title: The altered expression profile of microRNAs in cardiopulmonary bypass canine models and the effects of mir-499 on myocardial ischemic reperfusion injury.
abstract: BACKGROUND
  MicroRNAs were enrolled in various cardiovascular disease especially ischemic heart diseases, but the microRNA changes during myocardial ischemia reperfusion injury underwent cardiopulmonary bypass are still unknown. This study screens the microRNA differences in CPB canines and evaluates the relationship of microRNAs with myocardial ischemia reperfusion injury.
  
  
  METHODS
  13 healthy canines received CPB with 60 minutes of aortic clamping and cardioplegic arrest, followed by 90 minutes reperfusion. Left ventricular myocardial samples, blood samples and hemodynamic data were taken at different time points. We performed microRNAs microarray experiments upon the left ventricle myocardium tissue of canines before CPB and after reperfusion for 90 minutes by pooling 3 tissue samples together and used qRT-PCR for confirmation.
  
  
  RESULTS
  Statistically significant difference was found in mir-499 level before CPB and after reperfusion (T1 vs. T4, p=0.041). We further examined the mir-499 levels by using qRT-PCR in all 13 canines at 4 different time points (T1 vs. T4, p=0.029). Mir-499 expression was negatively correlated with cardiac troponin T (cTnT) and creatine kinase- MB (CK-MB) levels of canines in all time points samples (r=0.469, p<0.001 and r=0.273, p=0.050 respectively). Moreover, higher mir-499 expression level was associated with higher dP/dtmax at 25 minutes and 90 minutes after reperfusion.
  
  
  CONCLUSIONS
  Myocardial ischemic reperfusion injury with cardiopulmonary bypass results in declining level of mir-499 expression in left ventricle myocardium of canines, suggesting mir-499 would be a potential therapeutic target in cardiac protection during open heart surgery.
authors.forename: Han
authors.initials: H
authors.lastname: Qin
authors.forename: Guang-xian
authors.initials: GX
authors.lastname: Chen
authors.forename: Meng-ya
authors.initials: MY
authors.lastname: Liang
authors.forename: Jian
authors.initials: J
authors.lastname: Rong
authors.forename: Jian-ping
authors.initials: JP
authors.lastname: Yao
authors.forename: Hai
authors.initials: H
authors.lastname: Liu
authors.forename: Zhong-kai
authors.initials: ZK
authors.lastname: Wu
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002315:Cardiopulmonary Bypass; D052279:Creatine Kinase, MB Form / Q000097:blood; D004195:Disease Models, Animal; D004285:Dogs; D005260:Female; D020869:Gene Expression Profiling; D006439:Hemodynamics; D008297:Male; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D015428:Myocardial Reperfusion Injury / Q000097:blood / Q000235:genetics* / Q000503:physiopathology; D020411:Oligonucleotide Array Sequence Analysis; D015203:Reproducibility of Results; D013997:Time Factors; D020107:Troponin T / Q000097:blood
chemical_list: D015415:Biomarkers; D035683:MicroRNAs; D020107:Troponin T; D052279:Creatine Kinase, MB Form