title: T85C polymorphisms of the dihydropyrimidine dehydrogenase gene detected in gastric cancer tissues by high-resolution melting curve analysis.
abstract: OBJECTIVE
  Dihydropyrimidine dehydrogenase is a key enzyme acting on the metabolic pathway of medications for gastric cancer. High-resolution melting curve technology, which was developed recently, can distinguish the wild-type dihydropyrimidine dehydrogenase gene from multiple polymorphisms by fluorescent quantitative polymerase chain reaction products in a direct and effective manner.
  
  
  METHODS
  T85C polymorphisms of dihydropyrimidine dehydrogenase in the peripheral blood of 112 Chinese gastric cancer patients were detected by real-time polymerase chain reaction combined with high-resolution melting curve technology. Primer design, along with the reaction system and conditions, was optimized based on the GenBank sequence.
  
  
  RESULTS
  Seventy nine cases of wild-type (TT, [70.5%]), 29 cases of heterozygous (TC, [25.9%]), and 4 cases of homozygous mutant (CC, [3.6%]) were observed. The result was completely consistent with the results of the sequencing.
  
  
  CONCLUSIONS
  Real-time polymerase chain reaction combined with high-resolution melting curve technology is a rapid, simple, reliable, direct-viewing, and convenient method for the detection and screening of polymorphisms.
authors.affiliation: Department of Medical Oncology, The First Affiliated Hospital, College of Medicine, Zhejiang University, Zhejiang, China
authors.forename: Weijia
authors.initials: W
authors.lastname: Fang
authors.forename: Nong
authors.initials: N
authors.lastname: Xu
authors.forename: Dazhi
authors.initials: D
authors.lastname: Jin
authors.forename: Yu
authors.initials: Y
authors.lastname: Chen
authors.forename: Xiaogang
authors.initials: X
authors.lastname: Chen
authors.forename: Yi
authors.initials: Y
authors.lastname: Zheng
authors.forename: Hong
authors.initials: H
authors.lastname: Shen
authors.forename: Ying
authors.initials: Y
authors.lastname: Yuan
authors.forename: Shusen
authors.initials: S
authors.lastname: Zheng
mesh_terms: D000328:Adult; D044466:Asian People / Q000235:genetics; D042943:Dihydrouracil Dehydrogenase (NADP) / Q000235:genetics*; D005260:Female; D020022:Genetic Predisposition to Disease / Q000235:genetics; D005820:Genetic Testing / Q000379:methods* / Q000592:standards*; D006579:Heterozygote; D006720:Homozygote; D006801:Humans; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods* / Q000592:standards*; D015203:Reproducibility of Results; D013274:Stomach Neoplasms / Q000235:genetics*
chemical_list: D042943:Dihydrouracil Dehydrogenase (NADP)