title: Synthesis and characterization of a pH-sensitive conjugate of isoniazid with Fe3O4@SiO2 magnetic nanoparticles.
abstract: The Letter describes the preparation and characterization of a conjugate of isoniazid (INH) with magnetic nanoparticles Fe3O4@SiO2 115±60 nm in size. The INH molecules were attached to the surface of nanoparticles by a covalent pH-sensitive amidine bond. The conjugate was characterized by X-ray diffraction, SEM, dynamic light scattering, IR spectroscopy and microanalysis. The conjugate released isoniazid under in vitro conditions (pH=4; 37 °C; t1/2≈115 s). In addition, the cytotoxicity of the Fe3O4@SiO2-INH conjugate was evaluated in SK-BR-3 cells using the xCELLigence system.
authors.affiliation: Institute of Organic Chemistry and Technology, Faculty of Chemical Technology, University of Pardubice, Studentská 573, 532 10 Pardubice, Czech Republic. milos.sedlak@upce.cz
authors.forename: Miloš
authors.initials: M
authors.lastname: Sedlák
authors.forename: Dattatry Shivajirao
authors.initials: DS
authors.lastname: Bhosale
authors.forename: Ludvík
authors.initials: L
authors.lastname: Beneš
authors.forename: Jiří
authors.initials: J
authors.lastname: Palarčík
authors.forename: Andrea
authors.initials: A
authors.lastname: Kalendová
authors.forename: Karel
authors.initials: K
authors.lastname: Královec
authors.forename: Aleš
authors.initials: A
authors.lastname: Imramovský
mesh_terms: D000900:Anti-Bacterial Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000633:toxicity; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D005290:Ferric Compounds / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007538:Isoniazid / Q000138:chemical synthesis* / Q000737:chemistry / Q000633:toxicity*; D008280:Magnetics; D008855:Microscopy, Electron, Scanning; D053758:Nanoparticles / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry*; D014961:X-Ray Diffraction
chemical_list: D000900:Anti-Bacterial Agents; D005290:Ferric Compounds; C000499:ferric oxide; D012822:Silicon Dioxide; D007538:Isoniazid
keywords: Isoniazid; Magnetic nanoparticle; Prodrug; Tuberculostatic agent; pH-sensitivity