title: Inhibitory activity of plant stilbenoids against nitric oxide production by lipopolysaccharide-activated microglia.
abstract: Microglia-driven inflammatory processes are thought to play an important role in ageing and several neurological disorders. Since consumption of a diet rich in polyphenols has been associated with anti-inflammatory and neuroprotective effects, we studied the effects of twenty-five stilbenoids isolated from Milicia excelsa, Morus alba, Gnetum africanum, and Vitis vinifera. These compounds were tested at 5 and 10 µM on BV-2 microglial cells stimulated with bacterial lipopolysaccharide. Ten stilbenoids reduced lipopolysaccharide-induced nitric oxide production at 5 and/or 10 µM. Two tetramers, E-vitisin A and E-vitisin B, were the most effective molecules. Moreover, they attenuated the expression of the inducible NO synthase protein and gene.
authors.affiliation: Groupe d'Etude des Substances Végétales à Activité Biologique, Université de Bordeaux, Institut des Sciences de la Vigne et du Vin, Villenave d'Ornon, France.
authors.forename: Merian
authors.initials: M
authors.lastname: Nassra
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Krisa
authors.forename: Yorgos
authors.initials: Y
authors.lastname: Papastamoulis
authors.forename: Gilbert Deccaux
authors.initials: GD
authors.lastname: Kapche
authors.forename: Jonathan
authors.initials: J
authors.lastname: Bisson
authors.forename: Caroline
authors.initials: C
authors.lastname: André
authors.forename: Jan-Pieter
authors.initials: JP
authors.lastname: Konsman
authors.forename: Jean-Marie
authors.initials: JM
authors.lastname: Schmitter
authors.forename: Jean-Michel
authors.initials: JM
authors.lastname: Mérillon
authors.forename: Pierre
authors.initials: P
authors.lastname: Waffo-Téguo
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000494:pharmacology*; D001572:Benzofurans / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D035684:Gnetum / Q000737:chemistry*; D008070:Lipopolysaccharides / Q000494:pharmacology; D017628:Microglia / Q000187:drug effects / Q000378:metabolism; D029586:Moraceae / Q000737:chemistry*; D031625:Morus / Q000737:chemistry*; D018696:Neuroprotective Agents / Q000737:chemistry / Q000494:pharmacology*; D009569:Nitric Oxide / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D010636:Phenols / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D018517:Plant Roots / Q000737:chemistry; D018547:Plant Stems / Q000737:chemistry; D059808:Polyphenols / Q000494:pharmacology; D013267:Stilbenes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D027843:Vitis / Q000737:chemistry*
chemical_list: D000893:Anti-Inflammatory Agents; D001572:Benzofurans; D008070:Lipopolysaccharides; D018696:Neuroprotective Agents; D010636:Phenols; D010936:Plant Extracts; D059808:Polyphenols; D013267:Stilbenes; C480486:malvidin-3-O-glucoside-4 vinyl; C412469:vitisin A; D009569:Nitric Oxide; C496319:NOS2 protein, human; D052247:Nitric Oxide Synthase Type II