title: IL4 and IFNalpha generation of dendritic cells reveals great migratory potential and NFkB and cJun expression in IL4DCs.
abstract: Dendritic cells (DCs) recently revealed as a potent tumor vaccine component, are commonly differentiated from monocytes by cultivation with IL-4 and GM-CSF. Despite the different opinions, the use of IFNalpha can promote DCs differentiation and activation. The aim of this study was to compare the functionality and phenotypic characterization of monocyte-derived DC generated by IL-4 (IL4DC) and IFNalpha (IFNalphaDC) modified protocols. To this aim, we investigated the expression of maturation markers, co-stimulatory molecules, relevant miRNA, cytokine and migratory profiles and the functional ability of these cells to stimulate autologous T cells in vitro. We herein investigated the molecular mechanism underlying the parameters previously described, as the relative expression of NF-kB p65, c-fos and c-jun, transcription factors. Our results demonstrated that IL4DC presented a stable phenotype, an increase in migratory capacity and NF-KB activation, in addition to lower levels of miR-146 a and miR-221. We believe that the IL4DC migratory potential and increase in NFkBp65 expression may be involved in higher IL12 expression and migration, suggesting a preferential activation of TH1 immune responses by IL4DC.
authors.affiliation: Hemocentro, University of Campinas , Campinas, SP , Brazil.
authors.forename: Maria Teresa Almeida
authors.initials: MT
authors.lastname: de Azevedo
authors.forename: Sara Teresinha Olalla
authors.initials: ST
authors.lastname: Saad
authors.forename: Simone Cristina Olenscki
authors.initials: SC
authors.lastname: Gilli
mesh_terms: D000943:Antigens, Differentiation / Q000378:metabolism; D019496:Cancer Vaccines; D002454:Cell Differentiation; D019070:Cell Lineage; D002465:Cell Movement; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000276:immunology* / Q000637:transplantation; D006801:Humans; D016130:Immunophenotyping; D016898:Interferon-alpha / Q000276:immunology*; D018664:Interleukin-12 / Q000235:genetics / Q000378:metabolism; D015847:Interleukin-4 / Q000276:immunology*; D048031:JNK Mitogen-Activated Protein Kinases / Q000378:metabolism; D008213:Lymphocyte Activation; D035683:MicroRNAs / Q000378:metabolism; D016328:NF-kappa B / Q000378:metabolism; D018417:Th1 Cells / Q000276:immunology*; D058408:Th1-Th2 Balance
chemical_list: D000943:Antigens, Differentiation; D019496:Cancer Vaccines; D016898:Interferon-alpha; D035683:MicroRNAs; D016328:NF-kappa B; D018664:Interleukin-12; D015847:Interleukin-4; D048031:JNK Mitogen-Activated Protein Kinases