title: Enhancement of skin radical scavenging activity and stratum corneum lipids after the application of a hyperforin-rich cream.
abstract: Hyperforin is well-known for its anti-inflammatory, anti-tumor, anti-bacterial, and antioxidant properties. The application of a hyperforin-rich verum cream could strengthen the skin barrier function by reducing radical formation and stabilizing stratum corneum lipids. Here, it was investigated whether topical treatment with a hyperforin-rich cream increases the radical protection of the skin during VIS/NIR irradiation. Skin lipid profile was investigated applying HPTLC on skin lipid extracts. Furthermore, the absorption- and scattering coefficients, which influence radical formation, were determined. 11 volunteers were included in this study. After a single cream application, VIS/NIR-induced radical formation could be completely inhibited by both verum and placebo showing an immediate protection. After an application period of 4weeks, radical formation could be significantly reduced by 45% following placebo application and 78% after verum application showing a long-term protection. Furthermore, the skin lipids in both verum and placebo groups increased directly after a single cream application but only significantly for ceramide [AP], [NP1], and squalene. After long-term cream application, concentration of cholesterol and the ceramides increased, but no significance was observed. These results indicate that regular application of the hyperforin-rich cream can reduce radical formation and can stabilize skin lipids, which are responsible for the barrier function.
authors.affiliation: Center of Experimental and Applied Cutaneous Physiology, Department of Dermatology and Allergy, Charité - Universitätsmedizin Berlin, Germany. Electronic address: stefan.haag@charite.de.
authors.forename: S F
authors.initials: SF
authors.lastname: Haag
authors.affiliation: Institute of Food Chemistry, Hamburg School of Food Science, University of Hamburg, Germany. Electronic address: Kathrin.Tscherch@chemie.uni-hamburg.de.
authors.forename: K
authors.initials: K
authors.lastname: Tscherch
authors.affiliation: Center of Experimental and Applied Cutaneous Physiology, Department of Dermatology and Allergy, Charité - Universitätsmedizin Berlin, Germany. Electronic address: Sophia.Arndt@charite.de.
authors.forename: S
authors.initials: S
authors.lastname: Arndt
authors.affiliation: Klosterfrau Berlin GmbH, Berlin, Germany. Electronic address: Anke.Kleemann@cassella-med.de.
authors.forename: A
authors.initials: A
authors.lastname: Kleemann
authors.affiliation: Laser- und Medizin-Technologie GmbH, Berlin, Germany. Electronic address: i.gersonde@lmtb.de.
authors.forename: I
authors.initials: I
authors.lastname: Gersonde
authors.affiliation: Center of Experimental and Applied Cutaneous Physiology, Department of Dermatology and Allergy, Charité - Universitätsmedizin Berlin, Germany. Electronic address: Juergen.Lademann@charite.de.
authors.forename: J
authors.initials: J
authors.lastname: Lademann
authors.affiliation: Institute of Food Chemistry, Hamburg School of Food Science, University of Hamburg, Germany. Electronic address: rohn@chemie.uni-hamburg.de.
authors.forename: S
authors.initials: S
authors.lastname: Rohn
authors.affiliation: Center of Experimental and Applied Cutaneous Physiology, Department of Dermatology and Allergy, Charité - Universitätsmedizin Berlin, Germany. Electronic address: Martina.Meinke@charite.de.
authors.forename: M C
authors.initials: MC
authors.lastname: Meinke
mesh_terms: D000328:Adult; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage* / Q000378:metabolism; D002518:Ceramides / Q000378:metabolism; D005260:Female; D016166:Free Radical Scavengers / Q000008:administration & dosage* / Q000378:metabolism; D006801:Humans; D008055:Lipids; D008297:Male; D010696:Phloroglucinol / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000378:metabolism; D012867:Skin / Q000187:drug effects* / Q000378:metabolism; D012869:Skin Absorption; D063465:Skin Cream / Q000378:metabolism / Q000627:therapeutic use*; D013729:Terpenes / Q000008:administration & dosage* / Q000378:metabolism; D055815:Young Adult
chemical_list: D000893:Anti-Inflammatory Agents; D002518:Ceramides; D016166:Free Radical Scavengers; D008055:Lipids; D013729:Terpenes; D010696:Phloroglucinol; C001654:hyperforin
keywords: Electron paramagnetic resonance spectroscopy; High performance thin layer chromatography; Hypericum perforatum; Skin lipid profile; St. John’s Wort