title: Urinary bisphenol A concentrations and cytochrome P450 19 A1 (Cyp19) gene expression in ovarian granulosa cells: an in vivo human study.
abstract: BACKGROUND
  Exposure to bisphenol A (BPA), a chemical widely used in consumer products, has been associated with in vitro Cyp19 gene expression.
  
  
  OBJECTIVE
  To evaluate an in vivo human model of Cyp19 gene expression in granulosa cells.
  
  
  METHODS
  A subset of an ongoing prospective cohort study of women undergoing in vitro fertilization (IVF) at Massachusetts General Hospital.
  
  
  METHODS
  Mixed effect models were used to evaluate the association of urinary BPA concentrations with granulosa cell Cyp19 mRNA expression.
  
  
  RESULTS
  In 61 women undergoing 76 IVF cycles, adjusted changes in mean Cyp19 expression (β estimate (95% CI)) for quartiles 2, 3 and 4 as compared to the lowest quartile were: -0.97 (-2.22, 0.28); -0.97 (-2.18, 0.24) and -0.38 (-1.58, 0.82).
  
  
  CONCLUSIONS
  An in vivo model for evaluation of Cyp19 gene expression was developed for use in epidemiologic studies. In this pilot study, we found no statistically significant linear association between urinary BPA concentrations and Cyp19 expression.
authors.affiliation: Department of Environmental Health, Harvard School of Public Health, Boston, MA, USA.
authors.forename: Shelley
authors.initials: S
authors.lastname: Ehrlich
authors.forename: Paige L
authors.initials: PL
authors.lastname: Williams
authors.forename: Russ
authors.initials: R
authors.lastname: Hauser
authors.forename: Stacey A
authors.initials: SA
authors.lastname: Missmer
authors.forename: Jackye
authors.initials: J
authors.lastname: Peretz
authors.forename: Antonia M
authors.initials: AM
authors.lastname: Calafat
authors.forename: Jodi A
authors.initials: JA
authors.lastname: Flaws
mesh_terms: D000328:Adult; D001141:Aromatase / Q000235:genetics*; D001559:Benzhydryl Compounds / Q000652:urine*; D004785:Environmental Pollutants / Q000652:urine*; D005260:Female; D005307:Fertilization in Vitro; D015870:Gene Expression; D006107:Granulosa Cells / Q000378:metabolism*; D006801:Humans; D010636:Phenols / Q000652:urine*; D012333:RNA, Messenger / Q000378:metabolism; D055815:Young Adult; D000098826:Bisphenol A Compounds
chemical_list: D001141:Aromatase; D001559:Benzhydryl Compounds; D004785:Environmental Pollutants; D010636:Phenols; D012333:RNA, Messenger; C561410:CYP19A1 protein, human; C006780:bisphenol A; D000098826:Bisphenol A Compounds
keywords: Bisphenol A; Cyp19; Fertility; Follicular fluid; Gene expression; Human; Ovary, granulosa cells; P450 aromatase