title: Synergetic effect of SLN-curcumin and LDH-5-Fu on SMMC-7721 liver cancer cell line.
abstract: Curcumin and 5-Fluorouracil (5-Fu) have been reported to have anticancer potentials and show certain synergetic effect on some cancer cell lines. However, the poor bioavailability and rapid metabolism limited their medical application. In this study, we encapsulated curcumin with solid lipid nanoparticles (SLN), 5-Fu with Layered double hydroxides (LDHs) separately and tested its properties and anticancer potentials. SLN-curcumin and LDH-5-Fu were determined to be 100 and 60 nm by Transmission Electron Microscopy detection, and the loading efficiency were 28%±2.5% and 16.7%±1.8%, individually. Furthermore, SLN-curcumin and LDH-5-Fu showed a significantly synergetic effect on SMMC-7721 cell stronger than plain drugs together, of which the Idrug loaded nano-carriers was only 0.315. FACS analysis revealed that the combination of SLN-curcumin and LDH-5-Fu induced 80.1% apoptosis in SMMC-7721 cells, which were 1.7-folds of the sum of the two plain drug loaded carriers. The results demonstrated the significant synergetic anticancer potentials of nano-encapsulated curcumin and 5-Fu, which could be further explored for the treatment of other carcinoma.
authors.affiliation: 1 Tenth People's Hospital, School of Life Science and Technology, Tongji University , Shanghai, People's Republic of China .
authors.forename: Rongrong
authors.initials: R
authors.lastname: Zhu
authors.forename: Xianzheng
authors.initials: X
authors.lastname: Wu
authors.forename: Yu
authors.initials: Y
authors.lastname: Xiao
authors.forename: Bo
authors.initials: B
authors.lastname: Gao
authors.forename: Qian
authors.initials: Q
authors.lastname: Xie
authors.forename: Hui
authors.initials: H
authors.lastname: Liu
authors.forename: Shilong
authors.initials: S
authors.lastname: Wang
mesh_terms: D000971:Antineoplastic Combined Chemotherapy Protocols / Q000008:administration & dosage*; D017209:Apoptosis / Q000187:drug effects; D045744:Cell Line, Tumor; D003474:Curcumin / Q000008:administration & dosage* / Q000737:chemistry; D016503:Drug Delivery Systems; D004357:Drug Synergism; D005472:Fluorouracil / Q000008:administration & dosage* / Q000737:chemistry; D006801:Humans; D006878:Hydroxides / Q000008:administration & dosage / Q000737:chemistry; D008055:Lipids / Q000008:administration & dosage / Q000737:chemistry; D008113:Liver Neoplasms / Q000188:drug therapy* / Q000473:pathology; D053758:Nanoparticles / Q000008:administration & dosage*
chemical_list: D006878:Hydroxides; D008055:Lipids; D003474:Curcumin; D005472:Fluorouracil