title: Free radical scavenging and anti-oxidative activities of an ethanol-soluble pigment extract prepared from fermented Zijuan Pu-erh tea.
abstract: An ethanol-soluble pigment extract was separated from fermented Zijuan Pu-erh tea. The compositions of the ethanol soluble pigment extract were analyzed by high-performance liquid chromatography-tandem mass spectroscopy (HPLC-MS/MS). The extract was prepared into a series of ethanol solutions and analyzed for free radical-scavenging activities (against two free radicals: 1,1-diphenyl-2-picrylhydrazyl (DPPH) and (2,2,6,6-tetramethylpiperidin-1-yl)oxyl (TEMPO)) and in vitro anti-oxidative properties. Electron spin resonance spectroscopy showed that the peaks of DPPH and TEMPO decreased with increasing extract concentration, suggesting that the extract had excellent free radical-scavenging activities. In vitro cell culture suggested that, at 50-200 mg/L, the extract had no measurable effect on the viability of vascular endothelial cells (ECV340) but produced significant protective effects for cells that underwent oxidative injuries due to hydrogen peroxide (H₂O₂) treatment. Compared with the H₂O₂ treatment alone cells group, 200 mg/L of the extract increased the activity of superoxide dismutase (SOD) in cells by 397.3%, and decreased the concentration of malondialdehyde (MDA) and the activity of lactate acid dehydrogenase (LDH) by 47.8% and 69.6%, respectively. These results suggest that the extract has excellent free radical scavenging and anti-oxidative properties.
authors.affiliation: College of Food Science and Technology, Yunnan Agricultural University, Kunming 650201, China.
authors.forename: Jiang Ping
authors.initials: JP
authors.lastname: Fan
authors.forename: Chong
authors.initials: C
authors.lastname: Fan
authors.forename: Wen Min
authors.initials: WM
authors.lastname: Dong
authors.forename: Bin
authors.initials: B
authors.lastname: Gao
authors.forename: Wei
authors.initials: W
authors.lastname: Yuan
authors.forename: Jia Shun
authors.initials: JS
authors.lastname: Gong
mesh_terms: D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*; D028241:Camellia sinensis / Q000737:chemistry / Q000378:metabolism; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D002681:China; D004032:Diet / Q000208:ethnology; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D000431:Ethanol / Q000737:chemistry; D020834:Ethnopharmacology; D005285:Fermentation; D005511:Food Handling; D016166:Free Radical Scavengers / Q000009:adverse effects / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D006801:Humans; D045022:Lactate Dehydrogenases / Q000737:chemistry / Q000378:metabolism*; D008315:Malondialdehyde / Q000037:antagonists & inhibitors / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D010860:Pigments, Biological / Q000009:adverse effects / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D010936:Plant Extracts / Q000009:adverse effects / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D012995:Solubility; D012997:Solvents / Q000737:chemistry; D013482:Superoxide Dismutase / Q000737:chemistry / Q000378:metabolism*; D013662:Tea / Q000737:chemistry* / Q000382:microbiology
chemical_list: D000975:Antioxidants; D016166:Free Radical Scavengers; D010860:Pigments, Biological; D010936:Plant Extracts; D012997:Solvents; D013662:Tea; D000431:Ethanol; D008315:Malondialdehyde; D045022:Lactate Dehydrogenases; D013482:Superoxide Dismutase
keywords: Anti-oxidation; Ethanol-soluble pigment; Free radical; Zijuan Pu-erh tea