title: Strain-dependent augmentation of tight-junction barrier function in human primary epidermal keratinocytes by Lactobacillus and Bifidobacterium lysates.
abstract: In this study, we investigated whether probiotic lysates can modify the tight-junction function of human primary keratinocytes. The keratinocytes were grown on cell culture inserts and treated with lysates from Bifidobacterium longum, Lactobacillus plantarum, Lactobacillus reuteri, Lactobacillus fermentum, or Lactobacillus rhamnosus GG. With the exception of L. fermentum (which decreased cell viability), all strains markedly enhanced tight-junction barrier function within 24 h, as assessed by measurements of transepithelial electrical resistance (TEER). However, B. longum and L. rhamnosus GG were the most efficacious, producing dose-dependent increases in resistance that were maintained for 4 days. These increases in TEER correlated with elevated expression of tight-junction protein components. Neutralization of Toll-like receptor 2 abolished both the increase in TEER and expression of tight-junction proteins induced by B. longum, but not L. rhamnosus GG. These data suggest that some bacterial strains increase tight-junction function via modulation of protein components but the different pathways involved may vary depending on the bacterial strain.
authors.affiliation: Schools of Medicine, The University of Manchester, Manchester Academic Health Sciences Centre, Manchester, UnitedKingdom.
authors.forename: Reshma
authors.initials: R
authors.lastname: Sultana
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: McBain
authors.forename: Catherine A
authors.initials: CA
authors.lastname: O'Neill
mesh_terms: D001644:Bifidobacterium / Q000737:chemistry* / Q000145:classification / Q000235:genetics; D006801:Humans; D015603:Keratinocytes / Q000378:metabolism / Q000382:microbiology*; D007778:Lactobacillus / Q000737:chemistry* / Q000145:classification / Q000235:genetics; D019936:Probiotics / Q000008:administration & dosage* / Q000737:chemistry; D019108:Tight Junctions / Q000378:metabolism / Q000382:microbiology*; D051195:Toll-Like Receptor 2 / Q000378:metabolism
chemical_list: D051195:Toll-Like Receptor 2