title: Development of an enzyme-linked immunosorbent assay for dibutyl phthalate in liquor.
abstract: A monoclonal antibody specifically recognizing dibutyl phthalate (DBP) was prepared based on a hapten (di-n-butyl-4-aminophthalate). After optimizing various parameters such as concentrations of antibody, coating antigen and composition of the assay buffer, an inhibition curve was plotted with the 50% inhibition concentration value (IC50) 33.6 ± 2.5 ng/mL. A low level of cross-reactivity (<5%) was found for other phthalate esters. Recovery tests were conducted using liquor simulant (a mixture of water and ethanol) at two fortification levels (100 ng/mL and 300 ng/mL). The recovery rates ranged from 84.7% to 94.5% with a coefficient of variation between 7.1% and 12.8%. Nine liquor samples of different alcoholic strengths were detected using the proposed measure and confirmatory analysis was performed using liquid chromatography-mass spectroscopy (LC-MS). The detection results showed good consistency between the two measures and all the data above indicated that the proposed ELISA could be applied in DBP screening.
authors.affiliation: State Key Laboratory of Food Science & Technology, School of Food Science & Technology, Jiangnan University, Wuxi 214122, China. khecho@163.com
authors.forename: Hua
authors.initials: H
authors.lastname: Kuang
authors.forename: Liqiang
authors.initials: L
authors.lastname: Liu
authors.forename: Liguang
authors.initials: L
authors.lastname: Xu
authors.forename: Wei
authors.initials: W
authors.lastname: Ma
authors.forename: Lingling
authors.initials: L
authors.lastname: Guo
authors.forename: Libing
authors.initials: L
authors.lastname: Wang
authors.forename: Chuanlai
authors.initials: C
authors.lastname: Xu
mesh_terms: D000434:Alcoholic Beverages / Q000032:analysis*; D015374:Biosensing Techniques; D003993:Dibutyl Phthalate / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005504:Food Analysis / Q000295:instrumentation*; D005506:Food Contamination / Q000032:analysis*; D007118:Immunoassay / Q000295:instrumentation*; D010968:Plasticizers / Q000032:analysis
chemical_list: D010968:Plasticizers; D003993:Dibutyl Phthalate