title: Neuronal cells' behavior on polypyrrole coated bacterial nanocellulose three-dimensional (3D) scaffolds.
abstract: In this work, polypyrrole (PPy) was in situ polymerized onto the surface of bacterial nanocellulose (BNC) produced by Gluconacetobacter xylinus, by chemical oxidation in aqueous medium using ammonium persulfate. Composites (BNC/PPy) were produced with varying concentrations of pyrrole (Py). The produced BNC/PPy membranes were used as a template for the seeding of PC12 rat neuronal cells. Cell suspensions were directly seeded onto the surfaces of the BNC/PPy membranes. The Py concentration affected the behavior of neuronal cells that adhered and grew significantly more on BNC/PPy comparatively to BNC. Scanning electron microscopy (SEM) micrographs revealed that PC12 cells adhered on the surface of the BNC and BNC/PPy membranes. Conductive PPy coatings on nanofibers acting as an active interface for tissue engineering may be used to regulate cell activity through electrical stimulations.
authors.affiliation: Department of Biological Engineering, Centre of Biological Engineering, IBB-Institute for Biotechnology and Bioengineering, University of Minho, Campus de Gualtar, Braga, 4710-057, Portugal.
authors.forename: D
authors.initials: D
authors.lastname: Muller
authors.forename: J P
authors.initials: JP
authors.lastname: Silva
authors.forename: C R
authors.initials: CR
authors.lastname: Rambo
authors.forename: G M O
authors.initials: GM
authors.lastname: Barra
authors.forename: F
authors.initials: F
authors.lastname: Dourado
authors.forename: F M
authors.initials: FM
authors.lastname: Gama
mesh_terms: D000818:Animals; D002482:Cellulose / Q000737:chemistry*; D020099:Coated Materials, Biocompatible / Q000737:chemistry / Q000494:pharmacology*; D004553:Electric Conductivity; D008422:Materials Testing; D053758:Nanoparticles / Q000737:chemistry; D009474:Neurons / Q000187:drug effects* / Q000502:physiology; D016716:PC12 Cells; D011108:Polymers / Q000737:chemistry* / Q000494:pharmacology*; D011135:Polysaccharides, Bacterial / Q000737:chemistry; D011758:Pyrroles / Q000737:chemistry* / Q000494:pharmacology*; D051381:Rats; D013499:Surface Properties; D046509:Tissue Culture Techniques / Q000295:instrumentation; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D020099:Coated Materials, Biocompatible; D011108:Polymers; D011135:Polysaccharides, Bacterial; D011758:Pyrroles; C067635:polypyrrole; D002482:Cellulose