title: Solid phase click ligation for the synthesis of very long oligonucleotides.
abstract: Oligonucleotides have been ligated efficiently on solid-phase using CuAAC and SPAAC chemistry to produce up to 186-mer triazole linked DNA products. Multiple sequential ligation reactions can be carried out by using a masked azide approach. This work suggests a novel modular approach to the synthesis of large complex oligonucleotide analogues.
authors.affiliation: School of Chemistry, University of Southampton, Highfield, Southampton SO17 1BJ, UK.
authors.forename: Jieqiong
authors.initials: J
authors.lastname: Qiu
authors.forename: Afaf H
authors.initials: AH
authors.lastname: El-Sagheer
authors.forename: Tom
authors.initials: T
authors.lastname: Brown
mesh_terms: D057930:Click Chemistry; D015394:Molecular Structure; D009841:Oligonucleotides / Q000138:chemical synthesis* / Q000737:chemistry
chemical_list: D009841:Oligonucleotides