title: Enhanced butanol production by coculture of Clostridium beijerinckii and Clostridium tyrobutyricum.
abstract: Cocultures of Clostridium beijerinckii and Clostridium tyrobutyricum in free-cell and immobilized-cell fermentation modes were investigated as a means of enhancing butanol production. The immobilized fermentation was performed in a fibrous-bed bioreactor (FBB). The results demonstrated that two-strain coculture significantly enhanced butanol production, yield and volumetric productivity compared with those in pure culture with or without butyric acid. Further, continuous immobilized-cell cocultures in two FBBs using glucose, cassava starch, or cane molasses were conducted at a dilution rate of 0.144 h(-1). The butanol production (6.66 g/L), yield (0.18 g/g), and productivity (0.96 g/L/h) were obtained with cassava starch as the substrate. Meanwhile, the acetone-butanol-ethanol (ABE) yield (0.36 g/g) was the highest among all processes investigated, suggesting that this continuous coculture mode may be suitable for industrial ABE production with no need for repeated sterilization and inoculation.
authors.affiliation: School of Bioscience & Bioengineering, South China University of Technology, Guangzhou 510006, People's Republic of China.
authors.forename: Lin
authors.initials: L
authors.lastname: Li
authors.forename: Hongxia
authors.initials: H
authors.lastname: Ai
authors.forename: Shexi
authors.initials: S
authors.lastname: Zhang
authors.forename: Shuang
authors.initials: S
authors.lastname: Li
authors.forename: Zexin
authors.initials: Z
authors.lastname: Liang
authors.forename: Zhen-Qiang
authors.initials: ZQ
authors.lastname: Wu
authors.forename: Shang-Tian
authors.initials: ST
authors.lastname: Yang
authors.forename: Ju-Fang
authors.initials: JF
authors.lastname: Wang
mesh_terms: D019149:Bioreactors; D000440:Butanols / Q000378:metabolism*; D003013:Clostridium / Q000145:classification / Q000378:metabolism*; D018920:Coculture Techniques; D005285:Fermentation; D013045:Species Specificity
chemical_list: D000440:Butanols
keywords: Acetone–butanol–ethanol fermentation; Coculture; Fibrous-bed bioreactor; Free-cell fermentation; Immobilized-cell fermentation