title: Site-selective adsorption of protein induced by a metal pattern on a poly(ethylene terephthalate) surface.
abstract: A novel technique for inducing site-selective adsorption of protein through constructing metal patterns on flexible poly(ethylene terephthalate) surfaces is presented. The substrates were first modified by vacuum ultraviolet (VUV) irradiation through a photomask to introduce regions with different functional groups. Then the designed metal patterns were constructed on the surfaces of VUV-treated substrates. The surface rearrangement was effectively prevented by constructing silver patterns on poly(ethylene terephthalate) surfaces, thus significantly improving the stability and selectivity of protein adsorption on the surfaces. Moreover, the protein-repulsive layer further reinforced the effect. Finally, protein patterns were successfully obtained. As confirmed by fluorescence microscope, field emission scanning electron microscopy (FE-SEM), X-ray photoelectron spectroscopy (XPS), and static water contact angle measurement, the protein patterns possess both excellent selectivity and high fidelity. Feature size of the protein patterns surrounded by a protein-repulsive layer was exactly the same as that of the photomask. And the grain sizes of silver particles were approximately 50 nm. This work could potentially be used in various fields such as biomedicine, bioelectronic components, and tissue repair and replacement, where selective adsorption of protein is desired.
authors.affiliation: School of Materials Science and Engineering, Wuhan University of Technology, 122 Luoshi Road, Wuhan 430070, China.
authors.forename: Jiali
authors.initials: J
authors.lastname: Cao
authors.affiliation: School of Materials Science and Engineering, Wuhan University of Technology, 122 Luoshi Road, Wuhan 430070, China. Electronic address: zkwu@whut.edu.cn.
authors.forename: Zhongkui
authors.initials: Z
authors.lastname: Wu
authors.affiliation: School of Materials Science and Engineering, Wuhan University of Technology, 122 Luoshi Road, Wuhan 430070, China.
authors.forename: Shaoying
authors.initials: S
authors.lastname: Li
authors.affiliation: School of Materials Science and Engineering, Wuhan University of Technology, 122 Luoshi Road, Wuhan 430070, China.
authors.forename: Hongxiao
authors.initials: H
authors.lastname: Tang
authors.affiliation: School of Materials Science and Engineering, Wuhan University of Technology, 122 Luoshi Road, Wuhan 430070, China.
authors.forename: Qilin
authors.initials: Q
authors.lastname: Mei
mesh_terms: D000327:Adsorption; D000818:Animals; D002417:Cattle; D003300:Copper / Q000737:chemistry*; D008856:Microscopy, Fluorescence; D056951:Photoelectron Spectroscopy; D011093:Polyethylene Terephthalates / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D013499:Surface Properties; D014867:Water / Q000737:chemistry
chemical_list: D011093:Polyethylene Terephthalates; D014867:Water; D012710:Serum Albumin, Bovine; D003300:Copper
keywords: Flexible substrate; Metal pattern; Protein adsorption; Protein-repulsive layer; Site-selective