title: The Solanum chacoense ovary receptor kinase 11 (ScORK11) undergoes tissue-dependent transcriptional, translational and post-translational regulation.
abstract: Using a subtraction screen to isolate weakly expressed transcripts from ovule and ovary libraries, we uncovered 30 receptor-like kinases that were predominantly expressed in ovary and fruit tissues following fertilization [1]. Here we describe the analysis of Solanum chacoense ovule receptor kinase 11 (ScORK11), a member of the large LRR III receptor kinase subfamily that localizes to the plasma membrane. In situ analyses demonstrated that ScORK11 gene expression was mainly restricted to the ovule integument, the embryo sac and the pericarp of the fruit. Tight regulation of ScORK11 expression at the mRNA level was also accompanied by both translational and post-translational regulation of protein levels.
authors.affiliation: Département de chimie et physique, Université du Québec à Trois-Rivières, 3351 boulevard des Forges, Trois-Rivières, QC G9A 5H7, Canada. hugo.germain@uqtr.ca
authors.forename: Hugo
authors.initials: H
authors.lastname: Germain
authors.forename: Madoka
authors.initials: M
authors.lastname: Gray-Mitsumune
authors.forename: Josée
authors.initials: J
authors.lastname: Houde
authors.forename: Rachid
authors.initials: R
authors.lastname: Benhamman
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Sawasaki
authors.forename: Yaeta
authors.initials: Y
authors.lastname: Endo
authors.forename: Daniel P
authors.initials: DP
authors.lastname: Matton
mesh_terms: D002462:Cell Membrane; D035264:Flowers / Q000378:metabolism*; D005638:Fruit / Q000378:metabolism*; D015870:Gene Expression; D015971:Gene Expression Regulation, Enzymologic; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D005810:Multigene Family; D056771:Ovule / Q000378:metabolism; D010770:Phosphotransferases / Q000235:genetics* / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D014176:Protein Biosynthesis; D011499:Protein Processing, Post-Translational; D012333:RNA, Messenger / Q000378:metabolism; D012639:Seeds / Q000378:metabolism; D032322:Solanum / Q000235:genetics* / Q000378:metabolism
chemical_list: D010940:Plant Proteins; D012333:RNA, Messenger; D010770:Phosphotransferases
keywords: Fertilization; Ovule; Post-translational regulation; Receptor kinase; Solanum chacoense