title: In vitro cytokine expression by peripheral mononuclear cells in herbal drug-induced skin eruption.
abstract: Herbal medicine is widely used worldwide and is associated with side-effects such as skin eruptions. Herbal drugs are often produced by combining multiple crude drugs, mostly of plant origin. Determining which medi-cinal plants are associated with the herbal drugs that induce skin eruptions can therefore be difficult. This study investigated mRNA expression of several cytokines in peripheral mononuclear cells (PBMCs) from two patients with herbal drug-induced skin eruptions; one reacted to keishi-bukuryo-gan (KBG), composed of 5 medicinal plants, and the other patient reacted to senna. PBMCs (1×106) from the 2 patients were cultured for 24 h with the supernatant from the medicinal plants from KBG or senna in various concentrations, and a reverse transcription-polymerase chain reaction (RT-PCR) analysis was performed. A high mRNA level of interleukin (IL)-4 and IL-5 was detected in PBMCs stimulated by KBG and two of its components. Senna stimulated a high level of IL-4 and IL-5 mRNA levels in PBMCs from patient with senna-induced drug reaction.
authors.affiliation: Department of Dermatology, Graduate School of Medicine, University of Toyama, Sugitani, Toyama, Japan.
authors.forename: Osamu
authors.initials: O
authors.lastname: Norisugi
authors.forename: Yoko
authors.initials: Y
authors.lastname: Yoshihisa
authors.forename: Kyoko
authors.initials: K
authors.lastname: Shimizu
authors.forename: Tadamichi
authors.initials: T
authors.lastname: Shimizu
mesh_terms: D000369:Aged, 80 and over; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002935:Cinnamomum zeylanicum; D003875:Drug Eruptions / Q000209:etiology / Q000378:metabolism*; D004365:Drugs, Chinese Herbal / Q000009:adverse effects / Q000737:chemistry / Q000494:pharmacology*; D005260:Female; D015870:Gene Expression / Q000187:drug effects*; D006801:Humans; D015847:Interleukin-4 / Q000235:genetics; D015848:Interleukin-5 / Q000235:genetics; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000378:metabolism*; D008875:Middle Aged; D029596:Paeonia; D012333:RNA, Messenger / Q000378:metabolism*; D012676:Senna Extract / Q000009:adverse effects / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D004365:Drugs, Chinese Herbal; D015848:Interleukin-5; D012333:RNA, Messenger; C532132:cinnamomi cortex; C054593:keishibukuryogan; C038881:moutan cortex; D015847:Interleukin-4; D012676:Senna Extract