title: Changes in expression of BetV1 allergen of silver birch pollen in urbanized area of Ukraine.
abstract: The aim of the study was to determinate the level of expression of silver birch allergen Betv1 in pollen samples from different Ukraine areas by RT-qPCR SYBR Green assay. Protocol for quantifying the expression of Betv1 allergen was developed when testing of three housekeeping genes-cyclophylin, alpha-tubulin and transcription factor CBF1. Samples from urbanized area was analysed by real-time PCR when a sample from forest growth conditions was used as a calibrator. Real-time PCR based quantifying of Betv1 provides a useful method for rapid and sensitive analyses of this silver birch allergen. Our results show higher expression levels in samples from central parts of urbanized area as housing estates when compared to the samples from borders of the urbanized area.
authors.affiliation: Department of Genetics and Plant Breeding, Slovak University of Agriculture in Nitra, Nitra, Slovak Republic. jana.ziarovska@uniag.sk
authors.forename: Jana
authors.initials: J
authors.lastname: Žiarovská
authors.forename: Mária
authors.initials: M
authors.lastname: Labajová
authors.forename: Katarína
authors.initials: K
authors.lastname: Ražná
authors.forename: Milan
authors.initials: M
authors.lastname: Bežo
authors.forename: Veronika
authors.initials: V
authors.lastname: Štefúnová
authors.forename: Tatiana
authors.initials: T
authors.lastname: Shevtsova
authors.forename: Kateryna
authors.initials: K
authors.lastname: Garkava
authors.forename: Ján
authors.initials: J
authors.lastname: Brindza
mesh_terms: D000485:Allergens / Q000235:genetics*; D052179:Antigens, Plant / Q000235:genetics*; D029662:Betula / Q000235:genetics*; D002947:Cities; D015870:Gene Expression; D011058:Pollen; D060888:Real-Time Polymerase Chain Reaction; D014455:Ukraine
chemical_list: D000485:Allergens; D052179:Antigens, Plant; C061312:Bet v 1 allergen, Betula