title: Simultaneous analysis of Nε-(carboxymethyl)lysine, reducing sugars, and lysine during the dairy thermal process.
abstract: A new analytical method allowing the simultaneous quantification of Nε-(carboxymethyl)lysine (CML), lysine, and reducing sugars (glucose, lactose, and galactose) is described. It is based on high performance anion-exchange chromatography with pulsed amperometric electrochemical detection. This method demonstrated a low limit of quantification (0.385 to 0.866 mg/L), excellent linear correlation (R(2)>0.997), and desired calibration range (3.125 to 25 mg/L). In addition, lactose-lysine solutions containing sulfite (4 to 400 mmol/L) were heated at 110°C for 2h. The results showed that sulfite inhibited the formation of CML and promoted the consumption of reducing sugars and lysine in the Maillard reaction model. The method proved to be useful for simultaneous analysis of CML, lysine, and reducing sugars (glucose, galactose, and lactose) in the Maillard reaction system. Moreover, sulfite was an effective inhibitor of CML formation.
authors.affiliation: College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, China.
authors.forename: Xian-Bing
authors.initials: XB
authors.lastname: Xu
authors.forename: Fei
authors.initials: F
authors.lastname: Ma
authors.forename: Shu-Juan
authors.initials: SJ
authors.lastname: Yu
authors.forename: Yong-Guang
authors.initials: YG
authors.lastname: Guan
mesh_terms: D000818:Animals; D002852:Chromatography, Ion Exchange / Q000379:methods; D005690:Galactose / Q000032:analysis*; D005947:Glucose / Q000032:analysis*; D007785:Lactose / Q000032:analysis*; D008239:Lysine / Q000031:analogs & derivatives* / Q000032:analysis*; D015416:Maillard Reaction; D008892:Milk / Q000737:chemistry*
chemical_list: C048496:N(6)-carboxymethyllysine; D005947:Glucose; D007785:Lactose; D008239:Lysine; D005690:Galactose
keywords: Maillard reaction; Nε-(carboxymethyl)lysine; advanced glycation end product; high performance anion-exchange chromatography with pulsed amperometric electrochemical detection (HPAEC-PAD)