title: Association Between ABCB1 (MDR1) Gene Polymorphism and Unresponsiveness Combined Therapy in Chronic Hepatitis C virus.
abstract: BACKGROUND
  To treat viral infection of chronic hepatitis C (CHC) is a main strategy to prevent progression of liver disease, and cancer. Some patients with CHC have failed to respond to the common antiviral therapy in different populations.
  
  
  OBJECTIVE
  In the current study it was aimed to find out the possible role of multiple drug resistance gene1 (MDR1) in non-responder patients with CHC infection in Turkish population.
  
  
  METHODS
  Peripheral blood-EDTA samples were used for total genomic DNA isolation. In total of 55 patients with chronic hepatitis C and positive results for genotype 1 [31 male (56.4%), 24 female (43.6%) and mean age-min-max; 56.9 ± 9.66 (39-71)]; 19 responder (34.5%), 21 non responder (38.2%), and 15 recurrence (27.3%) were included in the presented results. Functional MDR1 gene was genotyped by multiplex PCR-based reverse-hybridization Strip Assay method, and some samples were confirmed by direct sequencing.
  
  
  RESULTS
  Our results indicate that MDR1 gene polymorphism is strongly associated with non-responder patients and those with recurrent chronic hepatitis C during conventional drug therapy when compared to the responder patients. Homozygous of the TT genotype for MDR1 exon 26 polymorphism was at 2.0-fold higher risk of non-responder than patients with CC and CT.
  
  
  CONCLUSIONS
  The homozygous MDR1 3435TT genotype which encodes the xenobiotic transporter P-glycoprotein may be associated with a poor antiviral response in HCV chronicity during conventional therapy, and large-scale studies are needed to validate this association.
authors.affiliation: Department of Gastroenterology, Faculty of Medicine, Cumhuriyet University, Sivas, Turkey.
authors.forename: Meryem
authors.initials: M
authors.lastname: Timucin
authors.forename: Hakan
authors.initials: H
authors.lastname: Alagozlu
authors.forename: Semra
authors.initials: S
authors.lastname: Ozdemir
authors.forename: Ozturk
authors.initials: O
authors.lastname: Ozdemir
keywords: Data Collection; Hepatitis C; P Glycoprotein