title: Topography of distinct Staphylococcus aureus types in chronic wounds of patients with epidermolysis bullosa.
abstract: The opportunistic pathogen Staphylococcus aureus is known to interfere with wound healing and represents a significant risk factor for wound infections and invasive disease. It is generally assumed that one individual is predominantly colonized by one S. aureus type. Nevertheless, patients with the genetic blistering disease epidermolysis bullosa (EB) often carry multiple S. aureus types. We therefore investigated whether different S. aureus types are present in individual wounds of EB patients and, if so, how they are spatially distributed. The staphylococcal topography in chronic wounds was mapped by replica-plating of used bandages and subsequent typing of S. aureus isolates. Individual chronic wounds of five patients contained up to six different S. aureus types. Unexpectedly, distinct S. aureus types formed micro-colonies that were located in close proximity and sometimes even overlapped. While some adjacent S. aureus isolates were closely related, others belonged to distinct molecular complexes. We conclude that the general assumption that one individual is predominantly colonized by one type of S. aureus does not apply to chronic wounds of EB patients. We consider this observation important, not only for EB patients, but also for other patients with chronic wounds in view of the potential risk for severe staphylococcal infections.
authors.affiliation: Department of Medical Microbiology, University of Groningen and University Medical Center Groningen, Groningen, The Netherlands.
authors.forename: Magdalena M
authors.initials: MM
authors.lastname: van der Kooi-Pol
authors.forename: Mehdi
authors.initials: M
authors.lastname: Sadaghian Sadabad
authors.forename: José C
authors.initials: JC
authors.lastname: Duipmans
authors.forename: Artur J
authors.initials: AJ
authors.lastname: Sabat
authors.forename: Tim
authors.initials: T
authors.lastname: Stobernack
authors.forename: Till F
authors.initials: TF
authors.lastname: Omansen
authors.forename: Gerlinde N
authors.initials: GN
authors.lastname: Westerhout-Pluister
authors.forename: Marcel F
authors.initials: MF
authors.lastname: Jonkman
authors.forename: Hermie J M
authors.initials: HJ
authors.lastname: Harmsen
authors.forename: Jan Maarten
authors.initials: JM
authors.lastname: van Dijl
mesh_terms: D001458:Bandages / Q000382:microbiology; D004820:Epidermolysis Bullosa / Q000382:microbiology*; D006801:Humans; D010802:Phylogeny; D013211:Staphylococcus aureus / Q000302:isolation & purification* / Q000502:physiology; D014947:Wounds and Injuries / Q000382:microbiology*