title: Lentiviral transduction of cultured microglia.
abstract: Microglial cells are the resident immune-related glial cells of the central nervous system (CNS) that are crucial for maintaining homeostasis and sensing pathological alterations in the nervous system. To improve our understanding of the biological function of microglia, gene-transfer techniques have been improved and become widely used over the past several years. Here, we describe lentiviral-mediated transduction as a valuable tool for transduction of cultured microglial cells.
authors.affiliation: Kyushu University, Fukuoda, Japan.
authors.forename: Takahiro
authors.initials: T
authors.lastname: Masuda
authors.forename: Makoto
authors.initials: M
authors.lastname: Tsuda
authors.forename: Hidetoshi
authors.initials: H
authors.lastname: Tozaki-Saitoh
authors.forename: Kazuhide
authors.initials: K
authors.lastname: Inoue
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D006801:Humans; D016086:Lentivirus / Q000235:genetics*; D017628:Microglia / Q000166:cytology* / Q000378:metabolism*; D014161:Transduction, Genetic / Q000379:methods*