title: A microarray method for identifying tumor antigens by screening a tumor cDNA expression library against cancer sera.
abstract: The immune system responds to tumor cells. The challenge has been how to effectively use these responses to treat or protect against cancer. Toward the goal of developing a cancer vaccine, we are pursuing methodologies for the discovery and testing of useful antigens. We present an array-based approach for discovering these B cell antigens by directly screening for specific host-sera reactivity to lysates from tumor-derived cDNA expression libraries. Several cancer-specific antigens were identified, and these are currently being validated as potential candidates.
authors.affiliation: Center for Innovations in Medicine; The Biodesign Institute; Arizona State University; Tempe, AZ USA; Biological Design Program; Arizona State University; Tempe, AZ USA.
authors.forename: Kurt
authors.initials: K
authors.lastname: Whittemore
authors.affiliation: Center for Innovations in Medicine; The Biodesign Institute; Arizona State University; Tempe, AZ USA; HealthTell, Inc.; Chandler, AZ USA.
authors.forename: Kathryn
authors.initials: K
authors.lastname: Sykes
mesh_terms: D000818:Animals; D000951:Antigens, Neoplasm / Q000032:analysis* / Q000235:genetics; D055808:Drug Discovery / Q000379:methods*; D005260:Female; D015723:Gene Library; D006801:Humans; D008807:Mice, Inbred BALB C; D046228:Microarray Analysis / Q000379:methods*
chemical_list: D000951:Antigens, Neoplasm
keywords: antibody; cDNA; cancer; immunogen; library; sera; tumor; vaccine