title: Aspartic acid functionalized water-soluble perylene diimide as "Off-On" fluorescent sensor for selective detection Cu(2+) and ATP.
abstract: Aspartic functionalized water-soluble perylene diimide, N,N'-di(2-succinic acid)-perylene-3,4,9,10-tetracarboxylic diimide (PASP) has two absorbance maximums at 527 and 498nm (ε≈1.7×10(4)Lcm(-1)mol(-1)) and two emission peaks at 547 and 587nm respectively. Emission intensities decrease with the increase of PASP concentrations in 20-100μM ranges. Spectral titrations demonstrate that each PASP can coordinate to two Cu(2+) ions in the absence of HEPES buffer. Its stability constant is estimated to be about 1.0×10(12)L(2)mol(-2) at pH 7.20 and its coordinate stoichiometry increased to 7.5 in the same pH in the presence of HEPES buffer. The emission of PASP will be completely quenched upon formation of Cu(2+) complex. The lowest "turn-off" fluorescence detection limit was calculated to be 0.3μM Cu(2+). PASP-Cu solution was used as a "turn-on" fluorescence biosensor to detect ATP. The sensitivity towards ATP is 0.3μM in 50mM HEPES buffer at pH 7.20, which is one of the most sensitive fluorescence sensors.
authors.affiliation: Department of Chemistry, College of Sciences, Shanghai University, Shanghai 200444, PR China.
authors.forename: Lina
authors.initials: L
authors.lastname: Zhong
authors.forename: Feifei
authors.initials: F
authors.lastname: Xing
authors.forename: Yueling
authors.initials: Y
authors.lastname: Bai
authors.forename: Yongmei
authors.initials: Y
authors.lastname: Zhao
authors.forename: Shourong
authors.initials: S
authors.lastname: Zhu
mesh_terms: D000042:Absorption; D000255:Adenosine Triphosphate / Q000032:analysis*; D001224:Aspartic Acid / Q000737:chemistry* / Q000378:metabolism; D015374:Biosensing Techniques; D003300:Copper / Q000032:analysis*; D005456:Fluorescent Dyes / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007094:Imides / Q000737:chemistry* / Q000378:metabolism; D010569:Perylene / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D012995:Solubility; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D014867:Water
chemical_list: D005456:Fluorescent Dyes; D007094:Imides; C521332:perylenediimide; D014867:Water; D001224:Aspartic Acid; D010569:Perylene; D003300:Copper; D000255:Adenosine Triphosphate
keywords: ATP; Cu(2+); Fluorescent sensor; On–Off; Perylene diimide