title: Involvement of abscisic acid in regulating antioxidative defense systems and IAA-oxidase activity and improving adventitious rooting in mung bean [Vigna radiata (L.) Wilczek] seedlings under cadmium stress.
abstract: In vitro experiments were conducted to investigate the effects of abscisic acid (ABA) and Cd on antioxidative defense systems and indole-3-acetic acid (IAA) oxidase during adventitious rooting in mung bean [Vigna radiata (L.) Wilczek] seedlings. The exogenous ABA significantly enhanced the number and fresh weight of the adventitious roots. CdCl2 strongly inhibited adventitious rooting. Pretreatment with 10 μM ABA clearly alleviated the inhibitory effect of Cd on rooting. ABA significantly reduced superoxide dismutase (SOD), ascorbate peroxidase (APX), peroxidase (POD), and catalase (CAT) activities, as well as the levels of glutathione (GSH) and ascorbic acid (ASA) during adventitious rooting. ABA strongly increased IAA-oxidase activity during the induction (0-12 h) and expression (after 48 h) phases and increased the phenols levels. Cd treatment significantly reduced the activities of SOD, APX, POD, and IAA oxidase, as well as GSH level. Cd strongly increased ASA levels. ABA pretreatment counteracted Cd-induced alterations of certain antioxidants and antioxidative enzymes, e.g., remarkably rescued APX and POD activities, reduced the elevated SOD and CAT activities and ASA levels, and recovered the reduced GSH levels, caused by Cd stress. Thus, the physiological effects of the combination of ABA and Cd treatments were opposite of those obtained with Cd treatment alone, suggesting that ABA involved in the regulation of antioxidative defense systems and the alleviation of wounding- and Cd-induced oxidative stress.
authors.affiliation: School of Chemical and Biological Engineering, Lanzhou Jiaotong University, 88 West Anning Road, Lanzhou, 730070, People's Republic of China, lishweng@yahoo.com.cn.
authors.forename: Shi-Weng
authors.initials: SW
authors.lastname: Li
authors.forename: Yan
authors.initials: Y
authors.lastname: Leng
authors.forename: Lin
authors.initials: L
authors.lastname: Feng
authors.forename: Xiao-Ying
authors.initials: XY
authors.lastname: Zeng
mesh_terms: D000040:Abscisic Acid / Q000378:metabolism*; D060387:Ascorbate Peroxidases; D001205:Ascorbic Acid / Q000378:metabolism; D002104:Cadmium / Q000633:toxicity*; D002374:Catalase / Q000378:metabolism; D007887:Fabaceae / Q000378:metabolism / Q000502:physiology*; D005978:Glutathione / Q000378:metabolism; D010084:Oxidation-Reduction; D009195:Peroxidase / Q000378:metabolism; D010544:Peroxidases / Q000378:metabolism*; D018517:Plant Roots / Q000187:drug effects / Q000502:physiology; D036226:Seedlings / Q000187:drug effects / Q000378:metabolism; D012989:Soil Pollutants / Q000633:toxicity*; D013312:Stress, Physiological; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D012989:Soil Pollutants; D002104:Cadmium; D000040:Abscisic Acid; D010544:Peroxidases; D060387:Ascorbate Peroxidases; D002374:Catalase; D009195:Peroxidase; D013482:Superoxide Dismutase; D005978:Glutathione; D001205:Ascorbic Acid