title: Simultaneous detection of five major serotypes of Avian coronavirus by a multiplex microsphere-based assay.
abstract: Avian coronavirus (commonly known as Infectious bronchitis virus [IBV]) is of major economic importance to commercial chicken producers worldwide. Due to the existence of multiple serotypes and variants of the virus that do not cross-protect, it is important to diagnose circulating serotypes and choose the right vaccine type for successful protection. In an effort to improve conventional diagnostic tests, a microsphere-based assay was developed and evaluated for simultaneous detection of the most common IBV vaccine serotypes in the United States: Arkansas (Ark), Connecticut (Conn), Massachusetts (Mass), Delaware (DE072), and Georgia 98 (GA98). The analytical specificity and sensitivity, and diagnostic specificity and sensitivity, were evaluated. The microsphere-based assay was highly specific to designated serotypes and generated reproducible data. Comparing the microsphere-based assay to nucleotide sequencing, the 2 methods agreed more than 93% (kappa value > .77). In addition, the microsphere-based assay could detect coinfections in clinical samples. The results demonstrate the utility of the microsphere-based assay as a rapid and accurate diagnostic tool with the potential for high throughput diagnosis.
authors.affiliation: Department of Population Health, Poultry Diagnostic and Research Center, University of Georgia, Athens, GA 30602, USA.
authors.forename: Ha-Jung
authors.initials: HJ
authors.lastname: Roh
authors.forename: Deborah A
authors.initials: DA
authors.lastname: Hilt
authors.forename: Mark W
authors.initials: MW
authors.lastname: Jackwood
mesh_terms: D000818:Animals; D002645:Chickens; D018352:Coronavirus Infections / Q000175:diagnosis / Q000235:genetics / Q000662:veterinary* / Q000821:virology; D001351:Infectious bronchitis virus / Q000235:genetics / Q000302:isolation & purification*; D008863:Microspheres; D011201:Poultry Diseases / Q000175:diagnosis / Q000235:genetics / Q000821:virology*; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D012680:Sensitivity and Specificity; D012703:Serotyping / Q000379:methods / Q000662:veterinary*; D014481:United States
chemical_list: D012367:RNA, Viral
keywords: Arkansas (Ark); Avian coronavirus; Connecticut (Conn); Delaware 072 (DE072); Georgia 98 (GA98); Infectious bronchitis virus; Massachusetts (Mass); diagnosis; microsphere-based assay; multiplex; serotyping