title: Short communication: Survey of animal-borne pathogens in the farm environment of 13 dairy operations.
abstract: A survey was conducted on 13 dairies to determine the occurrence of 5 animal-borne pathogens (Salmonella enterica, Escherichia coli O157:H7, Campylobacter jejuni, Mycobacterium avium ssp. paratuberculosis, and Cryptosporidium parvum) and their distributions across farm elements (feces, bedding, milk filters, stored manure, field soil, and stream water). Presence of C. parvum was measured only in feces and stored manure. All but one farm were positive for at least one pathogen species, and 5 farms were positive for 3 species. Escherichia coli O157:H7 was detected on 6 farms and in all farm elements, including milk filters. Mycobacterium avium ssp. paratuberculosis was detected on 10 of 13 farms and in all farm elements except for milk filters. Salmonella enterica and C. jejuni were detected at lower frequencies and were not identified in soil, stream water, or milk filters on any of the 13 farms. Cryptosporidium parvum was detected in feces but not in stored manure. Stored manure had the highest occurrence of pathogens (73%), followed by feces (50%), milk filters, bedding, soil, and water (range from 23 to 31%). Association of pathogen presence with farm management factors was examined by t-test; however, the small number of study farms and samples may limit the scope of inference of the associations. Pathogens had a higher prevalence in maternity pen bedding than in calf bedding, but total pathogen occurrence did not differ in calf compared with lactating cow feces or in soils with or without manure incorporation. Herd size and animal density did not appear to have a consistent effect on pathogen occurrence. The extent of pathogen prevalence and distribution on the farms indicates considerable public health risks associated with not only milk and meat consumption and direct animal contact, but also potential dissemination of the pathogens into the agroecosystem.
authors.affiliation: Department of Clinical Studies, School of Veterinary Medicine, University of Pennsylvania, Pennsylvania 19348, USA. jdtoth@vet.upenn.edu
authors.forename: J D
authors.initials: JD
authors.lastname: Toth
authors.forename: H W
authors.initials: HW
authors.lastname: Aceto
authors.forename: S C
authors.initials: SC
authors.lastname: Rankin
authors.forename: Z
authors.initials: Z
authors.lastname: Dou
mesh_terms: D000818:Animals; D016123:Campylobacter jejuni; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology*; D016785:Cryptosporidium parvum; D003612:Dairying / Q000706:statistics & numerical data*; D004197:Disease Reservoirs / Q000382:microbiology; D004777:Environment; D019453:Escherichia coli O157; D005243:Feces / Q000382:microbiology; D008372:Manure / Q000382:microbiology; D016927:Mycobacterium avium subsp. paratuberculosis; D019779:Salmonella enterica; D012988:Soil Microbiology; D014871:Water Microbiology
chemical_list: D008372:Manure
keywords: Escherichia coli O157:H7; Mycobacterium avium ssp. paratuberculosis; animal-borne pathogen; dairy environment