title: [Preparation, characterization and Calu-3 cellular uptake of three kinds of poly(b-benzyl-L-amino)block-poly(ethylene glycol) nanoparticles].
abstract: The aim of this paper is to compare the cytotoxicity and cellular uptake efficiency of three kinds of poly(b-benzyl-L-amino) block-poly(ethylene glycol) nanoparticles (PXA-PEG-NPs) using Calu-3 cells, and select one as a nasal drug delivery vector for curcumin (Cur). Poly(gamma-benzyl-L-glutamate) block-poly(ethylene glycol) nanoparticles (PBLG-PEG-NPs), poly(gamma-benzyl-L-lysine) block-poly(ethyleneglycol) nanoparticles (PZLL-PEG-NPs) and poly(gamma-benzyl-L-aspartate) block-poly(ethylene glycol) nanoparticles (PBLA-PEG-NPs) were prepared by emulsion-solvent evaporation method. MTT assays were used to evaluate the cytotoxicity of PXA-PEG-NPs against Calu-3 cells. The cellular uptake of nanoparticles was visualized by an inverted fluorescence microscope and quantified by a flow cytometer. The results indicated that even at high concentration of 2 mg x mL(-1) the three nanoparticles had no cytotoxicity on Calu-3 cells. Compared to the curcumin solution, the three curcumin-loaded PXA-PEG-NPs showed significantly higher cellular uptake efficiency on Calu-3 cells (at equal concentration of curcumin with 5 microg x mL(-1) Cur solution), PBLG-PEG-NPs group was the highest. The cellular uptake increased with incubation time, and has positive correlation with nanoparticle concentration. In brief, PXA-PEG-NPs are conducive to delivery Cur into cells, and PBLG-PEG-NPs might be provided as a good nasal drug delivery carrier.
authors.affiliation: School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, China.
authors.forename: Yin
authors.initials: Y
authors.lastname: Zhou
authors.forename: Li-Na
authors.initials: LN
authors.lastname: Lu
authors.forename: Xue
authors.initials: X
authors.lastname: Xin
authors.forename: Dong-Feng
authors.initials: DF
authors.lastname: Huo
authors.forename: Hong-Bing
authors.initials: HB
authors.lastname: Wu
authors.forename: Ming-Feng
authors.initials: MF
authors.lastname: Qiu
mesh_terms: D000230:Adenocarcinoma / Q000378:metabolism / Q000473:pathology; D000281:Administration, Intranasal; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000008:administration & dosage / Q000378:metabolism; D001224:Aspartic Acid / Q000737:chemistry / Q000633:toxicity; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D003474:Curcumin / Q000008:administration & dosage* / Q000378:metabolism*; D004337:Drug Carriers; D019855:Ethylene Glycol / Q000737:chemistry / Q000633:toxicity; D006801:Humans; D008175:Lung Neoplasms / Q000378:metabolism / Q000473:pathology; D008239:Lysine / Q000737:chemistry / Q000633:toxicity; D053758:Nanoparticles; D010316:Particle Size; D011092:Polyethylene Glycols / Q000737:chemistry* / Q000633:toxicity; D011099:Polyglutamic Acid / Q000031:analogs & derivatives* / Q000737:chemistry / Q000633:toxicity
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D004337:Drug Carriers; C003172:poly-gamma-benzyl-L-glutamate; D011099:Polyglutamic Acid; D001224:Aspartic Acid; D011092:Polyethylene Glycols; D019855:Ethylene Glycol; D003474:Curcumin; D008239:Lysine