title: Glutathione modified gold nanorods with excellent biocompatibility and weak protein adsorption, targeting imaging and therapy toward tumor cells.
abstract: The non-specific interaction between the surface of gold nanorods (GNRs) and biomolecules is a significant problem in hyperthermia applications. In this work, a novel surface modification strategy was performed using zwitterionic oxidized glutathione (GSSG) to modify the GNRs through robust Au-S bond formation. The GSSG-GNRs were stable in aqueous solution over a broad range of pH and high ionic strength values. Importantly, the glutathione coating greatly reduced non-specific protein adsorption on GNRs due to zwitterions. In order to target tumor sites, folic acid (FA) and Rhodamine B (RBITC) dye were conjugated to GSSG modified GNRs through the reaction between the activated carboxyl group and the amino group. The targeting of RBITC-FA-GSSG-GNRs in human cervical carcinoma cells (HeLa cells) with high-level folate receptor expression was confirmed using confocal laser scanning microscopy (CLSM) to excite Rhodamine B dye at 488 nm. The targeting photothermal therapy efficacy was monitored in real time with the multi-channel function of a confocal laser scanning microscope coupled with an 808 nm laser. The results indicate that the FA-GSSG-GNR hybrids demonstrate efficient photothermal conversion and excellent targeting hyperthermia, and are therefore suitable for biomedical applications.
authors.affiliation: State Key Laboratory on Integrated Optoelectronics, College of Electronic Science and Engineering, Jilin University, Changchun, 130012, People's Republic of China.
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.forename: Biao
authors.initials: B
authors.lastname: Dong
authors.forename: Boting
authors.initials: B
authors.lastname: Chen
authors.forename: Sai
authors.initials: S
authors.lastname: Xu
authors.forename: Shuang
authors.initials: S
authors.lastname: Zhang
authors.forename: Wei
authors.initials: W
authors.lastname: Yu
authors.forename: Chunxiang
authors.initials: C
authors.lastname: Xu
authors.forename: Hongwei
authors.initials: H
authors.lastname: Song
mesh_terms: D055011:Ablation Techniques; D000327:Adsorption; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology*; D002417:Cattle; D045744:Cell Line, Tumor; D005492:Folic Acid / Q000737:chemistry; D019803:Glutathione Disulfide / Q000737:chemistry*; D006046:Gold / Q000737:chemistry*; D006801:Humans; D057054:Molecular Imaging / Q000379:methods*; D043942:Nanotubes / Q000737:chemistry*; D012235:Rhodamines / Q000737:chemistry; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D001672:Biocompatible Materials; D012235:Rhodamines; D012710:Serum Albumin, Bovine; D006046:Gold; D005492:Folic Acid; C029773:rhodamine B; D019803:Glutathione Disulfide