title: Antioxidant and anti-aging activities of mycelial polysaccharides from Lepista sordida.
abstract: The intracellular polysaccharides (CLSP) were extracted from Lepista sordida mycelium in submerged culture followed by concentration and ethanol precipitation. The antioxidant activities of CLSP were evaluated both in vitro and in vivo. In vitro antioxidant assay, CLSP had noticeable scavenging activities on superoxide anion, hydroxyl radical and 2,2-diphenyl-1-picryl-hydrazyl (DPPH) radical. For antioxidant testing in vivo, different doses of CLSP were orally administrated over a period of 6 weeks in a D-galactose induced aged mice model. As results, CLSP significantly inhibited the formation of malondialdehyde (MDA) and raised the activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in mice brains and serums in a dose-dependent manner. The results provide a reference for large-scale production of CLSP by submerged fermentation and suggested that CLSP had potent antioxidant activity and could be explored as a potential dietary supplement to retard aging and attenuate age-related diseases in humans.
authors.affiliation: College of Food Science, Northeast Agricultural University, Mucai Street No. 59, Xiangfang District, Harbin 150030, PR China.
authors.forename: Weiqi
authors.initials: W
authors.lastname: Zhong
authors.forename: Ning
authors.initials: N
authors.lastname: Liu
authors.forename: Yanguang
authors.initials: Y
authors.lastname: Xie
authors.forename: Yanming
authors.initials: Y
authors.lastname: Zhao
authors.forename: Xiao
authors.initials: X
authors.lastname: Song
authors.forename: Weiming
authors.initials: W
authors.lastname: Zhong
mesh_terms: D000363:Agaricales / Q000737:chemistry*; D000375:Aging / Q000187:drug effects*; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001921:Brain / Q000187:drug effects / Q000378:metabolism; D005260:Female; D016166:Free Radical Scavengers / Q000494:pharmacology; D062610:Fungal Polysaccharides / Q000494:pharmacology*; D005979:Glutathione Peroxidase / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D051379:Mice; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D000975:Antioxidants; D016166:Free Radical Scavengers; D062610:Fungal Polysaccharides; D008315:Malondialdehyde; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase
keywords: Anti-aging; Antioxidant; Extraction; Lepista sordida; Mycelial polysaccharides; Oxidative stress