title: Heat transfer coefficient of cryotop during freezing.
abstract: Cryotop is an efficient vitrification method for cryopreservation of oocytes. It has been widely used owing to its simple operation and high freezing rate. Recently, the heat transfer performance of cryotop was studied by numerical simulation in several studies. However, the range of heat transfer coefficient in the simulation is uncertain. In this study, the heat transfer coefficient for cryotop during freezing process was analyzed. The cooling rates of 40 percent ethylene glycol (EG) droplet in cryotop during freezing were measured by ultra-fast measurement system and calculated by numerical simulation at different value of heat transfer coefficient. Compared with the results obtained by two methods, the range of the heat transfer coefficient necessary for the numerical simulation of cryotop was determined, which is between 9000 W/(m(2)·K) and 10000 W/(m (2)·K).
authors.affiliation: Institute of Biomedical Technology, University of Shanghai for Science and Technology, Shanghai, China.
authors.forename: W J
authors.initials: WJ
authors.lastname: Li
authors.forename: X L
authors.initials: XL
authors.lastname: Zhou
authors.forename: H S
authors.initials: HS
authors.lastname: Wang
authors.forename: B L
authors.initials: BL
authors.lastname: Liu
authors.forename: J J
authors.initials: JJ
authors.lastname: Dai
mesh_terms: D003198:Computer Simulation; D015925:Cryopreservation / Q000295:instrumentation*; D003451:Cryoprotective Agents / Q000737:chemistry*; D004867:Equipment Design; D019855:Ethylene Glycol / Q000737:chemistry*; D005615:Freezing; D006358:Hot Temperature; D008956:Models, Chemical; D058989:Vitrification
chemical_list: D003451:Cryoprotective Agents; D019855:Ethylene Glycol