title: Marker gene screening for human mesenchymal stem cells in early osteogenic response to bone morphogenetic protein 6 with DNA microarray.
abstract: OBJECTIVE
  Microarray data were analyzed using bioinformatic tools to screen marker genes of human mesenchymal stem cells (hMSC) in response to bone morphogenetic protein 6 (BMP6).
  
  
  RESULTS
  A total of 190 differentially expressed genes were identified. The interaction network was divided into three functional modules. These genes were connected with BMP signaling pathways and regulation of cell processes, while NOG and BMPR2 participated in the transforming growth factor-beta signal pathway. Besides, several related small molecules were acquired.
  
  
  CONCLUSIONS
  Marker genes in osteogenic responses to BMP6 treatment for hMSC were screened with microarray data along with elaborate function analysis by bioinformatics. NOG and BMPR2 showed potential to become indicators to monitor the directed differentiation of hMSC into osteoblasts, which can be used for bone disease treatment. Moreover, small molecules such as W-13 were retrieved and provided directions for future drug design.
authors.affiliation: Department of Gynecology, Obstetrics and Gynecology Hospital of Fudan University, Shanghai 200011, China.
authors.forename: Shien
authors.initials: S
authors.lastname: Zou
authors.forename: Shaofen
authors.initials: S
authors.lastname: Zhang
authors.forename: Qiqi
authors.initials: Q
authors.lastname: Long
authors.forename: Yuankui
authors.initials: Y
authors.lastname: Cao
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
mesh_terms: D015415:Biomarkers / Q000378:metabolism; D055418:Bone Morphogenetic Protein 6 / Q000378:metabolism / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects* / Q000502:physiology; D005260:Female; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000187:drug effects* / Q000502:physiology; D005819:Genetic Markers; D006801:Humans; D008297:Male; D059630:Mesenchymal Stem Cells; D020411:Oligonucleotide Array Sequence Analysis; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects* / Q000502:physiology; D013449:Sulfonamides / Q000494:pharmacology
chemical_list: C525816:BMP6 protein, human; D015415:Biomarkers; D055418:Bone Morphogenetic Protein 6; D005819:Genetic Markers; D013449:Sulfonamides; C033772:N-(4-aminobutyl)-5-chloro-2-naphthalenesulfonamide