title: Effect of cytokinins on in vitro multiplication of Sophora tonkinensis.
abstract: OBJECTIVE
  To determine the effects of different cytokinins at various concentrations on in vitro shoot multiplication of an important medicinal plant.
  
  
  METHODS
  Nodal explants (1.5-2.0 cm) of Sophora tonkinensis were used. Multiple shoots were induced from nodal explants cultured on the Murashige and Skoog (MS) medium supplemented with 0.0, 0.5, 1.0, 2.0, 4.0, 8.0, or 16.0 µmol 2-isopentyladenine (2iP), N6 benzyladenine, kinetin or thiadiazuron.
  
  
  RESULTS
  Among the four investigated cytokinins, 2iP showed the best response for shoot multiplication. Maximum shoot induction (75%) was achieved on the MS medium supplemented with 2.0 µmol 2iP, with a mean number of 5.0 shoots per explant. In comparison to other cytokinins tried, 2iP showed the highest shoot elongation with a mean shoot length of 4.8 cm. Root initiation was observed within 15 d within the transfer of shoots onto the MS basal medium, and the rooting percentage was 100% with a mean number of 5.4 roots per shoot and root length of 6.2 cm over a period of 4 weeks. The healthy plants, hardened and transferred to a greenhouse for proper acclimatization, exhibited 100% survival.
  
  
  CONCLUSIONS
  It can be summarized that 2iP is the optimal plant growth regulator for Sophora multiplication.
authors.affiliation: Research Institute of Life Science, Gyeongsang National University, Jinju 660-701, South Korea.
authors.forename: Sonali
authors.initials: S
authors.lastname: Jana
authors.forename: Iyyakkannu
authors.initials: I
authors.lastname: Sivanesan
authors.forename: Byoung Ryong
authors.initials: BR
authors.lastname: Jeong
authors.forename: Krishna Kumar
authors.initials: KK
authors.lastname: Sugumaran
mesh_terms: D000064:Acclimatization; D003470:Culture Media / Q000494:pharmacology; D003583:Cytokinins / Q000494:pharmacology*; D066298:In Vitro Techniques; D018519:Meristem / Q000187:drug effects* / Q000254:growth & development*; D010937:Plant Growth Regulators / Q000494:pharmacology*; D010946:Plants, Medicinal; D012038:Regeneration; D029909:Sophora
chemical_list: D003470:Culture Media; D003583:Cytokinins; D010937:Plant Growth Regulators
keywords: 2-isopentyladenine; Herbaceous legumes; Kinetin; Nodal explants; Shoot regeneration