title: Role of the mannose receptor in phagocytosis of Enterococcus faecalis strain EC-12 by antigen-presenting cells.
abstract: The aim of this study was to clarify the phagocytic mechanisms of a heat-killed cell preparation of Enterococcus faecalis strain EC-12 (EC-12) by antigen-presenting cells (APCs). Fluorescein isothiocyanate (FITC)-labeled EC-12 was cocultured with peritoneal macrophage and the amount of EC-12 phagocytosed by peritoneal macrophages was measured using a microplate fluorometer. Peritoneal macrophages from toll-like receptor (TLR)2-, TLR7-, and MyD88-deficient knockout (KO) mice exhibited similar levels of EC-12 phagocytosis to those from wild-type mice. Similarly, dectin-1 neutralization of peritoneal macrophages had no effect on EC-12 phagocytosis. However, blockade of the mannose receptor (MR) significantly decreased the amount of EC-12 phagocytosed by peritoneal macrophages; the same effect was observed in bone marrow-derived macrophages and dendritic cells. Our findings suggest that MR plays a major role in EC-12 phagocytosis by the APCs.
authors.affiliation: Laboratory of Animal Science, Kyoto Prefectural University, 1-5 Shimogamohangityou, Sakyo-ku, Kyoto, Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Tsuruta
authors.forename: Ryo
authors.initials: R
authors.lastname: Inoue
authors.forename: Takayuki
authors.initials: T
authors.lastname: Nagino
authors.forename: Ryoichiro
authors.initials: R
authors.lastname: Nishibayashi
authors.forename: Yuko
authors.initials: Y
authors.lastname: Makioka
authors.forename: Kazunari
authors.initials: K
authors.lastname: Ushida
mesh_terms: D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology* / Q000382:microbiology*; D003713:Dendritic Cells / Q000276:immunology / Q000382:microbiology; D013293:Enterococcus faecalis / Q000276:immunology*; D037181:Lectins, C-Type / Q000172:deficiency / Q000378:metabolism*; D008264:Macrophages / Q000276:immunology* / Q000382:microbiology*; D000090323:Mannose Receptor; D037241:Mannose-Binding Lectins / Q000172:deficiency / Q000378:metabolism*; D051379:Mice; D018345:Mice, Knockout; D010587:Phagocytosis; D011956:Receptors, Cell Surface / Q000172:deficiency / Q000378:metabolism*
chemical_list: D037181:Lectins, C-Type; D000090323:Mannose Receptor; D037241:Mannose-Binding Lectins; D011956:Receptors, Cell Surface
keywords: Antigen-presenting cell; EC-12; LAB; macrophage; mannose receptor; phagocytosis