title: Validation of circulating miRNA biomarkers for predicting lymph node metastasis in gastric cancer.
abstract: We validated candidate biomarkers using circulating miRNAs by analyzing serum miRNA concentrations from patients with gastric cancer (GC) to predict lymph node (LN) metastasis. In a pilot study, serum levels of miR-21, miR-27a, miR-106b, miR-146a, miR-148a, miR-223, and miR-433 were compared in 10 healthy donors, 16 LN-positive patients with GC, and 15 LN-negative patients with GC. Then, we compared the level of three miRNAs (miR-21, miR-146a, and miR-148a) with the total of 79 GC patients with or without LN metastasis. In the pilot study, miR-21, miR-27a, miR-106b, miR-146a, miR-148a, and miR-223 concentrations from LN-positive patients with GC were significantly different from those of LN-negative patients with GC (P < 0.001, P = 0.003, P = 0.033, P < 0.001, P <0.001, and P = 0.017, respectively). In the validation study, levels of miR-21, miR-146a, and miR-148a increased as pN stage increased (P < 0.001, P = 0.001, and P < 0.001, respectively). Levels of the miRNAs were significantly different between pN0 and pN0 in the pT1 group (P = 0.013, P = 0.004, and P = 0.035, respectively) and among clinical stages (P = 0.001, P = 0.002, and P < 0.001, respectively). No differences in miRNA levels were observed by pT stage, Lauren's classification, sex, or age. Serum concentrations of miR-21, miR-146a, and miR-148a were closely associated with GC pN stage. These serum miRNA levels could be biomarker candidates to predict the presence of LN metastasis.
authors.affiliation: Department of Clinical Laboratory Medicine, Pusan National University School of Medicine and Medical Research Institute, Busan, Republic of Korea.
authors.forename: Shine Young
authors.initials: SY
authors.lastname: Kim
authors.forename: Tae Yong
authors.initials: TY
authors.lastname: Jeon
authors.forename: Chang In
authors.initials: CI
authors.lastname: Choi
authors.forename: Dae Hwan
authors.initials: DH
authors.lastname: Kim
authors.forename: Dong Heon
authors.initials: DH
authors.lastname: Kim
authors.forename: Gwang Ha
authors.initials: GH
authors.lastname: Kim
authors.forename: Dong Yup
authors.initials: DY
authors.lastname: Ryu
authors.forename: Bong Eun
authors.initials: BE
authors.lastname: Lee
authors.forename: Hyung Hoi
authors.initials: HH
authors.lastname: Kim
mesh_terms: D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000097:blood / Q000235:genetics*; D005260:Female; D006801:Humans; D008207:Lymphatic Metastasis; D008297:Male; D035683:MicroRNAs / Q000097:blood / Q000235:genetics*; D008875:Middle Aged; D010865:Pilot Projects; D011379:Prognosis; D012372:ROC Curve; D015203:Reproducibility of Results; D013274:Stomach Neoplasms / Q000175:diagnosis / Q000235:genetics* / Q000473:pathology*
chemical_list: D014408:Biomarkers, Tumor; C542542:MIRN146 microRNA, human; C550570:MIRN148 microRNA, human; C524940:MIRN21 microRNA, human; D035683:MicroRNAs