title: Backbone-modified molecular beacons for highly sensitive and selective detection of microRNAs based on duplex specific nuclease signal amplification.
abstract: Based on backbone-modified molecular beacons and duplex-specific nuclease, we have developed a target recycling amplification method for highly sensitive and selective miRNA detection. The combination of a low fluorescence background of 2-OMe-RNA modified MB and nuclease-assisted signal amplification leads to ultrahigh assay sensitivity, and the powerful discriminating ability of MB enables the differentiation of highly similar miRNAs with one-base difference, both of which are of great significance to miRNA detection.
authors.affiliation: The Key Laboratory of Analytical Science, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Lin
authors.forename: Cheng
authors.initials: C
authors.lastname: Zhang
authors.forename: Yishun
authors.initials: Y
authors.lastname: Huang
authors.forename: Zhi
authors.initials: Z
authors.lastname: Zhu
authors.forename: Xi
authors.initials: X
authors.lastname: Chen
authors.forename: Chaoyong James
authors.initials: CJ
authors.lastname: Yang
mesh_terms: D004720:Endonucleases / Q000378:metabolism*; D035683:MicroRNAs / Q000032:analysis*; D021141:Nucleic Acid Amplification Techniques; D012313:RNA / Q000737:chemistry / Q000378:metabolism; D013050:Spectrometry, Fluorescence
chemical_list: D035683:MicroRNAs; D012313:RNA; D004720:Endonucleases