title: Suppression of adipogenic differentiation by muscle cell-induced decrease in genes related to lipogenesis in muscle and fat co-culture system.
abstract: Intercellular signalling communication between adipose and muscle tissue has been investigated. To test the effect of muscle cells on adipogenic gene expression, we utilised an in vitro co-culture system, in which fat (3T3-L1) and muscle (L-6) cells were physically separated but chemically exposed each other via insert with 0.4 µm porous membrane. When 3T3-L1 and L-6 cells reached at 80 and 40% confluence, respectively in separate wells, L-6 cells grown in insert were transferred onto 6-well plates where 3T3-L1 cells were being grown. When both cells were fully differentiated in co-culture plates, morphology of 3T3-L1 was examined by staining with Oil-red-O. Activity of glycerol-3-phosphate dehydrogenase (GPDH) and adipogenic gene expression including lipoprotein lipase (LPL), adipsin, GPDH, peroxisome proliferator-activated receptor-γ (PPARγ) and CCAAT/enhancer binding protein (C/EBPα) were analysed. The presence of muscle cells during preadipocyte differentiation inhibited (P < 0.05) lipogenesis by suppressing lipogenic gene expression including LPL, adipsin and GPDH. Furthermore, GPDH activity was also decreased (P < 0.05) in 3T3-L1 cells by the presence of L-6 cells. These results suggest that presence of muscle cells suppresses adipogenic differentiation by inhibiting the adipogenic gene expression and GPDH activity in the muscle and fat cell co-culture system.
authors.affiliation: National Institute of Animal Science, RDA, Suwon, 441-706, Korea.
authors.forename: Sungkwon
authors.initials: S
authors.lastname: Park
authors.forename: Kyunghoon
authors.initials: K
authors.lastname: Baek
authors.forename: Changbon
authors.initials: C
authors.lastname: Choi
mesh_terms: D041721:3T3-L1 Cells; D050156:Adipogenesis / Q000235:genetics*; D000818:Animals; D022762:CCAAT-Enhancer-Binding Proteins / Q000235:genetics / Q000378:metabolism; D002450:Cell Communication / Q000235:genetics*; D002454:Cell Differentiation; D018920:Coculture Techniques; D011416:Complement Factor D / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D015194:Diffusion Chambers, Culture; D005786:Gene Expression Regulation / Q000235:genetics*; D005993:Glycerolphosphate Dehydrogenase / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D050155:Lipogenesis / Q000235:genetics*; D008071:Lipoprotein Lipase / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D051379:Mice; D018485:Muscle Fibers, Skeletal / Q000166:cytology / Q000378:metabolism*; D047495:PPAR gamma / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction
chemical_list: D022762:CCAAT-Enhancer-Binding Proteins; C530718:CEBPA protein, mouse; D047495:PPAR gamma; D005993:Glycerolphosphate Dehydrogenase; D008071:Lipoprotein Lipase; D011416:Complement Factor D; C493491:complement factor D, mouse
keywords: 3T3-L1; GPDH; L6; cell differentiation; gene expression