title: Effect of lead sulfide nanoparticles exposure on calcium homeostasis in rat hippocampus neurons.
abstract: PbS nanoparticles (NPs) is an important nanomaterial for biomedical imaging in living tissues. However, concerning the high toxicity, especially neurotoxicity, of Pb element, it is crucial that the toxicity assessment of "naked" PbS NPs should be adequately studied. In the current study, we systematically explored the neurotoxicity of PbS NPs in rats by measuring the body weight and brain coefficient changes, testing memory behaviors in Y-electric maze, and studying the neuronal ultrastructure and pathology in hippocampus. Furthermore, in order to study the toxic mechanism, we performed Pb and Ca content measurements in various organs, and investigated Ca(2+)-ATPase activity and L-type calcium channel subunit expression. Our results confirmed that PbS NPs showed high neurotoxicity, while a possible mechanism was suggested to be due to the PbS NPs-induced calcium homeostasis disorder which was caused by the abnormal calcium transportation.
authors.affiliation: Department of Toxicology, School of Public Health, China Medical University, Shenyang 110001, China.
authors.forename: Yanhua
authors.initials: Y
authors.lastname: Cao
authors.forename: Huajie
authors.initials: H
authors.lastname: Liu
authors.forename: Qingzhao
authors.initials: Q
authors.lastname: Li
authors.forename: Qian
authors.initials: Q
authors.lastname: Wang
authors.forename: Wenli
authors.initials: W
authors.lastname: Zhang
authors.forename: Yinping
authors.initials: Y
authors.lastname: Chen
authors.forename: Dong
authors.initials: D
authors.lastname: Wang
authors.forename: Yuan
authors.initials: Y
authors.lastname: Cai
mesh_terms: D000280:Administration, Inhalation; D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects; D001835:Body Weight / Q000187:drug effects; D002118:Calcium / Q000378:metabolism*; D020746:Calcium Channels, L-Type / Q000235:genetics / Q000378:metabolism; D000252:Calcium-Transporting ATPases / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression / Q000187:drug effects; D006624:Hippocampus / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D006706:Homeostasis / Q000187:drug effects*; D017136:Ion Transport / Q000187:drug effects; D007854:Lead / Q000633:toxicity*; D020263:Lead Poisoning, Nervous System / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology; D008297:Male; D018782:Maze Learning / Q000187:drug effects; D053758:Nanoparticles / Q000633:toxicity*; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D021122:Protein Subunits / Q000819:agonists / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013440:Sulfides / Q000633:toxicity*
chemical_list: D020746:Calcium Channels, L-Type; D021122:Protein Subunits; D013440:Sulfides; C018391:lead sulfide; D007854:Lead; D000252:Calcium-Transporting ATPases; D002118:Calcium
keywords: Calcium homeostasis; Hippocampus; Neurotoxicity; PbS nanoparticles