title: [Efficacy of iron saturated recombinant human lactoferrin on alleviating iron deficiency anemia in rats].
abstract: OBJECTIVE
  To investigate the effect of iron saturated recombinant human lactoferrin (Fe-rhLf) on treating iron deficiency anemia (IDA).
  
  
  METHODS
  SD rats received iron deficient diet and deionized water during the whole experiment. After 4 weeks, the rats with hemoglobin less than 100 g/L were selected and divided into 5 groups randomly. IDA group received 100 mg/(kg×d) casein dissolved in deionized water intragastrically; FeSO4 group received 5.43 mg/(kg×d) [2.0 mg/(kg×d) iron]+100 mg/(kg×d) casein intragastrically; low dose Fe-rhLf group received 75.53 mg/(kg×d) Fe-rhLf [0.5 mg/(kg×d) iron] +75 mg/(kg×d) casein intragastrically; middle dose group received 151.06 mg/(kg×d) Fe-rhLf [1.0 mg/(kg×d) iron]+50 mg/(kg×d) casein intragastrically; high dose group received 302.11 mg/(kg×d) Fe-rhLf [2.0 mg/(kg×d) iron] intragastrically. After 30 days of intervention, the rats' blood was collected and used to do the routine blood test, serum iron and serum anti-oxidants test. The liver hepcidin and ferroportin mRNA expression was tested by real time PCR.
  
  
  RESULTS
  Compared with the animals in the IDA group, Fe-rhLf could increase weight significantly (P<0.05), hematological parameters, like hemoglobin (P<0.01) and red blood cell count (P<0.01) recovered significantly. Compared with animals in FeSO4 group, high dose Fe-rhLf could improve hemoglobin significantly (P<0.05). The liver hepcidin expression was up regulated and ferroportin down regulated.
  
  
  CONCLUSIONS
  Fe-rhLf has a significant effect on treating iron deficiency anemia.
authors.affiliation: Department of Nutrition and Food Hygiene, Peking University School of Public Health, Beijing, China.
authors.forename: Yue-hui
authors.initials: YH
authors.lastname: Fang
authors.forename: Xin
authors.initials: X
authors.lastname: Feng
authors.forename: Hao
authors.initials: H
authors.lastname: Xu
authors.forename: Zheng-hao
authors.initials: ZH
authors.lastname: Wu
authors.forename: Ya-jun
authors.initials: YJ
authors.lastname: Xu
mesh_terms: D018798:Anemia, Iron-Deficiency / Q000628:therapy*; D000818:Animals; D006454:Hemoglobins; D006801:Humans; D007501:Iron; D007781:Lactoferrin / Q000494:pharmacology*; D008099:Liver; D051381:Rats; D017207:Rats, Sprague-Dawley; D011994:Recombinant Proteins / Q000494:pharmacology
chemical_list: D006454:Hemoglobins; D011994:Recombinant Proteins; D007501:Iron; D007781:Lactoferrin