title: Recent advances in the heterologous expression of microbial natural product biosynthetic pathways.
abstract: The heterologous expression of microbial natural product biosynthetic pathways coupled with advanced DNA engineering enables optimisation of product yields, functional elucidation of cryptic gene clusters, and generation of novel derivatives. This review summarises the recent advances in cloning and maintenance of natural product biosynthetic gene clusters for heterologous expression and the efforts fundamental for discovering novel natural products in the post-genomics era, with a focus on polyketide synthases (PKSs) and non-ribosomal polypeptide synthetases (NRPS).
authors.affiliation: School of Biotechnology and Biomolecular Sciences, The University of New South Wales, Sydney 2052, Australia.
authors.forename: Sarah E
authors.initials: SE
authors.lastname: Ongley
authors.forename: Xiaoying
authors.initials: X
authors.lastname: Bian
authors.forename: Brett A
authors.initials: BA
authors.lastname: Neilan
authors.forename: Rolf
authors.initials: R
authors.lastname: Müller
mesh_terms: D001688:Biological Products / Q000378:metabolism*; D053898:Biosynthetic Pathways / Q000235:genetics; D003001:Cloning, Molecular; D005818:Genetic Engineering / Q000379:methods*; D005810:Multigene Family; D010453:Peptide Synthases / Q000235:genetics / Q000378:metabolism
chemical_list: D001688:Biological Products; D010453:Peptide Synthases; C104385:non-ribosomal peptide synthase