title: Identification of quantitative trait loci underlying milk traits in Spanish dairy sheep using linkage plus combined linkage disequilibrium and linkage analysis approaches.
abstract: In this study, 2 procedures were used to analyze a data set from a whole-genome scan, one based on linkage analysis information and the other combing linkage disequilibrium and linkage analysis (LDLA), to determine the quantitative trait loci (QTL) influencing milk production traits in sheep. A total of 1,696 animals from 16 half-sib families were genotyped using the OvineSNP50 BeadChip (Illumina Inc., San Diego, CA) and analysis was performed using a daughter design. Moreover, the same data set has been previously investigated through a genome-wide association (GWA) analysis and a comparison of results from the 3 methods has been possible. The linkage analysis and LDLA methodologies yielded different results, although some significantly associated regions were common to both procedures. The linkage analysis detected 3 overlapping genome-wise significant QTL on sheep chromosome (OAR) 2 influencing milk yield, protein yield, and fat yield, whereas 34 genome-wise significant QTL regions were detected using the LDLA approach. The most significant QTL for protein and fat percentages was detected on OAR3, which was reported in a previous GWA analysis. Both the linkage analysis and LDLA identified many other chromosome-wise significant associations across different sheep autosomes. Additional analyses were performed on OAR2 and OAR3 to determine the possible causality of the most significant polymorphisms identified for these genetic effects by the previously reported GWA analysis. For OAR3, the analyses demonstrated additional genetic proof of the causality previously suggested by our group for a single nucleotide polymorphism located in the α-lactalbumin gene (LALBA). In summary, although the results shown here suggest that in commercial dairy populations, the LDLA method exhibits a higher efficiency to map QTL than the simple linkage analysis or linkage disequilibrium methods, we believe that comparing the 3 analysis methods is the best approach to obtain a global picture of all identifiable QTL segregating in the population at both family-based and population-based levels.
authors.affiliation: Departamento de Producción Animal, Facultad de Veterinaria, Universidad de León, 24071 León, Spain.
authors.forename: E
authors.initials: E
authors.lastname: Garcia-Gámez
authors.forename: B
authors.initials: B
authors.lastname: Gutiérrez-Gil
authors.forename: A
authors.initials: A
authors.lastname: Suarez-Vega
authors.forename: L F
authors.initials: LF
authors.lastname: de la Fuente
authors.forename: J J
authors.initials: JJ
authors.lastname: Arranz
mesh_terms: D000818:Animals; D005260:Female; D008040:Genetic Linkage / Q000235:genetics*; D005819:Genetic Markers / Q000235:genetics; D007774:Lactation / Q000235:genetics*; D015810:Linkage Disequilibrium / Q000235:genetics*; D008892:Milk / Q000737:chemistry; D020411:Oligonucleotide Array Sequence Analysis / Q000662:veterinary; D010641:Phenotype; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D040641:Quantitative Trait Loci / Q000235:genetics*; D019655:Quantitative Trait, Heritable; D012756:Sheep / Q000235:genetics*
chemical_list: D005819:Genetic Markers
keywords: combined linkage disequilibrium and linkage analysis method; milk trait; quantitative trait loci; sheep