title: Isolation and genetic characterization of an improved benzene-tolerant mutant of Pseudomonas putida S12.
abstract: Pseudomonas putida S12.49, a mutant stain of P. putida S12 that tolerates up to 20 mM benzene, was obtained by evolutionary selection. The genetic basis for the strongly enhanced benzene tolerance was investigated by proteome and transcriptome analysis. Indications were found that the highly benzene-tolerant phenotype is the resultant of multi-level systemic changes. The solvent extrusion pump SrpABC was constitutively expressed in P. putida S12.49, which could be attributed to the disruption of the srpS regulator gene by the indigenous mutator element ISS12. The occurrence of this and two additional transposition events was in good agreement with the increased transcriptional activity of transposase-encoding genes in strain S12.49. These observations suggested that transposition events are an important force driving the generation of the genetic diversity apparently required to obtain highly solvent-tolerant phenotypes. In addition, various expression responses relating to energy generation indicated system changes that accommodated the energy demand associated with the high-level expression of the proton-driven solvent extrusion pump. The relatively modest effect of a respiratory chain uncoupler on benzene tolerance in P. putida S12.49 indicated the involvement of an alternative, non-respiratory mechanism to maintain the proton gradient.
authors.affiliation: TNO Quality of Life, P.O. Box 5054, 2600 GA Delft, the Netherlands. Kluyver Centre for Genomics of Industrial Fermentation, P.O. Box 5057, 2600 GA Delft, the Netherlands. Delft University of Technology, P.O. Box 5045, 2600 GA Delft, the Netherlands.
authors.forename: Rita J M
authors.initials: RJ
authors.lastname: Volkers
authors.forename: Hendrik
authors.initials: H
authors.lastname: Ballerstedt
authors.forename: Johannes H
authors.initials: JH
authors.lastname: De Winde
authors.forename: Harald J
authors.initials: HJ
authors.lastname: Ruijssenaars