title: Effects of oral administration of probiotics from Mongolian dairy products on the Th1 immune response in mice.
abstract: We investigated 10 lactic acid bacteria strains with probiotic potential prepared from Mongolian dairy products for their ability to induce T helper type-1 (Th1) cytokine production in mouse immune cells in vitro and in vivo. Among these strains, the Lactobacillus plantarum 06CC2 strain was effective in elevating the level of interleukin (IL)-12p40 in co-culture with J774.1 cells and the levels of IL-12 and interferon (IFN)-γ in co-culture with mouse spleen cells in vitro. Oral administration of this strain augmented the gene expression of IFN-γ and IL-12p40 and enlarged the population of CD4(+), CD25(+), and CD49b(+) cells in the spleens of normal mice. It also significantly elevated the gene expression of IL-12 receptor β2 as well as IL-12p40 and IFN-γ in Peyer's patches. Thus oral administration of strain 06CC2 was effective in inducing Th1 cytokine production activating the Th1 immune response associated with intestinal immunity in normal mice.
authors.affiliation: Research Division, Minami Nihon Rakuno Kyodo Co, Ltd, Moriya, Miyakonojo, Miyazaki 885-0003, Japan.
authors.forename: Shiro
authors.initials: S
authors.lastname: Takeda
authors.forename: Satoshi
authors.initials: S
authors.lastname: Kawahara
authors.forename: Muneaki
authors.initials: M
authors.lastname: Hidaka
authors.forename: Hiroki
authors.initials: H
authors.lastname: Yoshida
authors.forename: Wataru
authors.initials: W
authors.lastname: Watanabe
authors.forename: Masahiko
authors.initials: M
authors.lastname: Takeshita
authors.forename: Yukiharu
authors.initials: Y
authors.lastname: Kikuchi
authors.forename: Dashnyam
authors.initials: D
authors.lastname: Bumbein
authors.forename: Michio
authors.initials: M
authors.lastname: Muguruma
authors.forename: Masahiko
authors.initials: M
authors.lastname: Kurokawa
mesh_terms: D000284:Administration, Oral; D000818:Animals; D002452:Cell Count; D002460:Cell Line; D016207:Cytokines / Q000096:biosynthesis; D003611:Dairy Products / Q000382:microbiology*; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects / Q000276:immunology; D007422:Intestines / Q000276:immunology / Q000382:microbiology; D007778:Lactobacillus / Q000502:physiology; D051379:Mice; D008986:Mongolia; D010581:Peyer's Patches / Q000276:immunology / Q000382:microbiology; D019936:Probiotics / Q000008:administration & dosage* / Q000494:pharmacology*; D011948:Receptors, Antigen, T-Cell / Q000235:genetics; D013154:Spleen / Q000276:immunology; D018417:Th1 Cells / Q000166:cytology / Q000187:drug effects* / Q000276:immunology* / Q000378:metabolism
chemical_list: D016207:Cytokines; D011948:Receptors, Antigen, T-Cell