title: Fermentation of biodiesel-derived glycerol by Bacillus amyloliquefaciens: effects of co-substrates on 2,3-butanediol production.
abstract: Cultivation in glycerol instead of sugars inhibits 2,3-butanediol (2,3-BD) production by Bacillus amyloliquefaciens. In this study, we report that B. amyloliquefaciens readily produces 2,3-BD from biodiesel-derived glycerol in the presence of beet molasses as a co-substrate. Unexpectedly, the molasses stimulated 2,3-BD production and simultaneously reduced the duration of fermentation. Productivity of 2,3-BD was enhanced at the start of fermentation, and yields increased under continuous molasses supply. Subsequently, 2,3-BD production in molasses-supplemented fed-batch culture was observed. Prior to inoculation of fed-batch fermentation culture, 15 g/l of molasses was added to the bioreactor. After 6 h of incubation, the bioreactor was fed with a solution containing 80 % glycerol and 15 % molasses. The 2,3-BD concentration, yield, and productivity significantly improved, reaching 83.3 g/l, 0.42 g/g, and 0.87 g/l·h, respectively. To our knowledge, these results are the highest report for 2,3-BD fermentation from biodiesel-derived glycerol.
authors.affiliation: Key Laboratory of Industrial Biotechnology of Ministry of Education, School of Biotechnology, Jiangnan University, 1800 Lihu Avenue, Wuxi, Jiangsu, 214122, People’s Republic of China.
authors.forename: Tao-Wei
authors.initials: TW
authors.lastname: Yang
authors.forename: Zhi-Ming
authors.initials: ZM
authors.lastname: Rao
authors.forename: Xian
authors.initials: X
authors.lastname: Zhang
authors.forename: Mei-Juan
authors.initials: MJ
authors.lastname: Xu
authors.forename: Zheng-Hong
authors.initials: ZH
authors.lastname: Xu
authors.forename: Shang-Tian
authors.initials: ST
authors.lastname: Yang
mesh_terms: D001407:Bacillus / Q000378:metabolism*; D056804:Biofuels / Q000032:analysis*; D019149:Bioreactors / Q000382:microbiology; D002072:Butylene Glycols / Q000378:metabolism*; D003470:Culture Media / Q000378:metabolism; D005285:Fermentation; D005990:Glycerol / Q000378:metabolism*; D008965:Molasses / Q000032:analysis
chemical_list: D056804:Biofuels; D002072:Butylene Glycols; D003470:Culture Media; C026978:2,3-butylene glycol; D005990:Glycerol