title: Effect of Harderian adenectomy on the statistical analyses of mouse brain imaging using positron emission tomography.
abstract: Positron emission tomography (PET) using 2-deoxy-2-[(18)F] fluoro-D-glucose (FDG) as a radioactive tracer is a useful technique for in vivo brain imaging. However, the anatomical and physiological features of the Harderian gland limit the use of FDG-PET imaging in the mouse brain. The gland shows strong FDG uptake, which in turn results in distorted PET images of the frontal brain region. The purpose of this study was to determine if a simple surgical procedure to remove the Harderian gland prior to PET imaging of mouse brains could reduce or eliminate FDG uptake. Measurement of FDG uptake in unilaterally adenectomized mice showed that the radioactive signal emitted from the intact Harderian gland distorts frontal brain region images. Spatial parametric measurement analysis demonstrated that the presence of the Harderian gland could prevent accurate assessment of brain PET imaging. Bilateral Harderian adenectomy efficiently eliminated unwanted radioactive signal spillover into the frontal brain region beginning on postoperative Day 10. Harderian adenectomy did not cause any post-operative complications during the experimental period. These findings demonstrate the benefits of performing a Harderian adenectomy prior to PET imaging of mouse brains.
authors.affiliation: Department of Veterinary Medicine, College of Veterinary Medicine, Konkuk University, Seoul 143-701, Korea.
authors.forename: Minsoo
authors.initials: M
authors.lastname: Kim
authors.forename: Sang-Keun
authors.initials: SK
authors.lastname: Woo
authors.forename: Jung Woo
authors.initials: JW
authors.lastname: Yu
authors.forename: Yong Jin
authors.initials: YJ
authors.lastname: Lee
authors.forename: Kyeong Min
authors.initials: KM
authors.lastname: Kim
authors.forename: Joo Hyun
authors.initials: JH
authors.lastname: Kang
authors.forename: Kidong
authors.initials: K
authors.lastname: Eom
authors.forename: Sang-Soep
authors.initials: SS
authors.lastname: Nahm
mesh_terms: D000818:Animals; D001921:Brain / Q000000981:diagnostic imaging / Q000378:metabolism*; D019788:Fluorodeoxyglucose F18; D005625:Frontal Lobe / Q000000981:diagnostic imaging / Q000378:metabolism; D006243:Harderian Gland / Q000000981:diagnostic imaging / Q000378:metabolism / Q000601:surgery*; D051379:Mice; D008807:Mice, Inbred BALB C; D059906:Neuroimaging / Q000592:standards / Q000662:veterinary*; D049268:Positron-Emission Tomography / Q000662:veterinary*; D019275:Radiopharmaceuticals
chemical_list: D019275:Radiopharmaceuticals; D019788:Fluorodeoxyglucose F18
keywords: Harderian gland; adenectomy; mouse; positron emission tomography; statistical parametric mapping