title: Correlation between magnifying narrow-band imaging endoscopy results and organoid differentiation indicated by cancer cell differentiation and its distribution in depressed- type early gastric carcinoma.
abstract: BACKGROUND
  A close association between patterns identified by magnifying narrow-band imaging (M-NBI) and histological type has been described. M-NBI patterns were also recently reported to be related to the mucin phenotype; however, detials remain unclear.
  
  
  METHODS
  We investigated the cellular differentiation of gastric cancer lesions, along with their mucosal distribution observed by M-NBI. Ninety-seven depressed-type early gastric cancer lesions (74 differentiated and 23 undifferentiated adenocarcinomas) were visualized by M-NBI. Findings were divided into 4 patterns based on abnormal microvascular architecture: a chain loop pattern (CLP), a fine network pattern (FNP), a corkscrew pattern (CSP), and an unclassified pattern. Mucin phenotypes were judged as gastric (G-type), intestinal (I-type), mixed gastric and intestinal (M-type), and null (N-type) based on 4 markers (MAC5AC, MUC6, MUC2, and CD10). The relationship of each pattern of microvascular architecture with organoid differentiation indicated by cancer cell differentiation and its distribution in each histological type of early gastric cancer was investigated.
  
  
  RESULTS
  All CLP and FNP lesions were differentiated. The cancer cell distribution showed organoid differentiation in 84.2% (16/19) and 61.1% (22/36) of the two types of lesions, respectively, and there was a significant difference from the unclassified pattern with organoid differentiation (p<0.001). Almost all (94.7%; 18/19) CSP lesions were undifferentiated, and organoid differentiation was observed in 72.2% (13/18). There was a significant difference from the unclassified pattern with organoid differentiation (p<0.05).
  
  
  CONCLUSIONS
  Cellular differentiation and distribution are associated with microvascular architecture observed by M-NBI.
authors.affiliation: Department of Gastroenterology and Hepatology, Nagoya University Graduate School of Medicine, Nagoya, Japan.
authors.forename: Hidezumi
authors.initials: H
authors.lastname: Tatematsu
authors.forename: Ryoji
authors.initials: R
authors.lastname: Miyahara
authors.forename: Yoshie
authors.initials: Y
authors.lastname: Shimoyama
authors.forename: Kohei
authors.initials: K
authors.lastname: Funasaka
authors.forename: Eizaburou
authors.initials: E
authors.lastname: Ohno
authors.forename: Masanao
authors.initials: M
authors.lastname: Nakamura
authors.forename: Hiroki
authors.initials: H
authors.lastname: Kawashima
authors.forename: Akihiro
authors.initials: A
authors.lastname: Itoh
authors.forename: Naoki
authors.initials: N
authors.lastname: Ohmiya
authors.forename: Yoshiki
authors.initials: Y
authors.lastname: Hirooka
authors.forename: Osamu
authors.initials: O
authors.lastname: Watanabe
authors.forename: Osamu
authors.initials: O
authors.lastname: Maeda
authors.forename: Takafumi
authors.initials: T
authors.lastname: Ando
authors.forename: Hidemi
authors.initials: H
authors.lastname: Goto
mesh_terms: D000368:Aged; D002454:Cell Differentiation; D004724:Endoscopy; D006801:Humans; D055806:Microvessels / Q000254:growth & development*; D055262:Mucin-2 / Q000096:biosynthesis; D055272:Mucin-6 / Q000096:biosynthesis; D062048:Narrow Band Imaging; D015260:Neprilysin / Q000096:biosynthesis; D009940:Organoids / Q000254:growth & development / Q000473:pathology*; D013270:Stomach / Q000098:blood supply* / Q000473:pathology; D013274:Stomach Neoplasms / Q000098:blood supply* / Q000473:pathology
chemical_list: C524625:MUC2 protein, human; C524734:MUC6 protein, human; D055262:Mucin-2; D055272:Mucin-6; D015260:Neprilysin