title: First report on circulation of Echinococcus ortleppi in the one humped camel (Camelus dromedaries), Sudan.
abstract: BACKGROUND
  Echinococcus granulosus (EG) complex, the cause of cystic echinococcosis (CE), infects humans and several other animal species worldwide and hence the disease is of public health importance. Ten genetic variants, or genotypes designated as (G1-G10), are distributed worldwide based on genetic diversity. The objective of this study was to provide some sequence data and phylogeny of EG isolates recovered from the Sudanese one-humped camel (Camelus dromedaries). Fifty samples of hydatid cysts were collected from the one- humped camels (Camelus dromedaries) at Taboul slaughter house, central Sudan. DNAs were extracted from protoscolices and/or associated germinal layers of hydatid cysts using a commercial kit. The mitochondrial NADH dehydrogenase subunit 1 (NADH1) gene and the cytochrome C oxidase subunit 1 (cox1) gene were used as targets for polymerase chain reaction (PCR) amplification. The PCR products were purified and partial sequences were generated. Sequences were further examined by sequence analysis and subsequent phylogeny to compare these sequences to those from known strains of EG circulating globally.
  
  
  RESULTS
  The identity of the PCR products were confirmed as NADH1 and cox1 nucleotide sequences using the Basic Local Alignment Search Tool (BLAST) of NCBI (National Center for Biotechnology Information, Bethesda, MD). The phylogenetic analysis showed that 98% (n = 49) of the isolates clustered with Echinococcus canadensis genotype 6 (G6), whereas only one isolate (2%) clustered with Echinococcus ortleppi (G5).
  
  
  CONCLUSIONS
  This investigation expands on the existing sequence data generated from EG isolates recovered from camel in the Sudan. The circulation of the cattle genotype (G5) in the one-humped camel is reported here for the first time.
authors.affiliation: Molecular Biology Laboratory, Faculty of Veterinary Medicine, University of Khartoum, Khartoum, Republic of the Sudan.
authors.forename: Mohamed E
authors.initials: ME
authors.lastname: Ahmed
authors.forename: Kamal H
authors.initials: KH
authors.lastname: Eltom
authors.forename: Nasreen O
authors.initials: NO
authors.lastname: Musa
authors.forename: Ibtisam A
authors.initials: IA
authors.lastname: Ali
authors.forename: Fatima M
authors.initials: FM
authors.lastname: Elamin
authors.forename: Martin P
authors.initials: MP
authors.lastname: Grobusch
authors.forename: Imadeldin E
authors.initials: IE
authors.lastname: Aradaib
mesh_terms: D000818:Animals; D001483:Base Sequence; D002162:Camelus / Q000469:parasitology*; D051545:Cyclooxygenase 1 / Q000235:genetics; D004443:Echinococcosis / Q000453:epidemiology / Q000469:parasitology / Q000662:veterinary*; D004446:Echinococcus / Q000235:genetics; D048209:Echinococcus granulosus / Q000235:genetics; D008969:Molecular Sequence Data; D009245:NADH Dehydrogenase / Q000235:genetics; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000662:veterinary; D016415:Sequence Alignment; D017421:Sequence Analysis / Q000662:veterinary; D013397:Sudan / Q000453:epidemiology
chemical_list: D051545:Cyclooxygenase 1; D009245:NADH Dehydrogenase