title: Assessing β-amyloid-induced NLRP3 inflammasome activation in primary microglia.
abstract: Senile plaques are an important histological hallmark of Alzheimer's disease. They mainly consist of the fibrillar peptide β-amyloid (Aβ) and are surrounded by activated microglia and astrocytes. Microglia in the vicinity of senile plaques express high levels of proinflammatory cytokines and neurotoxic substances, which are believed to influence disease progression. One important cytokine in Alzheimer's disease is IL-1β. Stimulation of cultured primary microglia by synthetic fibrillar Aβ causes the release of IL-1β via activation of the NLRP3 inflammasome.Here we provide protocols for the preparation of primary microglial cultures and synthetic oligomeric and fibrillar forms of Aβ.
authors.affiliation: Max-Planck Research Group Neuroimmunology, Center of Advanced European Studies and Research (caesar), Bonn, Germany.
authors.forename: Mareike
authors.initials: M
authors.lastname: Schnaars
authors.forename: Hannes
authors.initials: H
authors.lastname: Beckert
authors.forename: Annett
authors.initials: A
authors.lastname: Halle
mesh_terms: D016229:Amyloid beta-Peptides / Q000302:isolation & purification / Q000494:pharmacology*; D000818:Animals; D002352:Carrier Proteins / Q000378:metabolism*; D002469:Cell Separation / Q000379:methods; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D058847:Inflammasomes / Q000378:metabolism*; D053583:Interleukin-1beta / Q000096:biosynthesis; D051379:Mice; D017628:Microglia / Q000187:drug effects* / Q000378:metabolism*; D000071199:NLR Family, Pyrin Domain-Containing 3 Protein; D061251:Primary Cell Culture / Q000379:methods
chemical_list: D016229:Amyloid beta-Peptides; D002352:Carrier Proteins; D058847:Inflammasomes; D053583:Interleukin-1beta; D000071199:NLR Family, Pyrin Domain-Containing 3 Protein; C492716:Nlrp3 protein, mouse