title: Role of CPI-17 in restoring skin homoeostasis in cutaneous field of cancerization: effects of topical application of a film-forming medical device containing photolyase and UV filters.
abstract: Cutaneous field of cancerization (CFC) is caused in part by the carcinogenic effect of the cyclobutane pyrimidine dimers CPD and 6-4 photoproducts (6-4PPs). Photoreactivation is carried out by photolyases which specifically recognize and repair both photoproducts. The study evaluates the molecular effects of topical application of a film-forming medical device containing photolyase and UV filters on the precancerous field in AK from seven patients. Skin improvement after treatment was confirmed in all patients by histopathological and molecular assessment. A gene set analysis showed that skin recovery was associated with biological processes involved in tissue homoeostasis and cell maintenance. The CFC response was associated with over-expression of the CPI-17 gene, and a dependence on the initial expression level was observed (P = 0.001). Low CPI-17 levels were directly associated with pro-inflammatory genes such as TNF (P = 0.012) and IL-1B (P = 0.07). Our results suggest a role for CPI-17 in restoring skin homoeostasis in CFC lesions.
authors.affiliation: Melanoma Unit, Biochemistry and Molecular Genetics Service, Hospital Clinic of Barcelona, IDIBAPS, Barcelona, Spain; CIBER de Enfermedades Raras, Instituto de Salud Carlos III, Barcelona, Spain.
authors.forename: Joan Anton
authors.initials: JA
authors.lastname: Puig-Butillé
authors.forename: Josep
authors.initials: J
authors.lastname: Malvehy
authors.forename: Miriam
authors.initials: M
authors.lastname: Potrony
authors.forename: Carles
authors.initials: C
authors.lastname: Trullas
authors.forename: Francisco
authors.initials: F
authors.lastname: Garcia-García
authors.forename: Joaquin
authors.initials: J
authors.lastname: Dopazo
authors.forename: Susana
authors.initials: S
authors.lastname: Puig
mesh_terms: D000287:Administration, Topical; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001706:Biopsy; D004255:Deoxyribodipyrimidine Photo-Lyase / Q000008:administration & dosage* / Q000737:chemistry; D005260:Female; D020869:Gene Expression Profiling; D015971:Gene Expression Regulation, Enzymologic; D015972:Gene Expression Regulation, Neoplastic; D006706:Homeostasis; D006801:Humans; D007249:Inflammation; D047908:Intracellular Signaling Peptides and Proteins; D008081:Liposomes / Q000008:administration & dosage*; D008297:Male; D008875:Middle Aged; D009124:Muscle Proteins; D010641:Phenotype; D010749:Phosphoprotein Phosphatases / Q000502:physiology*; D017382:Reactive Oxygen Species; D012867:Skin / Q000378:metabolism*; D012878:Skin Neoplasms / Q000378:metabolism*; D014466:Ultraviolet Rays
chemical_list: D047908:Intracellular Signaling Peptides and Proteins; D008081:Liposomes; D009124:Muscle Proteins; C107507:PPP1R14A protein, human; D017382:Reactive Oxygen Species; D010749:Phosphoprotein Phosphatases; D004255:Deoxyribodipyrimidine Photo-Lyase