title: MicroRNA profiling of salivary adenoid cystic carcinoma: association of miR-17-92 upregulation with poor outcome.
abstract: BACKGROUND
  Salivary adenoid cystic carcinoma (ACC) is a rare relentlessly progressive malignant tumor. The molecular events associated with ACC tumorigenesis are poorly understood. Variable microRNAs (miRNA) have been correlated with tumorigenesis of several solid tumors but not in ACC. To investigate the association of miRNAs with the development and/or progression of ACC, we performed a comparative analysis of primary ACC specimens and matched normal samples and a pooled salivary gland standard and correlated the results with clinicopathologic factors and validated selected miRNAs in a separate set of 30 tumors.
  
  
  METHODS
  MiRNA array platform was used for the identification of target miRNAs and the data was subjected to informatics and statistical interrelations. The results were also collected with the MYB-NFIB fusion status and the clinicopathologic features.
  
  
  RESULTS
  Differentially dysregulated miRNAs in ACC were characterized in comparison to normal expression. No significant differences in miRNA expression were found between the MYB-NFIB fusion positive and -negative ACCs. Of the highly dysregulated miRNA in ACC, overexpression of the miR-17 and miR-20a were significantly associated with poor outcome in the screening and validation sets.
  
  
  CONCLUSIONS
  Our study indicates that the upregulation of miR-17-92 may play a role in the biology of ACC and could be potentially targeted in future therapeutic studies.
authors.affiliation: Department of Pathology, The University of Texas MD Anderson Cancer Center, Houston, Texas, United States of America.
authors.forename: Yoshitsugu
authors.initials: Y
authors.lastname: Mitani
authors.forename: Dianna B
authors.initials: DB
authors.lastname: Roberts
authors.forename: Hanadi
authors.initials: H
authors.lastname: Fatani
authors.forename: Randal S
authors.initials: RS
authors.lastname: Weber
authors.forename: Merrill S
authors.initials: MS
authors.lastname: Kies
authors.forename: Scott M
authors.initials: SM
authors.lastname: Lippman
authors.forename: Adel K
authors.initials: AK
authors.lastname: El-Naggar
mesh_terms: D063646:Carcinogenesis / Q000235:genetics; D003528:Carcinoma, Adenoid Cystic / Q000175:diagnosis* / Q000235:genetics* / Q000473:pathology; D005260:Female; D020869:Gene Expression Profiling; D050939:Gene Fusion; D020570:Genes, myb / Q000235:genetics; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D051839:NFI Transcription Factors / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis; D011379:Prognosis; D062085:RNA, Long Noncoding; D015203:Reproducibility of Results; D012468:Salivary Gland Neoplasms / Q000175:diagnosis* / Q000235:genetics* / Q000473:pathology; D016019:Survival Analysis; D015854:Up-Regulation
chemical_list: C568997:MIR17HG, human; D035683:MicroRNAs; D051839:NFI Transcription Factors; C110754:NFIB protein, human; D062085:RNA, Long Noncoding