title: Detection of promyelocytic leukemia/retinoic acid receptor α (PML/RARα) fusion gene with functionalized graphene oxide.
abstract: An attempt was made to use functionalized graphene oxide (GO) to detect the Promyelocytic leukemia/Retinoic acid receptor α fusion gene (PML/RARα fusion gene), a marker gene of acute promyelocytic leukemia. The functionalized GO was prepared by chemical exfoliation method, followed by a polyethylene glycol grafting. It is found that the functionalized GO can selectively adsorb the fluorescein isothiocyanate (FITC)-labeled single-stranded DNA probe and quench its fluorescence. The probe can be displaced by the PML/RARα fusion gene to restore the fluorescence, which can be detected by laser confocal microscopy and flow cytometry. These can be used to detect the presence of the PML/RARα fusion gene. This detection method is verified to be fast, simple and reliable.
authors.affiliation: Department of Hematology, the Second Hospital of Shanxi Medical University, Taiyuan 030001, China. wanghw68@hotmail.com.
authors.forename: Ran
authors.initials: R
authors.lastname: Li
authors.forename: Yanhong
authors.initials: Y
authors.lastname: Tan
authors.forename: Xiuhua
authors.initials: X
authors.lastname: Chen
authors.forename: Fanggang
authors.initials: F
authors.lastname: Ren
authors.forename: Yaofang
authors.initials: Y
authors.lastname: Zhang
authors.forename: Zhifang
authors.initials: Z
authors.lastname: Xu
authors.forename: Hongwei
authors.initials: H
authors.lastname: Wang
mesh_terms: D000327:Adsorption; D045744:Cell Line, Tumor; D004247:DNA / Q000378:metabolism; D005434:Flow Cytometry; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D018613:Microscopy, Confocal; D015514:Oncogene Proteins, Fusion / Q000235:genetics*; D010087:Oxides / Q000737:chemistry*; D012996:Solutions; D013050:Spectrometry, Fluorescence
chemical_list: D015514:Oncogene Proteins, Fusion; D010087:Oxides; D012996:Solutions; C093476:promyelocytic leukemia-retinoic acid receptor alpha fusion oncoprotein; D006108:Graphite; D004247:DNA