title: Natural polyelectrolyte self-assembled multilayers based on collagen and alginate: stability and cytocompatibility.
abstract: Scientific interest in the self-assembly of collagen composite films has been increasing for their potential application in constructing bioactive materials. Here we report a highly stable and cytocompatible collagen/alginate (COL/ALG) ultrathin film, which was linearly fabricated via a layer-by-layer self-assembled technique. The variation in morphology and thickness of the films in air and in solutions with different pH and ion values were tested by atomic force microscopy. Results showed that the solutions with high pH values or solutions that contained electrolytes would disintegrate the film, while films with that were cross-linked for a long time prevented the dissolution and contributed to stability maintenance of the films. Interestingly, the COL/ALG coating not only improved the adhesion and proliferation of the human periodontal ligament cells, but also modified the morphology and migration of cells on the surface of glass and poly-L-lactic acid (PLA) electrospun scaffolds. In conclusion, the COL/ALG ultrathin films were highly stable and cytocompatible and could be easily fabricated by the cost-effective self-assembled technique presented. The findings of this study have the potential to play an important role in the surface modification of biomaterials.
authors.affiliation: Department of Orthodontics, School of Stomatology, Tongji University, Shanghai, China.
authors.forename: Wenxing
authors.initials: W
authors.lastname: Li
authors.forename: Peng
authors.initials: P
authors.lastname: Zhao
authors.forename: Chao
authors.initials: C
authors.lastname: Lin
authors.forename: Xuejun
authors.initials: X
authors.lastname: Wen
authors.forename: Eleni
authors.initials: E
authors.lastname: Katsanevakis
authors.forename: Decher
authors.initials: D
authors.lastname: Gero
authors.forename: Olivier
authors.initials: O
authors.lastname: Félix
authors.forename: Yuehua
authors.initials: Y
authors.lastname: Liu
mesh_terms: D000464:Alginates / Q000737:chemistry*; D002448:Cell Adhesion; D002465:Cell Movement; D048430:Cell Shape; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000737:chemistry; D004573:Electrolytes / Q000737:chemistry; D005260:Female; D024022:Fibrillar Collagens / Q000737:chemistry*; D005898:Glass / Q000737:chemistry; D006801:Humans; D006863:Hydrogen-Ion Concentration; D019344:Lactic Acid / Q000737:chemistry; D008422:Materials Testing; D018625:Microscopy, Atomic Force; D010513:Periodontal Ligament / Q000166:cytology; D011091:Polyesters; D011108:Polymers / Q000737:chemistry; D055550:Protein Stability; D012996:Solutions; D013499:Surface Properties; D054457:Tissue Scaffolds / Q000737:chemistry*; D055815:Young Adult
chemical_list: D000464:Alginates; D020099:Coated Materials, Biocompatible; D004573:Electrolytes; D024022:Fibrillar Collagens; D011091:Polyesters; D011108:Polymers; D012996:Solutions; D019344:Lactic Acid; C033616:poly(lactide)