title: Preliminary results on antigenotoxic effects of dried mycelia of two medicinal mushrooms in Drosophila melanogaster somatic mutation and recombination test.
abstract: In this study, the antigenotoxic effects of dried mycelia from Trametes versicolor and Pleurotus ostreatus were investigated using a Drosophila melanogaster somatic mutation and recombination test (SMART), which is based on the principle that the loss of heterozygosity of suitable recessive marker hairs, such as multiple wing (mwh) and flare-3 (flr3), can lead to the formation of mutant clones of larval cells, which then are expressed as spots on the wings of the adult flies. In this study, dried mycelia from T. versicolor and P. ostreatus alone (7.5, 15, and 30 mg) were examined for genotoxicity and combined with mitomycin C (MMC; 0.05 mM) for antigenotoxicity. Commonly known mutagen which is mitomycin C (MMC) was used as positive control. The results showed that the dried mycelia of mushrooms were not genotoxic themselves. Nevertheless, the mushrooms have antigenotoxic activity by reducing the frequency of MMC-induced spots in varying proportions. Thus, powders of these 2 fungi were able to suppress somatic cell mutation induced by MMC. These results suggest that T. versicolor and P. ostreatus have antigenotoxic activity, including antirecombinogenic activity.
authors.affiliation: Department of Biology, Inonu University, Malatya, Turkey.
authors.forename: Aygul
authors.initials: A
authors.lastname: Kylyc
authors.forename: Elif
authors.initials: E
authors.lastname: Yesilada
mesh_terms: D000818:Animals; D004249:DNA Damage; D004331:Drosophila melanogaster; D009152:Mutagenicity Tests / Q000379:methods*; D009153:Mutagens; D025282:Mycelium / Q000737:chemistry*; D020076:Pleurotus / Q000737:chemistry*; D055454:Trametes / Q000737:chemistry*
chemical_list: D009153:Mutagens