title: Celerinatantimonas yamalensis sp. nov., a cold-adapted diazotrophic bacterium from a cold permafrost brine.
abstract: A facultatively anaerobic nitrogen-fixing bacterium, strain C7(T), was isolated from a permafrost cryopeg on the Yamal Peninsula, Russia. Comparative analysis of 16S rRNA gene sequences revealed that this bacterium was closely related to Celerinatantimonas diazotrophica S-G2-2(T) with a similarity of 95.5 %. Strain C7(T) differed from Celerinatantimonas diazotrophica in its ability to hydrolyse gelatin and inability to use d-mannose, melibiose, l-rhamnose, myo-inositol, lactose, lactulose, d-mannitol, trehalose, dl-lactate, glycogen or l-proline as sole carbon sources. In addition, strain C7(T) grew over a temperature range of 0-34 °C with optimum growth at 18-22 °C. The whole-cell fatty acid profile included C16 : 0, C16 : 1ω7, C18 : 1ω7, C17 cyclo and summed feature 2 [comprising C12 : 0 aldehyde and/or unknown fatty acid 10.913 (MIDI designation) and/or iso-C16 : 1/C14 : 0 3-OH]. The DNA G+C content was 44.7 mol%. Strain C7(T) is thus considered to represent a novel species, for which the name Celerinatantimonas yamalensis sp. nov. is proposed. The type strain is C7(T) ( = VKM B-2511(T) = DSM 21888(T)).
authors.affiliation: All Russian Collection of Microorganisms (VKM), Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino, Moscow Region, Russian Federation, 142290.
authors.forename: Viktoria
authors.initials: V
authors.lastname: Shcherbakova
authors.affiliation: All Russian Collection of Microorganisms (VKM), Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino, Moscow Region, Russian Federation, 142290.
authors.forename: Nataliya
authors.initials: N
authors.lastname: Chuvilskaya
authors.affiliation: Institute of Physicochemical and Biological Problems in Soil Sciences, Russian Academy of Sciences, Pushchino, Moscow Region, Russian Federation, 142290.
authors.forename: Elizaveta
authors.initials: E
authors.lastname: Rivkina
authors.affiliation: Institute of Physicochemical and Biological Problems in Soil Sciences, Russian Academy of Sciences, Pushchino, Moscow Region, Russian Federation, 142290.
authors.forename: Nikita
authors.initials: N
authors.lastname: Demidov
authors.affiliation: Vavilov Institute of General Genetics, Russian Academy of Sciences, Gubkin street 3, Moscow, Russian Federation, 119991.
authors.forename: Victoria
authors.initials: V
authors.lastname: Uchaeva
authors.affiliation: All Russian Collection of Microorganisms (VKM), Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino, Moscow Region, Russian Federation, 142290.
authors.forename: Stanislav
authors.initials: S
authors.lastname: Suetin
authors.affiliation: All Russian Collection of Microorganisms (VKM), Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino, Moscow Region, Russian Federation, 142290.
authors.forename: Nataliya
authors.initials: N
authors.lastname: Suzina
authors.affiliation: Institute of Physicochemical and Biological Problems in Soil Sciences, Russian Academy of Sciences, Pushchino, Moscow Region, Russian Federation, 142290.
authors.forename: David
authors.initials: D
authors.lastname: Gilichinsky
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D003080:Cold Temperature; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D020563:Gammaproteobacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D006017:Glycolipids / Q000737:chemistry; D008969:Molecular Sequence Data; D009586:Nitrogen Fixation; D010088:Oxidoreductases / Q000235:genetics; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012426:Russia; D012492:Salts; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D014451:Ubiquinone / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D006017:Glycolipids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D012492:Salts; C017082:brine; D014451:Ubiquinone; C030778:ubiquinone 8; D010088:Oxidoreductases; C029130:nitrogenase reductase