title: HLA-B∗44 Is Associated with Dengue Severity Caused by DENV-3 in a Brazilian Population.
abstract: Human leukocyte antigen (HLA) alleles have been correlated with susceptibility or resistance to severe dengue; however, few immunogenetic studies have been performed in Latin American (LA) populations. We have conducted immunogenetic studies of HLA class I and II alleles in a cohort of 187 patients with DENV-3 infection and confirmed clinical diagnosis of either severe dengue, known as dengue hemorrhagic fever (DHF), or the less severe form, dengue fever (DF), in Recife, Pernambuco, Brazil. An association analysis was performed using Fisher's association test, with odds ratios (ORs) calculated using conditional maximum likelihood estimates. HLA-B∗44 (P = 0.047, OR = 2.025, 95% CI = 0.97-4.24) was found to be associated with increased susceptibility to DHF in response to DENV-3 infection. In addition, HLA-B∗07 (P = 0.048, OR = 0.501, one-sided 95% CI = 0-0.99) and HLA-DR∗13 (P = 0.028, OR = 0.511, one-sided 95% CI = 0-0.91) were found to be associated with resistance to secondary dengue infection by DENV-3. These results suggest that HLA-B∗44 supertype alleles and their respective T-cell responses might be involved in susceptibility to severe dengue infections, whereas the HLA-B∗07 supertype alleles and DR∗13 might be involved in cross-dengue serotype immunity.
authors.affiliation: Virology and Experimental Therapy Laboratory, Aggeu Magalhães Research Center, Fiocruz, Avenida Moraes Rego s/n, Campus da UFPE, Cidade Universitária, 50670-420 Recife, PE, Brazil.
authors.forename: Liciana
authors.initials: L
authors.lastname: Xavier Eurico de Alencar
authors.forename: Ulisses
authors.initials: U
authors.lastname: de Mendonça Braga-Neto
authors.forename: Eduardo
authors.initials: E
authors.lastname: José Moura do Nascimento
authors.forename: Marli
authors.initials: M
authors.lastname: Tenório Cordeiro
authors.forename: Ana
authors.initials: A
authors.lastname: Maria Silva
authors.forename: Carlos
authors.initials: C
authors.lastname: Alexandre Antunes de Brito
authors.forename: Maria da Paz Carvalho
authors.initials: Mda P
authors.lastname: da Silva
authors.forename: Laura Helena Vega Gonzales
authors.initials: LH
authors.lastname: Gil
authors.forename: Silvia Maria Lucena
authors.initials: SM
authors.lastname: Montenegro
authors.forename: Ernesto Torres de Azevedo
authors.initials: ET
authors.lastname: Marques