title: The combined effects of DEHP and PCBs on phospholipase in the livers of mice.
abstract: Di(2-ethylhexyl) phthalate (DEHP) and polychlorinated biphenyls (PCBs) are two widely distributed pollutants that are of great concern due to their adverse health effects. However, few studies have investigated the combined effects of DEHP and PCBs. In this study, adult mice were continuously exposed to mixtures of DEHP (15 mg/kg bodyweight/day) and Aroclor 1254 (7.5 mg/kg bodyweight/day) for 12 days to investigate the combined effects of these compounds. The results showed that the ratio of the liver weight to the body weight was higher in the treated group than that in the control group. The effects of combined exposure on three important receptors, the proliferator-activated receptor (PPAR), estrogen receptor (ER), and aryl hydrocarbon receptor (AHR), were investigated. The mRNA level of PPARγ was significantly up-regulated after exposure. The expression level of ERα was decreased in the male treated group. In contrast, the expression levels of AHR and related genes (cyp1a1 and cyp1b1) were not markedly affected. The expression level of phospholipase A (PLA) was significantly down-regulated at both the mRNA and protein levels in male mice after combined treatment. In all, our study demonstrated the combined effects of DEHP and PCBs on the expression levels of key receptors in mice. The combined exposure led to a decrease in phospholipase in male mice.
authors.affiliation: Key Laboratory of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen, 361021, People's Republic of China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Lin
authors.forename: Lingli
authors.initials: L
authors.lastname: Min
authors.forename: Qiansheng
authors.initials: Q
authors.lastname: Huang
authors.forename: Yajie
authors.initials: Y
authors.lastname: Chen
authors.forename: Chao
authors.initials: C
authors.lastname: Fang
authors.forename: Xia
authors.initials: X
authors.lastname: Sun
authors.forename: Sijun
authors.initials: S
authors.lastname: Dong
mesh_terms: D000818:Animals; D001835:Body Weight / Q000187:drug effects; D020111:Chlorodiphenyl (54% Chlorine) / Q000633:toxicity; D019363:Cytochrome P-450 CYP1A1 / Q000096:biosynthesis; D065633:Cytochrome P-450 CYP1B1 / Q000096:biosynthesis; D004051:Diethylhexyl Phthalate / Q000633:toxicity*; D004347:Drug Interactions; D004785:Environmental Pollutants / Q000633:toxicity*; D005260:Female; D008099:Liver / Q000187:drug effects / Q000201:enzymology*; D008297:Male; D051379:Mice; D009929:Organ Size / Q000187:drug effects; D047495:PPAR gamma / Q000378:metabolism; D010740:Phospholipases / Q000378:metabolism*; D011078:Polychlorinated Biphenyls / Q000633:toxicity*; D018336:Receptors, Aryl Hydrocarbon / Q000187:drug effects; D011960:Receptors, Estrogen / Q000378:metabolism
chemical_list: D004785:Environmental Pollutants; D047495:PPAR gamma; D018336:Receptors, Aryl Hydrocarbon; D011960:Receptors, Estrogen; D020111:Chlorodiphenyl (54% Chlorine); D004051:Diethylhexyl Phthalate; D011078:Polychlorinated Biphenyls; C585498:Cyp1b1 protein, mouse; D019363:Cytochrome P-450 CYP1A1; D065633:Cytochrome P-450 CYP1B1; D010740:Phospholipases
keywords: DEHP; PPAR; aroclor 1254; phospholipase; receptor