title: OsSUV3 dual helicase functions in salinity stress tolerance by maintaining photosynthesis and antioxidant machinery in rice (Oryza sativa L. cv. IR64).
abstract: To overcome the salinity-induced loss of crop yield, a salinity-tolerant trait is required. The SUV3 helicase is involved in the regulation of RNA surveillance and turnover in mitochondria, but the helicase activity of plant SUV3 and its role in abiotic stress tolerance have not been reported so far. Here we report that the Oryza sativa (rice) SUV3 protein exhibits DNA and RNA helicase, and ATPase activities. Furthermore, we report that SUV3 is induced in rice seedlings in response to high levels of salt. Its expression, driven by a constitutive cauliflower mosaic virus 35S promoter in IR64 transgenic rice plants, confers salinity tolerance. The T1 and T2 sense transgenic lines showed tolerance to high salinity and fully matured without any loss in yields. The T2 transgenic lines also showed tolerance to drought stress. These results suggest that the introduced trait is functional and stable in transgenic rice plants. The rice SUV3 sense transgenic lines showed lesser lipid peroxidation, electrolyte leakage and H2 O2 production, along with higher activities of antioxidant enzymes under salinity stress, as compared with wild type, vector control and antisense transgenic lines. These results suggest the existence of an efficient antioxidant defence system to cope with salinity-induced oxidative damage. Overall, this study reports that plant SUV3 exhibits DNA and RNA helicase and ATPase activities, and provides direct evidence of its function in imparting salinity stress tolerance without yield loss. The possible mechanism could be that OsSUV3 helicase functions in salinity stress tolerance by improving photosynthesis and antioxidant machinery in transgenic rice.
authors.affiliation: International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi, 110067, India.
authors.forename: Narendra
authors.initials: N
authors.lastname: Tuteja
authors.forename: Ranjan Kumar
authors.initials: RK
authors.lastname: Sahoo
authors.forename: Bharti
authors.initials: B
authors.lastname: Garg
authors.forename: Renu
authors.initials: R
authors.lastname: Tuteja
mesh_terms: D000975:Antioxidants / Q000378:metabolism; D053487:DEAD-box RNA Helicases / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D004265:DNA Helicases / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D055864:Droughts; D018506:Gene Expression Regulation, Plant; D015227:Lipid Peroxidation; D012275:Oryza / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development / Q000502:physiology; D018384:Oxidative Stress; D010788:Photosynthesis; D010940:Plant Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D030821:Plants, Genetically Modified; D020365:RNA Helicases / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D054712:Salinity; D055049:Salt Tolerance; D036226:Seedlings / Q000201:enzymology / Q000235:genetics / Q000254:growth & development / Q000502:physiology; D012965:Sodium Chloride / Q000494:pharmacology*; D013312:Stress, Physiological
chemical_list: D000975:Antioxidants; D010940:Plant Proteins; D012965:Sodium Chloride; D004265:DNA Helicases; D053487:DEAD-box RNA Helicases; D020365:RNA Helicases
keywords: ATPase; DNA and RNA helicase; Oryza sativa; SUV3; antioxidant; salinity stress; unwinding