title: Detection and mechanism of action of ESM-1 in rat kidney transplantation under various immune states.
abstract: OBJECTIVE
  To investigate whether ESM-1 expression change reflects the impairment of endothelial cells and rejection after kidney transplantation, ESM-1 expression was detected under various immune states in this study.
  
  
  METHODS
  Kidney transplantations were performed from BN to LEW rats. Syngenic LEW-LEW grafts were used as controls. The LEW recipient rats were divided into acute rejection (AR) group, ciclosporin A (CsA) group and control group. In each group, 10 rats were sacrificed at 1, 5, and 7d after operation, respectively, and blood and kidney samples were collected. In the rat model of kidney transplantation, ESM-1 mRNA and ESM-1 protein expression were detected in various immune states to verify if ESM-1 can reflect endothelial cell impairment sensitively.
  
  
  RESULTS
  ESM-1 mRNA (1d vs. 3d, P<0.01;3d vs. 7d, P=0.018) and ESM-1 protein expression was upregulated significantly in the AR group (P<0.01, 5 and 7d), when compared to CsA group and control group. In CsA group, the cell apoptosis rate decreased when compared to AR group (P<0.01). Pathological impairment was more serious in AR group than in CsA group (P<0.01).
  
  
  CONCLUSIONS
  Peripheral blood ESM-1 mRNA and ESM-1 protein expression in kidney grafts can reflect the severity of endothelial cell impairment. Thus, ESM-1 may be used as a new indicator for AR prediction and diagnosis. Nevertheless, further investigation is required to test if it meets the criteria for clinical utility.
authors.affiliation: Department of Urology, The General Hospital of Chengdu Military Region, Chengdu, China. lishadan@hotmail.com
authors.forename: Shadan
authors.initials: S
authors.lastname: Li
authors.forename: Ping
authors.initials: P
authors.lastname: Liang
authors.forename: Youguang
authors.initials: Y
authors.lastname: Zhao
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Li
authors.forename: Yu
authors.initials: Y
authors.lastname: Hu
authors.forename: Wei
authors.initials: W
authors.lastname: Wu
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.forename: Peng
authors.initials: P
authors.lastname: Zhou
authors.forename: Qiwu
authors.initials: Q
authors.lastname: Wang
authors.forename: Wei
authors.initials: W
authors.lastname: Yang
authors.forename: Liang
authors.initials: L
authors.lastname: Wang
authors.forename: Qingtang
authors.initials: Q
authors.lastname: Wang
authors.forename: Hang
authors.initials: H
authors.lastname: Yang
authors.forename: Weiguo
authors.initials: W
authors.lastname: Cheng
authors.forename: Wenfeng
authors.initials: W
authors.lastname: Chao
authors.forename: Binghong
authors.initials: B
authors.lastname: Zhang
authors.forename: Fengshuo
authors.initials: F
authors.lastname: Jin
mesh_terms: D000818:Animals; D015153:Blotting, Western; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D006084:Graft Rejection / Q000276:immunology / Q000378:metabolism*; D016030:Kidney Transplantation / Q000502:physiology*; D011509:Proteoglycans / Q000378:metabolism*; D051381:Rats; D011917:Rats, Inbred Lew; D060888:Real-Time Polymerase Chain Reaction; D014184:Transplantation, Homologous
chemical_list: D011509:Proteoglycans
keywords: ESM-1; Kidney transplantation; Rat; Rejection