title: SYCP3-like X-linked 2 is expressed in meiotic germ cells and interacts with synaptonemal complex central element protein 2 and histone acetyltransferase TIP60.
abstract: Meiosis is the process by which diploid germ cells produce haploid gametes. A key event is the formation of the synaptonemal complex. In the pachytene stage, the unpaired regions of X and Y chromosomes form a specialized structure, the XY body, within which gene expression is mostly silenced. In the present study, we showed that SYCP3-like X-linked 2 (SLX2, 1700013H16Rik), a novel member of XLR (X-linked Lymphocyte-Regulated) family, was specifically expressed in meiotic germ cells. In the spermatocyte SLX2 was distributed in the nucleus of germ cells at the preleptotene, leptotene and zygotene stages and is then restricted to the XY body at the pachytene stage. This localization change is coincident with that of phosphorylated histone H2AX (γH2AX), a well-known component of the sex body. Through yeast two-hybrid screening and coimmunoprecipitation assays, we demonstrated that SLX2 interacts with synaptonemal complex central element protein 2 (SYCE2), an important component of synaptonemal complex, and histone acetyltransferase TIP60, which has been implicated in remodeling phospho-H2AX-containing nucleosomes at sites of DNA damage. These results suggest that SLX2 might be involved in DNA recombination, synaptonemal complex formation as well as sex body maintenance during meiosis.
authors.affiliation: State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, PR China.
authors.forename: Yu-Qiang
authors.initials: YQ
authors.lastname: Shi
authors.forename: Xin-Jie
authors.initials: XJ
authors.lastname: Zhuang
authors.forename: Bo
authors.initials: B
authors.lastname: Xu
authors.forename: Juan
authors.initials: J
authors.lastname: Hua
authors.forename: Shang-Ying
authors.initials: SY
authors.lastname: Liao
authors.forename: Qinghua
authors.initials: Q
authors.lastname: Shi
authors.forename: Howard J
authors.initials: HJ
authors.lastname: Cooke
authors.forename: Chunsheng
authors.initials: C
authors.lastname: Han
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D004260:DNA Repair; D015870:Gene Expression; D057809:HEK293 Cells; D051548:Histone Acetyltransferases / Q000378:metabolism*; D006657:Histones / Q000378:metabolism; D006801:Humans; D000074443:Lysine Acetyltransferase 5; D008297:Male; D008540:Meiosis; D051379:Mice; D009687:Nuclear Proteins / Q000235:genetics* / Q000378:metabolism*; D011485:Protein Binding; D025941:Protein Interaction Mapping; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D013090:Spermatocytes / Q000378:metabolism*; D013573:Synaptonemal Complex / Q000378:metabolism; D013737:Testis / Q000166:cytology; D015534:Trans-Activators / Q000378:metabolism*
chemical_list: D051548:Histone Acetyltransferases; D006657:Histones; D000074443:Lysine Acetyltransferase 5; D009687:Nuclear Proteins; D020033:Protein Isoforms; D015534:Trans-Activators; C504911:SYCE2 protein, mouse; C085792:Slx protein, mouse; C523065:Kat5 protein, mouse; C475961:gamma-H2AX protein, mouse
keywords: KAT/TIP60; Meiosis; SLX2; SYCE2; SYCP3; SYCP3-like X-linked 2; TIP60; X-linked lymphocyte regulated; Xlr; Xlr-related meiosis-regulated; Xmr; histone acetyltransferase Tip60; phosphorylated histone H2AX; synaptonemal complex central element protein 2; synaptonemal complex protein 3; γH2AX