title: Microglial activation: measurement of cytokines by flow cytometry.
abstract: Cytokine measurement is a prerequisite to understand the inflammatory state of the body. Quantitative analysis of cytokines by Western blotting and ELISA is a daunting task as these are time-consuming and error-prone protocols. With the advent of flow cytometry, the estimation of cytokines using the classical antigen-antibody reaction has become a popular choice with researchers/clinicians. Here, we describe a protocol for multiple cytokine analysis using flow cytometry.
authors.affiliation: Department of cellular and Molecular Neurosciences, National Brain Research Centre, Manesar, India.
authors.forename: Deepak Kumar
authors.initials: DK
authors.lastname: Kaushik
authors.forename: Anirban
authors.initials: A
authors.lastname: Basu
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002478:Cells, Cultured; D016207:Cytokines / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry; D051379:Mice; D017628:Microglia / Q000166:cytology / Q000378:metabolism*
chemical_list: D016207:Cytokines