title: Structural foundations for the O2 resistance of Desulfomicrobium baculatum [NiFeSe]-hydrogenase.
abstract: This study shows how the NiFeSe site of an anaerobically purified O2-resistant hydrogenase reacts with air to give a seleninate as the first product. Less oxidized states of the active site are readily reduced in the presence of X-rays. Reductive enzyme activation requires an efficient pathway for water escape.
authors.affiliation: Metalloproteins Unit, Institut de Biologie Structurale J.P. Ebel, CEA, CNRS, Université Grenoble Alpes UMR 5075, 41 rue Jules Horowitz, 38027 Grenoble, France. anne.volbeda@ibs.fr
authors.forename: Anne
authors.initials: A
authors.lastname: Volbeda
authors.forename: Patricia
authors.initials: P
authors.lastname: Amara
authors.forename: Marina
authors.initials: M
authors.lastname: Iannello
authors.forename: Antonio L
authors.initials: AL
authors.lastname: De Lacey
authors.forename: Christine
authors.initials: C
authors.lastname: Cavazza
authors.forename: Juan Carlos
authors.initials: JC
authors.lastname: Fontecilla-Camps
mesh_terms: D020134:Catalytic Domain; D018360:Crystallography, X-Ray; D003901:Desulfovibrio / Q000201:enzymology*; D006859:Hydrogen / Q000737:chemistry; D006864:Hydrogenase / Q000737:chemistry / Q000378:metabolism*; D010084:Oxidation-Reduction; D010100:Oxygen / Q000737:chemistry*; D012643:Selenium / Q000737:chemistry; D013455:Sulfur / Q000737:chemistry
chemical_list: D013455:Sulfur; D006859:Hydrogen; C058560:nickel-iron-selenium hydrogenase; D006864:Hydrogenase; D012643:Selenium; D010100:Oxygen