title: Advantage of Applying OSC to (1)H NMR-Based Metabonomic Data of Celiac Disease.
abstract: BACKGROUND
  Celiac disease (CD) is a disorder associated with body reaction to gluten. After the gluten intake, an immune reaction against the protein occurs and damages villi of small intestine in celiac patients gradually.
  
  
  OBJECTIVE
  The OSC, a filtering method for minimization of inter- and intra-spectrometer variations that influence on data acquisition, was applied to biofluid NMR data of CD patients.
  
  
  METHODS
  In this study, metabolites of total 56 serum samples from 12 CD patients, 15 CD patients taking gluten-free diet (GFD), and 29 healthy cases were analyzed using nuclear magnetic resonance (NMR) and associated theoretical analysis. Employing ProMetab (version ProMetab_v3_3) software, data obtained from NMR spectra were reduced and orthogonal signal correction (OSC) effect on celiac disease metabonomics before and after the separation by principle component analysis (PCA) was investigated.
  
  
  RESULTS
  The three groups were separated by OSC and findings were analyzed by partial least squares discriminant analysis (PLS-DA) method. Root mean square error of calibration (RMSEc) and correlation coefficient of calibration (Rc) for PLS-DA referred to an efficient group separation filtered by OSC.
  
  
  CONCLUSIONS
  The applied leave-one-out cross-validation to PLS-DA method performed along with OSC confirmed validation of data analysis. Finally four metabolites are introduced as CD biomarkers.
authors.affiliation: Proteomics Research Center, Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, IR Iran.
authors.forename: Mostafa
authors.initials: M
authors.lastname: Rezaei-Tavirani
authors.forename: Fariba
authors.initials: F
authors.lastname: Fathi
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Darvizeh
authors.forename: Mohamad Reza
authors.initials: MR
authors.lastname: Zali
authors.forename: Mohamad
authors.initials: M
authors.lastname: Rostami Nejad
authors.forename: Kamran
authors.initials: K
authors.lastname: Rostami
authors.forename: Mohsen
authors.initials: M
authors.lastname: Tafazzoli
authors.forename: Afsaneh
authors.initials: A
authors.lastname: Arefi Oskouie
authors.forename: Seyed Abdolreza
authors.initials: SA
authors.lastname: Mortazavi-Tabatabaei
keywords: Celiac Disease; Discriminant Analysis; Magnetic Resonance Spectroscopy; Principle Component Analysis