title: Analysis of the biological and molecular variability of the Polish isolates of Tomato black ring virus (TBRV).
abstract: Tomato black ring virus (TBRV) is an important pathogen infecting many plant species worldwide. The biological and molecular variability of the Polish isolates of TBRV was analyzed. The analysis was performed based on the symptoms induced by various isolates on test plant species as well as on phylogenetic relationships between isolates. Isolates differed in their host range and symptomatology. In addition, genetic variation among isolates was characterized by restriction fragment length polymorphism analysis and confirmed by sequencing. The phylogenetic analysis revealed that the Polish isolates differ from each other and do not form a monophyletic cluster. Finally, we identified and analyzed sequences of defective RNA forms arising from the TBRV genome.
authors.affiliation: Institute of Plant Protection-National Research Institute, ul. Wł. Węgorka 20, 60-318, Poznan, Poland.
authors.forename: N
authors.initials: N
authors.lastname: Rymelska
authors.forename: N
authors.initials: N
authors.lastname: Borodynko
authors.forename: H
authors.initials: H
authors.lastname: Pospieszny
authors.forename: B
authors.initials: B
authors.lastname: Hasiów-Jaroszewska
mesh_terms: D016000:Cluster Analysis; D014644:Genetic Variation; D058507:Host Specificity; D018551:Solanum lycopersicum / Q000821:virology*; D008969:Molecular Sequence Data; D017860:Nepovirus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology*; D011044:Poland; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012367:RNA, Viral / Q000235:genetics; D017422:Sequence Analysis, DNA; D017385:Sequence Homology
chemical_list: D012367:RNA, Viral