title: Kinetic resolution and stereoselective synthesis of 3-substituted aspartic acids by using engineered methylaspartate ammonia lyases.
abstract: Enzymatic amino acid synthesis: Kinetic resolution and asymmetric synthesis of various valuable 3-substituted aspartic acids, which were obtained in fair to good yields with diastereomeric ratio values of up to >98:2 and enantiomeric excess values of up to >99 %, by using engineered methylaspartate ammonia lyases are described. These biocatalytic methodologies for the selective preparation of aspartic acid derivatives appear to be attractive alternatives for existing chemical methods.
authors.affiliation: Department of Pharmaceutical Biology, Groningen Research Institute of Pharmacy, University of Groningen, Antonius Deusinglaan 1, 9713 AV Groningen, The Netherlands.
authors.forename: Hans
authors.initials: H
authors.lastname: Raj
authors.forename: Wiktor
authors.initials: W
authors.lastname: Szymanski
authors.forename: Jandré
authors.initials: J
authors.lastname: de Villiers
authors.forename: Vinod
authors.initials: V
authors.lastname: Puthan Veetil
authors.forename: Wim J
authors.initials: WJ
authors.lastname: Quax
authors.forename: Keiko
authors.initials: K
authors.lastname: Shimamoto
authors.forename: Dick B
authors.initials: DB
authors.lastname: Janssen
authors.forename: Ben L
authors.initials: BL
authors.lastname: Feringa
authors.forename: Gerrit J
authors.initials: GJ
authors.lastname: Poelarends
mesh_terms: D000586:Amination; D019943:Amino Acid Substitution; D000642:Ammonia-Lyases / Q000235:genetics / Q000378:metabolism*; D001224:Aspartic Acid / Q000096:biosynthesis* / Q000737:chemistry; D055162:Biocatalysis; D007700:Kinetics; D013237:Stereoisomerism
chemical_list: D001224:Aspartic Acid; D000642:Ammonia-Lyases; C034881:methylaspartate ammonia-lyase
keywords: aspartic acid; asymmetric synthesis; biocatalysis; kinetic resolution; methylaspartate ammonia lyase