title: Antimycobacterial activity of Citrullus colocynthis (L.) Schrad. against drug sensitive and drug resistant Mycobacterium tuberculosis and MOTT clinical isolates.
abstract: BACKGROUND
  Citrullus colocynthis (Cucurbitaceae), a folk herbal medicine and traditionally used natural remedy for tuberculosis in India has been studied to validate its antitubercular activity against drug sensitive and drug resistant (including multidrug resistant) Mycobacterium tuberculosis and Mycobacterium other than tuberculosis (MOTT) bacilli.
  
  
  METHODS
  Inhibitory and bactericidal activities of crude extracts, fractions and compounds of Citrullus colocynthis plant, consisting of aerial parts and ripe deseeded fruits were performed against the drug sensitive standard strain of Mycobacterium tuberculosis H37Rv (ATCC 27294), 16 drug resistant strains of Mycobacterium tuberculosis and two MOTT strains, using radiometric BACTEC 460TB system.
  
  
  RESULTS
  Methanolic extract of ripe deseeded fruit of Citrullus colocynthis has shown good activity (MIC ≤ 62.5 µg/ml), whereas among the bioactive fractions, FC IX demonstrated the best activity (MIC 31.2 µg/ml) against Mycobacterium tuberculosis H37Rv. Bioactive FC III, IX and X also inhibited 16 clinical isolates of Mycobacterium tuberculosis consisting of seven non-multidrug resistants, eight multidrug resistants, one extensively drug resistant and two of MOTTs with MICs in the range of 50-125, 31.2-125 and 62.5-125 µg/ml, respectively. Ursolic acid and cucurbitacin E 2-0-β-d-glucopyranoside were identified as the main biomarkers active against Mycobacterium tuberculosis H37Rv (MICs 50 and 25 µg/ml respectively), as well as against the 18 clinical isolates. FC III and FC IX showed better inhibition of drug resistant and MOTT clinical isolates. Minimal bactericidal concentrations of extracts, fractions and compound C-2 were ≥ two-fold MIC values.
  
  
  CONCLUSIONS
  The study provides a scientific rationale for the traditional use of Citrullus colocynthis fruit in the treatment of tuberculosis. In addition, the study elucidates a broad spectrum antimycobacterial action of Citrullus colocynthis fruit, which can contribute to the development of improved preparation of an antitubercular natural drug for the treatment of drug resistant tuberculosis and MOTT infection as well.
authors.affiliation: Department of Botany, University of Rajasthan, Jaipur 302004, India.
authors.forename: Archana
authors.initials: A
authors.lastname: Mehta
authors.forename: Garima
authors.initials: G
authors.lastname: Srivastva
authors.forename: Sumita
authors.initials: S
authors.lastname: Kachhwaha
authors.forename: Meenakshi
authors.initials: M
authors.lastname: Sharma
authors.forename: S L
authors.initials: SL
authors.lastname: Kothari
mesh_terms: D000995:Antitubercular Agents / Q000302:isolation & purification / Q000494:pharmacology*; D029773:Citrullus / Q000737:chemistry* / Q000254:growth & development; D020834:Ethnopharmacology; D005638:Fruit / Q000737:chemistry; D006801:Humans; D007194:India; D008826:Microbial Sensitivity Tests; D009161:Mycobacterium / Q000187:drug effects / Q000254:growth & development / Q000302:isolation & purification; D009169:Mycobacterium tuberculosis / Q000187:drug effects* / Q000254:growth & development / Q000302:isolation & purification; D035261:Plant Components, Aerial / Q000737:chemistry; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D018088:Tuberculosis, Multidrug-Resistant / Q000188:drug therapy / Q000382:microbiology*; D014397:Tuberculosis, Pulmonary / Q000188:drug therapy / Q000382:microbiology*
chemical_list: D000995:Antitubercular Agents; D010936:Plant Extracts
keywords: Citrullus colocynthis Cucurbitaceae; Drug resistant; Minimal bactericidal concentration.; Minimal inhibitory concentration; Mycobacterium other than tuberculosis (MOTT); Mycobacterium tuberculosis