title: Immunization with a multisubunit vaccine considerably reduces establishment of infective larvae in a rodent model of Brugia malayi.
abstract: Although recombinant vaccines have several advantages over conventional vaccines, protection induced by single antigen vaccines is often inadequate for a multicellular helminth parasite. Therefore, immunoprophylactic efficacy of cocktail antigen vaccines comprised of several combinations of three Brugia malayi recombinant proteins BmAF-Myo, Bm-iPGM and Bm-TPP were evaluated. Myosin+TPP and iPGM+TPP provided the best protection upon B. malayi infective larval challenge with ∼70% reduction in adult worm establishment over non-vaccinated animals that was significantly higher than the protection achieved by any single antigen vaccine. Myosin+iPGM, in contrast did not provide any enhance protection over the single recombinant protein vaccines. Specific IgG, IgM level, IgG antibody subclasses levels (IgG1, IgG2a, IgG2b, IgG3), lymphocyte proliferation, reactive oxygen species level and cytokines level were also determined to elucidate the characteristics of the protective immune responses. Thus the study undertaken provided more insight into the cocktail vaccination approach to combat LF.
authors.affiliation: Divisions of Parasitology, Central Drug Research Institute CSIR, Jankipuram Extension BS10/1, Sector 10, Sitapur Road, Lucknow 226021, UP, India.
authors.forename: Nidhi
authors.initials: N
authors.lastname: Shrivastava
authors.forename: Prashant Kumar
authors.initials: PK
authors.lastname: Singh
authors.forename: Jeetendra Kumar
authors.initials: JK
authors.lastname: Nag
authors.forename: Susheela
authors.initials: S
authors.lastname: Kushwaha
authors.forename: Shailja
authors.initials: S
authors.lastname: Misra-Bhattacharya
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000276:immunology; D000947:Antigens, Helminth / Q000276:immunology; D017178:Brugia malayi / Q000276:immunology*; D016207:Cytokines / Q000096:biosynthesis; D004605:Elephantiasis, Filarial / Q000276:immunology* / Q000469:parasitology / Q000517:prevention & control; D007114:Immunization; D007074:Immunoglobulin G / Q000276:immunology; D007075:Immunoglobulin M / Q000276:immunology; D007814:Larva / Q000276:immunology; D008213:Lymphocyte Activation / Q000276:immunology; D051189:Murinae / Q000469:parasitology; D015800:Protozoan Proteins / Q000008:administration & dosage / Q000276:immunology; D016052:Protozoan Vaccines / Q000008:administration & dosage / Q000276:immunology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000276:immunology; D022223:Vaccines, Subunit / Q000008:administration & dosage / Q000276:immunology*; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000276:immunology*
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D016207:Cytokines; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D015800:Protozoan Proteins; D016052:Protozoan Vaccines; D017382:Reactive Oxygen Species; D011994:Recombinant Proteins; D022223:Vaccines, Subunit; D014614:Vaccines, Synthetic
keywords: Bm-TPP; BmAF-Myo; Expression; Recombinant protein