title: Identification of functionally key residues in AMPA receptor with a thermodynamic method.
abstract: AMPA receptor mediates the fast excitatory synaptic transmission in the central nervous system, and it is activated by the binding of glutamate that results in the opening of the transmembrane ion channel. In the present work, the thermodynamic method developed by our group was improved and then applied to identify the functionally key residues that regulate the glutamate-binding affinity of AMPA receptor. In our method, the key residues are identified as those whose perturbation largely changes the ligand binding free energy of the protein. It is found that besides the ligand binding sites, other residues distant from the binding cleft can also influence the glutamate binding affinity through a long-range allosteric regulation. These allosteric sites include the hinge region of the ligand binding cleft, the dimer interface of the ligand binding domain, the linkers between the ligand binding domain and the transmembrane domain, and the interface between the N-terminal domain and the ligand binding domain. Our calculation results are consistent with the available experimental data. The results are helpful for our understanding of the mechanism of long-range allosteric communication in the AMPA receptor and the mechanism of channel opening triggered by glutamate binding.
authors.affiliation: College of Science, Yanshan University, Qinhuangdao, China.
authors.forename: Ji Guo
authors.initials: JG
authors.lastname: Su
authors.forename: Hui Jing
authors.initials: HJ
authors.lastname: Du
authors.forename: Rui
authors.initials: R
authors.lastname: Hao
authors.forename: Xian Jin
authors.initials: XJ
authors.lastname: Xu
authors.forename: Chun Hua
authors.initials: CH
authors.lastname: Li
authors.forename: Wei Zu
authors.initials: WZ
authors.lastname: Chen
authors.forename: Cun Xin
authors.initials: CX
authors.lastname: Wang
mesh_terms: D000494:Allosteric Regulation; D001665:Binding Sites; D018360:Crystallography, X-Ray; D008024:Ligands; D008958:Models, Molecular; D018091:Receptors, AMPA / Q000737:chemistry*; D013816:Thermodynamics
chemical_list: D008024:Ligands; D018091:Receptors, AMPA