title: Sauchinone, a lignan from Saururus chinensis, protects human skin keratinocytes against ultraviolet B-induced photoaging by regulating the oxidative defense system.
abstract: Ultraviolet (UV) radiation from sunlight induces matrix metalloproteinase (MMP) expression, which are responsible for collagenous extracellular matrix proteins breakdown in skin, causing photoaging. Sauchinone is reported to have various bioactivity such as antioxidative, hepatoprotective, and anti-inflammatory effects. In the present study, we investigated the protective effect of sauchinone against UVB (50 mJ/cm(2))-induced photoaging in HaCaT human epidermal keratinocytes. Sauchinone, at 5-40 µM, significantly protected keratinocytes against UVB-induced damage as assessed by cell viability and toxicity assay. Additionally, sauchinone, at 20-40 µM, prevented the upregulation of MMP-1 proteins and reduction of type 1 collagen induced by UVB. Other assays revealed that, in keratinocytes, sauchinone decreased reactive oxygen species (ROS) production and increased glutathione levels and heme oxygenase-1. Sauchinone also inhibited UVB-induced phosphorylation of mitogen-activated protein kinase (MAPK) signaling pathways. These results demonstrated that sauchinone protects skin keratinocytes through inhibition of extracellular signal-regulated kinase, c-Jun N-terminal kinase, and p38 MAPK signaling via upregulation of oxidative defense enzymes.
authors.affiliation: Department of Life and Nanopharmaceutical Science and Kyung Hee East-West Pharmaceutical Research Institute, Kyung Hee University, Seoul 130–701, Republic of Korea.
authors.forename: Gunhyuk
authors.initials: G
authors.lastname: Park
authors.forename: Hyo Geun
authors.initials: HG
authors.lastname: Kim
authors.forename: Yeomoon
authors.initials: Y
authors.lastname: Sim
authors.forename: Sang Hyun
authors.initials: SH
authors.lastname: Sung
authors.forename: Myung Sook
authors.initials: MS
authors.lastname: Oh
mesh_terms: D001578:Benzopyrans / Q000302:isolation & purification / Q000494:pharmacology*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D024042:Collagen Type I / Q000378:metabolism; D004149:Dioxoles / Q000302:isolation & purification / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D006801:Humans; D015603:Keratinocytes / Q000187:drug effects* / Q000201:enzymology / Q000528:radiation effects; D020781:Matrix Metalloproteinase 1 / Q000096:biosynthesis; D015394:Molecular Structure; D011837:Radiation-Protective Agents / Q000302:isolation & purification / Q000494:pharmacology*; D032134:Saururaceae / Q000737:chemistry*; D015595:Skin Aging / Q000187:drug effects*; D014466:Ultraviolet Rays / Q000009:adverse effects*
chemical_list: D001578:Benzopyrans; D024042:Collagen Type I; D004149:Dioxoles; D011837:Radiation-Protective Agents; C410958:sauchinone; C522360:MMP1 protein, human; D020781:Matrix Metalloproteinase 1