title: Using immunoproteomics to identify tumor-associated antigens (TAAs) as biomarkers in cancer immunodiagnosis.
abstract: Since intracellular proteins involved in carcinogenesis have been shown to provoke autoantibody responses, autoantibodies can be used as probes in immunoproteomics to isolate, identify, and characterize potential tumor-associated antigens (TAAs). Once a TAA is identified, several approaches will be used to comprehensively characterize and validate the identified TAA/anti-TAA systems that are potential biomarkers in certain types of cancer. Our ultimate goal is to establish rigorous criteria for designation of an autoantibody to a TAA as a cancer biomarker, examine candidate TAAs for sensitivity and specificity of anti-TAA antibody response, and further develop customized TAA arrays that can be used to enhance anti-TAA antibody detection in cancer. This review will mainly focus on the recent advances in our studies using immunoproteomic approach to identify and characterize TAAs as biomarkers in cancer.
authors.affiliation: Department of Medical Oncology, The First Affiliated Hospital of Xi'an Jiao Tong University Medical Center, Xi'an, Shaanxi, China; Department of Biological Sciences, The University of Texas at El Paso, 500 West University Avenue, El Paso, TX, USA. Electronic address: newzhuqing1972@yahoo.com.
authors.forename: Qing
authors.initials: Q
authors.lastname: Zhu
authors.forename: Mei
authors.initials: M
authors.lastname: Liu
authors.forename: Liping
authors.initials: L
authors.lastname: Dai
authors.forename: Xia
authors.initials: X
authors.lastname: Ying
authors.forename: Hua
authors.initials: H
authors.lastname: Ye
authors.forename: Yusen
authors.initials: Y
authors.lastname: Zhou
authors.forename: Suxia
authors.initials: S
authors.lastname: Han
authors.forename: Jian-Ying
authors.initials: JY
authors.lastname: Zhang
mesh_terms: D000951:Antigens, Neoplasm / Q000276:immunology; D001323:Autoantibodies / Q000276:immunology; D015415:Biomarkers; D014408:Biomarkers, Tumor / Q000032:analysis*; D006801:Humans; D009369:Neoplasms / Q000175:diagnosis* / Q000276:immunology; D040901:Proteomics / Q000379:methods*; D012680:Sensitivity and Specificity
chemical_list: D000951:Antigens, Neoplasm; D001323:Autoantibodies; D015415:Biomarkers; D014408:Biomarkers, Tumor
keywords: Autoantibody; Cancer; Immunodiagnosis; Immunoproteomics; Tumor-associated antigen