title: Brevetoxin in blood, biological fluids, and tissues of sea turtles naturally exposed to Karenia brevis blooms in central west Florida.
abstract: In 2005 and 2006, the central west Florida coast experienced two intense Karenia brevis red tide events lasting from February 2005 through December 2005 and August 2006 through December 2006. Strandings of sea turtles were increased in the study area with 318 turtles (n = 174, 2005; n = 144, 2006) stranding between 1 January 2005 and 31 December 2006 compared to the 12-yr average of 43 +/- 23 turtles. Live turtles (n = 61) admitted for rehabilitation showed clinical signs including unresponsiveness, paresis, and circling. Testing of biological fluids and tissues for the presence of brevetoxin activity by enzyme-linked immunosorbent assay found toxin present in 93% (52 of 56) of live stranded sea turtles, and 98% (42 of 43) of dead stranded sea turtles tested. Serial plasma samples were taken from several live sea turtles during rehabilitation and toxin was cleared from the blood within 5-80 days postadmit depending upon the species tested. Among dead animals the highest brevetoxin levels were found in feces, stomach contents, and liver. The lack of significant pathological findings in the majority of animals necropsied supports toxin-related mortality.
authors.affiliation: Mote Marine Laboratory, 1600 Ken Thompson Parkway, Sarasota, Florida 34236, USA. fauquierd@gmail.com
authors.forename: Deborah A
authors.initials: DA
authors.lastname: Fauquier
authors.forename: Leanne J
authors.initials: LJ
authors.lastname: Flewelling
authors.forename: Jennifer
authors.initials: J
authors.lastname: Maucher
authors.forename: Charles A
authors.initials: CA
authors.lastname: Manire
authors.forename: Victoria
authors.initials: V
authors.lastname: Socha
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Kinsel
authors.forename: Brian A
authors.initials: BA
authors.lastname: Stacy
authors.forename: Michael
authors.initials: M
authors.lastname: Henry
authors.forename: Janet
authors.initials: J
authors.lastname: Gannon
authors.forename: John S
authors.initials: JS
authors.lastname: Ramsdell
authors.forename: Jan H
authors.initials: JH
authors.lastname: Landsberg
mesh_terms: D000818:Animals; D001826:Body Fluids / Q000737:chemistry*; D004141:Dinoflagellida / Q000378:metabolism*; D005068:Eutrophication; D005260:Female; D005431:Florida; D008297:Male; D008387:Marine Toxins / Q000097:blood* / Q000737:chemistry / Q000378:metabolism; D035964:Oxocins / Q000097:blood* / Q000737:chemistry / Q000378:metabolism; D013997:Time Factors; D014426:Turtles / Q000097:blood*; D000095702:Polyether Toxins
chemical_list: D008387:Marine Toxins; D035964:Oxocins; C053342:brevetoxin; D000095702:Polyether Toxins