title: [Biodegradation of decabromodiphenyl ether by intracellular enzyme obtained from Pseudomonas aeruginosa].
abstract: The degradation characteristics of decabromodiphenyl ether (BDE-209) by crude enzyme from Pseudomonas aeruginosa were investigated. The results revealed that the degradation efficiency of the intracellular enzyme excreted from this bacterial strain reached 69.22% after incubation with 1 mg x L(-1) BDE-209 for 12 h. Temperature, pH, enzyme concentration and BDE-209 concentration all influenced the ability of crude enzyme to degrade BDE-209. When the BDE-209 concentration was 1 mg x L(-1), the optimal condition for enzymatic degradation was temperature 30 degrees C and pH 7.5, and the degradation rate increased with increasing enzyme concentration. The degradation process of BDE-209 by intracellular enzyme of the strain conformed to the first-order kinetic model. The highest reaction rate was achieved when the initial concentration of BDE-209 was 1 mg x L(-1) and the half-life of this substrate was 6.9 h. In addition, the biodegradation of BDE-209 can be well described by enzymatic reaction of high concentration substrate inhibition, with a maximum substrate utilization rate of 0.133 mg x (L x h)(-1), a Michaelis-Menten constant of 0.642 mg x L(-1), and an inhibitory constant of 1.558 mg x L(-1), respectively.
authors.affiliation: Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou 510640, China. shigylove@126.com
authors.forename: Guang-Yu
authors.initials: GY
authors.lastname: Shi
authors.forename: Hua
authors.initials: H
authors.lastname: Yin
authors.forename: Jin-Shao
authors.initials: JS
authors.lastname: Ye
authors.forename: Hui
authors.initials: H
authors.lastname: Peng
authors.forename: Na
authors.initials: N
authors.lastname: Zhang
authors.forename: Bao-Yan
authors.initials: BY
authors.lastname: He
mesh_terms: D001673:Biodegradation, Environmental; D004785:Environmental Pollutants / Q000302:isolation & purification* / Q000378:metabolism; D005411:Flame Retardants / Q000302:isolation & purification / Q000378:metabolism; D055768:Halogenated Diphenyl Ethers / Q000302:isolation & purification* / Q000378:metabolism; D007424:Intracellular Fluid / Q000201:enzymology*; D011550:Pseudomonas aeruginosa / Q000201:enzymology* / Q000378:metabolism
chemical_list: D004785:Environmental Pollutants; D005411:Flame Retardants; D055768:Halogenated Diphenyl Ethers; C010902:decabromobiphenyl ether