title: A quantitative ratiometric sensor for time-resolved analysis of auxin dynamics.
abstract: Time-resolved quantitative analysis of auxin-mediated processes in plant cells is as of yet limited. By applying a synergistic mammalian and plant synthetic biology approach, we have developed a novel ratiometric luminescent biosensor with wide applicability in the study of auxin metabolism, transport, and signalling. The sensitivity and kinetic properties of our genetically encoded biosensor open new perspectives for the analysis of highly complex auxin dynamics in plant growth and development.
authors.affiliation: Faculty of Biology, University of Freiburg, Freiburg, Germany.
authors.forename: Sabrina
authors.initials: S
authors.lastname: Wend
authors.forename: Cristina
authors.initials: C
authors.lastname: Dal Bosco
authors.forename: Michael M
authors.initials: MM
authors.lastname: Kämpf
authors.forename: Fugang
authors.initials: F
authors.lastname: Ren
authors.forename: Klaus
authors.initials: K
authors.lastname: Palme
authors.forename: Wilfried
authors.initials: W
authors.lastname: Weber
authors.forename: Alexander
authors.initials: A
authors.lastname: Dovzhenko
authors.forename: Matias D
authors.initials: MD
authors.lastname: Zurbriggen
mesh_terms: D015374:Biosensing Techniques; D002460:Cell Line; D006801:Humans; D007210:Indoleacetic Acids / Q000032:analysis*; D007700:Kinetics; D057230:Limit of Detection; D010944:Plants / Q000737:chemistry*
chemical_list: D007210:Indoleacetic Acids