title: Measuring T cell function in innate immune models.
abstract: Innate immune responses often result in the activation and modulation of T lymphocyte function. Analysis of T lymphocytes in mouse models of innate immunity can allow understanding of the links between the innate and adaptive immune systems. Other T lymphocyte populations display innate-like functions. Isolation of T cells and evaluation of their surface proteins can provide data on T cell activation, as can an analysis of T cell proliferation. Further insight may be obtained by examining cytokine production via intracellular cytokine staining or ELISPOT to determine T cell function. This chapter describes methods for T cell isolation, measurement of surface protein expression, T cell proliferation, intracellular cytokine staining, and ELISPOT.
authors.affiliation: Biology Department, Drew University, Madison, NJ, USA.
authors.forename: Brianne R
authors.initials: BR
authors.lastname: Barker
mesh_terms: D056704:Adaptive Immunity; D000818:Animals; D049109:Cell Proliferation; D002469:Cell Separation / Q000379:methods*; D007113:Immunity, Innate; D051379:Mice; D011506:Proteins / Q000378:metabolism; D013499:Surface Properties; D013601:T-Lymphocytes / Q000166:cytology* / Q000276:immunology
chemical_list: D011506:Proteins