title: The effect of low dose teicoplanin-loaded acrylic bone cement on biocompatibility of bone cement.
abstract: Antibiotic-loaded acrylic bone cement (polymethylmethacrylate, PMMA) is used to prevent or treat infection in total joint replacement surgery. The purpose of this study was to investigate biocompatibility and cytotoxicity of the teicoplanin-loaded acrylic bone cement. Cytotoxicity examination of acrylic bone cement balls and 400 mg teicoplanin added acrylic bone cement balls conducted by MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) assay. SEM (Scanning electron microscopy) was used to observe adhesion and spreading of cells on surface of the balls. Cytotoxicity examination conducted by MTT assay on acrylic bone cement balls and teicoplanin-added acrylic bone cement balls revealed no cytotoxicity. SEM analysis put forward that cells started to proliferate and adhere on surface of the samples in both groups as a result of 48-hour incubation and that the cell proliferation over acrylic bone cement and teicoplanin-added acrylic bone cement was similar. As a consequence, there was no cytotoxicity in acrylic bone cement and teicoplanin-added acrylic bone cement groups according to results of MTT assay. On the other hand, results of SEM showed that biocompatibility of both groups was similar. In conclusion, teicoplanin-loaded bone cement did not change biocompatibility of bone cement in studied dose.
authors.affiliation: Cumhuriyet University School of Medicine, Department of Orthopaedic and Traumatology, Sivas, Turkey. oztemur@cumhuriyet.edu.tr
authors.forename: Zekeriya
authors.initials: Z
authors.lastname: Öztemür
authors.forename: Zeynep
authors.initials: Z
authors.lastname: Sümer
authors.forename: Tutku
authors.initials: T
authors.lastname: Tunç
authors.forename: Özhan
authors.initials: Ö
authors.lastname: Pazarcé
authors.forename: Okay
authors.initials: O
authors.lastname: Bulut
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D001672:Biocompatible Materials / Q000737:chemistry*; D001843:Bone Cements / Q000737:chemistry* / Q000633:toxicity; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004337:Drug Carriers / Q000737:chemistry* / Q000633:toxicity; D006801:Humans; D051379:Mice; D019904:Polymethyl Methacrylate / Q000737:chemistry* / Q000633:toxicity; D017334:Teicoplanin / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D001672:Biocompatible Materials; D001843:Bone Cements; D004337:Drug Carriers; D017334:Teicoplanin; D019904:Polymethyl Methacrylate