title: Sequential addition of reprogramming factors improves efficiency.
abstract: Addition of a specific set of transcription factors reprograms somatic cell nuclei to a pluripotent state. Sequential addition of these factors, rather than the simultaneous exposure used in standard protocols, improves reprogramming efficiency. This sequential method favours a transition through a state with enhanced mesenchymal characteristics before driving an epithelial transformation on the way to the pluripotent state.
authors.forename: X
authors.initials: X
authors.lastname: Gaeta
authors.forename: Y
authors.initials: Y
authors.lastname: Xie
authors.forename: W E
authors.initials: WE
authors.lastname: Lowry
mesh_terms: D000818:Animals; D065150:Cellular Reprogramming; D058750:Epithelial-Mesenchymal Transition; D057026:Induced Pluripotent Stem Cells / Q000473:pathology*; D000090062:Kruppel-Like Factor 4; D051741:Kruppel-Like Transcription Factors / Q000378:metabolism*; D050814:Octamer Transcription Factor-3 / Q000378:metabolism*; D016271:Proto-Oncogene Proteins c-myc / Q000378:metabolism*; D055748:SOXB1 Transcription Factors / Q000378:metabolism*
chemical_list: D000090062:Kruppel-Like Factor 4; D051741:Kruppel-Like Transcription Factors; D050814:Octamer Transcription Factor-3; C494174:Pou5f1 protein, mouse; D016271:Proto-Oncogene Proteins c-myc; D055748:SOXB1 Transcription Factors; C527783:Sox2 protein, mouse