title: Human but Not Laboratory Borna Disease Virus Inhibits Proliferation and Induces Apoptosis in Human Oligodendrocytes In Vitro.
abstract: Borna disease virus (BDV) is a neurotropic virus that produces neuropsychiatric dysfunction in a wide range of warm-blooded species. Several studies have associated BDV with human psychiatric illness, but the findings remain controversial. Although oligodendrocytes are a major glial component of brain white matter and play a pivotal role in neuronal cell function, BDV's effects on human oligodendrocytes have not been clarified. Here, the effects of two BDV strains, Hu-H1 (isolated from a bipolar patient) and Strain V (a laboratory strain), on the proliferation and apoptosis of human oligodendrocytes were investigated. Three experimental cell lines were constructed: Hu-H1-infected oligodendroglioma (Hu-H1) cells, Strain V-infected oligodendroglioma (Strain V) cells, and non-infected oligodendroglioma (control) cells. BDV infection was assayed by BDV nucleoprotein (p40) immunofluorescence, cell proliferation was assayed by Cell Counting Kit-8 (CCK8), and cell cycle phases and apoptosis were assayed by flow cytometry. Expressions of the apoptosis-related proteins Bax and Bcl-2 were measured by Western blotting. p40 expression was confirmed in Hu-H1 and Strain V on and after day three post-infection. Strain V cells showed significantly greater cellular proliferation than Hu-H1 cells on and after day three post-infection. In Hu-H1 cells, Bax and Bcl-2 expression were significantly increased and decreased, respectively, on and after day three post-infection. In contrast, in Strain V cells, Bax and Bcl-2 expression were significantly decreased and increased, respectively, on and after day three post-infection. In conclusion, Hu-H1 inhibits cellular proliferation and promotes apoptosis in human oligodendrocytes via Bax upregulation and Bcl-2 downregulation. In contrast, Strain V promotes cellular proliferation and inhibits apoptosis in human oligodendrocytes via Bax downregulation and Bcl-2 upregulation. The effects of the Hu-H1 strain (isolated from a bipolar patient) are opposite from those of Strain V (a laboratory strain), thereby providing a proof of authenticity for both.
authors.affiliation: Department of Pathology, Faculty of Basic Medicine, Chongqing Medical University, Chongqing, China ; Neuroscience Center, Key Laboratory of Neurobiology of Chongqing, Chongqing, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Li
authors.forename: Yang
authors.initials: Y
authors.lastname: Lei
authors.forename: Jing
authors.initials: J
authors.lastname: Deng
authors.forename: Chanjuan
authors.initials: C
authors.lastname: Zhou
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhang
authors.forename: Wenjuan
authors.initials: W
authors.lastname: Li
authors.forename: Hua
authors.initials: H
authors.lastname: Huang
authors.forename: Shigang
authors.initials: S
authors.lastname: Cheng
authors.forename: Hongzhi
authors.initials: H
authors.lastname: Zhang
authors.forename: Liang
authors.initials: L
authors.lastname: Zhang
authors.forename: Rongzhong
authors.initials: R
authors.lastname: Huang
authors.forename: Xia
authors.initials: X
authors.lastname: Liu
authors.forename: Lihua
authors.initials: L
authors.lastname: Ma
authors.forename: Xiao
authors.initials: X
authors.lastname: Wang
authors.forename: Juan
authors.initials: J
authors.lastname: Li
authors.forename: Peng
authors.initials: P
authors.lastname: Xie
mesh_terms: D017209:Apoptosis; D001714:Bipolar Disorder / Q000378:metabolism / Q000473:pathology / Q000821:virology; D001890:Borna Disease / Q000378:metabolism / Q000473:pathology; D001891:Borna disease virus / Q000378:metabolism*; D002460:Cell Line; D049109:Cell Proliferation; D006801:Humans; D009836:Oligodendroglia / Q000378:metabolism* / Q000821:virology; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000096:biosynthesis; D051028:bcl-2-Associated X Protein / Q000096:biosynthesis
chemical_list: C494826:BAX protein, human; C000595707:BCL2 protein, human; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein