title: The identification and characteristics of IL-22-producing T cells in acute graft-versus-host disease following allogeneic bone marrow transplantation.
abstract: Graft-versus-host disease (GVHD) remains the major obstacle for allogeneic bone marrow transplantation, in which many proinflammatory cytokines secreted by alloreactive donor T cells are involved. Role of IL-22 as a member of IL-10 family in GVHD is still disputed and the properties of IL-22-producing cells are unclear. We demonstrated here that CD4⁺ T cells but not CD8⁺ T cells involved in GVHD were the main cellular source of donor-derived IL-22. Th1 and Th17 cells were detected not only express classical cytokine IFN-γ or IL-17, but also contributed to IL-22 secretion in GVHD. Th22 cells characterized by the independent secretion of IL-22 were identified and occupied almost half percentage of IL-22-producing CD4⁺ T cells. The frequency of IL-22-producing CD4⁺ T cells showed dynamic changes with the development of GVHD. Finally, we observed that IL-22-producing CD4⁺ T cells in GVHD mouse carried CD62L⁻CD44(high/low) surface markers. In conclusion, we illuminate the characteristics of donor-derived IL-22-producing CD4⁺ T cells, which may have potent implication for further study of pathogenesis of GVHD.
authors.affiliation: Laboratory of Transplantation and Immunology, Xuzhou Medical College, No. 99 West Huaihai Road, Xuzhou 221002, Jiangsu, China; Department of Hematology, The Affiliated Hospital of Xuzhou Medical College, No. 99 West Huaihai Road, Xuzhou 221002, Jiangsu, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Zhao
authors.forename: Dongmei
authors.initials: D
authors.lastname: Zhao
authors.forename: Dong
authors.initials: D
authors.lastname: Huang
authors.forename: Xuguang
authors.initials: X
authors.lastname: Song
authors.forename: Chong
authors.initials: C
authors.lastname: Chen
authors.forename: Bin
authors.initials: B
authors.lastname: Pan
authors.forename: Qingyun
authors.initials: Q
authors.lastname: Wu
authors.forename: Jiang
authors.initials: J
authors.lastname: Cao
authors.forename: Yao
authors.initials: Y
authors.lastname: Yao
authors.forename: Lingyu
authors.initials: L
authors.lastname: Zeng
authors.forename: Kailin
authors.initials: K
authors.lastname: Xu
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D016026:Bone Marrow Transplantation; D002478:Cells, Cultured; D018450:Disease Progression; D006086:Graft vs Host Disease / Q000276:immunology*; D007371:Interferon-gamma / Q000378:metabolism; D020381:Interleukin-17 / Q000378:metabolism; D007378:Interleukins / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D016176:T-Lymphocyte Subsets / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*; D014184:Transplantation, Homologous; D000097043:Interleukin-22
chemical_list: D015703:Antigens, CD; D007371:Interferon-gamma; D020381:Interleukin-17; D007378:Interleukins; D000097043:Interleukin-22
keywords: BMT; BS; Bone marrow transplantation (BMT); GVHD; Graft-versus-host disease (GVHD); IL; IL-22; T cell; TBI; bone marrow plus splenic lymphocytes transplantation; bone marrow transplantation; graft-versus-host disease; interleukin; total body irradiation