title: The association of MDR1 C3435T and G2677T/A polymorphisms with plasma platelet-activating factor levels and coronary artery disease risk in Turkish population.
abstract: Increased levels of peripheral proinflammatory mediators can contribute to the development of coronary artery disease (CAD). Platelet activating factor (PAF) is an important proinflammatory mediator and plasma levels of PAF correlate with transmembrane transporter multidrug resistant 1 P-glycoprotein (MDR1 Pgp) expression and activity. MDR1 polymorphisms can affect the expression and activity of Pgp and plasma PAF levels. Therefore, we investigated the possible relationship between MDR1 C3435T and G2677T/A polymorphisms and plasma PAF levels and the risk of CAD. The study population consisted of 198 patients angiographically documented CAD, including 113 cases with at least 1 coronary artery with ≥50% luminal diameter stenosis and 85 control subjects with strictly normal coronary angiograms. Genotypes of the MDR1 C3435T and G2677T/A polymorphisms were determined by polymerase chain reaction (PCR) followed by restriction fragment length polymorphism (RFLP). Plasma PAF levels were detected by enzyme-linked immunosorbent assay (ELISA). There were no significant differences among plasma PAF levels in regard to MDR1 C3435T and G2677T polymorphisms in CAD patients and controls. No statistically significant difference was found for the genotypic and allelic distributions of the polymorphisms in the MDR1 gene between the patients and the control subjects. Furthermore, analysis of MDR1 haplotypes did not show any associations with increased plasma PAF levels and risk of CAD. Our results suggest that plasma PAF levels are not associated with MDR1 gene polymorphisms. There is no association between MDR1 C3435T and G2677T/A polymorphisms and the risk of CAD in Turkish patients.
authors.affiliation: Department of Medical Biology, Cerrahpasa Medical Faculty, University of Istanbul, Istanbul, Turkey.
authors.forename: Gulsel
authors.initials: G
authors.lastname: Ayaz
authors.forename: Bahadir
authors.initials: B
authors.lastname: Batar
authors.forename: Gonul
authors.initials: G
authors.lastname: Kanigur
authors.forename: Mehmet
authors.initials: M
authors.lastname: Guven
authors.forename: Ilhan
authors.initials: I
authors.lastname: Onaran
authors.forename: Bilgehan
authors.initials: B
authors.lastname: Karadag
authors.forename: Baris
authors.initials: B
authors.lastname: Ilerigelen
authors.forename: Ahmet
authors.initials: A
authors.lastname: Dirican
authors.forename: Turgut
authors.initials: T
authors.lastname: Ulutin
mesh_terms: D018435:ATP Binding Cassette Transporter, Subfamily B; D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000235:genetics*; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D016022:Case-Control Studies; D003324:Coronary Artery Disease / Q000097:blood / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D056726:Genetic Association Studies; D006239:Haplotypes; D006801:Humans; D008297:Male; D008875:Middle Aged; D015999:Multivariate Analysis; D010972:Platelet Activating Factor / Q000378:metabolism*; D020641:Polymorphism, Single Nucleotide; D012306:Risk; D014421:Turkey
chemical_list: C513055:ABCB1 protein, human; D018435:ATP Binding Cassette Transporter, Subfamily B; D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D010972:Platelet Activating Factor
keywords: CAD; Coronary artery disease; ELISA; MDR1; P-glycoprotein; P-gp; PAF; PCR; Platelet activating factor; Polymorphism; RFLP; coronary artery disease; enzyme-linked immunosorbent assay; multidrug-resistance 1; platelet activating factor; polymerase chain reaction; restriction fragment length polymorphism