title: Benzochloroporphyrin derivative induced cytotoxicity and inhibition of tumor recurrence during photodynamic therapy for osteosarcoma.
abstract: Photodynamic therapy (PDT) is a promising cancer treatment modality that uses dye-sensitized photooxidation of biologic matter in target tissue. This study explored effects of the photosensitizer BCPD-17 during PDT for osteosarcoma. LM-8 osteosarcoma cells were treated with BCPD-17 and cell viability after laser irradiation was assessed in vitro with the 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide assay. The effects of BCPD-17 during PDT recurrence were then examined on tumor-bearing mice in vivo. BCPD-17 had dose- dependent cytotoxic effects on LM-8 osteosarcoma cells after laser irradiation which also had energy-dependent effects on the cells. The rate of local recurrence was reduced when marginal resection of mice tumors was followed by BCPD-17-mediated PDT. Our results indicated BCPD-17-mediated PDT in combination with marginal resection of tumors is a potentially new effective treatment for osteosarcoma.
authors.affiliation: Musculoskeletal Oncology Center, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai, China.
authors.forename: Hai-Yang
authors.initials: HY
authors.lastname: Gong
authors.forename: Meng-Xiong
authors.initials: MX
authors.lastname: Sun
authors.forename: Shuo
authors.initials: S
authors.lastname: Hu
authors.forename: Ying-Ying
authors.initials: YY
authors.lastname: Tao
authors.forename: Bo
authors.initials: B
authors.lastname: Gao
authors.forename: Guo-Dong
authors.initials: GD
authors.lastname: Li
authors.forename: Zheng-Dong
authors.initials: ZD
authors.lastname: Cai
authors.forename: Jian-Zhong
authors.initials: JZ
authors.lastname: Yao
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D001859:Bone Neoplasms / Q000188:drug therapy; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D005260:Female; D051379:Mice; D008809:Mice, Inbred C3H; D008819:Mice, Nude; D009364:Neoplasm Recurrence, Local / Q000188:drug therapy* / Q000517:prevention & control; D012516:Osteosarcoma / Q000188:drug therapy* / Q000601:surgery; D010778:Photochemotherapy; D017319:Photosensitizing Agents / Q000737:chemistry / Q000494:pharmacology* / Q000633:toxicity; D011166:Porphyrins / Q000737:chemistry / Q000494:pharmacology* / Q000633:toxicity
chemical_list: D017319:Photosensitizing Agents; D011166:Porphyrins