title: Detection of the Helicobacter pylori CagA protein in gastric mucosa-associated lymphoid tissue lymphoma cells: clinical and biological significance.
abstract: We previously reported that CagA can be translocated into B cells in Helicobacter pylori (HP) coculture media, and the translocation appears biologically significant as activation of the relevant cellular pathways was noticed. In this study, we further explore if CagA can be detected in malignant B cells of HP-positive gastric mucosa-associated lymphoid tissue (MALT) lymphoma. Expression of CagA was evaluated by immunohistochemistry. CagA expression was further confirmed by western blot analysis. The association between CagA expression in malignant B cells and tumor response to HP eradication therapy (HPE) was evaluated in 64 stage IE gastric MALT lymphoma patients. We detected CagA expression in 31 (48.4%) of 64 patients: 26 (68.4%) of the 38 HP-dependent cases and 5 (19.2%) of the 26 HP-independent cases (P<0.001). Patients with CagA expression responded to HPE quicker than those without (median time to complete remission, 3.0 vs 6.5 months, P=0.025). Our results indicated that CagA can be translocated into malignant B cells of MALT lymphoma, and the translocation is clinically and biologically significant.
authors.affiliation: 1] Department of Oncology, National Taiwan University Hospital and National Taiwan University College of Medicine, Taipei, Taiwan [2] Department of Internal Medicine, National Taiwan University Hospital and National Taiwan University College of Medicine, Taipei, Taiwan [3] Cancer Research Center, National Taiwan University College of Medicine, Taipei, Taiwan [4] Graduate Institute of Oncology, National Taiwan University College of Medicine, Taipei, Taiwan.
authors.forename: S-H
authors.initials: SH
authors.lastname: Kuo
authors.forename: L-T
authors.initials: LT
authors.lastname: Chen
authors.forename: C-W
authors.initials: CW
authors.lastname: Lin
authors.forename: M-S
authors.initials: MS
authors.lastname: Wu
authors.forename: P-N
authors.initials: PN
authors.lastname: Hsu
authors.forename: H-J
authors.initials: HJ
authors.lastname: Tsai
authors.forename: C-Y
authors.initials: CY
authors.lastname: Chu
authors.forename: Y-S
authors.initials: YS
authors.lastname: Tzeng
authors.forename: H-P
authors.initials: HP
authors.lastname: Wang
authors.forename: K-H
authors.initials: KH
authors.lastname: Yeh
authors.forename: A-L
authors.initials: AL
authors.lastname: Cheng