title: Semiquinone fraction isolated from Bacillus sp. INM-1 protects hepatic tissues against γ-radiation induced toxicity.
abstract: Present study was focused on evaluation of a semiquinone glucoside derivative (SQGD) isolated from radioresistant bacterium Bacillus sp. INM-1 for its ability against γ radiation induced oxidative stress in irradiated mice. Animals were divided into four group, i.e., (i) untreated control mice; (ii) SQGD treated (50 mg/kg b. wt. i.p.) mice; (iii) irradiated (10 Gy) mice; and (iv) irradiated mice which were pre-treated (-2 h) with SQGD (50 mg/kg b. wt. i.p.). Following treatment, liver homogenates of the treated mice were subjected to endogenous antioxidant enzymes estimation. Result indicated that SQGD pre-treatment, significantly (P < 0.05) induced superoxide dismutase (SOD) (19.84 ± 2.18% at 72 h), catalase (CAT) (26.47 ± 3.11% at 12 h), glutathione (33.81 ± 1.99% at 24 h), and glutathione-S-transferase (24.40 ± 2.65% at 6 h) activities in the liver of mice as compared with untreated control. Significant (P < 0.05) induction in SOD (50.04 ± 5.59% at 12 h), CAT (62.22 ± 7.50 at 72 h), glutathione (42.92 ± 2.28% at 24 h), and glutathione-S-transferase (46.65 ± 3.25 at 12 h) was observed in irradiated mice which were pre-treated with SQGD compared with only irradiated mice. Further, significant induction in ABTS(+) radicals (directly proportional to decrease mM Trolox equivalent) was observed in liver homogenate of H2 O2 treated mice which were found to be significantly inhibited in H2 O2 treated mice pre-treated with SQGD. Thus, it can be concluded that SQGD treatment neutralizes oxidative stress caused by irradiation not only by enhancing endogenous antioxidant enzymes but also by improving total antioxidant status of cellular system and thus cumulative effect of the phenomenon may contributes to radioprotection.
authors.affiliation: Radiation Biotechnology Laboratory, Department of Radiation Biosciences, Institute of Nuclear Medicine and Allied Sciences, Delhi, 110054, India.
authors.forename: Saurabh
authors.initials: S
authors.lastname: Mishra
authors.forename: Deen Dayal
authors.initials: DD
authors.lastname: Bansal
authors.forename: Poonam
authors.initials: P
authors.lastname: Malhotra
authors.forename: D Sudheer
authors.initials: DS
authors.lastname: K Reddy
authors.forename: Vishawdeep S
authors.initials: VS
authors.lastname: Jamwal
authors.forename: Dev Dutt
authors.initials: DD
authors.lastname: Patel
authors.forename: Ashutosh Kumar
authors.initials: AK
authors.lastname: Gupta
authors.forename: Praveen Kumar
authors.initials: PK
authors.lastname: Singh
authors.forename: Saleem
authors.initials: S
authors.lastname: Javed
authors.forename: Raj
authors.initials: R
authors.lastname: Kumar
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D001407:Bacillus / Q000737:chemistry; D016227:Benzoquinones / Q000302:isolation & purification / Q000494:pharmacology*; D002374:Catalase / Q000378:metabolism; D005720:Gamma Rays / Q000009:adverse effects*; D005960:Glucosides / Q000302:isolation & purification / Q000494:pharmacology*; D005978:Glutathione / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism / Q000528:radiation effects*; D008297:Male; D008810:Mice, Inbred C57BL; D018384:Oxidative Stress / Q000187:drug effects; D011837:Radiation-Protective Agents / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D000975:Antioxidants; D016227:Benzoquinones; D005960:Glucosides; D011837:Radiation-Protective Agents; D002374:Catalase; D013482:Superoxide Dismutase; D005982:Glutathione Transferase; D005978:Glutathione
keywords: GSH; antioxidant; catalase; glutathione-S-transferase; radioprotection; superoxide dismutase