title: [Role of HPV16/18/45 DNA testing of cervical specimens as a triage testing in cervical cancer screening].
abstract: OBJECTIVE
  To evaluate the clinical performance of careHPV16/18/45 DNA testing of cervical specimens as a triage testing for women with positive findings during the cervical cancer screening.
  
  
  METHODS
  Eligible women aged 25-65 years were enrolled from two high-risk communities in Yangcheng County,Shanxi Province.After providing written informed consent on a voluntary base,women underwent questionnaire-based interview,gynecological examination,and sample collection.Hybrid capture 2 technology(HC2),careHPV,Avantage HPV E6 test,and visual inspection with acetic acid(VIA)were conducted as the primary screening tests at the enrollment visit.Women with any positive finding were invited to receive a second VIA and colposcopy.careHPV16/18/45 was performed as a triage testing.Any visible lesion under colposcopy was directly biopsied.Women with pathology confirmed cervical intraepithelial neoplasia grade 2 and worse(CIN2+)were treated with standard procedures.
  
  
  RESULTS
  For the self-collected and doctor-collected samples,the application of careHPV16/18/45 as a triage testing decreased the colposcopy referral to 3.2% and 3.1%,respectively.Meanwhile,the sensitivity,specificity,and positive predictive value(PPV)for CIN2+were 50.0%,97.6%,and 26.7% for women with positive self-sampling careHPV results and 63.0%,97.9%,and 34.0% for women with positive doctor-sampling careHPV results.
  
  
  CONCLUSIONS
  careHPV16/18/45 is promising as a triage testing among women with positive screening findings in low-resource settings.
authors.affiliation: Department of Cancer Epidemiology, Cancer Institute & Hospital, CAMS and PUMC, Beijing,China.
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.forename: Wen
authors.initials: W
authors.lastname: Chen
authors.forename: Le-ni
authors.initials: LN
authors.lastname: Kang
authors.forename: Feng
authors.initials: F
authors.lastname: Chen
authors.forename: Dong-ping
authors.initials: DP
authors.lastname: Guo
authors.forename: Ba-yi
authors.initials: BY
authors.lastname: Li
authors.forename: You-lin
authors.initials: YL
authors.lastname: Qiao
mesh_terms: D000328:Adult; D000368:Aged; D003127:Colposcopy; D004279:DNA, Viral / Q000032:analysis*; D055088:Early Detection of Cancer / Q000379:methods; D005260:Female; D052162:Human papillomavirus 16 / Q000235:genetics / Q000302:isolation & purification*; D052161:Human papillomavirus 18 / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D008403:Mass Screening / Q000379:methods; D008875:Middle Aged; D002583:Uterine Cervical Neoplasms / Q000175:diagnosis* / Q000821:virology*; D014626:Vaginal Smears
chemical_list: D004279:DNA, Viral