title: Effects of CO2 enrichment and nutrients supply intermittency on batch cultures of Isochrysis galbana.
abstract: Aiming at enhanced performance to increase economic feasibility of microalgae based processes, Isochrysis galbana was grown in three modes of cultivation: batch, intermittent fed batch and semi-continuous. The batch mode was conducted under two regimes of aeration: conventional aeration and CO2 enriched aeration (5% v/v in air). Increased biomass productivity without significant impact on lipid accumulation was observed for CO2 enriched aeration relatively to cultivation aerated with air only. The intermittent fed batch cultivation policy was proven to be useful for lipid accumulation, increasing the lipid content by 19.8%. However, the semi-continuous mode resulted in higher productivity due to increased biomass concentration; the biomass productivity reached 0.51 g/(Ld). Fluorescence measurements were performed; the calculated low electron transport rate showed the need to increase the irradiance. The results showed that I. galbana can be grown in semi-continuous condition at high levels of biomass productivity.
authors.affiliation: Escola de Química, Federal University of Rio de Janeiro, Av. Horácio Macedo 2030, Centro de Tecnologia, Bl. E, Sala 201, Rio de Janeiro, RJ 21941-909, Brazil. picardo@eq.ufrj.br
authors.forename: Marta C
authors.initials: MC
authors.lastname: Picardo
authors.forename: José Luiz
authors.initials: JL
authors.lastname: de Medeiros
authors.forename: Ofélia de Queiroz F
authors.initials: Ode Q
authors.lastname: Araújo
authors.forename: Ricardo Moreira
authors.initials: RM
authors.lastname: Chaloub
mesh_terms: D061249:Batch Cell Culture Techniques; D018533:Biomass; D002245:Carbon Dioxide / Q000737:chemistry*; D002734:Chlorophyll / Q000378:metabolism; D000077194:Chlorophyll A; D005240:Feasibility Studies; D005453:Fluorescence; D058087:Haptophyta / Q000166:cytology / Q000378:metabolism*
chemical_list: D002734:Chlorophyll; D002245:Carbon Dioxide; D000077194:Chlorophyll A
keywords: Batch operation; CO(2) enriched growth; Intermittent fed batch; Isochrysis galbana; Semi-continuous operation