title: Confirmation of quinolone-induced formation of gyrase-DNA conjugates using AFM.
abstract: It was demonstrated in our studies that norfloxacin, a representative member of quinolone antibiotics, can indeed stabilize the gyrase-DNA complex formed during enzymatic cycle. In addition, the formation of the drug-induced complex has been firstly visualized through our atomic force microscopic examination.
authors.affiliation: Division of Chemistry and Biological Chemistry, Nanyang Technological University, 21 Nanyang Link, Singapore 637371, Singapore. hzhang11@e.ntu.edu.sg
authors.forename: Hao
authors.initials: H
authors.lastname: Zhang
authors.forename: Juanjuan
authors.initials: J
authors.lastname: Guo
authors.forename: Dawei
authors.initials: D
authors.lastname: Li
authors.forename: Magdeline Tao Tao
authors.initials: MT
authors.lastname: Ng
authors.forename: Jasmine Yiqin
authors.initials: JY
authors.lastname: Lee
authors.forename: Bei
authors.initials: B
authors.lastname: Lv
authors.forename: Chee Wee
authors.initials: CW
authors.lastname: Ng
authors.forename: Selvi
authors.initials: S
authors.lastname: Lee
authors.forename: Fangwei
authors.initials: F
authors.lastname: Shao
authors.forename: Tianhu
authors.initials: T
authors.lastname: Li
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D027081:DNA Gyrase / Q000378:metabolism*; D004587:Electrophoresis, Agar Gel; D004795:Enzyme Stability / Q000187:drug effects; D018625:Microscopy, Atomic Force; D015394:Molecular Structure; D009643:Norfloxacin / Q000494:pharmacology; D011804:Quinolines / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D011804:Quinolines; D027081:DNA Gyrase; D009643:Norfloxacin
keywords: AFM; Complexes; Gyrase; Norfloxacin; Quinolone