title: Bioengineered trachea using autologous chondrocytes for regeneration of tracheal cartilage in a rabbit model.
abstract: OBJECTIVE
  In this study, a bioengineered trachea composed of autologous chondrocytes was developed, and its effect on cartilaginous regeneration was evaluated by implantation into tracheal defects in rabbits.
  
  
  METHODS
  Prospective controlled trial in an animal model.
  
  
  METHODS
  The tracheal prosthesis used in this study was composed of polypropylene (the frame) and collagen sponge (the scaffold). Chondrocytes were harvested from the costal cartilage of rabbits and seeded into the tracheal prosthesis. The bioengineered trachea, consisting of the tracheal prosthesis with chondrocytes, was implanted into surgically created tracheal defects of rabbits in the bioengineered group, and a tracheal prosthesis without chondrocytes was implanted in the control group.
  
  
  RESULTS
  After implantation, the presence of regenerated cartilage was observed in the bioengineered trachea but not in the tracheal prosthesis without chondrocytes.
  
  
  CONCLUSIONS
  It was confirmed in this study that the implanted chondrocytes proliferated in an appropriate portion of the tracheal defect and that the partially resected tracheal cartilage was repaired with regenerated cartilaginous tissue into a ring-shaped form as a whole. These results demonstrate the feasibility of cartilaginous regeneration using a bioengineered trachea with autologous chondrocytes.
authors.affiliation: Department of Otolaryngology, School of Medicine, Fukushima Medical University, Fukushima.
authors.forename: Mika
authors.initials: M
authors.lastname: Nomoto
authors.forename: Yukio
authors.initials: Y
authors.lastname: Nomoto
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Tada
authors.forename: Akiko
authors.initials: A
authors.lastname: Tani
authors.forename: Koshi
authors.initials: K
authors.lastname: Otsuki
authors.forename: Ryo
authors.initials: R
authors.lastname: Suzuki
authors.forename: Tatsuo
authors.initials: T
authors.lastname: Nakamura
authors.forename: Koichi
authors.initials: K
authors.lastname: Omori
mesh_terms: D000818:Animals; D002356:Cartilage / Q000166:cytology*; D019902:Chondrocytes / Q000166:cytology / Q000637:transplantation*; D004195:Disease Models, Animal; D008297:Male; D019736:Prostheses and Implants; D011474:Prosthesis Design; D011817:Rabbits; D011897:Random Allocation; D012038:Regeneration; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds; D014132:Trachea / Q000502:physiology / Q000601:surgery*; D014139:Tracheostomy / Q000379:methods; D014182:Transplantation, Autologous
keywords: Chondrocyte; cartilage; regeneration; trachea