title: Metabolic potential of the organic-solvent tolerant Pseudomonas putida DOT-T1E deduced from its annotated genome.
abstract: Pseudomonas putida DOT-T1E is an organic solvent tolerant strain capable of degrading aromatic hydrocarbons. Here we report the DOT-T1E genomic sequence (6,394,153 bp) and its metabolic atlas based on the classification of enzyme activities. The genome encodes for at least 1751 enzymatic reactions that account for the known pattern of C, N, P and S utilization by this strain. Based on the potential of this strain to thrive in the presence of organic solvents and the subclasses of enzymes encoded in the genome, its metabolic map can be drawn and a number of potential biotransformation reactions can be deduced. This information may prove useful for adapting desired reactions to create value-added products. This bioengineering potential may be realized via direct transformation of substrates, or may require genetic engineering to block an existing pathway, or to re-organize operons and genes, as well as possibly requiring the recruitment of enzymes from other sources to achieve the desired transformation.
authors.affiliation: Estación Experimental del Zadín-CSIC, Profesor Albareda 1, 18008 Granada, Spain.
authors.forename: Zulema
authors.initials: Z
authors.lastname: Udaondo
authors.forename: Lazaro
authors.initials: L
authors.lastname: Molina
authors.forename: Craig
authors.initials: C
authors.lastname: Daniels
authors.forename: Manuel J
authors.initials: MJ
authors.lastname: Gómez
authors.forename: María A
authors.initials: MA
authors.lastname: Molina-Henares
authors.forename: Miguel A
authors.initials: MA
authors.lastname: Matilla
authors.forename: Amalia
authors.initials: A
authors.lastname: Roca
authors.forename: Matilde
authors.initials: M
authors.lastname: Fernández
authors.forename: Estrella
authors.initials: E
authors.lastname: Duque
authors.forename: Ana
authors.initials: A
authors.lastname: Segura
authors.forename: Juan Luis
authors.initials: JL
authors.lastname: Ramos
mesh_terms: D001711:Biotransformation; D004269:DNA, Bacterial / Q000737:chemistry* / Q000235:genetics*; D016680:Genome, Bacterial; D053858:Metabolic Networks and Pathways / Q000235:genetics*; D008969:Molecular Sequence Data; D016958:Pseudomonas putida / Q000235:genetics* / Q000378:metabolism*; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial