title: Saliva as a source of anti-Toxoplasma gondii IgG for enzyme immunoassay in human samples.
abstract: Toxoplasmosis, a highly prevalent disease, is mainly diagnosed by serology. Incidence studies could be feasible in children, but ethical concerns restrict blood sampling in this group. Saliva contains small amounts of crevicular fluid IgG. Dot-ELISA and a protein A IgG capture immunoassay were standardized for anti-Toxoplasma gondii IgG in paired saliva and serum samples from 20 adult volunteers. A frequency of toxoplasmosis of 19% (95% CI 12-28) was found in 100 saliva samples from university graduates using both assays. Toxoplasmosis immunoassays using saliva IgG are a promising tool for the investigation of the epidemiology of this disease in children and other vulnerable groups.
authors.affiliation: Laboratório de Protozoologia, Instituto de Medicina Tropical de São Paulo, São Paulo, Brazil.
authors.forename: B F C
authors.initials: BF
authors.lastname: Sampaio
authors.forename: M S
authors.initials: MS
authors.lastname: Macre
authors.forename: L R
authors.initials: LR
authors.lastname: Meireles
authors.forename: H F
authors.initials: HF
authors.lastname: Andrade
mesh_terms: D000293:Adolescent; D000913:Antibodies, Protozoan / Q000032:analysis* / Q000097:blood; D004797:Enzyme-Linked Immunosorbent Assay / Q000191:economics; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis* / Q000097:blood; D012463:Saliva / Q000276:immunology*; D012680:Sensitivity and Specificity; D016036:Seroepidemiologic Studies; D014122:Toxoplasma / Q000276:immunology*; D014123:Toxoplasmosis / Q000453:epidemiology* / Q000276:immunology; D055815:Young Adult
chemical_list: D000913:Antibodies, Protozoan; D007074:Immunoglobulin G
keywords: Dot-ELISA; ELISA; IgG; immunoassay; protein A; saliva; toxoplasma; toxoplasmosis