title: Characteristics of an orange-pigmented bacterium isolated from a hot spring in Kagoshima, Japan.
abstract: In May 2012, strain HNN-6 (=JCM 18566) , a Gram-negative, non-spore-forming, motile and strictly aerobic rod, which produces a pale orange pigment, was isolated from a hot spring water sample obtained in Kagoshima, Japan, by a plating method using R2A medium at 30°C for 7 d. The 16S rRNA gene sequences (1,437bp) of this strain (accession number: AB731137) had a close similarity (99.1%) to Hydrotalea sandarakina AF-51T (JF739858) . Growth occurred at 25-45°C and pH 5.0-8.0, with optimal growth at 40°C and pH 6.0-7.0. Growth did not occur in the presence of ≧2% NaCl. The API 20NE identification system gave positive results for nitrate, aesculin, gelatin, 4-nitrophenyl-β-D-galactopyranoside, D-glucose, D-mannose, maltose and oxidase (API code number 1472204) . The dominant cellular fatty acids of strain HNN-6 were iso-C15:0 (32.6%) , iso-C17:0 3-OH (24.2%) and iso-C16:0 (8.4%) . The guanine-plus-cytosine (G+C) content of DNA was 36.2 mol%. This article is the first report to describe the characteristics of an orange-pigmented bacterium isolated from a hot spring water sample in Japan.
authors.affiliation: School of Life and Environmental Science, Azabu University, Sagamihara, Kanagawa 252-5201, Japan.
authors.forename: Katsunori
authors.initials: K
authors.lastname: Furuhata
authors.forename: Naoto
authors.initials: N
authors.lastname: Ishizaki
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Handa
authors.forename: Miyuki
authors.initials: M
authors.lastname: Nishijima
authors.forename: Masafumi
authors.initials: M
authors.lastname: Fukuyama
mesh_terms: D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D001482:Base Composition; D005227:Fatty Acids / Q000378:metabolism; D045482:Hot Springs / Q000382:microbiology*; D007564:Japan; D008969:Molecular Sequence Data; D010802:Phylogeny; D010860:Pigments, Biological / Q000378:metabolism*
chemical_list: D005227:Fatty Acids; D010860:Pigments, Biological