title: Accelerating the early angiogenesis of tissue engineering constructs in vivo by the use of stem cells cultured in matrigel.
abstract: In tissue engineering research, generating constructs with an adequate extent of clinical applications remains a major challenge. In this context, rapid blood vessel ingrowth in the transplanted tissue engineering constructs is the key factor for successful incorporation. To accelerate the microvascular development in engineered tissues, we preincubated osteoblast-like cells as well as mesenchymal stem cells or a combination of both cell types in Matrigel-filled PLGA scaffolds before transplantation into the dorsal skinfold chambers of balb/c mice. By the use of preincubated mesenchymal stem cells, a significantly accelerated angiogenesis was achieved. Compared with previous studies that showed a decisive increase of vascularization on day 6 after the implantation, we were able to halve this period and achieve explicitly denser microvascular networks 3 days after transplantation of the tissue engineering constructs. Thereby, the inflammatory host tissue response was acceptable and low, comparable with former investigations. A co-incubation of osteoblast-like cells and stem cells showed no additive effect on the density of the newly formed microvascular network. Preincubation of mesenchymal stem cells in Matrigel is a promising approach to develop rapid microvascular growth into tissue engineering constructs. After the implantation into the host organism, scaffolds comprising stem cells generate microvascular capillary-like structures exceptionally fast. Thereby, transplanted stem cells likely differentiate into vessel-associated cells. For this reason, preincubation of mesenchymal stem cells in nutrient solutions supporting different steps of angiogenesis provides a technique to promote the routine use of tissue engineering in the clinic.
authors.affiliation: Department of Oral and Maxillofacial Surgery, Hannover Medical School, Hannover, Germany.
authors.forename: Paul
authors.initials: P
authors.lastname: Schumann
authors.forename: Daniel
authors.initials: D
authors.lastname: Lindhorst
authors.forename: Constantin
authors.initials: C
authors.lastname: von See
authors.forename: Nadine
authors.initials: N
authors.lastname: Menzel
authors.forename: Andreas
authors.initials: A
authors.lastname: Kampmann
authors.forename: Frank
authors.initials: F
authors.lastname: Tavassol
authors.forename: Horst
authors.initials: H
authors.lastname: Kokemüller
authors.forename: Majeed
authors.initials: M
authors.lastname: Rana
authors.forename: Nils-Claudius
authors.initials: NC
authors.lastname: Gellrich
authors.forename: Martin
authors.initials: M
authors.lastname: Rücker
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D003094:Collagen / Q000737:chemistry*; D004338:Drug Combinations; D006439:Hemodynamics; D019344:Lactic Acid / Q000737:chemistry*; D007797:Laminin / Q000737:chemistry*; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D051379:Mice; D008807:Mice, Inbred BALB C; D055806:Microvessels; D018919:Neovascularization, Physiologic; D010006:Osteoblasts / Q000166:cytology / Q000637:transplantation; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011509:Proteoglycans / Q000737:chemistry*; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*; D042461:Vascular Endothelial Growth Factor A / Q000032:analysis
chemical_list: D004338:Drug Combinations; D007797:Laminin; D011509:Proteoglycans; D042461:Vascular Endothelial Growth Factor A; C059485:matrigel; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D003094:Collagen
keywords: dorsal skinfold chamber; intravital fluorescence microscopy; osteoblast-like cells; vascularization; vitalized scaffold