title: 13²,17³-Cyclopheophorbide b enol as a catabolite of chlorophyll b in phycophagy by protists.
abstract: Both 13(2),17(3)-cyclopheophorbide a and b enols were produced along with ingestion of green microalgae containing chlorophylls a and b by a centrohelid protist (phycophagy). The results suggest that chlorophyll b as well as chlorophyll a were directly degraded to colored yet non-phototoxic catabolites in the protistan phycophagic process. Such a simple process by the predators makes a contrast to the much sophisticated chlorophyll degradation process of land plants and some algae, where phototoxicity of chlorophylls was cancelled through the multiple enzymatic steps resulting in colorless and non-phototoxic catabolites.
authors.affiliation: Graduate School of Life Sciences, Ritsumeikan University, Kusatsu, Shiga 525-8577, Japan. chiro@fukui-ut.ac.jp
authors.forename: Yuichiro
authors.initials: Y
authors.lastname: Kashiyama
authors.forename: Akiko
authors.initials: A
authors.lastname: Yokoyama
authors.forename: Takashi
authors.initials: T
authors.lastname: Shiratori
authors.forename: Isao
authors.initials: I
authors.lastname: Inouye
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Kinoshita
authors.forename: Tadashi
authors.initials: T
authors.lastname: Mizoguchi
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Tamiaki
mesh_terms: D002734:Chlorophyll / Q000031:analogs & derivatives* / Q000737:chemistry; D000460:Chlorophyta / Q000737:chemistry*; D018920:Coculture Techniques; D058086:Microalgae / Q000737:chemistry*; D008956:Models, Chemical; D010100:Oxygen / Q000378:metabolism; D012623:Seawater
chemical_list: C583896:13(2),17(3)-cyclopheophorbide b enol; D002734:Chlorophyll; C037184:chlorophyll b; D010100:Oxygen
keywords: (13(2)R)- and (13(2)S)-hydroxychlorophyllones; (R/S)-hCPLs; 13(2),17(3)-cyclopheophorbide a enol; 13(2),17(3)-cyclopheophorbide b enol; APCI; Aquatic ecosystem; Chl; Chlorophyll catabolism; Cyclopheophorbide enol; Microalga; PAO; PDA; PPB; Phe; Phycophagy; Protist; TOF; atmospheric pressure chemical ionization; cPPB-aE; cPPB-bE; chlorophyll; pPPB; pPhe; pheophorbide; pheophorbide a oxygenase; pheophytin; photodiode array; pyropheophorbide; pyropheophytin; time-of-flight