title: The GoldenGate genotyping assay: custom design, processing, and data analysis.
abstract: The Illumina GoldenGate Assay is a technique that is widely used in molecular genetics to analyze up to thousands of single nucleotide polymorphism (SNPs) simultaneously, providing data of very high quality in a fast and efficient manner. This technique allows the user to optimize the number of genetic loci to be interrogated in a way that best suits their research goals. Here are described in detail all the steps to be followed in the process of genotyping a custom panel, from panel design through data analysis.
authors.affiliation: Human Genotyping Unit, Spanish National Cancer Research Center (CNIO), Madrid, Spain.
authors.forename: Anna
authors.initials: A
authors.lastname: González-Neira
mesh_terms: D016000:Cluster Analysis; D056426:Genetic Loci; D060005:Genotyping Techniques / Q000379:methods*; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*