title: p38γ overexpression in gliomas and its role in proliferation and apoptosis.
abstract: The objective of this study was to confirm the biological role of p38γ in human gliomas. The expression profiles of p38γ and hTERT in human glioma samples were detected by Western Blot and immunohistochemistry. RNA interference was performed in U251 cells by p38γ silencing. Cell proliferation and apoptosis were assayed by CCK-8 and flow cytometric analysis, and then RNA and protein expression levels were measured by real-time RT-PCR and Western Blot, respectively. Telomerase activity assays and Caspase-3,-9 activation assays were also conducted. The results showed p38γ had a positive correlation with the glioma's malignancy grade and that the treatment of U251 cells with p38γ-siRNA inhibited proliferation and induced apoptosis. Correspondingly, hTERT expression and telomerase activity were down regulated and Caspase-3 and -9 activities were elevated. In conclusion, p38γ may serve as an oncogenic factor promoting the growth and progression of gliomas and may become a useful therapeutic target.
authors.affiliation: Department of Neurosurgery, Xiangya Hospital, Central South University, Changsha 410008, China.
authors.forename: Kui
authors.initials: K
authors.lastname: Yang
authors.forename: Yunsheng
authors.initials: Y
authors.lastname: Liu
authors.forename: Zhixiong
authors.initials: Z
authors.lastname: Liu
authors.forename: Jinfang
authors.initials: J
authors.lastname: Liu
authors.forename: Xin
authors.initials: X
authors.lastname: Liu
authors.forename: Xin
authors.initials: X
authors.lastname: Chen
authors.forename: Chuntao
authors.initials: C
authors.lastname: Li
authors.forename: Yu
authors.initials: Y
authors.lastname: Zeng
mesh_terms: D017209:Apoptosis; D015153:Blotting, Western; D001932:Brain Neoplasms / Q000201:enzymology* / Q000473:pathology; D049109:Cell Proliferation; D005910:Glioma / Q000201:enzymology* / Q000473:pathology; D006801:Humans; D048291:Mitogen-Activated Protein Kinase 12 / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D048291:Mitogen-Activated Protein Kinase 12