title: Uptake of cadmium by hydroponically grown, mature Eucalyptus camaldulensis saplings and the effect of organic ligands.
abstract: The potential suitability of Eucalyptus camaldulensis for Cd phytoextraction was tested in a hydroponic study. Saplings were exposed to 4.5 and 89 microM Cd for one month, with and without EDTA and s,s-EDDS at 0.1, 1, and 5 mM. The saplings' growth was not affected at the 4.5 microM Cd concentration, yet it decreased 3-fold at 89 microM, and almost all the Cd taken up was immobilized in the roots, reaching 360 and 5300 mg Cd kg(-1), respectively (approximately 75% of which was non-washable in acid). The respective Cd root-to-shoot translocation factors were 0.14 and approximately 5*10(-4). At 0.1 mM concentration, EDTA and EDDS had no effect or even a positive effect on the saplings growth. This was reversed at 1 mM, and the chelants became lethal at the 5 mM concentration. At 89 microM Cd in the growth medium, 0.1 mM EDTA increased Cd translocation into the shoots by almost 10-fold, however it strongly reduced Cd content inside the roots. This hydroponic study indicates the feasibility of E. camaldulensis use for cleanup Cd-contaminated soils at environmental concentrations, both for site stabilization (phytostabilization) and gradual remediation (phytoextraction). EDTA was shown to be much more efficient in enhancing Cd translocation than s,s-EDDS.
authors.affiliation: Institute of Soil, Water and Environmental Sciences, Volcani Center, ARO, Bet-Dagan, Israel. finep@volcani.agri.gov.il
authors.forename: P
authors.initials: P
authors.lastname: Fine
authors.forename: Paresh H
authors.initials: PH
authors.lastname: Rathod
authors.forename: A
authors.initials: A
authors.lastname: Beriozkin
authors.forename: U
authors.initials: U
authors.lastname: Mingelgrin
mesh_terms: D000042:Absorption; D001673:Biodegradation, Environmental; D001692:Biological Transport / Q000187:drug effects; D018533:Biomass; D002104:Cadmium / Q000032:analysis / Q000378:metabolism*; D004492:Edetic Acid / Q000494:pharmacology*; D005029:Ethylenediamines / Q000494:pharmacology*; D005052:Eucalyptus / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism*; D018527:Hydroponics; D008024:Ligands; D018517:Plant Roots / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D018520:Plant Shoots / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D012989:Soil Pollutants / Q000378:metabolism; D013386:Succinates / Q000494:pharmacology*; D014131:Trace Elements / Q000032:analysis / Q000378:metabolism
chemical_list: C499100:(S,S)-ethylenediaminedisuccinate; D005029:Ethylenediamines; D008024:Ligands; D012989:Soil Pollutants; D013386:Succinates; D014131:Trace Elements; D002104:Cadmium; D004492:Edetic Acid