title: Two-step protocol for isolation and culture of cardiospheres.
abstract: Cardiac progenitor cells (CPC) are a unique pool of progenitor cells residing in the heart that play an important role in cardiac homeostasis and physiological cardiovascular cell turnover during acute myocardial infarction (MI). Transplanting CPC into the heart has shown promise in two recent clinical trials of cardiac repair (SCIPIO & CADUCEUS). CSCs were originally isolated directly from enzymatically digested hearts followed by cell sorting using stem cell markers. However, long exposure to enzymatic digestion can affect the integrity of stem cell markers on the cell surface and also compromise stem cell function. Here, we describe a two-step procedure in which a large number of intact cardiac progenitor cells can be purified from small amount of heart tissue.
authors.affiliation: Division of Cardiovascular Disease, Department of Internal Medicine, College of Medicine, University of Cincinnati, Cincinnati, OH, USA.
authors.forename: Lijuan
authors.initials: L
authors.lastname: Chen
authors.forename: Yaohua
authors.initials: Y
authors.lastname: Pan
authors.forename: Lan
authors.initials: L
authors.lastname: Zhang
authors.forename: Yingjie
authors.initials: Y
authors.lastname: Wang
authors.forename: Neal
authors.initials: N
authors.lastname: Weintraub
authors.forename: Yaoliang
authors.initials: Y
authors.lastname: Tang
mesh_terms: D000818:Animals; D000950:Antigens, Ly / Q000378:metabolism; D002454:Cell Differentiation; D049109:Cell Proliferation; D002469:Cell Separation / Q000379:methods*; D002478:Cells, Cultured; D005434:Flow Cytometry; D006321:Heart / Q000254:growth & development*; D008565:Membrane Proteins / Q000378:metabolism; D051379:Mice; D032383:Myocytes, Cardiac / Q000166:cytology* / Q000378:metabolism; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism
chemical_list: D000950:Antigens, Ly; C074650:Ly6a protein, mouse; D008565:Membrane Proteins