title: A pyrene-based highly selective turn-on fluorescent chemosensor for iron(III) ions and its application in living cell imaging.
abstract: A new pyrene-based chemosensor (1) exhibits excellent selectivity for Fe(3+) ions over a wide range of tested metal ions Ag(+), Ca(2+), Cd(2+), Co(2+), Cu(2+), Fe(2+), Hg(2+), K(+), Mg(2+), Mn(2+), Ni(2+), Pb(2+), and Zn(2+). The binding of Fe(3+) to chemosensor 1 produces an emission band at 507 nm due to the formation of a Py-Py* excimer that is induced by Fe(3+)-binding. The binding ratio of 1-Fe(3+) was determined to be 1:1 from a Job plot. The association constant of 1-Fe(3+) complexes was found to be 1.27 × 10(4) M(-1) from a Benesi-Hildebrand plot. In addition, fluorescence microscopy experiments show that 1 can be used as a fluorescent probe for detecting Fe(3+) in living cells.
authors.affiliation: Department of Applied Chemistry, National Chiao Tung University, Hsinchu, Taiwan, 300, Republic of China.
authors.forename: Peter Kun
authors.initials: PK
authors.lastname: Chung
authors.forename: Shi-Rong
authors.initials: SR
authors.lastname: Liu
authors.forename: Hsuan-Fu
authors.initials: HF
authors.lastname: Wang
authors.forename: Shu-Pao
authors.initials: SP
authors.lastname: Wu
mesh_terms: D005290:Ferric Compounds / Q000032:analysis*; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D007477:Ions / Q000032:analysis; D015394:Molecular Structure; D011721:Pyrenes / Q000138:chemical synthesis / Q000737:chemistry*; D011789:Quantum Theory; D013050:Spectrometry, Fluorescence / Q000295:instrumentation; D014407:Tumor Cells, Cultured
chemical_list: D005290:Ferric Compounds; D005456:Fluorescent Dyes; D007477:Ions; D011721:Pyrenes; C030984:pyrene