title: Lepista sordida polysaccharide induces apoptosis of Hep-2 cancer cells via mitochondrial pathway.
abstract: In our previous study, a potential antitumor polysaccharide (LSPc1) was screened from the fruiting bodies of Lepista sordida. However, its molecular mechanism of cell death induction on Hep-2 human laryngocarcinoma cells has still not been determined. The present study evaluated the anticancer efficacy and associated mechanisms of LSPc1 on Hep-2 cells in vitro. We found that LSPc1-induced inhibition of cell proliferation was associated with an increase in G2/M-phase arrest at 48 h. Typical morphological and biochemical features of apoptosis were also observed in LSPc1-treated cells. Furthermore, LSPc1 led a loss of mitochondrial membrane potential (ΔΨm), increased the ratio of pro-apoptotic Bax to anti-apoptotic Bcl-2, induced cytochrome c release, and increased the activity of caspase-3 and -9, which altogether account for apoptotic cell death. Taken together, our study suggests that intrinsic mitochondrial caspase dependent pathway plays a very important role in LSPc1-induced cancer apoptosis and these results provided strong experimental evidence for the use of LSPc1 as a potential therapeutic agent in cancer for laryngocarcinoma.
authors.affiliation: Department of Head and Neck Surgery, The Third Affiliated Hospital of Harbin Medical University, Haping Road 150, 150081 Harbin, China.
authors.forename: Susheng
authors.initials: S
authors.lastname: Miao
authors.forename: Xionghui
authors.initials: X
authors.lastname: Mao
authors.forename: Rong
authors.initials: R
authors.lastname: Pei
authors.forename: Suping
authors.initials: S
authors.lastname: Miao
authors.forename: Cheng
authors.initials: C
authors.lastname: Xiang
authors.forename: Yuanjing
authors.initials: Y
authors.lastname: Lv
authors.forename: Xianguang
authors.initials: X
authors.lastname: Yang
authors.forename: Ji
authors.initials: J
authors.lastname: Sun
authors.forename: Shenshan
authors.initials: S
authors.lastname: Jia
authors.forename: Yvpeng
authors.initials: Y
authors.lastname: Liu
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D001487:Basidiomycota / Q000737:chemistry*; D020169:Caspases / Q000378:metabolism; D059447:Cell Cycle Checkpoints / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D045304:Cytochromes c / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004789:Enzyme Activation / Q000187:drug effects; D062610:Fungal Polysaccharides / Q000737:chemistry / Q000494:pharmacology*; D056945:Hep G2 Cells; D006801:Humans; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D008928:Mitochondria / Q000187:drug effects* / Q000378:metabolism*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects*
chemical_list: D062610:Fungal Polysaccharides; D019253:Proto-Oncogene Proteins c-bcl-2; D045304:Cytochromes c; D020169:Caspases
keywords: Apoptosis; Laryngocarcinoma; Lepista sordida; Mitochondrial pathway; Polysaccharide