title: [Alkaline phosphatase activity in blood group B or O secretors is fluctuated by the dinner intake of previous night].
abstract: We previously reported that two intestinal alkaline phosphatase (IAP) isoforms, high molecular mass IAP (HIAP) and normal molecular mass IAP (NIAP), appear in healthy serum with our Triton-PAGE method for determination of ALP isozymes. In addition, HIAP is chiefly present in blood group B or O secretors, and a large amount of NIAP is secreted into the circulation after high-fat meal in blood group B or O secretors. In the present paper, we investigated the relationship between alkaline phosphatase (ALP) activity in early morning with the patient in a fasted state and the dinner intake of previous night. Two types of dinner were prepared; a low-fat meal (520 kcal), and a high-fat meal (1,040 kcal). Subjects ate the 2 types of dinner on different days. The mean ALP activities at 14 h after high-fat meal ingestion in blood group B or O secretors (n=14) from JSCC and IFCC methods were 8.8% and 5.2% higher than those at 14 h after low-fat meal ingestion in blood group B or O secretors, respectively. The increases in ALP activity between after high-fat meal and low-fat meal were nearly identical to the increases in NIAP activity. These results suggest that a high-fat meal is more likely to affect ALP activity at the early morning with the patient in a fasted state in blood group B or O secretors.
authors.affiliation: Department of Clinical Chemistry, Saitama Prefectural University, Koshigaya 343-8540, Japan. matsushita-m@spu.ac.jp
authors.forename: Makoto
authors.initials: M
authors.lastname: Matsushita
authors.forename: Sanae
authors.initials: S
authors.lastname: Harajiri
authors.forename: Shiori
authors.initials: S
authors.lastname: Tabata
authors.forename: Nobuyasu
authors.initials: N
authors.lastname: Yukimasa
authors.forename: Yoshimi
authors.initials: Y
authors.lastname: Muramoto
authors.forename: Tsugikazu
authors.initials: T
authors.lastname: Komoda
mesh_terms: D000017:ABO Blood-Group System; D000469:Alkaline Phosphatase / Q000378:metabolism*; D002149:Energy Intake / Q000502:physiology*; D006801:Humans; D007527:Isoenzymes / Q000378:metabolism; D062407:Meals; D013997:Time Factors
chemical_list: D000017:ABO Blood-Group System; D007527:Isoenzymes; D000469:Alkaline Phosphatase