title: The components of the angiotensin-(1-7) system are differentially expressed during follicular wave in cattle.
abstract: BACKGROUND
  This study was based on the hypothesis that some components of the angiotensin-(1-7) (Ang-(1-7)) system are differentially expressed during follicular development and can be involved in the follicular health/atresia transition in bovine.
  
  
  METHODS
  The largest (F1) and second largest follicles (F2) were collected from cows before (Day 2), during (Day 3), or after (Day 4) the expected moment of follicular deviation. In the second experiment, F1 was induced to atresia through intrafollicular injection of fulvestrant (estrogen receptor-antagonist) and, in both experiments, mRNA expression of the Mas receptor, ACE2, NEP, and PEP was evaluated in the granulosa and theca cells.
  
  
  RESULTS
  The mRNA expression of Mas receptor was upregulated in the granulosa cells of F2 after the establishment of follicular deviation, while PEP mRNA increased during and after the deviation process. The mRNA expression of ACE2 was upregulated in the granulosa cells of F1 during and after the follicular deviation. The mRNA expression of NEP was not regulated in F1 and F2. Mas receptor expression increased in the F1 induced to atresia.
  
  
  CONCLUSIONS
  mRNA for Mas receptor, ACE2, and PEP are differentially expressed in granulosa cells throughout follicular development and the Mas receptor can be involved with the establishment of follicular dominance.
authors.affiliation: Laboratório de Biotecnologia e Reprodução Animal-BioRep, Universidade Federal de Santa Maria, Brazil Universidade Federal de Santa Catarina, Campus Universitário de Curitibanos, Brazil marcos.barreta@ufsc.br.
authors.forename: Marcos Henrique
authors.initials: MH
authors.lastname: Barreta
authors.affiliation: Laboratório de Biotecnologia e Reprodução Animal-BioRep, Universidade Federal de Santa Maria, Brazil.
authors.forename: Bernardo Garziera
authors.initials: BG
authors.lastname: Gasperin
authors.affiliation: Centro de Educação Superior do Oeste-Universidade do Estado de Santa Catarina, Brasil.
authors.forename: Rogério
authors.initials: R
authors.lastname: Ferreira
authors.affiliation: Laboratório de Biotecnologia e Reprodução Animal-BioRep, Universidade Federal de Santa Maria, Brazil.
authors.forename: Monique
authors.initials: M
authors.lastname: Rovani
authors.affiliation: Laboratório de Biotecnologia e Reprodução Animal-BioRep, Universidade Federal de Santa Maria, Brazil.
authors.forename: Gabriel Ribas
authors.initials: GR
authors.lastname: Pereira
authors.affiliation: Laboratório de Biotecnologia e Reprodução Animal-BioRep, Universidade Federal de Santa Maria, Brazil Department of Animal Science, McGill University, Ste-Anne-De-Bellevue, QC, Canada.
authors.forename: Rodrigo C
authors.initials: RC
authors.lastname: Bohrer
authors.affiliation: Laboratório de Biotecnologia e Reprodução Animal-BioRep, Universidade Federal de Santa Maria, Brazil.
authors.forename: João Francisco
authors.initials: JF
authors.lastname: de Oliveira
authors.affiliation: Laboratório de Biotecnologia e Reprodução Animal-BioRep, Universidade Federal de Santa Maria, Brazil.
authors.forename: Paulo Bayard Dias
authors.initials: PB
authors.lastname: Gonçalves
mesh_terms: D000803:Angiotensin I / Q000235:genetics / Q000378:metabolism*; D000085962:Angiotensin-Converting Enzyme 2; D000818:Animals; D002417:Cattle; D004958:Estradiol / Q000031:analogs & derivatives / Q000494:pharmacology; D005260:Female; D005496:Follicular Atresia / Q000187:drug effects / Q000235:genetics; D000077267:Fulvestrant; D020869:Gene Expression Profiling; D006107:Granulosa Cells / Q000187:drug effects / Q000378:metabolism; D015260:Neprilysin / Q000235:genetics / Q000378:metabolism; D006080:Ovarian Follicle / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D010446:Peptide Fragments / Q000235:genetics / Q000378:metabolism*; D007703:Peptidyl-Dipeptidase A / Q000235:genetics / Q000378:metabolism; D000085822:Prolyl Oligopeptidases; D000090063:Proto-Oncogene Mas; D011518:Proto-Oncogene Proteins / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D043562:Receptors, G-Protein-Coupled / Q000235:genetics / Q000378:metabolism; D012697:Serine Endopeptidases / Q000235:genetics / Q000378:metabolism; D013799:Theca Cells / Q000187:drug effects / Q000378:metabolism
chemical_list: D010446:Peptide Fragments; D000090063:Proto-Oncogene Mas; D011518:Proto-Oncogene Proteins; D012333:RNA, Messenger; D043562:Receptors, G-Protein-Coupled; D000077267:Fulvestrant; D004958:Estradiol; D000803:Angiotensin I; D007703:Peptidyl-Dipeptidase A; D000085962:Angiotensin-Converting Enzyme 2; D012697:Serine Endopeptidases; D000085822:Prolyl Oligopeptidases; D015260:Neprilysin; C118790:angiotensin I (1-7)
keywords: ACE2; Mas receptor; NEP; PEP; bovine; follicular deviation