title: Prostaglandin F2α upregulates Slit/Robo expression in mouse corpus luteum during luteolysis.
abstract: Prostaglandin F2 α (PGF2 α) is a key factor in the triggering of the regression of the corpus luteum (CL). Furthermore, it has been reported that Slit/Robo signaling is involved in the regulation of luteolysis. However, the interactions between PGF2 α and Slit/Robo in the progression of luteolysis remain to be established. This study was designed to determine whether luteolysis is regulated by the interactions of PGF2 α and Slit/Robo in the mouse CL. Real-time PCR and immunohistochemistry results showed that Slit2 and its receptor Robo1 are highly and specifically co-expressed in the mouse CL. Functional studies showed that Slit/Robo participates in mouse luteolysis by enhancing cell apoptosis and upregulating caspase3 expression. Both in vitro and in vivo studies showed that PGF2 α significantly increases the expression of Slit2 and Robo1 during luteolysis through protein kinase C-dependent ERK1/2 and P38 MAPK signaling pathways, whereas an inhibitor of Slit/Robo signaling significantly decreases the stimulating effect of PGF2 α on luteolysis. These findings indicate that Slit/Robo signaling plays important roles in PGF2 α-induced luteolysis by mediating the PGF2 α signaling pathway in the CL.
authors.affiliation: State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, No. 2 Yuanmingyuan Xilu, Beijing 100193, People's Republic of China.
authors.forename: Xuejing
authors.initials: X
authors.lastname: Zhang
authors.forename: Jianhua
authors.initials: J
authors.lastname: Li
authors.forename: Jiali
authors.initials: J
authors.lastname: Liu
authors.forename: Haoshu
authors.initials: H
authors.lastname: Luo
authors.forename: Kemian
authors.initials: K
authors.lastname: Gou
authors.forename: Sheng
authors.initials: S
authors.lastname: Cui
mesh_terms: D000818:Animals; D003338:Corpus Luteum / Q000254:growth & development / Q000378:metabolism*; D015237:Dinoprost / Q000378:metabolism*; D005260:Female; D036341:Intercellular Signaling Peptides and Proteins / Q000235:genetics* / Q000378:metabolism; D003341:Luteolysis; D051379:Mice; D009419:Nerve Tissue Proteins / Q000235:genetics* / Q000378:metabolism; D011971:Receptors, Immunologic / Q000235:genetics* / Q000378:metabolism; D015398:Signal Transduction; D015854:Up-Regulation; D000097905:Roundabout Proteins; D000098552:Slit Homolog 2 Protein
chemical_list: D015237:Dinoprost; D036341:Intercellular Signaling Peptides and Proteins; D009419:Nerve Tissue Proteins; D011971:Receptors, Immunologic; D000097905:Roundabout Proteins; D000098552:Slit Homolog 2 Protein
keywords: Slit/Robo; luteolysis; mouse; prostaglandin F2α