title: Black rice anthocyanidins prevent retinal photochemical damage via involvement of the AP-1/NF-κB/Caspase-1 pathway in Sprague-Dawley rats.
abstract: The effects of black rice anthocyanidins (BRACs) on retinal damage induced by photochemical stress are not well known. In the present study, Sprague-Dawley rats were fed AIN-93M for 1 week, after which 80 rats were randomly divided into two groups and treated with (n = 40) or without BRACs (n = 40) for 15 days, respectively. After treatment, both groups were exposed to fluorescent light (3,000 ± 200 lux; 25°C), and the protective effect of dietary BRACs were evaluated afterwards. Our results showed that dietary BRACs effectively prevented retinal photochemical damage and inhibited the retinal cells apoptosis induced by fluorescent light (p < 0.05). Moreover, dietary BRACs inhibited expression of AP-1 (c-fos/c-jun subunits), up-regulated NF-κB (p65) expression and phosphorylation of IκB-α, and decreased Caspase-1 expression (p < 0.05). These results suggest that BRACs improve retinal damage produced by photochemical stress in rats via AP-1/NF-κB/Caspase-1 apoptotic mechanisms.
authors.affiliation: Department of Public Health, Chengdu Medical College, Chengdu City, 610050, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Jia
authors.forename: Wei
authors.initials: W
authors.lastname: Chen
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Yu
authors.forename: Xiuhua
authors.initials: X
authors.lastname: Wu
authors.forename: Shuai
authors.initials: S
authors.lastname: Li
authors.forename: Hong
authors.initials: H
authors.lastname: Liu
authors.forename: Jiru
authors.initials: J
authors.lastname: Liao
authors.forename: Weihua
authors.initials: W
authors.lastname: Liu
authors.forename: Mantian
authors.initials: M
authors.lastname: Mi
authors.forename: Longjian
authors.initials: L
authors.lastname: Liu
authors.forename: Daomei
authors.initials: D
authors.lastname: Cheng
mesh_terms: D000821:Animal Feed / Q000032:analysis; D000818:Animals; D000872:Anthocyanins / Q000008:administration & dosage / Q000494:pharmacology*; D000975:Antioxidants / Q000008:administration & dosage / Q000502:physiology*; D015153:Blotting, Western; D020170:Caspase 1 / Q000235:genetics* / Q000378:metabolism; D004032:Diet; D019587:Dietary Supplements / Q000032:analysis; D020672:I-kappa B Proteins / Q000235:genetics / Q000378:metabolism; D000072000:NF-KappaB Inhibitor alpha; D016328:NF-kappa B / Q000235:genetics* / Q000378:metabolism; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism; D029543:Nucleocytoplasmic Transport Proteins / Q000235:genetics / Q000378:metabolism; D012275:Oryza / Q000737:chemistry; D016760:Proto-Oncogene Proteins c-fos / Q000235:genetics / Q000378:metabolism; D016755:Proto-Oncogene Proteins c-jun / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D060888:Real-Time Polymerase Chain Reaction; D012164:Retinal Diseases / Q000209:etiology / Q000517:prevention & control*; D015398:Signal Transduction / Q000187:drug effects* / Q000528:radiation effects; D018808:Transcription Factor AP-1 / Q000235:genetics* / Q000378:metabolism
chemical_list: D000872:Anthocyanins; D000975:Antioxidants; D020672:I-kappa B Proteins; D016328:NF-kappa B; D009363:Neoplasm Proteins; C000606206:Nfkbia protein, rat; D029543:Nucleocytoplasmic Transport Proteins; D016760:Proto-Oncogene Proteins c-fos; D016755:Proto-Oncogene Proteins c-jun; D018808:Transcription Factor AP-1; C063447:p65 oncofetal mRNA transport protein, rat; D000072000:NF-KappaB Inhibitor alpha; D020170:Caspase 1
keywords: Caspase-1; apoptosis; black rice anthocyanidins; photochemical damage; retina