title: [Direct involvement of the glucose and Mg(2+)-ATP in the regulation of the GABAA-coupled Cl-,H(CO3(-) -activated Mg(2+)-ATPase of the plasma membrane from rat brain in vitro experiences].
abstract: Effect of the glucose and Mg(2+)-ATP on the coupled with CABA(A)-receptor Cl-,HCO3(-)-activated Mg(2+)-ATPase of the plasma membranes from rat brain involving from "basal" Mg(2+)-ATPase which it is activated by Cl-,HCO3(-) ions it was investigated. The glucose (1-10 mM) decreased the "basal" Mg(2+)-ATPase activity on 17% and completely eliminated the enzyme activation by 10 mM Cl(-)+2 mM HCO3(-) ions. The variety effect of the glucose and Mg(2+)-ATP on the activation of the '"basal" Mg(2+)-ATPase by variety concentrations of anions it was established. So it was found that in the presence Mg(2+)-ATP in the incubation medium > 1 mM the enzyme activation by 10 mM Cl- + 2 mM HCO ions not appear. However, in the presence of the glucose (10 mM) the inhibiting effect of the Mg(2+)-ATP on the enzyme is disappears and Cl-,HCO3(-)-ATPase activity is restored. While, in the presence of the high concentrations 40 mM Cl- + 8 mM HCO3(-) in the incubation medium the inhibiting effect of the glucose and Mg(2+)-ATP it was negligible (-20%). It was conclusion about direct involvement of the glucose and Mg(2+)-ATP in the regulation of the coupled with CABA(A)-receptor Cl-,HCO3(-)-ATPase activity of the neuronal membrane under different concentration anions in the incubation medium.
authors.affiliation: Institute of General Pathology and Pathophysiology RAMS, Moscow, Russia
authors.forename: S A
authors.initials: SA
authors.lastname: Menzikov
authors.forename: M N
authors.initials: MN
authors.lastname: Karpova
authors.forename: M V
authors.initials: MV
authors.lastname: Kalinina
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000201:enzymology*; D017301:Ca(2+) Mg(2+)-ATPase / Q000378:metabolism*; D002462:Cell Membrane / Q000201:enzymology; D002712:Chlorides / Q000378:metabolism; D005947:Glucose / Q000378:metabolism*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D011963:Receptors, GABA-A / Q000378:metabolism*
chemical_list: D002712:Chlorides; D011963:Receptors, GABA-A; D000255:Adenosine Triphosphate; D017301:Ca(2+) Mg(2+)-ATPase; C032666:magnesium-bicarbonate ATPase; D005947:Glucose