title: A novel glucose biosensor platform based on Ag@AuNPs modified graphene oxide nanocomposite and SERS application.
abstract: This study represents a novel template demonstration of a glucose biosensor based on mercaptophenyl boronic acid (MBA) terminated Ag@AuNPs/graphene oxide (Ag@AuNPs-GO) nanomaterials. The nanocomposites were characterized by transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS), and X-ray diffraction (XRD) method. The TEM image shows that Ag@AuNPs in the nanocomposite is in the range of diameters of 10-20 nm. The nanocomposite was used for the determination of glucose through the complexation between boronic acid and diol groups of glucose. Thus, a novel glucose biosensor was further fabricated by immobilizing glucose oxidase (GOD) into MBA terminated Ag@AuNPs-GO nanocomposite film (MBA-Ag@AuNPs-GO). The linearity range of glucose was obtained as 2-6mM with detection limit of 0.33 mM. The developed biosensor was also applied successfully for the determination of glucose in blood samples. The concentration value of glucose in blood samples was calculated to be 1.97±0.002 mM from measurements repeated for six times.
authors.affiliation: Department of Chemistry, Indian Institute of Technology Roorkee, Roorkee, India. vinodfcy@iitr.ernet.in
authors.forename: Vinod Kumar
authors.initials: VK
authors.lastname: Gupta
authors.forename: Necip
authors.initials: N
authors.lastname: Atar
authors.forename: Mehmet Lütfi
authors.initials: ML
authors.lastname: Yola
authors.forename: Merve
authors.initials: M
authors.lastname: Eryılmaz
authors.forename: Hilal
authors.initials: H
authors.lastname: Torul
authors.forename: Uğur
authors.initials: U
authors.lastname: Tamer
authors.forename: Ismail Hakkı
authors.initials: IH
authors.lastname: Boyacı
authors.forename: Zafer
authors.initials: Z
authors.lastname: Ustündağ
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D001786:Blood Glucose / Q000032:analysis; D001897:Boronic Acids / Q000737:chemistry; D004800:Enzymes, Immobilized / Q000378:metabolism*; D005949:Glucose Oxidase / Q000378:metabolism*; D006046:Gold / Q000737:chemistry; D006108:Graphite / Q000737:chemistry; D006801:Humans; D053761:Nanocomposites / Q000737:chemistry*; D056951:Photoelectron Spectroscopy; D012834:Silver / Q000737:chemistry; D013059:Spectrum Analysis, Raman; D013438:Sulfhydryl Compounds / Q000737:chemistry
chemical_list: D001786:Blood Glucose; D001897:Boronic Acids; D004800:Enzymes, Immobilized; D013438:Sulfhydryl Compounds; D012834:Silver; D006046:Gold; D006108:Graphite; D005949:Glucose Oxidase
keywords: Ag@AuNPs; Characterization; Glucose biosensor; Graphene oxide; SERS