title: AdvISER-PYRO: Amplicon Identification using SparsE Representation of PYROsequencing signal.
abstract: BACKGROUND
  Converting a pyrosequencing signal into a nucleotide sequence appears highly challenging when signal intensities are low (unitary peak heights ) or when complex signals are produced by several target amplicons. In these cases, the pyrosequencing software fails to provide correct nucleotide sequences. Accordingly, the objective was to develop the AdvISER-PYRO algorithm, performing an automated, fast and reliable analysis of pyrosequencing signals that circumvents those limitations.
  
  
  RESULTS
  In the current mycobacterial amplicon genotyping application, AdvISER-PYRO performed much better than the pyrosequencing software in the following two situations: when converting Single Amplicon Sample (SAS) signals into a correct single sequence (97.2% versus 56.5%), and when translating Multiple Amplicon Sample (MAS) signals into the correct sequence pair (74.5%).
  
  
  BACKGROUND
  AdvISER-PYRO is implemented in an R package (http://sites.uclouvain.be/md-ctma/index.php/softwares) and can be used in broad range of clinical applications including multiplex pyrosequencing and oncogene re-sequencing in heterogeneous tumor cell samples.
authors.affiliation: Center for Applied Molecular Technologies, Institut de Recherche Expérimentale et Clinique, Université catholique de Louvain, Clos Chapelle-aux-Champs 30, Brussels, Belgium. jerome.ambroise@uclouvain.be
authors.forename: Jérôme
authors.initials: J
authors.lastname: Ambroise
authors.forename: Anne-Sophie
authors.initials: AS
authors.lastname: Piette
authors.forename: Cathy
authors.initials: C
authors.lastname: Delcorps
authors.forename: Leen
authors.initials: L
authors.lastname: Rigouts
authors.forename: Bouke C
authors.initials: BC
authors.lastname: de Jong
authors.forename: Leonid
authors.initials: L
authors.lastname: Irenge
authors.forename: Annie
authors.initials: A
authors.lastname: Robert
authors.forename: Jean-Luc
authors.initials: JL
authors.lastname: Gala
mesh_terms: D000465:Algorithms; D060005:Genotyping Techniques; D009161:Mycobacterium / Q000235:genetics; D017422:Sequence Analysis, DNA / Q000379:methods*; D012984:Software