title: Studies on insecticidal activities and action mechanism of novel benzoylphenylurea candidate NK-17.
abstract: Insecticidal activity of NK-17 was evaluated both in laboratory and in field. It was found that the toxicity of NK-17 against S. exigua was 1.93 times and 2.69 times those of hexaflumuron and chlorfluazuron respectively, and the toxicity of NK-17 against P. xylostella was 1.36 times and 1.90 times those of hexaflumuron and chlorfluazuron respectively, and the toxicity of NK-17 against M. separate was 18.24 times those of hexaflumuron in laboratory, and 5% NK-17 EC at 60 g a.i ha(-1) can control S. exigua and P. xylostella with the best control efficiency of about 89% and over 88% respectively in Changsha and Tianjin in field. The insecticidal mechanism of NK-17 was explored for the first time by utilizing the fluorescence polarization method. NK-17 could bind to sulfonylurea receptor (SUR) of B. germanica with stronger affinity comparing to diflubenzuron and glibenclamide, which suggested that NK-17 may also act on the site of SUR to inhibit the chitin synthesis in insect body and the result can well explain that NK-17 exhibited stronger toxicity against B. germanica than diflubenzuron and glibenclamide in vivo.
authors.affiliation: Key Laboratory of Pesticide Chemistry and Application, MOA, Department of Applied Chemistry, College of Science, China Agricultural University, Beijing, People's Republic of China.
authors.forename: Yongqiang
authors.initials: Y
authors.lastname: Li
authors.forename: Yaoguo
authors.initials: Y
authors.lastname: Qin
authors.forename: Na
authors.initials: N
authors.lastname: Yang
authors.forename: Yufeng
authors.initials: Y
authors.lastname: Sun
authors.forename: Xinling
authors.initials: X
authors.lastname: Yang
authors.forename: Ranfeng
authors.initials: R
authors.lastname: Sun
authors.forename: Qingmin
authors.initials: Q
authors.lastname: Wang
authors.forename: Yun
authors.initials: Y
authors.lastname: Ling
mesh_terms: D000818:Animals; D001549:Benzamides / Q000494:pharmacology; D004132:Diflubenzuron / Q000494:pharmacology; D005905:Glyburide / Q000494:pharmacology; D007313:Insecta / Q000187:drug effects / Q000378:metabolism; D007306:Insecticides / Q000494:pharmacology*; D009036:Moths / Q000187:drug effects; D010671:Phenylurea Compounds / Q000494:pharmacology; D011725:Pyridines / Q000494:pharmacology; D064233:Sulfonylurea Receptors / Q000378:metabolism; D005466:Fluorocarbons
chemical_list: D001549:Benzamides; D004132:Diflubenzuron; D005905:Glyburide; D007306:Insecticides; D010671:Phenylurea Compounds; D011725:Pyridines; D064233:Sulfonylurea Receptors; C058039:hexafluoron; D005466:Fluorocarbons; C057223:chlorfluazuron