title: Separation of mandelic acid and its derivatives with new immobilized cellulose chiral stationary phase.
abstract: A new liquid chromatographic method has been developed for the chiral separation of the enantiomers of mandelic acid and their derivatives 2-chloromandelic acid, 4-hydroxymandelic acid, 4-methoxymandelic acid, and 3,4,5-trismethoxymandelic acid. The enantiomers were separated by a CHIRALPAK(®) IC (250 mm×4.6 mm, 5 μm). Mandelic acid, 4-methoxymandelic acid, and 3,4,5-trismethoxymandelic acid were baseline resolved (resolution factor (RS)=2.21, RS=2.14, and RS=3.70, respectively). In contrast, the enantioselectivities between CHIRALPAK(®) IC and 2-chloromandelic acid and 4-hydroxymandelic acid investigated were low. By comparing the chromatographs of mandelic acid enantiomers and mandelic acid spiked with (R)-mandelic acid, it was determined that the first effluent was (R)-mandelic acid.
authors.affiliation: Key Laboratory of State Ministry of Education for Pharmaceutical Technology, School of Pharmacy, Zhengzhou University, Zhengzhou 450001, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Zhou
authors.forename: Qian
authors.initials: Q
authors.lastname: Liu
authors.forename: Guang-Jun
authors.initials: GJ
authors.lastname: Fu
authors.forename: Zhen-Zhong
authors.initials: ZZ
authors.lastname: Zhang
mesh_terms: D000438:Alcohols / Q000737:chemistry; D002482:Cellulose / Q000737:chemistry*; D002623:Chemistry Techniques, Analytical; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D008333:Mandelic Acids / Q000032:analysis* / Q000302:isolation & purification*; D013237:Stereoisomerism; D013696:Temperature; D014269:Trifluoroacetic Acid / Q000737:chemistry
chemical_list: C541344:2-chloromandelic acid; D000438:Alcohols; D008333:Mandelic Acids; C066949:4-methoxymandelic acid; C014600:4-hydroxymandelic acid; D002482:Cellulose; D014269:Trifluoroacetic Acid; C037938:mandelic acid