title: Cross-Coupling Modification of Nucleoside Triphosphates, PEX, and PCR Construction of Base-Modified DNA.
abstract: A novel, efficient, two-step methodology is presented for construction of base-modified oligonucleotides or DNA, involving aqueous cross-coupling reactions of halogenated nucleoside triphosphates (dNTPs) with terminal acetylenes or arylboronic acids, followed by polymerase incorporation of the modified dNTPs either using primer extension (PEX) or polymerase chain reaction (PCR). Curr. Protoc. Chem Biol. 2:1-14. © 2010 by John Wiley & Sons, Inc.
authors.affiliation: Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, Prague, Czech Republic.
authors.forename: Hana
authors.initials: H
authors.lastname: Macickova-Cahová
authors.forename: Milan
authors.initials: M
authors.lastname: Vrábel
authors.forename: Michal
authors.initials: M
authors.lastname: Hocek
keywords: DNA polymerase; base‐modified DNA; cross‐coupling; nucleoside triphosphates