title: Effects of microRNA-106 on proliferation of gastric cancer cell through regulating p21 and E2F5.
abstract: OBJECTIVE
  To investigate the effects of miR-106b on malignant characteristics of gastric cancer cells, and explore possible mechanisms.
  
  
  METHODS
  Expression of miR-106b, p21 and E2F was determined by real-time PCR. Transfection with miR-106b mimics was conducted, and gastric cancer cells with miR-106b overexpression were obtained. Cells transfected with mimic mutants and those without transfection served as negative and blank controls, respectively. Flow cytometry and transwell assays were adopted to detect the effects of miR-106b overexpression on cell cycle, migration and invasion of gastric cancer cells.
  
  
  RESULTS
  . The expression of miR- 106b in gastric cancer cells was significantly higher than that in normal gastric mucosa cells. Furthermore, the expression level of miR-106b rose according to the degree of malignacy among the three GC cell strains (MKN- 45 > SGC-7901 > MKN-28). Overexpression of miR-106b shortened the G0/G1 phase and accelerated cell cycle progression, while reducing p21 and E2F5, without any significant effects on the capacity for migration and invasion of gastric cancer cells.
  
  
  CONCLUSIONS
  miR-106b may promote cell cycling of gastric cancer cells through regulation of p21 and E2F5 target gene expression.
authors.affiliation: Kunshan First People's Hospital, Affiliated to Jiangsu University, Kunshan, Jiangsu, China.
authors.forename: Yong-Liang
authors.initials: YL
authors.lastname: Yao
authors.forename: Xiao-Yang
authors.initials: XY
authors.lastname: Wu
authors.forename: Jian-Hong
authors.initials: JH
authors.lastname: Wu
authors.forename: Tao
authors.initials: T
authors.lastname: Gu
authors.forename: Ling
authors.initials: L
authors.lastname: Chen
authors.forename: Jin-Hua
authors.initials: JH
authors.lastname: Gu
authors.forename: Yun
authors.initials: Y
authors.lastname: Liu
authors.forename: Qing-Hui
authors.initials: QH
authors.lastname: Zhang
mesh_terms: D014408:Biomarkers, Tumor / Q000235:genetics; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics*; D050759:Cyclin-Dependent Kinase Inhibitor p21 / Q000096:biosynthesis* / Q000235:genetics; D050700:E2F5 Transcription Factor / Q000096:biosynthesis* / Q000235:genetics; D059585:G1 Phase Cell Cycle Checkpoints; D015972:Gene Expression Regulation, Neoplastic; D020868:Gene Silencing; D006801:Humans; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics; D009361:Neoplasm Invasiveness / Q000235:genetics; D011897:Random Allocation; D060888:Real-Time Polymerase Chain Reaction; D013270:Stomach / Q000473:pathology; D013274:Stomach Neoplasms / Q000235:genetics* / Q000473:pathology; D014162:Transfection
chemical_list: D014408:Biomarkers, Tumor; D050759:Cyclin-Dependent Kinase Inhibitor p21; D050700:E2F5 Transcription Factor; C092558:E2F5 protein, human; C542541:MIRN106 microRNA, human; D035683:MicroRNAs