title: Quantification and visualization of neutrophil extracellular traps (NETs) from murine bone marrow-derived neutrophils.
abstract: Neutrophils are one of the first cells to respond to an inflammatory stimulus, and are equipped with an assortment of antimicrobial and proteolytic enzymes to disarm and degrade bacterial pathogens. A novel mechanism of bacterial trapping, termed neutrophil extracellular traps (NETs), was recently described whereby neutrophils were shown to cast out web-like structures of chromatin, capturing and immobilizing invading pathogens. Herein we describe protocols to isolate murine bone marrow-derived neutrophils, and spectrophotometrically quantify, immunolabel, and visualize NET structures in vitro.
authors.affiliation: Faculty of Medicine, University of Toronto, Toronto, ON, Canada.
authors.forename: Linda
authors.initials: L
authors.lastname: Vong
authors.forename: Philip M
authors.initials: PM
authors.lastname: Sherman
authors.forename: Michael
authors.initials: M
authors.lastname: Glogauer
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology* / Q000276:immunology; D018929:Cell Culture Techniques / Q000379:methods*; D002843:Chromatin / Q000276:immunology / Q000378:metabolism; D007249:Inflammation / Q000378:metabolism / Q000473:pathology; D051379:Mice; D009504:Neutrophils / Q000166:cytology* / Q000276:immunology
chemical_list: D002843:Chromatin