title: Canine kobuviruses in diarrhoeic dogs in Italy.
abstract: Canine kobuviruses (CaKVs) are newly recognized picornaviruses recently detected in dogs in the US. By molecular analysis of the whole genome, CaKV that appeared genetically closest to the murine kobuvirus (MuKV) and to the human Aichi virus (AiV), may be classified in the Kobuvirus genus as new genotype (CaKV type 1) within the species Aichivirus A. To date, there are no information on the epidemiology of these novel viruses in other continents. In this study, by screening a collection of 256 dog fecal samples either from diarrhoeic or asymptomatic animals, CaKV was identified in six specimens with an overall prevalence of 2.34% (6/256). All the positive dogs presented diarrhea and were found to be infected by CaKV alone or in mixed infections with canine coronavirus (CCoV) and/or canine parvovirus type 2 (CPV-2). By molecular analysis of the partial 3D gene, all the strains detected displayed a close relatedness with the CaKVs recently identified in the US. This study provides evidence that CaKVs circulate in diarrhoeic dogs in Italy and are not geographically restricted to the North American continent, where they were first signaled.
authors.affiliation: Department of Scienze Biomediche Comparate, University of Teramo, Teramo, Italy. bdimartino@unite.it
authors.forename: Barbara
authors.initials: B
authors.lastname: Di Martino
authors.forename: Elisabetta
authors.initials: E
authors.lastname: Di Felice
authors.forename: Chiara
authors.initials: C
authors.lastname: Ceci
authors.forename: Federica
authors.initials: F
authors.lastname: Di Profio
authors.forename: Fulvio
authors.initials: F
authors.lastname: Marsilio
mesh_terms: D000818:Animals; D003967:Diarrhea / Q000662:veterinary* / Q000821:virology; D004283:Dog Diseases / Q000453:epidemiology / Q000821:virology*; D004285:Dogs; D005243:Feces / Q000821:virology; D007558:Italy / Q000453:epidemiology; D052699:Kobuvirus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D010802:Phylogeny; D010850:Picornaviridae Infections / Q000662:veterinary* / Q000821:virology
keywords: Canine kobuviruses; Dog; Picornaviruses