title: Screening and determination for potential α-glucosidase inhibitory constituents from Dalbergia odorifera T. Chen using ultrafiltration-LC/ESI-MS(n).
abstract: In the present study, it was demonstrated that ethyl acetate soluble fraction partitioned from heartwood of Dalbergia odorifera T. Chen (HEF) had a remarkable inhibitory effect on α-glucosidase. Therefore HEF was selected as a starting material for screening the potential α-glucosidase inhibitors using ultrafiltration liquid chromatography/mass spectrometry (UF-LC/MS). Twenty-six compounds were identified with analysis of LC/MS. UF assay indicated that 18 compositions might be α-glucosidase inhibitors in HEF; eight of them were estimated for their α-glucosidase inhibitory activity, and the results showed that (2S)-liquiritigenin, (2S)-4',6-dihydroxy- 7-methoxyflavanone and isoliquiritigenin displayed obvious inhibition of yeast α-glucosidase. In addition, in order to control the quality of HEF, the content of five compounds in HEF was simultaneously determined for the first time. These results provide an important theoretical base for the further application of HEF to treat type 2 diabetes in the clinic and development of natural α-glucosidase inhibitors with low toxicity.
authors.affiliation: College of Pharmacy, Jilin University, Xinmin Campus, 130021, Changchun, Jilin, People's Republic of China.
authors.forename: Chunfang
authors.initials: C
authors.lastname: Zhao
authors.forename: Yongqiang
authors.initials: Y
authors.lastname: Liu
authors.forename: Dengli
authors.initials: D
authors.lastname: Cong
authors.forename: Hui
authors.initials: H
authors.lastname: Zhang
authors.forename: Jiajia
authors.initials: J
authors.lastname: Yu
authors.forename: Ying
authors.initials: Y
authors.lastname: Jiang
authors.forename: Xiuyue
authors.initials: X
authors.lastname: Cui
authors.forename: Jiaming
authors.initials: J
authors.lastname: Sun
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods*; D035782:Dalbergia / Q000737:chemistry*; D004791:Enzyme Inhibitors / Q000032:analysis* / Q000302:isolation & purification / Q000378:metabolism; D065089:Glycoside Hydrolase Inhibitors; D057230:Limit of Detection; D016014:Linear Models; D010936:Plant Extracts / Q000737:chemistry*; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D014462:Ultrafiltration / Q000379:methods*; D014934:Wood / Q000737:chemistry
chemical_list: D004791:Enzyme Inhibitors; D065089:Glycoside Hydrolase Inhibitors; D010936:Plant Extracts
keywords: Dalbergia odorifera T. Chen; ultrafiltration-LC-MS; α-glucosidase inhibitor