title: Transcriptome analysis reveals novel genes potentially involved in photoperiodic tuberization in potato.
abstract: Potato microtuber produced in vitro provides a model system to investigate photoperiod-dependent tuberization. However, the genes associated with potato tuberization remain to be elucidated. The present research involved three potato clones with distinct tuberization response to changes of photoperiod. Digital Gene Expression (DGE) Tag Profiling analysis of the short-day-sensitive clone identified 2218 genes that were regulated by day length. Both GO and KEGG pathway analysis provided insights into predominant biological processes and pathways, and enabled the selection of 56 genes associated with circadian rhythmicity, signal transduction, and development. Quantitative transcriptional analysis in the selected clones revealed 5 genes potentially associated with photoperiodic tuberization, which were predicted to encode a DOF protein, a blue light receptor, a lectin, a syntaxin-like protein, and a protein with unknown function. Our results strongly suggest that potato tuberization may be largely controlled by the homologs of genes shown to regulate flowering time in other plants.
authors.affiliation: National Center for Vegetable Improvement (Central China), People's Republic of China; Key Laboratory of Horticultural Plant Biology (Huazhong Agricultural University), Ministry of Education, People's Republic of China; Potato Engineering and Technology Research Center of Hubei Province, People's Republic of China; Huazhong Agricultural University, Wuhan, Hubei 430070, People's Republic of China. Electronic address: chanyujianwei@126.com.
authors.forename: Jianwei
authors.initials: J
authors.lastname: Shan
authors.forename: Wei
authors.initials: W
authors.lastname: Song
authors.forename: Jun
authors.initials: J
authors.lastname: Zhou
authors.forename: Xiaohuan
authors.initials: X
authors.lastname: Wang
authors.forename: Conghua
authors.initials: C
authors.lastname: Xie
authors.forename: Xiaoxi
authors.initials: X
authors.lastname: Gao
authors.forename: Tingting
authors.initials: T
authors.lastname: Xie
authors.forename: Jun
authors.initials: J
authors.lastname: Liu
mesh_terms: D019295:Computational Biology; D035264:Flowers / Q000235:genetics / Q000254:growth & development / Q000502:physiology; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D005838:Genotype; D058977:Molecular Sequence Annotation; D010641:Phenotype; D017440:Photoperiod; D035281:Plant Tubers / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*; D015398:Signal Transduction; D011198:Solanum tuberosum / Q000235:genetics / Q000254:growth & development*
keywords: CDF; CYCLING DOF FACTOR; DEG; DGE; DNA binding with one finger; DOF; FMN; JAME; LKP2; LOV; LOV KELCH REPEAT PROTEIN 2; Photoperiod; SNARE; Solanum tuberosum; TA; Transcriptome; Tuber; differentially expressed gene; digital gene expression; flavin mononucleotide; jasmonic acid methyl ester; light-oxygen-voltage; soluble N-ethylmaleimide-sensitive factor attachment protein receptors; tuberonic acid