title: Toll-like receptor 9 (TLR9) gene polymorphisms associated with increased susceptibility of human papillomavirus-16 infection in patients with cervical cancer.
abstract: OBJECTIVE
  To investigate the association between toll-like receptor 9 (TLR9) single nucleotide polymorphisms (SNPs) and human papillomavirus (HPV) infection among Chinese Han women with cervical cancer.
  
  
  METHODS
  TLR9 -1486 and 2848 SNPs were investigated in patients with cervical cancer and controls using polymerase chain reaction (PCR)-restriction fragment length polymorphism. HPV16 E6 and E7 infections were assessed using PCR.
  
  
  RESULTS
  Of 120 patients with cervical cancer and 100 controls, there was a significant association between TLR9 2848 SNP and cervical cancer risk, but there was no such association with TLR9 -1486 SNP. Frequency of the TLR9 2848 GA genotype was significantly higher in patients with cervical cancer than in controls. There was no statistically significant between-group difference in presence of HPV16 infection. Presence of HPV infection with TLR9 2848 (rs352140) GA/AA genotype increased the risk of cervical cancer 13.8-fold compared with the GG genotype.
  
  
  CONCLUSIONS
  The TLR9 2848 G/A polymorphism in Chinese Han women was associated with increased risk of cervical cancer in the presence of HPV16 infection. Further studies are necessary to uncover the functional aspect of this TLR9 2848 polymorphism.
authors.affiliation: Department of Obstetrics and Gynaecology, West China Second Hospital, Sichuan University, Chengdu, China.
authors.forename: Zeng-Zhen
authors.initials: ZZ
authors.lastname: Lai
authors.lastname: Ni-Zhang
authors.forename: Xiao-Ling
authors.initials: XL
authors.lastname: Pan
authors.forename: Liang
authors.initials: L
authors.lastname: Song
mesh_terms: D000230:Adenocarcinoma / Q000175:diagnosis / Q000208:ethnology / Q000235:genetics* / Q000821:virology; D000328:Adult; D044466:Asian People; D002294:Carcinoma, Squamous Cell / Q000175:diagnosis / Q000208:ethnology / Q000235:genetics* / Q000821:virology; D016022:Case-Control Studies; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D052162:Human papillomavirus 16 / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D008875:Middle Aged; D009856:Oncogene Proteins, Viral / Q000235:genetics; D050725:Papillomavirus E7 Proteins / Q000235:genetics; D030361:Papillomavirus Infections / Q000175:diagnosis / Q000208:ethnology / Q000235:genetics* / Q000821:virology; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D012097:Repressor Proteins / Q000235:genetics; D012307:Risk Factors; D051217:Toll-Like Receptor 9 / Q000235:genetics*; D002583:Uterine Cervical Neoplasms / Q000175:diagnosis / Q000208:ethnology / Q000235:genetics* / Q000821:virology
chemical_list: C068651:E6 protein, Human papillomavirus type 16; D009856:Oncogene Proteins, Viral; D050725:Papillomavirus E7 Proteins; D012097:Repressor Proteins; C495371:TLR9 protein, human; D051217:Toll-Like Receptor 9; C059731:oncogene protein E7, Human papillomavirus type 16
keywords: Human papillomavirus (HPV); Toll-like receptor 9 (TLR9); cervical cancer; gene polymorphism; rs352140; single nucleotide polymorphism (SNP)