title: Cross-sectional study of hepatitis B virus infection in rural communities, Central African Republic.
abstract: BACKGROUND
  As most data on hepatitis in resource-poor countries relate to urban communities, surveys in the rural environment are necessary to determine the 'true' prevalence of these viral infections. We undertook a survey to determine the prevalence of hepatitis B virus (HBV) infection in an apparently healthy rural population in the Central African Republic (CAR).
  
  
  METHODS
  The cross-sectional study was based on dried blood spots (DBS) from 273 people recruited in four prefectures (Lobaye, Nana-Mambéré, Ouham and Ouaka). Eluates from DBS were tested with commercial ELISA kits to detect markers of HBV infection. DBS were directly used for DNA extraction, followed by PCR and genotyping based on preS/S gene sequences.
  
  
  RESULTS
  The overall prevalence of HBc antibodies was 27.1% (Lobaye 29%, Nana-Mambéré 28%, Ouaka 29% and Ouham 23%) and that of HBsAg was 10.6% (Lobaye 9%, Nana-Mambéré 9%, Ouaka 19% and Ouham 8%), with no statistically significant difference among the surveyed communities. Nineteen sequences obtained from 74 anti-HBc-positive patients all belonged to genotype E. Risk factor analysis of HBV infection pointed to sexual transmission of the virus.
  
  
  CONCLUSIONS
  The prevalence of HBV is high in rural communities in the CAR and comparable to that observed in urban areas. In addition, genotype E is prevalent in these areas. These findings underline the importance of instituting a programme of active HBV surveillance and vaccination of the population.
authors.affiliation: Viral Hepatitis Laboratory, Institut Pasteur de Bangui, PO Box 923, Bangui, Central African Republic. npkomas@yahoo.fr
authors.forename: Narcisse P
authors.initials: NP
authors.lastname: Komas
authors.forename: Ulrich
authors.initials: U
authors.lastname: Vickos
authors.forename: Judith M
authors.initials: JM
authors.lastname: Hübschen
authors.forename: Aubin
authors.initials: A
authors.lastname: Béré
authors.forename: Alexandre
authors.initials: A
authors.lastname: Manirakiza
authors.forename: Claude P
authors.initials: CP
authors.lastname: Muller
authors.forename: Alain
authors.initials: A
authors.lastname: Le Faou
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D002488:Central African Republic / Q000453:epidemiology; D002648:Child; D002675:Child, Preschool; D003430:Cross-Sectional Studies; D005260:Female; D060005:Genotyping Techniques; D006509:Hepatitis B / Q000453:epidemiology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D016017:Odds Ratio; D010802:Phylogeny; D015995:Prevalence; D012307:Risk Factors; D012424:Rural Population / Q000706:statistics & numerical data; D055815:Young Adult