title: Cytoplasmic HuR expression: correlation with cellular inhibitors of apoptosis protein-2 expression and clinicopathologic factors in oral squamous cell carcinoma cells.
abstract: BACKGROUND
  HuR expression has been noted in several cancer types, in which it may contribute to increased expression of cellular inhibitors of apoptosis protein-2 (cIAP2) observed during tumorigenesis.
  
  
  METHODS
  To assess the correlation between cIAP2 and HuR in cases of oral squamous cell carcinoma (OSCC), the expression patterns of HuR and cIAP2 were assessed by immunohistochemical analyses of 95 treated OSCC samples.
  
  
  RESULTS
  In the tumor tissues, positive cytoplasmic HuR expression was evident in 71.6% of samples and positive cIAP2 expression was noted in 95.8% of samples. Positive cytoplasmic HuR expression was significantly associated with positive cIAP2 (p < .035) and high cIAP2 expression (p < .007), as well as high grade (p < .050). The inhibition of HuR expression by small interfering RNA or leptomycin B caused a reduction in the inducibility of cIAP2 in oral cancer cells.
  
  
  CONCLUSIONS
  Cytoplasmic expression of HuR is associated with cIAP2 expression in OSCCs.
authors.affiliation: Department of Research Development, Institute of Jinan Red Ginseng, Jinan-gun, South Korea.
authors.forename: Jeong-Dan
authors.initials: JD
authors.lastname: Cha
authors.forename: Hee-Kyeong
authors.initials: HK
authors.lastname: Kim
authors.forename: In-Ho
authors.initials: IH
authors.lastname: Cha
mesh_terms: D000368:Aged; D000075747:Baculoviral IAP Repeat-Containing 3 Protein; D015153:Blotting, Western; D002294:Carcinoma, Squamous Cell / Q000378:metabolism* / Q000401:mortality; D018929:Cell Culture Techniques; D045744:Cell Line, Tumor; D003593:Cytoplasm / Q000378:metabolism; D051959:ELAV Proteins / Q000378:metabolism*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D051034:Inhibitor of Apoptosis Proteins / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D056910:Republic of Korea; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015996:Survival Rate; D014162:Transfection; D044767:Ubiquitin-Protein Ligases
chemical_list: D051959:ELAV Proteins; D051034:Inhibitor of Apoptosis Proteins; C494832:BIRC3 protein, human; D000075747:Baculoviral IAP Repeat-Containing 3 Protein; D044767:Ubiquitin-Protein Ligases
keywords: HuR; cellular inhibitors of apoptosis protein-2 (cIAP2); inhibitors of apoptosis proteins; mRNA stability; oral squamous cell carcinoma