title: Secretoglobin expression in ovarian carcinoma: lipophilin B gene upregulation as an independent marker of better prognosis.
abstract: BACKGROUND
  The aim of the present study was to investigate within ovarian carcinoma and normal ovarian biopsies the gene expression of multiple secretoglobin family members relative to mammaglobin B, which we previously reported as a promising novel ovarian carcinoma prognostic marker.
  
  
  METHODS
  Using quantitative real-time Reverse Transcription PCR we tested 53 ovarian carcinoma and 30 normal ovaries for the expression of 8 genes belonging to the secretoglobin family: mammaglobin A, lipophilin A, lipophilin B, uteroglobin, HIN-1, UGRP-1, RYD5 and IIS. Next, we decided to expand the LipB gene expression analysis to a further 48 ovarian carcinoma samples, for a total of 101 tumor tissues of various histologies and to study its protein expression by immunohistochemistry in formalin-fixed paraffin-embedded tumors and normal ovaries. Finally, we correlated lipophilin B gene and protein expression to conventional patient clinico-pathological features and outcome.
  
  
  RESULTS
  We found significant mammaglobin A, lipophilin A, lipophilin B and RYD5 gene overexpression in ovarian carcinomas compared to normal ovaries. Lipophilin B mRNA showed a higher presence in tumors (75.4%) compared to normal ovaries (16.6%) and the most significant correlation with mammaglobin B mRNA (rs =0.77, p < 0.001). By immunohistochemical analysis, we showed higher lipophilin B expression in the cytoplasm of tumor cells compared to normal ovaries (p < 0.001). Moreover, lipophilin B gene overexpression was significantly associated with serous histology (serous vs clear cell p = 0.027; serous vs undifferentiated p = 0.007) and lower tumor grade (p = 0.02). Lower LipB mRNA levels (low versus high tertiles) were associated to a shorter progression-free (p = 0.03, HR = 2.2) and disease-free survival (p = 0.02, HR = 2.5) by univariate survival analysis and, importantly, they remain an independent prognostic marker for decreased disease-free (p = 0.001, HR = 3.9) and progression-free survival (p = 0.004, HR = 2.8) in multivariate Cox regression analysis.
  
  
  CONCLUSIONS
  The present study represents the first quantitative evaluation of secretoglobin gene expression in normal and neoplastic ovarian tissues. Our results demonstrate lipophilin B gene and protein upregulation in ovarian carcinoma compared to normal ovary. Moreover, lipophilin B gene overexpression correlates with a less aggressive tumor phenotype and represents a novel ovarian carcinoma prognostic factor.
authors.affiliation: Angelo Nocivelli Institute of Molecular Medicine, Division of Gynecologic Oncology, University of Brescia, Viale Europa 11, 25123 Brescia, Italy. bignottieliana@yahoo.it
authors.forename: Eliana
authors.initials: E
authors.lastname: Bignotti
authors.forename: Renata A
authors.initials: RA
authors.lastname: Tassi
authors.forename: Stefano
authors.initials: S
authors.lastname: Calza
authors.forename: Antonella
authors.initials: A
authors.lastname: Ravaggi
authors.forename: Elisa
authors.initials: E
authors.lastname: Rossi
authors.forename: Carla
authors.initials: C
authors.lastname: Donzelli
authors.forename: Paola
authors.initials: P
authors.lastname: Todeschini
authors.forename: Chiara
authors.initials: C
authors.lastname: Romani
authors.forename: Elisabetta
authors.initials: E
authors.lastname: Bandiera
authors.forename: Laura
authors.initials: L
authors.lastname: Zanotti
authors.forename: Mario
authors.initials: M
authors.lastname: Carnazza
authors.forename: Francesco
authors.initials: F
authors.lastname: Quadraro
authors.forename: Germana
authors.initials: G
authors.lastname: Tognon
authors.forename: Enrico
authors.initials: E
authors.lastname: Sartori
authors.forename: Sergio
authors.initials: S
authors.lastname: Pecorelli
authors.forename: Dana M
authors.initials: DM
authors.lastname: Roque
authors.forename: Alessandro D
authors.initials: AD
authors.lastname: Santin
mesh_terms: D000368:Aged; D014408:Biomarkers, Tumor / Q000378:metabolism; D018572:Disease-Free Survival; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D008875:Middle Aged; D010051:Ovarian Neoplasms / Q000175:diagnosis / Q000378:metabolism*; D010053:Ovary / Q000378:metabolism; D010641:Phenotype; D011379:Prognosis; D060888:Real-Time Polymerase Chain Reaction; D060765:Secretoglobins / Q000378:metabolism*; D016896:Treatment Outcome; D015854:Up-Regulation
chemical_list: D014408:Biomarkers, Tumor; C556464:SCGB1D2 protein, human; D060765:Secretoglobins