title: Assessing of plasma protein denaturation induced by exposure to cadmium, electromagnetic fields and their combined actions on rat.
abstract: In our environment, we have numerous chances to be exposed to not only electromagnetic fields (EMFs) but also many chemicals containing mutagens. Therefore, the aim of this study was to estimate whether rat's exposure to cadmium and/or EMFs could cause oxidative damage to molecular structure of proteins and whether and to what extent the effects of co-exposure differ from those observed under the treatment with each exposure alone. Thirty-two rats were divided into four groups. Group 1 was termed as control, group 2 was treated with cadmium (3.0 mg/Kg), group 3 was exposed to EMF (10 mT/h/day) and group 4 was treated with cadmium and exposed to EMF. Protein carbonyls (PCO) in the plasma as a marker of oxidative protein damage and total oxidant status (TOS), as well as electrical conductivity and SDS electrophoresis to estimate changes in molecular structure of protein, were determined. The exposure to Cd and/or EMF led to oxidative protein damage (increased PCO and TOS) accomplished by increased stress of electrical charges on the surface of the protein molecule (increased electrical conductivity) and changes in the molecular structure of protein. The effects were more pronounced after treatment with both Cd and EMF than at the treatment with each exposure alone. The serious damage to proteins at the co-exposure to Cd and EMF seems to be due to the interference of the EMF with the toxic activity of cadmium. This work concluded that combined exposure to Cd and EMFs might increase the risk of plasma damage via enhancing free radical generation and protein oxidation.
authors.affiliation: Biophysics Unit, Biochemistry Department, National Research Center , Cairo , Egypt and.
authors.forename: Nahed S
authors.initials: NS
authors.lastname: Hassan
authors.forename: Salwa A
authors.initials: SA
authors.lastname: Abdelkawi
mesh_terms: D000818:Animals; D001798:Blood Proteins / Q000737:chemistry* / Q000378:metabolism; D002104:Cadmium / Q000633:toxicity*; D004574:Electromagnetic Fields / Q000009:adverse effects*; D004785:Environmental Pollutants / Q000633:toxicity*; D008297:Male; D050050:Protein Carbonylation / Q000187:drug effects / Q000528:radiation effects; D011489:Protein Denaturation / Q000187:drug effects* / Q000528:radiation effects*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D001798:Blood Proteins; D004785:Environmental Pollutants; D002104:Cadmium