title: [Expression of Dickkopf-1 in human colon carcinoma cell lines].
abstract: OBJECTIVE
  To investigate the expression of Dickkopf-1(DKK1) in 6 different colon carcinoma cell lines.
  
  
  METHODS
  The expression of DKK1 protein were detected in SW480, SW620, HT29, LS174T, LOVO, and HCT116 cells lines using Western blot analysis and immunocytochemistry, and the mRNA levels of DKK1 was detected using quantitative real-time PCR (qPCR).
  
  
  RESULTS
  The results of qPCR showed significantly higher expressions of DKK1 mRNA in HT29 and HCT116 cells than in the other 4 cells (P<0.05). Western blotting demonstrated that DKK1 was over-expressed in HCT116 and HT29 cells compared with that in the other cell lines. All the 6 cell lines were found to have positive DKK1 expression by immunocytochemical staining.
  
  
  CONCLUSIONS
  DKK1 shows differential expression patterns among the 6 human colon carcinoma cell lines.
authors.affiliation: Department of Pathology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. abcdyuqian@163.com
authors.forename: Qian
authors.initials: Q
authors.lastname: Yu
authors.forename: Lei
authors.initials: L
authors.lastname: Yang
authors.forename: Yanqing
authors.initials: Y
authors.lastname: Ding
authors.forename: Zhen
authors.initials: Z
authors.lastname: Zhao
authors.forename: Huiping
authors.initials: H
authors.lastname: Liu
mesh_terms: D015153:Blotting, Western; D045744:Cell Line, Tumor; D003110:Colonic Neoplasms / Q000378:metabolism* / Q000473:pathology*; D045325:HCT116 Cells; D019073:HT29 Cells; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction
chemical_list: C501126:DKK1 protein, human; D036341:Intercellular Signaling Peptides and Proteins