title: [Effects of T-2 toxin of Fusarium on proliferation and apoptosis of human acute promyelocytic leukemia cell line HL60].
abstract: OBJECTIVE
  To study the effects of sesquiterpenes compounds T-2 toxin produced by Fusarium fungi on proliferation and apoptosis of acute promyelocytic leukemia cellline HL60.
  
  
  METHODS
  HL60 cells were treated with T-2 toxin (0, 4, 8, 16 and 32 microg/ml) for 48 h, and then cells were harvested for the studies of growth inhibition with MTT, Cell morphology with Giemsa staining, apoptosis and the expression of Bax and Bcl-2 with flowcytometry (FCM) and Western blot.
  
  
  RESULTS
  MTT results showed that T-2 toxin inhibited HL60 proliferation on dose-dependent. Morphological observation showed significant apoptotic morphological characteristics. FCM results showed that the apoptosis rates of HL60 cells in T-2 toxin treatment groups were higher than that in control, and positive dose-effect correlations could be found. FCM and Western blot results showed that the expression of Bax was increased while that of Bcl-2 was decreased in T-2 toxin treatment groups of HL60.
  
  
  CONCLUSIONS
  The results suggested that T-2 toxin could inhibite proliferation and induce apoptosis of HL60 cells in vitro in a dose-dependent manner.
authors.affiliation: Department of Medical Laboratory, Jilin Medical College, Jilin, China. houyiju@163.com
authors.forename: Yiju
authors.initials: Y
authors.lastname: Hou
authors.forename: Zhonghai
authors.initials: Z
authors.lastname: Yuan
authors.forename: Wei
authors.initials: W
authors.lastname: Guo
authors.forename: Huiyan
authors.initials: H
authors.lastname: Wang
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D018922:HL-60 Cells; D006801:Humans; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D013605:T-2 Toxin / Q000494:pharmacology*; D051028:bcl-2-Associated X Protein / Q000378:metabolism
chemical_list: C494826:BAX protein, human; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein; D013605:T-2 Toxin