title: Characteristics of free endoglucanase and glycosidases multienzyme complex from Fusarium verticillioides.
abstract: A novel multienzyme complex, E1C, and a free endoglucanase, E2 (GH5), from Fusarium verticillioides were purified. The E1C contained two endoglucanases (GH6 and GH10), one cellobiohydrolase (GH7) and one xylanase (GH10). Maximum activity was observed at 80 °C for both enzymes and they were thermostable at 50 and 60 °C. The activation energies for E1C and E2 were 21.3 and 27.5 kJ/mol, respectively. The KM for E1C was 10.25 g/L while for E2 was 6.58 g/L. Both E1C and E2 were activated by Mn(2+) and CoCl2 while they were inhibited by SDS, CuSO4, FeCl3, AgNO4, ZnSO4 and HgCl2. E1C and E2 presented endo-β-1,3-1,4-glucanase activity. E1C presented crescent activity towards cellopentaose, cellotetraose and cellotriose. E2 hydrolyzed the substrates cellopentaose, cellotetraose and cellotriose with the same efficiency. E1C showed a higher stability and a better hydrolysis performance than E2, suggesting advantages resulting from the physical interaction between proteins.
authors.affiliation: Departamento de Bioquímica e Biologia Molecular, Universidade Federal de Viçosa, Viçosa, MG 36570-000, Brazil.
authors.forename: Maíra N
authors.initials: MN
authors.lastname: de Almeida
authors.forename: Daniel L
authors.initials: DL
authors.lastname: Falkoski
authors.forename: Valéria M
authors.initials: VM
authors.lastname: Guimarães
authors.forename: Humberto Josué de O
authors.initials: HJ
authors.lastname: Ramos
authors.forename: Evan M
authors.initials: EM
authors.lastname: Visser
authors.forename: Gabriela P
authors.initials: GP
authors.lastname: Maitan-Alfenas
authors.forename: Sebastião T
authors.initials: ST
authors.lastname: de Rezende
mesh_terms: D000595:Amino Acid Sequence; D002480:Cellulase / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000378:metabolism*; D002482:Cellulose / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005670:Fusarium / Q000201:enzymology*; D006026:Glycoside Hydrolases / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009097:Multienzyme Complexes / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000378:metabolism*; D013379:Substrate Specificity; D013696:Temperature
chemical_list: D004791:Enzyme Inhibitors; D009097:Multienzyme Complexes; D002482:Cellulose; D006026:Glycoside Hydrolases; D002480:Cellulase
keywords: Cellobiohydrolase; Endoglucanase; Fusarium verticillioides; Multienzyme complex; Xylanase