title: Physico-chemical/biological properties of tripolyphosphate cross-linked chitosan based nanofibers.
abstract: In this study, chitosan-PEO blend, prepared in a 15 M acetic acid, was electrospun into nanofibers (~78 nm diameter) with bead free morphology. While investigating physico-chemical parameters of blend solutions, effect of yield stress on chitosan based nanofiber fabrication was clearly evidenced. Architectural stability of nanofiber mat in aqueous medium was achieved by ionotropic cross-linking of chitosan by tripolyphosphate (TPP) ions. The TPP cross-linked nanofiber mat showed swelling up to ~300% in 1 h and ~40% degradation during 30 day study period. 3T3 fibroblast cells showed good attachment, proliferation and viability on TPP treated chitosan based nanofiber mats. The results indicate non-toxic nature of TPP cross-linked chitosan based nanofibers and their potential to be explored as a tissue engineering matrix.
authors.affiliation: School of Medical Science and Technology, Indian Institute of Technology Kharagpur, Kharagpur-721302, India.
authors.forename: Soumi Dey
authors.initials: SD
authors.lastname: Sarkar
authors.forename: Brooke L
authors.initials: BL
authors.lastname: Farrugia
authors.forename: Tim R
authors.initials: TR
authors.lastname: Dargaville
authors.forename: Santanu
authors.initials: S
authors.lastname: Dhara
mesh_terms: D016475:3T3 Cells; D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D055598:Chemical Phenomena / Q000187:drug effects*; D048271:Chitosan / Q000494:pharmacology*; D003432:Cross-Linking Reagents / Q000494:pharmacology*; D003460:Crystallization; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000648:ultrastructure; D051379:Mice; D057139:Nanofibers / Q000737:chemistry* / Q000648:ultrastructure; D011092:Polyethylene Glycols / Q000737:chemistry; D011122:Polyphosphates / Q000494:pharmacology*; D012996:Solutions; D017550:Spectroscopy, Fourier Transform Infrared; D013696:Temperature; D013818:Thermogravimetry; D014961:X-Ray Diffraction
chemical_list: D003432:Cross-Linking Reagents; D011122:Polyphosphates; D012996:Solutions; D011092:Polyethylene Glycols; D048271:Chitosan; C005692:triphosphoric acid
keywords: Chitosan; Cytocompatibility; Electrospinning; Tripolyphosphate; Yield stress