title: Characterization of the complete mitochondrial genome of Diaphania pyloalis (Lepidoptera: Pyralididae).
abstract: The complete mitochondrial genome (mitogenome) of Diaphania pyloalis (Lepidoptera: Pyralididae) was determined to be 15,298 bp and has the typical gene organization of mitogenomes from lepidopteran insects. It consists of 13 protein-coding genes (PCGs), two rRNA genes, 22 tRNA genes and an A+T-rich region. The A+T content of this mitogenome is 80.83% and the AT skew is slightly positive. All PCGs are initiated by ATN codons, except for cytochrome c oxidase subunit 1 (cox1) gene which is initiated by CGA. Only the cox2 gene has an incomplete stop codon consisting of just a T. All the tRNA genes display a typical clover-leaf structure of mitochondrial tRNA. The A+T-rich region of the mitogenome is 332 bp in length, including several common features found in lepidopteran mitogenomes. Phylogenetic analysis showed that the D. pyloalis is close to Pyralididae.
authors.affiliation: College of Life Science, Anhui Agricultural University, 130 Changjiang West Road, 230036, PR China.
authors.forename: Bao-Jian
authors.initials: BJ
authors.lastname: Zhu
authors.forename: Qiu-Ning
authors.initials: QN
authors.lastname: Liu
authors.forename: Li-Shang
authors.initials: LS
authors.lastname: Dai
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.forename: Yu
authors.initials: Y
authors.lastname: Sun
authors.forename: Kun-Zhang
authors.initials: KZ
authors.lastname: Lin
authors.forename: Guo-Qing
authors.initials: GQ
authors.lastname: Wei
authors.forename: Chao-Liang
authors.initials: CL
authors.lastname: Liu
mesh_terms: D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D017344:Genes, Insect; D054629:Genome, Mitochondrial; D055029:Inverted Repeat Sequences; D058977:Molecular Sequence Annotation; D008969:Molecular Sequence Data; D009036:Moths / Q000235:genetics*; D009690:Nucleic Acid Conformation; D010802:Phylogeny; D012343:RNA, Transfer / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D012343:RNA, Transfer
keywords: ATPase; Diaphania pyloalis; Lepidoptera; Mitochondrial genome; NADH; PCR; Phylogeny; adenosine triphosphatase; nicotinamide adenine dinucleotide hydrogen; polymerase chain reaction