title: Different peripheral tissue injury induces differential phenotypic changes of spinal activated microglia.
abstract: The purpose of this study is to investigate the possible different cellular marker expression associated with spinal cord microglial activation in different pain models. Immunohistochemistry and western blotting analysis of CD45, CD68, and MHC class I antigen as well as CD11b and Iba-1 in the spinal cord were quantitatively compared among widely used three pain animal models, complete Freund's adjuvant (CFA) injection, formalin injection, and chronic constriction injury (CCI) models. The results showed that significant upregulated expressions of CD45 and MHC class I antigen in spinal microglia as well as morphological changes with increased staining with CD11b and Iba-1 were seen in CCI and formalin models and not found in CFA-induced inflammatory pain model. CD68 expression was only detected in CCI model. Our findings suggested that different peripheral tissue injuries produced differential phenotypic changes associated with spinal microglial activation; peripheral nerve injury might induce spinal microglia to acquire these immunomolecular phenotypic changes.
authors.affiliation: Center for TMD & Orofacial Pain, Peking University School & Hospital of Stomatology, Beijing 100081, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Li
authors.forename: Yong-Hui
authors.initials: YH
authors.lastname: Tan
authors.forename: Alan R
authors.initials: AR
authors.lastname: Light
authors.forename: Kai-Yuan
authors.initials: KY
authors.lastname: Fu
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000235:genetics / Q000276:immunology; D015214:Antigens, Differentiation, Myelomonocytic / Q000235:genetics / Q000276:immunology; D039481:CD11b Antigen / Q000235:genetics / Q000276:immunology; D002135:Calcium-Binding Proteins / Q000235:genetics / Q000276:immunology; D004195:Disease Models, Animal; D005557:Formaldehyde; D005620:Freund's Adjuvant; D005786:Gene Expression Regulation; D015395:Histocompatibility Antigens Class I / Q000235:genetics / Q000276:immunology; D017493:Leukocyte Common Antigens / Q000235:genetics / Q000276:immunology; D008297:Male; D008840:Microfilament Proteins / Q000235:genetics / Q000276:immunology; D017628:Microglia / Q000276:immunology / Q000473:pathology*; D009437:Neuralgia / Q000139:chemically induced / Q000276:immunology / Q000473:pathology*; D010146:Pain / Q000139:chemically induced / Q000276:immunology / Q000473:pathology*; D059348:Peripheral Nerve Injuries / Q000276:immunology / Q000473:pathology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013116:Spinal Cord / Q000276:immunology / Q000473:pathology*; D000097382:CD68 Molecule
chemical_list: D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D039481:CD11b Antigen; D002135:Calcium-Binding Proteins; D005557:Formaldehyde; D005620:Freund's Adjuvant; D015395:Histocompatibility Antigens Class I; D017493:Leukocyte Common Antigens; D008840:Microfilament Proteins; C100745:Aif1 protein, rat; C539355:CD68 protein, rat; D000097382:CD68 Molecule; C516771:Ptprc protein, rat