title: Vitamin B6 in plasma - sample stability and the reference limits.
abstract: Quantitatively, the most important B6 vitamer in plasma is pyridoxal-5'-phosphate (p-PLP). The prerequisite for the use of p-PLP measurements in patients with poor nutritional status is an appropriate reference interval, together with knowledge of the stability of vitamin B6 in plasma samples. We used blood samples from healthy blood donors to derive the reference limits for p-PLP, and to examine its stability for 24 hours at room temperature and at 4-8°C. P-PLP was measured using high performance liquid chromatography (HPLC). The reference interval in adults was 23-223 nmol/L. P-PLP was stable for 24 h at room temperature and at 4-8°C, allowing time for normal specimen transport.
authors.affiliation: Department of Medical Biochemistry, St. Olav's Hospital, Trondheim University Hospital , Trondheim.
authors.forename: Kristine K
authors.initials: KK
authors.lastname: Panton
authors.forename: Per G
authors.initials: PG
authors.lastname: Farup
authors.forename: Erling
authors.initials: E
authors.lastname: Sagen
authors.forename: Unni F
authors.initials: UF
authors.lastname: Sirum
authors.forename: Arne
authors.initials: A
authors.lastname: Asberg
mesh_terms: D000328:Adult; D000368:Aged; D001774:Blood Chemical Analysis; D001793:Blood Preservation; D015925:Cryopreservation; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D011732:Pyridoxal Phosphate / Q000097:blood*; D012016:Reference Values; D025101:Vitamin B 6 / Q000097:blood
chemical_list: D011732:Pyridoxal Phosphate; D025101:Vitamin B 6