title: Characterization of signal sequences determining the nuclear export of Newcastle disease virus matrix protein.
abstract: The Newcastle disease virus (NDV) matrix (M) protein has been demonstrated to be a nuclear-cytoplasmic trafficking protein. Previous studies have shown that the M protein localizes in the nucleus through a bipartite nuclear localization signal. Here, we report that the ability of the M protein to shuttle to the cytoplasm is mediated by three nuclear export signal sequences (NESs). Using leptomycin B (LMB), a specific inhibitor of CRM1, we found that the nuclear export of the three NESs was LMB insensitive and thus was CRM1 independent. In addition, inactivation of these NESs led to nuclear accumulation of the M protein. Our results highlight the significance of these NESs to the nuclear export of the NDV M protein.
authors.affiliation: Ministry of Education Key Lab for Avian Preventive Medicine, Yangzhou University, Yangzhou, Jiangsu, China.
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Duan
authors.forename: Qingqing
authors.initials: Q
authors.lastname: Song
authors.forename: Yuyang
authors.initials: Y
authors.lastname: Wang
authors.forename: Liang
authors.initials: L
authors.lastname: He
authors.forename: Jian
authors.initials: J
authors.lastname: Chen
authors.forename: Yanmei
authors.initials: Y
authors.lastname: Zhu
authors.forename: Shunlin
authors.initials: S
authors.lastname: Hu
authors.forename: Xiufan
authors.initials: X
authors.lastname: Liu
mesh_terms: D021581:Active Transport, Cell Nucleus; D000818:Animals; D016466:CHO Cells; D003412:Cricetulus; D009522:Newcastle disease virus / Q000235:genetics* / Q000502:physiology; D021382:Protein Sorting Signals; D014763:Viral Matrix Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: D021382:Protein Sorting Signals; D014763:Viral Matrix Proteins