title: A microfluidic approach for protein structure determination at room temperature via on-chip anomalous diffraction.
abstract: We report a microfluidic approach for de novo protein structure determination via crystallization screening and optimization, as well as on-chip X-ray diffraction data collection. The structure of phosphonoacetate hydrolase (PhnA) has been solved to 2.11 Åvia on-chip collection of anomalous data that has an order of magnitude lower mosaicity than what is typical for traditional structure determination methods.
authors.affiliation: Department of Chemical & Biomolecular Engineering, University of Illinois at Urbana-Champaign, USA.
authors.forename: Sarah L
authors.initials: SL
authors.lastname: Perry
authors.forename: Sudipto
authors.initials: S
authors.lastname: Guha
authors.forename: Ashtamurthy S
authors.initials: AS
authors.lastname: Pawate
authors.forename: Amrit
authors.initials: A
authors.lastname: Bhaskarla
authors.forename: Vinayak
authors.initials: V
authors.lastname: Agarwal
authors.forename: Satish K
authors.initials: SK
authors.lastname: Nair
authors.forename: Paul J A
authors.initials: PJ
authors.lastname: Kenis
mesh_terms: D000469:Alkaline Phosphatase / Q000737:chemistry*; D018360:Crystallography, X-Ray / Q000295:instrumentation / Q000379:methods*; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods*; D008958:Models, Molecular; D011487:Protein Conformation; D016962:Sinorhizobium meliloti / Q000201:enzymology; D013696:Temperature
chemical_list: D000469:Alkaline Phosphatase; C097254:phosphonoacetate hydrolase