title: RGD-conjugated iron oxide magnetic nanoparticles for magnetic resonance imaging contrast enhancement and hyperthermia.
abstract: The purpose of this study was to develop a specific targeting magnetic nanoparticle probe for magnetic resonance imaging and therapy in the form of local hyperthermia. Carboxymethyl dextran-coated ultrasmall superparamagnetic iron oxide nanoparticles with carboxyl groups were coupled to cyclic arginine-glycine-aspartic peptides for integrin α(v)β₃ targeting. The particle size, magnetic properties, heating effect, and stability of the arginine-glycine-aspartic-ultrasmall superparamagnetic iron oxide were measured. The arginine-glycine-aspartic-ultrasmall superparamagnetic iron oxide demonstrates excellent stability and fast magneto-temperature response. Magnetic resonance imaging signal intensity of Bcap37 cells incubated with arginine-glycine-aspartic-ultrasmall superparamagnetic iron oxide was significantly decreased compared with that incubated with plain ultrasmall superparamagnetic iron oxide. The preferential uptake of arginine-glycine-aspartic-ultrasmall superparamagnetic iron oxide by target cells was further confirmed by Prussian blue staining and confocal laser scanning microscopy.
authors.affiliation: 1College of Chemistry, Chemical Engineering and Materials Science & Key Laboratory of Organic Synthesis of Jiangsu Province, Soochow University, SIP, Suzhou, China.
authors.forename: S W
authors.initials: SW
authors.lastname: Zheng
authors.forename: M
authors.initials: M
authors.lastname: Huang
authors.forename: R Y
authors.initials: RY
authors.lastname: Hong
authors.forename: S M
authors.initials: SM
authors.lastname: Deng
authors.forename: L F
authors.initials: LF
authors.lastname: Cheng
authors.forename: B
authors.initials: B
authors.lastname: Gao
authors.forename: D
authors.initials: D
authors.lastname: Badami
mesh_terms: D002460:Cell Line; D045744:Cell Line, Tumor; D003287:Contrast Media; D005290:Ferric Compounds / Q000737:chemistry*; D005334:Fever; D006801:Humans; D008279:Magnetic Resonance Imaging / Q000379:methods*; D008280:Magnetics; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles; D009842:Oligopeptides / Q000737:chemistry*; D000091402:Prohibitins
chemical_list: D003287:Contrast Media; D005290:Ferric Compounds; D009842:Oligopeptides; C000717331:PHB2 protein, human; D000091402:Prohibitins; C000499:ferric oxide; C047981:arginyl-glycyl-aspartic acid
keywords: Magnetic nanoparticle; arginine-glycine-aspartic peptide; hyperthermia; magnetic resonance imaging; specific targeting