title: Epidermal stem cells manipulated by pDNA-VEGF165/CYD-PEI nanoparticles loaded gelatin/β-TCP matrix as a therapeutic agent and gene delivery vehicle for wound healing.
abstract: The success of gene therapy largely relies on a safe and effective gene delivery system. The objective of this study is to design a highly efficient system for the transfection of epidermal stem cells (ESCs) and investigate the transfected ESCs (TESCs) as a therapeutic agent and gene delivery reservoir for wound treatment. As a nonviral vector, β-cyclodextrin-linked polyethylenimines (CYD-PEI) was synthesized by linking β-cyclodextrin with polyethylenimines (600 Da). Gelatin scaffold incorporating β-tricalcium phosphate (β-TCP) was utilized as a substrate for the culture and transfection of ESCs. With the CYD-PEI/pDNA-VEGF165 polyplexes incorporated gelatin/β-TCP scaffold based 3D transfection system, prolonged VEGF expression with a higher level was obtained at day 7 in ESCs than those in two-dimensional plates. Topical application of the TESCs significantly accelerated the skin re-epithelization, dermal collagen synthesis, and hair follicle regeneration. It also exhibited a potential in scar inhibition by regulating the distribution of different types of collagen. In contrast to ESCs, an additive capacity in stimulating angiogenesis at the wound site was observed in the TESCs. The present study provides a basis for the TESCs as a promising therapeutic agent and gene delivery reservoir for wound therapy.
authors.affiliation: Institute of Pharmaceutics, College of Pharmaceutical Sciences, Zhejiang University , Hangzhou, Zhejiang, 310058, PR China.
authors.forename: Li-Hua
authors.initials: LH
authors.lastname: Peng
authors.forename: Wei
authors.initials: W
authors.lastname: Wei
authors.forename: Xiao-Tian
authors.initials: XT
authors.lastname: Qi
authors.forename: Ying-Hui
authors.initials: YH
authors.lastname: Shan
authors.forename: Fang-Jun
authors.initials: FJ
authors.lastname: Zhang
authors.forename: Xi
authors.initials: X
authors.lastname: Chen
authors.forename: Qian-Ying
authors.initials: QY
authors.lastname: Zhu
authors.forename: Lian
authors.initials: L
authors.lastname: Yu
authors.forename: Wen-Quan
authors.initials: WQ
authors.lastname: Liang
authors.forename: Jian-Qing
authors.initials: JQ
authors.lastname: Gao
mesh_terms: D000818:Animals; D002130:Calcium Phosphates / Q000737:chemistry*; D002478:Cells, Cultured; D000078404:Epidermal Cells; D005780:Gelatin / Q000737:chemistry*; D018014:Gene Transfer Techniques; D015316:Genetic Therapy; D053758:Nanoparticles / Q000737:chemistry; D011094:Polyethyleneimine / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism* / Q000502:physiology; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics*; D014945:Wound Healing / Q000502:physiology / Q000528:radiation effects*; D047392:beta-Cyclodextrins / Q000737:chemistry*
chemical_list: D002130:Calcium Phosphates; D042461:Vascular Endothelial Growth Factor A; D047392:beta-Cyclodextrins; C485817:beta-tricalcium phosphate; C467498:vascular endothelial growth factor A, rat; D005780:Gelatin; D011094:Polyethyleneimine; C031215:betadex