title: CSE1L modulates Ras-induced cancer cell invasion: correlation of K-Ras mutation and CSE1L expression in colorectal cancer progression.
abstract: BACKGROUND
  Ras plays an important role in colorectal cancer progression. CSE1L (chromosome segregation 1-like) gene maps to 20q13, a chromosomal region that correlates with colorectal cancer development. We investigated the association of CSE1L with Ras in colorectal cancer progression.
  
  
  METHODS
  The effect of CSE1L on metastasis-stimulating activity of Ras was studied in an animal model with tumor cells expressing CSE1L-specific shRNA and v-H-Ras. CSE1L expression was evaluated by the immunohistochemical analysis of 127 surgically resected colorectal tumors. K-Ras mutations were analyzed by direct sequencing.
  
  
  RESULTS
  CSE1L knockdown reduced Ras-induced metastasis of B16F10 melanoma cells in C57BL/6 mice. v-H-Ras expression altered the cellular trafficking of CSE1L and increased CSE1L secretion. Most colorectal tumors were positive for CSE1L staining (98.4%, 125 of 127). Colorectal tumors with K-Ras mutation or high cytoplasmic CSE1L expression were correlated with T status (depth of tumor penetration; P = .004), stage (P = .004), and lymph node metastasis (P = .019).
  
  
  CONCLUSIONS
  CSE1L may be a target for treating Ras-associated tumors. Analysis of K-Ras mutation and CSE1L expression may provide valuable clinical and pathological information to aid in the determination of treatment options for colorectal cancer.
authors.affiliation: Department of Internal Medicine, School of Medicine, College of Medicine, Taipei Medical University, Hsing-Yi District, Taiwan.
authors.forename: Ming-Chung
authors.initials: MC
authors.lastname: Jiang
authors.forename: Chung-Min
authors.initials: CM
authors.lastname: Yeh
authors.forename: Cheng-Jeng
authors.initials: CJ
authors.lastname: Tai
authors.forename: Hung-Chang
authors.initials: HC
authors.lastname: Chen
authors.forename: Shu-Hui
authors.initials: SH
authors.lastname: Lin
authors.forename: Tzu-Cheng
authors.initials: TC
authors.lastname: Su
authors.forename: Shing-Chuan
authors.initials: SC
authors.lastname: Shen
authors.forename: Woan-Ruoh
authors.initials: WR
authors.lastname: Lee
authors.forename: Ching-Fong
authors.initials: CF
authors.lastname: Liao
authors.forename: Li-Tzu
authors.initials: LT
authors.lastname: Li
authors.forename: Ching-Hsiao
authors.initials: CH
authors.lastname: Lee
authors.forename: Ying-Chun
authors.initials: YC
authors.lastname: Chen
authors.forename: Kun-Tu
authors.initials: KT
authors.lastname: Yeh
authors.forename: Chun-Chao
authors.initials: CC
authors.lastname: Chang
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D014408:Biomarkers, Tumor / Q000235:genetics; D001706:Biopsy; D029563:Cellular Apoptosis Susceptibility Protein / Q000235:genetics*; D003062:Codon; D015179:Colorectal Neoplasms / Q000235:genetics*; D018450:Disease Progression; D005260:Female; D005455:Fluorescent Antibody Technique; D011905:Genes, ras / Q000235:genetics*; D020022:Genetic Predisposition to Disease; D006801:Humans; D015151:Immunoblotting; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008875:Middle Aged; D009154:Mutation; D009362:Neoplasm Metastasis / Q000235:genetics*
chemical_list: D014408:Biomarkers, Tumor; D029563:Cellular Apoptosis Susceptibility Protein; D003062:Codon
keywords: CSE1L; Colorectal cancer; K-Ras; Lymph node; Metastasis