title: [IncP-7 plasmids' classification based on structural diversity of their basic replicons].
abstract: The structural diversity of basic replicons and repB gene of the IncP-7 plasmids' collection was firstly assessed on the basis of PCR, restriction analysis and partial sequencing. It has been revealed that DNA fragment containing gene for UvrD-like helicase RepB is a part of all known P-7 replicons, but often serves as hot place for diverse IS-elements invasion. The first system of P-7 plasmids' classification has been worked out on the basis of determined repA-oriV-par WABC nucleotide divergency. Most degradation plasmids established to be belonging to large beta-subgroup, streptomycin resistance plasmid Rms148 (IncP-7 archetype)--to alpha-subgroup, carbazole degradation plasmid pCAR1 and NAH/SAL-plasmids from pY-line (Yamal oil deposits)--to gamma-subgroup and CAP-plasmid pBS270 with potentially reduced P-7 replicon--to delta-subgroup. It has been observed that the type of IncP-7 basic replicon molecular organization does not correlate with fixed phenotypic character in most cases, that is plasmids encoding different phenotypic markers could be members of the same P-7 subgroup.
authors.forename: O V
authors.initials: OV
authors.lastname: Volkova
authors.forename: A V
authors.initials: AV
authors.lastname: Panov
authors.forename: I A
authors.initials: IA
authors.lastname: Kosheleva
authors.forename: A M
authors.initials: AM
authors.lastname: Boronin
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics; D001673:Biodegradation, Environmental; D004251:DNA Transposable Elements / Q000235:genetics; D014644:Genetic Variation; D009894:Opportunistic Infections / Q000235:genetics / Q000382:microbiology; D010957:Plasmids / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D011110:Polymorphism, Genetic; D011549:Pseudomonas / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity; D018741:Replication Origin / Q000235:genetics; D012093:Replicon / Q000235:genetics*; D012988:Soil Microbiology
chemical_list: D001426:Bacterial Proteins; D004251:DNA Transposable Elements; C048817:RepB protein, Bacteria