title: Flavonoids from Pseudotsuga menziesii.
abstract: Four O-acylated flavonol glycosides, new in the plant kingdom, were isolated from the needles of Pseudotsuga menziesii. Their structures were established by 1D and 2D NMR and MS data as: daglesioside I [kaempferol 3-O-[2",5"-O-(4''',4(IV)-dihydroxy)-beta-truxinoyl]-alpha-L-arabinofuranoside] (1), daglesioside II [kaempferol 3-O-[2",5"-O-(4"'-hydroxy)-beta-truxinoyl]-alpha-L-arabinofuranoside] (2), daglesioside III [kaempferol 3-O-[2",5"-di-O-(E)-p-coumaroyl]-alpha-L-arabinofuranoside] (3), and daglesioside IV [kaempferol 3-O-[3",6"-di-O-(E)-cinnamoyl]-beta-D-glucopyranoside] (4). In addition, the known flavonoids (E)-tiliroside, (E)-ditiliroside, astragalin (kaempferol 3-O-beta-D-glucopyranoside), isorhamnetin, kaempferol, and quercetin were identified. The cytotoxic activity of compounds 1 and 3 was evaluated towards the HL-60, HeLa, and MDA-MB468 cell lines.
authors.affiliation: Department of Pharmacognosy, Medical University of Gdańsk, Gen. J. Hallera 107 str., 80-416 Gdańsk, Poland. krauze@gumed.edu.pl
authors.forename: Mirosława
authors.initials: M
authors.lastname: Krauze-Baranowska
authors.forename: Paweł
authors.initials: P
authors.lastname: Sowiński
authors.forename: Anna
authors.initials: A
authors.lastname: Kawiak
authors.forename: Barbara
authors.initials: B
authors.lastname: Sparzak
mesh_terms: D045744:Cell Line, Tumor; D002855:Chromatography, Thin Layer; D005419:Flavonoids / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D006027:Glycosides / Q000737:chemistry; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D028224:Pseudotsuga / Q000737:chemistry*
chemical_list: D005419:Flavonoids; D006027:Glycosides