title: A fraction rich in phenyl propanoids from L. divaricata aqueous extract is capable of inducing apoptosis, in relation to H₂O₂ modulation, on a murine lymphoma cell line.
abstract: Leukemia and lymphoma are a group of heterogeneous neoplastic disorder of white blood cells characterized by the uncontrolled proliferation and block in differentiation of hematopoietic cells. Nowadays, there is an interest in therapy with drugs of plant origin because conventional medicine can be inefficient or also results in side effects. Larrea divaricata Cav., is a plant widely distributed in Argentina that possess antiproliferative and antioxidant activities reported. Nordihydroguaiaretic acid (NDGA) was previously found in the plant and related to both antiproliferative and pro-proliferative actions on a lymphoma cell line. In order to demonstrate whether the presence of NDGA may be beneficial or not in the antiproliferative action of the aqueous extract, the extract of L. divaricata was submitted to a fractionation and fractions with and without NDGA were studied in a murine lymphocitic leukemia cell line (EL-4) proliferation. The effect of the most active fraction was studied in relation to H2O2 modulation and the synergistic action between compounds, found in fractions, was analyzed. The presence of NDGA was not a detonator for pro-proliferative action and its presence could be beneficial in low concentrations allowing a synergist antiproliferative action with other compounds.
authors.affiliation: Department of Pharmacology, IQUIMEFA-UBA-CONICET, Buenos Aires University, CABA, Buenos Aires, Argentina. renzo16martino@hotmail.com
authors.forename: Renzo
authors.initials: R
authors.lastname: Martino
authors.forename: Valeria
authors.initials: V
authors.lastname: Sülsen
authors.forename: Rosario
authors.initials: R
authors.lastname: Alonso
authors.forename: Claudia
authors.initials: C
authors.lastname: Anesini
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D015153:Blotting, Western; D002374:Catalase / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects*; D005434:Flow Cytometry; D006861:Hydrogen Peroxide / Q000378:metabolism; D062385:Hydroxybenzoates / Q000737:chemistry*; D032442:Larrea / Q000737:chemistry*; D008223:Lymphoma / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology*; D051379:Mice; D016877:Oxidants / Q000378:metabolism; D009195:Peroxidase / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology*; D011422:Propionates / Q000737:chemistry*; D013482:Superoxide Dismutase / Q000378:metabolism; D013481:Superoxides / Q000378:metabolism; D014407:Tumor Cells, Cultured
chemical_list: D062385:Hydroxybenzoates; D016877:Oxidants; D010936:Plant Extracts; D011422:Propionates; D013481:Superoxides; D006861:Hydrogen Peroxide; D002374:Catalase; D009195:Peroxidase; D013482:Superoxide Dismutase; C017616:phenolic acid
keywords: EL-4 lymphoma; Larrea divaricata; NDGA; Oxidative stress; Quercetin-3-methyl ether