title: Characterization and quantification of 10-hydroxycamptothecine in Camptotheca acuminate and its medicinal preparation by liquid chromatography-ion trap mass spectrometry.
abstract: A rapid and sensitive method for the identification and quantification of 10-hydroxycamptothecine (HCPT) in Camptotheca acuminata Decne is described. The HCPT standard solution was directly infused into the ion trap mass spectrometers (IT/MS) for collecting the MS(n) spectra. The electrospray ionization (ESI) mass spectral fragmentation pathway of HCPT was proposed and the ESI-MS(n) fragmentation behavior of HCPT was deduced in detail. The major fragment ions of HCPT were confirmed by MS(n) in both negative ion and positive ion mode. The possible main cleavage pathway of fragment ions was studied. Quantification of HCPT was assigned in negative-ion mode at a product ion at m/z 363 → 319 by LC-MS. The LC-MS method was validated for linearity, sensitivity, accuracy and precision, and then used to determine the content of the HCPT. Lastly, the LC-MS method was successfully applied to determine HCPT in real samples of Camptotheca acuminate Decne and its medicinal preparation in the first time.
authors.affiliation: Institute of Pharmaceutical Analysis and Drug Screening, Affiliated Dongfeng Hospital, Hubei University of Medicine, Hubei, 442008, China.
authors.forename: Qinhua
authors.initials: Q
authors.lastname: Chen
authors.forename: Guangmin
authors.initials: G
authors.lastname: Pan
authors.forename: Lin
authors.initials: L
authors.lastname: Xiong
authors.forename: Hongsheng
authors.initials: H
authors.lastname: He
authors.forename: Hangdong
authors.initials: H
authors.lastname: Yang
mesh_terms: D029769:Camptotheca / Q000737:chemistry*; D002166:Camptothecin / Q000031:analogs & derivatives* / Q000032:analysis / Q000737:chemistry; D002853:Chromatography, Liquid / Q000379:methods*; D005638:Fruit / Q000737:chemistry; D007477:Ions / Q000737:chemistry; D013058:Mass Spectrometry / Q000379:methods*; D010936:Plant Extracts / Q000737:chemistry
chemical_list: D007477:Ions; D010936:Plant Extracts; C028098:10-hydroxycamptothecin; D002166:Camptothecin
keywords: 10-hydroxycamptothecine; Camptotheca acuminate Decne; fragmentation pathways; liquid chromatography-ion trap mass spectrometry