title: A new cryptic virus belonging to the family Partitiviridae was found in watermelon co-infected with Melon necrotic spot virus.
abstract: A novel virus was detected in watermelon plants (Citrullus lanatus Thunb.) infected with Melon necrotic spot virus (MNSV) using SOLiD next-generation sequence analysis. In addition to the expected MSNV genome, two double-stranded RNA (dsRNA) segments of 1,312 and 1,118 bp were also identified and sequenced from the purified virus preparations. These two dsRNA segments encode two putative partitivirus-related proteins, an RNA-dependent RNA polymerase (RdRP) and a capsid protein, which were sequenced. Genomic-sequence analysis and analysis of phylogenetic relationships indicate that these two dsRNAs together make up the genome of a novel Partitivirus. This virus was found to be closely related to the Pepper cryptic virus 1 and Raphanus sativus cryptic virus. It is suggested that this novel virus putatively named Citrullus lanatus cryptic virus be considered as a new member of the family Partitiviridae.
authors.affiliation: Department of Plant Pathology, Agricultural Research Organization (ARO)-The Volcani Center, 50250, Bet Dagan, Israel, noa@volcani.agri.gov.il.
authors.forename: Noa
authors.initials: N
authors.lastname: Sela
authors.forename: Oded
authors.initials: O
authors.lastname: Lachman
authors.forename: Victoria
authors.initials: V
authors.lastname: Reingold
authors.forename: Aviv
authors.initials: A
authors.lastname: Dombrovsky
mesh_terms: D029773:Citrullus / Q000821:virology*; D016000:Cluster Analysis; D060085:Coinfection / Q000821:virology*; D059014:High-Throughput Nucleotide Sequencing; D008969:Molecular Sequence Data; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology*; D010942:Plant Viruses / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D012328:RNA Viruses / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D012367:RNA, Viral / Q000235:genetics; D017385:Sequence Homology; D014764:Viral Proteins / Q000235:genetics; D014780:Viruses
chemical_list: D012367:RNA, Viral; D014764:Viral Proteins