title: A novel electrochemical sensor for the analysis of β-agonists: the poly(acid chrome blue K)/graphene oxide-nafion/glassy carbon electrode.
abstract: A novel modified electrode was constructed by the electro-polymerization of 4,5-dihydroxy-3-[(2-hydroxy-5-sulfophenyl)azo]-2,7-naphthalenedisulfonic acid trisodium salt (acid chrome blue K (ACBK)) at a graphene oxide (GO)-nafion modified glassy carbon electrode (GCE). The characterization of an electrochemically synthesized poly-ACBK/GO-nafion film was investigated by electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV), atomic force microscopy (AFM) and scanning electron microscopy (SEM) techniques, and the results were interpreted and compared at each stage of the electrode construction. Electrochemical oxidation of eight β-agonists - clenbuterol, salbutamol, terbutaline, ractopamine, dopamine, dobutamine, adrenaline, and isoprenaline, was investigated by CV at the different electrodes. At the poly-ACBK/GO-nafion/GCE, the linear sweep voltammetry peak currents of the eight β-agonists increased linearly with their concentrations in the range of 1.0-36.0 ng mL(-1), respectively, and their corresponding limits of detection (LODs) were within the 0.58-1.46 ng mL(-1) range. This electrode showed satisfactory reproducibility and stability, and was used successfully for the quantitative analysis of clenbuterol in pork samples.
authors.affiliation: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China.
authors.forename: Xiaoyun
authors.initials: X
authors.lastname: Lin
authors.forename: Yongnian
authors.initials: Y
authors.lastname: Ni
authors.forename: Serge
authors.initials: S
authors.lastname: Kokot
mesh_terms: D000318:Adrenergic beta-Agonists / Q000032:analysis*; D000818:Animals; D001391:Azo Compounds / Q000737:chemistry; D002138:Calibration; D002244:Carbon / Q000737:chemistry; D002976:Clenbuterol / Q000032:analysis*; D058266:Dielectric Spectroscopy; D004563:Electrochemistry / Q000379:methods*; D004566:Electrodes; D005465:Fluorocarbon Polymers / Q000737:chemistry; D005506:Food Contamination / Q000032:analysis*; D006108:Graphite / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D057230:Limit of Detection; D008460:Meat / Q000032:analysis; D018625:Microscopy, Atomic Force; D008855:Microscopy, Electron, Scanning; D009282:Naphthalenesulfonates / Q000737:chemistry; D010087:Oxides / Q000737:chemistry; D015203:Reproducibility of Results; D013552:Swine
chemical_list: D000318:Adrenergic beta-Agonists; D001391:Azo Compounds; D005465:Fluorocarbon Polymers; D009282:Naphthalenesulfonates; D010087:Oxides; C508261:acid chrome blue K; C040402:perfluorosulfonic acid; D002244:Carbon; D006108:Graphite; D002976:Clenbuterol
keywords: Acid chrome blue K; Electro-polymerization; Graphene oxide-nafion film/GCE; Pork samples; Voltammetry; β-Agonists