title: Structural and functional evolution of positively selected sites in pine glutathione S-transferase enzyme family.
abstract: Phylogenetic analyses have identified positive selection as an important driver of protein evolution, both structural and functional. However, the lack of appropriate combined functional and structural assays has generally hindered attempts to elucidate patterns of positively selected sites and their effects on enzyme activity and substrate specificity. In this study we investigated the evolutionary divergence of the glutathione S-transferase (GST) family in Pinus tabuliformis, a pine that is widely distributed from northern to central China, including cold temperate and drought-stressed regions. GSTs play important roles in plant stress tolerance and detoxification. We cloned 44 GST genes from P. tabuliformis and found that 26 of the 44 belong to the largest (Tau) class of GSTs and are differentially expressed across tissues and developmental stages. Substitution models identified five positively selected sites in the Tau GSTs. To examine the functional significance of these positively selected sites, we applied protein structural modeling and site-directed mutagenesis. We found that four of the five positively selected sites significantly affect the enzyme activity and specificity; thus their variation broadens the GST family substrate spectrum. In addition, positive selection has mainly acted on secondary substrate binding sites or sites close to (but not directly at) the primary substrate binding site; thus their variation enables the acquisition of new catalytic functions without compromising the protein primary biochemical properties. Our study sheds light on selective aspects of the functional and structural divergence of the GST family in pine and other organisms.
authors.affiliation: State Key Laboratory of Systematic and Evolutionary Botany, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Lan
authors.forename: Xiao-Ru
authors.initials: XR
authors.lastname: Wang
authors.forename: Qing-Yin
authors.initials: QY
authors.lastname: Zeng
mesh_terms: D001665:Binding Sites; D019143:Evolution, Molecular; D005982:Glutathione Transferase / Q000737:chemistry / Q000235:genetics; D008958:Models, Molecular; D028223:Pinus / Q000201:enzymology / Q000235:genetics; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics
chemical_list: D010940:Plant Proteins; D005982:Glutathione Transferase
keywords: Enzyme Mutation; Molecular Evolution; Plant Biochemistry; Protein Evolution; Protein Structure