title: Characterization of a new apscaviroid from American persimmon.
abstract: A unique circular molecule of 358 nucleotides was detected in American persimmon (Diospyros virginiana L.). The molecule was graft-transmissible and had genetic characteristics of members of the genus Apscaviroid. It had the highest sequence similarity (72-73 %) to citrus viroid VI (CVd-VI) and formed a clade with CVd-VI, citrus dwarfing viroid, and apple dimple fruit viroid in a phylogenetic tree. The molecule was not detected in citrus, unlike CVd-VI, which infects citrus and persimmon, and it was genetically distant from persimmon latent viroid, which infects persimmon only. The genetic and biological features indicated that the molecule may be a member of a new apscaviroid species.
authors.affiliation: Grape and Persimmon Research Station, National Institute of Fruit Tree Science (NIFTS), National Agriculture and Food Research Organization (NARO), Akitsu, Hiroshima, 739-2494, Japan, itotaka@affrc.go.jp.
authors.forename: Takao
authors.initials: T
authors.lastname: Ito
authors.forename: Koichi
authors.initials: K
authors.lastname: Suzaki
authors.forename: Masaaki
authors.initials: M
authors.lastname: Nakano
authors.forename: Akihiko
authors.initials: A
authors.lastname: Sato
mesh_terms: D016000:Cluster Analysis; D029788:Diospyros / Q000821:virology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012313:RNA / Q000235:genetics*; D000079962:RNA, Circular; D012367:RNA, Viral / Q000235:genetics; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D014772:Viroids / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D000079962:RNA, Circular; D012367:RNA, Viral; D012313:RNA