title: A study of ovarian cancer biomarker amplification using ultrasound for early stage detection.
abstract: The application of serum biomarker to ovarian tumors for early stage detection and clinical diagnosis is a rapidly expanding research area. The problem with conventional markers is that they are often released too late or at too low a level to be detected in time to trigger effective treatment. Ultrasound has been used to influence bio-effects in living cells, but there is only one reported case of the use of ultrasound to enhance the release of a biomarker (Carcinoembryonic antigen CEA). In this study we report the use of ultrasound to enhance the release of a combination of ovarian cancer biomarkers (CA125 and CA19-9) to help in the diagnosis of ovarian cancer at an early stage. The results indicated that after 5 min sonication at a frequency of 1 MHz and intensity of 0.3 W cm(-2), the CA125 and CA19-9 levels were increased by 2.02 and 4.21-fold respectively. These findings suggest that ultrasonic treatment can be used to enhance the release of serum biomarkers from ovarian tumors.
authors.affiliation: Key Laboratory of Environment Medicine and Engineering, Ministry of Education, School of Public Health, Southeast University, Nanjing 210009, PR China; Department of Obstetrics and Gynecology, Zhongda Hospital, Southeast University, Nanjing 210009, PR China.
authors.forename: Danhong
authors.initials: D
authors.lastname: Peng
authors.forename: Tian
authors.initials: T
authors.lastname: Xu
authors.forename: Timothy J
authors.initials: TJ
authors.lastname: Mason
authors.forename: Wei
authors.initials: W
authors.lastname: Wu
mesh_terms: D014408:Biomarkers, Tumor / Q000378:metabolism*; D018394:CA-125 Antigen / Q000378:metabolism*; D018395:CA-19-9 Antigen / Q000378:metabolism*; D045744:Cell Line, Tumor; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000528:radiation effects*; D019220:High-Energy Shock Waves; D006801:Humans; D008565:Membrane Proteins / Q000378:metabolism*; D009367:Neoplasm Staging; D010051:Ovarian Neoplasms / Q000378:metabolism* / Q000473:pathology*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013010:Sonication / Q000379:methods*
chemical_list: D014408:Biomarkers, Tumor; D018394:CA-125 Antigen; D018395:CA-19-9 Antigen; C451759:MUC16 protein, human; D008565:Membrane Proteins
keywords: Amplification; Biomarker; Ovarian cancer cell line; Ultrasound