title: Quantification of microglial phagocytosis by a flow cytometer-based assay.
abstract: Microglia represent the largest population of phagocytes in the CNS and have a principal role in immune defense and inflammatory responses in the CNS. Their phagocytic activity can be studied by a variety of techniques, including a flow cytometry-based approach utilizing polystyrene latex beads. The flow cytometry-based microglial phagocytosis assay, which is presented here, offers the advantage of rapid and reliable analysis of thousands of cells in a quantitative fashion.
authors.affiliation: Hannover Medical School, Hannover, Germany.
authors.forename: Refik
authors.initials: R
authors.lastname: Pul
authors.forename: Kandiyil Prajeeth
authors.initials: KP
authors.lastname: Chittappen
authors.forename: Martin
authors.initials: M
authors.lastname: Stangel
mesh_terms: D000818:Animals; D001681:Biological Assay / Q000379:methods*; D005434:Flow Cytometry; D006801:Humans; D007150:Immunohistochemistry; D017628:Microglia / Q000166:cytology*; D010587:Phagocytosis / Q000502:physiology*