title: Growth of xenotransplanted leukemia cells is influenced by diet nutrients and is attenuated with 2-deoxyglucose.
abstract: We examined the effects of diet nutrients on xenotransplanted leukemia cells, THP-1 or NB4. THP-1 tumors showed more growth when fed with high fat diet, while NB4 tumors grew more with high carbohydrate diet. Then, administration of 2-deoxyglucose (a glycolysis inhibitor) showed a significant antitumor effect on both tumors: NB4 tumor showed large necrotic areas, while THP-1 tumor did not, but had augmented expression of enzymes for fatty acid oxidation. 2-Deoxyglucose inhibited the growth of NB4 by cell death because main energy producing pathway (glycolysis) was abolished, while 2-deoxyglucose slowed the growth of THP-1 by shifting energy metabolism to fatty acid β-oxidation.
authors.affiliation: Department of Internal Medicine, Division of Hematology, Aichi Medical University School of Medicine, Nagakute, Aichi 480-1195, Japan.
authors.forename: Norikazu
authors.initials: N
authors.lastname: Tsunekawa-Imai
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Miwa
authors.forename: Masato
authors.initials: M
authors.lastname: Shikami
authors.forename: Kazuto
authors.initials: K
authors.lastname: Suganuma
authors.forename: Mineaki
authors.initials: M
authors.lastname: Goto
authors.forename: Shohei
authors.initials: S
authors.lastname: Mizuno
authors.forename: Miyuki
authors.initials: M
authors.lastname: Takahashi
authors.forename: Motonori
authors.initials: M
authors.lastname: Mizutani
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Horio
authors.forename: Hiroko
authors.initials: H
authors.lastname: Komatsubara
authors.forename: Mayuko
authors.initials: M
authors.lastname: Gotou
authors.forename: Hidesuke
authors.initials: H
authors.lastname: Yamamoto
authors.forename: Motohiro
authors.initials: M
authors.lastname: Wakabayashi
authors.forename: Masaya
authors.initials: M
authors.lastname: Watarai
authors.forename: Ichiro
authors.initials: I
authors.lastname: Hanamura
authors.forename: Akira
authors.initials: A
authors.lastname: Imamura
authors.forename: Hidetsugu
authors.initials: H
authors.lastname: Mihara
authors.forename: Masakazu
authors.initials: M
authors.lastname: Nitta
mesh_terms: D000818:Animals; D000963:Antimetabolites / Q000494:pharmacology*; D015153:Blotting, Western; D049109:Cell Proliferation; D003847:Deoxyglucose / Q000494:pharmacology*; D004032:Diet; D019587:Dietary Supplements; D004734:Energy Metabolism / Q000187:drug effects; D005260:Female; D006019:Glycolysis / Q000187:drug effects; D006801:Humans; D007124:Immunoenzyme Techniques; D007942:Leukemia, Experimental / Q000188:drug therapy* / Q000378:metabolism; D051379:Mice; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D009368:Neoplasm Transplantation; D014183:Transplantation, Heterologous; D014407:Tumor Cells, Cultured
chemical_list: D000963:Antimetabolites; D003847:Deoxyglucose
keywords: 2-Deoxyglucose (2-DG); AML; Carbohydrate-dominant diet; Etomoxir; High fat diet; Xenotransplantation