title: In vitro degradation and cytotoxicity response of Mg-4% Zn-0.5% Zr (ZK40) alloy as a potential biodegradable material.
abstract: Mg-4 wt.% Zn-0.5 wt.% Zr (ZK40) alloy was studied as a candidate material for biodegradable metallic implants in terms of its biocorrosion resistance, mechanical properties and cytocompatibility. The corrosion characteristics of ZK40 alloy were assessed by potentiodynamic polarization and immersion testing in DMEM+10% FBS solution. Analysis of the degradation characteristics by potentiodynamic polarization measurements shows the corrosion rates of ZK40 alloy in as-cast and solution treatment (T4) condition were slightly higher than those of pure Mg or as-drawn AZ31. Determination of the corrosion rate by the weight loss technique reveals that the as-cast ZK40 resulted in slower degradation than other alloy specimens after 7 days of immersion but exhibited accelerated degradation after 14 and 21 days, respectively. T4-treated ZK40 exhibited stable degradation rates compared to as-cast ZK40 and close to those of pure Mg and AZ31 during immersion testing for 14 and 21 days. In order to examine the in vitro cytocompatibility of ZK40 alloy, live/dead cell viability assay and indirect MTT assay were performed using a murine osteoblast-like cell line (MC3T3). After 3 days of direct culture of MC3T3 on ZK40 alloys the live/dead assay indicated favorable cell viability and attachment. The degradation product of ZK40 also showed minimal cytotoxicity when assessed in indirect MTT assay. The mechanical properties of the as-cast and T4-treated ZK40 alloy were superior to those of pure Mg and comparable to as-drawn AZ31. Solution treatment did not significantly enhance the cytocompatibility and mechanical properties of ZK40 alloy. Overall, the ZK40 alloy exhibited favorable cytocompatibility, biocorrosion, and mechanical properties rendering it a potential candidate for degradable implant applications.
authors.affiliation: Department of Bioengineering, University of Pittsburgh, 848 Benedum Hall, 3700 O'Hara Street, Pittsburgh, PA 15213, USA.
authors.forename: Daeho
authors.initials: D
authors.lastname: Hong
authors.forename: Partha
authors.initials: P
authors.lastname: Saha
authors.forename: Da-Tren
authors.initials: DT
authors.lastname: Chou
authors.forename: Boeun
authors.initials: B
authors.lastname: Lee
authors.forename: Boyce E
authors.initials: BE
authors.lastname: Collins
authors.forename: Zongqing
authors.initials: Z
authors.lastname: Tan
authors.forename: Zhongyun
authors.initials: Z
authors.lastname: Dong
authors.forename: Prashant N
authors.initials: PN
authors.lastname: Kumta
mesh_terms: D020341:Absorbable Implants; D000497:Alloys / Q000633:toxicity*; D000818:Animals; D001672:Biocompatible Materials / Q000633:toxicity*; D016923:Cell Death / Q000187:drug effects; D002460:Cell Line; D003343:Corrosion; D003470:Culture Media / Q000494:pharmacology; D055664:Electrochemical Techniques; D055595:Mechanical Phenomena / Q000187:drug effects; D051379:Mice; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects / Q000648:ultrastructure; D013052:Spectrometry, X-Ray Emission; D040521:Subcutaneous Tissue / Q000187:drug effects / Q000473:pathology / Q000648:ultrastructure; D014057:Tomography, X-Ray Computed; D014961:X-Ray Diffraction
chemical_list: D000497:Alloys; D001672:Biocompatible Materials; D003470:Culture Media; C588232:ZK40 alloy
keywords: Biodegradation; Cytocompatibility; Magnesium; Mechanical properties; Mg-Zn-Zr