title: Anti-oxidant activity of holo- and apo-c-phycocyanin and their protective effects on human erythrocytes.
abstract: This study was conducted to investigate the anti-oxidant activity of the recombinant apo-c-phycocyanin (c-PC) β-subunit compared to native c-PC purified from Spirulina sp. The gene encoding the β-subunit of c-PC was successfully cloned and expressed in Escherichia coli. The anti-oxidant capacities of recombinant apo-c-PC(β) and native c-PC were evaluated by measuring their Trolox equivalent antioxidant capacities and examining their protective effects on erythrocytes from normal and homozygous haemoglobin E individuals against peroxyl radicals and hydrogen peroxide. The results demonstrated that the anti-oxidant capacities are native c-PC≫Trolox>recombinant apo-c-PC(β). Both anti-oxidant proteins can potentially protect erythrocytes from oxidative damage. Expression of c-PC in bacteria reduces the cost and time for protein production, and the recombinant protein could be further developed to obtain a more efficient protein for therapeutic purposes.
authors.affiliation: Graduate Program of Clinical Haematology Sciences, Department of Clinical Microscopy, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok 10330, Thailand.
authors.forename: Pornthip
authors.initials: P
authors.lastname: Pleonsil
authors.forename: Suphan
authors.initials: S
authors.lastname: Soogarun
authors.forename: Yaneenart
authors.initials: Y
authors.lastname: Suwanwong
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000235:genetics / Q000302:isolation & purification / Q000494:pharmacology; D003001:Cloning, Molecular; D000458:Cyanobacteria / Q000378:metabolism; D004912:Erythrocytes / Q000187:drug effects*; D016166:Free Radical Scavengers / Q000494:pharmacology; D015870:Gene Expression; D006801:Humans; D018384:Oxidative Stress / Q000187:drug effects; D010798:Phycocyanin / Q000235:genetics / Q000302:isolation & purification / Q000494:pharmacology*; D021122:Protein Subunits; D011994:Recombinant Proteins / Q000235:genetics / Q000302:isolation & purification / Q000494:pharmacology
chemical_list: D000975:Antioxidants; D001426:Bacterial Proteins; D016166:Free Radical Scavengers; D021122:Protein Subunits; D011994:Recombinant Proteins; D010798:Phycocyanin
keywords: Anti-oxidant; Erythrocyte; Haemoglobin E; Phycocyanin; Recombinant protein