title: Real-time detection of DNA cleavage induced by [M(2,2'-bipyridine)2(NO3)](NO3) (M=Cu(II), Zn(II) and Cd(II)) complexes using linear dichroism technique.
abstract: The catalytic effect of [M(2,2'-bipyridine)2(NO3)](NO3) (M(bpy)2, M=Cu(II), Zn(II) and Cd(II)) on the super-coiled and double stranded DNA (scDNA and dsDNA) was examined by electrophoresis and a real-time detection linear dichroism (LD) technique. Although the Cu(bpy)2 complex effectively cleaved both types of DNA, the other two complexes were inactive. This was explained by the electrochemical properties of the metal complexes. The Cu(bpy)2 complex exhibited a redox potential at -0.222V with a peak to peak separation of 0.201V, whereas the other two metal complexes did not undergo any redox reaction. Both electrophoresis and LD measurements revealed the superoxide radical, ·O2(-), to be responsible for DNA cleavage. A kinetic study using the LD technique showed that the cleavage of dsDNA consisted of two first order reactions. The fast reaction is believed to reflect the cleavage of one strand, whereas the slow reaction involves the cleavage of the complementary strand at or near the first cleaved site.
authors.affiliation: Department of Chemistry, Yeungnam University, Gyeongsan City, Gyeong-buk 712-749, Republic of Korea.
authors.forename: Hee-Jin
authors.initials: HJ
authors.lastname: Park
authors.forename: Ji Hye
authors.initials: JH
authors.lastname: Kwon
authors.forename: Tae-Sub
authors.initials: TS
authors.lastname: Cho
authors.forename: Jong Moon
authors.initials: JM
authors.lastname: Kim
authors.forename: In Hong
authors.initials: IH
authors.lastname: Hwang
authors.forename: Cheal
authors.initials: C
authors.lastname: Kim
authors.forename: Soojin
authors.initials: S
authors.lastname: Kim
authors.forename: Jinheung
authors.initials: J
authors.lastname: Kim
authors.forename: Seog K
authors.initials: SK
authors.lastname: Kim
mesh_terms: D015082:2,2'-Dipyridyl / Q000737:chemistry*; D002104:Cadmium / Q000737:chemistry*; D056831:Coordination Complexes / Q000737:chemistry*; D003300:Copper / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D053837:DNA Cleavage; D004563:Electrochemistry; D015394:Molecular Structure; D015032:Zinc / Q000737:chemistry*
chemical_list: D056831:Coordination Complexes; D002104:Cadmium; D015082:2,2'-Dipyridyl; D003300:Copper; D004247:DNA; D015032:Zinc
keywords: DNA cleavage; Linear dichroism; M(2,2′-bipyridine)(2) complex; Oxidation