title: Antibacterial and anti-adhesion effects of the silver nanoparticles-loaded poly(L-lactide) fibrous membrane.
abstract: The complications of tendon injury are frequently compromised by peritendinous adhesions and tendon sheath infection. Physical barriers for anti-adhesion may increase the incidence of postoperative infection. This study was designed to evaluate the potential of silver nanoparticles (AgNPs)-loaded poly(L-lactide) (PLLA) electrospun fibrous membranes to prevent adhesion formation and infection. Results of an in vitro drug release study showed that a burst release was followed by sustained release from electrospun fibrous membranes with a high initial silver content. Fewer fibroblasts adhered to and proliferated on the AgNP-loaded PLLA electrospun fibrous membranes compared with pure PLLA electrospun fibrous membrane. In the antibacterial test, the AgNP-loaded PLLA electrospun fibrous membranes can prevent the adhesion of Gram-positive Staphylococcus aureus and Staphylococcus epidermidis and Gram-negative Pseudomonas aeruginosa. Taken together, these results demonstrate that AgNP-loaded PLLA electrospun fibrous membranes have the convenient practical medical potential of reduction of infection and adhesion formation after tendon injury.
authors.affiliation: Department of Orthopaedics, Shanghai Sixth People's Hospital, Shanghai Jiao Tong University School of Medicine, 600 Yishan Road, Shanghai 200233, PR China.
authors.forename: Shen
authors.initials: S
authors.lastname: Liu
authors.forename: Jingwen
authors.initials: J
authors.lastname: Zhao
authors.forename: Hongjiang
authors.initials: H
authors.lastname: Ruan
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Tianyi
authors.initials: T
authors.lastname: Wu
authors.forename: Wenguo
authors.initials: W
authors.lastname: Cui
authors.forename: Cunyi
authors.initials: C
authors.lastname: Fan
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001422:Bacterial Adhesion / Q000187:drug effects*; D016923:Cell Death / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D007477:Ions; D008567:Membranes, Artificial; D053768:Metal Nanoparticles / Q000737:chemistry*; D051379:Mice; D008826:Microbial Sensitivity Tests; D018613:Microscopy, Confocal; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D057139:Nanofibers / Q000737:chemistry* / Q000648:ultrastructure; D011091:Polyesters / Q000494:pharmacology*; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000648:ultrastructure; D012834:Silver / Q000494:pharmacology*; D013210:Staphylococcus / Q000187:drug effects / Q000648:ultrastructure
chemical_list: D000900:Anti-Bacterial Agents; D007477:Ions; D008567:Membranes, Artificial; D011091:Polyesters; D012834:Silver; C033616:poly(lactide)
keywords: Bacteria; Electrospun fibrous membrane; Infection; Peritendinous adhesions; Silver nanoparticle