title: [Effects of geniposide on SNP-induced apoptosis of chondrocyte and cell cycle].
abstract: OBJECTIVE
  To study the effects of Geniposide on SNP(sodium nitroprusside)-induced apoptosis of chondrocyte in vitro and cell cycle.
  
  
  METHODS
  The chondrocyte of three-week-old SD rats were separated and cultivated. The second generation of chondrocyte cells were involved in experiment. Chondrocyte proliferation was measured by assay; flow cytometer were adopted to observe cell cycle and apoptosis rate; NO examination adopted nitrate reductase method.
  
  
  RESULTS
  Geniposide could significantly decrease the percentage of SNP-induced chondrocytes in G0/G1 phase and increased percentage in S phase and G2/M phase. The apoptosis of chondrocyte and the concentration of NO in the culture supernatants was reduced significantly (r=0.917, P<0.01).
  
  
  CONCLUSIONS
  Geniposide could impact SNP-induced apoptosis of chondrocyte by reducing the concentration of NO in the culture supernatants, promoting proliferation of chondrocytes, which is a probable and important mechanism of Geniposide preventing osteoarthritis.
authors.affiliation: Department of Orthopaedics, China Three Gorges University, Yichang Central People's Hospital, yichang, Hubei, China.
authors.forename: Wan-Jun
authors.initials: WJ
authors.lastname: Chen
authors.forename: Tong-Zhu
authors.initials: TZ
authors.lastname: Bao
authors.forename: Ken
authors.initials: K
authors.lastname: Chen
authors.forename: Chang-Mou
authors.initials: CM
authors.lastname: Zhu
authors.forename: Feng
authors.initials: F
authors.lastname: Wan
authors.forename: Yu-Lin
authors.initials: YL
authors.lastname: Tan
authors.forename: Fei
authors.initials: F
authors.lastname: Yan
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002453:Cell Cycle / Q000187:drug effects; D019902:Chondrocytes / Q000187:drug effects* / Q000502:physiology; D005260:Female; D039823:Iridoids / Q000494:pharmacology* / Q000627:therapeutic use; D008297:Male; D009599:Nitroprusside / Q000494:pharmacology*; D010003:Osteoarthritis / Q000188:drug therapy; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D039823:Iridoids; C007835:geniposide; D009599:Nitroprusside