title: Efficacy of Clostridium botulinum types C and D toxoid vaccination in Danish cows.
abstract: In the present study the efficacy Botulism vaccine (formalinised aluminum hydroxide gel adsorbed toxoid of Clostridium botulinum types C and D) was evaluated in four Danish dairy cows under field conditions. Other four dairy herds were unvaccinated. Blood serum of all animals was analyzed for specific C. botulinum types A, B, C, D and E antibodies using a developed ELISA. Feces of all animals were analyzed for botulinum neurotoxins (BoNTs) and C. botulinum spores. C. botulinum types C and D antibodies were significantly (p < 0.05) increased in vaccinated animals. Vaccination with botulism vaccine significantly reduced (p < 0.001) BoNTs and C. botulinum spores in cattle feces. Our findings represent that C. botulinum vaccination increases specific blood serum antibodies and reduces free BoNTs and C. botulinum spores in feces.
authors.affiliation: Institute of Bacteriology and Mycology, Faculty of Veterinary Medicine, University of Leipzig, An den Tierkliniken 29, D-04103 Leipzig, Germany.
authors.forename: Monika
authors.initials: M
authors.lastname: Krüger
authors.forename: Marie
authors.initials: M
authors.lastname: Skau
authors.forename: Awad Ali
authors.initials: AA
authors.lastname: Shehata
authors.forename: Wieland
authors.initials: W
authors.lastname: Schrödl
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D000992:Antitoxins / Q000097:blood; D058491:Bacterial Load; D001428:Bacterial Vaccines / Q000008:administration & dosage / Q000276:immunology*; D001905:Botulinum Toxins / Q000032:analysis / Q000276:immunology*; D001906:Botulism / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary*; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology / Q000517:prevention & control*; D003014:Clostridium botulinum / Q000302:isolation & purification; D003718:Denmark; D004797:Enzyme-Linked Immunosorbent Assay; D005243:Feces / Q000737:chemistry / Q000382:microbiology; D013171:Spores, Bacterial / Q000302:isolation & purification; D014121:Toxoids / Q000008:administration & dosage / Q000276:immunology*; D014611:Vaccination / Q000379:methods*
chemical_list: D000907:Antibodies, Bacterial; D000992:Antitoxins; D001428:Bacterial Vaccines; D014121:Toxoids; D001905:Botulinum Toxins
keywords: Botulinum neurotoxins; Botulism; Clostridium botulinum; ELISA; Vaccination