title: Flow-injection post-chemiluminescence method for the determination of palmatine.
abstract: A post-chemiluminescence (PCL) phenomenon was observed when palmatine was injected in a mixture of N-bromosuccinimide (NBS) and alkaline dichlorofluorescein (DCF) after the chemiluminescence (CL) reaction of NBS-alkaline DCF had finished. Based on the PCL reaction, a rapid and sensitive method for the determination of palmatine was established. Under optimum conditions, the CL intensity was linear, with the concentration of palmatine in the range of 5.0 × 10(-9) to 1.0 × 10(-6) M. The detection limit was 6.0 × 10(-10) M for palmatine. The relative standard deviation for 11 parallel measurements of 1.0 × 10(-7) M palmatine was 1.5%. This method was applied to the determination of palmatine in pharmaceutical samples and biological fluids, with satisfactory results. The possible reaction mechanism is discussed briefly.
authors.affiliation: School of Chemistry and Material Science, Shanxi Normal University, Linfen 041004, People's Republic of China. hsq@dns.sxnu.edu.cn
authors.forename: Suqin
authors.initials: S
authors.lastname: Han
authors.forename: Bei
authors.initials: B
authors.lastname: Wei
authors.forename: Fang
authors.initials: F
authors.lastname: Hao
mesh_terms: D001600:Berberine Alkaloids / Q000097:blood* / Q000737:chemistry / Q000652:urine*; D045424:Complex Mixtures / Q000097:blood* / Q000737:chemistry / Q000652:urine*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D017022:Flow Injection Analysis / Q000295:instrumentation* / Q000379:methods; D008163:Luminescent Measurements / Q000295:instrumentation* / Q000379:methods
chemical_list: D001600:Berberine Alkaloids; D045424:Complex Mixtures; C005413:palmatine