title: Molecular cloning, sequencing and structural studies of granulocyte-macrophage colony-stimulating factor (GM-CSF) from Indian water buffalo (Bubalus bubalis).
abstract: Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a cytokine that is essential for growth and development of progenitors of granulocytes and monocytes/macrophages. In this study, we report molecular cloning, sequencing and characterization of GM-CSF from Indian water buffalo, Bubalus bubalis. In addition, we performed sequence and structural analysis for buffalo GM-CSF. Buffalo GM-CSF has been compared with 17 mammalian GM-CSFs using multiple sequence alignment and phylogenetic tree. Three-dimensional model for buffalo GM-CSF and human receptor complex was built using homology modelling to study cross-reactivity between two species. Detailed analysis was performed to study GM-CSF interface and various interactions at the interface.
authors.affiliation: Bioscience Core Lab, King Abdullah University of Science and Technology, Thuwal, Saudi Arabia.
authors.forename: Thennarasu
authors.initials: T
authors.lastname: Sugumar
authors.forename: Ganesan
authors.initials: G
authors.lastname: Pugalenthi
authors.forename: Murugesan
authors.initials: M
authors.lastname: Harishankar
authors.forename: G
authors.initials: G
authors.lastname: Dhinakar Raj
mesh_terms: D000818:Animals; D002020:Buffaloes / Q000235:genetics*; D003001:Cloning, Molecular; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000737:chemistry / Q000235:genetics*; D017422:Sequence Analysis, DNA
chemical_list: D016178:Granulocyte-Macrophage Colony-Stimulating Factor