title: Positioning of integrin β1, caveolin-1 and focal adhesion kinase on the adhered membrane of spreading cells.
abstract: We have investigated the relationship between the spreading of anchorage-dependent cells and the surface-density distribution of plasma membrane adhesion proteins. The surface positioning and density of integrin β1, caveolin-1 (cav-1), the phosphorylated caveolin-1 (p-cav-1) and the focal adhesion kinase (FAK) located on the adhering cell membrane (ACM) of HUVEC cells was studied. Imaging with TIRF microscopy was used, which enabled us to observe a few-nanometers-thin section of the cell above the plasma membrane in combination with image-based analyses. Integrin β1 and cav-1 have spatial interdependence on the ACM. Cells treated with substances that act on cell spreading caused changes in the size of the ACM area, as well as a redistribution of several proteins under investigation. Changes to the ACM area correlated positively with those to the surface density of the cav-1. The high integrin β1 and the low cav-1 surface density, and vice versa, following the treatments show that the presence of one of them not only spatially excludes, but also reduces, the occurrence of the other protein on the ACM, which indicates a regulative mechanism between integrin β1 and cav-1.
authors.affiliation: Institute of Biophysics, Faculty of Medicine, University of Ljubljana, Ljubljana, Slovenia.
authors.forename: Spela Zemljič
authors.initials: SZ
authors.lastname: Jokhadar
authors.forename: Janja
authors.initials: J
authors.lastname: Majhenc
authors.forename: Saša
authors.initials: S
authors.lastname: Svetina
authors.forename: Urška
authors.initials: U
authors.lastname: Batista
mesh_terms: D019086:Bridged Bicyclo Compounds, Heterocyclic / Q000494:pharmacology; D051242:Caveolin 1 / Q000378:metabolism*; D002448:Cell Adhesion / Q000187:drug effects; D002462:Cell Membrane / Q000378:metabolism; D002712:Chlorides / Q000494:pharmacology; D051416:Focal Adhesion Protein-Tyrosine Kinases / Q000378:metabolism*; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D019012:Integrin beta1 / Q000378:metabolism*; D017895:Manganese Compounds / Q000494:pharmacology; D008856:Microscopy, Fluorescence; D010766:Phosphorylation / Q000187:drug effects; D053778:Thiazolidines / Q000494:pharmacology
chemical_list: D019086:Bridged Bicyclo Compounds, Heterocyclic; D051242:Caveolin 1; D002712:Chlorides; D019012:Integrin beta1; D017895:Manganese Compounds; D053778:Thiazolidines; D051416:Focal Adhesion Protein-Tyrosine Kinases; C025340:manganese chloride; C037067:latrunculin A
keywords: caveolin-1; cytoskeleton; integrin β1; surface density