title: A virus-induced gene silencing method to study soybean cyst nematode parasitism in Glycine max.
abstract: BACKGROUND
  Bean pod mottle virus (BPMV) based virus-induced gene silencing (VIGS) vectors have been developed and used in soybean for the functional analysis of genes involved in disease resistance to foliar pathogens. However, BPMV-VIGS protocols for studying genes involved in disease resistance or symbiotic associations with root microbes have not been developed.
  
  
  RESULTS
  Here we describe a BPMV-VIGS protocol suitable for reverse genetic studies in soybean roots. We use this method for analyzing soybean genes involved in resistance to soybean cyst nematode (SCN). A detailed SCN screening pipeline is described.
  
  
  CONCLUSIONS
  The VIGS method described here provides a new tool to identify genes involved in soybean-nematode interactions. This method could be adapted to study genes associated with any root pathogenic or symbiotic associations.
authors.affiliation: Division of Plant Sciences, Christopher S, Bond Life Sciences Center and Interdisciplinary Plant Group, University of Missouri, Columbia, MO 65211, USA.
authors.forename: Pramod K
authors.initials: PK
authors.lastname: Kandoth
authors.forename: Robert
authors.initials: R
authors.lastname: Heinz
authors.forename: Greg
authors.initials: G
authors.lastname: Yeckel
authors.forename: Nathan W
authors.initials: NW
authors.lastname: Gross
authors.forename: Parijat S
authors.initials: PS
authors.lastname: Juvale
authors.forename: John
authors.initials: J
authors.lastname: Hill
authors.forename: Steven A
authors.initials: SA
authors.lastname: Whitham
authors.forename: Thomas J
authors.initials: TJ
authors.lastname: Baum
authors.forename: Melissa G
authors.initials: MG
authors.lastname: Mitchum
mesh_terms: D000818:Animals; D017798:Comovirus / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant; D020868:Gene Silencing; D005822:Genetic Vectors; D006790:Host-Parasite Interactions / Q000235:genetics; D009348:Nematoda / Q000235:genetics*; D018517:Plant Roots; D030821:Plants, Genetically Modified / Q000235:genetics* / Q000469:parasitology*; D034622:RNA Interference; D013025:Glycine max / Q000235:genetics* / Q000469:parasitology*