title: Scorpion venom peptide SPVII promotes irradiated cells proliferation and increases the expression of the IL-3 receptor.
abstract: BACKGROUND
  The previous investigation demonstrated the radioprotective efficacy of peptides isolated from the venom of Buthus Martti Karsch. In this study, the effect of isolated scorpion venom peptide II (SVPII) on irradiated M-NFS-60 cells and mouse bone marrow mononuclear cells (BM-MNCs) was observed. The AlamarBlue cell viability assay, a colony-forming unit (CFU) assay, flow cytometry (FCM), immunofluorescence, and Western blotting were used to evaluate cell proliferation, cell cycle progression, and the expression of the IL-3 receptor (IL-3R) protein in non-irradiated and irradiated cells.
  
  
  RESULTS
  Proliferation of irradiated M-NFS-60 cells was significantly accelerated by SPVII, and this effect was further enhanced by co-application of IL-3. Similarly, SPVII increased the number of BM-MNC CFUs and this proliferative effect was greater in the presence of SVPII plus IL-3. In addition, SPVII significantly altered cell cycle progression; SVPII enhanced the fraction of unirradiated M-NFS-60 cells in S phase and the fraction of irradiated M-NFS-60 cells arrested in G2/M. The expression of IL-3R protein by unirradiated M-NFS-60 cells was enhanced significantly by SVPII, and SVPII-induced IL-3R overexpression was 10-fold greater in irradiated M-NFS-60 cells.
  
  
  CONCLUSIONS
  These results indicated the hematopoietic growth factor (HGF)-like effects of SVPII on irradiated cells, possibly mediated by upregulation of IL-3R.
authors.affiliation: Department of Pathophysiology, Guangzhou Medical College, Guangzhou 510182, Guangdong, China. tianhankong@163.com.
authors.forename: Yifang
authors.initials: Y
authors.lastname: Qiu
authors.forename: Liyuan
authors.initials: L
authors.lastname: Jiang
authors.forename: Caixia
authors.initials: C
authors.lastname: Wang
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.forename: Ting
authors.initials: T
authors.lastname: Li
authors.forename: Baiqian
authors.initials: B
authors.lastname: Xing
authors.forename: Meixun
authors.initials: M
authors.lastname: Zhou
authors.forename: Tianhan
authors.initials: T
authors.lastname: Kong
authors.forename: Weihua
authors.initials: W
authors.lastname: Dong