title: S100A6 competes with the TAZ2 domain of p300 for binding to p53 and attenuates p53 acetylation.
abstract: S100A6 is a calcium binding protein that, like some other members of the S100 protein family, is able to bind p53. This interaction may be physiologically relevant considering the numerous connotations of S100 proteins and of S100A6, in particular, with cancer and metastasis. In this work, we show that the interaction with S100A6 is limited to unmodified or phosphorylated p53 and is inhibited by p53 acetylation. Using in vitro acetylation assay, we show that the presence of S100A6 attenuates p53 acetylation by p300. Furthermore, using ELISA, we show that S100A6 and the TAZ2 domain of p300 bind p53 with similar affinities and that S100A6 effectively competes with TAZ2 for binding to p53. Our results add another element to the complicated scheme of p53 activation.
authors.affiliation: Nencki Institute of Experimental Biology, 3 Pasteur Street, 02-093 Warsaw, Poland.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Graczyk
authors.forename: Lukasz P
authors.initials: LP
authors.lastname: Słomnicki
authors.forename: Wiesława
authors.initials: W
authors.lastname: Leśniak
mesh_terms: D000107:Acetylation; D001667:Binding, Competitive / Q000502:physiology; D018797:Cell Cycle Proteins / Q000378:metabolism* / Q000502:physiology; D002478:Cells, Cultured; D015536:Down-Regulation; D050881:E1A-Associated p300 Protein / Q000737:chemistry* / Q000378:metabolism*; D006801:Humans; D008954:Models, Biological; D011485:Protein Binding; D054730:Protein Interaction Domains and Motifs / Q000502:physiology; D020033:Protein Isoforms / Q000378:metabolism; D000075383:S100 Calcium Binding Protein A6; D009418:S100 Proteins / Q000378:metabolism* / Q000502:physiology; D014157:Transcription Factors / Q000378:metabolism; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism*
chemical_list: D018797:Cell Cycle Proteins; D020033:Protein Isoforms; D000075383:S100 Calcium Binding Protein A6; D009418:S100 Proteins; C495901:TP53 protein, human; D014157:Transcription Factors; D016159:Tumor Suppressor Protein p53; C055351:S100A6 protein, human; D050881:E1A-Associated p300 Protein; C494360:EP300 protein, human
keywords: BSA; EGTA; PTM; RT; S100A6; TAZ2 domain; bovine serum albumin; ethylene glycol bis(β-aminoethyl ether) N,N′-tetraacetic acid; p300 acetyltransferase; p53; posttranslational modification; posttranslational modifications; room temperature