title: Fluorescent hydroxylamine derived from the fragmentation of PAMAM dendrimers for intracellular hypochlorite recognition.
abstract: Herein, a promising sensing approach based on the structure fragmentation of poly(amidoamine) (PAMAM) dendrimers for the selective detection of intracellular hypochlorite (OCl(-)) is reported. PAMAM dendrimers were easily disrupted by a cascade of oxidations in the tertiary amines of the dendritic core to produce an unsaturated hydroxylamine with blue fluorescence. Specially, the novel fluorophore was only sensitive to OCl(-), one of reactive oxygen species (ROS), resulting in an irreversible fluorescence turn-off. The fluorescent hydroxylamine was selectively oxidised by OCl(-) to form a labile oxoammonium cation that underwent further degradation. Without using any troublesomely synthetic steps, the novel sensing platform based on the fragmentation of PAMAM dendrimers, can be applied to detect OCl(-) in macrophage cells. The results suggest that the sensing approach may be useful for the detection of intracellular OCl(-) with minimal interference from biological matrixes.
authors.affiliation: Center for Nanomedicine Research, National Health Research Institutes, 35 Keyan Road Zhunan, 35053 Miaoli, Taiwan.
authors.forename: Te-Haw
authors.initials: TH
authors.lastname: Wu
authors.forename: Ching-Ping
authors.initials: CP
authors.lastname: Liu
authors.forename: Chih-Te
authors.initials: CT
authors.lastname: Chien
authors.forename: Shu-Yi
authors.initials: SY
authors.lastname: Lin
mesh_terms: D000588:Amines / Q000737:chemistry; D050091:Dendrimers / Q000138:chemical synthesis* / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry*; D019811:Hydroxylamine / Q000138:chemical synthesis* / Q000737:chemistry; D006997:Hypochlorous Acid; D017382:Reactive Oxygen Species
chemical_list: D000588:Amines; D050091:Dendrimers; D005456:Fluorescent Dyes; D017382:Reactive Oxygen Species; D019811:Hydroxylamine; D006997:Hypochlorous Acid
keywords: dendrimers; fluorescence; hydroxylamine; hypochlorite; structure fragmentation