title: Characterization of the enzymatic activity of Clostridium perfringens TpeL.
abstract: TpeL is a toxin produced by Clostridium perfringens which belongs to the large clostridial glucosylating toxin family. It was shown that TpeL modifies Ras using UDP-glucose or UDP-N-acetylglucosamine as cosubstrates (Guttenberg et al., 2012; Nagahama et al., 2011). We confirmed that TpeL preferentially glucosaminates the three isoforms of Ras (cH-Ras, N-Ras, and K-Ras) from UDP-N-acetylglucosamine and to a lower extent Rap1a and R-Ras3, and very weakly Rac1. In contrast to previous report, we observed that Ral was not a substrate of TpeL. In addition, we confirmed by in vitro glucosylation and mass spectrometry that TpeL modifies cH-Ras at Thr35.
authors.affiliation: Institut Pasteur, Unité des Bactéries anaérobies et Toxines, 25 rue du Dr Roux, 75724 Paris Cedex 15, France.
authors.forename: Serge
authors.initials: S
authors.lastname: Pauillac
authors.forename: Jacques
authors.initials: J
authors.lastname: D'allayer
authors.forename: Pascal
authors.initials: P
authors.lastname: Lenormand
authors.forename: Jean Claude
authors.initials: JC
authors.lastname: Rousselle
authors.forename: Philippe
authors.initials: P
authors.lastname: Bouvet
authors.forename: Michel R
authors.initials: MR
authors.lastname: Popoff
mesh_terms: D001427:Bacterial Toxins / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D003016:Clostridium perfringens / Q000201:enzymology* / Q000235:genetics; D013379:Substrate Specificity; D053719:Tandem Mass Spectrometry; D014537:Uridine Diphosphate N-Acetylglucosamine / Q000378:metabolism; D020744:rac GTP-Binding Proteins / Q000378:metabolism; D018631:ras Proteins / Q000378:metabolism
chemical_list: D001427:Bacterial Toxins; D014537:Uridine Diphosphate N-Acetylglucosamine; D020744:rac GTP-Binding Proteins; D018631:ras Proteins
keywords: Clostridium perfringens; Rac; Ras; Rho-GTPases; Toxin; TpeL