title: Effects of estrogen receptor α and β on the expression of visfatin and retinol-binding protein 4 in 3T3-L1 adipocytes.
abstract: The aim of this study was to investigate the effects of estrogen and estrogen receptor α (ERα) and β (ERβ) on the expression of visfatin and retinol-binding protein 4 (RBP4) by treating 3T3-L1 adipocytes with estradiol (E2), estrogen receptor agonists and antagonists. Mature adipocytes were exposed to E2, the ERα agonist, 4,4',4''-(4-propyl-[1H]-pyrazole-1,3,5-triyl)trisphenol (PPT), the ERβ agonist, 2,3-bis(4-hydroxyphenyl)-propionitrile (DPN), E2 with the ERα antagonist, 1,3-bis(4-hydroxyphenyl)-4-methyl-5-[4-(2-piperidinylethoxy)phenol]-1H-pyrazole dihydrochloride (MPP), and E2 with the ERβ antagonist, (5R, 11R)-5,11-diethyl-5,6,11,12-tetrahydro-2,8-chrysenediol [(R,R)-THC], at various concentrations. To determine the effects of ER subtypes on the expression of adipokines, quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR) and western blot analyses were performed. E2 concentrations of 10-5 and 10-6 mol/l induced a statistically significant increase in the expression of RBP4 (P=0.012 and P=0.011, respectively). In the cells treated with 10-5 mol/l PPT, RBP4 expression significantly increased (P<0.05) in a dose-dependent manner. Treatment with the ERα antagonist, MPP (10-5 mol/l), and E2 suppressed the expression of RBP4 (P=0.032). However, the expression of RBP4 was not significantly altered when the cells were treated with the ERβ agonist or antagonist. The expression of visfatin was not affected by different concentrations of E2 and ERs. 17β-estradiol significantly increased the secretion of RBP4 and upregulated RBP4 expression via ERα but not ERβ in 3T3-L1 adipocytes. RBP4 expression was regulated by estrogen in the 3T3-L1 adipocytes and this effect was selectively mediated by ERα.
authors.affiliation: Department of Obstetrics and Gynecology, Konyang University Hospital, College of Medicine, Konyang University, Daejeon 302-718, Republic of Korea.
authors.forename: Un Suk
authors.initials: US
authors.lastname: Jung
authors.forename: Kang Jin
authors.initials: KJ
authors.lastname: Jeong
authors.forename: Jae Ku
authors.initials: JK
authors.lastname: Kang
authors.forename: Kyongwook
authors.initials: K
authors.lastname: Yi
authors.forename: Jung-Ho
authors.initials: JH
authors.lastname: Shin
authors.forename: Hong Seog
authors.initials: HS
authors.lastname: Seo
authors.forename: Tak
authors.initials: T
authors.lastname: Kim
authors.forename: Sun-Haeng
authors.initials: SH
authors.lastname: Kim
authors.forename: Jun-Young
authors.initials: JY
authors.lastname: Hur
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D004958:Estradiol / Q000494:pharmacology; D004965:Estrogen Antagonists / Q000494:pharmacology; D047628:Estrogen Receptor alpha / Q000378:metabolism*; D047629:Estrogen Receptor beta / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000187:drug effects; D051379:Mice; D054409:Nicotinamide Phosphoribosyltransferase / Q000235:genetics*; D054839:Retinol-Binding Proteins, Plasma / Q000235:genetics*
chemical_list: D004965:Estrogen Antagonists; D047628:Estrogen Receptor alpha; D047629:Estrogen Receptor beta; C502487:Rbp4 protein, mouse; D054839:Retinol-Binding Proteins, Plasma; D004958:Estradiol; D054409:Nicotinamide Phosphoribosyltransferase