title: Adenoviruses isolated from wild gorillas are closely related to human species C viruses.
abstract: We have isolated and cultured three distinct adenoviruses from wild gorillas. Phylogenetic analysis grouped the viruses with human adenovirus species C based on DNA polymerase, hexon, and E4ORF6 genes. The three wild gorilla adenoviruses clustered with the other species C captive gorilla adenoviruses, forming a branch separate from human and chimpanzee/bonobo adenoviruses. Animal sera to the three newly isolated viruses did not cross-neutralize, demonstrating serological distinctiveness. The human adenovirus 5 fiber knob blocked infection, suggesting use of the Coxsackie and Adenovirus Receptor. These viruses may provide viral vectors with properties distinct from chimpanzee adenovirus and human adenovirus vectors.
authors.affiliation: GenVec, Inc., 65W. Watkins Mill Rd, Gaithersburg, MD 20878, USA.
authors.forename: McVey
authors.initials: M
authors.lastname: Duncan
authors.forename: Michael R
authors.initials: MR
authors.lastname: Cranfield
authors.forename: Holly
authors.initials: H
authors.lastname: Torano
authors.forename: Hubert M
authors.initials: HM
authors.lastname: Kuete
authors.forename: Grace P
authors.initials: GP
authors.lastname: Lee
authors.forename: Andrew
authors.initials: A
authors.lastname: Glenn
authors.forename: Joseph T
authors.initials: JT
authors.lastname: Bruder
authors.forename: David
authors.initials: D
authors.lastname: Rangel
authors.forename: Douglas E
authors.initials: DE
authors.lastname: Brough
authors.forename: Jason G
authors.initials: JG
authors.lastname: Gall
mesh_terms: D000256:Adenoviridae / Q000145:classification* / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification; D000257:Adenoviridae Infections / Q000662:veterinary* / Q000821:virology; D000818:Animals; D057134:Antibodies, Neutralizing / Q000097:blood; D000914:Antibodies, Viral / Q000097:blood; D016000:Cluster Analysis; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D006071:Gorilla gorilla; D008969:Molecular Sequence Data; D009500:Neutralization Tests; D010802:Phylogeny; D018419:Primate Diseases / Q000821:virology*; D017422:Sequence Analysis, DNA; D012703:Serotyping
chemical_list: D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D004279:DNA, Viral
keywords: Adenovirus; Evolution; Gorilla; Non-human primate; Viral vectors