title: Association of IL-8 gene polymorphisms with non small cell lung cancer in Tunisia: A case control study.
abstract: Interleukin 8 (IL-8), is a proinflammatory chemokine, has been reported to have angiogenic activity and to be responsible for tumor-associated angiogenesis in several cancers. In this study, we aimed to study the (IL-8) gene polymorphism in relation with risk development of non small cell lung cancer in Tunisian patient. Two single nucleotide polymorphisms (-251T/A [rs4073], +781C/T [rs2227306]) of the IL-8 gene were screened in 170 patients with NSCLC and 225 healthy controls by PCR-RFLP. Significant association for the IL-8 -251T/T genotypes (P=0.004) and an increased significant frequency of IL-8 -251T allele were noted in the patient's group (P=0.0007). Clinical analysis indicated a borderline positive association of IL-8 -251T allele among adenocarcinoma patients (P=0.003). Our study indicated that IL-8 -251T allele was highly associated with large tumor size and high grade stage of NSCLC. Moreover, a significantly increased risk of NSCLC was associated with IL-8 +781C allele in patients with large tumor size (T3 and T4) (P=0.004). IL-8 mRNA expression was found highly expressed in NSCLC patients compared to healthy controls. The same higher level was even found in patients carrying IL-8 -251T/T genotype. Our results indicated that the IL-8 promoter polymorphism is associated with NSCLC risk.
authors.affiliation: Homeostasis and Cell Dysfunction Unit Research 99/UR/08-40, Faculty of Medicine, University of Tunis El Manar II, Tunis 1007, Tunisia. Electronic address: rafahlem@yahoo.fr.
authors.forename: Ahlem
authors.initials: A
authors.lastname: Rafrafi
authors.forename: Besma
authors.initials: B
authors.lastname: Chahed
authors.forename: Safa
authors.initials: S
authors.lastname: Kaabachi
authors.forename: Wajih
authors.initials: W
authors.lastname: Kaabachi
authors.forename: Haifa
authors.initials: H
authors.lastname: Maalmi
authors.forename: Kamel
authors.initials: K
authors.lastname: Hamzaoui
authors.forename: Fayçal Haj
authors.initials: FH
authors.lastname: Sassi
mesh_terms: D000483:Alleles; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics* / Q000473:pathology; D016022:Case-Control Studies; D005260:Female; D015870:Gene Expression; D056726:Genetic Association Studies; D005838:Genotype; D006801:Humans; D016209:Interleukin-8 / Q000235:genetics*; D008175:Lung Neoplasms / Q000235:genetics* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D020641:Polymorphism, Single Nucleotide; D011379:Prognosis; D011401:Promoter Regions, Genetic; D047368:Tumor Burden; D014416:Tunisia
chemical_list: D016209:Interleukin-8
keywords: CI; COPD; Chi(2) test; IL-8; NSCLC; OR; Odds ratios; PCR–RFLP; RT-PCR; SNP; TS; X(2); chronic obstructive pulmonary disease; confidence intervals; interleukin-8; mRNA; messenger ribonucleic acid; non small cell lung cancer; polymerase chain reaction–restriction fragment length polymorphism; reverse transcription-polymerase chain reaction; single nucleotide polymorphism; tobacco smoking (TS)