title: Lack of association between ESR1 gene polymorphisms and premature ovarian failure in Serbian women.
abstract: OBJECTIVE
  It has previously been reported that estrogen receptor-alpha (ERα) gene (ESR1: estrogen receptor 1) polymorphisms are associated with premature ovarian failure (POF). The aim of this study was to investigate whether these genetic polymorphisms of ESR1 are associated with POF in Serbian women.
  
  
  METHODS
  A series of 197 POF cases matched with 547 fertile controls was recruited by the Institute for Endocrinology, Diabetes and Metabolic Disorders of Serbia between 2007 and 2010. Genomic DNA was extracted from saliva using Oragene® DNA sample collection kits. Two single-nucleotide polymorphisms (SNPs), PvuII and XbaI, in ESR1 were genotyped by dynamic allele-specific hybridization. Haplotype analyses were performed with the restriction fragment length polymorphism method. SNP and haplotype effects were analyzed by logistic regression models.
  
  
  RESULTS
  No significant difference was found in the distribution of ESR1 PvuII and XbaI polymorphisms or haplotypes between the POF and control groups.
  
  
  CONCLUSIONS
  The two ESR1 SNPs, PvuII and XbaI, are not commonly associated with POF in Serbian women and may not contribute to the genetic basis of the condition.
authors.affiliation: * Gynaecology Research Unit, University Hospitals of Leicester , Leicester , UK.
authors.forename: J
authors.initials: J
authors.lastname: Li
authors.forename: S
authors.initials: S
authors.lastname: Vujovic
authors.forename: R
authors.initials: R
authors.lastname: Dalgleish
authors.forename: J
authors.initials: J
authors.lastname: Thompson
authors.forename: S
authors.initials: S
authors.lastname: Dragojevic-Dikic
authors.forename: F
authors.initials: F
authors.lastname: Al-Azzawi
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D047628:Estrogen Receptor alpha / Q000235:genetics*; D005260:Female; D006239:Haplotypes; D006801:Humans; D020641:Polymorphism, Single Nucleotide; D016649:Primary Ovarian Insufficiency / Q000235:genetics*; D055771:Serbia; D055815:Young Adult
chemical_list: C506487:ESR1 protein, human; D047628:Estrogen Receptor alpha
keywords: ESR1; ESTROGEN RECEPTOR ALPHA; GENE POLYMORPHISMS; PREMATURE OVARIAN FAILURE