title: Resveratrol induces autophagy through death-associated protein kinase 1 (DAPK1) in human dermal fibroblasts under normal culture conditions.
abstract: Autophagy is an essential process degrading damaged components. Although resveratrol has various beneficial activities for health, little is known about the effects of resveratrol on autophagy in skin. We investigated whether resveratrol affects autophagy in human dermal fibroblasts grown in complete medium. We found that after the resveratrol treatment, LC3-II reached a maximum level at 8 h and then gradually decreased. By PCR array analysis, we identified death-associated protein kinase 1 (DAPK1) as a new target of resveratrol, and we confirmed that the expression level of DAPK1 was enhanced by resveratrol. We also demonstrated that DAPK1 knock-down by siRNA was sufficient to reduce resveratrol-induced autophagy but did not affect the phosphorylation level of AMP-activated kinase (AMPK), a well-known target of resveratrol. These data indicate that resveratrol-induced autophagy can be mediated by DAPK1, raising the possibility that some of the beneficial effects of resveratrol may be due to its regulation of DAPK1.
authors.forename: Min Sik
authors.initials: MS
authors.lastname: Choi
authors.forename: Yoonkyung
authors.initials: Y
authors.lastname: Kim
authors.forename: Ji-Yong
authors.initials: JY
authors.lastname: Jung
authors.forename: Seung Ha
authors.initials: SH
authors.lastname: Yang
authors.forename: Tae Ryong
authors.initials: TR
authors.lastname: Lee
authors.forename: Dong Wook
authors.initials: DW
authors.lastname: Shin
mesh_terms: D055372:AMP-Activated Protein Kinases / Q000378:metabolism; D001343:Autophagy; D018929:Cell Culture Techniques; D064550:Death-Associated Protein Kinases / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D004789:Enzyme Activation; D004791:Enzyme Inhibitors / Q000737:chemistry; D005347:Fibroblasts / Q000378:metabolism*; D006801:Humans; D008247:Lysosomes / Q000378:metabolism; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D010766:Phosphorylation; D016133:Polymerase Chain Reaction; D034741:RNA, Small Interfering / Q000378:metabolism; D000077185:Resveratrol; D012867:Skin / Q000166:cytology*; D013267:Stilbenes / Q000737:chemistry*; D013997:Time Factors
chemical_list: D004791:Enzyme Inhibitors; C477012:MAP1LC3A protein, human; D008869:Microtubule-Associated Proteins; D034741:RNA, Small Interfering; D013267:Stilbenes; C579096:DAPK1 protein, human; D064550:Death-Associated Protein Kinases; D055372:AMP-Activated Protein Kinases; D000077185:Resveratrol