title: Development of an ultrasensitive immunoassay for detecting tartrazine.
abstract: We have developed an ultrasensitive indirect competitive enzyme-linked immunosorbent assay for the determination of tartrazine. Two carboxylated analogues of tartrazine with different spacer lengths, and one derivative from commercial tartrazine after a little chemical modification, were synthesized as haptens in order to produce antibodies specific to tartrazine. The effect of sulfonic acid groups on the hapten structure of tartrazine was also studied carefully for the first time. A most specific monoclonal antibody against tartrazine was created and exhibited an IC50 value of 0.105 ng/mL and a limit of detection of 0.014 ng/mL, with no cross-reactivity to other structurally-related pigments. The established immunoassay was applied to the determination of tartrazine in fortified samples of orange juice and in real positive samples of carbonated beverages.
authors.affiliation: State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Lihu Road 1800, Wuxi 214122, China. lzk669@126.com
authors.forename: Zhuokun
authors.initials: Z
authors.lastname: Li
authors.forename: Shanshan
authors.initials: S
authors.lastname: Song
authors.forename: Liguang
authors.initials: L
authors.lastname: Xu
authors.forename: Hua
authors.initials: H
authors.lastname: Kuang
authors.forename: Shidong
authors.initials: S
authors.lastname: Guo
authors.forename: Chuanlai
authors.initials: C
authors.lastname: Xu
mesh_terms: D001628:Beverages / Q000032:analysis*; D015374:Biosensing Techniques; D004797:Enzyme-Linked Immunosorbent Assay / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005504:Food Analysis / Q000295:instrumentation*; D005505:Food Coloring Agents / Q000032:analysis*; D008832:Microchemistry / Q000295:instrumentation*; D013645:Tartrazine / Q000032:analysis*
chemical_list: D005505:Food Coloring Agents; D013645:Tartrazine