title: Factors that drive peptide assembly and fibril formation: experimental and theoretical analysis of Sup35 NNQQNY mutants.
abstract: Residue mutations have substantial effects on aggregation kinetics and propensities of amyloid peptides and their aggregate morphologies. Such effects are attributed to conformational transitions accessed by various types of oligomers such as steric zipper or single β-sheet. We have studied the aggregation propensities of six NNQQNY mutants: NVVVVY, NNVVNV, NNVVNY, VIQVVY, NVVQIY, and NVQVVY in water using a combination of ion-mobility mass spectrometry, transmission electron microscopy, atomic force microscopy, and all-atom molecular dynamics simulations. Our data show a strong correlation between the tendency to form early β-sheet oligomers and the subsequent aggregation propensity. Our molecular dynamics simulations indicate that the stability of a steric zipper structure can enhance the propensity for fibril formation. Such stability can be attained by either hydrophobic interactions in the mutant peptide or polar side-chain interdigitations in the wild-type peptide. The overall results display only modest agreement with the aggregation propensity prediction methods such as PASTA, Zyggregator, and RosettaProfile, suggesting the need for better parametrization and model peptides for these algorithms.
authors.affiliation: Department of Chemistry and Biochemistry, University of California, Santa Barbara, California 93106, United States.
authors.forename: Thanh D
authors.initials: TD
authors.lastname: Do
authors.forename: Nicholas J
authors.initials: NJ
authors.lastname: Economou
authors.forename: Nichole E
authors.initials: NE
authors.lastname: LaPointe
authors.forename: William M
authors.initials: WM
authors.lastname: Kincannon
authors.forename: Christian
authors.initials: C
authors.lastname: Bleiholder
authors.forename: Stuart C
authors.initials: SC
authors.lastname: Feinstein
authors.forename: David B
authors.initials: DB
authors.lastname: Teplow
authors.forename: Steven K
authors.initials: SK
authors.lastname: Buratto
authors.forename: Michael T
authors.initials: MT
authors.lastname: Bowers
mesh_terms: D018625:Microscopy, Atomic Force; D008958:Models, Molecular; D056004:Molecular Dynamics Simulation; D009154:Mutation / Q000235:genetics; D010455:Peptides / Q000737:chemistry* / Q000235:genetics; D017433:Protein Structure, Secondary; D014867:Water / Q000737:chemistry
chemical_list: D010455:Peptides; D014867:Water