title: Deciphering the response of Mycobacterium smegmatis to nitrogen stress using bipartite active modules.
abstract: BACKGROUND
  The ability to adapt to environments with fluctuating nutrient availability is vital for bacterial survival. Although essential for growth, few nitrogen metabolism genes have been identified or fully characterised in mycobacteria and nitrogen stress survival mechanisms are unknown.
  
  
  RESULTS
  A global transcriptional analysis of the mycobacterial response to nitrogen stress, showed a significant change in the differential expression of 16% of the Mycobacterium smegmatis genome. Gene expression changes were mapped onto the metabolic network using Active Modules for Bipartite Networks (AMBIENT) to identify metabolic pathways showing coordinated transcriptional responses to the stress. AMBIENT revealed several key features of the metabolic response not identified by KEGG enrichment alone. Down regulated reactions were associated with the general reduction in cellular metabolism as a consequence of reduced growth rate. Up-regulated modules highlighted metabolic changes in nitrogen assimilation and scavenging, as well as reactions involved in hydrogen peroxide metabolism, carbon scavenging and energy generation.
  
  
  CONCLUSIONS
  Application of an Active Modules algorithm to transcriptomic data identified key metabolic reactions and pathways altered in response to nitrogen stress, which are central to survival under nitrogen limiting environments.
authors.affiliation: Department of Medicine, MRC Centre for Molecular Bacteriology and Infection, South Kensington, London SW7 2AZ, UK.
authors.forename: Kerstin J
authors.initials: KJ
authors.lastname: Williams
authors.forename: William A
authors.initials: WA
authors.lastname: Bryant
authors.forename: Victoria A
authors.initials: VA
authors.lastname: Jenkins
authors.forename: Geraint R
authors.initials: GR
authors.lastname: Barton
authors.forename: Adam A
authors.initials: AA
authors.lastname: Witney
authors.forename: John W
authors.initials: JW
authors.lastname: Pinney
authors.forename: Brian D
authors.initials: BD
authors.lastname: Robertson
mesh_terms: D000465:Algorithms; D020869:Gene Expression Profiling; D016680:Genome, Bacterial / Q000235:genetics; D023281:Genomics / Q000379:methods*; D006861:Hydrogen Peroxide / Q000378:metabolism; D020102:Mycobacterium smegmatis / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism / Q000502:physiology*; D009584:Nitrogen / Q000494:pharmacology*; D013312:Stress, Physiological / Q000187:drug effects* / Q000235:genetics*
chemical_list: D006861:Hydrogen Peroxide; D009584:Nitrogen