title: Gastroprotective effect of an ethanolic extract from Neoglaziovia variegata (Arruda) Mez (Bromeliaceae) in rats and mice.
abstract: This study investigates the gastroprotective effect of a crude ethanolic extract of Neoglaziovia variegata (Arruda) Mez (Bromeliaceae), designated Nv-EtOH, in experimental models of gastric ulcer. In the ethanol-induced gastric ulcer model, Nv-EtOH showed gastroprotection at doses of 200 and 400 mg/kg body weight (BW) (57.0% and 79.7%, respectively). Nv-EtOH also significantly reduced the formation of gastric lesions induced by ethanol/HCl (31.6% and 63.5%), ibuprofen (70.0% and 74.3%), or ischemia/reperfusion in rats (65.0% and 87.0%) at 200 and 400 mg/kg BW when compared with the vehicle group. In the antioxidant activity assessment, Nv-EtOH (400 mg/kg BW) increased the catalase activity and sulfhydryl groups (SH) levels, respectively. Moreover, gastroprotection against ethanol damage was decreased after ibuprofen pretreatment. Nv-EtOH (400 mg/kg BW) promoted a significant increase in the content of gastric wall mucus. The Nv-EtOH effect was significantly reduced in mice pretreated with N(G)-nitro-L-arginine (L-NOARG) or glibenclamide, inhibitors of nitric oxide synthase and K(ATP) channel activation, respectively, suggesting the involvement of these mechanisms in the Nv-EtOH-induced gastroprotective effect. Nv-EtOH decreased the total acidity, but did not modify other gastric juice parameters. Nv-EtOH was also effective in promoting the healing process in chronic gastric ulcer induced by acetic acid in rats.
authors.affiliation: Medicinal Plants Research Center, Federal University of Piauí, Av. Nossa Senhora de Fátima s/n, 64049-550 Teresina, PI, Brazil.
authors.forename: Flávia Danniele F
authors.initials: FD
authors.lastname: Machado
authors.forename: Francilene V
authors.initials: FV
authors.lastname: Silva
authors.forename: Hélio B
authors.initials: HB
authors.lastname: Fernandes
authors.forename: Flávia Franceli B P
authors.initials: FF
authors.lastname: Freitas
authors.forename: Daniel D R
authors.initials: DD
authors.lastname: Arcanjo
authors.forename: Julianeli T
authors.initials: JT
authors.lastname: Lima
authors.forename: Jackson Roberto G S
authors.initials: JR
authors.lastname: Almeida
authors.forename: Francisco A
authors.initials: FA
authors.lastname: Oliveira
authors.forename: Rita C M
authors.initials: RC
authors.lastname: Oliveira
mesh_terms: D000818:Animals; D000897:Anti-Ulcer Agents / Q000494:pharmacology*; D031229:Bromeliaceae / Q000737:chemistry*; D002374:Catalase / Q000378:metabolism; D000431:Ethanol / Q000737:chemistry*; D005753:Gastric Mucosa / Q000378:metabolism; D008297:Male; D051379:Mice; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D011453:Prostaglandins / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013270:Stomach / Q000187:drug effects / Q000201:enzymology
chemical_list: D000897:Anti-Ulcer Agents; D010936:Plant Extracts; D011453:Prostaglandins; D000431:Ethanol; D002374:Catalase