title: [The natural triterpenoid miliacin prevents methotrexate-induced oxidative stress and normalizes the expression of genes encoding the cytochrome P-450 2E1 isoform and glutathione reductase in the liver].
abstract: We studied the role of the natural triterpenoid miliacin (3-3-methoxy-Al8-oleanene) in the regulation of oxidative stress in the liver of (CBAxC57B1(6))F1 mice exposed to methotrexate. Miliacin attenuated methotrexate-induced lipid peroxidation as determined by an attenuation of thiobarbituric acid-reacting products in the liver. Furthermore, miliacin normalized the expression of genes encoding the 2e1 isoform of cytochrome P-450 and glutathione reductase that were dramatically dysregulated by methotrexate. These results established the role of miliacin in modulation of redox genes, thereby providing evidence for a new mechanism of organ protection by this triterpenoid.
authors.affiliation: Blokhin Russian Cancer Research Center, RAMS, Moscow, Russia
authors.forename: O V
authors.initials: OV
authors.lastname: Kalinina
authors.forename: E S
authors.initials: ES
authors.lastname: Kolotova
authors.forename: T V
authors.initials: TV
authors.lastname: Panfilova
authors.forename: A A
authors.initials: AA
authors.lastname: Shtil
authors.forename: B A
authors.initials: BA
authors.lastname: Frolov
mesh_terms: D000818:Animals; D019392:Cytochrome P-450 CYP2E1 / Q000235:genetics / Q000378:metabolism*; D015870:Gene Expression / Q000187:drug effects; D005980:Glutathione Reductase / Q000235:genetics / Q000378:metabolism*; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism; D015227:Lipid Peroxidation; D008099:Liver / Q000201:enzymology*; D008297:Male; D008727:Methotrexate / Q000633:toxicity; D051379:Mice; D018384:Oxidative Stress / Q000187:drug effects*; D014315:Triterpenes / Q000494:pharmacology*
chemical_list: D007527:Isoenzymes; D014315:Triterpenes; C031972:miliacin; D019392:Cytochrome P-450 CYP2E1; D005980:Glutathione Reductase; D008727:Methotrexate