title: Lipid mediator serum profiles in asthmatics significantly shift following dietary supplementation with omega-3 fatty acids.
abstract: METHODS
  In contrast to well-characterized PUFA levels in serum, little is known regarding their downstream metabolic products. However, many of these compounds are lipid mediators with prominent roles during pro- and antiinflammatory processes.
  
  
  RESULTS
  In this double blind crossover study on asthmatics, shifts in serum levels of ω-3 and ω-6 PUFA-derived oxidized fatty acids (e.g. eicosanoids, oxylipins) were quantified following dietary fish oil supplementation. Serum was obtained from subjects following fasting at three occasions; (i) prior to supplementation, (ii) following a 3-week supplement intake of either placebo or fish oil, and (iii) following a 3-week washout period with a subsequent 3-week period of either fish oil or placebo supplement. A total of 87 oxylipins representing cyclooxygenase (COX), lipoxygenase (LOX), and cytochrome P450 (CYP) metabolic pathways were screened via LC-MS/MS. The primary alterations observed were in CYP- and 15-LOX-derived EPA- and CYP-derived DHA oxylipins.
  
  
  CONCLUSIONS
  The results indicate that intake of an ω-3 rich diet alters not only the PUFA ratio, but also the ratio of downstream oxylipins. These data further support that dietary manipulation with ω-3 PUFAs affects not only PUFA levels, but importantly also the downstream metabolic profile.
authors.affiliation: Department of Medical Biochemistry and Biophysics, Division of Physiological Chemistry II, Karolinska Institutet, Stockholm, Sweden.
authors.forename: Susanna L
authors.initials: SL
authors.lastname: Lundström
authors.forename: Jun
authors.initials: J
authors.lastname: Yang
authors.forename: John D
authors.initials: JD
authors.lastname: Brannan
authors.forename: Jesper Z
authors.initials: JZ
authors.lastname: Haeggström
authors.forename: Bruce D
authors.initials: BD
authors.lastname: Hammock
authors.forename: Parameswaran
authors.initials: P
authors.lastname: Nair
authors.forename: Paul
authors.initials: P
authors.lastname: O'Byrne
authors.forename: Sven-Erik
authors.initials: SE
authors.lastname: Dahlén
authors.forename: Craig E
authors.initials: CE
authors.lastname: Wheelock
mesh_terms: D000328:Adult; D001249:Asthma / Q000178:diet therapy*; D019587:Dietary Supplements; D004281:Docosahexaenoic Acids / Q000097:blood; D015118:Eicosapentaenoic Acid / Q000097:blood; D015525:Fatty Acids, Omega-3 / Q000097:blood / Q000627:therapeutic use*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D015999:Multivariate Analysis; D054883:Oxylipins / Q000097:blood*; D010743:Phospholipids / Q000097:blood / Q000737:chemistry; D055815:Young Adult
chemical_list: D015525:Fatty Acids, Omega-3; D054883:Oxylipins; D010743:Phospholipids; D004281:Docosahexaenoic Acids; D015118:Eicosapentaenoic Acid
keywords: Asthma; Eicosanoid; Lipidomics; Omega-3; Oxylipin