title: Anxiolytic-like and antinociceptive effects of 2(S)-neoponcirin in mice.
abstract: OBJECTIVE
  2(S)-neopincirin (NEO) is a constituent from of Clinopodium mexicanum, which is used in traditional Mexican herbal medicine for its tranquilizing and analgesic properties. This study investigated the anxiolytic-like, sedative and antinociceptive effects of NEO in several mice models.
  
  
  METHODS
  The anxiolytic-like effect was evaluated in the hole-board (HBT) and Open Field Tests (OFT); sedative effect was evaluated in sleeping time induced by sodium pentobarbital, and its antinociceptive actions were measured in the hot plate test. To evaluate if the GABA receptor could be involved in the anxiolytic-like effect produced by NEO, in independent experiments, the effects produced by co-administration of NEO plus muscimol (MUS) and NEO plus Pitrotoxin (PTX) were evaluated in the HBT.
  
  
  RESULTS
  NEO was isolated from Clinopodium mexicanum leaves. The NMR, MS and optic rotation data helped establish its identity as (2S)-5-hydroxy-4'-methoxyflavanone-7-O-{β-glucopyranosyl-(1→6)-β-rhamnoside}. NEO showed an anxiolytic-like effect and was able to counter the nociception induced by a thermal stimulus in a dose-dependent manner. PTX blocked the anxiolytic-like effect of NEO, while MUS was able to enhance it.
  
  
  CONCLUSIONS
  The findings of present work demonstrated that NEO possesses anxiolytic-like and antinociceptive effects in mice. Such effects are not associated with changes in the locomotor activity. These results supported the notion that anxiolytic-like effect of NEO involves the participation of GABAergic system.
authors.affiliation: Departamento de Sistemas Biológicos, Universidad Autónoma Metropolitana Unidad Xochimilco, Mexico D.F. C.P. 04960, Mexico.
authors.forename: Julia
authors.initials: J
authors.lastname: Cassani
authors.forename: Anna G Escalona
authors.initials: AG
authors.lastname: Araujo
authors.forename: Mariano
authors.initials: M
authors.lastname: Martínez-Vázquez
authors.forename: Norberto
authors.initials: N
authors.lastname: Manjarrez
authors.forename: Julia
authors.initials: J
authors.lastname: Moreno
authors.forename: Rosa
authors.initials: R
authors.lastname: Estrada-Reyes
mesh_terms: D000700:Analgesics / Q000494:pharmacology*; D000818:Animals; D014151:Anti-Anxiety Agents / Q000494:pharmacology*; D001522:Behavior, Animal / Q000187:drug effects; D004338:Drug Combinations; D005419:Flavonoids / Q000494:pharmacology*; D018682:GABA Agents / Q000494:pharmacology; D006027:Glycosides / Q000494:pharmacology*; D029001:Herbal Medicine; D006993:Hypnotics and Sedatives / Q000494:pharmacology; D019686:Lamiaceae / Q000378:metabolism*; D008297:Male; D051379:Mice; D009043:Motor Activity / Q000187:drug effects; D009118:Muscimol / Q000494:pharmacology; D059225:Nociception / Q000187:drug effects*; D010936:Plant Extracts / Q000494:pharmacology; D012890:Sleep / Q000187:drug effects
chemical_list: C586216:2(S)-neoponcirin; D000700:Analgesics; D014151:Anti-Anxiety Agents; D004338:Drug Combinations; D005419:Flavonoids; D018682:GABA Agents; D006027:Glycosides; D006993:Hypnotics and Sedatives; D010936:Plant Extracts; D009118:Muscimol