title: Biodegradable drug-eluting poly[lactic-co-glycol acid] nanofibers for the sustainable delivery of vancomycin to brain tissue: in vitro and in vivo studies.
abstract: Successful treatment of a brain infection requires aspiration of the pus or excision of the abscess, followed by long-term (usually 4-8 weeks) parenteral antibiotic treatment. Local antibiotic delivery using biodegradable drug-impregnated carriers is effective in treating postoperative infections, thereby reducing the toxicity associated with parenteral antibiotic treatment and the expense involved with long-term hospitalization. We have developed vancomycin-loaded, biodegradable poly[lactic-co-glycol acid] nanofibrous membranes for the sustainable delivery of vancomycin to the brain tissue of rats by using the electrospinning technique. A high-performance liquid chromatography assay was employed to characterize the in vitro and in vivo release behaviors of pharmaceuticals from the membranes. The experimental results suggested that the biodegradable nanofibers can release high concentrations of vancomycin for more than 8 weeks in the cerebral cavity of rats. Furthermore, the membranes can cover the wall of the cavity after the removal of abscess more completely and achieve better drug delivery without inducing adverse mass effects in the brain. Histological examination also showed no inflammation reaction of the brain tissues. By adopting the biodegradable, nanofibrous drug-eluting membranes, we will be able to achieve long-term deliveries of various antibiotics in the cerebral cavity to enhance the therapeutic efficacy of cerebral infections.
authors.affiliation: Department of Neurosurgery, Shuang Ho Hospital, Taipei Medical University , Taipei, Taiwan.
authors.forename: Yuan-Yun
authors.initials: YY
authors.lastname: Tseng
authors.forename: Yu-Chun
authors.initials: YC
authors.lastname: Kao
authors.forename: Jun-Yi
authors.initials: JY
authors.lastname: Liao
authors.forename: Wei-An
authors.initials: WA
authors.lastname: Chen
authors.forename: Shih-Jung
authors.initials: SJ
authors.lastname: Liu
mesh_terms: D020341:Absorbable Implants; D000042:Absorption; D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage* / Q000009:adverse effects / Q000627:therapeutic use; D001921:Brain / Q000187:drug effects* / Q000648:ultrastructure; D001922:Brain Abscess / Q000188:drug therapy*; D002851:Chromatography, High Pressure Liquid; D003131:Combined Modality Therapy; D004337:Drug Carriers; D004353:Drug Evaluation, Preclinical; D004343:Drug Implants; D019344:Lactic Acid; D008567:Membranes, Artificial; D057139:Nanofibers; D011100:Polyglycolic Acid; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D016062:Porosity; D011897:Random Allocation; D051381:Rats; D014640:Vancomycin / Q000008:administration & dosage* / Q000009:adverse effects / Q000627:therapeutic use; D014945:Wound Healing
chemical_list: D000900:Anti-Bacterial Agents; D004337:Drug Carriers; D004343:Drug Implants; D008567:Membranes, Artificial; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D014640:Vancomycin