title: Complete genome sequence of a novel porcine parainfluenza virus 5 isolate in Korea.
abstract: A novel cytopathogenic paramyxovirus was isolated from a lung sample from a piglet, using continuous porcine alveolar macrophage cells. Morphologic and genetic studies indicated that this porcine virus (pPIV5) belongs to the species Parainfluenza 5 in the family Paramyxoviridae. We attempted to determine the complete nucleotide sequence of the first Korean pPIV5 isolate, designated KNU-11. The full-length genome of KNU-11 was found to be 15,246 nucleotides in length and consist of seven nonoverlapping genes (3'-N-V/P-M-F-SH-HN-L-5') predicted to encode eight proteins. The overall degree of nucleotide sequence identity was 98.7 % between KNU-11 and PIV5 (formerly simian virus 5, SV5), a prototype paramyxovirus, and the putative proteins had 74.4 to 99.2 % amino acid identity to those of PIV5. Phylogenetic analysis further demonstrated that the novel pPIV5 isolate is a member of the genus Rubulavirus of the subfamily Paramyxovirinae. The present study describes the identification and genomic characterization of a pPIV5 isolate in South Korea.
authors.affiliation: Department of Microbiology, College of Natural Sciences, Kyungpook National University, Daegu, 702-701, Republic of Korea.
authors.forename: Yu Na
authors.initials: YN
authors.lastname: Lee
authors.forename: Changhee
authors.initials: C
authors.lastname: Lee
mesh_terms: D000818:Animals; D016000:Cluster Analysis; D016679:Genome, Viral; D008168:Lung / Q000821:virology; D008969:Molecular Sequence Data; D018184:Paramyxoviridae Infections / Q000662:veterinary / Q000821:virology; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics*; D056910:Republic of Korea; D019213:Rubulavirus / Q000235:genetics* / Q000302:isolation & purification; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D013552:Swine; D013553:Swine Diseases / Q000821:virology
chemical_list: D012367:RNA, Viral