title: Accumulated phosphatidylcholine (16:0/16:1) in human colorectal cancer; possible involvement of LPCAT4.
abstract: The identification of cancer biomarkers is critical for target-linked cancer therapy. The overall level of phosphatidylcholine (PC) is elevated in colorectal cancer (CRC). To investigate which species of PC is overexpressed in colorectal cancer, an imaging mass spectrometry was performed using a panel of non-neoplastic mucosal and CRC tissues. In the present study, we identified a novel biomarker, PC(16:0/16:1), in CRC using imaging mass spectrometry. Specifically, elevated levels of PC(16:0/16:1) expression were observed in the more advanced stage of CRC. Our data further showed that PC(16:0/16:1) was specifically localized in the cancer region when examined using imaging mass spectrometry. Notably, because the ratio of PC(16:0/16:1) to lyso-PC(16:0) was higher in CRC, we postulated that lyso-PC acyltransferase (LPCAT) activity is elevated in CRC. In an in vitro analysis, we showed that LPCAT4 is involved in the deregulation of PC(16:0/16:1) in CRC. In an immunohistochemical analysis, LPCAT4 was shown to be overexpressed in CRC. These data indicate the potential usefulness of PC(16:0/16:1) for the clinical diagnosis of CRC and implicate LPCAT4 in the elevated expression of PC(16:0/16:1) in CRC.
authors.affiliation: Department of Tumor Pathology, Hamamatsu University School of Medicine, Shizuoka, Japan.
authors.forename: Nobuya
authors.initials: N
authors.lastname: Kurabe
authors.forename: Takahiro
authors.initials: T
authors.lastname: Hayasaka
authors.forename: Mikako
authors.initials: M
authors.lastname: Ogawa
authors.forename: Noritaka
authors.initials: N
authors.lastname: Masaki
authors.forename: Yoshimi
authors.initials: Y
authors.lastname: Ide
authors.forename: Michihiko
authors.initials: M
authors.lastname: Waki
authors.forename: Toshio
authors.initials: T
authors.lastname: Nakamura
authors.forename: Kiyotaka
authors.initials: K
authors.lastname: Kurachi
authors.forename: Tomoaki
authors.initials: T
authors.lastname: Kahyo
authors.forename: Kazuya
authors.initials: K
authors.lastname: Shinmura
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Midorikawa
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Sugiyama
authors.forename: Mitsutoshi
authors.initials: M
authors.lastname: Setou
authors.forename: Haruhiko
authors.initials: H
authors.lastname: Sugimura
mesh_terms: D051103:1-Acylglycerol-3-Phosphate O-Acyltransferase / Q000032:analysis* / Q000235:genetics / Q000502:physiology; D008243:1-Acylglycerophosphocholine O-Acyltransferase; D000230:Adenocarcinoma / Q000737:chemistry* / Q000175:diagnosis / Q000201:enzymology; D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor; D045744:Cell Line, Tumor; D015179:Colorectal Neoplasms / Q000737:chemistry* / Q000175:diagnosis / Q000201:enzymology; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007413:Intestinal Mucosa / Q000737:chemistry; D008297:Male; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000032:analysis* / Q000235:genetics / Q000502:physiology; D010713:Phosphatidylcholines / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D014408:Biomarkers, Tumor; D009363:Neoplasm Proteins; D010713:Phosphatidylcholines; D008243:1-Acylglycerophosphocholine O-Acyltransferase; C508935:LPCAT4 protein, human; D051103:1-Acylglycerol-3-Phosphate O-Acyltransferase