title: A new comprehensive procedure for the quality control of Semen Cassiae and its application in evaluating commercially available material in China.
abstract: OBJECTIVE
  To establish a more comprehensive and suitable procedure for the quality control of Semen Cassiae which can be used to supplement the evaluation procedure adopted by the Pharmacopoeia of the Peoples Republic of China.
  
  
  METHODS
  A HPLC assay-based comprehensive quality evaluation procedure for Semen Cassiae using three bioactive compounds including anthraquinones and naphthopyrones, i.e., chrysophanol-1-O-β-D-glucopyranosyl-(1→3)-β-D-glucopyranosyl-(1→6)-β-D-glucopyranoside (1), rubrofusarin-6-O-β-D-gentiobioside (2) and toralactone-9-O-β-D-gentiobioside (3) as the index components was established. The resultant data were further analyzed by principal component analysis (PCA) and data distribution methods using software SPSS 16.0.
  
  
  RESULTS
  Sixty-six batches of Semen Cassiae obtained from various regions of China were analyzed with the procedure. Based on the test results of these batches, the content limits of the three bioactive compounds in Semen Cassiae were proposed.
  
  
  CONCLUSIONS
  The procedure established herein is more comprehensive and appropriate for the quality evaluation of Semen Cassiae commercially available in China, and can be used as a useful supplement to the current official method in China for the quality evaluation of Semen Cassiae.
authors.affiliation: School of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100102, China.
authors.forename: Yu-Lin
authors.initials: YL
authors.lastname: Diao
authors.forename: Ru-Feng
authors.initials: RF
authors.lastname: Wang
authors.forename: Chen
authors.initials: C
authors.lastname: Zhang
authors.forename: Jian-Xin
authors.initials: JX
authors.lastname: Chen
authors.forename: Bin
authors.initials: B
authors.lastname: Liu
mesh_terms: D002681:China; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D032904:Cinnamomum aromaticum / Q000737:chemistry*; D004365:Drugs, Chinese Herbal / Q000032:analysis* / Q000191:economics / Q000592:standards; D025341:Principal Component Analysis; D011786:Quality Control; D012984:Software
chemical_list: D004365:Drugs, Chinese Herbal
keywords: Anthraquinones; Naphthopyrones; PCA analysis; Quality evaluation; RP-HPLC; Semen Cassiae