title: Solid lipid nanoparticles of anticancer drug andrographolide: formulation, in vitro and in vivo studies.
abstract: Diterpenoidal anti-cancer drug andrographolide (AD) was encapsulated into solid lipid nanoparticle (SLN) because of poor aqueous solubility and high lipophilicity. AD-SLNs were prepared by solvent injection method and characterized for droplet size, surface morphology, zeta potential, etc. In vitro drug release was carried out by dialysis-membrane method. A pharmacokinetic study was performed by UPLC/Q-TOF-MS method to determine the maximum plasma concentration (Cmax), area under the curve (AUC), etc. There was an improvement in Cmax and AUC of AD-SLNs when compared with AD, thereby enhancing the bioavailability of AD. The tmax was increased than that of AD suspension, indicating the sustained release pattern of AD-SLNs. The antitumor activity was carried out on Balb/c mice showing better results with AD-SLNs as compared to AD. Thus, the AD-loaded SLNs would be useful for delivering poorly water-soluble AD with enhanced bioavailability and improved antitumor activity.
authors.affiliation: Department of Pharmaceutics, Faculty of Pharmacy, Jamia Hamdard , New Delhi , India .
authors.forename: Rabea
authors.initials: R
authors.lastname: Parveen
authors.forename: F J
authors.initials: FJ
authors.lastname: Ahmad
authors.forename: Z
authors.initials: Z
authors.lastname: Iqbal
authors.forename: M
authors.initials: M
authors.lastname: Samim
authors.forename: Sayeed
authors.initials: S
authors.lastname: Ahmad
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry* / Q000493:pharmacokinetics / Q000494:pharmacology*; D019540:Area Under Curve; D001682:Biological Availability; D045744:Cell Line, Tumor; D002626:Chemistry, Pharmaceutical / Q000379:methods; D004224:Diterpenes / Q000737:chemistry* / Q000493:pharmacokinetics / Q000494:pharmacology*; D005260:Female; D006801:Humans; D008055:Lipids / Q000737:chemistry* / Q000493:pharmacokinetics / Q000494:pharmacology*; D061986:MCF-7 Cells; D051379:Mice; D008807:Mice, Inbred BALB C; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D012995:Solubility; D012997:Solvents / Q000737:chemistry; D013535:Suspensions / Q000737:chemistry / Q000494:pharmacology; D014867:Water / Q000737:chemistry
chemical_list: D000970:Antineoplastic Agents; D004224:Diterpenes; D008055:Lipids; D012997:Solvents; D013535:Suspensions; D014867:Water; C030419:andrographolide
keywords: Andrographolide; UPLC/Q-TOF-MS method; antitumor activity; pharmacokinetics; solid lipid nanoparticles