title: Towards lysozyme nanotube and 3D hybrid self-assembly.
abstract: We report lysozyme self-assembly into nanotubes, under the effect of hydrolysis at pH 2 and 90 °C. We resolve the final steps of the fibrillation pathway, entailing the closure of multi-stranded helical ribbons into nanotubes, and we provide evidence of β-sheet arrangement within the nanotubes, demonstrating amyloid-like aggregation. Addition of chloroauric acid to the self-assembled structures can lead to generation of either gold single crystal nanoplatelets or gold nanoparticles (when a reducing agent is added) decorating the nanotube and ribbon surfaces. The crystal-based organic-inorganic hybrids further assemble into 3D "sandwiched" structures.
authors.affiliation: Food and Soft Material Science, Institute of Food, Nutrition and Health, ETH Zürich, Schmelzbergstrasse 9, LFO, E23, 8092 Zürich, Switzerland.
authors.forename: Cecile
authors.initials: C
authors.lastname: Lara
authors.forename: Stephan
authors.initials: S
authors.lastname: Handschin
authors.forename: Raffaele
authors.initials: R
authors.lastname: Mezzenga
mesh_terms: D000682:Amyloid / Q000737:chemistry; D002712:Chlorides / Q000737:chemistry; D006046:Gold / Q000737:chemistry; D017612:Gold Compounds / Q000737:chemistry; D006868:Hydrolysis; D053768:Metal Nanoparticles / Q000737:chemistry; D009113:Muramidase / Q000737:chemistry* / Q000378:metabolism; D043942:Nanotubes / Q000737:chemistry*; D017433:Protein Structure, Secondary
chemical_list: D000682:Amyloid; D002712:Chlorides; D017612:Gold Compounds; D006046:Gold; C024568:gold tetrachloride, acid; D009113:Muramidase