title: Rapid diagnosis of liver fibrosis using multimodal multiphoton nonlinear optical microspectroscopy imaging.
abstract: A multimodal multiphoton nonlinear optical (NLO) microspectroscopy imaging system was developed using a femtosecond laser and a photonic crystal fiber. Coherent anti-Stokes Raman scattering (CARS) microspectroscopy was combined with two-photon excitation fluorescence and second-harmonic generation microscopy in one platform and the system was applied to diagnose liver fibrosis. Normal and liver fibrosis tissues were clearly distinguished with the great difference from CARS spectra as well as multimodal multiphoton NLO images. We expect the system to be a rapid diagnosis tool for liver fibrosis at tissue level with label-free imaging of significant biochemical components.
authors.affiliation: Gwangju Institute of Science and Technology, Department of Physics and Photon Science, Gwangju 500-712, Republic of Korea.
authors.forename: Jang Hyuk
authors.initials: JH
authors.lastname: Lee
authors.forename: Jong Chul
authors.initials: JC
authors.lastname: Kim
authors.forename: Giyoong
authors.initials: G
authors.lastname: Tae
authors.forename: Myoung-Kyu
authors.initials: MK
authors.lastname: Oh
authors.forename: Do-Kyeong
authors.initials: DK
authors.lastname: Ko
mesh_terms: D000818:Animals; D005260:Female; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D008099:Liver / Q000473:pathology; D008103:Liver Cirrhosis / Q000175:diagnosis* / Q000473:pathology*; D051379:Mice; D008807:Mice, Inbred BALB C; D036641:Microscopy, Fluorescence, Multiphoton / Q000379:methods*; D015591:Microspectrophotometry / Q000379:methods*; D013059:Spectrum Analysis, Raman