title: The significance of p53 immunoexpression with different clones (DO-7 and PAb-240) in oral squamous cell carcinoma.
abstract: OBJECTIVE
  The TP53 gene is a tumor suppressor gene. Its product is a nuclear protein that regulates cell cycle arrest, apoptosis and DNA repair. Anti-p53 clones DO-7 and PAb-240 recognize the amino acid sequences 21-25 and 213-217, respectively. This study aimed to evaluate the expression of these clones and their relationship with clinicopathological features and survival analysis in oral squamous cell carcinomas (OSCC).
  
  
  METHODS
  Information on 53 primary OSCC was collected at the Brazilian National Cancer Institute. An immunohistochemical method was applied to evaluate p53 expression (DO-7 and PAb-240). Their expression was analyzed quantitatively and correlated with clinicopathological features. Kaplan-Meier survival curves and log rank test were used.
  
  
  RESULTS
  Immunopositivity for DO-7 was present in 64% of the cases, while 58% were positive for PAb-240. There was no correlation between immunoexpression of both antibodies and clinicopathological features or survival analysis. DO-7 expression was significantly higher (p = 0.001) than that of PAb-240.
  
  
  CONCLUSIONS
  There were quantitative differences between the expression of the antibodies studied, which may reflect a different specificity of each one. To confirm immunohistochemical results and estimate the true prognostic role of TP53 in OSCC, it is important to perform mutation analysis.
authors.affiliation: Pathology Graduate Program, Fluminense Federal University, Niterói, Brazil.
authors.forename: José de Assis
authors.initials: Jde A
authors.lastname: Silva Júnior
authors.forename: Danielle Resende
authors.initials: DR
authors.lastname: Camisasca
authors.forename: Vagner
authors.initials: V
authors.lastname: Bernardo
authors.forename: Georgina Severo
authors.initials: GS
authors.lastname: Ribeiro
authors.forename: Fernando Luiz
authors.initials: FL
authors.lastname: Dias
authors.forename: Paulo Antônio Silvestre
authors.initials: PA
authors.lastname: de Faria
authors.forename: Lidia Maria da Fonte
authors.initials: LM
authors.lastname: de Amorim
authors.forename: Simone Queiroz Chaves
authors.initials: SQ
authors.lastname: Lourenço
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000911:Antibodies, Monoclonal / Q000235:genetics; D014408:Biomarkers, Tumor / Q000378:metabolism; D002294:Carcinoma, Squamous Cell / Q000378:metabolism / Q000401:mortality / Q000473:pathology; D016208:Databases, Factual; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D053208:Kaplan-Meier Estimate; D008297:Male; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000378:metabolism / Q000401:mortality / Q000473:pathology; D011379:Prognosis; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism*
chemical_list: D000911:Antibodies, Monoclonal; D014408:Biomarkers, Tumor; C089775:DO-7 monoclonal antibody; C495901:TP53 protein, human; D016159:Tumor Suppressor Protein p53