title: Novel Phenotypic Fluorescent Three-Dimensional Platforms for High-throughput Drug Screening and Personalized Chemotherapy.
abstract: We have developed novel phenotypic fluorescent three-dimensional co-culture platforms that efficiently and economically screen anti-angiogenic/anti-metastatic drugs on a high-throughput scale. Individual cell populations can be identified and isolated for protein/gene expression profiling studies and cellular movement/interactions can be tracked by time-lapse cinematography. More importantly, these platforms closely parallel the in vivo angiogenic and metastatic outcomes of a given tumor xenograft in the nude mouse model but, unlike in vivo models, our co-culture platforms produce comparable results in five to nine days. Potentially, by incorporating cancer patient biopsies, the co-culture platforms should greatly improve the effectiveness and efficiency of personalized chemotherapy.
authors.affiliation: 1. Advanced Personalized Diagnostics LLC, 6006 Bangor Drive, Alexandria, VA 22303, USA. ; 2. Angiogenesis Core Facility, Advanced Technology Center, Room 115, Center for Cancer Research, National Cancer Institute, 8717 Grovemont Circle, Bethesda, MD 20892-4605, USA.
authors.forename: Changge
authors.initials: C
authors.lastname: Fang
authors.forename: Ingalill
authors.initials: I
authors.lastname: Avis
authors.forename: David
authors.initials: D
authors.lastname: Salomon
authors.forename: Frank
authors.initials: F
authors.lastname: Cuttitta
keywords: Angiogenesis; Drug screening; Fluorescent; High-throughput; Metastasis; Personalized chemotherapy.; Three-dimensional