title: Ocular fibroblast types differ in their mRNA profiles--implications for fibrosis prevention after aqueous shunt implantation.
abstract: OBJECTIVE
  For an aqueous shunt draining from the anterior chamber into the choroidal space, fibroblasts from the choroidea and/or the sclera are most likely responsible for a fibrotic response around the outflow region of such a shunt. The prevention of fibrosis should extend the operating life of the shunt. A detailed characterization of fibroblasts derived from choroidea and sclera should provide information about whether a fibrosis reaction can be inhibited by cell type-specific agents.
  
  
  METHODS
  We generated mRNA profiles of fibroblasts from the choroidea, sclera, and Tenon's space by gene array hybridization to provide a basis on which to search for potential pharmacological targets for fibrosis prevention. Hybridization data were analyzed by the Rosetta Resolver system and Limma to obtain mRNA profiles of the three fibroblast types.
  
  
  RESULTS
  The three fibroblast types investigated shared fibroblast-specific gene expression patterns concerning extracellular matrix proteins as collagens and fibronectin, but also showed distinct mRNA patterns.
  
  
  CONCLUSIONS
  Individual mRNA species overexpressed in one of the fibroblast types might serve as markers for the identification of the fibroblast type in histological analyses. Future in-depth analyses of the gene expression patterns might help identify pharmacological targets for fibrosis prevention.
authors.affiliation: Institut für Biomedizinische Technik, Universität Rostock, Rostock, Germany. marian.loebler@uni-rostock.de
authors.forename: Marian
authors.initials: M
authors.lastname: Löbler
authors.forename: Diana
authors.initials: D
authors.lastname: Buß
authors.forename: Christian
authors.initials: C
authors.lastname: Kastner
authors.forename: Jörg
authors.initials: J
authors.lastname: Mostertz
authors.forename: Georg
authors.initials: G
authors.lastname: Homuth
authors.forename: Mathias
authors.initials: M
authors.lastname: Ernst
authors.forename: Rudolf
authors.initials: R
authors.lastname: Guthoff
authors.forename: Andreas
authors.initials: A
authors.lastname: Wree
authors.forename: Thomas
authors.initials: T
authors.lastname: Stahnke
authors.forename: Georg
authors.initials: G
authors.lastname: Fuellen
authors.forename: Uwe
authors.initials: U
authors.lastname: Voelker
authors.forename: Klaus-Peter
authors.initials: KP
authors.lastname: Schmitz
mesh_terms: D024042:Collagen Type I / Q000235:genetics / Q000378:metabolism; D003238:Connective Tissue / Q000378:metabolism; D005109:Extracellular Matrix / Q000235:genetics; D005123:Eye / Q000473:pathology*; D005137:Eye, Artificial / Q000009:adverse effects*; D005347:Fibroblasts / Q000378:metabolism* / Q000473:pathology; D005355:Fibrosis; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D020327:Glaucoma Drainage Implants / Q000009:adverse effects*; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis; D009928:Organ Specificity / Q000235:genetics; D016133:Polymerase Chain Reaction; D025341:Principal Component Analysis; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
chemical_list: D024042:Collagen Type I; D012333:RNA, Messenger