title: Association of genetic polymorphisms in matrix metalloproteinase-9 and coronary artery disease in the Chinese Han population: a case-control study.
abstract: OBJECTIVE
  Matrix metalloproteinase-9 (MMP-9) plays an important role in inflammation and matrix degradation involved in atherosclerosis and plaque rupture. The T allele of rs3918242 has been reported to lead to a high promoter activity and associate with the extent of coronary artery disease (CAD). And some studies have reported that the G allele of rs17576 might be associated with CAD. The aim of this study was to assess the association between the polymorphisms of the MMP-9 gene and CAD in the Chinese Han population.
  
  
  METHODS
  This case-control study comprised 258 CAD cases and 153 controls from the Chinese Han Population. The genomic DNA of MMP-9 was isolated from whole blood. Polymerase chain reaction-based restriction fragment length polymorphism was used to determine the rs3918242 and rs17576 genotypes in the MMP-9 gene and the total serum levels of MMP-9 were measured using enzyme-linked immunosorbent assay in both case and control groups.
  
  
  RESULTS
  Analysis of MMP-9 gene polymorphisms showed that the frequencies of the T allele and CT+TT genotypes of rs3918242 were significantly higher in the case group than in the control group (p<0.05). However, the distribution of variant genotypes of rs17576 did not differ between the case and control groups (p>0.05). The total serum level of MMP-9 was significantly higher in the case group than in the control group (p<0.05). The subjects carrying T alleles in the CAD group had higher average serum MMP-9 levels compared with CC genotypes (p<0.05).
  
  
  CONCLUSIONS
  Our results suggest that the single-nucleotide polymorphism of rs3918242 in the MMP-9 gene is associated with CAD and high serum levels of MMP-9 are also associated with CAD in the Chinese Han population. Therefore, genetic variation of rs3918242 may participate in the development of CAD through influencing MMP-9 expression.
authors.affiliation: Department of Cardiology, First Hospital, Jilin University, Changchun, China.
authors.forename: Hai-di
authors.initials: HD
authors.lastname: Wu
authors.forename: Xiao
authors.initials: X
authors.lastname: Bai
authors.forename: Dong-mei
authors.initials: DM
authors.lastname: Chen
authors.forename: Hong-yan
authors.initials: HY
authors.lastname: Cao
authors.forename: Ling
authors.initials: L
authors.lastname: Qin
mesh_terms: D000368:Aged; D000483:Alleles; D044466:Asian People; D016022:Case-Control Studies; D002681:China / Q000453:epidemiology / Q000208:ethnology; D003324:Coronary Artery Disease / Q000097:blood / Q000201:enzymology / Q000453:epidemiology / Q000208:ethnology / Q000235:genetics*; D005260:Female; D015971:Gene Expression Regulation, Enzymologic / Q000235:genetics; D006801:Humans; D008297:Male; D020780:Matrix Metalloproteinase 9 / Q000096:biosynthesis / Q000235:genetics*; D008875:Middle Aged; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide
chemical_list: C579270:MMP9 protein, human; D020780:Matrix Metalloproteinase 9