title: Composition of long chain polyunsaturated fatty acids (LC-PUFAs) in different encephalic regions and its association with behavior in spontaneous hypertensive rat (SHR).
abstract: Long chain polyunsaturated fatty acids (LC-PUFAs) are hypothesized to play an important role in attention deficit/hyperactivity disorder (ADHD). This study evaluated LC-PUFAs composition in different encephalic regions by gas chromatography and its association with behavior on the attentional set-shifting task, open field test and the Morris water maze of spontaneous hypertensive rat (SHR)-a genetic animal model of ADHD. In behavioral tests, the SHRs exhibited deficiencies in attentional set-shifting, autonomic activities and spatial learning and memory. In all the studied encephalic regions, we observed higher concentration of docosahexaenoic acid (DHA) and arachidonic acid (AA) and higher AA/DHA ratio in the SHRs compared with the Wistar-Kyoto (WKY) and Sprague-Dawley (SD) rats (p<0.01), which was associated with abnormal behavior in the SHRs. This study provided an appropriate animal model for study on the relationship between LC-PUFAs and ADHD. Our results prove abnormal neurobehaviour associated with imbalance of AA/DHA ratio and highlights the significance of normal AA/DHA ratio in behavior.
authors.affiliation: Department of Pediatrics, Qilu Hospital, Shandong University, Jinan 250012, PR China. xinercah@163.com
authors.forename: Ai-hua
authors.initials: AH
authors.lastname: Cao
authors.forename: Lin
authors.initials: L
authors.lastname: Yu
authors.forename: Yu-wei
authors.initials: YW
authors.lastname: Wang
authors.forename: Gui-ju
authors.initials: GJ
authors.lastname: Wang
authors.forename: Ge-fei
authors.initials: GF
authors.lastname: Lei
mesh_terms: D000818:Animals; D016718:Arachidonic Acid / Q000032:analysis; D001289:Attention Deficit Disorder with Hyperactivity / Q000209:etiology*; D001522:Behavior, Animal; D001923:Brain Chemistry; D004281:Docosahexaenoic Acids / Q000032:analysis; D005231:Fatty Acids, Unsaturated / Q000032:analysis*; D019787:Linoleic Acid / Q000032:analysis; D008297:Male; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY; D017207:Rats, Sprague-Dawley; D017962:alpha-Linolenic Acid / Q000032:analysis
chemical_list: D005231:Fatty Acids, Unsaturated; D017962:alpha-Linolenic Acid; D004281:Docosahexaenoic Acids; D016718:Arachidonic Acid; D019787:Linoleic Acid
keywords: Attention deficit/hyperactivity disorder; Behavioral tests; Different encephalic regions; High AA/DHA ratio; Long chain polyunsaturated fatty acids (LC-PUFAs); Spontaneous hypertensive rat