title: Accurate assessment of HER2 gene status for invasive component of breast cancer by combination of immunohistochemistry and chromogenic In Situ hybridization.
abstract: The specimens of ductal carcinoma in situ (DCIS) with early invasion, and specimens collected by core needle biopsy (CNB) tend to contain limited amount of invasive component, so it is imperative to explore a new technique which can assess HER2 gene status accurately for the limited invasive cancer component in these specimens. Dual staining technique of combining immunohistochemistry (IHC) for myoepithelial cells and single or dual probe chromogenic in situ hybridization (CISH) for HER2 gene was performed on routinely processed paraffin sections from 20 cases diagnosed as having DCIS with invasive cancer. Among them, 10 had fluorescence in situ hybridization (FISH)-confirmed amplification of HER2 and 10 had FISH-confirmed non-amplification of HER2. We successfully detected HER2 genetic signals and myoepithelial IHC markers (SMM-HC or CK5/6) simultaneously on a single section in all 20 specimens. Myoepithelial markers and HER2 signals detected by dual staining assay were consistent with those by individual technique performed alone. HER2 gene amplification results determined by dual staining assay were 100% consistent with those of FISH. Dual staining technique which allows simultaneous detection of myoepithelial marker protein and cancerous HER2 gene is feasible, and it has potential to be used in clinical practice for effective determination of HER2 amplification in limited invasive component.
authors.affiliation: Department of Pathology, Huazhong University of Science and Technology, Wuhan, 430022, China.
authors.forename: Xiu
authors.initials: X
authors.lastname: Nie
authors.affiliation: Department of Pathology, Huazhong University of Science and Technology, Wuhan, 430022, China.
authors.forename: Jun
authors.initials: J
authors.lastname: He
authors.affiliation: Department of Pathology, Huazhong University of Science and Technology, Wuhan, 430022, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Pathology, Huazhong University of Science and Technology, Wuhan, 430022, China.
authors.forename: Dan-Zhen
authors.initials: DZ
authors.lastname: Pan
authors.affiliation: Department of Pathology, Huazhong University of Science and Technology, Wuhan, 430022, China.
authors.forename: Hua-Xiong
authors.initials: HX
authors.lastname: Pan
authors.affiliation: Department of Pathology, Huazhong University of Science and Technology, Wuhan, 430022, China.
authors.forename: Mi-Xia
authors.initials: MX
authors.lastname: Weng
authors.affiliation: Department of Pathology, Huazhong University of Science and Technology, Wuhan, 430022, China.
authors.forename: Xiu-Ping
authors.initials: XP
authors.lastname: Yang
authors.affiliation: Department of Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China.
authors.forename: Chun-Ping
authors.initials: CP
authors.lastname: Liu
authors.affiliation: Department of Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China. huangtaowh@163.com.
authors.forename: Tao
authors.initials: T
authors.lastname: Huang
mesh_terms: D014408:Biomarkers, Tumor / Q000378:metabolism*; D001943:Breast Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D002863:Chromogenic Compounds; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods*; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D009361:Neoplasm Invasiveness / Q000473:pathology / Q000503:physiopathology; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000235:genetics / Q000378:metabolism*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D014408:Biomarkers, Tumor; D002863:Chromogenic Compounds; D018719:Erb-b2 Receptor Tyrosine Kinases; C508053:ERBB2 protein, human