title: Porous hyaluronic acid/sodium alginate composite scaffolds for human adipose-derived stem cells delivery.
abstract: The aim of this study is to evaluate the feasibility of hyaluronic acid/sodium alginate (HA/SA) scaffold-based interpenetrating polymeric network (IPN) for the proliferation and chondrogenic differentiation of the human adipose-derived stem cells (hADSCs). The hADSCs cultured in HA/SA IPN scaffold exhibited enhanced cell adhesion and proliferation compared to the HA scaffold. Superior chondrogenic differentiation of hADSCs in HA/SA IPN scaffold, compared to HA-based scaffold, was confirmed by measuring expression levels of chondrogenic markers. These results suggested that HA/SA IPN scaffold could provide a desirable environment for the cell adhesion, proliferation and chondrogenic differentiation of hADSCs.
authors.affiliation: College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University, Seoul 151-742, Republic of Korea.
authors.forename: Yun-Jeong
authors.initials: YJ
authors.lastname: Son
authors.forename: In-Soo
authors.initials: IS
authors.lastname: Yoon
authors.forename: Jong-Hyuk
authors.initials: JH
authors.lastname: Sung
authors.forename: Hyun-Jong
authors.initials: HJ
authors.lastname: Cho
authors.forename: Suk-Jae
authors.initials: SJ
authors.lastname: Chung
authors.forename: Chang-Koo
authors.initials: CK
authors.lastname: Shim
authors.forename: Dae-Duk
authors.initials: DD
authors.lastname: Kim
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000464:Alginates / Q000737:chemistry*; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D049109:Cell Proliferation; D020219:Chondrogenesis; D024043:Collagen Type II / Q000378:metabolism; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D006801:Humans; D006820:Hyaluronic Acid / Q000737:chemistry*; D007150:Immunohistochemistry; D016062:Porosity; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism / Q000648:ultrastructure; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D000464:Alginates; D024043:Collagen Type II; D006603:Hexuronic Acids; D020723:Glucuronic Acid; D006820:Hyaluronic Acid
keywords: Chondrogenic differentiation; Human adipose-derived stem cells; Hyaluronic acid; Interpenetrating polymeric network; Sodium alginate