title: MiR-214 regulate gastric cancer cell proliferation, migration and invasion by targeting PTEN.
abstract: BACKGROUND
  MicroRNAs are a class of small non-coding RNAs that play an important role in various human tumor initiation and progression by regulating gene expression negatively. The aim of this study was to investigate the effect of miR-214 on cell proliferation, migration and invasion, as well as the functional connection between miR-214 and PTEN in gastric cancer.
  
  
  METHODS
  miR-214 and PTEN expression was determined in gastric cancer and matched normal tissues, and human gastric cancer cell lines by quantitative real-time PCR. The roles of miR-214 in cell proliferation, migration and invasion were analyzed with anti-miR-214 transfected cells. In addition, the regulation of PTEN by miR-214 was evaluated by Western blotting and luciferase reporter assays.
  
  
  RESULTS
  miR-214 was noted to be highly overexpressed in gastric cancer tissues and cell lines using qRT-PCR. The expression level of miR-214 is significantly associated with clinical progression and poor prognosis according to the analysis of the clinicopathologic data. We also found that the miR-214 levels are inversely correlated with PTEN in tumor tissues. And PTEN expression level is also associated with metastasis and invasion of gastric cancer. In addition, knockdown of miR-214 could significantly inhibit proliferation, migration and invasion of gastric cancer cells. Moreover, we demonstrate that PTEN is regulated negatively by miR-214 through a miR-214 binding site within the 3'-UTR of PTEN at the posttranscriptional level in gastric cancer cells.
  
  
  CONCLUSIONS
  These findings indicated that miR-214 regulated the proliferation, migration and invasion by targeting PTEN post-transcriptionally in gastric cancer. It may be a novel potential therapeutic agent for gastric cancer.
authors.affiliation: Department of Hepato-Biliary-Pancreatic Surgery, Tenth Peoples' Hospital, School of Medicine, Tongji University, 301 Middle Yanchang Road, Shanghai 200072, China.
authors.forename: Ting-Song
authors.initials: TS
authors.lastname: Yang
authors.affiliation: Department of Hepato-Biliary-Pancreatic Surgery, Tenth Peoples' Hospital, School of Medicine, Tongji University, 301 Middle Yanchang Road, Shanghai 200072, China.
authors.forename: Xiao-Hu
authors.initials: XH
authors.lastname: Yang
authors.affiliation: Department of Hepato-Biliary-Pancreatic Surgery, Tenth Peoples' Hospital, School of Medicine, Tongji University, 301 Middle Yanchang Road, Shanghai 200072, China.
authors.forename: Xu-Dong
authors.initials: XD
authors.lastname: Wang
authors.affiliation: Department of Hepato-Biliary-Pancreatic Surgery, Tenth Peoples' Hospital, School of Medicine, Tongji University, 301 Middle Yanchang Road, Shanghai 200072, China.
authors.forename: Yi-Ling
authors.initials: YL
authors.lastname: Wang
authors.affiliation: Department of Hepato-Biliary-Pancreatic Surgery, Tenth Peoples' Hospital, School of Medicine, Tongji University, 301 Middle Yanchang Road, Shanghai 200072, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Zhou
authors.affiliation: Department of Hepato-Biliary-Pancreatic Surgery, Tenth Peoples' Hospital, School of Medicine, Tongji University, 301 Middle Yanchang Road, Shanghai 200072, China.
authors.forename: Zhen-Shun
authors.initials: ZS
authors.lastname: Song