title: Sensitive determination of 2-methoxyestradiol in pharmaceutical preparations and serum samples using flow injection chemiluminescence.
abstract: A rapid and sensitive flow injection chemiluminescence (FI-CL) method is described for the determination of 2-methoxyestradiol (2ME) based on enhancement of the CL intensity from a potassium ferricyanide-calcein system in sodium hydroxide medium. The optimum conditions for the CL emission were investigated. Under optimized conditions, a linear calibration graph was obtained over the range 1.0 × 10(-8) to 1.0 × 10(-6) mol/L (r = 0.998) 2ME with a detection limit (3σ) of 5.4 × 10(-9) mol/L. The relative standard deviation (RSD) for 5.0 × 10(-7) mol/L 2ME was 1.7%. As a preliminary application, the proposed method was successfully applied to the determination of 2ME in injection solutions and serum samples. The possible CL mechanism was also proposed.
authors.affiliation: School of Pharmaceutical Science, Zhengzhou University, Zhengzhou, China.
authors.forename: Hanchun
authors.initials: H
authors.lastname: Yao
authors.forename: Min
authors.initials: M
authors.lastname: Zhang
authors.forename: Wenyuan
authors.initials: W
authors.lastname: Zeng
authors.forename: Xiaoying
authors.initials: X
authors.lastname: Zeng
authors.forename: Zhenzhong
authors.initials: Z
authors.lastname: Zhang
mesh_terms: D000077584:2-Methoxyestradiol; D004958:Estradiol / Q000031:analogs & derivatives* / Q000032:analysis / Q000097:blood; D017022:Flow Injection Analysis / Q000379:methods*; D006801:Humans; D057230:Limit of Detection; D008163:Luminescent Measurements / Q000379:methods*; D004364:Pharmaceutical Preparations / Q000032:analysis*
chemical_list: D004364:Pharmaceutical Preparations; D004958:Estradiol; D000077584:2-Methoxyestradiol
keywords: 2-methoxyestradiol; calcein; chemiluminescence; potassium ferricyanide