title: Prevotella jejuni sp. nov., isolated from the small intestine of a child with coeliac disease.
abstract: Five obligately anaerobic, Gram-stain-negative, saccharolytic and proteolytic, non-spore-forming bacilli (strains CD3 : 27, CD3 : 28(T), CD3 : 33, CD3 : 32 and CD3 : 34) are described. All five strains were isolated from the small intestine of a female child with coeliac disease. Cells of the five strains were short rods or coccoid cells with longer filamentous forms seen sporadically. The organisms produced acetic acid and succinic acid as major metabolic end products. Phylogenetic analysis based on comparative 16S rRNA gene sequence analysis revealed close relationships between CD3 : 27, CD3 : 28(T) and CD3 : 33, between CD3 : 32 and Prevotella histicola CCUG 55407(T), and between CD3 : 34 and Prevotella melaninogenica CCUG 4944B(T). Strains CD3 : 27, CD3 : 28(T) and CD3 : 33 were clearly different from all recognized species within the genus Prevotella and related most closely to but distinct from P. melaninogenica. Based on 16S rRNA, RNA polymerase β-subunit (rpoB) and 60 kDa chaperonin protein subunit (cpn60) gene sequencing, and phenotypic, chemical and biochemical properties, strains CD3 : 27, CD3 : 28(T) and CD3 : 33 are considered to represent a novel species within the genus Prevotella, for which the name Prevotella jejuni sp. nov. is proposed. Strain CD3 : 28(T) ( = CCUG 60371(T) = DSM 26989(T)) is the type strain of the proposed novel species. All five strains were able to form homologous aggregates, in which tube-like structures were connecting individual bacteria cells. The five strains were able to bind to human intestinal carcinoma cell lines at 37 °C.
authors.affiliation: Department of Clinical Microbiology, Immunology, Umeå University, SE-90187 Umeå, Sweden.
authors.forename: Maria E
authors.initials: ME
authors.lastname: Hedberg
authors.affiliation: Department of Clinical Microbiology, Immunology, Umeå University, SE-90187 Umeå, Sweden.
authors.forename: Anne
authors.initials: A
authors.lastname: Israelsson
authors.affiliation: Department of Infectious Diseases, Sahlgrenska Academy of the University of Gothenburg, SE-40530 Göteborg, Sweden.|CCUG - Culture Collection University of Gothenburg, Department of Clinical Bacteriology, Sahlgrenska University Hospital, SE-41345 Göteborg, Sweden.
authors.forename: Edward R B
authors.initials: ERB
authors.lastname: Moore
authors.affiliation: CCUG - Culture Collection University of Gothenburg, Department of Clinical Bacteriology, Sahlgrenska University Hospital, SE-41345 Göteborg, Sweden.
authors.forename: Liselott
authors.initials: L
authors.lastname: Svensson-Stadler
authors.affiliation: Department of Molecular Biology, Umeå University, SE-90187 Umeå, Sweden.
authors.forename: Sun Nyunt
authors.initials: SN
authors.lastname: Wai
authors.affiliation: Department of Clinical Microbiology, Immunology, Umeå University, SE-90187 Umeå, Sweden.
authors.forename: Grzegorz
authors.initials: G
authors.lastname: Pietz
authors.affiliation: Department of Clinical Sciences, Pediatrics, Umeå University, SE-90187 Umeå, Sweden.
authors.forename: Olof
authors.initials: O
authors.lastname: Sandström
authors.affiliation: Department of Clinical Sciences, Pediatrics, Umeå University, SE-90187 Umeå, Sweden.
authors.forename: Olle
authors.initials: O
authors.lastname: Hernell
authors.affiliation: Department of Clinical Microbiology, Immunology, Umeå University, SE-90187 Umeå, Sweden.
authors.forename: Marie-Louise
authors.initials: ML
authors.lastname: Hammarström
authors.affiliation: Department of Clinical Microbiology, Immunology, Umeå University, SE-90187 Umeå, Sweden.
authors.forename: Sten
authors.initials: S
authors.lastname: Hammarström
mesh_terms: D019342:Acetic Acid / Q000378:metabolism; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002446:Celiac Disease / Q000382:microbiology*; D045744:Cell Line, Tumor; D002648:Child; D004269:DNA, Bacterial / Q000235:genetics; D012321:DNA-Directed RNA Polymerases / Q000235:genetics; D004847:Epithelial Cells / Q000382:microbiology; D005227:Fatty Acids / Q000737:chemistry; D005260:Female; D006801:Humans; D007421:Intestine, Small / Q000166:cytology / Q000382:microbiology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D018720:Prevotella / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D019802:Succinic Acid / Q000378:metabolism; D013548:Sweden
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D019802:Succinic Acid; D012321:DNA-Directed RNA Polymerases; C105078:RNA polymerase beta subunit; D019342:Acetic Acid