title: Non-specificity and synergy at the binding site of the carboplatin-induced DNA adduct via molecular dynamics simulations of the MutSα-DNA recognition complex.
abstract: MutSα is the most abundant mismatch-binding factor of human DNA mismatch repair (MMR) proteins. MMR maintains genetic stability by recognizing and repairing DNA defects. Failure to accomplish their function may lead to cancer. In addition, MutSα recognizes at least some types of DNA damage making it a target for anticancer agents. Here, complementing scarce experimental data, we report unique hydrogen-bonding motifs associated with the recognition of the carboplatin induced DNA damage by MutSα. These data predict that carboplatin and cisplatin induced damaging DNA adducts are recognized by MutSα in a similar manner. Our simulations also indicate that loss of base pairing at the damage site results in (1) non-specific binding and (2) changes in the atomic flexibility at the lesion site and beyond. To further quantify alterations at MutSα-DNA interface in response to damage recognition, non-bonding interactions and salt bridges were investigated. These data indicate (1) possible different packing and (2) disruption of the salt bridges at the MutSα-DNA interface in the damaged complex. These findings (1) underscore the general observation of disruptions at the MutSα-DNA interface and (2) highlight the nature of the anticancer effect of the carboplatin agent. The analysis was carried out from atomistic simulations.
authors.affiliation: a Department of Physics , Wake Forest University , Winston Salem , NC , 27106 , USA .
authors.forename: Lacramioara
authors.initials: L
authors.lastname: Negureanu
authors.forename: Freddie R
authors.initials: FR
authors.lastname: Salsbury
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry*; D001665:Binding Sites; D016190:Carboplatin / Q000737:chemistry*; D002945:Cisplatin / Q000737:chemistry; D018736:DNA Adducts / Q000737:chemistry*; D004249:DNA Damage; D053843:DNA Mismatch Repair; D006801:Humans; D006860:Hydrogen Bonding; D056004:Molecular Dynamics Simulation; D051719:MutS DNA Mismatch-Binding Protein / Q000737:chemistry*
chemical_list: D000970:Antineoplastic Agents; D018736:DNA Adducts; C096276:carboplatin-DNA adduct; D016190:Carboplatin; D051719:MutS DNA Mismatch-Binding Protein; D002945:Cisplatin