title: Potential chemoprotective effects of selenium on diazinon-induced DNA damage in rat peripheral blood lymphocyte.
abstract: The purpose of this study was to investigate the protective effects of selenium (Se) against genotoxicity induced by diazinon (DZN) in rat peripheral blood lymphocytes by micronucleus (MN) test. Animals were concurrently administered intraperitoneally with DZN in proper solvent (20 mg/kg body weight (b.w.)) and Se at three different doses (0.5, 1, and 2 mg/kg b.w.) for 30 consecutive days. The positive control group received DZN at the same dose without Se. After 24 h of last injection, 0.5 ml blood of each rat was received and cultured in culture medium for 44 h. The lymphocyte cultures were mitogenically stimulated with cytochalasin B to allow the evaluation of number of MNs in cytokinesis-blocked binucleated cells. Incubation of lymphocytes with DZN induced additional genotoxicity and is shown by increase in MNs frequency in human lymphocytes. Se at low dose of 0.5 mg/kg had a maximum effect and significantly reduced the MNs frequency in cultured lymphocytes (p < 0.0001) that reduced the frequency of MN from 12.78 ± 0.24% for DZN group to 4.40 ± 0.36. The present study revealed that Se particularly at low doses has a potent antigenotoxic effect against DZN -induced toxicity in rats, which may be due to the scavenging of free radicals and increased antioxidant status.
authors.affiliation: Pharmaceutical Sciences Research Center, Department of Toxicology and Pharmacology, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Islamic Republic of Iran.
authors.forename: M
authors.initials: M
authors.lastname: Shokrzadeh
authors.forename: N
authors.initials: N
authors.lastname: Ahangar
authors.forename: M
authors.initials: M
authors.lastname: Abdollahi
authors.forename: A
authors.initials: A
authors.lastname: Shadboorestan
authors.forename: M
authors.initials: M
authors.lastname: Omidi
authors.forename: S S Hosseini
authors.initials: SS
authors.lastname: Payam
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002478:Cells, Cultured; D004249:DNA Damage / Q000187:drug effects*; D003976:Diazinon; D007306:Insecticides; D008214:Lymphocytes / Q000187:drug effects / Q000378:metabolism; D008297:Male; D015162:Micronucleus Tests; D009153:Mutagens; D051381:Rats; D017208:Rats, Wistar; D012643:Selenium / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D007306:Insecticides; D009153:Mutagens; D012643:Selenium; D003976:Diazinon
keywords: Chemoprotective; diazinon; genotoxicity; micronucleus assay; rat blood lymphocyte; selenium