title: Circulating microRNAs as a Fingerprint for Liver Cirrhosis.
abstract: Sensitive and specific detection of liver cirrhosis is an urgent need for optimal individualized management of disease activity. Substantial studies have identified circulation miRNAs as biomarkers for diverse diseases including chronic liver diseases. In this study, we investigated the plasma miRNA signature to serve as a potential diagnostic biomarker for silent liver cirrhosis.
  
  
  
  A genome-wide miRNA microarray was first performed in 80 plasma specimens. Six candidate miRNAs were selected and then trained in CHB-related cirrhosis and controls by qPCR. A classifier, miR-106b and miR-181b, was validated finally in two independent cohorts including CHB-related silent cirrhosis and controls, as well as non-CHB-related cirrhosis and controls as validation sets, respectively.
  
  
  
  A profile of 2 miRNAs (miR-106b and miR-181b) was identified as liver cirrhosis biomarkers irrespective of etiology. The classifier constructed by the two miRNAs provided a high diagnostic accuracy for cirrhosis (AUC = 0.882 for CHB-related cirrhosis in the training set, 0.774 for CHB-related silent cirrhosis in one validation set, and 0.915 for non-CHB-related cirrhosis in another validation set).
  
  
  
  Our study demonstrated that the combined detection of miR-106b and miR-181b has a considerable clinical value to diagnose patients with liver cirrhosis, especially those at early stage.
authors.affiliation: Department of Gastroenterology, Zhongshan Hospital of Fudan University, Shanghai, China.
authors.forename: Yan-Jie
authors.initials: YJ
authors.lastname: Chen
authors.forename: Ji-Min
authors.initials: JM
authors.lastname: Zhu
authors.forename: Hao
authors.initials: H
authors.lastname: Wu
authors.forename: Jia
authors.initials: J
authors.lastname: Fan
authors.forename: Jian
authors.initials: J
authors.lastname: Zhou
authors.forename: Jie
authors.initials: J
authors.lastname: Hu
authors.forename: Qian
authors.initials: Q
authors.lastname: Yu
authors.forename: Tao-Tao
authors.initials: TT
authors.lastname: Liu
authors.forename: Lei
authors.initials: L
authors.lastname: Yang
authors.forename: Chun-Lei
authors.initials: CL
authors.lastname: Wu
authors.forename: Xiao-Ling
authors.initials: XL
authors.lastname: Guo
authors.forename: Xiao-Wu
authors.initials: XW
authors.lastname: Huang
authors.forename: Xi-Zhong
authors.initials: XZ
authors.lastname: Shen
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D008103:Liver Cirrhosis / Q000097:blood* / Q000175:diagnosis* / Q000235:genetics; D008297:Male; D035683:MicroRNAs / Q000097:blood* / Q000235:genetics; D008875:Middle Aged
chemical_list: D015415:Biomarkers; C542541:MIRN106 microRNA, human; C540193:MIrn181 microRNA, human; D035683:MicroRNAs