title: A serine proteinase PmClipSP2 contributes to prophenoloxidase system and plays a protective role in shrimp defense by scavenging lipopolysaccharide.
abstract: Serine proteinases (SPs) participate in various biological processes and play vital role in immunity. In this study, we investigated the function of PmClipSP2 from shrimp Penaeus monodon in defense against bacterial infection. PmClipSP2 was identified as a clip-domain SP and its mRNA increased in response to infection with Vibrio harveyi. PmClipSP2-knockdown shrimp displayed a significantly reduced phenoloxidase (PO) activity and increased susceptibility to V. harveyi infection. Injection of LPS and/or β-1,3-glucan induced a dose-dependent mortality and a significant decrease in the number of total hemocytes, with clear morphological changes in the hemocyte surface, of the PmClipSP2-knockdown shrimp. Recombinant PmClipSP2 was shown to bind to LPS and β-1,3-glucan and to activate PO activity. These results reveal that PmClipSP2 acts as a pattern-recognition protein, binding to microbial polysaccharides and likely activating the proPO system, whilst it may play an essential role in the hemocyte homeostasis by scavenging LPS and neutralizing its toxicity.
authors.affiliation: Center of Excellence for Molecular Biology and Genomics of Shrimp, Department of Biochemistry, Faculty of Science, Chulalongkorn University, 254 Phayathai Road, Bangkok 10330, Thailand; National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), 113 Paholyothin Road, Klong1, Klong Luang, Pathum Thani 12120, Thailand.
authors.forename: Piti
authors.initials: P
authors.lastname: Amparyup
authors.forename: Kanyanat
authors.initials: K
authors.lastname: Promrungreang
authors.forename: Walaiporn
authors.initials: W
authors.lastname: Charoensapsri
authors.forename: Jantiwan
authors.initials: J
authors.lastname: Sutthangkul
authors.forename: Anchalee
authors.initials: A
authors.lastname: Tassanakajon
mesh_terms: D000818:Animals; D004156:Catechol Oxidase / Q000235:genetics / Q000276:immunology*; D003445:Crustacea / Q000235:genetics* / Q000276:immunology*; D004792:Enzyme Precursors / Q000235:genetics / Q000276:immunology*; D006434:Hemocytes / Q000276:immunology; D008070:Lipopolysaccharides / Q000276:immunology*; D051192:Receptors, Pattern Recognition / Q000235:genetics / Q000276:immunology; D057057:Serine Proteases / Q000235:genetics / Q000276:immunology*; D014733:Vibrio / Q000276:immunology; D014735:Vibrio Infections / Q000276:immunology; D047071:beta-Glucans / Q000276:immunology
chemical_list: D004792:Enzyme Precursors; D008070:Lipopolysaccharides; D051192:Receptors, Pattern Recognition; D047071:beta-Glucans; C033363:beta-1,3-glucan; C038451:pro-phenoloxidase; D004156:Catechol Oxidase; D057057:Serine Proteases
keywords: Hemocyte homeostasis; Pattern recognition protein; Penaeus monodon; Prophenoloxidase; Serine proteinase; Shrimp immunity