title: A chemo-enzymatic approach for site-specific modification of the RNA cap.
abstract: Capped and gowned: A two-step approach can be used to site-specifically modify the 5'-cap of eukaryotic mRNAs. First, a trimethylguanosinesynthase variant recognizes the m(7)G cap structure and introduces bioorthogonal groups using S-adenosyl-L-methionine-based cosubstrates. Then, the enzymatically introduced reporter groups are further modified by thiol-ene or CuAAC click chemistry (see scheme).
authors.affiliation: Universität Hamburg, Department Chemie, Institut für Biochemie und Molekularbiologie, Martin-Luther-King Platz 6, 20146 Hamburg, Germany.
authors.forename: Daniela
authors.initials: D
authors.lastname: Schulz
authors.forename: Josephin Marie
authors.initials: JM
authors.lastname: Holstein
authors.forename: Andrea
authors.initials: A
authors.lastname: Rentmeister
mesh_terms: D057930:Click Chemistry; D006801:Humans; D008780:Methyltransferases / Q000378:metabolism*; D015394:Molecular Structure; D012315:RNA Caps / Q000737:chemistry* / Q000378:metabolism; D012333:RNA, Messenger / Q000737:chemistry* / Q000378:metabolism; D012436:S-Adenosylmethionine / Q000737:chemistry*; D013379:Substrate Specificity
chemical_list: D012315:RNA Caps; D012333:RNA, Messenger; D012436:S-Adenosylmethionine; D008780:Methyltransferases; C456271:trimethylguanosine synthase
keywords: AdoMet analogues; click chemistry; mRNA; trimethylguanosinesynthase