title: Improved in vitro and in vivo cutaneous delivery of protoporphyrin IX from PLGA-based nanoparticles.
abstract: We report the development of D, L lactic co-glycolic acid) (PLGA)-based nanoparticles (NPs) for topical delivery of protoporphyrin IX (PpIX), a photosensitizer (PS), in treatments like photodynamic therapy (PDT) of skin cancers. PpIX-NPs were obtained in ~75.0% yield, encapsulation efficiency of 67.7%, drug content of 50.3 μg mg(-1), average diameter of 290 nm maintained up to 30 days and a zeta potential of 32.3 mV. Sustained in vitro release of PpIX through artificial membranes following Higuchi kinetics was kept up to 10 days. In vitro retentions of PpIX both in stratum corneum (SC) and epidermis + dermis ([EP + D]) were higher from NPs (23.0 and 10.0 times, respectively) compared to control solutions at all times. Quantification of PpIX by extraction, after in vivo skin application of NPs-PpIX on hairless mice, showed higher retention of the PS both in SC and in [EP + D] (3.0 and 2.0 times, respectively) compared to control solutions. Taken together, the results indicate that NPs are suitable for PpIX encapsulation showing minimal permeation through the skin and a localized effect, characteristics of a potential and promising delivery system for PDT-associated treatments of skin cancers, photodiagnosis and their off-label uses.
authors.affiliation: School of Pharmacy, Federal University of Rio de Janeiro, Rio de Janeiro, SP, Brazil.
authors.forename: Carolina L
authors.initials: CL
authors.lastname: da Silva
authors.forename: José O
authors.initials: JO
authors.lastname: Del Ciampo
authors.forename: Fábia C
authors.initials: FC
authors.lastname: Rossetti
authors.forename: Maria V L B
authors.initials: MV
authors.lastname: Bentley
authors.forename: Maria B R
authors.initials: MB
authors.lastname: Pierre
mesh_terms: D000818:Animals; D004337:Drug Carriers; D066298:In Vitro Techniques; D019344:Lactic Acid / Q000737:chemistry*; D051379:Mice; D008812:Mice, Hairless; D053758:Nanoparticles; D010539:Permeability; D017319:Photosensitizing Agents / Q000008:administration & dosage* / Q000493:pharmacokinetics; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011524:Protoporphyrins / Q000008:administration & dosage* / Q000493:pharmacokinetics; D012867:Skin / Q000378:metabolism*
chemical_list: D004337:Drug Carriers; D017319:Photosensitizing Agents; D011524:Protoporphyrins; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; C028025:protoporphyrin IX