title: Heat shock factor 1 inducers from the bark of Eucommia ulmoides as cytoprotective agents.
abstract: The barks of Eucommia ulmoides (Eucommiae Cortex, Eucommiaceae) have been used as a traditional medicine in Korea, Japan, and China to treat hypertension, reinforce the muscles and bones, and recover the damaged liver and kidney functions. Among these traditional uses, to establish the recovery effects on the damaged organs on the basis of phytochemistry, the barks of E. ulmoides have been investigated to afford three known phenolic compounds, coniferaldehyde glucoside (1), bartsioside (2), and feretoside (3), which were found in the family Eucommiaceae for the first time. The compounds 1-3 were evaluated for their inducible activities on the heat shock factor 1 (HSF1), and heat shock proteins (HSPs) 27 and 70, along with four compounds, geniposide (4), geniposidic acid (5), pinoresinol diglucoside (6), and liriodendrin (7), which were previously reported from E. ulmoides. Compounds 1-7 increased expression of HSF1 by a factor of 1.214, 1.144, 1.153, 1.114, 1.159, 1.041, and 1.167 at 3 μM, respectively. Coniferaldehyde glucoside (1) showed the most effective increase of HSF1 and induced successive expressions of HSP27 and HSP70 in a dose-dependent manner without cellular cytotoxicity, suggesting a possible application as a HSP inducer to act as cytoprotective agent.
authors.affiliation: The Center for Cell Signaling & Drug Discovery Research, College of Pharmacy, Ewha Womans University, Seoul, Korea.
authors.forename: Joo-Won
authors.initials: JW
authors.lastname: Nam
authors.forename: Seo-Young
authors.initials: SY
authors.lastname: Kim
authors.forename: Taesook
authors.initials: T
authors.lastname: Yoon
authors.forename: Yoo Jin
authors.initials: YJ
authors.lastname: Lee
authors.forename: Yun-Seo
authors.initials: YS
authors.lastname: Kil
authors.forename: Yun-Sil
authors.initials: YS
authors.lastname: Lee
authors.forename: Eun-Kyoung
authors.initials: EK
authors.lastname: Seo
mesh_terms: D002460:Cell Line; D004268:DNA-Binding Proteins / Q000378:metabolism*; D031283:Eucommiaceae / Q000737:chemistry*; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D055551:HSP27 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism; D000076249:Heat Shock Transcription Factors; D006801:Humans; D008518:Medicine, East Asian Traditional; D024301:Plant Bark / Q000737:chemistry; D011401:Promoter Regions, Genetic; D020011:Protective Agents / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D014157:Transcription Factors / Q000378:metabolism*
chemical_list: D004268:DNA-Binding Proteins; C000619706:HSF1 protein, human; D055551:HSP27 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins; D000076249:Heat Shock Transcription Factors; D020011:Protective Agents; D014157:Transcription Factors
keywords: Coniferaldehyde; Cytoprotective activity; Eucommia ulmoides; Glucosides; Heat shock factor