title: DdrA, DdrD, and PprA: components of UV and mitomycin C resistance in Deinococcus radiodurans R1.
abstract: Mutants created by deleting the ddrA, ddrB, ddrC, ddrD, and pprA loci of Deinococcus radiodurans R1alone and in all possible combinations of pairs revealed that the encoded gene products contribute to this species' resistance to UV light and/or mitomycin C. Deleting pprA from an otherwise wild type cell sensitizes the resulting strain to UV irradiation, reducing viability by as much as eight fold relative to R1. If this deletion is introduced into a ΔddrA or ΔddrD background, the resulting strains become profoundly sensitive to the lethal effects of UV light. At a fluence of 1000 Jm⁻², the ΔddrA ΔpprA and ΔddrD ΔpprA strains are 100- and 1000-fold more sensitive to UV relative to the strain that has only lost pprA. Deletion of ddrA results in a 100 fold increase in strain sensitivity to mitomycin C, but in backgrounds that combine a deletion of ddrA with deletions of either ddrC or ddrD, mitomycin resistance is restored to wild type levels. Inactivation of ddrB also increases D. radiodurans sensitivity to mitomycin, but unlike the ddrA mutant deleting ddrC or ddrD from a ΔddrB background further increases that sensitivity. Despite the effect that loss of these gene products has on DNA damage resistance, none appear to directly affect either excision repair or homologous recombination suggesting that they participate in novel processes that facilitate tolerance to UV light and interstrand crosslinks in this species.
authors.affiliation: Department of Biological Sciences, Louisiana State University and A & M College, Baton Rouge, Louisiana, United States of America.
authors.forename: Kathiresan
authors.initials: K
authors.lastname: Selvam
authors.forename: Jana R
authors.initials: JR
authors.lastname: Duncan
authors.forename: Masashi
authors.initials: M
authors.lastname: Tanaka
authors.forename: John R
authors.initials: JR
authors.lastname: Battista
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000235:genetics*; D034301:Deinococcus / Q000166:cytology / Q000187:drug effects* / Q000235:genetics / Q000528:radiation effects*; D017353:Gene Deletion; D005798:Genes, Bacterial; D050296:Microbial Viability / Q000187:drug effects / Q000528:radiation effects; D016685:Mitomycin / Q000494:pharmacology*; D014466:Ultraviolet Rays
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D016685:Mitomycin