title: Solubilized matrix derived from decellularized liver as a growth factor-immobilizable scaffold for hepatocyte culture.
abstract: Tissue engineering requires growth factors, cells and a scaffold to permit effective tissue regeneration. This study focused on the development of a scaffold for liver tissue engineering, because the liver is a central organ for metabolism. We aimed to develop a scaffold to promote expression of liver-specific functions of hepatocytes, with a focus on immobilizing growth factors onto an organ-specific matrix for liver tissue regeneration. Solubilized extracellular matrix from decellularized liver (L-ECM) was obtained following Triton X-100 treatment and consisted of protein and polysaccharide. L-ECM was found to immobilize hepatocyte growth factor (HGF), even in the presence of albumin, with an efficiency of 75%. Additionally, the immobilized HGF on L-ECM film was stably remained in culture condition for 5 days. Immobilized HGF promoted hepatocyte migration, thus indicating that L-ECM-immobilized HGF maintained its native biological activity. Furthermore, L-ECM stimulated the expression of liver-specific functions, including albumin secretion, urea synthesis and ethoxyresorufin-O-deethylase activity, in primary rat hepatocytes cultured in growth factor-free medium. In summary, L-ECM has the potential to become an effective material in the field of regenerative medicine.
authors.affiliation: Department of Chemical Engineering, Faculty of Engineering, Graduate School, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan.
authors.forename: Shintaro
authors.initials: S
authors.lastname: Nakamura
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Ijima
mesh_terms: D000818:Animals; D005109:Extracellular Matrix / Q000378:metabolism*; D017228:Hepatocyte Growth Factor / Q000378:metabolism*; D022781:Hepatocytes / Q000166:cytology* / Q000378:metabolism; D055767:Immobilized Proteins / Q000378:metabolism; D008115:Liver Regeneration; D017830:Octoxynol / Q000378:metabolism; D061251:Primary Cell Culture; D051381:Rats; D012995:Solubility; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds
chemical_list: D055767:Immobilized Proteins; D017228:Hepatocyte Growth Factor; D017830:Octoxynol
keywords: Decellularized liver; Extracellular matrix; Growth factor; Hepatocyte; Immobilization