title: Epilobium angustifolium extract demonstrates multiple effects on dermal fibroblasts in vitro and skin photo-protection in vivo.
abstract: Stress-induced fibroblast senescence is thought to contribute to skin aging. Ultraviolet light (UV) radiation is the most potent environmental risk factor in these processes. An Epilobium angustifolium (EA) extract was evaluated for its capacity to reverse the senescent response of normal human dermal fibroblasts (NHDF) in vitro and to exhibit skin photo-protection in vivo. The HPLC-UV-MS analysis of the EA preparation identified three major polyphenol groups: tannins (oenothein B), phenolic acids (gallic and chlorogenic acids) and flavonoids. EA extract increased the cell viability of senescent NHDF induced by serum deprivation. It diminished connective tissue growth factor and fibronectin gene expressions in senescent NHDF. Down-regulation of the UV-induced release of both matrix metalloproteinase-1 and -3 and the tissue inhibitor of matrix metalloproteinases-1 and -2, and also down-regulation of the gene expression of hyaluronidase 2 were observed in repeatedly UV-irradiated NHDF after EA extract treatment. Interestingly, EA extract diminished the down-regulation of sirtuin 1 dampened by UV-irradiation. The application of EA extract using a sub-irritating dose protected skin against UV-induced erythema formation in vivo. In summary, EA extract diminished stress-induced effects on NHDF, particularly on connective tissue growth factor, fibronectin and matrix metalloproteinases. These results collectively suggest that EA extract may possess anti-aging properties and that the EA polyphenols might account for these benefits.
authors.affiliation: Contipro Biotech s.r.o., Dolni Dobrouc, Czech Republic. ema.ruszova@fnhk.cz.
authors.forename: Ema
authors.initials: E
authors.lastname: Ruszová
authors.forename: José
authors.initials: J
authors.lastname: Cheel
authors.forename: Stanislav
authors.initials: S
authors.lastname: Pávek
authors.forename: Martina
authors.initials: M
authors.lastname: Moravcová
authors.forename: Martina
authors.initials: M
authors.lastname: Hermannová
authors.forename: Ilona
authors.initials: I
authors.lastname: Matějková
authors.forename: Jiřina
authors.initials: J
authors.lastname: Spilková
authors.forename: Vladimír
authors.initials: V
authors.lastname: Velebný
authors.forename: Lukáš
authors.initials: L
authors.lastname: Kubala
mesh_terms: D000293:Adolescent; D000328:Adult; D015815:Cell Adhesion Molecules / Q000235:genetics; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D016922:Cellular Senescence / Q000187:drug effects / Q000528:radiation effects; D002648:Child; D055513:Connective Tissue Growth Factor / Q000235:genetics; D015536:Down-Regulation / Q000187:drug effects / Q000528:radiation effects; D031666:Epilobium / Q000737:chemistry*; D004890:Erythema / Q000188:drug therapy / Q000209:etiology; D005109:Extracellular Matrix / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects*; D005353:Fibronectins / Q000235:genetics; D058851:GPI-Linked Proteins / Q000235:genetics; D005786:Gene Expression Regulation / Q000187:drug effects / Q000528:radiation effects; D006801:Humans; D006821:Hyaluronoglucosaminidase / Q000235:genetics; D010641:Phenotype; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D011837:Radiation-Protective Agents / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D056564:Sirtuin 1 / Q000235:genetics; D012867:Skin / Q000166:cytology* / Q000187:drug effects / Q000528:radiation effects; D015595:Skin Aging / Q000187:drug effects / Q000528:radiation effects; D014466:Ultraviolet Rays / Q000009:adverse effects; D055815:Young Adult
chemical_list: C526317:CCN2 protein, human; D015815:Cell Adhesion Molecules; D005353:Fibronectins; D058851:GPI-Linked Proteins; D010936:Plant Extracts; D011837:Radiation-Protective Agents; D055513:Connective Tissue Growth Factor; C473096:Hyal2 protein, human; D006821:Hyaluronoglucosaminidase; C447939:SIRT1 protein, human; D056564:Sirtuin 1