title: [The long-term dynamic changes of proliferation and differentiation of neural stem cells in subventricular zone and dentate gyrus of vascular dementia rats].
abstract: OBJECTIVE
  To observe the dynamic changes of proliferation and differentiation of neural stem cells (NSCs) in subventricular zone (SVZ) and dentate gyrus (DG) in vascular dementia (VD) rats.
  
  
  METHODS
  VD models were established by repeatedly clipping the common carotid arteries of the rat in combination with an intraperitoneal injection of sodium nitroprusside solution in anesthetized SD rats. Morris maze test was used to detect the learning and memory ability of the rats and immune fluorescence single and double labeling method to detect the proliferation and differentiation of neural stem cells and the expression of neurogranin (Ng) at 15 d, 1 month, 2 month, 4 month time points.
  
  
  RESULTS
  Compared with sham-operated group, the escape latency of model group rats were significantly longer at all the time points (P < 0.01). The BrdU positive cells in SVZ and DG of VD model groups were markedly increased in comparison with sham-operated group at 15 d and 1 month time point (P < 0.01), and the number of BrdU positive cells in SVZ of model groups were still larger than that of sham-operated group at 2 month and 4 month time point (P < 0.01). In model group, the number of the BrdU/Ng double staining cells were increased and higher than that in sham-operated groups (P < 0.05).
  
  
  CONCLUSIONS
  The proliferation of NSCs can be enhanced noticeably in a certain time in SVZ and DG region and NSCs differentiate into mature neurons with the expression of Ng in DG region in VD rats, which may play some compensatory roles in the nerve regeneration and functional repairmen after cerebral injury of VD.
authors.affiliation: School of Medicine, Shandong University, Jinan 250012, China.
authors.forename: Ji-Wei
authors.initials: JW
authors.lastname: Wang
authors.forename: Wen
authors.initials: W
authors.lastname: Chen
authors.forename: Yu-Liang
authors.initials: YL
authors.lastname: Wang
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D049109:Cell Proliferation; D015140:Dementia, Vascular; D018891:Dentate Gyrus / Q000166:cytology*; D004805:Ependyma / Q000166:cytology*; D008297:Male; D018782:Maze Learning; D009416:Nerve Regeneration; D058953:Neural Stem Cells / Q000166:cytology*; D051381:Rats; D017207:Rats, Sprague-Dawley