title: Growth arrest specific 1 (GAS1) is abundantly expressed in the adult mouse central nervous system.
abstract: Growth arrest specific 1 (GAS1) is a pleiotropic protein that induces apoptosis and cell arrest in different tumors, but it is also involved in the development of the nervous system and other tissues and organs. This dual ability is likely caused by its capacity to interact both by inhibiting the intracellular signaling cascade induced by glial cell-line derived neurotrophic factor and by facilitating the activity of the sonic hedgehog pathway. The presence of GAS1 mRNA has been described in adult mouse brain, and here we corroborated this observation. We then proceeded to determine the distribution of the protein in the adult central nervous system (CNS). We detected, by western blot analysis, expression of GAS1 in olfactory bulb, caudate-putamen, cerebral cortex, hippocampus, mesencephalon, medulla oblongata, cerebellum, and cervical spinal cord. To more carefully map the expression of GAS1, we performed double-label immunohistochemistry and noticed expression of GAS1 in neurons in all brain areas examined. We also observed expression of GAS1 in astroglial cells, albeit the pattern of expression was more restricted than that seen in neurons. Briefly, in the present article, we report the widespread distribution and cellular localization of the GAS1 native protein in adult mammalian CNS.
authors.affiliation: Departamento de Fisiología, Biofísica y Neurociencias, Centro de Investigación y de Estudios Avanzados del IPN (NZ,EB,PV,PF-R,JS).
authors.forename: Natanael
authors.initials: N
authors.lastname: Zarco
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Bautista
authors.forename: Manola
authors.initials: M
authors.lastname: Cuéllar
authors.forename: Paula
authors.initials: P
authors.lastname: Vergara
authors.forename: Paola
authors.initials: P
authors.lastname: Flores-Rodriguez
authors.forename: Raúl
authors.initials: R
authors.lastname: Aguilar-Roblero
authors.forename: José
authors.initials: J
authors.lastname: Segovia
mesh_terms: D000818:Animals; D001921:Brain / Q000166:cytology / Q000378:metabolism*; D018797:Cell Cycle Proteins / Q000235:genetics* / Q000378:metabolism*; D058851:GPI-Linked Proteins / Q000235:genetics / Q000378:metabolism; D005786:Gene Expression Regulation; D008297:Male; D051379:Mice; D041681:NIH 3T3 Cells; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013116:Spinal Cord / Q000166:cytology / Q000378:metabolism*
chemical_list: D018797:Cell Cycle Proteins; D058851:GPI-Linked Proteins; C501180:Gas1 protein, mouse; D012333:RNA, Messenger
keywords: central nervous system; co-localization; glial cell-line derived neurotrophic factor; glial fibrillary acidic protein; growth arrest specific 1; neuronal nuclei; sonic hedgehog