title: Scientific evaluation of the subchronic toxicity of Musca domestica larvae extracts in Sprague Dawley rats.
abstract: Musca domestica larvae extracts (MDLE) is a potential drug used to treat lipopolysaccharide-induced atherosclerosis pro-inflammatory responses. The purpose of the study was to evaluate the safety of MDLE via a 13-week repeated dose subchronic toxicity test in rats. Both male and female Sprague Dawley rats were divided into four groups, eight animals each from the control and high-dose group (33.0 g/kg) were allocated into recovery groups. The four groups of rats were administrated with MDLE (0, 13.2, 22.0, 33.0 g/kg) in the diet for 13weeks respectively. During the experimental period, the rats were observed for symptoms and signs of gross toxicity daily, food consumption and body weight were measured weekly. Urinalysis, thrombotest, blood biochemical and hematological analyses were performed regularly; Expression of peroxide dismutase gene in liver was quantified and a histopathological examination was also performed. There were no MDLE-induced abnormalities in any of the groups during or after the 13 weeks except the relative weight of liver of high-dose group and middle-dose group was significantly higher than that of control group in male rats (P<0.05). The results indicate a no observed adverse effect level for MDLE is 13.2 and 33.0 g/kg bw/day in male and female rats, respectively.
authors.affiliation: Guangdong Key Laboratory for Bioactive Drugs Research, Guangdong Pharmaceutical University, Guangzhou University City, Guangzhou, China.
authors.forename: Yun-Xi
authors.initials: YX
authors.lastname: Li
authors.forename: Xiao-Bao
authors.initials: XB
authors.lastname: Jin
authors.forename: Fu-Jiang
authors.initials: FJ
authors.lastname: Chu
authors.forename: Man-Yu
authors.initials: MY
authors.lastname: Liu
authors.forename: Da-Yu
authors.initials: DY
authors.lastname: Shi
authors.forename: Jia-Yong
authors.initials: JY
authors.lastname: Zhu
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000008:administration & dosage / Q000009:adverse effects*; D050197:Atherosclerosis / Q000188:drug therapy / Q000276:immunology; D001688:Biological Products / Q000008:administration & dosage / Q000009:adverse effects*; D004305:Dose-Response Relationship, Drug; D004353:Drug Evaluation, Preclinical; D020834:Ethnopharmacology; D005260:Female; D006529:Hepatomegaly / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology; D006793:Houseflies / Q000737:chemistry*; D007814:Larva / Q000737:chemistry; D008099:Liver / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008297:Male; D008516:Medicine, Chinese Traditional; D019055:No-Observed-Adverse-Effect Level; D009929:Organ Size / Q000187:drug effects; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D059550:Toxicity Tests, Subchronic
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D001688:Biological Products
keywords: Atherosclerosis; Musca domestica; Pro-inflammatory responses; Safety evaluation; Subchronic toxicity test