title: [The directed modification of Escherichia coli MG1655 to obtain histidine-producing mutants].
abstract: Strain MG 1655+hisGr hisL'-Delta, purR, which produces histidine with a weight yield of approximately 12% from glucose, was constructed through directed chromosomal modifications of the laboratory Escherichia coli strain MG 1655+, which has a known genome sequence. A feedback-resistant ATP-phosphoribosyl transferase encoded by the mutant hisGr (E271 K) was the main determinant of histidine production. A further increase in histidine production was achieved by the expression enhance of a mutant his operon containing hisGr through the deleting attenuator region (hisL'-Delta). An increase in the expression of the wildtype his operon did not result in histidine accumulation. Deletion of the transcriptional regulator gene purR increased the biomass produced and maintained the level of histidine production per cell under the fermentation conditions used.
authors.forename: V G
authors.initials: VG
authors.lastname: Doroshenko
authors.forename: A O
authors.initials: AO
authors.lastname: Lobanov
authors.forename: E A
authors.initials: EA
authors.lastname: Fedorina
mesh_terms: D001276:ATP Phosphoribosyltransferase / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D018533:Biomass; D004926:Escherichia coli / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000235:genetics; D005285:Fermentation; D015964:Gene Expression Regulation, Bacterial; D005947:Glucose / Q000378:metabolism; D006639:Histidine / Q000096:biosynthesis*; D060847:Metabolic Engineering; D008969:Molecular Sequence Data; D009154:Mutation; D009876:Operon; D020543:Proteome / Q000235:genetics* / Q000378:metabolism; D012097:Repressor Proteins / Q000172:deficiency / Q000235:genetics; D017384:Sequence Deletion
chemical_list: D029968:Escherichia coli Proteins; D020543:Proteome; C489317:PurR protein, E coli; D012097:Repressor Proteins; D006639:Histidine; D001276:ATP Phosphoribosyltransferase; D005947:Glucose