title: Small-angle X-ray solution scattering study of the multi-aminoacyl-tRNA synthetase complex reveals an elongated and multi-armed particle.
abstract: In animal cells, nine aminoacyl-tRNA synthetases are associated with the three auxiliary proteins p18, p38, and p43 to form a stable and conserved large multi-aminoacyl-tRNA synthetase complex (MARS), whose molecular mass has been proposed to be between 1.0 and 1.5 MDa. The complex acts as a molecular hub for coordinating protein synthesis and diverse regulatory signal pathways. Electron microscopy studies defined its low resolution molecular envelope as an overall rather compact, asymmetric triangular shape. Here, we have analyzed the composition and homogeneity of the native mammalian MARS isolated from rabbit liver and characterized its overall internal structure, size, and shape at low resolution by hydrodynamic methods and small-angle x-ray scattering in solution. Our data reveal that the MARS exhibits a much more elongated and multi-armed shape than expected from previous reports. The hydrodynamic and structural features of the MARS are large compared with other supramolecular assemblies involved in translation, including ribosome. The large dimensions and non-compact structural organization of MARS favor a large protein surface accessibility for all its components. This may be essential to allow structural rearrangements between the catalytic and cis-acting tRNA binding domains of the synthetases required for binding the bulky tRNA substrates. This non-compact architecture may also contribute to the spatiotemporal controlled release of some of its components, which participate in non-canonical functions after dissociation from the complex.
authors.affiliation: Laboratoire d'Enzymologie et Biochimie Structurales, Centre de Recherche de Gif, CNRS, 1 Avenue de la Terrasse, 91190 Gif-sur-Yvette, France.
authors.forename: José
authors.initials: J
authors.lastname: Dias
authors.forename: Louis
authors.initials: L
authors.lastname: Renault
authors.forename: Javier
authors.initials: J
authors.lastname: Pérez
authors.forename: Marc
authors.initials: M
authors.lastname: Mirande
mesh_terms: D000604:Amino Acyl-tRNA Synthetases / Q000737:chemistry* / Q000302:isolation & purification / Q000648:ultrastructure; D000818:Animals; D004058:Diffusion; D057446:Hydrodynamics; D008958:Models, Molecular; D010455:Peptides / Q000737:chemistry; D021122:Protein Subunits / Q000737:chemistry / Q000302:isolation & purification; D011817:Rabbits; D015203:Reproducibility of Results; D053838:Scattering, Small Angle; D012996:Solutions; D014461:Ultracentrifugation; D014961:X-Ray Diffraction
chemical_list: D010455:Peptides; D021122:Protein Subunits; D012996:Solutions; D000604:Amino Acyl-tRNA Synthetases
keywords: Aminoacyl tRNA Synthetase; Protein Complexes; Protein Structure; Protein Synthesis; X-ray Scattering