title: [Properties of Bacillus pumilus subtilisin like proteinase secreted from recombinant strain on different growth stages].
abstract: Bacillus pumilus 3-19 glutamylendopeptidase has been isolated from culture liquid of Bacillus subtilis recombinant strain on different growth stages: growth retardation (early enzyme) and stationary phase (late enzyme). The effect of purified proteinase of different growth stages on insulin beta-chain, protein and oligopeptide substrates has been studied. Comparative study of physicochemical properties of early and late proteinases was carried out. Two protein fractions were different in catalytic characteristics and demonstrated different sensitivity to the presence of metal cations.
authors.forename: N P
authors.initials: NP
authors.lastname: Balaban
authors.forename: Iu V
authors.initials: IuV
authors.lastname: Danilova
authors.forename: T R
authors.initials: TR
authors.lastname: Shamsutdinov
authors.forename: A M
authors.initials: AM
authors.lastname: Mardanova
authors.forename: A M
authors.initials: AM
authors.lastname: Cheremin
authors.forename: G N
authors.initials: GN
authors.lastname: Rudenskaia
authors.forename: M R
authors.initials: MR
authors.lastname: Sharipova
mesh_terms: D000595:Amino Acid Sequence; D001407:Bacillus / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D002384:Catalysis; D002412:Cations / Q000737:chemistry; D007328:Insulin / Q000737:chemistry; D008670:Metals / Q000737:chemistry; D008969:Molecular Sequence Data; D012697:Serine Endopeptidases / Q000302:isolation & purification* / Q000378:metabolism; D013379:Substrate Specificity; D020860:Subtilisin / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism
chemical_list: D002412:Cations; D007328:Insulin; D008670:Metals; D012697:Serine Endopeptidases; C028344:glutamyl endopeptidase; D020860:Subtilisin