title: Cytotoxic activity and apoptosis induction by gaillardin.
abstract: Cytotoxic activity of gaillardin, a sesquiterpene lactone isolated from Inula oculus-christi L. (Asteraceae), was assessed in the human breast adenocarcinoma cell line MCF-7, human hepatocellular carcinoma cell line HepG-2, human non-small cell lung carcinoma cell line A-549, and human colon adenocarcinoma cell line HT-29, resulting in IC50 values of 6.37, 6.20, 4.76, and 1.81 microg/mL, respectively, in the microculture tetrazolium-formazan MTT assay. In vitro apoptosis-inducing properties of gaillardin were also evaluated in MCF-7 cells with the terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick-end labeling (TUNEL) assay. The results suggest gaillardin as a candidate for further studies in cancer therapy
authors.affiliation: Department of Traditional Pharmacy, School of Traditional Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors.forename: Maryam Hamzeloo
authors.initials: MH
authors.lastname: Moghadam
authors.forename: Farzaneh
authors.initials: F
authors.lastname: Naghibi
authors.forename: Azadeh
authors.initials: A
authors.lastname: Atoofi
authors.forename: Mitra Asgharian
authors.initials: MA
authors.lastname: Rezaie
authors.forename: Mahboobeh
authors.initials: M
authors.lastname: Irani
authors.forename: Mahmoud
authors.initials: M
authors.lastname: Mosaddegh
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D007783:Lactones / Q000494:pharmacology; D012717:Sesquiterpenes / Q000494:pharmacology
chemical_list: D007783:Lactones; D012717:Sesquiterpenes; C476872:gaillardin