title: Dissemination of metallo-β-lactamase-producing Pseudomonas aeruginosa of sequence type 235 in Asian countries.
abstract: OBJECTIVE
  To investigate the epidemiological traits of metallo-β-lactamase (MBL)-producing Pseudomonas aeruginosa (MPPA) clinical isolates collected by the Asian Network for Surveillance of Resistant Pathogens (ANSORP).
  
  
  METHODS
  A total of 16 MPPA clinical isolates were collected from six Asian countries in 2000 to 2009 by ANSORP. The MBL gene was detected by PCR amplification. The genetic organization of the class 1 integron carrying the MBL gene cassette was investigated by PCR mapping and sequencing. Southern blotting, repetitive sequence-based PCR and multilocus sequence typing (MLST) experiments were performed to characterize the isolates.
  
  
  RESULTS
  PCR and sequencing experiments detected the blaVIM-2 (n = 12), blaVIM-3 (n = 1), blaIMP-6 (n = 2) and blaIMP-26 (n = 1) genes. The MBL genes were located on the chromosome in all isolates except one. Furthermore, all the MBL genes were located in a class 1 integron. All the MPPA isolates from Malaysia, Thailand, Sri Lanka and Korea were identified as sequence type (ST) 235 by MLST. Three VIM-2-producing isolates from India were identified as ST773, and one isolate harbouring VIM-3 from Taiwan was identified as ST298.
  
  
  CONCLUSIONS
  P. aeruginosa ST235 might play a role in dissemination of MBL genes in Asian countries.
authors.affiliation: Department of Biomedical Laboratory Science, Eulji University College of Health Science, Seongnam, Korea.
authors.forename: Moon Jung
authors.initials: MJ
authors.lastname: Kim
authors.forename: Il Kwon
authors.initials: IK
authors.lastname: Bae
authors.forename: Seok Hoon
authors.initials: SH
authors.lastname: Jeong
authors.forename: So Hyun
authors.initials: SH
authors.lastname: Kim
authors.forename: Jae Hoon
authors.initials: JH
authors.lastname: Song
authors.forename: Jae Young
authors.initials: JY
authors.lastname: Choi
authors.forename: Sang Sun
authors.initials: SS
authors.lastname: Yoon
authors.forename: Visanu
authors.initials: V
authors.lastname: Thamlikitkul
authors.forename: Po-Ren
authors.initials: PR
authors.lastname: Hsueh
authors.forename: Rohani Md
authors.initials: RM
authors.lastname: Yasin
authors.forename: M K
authors.initials: MK
authors.lastname: Lalitha
authors.forename: Kyungwon
authors.initials: K
authors.lastname: Lee
mesh_terms: D001208:Asia / Q000453:epidemiology; D015139:Blotting, Southern; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D005838:Genotype; D006801:Humans; D017720:Molecular Epidemiology; D058889:Molecular Typing; D016133:Polymerase Chain Reaction; D011552:Pseudomonas Infections / Q000453:epidemiology* / Q000382:microbiology* / Q000635:transmission; D011550:Pseudomonas aeruginosa / Q000145:classification* / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification; D017422:Sequence Analysis, DNA; D001618:beta-Lactamases / Q000235:genetics / Q000378:metabolism*
chemical_list: D004269:DNA, Bacterial; D001618:beta-Lactamases
keywords: IMP-26; IMP-6; VIM-2; VIM-3; class 1 integrons