title: Mutation of a single amino acid converts the human water channel aquaporin 5 into an anion channel.
abstract: Aquaporin 6 (AQP6) is unique among mammalian AQPs in being an anion channel with negligible water permeability. However, the point mutation Asn60Gly converts AQP6 from an anion channel into a water channel. In the present study of human AQP5, we mutated Leu51 (corresponding to residue 61 in AQP6), the side chain of which faces the central pore. We evaluated function in Xenopus oocytes by two-electrode voltage clamp, video measurements of osmotic H2O permeability (Pf), microelectrode measurements of surface pH (pHS) to assess CO2 permeability, and surface biotinylation. We found that AQP5-L51R does not exhibit the H2O or CO2 permeability of the wild-type protein but instead has a novel p-chloromercuribenzene sulfonate (pCMBS)-sensitive current. The double mutant AQP5-L51R/C182S renders the conductance insensitive to pCMBS, demonstrating that the current is intrinsic to AQP5. AQP5-L51R has the anion permeability sequence I(-) > NO3(-) ≅ NO2(-) > Br(-) > Cl(-) > HCO3(-) > gluconate. Of the other L51 mutants, L51T (polar uncharged) and L51V (nonpolar) retain H2O and CO2 permeability and do not exhibit anion conductance. L51D and L51E (negatively charged) have no H2O or CO2 permeability. L51K (positively charged) has an intermediate H2O and CO2 permeability and anion conductance. L51H is unusual in having a relatively low CO2 permeability and anion conductance, but a moderate Pf. Thus, positively charged mutations of L51 can convert AQP5 from a H2O/CO2 channel into an anion channel. However, the paradoxical effect of L51H is consistent with the hypothesis that CO2, in part, takes a pathway different from H2O through AQP5.
authors.affiliation: Department of Physiology and Biophysics, Case Western Reserve University School of Medicine, Cleveland, Ohio.
authors.forename: Xue
authors.initials: X
authors.lastname: Qin
authors.forename: Walter F
authors.initials: WF
authors.lastname: Boron
mesh_terms: D019943:Amino Acid Substitution; D000818:Animals; D000838:Anions / Q000378:metabolism; D051402:Aquaporin 5 / Q000235:genetics* / Q000378:metabolism*; D002245:Carbon Dioxide / Q000378:metabolism; D002462:Cell Membrane / Q000235:genetics / Q000378:metabolism; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007473:Ion Channels / Q000235:genetics* / Q000378:metabolism*; D007930:Leucine / Q000235:genetics / Q000378:metabolism; D009865:Oocytes / Q000378:metabolism; D010539:Permeability; D017354:Point Mutation; D014867:Water / Q000378:metabolism*; D014982:Xenopus laevis
chemical_list: C496036:AQP5 protein, human; D000838:Anions; D051402:Aquaporin 5; D007473:Ion Channels; D014867:Water; D002245:Carbon Dioxide; D007930:Leucine
keywords: anion channel; aquaporin 5; gas channels; gas transport; water transport