title: Molecularly imprinted polymer in microextraction by packed sorbent for the simultaneous determination of local anesthetics: lidocaine, ropivacaine, mepivacaine and bupivacaine in plasma and urine samples.
abstract: This study presents the use of molecularly imprinted polymer (MIP) as packing material for microextraction by packed syringe (MEPS) to achieve higher extraction selectivity. Pentycaine was used as template for MIP. Development and validation of the determination of lidocaine, ropivacaine, mepivacaine and bupivacaine in human plasma and urine samples utilizing MIP-MEPS and liquid chromatography-tandem mass spectrometry (LC-MS/MS) were carried out. The MEPS MIP-cartridge could be used for 100 extractions before it was discarded. The extraction recovery ranged from 60 to 80%. The correlation coefficients values were >0.999 for all assays using lidocaine, ropivacaine, mepivacaine and bupivacaine in the calibration range 5-2000 nmol/L. The accuracy of the studied compounds, given as a percentage variation from the nominal concentration values, ranged from -4.9 to 8.4% using plasma and urine samples. The between-batch precision, given as the relative standard deviation, at three different concentrations (quality control samples) was ranged from -4.7 to 14.0% and from 1.8 to 12.7% in plasma and urine, respectively. The lower limit of quantification and limit of detection of the studied substances were 5.0 and 1.0 nm, respectively.
authors.affiliation: Department of Analytical Chemistry, Stockholm University, SE-10691, Stockholm, Sweden; Faculty of Chemistry, Bu Ali-Sina University, Hamedan, Iran.
authors.forename: Seyed Mosayeb
authors.initials: SM
authors.lastname: Daryanavard
authors.forename: Amin
authors.initials: A
authors.lastname: Jeppsson-Dadoun
authors.forename: Lars I
authors.initials: LI
authors.lastname: Andersson
authors.forename: Mahdi
authors.initials: M
authors.lastname: Hashemi
authors.forename: Anders
authors.initials: A
authors.lastname: Colmsjö
authors.forename: Mohamed
authors.initials: M
authors.lastname: Abdel-Rehim
mesh_terms: D000577:Amides / Q000097:blood / Q000302:isolation & purification / Q000652:urine; D000779:Anesthetics, Local / Q000097:blood* / Q000302:isolation & purification / Q000652:urine*; D002045:Bupivacaine / Q000097:blood / Q000302:isolation & purification / Q000652:urine; D002853:Chromatography, Liquid / Q000379:methods*; D006801:Humans; D008012:Lidocaine / Q000097:blood / Q000302:isolation & purification / Q000652:urine; D057230:Limit of Detection; D008619:Mepivacaine / Q000097:blood / Q000302:isolation & purification / Q000652:urine; D054802:Molecular Imprinting; D011108:Polymers / Q000737:chemistry; D000077212:Ropivacaine; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D000577:Amides; D000779:Anesthetics, Local; D011108:Polymers; D000077212:Ropivacaine; D008012:Lidocaine; D008619:Mepivacaine; D002045:Bupivacaine
keywords: LC-MS/MS; MEPS; MIP; local anesthetic drugs; plasma and urine samples