title: Complexes of silver(I) ions and silver phosphate nanoparticles with hyaluronic acid and/or chitosan as promising antimicrobial agents for vascular grafts.
abstract: Polymers are currently widely used to replace a variety of natural materials with respect to their favourable physical and chemical properties, and due to their economic advantage. One of the most important branches of application of polymers is the production of different products for medical use. In this case, it is necessary to face a significant disadvantage of polymer products due to possible and very common colonization of the surface by various microorganisms that can pose a potential danger to the patient. One of the possible solutions is to prepare polymer with antibacterial/antimicrobial properties that is resistant to bacterial colonization. The aim of this study was to contribute to the development of antimicrobial polymeric material ideal for covering vascular implants with subsequent use in transplant surgery. Therefore, the complexes of polymeric substances (hyaluronic acid and chitosan) with silver nitrate or silver phosphate nanoparticles were created, and their effects on gram-positive bacterial culture of Staphylococcus aureus were monitored. Stages of formation of complexes of silver nitrate and silver phosphate nanoparticles with polymeric compounds were characterized using electrochemical and spectrophotometric methods. Furthermore, the antimicrobial activity of complexes was determined using the methods of determination of growth curves and zones of inhibition. The results of this study revealed that the complex of chitosan, with silver phosphate nanoparticles, was the most suitable in order to have an antibacterial effect on bacterial culture of Staphylococcus aureus. Formation of this complex was under way at low concentrations of chitosan. The results of electrochemical determination corresponded with the results of spectrophotometric methods and verified good interaction and formation of the complex. The complex has an outstanding antibacterial effect and this effect was of several orders higher compared to other investigated complexes.
authors.affiliation: Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic. kizek@sci.muni.cz.
authors.forename: Dagmar
authors.initials: D
authors.lastname: Chudobova
authors.forename: Lukas
authors.initials: L
authors.lastname: Nejdl
authors.forename: Jaromir
authors.initials: J
authors.lastname: Gumulec
authors.forename: Olga
authors.initials: O
authors.lastname: Krystofova
authors.forename: Miguel Angel Merlos
authors.initials: MA
authors.lastname: Rodrigo
authors.forename: Jindrich
authors.initials: J
authors.lastname: Kynicky
authors.forename: Branislav
authors.initials: B
authors.lastname: Ruttkay-Nedecky
authors.forename: Pavel
authors.initials: P
authors.lastname: Kopel
authors.forename: Petr
authors.initials: P
authors.lastname: Babula
authors.forename: Vojtech
authors.initials: V
authors.lastname: Adam
authors.forename: Rene
authors.initials: R
authors.lastname: Kizek
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000737:chemistry / Q000494:pharmacology; D001807:Blood Vessel Prosthesis / Q000382:microbiology*; D002417:Cattle; D048271:Chitosan / Q000737:chemistry / Q000494:pharmacology; D006801:Humans; D006820:Hyaluronic Acid / Q000737:chemistry / Q000494:pharmacology; D007477:Ions / Q000737:chemistry; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010710:Phosphates / Q000737:chemistry / Q000494:pharmacology; D018030:Silver Compounds / Q000737:chemistry / Q000494:pharmacology; D013211:Staphylococcus aureus / Q000254:growth & development*
chemical_list: D000890:Anti-Infective Agents; D007477:Ions; D010710:Phosphates; D018030:Silver Compounds; D006820:Hyaluronic Acid; D048271:Chitosan; C039072:silver phosphate