title: [Effects of mannan-binding lectin on the functions of human polymorphonuclear cells].
abstract: OBJECTIVE
  To investigate the effects of mannan-binding lectin (MBL) on the functions of human polymorphonuclear cells (PMNs).
  
  
  METHODS
  ELISA and Dot blot were performed to examine the binding between MBL and the microorganisms. Flow cytometry and fluorescence microscopy were employed to analyze the phagocytosis of FITC-labeled microorganisms by the PMNs. Real-time quantitative PCR was used to detect the expression levels of IL-1β, TNF-α and CD11b mRNA in the PMNs, and ELISA used to detect the levels of TNF-α and IL-6 in the supernatants of PMN culture. Nitro-blue tetrazolium reduction assay was used to estimate the levels of superoxide production.
  
  
  RESULTS
  MBL bound to the microorganisms in a dose-dependent manner. MBL had no significant effect on phagocytosis of C. albicans and E.coli by the PMNs in the absence of human serum, but in presence of mixed MBL-deficient human sera, MBL promoted the phagocytosis of C. albicans, which could be blocked by mannan. Mannan treatment increased the expressions of IL-1β, TNF-α, IL-6 and CD11b and enhanced superoxide production in the PMNs.
  
  
  CONCLUSIONS
  MBL can promote phagocytosis of microorganisms by PMNs and increase the expressions of proinflammatory cytokines from PMNs in a complement lectin pathway-dependent manner.
authors.affiliation: Department of Immunology, Southern Medical University, Guangzhou 510515, China. chenade@163.com
authors.forename: Ade
authors.initials: A
authors.lastname: Chen
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.forename: Liyun
authors.initials: L
authors.lastname: Zhang
authors.forename: Xiao
authors.initials: X
authors.lastname: Lu
authors.forename: Daming
authors.initials: D
authors.lastname: Zuo
authors.forename: Zhengliang
authors.initials: Z
authors.lastname: Chen
mesh_terms: D002176:Candida albicans / Q000276:immunology; D002478:Cells, Cultured; D016207:Cytokines / Q000276:immunology; D004926:Escherichia coli / Q000276:immunology; D006801:Humans; D037601:Mannose-Binding Lectin / Q000097:blood*; D009504:Neutrophils / Q000276:immunology*; D010587:Phagocytosis; D013481:Superoxides / Q000276:immunology
chemical_list: D016207:Cytokines; D037601:Mannose-Binding Lectin; D013481:Superoxides