title: [Effects of combination of Salvia and Ligustrazine on TNF-α and TGF-β1 in serum and BALF of rats with pulmonary fibrosis].
abstract: OBJECTIVE
  To observe the effects of Salvia combined with Ligustrazine on the TNF-α and TGF-β1 in sera and bronchoalveolar lavage fluid (BALF) of rats with pulmonary fibrosis induced by bleomycin (BLM).
  
  
  METHODS
  Male SD rats (n=90) were randomly assigned into 6 groups: normal saline (NS) group, BLM group, dexamethasone (DXM) group and three Chinese traditional herbal groups (C1: small dose group, C2: medium dose group, C3: large dose group). Rats were challenged intratracheally with BLM to establish pulmonary fibrosis rat models, while the NS group with 0.9% NaCl solution. Rats were given intra-peritoneal injections of normal saline (both the NS group and the BLM group), dexamethasone (the DXM group), or Salvia combined with Ligustrazine (the C1, C2, C3 groups). At the same time, 5 rats from the 6 groups were sacrificed at day 7, 14 and 28 after administration respectively. HE and Masson staining were performed to observe the extent of alveolitis and fibrosis; the levels of TNF-α and TGF-β1 in both sera and BALF were detected by ELISA.
  
  
  RESULTS
  Compared with NS group, a great deal of inflammatory cell infiltration into the alveolar was observed in BLM group at day 7, the alveolitis was lessened at day 14, while fibrosis was found; at day 28, the alveolitis became relieved and the fibrosis developed seriously. The extent of alveolitis and pulmonary fibrosis in DXM group was milder than that in BLM group at day 7, 14 and 28 (P<0.05). Compared with BLM group, the extent of pulmonary fibrosis in group C1 didn't remarkably change (P>0.05). The extent of alveolitis and pulmonary fibrosis in group C2 and C3 was significantly milder than that in BLM group at day 14, and the extent of pulmonary fibrosis in group C2 and C3 was remarkably reduced at day 28 (P<0.05). Compared with NS group, the levels of TNF-α and TGF-β1 in both sera and BALF of the other groups were obviously higher (P<0.05). Compared with BLM group, the levels in DXM, C2 and C3 groups were lower at day 7, 14, 28 (P<0.05). Compared with DXM group, the levels in C1, C2 and C3 groups were lower at day 7, 14, 28 (P<0.05), and the difference between C2 and C3 groups was statistically significant (P>0.05). The levels of of TNF-α and TGF-β1 were consistent in rat sera and BALF.
  
  
  CONCLUSIONS
  Medium and large doses of Salvia combined with Ligustrazine could abate the alveolitis and ameliorate the extent of pulmonary fibrosis by decreasing the levels of TNF-α and TGF-β1 in both serum and BALF.
authors.affiliation: Department of Respiratory Medicine, Affiliated Hospital of Luzhou Medical College, Luzhou 646000, China.
authors.forename: Chengliang
authors.initials: C
authors.lastname: Huang
authors.forename: Yanyan
authors.initials: Y
authors.lastname: Li
authors.forename: Xianming
authors.initials: X
authors.lastname: Fan
authors.forename: Wenjun
authors.initials: W
authors.lastname: Wang
authors.forename: Xiaoqin
authors.initials: X
authors.lastname: Zhan
mesh_terms: D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry*; D004195:Disease Models, Animal; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000494:pharmacology*; D054990:Idiopathic Pulmonary Fibrosis / Q000188:drug therapy / Q000378:metabolism*; D008168:Lung / Q000187:drug effects / Q000473:pathology; D008297:Male; D011719:Pyrazines / Q000008:administration & dosage / Q000494:pharmacology*; D051381:Rats; D027544:Salvia / Q000737:chemistry*; D053773:Transforming Growth Factor beta1 / Q000097:blood*; D014409:Tumor Necrosis Factor-alpha / Q000097:blood*
chemical_list: D004365:Drugs, Chinese Herbal; D011719:Pyrazines; D053773:Transforming Growth Factor beta1; D014409:Tumor Necrosis Factor-alpha; C017953:tetramethylpyrazine