title: Application of gas chromatography-mass spectrometry/combustion/isotope ratio mass spectrometry (GC-MS/C/IRMS) to detect the abuse of 17β-estradiol in cattle.
abstract: Although the ability to differentiate between endogenous steroids and synthetic homologues on the basis of their (13)C/(12)C isotopic ratio has been known for over a decade, this technique has been scarcely implemented for food safety purposes. In this study, a method was developed using gas chromatography-mass spectrometry/combustion/isotope ratio mass spectrometry (GC-MS/C/IRMS) to demonstrate the abuse of 17β-estradiol in cattle, by comparison of the (13)C/(12)C ratios of the main metabolite 17α-estradiol and an endogenous reference compound (ERC), 5-androstene-3β,17α-diol, in bovine urine. The intermediate precisions were determined as 0.46 and 0.26‰ for 5-androstene-3β,17α-diol and 17α-estradiol, respectively. This is, to the authors' knowledge, the first reported use of GC-MS/C/IRMS for the analysis of steroid compounds for food safety issues.
authors.affiliation: Federal Agency for the Safety of the Food Chain, Directorate General Laboratories, Brussels, Belgium. geert.janssens@favv.be
authors.forename: Geert
authors.initials: G
authors.lastname: Janssens
authors.forename: Sven
authors.initials: S
authors.lastname: Mangelinckx
authors.forename: Dirk
authors.initials: D
authors.lastname: Courtheyn
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Prévost
authors.forename: Geert
authors.initials: G
authors.lastname: De Poorter
authors.forename: Norbert
authors.initials: N
authors.lastname: De Kimpe
authors.forename: Bruno
authors.initials: B
authors.lastname: Le Bizec
mesh_terms: D000818:Animals; D002417:Cattle; D004958:Estradiol / Q000737:chemistry* / Q000652:urine; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D008297:Male; D013058:Mass Spectrometry / Q000379:methods*; D015813:Substance Abuse Detection / Q000379:methods / Q000662:veterinary*
chemical_list: D004958:Estradiol