title: [The development and implementation of polymerase chain reaction to detect in real-time operation mode yersinia pestis in field material].
abstract: The article presents the results of development and practical implementation of system of polymerase chain reaction testing in real-time operation mode to detect agent of plague infield material. In laboratory conditions the system demonstrated good results and hence it was applied in conditions of field laboratory of epidemiologic team during planned epizootologic examination of Gorno-Altaisk hot spot of plague. The sampling consisted of more than 1400 objects. It was demonstrated that high sensitivity and specificity is immanent to proposed system. The adaptation of the system to the real time amplifier "Smart Cycler" (Cephid, USA) having some specific technical characteristics makes it possible to consider the proposed test-system as an effective sensitive and precise instrument for screening studies in the process of regular epizootologic examinations of hot spots of plague.
authors.forename: M V
authors.initials: MV
authors.lastname: Afanas'ev
authors.forename: E V
authors.initials: EV
authors.lastname: Chipanin
authors.forename: V E
authors.initials: VE
authors.lastname: Shestakov
authors.forename: A V
authors.initials: AV
authors.lastname: Denisov
authors.forename: L A
authors.initials: LA
authors.lastname: Fomina
authors.forename: A S
authors.initials: AS
authors.lastname: Ostiak
authors.forename: S V
authors.initials: SV
authors.lastname: Balakhonov
mesh_terms: D000818:Animals; D001483:Base Sequence; D004269:DNA, Bacterial / Q000235:genetics; D006801:Humans; D007313:Insecta / Q000382:microbiology; D010930:Plague / Q000175:diagnosis* / Q000382:microbiology* / Q000635:transmission; D016133:Polymerase Chain Reaction; D012377:Rodentia / Q000382:microbiology; D005423:Siphonaptera / Q000382:microbiology; D015010:Yersinia pestis / Q000302:isolation & purification* / Q000472:pathogenicity
chemical_list: D004269:DNA, Bacterial