title: Prostaglandin E(2) and insulin-like growth factor I interact to enhance proliferation of theca externa cells from chicken prehierarchical follicles.
abstract: The interactive effect of insulin-like growth factor I (IGF-I) and prostaglandin E2 (PGE2) on the proliferation of theca externa cells (TECs) was investigated in the prehierarchical small yellow follicles of laying hens. IGF-I manifested a proliferating effect like PGE2 on TECs, but this stimulating effect was restrained by AG1024 (IGF-IR inhibitor), KP372-1 (PKB/AKT inhibitor) or NS398 (COX-2 inhibitor). AG1024, KP372-1 or NS398 abolished IGF-I-stimulated COX-2 expression and PGE2 production. Meanwhile, KP372-1, NS398 or AG1024 depressed the PGE2-stimulated expression of COX-2 and IGF-IR mRNA. Therefore, the IGF-I receptor pathway up-regulates COX-2 expression and PGE2 synthesis via PKB signaling cascade, and then PGE2 stimulates IGF-IR mRNA expression to promote TEC proliferation in an autocrine pattern. Overall, the reciprocal stimulation of intracellular PGE2 and IGF-I may enhance TEC proliferation and facilitate the development of chicken prehierarchical follicles.
authors.affiliation: Department of Veterinary Medicine, College of Animal Sciences, Zhejiang University, Hangzhou 310058, China; Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China.
authors.forename: Yudong
authors.initials: Y
authors.lastname: Jia
authors.forename: Jinxing
authors.initials: J
authors.lastname: Lin
authors.forename: Yuling
authors.initials: Y
authors.lastname: Mi
authors.forename: Caiqiao
authors.initials: C
authors.lastname: Zhang
mesh_terms: D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D002645:Chickens; D051546:Cyclooxygenase 2 / Q000235:genetics; D015232:Dinoprostone / Q000096:biosynthesis / Q000494:pharmacology*; D004347:Drug Interactions; D005260:Female; D006576:Heterocyclic Compounds, 4 or More Rings / Q000494:pharmacology; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology*; D009578:Nitrobenzenes / Q000494:pharmacology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013449:Sulfonamides / Q000494:pharmacology; D013777:Tetrazoles / Q000494:pharmacology; D013799:Theca Cells / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism; D020032:Tyrphostins / Q000494:pharmacology
chemical_list: D006576:Heterocyclic Compounds, 4 or More Rings; C501199:KP372-1; D009578:Nitrobenzenes; D012333:RNA, Messenger; D013449:Sulfonamides; D013777:Tetrazoles; D020032:Tyrphostins; C447031:tyrphostin AG 1024; C080955:N-(2-cyclohexyloxy-4-nitrophenyl)methanesulfonamide; D007334:Insulin-Like Growth Factor I; D051546:Cyclooxygenase 2; D015232:Dinoprostone
keywords: Chicken; Insulin-like growth factor I; Prehierarchical follicle; Prostaglandin E(2); Theca externa cell