title: DNA interaction of CuII, NiII and ZnII functionalized salphen complexes: studies by linear dichroism, gel electrophoresis and PCR.
abstract: The interaction of salphen-type NiII, CuII and ZnII complexes with native DNA was investigated by exploiting linear dichroism experiments. The NiII complex behaves as a typical intercalator, binding strongly and stiffening and unwinding the DNA. The strength of the DNA interaction is slightly weaker for the copper complex and much weaker for the zinc complex. Plasmid-DNA gel electrophoresis experiments indicated that while CuII and ZnII complexes do not induce the unwinding of supercoiled DNA, the NiII complex has a nuclease activity without the addition of external agents. On the other hand, as shown in the PCR assays, we demonstrate that, at the used concentrations, only the CuII complex is able to inhibit the DNA amplification mediated by Taq DNA polymerase. In this paper we have also reported a detailed characterization of the three compounds including 2D-NMR and ESI-mass experiments and X-ray single crystal structure of the copper and nickel compounds.
authors.affiliation: School of Chemistry, University of Birmingham Edgbaston, Birmingham B152TT, UK.
authors.forename: Alessio
authors.initials: A
authors.lastname: Terenzi
authors.forename: Cosimo
authors.initials: C
authors.lastname: Ducani
authors.forename: Louise
authors.initials: L
authors.lastname: Male
authors.forename: Giampaolo
authors.initials: G
authors.lastname: Barone
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Hannon
mesh_terms: D040761:Biomimetic Materials / Q000737:chemistry / Q000378:metabolism; D002942:Circular Dichroism; D056831:Coordination Complexes / Q000737:chemistry* / Q000378:metabolism; D003300:Copper / Q000737:chemistry*; D018360:Crystallography, X-Ray; D004247:DNA / Q000737:chemistry*; D003851:Deoxyribonucleases / Q000378:metabolism; D004587:Electrophoresis, Agar Gel; D008968:Molecular Conformation; D009532:Nickel / Q000737:chemistry*; D010655:Phenylenediamines / Q000737:chemistry*; D016133:Polymerase Chain Reaction; D015032:Zinc / Q000737:chemistry*
chemical_list: D056831:Coordination Complexes; D010655:Phenylenediamines; C545695:salphen; D003300:Copper; D009532:Nickel; D004247:DNA; D003851:Deoxyribonucleases; D015032:Zinc