title: Total antioxidant status value and superoxide dismutase activity in human colorectal cancer tissue depending on the stage of the disease: a pilot study.
abstract: BACKGROUND
  Colorectal cancer (CRC) is one of the most common malignant tumors in developed countries. Oxidative stress can cause damage to colonocytes by inducing cell cytokine synthesis and triggering the process of carcinogenesis.
  
  
  OBJECTIVE
  The aim of the study was to analyze relationships between: 1] total antioxidant status (TAS) value in CRC tissue and healthy colon tissue; 2] superoxide dismutase (SOD) activity in CRC tissue and healthy colon tissue; 3] TAS value in CRC tissue and the stage of colorectal cancer; and 4] SOD activity in CRC tissue and the stage of colorectal cancer.
  
  
  METHODS
  Adult patients undergoing colonic surgery for CRC were studied. The patients were divided into four groups according to the TNM Classification of Malignant Tumors. Spectrophotometric methods were used to measure TAS values and SOD activity, using diagnostic kits produced by Randox Laboratories.
  
  
  RESULTS
  The mean TAS value in CRC tissue was 0.0073 ± 0.0038 mmol/g of tissue, which was not significantly different from the mean value in healthy tissue (0.0067 ± 0.0035 mmol/g of tissue). SOD activity was 170 ± 32 U/g of tissue and was significantly higher in CRC than in healthy tissue (107 ± 25 U/g of tissue). In stages I, II and III of CRC, SOD activity as well as TAS value were increased in tumor tissue as compared to healthy tissue. Both SOD activity and TAS value markedly decreased in stage IV in contrast to the other stages of the disease.
  
  
  CONCLUSIONS
  The study results suggest that TAS and SOD monitoring could be valuable during the treatment of CRC.
authors.affiliation: Department of Medical Chemistry, Medical University of Lublin, Poland. joana.kocot@umlub.pl
authors.forename: Joanna
authors.initials: J
authors.lastname: Kocot
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Kiełczykowska
authors.forename: Wojciech
authors.initials: W
authors.lastname: Dąbrowski
authors.forename: Jacek
authors.initials: J
authors.lastname: Piłat
authors.forename: Sławomir
authors.initials: S
authors.lastname: Rudzki
authors.forename: Irena
authors.initials: I
authors.lastname: Musik
mesh_terms: D000368:Aged; D000975:Antioxidants / Q000378:metabolism*; D003106:Colon / Q000201:enzymology / Q000473:pathology; D015179:Colorectal Neoplasms / Q000201:enzymology* / Q000473:pathology*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D010865:Pilot Projects; D013482:Superoxide Dismutase / Q000378:metabolism*
chemical_list: D000975:Antioxidants; D013482:Superoxide Dismutase