title: The role of transforming growth factor β1 in fractional laser resurfacing with a carbon dioxide laser.
abstract: The aim of this study was to investigate the role of transforming growth factor β1 in mechanisms of cutaneous remodeling induced by fractional carbon dioxide laser treatment. The dorsal skin of Kunming mice was exposed to a single-pass fractional CO2 laser treatment. Biopsies were taken at 1 h and at 1, 3, 7, 14, 21, 28, and 56 days after treatment. Transforming growth factor (TGF) β1 expression in skin samples was evaluated by ELISA, dermal thickness by hematoxylin-eosin staining, collagen and elastic fibers by Ponceau S and Victoria blue double staining, and types I and III collagens by ELISA. The level of TGF β1 in the laser-treated areas of skin was significantly increased compared with that in the control areas on days 1 (p < 0.05), 3 (p < 0.01), and 7 (p < 0.05) and then decreased by day 14 after treatment, at which time it had returned to the baseline level. Dermal thickness and the amount of type I collagen of the skin of the laser-treated areas had increased significantly (p < 0.05) compared with that in control areas on days 28 and 56. Fibroblast proliferation showed a positive correlation with TGF β1 expression during the early stages (r = 0.789, p < 0.01), and there was a negative correlation between the level of TGF β1 and type I collagen in the late stages, after laser treatment (r = -0.546, p < 0.05). TGF β1 appears to be an important factor in fractional laser resurfacing.
authors.affiliation: Institute for Laser Medicine and Bio-Photonics, Department of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, 200240, China.
authors.forename: Xia
authors.initials: X
authors.lastname: Jiang
authors.forename: Hongmei
authors.initials: H
authors.lastname: Ge
authors.forename: Chuanqing
authors.initials: C
authors.lastname: Zhou
authors.forename: Xinyu
authors.initials: X
authors.lastname: Chai
authors.forename: Hui
authors.initials: H
authors.lastname: Deng
mesh_terms: D000818:Animals; D049109:Cell Proliferation / Q000528:radiation effects; D024042:Collagen Type I / Q000378:metabolism; D005260:Female; D005347:Fibroblasts / Q000378:metabolism / Q000528:radiation effects; D054020:Lasers, Gas / Q000627:therapeutic use; D028022:Low-Level Light Therapy / Q000295:instrumentation / Q000379:methods*; D051379:Mice; D010789:Phototherapy / Q000379:methods; D012867:Skin / Q000378:metabolism* / Q000528:radiation effects; D053773:Transforming Growth Factor beta1 / Q000378:metabolism*; D014945:Wound Healing / Q000528:radiation effects
chemical_list: D024042:Collagen Type I; D053773:Transforming Growth Factor beta1