title: The Bartonella henselae SitABCD transporter is required for confronting oxidative stress during cell and flea invasion.
abstract: Bartonella henselae is a zoonotic pathogen that possesses a flea-cat-flea transmission cycle and causes cat scratch disease in humans via cat scratches and bites. In order to establish infection, B. henselae must overcome oxidative stress damage produced by the mammalian host and arthropod vector. B. henselae encodes for putative Fe²⁺ and Mn²⁺ transporter SitABCD. In B. henselae, SitAB knockdown increases sensitivity to hydrogen peroxide. We consistently show that SitAB knockdown decreases the ability of B. henselae to survive in both human endothelial cells and cat fleas, thus demonstrating that the SitABCD transporter plays an important role during the B. henselae infection cycle.
authors.affiliation: Institute of Preventive Veterinary Medicine, Key Laboratory of Animal Disease and Human Health of Sichuan Province, Avian Disease Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu-611130/Ya'an-625014, Sichuan, PR China; Université Paris-Est, Ecole nationale vétérinaire d'Alfort, UMR BIPAR INRA-Anses-UPEC-ENVA, F-94700 Maisons-Alfort, France. Electronic address: liumafeng@yahoo.com.cn.
authors.forename: MaFeng
authors.initials: M
authors.lastname: Liu
authors.forename: Emilie
authors.initials: E
authors.lastname: Bouhsira
authors.forename: Henri-Jean
authors.initials: HJ
authors.lastname: Boulouis
authors.forename: Francis
authors.initials: F
authors.lastname: Biville
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000378:metabolism*; D018416:Bartonella henselae / Q000187:drug effects / Q000502:physiology*; D002413:Cations, Divalent / Q000378:metabolism; D002415:Cats; D002460:Cell Line; D058251:Ctenocephalides / Q000382:microbiology*; D042783:Endothelial Cells / Q000382:microbiology; D055785:Gene Knockdown Techniques; D006801:Humans; D006861:Hydrogen Peroxide / Q000633:toxicity; D007501:Iron / Q000378:metabolism; D008345:Manganese / Q000378:metabolism; D026901:Membrane Transport Proteins / Q000378:metabolism*; D050296:Microbial Viability / Q000187:drug effects; D018384:Oxidative Stress; D013312:Stress, Physiological
chemical_list: D001426:Bacterial Proteins; D002413:Cations, Divalent; D026901:Membrane Transport Proteins; D008345:Manganese; D006861:Hydrogen Peroxide; D007501:Iron
keywords: Bartonella; Flea transmission; Oxidative stress; SitABCD