title: Repetitive succinic acid production from lignocellulose hydrolysates by enhancement of ATP supply in metabolically engineered Escherichia coli.
abstract: In this study, repetitive production of succinic acid from lignocellulose hydrolysates by enhancement of ATP supply in metabolically engineered E. coli is reported. Escherichia coli BA305, a pflB, ldhA, ppc, and ptsG deletion strain overexpressing ATP-forming phosphoenolpyruvate (PEP) carboxykinase (PEPCK), produced a final succinic acid concentration of 83 g L(-1) with a high yield of 0.87 g g(-1) total sugar in 36 h of three repetitive fermentations of sugarcane bagasse hydrolysate. Furthermore, simultaneous consumption of glucose and xylose was achieved, and the specific productivity and yield of succinic acid were almost maintained constant during the repetitive fermentations.
authors.affiliation: State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing 211816, China.
authors.forename: Liya
authors.initials: L
authors.lastname: Liang
authors.forename: Rongming
authors.initials: R
authors.lastname: Liu
authors.forename: Feng
authors.initials: F
authors.lastname: Li
authors.forename: Mingke
authors.initials: M
authors.lastname: Wu
authors.forename: Kequan
authors.initials: K
authors.lastname: Chen
authors.forename: Jiangfeng
authors.initials: J
authors.lastname: Ma
authors.forename: Min
authors.initials: M
authors.lastname: Jiang
authors.forename: Ping
authors.initials: P
authors.lastname: Wei
authors.forename: Pingkai
authors.initials: P
authors.lastname: Ouyang
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D005285:Fermentation; D005818:Genetic Engineering; D006868:Hydrolysis; D008031:Lignin / Q000378:metabolism*; D019802:Succinic Acid / Q000378:metabolism*
chemical_list: C036909:lignocellulose; D000255:Adenosine Triphosphate; D008031:Lignin; D019802:Succinic Acid
keywords: ATP; Escherichia coli BA305; Lignocellulose hydrolysates; Repetitive production of succinic acid