title: Thiobencarb herbicide reduces growth, photosynthetic activity, and amount of Rieske iron-sulfur protein in the diatom Thalassiosira pseudonana.
abstract: We investigated the effects of the herbicide thiobencarb on the growth, photosynthetic activity, and expression profile of photosynthesis-related proteins in the marine diatom Thalassiosira pseudonana. Growth rate was suppressed by 50% at a thiobencarb concentration of 1.26 mg/L. Growth and photosystem II activity (Fv /Fm ratio) were drastically decreased at 5 mg/L, at which the expression levels of 13 proteins increased significantly and those of 11 proteins decreased significantly. Among these proteins, the level of the Rieske iron-sulfur protein was decreased to less than half of the control level. This protein is an essential component of the cytochrome b6 f complex in the photosynthetic electron transport chain. Although the mechanism by which thiobencarb decreased the Rieske iron-sulfur protein level is not clear, these results suggest that growth was inhibited by interruption of the photosynthetic electron transport chain by thiobencarb.
authors.affiliation: Faculty of Agriculture, Kyushu University, Hakozaki 6-10-1, Higashi-ku, Fukuoka, 812-8581, Japan. simasaki@agr.kyushu-u.ac.jp
authors.forename: Yohei
authors.initials: Y
authors.lastname: Shimasaki
authors.forename: Michito
authors.initials: M
authors.lastname: Tsuyama
authors.forename: Rumana
authors.initials: R
authors.lastname: Tasmin
authors.forename: Xuchun
authors.initials: X
authors.lastname: Qiu
authors.forename: Motoyuki
authors.initials: M
authors.lastname: Shimizu
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Sato
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Yamasaki
authors.forename: Yoko
authors.initials: Y
authors.lastname: Kato-Unoki
authors.forename: Atsushi
authors.initials: A
authors.lastname: Nukata
authors.forename: Takashi
authors.initials: T
authors.lastname: Nakashima
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Ichinose
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Wariishi
authors.forename: Tsuneo
authors.initials: T
authors.lastname: Honjo
authors.forename: Yuji
authors.initials: Y
authors.lastname: Oshima
mesh_terms: D000595:Amino Acid Sequence; D017377:Diatoms / Q000187:drug effects*; D014450:Electron Transport Complex III / Q000378:metabolism; D006540:Herbicides / Q000494:pharmacology*; D010084:Oxidation-Reduction; D010788:Photosynthesis / Q000187:drug effects*; D013859:Thiocarbamates / Q000494:pharmacology*
chemical_list: D006540:Herbicides; C037989:Rieske iron-sulfur protein; D013859:Thiocarbamates; C037925:benthiocarb; D014450:Electron Transport Complex III
keywords: Proteomics; Rieske Iron-Sulfur Protein; Thalassiosira pseudonana; Thiobencarb