title: Role of Bordetella pertussis RseA in the cell envelope stress response and adenylate cyclase toxin release.
abstract: Bordetella pertussis is the bacterial agent of the human disease such as whooping cough. In many bacteria, the extracellular function sigma factor σEis central to the response to envelope stress, and its activity is negatively controlled by the RseA anti-sigma factor. In this study, the role of RseA in B. pertussis envelope stress responses was investigated. Compared with the wild-type strain, an rseA mutant showed elevated resistance to envelope stress and enhanced growth at 25 °C. rpoH and other predicted σEtarget genes demonstrated increased transcription in the rseA mutant compared with the wild-type parent. Transcription of those genes was also increased in wild-type B. pertussis and Escherichia coli under envelope stress, whereas no stress-induced increase in transcription was observed in the rseA mutant. rseA inactivation was also associated with altered levels of certain proteins in culture supernatant fluids, which showed increased adenylate cyclase toxin (CyaA) levels. The increased CyaA in the mutant was correlated with an apparent increased stability of the extracellular toxin and increased production of CyaA-containing outer membrane vesicles. Consistent with this, compared with the wild-type strain, rseA mutant cells produced increased numbers of large surface-associated vesicles.
authors.affiliation: Department of Infectious Diseases, Kyorin University School of Medicine, Mitaka, Tokyo, Japan thanawa@ks.kyorin-u.ac.jp.
authors.forename: Tomoko
authors.initials: T
authors.lastname: Hanawa
authors.affiliation: Department of Infectious Diseases, Kyorin University School of Medicine, Mitaka, Tokyo, Japan.
authors.forename: Hideo
authors.initials: H
authors.lastname: Yonezawa
authors.affiliation: Department of Anatomy, Kyorin University School of Medicine, Mitaka, Tokyo, Japan.
authors.forename: Hayato
authors.initials: H
authors.lastname: Kawakami
authors.affiliation: Department of Infectious Diseases, Kyorin University School of Medicine, Mitaka, Tokyo, Japan.
authors.forename: Shigeru
authors.initials: S
authors.lastname: Kamiya
authors.affiliation: Department of Microbiology, University of Minnesota Medical School, Minneapolis, MN, USA.
authors.forename: Sandra K
authors.initials: SK
authors.lastname: Armstrong
mesh_terms: D037361:Adenylate Cyclase Toxin / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D001886:Bordetella pertussis / Q000235:genetics / Q000254:growth & development / Q000502:physiology*; D004926:Escherichia coli / Q000235:genetics / Q000502:physiology; D017353:Gene Deletion; D015964:Gene Expression Regulation, Bacterial; D013312:Stress, Physiological; D013696:Temperature
chemical_list: D037361:Adenylate Cyclase Toxin; D001426:Bacterial Proteins; C000617691:RseA protein, Bordetella pertussis
keywords: Bordetella pertussis; RpoE; RseA; adenylate cyclase; envelope stress; σE