title: The internalization of fluorescence-labeled PLA nanoparticles by macrophages.
abstract: Rhodamine B (RhB)-labeled PLA nanoparticles were prepared through surface grafting copolymerization of glycidyl methacrylate (GMA) onto PLA nanoparticles during the emulsion/evaporation process. RhB firstly interacts with sodium dodecyl sulfate (SDS) through electrostatic interaction to form hydrophobic complex (SDS-RhB). Due to the high-affinity of SDS-RhB with GMA, hydrophilic RhB can be successfully combined into PLA nanoparticles. The internalization of RhB-labeled PLA nanoparticles by macrophages was investigated with fluorescence microscope technology. The effects of the PLA nanoparticle surface nature and size on the internalization were investigated. The results indicate that the PLA particles smaller than 200 nm can avoid the uptake of phagocytosis. The bigger PLA particles (300 nm) with polyethylene glycol (PEG) surface showed less internalization by macrophage compared with those with poly(ethylene oxide-propylene oxide) copolymer (F127) or poly(vinyl alcohol) (PVA) surface. The "stealth" function of PEG on the PLA nanoparticles from internalization of macrophages due to the low protein adsorption is revealed by electrochemical impedance technology.
authors.affiliation: College of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou 225002, PR China.
authors.forename: Fengjuan
authors.initials: F
authors.lastname: Li
authors.forename: Aiping
authors.initials: A
authors.lastname: Zhu
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Song
authors.forename: Lijun
authors.initials: L
authors.lastname: Ji
authors.forename: Juan
authors.initials: J
authors.lastname: Wang
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D017097:Electric Impedance; D004566:Electrodes; D004852:Epoxy Compounds / Q000737:chemistry; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000008:administration & dosage* / Q000737:chemistry; D019344:Lactic Acid / Q000008:administration & dosage* / Q000737:chemistry; D008264:Macrophages / Q000502:physiology*; D008689:Methacrylates / Q000737:chemistry; D051379:Mice; D053758:Nanoparticles / Q000008:administration & dosage* / Q000737:chemistry; D010316:Particle Size; D010587:Phagocytosis; D011091:Polyesters; D011108:Polymers / Q000008:administration & dosage* / Q000737:chemistry; D012235:Rhodamines / Q000008:administration & dosage* / Q000737:chemistry; D012710:Serum Albumin, Bovine / Q000737:chemistry
chemical_list: D004852:Epoxy Compounds; D005456:Fluorescent Dyes; D008689:Methacrylates; D011091:Polyesters; D011108:Polymers; D012235:Rhodamines; D012710:Serum Albumin, Bovine; D019344:Lactic Acid; C033616:poly(lactide); C029773:rhodamine B; C007870:glycidyl methacrylate
keywords: Electrochemical impedance; Macrophages internalization; RhB-labeled PLA nanoparticles; Surface grafting copolymerization