title: Recombinant Newcastle disease virus Anhinga strain (NDV/Anh-EGFP) for hepatoma therapy.
abstract: Hepatocellular carcinoma remains one of the most common malignant tumors in the world. Newcastle disease virus (NDV) has been proved to be an efficient oncolytic agent. NDV tumor killing efficacy is not only dependening on the NDV strain but the type of tumor targeted. It is significant to discover more effective and safe oncolytic strains. We investigated the effectiveness of genetically engineered NDV Anhinga strain in hepatoma treatment. The modified virus containing an insertion of enhanced green fluorescent protein (EGFP), named NDV/Anh-EGFP. The antitumor efficacy of the recombinant virus on hepatoma was examined both in vivo and in vitro. NDV Anhinga strain, which could be classified as a lytic strain, is an effective oncolytic agent on hepatoma. There was no significant difference in the TCID50 and growth capability between the recombinant NDV and the parental. NDV/Anh-EGFP can obviously inhibit hepatocarcinoma development in vitro and in vivo. We demonstrate Anhinga strain could become a potent candidate for clinical carcinoma therapy especially for hepatocarcinoma.
authors.affiliation: College of Life Science, Northeast Agriculture University, Mucai Street 59, Xiangfang District, Harbin, China. deshanli@163.com.
authors.forename: Yunzhou
authors.initials: Y
authors.lastname: Wu
authors.forename: Shijun
authors.initials: S
authors.lastname: Yan
authors.forename: Zheng
authors.initials: Z
authors.lastname: Lv
authors.forename: Lin
authors.initials: L
authors.lastname: Chen
authors.forename: Jingshu
authors.initials: J
authors.lastname: Geng
authors.forename: Jinjiao
authors.initials: J
authors.lastname: He
authors.forename: Qingzhong
authors.initials: Q
authors.lastname: Yu
authors.forename: Jiechao
authors.initials: J
authors.lastname: Yin
authors.forename: Guiping
authors.initials: G
authors.lastname: Ren
authors.forename: Deshan
authors.initials: D
authors.lastname: Li
mesh_terms: D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000473:pathology / Q000628:therapy; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D004195:Disease Models, Animal; D015870:Gene Expression; D023061:Gene Order; D017930:Genes, Reporter; D005822:Genetic Vectors / Q000235:genetics*; D056945:Hep G2 Cells; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics* / Q000473:pathology / Q000628:therapy; D009522:Newcastle disease virus / Q000235:genetics*; D014161:Transduction, Genetic; D047368:Tumor Burden; D014779:Virus Replication; D023041:Xenograft Model Antitumor Assays