title: Adipose tissue-derived mesenchymal stem cells: isolation, expansion, and characterization.
abstract: Over the last decade, cell therapy has emerged as a potentially new approach for the treatment of cardiovascular diseases. Among the wide range of cell types and sources, adipose-derived mesenchymal stem cells have shown promise, mainly due to its plasticity and remarkable paracrine-secretion capacity, largely demonstrated at the in vitro and in vivo levels. Furthermore, its accessibility and abundance, the low morbidity of the surgical procedure, its easy isolation, culture, and long-term passaging capacity added to its immunomodulatory properties that could allow its allogeneic transplantation, making it one of the most attractive candidates for clinical application. In this chapter, we will focus on the methodology for the isolation, expansion, phenotypical characterization, differentiation, and storage of the adipose-derived stem cells.
authors.affiliation: Laboratory of Cell Therapy, Division of Cancer, Foundation for Applied Medical Research, Clínica Universidad de Navarra, University of Navarra, Pamplona, Spain.
authors.forename: Miriam
authors.initials: M
authors.lastname: Araña
authors.forename: Manuel
authors.initials: M
authors.lastname: Mazo
authors.forename: Pablo
authors.initials: P
authors.lastname: Aranda
authors.forename: Beatriz
authors.initials: B
authors.lastname: Pelacho
authors.forename: Felipe
authors.initials: F
authors.lastname: Prosper
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D015415:Biomarkers / Q000032:analysis*; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D002469:Cell Separation / Q000379:methods*; D015925:Cryopreservation; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D051379:Mice; D051381:Rats; D013552:Swine
chemical_list: D015415:Biomarkers