title: Polymorphisms in HLA-related genes and psoriasis heredity in patients with psoriasis.
abstract: BACKGROUND
  The aim of this study was to investigate possible associations of the five DNA polymorphic genotypes in the HLA region (transporter associated with antigen processing [TAP1; TAP1 333 a/b, TAP1 637 c/d], the HLA-DRB1*1501-rs3135388, tumor necrosis factor [TNF]α [-238 G/A] and NcoI TNFβ) with characteristics of family history in patients with psoriasis vulgaris.
  
  
  METHODS
  A total of 201 Czech patients with psoriasis were enrolled in the study. The patients were genotyped for the five common polymorphisms in TAP1, TNFα, and TNFβ genes (6p21.3) using the polymerase chain reaction-restriction fragment length polymorphism-based methodology.
  
  
  RESULTS
  We observed significantly higher prevalence of Ile333Ile TAP1 allele in patients whose first-degree relatives had a positive family history of psoriasis (Pa  = 0.04). No differences related to family history of psoriasis were observed in HLA-DRB1*1501 polymorphism. As for the TNFα (-238 G/A) polymorphism, a significant increase of the GG genotype was observed in patients, especially men with second- and third-degree relatives with psoriasis (Pg  = 0.008). Similarly, the B2B2 genotype of NcoI TNFβ polymorphism was more frequent in psoriatic patients, especially women, whose second- and third-degree relatives had psoriasis (Pg  = 0.004). Finally, the haplotype analysis of all five polymorphisms revealed that the frequency of haplotype bcCB1A was different between not only men and women with psoriasis (P = 0.007) but also between men and women without a family history of psoriasis (P = 0.007).
  
  
  CONCLUSIONS
  Haplotype association of HLA gene polymorphisms with genealogy aspects of psoriasis facilitates a better understanding of etiopathogenetic aspects of the diseases.
authors.affiliation: Department of Pathophysiology, Faculty of Medicine, Masaryk University Brno, Brno, Czech Republic.
authors.forename: Anna
authors.initials: A
authors.lastname: Vasku
authors.forename: Julie
authors.initials: J
authors.lastname: Bienertova-Vasku
authors.forename: Lydie
authors.initials: L
authors.lastname: Izakovicova-Holla
authors.forename: Monika
authors.initials: M
authors.lastname: Pavkova Goldbergova
authors.forename: Zuzana
authors.initials: Z
authors.lastname: Kozacikova
authors.forename: Zbynek
authors.initials: Z
authors.lastname: Splichal
authors.forename: Vladimir
authors.initials: V
authors.lastname: Vasku
mesh_terms: D000071181:ATP Binding Cassette Transporter, Subfamily B, Member 2; D018528:ATP-Binding Cassette Transporters / Q000235:genetics*; D000328:Adult; D000368:Aged; D005192:Family Health; D005260:Female; D020022:Genetic Predisposition to Disease / Q000453:epidemiology / Q000235:genetics; D059811:HLA-DRB1 Chains / Q000235:genetics*; D006239:Haplotypes; D006801:Humans; D008233:Lymphotoxin-alpha / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D011565:Psoriasis / Q000453:epidemiology / Q000235:genetics*; D012307:Risk Factors; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics*
chemical_list: D000071181:ATP Binding Cassette Transporter, Subfamily B, Member 2; D018528:ATP-Binding Cassette Transporters; D059811:HLA-DRB1 Chains; C489631:HLA-DRB1*15:01 antigen; D008233:Lymphotoxin-alpha; C073119:TAP1 protein, human; D014409:Tumor Necrosis Factor-alpha