title: Comparison of different extraction methods for the determination of α- and β-thujone in sage (Salvia officinalis L.) herbal tea.
abstract: Salvia officinalis L. (sage) is an important industrial plant used both for food and pharmaceutical purposes. The terpene fraction of this plant is responsible for many of its therapeutic and culinary properties. We used different extraction methods Tenax TA® purge and trap, headspace (HS) solid-phase microextraction, HS sorptive extraction, and stir bar sorptive extraction to analyze the terpene fraction extracted from sage tea by GC-MS. Twenty compounds were identified, including α-, β-thujone, and several other oxygenated monoterpenes (1,8-cineole, linalool, camphor, boneol, and bornyl acetate) and oxygenated sesquiterpenes (caryophyllene oxide, viridiflorol, humulene epoxide I, II, and III). Tenax TA® and HS sorptive extraction extracted a lower number of identified compounds, whereas HS solid-phase microextraction allowed the complete extraction of volatiles with particular reference to α- and β-thujone. The importance of the determination of thujones content in sage herbal tea is also discussed.
authors.affiliation: Department of Analytical Chemistry, Medical University of Gdansk, Gdansk, Poland.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Arceusz
authors.forename: Andrea
authors.initials: A
authors.lastname: Occhipinti
authors.forename: Andrea
authors.initials: A
authors.lastname: Capuzzo
authors.forename: Massimo E
authors.initials: ME
authors.lastname: Maffei
mesh_terms: D001628:Beverages / Q000032:analysis*; D000081008:Bicyclic Monoterpenes; D039821:Monoterpenes / Q000302:isolation & purification*; D018515:Plant Leaves / Q000737:chemistry; D027543:Salvia officinalis / Q000737:chemistry*
chemical_list: D000081008:Bicyclic Monoterpenes; D039821:Monoterpenes; C005790:beta-thujone
keywords: Headspace analysis; Oxygenated monoterpenes; Sage tea; Salvia officinalis; Thujone