title: Millimeter-sized neural building blocks for 3D heterogeneous neural network assembly.
abstract: A millimeter-sized neural building block (NBB) shows high versatility to form a 3D heterogeneous neural component. A millimeter-sized 3D neural network between heterogeneous neural tissues is established, and an efficient technique is then developed to observe the spatiotemporal metrological changes of single neuron in the NBB. This technique allows the visualization of axonal extension, dendritic branching, and morphological changes of presynaptic components and synapses in real time.
authors.affiliation: Institute of Industrial Science, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8505, Japan; Takeuchi Biohybrid Innovation Project, Exploratory Research for Advanced Technology (ERATO), Japan Science and Technology Agency (JST), 4-6-1, Komaba, Meguro-ku, Tokyo 153-8505, Japan.
authors.forename: Midori
authors.initials: M
authors.lastname: Kato-Negishi
authors.forename: Yuya
authors.initials: Y
authors.lastname: Morimoto
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Onoe
authors.forename: Shoji
authors.initials: S
authors.lastname: Takeuchi
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology*; D006624:Hippocampus / Q000166:cytology*; D018613:Microscopy, Confocal; D008959:Models, Neurological; D009474:Neurons / Q000166:cytology* / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D018874:Spheroids, Cellular / Q000166:cytology; D023822:Tissue Engineering
keywords: assembly; cortex; hippocampus; neural network; three-dimensional neural tissue