title: Isolation of cytotoxic compounds from the seeds of Crataegus pinnatifida.
abstract: OBJECTIVE
  To study the chemical constituents and bioactivity of the seeds of Crataegus pinnatifida.
  
  
  METHODS
  The chemical constituents were isolated and purified by macroporous adsorptive resin D101, silica gel, and ODS column chromatography, and preparative HPLC. Their structures were elucidated on the basis of spectroscopic methods. In addition, the cytotoxic activities of compounds 1-4 were investigated on OPM2 and RPMI-8226 cells.
  
  
  RESULTS
  Four compounds were obtained and their structures were identified as (7S, 8S)-4-[2-hydroxy-2-(4-hydroxy-3-methoxyphenyl)-1-(hydroxymethyl)ethoxy]-3, 5-dimethoxybenzaldehyde (1), (+)-balanophonin (2), erythro-guaiacylglycerol-β-coniferyl aldehyde ether (3), buddlenol A (4).
  
  
  CONCLUSIONS
  Compound 1 is a novel norlignan, while compounds 1-4 exhibited marginal inhibition on the proliferation of OPM2 and RPMI-8226 cells.
authors.affiliation: School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, China.
authors.forename: Ling-Zhi
authors.initials: LZ
authors.lastname: Li
authors.forename: Ying
authors.initials: Y
authors.lastname: Peng
authors.forename: Chao
authors.initials: C
authors.lastname: Niu
authors.forename: Pin-Yi
authors.initials: PY
authors.lastname: Gao
authors.forename: Xiao-Xiao
authors.initials: XX
authors.lastname: Huang
authors.forename: Xin-Liang
authors.initials: XL
authors.lastname: Mao
authors.forename: Shao-Jiang
authors.initials: SJ
authors.lastname: Song
mesh_terms: D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D027825:Crataegus / Q000737:chemistry*; D006801:Humans; D000090023:Metallothionein 3; D015394:Molecular Structure; D009419:Nerve Tissue Proteins / Q000737:chemistry / Q000302:isolation & purification* / Q000633:toxicity*; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification* / Q000633:toxicity*; D012639:Seeds / Q000737:chemistry*
chemical_list: D000090023:Metallothionein 3; D009419:Nerve Tissue Proteins; D010936:Plant Extracts
keywords: Crataegus pinnatifida; Cytotoxic activity; Norlignans