title: DaptomycinIn VitroActivity against Methicillin-Resistant Staphylococcus aureus Is Enhanced by d-Cycloserine in a Mechanism Associated with a Decrease in Cell Surface Charge.
abstract: The killing activity of daptomycin against an isogenic pair of daptomycin-susceptible and daptomycin-nonsusceptible (DNS) methicillin-resistantStaphylococcus aureus(MRSA) strains was enhanced by the addition of certain cell wall agents at 1× MIC. However, when high inocula of the DNS strain were used, no significant killing was observed in our experiments. Cytochromecbinding assays revealed d-cycloserine as the only agent associated with a reduction in the cell surface charge for both strains at the concentrations used.
authors.affiliation: Beth Israel Deaconess Medical Center ogasch@bellvitgehospital.cat.|Harvard Medical School, Boston, Massachusetts, USA.|Hospital Universitari de Bellvitge, Barcelona, Spain.
authors.forename: O
authors.initials: O
authors.lastname: Gasch
authors.affiliation: Beth Israel Deaconess Medical Center.|Harvard Medical School, Boston, Massachusetts, USA.
authors.forename: S K
authors.initials: SK
authors.lastname: Pillai
authors.affiliation: Beth Israel Deaconess Medical Center.
authors.forename: J
authors.initials: J
authors.lastname: Dakos
authors.affiliation: Beth Israel Deaconess Medical Center.|Harvard Medical School, Boston, Massachusetts, USA.|Graduate School of Medicine, University of Wollongong, New South Wales, Australia.
authors.forename: S
authors.initials: S
authors.lastname: Miyakis
authors.affiliation: Beth Israel Deaconess Medical Center.|Harvard Medical School, Boston, Massachusetts, USA.
authors.forename: R C
authors.initials: RC
authors.lastname: Moellering
authors.affiliation: Beth Israel Deaconess Medical Center.|Harvard Medical School, Boston, Massachusetts, USA.
authors.forename: G M
authors.initials: GM
authors.lastname: Eliopoulos