title: Label-free CMOS bio sensor with on-chip noise reduction scheme for real-time quantitative monitoring of biomolecules.
abstract: We present a label-free CMOS field-effect transistor sensing array to detect the surface potential change affected by the negative charge in DNA molecules for real-time monitoring and quantification. The proposed CMOS bio sensor includes a new sensing pixel architecture implemented with correlated double sampling for reducing offset fixed pattern noise and 1/f noise of the sensing devices. We incorporated non-surface binding detection which allows real-time continuous monitoring of DNA concentrations without immobilizing them on the sensing surface. Various concentrations of 19-bp oligonucleotides solution can be discriminated using the prototype device fabricated in 1- μm double-poly double-metal standard CMOS process. The detection limit was measured as 1.1 ng/μl with a dynamic range of 40 dB and the transient response time was measured less than 20 seconds.
authors.affiliation: Department of Electrical and Computer Engineering, University of Minnesota, Minneapolis, MN 55455 USA. lucid97@gmail.com
authors.lastname: Seong-Jin Kim
authors.lastname: Euisik Yoon
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation*; D004247:DNA / Q000032:analysis*; D004563:Electrochemistry; D004566:Electrodes; D004581:Electronics; D004867:Equipment Design; D006863:Hydrogen-Ion Concentration; D056656:Lab-On-A-Chip Devices; D008670:Metals / Q000737:chemistry; D046210:Microfluidic Analytical Techniques; D044085:Microfluidics; D008904:Miniaturization; D009841:Oligonucleotides / Q000032:analysis; D012666:Semiconductors; D012815:Signal Processing, Computer-Assisted; D013499:Surface Properties; D014173:Transistors, Electronic
chemical_list: D008670:Metals; D009841:Oligonucleotides; D004247:DNA