title: [Biodistribution of the recombinant heat shock protein rhHsp70 in intracranial C6 glioma models in Wistar rats and subcutaneos B16/F10 melanoma in C57BL/6 mice].
abstract: For the first time, the biodistribution of recombinant heat shock protein in rhHsp70 rats with grafted intracranial C6 glioma was evaluated. It was assessed using the fluorescent antibody accumulation chaperone rhHsp70 conjugated with fluorochrome Alexa Fluor 555 in tumor cells by intratumoral or intravenous administration. Assessment of the distribution and accumulation of labeled protein was carried out on the model of subcutaneous B16/F10 melanoma in C57BL/6 mice with the use of single-photon emission computer tomography. After 60 minutes after intravenous administration rhHsp70-I123 (20 MBq, 5 mg chaperone) accumulation of the drug mainly in the liver and tumor tissue was showed. The coefficient of the differential accumulation of the labeled protein KDN(tumor/background) was 3.14. It was turned out that comparing the level of fixation of rhHsp70-I123 in the liver and the tumor KDN(tumor/ liver) = 0.76. After 24 hours from the time of injection of rhHsp70-I123 it was observed increase the level of fixation of the labeled protein in the liver and melanoma: KDN(tumor/background) = 3.43; KDN(tumor/liver = 0.78.
authors.forename: M A
authors.initials: MA
authors.lastname: Shevtsov
authors.forename: S V
authors.initials: SV
authors.lastname: Shatik
authors.lastname: Tokarev
authors.forename: M I
authors.initials: MI
authors.lastname: Mostova
authors.forename: L A
authors.initials: LA
authors.lastname: Tiutin
authors.forename: N V
authors.initials: NV
authors.lastname: Bychkova
authors.forename: A L
authors.initials: AL
authors.lastname: Mikhrina
authors.forename: K V
authors.initials: KV
authors.lastname: Onokhin
authors.forename: D A
authors.initials: DA
authors.lastname: Meshalkina
authors.forename: I V
authors.initials: IV
authors.lastname: Romanova
authors.forename: O N
authors.initials: ON
authors.lastname: Savchenko
authors.forename: A A
authors.initials: AA
authors.lastname: Ivanova
authors.forename: S V
authors.initials: SV
authors.lastname: Abkin
authors.forename: V A
authors.initials: VA
authors.lastname: Kharatian
authors.forename: I V
authors.initials: IV
authors.lastname: Guzhova
authors.forename: B A
authors.initials: BA
authors.lastname: Margulis
mesh_terms: D000818:Animals; D001932:Brain Neoplasms / Q000378:metabolism*; D005456:Fluorescent Dyes; D005910:Glioma / Q000378:metabolism*; D018840:HSP70 Heat-Shock Proteins / Q000008:administration & dosage / Q000378:metabolism* / Q000493:pharmacokinetics; D015552:Injections, Intralesional; D007275:Injections, Intravenous; D007457:Iodine Radioisotopes; D008099:Liver / Q000378:metabolism*; D008297:Male; D008546:Melanoma, Experimental / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D009374:Neoplasms, Experimental / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012878:Skin Neoplasms / Q000378:metabolism*; D013997:Time Factors; D014018:Tissue Distribution; D015899:Tomography, Emission-Computed, Single-Photon
chemical_list: D005456:Fluorescent Dyes; D018840:HSP70 Heat-Shock Proteins; D007457:Iodine Radioisotopes