title: Real-time observation of multiple-protein complex formation with single-molecule FRET.
abstract: Current single-molecule techniques do not permit the real-time observation of multiple proteins interacting closely with each other. We here report an approach enabling us to determine the single-molecule fluorescence resonance energy transfer (FRET) kinetics of multiple protein-protein interactions occurring far below the diffraction limit. We observe a strongly cooperative formation of multimeric soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) complexes, which suggests that formation of the first SNARE complex triggers a cascade of SNARE complex formation.
authors.forename: Wooli
authors.initials: W
authors.lastname: Bae
authors.affiliation: National Creative Research Initiative Center for Single-Molecule Systems Biology and Department of Physics, KAIST, Daejeon 305-701, South Korea.
authors.forename: Woori
authors.initials: W
authors.lastname: Bae
authors.forename: Mal-Gi
authors.initials: MG
authors.lastname: Choi
authors.forename: Changbong
authors.initials: C
authors.lastname: Hyeon
authors.forename: Yeon-Kyun
authors.initials: YK
authors.lastname: Shin
authors.forename: Tae-Young
authors.initials: TY
authors.lastname: Yoon
mesh_terms: D031541:Fluorescence Resonance Energy Transfer; D007700:Kinetics; D008958:Models, Molecular; D011485:Protein Binding; D011506:Proteins / Q000737:chemistry*; D013997:Time Factors
chemical_list: D011506:Proteins