title: Use of RNAlater as a preservation method for parasitic coprology studies in wild-living chimpanzees.
abstract: We evaluated the use of an RNA stabilisation buffer, RNAlater (Ambion, Austin, Texas), as a preservation medium for parasitic coprology analysis of faecal samples collected from chimpanzees living in the wild (Pan troglodytes troglodytes). Thirty faecal samples collected in the forests of south-east Cameroon (Mambele area) from 2003 to 2011 were preserved in RNAlater at -80 °C and analysed for their parasite content. We identified and counted parasitic elements and assessed their shape, size and morphology in relation to the storage time of the samples. We found that parasite elements were identifiable in RNAlater preserved samples after as many as 7 years, showing that RNAlater could be an effective and reliable preservation medium for coprology. Thus, its use could be an interesting way to optimise sample collection for several types of studies (parasitology and bacteriology/virology) at once, especially considering the logistically challenging and time-consuming field campaigns needed to obtain these faecal samples.
authors.affiliation: UMI 233 "TransVIHMI", Institut de Recherche pour le Developpement (IRD), University of Montpellier 1 (UM1), Montpellier, France. Electronic address: pascal.drakulovski@univ-montp1.fr.
authors.forename: P
authors.initials: P
authors.lastname: Drakulovski
authors.forename: S
authors.initials: S
authors.lastname: Locatelli
authors.forename: C
authors.initials: C
authors.lastname: Butel
authors.forename: S
authors.initials: S
authors.lastname: Pion
authors.forename: D
authors.initials: D
authors.lastname: Krasteva
authors.forename: E
authors.initials: E
authors.lastname: Mougdi-Pole
authors.forename: E
authors.initials: E
authors.lastname: Delaporte
authors.forename: M
authors.initials: M
authors.lastname: Peeters
authors.forename: M
authors.initials: M
authors.lastname: Mallié
mesh_terms: D000818:Animals; D000835:Animals, Wild; D018420:Ape Diseases / Q000469:parasitology*; D002021:Buffers; D005243:Feces / Q000469:parasitology*; D002679:Pan troglodytes / Q000469:parasitology*; D010271:Parasites / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D010273:Parasitic Diseases, Animal / Q000469:parasitology*; D011309:Preservation, Biological / Q000379:methods*; D012313:RNA / Q000592:standards
chemical_list: D002021:Buffers; D012313:RNA
keywords: Endoparasites; Parasitic coprology; RNAlater®; Wild-living chimpanzees