title: [Chromatographic evaluation of multi-mode retention behavior of thiol-ene click chemistry based beta-cyclodextrin stationary phase].
abstract: A native beta-cyclodextrin (beta-CD) stationary phase was prepared by covalent bonding of beta-CD on silica particles via thiol-ene click chemistry. The resulting beta-CD bonded silica (Click TE-CD) was characterized by elemental analysis, which proved the successful immobilization of beta-CD on the silica support with thiol-ene click chemistry. Click TE-CD was chromatographically evaluated with a set of flavone glycosides under hydrophilic interaction chromatography (HILIC) mode, reversed-phase chromatography (RPLC) mode and supercritical fluid chromatography (SFC) mode. The acetonitrile content dependent "U" retention curves indicated its HILIC/RPLC mixed-mode retention behavior. The difference of the separation selectivity between HILIC, RPLC and SFC was described as orthogonality by using geometric approach. The orthogonalities between HILIC/RPLC, HILIC/SFC, and RPLC/SFC reached 69. 8%, 50. 8% and 50. 8%, respectively. The separation of Chinese traditional medicine Lignum Dalbergia Odorifera extract under HILIC, RPLC and SFC modes indicated the potential of Click TE-CD stationary phase in the analysis of complex samples. The mixed-mode HPLC properties and excellent orthogonality demon strated its flexibility in HPLC method development and its great potential in two-dimensional liquid chromatography separation on one HPLC column by different separation modes.
authors.affiliation: Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.
authors.forename: Xuefeng
authors.initials: X
authors.lastname: Xu
authors.forename: Aijin
authors.initials: A
authors.lastname: Shen
authors.forename: Zhimou
authors.initials: Z
authors.lastname: Guo
authors.forename: Xinmiao
authors.initials: X
authors.lastname: Liang