title: Estrone specific molecularly imprinted polymeric nanospheres: synthesis, characterization and applications for electrochemical sensor development.
abstract: The aim of this study is (i) to prepare estrone-imprinted nanospheres (nano-EST-MIPs) and (ii) to integrate them into the electrochemical sensor as a recognition layer. N-methacryloyl-(l)-phenylalanine (MAPA) was chosen as the complexing monomer. Firstly, estrone (EST) was complexed with MAPA and the EST-imprinted poly(2-hyroxyethylmethacrylate-co-N-methacryloyl-(l)-phenylalanine) [EST-imprinted poly(HEMA-MAPA)] nanospheres were synthesized by surfactant- free emulsion polymerization method. The specific surface area of the EST-imprinted poly(HEMA-MAPA) nanospheres was found to be 1275 m2/g with a size of 163.2 nm in diameter. According to the elemental analysis results, the nanospheres contained 95.3 mmole MAPA/g nanosphere. The application of EST specific MIP nanospheres for the development of an electrochemical biosensor was introduced for the first time in our study by using electrochemical impedance spectroscopy (EIS) technique. This nano-MIP based sensor presented a great specificity and selectivity for EST.
authors.affiliation: Department of Analytical Chemistry, Faculty of Pharmacy, Ege University, Bornova, 35100 Izmir, Turkey.
authors.forename: Gulsah
authors.initials: G
authors.lastname: Congur
authors.forename: Hilal
authors.initials: H
authors.lastname: Senay
authors.forename: Ceren
authors.initials: C
authors.lastname: Turkcan
authors.forename: Ece
authors.initials: E
authors.lastname: Canavar
authors.forename: Arzum
authors.initials: A
authors.lastname: Erdem
authors.forename: Sinan
authors.initials: S
authors.lastname: Akgol
mesh_terms: D055664:Electrochemical Techniques / Q000295:instrumentation*; D004970:Estrone / Q000378:metabolism*; D008855:Microscopy, Electron, Scanning; D054802:Molecular Imprinting; D054118:Nanospheres; D011108:Polymers / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D011108:Polymers; D004970:Estrone