title: Denaturing high-performance liquid chromatography for mutation detection and genotyping.
abstract: Denaturing high-performance liquid chromatography (DHPLC) is an accurate and efficient screening technique used for detecting DNA sequence changes by heteroduplex analysis. It can also be used for genotyping of single nucleotide polymorphisms (SNPs). The high sensitivity of DHPLC has made this technique one of the most reliable approaches to mutation analysis and, therefore, used in various areas of genetics, both in the research and clinical arena. This chapter describes the methods used for mutation detection analysis and the genotyping of SNPs by DHPLC on the WAVE™ system from Transgenomic Inc. ("WAVE" and "DNASep" are registered trademarks, and "Navigator" is a trademark, of Transgenomic, used with permission. All other trademarks are property of the respective owners).
authors.affiliation: Department of Human Genetics, University of Chicago, Chicago, IL, USA.
authors.forename: Donna Lee
authors.initials: DL
authors.lastname: Fackenthal
authors.forename: Pei Xian
authors.initials: PX
authors.lastname: Chen
authors.forename: Ted
authors.initials: T
authors.lastname: Howe
authors.forename: Soma
authors.initials: S
authors.lastname: Das
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004252:DNA Mutational Analysis / Q000379:methods*; D060005:Genotyping Techniques / Q000379:methods*; D020180:Heteroduplex Analysis / Q000379:methods*; D006801:Humans; D009154:Mutation; D009691:Nucleic Acid Denaturation; D020641:Polymorphism, Single Nucleotide