title: Microbiological evaluation of ultrasonic nebulization for disinfecting dental impressions.
abstract: Disinfecting dental impressions is necessary to decrease the risk of cross-contamination in dental offices. Ultrasonic nebulization has been mentioned as a microbicidal technique that can be used to disinfect contaminated dental impressions. This study compared the microbicidal effect of 2% glutaraldehyde and 0.2% peracetic acid for the disinfection of dental impressions made with vinyl polysiloxane, using 2 disinfection methods: immersion and ultrasonic nebulization. Bactericial efficacy was examined using Staphylococcus aureus and Bacillus atrophaeus as indicators. Thirty impressions were obtained and distributed randomly in 5 groups (n = 6). Group 1 was immersed in 2% glutaraldehyde immersion for 10 minutes, Group 2 was immersed in 0.2% peracetic acid for 10 minutes, Group 3 underwent ultrasonic nebulization for 10 minutes in 2% glutaraldehyde solution, Group 4 underwent ultrasonic nebulization for 10 minutes in 0.2% peracetic acid solution, and Group 5 was a control group that received no disinfectant. Both solutions experienced a 100% reduction in microorganisms following ultrasonic nebulization, as did peracetic acid following immersion; however, immersion in glutaraldehyde demonstrated lower values of reduction in B atrophaeus group, with a statistically significant difference compared with the other experimental groups.
authors.affiliation: Department of Prosthodontics, Dental School, Western Parana University, Cascavel, Brazil.
authors.forename: Marcio Jose
authors.initials: MJ
authors.lastname: Mendonca
authors.forename: Renata Santos
authors.initials: RS
authors.lastname: Rafael
authors.forename: Veridiana
authors.initials: V
authors.lastname: Camilotti
authors.forename: Rafael Andrade
authors.initials: RA
authors.lastname: Menolli
authors.forename: Eliseu Augusto
authors.initials: EA
authors.lastname: Sicoli
authors.forename: Nancielli
authors.initials: N
authors.lastname: Teixeira
authors.forename: Mario Alexandre Coelho
authors.initials: MA
authors.lastname: Sinhoreti
mesh_terms: D019551:Dental Disinfectants; D003760:Dental Impression Materials; D003761:Dental Impression Technique; D004202:Disinfectants; D004203:Disinfection; D005976:Glutaral; D013211:Staphylococcus aureus; D014465:Ultrasonics
chemical_list: D019551:Dental Disinfectants; D003760:Dental Impression Materials; D004202:Disinfectants; D005976:Glutaral
keywords: dental impression; disinfection; peracetic acid; ultrasonic nebulization