title: Inverting hydrolases and their use in enantioconvergent biotransformations.
abstract: Owing to the more abundant occurrence of racemic compounds compared to prochiral or meso forms, most enantiomerically pure products are obtained via racemate resolution. This review summarizes (chemo)enzymatic enantioconvergent processes based on the use of hydrolytic enzymes, which are able to invert a stereocenter during catalysis that can overcome the 50%-yield limitation of kinetic resolution. Recent developments are presented in the fields of inverting or retaining sulfatases, epoxide hydrolases and dehalogenases, which allow the production of secondary alcohols or vicinal diols at a 100% theoretical yield from a racemate via enantioconvergent processes.
authors.affiliation: Department of Chemistry, Organic and Bioorganic Chemistry, University of Graz, Heinrichstrasse 28, A-8010 Graz, Austria.
authors.forename: Markus
authors.initials: M
authors.lastname: Schober
authors.forename: Kurt
authors.initials: K
authors.lastname: Faber
mesh_terms: D001709:Biotechnology / Q000379:methods*; D001711:Biotransformation; D006867:Hydrolases / Q000378:metabolism*; D013237:Stereoisomerism
chemical_list: D006867:Hydrolases
keywords: dehalogenase; deracemization; enantioconvergent process; epoxide hydrolase; inversion; retention; sulfatase