title: An amperometric biosensor for glucose detection from glucose oxidase immobilized in polyaniline-polyvinylsulfonate-potassium ferricyanide film.
abstract: In this study, a novel amperometric glucose biosensor with immobilization of glucose oxidase on electrochemically polymerized polyaniline-polyvinylsulphonate-potassium ferricyanide (Pani-Pvs-Fc) films has been accomplished via the entrapment technique. Potassium ferricyanide was used as the mediator. Determination of glucose was carried out by the oxidation of potassium ferrocyanide at 0.3 V vs. Ag/AgCl. The effects of pH and temperature were investigated, and the optimum pH value was found to be 7.5. The storage stability and the operational stability of the enzyme electrode were also studied.
authors.affiliation: Department of Chemistry, Faculty of Sciences, Gazi University , Ankara , Türkiye.
authors.forename: Fatma
authors.initials: F
authors.lastname: Arslan
authors.forename: Umut
authors.initials: U
authors.lastname: Beskan
mesh_terms: D000814:Aniline Compounds / Q000737:chemistry*; D001234:Aspergillus niger / Q000201:enzymology; D015374:Biosensing Techniques / Q000379:methods*; D002138:Calibration; D004563:Electrochemistry; D004800:Enzymes, Immobilized / Q000737:chemistry* / Q000378:metabolism; D005292:Ferricyanides / Q000737:chemistry*; D005947:Glucose / Q000032:analysis* / Q000737:chemistry; D005949:Glucose Oxidase / Q000737:chemistry* / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D011145:Polyvinyls / Q000737:chemistry*; D013451:Sulfonic Acids / Q000737:chemistry*; D013696:Temperature
chemical_list: D000814:Aniline Compounds; D004800:Enzymes, Immobilized; D005292:Ferricyanides; D011145:Polyvinyls; D013451:Sulfonic Acids; C416807:polyaniline; C008959:lyapolate; D005949:Glucose Oxidase; D005947:Glucose; C028033:potassium ferricyanide
keywords: amperometric biosensor; ferricyanide; glucose; polyaniline; polyvinylsulphonate