title: Analysis of microglial production of reactive oxygen and nitrogen species.
abstract: Reactive oxygen and nitrogen species are both regulators and effectors of microglial activation, and assays of these oxidants can be used as a measure of acute and chronic activation of microglial cells. Here we describe quick methods to assess the production of superoxide, hydrogen peroxide, nitric oxide, and peroxynitrite by microglia.
authors.affiliation: University of Cambridge, Cambridge, UK.
authors.forename: Urte
authors.initials: U
authors.lastname: Neniskyte
authors.forename: Guy C
authors.initials: GC
authors.lastname: Brown
mesh_terms: D000818:Animals; D006801:Humans; D017628:Microglia / Q000378:metabolism*; D009569:Nitric Oxide / Q000378:metabolism; D030421:Peroxynitrous Acid; D026361:Reactive Nitrogen Species / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D013481:Superoxides / Q000378:metabolism
chemical_list: D026361:Reactive Nitrogen Species; D017382:Reactive Oxygen Species; D013481:Superoxides; D030421:Peroxynitrous Acid; D009569:Nitric Oxide