title: [Inhibition effects of black rice pericarp extracts on cell proliferation of PC-3 cells].
abstract: OBJECTIVE
  To observe the inhibitive effects of black rice pericarp extracts on cell proliferation of human prostate cancer cell PC-3 and to explore its effecting mechanism.
  
  
  METHODS
  The black rice pericarp extract was used to treat the PC-3 cells. The inhibitory effect of black rice pericarp extract on cells proliferation of PC-3 was tested by MTT method. Cell apoptosis rates and cell cycle were measured by flow cytometric assay (FCM). Western blot was used to study the protein expression levels of p38, p-p38, JNK, p-JNK.
  
  
  RESULTS
  A dose-dependent and time-dependent proliferation inhibition of black rice pericarp extract was demonstrated in PC-3. The most prominent experiment condition was inhibitory concentration with 300microg/ml and treated for 72 h. The experiment result of flow cytometry analysis demonstrates that the apoptosis rate of PC-3 cells increased along with the increasing of black rice pericarp extract concentration, and a G1-S cell cycle arrest was induced in a dose-dependent manner. After PC-3 cell was treated with black rice pericarp extract for 72 h, the expressions of p-p38, p-JNK protein increased.
  
  
  CONCLUSIONS
  Black rice pericarp extract could inhibit proliferation, change the cell cycle distributions and induce apoptosis in human prostatic cancer cell PC-3. Its inhibitory effect may be through promoting activation of the JNK, p38 signaling pathway. These results suggest that black rice pericarp extract maybe has an inhibitory effect on prostatic cancer.
authors.affiliation: Longkou Center for Disease Control and Prevention, Longkou 265701, China. jiangweiwei78@sina.com
authors.forename: Weiwei
authors.initials: W
authors.lastname: Jiang
authors.forename: Xudong
authors.initials: X
authors.lastname: Yu
authors.forename: Guofeng
authors.initials: G
authors.lastname: Ren
mesh_terms: D000872:Anthocyanins / Q000302:isolation & purification / Q000494:pharmacology; D000970:Antineoplastic Agents / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D005960:Glucosides / Q000302:isolation & purification / Q000494:pharmacology; D006801:Humans; D008297:Male; D012275:Oryza / Q000737:chemistry* / Q000145:classification; D010936:Plant Extracts / Q000494:pharmacology*; D011471:Prostatic Neoplasms / Q000473:pathology*
chemical_list: D000872:Anthocyanins; D000970:Antineoplastic Agents; D005960:Glucosides; D010936:Plant Extracts; C462279:cyanidin-3-O-beta-glucopyranoside; C480521:peonidin 3-glucoside