title: UVB irradiation-induced dysregulation of plasma membrane calcium ATPase1 and intracellular calcium homeostasis in human lens epithelial cells.
abstract: Ultraviolet B (UVB) could lead to the apoptosis of human lens epithelial cell and be hypothesized to be one of the important factors of cataractogenesis. In the human lens, Ca(2+)-ATPase is a major determinant of calcium homeostasis. Plasma membrane calcium ATPase1 (PMCA1) is a putative "housekeeping" isoform and is widely expressed in all tissues and cells, which plays an important role in calcium homeostasis. However, the effects of UVB-irradiation on the expression of PMCA1 and the cellular calcium homeostasis are still unclear. In the present study, we cultured human lens epithelial cells (HLE B-3) in vitro and investigated the effects of UVB irradiation on the expression of PMCA1 and the intracellular calcium homeostasis using real-time cell electronic sensing system, flow cytometry, fluo-3/AM probes, real-time quantitative PCR, and enzyme-linked immunosorbent assay techniques. We found that UVB irradiation could induce human lens epithelial cell death, cause intracellular calcium ion (Ca(2+)) elevation, inhibit Ca(2+)-ATPase activity and decrease the expression of PMCA1 at gene and protein levels, suggesting that the downregulation of PMCA1 and the disruption of calcium homeostasis may play important roles in UVB-induced HLE B-3 cell apoptosis.
authors.affiliation: Shandong University of Traditional Chinese Medicine, Jinan, 250002, China.
authors.forename: Qiuxin
authors.initials: Q
authors.lastname: Wu
authors.forename: Dadong
authors.initials: D
authors.lastname: Guo
authors.forename: Hongsheng
authors.initials: H
authors.lastname: Bi
authors.forename: Daoguang
authors.initials: D
authors.lastname: Wang
authors.forename: Yuxiang
authors.initials: Y
authors.lastname: Du
mesh_terms: D017304:Annexin A5 / Q000378:metabolism; D002118:Calcium / Q000378:metabolism*; D002460:Cell Line; D004797:Enzyme-Linked Immunosorbent Assay; D004847:Epithelial Cells / Q000201:enzymology* / Q000528:radiation effects*; D005453:Fluorescence; D006706:Homeostasis / Q000528:radiation effects*; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D017665:Hydroxyl Radical / Q000378:metabolism; D042541:Intracellular Space / Q000378:metabolism / Q000528:radiation effects; D007908:Lens, Crystalline / Q000166:cytology*; D053078:Membrane Potential, Mitochondrial / Q000528:radiation effects; D053499:Plasma Membrane Calcium-Transporting ATPases / Q000235:genetics / Q000378:metabolism*; D011419:Propidium / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D014466:Ultraviolet Rays
chemical_list: C506952:ATP2B1 protein, human; D017304:Annexin A5; D017382:Reactive Oxygen Species; D017665:Hydroxyl Radical; D011419:Propidium; D006861:Hydrogen Peroxide; D053499:Plasma Membrane Calcium-Transporting ATPases; D002118:Calcium