title: Magnesium deficiency regulates vitamin D metabolizing enzymes and type II sodium-phosphate cotransporter mRNA expression in rats.
abstract: A magnesium (Mg) deficiency induces changes in calcium (Ca) and phosphorus (P) metabolism; however, the mechanisms responsible for these effects remain unclear. Since 1,25-dihydroxyvitamin D3 and type II sodium-phosphate (Na/Pi) cotransporters are essential regulators of Ca and P metabolism, this study examined the effects of Mg deficiency on the mRNA expression of vitamin D metabolizing enzymes (25-hydroxyvitamin D-1α-hydroxylase (1α(OH)ase) and 25-hydroxyvitamin D-24-hydroxylase (24(OH)ase)), and Na/Pi cotransporters (type IIa and IIc) in the rat kidney. Rats were divided into two groups and fed a control diet (Mg concentration: 0.05%) or a Mg-deficient diet (Mg concentration: Mg-free) for 21 days. 1α(OH)ase mRNA levels were significantly decreased in rats fed the Mg-deficient diet, while 24(OH)ase mRNA levels were significantly increased, compared to rats fed the control diet. Type IIa and IIc Na/Pi cotransporter mRNA levels in rats fed the Mg-deficient diet were significantly decreased compared to rats fed the control diet. These results suggest that Mg deficiency induces downregulation of 1α(OH)ase and type IIa and IIc Na/Pi cotransporters, and upregulation of 24(OH)ase in the kidney.
authors.affiliation: Department of Nutrition, Junior College of Tokyo University of Agriculture, Tokyo 156-8502, Japan. matsu@nodai.ac.jp
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Matsuzaki
authors.forename: Shin-ichi
authors.initials: S
authors.lastname: Katsumata
authors.forename: Yasutaka
authors.initials: Y
authors.lastname: Kajita
authors.forename: Misao
authors.initials: M
authors.lastname: Miwa
mesh_terms: D000818:Animals; D005786:Gene Expression Regulation; D008275:Magnesium Deficiency / Q000201:enzymology* / Q000235:genetics*; D008297:Male; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D050614:Sodium-Phosphate Cotransporter Proteins, Type IIb / Q000235:genetics* / Q000378:metabolism; D050615:Sodium-Phosphate Cotransporter Proteins, Type IIc / Q000235:genetics* / Q000378:metabolism; D013250:Steroid Hydroxylases / Q000235:genetics* / Q000378:metabolism; D065668:Vitamin D3 24-Hydroxylase
chemical_list: D012333:RNA, Messenger; D050614:Sodium-Phosphate Cotransporter Proteins, Type IIb; D050615:Sodium-Phosphate Cotransporter Proteins, Type IIc; D013250:Steroid Hydroxylases; C406322:vitamin D 1-alpha hydroxylase; D065668:Vitamin D3 24-Hydroxylase
keywords: Na/Pi cotransporter; magnesium deficiency; rats; vitamin D metabolizing enzyme