title: 3-O-Acetyloleanolic acid exhibits anti-angiogenic effects and induces apoptosis in human umbilical vein endothelial cells.
abstract: 3-O-Acetyloleanolic acid, a pentacyclic triterpenoid isolated from cowpea seeds, inhibited proliferation, migration and tube formation of human umbilical vein endothelial cells (HUVECs) in a dose-dependent manner. HUVECs. The induced apoptosis was characterized by detection of cell surface annexin V and sub-G1 populations. The number of cells immunostained with annexin V-fluorescein isothiocyanate increased after treatment with 3-O-acetyloleanolic acid. The sub-G1 cell populations were also increased in treated HUVECs. 3-O-Acetyloleanolic acid induced activation of caspase 3, a critical mediator of apoptosis signaling. It also significantly inhibited angiogenesis in an in vivo Matrigel plug assay. 3-O-Acetyloleanolic acid thus exhibits anti-angiogenic effects and induces apoptosis in HUVECs and the results suggest that it has a potential use for suppression of the tumor growth stimulated by angiogenesis.
authors.affiliation: Department of Genetic Engineering, Kyung Hee University, Yongin, 446-701, Korea.
authors.forename: En-Ji
authors.initials: EJ
authors.lastname: Cui
authors.forename: Jeon
authors.initials: J
authors.lastname: Hwang-Bo
authors.forename: Jong-Hwa
authors.initials: JH
authors.lastname: Park
authors.forename: Nam-In
authors.initials: NI
authors.lastname: Baek
authors.forename: Jiyoung
authors.initials: J
authors.lastname: Kim
authors.forename: Seong Gil
authors.initials: SG
authors.lastname: Hong
authors.forename: In Sik
authors.initials: IS
authors.lastname: Chung
mesh_terms: D020533:Angiogenesis Inhibitors / Q000302:isolation & purification / Q000378:metabolism*; D017209:Apoptosis; D007887:Fabaceae / Q000737:chemistry; D061307:Human Umbilical Vein Endothelial Cells / Q000187:drug effects*; D006801:Humans; D012639:Seeds / Q000737:chemistry; D014315:Triterpenes / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D020533:Angiogenesis Inhibitors; D014315:Triterpenes; C052658:oleanolic acid 3-acetate