title: Phosphorylation of neuronal nitric oxide synthase at Ser1412 in the dentate gyrus of rat brain after transient forebrain ischemia.
abstract: We previously demonstrated that calmodulin-dependent protein kinase IIα (CaM-KIIα) phosphorylates nNOS at Ser(847) in the hippocampus after forebrain ischemia; this phosphorylation attenuates NOS activity and might contribute to resistance to post-ischemic damage. We also revealed that cyclic AMP-dependent protein kinase (PKA) could phosphorylate nNOS at Ser(1412)in vitro. In this study, we focused on chronological and topographical changes in the phosphorylation of nNOS at Ser(1412) after rat forebrain ischemia. The hippocampus and adjacent cortex were collected at different times, up to 24h, after 15min of forebrain ischemia. NOS was partially purified from crude samples using ADP agarose gel. Neuronal NOS, phosphorylated (p)-nNOS at Ser(1412), PKA, and p-PKA at Thr(197) were studied in the rat hippocampus and cortex using Western blot analysis and immunohistochemistry. Western blot analysis revealed that p-nNOS at Ser(1412) significantly increased between 1 and 6h after reperfusion in the hippocampus, but not in the cortex. PKA was cosedimented with nNOS by ADP agarose gel. Immunohistochemistry revealed that phosphorylation of nNOS at Ser(1412) and PKA at Thr(197) occurred in the subgranular layer of the dentate gyrus. Forebrain ischemia might thereby induce temporary activation of PKA at Thr(197), which then phosphorylates nNOS at Ser(1412) in the subgranular layer of the dentate gyrus.
authors.affiliation: Department of Neurological Surgery, Aichi Medical University, 1-1 Karimata Yazako, Nagakute, Aichi 480-1195, Japan.
authors.forename: Koji
authors.initials: K
authors.lastname: Osuka
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Watanabe
authors.forename: Nobuteru
authors.initials: N
authors.lastname: Usuda
authors.forename: Kimie
authors.initials: K
authors.lastname: Atsuzawa
authors.forename: Masakazu
authors.initials: M
authors.lastname: Takayasu
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002545:Brain Ischemia / Q000378:metabolism* / Q000473:pathology; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism; D018891:Dentate Gyrus / Q000378:metabolism*; D052248:Nitric Oxide Synthase Type I / Q000737:chemistry / Q000378:metabolism*; D010766:Phosphorylation; D016548:Prosencephalon / Q000473:pathology*; D051381:Rats; D012694:Serine / Q000378:metabolism*
chemical_list: D012694:Serine; D052248:Nitric Oxide Synthase Type I; D017868:Cyclic AMP-Dependent Protein Kinases
keywords: Cerebral ischemia; Cyclic AMP-dependent protein kinase; Neuronal nitric oxide synthase; Phosphorylation