title: Imaging cell biology in live animals: ready for prime time.
abstract: Time-lapse fluorescence microscopy is one of the main tools used to image subcellular structures in living cells. Yet for decades it has been applied primarily to in vitro model systems. Thanks to the most recent advancements in intravital microscopy, this approach has finally been extended to live rodents. This represents a major breakthrough that will provide unprecedented new opportunities to study mammalian cell biology in vivo and has already provided new insight in the fields of neurobiology, immunology, and cancer biology.
authors.affiliation: Oral and Pharyngeal Cancer Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD 20892, USA. weigertr@mail.nih.gov
authors.forename: Roberto
authors.initials: R
authors.lastname: Weigert
authors.forename: Natalie
authors.initials: N
authors.lastname: Porat-Shliom
authors.forename: Panomwat
authors.initials: P
authors.lastname: Amornphimoltham
mesh_terms: D000818:Animals; D003585:Cell Biology / Q000639:trends; D002478:Cells, Cultured; D003584:Cytological Techniques; D049452:Green Fluorescent Proteins / Q000032:analysis; D006801:Humans; D051379:Mice; D008856:Microscopy, Fluorescence / Q000295:instrumentation / Q000379:methods*; D059008:Time-Lapse Imaging / Q000295:instrumentation / Q000379:methods*
chemical_list: D049452:Green Fluorescent Proteins