title: Biological therapy of strontium-substituted bioglass for soft tissue wound-healing: responses to oxidative stress in ovariectomised rats.
abstract: New synthetic biomaterials are constantly being developed for wound repair and regeneration. Bioactive glasses (BG) containing strontium have shown successful applications in tissue engineering account of their biocompatibility and the positive biological effects after implantation. This study aimed to assess whether BG-Sr was accepted by the host tissue and to characterize oxidative stress biomarker and antioxidant enzyme profiles during muscle and skin healing. Wistar rats were divided into five groups (six animals per group): the group (I) was used as negative control (T), after ovariectomy, groups II, III, IV and V were used respectively as positive control (OVX), implanted tissue with BG (OVX-BG), BG-Sr (OVX-BG-Sr) and presented empty defects (OVX-NI). Soft tissues surrounding biomaterials were used to estimate superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and malondialdehyde (MDA) concentration. Our results show that 60 days after operation, treatment of rats with BG-Sr significantly increased MDA concentration and caused an increase of SOD, CAT and GPx activities in both skin and muscular tissues. BG-Sr revealed maturation of myotubes followed a normal appearance of muscle regenerated with high density and mature capillary vessels. High wound recovery with complete re-epithelialization and regeneration of skin was observed. The results demonstrate that the protective action against reactive oxygen species (ROS) was clearly observed in soft tissue surrounding BG-Sr. Moreover, the potential use of BG-Sr rapidly restores the wound skin and muscle structural and functional properties. The BG advantages such as ion release might make BG-Sr an effective biomaterial choice for antioxidative activity.
authors.affiliation: University of Rennes-1, UMR CNRS 6226, campus de Beaulieu, 263, avenue du Général-Leclerc, 35042 Rennes, France. jbahisamira@yahoo.fr
authors.forename: S
authors.initials: S
authors.lastname: Jebahi
authors.forename: H
authors.initials: H
authors.lastname: Oudadesse
authors.forename: N
authors.initials: N
authors.lastname: Jardak
authors.forename: I
authors.initials: I
authors.lastname: Khayat
authors.forename: H
authors.initials: H
authors.lastname: Keskes
authors.forename: A
authors.initials: A
authors.lastname: Khabir
authors.forename: T
authors.initials: T
authors.lastname: Rebai
authors.forename: H
authors.initials: H
authors.lastname: El Feki
authors.forename: A
authors.initials: A
authors.lastname: El Feki
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000032:analysis; D001691:Biological Therapy / Q000379:methods*; D015415:Biomarkers; D002516:Ceramics / Q000737:chemistry / Q000627:therapeutic use*; D005260:Female; D018482:Muscle, Skeletal / Q000378:metabolism / Q000473:pathology; D010052:Ovariectomy; D018384:Oxidative Stress / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D012867:Skin / Q000378:metabolism / Q000473:pathology; D013324:Strontium / Q000737:chemistry / Q000627:therapeutic use*; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D000975:Antioxidants; C022107:Bioglass; D015415:Biomarkers; D013324:Strontium
keywords: Antioxidative activity; Bioglass; Des activités antioxydantes; La régénération des tissus mous; Le stress oxydatif; Oxidative stress; Soft tissue regeneration; Strontium; Verre bioactif