title: [Research of DNA barcode of Teseudinis Carapax et Planstrum and its adulterants based on COI gene sequence].
abstract: OBJECTIVE
  To use COI gene on the Mauremys reevesii and its adulterants by molecular identification. Search a rapid, accurate method of identification of Teseudinis Carapax et Planstrum and its adulterants.
  
  
  METHODS
  We collected 8 species of the authentic and adulterants of teseudinis carapax et planstrum in a nationwide then, extracted DNA, got the COI sequences. Use ContigExpress, Dnaman, Edit Sequence and Mega 5 to analyze the variable site and construct the N-J tree.
  
  
  RESULTS
  Compare with the authentic Teseudinis Carapax et Planstrum, the adulterant exist lots of variable site. The N-J tree Indicates that the same genus belong together and each species belong to relatively independent branch.
  
  
  CONCLUSIONS
  Based on the COI gene, the technology of DNA bar code can be a excellent identification of Teseudinis Carapax et Planstrum and its adulterants.
authors.affiliation: School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China.
authors.forename: Xiao-Fan
authors.initials: XF
authors.lastname: Liu
authors.forename: Chun-Sheng
authors.initials: CS
authors.lastname: Liu
authors.forename: Yao-Jun
authors.initials: YJ
authors.lastname: Yang
authors.forename: Jun-De
authors.initials: JD
authors.lastname: Li
authors.forename: Jia
authors.initials: J
authors.lastname: Xu
authors.forename: Li-Jie
authors.initials: LJ
authors.lastname: Wu
authors.forename: Dai
authors.initials: D
authors.lastname: Dai
authors.forename: Jie
authors.initials: J
authors.lastname: Liu
mesh_terms: D000818:Animals; D001483:Base Sequence; D058893:DNA Barcoding, Taxonomic; D003576:Electron Transport Complex IV / Q000235:genetics*; D008516:Medicine, Chinese Traditional / Q000592:standards*; D008969:Molecular Sequence Data; D010802:Phylogeny; D011786:Quality Control; D030162:Reptilian Proteins / Q000235:genetics*; D017422:Sequence Analysis, DNA; D014426:Turtles / Q000145:classification* / Q000235:genetics*
chemical_list: D030162:Reptilian Proteins; D003576:Electron Transport Complex IV