title: Values of MMP-2 and MMP-9 in tumor tissue of basal-like breast cancer patients.
abstract: Gelatinase A (MMP-2) and gelatinase B (MMP-9) are proteolytic enzymes involved in process of tumor invasion, and they are considered as possible tumor markers in breast cancer patients. In this study, we measured activity of latent and active form of MMP-2 and MMP-9 in tumor and adjacent tissue of 60 breast cancer patients by SDS-PAGE zymography. The activity of both form of gelatinases significantly increased with each advancing clinical stage of disease. ProMMP-9 and aMMP-9 activity in tumor tissue shows a positive association with tumor size. Patients with lymph node involvement have higher proMMP-2, aMMP-2 and aMMP-9 activity than node negative patients. Steroid receptor-negative tumors had enhanced aMMP-2 and aMMP-9 activity. Patients with basal-like cancers had higher proMMP-2 tumor activity and aMMP-2 adjacent tissue activity compared to patients with luminal A tumors. Patients with negative hormone receptors are associated with increased activity of both form of gelatinases in adjacent tissue. Reported increased activity of MMP-2 in tumor and adjacent tissue of basal-like tumors implicates that MMP-2 might have a role in aggressive biology of basal-like cancers. Additional investigations regarding molecular pathways in adjacent tissue could give better insight into aggressive nature of basal-like carcinomas.
authors.affiliation: Department of Experimental Oncology, Institute of Oncology and Radiology of Serbia, Pasterova 14, 11 000, Belgrade, Serbia, stankovics@ncrc.ac.rs.
authors.forename: Sandra
authors.initials: S
authors.lastname: Radenkovic
authors.forename: Gordana
authors.initials: G
authors.lastname: Konjevic
authors.forename: Vladimir
authors.initials: V
authors.lastname: Jurisic
authors.forename: Katarina
authors.initials: K
authors.lastname: Karadzic
authors.forename: Marina
authors.initials: M
authors.lastname: Nikitovic
authors.forename: Kristina
authors.initials: K
authors.lastname: Gopcevic
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000378:metabolism*; D001943:Breast Neoplasms / Q000201:enzymology* / Q000473:pathology; D004792:Enzyme Precursors / Q000378:metabolism*; D005260:Female; D006801:Humans; D008207:Lymphatic Metastasis; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism*; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism*; D008875:Middle Aged; D009367:Neoplasm Staging
chemical_list: D014408:Biomarkers, Tumor; D004792:Enzyme Precursors; C120487:pro-matrix metalloproteinase 9; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9