title: Isolation of gamma-interferon-inducible lysosomal thiol reductase (GILT) from the Yangtze finless porpoise.
abstract: In this study, we isolated the cDNA of a gamma-interferon-inducible lysosomal thiol reductase (GILT), which is critical for innate immune regulation, from the Yangtze finless porpoise (FpGILT). This gene encoded a protein with 244 amino acids and a predicted molecular weight of 28 kDa. The amino acid sequence of FpGILT includes an active-site CXXC motif, a GILT signature sequence, CQHGX2ECX2NX4C, and three N-linked glycosylation sites. Phylogenetic analysis showed that FpGILT and other GILT family members were derived from a common ancestor and finless porpoises are closely related to artiodactyla. Recombinant protein (FpsGILT) was then efficiently expressed and purified, and thiol reductase activity assays suggested that FpGILT catalyses disulfide bond reduction. These findings provide a basis for understanding the characteristics of immunity in the finless porpoise and other aquatic mammals.
authors.affiliation: Jiangsu Province Key Laboratory for Molecular and Medical Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing 210023, China.
authors.forename: Lidan
authors.initials: L
authors.lastname: Zhou
authors.forename: Weili
authors.initials: W
authors.lastname: Yan
authors.forename: Lin
authors.initials: L
authors.lastname: Yang
authors.forename: Hui
authors.initials: H
authors.lastname: Chen
authors.forename: Qianqian
authors.initials: Q
authors.lastname: Cao
authors.forename: Wenhua
authors.initials: W
authors.lastname: Ren
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D018076:DNA, Complementary / Q000235:genetics; D007371:Interferon-gamma / Q000276:immunology*; D008969:Molecular Sequence Data; D010088:Oxidoreductases / Q000235:genetics; D050862:Oxidoreductases Acting on Sulfur Group Donors / Q000235:genetics*; D010802:Phylogeny; D019576:Porpoises / Q000235:genetics*; D011994:Recombinant Proteins / Q000235:genetics; D017386:Sequence Homology, Amino Acid
chemical_list: D018076:DNA, Complementary; D011994:Recombinant Proteins; D007371:Interferon-gamma; D010088:Oxidoreductases; D050862:Oxidoreductases Acting on Sulfur Group Donors
keywords: Biological functions analysis; Finless porpoise; GILT; Gene cloning; Protein purification