title: A census of RND superfamily proteins in the Burkholderia genus.
abstract: OBJECTIVE
  The aim of this work was to analyze the eight resistance-nodulation-cell division (RND) families (a group of proteins mainly involved in multidrug resistance of Gram-negative bacteria) in 26 Burkholderia genomes in order to gain knowledge regarding their presence and distribution, to obtain a platform for future experimental tests aimed to identify new molecular targets to be used in antimicrobial therapy against Burkholderia species and to refine the annotation of RND-like sequences in these genomes.
  
  
  METHODS
  A total of 417 coding sequences were retrieved and analyzed using different bioinformatics tools.
  
  
  CONCLUSIONS
  A complex pattern of RND presence and distribution in the different Burkholderia species was disclosed and a core of proteins represented in all 26 genomes was identified. These 'core' proteins might represent useful targets of new synthetic antimicrobial compounds. Furthermore, the annotation of RND-like sequences in Burkholderia was refined.
authors.affiliation: Laboratory of Molecular & Microbial Evolution, Department of Biology, University of Florence, Via Madonna del Piano 6, 50019 Sesto Fiorentino (Fi), Italy.
authors.forename: Elena
authors.initials: E
authors.lastname: Perrin
authors.forename: Marco
authors.initials: M
authors.lastname: Fondi
authors.forename: Maria Cristiana
authors.initials: MC
authors.lastname: Papaleo
authors.forename: Isabel
authors.initials: I
authors.lastname: Maida
authors.forename: Giovanni
authors.initials: G
authors.lastname: Emiliani
authors.forename: Silvia
authors.initials: S
authors.lastname: Buroni
authors.forename: Maria Rosalia
authors.initials: MR
authors.lastname: Pasca
authors.forename: Giovanna
authors.initials: G
authors.lastname: Riccardi
authors.forename: Renato
authors.initials: R
authors.lastname: Fani
mesh_terms: D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D019117:Burkholderia / Q000145:classification / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D019295:Computational Biology; D024901:Drug Resistance, Multiple, Bacterial; D026901:Membrane Transport Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D005810:Multigene Family; D009876:Operon; D010802:Phylogeny; D017434:Protein Structure, Tertiary; D016415:Sequence Alignment
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D026901:Membrane Transport Proteins