title: Extracellular matrix of secondary lymphoid organs impacts on B-cell fate and survival.
abstract: We describe a unique extracellular matrix (ECM) niche in the spleen, the marginal zone (MZ), characterized by the basement membrane glycoproteins, laminin α5 and agrin, that promotes formation of a specialized population of MZ B lymphocytes that respond rapidly to blood-borne antigens. Mice with reduced laminin α5 expression show reduced MZ B cells and increased numbers of newly formed (NF) transitional B cells that migrate from the bone marrow, without changes in other immune or stromal cell compartments. Transient integrin α6β1-mediated interaction of NF B cells with laminin α5 in the MZ supports the MZ B-cell population, their long-term survival, and antibody response. Data suggest that the unique 3D structure and biochemical composition of the ECM of lymphoid organs impacts on immune cell fate.
authors.affiliation: Institute of Physiological Chemistry and Pathobiochemistry, Muenster University, 48149 Muenster, Germany.
authors.forename: Jian
authors.initials: J
authors.lastname: Song
authors.forename: Zerina
authors.initials: Z
authors.lastname: Lokmic
authors.forename: Tim
authors.initials: T
authors.lastname: Lämmermann
authors.forename: Julia
authors.initials: J
authors.lastname: Rolf
authors.forename: Chuan
authors.initials: C
authors.lastname: Wu
authors.forename: Xueli
authors.initials: X
authors.lastname: Zhang
authors.forename: Rupert
authors.initials: R
authors.lastname: Hallmann
authors.forename: Melanie-Jane
authors.initials: MJ
authors.lastname: Hannocks
authors.forename: Nathalie
authors.initials: N
authors.lastname: Horn
authors.forename: Markus A
authors.initials: MA
authors.lastname: Ruegg
authors.forename: Arnoud
authors.initials: A
authors.lastname: Sonnenberg
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Georges-Labouesse
authors.forename: Thomas H
authors.initials: TH
authors.lastname: Winkler
authors.forename: John F
authors.initials: JF
authors.lastname: Kearney
authors.forename: Susanna
authors.initials: S
authors.lastname: Cardell
authors.forename: Lydia
authors.initials: L
authors.lastname: Sorokin
mesh_terms: D018171:Agrin / Q000235:genetics / Q000276:immunology; D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology*; D001853:Bone Marrow / Q000276:immunology*; D002465:Cell Movement / Q000235:genetics / Q000276:immunology*; D002470:Cell Survival / Q000235:genetics / Q000276:immunology; D005109:Extracellular Matrix / Q000235:genetics / Q000276:immunology*; D039121:Integrin alpha6beta1 / Q000235:genetics / Q000276:immunology; D007797:Laminin / Q000235:genetics / Q000276:immunology; D051379:Mice; D018345:Mice, Knockout; D013154:Spleen / Q000166:cytology / Q000276:immunology*
chemical_list: D018171:Agrin; D039121:Integrin alpha6beta1; D007797:Laminin; C097043:laminin alpha5
keywords: B-cell development; immunology