title: A novel fluorescence-quenching immunochromatographic sensor for detection of the heavy metal chromium.
abstract: A novel fluorescence quenching immunochromatographic sensor (ICS) was developed for detecting chromium (Cr(3+)) within 15 min utilizing the fluorescence quenching function of gold nanoparticles (Au-NPs). The sensor performed with a positive readout. When the low concentrations of Cr(3+) samples were applied, detection signals of the test line (T line) were quenched, whereas when higher concentration Cr(3+) samples (1.56 ng/mL) were applied, the detection signal of the T line appeared. The detection signal intensity of the T line increased with increasing concentrations of Cr(3+). The low detection limit of developed fluorescence quenching ICS was 1.56 ng/mL. The fluorescence quenching ICS has a linear range of detection of Cr(3+) comprising between 6.25 ng/mL to 800 ng/mL. The recoveries of the fluorescence quenching ICS to detect Cr(3+) in tap water ranged from 94.7% to 101.7%. This result indicated that the developed sensor gave higher sensitivity and reliable reproducibility. It could provide a general detection method for small analyte in water samples.
authors.affiliation: Department of Bioengineering, Guangdong Province Key Laboratory of Molecular Immunology and Antibody Engineering, Jinan University, Guangzhou 510632, PR China.
authors.forename: QiangQiang
authors.initials: Q
authors.lastname: Fu
authors.forename: Yong
authors.initials: Y
authors.lastname: Tang
authors.forename: CongYing
authors.initials: C
authors.lastname: Shi
authors.forename: XiaoLi
authors.initials: X
authors.lastname: Zhang
authors.forename: JunJian
authors.initials: J
authors.lastname: Xiang
authors.forename: Xi
authors.initials: X
authors.lastname: Liu
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation*; D002846:Chromatography, Affinity / Q000295:instrumentation*; D002857:Chromium / Q000032:analysis*; D060766:Drinking Water / Q000032:analysis*; D004867:Equipment Design; D006046:Gold / Q000737:chemistry; D057230:Limit of Detection; D053758:Nanoparticles / Q000737:chemistry; D015203:Reproducibility of Results
chemical_list: D060766:Drinking Water; D002857:Chromium; D006046:Gold
keywords: Chromium; Competitive immunoreaction; Fluorescence quenching; Immunochromatographic sensor; Small analytes