title: Insulin induces C2C12 cell proliferation and apoptosis through regulation of cyclin D1 and BAD expression.
abstract: Insulin is a secreted peptide hormone identified in human pancreas to promote glucose utilization. Insulin has been observed to induce cell proliferation and myogenesis in C2C12 cells. The precise mechanisms underlying the proliferation of C2C12 cells induced by insulin remain unclear. In this study, we observed for the first time that 10 nM insulin treatment promotes C2C12 cell proliferation. Additionally, 50 and 100 nM insulin treatment induces C2C12 cell apoptosis. By utilizing real-time PCR and Western blotting analysis, we found that the mRNA levels of cyclinD1 and BAD are induced upon 10 and 50 nM/100 nM insulin treatment, respectively. The similar results were observed in C2C12 cells expressing GATA-6 or PPARα. Our results identify for the first time the downstream targets of insulin, cyclin D1, and BAD, elucidate a new molecular mechanism of insulin in promoting cell proliferation and apoptosis.
authors.affiliation: Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Zhengzhou University, Zhengzhou City, Henan, 450001, China.
authors.forename: Cheng-Juan
authors.initials: CJ
authors.lastname: Xiong
authors.forename: Ping-Fa
authors.initials: PF
authors.lastname: Li
authors.forename: Yang-Liu
authors.initials: YL
authors.lastname: Song
authors.forename: Li-Xiang
authors.initials: LX
authors.lastname: Xue
authors.forename: Zhu-Qing
authors.initials: ZQ
authors.lastname: Jia
authors.forename: Chun-Xia
authors.initials: CX
authors.lastname: Yao
authors.forename: Qing-Xia
authors.initials: QX
authors.lastname: Wei
authors.forename: Shu-Feng
authors.initials: SF
authors.lastname: Zhang
authors.forename: Shan-Feng
authors.initials: SF
authors.lastname: Zhang
authors.forename: Yan-Yan
authors.initials: YY
authors.lastname: Zhang
authors.forename: Ji-Min
authors.initials: JM
authors.lastname: Zhao
authors.forename: Tian-Qi
authors.initials: TQ
authors.lastname: Wang
authors.forename: Mao-Feng
authors.initials: MF
authors.lastname: Guo
authors.forename: Ming-Xi
authors.initials: MX
authors.lastname: Zang
mesh_terms: D017209:Apoptosis / Q000235:genetics; D002460:Cell Line; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D019938:Cyclin D1 / Q000235:genetics*; D005434:Flow Cytometry; D050993:GATA6 Transcription Factor / Q000235:genetics / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007328:Insulin / Q000235:genetics*; D009369:Neoplasms / Q000235:genetics / Q000473:pathology; D047493:PPAR alpha / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction; D051025:bcl-Associated Death Protein / Q000235:genetics* / Q000378:metabolism
chemical_list: C494819:BAD protein, human; D050993:GATA6 Transcription Factor; D007328:Insulin; D047493:PPAR alpha; D051025:bcl-Associated Death Protein; D019938:Cyclin D1
keywords: BAD; CELL APOPTOSIS; CELL GROWTH; CYCLIN D1; INSULIN