title: In vitro ALP and osteocalcin gene expression analysis and in vivo biocompatibility of N-methylene phosphonic chitosan nanofibers for bone regeneration.
abstract: Most polymeric nanofibers used for bone tissue engineering lack adequate functional groups for bioactivity. This study explores the potential of nanofibers of phosphate functionalized derivative of chitosan-N-methylene phosphonic chitosan (NMPC) for bone tissue engineering. Nanofibers were fabricated by electrospinning of NMPC/PVA blend solutions. NMPC/PVA nanofibers exhibited 172% higher viability of MG-63 cells compared to pure PVA nanofibers. ALP and Collagen type I genes revealed higher expression in NMPC nanofibers on day 3 whereas osteocalcin gene was expressed on day 7. In rabbit tibial defects, NMPC based electrospun graft showed presence of no adverse tissue reaction by histological examination while radiological examination suggested acceleration of bone healing by 300% compared to defects without any scaffold. Thus it is concluded NMPC based nanofibers may have potential for bone grafting applications.
authors.affiliation: School of Medical Science and Technology, Indian Institute of Technology Kharagpur, Kharagpur, West Bengal 721302, India.
authors.forename: Pallab
authors.initials: P
authors.lastname: Datta
authors.forename: Paulomi
authors.initials: P
authors.lastname: Ghosh
authors.forename: Kuntal
authors.initials: K
authors.lastname: Ghosh
authors.forename: Pritiprasanna
authors.initials: P
authors.lastname: Maity
authors.forename: Sintu Kumar
authors.initials: SK
authors.lastname: Samanta
authors.forename: Sudip Kumar
authors.initials: SK
authors.lastname: Ghosh
authors.forename: Pradeep Kumar Das
authors.initials: PK
authors.lastname: Mohapatra
authors.forename: Jyotirmoy
authors.initials: J
authors.lastname: Chatterjee
authors.forename: Santanu
authors.initials: S
authors.lastname: Dhara
mesh_terms: D000469:Alkaline Phosphatase / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D001861:Bone Regeneration / Q000187:drug effects* / Q000502:physiology; D002478:Cells, Cultured; D048271:Chitosan / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use; D004353:Drug Evaluation, Preclinical; D015870:Gene Expression / Q000187:drug effects; D020869:Gene Expression Profiling; D006801:Humans; D008297:Male; D008422:Materials Testing; D057139:Nanofibers / Q000737:chemistry / Q000627:therapeutic use; D015675:Osteocalcin / Q000235:genetics* / Q000378:metabolism; D011817:Rabbits
chemical_list: C549127:N-methylene phosphonic chitosan; D015675:Osteocalcin; D048271:Chitosan; D000469:Alkaline Phosphatase