title: Effect of mild hypothermia on the coagulation-fibrinolysis system and physiological anticoagulants after cardiopulmonary resuscitation in a porcine model.
abstract: The aim of this study was to evaluate the effect of mild hypothermia on the coagulation-fibrinolysis system and physiological anticoagulants after cardiopulmonary resuscitation (CPR). A total of 20 male Wuzhishan miniature pigs underwent 8 min of untreated ventricular fibrillation and CPR. Of these, 16 were successfully resuscitated and were randomized into the mild hypothermia group (MH, n = 8) or the control normothermia group (CN, n = 8). Mild hypothermia (33°C) was induced intravascularly, and this temperature was maintained for 12 h before pigs were actively rewarmed. The CN group received normothermic post-cardiac arrest (CA) care for 72 h. Four animals were in the sham operation group (SO). Blood samples were taken at baseline, and 0.5, 6, 12, 24, and 72 h after ROSC. Whole-body mild hypothermia impaired blood coagulation during cooling, but attenuated blood coagulation impairment at 72 h after ROSC. Mild hypothermia also increased serum levels of physiological anticoagulants, such as PRO C and AT-III during cooling and after rewarming, decreased EPCR and TFPI levels during cooling but not after rewarming, and inhibited fibrinolysis and platelet activation during cooling and after rewarming. Finally, mild hypothermia did not affect coagulation-fibrinolysis, physiological anticoagulants, or platelet activation during rewarming. Thus, our findings indicate that mild hypothermia exerted an anticoagulant effect during cooling, which may have inhibitory effects on microthrombus formation. Furthermore, mild hypothermia inhibited fibrinolysis and platelet activation during cooling and attenuated blood coagulation impairment after rewarming. Slow rewarming had no obvious adverse effects on blood coagulation.
authors.affiliation: Department of Emergency, First Hospital affiliated to Dalian Medical University, Dalian, Liaoning, PR China.
authors.forename: Ping
authors.initials: P
authors.lastname: Gong
authors.forename: Ming-Yue
authors.initials: MY
authors.lastname: Zhang
authors.forename: Hong
authors.initials: H
authors.lastname: Zhao
authors.forename: Zi-Ren
authors.initials: ZR
authors.lastname: Tang
authors.forename: Rong
authors.initials: R
authors.lastname: Hua
authors.forename: Xue
authors.initials: X
authors.lastname: Mei
authors.forename: Juan
authors.initials: J
authors.lastname: Cui
authors.forename: Chun-Sheng
authors.initials: CS
authors.lastname: Li
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D000925:Anticoagulants / Q000097:blood*; D000990:Antithrombin III / Q000378:metabolism; D001777:Blood Coagulation / Q000502:physiology*; D016887:Cardiopulmonary Resuscitation; D004195:Disease Models, Animal; D005342:Fibrinolysis / Q000502:physiology*; D006323:Heart Arrest / Q000503:physiopathology / Q000628:therapy; D006801:Humans; D007036:Hypothermia, Induced / Q000379:methods*; D008074:Lipoproteins / Q000097:blood; D008297:Male; D011486:Protein C / Q000378:metabolism; D011897:Random Allocation; D017694:Rewarming / Q000379:methods; D013552:Swine; D013556:Swine, Miniature; D013997:Time Factors; D014693:Ventricular Fibrillation / Q000503:physiopathology / Q000628:therapy
chemical_list: D000925:Anticoagulants; D008074:Lipoproteins; D011486:Protein C; C051928:lipoprotein-associated coagulation inhibitor; D000990:Antithrombin III