title: Simultaneous monitoring of superoxides and intracellular calcium ions in neutrophils by chemiluminescence and fluorescence: evaluation of action mechanisms of bioactive compounds in foods.
abstract: We have developed a measuring system for simultaneous monitoring of chemiluminescence and fluorescence, which indicate respectively, (i) generation of superoxide anion radicals (O2(-•)) and (ii) change in the intracellular calcium ion concentration ([Ca(2+)]i) of neutrophils triggered by the mechanism of innate immune response. We applied this measuring system for establishing a method to distinguish between anti-inflammatory actions and antioxidant actions caused by bioactive compounds. We evaluated anti-inflammatory agents (zinc ion [Zn(2+)] and ibuprofen) and antioxidants (superoxide dismutase [SOD] and ascorbic acid). It was shown that ibuprofen and Zn(2+) were anti-inflammatory while SOD and ascorbic acid were anti-oxidative. We conclude that it is possible to determine the mechanism of action of bioactive compounds using this method.
authors.affiliation: Central Research Laboratory, Hamamatsu Photonics K.K., 5000 Hirakuchi, Hamakita-ku, Hamamatsu 434-8601, Japan. kimiko-k@crl.hpk.co.jp
authors.forename: Kimiko
authors.initials: K
authors.lastname: Kazumura
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Sato
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Satozono
authors.forename: Takashi
authors.initials: T
authors.lastname: Koike
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Tsuchiya
authors.forename: Mitsuo
authors.initials: M
authors.lastname: Hiramatsu
authors.forename: Masakazu
authors.initials: M
authors.lastname: Katsumata
authors.forename: Shigetoshi
authors.initials: S
authors.lastname: Okazaki
mesh_terms: D000893:Anti-Inflammatory Agents / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology; D000975:Antioxidants / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology; D001205:Ascorbic Acid / Q000378:metabolism; D002118:Calcium / Q000032:analysis* / Q000097:blood / Q000737:chemistry; D045744:Cell Line, Tumor; D005502:Food; D005504:Food Analysis / Q000379:methods*; D018922:HL-60 Cells; D006801:Humans; D007477:Ions / Q000737:chemistry / Q000378:metabolism; D008163:Luminescent Measurements / Q000379:methods; D009504:Neutrophils / Q000737:chemistry*; D010084:Oxidation-Reduction; D013050:Spectrometry, Fluorescence / Q000379:methods; D013482:Superoxide Dismutase / Q000378:metabolism; D013481:Superoxides / Q000032:analysis* / Q000097:blood / Q000737:chemistry; D015032:Zinc / Q000378:metabolism
chemical_list: D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D007477:Ions; D013481:Superoxides; D013482:Superoxide Dismutase; D015032:Zinc; D001205:Ascorbic Acid; D002118:Calcium
keywords: Anti-inflammatory action; Antioxidant action; Innate immune response; Intracellular calcium ion concentration; Superoxide anion radical