title: Death-domain associated protein-6 (DAXX) mediated apoptosis in hantavirus infection is counter-balanced by activation of interferon-stimulated nuclear transcription factors.
abstract: Hantaviruses are negative strand RNA species that replicate predominantly in the cytoplasm. They also activate numerous cellular responses, but their involvement in nuclear processes is yet to be established. Using human umbilical vein endothelial cells (HUVECs), this study investigates the molecular finger-print of nuclear transcription factors during hantavirus infection. The viral-replication-dependent activation of pro-myelocytic leukemia protein (PML) was followed by subsequent localization in nuclear bodies (NBs). PML was also found in close proximity to activated Sp100 nuclear antigen and interferon-stimulated gene 20 kDa protein (ISG-20), but co-localization with death-domain associated protein-6 (DAXX) was not observed. These data demonstrate that hantavirus triggers PML activation and localization in NBs in the absence of DAXX-PLM-NB co-localization. The results suggest that viral infection interferes with DAXX-mediated apoptosis, and expression of interferon-activated Sp100 and ISG-20 proteins may indicate intracellular intrinsic antiviral attempts.
authors.affiliation: Whittemore Peterson Institute, University of Nevada-Reno, Reno, USA. sv.khaiboullina@gmail.com
authors.forename: Svetlana F
authors.initials: SF
authors.lastname: Khaiboullina
authors.forename: Sergey P
authors.initials: SP
authors.lastname: Morzunov
authors.forename: Sergei V
authors.initials: SV
authors.lastname: Boichuk
authors.forename: András
authors.initials: A
authors.lastname: Palotás
authors.forename: Stephen
authors.initials: S
authors.lastname: St Jeor
authors.forename: Vincent C
authors.initials: VC
authors.lastname: Lombardi
authors.forename: Albert A
authors.initials: AA
authors.lastname: Rizvanov
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics / Q000378:metabolism*; D034961:Antigens, Nuclear / Q000235:genetics / Q000378:metabolism*; D017209:Apoptosis; D001324:Autoantigens / Q000235:genetics / Q000378:metabolism*; D056970:Co-Repressor Proteins; D042783:Endothelial Cells / Q000276:immunology / Q000821:virology; D005092:Exonucleases / Q000235:genetics / Q000378:metabolism*; D005095:Exoribonucleases; D005786:Gene Expression Regulation; D018778:Hantavirus Infections / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D007372:Interferons / Q000494:pharmacology; D045586:Intranuclear Inclusion Bodies / Q000378:metabolism; D018832:Molecular Chaperones; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D000071156:Promyelocytic Leukemia Protein; D025842:SUMO-1 Protein / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D025521:Tumor Suppressor Proteins / Q000235:genetics / Q000378:metabolism; D014471:Umbilical Veins / Q000166:cytology / Q000821:virology
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D034961:Antigens, Nuclear; D001324:Autoantigens; D056970:Co-Repressor Proteins; C106985:DAXX protein, human; D018832:Molecular Chaperones; D009687:Nuclear Proteins; D000071156:Promyelocytic Leukemia Protein; D025842:SUMO-1 Protein; D014157:Transcription Factors; D025521:Tumor Suppressor Proteins; C066642:SP100 protein, human; C070576:PML protein, human; D007372:Interferons; D005092:Exonucleases; D005095:Exoribonucleases; C112346:ISG20 protein, human
keywords: DAXX; HUVEC; Hantavirus; ISG-20; PML; SUMO; Sp100