title: Identification of two-step chemical mechanisms and determination of thermokinetic parameters using frequency responses to small temperature oscillations.
abstract: Increased focus on kinetic signatures in biology, coupled with the lack of simple tools for chemical dynamics characterization, lead us to develop an efficient method for mechanism identification. A small thermal modulation is used to reveal chemical dynamics, which makes the technique compatible with in cellulo imaging. Then, the detection of concentration oscillations in an appropriate frequency range followed by a judicious analytical treatment of the data is sufficient to determine the number of chemical characteristic times, the reaction mechanism, and the full set of associated rate constants and enthalpies of reaction. To illustrate the scope of the method, dimeric protein folding is chosen as a biologically relevant example of nonlinear mechanism with one or two characteristic times.
authors.affiliation: Université Pierre et Marie Curie-Paris 6, Laboratoire de Physique Théorique de la Matière Condensée, UMR 7600 LPTMC, 4 place Jussieu, case courrier 121, 75252 Paris cedex 05, France.
authors.forename: F
authors.initials: F
authors.lastname: Closa
authors.forename: C
authors.initials: C
authors.lastname: Gosse
authors.forename: L
authors.initials: L
authors.lastname: Jullien
authors.forename: A
authors.initials: A
authors.lastname: Lemarchand
mesh_terms: D007700:Kinetics; D017510:Protein Folding; D011506:Proteins / Q000737:chemistry*; D013696:Temperature
chemical_list: D011506:Proteins