title: [The expression and significance of the Notch signaling pathway molecules in tongue squamous cell carcinoma].
abstract: OBJECTIVE
  To explore the expression of Notch signaling receptors Notchl, Notch3 and its ligand Jaggedl, Jagged2 in tongue squamous cell carcinoma (TSCC).
  
  
  METHODS
  mRNA and protein expression levels of tissue samples from 74 cases of tongue cancer patients and human tongue cell line Cal-27 were detected by reverse transcriptase-polymerase chain reaction (RT-PCR), immunohistochemistry and Western blot. Its relationship with cell proliferation and clinical pathology was analyzed.
  
  
  RESULTS
  mRNA and protein expression were detected in tongue cancer tissues, adjacent tissues and cell lines. Notchl and Notch3 protein expression in tongue cancer was higher than the adjacent tissues. Jaggedl and Jagged2 protein expression in tongue cancer and adjacent tissues had no difference. Notchl and Notch3 protein had correlation with tongue cancer clinical staging. Pathway protein expression had no correlation with pathological grade, age, gender. Notchl protein expression in lymph node metastasis-positive cases was higher than in lymph node metastasis-negative cases. The expression of Notch3 and Jagged2 had correlation. Jaggedl expression grade in metastasis-positive cases was higher than in negative cases.
  
  
  CONCLUSIONS
  Notch signaling molecules have active expression in TSCC and may play important roles in tongue cancer development.
authors.affiliation: Dept. of Oral and Maxillofacial Surgery, Branch Hospital fo Stomatology, The Affiliated Zhongshan Hsopita of Sun Yat-sen University, Zhongshan, China.
authors.forename: Tonghan
authors.initials: T
authors.lastname: Zhang
authors.forename: Haichao
authors.initials: H
authors.lastname: Liu
authors.forename: Yujie
authors.initials: Y
authors.lastname: Liang
authors.forename: Lizhong
authors.initials: L
authors.lastname: Liang
authors.forename: Guiqing
authors.initials: G
authors.lastname: Liao
authors.forename: Jinan
authors.initials: J
authors.lastname: Wu
authors.forename: Hongzhang
authors.initials: H
authors.lastname: Huang
mesh_terms: D015153:Blotting, Western; D002294:Carcinoma, Squamous Cell; D002460:Cell Line; D049109:Cell Proliferation; D006801:Humans; D007150:Immunohistochemistry; D008207:Lymphatic Metastasis; D009367:Neoplasm Staging; D012333:RNA, Messenger; D051880:Receptors, Notch; D014059:Tongue; D014062:Tongue Neoplasms
chemical_list: D012333:RNA, Messenger; D051880:Receptors, Notch