title: Evaluation of dihydroisocoumarins produced by the endophytic fungus Arthrinium state of Apiospora montagnei against Schistosoma mansoni.
abstract: Fractionation of extracts from the fermentation broth of the endophytic fungus Arthrinium state of Apiospora montagnei resulted in the isolation of the major secondary metabolites, R-(-)-mellein (1) and cis-(3R,4R)-4-hydroxymellein (2). The chemical structures of compounds were determined by spectroscopic analyses. The isolated compounds were tested in vitro to determine their activity against Schistosoma mansoni adult worms. Compounds 1 and 2 caused death of 100% of parasites at 200 and 50 μg mL(-1), respectively. Ultrastructural analysis suggested that the tegument can be a target of compound 1.
authors.affiliation: a Departamento de Ciências Farmacêuticas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto , Universidade de São Paulo , Avenida do Café s/n, 14040-903 , Ribeirão Preto SP Brazil.
authors.forename: Henrique P
authors.initials: HP
authors.lastname: Ramos
authors.forename: Marilia R
authors.initials: MR
authors.lastname: Simão
authors.forename: Julia M
authors.initials: JM
authors.lastname: de Souza
authors.forename: Lizandra G
authors.initials: LG
authors.lastname: Magalhães
authors.forename: Vanderlei
authors.initials: V
authors.lastname: Rodrigues
authors.forename: Sérgio R
authors.initials: SR
authors.lastname: Ambrósio
authors.forename: Suraia
authors.initials: S
authors.lastname: Said
mesh_terms: D000818:Animals; D001203:Ascomycota / Q000378:metabolism*; D003470:Culture Media; D004353:Drug Evaluation, Preclinical; D005260:Female; D005285:Fermentation; D049934:Isocoumarins / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D008297:Male; D012550:Schistosoma mansoni / Q000187:drug effects*
chemical_list: D003470:Culture Media; D049934:Isocoumarins