title: Determination of leelamine in mouse plasma by LC-MS/MS and its pharmacokinetics.
abstract: Leelamine may be applicable to treat diabetes and is known to inhibit pyruvate dehydrogenase kinase 4. In this study, we developed and validated a quantification method using liquid chromatography (LC) coupled with tandem mass spectrometry analysis, which was applied to a pharmacokinetic investigation in mouse plasma. Leelamine transition ions in multiple reaction-monitoring modes using positive ionization were observed at m/z 286.4 to m/z 173.2. LC was performed using an ACE 5 C18 column, and a mixture of acetonitrile and water containing 0.1% formic acid was used as the mobile phase at a flow rate of 0.22mL/min. Leelamine and the internal standard (reserpine) had retention times of 4.1 and 3.9min, respectively. Acceptable linearity (r(2)=0.995) was observed over the concentration range of 10-3000ng/mL, with a lower limit of quantification of 10ng/mL in mouse plasma. The intra-day and inter-day accuracy and precision were less than 15%, which was sufficient for quality-control purposes. This method was used to determine leelamine concentrations in mouse plasma and showed that the oral bioavailability of leelamine was 7.6%.
authors.affiliation: College of Pharmacy, Research Institute of Pharmaceutical Sciences, Kyungpook National University, Daegu 702-701, South Korea.
authors.forename: Min
authors.initials: M
authors.lastname: Song
authors.forename: Doohyun
authors.initials: D
authors.lastname: Lee
authors.forename: Taeho
authors.initials: T
authors.lastname: Lee
authors.forename: Sangkyu
authors.initials: S
authors.lastname: Lee
mesh_terms: D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D004224:Diterpenes / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D004224:Diterpenes