title: Lysinibacillus chungkukjangi sp. nov., isolated from Chungkukjang, Korean fermented soybean food.
abstract: One bacterial strain 2RL3-2(T) was isolated from Chungkukjang, a traditional Korean fermented food made from soybeans, and determined to be a Gram-positive, aerobic, spore-forming rod. Growth of the novel strain was optimal at 30°C and pH 7.0. The 16S rRNA gene of strain 2RL3-2(T) showed the highest level of sequence similarity to Lysinibacillus sinduriensis BLB-1(T) (99.0%), Lysinibacillus massiliensis 4400831(T) (97.1%), Lysinibacillus xylanilyticus XDB9(T) (97.0%), and Lysinibacillus odysseyi 34hs-1(T) (96.8%). Phylogenetic analysis showed that strain 2RL3-2(T) formed a robust cluster with L. sinduriensis BLB-1(T), L. massiliensis 4400831(T), and L. odyssey 34hs-1(T). The major fatty acids were anteiso-C15:0 (47.3%), iso-C16:0 (16.3%), and anteiso-C17:0 (11.3%), and the only menaquinone was MK-7. Diphosphatidylglycerol, phosphatidylglycerol, and phosphatidylethanolamine were the major polar lipids, along with an unknown phospholipid and two unknown lipids. The peptidoglycan type was A4α, with an interpeptide bridge of l-Lys-d-Asp. DNA-DNA hybridization values between strain 2RL3-2(T) and closely related Lysinibacillus species were below 43±4%. Therefore, based on phenotypic, chemotaxonomic, and phylogenetic characteristics, it was determined that strain 2RL3-2(T) represents a novel species of the genus Lysinibacillus, for which the name Lysinibacillus chungkukjangi sp. nov. is proposed. The type strain is 2RL3-2(T) (=KACC 16626(T) =NBRC 108948(T)).
authors.affiliation: Agricultural Microbiology Division, National Academy of Agricultural Science, Rural Development Administration, Suwon 441-707, Republic of Korea.
authors.forename: Soo-Jin
authors.initials: SJ
authors.lastname: Kim
authors.forename: Yun-Hee
authors.initials: YH
authors.lastname: Jang
authors.forename: Moriyuki
authors.initials: M
authors.lastname: Hamada
authors.forename: Jae-Hyung
authors.initials: JH
authors.lastname: Ahn
authors.forename: Hang-Yeon
authors.initials: HY
authors.lastname: Weon
authors.forename: Ken-ichiro
authors.initials: K
authors.lastname: Suzuki
authors.forename: Kyung-Sook
authors.initials: KS
authors.lastname: Whang
authors.forename: Soon-Wo
authors.initials: SW
authors.lastname: Kwon
mesh_terms: D001406:Bacillaceae / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D002308:Cardiolipins / Q000378:metabolism; D005285:Fermentation; D010714:Phosphatidylethanolamines / Q000378:metabolism; D010715:Phosphatidylglycerols / Q000378:metabolism; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013025:Glycine max
chemical_list: D002308:Cardiolipins; D010714:Phosphatidylethanolamines; D010715:Phosphatidylglycerols; D012336:RNA, Ribosomal, 16S; C483858:phosphatidylethanolamine