title: In vitro bioactivity, cytocompatibility, and antibiotic release profile of gentamicin sulfate-loaded borate bioactive glass/chitosan composites.
abstract: Borate bioactive glass-based composites have been attracting interest recently as an osteoconductive carrier material for local antibiotic delivery. In the present study, composites composed of borate bioactive glass particles bonded with a chitosan matrix were prepared and evaluated in vitro as a carrier for gentamicin sulfate. The bioactivity, degradation, drug release profile, and compressive strength of the composite carrier system were studied as a function of immersion time in phosphate-buffered saline at 37 °C. The cytocompatibility of the gentamicin sulfate-loaded composite carrier was evaluated using assays of cell proliferation and alkaline phosphatase activity of osteogenic MC3T3-E1 cells. Sustained release of gentamicin sulfate occurred over ~28 days in PBS, while the bioactive glass converted continuously to hydroxyapatite. The compressive strength of the composite loaded with gentamicin sulfate decreased from the as-fabricated value of 24 ± 3 MPa to ~8 MPa after immersion for 14 days in PBS. Extracts of the soluble ionic products of the borate glass/chitosan composites enhanced the proliferation and alkaline phosphatase activity of MC3T3-E1 cells. These results indicate that the gentamicin sulfate-loaded composite composed of chitosan-bonded borate bioactive glass particles could be useful clinically as an osteoconductive carrier material for treating bone infection.
authors.affiliation: Institute of Bioengineering and Information Technology Materials, Tongji University, Shanghai, 200092, China.
authors.forename: Xu
authors.initials: X
authors.lastname: Cui
authors.forename: Yifei
authors.initials: Y
authors.lastname: Gu
authors.forename: Le
authors.initials: L
authors.lastname: Li
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
authors.forename: Zhongping
authors.initials: Z
authors.lastname: Xie
authors.forename: Shihua
authors.initials: S
authors.lastname: Luo
authors.forename: Nai
authors.initials: N
authors.lastname: Zhou
authors.forename: Wenhai
authors.initials: W
authors.lastname: Huang
authors.forename: Mohamed N
authors.initials: MN
authors.lastname: Rahaman
mesh_terms: D016475:3T3 Cells; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000737:chemistry; D001672:Biocompatible Materials / Q000627:therapeutic use; D001842:Bone and Bones / Q000473:pathology; D001881:Borates / Q000737:chemistry*; D002448:Cell Adhesion; D048271:Chitosan / Q000737:chemistry*; D019245:Compressive Strength; D016503:Drug Delivery Systems; D017886:Durapatite / Q000737:chemistry; D005839:Gentamicins / Q000008:administration & dosage*; D005898:Glass; D007477:Ions; D008422:Materials Testing; D051379:Mice; D008855:Microscopy, Electron, Scanning; D010012:Osteogenesis; D011312:Pressure; D017550:Spectroscopy, Fourier Transform Infrared; D013997:Time Factors
chemical_list: D000900:Anti-Bacterial Agents; D001672:Biocompatible Materials; D001881:Borates; D005839:Gentamicins; D007477:Ions; D048271:Chitosan; D017886:Durapatite; D000469:Alkaline Phosphatase