title: On-line capillary electrophoresis enrichment by combining chitosan trapping with surfactant assisted sample stacking for the ultratrace determination of organic acids in Plateau alfalfa roots.
abstract: In this paper, four organic acids constituents of Plateau alfalfa roots have been identified and detected by a novel capillary electrophoresis (CE) strategy which combined chitosan (CS) trapping and cetyltrimethyl ammonium bromide (CTAB) assisted sample stacking. Under the optimized condition, organic acids, i.e., aconitic acid, gallic acid, citric acid and l-malic acid were concentrated and separated within 3 min. Validation parameters of this method (such as detection limits, linearity and precision) were also investigated and the limit of detection (LOD) was 2.41-53.9 ng mL(-1). Linearity was obtained over the magnitude range of 5-4000 ng mL(-1) approximately for different organic acids and 3×10(2)-1.5×10(4) folds enrichment was achieved. The method has been applied to the determination of organic acids in roots of normal grown Plateau alfalfa and stressing affected Plateau alfalfa. Satisfactory results and recoveries were obtained in the analysis without costly and complicated sample pretreatment.
authors.affiliation: State Key Laboratory of Applied Organic Chemistry, Lanzhou University, Lanzhou 730000, China.
authors.forename: Xi
authors.initials: X
authors.lastname: Li
authors.forename: Yuyun
authors.initials: Y
authors.lastname: Ju
authors.forename: Yinyin
authors.initials: Y
authors.lastname: Xu
authors.forename: Weifeng
authors.initials: W
authors.lastname: Wang
authors.forename: Yalei
authors.initials: Y
authors.lastname: Dong
authors.forename: Yanhua
authors.initials: Y
authors.lastname: Ma
authors.forename: Xingguo
authors.initials: X
authors.lastname: Chen
mesh_terms: D002264:Carboxylic Acids / Q000032:analysis*; D048271:Chitosan / Q000737:chemistry; D019343:Citric Acid / Q000032:analysis; D019075:Electrophoresis, Capillary / Q000379:methods*; D057230:Limit of Detection; D000455:Medicago sativa / Q000737:chemistry*; D009862:Online Systems; D009930:Organic Chemicals / Q000032:analysis; D018517:Plant Roots / Q000737:chemistry*; D015203:Reproducibility of Results; D013501:Surface-Active Agents
chemical_list: D002264:Carboxylic Acids; D009930:Organic Chemicals; D013501:Surface-Active Agents; D019343:Citric Acid; D048271:Chitosan
keywords: Capillary electrophoresis; On-line concentration; Organic acids; Plateau alfalfa roots