title: The effect of initial purity on the stability of solutions in storage.
abstract: Many modern compound-screening technologies are highly miniaturized, resulting in longer-lasting solution stocks in compound management laboratories. As the ages of some stocks stretch into years, it becomes increasingly important to ensure that the DMSO solutions remain of high quality. It can be a burden to check the quality of a large library of compound solutions continuously, and so a study was devised to link the effects of initial compound purity and physicochemical properties of the compounds with the current purity of DMSO solutions. Approximately 5000 compounds with initial purity of at least 80% were examined. Storage conditions were held or observed to be relatively constant and so were eliminated as potential predictors. This allowed the evaluation of the effects of other factors on the stability of solutions, such as initial purity, number of freeze-thaw cycles, age of the solution, and multiple calculated physicochemical parameters. Of all the factors investigated, initial purity was the only one that had a clear effect on stability. None of the other parameters investigated (physicochemical properties, number of freeze-thaw cycles, age of solutions) had a statistically significant effect on stability.
authors.affiliation: 1GlaxoSmithKline, Research Triangle Park, NC, USA.
authors.forename: Ioana
authors.initials: I
authors.lastname: Popa-Burke
authors.forename: Steven
authors.initials: S
authors.lastname: Novick
authors.forename: Charles A
authors.initials: CA
authors.lastname: Lane
authors.forename: Robin
authors.initials: R
authors.lastname: Hogan
authors.forename: Pedro
authors.initials: P
authors.lastname: Torres-Saavedra
authors.forename: Brian
authors.initials: B
authors.lastname: Hardy
authors.forename: Brenda
authors.initials: B
authors.lastname: Ray
authors.forename: Melissa
authors.initials: M
authors.lastname: Lindsay
authors.forename: Iris
authors.initials: I
authors.lastname: Paulus
authors.forename: Luke
authors.initials: L
authors.lastname: Miller
mesh_terms: D004121:Dimethyl Sulfoxide / Q000737:chemistry; D004355:Drug Stability; D004356:Drug Storage; D006801:Humans; D012996:Solutions / Q000737:chemistry*
chemical_list: D012996:Solutions; D004121:Dimethyl Sulfoxide
keywords: purity; stability; storage