title: Enhanced xylose fermentation capacity related to an altered glucose sensing and repression network in a recombinant Saccharomyces cerevisiae.
abstract: The co-fermentation of glucose and xylose is one of the issues in decreasing the price of biofuel or chemicals produced from lignocellulosic materials. A glucose and xylose co-utilizing Saccharomyces cerevisiae was obtained through rational genetic manipulation. Non-rational evolution in xylose was performed, and the xylose utilization efficiency of the engineered strain was significantly enhanced. The results of transcriptome study suggested that Snf1/Mig1-mediated regulation, a part of glucose sensing and repression network, was altered in the evolved strain and might be related to the enhancement of xylose utilization.
authors.affiliation: The State Key Laboratory of Microbial Technology; Shandong University; Jinan, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Shen
authors.affiliation: The State Key Laboratory of Microbial Technology; Shandong University; Jinan, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Hou
authors.affiliation: The State Key Laboratory of Microbial Technology; Shandong University; Jinan, China.
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Bao
mesh_terms: D005285:Fermentation; D020869:Gene Expression Profiling; D005818:Genetic Engineering; D005947:Glucose / Q000378:metabolism; D010766:Phosphorylation; D011994:Recombinant Proteins / Q000235:genetics* / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics* / Q000378:metabolism; D014994:Xylose / Q000378:metabolism*
chemical_list: D011994:Recombinant Proteins; D029701:Saccharomyces cerevisiae Proteins; D014994:Xylose; D005947:Glucose
keywords: Mig1; Saccharomyces cerevisiae; Snf1; adaptive evolution; ethanol; glucose repression; xylose fermentation