title: A new diamond biosensor with integrated graphitic microchannels for detecting quantal exocytic events from chromaffin cells.
abstract: An MeV ion-microbeam lithographic technique can be successfully employed for the fabrication of an all-carbon miniaturized cellular biosensor based on graphitic microchannels embedded in a single-crystal diamond matrix. The device is functionally characterized for the in vitro recording of quantal exocytic events from single chromaffin cells, with high sensitivity and signal-to-noise ratio, opening promising perspectives for the realization of monolithic all-carbon cellular biosensors.
authors.affiliation: Department of Physics, NIS Centre of Excellence, CNISM Research Unit - University of Torino, INFN Sez. Torino, via P. Giuria 1, Torino, 10125, Italy. picollo@to.infn.it.
authors.forename: Federico
authors.initials: F
authors.lastname: Picollo
authors.forename: Sara
authors.initials: S
authors.lastname: Gosso
authors.forename: Ettore
authors.initials: E
authors.lastname: Vittone
authors.forename: Alberto
authors.initials: A
authors.lastname: Pasquarelli
authors.forename: Emilio
authors.initials: E
authors.lastname: Carbone
authors.forename: Paolo
authors.initials: P
authors.lastname: Olivero
authors.forename: Valentina
authors.initials: V
authors.lastname: Carabelli
mesh_terms: D000311:Adrenal Glands / Q000166:cytology; D000818:Animals; D015374:Biosensing Techniques; D002478:Cells, Cultured; D019439:Chromaffin Cells / Q000166:cytology* / Q000378:metabolism; D018130:Diamond / Q000737:chemistry*; D055664:Electrochemical Techniques; D005089:Exocytosis / Q000502:physiology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008839:Microelectrodes; D008904:Miniaturization; D059629:Signal-To-Noise Ratio
chemical_list: D018130:Diamond
keywords: cellular biosensors; exocytosis; ion beam lithography; single-crystal diamond