title: Polymorphism in PGLYRP-1 gene by PCR-RFLP and its association with somatic cell score in Chinese Holstein.
abstract: Bovine peptidoglycan recognition protein 1 (PGLYRP-1), an important pattern recognition molecule (PRM) of the innate immune system, is an effector molecule in killing different microorganisms directly. To investigate whether the PGLYRP-1 gene was associated with mastitis and milk production traits in dairy cattle, the polymorphism of this gene was analyzed by PCR-RFLP in a population of 524 Chinese Holstein. A total of ten single nucleotide polymorphism (SNP) loci were identified. The association analysis of single SNP locus showed that T-35A, T-12G and G+102C were significantly associated (P<0.05) with somatic cell score (SCS), while G+102C and G+649C were significantly associated (P<0.05) with 305-day milk yield. Association analysis between combined haplotypes and SCS, milk production traits indicated that H3H3 was associated with the lower SCS (P<0.01), and H2H2 was associated with the lower 305-day milk yield (P<0.01). These findings demonstrated that polymorphisms in PGLYRP-1 gene associated with mastitis resistance and 305-day milk yield, and the H3H3 would provide a useful genetic marker of combined haplotypes for mastitis resistance selection and breeding in Chinese Holstein.
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, PR China.
authors.forename: H L
authors.initials: HL
authors.lastname: Wang
authors.forename: Z X
authors.initials: ZX
authors.lastname: Li
authors.forename: L J
authors.initials: LJ
authors.lastname: Wang
authors.forename: H
authors.initials: H
authors.lastname: He
authors.forename: J
authors.initials: J
authors.lastname: Yang
authors.forename: L
authors.initials: L
authors.lastname: Chen
authors.forename: F B
authors.initials: FB
authors.lastname: Niu
authors.forename: Y
authors.initials: Y
authors.lastname: Liu
authors.forename: J Z
authors.initials: JZ
authors.lastname: Guo
authors.forename: X L
authors.initials: XL
authors.lastname: Liu
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D002417:Cattle; D005260:Female; D006239:Haplotypes; D008414:Mastitis, Bovine / Q000235:genetics*; D008892:Milk / Q000166:cytology*; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary*; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length
chemical_list: D002352:Carrier Proteins; C118897:peptidoglycan recognition protein
keywords: Mastitis; PCR-PIRA; PCR-RFLP; PGLYRP-1 gene; Polymorphism; SCC