title: Carbon nanodots as a matrix for the analysis of low-molecular-weight molecules in both positive- and negative-ion matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and quantification of glucose and uric acid in real samples.
abstract: Carbon nanodots were applied for the first time as a new matrix for the analysis of low-molecular-weight compounds by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) in both positive- and negative-ion modes. A wide range of small molecules including amino acids, peptides, fatty acids, as well as β-agonists and neutral oligosaccharides were analyzed by MALDI MS with carbon nanodots as the matrix, and the lowest 0.2 fmol limits-of-detection were obtained for octadecanoic acid. Clear sodium and potassium adducts and deprotonated signals were produced in positive- and negative-ion modes. Furthermore, the glucose and uric acid in real samples were quantitatively determined by the internal standard method with the linear range of 0.5-9 mM and 0.1-1.8 mM (R(2) > 0.999), respectively. This work gives new insight into the application of carbon nanodots and provides a general approach for rapid analysis of low-molecular-weight compounds.
authors.affiliation: Beijing National Laboratory for Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China.
authors.forename: Suming
authors.initials: S
authors.lastname: Chen
authors.forename: Huzhi
authors.initials: H
authors.lastname: Zheng
authors.forename: Jianing
authors.initials: J
authors.lastname: Wang
authors.forename: Jian
authors.initials: J
authors.lastname: Hou
authors.forename: Qing
authors.initials: Q
authors.lastname: He
authors.forename: Huihui
authors.initials: H
authors.lastname: Liu
authors.forename: Caiqiao
authors.initials: C
authors.lastname: Xiong
authors.forename: Xianglei
authors.initials: X
authors.lastname: Kong
authors.forename: Zongxiu
authors.initials: Z
authors.lastname: Nie
mesh_terms: D002244:Carbon / Q000737:chemistry*; D005947:Glucose / Q000032:analysis*; D006801:Humans; D053758:Nanoparticles / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D014527:Uric Acid / Q000032:analysis* / Q000652:urine
chemical_list: D014527:Uric Acid; D002244:Carbon; D005947:Glucose