title: Low brain DHA content worsens sensorimotor outcomes after TBI and decreases TBI-induced Timp1 expression in juvenile rats.
abstract: The effects of dietary modulation of brain DHA content on outcomes after TBI were examined in a juvenile rat model. Long-Evans rats with normal or diet-induced decreases in brain DHA were subjected to a controlled cortical impact or sham surgery on postnatal day 17. Rats with the greatest decreases in brain DHA had the poorest sensorimotor outcomes after TBI. Ccl2, Gfap, and Mmp 9 mRNA levels, and MMP-2 and -9 enzymatic activities were increased after TBI regardless of brain DHA level. Lesion volume was not affected by brain DHA level. In contrast, TBI-induced Timp1 expression was lower in rats on the Deficient diet and correlated with brain DHA level. These data suggest that decreased brain DHA content contributes to poorer sensorimotor outcomes after TBI through a mechanism involving modulation of Timp1 expression.
authors.affiliation: Department of Pharmacology, Toxicology, and Therapeutics, University of Kansas Medical Center, 3901 Rainbow Blvd., Kansas City, KS 66160, USA. krussell@kumc.edu
authors.forename: Kristin L
authors.initials: KL
authors.lastname: Russell
authors.forename: Nancy E J
authors.initials: NE
authors.lastname: Berman
authors.forename: Beth
authors.initials: B
authors.lastname: Levant
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism*; D001930:Brain Injuries / Q000378:metabolism* / Q000503:physiopathology; D004281:Docosahexaenoic Acids / Q000378:metabolism*; D056228:Feedback, Sensory / Q000502:physiology; D020782:Matrix Metalloproteinases; D051381:Rats; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000378:metabolism*
chemical_list: D019715:Tissue Inhibitor of Metalloproteinase-1; D004281:Docosahexaenoic Acids; D020782:Matrix Metalloproteinases
keywords: Docosahexaenoic acid; Juvenile; Matrix metalloproteinase; Sensorimotor; TIMP-1; Traumatic brain injury