title: Fullerol-fluorescein isothiocyanate-concanavalin agglutinin phosphorescent sensor for the detection of alpha-fetoprotein and forecast of human diseases.
abstract: Based on the reaction of the active -OH group in fullerol (F) with the dissociated -COOH group in fluorescein isothiocyanate (FITC) to form an F-FITC and the enhanced effect of N, N-dimethylaniline (DMA) on phosphorescence signal of F-FITC, a new phosphorescent labeling reagent (DMA-F-FITC) was developed. What's more, a phosphorescent sensor for the determination of alpha-fetoprotein variant (AFP-V) has been designed via the coupling technique of the high sensitivity for affinity adsorption-solid substrate-room temperature phosphorimetry (AA-SS-RTP) with the strong specificity reaction between DMA-F-FITC-Con A and AFP-V. The DMA-F-FITC increased the number of luminescent molecules in the biological target which improved the sensitivity of phosphorescent sensor. The proposed sensor was responsive, simple, selective and sensitive, and it has been applied to the determination of trace AFP-V in human serum and the forecast of human diseases using phosphorescence emission wavelength of F or FITC, with the results agreed well with those obtained by enzyme-linked immunoassay (ELISA). Meanwhile, the mechanisms for the labeling reaction and the sensing detection of AFP-V were discussed.
authors.affiliation: Department of Chemistry and Environmental Science, Zhangzhou Normal College, Zhangzhou 363000, PR China. zzsyliujiaming@163.com
authors.forename: Jia-ming
authors.initials: JM
authors.lastname: Liu
authors.forename: Li-ping
authors.initials: LP
authors.lastname: Lin
authors.forename: Shu-lian
authors.initials: SL
authors.lastname: Jiang
authors.forename: Ma Lin
authors.initials: ML
authors.lastname: Cui
authors.forename: Li
authors.initials: L
authors.lastname: Jiao
authors.forename: Xiao Yang
authors.initials: XY
authors.lastname: Zhang
authors.forename: Li-hong
authors.initials: LH
authors.lastname: Zhang
authors.forename: Zhi Yong
authors.initials: ZY
authors.lastname: Zheng
authors.forename: Xuan
authors.initials: X
authors.lastname: Lin
authors.forename: Shao-qin
authors.initials: SQ
authors.lastname: Lin
mesh_terms: D000814:Aniline Compounds; D015374:Biosensing Techniques; D003208:Concanavalin A / Q000737:chemistry*; D004194:Disease; D016650:Fluorescein-5-isothiocyanate / Q000031:analogs & derivatives* / Q000737:chemistry; D037741:Fullerenes / Q000737:chemistry*; D006801:Humans; D006813:Humidity; D007202:Indicators and Reagents; D057230:Limit of Detection; D049449:Luminescence; D010100:Oxygen / Q000737:chemistry; D013696:Temperature; D013997:Time Factors; D000509:alpha-Fetoproteins / Q000032:analysis*
chemical_list: D000814:Aniline Compounds; D037741:Fullerenes; D007202:Indicators and Reagents; D000509:alpha-Fetoproteins; C042784:fluorescein isothiocyanate-concanavalin A; C408042:fullerol C60(OH)24; D003208:Concanavalin A; C015157:N,N-dimethylaniline; D016650:Fluorescein-5-isothiocyanate; D010100:Oxygen
keywords: Alpha-fetoprotein variant; Forecast and diagnosis of human diseases; Fullerol-fluorescein isothiocyanate; Phosphorescent labeling reagent; Phosphorescent sensor