title: Paenibacillus marinisediminis sp. nov., a bacterium isolated from marine sediment.
abstract: A Gram-negative, nonmotile, endospore-forming, rod-shaped bacterial strain LHW35(T), which belonged to the genus Paenibacillus, was isolated from marine sediment collected from the south coast of the Republic of Korea. A phylogenetic analysis of 16S rRNA gene sequences indicated that strain LHW35(T) was most closely related to Paenibacillus taiwanensis G-soil-2-3(T) (97.2% similarity). The optimal growth conditions for strain LHW35(T) were 37°C, pH 6.0, and 0% (w/v) NaCl. The main isoprenoid quinone was menaquinone-7 (MK-7) and the major polyamine was spermidine. The diamino acid present in the cell-wall peptidoglycan was meso-diaminopimelic acid. The major fatty acids were anteiso-C15:0 and C16:0. The polar lipids were phosphatidylethanolamine, phosphatidylglycerol, unidentified amino-hospholipids, unidentified phospholipids, and unidentified polar lipids. A DNA-DNA hybridization experiment using the type strain of P. taiwanensis indicated <40% relatedness. The DNA G+C content was 45.0 mol%. Based on these phylogenetic, genomic, and phenotypic analyses, strain LHW35(T) should be classified as a novel species within the genus Paenibacillus, for which the name Paenibacillus marinisediminis sp. nov. is proposed. The type strain is LHW35(T) (=KACC 16317(T) =JCM 17886(T)).
authors.affiliation: Department of Life and Nanopharmaceutical Sciences and Department of Biology, Kyung Hee University, Seoul 130-701, Republic of Korea.
authors.forename: Hae-Won
authors.initials: HW
authors.lastname: Lee
authors.forename: Seong Woon
authors.initials: SW
authors.lastname: Roh
authors.forename: Kyung June
authors.initials: KJ
authors.lastname: Yim
authors.forename: Na-Ri
authors.initials: NR
authors.lastname: Shin
authors.forename: Jina
authors.initials: J
authors.lastname: Lee
authors.forename: Tae Woong
authors.initials: TW
authors.lastname: Whon
authors.forename: Joon Yong
authors.initials: JY
authors.lastname: Kim
authors.forename: Dong-Wook
authors.initials: DW
authors.lastname: Hyun
authors.forename: Daekyung
authors.initials: D
authors.lastname: Kim
authors.forename: Jin-Woo
authors.initials: JW
authors.lastname: Bae
mesh_terms: D001482:Base Composition / Q000235:genetics; D003960:Diaminopimelic Acid / Q000378:metabolism; D019015:Geologic Sediments / Q000382:microbiology*; D056507:Paenibacillus / Q000145:classification / Q000235:genetics* / Q000378:metabolism*; D010714:Phosphatidylethanolamines / Q000378:metabolism; D010715:Phosphatidylglycerols / Q000378:metabolism; D010743:Phospholipids / Q000378:metabolism; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013095:Spermidine / Q000378:metabolism
chemical_list: D010714:Phosphatidylethanolamines; D010715:Phosphatidylglycerols; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; C483858:phosphatidylethanolamine; D003960:Diaminopimelic Acid; D013095:Spermidine