title: Nanoscopy with more than 100,000 'doughnuts'.
abstract: We show that nanoscopy based on the principle called RESOLFT (reversible saturable optical fluorescence transitions) or nonlinear structured illumination can be effectively parallelized using two incoherently superimposed orthogonal standing light waves. The intensity minima of the resulting pattern act as 'doughnuts', providing isotropic resolution in the focal plane and making pattern rotation redundant. We super-resolved living cells in 120 μm × 100 μm-sized fields of view in <1 s using 116,000 such doughnuts.
authors.affiliation: Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Göttingen, Germany.
authors.forename: Andriy
authors.identifier: http://orcid.org/000000030265019X
authors.initials: A
authors.lastname: Chmyrov
authors.forename: Jan
authors.initials: J
authors.lastname: Keller
authors.forename: Tim
authors.initials: T
authors.lastname: Grotjohann
authors.forename: Michael
authors.initials: M
authors.lastname: Ratz
authors.forename: Elisa
authors.initials: E
authors.lastname: d'Este
authors.forename: Stefan
authors.initials: S
authors.lastname: Jakobs
authors.forename: Christian
authors.initials: C
authors.lastname: Eggeling
authors.forename: Stefan W
authors.initials: SW
authors.lastname: Hell
mesh_terms: D000818:Animals; D002460:Cell Line; D005260:Female; D006801:Humans; D008297:Male; D008856:Microscopy, Fluorescence / Q000295:instrumentation / Q000379:methods*; D036103:Nanotechnology / Q000379:methods*; D009474:Neurons / Q000648:ultrastructure; D051381:Rats; D017208:Rats, Wistar