title: [Effect of the insulation layer of tissue-engineering scaffold on collagen type II expression in the neo-cartilage].
abstract: OBJECTIVE
  To investigate the effect of the insulation layer in the tissue-engineering composite osteochondral scaffolds on collagen type II expression in the neo-cartilage.
  
  
  METHODS
  Chondral phases of insulation layer-free or insulation layer-containing biphasic scaffolds were seeded with autogeneic chondrocyte-induced bone marrow-derived mesenchymal stem cells. Then, the biphasic scaffolds-cells constructs were implanted into osteochondral defects of rabbits' knees. The expression of type II collagen in the tissue-engineering cartilages was evaluated by immunohistochemistry and Western blotting at 3 and 6 months after surgery, respectively. Moreover, the mRNA expression of collagen type II was also detected by qRT-PCR.
  
  
  RESULTS
  The expression of the collagen type II at both protein and mRNA levels in tissue-engineering neo-cartilages generated by the insulation layer-containing biphasic scaffold were significantly higher than those by the insulation layer-free biphasic scaffold in vivo (P<0.05).
  
  
  CONCLUSIONS
  After the insulation layer is added into the osteochondral composite scaffold, the collagen type II expression in the tissue-engineering neo-cartilage can be significantly enhanced.
authors.affiliation: 82nd Hospital of PLA, Huaian 223001, China.
authors.forename: Hu
authors.initials: H
authors.lastname: DA
authors.forename: Yunjing
authors.initials: Y
authors.lastname: Mu
authors.forename: Wenyong
authors.initials: W
authors.lastname: Wang
mesh_terms: D000818:Animals; D001861:Bone Regeneration / Q000235:genetics; D002356:Cartilage / Q000378:metabolism; D020219:Chondrogenesis / Q000235:genetics; D024043:Collagen Type II / Q000235:genetics / Q000378:metabolism*; D005260:Female; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011817:Rabbits; D023822:Tissue Engineering; D054457:Tissue Scaffolds
chemical_list: D024043:Collagen Type II; D012333:RNA, Messenger