title: Assessment of the hemolytic activity and cytotoxicity of different PEG-based solubilizing agents.
abstract: The hemolytic activity and the cytotoxicity of PEG-based solubilizing agents on Caco-2 monolayer were investigated. In vitro tests can predict the irritancy potential and the delayed toxicity of the surfactants. There were significant concentration dependent differences between the result of the MTT (3-(4,5-dimethylthiazol-2-yl))-2,5-diphenyltetrazolium bromide) test and the data of the hemolytic activity test. Our investigations show that safer and more applicable tensides can be selected in order to form a more biocompatible medicament.
authors.affiliation: Department of Pharmaceutical Technology, Faculty of Pharmacy, University of Debrecen, Hungary.
authors.forename: Z
authors.initials: Z
authors.lastname: Ujhelyi
authors.forename: E
authors.initials: E
authors.lastname: Róka
authors.forename: F
authors.initials: F
authors.lastname: Fenyvesi
authors.forename: P
authors.initials: P
authors.lastname: Fehér
authors.forename: J
authors.initials: J
authors.lastname: Váradi
authors.forename: K
authors.initials: K
authors.lastname: Réti-Nagy
authors.forename: M
authors.initials: M
authors.lastname: Vecsernyés
authors.forename: I
authors.initials: I
authors.lastname: Bácskay
mesh_terms: D018938:Caco-2 Cells; D002470:Cell Survival / Q000187:drug effects*; D004396:Coloring Agents; D004912:Erythrocytes / Q000187:drug effects; D005079:Excipients; D006461:Hemolysis / Q000187:drug effects*; D006801:Humans; D066298:In Vitro Techniques; D011092:Polyethylene Glycols / Q000494:pharmacology*; D012997:Solvents; D013778:Tetrazolium Salts; D013844:Thiazoles
chemical_list: D004396:Coloring Agents; D005079:Excipients; D012997:Solvents; D013778:Tetrazolium Salts; D013844:Thiazoles; C022131:cremophor; D011092:Polyethylene Glycols; C022616:thiazolyl blue