title: Beneficial effects of β-conglycinin on renal function and nephrin expression in early streptozotocin-induced diabetic nephropathy rats.
abstract: The objective of the present study was to investigate the effects of β-conglycinin and soya isoflavones on diabetic nephropathy (DN). DN was induced by an intravenous injection of streptozotocin (25 mg/kg) in spontaneously hypertensive rats. DN rats were divided into a non-diabetic group (C, control group) and three DN groups (D, DN with control diet; B, DN+control diet with one-eighth of casein replaced by β-conglycinin as the protein source; and I, DN+control diet with 0·01 % soya isoflavones). After a 4-week experimental period, we found that fasting blood sugar and plasma and kidney advanced glycation end product levels and 24 h urinary protein excretion of the B group were significantly lower than those of the D group and insulin sensitivity and nephrin expression of the B group were significantly higher than those of the D group. In addition, systolic blood pressure, angiotensin-converting enzyme activity, angiotensin II level and plasma TAG level of the B group were significantly lower than those of the D group, whereas only the levels of plasma TAG and thiobarbituric acid-reactive substances of the I group were lower than those of the D group. In conclusion, β-conglycinin may be beneficial for retarding DN progression and this effect cannot be completely explained by its isoflavone content.
authors.affiliation: Department of Nutrition, I-Shou University, Kaohsiung 824, Taiwan, ROC.
authors.forename: Hsin-Yi
authors.initials: HY
authors.lastname: Yang
authors.affiliation: School of Nutrition and Health Sciences, Taipei Medical University, Taipei 110, Taiwan, ROC.
authors.forename: Lin-Yi
authors.initials: LY
authors.lastname: Wu
authors.affiliation: School of Nutrition and Health Sciences, Taipei Medical University, Taipei 110, Taiwan, ROC.
authors.forename: Wan-Ju
authors.initials: WJ
authors.lastname: Yeh
authors.affiliation: School of Nutrition and Health Sciences, Taipei Medical University, Taipei 110, Taiwan, ROC.
authors.forename: Jiun-Rong
authors.initials: JR
authors.lastname: Chen
mesh_terms: D000804:Angiotensin II / Q000097:blood; D000818:Animals; D052179:Antigens, Plant / Q000494:pharmacology / Q000627:therapeutic use*; D001786:Blood Glucose / Q000378:metabolism; D001794:Blood Pressure / Q000187:drug effects; D003921:Diabetes Mellitus, Experimental / Q000150:complications / Q000178:diet therapy / Q000378:metabolism / Q000503:physiopathology; D003924:Diabetes Mellitus, Type 2 / Q000150:complications / Q000178:diet therapy / Q000378:metabolism / Q000503:physiopathology; D003928:Diabetic Nephropathies / Q000178:diet therapy* / Q000378:metabolism / Q000503:physiopathology; D004044:Dietary Proteins / Q000494:pharmacology / Q000627:therapeutic use*; D005916:Globulins / Q000494:pharmacology / Q000627:therapeutic use*; D017127:Glycation End Products, Advanced / Q000378:metabolism; D007333:Insulin Resistance; D007529:Isoflavones / Q000494:pharmacology*; D007668:Kidney / Q000187:drug effects* / Q000378:metabolism / Q000503:physiopathology; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism*; D007703:Peptidyl-Dipeptidase A / Q000378:metabolism; D008517:Phytotherapy; D028321:Plant Preparations / Q000494:pharmacology / Q000627:therapeutic use; D051381:Rats; D011918:Rats, Inbred SHR; D055314:Seed Storage Proteins / Q000494:pharmacology / Q000627:therapeutic use*; D030262:Soybean Proteins / Q000494:pharmacology / Q000627:therapeutic use*; D013025:Glycine max / Q000737:chemistry*; D017392:Thiobarbituric Acid Reactive Substances; D014280:Triglycerides / Q000097:blood
chemical_list: D052179:Antigens, Plant; D001786:Blood Glucose; D004044:Dietary Proteins; D005916:Globulins; D017127:Glycation End Products, Advanced; D007529:Isoflavones; D008565:Membrane Proteins; D028321:Plant Preparations; D055314:Seed Storage Proteins; D030262:Soybean Proteins; D017392:Thiobarbituric Acid Reactive Substances; D014280:Triglycerides; C013894:beta-conglycinin protein, Glycine max; C113267:nephrin; D000804:Angiotensin II; D007703:Peptidyl-Dipeptidase A