title: Inhibitory effects of osteoprotegerin on osteoclast formation and function under serum-free conditions.
abstract: The purpose of this study was to determine whether osteoprotegerin (OPG) could affect osteoclat differentiation and activation under serum-free conditions. Both duck embryo bone marrow cells and RAW264.7 cells were incubated with macrophage colony stimulatory factor (M-CSF) and receptor activator for nuclear factor kB ligand (RANKL) in serum-free medium to promote osteoclastogenesis. During cultivation, 0, 10, 20, 50, and 100 ng/mL OPG were added to various groups of cells. Osteoclast differentiation and activation were monitored via tartrate-resistant acid phosphatase (TRAP) staining, filamentous-actin rings analysis, and a bone resorption assay. Furthermore, the expression osteoclast-related genes, such as TRAP and receptor activator for nuclear factor κB (RANK), that was influenced by OPG in RAW264.7 cells was examined using real-time polymerase chain reaction. In summary, findings from the present study suggested that M-CSF with RANKL can promote osteoclast differentiation and activation, and enhance the expression of TRAP and RANK mRNA in osteoclasts. In contrast, OPG inhibited these activities under serum-free conditions.
authors.affiliation: College of Veterinary Medicine, Yangzhou University, and Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, China.
authors.forename: Ying-Xiao
authors.initials: YX
authors.lastname: Fu
authors.forename: Jian-Hong
authors.initials: JH
authors.lastname: Gu
authors.forename: Yi-Ran
authors.initials: YR
authors.lastname: Zhang
authors.forename: Xi-Shuai
authors.initials: XS
authors.lastname: Tong
authors.forename: Hong-Yan
authors.initials: HY
authors.lastname: Zhao
authors.forename: Yan
authors.initials: Y
authors.lastname: Yuan
authors.forename: Xue-Zhong
authors.initials: XZ
authors.lastname: Liu
authors.forename: Jian-Chun
authors.initials: JC
authors.lastname: Bian
authors.forename: Zong-Ping
authors.initials: ZP
authors.lastname: Liu
mesh_terms: D000135:Acid Phosphatase / Q000235:genetics / Q000378:metabolism; D000818:Animals; D030161:Avian Proteins / Q000494:pharmacology*; D001854:Bone Marrow Cells / Q000187:drug effects / Q000378:metabolism*; D002478:Cells, Cultured; D004372:Ducks; D004625:Embryo, Nonmammalian / Q000187:drug effects / Q000378:metabolism; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism; D016173:Macrophage Colony-Stimulating Factor / Q000378:metabolism; D010010:Osteoclasts / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D053244:Osteoprotegerin / Q000494:pharmacology*; D053245:RANK Ligand / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D053246:Receptor Activator of Nuclear Factor-kappa B / Q000235:genetics / Q000378:metabolism; D000071681:Tartrate-Resistant Acid Phosphatase
chemical_list: D030161:Avian Proteins; D007527:Isoenzymes; D053244:Osteoprotegerin; D053245:RANK Ligand; D053246:Receptor Activator of Nuclear Factor-kappa B; D016173:Macrophage Colony-Stimulating Factor; D000135:Acid Phosphatase; D000071681:Tartrate-Resistant Acid Phosphatase
keywords: activation; differentiation; osteoclast; osteoprotegerin; serum-free