title: The role of insulin-like growth factor 1 and its receptor in the formation and development of colorectal carcinoma.
abstract: OBJECTIVE
  To investigate the role of insulin-like growth factor (IGF)-1 and its receptor (IGF1R) in the formation and development of colorectal carcinoma.
  
  
  METHODS
  Colorectal tissue and matching serum samples were collected from patients with adenomatous polyps or carcinoma and healthy control subjects. IGF1R mRNA levels were determined via quantitative real-time reverse transcription-polymerase chain reaction. Serum IGF1 was quantified using enzyme-linked immunosorbent assay.
  
  
  RESULTS
  Serum IGF1 concentrations and mucosal IGF1R mRNA levels were significantly higher in patients with adenomatous polyps (n = 24) or carcinoma (n = 13) compared with healthy control subjects (n = 13). There was a significant positive correlation between serum IGF1 and mucosal IGF1R mRNA in patients with adenomatous polyps.
  
  
  CONCLUSIONS
  High circulating IGF1 concentrations and mucosal IGF1R expression may play important roles in both the formation and development of colorectal carcinoma. IGF1 and its receptor may be activated before carcinogenesis, and may promote the growth and malignant transformation of adenomatous polyps. IGF1 and IGF1R may be useful biomarkers for evaluating the stage and risk of carcinogenesis.
authors.affiliation: State Key Laboratory of Oncology in Southern China, Guangzhou, China.
authors.forename: Rong
authors.initials: R
authors.lastname: Zhang
authors.forename: Guo-Liang
authors.initials: GL
authors.lastname: Xu
authors.forename: Yin
authors.initials: Y
authors.lastname: Li
authors.forename: Long-Jun
authors.initials: LJ
authors.lastname: He
authors.forename: Li-Ming
authors.initials: LM
authors.lastname: Chen
authors.forename: Guo-Bao
authors.initials: GB
authors.lastname: Wang
authors.forename: Shi-Yong
authors.initials: SY
authors.lastname: Lin
authors.forename: Guang-Yu
authors.initials: GY
authors.lastname: Luo
authors.forename: Xiao-Yan
authors.initials: XY
authors.lastname: Gao
authors.forename: Hong-Bo
authors.initials: HB
authors.lastname: Shan
mesh_terms: D000230:Adenocarcinoma / Q000175:diagnosis / Q000235:genetics* / Q000378:metabolism; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000235:genetics* / Q000378:metabolism; D063646:Carcinogenesis / Q000235:genetics* / Q000378:metabolism; D016022:Case-Control Studies; D003110:Colonic Neoplasms / Q000175:diagnosis / Q000235:genetics* / Q000378:metabolism; D018450:Disease Progression; D005260:Female; D015870:Gene Expression; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000235:genetics* / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism; D017526:Receptor, IGF Type 1 / Q000235:genetics* / Q000378:metabolism
chemical_list: D014408:Biomarkers, Tumor; D012333:RNA, Messenger; D007334:Insulin-Like Growth Factor I; D017526:Receptor, IGF Type 1
keywords: Insulin-like growth factor 1; colorectal carcinoma; colorectal polyps; enzyme-linked immunosorbent assay; insulin-like growth factor receptor; quantitative real-time RT–PCR