title: Crystal structure of a member of a novel family of dioxygenases (PF10014) reveals a conserved cupin fold and active site.
abstract: PF10014 is a novel family of 2-oxyglutarate-Fe(2+) -dependent dioxygenases that are involved in biosynthesis of antibiotics and regulation of biofilm formation, likely by catalyzing hydroxylation of free amino acids or other related ligands. The crystal structure of a PF10014 member from Methylibium petroleiphilum at 1.9 Å resolution shows strong structural similarity to cupin dioxygenases in overall fold and active site, despite very remote homology. However, one of the β-strands of the cupin catalytic core is replaced by a loop that displays conformational isomerism that likely regulates the active site.
authors.affiliation: Joint Center for Structural Genomics, La Jolla, California (http://www.jcsg.org); Stanford Synchrotron Radiation Lightsource, SLAC National Accelerator Laboratory, Menlo Park, California, 94025.
authors.forename: Qingping
authors.initials: Q
authors.lastname: Xu
authors.forename: Joanna
authors.initials: J
authors.lastname: Grant
authors.forename: Hsiu-Ju
authors.initials: HJ
authors.lastname: Chiu
authors.forename: Carol L
authors.initials: CL
authors.lastname: Farr
authors.forename: Lukasz
authors.initials: L
authors.lastname: Jaroszewski
authors.forename: Mark W
authors.initials: MW
authors.lastname: Knuth
authors.forename: Mitchell D
authors.initials: MD
authors.lastname: Miller
authors.forename: Scott A
authors.initials: SA
authors.lastname: Lesley
authors.forename: Adam
authors.initials: A
authors.lastname: Godzik
authors.forename: Marc-André
authors.initials: MA
authors.lastname: Elsliger
authors.forename: Ashley M
authors.initials: AM
authors.lastname: Deacon
authors.forename: Ian A
authors.initials: IA
authors.lastname: Wilson
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D020134:Catalytic Domain / Q000235:genetics*; D003001:Cloning, Molecular; D042621:Comamonadaceae / Q000201:enzymology*; D017124:Conserved Sequence / Q000235:genetics*; D003460:Crystallization; D017931:DNA Primers / Q000235:genetics; D049308:Dioxygenases / Q000737:chemistry* / Q000235:genetics; D008958:Models, Molecular; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA
chemical_list: D017931:DNA Primers; D049308:Dioxygenases
keywords: 2-oxyglutarate; PF10014/BsmA; cupin dioxygenase; ferrous iron; free amino acids