title: Molecular characterization and phylogenetic analysis of the reticuloendotheliosis virus isolated from wild birds in Northeast China.
abstract: To analyze the status of reticuloendotheliosis (RE) infection of wild birds in China, 585 samples from wild birds collected in Liaoning, Jilin and Heilongjiang provinces China were investigated and analyzed. The sampled birds represent 3 orders and more than 40 species. Virus isolation and PCR amplification showed that some of the wild birds were infected with REV, and 10 REV strains were isolated. The gp90 gene from each of the 10 REV strains was amplified, cloned, and sequenced. Sequence analysis indicated that the gp90 genes of the 10 REV strains isolated in this study were more similar at the nucleotide level with the northeast Chinese strains HLJR0901 and HLJR0801 and some REV strains found in the US and Taiwan than with the early Chinese REV isolate HA9901. Furthermore, phylogenetic analysis indicated that the gp90 genes of the 10 REV strains were more similar to the REV subtype III-representing strain (CSV) than to strains 170A (subtype I) or SNV (subtype II). This is the first study to investigate the status of wild birds infected with REV. The results of this paper will not only provide necessary information for further understanding the evolution of REV, but they also identify the potential role of wild birds in REV transmission and furthers our understanding of the ecology of REV in wild bird species.
authors.affiliation: Institute of Animal Husbandry Veterinary, Liaoning Medical University, Jinzhou 121001, China.
authors.forename: Lili
authors.initials: L
authors.lastname: Jiang
authors.forename: Xiaole
authors.initials: X
authors.lastname: Qi
authors.forename: Yulong
authors.initials: Y
authors.lastname: Gao
authors.forename: Yuping
authors.initials: Y
authors.lastname: Hua
authors.forename: Kai
authors.initials: K
authors.lastname: Li
authors.forename: Xiaoyun
authors.initials: X
authors.lastname: Deng
authors.forename: Qi
authors.initials: Q
authors.lastname: Wang
authors.forename: Lizhou
authors.initials: L
authors.lastname: Zhang
authors.forename: Hongliang
authors.initials: H
authors.lastname: Chai
authors.forename: Yuming
authors.initials: Y
authors.lastname: Chen
authors.forename: Chunhong
authors.initials: C
authors.lastname: Yin
authors.forename: Honglei
authors.initials: H
authors.lastname: Gao
authors.forename: Liting
authors.initials: L
authors.lastname: Qin
authors.forename: Yongqiang
authors.initials: Y
authors.lastname: Wang
authors.forename: Yue
authors.initials: Y
authors.lastname: Qu
authors.forename: Qiang
authors.initials: Q
authors.lastname: Chen
authors.forename: Zhaobin
authors.initials: Z
authors.lastname: Fan
authors.forename: Xiaomei
authors.initials: X
authors.lastname: Wang
mesh_terms: D000818:Animals; D000835:Animals, Wild / Q000145:classification / Q000821:virology*; D001483:Base Sequence; D001715:Bird Diseases / Q000821:virology*; D001717:Birds; D002681:China; D008969:Molecular Sequence Data; D010802:Phylogeny; D001356:Reticuloendotheliosis virus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D012192:Retroviridae Infections / Q000662:veterinary* / Q000821:virology; D014412:Tumor Virus Infections / Q000662:veterinary* / Q000821:virology; D014764:Viral Proteins / Q000235:genetics
chemical_list: D014764:Viral Proteins
keywords: Characterization; Isolation; Reticuloendotheliosis virus; Wild birds