title: The Effect of Z-Ligustilide on the Mobility of Human Glioblastoma T98G Cells.
abstract: Z-ligustilide (LIG), an essential oil extract from Radix Angelica sinensis, has broad pharmaceutical applications in treating cardio-vascular diseases and ischemic brain injury. Recently, LIG has been connected to Glioblastoma multiforme (GBM) because of its structural similarity to 3-n-alkyphthalide (NBP), which is specifically cytotoxic to GBM cells. Hence, we investigated LIG's effect on GBM T98G cells. The study shows that LIG can significantly reduce T98G cells' migration in a dose-dependent manner. Furthermore, the attenuation of cellular mobility can be linked to the activity of the Rho GTPases (RhoA, Rac1 and Cdc42), the three critical molecular switches governing cytoskeleton remodeling; thus, regulating cell migration. LIG significantly reduces the expression of RhoA and affects in a milder manner the expression of Cdc42 and Rac1.
authors.affiliation: Department of Chemical Engineering, University of Florida, Gainesville, Florida, United States of America ; National Cancer Institute - Physical Science Oncology Center, Gainesville, Florida, United States of America.
authors.forename: Jun
authors.initials: J
authors.lastname: Yin
authors.forename: Chengyuan
authors.initials: C
authors.lastname: Wang
authors.forename: Aaron
authors.initials: A
authors.lastname: Mody
authors.forename: Lin
authors.initials: L
authors.lastname: Bao
authors.forename: Shen-Hsiu
authors.initials: SH
authors.lastname: Hung
authors.forename: Spyros A
authors.initials: SA
authors.lastname: Svoronos
authors.forename: Yiider
authors.initials: Y
authors.lastname: Tseng
mesh_terms: D015107:4-Butyrolactone / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects*; D003599:Cytoskeleton / Q000378:metabolism / Q000473:pathology; D005909:Glioblastoma / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D009363:Neoplasm Proteins / Q000378:metabolism; D020764:cdc42 GTP-Binding Protein / Q000378:metabolism; D020830:rac1 GTP-Binding Protein / Q000378:metabolism; D020742:rhoA GTP-Binding Protein / Q000378:metabolism
chemical_list: D009363:Neoplasm Proteins; C107953:RAC1 protein, human; C066737:RHOA protein, human; C027820:ligustilide; D020764:cdc42 GTP-Binding Protein; D020830:rac1 GTP-Binding Protein; D020742:rhoA GTP-Binding Protein; D015107:4-Butyrolactone