title: Response surface optimization of enzyme-assisted extraction polysaccharides from Dictyophora indusiata.
abstract: An enzyme-assisted procedure for the extraction of the water-soluble polysaccharides from the stipe of Dictyophora indusiata was investigated using response surface methodology. The orthogonal array design was employed to optimize the concentration of three kinds of enzyme (cellulase, papain and pectolyase) and the optimal cellulose, papain and pectolyase concentration were 2.0% (wt.% of D. indusiata powder), 2.0% and 1.5%, respectively. And then the effect such as temperature, time and pH was studied based on a three-level three-factor Box-Behnken design. The optimized conditions were as follows: extraction temperature 52.5 °C, extraction time 105 min and pH 5.25. Under these conditions, the experimental yield of polysaccharides was 9.77±0.18%, which was well matched with the predictive yield of 9.87%. As it turned out, enzyme-assisted procedure was an effective method.
authors.affiliation: School of Chemical Engineering and Technology, Tianjin University, Tianjin, PR China.
authors.forename: Songhai
authors.initials: S
authors.lastname: Wu
authors.forename: Guili
authors.initials: G
authors.lastname: Gong
authors.forename: Yanyan
authors.initials: Y
authors.lastname: Wang
authors.forename: Feng
authors.initials: F
authors.lastname: Li
authors.forename: Shaoyi
authors.initials: S
authors.lastname: Jia
authors.forename: Fengxiang
authors.initials: F
authors.lastname: Qin
authors.forename: Haitao
authors.initials: H
authors.lastname: Ren
authors.forename: Yong
authors.initials: Y
authors.lastname: Liu
mesh_terms: D001487:Basidiomycota / Q000737:chemistry*; D004798:Enzymes / Q000737:chemistry*; D062610:Fungal Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification*; D006863:Hydrogen-Ion Concentration; D059625:Liquid-Liquid Extraction / Q000379:methods; D015203:Reproducibility of Results; D013696:Temperature; D013997:Time Factors
chemical_list: D004798:Enzymes; D062610:Fungal Polysaccharides
keywords: Dictyophora indusiata polysaccharides; Enzyme-assisted extraction; Optimization; Response surface methodology