title: MTRR A66G polymorphism among two caste groups of Uttar Pradesh (India).
abstract: OBJECTIVE
  This study was aimed to evaluate the Methionine synthase reductase (MTRR) A66G mutation in Yadav and Scheduled Caste (SC) population of Uttar Pradesh.
  
  
  METHODS
  Blood samples were collected from 100 subjects after taking informed written consent and PCR-RFLP method was used for the analysis of A66G mutation. After NdeI digestion, 66-bp amplicon of normal allele was cut into 22- and 44-bp long fragments, whereas mutant G allele was not digested.
  
  
  RESULTS
  Frequencies of genotypes in Yadav population AA, AG, and GG were 12%, 60%, and 28%, respectively, and in SC population, genotypic frequencies were 12% (AA), 52% (AG), and 36% (GG).
  
  
  CONCLUSIONS
  MTRR gene A66G mutation is found to be polymorphic in both the target populations with G allele frequencies being 0.58 for Yadav and 0.62 for Scheduled Caste.
authors.affiliation: Department of Biotechnology, Human Molecular Genetics Laboratory, VBS Purvanchal University, Jaunpur, Uttar Pradesh, India.
authors.forename: Vandana
authors.initials: V
authors.lastname: Rai
authors.forename: Upendra
authors.initials: U
authors.lastname: Yadav
authors.forename: Pradeep
authors.initials: P
authors.lastname: Kumar
mesh_terms: D005260:Female; D005287:Ferredoxin-NADP Reductase / Q000235:genetics*; D005838:Genotype; D006801:Humans; D007194:India; D008297:Male; D020641:Polymorphism, Single Nucleotide; D012923:Social Class
chemical_list: C111241:methionine synthase reductase; D005287:Ferredoxin-NADP Reductase