title: Hypothermia inhibits expression of CD11b (MAC-1) and CD162 (PSGL-1) on monocytes during extracorporeal circulation.
abstract: OBJECTIVE
  The aim of the present study was to investigate the effect of different hypothermic temperatures on the expression of cellular adhesion molecules on leukocytes.
  
  
  METHODS
  Circulation of blood from six volunteers was performed in an extracorporeal circulation model at 36°C, 28°C and 18°C for 30 minutes. Expression of CD11b, CD54 and CD162 on monocytes was measured using flow cytometry.
  
  
  RESULTS
  Expression of CD11b significantly decreased at 18°C and at 28°C compared to 36°C. A significant reduction of CD162 expression was found at 18°C compared to 28°C and 36°C and at 28°C compared to 36°C. No association was found between temperature and expression of CD54.
  
  
  CONCLUSIONS
  Expression of CD11b and CD162 on monocytes has a temperature-dependent regulation, with decreased expression during hypothermia, which may result in an inhibition of leukocyte-endothelial and leukocyte-platelet interaction. This beneficial effect may influence the extracorporeal circulation-related inflammatory response and tissue damage.
authors.affiliation: Pharmacy Department, University Hospital of Heidelberg, Heidelberg, Germany.
authors.forename: Stefanie
authors.initials: S
authors.lastname: Swoboda
authors.forename: Joachim
authors.initials: J
authors.lastname: Gruettner
authors.forename: Siegfried
authors.initials: S
authors.lastname: Lang
authors.forename: Hans-Peter
authors.initials: HP
authors.lastname: Wendel
authors.forename: Martin E
authors.initials: ME
authors.lastname: Beyer
authors.forename: Eva
authors.initials: E
authors.lastname: Griesel
authors.forename: Hans-Martin
authors.initials: HM
authors.lastname: Hoffmeister
authors.forename: Thomas
authors.initials: T
authors.lastname: Walter
mesh_terms: D039481:CD11b Antigen / Q000378:metabolism*; D005112:Extracorporeal Circulation; D005786:Gene Expression Regulation; D006801:Humans; D007035:Hypothermia / Q000235:genetics / Q000378:metabolism*; D008297:Male; D008562:Membrane Glycoproteins / Q000378:metabolism*; D009000:Monocytes / Q000378:metabolism*; D013696:Temperature
chemical_list: D039481:CD11b Antigen; D008562:Membrane Glycoproteins; C084882:P-selectin ligand protein
keywords: CD11b; CD162; CD54; PSGL-1; cardiopulmonary bypass; extracorporeal circulation; hypothermia