title: [Optimization of conditions of quantitative evaluation of argentine hemorrhagic fever causative agent].
abstract: OBJECTIVE
  Optimization of conditions of quantitative evaluation of Argentine hemorrhagic fever causative agent.
  
  
  METHODS
  Junin virus (XJ P37 strain) was obtained from National collection of viral hemorrhagic fever causative agents of the 1st pathogenicity group of the 33rd Central Research Testing Institute. Junin virus (XJ P37 strain) culture with biological activity of 5.2 lg PFUxm(-1) was used in the experiments. Vero B, 6619-1(D) and GMK-AH-1(D) were obtained from collection of cell culture of the Research Scientific Centre of the 33rd Central Research Testing Institute. Calculation of biological activity of Junin virus during titration in cell cultures was carried out by Kerber method with modification by I.P. Ashmarin.
  
  
  RESULTS
  During incubation for 4 - 7 days after the infection of cell monolayer the determined biological activity was 4.4 - 6.4 lg PFUxml(-1); the size of the formed negative colonies--(1.5 +/- 0.5) mm.
  
  
  CONCLUSIONS
  The conditions of quantitative evaluation of Argentine hemorrhagic fever were optimized by negative colonies method (using 5 - 7 day Vero B cell culture monolayer with staining of monolayer on day 5 of secondary incubation, recording of results at day 7 after the infection).
authors.forename: S I
authors.initials: SI
authors.lastname: Syromiatnikova
authors.forename: V B
authors.initials: VB
authors.lastname: Pantiukhov
authors.forename: I V
authors.initials: IV
authors.lastname: Shatokhina
authors.forename: V A
authors.initials: VA
authors.lastname: Markin
authors.forename: A P
authors.initials: AP
authors.lastname: Pirozhkov
authors.forename: M A
authors.initials: MA
authors.lastname: Timofeev
authors.forename: T E
authors.initials: TE
authors.lastname: Sizikova
authors.forename: I G
authors.initials: IG
authors.lastname: Rumiantseva
authors.forename: S V
authors.initials: SV
authors.lastname: Borisevich
mesh_terms: D000818:Animals; D002522:Chlorocebus aethiops; D006478:Hemorrhagic Fever, American / Q000821:virology; D018051:Junin virus / Q000254:growth & development / Q000302:isolation & purification*; D014709:Vero Cells; D010948:Viral Plaque Assay / Q000379:methods*; D014779:Virus Replication