title: Afrormosin, an Isoflavonoid from Amburana cearensis A. C. Smith, Modulates the Inflammatory Response of Stimulated Human Neutrophils.
abstract: Isoflavones are phytoestrogens known by their anti-inflammatory, antioxidant and immunomodulatory properties. Presently, there is no information on whether afrormosin, an isoflavone from Amburana cearensis A.C. Smith (Fabaceae), has some effect on the inflammatory response from stimulated human neutrophils. Thus, the aim of this study was to evaluate the anti-inflammatory and antioxidant potentials of afrormosin on human neutrophils. Neutrophils (2.5 × 10(6) cells/mL) were incubated with afrormosin (3.35-335.2 μM) prepared from a product isolated from Amburana cearensis A.C. Smith with a 78.5% degree of purity and stimulated by the addition of cytochalasin B and N-formyl-methionyl-leucyl-phenylalanine (fMLP) or phorbol 12-myristate-13-acetate (PMA). Afrormosin inhibited the neutrophil degranulation induced by fMLP (10.47-335.2 μM) or PMA (0.33-167.6 μM), myeloperoxidase activity (3.3-335.2 μM), TNF-α secretion (16.7-335.2 μM) and the reactive oxygen species (ROS) generation (16.7-335.2 μM). On the other hand, afrormosin did not show any effect either on elastase or as a free radical scavenger. These data suggest that afrormosin modulates intermediary steps of the neutrophil ROS generation process. In addition, the modulatory effect of afrormosin on human neutrophil degranulation seems to be directed towards PMA-induced activation, indicating a potent inhibition of the protein kinase C activity. This study provided evidence, for the first time, to support the anti-inflammatory and antioxidant activities of afrormosin, creating novel insights into the pharmacological actions of this natural isoflavone.
authors.affiliation: Center for Pharmaceutical and Cosmetic Studies, Department of Pharmacy, Faculty of Pharmacy, Odontology and Nursing, Federal University of Ceará (UFC), Fortaleza, Brazil.
authors.forename: Amanda
authors.initials: A
authors.lastname: de Araújo Lopes
authors.affiliation: Center for Pharmaceutical and Cosmetic Studies, Department of Pharmacy, Faculty of Pharmacy, Odontology and Nursing, Federal University of Ceará (UFC), Fortaleza, Brazil.
authors.forename: Talita R
authors.initials: TR
authors.lastname: Magalhães
authors.affiliation: Department of Organic and Inorganic Chemistry, Federal University of Ceará (UFC), Fortaleza, Brazil.
authors.forename: Daniel E
authors.initials: DE
authors.lastname: de Andrade Uchôa
authors.affiliation: Department of Organic and Inorganic Chemistry, Federal University of Ceará (UFC), Fortaleza, Brazil.
authors.forename: Edilberto R
authors.initials: ER
authors.lastname: Silveira
authors.affiliation: Department of Physics and Chemistry, University of São Paulo (USP), São Paulo, Brazil.
authors.forename: Ana E C S
authors.initials: AE
authors.lastname: Azzolini
authors.affiliation: Department of Physics and Chemistry, University of São Paulo (USP), São Paulo, Brazil.
authors.forename: Luciana M
authors.initials: LM
authors.lastname: Kabeya
authors.affiliation: Department of Physics and Chemistry, University of São Paulo (USP), São Paulo, Brazil.
authors.forename: Yara M
authors.initials: YM
authors.lastname: Lucisano-Valim
authors.affiliation: Department of Physiology and Pharmacology, Federal University of Ceará (UFC), Fortaleza, Brazil.
authors.forename: Silvânia M M
authors.initials: SM
authors.lastname: Vasconcelos
authors.affiliation: Department of Physiology and Pharmacology, Federal University of Ceará (UFC), Fortaleza, Brazil.
authors.forename: Glauce S
authors.initials: GS
authors.lastname: de Barros Viana
authors.affiliation: Center for Pharmaceutical and Cosmetic Studies, Department of Pharmacy, Faculty of Pharmacy, Odontology and Nursing, Federal University of Ceará (UFC), Fortaleza, Brazil.
authors.forename: Luzia K A M
authors.initials: LK
authors.lastname: Leal
mesh_terms: D000328:Adult; D000975:Antioxidants / Q000494:pharmacology; D015550:Cell Degranulation / Q000187:drug effects; D007887:Fabaceae / Q000737:chemistry*; D006801:Humans; D007249:Inflammation / Q000188:drug therapy*; D018836:Inflammation Mediators / Q000494:pharmacology*; D007529:Isoflavones / Q000302:isolation & purification / Q000494:pharmacology*; D009504:Neutrophils / Q000737:chemistry / Q000187:drug effects*; D010196:Pancreatic Elastase / Q000187:drug effects; D009195:Peroxidase / Q000187:drug effects; D017382:Reactive Oxygen Species / Q000032:analysis; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis
chemical_list: C000602523:7-hydroxy-6,4'-dimethoxyisoflavone; D000975:Antioxidants; D018836:Inflammation Mediators; D007529:Isoflavones; D017382:Reactive Oxygen Species; D014409:Tumor Necrosis Factor-alpha; D009195:Peroxidase; D010196:Pancreatic Elastase