title: The effects of Batroxobin on the intimal hyperplasia of graft veins.
abstract: BACKGROUND
  To investigate the effects of Batroxobin (BX) on the intimal hyperplasia of graft veins.
  
  
  METHODS
  Twenty dogs were evenly divided into 2 groups. The femoral veins were grafted into the femoral artery by microsurgery, and the experimental group was treated with BX (0.1 BU/kg/48 hours). The serum level of endothelin-1 (ET-1) was detected 2 weeks after operation. Computer image analysis system was performed to calculate the cross-sectional area of neointima and media in the vein grafts 8 weeks after operation. Immunohistochemistry method was performed to identify proliferating cell nuclear antigen (PCNA) and c-Myc.
  
  
  RESULTS
  The experimental group had a lower level of serum ET-1 than the control group (P < .01), and both intimal hyperplasia and media thickness of graft veins were reduced by BX in comparison with the control group (P < .05). C-Myc expression was higher in the control group than in the experimental group (P < .01). The PCNA expression in the experimental group was significantly lower than the control group (P < .05).
  
  
  CONCLUSIONS
  These findings suggested that BX could inhibit intimal hyperplasia through suppressing cell proliferation activity.
authors.affiliation: Department of Emergent Surgery, Linyi's People's Hospital, Linyi City 276000, China. Electronic address: liuwg120@163.com.
authors.forename: Wenguang
authors.initials: W
authors.lastname: Liu
authors.forename: Jian
authors.initials: J
authors.lastname: Wang
authors.forename: Cheng
authors.initials: C
authors.lastname: Chen
authors.forename: Fan
authors.initials: F
authors.lastname: Zhou
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhou
authors.forename: Fangjie
authors.initials: F
authors.lastname: Zhang
mesh_terms: D000818:Animals; D012103:Batroxobin / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects; D004285:Dogs; D019332:Endothelin-1 / Q000097:blood; D005263:Femoral Artery / Q000601:surgery; D005268:Femoral Vein / Q000637:transplantation*; D005343:Fibrinolytic Agents / Q000494:pharmacology*; D006965:Hyperplasia; D007150:Immunohistochemistry; D008866:Microsurgery; D023421:Models, Animal; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D016271:Proto-Oncogene Proteins c-myc / Q000378:metabolism; D011897:Random Allocation; D014182:Transplantation, Autologous; D017539:Tunica Intima / Q000187:drug effects* / Q000473:pathology; D017540:Tunica Media / Q000187:drug effects* / Q000473:pathology
chemical_list: D019332:Endothelin-1; D005343:Fibrinolytic Agents; D018809:Proliferating Cell Nuclear Antigen; D016271:Proto-Oncogene Proteins c-myc; D012103:Batroxobin
keywords: Autologous veins graft; Batroxobin; Intimal hyperplasia