title: Unliganded estrogen receptor alpha promotes PC12 survival during serum starvation.
abstract: Many studies have reported proliferative, differentiating or protective effects of estradiol, notably through estrogen receptor alpha (ERα). On the contrary, the ligand-independent action of ERα is currently poorly documented notably in cell protection. The stable transfection of wild type, substituted or truncated form of ERα in PC12 cells (ERα negative cell line) lead the specific study of its ligand-independent action. Hence, we demonstrate here that, in the absence of E2, the expression of ERα prevents cells from apoptosis induced by serum deprivation. This protection is not due to an ERE-mediated transcription and does not require either AF-1 or AF-2 transactivation functions. It is afforded to the Y537 residue of ERα and activation of c-Src/Stat3 signaling pathway.
authors.affiliation: Transcription, Environment and Cancer Group, Institut de Recherche sur la Santé, Environnement et Travail (IRSET), Institut National de la Santé et de la Recherche Médicale (INSERM) U1085, Université de Rennes 1, Rennes, France. francois.ferriere@univ-rennes1.fr
authors.forename: François
authors.initials: F
authors.lastname: Ferriere
authors.forename: Denis
authors.initials: D
authors.lastname: Habauzit
authors.forename: Farzad
authors.initials: F
authors.lastname: Pakdel
authors.forename: Christian
authors.initials: C
authors.lastname: Saligaut
authors.forename: Gilles
authors.initials: G
authors.lastname: Flouriot
mesh_terms: D000818:Animals; D017209:Apoptosis; D002470:Cell Survival; D047628:Estrogen Receptor alpha / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D008024:Ligands; D061986:MCF-7 Cells; D016716:PC12 Cells; D016392:Proto-Oncogene Proteins pp60(c-src) / Q000378:metabolism; D051381:Rats; D050796:STAT3 Transcription Factor / Q000378:metabolism; D044967:Serum / Q000378:metabolism*; D015398:Signal Transduction; D014443:Tyrosine / Q000378:metabolism
chemical_list: D047628:Estrogen Receptor alpha; D008024:Ligands; D050796:STAT3 Transcription Factor; D014443:Tyrosine; D016392:Proto-Oncogene Proteins pp60(c-src)