title: Production and characterization of humanized Rag2-/-γc -/- mice.
abstract: Mice reconstituted with human immune cells represent a model to study the development and functionality of the human immune system. Recent improvements in humanized mice have resulted in multi-lineage hematopoiesis, prolonged human cell engraftment that is detectable in many mouse organs, and the ability to generate de novo human innate and adaptive immune responses. Here, we describe the methods used to produce and characterize humanized Rag2(-/-)γc(-/-) mice.
authors.affiliation: Department of Microbiology and Molecular Biology, Brigham Young University, Provo, UT, USA.
authors.forename: Freddy M
authors.initials: FM
authors.lastname: Sanchez
authors.forename: German I
authors.initials: GI
authors.lastname: Cuadra
authors.forename: Stanton J
authors.initials: SJ
authors.lastname: Nielsen
authors.forename: Anne
authors.initials: A
authors.lastname: Tanner
authors.forename: Bradford K
authors.initials: BK
authors.lastname: Berges
mesh_terms: D000818:Animals; D061067:Antibodies, Monoclonal, Humanized; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000276:immunology; D004195:Disease Models, Animal; D006410:Hematopoiesis / Q000276:immunology; D006801:Humans; D007107:Immune System; D051379:Mice; D008822:Mice, Transgenic / Q000235:genetics*; D009687:Nuclear Proteins / Q000235:genetics* / Q000276:immunology; D014183:Transplantation, Heterologous / Q000379:methods*
chemical_list: D061067:Antibodies, Monoclonal, Humanized; D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; C499311:RAG2 protein, human