title: Multiplexed MS/MS for improved data-independent acquisition.
abstract: In mass spectrometry-based proteomics, data-independent acquisition (DIA) strategies can acquire a single data set useful for both identification and quantification of detectable peptides in a complex mixture. However, DIA data are noisy owing to a typical five- to tenfold reduction in precursor selectivity compared to data obtained with data-dependent acquisition or selected reaction monitoring. We demonstrate a multiplexing strategy, MSX, for DIA analysis that increases precursor selectivity fivefold.
authors.affiliation: Department of Genome Sciences, University of Washington School of Medicine, Seattle, Washington, USA.
authors.forename: Jarrett D
authors.initials: JD
authors.lastname: Egertson
authors.forename: Andreas
authors.initials: A
authors.lastname: Kuehn
authors.forename: Gennifer E
authors.initials: GE
authors.lastname: Merrihew
authors.forename: Nicholas W
authors.initials: NW
authors.lastname: Bateman
authors.forename: Brendan X
authors.initials: BX
authors.lastname: MacLean
authors.forename: Ying S
authors.initials: YS
authors.lastname: Ting
authors.forename: Jesse D
authors.initials: JD
authors.lastname: Canterbury
authors.forename: Donald M
authors.initials: DM
authors.lastname: Marsh
authors.forename: Markus
authors.initials: M
authors.lastname: Kellmann
authors.forename: Vlad
authors.initials: V
authors.lastname: Zabrouskov
authors.forename: Christine C
authors.initials: CC
authors.lastname: Wu
authors.forename: Michael J
authors.initials: MJ
authors.lastname: MacCoss
mesh_terms: D010455:Peptides / Q000032:analysis*; D040901:Proteomics / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D010455:Peptides