title: Target identification of biologically active small molecules via in situ methods.
abstract: The identification of potential cellular targets of small molecules is important in biomedical research and drug discovery, but has been challenging due to a lack of proteome-based methods that enable direct investigation of small molecule-protein interaction in live cells. This review summarizes some of the recent advances in target identification of bioactive molecules (including drugs and natural products) using in situ methods for cell-based proteome profiling of potential on and off targets.
authors.affiliation: Department of Chemistry, National University of Singapore, 3 Science Drive 3, Singapore 117543, Republic of Singapore.
authors.forename: Ying
authors.initials: Y
authors.lastname: Su
authors.forename: Jingyan
authors.initials: J
authors.lastname: Ge
authors.forename: Biwei
authors.initials: B
authors.lastname: Zhu
authors.forename: Yu-Guo
authors.initials: YG
authors.lastname: Zheng
authors.forename: Qing
authors.initials: Q
authors.lastname: Zhu
authors.forename: Shao Q
authors.initials: SQ
authors.lastname: Yao
mesh_terms: D002470:Cell Survival; D015195:Drug Design; D006801:Humans; D020543:Proteome / Q000032:analysis; D040901:Proteomics; D054852:Small Molecule Libraries / Q000032:analysis*
chemical_list: D020543:Proteome; D054852:Small Molecule Libraries