title: Quick and reversible photocrosslinking reaction of 3-cyanovinylcarbazole nucleoside in a DNA triplex.
abstract: 3-Cyanovinylcarbazole nucleoside ((CNV)K), which can quickly photocrosslink to the pyrimidine base in the DNA double strand, was incorporated into a triplex forming oligonucleotide (TFO). We successfully demonstrated that (CNV)K in TFO can photocrosslink to the thymine base in Watson-Crick double-stranded DNA via anti-Hoogsteen base pairing with a few seconds of photoirradiation.
authors.affiliation: School of Materials Science, Japan Advanced Institute of Science and Technology, 1-1 Asahi-dai, Nomi, Ishikawa 923-1292, Japan. kenzo@jaist.ac.jp
authors.forename: Kenzo
authors.initials: K
authors.lastname: Fujimoto
authors.forename: Hiroki
authors.initials: H
authors.lastname: Yoshinaga
authors.forename: Yasumasa
authors.initials: Y
authors.lastname: Yoshio
authors.forename: Takashi
authors.initials: T
authors.lastname: Sakamoto
mesh_terms: D002227:Carbazoles / Q000737:chemistry*; D003432:Cross-Linking Reagents / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel; D008027:Light; D015394:Molecular Structure; D009705:Nucleosides / Q000737:chemistry*; D013997:Time Factors; D014753:Vinyl Compounds / Q000737:chemistry*
chemical_list: D002227:Carbazoles; D003432:Cross-Linking Reagents; D009705:Nucleosides; D014753:Vinyl Compounds; C051802:triplex DNA; D004247:DNA