title: Lack of effect of common single nucleotide polymorphisms in leukotriene pathway genes on platelet reactivity in patients with diabetes.
abstract: The aim of the present study was to investigate the effect of genetic polymorphisms in candidate genes within the leukotriene (LT) pathway on platelet reactivity and the concentration of selected LTs in diabetic patients treated with acetylsalicylic acid (ASA). The study cohort consisted of 287 Caucasians with type 2 diabetes who had received treatment with ASA tablets (75 mg/day) for at least three months. Platelet reactivity analyses were performed using VerifyNow aspirin and PFA‑100 assays. The measured LTs included leukotriene B4 (LTB4) and leukotriene E4 (LTE4). Genotyping for the selected 25 single nucleotide polymorphisms (SNPs) within six genes of the LT pathway was performed using a Sequenom iPLEX platform. No statistically significant association was observed between the investigated SNP genotypes, platelet reactivity and measured LTs in the patient cohort. The results of our study suggest that certain polymorphisms of the LT pathway are not associated with altered platelet reactivity and the measured LTs in diabetic patients treated with ASA.
authors.affiliation: Department of Cardiology, Medical University of Warsaw, Warsaw 02‑927, Poland.
authors.forename: Marek
authors.initials: M
authors.lastname: Rosiak
authors.forename: Marek
authors.initials: M
authors.lastname: Postula
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Kaplon-Cieslicka
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Kondracka
authors.forename: Ewa
authors.initials: E
authors.lastname: Trzepla
authors.forename: Malgorzata
authors.initials: M
authors.lastname: Zaremba
authors.forename: Krzysztof J
authors.initials: KJ
authors.lastname: Filipiak
authors.forename: Dariusz A
authors.initials: DA
authors.lastname: Kosior
authors.forename: Andrzej
authors.initials: A
authors.lastname: Czlonkowski
authors.forename: Grzegorz
authors.initials: G
authors.lastname: Opolski
authors.forename: Piotr K
authors.initials: PK
authors.lastname: Janicki
mesh_terms: D058927:5-Lipoxygenase-Activating Proteins / Q000235:genetics*; D000368:Aged; D000483:Alleles; D001092:Arachidonate 12-Lipoxygenase / Q000235:genetics*; D001093:Arachidonate 15-Lipoxygenase / Q000235:genetics*; D001094:Arachidonate 5-Lipoxygenase / Q000235:genetics*; D001241:Aspirin / Q000627:therapeutic use; D001792:Blood Platelets / Q000378:metabolism*; D015331:Cohort Studies; D003924:Diabetes Mellitus, Type 2 / Q000188:drug therapy / Q000235:genetics*; D004851:Epoxide Hydrolases / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D005982:Glutathione Transferase / Q000235:genetics*; D006801:Humans; D007975:Leukotriene B4 / Q000378:metabolism; D017999:Leukotriene E4 / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide
chemical_list: D058927:5-Lipoxygenase-Activating Proteins; C548592:ALOX5AP protein, human; D007975:Leukotriene B4; D017999:Leukotriene E4; D001092:Arachidonate 12-Lipoxygenase; C476558:ALOX12 protein, human; C515716:ALOX15 protein, human; D001093:Arachidonate 15-Lipoxygenase; D001094:Arachidonate 5-Lipoxygenase; C583212:ALOX5 protein, human; D005982:Glutathione Transferase; D004851:Epoxide Hydrolases; C041116:leukotriene-C4 synthase; D001241:Aspirin; C043134:leukotriene A4 hydrolase