title: Using an isolated rat kidney model to identify kidney origin proteins in urine.
abstract: The use of targeted proteomics to identify urinary biomarkers of kidney disease in urine can avoid the interference of serum proteins. It may provide better sample throughput, higher sensitivity, and specificity. Knowing which urinary proteins to target is essential. By analyzing the urine from perfused isolated rat kidneys, 990 kidney origin proteins with human analogs were identified in urine. Of these proteins, 128 were not found in normal human urine and may become biomarkers with zero background. A total of 297 proteins were not found in normal human plasma. These proteins will not be influenced by other normal organs and will be kidney specific. The levels of 33 proteins increased during perfusion with an oxygen-deficient solution compared to those perfused with oxygen. The 75 proteins in the perfusion-driven urine have a significantly increased abundance ranking compared to their ranking in normal human urine. When compared with existing candidate biomarkers, over ninety percent of the kidney origin proteins in urine identified in this study have not been examined as candidate biomarkers of kidney diseases.
authors.affiliation: Department of Physiology and Pathophysiology, National Key Laboratory of Medical Molecular Biology. Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences/Peking Union Medical College, Beijing, China.
authors.forename: Lulu
authors.initials: L
authors.lastname: Jia
authors.forename: Xundou
authors.initials: X
authors.lastname: Li
authors.forename: Chen
authors.initials: C
authors.lastname: Shao
authors.forename: Lilong
authors.initials: L
authors.lastname: Wei
authors.forename: Menglin
authors.initials: M
authors.lastname: Li
authors.forename: Zhengguang
authors.initials: Z
authors.lastname: Guo
authors.forename: Zhihong
authors.initials: Z
authors.lastname: Liu
authors.forename: Youhe
authors.initials: Y
authors.lastname: Gao
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000652:urine; D004195:Disease Models, Animal; D005786:Gene Expression Regulation; D006801:Humans; D007668:Kidney / Q000378:metabolism*; D008297:Male; D010477:Perfusion; D011507:Proteinuria / Q000378:metabolism* / Q000652:urine; D040901:Proteomics; D051381:Rats; D017207:Rats, Sprague-Dawley; D017386:Sequence Homology, Amino Acid; D014551:Urinary Tract / Q000378:metabolism
chemical_list: D015415:Biomarkers