title: Morphological, functional and biochemical characterization of canine gingival fibroblasts.
abstract: As dogs are good models for in vivo studies, it is interesting to evaluate the behavior of canine gingival fibroblasts (CGF) in vitro, so that these cells could be seeded on a matrix and later studied in vivo. The aim of this study was to perform a morphological, functional and biochemical analysis of CGF, comparing it with human gingival fibroblasts (HGF), as well as to evaluate the change of their characteristics over several passages. Using gingival fibroblasts from 3 dogs and 3 humans in the subculture (Sub), first (P1), third (P3), fifth (P5) and seventh (P7) passages, the following parameters were assessed: cell morphology, spreading, adhesion, viability and total protein content. The results showed no major differences between the passages in terms of morphology and spreading, and a tendency of greater adhesion and viability for HGF when compared with CGF. The total protein content was significantly higher for HGF. HGF exhibited greater functional and biochemical activity in vitro compared to CGF. Higher numbers at Sub were observed for both CGF and HGF in all evaluated parameters. The differences do not prevent the use of CGF for tissue engineering, but its use seems to be more appropriate in the subculture or first passage.
authors.affiliation: Department of Traumatology and Bucomaxillofacial Surgery and Periodontology, Ribeirão Preto Dental School, USP - University of São Paulo, Ribeirão Preto, SP, Brazil.
authors.forename: Camila Bonvicino
authors.initials: CB
authors.lastname: Pelegrini
authors.forename: Luciana Prado
authors.initials: LP
authors.lastname: Maia
authors.forename: Sérgio Luís Scombatti
authors.initials: SL
authors.lastname: de Souza
authors.forename: Mário
authors.initials: M
authors.lastname: Taba
authors.forename: Daniela Bazan
authors.initials: DB
authors.lastname: Palioto
mesh_terms: D000818:Animals; D002448:Cell Adhesion / Q000502:physiology; D002452:Cell Count; D018929:Cell Culture Techniques; D002465:Cell Movement / Q000502:physiology; D048430:Cell Shape; D002470:Cell Survival / Q000502:physiology; D002478:Cells, Cultured; D004285:Dogs; D005347:Fibroblasts / Q000166:cytology* / Q000378:metabolism / Q000502:physiology; D005881:Gingiva / Q000166:cytology*; D006801:Humans; D023421:Models, Animal; D061251:Primary Cell Culture; D011506:Proteins / Q000032:analysis; D013997:Time Factors; D023822:Tissue Engineering / Q000379:methods
chemical_list: D011506:Proteins