title: Identification and expression profiles of IL-8 in bighead carp (Aristichthys nobilis) in response to microcystin-LR.
abstract: Microcystin-LR (MCLR) is a widespread cyanotoxin and has immunotoxicity to animals, including fish. Chemokines are considered to play important roles in inflammatory response induced by MCLR. In this study, we cloned the full-length cDNA of interleukin-8 (IL-8) from bighead carp (Aristichthys nobilis) for the first time. The full-length IL-8 cDNA was 552 bp and contained a 297-bp open-reading frame that encoded for a 98-amino acid protein. The deduced IL-8 protein had a typical aspartic acid (D)-leucine (L)-arginine (R) and a CXC motif at the N-terminal, which were conserved in most fish species. Phylogenetic analysis showed that bighead carp IL-8 protein was grouped in the teleost IL-8 lineage 2. Under normal conditions, the expression of IL-8 is constitutive and weak in all tested tissues. However, MCLR treatment could significantly increase the transcription of IL-8 in bighead carp in a temporal- and dose-dependent pattern. The present study will help us to understand more about the evolution of IL-8 and its function in the MCLR induced proinflammatory response in bighead carp.
authors.affiliation: Donghu Experimental Station of Lake Ecosystems, State Key Laboratory of Freshwater Ecology and Biotechnology of China, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, People's Republic of China.
authors.forename: Huiying
authors.initials: H
authors.lastname: Li
authors.forename: Yan
authors.initials: Y
authors.lastname: Cai
authors.forename: Ping
authors.initials: P
authors.lastname: Xie
authors.forename: Guangyu
authors.initials: G
authors.lastname: Li
authors.forename: Le
authors.initials: L
authors.lastname: Hao
authors.forename: Qian
authors.initials: Q
authors.lastname: Xiong
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002347:Carps / Q000235:genetics* / Q000378:metabolism; D002681:China; D018076:DNA, Complementary / Q000235:genetics / Q000378:metabolism; D004781:Environmental Exposure; D029941:Fish Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D016209:Interleukin-8 / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D060106:Lakes; D008387:Marine Toxins; D052998:Microcystins / Q000633:toxicity*; D008969:Molecular Sequence Data; D021141:Nucleic Acid Amplification Techniques; D010802:Phylogeny; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D016415:Sequence Alignment; D014018:Tissue Distribution; D059467:Transcriptome / Q000187:drug effects*
chemical_list: D018076:DNA, Complementary; D029941:Fish Proteins; D016209:Interleukin-8; D008387:Marine Toxins; D052998:Microcystins; D012333:RNA, Messenger; C057862:cyanoginosin LR