title: Silica-based cerium (III) chloride nanoparticles prevent the fructose-induced glycation of α-crystallin and H₂O₂-induced oxidative stress in human lens epithelial cells.
abstract: This study aimed to investigate whether silica-cerium (III) chloride (CeCl3) nanoparticles could inhibit the formation of advanced glycation end-products (AGEs) and reduce oxidative stress. Silica-CeCl3 nanoparticles were synthesised by adsorption and embedment with micro-silica materials, forming uniform nanoparticles with a diameter of approximately 130 nm. Chaperone activity assays and AGEs formation assays, and intracellular reactive assays were adopted in this study to evaluate CeCl3 nanoparticles effect. UV-visible spectrometry showed that silica-CeCl3 nanoparticles at low concentrations rapidly formed tentatively stable conjugations with α-crystallin, greatly enhancing the chaperone activity of α-crystallin. Moreover, silica-CeCl3 nanoparticles markedly inhibited the fructose-induced glycation of α-crystallin, showing an advantage over the control drugs aminoguanidine and carnosine. Silica-CeCl3 nanoparticles also reduced intracellular reactive oxygen species production and restored glutathione levels in H2O2-treated human lens epithelial cells. These findings suggest that silica-CeCl3 may be used as a novel agent for the prevention of cataractogenesis.
authors.affiliation: Department of Ophthalmology, Eye and ENT Hospital, Fudan University, 83 Fenyang Road, Shanghai, 200031, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Yang
authors.forename: Lei
authors.initials: L
authors.lastname: Cai
authors.forename: Sen
authors.initials: S
authors.lastname: Zhang
authors.forename: Xiangjia
authors.initials: X
authors.lastname: Zhu
authors.forename: Peng
authors.initials: P
authors.lastname: Zhou
authors.forename: Yi
authors.initials: Y
authors.lastname: Lu
mesh_terms: D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D002563:Cerium / Q000494:pharmacology*; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism; D005632:Fructose / Q000633:toxicity*; D006031:Glycosylation / Q000187:drug effects; D006801:Humans; D006861:Hydrogen Peroxide / Q000633:toxicity*; D007908:Lens, Crystalline / Q000187:drug effects* / Q000378:metabolism; D053768:Metal Nanoparticles; D018384:Oxidative Stress / Q000187:drug effects* / Q000502:physiology; D017382:Reactive Oxygen Species / Q000378:metabolism; D012822:Silicon Dioxide / Q000494:pharmacology; D038201:alpha-Crystallins / Q000378:metabolism
chemical_list: D017382:Reactive Oxygen Species; D038201:alpha-Crystallins; D005632:Fructose; D002563:Cerium; D012822:Silicon Dioxide; D006861:Hydrogen Peroxide; C026690:cerous chloride