title: Microsecond dynamics of an unfolded protein by a line confocal tracking of single molecule fluorescence.
abstract: We present a new method for high speed tracking of fluorescence time series from single proteins. The method uses a fast sample flow and a modified confocal microscopy, line confocal microscopy, and achieves the time resolution of less than 20 μs. The obtained time series from the B domain of protein A labeled with donor and acceptor fluorophores suggest conformational heterogeneity and dynamic fluctuations in the unfolded state.
authors.affiliation: Institute of Multidisciplinary Research for Advanced Materials, Tohoku University, 2-1-1 Katahira, Aoba, Sendai, Miyagi 980-8577, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Oikawa
authors.forename: Yuta
authors.initials: Y
authors.lastname: Suzuki
authors.forename: Masataka
authors.initials: M
authors.lastname: Saito
authors.forename: Kiyoto
authors.initials: K
authors.lastname: Kamagata
authors.forename: Munehito
authors.initials: M
authors.lastname: Arai
authors.forename: Satoshi
authors.initials: S
authors.lastname: Takahashi
mesh_terms: D018613:Microscopy, Confocal / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000379:methods*; D011489:Protein Denaturation; D011506:Proteins / Q000737:chemistry*
chemical_list: D011506:Proteins