title: High sensitivity point-of-care device for direct virus diagnostics.
abstract: Influenza infections are associated with high morbidity and mortality, carry the risk of pandemics, and pose a considerable economic burden worldwide. To improve the management of the illness, it is essential with accurate and fast point-of-care diagnostic tools for use in the field or at the patient's bedside. Conventional diagnostic methods are time consuming, expensive and require specialized laboratory facilities. We present a highly sensitive, highly specific, and low cost platform to test for acute virus infections in less than 15 min, employing influenza A virus (H1N1) as an example of its usability. An all polymer microfluidic system with a functionalized conductive polymer (PEDOT-OH:TsO) microelectrode array was developed and exploited for label free and real time electrochemical detection of intact influenza A virus (H1N1) particles. DNA aptamers with affinity for influenza A virus (H1N1) were linked covalently to the conductive polymer microelectrodes in the microfluidic channel. Based on changes in the impedance when virions were captured by immobilized probes, we could detect clinically relevant concentrations of influenza A virus (H1N1) in saliva. This is a new, stable and very sensitive point-of-care platform for detection and diagnostics of intact virus particles.
authors.affiliation: Technical University of Denmark, Department of Micro- and Nanotechnology, Oersteds Plads 345 East, DK-2800 Kongens Lyngby, Denmark.
authors.forename: Katrine
authors.initials: K
authors.lastname: Kiilerich-Pedersen
authors.forename: Johannes
authors.initials: J
authors.lastname: Daprà
authors.forename: Solène
authors.initials: S
authors.lastname: Cherré
authors.forename: Noemi
authors.initials: N
authors.lastname: Rozlosnik
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry; D015374:Biosensing Techniques / Q000191:economics / Q000379:methods; D019086:Bridged Bicyclo Compounds, Heterocyclic / Q000737:chemistry; D058266:Dielectric Spectroscopy / Q000191:economics / Q000379:methods; D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype / Q000302:isolation & purification*; D007251:Influenza, Human / Q000175:diagnosis*; D046210:Microfluidic Analytical Techniques / Q000191:economics / Q000379:methods; D019095:Point-of-Care Systems / Q000191:economics; D011108:Polymers / Q000737:chemistry; D012680:Sensitivity and Specificity
chemical_list: D052157:Aptamers, Nucleotide; D019086:Bridged Bicyclo Compounds, Heterocyclic; D011108:Polymers; C121383:poly(3,4-ethylene dioxythiophene)
keywords: AFM; Conductive polymer; DNA aptamer; Electrochemical impedance spectroscopy (EIS); Influenza A virus (H1N1); PEDOT-OH:TsO; Point-of-care (POC)