title: Direct ethanol fermentation from lignocellulosic biomass by Antarctic basidiomycetous yeast Mrakia blollopis under a low temperature condition.
abstract: Antarctic basidiomycetous yeast Mrakia blollopis SK-4 has unique fermentability for various sugars under a low temperature condition. Hence, this yeast was used for ethanol fermentation from glucose and also for direct ethanol fermentation (DEF) from cellulosic biomass without/with Tween 80 at 10°C. Maximally, 48.2 g/l ethanol was formed from 12% (w/v) glucose. DEF converted filter paper, Japanese cedar and Eucalyptus to 12.2 g/l, 12.5 g/l and 7.2 g/l ethanol, respectively. In the presence of 1% (v/v) Tween 80, ethanol concentration increased by about 1.1-1.6-fold compared to that without Tween 80. This is the first report on DEF using cryophilic fungi under a low temperature condition. We consider that M. blollopis SK-4 has a good potential for ethanol fermentation in cold environments.
authors.affiliation: Biomass Refinery Research Center (BRRC), National Institute of Advanced Industrial Science and Technology (AIST), 3-11-32, Kagamiyama, Higashihiroshima, Hirosima 739-0046, Japan.
authors.forename: Masaharu
authors.initials: M
authors.lastname: Tsuji
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Goshima
authors.forename: Akinori
authors.initials: A
authors.lastname: Matsushika
authors.forename: Sakae
authors.initials: S
authors.lastname: Kudoh
authors.forename: Tamotsu
authors.initials: T
authors.lastname: Hoshino
mesh_terms: D000864:Antarctic Regions; D001487:Basidiomycota / Q000378:metabolism*; D018533:Biomass; D003080:Cold Temperature; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation; D008031:Lignin / Q000378:metabolism*
chemical_list: C036909:lignocellulose; D000431:Ethanol; D008031:Lignin
keywords: Antarctica; Cryophilic yeast; Direct ethanol fermentation; Ethanol fermentation; Lignocellulosic biomass