title: In vitro study of the cytotoxicity and antiproliferative effects of surfactants produced by Sphingobacterium detergens.
abstract: The application of biosurfactants in the biomedical field is growing due to their antimicrobial activity, low cytotoxicity and ability to induce apoptosis in cancer cells. In the light of this therapeutic potential, as well as possible applications in cosmetics or as drug vehicles in pharmaceutical products, a new biosurfactant produced by Sphingobacterium detergens was investigated for its haemolytic activity and cytotoxic and antiproliferative effects in different cell lines. Fraction A showed 100% haemolysis in rabbit erythrocytes, but in Fraction B the rate was only 83%. When comparing cytotoxicity values (IC50) of the two fractions in model fibroblast and keratinocyte cell cultures, Fraction B was less cytotoxic, showing lower values than the reference compound SDS, indicating low skin irritability. Finally, in non-differentiated intestinal Caco-2 cultures, Fractions A and B reduced cell proliferation and induced apoptosis by 44% and 75%, respectively. According to these results, biosurfactants produced by S. detergens have potential application in cosmetic and pharmaceutical formulations.
authors.affiliation: Laboratori de Microbiologia, Facultat de Farmàcia, Universitat de Barcelona. Av. Joan XIII s/n, 08028 Barcelona, Spain.
authors.forename: César
authors.initials: C
authors.lastname: Burgos-Díaz
authors.forename: Raquel
authors.initials: R
authors.lastname: Martín-Venegas
authors.forename: Verónica
authors.initials: V
authors.lastname: Martínez
authors.forename: Carolina Emilia
authors.initials: CE
authors.lastname: Storniolo
authors.forename: José Antonio
authors.initials: JA
authors.lastname: Teruel
authors.forename: Francisco José
authors.initials: FJ
authors.lastname: Aranda
authors.forename: Antonio
authors.initials: A
authors.lastname: Ortiz
authors.forename: Ángeles
authors.initials: Á
authors.lastname: Manresa
authors.forename: Ruth
authors.initials: R
authors.lastname: Ferrer
authors.forename: Ana M
authors.initials: AM
authors.lastname: Marqués
mesh_terms: D016475:3T3 Cells / Q000187:drug effects; D000818:Animals; D001688:Biological Products / Q000302:isolation & purification / Q000494:pharmacology*; D018938:Caco-2 Cells; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival; D002478:Cells, Cultured; D004912:Erythrocytes / Q000187:drug effects / Q000473:pathology; D006461:Hemolysis; D006801:Humans; D015603:Keratinocytes / Q000187:drug effects; D051379:Mice; D011817:Rabbits; D045249:Sphingobacterium / Q000378:metabolism*; D013501:Surface-Active Agents / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D001688:Biological Products; D013501:Surface-Active Agents
keywords: 3T3 fibroblasts; Apoptosis; Biosurfactants; Caco-2 cells; HaCaT keratinocytes; Haemolysis