title: Gene expression profile and enrichment pathways in different stages of bladder cancer.
abstract: Bladder cancer is a highly heterogeneous neoplasm. We examined the gene expression profile in 3 bladder cancer stages (Ta, T1, T2) using expression microarray analysis of 40 bladder tumors. Differentially expressed genes were found by the t-test, with <0.005 as the significance threshold. KEGG pathway-enrichment analysis was used to study the signaling pathways of the genes. We found 36 genes that could be used as molecular markers for predicting the transition from Ta-T1 to T1-T2. Among these, 11 overlapped between Ta-T1 and T1-T2 stages. Six genes were down-regulated at the Ta-T1 stage, but were up-regulated at the T1-T2 stage (ANXA5, ATP6V1B2, CTGF, GEM, IL13RA1, and LCP1); 5 genes were up-regulated at the Ta-T1 stage, but down-regulated at the T1-T2 stage (ACPP, GNL1, RIPK1, RAPGEF3, and ZER1). Another 25 genes changed relative expression levels at the T1-T2 stage. These genes (including COL1A1, COL1A2, FN1, ITGA5, LGALS1, SPP1, VIM, POSTN, and COL18A1) may be involved in bladder cancer progression by affecting extracellular matrix-receptor interaction and focal adhesion. The cytokine-cytokine receptor interaction, neuroactive ligand-receptor interaction, and calcium-signaling pathway were associated with bladder cancer progression at both the Ta-T1 and T1-T2 stages.
authors.affiliation: Department of Urology, Center of Nephrology, The Second Affiliated Hospital, The Third Military Medical University, Chongqing, China.
authors.forename: Z-Q
authors.initials: ZQ
authors.lastname: Fang
authors.forename: W-D
authors.initials: WD
authors.lastname: Zang
authors.forename: R
authors.initials: R
authors.lastname: Chen
authors.forename: B-W
authors.initials: BW
authors.lastname: Ye
authors.forename: X-W
authors.initials: XW
authors.lastname: Wang
authors.forename: S-H
authors.initials: SH
authors.lastname: Yi
authors.forename: W
authors.initials: W
authors.lastname: Chen
authors.forename: F
authors.initials: F
authors.lastname: He
authors.forename: G
authors.initials: G
authors.lastname: Ye
mesh_terms: D015972:Gene Expression Regulation, Neoplastic; D052138:Genes, Neoplasm / Q000235:genetics; D006801:Humans; D009367:Neoplasm Staging; D060066:Protein Interaction Maps / Q000235:genetics; D015398:Signal Transduction / Q000235:genetics*; D059467:Transcriptome; D001749:Urinary Bladder Neoplasms / Q000235:genetics* / Q000473:pathology*