title: CYP1B1 C4326G polymorphism and susceptibility to cervical cancer in Chinese Han women.
abstract: Cytochrome P450 1B1 (CYP1B1) is a key P450 enzyme, which could catalyze the formation of 4-hydroxy estrogen metabolites and play a role in estrogen-dependent cancers. We hypothesized that genetic variant in CYP1B1 may modify individual susceptibility to cervical cancer. The aim of this study was to evaluate the association between CYP1B1 C4326G polymorphism and cervical cancer risk in Chinese women. We extracted the peripheral blood samples in 250 patients with cervical cancer and 250 female controls. The matrix-assisted laser desorption ionization time-of-flight mass spectrometry method and direct DNA sequencing were performed to detect the polymorphism. The frequencies of CC, CG, and GG genotypes of CYP1B1 C4326G in cases and controls were 66.0, 26.8, 7.2% and 75.2, 21.6, and 3.2%, respectively, and there was a significant difference between the two groups (P = 0.034). Compared with the wild-type CC genotype, the variant GG genotype was associated with a significantly increased risk of cervical cancer (adjusted OR = 2.30; 95% CI = 1.02, 5.50). Moreover, stratification analysis by age, smoking, drinking, human papillomaviruses (HPV) 16 or 18 carrier status, and family history of cervical cancer, we found that the variant genotypes containing the G allele were associated with a significantly increased risk of cervical cancer among HPV 16 or 18-positive individuals (adjusted OR = 2.85; 95% CI = 1.45, 5.62) and among women younger than 45 years old (adjusted OR = 1.87; 95% CI = 1.03, 3.37). These results suggest that CYP1B1 C4326G polymorphism may increase risk of cervical cancer in Chinese women, especially among young individuals with high-risk HPV infection.
authors.affiliation: Division of Reproductive Medical Center, West China Second University Hospital, Sichuan University, Chengdu, 610041, Sichuan Province, China.
authors.forename: Ya
authors.initials: Y
authors.lastname: Li
authors.forename: Shi-Qiao
authors.initials: SQ
authors.lastname: Tan
authors.forename: Qian-Hong
authors.initials: QH
authors.lastname: Ma
authors.forename: Lei
authors.initials: L
authors.lastname: Li
authors.forename: Zhong-Ying
authors.initials: ZY
authors.lastname: Huang
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.forename: Shang-Wei
authors.initials: SW
authors.lastname: Li
mesh_terms: D000328:Adult; D000367:Age Factors; D000428:Alcohol Drinking; D001189:Aryl Hydrocarbon Hydroxylases / Q000235:genetics*; D044466:Asian People / Q000235:genetics; D016022:Case-Control Studies; D002681:China; D065633:Cytochrome P-450 CYP1B1; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease / Q000208:ethnology / Q000235:genetics*; D005838:Genotype; D054884:Host-Pathogen Interactions; D052162:Human papillomavirus 16 / Q000502:physiology; D052161:Human papillomavirus 18 / Q000502:physiology; D006801:Humans; D016015:Logistic Models; D008875:Middle Aged; D016017:Odds Ratio; D030361:Papillomavirus Infections / Q000150:complications / Q000821:virology; D020641:Polymorphism, Single Nucleotide; D012307:Risk Factors; D012907:Smoking; D002583:Uterine Cervical Neoplasms / Q000150:complications / Q000208:ethnology / Q000235:genetics*
chemical_list: D001189:Aryl Hydrocarbon Hydroxylases; C585496:CYP1B1 protein, human; D065633:Cytochrome P-450 CYP1B1