title: Quantitative trait locus mapping identifies REME2, a PPR-DYW protein required for editing of specific C targets in Arabidopsis mitochondria.
abstract: Targeted RNA editing by C-to-U alteration occurs at hundreds of sites in the mitochondrial transcriptome of flowering plants. By using natural variation and positional cloning on a population of Arabidopsis recombinant inbred lines between the ecotypes Col and Ler, we found that two of these occurrences involve the Arabidopsis PPR-DYW protein REME2 (Required for Efficiency of Mitochondrial Editing2). The analysis of a knockdown mutant along with silenced tissues confirms the specificity of REME2 for both sites located in mitochondrial ribosomal protein genes (rps3-1534 and rps4-175). The conservation level of both cis elements is relatively high, as is the amino acid conservation among flowering plants for both genes in that location, underlining the importance of these editing events and REME2.
authors.affiliation: Department of Molecular Biology and Genetics; Cornell University; Ithaca, NY USA.
authors.forename: Stéphane
authors.initials: S
authors.lastname: Bentolila
authors.affiliation: Department of Molecular Biology and Genetics; Cornell University; Ithaca, NY USA.
authors.forename: Arianne M
authors.initials: AM
authors.lastname: Babina
authors.affiliation: Department of Molecular Biology and Genetics; Cornell University; Ithaca, NY USA.
authors.forename: Arnaud
authors.initials: A
authors.lastname: Germain
authors.affiliation: Department of Molecular Biology and Genetics; Cornell University; Ithaca, NY USA.
authors.forename: Maureen R
authors.initials: MR
authors.lastname: Hanson
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics* / Q000254:growth & development; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D004269:DNA, Bacterial; D018506:Gene Expression Regulation, Plant; D055785:Gene Knockdown Techniques; D008928:Mitochondria / Q000235:genetics*; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D020641:Polymorphism, Single Nucleotide; D040641:Quantitative Trait Loci; D017393:RNA Editing; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D012045:Regulatory Sequences, Nucleic Acid; D012269:Ribosomal Proteins / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid
chemical_list: D029681:Arabidopsis Proteins; D004269:DNA, Bacterial; D010940:Plant Proteins; C000597735:REME2 protein, Arabidopsis; D016601:RNA-Binding Proteins; C000597736:RPS3 protein, Arabidopsis; D012269:Ribosomal Proteins; C036483:T-DNA; C097421:rps4 protein, plant
keywords: QTL mapping; RPS3; RPS4; plant mitochondria; virus-induced gene silencing