title: Direct regulation of abiotic responses by the Arabidopsis circadian clock component PRR7.
abstract: Up to 30% of the plant transcriptome is circadian clock-regulated in different species; however, we still lack a good understanding of the mechanisms involved in these genome-wide oscillations in gene expression. Here, we show that PSEUDO-RESPONSE REGULATOR 7 (PRR7), a central component of the Arabidopsis clock, is directly involved in the repression of master regulators of plant growth, light signaling and stress responses. The expression levels of most PRR7 target genes peak around dawn, in an antiphasic manner to PRR7 protein levels, and were repressed by PRR7. These findings indicate that PRR7 is important for cyclic gene expression by repressing the transcription of morning-expressed genes. In particular we found an enrichment of the genes involved in abiotic stress responses, and in accordance we observed that PRR7 is involved in the oxidative stress response and the regulation of stomata conductance.
authors.affiliation: Department of Plant Biology, Michigan State University, East Lansing, MI, 48824, USA.
authors.forename: Tiffany
authors.initials: T
authors.lastname: Liu
authors.forename: Jenny
authors.initials: J
authors.lastname: Carlsson
authors.forename: Tomomi
authors.initials: T
authors.lastname: Takeuchi
authors.forename: Linsey
authors.initials: L
authors.lastname: Newton
authors.forename: Eva M
authors.initials: EM
authors.lastname: Farré
mesh_terms: D000040:Abscisic Acid / Q000378:metabolism; D000222:Adaptation, Physiological; D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics* / Q000502:physiology / Q000528:radiation effects; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D001665:Binding Sites; D047369:Chromatin Immunoprecipitation; D057906:Circadian Clocks; D018506:Gene Expression Regulation, Plant; D059014:High-Throughput Nucleotide Sequencing; D007501:Iron / Q000494:pharmacology; D008027:Light; D009154:Mutation; D018384:Oxidative Stress; D010641:Phenotype; D018515:Plant Leaves / Q000187:drug effects / Q000235:genetics / Q000502:physiology / Q000528:radiation effects; D018526:Plant Transpiration / Q000502:physiology; D030821:Plants, Genetically Modified; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D036226:Seedlings / Q000187:drug effects / Q000235:genetics / Q000502:physiology / Q000528:radiation effects; D017422:Sequence Analysis, DNA; D015398:Signal Transduction
chemical_list: D029681:Arabidopsis Proteins; C523308:PRR7 protein, Arabidopsis; D012097:Repressor Proteins; D000040:Abscisic Acid; D007501:Iron
keywords: Arabidopsis thaliana; ChIP-seq; abscisic acid; circadian clock; oxidative stress; pseudo-response regulator; stomata conductance