title: Influence of physical properties of cuvette surface on measurement of serum lipase.
abstract: BACKGROUND
  Despite striking similarities among colorimetric lipase assay recipes, marked intervendor differences are noted in the reported lipase values. In the present study, the effect of physical properties of the cuvette surface on measurement of serum lipase was investigated.
  
  
  METHODS
  Lipase activity was measured concomitantly in cuvettes from three different analyzers: Vista (Siemens), Modular (Roche), and Synchron (Beckman Coulter). The surface/volume ratio of the cuvettes and the contact angle of the cuvette polymers were determined. The effects of various characteristics of serum (biochemical parameters, surface tension) were also examined.
  
  
  RESULTS
  Serum lipase activities based on the colorimetric methylresorufin assay differed markedly according to the cuvettes used. More specifically, in the lower activity rate, marked differences were reported. The physical properties of the various cuvettes showed remarkable differences, especially in the contact angles. Other biochemical parameters (bilirubin, alkaline phosphatase, total cholesterol, high-density lipoprotein cholesterol, low-density lipoprotein cholesterol, and triglycerides) and serum surface tension did not affect the results.
  
  
  CONCLUSIONS
  Serum lipase activity is affected by the physical properties of the cuvette surface.
authors.forename: Lien
authors.initials: L
authors.lastname: Cattoir
authors.forename: Valerie
authors.initials: V
authors.lastname: Pede
authors.forename: An-Sofie
authors.initials: AS
authors.lastname: Decavele
authors.forename: Marc L
authors.initials: ML
authors.lastname: De Buyzere
authors.forename: Marijn M
authors.initials: MM
authors.lastname: Speeckaert
authors.forename: David
authors.initials: D
authors.lastname: Seccombe
authors.forename: Veerle
authors.initials: V
authors.lastname: Boterberg
authors.forename: Peter
authors.initials: P
authors.lastname: Dubruel
authors.forename: Frank
authors.initials: F
authors.lastname: de Bisschop
authors.forename: Joris R
authors.initials: JR
authors.lastname: Delanghe
mesh_terms: D016477:Artifacts; D001774:Blood Chemical Analysis / Q000295:instrumentation*; D057075:Enzyme Assays; D006801:Humans; D006868:Hydrolysis; D008049:Lipase / Q000097:blood* / Q000378:metabolism; D055585:Physical Phenomena; D012044:Regression Analysis; D013056:Spectrophotometry, Ultraviolet; D013500:Surface Tension
chemical_list: D008049:Lipase