title: MiR-196a2 rs11614913 T > C polymorphism and risk of esophageal cancer in a Chinese population.
abstract: BACKGROUND
  Esophageal cancer is the eighth most common cancer and sixth leading cause of cancer associated death worldwide. Besides environmental risk factors, genetic factors might play an important role in the esophageal cancer carcinogenesis.
  
  
  METHODS
  We conducted a hospital based case-control study to evaluate the genetic susceptibility of functional single nucleotide polymorphisms (SNPs) in the microRNAs on the development of esophageal cancer. A total of 380 esophageal squamous cell carcinoma (ESCC) cases and 380 controls were recruited for this study. The miR-196a2 rs11614913 T > C, miR-146a rs2910164 C > G, miR-499 rs3746444 T > C, miR-26a-1 rs7372209 C > T and miR-27a rs895819 T > C genotypes were determined using a custom-by-design 48-Plex SNPscan™ Kit and matrix assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS).
  
  
  RESULTS
  MiR-196a2 rs11614913 T > C polymorphism was associated with borderline statistically decreased risk of ESCC. In the recessive model, when the miR-196a2 rs11614913 TT/TC genotypes were used as the reference group, the CC homozygote genotype was associated with a borderline statistically decreased risk for ESCC (adjusted OR 0.72, 95% CI 0.50-1.03, p = 0.070). In stratification analyses, a significantly decreased risk of ESCC associated with the miR-196a2 rs11614913 T > C polymorphism was evident among women patients and patients who never smoking or drinking.
  
  
  CONCLUSIONS
  These findings indicated that functional polymorphism miR-196a2 rs11614913 T > C might contribute to decreased ESCC risk among women patients and patients who never smoking or drinking. However, our results were obtained with a limited sample size. Future larger studies with other ethnic populations and tissue-specific biological characterization are required to confirm current findings.
authors.affiliation: Department of General Surgery, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China.
authors.forename: Jishu
authors.initials: J
authors.lastname: Wei
authors.forename: Liang
authors.initials: L
authors.lastname: Zheng
authors.forename: Shenghua
authors.initials: S
authors.lastname: Liu
authors.forename: Jun
authors.initials: J
authors.lastname: Yin
authors.forename: Liming
authors.initials: L
authors.lastname: Wang
authors.forename: Xu
authors.initials: X
authors.lastname: Wang
authors.forename: Yijun
authors.initials: Y
authors.lastname: Shi
authors.forename: Aizhong
authors.initials: A
authors.lastname: Shao
authors.forename: Weifeng
authors.initials: W
authors.lastname: Tang
authors.forename: Guowen
authors.initials: G
authors.lastname: Ding
authors.forename: Chao
authors.initials: C
authors.lastname: Liu
authors.forename: Suocheng
authors.initials: S
authors.lastname: Chen
authors.forename: Haiyong
authors.initials: H
authors.lastname: Gu
mesh_terms: D000368:Aged; D000428:Alcohol Drinking; D002294:Carcinoma, Squamous Cell / Q000235:genetics*; D016022:Case-Control Studies; D002681:China; D004938:Esophageal Neoplasms / Q000235:genetics*; D005260:Female; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D009367:Neoplasm Staging; D020641:Polymorphism, Single Nucleotide; D012306:Risk; D012737:Sex Factors; D012907:Smoking; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: C542543:MIRN196 microRNA, human; D035683:MicroRNAs
keywords: CI; LD; OR; SNPs; confidential interval; linkage disequilibrium; miRNA; microRNA; odds ratio; single nucleotide polymorphisms