title: Antigen-bearing dendritic cells from the sublingual mucosa recirculate to distant systemic lymphoid organs to prime mucosal CD8 T cells.
abstract: Effector T cells are described to be primed in the lymph nodes draining the site of immunization and to recirculate to effector sites. Sublingual immunization generates effector T cells able to disseminate to the genital tract. Herein, we report an alternative mechanism that involves the recirculation of antigen-bearing dendritic cells (DCs) in remote lymphoid organs to prime T cells. Sublingual immunization with a muco-adhesive model antigen unable to diffuse through lymphatic or blood vessels induced genital CD8 T cells. The sublingual draining lymph nodes were not mandatory to generate these lymphocytes, and antigen-bearing DCs from distant lymph nodes and spleen were able to prime specific CD8 T cells in a time- and dose-dependent manner. This study demonstrates, for the first time, that antigen-bearing DCs originating from the site of immunization recirculate to distant lymphoid organs and provides insights into the mechanism of distant CD8 T-cell generation by sublingual immunization.
authors.affiliation: 1] INSERM, UMR 634, Faculté de Médecine Pasteur, Nice, France [2] University of Nice-Sophia Antipolis, Nice, France [3] CNRS, CNRS/UNSA UMR7275, Institut de Pharmacologie Moléculaire et Cellulaire, Valbonne, France.
authors.forename: C
authors.initials: C
authors.lastname: Hervouet
authors.affiliation: 1] INSERM, UMR 634, Faculté de Médecine Pasteur, Nice, France [2] University of Nice-Sophia Antipolis, Nice, France [3] CNRS, CNRS/UNSA UMR7275, Institut de Pharmacologie Moléculaire et Cellulaire, Valbonne, France.
authors.forename: C
authors.initials: C
authors.lastname: Luci
authors.affiliation: 1] INSERM, UMR 634, Faculté de Médecine Pasteur, Nice, France [2] University of Nice-Sophia Antipolis, Nice, France [3] CNRS, CNRS/UNSA UMR7275, Institut de Pharmacologie Moléculaire et Cellulaire, Valbonne, France.
authors.forename: S
authors.initials: S
authors.lastname: Bekri
authors.affiliation: 1] INSERM, UMR 634, Faculté de Médecine Pasteur, Nice, France [2] University of Nice-Sophia Antipolis, Nice, France.
authors.forename: T
authors.initials: T
authors.lastname: Juhel
authors.affiliation: 1] University of Nice-Sophia Antipolis, Nice, France [2] CNRS, CNRS/UNSA UMR7275, Institut de Pharmacologie Moléculaire et Cellulaire, Valbonne, France.
authors.forename: F
authors.initials: F
authors.lastname: Bihl
authors.affiliation: 1] University of Nice-Sophia Antipolis, Nice, France [2] CNRS, CNRS/UNSA UMR7275, Institut de Pharmacologie Moléculaire et Cellulaire, Valbonne, France.
authors.forename: V M
authors.initials: VM
authors.lastname: Braud
authors.affiliation: International Vaccine Institute, Seoul, Korea.
authors.forename: C
authors.initials: C
authors.lastname: Czerkinsky
authors.affiliation: 1] INSERM, UMR 634, Faculté de Médecine Pasteur, Nice, France [2] University of Nice-Sophia Antipolis, Nice, France [3] CNRS, CNRS/UNSA UMR7275, Institut de Pharmacologie Moléculaire et Cellulaire, Valbonne, France.
authors.forename: F
authors.initials: F
authors.lastname: Anjuère
mesh_terms: D000818:Animals; D000941:Antigens / Q000276:immunology*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D002450:Cell Communication / Q000276:immunology; D002465:Cell Movement / Q000276:immunology; D003713:Dendritic Cells / Q000276:immunology* / Q000378:metabolism; D008198:Lymph Nodes / Q000276:immunology; D008221:Lymphoid Tissue / Q000276:immunology*; D051379:Mice; D009060:Mouth Floor; D009061:Mouth Mucosa / Q000276:immunology*; D013997:Time Factors
chemical_list: D000941:Antigens