title: Identification and characterization of P GCW14 : a novel, strong constitutive promoter of Pichia pastoris.
abstract: The available promoters in the Pichia pastoris expression platform are still limited. We selected and identified a novel strong constitutive promoter, P GCW14 , and tested its promoter activity using enhanced green fluorescent protein (EGFP) as a reporter. Potential promoter regions of P GCW14 were cloned upstream of the EGFP gene and promoter activity was analyzed by measuring fluorescence intensity. P GCW14 exhibited significantly stronger promoter activity than the classic strong constitutive promoters P TEF1 and P GAP under various carbon sources, suggesting that P GCW14 is a strong and constitutive promoter. Hence, P GCW14 can be used as a promoter for high-level expression of heterologous proteins.
authors.affiliation: School of Bioscience and Bioengineering, South China University of Technology, Guangzhou, 510006, Guangdong, China, liang.shuli@mail.scut.edu.cn.
authors.forename: Shuli
authors.initials: S
authors.lastname: Liang
authors.forename: Chengjuan
authors.initials: C
authors.lastname: Zou
authors.forename: Ying
authors.initials: Y
authors.lastname: Lin
authors.forename: Xuanwei
authors.initials: X
authors.lastname: Zhang
authors.forename: Yanrui
authors.initials: Y
authors.lastname: Ye
mesh_terms: D015870:Gene Expression; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000096:biosynthesis / Q000235:genetics; D010843:Pichia / Q000235:genetics*; D011401:Promoter Regions, Genetic; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics
chemical_list: D011994:Recombinant Proteins; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins