title: Genome-wide gene expression profiling, genotyping, and copy number analyses of acute myeloid leukemia using Affymetrix GeneChips.
abstract: With novel genome-wide technologies it is nowadays possible to perform detailed molecular analyses of normal and malignant tissues. Acute myeloid leukemia (AML) is a heterogeneous group of diseases with variable response to therapy. Gene expression profiling and genome-wide genotyping have recently been successfully applied to unravel the heterogeneity of AML. This chapter gives instructions and recommendations for genome-wide gene expression analyses, genotyping, and copy number analyses, as performed for AML using Affymetrix GeneChips.
authors.affiliation: Department of Hematology, Erasmus University Medical Center, Rotterdam, The Netherlands.
authors.forename: Mathijs A
authors.initials: MA
authors.lastname: Sanders
authors.forename: Peter J M
authors.initials: PJ
authors.lastname: Valk
mesh_terms: D056915:DNA Copy Number Variations / Q000235:genetics; D020869:Gene Expression Profiling; D015973:Gene Expression Regulation, Leukemic; D015894:Genome, Human; D060005:Genotyping Techniques / Q000379:methods*; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000235:genetics* / Q000473:pathology; D009154:Mutation; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*