title: Ultra-high-performance liquid chromatography-ion trap mass spectrometry characterisation of milk polar lipids from dairy cows fed different diets.
abstract: Milk polar lipids are an important class of biologically active species for human health and for improving the physical functionality of food ingredients. Milk polar lipids from 144 multiparous Holstein-Friesian dairy cows fed different diets were analysed using ultra-high-performance liquid chromatography-ion trap mass spectrometry (UHPLC-MS(n)). A complex profile of polar lipids, consisting of 7 species of phosphatidylinositol (PI), 12 species of phosphatidylethanolamine (PE), 18 species of phosphatidylcholine (PC) and 13 species of sphingomyelin (SM) were identified from the molecular ions and sequential MS(n) fragmentation. Qualitative assessment of the data suggested that different cow diets influenced the relative amounts of a small number of species in the milk samples, e.g. PE 14:0/18:1, PE 18:0/18:1, PC 15:0/18:1, PC 18:0/18:1, SM d18:1/14:0, SM d18:1/15:0, SM d18:1/22:0 and SM d18:1/23:0.
authors.affiliation: Department of Primary Industries, Knoxfield Centre, 621 Burwood Highway, Knoxfield, Victoria 3180, Australia.
authors.forename: V
authors.initials: V
authors.lastname: Craige Trenerry
authors.forename: Ghazal
authors.initials: G
authors.lastname: Akbaridoust
authors.forename: Tim
authors.initials: T
authors.lastname: Plozza
authors.forename: Simone
authors.initials: S
authors.lastname: Rochfort
authors.forename: William J
authors.initials: WJ
authors.lastname: Wales
authors.forename: Martin
authors.initials: M
authors.lastname: Auldist
authors.forename: Said
authors.initials: S
authors.lastname: Ajlouni
mesh_terms: D000821:Animal Feed / Q000032:analysis*; D000818:Animals; D002417:Cattle / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005260:Female; D050356:Lipid Metabolism; D008055:Lipids / Q000737:chemistry*; D013058:Mass Spectrometry / Q000379:methods; D008892:Milk / Q000737:chemistry* / Q000378:metabolism; D015394:Molecular Structure
chemical_list: D008055:Lipids