title: Modulation of post-antibiotic bacterial community reassembly and host response by Candida albicans.
abstract: The introduction of Candida albicans into cefoperazone-treated mice results in changes in bacterial community reassembly. Our objective was to use high-throughput sequencing to characterize at much greater depth the specific changes in the bacterial microbiome. The colonization of C. albicans significantly altered bacterial community reassembly that was evident at multiple taxonomic levels of resolution. There were marked changes in the levels of Bacteriodetes and Lactobacillaceae. Lachnospiraceae and Ruminococcaceae, the two most abundant bacterial families, did not change in relative proportions after antibiotics, but there were marked genera-level shifts within these two bacterial families. The microbiome shifts occurred in the absence of overt intestinal inflammation. Overall, these experiments demonstrate that the introduction of a single new microbe in numerically inferior numbers into the bacterial microbiome during a broad community disturbance has the potential to significantly alter the subsequent reassembly of the bacterial community as it recovers from that disturbance.
authors.affiliation: Division of Pulmonary and Critical Care Medicine, Department of Internal Medicine, University of Michigan Medical School, USA.
authors.forename: John R
authors.initials: JR
authors.lastname: Erb Downward
authors.forename: Nicole R
authors.initials: NR
authors.lastname: Falkowski
authors.forename: Katie L
authors.initials: KL
authors.lastname: Mason
authors.forename: Ryan
authors.initials: R
authors.lastname: Muraglia
authors.forename: Gary B
authors.initials: GB
authors.lastname: Huffnagle
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001419:Bacteria / Q000145:classification / Q000187:drug effects / Q000235:genetics; D044822:Biodiversity; D002176:Candida albicans / Q000502:physiology*; D002432:Cecum / Q000276:immunology / Q000378:metabolism / Q000382:microbiology / Q000473:pathology; D016000:Cluster Analysis; D005260:Female; D020869:Gene Expression Profiling; D054884:Host-Pathogen Interactions / Q000187:drug effects; D007082:Ileum / Q000276:immunology / Q000378:metabolism / Q000382:microbiology / Q000473:pathology; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000276:immunology / Q000382:microbiology* / Q000473:pathology; D051379:Mice; D064307:Microbiota; D013559:Symbiosis / Q000187:drug effects
chemical_list: D000900:Anti-Bacterial Agents