title: The signal sequence of type II porcine reproductive and respiratory syndrome virus glycoprotein 3 is sufficient for endoplasmic reticulum retention.
abstract: The glycoprotein 3 (GP3) of type II porcine reproductive and respiratory syndrome virus has the characteristic domains of a membrane protein. However, this protein has been reported to be retained in the endoplasmic reticulum (ER) rather than transported to the plasma membrane of the cell. In this study, we performed confocal laser scanning microscopy analysis of variants of GP3 and found that the signal sequence of the GP3 led to confinement of GP3 in the ER, while the functional ortransmembrane domain did not affect its localization. Based on these results, we concluded that the signal sequence of GP3 contains the ER retention signal, which might play an important role in assembly of viral proteins.
authors.affiliation: Department of Infectious Disease, College of Veterinary Medicine, Konkuk University, Seoul 143-701, Korea.
authors.forename: Do-Geun
authors.initials: DG
authors.lastname: Kim
authors.forename: Chang-Seon
authors.initials: CS
authors.lastname: Song
authors.forename: In-Soo
authors.initials: IS
authors.lastname: Choi
authors.forename: Seung-Yong
authors.initials: SY
authors.lastname: Park
authors.forename: Joong-Bok
authors.initials: JB
authors.lastname: Lee
authors.forename: Sang-Soo
authors.initials: SS
authors.lastname: Lee
mesh_terms: D000818:Animals; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism* / Q000821:virology; D006224:Cricetinae; D004721:Endoplasmic Reticulum / Q000378:metabolism* / Q000821:virology; D018613:Microscopy, Confocal / Q000662:veterinary; D010957:Plasmids / Q000235:genetics / Q000378:metabolism; D019316:Porcine respiratory and reproductive syndrome virus / Q000235:genetics* / Q000378:metabolism; D021382:Protein Sorting Signals; D020539:Sequence Analysis, Protein / Q000662:veterinary; D014759:Viral Envelope Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism
chemical_list: D021382:Protein Sorting Signals; D014759:Viral Envelope Proteins
keywords: endoplasmic reticulum; glycoprotein 3; porcine reproductive and respiratory syndrome virus; retention signal; signal sequence