title: Effect of chitosan-gluconic acid conjugate/poly(vinyl alcohol) cryogels as wound dressing on partial-thickness wounds in diabetic rats.
abstract: We previously developed chitosan cryogels from chitosan-gluconic acid conjugate without using toxic additives for wound care. In this study, we improved physiological characteristics of the previous cryogels by incorporating poly(vinyl alcohol) that also form cryogels. Mechanical strength of the cryogels was more than two times higher than that of the previous cryogels. Furthermore, the incorporation of poly(vinyl alcohol) enhanced water retention and resistance to degradation of the gels by lysozyme. The cryogels retained the favorable biological properties of the previous cryogels that they accelerate infiltration of inflammatory cells into wound sites. Time period for repairing 50 % of initial area of partial-thickness skin wound treated with the cryogels (4.0 ± 1.1 days) was shorter than those with gauze (6.5 ± 0.3 days) or a commercial hydrogel dressing (5.7 ± 0.3 days). Finally, we confirmed that incorporation of basic fibroblast growth factor into the cryogels was effective to further accelerate wound healing (2.7 ± 1.0 days). These results demonstrate that the cryogels in this study are promising for wound care.
authors.affiliation: Department of Chemical Engineering, Graduate School of Science and Engineering, Kagoshima University, 1-21-40 Korimoto, Kagoshima, 890-0065, Japan, takei@cen.kagoshima-u.ac.jp.
authors.forename: Takayuki
authors.initials: T
authors.lastname: Takei
authors.forename: Hideki
authors.initials: H
authors.lastname: Nakahara
authors.forename: Sadao
authors.initials: S
authors.lastname: Tanaka
authors.forename: Hiroto
authors.initials: H
authors.lastname: Nishimata
authors.forename: Masahiro
authors.initials: M
authors.lastname: Yoshida
authors.forename: Koei
authors.initials: K
authors.lastname: Kawakami
mesh_terms: D000818:Animals; D047070:Bandages, Hydrocolloid; D048271:Chitosan / Q000737:chemistry*; D059985:Cryogels / Q000737:chemistry; D003921:Diabetes Mellitus, Experimental / Q000628:therapy*; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism; D005942:Gluconates / Q000737:chemistry*; D008297:Male; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D009113:Muramidase / Q000737:chemistry; D011142:Polyvinyl Alcohol / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar; D013314:Stress, Mechanical; D014867:Water / Q000737:chemistry; D014945:Wound Healing / Q000187:drug effects
chemical_list: D059985:Cryogels; D005942:Gluconates; D014867:Water; D016222:Fibroblast Growth Factor 2; D011142:Polyvinyl Alcohol; D048271:Chitosan; D009113:Muramidase; C030691:gluconic acid