title: Physicochemical and biological characterization of nanocomposites made of segmented polyurethanes and Cloisite 30B.
abstract: Nanocomposites were prepared with segmented polyurethanes and Cloisite 30B by either solution mixing or in situ polymerization and characterized by Fourier transform infrared spectroscopy, differential scanning calorimetry, thermogravimetric analysis and X-ray diffraction. Cytotoxicity and genotoxicity were assessed with lymphocytes while cell viability was measured by the methyl tetrazolium assay using fibroblasts. It was found that in situ polymerization rendered exfoliated nanocomposites with higher glass transition temperature, tensile modulus and thermal stability compared to nanocomposites obtained by solution mixing. The mitotic index of lymphocytes was significantly reduced at high clay concentrations (6 wt% and 10 wt%), while fibroblast viability improved in the presence of extract obtained after days 2 and 7.
authors.affiliation: Centro de Investigación Científica de Yucatán A.C., México.
authors.forename: J I
authors.initials: JI
authors.lastname: Moo-Espinosa
authors.forename: R
authors.initials: R
authors.lastname: Solís-Correa
authors.forename: R
authors.initials: R
authors.lastname: Vargas-Coronado
authors.forename: J M
authors.initials: JM
authors.lastname: Cervantes-Uc
authors.forename: J V
authors.initials: JV
authors.lastname: Cauich-Rodríguez
authors.forename: P Quintana
authors.initials: PQ
authors.lastname: Owen
authors.forename: M A
authors.initials: MA
authors.lastname: Aguilar-Santamaría
authors.forename: Mar Fernández
authors.initials: MF
authors.lastname: Gutiérrez
authors.forename: J San Román
authors.initials: JS
authors.lastname: del Barrio
mesh_terms: D001546:Bentonite / Q000737:chemistry* / Q000633:toxicity; D001672:Biocompatible Materials / Q000737:chemistry* / Q000633:toxicity; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects; D006801:Humans; D008214:Lymphocytes / Q000166:cytology / Q000187:drug effects; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D008938:Mitosis / Q000187:drug effects; D053761:Nanocomposites / Q000737:chemistry* / Q000633:toxicity / Q000648:ultrastructure; D036103:Nanotechnology; D011140:Polyurethanes / Q000737:chemistry* / Q000633:toxicity; D017550:Spectroscopy, Fourier Transform Infrared; D013718:Tensile Strength; D013816:Thermodynamics; D014961:X-Ray Diffraction
chemical_list: D001672:Biocompatible Materials; D011140:Polyurethanes; C115016:polyetherurethane; D001546:Bentonite
keywords: Segmented polyurethanes; cytotoxicity; genotoxicity; montmorillonite; nanocomposites