title: Heritable genome editing in C. elegans via a CRISPR-Cas9 system.
abstract: We report the use of clustered, regularly interspaced, short palindromic repeats (CRISPR)-associated endonuclease Cas9 to target genomic sequences in the Caenorhabditis elegans germ line using single-guide RNAs that are expressed from a U6 small nuclear RNA promoter. Our results demonstrate that targeted, heritable genetic alterations can be achieved in C. elegans, providing a convenient and effective approach for generating loss-of-function mutants.
authors.affiliation: Department of Genetics, Harvard Medical School, Boston, Massachusetts, USA.
authors.forename: Ari E
authors.initials: AE
authors.lastname: Friedland
authors.forename: Yonatan B
authors.initials: YB
authors.lastname: Tzur
authors.forename: Kevin M
authors.initials: KM
authors.lastname: Esvelt
authors.forename: Monica P
authors.initials: MP
authors.lastname: Colaiácovo
authors.forename: George M
authors.initials: GM
authors.lastname: Church
authors.forename: John A
authors.initials: JA
authors.lastname: Calarco
mesh_terms: D000818:Animals; D017173:Caenorhabditis elegans / Q000235:genetics*; D005818:Genetic Engineering / Q000379:methods; D016678:Genome; D055029:Inverted Repeat Sequences; D011401:Promoter Regions, Genetic; D012342:RNA, Small Nuclear / Q000235:genetics*; D029961:Zebrafish Proteins / Q000235:genetics; D058727:RNA, Small Untranslated
chemical_list: D012342:RNA, Small Nuclear; D029961:Zebrafish Proteins; C503768:unc119a protein, zebrafish; D058727:RNA, Small Untranslated