title: Metabolite profiling of the short-term responses of rice leaves (Oryza sativa cv. Ilmi) cultivated under different LED lights and its correlations with antioxidant activities.
abstract: Metabolite profiling of rice leaves (Oryza sativa cv. Ilmi) was performed to investigate the short-term responses to different light-emitting diode (LED) lights, blue (B), green (G), red (R), white (W), shade (S), by using gas chromatography-ion trap-mass spectrometry (GC-IT-MS) and ultra-performance liquid chromatography-quadrupole-time-of-flight-mass spectrometry (UPLC-Q-TOF-MS) with multivariate analysis. Clear grouping patterns of each light-grown sample, except G and W, were shown in partial least squares-discriminant analysis (PLS-DA). Thirty-two primary metabolites and eleven secondary metabolites were selected and visualized using heatmap. Antioxidant activities of rice leaves followed the order B=W=G>R>S and isoorientin-2''-O-glucoside, isovitexin-2''-O-glucoside, isoorientin-2''-O-(6'''-ρ-coumaroyl)-glucoside, and isoscoparin-2''-O-glucoside showed similar relative differences and had higher Pearson's correlation coefficients than other metabolites in correlation network. According to the orthogonal projection to latent structures-discriminant analysis (OPLS-DA) between B and R, the levels of amino acids, organic acids, fatty acids, and flavonoid glycosides were relatively high in B, whereas the glucose and fructose levels were high in R.
authors.affiliation: Department of Bioscience and Biotechnology, Konkuk University, Seoul 143-701, Republic of Korea.
authors.forename: Eun Sung
authors.initials: ES
authors.lastname: Jung
authors.forename: Sarah
authors.initials: S
authors.lastname: Lee
authors.forename: Sun-Hyung
authors.initials: SH
authors.lastname: Lim
authors.forename: Sun-Hwa
authors.initials: SH
authors.lastname: Ha
authors.forename: Kwang-Hyeon
authors.initials: KH
authors.lastname: Liu
authors.forename: Choong Hwan
authors.initials: CH
authors.lastname: Lee
mesh_terms: D000975:Antioxidants / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D008401:Gas Chromatography-Mass Spectrometry; D008027:Light; D053858:Metabolic Networks and Pathways; D055432:Metabolomics; D012275:Oryza / Q000378:metabolism* / Q000528:radiation effects; D018515:Plant Leaves / Q000378:metabolism / Q000528:radiation effects
chemical_list: D000975:Antioxidants
keywords: Antioxidant activity; GC–IT-MS; LED lights; Metabolite profiling; Oryza sativa; UPLC–Q-TOF-MS