title: Effects of short-term refeeding on the expression of genes involved in lipid metabolism in chicks (Gallus gallus).
abstract: The aim of this study was to analyze the expression patterns of key genes involved in lipid metabolism in response to feeding in chicks. A total of 18 thirteen day-old male chicks were fasted for 12h. The mRNA levels of the genes in the liver and white adipose tissue were analyzed after 0, 2, and 4h of refeeding. The mRNA levels of sterol regulatory element-binding protein (SREBP) 1, liver X receptor α, peroxisome proliferator-activated receptor (PPAR) γ, acetyl-CoA carboxylase α and fatty acid synthase were significantly increased after 2h of refeeding. In contrast, the mRNA levels of PPARα and carnitine palmitoyltransferase 1a were significantly decreased after 2h of refeeding. The mRNA level of acyl-CoA oxidase was significantly decreased after 4h of refeeding. The mRNA levels of cholesterol metabolism-related genes such as SREBP2 and 3-hydroxy-3-methylglutaryl-CoA reductase were significantly increased after 2h of refeeding. In the white adipose tissue, the mRNA level of PPARγ was significantly increased after 2h of refeeding, whereas the mRNA level of adipose triglyceride lipase was significantly decreased after 4h of refeeding. These results demonstrated that expression of lipid metabolism-related genes is regulated by short-term refeeding in chicks.
authors.affiliation: Graduate School of Agricultural Science, Kobe University, Kobe 657-8501, Japan.
authors.forename: Takaoki
authors.initials: T
authors.lastname: Saneyasu
authors.forename: Miho
authors.initials: M
authors.lastname: Shiragaki
authors.forename: Kiyotaka
authors.initials: K
authors.lastname: Kurachi
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kamisoyama
authors.forename: Kazuhisa
authors.initials: K
authors.lastname: Honda
mesh_terms: D000103:Acetyl-CoA Carboxylase / Q000096:biosynthesis; D000818:Animals; D002645:Chickens / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D005215:Fasting / Q000378:metabolism*; D064429:Fatty Acid Synthases / Q000096:biosynthesis; D005247:Feeding Behavior; D005786:Gene Expression Regulation; D050356:Lipid Metabolism / Q000235:genetics*; D000071518:Liver X Receptors; D008297:Male; D057093:Orphan Nuclear Receptors / Q000096:biosynthesis; D047495:PPAR gamma / Q000096:biosynthesis; D012333:RNA, Messenger / Q000096:biosynthesis*; D051780:Sterol Regulatory Element Binding Protein 1 / Q000096:biosynthesis
chemical_list: D000071518:Liver X Receptors; D057093:Orphan Nuclear Receptors; D047495:PPAR gamma; D012333:RNA, Messenger; D051780:Sterol Regulatory Element Binding Protein 1; D064429:Fatty Acid Synthases; D000103:Acetyl-CoA Carboxylase
keywords: 3-hydroxy-3-methyl glutaryl-CoA reductase; ACC; ACO; ATGL; Bird; CPT1a; CYP7A1; Chickens; FAS; HMGR; HSL; LPL; LXR; Liver; NEFA; PPAR γ; RPS17; Refeeding; SREBP; TAG; Transcription factor; WAT; acetyl-CoA carboxylase; acyl-CoA oxidase; adipose triglyceride lipase; carnitine palmitoyltransferase 1a; cholesterol 7α-hydroxylase; fatty acid synthase; hormone sensitive lipase; lipoprotein lipase; liver X receptor; non-esterified fatty acids; peroxisome proliferator-activated receptor; ribosomal protein S17; sterol regulatory element-binding protein; triglyceride; white adipose tissue