title: Differential control of Salmonella heat shock operons by structured mRNAs.
abstract: DnaK-DnaJ-GrpE and GroES-GroEL are the major chaperone machineries in bacteria. In many species, dnaKJ and groESL are encoded in bicistronic operons. Quantitative proteomics revealed that DnaK and GroEL amounts in Salmonella dominate over DnaJ and GroES respectively. An imperfect transcriptional terminator in the intergenic region of dnaKJ is known to result in higher transcript levels of the first gene. Here, we examined the groESL operon and asked how the second gene in a heat shock operon can be preferentially expressed and found that an RNA structure in the 5'untranslated region of groES is responsible. The secondary structure masks the Shine-Dalgarno (SD) sequence and AUG start codon and thereby modulates translation of groES mRNA. Reporter gene assays combined with structure probing and toeprinting analysis revealed a dynamic temperature-sensitive RNA structure. Following an increase in temperature, only the second of two RNA hairpins melts and partially liberates the SD sequence, thus facilitating translation. Translation of groEL is not temperature-regulated leading to an excess of the chaperonin in the cell at low temperature. Discussion in a broader context shows how structured RNA segments can differentially control expression of temperature-affected operons in various ways.
authors.affiliation: Lehrstuhl für Biologie der Mikroorganismen, Ruhr-Universität Bochum, 44780 Bochum, Germany.
authors.forename: Annika
authors.initials: A
authors.lastname: Cimdins
authors.forename: Johanna
authors.initials: J
authors.lastname: Roßmanith
authors.forename: Sina
authors.initials: S
authors.lastname: Langklotz
authors.forename: Julia E
authors.initials: JE
authors.lastname: Bandow
authors.forename: Franz
authors.initials: F
authors.lastname: Narberhaus
mesh_terms: D020121:5' Untranslated Regions; D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000235:genetics; D001483:Base Sequence; D018833:Chaperonins / Q000096:biosynthesis* / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D017930:Genes, Reporter; D006358:Hot Temperature; D008954:Models, Biological; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation / Q000528:radiation effects; D009876:Operon; D012333:RNA, Messenger / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D012486:Salmonella typhimurium / Q000235:genetics / Q000528:radiation effects*
chemical_list: D020121:5' Untranslated Regions; D001426:Bacterial Proteins; C097970:GroESL protein, Bacteria; D012333:RNA, Messenger; D018833:Chaperonins