title: Hydrolysis of insoluble cellulose to glucose catalyzed by cellulase-containing liposomes in an aqueous solution of 1-butyl-3-methylimidazolium chloride.
abstract: The liposome containing cellulase from Trichoderma viride was prepared under the condition that an appreciable amount of cellulase was incorporated in lipid membranes. The liposomal cellulase and free enzyme were examined in their hydrolytic activities to insoluble cellulose powder CC31 in the acetate buffer solution (pH 4.8) of 15 w/w% [Bmim][Cl] (1-butyl-3-methylimidazolium chloride). The mean diameter and size distribution of cellulase-containing liposome were practically unchanged under the above condition. The free cellulase was deactivated more rapidly than the liposomal cellulase in catalyzing the hydrolysis of 2.0 g/l CC31 at 45°C in the presence of [Bmim][Cl] for 48 h. The activities of liposomal and free cellulase to cellobiose as soluble substrate were less susceptible to [Bmim][Cl] than their cellulolytic activities to CC31, meaning that β-glucosidase is relatively stable among the three enzyme components of cellulase. The rate of glucose production could be appreciably improved by the pretreatment of CC31 with [Bmim][Cl] alone at 120°C for 30 min followed by the liposomal cellulase-catalyzed hydrolysis of the substrate at 45°C at the [Bmim][Cl] concentration of 15 w/w%.
authors.affiliation: Dept. of Applied Molecular Bioscience, Yamaguchi University, 2-16-1 Tokiwadai, Ube, 755-8611, Japan.
authors.forename: Makoto
authors.initials: M
authors.lastname: Yoshimoto
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Tanimura
authors.forename: Kazuki
authors.initials: K
authors.lastname: Tokunaga
authors.forename: Akio
authors.initials: A
authors.lastname: Kamimura
mesh_terms: D002475:Cellobiose / Q000378:metabolism; D002480:Cellulase / Q000378:metabolism*; D002482:Cellulose / Q000737:chemistry*; D055598:Chemical Phenomena; D005947:Glucose / Q000737:chemistry*; D006868:Hydrolysis; D007093:Imidazoles / Q000737:chemistry*; D007218:Industrial Microbiology; D008081:Liposomes / Q000737:chemistry*; D014242:Trichoderma / Q000201:enzymology; D001617:beta-Glucosidase / Q000378:metabolism
chemical_list: D007093:Imidazoles; D008081:Liposomes; D002475:Cellobiose; C502841:1-butyl-3-methylimidazolium chloride; D002482:Cellulose; D001617:beta-Glucosidase; D002480:Cellulase; D005947:Glucose
keywords: cellulase; hydrolysis; insoluble cellulose; ionic liquid; liposomes