title: DNA origami as a DNA repair nanosensor at the single-molecule level.
abstract: The folding of DNA molecules by DNA origami is used in a nanosensor to analyze enzymatic DNA repair activity of hAGT. The method uses conformational changes that condition α-thrombin interaction with DNA aptamers, and illustrates the use of DNA origami as a proteinrecognition biosensor.
authors.affiliation: IRB Barcelona, IQAC-CSIC, CIBER-BBN Networking Centre on Bioengineering Biomaterials and Nanomedicine c/Jordi Girona 18-26, 08034 Barcelona, Spain.
authors.forename: Maria
authors.initials: M
authors.lastname: Tintoré
authors.forename: Isaac
authors.initials: I
authors.lastname: Gállego
authors.forename: Brendan
authors.initials: B
authors.lastname: Manning
authors.forename: Ramon
authors.initials: R
authors.lastname: Eritja
authors.forename: Carme
authors.initials: C
authors.lastname: Fàbrega
mesh_terms: D000808:Angiotensinogen / Q000378:metabolism*; D052157:Aptamers, Nucleotide / Q000737:chemistry; D015374:Biosensing Techniques; D004260:DNA Repair; D006801:Humans; D018625:Microscopy, Atomic Force; D049329:Nanostructures / Q000737:chemistry; D036103:Nanotechnology; D013917:Thrombin / Q000378:metabolism*
chemical_list: C534931:AGT protein, human; D052157:Aptamers, Nucleotide; D000808:Angiotensinogen; D013917:Thrombin
keywords: DNA origami; DNA repair; G-quadruplexes; biosensors; hAGT