title: Fermented Prunus mume with probiotics inhibits 7,12-dimethylbenz[a]anthracene and 12-o-tetradecanoyl phorbol-13-acetate induced skin carcinogenesis through alleviation of oxidative stress.
abstract: Maesil (Prunus mume Siebold and Zucc.), a member of the genus Rosaceae, has been reported to have antioxidative effects, as well as anticancer influence in many cancer lines. Thus, this present study was designed to investigate the inhibitory effect of fermented Maesil with probiotics against 7,12-dimethylbenz[a]anthracene (DMBA), 12-O-tetradecanoyl phorbol 13-acetate (TPA)-induced mouse skin carcinogenesis via its antioxidative potential. Mice were fed a diet containing fermented Maesil, containing either 1% (1% FM fed group) or 2% (2% FM fed group) along with probiotics following DMBA and TPA exposure. Continuous ingestion of the experimental feed markedly inhibited skin carcinogenesis, as evidenced by a marked decrease in papilloma numbers and epidermal hyperplasia as well as cellular proliferation and the percentage of proliferating-cell nuclear antigen positive cells. Also, the FM fed group showed an increase of total antioxidant capacity as well as an increased level of phase II detoxifying enzymes such as superoxide dismutase, concurrent with a decreased lipid peroxidation activity level. Taken together, these results suggest that fermented Maesil has the ability to suppress the development of DMBA-TPA induced skin carcinogenesis, via the reduction of lipid peroxidation, enhancing total antioxidant capacity and phase II detoxifying enzyme.
authors.affiliation: Department of Veterinary Infectious Disease, College of Veterinary Medicine, Chonnam National University, Gwangju, Korea.
authors.forename: Jin-A
authors.initials: JA
authors.lastname: Lee
authors.forename: Jae-Hyung
authors.initials: JH
authors.lastname: Ko
authors.forename: Bock-Gie
authors.initials: BG
authors.lastname: Jung
authors.forename: Tae-Hoon
authors.initials: TH
authors.lastname: Kim
authors.forename: Ji-In
authors.initials: JI
authors.lastname: Hong
authors.forename: Young-Seok
authors.initials: YS
authors.lastname: Park
authors.forename: Bong-Joo
authors.initials: BJ
authors.lastname: Lee
mesh_terms: D015127:9,10-Dimethyl-1,2-benzanthracene; D000818:Animals; D000970:Antineoplastic Agents / Q000627:therapeutic use; D000975:Antioxidants / Q000627:therapeutic use; D049109:Cell Proliferation / Q000187:drug effects; D002471:Cell Transformation, Neoplastic / Q000139:chemically induced / Q000187:drug effects*; D004032:Diet; D005260:Female; D005285:Fermentation; D015227:Lipid Peroxidation / Q000187:drug effects; D051379:Mice; D008807:Mice, Inbred BALB C; D018384:Oxidative Stress / Q000187:drug effects*; D028321:Plant Preparations / Q000627:therapeutic use*; D019936:Probiotics / Q000627:therapeutic use; D027861:Prunus / Q000378:metabolism*; D012867:Skin / Q000473:pathology; D012878:Skin Neoplasms / Q000139:chemically induced / Q000188:drug therapy* / Q000517:prevention & control; D013482:Superoxide Dismutase / Q000096:biosynthesis / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate
chemical_list: D000970:Antineoplastic Agents; D000975:Antioxidants; D028321:Plant Preparations; D015127:9,10-Dimethyl-1,2-benzanthracene; D013482:Superoxide Dismutase; D013755:Tetradecanoylphorbol Acetate