title: Selection and evaluation of CO2 tolerant indigenous microalga Scenedesmus dimorphus for unsaturated fatty acid rich lipid production under different culture conditions.
abstract: Five indigenous microalgal strains of Scenedesmus, Chlorococcum, Coelastrum, and Ankistrodesmus genera, isolated from Indian fresh water habitats, were studied for carbon-dioxide tolerance and its effect on growth, lipid and fatty acid profile. Scenedesmus dimorphus strain showed maximum growth (1.5 g/L) and lipid content (17.83% w/w) under CO2 supplementation, hence selected for detailed evaluation. The selected strain was alkaline adapted but tolerated (i) wide range of pH (5-11); (ii) elevated salinity levels (up to 100 mM, NaCl) with low biomass yields and increased carotenoids (19.34 mg/g biomass); (iii) elevated CO2 levels up to 15% v/v with enhancement in specific growth rate (0.137 d(-1)), biomass yield (1.57 g/L), lipid content (19.6% w/w) and CO2 biofixation rate (0.174 g L(-1) d(-1)). Unsaturated fatty acid content (alpha linolenic acid) increased with CO2 supplementation in the strain.
authors.affiliation: Plant Cell Biotechnology (PCBT) Department, CSIR-Central Food Technological Research Institute, Mysore, India.
authors.forename: S
authors.initials: S
authors.lastname: Vidyashankar
authors.forename: K
authors.initials: K
authors.lastname: Deviprasad
authors.forename: V S
authors.initials: VS
authors.lastname: Chauhan
authors.forename: G A
authors.initials: GA
authors.lastname: Ravishankar
authors.forename: R
authors.initials: R
authors.lastname: Sarada
mesh_terms: D000222:Adaptation, Physiological; D000332:Aerobiosis / Q000187:drug effects; D018533:Biomass; D002245:Carbon Dioxide / Q000494:pharmacology*; D002338:Carotenoids / Q000378:metabolism; D018929:Cell Culture Techniques / Q000379:methods*; D002734:Chlorophyll / Q000378:metabolism; D005231:Fatty Acids, Unsaturated / Q000096:biosynthesis*; D006863:Hydrogen-Ion Concentration / Q000187:drug effects; D058086:Microalgae / Q000166:cytology / Q000187:drug effects / Q000254:growth & development* / Q000302:isolation & purification; D009584:Nitrogen / Q000494:pharmacology; D058433:Photobioreactors; D020959:Polyethylene / Q000737:chemistry; D054712:Salinity; D044444:Scenedesmus / Q000166:cytology / Q000187:drug effects / Q000254:growth & development* / Q000302:isolation & purification; D012965:Sodium Chloride / Q000494:pharmacology
chemical_list: D005231:Fatty Acids, Unsaturated; D002734:Chlorophyll; D002245:Carbon Dioxide; D002338:Carotenoids; D012965:Sodium Chloride; D020959:Polyethylene; D009584:Nitrogen
keywords: Alpha linolenic acid; CO(2) biofixation; Scenedesmus dimorphus