title: Evaluation of cycloserine-cefoxitin fructose agar (CCFA), CCFA with horse blood and taurocholate, and cycloserine-cefoxitin mannitol broth with taurocholate and lysozyme for recovery of Clostridium difficile isolates from fecal samples.
abstract: Cycloserine-cefoxitin fructose agar (CCFA), CCFA with horse blood and taurocholate (CCFA-HT), and cycloserine-cefoxitin mannitol broth with taurocholate and lysozyme (CCMB-TAL) were compared for recovery of Clostridium difficile from 120 stool specimens. Compared to CCFA, CCFA-HT enhanced C. difficile growth and improved recovery by 4%. In a separate study, 9% (8/91) of stool samples previously C. difficile negative on plate medium were C. difficile positive when cultured in CCMB-TAL.
authors.affiliation: RM Alden Research Laboratory, Culver City, California, USA.
authors.forename: Kerin L
authors.initials: KL
authors.lastname: Tyrrell
authors.forename: Diane M
authors.initials: DM
authors.lastname: Citron
authors.forename: Eliza S
authors.initials: ES
authors.lastname: Leoncio
authors.forename: C Vreni
authors.initials: CV
authors.lastname: Merriam
authors.forename: Ellie J C
authors.initials: EJ
authors.lastname: Goldstein
mesh_terms: D000362:Agar; D000818:Animals; D000890:Anti-Infective Agents / Q000378:metabolism; D002440:Cefoxitin / Q000378:metabolism; D016360:Clostridioides difficile / Q000302:isolation & purification*; D003015:Clostridium Infections / Q000175:diagnosis* / Q000382:microbiology*; D003470:Culture Media / Q000737:chemistry*; D003523:Cycloserine / Q000378:metabolism; D004912:Erythrocytes / Q000378:metabolism; D005243:Feces / Q000382:microbiology*; D005632:Fructose / Q000378:metabolism; D006736:Horses; D006801:Humans; D008353:Mannitol / Q000378:metabolism; D009113:Muramidase / Q000378:metabolism; D013656:Taurocholic Acid / Q000378:metabolism
chemical_list: D000890:Anti-Infective Agents; D003470:Culture Media; D005632:Fructose; D008353:Mannitol; D013656:Taurocholic Acid; D002440:Cefoxitin; D000362:Agar; D003523:Cycloserine; D009113:Muramidase