title: LCAT DNA shearing.
abstract: We present a novel method to fragment DNA by using lateral cavity acoustic transducers (LCATs). DNA solution is placed within a microfluidic device containing LCATs. The LCATs cause microstreaming, which fragments DNA within the solution without any need for purification or downstream processing. The LCAT-based DNA fragmentation method offers an easy-to-use, low-cost, low-energy way to fragment DNA that is amenable to integration on microfluidic platforms to further automate DNA processing. Furthermore, the LCAT microdevice requires less than 10 µL of sample, and no external equipment is needed besides a piezoelectric transducer.
authors.affiliation: 1Department of Biomedical Engineering, University of California-Irvine, Irvine, CA, USA.
authors.forename: Yuka
authors.initials: Y
authors.lastname: Okabe
authors.forename: Abraham P
authors.initials: AP
authors.lastname: Lee
mesh_terms: D004247:DNA / Q000737:chemistry*; D044085:Microfluidics / Q000379:methods*; D013048:Specimen Handling / Q000379:methods*
chemical_list: D004247:DNA
keywords: DNA shearing; LCAT; fragmentation; ultrasound