title: Correction for irrelevant absorption in multicomponent spectrophotometric assay of riboflavin, formylmethylflavin, and degradation products: kinetic applications.
abstract: In the spectrophotometric assay of multicomponent systems involved in drug degradation studies, some minor or unknown degradation products may be present. These products may interfere in the assay and thus invalidate the results due to their absorption in the range of analytical wavelengths. This interference may be eliminated by the application of an appropriate correction procedure to obtain reliable data for kinetic treatment. The present study is based on the application of linear and non-linear irrelevant absorption corrections in the multicomponent spectrophotometric assay of riboflavin and formylmethylflavin during the photolysis and hydrolysis studies. The correction procedures take into account the interference caused by minor or unknown products and have shown considerable improvement in the assay data in terms of the molar balance. The treatment of the corrected data has led to more accurate kinetic results in degradation studies.
authors.affiliation: Baqai Institute of Pharmaceutical Sciences, Baqai Medical University, Toll Plaza, Super Highway, Gadap Road, Karachi, 74600, Pakistan.
authors.forename: Iqbal
authors.initials: I
authors.lastname: Ahmad
authors.forename: Kiran
authors.initials: K
authors.lastname: Qadeer
authors.forename: Kefi
authors.initials: K
authors.lastname: Iqbal
authors.forename: Sofia
authors.initials: S
authors.lastname: Ahmed
authors.forename: Muhammad Ali
authors.initials: MA
authors.lastname: Sheraz
authors.forename: Syed Abid
authors.initials: SA
authors.lastname: Ali
authors.forename: Tania
authors.initials: T
authors.lastname: Mirza
authors.forename: Ambreen
authors.initials: A
authors.lastname: Hafeez
mesh_terms: D005415:Flavins / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D010782:Photolysis; D012256:Riboflavin / Q000737:chemistry*; D013053:Spectrophotometry / Q000379:methods*
chemical_list: C115829:10-formylmethylflavin; D005415:Flavins; D012256:Riboflavin