title: New model of subconjunctival tumor development in rabbits.
abstract: Conjunctival squamous cell carcinoma (SCC) is an uncommon disease. However, SCC has recently become an important clinical problem due the identification of a significantly high incidence of SCC among a group of black African patients with AIDS. However, basic research concerning SCC, including both intraepithelial and invasive squamous neoplasia, is limited due to the lack of an ocular tumor animal model. Specifically, current ocular imaging and treatment modalities are insufficient for investigating currently available small animal models, because the conjunctival space is not comparable to that of humans. We describe the development of a reproducible model of subconjunctival squamous carcinoma in moderate-sized immunocompetent rabbits. Under optical coherence tomography guidance, 1×10⁷ VX2 carcinoma cells are inoculated into the subconjunctival space of 3 to 4-kg New Zealand white rabbits. Malignant tumor involvement developed on the subconjunctival space after an average of 1 to 2 weeks. This subconjunctival tumor model induction method will likely facilitate a broad range of investigation of subconjunctival cancer diagnostics and therapeutics.
authors.affiliation: Pukyong National University, Department of Biomedical Engineering/Interdisciplinary Program of Medical Biotechnology Application Engineering, Busan 608-737, Republic of Korea.
authors.forename: Sang Seok
authors.initials: SS
authors.lastname: Hwang
authors.forename: Jeong Eun
authors.initials: JE
authors.lastname: Kim
authors.forename: Bong-Kwon
authors.initials: BK
authors.lastname: Chun
authors.forename: Sangbong
authors.initials: S
authors.lastname: Jung
authors.forename: Eun-Kee
authors.initials: EK
authors.lastname: Park
authors.forename: Hu-Jang
authors.initials: HJ
authors.lastname: Lee
authors.forename: Mannhong
authors.initials: M
authors.lastname: Jung
authors.forename: Sang-Joon
authors.initials: SJ
authors.lastname: Lee
authors.forename: Gwang Myung
authors.initials: GM
authors.lastname: Noh
authors.forename: Rangarirai
authors.initials: R
authors.lastname: Masanganise
authors.forename: Chulho
authors.initials: C
authors.lastname: Oak
authors.forename: Yeh-Chan
authors.initials: YC
authors.lastname: Ahn
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D003228:Conjunctiva / Q000473:pathology; D003230:Conjunctival Neoplasms / Q000473:pathology*; D004195:Disease Models, Animal; D008297:Male; D009368:Neoplasm Transplantation; D011817:Rabbits; D041623:Tomography, Optical Coherence