title: Lipo-chitooligosaccharidic symbiotic signals are recognized by LysM receptor-like kinase LYR3 in the legume Medicago truncatula.
abstract: While chitooligosaccharides (COs) derived from fungal chitin are potent elicitors of defense reactions, structurally related signals produced by certain bacteria and fungi, called lipo-chitooligosaccharides (LCOs), play important roles in the establishment of symbioses with plants. Understanding how plants distinguish between friend and foe through the perception of these signals is a major challenge. We report the synthesis of a range of COs and LCOs, including photoactivatable probes, to characterize a membrane protein from the legume Medicago truncatula. By coupling photoaffinity labeling experiments with proteomics and transcriptomics, we identified the likely LCO-binding protein as LYR3, a lysin motif receptor-like kinase (LysM-RLK). LYR3, expressed heterologously, exhibits high-affinity binding to LCOs but not COs. Homology modeling, based on the Arabidopsis CO-binding LysM-RLK AtCERK1, suggests that LYR3 could accommodate the LCO in a conserved binding site. The identification of LYR3 opens up ways for the molecular characterization of LCO/CO discrimination.
authors.affiliation: INRA , Laboratoire des Interactions Plantes-Microorganismes (LIPM), UMR441, 31326 Castanet-Tolosan, France.
authors.forename: Judith
authors.initials: J
authors.lastname: Fliegmann
authors.forename: Sophie
authors.initials: S
authors.lastname: Canova
authors.forename: Christophe
authors.initials: C
authors.lastname: Lachaud
authors.forename: Sandra
authors.initials: S
authors.lastname: Uhlenbroich
authors.forename: Virginie
authors.initials: V
authors.lastname: Gasciolli
authors.forename: Carole
authors.initials: C
authors.lastname: Pichereaux
authors.forename: Michel
authors.initials: M
authors.lastname: Rossignol
authors.forename: Charles
authors.initials: C
authors.lastname: Rosenberg
authors.forename: Marie
authors.initials: M
authors.lastname: Cumener
authors.forename: Delphine
authors.initials: D
authors.lastname: Pitorre
authors.forename: Benoit
authors.initials: B
authors.lastname: Lefebvre
authors.forename: Clare
authors.initials: C
authors.lastname: Gough
authors.forename: Eric
authors.initials: E
authors.lastname: Samain
authors.forename: Sébastien
authors.initials: S
authors.lastname: Fort
authors.forename: Hugues
authors.initials: H
authors.lastname: Driguez
authors.forename: Boris
authors.initials: B
authors.lastname: Vauzeilles
authors.forename: Jean-Marie
authors.initials: JM
authors.lastname: Beau
authors.forename: Alessandra
authors.initials: A
authors.lastname: Nurisso
authors.forename: Anne
authors.initials: A
authors.lastname: Imberty
authors.forename: Julie
authors.initials: J
authors.lastname: Cullimore
authors.forename: Jean-Jacques
authors.initials: JJ
authors.lastname: Bono
mesh_terms: D000595:Amino Acid Sequence; D002686:Chitin / Q000031:analogs & derivatives*; D008055:Lipids / Q000737:chemistry; D046913:Medicago truncatula / Q000502:physiology*; D008565:Membrane Proteins / Q000737:chemistry / Q000378:metabolism; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry / Q000378:metabolism*; D010940:Plant Proteins / Q000737:chemistry / Q000378:metabolism*; D016415:Sequence Alignment; D013559:Symbiosis
chemical_list: D008055:Lipids; D008565:Membrane Proteins; D009844:Oligosaccharides; D010940:Plant Proteins; D002686:Chitin