title: Screening of different species of Phoma for the synthesis of silver nanoparticles.
abstract: We have screened 18 Phoma spp. for the mycosynthesis of silver nanoparticles (AgNPs). Out of 18, 17 Phoma spp. demonstrated mycosynthesis of AgNPs, which were characterized by UV-vis spectrophotometry, Fourier transform infrared, X-ray diffraction, transmission electron microscopy (TEM), scanning electron microscopy (SEM), nanoparticle tracking and analysis system, and zeta potential measurement. SEM and TEM analysis showed the mycosynthesis of polydispersed spherical AgNPs, with the exception by P. sorghina (MTCC-2096), which revealed the fabrication of silver nanorods. The effect of pH, temperature, silver nitrate, fungal filtrate, and light intensity was studied to understand the mechanism of mycosynthesis of AgNPs by P. sorghina. The involvement of protein was found during the mycosynthesis but the process failed to follow the Michaelis-Menton kinetics of the enzyme-catalyzed reaction. A three-step hypothetical mechanism, that is, activation, nucleation, and reduction, for the mycosynthesis of silver nanorods is proposed. The present study will be useful for the stable and rapid mycosynthesis of AgNPs. The extracellular process involved in synthesis of AgNPs was found to be rapid, simple, easy, and ecofriendly.
authors.affiliation: Department of Biotechnology, Nanobiotechnology Laboratory, Sant Gadge Baba Amravati University, Amravati, Maharashtra, India.
authors.forename: Aniket
authors.initials: A
authors.lastname: Gade
authors.forename: Swapnil
authors.initials: S
authors.lastname: Gaikwad
authors.forename: Nelson
authors.initials: N
authors.lastname: Duran
authors.forename: Mahendra
authors.initials: M
authors.lastname: Rai
mesh_terms: D001203:Ascomycota / Q000378:metabolism*; D053768:Metal Nanoparticles / Q000737:chemistry*; D012834:Silver / Q000737:chemistry / Q000378:metabolism*; D013045:Species Specificity
chemical_list: D012834:Silver
keywords: FTIR; Phoma sp; TEM; silver nanoparticles