title: Protective effect of Pinus koraiensis needle water extract against oxidative stress in HepG2 cells and obese mice.
abstract: Needles of pine species are rich in polyphenols, which may exert beneficial effects on human health. The present study was conducted to evaluate the in vitro and in vivo antioxidant effects of Pinus koraiensis needle water extracts (PKW). HepG2 cells were pretreated with various concentrations of PKW (from 10(-3) to 1 mg/mL) and oxidative stress was induced by tert-butyl hydroperoxide (t-BOOH). In the animal model, male ICR mice were fed a high-fat diet for 6 weeks to induce obesity, and then mice were continually fed a high-fat diet with or without orally administered PKW (400 mg/kg body weight) for 5 weeks. Pretreatment with PKW prevented significant increases in cytotoxicity and catalase activity induced by t-BOOH in HepG2 cells. Similarly, the catalase protein expression levels elevated by t-BOOH were abrogated in cells pretreated with PKW. In mice fed a high-fat diet, PKW significantly increased hepatic activities of catalase and glutathione reductase and lower lipid peroxidation levels were observed in the liver and kidney of mice with PKW supplementation. The present study demonstrates that PKW protects against oxidative stress in HepG2 cells treated with t-BOOH and in mice fed a high-fat diet.
authors.affiliation: Department of Food and Nutrition, Seoul National University, Seoul, Korea.
authors.forename: Sae Bom
authors.initials: SB
authors.lastname: Won
authors.forename: Ga-young
authors.initials: GY
authors.lastname: Jung
authors.forename: Juhae
authors.initials: J
authors.lastname: Kim
authors.forename: Young Shin
authors.initials: YS
authors.lastname: Chung
authors.forename: Eun Kyung
authors.initials: EK
authors.lastname: Hong
authors.forename: Young Hye
authors.initials: YH
authors.lastname: Kwon
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage; D002374:Catalase / Q000235:genetics / Q000378:metabolism; D005980:Glutathione Reductase / Q000378:metabolism; D056945:Hep G2 Cells; D006801:Humans; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D008820:Mice, Obese; D009765:Obesity / Q000188:drug therapy / Q000235:genetics / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D028223:Pinus / Q000737:chemistry*; D010936:Plant Extracts / Q000008:administration & dosage*; D018515:Plant Leaves / Q000737:chemistry
chemical_list: D000975:Antioxidants; D010936:Plant Extracts; D002374:Catalase; D005980:Glutathione Reductase