title: In vitro human adipose-derived stromal/stem cells osteogenesis in akermanite:poly-ε-caprolactone scaffolds.
abstract: This study compared the metabolic activity, cell proliferation and osteogenic differentiation of human adipose-derived stromal/stem cells cultured on four different scaffolds (poly-ε-caprolactone, akermanite:poly-ε-caprolactone composites, akermanite and β-tricalcium phosophate) with or without osteogenic media supplementation for up to 21 days. The hypothesis was that human adipose-derived stromal/stem cells osteogenesis in akermanite-containing scaffolds would be greater than the other scaffold types independent of the media supplementation. According to the results, human adipose-derived stromal/stem cells loaded on different scaffolds and cultured in both media conditions displayed significant changes in the metabolic activity and cell proliferation. After 21 days of culture in osteogenic medium, the human adipose-derived stromal/stem cells loaded onto akermanite-based scaffolds had greater calcium deposition and osteocalcin expression relative to human adipose-derived stromal/stem cells loaded onto β-tricalcium phosophate and poly-ε-caprolactone. In vivo investigations are needed to further assess the bone tissue engineering potential of human adipose-derived stromal/stem cells loaded to akermanite:poly-ε-caprolactone composites.
authors.affiliation: 1Department of Biological Engineering, Louisiana State University and LSU AgCenter, Baton Rouge, LA, USA.
authors.forename: Andrea S
authors.initials: AS
authors.lastname: Zanetti
authors.forename: Gregory T
authors.initials: GT
authors.lastname: McCandless
authors.forename: Julia Y
authors.initials: JY
authors.lastname: Chan
authors.forename: Jeffrey M
authors.initials: JM
authors.lastname: Gimble
authors.forename: Daniel J
authors.initials: DJ
authors.lastname: Hayes
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000328:Adult; D049109:Cell Proliferation; D002516:Ceramics; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D010012:Osteogenesis; D011091:Polyesters; D060888:Real-Time Polymerase Chain Reaction; D013234:Stem Cells / Q000166:cytology*; D017154:Stromal Cells / Q000166:cytology*; D054457:Tissue Scaffolds
chemical_list: D011091:Polyesters; C016240:polycaprolactone; C023506:Glass ceramics
keywords: Akermanite; bone; human adipose-derived stromal/stem cells; osteogenesis; poly-ε-caprolactone; scaffolds