title: Changes in the reproductive endocrine function in rat following intraovary microinjection of inhba overexpression lentivirus vectors.
abstract: OBJECTIVE
  To investigate the reproductive endocrine changes after inhba overexpression into rat ovary.
  
  
  METHODS
  Thirty Sprague-Dawley rats were randomly assigned to three groups. Inhba overexpression lentivirus vectors (LV-eGFP-inhba) were microinjected into rat ovary (INH group); Control animals received the same amount lentivirus vector empty (CON group) or LV-eGFP (GFP group). Antral follicle amount and diameter were counted and serum level of activin A, E2, P, FSH, and LH and the expression of ER-α, ER-β, PR, FSHR, and LHR were measured.
  
  
  RESULTS
  There was no significant difference among three groups in antral follicle amount; antral follicle diameter was increased in INH group rats compared with the other group rats. Serum levels of activin A, E2, P, and FSH were increased and LH was decreased in INH group rats compared with the other group rats. The mRNA and protein expression of ER-α, ER-β, FSHR was higher in INH group rats than that in the other group rats. There was no significant difference in mRNA and protein expression of PR among the three group rats, LHR expression was decreased in INH group rats compared with the other two group rats.
  
  
  CONCLUSIONS
  inhba overexpression in rat ovary in vivo may change reproductive endocrine function.
authors.affiliation: Department of Obstetrics and Gynecology, First Affiliated Hospital of Chongqing medical university, Chongqing, China.
authors.forename: Wang
authors.initials: W
authors.lastname: Jidong
authors.forename: Ma
authors.initials: M
authors.lastname: Xiaohui
authors.forename: Du
authors.initials: D
authors.lastname: Fang
authors.forename: Hu
authors.initials: H
authors.lastname: Xiaojing
authors.forename: Luo
authors.initials: L
authors.lastname: Shuang
authors.forename: Pan
authors.initials: P
authors.lastname: Hongjuan
authors.forename: Yao
authors.initials: Y
authors.lastname: Zhenwei
mesh_terms: D000818:Animals; D005260:Female; D015870:Gene Expression; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D028322:Inhibin-beta Subunits / Q000235:genetics*; D016086:Lentivirus; D008845:Microinjections; D006080:Ovarian Follicle / Q000033:anatomy & histology / Q000502:physiology*; D010053:Ovary; D051381:Rats; D017207:Rats, Sprague-Dawley; D055647:Rats, Transgenic; D012098:Reproduction / Q000502:physiology*
chemical_list: C103008:inhibin beta A subunit; D028322:Inhibin-beta Subunits