title: Role of the zinc uptake ABC transporter of Moraxella catarrhalis in persistence in the respiratory tract.
abstract: Moraxella catarrhalis is a human respiratory tract pathogen that causes otitis media in children and lower respiratory tract infections in adults with chronic obstructive pulmonary disease. We have identified and characterized a zinc uptake ABC transporter that is present in all strains of M. catarrhalis tested. A mutant in which the znu gene cluster is knocked out shows markedly impaired growth compared to the wild type in medium that contains trace zinc; growth is restored to wild-type levels by supplementing medium with zinc but not with other divalent cations. Thermal-shift assays showed that the purified recombinant substrate binding protein ZnuA binds zinc but does not bind other divalent cations. Invasion assays with human respiratory epithelial cells demonstrated that the zinc ABC transporter of M. catarrhalis is critical for invasion of respiratory epithelial cells, an observation that is especially relevant because an intracellular reservoir of M. catarrhalis is present in the human respiratory tract and this reservoir is important for persistence. The znu knockout mutant showed marked impairment in its capacity to persist in the respiratory tract compared to the wild type in a mouse pulmonary clearance model. We conclude that the zinc uptake ABC transporter mediates uptake of zinc in environments with very low zinc concentrations and is critical for full virulence of M. catarrhalis in the respiratory tract in facilitating intracellular invasion of epithelial cells and persistence in the respiratory tract.
authors.affiliation: Clinical and Translational Research Center,, Division of Infectious Diseases, Department of Medicine, University at Buffalo, The State University of New York, Buffalo, New York, USA
authors.forename: Timothy F
authors.initials: TF
authors.lastname: Murphy
authors.forename: Aimee L
authors.initials: AL
authors.lastname: Brauer
authors.forename: Charmaine
authors.initials: C
authors.lastname: Kirkham
authors.forename: Antoinette
authors.initials: A
authors.lastname: Johnson
authors.forename: Mary
authors.initials: M
authors.lastname: Koszelak-Rosenblum
authors.forename: Michael G
authors.initials: MG
authors.lastname: Malkowski
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D004847:Epithelial Cells / Q000378:metabolism / Q000382:microbiology; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D001936:Moraxella catarrhalis / Q000235:genetics / Q000378:metabolism* / Q000472:pathogenicity; D045828:Moraxellaceae Infections / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D012137:Respiratory System / Q000378:metabolism / Q000382:microbiology; D012141:Respiratory Tract Infections / Q000235:genetics / Q000378:metabolism* / Q000382:microbiology; D014774:Virulence / Q000235:genetics; D015032:Zinc / Q000378:metabolism*
chemical_list: D018528:ATP-Binding Cassette Transporters; D002352:Carrier Proteins; D011994:Recombinant Proteins; C017800:zinc-binding protein; D015032:Zinc