title: [Catalytic properties of enzymes from Erwinia carotovora involved in transamination of phenylpyruvate].
abstract: Km for L-phenylalanine, L-glutamic acid, L-aspartic acid, and the corresponding keto acids were calculated, as well as Vmax, was measured for the following pairs of substrates: L-phenylalanine-2-ketoglutarate, L-phenylalanine-oxaloacetate, L-glutamic acid-phenylpyruvate, and L-aspartic acid-phenylpyruvate for aminotransferases PATI, PAT2, and PAT3 from Erwinia carotovora catalyzing transamination of phenylpyruvate. The ping-pong bi-bi mechanism was shown for the studied aminotransferases. The substrate inhibition (Ks) of PAT3 with 2-ketoglutarate and oxaloacetate was 10.23 +/- 3.20 and 3.73 +/- 1.99 mM, respectively.
authors.forename: A M
authors.initials: AM
authors.lastname: Paloian
authors.forename: L A
authors.initials: LA
authors.lastname: Stepanian
authors.forename: S A
authors.initials: SA
authors.lastname: Dadaian
authors.forename: A A
authors.initials: AA
authors.lastname: Ambartsumian
authors.forename: G P
authors.initials: GP
authors.lastname: Alebian
authors.forename: A S
authors.initials: AS
authors.lastname: Sagian
mesh_terms: D000586:Amination; D001224:Aspartic Acid / Q000737:chemistry; D001426:Bacterial Proteins / Q000737:chemistry* / Q000302:isolation & purification; D055162:Biocatalysis; D018698:Glutamic Acid / Q000737:chemistry; D007527:Isoenzymes / Q000737:chemistry / Q000302:isolation & purification; D007656:Ketoglutaric Acids / Q000737:chemistry; D007700:Kinetics; D062907:Oxaloacetic Acid / Q000737:chemistry; D016973:Pectobacterium carotovorum / Q000737:chemistry* / Q000201:enzymology; D010649:Phenylalanine / Q000737:chemistry; D010667:Phenylpyruvic Acids / Q000737:chemistry*; D013379:Substrate Specificity; D000637:Transaminases / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: D001426:Bacterial Proteins; D007527:Isoenzymes; D007656:Ketoglutaric Acids; D010667:Phenylpyruvic Acids; D062907:Oxaloacetic Acid; D001224:Aspartic Acid; D018698:Glutamic Acid; D010649:Phenylalanine; D000637:Transaminases; C022340:phenylalanine aminotransferase; C031606:phenylpyruvic acid