title: An innovative glucose biosensor using antibiofouling Au-F127 nanospheres.
abstract: Quantification of the blood glucose concentration in the whole blood was not easy to achieve because the detection process was affected by many factors, such as glucose metabolism and biofouling. In this paper, we established an amperometric glucose biosensor applied in whole blood directly, which was based on the direct electron transfer of glucose oxidase (GOx) entrapped onto the Au-F127 nanospheres. Here, the Au-F127 nanospheres could provide a blood compatible surface with antifouling property for determination of glucose in whole blood. The cyclic voltammetric results indicated that GOx immobilized on the Au-F127 nanospheres exhibited direct electron transfer reaction, and the cyclic voltammogram (CV) displayed a pair of well-defined and nearly symmetric redox peaks with a formal potential of 93 mV. The biosensor had good electrocatalytic activity toward glucose with a low detection limit 3.15 pM. The glucose biosensor did not respond to ascorbic acid (AA) and uric acid (UA) at their high concentration encountered in blood. In this method, the biosensor was used for quantification of the concentration of glucose in whole blood samples. The data obtained from the biosensor showed good agreement with those from a biochemical analyzer in hospital.
authors.affiliation: Jiangsu Key Laboratory of Biofunctional Materials, College of Chemistry and Materials Science, Nanjing Normal University, Nanjing 210023, PR China.
authors.forename: Chong
authors.initials: C
authors.lastname: Sun
authors.forename: Xiaobo
authors.initials: X
authors.lastname: Wangi
authors.forename: Min
authors.initials: M
authors.lastname: Zhou
authors.forename: Yalong
authors.initials: Y
authors.lastname: Ni
authors.forename: Chun
authors.initials: C
authors.lastname: Mao
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Huang
authors.forename: Jian
authors.initials: J
authors.lastname: Shenl
mesh_terms: D057219:Biofouling / Q000517:prevention & control*; D015374:Biosensing Techniques; D001773:Blood Cells / Q000187:drug effects / Q000502:physiology; D001780:Blood Coagulation Tests; D001786:Blood Glucose / Q000032:analysis*; D002384:Catalysis; D002448:Cell Adhesion / Q000187:drug effects; D005949:Glucose Oxidase / Q000737:chemistry / Q000378:metabolism; D006046:Gold / Q000737:chemistry*; D006801:Humans; D062069:Inventions; D053768:Metal Nanoparticles / Q000737:chemistry*; D054118:Nanospheres / Q000737:chemistry*; D011095:Polyethylenes / Q000737:chemistry* / Q000494:pharmacology; D011126:Polypropylenes / Q000737:chemistry* / Q000494:pharmacology; D012969:Sodium Fluoride / Q000494:pharmacology
chemical_list: D001786:Blood Glucose; D011095:Polyethylenes; D011126:Polypropylenes; D006046:Gold; D012969:Sodium Fluoride; C078661:UCON 50-HB-5100; D005949:Glucose Oxidase