title: [Regulation of key enzymes of L-alanine biosynthesis by Brevibacterium flavum producer strains].
abstract: The mechanisms of L-alanine overproduction by Brevibacterium flavum producer strains were studied. It was shown that beta-CI-L-alanine is an inhibitor of some key enzymes involved in the synthesis of L-alanine, including alanine transaminase and valine-pyruvate transaminase. Two highly active B. flavum GL1 and GL1 8 producer strains, which are resistant to the inhibitory effect of beta-Cl-L-alanine, were obtained using a parental B. flavum AA5 producer strain, characterized by a reduced activity of alanine racemase (>or=98%). It was demonstrated that the increased L-alanine synthesis efficiency observed in the producer strains developed in this work is associated with the absence of inhibition of alanine transaminase by the end product of the biosynthesis reaction, as well as with the effect of derepression of both alanine transaminase and valine-pyruvate transaminase synthesis by the studied compound.
authors.forename: L O
authors.initials: LO
authors.lastname: Melkonian
authors.forename: G E
authors.initials: GE
authors.lastname: Avetisova
authors.forename: A A
authors.initials: AA
authors.lastname: Ambartsumian
authors.forename: A Kh
authors.initials: AKh
authors.lastname: Chakhalian
authors.forename: A S
authors.initials: AS
authors.lastname: Sagian
mesh_terms: D000409:Alanine / Q000378:metabolism; D000411:Alanine Racemase / Q000378:metabolism*; D000410:Alanine Transaminase / Q000378:metabolism*; D000613:Aminobutyrates / Q000378:metabolism; D001426:Bacterial Proteins / Q000378:metabolism*; D040142:Brevibacterium flavum / Q000187:drug effects / Q000201:enzymology*; D003523:Cycloserine / Q000494:pharmacology; D057075:Enzyme Assays; D007700:Kinetics; D000637:Transaminases / Q000378:metabolism*; D014633:Valine / Q000378:metabolism; D015091:beta-Alanine / Q000031:analogs & derivatives / Q000494:pharmacology
chemical_list: D000613:Aminobutyrates; D001426:Bacterial Proteins; D015091:beta-Alanine; C006977:3-chloroalanine; D003523:Cycloserine; D000637:Transaminases; D000410:Alanine Transaminase; C034522:valine-pyruvate transaminase; D000411:Alanine Racemase; D014633:Valine; D000409:Alanine