title: Antimicrobial activity of the synthetic peptide scolopendrasin ii from the centipede Scolopendra subspinipes mutilans.
abstract: The centipede Scolopendra subpinipes mutilans is a medicinally important arthropod species. However, its transcriptome is not currently available and transcriptome analysis would be useful in providing insight into a molecular level approach. Hence, we performed de novo RNA sequencing of S. subpinipes mutilans using next-generation sequencing. We generated a novel peptide (scolopendrasin II) based on a SVM algorithm, and biochemically evaluated the in vitro antimicrobial activity of scolopendrasin II against various microbes. Scolopendrasin II showed antibacterial activities against gram-positive and -negative bacterial strains, including the yeast Candida albicans and antibiotic-resistant gram-negative bacteria, as determined by a radial diffusion assay and colony count assay without hemolytic activity. In addition, we confirmed that scolopendrasin II bound to the surface of bacteria through a specific interaction with lipoteichoic acid and a lipopolysaccharide, which was one of the bacterial cell-wall components. In conclusion, our results suggest that scolopendrasin II may be useful for developing peptide antibiotics.
authors.affiliation: Department of Agricultural Biology, National Academy of Agricultural Science, RDA, Suwon 441-853, Republic of Korea.
authors.forename: Young-Nam
authors.initials: YN
authors.lastname: Kwon
authors.forename: Joon Ha
authors.initials: JH
authors.lastname: Lee
authors.forename: In-Woo
authors.initials: IW
authors.lastname: Kim
authors.forename: Sang-Hee
authors.initials: SH
authors.lastname: Kim
authors.forename: Eun-Young
authors.initials: EY
authors.lastname: Yun
authors.forename: Sung-Hee
authors.initials: SH
authors.lastname: Nam
authors.forename: Mi-Young
authors.initials: MY
authors.lastname: Ahn
authors.forename: Mihye
authors.initials: M
authors.lastname: Jeong
authors.forename: Dong-Chul
authors.initials: DC
authors.lastname: Kang
authors.forename: In Hee
authors.initials: IH
authors.lastname: Lee
authors.forename: Jae Sam
authors.initials: JS
authors.lastname: Hwang
mesh_terms: D000818:Animals; D023181:Antimicrobial Cationic Peptides / Q000235:genetics / Q000302:isolation & purification / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects*; D002176:Candida albicans / Q000187:drug effects*; D002473:Cell Wall / Q000378:metabolism; D000077743:Diterpene Alkaloids; D004365:Drugs, Chinese Herbal; D008826:Microbial Sensitivity Tests; D011485:Protein Binding; D011994:Recombinant Proteins / Q000235:genetics / Q000302:isolation & purification / Q000494:pharmacology
chemical_list: D023181:Antimicrobial Cationic Peptides; D000077743:Diterpene Alkaloids; D004365:Drugs, Chinese Herbal; D011994:Recombinant Proteins; C574917:scolopendra alkaloids