title: ROQUIN/RC3H1 alterations are not found in angioimmunoblastic T-cell lymphoma.
abstract: Angioimmunoblastic T-cell Lymphoma (AITL) is one of the most frequent T-cell lymphoma entities. Follicular helper T lymphocytes (TFH) are recognized as the normal cellular counterpart of the neoplastic component. Despite a clonal T-cell feature and few described recurrent cytogenetic abnormalities, a driving oncogenic event has not been identified so far. It has been recently reported that in mice, heterozygous inactivation of Roquin/Rc3h1, a RING type E3 ubiquitine ligase, recapitulates many of the clinical, histological, and cellular features associated with human AITL. In this study we explored whether ROQUIN alterations could be an initial event in the human AITL oncogenic process. Using microarray and RT-PCR analyses, we investigated the levels of ROQUIN transcripts in TFH tumor cells purified from AITL (n = 8) and reactive tonsils (n = 12) and found similar levels of ROQUIN expression in both. Moreover, we also demonstrated that ROQUIN protein was expressed by AITL TFH (PD1+) cells. We then analysed ROQUIN coding sequence in 12 tumor cell-rich AITL samples and found no mutation in any of the samples. Finally, we analysed the expression of MiR101, a putative partner of ROQUIN involved in the modulation of ICOS expression and found similar levels of expression in tumor and reactive TFH. Altogether, this study shows that neither alteration of ROQUIN gene nor deregulation of miR101 expression is likely to be a frequent recurrent event in AITL.
authors.affiliation: INSERM U955, Immunologie et Oncogenèse des Tumeurs Lymphoïdes, Créteil, France.
authors.forename: Tiphanie
authors.initials: T
authors.lastname: Auguste
authors.forename: Marion
authors.initials: M
authors.lastname: Travert
authors.forename: Karin
authors.initials: K
authors.lastname: Tarte
authors.forename: Patricia
authors.initials: P
authors.lastname: Amé-Thomas
authors.forename: Catherine
authors.initials: C
authors.lastname: Artchounin
authors.forename: Nadine
authors.initials: N
authors.lastname: Martin-Garcia
authors.forename: Aurélien
authors.initials: A
authors.lastname: de Reynies
authors.forename: Laurence
authors.initials: L
authors.lastname: de Leval
authors.forename: Philippe
authors.initials: P
authors.lastname: Gaulard
authors.forename: Marie-Hélène
authors.initials: MH
authors.lastname: Delfau-Larue
mesh_terms: D002478:Cells, Cultured; D006801:Humans; D007119:Immunoblastic Lymphadenopathy / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D007150:Immunohistochemistry; D016399:Lymphoma, T-Cell / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D044767:Ubiquitin-Protein Ligases / Q000235:genetics / Q000378:metabolism*
chemical_list: C501406:Rc3h1 protein, mouse; D044767:Ubiquitin-Protein Ligases