title: Polymorphic variation of CYP1A1 and CYP1B1 genes in a Haryana population.
abstract: Cytochrome P450 (CYP) 1A1 and CYP1B1 are important phase I xenobiotic metabolizing enzymes involved in the metabolism of numbers of toxins, endogenous hormones, and pharmaceutical drugs. Polymorphisms in these phase I genes can alter enzyme activity and are known to be associated with cancer susceptibility related to environmental toxins and hormone exposure. Their genotypes may also display ethnicity-dependent population frequencies. The present study was aimed to determine the frequencies of commonly known functional polymorphisms of CYP1A1 and CYP1B1 genes in a Haryana state population of North India. The allelic frequency of CYP1A1 polymorphism m1 (MspI) was 29.65% and m2 (Ile(462)Val) was 24.85%. The frequency of CYP1B1 polymorphism m1 (Val(432)Leu) was 45.85% and m2 (Asn(453)Ser) was 16.2%. We observed inter- and intra-ethnic variation in the frequency distribution of these polymorphisms. Analysis of polymorphisms in these genes might help in predicting the risk of cancer. Our results emphasize the need for more such studies in high-risk populations.
authors.affiliation: Department of Biotechnology, Kurukshetra University, Kurukshetra, 136119, Haryana, India.
authors.forename: Shiv Kumar
authors.initials: SK
authors.lastname: Giri
authors.forename: Anita
authors.initials: A
authors.lastname: Yadav
authors.forename: Anil
authors.initials: A
authors.lastname: Kumar
authors.forename: Kapil
authors.initials: K
authors.lastname: Dev
authors.forename: Sachin
authors.initials: S
authors.lastname: Gulati
authors.forename: Ranjan
authors.initials: R
authors.lastname: Gupta
authors.forename: Neeraj
authors.initials: N
authors.lastname: Aggarwal
authors.forename: Sanjeev Kumar
authors.initials: SK
authors.lastname: Gautam
mesh_terms: D000328:Adult; D001189:Aryl Hydrocarbon Hydroxylases / Q000235:genetics*; D019363:Cytochrome P-450 CYP1A1 / Q000235:genetics*; D065633:Cytochrome P-450 CYP1B1; D005006:Ethnicity / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D007194:India; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012307:Risk Factors; D015262:Xenobiotics / Q000378:metabolism / Q000494:pharmacology; D055815:Young Adult
chemical_list: D015262:Xenobiotics; D001189:Aryl Hydrocarbon Hydroxylases; C569463:CYP1A1 protein, human; C585496:CYP1B1 protein, human; D019363:Cytochrome P-450 CYP1A1; D065633:Cytochrome P-450 CYP1B1