title: Murine cell glycolipids customization by modular expression of glycosyltransferases.
abstract: Functional analysis of glycolipids has been hampered by their complex nature and combinatorial expression in cells and tissues. We report an efficient and easy method to generate cells with specific glycolipids. In our proof of principle experiments we have demonstrated the customized expression of two relevant glycosphingolipids on murine fibroblasts, stage-specific embryonic antigen 3 (SSEA-3), a marker for stem cells, and Forssman glycolipid, a xenoantigen. Sets of genes encoding glycosyltansferases were transduced by viral infection followed by multi-color cell sorting based on coupled expression of fluorescent proteins.
authors.affiliation: The ABO Histo-blood Groups and Cancer Laboratory, Cancer Genetics and Epigenetics Program, Institut de Medicina Predictiva i Personalitzada del Càncer (IMPPC), Badalona, Catalunya, Spain. ecid@imppc.org
authors.forename: Emili
authors.initials: E
authors.lastname: Cid
authors.forename: Miyako
authors.initials: M
authors.lastname: Yamamoto
authors.forename: Marcus
authors.initials: M
authors.lastname: Buschbeck
authors.forename: Fumiichiro
authors.initials: F
authors.lastname: Yamamoto
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D015870:Gene Expression; D006028:Glycosphingolipids / Q000378:metabolism; D016695:Glycosyltransferases / Q000096:biosynthesis* / Q000235:genetics; D057809:HEK293 Cells; D006801:Humans; D007790:Lactosylceramides / Q000378:metabolism*; D051379:Mice; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D012190:Retroviridae / Q000235:genetics
chemical_list: D015703:Antigens, CD; D006028:Glycosphingolipids; D007790:Lactosylceramides; D011994:Recombinant Proteins; C009744:CDw17 antigen; D016695:Glycosyltransferases