title: Tracking down biotransformation to the genetic level: identification of a highly flexible glycosyltransferase from Saccharothrix espanaensis.
abstract: Saccharothrix espanaensis is a member of the order Actinomycetales. The genome of the strain has been sequenced recently, revealing 106 glycosyltransferase genes. In this paper, we report the detection of a glycosyltransferase from Saccharothrix espanaensis which is able to rhamnosylate different phenolic compounds targeting different positions of the molecules. The gene encoding the flexible glycosyltransferase is not located close to a natural product biosynthetic gene cluster. Therefore, the native function of this enzyme might be not the biosynthesis of a secondary metabolite but the glycosylation of internal and external natural products as part of a defense mechanism.
authors.affiliation: Albert Ludwigs University of Freiburg, Department of Pharmaceutical Biology and Biotechnology, Freiburg, Germany.
authors.forename: Tina
authors.initials: T
authors.lastname: Strobel
authors.forename: Yvonne
authors.initials: Y
authors.lastname: Schmidt
authors.forename: Anton
authors.initials: A
authors.lastname: Linnenbrink
authors.forename: Andriy
authors.initials: A
authors.lastname: Luzhetskyy
authors.forename: Marta
authors.initials: M
authors.lastname: Luzhetska
authors.forename: Takaaki
authors.initials: T
authors.lastname: Taguchi
authors.forename: Elke
authors.initials: E
authors.lastname: Brötz
authors.forename: Thomas
authors.initials: T
authors.lastname: Paululat
authors.forename: Maryna
authors.initials: M
authors.lastname: Stasevych
authors.forename: Oleg
authors.initials: O
authors.lastname: Stanko
authors.forename: Volodymyr
authors.initials: V
authors.lastname: Novikov
authors.forename: Andreas
authors.initials: A
authors.lastname: Bechthold
mesh_terms: D000192:Actinomycetales / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism*; D001711:Biotransformation; D002851:Chromatography, High Pressure Liquid; D017353:Gene Deletion; D016695:Glycosyltransferases / Q000235:genetics* / Q000378:metabolism*; D013058:Mass Spectrometry; D010636:Phenols / Q000378:metabolism; D010802:Phylogeny; D012210:Rhamnose / Q000378:metabolism; D017386:Sequence Homology, Amino Acid
chemical_list: D010636:Phenols; D016695:Glycosyltransferases; D012210:Rhamnose