title: Injectable and biodegradable sugar beet pectin/gelatin hydrogels for biomedical applications.
abstract: Injectable hydrogels have advantages over pre-formed hydrogels in biomedical applications. In our previous study, we showed usefulness of sugar beet pectin (SBP) as an injectable gel material. However, the in vivo biodegradability of the gels was low because animals lack suitable hydrolytic enzymes of SBP. In this study we developed SBP-based injectable gels with higher in vivo biodegradability than the previous SBP gels by incorporating biodegradable gelatin into the latter. An aqueous solution with dissolved SBP and gelatin rapidly (< 1 min) formed gels through a horseradish peroxidase-catalyzed oxidative coupling reaction between feruloyl moieties on the SBP molecules and phenolic moieties on the gelatin molecules. Gelation time and mechanical properties of the gels were tunable by adjusting the polymer concentrations. The gels containing doxorubicin, an anti-cancer drug, successfully suppressed the growth of a solid tumor created by subcutaneous injection of mouse melanoma B16F1 cells into nude mice. These results indicate that injectable and biodegradable SBP/gelatin gels are useful in biomedical applications.
authors.affiliation: Department of Chemical Engineering, Graduate School of Science and Engineering, Kagoshima University, 1-21-40 Korimoto, Kagoshima, 890-0065, Japan. takei@cen.kagoshima-u.ac.jp
authors.forename: Takayuki
authors.initials: T
authors.lastname: Takei
authors.forename: Kotaro
authors.initials: K
authors.lastname: Sugihara
authors.forename: Masahiro
authors.initials: M
authors.lastname: Yoshida
authors.forename: Koei
authors.initials: K
authors.lastname: Kawakami
mesh_terms: D020341:Absorbable Implants; D000818:Animals; D027461:Beta vulgaris / Q000737:chemistry*; D020811:Biomedical Technology / Q000379:methods*; D045744:Cell Line, Tumor; D004317:Doxorubicin / Q000008:administration & dosage; D004337:Drug Carriers / Q000008:administration & dosage / Q000138:chemical synthesis* / Q000737:chemistry / Q000493:pharmacokinetics; D005780:Gelatin / Q000008:administration & dosage* / Q000737:chemistry / Q000493:pharmacokinetics; D020100:Hydrogels / Q000008:administration & dosage* / Q000737:chemistry / Q000493:pharmacokinetics; D007267:Injections; D008297:Male; D008546:Melanoma, Experimental / Q000188:drug therapy / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D010368:Pectins / Q000008:administration & dosage* / Q000737:chemistry / Q000493:pharmacokinetics; D012878:Skin Neoplasms / Q000188:drug therapy / Q000473:pathology
chemical_list: D004337:Drug Carriers; D020100:Hydrogels; D004317:Doxorubicin; D010368:Pectins; D005780:Gelatin