title: Macromolecular juggling by ubiquitylation enzymes.
abstract: The posttranslational modification of target proteins with ubiquitin and ubiquitin-like proteins is accomplished by the sequential action of E1, E2, and E3 enzymes. Members of the E1 and E3 enzyme families can undergo particularly large conformational changes during their catalytic cycles, involving the remodeling of domain interfaces. This enables the efficient, directed and regulated handover of ubiquitin from one carrier to the next one. We review some of these conformational transformations, as revealed by crystallographic studies.
authors.affiliation: Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA.
authors.forename: Sonja
authors.initials: S
authors.lastname: Lorenz
authors.forename: Aaron J
authors.initials: AJ
authors.lastname: Cantor
authors.forename: Michael
authors.initials: M
authors.lastname: Rape
authors.forename: John
authors.initials: J
authors.lastname: Kuriyan
mesh_terms: D000818:Animals; D055162:Biocatalysis; D006801:Humans; D046911:Macromolecular Substances / Q000378:metabolism*; D010766:Phosphorylation; D017434:Protein Structure, Tertiary; D043743:Ubiquitin-Protein Ligase Complexes / Q000737:chemistry / Q000378:metabolism*; D054875:Ubiquitination
chemical_list: D046911:Macromolecular Substances; D043743:Ubiquitin-Protein Ligase Complexes