title: Effectiveness of heat treatment to protect introduced denitrifying bacteria from eukaryotic predatory microorganisms in a pilot-scale bioreactor.
abstract: Bioaugmentation of bioreactor systems with pre-cultured bacteria has proven difficult because inoculated bacteria are easily eliminated by predatory eukaryotic-microorganisms. Here, we demonstrated an intermediate thermal treatment was effective for protecting introduced denitrifying bacteria from eukaryotic predators and consequently allowed the inoculated bacteria to survive longer in a denitrification reactor.
authors.affiliation: Faculty of Engineering, Tohoku Gakuin University, Tagajo, Miyagi 985-8537, Japan.
authors.forename: Wakako
authors.initials: W
authors.lastname: Ikeda-Ohtsubo
authors.forename: Morio
authors.initials: M
authors.lastname: Miyahara
authors.forename: Takeshi
authors.initials: T
authors.lastname: Yamada
authors.forename: Akira
authors.initials: A
authors.lastname: Watanabe
authors.forename: Shinya
authors.initials: S
authors.lastname: Fushinobu
authors.forename: Takayoshi
authors.initials: T
authors.lastname: Wakagi
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Shoun
authors.forename: Keisuke
authors.initials: K
authors.lastname: Miyauchi
authors.forename: Ginro
authors.initials: G
authors.lastname: Endo
mesh_terms: D000818:Animals; D019149:Bioreactors / Q000382:microbiology*; D058440:Denitrification / Q000502:physiology*; D056890:Eukaryota / Q000235:genetics / Q000254:growth & development; D006358:Hot Temperature; D009566:Nitrates / Q000378:metabolism; D010865:Pilot Projects; D044223:Pseudomonas stutzeri / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism*; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D012722:Sewage / Q000382:microbiology; D013552:Swine; D062065:Wastewater / Q000382:microbiology*
chemical_list: D009566:Nitrates; D012337:RNA, Ribosomal, 18S; D012722:Sewage; D062065:Wastewater
keywords: 18S rRNA genes; Bioaugmentation; Denitrifying bacteria; Heat treatment; Predatory protists