title: Metabolomic Analysis of the Effect of Postnatal Hypoxia on the Retina in a Newly Born Piglet Model.
abstract: The availability of reliable biomarkers of brain injury secondary to birth asphyxia could substantially improve clinical grading, therapeutic intervention strategies, and prognosis. In this study, changes in the metabolome of retinal tissue caused by profound hypoxia in an established neonatal piglet model were investigated using an ultra performance liquid chromatography - quadrupole time of flight mass spectrometry (UPLC-QTOFMS) untargeted metabolomic approach, which included Partial Least Squares - Discriminant Analysis (PLSDA) multivariate data analysis. The initial identification of a set of discriminant metabolites from UPLC-QTOFMS data was confirmed by target UPLC-MS/MS and allowed the selection of endogenous CDP-choline as a promising candidate biomarker for hypoxia-derived brain damage assessing intensity of retinal hypoxia. Results from this study will foster further research on CDP-choline changes occurring during resuscitation.
authors.affiliation: Department of Pediatric Research, Institute for Surgical Research, Oslo University Hospital - Rikshospitalet, Oslo, Norway.
authors.forename: Rønnaug
authors.initials: R
authors.lastname: Solberg
authors.forename: Javier
authors.initials: J
authors.lastname: Escobar
authors.forename: Alessandro
authors.initials: A
authors.lastname: Arduini
authors.forename: Isabel
authors.initials: I
authors.lastname: Torres-Cuevas
authors.forename: Agustín
authors.initials: A
authors.lastname: Lahoz
authors.forename: Juan
authors.initials: J
authors.lastname: Sastre
authors.forename: Ola Didrik
authors.initials: OD
authors.lastname: Saugstad
authors.forename: Máximo
authors.initials: M
authors.lastname: Vento
authors.forename: Julia
authors.initials: J
authors.lastname: Kuligowski
authors.forename: Guillermo
authors.initials: G
authors.lastname: Quintás
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D015415:Biomarkers / Q000378:metabolism; D002853:Chromatography, Liquid; D005136:Eye Proteins / Q000378:metabolism*; D000860:Hypoxia / Q000473:pathology*; D055432:Metabolomics; D012160:Retina / Q000473:pathology*; D013552:Swine; D053719:Tandem Mass Spectrometry
chemical_list: D015415:Biomarkers; D005136:Eye Proteins