title: Shiga toxin-producing Escherichia coli in yaks (Bos grunniens) from the Qinghai-Tibetan Plateau, China.
abstract: Shiga toxin (Stx)-producing Escherichia coli (STEC) are recognized as important human pathogens of public health concern. Many animals are the sources of STEC. In this study we determined the occurrence and characteristics of the STEC in yaks (Bos grunniens) from the Qinghai-Tibetan plateau, China. A total of 728 yak fecal samples was collected from June to August, 2012 and was screened for the presence of the stx 1 and stx 2 genes by TaqMan real-time PCR after the sample was enriched in modified Tryptone Soya Broth. Of the 138 (18.96%) stx 1 and/or stx 2-positive samples, 85 (61.59%) were confirmed to have at least 1 STEC isolate present by culture isolation, from which 128 STEC isolates were recovered. All STEC isolates were serotyped, genotyped by pulsed-field gel electrophoresis (PFGE) and characterized for the presence of 16 known virulence factors. Fifteen different O serogroups and 36 different O:H serotypes were identified in the 128 STEC isolates with 21 and 4 untypable for the O and H antigens respectively. One stx 1 subtype (stx 1a) and 5 stx 2 subtypes (stx 2a, stx 2b, stx 2c, stx 2d and stx 2g) were present in these STEC isolates. Apart from lpfA O157/OI-141, lpfA O157/OI-154, lpfA O113, katP and toxB which were all absent, other virulence factors screened (eaeA, iha, efa1, saa, paa, cnf1, cnf2, astA, subA, exhA and espP) were variably present in the 128 STEC isolates. PFGE were successful for all except 5 isolates and separated them into 67 different PFGE patterns. For the 18 serotypes with 2 or more isolates, isolates of the same serotypes had the same or closely related PFGE patterns, demonstrating clonality of these serotypes. This study was the first report on occurrence and characteristics of STEC isolated from yaks (Bos grunniens) from the Qinghai-Tibetan plateau, China, and extended the genetic diversity and reservoir host range of STEC.
authors.affiliation: Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Changping, Beijing, China.
authors.forename: Xiangning
authors.initials: X
authors.lastname: Bai
authors.forename: Ailan
authors.initials: A
authors.lastname: Zhao
authors.forename: Ruiting
authors.initials: R
authors.lastname: Lan
authors.forename: Youquan
authors.initials: Y
authors.lastname: Xin
authors.forename: Hui
authors.initials: H
authors.lastname: Xie
authors.forename: Qiong
authors.initials: Q
authors.lastname: Meng
authors.forename: Dong
authors.initials: D
authors.lastname: Jin
authors.forename: Bo
authors.initials: B
authors.lastname: Yu
authors.forename: Hui
authors.initials: H
authors.lastname: Sun
authors.forename: Shan
authors.initials: S
authors.lastname: Lu
authors.forename: Jianguo
authors.initials: J
authors.lastname: Xu
authors.forename: Yanwen
authors.initials: Y
authors.lastname: Xiong
mesh_terms: D000818:Animals; D002417:Cattle; D002681:China; D016521:Electrophoresis, Gel, Pulsed-Field; D004927:Escherichia coli Infections / Q000382:microbiology*; D029968:Escherichia coli Proteins / Q000235:genetics*; D005243:Feces / Q000382:microbiology; D060888:Real-Time Polymerase Chain Reaction; D012703:Serotyping; D054323:Shiga-Toxigenic Escherichia coli / Q000145:classification / Q000235:genetics*
chemical_list: D029968:Escherichia coli Proteins