title: Inhibitory effects of syk transfection on lung cancer cell invasion.
abstract: OBJECTIVE
  Spleen tyrosine kinase (Syk) is closely related to tumor invasion and metastasis, and has been shown to have potential inhibitory effects in tumors. In this study, we constructed a eukaryotic expression vector for Syk and analyzed its effects on invasive ability of the A549 non-small cell lung cancer cell line in vitro.
  
  
  METHODS
  A fragment of Syk was obtained by RT-PCR from human lung cancer cells and cloned into the expression vector pLNCXSyk. After restriction endonuclease digestion, PCR and DNA sequencing confirmation, the recombinant Syk expression plasmid was transfected into A549 human lung cancer cells using lipofectamine protocols. After selection, the cells stably expressed Syk. Detection of Syk expression of the cells by RT-PCR, and invasive ability were examined.
  
  
  RESULTS
  The eukaryotic expression plamid pLNCXSyk was constructed and expressed stably in the A549 human lung cancer cells. The RT-PCR results showed that Syk mRNA expression was upregulated significantly (P<0.05). Lower invasion through a basal membrane were apparent after transfection (P<0.05).
  
  
  CONCLUSIONS
  A eukaryotic expression plasmid to cause Syk expression in lung cancer cells can obviously inhibit their invasive ability in vitro.
authors.affiliation: Department of Thoracic, The Second Hospital of Shandong University, Jinan, China. pechuliang@yahoo.com.cn
authors.forename: Chuan-Liang
authors.initials: CL
authors.lastname: Peng
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhang
authors.forename: Qi-Feng
authors.initials: QF
authors.lastname: Sun
authors.forename: Yun-Peng
authors.initials: YP
authors.lastname: Zhao
authors.forename: Ying-Tao
authors.initials: YT
authors.lastname: Hao
authors.forename: Xiao-Gang
authors.initials: XG
authors.lastname: Zhao
authors.forename: Bo
authors.initials: B
authors.lastname: Cong
mesh_terms: D002289:Carcinoma, Non-Small-Cell Lung / Q000201:enzymology / Q000235:genetics / Q000473:pathology*; D002465:Cell Movement; D049109:Cell Proliferation; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism*; D008175:Lung Neoplasms / Q000201:enzymology / Q000235:genetics / Q000473:pathology*; D009361:Neoplasm Invasiveness; D011505:Protein-Tyrosine Kinases / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D000072377:Syk Kinase; D014407:Tumor Cells, Cultured
chemical_list: D047908:Intracellular Signaling Peptides and Proteins; D012333:RNA, Messenger; D011505:Protein-Tyrosine Kinases; C000606896:SYK protein, human; D000072377:Syk Kinase