title: The immune-adjuvant effect of Japanese flounder Paralichthys olivaceus IL-1β.
abstract: IL-1β is known as a pro-inflammatory cytokine and plays a pivotal role in regulating immune response. IL-1β has been shown to influence immune responses in Japanese flounder Paralichthys olivaceus. To investigate the immune responses, a plasmid construct of pcDNA3.1-driven Japanese flounder IL-1β (pcDNA3.1-JFIL-1β) was co-injected into the muscle with Bovine serum albumin (BSA), as an antigen model, or pCI-neo driven with GFP (pCI-neo-GFP) as a vaccine model compared with the antigen or vaccine model alone, respectively. The IL-1β expression in the muscle was dramatically elevated in fish injected with pcDNA3.1-JFIL-1β on a day after injection, and the induction level was significantly higher than control groups. Moreover, pcDNA3.1-JFIL-1β significantly stimulated the gene expression of IL-1β in the kidney. The pcDNA3.1-JFIL-1β enhanced the antibody titer against BSA at 30 days after injection. In the DNA vaccine model, the antibody titer against GFP was also higher in the fish injected with pcDNA3.1-JFIL-1β than the group that injected pCI-neo-GFP alone. These results suggest that the pcDNA-driven Japanese flounder IL-1β could have potential immunoadjuvant effects.
authors.affiliation: Laboratory of Genome Science, Graduate School of Tokyo University of Marine Science and Technology, Konan 4-5-7, Minato-ku, Tokyo 108-8477, Japan.
authors.forename: Apichaya
authors.initials: A
authors.lastname: Taechavasonyoo
authors.forename: Ikuo
authors.initials: I
authors.lastname: Hirono
authors.forename: Hidehiro
authors.initials: H
authors.lastname: Kondo
mesh_terms: D000276:Adjuvants, Immunologic / Q000235:genetics / Q000494:pharmacology*; D000818:Animals; D000941:Antigens / Q000276:immunology; D005432:Flounder / Q000276:immunology*; D015870:Gene Expression; D053583:Interleukin-1beta / Q000235:genetics / Q000276:immunology* / Q000494:pharmacology*; D010957:Plasmids / Q000235:genetics / Q000276:immunology; D019444:Vaccines, DNA / Q000235:genetics / Q000276:immunology / Q000494:pharmacology
chemical_list: D000276:Adjuvants, Immunologic; D000941:Antigens; D053583:Interleukin-1beta; D019444:Vaccines, DNA
keywords: Cytokine; Immunoadjuvant; Interleukin-1β; Japanese flounder