title: Can garlic oil ameliorate diabetes-induced oxidative stress in a rat liver model? A correlated histological and biochemical study.
abstract: This study aimed to characterise the structural changes in liver of an alloxan-induced diabetic rat and to explain such changes in terms of the biochemical changes in free radicals and antioxidants. In addition, it aimed to determine the potential ability of garlic oil to alter these changes. The study groups were: control (n=12), alloxan-induced diabetic rats (n=10) and alloxan-induced diabetic rats treated with garlic oil (10 mg/kg body weight (n=10)). Markers of oxidative stress were assessed. Small pieces of the liver were processed for transmission electron microscopic study. Garlic oil caused a significant decrease in levels of LPO in plasma (0.26 vs 0.53), erythrocyte lysate (14.4 vs 24.8) and liver tissue homogenate (1.04 vs 2.08), whereas those of thiols were significantly elevated (1.2 vs 0.46), (24 vs 15) in plasma and erythrocyte lysate respectively. SOD activity and G-S-T activity were significantly elevated in erythrocyte lysate (5.7 vs 3.3) (377 vs 179) and liver homogenate (1.4 vs 0.5) (752 vs 623) respectively after garlic oil administration. Ultrastructural study of the liver confirmed the ability of garlic to retard lipid peroxidation of cellular membranes induced by oxidative stress associated with diabetes. Therefore, garlic could normalise oxidative stress in alloxan-induced diabetic rats.
authors.affiliation: Department of Zoology, Faculty of Sciences, Assuit University, Egypt.
authors.forename: Hanem Saad
authors.initials: HS
authors.lastname: Abdultawab
authors.forename: Nasra N
authors.initials: NN
authors.lastname: Ayuob
mesh_terms: D000498:Allyl Compounds / Q000008:administration & dosage / Q000627:therapeutic use; D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage / Q000627:therapeutic use*; D015415:Biomarkers / Q000097:blood / Q000378:metabolism; D003920:Diabetes Mellitus / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D004912:Erythrocytes / Q000187:drug effects / Q000378:metabolism; D005737:Garlic / Q000737:chemistry*; D006949:Hyperlipidemias / Q000209:etiology / Q000517:prevention & control; D007004:Hypoglycemic Agents / Q000008:administration & dosage / Q000627:therapeutic use; D000960:Hypolipidemic Agents / Q000008:administration & dosage / Q000627:therapeutic use; D007274:Injections, Intraperitoneal; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D008297:Male; D046529:Microscopy, Electron, Transmission; D018384:Oxidative Stress / Q000187:drug effects*; D008517:Phytotherapy; D010938:Plant Oils / Q000008:administration & dosage / Q000627:therapeutic use*; D018517:Plant Roots / Q000737:chemistry; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D013438:Sulfhydryl Compounds / Q000819:agonists / Q000097:blood / Q000378:metabolism; D013440:Sulfides / Q000008:administration & dosage / Q000627:therapeutic use
chemical_list: D000498:Allyl Compounds; D000975:Antioxidants; D015415:Biomarkers; D007004:Hypoglycemic Agents; D000960:Hypolipidemic Agents; D010938:Plant Oils; D013438:Sulfhydryl Compounds; D013440:Sulfides; C038491:allyl sulfide
keywords: Diabetes mellitus; Garlic; Histology; Liver; Oxidative stress; Rats