title: In vitro binding and bimolecular fluorescence complementation assays suggest an interaction between tomato mosaic virus coat protein and tobacco chloroplast ferredoxin I.
abstract: Symptom development of a viral plant disease results from molecular interactions between the virus and its host plant. Tomato mosaic virus coat protein (ToMV CP) not only plays a major role in virion assembly and long-distance movement but is also responsible for symptom development in ToMV-infected plants. This study provides evidence that chloroplast ferredoxin I (Fd I) interacts with ToMV CP in a GAL4-based two-hybrid yeast system for screening a Nicotiana tabacum cDNA library. The interaction between CP and Fd I was confirmed by in vitro binding and bimolecular fluorescence complementation assays in plant cells.
authors.affiliation: Chongqing Key Laboratory of Plant Disease Biology, College of Plant Protection, Southwest University, Chongqing, 400715, People's Republic of China, xianchaosun@gmail.com.
authors.forename: Xianchao
authors.initials: X
authors.lastname: Sun
authors.forename: Yong
authors.initials: Y
authors.lastname: Li
authors.forename: Mengdie
authors.initials: M
authors.lastname: Shi
authors.forename: Ning
authors.initials: N
authors.lastname: Zhang
authors.forename: Gaixia
authors.initials: G
authors.lastname: Wu
authors.forename: Tingting
authors.initials: T
authors.lastname: Li
authors.forename: Ling
authors.initials: L
authors.lastname: Qing
authors.forename: Changyong
authors.initials: C
authors.lastname: Zhou
mesh_terms: D036022:Capsid Proteins / Q000378:metabolism*; D060365:Chloroplast Proteins / Q000378:metabolism*; D005288:Ferredoxins / Q000378:metabolism*; D054884:Host-Pathogen Interactions; D008967:Molecular Biology / Q000379:methods; D010935:Plant Diseases / Q000821:virology; D011485:Protein Binding; D025941:Protein Interaction Mapping; D014026:Nicotiana / Q000821:virology*; D017898:Tobamovirus / Q000502:physiology*; D020798:Two-Hybrid System Techniques; D014773:Virology / Q000379:methods
chemical_list: D036022:Capsid Proteins; D060365:Chloroplast Proteins; D005288:Ferredoxins