title: Genotyping analyses for polymorphisms of ANXA5 gene in patients with recurrent pregnancy loss.
abstract: OBJECTIVE
  To investigate whether polymorphisms at the promoter or 5'-untranslated region of annexin A5 gene (ANXA5) influence miscarriage.
  
  
  METHODS
  Case-control study and nested case-control study.
  
  
  METHODS
  Hospitals.
  
  
  METHODS
  A total of 264 patients with two to nine recurrent pregnancy losses (RPLs) and 195 fertile control subjects.
  
  
  METHODS
  None.
  
  
  METHODS
  The frequency of six single-nucleotide polymorphisms (SNPs) of the ANXA5 gene in RPL patients versus control subjects, and subsequent live birth rate with and without risk alleles in RPL patients.
  
  
  RESULTS
  The minor allele was significantly more frequent in RPL patients than in control subjects for SNP5 (rs1050606). The live birth rates of patients with and without risk alleles of SNP5 were 84.0% and 84.3%, respectively, after excluding cases with abnormal embryonic karyotype, with no significant difference.
  
  
  CONCLUSIONS
  The variations with the ANXA5 gene upstream region, especially SNP5, were confirmed to be risk factors of RPL. However, presence/absence of the ANXA5 risk allele did not have any predictive effect for subsequent pregnancy outcome. This was the first study indicating the influence of ANXA5 SNP5 for pregnancy outcome.
authors.affiliation: Department of Obstetrics and Gynecology, Graduate School of Medical Sciences, Nagoya City University, Nagoya, Japan.
authors.forename: Yuko
authors.initials: Y
authors.lastname: Hayashi
authors.forename: Hidefumi
authors.initials: H
authors.lastname: Sasaki
authors.forename: Sadao
authors.initials: S
authors.lastname: Suzuki
authors.forename: Takeshi
authors.initials: T
authors.lastname: Nishiyama
authors.forename: Tamao
authors.initials: T
authors.lastname: Kitaori
authors.forename: Eita
authors.initials: E
authors.lastname: Mizutani
authors.forename: Nobuhiro
authors.initials: N
authors.lastname: Suzumori
authors.forename: Mayumi
authors.initials: M
authors.lastname: Sugiura-Ogasawara
mesh_terms: D020121:5' Untranslated Regions; D000026:Abortion, Habitual / Q000235:genetics*; D000328:Adult; D017304:Annexin A5 / Q000235:genetics*; D016022:Case-Control Studies; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D006801:Humans; D050498:Live Birth; D016015:Logistic Models; D015999:Multivariate Analysis; D016017:Odds Ratio; D010641:Phenotype; D020641:Polymorphism, Single Nucleotide; D011247:Pregnancy; D018873:Pregnancy Rate; D011401:Promoter Regions, Genetic; D012307:Risk Factors
chemical_list: D020121:5' Untranslated Regions; D017304:Annexin A5
keywords: Annexin A5; cohort; recurrent pregnancy loss; single-nucleotide polymorphisms