title: Site-specific, covalent incorporation of Tus, a DNA-binding protein, on ionic-complementary self-assembling peptide hydrogels using transpeptidase Sortase A as a conjugation tool†Dedicated to the memory of Joachim H. G. Steinke.‡Electronic supplementary information (ESI) available: Further experimental data. See DOI: 10.1039/c3sm00131hClick here for additional data file.
abstract: The site-specific conjugation of DNA-binding protein (Tus) to self-assembling peptide FEFEFKFKK was demonstrated. Rheology studies and TEM of the corresponding hydrogels (including PNIPAAm-containing systems) showed no significant variation in properties and hydrogel morphology compared to FEFEFKFKK. Critically, we demonstrate that Tus is accessible within the gel network displaying DNA-binding properties.
authors.affiliation: Department of Chemistry , University of Surrey , Guildford , GU2 7XH , UK . Email: g.cavalli@surrey.ac.uk ; Tel: +44 (0)1483 686837.
authors.forename: Susanna
authors.initials: S
authors.lastname: Piluso
authors.forename: Heather C
authors.initials: HC
authors.lastname: Cassell
authors.forename: Jonathan L
authors.initials: JL
authors.lastname: Gibbons
authors.forename: Thomas E
authors.initials: TE
authors.lastname: Waller
authors.forename: Nick J
authors.initials: NJ
authors.lastname: Plant
authors.forename: Aline F
authors.initials: AF
authors.lastname: Miller
authors.forename: Gabriel
authors.initials: G
authors.lastname: Cavalli