title: A simple strategy for heritable chromosomal deletions in zebrafish via the combinatorial action of targeting nucleases.
abstract: Precise and effective genome-editing tools are essential for functional genomics and gene therapy. Targeting nucleases have been successfully used to edit genomes. However, whole-locus or element-specific deletions abolishing transcript expression have not previously been reported. Here, we show heritable targeting of locus-specific deletions in the zebrafish nodal-related genes squint (sqt) and cyclops (cyc). Our strategy of heritable chromosomal editing can be used for disease modeling, analyzing gene clusters, regulatory regions, and determining the functions of non-coding RNAs in genomes.
authors.forename: Shimin
authors.initials: S
authors.lastname: Lim
authors.forename: Yin
authors.initials: Y
authors.lastname: Wang
authors.forename: Xueyao
authors.initials: X
authors.lastname: Yu
authors.forename: Yian
authors.initials: Y
authors.lastname: Huang
authors.forename: Mark S
authors.initials: MS
authors.lastname: Featherstone
authors.forename: Karuna
authors.initials: K
authors.lastname: Sampath
mesh_terms: D000483:Alleles; D000818:Animals; D001483:Base Sequence; D002872:Chromosome Deletion; D003433:Crosses, Genetic; D003851:Deoxyribonucleases / Q000378:metabolism*; D004625:Embryo, Nonmammalian / Q000378:metabolism; D005260:Female; D056426:Genetic Loci; D023281:Genomics / Q000379:methods*; D018095:Germ-Line Mutation / Q000235:genetics; D049452:Green Fluorescent Proteins / Q000378:metabolism; D040582:Inheritance Patterns / Q000235:genetics*; D008297:Male; D008969:Molecular Sequence Data; D012313:RNA / Q000235:genetics; D015027:Zebrafish / Q000196:embryology / Q000235:genetics*; D016335:Zinc Fingers / Q000235:genetics
chemical_list: C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; D012313:RNA; D003851:Deoxyribonucleases