title: Molecular and biological characterization of corchorus mottle virus, a new begomovirus from Brazil.
abstract: A begomovirus infecting Orinoco jute (Corchorus hirtus) from Brazil was characterized. Molecular analysis revealed a bipartite genomic organization, which is typical of the New World begomoviruses. Sequence analysis and phylogenetic data showed that both genomic components have the closest relationship with abutilon mosaic Brazil virus, with an identity of 87.3 % for DNA-A, indicating that this virus is a member of a new begomovirus species for which the name "Corchorus mottle virus" (CoMoV) is proposed. Sida rhombifolia plants inoculated by biolistics with an infectious clone of CoMoV showed systemic vein chlorosis, mottling and leaf deformation symptoms, while Nicotiana benthamiana and tomato plants had symptomless infection. CoMoV is the first corchorus-infecting begomovirus reported in Brazil.
authors.affiliation: Laboratório de Interação Planta-Praga III, Embrapa Recursos Genéticos e Biotecnologia, Pq. Estação Biológica, Brasília, DF, 70770-917, Brazil.
authors.forename: Rosana
authors.initials: R
authors.lastname: Blawid
authors.forename: Rafaela S
authors.initials: RS
authors.lastname: Fontenele
authors.forename: Cristiano
authors.initials: C
authors.lastname: Lacorte
authors.forename: Simone G
authors.initials: SG
authors.lastname: Ribeiro
mesh_terms: D053839:Begomovirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification* / Q000472:pathogenicity; D001938:Brazil; D016000:Cluster Analysis; D032381:Corchorus / Q000821:virology*; D004267:DNA Viruses / Q000235:genetics* / Q000302:isolation & purification; D004279:DNA, Viral / Q000737:chemistry* / Q000235:genetics*; D016679:Genome, Viral; D018551:Solanum lycopersicum / Q000821:virology; D019660:Malvaceae / Q000821:virology; D008969:Molecular Sequence Data; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D014026:Nicotiana / Q000821:virology
chemical_list: D004279:DNA, Viral