title: [Preliminary study on the discrimination of oral mucosa pathogens Saccharomyces with metabonomics method].
abstract: OBJECTIVE
  To evaluate the feasibility of identifying oral mucosa pathogenic bacteria by comparing the metabolic profiling of Saccharomyces and try to find a convenient and rapid way to discriminate oral micro-organisms.
  
  
  METHODS
  Saccharomyces albicans ATCC 10231 and Saccharomyces tropicalis ATCC 13803 and Saccharomyces glabrata ATCC 15126 were respectively inoculated in sabouraud medium. The growth quantity were measured periodically by a spectrophotometer. And the growth curves of the inoculated bacteria were completed. The culture solutions in stationary phases of the three bacteria were respectively tested with 'H-nuclear magnetic resonance(1H-NMR) spectroscopy. The data of 1H-NMR spectroscopy results were analyzed by principal components analysis (PCA).
  
  
  RESULTS
  The PCA showed respectively a obvious clustering phenomena and the points of every two groups' data stayed separately. Therefore, the NMR-based metabonomics profiles could discriminate the three kinds of mucosa pathogenic bacteria.
  
  
  CONCLUSIONS
  The metabonomics can be expected to be a kind of promising useful method in quick discrimination of oral pathogenic bacteria.
authors.affiliation: Dept. of Oral Medicine, School of Stomatology, Lanzhou University, Lanzhou 730000, China.
authors.forename: Miao
authors.initials: M
authors.lastname: Li
authors.forename: Xiaomin
authors.initials: X
authors.lastname: Zhang
authors.forename: Qingzong
authors.initials: Q
authors.lastname: Si
mesh_terms: D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D055432:Metabolomics; D009061:Mouth Mucosa; D012440:Saccharomyces