title: A superabsorbent polymer-containing wound dressing efficiently sequesters MMPs and inhibits collagenase activity in vitro.
abstract: Superabsorbent polymer (SAP)-containing wound dressings present a valuable and unique category of wound management products. An in vitro approach was used to assess the effects of a new SAP dressing in treatment of non-healing wounds. It was shown that the SAP dressing possesses a significant binding capacity for MMP-2 and MMP-9 in vitro (P\0.001). The inclusion of the bound proteases was so strong that no MMP-2 and only marginal amounts of MMP-9 were released from the dressing samples in a subsequent elution step. In addition, the SAP dressing was able to take up collagenase and reduce its activity in vitro. However, collagenase was not completely inactivated upon binding and enzyme-mediated substrate turnover could be observed at the dressings. In conclusion, in vitro data confirm the positive effect of the SAP wound dressing observed in vivo. The findings suggest that it should be specifically useful for highly exuding wounds with an elevated proteolytic activity that needs to be reduced to support healing.
authors.forename: Cornelia
authors.initials: C
authors.lastname: Wiegand
authors.forename: Uta-Christina
authors.initials: UC
authors.lastname: Hipler
mesh_terms: D000042:Absorption; D001458:Bandages; D017364:Collagenases / Q000737:chemistry*; D004797:Enzyme-Linked Immunosorbent Assay; D005109:Extracellular Matrix / Q000378:metabolism; D006801:Humans; D008422:Materials Testing; D020778:Matrix Metalloproteinase 2 / Q000737:chemistry*; D020780:Matrix Metalloproteinase 9 / Q000737:chemistry*; D061965:Matrix Metalloproteinase Inhibitors / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D011485:Protein Binding; D013997:Time Factors; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D061965:Matrix Metalloproteinase Inhibitors; D011108:Polymers; D017364:Collagenases; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9