title: Human DNA extraction from empty puparia.
abstract: Empty puparia, as well as larvae at different developmental stages, are potentially useful in the identification of a victim where a corpse has been removed from the scene of a forensic investigation. To evaluate the relevance and the reliability of this substrate to be used as forensic evidence, the authors report for the first time the extraction and typing of human DNA from empty puparia using STR analysis, in two actual cases where the bodies of the victims were still present thereby enabling validation of the typing.
authors.affiliation: Institute of Legal Medicine, Catholic University, Medical School, Rome, Italy.
authors.forename: Daniela
authors.initials: D
authors.lastname: Marchetti
authors.forename: Elisa
authors.initials: E
authors.lastname: Arena
authors.forename: Ilaria
authors.initials: I
authors.lastname: Boschi
authors.forename: Stefano
authors.initials: S
authors.lastname: Vanin
mesh_terms: D000818:Animals; D004247:DNA / Q000302:isolation & purification*; D016172:DNA Fingerprinting / Q000379:methods*; D005247:Feeding Behavior; D005260:Female; D006801:Humans; D008297:Male; D018895:Microsatellite Repeats; D008875:Middle Aged; D011180:Postmortem Changes; D011679:Pupa; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D004247:DNA