title: Comparison of some biochemical properties of artichoke polyphenol oxidase entrapped in alginate-carrageenan and alginate gels.
abstract: Polyphenol oxidase (PPO, EC.1.14.18.1) isolated from artichoke (Cynara scolymus) was entrapped within alginate and alginate+ carrageenan beads, and the catecholase and cresolase activities of both entrapped enzymes were determined. Some properties of these immobilized enzymes such as optimum pH and temperature, kinetic parameters (Km and Vmax), thermal, and storage stability were determined and compared to each other. The highest catecholase activity was observed in alginate gel (370 U/g bead) while the highest cresolase activity was in alginate+ carrageenan gel (90 U/g bead). For catecholase and cresolase activities, optimum pHs of alginate and alginate+ carrageenan beads were determined to be 7.0 and 4.0, respectively. Optimum temperatures for catecholase activity were determined to be 40°C for both entrapped enzymes. These values for cresolase activity were 30°C and 20°C, respectively. Immobilized artichoke PPOs greatly preserved their thermal stability which exists anyway. The catalytic efficiency value (Vmax/Km) of the alginate beads is approximately high as two-and-a-half folds of that of alginate+κ-carrageenan beads for cresolase activity. These values were very close for catecholase activity. Immobilized beads saved their both activities after 30 days of storage at 4°C.
authors.affiliation: Department of Chemistry, Faculty of Science, Trakya University , Edirne , Turkey.
authors.forename: Hulya
authors.initials: H
authors.lastname: Yagar
authors.forename: Selin
authors.initials: S
authors.lastname: Kocaturk
mesh_terms: D000464:Alginates / Q000737:chemistry*; D002351:Carrageenan / Q000737:chemistry*; D004156:Catechol Oxidase / Q000737:chemistry* / Q000378:metabolism*; D031943:Cynara scolymus / Q000201:enzymology*; D004800:Enzymes, Immobilized / Q000737:chemistry* / Q000378:metabolism*; D005782:Gels; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D013696:Temperature
chemical_list: D000464:Alginates; D004800:Enzymes, Immobilized; D005782:Gels; D006603:Hexuronic Acids; D020723:Glucuronic Acid; D002351:Carrageenan; D004156:Catechol Oxidase
keywords: alginate; artichoke; carrageenan; catecholase activity; cresolase activity; entrapment; polyphenol oxidase