title: A fast circadian clock at high temperatures is a conserved feature across Arabidopsis accessions and likely to be important for vegetative yield.
abstract: The circadian clock is an endogenous 24 h oscillator regulating many critical biological processes in plants. One of the key characteristics of the circadian clock is that it is buffered against temperature, maintaining an approximately 24 h rhythm over a broad physiological temperature range. Here, we tested temperature-buffering capacity of the circadian clock across a number of Arabidopsis accessions using several circadian clock reporters: leaf movement, CCA1:LUC and LHY:LUC. We found that leaf movement was the best temperature buffered circadian output. On the other hand, when temperature increases, circadian rhythms of CCA1 and LHY transcription shorten considerably across all accessions, indicating that the clock driving expression of CCA1 and LHY is not perfectly buffered. This feature might be crucial to plants growing in a constantly changing environment, and here, we provide insight into the importance of period shortening to plant growth performance and the benefits of a flexible clock.
authors.affiliation: Institute of Integrative Biology, University of Liverpool, Liverpool, L69 7ZB, UK; School of Biological Sciences, University of Bristol, Bristol, BS8 1UG, UK.
authors.forename: Jelena
authors.initials: J
authors.lastname: Kusakina
authors.forename: Peter D
authors.initials: PD
authors.lastname: Gould
authors.forename: Anthony
authors.initials: A
authors.lastname: Hall
mesh_terms: D000704:Analysis of Variance; D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D057906:Circadian Clocks; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant; D008969:Molecular Sequence Data; D013696:Temperature; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; C114425:CCA1 protein, Arabidopsis; D004268:DNA-Binding Proteins; C113192:LHY protein, Arabidopsis; D014157:Transcription Factors
keywords: Arabidopsis thaliana; natural variation; performance