title: [Human umbilical cord mesenchymal stem cells reduce the sensitivity of HL-60 cells to cytarabine].
abstract: This study was purposed to investigate the impact of human umbilical cord-derived mesenchymal stem cells (hUC-MSC) on the sensitivity of HL-60 cells to therapeutic drugs so as to provide more information for exploring the regulatory effect of hUC-MSC on leukemia cells. Transwell and direct co-culture systems of HL-60 and hUC-MSC were established. The apoptosis and cell cycle of HL-60 cells were detected by flow cytometry. RT-PCR and Western blot were used to detect the mRNA and protein levels of Caspase 3, respectively. The results showed that the apoptosis of HL-60 induced by cytarabine (Ara-C) decreased significantly after direct co-cultured with hUC-MSC cycle mRNA (P < 0.05). The similar phenomenon was observed in transwell co-culture system. Cell cycle of HL-60 cells were arrested at G0/G1 phase and did not enter into S phase (P < 0.05) and the expression of Caspase-3 mRNA and protein in HL-60 cells were reduced (P < 0.05). It is concluded that hUC-MSC protected HL-60 from Arc-C induced apoptosis through regulating the cell cycle and down-regulating expression of Caspase 3 in HL-60 cells. In addition, this effect is caused by the soluble factors from hUC-MSC.
authors.affiliation: Chinese Academy of Medical Sciences, Tianjin, China.
authors.forename: Jun-Jie
authors.initials: JJ
authors.lastname: Cui
authors.forename: Ying
authors.initials: Y
authors.lastname: Chi
authors.forename: Wen-Jing
authors.initials: WJ
authors.lastname: Du
authors.forename: Shao-Guang
authors.initials: SG
authors.lastname: Yang
authors.forename: Xue
authors.initials: X
authors.lastname: Li
authors.forename: Fang
authors.initials: F
authors.lastname: Chen
authors.forename: Feng-Xia
authors.initials: FX
authors.lastname: Ma
authors.forename: Shi-Hong
authors.initials: SH
authors.lastname: Lu
authors.forename: Zhong-Chao
authors.initials: ZC
authors.lastname: Han
mesh_terms: D017209:Apoptosis; D053148:Caspase 3 / Q000378:metabolism; D018920:Coculture Techniques; D003561:Cytarabine / Q000494:pharmacology*; D018922:HL-60 Cells; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D014470:Umbilical Cord / Q000166:cytology*
chemical_list: D003561:Cytarabine; C505407:CASP3 protein, human; D053148:Caspase 3