title: [Central composite design-response surface method optimize of fang-bing nasal inhalant from components of traditional Chinese medicine for sedative and sleep aiding].
abstract: To obtain the optimal preparation technology of Fang-bing nasal inhalant from components of traditional Chinese medicine by central composite design, with an apparatus containing nasal inhalant that simulated the expiration and inspiration of nose, the dissolution in vitro of different optimized inhalant samples designed through central composite design were investigated. The accumulative release of linalool, borneol, menthol was detected with GC. Response surface methodology was used to optimize the conditions of preparation technology by establishing multiple linear regression and second-order quadratic models. Then, deviation was carried out through comparing the observed and predicted values. It was showed that the coefficient of correlation of second-order quadratic model was high. The related coefficient reached 0.999 3, 0.998 0, 0.944 9, separately. The optimum conditions of preparation technology were as following: 84.39% of alcohol concentration, the weight of starch 1.45 g and the weight of carmellose sodium (CMC-Na for short) 1.22 g. The deviations between observed and predicated values showed -0.36%, 1.52%, 2.40%, separately. In this experiment, the established model can describe the good relation between factors and indexes from preparation technology of Fang-bing nasal inhalant and the outcome of prediction is well. This optimal Fang-bing nasal inhalant was used to study its in vivo effect on model rats deprived from sleep and showed sedative and sleep aiding, which will bring an instruction on inhalants of components from traditional Chinese medicine.
authors.affiliation: College of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, China.
authors.forename: Su-Yun
authors.initials: SY
authors.lastname: Li
authors.forename: Meng-Li
authors.initials: ML
authors.lastname: Jiang
authors.forename: Li-Hong
authors.initials: LH
authors.lastname: Zhang
authors.forename: Xiao-Jin
authors.initials: XJ
authors.lastname: Xiao
authors.forename: Xiao-Dong
authors.initials: XD
authors.lastname: Li
mesh_terms: D000080462:Acyclic Monoterpenes; D000280:Administration, Inhalation; D000818:Animals; D001892:Camphanes / Q000008:administration & dosage* / Q000737:chemistry / Q000494:pharmacology; D002626:Chemistry, Pharmaceutical / Q000379:methods; D002849:Chromatography, Gas / Q000379:methods; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage* / Q000737:chemistry / Q000494:pharmacology; D006993:Hypnotics and Sedatives / Q000008:administration & dosage* / Q000737:chemistry / Q000494:pharmacology; D008297:Male; D008610:Menthol / Q000008:administration & dosage* / Q000737:chemistry / Q000494:pharmacology; D039821:Monoterpenes / Q000008:administration & dosage* / Q000737:chemistry / Q000494:pharmacology; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D012890:Sleep / Q000187:drug effects; D013678:Technology, Pharmaceutical / Q000379:methods*
chemical_list: D000080462:Acyclic Monoterpenes; D001892:Camphanes; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D006993:Hypnotics and Sedatives; D039821:Monoterpenes; D008610:Menthol; C018584:linalool; C022871:isoborneol