title: Dodoviscin a inhibits melanogenesis in mouse b16-f10 melanoma cells.
abstract: Nowadays, abnormal hyperpigmentation in human skin such as melasma, freckles, and chloasma has become a serious esthetic problem. Cutaneous depigmenting agents could be used to treat these hyperpigmentation-associated dieseases. Dodoviscin A is a natural product isolated from the aerial parts of Dodonaea viscosa. In the present study, we evaluated the effect of dodoviscin A on melanin production in B16-F10 melanoma cells for the first time. We found that dodoviscin A inhibited melanin biosynthesis induced by 3-isobutyl-1-methylxanthine and PD98059 significantly, and there was no obvious effect on the viability of dodoviscin A-treated B16-F10 cells. Meanwhile, dodoviscin A could suppress the activity of mushroom tyrosinase in the cell-free assay system and also decrease 3-isobutyl-1-methylxanthine-induced tyrosinase activity and expression of mature tyrosinase protein in B16-F10 cells. Western blotting analysis showed that dodoviscin A inhibited 3-isobutyl-1-methylxanthine and forskolin-induced phosphorylation of the cAMP response element binding protein in B16-F10 cells. These results indicate that dodoviscin A may be a new promising pigmentation-altering agent for cosmetic and therapeutic applications.
authors.affiliation: Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, China.
authors.forename: Guirui
authors.initials: G
authors.lastname: Yan
authors.forename: Jingjie
authors.initials: J
authors.lastname: Zhu
authors.forename: Laibin
authors.initials: L
authors.lastname: Zhang
authors.forename: Zhijian
authors.initials: Z
authors.lastname: Xu
authors.forename: Gaihong
authors.initials: G
authors.lastname: Wang
authors.forename: Weiliang
authors.initials: W
authors.lastname: Zhu
authors.forename: Aijun
authors.initials: A
authors.lastname: Hou
authors.forename: Heyao
authors.initials: H
authors.lastname: Wang
mesh_terms: D015056:1-Methyl-3-isobutylxanthine / Q000494:pharmacology; D000818:Animals; D045744:Cell Line, Tumor; D002470:Cell Survival; D017362:Cyclic AMP Response Element-Binding Protein / Q000187:drug effects / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005419:Flavonoids / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D005656:Fungal Proteins / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D008543:Melanins / Q000378:metabolism*; D008546:Melanoma, Experimental; D051379:Mice; D008958:Models, Molecular; D014442:Monophenol Monooxygenase / Q000187:drug effects* / Q000378:metabolism; D010766:Phosphorylation; D035261:Plant Components, Aerial / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D054876:Prenylation; D029631:Sapindaceae / Q000737:chemistry*; D012880:Skin Pigmentation / Q000187:drug effects*
chemical_list: D017362:Cyclic AMP Response Element-Binding Protein; D005419:Flavonoids; D005656:Fungal Proteins; D008543:Melanins; D010936:Plant Extracts; D014442:Monophenol Monooxygenase; C093973:2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one; D015056:1-Methyl-3-isobutylxanthine