title: FGF-2 angiogenesis in bone regeneration within critical-sized bone defects in rat calvaria.
abstract: OBJECTIVE
  This study examined the effect of basic fibroblast growth factor (FGF)-2 with an absorbable collagen sponge on angiogenesis and bone regeneration in rat calvarial critical-sized bone defects using microcomputed tomography.
  
  
  METHODS
  Forty 7-week-old male Fischer rats were used. The symmetrical critical-sized calvarial bone defects (5 mm diameter) were created. An absorbable collagen sponge with or without FGF-2 (0.1% or 0.3%) was implanted into each bone defect.
  
  
  RESULTS
  Blood vessel and bone volumes were significantly higher in the 0.3% FGF-2 group compared with the control and 0.1% FGF-2 groups on day 28. Significantly more osteoblast- and osteoclast-like cells were seen in the 0.3% FGF-2 group.
  
  
  CONCLUSIONS
  Thus, FGF-2 increased blood vessel and bone formation in rat calvarial critical-sized bone defects.
authors.affiliation: Division of Applied Oral Sciences, Nihon University Graduate School of Dentistry, Tokyo, Japan.
authors.forename: Risa
authors.initials: R
authors.lastname: Kigami
authors.forename: Shuichi
authors.initials: S
authors.lastname: Sato
authors.forename: Noriko
authors.initials: N
authors.lastname: Tsuchiya
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Yoshimakai
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Arai
authors.forename: Koichi
authors.initials: K
authors.lastname: Ito
mesh_terms: D020341:Absorbable Implants; D000818:Animals; D001847:Bone Diseases / Q000473:pathology / Q000601:surgery*; D001861:Bone Regeneration / Q000187:drug effects*; D003094:Collagen; D003287:Contrast Media; D016222:Fibroblast Growth Factor 2 / Q000627:therapeutic use*; D007091:Image Processing, Computer-Assisted / Q000379:methods; D007479:Iopamidol; D008297:Male; D055806:Microvessels / Q000187:drug effects / Q000473:pathology; D018919:Neovascularization, Physiologic / Q000187:drug effects*; D010006:Osteoblasts / Q000187:drug effects / Q000473:pathology; D010010:Osteoclasts / Q000187:drug effects / Q000473:pathology; D010012:Osteogenesis / Q000187:drug effects; D010294:Parietal Bone / Q000098:blood supply / Q000601:surgery*; D051381:Rats; D011916:Rats, Inbred F344; D013997:Time Factors; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds; D014963:X-Ray Intensifying Screens; D055114:X-Ray Microtomography / Q000379:methods
chemical_list: D003287:Contrast Media; D016222:Fibroblast Growth Factor 2; D003094:Collagen; D007479:Iopamidol