title: Primary culture of breast cancer: a model system for epithelial-mesenchymal transition and cancer stem cells.
abstract: BACKGROUND
  A novel model system to study the cellular, molecular, and genetic characteristics of breast cancer stem cells is needed. Personalized prognostic models are indispensable for assessing the effect of so-called tailor-made adjuvant therapy.
  
  
  METHODS
  Surgically-extirpated tissues were dispersed with Dispase and cultured in commonly used medium. The expression of tumor markers was detected with immunohistochemistry, and gene expression profiles of tissues and cells were analyzed using the Agilent Human Microarray.
  
  
  RESULTS
  We established a primary culture, which exhibited mesenchymal morphology. The gene expression profile of the primary culture revealed that the cells underwent epithelial-mesenchymal transition and had cancer stem cell properties.
  
  
  CONCLUSIONS
  A primary culture of breast cancer cells can be easily established and could be used for studying breast cancer stem cells and assessing treatment of a patient.
authors.affiliation: Frontiers of Innovative Research in Science and Technology (FIRST), Konan University, 7-1-20 Minatojima-minamimachi, Chuo-ku, Kobe 605-0047, Japan. nisikata@konan-u.ac.jp
authors.forename: Takahito
authors.initials: T
authors.lastname: Nishikata
authors.forename: Mami
authors.initials: M
authors.lastname: Ishikawa
authors.forename: Tetsuhiro
authors.initials: T
authors.lastname: Matsuyama
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Takamatsu
authors.forename: Tosiyuki
authors.initials: T
authors.lastname: Fukuhara
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Konishi
mesh_terms: D002293:Adenocarcinoma, Scirrhous / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D014408:Biomarkers, Tumor / Q000235:genetics* / Q000378:metabolism; D001940:Breast / Q000378:metabolism / Q000473:pathology*; D001943:Breast Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D002478:Cells, Cultured; D058750:Epithelial-Mesenchymal Transition; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D008875:Middle Aged; D008954:Models, Biological; D014411:Neoplastic Stem Cells / Q000378:metabolism / Q000473:pathology*; D020411:Oligonucleotide Array Sequence Analysis; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D014408:Biomarkers, Tumor; D012333:RNA, Messenger
keywords: CD44; CSC; EMT; Epithelial-mesenchymal transition; cadherin; cancer stem cell; musashi