title: Saponins-rich fraction of Calotropis procera leaves elicit no antitrypanosomal activity in a rat model.
abstract: OBJECTIVE
  To examine the in vitro and in vivo anti-Trypanosoma evansi (T. evansi ) activity of saponins-rich fraction of Calotropis procera (cpsf) leaves as well as the effect of the fraction on the parasite-induced anemia.
  
  
  METHODS
  A 60-minutes time course experiment was conducted with various concentrations of the fraction using a 96-well microtiter plate technique, and subsequently used to treat experimentally T. evansi infected rats at 100 and 200 mg/kg body weight. Index of anemia was analyzed in all animals during the experiment.
  
  
  RESULTS
  The cpsf did not demonstrate an in vitro antitrypanosomal activity. Further, the cpsf treatments did not significantly (P>0.05) keep the parasites lower than the infected untreated groups. At the end of the experiment, all T. evansi infected rats developed anemia whose severity was not significantly (P>0.05) ameliorated by the cpsf treatment.
  
  
  CONCLUSIONS
  It was concluded that saponins derived from Calotropis procera leaves could not elicit in vitro and in vivo activities against T. evansi.
authors.affiliation: Department of Biochemistry, Zaria, Nigeria. mauwalibrahim@gmail.com
authors.forename: Mohammed Auwal
authors.initials: MA
authors.lastname: Ibrahim
authors.forename: Abubakar Babando
authors.initials: AB
authors.lastname: Aliyu
authors.forename: Kayode
authors.initials: K
authors.lastname: Meduteni
authors.forename: Isa
authors.initials: I
authors.lastname: Yunusa
mesh_terms: D000818:Animals; D031107:Calotropis / Q000737:chemistry*; D004195:Disease Models, Animal; D066298:In Vitro Techniques; D007840:Latex / Q000494:pharmacology; D008517:Phytotherapy / Q000379:methods; D010936:Plant Extracts / Q000494:pharmacology; D018515:Plant Leaves; D051381:Rats; D012503:Saponins / Q000494:pharmacology; D014345:Trypanosoma / Q000187:drug effects; D014352:Trypanosomiasis / Q000188:drug therapy / Q000469:parasitology*
chemical_list: D007840:Latex; D010936:Plant Extracts; D012503:Saponins
keywords: Anemia; Asclepiadaceae; Calotropis procera; Trypanosoma evansi; saponins