title: Laboratory and human studies to estimate anticaries efficacy of fluoride toothpastes.
abstract: Much more than mechanical biofilm removal, toothbrushing with fluoride toothpastes is an effective way of increasing the availability of fluoride in the oral cavity to reduce demineralization and enhance remineralization of enamel and dentine. These effects of fluoride toothpastes have been estimated by a wide range of laboratory and human studies, which have helped to develop anticaries effective formulations and understand their mechanism of action. These studies have focused on the availability of fluoride in the toothpaste formulations, its bioavailability in saliva and remnants of disturbed biofilm, its reaction with the dental substrate to form loosely bound reservoirs as well as the ultimate reduction of mineral loss and increase in mineral and fluoride content of caries lesions. The specifics of these modes of action and their application in in vitro, in situ and in vivo preclinical tests is presented and discussed.
authors.affiliation: Piracicaba Dental School, University of Campinas, Piracicaba, Brazil.
authors.forename: Livia M A
authors.initials: LM
authors.lastname: Tenuta
authors.forename: Jaime A
authors.initials: JA
authors.lastname: Cury
mesh_terms: D001682:Biological Availability; D002327:Cariostatic Agents / Q000737:chemistry / Q000493:pharmacokinetics / Q000627:therapeutic use*; D002626:Chemistry, Pharmaceutical; D004353:Drug Evaluation, Preclinical; D005459:Fluorides / Q000737:chemistry / Q000493:pharmacokinetics / Q000627:therapeutic use*; D006801:Humans; D017001:Tooth Demineralization / Q000378:metabolism / Q000517:prevention & control; D014089:Tooth Remineralization / Q000379:methods; D014099:Toothbrushing; D014100:Toothpastes / Q000737:chemistry / Q000493:pharmacokinetics / Q000627:therapeutic use*
chemical_list: D002327:Cariostatic Agents; D014100:Toothpastes; D005459:Fluorides