title: Development and characterization of LTA-appended chitosan nanoparticles for mucosal immunization against hepatitis B.
abstract: The present study was aimed at exploring the targeting potential of LTA-anchored chitosan nanoparticles (CH-NP) specifically to M cell following oral immunization. The lectinized CH-NP exhibited 7-29% coupling capacity depending upon the amount of glutaraldehyde added. Induction of the mucosal immunity was assessed by estimating secretory IgA level in the salivary, intestinal and vaginal secretions, and cytokine (IL-2 and IFN-γ) levels in the spleen homogenates. The results demonstrated that LTA-anchored CH-NP elicited strong humoral and cellular responses and hence could be a competent carrier-adjuvant delivery system for oral mucosal immunization against Hepatitis B.
authors.affiliation: Department of Pharmaceutical Sciences, Dr. H. S. Gour Central University , Sagar, MP , India.
authors.forename: Neeraj
authors.initials: N
authors.lastname: Mishra
authors.forename: Kapil
authors.initials: K
authors.lastname: Khatri
authors.forename: Madhu
authors.initials: M
authors.lastname: Gupta
authors.forename: Suresh P
authors.initials: SP
authors.lastname: Vyas
mesh_terms: D000818:Animals; D048271:Chitosan / Q000737:chemistry*; D005260:Female; D005976:Glutaral / Q000737:chemistry; D006509:Hepatitis B / Q000517:prevention & control*; D006514:Hepatitis B Surface Antigens / Q000276:immunology; D017325:Hepatitis B Vaccines / Q000737:chemistry* / Q000276:immunology*; D007114:Immunization / Q000379:methods*; D037102:Lectins / Q000737:chemistry*; D051379:Mice; D009092:Mucous Membrane / Q000276:immunology; D053758:Nanoparticles / Q000737:chemistry*; D010581:Peyer's Patches / Q000166:cytology
chemical_list: D006514:Hepatitis B Surface Antigens; D017325:Hepatitis B Vaccines; D037102:Lectins; C027112:fucose-binding lectin; D048271:Chitosan; D005976:Glutaral
keywords: LTA lectin; M cells; chitosan nanoparticles; hepatitis B; oral immunization