title: Bioinspired polydopamine as the scaffold for the active AuNPs anchoring and the chemical simultaneously reduced graphene oxide: characterization and the enhanced biosensing application.
abstract: We report here an efficient approach to enhance the performance of biosensing platform based on graphene or graphene derivate. Initially, graphene oxides (GO) nanosheets were reduced and surface functionalized by one-step oxidative polymerization of dopamine in basic solution at environment friendly condition to obtain the polydopamine (Pdop) modified reduced graphene oxides (PDRGO). The bioinspired surface was further used as a support to anchor active gold nanoparticles (AuNPs). The morphology and structure of the as-prepared AuNPs/PDRGO nanocomposite were investigated by field-emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM), Fourier transform-infrared spectroscopy (FT-IR). Electrochemical studies demonstrate that the as-prepared AuNPs/PDRGO hybrid materials possess excellent electrochemical properties and electrocatalytic activity toward the oxidation of NADH at low potential (0.1 V vs. SCE) with the fast response (15s) and the broad linear range (5.0 × 10(-8)-4.2 × 10(-5)M). Thus, this AuNPs/PDRGO nanocomposite can be further used to fabricate a sensitive alcohol biosensor using alcohol dehydrogenase (ADH), by simply incorporating the specific enzyme within the composite matrix with the aid of chitosan (Chit).
authors.affiliation: School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing 210094, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Tian
authors.forename: Sheng-Yuan
authors.initials: SY
authors.lastname: Deng
authors.forename: Da-Li
authors.initials: DL
authors.lastname: Li
authors.forename: Dan
authors.initials: D
authors.lastname: Shan
authors.forename: Wei
authors.initials: W
authors.lastname: He
authors.forename: Xue-Ji
authors.initials: XJ
authors.lastname: Zhang
authors.forename: You
authors.initials: Y
authors.lastname: Shi
mesh_terms: D000426:Alcohol Dehydrogenase / Q000378:metabolism; D015374:Biosensing Techniques / Q000379:methods*; D004800:Enzymes, Immobilized / Q000378:metabolism; D000431:Ethanol / Q000032:analysis* / Q000378:metabolism; D006046:Gold / Q000737:chemistry*; D006108:Graphite / Q000737:chemistry*; D007211:Indoles / Q000737:chemistry*; D057230:Limit of Detection; D009243:NAD / Q000032:analysis*; D053758:Nanoparticles / Q000737:chemistry*; D010084:Oxidation-Reduction; D010087:Oxides / Q000737:chemistry; D011108:Polymers / Q000737:chemistry*; D012441:Saccharomyces cerevisiae / Q000201:enzymology
chemical_list: D004800:Enzymes, Immobilized; D007211:Indoles; D010087:Oxides; D011108:Polymers; C568283:polydopamine; D009243:NAD; D000431:Ethanol; D006046:Gold; D006108:Graphite; D000426:Alcohol Dehydrogenase
keywords: Alcohol dehydrogenase; AuNPs; Graphene oxide; NADH; Polydopamine; Surface functionalization