title: The engraftment and differentiation of transplanted bone marrow-derived cells in the olfactory bulb after methimazole administration.
abstract: CONCLUSIONS
  Bone marrow-derived cells can be engrafted in the olfactory bulb and a few cells can differentiate into mitral/tufted cells in the olfactory bulb.
  
  
  OBJECTIVE
  To investigate whether bone marrow-derived cells can be engrafted into the olfactory bulb and differentiate into neurons and glial cells after methimazole administration.
  
  
  METHODS
  Bone marrow of GFP (green fluorescence protein) mice was transplanted into lethally irradiated recipient mice. Immunostaining was performed to confirm the cell types of bone marrow-derived cells expressing GFP.
  
  
  RESULTS
  GFP-positive cells were observed in the olfactory bulb at 2 days after methimazole administration. The number of dendritic GFP-positive cells increased up to 30 days after methimazole administration and then decreased. Double immunostaining for GFP and Iba1 or TBX21 showed that a large population of the GFP-positive cells had characteristics of microglia/macrophages and a few cells had characteristics of mitral/tufted cells.
authors.affiliation: Department of Otolaryngology-Head & Neck Surgery, Okayama, Japan.
authors.forename: Yohei
authors.initials: Y
authors.lastname: Noda
authors.forename: Kazunori
authors.initials: K
authors.lastname: Nishizaki
authors.forename: Junko
authors.initials: J
authors.lastname: Yoshinobu
authors.forename: Yorihisa
authors.initials: Y
authors.lastname: Orita
authors.forename: Hidetsugu
authors.initials: H
authors.lastname: Tsujigiwa
authors.forename: Masao
authors.initials: M
authors.lastname: Yamada
mesh_terms: D000818:Animals; D013956:Antithyroid Agents / Q000008:administration & dosage*; D016026:Bone Marrow Transplantation; D002135:Calcium-Binding Proteins / Q000378:metabolism; D002454:Cell Differentiation / Q000502:physiology*; D005260:Female; D049452:Green Fluorescent Proteins; D045164:Mesenchymal Stem Cell Transplantation; D008713:Methimazole / Q000008:administration & dosage*; D051379:Mice; D008810:Mice, Inbred C57BL; D008840:Microfilament Proteins / Q000378:metabolism; D009830:Olfactory Bulb / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D051842:Olfactory Marker Protein / Q000378:metabolism; D009831:Olfactory Mucosa / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D020825:T-Box Domain Proteins / Q000378:metabolism; D000098815:T-bet Transcription Factor
chemical_list: C491049:Aif1 protein, mouse; D013956:Antithyroid Agents; D002135:Calcium-Binding Proteins; D008840:Microfilament Proteins; D051842:Olfactory Marker Protein; C497598:Omp protein, mouse; D020825:T-Box Domain Proteins; D000098815:T-bet Transcription Factor; D049452:Green Fluorescent Proteins; D008713:Methimazole