title: Oral immunization of mice with Saccharomyces cerevisiae expressing a neutralizing epitope of ApxIIA exotoxin from Actinobacillus pleuropneumoniae induces systemic and mucosal immune responses.
abstract: An oral delivery system based on ApxIIA#5-expressed on Saccharomyces cerevisiae was studied for its potential to induce immune responses in mice. Murine bone marrow-derived dendritic cells (DCs) stimulated in vitro with ApxIIA#5-expressed on S. cerevisiae upregulated the expression of maturation and activation markers, leading to production of tumor necrosis factor-α, interleukin (IL)-1β, IL-12p70 and IL-10. Presentation of these activated DCs to cluster of differentiation CD4+ T cells collected from mice that had been orally immunized with the ApxIIA#5-expressed on S. cerevisiae elicited specific T-cell proliferation. In addition, the orally immunized mice had stronger antigen-specific serum IgG and IgA antibody responses and larger numbers of antigen-specific IgG and IgA antibody-secreting cells in their spleens, Peyer's patches and lamina propria than did those immunized with vector-only S. cerevisiae or those not immunized. Furthermore, oral immunization induced T helper 1-type immune responses mediated via increased serum concentrations of IgG2a and an increase predominantly of IFN-γ-producing cells in their spleens and lamina propria. Our findings suggest that surface-displayed ApxIIA#5-expressed on S. cerevisiae may be a promising candidate for an oral vaccine delivery system for eliciting systemic and mucosal immunity.
authors.affiliation: Department of Infectious Disease, College of Veterinary Medicine, Brain Korea 21 Program for Veterinary Science, Seoul National University, Gwanak 599 Gwanak-ro, Gwanak-gu, Seoul 151-742, Korea.
authors.forename: Min-Kyoung
authors.initials: MK
authors.lastname: Shin
authors.forename: Won-Jung
authors.initials: WJ
authors.lastname: Lee
authors.forename: Myung Hwan
authors.initials: MH
authors.lastname: Jung
authors.forename: Seung-Bin
authors.initials: SB
authors.lastname: Cha
authors.forename: Seung-Won
authors.initials: SW
authors.lastname: Shin
authors.forename: Anna
authors.initials: A
authors.lastname: Yoo
authors.forename: Dae-Hyuk
authors.initials: DH
authors.lastname: Kim
authors.forename: Han Sang
authors.initials: HS
authors.lastname: Yoo
mesh_terms: D000189:Actinobacillus Infections / Q000276:immunology / Q000517:prevention & control*; D016977:Actinobacillus pleuropneumoniae / Q000235:genetics / Q000276:immunology*; D000284:Administration, Oral; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D001426:Bacterial Proteins / Q000235:genetics / Q000276:immunology*; D001428:Bacterial Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D049109:Cell Proliferation; D016207:Cytokines / Q000378:metabolism; D003713:Dendritic Cells / Q000276:immunology; D006460:Hemolysin Proteins / Q000235:genetics / Q000276:immunology*; D018928:Immunity, Mucosal; D007070:Immunoglobulin A / Q000097:blood; D007074:Immunoglobulin G / Q000097:blood; D051379:Mice; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000276:immunology*
chemical_list: D000907:Antibodies, Bacterial; C085361:ApxII toxin, bacteria; D001426:Bacterial Proteins; D001428:Bacterial Vaccines; D016207:Cytokines; D006460:Hemolysin Proteins; D007070:Immunoglobulin A; D007074:Immunoglobulin G