title: Effect of a recombinant manganese superoxide dismutase on prevention of contrast-induced acute kidney injury.
abstract: BACKGROUND
  Contrast media (CM)-induced nephropathy (CIN) is an acute deterioration of renal function following administration of CM mediated to a large extent by the increased production of ROS within the kidney. Aim of this study was to evaluate whether a novel isoform of a recombinant Manganese SOD (rMnSOD) could provide an effective protection against CIN; this molecule shares the same ability of physiological SODs in scavenging reactive oxygen species (ROS) but, due to its peculiar properties, enters inside the cells after its administration.
  
  
  METHODS
  We studied the effects rMnSOD on oxidative damage in a rat model of CIN in uninephrectomized rats, that were randomly assigned to 3 experimental Groups: Group CON, control rats treated with the vehicle of CM, Group HCM, rats treated with CM and Group SOD, rats treated with CM and rMnSOD.
  
  
  RESULTS
  In normal rats, pretreatment with rMnSOD, reduced renal superoxide anion production, induced by the activation of NAPDH oxidase, by 84 % (p < 0.001). In rats of Group HCM, ROS production was almost doubled compared to rat of Group CON (p < 0.01) but returned to normal values in rats of Group SOD, where a significant increase of SOD activity was detected (+16 % vs HCM, p < 0.05). Administration of CM determined a striking fall of GFR in rats of Group HCM (-70 %, p < 0.001 vs CON), greatly blunted in Group SOD (-28 % vs CON, p < 0.01); this was associated with a lower presence of both tubular necrosis and intratubular casts in SOD-treated rats (both p < 0.01 vs Group HCM).
  
  
  CONCLUSIONS
  Our data indicate that rMnSOD is able to reduce renal oxidative stress, thus preventing the reduction of GFR and the renal histologic damage that follows CM administration.
authors.affiliation: Department of Nephrology, University Federico II of Naples, 80131, Naples, Italy.
authors.forename: Antonio
authors.initials: A
authors.lastname: Pisani
authors.forename: Massimo
authors.initials: M
authors.lastname: Sabbatini
authors.forename: Eleonora
authors.initials: E
authors.lastname: Riccio
authors.forename: Roberta
authors.initials: R
authors.lastname: Rossano
authors.forename: Michele
authors.initials: M
authors.lastname: Andreucci
authors.forename: Clemente
authors.initials: C
authors.lastname: Capasso
authors.forename: Viviana
authors.initials: V
authors.lastname: De Luca
authors.forename: Vincenzo
authors.initials: V
authors.lastname: Carginale
authors.forename: Mariano
authors.initials: M
authors.lastname: Bizzarri
authors.forename: Antonella
authors.initials: A
authors.lastname: Borrelli
authors.forename: Antonella
authors.initials: A
authors.lastname: Schiattarella
authors.forename: Michele
authors.initials: M
authors.lastname: Santangelo
authors.forename: Aldo
authors.initials: A
authors.lastname: Mancini
mesh_terms: D058186:Acute Kidney Injury / Q000139:chemically induced* / Q000378:metabolism / Q000517:prevention & control*; D000818:Animals; D003287:Contrast Media / Q000009:adverse effects* / Q000494:pharmacology; D005919:Glomerular Filtration Rate / Q000187:drug effects / Q000502:physiology; D007668:Kidney / Q000187:drug effects / Q000378:metabolism / Q000503:physiopathology; D008297:Male; D023421:Models, Animal; D019255:NADPH Oxidases / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species / Q000378:metabolism; D011994:Recombinant Proteins / Q000494:pharmacology / Q000627:therapeutic use; D013482:Superoxide Dismutase / Q000494:pharmacology / Q000627:therapeutic use*
chemical_list: D003287:Contrast Media; D017382:Reactive Oxygen Species; D011994:Recombinant Proteins; D013482:Superoxide Dismutase; D019255:NADPH Oxidases