title: Tropomyosin contains IgE-binding epitopes sensitive to periodate but not to enzymatic deglycosylation.
abstract: Glycosylation has been reported to affect the epitopes of food allergens, however, there are few reports on its role in crab allergen. In the present study, the effect of glycosylation on the IgE-binding activity of tropomyosin (TM), a major allergen in Scylla paramamosain, was investigated. The results showed that TM was a glycoprotein with a 0.2% carbohydrate moiety and contained O-glycan. Moreover, enzymatic deglycosylation of TM by glycosidase had no effect on the IgE-binding activity of TM. In contrast, treatment with periodate resulted in a significant reduction in its IgE-binding activity.
authors.affiliation: College of Biological Engineering, The Key Laboratory of Science and Technology for Aquaculture and Food Safety, Jimei Univ., 43 Yindou Rd., Xiamen, Fujian 361021, China.
authors.forename: Wei-Wei
authors.initials: WW
authors.lastname: Ruan
authors.forename: Min-Jie
authors.initials: MJ
authors.lastname: Cao
authors.forename: Feng
authors.initials: F
authors.lastname: Chen
authors.forename: Qiu-Feng
authors.initials: QF
authors.lastname: Cai
authors.forename: Wen-Jin
authors.initials: WJ
authors.lastname: Su
authors.forename: You-Zhao
authors.initials: YZ
authors.lastname: Wang
authors.forename: Guang-Ming
authors.initials: GM
authors.lastname: Liu
mesh_terms: D000485:Allergens / Q000276:immunology / Q000378:metabolism; D000818:Animals; D003386:Brachyura / Q000276:immunology; D000939:Epitopes / Q000737:chemistry* / Q000276:immunology; D005512:Food Hypersensitivity / Q000276:immunology*; D006031:Glycosylation; D007073:Immunoglobulin E / Q000737:chemistry* / Q000276:immunology; D043524:Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase / Q000378:metabolism; D010504:Periodic Acid / Q000378:metabolism*; D011134:Polysaccharides / Q000737:chemistry / Q000276:immunology; D011485:Protein Binding; D014335:Tropomyosin / Q000737:chemistry* / Q000276:immunology
chemical_list: D000485:Allergens; D000939:Epitopes; D011134:Polysaccharides; D014335:Tropomyosin; D010504:Periodic Acid; D007073:Immunoglobulin E; C009288:metaperiodate; D043524:Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase
keywords: IgE-binding activity; deglycosylation; glycoprotein; periodate treatment; tropomyosin