title: A strategy for enhancing the engraftment of human hematopoietic stem cells in NOD/SCID mice.
abstract: To overcome the limitations of allogeneic hematopoietic stem cell transplantation (HSCT), we conducted a study to identify a strategy for enhancing hematopoietic stem cell (HSC) engraftment during HSCT. Co-transplantation experiments with mesenchymal stem cells (MSCs) derived from adult human tissues including bone marrow (BM), adipose tissue (AT), and umbilical cord blood (CB) were conducted. We showed that AT-MSCs and CB-MSCs enhanced the engraftment of HSCs as effectively as BM-MSCs in NOD/SCID mice, suggesting that AT-MSCs and CB-MSCs can be used as alternative stem cell sources for enhancing the engraftment and homing of HSCs. CB-MSCs derived from different donors showed different degrees of efficacy in enhancing the engraftment of HSCs. The most effective CB-MSCs showed higher proliferation rates and secreted more MCP-1, RANTES, EGF, and VEGF. Our results suggest that AT-MSCs and CB-MSCs could be alternative stem cell sources for co-transplantation in HSCT. Furthermore, in terms of MSCs' heterogeneity, characteristics of each population of MSCs are considerable factors for selecting MSCs suitable for co-transplantation with HSC.
authors.affiliation: Department of Pediatrics, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, South Korea.
authors.forename: Soo Hyun
authors.initials: SH
authors.lastname: Lee
authors.forename: Dae Seong
authors.initials: DS
authors.lastname: Kim
authors.forename: Myoung Woo
authors.initials: MW
authors.lastname: Lee
authors.forename: Yoo Hun
authors.initials: YH
authors.lastname: Noh
authors.forename: In Keun
authors.initials: IK
authors.lastname: Jang
authors.forename: Dong Hyun
authors.initials: DH
authors.lastname: Kim
authors.forename: Heung-Mo
authors.initials: HM
authors.lastname: Yang
authors.forename: Sung-Joo
authors.initials: SJ
authors.lastname: Kim
authors.forename: Soo Jin
authors.initials: SJ
authors.lastname: Choi
authors.forename: Wonil
authors.initials: W
authors.lastname: Oh
authors.forename: Yoon Sun
authors.initials: YS
authors.lastname: Yang
authors.forename: Hee Won
authors.initials: HW
authors.lastname: Chueh
authors.forename: Meong Hi
authors.initials: MH
authors.lastname: Son
authors.forename: Hye Lim
authors.initials: HL
authors.lastname: Jung
authors.forename: Keon Hee
authors.initials: KH
authors.lastname: Yoo
authors.forename: Ki Woong
authors.initials: KW
authors.lastname: Sung
authors.forename: Hong Hoe
authors.initials: HH
authors.lastname: Koo
mesh_terms: D000273:Adipose Tissue / Q000166:cytology / Q000502:physiology / Q000637:transplantation; D000818:Animals; D001854:Bone Marrow Cells / Q000502:physiology; D049109:Cell Proliferation; D002478:Cells, Cultured; D005312:Fetal Blood / Q000502:physiology / Q000637:transplantation; D006085:Graft Survival / Q000502:physiology*; D018380:Hematopoietic Stem Cell Transplantation / Q000379:methods*; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000502:physiology*; D051379:Mice; D016688:Mice, Inbred NOD; D016513:Mice, SCID