title: Anti-tumor effect of 4-Amino-2-Trifluoromethyl-Phenyl Retinate on human breast cancer MCF-7 cells via up-regulation of retinoid receptor-induced gene-1.
abstract: 4-Amino-2-Trifluoromethyl-Phenyl Retinate (ATPR) is one of the retinoid derivatives designed and synthesized in our team. In this paper, we explored the potential anti-tumor effects of ATPR in breast cancer. Here we found that ATPR showed remarkable anti-proliferative effects in a dose- and time-dependent manner, caused cell cycle arrest in the G0/G1 phase and significantly increased the expression of retinoid receptor-induced gene-1 (RRIG1). ATPR decreased the expression of phosphorylation-ERK (p-ERK) and increased the expression of estrogen receptor β (ERβ) and phosphorylation-p38 (p-p38). Following RRIG1 knockdown by RNAi interference, we found that the changes of ERβ, p-ERK and p-p38 induced by ATPR were both depressed. Our data suggest that ATPR could inhibit the proliferation and induce differentiation of MCF-7 cells via mediating the expression of RRIG1.
authors.affiliation: School of Pharmacy, Anhui Medical University, Mei Shan Road, Anhui Province, 230032 Hefei, PR China.
authors.forename: Nan
authors.initials: N
authors.lastname: Wang
authors.forename: Jin-Fang
authors.initials: JF
authors.lastname: Ge
authors.forename: Chun-Xiao
authors.initials: CX
authors.lastname: Pan
authors.forename: Xiao-Qing
authors.initials: XQ
authors.lastname: Peng
authors.forename: Hui-Hui
authors.initials: HH
authors.lastname: Chen
authors.forename: Xin-Qun
authors.initials: XQ
authors.lastname: Wang
authors.forename: Jie
authors.initials: J
authors.lastname: Tang
authors.forename: Wei
authors.initials: W
authors.lastname: Hu
authors.forename: Fei-Hu
authors.initials: FH
authors.lastname: Chen
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology*; D015153:Blotting, Western; D001943:Breast Neoplasms / Q000235:genetics / Q000473:pathology; D018929:Cell Culture Techniques; D059447:Cell Cycle Checkpoints / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D005260:Female; D006801:Humans; D061986:MCF-7 Cells; D008565:Membrane Proteins / Q000235:genetics*; D015394:Molecular Structure; D034741:RNA, Small Interfering / Q000235:genetics; D012176:Retinoids / Q000737:chemistry / Q000494:pharmacology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors; D025521:Tumor Suppressor Proteins / Q000235:genetics*; D015854:Up-Regulation
chemical_list: C585745:4-amino-2-trifluoromethyl-phenyl retinate; D000970:Antineoplastic Agents; D008565:Membrane Proteins; D034741:RNA, Small Interfering; C513407:RRIG1 protein, human; D012176:Retinoids; D025521:Tumor Suppressor Proteins
keywords: ATPR; Breast cancer cells; Estrogen receptors; MAPK pathway; RNA interference; Retinoid receptor-induced gene-1