title: Immobilization of glutaminase enzyme from Hypocria jecorina on polyacrylic acid: preparation and biochemical characterization.
abstract: L-glutaminase enzyme produced from Hypocrea jecorina pure culture and polyacrylic acid (PAA) in the presence (Cu2+) ions were composed the ternary complex at pH 7. The properties of free and immobilized enzyme were defined. The effect of various factors such as pH, temperature, heat, and storage stability on immobilized enzyme were investigated. The properties of immobilized enzyme were investigated and compared to those of free enzyme. Optimum pH and temperature of both enzyme were determined to be 8.0 and 50°C, respectively. Kinetic parameters of the immobilized enzyme (Km and Vmax values) were also determined as 0.38 mM of the Km and 10.9 U/L of the Vmax. No drastic change was observed in the Km and Vmax values. Thermal and storage stability experiments were carried out. The thermal stability studies indicated that the immobilization process tends to stabilize the enzyme.
authors.affiliation: Department of Bioengineering, Faculty of Engineering and Natural Sciences, Uskudar University , Uskudar-Istanbul , Turkey.
authors.forename: Mesut
authors.initials: M
authors.lastname: Karahan
authors.forename: Emine
authors.initials: E
authors.lastname: Karakuş
authors.forename: Dilara
authors.initials: D
authors.lastname: Bülbül
authors.forename: Neşe
authors.initials: N
authors.lastname: Atacı
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry*; D003300:Copper / Q000737:chemistry; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry* / Q000378:metabolism*; D005972:Glutaminase / Q000737:chemistry* / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D035901:Hypocrea / Q000201:enzymology*; D007700:Kinetics; D013696:Temperature; D014867:Water / Q000737:chemistry
chemical_list: D000180:Acrylic Resins; D004800:Enzymes, Immobilized; D014867:Water; C006903:carbopol 940; D003300:Copper; D005972:Glutaminase
keywords: L-Glutaminase enzyme; enzyme production; immobilization; microorganism; polyacrylic acid; ternary metal complexes