title: The carbon storage regulator (Csr) system exerts a nutrient-specific control over central metabolism in Escherichia coli strain Nissle 1917.
abstract: The role of the post-transcriptional carbon storage regulator (Csr) system in nutrient utilization and in the control of the central metabolism in E. coli reference commensal strain Nissle 1917 was investigated. Analysis of the growth capabilities of mutants altered for various components of the Csr system (csrA51, csrB, csrC and csrD mutations) showed that only the protein CsrA - the key component of the system - exerts a marked role in carbon nutrition. Attenuation of CsrA activity in the csrA51 mutant affects the growth efficiency on a broad range of physiologically relevant carbon sources, including compounds utilized by the Entner-Doudoroff (ED) pathway. Detailed investigations of the metabolomes and fluxomes of mutants and wild-type cells grown on carbon sources representative of glycolysis and of the ED pathway (glucose and gluconate, respectively), revealed significant re-adjusting of central carbon metabolism for both compounds in the csrA51 mutant. However, the metabolic re-adjusting observed on gluconate was strikingly different from that observed on glucose, indicating a nutrient-specific control of metabolism by the Csr system.
authors.affiliation: Laboratoire d'Ingénierie des Systèmes Biologiques et des Procédés, LISBP, Université de Toulouse, INSA, UPS, INP, Toulouse, France.
authors.forename: Olga
authors.initials: O
authors.lastname: Revelles
authors.forename: Pierre
authors.initials: P
authors.lastname: Millard
authors.forename: Jean-Philippe
authors.initials: JP
authors.lastname: Nougayrède
authors.forename: Ulrich
authors.initials: U
authors.lastname: Dobrindt
authors.forename: Eric
authors.initials: E
authors.lastname: Oswald
authors.forename: Fabien
authors.initials: F
authors.lastname: Létisse
authors.forename: Jean-Charles
authors.initials: JC
authors.lastname: Portais
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D050260:Carbohydrate Metabolism; D003470:Culture Media; D004926:Escherichia coli / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial; D055786:Gene Knockout Techniques; D005942:Gluconates / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D006003:Glycogen / Q000378:metabolism; D053858:Metabolic Networks and Pathways; D009249:NADP / Q000378:metabolism; D062085:RNA, Long Noncoding / Q000235:genetics; D016601:RNA-Binding Proteins / Q000235:genetics*; D012097:Repressor Proteins / Q000235:genetics*
chemical_list: C093587:CsrA protein, E coli; C107123:CsrB RNA, E coli; D003470:Culture Media; D029968:Escherichia coli Proteins; D005942:Gluconates; D062085:RNA, Long Noncoding; D016601:RNA-Binding Proteins; D012097:Repressor Proteins; D009249:NADP; D000255:Adenosine Triphosphate; D006003:Glycogen; D005947:Glucose; C030691:gluconic acid