title: [Effect of strain-producer and cultivation medium on cross antigenic activity of Streptococcus pneumoniae water soluble antigens].
abstract: OBJECTIVE
  Production of water soluble protein-containing antigens from various strains of S. pneumoniae during cultivation in complete and semi-synthetic culture media as well as selection of strains with cross antigenic activity.
  
  
  METHODS
  S. pneumoniae 3, 6A, 6B, 14, 10A, 18A, 19A, 19F, 23F serotype strains were cultivated in brain-heart broth and semi-synthetic medium with addition of aminopeptide for 24 hours at 37 degrees C for the production of water soluble antigens. The antigens were obtained by a method of triple water extraction from acetone dried microbial cells. Chemical composition of preparations, electrophoresis mobility of protein-containing components of preparations and cross antigenic activity in gel immune diffusion reaction by using rabbit hyperimmune sera were studied.
  
  
  RESULTS
  In studies of 10 pneumococcus strains from various serotypes a method of microbial cell inactivation by acetone was selected that allows to produce preparations with high protein content (25.5 - 53.1%). Electrophoretic separation of the preparations revealed difference in the preparations obtained from various pneumococcus strains in the layout of major protein lines in the 8 - 95 kDa range. The most virulent and immunogenic S. pneumoniae strain that during cultivation in semi-synthetic medium was characterized by intraspecies cross antigenic activity and in gel immune diffusion reacted with all the studied sera against 3, 14, 18C, 23F serotype strains was selected.
  
  
  CONCLUSIONS
  The study resulted in the selection of a technologically simple method of production of pneumococcus antigens with high protein content and showed that only 1 of the studied preparations produced from a virulent strain with poorly expressed S. pneumoniae capsule during cultivation in semi-synthetic medium has the highest cross antigenic activity.
authors.forename: E A
authors.initials: EA
authors.lastname: Kurbatova
authors.forename: D S
authors.initials: DS
authors.lastname: Vorob'ev
authors.forename: N B
authors.initials: NB
authors.lastname: Egorova
authors.forename: S I
authors.initials: SI
authors.lastname: Elkina
authors.forename: N G
authors.initials: NG
authors.lastname: Kalina
authors.forename: M M
authors.initials: MM
authors.lastname: Tokarskaia
authors.forename: A P
authors.initials: AP
authors.lastname: Baturo
authors.forename: É E
authors.initials: ÉE
authors.lastname: Romanenko
authors.forename: M E
authors.initials: ME
authors.lastname: Markova
authors.forename: N V
authors.initials: NV
authors.lastname: Grishchenko
authors.forename: N N
authors.initials: NN
authors.lastname: Ovechko
authors.forename: Iu V
authors.initials: IuV
authors.lastname: Volokh
authors.forename: S A
authors.initials: SA
authors.lastname: Zlygostev
authors.forename: N A
authors.initials: NA
authors.lastname: Mikhaĭlova
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification; D001426:Bacterial Proteins / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification; D003470:Culture Media / Q000737:chemistry; D011817:Rabbits; D012995:Solubility; D013296:Streptococcus pneumoniae / Q000737:chemistry / Q000254:growth & development / Q000276:immunology; D014867:Water / Q000737:chemistry
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D003470:Culture Media; D014867:Water