title: Population dynamics and in vitro antibody pressure of porcine parvovirus indicate a decrease in variability.
abstract: To estimate the impact of porcine parvovirus (PPV) vaccines on the emergence of new phenotypes, the population dynamic history of the virus was calculated using the Bayesian Markov chain Monte Carlo method with a Bayesian skyline coalescent model. Additionally, an in vitro model was performed with consecutive passages of the 'Challenge' strain (a virulent field strain) and NADL2 strain (a vaccine strain) in a PK-15 cell line supplemented with polyclonal antibodies raised against the vaccine strain. A decrease in genetic diversity was observed in the presence of antibodies in vitro or after vaccination (as estimated by the in silico model). We hypothesized that the antibodies induced a selective pressure that may reduce the incidence of neutral selection, which should play a major role in the emergence of new mutations. In this scenario, vaccine failures and non-vaccinated populations (e.g. wild boars) may have an important impact in the emergence of new phenotypes.
authors.affiliation: CAPES Foundation, Ministry of Education of Brazil, Setor Bancário Norte, Quadra 2, Bloco L, Lote 06, 70040-020, Brasília, Brazil.|Institute of Animal Hygiene and Veterinary Public Health, Faculty of Veterinary Medicine, University of Leipzig, An den Tierkliniken 1, 04103, Leipzig, Germany.
authors.forename: André Felipe
authors.initials: AF
authors.lastname: Streck
authors.affiliation: Institute of Animal Hygiene and Veterinary Public Health, Faculty of Veterinary Medicine, University of Leipzig, An den Tierkliniken 1, 04103, Leipzig, Germany.
authors.forename: Timo
authors.initials: T
authors.lastname: Homeier
authors.affiliation: Institute of Animal Hygiene and Veterinary Public Health, Faculty of Veterinary Medicine, University of Leipzig, An den Tierkliniken 1, 04103, Leipzig, Germany.
authors.forename: Tessa
authors.initials: T
authors.lastname: Foerster
authors.affiliation: Institute of Animal Hygiene and Veterinary Public Health, Faculty of Veterinary Medicine, University of Leipzig, An den Tierkliniken 1, 04103, Leipzig, Germany.
authors.forename: Uwe
authors.initials: U
authors.lastname: Truyen
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D014644:Genetic Variation; D015233:Models, Statistical; D008969:Molecular Sequence Data; D028323:Parvovirus, Porcine / Q000145:classification* / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification; D011157:Population Dynamics; D012641:Selection, Genetic; D017422:Sequence Analysis, DNA; D013552:Swine
chemical_list: D000914:Antibodies, Viral; D004279:DNA, Viral