title: Separation of carbohydrates using hydrophilic interaction liquid chromatography.
abstract: A strategy was developed to rapidly evaluate chromatographic properties of hydrophilic interaction chromatography (HILIC) columns for separating carbohydrates. Seven HILIC columns (Silica, Diol, TSK Amide-80, XAmide, Click Maltose, Click β-CD, and Click TE-Cys columns) were evaluated by using three monosaccharide and seven disaccharides as probes. The influence of column temperature on the peak shape and tautomerization of carbohydrates, as well as column selectivity were investigated. The influence of surface charge property on the retention was also studied by using glucose, glucuronic acid, and glucosamine, which indicated that buffer salt concentration and pH value in mobile phase was necessary to control the ionic interactions between ionic carbohydrates and HILIC columns. According to evaluation results, the XAmide column was selected as an example to establish experimental schemes for separation of complex mixtures of oligosaccharide.
authors.affiliation: Engineering Research Center of Pharmaceutical Process Chemistry, Ministry of Education, School of Pharmacy, East China University of Science and Technology, Shanghai 200237, PR China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Fu
authors.forename: Tu
authors.initials: T
authors.lastname: Liang
authors.forename: Zhenyu
authors.initials: Z
authors.lastname: Li
authors.forename: Xiaoyong
authors.initials: X
authors.lastname: Xu
authors.forename: Yanxiong
authors.initials: Y
authors.lastname: Ke
authors.forename: Yu
authors.initials: Y
authors.lastname: Jin
authors.forename: Xinmiao
authors.initials: X
authors.lastname: Liang
mesh_terms: D002241:Carbohydrates / Q000737:chemistry* / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D057927:Hydrophobic and Hydrophilic Interactions; D015394:Molecular Structure; D013499:Surface Properties; D013696:Temperature
chemical_list: D002241:Carbohydrates
keywords: Carbohydrates; Chromatographic characterization; Hydrophilic interaction liquid chromatography; Selectivity; Stationary phase