title: Polyelectrolyte/silver nanocomposite multilayer films as multifunctional thin film platforms for remote activated protein and drug delivery.
abstract: We demonstrate a nanoparticle loading protocol to develop a transparent, multifunctional polyelectrolyte multilayer film for externally activated drug and protein delivery. The composite film was designed by alternate adsorption of poly(allylamine hydrochloride) (PAH) and dextran sulfate (DS) on a glass substrate followed by nanoparticle synthesis through a polyol reduction method. The films showed a uniform distribution of spherical silver nanoparticles with an average diameter of 50±20 nm, which increased to 80±20 nm when the AgNO3 concentration was increased from 25 to 50 mM. The porous and supramolecular structure of the polyelectrolyte multilayer film was used to immobilize ciprofloxacin hydrochloride (CH) and bovine serum albumin (BSA) within the polymeric network of the film. When exposed to external triggers such as ultrasonication and laser light the loaded films were ruptured and released the loaded BSA and CH. The release of CH is faster than that of BSA due to a higher diffusion rate. Circular dichroism measurements confirmed that there was no significant change in the conformation of released BSA in comparison with native BSA. The fabricated films showed significant antibacterial activity against the bacterial pathogen Staphylococcus aureus. Applications envisioned for such drug-loaded films include drug and vaccine delivery through the transdermal route, antimicrobial or anti-inflammatory coatings on implants and drug-releasing coatings for stents.
authors.affiliation: SRM Research Institute, SRM University, Kattankulathur, Chennai 603203, India. Electronic address: rsanandhakumar@gmail.com.
authors.forename: S
authors.initials: S
authors.lastname: Anandhakumar
authors.forename: Ashok M
authors.initials: AM
authors.lastname: Raichur
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002417:Cattle; D002448:Cell Adhesion / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002522:Chlorocebus aethiops; D002939:Ciprofloxacin / Q000494:pharmacology*; D002942:Circular Dichroism; D016503:Drug Delivery Systems; D004573:Electrolytes / Q000737:chemistry*; D004847:Epithelial Cells / Q000166:cytology / Q000187:drug effects; D008027:Light; D008826:Microbial Sensitivity Tests; D018613:Microscopy, Confocal; D053761:Nanocomposites / Q000737:chemistry* / Q000648:ultrastructure; D012710:Serum Albumin, Bovine / Q000378:metabolism*; D012834:Silver / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet; D013211:Staphylococcus aureus / Q000187:drug effects; D014465:Ultrasonics; D014709:Vero Cells
chemical_list: D000900:Anti-Bacterial Agents; D004573:Electrolytes; D012710:Serum Albumin, Bovine; D012834:Silver; D002939:Ciprofloxacin
keywords: Antibacterial studies; Composite multilayer films; Drug delivery; Protein delivery; Silver nanoparticles