title: Aptamer-based plasmonic sensor array for discrimination of proteins and cells with the naked eye.
abstract: We developed a colorimetric sensor array with reported protein aptamers as nonspecific receptors. We found that different target proteins could make the aptamer-protected gold nanoparticles (AuNPs) exhibit different aggregation behaviors in the presence of a high concentration salt and cause various color change. On the basis of this phenomenon, we applied a series of reported protein aptamers as a receptor array obtaining a distinct response pattern to each target protein. Seven proteins have been well distinguished with the naked eye at the 50 nM level. Cancerous human cells have also been discriminated from noncancerous cells. This method is simple, label-free, and sensitive. It will broaden the application filed of plasmonic nanoparticle-based sensors and give a new direction of developing sensitive array sensing systems.
authors.forename: Yuexiang
authors.initials: Y
authors.lastname: Lu
authors.forename: Yueying
authors.initials: Y
authors.lastname: Liu
authors.forename: Suge
authors.initials: S
authors.lastname: Zhang
authors.forename: Song
authors.initials: S
authors.lastname: Wang
authors.forename: Sichun
authors.initials: S
authors.lastname: Zhang
authors.forename: Xinrong
authors.initials: X
authors.lastname: Zhang
mesh_terms: D052157:Aptamers, Nucleotide / Q000032:analysis*; D015374:Biosensing Techniques / Q000379:methods; D048430:Cell Shape; D003124:Colorimetry / Q000379:methods; D006801:Humans; D012709:Serum Albumin / Q000032:analysis*; D020349:Surface Plasmon Resonance / Q000379:methods*
chemical_list: D052157:Aptamers, Nucleotide; D012709:Serum Albumin