title: Inhibitory effect of components from Streptomyces species on alpha-glucosidase and alpha-amilase of different origin.
abstract: The search for the effective and safe a-glucosidase and alpha-amylase inhibitors from Actinomycetaceae being antidiabetic agents is actual problem. Twenty one Streptomyces spp. of soil samples collected from different places of China were screened for the ability to produce this kind of inhibitory activities. Fermentation broth of isolated strains had absorbance between 350-190 nm. The Streptomyces strains PW003, ZG636, and ZG731 were characterized by special absorption at 280, 275, and 400 nm, respectively. Ten of the collected actinomycete strains had the ability to inhibit alpha-glucosidase or/and alpha-amylase and the fermentation broth of the same strain had inhibitory activity varied greatly depending on the enzyme source. In the process to screen the leading compounds used as antidiabetic agents, human alpha-glucosidase and alpha-amylase were revealed as the best used in trail compared with the same enzymes from other sources. Active alpha-glucosidase inhibitor was isolated from Streptomyces strain PW638 fermentation broth and identified as acarviostatin 103 by MS and N MR spectrometry. Its IC50 value was 1.25 and 12.23 microg/mL against human intestinal N-terminal maltase-glucoamylase and human pancreatic alpha-amylase, respectively.
authors.affiliation: Nankai University, Tianjin 300071, China.
authors.forename: P
authors.initials: P
authors.lastname: Meng
authors.forename: C
authors.initials: C
authors.lastname: Xie
authors.forename: P
authors.initials: P
authors.lastname: Geng
authors.forename: X
authors.initials: X
authors.lastname: Qi
authors.forename: F
authors.initials: F
authors.lastname: Zheng
authors.forename: F
authors.initials: F
authors.lastname: Bai
mesh_terms: D000818:Animals; D002681:China; D057075:Enzyme Assays; D004791:Enzyme Inhibitors / Q000737:chemistry* / Q000302:isolation & purification; D005285:Fermentation; D065089:Glycoside Hydrolase Inhibitors; D006801:Humans; D007004:Hypoglycemic Agents / Q000737:chemistry* / Q000302:isolation & purification; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008855:Microscopy, Electron, Scanning; D009844:Oligosaccharides / Q000737:chemistry* / Q000302:isolation & purification; D055292:Pancreatic alpha-Amylases / Q000037:antagonists & inhibitors*; D051381:Rats; D012988:Soil Microbiology; D013302:Streptomyces / Q000737:chemistry* / Q000378:metabolism / Q000648:ultrastructure
chemical_list: D004791:Enzyme Inhibitors; D065089:Glycoside Hydrolase Inhibitors; D007004:Hypoglycemic Agents; D009844:Oligosaccharides; D055292:Pancreatic alpha-Amylases