title: Wheat storage proteins in transgenic rice endosperm.
abstract: Transgenic rice seed expressing wheat HMW glutenin subunit was characterized to study the effects of the wheat prolamin on the protein expression pattern and protein size distribution in the endosperm and the functional and rheological properties of the rice flour and dough. Significant differences were found in the protein expression pattern between the transgenic and wild type samples. Comparing the protein expression profiles of transgenic and nontransgenic plants, combined with proteomic-based studies, indicated increased protein disulfide isomerase (PDI) levels in the transgenic rice lines. The accurate molecular size of HMW-GS in rice endosperm was identified by MALDI-TOF-MS analysis. The expressed wheat HMW (subunit 1Dx5) GS showed a positive effect on the functional properties of rice dough by significantly increasing the size distribution of the polymeric protein fraction and modifying the dough mixing parameters.
authors.affiliation: Department of Plant Physiology and Molecular Plant Biology, Eötvös Loránd University , Budapest, Hungary.
authors.forename: Mária
authors.initials: M
authors.lastname: Oszvald
authors.forename: Gábor
authors.initials: G
authors.lastname: Balázs
authors.forename: Sára
authors.initials: S
authors.lastname: Pólya
authors.forename: Sándor
authors.initials: S
authors.lastname: Tömösközi
authors.forename: Rudi
authors.initials: R
authors.lastname: Appels
authors.forename: Ferenc
authors.initials: F
authors.lastname: Békés
authors.forename: László
authors.initials: L
authors.lastname: Tamás
mesh_terms: D001939:Bread / Q000032:analysis; D056625:Endosperm / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D005433:Flour / Q000032:analysis; D015870:Gene Expression; D005983:Glutens / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D008970:Molecular Weight; D012275:Oryza / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism*; D014908:Triticum / Q000235:genetics*
chemical_list: D005983:Glutens; C037302:glutenin