title: Early infection HIV-1 envelope V1-V2 genotypes do not enhance binding or replication in cells expressing high levels of α4β7 integrin.
abstract: It has been postulated that HIV-1 envelope properties, such as shorter and less-glycosylated V1-V2 loops commonly observed among non-subtype B early-transmitted viruses, promote utilization of the gut homing integrin α4β7. This property potentially confers an advantage to some HIV-1 variants early after acquisition. We found that replication-competent recombinant viruses incorporating HIV-1 subtype A compact and less-glycosylated early versus chronic phase V1-V2 loops demonstrated no significant difference in binding to α4β7 high CD8⁺ T cells or replication in α4β7 high CD4⁺ T cells. Integrin α4β7 usage does not select for shorter less-glycosylated envelopes during transmission.
authors.affiliation: *Department of Medicine, Division of Infectious Disease, Boston University School of Medicine, Boston, MA; and †Dana Farber Cancer Research Center, Boston, MA.
authors.forename: Behzad
authors.initials: B
authors.lastname: Etemad
authors.forename: Oscar A
authors.initials: OA
authors.lastname: Gonzalez
authors.forename: Sean
authors.initials: S
authors.lastname: McDonough
authors.forename: Victor
authors.initials: V
authors.lastname: Pena-Cruz
authors.forename: Manish
authors.initials: M
authors.lastname: Sagar
mesh_terms: D000595:Amino Acid Sequence; D001665:Binding Sites; D015496:CD4-Positive T-Lymphocytes / Q000378:metabolism*; D018414:CD8-Positive T-Lymphocytes / Q000378:metabolism*; D002478:Cells, Cultured; D005260:Female; D005753:Gastric Mucosa / Q000276:immunology; D005838:Genotype; D006031:Glycosylation; D015658:HIV Infections / Q000235:genetics / Q000276:immunology* / Q000503:physiopathology; D015497:HIV-1 / Q000235:genetics / Q000276:immunology*; D006801:Humans; D018928:Immunity, Mucosal; D016023:Integrins / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D008137:Longitudinal Studies; D008297:Male; D014759:Viral Envelope Proteins / Q000235:genetics / Q000276:immunology; D014779:Virus Replication
chemical_list: D016023:Integrins; D014759:Viral Envelope Proteins; C071777:integrin alpha4beta7