title: Diallyl trisulfide suppresses dextran sodium sulfate-induced mouse colitis: NF-κB and STAT3 as potential targets.
abstract: Diallyl trisulfide (DATS), one of the volatile constituents of garlic oil, has been reported to possess antioxidant, anti-inflammatory, and anti-carcinogenic properties. In this study, DATS (10μmol) given orally for 7days before and for another 7days after starting administration of 2.5% dextran sulfate sodium (DSS) in drinking water protected against colitis induced by DSS in male ICR mice. DATS significantly inhibited the DSS-induced DNA binding of NF-κB, phosphorylation of IκBα and the expression of pro-inflammatory proteins, such as cyclooxygenase-2 and inducible nitric oxide synthase, which are major target proteins of NF-κB. The DSS-induced DNA binding and phosphorylation at the Tyr 705 residue of signal transducer and activator of transcription 3 (STAT3), and expression of its major target protein cyclin D1 in mouse colonic mucosa were also attenuated by DATS administration. Likewise, DSS-induced phosphorylation of extracellular signal-regulated kinase 1/2 was suppressed by DATS treatment. In conclusion, DATS ameliorates the DSS-induced mouse colitis presumably by blocking inflammatory signaling mediated by NF-κB and STAT3.
authors.affiliation: Tumor Microenvironment Global Core Research Institute, College of Pharmacy, Seoul National University, Seoul 151-742, South Korea.
authors.forename: Hyung-Jun
authors.initials: HJ
authors.lastname: Lee
authors.forename: Hee Geum
authors.initials: HG
authors.lastname: Lee
authors.forename: Ki-Seok
authors.initials: KS
authors.lastname: Choi
authors.forename: Young-Joon
authors.initials: YJ
authors.lastname: Surh
authors.forename: Hye-Kyung
authors.initials: HK
authors.lastname: Na
mesh_terms: D000498:Allyl Compounds / Q000494:pharmacology*; D000818:Animals; D003092:Colitis / Q000139:chemically induced*; D003911:Dextrans / Q000633:toxicity*; D051379:Mice; D016328:NF-kappa B / Q000187:drug effects* / Q000378:metabolism; D050796:STAT3 Transcription Factor / Q000187:drug effects* / Q000378:metabolism; D013440:Sulfides / Q000494:pharmacology*
chemical_list: D000498:Allyl Compounds; D003911:Dextrans; D016328:NF-kappa B; D050796:STAT3 Transcription Factor; C494087:Stat3 protein, mouse; D013440:Sulfides; C042577:diallyl trisulfide
keywords: Diallyl trisulfide; Mouse colitis; NF-κB; STAT3