title: Expression analysis of multiple microRNAs in each patient with scleroderma.
abstract: In this study, we compared expression pattern of multiple microRNAs in individual patient with scleroderma with that in normal subject. Serum levels of six microRNAs (miR-7 g, miR-21, miR-29b, miR-125, miR-145 and miR-206) were evaluated using real-time PCR in 15 patients with scleroderma and 15 normal subjects. While levels of the six microRNAs were similar between the two groups, we found significant difference in the ranks between miRNAs in patients with scleroderma. Additionally, levels of let-7 g and miR-125b showed strong and significant correlation in normal subjects, but not in patients with scleroderma. Thus, miRNA expression pattern may be different in patients with scleroderma. We also found the combination of serum levels of miR-206 and miR-21 was more useful in distinguishing patients with scleroderma from normal subjects than either miR-206 or miR-21 alone. Our study is the first to demonstrate different expression profiles of multiple microRNAs in each patient with scleroderma and examine its clinical significance.
authors.forename: Shigeru
authors.initials: S
authors.lastname: Koba
authors.forename: Masatoshi
authors.initials: M
authors.lastname: Jinnin
authors.forename: Kuniko
authors.initials: K
authors.lastname: Inoue
authors.forename: Wakana
authors.initials: W
authors.lastname: Nakayama
authors.forename: Noritoshi
authors.initials: N
authors.lastname: Honda
authors.forename: Katsunari
authors.initials: K
authors.lastname: Makino
authors.forename: Ikko
authors.initials: I
authors.lastname: Kajihara
authors.forename: Takamitsu
authors.initials: T
authors.lastname: Makino
authors.forename: Satoshi
authors.initials: S
authors.lastname: Fukushima
authors.forename: Hironobu
authors.initials: H
authors.lastname: Ihn
mesh_terms: D019540:Area Under Curve; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006801:Humans; D035683:MicroRNAs / Q000378:metabolism*; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis; D012333:RNA, Messenger / Q000378:metabolism; D012372:ROC Curve; D060888:Real-Time Polymerase Chain Reaction; D045743:Scleroderma, Diffuse / Q000097:blood / Q000235:genetics*; D012595:Scleroderma, Systemic / Q000097:blood / Q000235:genetics*
chemical_list: D035683:MicroRNAs; D012333:RNA, Messenger; C531888:MIRN125 microRNA, human; C541010:MIRN145 microRNA, human; C546087:MIRN206 microRNA, human; C524940:MIRN21 microRNA, human; C532045:MIRN29a microRNA, human; C549242:MIRN7-1 microRNA, human