title: Scavenger receptor B1 is a potential biomarker of human nasopharyngeal carcinoma and its growth is inhibited by HDL-mimetic nanoparticles.
abstract: Nasopharyngeal carcinoma (NPC) is a very regional malignant head and neck cancer that has attracted widespread attention for its unique etiology, epidemiology and therapeutic options. To achieve high cure rates in NPC patients, theranostic approaches are actively being pursued and improved efforts remain desirable in identifying novel biomarkers and establishing effective therapeutic approaches with low long-term toxicities. Here, we discovered that the scavenger receptor class B type I (SR-B1) was overexpressed in all investigated NPC cell lines and 75% of NPC biopsies, demonstrating that SR-B1 is a potential biomarker of NPC. Additional functional analysis showed that SR-B1 has great effect on cell motility while showing no significant impact on cell proliferation. As high-density lipoproteins (HDL) exhibit strong binding affinities to SR-B1 and HDL mimetic peptides are reportedly capable of inhibiting tumor growth, we further examined the SR-B1 targeting ability of a highly biocompatible HDL-mimicking peptide-phospholipid scaffold (HPPS) nanocarrier and investigated its therapeutic effect on NPC. Results show that NPC cells with higher SR-B1 expression have superior ability in taking up the core constituents of HPPS. Moreover, HPPS inhibited the motility and colony formation of 5-8F cells, and significantly suppressed the NPC cell growth in nude mice without inducing tumor cell necrosis or apoptosis. These results indicate that HPPS is not only a NPC-targeting nanocarrier but also an effective anti-NPC drug. Together, the identification of SR-B1 as a potential biomarker and the use of HPPS as an effective anti-NPC agent may shed new light on the diagnosis and therapeutics of NPC.
authors.affiliation: Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and Technology, Wuhan 430074, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zheng
authors.forename: Yanyan
authors.initials: Y
authors.lastname: Liu
authors.forename: Honglin
authors.initials: H
authors.lastname: Jin
authors.forename: Shaotao
authors.initials: S
authors.lastname: Pan
authors.forename: Yuan
authors.initials: Y
authors.lastname: Qian
authors.forename: Chuan
authors.initials: C
authors.lastname: Huang
authors.forename: Yixin
authors.initials: Y
authors.lastname: Zeng
authors.forename: Qingming
authors.initials: Q
authors.lastname: Luo
authors.forename: Musheng
authors.initials: M
authors.lastname: Zeng
authors.forename: Zhihong
authors.initials: Z
authors.lastname: Zhang
mesh_terms: D000818:Animals; D014408:Biomarkers, Tumor / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D002277:Carcinoma; D049109:Cell Proliferation; D005260:Female; D006801:Humans; D008075:Lipoproteins, HDL / Q000008:administration & dosage / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D053758:Nanoparticles / Q000737:chemistry; D000077274:Nasopharyngeal Carcinoma; D009303:Nasopharyngeal Neoplasms / Q000188:drug therapy / Q000235:genetics / Q000378:metabolism* / Q000503:physiopathology; D010455:Peptides / Q000008:administration & dosage / Q000235:genetics / Q000378:metabolism; D051122:Scavenger Receptors, Class B / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism*
chemical_list: D014408:Biomarkers, Tumor; D008075:Lipoproteins, HDL; D010455:Peptides; C495124:SCARB1 protein, human; D051122:Scavenger Receptors, Class B
keywords: Cancer theranostics; SR-B1; biomarker; lipoprotein nanocarrier.; nasopharyngeal carcinoma