title: Characterization of total phenolic constituents from the stems of Spatholobus suberectus using LC-DAD-MS(n) and their inhibitory effect on human neutrophil elastase activity.
abstract: Spatholobus suberectus Dunn, belonging to the legume family (Fabaceae), has been used as a Traditional Chinese Medicine for the treatment of anemia, menoxenia and rheumatism. A limited number of studies report that various types of flavonoids are the main characteristic constituents of this herb. We have now found that S. suberectus contains about 2% phenolic components and characterized the major phenolic components as homogeneous B-type procyanidin conjugates using a liquid chromatography with diode-array detection-ESI mass spectrometry (LC-DAD/ESI-MS) method. This is the first report on occurrence of most B-type procyanidins in this herb. Moreover, the total phenolics extract was assayed for inhibitory activity on human neutrophil elastase and its IC₅₀ was found to be 1.33 μg/mL.
authors.affiliation: School of Pharmacy, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
authors.forename: Youwu
authors.initials: Y
authors.lastname: Huang
authors.forename: Liang
authors.initials: L
authors.lastname: Chen
authors.forename: Li
authors.initials: L
authors.lastname: Feng
authors.forename: Fujiang
authors.initials: F
authors.lastname: Guo
authors.forename: Yiming
authors.initials: Y
authors.lastname: Li
mesh_terms: D002853:Chromatography, Liquid; D007887:Fabaceae / Q000378:metabolism*; D005419:Flavonoids / Q000032:analysis / Q000737:chemistry; D006801:Humans; D019272:Leukocyte Elastase / Q000037:antagonists & inhibitors* / Q000187:drug effects; D013058:Mass Spectrometry; D008516:Medicine, Chinese Traditional; D009504:Neutrophils / Q000201:enzymology; D010636:Phenols / Q000032:analysis; D018547:Plant Stems / Q000737:chemistry / Q000378:metabolism*; D044945:Proanthocyanidins / Q000032:analysis* / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D005419:Flavonoids; D010636:Phenols; D044945:Proanthocyanidins; D019272:Leukocyte Elastase