title: Screening and evaluation of anti-respiratory syncytial virus compounds in cultured cells.
abstract: Respiratory syncytial virus (RSV) is a highly contagious pathogen that infects mainly ciliated cells of respiratory epithelium and type 1 pneumocytes in the alveoli frequently causing serious respiratory disease in infants, elderly, and immunocompromised patients. At present, prevention/treatment of RSV infection is limited to the use of specific anti-RSV antibody or an aerosol formulation of ribavirin, a drug of suboptimal efficacy and low safety profile. There is an urgent need for development of novel anti-RSV drugs and virucides. Here we describe the cell culture-based methods used in our laboratory in identification of novel inhibitors of RSV including the P13 fusion inhibitor, and the PG545 virucide. Protocols for antiviral screening, evaluation of anti-RSV potency, and elucidation of mode of antiviral activity of test compounds are described.
authors.affiliation: Department of Clinical Virology, University of Gothenburg, Göteborg, Sweden.
authors.forename: Anna
authors.initials: A
authors.lastname: Lundin
authors.forename: Tomas
authors.initials: T
authors.lastname: Bergström
authors.forename: Edward
authors.initials: E
authors.lastname: Trybala
mesh_terms: D000998:Antiviral Agents / Q000494:pharmacology* / Q000633:toxicity; D018929:Cell Culture Techniques; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D003588:Cytopathogenic Effect, Viral / Q000187:drug effects; D024882:Drug Resistance, Viral / Q000235:genetics; D057166:High-Throughput Screening Assays; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods*; D018113:Respiratory Syncytial Virus, Human / Q000187:drug effects* / Q000235:genetics; D010948:Viral Plaque Assay
chemical_list: D000998:Antiviral Agents