title: Evaluation of a new chromogenic agar medium for Alicyclobacillus acidoterrestris.
abstract: Spoilage of fruit juices by a thermoacidophilic spore-forming bacterium, Alicyclobacillus acidoterrestris, is a big problem for fruit juice industries worldwide. We have developed a novel chromogenic selective agar medium (EAATSM) for the isolation and enumeration of A. acidoterrestris. A. acidoterrestris strains appeared as blue colonies on the EAATSM. Other Alicyclobacillus strains appeared as white colonies or were inhibited. A study comparing EAATSM and YSG agar was carried out using artificially contaminated samples of 50 fruit juice products. The correlation coefficient between EAATSM and YSG was 0.991.
authors.affiliation: Nissui Pharmaceutical Co., Ltd. 1075-2 Hokunanmoro, Yuki, Ibaraki 307-0036, Japan. m-uchida@yki.nissui-pharm.jp
authors.forename: Masafumi
authors.initials: M
authors.lastname: Uchida
authors.forename: Hajime
authors.initials: H
authors.lastname: Teramura
authors.forename: Mitsuaki
authors.initials: M
authors.lastname: Kashida
authors.forename: Hidemasa
authors.initials: H
authors.lastname: Kodaka
mesh_terms: D056450:Alicyclobacillus / Q000254:growth & development* / Q000302:isolation & purification / Q000378:metabolism; D001628:Beverages / Q000382:microbiology*; D002863:Chromogenic Compounds / Q000378:metabolism; D015169:Colony Count, Microbial / Q000379:methods*; D003470:Culture Media / Q000737:chemistry* / Q000378:metabolism; D005506:Food Contamination / Q000032:analysis; D005516:Food Microbiology / Q000295:instrumentation / Q000379:methods*
chemical_list: D002863:Chromogenic Compounds; D003470:Culture Media