title: Multilamellar structures and filament bundles are found on the cell surface during bunyavirus egress.
abstract: Inside cells, viruses build specialized compartments for replication and morphogenesis. We observed that virus release associates with specific structures found on the surface of mammalian cells. Cultured adherent cells were infected with a bunyavirus and processed for oriented sectioning and transmission electron microscopy. Imaging of cell basal regions showed sophisticated multilamellar structures (MLS) and extracellular filament bundles with attached viruses. Correlative light and electron microscopy confirmed that both MLS and filaments proliferated during the maximum egress of new viruses. MLS dimensions and structure were reminiscent of those reported for the nanostructures on gecko fingertips, which are responsible for the extraordinary attachment capacity of these lizards. As infected cells with MLS were more resistant to detachment than control cells, we propose an adhesive function for these structures, which would compensate for the loss of adherence during release of new virus progeny.
authors.affiliation: Cell Structure Laboratory, Centro Nacional de Biotecnología, CNB-CSIC, Campus de Cantoblanco, Madrid, Spain.
authors.forename: Laura
authors.initials: L
authors.lastname: Sanz-Sánchez
authors.forename: Cristina
authors.initials: C
authors.lastname: Risco
mesh_terms: D008841:Actin Cytoskeleton / Q000378:metabolism* / Q000648:ultrastructure; D000818:Animals; D002448:Cell Adhesion; D002460:Cell Line; D002462:Cell Membrane / Q000648:ultrastructure / Q000821:virology; D006224:Cricetinae; D015638:Cytochalasin D / Q000494:pharmacology; D005109:Extracellular Matrix / Q000648:ultrastructure / Q000821:virology; D006801:Humans; D046529:Microscopy, Electron, Transmission; D018715:Microscopy, Video; D019384:Nucleic Acid Synthesis Inhibitors / Q000494:pharmacology; D016851:Orthobunyavirus / Q000502:physiology*; D057074:Virus Release
chemical_list: D019384:Nucleic Acid Synthesis Inhibitors; D015638:Cytochalasin D