title: Analysis of nanoscale protein film consisting of lactoferrin/11-MUA bilayers for bioelectronic device.
abstract: We fabricated and analyzed a nanoscale biofilm of human lactoferrin making use of 11-mercapto-undecanoic acid (11-MUA) as chemical linker. The fabrication of the bimolecular/organic hetero monolayer (lactoferrin/11-MUA) on gold substrate was confirmed with Raman spectroscopy. Cyclic voltammetry (CV) was carried out to observe the electrochemical properties of the nanoscaled biofilm under various pH conditions and at different time intervals. The well-defined redox properties were observed, even in certain harsh pH conditions and after a long time, proving the stabilities of this biofilm. Atomic force microscopy (AFM) was further employed to confirm the retention time by investigating the morphology variety of the biofilm over time. All these results proved that, the proposed nanoscaled thin film composed of lactoferrin and 11-MUA is a powerful alternative for making bioelectronics devices.
authors.affiliation: Department of Chemical and Biomolecular Engineering, Sogang University, 35 Baekbeom-ro, Mapo-gu, Seoul 121-742, Republic of Korea.
authors.forename: Qi
authors.initials: Q
authors.lastname: Chen
authors.forename: Taek
authors.initials: T
authors.lastname: Lee
authors.forename: Ajay Yagati
authors.initials: AY
authors.lastname: Kumar
authors.forename: Junhong
authors.initials: J
authors.lastname: Min
authors.forename: Jeong-Woo
authors.initials: JW
authors.lastname: Choi
mesh_terms: D057005:Bioengineering / Q000379:methods; D055615:Electrical Equipment and Supplies; D055664:Electrochemical Techniques; D005227:Fatty Acids / Q000737:chemistry*; D006801:Humans; D007781:Lactoferrin / Q000737:chemistry*; D008051:Lipid Bilayers / Q000032:analysis* / Q000737:chemistry; D018625:Microscopy, Atomic Force; D008954:Models, Biological; D046912:Multiprotein Complexes / Q000032:analysis* / Q000737:chemistry; D049329:Nanostructures / Q000737:chemistry; D010084:Oxidation-Reduction; D013059:Spectrum Analysis, Raman; D013438:Sulfhydryl Compounds / Q000737:chemistry*; D013499:Surface Properties
chemical_list: C505222:11-mercaptoundecanoic acid; D005227:Fatty Acids; D008051:Lipid Bilayers; D046912:Multiprotein Complexes; D013438:Sulfhydryl Compounds; D007781:Lactoferrin