title: A re-sequencing based assessment of genomic heterogeneity and fast neutron-induced deletions in a common bean cultivar.
abstract: A small fast neutron (FN) mutant population has been established from Phaseolus vulgaris cv. Red Hawk. We leveraged the available P. vulgaris genome sequence and high throughput next generation DNA sequencing to examine the genomic structure of five P. vulgaris cv. Red Hawk FN mutants with striking visual phenotypes. Analysis of these genomes identified three classes of structural variation (SV); between cultivar variation, natural variation within the FN mutant population, and FN induced mutagenesis. Our analyses focused on the latter two classes. We identified 23 large deletions (>40 bp) common to multiple individuals, illustrating residual heterogeneity and regions of SV within the common bean cv. Red Hawk. An additional 18 large deletions were identified in individual mutant plants. These deletions, ranging in size from 40 bp to 43,000 bp, are potentially the result of FN mutagenesis. Six of the 18 deletions lie near or within gene coding regions, identifying potential candidate genes causing the mutant phenotype.
authors.affiliation: Plant Science Research Unit, USDA-Agricultural Research Service St. Paul, MN, USA ; Department of Agronomy and Plant Genetics, University of Minnesota St. Paul, MN, USA.
authors.forename: Jamie A
authors.initials: JA
authors.lastname: O'Rourke
authors.forename: Luis P
authors.initials: LP
authors.lastname: Iniguez
authors.forename: Bruna
authors.initials: B
authors.lastname: Bucciarelli
authors.forename: Jeffrey
authors.initials: J
authors.lastname: Roessler
authors.forename: Jeremy
authors.initials: J
authors.lastname: Schmutz
authors.forename: Phillip E
authors.initials: PE
authors.lastname: McClean
authors.forename: Scott A
authors.initials: SA
authors.lastname: Jackson
authors.forename: Georgina
authors.initials: G
authors.lastname: Hernandez
authors.forename: Michelle A
authors.initials: MA
authors.lastname: Graham
authors.forename: Robert M
authors.initials: RM
authors.lastname: Stupar
authors.forename: Carroll P
authors.initials: CP
authors.lastname: Vance
keywords: DNA-Seq; Phaseolus vulgaris; common bean; fast neutron mutation; natural variation; structural variation