title: Correlations of noninvasive BOLD and TOLD MRI with pO2 and relevance to tumor radiation response.
abstract: OBJECTIVE
  To examine the potential use of blood oxygenation level dependent (BOLD) and tissue oxygenation level dependent (TOLD) contrast MRI to assess tumor oxygenation and predict radiation response.
  
  
  METHODS
  BOLD and TOLD MRI were performed on Dunning R3327-AT1 rat prostate tumors during hyperoxic gas breathing challenge at 4.7 T. Animals were divided into two groups. In Group 1 (n = 9), subsequent (19) F MRI based on spin lattice relaxation of hexafluorobenzene reporter molecule provided quantitative oximetry for comparison. For Group 2 rats (n = 13) growth delay following a single dose of 30 Gy was compared with preirradiation BOLD and TOLD assessments.
  
  
  RESULTS
  Oxygen (100%O2 ) and carbogen (95%O2 /5%CO2 ) challenge elicited similar BOLD, TOLD and pO2 responses. Strong correlations were observed between BOLD or R2* response and quantitative (19) F pO2 measurements. TOLD response showed a general trend with weaker correlation. Irradiation caused a significant tumor growth delay and tumors with larger changes in TOLD and R1 values upon oxygen breathing exhibited significantly increased tumor growth delay.
  
  
  CONCLUSIONS
  These results provide further insight into the relationships between oxygen sensitive (BOLD/TOLD) MRI and tumor pO2 . Moreover, a larger increase in R1 response to hyperoxic gas challenge coincided with greater tumor growth delay following irradiation.
authors.affiliation: Department of Radiology, The University of Texas Southwestern Medical Center, Dallas, Texas, USA.
authors.forename: Rami R
authors.initials: RR
authors.lastname: Hallac
authors.forename: Heling
authors.initials: H
authors.lastname: Zhou
authors.forename: Rajesh
authors.initials: R
authors.lastname: Pidikiti
authors.forename: Kwang
authors.initials: K
authors.lastname: Song
authors.forename: Strahinja
authors.initials: S
authors.lastname: Stojadinovic
authors.forename: Dawen
authors.initials: D
authors.lastname: Zhao
authors.forename: Timothy
authors.initials: T
authors.lastname: Solberg
authors.forename: Peter
authors.initials: P
authors.lastname: Peschke
authors.forename: Ralph P
authors.initials: RP
authors.lastname: Mason
mesh_terms: D000818:Animals; D014408:Biomarkers, Tumor / Q000378:metabolism*; D008279:Magnetic Resonance Imaging / Q000379:methods*; D008297:Male; D057054:Molecular Imaging / Q000379:methods; D010092:Oximetry / Q000379:methods*; D010100:Oxygen / Q000378:metabolism*; D011471:Prostatic Neoplasms / Q000378:metabolism* / Q000473:pathology / Q000532:radiotherapy*; D051381:Rats; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013223:Statistics as Topic; D016896:Treatment Outcome
chemical_list: D014408:Biomarkers, Tumor; D010100:Oxygen
keywords: BOLD; MRI; carbogen; hypoxia; oximetry; oxygen; radiation response