title: Genomic adaptation of the ISA virus to Salmo salar codon usage.
abstract: BACKGROUND
  The ISA virus (ISAV) is an Orthomyxovirus whose genome encodes for at least 10 proteins. Low protein identity and lack of genetic tools have hampered the study of the molecular mechanism behind its virulence. It has been shown that viral codon usage controls several processes such as translational efficiency, folding, tuning of protein expression, antigenicity and virulence. Despite this, the possible role that adaptation to host codon usage plays in virulence and viral evolution has not been studied in ISAV.
  
  
  METHODS
  Intergenomic adaptation between viral and host genomes was calculated using the codon adaptation index score with EMBOSS software and the Kazusa database. Classification of host genes according to GeneOnthology was performed using Blast2go. A non parametric test was applied to determine the presence of significant correlations among CAI, mortality and time.
  
  
  RESULTS
  Using the codon adaptation index (CAI) score, we found that the encoding genes for nucleoprotein, matrix protein M1 and antagonist of Interferon I signaling (NS1) are the ISAV genes that are more adapted to host codon usage, in agreement with their requirement for production of viral particles and inactivation of antiviral responses. Comparison to host genes showed that ISAV shares CAI values with less than 0.45% of Salmo salar genes. GeneOntology classification of host genes showed that ISAV genes share CAI values with genes from less than 3% of the host biological process, far from the 14% shown by Influenza A viruses and closer to the 5% shown by Influenza B and C. As well, we identified a positive correlation (p<0.05) between CAI values of a virus and the duration of the outbreak disease in given salmon farms, as well as a weak relationship between codon adaptation values of PB1 and the mortality rates of a set of ISA viruses.
  
  
  CONCLUSIONS
  Our analysis shows that ISAV is the least adapted viral Salmo salar pathogen and Orthomyxovirus family member less adapted to host codon usage, avoiding the general behavior of host genes. This is probably due to its recent emergence among farmed Salmon populations.
authors.affiliation: Centro de Biotecnología Acuícola, Laboratorio de Virología, Facultad de Química y Biología, Universidad de Santiago de Chile, Avenida Libertador Bernardo O'Higgins 3363, Santiago, Chile. mario.tello@usach.cl
authors.forename: Mario
authors.initials: M
authors.lastname: Tello
authors.forename: Francisco
authors.initials: F
authors.lastname: Vergara
authors.forename: Eugenio
authors.initials: E
authors.lastname: Spencer
mesh_terms: D000220:Adaptation, Biological; D000818:Animals; D003062:Codon; D019295:Computational Biology; D004196:Disease Outbreaks; D005393:Fish Diseases / Q000453:epidemiology / Q000473:pathology / Q000821:virology*; D046829:Isavirus / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity; D009976:Orthomyxoviridae Infections / Q000453:epidemiology / Q000473:pathology / Q000662:veterinary* / Q000821:virology; D020317:Salmo salar / Q000235:genetics*; D016019:Survival Analysis; D013997:Time Factors
chemical_list: D003062:Codon