title: Native trees of the Northeast Argentine: natural hosts of the Cryptococcus neoformans-Cryptococcus gattii species complex.
abstract: BACKGROUND
  In Argentina, information about epidemiology and environmental distribution of Cryptococcus is scarce. The city of Resistencia borders with Brazil and Paraguay where this fungus is endemic. All these supported the need to investigate the ecology of the genus and the epidemiology of cryptococcosis in this area.
  
  
  OBJECTIVE
  The aim was to investigate the presence of species of Cryptococcus neoformans-Cryptococcus gattii complex and their genotypes in trees of the city of Resistencia.
  
  
  METHODS
  One hundred and five trees were sampled by swabbing technique. The isolates were identified using conventional and commercial methods and genotyped by PCR-RFLP (Restriction Fragment Length Polymorphism).
  
  
  RESULTS
  Cryptococcus was found in 7 out of the total trees. 6 out of 7 Cryptococcus isolates were identified as C. neoformans and one as C. gattii. C. gattii was isolated from Grevillea robusta. C. neoformans strains were isolated from Tabebuia avellanedae and Peltophorum dubium. Genotyping showed that all C. neoformans belonged to the VNI type and C. gattii belonged to the VGI type.
  
  
  CONCLUSIONS
  This represents the first study on the ecology of Cryptococcus spp. associated to trees from northeastern Argentina, and the first report describing Grevillea robusta as a host of members of this fungal genus. Another finding is the isolation of C. neoformans from Tabebuia avellanedae and Peltophorum dubium, both tree species native to northeastern Argentina.
authors.affiliation: Departamento de Micología, Instituto de Medicina Regional, Universidad Nacional del Nordeste, Resistencia, Argentina. Electronic address: memiliacattana@yahoo.com.ar.
authors.forename: Maria Emilia
authors.initials: ME
authors.lastname: Cattana
authors.affiliation: Departamento de Micología, Instituto de Medicina Regional, Universidad Nacional del Nordeste, Resistencia, Argentina.
authors.forename: María de Los Ángeles
authors.initials: Mde L
authors.lastname: Sosa
authors.affiliation: Departamento de Micología, Instituto de Medicina Regional, Universidad Nacional del Nordeste, Resistencia, Argentina.
authors.forename: Mariana
authors.initials: M
authors.lastname: Fernández
authors.affiliation: Departamento de Micología, Instituto de Medicina Regional, Universidad Nacional del Nordeste, Resistencia, Argentina.
authors.forename: Florencia
authors.initials: F
authors.lastname: Rojas
authors.affiliation: Departamento de Micología, Instituto de Medicina Regional, Universidad Nacional del Nordeste, Resistencia, Argentina.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Mangiaterra
authors.affiliation: Departamento de Micología, Instituto de Medicina Regional, Universidad Nacional del Nordeste, Resistencia, Argentina.
authors.forename: Gustavo
authors.initials: G
authors.lastname: Giusiano
mesh_terms: D001118:Argentina; D056285:Cryptococcus gattii / Q000235:genetics / Q000302:isolation & purification*; D003455:Cryptococcus neoformans / Q000235:genetics / Q000302:isolation & purification*; D005838:Genotype; D014197:Trees / Q000382:microbiology*
keywords: Cryptococcus gattii; Ecological niche; Genotipo; Genotype; Grevillea robusta; Nicho ecológico; Peltophorum dubium; Tabebuia avellanedae