title: The expression of S100A4 in human pancreatic cancer is associated with invasion.
abstract: OBJECTIVE
  Pancreatic cancer is one of the most lethal malignancies; its poor prognosis is strongly associated with invasion and metastasis. Expression of S100A4 has been reported to correlate with poor prognosis in various cancers. We have investigated the role of S100A4 in pancreatic cancer tumorigenesis and its clinicopathologic significance.
  
  
  METHODS
  Protein expression of S100A4 was examined by Western blot in pancreatic cancer cell lines and a human pancreatic ductal epithelium cell line, HPDE-6. Then the expressions of S100A4, TP53, and CD133 were examined immunohistochemically in resected specimens from 83 patients with pancreatic cancer to clarify their clinicopathologic significance. Survival analyses were performed using the Kaplan-Meier method and the Mantel-Cox method.
  
  
  RESULTS
  Forty-eight (58%) of 83 patients with pancreatic cancer positively expressed S100A4, and 50 (60%) and 29 (36%) patients positively expressed TP53 and CD133, respectively. S100A4 expression was significantly correlated with perineural invasion (P = 0.029) and invasion pattern (P = 0.001). Neither TP53 nor CD133 expression showed significant correlations with any other parameters.
  
  
  CONCLUSIONS
  Our present results suggest that S100A4 plays an important role in the invasiveness, particularly with perineural invasion and invasion pattern, of pancreatic cancer. Development of new strategies targeting S100A4 or its downstream effectors is warranted.
authors.affiliation: Department of Pathology, Tohoku University School of Medicine, Aoba-ku, Sendai, Japan.
authors.forename: Nobukazu
authors.initials: N
authors.lastname: Tsukamoto
authors.forename: Shinichi
authors.initials: S
authors.lastname: Egawa
authors.forename: Masanori
authors.initials: M
authors.lastname: Akada
authors.forename: Keiko
authors.initials: K
authors.lastname: Abe
authors.forename: Yuriko
authors.initials: Y
authors.lastname: Saiki
authors.forename: Naoyuki
authors.initials: N
authors.lastname: Kaneko
authors.forename: Satoru
authors.initials: S
authors.lastname: Yokoyama
authors.forename: Kentaro
authors.initials: K
authors.lastname: Shima
authors.forename: Akihiro
authors.initials: A
authors.lastname: Yamamura
authors.forename: Fuyuhiko
authors.initials: F
authors.lastname: Motoi
authors.forename: Hisashi
authors.initials: H
authors.lastname: Abe
authors.forename: Hiroki
authors.initials: H
authors.lastname: Hayashi
authors.forename: Kazuyuki
authors.initials: K
authors.lastname: Ishida
authors.forename: Takuya
authors.initials: T
authors.lastname: Moriya
authors.forename: Takahiro
authors.initials: T
authors.lastname: Tabata
authors.forename: Emiko
authors.initials: E
authors.lastname: Kondo
authors.forename: Naomi
authors.initials: N
authors.lastname: Kanai
authors.forename: Zhaodi
authors.initials: Z
authors.lastname: Gu
authors.forename: Makoto
authors.initials: M
authors.lastname: Sunamura
authors.forename: Michiaki
authors.initials: M
authors.lastname: Unno
authors.forename: Akira
authors.initials: A
authors.lastname: Horii
mesh_terms: D000071916:AC133 Antigen; D000230:Adenocarcinoma / Q000378:metabolism* / Q000473:pathology; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015703:Antigens, CD / Q000378:metabolism; D015153:Blotting, Western; D021441:Carcinoma, Pancreatic Ductal / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D006023:Glycoproteins / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D053208:Kaplan-Meier Estimate; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D010190:Pancreatic Neoplasms / Q000378:metabolism* / Q000473:pathology; D010455:Peptides / Q000378:metabolism; D011379:Prognosis; D000071999:S100 Calcium-Binding Protein A4; D009418:S100 Proteins / Q000096:biosynthesis*; D046888:Tissue Array Analysis; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism
chemical_list: D000071916:AC133 Antigen; D015703:Antigens, CD; D006023:Glycoproteins; C000606023:PROM1 protein, human; D010455:Peptides; D000071999:S100 Calcium-Binding Protein A4; D009418:S100 Proteins; D016159:Tumor Suppressor Protein p53; C077591:S100A4 protein, human