title: Effect of aqueous extract and fractions of Fagonia arabica on in vitro anticoagulant activity.
abstract: Fagonia arabica (FA) is a deobstruent and blood purifier, which possesses thrombolytic and antioxidant activities. In this study, the anticoagulant effects of FA and its derived fractions were evaluated. Plasma recalcification was performed with multisolvent extracts of FA and then with extracts prepared successively with increasing polarity of the solvents. Aqueous extract was the most potent anticoagulant extract, which was fractionated by thin-layer chromatography and column chromatography. Five fractions collected were checked for their anticoagulation effect. The most potent fraction was screened for phytoconstituents. Aqueous extract of FA is the most active anticoagulant (31 minutes). Results were statistically significant when compared to heparin (38 minutes) and saline (4.04 minutes; P > .001). The Fifth fraction (FA5), the most potent fraction (27 minutes), was found positive for flavonoids, saponin, tannin, triterpenoids, carbohydrates, reducing sugar, and monosaccharides. Aqueous FA and fraction FA5 were most active in in vitro anticoagulation, and any of the phytochemicals identified could be considered the active component.
authors.affiliation: Biochemistry Research Laboratory, Central India Institute of Medical Sciences, Nagpur, Maharashtra, India.
authors.forename: Sweta R
authors.initials: SR
authors.lastname: Chourasia
authors.affiliation: Biochemistry Research Laboratory, Central India Institute of Medical Sciences, Nagpur, Maharashtra, India raj_ciims@rediffmail.com.
authors.forename: Rajpal Singh
authors.initials: RS
authors.lastname: Kashyap
authors.affiliation: Biochemistry Research Laboratory, Central India Institute of Medical Sciences, Nagpur, Maharashtra, India.
authors.forename: Jayant Y
authors.initials: JY
authors.lastname: Deopujari
authors.affiliation: Environmental Genomics Unit, National Environmental and Engineering Research Institute, Nagpur, Maharashtra, India.
authors.forename: Hemant J
authors.initials: HJ
authors.lastname: Purohit
authors.affiliation: Biochemistry Research Laboratory, Central India Institute of Medical Sciences, Nagpur, Maharashtra, India.
authors.forename: Girdhar M
authors.initials: GM
authors.lastname: Taori
authors.affiliation: Biochemistry Research Laboratory, Central India Institute of Medical Sciences, Nagpur, Maharashtra, India.
authors.forename: Hatim F
authors.initials: HF
authors.lastname: Daginawala
mesh_terms: D000925:Anticoagulants / Q000494:pharmacology*; D006801:Humans; D066298:In Vitro Techniques; D010936:Plant Extracts / Q000494:pharmacology*; D028461:Zygophyllaceae / Q000737:chemistry*
chemical_list: D000925:Anticoagulants; D010936:Plant Extracts
keywords: Fagonia arabica; anticoagulant activity; phytochemicals; thrombosis