title: A microfluidic system to study the cytotoxic effect of drugs: the combined effect of celecoxib and 5-fluorouracil on normal and cancer cells.
abstract: We have investigated the response of normal and cancer cells to exposure a combination of celecoxib (Celbx) and 5-fluorouracil (5-FU) using a lab-on-a-chip microfluidic device. Specifically, we have tested the cytotoxic effect of Celbx on normal mouse embryo cells (Balb/c 3T3) and human lung carcinoma cells (A549). The single drugs or their combinations were adjusted to five different concentrations using a concentration gradient generator (CGG) in a single step. The results suggest that Celbx can enhanced the anticancer activity of 5-FU by stronger inhibition of cancer cell growth. We also show that the A549 cancer cells are more sensitive to Celbx than the Balb/c 3T3 normal cells. The results obtained with the microfluidic system were compared to those obtained with a macroscale in vitro cell culture method. In our opinion, the microfluidic system represents a unique approach for an evaluation of cellular response to multidrug exposure that also is more simple than respective microwell plate assays. Figureᅟ
authors.affiliation: Department of Microbioanalytics, Institute of Biotechnology, Warsaw University of Technology, 00-664 Warsaw, Poland.
authors.forename: Elzbieta
authors.initials: E
authors.lastname: Jastrzebska
authors.forename: Sylwia
authors.initials: S
authors.lastname: Flis
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Rakowska
authors.forename: Michal
authors.initials: M
authors.lastname: Chudy
authors.forename: Zenon
authors.initials: Z
authors.lastname: Jastrzebski
authors.forename: Artur
authors.initials: A
authors.lastname: Dybko
authors.forename: Zbigniew
authors.initials: Z
authors.lastname: Brzozka
keywords: 5-fluorouracil; Celecoxib; Chemotherapy resistance; Lab-on-a-chip; Microfluidic gradient system; Multidrug tests