title: Impaired HDL function in obese adolescents: impact of lifestyle intervention and bariatric surgery.
abstract: OBJECTIVE
  HDL regulates endothelial function via stimulation of nitric oxide production. It is documented that endothelial function is impaired in obese adolescents, and improved by lifestyle interventions (LI).
  
  
  METHODS
  HDL function in obese adolescents and the impact of LI or Roux-en-Y gastric bypass surgery (RYGB) was assessed. HDL was isolated from 14 adolescents with normal body mass index (HDLcontrol ), 10 obese (HDLobese ) before and after 6 month LI, and five severe obese adolescents before and one year after RYGB. HDL-mediated phosphorylation of endothelial nitric oxide synthase (eNOS)-Ser(1177) , eNOS-Thr(495) , and PKC-ßII was evaluated. In addition the HDL proteome was analyzed.
  
  
  RESULTS
  HDLobese -mediated eNOS-Ser(1177) phosphorylation was reduced, whereas eNOS-Thr(495) phosphorylation increased significantly when compared to HDLcontrol . No impact of obesity was observed on PKC-ßII phosphorylation. LI and RYGB had no impact on HDL-mediated phosphorylation of eNOS and PKC-ßII. A principle component plot analysis of the HDL particle separated controls and severe obese, whereas the interventions did not trigger sufficient differences to the HDL proteome to permit distinction.
  
  
  CONCLUSIONS
  These results demonstrated that HDL-function is impaired in obese adolescents, and that LI or RYGB did not correct this dysfunction. This might be an argument for developing earlier prevention strategies in obese adolescents to avoid HDL dysfunction.
authors.affiliation: University Leipzig-Heart Center Leipzig, Department of Cardiology, Leipzig, Germany; Takasaki General Medical Center, Division of Cardiology, Takasaki, Japan; Gunma University School of Medicine, Department of Cardiology, Maebashi, Japan.
authors.forename: Yae
authors.initials: Y
authors.lastname: Matsuo
authors.forename: Andreas
authors.initials: A
authors.lastname: Oberbach
authors.forename: Holger
authors.initials: H
authors.lastname: Till
authors.forename: Thomas H
authors.initials: TH
authors.lastname: Inge
authors.forename: Martin
authors.initials: M
authors.lastname: Wabitsch
authors.forename: Anja
authors.initials: A
authors.lastname: Moss
authors.forename: Nico
authors.initials: N
authors.lastname: Jehmlich
authors.forename: Uwe
authors.initials: U
authors.lastname: Völker
authors.forename: Ulrike
authors.initials: U
authors.lastname: Müller
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Siegfried
authors.forename: Norio
authors.initials: N
authors.lastname: Kanesawa
authors.forename: Masahiko
authors.initials: M
authors.lastname: Kurabayashi
authors.forename: Gerhard
authors.initials: G
authors.lastname: Schuler
authors.forename: Axel
authors.initials: A
authors.lastname: Linke
authors.forename: Volker
authors.initials: V
authors.lastname: Adams
mesh_terms: D000293:Adolescent; D000808:Angiotensinogen / Q000378:metabolism; D016022:Case-Control Studies; D002648:Child; D008076:Cholesterol, HDL / Q000097:blood*; D042783:Endothelial Cells / Q000378:metabolism; D004727:Endothelium / Q000378:metabolism; D005260:Female; D005500:Follow-Up Studies; D015390:Gastric Bypass; D064368:Healthy Volunteers; D006801:Humans; D008019:Life Style; D008297:Male; D052250:Nitric Oxide Synthase Type III / Q000235:genetics / Q000378:metabolism; D063766:Pediatric Obesity / Q000097:blood / Q000601:surgery*; D010766:Phosphorylation; D064546:Protein Kinase C beta / Q000235:genetics / Q000378:metabolism; D020543:Proteome / Q000378:metabolism; D055815:Young Adult
chemical_list: D008076:Cholesterol, HDL; D020543:Proteome; D000808:Angiotensinogen; C496314:NOS3 protein, human; D052250:Nitric Oxide Synthase Type III; D064546:Protein Kinase C beta