title: Increasing the electron-transfer ability of Cyanidioschyzon merolae ferredoxin by a one-point mutation--a high resolution and Fe-SAD phasing crystal structure analysis of the Asp58Asn mutant.
abstract: Cyanidioschyzon merolae (Cm) is a single cell red algae that grows in rather thermophilic (40-50°C) and acidic (pH 1-3) conditions. Ferredoxin (Fd) was purified from this algae and characterized as a plant-type [2Fe-2S] Fd by physicochemical techniques. A high resolution (0.97Å) three-dimensional structure of the CmFd D58N mutant molecule has been determined using the Fe-SAD phasing method to clarify the precise position of the Asn58 amide, as this substitution increases the electron-transfer ability relative to wild-type CmFd by a factor of 1.5. The crystal structure reveals an electro-positive surface surrounding Asn58 that may interact with ferredoxin NADP(+) reductase or cytochrome c.
authors.affiliation: Department of Life Science and Graduate School of Life Science, Rikkyo (St. Paul's ) University, Toshima-ku, Tokyo 171-8501, Japan.
authors.forename: Yuko
authors.initials: Y
authors.lastname: Ueno
authors.forename: Takashi
authors.initials: T
authors.lastname: Matsumoto
authors.forename: Akihito
authors.initials: A
authors.lastname: Yamano
authors.forename: Takeo
authors.initials: T
authors.lastname: Imai
authors.forename: Yukio
authors.initials: Y
authors.lastname: Morimoto
mesh_terms: D001216:Asparagine / Q000235:genetics*; D001224:Aspartic Acid / Q000235:genetics*; D018360:Crystallography, X-Ray; D004579:Electron Transport; D005288:Ferredoxins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D008958:Models, Molecular; D009154:Mutation; D011487:Protein Conformation; D000461:Rhodophyta / Q000378:metabolism*
chemical_list: D005288:Ferredoxins; D001224:Aspartic Acid; D001216:Asparagine
keywords: Electron transfer ability; High resolution analysis; Plant type ferredoxin; X-ray analysis