title: Effect of increasing levels of zinc fortificant on the iron absorption of bread co-fortified with iron and zinc consumed with a black tea.
abstract: Iron (Fe) and zinc's (Zn) interaction at the absorptive level can have an effect on the success of co-fortification of wheat flour with both minerals on iron deficiency prevention. The aim of the study was to determine the effect of increasing levels of zinc fortificant on the iron absorption of bread co-fortified with iron and zinc consumed with a black tea. Twelve women aged 33-42 years participated in the study. They received on four different days 200 mL of black tea and 100 g of bread made with wheat flour (70% extraction) fortified with either 30 mg Fe/kg alone, as ferrous sulfate (A), or with the same Fe-fortified flour, but with graded levels of Zn, as zinc sulfate: 30 mg/kg (B), 60 mg/kg (C), or 90 mg/kg (D). Fe radioisotopes ((59)Fe and (55)Fe) of high specific activity were used as tracers, and Fe absorption iron was measured by the incorporation of radioactive Fe into erythrocytes. The geometric mean and range of ±1 SD of Fe absorption were as follows: A = 6.5% (2.2-19.3%), B = 4.6% (1.0-21.0%), C = 2.1% (0.9-4.9%), and D = 2.2% (0.7-6.6%), respectively; ANOVA for repeated measures F = 10.9, p < 0.001 (Scheffè's post hoc test: A vs. C, A vs. D, B vs. C, and B vs. D; p < 0.05). We can conclude that Fe absorption of bread made from low-extraction flour fortified with 30 mg/kg of Fe, as ferrous sulfate, and co-fortified with zinc, as zinc sulfate consumed with black tea is significantly decreased at a zinc fortification level of ≥60 mg/kg flour.
authors.affiliation: Institute of Nutrition and Food Technology, University of Chile, Santiago, Chile. molivare@inta.uchile.cl
authors.forename: Manuel
authors.initials: M
authors.lastname: Olivares
authors.forename: Carla
authors.initials: C
authors.lastname: Castro
authors.forename: Fernando
authors.initials: F
authors.lastname: Pizarro
authors.forename: Daniel López
authors.initials: DL
authors.lastname: de Romaña
mesh_terms: D000328:Adult; D000704:Analysis of Variance; D001628:Beverages; D001939:Bread; D018592:Cross-Over Studies; D005260:Female; D005433:Flour; D005527:Food, Fortified; D006801:Humans; D007408:Intestinal Absorption; D007501:Iron / Q000378:metabolism* / Q000493:pharmacokinetics; D007504:Iron Radioisotopes / Q000378:metabolism / Q000493:pharmacokinetics; D013662:Tea / Q000737:chemistry*; D015032:Zinc / Q000378:metabolism*; D019287:Zinc Sulfate / Q000378:metabolism
chemical_list: D007504:Iron Radioisotopes; D013662:Tea; D019287:Zinc Sulfate; D007501:Iron; D015032:Zinc