title: Common versus noble Bacillus subtilis differentially responds to air and argon gas plasma.
abstract: The applications of low-temperature plasma are not only confined to decontamination and sterilization but are also found in the medical field in terms of wound and skin treatment. For the improvement of already established and also for new plasma techniques, in-depth knowledge on the interactions between plasma and microorganism is essential. In an initial study, the interaction between growing Bacillus subtilis and argon plasma was investigated by using a growth chamber system suitable for low-temperature gas plasma treatment of bacteria in liquid medium. In this follow-up investigation, a second kind of plasma treatment-namely air plasma-was applied. With combined proteomic and transcriptomic analyses, we were able to investigate the plasma-specific stress response of B. subtilis toward not only argon but also air plasma. Besides an overlap of cellular responses due to both argon and air plasma treatment (DNA damage and oxidative stress), a variety of gas-dependent cellular responses such as growth retardation and morphological changes were observed. Only argon plasma treatments lead to a phosphate starvation response whereas air plasma induced the tryptophan operon implying damage by photooxidation. Biological findings were supported by the detection of reactive plasma species by optical emission spectroscopy and Fourier transformed infrared spectroscopy measurements.
authors.affiliation: Institute for Microbiology, Ernst-Moritz-Arndt-University, Greifswald, Germany.
authors.forename: Theresa
authors.initials: T
authors.lastname: Winter
authors.forename: Jörg
authors.initials: J
authors.lastname: Bernhardt
authors.forename: Jörn
authors.initials: J
authors.lastname: Winter
authors.forename: Ulrike
authors.initials: U
authors.lastname: Mäder
authors.forename: Rabea
authors.initials: R
authors.lastname: Schlüter
authors.forename: Klaus-Dieter
authors.initials: KD
authors.lastname: Weltmann
authors.forename: Michael
authors.initials: M
authors.lastname: Hecker
authors.forename: Harald
authors.initials: H
authors.lastname: Kusch
mesh_terms: D000388:Air; D001128:Argon / Q000737:chemistry / Q000494:pharmacology*; D001412:Bacillus subtilis / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D004249:DNA Damage; D020869:Gene Expression Profiling; D050296:Microbial Viability; D018384:Oxidative Stress; D056951:Photoelectron Spectroscopy; D058626:Plasma Gases / Q000737:chemistry / Q000494:pharmacology*; D040901:Proteomics; D017382:Reactive Oxygen Species / Q000378:metabolism; D017550:Spectroscopy, Fourier Transform Infrared; D013312:Stress, Physiological
chemical_list: D058626:Plasma Gases; D017382:Reactive Oxygen Species; D001128:Argon
keywords: Bacillus subtilis; Bielectric barrier discharge; Microbiology; Plasma-microorganism interaction; Transcriptomics