title: A novel Au-nanoparticle biosensor for the rapid and simple detection of PSA using a sequence-specific peptide cleavage reaction.
abstract: PSA (prostate-specific antigen) is one of the most widely used proteins for the diagnosis of breast and prostate cancer. Of note, PSA displays enzymatic activity for the specific peptide sequence HSSKLQ, which it recognizes and cleaves. In this study, we developed a site-specific enzymatic-cleavage-reaction-based biosensor for the detection of PSA using fluorescein isothiocyanate (FITC)/peptide-conjugated gold (Au) nanoparticle complexes (FPANs). The FPANs do not initially fluoresce in the spectral region associated with the fluorophore, due to the quenching effect of the Au nanoparticles. When PSA was added to a solution containing the FPANs, PSA recognized and cleaved the specific sequence of the peptides attached to the Au nanoparticles. As a result, FITCs were separated from the Au nanoparticles and emitted strong fluorescence in their spectral region. Using this detection method, PSA was successfully detected as a function of concentration (10 pM-100 nM). This approach is superior to the immunoassay with respect to the performance of sensor, which is very rapid, simple, and one-step method for the detection of PSA and other protein markers can be measured for the early detection of several diseases.
authors.affiliation: Department of Chemical & Biomolecular Engineering, Sogang University, #1 Shinsu-Dong Mapo-Gu, Seoul 121-742, Republic of Korea.
authors.forename: Jin Ha
authors.initials: JH
authors.lastname: Choi
authors.forename: Hyun Soo
authors.initials: HS
authors.lastname: Kim
authors.forename: Jeong-Woo
authors.initials: JW
authors.lastname: Choi
authors.forename: Jong Wook
authors.initials: JW
authors.lastname: Hong
authors.forename: Young-Kee
authors.initials: YK
authors.lastname: Kim
authors.forename: Byung-Keun
authors.initials: BK
authors.lastname: Oh
mesh_terms: D000595:Amino Acid Sequence; D015374:Biosensing Techniques / Q000379:methods*; D016650:Fluorescein-5-isothiocyanate / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry; D006046:Gold / Q000737:chemistry*; D006801:Humans; D057230:Limit of Detection; D008969:Molecular Sequence Data; D053758:Nanoparticles / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry* / Q000378:metabolism; D017430:Prostate-Specific Antigen / Q000032:analysis* / Q000378:metabolism
chemical_list: D005456:Fluorescent Dyes; D010455:Peptides; D006046:Gold; D017430:Prostate-Specific Antigen; D016650:Fluorescein-5-isothiocyanate
keywords: Au nanoparticle; Enzymatic cleavage reaction; Nanobiosensor; Prostate specific antigen (PSA); Protein detection