title: Oncolytic adenoviruses: design, generation, and experimental procedures.
abstract: Oncolytic adenoviruses are designed to take advantage of the virus' native ability to replicate in cancer cells to induce oncolysis. Subsequently, the released viral progeny spread and kill the neighboring cancer cells. These characteristics, together with the ability of adenovirus to infect a broad spectrum of cells, its well understood replication machinery, and relative ease of manufacture have led to the intensive use of adenovirus as an anticancer agent. This unit describes cloning strategies, procedures to turn the intended design into virus, and quality analyses of resultant adenoviral vectors. Most of these procedures were optimized especially for oncolytic adenoviral vectors.
authors.affiliation: Division of Basic and Translational Research, Department of Surgery, University of Minnesota, Minneapolis, Minnesota, USA.
authors.forename: Julia
authors.initials: J
authors.lastname: Davydova
authors.forename: Masato
authors.initials: M
authors.lastname: Yamamoto
mesh_terms: D000260:Adenoviruses, Human / Q000235:genetics* / Q000302:isolation & purification; D002461:Cell Line, Transformed; D045744:Cell Line, Tumor; D005822:Genetic Vectors / Q000235:genetics; D016679:Genome, Viral; D057809:HEK293 Cells; D006801:Humans; D009154:Mutation; D009369:Neoplasms / Q000473:pathology / Q000628:therapy / Q000821:virology; D050130:Oncolytic Virotherapy / Q000379:methods*; D050504:Oncolytic Viruses / Q000235:genetics* / Q000302:isolation & purification; D011401:Promoter Regions, Genetic / Q000235:genetics; D014162:Transfection; D014771:Virion / Q000235:genetics; D014779:Virus Replication