title: Peanut sprout ethanol extract inhibits the adipocyte proliferation, differentiation, and matrix metalloproteinases activities in mouse fibroblast 3T3-L1 preadipocytes.
abstract: 3T3-L1 preadipocyte were differentiated to adipocytes, and then treated with 0, 10, 20, and 40 µg/mL of peanut sprout ethanol extract (PSEE). The main component of PSEE is resveratrol which contained 5.55 mg/mL of resveratrol. The MTT assay, Oil-Red O staining, glycerol-3-phosphate dehydrogenase (GPDH) activity, and the triglyceride concentration were determined in 3T3-L1 cells. MMP-2 and MMP-9 activities as well as mRNA expressions of C/EBP β and C/EBP α were also investigated. As the concentration of PSEE in adipocytes increased, the cell proliferation was decreased in a dose-dependent manner from 4 days of incubation (P < 0.05). The GDPH activity (P < 0.05) and the triglyceride concentration (P < 0.05) were decreased as the PSEE treatment concentration increased. The mRNA expression of C/EBPβ in 3T3-L1 cells was significantly low in groups of PSEE-treated, compared with control group (P < 0.05). The MMP-9 (P < 0.05) and MMP-2 (P < 0.05) activities were decreased in a dose-dependent manner as the PSEE concentration increased from 20 µg/mL. In conclusion, it was found that PSEE has an effect on restricting proliferation and differentiation of adipocytes.
authors.affiliation: Department of Food Science and Nutrition, Dankook University, 126 Jukjeon-dong, Suji-gu, Yongin-si, Gyeonggi 448-701, Korea.
authors.forename: Woo Kyoung
authors.initials: WK
authors.lastname: Kim
authors.forename: Nam E
authors.initials: NE
authors.lastname: Kang
authors.forename: Myung Hwan
authors.initials: MH
authors.lastname: Kim
authors.forename: Ae Wha
authors.initials: AW
authors.lastname: Ha
keywords: Peanut sprout extract; adipocytes; glycerol-3-phosphate dehydrogenase; matrix metalloproteinases; resveratrol