title: Carbodiimide crosslinked collagen from porcine dermal matrix for high-strength tissue engineering scaffold.
abstract: Naturally-derived collagens for tissue engineering are limited by low mechanical strength and rapid degradation. In this study, carbodiimide is used to chemically modify the collagen derived from porcine acellular dermal matrix (PADM). The results show that the strength and resistance of PADM to enzymatic digestion can be adjusted by the reconnection of free amino and carboxyl groups of the collagen fibers. The cytocompatibility of the crosslinked PADM was evaluated by cell adhesion and proliferation assays. The cell culture studies on crosslinked and uncrosslinked PADM showed that the modification does not affect the scaffold's biocompatibility. These results demonstrate that the PADM collagen materials can be strengthened through a low-cost, non-toxic crosslinking method for potential use in biomedical applications.
authors.affiliation: Center of Bio & Micro/nano Functional Materials, State Key Laboratory of Crystal Materials, Shandong University, 27 Shanda Nanlu, Jinan 250100, PR China.
authors.forename: Jianhua
authors.initials: J
authors.lastname: Li
authors.forename: Na
authors.initials: N
authors.lastname: Ren
authors.forename: Jichuan
authors.initials: J
authors.lastname: Qiu
authors.forename: Huaidong
authors.initials: H
authors.lastname: Jiang
authors.forename: Hongshi
authors.initials: H
authors.lastname: Zhao
authors.forename: Guancong
authors.initials: G
authors.lastname: Wang
authors.forename: Robert I
authors.initials: RI
authors.lastname: Boughton
authors.forename: Yingjun
authors.initials: Y
authors.lastname: Wang
authors.forename: Hong
authors.initials: H
authors.lastname: Liu
mesh_terms: D062088:Acellular Dermis; D000818:Animals; D001672:Biocompatible Materials; D002234:Carbodiimides / Q000737:chemistry*; D002460:Cell Line; D003094:Collagen / Q000737:chemistry*; D005109:Extracellular Matrix / Q000737:chemistry*; D008422:Materials Testing; D051379:Mice; D017550:Spectroscopy, Fourier Transform Infrared; D013552:Swine; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D002234:Carbodiimides; D003094:Collagen
keywords: Carbodiimide; Crosslinking; Cytocompatibility; Degradation; Mechanical properties; Porcine dermal collagen