title: Molecular characterization of manganese peroxidases from white-rot fungus Polyporus brumalis.
abstract: The cDNAs of six manganese-dependent peroxidases (MnPs) were isolated from white-rot fungus Polyporus brumalis. The MnP proteins shared similar properties with each other in terms of size (approximately 360-365 amino acids) and primary structure, showing 62-96 % amino acid sequence identity. RT-PCR analysis indicated that these six genes were predominantly expressed in shallow stationary culture (SSC) in a liquid medium. Gene expression was induced by treatment with dibutyl phthalate (DBP) and wood chips. Expression of pbmnp4 was strongly induced by both treatments, whereas that of pbmnp5 was induced only by DBP, while pbmnp6 was induced by wood chips only. Then, we overexpressed pbmnp4 in P. brumalis under the control of the GPD promoter. Overexpression of pbmnp4 effectively increased MnP activity; the transformant that had the highest MnP activity also demonstrated the most effective decolorization of Remazol Brilliant Blue R dye. Identification of MnP cDNAs can contribute to the efficient production of lignin-degradation enzymes and may lead to utilization of basidiomycetous fungi for degradation of lignin and numerous recalcitrant xenobiotics.
authors.affiliation: Division of Wood Chemistry and Microbiology, Department of Forest Products, Korea Forest Research Institute, Seoul, 130-712, South Korea.
authors.forename: Sun-Hwa
authors.initials: SH
authors.lastname: Ryu
authors.forename: Boyeong
authors.initials: B
authors.lastname: Kim
authors.forename: Myungkil
authors.initials: M
authors.lastname: Kim
authors.forename: Jin-Ho
authors.initials: JH
authors.lastname: Seo
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D017931:DNA Primers; D018076:DNA, Complementary; D003993:Dibutyl Phthalate / Q000378:metabolism; D008969:Molecular Sequence Data; D010544:Peroxidases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D055492:Polyporus / Q000201:enzymology*; D011401:Promoter Regions, Genetic; D017386:Sequence Homology, Amino Acid
chemical_list: D017931:DNA Primers; D018076:DNA, Complementary; D003993:Dibutyl Phthalate; D010544:Peroxidases; C051129:manganese peroxidase