title: Comparative efficiency of different pretreatment methods on enzymatic digestibility of Parthenium sp.
abstract: The potential of Parthenium sp. as a feedstock for enzymatic saccharification was investigated by using chemical and biological pretreatment methods. Mainly chemical pretreatments (acid and alkali) were compared with biological pretreatment with lignolytic fungi Marasmiellus palmivorus PK-27. Structural and chemical changes as well as crystallinity of cellulose were examined through scanning electron microscopy, fourier transform infra red and X-ray diffraction analysis, respectively after pretreatment. Total reducing sugar released during enzymatic saccharification of pretreated substrates was also evaluated. Among the pretreatment methods, alkali (1% NaOH) treated substrate showed high recovery of acid perceptible polymerised lignin (7.53 ± 0.5 mg/g) and significantly higher amount of reducing sugar (513.1 ± 41.0 mg/gds) compared to uninoculated Parthenium (163.4 ± 21.2) after 48 h of hydrolysis. This is the first report of lignolytic enzyme production from M. palmivorus, prevalent in oil palm plantations in Malaysia and its application in biological delignification of Parthenium sp. Alkali (1% NaOH) treatment proves to be the suitable method of pretreatment for lignin recovery and enhanced yield of reducing sugar which may be used for bioethanol production from Parthenium sp.
authors.affiliation: Division of Microbiology, Indian Agricultural Research Institute, New Delhi, 110012, India.
authors.forename: K
authors.initials: K
authors.lastname: Pandiyan
authors.forename: Rameshwar
authors.initials: R
authors.lastname: Tiwari
authors.forename: Sarika
authors.initials: S
authors.lastname: Rana
authors.forename: Anju
authors.initials: A
authors.lastname: Arora
authors.forename: Surender
authors.initials: S
authors.lastname: Singh
authors.forename: Anil Kumar
authors.initials: AK
authors.lastname: Saxena
authors.forename: Lata
authors.initials: L
authors.lastname: Nain
mesh_terms: D000363:Agaricales / Q000201:enzymology*; D019659:Asteraceae / Q000737:chemistry / Q000378:metabolism* / Q000648:ultrastructure; D002241:Carbohydrates / Q000032:analysis; D004798:Enzymes / Q000302:isolation & purification / Q000378:metabolism*; D005511:Food Handling / Q000379:methods*; D006868:Hydrolysis; D008031:Lignin / Q000032:analysis; D008296:Malaysia; D008855:Microscopy, Electron, Scanning; D017550:Spectroscopy, Fourier Transform Infrared; D014961:X-Ray Diffraction
chemical_list: D002241:Carbohydrates; D004798:Enzymes; D008031:Lignin