title: In vitro circadian period is associated with circadian/sleep preference.
abstract: Evaluation of circadian phenotypes is crucial for understanding the pathophysiology of diseases associated with disturbed biological rhythms such as circadian rhythm sleep disorders (CRSDs). We measured clock gene expression in fibroblasts from individual subjects and observed circadian rhythms in the cells (in vitro rhythms). Period length of the in vitro rhythm (in vitro period) was compared with the intrinsic circadian period, τ, measured under a forced desynchrony protocol (in vivo period) and circadian/sleep parameters evaluated by questionnaires, sleep log, and actigraphy. Although no significant correlation was observed between the in vitro and in vivo periods, the in vitro period was correlated with chronotype, habitual sleep time, and preferred sleep time. Our data demonstrate that the in vitro period is significantly correlated with circadian/sleep preference. The findings suggest that fibroblasts from individual patients can be utilized for in vitro screening of therapeutic agents to provide personalized therapeutic regimens for CRSD patients.
authors.affiliation: Department of Psychophysiology, National Institute of Mental Health, National Center of Neurology and Psychiatry, Kodaira, Tokyo 187-8553, Japan. hida@ncnp.go.jp
authors.forename: Akiko
authors.initials: A
authors.lastname: Hida
authors.forename: Shingo
authors.initials: S
authors.lastname: Kitamura
authors.forename: Yosuke
authors.initials: Y
authors.lastname: Ohsawa
authors.forename: Minori
authors.initials: M
authors.lastname: Enomoto
authors.forename: Yasuko
authors.initials: Y
authors.lastname: Katayose
authors.forename: Yuki
authors.initials: Y
authors.lastname: Motomura
authors.forename: Yoshiya
authors.initials: Y
authors.lastname: Moriguchi
authors.forename: Kentaro
authors.initials: K
authors.lastname: Nozaki
authors.forename: Makiko
authors.initials: M
authors.lastname: Watanabe
authors.forename: Sayaka
authors.initials: S
authors.lastname: Aritake
authors.forename: Shigekazu
authors.initials: S
authors.lastname: Higuchi
authors.forename: Mie
authors.initials: M
authors.lastname: Kato
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Kamei
authors.forename: Shin
authors.initials: S
authors.lastname: Yamazaki
authors.forename: Yu-Ichi
authors.initials: Y
authors.lastname: Goto
authors.forename: Masaaki
authors.initials: M
authors.lastname: Ikeda
authors.forename: Kazuo
authors.initials: K
authors.lastname: Mishima
mesh_terms: D000328:Adult; D002940:Circadian Rhythm; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D012890:Sleep; D011795:Surveys and Questionnaires; D055815:Young Adult