title: Fertilization and embryo development with spermatozoa obtained from testicular sperm extraction into oocytes generated from human chorionic gonadotropin-primed in vitro maturation cycles.
abstract: OBJECTIVE
  To evaluate the fertilization rate and embryo development resulting from intracytoplasmic sperm injection (ICSI) of spermatozoa retrieved by testicular sperm extraction (TESE) in hCG-primed in vitro maturation (IVM) cycles.
  
  
  METHODS
  Case-control study.
  
  
  METHODS
  University teaching hospital.
  
  
  METHODS
  Twenty-four IVM cycles were performed in 21 patients (mean age, 32.3 ± 2.4 years) with polycystic ovaries (PCO) whose partners were nonobstructive azoospermic. Twelve cycles where IVM oocytes were also retrieved were compared with a control group consisting of age-matched IVM cycles with ICSI using ejaculated spermatozoa (n = 12).
  
  
  METHODS
  In vitro maturation treatment with TESE sperm.
  
  
  METHODS
  Fertilization and embryo development between sibling oocytes matured in vivo and in vitro.
  
  
  RESULTS
  Eight singleton pregnancies and one twin pregnancy were obtained after ET (9/24, 37.5%). In the 12 IVM cycles where in vivo-matured oocytes were also obtained, the fertilization rate after TESE-ICSI was significantly higher in in vivo-matured oocytes than in sibling in vitro-matured oocytes (84.2% vs. 53.2%). The proportion of good quality embryos was also higher (63.5% vs. 40.2%). In the control group of cycles with ejaculated spermatozoa, there was no difference in fertilization rates between sibling oocytes matured in vivo and in vitro (84.6% vs. 79.6%).
  
  
  CONCLUSIONS
  Our results suggest that IVM of immature oocytes combined with TESE-ICSI is an option for couples with PCO and azoospermia. However, there are lower fertilization and good quality embryo rates achieved when TESE-ICSI was done with in vitro-matured oocytes. Additional studies are necessary to determine the role of this treatment combination.
authors.affiliation: Department of Obstetrics, MUHC Reproductive Center, and Gynecology, Montreal, Quebec, Canada. Electronic address: weon-young.son@muhc.mcgill.ca.
authors.forename: Weon-Young
authors.initials: WY
authors.lastname: Son
authors.forename: Jin-Tae
authors.initials: JT
authors.lastname: Chung
authors.forename: Sara
authors.initials: S
authors.lastname: Henderson
authors.forename: Shauna
authors.initials: S
authors.lastname: Reinblatt
authors.forename: William
authors.initials: W
authors.lastname: Buckett
authors.forename: Peter T K
authors.initials: PT
authors.lastname: Chan
authors.forename: Hananel
authors.initials: H
authors.lastname: Holzer
mesh_terms: D000328:Adult; D053713:Azoospermia / Q000150:complications* / Q000503:physiopathology; D006063:Chorionic Gonadotropin / Q000008:administration & dosage*; D004334:Drug Administration Schedule; D046149:Embryo Culture Techniques; D004624:Embryo Transfer; D047108:Embryonic Development; D005260:Female; D005298:Fertility; D005300:Fertility Agents, Female / Q000008:administration & dosage*; D006784:Hospitals, Teaching; D006801:Humans; D007246:Infertility / Q000209:etiology / Q000503:physiopathology / Q000628:therapy*; D008297:Male; D009865:Oocytes / Q000187:drug effects*; D010060:Ovulation / Q000187:drug effects*; D010062:Ovulation Induction / Q000379:methods*; D011085:Polycystic Ovary Syndrome / Q000150:complications* / Q000503:physiopathology; D011247:Pregnancy; D018873:Pregnancy Rate; D059285:Pregnancy, Twin; D012189:Retrospective Studies; D020554:Sperm Injections, Intracytoplasmic; D053625:Sperm Retrieval; D013997:Time Factors; D016896:Treatment Outcome
chemical_list: D006063:Chorionic Gonadotropin; D005300:Fertility Agents, Female
keywords: Azoospermia; immature oocytes; in vitro maturation; polycystic ovaries; testicular sperm extraction