title: Influenza virus A(H3N2) strain isolated from cerebrospinal fluid from a patient presenting myelopathy post infectious.
abstract: BACKGROUND
  Neurological involvement during influenza infection has been described during epidemics and is often consistent with serious sequelae or death.
  
  
  OBJECTIVE
  To investigate the etiologic agent involved in myelopathy post influenza-like syndrome.
  
  
  METHODS
  This investigation focuses on virus isolation from the cerebrospinal fluid (CSF) collected from a 19-year-old male student presenting with clinical diagnosis of myelopathy post influenza-like syndrome. To achieve this goal, different cell cultures and molecular methodologies were carried out.
  
  
  RESULTS
  Influenza virus A(H3N2) strain was isolated in MDCK cell culture; virus particles were observed under electron microscopy. Phylogenetics analyses showed that the Brazilian influenza A(H3N2) strains were closely related to the A/Perth/16/2009-like.
  
  
  CONCLUSIONS
  This study demonstrates that influenza virus A(H3N2) strain was the cause of illness of the students. According to the Brazilian influenza virus sentinel surveillance data A/Perth/16/2009-LIKE (H3N2) strain has predominated during the 2010 influenza virus season in Brasília-DF.
authors.affiliation: Núcleo de Doenças Respiratórias, Instituto Adolfo Lutz, São Paulo, SP, Brazil. tterezinha@uol.com.br
authors.forename: T M
authors.initials: TM
authors.lastname: Paiva
authors.forename: G
authors.initials: G
authors.lastname: Theotonio
authors.forename: R S
authors.initials: RS
authors.lastname: Paulino
authors.forename: M A
authors.initials: MA
authors.lastname: Benega
authors.forename: D B B
authors.initials: DB
authors.lastname: Silva
authors.forename: S E T
authors.initials: SE
authors.lastname: Borborema
authors.forename: T I
authors.initials: TI
authors.lastname: Ikeda
authors.forename: J J
authors.initials: JJ
authors.lastname: Kisielius
authors.forename: M
authors.initials: M
authors.lastname: Ueda
authors.forename: M I
authors.initials: MI
authors.lastname: Oliveira
authors.forename: C L S
authors.initials: CL
authors.lastname: Santos
mesh_terms: D000818:Animals; D001938:Brazil; D002460:Cell Line; D002555:Cerebrospinal Fluid / Q000821:virology*; D004285:Dogs; D006801:Humans; D053122:Influenza A Virus, H3N2 Subtype / Q000302:isolation & purification*; D007251:Influenza, Human / Q000150:complications* / Q000821:virology*; D008297:Male; D008854:Microscopy, Electron; D025202:Molecular Diagnostic Techniques; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D013118:Spinal Cord Diseases / Q000175:diagnosis* / Q000821:virology*; D014776:Virus Cultivation; D055815:Young Adult
keywords: Cerebrospinal fluid; Influenza A(H3N2) strain; Laboratory diagnosis; Real time RT-PCR; Virus sequencing