title: Electrophoretic coating of amphiphilic chitosan colloids on regulating cellular behaviour.
abstract: In this communication, we report a facile nanotopographical control over a stainless steel surface via an electrophoretic deposition of colloidal amphiphilic chitosan for preferential growth, proliferation or migration of vascular smooth muscle cells (VSMCs) and human umbilical vein endothelial cells (HUVECs). Atomic force microscopy revealed that the colloidal surface exhibited a deposition time-dependent nanotopographical evolution, wherein two different nanotopographic textures indexed by 'kurtosis' (Rkur) value were easily designed, which were termed as 'sharp' (i.e. high peak-to-valley texture) surface and 'flat' (i.e. low peak-to-valley texture) surface. Cellular behaviour of VSMCs and HUVECs on both surfaces demonstrated topographically dependent morphogenesis, adherent responses and biochemical properties in comparison with bare stainless steel. The formation of a biofunctionalized surface upon a facile colloidal chitosan deposition envisions the potential application towards numerous biomedical devices, and this is especially promising for cardiovascular stents wherein a new surface with optimized texture can be designed and is expected to create an advantageous environment to stimulate HUVEC growth for improved healing performance.
authors.affiliation: Nano-Bioengineering Laboratory, Department of Materials Science and Engineering, National Chiao Tung University, No. 1001, Ta-Hseuh Road, Hsinchu City 300, Taiwan, Republic of China.
authors.forename: Yen-Jen
authors.initials: YJ
authors.lastname: Wang
authors.forename: Teng-Yuan
authors.initials: TY
authors.lastname: Lo
authors.forename: Chieh-Hsi
authors.initials: CH
authors.lastname: Wu
authors.forename: Dean-Mo
authors.initials: DM
authors.lastname: Liu
mesh_terms: D002465:Cell Movement; D049109:Cell Proliferation; D002478:Cells, Cultured; D048271:Chitosan / Q000737:chemistry*; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D003102:Colloids; D004586:Electrophoresis; D061307:Human Umbilical Vein Endothelial Cells / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000378:metabolism*; D032389:Myocytes, Smooth Muscle / Q000166:cytology / Q000378:metabolism*
chemical_list: D020099:Coated Materials, Biocompatible; D003102:Colloids; D048271:Chitosan
keywords: biomimetic materials; cell-specific growth; colloidal chitosan; electrophoretic deposition; nanotopographic surface