title: Identification of perivascular mesenchymal stromal/stem cells by flow cytometry.
abstract: Mesenchymal stem/stromal cells (MSCs) are adult multipotent progenitors of great promise for cell therapy. MSCs can mediate tissue regeneration, immunomodulation, and hematopoiesis support. Despite the unique properties of MSCs and their broad range of potential clinical applications, the very nature of these cells has been uncertain. Furthermore, MSCs are heterogeneous and only defined subpopulations of these are endowed with the particular abilities to sustain hematopoietic stem cells, regulate immune responses, or differentiate into mesodermal cell lineages. It is becoming evident that current criteria used to define cultured polyclonal MSCs (expression of nonspecific markers and in vitro mesodermal differentiation) are not sufficient to fully understand and exploit the potential of these cells. Here, we describe how flow cytometry has been used to reveal a perivascular origin of MSCs. As a result, the prospective purification of MSCs and specialized subsets thereof is now possible, and the clinical use of purified autologous MSCs is now within reach.
authors.affiliation: UCLA-Orthopaedic Hospital Department of Orthopaedic Surgery, The Orthopaedic Hospital Research Center, Los Angeles, CA, USA.
authors.forename: Mirko
authors.initials: M
authors.lastname: Corselli
authors.forename: Mihaela
authors.initials: M
authors.lastname: Crisan
authors.forename: Iain R
authors.initials: IR
authors.lastname: Murray
authors.forename: Christopher C
authors.initials: CC
authors.lastname: West
authors.forename: Jessica
authors.initials: J
authors.lastname: Scholes
authors.forename: Felicia
authors.initials: F
authors.lastname: Codrea
authors.forename: Nusrat
authors.initials: N
authors.lastname: Khan
authors.forename: Bruno
authors.initials: B
authors.lastname: Péault
mesh_terms: D052436:Adipose Tissue, White / Q000166:cytology; D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D001808:Blood Vessels / Q000166:cytology; D002469:Cell Separation; D005434:Flow Cytometry; D006801:Humans; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D044968:Regenerative Medicine
chemical_list: D015703:Antigens, CD
keywords: mesenchymal stem cells; pericytes; flow cytometry; perivascular cells