title: A SEM and non-contact surface white light profilometry in vivo study of the effect of a crème containing CPP-ACP and fluoride on young etched enamel.
abstract: The aim of this in vivo study was to evaluate the short and a longer term effect on enamel of the application of a crème containing 10% CPP-ACP and 900 ppm fluoride, in orthodontically planned, high caries-risk patients. Epoxy resin replicas of upper lateral incisors were obtained from polyvinyl siloxane (PVS) impressions, before and after etching. The right incisors were left untreated in order to control saliva remineralizing potential. The upper left surfaces were coated with a pea-size amount of the crème. Replicas were obtained at 3 weeks and 6 months and analyzed by SEM and non-contact surface white light profilometry. In the treated sample the profilometric roughness parameters at 3 weeks were statistically significantly lower than the control group values (p < 0.05). At 3 weeks SEM images of the enamel surface showed fewer irregularities. After 6 months, differences between test and control groups were not present on SEM images and profilometric values. CPP-ACP and fluoride crème had positive in vivo effects on enamel surfaces. Significant differences in surface roughness existed after a 3-week period of crème use.
authors.affiliation: DIBINEM, Section of Dental Sciences, University of Bologna, Bologna, Italy.
authors.forename: Chiara
authors.initials: C
authors.lastname: Baroni
authors.affiliation: DIBINEM, Section of Dental Sciences, University of Bologna, Bologna, Italy.
authors.forename: Silvia
authors.initials: S
authors.lastname: Marchionni
authors.affiliation: DIBINEM, Section of Dental Sciences, University of Bologna, Bologna, Italy.
authors.forename: Maria Giulia
authors.initials: MG
authors.lastname: Bazzocchi
authors.affiliation: Department of Biomedicine, University of Trieste, Trieste, Italy.
authors.forename: Milena
authors.initials: M
authors.lastname: Cadenaro
authors.affiliation: DIBINEM, Section of Dental Sciences, University of Bologna, Bologna, Italy.
authors.forename: Cesare
authors.initials: C
authors.lastname: Nucci
authors.affiliation: Melbourne Dental School, University of Melbourne, Melbourne, Victoria, Australia.
authors.forename: David J
authors.initials: DJ
authors.lastname: Manton
mesh_terms: D002364:Caseins / Q000378:metabolism*; D003743:Dental Enamel / Q000187:drug effects* / Q000648:ultrastructure; D005459:Fluorides / Q000378:metabolism*; D006801:Humans; D007180:Incisor / Q000187:drug effects / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning; D013499:Surface Properties / Q000187:drug effects*; D016896:Treatment Outcome
chemical_list: D002364:Caseins; C447032:casein phosphopeptide-amorphous calcium phosphate nanocomplex; D005459:Fluorides
keywords: SEM; dentistry; microstructure; replicas; surface analisys