title: Chlamydia trachomatis recombinant MOMP encapsulated in PLGA nanoparticles triggers primarily T helper 1 cellular and antibody immune responses in mice: a desirable candidate nanovaccine.
abstract: We recently demonstrated by in vitro experiments that PLGA (poly D, L-lactide-co-glycolide) potentiates T helper 1 (Th1) immune responses induced by a peptide derived from the recombinant major outer membrane protein (rMOMP) of Chlamydia trachomatis, and may be a promising vaccine delivery system. Herein we evaluated the immune-potentiating potential of PLGA by encapsulating the full-length rMOMP (PLGA-rMOMP), characterizing it in vitro, and investigating its immunogenicity in vivo. Our hypothesis was that PLGA-rMOMP triggers Th1 immune responses in mice, which are desirable prerequisites for a C. trachomatis candidate nanovaccine. Physical-structural characterizations of PLGA-rMOMP revealed its size (approximately 272 nm), zeta potential (-14.30 mV), apparent spherical smooth morphology, and continuous slow release pattern. PLGA potentiated the ability of encapsulated rMOMP to trigger production of cytokines and chemokines by mouse J774 macrophages. Flow cytometric analyses revealed that spleen cells from BALB/c mice immunized with PLGA-rMOMP had elevated numbers of CD4+ and CD8+ T cell subsets, and secreted more rMOMP-specific interferon-gamma (Th1) and interleukin (IL)-12p40 (Th1/Th17) than IL-4 and IL-10 (Th2) cytokines. PLGA-rMOMP-immunized mice produced higher serum immunoglobulin (Ig)G and IgG2a (Th1) than IgG1 (Th2) rMOMP-specific antibodies. Notably, sera from PLGA-rMOMP-immunized mice had a 64-fold higher Th1 than Th2 antibody titer, whereas mice immunized with rMOMP in Freund's adjuvant had only a four-fold higher Th1 than Th2 antibody titer, suggesting primarily induction of a Th1 antibody response in PLGA-rMOMP-immunized mice. Our data underscore PLGA as an effective delivery system for a C. trachomatis vaccine. The capacity of PLGA-rMOMP to trigger primarily Th1 immune responses in mice promotes it as a highly desirable candidate nanovaccine against C. trachomatis.
authors.affiliation: Center for NanoBiotechnology Research, Alabama State University, Montgomery, AL 36104, USA.
authors.forename: Stacie J
authors.initials: SJ
authors.lastname: Fairley
authors.forename: Shree R
authors.initials: SR
authors.lastname: Singh
authors.forename: Abebayehu N
authors.initials: AN
authors.lastname: Yilma
authors.forename: Alain B
authors.initials: AB
authors.lastname: Waffo
authors.forename: Praseetha
authors.initials: P
authors.lastname: Subbarayan
authors.forename: Saurabh
authors.initials: S
authors.lastname: Dixit
authors.forename: Murtada A
authors.initials: MA
authors.lastname: Taha
authors.forename: Chino D
authors.initials: CD
authors.lastname: Cambridge
authors.forename: Vida A
authors.initials: VA
authors.lastname: Dennis
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D001428:Bacterial Vaccines / Q000737:chemistry / Q000276:immunology* / Q000493:pharmacokinetics; D002460:Cell Line; D018925:Chemokines / Q000032:analysis / Q000378:metabolism; D002692:Chlamydia trachomatis / Q000276:immunology*; D016207:Cytokines / Q000032:analysis / Q000378:metabolism; D005260:Female; D005434:Flow Cytometry; D019344:Lactic Acid / Q000737:chemistry; D008264:Macrophages; D051379:Mice; D008807:Mice, Inbred BALB C; D053758:Nanoparticles / Q000737:chemistry*; D011100:Polyglycolic Acid / Q000737:chemistry; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D018272:Porins / Q000737:chemistry / Q000276:immunology* / Q000493:pharmacokinetics; D011994:Recombinant Proteins / Q000737:chemistry / Q000276:immunology / Q000493:pharmacokinetics; D018417:Th1 Cells; D022223:Vaccines, Subunit / Q000737:chemistry / Q000276:immunology* / Q000493:pharmacokinetics
chemical_list: D000907:Antibodies, Bacterial; D001428:Bacterial Vaccines; D018925:Chemokines; D016207:Cytokines; D018272:Porins; D011994:Recombinant Proteins; D022223:Vaccines, Subunit; C071677:omp1 protein, Chlamydia trachomatis; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid
keywords: Chlamydia trachomatis; PLGA nanoparticles; antibody; bacteria; cytokines; vaccine