title: Immunogenicity and safety of H1N1 influenza hemagglutinin protein expressed in a baculovirus system.
abstract: Although most influenza vaccines are produced in eggs, new types of vaccines must be developed. In this study, the immunogenicity and safety of a baculovirus-expressed hemagglutinin (HA) of H1N1 influenza virus (Korea/01/2009; designated "HA-Bac-K") was compared with those of a commercially available baculovirus-expressed HA (designated "HA-Bac-C") and an Escherichia coli-expressed HA (designated "HA-E. Coli-K"). HA-Bac-K succeeded in inducing hemagglutination inhibition and neutralization antibodies in mouse and ferret models. The different immunogenicities observed may be attributable to the different expression systems and purification protocols used. Our work suggests that HA expressed in a baculovirus system is an effective and safe candidate influenza vaccine.
authors.affiliation: Department of Biotechnology, Catholic University of Korea, Bucheon, 420-743.
authors.forename: Ha-Na
authors.initials: HN
authors.lastname: Na
authors.forename: Kyung Hyun
authors.initials: KH
authors.lastname: Kim
authors.forename: Man Ki
authors.initials: MK
authors.lastname: Song
authors.forename: Hye-Lim
authors.initials: HL
authors.lastname: Park
authors.forename: Eun-Young
authors.initials: EY
authors.lastname: Lee
authors.forename: Seung-Hyun
authors.initials: SH
authors.lastname: Shim
authors.forename: Sooho
authors.initials: S
authors.lastname: Park
authors.forename: Jae-Hwan
authors.initials: JH
authors.lastname: Nam
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D016367:Baculoviridae / Q000235:genetics* / Q000378:metabolism; D005260:Female; D005289:Ferrets; D015870:Gene Expression; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000009:adverse effects / Q000235:genetics* / Q000276:immunology*; D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype / Q000235:genetics / Q000276:immunology*; D007252:Influenza Vaccines / Q000008:administration & dosage / Q000009:adverse effects / Q000235:genetics / Q000276:immunology*; D007251:Influenza, Human / Q000276:immunology* / Q000517:prevention & control / Q000821:virology; D051379:Mice
chemical_list: D000914:Antibodies, Viral; D019267:Hemagglutinin Glycoproteins, Influenza Virus; D007252:Influenza Vaccines
keywords: hemagglutinin; influenza virus; vaccine