title: Genetic and environmental influence on DNA strand break repair: a twin study.
abstract: Accumulation of DNA damage deriving from exogenous and endogenous sources has significant consequences for cellular survival, and is implicated in aging, cancer, and neurological diseases. Different DNA repair pathways have evolved in order to maintain genomic stability. Genetic and environmental factors are likely to influence DNA repair capacity. In order to gain more insight into the genetic and environmental contribution to the molecular basis of DNA repair, we have performed a human twin study, where we focused on the consequences of some of the most abundant types of DNA damage (single-strand breaks), and some of the most hazardous lesions (DNA double-strand breaks). DNA damage signaling response (Gamma-H2AX signaling), relative amount of endogenous damage, and DNA-strand break repair capacities were studied in peripheral blood mononuclear cells from 198 twins (94 monozygotic and 104 dizygotic). We did not detect genetic effects on the DNA-strand break variables in our study.
authors.affiliation: Danish Center of Molecular Gerontology and Danish Aging Research Center, Department of Molecular Biology and Genetics, University of Aarhus, Denmark.
authors.forename: Christian
authors.initials: C
authors.lastname: Garm
authors.forename: Maria
authors.initials: M
authors.lastname: Moreno-Villanueva
authors.forename: Alexander
authors.initials: A
authors.lastname: Bürkle
authors.forename: Lisbeth Aagaard
authors.initials: LA
authors.lastname: Larsen
authors.forename: Vilhelm A
authors.initials: VA
authors.lastname: Bohr
authors.forename: Kaare
authors.initials: K
authors.lastname: Christensen
authors.forename: Tinna
authors.initials: T
authors.lastname: Stevnsner
mesh_terms: D002470:Cell Survival / Q000235:genetics; D004247:DNA / Q000235:genetics; D053903:DNA Breaks, Double-Stranded; D053904:DNA Breaks, Single-Stranded; D004260:DNA Repair; D005260:Female; D006657:Histones / Q000378:metabolism*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000166:cytology; D008297:Male; D008875:Middle Aged; D014429:Twins, Dizygotic / Q000235:genetics*; D014430:Twins, Monozygotic / Q000235:genetics*
chemical_list: C468783:H2AX protein, human; D006657:Histones; D004247:DNA
keywords: DNA repair; double-strand break repair; gamma-H2AX; heritability; single-strand break repair; twins