title: Evaluation of nifurtimox treatment of chronic Chagas disease by means of several parasitological methods.
abstract: Currently, evaluation of drug efficacy for Chagas disease remains a controversial issue with no consensus. In this work, we evaluated the parasitological efficacy of Nifurtimox treatment in 21 women with chronic Chagas disease from an area of endemicity in Chile who were treated according to current protocols. Under pre- and posttherapy conditions, blood (B) samples and xenodiagnosis (XD) samples from these patients were subjected to analysis by real-time PCR targeting the nuclear satellite DNA of Trypanosoma cruzi (Sat DNA PCR-B, Sat DNA PCR-XD) and by PCR targeting the minicircle of kinetoplast DNA of T. cruzi (kDNA PCR-B, kDNA PCR-XD) and by T. cruzi genotyping using hybridization minicircle tests in blood and fecal samples of Triatoma infestans feed by XD. In pretherapy, kDNA PCR-B and kDNA PCR-XD detected T. cruzi in 12 (57%) and 18 (86%) cases, respectively, whereas Sat DNA quantitative PCR-B (qPCR-B) and Sat DNA qPCR-XD were positive in 18 cases (86%) each. Regarding T. cruzi genotype analysis, it was possible to observe in pretherapy the combination of TcI, TcII, and TcV lineages, including mixtures of T. cruzi strains in most of the cases. At 13 months posttherapy, T. cruzi DNA was detectable in 6 cases (29.6%) and 4 cases (19.1%) by means of Sat DNA PCR-XD and kDNA PCR-XD, respectively, indicating treatment failure with recovery of live parasites refractory to chemotherapy. In 3 cases, it was possible to identify persistence of the baseline genotypes. The remaining 15 baseline PCR-positive cases gave negative results by all molecular and parasitological methods at 13 months posttreatment, suggesting parasite response. Within this follow-up period, kDNA PCR-XD and Sat DNA qPCR-XD proved to be more sensitive tools for the parasitological evaluation of the efficacy of Nifurtimox treatment than the corresponding PCR methods performed directly from blood samples.
authors.affiliation: Laboratorio de Parasitología Básico-Clínico, Programa de Biología Celular y Molecular, Instituto de Ciencias Biomédicas, Facultad de Medicina, Universidad de Chile, Santiago, Chile.
authors.forename: Catalina
authors.initials: C
authors.lastname: Muñoz
authors.forename: Inés
authors.initials: I
authors.lastname: Zulantay
authors.forename: Werner
authors.initials: W
authors.lastname: Apt
authors.forename: Sylvia
authors.initials: S
authors.lastname: Ortiz
authors.forename: Alejandro G
authors.initials: AG
authors.lastname: Schijman
authors.forename: Margarita
authors.initials: M
authors.lastname: Bisio
authors.forename: Valentina
authors.initials: V
authors.lastname: Ferrada
authors.forename: Cinthya
authors.initials: C
authors.lastname: Herrera
authors.forename: Gabriela
authors.initials: G
authors.lastname: Martínez
authors.forename: Aldo
authors.initials: A
authors.lastname: Solari
mesh_terms: D000328:Adult; D000818:Animals; D014355:Chagas Disease / Q000175:diagnosis / Q000188:drug therapy* / Q000469:parasitology; D002908:Chronic Disease; D016054:DNA, Protozoan / Q000302:isolation & purification*; D005260:Female; D060005:Genotyping Techniques; D006801:Humans; D008875:Middle Aged; D009547:Nifurtimox / Q000627:therapeutic use*; D016133:Polymerase Chain Reaction / Q000379:methods; D016896:Treatment Outcome; D014344:Trypanocidal Agents / Q000627:therapeutic use*; D014349:Trypanosoma cruzi / Q000187:drug effects* / Q000502:physiology
chemical_list: D016054:DNA, Protozoan; D014344:Trypanocidal Agents; D009547:Nifurtimox