title: Evaluation of anti-resistant activity of Auklandia (Saussurea lappa) root against some human pathogens.
abstract: OBJECTIVE
  The antimicrobial activity of the ethanol extract of the Auklandia (Saussurea lappa)root plant was investigated to verify its medicinal use in the treatment of microbial infections.
  
  
  METHODS
  The antimicrobial activity of the ethanol extract was tested against clinical isolates of some multidrug-resistant bacteria using the agar well diffusion method. Commercial antibiotics were used as positive reference standards to determine the sensitivity of the clinical isolates.
  
  
  RESULTS
  The extracts showed significant inhibitory activity against clinical isolates of methicillin resistant Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Klebsiella pneumonia, Extended Spectrum Beta-Lactemase, Acinetobacter baumannii. The minimum inhibitory concentration values obtained using the agar dilution test ranged from 2.0 µg/µL-12.0 µg/µL. In the contrary the water extract showed no activity at all against the tested isolates. Furthermore, the results obtained by examining anti-resistant activity of the plant ethanolic extract showed that at higher concentration of the plant extract (12 µg) all tested bacteria isolates were inhibited with variable inhibition zones similar to those obtained when we applied lower extract concentration using the well diffusion assay.
  
  
  CONCLUSIONS
  The results demonstrated that the crude ethanolic extract of the Auklandia (Saussurea lappa) root plant has a wide spectrum of activity suggesting that it may be useful in the treatment of infections caused by the above clinical isolates (human pathogens).
authors.affiliation: Department of Microbiology and Immunology, College of Medicine and Health Sciences, Sultan Qaboos University, Oman.
authors.forename: Sidgi Syed Anwer
authors.initials: SS
authors.lastname: Hasson
authors.forename: Mohammed Saeed
authors.initials: MS
authors.lastname: Al-Balushi
authors.forename: Khazina
authors.initials: K
authors.lastname: Alharthy
authors.forename: Juma Zaid
authors.initials: JZ
authors.lastname: Al-Busaidi
authors.forename: Muna Suliman
authors.initials: MS
authors.lastname: Aldaihani
authors.forename: Mohammed Shafeeq
authors.initials: MS
authors.lastname: Othman
authors.forename: Elias Antony
authors.initials: EA
authors.lastname: Said
authors.forename: Omar
authors.initials: O
authors.lastname: Habal
authors.forename: Talal Abdullah
authors.initials: TA
authors.lastname: Sallam
authors.forename: Ali Abdullah
authors.initials: AA
authors.lastname: Aljabri
authors.forename: Mohamed
authors.initials: M
authors.lastname: Ahmedidris
mesh_terms: D040981:Acinetobacter baumannii / Q000187:drug effects; D001419:Bacteria / Q000187:drug effects*; D001424:Bacterial Infections / Q000188:drug therapy; D004926:Escherichia coli / Q000187:drug effects; D006801:Humans; D007711:Klebsiella pneumoniae / Q000187:drug effects; D055624:Methicillin-Resistant Staphylococcus aureus / Q000187:drug effects; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D018517:Plant Roots; D011550:Pseudomonas aeruginosa / Q000187:drug effects; D030441:Saussurea
chemical_list: D010936:Plant Extracts
keywords: Anti-resistant activity; Antimicrobial activity; Auklandia; Ethanol extract; Minimum inhibitory concentration (MIC); Saussurea lappa