title: [Effect of folic acid in preventing aberrant methylation of fetal endometriosis susceptibility gene HOXA10].
abstract: OBJECTIVE
  To detect aberrant methylation in the promoter region of fetal endometriosis susceptibility gene homeobox-10 (HOXA10) in women with and without folic acid supplementation and explore the effect of folic acid in optimizing intrauterine environment.
  
  
  METHODS
  Thirty-six cord blood specimens were collected between January, 2010 and December, 2012 from pregnant women with endometriosis, including 22 with folic acid treatment and 15 without. Methylation-specific polymerase chain reaction (MSP) and bisulfite salt modified sequencing (BSP) were employed to detect aberrant methylation of HOXA10 gene in these specimens.
  
  
  RESULTS
  The methylation rate of HOXA10 gene differed significantly between pregnant women with endometriosis taking folic acid and those who did (P<0.05).
  
  
  CONCLUSIONS
  Folic acid treatment can significantly reduce the methylation rate of fetal endometriosis susceptibility gene HOXA10.
authors.affiliation: Department of Obstetrics and Gynecology, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China. liumb1972@126.com
authors.forename: Mubiao
authors.initials: M
authors.lastname: Liu
authors.forename: Xuemei
authors.initials: X
authors.lastname: Huang
authors.forename: Surong
authors.initials: S
authors.lastname: Xu
authors.forename: Lei
authors.initials: L
authors.lastname: Li
mesh_terms: D019175:DNA Methylation / Q000187:drug effects*; D004715:Endometriosis / Q000235:genetics / Q000378:metabolism*; D005260:Female; D005333:Fetus / Q000378:metabolism; D005492:Folic Acid / Q000494:pharmacology* / Q000627:therapeutic use; D000080905:Homeobox A10 Proteins; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D011247:Pregnancy; D011401:Promoter Regions, Genetic
chemical_list: D000080905:Homeobox A10 Proteins; D018398:Homeodomain Proteins; C069634:HOXA10 protein, human; D005492:Folic Acid