title: Engineered nanostructured β-sheet peptides protect membrane proteins.
abstract: We designed β-strand peptides that stabilize integral membrane proteins (IMPs). β-strand peptides self-assemble in solution as filaments and become restructured upon association with IMPs; resulting IMP-β-strand peptide complexes resisted aggregation when diluted in detergent-free buffer and were visible as stable, single particles with low detergent background in electron micrographs. β-strand peptides enabled clear visualization of flexible conformations in the highly dynamic ATP-binding cassette (ABC) transporter MsbA.
authors.affiliation: Department of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, California, USA.
authors.forename: Houchao
authors.initials: H
authors.lastname: Tao
authors.forename: Sung Chang
authors.initials: SC
authors.lastname: Lee
authors.forename: Arne
authors.initials: A
authors.lastname: Moeller
authors.forename: Rituparna Sinha
authors.initials: RS
authors.lastname: Roy
authors.forename: Fai Yiu
authors.initials: FY
authors.lastname: Siu
authors.forename: Jörg
authors.initials: J
authors.lastname: Zimmermann
authors.forename: Raymond C
authors.initials: RC
authors.lastname: Stevens
authors.forename: Clinton S
authors.initials: CS
authors.lastname: Potter
authors.forename: Bridget
authors.initials: B
authors.lastname: Carragher
authors.forename: Qinghai
authors.initials: Q
authors.lastname: Zhang
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000737:chemistry; D001426:Bacterial Proteins / Q000737:chemistry; D002942:Circular Dichroism; D008565:Membrane Proteins / Q000138:chemical synthesis / Q000737:chemistry*; D046529:Microscopy, Electron, Transmission; D049329:Nanostructures / Q000737:chemistry*; D010455:Peptides / Q000138:chemical synthesis* / Q000737:chemistry*; D017433:Protein Structure, Secondary; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D018528:ATP-Binding Cassette Transporters; D001426:Bacterial Proteins; D008565:Membrane Proteins; C079726:MsbA protein, Bacteria; D010455:Peptides