title: Regeneration of critical bone defects with anionic collagen matrix as scaffolds.
abstract: The aim of this study was to make a histomorphometric evaluation of the osteogenic potential of anionic collagen matrix as scaffolds; either crosslinked in glutaraldehyde or not cross-linked and, implanted in critical bone defects in rat calvaria. Seventy-two rats were randomly distributed in three groups: anionic collagen scaffolds treated for 24 h of selective hydrolysis (ACSH); anionic collagen scaffolds treated for 24 h of selective hydrolysis and 5 min of crosslinking in glutaraldehyde 0.05% (ACSHGA); empty bone defect (Control), evaluated at the biological points of 15, 45, 90 and 120 days. The results showed that the biomaterials implanted were biocompatible and showed a high osteogenic potential. These biomaterials presented a speed of biodegradation compatible with bone neoformation, which was shown to be associated with angiogenesis inside the scaffolds at all biological points. The percentage of mineralization of ACSH (87%) differed statistically from that found in ACSHGA (66%). It was concluded that the regeneration of critical bone defect was more evident in anionic collagen without crosslinking (ACSH).
authors.affiliation: Gonçalo Moniz Research Center, Oswaldo Cruz Foundation, Salvador, Bahia, Brazil, fulviomiguel@yahoo.com.br.
authors.forename: Fúlvio Borges
authors.initials: FB
authors.lastname: Miguel
authors.forename: Aryon de Almeida
authors.initials: Ade A
authors.lastname: Barbosa Júnior
authors.forename: Fabiana Lopes
authors.initials: FL
authors.lastname: de Paula
authors.forename: Isabela Cerqueira
authors.initials: IC
authors.lastname: Barreto
authors.forename: Gilberto
authors.initials: G
authors.lastname: Goissis
authors.forename: Fabiana Paim
authors.initials: FP
authors.lastname: Rosa
mesh_terms: D000818:Animals; D000838:Anions; D001842:Bone and Bones / Q000002:abnormalities*; D003094:Collagen; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012038:Regeneration; D054457:Tissue Scaffolds
chemical_list: D000838:Anions; D003094:Collagen