title: Immune response of calves inoculated with proteins of Anaplasma marginale bound to an immunostimulant complex.
abstract: Despite our current knowledge of the immunology, pathology, and genetics of Anaplasma marginale, prevention in cattle is currently based on old standbys, including live attenuated vaccines, antibiotic treatment, and maintaining enzootic stability in cattle herds. In the present study, we evaluated the use of an immunostimulant complex (ISCOMATRIX) adjuvant, associated with a pool of recombinant major surface proteins (rMSP1a, rMSP1b, rMSP4 and rMSP5) to improve the humoral immune response triggered in calves mainly by IgG2. Ten calves were divided in three groups: 4 calves were inoculated with the ISCOMATRIX/rMSPs (G1); 2 calves were inoculated with ISCOMATRIX adjuvant (G2); and 4 calves received saline (G3). Three inoculations were administered at 21-day intervals. In G1, the calves showed significant increases in total IgG, IgG1 and IgG2 levels 21 days after the second inoculation, compared to the control group (p < 0.05), and G1 calves remained above the cut-off value 28 days after the third inoculation (p < 0.05). The post-immunized sera from calves in G1 reacted specifically for each of the rMSPs used. In conclusion, the ISCOMATRIX/rMSPs induced antigen-specific seroconversion in calves. Therefore, additional testing to explore the protection induced by rMSPs, both alone and in conjunction with proteins previously identified as subdominant epitopes, is warranted.
authors.affiliation: Department of Preventive Veterinary Medicine, Londrina State University, Londrina, PR, Brazil.
authors.forename: Marcela Ribeiro
authors.initials: MR
authors.lastname: Gasparini
authors.forename: Rafael Felipe da Costa
authors.initials: RF
authors.lastname: Vieira
authors.forename: Denise Amaral Gomes do
authors.initials: DA
authors.lastname: Nascimento
authors.forename: João Luis
authors.initials: JL
authors.lastname: Garcia
authors.forename: Odilon
authors.initials: O
authors.lastname: Vidotto
authors.forename: Marilda Carlos
authors.initials: MC
authors.lastname: Vidotto
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage; D042322:Anaplasma marginale; D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000502:physiology*; D002417:Cattle / Q000276:immunology*; D002784:Cholesterol / Q000008:administration & dosage; D004338:Drug Combinations; D010743:Phospholipids / Q000008:administration & dosage; D012503:Saponins / Q000008:administration & dosage
chemical_list: D000276:Adjuvants, Immunologic; D001425:Bacterial Outer Membrane Proteins; D004338:Drug Combinations; C493786:ISCOMATRIX; D010743:Phospholipids; D012503:Saponins; D002784:Cholesterol