title: Src Dependent Pancreatic Acinar Injury Can Be Initiated Independent of an Increase in Cytosolic Calcium.
abstract: Several deleterious intra-acinar phenomena are simultaneously triggered on initiating acute pancreatitis. These culminate in acinar injury or inflammatory mediator generation in vitro and parenchymal damage in vivo. Supraphysiologic caerulein is one such initiator which simultaneously activates numerous signaling pathways including non-receptor tyrosine kinases such as of the Src family. It also causes a sustained increase in cytosolic calcium- a player thought to be crucial in regulating deleterious phenomena. We have shown Src to be involved in caerulein induced actin remodeling, and caerulein induced changes in the Golgi and post-Golgi trafficking to be involved in trypsinogen activation, which initiates acinar cell injury. However, it remains unclear whether an increase in cytosolic calcium is necessary to initiate acinar injury or if injury can be initiated at basal cytosolic calcium levels by an alternate pathway. To study the interplay between tyrosine kinase signaling and calcium, we treated mouse pancreatic acinar cells with the tyrosine phosphatase inhibitor pervanadate. We studied the effect of the clinically used Src inhibitor Dasatinib (BMS-354825) on pervanadate or caerulein induced changes in Src activation, trypsinogen activation, cell injury, upstream cytosolic calcium, actin and Golgi morphology. Pervanadate, like supraphysiologic caerulein, induced Src activation, redistribution of the F-actin from its normal location in the sub-apical area to the basolateral areas, and caused antegrade fragmentation of the Golgi. These changes, like those induced by supraphysiologic caerulein, were associated with trypsinogen activation and acinar injury, all of which were prevented by Dasatinib. Interestingly, however, pervanadate did not cause an increase in cytosolic calcium, and the caerulein induced increase in cytosolic calcium was not affected by Dasatinib. These findings suggest that intra-acinar deleterious phenomena may be initiated independent of an increase in cytosolic calcium. Other players resulting in acinar injury along with the Src family of tyrosine kinases remain to be explored.
authors.affiliation: Department of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania, United States of America.
authors.forename: Vivek
authors.initials: V
authors.lastname: Mishra
authors.forename: Rachel
authors.initials: R
authors.lastname: Cline
authors.forename: Pawan
authors.initials: P
authors.lastname: Noel
authors.forename: Jenny
authors.initials: J
authors.lastname: Karlsson
authors.forename: Catherine J
authors.initials: CJ
authors.lastname: Baty
authors.forename: Lidiya
authors.initials: L
authors.lastname: Orlichenko
authors.forename: Krutika
authors.initials: K
authors.lastname: Patel
authors.forename: Ram Narayan
authors.initials: RN
authors.lastname: Trivedi
authors.forename: Sohail Z
authors.initials: SZ
authors.lastname: Husain
authors.forename: Chathur
authors.initials: C
authors.lastname: Acharya
authors.forename: Chandra
authors.initials: C
authors.lastname: Durgampudi
authors.forename: Donna B
authors.initials: DB
authors.lastname: Stolz
authors.forename: Sarah
authors.initials: S
authors.lastname: Navina
authors.forename: Vijay P
authors.initials: VP
authors.lastname: Singh
mesh_terms: D061354:Acinar Cells / Q000473:pathology*; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D003600:Cytosol / Q000378:metabolism*; D000069439:Dasatinib / Q000494:pharmacology; D004789:Enzyme Activation; D005455:Fluorescent Antibody Technique; D051379:Mice; D008813:Mice, Inbred ICR; D014638:Vanadates / Q000494:pharmacology; D019061:src-Family Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism*
chemical_list: C076715:pervanadate; D014638:Vanadates; D019061:src-Family Kinases; D000069439:Dasatinib; D002118:Calcium