title: Production of bioethanol from fermented sugars of sugarcane bagasse produced by lignocellulolytic enzymes of Exiguobacterium sp. VSG-1.
abstract: Exiguobacterium sp. VSG-1 was isolated from the soil sample and characterized for the production of lignocellulolytic enzymes. Production of these enzymes by the strain VSG-1 was carried out using steam-exploded sugarcane bagasse (SCB) and found to secrete cellulase, pectinase, mannanase, xylanase, and tannase. The growth and enzyme production were found to be optimum at pH 9.0 and 37 °C. Upon steam explosion of SCB, the cellulose increased by 42 %, whereas hemicelluloses and lignin decreased by 40 and 62 %, respectively. Enzymatic hydrolysis of steam-exploded SCB yielded 640 g/l of total sugars. Fermentation of sugars produced from pretreated SCB was carried out by using Saccharomyces cerevisiae at pH 5.0 and 30 °C. The alcohol produced was calculated and found to be 62.24 g/l corresponding to 78 % of the theoretical yield of ethanol. Hence, the strain VSG-1 has an industrial importance for the production of fermentable sugars for biofuels.
authors.affiliation: Department of Biochemistry, Gulbarga University, Gulbarga, 585106, Karnataka, India.
authors.forename: S
authors.initials: S
authors.lastname: Vijayalaxmi
authors.forename: K A
authors.initials: KA
authors.lastname: Anu Appaiah
authors.forename: S K
authors.initials: SK
authors.lastname: Jayalakshmi
authors.forename: V H
authors.initials: VH
authors.lastname: Mulimani
authors.forename: K
authors.initials: K
authors.lastname: Sreeramulu
mesh_terms: D056449:Bacillales / Q000187:drug effects / Q000201:enzymology* / Q000254:growth & development / Q000378:metabolism; D056804:Biofuels / Q000382:microbiology*; D002482:Cellulose / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation; D005947:Glucose / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008031:Lignin / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D031786:Saccharum / Q000737:chemistry*; D012965:Sodium Chloride / Q000494:pharmacology; D013696:Temperature
chemical_list: D056804:Biofuels; C036909:lignocellulose; D000431:Ethanol; D012965:Sodium Chloride; D002482:Cellulose; D008031:Lignin; C027433:bagasse; D005947:Glucose