title: Antibacterial, cytotoxicity and physical properties of laser--silver doped hydroxyapatite layers.
abstract: Hydroxyapatite layers with silver doping from 0.06 at.% to 14 at.% were prepared by laser deposition. The films' physical properties such as morphology, composition, crystallinity, Young's modulus and microhardness were measured. Films were amorphous or polycrystalline in dependence on deposition temperature (from RT to 600 °C). Antibacterial properties were tested using Escherichia coli and Bacillus subtilis cells. The antibacterial efficacy changed with silver doping from 4% to 100%. Cytotoxicity was studied by a direct contact test. Depending on doping and crystallinity the films were either non-toxic or mildly toxic.
authors.affiliation: Institute of Physics ASCR v.v.i., Na Slovance 2, 18221 Prague 8, Czech Republic. jelinek@fzu.cz
authors.forename: M
authors.initials: M
authors.lastname: Jelinek
authors.forename: T
authors.initials: T
authors.lastname: Kocourek
authors.forename: J
authors.initials: J
authors.lastname: Remsa
authors.forename: M
authors.initials: M
authors.lastname: Weiserová
authors.forename: K
authors.initials: K
authors.lastname: Jurek
authors.forename: J
authors.initials: J
authors.lastname: Mikšovský
authors.forename: J
authors.initials: J
authors.lastname: Strnad
authors.forename: A
authors.initials: A
authors.lastname: Galandáková
authors.forename: J
authors.initials: J
authors.lastname: Ulrichová
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D016923:Cell Death / Q000187:drug effects; D003460:Crystallization; D017886:Durapatite / Q000494:pharmacology*; D055119:Elastic Modulus / Q000187:drug effects; D004926:Escherichia coli / Q000187:drug effects; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects; D006244:Hardness / Q000187:drug effects; D007834:Lasers; D051379:Mice; D008826:Microbial Sensitivity Tests; D008855:Microscopy, Electron, Scanning; D041681:NIH 3T3 Cells; D012825:Silicon / Q000494:pharmacology; D013211:Staphylococcus aureus / Q000187:drug effects; D014025:Titanium / Q000494:pharmacology; D014961:X-Ray Diffraction
chemical_list: D000900:Anti-Bacterial Agents; D017886:Durapatite; D014025:Titanium; D012825:Silicon
keywords: Antibacterial; Cytotoxicity; Hydroxyapatite; PLD; Silver; Thin films