title: Systematic assignment of NMR spectra of 5-substituted-4-thiopyrimidine nucleosides.
abstract: Unambiguous characterization of 5-substituted-4-thiopyrimidine nucleosides (ribonucleosides and 2'-deoxynucleosides) was performed using NMR spectroscopy. Assignments of all proton and carbon signals of 5-bromo-4-thiouridine and related nucleosides were systematically carried out and firmly established by COSY and HMQC techniques. The NMR data of various 4-thiopyrimidine nucleosides are compared, and the key contributing factors discussed. The approach presented here is applicable to other modified nucleosides and nucleotides, as well as nucleobases.
authors.affiliation: College of Environment and Chemical Engineering, Dalian University, Dalian 116622, China. zhangxiaohui@dlu.edu.cn
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Zhang
authors.forename: Jian
authors.initials: J
authors.lastname: Wang
authors.forename: Yao-Zhong
authors.initials: YZ
authors.lastname: Xu
mesh_terms: D009682:Magnetic Resonance Spectroscopy / Q000592:standards; D011741:Pyrimidine Nucleosides / Q000138:chemical synthesis / Q000737:chemistry*; D012015:Reference Standards
chemical_list: D011741:Pyrimidine Nucleosides
keywords: 13C; 1H; 4-thiothymidine; 4-thiouridine; 5-bromo-4-thiouridine; 5-substituted pyrimidine; DNA; NMR; RNA; nucleoside