title: Genetic basis for in vitro and in vivo resistance to lincosamides, streptogramins A, and pleuromutilins (LSAP phenotype) in Enterococcus faecium.
abstract: As opposed to Enterococcus faecalis, which is intrinsically resistant to lincosamides, streptogramins A, and pleuromutilins (LSAP phenotype) by production of the ABC protein Lsa(A), Enterococcus faecium is naturally susceptible. Since this phenotype may be selected for in vivo by quinupristin-dalfopristin (Q-D), the aim of this study was to investigate the molecular mechanism of acquired LSAP resistance in E. faecium. Six LSAP-resistant in vitro mutants of E. faecium HM1070 as well as three different pairs of clinical isolates (pre- and postexposure to Q-D) were studied. The full genome sequence of an in vitro mutant (E. faecium UCN90B) was determined by using 454 sequencing technology and was compared with that of the parental strain. Single-nucleotide replacement was carried out to confirm the role of this mutation. By comparative genomic analysis, a point mutation was found within a 1,503-bp gene coding for an ABC homologue showing 66% amino acid identity with Lsa(A). This mutation (C1349T) led to an amino acid substitution (Thr450Ile). An identical mutation was identified in all in vitro and in vivo resistant strains but was not present in susceptible strains. The wild-type allele was named eat(A) (for Enterococcus ABC transporter), and its mutated allelic variant was named eat(A)v. The introduction of eat(A)v from UCN90B into HM1070 conferred the LSAP phenotype, whereas that of eat(A) from HM1070 into UCN90B restored susceptibility entirely. This is the first description of the molecular mechanism of acquired LSAP resistance in E. faecium. Characterization of the biochemical mechanism of resistance and the physiological role of this ABC protein need further investigations.
authors.affiliation: CHU de Caen, Service de Microbiologie, Caen, France.
authors.forename: Christophe
authors.initials: C
authors.lastname: Isnard
authors.forename: Brigitte
authors.initials: B
authors.lastname: Malbruny
authors.forename: Roland
authors.initials: R
authors.lastname: Leclercq
authors.forename: Vincent
authors.initials: V
authors.lastname: Cattoir
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000235:genetics* / Q000378:metabolism; D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D004224:Diterpenes / Q000494:pharmacology; D024901:Drug Resistance, Multiple, Bacterial / Q000187:drug effects / Q000235:genetics*; D016984:Enterococcus faecium / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D005816:Genetic Complementation Test; D005838:Genotype; D055231:Lincosamides / Q000494:pharmacology*; D008969:Molecular Sequence Data; D010641:Phenotype; D017354:Point Mutation; D011083:Polycyclic Compounds; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D025364:Streptogramin A / Q000494:pharmacology*; D000097567:Pleuromutilins
chemical_list: D018528:ATP-Binding Cassette Transporters; D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D004224:Diterpenes; D055231:Lincosamides; D011083:Polycyclic Compounds; D025364:Streptogramin A; D000097567:Pleuromutilins