title: [Effect of BCL11A gene on transcription of γ-globin gene].
abstract: This study was aimed to explore the effect of BCL11A gene on transcription of γ-globin gene in K562 cells. B-cell lymphoma/leukemia 11A (BCL11A) gene was silenced by small interfering RNA (siRNA) expression vectors in K562 cells (human erythroblastic leukemia cell line). Gamma-globin mRNA level in K562 cells was determined by RT-PCR. Association between the BCL11A gene and γ-globin gene transcription was explored by comparison of mRNA levels. The results indicated that the silence rate of the BCL11A gene in K562 cells by 4 siRNA expression vectors was 49.7%, 55.4%, 78.2%, and 84.1%, respectively. The siRNA expression vector with 84.1% silence rate was transfected into K562 cells, transcription level of γ-globin mRNA in K562 cells transfected with siRNA expression vector increased 2.4 times as compared with control K562 cells. It is concluded that level of γ-globin mRNA increases when the BCL11A gene is silenced. It indicates that the BCL11A gene may be a negative regulator for γ-globin gene expression.
authors.affiliation: Department of Clinical Examination, Bao'an District People Hospital, Shenzhen, Guangdong Province, China. bayysun@sina.cn
authors.forename: Shun-Chang
authors.initials: SC
authors.lastname: Sun
authors.forename: Zhi-Ming
authors.initials: ZM
authors.lastname: Zhou
authors.forename: Chuan-Qing
authors.initials: CQ
authors.lastname: Tu
authors.forename: Yun-Sheng
authors.initials: YS
authors.lastname: Peng
authors.forename: Hui-Wen
authors.initials: HW
authors.lastname: Song
mesh_terms: D002352:Carrier Proteins / Q000235:genetics*; D015973:Gene Expression Regulation, Leukemic; D005809:Genes, Regulator; D005822:Genetic Vectors; D006801:Humans; D020014:K562 Cells; D009687:Nuclear Proteins / Q000235:genetics*; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics; D012097:Repressor Proteins; D014158:Transcription, Genetic; D014162:Transfection; D055545:gamma-Globins / Q000235:genetics*
chemical_list: C486563:BCL11A protein, human; D002352:Carrier Proteins; D009687:Nuclear Proteins; D034741:RNA, Small Interfering; D012097:Repressor Proteins; D055545:gamma-Globins