title: Albendazole and its derivative JVG9 induce encystation on Giardia intestinalis trophozoites.
abstract: In the present study, we evaluated the effect of an albendazole (ABZ) derivative JVG9 on cultured Giardia intestinalis. To assess the JVG9 effects, we evaluated the tubulin cytoskeleton by confocal microscopy, and we found that the characteristic staining was modified. The scanning electron microscopy images revealed extremely damaged trophozoites and cyst-like cells. The confocal images revealed that this drug triggered the expression of cyst wall protein 1 and encystation. We also found that at low doses, AL triggered the encystation process too.
authors.affiliation: Departamento de Biología Celular, Centro de Investigación y Estudios Avanzados del Instituto Politécnico Nacional, Av. IPN 2508, Col. San Pedro Zacatenco, 07360, México, DF Mexico.
authors.forename: Armando
authors.initials: A
authors.lastname: Pérez-Rangel
authors.forename: José Manuel
authors.initials: JM
authors.lastname: Hernández
authors.forename: Araceli
authors.initials: A
authors.lastname: Castillo-Romero
authors.forename: Lilián
authors.initials: L
authors.lastname: Yépez-Mulia
authors.forename: Rafael
authors.initials: R
authors.lastname: Castillo
authors.forename: Francisco
authors.initials: F
authors.lastname: Hernández-Luis
authors.forename: Benjamín
authors.initials: B
authors.lastname: Nogueda-Torres
authors.forename: Juan Pedro
authors.initials: JP
authors.lastname: Luna-Arias
authors.forename: Gerardo
authors.initials: G
authors.lastname: Radilla
authors.forename: Gloria
authors.initials: G
authors.lastname: León-Avila
mesh_terms: D015766:Albendazole / Q000031:analogs & derivatives / Q000494:pharmacology*; D000818:Animals; D000871:Anthelmintics / Q000494:pharmacology*; D000913:Antibodies, Protozoan / Q000276:immunology; D005786:Gene Expression Regulation; D005872:Giardia / Q000187:drug effects* / Q000254:growth & development / Q000276:immunology / Q000648:ultrastructure; D051379:Mice; D008807:Mice, Inbred BALB C; D018613:Microscopy, Confocal; D008855:Microscopy, Electron, Scanning; D015800:Protozoan Proteins / Q000276:immunology / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D011994:Recombinant Proteins; D053058:Trophozoites / Q000187:drug effects / Q000254:growth & development / Q000276:immunology / Q000648:ultrastructure; D014404:Tubulin / Q000378:metabolism
chemical_list: D000871:Anthelmintics; D000913:Antibodies, Protozoan; C094333:CWP1 protein, Giardia lamblia; D015800:Protozoan Proteins; D011994:Recombinant Proteins; D014404:Tubulin; D015766:Albendazole