title: Rapid differentiation of cryptic species within Cryptococcus gattii by a duplex PCR assay.
abstract: Cryptococcus gattii consists of four cryptic species, VGI, VGII, VGIII, and VGIV. Herein, a duplex PCR assay using two primer pairs targeting the vacuolar membrane gene and the intergenic spacer region was developed. It successfully distinguished the cryptic species according to the distinct size of the amplicons.
authors.affiliation: Medical Mycology Laboratory, Department of Dermatology, Xinhua Hospital Affiliated to Shanghai Jiaotong University School of Medicine.
authors.forename: Xiaobo
authors.initials: X
authors.lastname: Feng
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Fu
authors.forename: Bo
authors.initials: B
authors.lastname: Ling
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.forename: Wanqing
authors.initials: W
authors.lastname: Liao
authors.forename: Weihua
authors.initials: W
authors.lastname: Pan
authors.forename: Zhirong
authors.initials: Z
authors.lastname: Yao
mesh_terms: D056285:Cryptococcus gattii / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D017931:DNA Primers / Q000235:genetics; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D021901:DNA, Intergenic; D005800:Genes, Fungal; D005838:Genotype; D006801:Humans; D008969:Molecular Sequence Data; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*; D017422:Sequence Analysis, DNA
chemical_list: D017931:DNA Primers; D004271:DNA, Fungal; D021901:DNA, Intergenic