title: Neuroprotective effect of quercetin in ectoenzymes and acetylcholinesterase activities in cerebral cortex synaptosomes of cadmium-exposed rats.
abstract: This study investigated the effect of quercetin on nucleoside triphosphate diphosphohydrolase (NTP-Dase), 50-nucleotidase, adenosine deaminase (ADA), and acetylcholinesterase (AChE) activities in synaptosomes from cerebral cortex of adult rats exposed to cadmium (Cd). Rats were exposed to Cd (2.5 mg/Kg) and quercetin (5, 25 or 50 mg/Kg) by gavage for 45 days. Rats were randomly divided into eight groups (n = 8-10): saline/ethanol, saline/Querc 5 mg/kg, saline/Querc 25 mg/kg, saline/Querc 50 mg/kg, Cd/ethanol, Cd/Querc 5 mg/kg, Cd/Querc 25 mg/kg, and Cd/Querc 50 mg/kg. Results demonstrated that AChE activity increased in the Cd/ethanol group when compared to saline/ethanol group. Treatment with quercetin prevented the increase in AChE activity when compared to Cd/ethanol group. Quercetin treatment prevented the cadmium-induced increase in NTPDase, 5-nucleotidase, and ADA activities in Cd/ethanol group when compared to saline/ethanol group. Our data showed that quercetin have a protector effect against Cd intoxication. This way, is a promising candidate among the flavonoids to be investigated as a therapeutic agent to attenuate neurological disorders associated with Cd intoxication.
authors.affiliation: Department of Chemistry, Center of Natural Exact Sciences, Federal University of Santa Maria, Santa Maria, RS, Brazil. faha.biomed@hotmail.com
authors.forename: Fátima Husein
authors.initials: FH
authors.lastname: Abdalla
authors.forename: Andréia Machado
authors.initials: AM
authors.lastname: Cardoso
authors.forename: Luciane Belmonte
authors.initials: LB
authors.lastname: Pereira
authors.forename: Roberta
authors.initials: R
authors.lastname: Schmatz
authors.forename: Jamile Fabbrin
authors.initials: JF
authors.lastname: Gonçalves
authors.forename: Naiara
authors.initials: N
authors.lastname: Stefanello
authors.forename: Amanda Maino
authors.initials: AM
authors.lastname: Fiorenza
authors.forename: Jessié Martins
authors.initials: JM
authors.lastname: Gutierres
authors.forename: Jonas Daci da Silva
authors.initials: JD
authors.lastname: Serres
authors.forename: Daniela
authors.initials: D
authors.lastname: Zanini
authors.forename: Victor Camera
authors.initials: VC
authors.lastname: Pimentel
authors.forename: Juliano Marchi
authors.initials: JM
authors.lastname: Vieira
authors.forename: Maria Rosa Chitolina
authors.initials: MR
authors.lastname: Schetinger
authors.forename: Vera Maria
authors.initials: VM
authors.lastname: Morsch
authors.forename: Cinthia Melazzo
authors.initials: CM
authors.lastname: Mazzanti
mesh_terms: D015720:5'-Nucleotidase / Q000378:metabolism*; D000110:Acetylcholinesterase / Q000378:metabolism*; D000243:Adenosine Deaminase / Q000378:metabolism; D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D001081:Apyrase / Q000378:metabolism; D002104:Cadmium / Q000633:toxicity*; D002540:Cerebral Cortex / Q000187:drug effects / Q000201:enzymology* / Q000473:pathology; D006868:Hydrolysis; D008297:Male; D018696:Neuroprotective Agents / Q000494:pharmacology*; D009711:Nucleotides / Q000378:metabolism; D011794:Quercetin / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D013574:Synaptosomes / Q000187:drug effects / Q000201:enzymology* / Q000473:pathology
chemical_list: D015703:Antigens, CD; D018696:Neuroprotective Agents; D009711:Nucleotides; D002104:Cadmium; D011794:Quercetin; D000110:Acetylcholinesterase; D015720:5'-Nucleotidase; D000243:Adenosine Deaminase; D001081:Apyrase; C067869:CD39 antigen