title: [Effects of qingre xiaoji recipe on the migration, chemotaxis, and tube formation capability of human lung adenocarcinoma cell induced human umbilical vein endothelial cells].
abstract: OBJECTIVE
  To study the effects of Qingre Xiaoji Recipe (QXR) on the migration, chemotaxis, and tube formation capability of human umbilical vein endothelial cells (HUVECs) induced by human lung adenocarcinoma cells (SPC-A-1).
  
  
  METHODS
  The QXR groups at different final concentrations (2.5, 5.0, 10.0, and 20.0 mg/mL) and a control group were set up. Then the chemotaxis and tube formation capabilities were observed using Erasion trace test, Transwell chamber assay, and Matrigel matrix tube formation assay.
  
  
  RESULTS
  QXR at three concentrations (2.5, 5.0, and 10.0 mg/mL) had inhibition on the migration of HUVECs cultured by SPC-A-1 cell supernatant (P<0.05). Besides, the migration rate decreased along with increased concentrations. The number of chemotactic HUVECs was significantly less than that of the positive control group (P <0.01), and the inhibition rate of chemokine increased along with increased concentrations. Furthermore, the number of chemotactic HUVECs was negatively correlated to the drug concentration (r = -0.830, P <0.01). The area of HUVEC cell tubule formation was significantly lower than that of the positive control group (P <0.01). The inhibition rate of HUVEC cell tubule formation increased along with increased drug concentration. Besides, the area of HUVEC cell tubular formation was negatively correlated with the drug concentration (r = -0.937, P <0.01).
  
  
  CONCLUSIONS
  The mechanism of QXR for fighting against tumor angiogenesis might be correlated with its inhibition on the migration, chemotaxis, and tube formation of tumor vascular endothelial cells.
authors.affiliation: Department of Oncology, The First Hospital Affiliated to Zhejiang University of Traditional Chinese Medicine, Hangzhou, China. CPF12345@163.com
authors.forename: Pei-feng
authors.initials: PF
authors.lastname: Chen
authors.forename: Lei
authors.initials: L
authors.lastname: Pan
authors.forename: Yin-qi
authors.initials: YQ
authors.lastname: Jin
mesh_terms: D000230:Adenocarcinoma / Q000098:blood supply / Q000473:pathology; D000077192:Adenocarcinoma of Lung; D045744:Cell Line, Tumor; D002633:Chemotaxis / Q000187:drug effects*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D061307:Human Umbilical Vein Endothelial Cells / Q000166:cytology / Q000187:drug effects*; D006801:Humans; D008175:Lung Neoplasms / Q000098:blood supply / Q000473:pathology; D009389:Neovascularization, Pathologic
chemical_list: D004365:Drugs, Chinese Herbal