title: Emergence of VIM-4 metallo-β-lactamase-producing Klebsiella pneumoniae ST15 clone in the Clinical Centre University of Pécs, Hungary.
abstract: Since November 2009 carbapenemase-producing Klebsiella pneumoniae isolates have been detected in increasing numbers at the Clinical Centre University of Pécs. Molecular typing was performed for 102 clinical isolates originating from different time periods and various departments of the Clinical Centre. Pulsed-field gel electrophoresis revealed the predominance of a single clone (101/102), identified as sequence type ST15. PCR and sequencing showed the presence of blaCTX-M-15 and blaVIM-4 genes. The blaVIM-4 was located on a class 1 integron designated In238b. To our knowledge, this is the first description of a blaVIM-4 gene in the predominant CTX-M-15 extended spectrum β-lactamase-producing Hungarian Epidemic Clone/ST15.
authors.affiliation: Department of Medical Microbiology and Immunology, Medical School, University of Pécs, Pécs, Hungary; Department of Diagnostic Laboratory, János Balassa Hospital, County Tolna, Szekszárd, Hungary.
authors.forename: S
authors.initials: S
authors.lastname: Melegh
authors.forename: K
authors.initials: K
authors.lastname: Kovács
authors.forename: T
authors.initials: T
authors.lastname: Gám
authors.forename: A
authors.initials: A
authors.lastname: Nyul
authors.forename: B
authors.initials: B
authors.lastname: Patkó
authors.forename: A
authors.initials: A
authors.lastname: Tóth
authors.forename: I
authors.initials: I
authors.lastname: Damjanova
authors.forename: G
authors.initials: G
authors.lastname: Mestyán
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D015780:Carbapenems / Q000494:pharmacology; D024901:Drug Resistance, Multiple, Bacterial; D016521:Electrophoresis, Gel, Pulsed-Field; D005798:Genes, Bacterial; D006785:Hospitals, University; D006801:Humans; D006814:Hungary / Q000453:epidemiology; D007710:Klebsiella Infections / Q000382:microbiology*; D007711:Klebsiella pneumoniae / Q000145:classification / Q000201:enzymology* / Q000302:isolation & purification*; D008826:Microbial Sensitivity Tests; D058889:Molecular Typing; D016133:Polymerase Chain Reaction; D001618:beta-Lactamases / Q000235:genetics* / Q000378:metabolism*
chemical_list: D000900:Anti-Bacterial Agents; D015780:Carbapenems; C473109:beta-lactamase CTX-M-15; D001618:beta-Lactamases; C482458:metallo-beta-lactamase VIM-4, Klebsiella pneumoniae
keywords: Carbapenemase; In238b; Klebsiella pneumoniae; VIM-4; sequence type 15