title: Nanoparticle-DNA-polymer composites for hepatocellular carcinoma cell labeling, sensing, and magnetic resonance imaging.
abstract: This paper describes comparative studies and protocols in (1) self-assembling of ultrasmall superparamagnetic iron oxide nanoparticle (NP), circular plasmid DNA, and branched polyethylenimine (PEI) composites; (2) magnetofection; (3) gene delivery, (4) magnetic resonance imaging (MRI), and (5) cytotoxicity of the composites toward hepatocellular carcinoma HepG2 cells.
authors.affiliation: Department of Chemistry, The Hong Kong Baptist University, Kowloon Tong, Kowloon, Hong Kong Special Administrative Region; Institute of Creativity, The Hong Kong Baptist University, Kowloon Tong, Kowloon, Hong Kong Special Administrative Region; Institute of Molecular Functional Materials, Areas of Excellence, University Grants Committee, Hong Kong Special Administrative Region. Electronic address: cfleung@hkbu.edu.hk.
authors.forename: Ken Cham-Fai
authors.initials: KC
authors.lastname: Leung
authors.forename: Siu-Fung
authors.initials: SF
authors.lastname: Lee
authors.forename: Chi-Hin
authors.initials: CH
authors.lastname: Wong
authors.forename: Chun-Pong
authors.initials: CP
authors.lastname: Chak
authors.forename: Josie M Y
authors.initials: JM
authors.lastname: Lai
authors.forename: Xiao-Ming
authors.initials: XM
authors.lastname: Zhu
authors.forename: Yi-Xiang J
authors.initials: YX
authors.lastname: Wang
authors.forename: Kathy W Y
authors.initials: KW
authors.lastname: Sham
authors.forename: Christopher H K
authors.initials: CH
authors.lastname: Cheng
mesh_terms: D000464:Alginates / Q000737:chemistry; D000818:Animals; D006528:Carcinoma, Hepatocellular; D002470:Cell Survival; D002951:Citrates / Q000737:chemistry; D004270:DNA, Circular / Q000737:chemistry / Q000235:genetics; D004285:Dogs; D052203:Ferrosoferric Oxide / Q000737:chemistry; D049452:Green Fluorescent Proteins / Q000096:biosynthesis; D056945:Hep G2 Cells; D006801:Humans; D049410:Luciferases, Renilla / Q000096:biosynthesis; D061985:Madin Darby Canine Kidney Cells; D008279:Magnetic Resonance Imaging; D053761:Nanocomposites / Q000737:chemistry*; D010957:Plasmids / Q000737:chemistry / Q000235:genetics; D011094:Polyethyleneimine / Q000737:chemistry; D013194:Staining and Labeling; D014162:Transfection
chemical_list: D000464:Alginates; D002951:Citrates; D004270:DNA, Circular; D049452:Green Fluorescent Proteins; D011094:Polyethyleneimine; D049410:Luciferases, Renilla; D052203:Ferrosoferric Oxide
keywords: Cellular uptake; DMEM; Dulbecco’s modified Eagle medium; EDTA; FBS; FTIR; Fourier transform infrared; GFP; Gene delivery; MDCK; MRI; MTT; Madin–Darby Canine kidney; Magnetic resonance imaging; Magnetofection; NIB; NP; Nanoparticle; Neodymium–iron–boron; PBS; PEI; RLU; SPIO; Self-assembly; UV; ethylenediaminetetraacetic acid; fetal bovine serum; green fluorescence protein; magnetic resonance imaging; methylthiazolydiphenyl-tetrazolium bromide; nanoparticle; phosphate buffer saline; polyethylenimine; relative light unit; superparamagnetic iron oxide; ultraviolet