title: MYC status determination in aggressive B-cell lymphoma: the impact of FISH probe selection.
abstract: OBJECTIVE
  To assess how hybridization probe design may affect MYC status determination in Burkitt lymphoma and diffuse large B-cell lymphoma.
  
  
  RESULTS
  We compared the results obtained with one dual-fusion and two break-apart commercial probes in a retrospective series of 91 aggressive B-cell lymphomas. All three probes were able to detect the IGH-MYC translocation in every case bearing it (13/13). However, seven of 13 (54%) non-IGH-MYC (light-chain immunoglobulin or non-immunoglobulin-MYC) rearrangements were unambiguously detected by just one of the probes tested. On the other hand, when the IGH-MYC dual-fusion probe was used, nine of 15 (60%) cases with a hybridization pattern suggestive of a non-IGH-MYC translocation were attributable to MYC copy gain rather than MYC rearrangement, as demonstrated by both break-apart probes.
  
  
  CONCLUSIONS
  Taking into account the prognostic and therapeutic implications of the MYC translocation, probe design and limitations should be particularly kept in mind when MYC hybridization patterns are interpreted. In our experience, detection of 8q24 abnormalities could be optimized by a two-probe approach involving the application of both IGH-MYC dual-fusion and MYC break-apart selected kits.
authors.affiliation: Department of Pathology, Hospital Universitari Germans Trias i Pujol, Badalona, Barcelona, Spain.
authors.forename: Ana M
authors.initials: AM
authors.lastname: Muñoz-Mármol
authors.forename: Carolina
authors.initials: C
authors.lastname: Sanz
authors.forename: Gustavo
authors.initials: G
authors.lastname: Tapia
authors.forename: Ruth
authors.initials: R
authors.lastname: Marginet
authors.forename: Aurelio
authors.initials: A
authors.lastname: Ariza
authors.forename: José L
authors.initials: JL
authors.lastname: Mate
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002051:Burkitt Lymphoma / Q000235:genetics* / Q000473:pathology*; D015321:Gene Rearrangement; D050438:Genes, Immunoglobulin Heavy Chain; D050439:Genes, Immunoglobulin Light Chain; D016259:Genes, myc; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D016403:Lymphoma, Large B-Cell, Diffuse / Q000235:genetics* / Q000473:pathology*; D008875:Middle Aged; D015341:Nucleic Acid Probes / Q000235:genetics*; D012189:Retrospective Studies; D055815:Young Adult
chemical_list: D015341:Nucleic Acid Probes
keywords: Burkitt lymphoma; MYC; break-apart; diffuse large B-cell lymphomas; dual-fusion; fluorescence in-situ hybridization; translocation