title: Multifunctional nanovesicle-bioactive conjugates prepared by a one-step scalable method using CO2-expanded solvents.
abstract: The integration of therapeutic biomolecules, such as proteins and peptides, in nanovesicles is a widely used strategy to improve their stability and efficacy. However, the translation of these promising nanotherapeutics to clinical tests is still challenged by the complexity involved in the preparation of functional nanovesicles and their reproducibility, scalability, and cost production. Here we introduce a simple one-step methodology based on the use of CO2-expanded solvents to prepare multifunctional nanovesicle-bioactive conjugates. We demonstrate high vesicle-to-vesicle homogeneity in terms of size and lamellarity, batch-to-batch consistency, and reproducibility upon scaling-up. Importantly, the procedure is readily amenable to the integration/encapsulation of multiple components into the nanovesicles in a single step and yields sufficient quantities for clinical research. The simplicity, reproducibility, and scalability render this one-step fabrication process ideal for the rapid and low-cost translation of nanomedicine candidates from the bench to the clinic.
authors.affiliation: Institut de Ciència de Materials de Barcelona (ICMAB-CSIC), Campus Universitari de Bellaterra, 08193 Cerdanyola del Vallès, Spain.
authors.forename: Ingrid
authors.initials: I
authors.lastname: Cabrera
authors.forename: Elisa
authors.initials: E
authors.lastname: Elizondo
authors.forename: Olga
authors.initials: O
authors.lastname: Esteban
authors.forename: José Luis
authors.initials: JL
authors.lastname: Corchero
authors.forename: Marta
authors.initials: M
authors.lastname: Melgarejo
authors.forename: Daniel
authors.initials: D
authors.lastname: Pulido
authors.forename: Alba
authors.initials: A
authors.lastname: Córdoba
authors.forename: Evelyn
authors.initials: E
authors.lastname: Moreno
authors.forename: Ugutz
authors.initials: U
authors.lastname: Unzueta
authors.forename: Esther
authors.initials: E
authors.lastname: Vazquez
authors.forename: Ibane
authors.initials: I
authors.lastname: Abasolo
authors.forename: Simó
authors.initials: S
authors.lastname: Schwartz
authors.forename: Antonio
authors.initials: A
authors.lastname: Villaverde
authors.forename: Fernando
authors.initials: F
authors.lastname: Albericio
authors.forename: Miriam
authors.initials: M
authors.lastname: Royo
authors.forename: Maria F
authors.initials: MF
authors.lastname: García-Parajo
authors.forename: Nora
authors.initials: N
authors.lastname: Ventosa
authors.forename: Jaume
authors.initials: J
authors.lastname: Veciana
mesh_terms: D000818:Animals; D002245:Carbon Dioxide / Q000737:chemistry*; D002417:Cattle; D002460:Cell Line; D049452:Green Fluorescent Proteins / Q000737:chemistry*; D006801:Humans; D015394:Molecular Structure; D049329:Nanostructures / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D012997:Solvents / Q000737:chemistry
chemical_list: D012997:Solvents; D002245:Carbon Dioxide; D049452:Green Fluorescent Proteins; D012710:Serum Albumin, Bovine; D011092:Polyethylene Glycols