title: Validated spectrofluorimetric method for the determination of tamsulosin in spiked human urine, pure and pharmaceutical preparations.
abstract: A novel, sensitive and selective spectrofluorimetric method was developed for the determination of tamsulosin in spiked human urine and pharmaceutical preparations. The proposed method is based on the reaction of tamsulosin with 1-dimethylaminonaphthalene-5-sulfonyl chloride in carbonate buffer pH 10.5 to yield a highly fluorescent derivative. The described method was validated and the analytical parameters of linearity, limit of detection (LOD), limit of quantification (LOQ), accuracy, precision, recovery and robustness were evaluated. The proposed method showed a linear dependence of the fluorescence intensity on drug concentration over the range 1.22 × 10(-7) to 7.35 × 10(-6)  M. LOD and LOQ were calculated as 1.07 × 10(-7) and 3.23 × 10(-7)  M, respectively. The proposed method was successfully applied for the determination of tamsulosin in pharmaceutical preparations and the obtained results were in good agreement with those obtained using the reference method.
authors.affiliation: Department of Chemistry, Faculty of Science and Letters, Namik Kemal University, Tekirdag, Turkey.
authors.forename: A
authors.initials: A
authors.lastname: Karasakal
authors.forename: S T
authors.initials: ST
authors.lastname: Ulu
mesh_terms: D058668:Adrenergic alpha-1 Receptor Antagonists / Q000652:urine*; D000328:Adult; D002214:Capsules / Q000032:analysis; D005260:Female; D006801:Humans; D057230:Limit of Detection; D008875:Middle Aged; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013449:Sulfonamides / Q000032:analysis* / Q000652:urine; D000077409:Tamsulosin
chemical_list: D058668:Adrenergic alpha-1 Receptor Antagonists; D002214:Capsules; D013449:Sulfonamides; D000077409:Tamsulosin
keywords: dansyl chloride; derivatization; human urine; method validation