title: Development of a multivariate calibration model for the determination of dry extract content in Brazilian commercial bee propolis extracts through UV-Vis spectroscopy.
abstract: This study had the objective of determining the content of dry extract of commercial alcoholic extracts of bee propolis through Partial Least Squares (PLS) multivariate calibration and electronic spectroscopy. The PLS model provided a good prediction of dry extract content in commercial alcoholic extracts of bee propolis in the range of 2.7 a 16.8% (m/v), presenting the advantage of being less laborious and faster than the traditional gravimetric methodology. The PLS model was optimized with outlier detection tests according to the ASTM E 1655-05. In this study it was possible to verify that a centrifugation stage is extremely important in order to avoid the presence of waxes, resulting in a more accurate model. Around 50% of the analyzed samples presented content of dry extract lower than the value established by Brazilian legislation, in most cases, the values found were different from the values claimed in the product's label.
authors.affiliation: Departamento de Química, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil. barbeira@ufmg.br
authors.forename: Paulo J S
authors.initials: PJ
authors.lastname: Barbeira
authors.forename: Rosilene S N
authors.initials: RS
authors.lastname: Paganotti
authors.forename: Ariane A
authors.initials: AA
authors.lastname: Assimos
mesh_terms: D000818:Animals; D001516:Bees; D002138:Calibration; D016018:Least-Squares Analysis; D011429:Propolis / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*
chemical_list: D011429:Propolis
keywords: Outlier detection; Partial Least Squares; Propolis; Propolis dry extract