title: Instantaneous three-dimensional sensing using spatial light modulator illumination with extended depth of field imaging.
abstract: Imaging three-dimensional structures represents a major challenge for conventional microscopies. Here we describe a Spatial Light Modulator (SLM) microscope that can simultaneously address and image multiple targets in three dimensions. A wavefront coding element and computational image processing enables extended depth-of-field imaging. High-resolution, multi-site three-dimensional targeting and sensing is demonstrated in both transparent and scattering media over a depth range of 300-1,000 microns.
authors.affiliation: Howard Hughes Medical Institute, Department of Biological Sciences, Columbia University, 550 West 122nd Street, New York, NY 10027, USA. sq2142@columbia.edu
authors.forename: Sean
authors.initials: S
authors.lastname: Quirin
authors.forename: Darcy S
authors.initials: DS
authors.lastname: Peterka
authors.forename: Rafael
authors.initials: R
authors.lastname: Yuste