title: Characterization of plant-growth-promoting effects and concurrent promotion of heavy metal accumulation in the tissues of the plants grown in the polluted soil by Burkholderia strain LD-11.
abstract: Plant-growth-promoting (PGP) bacteria especially with the resistance to multiple heavy metals are helpful to phytoremediation. Further development of PGP bacteria is very necessary because of the extreme diversity of plants, soils, and heavy metal pollution. A Burkholderia sp. strain, numbered LD-11, was isolated, which showed resistances to multiple heavy metals and antibiotics. It can produce indole-3-acetic acid, 1-aminocyclopropane-1-carboxylic acid deaminase and siderophores. Inoculation with the LD-11 improved germination of seeds of the investigated vegetable plants in the presence of Cu, promoted elongation of roots and hypocotyledonary axes, enhanced the dry weights of the plants grown in the soils polluted with Cu and/or Pb, and increased activity of the soil urease and the rhizobacteria diversity. Inoculation with the LD-11 significantly enhanced Cu and/or Pb accumulation especially in the roots of the plants grown in the polluted soils. Notably, LD-11 could produce siderophores in the presence of Cu. Conclusively, the PGP effects and concurrent heavy metal accumulation in the plant tissues results from combined effects of the above-mentioned multiple factors. Cu is an important element that represses production of the siderophore by the bacteria. Phytoremediation by synergistic use of the investigated plants and the bacterial strain LD-11 is a phytoextraction process.
authors.affiliation: State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, College of Life Science and Technology, Key Laboratory of Ministry of Education for Microbial and Plant Genetic Engineering, Guangxi University, Nanning, Guangxi, China.
authors.forename: Gui-Hai
authors.initials: GH
authors.lastname: Huang
authors.forename: Hui-Hui
authors.initials: HH
authors.lastname: Tian
authors.forename: Hai-Ying
authors.initials: HY
authors.lastname: Liu
authors.forename: Xian-Wei
authors.initials: XW
authors.lastname: Fan
authors.forename: Yu
authors.initials: Y
authors.lastname: Liang
authors.forename: You-Zhi
authors.initials: YZ
authors.lastname: Li
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000378:metabolism; D001673:Biodegradation, Environmental; D018533:Biomass; D001937:Brassica / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism / Q000382:microbiology*; D019117:Burkholderia / Q000187:drug effects / Q000302:isolation & purification / Q000502:physiology*; D019755:Carbon-Carbon Lyases / Q000378:metabolism; D003300:Copper / Q000378:metabolism* / Q000494:pharmacology; D018525:Germination; D007210:Indoleacetic Acids / Q000378:metabolism; D007854:Lead / Q000378:metabolism* / Q000494:pharmacology; D008906:Mining; D009149:Mustard Plant / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism / Q000382:microbiology; D018517:Plant Roots / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism / Q000382:microbiology; D012231:Rhizobium / Q000254:growth & development; D058441:Rhizosphere; D012639:Seeds / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism / Q000382:microbiology; D017262:Siderophores; D012987:Soil; D012989:Soil Pollutants / Q000378:metabolism* / Q000494:pharmacology; D014510:Urease / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D007210:Indoleacetic Acids; D017262:Siderophores; D012987:Soil; D012989:Soil Pollutants; D007854:Lead; C030737:indoleacetic acid; D003300:Copper; D014510:Urease; C033634:1-aminocyclopropane-1-carboxylate deaminase; D019755:Carbon-Carbon Lyases