title: Structurally diverse chaetophenol productions induced by chemically mediated epigenetic manipulation of fungal gene expression.
abstract: Epigenetic manipulation of gene expression in Chaetomium indicum using a HDAC inhibitor led to the isolation of structurally diverse chaetophenols, and 3, 4 and 5 bear unprecedented polycyclic skeletons. The expression of two silent genes (pksCH-1 and pksCH-2) for nonreducing PKSs involved in chaetophenol biosynthesis was associated with an increase of histone acetylation level. The heterologous gene expression study in Aspergillus oryzae revealed pksCH-2 to be the NR-PKS gene for 8.
authors.affiliation: Graduate School of Pharmaceutical Sciences, Tohoku University, Aoba-yama, Aoba-ku, Sendai 980-8578, Japan. tasai@m.tohoku.ac.jp
authors.forename: Teigo
authors.initials: T
authors.lastname: Asai
authors.forename: Takashi
authors.initials: T
authors.lastname: Yamamoto
authors.forename: Naoki
authors.initials: N
authors.lastname: Shirata
authors.forename: Tohru
authors.initials: T
authors.lastname: Taniguchi
authors.forename: Kenji
authors.initials: K
authors.lastname: Monde
authors.forename: Isao
authors.initials: I
authors.lastname: Fujii
authors.forename: Katsuya
authors.initials: K
authors.lastname: Gomi
authors.forename: Yoshiteru
authors.initials: Y
authors.lastname: Oshima
mesh_terms: D000107:Acetylation; D001236:Aspergillus oryzae / Q000737:chemistry* / Q000235:genetics; D001578:Benzopyrans / Q000138:chemical synthesis* / Q000737:chemistry; D002597:Chaetomium / Q000737:chemistry*; D003500:Cyclization; D057890:Epigenomics; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal / Q000235:genetics; D015394:Molecular Structure; D010636:Phenols / Q000138:chemical synthesis* / Q000737:chemistry*; D011083:Polycyclic Compounds / Q000138:chemical synthesis* / Q000737:chemistry*; D061065:Polyketides / Q000138:chemical synthesis* / Q000737:chemistry
chemical_list: D001578:Benzopyrans; D010636:Phenols; D011083:Polycyclic Compounds; D061065:Polyketides