title: Deinococcus swuensis sp. nov., a gamma-radiation-resistant bacterium isolated from soil.
abstract: Strain DY59(T), a Gram-positive non-motile bacterium, was isolated from soil in South Korea, and was characterized to determine its taxonomic position. Phylogenetic analysis based on the 16S rRNA gene sequence of strain DY59(T) revealed that the strain DY59(T) belonged to the family Deinococcaceae in the class Deinococci. The highest degree of sequence similarities of strain DY59(T) were found with Deinococcus radiopugnans KACC 11999(T) (99.0%), Deinococcus marmoris KACC 12218(T) (97.9%), Deinococcus saxicola KACC 12240(T) (97.0%), Deinococcus aerolatus KACC 12745(T) (96.2%), and Deinococcus frigens KACC 12220(T) (96.1%). Chemotaxonomic data revealed that the predominant fatty acids were iso-C15:0 (19.0%), C16:1 ω7c (17.7%), C15:1 ω6c (12.6%), iso-C17:0 (10.3%), and iso-C17:1 ω9c (10.3%). A complex polar lipid profile consisted of a major unknown phosphoglycolipid. The predominant respiratory quinone is MK-8. The cell wall peptidoglycan contained D-alanine, L-glutamic acid, glycine, and L-ornithine (di-amino acid). The novel strain showed resistance to gamma radiation, with a D10 value (i.e. the dose required to reduce the bacterial population by 10-fold) in excess of 5 kGy. Based on the phylogenetic, chemotaxonomic, and phenotypic data, strain DY59(T) (=KCTC 33033(T) =JCM 18581(T)) should be classified as a type strain of a novel species, for which the name Deinococcus swuensis sp. nov. is proposed.
authors.affiliation: Department of Bio & Environmental Technology, College of Natural Science, Seoul Women's University, Seoul 139-774, Republic of Korea.
authors.forename: Jae-Jin
authors.initials: JJ
authors.lastname: Lee
authors.forename: Hyun Ji
authors.initials: HJ
authors.lastname: Lee
authors.forename: Gi Seon
authors.initials: GS
authors.lastname: Jang
authors.forename: Ja Myoung
authors.initials: JM
authors.lastname: Yu
authors.forename: Ji Yoon
authors.initials: JY
authors.lastname: Cha
authors.forename: Su Jeong
authors.initials: SJ
authors.lastname: Kim
authors.forename: Eun Bit
authors.initials: EB
authors.lastname: Lee
authors.forename: Myung Kyum
authors.initials: MK
authors.lastname: Kim
mesh_terms: D002473:Cell Wall / Q000378:metabolism / Q000528:radiation effects; D034301:Deinococcus / Q000378:metabolism* / Q000528:radiation effects; D005720:Gamma Rays; D010457:Peptidoglycan / Q000378:metabolism; D010743:Phospholipids / Q000378:metabolism; D012988:Soil Microbiology
chemical_list: D010457:Peptidoglycan; D010743:Phospholipids