title: Electrochemical magnetoimmunosensing approach for the sensitive detection of H9N2 avian influenza virus particles.
abstract: A novel electrochemical magnetoimmunosensor for fast and ultrasensitive detection of H9N2 avian influenza virus particles (H9N2 AIV) was designed based on the combination of high-efficiency immunomagnetic separation, enzyme catalytic amplification, and the biotin-streptavidin system. The reusable, homemade magneto Au electrode (M-AuE) was designed and used for the direct sensing. Immunocomplex-coated magnetic beads (IMBs) were easily accumulated on the surface of the M-AuE to obtain the catalytically reduced electrochemical signal of H2 O2 after the immunoreaction. The transducer was regenerated through a simple washing procedure, which made it possible to detect all the samples on a single electrode with higher reproducibility. The magnetic-bead-based electrochemical immunosensor showed better analytical performance than the planar-electrode-based immunosensor with the same sandwich construction. Amounts as low as 10 pg mL(-1) H9N2 AIV could be detected even in samples of chicken dung. This electrochemical magnetoimmunosensor not only provides a simple platform for the detection of the virus with high sensitivity, selectivity, and reproducibility but also shows great potential in the early diagnosis of diseases.
authors.affiliation: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, 430072, PR China.
authors.forename: Chuan-Hua
authors.initials: CH
authors.lastname: Zhou
authors.forename: Yun
authors.initials: Y
authors.lastname: Shu
authors.forename: Zheng-Yuan
authors.initials: ZY
authors.lastname: Hong
authors.forename: Dai-Wen
authors.initials: DW
authors.lastname: Pang
authors.forename: Zhi-Ling
authors.initials: ZL
authors.lastname: Zhang
mesh_terms: D000818:Animals; D001717:Birds; D055664:Electrochemical Techniques; D004566:Electrodes; D006046:Gold / Q000737:chemistry; D006735:Horseradish Peroxidase / Q000378:metabolism; D007118:Immunoassay; D053127:Influenza A Virus, H9N2 Subtype / Q000276:immunology / Q000302:isolation & purification*; D005585:Influenza in Birds / Q000473:pathology / Q000821:virology; D008280:Magnetics
chemical_list: D006046:Gold; D006735:Horseradish Peroxidase
keywords: electrochemistry; immunoassays; magnetic properties; sensors; viruses