title: [Association study between PDE4D gene polymorphism and ischemic stroke].
abstract: OBJECTIVE
  To investigate the linkage and association between rs966221 (SNP 83) in PDE4D gene with stroke and related traits in ischemic stroke families.
  
  
  METHODS
  Ischemic stroke families including ischemic stroke patients and their siblings and/or parents were recruited. Generalized estimating equation (GEE) was used to adjust for with-in family correlations and other potential confounding factors. Non-parameter linkage analysis and family based association test (FBAT) were applied to explore the relationship between rs966221 polymorphism and ischemic stroke together with its related traits.
  
  
  RESULTS
  In the study 276 ischemic stroke families with totally 776 participants were enrolled. Apolipoprotein B (apoB), carotid intima media thickness (cIMT), high-density lipoprotein cholesterol and blood pressure were associated with ischemic stroke. In family based association test, after being adjusted for related chronic diseases, rs966221 C allele was found to be associated with cIMT in the dominant model (P=0.019), TT genotype (P=0.019) and CT genotype (P=0.007) were associated with cIMT significantly. After being adjusted for potential confounding factors, evidence of linkage was observed for rs966221 with apoB (P<0.001), high-sensitivity C-reactive protein (P=0.003) and systolic blood pressure (P=0.036).
  
  
  CONCLUSIONS
  Abnormal serum lipid, blood pressure and increasing cIMT were associated with ischemic stroke, and linkage was observed for with apoB, high-sensitivity C-reactive protein, and systolic blood pressure; rs966221 C allele was probably associated with cIMT.
authors.affiliation: Department of Epidemiology and Biostatistics, Peking University School of Public Health, Beijing, China.
authors.forename: Kuo
authors.initials: K
authors.lastname: Liu
authors.forename: Jin-wei
authors.initials: JW
authors.lastname: Wang
authors.forename: Zhi-ping
authors.initials: ZP
authors.lastname: Yu
authors.forename: Xue-ying
authors.initials: XY
authors.lastname: Qin
authors.forename: Yi-qun
authors.initials: YQ
authors.lastname: Wu
authors.forename: Na
authors.initials: N
authors.lastname: Li
authors.forename: Ying-qi
authors.initials: YQ
authors.lastname: Kui
authors.forename: Kai
authors.initials: K
authors.lastname: Fang
authors.forename: Xue-yin
authors.initials: XY
authors.lastname: Wang
authors.forename: Tao
authors.initials: T
authors.lastname: Wu
authors.forename: Da-fang
authors.initials: DF
authors.lastname: Chen
authors.forename: Yong-hua
authors.initials: YH
authors.lastname: Hu
mesh_terms: D000483:Alleles; D001055:Apolipoproteins B / Q000097:blood; D001794:Blood Pressure; D002545:Brain Ischemia / Q000150:complications; D002097:C-Reactive Protein / Q000378:metabolism; D059168:Carotid Intima-Media Thickness; D008076:Cholesterol, HDL / Q000097:blood; D054703:Cyclic Nucleotide Phosphodiesterases, Type 4 / Q000235:genetics*; D056726:Genetic Association Studies; D008040:Genetic Linkage; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D020641:Polymorphism, Single Nucleotide; D020521:Stroke / Q000209:etiology / Q000235:genetics*
chemical_list: D001055:Apolipoproteins B; D008076:Cholesterol, HDL; D002097:C-Reactive Protein; D054703:Cyclic Nucleotide Phosphodiesterases, Type 4; C517376:PDE4D protein, human