title: Fabrication of a polyaniline ultramicroelectrode via a self assembled monolayer modified gold electrode.
abstract: Herein, we report a simple and inexpensive way for the fabrication of an ultramicroelectrode and present its characterization by electrochemical techniques. The fabrication of polyaniline UME involves only two steps: modification of a gold (Au) electrode by self assembled monolayers (SAM) and then electrodeposition of polyaniline film on this thiol-coated Au electrode by using cyclic voltammetry and constant potential electrolysis methods. Two types of self-assembled monolayers (4-mercapto-1-butanol, MB, and 11-mercaptoundecanoic acid, MUA) were used, respectively, to see the effect of chain length on microelectrode formation. Microelectrode fabrication and utility of the surface was investigated by cyclic voltammetric measurements in a redox probe. The thus prepared polyaniline microelectrode was then used for DNA immobilization. Discrimination between double-stranded DNA (dsDNA) and single-stranded DNA (ssDNA) was obtained with enhanced electrochemical signals compared to a polyaniline-coated Au electrode. Different modifications on the electrode surfaces were examined using scanning electron microscopy (SEM).
authors.affiliation: Department of Chemistry, Faculty of Science, Hacettepe University, Beytepe-Ankara 06800, Turkey. gbolat@hacettepe.edu.tr
authors.forename: Gulcin
authors.initials: G
authors.lastname: Bolat
authors.forename: Filiz
authors.initials: F
authors.lastname: Kuralay
authors.forename: Gunes
authors.initials: G
authors.lastname: Eroglu
authors.forename: Serdar
authors.initials: S
authors.lastname: Abaci
mesh_terms: D000814:Aniline Compounds / Q000737:chemistry*; D015374:Biosensing Techniques; D003217:Conductometry / Q000295:instrumentation*; D004247:DNA / Q000032:analysis / Q000737:chemistry / Q000235:genetics*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006046:Gold / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry; D008839:Microelectrodes; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation*
chemical_list: D000814:Aniline Compounds; C416807:polyaniline; D006046:Gold; D004247:DNA