title: Dietary and ontogenic regulation of fatty acid desaturase and elongase expression in broiler chickens.
abstract: Effects of diet and ontogeny on the expression of fatty acid desaturases and elongases were examined in broiler chickens. In Study 1, 120 day-old male chicks received one of six diets with LA:ALA ranging from 46:4 to 16:34, for 33 days. Total n-6 PUFA decreased, and n-3 PUFA increased in response to a decrease in the dietary LA:ALA. FADS1, FADS2, ELOVL2 and ELOVL5 mRNAs were highest (P<0.05) in birds fed lower LA:ALA diets. In Study 2, 60 day-old male chicks were fed a basal diet, and liver samples were collected on day of hatch, and on days 2, 7, 14, 21 and 35 post-hatch. Total n-6 and n-3 PUFA increased (P<0.01) from days 7 to days 21. FADS1, FADS2 and ELOVL2 mRNAs generally increased (P<0.01) with age. These findings provide evidence for the dietary and developmental regulation of PUFA metabolism in broiler chickens.
authors.affiliation: Department of Animal Science, University of Manitoba, Winnipeg, MB, Canada R3T 2N2.
authors.forename: M
authors.initials: M
authors.lastname: Jing
authors.forename: N
authors.initials: N
authors.lastname: Gakhar
authors.forename: R A
authors.initials: RA
authors.lastname: Gibson
authors.forename: J D
authors.initials: JD
authors.lastname: House
mesh_terms: D000123:Acetyltransferases / Q000378:metabolism*; D000818:Animals; D002645:Chickens; D044943:Fatty Acid Desaturases / Q000378:metabolism*; D005231:Fatty Acids, Unsaturated / Q000378:metabolism*; D008099:Liver / Q000201:enzymology / Q000378:metabolism; D008297:Male
chemical_list: D005231:Fatty Acids, Unsaturated; D044943:Fatty Acid Desaturases; D000123:Acetyltransferases
keywords: Desaturation; Development; Diet; Elongation; Polyunsaturated fatty acids; Post-hatching