title: Sasa quelpaertensis Nakai extract and its constituent p-coumaric acid inhibit adipogenesis in 3T3-L1 cells through activation of the AMPK pathway.
abstract: In this study, we investigated the effects of Sasa quelpaertensis Nakai extract (SQE) and its main constituent, p-coumaric acid, on adipogenesis in 3T3-L1 cells. SQE markedly inhibited adipogenesis by downregulating the expression of CCAAT/enhancer-binding protein α (C/EBPα), peroxisome proliferator-activated receptor γ (PPARγ), sterol regulatory element-binding protein-1c (SREBP-1c), and aP2. It also decreased the expression of fatty acid synthase (FAS) and adiponectin mRNAs in differentiating adipocytes. SQE increased AMP-activated protein kinase (AMPK) and acetyl-CoA carboxylase (ACC) phosphorylation during the early phase of MDI-induced differentiation, suggesting that SQE exerted its anti-adipogenic effect via AMPK activation at an early stage of the differentiation process. p-Coumaric acid suppressed adipogenesis by attenuating the expression of C/EBPα, PPARγ, and SREBP-1c during the late phase of MDI-induced differentiation. In addition, p-coumaric acid increased the phosphorylation of AMPK and ACC, and the expression of carnitine palmitoyl transferase-1 (CPT-1) mRNA, in fully differentiated adipocytes, indicating that it promotes fatty acid β-oxidation via AMPK signaling. Taken together, our data suggest that SQE and p-coumaric acid might have the anti-obesitic effects via AMPK pathway in 3T3-L1 cells.
authors.affiliation: Department of Biology, Jeju National University, Jejusi, Jeju Special Self-Governing Province, Republic of Korea.
authors.forename: Seung-Woo
authors.initials: SW
authors.lastname: Kang
authors.forename: Seong-Il
authors.initials: SI
authors.lastname: Kang
authors.forename: Hye-Sun
authors.initials: HS
authors.lastname: Shin
authors.forename: Seon-A
authors.initials: SA
authors.lastname: Yoon
authors.forename: Jeong-Hwan
authors.initials: JH
authors.lastname: Kim
authors.forename: Hee-Chul
authors.initials: HC
authors.lastname: Ko
authors.forename: Se-Jae
authors.initials: SJ
authors.lastname: Kim
mesh_terms: D041721:3T3-L1 Cells; D055372:AMP-Activated Protein Kinases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D052438:Adipocytes, White / Q000166:cytology / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D050156:Adipogenesis / Q000187:drug effects*; D000818:Animals; D019440:Anti-Obesity Agents / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D003373:Coumaric Acids / Q000032:analysis / Q000302:isolation & purification / Q000494:pharmacology*; D015536:Down-Regulation / Q000187:drug effects; D004789:Enzyme Activation / Q000187:drug effects; D007700:Kinetics; D050356:Lipid Metabolism / Q000187:drug effects; D051379:Mice; D010766:Phosphorylation / Q000187:drug effects; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D011422:Propionates; D011499:Protein Processing, Post-Translational / Q000187:drug effects; D012333:RNA, Messenger / Q000378:metabolism; D056910:Republic of Korea; D031787:Sasa / Q000737:chemistry*; D015398:Signal Transduction / Q000187:drug effects*
chemical_list: D019440:Anti-Obesity Agents; D003373:Coumaric Acids; D010936:Plant Extracts; D011422:Propionates; D012333:RNA, Messenger; D055372:AMP-Activated Protein Kinases; C495469:p-coumaric acid
keywords: 3T3-L1 adipocyte; AMP – activated protein kinase; Adipogenesis; Sasa quelpaertensis; p-Coumaric acid