title: [Simultaneous determination of 11 polyphenols in ratafee by reversed-phase high performance liquid chromatography].
abstract: OBJECTIVE
  To develop a novel quantitative method for simultaneous analysis of 11 polyphenols in ratafee by reversed-phase high performance liquid chromatography.
  
  
  METHODS
  The separation was performed on a Agilent Eclipse XDB C18 (4.6 mmx250 mm, 51 m) and the column temperature was set at 30 degrees C. The mobile phase was consisted of 0. 1% phosphoric acid (A) and methanol (B), with a linear gradient elution at a flow rate of I. 0 ml/min. The gradient program was as follow: 10% B isocratic (0-10 min), 10%-20% B liner (10-15 min), 20% B isocratic (15-25 min), 20%-60% B liner (25-65 min), 60% B isocratic (65-75 min). And detection wavelength was 210, 270, 320, 327 and 360 nm.
  
  
  RESULTS
  The correlation coefficients of the 11 calibration curves were all higher than 0.9995. The scopes of the recovery rate was within the range of 85.1% to 112.0%, with RSDs no more than 2%. The limit of quantification (LOQ) and the detection limit (LOD) were 0.32-1.59 ng and 0.10-0.48 ng, respectively.
  
  
  CONCLUSIONS
  The method is accurate and reliable, and can successfully analyze different ratafee samples from different sources.
authors.affiliation: National Institute of Nutrition and Food Safety, Chinese Center for Disease Control and Prevention, Beijing, China. hbxuweisheng@163.com
authors.forename: Weisheng
authors.initials: W
authors.lastname: Xu
authors.forename: Dong
authors.initials: D
authors.lastname: Li
authors.forename: Jing
authors.initials: J
authors.lastname: Liu
authors.forename: Yuexin
authors.initials: Y
authors.lastname: Yang
mesh_terms: D002392:Catechin / Q000032:analysis; D002726:Chlorogenic Acid / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D056148:Chromatography, Reverse-Phase / Q000379:methods*; D005707:Gallic Acid / Q000032:analysis; D059808:Polyphenols / Q000032:analysis*; D012680:Sensitivity and Specificity; D014920:Wine / Q000032:analysis*
chemical_list: D059808:Polyphenols; D002726:Chlorogenic Acid; D005707:Gallic Acid; D002392:Catechin