title: Exosomes mediate the cell-to-cell transmission of IFN-α-induced antiviral activity.
abstract: The cell-to-cell transmission of viral resistance is a potential mechanism for amplifying the interferon-induced antiviral response. In this study, we report that interferon-α (IFN-α) induced the transfer of resistance to hepatitis B virus (HBV) from nonpermissive liver nonparenchymal cells (LNPCs) to permissive hepatocytes via exosomes. Exosomes from IFN-α-treated LNPCs were rich in molecules with antiviral activity. Moreover, exosomes from LNPCs were internalized by hepatocytes, which mediated the intercellular transfer of antiviral molecules. Finally, we found that exosomes also contributed to the antiviral response of IFN-α to mouse hepatitis virus A59 and adenovirus in mice. Thus, we propose an antiviral mechanism of IFN-α activity that involves the induction and intercellular transfer of antiviral molecules via exosomes.
authors.affiliation: Key Laboratory of Medical Molecular Virology, School of Basic Medical Sciences, Shanghai Medical College, Fudan University, Shanghai, China.
authors.forename: Jianhua
authors.initials: J
authors.lastname: Li
authors.forename: Kuancheng
authors.initials: K
authors.lastname: Liu
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.forename: Yan
authors.initials: Y
authors.lastname: Xu
authors.forename: Fei
authors.initials: F
authors.lastname: Zhang
authors.forename: Huijuan
authors.initials: H
authors.lastname: Yang
authors.forename: Jiangxia
authors.initials: J
authors.lastname: Liu
authors.forename: Tingting
authors.initials: T
authors.lastname: Pan
authors.forename: Jieliang
authors.initials: J
authors.lastname: Chen
authors.forename: Min
authors.initials: M
authors.lastname: Wu
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Zhou
authors.forename: Zhenghong
authors.initials: Z
authors.lastname: Yuan
mesh_terms: D000818:Animals; D055354:Exosomes / Q000276:immunology / Q000821:virology*; D056945:Hep G2 Cells; D006509:Hepatitis B / Q000188:drug therapy / Q000276:immunology*; D006515:Hepatitis B virus / Q000276:immunology*; D006801:Humans; D015151:Immunoblotting; D016898:Interferon-alpha / Q000494:pharmacology*; D008099:Liver / Q000276:immunology / Q000821:virology*; D051379:Mice; D015398:Signal Transduction / Q000276:immunology; D014779:Virus Replication / Q000276:immunology
chemical_list: D016898:Interferon-alpha