title: Targeted delivery of Epirubicin to cancer cells by PEGylated A10 aptamer.
abstract: Clinical administrations of anthracyclines are limited by cardiotoxicity and myelosuppression. Targeted delivery of anticancer agents is especially important in reducing their side effects. In this work, A10 (Apt), an aptamer for prostate-specific membrane anytigen (PSMA), was applied for targeted delivery of Epirubicin (Epi) to LNCaP cells (PSMA(+)). Flow cytometry analysis showed that PEG-Apt-Epi complex was internalized effectively to LNCaP cells (PSMA(+)), but not to PC3 cells (PSMA(-)). This fact was confirmed by less cytotoxicity of PEG-Apt-Epi complex in PC3 cells in comparison with Epi alone. No significant change in viability between Epi- and complex-treated LNCaP cells was observed. In conclusion, PEG-Apt-Epi complex is an efficient and simple system for specific delivery of drug to PSMA-expressing cell lines.
authors.affiliation: Department of Pharmaceutical Biotechnology, Zabol University of Medical Sciences, Zabol, Iran.
authors.forename: Seyed Mohammad
authors.initials: SM
authors.lastname: Taghdisi
authors.forename: Noor Mohammad
authors.initials: NM
authors.lastname: Danesh
authors.forename: Ahmad
authors.initials: A
authors.lastname: Sarreshtehdar Emrani
authors.forename: Kaveh
authors.initials: K
authors.lastname: Tabrizian
authors.forename: Majid
authors.initials: M
authors.lastname: Zandkarimi
authors.forename: Mohammad
authors.initials: M
authors.lastname: Ramezani
authors.forename: Khalil
authors.initials: K
authors.lastname: Abnous
mesh_terms: D000951:Antigens, Neoplasm / Q000008:administration & dosage / Q000737:chemistry; D000970:Antineoplastic Agents / Q000008:administration & dosage / Q000737:chemistry; D052157:Aptamers, Nucleotide / Q000008:administration & dosage* / Q000737:chemistry*; D044482:Benzeneacetamides / Q000008:administration & dosage* / Q000737:chemistry*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D016503:Drug Delivery Systems / Q000379:methods; D015251:Epirubicin / Q000008:administration & dosage* / Q000737:chemistry*; D006801:Humans; D008297:Male; D010881:Piperidones / Q000008:administration & dosage* / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry*; D011471:Prostatic Neoplasms / Q000188:drug therapy
chemical_list: D000951:Antigens, Neoplasm; D000970:Antineoplastic Agents; D052157:Aptamers, Nucleotide; D044482:Benzeneacetamides; D010881:Piperidones; C052091:antineoplaston A10; D011092:Polyethylene Glycols; D015251:Epirubicin