title: Gene expression profiles of metabolic aggressiveness and tumor recurrence in benign meningioma.
abstract: Around 20% of meningiomas histologically benign may be clinically aggressive and recur. This strongly affects management of meningioma patients. There is a need to evaluate the potential aggressiveness of an individual meningioma. Additional criteria for better classification of meningiomas will improve clinical decisions as well as patient follow up strategy after surgery. The aim of this study was to determine the relationship between gene expression profiles and new metabolic subgroups of benign meningioma with potential clinical relevance. Forty benign and fourteen atypical meningioma tissue samples were included in the study. We obtained metabolic profiles by NMR and recurrence after surgery information for all of them. We measured gene expression by oligonucleotide microarray measurements on 19 of them. To our knowledge, this is the first time that distinct gene expression profiles are reported for benign meningioma molecular subgroups with clinical correlation. Our results show that metabolic aggressiveness in otherwise histological benign meningioma proceeds mostly through alterations in the expression of genes involved in the regulation of transcription, mainly the LMO3 gene. Genes involved in tumor metabolism, like IGF1R, are also differentially expressed in those meningioma subgroups with higher rates of membrane turnover, higher energy demand and increased resistance to apoptosis. These new subgroups of benign meningiomas exhibit different rates of recurrence. This work shows that benign meningioma with metabolic aggressiveness constitute a subgroup of potentially recurrent tumors in which alterations in genes regulating critical features of aggressiveness, like increased angiogenesis or cell invasion, are still no predominant. The determination of these gene expression biosignatures may allow the early detection of clinically aggressive tumors.
authors.affiliation: Unidad Central de Investigación en Medicina, Universitat de Valéncia, Valencia, Spain.
authors.forename: Eva
authors.initials: E
authors.lastname: Serna
authors.forename: José Manuel
authors.initials: JM
authors.lastname: Morales
authors.forename: Manuel
authors.initials: M
authors.lastname: Mata
authors.forename: José
authors.initials: J
authors.lastname: Gonzalez-Darder
authors.forename: Teresa
authors.initials: T
authors.lastname: San Miguel
authors.forename: Rosario
authors.initials: R
authors.lastname: Gil-Benso
authors.forename: Concha
authors.initials: C
authors.lastname: Lopez-Gines
authors.forename: Miguel
authors.initials: M
authors.lastname: Cerda-Nicolas
authors.forename: Daniel
authors.initials: D
authors.lastname: Monleon
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics / Q000378:metabolism; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism*; D016000:Cluster Analysis; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D060588:LIM Domain Proteins / Q000235:genetics / Q000378:metabolism; D008297:Male; D008577:Meningeal Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D008579:Meningioma / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D008875:Middle Aged; D058977:Molecular Sequence Annotation; D009364:Neoplasm Recurrence, Local / Q000235:genetics / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction; D059467:Transcriptome; D015854:Up-Regulation
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D014408:Biomarkers, Tumor; D060588:LIM Domain Proteins; C502335:LMO3 protein, human