title: Hydrogen sulfide induces heme oxygenase-1 in human kidney cells.
abstract: Hydrogen sulfide (H2S) has been shown to have a potential protective role in a number of disease states including diabetes and various kidney disorders; however, the mechanisms involved are still unclear. The aim of this study was to investigate if H2S effects the expression of the antioxidant enzyme heme oxygenase-1 (HO-1) in human kidney cells. Human mesangial cells and human podocytes were cultured at normal physiological glucose concentration (5.5 mM) and then treated with different H2S donors for a 24-h period. Protein was then extracted from the cells, and the expression levels of HO-1 determined by Western blotting. There was a significant increase in HO-1 expression after treatment with the H2S donors in both mesangial and podocyte cells. These results suggest that H2S has a role in the regulation of HO-1 expression, and the ability to upregulate this antioxidant enzyme maybe a potential mechanism by which H2S exerts its protective effects.
authors.affiliation: Department of Molecular Medicine, Institute of Translational and Stratified Medicine, Plymouth University Peninsula Schools of Medicine and Dentistry, John Bull Building, Research Way, Plymouth, PL6 8BU, England, UK.
authors.forename: Eliana
authors.initials: E
authors.lastname: D'Araio
authors.forename: Nicholas
authors.initials: N
authors.lastname: Shaw
authors.forename: Ann
authors.initials: A
authors.lastname: Millward
authors.forename: Andrew
authors.initials: A
authors.lastname: Demaine
authors.forename: Matt
authors.initials: M
authors.lastname: Whiteman
authors.forename: Andrea
authors.initials: A
authors.lastname: Hodgkinson
mesh_terms: D000975:Antioxidants / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004790:Enzyme Induction / Q000187:drug effects; D051547:Heme Oxygenase-1 / Q000096:biosynthesis*; D006801:Humans; D006862:Hydrogen Sulfide / Q000494:pharmacology*; D007668:Kidney / Q000187:drug effects* / Q000378:metabolism*; D050527:Mesangial Cells / Q000187:drug effects / Q000378:metabolism; D050199:Podocytes / Q000187:drug effects / Q000378:metabolism
chemical_list: D000975:Antioxidants; D051547:Heme Oxygenase-1; D006862:Hydrogen Sulfide