title: Beta-arrestin2 is involved in the increase of distal colonic contraction in diabetic rats.
abstract: Colonic dysmotility occurs in diabetes and the patients exhibit diarrhea or constipation. The pathogenetic mechanisms underlying colonic dysmotility in diabetic patients remain poorly understood. The effects of β-arrestin2 on colonic contraction in diabetic rats were investigated for the first time. Male SD rats were treated with a single intraperitoneally injected dose of streptozotocin, and those displaying sustained high blood glucose were selected as diabetes mellitus models. Longitudinal muscle strips of the distal colon were prepared to monitor contraction of the colon in vitro. Expression of β-arrestin2 was investigated by Western blot analysis. Anti-β-arrestin2 antibody had no direct effect on the contraction of distal colonic strips in both normal and diabetic rats. Carbachol-induced contractions of distal colonic strips were higher in diabetic rats than in normal rats. Anti-β-arrestin2 antibody partly blocked carbachol-induced increases of distal colonic strips in diabetic rats. The expression level of β-arrestin2 protein in the colon was higher in diabetic rats than in normal rats. These results suggest that β-arrestin2 is involved in the increase of distal colonic contraction in diabetic rats.
authors.affiliation: Department of Physiology, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai 200092, PR China. Electronic address: xiedping@tongji.edu.cn.
authors.forename: Dong-Ping
authors.initials: DP
authors.lastname: Xie
authors.forename: Sen
authors.initials: S
authors.lastname: Li
authors.forename: Liang
authors.initials: L
authors.lastname: Li
authors.forename: Xin-Wen
authors.initials: XW
authors.lastname: Chang
authors.forename: Tao-Fang
authors.initials: TF
authors.lastname: Xi
authors.forename: Xiao
authors.initials: X
authors.lastname: Yang
authors.forename: Zhi
authors.initials: Z
authors.lastname: Jin
authors.forename: Ying
authors.initials: Y
authors.lastname: Zeng
mesh_terms: D000818:Animals; D019390:Arrestins / Q000494:pharmacology / Q000502:physiology*; D002217:Carbachol / Q000494:pharmacology; D018679:Cholinergic Agonists / Q000494:pharmacology; D003106:Colon / Q000187:drug effects / Q000378:metabolism / Q000503:physiopathology*; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism / Q000503:physiopathology*; D066298:In Vitro Techniques; D008297:Male; D009119:Muscle Contraction; D009130:Muscle, Smooth / Q000187:drug effects / Q000378:metabolism / Q000503:physiopathology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D000071557:beta-Arrestins
chemical_list: D019390:Arrestins; D018679:Cholinergic Agonists; D000071557:beta-Arrestins; D002217:Carbachol
keywords: Colonic motility; Diabetes mellitus; β-Arrestin2