title: [The new gene encoding TrfA-type replication initiator has been found on caprolactam/salicylate degradation plasmid pBS270].
abstract: The mini-replicon of pseudomonads' caprolactam/salicylate degradation plasmid pBS270 (105 kb, contains incompatibility determinants of P-7 group) has been obtained and its nucleotide sequence has been determined. The new gene encoding TrfA-like replication initiator has been found on this replicon. Poor homology of this replication initiator with known proteins of TrfA-family allows us to classify obtained replicon as IncP-1-like. The pBS270mini reveals chimeric nature.
authors.forename: O V
authors.initials: OV
authors.lastname: Volkova
authors.forename: A V
authors.initials: AV
authors.lastname: Panov
authors.forename: I A
authors.initials: IA
authors.lastname: Kosheleva
authors.forename: T Z
authors.initials: TZ
authors.lastname: Esikova
authors.forename: A M
authors.initials: AM
authors.lastname: Boronin
mesh_terms: D001483:Base Sequence; D001673:Biodegradation, Environmental; D002209:Caprolactam / Q000737:chemistry* / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000737:chemistry / Q000145:classification / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D010957:Plasmids / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D016958:Pseudomonas putida / Q000235:genetics* / Q000378:metabolism / Q000648:ultrastructure; D018741:Replication Origin / Q000235:genetics; D012093:Replicon / Q000235:genetics; D012459:Salicylates / Q000378:metabolism
chemical_list: D029968:Escherichia coli Proteins; D012459:Salicylates; C067405:TrfA protein, E coli; D002209:Caprolactam