title: Comparison of the sequestering properties of yeast cell wall extract and hydrated sodium calcium aluminosilicate in three in vitro models accounting for the animal physiological bioavailability of zearalenone.
abstract: The sequestration/inactivation of the oestrogenic mycotoxin zearalenone (ZEA) by two adsorbents--yeast cell wall extract (YCW) and hydrated sodium calcium aluminosilicate (HSCAS)--was studied in three laboratory models: (1) an in vitro model was adapted from referenced methods to test for the sequestrant sorption capabilities under buffer conditions at two pH values using liquid chromatography coupled to a fluorescence detector for toxin quantification; (2) a second in vitro model was used to evaluate the sequestrant sorption stability according to pH variations and using ³H-labelled ZEA at low toxin concentration; and (3) an original, ex vivo Ussing chamber model was developed to further understand the transfer of ZEA through intestinal tissue and the impact of each sequestrant on the mycotoxin bioavailability of ³H-labelled ZEA. YCW was a more efficient ZEA adsorbent than HSCAS in all three models, except under very acidic conditions (pH 2.5 or 3.0). The Ussing chamber model offered a novel, ex vivo, alternative method for understanding the effect of sequestrant on the bioavailability of ZEA. The results showed that compared with HSCAS, YCW was more efficient in sequestering ZEA and that it reduced the accumulation of ZEA in the intestinal tissue by 40% (p < 0.001).
authors.affiliation: a Alltech, Inc. , Nicholasville , KY , USA.
authors.forename: A
authors.initials: A
authors.lastname: Yiannikouris
authors.forename: H
authors.initials: H
authors.lastname: Kettunen
authors.forename: J
authors.initials: J
authors.lastname: Apajalahti
authors.forename: E
authors.initials: E
authors.lastname: Pennala
authors.forename: C A
authors.initials: CA
authors.lastname: Moran
mesh_terms: D000538:Aluminum Silicates / Q000737:chemistry / Q000378:metabolism; D000821:Animal Feed; D000818:Animals; D001688:Biological Products / Q000737:chemistry / Q000378:metabolism*; D002473:Cell Wall / Q000737:chemistry*; D011232:Chemical Precipitation; D004968:Estrogens, Non-Steroidal / Q000037:antagonists & inhibitors* / Q000737:chemistry / Q000378:metabolism; D005503:Food Additives / Q000737:chemistry / Q000378:metabolism; D005765:Gastrointestinal Agents / Q000737:chemistry / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007082:Ileum / Q000378:metabolism; D066298:In Vitro Techniques; D007408:Intestinal Absorption; D007413:Intestinal Mucosa / Q000378:metabolism; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012441:Saccharomyces cerevisiae / Q000737:chemistry*; D064449:Sequestering Agents / Q000737:chemistry / Q000378:metabolism*; D012995:Solubility; D015025:Zearalenone / Q000037:antagonists & inhibitors* / Q000737:chemistry / Q000378:metabolism
chemical_list: D000538:Aluminum Silicates; D001688:Biological Products; D004968:Estrogens, Non-Steroidal; D005503:Food Additives; D005765:Gastrointestinal Agents; D064449:Sequestering Agents; C087965:sodium calcium aluminosilicate, hydrated; D015025:Zearalenone