title: RNA isolation from mammalian samples.
abstract: RNA can be extracted from cultured cells, peripheral blood, bone marrow, plasma, serum, body fluids, and fresh or frozen tissues. RNA can also be extracted from formalin-fixed paraffin-embedded (FFPE) tissues. Methods for RNA extraction can be divided into three groups: phenol/chloroform extraction, silica spin-column absorption, and isopycnic gradient centrifugation. Two different RNA isolation procedures are described in this unit. The first basic protocol describes a one-step isolation involving monophasic lysis with guanidine isothiocyanate and phenol followed by chloroform extraction. The second basic protocol describes a silica-column separation method for RNA isolation.
authors.affiliation: Department of Pathology, University of Alabama at Birmingham, Birmingham, AL, USA.
authors.forename: Ximeng
authors.initials: X
authors.lastname: Liu
authors.forename: Shuko
authors.initials: S
authors.lastname: Harada
mesh_terms: D000818:Animals; D006801:Humans; D008322:Mammals; D008967:Molecular Biology / Q000379:methods*; D012313:RNA / Q000302:isolation & purification*; D013048:Specimen Handling / Q000379:methods*
chemical_list: D012313:RNA