title: Validation of a new HPLC-UV method for determination of the antibiotic linezolid in human plasma and in bronchoalveolar lavage.
abstract: A rapid and selective HPLC-UV method was developed for the quantification of linezolid (LNZ) in human plasma and bronchoalveolar lavage (BAL) at the concentrations associated with therapy. Plasma samples were extracted by solid-phase extraction followed by evaporation to dryness and reconstitution in mobile phase solution. The chromatographic separation was carried out on a C18 column with an isocratic mobile phase consisting of dihydrogen phosphate buffer 50 mm (pH 3.5) and acetonitrile (60:40 v/v). The detection was performed using a photodiode array. Under these conditions, a single chromatographic run could be completed within 12 min. The method was validated by estimating the precision and the accuracy for inter- and intra-day analysis in the concentration range of 25-25600 ng/mL. The method was linear over the investigated range with all the correlation coefficients R > 0.999. The intra- and inter-day precision was within 8.90% and the accuracy ranged from -4.76 to +5.20%. This rapid and sensitive method was fully validated and could be applied to pharmacokinetic study for the determination of LNZ levels in human plasma and BAL samples.
authors.affiliation: Clinical Pharmacology Unit, 'Agostino Gemelli' Academic Hospital, Catholic University Medical School, Rome, Italy.
authors.forename: Serena
authors.initials: S
authors.lastname: Fortuna
authors.forename: Gennaro
authors.initials: G
authors.lastname: De Pascale
authors.forename: Enzo
authors.initials: E
authors.lastname: Ragazzoni
authors.forename: Massimo
authors.initials: M
authors.lastname: Antonelli
authors.forename: Pierluigi
authors.initials: P
authors.lastname: Navarra
mesh_terms: D000081:Acetamides / Q000032:analysis* / Q000097:blood*; D000890:Anti-Infective Agents / Q000032:analysis* / Q000097:blood*; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000191:economics / Q000379:methods*; D016903:Drug Monitoring / Q000191:economics / Q000379:methods; D006801:Humans; D057230:Limit of Detection; D000069349:Linezolid; D023303:Oxazolidinones / Q000032:analysis* / Q000097:blood*; D052616:Solid Phase Extraction / Q000191:economics / Q000379:methods; D013056:Spectrophotometry, Ultraviolet; D013997:Time Factors
chemical_list: D000081:Acetamides; D000890:Anti-Infective Agents; D023303:Oxazolidinones; D000069349:Linezolid
keywords: BAL; HPLC-UV; linezolid; solid-phase extraction; therapeutic drug monitoring