title: An enzyme-free and label-free assay for copper(II) ion detection based on self-assembled DNA concatamers and Sybr Green I.
abstract: An enzyme-free and label-free fluorescence turn on biosensor for amplified copper(II) ion (Cu(2+)) detection has been constructed based on self-assembled DNA concatamers and Sybr Green I. This assay is simple, inexpensive and sensitive, enabling quantitative detection of as low as 12.8 pM Cu(2+).
authors.affiliation: Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China.
authors.forename: Chenchen
authors.initials: C
authors.lastname: Ge
authors.forename: Junhua
authors.initials: J
authors.lastname: Chen
authors.forename: Wei
authors.initials: W
authors.lastname: Wu
authors.forename: Zhiyuan
authors.initials: Z
authors.lastname: Fang
authors.forename: Lingbo
authors.initials: L
authors.lastname: Chen
authors.forename: Qi
authors.initials: Q
authors.lastname: Liu
authors.forename: Lin
authors.initials: L
authors.lastname: Wang
authors.forename: Xuerong
authors.initials: X
authors.lastname: Xing
authors.forename: Lingwen
authors.initials: L
authors.lastname: Zeng
mesh_terms: D001483:Base Sequence; D052160:Benzothiazoles; D015374:Biosensing Techniques / Q000379:methods*; D003300:Copper / Q000032:analysis* / Q000737:chemistry; D004247:DNA / Q000737:chemistry* / Q000235:genetics; D003959:Diamines; D005456:Fluorescent Dyes / Q000737:chemistry*; D009930:Organic Chemicals / Q000737:chemistry*; D011804:Quinolines; D013050:Spectrometry, Fluorescence
chemical_list: D052160:Benzothiazoles; D003959:Diamines; D005456:Fluorescent Dyes; D009930:Organic Chemicals; D011804:Quinolines; C098022:SYBR Green I; D003300:Copper; D004247:DNA