title: Lack of detection of the mouse mammary tumor-like virus (MMTV) Env gene in Iranian women breast cancer using real time PCR.
abstract: BACKGROUND
  Mouse mammary tumor virus (MMTV) is the major cause of mammary tumors in mice. There is limited controversial evidence about the probable etiologic role of MMTV- like virus in human breast cancer.
  
  
  METHODS
  A total of 40 Formalin fixed paraffin embedded samples with diagnosis of breast cancer were collected in a period of 3 years from cancer institute of Iran. We selected both pre-menopausal and post-menopausal patients with different histologic grades and different ethnic groups. We evaluated presence of MMTV-like virus env gene through real time PCR method.
  
  
  RESULTS
  Forty patients (20 pre and 20 post- menopausal women) were evaluated with the mean age of 49.67. The average tumor size was 39 mm. None of the studied samples were positive for MMTV-like virus env gene target sequences.
  
  
  CONCLUSIONS
  We found no evidence on the potential role of MMTV-like virus in the carcinogenicity of breast cancer among Iranian women.
authors.affiliation: Pathology Department, Sina Hospital, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Hedieh Moradi
authors.initials: HM
authors.lastname: Tabriz
authors.forename: Kazem
authors.initials: K
authors.lastname: Zendehdel
authors.forename: Reza
authors.initials: R
authors.lastname: Shahsiah
authors.forename: Forouzandeh
authors.initials: F
authors.lastname: Fereidooni
authors.forename: Baharak
authors.initials: B
authors.lastname: Mehdipour
authors.forename: Zahra Mostakhdemin
authors.initials: ZM
authors.lastname: Hosseini
mesh_terms: D001943:Breast Neoplasms / Q000821:virology*; D044584:Carcinoma, Ductal / Q000821:virology*; D004279:DNA, Viral / Q000235:genetics*; D005260:Female; D005557:Formaldehyde; D015686:Gene Products, env / Q000235:genetics*; D006801:Humans; D007492:Iran; D008324:Mammary Tumor Virus, Mouse / Q000235:genetics*; D008875:Middle Aged; D016612:Paraffin Embedding; D016133:Polymerase Chain Reaction
chemical_list: D004279:DNA, Viral; D015686:Gene Products, env; D005557:Formaldehyde