title: [Impact of oral vitamin D supplementation in early life on diabetic mice induced by streptozotocin].
abstract: OBJECTIVE
  To evaluate the effect of oral vitamin D supplementation in early life on blood glucose, insulin content, diabetes incidence, and histomorphology in pancreatic islet induced by streptozotocin.
  
  
  METHODS
  Three-week-old C57BL/6 mice were given either control diet (American Institute of Nutrition [AIN]-93G), or three different dose of vitamin D-supplemented diet. Nine weeks after dietary intervention, C57BL/6 mice were treated with streptozotocin i.p. for 5 consecutive days. After injection of STZ, The fasting blood glucose and diabetes incidence was tested once a week. The insulin content, histomorphology in pancreatic islets was conducted at the end of experiments.
  
  
  RESULTS
  (1) Vitamin D supplementation in early life can decrease the fasting blood glucose values induced by STZ, and the decreases effect of high dose vitamin D-supplemented group is the most significant (P<0.01). (2) Vitamin D supplementation in early life can prevent diabetes incidence, and the decreases effect of high dose vitamin D-supplemented group is the most significant, fully suppress the onset of diabetes about four weeks later after injected by STZ (P<0.01). (3) As compared to the control group, insulin content in medium and high dose vitamin D-supplemented groups were significantly up-regulated after injection of STZ (P<0.05), and the effect of high dose vitamin D-supplemented group is the most significant. (4) The damage of pancreatic islets induced by STZ was clearly restored in medium and high dose vitamin D-supplemented groups, and effect of high dose vitamin D-supplemented group is the most significant.
  
  
  CONCLUSIONS
  Oral vitamin D supplementation in early life can decrease the fasting blood glucose values, prevent diabetes incidence, up-regulate insulin content, restored the damage of pancreatic islets induced by STZ, and effects of high dose vitamin D-supplementde group are all the most significant.
authors.affiliation: Department of Nutrition & Food Hygiene, School of Public Health, Anhui Medical University, Hefei 230032, China. guoxiu945@163.com
authors.forename: Guoxiu
authors.initials: G
authors.lastname: Wang
authors.forename: Chuanlai
authors.initials: C
authors.lastname: Hu
authors.forename: Chunqiu
authors.initials: C
authors.lastname: Hu
authors.forename: Liang
authors.initials: L
authors.lastname: Ruan
authors.forename: Qingli
authors.initials: Q
authors.lastname: Bo
authors.forename: Li
authors.initials: L
authors.lastname: Li
mesh_terms: D000284:Administration, Oral; D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000517:prevention & control*; D004305:Dose-Response Relationship, Drug; D005260:Female; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D013997:Time Factors; D014807:Vitamin D / Q000627:therapeutic use*
chemical_list: D014807:Vitamin D