title: Measurement of cell respiration and oxygenation in standard multichannel biochips using phosphorescent O2-sensitive probes.
abstract: Measurement of cell oxygenation and oxygen consumption is useful for studies of cell bioenergetics, metabolism, mitochondrial function, drug toxicity and common pathophysiological conditions. Here we present a new platform for such applications which uses commercial multichannel biochips (μ-slides, Ibidi) and phosphorescent O2 sensitive probes. This platform was evaluated with both extracellular and intracellular O2 probes, several different cell types and treatments including mitochondrial uncoupling and inhibition, depletion of extracellular Ca(2+) and inhibition of V-ATPase and histone deacetylases. The results show that compared to the standard microwell plates currently used, the μ-slide platform provides facile O2 measurements with both suspension and adherent cells, higher sensitivity and reproducibility, and faster measurement time. It also allows re-perfusion and multiple treatments of cells and multi-parametric analyses in conjunction with other probes. Optical measurements are conducted on standard fluorescence readers and microscopes.
authors.affiliation: Biochemistry Department, University College Cork, Cork, Ireland.
authors.forename: Alina V
authors.initials: AV
authors.lastname: Kondrashina
authors.forename: Dmitri B
authors.initials: DB
authors.lastname: Papkovsky
authors.forename: Ruslan I
authors.initials: RI
authors.lastname: Dmitriev
mesh_terms: D000818:Animals; D019069:Cell Respiration; D006801:Humans; D049408:Luminescent Agents / Q000378:metabolism*; D051379:Mice; D046210:Microfluidic Analytical Techniques / Q000379:methods* / Q000592:standards; D010100:Oxygen / Q000378:metabolism*; D051381:Rats; D012015:Reference Standards
chemical_list: D049408:Luminescent Agents; D010100:Oxygen