title: Proteomics analysis of human pericardial fluid.
abstract: Pericardial fluid (PF) is considered as a biochemical window of heart. To date, there have been limited attempts to perform an in-depth analysis of the PF proteome. In this study, an SDS-PAGE-LC-MS/MS platform was utilized to explore depleted PF, which showed great coverage of low-abundant proteins. In total, 1007 nonredundant proteins were identified with at least two peptides. This is the first comprehensive analysis of human PF proteome and provides a foundation for further application of PF in cardiovascular research. The data have been deposited to the ProteomeXchange with identifier PXD000194.
authors.affiliation: Department of Thoracic and Cardiovascular Surgery, Nanjing First Hospital Affiliated to Nanjing Medical University, Nanjing, P. R. China.
authors.forename: Fei
authors.initials: F
authors.lastname: Xiang
authors.forename: Xuejiang
authors.initials: X
authors.lastname: Guo
authors.forename: Wen
authors.initials: W
authors.lastname: Chen
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.forename: Tao
authors.initials: T
authors.lastname: Zhou
authors.forename: Fuhua
authors.initials: F
authors.lastname: Huang
authors.forename: Changchun
authors.initials: C
authors.lastname: Cao
authors.forename: Xin
authors.initials: X
authors.lastname: Chen
mesh_terms: D000293:Adolescent; D000328:Adult; D002853:Chromatography, Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D006321:Heart; D006801:Humans; D010490:Pericardial Effusion / Q000378:metabolism*; D011506:Proteins / Q000032:analysis*; D020543:Proteome / Q000032:analysis*; D040901:Proteomics; D053719:Tandem Mass Spectrometry; D055815:Young Adult
chemical_list: D011506:Proteins; D020543:Proteome
keywords: Heart; Pericardial fluid; Systems biology