title: In situ studies on growth, oxidative stress responses, and gene expression of juvenile bighead carp (Hypophthalmichthys nobilis) to eutrophic lake water dominated by cyanobacterial blooms.
abstract: Cyanobacterial blooms have received increasing attention as a public biohazard for human and animal health. To assess the effect of cyanobacteria-dominant lake water on juvenile fish, we measured the responses of specific growth rate, condition factor, body weight and body length, oxidative stress, and related gene expression of juvenile bighead carp Hypophthalmichthys nobilis exposed to in situ eutrophic lake (Chl a was around 7.0μgL(-1)). Results showed in situ cyanobacteria-dominant lake water had no effect on the growth performance, but significantly elevated the contents of malondialdehyde, the expression of heat shock protein 70, and the activity of superoxide dismutase, indicating that oxidative stress occurred. Meanwhile in situ lake water significantly decreased the expression of catalase and glutathione S-transferase genes. We conclude that in situ cyanobacteria-dominated lake water was harmful to juvenile bighead carp based on the oxidative stress and changes in the related gene expression levels.
authors.affiliation: Jiangsu Key Laboratory for Biodiversity and Biotechnology, College of Life Sciences, Nanjing Normal University, 1 Wenyuan Road, Nanjing 210023, China.
authors.forename: Hongjie
authors.initials: H
authors.lastname: Sun
authors.forename: Wenqian
authors.initials: W
authors.lastname: Wang
authors.forename: Linlin
authors.initials: L
authors.lastname: Geng
authors.forename: Yafen
authors.initials: Y
authors.lastname: Chen
authors.forename: Zhou
authors.initials: Z
authors.lastname: Yang
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D002347:Carps / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D000458:Cyanobacteria / Q000502:physiology*; D005068:Eutrophication; D005786:Gene Expression Regulation; D005978:Glutathione / Q000378:metabolism; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism; D060106:Lakes / Q000382:microbiology*; D008099:Liver / Q000378:metabolism; D008315:Malondialdehyde / Q000378:metabolism; D018384:Oxidative Stress; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014871:Water Microbiology
chemical_list: D000975:Antioxidants; D018840:HSP70 Heat-Shock Proteins; D012333:RNA, Messenger; D008315:Malondialdehyde; D005978:Glutathione
keywords: Cyanobacterial blooms; Gene expression; Growth; Hypophthalmichthys nobilis; Oxidative stress