title: Ultra-performance liquid-chromatography with tandem mass spectrometry for rapid analysis of pharmacokinetics, biodistribution and excretion of schisandrin after oral administration of Shengmaisan.
abstract: This study aimed to investigate the in vivo behaviors of the main components in traditional Chinese medicine (TCM) fomulae. The plasma pharmacokinetics, tissue distribution and excretion of the main component-schisandrin in rats after oral administration of a classical TCM prescription, shengmaisan (SMS), were studied by a developed and validated UPLC-MS/MS method. The separation of schisandrin was achieved on a UPLC HSS T3 column with a mobile phase consisting of acetonitrile and water at a flow rate of 0.5 mL/min by linear gradient elution. The MS/MS detection was carried out by monitoring the fragmentation of m/z 415.22 → 384.26 for schisandrin on a triple quadrupole mass spectrometer. The result showed that the method was suitable for the quantification of schisandrin in plasma, tissue and excreta samples with satisfactory selectivity, precision, accuracy, sensitivity, linearity and recovery. Pharmacokinetic results showed a rapid absorption phase with the mean Tmax of 0.17 h and a relatively slow elimination proceeding with a half-life (T1/2 ) of 5.24 ± 1.28 h. The tissue distribution showed the maximum concentration distributions of schisandrin after oral administration of SMS were in the order of small intestine > large intestine > lung > liver > kidney > spleen > heart > brain. Only 0.005-0.006% of schisandrin was recovered in feces and was not detected in urine.
authors.affiliation: National TCM Key Laboratory of Serum Pharmacochemistry, Key Lab of Chinmedomics, Department of Pharmaceutical Analysis, Heilongjiang University of Chinese Medicine, Heping Road 24, Harbin, 150040, China.
authors.forename: Sheng-Wen
authors.initials: SW
authors.lastname: Lu
authors.forename: Ai-Hua
authors.initials: AH
authors.lastname: Zhang
authors.forename: Hui
authors.initials: H
authors.lastname: Sun
authors.forename: Guang-Li
authors.initials: GL
authors.lastname: Yan
authors.forename: Ying
authors.initials: Y
authors.lastname: Han
authors.forename: Xiu-Hong
authors.initials: XH
authors.lastname: Wu
authors.forename: Xi-Jun
authors.initials: XJ
authors.lastname: Wang
mesh_terms: D000284:Administration, Oral; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D034242:Cyclooctanes / Q000032:analysis* / Q000493:pharmacokinetics*; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000493:pharmacokinetics*; D005243:Feces / Q000737:chemistry; D017705:Lignans / Q000032:analysis* / Q000493:pharmacokinetics*; D008297:Male; D009928:Organ Specificity; D011083:Polycyclic Compounds / Q000032:analysis* / Q000493:pharmacokinetics*; D051381:Rats; D017208:Rats, Wistar; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D034242:Cyclooctanes; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D017705:Lignans; D011083:Polycyclic Compounds; C009252:fructus schizandrae, radix ginseng, radix ophiopogonis drug combination; C011105:schizandrin
keywords: UPLC-MS/MS; distribution; excretion; pharmacokinetics; schisandrin