title: Increase of the adhesion ability and display of a rumen fungal xylanase on the cell surface of Lactobacillus casei by using a listerial cell-wall-anchoring protein.
abstract: BACKGROUND
  Lactobacillus, which has great adhesion ability to intestinal mucosa and is able to hydrolyse plant cell walls, can be used more efficiently as a feed additive. To increase the adhesion ability and display a fungal xylanase on the cell surface of Lactobacillus casei, the Listeria monocytogenes cell-wall-anchoring protein gene, mub, was introduced into L. casei ATCC 393 cells and used as a fusion partner to display the rumen fungal xylanase XynCDBFV on the cell surface of the transformed strains.
  
  
  RESULTS
  The transformed strain L. casei pNZ-mub, which harboured mub gene, displayed recombinant Mub on its cell surface and showed greater adhesion ability to Caco-2 cells than the parental strain. The transformed strain L. casei pNZ-mub/xyn, which harboured mub-xynCDBFV fusion gene, acquired the capacity to break down oat spelt xylan and exhibited greater competition ability against the adhesion of L. monocytogenes to Caco-2 cells, in comparison with the parental strain.
  
  
  CONCLUSIONS
  Mub has a potential to be used as a fusion partner to display heterologous proteins on the cell surface of Lactobacillus. Moreover, this is the first report of the successful display of xylanase on the cell surface of Lactobacillus.
authors.affiliation: Department of Animal Science and Technology, National Taiwan University, Taipei, Taiwan.
authors.forename: Hsiang-Yun
authors.initials: HY
authors.lastname: Hsueh
authors.forename: Bi
authors.initials: B
authors.lastname: Yu
authors.forename: Chi-Te
authors.initials: CT
authors.lastname: Liu
authors.forename: Je-Ruei
authors.initials: JR
authors.lastname: Liu
mesh_terms: D000818:Animals; D018554:Avena / Q000378:metabolism; D001422:Bacterial Adhesion; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D018938:Caco-2 Cells; D002473:Cell Wall / Q000378:metabolism; D005503:Food Additives; D005656:Fungal Proteins / Q000378:metabolism; D005798:Genes, Bacterial; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism*; D007780:Lacticaseibacillus casei / Q000201:enzymology* / Q000235:genetics; D008089:Listeria monocytogenes / Q000201:enzymology* / Q000235:genetics; D019936:Probiotics; D011994:Recombinant Proteins / Q000378:metabolism; D012417:Rumen; D014170:Transformation, Genetic; D014990:Xylans / Q000378:metabolism; D014995:Xylosidases / Q000235:genetics / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D005503:Food Additives; D005656:Fungal Proteins; D011994:Recombinant Proteins; D014990:Xylans; D014995:Xylosidases
keywords: Lactobacillus casei; Listeria monocytogenes; cell-surface display; cell-wall-anchoring protein; xylanase