title: Anti-edema effect of epigallocatechin gallate on spinal cord injury in rats.
abstract: Recent studies indicated that epigallocatechin gallate (EGCG) had neuroprotective effects on spinal cord injury (SCI).The current study was performed to determine the anti-edema effect of EGCG after SCI in rats. EGCG (100 mg/kg, i.p.) was administered to rats immediately following SCI. It was found that EGCG (100 mg/kg) could significantly reduce spinal cord water content. In addition, EGCG (100mg/kg) significantly reduced the expression of aquaporin-4(AQP4) and glial fibrillary acidic protein (GFAP) level at 24, 48 and 72h after injury, but it did not have this effect at 12 h after injury. The changes of AQP4 and GFAP protein induced by EGCG (100 mg/kg) treatment were accompanied by a reduction of spinal cord edema. Our results indicated that EGCG (100 mg/kg) could reduce spinal cord edema after SCI, which could be correlated with the down-regulation the expression of AQP4 and GFAP protein level after SCI.
authors.affiliation: Department of Orthopaedics, The First Affiliated Hospital of China Medical University, No.155 Nanjing North Street, Heping District, Shenyang 110001, Liaoning Province, PR China.
authors.forename: Rui
authors.initials: R
authors.lastname: Ge
authors.forename: Yue
authors.initials: Y
authors.lastname: Zhu
authors.forename: Yao
authors.initials: Y
authors.lastname: Diao
authors.forename: Lin
authors.initials: L
authors.lastname: Tao
authors.forename: Wei
authors.initials: W
authors.lastname: Yuan
authors.forename: Xiao-chuan
authors.initials: XC
authors.lastname: Xiong
mesh_terms: D000818:Animals; D051401:Aquaporin 4 / Q000096:biosynthesis / Q000187:drug effects; D015153:Blotting, Western; D002392:Catechin / Q000031:analogs & derivatives* / Q000494:pharmacology; D004195:Disease Models, Animal; D004487:Edema / Q000378:metabolism / Q000517:prevention & control*; D005904:Glial Fibrillary Acidic Protein / Q000096:biosynthesis / Q000187:drug effects; D007150:Immunohistochemistry; D008297:Male; D018696:Neuroprotective Agents / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013119:Spinal Cord Injuries / Q000378:metabolism* / Q000473:pathology
chemical_list: C496032:Aqp4 protein, rat; D051401:Aquaporin 4; D005904:Glial Fibrillary Acidic Protein; D018696:Neuroprotective Agents; D002392:Catechin; C045651:epigallocatechin gallate
keywords: Aquaporin-4; Edema; Epigallocatechin gallate; Glial fibrillary acidic protein; Spinal cord injury