title: The fission yeast β-arrestin-like protein Any1 is involved in TSC-Rheb signaling and the regulation of amino acid transporters.
abstract: Rheb GTPase and the Tsc1-Tsc2 protein complex, which serves as a GTPase-activating protein for Rheb, have crucial roles in the regulation of cell growth in response to extracellular conditions. In Schizosaccharomyces pombe, Rheb and Tsc1-Tsc2 regulate cell cycle progression, the onset of meiosis and the uptake of amino acids. In cells lacking Tsc2 (Δtsc2), the amino acid transporter Aat1, which is normally expressed on the plasma membrane under starvation conditions, is confined to the Golgi. Here, we show that the loss of either pub1(+), encoding an E3 ubiquitin ligase, or any1(+), encoding a β-arrestin-like protein, allows constitutive expression of Aat1 on the plasma membrane in Δtsc2 cells, suggesting that Pub1 and Any1 are required for localization of Aat1 to the Golgi. Subsequent analysis revealed that, in the Golgi, Pub1 and Any1 form a complex that ubiquitylates Aat1. Physical interaction of Pub1 and Any1 is more stable in Δtsc2 cells than in wild-type cells and is independent of Tor2 activity. These results indicate that the TSC-Rheb signaling pathway regulates the localization of amino acid transporters via Pub1 and Any1 in a Tor2-independent manner. Our study demonstrates that, unlike in budding yeast (in which Rsp5 and ARTs, a pair of proteins analogous to Pub1 and Any1, respectively, primarily act to reduce expression of the transporters on plasma membrane when nutrients are abundant), the primary role of fission yeast Pub1 and Any1 is to store the transporter in the Golgi under nutrient-rich conditions.
authors.affiliation: Radiation Biology Center, Kyoto University, Yoshida-Konoe cho, Sakyo ku, Kyoto 606-8501, Japan. nakase@house.rbc.kyoto-u.ac.jp
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Nakase
authors.forename: Mai
authors.initials: M
authors.lastname: Nakase
authors.forename: Jun
authors.initials: J
authors.lastname: Kashiwazaki
authors.forename: Tomoka
authors.initials: T
authors.lastname: Murai
authors.forename: Yoko
authors.initials: Y
authors.lastname: Otsubo
authors.forename: Issei
authors.initials: I
authors.lastname: Mabuchi
authors.forename: Masayuki
authors.initials: M
authors.lastname: Yamamoto
authors.forename: Kaoru
authors.initials: K
authors.lastname: Takegawa
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Matsumoto
mesh_terms: D026923:Amino Acid Transport Systems, Basic / Q000096:biosynthesis / Q000378:metabolism; D019390:Arrestins / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D019731:Carbon-Nitrogen Ligases / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D002453:Cell Cycle; D002462:Cell Membrane / Q000378:metabolism; D006056:Golgi Apparatus / Q000378:metabolism; D008540:Meiosis; D020559:Monomeric GTP-Binding Proteins / Q000378:metabolism*; D046912:Multiprotein Complexes / Q000378:metabolism; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism; D012568:Schizosaccharomyces; D029702:Schizosaccharomyces pombe Proteins / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction; D000071557:beta-Arrestins
chemical_list: D026923:Amino Acid Transport Systems, Basic; D019390:Arrestins; D019731:Carbon-Nitrogen Ligases; D020559:Monomeric GTP-Binding Proteins; D046912:Multiprotein Complexes; D019869:Phosphatidylinositol 3-Kinases; D029702:Schizosaccharomyces pombe Proteins; D000071557:beta-Arrestins; C588004:Any1 protein, S pombe; C471572:Tsc1 protein, S pombe; C471573:Tsc2 protein, S pombe; C513100:tor2 protein, S pombe; C530693:Rheb protein, S pombe; C098922:Pub1 protein, S pombe
keywords: Amino acid transporter; Any1; Arrestin; Schizosaccharomyces pombe; TSC; Tuberous sclerosis complex; Ubiquitin ligase