title: Fragmentation characterization and differentiation of isomeric diglycosyl flavonoids using ultra-high-performance liquid chromatography electrospray ionization quadrupole time-of-flight tandem mass spectrometry in negative ion mode.
abstract: Flavonoids exhibit a wide range of biological activities and the fragmentation characterization and differentiation of isomeric diglycosyl flavonoids is an important area of research. In this study, ultra-high-performance liquid chromatography electrospray ionization quadrupole time-of-flight tandem mass spectrometry was used to identify two pairs of isomeric diglycosyl flavonoids: naringin/narirutin and neohesperidin/hesperidin. The two pairs of flavonoids were identified by product ion tandem mass spectra of the precursor ions [M-H]-. The results showed that characteristic product ions distinguishing flavonoids glycosylated with rhamnosyl-(alpha1 --> 2)-glucosyl from isomeric flavonoids glycosylated with rhamnosyl-(alpha1 --> 6)-glucosyl were the precursor ion [M-H]- and its characteristic product ions Y0- and [0.2X0-H]-.
authors.affiliation: State Forestry Administration Key Open Laboratory, International Centre for Bamboo and Rattan, Beijing 100102, PR China.
authors.forename: Xuefeng
authors.initials: X
authors.lastname: Guo
authors.forename: Yongde
authors.initials: Y
authors.lastname: Yue
authors.forename: Feng
authors.initials: F
authors.lastname: Tang
authors.forename: Jia
authors.initials: J
authors.lastname: Sun
authors.forename: Jin
authors.initials: J
authors.lastname: Wang
authors.forename: Xi
authors.initials: X
authors.lastname: Yao
authors.forename: Hang
authors.initials: H
authors.lastname: Xun
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D044950:Flavanones / Q000032:analysis / Q000737:chemistry*; D007536:Isomerism; D000432:Methanol / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D044950:Flavanones; D000432:Methanol