title: Bone marrow-derived dendritic cells.
abstract: While much is understood about dendritic cells and their role in the immune system, the study of these cells is critical to gain a more complete understanding of their function. Dendritic cell isolation from mouse body tissues can be difficult and the number of cells isolated small. This protocol describes the growth of large number of dendritic cells from the culture of mouse bone marrow cells. The dendritic cells grown in culture facilitate experiments that may require large number of dendritic cells without great expense or use of large number of mice.
authors.affiliation: RTI International, Research Triangle Park, Durham, NC, USA.
authors.forename: Kelly
authors.initials: K
authors.lastname: Roney
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology* / Q000276:immunology; D018929:Cell Culture Techniques / Q000379:methods*; D002469:Cell Separation; D003713:Dendritic Cells / Q000166:cytology* / Q000276:immunology; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000276:immunology; D051379:Mice
chemical_list: D016178:Granulocyte-Macrophage Colony-Stimulating Factor