title: Genome-wide identification and comparative expression analysis of NBS-LRR-encoding genes upon Colletotrichum gloeosporioides infection in two ecotypes of Fragaria vesca.
abstract: Anthracnose caused by Colletotrichum spp. is one of the most destructive diseases of cultivated strawberry (Fragaria×ananassa Duchesne) worldwide. The correlation between NBS-LRR genes, the largest class of known resistance genes, and strawberry anthracnose resistance has been elusive. BLAST search in NCBI identified 94 FvNBSs in the diploid genome of strawberry Fragaria vesca, with 67 of the TIR-NBS-LRR type. At least 36 FvNBSs were expressed, with 25% being non-coding genes. Two F. vesca ecotypes, HLJ and YW, showed great variations in both morphological and physiological responses upon C. gloeosporioides infection. qRT-PCR revealed that 5 of the 12 leaf-expressed FvNBSs displaying opposite transcription responses to C. gloeosporioides infection in two ecotypes. These results showed that the transcriptional responses of several FvNBSs were involved in the ecotype-specific responses to C. gloeosporioides in F. vesca. These FvNBSs hold potential in characterizing molecular components and developing novel markers associated with anthracnose resistance in strawberry.
authors.affiliation: Shanghai Key Laboratory of Protected Horticultural Technology, Forestry and Fruit Tree Research Institute, Shanghai Academy of Agricultural Sciences (SAAS), Shanghai 201403, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.forename: Qing-Yu
authors.initials: QY
authors.lastname: Zhang
authors.forename: Zhi-Hong
authors.initials: ZH
authors.lastname: Gao
authors.forename: Fei
authors.initials: F
authors.lastname: Wang
authors.forename: Ke
authors.initials: K
authors.lastname: Duan
authors.forename: Zheng-Wen
authors.initials: ZW
authors.lastname: Ye
authors.forename: Qing-Hua
authors.initials: QH
authors.lastname: Gao
mesh_terms: D002874:Chromosome Mapping; D020231:Colletotrichum / Q000502:physiology*; D060467:Disease Resistance / Q000235:genetics; D031985:Fragaria / Q000235:genetics* / Q000378:metabolism / Q000382:microbiology; D017343:Genes, Plant; D056426:Genetic Loci; D018745:Genome, Plant; D054884:Host-Pathogen Interactions; D008315:Malondialdehyde / Q000378:metabolism; D005810:Multigene Family; D010641:Phenotype; D010802:Phylogeny; D010935:Plant Diseases / Q000382:microbiology*; D018515:Plant Leaves / Q000235:genetics / Q000378:metabolism / Q000382:microbiology; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D017434:Protein Structure, Tertiary; D059467:Transcriptome
chemical_list: D010940:Plant Proteins; D008315:Malondialdehyde
keywords: Colletotrichum gloeosporioides; Fragaria vesca; Genetic variation; LG; NBS–LRR; R genes; RGA; Resistance gene; Resistance genes; TIR Toll and mammalian Interleukin-1 receptor-like; TNL TIR–NBS–LRR; days post inoculation; dpi; hours post inoculation; hpi; linkage group; nucleotide-binding site and leucine-rich repeat; qPCR; qRT-PCR; quantitative reverse-transcription PCR; resistance gene analogs