title: MicroRNA-217, down-regulated in clear cell renal cell carcinoma and associated with lower survival, suppresses cell proliferation and migration.
abstract: Aberrantly expressed microRNAs (miRNAs) are frequently correlated with a variety of human cancers, including clear cell renal cell carcinoma (ccRCC). In this study, we determined the expression patterns of miR-217 in ccRCC, and tested its effect on cancer cell proliferation and migration. The expression levels of miR-217 were determined in 54 ccRCC samples using Real-Time qPCR. 786-O and ACHN cells were transfected with miR-217 mimics or miRNA mimics control. Cell proliferation and migration were evaluated by MTT assay and scratch-wound assay, respectively. We found that miR-217 was down-regulated in ccRCC compared to paired normal tissue. Lower miR-217 expression levels were associated with higher tumor grade and stage. All patients with high miR-217 expression survived 5 years, while with low miR-217 expression, only 40% survived. Cell proliferation inhibition and decreased motility were observed in cells transfected with the miR-217 mimics. In conclusion, miR-217 plays a tumor suppressor role in ccRCC.
authors.affiliation: Foshan Maternal and Child Health Care Hospital, Foshan, China.
authors.forename: H
authors.initials: H
authors.lastname: Li
authors.forename: J
authors.initials: J
authors.lastname: Zhao
authors.forename: J W
authors.initials: JW
authors.lastname: Zhang
authors.forename: Q Y
authors.initials: QY
authors.lastname: Huang
authors.forename: J Z
authors.initials: JZ
authors.lastname: Huang
authors.forename: L S
authors.initials: LS
authors.lastname: Chi
authors.forename: H J
authors.initials: HJ
authors.lastname: Tang
authors.forename: G Q
authors.initials: GQ
authors.lastname: Liu
authors.forename: D J
authors.initials: DJ
authors.lastname: Zhu
authors.forename: W M
authors.initials: WM
authors.lastname: Ma
mesh_terms: D014408:Biomarkers, Tumor / Q000032:analysis / Q000235:genetics; D002292:Carcinoma, Renal Cell / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D015536:Down-Regulation; D006801:Humans; D053208:Kaplan-Meier Estimate; D007680:Kidney Neoplasms / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D035683:MicroRNAs / Q000096:biosynthesis / Q000235:genetics*; D011379:Prognosis; D060888:Real-Time Polymerase Chain Reaction; D014162:Transfection
chemical_list: D014408:Biomarkers, Tumor; C553315:MIRN217 microRNA, human; D035683:MicroRNAs