title: Specificity and regenerability of short peptide ligands supported on polymer layers for immunoglobulin G binding and detection.
abstract: We demonstrate the specificity, regenerability, and excellent storage stability of short peptide-based systems for detection of immunoglobulin G (IgG). The bioactive component consisted of acetylated-HWRGWVA (Ac-HWRGWVA), a peptide with high IgG binding affinity, which was immobilized onto copolymer matrixes of poly(2-aminoethyl methacrylate hydrochloride-co-2-hydroxyethyl methacrylate) (poly(AMA-co-HEMA)). Surface plasmon resonance (SPR) and quartz crystal microgravimetry (QCM) were utilized with other complementary techniques to systematically investigate interfacial activities, mainly IgG binding performance as a function of the graft density and degree of polymerization of the poly(AMA-co-HEMA) support layer. Results from sodium dodecyl sulfate polyacrylamide gel electrophoresis and fluorescence microscopy indicate that the bioactive system is highly specific to IgG and resistant to nonspecific interactions when tested in mixed protein solutions.
authors.affiliation: Department of Forest Biomaterials, North Carolina State University, Raleigh, North Carolina 27695, USA. yzhang58@ncsu.edu
authors.forename: Yanxia
authors.initials: Y
authors.lastname: Zhang
authors.forename: Nafisa
authors.initials: N
authors.lastname: Islam
authors.forename: Ruben G
authors.initials: RG
authors.lastname: Carbonell
authors.forename: Orlando J
authors.initials: OJ
authors.lastname: Rojas
mesh_terms: D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007074:Immunoglobulin G / Q000737:chemistry / Q000302:isolation & purification*; D008024:Ligands; D008689:Methacrylates / Q000737:chemistry; D010455:Peptides / Q000737:chemistry*; D011102:Polyhydroxyethyl Methacrylate / Q000737:chemistry; D011108:Polymers / Q000737:chemistry*; D011485:Protein Binding; D020349:Surface Plasmon Resonance
chemical_list: D007074:Immunoglobulin G; D008024:Ligands; D008689:Methacrylates; D010455:Peptides; D011108:Polymers; D011102:Polyhydroxyethyl Methacrylate; C072703:2-aminoethylmethacrylate