title: Identification of a receptor-binding domain in the S protein of the novel human coronavirus Middle East respiratory syndrome coronavirus as an essential target for vaccine development.
abstract: A novel human Middle East respiratory syndrome coronavirus (MERS-CoV) caused outbreaks of severe acute respiratory syndrome (SARS)-like illness with a high mortality rate, raising concerns of its pandemic potential. Dipeptidyl peptidase-4 (DPP4) was recently identified as its receptor. Here we showed that residues 377 to 662 in the S protein of MERS-CoV specifically bound to DPP4-expressing cells and soluble DPP4 protein and induced significant neutralizing antibody responses, suggesting that this region contains the receptor-binding domain (RBD), which has a potential to be developed as a MERS-CoV vaccine.
authors.affiliation: Lindsley F Kimball Research Institute, New York Blood Center, New York, New York, USA.
authors.forename: Lanying
authors.initials: L
authors.lastname: Du
authors.forename: Guangyu
authors.initials: G
authors.lastname: Zhao
authors.forename: Zhihua
authors.initials: Z
authors.lastname: Kou
authors.forename: Cuiqing
authors.initials: C
authors.lastname: Ma
authors.forename: Shihui
authors.initials: S
authors.lastname: Sun
authors.forename: Vincent K M
authors.initials: VK
authors.lastname: Poon
authors.forename: Lu
authors.initials: L
authors.lastname: Lu
authors.forename: Lili
authors.initials: L
authors.lastname: Wang
authors.forename: Asim K
authors.initials: AK
authors.lastname: Debnath
authors.forename: Bo-Jian
authors.initials: BJ
authors.lastname: Zheng
authors.forename: Yusen
authors.initials: Y
authors.lastname: Zhou
authors.forename: Shibo
authors.initials: S
authors.lastname: Jiang
mesh_terms: D000818:Animals; D057134:Antibodies, Neutralizing / Q000097:blood; D000914:Antibodies, Viral / Q000097:blood; D001665:Binding Sites; D002460:Cell Line; D017934:Coronavirus / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D018352:Coronavirus Infections / Q000821:virology; D018819:Dipeptidyl Peptidase 4 / Q000378:metabolism; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D017434:Protein Structure, Tertiary; D011991:Receptors, Virus / Q000378:metabolism; D012141:Respiratory Tract Infections / Q000821:virology; D064370:Spike Glycoprotein, Coronavirus / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D014765:Viral Vaccines / Q000235:genetics / Q000276:immunology
chemical_list: D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D011991:Receptors, Virus; D064370:Spike Glycoprotein, Coronavirus; D014765:Viral Vaccines; C042807:DPP4 protein, human; D018819:Dipeptidyl Peptidase 4