title: Plasma treated polyethylene grafted with adhesive molecules for enhanced adhesion and growth of fibroblasts.
abstract: The cell-material interface plays a crucial role in the interaction of cells with synthetic materials for biomedical use. The application of plasma for tailoring polymer surfaces is of abiding interest and holds a great promise in biomedicine. In this paper, we describe polyethylene (PE) surface tuning by Ar plasma irradiating and subsequent grafting of the chemically active PE surface with adhesive proteins or motives to support cell attachment. These simple modifications resulted in changed polymer surface hydrophilicity, roughness and morphology, which we thoroughly characterized. The effect of our modifications on adhesion and growth was tested in vitro using mouse embryonic fibroblasts (NIH 3T3 cell line). We demonstrate that the plasma treatment of PE had a positive effect on the adhesion, spreading, homogeneity of distribution and moderately on proliferation activity of NIH 3T3 cells. This effect was even more pronounced on PE coated with biomolecules.
authors.affiliation: Department of Biochemistry and Microbiology, Institute of Chemical Technology, Prague, Technická 5, Prague 6, 166 28, Czech Republic.
authors.forename: Silvie
authors.initials: S
authors.lastname: Rimpelová
authors.forename: Nikola Slepičková
authors.initials: NS
authors.lastname: Kasálková
authors.forename: Petr
authors.initials: P
authors.lastname: Slepička
authors.forename: Helena
authors.initials: H
authors.lastname: Lemerová
authors.forename: Václav
authors.initials: V
authors.lastname: Švorčík
authors.forename: Tomáš
authors.initials: T
authors.lastname: Ruml
mesh_terms: D000268:Adhesiveness / Q000187:drug effects; D000818:Animals; D002244:Carbon / Q000032:analysis; D002448:Cell Adhesion / Q000187:drug effects; D002452:Cell Count; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D005347:Fibroblasts / Q000166:cytology*; D051379:Mice; D018625:Microscopy, Atomic Force; D008856:Microscopy, Fluorescence; D041681:NIH 3T3 Cells; D009584:Nitrogen / Q000032:analysis; D010100:Oxygen / Q000032:analysis; D056951:Photoelectron Spectroscopy; D058626:Plasma Gases / Q000494:pharmacology*; D020959:Polyethylene / Q000494:pharmacology*; D013997:Time Factors; D017155:Wettability / Q000187:drug effects
chemical_list: D058626:Plasma Gases; D002244:Carbon; D020959:Polyethylene; D009584:Nitrogen; D010100:Oxygen
keywords: Biomolecules grafting; Mouse embryonic fibroblasts; Plasma treatment; Polyethylene; Surface characterization