title: Microvascular anastomosis guidance and evaluation using real-time three-dimensional Fourier-domain Doppler optical coherence tomography.
abstract: Vascular and microvascular anastomoses are critical components of reconstructive microsurgery, vascular surgery, and transplant surgery. Intraoperative surgical guidance using a surgical imaging modality that provides an in-depth view and three-dimensional (3-D) imaging can potentially improve outcome following both conventional and innovative anastomosis techniques. Objective postoperative imaging of the anastomosed vessel can potentially improve the salvage rate when combined with other clinical assessment tools, such as capillary refill, temperature, blanching, and skin turgor. Compared to other contemporary postoperative monitoring modalities--computed tomography angiograms, magnetic resonance (MR) angiograms, and ultrasound Doppler--optical coherence tomography (OCT) is a noninvasive high-resolution (micron-level), high-speed, 3-D imaging modality that has been adopted widely in biomedical and clinical applications. For the first time, to the best of our knowledge, the feasibility of real-time 3-D phase-resolved Doppler OCT (PRDOCT) as an assisted intra- and postoperative imaging modality for microvascular anastomosis of rodent femoral vessels is demonstrated, which will provide new insights and a potential breakthrough to microvascular and supermicrovascular surgery.
authors.affiliation: Johns Hopkins University, Department of Electrical and Computer Engineering, 3400 N. Charles Street, Baltimore, MD 21218, USA. yhuang60@jhu.edu
authors.forename: Yong
authors.initials: Y
authors.lastname: Huang
authors.forename: Zuhaib
authors.initials: Z
authors.lastname: Ibrahim
authors.forename: Dedi
authors.initials: D
authors.lastname: Tong
authors.forename: Shan
authors.initials: S
authors.lastname: Zhu
authors.forename: Qi
authors.initials: Q
authors.lastname: Mao
authors.forename: John
authors.initials: J
authors.lastname: Pang
authors.forename: Wei Ping
authors.initials: WP
authors.lastname: Andree Lee
authors.forename: Gerald
authors.initials: G
authors.lastname: Brandacher
authors.forename: Jin U
authors.initials: JU
authors.lastname: Kang
mesh_terms: D000818:Animals; D005263:Femoral Artery / Q000601:surgery; D021621:Imaging, Three-Dimensional / Q000379:methods*; D051379:Mice; D008866:Microsurgery / Q000379:methods*; D055806:Microvessels / Q000033:anatomy & histology / Q000473:pathology / Q000601:surgery*; D051381:Rats; D011917:Rats, Inbred Lew; D025321:Surgery, Computer-Assisted / Q000379:methods*; D013927:Thrombosis / Q000473:pathology / Q000601:surgery; D041623:Tomography, Optical Coherence / Q000379:methods*