title: Quantitative determination of d- and l-threo enantiomers of methylphenidate in brain tissue by liquid chromatography-mass spectrometry.
abstract: Methylphenidate, a psychostimulant used for the treatment of attention deficit hyperactivity disorder and narcolepsy, is administered as a 50:50 racemic mixture, despite the fact that d-methylphenidate has been shown to have greater pharmacologic activity. This paper presents a validated LC-MS/MS approach to separation and quantification of methylphenidate enantiomers using a vancomycin column and triethylammonium acetate to enhance the chiral separation. The method is applicable to the monitoring of these enantiomers in mouse brain, with a limit of detection of 0.5 ng/mL and a lower limit of quantification of 7.5 ng/mL.
authors.affiliation: Bill Gatton College of Pharmacy, East Tennessee State University, Pharmaceutical Sciences, Johnson City, Tennessee, USA.
authors.forename: Carolyn C
authors.initials: CC
authors.lastname: Combs
authors.forename: Erin L
authors.initials: EL
authors.lastname: Hankins
authors.forename: Cara L
authors.initials: CL
authors.lastname: Copeland
authors.forename: Stacy D
authors.initials: SD
authors.lastname: Brown
authors.forename: Brooks B
authors.initials: BB
authors.lastname: Pond
mesh_terms: D000818:Animals; D001923:Brain Chemistry; D002853:Chromatography, Liquid / Q000379:methods*; D057230:Limit of Detection; D008297:Male; D008774:Methylphenidate / Q000032:analysis* / Q000737:chemistry*; D051379:Mice; D015203:Reproducibility of Results; D013237:Stereoisomerism; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D008774:Methylphenidate
keywords: LC-MS; brain; chiral separation; methylphenidate