title: Regulation of miR-17-92a cluster processing by the microRNA binding protein SND1.
abstract: MicroRNAs are small non-coding RNAs that regulate gene expression. Although all seven members of the miR-17-92a cluster originate from one primary transcript they are differentially expressed suggesting the presence of posttranscriptional regulation. By RNA pulldown and mass spectrometry we identified SND1, a known regulator of edited RNAs, interacting with pre-miR-92a and all mature miR-17-92a members. Hypoxic conditions lead to an elevation of the pri-miR-17-92a transcript and significantly increased levels of the precursors whereas the mature miRs were not significantly changed. SND1 silencing resolved this block in processing and induced an increase in mature miRs. Together, SND1 might be the missing link between hypoxia and the differential regulation of miRNA processing.
authors.affiliation: Institute of Cardiovascular Regeneration, Center for Molecular Medicine, Frankfurt, Germany.
authors.forename: Eva-Marie
authors.initials: EM
authors.lastname: Heinrich
authors.forename: Jasmin
authors.initials: J
authors.lastname: Wagner
authors.forename: Marcus
authors.initials: M
authors.lastname: Krüger
authors.forename: David
authors.initials: D
authors.lastname: John
authors.forename: Shizuka
authors.initials: S
authors.lastname: Uchida
authors.forename: Julia E
authors.initials: JE
authors.lastname: Weigand
authors.forename: Beatrix
authors.initials: B
authors.lastname: Suess
authors.forename: Stefanie
authors.initials: S
authors.lastname: Dimmeler
mesh_terms: D002478:Cells, Cultured; D017931:DNA Primers; D004720:Endonucleases; D006801:Humans; D035683:MicroRNAs / Q000378:metabolism*; D009687:Nuclear Proteins / Q000378:metabolism*; D016133:Polymerase Chain Reaction
chemical_list: D017931:DNA Primers; C524941:MIRN17 microRNA, human; C541874:MIRN92 microRNA, human; D035683:MicroRNAs; D009687:Nuclear Proteins; D004720:Endonucleases; C095221:SND1 protein, human
keywords: Endothelial; MicroRNA; Processing; Tudor-SN