title: Molecular view on protein sorting into liquid-ordered membrane domains mediated by gangliosides and lipid anchors.
abstract: We present results from coarse grain molecular dynamics simulations of mixed model membranes consisting of saturated and unsaturated lipids together with cholesterol, in which lipid-anchored membrane proteins are embedded. The membrane proteins studied are the peripherally bound H-Ras, N-Ras, and Hedgehog, and the transmembrane peptides WALP and LAT. We provide a molecular view on how the presence and nature of these lipid anchors affects partitioning of the proteins between liquid-ordered and liquid-disordered domains. In addition, we probed the role of the ganglioside lipid GM1 on the protein sorting, showing formation of GM1-protein nano-domains that act as shuttles between the differently ordered membrane regions.
authors.forename: Djurre H
authors.initials: DH
authors.lastname: de Jong
authors.forename: Cesar A
authors.initials: CA
authors.lastname: Lopez
authors.forename: Siewert J
authors.initials: SJ
authors.lastname: Marrink
mesh_terms: D000595:Amino Acid Sequence; D005677:G(M1) Ganglioside / Q000737:chemistry / Q000378:metabolism; D005732:Gangliosides / Q000737:chemistry / Q000378:metabolism*; D053823:Hedgehog Proteins / Q000737:chemistry / Q000378:metabolism; D058635:Lipid-Linked Proteins / Q000737:chemistry / Q000378:metabolism*; D021962:Membrane Microdomains / Q000737:chemistry* / Q000378:metabolism*; D056004:Molecular Dynamics Simulation; D008969:Molecular Sequence Data; D017434:Protein Structure, Tertiary; D021381:Protein Transport; D018631:ras Proteins / Q000737:chemistry / Q000378:metabolism*
chemical_list: D005732:Gangliosides; D053823:Hedgehog Proteins; D058635:Lipid-Linked Proteins; D005677:G(M1) Ganglioside; D018631:ras Proteins