title: Prognostic value of miR-155 in individuals with monoclonal B-cell lymphocytosis and patients with B chronic lymphocytic leukemia.
abstract: Noncoding RNAs play a pivotal role in the pathogenesis of chronic lymphocytic leukemia (CLL). We hypothesized that microRNAs (miRs) are involved in the transition from monoclonal B-cell lymphocytosis (MBL) to CLL and tested miR-15a/16-1 cluster, miR-21, and miR-155 expression in purified B cells of normal individuals, individuals with MBL, and patients with CLL. When we analyzed 224 samples from 2 independent training and validation cohorts, we found that miR-155 was overexpressed in B cells from individuals with MBL, and even more so in B cells from patients with CLL, when compared with B cells from normal individuals. Furthermore, we were able to identify miR-155 in circulating microvesicles from both individuals with MBL and patients with CLL. Next, to examine the prognostic role of miR-155, we measured its expression level in plasma samples collected before treatment initiation in 228 patients with CLL. We found significantly higher miR-155 expression levels in patients who failed to achieve a complete response compared with those who experienced complete response. Our findings support the use of cellular and plasma levels of miR-155 as biomarkers for the risk of progression in individuals with MBL, as well as to identify patients with CLL who may not respond well to therapy.
authors.affiliation: Department of Leukemia, The University of Texas MD Anderson Cancer Center, Houston, TX;
authors.forename: Alessandra
authors.initials: A
authors.lastname: Ferrajoli
authors.forename: Tait D
authors.initials: TD
authors.lastname: Shanafelt
authors.forename: Cristina
authors.initials: C
authors.lastname: Ivan
authors.forename: Masayoshi
authors.initials: M
authors.lastname: Shimizu
authors.forename: Kari G
authors.initials: KG
authors.lastname: Rabe
authors.forename: Nazila
authors.initials: N
authors.lastname: Nouraee
authors.forename: Mariko
authors.initials: M
authors.lastname: Ikuo
authors.forename: Asish K
authors.initials: AK
authors.lastname: Ghosh
authors.forename: Susan
authors.initials: S
authors.lastname: Lerner
authors.forename: Laura Z
authors.initials: LZ
authors.lastname: Rassenti
authors.forename: Lianchun
authors.initials: L
authors.lastname: Xiao
authors.forename: Jianhua
authors.initials: J
authors.lastname: Hu
authors.forename: James M
authors.initials: JM
authors.lastname: Reuben
authors.forename: Steliana
authors.initials: S
authors.lastname: Calin
authors.forename: M James
authors.initials: MJ
authors.lastname: You
authors.forename: John T
authors.initials: JT
authors.lastname: Manning
authors.forename: William G
authors.initials: WG
authors.lastname: Wierda
authors.forename: Zeev
authors.initials: Z
authors.lastname: Estrov
authors.forename: Susan
authors.initials: S
authors.lastname: O'Brien
authors.forename: Thomas J
authors.initials: TJ
authors.lastname: Kipps
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Keating
authors.forename: Neil E
authors.initials: NE
authors.lastname: Kay
authors.forename: George A
authors.initials: GA
authors.lastname: Calin
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001402:B-Lymphocytes / Q000378:metabolism* / Q000473:pathology; D014408:Biomarkers, Tumor / Q000097:blood / Q000235:genetics; D015331:Cohort Studies; D018450:Disease Progression; D005260:Female; D015870:Gene Expression; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D053208:Kaplan-Meier Estimate; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000097:blood / Q000188:drug therapy / Q000235:genetics*; D008218:Lymphocytosis / Q000097:blood / Q000188:drug therapy / Q000235:genetics*; D008297:Male; D035683:MicroRNAs / Q000097:blood / Q000235:genetics*; D055806:Microvessels / Q000378:metabolism; D008875:Middle Aged; D015999:Multivariate Analysis; D011379:Prognosis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016896:Treatment Outcome
chemical_list: D014408:Biomarkers, Tumor; C524942:MIRN155 microRNA, human; D035683:MicroRNAs