title: A tosyl-activated magnetic bead cellulose as solid support for sensitive protein detection.
abstract: Magnetic bead cellulose (MBC) was prepared using sol-gel transition of viscose in the presence of maghemite (γ-Fe₂O₃) nanoparticles. The MBC particles were then activated with p-toluenesulfonyl chloride to yield tosyl-activated magnetic bead cellulose (MBC-Ts). The microspheres were characterized by light and electron microscopy, elemental analysis and atomic absorption spectroscopy to determine morphology, size, polydispersity and content of iron and tosyl groups. The functionality of the MBC-Ts microspheres was demonstrated using proximity ligation assay (PLA) to detect vascular endothelial growth factor in femtomolar concentration range. The MBC-Ts microspheres performed equally well as commercially available microparticles that are routinely used as solid support in solid phase PLA.
authors.affiliation: Department of Immunology, Genetics and Pathology, Science for Life Laboratory, Uppsala University, Rudbecklaboratoriet, 75185 Uppsala, Sweden.
authors.forename: Junhong
authors.initials: J
authors.lastname: Yan
authors.forename: Daniel
authors.initials: D
authors.lastname: Horák
authors.forename: Jiří
authors.initials: J
authors.lastname: Lenfeld
authors.forename: Maria
authors.initials: M
authors.lastname: Hammond
authors.forename: Masood
authors.initials: M
authors.lastname: Kamali-Moghaddam
mesh_terms: D000818:Animals; D002482:Cellulose / Q000737:chemistry*; D002645:Chickens; D007118:Immunoassay / Q000379:methods; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D008863:Microspheres; D008956:Models, Chemical; D011506:Proteins / Q000032:analysis*; D014105:Tosyl Compounds / Q000737:chemistry*; D042461:Vascular Endothelial Growth Factor A / Q000097:blood
chemical_list: D058185:Magnetite Nanoparticles; D011506:Proteins; D014105:Tosyl Compounds; D042461:Vascular Endothelial Growth Factor A; C025506:4-toluenesulfonyl chloride; D002482:Cellulose
keywords: Bead cellulose; Magnetic; Protein detection; Proximity ligation assay