title: Cytochrome c oxidase activity and nitric oxide synthase in the rat brain following aluminium intracerebral application.
abstract: In the present study we investigated the cytochrome C oxidase (CO) activity and nitric oxide synthase (NOS) isoenzyme expression after intrahippocampal AlCl3 application in selective vulnerable brain structures. A single dose of AlCl3 was applied in the CA1 sector of rats hippocampus. For biochemical analysis, the animals were killed 10 min and three days after the treatment and forebrain cortex, basal forebrain and hippocampus were removed. Activity of CO was decreased bilaterally in the AlCl3-treated groups in all examined brain structures. We also applied immunohistochemical techniques to identify changes induced by AlCl3 injection after survival periods of 10 min and three days. Both the nNOS and eNOS stains were detected in the hippocampus of controls and AlCl3-treated animals, but iNOS labelling was present in the hippocampus only three days after AlCl3 application. An increased iNOS expression three days post AlCl3 administration could be involved in the mechanism of CO activity depletion.
authors.affiliation: Military Medical Academy, Institute for Medical Research, Belgrade, Serbia. ivanav13@yahoo.ca
authors.forename: Ivana D
authors.initials: ID
authors.lastname: Stevanović
authors.forename: Marina D
authors.initials: MD
authors.lastname: Jovanović
authors.forename: Miodrag
authors.initials: M
authors.lastname: Colić
authors.forename: Milica
authors.initials: M
authors.lastname: Ninković
authors.forename: Ankica
authors.initials: A
authors.lastname: Jelenković
authors.forename: Rosa
authors.initials: R
authors.lastname: Mihajlović
mesh_terms: D000077410:Aluminum Chloride; D017607:Aluminum Compounds / Q000008:administration & dosage*; D000818:Animals; D001921:Brain / Q000187:drug effects / Q000201:enzymology*; D002712:Chlorides / Q000008:administration & dosage*; D003576:Electron Transport Complex IV / Q000037:antagonists & inhibitors / Q000378:metabolism*; D004789:Enzyme Activation / Q000187:drug effects / Q000502:physiology; D006624:Hippocampus / Q000187:drug effects / Q000201:enzymology; D057967:Infusions, Intraventricular; D008297:Male; D019001:Nitric Oxide Synthase / Q000096:biosynthesis / Q000378:metabolism*; D052247:Nitric Oxide Synthase Type II / Q000096:biosynthesis; D052250:Nitric Oxide Synthase Type III / Q000096:biosynthesis; D051381:Rats; D017208:Rats, Wistar; D015854:Up-Regulation / Q000187:drug effects / Q000502:physiology
chemical_list: D017607:Aluminum Compounds; D002712:Chlorides; D000077410:Aluminum Chloride; D019001:Nitric Oxide Synthase; D052247:Nitric Oxide Synthase Type II; D052250:Nitric Oxide Synthase Type III; C496318:Nos2 protein, rat; C496316:Nos3 protein, rat; D003576:Electron Transport Complex IV