title: Identification of European-type hepatitis E virus subtype 3e isolates in Japanese wild boars: molecular tracing of HEV from swine to wild boars.
abstract: Nucleotide sequences of hepatitis E virus (HEV) isolates infecting wild boars in Mie prefecture, which is located in the central region of Japan and is far from the most prevalent regions of HEV infection in Japan, were determined and characterised. Among 144 serum samples of wild boars captured in Mie prefecture, 7 were positive for HEV-RNA. The nucleotide sequence of nearly the entire genome was determined for 4 of the 7 positive samples. Phylogenetic tree analyses indicated that 6 samples were subtype 3e and 1 was subtype 3a among the 7 isolates. We identified the indigenization of subtype 3e isolates in Japanese wild boars. Furthermore, 5 subtype 3e isolates were closely related and were located in the peripheral branch of subtype 3e isolates from European countries in the phylogenetic tree. The structure indicated that the ancestor of the 5 subtype 3e isolates originated in Europe. The phylogenetic structure and coalescent analyses suggested that the subtype 3e isolates entered Japan from Europe by importation of large-race pigs around 1966. The results also indicated that several lineages of subtype 3e expanded to a wide area of Japan around 1992 and 1 of the lineages was indigenized in wild boars in Mie prefecture between 1992 and 2009. The appearance of a wild boar cluster in the peripheral branch in the phylogenetic lineage may indicate the direction of gene flow of HEV subtype 3e from swine to wild boars. Clarification of the transmission direction or route should be helpful to prevent a future endemic or epidemic of HEV infection.
authors.affiliation: Department of Internal Medicine, Fujita Health University Nanakuri Sanatorium, Mie 514-1295, Japan. tanakano@sf.starcat.ne.jp
authors.forename: Tatsunori
authors.initials: T
authors.lastname: Nakano
authors.forename: Kazuaki
authors.initials: K
authors.lastname: Takahashi
authors.forename: Masahiro
authors.initials: M
authors.lastname: Arai
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Okano
authors.forename: Hideaki
authors.initials: H
authors.lastname: Kato
authors.forename: Minoru
authors.initials: M
authors.lastname: Ayada
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Okamoto
authors.forename: Shunji
authors.initials: S
authors.lastname: Mishiro
mesh_terms: D000818:Animals; D000835:Animals, Wild; D016000:Cluster Analysis; D005838:Genotype; D016751:Hepatitis E / Q000662:veterinary* / Q000821:virology; D016752:Hepatitis E virus / Q000145:classification* / Q000235:genetics; D007564:Japan; D010802:Phylogeny; D058974:Phylogeography; D034421:Sus scrofa; D013552:Swine; D013553:Swine Diseases / Q000821:virology*
keywords: BF; Bayes factor; Genotype; HEV; Hepatitis E virus; Japan; MCMC; Markov chain Monte Carlo; Phylogenetic analysis; Subtype 3e; TMRCA; Wild boar; hepatitis E virus; time of the most recent common ancestor