title: Synergistic antibacterial and antibiofilm effect between (+)-medioresinol and antibiotics in vitro.
abstract: In this study, antibacterial effects of (+)-Medioresinol isolated from stem bark of Sambucus williamsii and its synergistic activities in combination with antibiotics such as ampicillin, cefotaxime, and chloramphenicol were tested by antibacterial susceptibility testing and checkerboard assay. (+)-Medioresinol possessed antibacterial effects against antibiotics-susceptible- or antibiotics-resistant strains. Most of combinations between (+)-Medioresinol and each antibiotic showed synergistic interaction (fractional inhibitory concentration index ≤ 0.5) against bacterial strains including antibiotics-resistant Pseudomonas aeruginosa. Furthermore, the antibiofilm effect of (+)-Medioresinol alone or in combination with each antibiotic was investigated. The results indicated that not only (+)-Medioresinol but also its combination with each antibiotic had antibiofilm activities. It concludes that (+)-Medioresinol has potential as a therapeutic agent and adjuvant for treatment of bacterial infection.
authors.affiliation: School of Life Sciences and Biotechnology, College of Natural Sciences, Kyungpook National University, Daehak-ro 80, Buk-gu, Daegu, 702-701, Republic of Korea.
authors.forename: Ji Hong
authors.initials: JH
authors.lastname: Hwang
authors.forename: Hyemin
authors.initials: H
authors.lastname: Choi
authors.forename: In-sok
authors.initials: IS
authors.lastname: Hwang
authors.forename: A Ryun
authors.initials: AR
authors.lastname: Kim
authors.forename: Eun-Rhan
authors.initials: ER
authors.lastname: Woo
authors.forename: Dong Gun
authors.initials: DG
authors.lastname: Lee
mesh_terms: D000900:Anti-Bacterial Agents / Q000008:administration & dosage*; D018441:Biofilms / Q000187:drug effects / Q000254:growth & development*; D002470:Cell Survival / Q000187:drug effects; D004357:Drug Synergism; D017705:Lignans / Q000008:administration & dosage* / Q000302:isolation & purification*; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000502:physiology*; D027923:Sambucus / Q000737:chemistry*
chemical_list: D000900:Anti-Bacterial Agents; D017705:Lignans; C576388:medioresinol