title: Influence of abiotic variables on culturable yeast diversity in two distinct Alpine glaciers.
abstract: The influence of some abiotic variables (pH, dry weight, organic carbon, nitrogen and phosphorous) on culturable yeast diversity in two distinct, but adjacent Alpine glaciers (Glacier du Géant, France, and Miage Glacier, Italy) was investigated. In all, 682 yeast strains were isolated and identified by D1/D2 and ITS sequencing as belonging to species of the genera Aureobasidium, Candida, Bulleromyces, Cryptococcus, Cystofilobasidium, Dioszegia, Guehomyces, Holtermanniella, Leucosporidiella, Mrakia, Mrakiella, Rhodotorula, Sporidiobolus, Sporobolomyces and Udenyomyces. Overall, the most represented genera were Cryptococcus (55% of isolates), Rhodotorula (17%) and Mrakia (10%). About 10% of strains, presumably belonging to new species (yet to be described), were preliminarily identified at the genus level. Principal component analysis (PCA) revealed that organic carbon, nitrogen and phosphorous are apparently mostly related to culturable yeast abundance and diversity. In this context, the hypothesis that the frequency of isolation of certain species may be correlated with some organic nutrients (with special emphasis for phosphorous) is discussed.
authors.affiliation: Department of Agricultural, Environmental and Food Science & Industrial Yeasts Collection DBVPG, University of Perugia, Perugia, Italy.
authors.forename: Benedetta
authors.initials: B
authors.lastname: Turchetti
authors.forename: Marta
authors.initials: M
authors.lastname: Goretti
authors.forename: Eva
authors.initials: E
authors.lastname: Branda
authors.forename: Guglielmina
authors.initials: G
authors.lastname: Diolaiuti
authors.forename: Carlo
authors.initials: C
authors.lastname: D'Agata
authors.forename: Claudio
authors.initials: C
authors.lastname: Smiraglia
authors.forename: Andrea
authors.initials: A
authors.lastname: Onofri
authors.forename: Pietro
authors.initials: P
authors.lastname: Buzzini
mesh_terms: D001487:Basidiomycota / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D044822:Biodiversity; D003081:Cold Climate; D005602:France; D046448:Ice Cover / Q000382:microbiology*; D007558:Italy; D010802:Phylogeny; D015003:Yeasts / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification
keywords: Alpine glaciers; PCA; abiotic variables; culturable yeast diversity; in situ enrichment