title: Neuromuscular disease classification system.
abstract: Diagnosis of neuromuscular diseases is based on subjective visual assessment of biopsies from patients by the pathologist specialist. A system for objective analysis and classification of muscular dystrophies and neurogenic atrophies through muscle biopsy images of fluorescence microscopy is presented. The procedure starts with an accurate segmentation of the muscle fibers using mathematical morphology and a watershed transform. A feature extraction step is carried out in two parts: 24 features that pathologists take into account to diagnose the diseases and 58 structural features that the human eye cannot see, based on the assumption that the biopsy is considered as a graph, where the nodes are represented by each fiber, and two nodes are connected if two fibers are adjacent. A feature selection using sequential forward selection and sequential backward selection methods, a classification using a Fuzzy ARTMAP neural network, and a study of grading the severity are performed on these two sets of features. A database consisting of 91 images was used: 71 images for the training step and 20 as the test. A classification error of 0% was obtained. It is concluded that the addition of features undetectable by the human visual inspection improves the categorization of atrophic patterns.
authors.affiliation: University of Seville, Department of Signal Theory and Communications, ETSI, 41092, Seville, Spain. aurorasaez@us.es
authors.forename: Aurora
authors.initials: A
authors.lastname: Sáez
authors.forename: Begoña
authors.initials: B
authors.lastname: Acha
authors.forename: Adoración
authors.initials: A
authors.lastname: Montero-Sánchez
authors.forename: Eloy
authors.initials: E
authors.lastname: Rivas
authors.forename: Luis M
authors.initials: LM
authors.lastname: Escudero
authors.forename: Carmen
authors.initials: C
authors.lastname: Serrano
mesh_terms: D000465:Algorithms; D001284:Atrophy; D001706:Biopsy / Q000379:methods; D016208:Databases, Factual; D003936:Diagnosis, Computer-Assisted / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D008856:Microscopy, Fluorescence / Q000379:methods*; D015233:Models, Statistical; D018482:Muscle, Skeletal / Q000473:pathology; D009132:Muscles / Q000473:pathology; D016571:Neural Networks, Computer; D009468:Neuromuscular Diseases / Q000145:classification* / Q000175:diagnosis* / Q000473:pathology; D015203:Reproducibility of Results