title: Aptamer--nanoparticle-based chemiluminescence for p53 protein.
abstract: A simple colorimetric biosensing technique based on the interaction of gold nanoparticles (AuNPs) with the aptamer was developed for detection of p53, a tumor suppressor protein, in the current study. Aggregation of AuNPs was induced by desorption of the p53 binding RNA aptamer from the surface of AuNPs as a result of the aptamer target interaction leading to the color change of AuNPs from red to purple. The detection limit of p53 protein by the colorimetric approach was 0.1 ng/ml after successful optimization of the amount of aptamer, AuNPs, salts, and incubation time. Furthermore, the catalytic activity of the aggregated AuNPs was greatly enhanced by chemiluminescence (CL) reaction, where the detection limit was enhanced to 10 pg/ml with a regression coefficient of R2 = 0.9907. Here the sensitivity was increased by 10-fold compared with the AuNP-based colorimetric method. Hence, the sensitivity of detection was increased by employing CL, by using the catalytic activity of aggregated AuNPs, on the luminol-hydrogen peroxide reaction. Thus, the combination of colorimetric and CL-based aptasensor can be of great advantage in increasing the sensitivity of detection for any target analyte.
authors.affiliation: Fermentation Technology and Bioengineering Department, CSIR-Central Food Technological Research Institute, Mysore, Karnataka 570 020, India.
authors.forename: N
authors.initials: N
authors.lastname: Shwetha
authors.forename: L S
authors.initials: LS
authors.lastname: Selvakumar
authors.forename: M S
authors.initials: MS
authors.lastname: Thakur
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry*; D015374:Biosensing Techniques / Q000379:methods*; D003124:Colorimetry / Q000379:methods*; D006046:Gold / Q000737:chemistry; D006801:Humans; D006861:Hydrogen Peroxide / Q000737:chemistry; D049449:Luminescence; D008163:Luminescent Measurements / Q000379:methods*; D008165:Luminol / Q000737:chemistry; D053768:Metal Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D012965:Sodium Chloride / Q000737:chemistry; D013499:Surface Properties; D016159:Tumor Suppressor Protein p53 / Q000032:analysis*
chemical_list: D052157:Aptamers, Nucleotide; D016159:Tumor Suppressor Protein p53; D012965:Sodium Chloride; D008165:Luminol; D006046:Gold; D006861:Hydrogen Peroxide
keywords: 2′-O-methyl modified RNA aptamer; Aptasensor; AuNPs; Chemiluminescence; Nanosensor; p53 Protein