title: Alphaxalone inhibits growth, migration and invasion of rat C6 malignant glioma cells.
abstract: Malignant gliomas are the most devastating and aggressive brain tumors affecting the central nervous system. The insidious growth and infiltration are the most prominent characteristics of malignant gliomas, which render the current therapies for malignant gliomas including surgery, radiation and chemotherapy unsuccessful. Inhibition of infiltration as well as proliferation in combination with surgery might be more effective in the treatment of malignant gliomas. In the current study, we demonstrate the alphaxalone (3-hydroxypregnane-11,20-dione) could effectively inhibit the proliferation of C6 glioma cells in a concentration dependent manner. Moreover, this compound could also suppress the migration and invasion of C6 glioma cells at a concentration without causing significant cytotoxicity. Except the in vitro anti-glioma activity, alphaxalone effectively delayed the growth of rat C6 malignant glioma xenografts in vivo. Together, these findings suggest alphaxalone might be a promising candidate for the treatment of malignant gliomas and may also provide helpful clues for anti-glioma drugs development in future.
authors.affiliation: Department of Cardiology, First Affiliated Hospital, Sun Yat-Sen University, Guangzhou, Guangdong 510080, China.
authors.forename: Huawei
authors.initials: H
authors.lastname: Sun
authors.forename: Xiaoke
authors.initials: X
authors.lastname: Zheng
authors.forename: Yuehan
authors.initials: Y
authors.lastname: Zhou
authors.forename: Wenbo
authors.initials: W
authors.lastname: Zhu
authors.forename: Yanqiu
authors.initials: Y
authors.lastname: Ou
authors.forename: Minfeng
authors.initials: M
authors.lastname: Shu
authors.forename: Xiuren
authors.initials: X
authors.lastname: Gao
authors.forename: Tiandong
authors.initials: T
authors.lastname: Leng
authors.forename: Pengxin
authors.initials: P
authors.lastname: Qiu
authors.forename: Guangmei
authors.initials: G
authors.lastname: Yan
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D001932:Brain Neoplasms / Q000188:drug therapy* / Q000473:pathology; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D005260:Female; D005910:Glioma / Q000188:drug therapy* / Q000473:pathology; D006801:Humans; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009361:Neoplasm Invasiveness; D011277:Pregnanediones / Q000494:pharmacology*; D051381:Rats; D047368:Tumor Burden / Q000187:drug effects; D023041:Xenograft Model Antitumor Assays
chemical_list: D000970:Antineoplastic Agents; D011277:Pregnanediones; C006477:alphaxalone
keywords: Alphaxalone; Invasion; Malignant glioma; Proliferation