title: Rapid assay for detecting gyrA and parC mutations associated with fluoroquinolone resistance in Enterobacteriaceae.
abstract: We developed a PCR-RFLP assay to detect mutations in the quinolone-resistance determining regions of gyrA and parC associated with fluoroquinolone resistance in Enterobacteriaceae. The assay detected mutations associated with reduced susceptibility to fluoroquinolones and may therefore serve as a specific, rapid, inexpensive, and simple testing alternative.
authors.affiliation: Department of Microbiology and Immunology, Teikyo University School of Medicine, 2-11-1 Kaga, Itabashi-ku, Tokyo 173-8605, Japan. nakano@med.teikyo-u.ac.jp
authors.forename: Ryuichi
authors.initials: R
authors.lastname: Nakano
authors.forename: Ryoichi
authors.initials: R
authors.lastname: Okamoto
authors.forename: Akiyo
authors.initials: A
authors.lastname: Nakano
authors.forename: Noriyuki
authors.initials: N
authors.lastname: Nagano
authors.forename: Michiko
authors.initials: M
authors.lastname: Abe
authors.forename: Shigeru
authors.initials: S
authors.lastname: Tansho-Nagakawa
authors.forename: Tsuneyuki
authors.initials: T
authors.lastname: Ubagai
authors.forename: Takane
authors.initials: T
authors.lastname: Kikuchi-Ueda
authors.forename: Osamu
authors.initials: O
authors.lastname: Koshio
authors.forename: Hirotoshi
authors.initials: H
authors.lastname: Kikuchi
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Ono
mesh_terms: D019943:Amino Acid Substitution / Q000235:genetics; D001483:Base Sequence; D027081:DNA Gyrase / Q000235:genetics*; D027101:DNA Topoisomerase IV / Q000235:genetics*; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D004755:Enterobacteriaceae / Q000187:drug effects / Q000235:genetics; D024841:Fluoroquinolones / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D016415:Sequence Alignment
chemical_list: D024841:Fluoroquinolones; D027101:DNA Topoisomerase IV; D027081:DNA Gyrase
keywords: Enterobacteriaceae; Fluoroquinolones; PCR-restriction fragment length polymorphism; Quinolone resistance-determining regions