title: Genetic diversity in exon 2 of the major histocompatibility complex class II DQB1 locus in Nigerian goats.
abstract: The DQB1 locus is located in the major histocompatibility complex (MHC) class II region and involved in immune response. We identified 20 polymorphic sites in a 228 bp fragment of exon 2, one of the most critical regions of the MHC DQB1 gene, in 60 Nigerian goats. Four sites are located in the peptide binding region, and 10 amino acid substitutions are peculiar to Nigerian goats, compared with published sequences. A significantly higher ratio of nonsynonymous/synonymous substitutions (dN/dS) suggests that allelic sequence evolution is driven by balancing selection (P < 0.01). In silico functional analysis using PANTHER predicted that substitution P56R, with a subPSEC score of -4.00629 (Pdeleterious = 0.73229), is harmful to protein function. The phylogenetic tree from consensus sequences placed the two northern breeds closer to each other than either was to the southern goats. This first report of sequence diversity at the DQB1 locus for any African goat breed may be useful in the search for disease-resistant genotypes.
authors.affiliation: Department of Animal Science, Cornell University, Ithaca, NY, 14853, USA.
authors.forename: Abdulmojeed
authors.initials: A
authors.lastname: Yakubu
authors.forename: Adebowale E
authors.initials: AE
authors.lastname: Salako
authors.forename: Marcos
authors.initials: M
authors.lastname: De Donato
authors.forename: Michael I
authors.initials: MI
authors.lastname: Takeet
authors.forename: Sunday O
authors.initials: SO
authors.lastname: Peters
authors.forename: Mufliat A
authors.initials: MA
authors.lastname: Adefenwa
authors.forename: Moses
authors.initials: M
authors.lastname: Okpeku
authors.forename: Mathew
authors.initials: M
authors.lastname: Wheto
authors.forename: Brilliant O
authors.initials: BO
authors.lastname: Agaviezor
authors.forename: Timothy M
authors.initials: TM
authors.lastname: Sanni
authors.forename: Oyeyemi O
authors.initials: OO
authors.lastname: Ajayi
authors.forename: Gbolabo O
authors.initials: GO
authors.lastname: Onasanya
authors.forename: Oludotun J
authors.initials: OJ
authors.lastname: Ekundayo
authors.forename: Babatunde M
authors.initials: BM
authors.lastname: Ilori
authors.forename: Samuel A
authors.initials: SA
authors.lastname: Amusan
authors.forename: Ikhide G
authors.initials: IG
authors.lastname: Imumorin
mesh_terms: D000818:Animals; D005091:Exons; D005802:Genes, MHC Class II; D014644:Genetic Variation; D006041:Goats / Q000235:genetics* / Q000276:immunology; D000949:Histocompatibility Antigens Class II / Q000737:chemistry; D008285:Major Histocompatibility Complex / Q000235:genetics* / Q000276:immunology; D008969:Molecular Sequence Data; D009549:Nigeria; D010802:Phylogeny; D011110:Polymorphism, Genetic; D020641:Polymorphism, Single Nucleotide; D011485:Protein Binding; D012641:Selection, Genetic; D016415:Sequence Alignment; D020539:Sequence Analysis, Protein
chemical_list: D000949:Histocompatibility Antigens Class II