title: Indirect competitive ELISA based on monoclonal antibody for the detection of 5-hydroxymethyl-2-furfural in milk, compared with HPLC.
abstract: In this study, a method for rapid detection of 5-hydroxymethyl-2-furfural (HMF) was investigated. Monoclonal antibody (anti-HMF) was prepared and evaluated by an indirect competitive ELISA (ic-ELISA) format. The optimized standard curve was y=-0.2097x+1.0432 [where x is the logarithm (base 10) of the values of the HMF concentration and y is the absorbance of ic-ELISA results tested at 490 nm] and the linear detection range was 0.008 to 32.768 mg/L. The percentage of cross-reactivity of HMF with 5 major furfural derivatives was less than 2.92%. Finally, the established ic-ELISA format was used to test HMF in milk, and compared with the result obtained by HPLC, which produced an error of about 0.3%. Based on the data in this experiment, we concluded that the established ic-ELISA format was reliable with a high specificity.
authors.affiliation: College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, China.
authors.forename: Yongguang
authors.initials: Y
authors.lastname: Guan
authors.forename: Xinlan
authors.initials: X
authors.lastname: Wu
authors.forename: Hecheng
authors.initials: H
authors.lastname: Meng
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005260:Female; D005662:Furaldehyde / Q000031:analogs & derivatives* / Q000032:analysis; D006241:Haptens; D051379:Mice / Q000276:immunology; D008807:Mice, Inbred BALB C / Q000276:immunology; D008892:Milk / Q000737:chemistry*
chemical_list: D000911:Antibodies, Monoclonal; D006241:Haptens; C008046:5-hydroxymethylfurfural; D005662:Furaldehyde
keywords: 5-hydroxymethyl-2-furfural; HPLC; indirect competitive ELISA; monoclonal antibody