title: Identification of the recognition sequence and target proteins for DJ-1 protease.
abstract: DJ-1, the product of familial Parkinson's disease gene and an oncogene, is a cysteine protease which plays a role in anti-oxidative stress reaction. In this study, we identified the recognition sequence for DJ-1 protease by using recombinant DJ-1 and a peptide library. Protease activity of DJ-1 lacking C-terminal α-helix (DJ-1ΔH9) was stronger than that of full-sized DJ-1, and the most susceptible sequence digested by DJ-1ΔH9 was valine-lysine-valine-alanine (VKVA) under the optimal conditions of pH 5.5 and 0 mM NaCl. Divalent ions, especially Cu²⁺, were inhibitory to DJ-1's protease activity. c-abl oncogene 1 product (ABL1) and kinesin family member 1B (KIF1B) containing VKVA were digested by DJ-1ΔH9.
authors.affiliation: Graduate School of Pharmaceutical Sciences, Hokkaido University, Kita-ku, Sapporo 060-0812, Japan.
authors.forename: Hitomi
authors.initials: H
authors.lastname: Mitsugi
authors.forename: Takeshi
authors.initials: T
authors.lastname: Niki
authors.forename: Kazuko
authors.initials: K
authors.lastname: Takahashi-Niki
authors.forename: Kyoko
authors.initials: K
authors.lastname: Tanimura
authors.forename: Kumiko
authors.initials: K
authors.lastname: Yoshizawa-Kumagaye
authors.forename: Masahiko
authors.initials: M
authors.lastname: Tsunemi
authors.forename: Sanae M M
authors.initials: SM
authors.lastname: Iguchi-Ariga
authors.forename: Hiroyoshi
authors.initials: H
authors.lastname: Ariga
mesh_terms: D000975:Antioxidants / Q000737:chemistry; D003300:Copper / Q000737:chemistry; D057056:Cysteine Proteases / Q000737:chemistry; D004926:Escherichia coli / Q000378:metabolism; D006801:Humans; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D047908:Intracellular Signaling Peptides and Proteins / Q000378:metabolism*; D016547:Kinesins / Q000737:chemistry; D015513:Oncogene Proteins / Q000378:metabolism*; D018384:Oxidative Stress; D019151:Peptide Library; D011228:Prealbumin / Q000737:chemistry; D011485:Protein Binding; D000071617:Protein Deglycase DJ-1; D017434:Protein Structure, Tertiary; D016315:Proto-Oncogene Proteins c-abl / Q000737:chemistry; D011994:Recombinant Proteins / Q000737:chemistry; D013696:Temperature
chemical_list: D000975:Antioxidants; D047908:Intracellular Signaling Peptides and Proteins; C091053:KIF1B protein, human; D015513:Oncogene Proteins; D019151:Peptide Library; D011228:Prealbumin; D011994:Recombinant Proteins; D003300:Copper; D016315:Proto-Oncogene Proteins c-abl; C105131:PARK7 protein, human; D000071617:Protein Deglycase DJ-1; D057056:Cysteine Proteases; D016547:Kinesins
keywords: Biochemistry; DJ-1; Protease