title: Expression of SGTA correlates with prognosis and tumor cell proliferation in human hepatocellular carcinoma.
abstract: To investigate the potential role of small glutamine-rich TPR-containing protein A (SGTA) in hepatocarcinogenesis, immunohistochemistry and Western blot were performed to detect the expression of SGTA in clinical Hepatocellular carcinoma (HCC) samples, adjacent nontumorous liver tissues and HCC cell lines. In addition, expression of SGTA was correlated with clinicopathological variables and univariate and multivariate survival analyses were performed to determine the prognostic significance. Moreover, the biological significance of the aberrant expression of SGTA was investigated in vitro. Both immunohistochemistry evaluation and Western blot analyses demonstrated that SGTA was overexpressed in HCC tissues compared with adjacent nontumorous liver tissues. Expression of SGTA directly correlated with the histological grades of HCC and high expression of SGTA was associated with a poor prognosis. SGTA depletion by siRNA inhibited cell proliferation, blocked S-phase and mitotic entry in Huh7 cells. Western blot analyses showed that SGTA depletion decreased cyclin A and cyclin B levels. Taken together, owing to overexpression of SGTA in HCC and its important role in predicting poor prognosis and the development of HCC, SGTA could be a potential prognostic marker and therapeutic target of HCC.
authors.affiliation: Department of Digestion, Affiliated Hospital of Nantong University, Medical College of Nantong University, Nantong, Jiangsu, 226001, People's Republic of China.
authors.forename: Cuihua
authors.initials: C
authors.lastname: Lu
authors.forename: Guoliang
authors.initials: G
authors.lastname: Liu
authors.forename: Xiaopeng
authors.initials: X
authors.lastname: Cui
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.forename: Lixian
authors.initials: L
authors.lastname: Wei
authors.forename: Yingying
authors.initials: Y
authors.lastname: Wang
authors.forename: Xiaojing
authors.initials: X
authors.lastname: Yang
authors.forename: Yanhua
authors.initials: Y
authors.lastname: Liu
authors.forename: Xia
authors.initials: X
authors.lastname: Cong
authors.forename: Liting
authors.initials: L
authors.lastname: Lv
authors.forename: Runzhou
authors.initials: R
authors.lastname: Ni
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Huang
mesh_terms: D000328:Adult; D000368:Aged; D006528:Carcinoma, Hepatocellular / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D002352:Carrier Proteins / Q000096:biosynthesis* / Q000235:genetics; D048708:Cell Growth Processes / Q000502:physiology; D045744:Cell Line, Tumor; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008113:Liver Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D018832:Molecular Chaperones; D011379:Prognosis
chemical_list: D002352:Carrier Proteins; D018832:Molecular Chaperones; C114575:SGTA protein, human