title: Determination of median lethal dose of combination of endosulfan and cypermethrin in wistar rat.
abstract: The present study was designed to determine the lethal dose 50 (LD50) of combination of cypermethrin, a pyrethroid, and endosulfan, an organochlorine compound in Wistar rats. LD50 is the amount (dose) of a chemical, calculated as per the concentration of chemicals that produces death in 50% of a population of test animals to which it is administered by any of a variety of methods. A single oral dose of combination of cypermethrin and endosulfan were dissolved in dimethyl sulfoxide (DMSO) in a ratio of 1:1 and administered orally at the concentration of 165 mg/kg body weight (b.w), 330 mg/kg b.w, 660 mg/kg b.w, and 1320 mg/kg b.w to experimental animals. LD50 was calculated according to the method described by Miller and Tainter (1994) and was observed as 691.83 mg/kg b.w for this combination. Single dose of test article at 165 mg/kg b.w did not reveal any toxic signs or behavioral alterations, hence considered as No observed Adverse Effect level (NOAEL).
authors.affiliation: Department of Forensic Medicine and Toxicology, All India Institute of Medical Sciences, New Delhi, India.
authors.forename: Jaya
authors.initials: J
authors.lastname: Raj
authors.forename: Mohineesh
authors.initials: M
authors.lastname: Chandra
authors.forename: Tirath D
authors.initials: TD
authors.lastname: Dogra
authors.forename: Monika
authors.initials: M
authors.lastname: Pahuja
authors.forename: Anupuma
authors.initials: A
authors.lastname: Raina
keywords: Cypermethrin; LD50; endosulfan; no observed adverse effect level; organochlorine; pyrethroid