title: Synthesis of gold coated magnetic microparticles and their application for electrochemical glucose sensing by the enzymatically precipitated prussian blue.
abstract: An enzyme stimulated deposition of prussian blue onto the gold-coated magnetic microparticles is described. We propose to synthesize the continuous outer gold layer on the magnetic particle for a gold working electrode and its superparamagnetic property. In-depth characterization of the gold shell formation was studied with scanning electron microscopy, energy-dispersive X-ray spectroscopy, cyclic voltammetry. The gold-coated magnetic microparticles offered adhesive layer for the immobilization of glucose oxidase catalyzing the generation of prussian blue in the presence of glucose. The assembled prussian blue on the gold shell surfaces was detected with electrochemical measurements depending on the glucose concentration. With accomplishing the linear response range from 0.2 mM to 10 mM of glucose, this approach successfully proposed the applicability of the magnetic core-gold shell structures to the electrochemical bioassay area.
authors.affiliation: Molecular-Level Interface Research Center, Department of Chemistry, KAIST, Daejeon 305-701, Korea.
authors.forename: Hye Young
authors.initials: HY
authors.lastname: Jung
authors.forename: Jun Hui
authors.initials: JH
authors.lastname: Park
authors.forename: Seongpil
authors.initials: S
authors.lastname: Hwang
authors.forename: Juhyoun
authors.initials: J
authors.lastname: Kwak
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D002384:Catalysis; D011232:Chemical Precipitation; D020099:Coated Materials, Biocompatible / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D055664:Electrochemical Techniques / Q000295:instrumentation / Q000379:methods; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism; D005295:Ferrocyanides / Q000737:chemistry* / Q000493:pharmacokinetics; D005947:Glucose / Q000032:analysis* / Q000493:pharmacokinetics; D005949:Glucose Oxidase / Q000737:chemistry / Q000378:metabolism; D006046:Gold / Q000737:chemistry*; D058185:Magnetite Nanoparticles / Q000737:chemistry; D059346:Magnets / Q000737:chemistry*; D008863:Microspheres; D010316:Particle Size; D011540:Prussian Blue Reaction
chemical_list: D020099:Coated Materials, Biocompatible; D004800:Enzymes, Immobilized; D005295:Ferrocyanides; D058185:Magnetite Nanoparticles; D006046:Gold; D005949:Glucose Oxidase; D005947:Glucose; C000170:ferric ferrocyanide