title: Kininogen 1 and insulin-like growth factor binding protein 6: candidate serum biomarkers of proliferative vitreoretinopathy.
abstract: BACKGROUND
  The aim was to validate whether kininogen 1 (KNG1) or insulin-like growth factor binding protein 6 (IGFBP-6) are serum biomarkers of proliferative vitreoretinopathy (PVR).
  
  
  METHODS
  Samples from vitreous and corresponding serum samples were collected from patients with PVR. The donor vitreous samples and serum samples from healthy volunteers and volunteers who had undergone vitrectomies for other conditions were used as controls. The samples were subsequently analysed using Western blotting (WB) and enzyme-linked immunosorbent assay.
  
  
  RESULTS
  The Western blotting outcomes indicated both IGFBP-6 and KNG1 could be specifically detected in the vitreous and serum samples of patients with PVR. The concentrations of KNG1 and IGFBP-6 were significantly higher in both vitreous and serum samples from patients with severe PVR than in the samples from patients with moderate PVR. The serum concentrations of KNG1 or IGFBP-6 had decreased by the post-vitrectomy examinations. The receiver operating characteristic (ROC) analyses when the concentrations of IGFBP-6 or KNG1 were greater than 181.4 pg/ml or 441.75 ng/ml, respectively, predicted severe PVR with both a sensitivity and specificity of over 70 per cent. When the concentrations of IGFBP-6 or KNG1 were greater than 98.5 pg/ml or 88.5 ng/ml, respectively, they predicted the PVR prognosis with both a sensitivity and specificity of 80 per cent.
  
  
  CONCLUSIONS
  KNG1 and IGFBP-6 may be candidate serum biomarkers of PVR.
authors.affiliation: Department of Ophthalmology, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai, China. jingyuchn@126.com.
authors.forename: Jing
authors.initials: J
authors.lastname: Yu
authors.forename: Runsheng
authors.initials: R
authors.lastname: Peng
authors.forename: Hui
authors.initials: H
authors.lastname: Chen
authors.forename: Chen
authors.initials: C
authors.lastname: Cui
authors.forename: Jun
authors.initials: J
authors.lastname: Ba
authors.forename: Fang
authors.initials: F
authors.lastname: Wang
mesh_terms: D000368:Aged; D015415:Biomarkers / Q000097:blood; D015153:Blotting, Western; D016039:Corneal Transplantation; D004797:Enzyme-Linked Immunosorbent Assay; D005125:Eye Banks; D015785:Eye Diseases, Hereditary / Q000097:blood / Q000175:diagnosis / Q000601:surgery; D005260:Female; D006801:Humans; D018976:Insulin-Like Growth Factor Binding Protein 6 / Q000097:blood*; D007704:Kininogens / Q000097:blood*; D008297:Male; D008875:Middle Aged; D011237:Predictive Value of Tests; D057234:Preoperative Period; D011379:Prognosis; D012163:Retinal Detachment / Q000097:blood / Q000175:diagnosis / Q000601:surgery; D012680:Sensitivity and Specificity; D014821:Vitrectomy; D018630:Vitreoretinopathy, Proliferative / Q000097:blood* / Q000175:diagnosis* / Q000601:surgery
chemical_list: D015415:Biomarkers; D018976:Insulin-Like Growth Factor Binding Protein 6; C000591034:KNG1 protein, human; D007704:Kininogens
keywords: biomarker; insulin-like growth factor binding protein; kininogen; proliferative vitreoretinopathy; rhegmatogenous retinal detachment