title: Dietary α-linolenic acid increases the platelet count in ApoE-/- mice by reducing clearance.
abstract: Previously we reported that dietary intake of alpha-linolenic acid (ALA) reduces atherogenesis and inhibits arterial thrombosis. Here, we analyze the substantial increase in platelet count induced by ALA and the mechanisms of reduced platelet clearance. Eight-week-old male apolipoprotein E knockout (ApoE(-/-)) mice were fed a 0.21g% cholesterol diet complemented by either a high- (7.3g%) or low-ALA (0.03g%) content. Platelet counts doubled after 16 weeks of ALA feeding, whereas the bleeding time remained similar. Plasma glycocalicin and glycocalicin index were reduced, while reticulated platelets, thrombopoietin, and bone marrow megakaryocyte colony-forming units remained unchanged. Platelet contents of liver and spleen were substantially reduced, without affecting macrophage function and number. Glycoprotein Ib (GPIb) shedding, exposure of P-selectin, and activated integrin αIIbβ3 upon activation with thrombin were reduced. Dietary ALA increased the platelet count by reducing platelet clearance in the reticulo-endothelial system. The latter appears to be mediated by reduced cleavage of GPIb by tumor necrosis factor-α-converting enzyme and reduced platelet activation/expression of procoagulant signaling. Ex vivo, there was less adhesion of human platelets to von Willebrand factor under high shear conditions after ALA treatment. Thus, ALA may be a promising tool in transfusion medicine and in high turnover/high activation platelet disorders.
authors.affiliation: Laboratory for Platelet Research, Cardiovascular Research, Institute of Physiology, University of Zurich, Switzerland.
authors.forename: Simona
authors.initials: S
authors.lastname: Stivala
authors.forename: Martin F
authors.initials: MF
authors.lastname: Reiner
authors.forename: Christine
authors.initials: C
authors.lastname: Lohmann
authors.forename: Thomas F
authors.initials: TF
authors.lastname: Lüscher
authors.forename: Christian M
authors.initials: CM
authors.lastname: Matter
authors.forename: Juerg H
authors.initials: JH
authors.lastname: Beer
mesh_terms: D051722:ADAM Proteins / Q000378:metabolism; D000072198:ADAM17 Protein; D000821:Animal Feed; D000818:Animals; D001057:Apolipoproteins E / Q000235:genetics*; D050197:Atherosclerosis / Q000235:genetics* / Q000378:metabolism; D001792:Blood Platelets / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D008099:Liver / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D019007:P-Selectin / Q000378:metabolism; D015539:Platelet Activation; D010973:Platelet Adhesiveness; D010976:Platelet Count; D019039:Platelet Glycoprotein GPIIb-IIIa Complex / Q000378:metabolism; D019038:Platelet Glycoprotein GPIb-IX Complex / Q000378:metabolism / Q000502:physiology; D015398:Signal Transduction; D013154:Spleen / Q000378:metabolism; D017962:alpha-Linolenic Acid / Q000627:therapeutic use*
chemical_list: D001057:Apolipoproteins E; D019007:P-Selectin; D019039:Platelet Glycoprotein GPIIb-IIIa Complex; D019038:Platelet Glycoprotein GPIb-IX Complex; C023592:glycocalicin; D017962:alpha-Linolenic Acid; D051722:ADAM Proteins; D000072198:ADAM17 Protein