title: Combined genomics and experimental analyses of respiratory characteristics of Shewanella putrefaciens W3-18-1.
abstract: It has previously been shown that the Shewanella putrefaciens W3-18-1 strain produces remarkably high current in microbial fuel cells (MFCs) and can form magnetite at 0°C. To explore the underlying mechanisms, we developed a genetic manipulation method by deleting the restriction-modification system genes of the SGI1 (Salmonella genome island 1)-like prophage and analyzed the key genes involved in bacterial respiration. W3-18-1 has less respiratory flexibility than the well-characterized S. oneidensis MR-1 strain, as it possesses fewer cytochrome c genes and lacks the ability to oxidize sulfite or reduce dimethyl sulfoxide (DMSO) and timethylamine oxide (TMAO). W3-18-1 lacks the hydrogen-producing Fe-only hydrogenase, and the hydrogen-oxidizing Ni-Fe hydrogenase genes were split into two separate clusters. Two periplasmic nitrate reductases (NapDAGHB and NapDABC) were functionally redundant in anaerobic growth of W3-18-1 with nitrate as the electron acceptor, though napDABC was not regulated by Crp. Moreover, nitrate respiration started earlier in W3-18-1 than in MR-1 (with NapDAGHB only) under microoxic conditions. These results indicate that Shewanella putrefaciens W3-18-1 is well adapted to habitats with higher oxygen levels. Taken together, the results of this study provide valuable insights into bacterial genome evolution.
authors.affiliation: Institute for Environmental Genomics and Department of Microbiology and Plant Biology, University of Oklahoma, Norman, Oklahoma, USA.
authors.forename: Dongru
authors.initials: D
authors.lastname: Qiu
authors.forename: Hehong
authors.initials: H
authors.lastname: Wei
authors.forename: Qichao
authors.initials: Q
authors.lastname: Tu
authors.forename: Yunfeng
authors.initials: Y
authors.lastname: Yang
authors.forename: Ming
authors.initials: M
authors.lastname: Xie
authors.forename: Jingrong
authors.initials: J
authors.lastname: Chen
authors.forename: Mark H
authors.initials: MH
authors.lastname: Pinkerton
authors.forename: Yili
authors.initials: Y
authors.lastname: Liang
authors.forename: Zhili
authors.initials: Z
authors.lastname: He
authors.forename: Jizhong
authors.initials: J
authors.lastname: Zhou
mesh_terms: D004579:Electron Transport; D053858:Metabolic Networks and Pathways / Q000235:genetics*; D009566:Nitrates / Q000378:metabolism; D010084:Oxidation-Reduction; D010100:Oxygen / Q000378:metabolism*; D020614:Shewanella putrefaciens / Q000235:genetics* / Q000378:metabolism*
chemical_list: D009566:Nitrates; D010100:Oxygen