title: Ionic-liquid based dispersive liquid-liquid microextraction followed by high performance liquid chromatographic determination of anti-hypertensives in rat serum.
abstract: A novel, simple and eco-friendly ionic liquid based dispersive liquid-liquid microextraction followed by HPLC determination of anti-hypertensive drugs viz., eprosartan, valasartan, irbesartan, losartan and telmisartan in rat serum has been developed and validated. Experimental parameters influencing the extraction efficiency, nature and volume of the ionic liquid, dispenser solvent, extraction time and effect of salt were optimized. Under the optimum conditions, the extraction recoveries were between 92.85 and 98.50%. The relative standard deviations of intra-and inter-day accuracy varied between 1.9 and 9.1% (n=3). The linearity of the proposed method was 0.1-20μg/mL with coefficients of determination varying between 0.9979 and 0.9992.
authors.affiliation: Analytical Chemistry Division, Indian Institute of Chemical Technology, Tarnaka, Hyderabad 500 007, India. rnrao@iict.res.in
authors.forename: R Nageswara
authors.initials: RN
authors.lastname: Rao
authors.forename: S Satyanarayana
authors.initials: SS
authors.lastname: Raju
authors.forename: R Mastan
authors.initials: RM
authors.lastname: Vali
mesh_terms: D000096:Acetone / Q000737:chemistry; D000818:Animals; D000959:Antihypertensive Agents / Q000097:blood* / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D007093:Imidazoles / Q000737:chemistry; D052578:Ionic Liquids / Q000737:chemistry*; D059627:Liquid Phase Microextraction / Q000379:methods*; D051381:Rats; D015203:Reproducibility of Results; D012965:Sodium Chloride / Q000737:chemistry
chemical_list: C412621:1-butyl-3-methylimidazolium hexafluorophosphate; D000959:Antihypertensive Agents; D007093:Imidazoles; D052578:Ionic Liquids; D000096:Acetone; D012965:Sodium Chloride