title: Reaction products and the X-ray structure of AmpDh2, a virulence determinant of Pseudomonas aeruginosa.
abstract: The zinc protease AmpDh2 is a virulence determinant of Pseudomonas aeruginosa, a problematic human pathogen. The mechanism of how the protease manifests virulence is not known, but it is known that it turns over the bacterial cell wall. The reaction of AmpDh2 with the cell wall was investigated, and nine distinct turnover products were characterized by LC/MS/MS. The enzyme turns over both the cross-linked and noncross-linked cell wall. Three high-resolution X-ray structures, the apo enzyme and two complexes with turnover products, were solved. The X-ray structures show how the dimeric protein interacts with the inner leaflet of the bacterial outer membrane and that the two monomers provide a more expansive surface for recognition of the cell wall. This binding surface can accommodate the 3D solution structure of the cross-linked cell wall.
authors.affiliation: Department of Crystallography and Structural Biology, Inst. Química-Física "Rocasolano", CSIC, Serrano 119, 28006 Madrid, Spain.
authors.forename: Siseth
authors.initials: S
authors.lastname: Martínez-Caballero
authors.affiliation: Department of Chemistry and Biochemistry, Nieuwland Science Hall, Notre Dame, Indiana 46556, United States.
authors.forename: Mijoon
authors.initials: M
authors.lastname: Lee
authors.affiliation: Department of Crystallography and Structural Biology, Inst. Química-Física "Rocasolano", CSIC, Serrano 119, 28006 Madrid, Spain.
authors.forename: Cecilia
authors.initials: C
authors.lastname: Artola-Recolons
authors.affiliation: Department of Crystallography and Structural Biology, Inst. Química-Física "Rocasolano", CSIC, Serrano 119, 28006 Madrid, Spain.
authors.forename: César
authors.initials: C
authors.lastname: Carrasco-López
authors.affiliation: Department of Chemistry and Biochemistry, Nieuwland Science Hall, Notre Dame, Indiana 46556, United States.
authors.forename: Dusan
authors.initials: D
authors.lastname: Hesek
authors.affiliation: Department of Chemistry and Biochemistry, Nieuwland Science Hall, Notre Dame, Indiana 46556, United States.
authors.forename: Edward
authors.initials: E
authors.lastname: Spink
authors.affiliation: Department of Chemistry and Biochemistry, Nieuwland Science Hall, Notre Dame, Indiana 46556, United States.
authors.forename: Elena
authors.initials: E
authors.lastname: Lastochkin
authors.affiliation: Department of Chemistry and Biochemistry, Nieuwland Science Hall, Notre Dame, Indiana 46556, United States.
authors.forename: Weilie
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Department of Chemistry and Biochemistry, Nieuwland Science Hall, Notre Dame, Indiana 46556, United States.
authors.forename: Lance M
authors.initials: LM
authors.lastname: Hellman
authors.affiliation: Department of Chemistry and Biochemistry, Nieuwland Science Hall, Notre Dame, Indiana 46556, United States.
authors.forename: Bill
authors.initials: B
authors.lastname: Boggess
authors.affiliation: Department of Chemistry and Biochemistry, Nieuwland Science Hall, Notre Dame, Indiana 46556, United States.
authors.forename: Shahriar
authors.initials: S
authors.lastname: Mobashery
authors.affiliation: Department of Crystallography and Structural Biology, Inst. Química-Física "Rocasolano", CSIC, Serrano 119, 28006 Madrid, Spain.
authors.forename: Juan A
authors.initials: JA
authors.lastname: Hermoso
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry* / Q000378:metabolism; D018360:Crystallography, X-Ray; D045726:Metalloproteases / Q000737:chemistry* / Q000378:metabolism; D008958:Models, Molecular; D008968:Molecular Conformation; D015394:Molecular Structure; D011550:Pseudomonas aeruginosa / Q000201:enzymology*; D037521:Virulence Factors / Q000737:chemistry* / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D037521:Virulence Factors; C586328:AmpDh2 protein, Pseudomonas aeruginosa; D045726:Metalloproteases