title: The neurogenic factor NeuroD1 is expressed in post-mitotic cells during juvenile and adult Xenopus neurogenesis and not in progenitor or radial glial cells.
abstract: In contrast to mammals that have limited proliferation and neurogenesis capacities, the Xenopus frog exhibit a great potential regarding proliferation and production of new cells in the adult brain. This ability makes Xenopus a useful model for understanding the molecular programs required for adult neurogenesis. Transcriptional factors that control adult neurogenesis in vertebrate species undergoing widespread neurogenesis are unknown. NeuroD1 is a member of the family of proneural genes, which function during embryonic neurogenesis as a potent neuronal differentiation factor. Here, we study in detail the expression of NeuroD1 gene in the juvenile and adult Xenopus brains by in situ hybridization combined with immunodetections for proliferation markers (PCNA, BrdU) or in situ hybridizations for cell type markers (Vimentin, Sox2). We found NeuroD1 gene activity in many brain regions, including olfactory bulbs, pallial regions of cerebral hemispheres, preoptic area, habenula, hypothalamus, cerebellum and medulla oblongata. We also demonstrated by double staining NeuroD1/BrdU experiments, after long post-BrdU administration survival times, that NeuroD1 gene activity was turned on in new born neurons during post-metamorphic neurogenesis. Importantly, we provided evidence that NeuroD1-expressing cells at this brain developmental stage were post-mitotic (PCNA-) cells and not radial glial (Vimentin+) or progenitors (Sox2+) cells.
authors.affiliation: Centre de Ressources Biologiques Xénope UMS U3387, CNRS, and Rennes 1 University, Rennes, France.
authors.forename: Laure Anne
authors.initials: LA
authors.lastname: D'Amico
authors.forename: Daniel
authors.initials: D
authors.lastname: Boujard
authors.forename: Pascal
authors.initials: P
authors.lastname: Coumailleau
mesh_terms: D000818:Animals; D000943:Antigens, Differentiation / Q000235:genetics / Q000378:metabolism; D051792:Basic Helix-Loop-Helix Proteins / Q000235:genetics / Q000378:metabolism*; D049109:Cell Proliferation; D002478:Cells, Cultured; D002531:Cerebellum / Q000166:cytology / Q000378:metabolism; D004027:Diencephalon / Q000166:cytology / Q000378:metabolism; D063928:Ependymoglial Cells / Q000378:metabolism*; D005260:Female; D015870:Gene Expression; D018507:Gene Expression Regulation, Developmental; D008297:Male; D008675:Metamorphosis, Biological; D008938:Mitosis; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D058953:Neural Stem Cells / Q000378:metabolism*; D055495:Neurogenesis; D009928:Organ Specificity; D055748:SOXB1 Transcription Factors / Q000378:metabolism; D013687:Telencephalon / Q000166:cytology / Q000378:metabolism; D015854:Up-Regulation; D014746:Vimentin / Q000378:metabolism; D029867:Xenopus Proteins / Q000378:metabolism; D014982:Xenopus laevis / Q000502:physiology*
chemical_list: D000943:Antigens, Differentiation; D051792:Basic Helix-Loop-Helix Proteins; D009419:Nerve Tissue Proteins; D055748:SOXB1 Transcription Factors; D014746:Vimentin; D029867:Xenopus Proteins; C527781:sox2 protein, Xenopus; C093564:Neurogenic differentiation factor 1