title: [Aminoguanidine suppresses methylglyoxal-mediated oxygen-glucose deprivation injury in human brain microvascular endothelial cells].
abstract: OBJECTIVE
  To evaluate the effects of aminoguanidine on methylglyoxal-mediated oxygen-glucose deprivation (OGD) injury in the cultured human brain microvascular endothelial cells (HBMEC).
  
  
  METHODS
  Cultured HBMEC cells were pretreated with methylglyoxal before oxygen-glucose deprivation injury. Cell vitality was determined by MTT method, cell mortality was assessed by LDH release method, cell apoptosis was examined by Annexin V/PI formation method, and the advanced glycation end products (AGEs) were detected by Western-blot.
  
  
  RESULTS
  Methylglyoxal induced HBMEC injury in a dose-dependent manner. At 2 mmol/L of methylglyoxal, the cell viability was 56.1% when methylglyoxal-pretreated cells exposed to oxygen-glucose deprivation, the cell inhibition rate was 90.0%. Aminoguanidine (1 mmol/L) inhibited methylglyoxal and OGD induced LDH release and Annexin V/PI formation. Furthermore, aminoguanidine (1 mmol/L) also decreased advanced glycation end products (AGEs) formation induced by methylglyoxal and oxygen-glucose deprivation.
  
  
  CONCLUSIONS
  Aminoguanidine protected methylglyoxal mediated-oxygen-glucose deprivation injury in the cultured HBMEC, which may be associated with anti-glycation activity.
authors.affiliation: The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310009, China.
authors.forename: Wenlu
authors.initials: W
authors.lastname: Li
authors.forename: Quan
authors.initials: Q
authors.lastname: Hu
authors.forename: Xia
authors.initials: X
authors.lastname: Ren
authors.forename: Ping
authors.initials: P
authors.lastname: He
authors.forename: Huimin
authors.initials: H
authors.lastname: Xu
authors.forename: Haibin
authors.initials: H
authors.lastname: Dai
authors.forename: Zhong
authors.initials: Z
authors.lastname: Chen
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D015687:Cell Hypoxia / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004336:Drug Antagonism; D042783:Endothelial Cells / Q000187:drug effects / Q000378:metabolism / Q000473:pathology*; D004730:Endothelium, Vascular / Q000166:cytology; D017127:Glycation End Products, Advanced / Q000378:metabolism; D006146:Guanidines / Q000494:pharmacology*; D006801:Humans; D011765:Pyruvaldehyde / Q000494:pharmacology*
chemical_list: D017127:Glycation End Products, Advanced; D006146:Guanidines; D011765:Pyruvaldehyde; C004479:pimagedine