title: Tumor necrosis factor-alfa and monocyte chemoattractant protein-1 gene polymorphisms in kidney transplant recipients.
abstract: Tumor necrosis factor-alfa (TNF-α) gene polymorphism is supposed to have a significant influence on the incidence of acute rejection in renal transplantation. The monocyte chemoattractant protein-1 (MCP-1) is another factor supposed to modulate graft rejection. We studied TNF-α and MCP-1 gene polymorphisms in 84 kidney allograft recipients with polymerase chain reaction and restriction fragment length polymorphism and their serum levels by enzyme-linked immunosorbent assay. The patients were classified into two groups based on their outcomes: Group I (n = 47) recipients with stable graft function as the control group and group II (n=37) recipients who experienced acute graft rejection episodes in the first 30 days post-transplantation. A significantly higher incidence of TNF 2 /TNF 2 genotype was observed among patients with acute graft rejection in comparison with the control group (40.5% and 19.2% respectively, P <0.05), while no statistically significant differences were observed in the TNF 1 /TNF 1 genotype between the groups (59.4% and 80.8%, respectively, P >0.05). A significant elevation of serum TNF-α levels was found in group II than group I and between TNF 2 genotype compared with that of TNF1 genotype within group II recipients. Distribution of MCP-1 genotypes in patients with and without acute rejection episodes was not significantly different (70.2% and 76.6% for MCP-1 A/A and 29.7% and 23.4% for MCP-1 G/G, respectively, P >0.05). The serum MCP-1 levels were not significantly different between the groups and between MCP-1 G/G genotype and MCP-1 A/A genotype in group II recipients. In conclusion, TNF-α gene polymorphism or its serum levels may identify patients at risk of acute rejection, where patients with TNF 2 /TNF 2 genotype or high serum TNF-α levels are more likely to have acute rejection episodes, while there was no relation between MCP-1 genotype or its serum levels and acute rejection.
authors.affiliation: Clinical Pathology Department, Assiut University, Assiut, Egypt.
authors.forename: Ebtesam M
authors.initials: EM
authors.lastname: El-Gezawy
authors.forename: Eman Nasr
authors.initials: EN
authors.lastname: Eldin
authors.forename: Wafaa S
authors.initials: WS
authors.lastname: Mohamed
authors.forename: Maged S
authors.initials: MS
authors.lastname: Mahmoud
authors.forename: Seham Ahmed
authors.initials: SA
authors.lastname: Saied
authors.forename: Hanan Hareth
authors.initials: HH
authors.lastname: Abd El-Latif
authors.forename: Maha Atwa
authors.initials: MA
authors.lastname: Ibrahim
mesh_terms: D000328:Adult; D018932:Chemokine CCL2 / Q000235:genetics*; D005260:Female; D006801:Humans; D016030:Kidney Transplantation; D008297:Male; D011110:Polymorphism, Genetic; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics*
chemical_list: D018932:Chemokine CCL2; D014409:Tumor Necrosis Factor-alpha