title: A chemoenzymatic route to synthesize unnatural sugar nucleotides using a novel N-acetylglucosamine-1-phosphate pyrophosphorylase from Camphylobacter jejuni NCTC 11168.
abstract: A novel N-acetylglucosamine-1-phosphate pyrophosphorylase was identified from Campylobacter jejuni NCTC 11168. An unprecedented degree of substrate promiscuity has been revealed by systematic studies on its substrate specificities towards sugar-1-P and NTP. The yields of the synthetic reaction of seven kinds of sugar nucleotides catalyzed by the enzyme were up to 60%. In addition, the yields of the other nine were around 20%. With this enzyme, three novel sugar nucleotide analogs were synthesized on a preparative scale and well characterized.
authors.affiliation: National Glycoengineering Research Center, Shandong University, Jinan, Shandong 250100, People's Republic of China.
authors.forename: Junqiang
authors.initials: J
authors.lastname: Fang
authors.forename: Mengyang
authors.initials: M
authors.lastname: Xue
authors.forename: Guofeng
authors.initials: G
authors.lastname: Gu
authors.forename: Xian-wei
authors.initials: XW
authors.lastname: Liu
authors.forename: Peng George
authors.initials: PG
authors.lastname: Wang
mesh_terms: D000117:Acetylglucosamine / Q000031:analogs & derivatives / Q000737:chemistry; D016123:Campylobacter jejuni / Q000201:enzymology*; D002241:Carbohydrates / Q000737:chemistry*; D009711:Nucleotides / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism; D009713:Nucleotidyltransferases / Q000737:chemistry / Q000378:metabolism*; D013329:Structure-Activity Relationship; D013379:Substrate Specificity
chemical_list: D002241:Carbohydrates; D009711:Nucleotides; C026146:N-acetylglucosamine-1-phosphate; D009713:Nucleotidyltransferases; D000117:Acetylglucosamine