title: Identification of stem cells after transplantation.
abstract: The ability to identify the donor stem cells following transplantation into injured hearts is critical. This is particularly important in evaluating stem cell survival and lineage differentiation into mature cardiovascular cells. Several approaches have been employed for tracking the donor stem cells, including fluorescent dyes and fluorescent protein gene transfer. Here, we will induce a protocol using lentivirus-mediated green fluorescent protein (GFP) to monitor the fate of donor stem cells following transplantation.
authors.affiliation: Department of Internal Medicine, Division of Cardiovascular Disease, College of Medicine, University of Cincinnati, Cincinnati, OH, USA.
authors.forename: Yingjie
authors.initials: Y
authors.lastname: Wang
authors.forename: Lan
authors.initials: L
authors.lastname: Zhang
authors.forename: Yaohua
authors.initials: Y
authors.lastname: Pan
authors.forename: Lijuan
authors.initials: L
authors.lastname: Chen
authors.forename: Neal
authors.initials: N
authors.lastname: Weintraub
authors.forename: Yaoliang
authors.initials: Y
authors.lastname: Tang
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002470:Cell Survival; D002478:Cells, Cultured; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism*; D016086:Lentivirus / Q000235:genetics*; D051379:Mice; D009203:Myocardial Infarction / Q000378:metabolism* / Q000473:pathology; D033581:Stem Cell Transplantation / Q000379:methods*; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism; D014019:Tissue Donors
chemical_list: D049452:Green Fluorescent Proteins