title: Simultaneous determination of β-agonists and psychiatric drugs in feeds by LC-MS-MS.
abstract: A method was developed for the simultaneous determination of nine β-agonists (cimaterol, ractopamine, terbutaline, zilpaterol, salbutamol, clenbuterol, mabuterol, bambuterol and brombuterol) and six psychiatric drugs (diazepam, nitrazepam, oxazepam, chlorpromazine, promethazine and perphenazine) in animal feed by using solid-phase extraction (SPE) followed by liquid chromatography-tandem mass spectrometry (LC-MS-MS). Conditions were optimized for the extraction of the target analytes from animal feed and for clean-up with MCX SPE cartridges. The eluent was evaporated to dryness under nitrogen, and the residue was dissolved in a solution of acetonitrile and 1% formic acid (2:8, v/v) and analyzed by LC-MS-MS using an isotopic internal standard for quantification. Under the optimum conditions, the recovery values of the target analytes were between 70.1 and 110%, with coefficients of variation between 1.9 and 18.4%. The method was very reliable for the simultaneous determination of nine β-agonists and six psychiatric drugs in animal feed.
authors.affiliation: Institute of Quality Standards and Testing Technology for Agricultural Products, Chinese Academy of Agricultural Science, Beijing 100081.
authors.forename: D C
authors.initials: DC
authors.lastname: Suo
authors.affiliation: Institute of Quality Standards and Testing Technology for Agricultural Products, Chinese Academy of Agricultural Science, Beijing 100081.
authors.forename: G L
authors.initials: GL
authors.lastname: Zhao
authors.affiliation: Institute of Quality Standards and Testing Technology for Agricultural Products, Chinese Academy of Agricultural Science, Beijing 100081.
authors.forename: P L
authors.initials: PL
authors.lastname: Wang
authors.affiliation: Institute of Quality Standards and Testing Technology for Agricultural Products, Chinese Academy of Agricultural Science, Beijing 100081 suxiaoou@caas.net.cn.
authors.forename: X O
authors.initials: XO
authors.lastname: Su
mesh_terms: D000318:Adrenergic beta-Agonists / Q000032:analysis* / Q000737:chemistry; D000821:Animal Feed / Q000032:analysis*; D002853:Chromatography, Liquid / Q000379:methods*; D057230:Limit of Detection; D016014:Linear Models; D011619:Psychotropic Drugs / Q000032:analysis* / Q000737:chemistry; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry / Q000379:methods*; D019155:Veterinary Drugs / Q000032:analysis* / Q000145:classification
chemical_list: D000318:Adrenergic beta-Agonists; D011619:Psychotropic Drugs; D019155:Veterinary Drugs