title: Micropatterning of mammalian cells on indium tin oxide substrates using ion implantation.
abstract: In this study, a simple surface patterning method to create micropatterns of mammalian cells on indium tin oxide (ITO) substrates was developed using ion implantation. Thin polystyrene (PS) films spin-coated on an ITO glass was selectively implanted with accelerated proton ions through a pattern mask and then developed to generate PS micropatterns. Well-organized negative PS patterns were generated on the ITO glass. The results of the in vitro cell culture on the PS-patterned ITO glass with two types of cancer cell lines revealed the formation of well-defined cell patterns through a selective cell adhesion and proliferation only onto the ITO regions separated by PS regions. This facile method for cell patterning may be used to create a desired platform for cellular device applications, such as biosensors and cell microarrays.
authors.affiliation: Research Division for Industry and Environment, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup-si, Jeollabuk-do 580-185, Republic of Korea.
authors.forename: In-Tae
authors.initials: IT
authors.lastname: Hwang
authors.forename: Mi-Young
authors.initials: MY
authors.lastname: Ahn
authors.forename: Chan-Hee
authors.initials: CH
authors.lastname: Jung
authors.forename: Jae-Hak
authors.initials: JH
authors.lastname: Choi
authors.forename: Kwanwoo
authors.initials: K
authors.lastname: Shin
mesh_terms: D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D018929:Cell Culture Techniques / Q000295:instrumentation / Q000379:methods; D048708:Cell Growth Processes / Q000187:drug effects / Q000502:physiology; D045744:Cell Line, Tumor; D020099:Coated Materials, Biocompatible / Q000138:chemical synthesis* / Q000737:chemistry; D005057:Eukaryotic Cells / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D006367:HeLa Cells; D006801:Humans; D007477:Ions / Q000737:chemistry*; D008422:Materials Testing; D013499:Surface Properties; D017971:Tin Compounds / Q000737:chemistry* / Q000494:pharmacology*; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D020099:Coated Materials, Biocompatible; D007477:Ions; D017971:Tin Compounds; C109984:indium tin oxide