title: Toward single cell traction microscopy within 3D collagen matrices.
abstract: Mechanical interaction between the cell and its extracellular matrix (ECM) regulates cellular behaviors, including proliferation, differentiation, adhesion, and migration. Cells require the three-dimensional (3D) architectural support of the ECM to perform physiologically realistic functions. However, current understanding of cell-ECM and cell-cell mechanical interactions is largely derived from 2D cell traction force microscopy, in which cells are cultured on a flat substrate. 3D cell traction microscopy is emerging for mapping traction fields of single animal cells embedded in either synthetic or natively derived fibrous gels. We discuss here the development of 3D cell traction microscopy, its current limitations, and perspectives on the future of this technology. Emphasis is placed on strategies for applying 3D cell traction microscopy to individual tumor cell migration within collagen gels.
authors.affiliation: Department of Biological and Environmental Engineering, Cornell University, Ithaca, NY 14853, USA.
authors.forename: Matthew S
authors.initials: MS
authors.lastname: Hall
authors.forename: Rong
authors.initials: R
authors.lastname: Long
authors.forename: Xinzeng
authors.initials: X
authors.lastname: Feng
authors.forename: Yuling
authors.initials: Y
authors.lastname: Huang
authors.forename: Chung-Yuen
authors.initials: CY
authors.lastname: Hui
authors.forename: Mingming
authors.initials: M
authors.lastname: Wu
mesh_terms: D000818:Animals; D002448:Cell Adhesion / Q000502:physiology; D002465:Cell Movement / Q000502:physiology; D003094:Collagen / Q000378:metabolism / Q000648:ultrastructure*; D005109:Extracellular Matrix / Q000378:metabolism*; D006801:Humans; D018625:Microscopy, Atomic Force; D018613:Microscopy, Confocal
chemical_list: D003094:Collagen
keywords: 2D; 3D; 3D imaging; AFM; Cell mechanics; Cell migration; Collagen; DMEM; Dulbecco's modified Eagle medium; ECM; Extracellular matrix; FM; PDMS; PEG; TFM; Traction force microscopy; atomic force microscopy; extracellular matrix; polydimethylsiloxane; polyethylene glycol; three-dimensional; traction force microscopy; two-dimensional; widefield fluorescence microscopy