title: Biological evaluation of polyvinyl alcohol hydrogel crosslinked by polyurethane chain for cartilage tissue engineering in rabbit model.
abstract: Polyvinyl alcohol (PVA) hydrogel chains were crosslinked by urethane pre-polymer (PPU) in order to fabricate a new substitute for cartilage lesions. The microscopy images showed that the cultured chondrocytes had spherical morphology on PVA-PPU sample after 4 weeks of isolation in vitro. The alcian blue and safranin O staining proved the presence of proteoglycan on the surface of PVA-PPU sample secreted by cultured chondrocytes. This was confirmed by the detection of sulfate ions in the wavelength dispersive X-ray (WDX) analysis. In addition, the expression of collagen type II and aggrecan were observed in chondrocytes cultured on PVA-PPU by RT-PCR. Moreover, the implantation of the PVA-PPU sample with autologous cultured chondrocytes revealed the formation of neocartilage tissue in a rabbit model during 12 weeks follow up. In conclusion, the results verified that isolated chondrocytes cultured on PVA-PPU retain their original phenotype and this composition can be considered as promising substrate for cartilage tissue engineering.
authors.affiliation: National Cell Bank of Iran, Pasteur Institute of Iran, 13164, Tehran, Iran, mashokrgozar@yahoo.com.
authors.forename: Mohammad Ali
authors.initials: MA
authors.lastname: Shokrgozar
authors.forename: Shahin
authors.initials: S
authors.lastname: Bonakdar
authors.forename: Mohammad Mehdi
authors.initials: MM
authors.lastname: Dehghan
authors.forename: Shahriar Hojjati
authors.initials: SH
authors.lastname: Emami
authors.forename: Leila
authors.initials: L
authors.lastname: Montazeri
authors.forename: Shahram
authors.initials: S
authors.lastname: Azari
authors.forename: Mohsen
authors.initials: M
authors.lastname: Rabbani
mesh_terms: D000327:Adsorption; D053674:Aggrecans / Q000737:chemistry*; D000423:Alcian Blue / Q000737:chemistry; D000818:Animals; D002356:Cartilage / Q000378:metabolism*; D002358:Cartilage, Articular / Q000166:cytology; D002454:Cell Differentiation; D019902:Chondrocytes / Q000166:cytology; D003094:Collagen / Q000737:chemistry; D003432:Cross-Linking Reagents / Q000737:chemistry; D018625:Microscopy, Atomic Force; D010619:Phenazines / Q000737:chemistry; D011137:Polystyrenes / Q000737:chemistry; D011140:Polyurethanes / Q000737:chemistry*; D011142:Polyvinyl Alcohol / Q000737:chemistry*; D011312:Pressure; D011509:Proteoglycans / Q000737:chemistry; D011817:Rabbits; D013431:Sulfates / Q000737:chemistry; D013718:Tensile Strength; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D053674:Aggrecans; D003432:Cross-Linking Reagents; D010619:Phenazines; D011137:Polystyrenes; D011140:Polyurethanes; D011509:Proteoglycans; D013431:Sulfates; C062364:polyvinyl alcohol hydrogel; D011142:Polyvinyl Alcohol; D003094:Collagen; D000423:Alcian Blue; C009195:safranine T