title: Detection of single-nucleotide variations by monitoring the blinking of fluorescence induced by charge transfer in DNA.
abstract: Charge transfer dynamics in DNA: Photo-induced charge separation and charge-recombination dynamics in DNA was assessed by monitoring the blinking of fluorescence. Single nucleotide variations, mismatch and one base deletion, were differentiated based on the length of the off-time of the blinking, which corresponds to the lifetime of the charge-separated state.
authors.affiliation: The Institute of Scientific and Industrial Research, Osaka University, Mihogaoka 8-1, Ibaraki, Japan. kiyohiko@sanken.osaka-u.ac.jp
authors.forename: Kiyohiko
authors.initials: K
authors.lastname: Kawai
authors.forename: Tetsuro
authors.initials: T
authors.lastname: Majima
authors.forename: Atsushi
authors.initials: A
authors.lastname: Maruyama
mesh_terms: D001483:Base Sequence; D004247:DNA / Q000235:genetics*; D006515:Hepatitis B virus / Q000737:chemistry / Q000235:genetics; D006576:Heterocyclic Compounds, 4 or More Rings / Q000737:chemistry*; D057230:Limit of Detection; D015335:Molecular Probes / Q000737:chemistry* / Q000235:genetics; D015394:Molecular Structure; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D013050:Spectrometry, Fluorescence
chemical_list: C585841:Atto655; D006576:Heterocyclic Compounds, 4 or More Rings; D015335:Molecular Probes; D004247:DNA
keywords: DNA; blinking; charge transfer; fluorescence correlation spectroscopy; genotyping