title: [Mitochondrial mechanisms of apoptosis of human leukemia K562 cells induced by AVVC-1].
abstract: This study was purpose to investigate apoptosis pathway of leukemia K562 cells induced by anticoagulant fraction from Agkistrodon acutus venom (AVVC-1). The mitochondrial transmembrane potential (ΔΨm) of leukemia K562 cells was detected by flow cytometry with JC-1 single staining. The expression of cytochrome C in the mitochondrial of leukemia K562 cells was analyzed by Western blot after AVVC-1 treatment. The distribution of cytochrome C in leukemia K562 cells was measured by immuno-fluorescence test. The results showed that the potential of mitochondrial membrane decreased after treatment with different concentrations of AVVC-1 (12.5, 25, 50, 100 µg/ml) for 6 h (P < 0.01). The expression level of cytochrome C protein in mitochondria obviously declined after treatment with 30 µg/ml AVVC-1 for 48 h, and the fluorescent intensity of cytochrome C in cytosol was enhanced at the same time. It is concluded that AVVC-1-induced K562 cell apoptosis is related with mitochondrial damage, and cytochrome C may be a useful agent for investigating human leukemia therapy by using AVVC-1.
authors.affiliation: Department of Pathophysioloy, Wannan Medical College, Wuhu, Anhui Province, China.
authors.forename: Ru-Qi
authors.initials: RQ
authors.lastname: Zheng
authors.forename: Gen-Bao
authors.initials: GB
authors.lastname: Zhang
authors.forename: Lu
authors.initials: L
authors.lastname: Huang
authors.forename: Kai-Ran
authors.initials: KR
authors.lastname: Ma
authors.forename: Juan
authors.initials: J
authors.lastname: Wu
authors.forename: Shu
authors.initials: S
authors.lastname: Li
mesh_terms: D017836:Agkistrodon; D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D045304:Cytochromes c / Q000378:metabolism; D006801:Humans; D020014:K562 Cells; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects*; D008928:Mitochondria / Q000378:metabolism*; D012910:Snake Venoms / Q000494:pharmacology*
chemical_list: D012910:Snake Venoms; D045304:Cytochromes c