title: Effects of cobalt on membrane ATPases, oxidant, and antioxidant values in the cerebrum and cerebellum of suckling rats.
abstract: Chronic overexposure to cobalt (Co) may result in neurotoxic effects, but the mechanism of Co-induced neurotoxicity is not yet well established. Our study was conducted to determine whether Co is associated to the induction of central nervous system damage in pregnant rats and their progeny. Twelve pregnant female rats were randomly divided into 2 groups: group I served as controls and group II received Co (350 mg/L, orally). Treatments started from the 14th day of pregnancy until day 14 after delivery. Co concentration in plasma was higher in the treated groups than in the controls. Exposure to Co also increased the levels of MDA, PCO, H2O2, and AOPP, while Na(+)K(+)-ATPase and Mg(2+)-ATPase, AChE, and BuChE activities decreased in the cerebrum and cerebellum of suckling pups. A smear without ladder formation on agarose gel was also shown in the cerebrum and cerebellum, indicating random DNA degradation. A reduction in GPx, SOD, CAT, GSH, NPSH, and vitamin C values was observed. The changes were confirmed by histological results. In conclusion, these data showed that the exposure of pregnant and lactating rats to Co resulted in the development of oxidative stress and the impairment of defense systems in the cerebrum and cerebellum of their suckling pups.
authors.affiliation: Animal Physiology Laboratory, Life Sciences Department, Sfax Faculty of Sciences, University of Sfax, Sfax, Tunisia.
authors.forename: Elmouldi
authors.initials: E
authors.lastname: Garoui
authors.forename: Ibtissem
authors.initials: I
authors.lastname: Ben Amara
authors.forename: Dorra
authors.initials: D
authors.lastname: Driss
authors.forename: Awatef
authors.initials: A
authors.lastname: Elwej
authors.forename: Semia Ellouze
authors.initials: SE
authors.lastname: Chaabouni
authors.forename: Tahia
authors.initials: T
authors.lastname: Boudawara
authors.forename: Najiba
authors.initials: N
authors.lastname: Zeghal
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism; D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000284:Administration, Oral; D062107:Advanced Oxidation Protein Products / Q000378:metabolism; D000818:Animals; D000833:Animals, Suckling; D000975:Antioxidants / Q000378:metabolism*; D002091:Butyrylcholinesterase / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D002531:Cerebellum / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D054022:Cerebrum / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D003035:Cobalt / Q000008:administration & dosage / Q000097:blood / Q000633:toxicity*; D053938:DNA Fragmentation / Q000187:drug effects; D005260:Female; D058851:GPI-Linked Proteins / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000378:metabolism; D008315:Malondialdehyde / Q000378:metabolism; D016877:Oxidants / Q000378:metabolism*; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects / Q000139:chemically induced / Q000378:metabolism; D050050:Protein Carbonylation / Q000187:drug effects; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D062107:Advanced Oxidation Protein Products; D000975:Antioxidants; D058851:GPI-Linked Proteins; D016877:Oxidants; D003035:Cobalt; D008315:Malondialdehyde; D006861:Hydrogen Peroxide; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D000110:Acetylcholinesterase; C548246:Ache protein, rat; D002091:Butyrylcholinesterase; D000251:Adenosine Triphosphatases