title: Quantitative one- and two-dimensional 13C spectra of microcrystalline proteins with enhanced intensity.
abstract: We recorded quantitative, uniformly enhanced one- and two-dimensional (13)C spectra of labelled microcrystalline proteins. The approach takes advantage of efficient equilibration of magnetization by low-power proton irradiation using Phase Alternated Recoupling Irradiation Schemes and benefits simultaneously from uniform sensitivity enhancement due to efficient spin exchange that can overcome T1((13)C) constraints and the presence of heteronuclear Overhauser effects.
authors.affiliation: Département de Chimie, Ecole Normale Supérieure, 24 rue Lhomond, 75005, Paris, France.
authors.forename: Rudra N
authors.initials: RN
authors.lastname: Purusottam
authors.forename: Geoffrey
authors.initials: G
authors.lastname: Bodenhausen
authors.forename: Piotr
authors.initials: P
authors.lastname: Tekely
mesh_terms: D002247:Carbon Isotopes / Q000737:chemistry; D007553:Isotope Labeling; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D011487:Protein Conformation; D018080:Receptors, GABA-B / Q000737:chemistry*; D025801:Ubiquitin / Q000737:chemistry*
chemical_list: D002247:Carbon Isotopes; D018080:Receptors, GABA-B; D025801:Ubiquitin