title: Identification of wheat gene Sr35 that confers resistance to Ug99 stem rust race group.
abstract: Wheat stem rust, caused by Puccinia graminis f. sp. tritici (Pgt), is a devastating disease that can cause severe yield losses. A previously uncharacterized Pgt race, designated Ug99, has overcome most of the widely used resistance genes and is threatening major wheat production areas. Here, we demonstrate that the Sr35 gene from Triticum monococcum is a coiled-coil, nucleotide-binding, leucine-rich repeat gene that confers near immunity to Ug99 and related races. This gene is absent in the A-genome diploid donor and in polyploid wheat but is effective when transferred from T. monococcum to polyploid wheat. The cloning of Sr35 opens the door to the use of biotechnological approaches to control this devastating disease and to analyses of the molecular interactions that define the wheat-rust pathosystem.
authors.affiliation: Kansas State University, Dept. Plant Pathology, Manhattan, KS 66506.
authors.forename: Cyrille
authors.initials: C
authors.lastname: Saintenac
authors.affiliation: University of California, Davis Dept. Plant Sciences, Davis, CA 95616.
authors.forename: Wenjun
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Kansas State University, Dept. Plant Pathology, Manhattan, KS 66506.
authors.forename: Andres
authors.initials: A
authors.lastname: Salcedo
authors.affiliation: USDA-ARS Cereal Disease Laboratory, St. Paul, MN 55108.
authors.forename: Matthew N
authors.initials: MN
authors.lastname: Rouse
authors.affiliation: Kansas State University, Dept. Plant Pathology, Manhattan, KS 66506.
authors.forename: Harold N
authors.initials: HN
authors.lastname: Trick
authors.affiliation: Kansas State University, Dept. Plant Pathology, Manhattan, KS 66506.
authors.forename: Eduard
authors.initials: E
authors.lastname: Akhunov
authors.affiliation: University of California, Davis Dept. Plant Sciences, Davis, CA 95616.|Howard Hughes Medical Institute, Chevy Chase, MD 20815.
authors.forename: Jorge
authors.initials: J
authors.lastname: Dubcovsky
mesh_terms: D017398:Alternative Splicing; D000595:Amino Acid Sequence; D001487:Basidiomycota / Q000472:pathogenicity; D003001:Cloning, Molecular; D060467:Disease Resistance / Q000235:genetics; D017343:Genes, Plant; D006239:Haplotypes; D058977:Molecular Sequence Annotation; D008969:Molecular Sequence Data; D009154:Mutation; D010802:Phylogeny; D010935:Plant Diseases / Q000235:genetics / Q000276:immunology* / Q000382:microbiology; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics; D018547:Plant Stems / Q000382:microbiology; D030821:Plants, Genetically Modified; D020641:Polymorphism, Single Nucleotide; D011123:Polyploidy; D017422:Sequence Analysis, DNA; D014908:Triticum / Q000235:genetics* / Q000276:immunology / Q000382:microbiology
chemical_list: D010940:Plant Proteins