title: Binding of curcumin with bovine serum albumin in the presence of ι-carrageenan and implications on the stability and antioxidant activity of curcumin.
abstract: This work studied the influences of formation of BSA/ι-carrageenan complexes on the binding, stability, and antioxidant activity of curcumin. In the presence of BSA and ι-carrageenan, curcumin gives higher intensities of absorption and fluorescence than free curcumin and curcumin only combined with BSA. The added ι-carrageenan is observed to promote curcumin for quenching the instrinsic fluorescence of BSA. These results are explained in terms of the formation of BSA/ι-carrageenan complexes, which help to stabilize the folded structure of BSA for providing curcumin with a more hydrophobic microenvironment. The small difference in anisotropy values of curcumin with BSA alone and of BSA/ι-carrageenan complexes suggests that ι-carrageenan acts as outer stretch conformation in BSA/ι-carrageenan complexes but does not directly disturb the hydrophobic pockets inside BSA, where curcumin is hydrophobically located. The determined values of the binding constant are higher for curcumin with BSA/ι-carrageenan complexes than with BSA alone. Moreover, BSA/ι-carrageenan complexes are found to be superior to single BSA for enhancing the stability and DPPH radical-scavenging ability of curcumin.
authors.affiliation: School of Chemistry and Molecular Engineering, East China University of Science and Technology , Shanghai 200237, China.
authors.forename: Mingling
authors.initials: M
authors.lastname: Yang
authors.forename: Yue
authors.initials: Y
authors.lastname: Wu
authors.forename: Jinbing
authors.initials: J
authors.lastname: Li
authors.forename: Haibo
authors.initials: H
authors.lastname: Zhou
authors.forename: Xiaoyong
authors.initials: X
authors.lastname: Wang
mesh_terms: D002351:Carrageenan / Q000737:chemistry*; D003474:Curcumin / Q000737:chemistry* / Q000378:metabolism* / Q000494:pharmacology; D004355:Drug Stability; D005453:Fluorescence; D005454:Fluorescence Polarization; D006863:Hydrogen-Ion Concentration; D057927:Hydrophobic and Hydrophilic Interactions; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000378:metabolism*
chemical_list: D012710:Serum Albumin, Bovine; D002351:Carrageenan; D003474:Curcumin