title: [Dynamics of change of ureaplasma laboratory strain titers and quantity of their DNA in transport medium at varying temperature].
abstract: OBJECTIVE
  Study of preservation dynamics of ureaplasma laboratory strain live cultures and their DNA in transport medium at varying temperature.
  
  
  METHODS
  The study was carried out in laboratory strains Ureaplasma urealyticum serotype 8 and Ureaplasma parvum serotype 1. The quantity of live ureaplasmas was determined by method of tenfold dilutions in liquid medium. The growth of ureaplasmas was registered by changes in the color of the cultivation medium due to its alkalization by metabolism products and expressed in CCU/ml. DNA quantity in samples was determined by real time PCR performed by using Florocenosis-micoplasmas-FL test system produced by ILS.
  
  
  RESULTS
  Live ureaplasmas wer shown to be preserved in transport medium at 4 degrees C for 12 - 29 days, at 18 - 22 degrees C--for 9 - 20 days and at 37 degrees C--for only 2 days. In samples incubated at 37 degrees C the quantity of live ureaplasmas increased and then sharply decreased to 0, at lower temperature titers of the cells decreased smoothly. The quantity of ureaplasma DNA in the process of their incubation did not change significantly.
  
  
  CONCLUSIONS
  Fundamental differences in the duration of survival of U. urealyticum strain and U. parvum strain in transport medium at varying temperature were not detected. Based on the studies performed a practical conclusion can be drawn that in cases of emergency when clinical material transportation is necessary its storage in transport medium for several days is acceptable.
authors.forename: N A
authors.initials: NA
authors.lastname: Gamova
authors.forename: T A
authors.initials: TA
authors.lastname: Ivanova
mesh_terms: D058491:Bacterial Load; D003470:Culture Media; D004269:DNA, Bacterial / Q000235:genetics*; D006801:Humans; D050296:Microbial Viability; D011309:Preservation, Biological / Q000379:methods*; D060888:Real-Time Polymerase Chain Reaction; D012703:Serotyping; D013696:Temperature; D014509:Ureaplasma / Q000145:classification / Q000235:genetics / Q000254:growth & development* / Q000302:isolation & purification; D016869:Ureaplasma Infections / Q000382:microbiology; D016990:Ureaplasma urealyticum / Q000145:classification / Q000235:genetics / Q000254:growth & development* / Q000302:isolation & purification
chemical_list: D003470:Culture Media; D004269:DNA, Bacterial