title: Heavy atom containing fluorescent ribonucleoside analog probe for the fluorescence detection of RNA-ligand binding.
abstract: Although numerous biophysical tools have provided effective systems to study nucleic acids, our current knowledge on how RNA structure complements its function is limited. Therefore, development of robust tools to study the structure–function relationship of RNA is highly desired. Toward this endeavor, we have developed a new ribonucleoside analog, based on a (selenophen-2-yl)pyrimidine core, which could serve as a fluorescence probe to study the function of RNA in real time and as an anomalous scattering label (selenium atom) for the phase determination in X-ray crystallography. The fluorescent selenophene-modified uridine analog is minimally perturbing and exhibits probe-like properties such as sensitivity to microenvironment and conformation changes. Utilizing these properties and amicability of the corresponding ribonucleotide analog to enzymatic incorporation, we have synthesized a fluorescent bacterial ribosomal decoding site (A-site) RNA construct and have developed a fluorescence binding assay to effectively monitor the binding of aminoglycoside antibiotics to the A-site. Our results demonstrate that this simple approach of building a dual probe could provide new avenues to study the structure–function relationship of not only nucleic acids, but also other biomacromolecules.
authors.forename: Maroti G
authors.initials: MG
authors.lastname: Pawar
authors.forename: Ashok
authors.initials: A
authors.lastname: Nuthanakanti
authors.forename: Seergazhi G
authors.initials: SG
authors.lastname: Srivatsan
mesh_terms: D000617:Aminoglycosides / Q000378:metabolism; D000900:Anti-Bacterial Agents / Q000378:metabolism; D001483:Base Sequence; D005456:Fluorescent Dyes / Q000737:chemistry*; D008024:Ligands; D016566:Organoselenium Compounds / Q000737:chemistry; D011743:Pyrimidines / Q000737:chemistry*; D012313:RNA / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D014544:Uridine Triphosphate / Q000737:chemistry
chemical_list: D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D005456:Fluorescent Dyes; D008024:Ligands; D016566:Organoselenium Compounds; D011743:Pyrimidines; D012313:RNA; C030986:pyrimidine; D014544:Uridine Triphosphate