title: Ratiometric fluorescent probes for detection of intracellular singlet oxygen.
abstract: We have developed a series of molecular probes for the fluorescent detection of singlet dioxygen ((1)O2). The probes, based on asymmetrically substituted 1,3-diarylisobenzofurans, undergo the [2 + 4] cycloaddition reaction with (1)O2, producing ratiometric fluorescent responses. Two-photon fluorescence microscope experiments demonstrated the biological utility of the probes for the visualization of endogenous (1)O2 in macrophage cells.
authors.affiliation: Department of Bioinspired Science, Ewha Womans University, Seoul 120-750, Korea.
authors.forename: Dayoung
authors.initials: D
authors.lastname: Song
authors.forename: Somin
authors.initials: S
authors.lastname: Cho
authors.forename: Yejee
authors.initials: Y
authors.lastname: Han
authors.forename: Youngmin
authors.initials: Y
authors.lastname: You
authors.forename: Wonwoo
authors.initials: W
authors.lastname: Nam
mesh_terms: D061565:Cycloaddition Reaction; D005456:Fluorescent Dyes / Q000737:chemistry*; D008264:Macrophages / Q000737:chemistry*; D008856:Microscopy, Fluorescence / Q000379:methods*; D017785:Photons; D026082:Singlet Oxygen / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
chemical_list: D005456:Fluorescent Dyes; D026082:Singlet Oxygen