title: Overexpression of AtALMT1 in the Arabidopsis thaliana ecotype Columbia results in enhanced Al-activated malate excretion and beneficial bacterium recruitment.
abstract: AtALMT1 (Arabidopsis thaliana ALuminum activated Malate Transporter 1) encodes an Arabidopsis thaliana malate transporter that has a pleiotropic role in Arabidopsis stress tolerance. Malate released through AtALMT1 protects the root tip from Al rhizotoxicity, and recruits beneficial rhizobacteria that induce plant immunity. To examine whether the overexpression of AtALMT1 can improve these traits, the gene, driven by the cauliflower mosaic virus 35S promoter, was introduced into the Arabidopsis ecotype Columbia. Overexpression of the gene enhanced both Al-activated malate excretion and the recruitment of beneficial bacteria Bacillus subtilis strain FB17. These findings suggest that overexpression of AtALMT1 can be used as an approach to enhance a plant's ability to release malate into the rhizosphere, which can enhance plant tolerance to some environmental stress factors.
authors.affiliation: Laboratory of Plant Cell Technology; Faculty of Applied Biological Sciences; Gifu University; Gifu, Japan.
authors.forename: Yasufumi
authors.initials: Y
authors.lastname: Kobayashi
authors.forename: Venkatachalam
authors.initials: V
authors.lastname: Lakshmanan
authors.forename: Yuriko
authors.initials: Y
authors.lastname: Kobayashi
authors.forename: Minatsu
authors.initials: M
authors.lastname: Asai
authors.forename: Satoshi
authors.initials: S
authors.lastname: Iuchi
authors.forename: Masatomo
authors.initials: M
authors.lastname: Kobayashi
authors.forename: Harsh P
authors.initials: HP
authors.lastname: Bais
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Koyama
mesh_terms: D000535:Aluminum / Q000633:toxicity*; D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics / Q000378:metabolism* / Q000382:microbiology*; D029681:Arabidopsis Proteins / Q000378:metabolism*; D001412:Bacillus subtilis / Q000187:drug effects / Q000502:physiology*; D060146:Ecotype; D008293:Malates / Q000378:metabolism*; D027361:Organic Anion Transporters / Q000378:metabolism*; D010641:Phenotype; D030821:Plants, Genetically Modified
chemical_list: C512361:ALMT1 protein, Arabidopsis; D029681:Arabidopsis Proteins; D008293:Malates; D027361:Organic Anion Transporters; C030298:malic acid; D000535:Aluminum
keywords: Arabidopsis thaliana; AtALMT1; Bacillus subtilisstrain FB17; Pseudomonas syringaepv tomatoDC3000; aluminum; malate exudation