title: Occurrence and analysis of rare cfiA-bft doubly positive Bacteroides fragilis strains.
abstract: We detected four cfiA-bft1 doubly positive Bacteroides fragilis strains out of 486 B. fragilis isolates analyzed for antibiotic susceptibilities and antibiotic resistance genes from a recent pan-European survey. The prevalence of the enterotoxin bft genes was roughly equal among cfiA-negative and -positive B. fragilis strains. We also demonstrated that the cfiA-bft doubly positive strains had the most common B. fragilis genomic pattern (I.1.). Thus we concluded that the bft-carrying CTn86 conjugative transposons are mobile accounting for this unexpected simultaneous occurrence of the cfiA and bft genes.
authors.affiliation: Institute of Clinical Microbiology, Albert Szent-Györgyi Clinical Center, Faculty of Medicine, University of Szeged, Semmelweis 6, H-6725 Szeged, Hungary. Electronic address: soki.jozsef@med.u-szeged.hu.
authors.forename: József
authors.initials: J
authors.lastname: Sóki
authors.forename: Zsuzsa
authors.initials: Z
authors.lastname: Eitel
authors.forename: Gabriella
authors.initials: G
authors.lastname: Terhes
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Nagy
authors.forename: Edit
authors.initials: E
authors.lastname: Urbán
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics*; D001427:Bacterial Toxins / Q000235:genetics*; D001442:Bacteroides Infections / Q000382:microbiology*; D001441:Bacteroides fragilis / Q000235:genetics* / Q000302:isolation & purification; D003227:Conjugation, Genetic; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D005060:Europe; D022761:Gene Transfer, Horizontal; D006801:Humans; D008666:Metalloendopeptidases / Q000235:genetics*; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA; D001618:beta-Lactamases / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004251:DNA Transposable Elements; D004269:DNA, Bacterial; C405031:Bacteroides fragilis toxin; D008666:Metalloendopeptidases; D001618:beta-Lactamases; C063912:carbapenemase
keywords: Bacteroides fragilis; Conjugative transposons; Enterotoxin; bft1; cfiA