title: Biomineral proteins from Mytilus edulis mantle tissue transcriptome.
abstract: The common blue mussel, Mytilus edulis, has a bimineralic shell composed of approximately equal proportions of the two major polymorphs of calcium carbonate: calcite and aragonite. The exquisite biological control of polymorph production is the focus of research interest in terms of understanding the details of biomineralisation and the proteins involved in the process of complex shell formation. Recent advances in ease and availability of pyrosequencing and assembly have resulted in a sharp increase in transcriptome data for invertebrate biominerals. We have applied Roche 454 pyrosequencing technology to profile the transcriptome for the mantle tissue of the bivalve M. edulis. A comparison was made between the results of several assembly programs: Roche Newbler assembler versions 2.3, 2.5.2 and 2.6 and MIRA 3.2.1 and 3.4.0. The Newbler and MIRA assemblies were subsequently merged using the CAP3 assembler to give a higher consensus in alignments and a more accurate estimate of the true size of the M. edulis transcriptome. Comparison sequence searches show that the mantle transcripts for M. edulis encode putative proteins exhibiting sequence similarities with previously characterised shell proteins of other species of Mytilus, the Bivalvia Pinctada and haliotid gastropods. Importantly, this enhanced transcriptome has detected several transcripts that encode proteins with sequence similarity with previously described shell biomineral proteins including Shematrins and lysine-rich matrix proteins (KRMPs) not previously found in Mytilus.
authors.affiliation: School of Chemistry, University of Glasgow, G12 8QQ, Glasgow, UK.
authors.forename: Andy
authors.initials: A
authors.lastname: Freer
authors.forename: Stephen
authors.initials: S
authors.lastname: Bridgett
authors.forename: Jiahong
authors.initials: J
authors.lastname: Jiang
authors.forename: Maggie
authors.initials: M
authors.lastname: Cusack
mesh_terms: D060105:Animal Shells / Q000737:chemistry* / Q000378:metabolism; D000825:Animal Structures / Q000378:metabolism*; D000818:Animals; D001483:Base Sequence; D058977:Molecular Sequence Annotation; D008969:Molecular Sequence Data; D049880:Mytilus edulis / Q000235:genetics* / Q000378:metabolism; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D016415:Sequence Alignment; D017423:Sequence Analysis, RNA; D012984:Software; D059467:Transcriptome / Q000235:genetics*
chemical_list: D011506:Proteins