title: Multi-drug resistance tuberculosis (MDR-TB) in Kassala State, Eastern Sudan.
abstract: This study investigates the prevalence of drug resistance and mutations in rpoB gene among Mycobacterium tuberculosis isolates from Kassala State, Sudan. In a cross-sectional study during 2011, sputum specimens (n = 90) were examined microscopically and grown cultures were identified by analysing IS6110 insertion sequence. Antimicrobial sensitivity and mutations in the rpoB gene were determined. Of the 90 specimens found which were positive for acid-fast bacilli (AFB), 65 (72.2%) showed growth of mycobacteria. Sixty (66.7%) of these were M. tuberculosis, 5 (5.6%) were rapidly growing mycobacteria and 10 (11.1%) revealed no growth. Of the 60 M. tuberculosis, 31 (51.7%) were drug resistant, including 18 multidrug resistant TB (30%), and 15 (83.3%) demonstrated mutations in the rpoB gene. Isoniazid and rifampicin revealed the highest resistance rates (64.5%, 61.3%, respectively). In conclusion, drug resistance M. tuberculosis in Kassala State was high (30%) and was found to be mainly (83.3%) due to mutations in the rpoB gene.
authors.affiliation: Department of Clinical Microbiology and Parasitology, College of Medicine, King Khalid University, PO Box 10034, Abha 61321, Saudi Arabia. mehamid2@yahoo.com
authors.forename: Ahmed A
authors.initials: AA
authors.lastname: Abdul-Aziz
authors.forename: Mogahid M
authors.initials: MM
authors.lastname: Elhassan
authors.forename: Salma A
authors.initials: SA
authors.lastname: Abdulsalam
authors.forename: Eman O
authors.initials: EO
authors.lastname: Mohammed
authors.forename: Mohamed E
authors.initials: ME
authors.lastname: Hamid
mesh_terms: D000995:Antitubercular Agents / Q000627:therapeutic use; D001426:Bacterial Proteins / Q000235:genetics; D003430:Cross-Sectional Studies; D012321:DNA-Directed RNA Polymerases; D005260:Female; D006801:Humans; D008297:Male; D009154:Mutation; D009169:Mycobacterium tuberculosis / Q000235:genetics; D015995:Prevalence; D013397:Sudan / Q000453:epidemiology; D018088:Tuberculosis, Multidrug-Resistant / Q000188:drug therapy / Q000453:epidemiology* / Q000235:genetics
chemical_list: D000995:Antitubercular Agents; D001426:Bacterial Proteins; C505805:rpoB protein, Mycobacterium tuberculosis; D012321:DNA-Directed RNA Polymerases
keywords: Kassala; MDR; Mycobacterium tuberculosis; Sudan; rpoB gene