title: Gustatory receptor neurons in Manduca sexta contain a TrpA1-dependent signaling pathway that integrates taste and temperature.
abstract: Temperature modulates the peripheral taste response of many animals, in part by activating transient receptor potential (Trp) cation channels. We hypothesized that temperature would also modulate peripheral taste responses in larval Manduca sexta. We recorded excitatory responses of the lateral and medial styloconic sensilla to chemical stimuli at 14, 22, and 30 °C. The excitatory responses to 5 chemical stimuli-a salt (KCl), 3 sugars (sucrose, glucose, and inositol) and an alkaloid (caffeine)-were unaffected by temperature. In contrast, the excitatory response to the aversive compound, aristolochic acid (AA), increased robustly with temperature. Next, we asked whether TrpA1 mediates the thermally dependent taste response to AA. To this end, we 1) identified a TrpA1 gene in M. sexta; 2) demonstrated expression of TrpA1 in the lateral and medial styloconic sensilla; 3) determined that 2 TrpA1 antagonists (HC-030031 and mecamylamine) inhibit the taste response to AA, but not caffeine; and then 4) established that the thermal dependence of the taste response to AA is blocked by HC-030031. Taken together, our results indicate that TrpA1 serves as a molecular integrator of taste and temperature in M. sexta.
authors.affiliation: Department of Biology, Barnard College, Columbia University, 3009 Broadway, New York, NY 10027, USA. jglendin@barnard.edu
authors.forename: Anika
authors.initials: A
authors.lastname: Afroz
authors.forename: Natalie
authors.initials: N
authors.lastname: Howlett
authors.forename: Aditi
authors.initials: A
authors.lastname: Shukla
authors.forename: Farah
authors.initials: F
authors.lastname: Ahmad
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Batista
authors.forename: Katie
authors.initials: K
authors.lastname: Bedard
authors.forename: Sara
authors.initials: S
authors.lastname: Payne
authors.forename: Brian
authors.initials: B
authors.lastname: Morton
authors.forename: Jennifer H
authors.initials: JH
authors.lastname: Mansfield
authors.forename: John I
authors.initials: JI
authors.lastname: Glendinning
mesh_terms: D000083:Acetanilides; D000818:Animals; D034341:Aristolochic Acids / Q000494:pharmacology; D002110:Caffeine / Q000494:pharmacology; D019476:Insect Proteins / Q000235:genetics / Q000378:metabolism*; D018402:Manduca / Q000235:genetics / Q000502:physiology*; D008437:Maxilla / Q000502:physiology; D009474:Neurons / Q000378:metabolism*; D011687:Purines; D011956:Receptors, Cell Surface / Q000378:metabolism; D058618:Sensilla / Q000502:physiology; D015398:Signal Transduction / Q000502:physiology; D050052:TRPC Cation Channels / Q000037:antagonists & inhibitors / Q000378:metabolism; D013649:Taste / Q000187:drug effects / Q000502:physiology*; D013696:Temperature
chemical_list: C552888:2-(1,3-dimethyl-2,6-dioxo-1,2,3,6-tetrahydro-7H-purin-7-yl)-N-(4-isopropylphenyl)acetamide; D000083:Acetanilides; D034341:Aristolochic Acids; D019476:Insect Proteins; D011687:Purines; D011956:Receptors, Cell Surface; D050052:TRPC Cation Channels; D002110:Caffeine; C000228:aristolochic acid I
keywords: TrpA1; aristolochic acid; insect; taste; temperature