title: Inkjet nanoinjection for high-thoughput chemiluminescence immunoassay on multicapillary glass plate.
abstract: We report a novel chemiluminescence diagnosis system for high-throughput human IgA detection by inkjet nanoinjection on a multicapillary glass plate. As proof-of-concept, microhole-based polydimethylsiloxane (PDMS) sheets were aligned on a multicapillary glass plate to form a microwell array as microreactors for enzyme-linked immunosorbent assay (ELISA). The multicapillary glass plate was utilized as a switch that controlled the holding/passing of the solution. Further, anti-IgA-labeled polystyrene (PS) microbeads was assembled into the microwell array, and an inkjet nanoinjection was specially used to distribute the sample and reagent solution for chemiluminescence ELISA, enabling high-throughput detection of human IgA. As a result, the performance of human IgA tests revealed a wider range for the calibration curve and a lower limit of detection (LOD) of 0.1 ng mL(-1) than the ELISA by a standard 96-well plate. The analysis time and reagent consumption were significantly decreased. The IgA concentrations in saliva samples were determined after 10000-fold dilution by the developed ELISA system showing comparable results by conventional immune assay with 96-wells. Thus, we believe that the inkjet nanoinjection for high-throughput chemiluminescence immunoassay on a multicapillary glass plate will be promising in disease diagnosis.
authors.affiliation: Department of Applied Chemistry, Graduate School of Urban Environmental Sciences, Tokyo Metropolitan University, Hachioji, Tokyo, Japan.
authors.forename: Fengming
authors.initials: F
authors.lastname: Chen
authors.forename: Sifeng
authors.initials: S
authors.lastname: Mao
authors.forename: Hulie
authors.initials: H
authors.lastname: Zeng
authors.forename: Shuhua
authors.initials: S
authors.lastname: Xue
authors.forename: Jianmin
authors.initials: J
authors.lastname: Yang
authors.forename: Hizuru
authors.initials: H
authors.lastname: Nakajima
authors.forename: Jin-Ming
authors.initials: JM
authors.lastname: Lin
authors.forename: Katsumi
authors.initials: K
authors.lastname: Uchiyama
mesh_terms: D000818:Animals; D002138:Calibration; D004129:Dimethylpolysiloxanes / Q000737:chemistry; D005898:Glass; D006735:Horseradish Peroxidase / Q000378:metabolism; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation*; D007070:Immunoglobulin A / Q000032:analysis / Q000097:blood; D007267:Injections; D007281:Ink; D008163:Luminescent Measurements / Q000295:instrumentation*; D036103:Nanotechnology / Q000295:instrumentation*; D012463:Saliva / Q000737:chemistry; D014835:Volatilization
chemical_list: D004129:Dimethylpolysiloxanes; D007070:Immunoglobulin A; C013830:baysilon; D006735:Horseradish Peroxidase