title: Next generation sequencing yields the complete mitochondrial genome of the scarce blue-tailed damselfly, Ischnura pumilio.
abstract: We report the entire mitochondrial genome of the scarce blue-tailed damselfly, Ischnura pumilio (Odonata, Coenagrionidae), using next-generation sequencing on genomic DNA. A de novo assembly provided a single contiguous sequence of 15,250 bp that contained the A + T-rich region and all standard coding regions; gene configuration is similar to other odonates and comprises 13 protein-coding genes, two rRNA genes (12 S and 16 S rRNA) and 22 tRNA genes. We found a unique intergenic spacer in I. pumilio and confirm that the intergenic spacer s5 likely represents a synapomorphy between Anisoptera and Zygoptera. This is the first mitogenome sequence obtained for a member of the Coenagrionidae and demonstrates how next-generation sequencing technology can obtain mtDNA genome sequences without prior sample processing or primer design.
authors.affiliation: Department of Evolution, Ecology and Behaviour, Institute of Integrative Biology, University of Liverpool , Liverpool , UK , and.
authors.forename: M Olalla
authors.initials: MO
authors.lastname: Lorenzo-Carballa
authors.forename: David J
authors.initials: DJ
authors.lastname: Thompson
authors.forename: Adolfo
authors.initials: A
authors.lastname: Cordero-Rivera
authors.forename: Phillip C
authors.initials: PC
authors.lastname: Watts
mesh_terms: D000818:Animals; D054629:Genome, Mitochondrial; D019476:Insect Proteins / Q000235:genetics; D063191:Odonata / Q000235:genetics*; D012335:RNA, Ribosomal / Q000235:genetics; D012343:RNA, Transfer / Q000235:genetics
chemical_list: D019476:Insect Proteins; D012335:RNA, Ribosomal; D012343:RNA, Transfer
keywords: Damselfly; Odonata; intergenic spacer; mitochondrial genome