title: Optimization of sample preparation variables for wedelolactone from Eclipta alba using Box-Behnken experimental design followed by HPLC identification.
abstract: BACKGROUND
  Coumestan wedelolactone is an important phytocomponent from Eclipta alba (L.) Hassk. It possesses diverse pharmacological activities, which have prompted the development of various extraction techniques and strategies for its better utilization. The aim of the present study is to develop and optimize supercritical carbon dioxide assisted sample preparation and HPLC identification of wedelolactone from E. alba (L.) Hassk.
  
  
  METHODS
  The response surface methodology was employed to study the optimization of sample preparation using supercritical carbon dioxide for wedelolactone from E. alba (L.) Hassk. The optimized sample preparation involves the investigation of quantitative effects of sample preparation parameters viz. operating pressure, temperature, modifier concentration and time on yield of wedelolactone using Box-Behnken design. The wedelolactone content was determined using validated HPLC methodology. The experimental data were fitted to second-order polynomial equation using multiple regression analysis and analyzed using the appropriate statistical method.
  
  
  RESULTS
  By solving the regression equation and analyzing 3D plots, the optimum extraction conditions were found to be: extraction pressure, 25 MPa; temperature, 56 °C; modifier concentration, 9.44% and extraction time, 60 min. Optimum extraction conditions demonstrated wedelolactone yield of 15.37 ± 0.63 mg/100 g E. alba (L.) Hassk, which was in good agreement with the predicted values.
  
  
  CONCLUSIONS
  Temperature and modifier concentration showed significant effect on the wedelolactone yield. The supercritical carbon dioxide extraction showed higher selectivity than the conventional Soxhlet assisted extraction method.
authors.affiliation: Pharmaceutical Technology Division, Department of Chemical Technology, Dr. Babasaheb Ambedkar Marathwada University, Aurangabad 431004, MH, India.
authors.forename: A A
authors.initials: AA
authors.lastname: Patil
authors.forename: B S
authors.initials: BS
authors.lastname: Sachin
authors.forename: D B
authors.initials: DB
authors.lastname: Shinde
authors.forename: P S
authors.initials: PS
authors.lastname: Wakte
mesh_terms: D002138:Calibration; D002245:Carbon Dioxide / Q000737:chemistry; D005591:Chemical Fractionation; D002851:Chromatography, High Pressure Liquid; D003374:Coumarins / Q000032:analysis*; D036601:Eclipta / Q000737:chemistry*; D007202:Indicators and Reagents; D011786:Quality Control; D012044:Regression Analysis; D015203:Reproducibility of Results; D012107:Research Design
chemical_list: D003374:Coumarins; D007202:Indicators and Reagents; C051122:wedelolactone; D002245:Carbon Dioxide; C505898:coumestan
keywords: Box-Behnken conception; Box-Behnken design; Eclipta alba; Supercritical carbon dioxide extraction; Supercritique d’extraction du dioxyde de carbone; Wedelolactone