title: [Use of immunological and molecular biological methods to diagnose cerebral toxoplasmosis in HIV infection].
abstract: Cerebral toxoplasmosis is one of the leading causes of neurologic diseases with high mortality rates in patients with HIV infection. Invasion was difficult to diagnose for a number of objective reasons. The objective of the investigation was to determine the clinical sensitivity of different laboratory techniques as both a single study and their various combinations to verify the diagnosis of cerebral toxoplasmosis in HIV-infected patients. Blood and cerebrospinal fluid were tested in 51 patients with Stage 4B HIV infection (AIDS) with the verified diagnosis of cerebral toxoplasmosis. Separate determination of specific antibodies of IgG, IgM, IgA and toxoplasma DNA in the blood and cerebrospinal fluid was shown to have an insufficient clinical sensitivity (37.3-68.6%). The benefits of various combinations of immunological and molecular biological assays enhancing the diagnostic efficiency up to 76.5-96.1% are demonstrated.
authors.forename: E V
authors.initials: EV
authors.lastname: Gubareva
authors.forename: D B
authors.initials: DB
authors.lastname: Goncharov
authors.forename: É A
authors.initials: ÉA
authors.lastname: Domonova
authors.forename: O Iu
authors.initials: OIu
authors.lastname: Sil'veĭstrova
authors.forename: A B
authors.initials: AB
authors.lastname: Peregudova
authors.forename: O A
authors.initials: OA
authors.lastname: Tishkevich
authors.forename: E S
authors.initials: ES
authors.lastname: Ievleva
authors.forename: N V
authors.initials: NV
authors.lastname: Kobets
authors.forename: O Iu
authors.initials: OIu
authors.lastname: Shipulina
mesh_terms: D000328:Adult; D000913:Antibodies, Protozoan / Q000097:blood* / Q000134:cerebrospinal fluid; D001921:Brain / Q000469:parasitology / Q000473:pathology* / Q000821:virology; D060085:Coinfection; D016054:DNA, Protozoan / Q000097:blood* / Q000134:cerebrospinal fluid; D018450:Disease Progression; D005260:Female; D006678:HIV; D015658:HIV Infections / Q000097:blood / Q000134:cerebrospinal fluid / Q000473:pathology* / Q000821:virology; D006801:Humans; D007118:Immunoassay; D007070:Immunoglobulin A / Q000097:blood / Q000134:cerebrospinal fluid; D007074:Immunoglobulin G / Q000097:blood / Q000134:cerebrospinal fluid; D007075:Immunoglobulin M / Q000097:blood / Q000134:cerebrospinal fluid; D008279:Magnetic Resonance Imaging; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D014122:Toxoplasma / Q000276:immunology / Q000302:isolation & purification*; D016781:Toxoplasmosis, Cerebral / Q000097:blood / Q000134:cerebrospinal fluid / Q000175:diagnosis* / Q000469:parasitology
chemical_list: D000913:Antibodies, Protozoan; D016054:DNA, Protozoan; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M