title: Optimizing selectivity during reversed-phase high performance liquid chromatography method development: prioritizing experimental conditions.
abstract: Several different conditions can be varied to improve selectivity for reversed-phase chromatography (RPC). A reexamination of literature data suggests that changes in selectivity due to a change of column or mobile phase pH are largely replicated by changes in temperature or mobile phase composition (concentrations of acetonitrile and/or methanol). This suggests a reconsideration of the role of mobile phase pH and the column during method development.
authors.affiliation: LC Resources, Walnut Creek, CA, USA.
authors.forename: Lloyd R
authors.initials: LR
authors.lastname: Snyder
authors.forename: John W
authors.initials: JW
authors.lastname: Dolan
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D056148:Chromatography, Reverse-Phase / Q000379:methods*
keywords: Experimental conditions; Experimental design; HPLC; Method development; Optimization; Selectivity