title: A clinical study of HBsAg-activated dendritic cells and cytokine-induced killer cells during the treatment for chronic hepatitis B.
abstract: We aim to study the therapeutic effects of HBsAg-activated DCs and cytokine-induced killer (CIK) cells as adoptive immunotherapy in patients with Chronic Hepatitis B (CHB). Autologous HBsAg-activated DC-CIK cells were infused into patients with CHB to evaluate their effect on HBV-DNA, HBsAg, ALT, etc. The viral load in the treatment group decreased significantly (P < 0.001), while that in the control group did not decrease (P > 0.05). Twenty-one patients (63.6% efficiency) in the treatment group had a viral response (≥2 log decrease in viral load), while four patients (14.8% efficiency) from the control group had a viral response. There were significant differences in the viral responses of the two groups (the control group 63.6% versus the control group 14.8%, P < 0.001). We concluded that the immunity was enhanced after HBsAg activation in DCs and CIK cells. Reinfusion of autologous HBsAg-activated DC-CIK cells inhibited HBV proliferation in 21 of 33 (63.6%) patients.
authors.affiliation: Zhengzhou Institute of Hepatological and Gastrointestinal Disease, Zhengzhou People's Hospital, Zhengzhou, China.
authors.forename: Y-J
authors.initials: YJ
authors.lastname: Ma
authors.forename: M
authors.initials: M
authors.lastname: He
authors.forename: J-A
authors.initials: JA
authors.lastname: Han
authors.forename: L
authors.initials: L
authors.lastname: Yang
authors.forename: X-Y
authors.initials: XY
authors.lastname: Ji
mesh_terms: D000293:Adolescent; D019264:Adoptive Transfer / Q000379:methods; D000328:Adult; D002478:Cells, Cultured; D055612:Cytokine-Induced Killer Cells / Q000276:immunology* / Q000637:transplantation; D003713:Dendritic Cells / Q000276:immunology* / Q000637:transplantation; D005260:Female; D006514:Hepatitis B Surface Antigens / Q000276:immunology*; D006515:Hepatitis B virus / Q000276:immunology; D019694:Hepatitis B, Chronic / Q000276:immunology* / Q000628:therapy / Q000821:virology; D006801:Humans; D008297:Male; D008875:Middle Aged; D013997:Time Factors; D016896:Treatment Outcome; D019562:Viral Load / Q000276:immunology; D014779:Virus Replication / Q000276:immunology; D055815:Young Adult
chemical_list: D006514:Hepatitis B Surface Antigens