title: Design and implementation of an imprinted material for the extraction of the endocrine disruptor bisphenol A from milk.
abstract: This paper describes the determination of bisphenol A (BPA) in milk samples, using a novel molecularly imprinted polymer. The imprinted polymer was developed using a rational design approach, and pre-polymerization interactions were investigated using molecular dynamics simulations and X-ray crystallography. A hydroquinone-imprinted polymer was used for solid phase extraction (SPE) clean-up of samples. BPA was quantified by high performance liquid chromatography (HPLC) and fluorescence (FLD) detection. Following validation, the method described was capable of determining bisphenol A in milk down to a limit of detection of 1.32μgkg(-1). The method was applied to a survey (n=27) of commercial milk products; BPA was detected in one of the samples, at a level of 176μgkg(-1). Test results were confirmed by a parallel UHPLC-MS/MS analytical method. This demonstrates the utility of the hydroquinone-imprinted polymer for application to selective sample clean-up and analysis of bisphenol A in milk, avoiding possible detrimental affects associated with template bleeding and without the need for expensive or difficult-to-obtain template.
authors.affiliation: Food Safety Department, Teagasc Food Research Centre, Ashtown, Dublin 15, Ireland. johnomahon2@gmail.com
authors.forename: John
authors.initials: J
authors.lastname: O'Mahony
authors.forename: Mary
authors.initials: M
authors.lastname: Moloney
authors.forename: Martin
authors.initials: M
authors.lastname: McCormack
authors.forename: Ian A
authors.initials: IA
authors.lastname: Nicholls
authors.forename: Boris
authors.initials: B
authors.lastname: Mizaikoff
authors.forename: Martin
authors.initials: M
authors.lastname: Danaher
mesh_terms: D000818:Animals; D001559:Benzhydryl Compounds / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D052244:Endocrine Disruptors / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*; D006873:Hydroquinones / Q000737:chemistry; D057230:Limit of Detection; D008892:Milk / Q000737:chemistry*; D054802:Molecular Imprinting; D010636:Phenols / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*; D011108:Polymers / Q000737:chemistry; D015203:Reproducibility of Results; D052616:Solid Phase Extraction; D053719:Tandem Mass Spectrometry; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D052244:Endocrine Disruptors; D006873:Hydroquinones; D010636:Phenols; D011108:Polymers; C006780:bisphenol A; D000098826:Bisphenol A Compounds; C031927:hydroquinone