title: Assembly and features of secondary metabolite biosynthetic gene clusters in Streptomyces ansochromogenes.
abstract: A draft genome sequence of Streptomyces ansochromogenes 7100 was generated using 454 sequencing technology. In combination with local BLAST searches and gap filling techniques, a comprehensive antiSMASH-based method was adopted to assemble the secondary metabolite biosynthetic gene clusters in the draft genome of S. ansochromogenes. A total of at least 35 putative gene clusters were identified and assembled. Transcriptional analysis showed that 20 of the 35 gene clusters were expressed in either or all of the three different media tested, whereas the other 15 gene clusters were silent in all three different media. This study provides a comprehensive method to identify and assemble secondary metabolite biosynthetic gene clusters in draft genomes of Streptomyces, and will significantly promote functional studies of these secondary metabolite biosynthetic gene clusters.
authors.affiliation: State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Xingyu
authors.initials: X
authors.lastname: Zhong
authors.forename: Yuqing
authors.initials: Y
authors.lastname: Tian
authors.forename: Guoqing
authors.initials: G
authors.lastname: Niu
authors.forename: Huarong
authors.initials: H
authors.lastname: Tan
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry; D003360:Cosmids; D005798:Genes, Bacterial; D016680:Genome, Bacterial; D005810:Multigene Family; D016366:Open Reading Frames; D020133:Reverse Transcriptase Polymerase Chain Reaction; D064210:Secondary Metabolism; D015398:Signal Transduction; D013302:Streptomyces / Q000235:genetics* / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: D000900:Anti-Bacterial Agents