title: Motion-contrast laser speckle imaging of microcirculation within tissue beds in vivo.
abstract: Laser speckle imaging is widely used to monitor functional blood perfusion within tissue beds in vivo but traditionally has difficulty visualizing small blood vessels even when the exposure time of the detector is long. We report a simple method that uses the motion contrast of dynamic speckle patterns to noninvasively visualize the distribution of blood flow within tissue beds in vivo. We experimentally demonstrate that the motion contrast can significantly suppress the effect of static scattering, leading to enhanced visibility of the functional blood vessels, including capillaries when compared to the traditional laser speckle contrast imaging.
authors.affiliation: University of Washington, Department of Bioengineering, Seattle, Washington 98195, China.
authors.forename: Rong
authors.initials: R
authors.lastname: Liu
authors.forename: Jia
authors.initials: J
authors.lastname: Qin
authors.forename: Ruikang K
authors.initials: RK
authors.lastname: Wang
mesh_terms: D000818:Animals; D001783:Blood Flow Velocity / Q000502:physiology*; D002138:Calibration; D002196:Capillaries / Q000473:pathology; D002560:Cerebrovascular Circulation / Q000502:physiology*; D003287:Contrast Media; D003952:Diagnostic Imaging / Q000379:methods*; D004423:Ear / Q000098:blood supply; D004867:Equipment Design; D006439:Hemodynamics; D007834:Lasers; D051379:Mice; D008833:Microcirculation / Q000502:physiology*; D009038:Motion; D012542:Scattering, Radiation; D013997:Time Factors
chemical_list: D003287:Contrast Media