title: New PCR test for detection of Candida glabrata based on the molecular target chosen by the RAPD technique.
abstract: Rapid, reliable diagnosis is a necessary condition for the successful treatment of infections. Such diagnostic assays are continually being developed. The paper presents a method for selecting the molecular target for PCR-based diagnostics based on the comparison of RAPD patterns. A sequence encoding Candida glabrata CBS138 hypothetical protein was selected. The limit of detection for PCR and real-time PCR reactions with DNA extracted from blood samples spiked with Candida glabrata was estimated at 1 CFU/ml. The application of the assays developed in this study would thus seem to be promising as a complementary method in the diagnostics of C. glabrata infections.
authors.affiliation: Department of Microbiology, Gdańsk University of Technology, Poland. annbrill@pg.gda.pl
authors.forename: Anna
authors.initials: A
authors.lastname: Olchawa
authors.forename: Beata
authors.initials: B
authors.lastname: Krawczyk
authors.forename: Anna
authors.initials: A
authors.lastname: Brillowska-Dabrowska
mesh_terms: D041221:Candida glabrata / Q000235:genetics* / Q000302:isolation & purification*; D004271:DNA, Fungal / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D019105:Random Amplified Polymorphic DNA Technique
chemical_list: D004271:DNA, Fungal