title: Fluorescent nanoparticle delivered dsRNA toward genetic control of insect pests.
abstract: A fluorescent cationic core-shell nanoparticle efficiently enters into cells with high transfection efficacy. A FNP/CHT10-dsRNA complex is orally fed to insect pests and knocks down a midgut-specific chitinase gene of the Asian corn borer, which leads to death. This is the first report on the genetic control of insect pests through a non-viral gene delivery system to knock down key developmental gene expression.
authors.affiliation: Department of Entomology, China Agricultural University, 100193 Beijing, China.
authors.forename: Bicheng
authors.initials: B
authors.lastname: He
authors.forename: Yuan
authors.initials: Y
authors.lastname: Chu
authors.forename: Meizhen
authors.initials: M
authors.lastname: Yin
authors.forename: Klaus
authors.initials: K
authors.lastname: Müllen
authors.forename: Chunju
authors.initials: C
authors.lastname: An
authors.forename: Jie
authors.initials: J
authors.lastname: Shen
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004330:Drosophila / Q000166:cytology / Q000187:drug effects* / Q000235:genetics; D005456:Fluorescent Dyes / Q000737:chemistry*; D005822:Genetic Vectors / Q000737:chemistry*; D007300:Insect Control; D007306:Insecticides / Q000494:pharmacology*; D015394:Molecular Structure; D053758:Nanoparticles / Q000737:chemistry*; D012330:RNA, Double-Stranded / Q000737:chemistry* / Q000235:genetics; D013329:Structure-Activity Relationship
chemical_list: D005456:Fluorescent Dyes; D007306:Insecticides; D012330:RNA, Double-Stranded
keywords: dsRNAi; fluorescence; gene carrier; insect; nanoparticle