title: Parallel comparison of apheresis-collected platelet concentrates stored in four different additive solutions.
abstract: OBJECTIVE
  Partially replacing plasma with additive solutions in platelet (PLT) concentrates (PCs) may help to reduce transfusion reactions. Constituents of PLT additive solutions (PASs) have been revealed to affect the quality of PCs. Previous studies involved pairwise comparison of identical PLTs with two different PASs or multicomparison using random PLTs with three or more PASs. In this study, we performed parallel comparison using PCs from identical donors with four PASs. In addition to traditional parameters, the release of bioactive substances and plasma proteins was assessed.
  
  
  METHODS
  Platelets collected four times by apheresis from three donors were suspended in Intersol, SSP+, Composol or M-sol with 35% autologous plasma. The PC parameters, including PLT activation markers, glucose consumption, chemokines and plasma proteins, were assessed during 5-day storage.
  
  
  RESULTS
  Mean PLT volumes were decreased in SSP+, Composol and M-sol after 5-day storage, with significant differences, whereas the hypertonic shock response (HSR) was decreased only in Intersol. Glucose consumption was faster in Intersol and M-sol than in SSP+ or Composol. PLT activation, determined as CD62P, sCD62P, sCD40L and RANTES, was significantly higher in Intersol than the other three PASs. No marked change was observed in fibrinopeptide A and C3a in any PASs.
  
  
  CONCLUSIONS
  M-sol, SSP+ and Composol effectively preserved the quality of PCs. PLT activation was significantly enhanced in Intersol compared with the other three PASs. These effects seem to depend on magnesium and potassium as a constituent. Parallel comparison further verified that the PC quality largely depended on PASs but not donors.
authors.affiliation: Central Blood Institute, Blood Service Headquarters, Japanese Red Cross Society, Tokyo, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Nogawa
authors.forename: Y
authors.initials: Y
authors.lastname: Naito
authors.forename: M
authors.initials: M
authors.lastname: Chatani
authors.forename: H
authors.initials: H
authors.lastname: Onodera
authors.forename: M
authors.initials: M
authors.lastname: Shiba
authors.forename: H
authors.initials: H
authors.lastname: Okazaki
authors.forename: K
authors.initials: K
authors.lastname: Matsuzaki
authors.forename: M
authors.initials: M
authors.lastname: Satake
authors.forename: K
authors.initials: K
authors.lastname: Nakajima
authors.forename: K
authors.initials: K
authors.lastname: Tadokoro
mesh_terms: D001792:Blood Platelets / Q000378:metabolism / Q000502:physiology; D001793:Blood Preservation; D005947:Glucose / Q000378:metabolism; D006801:Humans; D015539:Platelet Activation; D010983:Plateletpheresis; D012996:Solutions
chemical_list: D012996:Solutions; D005947:Glucose
keywords: C3a; CD62P; RANTES; glucose consumption; platelet additive solution; sCD40L