title: Characterization and immunolocalization of mutated ornithine decarboxylase antizyme from Angiostrongylus cantonensis.
abstract: Ornithine decarboxylase antizyme (OAZ), a prominent regulator of cell proliferation, DNA/RNA transformation and tumorigenesis, can bind to ornithine decarboxylase (ODC) and facilitate its degradation. Expression of OAZ requires a unique ribosomal frame shift that is regulated by levels of polyamine in the cell. In this study, we cloned an OAZ gene with the +1 ribosomal frame-shift from a fourth-stage larvae cDNA library of Angiostrongylus cantonensis. We removed one nucleotide to express the gene without polyamine. The sequence analysis showed that the deleted-mutation ornithine decarboxylase antizyme (DM-AcOAZ) contained a conservative domain related to other species OAZ. Quantitative real-time PCR revealed that DM-AcOAZ was expressed in L3 and L4 stages and adult female worms. More notably the expression level is the highest in the adult female stage. Immunohistochemical studies indicated that DM-AcOAZ was specifically localized in the uterus, oocyte and intestine in adult female worms. MTT assays showed that in DM-AcOAZ transfected HeLa cells, cell proliferation is inhibited. In conclusion, DM-AcOAZ may be a female-enriched protein and may involved in the cell proliferation in A. cantonensis.
authors.affiliation: Department of Parasitology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, Guangdong 510080, People's Republic of China.
authors.forename: Jing
authors.initials: J
authors.lastname: Chen
authors.forename: Qian
authors.initials: Q
authors.lastname: Liu
authors.forename: Xiao
authors.initials: X
authors.lastname: Yang
authors.forename: Xiansheng
authors.initials: X
authors.lastname: Wu
authors.forename: Dongjing
authors.initials: D
authors.lastname: Zhang
authors.forename: Ai
authors.initials: A
authors.lastname: He
authors.forename: Ximei
authors.initials: X
authors.lastname: Zhan
mesh_terms: D000595:Amino Acid Sequence; D017185:Angiostrongylus cantonensis / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development*; D000818:Animals; D003001:Cloning, Molecular; D004847:Epithelial Cells / Q000469:parasitology; D005260:Female; D020869:Gene Expression Profiling; D015723:Gene Library; D006367:HeLa Cells; D006801:Humans; D007150:Immunohistochemistry; D007422:Intestines / Q000737:chemistry; D008969:Molecular Sequence Data; D009865:Oocytes / Q000737:chemistry; D011506:Proteins / Q000032:analysis* / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D014599:Uterus / Q000737:chemistry
chemical_list: D011506:Proteins; C031017:ornithine decarboxylase antizyme
keywords: Angiostrongylus cantonensis; Cell proliferation; Clone; Mutated ornithine decarboxylase antizyme