title: From genes to function: the C. elegans genetic toolbox.
abstract: This review aims to provide an overview of the technologies which make the nematode Caenorhabditis elegans an attractive genetic model system. We describe transgenesis techniques and forward and reverse genetic approaches to isolate mutants and clone genes. In addition, we discuss the new possibilities offered by genome engineering strategies and next-generation genome analysis tools.
authors.affiliation: Department of Biology, Institut de Biologie de l'École Normale Supérieure, Paris, France. boulin@biologie.ens.fr
authors.forename: Thomas
authors.initials: T
authors.lastname: Boulin
authors.forename: Oliver
authors.initials: O
authors.lastname: Hobert
mesh_terms: D000818:Animals; D017173:Caenorhabditis elegans / Q000235:genetics*; D002874:Chromosome Mapping; D055028:Comparative Genomic Hybridization; D017238:Genes, Helminth; D016296:Mutagenesis; D020641:Polymorphism, Single Nucleotide; D034622:RNA Interference