title: Direct coupling of thin-layer chromatography with a bioassay for the detection of estrogenic compounds: applications for effect-directed analysis.
abstract: The present study investigated the hypothesis that the coupling of high-performance thin-layer chromatography with the yeast estrogen screen (planar-YES, p-YES) can be used as a screening tool for effect-directed analysis. Therefore, the proposed method was challenged for the first time with several real samples from various origins such as sediment pore water, wastewater, and sunscreens. It was possible to detect and quantify estrogenic effects in all investigated sample types, even in the presence of demanding matrixes. Furthermore, the specific agonistic effect of the estrogen receptor activation could be detected in samples exhibiting cytotoxic effects and at cytotoxic levels of analyzed estrogenic compounds, which is not possible with the classic YES. The analysis of samples by the p-YES results in profiles of estrogenic activity. By means of this profiles samples can be compared qualitatively and quantitatively with respect to different compositions of bioactive compounds in mixtures. In conclusion, the p-YES approach seems to have a high potential to be used as a valuable screening tool for various applications in effect-directed analysis.
authors.affiliation: German Federal Institute of Hydrology, Koblenz, Germany. buchinger@bafg.de
authors.forename: Sebastian
authors.initials: S
authors.lastname: Buchinger
authors.forename: Denise
authors.initials: D
authors.lastname: Spira
authors.forename: Kathrin
authors.initials: K
authors.lastname: Bröder
authors.forename: Michael
authors.initials: M
authors.lastname: Schlüsener
authors.forename: Thomas
authors.initials: T
authors.lastname: Ternes
authors.forename: Georg
authors.initials: G
authors.lastname: Reifferscheid
mesh_terms: D001681:Biological Assay / Q000379:methods*; D002855:Chromatography, Thin Layer / Q000379:methods*; D004967:Estrogens / Q000032:analysis* / Q000302:isolation & purification*; D019015:Geologic Sediments / Q000737:chemistry; D013473:Sunscreening Agents / Q000737:chemistry; D062065:Wastewater / Q000737:chemistry
chemical_list: D004967:Estrogens; D013473:Sunscreening Agents; D062065:Wastewater