title: Complete genome sequence of a novel Plum pox virus strain W isolate determined by 454 pyrosequencing.
abstract: The near-complete (99.7 %) genome sequence of a novel Russian Plum pox virus (PPV) isolate Pk, belonging to the strain Winona (W), has been determined by 454 pyrosequencing with the exception of the thirty-one 5'-terminal nucleotides. This region was amplified using 5'RACE kit and sequenced by the Sanger method. Genomic RNA released from immunocaptured PPV particles was employed for generation of cDNA library using TransPlex Whole transcriptome amplification kit (WTA2, Sigma-Aldrich). The entire Pk genome has identity level of 92.8-94.5 % when compared to the complete nucleotide sequences of other PPV-W isolates (W3174, LV-141pl, LV-145bt, and UKR 44189), confirming a high degree of variability within the PPV-W strain. The isolates Pk and LV-141pl are most closely related. The Pk has been found in a wild plum (Prunus domestica) in a new region of Russia indicating widespread dissemination of the PPV-W strain in the European part of the former USSR.
authors.affiliation: Department of Virology, Biological Faculty, Lomonosov Moscow State University, Leninskie Gory 1/12, Moscow, 119991, Russia.
authors.forename: Anna
authors.initials: A
authors.lastname: Sheveleva
authors.forename: Anna
authors.initials: A
authors.lastname: Kudryavtseva
authors.forename: Anna
authors.initials: A
authors.lastname: Speranskaya
authors.forename: Maxim
authors.initials: M
authors.lastname: Belenikin
authors.forename: Natalia
authors.initials: N
authors.lastname: Melnikova
authors.forename: Sergei
authors.initials: S
authors.lastname: Chirkov
mesh_terms: D016000:Cluster Analysis; D016679:Genome, Viral; D008969:Molecular Sequence Data; D010802:Phylogeny; D017801:Plum Pox Virus / Q000235:genetics* / Q000302:isolation & purification; D027861:Prunus / Q000821:virology; D012367:RNA, Viral / Q000235:genetics*; D012426:Russia; D017422:Sequence Analysis, DNA; D017385:Sequence Homology
chemical_list: D012367:RNA, Viral