title: The influence of matrix degradation and functionality on cell survival and morphogenesis in PEG-based hydrogels.
abstract: Two norbornene-functionalized PEG macromers are synthesized to render hydrogels with different hydrolytic degradability. Dithiol-containing linkers such as dithiothreitol or biscysteine-containing peptides are used to control proteolytic degradability. The influence of thiol-ene gel degradability on cell survival and morphogenesis in 3D is assessed using hMSCs and pancreatic MIN6 β cells. The initial cell viability can be negatively affected in highly crosslinked thiol-ene hydrogels. When cells are encapsulated in thiol-ene gels lacking cell-adhesive motifs, their survival and proliferation are promoted in more hydrolytically labile hydrogels. The degree of 3D cell spreading in encapsulated hMSCs is enhanced when the matrices are immobilized with cell-adhesive motifs.
authors.affiliation: Department of Biomedical Engineering, Purdue School of Engineering and Technology, Indiana University-Purdue University at Indianapolis, Indianapolis, IN 46202, USA.
authors.forename: Asad
authors.initials: A
authors.lastname: Raza
authors.forename: Chien-Chi
authors.initials: CC
authors.lastname: Lin
mesh_terms: D000818:Animals; D001672:Biocompatible Materials; D002448:Cell Adhesion; D002460:Cell Line; D002470:Cell Survival; D060833:Cellular Microenvironment; D005109:Extracellular Matrix / Q000378:metabolism*; D006801:Humans; D020100:Hydrogels / Q000378:metabolism*; D050417:Insulin-Secreting Cells / Q000378:metabolism*; D059630:Mesenchymal Stem Cells / Q000378:metabolism*; D051379:Mice; D009024:Morphogenesis; D009636:Norbornanes / Q000737:chemistry; D011092:Polyethylene Glycols / Q000737:chemistry*; D059748:Proteolysis; D023822:Tissue Engineering / Q000379:methods
chemical_list: D001672:Biocompatible Materials; D020100:Hydrogels; D009636:Norbornanes; D011092:Polyethylene Glycols
keywords: cell/material interactions; degradation; hydrogels; mesenchymal stem cells