title: In vitro responses of neurofibroma fibroblasts, mast cells and Schwann cells obtained from patients with neurofibromatosis 1 to 308-nm excimer light and/or vitamin D3.
abstract: Fibroblasts, mast cells and Schwann cells were isolated from neurofibromas of patients with neurofibromatosis 1, and their responses to 308-nm excimer light irradiation and/or vitamin D3 or an analog (tacalcitol; 1,24-dihydroxyvitamin D3 ) were examined in vitro. Excimer light irradiation (300 mJ/cm(2) ) suppressed the growth of all three cell types. Exposure to 10(-7)  mol/L of 1α,25(OH)2 D3 (VD3 ) or tacalcitol suppressed the growth of fibroblasts and mast cells, but not Schwann cells. These results suggest that the different neurofibroma cell types show different responses to VD3 . A combination of excimer light irradiation and VD3 is necessary to suppress the growth of neurofibroma cells in vivo.
authors.affiliation: Department of Dermatology, Fukuoka University Faculty of Medicine, Fukuoka, Japan.
authors.forename: Juichiro
authors.initials: J
authors.lastname: Nakayama
authors.forename: Chiemi
authors.initials: C
authors.lastname: Sato
authors.forename: Shinichi
authors.initials: S
authors.lastname: Imafuku
mesh_terms: D002478:Cells, Cultured; D003879:Dermatologic Agents / Q000627:therapeutic use*; D004100:Dihydroxycholecalciferols / Q000627:therapeutic use*; D004354:Drug Screening Assays, Antitumor; D005347:Fibroblasts / Q000187:drug effects / Q000528:radiation effects; D006801:Humans; D054018:Lasers, Excimer / Q000627:therapeutic use*; D028022:Low-Level Light Therapy; D008407:Mast Cells / Q000187:drug effects / Q000528:radiation effects; D009456:Neurofibromatosis 1 / Q000188:drug therapy* / Q000532:radiotherapy; D012583:Schwann Cells / Q000187:drug effects / Q000528:radiation effects
chemical_list: D003879:Dermatologic Agents; D004100:Dihydroxycholecalciferols; C023850:1 alpha,24-dihydroxyvitamin D3
keywords: Schwann cells; mast cells; neurofibromatosis 1; ultraviolet light; vitamin D3