title: Synthesis of enantiopure fluorohydrins using alcohol dehydrogenases at high substrate concentrations.
abstract: The use of purified and overexpressed alcohol dehydrogenases to synthesize enantiopure fluorinated alcohols is shown. When the bioreductions were performed with ADH-A from Rhodococcus ruber overexpressed in E. coli, no external cofactor was necessary to obtain the enantiopure (R)-derivatives. Employing Lactobacillus brevis ADH, it was possible to achieve the synthesis of enantiopure (S)-fluorohydrins at a 0.5 M substrate concentration. Furthermore, due to the activated character of these substrates, a huge excess of the hydrogen donor was not necessary.
authors.affiliation: Departamento de Química Orgánica e Inorgánica, University of Oviedo, C/Julián Clavería 8, 33006 Oviedo, Spain.
authors.forename: Wioleta
authors.initials: W
authors.lastname: Borzęcka
authors.forename: Iván
authors.initials: I
authors.lastname: Lavandera
authors.forename: Vicente
authors.initials: V
authors.lastname: Gotor
mesh_terms: D000426:Alcohol Dehydrogenase / Q000378:metabolism*; D006845:Hydrocarbons, Fluorinated / Q000737:chemistry / Q000378:metabolism*; D015394:Molecular Structure; D012240:Rhodococcus / Q000201:enzymology*
chemical_list: D006845:Hydrocarbons, Fluorinated; D000426:Alcohol Dehydrogenase