title: [(-)-Epigallocatechin gallate regulates expression of apoptotic genes and protects cultured human lens epithelial cells under hyperglycemia].
abstract: (-)-Epigallocatechin gallate (EGCG), the most abundant component in green tea, has a potent anti-apoptotic activity. The purpose of this study was to investigate the protective effects of EGCG and their molecular mechanisms on high glucose-induced apoptosis of human lens epithelial cells (HLEB-3). HLEB-3 cells were exposed to various concentrations of glucose and EGCG. Cell death was assessed by MTT assay and flow cytometry using annexin V and propidium iodide. The expression of the Bcl-2 family, c-fos, c-myc and p53 was measured by real-time PCR. EGCG decreased the Bcl-2/Bax expression stimulated by a high glucose. Moreover, EGCG suppressed the high glucose-induced expression of c-fos, c-myc and p53. These findings suggest that EGCG protects HLEB-3 cells from high glucose-induced apoptosis by regulating the gene expression of the Bcl-2 family, c-fos, c-myc and p53. Thus, EGCG may have a potential protective effect against diabetic cataract formation.
authors.forename: Panpan
authors.initials: P
authors.lastname: Ye
authors.forename: Kana
authors.initials: K
authors.lastname: Lin
authors.forename: Zhaochun
authors.initials: Z
authors.lastname: Li
authors.forename: Jian
authors.initials: J
authors.lastname: Liu
authors.forename: Ke
authors.initials: K
authors.lastname: Yao
authors.forename: Wen
authors.initials: W
authors.lastname: Xu
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D028241:Camellia sinensis / Q000737:chemistry; D002386:Cataract / Q000150:complications / Q000188:drug therapy / Q000378:metabolism; D002392:Catechin / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology; D048909:Diabetes Complications / Q000188:drug therapy / Q000378:metabolism; D003920:Diabetes Mellitus; D004847:Epithelial Cells / Q000166:cytology / Q000187:drug effects; D005786:Gene Expression Regulation / Q000187:drug effects*; D005947:Glucose / Q000494:pharmacology; D006801:Humans; D006943:Hyperglycemia; D007903:Lens Capsule, Crystalline / Q000166:cytology / Q000187:drug effects / Q000378:metabolism
chemical_list: D002392:Catechin; C045651:epigallocatechin gallate; D005947:Glucose