title: Real Time PCR to detect and differentiate Campylobacter fetus subspecies fetus and Campylobacter fetus subspecies venerealis.
abstract: Bovine venereal campylobacter infection, caused by Campylobacter fetus venerealis, is of significant economic importance to the livestock industry. Unfortunately, the successful detection and discrimination of C. fetus venerealis from C. fetus fetus continue to be a limitation throughout the world. There are several publications warning of the problem with biotyping methods as well as with recent molecular based assays. In this study, assessed on 1071 isolates, we report on the successful development of two Real Time SYBR® Green PCR assays that will allow for the detection and discrimination of C. fetus fetus and C. fetus venerealis. The sensitivity reported here for the C. fetus (CampF4/R4) and the C. fetus venerealis (CampF7/R7) specific PCR assays are 100% and 98.7% respectively. The specificity for these same PCR assays are 99.6% and 99.8% respectively.
authors.affiliation: Animal Health and Veterinary Laboratories Agency Starcross, Exeter, United Kingdom. Adrian.McGoldrick@AHVLA.gsi.gov.uk
authors.forename: A
authors.initials: A
authors.lastname: McGoldrick
authors.forename: J
authors.initials: J
authors.lastname: Chanter
authors.forename: S
authors.initials: S
authors.lastname: Gale
authors.forename: J
authors.initials: J
authors.lastname: Parr
authors.forename: M
authors.initials: M
authors.lastname: Toszeghy
authors.forename: K
authors.initials: K
authors.lastname: Line
mesh_terms: D000818:Animals; D002169:Campylobacter Infections / Q000382:microbiology / Q000662:veterinary; D002168:Campylobacter fetus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D002417:Cattle; D005456:Fluorescent Dyes; D058889:Molecular Typing / Q000379:methods*; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D012756:Sheep
chemical_list: D005456:Fluorescent Dyes
keywords: Campylobacter; Real time polymerase chain reaction (PCR); fetus fetus; fetus venerealis