title: Magnetic isotope and magnetic field effects on the DNA synthesis.
abstract: Magnetic isotope and magnetic field effects on the rate of DNA synthesis catalysed by polymerases β with isotopic ions (24)Mg(2+), (25)Mg(2+) and (26)Mg(2+) in the catalytic sites were detected. No difference in enzymatic activity was found between polymerases β carrying (24)Mg(2+) and (26)Mg(2+) ions with spinless, non-magnetic nuclei (24)Mg and (26)Mg. However, (25)Mg(2+) ions with magnetic nucleus (25)Mg were shown to suppress enzymatic activity by two to three times with respect to the enzymatic activity of polymerases β with (24)Mg(2+) and (26)Mg(2+) ions. Such an isotopic dependence directly indicates that in the DNA synthesis magnetic mass-independent isotope effect functions. Similar effect is exhibited by polymerases β with Zn(2+) ions carrying magnetic (67)Zn and non-magnetic (64)Zn nuclei, respectively. A new, ion-radical mechanism of the DNA synthesis is suggested to explain these effects. Magnetic field dependence of the magnesium-catalysed DNA synthesis is in a perfect agreement with the proposed ion-radical mechanism. It is pointed out that the magnetic isotope and magnetic field effects may be used for medicinal purposes (trans-cranial magnetic treatment of cognitive deceases, cell proliferation, control of the cancer cells, etc).
authors.affiliation: Institute of Chemical Physics, Russian Academy of Sciences, 119991 Moscow, Russian Federation, Institute of Problems of Chemical Physics, Russian Academy of Sciences, 142432 Chernogolovka, Russian Federation, Moscow State University, 119992 Moscow, Russian Federation, Russian National Medical University, 117997 Moscow, Russian Federation and Institute of General and Inorganic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russian Federation.
authors.forename: Anatoly L
authors.initials: AL
authors.lastname: Buchachenko
authors.forename: Alexei P
authors.initials: AP
authors.lastname: Orlov
authors.forename: Dmitry A
authors.initials: DA
authors.lastname: Kuznetsov
authors.forename: Natalia N
authors.initials: NN
authors.lastname: Breslavskaya
mesh_terms: D004247:DNA / Q000096:biosynthesis*; D019951:DNA Polymerase beta / Q000378:metabolism*; D007554:Isotopes; D008274:Magnesium / Q000737:chemistry*; D060526:Magnetic Fields; D015033:Zinc Isotopes
chemical_list: D007554:Isotopes; D015033:Zinc Isotopes; D004247:DNA; D019951:DNA Polymerase beta; D008274:Magnesium