title: Breast epithelial cell infiltration in enhanced electrospun silk scaffolds.
abstract: In the present study, the effects of air-flow impedance electrospinning and air-flow rates on silk-based scaffolds for biological tissues were investigated. First, the properties of scaffolds obtained from 7% and 12% silk concentrations were defined. In addition, cell infiltration and viability of MCF-10A breast epithelial cells cultured onto these scaffolds were used to determine the biological suitability of these nanostructures. Air-flow impedance electrospun scaffolds resulted in an overall larger pore size than scaffolds electrospun on a solid mandrel, with the largest pores in 7% silk electrospun with an air pressure of 100 kPa and in 12% silk electrospun with an air pressure of 400 kPa (13.4 ± 0.67 and 26.03 ± 1.19 µm, respectively). After 14 days in culture, the deepest MCF-10A cell infiltration (36.58 ± 2.28 µm) was observed into 7% silk air-flow impedance electrospun scaffolds subjected to an air pressure of 100 kPa. In those scaffolds MCF-10A cell viability was also highest after 14 days in culture. Together, these results strongly support the use of 7% silk-based scaffolds electrospun with a 100 kPa air-flow as the most suitable microenvironment for MCF-10A infiltration and viability.
authors.affiliation: Department of Biomedical Engineering, Virginia Commonwealth University, Richmond, VA, USA.|Department of Biology, University of North Carolina at Charlotte, Charlotte, NC, USA.
authors.forename: Yas
authors.initials: Y
authors.lastname: Maghdouri-White
authors.affiliation: Department of Pathology, Virginia Commonwealth University, Richmond, VA, USA.|Massey Cancer Center, Virginia Commonwealth University, Richmond, VA, USA.
authors.forename: Lynne W
authors.initials: LW
authors.lastname: Elmore
authors.affiliation: Department of Biomedical Engineering, Virginia Commonwealth University, Richmond, VA, USA.
authors.forename: Gary L
authors.initials: GL
authors.lastname: Bowlin
authors.affiliation: Department of Biology, University of North Carolina at Charlotte, Charlotte, NC, USA.
authors.forename: Didier
authors.initials: D
authors.lastname: Dréau
mesh_terms: D000388:Air; D000818:Animals; D012831:Bombyx; D001940:Breast / Q000166:cytology*; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004847:Epithelial Cells / Q000166:cytology* / Q000187:drug effects; D005260:Female; D006801:Humans; D008855:Microscopy, Electron, Scanning; D016062:Porosity; D047011:Silk / Q000737:chemistry* / Q000494:pharmacology; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D047011:Silk
keywords: breast tissue engineering; electrospinning; epithelial cells; porosity; scaffold; silk