title: Inhibition of histone deacetylation alters Arabidopsis root growth in response to auxin via PIN1 degradation.
abstract: Our results showed the histone deacetylase inhibitors (HDIs) control root development in Arabidopsis via regulation of PIN1 degradation. Epigenetic regulation plays a crucial role in the expression of many genes in response to exogenous or endogenous signals in plants as well as other organisms. One of epigenetic mechanisms is modifications of histone, such as acetylation and deacetylation, are catalyzed by histone acetyltransferase (HAT) and histone deacetylase (HDAC), respectively. The Arabidopsis HDACs, HDA6, and HDA19, were reported to function in physiological processes, including embryo development, abiotic stress response, and flowering. In this study, we demonstrated that histone deacetylase inhibitors (HDIs) inhibit primary root elongation and lateral root emergence. In response to HDIs treatment, the PIN1 protein was almost abolished in the root tip. However, the PIN1 gene did not show decreased expression in the presence of HDIs, whereas IAA genes exhibited increases in transcript levels. In contrast, we observed a stable level of gene expression of stress markers (KIN1 and COR15A) and a cell division marker (CYCB1). Taken together, these results suggest that epigenetic regulation may control auxin-mediated root development through the 26S proteasome-mediated degradation of PIN1 protein.
authors.affiliation: Department of Biosystems and Biotechnology, College of Life Sciences and Biotechnology, Korea University, 1, 5-ka Anam-dong, Seongbuk-ku, Seoul, 136-713, Republic of Korea.
authors.forename: Hoai Nguyen
authors.initials: HN
authors.lastname: Nguyen
authors.forename: Jun Hyeok
authors.initials: JH
authors.lastname: Kim
authors.forename: Chan Young
authors.initials: CY
authors.lastname: Jeong
authors.forename: Suk-Whan
authors.initials: SW
authors.lastname: Hong
authors.forename: Hojoung
authors.initials: H
authors.lastname: Lee
mesh_terms: D017360:Arabidopsis / Q000235:genetics* / Q000254:growth & development; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D044127:Epigenesis, Genetic; D018506:Gene Expression Regulation, Plant; D056572:Histone Deacetylase Inhibitors / Q000494:pharmacology*; D007210:Indoleacetic Acids / Q000378:metabolism; D026901:Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism*; D018517:Plant Roots / Q000235:genetics / Q000254:growth & development*; D030821:Plants, Genetically Modified / Q000235:genetics / Q000254:growth & development; D046988:Proteasome Endopeptidase Complex / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; D056572:Histone Deacetylase Inhibitors; D007210:Indoleacetic Acids; D026901:Membrane Transport Proteins; C116363:PIN1 protein, Arabidopsis; D046988:Proteasome Endopeptidase Complex; C061553:ATP dependent 26S protease