title: Effects of exogenous zinc on cell cycle, apoptosis and viability of MDAMB231, HepG2 and 293 T cells.
abstract: As a non-toxic metal to humans, zinc is essential for cell proliferation, differentiation, regulation of DNA synthesis, genomic stability and mitosis. Zinc homeostasis in cells, which is crucial for normal cellular functioning, is maintained by various protein families including ZnT (zinc transporter/SLC30A) and ZIP (Zrt-, Irt-like proteins/SLC39A) that decrease and increase cytosolic zinc availability, respectively. In this study, we investigated the influences of a specific concentration range of ZnSO4 on cell cycle and apoptosis by flow cytometry, and cell viability by MTT method in MDAMB231, HepG2 and 293 T cell lines. Fluorescent sensors NBD-TPEA and the counterstain for nuclei Hoechst 33342 were used to stain the treated cells for observing the localisation and amount of Zn(2+) via laser scanning confocal microscope. It was found that the influence manners of ZnSO4 on cell cycle, apoptosis and cell viability in various cell lines were different and corresponding to the changes of Zn(2+) content of the three cell lines, respectively. The significant increase on intracelluar zinc content of MDAMB231 cells resulted in cell death, G1 and G2/M cell cycle arrest and increased apoptotic fraction. Additionally, the mRNA expression levels of ZnT and ZIP families in the three cell lines, when treated with high concentration of ZnSO4, increased and decreased corresponding to their functions, respectively.
authors.affiliation: State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering and Center of Materials Analysis, Nanjing University, Nanjing, China.
authors.forename: Yan-hong
authors.initials: YH
authors.lastname: Wang
authors.forename: Ke-jin
authors.initials: KJ
authors.lastname: Li
authors.forename: Li
authors.initials: L
authors.lastname: Mao
authors.forename: Xin
authors.initials: X
authors.lastname: Hu
authors.forename: Wen-jie
authors.initials: WJ
authors.lastname: Zhao
authors.forename: An
authors.initials: A
authors.lastname: Hu
authors.forename: Hong-zhen
authors.initials: HZ
authors.lastname: Lian
authors.forename: Wei-juan
authors.initials: WJ
authors.lastname: Zheng
mesh_terms: D017209:Apoptosis / Q000187:drug effects* / Q000235:genetics; D027682:Cation Transport Proteins / Q000235:genetics; D002453:Cell Cycle / Q000187:drug effects* / Q000235:genetics; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects / Q000235:genetics; D004305:Dose-Response Relationship, Drug; D005434:Flow Cytometry; D059585:G1 Phase Cell Cycle Checkpoints / Q000187:drug effects / Q000235:genetics; D059565:G2 Phase Cell Cycle Checkpoints / Q000187:drug effects / Q000235:genetics; D015870:Gene Expression / Q000187:drug effects; D057809:HEK293 Cells; D056945:Hep G2 Cells; D006801:Humans; D018613:Microscopy, Confocal; D020033:Protein Isoforms / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015032:Zinc / Q000378:metabolism / Q000494:pharmacology*; D019287:Zinc Sulfate / Q000378:metabolism / Q000494:pharmacology
chemical_list: D027682:Cation Transport Proteins; D020033:Protein Isoforms; C498299:SLC30A1 protein, human; C403517:SLC39A1 protein, human; D019287:Zinc Sulfate; D015032:Zinc