title: Staphylococcus epidermidis biofilm formation and structural organization on different types of intraocular lenses under in vitro flow conditions.
abstract: OBJECTIVE
  To compare the adherence and structural organization of Staphylococcus epidermidis biofilm on intraocular lenses (IOLs).
  
  
  METHODS
  IOLs made of 3 different biomaterials [polymethyl methacrylate (PMMA), hydrophilic acrylic or hydrophobic acrylic] were incubated into an S. epidermidis bacterial solution. Scanning electron microscopy was used to count the bound bacteria and to analyze the structural biofilm architecture.
  
  
  RESULTS
  After 4-6 h of incubation, adherence was statistically weakest on the hydrophilic acrylic polymer. On the hydrophobic acrylic material, the bacterial cells tended to cover the substratum in a horizontal spread in a continuous monolayer. On the hydrophilic acrylic material or on the PMMA material bacterial cells tended to form only few, small scattered cell clusters.
  
  
  CONCLUSIONS
  The data suggest that the pattern of S. epidermidis adhesion varies with the IOL biomaterial. Hydrophobic IOLs seem to be more permissive to S. epidermidis adhesion.
authors.affiliation: Department of Ophthalmology, Saint Roch University Hospital, Nice, France.
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Baillif
authors.forename: Frank
authors.initials: F
authors.lastname: Leduff
authors.forename: Daniel J
authors.initials: DJ
authors.lastname: Hartmann
authors.forename: Laurent
authors.initials: L
authors.lastname: Kodjikian
mesh_terms: D000180:Acrylic Resins; D001422:Bacterial Adhesion / Q000502:physiology*; D001431:Bacteriological Techniques; D018441:Biofilms / Q000254:growth & development*; D007910:Lenses, Intraocular / Q000382:microbiology*; D008855:Microscopy, Electron, Scanning; D019904:Polymethyl Methacrylate; D013212:Staphylococcus epidermidis / Q000502:physiology* / Q000648:ultrastructure
chemical_list: D000180:Acrylic Resins; D019904:Polymethyl Methacrylate