title: Protein-binding aptamer assisted signal amplification for the detection of influenza A (H1N1) DNA sequences based on quantum dot fluorescence polarization analysis.
abstract: We report a fluorescence polarization platform for H1N1 detection based on the construction of a DNA functional QD fluorescence polarization probe and a bi-functional protein binding aptamer (Apt-DNA). The assay has a linear range from 10 nM to 100 nM with a detection limit of 3.45 nM and is selective over the mismatched bases.
authors.affiliation: Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources (Ministry of Education of China), College of Chemistry and Chemical Engineering of Guangxi Normal University, Guilin, 541004, China.
authors.forename: Juanni
authors.initials: J
authors.lastname: Zhang
authors.forename: Jianniao
authors.initials: J
authors.lastname: Tian
authors.forename: Yanlong
authors.initials: Y
authors.lastname: He
authors.forename: Sheng
authors.initials: S
authors.lastname: Chen
authors.forename: Yixuan
authors.initials: Y
authors.lastname: Jiang
authors.forename: Yanchun
authors.initials: Y
authors.lastname: Zhao
authors.forename: Shulin
authors.initials: S
authors.lastname: Zhao
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry* / Q000378:metabolism*; D004279:DNA, Viral / Q000032:analysis* / Q000235:genetics; D005454:Fluorescence Polarization; D005456:Fluorescent Dyes / Q000737:chemistry / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D053118:Influenza A Virus, H1N1 Subtype / Q000235:genetics* / Q000302:isolation & purification; D009693:Nucleic Acid Hybridization; D045663:Quantum Dots; D019809:Streptavidin / Q000378:metabolism*
chemical_list: D052157:Aptamers, Nucleotide; D004279:DNA, Viral; D005456:Fluorescent Dyes; D019809:Streptavidin