title: Synergistic effect of black tea and curcumin in improving the hepatotoxicity induced by aflatoxin B1 in rats.
abstract: Aflatoxin B1 (AFB1) is a toxic compound commonly found as a contaminant in human food. It is carcinogenic due its potential in inducing the oxidative stress and distortion of the most antioxidant enzymes. Since black tea possesses strong antioxidant activity, it protects cells and tissues against oxidative stress. Curcumin (CMN), a naturally occurring agent, has a combination of biological and pharmacological properties that include antioxidant activity. Therefore, the present study was carried out to investigate the possible role of separate and mixed supplementation of black tea extract and CMN in the hepatotoxicity induced by AFB1 in rats. A total of 48: adult male Sprague Dawley rats were randomly divided into eight groups with six rats in each group. Group 1 (normal control) includes rats that received no treatment. Groups 2, 3, and 4 (positive control) include rats that received olive oil, black tea extract, and CMN, respectively. Group 5 includes rats that received AFB1 at a dose of 750 μg/kg body weight (b.w.) dissolved in olive oil. Groups 6, 7, and 8 include rats that received AFB1 along with 2% black tea extract, CMN at a dose of 200 mg/kg b.w., and both black tea extract and CMN at the same previous doses, respectively. After 90 days, biochemical and histopathological examination was carried out for the blood samples and liver tissues. A significant decrease in the antioxidant enzymes and a significant increase in the lipid peroxidation and hydrogen peroxide in the rats treated with AFB1 were observed. Moreover, there were dramatic changes in the liver function biomarkers, lipid profile, and liver architecture. Supplementation of black tea extract or CMN showed an efficient role in repairing the distortion of the biochemical and histological changes induced by AFB1 in liver. This improvement was more pronounced when both CMN and black tea were used together.
authors.affiliation: Department of Zoology, Faculty of Science, Tanta University, Tanta, Egypt abeer75875@hotmail.com.
authors.forename: Abeer A
authors.initials: AA
authors.lastname: Alm-Eldeen
authors.affiliation: Department of Zoology, Faculty of Science, Tanta University, Tanta, Egypt.
authors.forename: Mohamed H
authors.initials: MH
authors.lastname: Mona
authors.affiliation: Department of Biology, Faculty of Science, King Khalid University, Abha, Saudi Arabia.
authors.forename: Ali A
authors.initials: AA
authors.lastname: Shati
authors.affiliation: Department of Biology, Faculty of Science, King Khalid University, Abha, Saudi Arabia.
authors.forename: Haitham I
authors.initials: HI
authors.lastname: El-Mekkawy
mesh_terms: D016604:Aflatoxin B1 / Q000037:antagonists & inhibitors* / Q000633:toxicity; D000818:Animals; D000975:Antioxidants / Q000627:therapeutic use; D015415:Biomarkers / Q000097:blood / Q000378:metabolism; D028241:Camellia sinensis / Q000737:chemistry; D003474:Curcumin / Q000737:chemistry / Q000627:therapeutic use*; D019587:Dietary Supplements; D005511:Food Handling; D048550:Hepatic Insufficiency / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D050356:Lipid Metabolism / Q000187:drug effects; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000187:drug effects / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D008297:Male; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000187:drug effects; D010936:Plant Extracts / Q000819:agonists / Q000627:therapeutic use*; D018515:Plant Leaves / Q000737:chemistry; D011897:Random Allocation; D017207:Rats, Sprague-Dawley; D013662:Tea / Q000737:chemistry
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D010936:Plant Extracts; D013662:Tea; D016604:Aflatoxin B1; D003474:Curcumin
keywords: Aflatoxin B1; black tea; curcumin; histopathology; liver; oxidative stress; rat