title: New method for the determination of the half inhibition concentration (IC50) of cholinesterase inhibitors.
abstract: A new and simple analytical method is described for the determination of the IC50 values of the inhibitors of the hydrolysis of acetylcholine (ACh) or acetylthiocholine (ATCh) by cholinesterases. The method is based on monitoring the time course of the pH value during the uninhibited and inhibited reaction. It requires only a pH meter with a suitable pH measuring cell and a small thermostated stirred batch reactor. The method has been validated for twelve different types of cholinesterase inhibitors. The determined IC50 values are comparable to those obtained by independent, more complicated, and expensive methods (Ellman's and pH-stat).
authors.affiliation: Department of Physical Chemistry, Faculty of Chemical Technology, University of Pardubice, Studentská 573, 532 10 Pardubice, Czech Republic.
authors.forename: Markéta
authors.initials: M
authors.lastname: Kovárová
authors.forename: Karel
authors.initials: K
authors.lastname: Komers
authors.forename: Sárka
authors.initials: S
authors.lastname: Stepánková
authors.forename: Patrik
authors.initials: P
authors.lastname: Parík
authors.forename: Alexander
authors.initials: A
authors.lastname: Cegan
mesh_terms: D002800:Cholinesterase Inhibitors / Q000494:pharmacology*; D006863:Hydrogen-Ion Concentration; D020128:Inhibitory Concentration 50
chemical_list: D002800:Cholinesterase Inhibitors