title: SbacHTS: spatial background noise correction for high-throughput RNAi screening.
abstract: BACKGROUND
  High-throughput cell-based phenotypic screening has become an increasingly important technology for discovering new drug targets and assigning gene functions. Such experiments use hundreds of 96-well or 384-well plates, to cover whole-genome RNAi collections and/or chemical compound files, and often collect measurements that are sensitive to spatial background noise whose patterns can vary across individual plates. Correcting these position effects can substantially improve measurement accuracy and screening success.
  
  
  RESULTS
  We developed SbacHTS (Spatial background noise correction for High-Throughput RNAi Screening) software for visualization, estimation and correction of spatial background noise in high-throughput RNAi screens. SbacHTS is supported on the Galaxy open-source framework with a user-friendly open access web interface. We find that SbacHTS software can effectively detect and correct spatial background noise, increase signal to noise ratio and enhance statistical detection power in high-throughput RNAi screening experiments.
  
  
  BACKGROUND
  http://www.galaxy.qbrc.org/
authors.affiliation: Quantitative Biomedical Research Center, Department of Clinical Science, Harold C. Simmons Comprehensive Cancer Center and Department of Cell Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA.
authors.forename: Rui
authors.initials: R
authors.lastname: Zhong
authors.forename: Min Soo
authors.initials: MS
authors.lastname: Kim
authors.forename: Michael A
authors.initials: MA
authors.lastname: White
authors.forename: Yang
authors.initials: Y
authors.lastname: Xie
authors.forename: Guanghua
authors.initials: G
authors.lastname: Xiao
mesh_terms: D023281:Genomics / Q000379:methods; D057166:High-Throughput Screening Assays / Q000379:methods*; D034622:RNA Interference; D012984:Software