title: Komagataella kurtzmanii sp. nov., a new sibling species of Komagataella (Pichia) pastoris based on multigene sequence analysis.
abstract: A novel methanol assimilating yeast species Komagataella kurtzmanii is described using the type strain VKPM Y-727 (=KBP Y-2878 = UCD-FST 76-20 = Starmer #75-208.2 = CBS 12817 = NRRL Y-63667) isolated by W.T. Starmer from a fir flux in the Catalina Mountains, Southern AZ, USA. The new species is registered in MycoBank under MB 803919. The species was differentiated by divergence in gene sequences for D1/D2 LSU rRNA, ITS1-5.8S-ITS2, RNA polymerase subunit I, translation elongation factor-1α and mitochondrial small subunit rRNA. K. kurtzmanii differs from its phenotypically similar sibling species Komagataella pastoris, Komagataella pseudopastoris, Komagataella phaffii, Komagataella populi and Komagataella ulmi by absence of growth at 35 °C and inability to assimilate trehalose.
authors.affiliation: State Institute for Genetics and Selection of Industrial Microorganisms, 1-Dorozhnyi 1, Moscow, Russia. gnaumov@yahoo.com
authors.forename: Gennadi I
authors.initials: GI
authors.lastname: Naumov
authors.forename: Elena S
authors.initials: ES
authors.lastname: Naumova
authors.forename: Oleg V
authors.initials: OV
authors.lastname: Tyurin
authors.forename: Dmitry G
authors.initials: DG
authors.lastname: Kozlov
mesh_terms: D001130:Arizona; D016000:Cluster Analysis; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D004272:DNA, Mitochondrial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics; D012321:DNA-Directed RNA Polymerases / Q000235:genetics; D004783:Environmental Microbiology; D020459:Genes, rRNA; D000432:Methanol / Q000378:metabolism; D008853:Microscopy; D008969:Molecular Sequence Data; D016533:Mycological Typing Techniques; D020648:Peptide Elongation Factor 1 / Q000235:genetics; D010802:Phylogeny; D012331:RNA, Fungal / Q000235:genetics; D012335:RNA, Ribosomal / Q000235:genetics; D012340:RNA, Ribosomal, 5.8S / Q000235:genetics; D004718:Saccharomycetales / Q000145:classification* / Q000235:genetics* / Q000378:metabolism; D017422:Sequence Analysis, DNA
chemical_list: D004271:DNA, Fungal; D004272:DNA, Mitochondrial; D004275:DNA, Ribosomal; D021903:DNA, Ribosomal Spacer; D020648:Peptide Elongation Factor 1; D012331:RNA, Fungal; D012335:RNA, Ribosomal; D012340:RNA, Ribosomal, 5.8S; C065096:RNA, ribosomal, 26S; D012321:DNA-Directed RNA Polymerases; D000432:Methanol