title: Design and evaluation of capillary coupled with optical fiber light-emitting diode induced fluorescence detection for capillary electrophoresis.
abstract: A new detector, capillary coupled with optical fiber LED-induced fluorescence detector (CCOF-LED-IFD, using CCOF for short), is introduced for CE. The strategy of the present work was that the optical fiber and separation capillary were, in the parallel direction, fastened in a fixation capillary with larger inner diameter. By employing larger inner diameter, the fixation capillary allowed the large diameter of the optical fiber to be inserted into it. By transmitting an enhanced excitation light through the optical fiber, the detection sensitivity was improved. The advantages of the CCOF-CE system were validated by the detection of riboflavin, and the results were compared to those obtained by the in-capillary common optical fiber LED-induced fluorescence detector (IC-COF-LED-IFD, using COF for short). The LODs of CCOF-CE and COF-CE were 0.29 nM and 11.0 nM (S/N = 3), respectively. The intraday (n = 6) repeatability and interday (n = 6) reproducibility of migration time and corresponding peak area for both types of CE were all less than 1.10 and 3.30%, respectively. The accuracy of the proposed method was judged by employing standard addition method, and recoveries obtained were in the range of 98.0-102.4%. The results indicated that the sensitivity of the proposed system was largely improved, and that its reproducibility and accuracy were satisfactory. The proposed system was successfully applied to separate and determine riboflavin in real sample.
authors.affiliation: College of Chemistry, Sichuan University, Chengdu, P. R. China.
authors.forename: Hongyun
authors.initials: H
authors.lastname: Ji
authors.forename: Meng
authors.initials: M
authors.lastname: Li
authors.forename: Lihong
authors.initials: L
authors.lastname: Guo
authors.forename: Hongyan
authors.initials: H
authors.lastname: Yuan
authors.forename: Chunling
authors.initials: C
authors.lastname: Wang
authors.forename: Dan
authors.initials: D
authors.lastname: Xiao
mesh_terms: D019075:Electrophoresis, Capillary / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design; D057230:Limit of Detection; D016014:Linear Models; D008956:Models, Chemical; D055100:Optical Fibers; D015203:Reproducibility of Results; D012256:Riboflavin / Q000032:analysis; D013050:Spectrometry, Fluorescence / Q000295:instrumentation* / Q000379:methods
chemical_list: D012256:Riboflavin
keywords: Capillary coupled with optical fiber; Capillary electrophoresis; Common optical fiber; Fluorescence detection