title: Development of a real-time SYBR Green PCR assay for the rapid detection of Dermatophilus congolensis.
abstract: Methods such as real time (RT)-PCR have not been developed for the rapid detection and diagnosis of Dermatophilus (D.) congolensis infection. In the present study, a D. congolensis-specific SYBR Green RT-PCR assay was evaluated. The detection limit of the RT-PCR assay was 1 pg of DNA per PCR reaction. No cross-reaction with nucleic acids extracted from Pseudomonas aeruginosa, Mycobacterium tuberculosis, Staphylococcus aureus, or Austwickia chelonae was observed. Finally, the RT-PCR assay was used to evaluate clinical samples collected from naturally infected animals with D. congolensis. The results showed that this assay is a fast and reliable method for diagnosing dermatophilosis.
authors.affiliation: Departamento de Medicina y Zootecnia de Aves, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México, Ciudad de México 04510, México.
authors.forename: Alfredo
authors.initials: A
authors.lastname: García
authors.forename: Remigio
authors.initials: R
authors.lastname: Martínez
authors.forename: José Manuel
authors.initials: JM
authors.lastname: Benitez-Medina
authors.forename: David
authors.initials: D
authors.lastname: Risco
authors.forename: Waldo Luis
authors.initials: WL
authors.lastname: Garcia
authors.forename: Joaquín
authors.initials: J
authors.lastname: Rey
authors.forename: Juan Manuel
authors.initials: JM
authors.lastname: Alonso
authors.forename: Javier
authors.initials: J
authors.lastname: Hermoso de Mendoza
mesh_terms: D000192:Actinomycetales / Q000302:isolation & purification*; D000193:Actinomycetales Infections / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary*; D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis* / Q000382:microbiology; D005456:Fluorescent Dyes; D006734:Horse Diseases / Q000175:diagnosis* / Q000382:microbiology; D006736:Horses; D057230:Limit of Detection; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods* / Q000662:veterinary; D015203:Reproducibility of Results; D012756:Sheep; D012757:Sheep Diseases / Q000175:diagnosis* / Q000382:microbiology
chemical_list: D005456:Fluorescent Dyes
keywords: Dermatophilus congolensis; clinical samples; dermatophilosis; diagnosis; real-time PCR