title: Lack of association of C-Met-N375S sequence variant with lung cancer susceptibility and prognosis.
abstract: BACKGROUND
  Previously, we identified a sequence variant (N375S) of c-Met gene, however, its association with lung cancer risk and prognosis remain undefined.
  
  
  METHODS
  We investigated the genotype distribution of the c-Met-N375S sequence variant in 206 lung cancer patients and 207 non-cancer controls in the Taiwanese population by DNA sequencing.
  
  
  RESULTS
  Lung cancer patients with variant A/G and G/G genotypes showed 1.08-fold increased cancer risk when compared to patients with the wild-type A/A genotype (95% CI, 0.60-1.91). There were no significant differences in postoperative survival between c-Met-N375S and wild-type patients. In the cell model, the c-Met-N375S cells showed a decrease in cell death upon treatment with MET inhibitor SU11274 compared to wild-type cells.
  
  
  CONCLUSIONS
  Our data suggest that the c-Met-N375S sequence variant may not play a significant role in cancer susceptibility and the prognosis of lung cancer patients. The correlation with chemoresponse of c-Met-N375S is worth further investigation in patients receiving MET therapy.
authors.affiliation: 1. Department of Chest Medicine, Chi Mei Medical Center, Tainan, Taiwan, ROC.
authors.forename: Jiunn-Min
authors.initials: JM
authors.lastname: Shieh
authors.forename: Yen-An
authors.initials: YA
authors.lastname: Tang
authors.forename: Tsung-Han
authors.initials: TH
authors.lastname: Yang
authors.forename: Chih-Yi
authors.initials: CY
authors.lastname: Chen
authors.forename: Han-Shui
authors.initials: HS
authors.lastname: Hsu
authors.forename: Yi-Hung Carol
authors.initials: YH
authors.lastname: Tan
authors.forename: Ravi
authors.initials: R
authors.lastname: Salgia
authors.forename: Yi-Ching
authors.initials: YC
authors.lastname: Wang
mesh_terms: D001483:Base Sequence; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics*; D016022:Case-Control Studies; D017931:DNA Primers; D005260:Female; D020022:Genetic Predisposition to Disease; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics* / Q000473:pathology; D008297:Male; D016133:Polymerase Chain Reaction; D011379:Prognosis; D019859:Proto-Oncogene Proteins c-met / Q000235:genetics*
chemical_list: D017931:DNA Primers; D019859:Proto-Oncogene Proteins c-met
keywords: N375S; c-Met; cancer risk; lung cancer.; prognosis