title: [Screening of differentially expressed proteins in serum from subjects with Keshan disease by two-dimensional electrophoresis and mass and mass spectrometry].
abstract: OBJECTIVE
  To screen differentially expressed proteins in serum in patients with Keshan disease (KD), peripheral blood protein expression spectrum between subjects with Keshan disease and health controls were compared.
  
  
  METHODS
  Differentially expressed protein spots were screened by two-dimensional gel electrophoresis (2-DE) between Keshan disease and health control subjects, and constitutive protein were identified by matrix assisted laser adsorption/ionization-time of flight mass spectrometry (MALDI-TOF-MS).
  
  
  RESULTS
  9 differentially expressed protein spots were showed in 2-DE images and 8 differentially expressed proteins were identified by MALDI-TOF-MS. In them, 3 up-regulated proteins, mainly relatedd to lipid metabolism, apoptosis resistance, immunological regulation and 3 down-regulated proteins, involved to cellular iron ion homeostasis; 2 up-regulated proteins in serum in patients with KD versus controls from KD areas were detected, mainly associated with protease inhibition.
  
  
  CONCLUSIONS
  Haptoglobin, serum albumin, alpha-l-antitrypsin, transferring and alpha2-heremans schmid glycoprotein may be considered as candidate biological indicators used for diagnosis or prognosis of KD.
authors.affiliation: Key Laboratory of Environment and Gene Related Diseases, Key Laboratory of Trace Elements and Endemic Diseases, Ministry of Health, Xi'an Jiaotong University, Ministry Education, Xi'an 710061, China. heshulan0954@163.com
authors.forename: Shulan
authors.initials: S
authors.lastname: He
authors.forename: Wuhong
authors.initials: W
authors.lastname: Tan
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.forename: Pan
authors.initials: P
authors.lastname: Wang
authors.forename: Youzhang
authors.initials: Y
authors.lastname: Xiang
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000097:blood; D001798:Blood Proteins / Q000032:analysis* / Q000145:classification; D009202:Cardiomyopathies / Q000097:blood*; D015180:Electrophoresis, Gel, Two-Dimensional; D004769:Enterovirus Infections / Q000097:blood*; D005260:Female; D020869:Gene Expression Profiling; D006242:Haptoglobins / Q000032:analysis; D006801:Humans; D008297:Male; D008875:Middle Aged; D020543:Proteome / Q000378:metabolism; D040901:Proteomics; D012709:Serum Albumin / Q000032:analysis; D053719:Tandem Mass Spectrometry; D061250:Transferrins / Q000032:analysis; D055815:Young Adult
chemical_list: D015415:Biomarkers; D001798:Blood Proteins; D006242:Haptoglobins; D020543:Proteome; D012709:Serum Albumin; D061250:Transferrins