title: Role of miR-19a targeting TNF-α in mediating ulcerative colitis.
abstract: OBJECTIVE
  Ulcerative colitis (UC) is a widely studied inflammatory disease associated with differential expression of genes involved in immune function, wound healing, and tissue remodeling. MicroRNAs have been reported to play a role in various cancer types. However, the mechanism of how microRNAs regulate UC remains unclear.
  
  
  METHODS
  In the present study, we investigated the role of miR-19a and tumor necrosis factor (TNF)-α in human colon tissues with UC and dextran sodium sulfate (DSS)-induced experimental colitis.
  
  
  RESULTS
  We identified that the expression of miR-19a was significantly reduced and TNF-α was remarkably increased in human colon tissue with UC. Moreover, this observation of miR-19a and TNF-α was also occurred in DSS-treated mice colitis. Further, we observed that miR-19a directly regulated TNF-α expression because miR-19a can suppress the expression of wild-type TNF-α reporter, but not the mutant form. The expression of inflammatory factors TNF-α, IL-8, and GM-GSF were significantly elevated upon application of miR-19a inhibitor.
  
  
  CONCLUSIONS
  Taken together, this study determines the levels of miR-19a and TNF-α in both DSS-induced experimental murine colitis and human UC and further demonstrates that miR-19a might directly regulate TNF-α. The findings may provide a new insight in the clinical treatment of UC.
authors.affiliation: Department of Gastroenterology, First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Chen
authors.forename: Shifeng
authors.initials: S
authors.lastname: She
authors.forename: Detang
authors.initials: D
authors.lastname: Li
authors.forename: Zhihui
authors.initials: Z
authors.lastname: Liu
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Yang
authors.forename: Zhirong
authors.initials: Z
authors.lastname: Zeng
authors.forename: Fengbin
authors.initials: F
authors.lastname: Liu
mesh_terms: D000328:Adult; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D015153:Blotting, Western; D016022:Case-Control Studies; D003093:Colitis, Ulcerative / Q000139:chemically induced / Q000235:genetics / Q000378:metabolism*; D003106:Colon / Q000378:metabolism*; D016264:Dextran Sulfate; D015536:Down-Regulation; D005260:Female; D019073:HT29 Cells; D006801:Humans; D007150:Immunohistochemistry; D007413:Intestinal Mucosa / Q000378:metabolism*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D035683:MicroRNAs / Q000378:metabolism*; D008875:Middle Aged; D060888:Real-Time Polymerase Chain Reaction; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*; D015854:Up-Regulation
chemical_list: D015415:Biomarkers; C549214:MIRN19A microRNA, human; C561642:MIRN19 microRNA, mouse; D035683:MicroRNAs; D014409:Tumor Necrosis Factor-alpha; D016264:Dextran Sulfate