title: Let-7c inhibits A549 cell proliferation through oncogenic TRIB2 related factors.
abstract: MicroRNAs have tumor suppressive or oncogenic roles in carcinogenesis. This study aimed to investigate the mechanism of let-7c in suppressing lung cancer cell proliferation. First, let-7c was revealed to be able to inhibit lung adenocarcinoma cell proliferation significantly. TRIB2 was further demonstrated to be a novel target and negatively regulated by let-7c. As downstream signals of TRIB2, the activities of C/EBP-α and phosphorylated p38MAPK were increased obviously in let-7c-treated cells compared with controls. Our results demonstrate that, through regulating the expression of TRIB2 and its downstream factors, let-7c can effectively inhibit A549 cell proliferation in vitro and in vivo.
authors.affiliation: Key Laboratory of Tumor Molecular Biology in Binzhou Medical University, Department of Biochemistry and Molecular Biology, Binzhou Medical University, YanTai, ShanDong 264003, PR China.
authors.forename: Ping-Yu
authors.initials: PY
authors.lastname: Wang
authors.forename: Yun-Xiao
authors.initials: YX
authors.lastname: Sun
authors.forename: Shuai
authors.initials: S
authors.lastname: Zhang
authors.forename: Min
authors.initials: M
authors.lastname: Pang
authors.forename: Han-Han
authors.initials: HH
authors.lastname: Zhang
authors.forename: Shu-Yan
authors.initials: SY
authors.lastname: Gao
authors.forename: Can
authors.initials: C
authors.lastname: Zhang
authors.forename: Chang-Jun
authors.initials: CJ
authors.lastname: Lv
authors.forename: Shu-Yang
authors.initials: SY
authors.lastname: Xie
mesh_terms: D000818:Animals; D017209:Apoptosis; D022763:CCAAT-Enhancer-Binding Protein-alpha / Q000378:metabolism; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D002453:Cell Cycle; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015972:Gene Expression Regulation, Neoplastic; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000378:metabolism*; D008175:Lung Neoplasms / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008819:Mice, Nude; D035683:MicroRNAs / Q000378:metabolism*; D009368:Neoplasm Transplantation; D016376:Oligonucleotides, Antisense / Q000378:metabolism; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism
chemical_list: D022763:CCAAT-Enhancer-Binding Protein-alpha; D047908:Intracellular Signaling Peptides and Proteins; D035683:MicroRNAs; D016376:Oligonucleotides, Antisense; C487931:mirnlet7 microRNA, human; D049452:Green Fluorescent Proteins; D017871:Calcium-Calmodulin-Dependent Protein Kinases; C489044:TRIB2 protein, human; D048051:p38 Mitogen-Activated Protein Kinases
keywords: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; ASO; C/EBP; CCAAT/enhancer-binding protein; Cell proliferation; Gene expression; HCC; Let-7c; Lung adenocarcinoma; MAPK; MTT; TRIB2 gene; antisense oligonucleotides; hepatocellular carcinoma; miRNAs; microRNAs; mitogen-activated protein kinase