title: Plasmodium berghei ANKA (PbA) infection of C57BL/6J mice: a model of severe malaria.
abstract: The term "severe malaria" refers to a wide spectrum of syndromes in Plasmodium-infected humans including cerebral malaria (CM), respiratory distress, severe anemia, liver dysfunction, and hypoglycemia. Mouse models have been employed to further our understanding of the pathology and immune responses that occur during Plasmodium infection. Evidence of brain, liver, lung, and spleen pathology, as well as anemia and tissue-sequestration of parasites, has been reported in various strains of inbred mice. While no single mouse model mimics all the various clinical manifestations of severe malaria in humans, here we describe a detailed protocol for Plasmodium berghei ANKA infection of C57BL/6J mice. For many years, this model has been referred to as "experimental cerebral malaria," but in fact recapitulates many of the symptoms and pathologies observed in most severe malaria syndromes.
authors.affiliation: Immunology and Infection Laboratory, Queensland Institute of Medical Research, Herston, QLD, Australia.
authors.forename: Marcela Montes
authors.initials: MM
authors.lastname: de Oca
authors.forename: Christian
authors.initials: C
authors.lastname: Engwerda
authors.forename: Ashraful
authors.initials: A
authors.lastname: Haque
mesh_terms: D000818:Animals; D001921:Brain / Q000469:parasitology / Q000473:pathology; D004195:Disease Models, Animal; D006801:Humans; D008099:Liver / Q000469:parasitology / Q000473:pathology; D008107:Liver Diseases / Q000469:parasitology / Q000473:pathology; D008168:Lung / Q000469:parasitology / Q000473:pathology; D008288:Malaria / Q000235:genetics* / Q000469:parasitology* / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D010962:Plasmodium berghei / Q000235:genetics / Q000472:pathogenicity*; D013154:Spleen / Q000469:parasitology / Q000473:pathology