title: Increased levels of circulating microparticles are associated with increased procoagulant activity in patients with cutaneous malignant melanoma.
abstract: Microparticles (MPs) are known to be increased in various malignancies and are involved in tumor invasion, angiogenesis, coagulation, and metastasis. We investigated the plasma levels of annexin-V MPs (AV(+)MPs), platelet-derived MPs (PMPs), and endothelial-derived MPs (EMPs) in patients with melanoma (n=129) and in healthy controls (n=49). A functional coagulation test STA Procoag-PPL measuring the clotting time was performed on samples containing MPs to evaluate their procoagulant potential. The plasma levels of PMPs, EMPs, and AV(+)MPs were significantly higher, and the clotting time-PPL was significantly lower in melanoma patients than in healthy controls. The plasma levels of PMPs, EMPs, and AV(+)MPs were higher in stage IV than in the other stages of melanoma, but with no significant difference. In addition, we observed an inverse correlation between PMPs, AV(+)MPs, and clotting times. Our data suggest that MPs are involved in the progression of melanoma and may be associated to melanoma-associated thrombogenesis.
authors.affiliation: Service de Dermatologie, Centre Hospitalier Universitaire Jean Minjoz, Besançon, France.
authors.forename: Claire
authors.initials: C
authors.lastname: Laresche
authors.affiliation: Service de Dermatologie, Centre Hospitalier Universitaire Jean Minjoz, Besançon, France; Université de Franche Comté, INSERM UMR 1098, SFR FED4234, Besançon, France. Electronic address: fabien.pelletier@univ-fcomte.fr.
authors.forename: Fabien
authors.initials: F
authors.lastname: Pelletier
authors.affiliation: Université de Franche Comté, INSERM UMR 1098, SFR FED4234, Besançon, France; Etablissement Français du Sang Bourgogne Franche Comté, Besançon, France.
authors.forename: Francine
authors.initials: F
authors.lastname: Garnache-Ottou
authors.affiliation: Service de Dermatologie, Centre Hospitalier Universitaire Jean Minjoz, Besançon, France.
authors.forename: Thomas
authors.initials: T
authors.lastname: Lihoreau
authors.affiliation: Université de Franche Comté, INSERM UMR 1098, SFR FED4234, Besançon, France; Etablissement Français du Sang Bourgogne Franche Comté, Besançon, France.
authors.forename: Sabeha
authors.initials: S
authors.lastname: Biichlé
authors.affiliation: Université de Franche Comté, INSERM UMR 1098, SFR FED4234, Besançon, France; Etablissement Français du Sang Bourgogne Franche Comté, Besançon, France.
authors.forename: Guillaume
authors.initials: G
authors.lastname: Mourey
authors.affiliation: Université de Franche Comté, INSERM UMR 1098, SFR FED4234, Besançon, France; Etablissement Français du Sang Bourgogne Franche Comté, Besançon, France.
authors.forename: Philippe
authors.initials: P
authors.lastname: Saas
authors.affiliation: Service de Dermatologie, Centre Hospitalier Universitaire Jean Minjoz, Besançon, France; Université de Franche Comté, INSERM UMR 1098, SFR FED4234, Besançon, France.
authors.forename: Philippe
authors.initials: P
authors.lastname: Humbert
authors.affiliation: Université de Franche Comté, INSERM UMR 1098, SFR FED4234, Besançon, France; Etablissement Français du Sang Bourgogne Franche Comté, Besançon, France.
authors.forename: Estelle
authors.initials: E
authors.lastname: Seilles
authors.affiliation: Service de Dermatologie, Centre Hospitalier Universitaire Jean Minjoz, Besançon, France; Université de Franche Comté, EA3181, SFR FED4234, Besançon, France.
authors.forename: François
authors.initials: F
authors.lastname: Aubin
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001777:Blood Coagulation / Q000502:physiology*; D001779:Blood Coagulation Factors / Q000378:metabolism*; D055252:Cell-Derived Microparticles / Q000378:metabolism*; D042783:Endothelial Cells / Q000378:metabolism; D005260:Female; D006801:Humans; D008297:Male; D008545:Melanoma / Q000378:metabolism* / Q000473:pathology; D008875:Middle Aged; D009367:Neoplasm Staging; D012878:Skin Neoplasms / Q000378:metabolism* / Q000473:pathology; D013927:Thrombosis / Q000378:metabolism*; D000096142:Cutaneous Malignant Melanoma
chemical_list: D001779:Blood Coagulation Factors; C045071:leukocyte procoagulant activity