title: The shift from low to high non-structural protein 1 expression in rotavirus-infected MA-104 cells.
abstract: A hallmark of group/species A rotavirus (RVA) replication in MA-104 cells is the logarithmic increase in viral mRNAs that occurs four-12 h post-infection. Viral protein synthesis typically lags closely behind mRNA synthesis but continues after mRNA levels plateau. However, RVA non-structural protein 1 (NSP1) is present at very low levels throughout viral replication despite showing robust protein synthesis. NSP1 has the contrasting properties of being susceptible to proteasomal degradation, but being stabilised against proteasomal degradation by viral proteins and/or viral mRNAs. We aimed to determine the kinetics of the accumulation and intracellular distribution of NSP1 in MA-104 cells infected with rhesus rotavirus (RRV). NSP1 preferentially localises to the perinuclear region of the cytoplasm of infected cells, forming abundant granules that are heterogeneous in size. Late in infection, large NSP1 granules predominate, coincident with a shift from low to high NSP1 expression levels. Our results indicate that rotavirus NSP1 is a late viral protein in MA-104 cells infected with RRV, presumably as a result of altered protein turnover.
authors.affiliation: Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Mexico DF, Mexico.
authors.forename: Laura
authors.initials: L
authors.lastname: Martínez-Álvarez
authors.forename: Carolina
authors.initials: C
authors.lastname: Piña-Vázquez
authors.forename: Wilbert
authors.initials: W
authors.lastname: Zarco
authors.forename: Luis
authors.initials: L
authors.lastname: Padilla-Noriega
mesh_terms: D000818:Animals; D036022:Capsid Proteins / Q000378:metabolism*; D002460:Cell Line; D015967:Gene Expression Regulation, Viral; D006168:Guinea Pigs; D012367:RNA, Viral / Q000235:genetics; D012401:Rotavirus / Q000378:metabolism* / Q000502:physiology; D017361:Viral Nonstructural Proteins / Q000378:metabolism*; D014779:Virus Replication
chemical_list: D036022:Capsid Proteins; D012367:RNA, Viral; C060339:VP4 protein, Rotavirus; D017361:Viral Nonstructural Proteins; C104282:nsp1 protein, Rotavirus