title: Effect of resistant starch RS4 added to the high-fat diets on selected biochemical parameters in Wistar rats.
abstract: BACKGROUND
  Resistant starch (RS) is part of potato starch that is not digested in the gastrointestinal tract. RS4 is a chemically modified starch (for example by oxidation and esterification) and physically (by heating).
  
  
  OBJECTIVE
  The study was aimed at determining the effect of resistant starch on lipid metabolism and activity of hepatic enzymes in Wistar strain rats fed high-fat diets containing 15% of lard or 15% of soybean oil.
  
  
  METHODS
  Four types of diets were administered to the animals (4 groups of males, n = 32): control diet (K1) containing 15% of soybean oil; control diet (K2) containing 15% of lard as well as two groups receiving the same diets with 10% addition of resistant starch RS4 (K1S and K2S).
  
  
  RESULTS
  The mean concentration of total cholesterol was lower in the group of animals fed a diet with vegetable oil (39.9 mg/dl) as compared to that reported in the group of rats fed the lard-supplemented diet (55.2 mg/dl). Compared to the control groups in both groups of animals receiving the diet supplemented with resistant starch RS4 the total cholesterol concentration in serum decreased by ca. 25% (differences were statistically significant). In groups of rats receiving oil- or lard-containing diets with the addition of the resistant starch preparation the concentration of triglycerides in serum decreased by ca. 47% and 10%, respectively.
  
  
  CONCLUSIONS
  A beneficial effect of the resistant starch RS4 added to Wistar rats diets on the lipid metabolism has been shown. The concentrations of total cholesterol and triglycerides in the serum were lower and concentration of HDL-cholesterol was higher in the rats fed with the diets containing the addition of the RS4 preparation as compared to the control groups. Based on the activity of hepatic enzymes the degree of liver damage was lower in groups of rats fed with diets containing resistant starch RS4 as compared to the control groups.
authors.affiliation: Faculty of Food Science, Department of Human Nutrition, Wroclaw University of Environmental and Life Sciences, Wroclaw, Poland. monika.bronkowska@wnoz.up.wroc.pl
authors.forename: Monika
authors.initials: M
authors.lastname: Bronkowska
authors.forename: Dagmara
authors.initials: D
authors.lastname: Orzeł
authors.forename: Karolina
authors.initials: K
authors.lastname: Łoźna
authors.forename: Marzena
authors.initials: M
authors.lastname: Styczyńska
authors.forename: Jadwiga
authors.initials: J
authors.lastname: Biernat
authors.forename: Artur
authors.initials: A
authors.lastname: Gryszkin
authors.forename: Tomasz
authors.initials: T
authors.lastname: Zieba
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Kapelko
mesh_terms: D000284:Administration, Oral; D000818:Animals; D002784:Cholesterol / Q000097:blood; D008076:Cholesterol, HDL / Q000097:blood; D059305:Diet, High-Fat; D050356:Lipid Metabolism; D008099:Liver / Q000201:enzymology*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D013213:Starch / Q000008:administration & dosage*; D014280:Triglycerides / Q000097:blood
chemical_list: D008076:Cholesterol, HDL; D014280:Triglycerides; D013213:Starch; D002784:Cholesterol