title: Three dissimilar high fat diets differentially regulate lipid and glucose metabolism in obesity-resistant Slc:Wistar/ST rats.
abstract: Epidemiologic and ecologic studies suggest that dietary fat plays an important role in the development of obesity. Certain Wistar rat strains do not become obese when fed high-fat diets unlike others. In a preliminary study, we confirmed that Slc:Wistar/ST rats did not become obese when fed high-fat diets. The mechanisms governing the response of hepatic lipid-metabolizing enzymes to large quantities of dietary lipids consumed by obesity-resistant animals are unknown. The aim of the present study is to examine how obesity-resistant animals metabolize various types of high-fat diets and why they do not become obese. For this purpose, male Slc:Wistar/ST rats were fed a control low-fat diet (LS) or a high-fat diet containing fish oil (HF), soybean oil (HS), or lard (HL) for 4 weeks. We observed their phenotypes and determined lipid profiles in plasma and liver as well as mRNA expression levels in liver of genes related to lipid and glucose metabolism using DNA microarray and quantitative reverse transcriptase polymerase chain analyses. The body weights of all dietary groups were similar due to isocaloric intakes, whereas the weight of white adipose tissues in the LS group was significantly lower. The HF diet lowered plasma lipid levels by accelerated lipolysis in the peroxisomes and suppressed levels of very-low-density lipoprotein (VLDL) secretion. The HS diet promoted hepatic lipid accumulation by suppressed lipolysis in the peroxisomes and normal levels of VLDL secretion. The lipid profiles of rats fed the LS or HL diet were similar. The HL diet accelerated lipid and glucose metabolism.
authors.affiliation: Department of Biochemistry, School of Dentistry, Aichi-Gakuin University, 1-100 Kusumoto-cho, Chikusa-ku, Nagoya 464-8650, Japan. yokuteku@dpc.agu.ac.jp
authors.forename: Yoko
authors.initials: Y
authors.lastname: Hashimoto
authors.forename: Kazuyo
authors.initials: K
authors.lastname: Yamada
authors.forename: Hiromi
authors.initials: H
authors.lastname: Tsushima
authors.forename: Daisuke
authors.initials: D
authors.lastname: Miyazawa
authors.forename: Mayumi
authors.initials: M
authors.lastname: Mori
authors.forename: Koji
authors.initials: K
authors.lastname: Nishio
authors.forename: Takeshi
authors.initials: T
authors.lastname: Ohkubo
authors.forename: Hidehiko
authors.initials: H
authors.lastname: Hibino
authors.forename: Naoki
authors.initials: N
authors.lastname: Ohara
authors.forename: Harumi
authors.initials: H
authors.lastname: Okuyama
mesh_terms: D052436:Adipose Tissue, White / Q000187:drug effects; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D059305:Diet, High-Fat / Q000009:adverse effects*; D004041:Dietary Fats / Q000032:analysis / Q000378:metabolism / Q000493:pharmacokinetics / Q000494:pharmacology; D004435:Eating / Q000187:drug effects; D002149:Energy Intake / Q000187:drug effects; D005227:Fatty Acids / Q000032:analysis / Q000737:chemistry; D005395:Fish Oils / Q000032:analysis / Q000494:pharmacology; D005786:Gene Expression Regulation / Q000187:drug effects; D005947:Glucose / Q000378:metabolism*; D050356:Lipid Metabolism / Q000187:drug effects* / Q000235:genetics; D008079:Lipoproteins, VLDL / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000378:metabolism / Q000502:physiology; D008297:Male; D009765:Obesity / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013024:Soybean Oil / Q000032:analysis / Q000494:pharmacology
chemical_list: D004041:Dietary Fats; D005227:Fatty Acids; D005395:Fish Oils; D008079:Lipoproteins, VLDL; D013024:Soybean Oil; D005947:Glucose; C029310:lard