title: Design constraints for mobile, high-speed fluorescence brain imaging in awake animals.
abstract: In this paper we present a fully self-contained imaging instrument (30 mm overall length) that is capable of recording high speed and detect relatively small fluorescent signals (0.1% ΔF/F) from brain tissues potentially containing genetically-encoded sensors or dyes. This device potentially enables the study of neuronal activity in awake and mobile animals during natural behaviors without the stress and suppression of anesthesia and restraint. The device is a fully self-contained illumination system, wide field fluorescence microscope (~ 4.8 mm² FOV-25 um lateral resolution-1.8 × magnification-0.39 NA) and CMOS image sensor (32 × 32). The total weight of the system is 10 g and is capable of imaging up to 900 fps. We present voltage dye RH1692 experiments using the system to study the somatosensory cortex of mice during whisker movements using an air puff.
authors.affiliation: The John B. Pierce Laboratory, New Haven, CT 06511, USA. aosman@jbpierce.org
authors.forename: Ahmad
authors.initials: A
authors.lastname: Osman
authors.forename: Joon Hyuk
authors.initials: JH
authors.lastname: Park
authors.forename: David
authors.initials: D
authors.lastname: Dickensheets
authors.forename: Jelena
authors.initials: J
authors.lastname: Platisa
authors.forename: Eugenio
authors.initials: E
authors.lastname: Culurciello
authors.forename: Vincent A
authors.initials: VA
authors.lastname: Pieribone
mesh_terms: D000818:Animals; D001522:Behavior, Animal / Q000502:physiology; D001698:Biomedical Engineering; D004867:Equipment Design; D005456:Fluorescent Dyes; D059907:Functional Neuroimaging / Q000295:instrumentation*; D057809:HEK293 Cells; D006801:Humans; D008027:Light; D008564:Membrane Potentials; D051379:Mice; D008856:Microscopy, Fluorescence / Q000295:instrumentation*; D009068:Movement; D055096:Optical Devices; D055620:Optical Phenomena; D012666:Semiconductors; D013003:Somatosensory Cortex / Q000502:physiology; D014738:Vibrissae / Q000294:innervation; D014851:Wakefulness
chemical_list: D005456:Fluorescent Dyes