title: miR-19a and miR-19b overexpression in gliomas.
abstract: Astrocytic gliomas are the most common type of human primary brain tumors with poor prognosis. MicroRNAs(miRs) are frequently deregulated in gliomas and play an oncogenic or tumor suppressor role. In our previous study we found that miR-19a and miR-19b were up-regulated in malignant glioma cell lines by microRNA array. For further validation of this finding, the expression of miR-19a and miR-19b was detected by qRT-PCR and in situ hybridization(ISH) in 8 malignant glioma cell lines, 43 freshly resected glioma samples and 75 archival paraffin embedded glioma specimens with different grades of malignancy in the present study. The results demonstrate that miR-19a and miR-19b are overexpressed in glioma cell lines and astrocytic glioma tissues, and their expression level is positively correlated with tumor grades. Additionally, the tumor suppressor gene PTEN is identified as the target of miR-19a and miR-19b by Luciferase assay. It is speculated that miR-19a and miR-19b may have an oncogenic role in gliomagenesis at least partially via the negative regulation of PTEN and the molecular mechanism of gliomagenesis in which miR 19a and miR-19b involved should be investigated further.
authors.affiliation: Department of Neurosurgery, Tianjin Neurological Institute, Laboratory of Neuro-Oncology, Key Laboratory of Post-trauma Neuro-repair and Regeneration in Central Nervous System, Ministry of Education, Tianjin Key Laboratory of Injuries, Variations and Regeneration of Nervous System, Tianjin Medical University General Hospital, 152 An-Shan Road, Tianjin, 300052, People's Republic of China.
authors.forename: Zhifan
authors.initials: Z
authors.lastname: Jia
authors.forename: Kun
authors.initials: K
authors.lastname: Wang
authors.forename: Anling
authors.initials: A
authors.lastname: Zhang
authors.forename: Guangxiu
authors.initials: G
authors.lastname: Wang
authors.forename: Chunsheng
authors.initials: C
authors.lastname: Kang
authors.forename: Lei
authors.initials: L
authors.lastname: Han
authors.forename: Peiyu
authors.initials: P
authors.lastname: Pu
mesh_terms: D000704:Analysis of Variance; D045744:Cell Line, Tumor; D005910:Glioma / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D017403:In Situ Hybridization; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics; D051059:PTEN Phosphohydrolase / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction
chemical_list: C549214:MIRN19A microRNA, human; D035683:MicroRNAs; D051059:PTEN Phosphohydrolase; C494929:PTEN protein, human