title: Coherent Control in Multiphoton Fluorescence Imaging.
abstract: In multiphoton fluorescence laser-scanning microscopy ultrafast laser pulses,i.e.light pulses having pulse-width ≤ 1picosecond (1 ps= 10-12s), are commonly used to circumvent the low multiphoton absorption cross-sections of common fluorophores. Starting with a discussion on how amplitude modulation of ultrashort pulse-train enhances the two-photon fluorescence providing deep insight into laser-induced photo-thermal damage, the effect of controlling time lag between phase-locked laser pulses on imaging is described. In addition, the prospects of laser pulse-shaping in signal enhancement (by temporal pulse-compression at the sample) and selective excitation of fluorophores (by manipulating the phase and/or amplitude of different frequency components within the pulse) are discussed with promising future applications lying ahead.
authors.affiliation: Deptartment of Chemistry, Indian Institute of Technology Kanpur, UP - 208016, India.
authors.forename: Arijit Kumar
authors.initials: AK
authors.lastname: De
authors.forename: Debabrata
authors.initials: D
authors.lastname: Goswami
keywords: Coherent control; amplitude modulation; multiphoton imaging; pulse shaping; pump-probe spectroscopy; time-domain control; ultrafast imaging; ultrafast laser pulses