title: Ethanol production from glucose and xylose by immobilized Thermoanaerobacter pentosaceus at 70 °C in an up-flow anaerobic sludge blanket (UASB) reactor.
abstract: The newly isolated extreme thermophilic ethanologen Thermoanaerobacter pentosaceus was immobilized in different support materials in order to improve its ethanol production ability. In batch fermentation, a maximum ethanol yield of 1.36 mol mol(-1) consumed sugars was obtained by T. pentosaceus immobilized on rapeseed straw. Additionally, immobilized T. pentosaceus' ethanol production was improved by 11% in comparison to free cells. In continuous mode, it was shown that hydraulic retention time (HRT) affected ethanol yield, and a dramatic shift from ethanol to acetate and lactate production occurred at an HRT of 6 h. The maximum ethanol yield and concentration, 1.50 mol mol(-1) consumed sugars and 12.4 g l(-1), were obtained with an HRT of 12 h. The latter represented an improvement of 60% in relation to previously obtained results. This indicates that immobilization of T. pentosaceus is an effective strategy to improve its ethanol production ability.
authors.affiliation: Department of Environmental Engineering, Technical University of Denmark, 113 Miljoevej, 2800 Kongens Lyngby, Denmark.
authors.forename: Sureewan
authors.initials: S
authors.lastname: Sittijunda
authors.forename: Ana Faria
authors.initials: AF
authors.lastname: Tomás
authors.forename: Alissara
authors.initials: A
authors.lastname: Reungsang
authors.forename: Sompong
authors.initials: S
authors.lastname: O-thong
authors.forename: Irini
authors.initials: I
authors.lastname: Angelidaki
mesh_terms: D057919:Biological Oxygen Demand Analysis; D019149:Bioreactors; D000431:Ethanol / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism*; D012722:Sewage; D046968:Thermoanaerobacter / Q000378:metabolism*; D014994:Xylose / Q000378:metabolism*
chemical_list: D012722:Sewage; D000431:Ethanol; D014994:Xylose; D005947:Glucose
keywords: BA; Bioethanol; CDW; COD; Extreme thermophilic; GC; HRT; Immobilization; Second-generation; Thermoanaerobacter pentosaceus; basic anaerobic; cell dry weight; chemical oxygen demand; gas chromatography; hydraulic retention time