title: Amelioration of superoxide dismutase on ventilator-induced lung injury by suppressing leukocyte in the lungs and systemic circulation.
abstract: Superoxide dismutase (SOD) is a free radical scavenger and a broad-spectrum antioxidant. Its anti-inflammatory and immunomodulatory effects have recently been noted. We studied the effects of this antioxidant on lung damage, oxidative stress, and inflammation in a model of ventilator-induced lung injury (VILI), using 8- to 12-wk-old Sprange-Dawley rats (n = 40). Animals were randomized and evenly divided into two experimental groups, low tidal volume (V(T)) ventilation (V(T) = 9 ml/kg) and high V(T) ventilation (V(T) = 28 ml/kg). Each group was evenly divided into two subgroups: ten animals were treated with superoxide dismutase (SOD; 10,000 U/kg i.v., 2 h prior to the ventilation) and the rests were treated with vehicle. Lung injury was evaluated by histological examination, and cells counts of red blood cells (RBC) and white blood cells (WBC) in the alveoli and the septal wall thickness in lung tissues and serum lactate dehydrogenase (LDH). The lung permeability was assessed by the wet-to-dry weight ratio (W/D), lung weight to body weight ratio (LW/BW) and protein concentration in broncholavage fluid (BALF). Levels of oxidative stress and lipid peroxidation in the lungs were evaluated by tissue malondialdehyde (MDA) and methylguanidine (MG) in BALF, respectively. SOD pretreatment significantly decreased WBC counts in systemic circulation and in alveoli, and effectively attenuated high V(T) ventilation induced lung injury by reducing hyaline membrane development, septal wall thickness, lung W/D and LW/BW and serum LDH in relation to those of the control. In addition, lung tissues MDA and MG in BALF were also notably reduced.
authors.affiliation: School of Respiratory Therapy, Taipei Medical University, Taipei 11031, Taiwan, Republic of China.
authors.forename: Chien-Ling
authors.initials: CL
authors.lastname: Su
authors.affiliation: School of Medicine, College of Medicine, Fu Jen Catholic University, New Taipei City 24205, Taiwan, Republic of China.
authors.forename: Wen-Yuan
authors.initials: WY
authors.lastname: Du
authors.affiliation: School of Respiratory Therapy, Taipei Medical University, Taipei 11031, Taiwan, Republic of China.
authors.forename: Ling-Ling
authors.initials: LL
authors.lastname: Chiang
authors.affiliation: Biostatistics and Research Consultation Center, Taipei Medical University, Taipei 11031,Taiwan, Republic of China.
authors.forename: Yen-Kuang
authors.initials: YK
authors.lastname: Lin
authors.affiliation: Division of Pulmonary Medicine, Taipei Medical University-Shuang Ho Hospital, New Taipei City 23561, Taiwan, Republic of China.
authors.forename: Hui-Ling
authors.initials: HL
authors.lastname: Lee
authors.affiliation: Jen-The Junior College of Medicine, Nursing and Management, Maoli County 35664, Taiwan, Republic of China.
authors.forename: Kuan-Hao
authors.initials: KH
authors.lastname: Chen
authors.affiliation: School of Medicine, College of Medicine, Fu Jen Catholic University, New Taipei City 24205, Taiwan, Republic of China.
authors.forename: Jiun-
authors.initials: J
authors.lastname: Wang
authors.affiliation: School of Medicine, College of Medicine, Fu Jen Catholic University, New Taipei City 24205, Taiwan, Republic of China.
authors.forename: David
authors.initials: D
authors.lastname: Wang
mesh_terms: D000818:Animals; D007958:Leukocyte Count; D007962:Leukocytes / Q000187:drug effects*; D008168:Lung / Q000473:pathology*; D008297:Male; D018384:Oxidative Stress; D010100:Oxygen / Q000097:blood; D051381:Rats; D017207:Rats, Sprague-Dawley; D013482:Superoxide Dismutase / Q000627:therapeutic use*; D055397:Ventilator-Induced Lung Injury / Q000188:drug therapy* / Q000473:pathology / Q000503:physiopathology
chemical_list: D013482:Superoxide Dismutase; D010100:Oxygen