title: [Effect of electroacupuncture on expression of myocardial PI 3 K, HIF-1alpha and VEGF in rats with cerebral-cardiac syndrome].
abstract: OBJECTIVE
  To observe the effect of electroacupuncture (EA) on the expression of myocardial 1-phosphatidylinositol 3-kinase (PI 3 K), hypoxia-inducible factor-1alpha (HIF-1alpha) and vascular endothelial growth factor (VEGF) in rats with cerebral-cardiac syndrome (CCS), so as to reveal its mechanism underlying reducing ischemic myocardial injury.
  
  
  METHODS
  Forty SD rats were randomly and equally divided into sham-operation, model, EA and non-acupoint (the lateral-superior side of the hip) groups (10 rats/group). CCS model was established by injection of collagenase (1 U/microL) and heparin (7 U/microL) into the right caudate nucleus. Following modeling, EA (1.5 mA, 2 Hz, 20 min) was applied to "Shuigou" (GV 26), "Fengfu" (GV 16), "Neiguan" (PC 6) and "Xinshu" (BL 15) acupoints, once daily for three consecutive days. The expression levels of PI 3 K,HIF-1alpha and VEGF in the myocardium were detected by immunohistochemistry.
  
  
  RESULTS
  Compared with the sham-operation group, the expression levels of myocardial PI 3 K, HIF-1a and VEGF proteins were significantly increased in the model group (P<0.01). While in comparison with the model group, there were little increase in the non-acupoint group (P>0.05) and considerable increase in the expression levels of the 3 myocardial proteins in the EA group (P<0.05).
  
  
  CONCLUSIONS
  EA intervention has a function in upregulating the expression of myocardial VEGF, HIF-1alpha and PI 3 K proteins in CCS rats, which maybe contribute to its protective effect on ischaemic myocardial injury.
authors.forename: Sheng-Bing
authors.initials: SB
authors.lastname: Wu
authors.forename: Jian
authors.initials: J
authors.lastname: Cao
authors.forename: Fang
authors.initials: F
authors.lastname: Gao
authors.forename: Li-Na
authors.initials: LN
authors.lastname: Wang
authors.forename: Meng-Juan
authors.initials: MJ
authors.lastname: Chang
authors.forename: Jing-Jing
authors.initials: JJ
authors.lastname: Xue
authors.forename: Tian-Ning
authors.initials: TN
authors.lastname: Zhang
authors.forename: Mei-Qi
authors.initials: MQ
authors.lastname: Zhou
mesh_terms: D015669:Acupuncture Points; D000818:Animals; D002526:Cerebellar Diseases / Q000235:genetics* / Q000378:metabolism / Q000628:therapy*; D015671:Electroacupuncture; D005260:Female; D006331:Heart Diseases / Q000235:genetics* / Q000378:metabolism / Q000628:therapy*; D006801:Humans; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000235:genetics* / Q000378:metabolism; D008297:Male; D058539:Phosphatidylinositol 3-Kinase / Q000235:genetics* / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics* / Q000378:metabolism
chemical_list: D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D042461:Vascular Endothelial Growth Factor A; D058539:Phosphatidylinositol 3-Kinase