title: Algicidal activity of Salvia miltiorrhiza Bung on Microcystis aeruginosa--towards identification of algicidal substance and determination of inhibition mechanism.
abstract: The present study was to isolate and identify a potent algicidal compound from extract of Salvia miltiorrhiza and study the potential inhibition mechanism on Microcystis aeruginosa. Column chromatography and bioassay-guided fractionation methods were carried out to yield neo-przewaquinone A, which was identified by spectral analysis. The EC50 of neo-przewaquinone A on M. aeruginosa were 4.68 mg L(-1). In addition, neo-przewaquinone A showed relatively higher security on Chlorella pyrenoidosa and Scenedesmus obliquus, with the EC50 values of 14.78 and 10.37 mg L(-1), respectively. For the potential inhibition mechanisms, neo-przewaquinone A caused M. aeruginosa cells morphologic damage or lysis, increased malondialdehyde content and decreased the soluble protein content, total antioxidant and superoxide dismutase activity, and significantly inhibited three photosynthesis-related genes (psaB, psbD, and rbcL). The results demonstrated the algicidal effect of neo-przewaquinone A on M. aeruginosa and provided the possible inhibition mechanisms.
authors.affiliation: Northwest A&F University, Xinong Road 22nd, Yangling, Shaanxi 712100, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Zhang
authors.forename: Yang-Lei
authors.initials: YL
authors.lastname: Yi
authors.forename: Kai
authors.initials: K
authors.lastname: Hao
authors.forename: Guang-Lu
authors.initials: GL
authors.lastname: Liu
authors.forename: Gao-Xue
authors.initials: GX
authors.lastname: Wang
mesh_terms: D001688:Biological Products / Q000633:toxicity*; D004202:Disinfectants / Q000633:toxicity*; D005068:Eutrophication; D006576:Heterocyclic Compounds, 4 or More Rings / Q000633:toxicity*; D046931:Microcystis / Q000187:drug effects*; D010788:Photosynthesis / Q000187:drug effects; D011809:Quinones / Q000633:toxicity*; D027561:Salvia miltiorrhiza / Q000737:chemistry*
chemical_list: D001688:Biological Products; D004202:Disinfectants; D006576:Heterocyclic Compounds, 4 or More Rings; D011809:Quinones; C484875:neoprzewaquinone A
keywords: Algicidal; Cell morphology; Neo-przewaquinone A; Oxidative stress; Photosynthesis-related genes; Salvia miltiorrhiza Bung