title: Isolation, characterization and acetylcholinesterase inhibitory activity of alkaloids from roots of Stemona sessilifolia.
abstract: Two new alkaloids, named stenine A (1) and stenine B (2), along with the known compounds neostenine (3), stenine (4) and neotuberostemonine (5), were isolated from the roots of Stemona sessilifolia. Their structures were elucidated by 1D- and 2D-NMR spectra and X-ray single-crystal diffraction experiment. Anti-acetylcholinesterase (AChE) activity of compounds 1-5 were also tested. Compounds 2 and 4 showed significant anti-acetylcholinesterase activities, with IC50 values of 2.1±0.2 μM and 19.8±2.5 μM, resp. The mode of AChE inhibition by Compound 2 (the most potential AChE inhibitor) was reversible and competitive. In addition, molecular modeling was performed to explore the binding mode of compound 2 with AChE.
authors.affiliation: School of Life Science and Engineering, Lanzhou University of Technology, Lanzhou 730050, PR China.
authors.forename: Dong-Hai
authors.initials: DH
authors.lastname: Lai
authors.forename: Zhong-Duo
authors.initials: ZD
authors.lastname: Yang
authors.forename: Wei-Wei
authors.initials: WW
authors.lastname: Xue
authors.forename: Jie
authors.initials: J
authors.lastname: Sheng
authors.forename: Yin
authors.initials: Y
authors.lastname: Shi
authors.forename: Xiao-Jun
authors.initials: XJ
authors.lastname: Yao
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism*; D000470:Alkaloids / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D002800:Cholinesterase Inhibitors / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D020128:Inhibitory Concentration 50; D008958:Models, Molecular; D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D018517:Plant Roots / Q000737:chemistry; D041901:Stemonaceae / Q000737:chemistry*
chemical_list: D000470:Alkaloids; D002800:Cholinesterase Inhibitors; D010936:Plant Extracts; C441784:stenine; D000110:Acetylcholinesterase
keywords: Acetylcholinesterase inhibitors; Alkaloids; Alzheimer's disease; Stemona sessilifolia