title: Transcriptomic profiling-based mutant screen reveals three new transcription factors mediating menadione resistance in Neurospora crassa.
abstract: To gain insight into the regulatory mechanisms of oxidative stress responses in filamentous fungi, the genome-wide transcriptional response of Neurospora crassa to menadione was analysed by digital gene expression (DGE) profiling, which identified 779 upregulated genes and 576 downregulated genes. Knockout mutants affecting 130 highly-upregulated genes were tested for menadione sensitivity, which revealed that loss of the transcription factor siderophore regulation (SRE) (a transcriptional repressor for siderophore biosynthesis), catatase-3, cytochrome c peroxidase or superoxide dismutase 1 copper chaperone causes hypersensitivity to menadione. Deletion of sre dramatically increased transcription of the siderophore biosynthesis gene ono and the siderophore iron transporter gene sit during menadione stress, suggesting that SRE is required for repression of iron uptake under oxidative stress conditions. Contrary to its phenotype, the sre deletion mutant showed higher transcriptional levels of genes encoding reactive oxygen species (ROS) scavengers than wild type during menadione stress, which implies that the mutant suffers a higher level of oxidative stress than wild type. Uncontrolled iron uptake in the sre mutant might exacerbate cellular oxidative stress. This is the first report of a negative regulator of iron assimilation participating in the fungal oxidative stress response. In addition to SRE, eight other transcription factor genes were also menadione-responsive but their single gene knockout mutants showed wild-type menadione sensitivity. Two of them, named as mit-2 (menadione induced transcription factor-2) and mit-4 (menadione induced transcription factor-4), were selected for double mutant analysis. The double mutant was hypersensitive to menadione. Similarly, the double mutation of mit-2 and sre also had additive effects on menadione sensitivity, suggesting multiple transcription factors mediate oxidative stress resistance in an additive manner.
authors.affiliation: State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, No. 8 BeiErTiao, ZhongGuanCun, Beijing 100080, China.
authors.forename: Jufen
authors.initials: J
authors.lastname: Zhu
authors.forename: Xinxu
authors.initials: X
authors.lastname: Yu
authors.forename: Baogui
authors.initials: B
authors.lastname: Xie
authors.forename: Xiaokui
authors.initials: X
authors.lastname: Gu
authors.forename: Zhenying
authors.initials: Z
authors.lastname: Zhang
authors.forename: Shaojie
authors.initials: S
authors.lastname: Li
mesh_terms: D025141:Drug Resistance, Fungal; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism; D020869:Gene Expression Profiling; D015966:Gene Expression Regulation, Fungal; D055786:Gene Knockout Techniques; D009492:Neurospora crassa / Q000187:drug effects* / Q000235:genetics*; D016877:Oxidants / Q000633:toxicity*; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism; D024483:Vitamin K 3 / Q000633:toxicity*
chemical_list: D005656:Fungal Proteins; D016877:Oxidants; D014157:Transcription Factors; D024483:Vitamin K 3