title: Characterization of a new zeaxanthin producing strain of Chlorella saccharophila isolated from New Zealand marine waters.
abstract: A fast growing strain of Chlorella saccharophila was isolated from the marine water of New Zealand and grown in heterotrophic conditions using glucose or glycerol as a carbon source. Biomass production was found to be higher in culture fed with glucose (2.14±0.08 g L(-1)) as compared to glycerol (0.378±0.04 g L(-1)). Lipid accumulation was similar for both carbon sources, at approximately 22% of dry cell weight. However, carotenoid yield was higher with glycerol (0.406±0.0125 mg g(-1)) than with glucose (0.21±0.034 mg g(-1)). Further optimization of the growth of the isolate gave maximal carotenoid production of 16.39±1.19 mg g(-1) total carotenoid, containing 11.32±0.64 mg g(-1) zeaxanthin and 5.07±0.55 mg g(-1) β-carotene. Comparison of various chemical and physical carotenoid extraction methods showed that ultrasonication was required for maximum extraction yields. The new strain has potential for biofuel, with carotenoid co-production.
authors.affiliation: Centre for Chemistry and Biotechnology, Waurn Ponds, Deakin University, Victoria 3217, Australia.
authors.forename: Dilip
authors.initials: D
authors.lastname: Singh
authors.forename: Munish
authors.initials: M
authors.lastname: Puri
authors.forename: Serena
authors.initials: S
authors.lastname: Wilkens
authors.forename: Anshu S
authors.initials: AS
authors.lastname: Mathur
authors.forename: Deepak K
authors.initials: DK
authors.lastname: Tuli
authors.forename: Colin J
authors.initials: CJ
authors.lastname: Barrow
mesh_terms: D001483:Base Sequence; D002708:Chlorella / Q000378:metabolism*; D017931:DNA Primers; D009520:New Zealand; D016133:Polymerase Chain Reaction; D012623:Seawater; D024341:Xanthophylls / Q000096:biosynthesis*; D065146:Zeaxanthins
chemical_list: D017931:DNA Primers; D024341:Xanthophylls; D065146:Zeaxanthins
keywords: Biofuel; Carotenoids; Microalgae; RP-HPLC; Ultrasonication