title: mazEF-mediated programmed cell death in bacteria: "what is this?".
abstract: Toxin-antitoxin (TA) systems consist of a bicistronic operon, encoding a toxin and an antitoxin. They are widely distributed in the prokaryotic kingdom, often in multiple numbers. TAs are implicated in contradicting phenomena of persistence and programmed cell death (PCD) in bacteria. mazEF TA system, one of the widely distributed type II toxin-antitoxin systems, is particularly implicated in PCD of Escherichia coli. Nutrient starvation, antibiotic stress, heat shock, DNA damage and other kinds of stresses are shown to elicit mazEF-mediated-PCD. ppGpp and extracellular death factor play a central role in regulating mazEF-mediated PCD. The activation of mazEF system is achieved through inhibition of transcription or translation of mazEF loci. Upon activation, MazF cleaves RNA in a ribosome-independent fashion and subsequent processes result in cell death. It is hypothesized that PCD aids in perseverance of the population during stress; the surviving minority of the cells can scavenge the nutrients released by the dead cells, a kind of "nutritional-altruism." Issues regarding the strains, reproducibility of experimental results and ecological plausibility necessitate speculation. We review the molecular mechanisms of the activation of mazEF TA system, the consequences leading to cell death and the pros and cons of the altruism hypothesis from an ecological perspective.
authors.affiliation: School of Chemical and Biotechnology, SASTRA University , Thanjavur , India and.
authors.forename: Bhaskar Chandra Mohan
authors.initials: BC
authors.lastname: Ramisetty
authors.forename: Bhargavi
authors.initials: B
authors.lastname: Natarajan
authors.forename: Ramachandran Sarojini
authors.initials: RS
authors.lastname: Santhosh
mesh_terms: D018407:Bacterial Physiological Phenomena; D016923:Cell Death; D004268:DNA-Binding Proteins; D004722:Endoribonucleases; D004926:Escherichia coli / Q000502:physiology; D029968:Escherichia coli Proteins; D050296:Microbial Viability; D013312:Stress, Physiological
chemical_list: D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; C422954:MazE protein, E coli; C422955:MazF protein, E coli; D004722:Endoribonucleases
keywords: Bacterial stress physiology; Escherichia coli; extra cellular death factor; persistence; toxin–antitoxin systems