title: Assessment of oxidative metabolism.
abstract: Oxidative metabolism is one of the central physiological processes that regulate multiple functions in a cell including cell death and survival, proliferation, gene transcription, and protein modification. There are multitudes of techniques that are used to evaluate oxidative activity. Here, we summarize how to measure oxidative activity by flow cytometry. This versatile technique allows the evaluation of the level of oxidative activity within heterogeneous populations of cells and in cell culture. Flow cytometry is a quick method that yields highly reproducible results with small sample volumes. Therefore, it is an ideal technique for evaluating changes in oxidative activity in samples from mice.
authors.affiliation: Department of Biochemistry, Faculty of Medicine, University of Sherbrooke, Sherbrooke, QC, Canada.
authors.forename: Emilie
authors.initials: E
authors.lastname: Imbeault
authors.forename: Denis
authors.initials: D
authors.lastname: Gris
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D005434:Flow Cytometry / Q000379:methods*; D051379:Mice; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000235:genetics