title: Universal labeling of 5'-triphosphate RNAs by artificial RNA ligase enzyme with broad substrate specificity.
abstract: An artificial RNA ligase specific to RNA with a 5'-triphosphate (PPP-RNA) exhibits broad sequence specificity on model substrates and secondary siRNAs with direct applications in the identification of PPP-RNAs through sequencing.
authors.affiliation: Department of Biochemistry, Molecular Biology and Biophysics & BioTechnology Institute, University of Minnesota, 1479 Gortner Ave, Saint Paul, MN 55108, USA.
authors.forename: John C
authors.initials: JC
authors.lastname: Haugner
authors.forename: Burckhard
authors.initials: B
authors.lastname: Seelig
mesh_terms: D012313:RNA / Q000737:chemistry* / Q000235:genetics; D011130:RNA Ligase (ATP) / Q000737:chemistry* / Q000235:genetics; D034741:RNA, Small Interfering / Q000235:genetics; D017422:Sequence Analysis, DNA; D013379:Substrate Specificity
chemical_list: D034741:RNA, Small Interfering; D012313:RNA; D011130:RNA Ligase (ATP)