title: Efficacy of intra-tendinous injection of platelet-rich plasma in treating tendinosis: comprehensive assessment of a rat model.
abstract: OBJECTIVE
  To assess the potential of intra-tendinous injection of platelet rich plasma (PRP) to treat tendinosis (T+) in a rat model of patellar and Achilles T+, and evaluate its local toxicity.
  
  
  METHODS
  Thirty rats (120 patellar and Achilles tendons) were used. We induced T+ into 80 tendons (patellar = 40, Achilles = 40) by injecting collagenase at day 0 under ultrasound (US) guidance. Clinical examination and US at day 3, followed by US-guided intra-tendinous injection of either PRP (PRPT+, n = 40) or physiological serum (ST+, n = 40, control). Follow-up was at days 6, 13, 18 and 25 using clinical, US and histological evaluation. To study PRP toxicity, we injected PRP into 40 normal tendons (PRPT-) and compared with 40 untreated normal tendons (T-).
  
  
  RESULTS
  All PRPT+ showed better joint mobilisation compared with ST+ at day 6 (P = 0.005), day 13 (P = 0.02), day 18 (P = 0.003) and day 25 (P = 0.01). Similar results were found regarding US and histology, with smaller collagen fibre diameters (day 6, P = 0.003, day 25, P ≤ 0.004), less disorganisation and fewer neovessels (day 6, P = 0.003, day 25, P = 0.0003) in PRPT+ compared with ST+. Comparison between PRPT- and T- showed no PRP toxicity (P = 0.18).
  
  
  CONCLUSIONS
  Our study suggests that mono-injection of PRP in T+ improves tendon healing, with no local toxicity.
  
  
  CONCLUSIONS
  • We assessed the potential of platelet rich plasma (PRP) to treat tendinosis. • We treated patellar and Achilles tendinosis in a rat model. • We evaluated clinical, imaging and histological data. • Intra-tendinous PRP injection could be useful in the treatment of tendinosis.
authors.affiliation: Service de Radiologie, Hôpital universitaire Bichat, Paris, France, benjamin.dallaudiere@gmail.com.
authors.forename: Benjamin
authors.initials: B
authors.lastname: Dallaudière
authors.forename: Marta
authors.initials: M
authors.lastname: Lempicki
authors.forename: Lionel
authors.initials: L
authors.lastname: Pesquer
authors.forename: Liliane
authors.initials: L
authors.lastname: Louedec
authors.forename: Pierre Marie
authors.initials: PM
authors.lastname: Preux
authors.forename: Philippe
authors.initials: P
authors.lastname: Meyer
authors.forename: Vincent
authors.initials: V
authors.lastname: Hummel
authors.forename: Ahmed
authors.initials: A
authors.lastname: Larbi
authors.forename: Lydia
authors.initials: L
authors.lastname: Deschamps
authors.forename: Clement
authors.initials: C
authors.lastname: Journe
authors.forename: Agathe
authors.initials: A
authors.lastname: Hess
authors.forename: Alain
authors.initials: A
authors.lastname: Silvestre
authors.forename: Paul
authors.initials: P
authors.lastname: Sargos
authors.forename: Philippe
authors.initials: P
authors.lastname: Loriaut
authors.forename: Patrick
authors.initials: P
authors.lastname: Boyer
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Schouman-Claeys
authors.forename: Jean Baptiste
authors.initials: JB
authors.lastname: Michel
authors.forename: Jean Michel
authors.initials: JM
authors.lastname: Serfaty
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D006801:Humans; D015552:Injections, Intralesional; D008297:Male; D017713:Platelet Transfusion / Q000379:methods*; D053657:Platelet-Rich Plasma / Q000000981:diagnostic imaging*; D051381:Rats; D017207:Rats, Sprague-Dawley; D052256:Tendinopathy / Q000000981:diagnostic imaging* / Q000628:therapy*; D016896:Treatment Outcome; D014463:Ultrasonography