title: Biotinylation of silicon and nickel surfaces and detection of streptavidin as biosensor.
abstract: The availability of metal mesh device sensors has been investigated using surface-modified nickel mesh. Biotin was immobilized on the sensor surfaces consisting of silicon and nickel via a thiol-ene click reaction, known as the Michael addition reaction. Biotinylation on the maleimidated surface was confirmed by X-ray photoelectron spectroscopy. The binding of streptavidin to the biotinylated surfaces was evaluated using a quartz crystal microbalance and a metal mesh device sensor, with both techniques providing similar binding constant value. The recognition ability of the biotin immobilized using the thiol-maleimide method for streptavidin was comparable to that of biotin immobilized via several other methods. The adsorption of a biotin conjugate onto the streptavidin-immobilized surface via the biotin-streptavidin-biotin sandwich method was evaluated using a fluorescent microarray, with the results demonstrating that the biological activity of the streptavidin remained.
authors.affiliation: Graduate School of Engineering, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan.
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Seto
authors.forename: Chie
authors.initials: C
authors.lastname: Yamashita
authors.forename: Seiji
authors.initials: S
authors.lastname: Kamba
authors.forename: Takashi
authors.initials: T
authors.lastname: Kondo
authors.forename: Makoto
authors.initials: M
authors.lastname: Hasegawa
authors.forename: Mitsuhiro
authors.initials: M
authors.lastname: Matsuno
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Ogawa
authors.forename: Yu
authors.initials: Y
authors.lastname: Hoshino
authors.forename: Yoshiko
authors.initials: Y
authors.lastname: Miura
mesh_terms: D000327:Adsorption; D015374:Biosensing Techniques / Q000379:methods*; D001710:Biotin / Q000737:chemistry; D019921:Biotinylation; D006046:Gold / Q000737:chemistry; D055767:Immobilized Proteins / Q000737:chemistry; D008301:Maleimides / Q000737:chemistry; D049329:Nanostructures / Q000032:analysis; D009532:Nickel / Q000737:chemistry*; D012825:Silicon / Q000737:chemistry*; D019809:Streptavidin / Q000032:analysis* / Q000737:chemistry; D013499:Surface Properties
chemical_list: D055767:Immobilized Proteins; D008301:Maleimides; C043592:maleimide; D001710:Biotin; D006046:Gold; D009532:Nickel; D019809:Streptavidin; D012825:Silicon