title: Clinical study using adipose-derived mesenchymal-like stem cells in acute myocardial infarction and heart failure.
abstract: Adipose tissue represents an abundant, accessible source of regenerative cells that can be easily obtained in sufficient amount for therapy. Adipose-derived regenerative cells (ADRC) are comprised of leukocytes, smooth muscles, endothelial cells, and mesenchymal stem cells. In contrast to bone-marrow-derived MSC, the abundance of adipose tissue in patients and the higher frequency per unit mass of regenerative cells allow for the isolation of cells in therapeutic meaningful amounts in less than 2h after donor tissue acquisition.Harvest of adipose tissue can thus follow primary PCI, allowing efficient treatment within 24h. This obviates the need for extensive cell culturing in GMP clean room facilities and makes ADSCs a promising and practical autologous cell source. In the following chapter, we will describe the liposuction procedure for stem cell harvest, two cell delivery techniques, and pressure/volume loop analysis for the follow-up of our patients enrolled in the clinical studies.
authors.affiliation: Molecular Cardiology Laboratory & Intervention Cardiology, Thoraxcenter Rotterdam, Rotterdam, The Netherlands.
authors.forename: Ilia Alexander
authors.initials: IA
authors.lastname: Panfilov
authors.forename: Renate
authors.initials: R
authors.lastname: de Jong
authors.forename: Shin-Ichiro
authors.initials: S
authors.lastname: Takashima
authors.forename: Henricus J
authors.initials: HJ
authors.lastname: Duckers
mesh_terms: D000208:Acute Disease; D000273:Adipose Tissue / Q000637:transplantation*; D002454:Cell Differentiation; D002469:Cell Separation / Q000379:methods*; D002478:Cells, Cultured; D002986:Clinical Trials as Topic; D006333:Heart Failure / Q000628:therapy*; D006801:Humans; D015187:Lipectomy / Q000379:methods*; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D009203:Myocardial Infarction / Q000628:therapy*