title: Complete genome sequence of a novel dsRNA mycovirus isolated from the phytopathogenic fungus Verticillium dahliae Kleb.
abstract: A novel double-stranded RNA (dsRNA) mycovirus, designated Verticillium dahliae partitivirus 1 (VdPV1), was isolated from a strain of the fungus Verticillium dahliae. The VdPV1 genome has two dsRNA genome segments. The larger segment (1768 bp) has a single open reading frame (ORF) with a conserved RNA-dependent RNA polymerase (RdRP) domain. The smaller segment (1587 bp) contains a single ORF encoding a putative coat protein. Analysis of its genomic structure indicated that VdPV1 is a new member of the genus Partitivirus. We report the full-length sequence of this partitivirus that infects Verticillium dahliae, the causal agent of verticillium wilt of cotton.
authors.affiliation: State Key Laboratory of Cotton Biology, Cotton Research Institute of Chinese Academy of Agricultural Sciences, Anyang, 455000, Henan Province, China.
authors.forename: Zili
authors.initials: Z
authors.lastname: Feng
authors.forename: Heqin
authors.initials: H
authors.lastname: Zhu
authors.forename: Zhifang
authors.initials: Z
authors.lastname: Li
authors.forename: Yongqiang
authors.initials: Y
authors.lastname: Shi
authors.forename: Lihong
authors.initials: L
authors.lastname: Zhao
authors.forename: Lijiang
authors.initials: L
authors.lastname: Liu
authors.forename: Daohong
authors.initials: D
authors.lastname: Jiang
mesh_terms: D036022:Capsid Proteins / Q000235:genetics; D016000:Cluster Analysis; D016679:Genome, Viral; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010802:Phylogeny; D012328:RNA Viruses / Q000235:genetics* / Q000302:isolation & purification; D012330:RNA, Double-Stranded / Q000235:genetics; D012367:RNA, Viral / Q000235:genetics*; D012324:RNA-Dependent RNA Polymerase / Q000235:genetics; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D020172:Verticillium / Q000821:virology*
chemical_list: D036022:Capsid Proteins; D012330:RNA, Double-Stranded; D012367:RNA, Viral; D012324:RNA-Dependent RNA Polymerase