title: Synthesis of D-mannose capped silicon nanoparticles and their interactions with MCF-7 human breast cancerous cells.
abstract: Silicon nanoparticles (SiNPs) hold prominent interest in various aspects of biomedical applications. For this purpose, surface functionalization of the NPs is essential to stabilize them, target them to specific disease area, and allow them to selectively bind to the cells or the bio-molecules present on the surface of the cells. However, no such functionalization has been explored with Si nanoparticles. Carbohydrates play a critical role in cell recognition. Here, we report the first synthesis of silicon nanoparticles functionalized with carbohydrates. In this study, stable and brightly luminescent d-Mannose (Man) capped SiNPs have been synthesized from amine terminated SiNPs and d-mannopyranoside acid. The surface functionalization is confirmed by Fourier transform infrared spectroscopy (FTIR), nuclear magnetic resonance spectroscopy (NMR), and energy dispersive X-ray spectroscopy (EDX) studies. The mean diameter of the crystal core is 5.5 nm, as measured by transmission electron microscopy (TEM), while the hydrodynamic diameter obtained by dynamic light scattering (DLS) is 16 nm. The quantum yield (QY) of photoluminescence emission is found to be 11.5%, and the nanoparticles exhibit an exceptional stability over two weeks. The Man-capped SiNPs may prove to be valuable tools for further investigating glycobiological, biomedical, and material science fields. Experiments are carried out using Concanavalin A (ConA) as a target protein in order to prove the hypothesis. When Man functionalized SiNPs are treated with ConA, cross-linked aggregates are formed, as shown in TEM images as well as monitored by photoluminescence spectroscopy (PL). Man functionalized SiNPs can target cancerous cells. Visualization imaging of SiNPs in MCF-7 human breast cancer cells shows the fluorescence is distributed throughout the cytoplasm of these cells.
authors.affiliation: School of Chemistry, University of East Anglia, Norwich NR4 7TJ, United Kingdom.
authors.forename: Jayshree H
authors.initials: JH
authors.lastname: Ahire
authors.forename: Isabelle
authors.initials: I
authors.lastname: Chambrier
authors.forename: Anja
authors.initials: A
authors.lastname: Mueller
authors.forename: Yongping
authors.initials: Y
authors.lastname: Bao
authors.forename: Yimin
authors.initials: Y
authors.lastname: Chao
mesh_terms: D001943:Breast Neoplasms / Q000378:metabolism*; D045744:Cell Line, Tumor; D003208:Concanavalin A / Q000737:chemistry*; D003593:Cytoplasm / Q000378:metabolism; D005260:Female; D006801:Humans; D008027:Light; D061986:MCF-7 Cells; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008358:Mannose / Q000737:chemistry*; D008422:Materials Testing; D046529:Microscopy, Electron, Transmission / Q000379:methods; D053758:Nanoparticles / Q000737:chemistry*; D036103:Nanotechnology / Q000379:methods*; D012542:Scattering, Radiation; D012825:Silicon / Q000737:chemistry*; D013052:Spectrometry, X-Ray Emission; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods; D013499:Surface Properties
chemical_list: D003208:Concanavalin A; D008358:Mannose; D012825:Silicon