title: [An in vitro evaluation of the effect of carboxymethyl chitosan and its composites against Enterococcus faecalis in the root canal].
abstract: OBJECTIVE
  To evaluate the bacteriostasis effect of carboxymethyl chitosan and its composites on intracanal E. faecalis.
  
  
  METHODS
  The pattern of E. faecalis infecting root canal was established, and then divided into 4 groups(A, B, C and D).They were filled with 2% chlorhexidine solution, 140 mg/mL mixture of carboxymethyl chitosan and chlorhexidine solution, 5 mg/mL carboxymethyl chitosan solution and calcium hydroxide paste respectively and incubated for 7 days. Samples obtained before and after the intracanal medication were plated onto BHI media to determine the colony-forming units (CFU/mL) after 48 hours. The data were analysed using SPSS 13.0 software package.
  
  
  RESULTS
  Before intracanal medication, the variance of bacterial counts were not significantly different (P>0.05). After medication, the four groups showed significant difference in bacterial counts immediately(P<0.05).The antimicrobial effects of A and B group were better than group C and D.
  
  
  CONCLUSIONS
  The antibiotic activity of 2% chlorhexidine solution and 140 mg/L mixture of carboxymethyl chitosan and chlorhexidine solution to E.faecalis were better. Supported by Key Science and Technology Project of Liaoning Province(2010225001).
authors.affiliation: Department of Endodontics, China Medical University. Shenyang, Liaoning Province, China. sxl266@163.com
authors.forename: Xue-lian
authors.initials: XL
authors.lastname: Song
authors.forename: Ying-ying
authors.initials: YY
authors.lastname: Sun
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.forename: Qing
authors.initials: Q
authors.lastname: Chen
authors.forename: Xin-xin
authors.initials: XX
authors.lastname: Bi
authors.forename: Jing-tao
authors.initials: JT
authors.lastname: Yu
mesh_terms: D000900:Anti-Bacterial Agents; D058491:Bacterial Load; D002126:Calcium Hydroxide; D048271:Chitosan / Q000031:analogs & derivatives; D002710:Chlorhexidine; D003786:Dental Pulp Cavity; D013293:Enterococcus faecalis; D006801:Humans; D066298:In Vitro Techniques; D012388:Root Canal Irrigants; D012390:Root Canal Therapy
chemical_list: D000900:Anti-Bacterial Agents; D012388:Root Canal Irrigants; C514968:carboxymethyl-chitosan; D048271:Chitosan; D002126:Calcium Hydroxide; D002710:Chlorhexidine