title: Effects of propofol on damage of rat intestinal epithelial cells induced by heat stress and lipopolysaccharides.
abstract: Gut-derived endotoxin and pathogenic bacteria have been proposed as important causative factors of morbidity and death during heat stroke. However, it is still unclear what kind of damage is induced by heat stress. In this study, the rat intestinal epithelial cell line (IEC-6) was treated with heat stress or a combination of heat stress and lipopolysaccharide (LPS). In addition, propofol, which plays an important role in anti-inflammation and organ protection, was applied to study its effects on cellular viability and apoptosis. Heat stress, LPS, or heat stress combined with LPS stimulation can all cause intestinal epithelial cell damage, including early apoptosis and subsequent necrosis. However, propofol can alleviate injuries caused by heat stress, LPS, or the combination of heat stress and LPS. Interestingly, propofol can only mitigate LPS-induced intestinal epithelial cell apoptosis, and has no protective role in heat-stress-induced apoptosis. This study developed a model that can mimic the intestinal heat stress environment. It demonstrates the effects on intestinal epithelial cell damage, and indicated that propofol could be used as a therapeutic drug for the treatment of heat-stress-induced intestinal injuries.
authors.affiliation: Southern Medical University, Nanfang Hospital, Department of Anesthesia, Guangzhou, China, Department of Anesthesia, Nanfang Hospital, Southern Medical University, Guangzhou, China.
authors.forename: J
authors.initials: J
authors.lastname: Tang
authors.forename: Y
authors.initials: Y
authors.lastname: Jiang
authors.forename: Y
authors.initials: Y
authors.lastname: Tang
authors.forename: B
authors.initials: B
authors.lastname: Chen
authors.forename: X
authors.initials: X
authors.lastname: Sun
authors.forename: L
authors.initials: L
authors.lastname: Su
authors.forename: Z
authors.initials: Z
authors.lastname: Liu
mesh_terms: D018686:Anesthetics, Intravenous / Q000494:pharmacology*; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000627:therapeutic use; D017209:Apoptosis / Q000187:drug effects; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D004847:Epithelial Cells / Q000187:drug effects*; D005562:Formazans; D018883:Heat Stroke / Q000150:complications* / Q000188:drug therapy; D018869:Heat-Shock Response / Q000187:drug effects; D007422:Intestines / Q000166:cytology / Q000382:microbiology / Q000473:pathology; D008070:Lipopolysaccharides / Q000633:toxicity; D009336:Necrosis; D015742:Propofol / Q000494:pharmacology*; D051381:Rats; D013778:Tetrazolium Salts
chemical_list: D018686:Anesthetics, Intravenous; D000893:Anti-Inflammatory Agents; D005562:Formazans; D008070:Lipopolysaccharides; D013778:Tetrazolium Salts; C079782:MTT formazan; D015742:Propofol