title: Species identification of Asini Corii Collas (donkey glue) by PCR amplification of cytochrome b gene.
abstract: Asini Corii Collas (ACC; donkey glue) is a crude drug used to promote hematopoiesis and arrest bleeding. Because adulteration of the drug with substances from other animals such as horses, cattle, and pigs has been found, we examined PCR methods based on the sequence of the cytochrome b gene for source species identification. Two strategies for extracting DNA from ACC were compared, and the ion-exchange resin procedure was revealed to be more suitable than the silica-based one. Using DNA extracted from ACC by the ion-exchange resin procedure, PCR methods for species-specific detection of donkey, horse, cattle, and pig substances were established. When these species-specific PCR methods were applied to ACC, amplicons were obtained only by the donkey-specific PCR. Cattle-specific PCR detected as little as 0.1% admixture of cattle glue in the ACC. These results suggest that the species-specific PCR methods established in this study would be useful for simple and easy detection of adulteration of ACC.
authors.affiliation: Division of Pharmacognosy, Phytochemistry and Narcotics, National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya-ku, Tokyo, 158-8501, Japan.
authors.forename: Yukie
authors.initials: Y
authors.lastname: Kumeta
authors.forename: Takuro
authors.initials: T
authors.lastname: Maruyama
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Asama
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Yamamoto
authors.forename: Takashi
authors.initials: T
authors.lastname: Hakamatsuka
authors.forename: Yukihiro
authors.initials: Y
authors.lastname: Goda
mesh_terms: D000269:Adhesives / Q000592:standards; D000818:Animals; D002417:Cattle; D002852:Chromatography, Ion Exchange; D045303:Cytochromes b / Q000235:genetics*; D004247:DNA / Q000032:analysis*; D058893:DNA Barcoding, Taxonomic; D004340:Drug Contamination; D018397:Equidae / Q000235:genetics* / Q000378:metabolism; D006736:Horses; D016133:Polymerase Chain Reaction / Q000379:methods; D011786:Quality Control; D013045:Species Specificity; D013552:Swine; D014020:Tissue Extracts / Q000235:genetics* / Q000378:metabolism / Q000592:standards
chemical_list: D000269:Adhesives; D014020:Tissue Extracts; D004247:DNA; D045303:Cytochromes b