title: Palmitoylation on conserved and nonconserved cysteines of murine IFITM1 regulates its stability and anti-influenza A virus activity.
abstract: The interferon-induced transmembrane proteins (IFITMs) restrict infection by numerous viruses, yet the importance and regulation of individual isoforms remains unclear. Here, we report that murine IFITM1 (mIFITM1) is palmitoylated on one nonconserved cysteine and three conserved cysteines that are required for anti-influenza A virus activity. Additionally, palmitoylation of mIFITM1 regulates protein stability by preventing proteasomal degradation, and modification of the nonconserved cysteine at the mIFITM1 C terminus supports an intramembrane topology with mechanistic implications.
authors.affiliation: Department of Microbial Infection and Immunity, Center for Microbial Interface Biology, The Ohio State University, Columbus, Ohio, USA.
authors.forename: Jocelyn C
authors.initials: JC
authors.lastname: Hach
authors.forename: Temet
authors.initials: T
authors.lastname: McMichael
authors.forename: Nicholas M
authors.initials: NM
authors.lastname: Chesarino
authors.forename: Jacob S
authors.initials: JS
authors.lastname: Yount
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000943:Antigens, Differentiation / Q000737:chemistry* / Q000235:genetics / Q000502:physiology*; D017124:Conserved Sequence; D003545:Cysteine / Q000737:chemistry / Q000235:genetics; D057809:HEK293 Cells; D006801:Humans; D009980:Influenza A virus / Q000472:pathogenicity* / Q000502:physiology; D054878:Lipoylation; D008565:Membrane Proteins / Q000737:chemistry / Q000235:genetics / Q000502:physiology; D051379:Mice; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D055550:Protein Stability; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D053586:Virus Internalization
chemical_list: D000943:Antigens, Differentiation; D008565:Membrane Proteins; D011994:Recombinant Proteins; C462181:fragilis protein, mouse; C062010:leu-13 antigen; D003545:Cysteine