title: S100B and NSE as useful postmortem biochemical markers of traumatic brain injury in autopsy cases.
abstract: Postmortem analysis of relevant biomarkers might aid in characterizing causes of death and survival times in legal medicine. However, there are still no sufficiently established results of practical postmortem biochemical investigations in cases of traumatic brain injury (TBI). The two biomarkers--S100 protein subunit B (S100B) and neuronal specific enolase (NSE)--could be of special interest. Therefore, the aim of the present study was to investigate changes in their postmortem levels for further determination of brain damage in TBI. In 17 cases of TBI (average age, 58 years) and in 23 controls with different causes of death (average age, 59 years), serum and cerebrospinal fluid (CSF) samples were analyzed with a chemiluminescence immunoassay for marker expression. An increase in serum S100B, as well as a subsequent decrease after survival times>4 days, were detected in TBI cases (p<0.01). CSF NSE values >6,000 ng/mL and CSF S100B levels >10,000 ng/mL seem to indicate a TBI survival time of at least 15 min (p<0.01). It is of particular interest that CSF S100B levels (p<0.01) and serum S100B levels (p<0.05) as well as CSF NSE values (p<0.01) were significantly higher in TBI cases in comparison to the controls, especially when compared with fatal non-head injuries. In conclusion, the present findings emphasize that S100B and NSE are useful markers in postmortem biochemistry in cases of suspected TBI. Further, S100B may be helpful to estimate the survival time of fatal injuries in legal medicine.
authors.affiliation: 1 Institute of Legal Medicine, Medical Faculty University of Leipzig, Leipzig , Germany .
authors.forename: Benjamin
authors.initials: B
authors.lastname: Ondruschka
authors.forename: Dirk
authors.initials: D
authors.lastname: Pohlers
authors.forename: Gerald
authors.initials: G
authors.lastname: Sommer
authors.forename: Kristin
authors.initials: K
authors.lastname: Schober
authors.forename: Daniel
authors.initials: D
authors.lastname: Teupser
authors.forename: Heike
authors.initials: H
authors.lastname: Franke
authors.forename: Jan
authors.initials: J
authors.lastname: Dressler
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001344:Autopsy; D015415:Biomarkers; D001930:Brain Injuries / Q000097:blood* / Q000134:cerebrospinal fluid* / Q000473:pathology; D002423:Cause of Death; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D010751:Phosphopyruvate Hydratase / Q000097:blood* / Q000134:cerebrospinal fluid*; D064568:S100 Calcium Binding Protein beta Subunit / Q000097:blood* / Q000134:cerebrospinal fluid*; D016019:Survival Analysis; D013997:Time Factors; D055815:Young Adult
chemical_list: D015415:Biomarkers; D064568:S100 Calcium Binding Protein beta Subunit; C579160:S100B protein, human; D010751:Phosphopyruvate Hydratase