title: Comparison of gene expression profiles in granulosa and cumulus cells after ovulation induction with either human chorionic gonadotropin or a gonadotropin-releasing hormone agonist trigger.
abstract: OBJECTIVE
  To explore differences in follicle transcriptomes in patients having oocyte maturation with either a bolus of hCG or GnRHa.
  
  
  METHODS
  Cumulus cells (CC) and mural granulosa cells (MGC) were isolated from preovulatory follicles in patients undergoing controlled ovarian stimulation, prospectively randomized to GnRHa or hCG triggering.
  
  
  METHODS
  University-based facilities for clinical services and research.
  
  
  METHODS
  Twenty women with indication for IVF or intracytoplasmic sperm injection treatment were randomly allocated to hCG or GnRH agonist (GnRHa) trigger.
  
  
  METHODS
  MGC and CC were collected from individual follicles in connection with oocyte retrieval.
  
  
  METHODS
  RNA was extracted, labeled, amplified, and hybridized on HumanGene1.0ST GeneChip Affymetrix array. Expression data were robust multichip average normalized and compared using Partek and Ingenuity software. Array data were confirmed with reverse transcription-polymerase chain reaction analysis.
  
  
  RESULTS
  Comparing the transcriptomes between the groups, 391 and 252 genes were differentially expressed (fold change >1.5) in CC and MGC, respectively. The enriched bionetworks showed that CC genes highly represented "lipid metabolism and small molecule biochemistry" (network score, 41), while in MGC, the top network was "cardiovascular development and function and cellular movement" (network score, 50). For both CC and MGC, the regulator analysis suggested LH as the upstream regulator for the difference observed. In CC, the LH receptor was more highly expressed after GnRHa trigger, while in MGC, genes involved in angiogenesis such as angiopoietin 1 and semaphorin 3A were down- and up-regulated, respectively, in GnRHa- as compared with hCG-triggered patients.
  
  
  CONCLUSIONS
  The comparisons between somatic cell transcriptomes from GnRHa- and hCG-triggered follicles showed significant functional differences in both CC (steroidogenesis) and MGC (angiogenesis) compartments.
authors.affiliation: Laboratory of Reproductive Biology, Rigshospitalet, Copenhagen, Denmark.
authors.forename: Tanni
authors.initials: T
authors.lastname: Borgbo
authors.forename: Betina Boel
authors.initials: BB
authors.lastname: Povlsen
authors.forename: Claus Yding
authors.initials: CY
authors.lastname: Andersen
authors.forename: Rehannah
authors.initials: R
authors.lastname: Borup
authors.forename: Peter
authors.initials: P
authors.lastname: Humaidan
authors.forename: Marie Louise
authors.initials: ML
authors.lastname: Grøndahl
mesh_terms: D002064:Buserelin / Q000008:administration & dosage*; D006063:Chorionic Gonadotropin / Q000008:administration & dosage*; D054885:Cumulus Cells / Q000187:drug effects* / Q000378:metabolism; D003718:Denmark; D004334:Drug Administration Schedule; D005260:Female; D005300:Fertility Agents, Female / Q000008:administration & dosage*; D020869:Gene Expression Profiling / Q000379:methods; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D053263:Gene Regulatory Networks / Q000187:drug effects; D007987:Gonadotropin-Releasing Hormone / Q000819:agonists* / Q000378:metabolism; D006107:Granulosa Cells / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis; D054315:Oocyte Retrieval; D010062:Ovulation Induction / Q000379:methods*; D011446:Prospective Studies; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D006063:Chorionic Gonadotropin; D005300:Fertility Agents, Female; D007987:Gonadotropin-Releasing Hormone; D002064:Buserelin
keywords: Cumulus cells; GnRHa trigger; gene expression profile; granulosa cells; hCG trigger