title: Metabolite profiling and quantification of phytochemicals in potato extracts using ultra-high-performance liquid chromatography-mass spectrometry.
abstract: BACKGROUND
  Potatoes contain a diverse range of phytochemicals which have been suggested to have health benefits. Metabolite profiling and quantification were conducted on plant extracts made from a white potato cultivar and 'Urenika', a purple potato cultivar traditionally consumed by New Zealand Maori. There is limited published information regarding the metabolite profile of Solanum tuberosum cultivar 'Urenika'.
  
  
  RESULTS
  Using ultra-high- performance liquid chromatography-mass spectrometry (UHPLC-MS), a total of 31 compounds were identified and quantified in the potato extracts. The majority of the compounds were identified for the first time in 'Urenika'. These compounds include several types of anthocyanins, hydroxycinnamic acid (HCA) derivatives, and hydroxycinnamic amides (HCAA). Six classes of compounds, namely organic acids, amino acids, HCA, HCAA, flavonols and glycoalkaloids, were present in both extracts but quantities varied between the two extracts.
  
  
  CONCLUSIONS
  The unknown plant metabolites in both potato extracts were assigned with molecular formulae and identified with high confidence. Quantification of the metabolites was achieved using a number of appropriate standards. High-resolution mass spectrometry data critical for accurate identification of unknown phytochemicals were achieved and could be added to potato or plant metabolomic database.
authors.affiliation: Institute of Food, Nutrition and Human Health (IFNHH), Massey University, Private Bag, 11222, Palmerston North, New Zealand.
authors.forename: Esther Swee Lan
authors.initials: ES
authors.lastname: Chong
authors.forename: Tony K
authors.initials: TK
authors.lastname: McGhie
authors.forename: Julian A
authors.initials: JA
authors.lastname: Heyes
authors.forename: Kathryn M
authors.initials: KM
authors.lastname: Stowell
mesh_terms: D000470:Alkaloids / Q000032:analysis; D000596:Amino Acids / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D003373:Coumaric Acids / Q000032:analysis; D005419:Flavonoids / Q000032:analysis; D013058:Mass Spectrometry; D055442:Metabolome; D009520:New Zealand; D064209:Phytochemicals / Q000032:analysis*; D010936:Plant Extracts / Q000737:chemistry*; D035281:Plant Tubers / Q000737:chemistry*; D011198:Solanum tuberosum / Q000737:chemistry* / Q000145:classification / Q000378:metabolism; D013045:Species Specificity
chemical_list: D000470:Alkaloids; D000596:Amino Acids; D003373:Coumaric Acids; D005419:Flavonoids; D064209:Phytochemicals; D010936:Plant Extracts
keywords: LCMS; Urenika; mass spectrometry; metabolite; phytochemical; potato