title: A genome-wide transcriptional profiling of sporulating Bacillus subtilis strain lacking PrpE protein phosphatase.
abstract: The sporulation process is a complex genetic developmental program leading to profound changes in global gene expression profile. In this work, we have applied genome-wide microarray approach for transcriptional profiling of Bacillus subtilis strain lacking a gene coding for PrpE protein phosphatase. This protein was previously shown to be involved in the regulation of germination of B. subtilis spores. Moreover, the deletion of prpE gene resulted in changing the resistance properties of spores. Our results provide genome-wide insight into the influence of this protein phosphatase on the physiology of B. subtilis cells. Although the precise role of PrpE in shaping the observed phenotype of ΔprpE mutant strain still remains beyond the understanding, our experiments brought observations of possible indirect implication of this protein in the regulation of cell motility and chemotaxis, as well as the development of competence. Surprisingly, prpE-deleted cells showed elevated level of general stress response, which turned out to be growth medium specific.
authors.affiliation: Laboratory of Molecular Bacteriology, Intercollegiate Faculty of Biotechnology, University of Gdańsk and Medical University of Gdańsk, Dębinki 1, 80-211, Gdańsk, Poland.
authors.forename: Adam
authors.initials: A
authors.lastname: Iwanicki
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Hinc
authors.forename: Anna
authors.initials: A
authors.lastname: Ronowicz
authors.forename: Arkadiusz
authors.initials: A
authors.lastname: Piotrowski
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Wołoszyk
authors.forename: Michał
authors.initials: M
authors.lastname: Obuchowski
mesh_terms: D001412:Bacillus subtilis / Q000235:genetics* / Q000502:physiology*; D016000:Cluster Analysis; D017353:Gene Deletion; D020869:Gene Expression Profiling; D016680:Genome, Bacterial / Q000235:genetics*; D046228:Microarray Analysis; D010749:Phosphoprotein Phosphatases / Q000172:deficiency; D013045:Species Specificity; D013171:Spores, Bacterial / Q000502:physiology; D001616:beta-Galactosidase / Q000378:metabolism
chemical_list: D010749:Phosphoprotein Phosphatases; D001616:beta-Galactosidase