title: Identification and genomic characterization of a novel fish reovirus, Hubei grass carp disease reovirus, isolated in 2009 in China.
abstract: A novel fish reovirus, Hubei grass carp disease reovirus (HGDRV; formerly grass carp reovirus strain 104, GCRV104), was isolated from diseased grass carp in China in 2009 and the full genome sequence was determined. This reovirus was propagated in a grass carp kidney cell line with a typical cytopathic effect. The total size of the genome was 23 706 bp with a 51 mol% G+C content, and the 11 dsRNA segments encoded 12 proteins (two proteins encoded by segment 11). A nucleotide sequence similarity search using blastn found no significant matches except for segment 2, which partially matched that of the RNA-dependent RNA polymerase (RdRp) from several viruses in the genera Aquareovirus and Orthoreovirus of the family Reoviridae. At the amino acid level, seven segments (Seg-1 to Seg-6, and Seg-8) matched with species in the genera Aquareovirus (15-46 % identities) and Orthoreovirus (12-44 % identities), while for four segments (Seg-7, Seg-9, Seg-10 and Seg-11) no similarities in these genera were found. Conserved terminal sequences, 5'-GAAUU----UCAUC-3', were found in each HGDRV segment at the 5' and 3' ends, and the 5'-terminal nucleotides were different from any known species in the genus Aquareovirus. Phylogenetic analysis based on RdRp amino acid sequences from members of the family Reoviridae showed that HGDRV clustered with aquareoviruses prior to joining a branch common with orthoreoviruses. Based on these observations, we propose that HGDRV is a new species in the genus Aquareovirus that is distantly related to any known species within this genus.
authors.affiliation: Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, PR China.|Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, PR China.
authors.forename: Yuding
authors.initials: Y
authors.lastname: Fan
authors.affiliation: Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA.
authors.forename: Shujing
authors.initials: S
authors.lastname: Rao
authors.affiliation: Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, PR China.
authors.forename: Lingbing
authors.initials: L
authors.lastname: Zeng
authors.affiliation: Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, PR China.
authors.forename: Jie
authors.initials: J
authors.lastname: Ma
authors.affiliation: Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, PR China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, PR China.
authors.forename: Jin
authors.initials: J
authors.lastname: Xu
authors.affiliation: Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, PR China.
authors.forename: Hui
authors.initials: H
authors.lastname: Zhang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002347:Carps / Q000821:virology*; D002460:Cell Line; D002681:China / Q000453:epidemiology; D004279:DNA, Viral / Q000737:chemistry; D005393:Fish Diseases / Q000453:epidemiology / Q000473:pathology / Q000821:virology*; D016679:Genome, Viral; D008969:Molecular Sequence Data; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics / Q000302:isolation & purification; D012087:Reoviridae / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D012088:Reoviridae Infections / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics
chemical_list: D004279:DNA, Viral; D012367:RNA, Viral; D014764:Viral Proteins