title: Hybridisation, paternal leakage and mitochondrial DNA linearization in three anomalous fish (Scombridae).
abstract: Using mitochondrial DNA for species identification and population studies assumes that the genome is maternally inherited, circular, located in the cytoplasm and lacks recombination. This study explores the mitochondrial genomes of three anomalous mackerel. Complete mitochondrial genome sequencing plus nuclear microsatellite genotyping of these fish identified them as Scomberomorus munroi (spotted mackerel). Unlike normal S. munroi, these three fish also contained different linear, mitochondrial genomes of Scomberomorus semifasciatus (grey mackerel). The results are best explained by hybridisation, paternal leakage and mitochondrial DNA linearization. This unusual observation may provide an explanation for mtDNA outliers in animal population studies.
authors.affiliation: Molecular Fisheries Laboratory, University of Queensland, PO Box 6097, St Lucia, Queensland 4072, Australia; Queensland Alliance for Agriculture and Food Innovation, University of Queensland, 306 Carmody Rd, St Lucia, Queensland 4072, Australia. Electronic address: Jessica.Morgan@uq.edu.au.
authors.forename: Jess A T
authors.initials: JA
authors.lastname: Morgan
authors.forename: Michael
authors.initials: M
authors.lastname: Macbeth
authors.forename: Damien
authors.initials: D
authors.lastname: Broderick
authors.forename: Paul
authors.initials: P
authors.lastname: Whatmore
authors.forename: Raewyn
authors.initials: R
authors.lastname: Street
authors.forename: David J
authors.initials: DJ
authors.lastname: Welch
authors.forename: Jennifer R
authors.initials: JR
authors.lastname: Ovenden
mesh_terms: D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D004272:DNA, Mitochondrial / Q000235:genetics*; D054629:Genome, Mitochondrial; D006824:Hybridization, Genetic; D010473:Perciformes / Q000145:classification / Q000235:genetics*; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D013045:Species Specificity
chemical_list: D017931:DNA Primers; D004272:DNA, Mitochondrial
keywords: Australia; Linear mtDNA; Mackerel; Nuclear mitochondrial DNA; Numt