title: Inflammatory response to Porphyromonas gingivalis partially requires interferon regulatory factor (IRF) 3.
abstract: Innate immune activation with expression of pro-inflammatory molecules such as TNF-α is a hallmark of the chronic inflammation associated with periodontal disease (PD). Porphyromonas gingivalis, a bacterium associated with PD, engages TLRs and activates MyD88-dependent and TIR-domain-containing adapter-inducing IFN-β (TRIF)-dependent signaling pathways. IFN regulatory factor (IRF) 3 is activated in a TRIF-dependent manner and participates in production of cytokines such as TNF-α; however, little is known regarding IRF3 and the host response to PD pathogens. We speculated that IRF3 participates in the host inflammatory response to P. gingivalis. Our results show that bone marrow macrophages (MØ) from WT mice respond to P. gingivalis with activation and nuclear translocation of IRF3. Compared with WT, MØ from IRF3(-/-), TRIF(-/-), and TLR4(-/-) mice responded with reduced levels of TNF-α on P. gingivalis challenge. In addition, full expression of IL-6 and RANTES by MØ to P. gingivalis was dependent on IRF3. Lastly, employing MØ from IRF3(-/-) and IRF7(-/-) mice we observed a significant role for IRF3 and a modest role for IRF7 in the P. gingivalis-elicited TNF-α response. These studies identify a role for IRF3 in the inflammatory response by MØ to the periodontal pathogen P. gingivalis.
authors.affiliation: Section of Infectious Diseases, Department of Medicine, Boston University School of Medicine, Boston, MA, USA.
authors.forename: Yazdani B
authors.initials: YB
authors.lastname: Shaik-Dasthagirisaheb
authors.forename: Nasi
authors.initials: N
authors.lastname: Huang
authors.forename: Frank C
authors.initials: FC
authors.lastname: Gibson
mesh_terms: D033942:Adaptor Proteins, Vesicular Transport / Q000235:genetics / Q000502:physiology; D000818:Animals; D001442:Bacteroides Infections / Q000276:immunology / Q000378:metabolism / Q000503:physiopathology*; D002467:Cell Nucleus / Q000378:metabolism; D018946:Chemokine CCL5 / Q000096:biosynthesis; D018925:Chemokines / Q000096:biosynthesis; D016207:Cytokines / Q000096:biosynthesis; D007113:Immunity, Innate / Q000502:physiology*; D007249:Inflammation / Q000276:immunology / Q000378:metabolism / Q000503:physiopathology*; D050838:Interferon Regulatory Factor-3 / Q000235:genetics / Q000502:physiology*; D050839:Interferon Regulatory Factor-7 / Q000378:metabolism; D015850:Interleukin-6 / Q000096:biosynthesis; D008264:Macrophages / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D016966:Porphyromonas gingivalis / Q000254:growth & development / Q000276:immunology*; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
chemical_list: D033942:Adaptor Proteins, Vesicular Transport; D018946:Chemokine CCL5; D018925:Chemokines; D016207:Cytokines; D050838:Interferon Regulatory Factor-3; D050839:Interferon Regulatory Factor-7; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha; C476286:Ticam1 protein, mouse
keywords: IRF3; Porphyromonas gingivalis; chemokine; cytokine; macrophage