title: Proteomic and metabolomic profiles of marine Vibrio sp. 010 in response to an antifoulant challenge.
abstract: Vibrio spp. have the ability to form biofilms, which may contribute to the subsequent successful colonization by microfouling and macrofouling organisms. The effects of an antifouling compound, poly-ether B, on Vibrio sp. 010 were investigated using flow cytometry, proteomics, and metabolomics. A 2-D gel-based proteomic analysis was used to identify proteins responsive to poly-ether B treatment. The profiles of biofilm metabolites were analyzed by ultra-performance liquid chromatography-mass spectrometry. Poly-ether B caused a significant reduction in viability. The proteins affected by the treatment were related to nucleotide metabolism, the glyoxylate cycle, and stress responses. Metabolites such as tripeptides, fatty acids, and quorum-sensing molecules were regulated differentially. Down-regulation of proteins and metabolites potentially led to a loss in colonisation ability, thereby affecting the structure of the biofilm. These results suggest that the proteins and metabolites identified may serve as target molecules for potent antifouling compounds.
authors.affiliation: KAUST Global Collaborative Research, Division of Life Science, The Hong Kong University of Science and Technology, Hong Kong SAR, China.
authors.forename: Kondethimmanahalli H
authors.initials: KH
authors.lastname: Chandramouli
authors.forename: Swagatika
authors.initials: S
authors.lastname: Dash
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhang
authors.forename: Timothy
authors.initials: T
authors.lastname: Ravasi
authors.forename: Pei-Yuan
authors.initials: PY
authors.lastname: Qian
mesh_terms: D018441:Biofilms / Q000187:drug effects; D057219:Biofouling / Q000517:prevention & control*; D002853:Chromatography, Liquid; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects; D013058:Mass Spectrometry; D055432:Metabolomics; D011092:Polyethylene Glycols / Q000494:pharmacology*; D011409:Propylene Glycols / Q000494:pharmacology*; D040901:Proteomics; D014733:Vibrio / Q000187:drug effects / Q000378:metabolism* / Q000502:physiology
chemical_list: D011409:Propylene Glycols; D011092:Polyethylene Glycols