title: Production of 10(S)-hydroxy-8(E)-octadecenoic and 7,10(S,S)-hydroxy-8(E)-octadecenoic ethyl esters by Novozym 435 in solvent-free media.
abstract: Novozym 435, lipase B from Candida antarctica, was used in this study for the production of ethyl esters. For the first time, trans-hydroxy-fatty acid ethyl esters were synthesized in vitro in solvent-free media. We studied the effects of the substrate-ethanol molar ratio and enzyme synthetic stability of the biocatalyst. To determine the structure of the formed compounds, Fourier transformed infrared spectroscopy, nuclear magnetic resonance, and matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry were used, three less time-consuming structural techniques. trans-Hydroxy-fatty acid ethyl esters were synthesized with a reaction yield of 90 % or higher with optimal reaction conditions.
authors.affiliation: Departament de Microbiologia i Parasitologia Sanitàries, Facultat de Farmàcia, Universitat de Barcelona, Av. Joan XXIII s/n, 08028, Barcelona, Spain.
authors.forename: Ignacio
authors.initials: I
authors.lastname: Martin-Arjol
authors.forename: Montse
authors.initials: M
authors.lastname: Busquets
authors.forename: Terry A
authors.initials: TA
authors.lastname: Isbell
authors.forename: Angels
authors.initials: A
authors.lastname: Manresa
mesh_terms: D055162:Biocatalysis; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry; D004952:Esters / Q000737:chemistry*; D005656:Fungal Proteins / Q000737:chemistry*; D008049:Lipase / Q000737:chemistry*; D015394:Molecular Structure; D013229:Stearic Acids / Q000737:chemistry*
chemical_list: C114874:5-octadecenoic acid; D004800:Enzymes, Immobilized; D004952:Esters; D005656:Fungal Proteins; D013229:Stearic Acids; C093667:Novozyme 435; D008049:Lipase