title: Culturing hybridoma cell lines for monoclonal antibody production.
abstract: This protocol describes how to culture hybridoma cell lines (e.g., Thy1.1) for monoclonal antibody production. Supernatants harvested from such cultures can be used to purify various rodent neural cell types by immunopanning.
authors.affiliation: Department of Neurobiology, Stanford University School of Medicine, Stanford, California 94305, USA.
authors.forename: Alissa
authors.initials: A
authors.lastname: Winzeler
authors.forename: Jack T
authors.initials: JT
authors.lastname: Wang
mesh_terms: D000911:Antibodies, Monoclonal / Q000302:isolation & purification*; D018929:Cell Culture Techniques / Q000379:methods*; D002460:Cell Line; D003470:Culture Media / Q000737:chemistry; D006825:Hybridomas / Q000378:metabolism*
chemical_list: D000911:Antibodies, Monoclonal; D003470:Culture Media