title: Characterization of Tn5801.Sag, a variant of Staphylococcus aureus Tn916 family transposon Tn5801 that is widespread in clinical isolates of Streptococcus agalactiae.
abstract: Tn5801, originally detected in Staphylococcus aureus Mu50, is a Tn916 family element in which a unique int gene (int5801) replaces the int and xis genes in Tn916 (int916 and xis916). Among 62 tet(M)-positive tetracycline-resistant Streptococcus agalactiae isolates, 43 harbored Tn916, whereas 19 harbored a Tn5801-like element (Tn5801.Sag, ∼20.6 kb). Tn5801.Sag was characterized (PCR mapping, partial sequencing, and chromosomal integration) and compared to other Tn5801-like elements. Similar to Tn5801 from S. aureus Mu50, tested in parallel, Tn5801.Sag was unable to undergo circularization and conjugal transfer.
authors.affiliation: Unit of Microbiology, Departments of Biomedical Sciences and Public Health, Polytechnic University of Marche, Ancona, Italy.
authors.forename: Marina
authors.initials: M
authors.lastname: Mingoia
authors.forename: Eleonora
authors.initials: E
authors.lastname: Morici
authors.forename: Emily
authors.initials: E
authors.lastname: Tili
authors.forename: Eleonora
authors.initials: E
authors.lastname: Giovanetti
authors.forename: Maria Pia
authors.initials: MP
authors.lastname: Montanari
authors.forename: Pietro E
authors.initials: PE
authors.lastname: Varaldo
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D004251:DNA Transposable Elements; D006801:Humans; D007558:Italy; D013203:Staphylococcal Infections / Q000188:drug therapy / Q000382:microbiology; D013211:Staphylococcus aureus / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D013292:Streptococcus agalactiae / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D013752:Tetracycline / Q000494:pharmacology; D013753:Tetracycline Resistance / Q000187:drug effects / Q000235:genetics*
chemical_list: D000900:Anti-Bacterial Agents; D004251:DNA Transposable Elements; D013752:Tetracycline