title: Chemical structures of the secondary cell wall polymers (SCWPs) isolated from bovine mastitis Streptococcus uberis.
abstract: The cell envelope of Gram-positive bacteria is decorated with a variety of polysaccharides. In this study wall teichoic acid (WTA) and neutral polysaccharides were isolated from the cell envelope of bovine mastitis Streptococcus uberis. The polysaccharides were released by lysozyme treatment, and purified by hydrophobic interaction chromatography. Further separation was achieved utilizing anion-exchange chromatography which yielded two products, that is, a neutral polysaccharide with a high content of Rha and less Glc (rhamnan) and an anionic phosphate-rich one containing glycerol and Glc (WTA). The structures of these molecules were elucidated applying 1D and 2D nuclear magnetic resonance experiments as well as chemical analyses. In the rhamnan sample two independent molecules were identified, that is, a glucorhamnan with the structure →2)-α-L-Rhap-(1→3)-[α-D-Glcp-(1→2)-]α-L-Rhap-(1→, and a homopolymeric rhamnan →2)-α-L-Rhap-(1→3)-α-L-Rhap-(1→. The WTA comprised a polyphosphoglycerol chain substituted nonstoichiometrically with β-Glcp.
authors.affiliation: Division of Structural Biochemistry, Research Center Borstel, Leibniz-Center for Medicine and Biosciences, Airway Research Center North (ARCN), Borstel, Germany.
authors.forename: Anna
authors.initials: A
authors.lastname: Czabańska
authors.forename: Otto
authors.initials: O
authors.lastname: Holst
authors.forename: Katarzyna A
authors.initials: KA
authors.lastname: Duda
mesh_terms: D000818:Animals; D002240:Carbohydrate Sequence; D002417:Cattle; D002473:Cell Wall / Q000737:chemistry*; D002852:Chromatography, Ion Exchange; D003837:Deoxy Sugars / Q000737:chemistry* / Q000302:isolation & purification; D005260:Female; D009682:Magnetic Resonance Spectroscopy; D008351:Mannans / Q000737:chemistry* / Q000302:isolation & purification; D008414:Mastitis, Bovine / Q000382:microbiology; D008969:Molecular Sequence Data; D009113:Muramidase / Q000737:chemistry; D013291:Streptococcus / Q000737:chemistry* / Q000302:isolation & purification; D013682:Teichoic Acids / Q000302:isolation & purification
chemical_list: D003837:Deoxy Sugars; D008351:Mannans; D013682:Teichoic Acids; C065836:rhamnan; D009113:Muramidase
keywords: Mastitis; NMR analysis; Rhamnan; SCWPs; Streptococcus uberis; WTA structure