title: A mitochondria-specific visible-light sensitized europium β-diketonate complex with red emission.
abstract: Our recently developed Eu(3+) coordination compound, Eu(pfppd)3(tpy) [where Hpfppd = 4,4,5,5,5-pentafluoro-3-hydroxy-1-(phenanthren-3-yl)pentanedione and tpy = 2,2':6,6''-terpyridine] exhibits significant quantum yield (Φoverall = 41%) and long (5)D0 lifetime (880 μs) values under biologically relevant pH conditions (pH = 7.4) when excited with visible light. Hence, the Eu(3+) luminescent complex was examined for live cell imaging using the rat embryonic heart cell line, H9c2. The ternary Eu(3+) complex permeates into the H9c2 cells and co-localises with the mitochondria, as demonstrated by counterstaining experiments. Furthermore, the designed Eu(3+) bioprobe remains undissociated in the cell medium, showed a good cell permeability and a fast cellular uptake with a specific localization profile. Thus it has the potential to become a time-resolved imaging probe that is excitable in the visible-light range.
authors.affiliation: Chemical Sciences and Technology Division, CSIR-Network of Institutes for Solar Energy, National Institute for Interdisciplinary Science & Technology (NIIST), CSIR, Thiruvananthapuram-695 019, India.
authors.forename: V
authors.initials: V
authors.lastname: Divya
authors.forename: Vandana
authors.initials: V
authors.lastname: Sankar
authors.forename: K G
authors.initials: KG
authors.lastname: Raghu
authors.forename: M L P
authors.initials: ML
authors.lastname: Reddy
mesh_terms: D000818:Animals; D002460:Cell Line; D056831:Coordination Complexes / Q000138:chemical synthesis / Q000737:chemistry*; D005063:Europium / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D008027:Light; D008856:Microscopy, Fluorescence; D008928:Mitochondria / Q000378:metabolism*; D010616:Phenanthrenes / Q000737:chemistry; D011725:Pyridines / Q000737:chemistry; D051381:Rats
chemical_list: D056831:Coordination Complexes; D010616:Phenanthrenes; D011725:Pyridines; D005063:Europium; C031181:phenanthrene; C023666:pyridine