title: Reduction of cerebral Aβ burden and improvement in cognitive function in Tg-APPswe/PSEN1dE9 mice following vaccination with a multivalent Aβ3-10 DNA vaccine.
abstract: To develop a safe and efficient Aβ vaccine for Alzheimer's disease, we constructed a plasmid DNA vaccine encoding ten repeats of Aβ3-10 and three copies of C3d-p28 as a molecular adjuvant and administered it intramuscularly in 12-month-old female Tg-APPswe/PSEN1dE9 mice. Therapeutic immunization with p(Aβ3-10)10-C3d-p28.3 stimulated a Th2 immune response that elicited therapeutic levels of anti-Aβ antibodies and improved cognitive function. In addition, the vaccine reduced the cerebral Aβ burden and astrocytosis without increasing the incidence of microhemorrhage. Our results indicate that the p(Aβ3-10)10-C3d-p28.3 vaccine is a promising immunotherapeutic option for Aβ vaccination in Alzheimer's disease.
authors.affiliation: Department of Neurology, First Affiliated Hospital of China Medical University, No. 155 Nanjing North Street, Heping District, Shenyang 110001, Liao Ning Province, China.
authors.forename: Wanshu
authors.initials: W
authors.lastname: Guo
authors.forename: Sha
authors.initials: S
authors.lastname: Sha
authors.forename: Xiaona
authors.initials: X
authors.lastname: Xing
authors.forename: Tongzi
authors.initials: T
authors.lastname: Jiang
authors.forename: Yunpeng
authors.initials: Y
authors.lastname: Cao
mesh_terms: D000544:Alzheimer Disease / Q000235:genetics / Q000378:metabolism / Q000628:therapy*; D016229:Amyloid beta-Peptides / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000378:metabolism*; D049109:Cell Proliferation; D003071:Cognition; D003072:Cognition Disorders / Q000235:genetics / Q000378:metabolism / Q000628:therapy*; D018782:Maze Learning / Q000502:physiology; D051379:Mice; D008822:Mice, Transgenic; D013601:T-Lymphocytes; D014611:Vaccination; D019444:Vaccines, DNA / Q000627:therapeutic use*
chemical_list: D016229:Amyloid beta-Peptides; D019444:Vaccines, DNA
keywords: Alzheimer's disease; Amyloid-β; C3d-p28 molecular adjuvant; DNA vaccine