title: [The application of monoclonal peroxidase conjugates to identify comma bacillus of serum groups 01 and 0139 in the reaction of dot-immune analysis].
abstract: The source of monoclonal antibodies was chosen the cultural fluid of hybridoma-producers deposited in the specialized collection of cell cultures of vertebrates (St. Petersburg) with numbers RKKK(P) 386D and RKKK(P) 674D. The specific immunoglobulin (Ig) from cultural fluid was concentrated by precipitation with saturated solution of ammonium sulfate. The scheme of obtaining monoclonal antibodies included activation of peroxidase, conjugation of activated peroxidase with Ig, removal of unbounded proteins, storage and control. The preservation of activity of conjugates was supported with BSA (10%) or glycerin (50%). The last on is preferable to be applied for this purpose. The test of monoclonal antibody-01 and monoclonal antibody-0139 of peroxidase conjugates with kit of strains of comma bacillus 01 and 0139 demonstrated their strict specificity because they interacted only with corresponding serum groups under absence of crossed reactions with representatives of geterologic microorganisms. The direct dot-immune analysis is carried out during 1.5 hour and its sensitivity is within the limits 105-106. The application of diagnostic monoclonal peroxidase conjugates 01, 0139 in laboratory practice can promote the increase of specificity of serologic analysis of cholera and saving time-frame of its application.
authors.forename: L P
authors.initials: LP
authors.lastname: Alekseeva
authors.forename: G A
authors.initials: GA
authors.lastname: Kozlova
authors.forename: O V
authors.initials: OV
authors.lastname: Markina
authors.forename: O F
authors.initials: OF
authors.lastname: Kretenchuk
authors.forename: M E
authors.initials: ME
authors.lastname: Iagovkin
authors.forename: O S
authors.initials: OS
authors.lastname: Bursha
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000918:Antibody Specificity; D002771:Cholera / Q000097:blood / Q000175:diagnosis* / Q000382:microbiology; D002772:Cholera Toxin / Q000097:blood / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007136:Immunoglobulins / Q000097:blood*; D000922:Immunotoxins / Q000276:immunology; D009195:Peroxidase / Q000737:chemistry / Q000276:immunology*; D014734:Vibrio cholerae / Q000276:immunology / Q000302:isolation & purification
chemical_list: D000911:Antibodies, Monoclonal; D007136:Immunoglobulins; D000922:Immunotoxins; D002772:Cholera Toxin; D009195:Peroxidase