title: [The influence of rhAng-1 on endothelial junctions of BBB after focal cerebral ischemia/reperfusion in rats].
abstract: OBJECTIVE
  This study was performed to determine whether recombinant human angiopoietin-1 (rhAng-1) decreases the permeability of the blood-brain barrier (BBB) in focal cerebral ischemia and reperfusion rats, whether RhAng-1 opens the BBB by affecting tight junction associated proteins zonnula occludin-1 (ZO-1), occludin and adherens junction protein vascular endothelial (VE)-cadherin.
  
  
  METHODS
  The rats were divided into eight groups randomly( n = 10): (1) sham-operated group; (2) ischemia group; (3)-(5) ischemia/reperfusion (middle cerebral artery occlusion and reperfusion (MCAO/R) 12 h, 48 h, and 7 days) and 0.9% saline groups; (6)-(8) ischemia/reperfusion (MCAO/R 12 h, 48 h, and 7 days) and rhAng-1 groups. The Bee permeability was assessed by Evans blue extravasation. The messenger RNA and protein expressions of ZO-1, occludin, and VE-cadherin were determined by reverse transcription-polymerase chain reaction, Western blot, and immunohistochemistry assays.
  
  
  RESULTS
  The BBB permeability and the brain infarct volume were significantly decreased after rhAng-1 injection. The expressions of ZO-1, occludin, and VE-cadherin were increased after rhAng-1 injection.
  
  
  CONCLUSIONS
  rhAng-1 may decrease the permeability of BBB in MCAO/R rats by upregulation of ZO-1, occludin, and VE-cadherin.
authors.affiliation: Harbin Medical University-Daqing, Daqing 163319, China.
authors.forename: Hang
authors.initials: H
authors.lastname: Yu
authors.forename: Qing-Bo
authors.initials: QB
authors.lastname: Jia
authors.forename: Yi-Xue
authors.initials: YX
authors.lastname: Xue
mesh_terms: D042683:Angiopoietin-1 / Q000494:pharmacology*; D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D001812:Blood-Brain Barrier / Q000166:cytology / Q000378:metabolism*; D002545:Brain Ischemia / Q000378:metabolism*; D015820:Cadherins / Q000378:metabolism; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects; D006801:Humans; D008297:Male; D062793:Occludin / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D011994:Recombinant Proteins / Q000494:pharmacology; D015427:Reperfusion Injury / Q000378:metabolism*; D062826:Zonula Occludens-1 Protein / Q000378:metabolism; D000099152:Cadherin 5
chemical_list: D042683:Angiopoietin-1; D015703:Antigens, CD; D015820:Cadherins; D062793:Occludin; D011994:Recombinant Proteins; D062826:Zonula Occludens-1 Protein; D000099152:Cadherin 5; C568987:Ocln protein, rat; C569044:Tjp1 protein, rat