title: The effect of erythropoietin on autologous stem cell-mediated bone regeneration.
abstract: Mesenchymal stem cells (MSCs) although used for bone tissue engineering are limited by the requirement of isolation and culture prior to transplantation. Our recent studies have shown that biomaterial implants can be engineered to facilitate the recruitment of MSCs. In this study, we explore the ability of these implants to direct the recruitment and the differentiation of MSCs in the setting of a bone defect. We initially determined that both stromal derived factor-1alpha (SDF-1α) and erythropoietin (Epo) prompted different degrees of MSC recruitment. Additionally, we found that Epo and bone morphogenetic protein-2 (BMP-2), but not SDF-1α, triggered the osteogenic differentiation of MSCs in vitro. We then investigated the possibility of directing autologous MSC-mediated bone regeneration using a murine calvaria model. Consistent with our in vitro observations, Epo-releasing scaffolds were found to be more potent in bridging the defect than BMP-2 loaded scaffolds, as determined by computed tomography (CT) scanning, fluorescent imaging and histological analyses. These results demonstrate the tremendous potential, directing the recruitment and differentiation of autologous MSCs has in the field of tissue regeneration.
authors.affiliation: Bioengineering Department, University of Texas Southwestern Medical Center and The University of Texas at Arlington, Arlington, TX 76019, USA.
authors.forename: Ashwin M
authors.initials: AM
authors.lastname: Nair
authors.forename: Yi-Ting
authors.initials: YT
authors.lastname: Tsai
authors.forename: Krishna M
authors.initials: KM
authors.lastname: Shah
authors.forename: Jinhui
authors.initials: J
authors.lastname: Shen
authors.forename: Hong
authors.initials: H
authors.lastname: Weng
authors.forename: Jun
authors.initials: J
authors.lastname: Zhou
authors.forename: Xiankai
authors.initials: X
authors.lastname: Sun
authors.forename: Ramesh
authors.initials: R
authors.lastname: Saxena
authors.forename: Joseph
authors.initials: J
authors.lastname: Borrelli
authors.forename: Liping
authors.initials: L
authors.lastname: Tang
mesh_terms: D000818:Animals; D001861:Bone Regeneration / Q000187:drug effects*; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D018925:Chemokines / Q000494:pharmacology; D002633:Chemotaxis / Q000187:drug effects; D004921:Erythropoietin / Q000494:pharmacology*; D007150:Immunohistochemistry; D020007:Implants, Experimental; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D010012:Osteogenesis / Q000187:drug effects; D012886:Skull / Q000000981:diagnostic imaging / Q000187:drug effects / Q000473:pathology; D054457:Tissue Scaffolds / Q000737:chemistry; D014182:Transplantation, Autologous; D055114:X-Ray Microtomography
chemical_list: D018925:Chemokines; D004921:Erythropoietin
keywords: Autologous stem cells; Bone morphogenetic protein-2; Bone tissue engineering; Erythropoietin; Scaffold; Stromal derived factor-1α