title: Amorphous areas in the cytoplasm of Dendrobium tepal cells: production through organelle degradation and destruction through macroautophagy?
abstract: In Dendrobium flowers some tepal mesophyll cells showed cytoplasmic areas devoid of large organelles. Such amorphous areas comprised up to about 40% of the cross-section of a cell. The areas were not bound by a membrane. The origin of these areas is not known. We show data suggesting that they can be formed from vesicle-like organelles. The data imply that these organelles and other material become degraded inside the cytoplasm. This can be regarded as a form of autophagy. The amorphous areas became surrounded by small vacuoles, vesicles or double membranes. These seemed to merge and thereby sequester the areas. Degradation of the amorphous areas therefore seemed to involve macroautophagy.
authors.affiliation: Mann Laboratory; Department of Plant Sciences; University of California, Davis; Davis, CA USA.
authors.forename: Wouter G
authors.initials: WG
authors.lastname: van Doorn
authors.forename: Kanjana
authors.initials: K
authors.lastname: Kirasak
authors.forename: Saichol
authors.initials: S
authors.lastname: Ketsa
mesh_terms: D001343:Autophagy; D002467:Cell Nucleus / Q000648:ultrastructure; D031667:Dendrobium / Q000166:cytology* / Q000648:ultrastructure; D058503:Mesophyll Cells / Q000166:cytology / Q000648:ultrastructure; D015388:Organelles / Q000378:metabolism*; D014617:Vacuoles / Q000648:ultrastructure
keywords: amorphous area; autolysosome; autophagosome; cytoplasm; engulfment; membrane; mesophyll; plant; plastid; tepal; vacuole