title: MiR-424 regulates monocytic differentiation of human leukemia U937 cells by directly targeting CDX2.
abstract: MiR-424 plays an important role via promoting the monocytic differentiation in many human leukemia cell lines. Here, we report that miR-424 decreased miR-125b expression to 36 % by directly targeting caudal type homeobox 2. However, miR-424 also decreased expression of Fes, PU.1 and colony-stimulating factor receptor (MCSFR). As Fes, PU.1 and MCSFR were down-regulated by over-expression of miR-125b (unpublished work), a similar effect of miR-424 and Fes siRNA on CD64, Egr-1, Egr-2 and CEBPA indicates that Fes may be an important downstream target of miR-424. We hypothesize that miR-424 promotes monocytic differentiation by regulating other critical factors and miR-424 has high affinity for these factors. For the first time, the molecular mechanism of miR-424 during monocytic differentiation of U937 cells has been elucidated in this study.
authors.affiliation: School of Life Science and Technology, China Pharmaceutical University, Nanjing, 210009, People's Republic of China, shenxiaocpu@163.com.
authors.forename: Xiao
authors.initials: X
authors.lastname: Shen
authors.forename: Jinhai
authors.initials: J
authors.lastname: Tang
authors.forename: Jinhang
authors.initials: J
authors.lastname: Hu
authors.forename: Le
authors.initials: L
authors.lastname: Guo
authors.forename: Yingying
authors.initials: Y
authors.lastname: Xing
authors.forename: Tao
authors.initials: T
authors.lastname: Xi
mesh_terms: D000071616:CDX2 Transcription Factor; D002454:Cell Differentiation / Q000187:drug effects*; D015536:Down-Regulation; D015870:Gene Expression / Q000187:drug effects; D018398:Homeodomain Proteins / Q000378:metabolism*; D006801:Humans; D035683:MicroRNAs / Q000096:biosynthesis / Q000378:metabolism*; D009000:Monocytes / Q000187:drug effects* / Q000502:physiology; D011518:Proto-Oncogene Proteins / Q000096:biosynthesis; D051578:Proto-Oncogene Proteins c-fes / Q000096:biosynthesis; D016184:Receptors, Colony-Stimulating Factor / Q000096:biosynthesis; D015534:Trans-Activators / Q000096:biosynthesis; D020298:U937 Cells; D000099232:Proto-Oncogene Protein Spi-1
chemical_list: D000071616:CDX2 Transcription Factor; D018398:Homeodomain Proteins; D035683:MicroRNAs; D011518:Proto-Oncogene Proteins; D051578:Proto-Oncogene Proteins c-fes; D016184:Receptors, Colony-Stimulating Factor; D015534:Trans-Activators; D000099232:Proto-Oncogene Protein Spi-1; C491270:CDX2 protein, human; C531888:MIRN125 microRNA, human; C554214:MIRN424 microrna, human; C496645:FES protein, human