title: Selective identification of macrophages and cancer cells based on thermal transport through surface-imprinted polymer layers.
abstract: In this article, we describe a novel straightforward method for the specific identification of viable cells (macrophages and cancer cell lines MCF-7 and Jurkat) in a buffer solution. The detection of the various cell types is based on changes of the heat transfer resistance at the solid-liquid interface of a thermal sensor device induced by binding of the cells to a surface-imprinted polymer layer covering an aluminum chip. We observed that the binding of cells to the polymer layer results in a measurable increase of heat transfer resistance, meaning that the cells act as a thermally insulating layer. The detection limit was found to be on the order of 10(4) cells/mL, and mutual cross-selectivity effects between the cells and different types of imprints were carefully characterized. Finally, a rinsing method was applied, allowing for the specific detection of cancer cells with their respective imprints while the cross-selectivity toward peripheral blood mononuclear cells was negligible. The concept of the sensor platform is fast and low-cost while allowing also for repetitive measurements.
authors.affiliation: Hasselt University, Institute for Materials Research IMO, Wetenschapspark 1, B-3590 Diepenbeek, Belgium.
authors.forename: Kasper
authors.initials: K
authors.lastname: Eersels
authors.forename: Bart
authors.initials: B
authors.lastname: van Grinsven
authors.forename: Anitha
authors.initials: A
authors.lastname: Ethirajan
authors.forename: Silke
authors.initials: S
authors.lastname: Timmermans
authors.forename: Kathia L
authors.initials: KL
authors.lastname: Jiménez Monroy
authors.forename: Jeroen F J
authors.initials: JF
authors.lastname: Bogie
authors.forename: Sathya
authors.initials: S
authors.lastname: Punniyakoti
authors.forename: Thijs
authors.initials: T
authors.lastname: Vandenryt
authors.forename: Jerome J A
authors.initials: JJ
authors.lastname: Hendriks
authors.forename: Thomas J
authors.initials: TJ
authors.lastname: Cleij
authors.forename: Mat J A P
authors.initials: MJ
authors.lastname: Daemen
authors.forename: Veerle
authors.initials: V
authors.lastname: Somers
authors.forename: Ward
authors.initials: W
authors.lastname: De Ceuninck
authors.forename: Patrick
authors.initials: P
authors.lastname: Wagner
mesh_terms: D000818:Animals; D032701:Biomimetics; D002460:Cell Line; D045744:Cell Line, Tumor; D004867:Equipment Design; D006358:Hot Temperature; D006801:Humans; D019169:Jurkat Cells; D007963:Leukocytes, Mononuclear / Q000166:cytology; D061986:MCF-7 Cells; D008264:Macrophages / Q000378:metabolism* / Q000473:pathology*; D051379:Mice; D054802:Molecular Imprinting; D009369:Neoplasms / Q000378:metabolism* / Q000473:pathology*; D011108:Polymers / Q000737:chemistry; D011140:Polyurethanes / Q000737:chemistry; D051381:Rats; D013499:Surface Properties
chemical_list: D011108:Polymers; D011140:Polyurethanes