by the Talbot effect illuminates the sample. By tilting the excitation beam, the Talbot-focused spot scans across the sample. The images are reconstructed by collecting the fluorescence emissions that correspond to each focused spot with a relay optics arrangement. The prototype system achieved an FOV of 12 × 10 mm(2) at an acquisition time as fast as 23 s for one fluorescence channel. The resolution is fundamentally limited by spot size, with a demonstrated full-width at half-maximum spot diameter of 1.2 μm. The prototype was used to nimage green fluorescent beads, double-stained human breast cancer SK-BR-3 cells, Giardia lamblia cysts, and the Cryptosporidium parvum oocysts. This imaging method is scalable and simple for implementation of high-speed wide FOV fluorescence microscopy.
authors.affiliation: Department of Electrical Engineering, California Institute of Technology, 1200 E. California Blvd. Pasadena, CA 91125, USA. spang@caltech.edu
authors.forename: Shuo
authors.initials: S
authors.lastname: Pang
authors.forename: Chao
authors.initials: C
authors.lastname: Han
authors.forename: Jessey
authors.initials: J
authors.lastname: Erath
authors.forename: Ana
authors.initials: A
authors.lastname: Rodriguez
authors.forename: Changhuei
authors.initials: C
authors.lastname: Yang
mesh_terms: D001943:Breast Neoplasms / Q000473:pathology*; D045744:Cell Line, Tumor; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006801:Humans; D007089:Image Enhancement / Q000295:instrumentation*; D008029:Lighting / Q000295:instrumentation*; D036641:Microscopy, Fluorescence, Multiphoton / Q000295:instrumentation*