title: Assessing ATP binding and hydrolysis by NLR proteins.
abstract: Nucleotide-binding and leucine rich repeat domain-containing proteins (NLRs) are central to the formation of many inflammasome complexes. Several inflammasome forming NLR proteins are known to be ATPases, but the nucleotide binding specificity of many remains to be characterized. The oligomerization of NLR proteins and assembly of inflammasomes require the ATP (or other nucleotide) binding activity of the NLR proteins. Quantitative and qualitative studies of the nucleotide binding properties of these proteins are useful tools in studying the regulation of inflammasome activity, and are outlined in this Chapter.
authors.affiliation: Division of Infectious Diseases, Department of Medicine, University of North Carolina School of Medicine, Chapel Hill, NC, USA.
authors.forename: Jinyao
authors.initials: J
authors.lastname: Mo
authors.forename: Joseph A
authors.initials: JA
authors.lastname: Duncan
mesh_terms: D000251:Adenosine Triphosphatases / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism*; D000818:Animals; D004789:Enzyme Activation; D006801:Humans; D006868:Hydrolysis; D036341:Intercellular Signaling Peptides and Proteins / Q000302:isolation & purification / Q000378:metabolism*; D011485:Protein Binding; D013194:Staining and Labeling / Q000379:methods
chemical_list: D036341:Intercellular Signaling Peptides and Proteins; D000255:Adenosine Triphosphate; D000251:Adenosine Triphosphatases