title: Differential peroxidase activities in three different crops upon insect feeding.
abstract: Peroxidases are the ubiquitous enzyme and reported to be present in all living genera. They catalyses reduction of peroxide and generate reactive oxygen species. In the present study we demonstrated that insect infestation induces peroxidase activity in sap and total soluble protein (TSP) of plant leaves. Three important crop plants viz. tomato, cowpea and cotton were used for this study. After infestation of chewing insect, Peroxidase activity in the sap and TSP of all the studied plants were enhanced in the range of 1.6 to 3.14 fold. Similar observations were also obtained with feeding of sap sucking insects, in which increment in peroxidase activity of sap and TSP was in the range of 1.8 to 2.53 fold. Enhanced peroxidase activity was reconfirmed by in-gel peroxidase assay. Enzyme kinetic study showed turn over efficiency of peroxidase from cotton (~101.3 min(-1)) was almost similar to tomato (~100.8 min(-1)) but higher than cowpea (~98.21 in(-1)). MS/MS analysis of observed band showed significant similarity with the reported peroxidases in database.
authors.affiliation: CSIR-National Botanical Research Institute; Council of Scientific and Industrial Research; Lucknow, UP, India.
authors.forename: Harpal
authors.initials: H
authors.lastname: Singh
authors.forename: Sameer
authors.initials: S
authors.lastname: Dixit
authors.forename: Pradhyumna
authors.initials: P
authors.lastname: Singh
authors.forename: Praveen C
authors.initials: PC
authors.lastname: Verma
mesh_terms: D000818:Animals; D018556:Crops, Agricultural / Q000469:parasitology*; D060434:Herbivory; D007313:Insecta / Q000502:physiology*; D007700:Kinetics; D013058:Mass Spectrometry; D009195:Peroxidase / Q000378:metabolism*; D053147:Plant Exudates / Q000378:metabolism; D018515:Plant Leaves / Q000201:enzymology; D010940:Plant Proteins / Q000378:metabolism*; D012995:Solubility
chemical_list: D053147:Plant Exudates; D010940:Plant Proteins; D009195:Peroxidase