title: Molecular characterization of five steroid receptors from pengze crucian carp and their expression profiles of juveniles in response to 17α-ethinylestradiol and 17α-methyltestosterone.
abstract: Pengze crucian carp (Carassius auratus var. pengze, Pcc), a triploid gynogenetic fish, was used in this study to investigate the cross-talk between EDCs and steroid receptors. The full-length cDNAs of five steroid receptors (esr1, er alpha2, esr2a, esr2b, ar) and partial cDNA of vtg B were isolated. The tissue distributions of these genes were analyzed in adult fish by qRT-PCR. Then the expression profiles of five steroid receptors (esrs and ar) and vtg B were detected in the juveniles exposed to 17α-ethinylestradiol (EE2, 0.1, 1 and 10ng/L) and 17α-methyltestosterone (MT, 50μg/L) for 4weeks. The results demonstrated that esrs, ar, and vtg B were predominantly expressed in liver of adult fish. However, among these detected genes, esr1 and er alpha2 mRNAs are sensitive biomarkers in response to EE2 at 0.1, 1, and 10ng/L for 1 and 2weeks compared to esr2a, esr2b, ar, and vtg B in the juveniles of mono-female gynogenetic fish. Totally, the subtypes of esrs show biphasic responses to EE2 exposures for 4weeks, and most of the EE2 exposures at 0.1, 1, and 10ng/L for 1, 2, 3 and 4weeks did not induce the mRNA expressions of vtg B. However, 1-, 2-, and 4-week 50μg/L MT all significantly stimulated vtg B transcripts. Further investigations are needed to elucidate the mechanism underlying the insensitivity or down-regulation of vtg B mRNA in response to EE2 in juvenile Pcc.
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling, Shaanxi 712100, China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Zheng
authors.forename: Lihong
authors.initials: L
authors.lastname: Wang
authors.forename: Meng
authors.initials: M
authors.lastname: Li
authors.forename: Hongwei
authors.initials: H
authors.lastname: Liang
authors.forename: Fang
authors.initials: F
authors.lastname: Qin
authors.forename: Shaozhen
authors.initials: S
authors.lastname: Liu
authors.forename: Houpeng
authors.initials: H
authors.lastname: Wang
authors.forename: Tingting
authors.initials: T
authors.lastname: Wu
authors.forename: Yingying
authors.initials: Y
authors.lastname: Zhang
authors.forename: Zaizhao
authors.initials: Z
authors.lastname: Wang
mesh_terms: D000818:Animals; D002347:Carps / Q000378:metabolism*; D004997:Ethinyl Estradiol / Q000494:pharmacology*; D005260:Female; D008297:Male; D008777:Methyltestosterone / Q000494:pharmacology*; D011987:Receptors, Steroid / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D011987:Receptors, Steroid; D004997:Ethinyl Estradiol; D008777:Methyltestosterone
keywords: 17α-Ethinylestradiol; 17α-Methyltestosterone; 17α-ethinylestradiol; 17α-methyltestosterone; AR; Androgen receptor; CYP; DMSO; EDCs; EE2; ERE; ESR; Estrogen receptor; Juvenile pengze crucian carp; LRH-A; MAPK; MT; PKA; PKC; Vitellogenin; Vtg; androgen receptor; cytochrome P450; dimethyl sulfoxide; endocrine disrupting chemicals; estrogen receptor; estrogen-responsive element; hCG; human chorionic gonadotropin; luteinizing hormone releasing hormone analog; mitogen activated protein kinase; protein kinase A; protein kinase C; vitellogenin