title: Methods to study the proliferation and differentiation of cardiac side population (CSP) cells.
abstract: Investigation of cardiac progenitor cell proliferation and differentiation is essential for both the basic understanding of progenitor cell biology as well as the development of cellular therapeutics for tissue regeneration. Herein, we describe techniques used for the analysis of CSP cell proliferation, cell cycle status, and cardiomyogenic differentiation.
authors.affiliation: Cardiovascular Division, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA.
authors.forename: Konstantina-Ioanna
authors.initials: KI
authors.lastname: Sereti
authors.forename: Angelos
authors.initials: A
authors.lastname: Oikonomopoulos
authors.forename: Kazumasa
authors.initials: K
authors.lastname: Unno
authors.forename: Ronglih
authors.initials: R
authors.lastname: Liao
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002453:Cell Cycle; D002454:Cell Differentiation; D049109:Cell Proliferation; D002469:Cell Separation / Q000379:methods*; D002478:Cells, Cultured; D005434:Flow Cytometry; D032383:Myocytes, Cardiac / Q000166:cytology* / Q000378:metabolism; D051381:Rats; D058985:Side-Population Cells / Q000166:cytology* / Q000378:metabolism; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism