title: Serum concentrations of A disintegrin and metalloproteinase 9 (ADAM9) mRNA as a promising novel marker for the detection of pulmonary sarcoidosis.
abstract: OBJECTIVE
  This prospective study investigated the potential role of serum A disintegrin and metalloproteinase 9 (ADAM9) mRNA in the differential diagnosis of pulmonary sarcoidosis from other diseases with similar clinical presentations, such as tuberculosis (TB).
  
  
  METHODS
  Serum samples were collected from patients with sarcoidosis, and compared with those from patients with TB and healthy control subjects. Real-time quantitative reverse transcription-polymerase chain reaction was performed to measure the concentration of ADAM9 mRNA relative to the internal control, glyceraldehyde-3-phosphate dehydrogenase.
  
  
  RESULTS
  A total of 100 patients with sarcoidosis were compared with 50 patients with TB and 50 healthy control subjects. The serum concentration of ADAM9 mRNA in patients with sarcoidosis was significantly lower than that in healthy control subjects and patients with TB. The serum concentration of ADAM9 mRNA in patients with TB was significantly higher than that in healthy control subjects.
  
  
  CONCLUSIONS
  These data suggest that serum ADAM9 mRNA might be a potential biomarker for the differential diagnosis of pulmonary sarcoidosis.
authors.affiliation: Department of Respiratory Diseases, Third Affiliated Hospital, Liaoning Medical University, Jinzhou, Liaoning Province, China. liujingyu578@yahoo.cn
authors.forename: Jingyu
authors.initials: J
authors.lastname: Liu
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhu
authors.forename: Qi
authors.initials: Q
authors.lastname: Pei
authors.forename: Jinan
authors.initials: J
authors.lastname: Di
authors.forename: Suwen
authors.initials: S
authors.lastname: Zhang
mesh_terms: D051722:ADAM Proteins / Q000097:blood / Q000235:genetics*; D000328:Adult; D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D003937:Diagnosis, Differential; D005260:Female; D015870:Gene Expression; D024602:Glyceraldehyde-3-Phosphate Dehydrogenase (NADP+)(Phosphorylating) / Q000097:blood / Q000235:genetics; D006801:Humans; D008297:Male; D008565:Membrane Proteins / Q000097:blood / Q000235:genetics*; D012333:RNA, Messenger / Q000097:blood / Q000235:genetics*; D012372:ROC Curve; D017565:Sarcoidosis, Pulmonary / Q000175:diagnosis* / Q000235:genetics*; D014397:Tuberculosis, Pulmonary / Q000175:diagnosis / Q000235:genetics*
chemical_list: D015415:Biomarkers; D008565:Membrane Proteins; D012333:RNA, Messenger; D024602:Glyceraldehyde-3-Phosphate Dehydrogenase (NADP+)(Phosphorylating); D051722:ADAM Proteins; C098339:ADAM9 protein, human
keywords: A disintegrin and metalloproteinase 9; ADAM9; Pulmonary sarcoidosis; quantitative reverse transcription–polymerase chain reaction; serum concentration