title: Biosynthetic machinery of ionophore polyether lasalocid: enzymatic construction of polyether skeleton.
abstract: Diversity of natural polycyclic polyethers originated from very simple yet versatile strategy consisting of epoxidation of linear polyene followed by epoxide opening cascade. To understand two-step enzymatic transformations at molecular basis, a flavin containing monooxygenase (EPX) Lsd18 and an epoxide hydrolase (EH) Lsd19 were selected as model enzymes for extensive investigation on substrate specificity, catalytic mechanism, cofactor requirement and crystal structure. This pioneering study on prototypical lasalocid EPX and EH provides insight into detailed mechanism of ionophore polyether assembly machinery and clarified remaining issues for polyether biosynthesis.
authors.affiliation: Division of Chemistry, Graduate School of Science, Hokkaido University, Sapporo 060-0810, Japan.
authors.forename: Atsushi
authors.initials: A
authors.lastname: Minami
authors.forename: Hiroki
authors.initials: H
authors.lastname: Oguri
authors.forename: Kenji
authors.initials: K
authors.lastname: Watanabe
authors.forename: Hideaki
authors.initials: H
authors.lastname: Oikawa
mesh_terms: D004852:Epoxy Compounds / Q000737:chemistry; D004987:Ethers / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D007476:Ionophores / Q000737:chemistry / Q000378:metabolism*; D007832:Lasalocid / Q000096:biosynthesis* / Q000737:chemistry; D013237:Stereoisomerism
chemical_list: D004852:Epoxy Compounds; D004987:Ethers; D007476:Ionophores; D007832:Lasalocid