title: GC/MS analysis of high-performance liquid chromatography fractions from Sophora flavescens and Torilis japonica extracts and their in vitro anti-neosporal effects on Neospora caninum.
abstract: We analyzed alcoholic extracts of herbs possessing anti-neosporal activity against Neospora (N.) caninum. To identify the chemical components of Sophora (S.) flavescens and Torilis (T.) japonica associated with anti-neosporal activity, specific fractions were isolated by high-performance liquid chromatography (HPLC). In vitro activity of the fractions against N. caninum was then assessed. Gas chromatography/ mass spectrometry (GC/MS) was used to identify and quantify specific anti-neosporal molecules in the herbal extracts. Almost all HPLC fractions of S. flavescens and T. japonica had higher levels of anti-neosporal activity compared to the not treated control. Active constituents of the extracts were sophoridane, furosardonin A, and tetraisopropylidene-cyclobutane in S. flavescens; 5,17-β-dihydroxy-de-A-estra-5,7,9,14-tetraene, furanodiene, and 9,12-octadecadienoic acid (Z,Z)-(CAS,1) in T. japonica.
authors.affiliation: Pfizer Animal Health, Seoul 135-784, Korea.
authors.forename: Hun-Su
authors.initials: HS
authors.lastname: Seo
authors.forename: Kyoung Hee
authors.initials: KH
authors.lastname: Kim
authors.forename: Dae-Yong
authors.initials: DY
authors.lastname: Kim
authors.forename: Bong-Kyun
authors.initials: BK
authors.lastname: Park
authors.forename: Nam-Shik
authors.initials: NS
authors.lastname: Shin
authors.forename: Jae-Hoon
authors.initials: JH
authors.lastname: Kim
authors.forename: Heejeong
authors.initials: H
authors.lastname: Youn
mesh_terms: D019661:Apiaceae / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D003049:Coccidiostats / Q000737:chemistry*; D005638:Fruit / Q000737:chemistry; D008401:Gas Chromatography-Mass Spectrometry; D018688:Neospora / Q000187:drug effects* / Q000254:growth & development; D010936:Plant Extracts / Q000737:chemistry*; D018517:Plant Roots / Q000737:chemistry; D029909:Sophora / Q000737:chemistry*
chemical_list: D003049:Coccidiostats; D010936:Plant Extracts
keywords: GC/MS; HPLC; Neospora caninum; Sophora flavescens; Torilis japonica