title: The effect of probiotic Escherichia coli strain Nissle 1917 lipopolysaccharide on the 5-aminosalicylic acid transepithelial transport across Caco-2 cell monolayers.
abstract: The object of this study was to investigate the effect of probiotic Escherichia coli strain Nissle 1917 (EcN) (i) EcN lipopolysaccharide (EcN LPS) and (ii) bacteria-free supernatant of EcN suspension (EcN supernatant) on in vitro transepithelial transport of mesalazine (5-aminosalicylic acid, 5-ASA), the most commonly prescribed anti-inflammatory drug in inflammatory bowel disease (IBD). Effect of co-administered EcN LPS (100 µg/ml) or EcN supernatant (50 µg/ml) on the 5-ASA transport (300 µmol/l) was studied using the Caco-2 monolayer (a human colon carcinoma cell line) as a model of human intestinal absorption. Permeability characteristics for absorptive and secretory transport of parent drug and its intracellularly-formed metabolite were determined. The quantification of 5-ASA and its main metabolite N-acetyl-5-amino-salicylic acid (N-Ac-5-ASA) was performed by high performance liquid chromatography. Obtained results suggest that neither EcN LPS nor EcN supernatant had effect on the total 5-ASA transport (secretory flux greater than absorptive flux) and on the transport of intracellularly formed N-Ac-5-ASA (preferentially transported in the secretory direction). The percent cumulative transport of the total 5-ASA alone or in combination with EcN LPS or EcN supernatant did not exceed 1%.
authors.affiliation: Institute of Experimental Biopharmaceutics, Joint Research Centre of PRO.MED.CS Praha a.s. and the Academy of Sciences of the Czech Republic, Hradec Králové, Czech Republic. stetinovaulmanova@seznam.cz.
authors.forename: Věra
authors.initials: V
authors.lastname: Stětinová
authors.forename: Libuše
authors.initials: L
authors.lastname: Smetanová
authors.forename: Dagmar
authors.initials: D
authors.lastname: Kholová
authors.forename: Jaroslav
authors.initials: J
authors.lastname: Květina
authors.forename: Zbyněk
authors.initials: Z
authors.lastname: Svoboda
authors.forename: Zdeněk
authors.initials: Z
authors.lastname: Zídek
authors.forename: Helena
authors.initials: H
authors.lastname: Tlaskalová-Hogenová
mesh_terms: D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000378:metabolism*; D001692:Biological Transport / Q000187:drug effects; D018938:Caco-2 Cells; D017077:Culture Media, Conditioned / Q000737:chemistry; D004847:Epithelial Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D004926:Escherichia coli / Q000737:chemistry*; D006801:Humans; D042541:Intracellular Space / Q000187:drug effects / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology*; D019804:Mesalamine / Q000378:metabolism*; D010539:Permeability / Q000187:drug effects; D019936:Probiotics / Q000737:chemistry*
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D017077:Culture Media, Conditioned; D008070:Lipopolysaccharides; D019804:Mesalamine