title: Gombamide A, a cyclic thiopeptide from the sponge Clathria gombawuiensis.
abstract: A new peptide, gombamide A (1), was isolated from the marine sponge Clathria gombawuiensis, collected from Korean waters. On the basis of the results of combined spectroscopic analyses, the structure of this compound was determined to be a cyclic C-terminally modified thiohexapeptide containing the unusual amino acid residues para-hydroxystyrylamide (pHSA) and pyroglutamic acid (pyroGlu). The absolute configurations of all amino acid residues were determined to be l by advanced Marfey's analysis. The new compound exhibited weak cytotoxicity against A549 and K562 cell lines as well as moderate inhibitory activity against Na(+)/K(+)-ATPase.
authors.affiliation: Natural Products Research Institute, College of Pharmacy, Seoul National University, San 56-1, Sillim, Gwanak, Seoul 151-742, Korea.
authors.forename: Jung-Kyun
authors.initials: JK
authors.lastname: Woo
authors.forename: Ju-eun
authors.initials: JE
authors.lastname: Jeon
authors.forename: Chang-Kwon
authors.initials: CK
authors.lastname: Kim
authors.forename: Chung J
authors.initials: CJ
authors.lastname: Sim
authors.forename: Dong-Chan
authors.initials: DC
authors.lastname: Oh
authors.forename: Ki-Bong
authors.initials: KB
authors.lastname: Oh
authors.forename: Jongheon
authors.initials: J
authors.lastname: Shin
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D008386:Marine Biology; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D010456:Peptides, Cyclic / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D011161:Porifera / Q000737:chemistry*; D056910:Republic of Korea; D000254:Sodium-Potassium-Exchanging ATPase / Q000037:antagonists & inhibitors
chemical_list: D000970:Antineoplastic Agents; D010456:Peptides, Cyclic; C584229:gombamide A; D000254:Sodium-Potassium-Exchanging ATPase