title: The Bfp60 surface adhesin is an extracellular matrix and plasminogen protein interacting in Bacteroides fragilis.
abstract: Plasminogen (Plg) is a highly abundant protein found in the plasma component of blood and is necessary for the degradation of fibrin, collagen, and other structural components of tissues. This fibrinolytic system is utilized by several pathogenic species of bacteria to manipulate the host plasminogen system and facilitate invasion of tissues during infection by modifying the activation of this process through the binding of Plg at their surface. Bacteroides fragilis is the most commonly isolated Gram-negative obligate anaerobe from human clinical infections, such as intra-abdominal abscesses and anaerobic bacteraemia. The ability of B. fragilis to convert plasminogen (Plg) into plasmin has been associated with an outer membrane protein named Bfp60. In this study, we characterized the function of Bfp60 protein in B. fragilis 638R by constructing the bfp60 defective strain and comparing its with that of the wild type regarding binding to laminin-1 (LMN-1) and activation of Plg into plasmin. Although the results showed in this study indicate that Bfp60 surface protein of B. fragilis is important for the recognition of LMN-1 and Plg activation, a significant slow activation of Plg into plasmin was observed in the mutant strain. For that reason, the possibility of another unidentified mechanism activating Plg is also present in B. fragilis cannot be discarded. The results demonstrate that Bfp60 protein is responsible for the recognition of laminin and Plg-plasmin activation. Although the importance of this protein is still unclear in the pathogenicity of the species, it is accepted that since other pathogenic bacteria use this mechanism to disseminate through the extracellular matrix during the infection, it should also contribute to the virulence of B. fragilis.
authors.affiliation: Laboratório de Biologia de Anaeróbios, Departamento de Microbiologia Médica, UFRJ, Ilha do Fundão, CCS, Instituto de Microbiologia Prof. Paulo de Góes, Rio de Janeiro, Brazil; Universidade Federal do Rio de Janeiro - Polo Xerém, Estrada de Xerém, 27, Duque de Caxias, Rio de Janeiro, CEP: 25245-390, Brazil. Electronic address: eliane_ferreirarj@yahoo.com.
authors.forename: Eliane de Oliveira
authors.initials: Ede O
authors.lastname: Ferreira
authors.forename: Felipe Lopes
authors.initials: FL
authors.lastname: Teixeira
authors.forename: Fabiana
authors.initials: F
authors.lastname: Cordeiro
authors.forename: Leandro
authors.initials: L
authors.lastname: Araujo Lobo
authors.forename: Edson R
authors.initials: ER
authors.lastname: Rocha
authors.forename: Jeffrey C
authors.initials: JC
authors.lastname: Smith
authors.forename: Regina M C P
authors.initials: RM
authors.lastname: Domingues
mesh_terms: D018829:Adhesins, Bacterial / Q000235:genetics / Q000378:metabolism*; D001422:Bacterial Adhesion; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D001441:Bacteroides fragilis / Q000235:genetics / Q000502:physiology*; D005341:Fibrinolysin / Q000378:metabolism; D055786:Gene Knockout Techniques; D054884:Host-Pathogen Interactions; D006801:Humans; D007797:Laminin / Q000378:metabolism*; D010958:Plasminogen / Q000378:metabolism*; D011485:Protein Binding
chemical_list: D018829:Adhesins, Bacterial; D001425:Bacterial Outer Membrane Proteins; D007797:Laminin; C063910:adhesin, Bacteroides; C121346:laminin 1; D010958:Plasminogen; D005341:Fibrinolysin
keywords: Bacteroides fragilis; Bfp60; Extracellular matrix; Plasminogen