title: Transcriptome sequencing of sea cucumber (Apostichopus japonicus) and the identification of gene-associated markers.
abstract: Sea cucumber (Apostichopus japonicus) is an ecologically and economically important species in East and South-East Asia. This project aimed to identify large numbers of gene-associated markers and differentially expressed genes (DEGs) after lipopolysaccharides (LPS) challenge in A. japonicus using high-throughput transcriptome sequencing. A total of 162 million high-quality reads of 174 million raw reads were obtained by deep sequencing using Illumina HiSeq™ 2000 platform. Assembly of these reads generated 94 704 unigenes, with read length ranging from 200 to 16 153 bp (average length of 810 bp). A total of 36 005 were identified as coding sequences (CDSs), 32 479 of which were successfully annotated. Based on the assembly transcriptome, we identified 142 511 high-quality single nucleotide polymorphisms (SNPs). Among them, 33 775, 63 120 and 45 616 were located in sequences without predicted CDS (non-CDSs), CDSs and untranslated regions (UTRs), respectively. These putative SNPs included 82 664 transitions and 59 847 transversions. Totally, 89 375 (59.1%) were distributed in 15 473 known genes. A total of 6417 microsatellites were detected in 5970 unigenes, 3216 of which were annotated and 2481 were successfully subjected for primer design. The numbers of simple sequence repeats (SSRs) identified in non-CDSs, CDSs and UTRs were 2367, 2316 and 1734. These potential SNPs and SSRs are expected to provide abundant resources for genetic, evolutionary and ecological studies in sea cucumber. Transcriptome comparison revealed 1330, 1347 and 1291 DEGs in the coelomocytes of A. japonicus at 4 h, 24 h and 72 h after LPS challenge, respectively. Approximately 58.4% (1802) of total DEGs have been successfully annotated.
authors.affiliation: Liaoning Key Lab of Marine Fishery Molecular Biology, Liaoning Ocean and Fisheries Science Research Institute, Dalian, Liaoning, 116023, China.
authors.forename: Z C
authors.initials: ZC
authors.lastname: Zhou
authors.forename: Y
authors.initials: Y
authors.lastname: Dong
authors.forename: H J
authors.initials: HJ
authors.lastname: Sun
authors.forename: A F
authors.initials: AF
authors.lastname: Yang
authors.forename: Z
authors.initials: Z
authors.lastname: Chen
authors.forename: S
authors.initials: S
authors.lastname: Gao
authors.forename: J W
authors.initials: JW
authors.lastname: Jiang
authors.forename: X Y
authors.initials: XY
authors.lastname: Guan
authors.forename: B
authors.initials: B
authors.lastname: Jiang
authors.forename: B
authors.initials: B
authors.lastname: Wang
mesh_terms: D000818:Animals; D001210:Asia, Southeastern; D019295:Computational Biology; D005819:Genetic Markers; D059014:High-Throughput Nucleotide Sequencing; D008070:Lipopolysaccharides / Q000633:toxicity*; D018895:Microsatellite Repeats; D008969:Molecular Sequence Data; D020641:Polymorphism, Single Nucleotide; D012616:Sea Cucumbers / Q000187:drug effects* / Q000235:genetics*; D013312:Stress, Physiological; D059467:Transcriptome
chemical_list: D005819:Genetic Markers; D008070:Lipopolysaccharides
keywords: markers development; sea cucumber (Apostichopus japonicus); transcriptome sequencing