title: Live cell imaging of cytoplasmic Ca2+ dynamics in Arabidopsis guard cells.
abstract: This protocol describes a classical method for measuring cytoplasmic Ca(2+) dynamics in Arabidopsis thaliana guard cells. The Förster (fluorescence) resonance energy transfer (FRET)-based genetically modified Ca(2+) indicator Yellow Cameleon YC3.6, under the control of the guard cell-specific promoter GC1, is used for Ca(2+) measurements.
authors.affiliation: Institute of Plant Biology and Biotechnology, University of Münster, 48149 Münster, Germany.
authors.forename: Smrutisanjita
authors.initials: S
authors.lastname: Behera
authors.forename: Jörg
authors.initials: J
authors.lastname: Kudla
mesh_terms: D017360:Arabidopsis / Q000737:chemistry*; D002118:Calcium / Q000032:analysis*; D002135:Calcium-Binding Proteins / Q000032:analysis / Q000235:genetics; D002413:Cations, Divalent / Q000032:analysis; D003593:Cytoplasm / Q000737:chemistry*; D017930:Genes, Reporter; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D008853:Microscopy / Q000379:methods*; D013194:Staining and Labeling / Q000379:methods
chemical_list: D002135:Calcium-Binding Proteins; D002413:Cations, Divalent; C495639:yellow cameleon; D002118:Calcium