title: High-throughput screening using dengue virus reporter genomes.
abstract: Large-scale antiviral drug discovery and systematic identification of host factors promoting or restricting virus replication require robust, scalable, and cost-effective assay systems that allow for high throughput and automation. Here we describe the construction and application of full-length infectious dengue virus reporter genomes for such screening applications.
authors.affiliation: Haplogen GmbH, Vienna, Austria.
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Fischl
authors.forename: Ralf
authors.initials: R
authors.lastname: Bartenschlager
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology*; D018929:Cell Culture Techniques; D002460:Cell Line; D002474:Cell-Free System; D002522:Chlorocebus aethiops; D003001:Cloning, Molecular; D006224:Cricetinae; D018076:DNA, Complementary / Q000235:genetics; D003716:Dengue Virus / Q000187:drug effects* / Q000235:genetics*; D017930:Genes, Reporter; D016679:Genome, Viral; D057166:High-Throughput Screening Assays / Q000379:methods; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods*; D014158:Transcription, Genetic; D014709:Vero Cells; D014779:Virus Replication / Q000187:drug effects
chemical_list: D000998:Antiviral Agents; D018076:DNA, Complementary