title: A novel aspartic acid protease gene from pineapple fruit (Ananas comosus): cloning, characterization and relation to postharvest chilling stress resistance.
abstract: A full-length cDNA encoding a putative aspartic acid protease (AcAP1) was isolated for the first time from the flesh of pineapple (Ananas comosus) fruit. The deduced sequence of AcAP1 showed all the common features of a typical plant aspartic protease phytepsin precursor. Analysis of AcAP1 gene expression under postharvest chilling treatment in two pineapple varieties differing in their resistance to blackheart development revealed opposite trends. The resistant variety showed an up-regulation of AcAP1 precursor gene expression whereas the susceptible showed a down-regulation in response to postharvest chilling treatment. The same trend was observed regarding specific AP enzyme activity in both varieties. Taken together our results support the involvement of AcAP1 in postharvest chilling stress resistance in pineapple fruits.
authors.affiliation: IBIOS-UMR CNRS 7618 BIOEMCO, Université Paris Est Créteil (UPEC), 61 Avenue du Général de Gaulle, 94010 Créteil Cedex 2, France; CIRAD-PRAM, Quartier Petite Morne, BP214, 97285 Le Lamentin, Martinique, France.
authors.forename: Astrid-Kim
authors.initials: AK
authors.lastname: Raimbault
authors.forename: Yasmine
authors.initials: Y
authors.lastname: Zuily-Fodil
authors.forename: Alain
authors.initials: A
authors.lastname: Soler
authors.forename: Maria H
authors.initials: MH
authors.lastname: Cruz de Carvalho
mesh_terms: D031230:Ananas / Q000235:genetics* / Q000378:metabolism; D057055:Aspartic Acid Proteases / Q000737:chemistry / Q000235:genetics*; D003001:Cloning, Molecular; D003080:Cold Temperature; D018076:DNA, Complementary / Q000737:chemistry; D010802:Phylogeny; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics*; D020539:Sequence Analysis, Protein; D013312:Stress, Physiological / Q000235:genetics
chemical_list: D018076:DNA, Complementary; D010940:Plant Proteins; D057055:Aspartic Acid Proteases
keywords: AP; Aspartic acid protease; Blackheart; EDTA; PCT; PSI; Pineapple; Postharvest chilling treatment; RACE; RT-qPCR; Stress resistance; aspartic acid protease; ethylenediamine-tetraacetic acid; plant specific insert; postharvest chilling treatment; rapid amplification of cDNA ends; reverse transcription-quantitative polymerase chain reaction