title: Paenalcaligenes hermetiae sp. nov., isolated from the larval gut of Hermetia illucens (Diptera: Stratiomyidae), and emended description of the genus Paenalcaligenes.
abstract: A novel Gram-stain-negative, facultatively anaerobic, non-motile and short rod-shaped bacterium, strain KBL009(T), was isolated from the larval gut of Hermetia illucens. Strain KBL009(T) grew optimally at 37 °C, at pH 6.0 and with 1-2 % (w/v) NaCl. The 16S rRNA gene sequence of strain KBL009(T) showed 97.6 % similarity to that of Paenalcaligenes hominis CCUG 53761A(T) indicating its classification with the genus Paenalcaligenes. The major fatty acids were cyclo-C17 : 0, C16 : 0 and summed feature 2 (comprising C14 : 0 3-OH/iso-C16 : 1). The respiratory quinones were ubiquinone-8 (Q-8), predominating, and a minor amount of Q-7. The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, one unknown aminolipid and five unknown polar lipids. The polyamine pattern contained predominantly putrescine and relatively high amounts of spermidine. The betaproteobacterial-specific 2-hydroxyputrescine could only be detected in trace amounts. The G+C content of genomic DNA was 56.1 mol%. Results from DNA-DNA hybridization with P. hominis KCTC 23583(T) unambiguously demonstrated that strain KBL009(T) represents a novel species in the genus Paenalcaligenes. Based on phenotypic, genotypic and phylogenetic characterization, the novel species Paenalcaligenes hermetiae sp. nov. is proposed. The type strain is KBL009(T) ( = KACC 16840(T) = JCM 18423(T)). An emended description of the genus Paenalcaligenes is also provided.
authors.affiliation: Department of Biology, Kyung Hee University, 130-701 Seoul, Republic of Korea.
authors.forename: Youn Yeop
authors.initials: YY
authors.lastname: Lee
authors.affiliation: Department of Biology, Kyung Hee University, 130-701 Seoul, Republic of Korea.
authors.forename: Jae Kook
authors.initials: JK
authors.lastname: Lee
authors.affiliation: Insect Resources Division, Department of Agricultural Biology, National Academy of Agricultural Science and Technology, Rural Development Administration, 441-853 Suwon, Republic of Korea.
authors.forename: Kwan Ho
authors.initials: KH
authors.lastname: Park
authors.affiliation: Department of Biology, Kyung Hee University, 130-701 Seoul, Republic of Korea.
authors.forename: Seo-Yeon
authors.initials: SY
authors.lastname: Kim
authors.affiliation: Jeju Center, Korea Basic Science Institute, 690-140 Jeju, Republic of Korea.
authors.forename: Seong Woon
authors.initials: SW
authors.lastname: Roh
authors.affiliation: Insect Resources Division, Department of Agricultural Biology, National Academy of Agricultural Science and Technology, Rural Development Administration, 441-853 Suwon, Republic of Korea.
authors.forename: Sang-Beom
authors.initials: SB
authors.lastname: Lee
authors.affiliation: Insect Resources Division, Department of Agricultural Biology, National Academy of Agricultural Science and Technology, Rural Development Administration, 441-853 Suwon, Republic of Korea.
authors.forename: Youngcheol
authors.initials: Y
authors.lastname: Choi
authors.affiliation: Department of Life and Nanopharmaceutical Sciences, Kyung Hee University, 130-701 Seoul, Republic of Korea.|Department of Biology, Kyung Hee University, 130-701 Seoul, Republic of Korea.
authors.forename: Sung-Jae
authors.initials: SJ
authors.lastname: Lee
mesh_terms: D042401:Alcaligenaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D000818:Animals; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D004175:Diptera / Q000382:microbiology*; D005227:Fatty Acids / Q000737:chemistry; D041981:Gastrointestinal Tract / Q000382:microbiology*; D007814:Larva / Q000382:microbiology; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010743:Phospholipids / Q000235:genetics; D010802:Phylogeny; D011073:Polyamines / Q000737:chemistry; D011700:Putrescine / Q000031:analogs & derivatives; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D017422:Sequence Analysis, DNA; D014451:Ubiquinone / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D011073:Polyamines; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; C002952:2-hydroxyputrescine; C030778:ubiquinone 8; D011700:Putrescine