title: [Effect of Huperzine A on neural lesion of acute organophosphate poisoning in mice].
abstract: OBJECTIVE
  Effects of neurophathologic changes and expression of Glu and 60 nNOS were observed in acute isocarbophos and phoxim poisoning in mice.
  
  
  METHODS
  KM male mice were randomly divided into three groups, which were control, non-treated and Huperzine A (HupA)-treated groups. The control group was given tween-80. Nontreated group was given isocarbophos (14.7 mg/kg) or phoxim (1702 mg/kg). HupA-treated group was given HupA 2h before phoxim or isocarbophos. Twenty-four hours after exposure, the whole brain was removed and adjacent coronal sections was obtained. One part of sections were stained with toluidine blue. The part of sections were used to assessed the expression of Glu and nNOS in the cortex and hippocampal of brain by immunohistochemistry.
  
  
  RESULTS
  Compared to control group, non-treated group was observed nissal body nembers reduced and dyeing light. The animals of HupA protective group were observed nissal body nembers reduced, but the lesional degree was lighter obviously than non-treated group. The statistically reduced of the expression of Glu (P<0.01), the elevation of nNOS (P<0.01), after Isocarbophos intoxication were observed. Compared to non-treated group, the significant elevation of Glu (P<0.01) and reduced of nNOS (P<0.01) was observed on HupA-treated groups. Whereas for phoxim treatment, no changes were observed.
  
  
  CONCLUSIONS
  HupA have protective effect against glutamatergic systems disorder caused by Isocarbophos poisoning. Administration of HupA have no effects of the neurotransmitter changes induces by acute poisoning of phoxim. It is different for the toxic effect mechanism of the two organophosphate.
authors.affiliation: National Institute of Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention, Beijing, China.
authors.forename: Li
authors.initials: L
authors.lastname: Liu
authors.forename: Jian
authors.initials: J
authors.lastname: Wang
authors.forename: Guangyun
authors.initials: G
authors.lastname: Xie
authors.forename: Jinxiu
authors.initials: J
authors.lastname: Sun
mesh_terms: D000470:Alkaloids / Q000494:pharmacology*; D000818:Animals; D001921:Brain / Q000378:metabolism* / Q000473:pathology; D018698:Glutamic Acid / Q000378:metabolism; D008294:Malathion / Q000031:analogs & derivatives / Q000633:toxicity; D008297:Male; D051379:Mice; D018696:Neuroprotective Agents / Q000494:pharmacology*; D052248:Nitric Oxide Synthase Type I / Q000378:metabolism; D062025:Organophosphate Poisoning / Q000378:metabolism / Q000473:pathology*; D009946:Organothiophosphorus Compounds / Q000506:poisoning; D012717:Sesquiterpenes / Q000494:pharmacology*
chemical_list: D000470:Alkaloids; D018696:Neuroprotective Agents; D009946:Organothiophosphorus Compounds; D012717:Sesquiterpenes; C050426:huperzine A; D018698:Glutamic Acid; C003135:phoxim; C549713:isocarbophos; D052248:Nitric Oxide Synthase Type I; C496321:Nos1 protein, mouse; D008294:Malathion