title: Activity of lipase and chitinase immobilized on superparamagnetic particles in a rotational magnetic field.
abstract: We immobilize hydrolases such as lipase and chitinase on superparamagnetic particles, which are subjected to a rotational magnetic field, and measure the activities of the enzymes. We find that the activities of lipase and chitinase increase in the rotational magnetic field compared to those in the absence of a magnetic field and reach maximum at certain frequencies. The present methodology may well be utilized for the design and development of efficient micro reactors and micro total analysis systems (μ-TASs).
authors.affiliation: Bio-Nano Electronics Research Centre, Toyo University, Saitama, Japan.
authors.forename: Toru
authors.initials: T
authors.lastname: Mizuki
authors.forename: Miyuki
authors.initials: M
authors.lastname: Sawai
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Nagaoka
authors.forename: Hisao
authors.initials: H
authors.lastname: Morimoto
authors.forename: Toru
authors.initials: T
authors.lastname: Maekawa
mesh_terms: D002176:Candida albicans / Q000201:enzymology; D002688:Chitinases / Q000737:chemistry*; D004800:Enzymes, Immobilized / Q000737:chemistry*; D005656:Fungal Proteins / Q000737:chemistry*; D008049:Lipase / Q000737:chemistry*; D060526:Magnetic Fields; D053758:Nanoparticles / Q000737:chemistry*; D014242:Trichoderma / Q000201:enzymology
chemical_list: D004800:Enzymes, Immobilized; D005656:Fungal Proteins; D008049:Lipase; D002688:Chitinases