title: Preparation and properties of nanometer silk fibroin peptide/polyvinyl alcohol blend films for cell growth.
abstract: Nanometer silk fibroin peptide (Nano-SFP) was prepared from silkworm cocoons through the process of dissolution, dialysis and enzymolysis. For comparison, silk fibroin was decomposed with α-chymotrypsin, trypsin and neutrase, respectively. From the SEM and particle size analysis results, the Nano-SFP prepared by neutrase was found to be the most desirable at about 50-200 nm. Nano-SFP/polyvinyl alcohol films (Nano-SFP/PVA) were prepared by blending Nano-SFP and PVA in water with different weight ratios of 10/90, 20/80, 30/70, and 40/60. The films were characterized by IR, SEM, TG, DSC and tensile strength test for investigating their structure, surface morphology, thermostability, and mechanical property. The results showed that Nano-SFP inserted in the PVA films with small linear particles, and Nano-SFP/PVA films exhibited smooth surface, good thermostability and tensile strength. The growth of Chinese hamster ovary (CHO) cells on films with and without Nano-SFP was investigated with MTT colorimetric assay to assess the films' ability to promote cell growth. It was observed that the Nano-SFP improved cell adhesion on the film surface, and the ability of promoting cell growth increased with the increasing content of Nano-SFP in the blend films. Nano-SFP/PVA film with the ratio of 30/70 was concluded to have the best properties.
authors.affiliation: College of Pharmacy and Biomedical Engineering, Chongqing University of Technology, No. 69 Hongguang Road, Ba'nan District, Chongqing 400054, PR China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Luo
authors.forename: Zhongmin
authors.initials: Z
authors.lastname: Chen
authors.forename: Xuefei
authors.initials: X
authors.lastname: Hao
authors.forename: Qiangsong
authors.initials: Q
authors.lastname: Zhu
authors.forename: Yucheng
authors.initials: Y
authors.lastname: Zhou
mesh_terms: D000818:Animals; D001696:Biomechanical Phenomena; D016466:CHO Cells; D018929:Cell Culture Techniques; D006224:Cricetinae; D003412:Cricetulus; D005349:Fibroins / Q000737:chemistry*; D057139:Nanofibers / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size; D010455:Peptides / Q000737:chemistry*; D011142:Polyvinyl Alcohol / Q000737:chemistry*; D047011:Silk / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D013816:Thermodynamics
chemical_list: D010455:Peptides; D047011:Silk; D011142:Polyvinyl Alcohol; D005349:Fibroins
keywords: Blend films; Cell growth; Nanometer; Polyvinyl alcohol; Silk fibroin peptide