title: Markers suggest reduced malignant potential of subsquamous intestinal metaplasia compared with Barrett's esophagus.
abstract: Esophageal subsquamous intestinal metaplasia (SSIM) is frequently observed in patients with Barrett's esophagus (BE) and can also be found in patients after endoscopic ablative treatments for dysplastic BE. While these 'buried glands' appear identical to BE glands, features of SSIM and its malignant potency have yet to be fully elucidated. To determine differences in malignant potential between nondysplastic BE and SSIM, the Automated Cellular Imaging System was used to assess and compare changes in DNA content between SSIM and BE. Samples were further immunostained for Ki67 and Lgr5 to gauge general proliferative and possible stem cell features, respectively, in SSIM cells compared with BE glands. No significant differences were found between SSIM and BE with regards to DNA ploidy aberrance. However, significant differences were noted between SSIM and BE upon immunohistochemical analysis. SSIM was found to be negative for both Ki67 and Lgr5 while BE was positive for both markers. SSIM cells appear to be relatively quiescent and behave differently from BE, suggesting a reduced proclivity toward cancer progression.
authors.affiliation: VA Boston Healthcare System, Boston, MA, USA.
authors.forename: M
authors.initials: M
authors.lastname: Basavappa
authors.forename: A
authors.initials: A
authors.lastname: Weinberg
authors.forename: Q
authors.initials: Q
authors.lastname: Huang
authors.forename: H
authors.initials: H
authors.lastname: Mashimo
mesh_terms: D000782:Aneuploidy; D001471:Barrett Esophagus / Q000235:genetics / Q000473:pathology*; D015415:Biomarkers / Q000032:analysis; D004247:DNA / Q000032:analysis*; D018450:Disease Progression; D004947:Esophagus / Q000737:chemistry / Q000473:pathology*; D006801:Humans; D019394:Ki-67 Antigen / Q000032:analysis*; D008679:Metaplasia / Q000235:genetics / Q000473:pathology; D011230:Precancerous Conditions / Q000737:chemistry* / Q000473:pathology*; D043562:Receptors, G-Protein-Coupled / Q000032:analysis*
chemical_list: D015415:Biomarkers; D019394:Ki-67 Antigen; C116884:LGR5 protein, human; D043562:Receptors, G-Protein-Coupled; D004247:DNA
keywords: Barrett's esophagus; Ki67; Lgr5; dysplasia; subsquamous intestinal metaplasia