title: Phasic synaptic incorporation of GluR2-lacking AMPA receptors at gonadotropin-releasing hormone neurons is involved in the generation of the luteinizing hormone surge in female rats.
abstract: Reproductive success depends on a robust and appropriately timed preovulatory luteinizing hormone (LH) surge, which is induced by the activation of gonadotropin-releasing hormone (GnRH) neurons in response to positive feedback from increasing estrogen levels. Here we document an increase in postsynaptic GluR2-lacking Ca2+ -permeable AMPA-type glutamate receptors (CP-AMPARs) at synapses on GnRH neurons on the day of proestrus in rats, coincident with the increase in estrogen levels. Functional blockade of CP-AMPARs depressed the synaptic responses only on the day of proestrus and concomitantly attenuated the LH surge. Thus, the phasic synaptic incorporation of postsynaptic CP-AMPARs on GnRH neurons is involved in the generation of the LH surge.
authors.affiliation: Department of Physiology, Yokohama City University Graduate School of Medicine, 3-9 Fukuura, Kanazawa-ku, Yokohama 236-0004, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Tada
authors.forename: Y
authors.initials: Y
authors.lastname: Kuroki
authors.forename: T
authors.initials: T
authors.lastname: Funabashi
authors.forename: Y
authors.initials: Y
authors.lastname: Kamiya
authors.forename: T
authors.initials: T
authors.lastname: Goto
authors.forename: K
authors.initials: K
authors.lastname: Suyama
authors.forename: A
authors.initials: A
authors.lastname: Sano
authors.forename: D
authors.initials: D
authors.lastname: Mitsushima
authors.forename: A M
authors.initials: AM
authors.lastname: Etgen
authors.forename: T
authors.initials: T
authors.lastname: Takahashi
mesh_terms: D000818:Animals; D004967:Estrogens / Q000502:physiology; D005260:Female; D007987:Gonadotropin-Releasing Hormone / Q000378:metabolism*; D066298:In Vitro Techniques; D007986:Luteinizing Hormone / Q000097:blood*; D009474:Neurons / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D018091:Receptors, AMPA / Q000502:physiology*; D012098:Reproduction; D013569:Synapses / Q000502:physiology*
chemical_list: D004967:Estrogens; D018091:Receptors, AMPA; D007987:Gonadotropin-Releasing Hormone; D007986:Luteinizing Hormone; C104722:glutamate receptor ionotropic, AMPA 2
keywords: 1-naphthyl acetyl spermine; 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid; 6-cyano-7-nitroquinoxaline-2,3-dione; ANOVA; CNQX; CP-AMPA receptor; CP-AMPARs; EGTA; GFP; GnRH; HEPES; LH; NASPM; OVX; PPR; Trafficking; analysis of variance; calcium permeable AMPA-type glutamate receptors; estrus cycle; ethylene glycol tetraacetic acid; gonadotropin-releasing hormone; green fluorescent protein; luteinizing hormone; mEPSCs; miniature excitatory postsynaptic currents; ovariectomized; paired-pulse ratio; synapse