title: Preparative-cum-quantitative mass-directed analysis of swainsonine and its in situ activity against Sf-21 cell line.
abstract: Swainsonine is a polyhydroxy indolizidine alkaloid with various research and potential therapeutic applications. In this work, swainsonine was partially purified (2.5-folds) with acetone-methanol solvent system from Metarhizium anisopliae fermentation broth. The partially purified broth was further subjected to mass-directed preparative-cum-quantitative analysis. Swainsonine was eluted as MS1 fraction [M + H](+) 174.36 ± 0.21 at 4.91 ± 0.04 min with calculated yield of 7.85 ± 1.59 μg mL(-1) corresponding to 3.74 × 10(5) counts. In situ antiproliferative activity of standard and purified swainsonine fractions was tested against Spodoptera frugiperda, Sf-21 cell line with IC50 values of 2.96 μM and 3.28 μM, respectively, at 36 h. This analytical procedure for purification and quantitative analysis of swainsonine may ensure its suitability for routine laboratory studies and research.
authors.affiliation: Department of Biotechnology, Indian Institute of Technology Guwahati, Guwahati, Assam, India.
authors.forename: Digar
authors.initials: D
authors.lastname: Singh
authors.forename: Gurvinder
authors.initials: G
authors.lastname: Kaur
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D049109:Cell Proliferation / Q000187:drug effects*; D002853:Chromatography, Liquid; D020128:Inhibitory Concentration 50; D057230:Limit of Detection; D013058:Mass Spectrometry; D052981:Metarhizium / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry; D015203:Reproducibility of Results; D061987:Sf9 Cells; D018411:Spodoptera; D017026:Swainsonine / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D010936:Plant Extracts; D017026:Swainsonine
keywords: entomopathogen; mass-directed purification; quantification; selected ion monitoring; swainsonine