title: MicroRNA in Alzheimer's disease: an exploratory study in brain, cerebrospinal fluid and plasma.
abstract: MicroRNA (miRNA) may be potential biomarkers of Alzheimer's disease (AD). The objective of this investigation was to demonstrate that miRNAs in human brain or biofluids are differentially expressed according to disease status, tissue type, neuritic plaque score or Braak stage. Post-mortem brain (PMB) miRNA were profiled using arrays and validated using quantitative RT-PCR (qRT-PCR). Five qRT-PCR-validated miRNAs were measured in an independent sample of PMB, cerebrospinal fluid and plasma from the same subjects. Plasma miR-15a was found to be associated with plaque score in the independent sample. In conclusion, miRNA present in human biofluids may offer utility as biomarkers of AD.
authors.affiliation: Department of Geriatric (GRECC), Research, Education, and Clinical Centers, VA Puget Sound Health Care System, Seattle, WA 98108, USA. lbekris@uw.edu
authors.forename: Lynn M
authors.initials: LM
authors.lastname: Bekris
authors.forename: Franziska
authors.initials: F
authors.lastname: Lutz
authors.forename: Thomas J
authors.initials: TJ
authors.lastname: Montine
authors.forename: Chang En
authors.initials: CE
authors.lastname: Yu
authors.forename: Debby
authors.initials: D
authors.lastname: Tsuang
authors.forename: Elaine R
authors.initials: ER
authors.lastname: Peskind
authors.forename: James B
authors.initials: JB
authors.lastname: Leverenz
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D000544:Alzheimer Disease / Q000097:blood / Q000134:cerebrospinal fluid* / Q000175:diagnosis; D019540:Area Under Curve; D015415:Biomarkers / Q000097:blood / Q000134:cerebrospinal fluid; D001921:Brain / Q000378:metabolism*; D016022:Case-Control Studies; D045744:Cell Line, Tumor; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000097:blood / Q000134:cerebrospinal fluid* / Q000235:genetics; D008875:Middle Aged; D034622:RNA Interference; D012372:ROC Curve; D059467:Transcriptome
chemical_list: D015415:Biomarkers; D035683:MicroRNAs