title: A functional genomic analysis of Arabidopsis thaliana PP2C clade D.
abstract: In the reference dicot plant Arabidopsis thaliana, the PP2C family of P-protein phosphatases includes the products of 80 genes that have been separated into ten multi-protein clades plus six singletons. Clade D includes the products of nine genes distributed among three chromosomes (APD1, At3g12620; APD2, At3g17090; APD3, At3g51370; APD4, At3g55050; APD5, At4g33920; APD6, At4g38520; APD7, At5g02760; APD8, At5g06750; and APD9, At5g66080). As part of a functional genomics analysis of protein phosphorylation, we retrieved expression data from public databases and determined the subcellular protein localization of the members of clade D. While the nine proteins have been grouped together based upon primary sequence alignments, we observed no obvious common patterns in expression or localization. We found chimera with the GFP associated with the nucleus, plasma membrane, the endomembrane system, and mitochondria in transgenic plants.
authors.affiliation: Department of Biochemistry, University of Missouri, Columbia, MO, 65211, USA, Tovar-mendezA@missouri.edu.
authors.forename: Alejandro
authors.initials: A
authors.lastname: Tovar-Mendez
authors.forename: Ján A
authors.initials: JA
authors.lastname: Miernyk
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Hoyos
authors.forename: Douglas D
authors.initials: DD
authors.lastname: Randall
mesh_terms: D017360:Arabidopsis / Q000235:genetics*; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D018745:Genome, Plant; D023281:Genomics; D008856:Microscopy, Fluorescence
chemical_list: D029681:Arabidopsis Proteins