title: Simultaneous Detection of Fenitrothion and Chlorpyrifos-Methyl with a Photonic Suspension Array.
abstract: A technique was developed for simultaneous detection of fenitrothion (FNT) and chlorpyrifos-methyl (CLT) using a photonic suspension array based on silica colloidal crystal beads (SCCBs). The SCCBs were encoded with the characteristic reflection peak originating from the stop-band of colloidal crystal. This approach avoids the bleaching, fading or potential interference seen when encoding by fluorescence. SCCBs with a nanopatterned surface had increased biomolecule binding capacity and improved stability. Under optimal conditions, the proposed suspension array allowed simultaneous detection of the selected pesticides in the ranges of 0.25 to 1024 ng/mL and 0.40 to 735.37 ng/mL, with the limits of detection (LODs) of 0.25 and 0.40 ng/mL, respectively. The suspension array was specific and had no significant cross-reactivity with other chemicals. The mean recoveries in tests in which samples were spiked with target standards were 82.35% to 109.90% with a standard deviation within 9.93% for CLT and 81.64% to 108.10% with a standard deviation within 8.82% for FNT. The proposed method shows a potentially powerful capability for fast quantitative analysis of pesticide residues.
authors.affiliation: Key Laboratory of Environmental Medicine Engineering, Ministry of Education, School of Public Health, Southeast University, Nanjing, Jiangsu, China.
authors.forename: Xuan
authors.initials: X
authors.lastname: Wang
authors.forename: Zhongde
authors.initials: Z
authors.lastname: Mu
authors.forename: Fengqi
authors.initials: F
authors.lastname: Shangguan
authors.forename: Ran
authors.initials: R
authors.lastname: Liu
authors.forename: Yuepu
authors.initials: Y
authors.lastname: Pu
authors.forename: Lihong
authors.initials: L
authors.lastname: Yin
mesh_terms: D004390:Chlorpyrifos / Q000031:analogs & derivatives* / Q000032:analysis; D005278:Fenitrothion / Q000032:analysis*; D007118:Immunoassay; D057230:Limit of Detection; D046228:Microarray Analysis; D010573:Pesticide Residues / Q000032:analysis*; D017785:Photons; D012822:Silicon Dioxide / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D013535:Suspensions / Q000737:chemistry
chemical_list: D010573:Pesticide Residues; D013535:Suspensions; D012822:Silicon Dioxide; D004390:Chlorpyrifos; C007031:chlorpyrifos-methyl; D005278:Fenitrothion