title: Astragalus polysaccharide improves muscle atrophy from dexamethasone- and peroxide-induced injury in vitro.
abstract: Astragalus polysaccharide (APS) is an important bioactive component of Astragalus membranaceus Bunge (Leguminosae) that has been used in traditional Chinese medicine for treating muscle wasting, a serious complication with complex mechanism manifested as myofibers atrophy and satellite cells apoptosis. In this study, the anti-atrophy and anti-apoptotic activity of Astragalus polysaccharide (APS) was characterized in C2C12 skeletal muscle myotubes and myoblasts. APS inhibited dexamethasone-induced atrophy by restoring phosphorylation of Akt, m-TOR, P70s6k, rpS6 and FoxO3A/FoxO1. The targets that protected C2C12 myoblasts from damage by H2O2 were promoting cells proliferation and inhibiting cells apoptosis. The protective mechanisms involved mitochondrial pathway and death receptor pathway. Moreover, Antioxidant effect of APS was also detected in this work. Our findings suggested that APS could be explored as a protective and perhaps as a therapeutic agent in the management of muscle wasting.
authors.affiliation: Department of Traditional Chinese Medicine, ZhuJiang Hospital, Southern Medical University, Guangzhou 510280, China; School of Traditional Chinese Medicine, Southern Medical University, Guangzhou 510515, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Lu
authors.forename: Dong-Tao
authors.initials: DT
authors.lastname: Wang
authors.forename: Ying
authors.initials: Y
authors.lastname: Shi
authors.forename: Yi
authors.initials: Y
authors.lastname: Yin
authors.forename: Lian-Bo
authors.initials: LB
authors.lastname: Wei
authors.forename: Yu-Cong
authors.initials: YC
authors.lastname: Zou
authors.forename: Bo
authors.initials: B
authors.lastname: Huang
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhao
authors.forename: Ming
authors.initials: M
authors.lastname: Wang
authors.forename: Heng
authors.initials: H
authors.lastname: Wan
authors.forename: Cheng-Jie
authors.initials: CJ
authors.lastname: Li
authors.forename: Jian-Xin
authors.initials: JX
authors.lastname: Diao
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D051017:Apoptosis Regulatory Proteins / Q000378:metabolism; D027883:Astragalus Plant / Q000737:chemistry*; D016923:Cell Death / Q000187:drug effects; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D003907:Dexamethasone / Q000009:adverse effects; D004789:Enzyme Activation / Q000187:drug effects; D006861:Hydrogen Peroxide / Q000009:adverse effects; D051379:Mice; D018485:Muscle Fibers, Skeletal / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D009133:Muscular Atrophy / Q000139:chemically induced / Q000378:metabolism*; D032448:Myoblasts, Skeletal / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D011134:Polysaccharides / Q000494:pharmacology*; D054562:Protein Tyrosine Phosphatase, Non-Receptor Type 1 / Q000378:metabolism; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D058570:TOR Serine-Threonine Kinases / Q000378:metabolism
chemical_list: D051017:Apoptosis Regulatory Proteins; D011134:Polysaccharides; D017382:Reactive Oxygen Species; D003907:Dexamethasone; D006861:Hydrogen Peroxide; D051057:Proto-Oncogene Proteins c-akt; D058570:TOR Serine-Threonine Kinases; D054562:Protein Tyrosine Phosphatase, Non-Receptor Type 1
keywords: Apoptosis; Astragalus polysaccharide; Atrophy; Myoblast; Myotubes