title: Influence of Cyclooxygenase-2 (COX-2) gene promoter-1195 and allograft inflammatory factor-1 (AIF-1) polymorphisms on allograft outcome in Hispanic kidney transplant recipients.
abstract: Cyclooxygenase-2 (COX-2) alleles have been associated with allograft outcomes in kidney transplant recipients; however, these alleles may be in linkage with other genes. Human allograft inflammatory factor-1 (AIF-1) is a cytoplasmic protein and is produced by macrophages. Its synthesis is regulated by several cytokines, including interferon gamma. We investigated whether polymorphisms of gene encoding COX-2 and AIF-1 were associated with allograft outcomes among Hispanic renal transplant recipients (RTRs). A total of 527 de novo RTRs of Hispanic ethnicity were included in this study transplanted at St. Vincent Medical Center (SVMC) during 2000-2009. Patients were genotyped for the following: COX-2 (-1195C>T rs689466, intron 6 rs2066826) and AlF1 (rs2269475). Analysis of the results showed that COX-2-1195 CC genotype (OR=1.92, CI%=1.00-3.67, p=0.04) were more frequent, but COX-2-1195 CT genotype was less frequent in kidney allograft acute rejection in comparison with control group (OR=0.59, CI%=0.38-0.91, p=0.017). The genetic variant TT/CT of the AIF-1 gene was associated with a lower risk of rejection (OR=0.63, CI%=0.41-0.98, p=0.038). No association of COX-2 (rs2066826) was observed with allograft rejection. We are unable to find statistically significant association between COX-2 and AIF-1 gene polymorphisms and allograft survival. The -1195C>T in the COX-2 promoter and AIF-1 gene polymorphisms could be a potential predictor of allograft rejection in our Hispanic kidney transplant recipients.
authors.affiliation: Mendez National Institute of Transplantation, Los Angeles, CA, United States; Saint Vincent Medical Center, Los Angeles, CA, United States; Western University of Health Sciences, Pomona, CA, United States.
authors.forename: Don
authors.initials: D
authors.lastname: Vu
authors.forename: Eglis
authors.initials: E
authors.lastname: Tellez-Corrales
authors.forename: Tariq
authors.initials: T
authors.lastname: Shah
authors.forename: Ian
authors.initials: I
authors.lastname: Hutchinson
authors.forename: David I
authors.initials: DI
authors.lastname: Min
mesh_terms: D000328:Adult; D000483:Alleles; D064591:Allografts / Q000378:metabolism*; D002135:Calcium-Binding Proteins; D051546:Cyclooxygenase 2 / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006084:Graft Rejection / Q000235:genetics; D006085:Graft Survival / Q000235:genetics; D006630:Hispanic or Latino / Q000235:genetics*; D006801:Humans; D016030:Kidney Transplantation; D008297:Male; D008840:Microfilament Proteins; D008875:Middle Aged; D016017:Odds Ratio; D063868:Patient Outcome Assessment; D020641:Polymorphism, Single Nucleotide; D011379:Prognosis; D011401:Promoter Regions, Genetic
chemical_list: C112986:AIF1 protein, human; D002135:Calcium-Binding Proteins; D004268:DNA-Binding Proteins; D008840:Microfilament Proteins; D051546:Cyclooxygenase 2
keywords: AIF-1; AR; COX-2; Cyclooxygenase-2; SNP; acute rejection; allograft inflammatory factor-1; single nucleotide polymorphism