title: Modification of decellularized goat-lung scaffold with chitosan/nanohydroxyapatite composite for bone tissue engineering applications.
abstract: Decellularized goat-lung scaffold was fabricated by removing cells from cadaver goat-lung tissue, and the scaffold was modified with chitosan/nanohydroxyapatite composite for the purpose of bone tissue engineering applications. MTT assay with osteoblasts, seeded over the chitosan/nanohydroxyapatite-modified decellularized scaffold, demonstrated significantly higher cell growth as compared to the decellularized scaffold without modification. SEM analysis of cell-seeded scaffold, after incubation for 7 days, represented a good cell adhesion, and the cells spread over the chitosan/nanohydroxyapatite-modified decellularized scaffold. Expression of bone-tissue-specific osteocalcin gene in the osteoblast cells grown over the chitosan/nanohydroxyapatite-modified decellularized scaffold clearly signifies that the cells maintained their osteoblastic phenotype with the chitosan/nanohydroxyapatite-modified decellularized scaffold. Therefore, it can be concluded that the decellularized goat-lung scaffold-modified with chitosan/nanohydroxyapatite composite, may provide enhanced osteogenic potential when used as a scaffold for bone tissue engineering.
authors.affiliation: Department of Polymer and Process Engineering, Indian Institute of Technology Roorkee, Saharanpur Campus, Saharanpur, UP 247001, India.
authors.forename: Sweta K
authors.initials: SK
authors.lastname: Gupta
authors.forename: Amit K
authors.initials: AK
authors.lastname: Dinda
authors.forename: Pravin D
authors.initials: PD
authors.lastname: Potdar
authors.forename: Narayan C
authors.initials: NC
authors.lastname: Mishra
mesh_terms: D000818:Animals; D016025:Bone Transplantation; D001842:Bone and Bones; D002448:Cell Adhesion / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D048271:Chitosan / Q000008:administration & dosage / Q000737:chemistry*; D017886:Durapatite / Q000008:administration & dosage / Q000737:chemistry*; D006041:Goats / Q000254:growth & development / Q000502:physiology; D008168:Lung / Q000166:cytology; D053758:Nanoparticles / Q000008:administration & dosage / Q000737:chemistry; D010012:Osteogenesis / Q000187:drug effects; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry
chemical_list: D048271:Chitosan; D017886:Durapatite