title: Phenotypic changes in Cyprinus carpiovar var. Jian introduced by sperm-mediated transgenesis of rearranged homologous DNA fragments.
abstract: Common carp, specifically the Jian variety (Cyprinus carpiovar var. Jian), is an important Chinese and global aquatic stock for commercial foodstuff. Homologous recombination of carp gene sequences has been widely used in population genetics to broadly screen for beneficial phenotypical variations, thus optimizing artificially engineered carp stocks with Jian variety and native stock varieties. Random rearrangement of homologous DNA fragments from parent specimens of C. carpiovar var. Jian were attained by digestion of genomic DNA with MspI followed by religation and redigestion with EcoR I to specifically rearrange homologous DNA fragments of myostatin and microsatellite genes. Based on known characteristics of myostatin gene function, growth pattern changes in resultant carp mutant varieties was expected. DNA fragments were introduced into metaphase-II oocytes, resulting in one to several dozen insertions of homologous fragments into the host genome by sperm-mediated transgenesis. Introduction of rearranged homologous DNA fragments often resulted in phenotypic changes in C. carpiovar var. Jian, including significant phenotypic changes linked to growth rate at 4 months.
authors.affiliation: Chinese Academy of Fishery Sciences, Wuxi, China. caozm@ffrc.cn
authors.forename: Zheming
authors.initials: Z
authors.lastname: Cao
authors.forename: Weidong
authors.initials: W
authors.lastname: Ding
authors.forename: Hongtao
authors.initials: H
authors.lastname: Ren
mesh_terms: D000818:Animals; D001947:Breeding; D002347:Carps / Q000235:genetics*; D004247:DNA / Q000737:chemistry / Q000235:genetics*; D018014:Gene Transfer Techniques; D059765:Homologous Recombination; D008297:Male; D009154:Mutation; D055435:Myostatin / Q000235:genetics; D010641:Phenotype; D013094:Spermatozoa / Q000378:metabolism*
chemical_list: D055435:Myostatin; D004247:DNA