title: Suppression of inducible nitric oxide synthase expression induced by Toll-like receptor agonists by (E)-1-(2-(2-nitrovinyl)phenyl)pyrrolidine.
abstract: Toll-like receptors (TLRs) recognize many pathogen-associated molecular patterns and induce innate immunity. TLR signaling pathways induce the activation of various transcription factors, such as nuclear factor-κB (NF-κB), leading to the induction of pro-inflammatory gene products, such as inducible nitric oxide synthase (iNOS). Here, we investigated the effect of an (E)-1-(2-(2-nitrovinyl)phenyl)pyrrolidine (NVPP), previously synthesized in our laboratory, on inflammation by modulating NF-κB activation and iNOS expression induced by TLR agonists in murine macrophages. NVPP suppressed NF-κB activation and iNOS expression induced by lipopolysaccharide (TLR4 agonist), polyriboinosinic polyribocytidylic acid (TLR3 agonist), and macrophage-activating lipopeptide 2kDa (TLR2 and TLR6 agonist). All the results suggest that NVPP is suitable for development as a new anti-inflammatory drug.
authors.affiliation: Department of Biomedical Laboratory Science, Soonchunhyang University, Chungnam, Asan, Republic of Korea.
authors.forename: Sang-Hoon
authors.initials: SH
authors.lastname: Eom
authors.forename: Gyo-Jeong
authors.initials: GJ
authors.lastname: Gu
authors.forename: Chang Won
authors.initials: CW
authors.lastname: Suh
authors.forename: Kwang Oh
authors.initials: KO
authors.lastname: Koh
authors.forename: Dae Young
authors.initials: DY
authors.lastname: Kim
authors.forename: Yong-Bin
authors.initials: YB
authors.lastname: Eom
authors.forename: Hyung-Sun
authors.initials: HS
authors.lastname: Youn
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000138:chemical synthesis / Q000494:pharmacology*; D002460:Cell Line; D005786:Gene Expression Regulation / Q000187:drug effects; D055666:Lipopeptides / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000494:pharmacology; D051379:Mice; D009000:Monocytes / Q000187:drug effects* / Q000276:immunology; D016328:NF-kappa B / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II / Q000037:antagonists & inhibitors*; D011070:Poly I-C / Q000494:pharmacology; D011759:Pyrrolidines / Q000138:chemical synthesis / Q000494:pharmacology*; D013343:Styrenes / Q000138:chemical synthesis / Q000494:pharmacology*; D051193:Toll-Like Receptors / Q000819:agonists / Q000037:antagonists & inhibitors*; D015533:Transcriptional Activation / Q000187:drug effects
chemical_list: C587911:1-(2-(2-nitrovinyl)phenyl)pyrrolidine; D000894:Anti-Inflammatory Agents, Non-Steroidal; D055666:Lipopeptides; D008070:Lipopolysaccharides; D016328:NF-kappa B; D011759:Pyrrolidines; D013343:Styrenes; D051193:Toll-Like Receptors; D009569:Nitric Oxide; C106335:macrophage stimulatory lipopeptide 2; D052247:Nitric Oxide Synthase Type II; D011070:Poly I-C
keywords: (E)-1-(2-(2-nitrovinyl)phenyl)pyrrolidine; Inducible nitric oxide synthase; Inflammation; Nuclear factor-κB; Toll-like receptor