title: Study on the interaction of the epilepsy drug, zonisamide with human serum albumin (HSA) by spectroscopic and molecular docking techniques.
abstract: In the present investigation, an attempt has been made to study the interaction of zonisamide (ZNS) with the transport protein, human serum albumin (HSA) employing UV-Vis, fluorometric, circular dichroism (CD) and molecular docking techniques. The results indicated that binding of ZNS to HSA caused strong fluorescence quenching of HSA through static quenching mechanism, hydrogen bonds and van der Waals contacts are the major forces in the stability of protein ZNS complex and the process of the binding of ZNS with HSA was driven by enthalpy (ΔH=-193.442 kJ mol(-1)). The results of CD and UV-Vis spectroscopy showed that the binding of this drug to HSA induced conformational changes in HSA. Furthermore, the study of molecular docking also indicated that zonisamide could strongly bind to the site I (subdomain IIA) of HSA mainly by hydrophobic interaction and there were hydrogen bond interactions between this drug and HSA, also known as the warfarin binding site.
authors.affiliation: Department of Inorganic Chemistry, Faculty of Chemistry, Razi University, Kermanshah, Iran. nahidshahabadi@yahoo.com
authors.forename: Nahid
authors.initials: N
authors.lastname: Shahabadi
authors.forename: Aref
authors.initials: A
authors.lastname: Khorshidi
authors.forename: Neda Hossinpour
authors.initials: NH
authors.lastname: Moghadam
mesh_terms: D000927:Anticonvulsants / Q000737:chemistry / Q000378:metabolism*; D002942:Circular Dichroism; D006801:Humans; D007555:Isoxazoles / Q000737:chemistry / Q000378:metabolism*; D062105:Molecular Docking Simulation; D011485:Protein Binding; D011487:Protein Conformation / Q000187:drug effects; D012709:Serum Albumin / Q000737:chemistry / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D013816:Thermodynamics; D000078305:Zonisamide
chemical_list: D000927:Anticonvulsants; D007555:Isoxazoles; D012709:Serum Albumin; D000078305:Zonisamide
keywords: Fluorescence; Human serum albumin (HSA); Molecular docking; Zonisamide