title: Carbon-11 radiolabeling of iron-oxide nanoparticles for dual-modality PET/MR imaging.
abstract: Dual-modality imaging, using Magnetic Resonance Imaging (MRI) and Positron Emission Tomography (PET) simultaneously, is a powerful tool to gain valuable information correlating structure with function in biomedicine. The advantage of this dual approach is that the strengths of one modality can balance the weaknesses of the other. However, success of this technique requires developing imaging probes suitable for both. Here, we report on the development of a nanoparticle labeling procedure via covalent bonding with carbon-11 PET isotope. Carbon-11 in the form of [(11)C]methyl iodide was used as a methylation agent to react with carboxylic acid (-COOH) and amine (-NH2) functional groups of ligands bound to the nanoparticles (NPs). The surface coating ligands present on superparamagnetic iron-oxide nanoparticles (SPIO NPs) were radiolabeled to achieve dual-modality PET/MR imaging capabilities. The proof-of-concept dual-modality PET/MR imaging using the radiolabeled SPIO NPs was demonstrated in an in vivo experiment.
authors.affiliation: Brookhaven National Laboratory, Upton, NY 11973, USA. ramesharma26@gmail.com
authors.forename: Ramesh
authors.initials: R
authors.lastname: Sharma
authors.forename: Youwen
authors.initials: Y
authors.lastname: Xu
authors.forename: Sung Won
authors.initials: SW
authors.lastname: Kim
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Schueller
authors.forename: David
authors.initials: D
authors.lastname: Alexoff
authors.forename: S David
authors.initials: SD
authors.lastname: Smith
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: David
authors.initials: D
authors.lastname: Schlyer
mesh_terms: D000818:Animals; D002250:Carbon Radioisotopes / Q000737:chemistry; D005290:Ferric Compounds / Q000737:chemistry*; D008099:Liver / Q000000981:diagnostic imaging; D008279:Magnetic Resonance Imaging; D053768:Metal Nanoparticles / Q000737:chemistry*; D051379:Mice; D050997:Nanomedicine; D049268:Positron-Emission Tomography
chemical_list: D002250:Carbon Radioisotopes; D005290:Ferric Compounds; C000499:ferric oxide