title: [HSV-1 based vector mediated IL-1Rα gene for knee osteoarthritis in rabbits].
abstract: OBJECTIVE
  To investigate the effect and mechanism of herpes simplex virus type 1 (HSV-1) based vector mediated interlukin-1 receptor antagonist (IL-1Rα) gene for knee osteoarthritis in rabbits.
  
  
  METHODS
  HSV-1 vectors containing IL-1Rα genes were constructed and injected into the joint space of the osteoarthritis knee in rabbits for 4 weeks. The rabbits were sacrificed, and the knees were lavaged, dissected and the effect of transgene expression was analyzed. Levels of IL-1Rα and IL-1 expression in the recovered lavage fluids were measured with a cytokine ELISA kit. Cartilage from the lesion areas of medial femoral condyle and synovium were observed with hematoxylin and eosin (cartilage and synovium) and toluidine blue (cartilage). The blank control group was injected pHSV-LacZ vector into rabbit knees.
  
  
  RESULTS
  Intra-articular delivery of pHSV-IL-1Rα-LacZ resulted in a significant inhibition of IL-1 level and cartilage degradation compared with those in the blank control group (P<0.05).
  
  
  CONCLUSIONS
  pHSV-LacZ is an ideal vector to mediate intra-articular gene delivery in the rabbit model of osteoarthritis. Continuous intra-articular expression of IL-1Rα can treat knee osteoarthritis by inhibiting IL-1.
authors.affiliation: Department of Surgery, Changsha Medical College, Changsha, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Wu
authors.forename: Jianming
authors.initials: J
authors.lastname: Li
authors.forename: Ying
authors.initials: Y
authors.lastname: Kong
authors.forename: Ding
authors.initials: D
authors.lastname: Chen
authors.forename: Bo
authors.initials: B
authors.lastname: Liu
authors.forename: Wanchun
authors.initials: W
authors.lastname: Wang
mesh_terms: D000818:Animals; D001169:Arthritis, Experimental / Q000628:therapy; D005260:Female; D018014:Gene Transfer Techniques; D015316:Genetic Therapy / Q000379:methods*; D005822:Genetic Vectors; D018259:Herpesvirus 1, Human / Q000235:genetics*; D007270:Injections, Intra-Articular; D053590:Interleukin 1 Receptor Antagonist Protein / Q000235:genetics*; D008297:Male; D020370:Osteoarthritis, Knee / Q000628:therapy*; D011817:Rabbits
chemical_list: D053590:Interleukin 1 Receptor Antagonist Protein