title: Copper-resistant bacteria enhance plant growth and copper phytoextraction.
abstract: In this study, we investigated the role of rhizospheric bacteria in solubilizing soil copper (Cu) and promoting plant growth. The Cu-resistant bacterium DGS6 was isolated from a natural Cu-contaminated soil and was identified as Pseudomonas sp. DGS6. This isolate solubilized Cu in Cu-contaminated soil and stimulated root elongation of maize and sunflower. Maize was more sensitive to inoculation with DGS6 than was sunflower and exhibited greater root elongation. In pot experiment, inoculation with DGS6 increased the shoot dry weight of maize by 49% and sunflower by 34%, and increased the root dry weight of maize by 85% and sunflower by 45%. Although the concentrations of Cu in inoculated and non-inoculated seedlings did not differ significantly, the total accumulation of Cu in the plants increased after inoculation. DGS6 showed a high ability to solubilize P and produce iron-chelating siderophores, as well as significantly improved the accumulation of P and Fe in both maize and sunflower shoots. In addition, DGS6 produced indole-3-acetic acid (IAA) and ACC deaminase, which suggests that it may modulate ethylene levels in plants. The bacterial strain DGS6 could be a good candidate for re-vegetation of Cu-contaminated sites. Supplemental materials are available for this article. Go to the publisher's online edition of International Journal of Phytoremediation to view the supplemental file.
authors.affiliation: Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou, China.
authors.forename: Renxiu
authors.initials: R
authors.lastname: Yang
authors.forename: Chunling
authors.initials: C
authors.lastname: Luo
authors.forename: Yahua
authors.initials: Y
authors.lastname: Chen
authors.forename: Guiping
authors.initials: G
authors.lastname: Wang
authors.forename: Yue
authors.initials: Y
authors.lastname: Xu
authors.forename: Zhenguo
authors.initials: Z
authors.lastname: Shen
mesh_terms: D001673:Biodegradation, Environmental; D018533:Biomass; D019755:Carbon-Carbon Lyases / Q000378:metabolism; D002681:China; D003300:Copper / Q000378:metabolism*; D005030:Ethylenes / Q000378:metabolism; D006368:Helianthus / Q000254:growth & development / Q000382:microbiology* / Q000502:physiology; D007210:Indoleacetic Acids / Q000378:metabolism; D007501:Iron / Q000378:metabolism; D008906:Mining; D010758:Phosphorus / Q000378:metabolism; D018517:Plant Roots / Q000254:growth & development / Q000382:microbiology / Q000502:physiology; D018520:Plant Shoots / Q000254:growth & development / Q000382:microbiology / Q000502:physiology; D011549:Pseudomonas / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D058441:Rhizosphere; D036226:Seedlings / Q000378:metabolism; D017262:Siderophores / Q000032:analysis / Q000378:metabolism; D012987:Soil / Q000737:chemistry; D012989:Soil Pollutants / Q000378:metabolism; D003313:Zea mays / Q000254:growth & development / Q000382:microbiology* / Q000502:physiology
chemical_list: D005030:Ethylenes; D007210:Indoleacetic Acids; D017262:Siderophores; D012987:Soil; D012989:Soil Pollutants; D010758:Phosphorus; C030737:indoleacetic acid; D003300:Copper; C036216:ethylene; D007501:Iron; C033634:1-aminocyclopropane-1-carboxylate deaminase; D019755:Carbon-Carbon Lyases