title: Identification of calcium-transporting ATPases of Entamoeba histolytica and cellular localization of the putative SERCA.
abstract: Calcium has an important role on signaling of different cellular processes in the protozoa parasite Entamoeba histolytica, including development and pathogenesis. However, the systems that control calcium responses in this parasite are incompletely understood. Calcium-ATPases (Ca(2+)-ATPases) are proteins that play an important role in calcium homeostasis by catalyzing the active efflux of this ion from cytoplasm and are essential to the correct functioning of the cell machinery. Here, we reported the identification of five E. histolytica genes encoding putative Ca(2+)-ATPases, three related to PMCA, and two related to organellar ATPases. RT-PCR assays showed that all those genes are expressed in trophozoites and specific antibodies against the SERCA-like member located this protein in a continuous cytoplasmic network, supporting the hypothesis that it corresponds to the Ca(2+)-ATPase responsible to sequester calcium in the endoplasmic reticulum of this parasite.
authors.affiliation: Departamento de Infectómica y Patogénesis Molecular, Centro de Investigación y de Estudios Avanzados del IPN, A.P. 14-740, México D.F. 07000, Mexico.
authors.forename: Aarón
authors.initials: A
authors.lastname: Martinez-Higuera
authors.forename: Andrés
authors.initials: A
authors.lastname: Salas-Casas
authors.forename: Mercedes
authors.initials: M
authors.lastname: Calixto-Gálvez
authors.forename: Bibiana
authors.initials: B
authors.lastname: Chávez-Munguía
authors.forename: D Guillermo
authors.initials: DG
authors.lastname: Pérez-Ishiwara
authors.forename: Cecilia
authors.initials: C
authors.lastname: Ximénez
authors.forename: Mario A
authors.initials: MA
authors.lastname: Rodríguez
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002118:Calcium / Q000378:metabolism; D000252:Calcium-Transporting ATPases / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D004721:Endoplasmic Reticulum / Q000201:enzymology / Q000378:metabolism; D004748:Entamoeba histolytica / Q000737:chemistry / Q000201:enzymology* / Q000235:genetics; D005455:Fluorescent Antibody Technique; D018613:Microscopy, Confocal; D016253:Microscopy, Immunoelectron; D010802:Phylogeny; D011817:Rabbits; D053498:Sarcoplasmic Reticulum Calcium-Transporting ATPases / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D053498:Sarcoplasmic Reticulum Calcium-Transporting ATPases; D000252:Calcium-Transporting ATPases; D002118:Calcium
keywords: Calcium homeostasis; Calcium-ATPases; Endoplasmic reticulum; Entamoeba histolytica; Organellar ATPases