title: Cancer-related genes transcriptionally induced by the fungicide penconazole.
abstract: Penconazole is a systemic triazole fungicide mainly used on grapes. The UE Maximum Residue Level (MRL) for penconazole is set at 0.2ppm in wine and grapes. In the aim of identifying potential biomarkers of exposure to penconazole and possibly highlighting its endocrine disrupting mode of action, we used a transcriptomics-based approach to detect genes, that are transcriptionally modulated by penconazole, by using an appropriate in vitro model. T-47D cells were treated with commercial penconazole or penconazole contaminated grape extracts for 4h at doses close to the MRL. The whole-genome transcriptomic profile was assessed by using genome 44K oligo-microarray slides. The list of common genes generated by the two treatments could be representative of potential markers of exposure. In order to understand the role of these genes in key events related to adversity, a pathway analysis was performed on a list of genes with the same modulation trend (up or down). The analysis returned a set of genes involved in Thyroid Cancer Pathway, thus confirming a role of penconazole in endocrine disrupting mediated effects and strongly suggesting a possible mode of action in thyroid carcinogenesis.
authors.affiliation: Center for Environmental Carcinogenesis and Risk Assessment, Environmental Protection and Health Prevention Agency - Emilia-Romagna Region (ER-EPA), Viale Filopanti 22, 40126 Bologna, Italy.
authors.forename: Stefania
authors.initials: S
authors.lastname: Perdichizzi
authors.forename: Maria Grazia
authors.initials: MG
authors.lastname: Mascolo
authors.forename: Paola
authors.initials: P
authors.lastname: Silingardi
authors.forename: Elena
authors.initials: E
authors.lastname: Morandi
authors.forename: Francesca
authors.initials: F
authors.lastname: Rotondo
authors.forename: Angela
authors.initials: A
authors.lastname: Guerrini
authors.forename: Luciana
authors.initials: L
authors.lastname: Prete
authors.forename: Monica
authors.initials: M
authors.lastname: Vaccari
authors.forename: Annamaria
authors.initials: A
authors.lastname: Colacci
mesh_terms: D001943:Breast Neoplasms / Q000378:metabolism; D045744:Cell Line, Tumor; D052244:Endocrine Disruptors / Q000737:chemistry / Q000633:toxicity*; D005260:Female; D005659:Fungicides, Industrial / Q000737:chemistry / Q000633:toxicity*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000187:drug effects*; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis; D010573:Pesticide Residues / Q000737:chemistry / Q000633:toxicity; D013964:Thyroid Neoplasms / Q000235:genetics; D014230:Triazoles / Q000737:chemistry / Q000633:toxicity*; D027843:Vitis / Q000737:chemistry; D014920:Wine / Q000032:analysis
chemical_list: D052244:Endocrine Disruptors; D005659:Fungicides, Industrial; D010573:Pesticide Residues; D014230:Triazoles; C087058:penconazole
keywords: Contaminated food commodities; Endocrine disruptors; MRL; Maximum Residue Level; Molecular markers; Penconazole; UC; untreated cells