title: Encapsulation in alginate and alginate coated-chitosan improved the survival of newly probiotic in oxgall and gastric juice.
abstract: This study was undertaken to develop an optimum composition model for the microencapsulation of a newly probiotic on sodium alginate using response surface methodology. The individual and interactive effects of three independent variables, namely sodium alginate concentration, biomass concentration, and hardening time, were investigated using Box-Behnken design experiments. A second ordered polynomial model was fitted and optimum conditions were estimated. The optimal conditions identified were 2% for sodium alginate, 10(10)UFC/ml for biomass, and 30 min for hardening time. The experimental value obtained for immobilized cells under these conditions was about 80.98%, which was in close agreement with the predicted value of 82.6%. Viability of microspheres (96%) was enhanced with chitosan as coating materials. The survival rates of free and microencapsulated Lactobacillus plantarum TN8 during exposure to artificial gastrointestinal conditions were compared. The results revealed that the encapsulated cells exhibited significantly higher resistances to artificial intestinal juice (AIJ) and artificial gastric juice (AGJ). Microencapsulation was also noted to effectively protect the strain from heating at 65 °C and refrigerating at 4 °C. Taken together, the findings indicated that microencapsulation conferred important protective effects to L. plantarum against the gastrointestinal conditions encountered during the transit of food.
authors.affiliation: Laboratory of Microorganisms and Biomolecules (LMB), Centre of Biotechnology of Sfax, University of Sfax, Road of Sidi Mansour Km 6, P.O. Box 1177, Sfax 3018, Tunisia.
authors.forename: Imen
authors.initials: I
authors.lastname: Trabelsi
authors.forename: Wacim
authors.initials: W
authors.lastname: Bejar
authors.forename: Dorra
authors.initials: D
authors.lastname: Ayadi
authors.forename: Hichem
authors.initials: H
authors.lastname: Chouayekh
authors.forename: Radhouane
authors.initials: R
authors.lastname: Kammoun
authors.forename: Samir
authors.initials: S
authors.lastname: Bejar
authors.forename: Riadh
authors.initials: R
authors.lastname: Ben Salah
mesh_terms: D000464:Alginates / Q000737:chemistry*; D001647:Bile Acids and Salts; D048271:Chitosan / Q000737:chemistry*; D004339:Drug Compounding / Q000379:methods; D005750:Gastric Juice / Q000382:microbiology*; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D048191:Lactiplantibacillus plantarum / Q000737:chemistry / Q000254:growth & development; D019936:Probiotics / Q000737:chemistry*; D013696:Temperature
chemical_list: D000464:Alginates; D001647:Bile Acids and Salts; D006603:Hexuronic Acids; D020723:Glucuronic Acid; D048271:Chitosan
keywords: Alginate; Chitosan; Microencapsulation