title: WWOX expression in giant cell lesions of the jaws.
abstract: OBJECTIVE
  To investigate WWOX messenger RNA (mRNA) transcriptional levels in giant cell lesions (GCLs) of the jaws and associate its expression with clinical parameters.
  
  
  METHODS
  In this pilot study, quantitative reverse-transcription polymerase chain reaction was performed to analyze WWOX expression in 6 central giant cell lesions (CGCLs) (including 2 aggressive), 5 peripheral giant cell lesions (PGCLs) and 1 cherubism sample. Immunohistochemistry was performed to confirm the localization of the Wwox protein.
  
  
  RESULTS
  CGCL and PGCL showed an overall increased expression of WWOX, as did the cherubism case, but no differences were observed among the groups. Wwox was localized almost entirely to the cytoplasm of multinucleated giant cells, as well as in a few mononuclear cells. CGCL and PGCL showed higher expression of the WWOX mRNA than peripheral blood mononuclear cells. The 2 aggressive CGCL samples exhibited decreased WWOX expression.
  
  
  CONCLUSIONS
  These results showed increased expression of WWOX mainly in non-aggressive GCLs of the jaws.
authors.affiliation: Department of Oral Surgery and Pathology, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
authors.forename: Fabrício Rezende
authors.initials: FR
authors.lastname: Amaral
authors.forename: Marina Gonçalves
authors.initials: MG
authors.lastname: Diniz
authors.forename: Vanessa Fátima
authors.initials: VF
authors.lastname: Bernardes
authors.forename: Paulo Eduardo Alencar
authors.initials: PE
authors.lastname: Souza
authors.forename: Ricardo Santiago
authors.initials: RS
authors.lastname: Gomez
authors.forename: Carolina Cavaliéri
authors.initials: CC
authors.lastname: Gomes
mesh_terms: D000328:Adult; D017209:Apoptosis / Q000502:physiology*; D002636:Cherubism / Q000473:pathology; D002648:Child; D003593:Cytoplasm / Q000473:pathology; D005260:Female; D015726:Giant Cells / Q000473:pathology; D005882:Gingival Diseases / Q000473:pathology; D006101:Granuloma, Giant Cell / Q000473:pathology*; D006801:Humans; D007150:Immunohistochemistry; D007571:Jaw Diseases / Q000473:pathology*; D007963:Leukocytes, Mononuclear / Q000473:pathology; D008297:Male; D008336:Mandibular Diseases / Q000473:pathology; D008439:Maxillary Diseases / Q000473:pathology; D008875:Middle Aged; D010088:Oxidoreductases / Q000032:analysis*; D010865:Pilot Projects; D020133:Reverse Transcriptase Polymerase Chain Reaction; D025521:Tumor Suppressor Proteins / Q000032:analysis*; D000074584:WW Domain-Containing Oxidoreductase; D055815:Young Adult
chemical_list: D025521:Tumor Suppressor Proteins; D010088:Oxidoreductases; D000074584:WW Domain-Containing Oxidoreductase; C486071:WWOX protein, human