title: In vitro propagation of Stemona hutanguriana W.Chuakul, an endangered medicinal plant.
abstract: In vitro propagation of Stemona hutanguriana W.Chuakul, an endangered species of Stemonaceae, was established. Nodal and internodal explants were cultured on Murashige and Skoog (MS) medium supplemented with thidiazuron (TDZ) alone or in combination with 1-naphthaleneacetic acid (NAA). When cultured on MS medium supplemented with 18.16 μM TDZ with 0.54 μM NAA for 8 weeks and then on MS medium without plant growth regulator (PGR) for 8 weeks, nodal explants demonstrated a responding frequency of 91.67 % and a shoot regeneration rate of 5.46 shoots/responding explant. Furthermore, internodal explants demonstrated a responding frequency of 17.17 % with 11.17 shoots/responding explant. The regenerated shoots were rooted using a two-step protocol by culturing for 4 weeks on MS medium supplemented with various concentrations of NAA and Indole-3-butyric acid (IBA) and 8 weeks on MS medium without PGR. When 5.40 μM NAA was used as a PGR, the maximum root induction rate was 69.45 %. Regenerated plantlets were successfully transferred to soil.
authors.affiliation: Department of Pharmaceutical Botany, Faculty of Pharmacy, Mahidol University, 447 Sri-ayudthaya Road, Bangkok, 10400 Thailand.
authors.forename: Sompop
authors.initials: S
authors.lastname: Prathanturarug
authors.forename: Rungthipwan
authors.initials: R
authors.lastname: Pheakkoet
authors.forename: Thaya
authors.initials: T
authors.lastname: Jenjittikul
authors.forename: Wongsatit
authors.initials: W
authors.lastname: Chuakul
authors.forename: Promchit
authors.initials: P
authors.lastname: Saralamp
keywords: Conservation; Medicinal plant; Stemona hutanguriana; Stemonaceae; Tissue culture