title: Poly (ADP-ribose) polymerase 1 protein expression in normal and neoplastic prostatic tissue.
abstract: A genetic background has been implicated in the development of prostate cancer. Protein microarrays have enabled the identification of proteins, some of which associated with apoptosis, that may play a role in the development of such a tumor. Inhibition of apoptosis is a co-factor that contributes to the onset and progression of prostate cancer, though the molecular mechanisms are not entirely understood. Poly (ADP-ribose) polymerase 1 (PARP-1) gene is required for translocation of the apoptosis-inducing factor (AIF) from the mitochondria to the nucleus. Hence, it is involved in programmed cell death. Different PARP-1 gene expression has been observed in various tumors such as glioblastoma, lung, ovarian, endometrial, and skin cancers. We evaluated the expression of PARP-1 protein in prostatic cancer and normal prostate tissues by immunohistochemistry in 40 men with prostate cancer and in 37 normal men. Positive nuclear PARP-1 staining was found in all samples (normal prostate and prostate cancer tissues). No cytoplasmic staining was observed in any sample. PARP-1-positive cells resulted significantly higher in patients with prostate carcinoma compared with controls (P&lt;0.001). PARP-1 over-expression in prostate cancer tissue compared with normal prostate suggests a greater activity of PARP-1 in these tumors. These findings suggest that PARP-1 expression in prostate cancer is an attempt to trigger apoptosis in this type of tumor similarly to what reported in other cancers.
authors.affiliation: Section of Endocrinology, Andrology and Internal Medicine, Department of Medical and Pediatric Sciences, University of Catania, 95123 Catania, Italy. micezia@tiscali.it
authors.forename: M
authors.initials: M
authors.lastname: Salemi
authors.forename: A
authors.initials: A
authors.lastname: Galia
authors.forename: F
authors.initials: F
authors.lastname: Fraggetta
authors.forename: C
authors.initials: C
authors.lastname: La Corte
authors.forename: P
authors.initials: P
authors.lastname: Pepe
authors.forename: S
authors.initials: S
authors.lastname: La Vignera
authors.forename: G
authors.initials: G
authors.lastname: Improta
authors.forename: P
authors.initials: P
authors.lastname: Bosco
authors.forename: A E
authors.initials: AE
authors.lastname: Calogero
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D017209:Apoptosis; D002277:Carcinoma / Q000201:enzymology / Q000473:pathology; D002467:Cell Nucleus / Q000201:enzymology; D003593:Cytoplasm / Q000201:enzymology; D015971:Gene Expression Regulation, Enzymologic; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D000071137:Poly (ADP-Ribose) Polymerase-1; D011065:Poly(ADP-ribose) Polymerases / Q000235:genetics* / Q000378:metabolism*; D011467:Prostate / Q000201:enzymology*; D011471:Prostatic Neoplasms / Q000201:enzymology* / Q000473:pathology
chemical_list: C499320:PARP1 protein, human; D000071137:Poly (ADP-Ribose) Polymerase-1; D011065:Poly(ADP-ribose) Polymerases