title: [Experimental study on vascular bundle implantation combined with cellular transplantation in treating rabbit femoral head necrosis].
abstract: OBJECTIVE
  To discuss the feasibility of vascular bundle implantation combined with allogeneic bone marrow stromal cells (BMSCs) transplantation in treating rabbit femoral head osteonecrosis and bone defect, in order to explore a new method for the treatment of femoral head necrosis.
  
  
  METHODS
  Thirty-six New Zealand rabbits were randomly divided into three groups,with 12 rabbits in each group. Bilateral femoral heads of the rabbits were studied in the experiment. The models were made by liquid nitrogen frozen, and the femoral heads were drilled to cause bone defect. Group A was the control group,group B was stem cells transplantaion group of allograft marrow stromal,and group C was stem cells transplantation group of allograft marrow stromal combined with vascular bundle implantation. Three rabbits of each group were sacrificed respectively at 2, 4, 8, 12 weeks after operation. All specimens of the femoral heads were sliced for HE staining. Furthermore ,vascular density and the percentage of new bone trabecula of femoral head coronary section in defect area were measured and analyzed statistically.
  
  
  RESULTS
  In group C,new bone trabecula and original micrangium formed at the 2nd week after operation; new bone trabecula was lamellar and interlaced with abundant micrangium at the 8th week;at the 12th week,the broadened,coarsened bone trabecula lined up regularly,and the mature bone trabecula and new marrow were visible. At the 2nd week after operation,there was no statistical significance in the percentage of new bone trabecula of femoral head coronary section in defect area between group B and C. While at 4, 8, 12 week after operation, vascular density and the percentage of new bone trabecula of femoral head coronary section in defect area of group C was higher than that of group B.
  
  
  CONCLUSIONS
  Allogeneic bone marrow stromal cells cultured in vivo can form new bone trabecula, and can be applied to allotransplant. Vascular bundle implanted into the bone defect area of femoral head necrosis could improve blood supply, and promote the formation of bone trabecula.
authors.affiliation: chenshuangtao@126.com
authors.forename: Shuang-Tao
authors.initials: ST
authors.lastname: Chen
authors.forename: Wei-Ping
authors.initials: WP
authors.lastname: Zhang
authors.forename: Chang-An
authors.initials: CA
authors.lastname: Liu
authors.forename: Jun-Jiang
authors.initials: JJ
authors.lastname: Wang
authors.forename: Heng-Yi
authors.initials: HY
authors.lastname: Song
authors.forename: Zhi-wen
authors.initials: ZW
authors.lastname: Chai
mesh_terms: D000818:Animals; D001808:Blood Vessels / Q000637:transplantation*; D003131:Combined Modality Therapy; D005260:Female; D005271:Femur Head Necrosis / Q000473:pathology / Q000601:surgery*; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells; D011817:Rabbits; D014184:Transplantation, Homologous