title: A comparative in vitro evaluation of self-assembled PTX-PLA and PTX-MPEG-PLA nanoparticles.
abstract: We present a dialysis technique to direct the self-assembly of paclitaxel (PTX)-loaded nanoparticles (NPs) using methoxypolyethylene glycol-poly(d,l-lactide) (MPEG-PLA) and PLA, respectively. The composition, morphology, particle size and zeta potential, drug loading content, and drug encapsulation efficiency of both PTX-PLA NPs and PTX-MPEG-PLA NPs were characterized by X-ray diffraction, Fourier transform infrared spectroscopy, transmission electron microscopy, dynamic light scattering, electrophoretic light scattering, and high-performance liquid chromatography. The passive targeting effect and in vitro cell viability of the PTX-MPEG-PLA NPs on HeLa cells were demonstrated by comparative cellular uptake and MTT assay of the PTX-PLA NPs. The results showed that the PTX-MPEG-PLA NPs and PTX-PLA NPs presented a hydrodynamic particle size of 179.5 and 441.9 nm, with a polydispersity index of 0.172 and 0.189, a zeta potential of -24.3 and -42.0 mV, drug encapsulation efficiency of 18.3% and 20.0%, and drug-loaded content of 1.83% and 2.00%, respectively. The PTX-MPEG-PLA NPs presented faster release rate with minor initial burst compared to the PTX-PLA NPs. The PTX-MPEG-PLA NPs presented superior cell cytotoxicity and excellent cellular uptake compared to the PTX-PLA NPs. These results suggested that the PTX-MPEG-PLA NPs presented more desirable characteristics for sustained drug delivery compared to PTX-PLA NPs.
authors.affiliation: Department of Biomaterials, Research Institute for Biomimetics and Soft Matter, College of Materials, Xiamen University, Xiamen 361005, China. houzhenqing@xmu.edu.cn.
authors.forename: Fei
authors.initials: F
authors.lastname: Cui
authors.forename: Yang
authors.initials: Y
authors.lastname: Li
authors.forename: Shuifan
authors.initials: S
authors.lastname: Zhou
authors.forename: Mengmeng
authors.initials: M
authors.lastname: Jia
authors.forename: Xiangrui
authors.initials: X
authors.lastname: Yang
authors.forename: Fei
authors.initials: F
authors.lastname: Yu
authors.forename: Shefang
authors.initials: S
authors.lastname: Ye
authors.forename: Zhenqing
authors.initials: Z
authors.lastname: Hou
authors.forename: Liya
authors.initials: L
authors.lastname: Xie