title: Determination of chiral jasmonates in flowers by GC/MS after monolithic material sorptive extraction.
abstract: A GC/MS method with monolithic material sorptive extraction (MMSE) pretreatment was developed to determine contents of the enantiomers of jasmonic acid and methyl jasmonate in flowers. To optimize MMSE extraction, several MMSE parameters were investigated, including extraction temperature, extraction time, and extraction solvent. Under the optimal conditions, extraction efficiency was good. Using the selected-ion monitoring mode, the limit of detection (LOD, S/N = 3) for methyl jasmonates was 0.257 ng/mL. The limit of quantitation (LOQ, S/N = 10) was 0.856 ng/mL. The linearity range was 1-100 ng/mL. The average recovery of methyl jasmonate at lower concentration was 116.8% (2 ng/mL). The relative standard deviation of methyl jasmonate contents determined within the linear range of detection was less than or equal to 15% of the mean determined level. The proposed method is rapid, sensitive, and competently applied to the determination of jasmonic acid and methyl jasmonate enantiomers in flowers.
authors.affiliation: Key Laboratory of Combinatorial Biosynthesis and Drug Discovery (Wuhan University), Ministry of Education, and Wuhan University School of Pharmaceutical Sciences, Wuhan 430071, China.
authors.forename: Wen
authors.initials: W
authors.lastname: Ma
authors.forename: Shuai
authors.initials: S
authors.lastname: Fu
authors.forename: Yuki
authors.initials: Y
authors.lastname: Hashi
authors.forename: Zilin
authors.initials: Z
authors.lastname: Chen
mesh_terms: D000085:Acetates / Q000032:analysis* / Q000737:chemistry; D002681:China; D003517:Cyclopentanes / Q000032:analysis* / Q000737:chemistry; D035264:Flowers / Q000737:chemistry*; D054883:Oxylipins / Q000032:analysis* / Q000737:chemistry; D010675:Pheromones / Q000032:analysis / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry* / Q000302:isolation & purification; D013237:Stereoisomerism
chemical_list: D000085:Acetates; D003517:Cyclopentanes; D054883:Oxylipins; D010675:Pheromones; D010936:Plant Extracts; C011006:jasmonic acid; C072239:methyl jasmonate