title: Adhesion and Invasion of Breast and Oesophageal Cancer Cells Are Impeded by Anti-LRP/LR-Specific Antibody IgG1-iS18.
abstract: Adhesion and invasion have been identified as the two key components of metastasis. The 37 kDa/67 kDa laminin receptor (LRP/LR) is thought to enhance these two processes thus endorsing the progression of cancer. Here we report on LRP/LR and the metastatic potential of MDA-MB 231 breast and WHCO1 oesophageal cancer cells. Western blot analysis revealed a significant increase in total laminin receptor precursor (LRP) levels of breast and oesophageal cancer cells in comparison to non-invasive MCF-7 breast cancer cells, whereas LRP/LR cell surface levels in both cell lines were not significantly different to those of MCF-7 cells as analysed by flow cytometry. Incubation of breast and oesophageal cancer cells with the anti-LRP/LR specific antibody, IgG1-iS18, resulted in significant reduction in the adhesive potential of WHCO1 and MDA-MB 231 cells by 92% and 16%, respectively. Moreover, invasion was significantly impeded by 98% and 25% for WHCO1 and MDA-MB 231 cells, respectively. Pearson's correlation coefficients proved a positive correlation between total LRP/LR levels and invasive potential as well as between the adhesive and invasive potential of breast and oesophageal cancer cells. Our findings suggest that through interference of the LRP/LR-laminin-1 interaction, anti-LRP/LR specific antibody IgG1-iS18 may act as a possible alternative therapeutic tool for metastatic breast and oesophageal cancer treatment.
authors.affiliation: School of Molecular and Cell Biology, University of the Witwatersrand, Johannesburg, Gauteng, The Republic of South Africa.
authors.forename: Thandokuhle
authors.initials: T
authors.lastname: Khumalo
authors.forename: Uwe
authors.initials: U
authors.lastname: Reusch
authors.forename: Stefan
authors.initials: S
authors.lastname: Knackmuss
authors.forename: Melvyn
authors.initials: M
authors.lastname: Little
authors.forename: Robin B
authors.initials: RB
authors.lastname: Veale
authors.forename: Stefan F T
authors.initials: SF
authors.lastname: Weiss
mesh_terms: D000906:Antibodies / Q000276:immunology*; D001943:Breast Neoplasms / Q000473:pathology*; D002448:Cell Adhesion / Q000276:immunology*; D045744:Cell Line, Tumor; D004938:Esophageal Neoplasms / Q000473:pathology*; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology*; D008297:Male; D009361:Neoplasm Invasiveness / Q000276:immunology*; D017474:Receptors, Laminin / Q000276:immunology*
chemical_list: D000906:Antibodies; D007074:Immunoglobulin G; D017474:Receptors, Laminin