title: [Antimicrobial activity of stable silver nanoparticles of a certain size].
abstract: Conditions for obtaining stable silver nanoparticles smaller than 10 nm were developed using a binary stabilizer polyvinylpyrrolidone/sodium dodecylsulphate in optimal ratio. Optical spectra, morphology and dependence of size of the nanoparticles on the amount of reducing agent were studied. Colloidal solutions of nanosilver showed a high bactericidal activity against strains of Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa, and fungicidal activity against Candida albicans. The mechanism of action of nanosized silver on microbial cell was examined by laser scanning confocal microscope using fluorescent label. First step of antimicrobial effect on microorganisms was membrane damage and penetration of silver nanoparticles into the cell. Prolonged stability of nanoparticles and their antimicrobial activity over the past two years were showed.
authors.forename: Iu P
authors.initials: IuP
authors.lastname: Mukha
authors.forename: A M
authors.initials: AM
authors.lastname: Eremenko
authors.forename: N P
authors.initials: NP
authors.lastname: Smirnova
authors.forename: A I
authors.initials: AI
authors.lastname: Mikhienkova
authors.forename: G I
authors.initials: GI
authors.lastname: Korchak
authors.forename: V F
authors.initials: VF
authors.lastname: Gorchev
authors.forename: A Iu
authors.initials: AIu
authors.lastname: Chunikhin
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D000935:Antifungal Agents / Q000737:chemistry / Q000494:pharmacology*; D002176:Candida albicans / Q000187:drug effects / Q000254:growth & development; D002462:Cell Membrane / Q000187:drug effects*; D003102:Colloids; D004926:Escherichia coli / Q000187:drug effects / Q000254:growth & development; D005456:Fluorescent Dyes; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D008826:Microbial Sensitivity Tests; D050296:Microbial Viability / Q000187:drug effects; D018613:Microscopy, Confocal; D010316:Particle Size; D011205:Povidone / Q000737:chemistry; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000254:growth & development; D012834:Silver / Q000737:chemistry / Q000494:pharmacology*; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry; D013211:Staphylococcus aureus / Q000187:drug effects / Q000254:growth & development
chemical_list: D000900:Anti-Bacterial Agents; D000935:Antifungal Agents; D003102:Colloids; D005456:Fluorescent Dyes; D012967:Sodium Dodecyl Sulfate; D012834:Silver; D011205:Povidone