title: Raman micro-spectroscopy: a powerful tool for the monitoring of dynamic supramolecular changes in living cells.
abstract: Cellular imaging techniques have become powerful tools in cell biology. With respect to others, the techniques based on vibrational spectroscopy present a clear advantage: the molecular composition and the modification of subcellular compartments can be obtained in label-free conditions. In fact, from the evolution of positions, intensities and line widths of Raman and infrared bands in the cell spectra, characteristic information on cellular activities can be achieved, and particularly, cellular death can be investigated. In this work we present the time evolution of the Raman spectra of single live Jurkat cells (T-lymphocyte) by looking at the high frequency part of their Raman spectra, that is the CH stretching region, around 3000cm(-1). In particular, investigation into the composition or rearrangement of CH bounds, markers of cellular membrane fatty acids, can represent an important method to study and to recognize cell death. The experimental procedure we used, together with the analysis of these high frequency vibrational bands, may represent a new, improved and advantageous approach to this kind of study.
authors.affiliation: Istituto di Biofisica, Consiglio Nazionale delle Ricerche, c/o Fondazione Bruno Kessler, Via alla Cascata 56/C, 38123 Trento, Italy; Dipartimento di Fisica, Università di Trento, Via Sommarive 14, 38050 Povo, Trento, Italy. Electronic address: silvia.caponi@cnr.it.
authors.forename: Silvia
authors.initials: S
authors.lastname: Caponi
authors.forename: Lavinia
authors.initials: L
authors.lastname: Liguori
authors.forename: Alessandra
authors.initials: A
authors.lastname: Giugliarelli
authors.forename: Maurizio
authors.initials: M
authors.lastname: Mattarelli
authors.forename: Assunta
authors.initials: A
authors.lastname: Morresi
authors.forename: Paola
authors.initials: P
authors.lastname: Sassi
authors.forename: Lorena
authors.initials: L
authors.lastname: Urbanelli
authors.forename: Carlo
authors.initials: C
authors.lastname: Musio
mesh_terms: D017209:Apoptosis; D002244:Carbon / Q000737:chemistry; D002462:Cell Membrane / Q000737:chemistry; D005227:Fatty Acids / Q000737:chemistry; D006801:Humans; D006859:Hydrogen / Q000737:chemistry; D019169:Jurkat Cells; D013059:Spectrum Analysis, Raman
chemical_list: D005227:Fatty Acids; D002244:Carbon; D006859:Hydrogen
keywords: Apoptosis process; Cellular death; Cellular imaging; Jurkat cell; Necrosis process; Raman spectroscopy