title: The P6 protein of Cauliflower mosaic virus interacts with CHUP1, a plant protein which moves chloroplasts on actin microfilaments.
abstract: The gene VI product, protein 6 (P6), of Cauliflower mosaic virus (CaMV) assembles into large, amorphous inclusion bodies (IBs) that are considered sites for viral protein synthesis and viral genome replication and encapsidation. P6 IBs align with microfilaments and require them for intracellular trafficking, a result implying that P6 IBs function to move virus complexes or virions within the cell to support virus physiology. Through a yeast two-hybrid screen we determined that CHUP1, a plant protein allowing chloroplast transport through an interaction with chloroplast and microfilament, interacts with P6. The interaction between CHUP1 and P6 was confirmed through colocalization in vivo and co-immunoprecipitation assays. A truncated CHUP1 fused with enhanced cyan fluorescent protein, unable to transport chloroplasts, inhibited intracellular movement of P6-Venus inclusions. Silencing of CHUP1 in N. edwardsonii impaired the ability of CaMV to infect plants. The findings suggest that CHUP1 supports CaMV infection through an interaction with P6.
authors.affiliation: Division of Plant Sciences, University of Missouri, Columbia, MO 65211, USA.
authors.forename: Carlos A
authors.initials: CA
authors.lastname: Angel
authors.forename: Lindy
authors.initials: L
authors.lastname: Lutz
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Yang
authors.forename: Andres
authors.initials: A
authors.lastname: Rodriguez
authors.forename: Adam
authors.initials: A
authors.lastname: Adair
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhang
authors.forename: Scott M
authors.initials: SM
authors.lastname: Leisner
authors.forename: Richard S
authors.initials: RS
authors.lastname: Nelson
authors.forename: James E
authors.initials: JE
authors.lastname: Schoelz
mesh_terms: D008841:Actin Cytoskeleton / Q000378:metabolism*; D017796:Caulimovirus / Q000235:genetics / Q000378:metabolism / Q000472:pathogenicity*; D060365:Chloroplast Proteins / Q000235:genetics / Q000378:metabolism*; D002736:Chloroplasts / Q000378:metabolism* / Q000821:virology; D047468:Immunoprecipitation; D008840:Microfilament Proteins / Q000235:genetics / Q000378:metabolism*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism*; D020798:Two-Hybrid System Techniques; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D060365:Chloroplast Proteins; D008840:Microfilament Proteins; D010940:Plant Proteins; D015534:Trans-Activators; D014764:Viral Proteins; C095687:gene VI protein, Cauliflower mosaic virus
keywords: CaMV; Inclusion bodies; Intracellular movement; Subcellular localization