title: [The structure of the complexes of DNA with nonhistone chromosomal protein HMGB1 and the histone H1 in the presence of manganese ions. II. Vibrational circular dichroism spectroscopy].
abstract: Complexes of DNA with nonhistone chromosomal protein HMGB1 and histone H1 in the presence of manganese ions were studied using absorption and circular dichroism spectroscopy in infrared region. It was shown that the approach provides good results for solutions containing large particles, which cause light scattering in UV region. It was also shown that the manganese ions are able to coordinate to the chemical groups of DNA as well as to the carboxylic amino acid residues of the protein HMGB1. The latter stimulates DNA condensation and slightly weakens DNA-protein interactions in the complex.
authors.forename: A M
authors.initials: AM
authors.lastname: Polianichko
authors.forename: V I
authors.initials: VI
authors.lastname: Vorob'ev
authors.forename: E V
authors.initials: EV
authors.lastname: Chikhirzhina
mesh_terms: D000818:Animals; D002417:Cattle; D002942:Circular Dichroism; D004247:DNA / Q000737:chemistry*; D024243:HMGB1 Protein / Q000737:chemistry*; D006657:Histones / Q000737:chemistry*; D008345:Manganese / Q000737:chemistry; D009690:Nucleic Acid Conformation; D012996:Solutions / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet; D013057:Spectrum Analysis
chemical_list: D024243:HMGB1 Protein; D006657:Histones; D012996:Solutions; D008345:Manganese; D004247:DNA