title: Temperature: the "ignored" factor at the NanoBio interface.
abstract: Upon incorporation of nanoparticles (NPs) into the body, they are exposed to biological fluids, and their interaction with the dissolved biomolecules leads to the formation of the so-called protein corona on the surface of the NPs. The composition of the corona plays a crucial role in the biological fate of the NPs. While the effects of various physicochemical parameters on the composition of the corona have been explored in depth, the role of temperature upon its formation has received much less attention. In this work, we have probed the effect of temperature on the protein composition on the surface of a set of NPs with various surface chemistries and electric charges. Our results indicate that the degree of protein coverage and the composition of the adsorbed proteins on the NPs' surface depend on the temperature at which the protein corona is formed. Also, the uptake of NPs is affected by the temperature. Temperature is, thus, an important parameter that needs to be carefully controlled in quantitative studies of bionano interactions.
authors.affiliation: Nanotechnology Research Center and Department of Nanotechnology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran. Mahmoudi-M@TUMS.ac.ir
authors.forename: Morteza
authors.initials: M
authors.lastname: Mahmoudi
authors.forename: Abuelmagd M
authors.initials: AM
authors.lastname: Abdelmonem
authors.forename: Shahed
authors.initials: S
authors.lastname: Behzadi
authors.forename: Joachim H
authors.initials: JH
authors.lastname: Clement
authors.forename: Silvio
authors.initials: S
authors.lastname: Dutz
authors.forename: Mohammad R
authors.initials: MR
authors.lastname: Ejtehadi
authors.forename: Raimo
authors.initials: R
authors.lastname: Hartmann
authors.forename: Karsten
authors.initials: K
authors.lastname: Kantner
authors.forename: Uwe
authors.initials: U
authors.lastname: Linne
authors.forename: Pauline
authors.initials: P
authors.lastname: Maffre
authors.forename: Scott
authors.initials: S
authors.lastname: Metzler
authors.forename: Mojhgan K
authors.initials: MK
authors.lastname: Moghadam
authors.forename: Christian
authors.initials: C
authors.lastname: Pfeiffer
authors.forename: Meisam
authors.initials: M
authors.lastname: Rezaei
authors.forename: Pilar
authors.initials: P
authors.lastname: Ruiz-Lozano
authors.forename: Vahid
authors.initials: V
authors.lastname: Serpooshan
authors.forename: Mohammad A
authors.initials: MA
authors.lastname: Shokrgozar
authors.forename: G Ulrich
authors.initials: GU
authors.lastname: Nienhaus
authors.forename: Wolfgang J
authors.initials: WJ
authors.lastname: Parak
mesh_terms: D000327:Adsorption; D001709:Biotechnology; D003102:Colloids / Q000737:chemistry; D006367:HeLa Cells; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008280:Magnetics; D013058:Mass Spectrometry; D018613:Microscopy, Confocal; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D011108:Polymers / Q000737:chemistry; D011485:Protein Binding; D011506:Proteins / Q000737:chemistry; D012709:Serum Albumin / Q000737:chemistry; D013499:Surface Properties; D013696:Temperature
chemical_list: D003102:Colloids; D011108:Polymers; D011506:Proteins; D012709:Serum Albumin