title: Hepatocyte growth on polycapronolactone and 2-hydroxyethylmethacrylate nanofiber sheets enhanced by bone marrow-derived mesenchymal stromal cells.
abstract: OBJECTIVE
  The development of hepatocyte-based Bioartificial Liver Assist Devices, intended for the therapy of chronic and fulminant liver failure, is one of the important tasks in the area of tissue engineering. New advances in the development of semipermeable non-woven nanofiber biomaterials and the co-cultivation of bone marrow mesenchymal stromal cells (BMSC) and hepatocytes could be utilized in order to maintain hepatocyte cultures in these devices.
  
  
  METHODS
  We have compared rat hepatocyte growth on nanofiber biomaterials from different polymers, 2-hydroxyethylmethacrylate (HEMA) and ethoxyethylmethacrylate (EOEMA) copolymers, polyurethane (PUR), chitosan and polycapronolactone (PCL) spun from different solvent mixtures.
  
  
  RESULTS
  In all cases the adhesion of hepatocytes to nanofibers was significantly better/stronger than to unstructured polymer surfaces; coating the nanofibers with collagen did not increase cell adhesion. We found the best hepatocyte adhesion on HEMA/EOEMA copolymer nanofibers and PCL nanofibers spun from a mixture of ethylacetate and dimethyl sulphoxide. Using a migration assay, we observed the migration of BMSC towards hepatocytes; hepatocytes cocultivated with BMSC excreted lower amounts of stress enzymes.
  
  
  CONCLUSIONS
  The results demonstrate that nonwoven nanofiber layers, particularly those containing BMSC, are a suitable biocompatible support for functional hepatocyte cultures and that they can be used in a laboratory bioreactor or potentially in clinical setting.
authors.forename: Dana
authors.initials: D
authors.lastname: Mareková
authors.forename: Petr
authors.initials: P
authors.lastname: Lesný
authors.forename: Pavla
authors.initials: P
authors.lastname: Jendelová
authors.forename: Jiří
authors.initials: J
authors.lastname: Michálek
authors.forename: Petra
authors.initials: P
authors.lastname: Kostecká
authors.forename: Martin
authors.initials: M
authors.lastname: Přádný
authors.forename: Lenka
authors.initials: L
authors.lastname: Martinová
authors.forename: Tomáš
authors.initials: T
authors.lastname: Pantoflíček
authors.forename: Miroslav
authors.initials: M
authors.lastname: Ryska
authors.forename: Eva
authors.initials: E
authors.lastname: Syková
mesh_terms: D000818:Animals; D049109:Cell Proliferation; D018920:Coculture Techniques; D022781:Hepatocytes / Q000502:physiology*; D019164:Liver, Artificial; D059630:Mesenchymal Stem Cells / Q000502:physiology*; D008689:Methacrylates / Q000494:pharmacology*; D057139:Nanofibers / Q000627:therapeutic use*; D011091:Polyesters / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D008689:Methacrylates; D011091:Polyesters; C016240:polycaprolactone; C005044:hydroxyethyl methacrylate