title: The signal within the noise: efficient inference of stochastic gene regulation models using fluorescence histograms and stochastic simulations.
abstract: BACKGROUND
  In the noisy cellular environment, stochastic fluctuations at the molecular level manifest as cell-cell variability at the population level that is quantifiable using high-throughput single-cell measurements. Such variability is rich with information about the cell's underlying gene regulatory networks, their architecture and the parameters of the biochemical reactions at their core.
  
  
  RESULTS
  We report a novel method, called Inference for Networks of Stochastic Interactions among Genes using High-Throughput data (INSIGHT), for systematically combining high-throughput time-course flow cytometry measurements with computer-generated stochastic simulations of candidate gene network models to infer the network's stochastic model and all its parameters. By exploiting the mathematical relationships between experimental and simulated population histograms, INSIGHT achieves scalability, efficiency and accuracy while entirely avoiding approximate stochastic methods. We demonstrate our method on a synthetic gene network in bacteria and show that a detailed mechanistic model of this network can be estimated with high accuracy and high efficiency. Our method is completely general and can be used to infer models of signal-activated gene networks in any organism based solely on flow cytometry data and stochastic simulations.
  
  
  BACKGROUND
  A free C source code implementing the INSIGHT algorithm, together with test data is available from the authors.
  
  
  BACKGROUND
  mustafa.khammash@bsse.ethz.ch
  
  
  BACKGROUND
  Supplementary data are available at Bioinformatics online.
authors.affiliation: ETH Zürich, Department of Biosystems Science and Engineering, Mattenstrasse 26, 4058, Basel, Switzerland.
authors.forename: Gabriele
authors.initials: G
authors.lastname: Lillacci
authors.forename: Mustafa
authors.initials: M
authors.lastname: Khammash
mesh_terms: D000465:Algorithms; D001499:Bayes Theorem; D003198:Computer Simulation; D005434:Flow Cytometry; D005453:Fluorescence; D005786:Gene Expression Regulation; D053263:Gene Regulatory Networks; D008957:Models, Genetic; D013269:Stochastic Processes