title: V-ATPase Is Involved in Silkworm Defense Response against Bombyx mori Nucleopolyhedrovirus.
abstract: Silkworms are usually susceptible to the infection of Bombyx mori (B. mori) nucleopolyhedrovirus (BmNPV), which can cause significant economic loss. However, some silkworm strains are identified to be highly resistant to BmNPV. To explore the silkworm genes involved in this resistance in the present study, we performed comparative real-time PCR, ATPase assay, over-expression and sub-cellular localization experiments. We found that when inoculated with BmNPV both the expression and activity of V-ATPase were significantly up-regulated in the midgut column cells (not the goblet cells) of BmNPV-resistant strains (NB and BC8), the main sites for the first step of BmNPV invasion, but not in those of a BmNPV-susceptible strain 306. Furthermore, this up-regulation mainly took place during the first 24 hours post inoculation (hpi), the essential period required for establishment of virus infection, and then was down-regulated to normal levels. Amazingly, transient over-expression of V-ATPase c subunit in BmNPV-infected silkworm cells could significantly inhibit BmNPV proliferation. To our knowledge this is the first report demonstrating clearly that V-ATPase is indeed involved in the defense response against BmNPV. Our data further suggests that prompt and potent regulation of V-ATPase may be essential for execution of this response, which may enable fast acidification of endosomes and/or lysosomes to render them competent for degradation of invading viruses.
authors.affiliation: School of Food and Biological Engineering, Jiangsu University, Zhenjiang, Jiangsu, PR China ; Institute of Life Sciences, Jiangsu University, Zhenjiang, Jiangsu, PR China.
authors.forename: Peng
authors.initials: P
authors.lastname: Lü
authors.forename: Hengchuan
authors.initials: H
authors.lastname: Xia
authors.forename: Lu
authors.initials: L
authors.lastname: Gao
authors.forename: Ye
authors.initials: Y
authors.lastname: Pan
authors.forename: Yong
authors.initials: Y
authors.lastname: Wang
authors.forename: Xin
authors.initials: X
authors.lastname: Cheng
authors.forename: Honggang
authors.initials: H
authors.lastname: Lü
authors.forename: Feng
authors.initials: F
authors.lastname: Lin
authors.forename: Liang
authors.initials: L
authors.lastname: Chen
authors.forename: Qin
authors.initials: Q
authors.lastname: Yao
authors.forename: Xiaoyong
authors.initials: X
authors.lastname: Liu
authors.forename: Qi
authors.initials: Q
authors.lastname: Tang
authors.forename: Keping
authors.initials: K
authors.lastname: Chen
mesh_terms: D000818:Animals; D012831:Bombyx / Q000276:immunology / Q000821:virology*; D020869:Gene Expression Profiling; D017924:Nucleopolyhedroviruses / Q000472:pathogenicity* / Q000502:physiology; D060888:Real-Time Polymerase Chain Reaction; D025262:Vacuolar Proton-Translocating ATPases / Q000235:genetics / Q000502:physiology*; D014779:Virus Replication
chemical_list: D025262:Vacuolar Proton-Translocating ATPases