title: [Effects of DiBP on the cAMP/PKA-CREB-BDNF signaling pathway of hippocampus in mice].
abstract: OBJECTIVE
  To explore the effects of diisobutyl phthalate (DiBP) on the cAMP/PKA-CREB signaling pathway of hippocampus in mice.
  
  
  METHODS
  Accommodating 30 KunMing mice to the animal house for 3 days, then dividing the mice into 5 groups according to their weights. That is, one control group and four experimental groups (I group, 50 mg/kg BW; II group, 250 mg/kg BW; Ill group, 500 mg/kg BW; IV group, 1000 mg/kg BW). The mice were fed with the corn oil in control group, and the other groups were fed with the related dose of DiBP mixture by gavages last for 8 weeks. At the end of experimental time, the mice were killed, and the tissue samples of hippocampus were taken immediately. The content of cAMP was determined by ELISA, and p-CREB, P-PKA C were measured by western blotting, while mRNA expressions including CREB, BDNF, c-fos and c-jun were checked by RT-PCR.
  
  
  RESULTS
  The cAMP content and the p-PKA C protein of hippocampus in IV group was significantly less than control group (P<0.05) and compared with control group, the p-CREB protein of hippocampus in 1I group decreased (P<0.05), while the relative level of CREB, BDNF, c-fos and c-jun mRNA were down-regulated in all experimental groups.
  
  
  CONCLUSIONS
  The abnormal changes of expression of the signal molecules composing the cAMP/PKA-CREB signaling pathway were observed in the hippocampus of DiBP exposure mice. This might be one of the possible mechanisms of DiBP induced cognitive impairment.
authors.affiliation: National Center for Food Safety Risk Assessment, Beijing 100021, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Ma
authors.forename: Xiaodan
authors.initials: X
authors.lastname: Wang
authors.forename: Peng
authors.initials: P
authors.lastname: Gao
authors.forename: Haibin
authors.initials: H
authors.lastname: Xu
mesh_terms: D000818:Animals; D019208:Brain-Derived Neurotrophic Factor / Q000378:metabolism*; D017362:Cyclic AMP Response Element-Binding Protein / Q000378:metabolism*; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism*; D003993:Dibutyl Phthalate / Q000031:analogs & derivatives* / Q000494:pharmacology; D006624:Hippocampus / Q000378:metabolism*; D008297:Male; D051379:Mice; D015398:Signal Transduction / Q000187:drug effects
chemical_list: D019208:Brain-Derived Neurotrophic Factor; D017362:Cyclic AMP Response Element-Binding Protein; D003993:Dibutyl Phthalate; D017868:Cyclic AMP-Dependent Protein Kinases; C025605:diisobutyl phthalate