title: Effect of Cell Shape and Dimensionality on Spindle Orientation and Mitotic Timing.
abstract: The formation and orientation of the mitotic spindle is a critical feature of mitosis. The morphology of the cell and the spatial distribution and composition of the cells' adhesive microenvironment all contribute to dictate the position of the spindle. However, the impact of the dimensionality of the cells' microenvironment has rarely been studied. In this study we present the use of a microwell platform, where the internal surfaces of the individual wells are coated with fibronectin, enabling the three-dimensional presentation of adhesive ligands to single cells cultured within the microwells. This platform was used to assess the effect of dimensionality and cell shape in a controlled microenvironment. Single HeLa cells cultured in circular microwells exhibited greater tilting of the mitotic spindle, in comparison to cells cultured in square microwells. This correlated with an increase in the time required to align the chromosomes at the metaphase plate due to prolonged activation of the spindle checkpoint in an actin dependent process. The comparison to 2D square patterns revealed that the dimensionality of cell adhesions alone affected both mitotic timings and spindle orientation; in particular the role of actin varied according to the dimensionality of the cells' microenvironment. Together, our data revealed that cell shape and the dimensionality of the cells' adhesive environment impacted on both the orientation of the mitotic spindle and progression through mitosis.
authors.affiliation: Laboratory for Surface Science and Technology, ETH Zurich, Zurich, Switzerland.
authors.forename: Mirren
authors.initials: M
authors.lastname: Charnley
authors.forename: Fabian
authors.initials: F
authors.lastname: Anderegg
authors.forename: René
authors.initials: R
authors.lastname: Holtackers
authors.forename: Marcus
authors.initials: M
authors.lastname: Textor
authors.forename: Patrick
authors.initials: P
authors.lastname: Meraldi
mesh_terms: D008841:Actin Cytoskeleton / Q000502:physiology; D048430:Cell Shape; D006367:HeLa Cells; D006801:Humans; D008938:Mitosis; D008941:Spindle Apparatus