title: Isolation, culture and evaluation of multilineage-differentiating stress-enduring (Muse) cells.
abstract: Multilineage-differentiating stress-enduring (Muse) cells are distinct stem cells in mesenchymal cell populations with the capacity to self-renew, to differentiate into cells representative of all three germ layers from a single cell, and to repair damaged tissues by spontaneous differentiation into tissue-specific cells without forming teratomas. We describe step-by-step procedures for isolating and evaluating these cells. Muse cells are also a practical cell source for human induced pluripotent stem (iPS) cells with markedly high generation efficiency. They can be collected as cells that are double positive for stage-specific embryonic antigen-3 (SSEA-3) and CD105 from commercially available mesenchymal cells, such as adult human bone marrow stromal cells and dermal fibroblasts, or from fresh adult human bone marrow samples. Under both spontaneous and induced differentiation conditions, they show triploblastic differentiation. It takes 4-6 h to collect and 2 weeks to confirm the differentiation and self-renewal capacity of Muse cells.
authors.affiliation: Department of Anatomy and Anthropology, Tohoku University Graduate School of Medicine, Sendai, Japan. y-kuroda@med.tohoku.ac.jp
authors.forename: Yasumasa
authors.initials: Y
authors.lastname: Kuroda
authors.forename: Shohei
authors.initials: S
authors.lastname: Wakao
authors.forename: Masaaki
authors.initials: M
authors.lastname: Kitada
authors.forename: Toru
authors.initials: T
authors.lastname: Murakami
authors.forename: Makoto
authors.initials: M
authors.lastname: Nojima
authors.forename: Mari
authors.initials: M
authors.lastname: Dezawa
mesh_terms: D015703:Antigens, CD / Q000378:metabolism; D015295:Antigens, Tumor-Associated, Carbohydrate / Q000378:metabolism; D001854:Bone Marrow Cells / Q000166:cytology; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation; D019070:Cell Lineage / Q000502:physiology*; D002478:Cells, Cultured; D000071063:Endoglin; D005434:Flow Cytometry / Q000379:methods; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology / Q000378:metabolism; D008648:Mesoderm / Q000166:cytology; D011956:Receptors, Cell Surface / Q000378:metabolism; D055746:Stage-Specific Embryonic Antigens / Q000378:metabolism; D013234:Stem Cells / Q000166:cytology*
chemical_list: D015703:Antigens, CD; D015295:Antigens, Tumor-Associated, Carbohydrate; C064206:ENG protein, human; D000071063:Endoglin; D011956:Receptors, Cell Surface; D055746:Stage-Specific Embryonic Antigens; C052924:stage-specific embryonic antigen-3