title: Targeting cancer cell-specific RNA interference by siRNA delivery using a complex carrier of affibody-displaying bio-nanocapsules and liposomes.
abstract: BACKGROUND
  Small interfering RNA (siRNA) has attracted attention in the field of nucleic acid medicine as a RNA interference (RNAi) application that leads to gene silencing due to specific messenger RNA (mRNA) destruction. However, since siRNA is unstable in blood and unable to cross the cell membrane, encapsulation of siRNA into a carrier is required.
  
  
  RESULTS
  In this study, we used a carrier that combined ZHER2-displaying bio-nanocapsule (derived from hepatitis B virus surface antigen) and liposomes in a complex in order to investigate the feasibility of effective and target-cell-specific RNAi applications. As a result, by observing RNAi only in HER2-expressing breast cancer cells, using our proposed methodology, we successfully demonstrated target-cell-specific delivery and effective function expression of siRNA.
  
  
  CONCLUSIONS
  These findings show that, in the field of nucleic acid medicine, ZHER2-BNC/LP can be a useful carrier for siRNA delivery, and could also become a useful tool for gene silencing and to accomplish protein knock-down.
authors.affiliation: Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodai, Nada, Kobe 657-8501, Japan.
authors.forename: Yuya
authors.initials: Y
authors.lastname: Nishimura
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Mieda
authors.forename: Jun
authors.initials: J
authors.lastname: Ishii
authors.forename: Chiaki
authors.initials: C
authors.lastname: Ogino
authors.forename: Toshinobu
authors.initials: T
authors.lastname: Fujiwara
authors.forename: Akihiko
authors.initials: A
authors.lastname: Kondo
mesh_terms: D045744:Cell Line, Tumor; D002470:Cell Survival; D004591:Electrophoresis, Polyacrylamide Gel; D005453:Fluorescence; D018014:Gene Transfer Techniques; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006801:Humans; D008027:Light; D008081:Liposomes / Q000737:chemistry*; D053769:Nanocapsules / Q000737:chemistry*; D009369:Neoplasms / Q000378:metabolism*; D010316:Particle Size; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000378:metabolism*; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000378:metabolism*; D015203:Reproducibility of Results; D012542:Scattering, Radiation; D055672:Static Electricity
chemical_list: D049452:Green Fluorescent Proteins; D008081:Liposomes; D053769:Nanocapsules; D034741:RNA, Small Interfering; D018719:Erb-b2 Receptor Tyrosine Kinases; D011993:Recombinant Fusion Proteins