title: Lipase production by diverse phylogenetic clades of Aureobasidium pullulans.
abstract: Thirty-nine strains representing 12 diverse phylogenetic clades of Aureobasidium pullulans were surveyed for lipase production using a quantitative assay. Strains in clades 4 and 10 produced 0.2-0.3 U lipase/ml, while color variant strain NRRL Y-2311-1 in clade 8 produced 0.54 U lipase/ml. Strains in clade 9, which exhibit a dark olivaceous pigment, produced the highest levels of lipase, with strain NRRL 62034 yielding 0.57 U lipase/ml. By comparison, Candida cylindracea strain NRRL Y-17506 produced 0.05 U lipase/ml under identical conditions. A. pullulans strain NRRL 62034 reached maximal lipase levels in 5 days on lipase induction medium, while A. pullulans strain NRRL Y-2311-1 and strains in clades 4 and 10 were highest after 6 days. A. pullulans strain NRRL Y-2311-1 and strains in clade 9 produced two extracellular proteins in common, at >50 and <37 kDa.
authors.affiliation: Renewable Product Technology Research Unit National Center for Agricultural Utilization Research, Agricultural Research Service, U.S. Department of Agriculture, 1815 North University Street, Peoria, IL, 61604, USA, Tim.Leathers@ars.usda.gov.
authors.forename: Timothy D
authors.initials: TD
authors.lastname: Leathers
authors.forename: Joseph O
authors.initials: JO
authors.lastname: Rich
authors.forename: Amber M
authors.initials: AM
authors.lastname: Anderson
authors.forename: Pennapa
authors.initials: P
authors.lastname: Manitchotpisit
mesh_terms: D001203:Ascomycota / Q000201:enzymology*; D002175:Candida / Q000201:enzymology; D003470:Culture Media / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D008049:Lipase / Q000032:analysis* / Q000737:chemistry; D008970:Molecular Weight; D013997:Time Factors
chemical_list: D003470:Culture Media; D008049:Lipase