title: Photoenhanced gene transfection by a star-shaped polymer consisting of a porphyrin core and poly(L-lysine) dendron arms.
abstract: A star-shaped polymer (PP-PLLD) consisting of a porphyrin (PP) core and poly(L-lysine) dendron arms (PLLD) is synthesized by the click reaction, and its ability to deliver pEGFP is investigated in this paper. It is found that PP-PLLD has a good buffer capacity and can form compact complexes with pEGFP. In vitro assay indicates that PP-PLLD shows photoenhanced gene transfection efficiency. PP-PLLD consisting of only third generation PLLD shows a higher transfected cell number than PEI under a Xe lamp at the N/P ratio of 20, and meanwhile shows a neglectable cytotoxicity to HeLa cells. Therefore, PP-PLLD with suited irradiation is a promising nontoxic and photoinducible effective gene delivery strategy, which should be encouraged in gene therapy.
authors.affiliation: Key Laboratory of Biomaterials of Guangdong Higher Education Institutes, Department of Biomedical Engineering, Jinan University, Guangzhou, 510632, China.
authors.forename: Dong
authors.initials: D
authors.lastname: Ma
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhao
authors.forename: Xiao-Yan
authors.initials: XY
authors.lastname: Zhou
authors.forename: Qian-Ming
authors.initials: QM
authors.lastname: Lin
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhang
authors.forename: Jian-Tao
authors.initials: JT
authors.lastname: Lin
authors.forename: Wei
authors.initials: W
authors.lastname: Xue
mesh_terms: D000873:Anthracenes / Q000737:chemistry*; D002021:Buffers; D016923:Cell Death; D004587:Electrophoresis, Agar Gel; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006367:HeLa Cells; D006461:Hemolysis; D006801:Humans; D008027:Light; D008422:Materials Testing; D010316:Particle Size; D010957:Plasmids / Q000378:metabolism; D011094:Polyethyleneimine / Q000737:chemistry; D011107:Polylysine / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D011166:Porphyrins / Q000737:chemistry*; D055672:Static Electricity; D017186:Titrimetry; D014162:Transfection / Q000379:methods*
chemical_list: D000873:Anthracenes; D002021:Buffers; D011108:Polymers; D011166:Porphyrins; C501244:dendron; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; D011107:Polylysine; D011094:Polyethyleneimine
keywords: photoenhanced gene transfection; poly(L-lysine) dendrimers; porphyrin; star polymers