title: Genetic diversity among Trypanosoma (Duttonella) vivax strains from Zambia and Ghana, based on cathepsin L-like gene.
abstract: Understanding the evolutionary relationships of Trypanosoma (Duttonella) vivax genotypes between West Africa and Southern Africa can provide information on the epidemiology and control of trypanosomosis. Cattle blood samples from Zambia and Ghana were screened for T. vivax infection using specie-specific PCR and sequencing analysis. Substantial polymorphism was obtained from phylogenetic analysis of sequences of cathepsin L-like catalytic domains. T. vivax from Ghana clustered together with West African and South American sequences, while T. vivax from Zambia formed one distinct clade and clustered with East African and Southern African sequences. This study suggests existence of distinct genetic diversity between T. vivax genotypes from West Africa and Zambia as per their geographical origins.
authors.affiliation: Division of Collaboration and Education, Research Center for Zoonosis Control, Hokkaido University, Kita 20, Nishi 10, Kita-ku, Sapporo, Hokkaido 001-0020, Japan - National Livestock Resources Research Institute (NaLIRRI), P.O. Box 96 Tororo, Uganda.
authors.forename: Jesca
authors.initials: J
authors.lastname: Nakayima
authors.forename: Ryo
authors.initials: R
authors.lastname: Nakao
authors.forename: Andy
authors.initials: A
authors.lastname: Alhassan
authors.forename: Kyoko
authors.initials: K
authors.lastname: Hayashida
authors.forename: Boniface
authors.initials: B
authors.lastname: Namangala
authors.forename: Charles
authors.initials: C
authors.lastname: Mahama
authors.forename: Kofi
authors.initials: K
authors.lastname: Afakye
authors.forename: Chihiro
authors.initials: C
authors.lastname: Sugimoto
mesh_terms: D000818:Animals; D001483:Base Sequence; D056668:Cathepsin L / Q000235:genetics*; D002417:Cattle; D002418:Cattle Diseases / Q000469:parasitology*; D016000:Cluster Analysis; D016054:DNA, Protozoan / Q000737:chemistry; D014644:Genetic Variation; D005838:Genotype; D005869:Ghana; D008969:Molecular Sequence Data; D010802:Phylogeny; D016689:Trypanosoma vivax / Q000145:classification / Q000235:genetics*; D014353:Trypanosomiasis, African / Q000469:parasitology / Q000662:veterinary*; D015024:Zambia
chemical_list: D016054:DNA, Protozoan; D056668:Cathepsin L