title: Expression patterns of Th1/Th2 transcription factors in patients with guttate psoriasis.
abstract: Many lines of evidence propose that psoriasis is a T cell-mediated disease where T cell activation is followed by secretion of inflammatory cytokines. To elucidate the functional state of T cells in guttate psoriasis, we analysed mRNA expression levels of T-bet and GATA-3 for Th1 and Th2 differentiation, respectively together with Th1 (IFN-γ) and Th2 (IL-4) cytokine mRNA expression. Relative quantification of T-bet, GATA-3, IFN-γ and Th2, and IL-4 transcripts in peripheral blood leukocytes (PBL) was conducted by real-time reverse transcriptase PCR (RT-PCR). Serum levels of IFN-γ and Th2 and IL-4 were also determined by ELISA. GATA-3 and IL-4 mRNA expression levels were lower in psoriatic patients as compared to normal healthy controls. The expression levels of T-bet and IFN-γ and Th2 genes were relatively similar in the patients and controls. In addition, a marked decrease in plasma IL-4 levels was observed in the psoriasis group, while no differences were observed with regard to levels of IFN-γ and Th2 between patients and normal subjects. Furthermore, a clear correlation between decreased IL-4 mRNA expression and IL-4 (P < 0.05) was revealed. These results suggested that altered balance between Th1 and Th2 cells transcription factor genes and they products may be implicated in the pathogenesis of psoriasis.
authors.affiliation: National Institute of Genetic Engineering and Biotechnology, Clinical Genetics Department, Tehran, Iran.
authors.forename: Elaheh
authors.initials: E
authors.lastname: Vanaki
authors.forename: Mitra
authors.initials: M
authors.lastname: Ataei
authors.forename: Mohammad Hossein
authors.initials: MH
authors.lastname: Sanati
authors.forename: Parvine
authors.initials: P
authors.lastname: Mansouri
authors.forename: Mahmoud
authors.initials: M
authors.lastname: Mahmoudi
authors.forename: Feresteh
authors.initials: F
authors.lastname: Zarei
authors.forename: Zohreh
authors.initials: Z
authors.lastname: Jadali
mesh_terms: D000328:Adult; D005260:Female; D050990:GATA3 Transcription Factor / Q000235:genetics / Q000378:metabolism; D006801:Humans; D007371:Interferon-gamma / Q000235:genetics / Q000378:metabolism; D015847:Interleukin-4 / Q000235:genetics; D008297:Male; D008875:Middle Aged; D011565:Psoriasis / Q000235:genetics* / Q000378:metabolism; D020825:T-Box Domain Proteins / Q000235:genetics / Q000378:metabolism; D018417:Th1 Cells / Q000378:metabolism*; D018418:Th2 Cells / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism; D055815:Young Adult
chemical_list: D050990:GATA3 Transcription Factor; D020825:T-Box Domain Proteins; D014157:Transcription Factors; D015847:Interleukin-4; D007371:Interferon-gamma