title: Screening of Bunium bulbocastanum for antibacterial, antifungal, phytotoxic and haemagglutination activities.
abstract: The current study was aimed at screening the Bunium bulbocastanum for its antibacterial, antifungal, phytotoxic and haemagglutination activities.The crude methanolic extract and n-hexane fraction showed significant (89%) and good activity (61%) against Staphylococcus aureus while the CHCl3fraction was moderately active against S.aureus (53%). Moderate activitywas shown by the EtOAc fraction against B. subtilis (44%). This fraction was inactive against P.aerogenosa and S.aureus. The aqueous fraction showed significant activity against B. subtilis (85%), moderate against S.aureus(34 %) and E. coli (33%)and low activity against P.aerogenosa(29%). Our results for antifungal assay indicated that all the test samples were inactive against all the test fungi. The phytotoxic activity of the plant at 1000 and 100 μg/ml was: crude methanolic extract (53.33 and 46.66%), n-hexane (46.66 and 26.66%), CHCl3 (20 and 6.66%), EtOAc (46.66 and 26.66%) and aqueous (40 and 33.33%). All the test samples (crude methanolic extract and fractions) of B. bulbocastanum were unable to agglutinate RBCs of the human blood indicating that this species lack phytolectins.
authors.affiliation: Centre of Biotechnology and Microbiology, University of Peshawar, KhyberPukhtoonKhwa, Pakistan. ibrarkhan1984@upesh.edu.pk
authors.forename: Ibrar
authors.initials: I
authors.lastname: Khan
authors.forename: Haroon
authors.initials: H
authors.lastname: Ahmad
authors.forename: Nasir
authors.initials: N
authors.lastname: Ali
authors.forename: Bashir
authors.initials: B
authors.lastname: Ahmad
authors.forename: Hamid
authors.initials: H
authors.lastname: Tanoli
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D000935:Antifungal Agents / Q000494:pharmacology*; D019661:Apiaceae; D006384:Hemagglutination / Q000187:drug effects*; D010936:Plant Extracts / Q000494:pharmacology*; D010944:Plants / Q000187:drug effects
chemical_list: D000900:Anti-Bacterial Agents; D000935:Antifungal Agents; D010936:Plant Extracts