title: Differential gene expressions of the MAPK signaling pathway in enterovirus 71-infected rhabdomyosarcoma cells.
abstract: BACKGROUND
  Mitogen-activated protein kinase (MAPK) signaling pathway plays an important role in response to viral infection. The aim of this study was to explore the function and mechanism of MAPK signaling pathway in enterovirus 71 (EV71) infection of human rhabdomyosarcoma (RD) cells.
  
  
  METHODS
  Apoptosis of RD cells was observed using annexin V-FITC/PI binding assay under a fluorescence microscope. Cellular RNA was extracted and transcribed to cDNA. The expressions of 56 genes of MAPK signaling pathway in EV71-infected RD cells at 8h and 20h after infection were analyzed by PCR array. The levels of IL-2, IL-4, IL-10, and TNF-α in the supernatant of RD cells infected with EV71 at different time points were measured by ELISA.
  
  
  RESULTS
  The viability of RD cells decreased obviously within 48h after EV71 infection. Compared with the control group, EV71 infection resulted in the significantly enhanced releases of IL-2, IL-4, IL-10 and TNF-α from infected RD cells (p<0.05). At 8h after infection, the expressions of c-Jun, c-Fos, IFN-β, MEKK1, MLK3 and NIK genes in EV71-infected RD cells were up-regulated by 2.08-6.12-fold, whereas other 19 genes (e.g. AKT1, AKT2, E2F1, IKK and NF-κB1) exhibited down-regulation. However, at 20h after infection, those MAPK signaling molecules including MEKK1, ASK1, MLK2, MLK3, NIK, MEK1, MEK2, MEK4, MEK7, ERK1, JNK1 and JNK2 were up-regulated. In addition, the expressions of AKT2, ELK1, c-Jun, c-Fos, NF-κB p65, PI3K and STAT1 were also increased.
  
  
  CONCLUSIONS
  EV71 infection induces the differential gene expressions of MAPK signaling pathway such as ERK, JNK and PI3K/AKT in RD cells, which may be associated with the secretions of inflammatory cytokines and host cell apoptosis.
authors.affiliation: Department of Clinical Laboratory, Third Affiliated Hospital of Suzhou University, Changzhou, Jiangsu, China. : shiweifeng67@163.com
authors.forename: Weifeng
authors.initials: W
authors.lastname: Shi
authors.forename: Xueling
authors.initials: X
authors.lastname: Hou
authors.forename: Xiang
authors.initials: X
authors.lastname: Li
authors.forename: Hongjun
authors.initials: H
authors.lastname: Peng
authors.forename: Mei
authors.initials: M
authors.lastname: Shi
authors.forename: Qingbo
authors.initials: Q
authors.lastname: Jiang
authors.forename: Xiping
authors.initials: X
authors.lastname: Liu
authors.forename: Yun
authors.initials: Y
authors.lastname: Ji
authors.forename: Yuhua
authors.initials: Y
authors.lastname: Yao
authors.forename: Caizhen
authors.initials: C
authors.lastname: He
authors.forename: Xiangdong
authors.initials: X
authors.lastname: Lei
mesh_terms: D016207:Cytokines / Q000235:genetics; D029821:Enterovirus A, Human / Q000201:enzymology / Q000235:genetics* / Q000502:physiology; D004797:Enzyme-Linked Immunosorbent Assay; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D020928:Mitogen-Activated Protein Kinases / Q000235:genetics* / Q000502:physiology; D016133:Polymerase Chain Reaction; D012208:Rhabdomyosarcoma / Q000201:enzymology / Q000235:genetics / Q000821:virology*; D015398:Signal Transduction / Q000235:genetics / Q000502:physiology; D013997:Time Factors; D014407:Tumor Cells, Cultured; D015854:Up-Regulation; D014779:Virus Replication
chemical_list: D016207:Cytokines; D020928:Mitogen-Activated Protein Kinases
keywords: Enterovirus 71; MAPK; PCR array; Rhabdomyosarcoma cells