title: Chemopreventive and metabolic effects of inulin on colon cancer development.
abstract: Prebiotics modulate microbial composition and ensure a healthy gastrointestinal tract environment that can prevent colon cancer development. These natural dietary compounds are therefore potential chemopreventive agents. Thirty Sprague-Dawley rats (4 months old) were experimentally treated with procarcinogen dimethylhydrazine to induce colon cancer development. The rats were randomly assigned to three groups: a control group (CG), a group treated with dimethylhydrazine (DMH), and a group given DMH and inulin, a prebiotic (DMH+PRE). The effects of inulin on the activities of bacterial glycolytic enzymes, short-chain fatty acids, coliform and lactobacilli counts, cytokine levels, and cyclooxygenase-2 (COX-2) and transcription nuclear factor kappa beta (NFκB) immunoreactivity were measured. Inulin significantly decreased coliform counts (p < 0.01), increased lactobacilli counts (p < 0.001), and decreased the activity of β-glucuronidase (p < 0.01). Butyric and propionic concentrations were decreased in the DMH group. Inulin increased its concentration that had been reduced by DMH. Inulin decreased the numbers of COX-2- and NFκB-positive cells in the tunica mucosae and tela submucosae of the colon. The expression of IL-2, TNFα, and IL-10 was also diminished. This 28-week study showed that dietary intake of inulin prevents preneoplastic changes and inflammation that promote colon cancer development.
authors.affiliation: Institute of Experimental Medicine, Faculty of Medicine, Pavol Jozef S̆fárik University, Kos̆ice 040 11, The Slovak Republic. emilia.hijova@upjs.sk.
authors.forename: Emília
authors.initials: E
authors.lastname: Hijová
authors.forename: Viktória
authors.initials: V
authors.lastname: Szabadosova
authors.forename: Jana
authors.initials: J
authors.lastname: Štofilová
authors.forename: Gabriela
authors.initials: G
authors.lastname: Hrčková
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D003106:Colon / Q000201:enzymology; D003110:Colonic Neoplasms / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism; D015169:Colony Count, Microbial; D051546:Cyclooxygenase 2 / Q000235:genetics / Q000378:metabolism; D016207:Cytokines / Q000097:blood / Q000235:genetics; D004032:Diet; D019587:Dietary Supplements / Q000032:analysis; D004127:Dimethylhydrazines / Q000633:toxicity; D004755:Enterobacteriaceae / Q000187:drug effects / Q000502:physiology; D005232:Fatty Acids, Volatile / Q000235:genetics / Q000378:metabolism; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D007444:Inulin / Q000008:administration & dosage / Q000378:metabolism*; D007777:Lactobacillaceae / Q000187:drug effects / Q000502:physiology; D008297:Male; D016328:NF-kappa B / Q000235:genetics / Q000378:metabolism; D056692:Prebiotics / Q000032:analysis*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D001426:Bacterial Proteins; D016207:Cytokines; D004127:Dimethylhydrazines; D005232:Fatty Acids, Volatile; D016328:NF-kappa B; D056692:Prebiotics; D007444:Inulin; D051546:Cyclooxygenase 2; C489691:Ptgs2 protein, rat
keywords: Sprague-Dawley rats; chemoprevention; colon cancer; prebiotic