title: Clinical neurotransplantation protocol for Huntington's and Parkinson's disease.
abstract: OBJECTIVE
  The concept of transplantation of neuronal cells to treat Huntington's and Parkinson's diseases is based on the proven principle that dopaminergic and GABA-ergic progenitor neurons (from the human developing ventral mesencephalon and whole ganglionic eminence) can survive, differentiate and functionally integrate into an allogenic host brain. However, several donor and host-specific variables play a major role in the safety and outcome of this procedure. In this paper, we seek to summarize an updated neural transplantation protocol, based on our institutional experience and many years of collaboration with other neurotransplantation centers.
  
  
  METHODS
  We present a detailed clinical neurotransplantation protocol for Parkinson's (PD) and Huntington's (HD) diseases with special emphasis in understanding the anatomical relationships of the human fetal tissue that are relevant for selection of the desired cell populations.
  
  
  RESULTS
  Two detailed step-wise neurotransplantation protocols are presented, outlining strategies facilitating the avoidance of possible procedure-related complications.
  
  
  CONCLUSIONS
  In this paper we delineated some crucial technical factors enabling the execution of a safe and effective neural transplantation. The protocols presented here might contribute to further development of the experimental clinical neurotransplantation towards a routine therapeutic procedure.
authors.affiliation: Department of Stereotactic and Functional Neurosurgery, University of Freiburg Medical Center, Breisacher, Freiburg, Germany.
authors.forename: William Omar Contreras
authors.initials: WO
authors.lastname: Lopez
authors.forename: Guido
authors.initials: G
authors.lastname: Nikkhah
authors.forename: Ulf D
authors.initials: UD
authors.lastname: Kahlert
authors.forename: Donata
authors.initials: D
authors.lastname: Maciaczyk
authors.forename: Tomasz
authors.initials: T
authors.lastname: Bogiel
authors.forename: Sven
authors.initials: S
authors.lastname: Moellers
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Schültke
authors.forename: Máté
authors.initials: M
authors.lastname: Döbrössy
authors.forename: Jaroslaw
authors.initials: J
authors.lastname: Maciaczyk
mesh_terms: D000818:Animals; D016380:Brain Tissue Transplantation / Q000379:methods* / Q000639:trends; D003342:Corpus Striatum / Q000637:transplantation; D016332:Fetal Tissue Transplantation / Q000379:methods* / Q000639:trends; D006801:Humans; D006816:Huntington Disease / Q000473:pathology / Q000601:surgery*; D008636:Mesencephalon / Q000637:transplantation; D010300:Parkinson Disease / Q000473:pathology / Q000601:surgery*