title: Optimization of polysaccharides from Lycium ruthenicum fruit using RSM and its anti-oxidant activity.
abstract: Dynamic microwave-assisted extraction (DMAE) technique was employed for the extraction of polysaccharides from Lycium ruthenicum (LRP). The extracting parameters were optimized by using three-variable-three-level Box-Behnken design and response surface methodology (RSM) based on the single-factor experiments. RSM analysis indicated good correspondence between experimental and predicted values. The optimum extraction parameters for the yield of polysaccharide were ratio of water to raw material 31.5 mL/g, extracting time 25.8 min and microwave power 544.0 W. Polysaccharide was analyzed by chemical methods and Fourier-transform infrared (FT-IR). The antioxidant activities of LRP were investigated including scavenging activity of 2,2-diphenyl-1-picrylhydrazyl (DPPH), hydrogen peroxide and free radicals of superoxide anion in vitro. The results of antioxidant activity exhibited LRP had the potential to be explored as novel natural antioxidant for using in functional foods or medicine.
authors.affiliation: Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810001, PR China; University of Chinese Academy of Sciences, Beijing 100049, PR China.
authors.forename: Zenggen
authors.initials: Z
authors.lastname: Liu
authors.forename: Jun
authors.initials: J
authors.lastname: Dang
authors.forename: Qilan
authors.initials: Q
authors.lastname: Wang
authors.forename: Minfeng
authors.initials: M
authors.lastname: Yu
authors.forename: Lei
authors.initials: L
authors.lastname: Jiang
authors.forename: Lijuan
authors.initials: L
authors.lastname: Mei
authors.forename: Yun
authors.initials: Y
authors.lastname: Shao
authors.forename: Yanduo
authors.initials: Y
authors.lastname: Tao
mesh_terms: D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D005638:Fruit / Q000737:chemistry*; D017665:Hydroxyl Radical / Q000037:antagonists & inhibitors; D032305:Lycium / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D000975:Antioxidants; D010936:Plant Extracts; D011134:Polysaccharides; D017665:Hydroxyl Radical
keywords: Antioxidant activity; Lycium ruthenicum Murr.; Polysaccharides