title: Integrative analysis of prostate cancer aggressiveness.
abstract: BACKGROUND
  Clinical management of prostate cancer (PC) is still highly demanding on the identification of robust biomarkers which will allow a more precise prediction of disease progression.
  
  
  METHODS
  We profiled both mRNA expression and DNA copy number alterations (CNAs) from laser capture microdissected cells from 31 PC patients and 17 patients with benign prostatic hyperplasia using Affymetrix GeneChip® technology. PC patients were subdivided into an aggressive (Gleason Score 8 or higher, and/or T3/T4 and/or N+/M+) and non-aggressive (all others) form of PC. Furthermore, we correlated the two datasets, as genes whose varied expression is due to a chromosomal alteration, may suggest a causal implication of these genes in the disease. All statistical analyses were performed in R version 2.15.0 and Bioconductor version 1.8.1., respectively.
  
  
  RESULTS
  We confirmed several common altered chromosomal regions as well as recently discovered loci such as deletions on chromosomes 3p14.1-3p13 and 13q13.3-13q14.11 supporting a possible role for RYBP, RGC32, and ELF1 in tumor suppression. Integrative analysis of expression and CN data combined with data retrieved from online databases propose PTP4A3 and ELF1 as possible factors for tumor progression.
  
  
  CONCLUSIONS
  Copy number data analysis revealed some significant differences between aggressive and non-aggressive tumors, while gene expression data alone could not define an aggressive group of patients. The assessment of CNA may have diagnostic and prognostic value in PC.
authors.affiliation: Department of Medicine I, Division: Institute of Cancer Research, Medical University of Vienna, Vienna, Austria.
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Feik
authors.forename: Norbert
authors.initials: N
authors.lastname: Schweifer
authors.forename: Andreas
authors.initials: A
authors.lastname: Baierl
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Sommergruber
authors.forename: Christian
authors.initials: C
authors.lastname: Haslinger
authors.forename: Philipp
authors.initials: P
authors.lastname: Hofer
authors.forename: Agnes
authors.initials: A
authors.lastname: Maj-Hes
authors.forename: Stephan
authors.initials: S
authors.lastname: Madersbacher
authors.forename: Andrea
authors.initials: A
authors.lastname: Gsur
mesh_terms: D000328:Adult; D000368:Aged; D056915:DNA Copy Number Variations; D018450:Disease Progression; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D061346:Laser Capture Microdissection; D008297:Male; D008875:Middle Aged; D060787:Neoplasm Grading; D011467:Prostate / Q000378:metabolism / Q000473:pathology*; D011471:Prostatic Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D012333:RNA, Messenger
chemical_list: D012333:RNA, Messenger
keywords: biomarker; copy number alteration; gene expression; prostate cancer outcome