title: [Activation of immunoproteasome subunit genes transcription in mice monocytes infected with different strains of mycobacteria].
abstract: Immunoproteasomal processing of mycobacterial antigens is necessary to control the infection and to protect the organism from development of active form of tuberculosis. Here we investigate the activation of immunoproteasome subunit genes transcription in peritoneal monocytes of C57Bl/6 mice infected with vaccine M. bovis BCG and virulent strain M. tuberculosis H37Rv. The level of transcription of LMP2, LMP7, MECL1 subunits didn't increase for one and two days after a single infection. Two rounds of infection with BCG strain M. bovis led to enhancement of the only LMP7 subunit gene transcription. However after subsequent infection of monocytes with vaccine followed by virulent strain infection the dramatic rise of all immunoproteasomal subunit genes transcription was observed. Activation of transcription of the gene coding the PA28alpha subunit of regulatory complex PA28 was observed only after a single infection of monocytes with strain M. bovis BCG. Thus, vaccination with strain M. bovis BCG promotes effective activation of immunoproteasomal genes in case of subsequent contact with virulent strain M. tuberculosis H37Rv.
authors.forename: A V
authors.initials: AV
authors.lastname: Timofeev
authors.forename: Iu V
authors.initials: IuV
authors.lastname: Kuz'menko
authors.forename: I I
authors.initials: II
authors.lastname: Zharkov
authors.forename: E S
authors.initials: ES
authors.lastname: Starodubova
authors.forename: V L
authors.initials: VL
authors.lastname: Karpov
mesh_terms: D000818:Animals; D003546:Cysteine Endopeptidases / Q000096:biosynthesis / Q000276:immunology; D051379:Mice; D009000:Monocytes / Q000166:cytology / Q000276:immunology*; D009159:Mycobacteriaceae / Q000235:genetics / Q000276:immunology* / Q000472:pathogenicity; D046988:Proteasome Endopeptidase Complex / Q000096:biosynthesis / Q000235:genetics* / Q000276:immunology; D014158:Transcription, Genetic; D014376:Tuberculosis / Q000235:genetics* / Q000276:immunology / Q000382:microbiology
chemical_list: C071313:LMP-2 protein; D003546:Cysteine Endopeptidases; C077436:LMP7 protein; D046988:Proteasome Endopeptidase Complex; C490920:Psmb10 protein, mouse; C486213:Psme1 protein, mouse