title: Shotgun proteomic analysis of the Bombyx mori anterior silk gland: An insight into the biosynthetic fiber spinning process.
abstract: The Bombyx mori anterior silk gland (ASG) is a natural fiber manipulator for the material provided by the middle and posterior silk glands. In view of the significant role of the ASG in the liquid-crystal spinning process, a shotgun proteomics approach was taken to study the relationship between the function of proteins in the silkworm ASG and the spinning mechanism. A total of 1132 proteins with 7647 unique peptides were identified in the ASG dataset including some involved in the cuticle, ion transportation, energy metabolism, and apoptosis. Two putative cuticle-specific proteins were highly and specifically expressed in the ASG; therefore, the ASG dataset could provide clues for comprehensive understanding of the natural silk spinning mechanism in the silkworm. All MS data have been deposited in the ProteomeXchange with identifier PXD000090.
authors.affiliation: The State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, China.
authors.forename: Qiying
authors.initials: Q
authors.lastname: Yi
authors.forename: Ping
authors.initials: P
authors.lastname: Zhao
authors.forename: Xin
authors.initials: X
authors.lastname: Wang
authors.forename: Yong
authors.initials: Y
authors.lastname: Zou
authors.forename: Xiaowu
authors.initials: X
authors.lastname: Zhong
authors.forename: Chen
authors.initials: C
authors.lastname: Wang
authors.forename: Zhonghuai
authors.initials: Z
authors.lastname: Xiang
authors.forename: Qing-You
authors.initials: QY
authors.lastname: Xia
mesh_terms: D000818:Animals; D012831:Bombyx / Q000378:metabolism*; D005088:Exocrine Glands / Q000737:chemistry*; D020869:Gene Expression Profiling; D019476:Insect Proteins / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D020543:Proteome / Q000032:analysis*; D040901:Proteomics / Q000379:methods*; D047011:Silk / Q000096:biosynthesis*
chemical_list: D019476:Insect Proteins; D020543:Proteome; D047011:Silk
keywords: Animal proteomics; Anterior silk gland; Cuticle proteins; Shotgun; Silk fiber; Spinning