title: MT1-MMP regulates MMP-2 expression and angiogenesis-related functions in human umbilical vein endothelial cells.
abstract: Membrane type 1 (MT1)-MMP is a member of matrix metalloproteinases (MMPs) that regulates extracellular matrix remodeling. In addition, MT1-MMP also serves as a multi-functional protein. However, the functional role of MT1-MMP in human endothelial cells remains unclear. In this study we use real-time PCR and Western blotting to demonstrate for the first time that MMP-2 expression is regulated by MT1-MMP in human endothelial cells. Moreover, MMP-2 activity is also modulated by MT1-MMP. In addition we found that endothelial cells, ECM adhesion and human endothelial cell tube formation, which are known to be regulated by MMP-2, are blocked by MT1-MMP siRNA. These results suggest that MT1-MMP plays an important role in regulating angiogenesis in human endothelial cells.
authors.affiliation: Department of Life Science, National Taiwan University, Taipei, Taiwan, ROC.
authors.forename: Hsinyu
authors.initials: H
authors.lastname: Lee
authors.forename: Kai-Wei
authors.initials: KW
authors.lastname: Chang
authors.forename: Hsi-Yuan
authors.initials: HY
authors.lastname: Yang
authors.forename: Po-Wei
authors.initials: PW
authors.lastname: Lin
authors.forename: Shee-Uan
authors.initials: SU
authors.lastname: Chen
authors.forename: Yuan-Li
authors.initials: YL
authors.lastname: Huang
mesh_terms: D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology / Q000201:enzymology*; D006801:Humans; D053511:Matrix Metalloproteinase 14 / Q000235:genetics / Q000502:physiology*; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014471:Umbilical Veins / Q000166:cytology / Q000201:enzymology*
chemical_list: D020778:Matrix Metalloproteinase 2; D053511:Matrix Metalloproteinase 14
keywords: Angiogenesis; HUVECs; MMP-2; MT1-MMP