title: In vitro antitumor properties of an isolate from leaves of Cassia alata L.
abstract: Leaf extracts of Cassia alata L (akapulko), traditionally used for treatment of a variety of diseases, were evaluated for their potential antitumor properties in vitro. MTT assays were used to examine the cytotoxic effects of crude extracts on five human cancer cell lines, namely MCF-7, derived from a breast carcinoma, SK-BR-3, another breast carcinoma, T24 a bladder carcinoma, Col 2, a colorectal carcinoma, and A549, a non- small cell lung adenocarcinoma. Hexane extracts showed remarkable cytotoxicity against MCF-7, T24, and Col 2 in a dose-dependent manner. This observation was confirmed by morphological investigation using light microscopy. Further bioassay-directed fractionation of the cytotoxic extract led to the isolation of a TLC-pure isolate labeled as f6l. Isolate f6l was further evaluated using MTT assay and morphological and biochemical investigations, which likewise showed selectivity to MCF-7, T24, and Col 2 cells with IC50 values of 16, 17, and 17 μg/ml, respectively. Isolate f6l, however, showed no cytotoxicity towards the non-cancer Chinese hamster ovarian cell line (CHO-AA8). Cytochemical investigation using DAPI staining and biochemical investigation using terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL)-a method used to detect DNA fragmentation-together with caspase assay, demonstrated apoptotic cell death. Spectral characterization of isolate f6l revealed that it contained polyunsaturated fatty acid esters. Considering the cytotoxicity profile and its mode of action, f6l might represent a new promising compound with potential for development as an anticancer drug with low or no toxicity to non-cancer cells used in this study.
authors.affiliation: Institute of Biology, College of Science, University of the Philippines, Diliman, Quezon City, Philippines. liziglesias.olarte@gmail.com
authors.forename: Elizabeth Iglesias
authors.initials: EI
authors.lastname: Olarte
authors.forename: Annabelle Aliga
authors.initials: AA
authors.lastname: Herrera
authors.forename: Irene Manese
authors.initials: IM
authors.lastname: Villaseñor
authors.forename: Sonia Donaldo
authors.initials: SD
authors.lastname: Jacinto
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D016466:CHO Cells; D053148:Caspase 3 / Q000378:metabolism; D002366:Cassia / Q000737:chemistry*; D049109:Cell Proliferation / Q000187:drug effects*; D006224:Cricetinae; D003412:Cricetulus; D005434:Flow Cytometry; D006801:Humans; D066298:In Vitro Techniques; D009369:Neoplasms / Q000188:drug therapy* / Q000201:enzymology / Q000473:pathology; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry*; D014407:Tumor Cells, Cultured
chemical_list: D010936:Plant Extracts; D053148:Caspase 3