title: A novel chromogenic screening medium for isolation of enterohemorrhagic Escherichia coli.
abstract: EHEC-chrom, a novel chromogenic screening agar medium for enterohemorrhagic Escherichia coli (EHEC) , was developed. A total of 52 EHEC strains, which were studied for the inclusivity study, grew and formed blue-green colored colonies on EHEC-chrom. When 43 gram-negative bacteria other than EHEC were inoculated for the exclusivity study, 10 strains grew and formed colorless colonies. A total of 28 gram-positive bacteria failed to grow and 1 yeast strain grew as colorless colonies. EHEC-chrom was compared with CHROMagar™ STEC, XM-EHEC agar and CT-MacConkey base agar with a specific sugar (CT-SorbitolMAC, CT-rhamnoseMAC and CT-sorboseMAC) as commercially available selective agar using 100 food samples artificially contaminated with low levels (<10 logCFU/25g) of EHEC. Numbers of samples from which EHEC was recovered by using EHEC-chrom, CHROMagar™ STEC, XM-EHEC agar and CT-MacConkey base agar with specific sugar were 62, 58, 59 and 60, respectively. Our results suggested EHEC-chrom was a useful alternative for EHEC screening in food.
authors.affiliation: Microbiology Research Division, Kohjin Bio Co., Ltd., 5-1-3 Chiyoda, Sakado, Saitama 350-0214, Japan. teramura@kohjin-bio.co.jp
authors.forename: Hajime
authors.initials: H
authors.lastname: Teramura
authors.forename: Jun-Ichiro
authors.initials: J
authors.lastname: Sekiguchi
authors.forename: Kohaku
authors.initials: K
authors.lastname: Inoue
mesh_terms: D002863:Chromogenic Compounds / Q000378:metabolism; D015169:Colony Count, Microbial / Q000295:instrumentation / Q000379:methods*; D003470:Culture Media / Q000737:chemistry / Q000378:metabolism*; D054324:Enterohemorrhagic Escherichia coli / Q000254:growth & development / Q000302:isolation & purification* / Q000378:metabolism; D004927:Escherichia coli Infections / Q000382:microbiology*; D006801:Humans
chemical_list: D002863:Chromogenic Compounds; D003470:Culture Media