title: Expression strategy of Aedes albopictus densovirus.
abstract: The transcription map of the Aedes albopictus densovirus (AalDNV) brevidensovirus was identified by Northern blotting, rapid amplification of cDNA ends (RACE) analysis, and RNase protection assays. AalDNV produced mRNAs of 3,359 (NS1), 3,345 (NS2), and 1,246 (VP) nucleotides. The two overlapping P7/7.4 NS promoters employed closely located alternate transcription initiation sites, positioned at either side of the NS1 initiation codon. All NS mRNAs coterminated with VP mRNA. All promoters, explored using luciferase assays, were functional in insect and human cell lines.
authors.affiliation: INRS-Institut Armand-Frappier, Laval, Québec, Canada.
authors.forename: Hanh T
authors.initials: HT
authors.lastname: Pham
authors.forename: Françoise-Xavière
authors.initials: FX
authors.lastname: Jousset
authors.forename: Jonathan
authors.initials: J
authors.lastname: Perreault
authors.forename: Hiroko
authors.initials: H
authors.lastname: Shike
authors.forename: Jozsef
authors.initials: J
authors.lastname: Szelei
authors.forename: Max
authors.initials: M
authors.lastname: Bergoin
authors.forename: Peter
authors.initials: P
authors.lastname: Tijssen
mesh_terms: D000330:Aedes / Q000821:virology*; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D017990:Densovirus / Q000235:genetics*; D015870:Gene Expression; D016679:Genome, Viral; D006801:Humans; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000235:genetics; D012367:RNA, Viral / Q000235:genetics; D024363:Transcription Initiation Site; D017361:Viral Nonstructural Proteins / Q000235:genetics; D014764:Viral Proteins / Q000235:genetics
chemical_list: D012333:RNA, Messenger; D012367:RNA, Viral; D017361:Viral Nonstructural Proteins; D014764:Viral Proteins