title: Searching for an immunogenetic factor that will illuminate susceptibility to non-tuberculous mycobacterial disease.
abstract: The incidence of pulmonary non-tuberculous mycobacteria (NTM) disease in otherwise healthy adults is increasing as the population ages. The organisms are ubiquitous so susceptibility probably reflects a deficiency in a protective immune response. Here we investigate if singlenucleotide polymorphisms (SNP) affecting cytokines, chemokines and their receptors associate with pulmonary NTM disease. Samples from NTM patients (n=79) and healthy controls (n=188) were genotyped using TaqMan probes. Of the 16 SNPs assessed, IL28B-rs8099917*TG (rs8099917; P=0.01, OR=2.2), TNFA-1031*CC (rs1799964; p=0.02, OR=0.48) and IL10-1082*AA (rs1800896; P=0.001, OR=0.33) were significantly associated with NTM disease. IL28B-rs8099917 and IL10-1082 have been associated with perturbations of the Th1/Th2 balance, whilst TNFA-1031*CC associates with sensory neuropathy in HIV patients. IL10-1082 warrants further investigation because we observed high production of IL-10 in blood mononuclear cells from NTM patients.
authors.affiliation: School of Pathology and Laboratory Medicine, University of Western Australia, Perth, Australia.
authors.forename: Jacquita S
authors.initials: JS
authors.lastname: Affandi
authors.forename: Shona
authors.initials: S
authors.lastname: Hendry
authors.forename: Grant
authors.initials: G
authors.lastname: Waterer
authors.forename: Rachel
authors.initials: R
authors.lastname: Thomson
authors.forename: Hilary
authors.initials: H
authors.lastname: Wallace
authors.forename: Sally
authors.initials: S
authors.lastname: Burrows
authors.forename: Patricia
authors.initials: P
authors.lastname: Price
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000483:Alleles; D004198:Disease Susceptibility; D005260:Female; D005838:Genotype; D006801:Humans; D016753:Interleukin-10 / Q000235:genetics / Q000276:immunology; D008297:Male; D008875:Middle Aged; D009165:Mycobacterium Infections, Nontuberculous / Q000235:genetics* / Q000276:immunology*; D016017:Odds Ratio; D018410:Pneumonia, Bacterial / Q000235:genetics* / Q000276:immunology*; D020641:Polymorphism, Single Nucleotide; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000276:immunology
chemical_list: D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10