title: Sublethal effects of the flame retardant intermediate hexachlorocyclopentadiene (HCCPD) on the gene transcription and protein activity of Daphnia magna.
abstract: Hexachlorocyclopentadiene (HCCPD) is a chlorinated chemical of high production volume used as an intermediate in the production of flame retardants. HCCPD may be released to the environment during production, use, and as a result of product degradation. The objectives of this study were to evaluate sublethal effects of HCCPD exposure to Daphnia magna at environmentally relevant concentrations (0.0138-13.8 μg/L) using genomic tools (microarray and qPCR), enzyme activities, and life-history endpoints (survival, reproduction, and growth). In chronic exposures, no differences were observed in life-history endpoints (survival, time of first brood, time of first molt, molt frequency, number of neonates, and body length) between exposed organisms and controls. Microarray analyses indicated significant differential genomic transcription for 46 genes (p-value ≤ 0.05 and fold-change>2). Five identified genes were related to metabolic functions. Enzyme activities of α-amylase and trypsin, selected based on transcriptional responses, were evaluated in D. magna. Although trypsin activity was similar between treatments and controls, the activity of α-amylase significantly decreased with increasing HCCPD concentrations. On the chemical level, instability of HCCPD was observed in spiked culture media, most probably due to photolysis and biodegradation. HCCPD was not detected in surface water samples collected upstream and at the point of discharge of a major wastewater treatment plant effluent. Environmentally, rapid degradation of HCCPD could be outdone by its continuous release into aquatic ecosystems in specific areas of concern (e.g., vicinity of industries and hazardous sites). Toxicity results from this study highlight the use of genomics in the identification of biomarkers and help advance the science, and potential use, of multi-level biological approaches for environmental risk assessment.
authors.affiliation: Environment Canada, Centre Saint-Laurent, 105 McGill Street, Montreal, QC, Canada H2Y 2E7. magali.houde@ec.gc.ca
authors.forename: Magali
authors.initials: M
authors.lastname: Houde
authors.forename: Barbara
authors.initials: B
authors.lastname: Carter
authors.forename: Mélanie
authors.initials: M
authors.lastname: Douville
mesh_terms: D000818:Animals; D003621:Daphnia / Q000187:drug effects* / Q000235:genetics; D004789:Enzyme Activation / Q000187:drug effects; D005411:Flame Retardants / Q000633:toxicity*; D005786:Gene Expression Regulation / Q000187:drug effects*; D006843:Hydrocarbons, Chlorinated / Q000633:toxicity*; D016019:Survival Analysis; D014874:Water Pollutants, Chemical / Q000633:toxicity; D000516:alpha-Amylases / Q000378:metabolism
chemical_list: D005411:Flame Retardants; D006843:Hydrocarbons, Chlorinated; D014874:Water Pollutants, Chemical; D000516:alpha-Amylases; C016488:hexachlorocyclopentadiene
keywords: Daphnia magna; Enzyme activity; Gene transcription; Hexachlorocyclopentadiene; Life-history endpoints