title: [Transgenic mouse models of the truncated platelet integrin β3 cytoplasmic tail established by stem cell transplantation].
abstract: This study was purpose to establish the transgenic mouse models of the truncated platelet integrin β3 by retrovirus-infected hematopoietic stem cells (HSCs) transplantation and to provide the basis for further study of the role of integrin β3 cytoplasmic domain in platelet bi-directional signaling pathways. Wild-type β3, β3-Δ759 (R(760) GT(762) truncated β3) and β3-Δ754 (T(755) NITYRGT(762) truncated β3) cDNAs were subcloned into MSCV MigR1 retroviral vector bearing a GFP gene and packaged into infective retrovirus with BOSC23 cell strain. The bone marrow HSCs of the β3 deficient mice were infected by the retroviruses, and transplanted into lethally-irradiated wild type C57BL/6 mice. GFP positive rate and surface β3 expression of the recipients' platelets at 6 to 8 weeks after transplantation were detected by flow cytometry to evaluate the transgenic efficiency. The results showed that four kinds of transgenic mouse models including vector, wild-type β3, β3-Δ759 and β3-Δ754 were established successfully. GFP positive rates of transgenic mouse platelets ranged from 18% to 66% and the β3 expression of transgenic mouse reached heterozygote (β3(+/-) level of mouse). It is concluded that establishment of transgenic mouse models mediated by retrovirus-infected HSCs transplantation is a feasible, fast, and high throughput transgenic approach and laid a solid foundation for further research on the role of integrin β3 cytoplasmic domain for bi-directional signaling of platelets in vivo, and for the gene therapy of platelet disorders.
authors.affiliation: Shanghai Jiaotong University School of Medicine, Shanghai, China.
authors.forename: Xiong-Ying
authors.initials: XY
authors.lastname: Cui
authors.forename: Xiao-Feng
authors.initials: XF
authors.lastname: Shi
authors.forename: Jian-Song
authors.initials: JS
authors.lastname: Huang
authors.forename: Ping
authors.initials: P
authors.lastname: Liu
authors.forename: Lan-Lan
authors.initials: LL
authors.lastname: Tao
authors.forename: Yu-Lan
authors.initials: YL
authors.lastname: Zhou
authors.forename: Zheng
authors.initials: Z
authors.lastname: Ruan
authors.forename: Xiao-Dong
authors.initials: XD
authors.lastname: Xi
mesh_terms: D000818:Animals; D001792:Blood Platelets / Q000378:metabolism*; D005822:Genetic Vectors; D018380:Hematopoietic Stem Cell Transplantation; D039661:Integrin beta3 / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D012190:Retroviridae / Q000235:genetics
chemical_list: D039661:Integrin beta3