title: A mass-differentiated library strategy for identification of sugar nucleotidyltransferase activities from cell lysates.
abstract: Sugar nucleotidyltransferases, or nucleotide sugar pyrophosphorylases, are ubiquitous enzymes whose activities have been correlated to disease states and pathogen virulence. Here we report a rapid "one-pot" method to identify a range of sugar nucleotidyltransferase activities of purified proteins or in cell lysates using a mass-differentiated carbohydrate library designed for mass spectrometry-based analysis.
authors.affiliation: Department of Chemistry, Iowa State University, Ames, IA 50011, USA.
authors.forename: Kwang-Seuk
authors.initials: KS
authors.lastname: Ko
authors.forename: Rahman M
authors.initials: RM
authors.lastname: Mizanur
authors.forename: Joy M
authors.initials: JM
authors.lastname: Jackson
authors.forename: Lin
authors.initials: L
authors.lastname: Liu
authors.forename: Nicola L B
authors.initials: NL
authors.lastname: Pohl
mesh_terms: D050260:Carbohydrate Metabolism; D004926:Escherichia coli / Q000166:cytology* / Q000201:enzymology*; D015394:Molecular Structure; D009713:Nucleotidyltransferases / Q000737:chemistry / Q000378:metabolism*; D054852:Small Molecule Libraries / Q000737:chemistry / Q000378:metabolism*; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D054852:Small Molecule Libraries; D009713:Nucleotidyltransferases
keywords: Carbohydrate libraries; Enzyme assay; Mass spectrometry; Sugar nucleotidyltransferases