title: The performance of an orthosilicic acid-releasing silica gel fiber fleece in wound healing.
abstract: In the present work, we have examined the impact of an inorganic orthosilicic acid-releasing spun fiber fleece (SIFIB) on wound closure in a porcine wound model in vivo as well as on wound healing-relevant parameters in vitro. In vivo SIFIB was completely bio-degradable and had no negative effects on wound closure or the wound healing process. In the in vitro experiments, SIFIB had no negative effects on proliferation of human skin fibroblast (FB) and endothelial cell (EC) cultures but strongly retarded the growth of the human monocyte cell line THP-1, and effectively inhibited human skin keratinocyte (KC) proliferation, which based on significantly enhanced KC differentiation. Furthermore, SIFIB exhibited strong anti-inflammatory properties, which based on SIFIB-dependent inhibition of expression and activity of NF-кB and/or concomitant enhanced expression of IкB, a NF-кB-inhibiting protein. Additionally, SIFIB significantly inhibited TGFβ-induced fibroblast differentiation and collagen synthesis as well as effectively reduced TGF-β synthesis of activated fibroblasts. We have demonstrated wound healing-relevant biological activities of a silica-based bio-degradable inorganic material, which might represent a new therapeutic tool in the treatment of chronic wounds.
authors.affiliation: Department of Trauma and Hand Surgery, Medical Faculty of the Heinrich-Heine-University Düsseldorf, Düsseldorf, Germany.
authors.forename: Vera
authors.initials: V
authors.lastname: Grotheer
authors.forename: Maria
authors.initials: M
authors.lastname: Goergens
authors.forename: Paul C
authors.initials: PC
authors.lastname: Fuchs
authors.forename: Sebastian
authors.initials: S
authors.lastname: Dunda
authors.forename: Norbert
authors.initials: N
authors.lastname: Pallua
authors.forename: Joachim
authors.initials: J
authors.lastname: Windolf
authors.forename: Christoph V
authors.initials: CV
authors.lastname: Suschek
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology; D015153:Blotting, Western; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D003094:Collagen / Q000378:metabolism; D016207:Cytokines / Q000378:metabolism; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D018836:Inflammation Mediators / Q000378:metabolism; D015603:Keratinocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D008297:Male; D016328:NF-kappa B / Q000378:metabolism; D017646:Organosilicon Compounds / Q000494:pharmacology*; D058428:Silica Gel / Q000494:pharmacology*; D012824:Silicic Acid / Q000494:pharmacology*; D013054:Spectrophotometry, Atomic; D013552:Swine; D013556:Swine, Miniature; D016212:Transforming Growth Factor beta / Q000378:metabolism; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D001672:Biocompatible Materials; D016207:Cytokines; D018836:Inflammation Mediators; D016328:NF-kappa B; D017646:Organosilicon Compounds; D016212:Transforming Growth Factor beta; D012824:Silicic Acid; D058428:Silica Gel; D003094:Collagen
keywords: IL-1β; IL-6; IL-8; Inflammation; NF-кB; Silica