title: WWP1 gene is a potential molecular target of human oral cancer.
abstract: OBJECTIVE
  This study investigates the oncogenic role of WWP1, an ubiquitin ligase linked to tumor promotion, in oral cancer.
  
  
  METHODS
  An array-based comparative genomic hybridization was used to detect chromosomal changes in 10 oral cancer specimens. An additional 59 specimens and 6 cultured oral cancer cells were further examined to evaluate changes in the DNA copy number and messenger RNA (mRNA) and protein expression of WWP1.
  
  
  RESULTS
  The copy number of the WWP1 gene and its mRNA levels were significantly increased in the oral cancer specimens. An elevated WWP1 gene expression was observed in 6 cultured oral cancer cell lines. Knockdown of the endogenous WWP1 using small hairpin RNA further showed that deficiency of WWP1 suppressed cell growth and caused apoptosis in oral cancer cells.
  
  
  CONCLUSIONS
  Our results reveal that WWP1 might play an oncogenic role in oral cancer cells.
authors.affiliation: Department of Biological Science and Technology, China Medical University, Taichung, Taiwan. linjh@mail.cmu.edu.tw
authors.forename: Ju-Hwa
authors.initials: JH
authors.lastname: Lin
authors.forename: Shu-Chen
authors.initials: SC
authors.lastname: Hsieh
authors.forename: Jia-Ni
authors.initials: JN
authors.lastname: Chen
authors.forename: Ming-Hsui
authors.initials: MH
authors.lastname: Tsai
authors.forename: Chia-Che
authors.initials: CC
authors.lastname: Chang
mesh_terms: D000328:Adult; D000368:Aged; D017209:Apoptosis / Q000235:genetics; D063646:Carcinogenesis / Q000235:genetics; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D055028:Comparative Genomic Hybridization; D056915:DNA Copy Number Variations / Q000235:genetics; D005091:Exons / Q000235:genetics; D005784:Gene Amplification / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D055785:Gene Knockdown Techniques; D018390:Gene Targeting; D006801:Humans; D008297:Male; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000235:genetics* / Q000473:pathology; D009154:Mutation / Q000235:genetics; D009367:Neoplasm Staging; D009857:Oncogenes / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D034741:RNA, Small Interfering / Q000235:genetics; D044767:Ubiquitin-Protein Ligases / Q000235:genetics*
chemical_list: D012333:RNA, Messenger; D034741:RNA, Small Interfering; C471642:WWP1 protein, human; D044767:Ubiquitin-Protein Ligases