title: O-linked mannose β-1,2-N-acetylglucosaminyltransferase 1 correlated with the malignancy in glioma.
abstract: O-linked mannose β-1,2-N-acetylglucosaminyltransferase 1 (PomGnT1) constitutes one third of the O-linked glycoproteins in brain tissue. However, its functions have been seldom investigated in brain cancers. In this study, immunohistochemistry was used for the detection of the PomGnT1 protein in 133 cases of glioma tissues. Spearman correlation analysis was used for the relationship between PomGnT1 staining and the glioma grade. Receiver operating characteristic curve was used to measure the diagnostic value of PomGnT1 protein in the degree of glioma malignance. We found that PomGnT1 expression was correlated with glioma grade, and it could be used as a marker to distinguish low- and high-grade gliomas. Stably transfected U87 cells were constructed to overexpress short hairpin RNA of PomGnT1. Immunofluorescence test detected that this protein also could restrain the generation of U87 cells' pseudopodia. Western blotting further showed that the PomGnT1 protein had an impact on the c-myc protein level. In conclusion, our data suggest that PomGnT1 protein was correlated with the malignance of glioma progression, the mechanism involved in glioma cell's pseudopodium formation, and the expression of c-myc protein.
authors.affiliation: Department of Neurosurgery, Ren Ji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Lan
authors.forename: Pin
authors.initials: P
authors.lastname: Guo
authors.forename: Mo
authors.initials: M
authors.lastname: Chen
authors.forename: Bin
authors.initials: B
authors.lastname: Wu
authors.forename: Qing
authors.initials: Q
authors.lastname: Mao
authors.forename: Yongming
authors.initials: Y
authors.lastname: Qiu
mesh_terms: D014408:Biomarkers, Tumor / Q000032:analysis; D001932:Brain Neoplasms / Q000145:classification / Q000201:enzymology* / Q000473:pathology; D045744:Cell Line, Tumor; D015971:Gene Expression Regulation, Enzymologic / Q000235:genetics; D020868:Gene Silencing; D005910:Glioma / Q000145:classification / Q000201:enzymology* / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D017351:N-Acetylglucosaminyltransferases / Q000032:analysis*; D060787:Neoplasm Grading; D009361:Neoplasm Invasiveness; D016271:Proto-Oncogene Proteins c-myc / Q000032:analysis; D011554:Pseudopodia / Q000201:enzymology; D034741:RNA, Small Interfering / Q000235:genetics; D012372:ROC Curve; D014162:Transfection
chemical_list: D014408:Biomarkers, Tumor; C489427:MYC protein, human; D016271:Proto-Oncogene Proteins c-myc; D034741:RNA, Small Interfering; D017351:N-Acetylglucosaminyltransferases; C443106:protein O-mannose beta-1,2-N-acetylglucosaminyltransferase