title: Immunogenicity analysis of Staphylococcus aureus clumping factor A genetic variants.
abstract: The staphylococcal adhesin clumping factor A (ClfA) has a variant amino acid sequence, generating the potential for alterations in epitope structure and immunogenicity of this vaccine candidate. We demonstrated for two recombinant ClfA(40-531) (a slightly truncated version of the fibrinogen-binding domain of ClfA containing amino acids 40 to 531) genetic variants that strain-specific epitopes are immunodominant. This work indicates that immune responses elicited by ClfA may, at least in part, be dependent on the strain of origin of the ClfA.
authors.affiliation: Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, MD, USA.
authors.forename: Rebecca A
authors.initials: RA
authors.lastname: Brady
authors.forename: Christopher P
authors.initials: CP
authors.lastname: Mocca
authors.forename: Drusilla L
authors.initials: DL
authors.lastname: Burns
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000737:chemistry / Q000235:genetics* / Q000276:immunology*; D003030:Coagulase / Q000737:chemistry / Q000235:genetics* / Q000276:immunology*; D014644:Genetic Variation; D016056:Immunodominant Epitopes / Q000235:genetics*; D008807:Mice, Inbred BALB C; D008958:Models, Molecular; D011487:Protein Conformation; D013211:Staphylococcus aureus / Q000235:genetics* / Q000276:immunology*
chemical_list: D000942:Antigens, Bacterial; C515944:ClfA protein, Staphylococcus aureus; D003030:Coagulase; D016056:Immunodominant Epitopes