title: Glycerol and environmental factors: effects on 1,3-propanediol production and NAD(+) regeneration in Lactobacillus panis PM1.
abstract: OBJECTIVE
  This study was conducted to understand the influences of fermentation factors in NADH recycling and mechanisms of 1,3-propanediol (1,3-PDO) production in Lactobacillus panis PM1.
  
  
  RESULTS
  We conducted metabolite analyses, qRT-PCR of the glycerol reductive pathway [glycerol dehydratase (DhaB) and 1,3-PDO dehydrogenase (DhaT)] and DhaT activity assays at different pH, temperature and initial glycerol concentrations. The supplementation of 150 mmol l(-1) glycerol caused a shift in NADH flux from ethanol to 1,3-PDO production, whereas 300 mol l(-1) glycerol negatively affected the regeneration of NAD(+) via 1,3-PDO production. This retardation decreased transcription levels and specific activities of DhaT. The decreased DhaT activity eventually caused the shutdown of 1,3-PDO production. Temperature and pH did not significantly affect the specific activity of DhaT, whereas expression of genes for DhaB and DhaT was activated under acidic conditions. Moreover, fresh glucose addition after its depletion could not restart the glycerol reduction, but increased ethanol production.
  
  
  CONCLUSIONS
  Those environmental factors affect 1,3-PDO production in different ways through changing the expression level of enzymes and shifting the NAD(+) regeneration pathways.
  
  
  CONCLUSIONS
  Our findings elucidated a key element to optimize 1,3-PDO production by Lact. panis PM1, which potentially improves 1,3-PDO manufacturing efficiencies.
authors.affiliation: Department of Food and Bioproduct Sciences, College of Agriculture and Bioresources, University of Saskatchewan, Saskatoon, SK, Canada.
authors.forename: T S
authors.initials: TS
authors.lastname: Kang
authors.forename: D R
authors.initials: DR
authors.lastname: Korber
authors.forename: T
authors.initials: T
authors.lastname: Tanaka
mesh_terms: D005285:Fermentation; D005990:Glycerol / Q000378:metabolism*; D006836:Hydro-Lyases / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007778:Lactobacillus / Q000235:genetics / Q000378:metabolism*; D009243:NAD / Q000378:metabolism*; D011409:Propylene Glycols / Q000378:metabolism*; D013696:Temperature
chemical_list: D011409:Propylene Glycols; D009243:NAD; C041787:1,3-propanediol; D006836:Hydro-Lyases; C020671:glycerol dehydratase; D005990:Glycerol
keywords: PCR; biofuel; fermentation; lactic acid bacteria; metabolism