title: Plant regeneration in Chlorophytum borivilianum Sant. et Fernand. from embryogenic callus and cell suspension culture and assessment of genetic fidelity of plants derived through somatic embryogenesis.
abstract: Efficient in vitro propagation of medicinally important endangered plant C. borivilianum has been achieved through somatic embryogenesis. Solid embryogenic medium [Murashige and Skoog medium containing 1.79 mM NH4NO3, 10.72 mM KNO3, 1.13 μM 2,4-dichlorophenoxyacetic acid, 7.38 μM 2-isopentenyladenine and 0.76 mM proline] supplemented with polyethylene glycol and sucrose (3 % each), exhibited 1.88-fold increase in embryo maturation compared to embryogenic medium containing 3 % sucrose. Liquid embryogenic medium supported better somatic embryo production and maturation. Highest total (79) and mature (cotyledonary stage) somatic embryos (38) as well as highest germination (57.5 %) was observed at inoculum density of 0.4 g/40 ml of liquid medium. 5.86 pH level exhibited optimal growth, maturation and germination of somatic embryos. Random amplified polymorphic DNA (RAPD) analysis of C. borivilianum plants regenerated through somatic embryogenesis revealed that they were genetically similar to the mother plant. The protocol established in the present study can be used for rapid mass multiplication of C. borivilianum in bioreactor employing liquid medium.
authors.affiliation: Plant Tissue Culture Division, Central Institute of Medicinal and Aromatic Plants (CIMAP), Lucknow, P.O. CIMAP-226015 Uttar Pradesh India ; Department of Botany, Shia Post Graduate College, Sitapur Road, Lucknow-226020, India.
authors.forename: Mohd Zahid
authors.initials: MZ
authors.lastname: Rizvi
authors.forename: Arun Kumar
authors.initials: AK
authors.lastname: Kukreja
authors.forename: Narendra Singh
authors.initials: NS
authors.lastname: Bisht
keywords: Chlorophytum borivilianum; Genetic fidelity; Maturation; Polyethylene glycol; Somatic embryos