title: Pharmacological study of cholinergic system on cardiovascular regulation in the cuneiform nucleus of rat.
abstract: In the present study the effect of cholinergic system of Cuneiform nucleus (CnF) on central regulation of cardiovascular system was investigated. Two doses of acetylcholine (Ach; 90 and 150 nmol), atropine (3 and 9 nmol) and hexamethonium (Hexa; 100 and 300 nmol) were microinjected into the CnF. The maximum changes of MAP and HR were compared with control group (independent t-test). Both doses of Ach significantly decreased MAP but had no significant effect on HR. Administration of atropine and Hexa by themselves did not alter the MAP or HR. However, both doses of atropine and higher dose of Hexa significantly attenuated the hypotensive effect of Ach with no significant effect on HR. Our results suggest the involvement of CnF cholinergic system only on central blood pressure regulation that strongly mediated by muscarinic receptors.
authors.affiliation: Neurocognitive Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran. Shafeimn@mums.ac.ir
authors.forename: Mohammad Naser
authors.initials: MN
authors.lastname: Shafei
authors.forename: Saeed
authors.initials: S
authors.lastname: Niazmand
authors.forename: Lili
authors.initials: L
authors.lastname: Enayatfard
authors.forename: Mahmoud
authors.initials: M
authors.lastname: Hosseini
authors.forename: Mahdi Hasanzade
authors.initials: MH
authors.lastname: Daloee
mesh_terms: D000109:Acetylcholine / Q000008:administration & dosage*; D000818:Animals; D001285:Atropine / Q000008:administration & dosage*; D001794:Blood Pressure / Q000187:drug effects / Q000502:physiology; D002319:Cardiovascular System / Q000187:drug effects* / Q000378:metabolism; D018679:Cholinergic Agonists / Q000008:administration & dosage*; D006339:Heart Rate / Q000187:drug effects / Q000502:physiology; D008297:Male; D008636:Mesencephalon / Q000187:drug effects* / Q000378:metabolism; D008845:Microinjections; D018727:Muscarinic Antagonists / Q000008:administration & dosage*; D051381:Rats; D017208:Rats, Wistar; D013681:Tegmentum Mesencephali / Q000187:drug effects* / Q000378:metabolism
chemical_list: D018679:Cholinergic Agonists; D018727:Muscarinic Antagonists; D001285:Atropine; D000109:Acetylcholine
keywords: Acetylcholine; Atropine; Cuneiform nucleus; Hexametunium; MAP; Microinjection