title: Biocompatibility and favorable response of mesenchymal stem cells on fibronectin-gold nanocomposites.
abstract: A simple surface modification method, comprising of a thin coating with gold nanoparticles (AuNPs) and fibronectin (FN), was developed to improve the biocompatibility required for cardiovascular devices. The nanocomposites from FN and AuNPs (FN-Au) were characterized by the atomic force microscopy (AFM), UV-Vis spectrophotometry (UV-Vis), and Fourier transform infrared spectroscopy (FTIR). The biocompatibility of the nanocomposites was evaluated by the response of monocytes and platelets to the material surface in vitro. FN-Au coated surfaces demonstrated low monocyte activation and platelet activation. The behavior of human umbilical cord-derived mesenchymal stem cells (MSCs) on FN-Au was further investigated. MSCs on FN-Au nanocomposites particularly that containing 43.5 ppm of AuNPs (FN-Au 43.5 ppm) showed cell proliferation, low ROS generation, as well as increases in the protein expression levels of matrix metalloproteinase-9 (MMP-9) and endothelial nitric oxide synthase (eNOS), which may account for the enhanced MSC migration on the nanocomposites. These results suggest that the FN-Au nanocomposite thin film coating may serve as a potential and simple solution for the surface modification of blood-contacting devices such as vascular grafts.
authors.affiliation: Graduate Institute of Basic Medical Science, China Medical University, Taichung, Taiwan, ROC.
authors.forename: Huey-Shan
authors.initials: HS
authors.lastname: Hung
authors.forename: Cheng-Ming
authors.initials: CM
authors.lastname: Tang
authors.forename: Chien-Hsun
authors.initials: CH
authors.lastname: Lin
authors.forename: Shinn-Zong
authors.initials: SZ
authors.lastname: Lin
authors.forename: Mei-Yun
authors.initials: MY
authors.lastname: Chu
authors.forename: Wei-Shen
authors.initials: WS
authors.lastname: Sun
authors.forename: Wei-Chien
authors.initials: WC
authors.lastname: Kao
authors.forename: Hsieh
authors.initials: H
authors.lastname: Hsien-Hsu
authors.forename: Chih-Yang
authors.initials: CY
authors.lastname: Huang
authors.forename: Shan-hui
authors.initials: SH
authors.lastname: Hsu
mesh_terms: D001672:Biocompatible Materials; D049109:Cell Proliferation; D005353:Fibronectins / Q000737:chemistry*; D005434:Flow Cytometry; D006046:Gold / Q000737:chemistry*; D006801:Humans; D020782:Matrix Metalloproteinases / Q000378:metabolism; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000201:enzymology / Q000378:metabolism; D053761:Nanocomposites / Q000737:chemistry*; D017382:Reactive Oxygen Species / Q000378:metabolism; D013055:Spectrophotometry, Infrared; D013056:Spectrophotometry, Ultraviolet
chemical_list: D001672:Biocompatible Materials; D005353:Fibronectins; D017382:Reactive Oxygen Species; D006046:Gold; D020782:Matrix Metalloproteinases