title: [Accuracy of Etest method to study Campylobacter spp. susceptibility to erythromycin, ciprofloxacin and tetracycline].
abstract: BACKGROUND
  In industrialized countries Campylobacter jejuni is the enteropathogen most frequently isolated from the feces of patients with gastroenteritis. The Etest accuracy to categorize Campylobacter spp. susceptibility to erythromycin, ciprofloxacin and tetracycline was evaluated.
  
  
  METHODS
  Ninety strains were studied. The Etest® was performed following the manufacturer's instructions on commercial plates of Mueller-Hinton blood. The breakpoints were those recommended by the Clinical Laboratory Standards Institute (CLSI) for broth microdilution. The gold standard was the broth microdilution method as recommended by CLSI.
  
  
  RESULTS
  The Etest agreement with the reference method was 100%, 97% and 98% for erythromycin, ciprofloxacin and tetracycline, respectively. No major or very major errors were found.
  
  
  CONCLUSIONS
  The Etest results are equivalent to those obtained using the gold standard. The Etest is a valid method to determine susceptibility to tetracycline. It is also a suitable method to categorize strains classified as non-resistant to erythromycin and ciprofloxacin by the diffusion method.
authors.affiliation: Servicio de Microbiología, Hospital de Móstoles, Madrid. Spain.
authors.forename: Nuria
authors.initials: N
authors.lastname: Sanz-Rodríguez
authors.forename: María Teresa
authors.initials: MT
authors.lastname: Pérez-Pomata
authors.forename: Olha
authors.initials: O
authors.lastname: Stelmakh
authors.forename: David
authors.initials: D
authors.lastname: Pérez-Boto
authors.forename: José Luis
authors.initials: JL
authors.lastname: Gómez-Garcés
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002167:Campylobacter / Q000187:drug effects*; D002169:Campylobacter Infections / Q000382:microbiology; D002939:Ciprofloxacin / Q000494:pharmacology*; D003470:Culture Media; D024881:Drug Resistance, Bacterial; D004917:Erythromycin / Q000494:pharmacology*; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods*; D012015:Reference Standards; D015203:Reproducibility of Results; D013752:Tetracycline / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D003470:Culture Media; D002939:Ciprofloxacin; D004917:Erythromycin; D013752:Tetracycline