title: Structure of the catalytic domain of protein tyrosine phosphatase sigma in the sulfenic acid form.
abstract: Protein tyrosine phosphatase sigma (PTPσ) plays a vital role in neural development. The extracellular domain of PTPσ binds to various proteoglycans, which control the activity of 2 intracellular PTP domains (D1 and D2). To understand the regulatory mechanism of PTPσ, we carried out structural and biochemical analyses of PTPσ D1D2. In the crystal structure analysis of a mutant form of D1D2 of PTPσ, we unexpectedly found that the catalytic cysteine of D1 is oxidized to cysteine sulfenic acid, while that of D2 remained in its reduced form, suggesting that D1 is more sensitive to oxidation than D2. This finding contrasts previous observations on PTPα. The cysteine sulfenic acid of D1 was further confirmed by immunoblot and mass spectrometric analyses. The stabilization of the cysteine sulfenic acid in the active site of PTP suggests that the formation of cysteine sulfenic acid may function as a stable intermediate during the redox-regulation of PTPs.
authors.affiliation: Department of Bioengineering, College of Engineering, Hanyang University, Seoul, 133-070, Korea.
authors.forename: Tae Jin
authors.initials: TJ
authors.lastname: Jeon
authors.forename: Pham Ngoc
authors.initials: PN
authors.lastname: Chien
authors.forename: Ha-Jung
authors.initials: HJ
authors.lastname: Chun
authors.forename: Seong Eon
authors.initials: SE
authors.lastname: Ryu
mesh_terms: D000595:Amino Acid Sequence; D020134:Catalytic Domain; D018360:Crystallography, X-Ray; D003545:Cysteine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D015151:Immunoblotting; D008958:Models, Molecular; D008969:Molecular Sequence Data; D050505:Mutant Proteins / Q000737:chemistry / Q000378:metabolism; D054623:Receptor-Like Protein Tyrosine Phosphatases, Class 2 / Q000737:chemistry* / Q000378:metabolism*; D012996:Solutions; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D055672:Static Electricity; D013434:Sulfenic Acids / Q000737:chemistry / Q000378:metabolism*
chemical_list: D050505:Mutant Proteins; D012996:Solutions; D013434:Sulfenic Acids; D054623:Receptor-Like Protein Tyrosine Phosphatases, Class 2; C100870:cysteinesulfenic acid; D003545:Cysteine