title: Hepatotoxicity evaluation of aqueous extract from Scutia buxifolia.
abstract: Nowadays there is an increase in the number of people taking herbals worldwide. Scutia buxifolia is used for the treatment of hypertension, but little is known about its action on liver. Thirty-two Wistar rats were divided into four groups: control and groups treated during 30 days with 100, 200 and 400 mg of lyophilized aqueous extract of S. buxifolia (SBSB)/kg of body weight. This study was planned to explore hepatotoxic effect of SBSB, which was assessed by serum transaminases (ALT and AST). Thiobarbituric acid reactive substances (TBARS) levels were determined in liver, along with thiols content (NPSH), catalase (CAT) activity and, superoxide dismutase (SOD) enzymes. Histopathological studies of liver tissue were performed. Flavonoids and phenolics were quantified in SBSB by high performance liquid chromatography with diode array detection (HPLC/DAD). We did not observe alterations on redox status (TBARS, NPSH, CAT and, SOD) in the control and experimental groups. An increase on AST activity was only observed at 200 mg of SBSB, whereas ALT score was not affected by SBSB. Moreover, no morphological alterations were observed on the hepatocytes, matching the analysed biochemical parameters. This way, we conclude that SBSB was not toxic.
authors.affiliation: Graduate Program in Pharmaceutical Sciences, Center of Health Sciences, Federal University of Santa Maria, Santa Maria, RS 97105-900, Brazil.
authors.forename: Robson Borba
authors.initials: RB
authors.lastname: de Freitas
authors.forename: Bruno Tomazele
authors.initials: BT
authors.lastname: Rovani
authors.forename: Aline Augusti
authors.initials: AA
authors.lastname: Boligon
authors.forename: Thiele Faccim
authors.initials: TF
authors.lastname: de Brum
authors.forename: Mariana
authors.initials: M
authors.lastname: Piana
authors.forename: Roberta
authors.initials: R
authors.lastname: da Silva Jesus
authors.forename: Carolina Fantinel
authors.initials: CF
authors.lastname: Veloso
authors.forename: Helena
authors.initials: H
authors.lastname: Kober
authors.forename: Rafael Noal
authors.initials: RN
authors.lastname: Moresco
authors.forename: Isabel Cristina
authors.initials: IC
authors.lastname: da Costa Araldi
authors.forename: Liliane
authors.initials: L
authors.lastname: de Freitas Bauermann
authors.forename: Margareth Linde
authors.initials: ML
authors.lastname: Athayde
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000032:analysis; D002374:Catalase / Q000187:drug effects / Q000378:metabolism; D005419:Flavonoids / Q000032:analysis; D029001:Herbal Medicine; D008099:Liver / Q000187:drug effects*; D008111:Liver Function Tests; D010084:Oxidation-Reduction / Q000187:drug effects; D010936:Plant Extracts / Q000633:toxicity*; D051381:Rats; D017208:Rats, Wistar; D027823:Rhamnaceae / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000187:drug effects / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis; D000637:Transaminases / Q000187:drug effects
chemical_list: D000975:Antioxidants; D005419:Flavonoids; D010936:Plant Extracts; D017392:Thiobarbituric Acid Reactive Substances; D002374:Catalase; D013482:Superoxide Dismutase; D000637:Transaminases