title: [Study on BALB/c mice with EV71 infection by diverse inoculation routes].
abstract: OBJECTIVE
  To study the characteristics of EV71 JN200804 strain infection in one-day old BALBI c mjce and to establish a animal model of EV71 infection , and to provide information and technical support for the evaluation of the EV71 vaccine and antiviral medicine.
  
  
  METHODS
  One-day old BALBic mice were infected with EV71 JN200804 strain through oral( PO) ,intracranial(IC) ,intraperitoneal (IP), intramuscular (IM) routes, respectively. All mice were sacrificed at paralysis of hind limbs and collected organs for viral isolation, RT -PCR and pathological examination, and the electrophysiology were detected before sacrifice.
  
  
  RESULTS
  All mice infected through IC, IP and IM routes were paralyzed in hind limbs at 4-5 days and died at 7 days about, the hypokinesia and lethargy of mice were observed through PO routes. The viruses could be isolated and detected in the muscle from mice infected through IC, IP and IM routes and in the spinal cord through IC routes by viral isolation and RT-PCR. The neurogenic and myogenic disorders were detected by electromyography. Histopathologically, the varied pathologic changes were observed in the mouse cerebellum , spinal cord , muscle , heart, lung, liver and kidney.
  
  
  CONCLUSIONS
  EV71 JN200804 strain can infect one-day old BALBI c mice and induce paralysis of hind limbs, its animal infection model may apply to study of EV71 infection pathogenesis and antiviral medicine, and evaluation of the EV71 vaccine.
authors.affiliation: Shangdong Academy of Medical Sciences, Jinan, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Li
authors.forename: Ying-ying
authors.initials: YY
authors.lastname: Yue
authors.forename: Nan-nan
authors.initials: NN
authors.lastname: Song
authors.forename: Zhi-hui
authors.initials: ZH
authors.lastname: Li
authors.forename: Hong
authors.initials: H
authors.lastname: Meng
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D029821:Enterovirus A, Human / Q000276:immunology / Q000502:physiology*; D004769:Enterovirus Infections / Q000276:immunology / Q000821:virology*; D051379:Mice; D008807:Mice, Inbred BALB C