title: Real-time diagnosis of chemically induced hepatocellular carcinoma using a novel mass spectrometry-based technique.
abstract: Real-time analyses of hepatocellular carcinoma were performed in living mice to assess the applicability of probe electrospray ionization-mass spectrometry (PESI-MS) in medical diagnosis. The number of peaks and the abundance of ions corresponding to triacylglycerols (TAGs) were higher in cancerous tissues than in noncancerous tissues. Multiple sequential scans of the specimens were performed along a predetermined line extending over the noncancerous region to detect the boundary of the cancerous region. Our system successfully discriminated the noncancerous and cancerous tissues based on the intensities of the TAG ions. These results highlight the potential application of PESI-MS for clinical diagnosis in cancer.
authors.affiliation: Department of Anatomy and Cell Biology, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, Chuo, Yamanashi 409-3898, Japan.
authors.forename: Kentaro
authors.initials: K
authors.lastname: Yoshimura
authors.forename: Mridul Kanti
authors.initials: MK
authors.lastname: Mandal
authors.forename: Michio
authors.initials: M
authors.lastname: Hara
authors.forename: Hideki
authors.initials: H
authors.lastname: Fujii
authors.forename: Lee Chuin
authors.initials: LC
authors.lastname: Chen
authors.forename: Kunio
authors.initials: K
authors.lastname: Tanabe
authors.forename: Kenzo
authors.initials: K
authors.lastname: Hiraoka
authors.forename: Sen
authors.initials: S
authors.lastname: Takeda
mesh_terms: D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000139:chemically induced / Q000175:diagnosis*; D004052:Diethylnitrosamine; D006801:Humans; D008113:Liver Neoplasms / Q000139:chemically induced / Q000175:diagnosis*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009374:Neoplasms, Experimental / Q000139:chemically induced / Q000175:diagnosis*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D013997:Time Factors
chemical_list: D004052:Diethylnitrosamine
keywords: Cancer diagnosis; Electrospray ionization; Hepatocellular carcinoma; Lipid; Mass spectrometry