title: Alginate-chitosan-PLGA composite microspheres induce both innate and adaptive immune response through parenteral immunization in fish.
abstract: Alginate-chitosan-PLGA composite microspheres encapsulating outer membrane protein antigen of Aeromonas hydrophila as an antigen carrier was explored for the first time in a fish model. This composite microsphere showed distinct advantages over the conventional PLGA microparticles in aspects of the high encapsulation efficiency due to the protein-friendly microenvironment created by the hydrophilic alginate-chitosan cores of the composite microspheres, preventing initial burst release and the elimination of lyophilizing process. The antibody responses significantly increased and persist up to 9 weeks in composite microspheres unlike that PLGA microsphere, native OMP and FIA adjuvant. Moreover, several innate immune parameters as respiratory burst, lysozyme and complement activity were significantly increased in both composite and PLGA microspheres up to 9 weeks than other treated groups. It also gives protection from A. hydrophila infection and brought some hope, for its application in replacement with conventional PLGA microparticle for antigen delivery in fish.
authors.affiliation: Fish Health Management Division, Central Institute of Freshwater Aquaculture, Kausalyaganga, Bhubaneswar 751 002, Orissa, India.
authors.forename: Truptimayee
authors.initials: T
authors.lastname: Behera
authors.forename: Priyabrat
authors.initials: P
authors.lastname: Swain
mesh_terms: D056704:Adaptive Immunity; D016980:Aeromonas hydrophila / Q000276:immunology*; D000464:Alginates / Q000737:chemistry*; D000818:Animals; D000907:Antibodies, Bacterial; D048271:Chitosan / Q000737:chemistry*; D003530:Cyprinidae; D005393:Fish Diseases / Q000382:microbiology / Q000517:prevention & control; D020723:Glucuronic Acid / Q000737:chemistry; D016905:Gram-Negative Bacterial Infections / Q000382:microbiology / Q000517:prevention & control / Q000662:veterinary*; D006603:Hexuronic Acids / Q000737:chemistry; D007113:Immunity, Innate; D019344:Lactic Acid / Q000737:chemistry*; D008863:Microspheres; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D050994:Voltage-Dependent Anion Channels
chemical_list: D000464:Alginates; D000907:Antibodies, Bacterial; D006603:Hexuronic Acids; D050994:Voltage-Dependent Anion Channels; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D020723:Glucuronic Acid; D048271:Chitosan
keywords: Aeromonas hydrophila; Alginate; Chitosan; Composite microspheres; PLGA