title: Construction and evaluation of an exopolysaccharide-producing engineered bacterial strain by protoplast fusion for microbial enhanced oil recovery.
abstract: Enterobacter cloacae strain JD, which produces water-insoluble biopolymers at optimal temperature of 30°C, and a thermophilic Geobacillus strain were used to construct an engineered strain for exopolysaccharide production at high temperatures by protoplast fusion. The obtained fusant strain ZR3 produced exopolysaccharides at up to 45°C with optimal growth temperature at 35°C. The fusant produced exopolysaccharides of approximately 7.5 g/L or more at pH between 7.0 and 9.0. The feasibility of the enhancement of crude oil recovery with the fusant was tested in a sand-packed column at 40°C. The results demonstrated that bioaugmentation of the fusant was promising approach for MEOR. Mass growth of the fusant was confirmed in fermentor tests.
authors.affiliation: State Key Laboratory of Heavy Oil Processing, Faculty of Chemical Engineering, China University of Petroleum, Beijing 102249, PR China.
authors.forename: Shanshan
authors.initials: S
authors.lastname: Sun
authors.forename: Yijing
authors.initials: Y
authors.lastname: Luo
authors.forename: Siyuan
authors.initials: S
authors.lastname: Cao
authors.forename: Wenhong
authors.initials: W
authors.lastname: Li
authors.forename: Zhongzhi
authors.initials: Z
authors.lastname: Zhang
authors.forename: Lingxi
authors.initials: L
authors.lastname: Jiang
authors.forename: Hanping
authors.initials: H
authors.lastname: Dong
authors.forename: Li
authors.initials: L
authors.lastname: Yu
authors.forename: Wei-Min
authors.initials: WM
authors.lastname: Wu
mesh_terms: D001673:Biodegradation, Environmental; D019149:Bioreactors / Q000382:microbiology; D016972:Enterobacter cloacae / Q000254:growth & development / Q000378:metabolism*; D005818:Genetic Engineering; D056506:Geobacillus / Q000254:growth & development / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D008561:Membrane Fusion; D009821:Oils / Q000378:metabolism*; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis*; D011523:Protoplasts / Q000378:metabolism*; D012822:Silicon Dioxide; D014867:Water
chemical_list: D009821:Oils; D011135:Polysaccharides, Bacterial; D014867:Water; D012822:Silicon Dioxide
keywords: Crude oil; Fusant; Microbial enhanced oil recovery (MEOR); Protoplast fusion; Water-insoluble exopolysaccharide