title: [Expression and significance of vitamin D and its receptor mRNA in the peripheral blood of initial immune thrombocytopenic patients].
abstract: To investigate the effect of vitamin D in pathogenesis and clinical treatment of patients with immune thrombocytopenic (ITP), ELISA was used to detect the level of 25-hydroxylate vitamin D3[25(OH)D3] and 1,25-dihydroxyvitamin D3[1,25(OH)2D3] in peripheral blood from 45 ITP patients and 30 normal controls. Vitamin D receptor (VDR) mRNA expression was detected by RT-PCR. The results showed that the levels of 25(OH)D3 (10.6 ± 7.7 ng/ml) and 1,25(OH)2D3 (69.9 ± 29.0 pg/ml) in peripheral blood of the initial ITP patients were lower than those in peripheral blood of normal controls (13.7 ± 9.1 ng/ml, 87.3 ± 19.9 pg/ml) (P < 0.05). The expression of VDR gene obviously increased in peripheral blood of initial ITP patients (1.588 ± 0.127), as compared with that in peripheral blood of normal controls (1.055 ± 0.734) (P < 0.05). It is concluded that vitamin D level and its receptor expression may play an important role in ITP, and vitamin D and its similarities may be a new agent to treat patients with ITP.
authors.affiliation: Department of Hematology, The Affiliated Hospital of Qingdao University Medical College, Qingdao, Shandong Province, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Mu
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Zhong-Guang
authors.initials: ZG
authors.lastname: Cui
authors.forename: Ai-Hua
authors.initials: AH
authors.lastname: Sui
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D016022:Case-Control Studies; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D012333:RNA, Messenger / Q000235:genetics; D018167:Receptors, Calcitriol / Q000097:blood*; D013921:Thrombocytopenia / Q000097:blood*; D014807:Vitamin D / Q000097:blood*; D055815:Young Adult
chemical_list: D012333:RNA, Messenger; D018167:Receptors, Calcitriol; D014807:Vitamin D