title: The million mutation project: a new approach to genetics in Caenorhabditis elegans.
abstract: We have created a library of 2007 mutagenized Caenorhabditis elegans strains, each sequenced to a target depth of 15-fold coverage, to provide the research community with mutant alleles for each of the worm's more than 20,000 genes. The library contains over 800,000 unique single nucleotide variants (SNVs) with an average of eight nonsynonymous changes per gene and more than 16,000 insertion/deletion (indel) and copy number changes, providing an unprecedented genetic resource for this multicellular organism. To supplement this collection, we also sequenced 40 wild isolates, identifying more than 630,000 unique SNVs and 220,000 indels. Comparison of the two sets demonstrates that the mutant collection has a much richer array of both nonsense and missense mutations than the wild isolate set. We also find a wide range of rDNA and telomere repeat copy number in both sets. Scanning the mutant collection for molecular phenotypes reveals a nonsense suppressor as well as strains with higher levels of indels that harbor mutations in DNA repair genes and strains with abundant males associated with him mutations. All the strains are available through the Caenorhabditis Genetics Center and all the sequence changes have been deposited in WormBase and are available through an interactive website.
authors.affiliation: Department of Genome Sciences, University of Washington, Seattle, Washington 98195, USA;
authors.forename: Owen
authors.initials: O
authors.lastname: Thompson
authors.forename: Mark
authors.initials: M
authors.lastname: Edgley
authors.forename: Pnina
authors.initials: P
authors.lastname: Strasbourger
authors.forename: Stephane
authors.initials: S
authors.lastname: Flibotte
authors.forename: Brent
authors.initials: B
authors.lastname: Ewing
authors.forename: Ryan
authors.initials: R
authors.lastname: Adair
authors.forename: Vinci
authors.initials: V
authors.lastname: Au
authors.forename: Iasha
authors.initials: I
authors.lastname: Chaudhry
authors.forename: Lisa
authors.initials: L
authors.lastname: Fernando
authors.forename: Harald
authors.initials: H
authors.lastname: Hutter
authors.forename: Armelle
authors.initials: A
authors.lastname: Kieffer
authors.forename: Joanne
authors.initials: J
authors.lastname: Lau
authors.forename: Norris
authors.initials: N
authors.lastname: Lee
authors.forename: Angela
authors.initials: A
authors.lastname: Miller
authors.forename: Greta
authors.initials: G
authors.lastname: Raymant
authors.forename: Bin
authors.initials: B
authors.lastname: Shen
authors.forename: Jay
authors.initials: J
authors.lastname: Shendure
authors.forename: Jon
authors.initials: J
authors.lastname: Taylor
authors.forename: Emily H
authors.initials: EH
authors.lastname: Turner
authors.forename: LaDeana W
authors.initials: LW
authors.lastname: Hillier
authors.forename: Donald G
authors.initials: DG
authors.lastname: Moerman
authors.forename: Robert H
authors.initials: RH
authors.lastname: Waterston
mesh_terms: D000483:Alleles; D000818:Animals; D017173:Caenorhabditis elegans / Q000145:classification / Q000235:genetics*; D018389:Codon, Nonsense; D056915:DNA Copy Number Variations; D004275:DNA, Ribosomal; D030561:Databases, Nucleic Acid; D020043:Genes, Essential; D017238:Genes, Helminth; D016153:Genes, Suppressor; D014644:Genetic Variation; D049751:Genome, Helminth; D054629:Genome, Mitochondrial; D006579:Heterozygote; D054643:INDEL Mutation; D008297:Male; D009154:Mutation; D020125:Mutation, Missense; D010641:Phenotype; D020641:Polymorphism, Single Nucleotide; D020080:Tandem Repeat Sequences
chemical_list: D018389:Codon, Nonsense; D004275:DNA, Ribosomal