title: Whole blood rotation thromboelastometry (ROTEM®) profiles in subjects with non-neoplastic portal vein thrombosis.
abstract: The coagulation pattern and the determinants of portal vein thrombosis (PVT), both in patients with and without cirrhosis, are still largely unknown. The aim of this study was to evaluate whole blood thromboelastometry profile, performed by ROTEM®, of both cirrhotic and non-cirrhotic subjects with PVT. Two different groups were considered: i) 14 non-cirrhotic PVT patients, ii) 35 cirrhotic patients with PVT. Controls were sex- and age-matched healthy volunteers and cirrhotic subjects without PVT, respectively. ROTEM® assays (i.e. INTEM, EXTEM, NATEM, and FIBTEM) and traditional coagulative parameters (i.e. platelet count, PT/INR, aPTT, and fibrinogen) were performed on blood samples from each subject. There were no significant differences in ROTEM® profile, as for INTEM, EXTEM, and NATEM assays, and in traditional coagulative parameters, between PVT patients, both with and without cirrhosis, and control groups. Interestingly, Maximum Clot Firmness (MCF) in FIBTEM was significantly higher in non-cirrhotic PVT patients (19 mm) than in healthy volunteers (11 mm, p<0.05). The amplitude of MCF in FIBTEM revealed to be a useful tool to discriminate non-cirrhotic subjects with PVT from those without thrombotic events. Larger prospective studies are needed to evaluate the relevance of the association between the alterations of ROTEM® profiles and PVT in cirrhotic patients.
authors.affiliation: Department of Cardiologic, Thoracic, and Vascular Sciences, 2nd Chair of Internal Medicine, University of Padua, Padua, Italy.
authors.forename: Valeria
authors.initials: V
authors.lastname: Rossetto
authors.forename: Luca
authors.initials: L
authors.lastname: Spiezia
authors.forename: Marco
authors.initials: M
authors.lastname: Senzolo
authors.forename: Kryssia I
authors.initials: KI
authors.lastname: Rodriguez-Castro
authors.forename: Sara
authors.initials: S
authors.lastname: Maggiolo
authors.forename: Paolo
authors.initials: P
authors.lastname: Simioni
mesh_terms: D000328:Adult; D000368:Aged; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D011169:Portal Vein; D013916:Thrombelastography / Q000379:methods*; D020246:Venous Thrombosis / Q000097:blood*; D055815:Young Adult
keywords: CFT; CT; Cirrhosis; Clot Formation Time; Clotting Time; INR; International Normalized Ratio; MCF; Maximum Clot Firmness; PT; PVT; Portal Vein Thrombosis; Portal vein thrombosis; Prothrombin Time; ROC; Receiver Operating Characteristic; Thromboelastometry; WB; Whole Blood; aPTT; activated Partial Thromboplastin Time