title: Atomic force microscopy and anodic porous allumina of nucleic acid programmable protein arrays.
abstract: The methodological aspects are here presented for the NAPPA (Nucleic Acid Programmable Protein Arrays) characterization by atomic force microscopy and anodic porous alumina. Anodic Porous Alumina represents also an advanced on chip laboratory for gene expression contained in an engineered plasmid vector. The results obtained with CdK2, CDKN1A, p53 and Jun test genes expressed on NAPPA and the future developments are discussed in terms of our pertinent and recent Patents and of their possibility to overcome some limitations of present fluorescence detection in probing protein-protein interaction in both basic sciences and clinical studies.
authors.affiliation: Nanoworld Institute President, Bergamo, Biophysics Eminent Chair University of Genova, Italy. clannicolini@gmail.com
authors.forename: Claudio
authors.initials: C
authors.lastname: Nicolini
authors.forename: Tercio Bezerra
authors.initials: TB
authors.lastname: Correia
authors.forename: Enrico
authors.initials: E
authors.lastname: Stura
authors.forename: Claudio
authors.initials: C
authors.lastname: Larosa
authors.forename: Rosanna
authors.initials: R
authors.lastname: Spera
authors.forename: Eugenia
authors.initials: E
authors.lastname: Pechkova
mesh_terms: D000537:Aluminum Oxide / Q000737:chemistry*; D018076:DNA, Complementary / Q000737:chemistry*; D020869:Gene Expression Profiling / Q000295:instrumentation / Q000379:methods; D023281:Genomics / Q000379:methods; D006801:Humans; D018625:Microscopy, Atomic Force / Q000379:methods*; D036103:Nanotechnology / Q000295:instrumentation* / Q000379:methods*; D010330:Patents as Topic; D040081:Protein Array Analysis / Q000295:instrumentation* / Q000379:methods*; D011506:Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism
chemical_list: D018076:DNA, Complementary; D011506:Proteins; D000537:Aluminum Oxide