title: Interaction of E2F7 transcription factor with E2F1 and C-terminal-binding protein (CtBP) provides a mechanism for E2F7-dependent transcription repression.
abstract: Previous work has identified distinct functions for E2F proteins during a cellular proliferative response including a role for E2F1-3 in the activation of transcription at G1/S and a role for E2F4-8 in repressing the same group of E2F1-3 target genes as cells progress through S phase. We now find that E2F7 and E2F8, which are induced by E2F1-3 at G1/S, can form a heterodimer with E2F1 through interactions involving the DNA-binding domains of the two proteins. In vitro DNA interaction assays demonstrate the formation of an E2F1-E2F7 complex, as well as an E2F7-E2F7 complex on adjacent E2F-binding sites. We also show that E2F7 recruits the co-repressor C-terminal-binding protein (CtBP) and that CtBP2 is essential for E2F7 to repress E2F1 transcription. Taken together, these findings suggest a mechanism for the repression of transcription by E2F7.
authors.affiliation: Duke Institute for Genome Sciences and Policy, Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, North Carolina 27708, USA.
authors.forename: Beiyu
authors.initials: B
authors.lastname: Liu
authors.forename: Igor
authors.initials: I
authors.lastname: Shats
authors.forename: Steven P
authors.initials: SP
authors.lastname: Angus
authors.forename: Michael L
authors.initials: ML
authors.lastname: Gatza
authors.forename: Joseph R
authors.initials: JR
authors.lastname: Nevins
mesh_terms: D000429:Alcohol Oxidoreductases / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D056970:Co-Repressor Proteins; D050687:E2F1 Transcription Factor / Q000235:genetics / Q000378:metabolism*; D050702:E2F7 Transcription Factor / Q000235:genetics / Q000378:metabolism*; D016193:G1 Phase / Q000502:physiology; D057809:HEK293 Cells; D006801:Humans; D046912:Multiprotein Complexes / Q000235:genetics / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D055503:Protein Multimerization / Q000502:physiology; D017434:Protein Structure, Tertiary; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D016196:S Phase / Q000502:physiology; D014158:Transcription, Genetic / Q000502:physiology*
chemical_list: D056970:Co-Repressor Proteins; D050687:E2F1 Transcription Factor; C493753:E2F1 protein, human; D050702:E2F7 Transcription Factor; C481054:E2F7 protein, human; D046912:Multiprotein Complexes; D009419:Nerve Tissue Proteins; D012097:Repressor Proteins; D000429:Alcohol Oxidoreductases; C421248:CTBP2 protein, human
keywords: Cell Cycle; DNA-binding Protein; E2F Transcription Factor; Transcription Factors; Transcription Repressor