title: Degradation of MSCRAMM target macromolecules in VLU slough by Lucilia sericata chymotrypsin 1 (ISP) persists in the presence of tissue gelatinase activity.
abstract: Venous leg ulcer slough is unpleasant to the patient and difficult to manage clinically. It harbours infection, also preventing wound management materials and dressings from supporting the underlying viable tissues. In other words, slough has significant nuisance value in the tissue viability clinic. In this study, we have sought to increase our knowledge of slough by building upon a previous but limited analysis of this necrotic tissue. In particular, slough has been probed using Western blotting for the presence of proteins with the capacity to engage microbial surface components recognising adhesive matrix macromolecules. Although the samples were difficult to resolve, we detected fibrinogen, fibronectin, IgG, collagen, human serum albumin and matrix metalloproteinase-9. Furthermore, the effect of a maggot-derived debridement enzyme, chymotrypsin 1 on macromolecules in slough was confirmed across seven patient samples. The effect of chymotrypsin 1 on slough confirms our thesis that this potential debridement enzyme could be effective in removing slough along with its associated bacteria, given its observed resistance to intrinsic gelatinase activity. In summary, we believe that the data provide scientists and clinicians with further insights into the potential molecular interactions between bacteria, wound tissue and Lucilia sericata in a clinically problematic yet scientifically interesting wound ecosystem.
authors.affiliation: Immune Modulation Research Group, School of Pharmacy, Nottingham, UK.
authors.forename: David I
authors.initials: DI
authors.lastname: Pritchard
authors.affiliation: Immune Modulation Research Group, School of Pharmacy, Nottingham, UK.
authors.forename: Alan P
authors.initials: AP
authors.lastname: Brown
mesh_terms: D000818:Animals; D002918:Chymotrypsin / Q000627:therapeutic use*; D003094:Collagen / Q000378:metabolism; D003646:Debridement / Q000379:methods*; D004175:Diptera; D005353:Fibronectins / Q000378:metabolism; D018093:Gelatinases / Q000378:metabolism; D006801:Humans; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D012867:Skin / Q000382:microbiology* / Q000503:physiopathology; D014022:Tissue Survival / Q000502:physiology*; D014647:Varicose Ulcer / Q000175:diagnosis* / Q000628:therapy*; D014945:Wound Healing / Q000502:physiology*
chemical_list: D005353:Fibronectins; D003094:Collagen; D002918:Chymotrypsin; D018093:Gelatinases; D020780:Matrix Metalloproteinase 9
keywords: Bacterial adhesins; Insect serine proteinase; Matrix metalloproteinase; Venous leg ulcer