title: Silicon chips detect intracellular pressure changes in living cells.
abstract: The ability to measure pressure changes inside different components of a living cell is important, because it offers an alternative way to study fundamental processes that involve cell deformation. Most current techniques such as pipette aspiration, optical interferometry or external pressure probes use either indirect measurement methods or approaches that can damage the cell membrane. Here we show that a silicon chip small enough to be internalized into a living cell can be used to detect pressure changes inside the cell. The chip, which consists of two membranes separated by a vacuum gap to form a Fabry-Pérot resonator, detects pressure changes that can be quantified from the intensity of the reflected light. Using this chip, we show that extracellular hydrostatic pressure is transmitted into HeLa cells and that these cells can endure hypo-osmotic stress without significantly increasing their intracellular hydrostatic pressure.
authors.affiliation: Instituto de Microelectrónica de Barcelona, IMB-CNM (CSIC), Esfera UAB, Campus UAB, 08193, Cerdanyola, Barcelona, Spain.
authors.forename: Rodrigo
authors.initials: R
authors.lastname: Gómez-Martínez
authors.forename: Alberto M
authors.initials: AM
authors.lastname: Hernández-Pinto
authors.forename: Marta
authors.initials: M
authors.lastname: Duch
authors.forename: Patricia
authors.initials: P
authors.lastname: Vázquez
authors.forename: Kirill
authors.initials: K
authors.lastname: Zinoviev
authors.forename: Enrique J
authors.initials: EJ
authors.lastname: de la Rosa
authors.forename: Jaume
authors.initials: J
authors.lastname: Esteve
authors.forename: Teresa
authors.initials: T
authors.lastname: Suárez
authors.forename: José A
authors.initials: JA
authors.lastname: Plaza
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation*; D004867:Equipment Design; D006367:HeLa Cells; D006801:Humans; D042541:Intracellular Space / Q000737:chemistry; D056656:Lab-On-A-Chip Devices; D036103:Nanotechnology / Q000295:instrumentation; D011312:Pressure; D012825:Silicon / Q000737:chemistry
chemical_list: D012825:Silicon