title: Cell geometric constraints induce modular gene-expression patterns via redistribution of HDAC3 regulated by actomyosin contractility.
abstract: Physical forces in the form of substrate rigidity or geometrical constraints have been shown to alter gene expression profile and differentiation programs. However, the underlying mechanism of gene regulation by these mechanical cues is largely unknown. In this work, we use micropatterned substrates to alter cellular geometry (shape, aspect ratio, and size) and study the nuclear mechanotransduction to regulate gene expression. Genome-wide transcriptome analysis revealed cell geometry-dependent alterations in actin-related gene expression. Increase in cell size reinforced expression of matrix-related genes, whereas reduced cell-substrate contact resulted in up-regulation of genes involved in cellular homeostasis. We also show that large-scale changes in gene-expression profile mapped onto differential modulation of nuclear morphology, actomyosin contractility and histone acetylation. Interestingly, cytoplasmic-to-nuclear redistribution of histone deacetylase 3 modulated histone acetylation in an actomyosin-dependent manner. In addition, we show that geometric constraints altered the nuclear fraction of myocardin-related transcription factor. These fractions exhibited hindered diffusion time scale within the nucleus, correlated with enhanced serum-response element promoter activity. Furthermore, nuclear accumulation of myocardin-related transcription factor also modulated NF-κB activity. Taken together, our work provides modularity in switching gene-expression patterns by cell geometric constraints via actomyosin contractility.
authors.affiliation: Mechanobiology Institute, National University of Singapore, Singapore 117411.
authors.forename: Nikhil
authors.initials: N
authors.lastname: Jain
authors.forename: K Venkatesan
authors.initials: KV
authors.lastname: Iyer
authors.forename: Abhishek
authors.initials: A
authors.lastname: Kumar
authors.forename: G V
authors.initials: GV
authors.lastname: Shivashankar
mesh_terms: D016475:3T3 Cells; D000107:Acetylation; D000205:Actomyosin / Q000502:physiology*; D000818:Animals; D005786:Gene Expression Regulation; D006655:Histone Deacetylases / Q000378:metabolism*; D006657:Histones / Q000378:metabolism; D051379:Mice; D014158:Transcription, Genetic; D000099186:Histone Deacetylase 3
chemical_list: D000205:Actomyosin; D006655:Histone Deacetylases; D006657:Histones; D000099186:Histone Deacetylase 3
keywords: MRTF-A signaling; cell matrix interaction; chromatin remodelling; substrate geometry; transcription control