title: An alternative beads-on-a-string chromatin architecture in Thermococcus kodakarensis.
abstract: We have applied chromatin sequencing technology to the euryarchaeon Thermococcus kodakarensis, which is known to possess histone-like proteins. We detect positioned chromatin particles of variable sizes associated with lengths of DNA differing as multiples of 30 bp (ranging from 30 bp to >450 bp) consistent with formation from dynamic polymers of the archaeal histone dimer. T. kodakarensis chromatin particles have distinctive underlying DNA sequence suggesting a genomic particle-positioning code and are excluded from gene-regulatory DNA suggesting a functional organization. Beads-on-a-string chromatin is therefore conserved between eukaryotes and archaea but can derive from deployment of histone-fold proteins in a variety of multimeric forms.
authors.affiliation: Department of Bacteriology, Osaka Dental University, Osaka 573-1121, Japan.
authors.forename: Hugo
authors.initials: H
authors.lastname: Maruyama
authors.forename: Janet C
authors.initials: JC
authors.lastname: Harwood
authors.forename: Karen M
authors.initials: KM
authors.lastname: Moore
authors.forename: Konrad
authors.initials: K
authors.lastname: Paszkiewicz
authors.forename: Samuel C
authors.initials: SC
authors.lastname: Durley
authors.forename: Hisanori
authors.initials: H
authors.lastname: Fukushima
authors.forename: Haruyuki
authors.initials: H
authors.lastname: Atomi
authors.forename: Kunio
authors.initials: K
authors.lastname: Takeyasu
authors.forename: Nicholas A
authors.initials: NA
authors.lastname: Kent
mesh_terms: D019843:Archaeal Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D019641:DNA, Archaeal / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D020745:Genome, Archaeal; D006657:Histones / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D009690:Nucleic Acid Conformation; D009707:Nucleosomes / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D017510:Protein Folding; D055503:Protein Multimerization; D019712:Thermococcus / Q000235:genetics* / Q000378:metabolism
chemical_list: D019843:Archaeal Proteins; D019641:DNA, Archaeal; D006657:Histones; D009707:Nucleosomes