title: Enzymatic degradation and bioactivity evaluation of C-6 oxidized chitosan.
abstract: C-6 oxidized chitosan was produced from chitosan by performing selective oxidation with NaOCl and NaBr using 2,2,6,6-tetramethylpiperidine-1-oxy radical (TEMPO) as catalyst. Endocellulase, Celluclast 1.5 L, Glucanex(®), Macerozyme R-10, hyaluronidase, hyaluronate lyase, red scorpionfish chitinase, glucuronan lyase and a protein mix from Trichoderma reesei were used to degrade the C-6 oxidized chitosan. Glucanex(®), the crude extract from T. reesei IHEM 4122 and Macerozyme R-10 validated the enzymatic degradation through final hydrolysis yields of the derivative respectively close to 36.4, 20.3 and 12.9% (w/w). The best initial reaction velocity (2.41 U/mL) was observed for Glucanex(®). The antileishmanial activity of the derivative was evaluated against Leishmania infantum LIPA 137. The antibacterial activities against Escherichia coli ATCC 25922 and Pseudomonas aeruginosa ATCC 27853 were also tested. Results showed an antileishmanial activity (IC50: 125 μg/mL) of the obtained derivatives against L. infantum LIPA 137.
authors.affiliation: Clermont Université, Université Blaise Pascal, Institut Pascal, UMR 6602 CNRS Polytech' Clermont-Ferrand, 24 avenue des Landais, BP 206, Aubière Cedex F-63174, France. guillaume.pierre@univ-bpclermont.fr
authors.forename: Guillaume
authors.initials: G
authors.lastname: Pierre
authors.forename: Rym
authors.initials: R
authors.lastname: Salah
authors.forename: Christine
authors.initials: C
authors.lastname: Gardarin
authors.forename: Mounir
authors.initials: M
authors.lastname: Traikia
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Petit
authors.forename: Anne-Marie
authors.initials: AM
authors.lastname: Delort
authors.forename: Nabil
authors.initials: N
authors.lastname: Mameri
authors.forename: Farida
authors.initials: F
authors.lastname: Moulti-Mati
authors.forename: Philippe
authors.initials: P
authors.lastname: Michaud
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology; D000981:Antiprotozoal Agents / Q000737:chemistry / Q000494:pharmacology; D048271:Chitosan / Q000737:chemistry* / Q000494:pharmacology; D003497:Cyclic N-Oxides / Q000737:chemistry; D006868:Hydrolysis; D010084:Oxidation-Reduction
chemical_list: D000900:Anti-Bacterial Agents; D000981:Antiprotozoal Agents; D003497:Cyclic N-Oxides; D048271:Chitosan; C003959:TEMPO
keywords: Chitosan; Leishmania; TEMPO oxidation