title: Complementary laboratory indices for predicting the disease status of patients with hepatitis B virus infection.
abstract: To identify complementary laboratory indices for determining the disease status of patients with hepatitis B virus. Subjects were divided into six groups: hepatitis B virus carrier, mild chronic hepatitis B, moderate chronic hepatitis B, severe chronic hepatitis B, fulminant hepatitis B and healthy controls. Serum alanine aminotransferase, total bilirubin and direct bilirubin were measured by an automatic analyser. The levels of T-cell immunoglobulin domain and mucin-domain-containing molecule-3, macrophage inflammatory protein 2, neutrophil gelatinase-associated lipocalin and inducible nitric oxide synthase were measured by ELISA. T-cell immunoglobulin domain, mucin-domain-containing molecule-3, macrophage inflammatory protein 2 and inducible nitric oxide synthase levels were significantly higher in patients with severe chronic hepatitis B compared with those in patients with mild and moderate chronic hepatitis B or fulminant hepatitis B (P < 0.05). When normal or abnormal alanine aminotransferase was present, significant differences between macrophage inflammatory protein 2 and T-cell immunoglobulin domain and mucin-domain-containing molecule-3 levels between patients with mild, moderate, severe chronic hepatitis B or fulminant hepatitis B were observed (P < 0.05). Our results suggest that T-cell immunoglobulin domain and mucin-domain-containing molecule-3 and macrophage inflammatory protein 2 could serve as alanine aminotransferase, direct bilirubin or total bilirubin complementary indices for determining the status of patients with hepatitis B.
authors.affiliation: State Key Laboratory of Infectious Disease Diagnosis and Treatment, First Affiliated Hospital, College of Medicine, Zhejiang University, Key Laboratory of Infectious Diseases, Zhejiang, China.
authors.forename: F
authors.initials: F
authors.lastname: Chen
authors.forename: J L
authors.initials: JL
authors.lastname: He
authors.forename: M
authors.initials: M
authors.lastname: Zheng
authors.forename: H H
authors.initials: HH
authors.lastname: Zhu
authors.forename: S P
authors.initials: SP
authors.lastname: Li
authors.forename: K
authors.initials: K
authors.lastname: Wang
authors.forename: X X
authors.initials: XX
authors.lastname: Zhang
authors.forename: Y R
authors.initials: YR
authors.lastname: Zhao
authors.forename: S S
authors.initials: SS
authors.lastname: Wu
authors.forename: Z
authors.initials: Z
authors.lastname: Chen
mesh_terms: D000328:Adult; D000410:Alanine Transaminase / Q000097:blood; D001663:Bilirubin / Q000097:blood; D015415:Biomarkers / Q000097:blood*; D016207:Cytokines / Q000097:blood; D003955:Diagnostic Tests, Routine / Q000379:methods*; D005260:Female; D006509:Hepatitis B / Q000175:diagnosis* / Q000473:pathology*; D006801:Humans; D008111:Liver Function Tests / Q000379:methods*; D008297:Male; D008875:Middle Aged; D012720:Severity of Illness Index; D055815:Young Adult
chemical_list: D015415:Biomarkers; D016207:Cytokines; D000410:Alanine Transaminase; D001663:Bilirubin
keywords: T-cell immunoglobulin domain and mucin-domain-containing molecule-3; hepatitis B virus infection; inducible nitric oxide synthase; macrophage inflammatory protein 2; neutrophil gelatinase-associated lipocalin