title: Cardiovascular effects of nose-only water-pipe smoking exposure in mice.
abstract: Water-pipe smoking (WPS) is a major type of smoking in Middle Eastern countries and is increasing in popularity in Western countries and is perceived as relatively safe. However, data on the adverse cardiovascular effects of WPS are scarce. Here, we assessed the cardiovascular effects of nose-only exposure to mainstream WPS generated by commercially available honey-flavored "moasel" tobacco in BALB/c mice. The duration of the session was 30 min/day for 1 mo. Control mice were exposed to air. WPS caused a significant increase of systolic blood pressure (SBP) in vivo (+13 mmHg) and plasma concentrations of IL-6 (+30%) but not that of TNF-α. Heart concentrations of IL-6 (+184%) and TNF-α (+54%) were significantly increased by WPS. Concentrations of ROS (+95%) and lipid peroxidation (+27%) were significantly increased, whereas those of GSH were decreased (-21%). WPS significantly shortened the thrombotic occlusion time in pial arterioles (-46%) and venules (40%). Plasma von Willebrand factor concentrations were significantly increased (+14%) by WPS. Erythrocyte numbers (+15%) and hematocrit (+17%) were significantly increased. Blood samples taken from mice exposed to WPS and exposed to ADP showed significant platelet aggregation compared with air-exposed mice. WPS caused a significant shortening of activated partial thromboplastin time (-45%) and prothrombin time (-13%). We conclude that 1-mo nose-only exposure to WPS increased SBP and caused cardiac inflammation, oxidative stress, and prothrombotic events. Our findings provide plausible elucidation that WPS is injurious to the cardiovascular system.
authors.affiliation: Department of Physiology, College of Medicine and Health Sciences, United Arab Emirates University, Al Ain, United Arab Emirates;
authors.forename: Abderrahim
authors.initials: A
authors.lastname: Nemmar
authors.forename: Priya
authors.initials: P
authors.lastname: Yuvaraju
authors.forename: Sumaya
authors.initials: S
authors.lastname: Beegam
authors.forename: Annie
authors.initials: A
authors.lastname: John
authors.forename: Haider
authors.initials: H
authors.lastname: Raza
authors.forename: Badreldin H
authors.initials: BH
authors.lastname: Ali
mesh_terms: D000280:Administration, Inhalation; D000818:Animals; D001794:Blood Pressure / Q000502:physiology; D002319:Cardiovascular System / Q000503:physiopathology*; D016207:Cytokines / Q000097:blood; D051379:Mice; D008807:Mice, Inbred BALB C; D023421:Models, Animal; D018384:Oxidative Stress / Q000502:physiology; D012907:Smoking / Q000009:adverse effects*; D014026:Nicotiana
chemical_list: D016207:Cytokines
keywords: heart; inflammation; nose-only exposure; oxidative stress; thrombosis; water-pipe smoking