title: Matrix-assisted laser desorption ionization-time of flight mass spectrometry can accurately differentiate between Mycobacterium masilliense (M. abscessus subspecies bolletti) and M. abscessus (sensu stricto).
abstract: Among 36 Mycobacterium masilliense and 22 M. abscessus isolates identified by erm(41) PCR and sequencing analysis of rpoB and 23S rRNA genes, the rate of accurate differentiation between these two subspecies was 100% by cluster analysis of spectra generated by Bruker Biotyper matrix-assisted laser desorption ionization-time of flight mass spectrometry.
authors.affiliation: Department of Graduate Institute of Biomedical Sciences, Chang Gung University, Tao-Yuan, Taiwan.
authors.forename: Shih-Hua
authors.initials: SH
authors.lastname: Teng
authors.forename: Chung-Ming
authors.initials: CM
authors.lastname: Chen
authors.forename: Meng-Rui
authors.initials: MR
authors.lastname: Lee
authors.forename: Tai-Fen
authors.initials: TF
authors.lastname: Lee
authors.forename: Kun-Yi
authors.initials: KY
authors.lastname: Chien
authors.forename: Lee-Jene
authors.initials: LJ
authors.lastname: Teng
authors.forename: Po-Ren
authors.initials: PR
authors.lastname: Hsueh
mesh_terms: D001431:Bacteriological Techniques / Q000379:methods*; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D012321:DNA-Directed RNA Polymerases / Q000235:genetics; D006801:Humans; D008780:Methyltransferases / Q000235:genetics; D008969:Molecular Sequence Data; D009170:Nontuberculous Mycobacteria / Q000737:chemistry* / Q000145:classification*; D016133:Polymerase Chain Reaction; D012338:RNA, Ribosomal, 23S / Q000235:genetics; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
chemical_list: D004269:DNA, Bacterial; D012338:RNA, Ribosomal, 23S; D008780:Methyltransferases; D012321:DNA-Directed RNA Polymerases; C105078:RNA polymerase beta subunit