title: [Effect of ashitabe chalcones on the mRNA expression of PI3K and Akt in hepatocytes of rats with diabetes].
abstract: OBJECTIVE
  To study the effect of chalcones extracted from Angelica Keiskei (AC) on the mRNA expression of phosphatidy I inositol 3-kinase (PI3K) and serine-threonine kinases (Akt) in hepatocytes of rats with diabetes.
  
  
  METHODS
  The diabetes of rats was induced by streptozotocin with intraperitoeal injection as well as with high-fat diet feeding. The rats were randomly divided into 3 groups with 10 rats in each group,diabetic control group, high-dose AC group and low-dose AC group. All the rats were fed with high-fat diet. 0, 30 and 10 mg/kg BW AC per day were given to high-dose AC, low-dose AC and the diabetic control groups,respectively. Another 10 normal rats fed with regular diet were used as the normal control group. After 4 weeks, serum insulin levels were evaluated by radioimmunoassay. The mRNA expression levels of PI3K and Akt were determined by reverse transcription polymerase chain reaction (RT-PCR). The phosphorylation levels of Akt in hepatocytes were detected by western blot. Blood glucose levels were measured by glucose oxidase method.
  
  
  RESULTS
  Compared with rats in the diabetic control gruop, the levels of blood glucose and serum insulin in rats of high-dose AC gruop were decreased and the PI3K and AKT mRNA expression levels were increased. All the difference was statistically significant (P<0.05).
  
  
  CONCLUSIONS
  AC may upregulate the mRNA expression levels of PI3K and Akt and improve insulin resistance of rats with diabetes.
authors.affiliation: Institute of Nutrition, Medical College of Qingdao University, Qingdao 266021, China. liubei19871105@sina.com
authors.forename: Bei
authors.initials: B
authors.lastname: Liu
authors.forename: Jianping
authors.initials: J
authors.lastname: Sun
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhao
authors.forename: Lei
authors.initials: L
authors.lastname: Li
authors.forename: Jinyi
authors.initials: J
authors.lastname: Zhong
mesh_terms: D029969:Angelica / Q000737:chemistry*; D000818:Animals; D047188:Chalcones / Q000494:pharmacology*; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism*; D003924:Diabetes Mellitus, Type 2 / Q000378:metabolism; D022781:Hepatocytes / Q000378:metabolism; D007333:Insulin Resistance; D008297:Male; D019869:Phosphatidylinositol 3-Kinases / Q000235:genetics / Q000378:metabolism*; D051057:Proto-Oncogene Proteins c-akt / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D015854:Up-Regulation
chemical_list: D047188:Chalcones; D012333:RNA, Messenger; D019869:Phosphatidylinositol 3-Kinases; D051057:Proto-Oncogene Proteins c-akt