title: [Roles of protease-activated receptor-1 in thrombin-induced brain injury and neurogenesis in rats].
abstract: OBJECTIVE
  To investigate the roles of protease-activated receptors (PARs) in thrombin-induced brain injury and neurogenesis in rats.
  
  
  METHODS
  Ninety male SD rats were randomly assigned to receive intra-hippocampus injection of NS, thrombin or specific agonists of 3 protease-activated receptors (PAR-1, PAR-3 and PAR-4), respectively. At 1,3 and 7 d after injection, the area of the hippocampus was determined with HE staining, the density and morphology of astrocyte were detected with GFAP staining, degenerated neurons were detected with Fluoro-Jade C staining, and the neurogenesis was examined with DCX staining.
  
  
  RESULTS
  Compared to NS injection, the area of the hippocampus significantly increased at 1-3 d and decreased at 7 d after the injection of thrombin and PAR-1 agonist (P<0.05). In addition, injection of thrombin and PAR-1 agonist significantly increased the density of astrocyte and Fluoro-Jade C positive cells at 1-7 d after injection (P<0.05), and significantly increased the density of DCX positive cells at 3-7 d after injection(P<0.05). The injection of PAR-3 agonist and PAR-4 agonist had no affect on the area of the hippocampus, the density of astrocyte, Fluoro-Jade C positive cells and DCX positive cells.
  
  
  CONCLUSIONS
  The activation of protease-activated receptor-1 may be related to the thrombin-induced brain injury and neurogenesis in rat hippocampus.
authors.affiliation: The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310009, China.
authors.forename: Feifei
authors.initials: F
authors.lastname: Liu
authors.forename: Fuyi
authors.initials: F
authors.lastname: Liu
authors.forename: Lin
authors.initials: L
authors.lastname: Wang
authors.forename: Hua
authors.initials: H
authors.lastname: Hu
mesh_terms: D000818:Animals; D001930:Brain Injuries / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology*; D000089662:Doublecortin Protein; D006624:Hippocampus / Q000473:pathology; D008297:Male; D055495:Neurogenesis / Q000187:drug effects*; D051381:Rats; D017207:Rats, Sprague-Dawley; D044463:Receptor, PAR-1 / Q000819:agonists* / Q000502:physiology*; D018179:Receptors, Thrombin / Q000819:agonists; D013917:Thrombin / Q000633:toxicity*
chemical_list: C000714848:Dcx protein, rat; D000089662:Doublecortin Protein; D044463:Receptor, PAR-1; D018179:Receptors, Thrombin; C105295:protease-activated receptor 3; D013917:Thrombin; C112908:protease-activated receptor 4