title: Lack of association between the LRRK2 A419V variant and Asian Parkinson's disease.
abstract: BACKGROUND
  The G2385R and R1628P LRRK2 gene variants have been associated with an increased risk of Parkinson's disease (PD) in the Asian population. Recently, a new LRRK2 gene variant, A419V, was reported to be a third risk variant for PD in Asian patients. Our objective was to investigate this finding in our cohort of Asian subjects.
  
  
  METHODS
  Eight hundred and twenty-eight subjects (404 PD patients, and 424 age and gender-matched control subjects without neurological disorders) were recruited. Genotyping was done by Taqman® allelic discrimination assay on an Applied Biosystems 7500 Fast Real-Time PCR machine.
  
  
  RESULTS
  The heterozygous A419V genotype was found in only 1 patient with PD, compared to 3 in the control group (0.4% vs 1.3%), giving an odds ratio of 0.35 (95% confidence interval (CI), 0.01 to 3.79; P = 0.624).
  
  
  CONCLUSIONS
  A419V is not an important LRRK2 risk variant in our Asian cohort of patients with PD. Our data are further supported by a literature review which showed that 4 out of 6 published studies reported a negative association of this variant in PD.
authors.affiliation: Department of Biomedical Science, Faculty of Medicine, University of Malaya, Malaysia.
authors.forename: Aroma Agape
authors.initials: AA
authors.lastname: Gopalai
authors.forename: Shen Yang
authors.initials: SY
authors.lastname: Lim
authors.forename: Zariah Abdul
authors.initials: ZA
authors.lastname: Aziz
authors.forename: Soo Kun
authors.initials: SK
authors.lastname: Lim
authors.forename: Li Ping
authors.initials: LP
authors.lastname: Tan
authors.forename: Yip Boon
authors.initials: YB
authors.lastname: Chong
authors.forename: Chong Tin
authors.initials: CT
authors.lastname: Tan
authors.forename: Santhi
authors.initials: S
authors.lastname: Puvanarajah
authors.forename: Shanti
authors.initials: S
authors.lastname: Viswanathan
authors.forename: Rishikesan
authors.initials: R
authors.lastname: Kuppusamy
authors.forename: Ai Huey
authors.initials: AH
authors.lastname: Tan
authors.forename: Thien Thien
authors.initials: TT
authors.lastname: Lim
authors.forename: Gaik Bee
authors.initials: GB
authors.lastname: Eow
authors.forename: Mohamed Ibrahim
authors.initials: MI
authors.lastname: Norlinah
authors.forename: Hui Hua
authors.initials: HH
authors.lastname: Li
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhao
authors.forename: Azlina
authors.initials: A
authors.lastname: Ahmad-Annuar
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000409:Alanine / Q000235:genetics*; D016022:Case-Control Studies; D002681:China / Q000208:ethnology; D015331:Cohort Studies; D003596:Cytosine; D005260:Female; D005787:Gene Frequency; D014644:Genetic Variation / Q000235:genetics*; D005838:Genotype; D006579:Heterozygote; D006801:Humans; D007194:India / Q000208:ethnology; D000071158:Leucine-Rich Repeat Serine-Threonine Protein Kinase-2; D008296:Malaysia / Q000208:ethnology; D008297:Male; D008875:Middle Aged; D010300:Parkinson Disease / Q000235:genetics*; D011110:Polymorphism, Genetic / Q000235:genetics; D017346:Protein Serine-Threonine Kinases / Q000235:genetics*; D012307:Risk Factors; D012846:Singapore; D013941:Thymine; D014633:Valine / Q000235:genetics*; D055815:Young Adult
chemical_list: D003596:Cytosine; C495280:LRRK2 protein, human; D000071158:Leucine-Rich Repeat Serine-Threonine Protein Kinase-2; D017346:Protein Serine-Threonine Kinases; D014633:Valine; D000409:Alanine; D013941:Thymine