title: [Effect of exendin-4 on monocyte chemoattractant protein-1 expression in cultured rat glomerular mesangial cells induced by tumor necrosis factor-α in vitro].
abstract: OBJECTIVE
  To investigate the effect of exendin-4 on the expression of monocyte chemoattractant protein-1 (MCP-1) and fibronectin (FN) in rat glomerular mesangial cells in vitro.
  
  
  METHODS
  Rat glomerular mesangial cells were divided into 5 groups, namely control group, tumor necrosis factor-α (TNF-α) group (10 ng/ml), TNF-α (10 ng/ml)+E1 (1 nmol/L exendin-4) group, TNF-α (10 ng/ml)+E5 (5 nmol/L exendin-4) group, and TNF-α (10 ng/ml)+E10 (10 nmol/L exendin-4) group. After cultured 24 h or 48 h, RNA were extracted to determine the expression of MCP-1 with real-time PCR, the supernatant were collected to determine the expression of MCP-1 and FN with ELISA.
  
  
  RESULTS
  Compared with control group, the cells treated with TNF-α for 24 h showed significantly increase the expression of MCP-1 and FN (P<0.01), exendin-4 significantly reduced the expression of MCP-1 and FN in TNF-α+E5 group and TNF-α+E10 group (P<0.05). After 48h incubation, the expression of MCP-1 and FN increased significantly in TNF-α group (P<0.01), which was lowered by exendin-4 in TNF-α+E1,TNF-α+E5 and TNF-α+E10 groups (P<0.05).
  
  
  CONCLUSIONS
  Exendin-4 has an intrinsic capability to concentration- and time-dependently inhibit TNF-α-induced expression of MCP-1 and FN in rat mesangial cells, suggesting the beneficial effect of exendin-4 in preventing and treating diabetic nephropathy.
authors.affiliation: Department of Endocrinology and Metabolism, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. yingjuanjiang@126.com
authors.forename: Yingjuan
authors.initials: Y
authors.lastname: Jiang
authors.forename: Yaoming
authors.initials: Y
authors.lastname: Xue
authors.forename: Qian
authors.initials: Q
authors.lastname: Zhang
authors.forename: Yanfei
authors.initials: Y
authors.lastname: Zhang
authors.forename: Yuan
authors.initials: Y
authors.lastname: Yuan
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D018932:Chemokine CCL2 / Q000378:metabolism*; D003928:Diabetic Nephropathies / Q000378:metabolism*; D000077270:Exenatide; D005920:Glomerular Mesangium / Q000166:cytology; D050527:Mesangial Cells / Q000187:drug effects / Q000378:metabolism*; D010455:Peptides / Q000494:pharmacology*; D051381:Rats; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology; D014688:Venoms / Q000494:pharmacology*
chemical_list: C491766:Ccl2 protein, rat; D018932:Chemokine CCL2; D010455:Peptides; D014409:Tumor Necrosis Factor-alpha; D014688:Venoms; D000077270:Exenatide