title: [Influence of TIEG1 on apoptosis of HL-60 cells and expression of Bcl-2/Bax].
abstract: This study was aimed to investigate the influence of TIEG1 on apoptosis of HL-60 cells and the expression of Bcl-2/Bax. Different concentration of TIEG1 were used to treat HL-60 cells, the cell growth inhibition rate was detected by MTT method. After treating HL-60 cells with 12.03 ng/ml TIEG1, cell apoptosis was detected with flow cytometry. Bcl-2 and Bax was detected with RT-PCR. The results showed that TIEG1 had inhibitory effect on HL-60 cell proliferation, and in time-and dose-dependent manners. The more obvious inhibitory effect was observed in HL-60 cells treated with TIEG1 of 12.03 ng/ml. During the course of cell apoptosis, Bax expression increased, but Bcl-2 expression decreased (P < 0.05). It is concluded that TIEG1 inhibits HL-60 cell proliferation and induces apoptosis in time and dose-dependent manners. During the course of HL-60 cells apoptosis induced by TIEG1, Bcl-2/Bax are associated with HL-60 cell apoptosis induced by TIEG1.
authors.affiliation: Department of Hematology, China Medical University, Shengyang, China.
authors.forename: Kun
authors.initials: K
authors.lastname: Yao
authors.forename: Ying
authors.initials: Y
authors.lastname: Yang
authors.forename: Rong
authors.initials: R
authors.lastname: Hu
authors.forename: Miao
authors.initials: M
authors.lastname: Miao
authors.forename: Ai-Jun
authors.initials: AJ
authors.lastname: Liao
authors.forename: Wei
authors.initials: W
authors.lastname: Yang
authors.forename: Zhuo-Gang
authors.initials: ZG
authors.lastname: Liu
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D051765:Early Growth Response Transcription Factors / Q000494:pharmacology*; D015973:Gene Expression Regulation, Leukemic; D018922:HL-60 Cells; D006801:Humans; D051741:Kruppel-Like Transcription Factors / Q000494:pharmacology*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism*; D051028:bcl-2-Associated X Protein / Q000378:metabolism*
chemical_list: C494826:BAX protein, human; D051765:Early Growth Response Transcription Factors; C098111:KLF10 protein, human; D051741:Kruppel-Like Transcription Factors; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein