title: Pathogen levels and clinical response to periodontal treatment in patients with Interleukin 8 haplotypes.
abstract: The aim of this study was to investigate the effect of non-surgical treatment of periodontitis on the levels of periodontopathogens and clinical parameters in patients with different genetic backgrounds produced by polymorphisms in the Interleukin ( IL8) gene. Thirty patients grouped according to IL8 ATC/TTC or AGT/TTC haplotypes were submitted to non-surgical periodontal treatment. Levels of Porphyromonas gingivalis, Tannerella forsythia, and Treponema denticola were determined in 240 subgingival plaque samples by qPCR. The association between IL8 haplotypes and the levels of periodontopathogens and clinical parameters was investigated by multilevel analysis accounting for the clustering of diseased sites analyzed within patients. It was observed that neither levels of periodontopathogens nor non-surgical treatment was associated with the IL8 haplotype. The clinical parameters after periodontal treatment were similar in diseased and healthy sites, independently of the IL8 haplotype. Nonetheless, in the same period, diseased sites of AGT/TTC patients harbored higher levels of P. gingivalis, T. denticola, T. forsythia, and red complex than those of ATC/TTC patients. However, the non-surgical periodontal therapy decreased the levels of these periodontopathogens and of the tested clinical parameters of diseased sites in both groups. Non-surgical therapy is equally effective in improving clinical parameters and decreasing the levels of periodontopathogens, independent of the genotype groups produced by the IL8 haplotype.
authors.affiliation: Department of Oral Diagnosis and Surgery, School of Dentistry at Araraquara, UNESP- Universidade Estadual Paulista, Araraquara, SP, Brazil.
authors.forename: Livia S
authors.initials: LS
authors.lastname: Finoti
authors.affiliation: Department of Oral Diagnosis and Surgery, School of Dentistry at Araraquara, UNESP- Universidade Estadual Paulista, Araraquara, SP, Brazil.
authors.forename: Sâmia C T
authors.initials: SC
authors.lastname: Corbi
authors.affiliation: Department of Oral Diagnosis and Surgery, School of Dentistry at Araraquara, UNESP- Universidade Estadual Paulista, Araraquara, SP, Brazil.
authors.forename: Giovana
authors.initials: G
authors.lastname: Anovazzi
authors.affiliation: Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil.
authors.forename: Silvia R L
authors.initials: SR
authors.lastname: Teixeira
authors.affiliation: Department of Physical Chemistry, Institute of Chemistry at Araraquara, UNESP- Universidade Estadual Paulista, Araraquara, SP, Brazil.
authors.forename: Marisa V
authors.initials: MV
authors.lastname: Capela
authors.affiliation: Department of Morphology, School of Dentistry at Araraquara, UNESP- Universidade Estadual Paulista, Araraquara, SP, Brazil.
authors.forename: Márcia H
authors.initials: MH
authors.lastname: Tanaka
authors.affiliation: Department of Oral Diagnosis and Surgery, School of Dentistry at Araraquara, UNESP- Universidade Estadual Paulista, Araraquara, SP, Brazil.
authors.forename: Yeon J
authors.initials: YJ
authors.lastname: Kim
authors.affiliation: Department of Oral Diagnosis and Surgery, School of Dentistry at Araraquara, UNESP- Universidade Estadual Paulista, Araraquara, SP, Brazil.
authors.forename: Silvana R P
authors.initials: SR
authors.lastname: Orrico
authors.affiliation: Department of Oral Diagnosis and Surgery, School of Dentistry at Araraquara, UNESP- Universidade Estadual Paulista, Araraquara, SP, Brazil.
authors.forename: Joni A
authors.initials: JA
authors.lastname: Cirelli
authors.affiliation: Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil.
authors.forename: Marcia P A
authors.initials: MP
authors.lastname: Mayer
authors.affiliation: Department of Morphology, School of Dentistry at Araraquara, UNESP- Universidade Estadual Paulista, Araraquara, SP, Brazil raquel@foar.unesp.br.
authors.forename: Raquel M
authors.initials: RM
authors.lastname: Scarel-Caminaga
mesh_terms: D058491:Bacterial Load; D003773:Dental Plaque / Q000382:microbiology; D020022:Genetic Predisposition to Disease; D006239:Haplotypes; D006801:Humans; D016209:Interleukin-8 / Q000235:genetics*; D010518:Periodontitis / Q000235:genetics* / Q000382:microbiology / Q000473:pathology / Q000628:therapy; D016966:Porphyromonas gingivalis / Q000302:isolation & purification*; D060888:Real-Time Polymerase Chain Reaction; D000070436:Tannerella forsythia / Q000302:isolation & purification*; D016896:Treatment Outcome; D048373:Treponema denticola / Q000302:isolation & purification*
chemical_list: C508606:CXCL8 protein, human; D016209:Interleukin-8
keywords: bacteria; chemokines; genetics; microbiology; periodontitis