title: Differential proteomics of Aedes albopictus salivary gland, midgut and C6/36 cell induced by dengue virus infection.
abstract: The interaction between dengue virus (DENV) and vector mosquitoes are still poorly understood at present. In this study, 2-D DIGE combined with MS was used to analyze the differential proteomes of Aedes albopictus salivary gland, midgut and C6/36 cells induced by DENV-2. Our results indicated that the virus infection regulated several functional classes of proteins. Among them, 26 were successfully analyzed by real-time RT-PCR. The mRNA levels of 15 were the highest in salivary gland, 2 in midgut and none in C6/36 cells, however, 18 were the least in fat body compared to other organs. Interestingly, the changes of differential proteins mRNA were the most obvious in fat body post-infection. Chaperone, cytoskeleton and energy metabolism enzyme were the most down- or up- regulated proteins after DENV-2 infection. The abundant expression of these proteins in salivary gland may relate to its high susceptibility.
authors.affiliation: Department of Parasitology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510080, People's Republic of China.
authors.forename: Meichun
authors.initials: M
authors.lastname: Zhang
authors.forename: Xiaoying
authors.initials: X
authors.lastname: Zheng
authors.forename: Yu
authors.initials: Y
authors.lastname: Wu
authors.forename: Ming
authors.initials: M
authors.lastname: Gan
authors.forename: Ai
authors.initials: A
authors.lastname: He
authors.forename: Zhuoya
authors.initials: Z
authors.lastname: Li
authors.forename: Dongjing
authors.initials: D
authors.lastname: Zhang
authors.forename: Xiansheng
authors.initials: X
authors.lastname: Wu
authors.forename: Ximei
authors.initials: X
authors.lastname: Zhan
mesh_terms: D000330:Aedes / Q000737:chemistry*; D000818:Animals; D002460:Cell Line; D003716:Dengue Virus / Q000472:pathogenicity*; D015180:Electrophoresis, Gel, Two-Dimensional; D041981:Gastrointestinal Tract / Q000737:chemistry; D020869:Gene Expression Profiling; D054884:Host-Pathogen Interactions; D013058:Mass Spectrometry; D020543:Proteome / Q000032:analysis*; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D012469:Salivary Glands / Q000737:chemistry
chemical_list: D020543:Proteome; D012333:RNA, Messenger
keywords: Aedes albopictus; Dengue virus; Differential proteomics