title: Propidium monoazide does not fully inhibit the detection of dead Campylobacter on broiler chicken carcasses by qPCR.
abstract: A real time quantitative PCR combined with propidium monoazide (PMA) treatment of samples was implemented to quantify live C. jejuni, C. coli and C. lari on broiler chicken carcasses at selected processing steps in the slaughterhouse. The samples were enumerated by culture for comparison. The Campylobacter counts determined with the PMA-qPCR and the culture method were not concordant. We conclude that the qPCR combined with PMA treatment of the samples did not fully reduce the signal from dead cells.
authors.affiliation: Division Veterinary Public Health, Institute for Risk Assessment Sciences, Utrecht University, The Netherlands.
authors.forename: Ewa
authors.initials: E
authors.lastname: Pacholewicz
authors.forename: Arno
authors.initials: A
authors.lastname: Swart
authors.forename: Len J A
authors.initials: LJ
authors.lastname: Lipman
authors.forename: Jaap A
authors.initials: JA
authors.lastname: Wagenaar
authors.forename: Arie H
authors.initials: AH
authors.lastname: Havelaar
authors.forename: Birgitta
authors.initials: B
authors.lastname: Duim
mesh_terms: D000818:Animals; D001386:Azides / Q000378:metabolism*; D058491:Bacterial Load / Q000379:methods*; D017000:Campylobacter coli / Q000302:isolation & purification* / Q000502:physiology; D016123:Campylobacter jejuni / Q000302:isolation & purification* / Q000502:physiology; D044863:Campylobacter lari / Q000302:isolation & purification* / Q000502:physiology; D002645:Chickens / Q000382:microbiology*; D050296:Microbial Viability; D011419:Propidium / Q000031:analogs & derivatives* / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*
chemical_list: D001386:Azides; C533957:propidium monoazide; D011419:Propidium
keywords: Broiler; Campylobacter; PMA; qPCR