title: Associations of the PTEN -9C>G polymorphism with insulin sensitivity and central obesity in Chinese.
abstract: BACKGROUND
  Phosphatase and tensin homolog on chromosome 10 gene (PTEN) is known as a tumor-suppressor gene. Previous studies demonstrated that PTEN dysfunction affects the function of insulin. However, investigations of PTEN single nucleotide polymorphisms (SNPs) and IR-related disease associations are limited. The aim of the present study was to investigate whether its polymorphism could be involved in the risk of metabolic syndrome (MetS).
  
  
  METHODS
  The genotype frequency of PTEN -9C>G polymorphism was determined by using a Matrix-Assisted Laser Desorption/Ionization Time of Flight Mass Spectrometry (MALDI-TOF MS) method in 530 subjects with MetS and 202 healthy control subjects of the Han Ethnic Chinese population in a case-control analysis.
  
  
  RESULTS
  The PTEN -9C>G polymorphism was not associated with MetS or its hyperglycemia, hypertension and hypertriglyceridemia components. In the control individuals aged <60 years or ≥60 years, the CG genotype individuals had lower insulin sensitivity than CC individuals (P<0.05). In the <60-year-old MetS group and normal glucose tolerance (NGT) subgroup, the CG individuals had lower insulin sensitivity and higher waist circumference (WC) and waist-height-ratio (WHtR) than CC individuals (P<0.05). Multiple linear regression analysis showed that the PTEN polymorphism (P=0.001) contributed independently to 4.2% (adjusted R(2)) of insulin sensitivity variance (estimated by Matsuda ISI), while age (P=0.004), gender (P=0.000) and the PTEN polymorphism (P=0.032) contributed independently to 5.6% (adjusted R(2)) of WHtR variance.
  
  
  CONCLUSIONS
  The CG genotype of PTEN -9C>G polymorphism was not associated with MetS and some of its components as well. However, it may not only decrease insulin sensitivity in the healthy control and MetS in pre-elderly or NGT subjects, but may also increase the risk of central obesity among these MetS individuals.
authors.affiliation: Division of Endocrinology and Metabolism, West China Hospital of Sichuan University, Chengdu, Sichuan, China.
authors.forename: Qiu
authors.initials: Q
authors.lastname: Yang
authors.forename: Hongyi
authors.initials: H
authors.lastname: Cao
authors.forename: Shugui
authors.initials: S
authors.lastname: Xie
authors.forename: Yuzhen
authors.initials: Y
authors.lastname: Tong
authors.forename: Qibo
authors.initials: Q
authors.lastname: Zhu
authors.forename: Fang
authors.initials: F
authors.lastname: Zhang
authors.forename: Qingguo
authors.initials: Q
authors.lastname: Lü
authors.forename: Yan
authors.initials: Y
authors.lastname: Yang
authors.forename: Daigang
authors.initials: D
authors.lastname: Li
authors.forename: Mei
authors.initials: M
authors.lastname: Chen
authors.forename: Changyong
authors.initials: C
authors.lastname: Yu
authors.forename: Wei
authors.initials: W
authors.lastname: Jin
authors.forename: Yuquan
authors.initials: Y
authors.lastname: Yuan
authors.forename: Nanwei
authors.initials: N
authors.lastname: Tong
mesh_terms: D000368:Aged; D016022:Case-Control Studies; D002681:China; D015331:Cohort Studies; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D007333:Insulin Resistance; D008875:Middle Aged; D056128:Obesity, Abdominal / Q000235:genetics*; D051059:PTEN Phosphohydrolase / Q000235:genetics*; D011110:Polymorphism, Genetic
chemical_list: D051059:PTEN Phosphohydrolase; C494929:PTEN protein, human
keywords: ANOVA; BMI; CI; CVD; Central obesity; DM; HOMA-IR; HOMA-β; HbA1c; IR; Insulin resistance; MALDI-TOF MS; MMAC1; Matrix-Assisted Laser Desorption/Ionization Time of Flight Mass Spectrometry; Matsuda ISI; Matsuda insulin sensitivity index; MetS; Metabolic syndrome; NAFLD; NGT; OGTT; OR; PD; PI3K; PIP2; PIP3; PKA; PKC; PTEN; SNP; TEP1; TGFβ-regulated and epithelial cell-enriched phosphatase 1; WC; WHtR; analysis of variance; body mass index; cardiovascular disease; confidence interval; glycated hemoglobin; homeostasis model assessments of insulin resistance; homeostasis model assessments of β-cell function; insulin resistance; metabolic syndrome; multiple advanced cancers 1; nonalcoholic fatty liver disease; normal glucose tolerance; odds ratio; oral glucose tolerance test; phosphatase and tensin homolog on chromosome 10; phosphatidylinositol (PI) 3-kinase; phosphatidylinositol-3,4,5-trisphosphate; phosphatidylinositol-4,5-bisphosphate; prediabetes; protein kinase A; protein kinase C; single nucleotide polymorphism; type 2 diabetes mellitus; waist circumference; waist circumference/height