title: Functionalization of iron oxide nanoparticles with biosurfactants and biocompatibility studies.
abstract: We present methodologies to functionalize iron oxide (Fe3O4) nanoparticles with biosurfactants and biocompatibility results. Positively charged Fe3O4 nanoparticles of average hydrodynamic size -26 nm is functionalized with four different molecules of interest, viz., surfactin, rhamnolipid, polyethylene glycol (PEG) and dextran. The functionalization results in dramatic alterations in surface potential and hydrodynamic size due to the presence of coated moieties on the nanoparticle interface. The Fourier transform infrared spectroscopy and thermogravimetric analysis confirm the presence of adsorbed moieties on nanoparticles. The phase contrast microscopy studies show the formation of reversible chains of functionalized nanoparticles under an external magnetic field. Cell viability studies using L929 mouse fibroblast cell line show that pure surfactin, rhamnolipid and dextran exhibit cytotoxicity with increase in concentration, whereas, pure PEG exhibit biocompatibility at different concentrations. Accordingly, surfactin and rhamnolipid coated nanoparticles are found to be cytotoxic with increase in concentration and PEG coated nanoparticles are found to be biocompatible. Dextran coated nanoparticles do not exhibit significant increase in biocompatibility.
authors.affiliation: SMARTS, Metallurgy and Materials Group, Indira Gandhi Centre for Atomic Research, Kalpakkam 603102, India.
authors.forename: J
authors.initials: J
authors.lastname: Sangeetha
authors.forename: Susha
authors.initials: S
authors.lastname: Thomas
authors.forename: J
authors.initials: J
authors.lastname: Arutchelvi
authors.forename: Mukesh
authors.initials: M
authors.lastname: Doble
authors.forename: John
authors.initials: J
authors.lastname: Philip
mesh_terms: D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000737:chemistry / Q000494:pharmacology*; D016503:Drug Delivery Systems; D004353:Drug Evaluation, Preclinical; D005290:Ferric Compounds / Q000737:chemistry* / Q000494:pharmacology; D005347:Fibroblasts / Q000187:drug effects / Q000502:physiology; D057446:Hydrodynamics; D008422:Materials Testing; D051379:Mice; D008954:Models, Biological; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D013501:Surface-Active Agents / Q000008:administration & dosage* / Q000494:pharmacology; D013818:Thermogravimetry
chemical_list: D020099:Coated Materials, Biocompatible; D005290:Ferric Compounds; D013501:Surface-Active Agents; C000499:ferric oxide