title: Resistin levels in gingival crevicular fluid of patients with chronic periodontitis and type 2 diabetes mellitus.
abstract: BACKGROUND
  Resistin is associated with local and systemic inflammatory conditions with a direct correlation with type 2 diabetes mellitus (T2DM). The aim of this clinico-biochemical study is to estimate and compare the levels of resistin in the gingival crevicular fluid (GCF) in health, chronic periodontitis (CP), and T2DM.
  
  
  METHODS
  Sixty patients (aged >35 years) who participated in this study were divided into four groups of 15 patients each: healthy individuals (group 1), patients with CP (group 2), patients with T2DM (group 3), and patients with T2DM and CP (group 4). The parameters assessed included plaque index (PI), gingival index (GI), probing depth (PD), periodontal index, body mass index, random blood sugar (RBS), and glycated hemoglobin (HbA1c). GCF (4 μL) was collected and analyzed for resistin levels using an enzyme-linked immunosorbent assay.
  
  
  RESULTS
  Resistin was detected in the GCF of all patients. A significant difference was observed in GCF resistin concentrations from group 1 versus group 2 (P = 0.0093), group 3 (P = 0.0341), and group 4 (P = 0.0002); in group 2 versus group 4 (P = 0.0032); and in group 3 versus group 4 (P = 0.0008). When all the samples were analyzed together, GCF resistin levels positively correlated with GI, PD, PI, RBS, and HbA1c and were predictable with PD and HbA1c.
  
  
  CONCLUSIONS
  Resistin levels are increased in CP and T2DM. Hence, GCF resistin levels may be considered as a potential inflammatory marker for periodontitis with T2DM.
authors.affiliation: Department of Periodontics, Sri Dharmasthala Manjunatheshwara College of Dental Sciences & Hospital.
authors.forename: Neeraja H
authors.initials: NH
authors.lastname: Gokhale
authors.forename: Anirudh B
authors.initials: AB
authors.lastname: Acharya
authors.forename: Vidya S
authors.initials: VS
authors.lastname: Patil
authors.forename: Dheeraj J
authors.initials: DJ
authors.lastname: Trivedi
authors.forename: Swati
authors.initials: S
authors.lastname: Setty
authors.forename: Srinath L
authors.initials: SL
authors.lastname: Thakur
mesh_terms: D000328:Adult; D016301:Alveolar Bone Loss / Q000145:classification / Q000378:metabolism; D015415:Biomarkers / Q000032:analysis; D001786:Blood Glucose / Q000032:analysis; D015992:Body Mass Index; D055113:Chronic Periodontitis / Q000378:metabolism*; D003774:Dental Plaque Index; D003924:Diabetes Mellitus, Type 2 / Q000378:metabolism*; D005260:Female; D005883:Gingival Crevicular Fluid / Q000737:chemistry*; D006442:Glycated Hemoglobin / Q000032:analysis; D006801:Humans; D018836:Inflammation Mediators / Q000032:analysis; D008297:Male; D008875:Middle Aged; D010512:Periodontal Index; D010514:Periodontal Pocket / Q000145:classification / Q000378:metabolism; D010519:Periodontium / Q000378:metabolism; D052243:Resistin / Q000032:analysis*
chemical_list: D015415:Biomarkers; D001786:Blood Glucose; D006442:Glycated Hemoglobin; D018836:Inflammation Mediators; D052243:Resistin; C499624:RETN protein, human