title: Cloning and molecular characterization of cationic amino acid transporter y⁺LAT1 in grass carp (Ctenopharyngodon idellus).
abstract: The solute carrier family 7A, member 7 gene encodes the light chain- y⁺L amino acid transporter-1 (y⁺LAT1) of the heterodimeric carrier responsible for cationic amino acid (CAA) transport across the basolateral membranes of epithelial cells in intestine and kidney. Rising attention has been given to y⁺LAT1 involved in CAA metabolic pathways and growth control. The molecular characterization and function analysis of y⁺LAT1 in grass carp (Ctenopharyngodon idellus) is currently unknown. In the present study, full-length cDNA (2,688 bp), which encodes y⁺LAT1 and contains a 5'-untranslated region (319 bp), an open reading frame (1,506 bp) and a 3'-untranslated region (863 bp), has been cloned from grass carp. Amino acid sequence of grass carp y⁺LAT1 contains 11 transmembrane domains and shows 95 %, 80 % and 75 % sequence similarity to zebra fish, amphibian and mammalian y⁺LAT1, respectively. The tissue distribution and expression regulation by fasting of y⁺LAT1 mRNA were analyzed using real-time PCR. Our results showed that y⁺LAT1 mRNA was highly expressed in midgut, foregut and spleen while weakly expressed in hindgut, kidney, gill, brain, heart, liver and muscle. Nutritional status significantly influenced y⁺LAT1 mRNA expression in fish tissues, such as down-regulation of y⁺LAT1 mRNA expression after fasting (14 days).
authors.affiliation: Fisheries College, Huazhong Agricultural University, Wuhan, 430070, China.
authors.forename: Jixuan
authors.initials: J
authors.lastname: Yang
authors.forename: Qingsong
authors.initials: Q
authors.lastname: Tan
authors.forename: Wenhuan
authors.initials: W
authors.lastname: Zhu
authors.forename: Chen
authors.initials: C
authors.lastname: Chen
authors.forename: Xufang
authors.initials: X
authors.lastname: Liang
authors.forename: Lei
authors.initials: L
authors.lastname: Pan
mesh_terms: D000595:Amino Acid Sequence; D027241:Amino Acid Transport System y+L / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D001483:Base Sequence; D002347:Carps / Q000235:genetics / Q000378:metabolism*; D005215:Fasting / Q000378:metabolism; D029941:Fish Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D027283:Large Neutral Amino Acid-Transporter 1 / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D010802:Phylogeny; D011897:Random Allocation; D060888:Real-Time Polymerase Chain Reaction; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
chemical_list: D027241:Amino Acid Transport System y+L; D029941:Fish Proteins; D027283:Large Neutral Amino Acid-Transporter 1