title: Structure of peroxiredoxin from the anaerobic hyperthermophilic archaeon Pyrococcus horikoshii.
abstract: The crystal structure of peroxiredoxin from the anaerobic hyperthermophilic archaeon Pyrococcus horikoshii (PhPrx) was determined at a resolution of 2.25 Å. The overall structure was a ring-type decamer consisting of five homodimers. Citrate, which was included in the crystallization conditions, was bound to the peroxidatic cysteine of the active site, with two O atoms of the carboxyl group mimicking those of the substrate hydrogen peroxide. PhPrx lacked the C-terminal tail that forms a 32-residue extension of the protein in the homologous peroxiredoxin from Aeropyrum pernix (ApPrx).
authors.affiliation: National Institute of Advanced Industrial Science and Technology, Ikeda, Osaka 563-8577, Japan. nakamura-t@aist.go.jp
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Nakamura
authors.forename: Aika
authors.initials: A
authors.lastname: Mori
authors.forename: Mayumi
authors.initials: M
authors.lastname: Niiyama
authors.forename: Hiroyoshi
authors.initials: H
authors.lastname: Matsumura
authors.forename: Chisa
authors.initials: C
authors.lastname: Tokuyama
authors.forename: Junji
authors.initials: J
authors.lastname: Morita
authors.forename: Koichi
authors.initials: K
authors.lastname: Uegaki
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Inoue
mesh_terms: D041522:Aeropyrum / Q000378:metabolism; D000595:Amino Acid Sequence; D001105:Archaea / Q000378:metabolism; D001665:Binding Sites; D019343:Citric Acid / Q000737:chemistry / Q000378:metabolism*; D003460:Crystallization; D018360:Crystallography, X-Ray; D003545:Cysteine / Q000737:chemistry / Q000378:metabolism*; D006861:Hydrogen Peroxide / Q000737:chemistry; D008958:Models, Molecular; D008969:Molecular Sequence Data; D016877:Oxidants / Q000737:chemistry; D054464:Peroxiredoxins / Q000737:chemistry* / Q000378:metabolism; D011487:Protein Conformation; D043902:Pyrococcus horikoshii / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid
chemical_list: D016877:Oxidants; D019343:Citric Acid; D006861:Hydrogen Peroxide; D054464:Peroxiredoxins; D003545:Cysteine
keywords: Pyrococcus horikoshii; peroxiredoxins