title: Radioprotective role of clinical drug diclofenac sodium.
abstract: The potential of clinical drug diclofenac sodium which is routinely used in clinics as non-steroid anti-inflammatory drugs opens a new insight in development of radioprotector. The drug has shown its potential radioprotective efficacy in clonogenic cell survival in Chinese hamster V79 cells with a DMF of 1.4. The pBR322 plasmid DNA gets damaged by radiation in which the supercoiled form gradually disappears with increasing radiation dose. Diclofenac sodium has shown its radioprotective potential by scavenging radiation induced free radicals which are depicted by its ability in restoring the fraction of supercoiled form of plasmid DNA back to normal. 250μM concentration of the drug provides GSSB yield of 3.65±0.5×10(-9)Da(-1)Gy(-1). This drug has shown to have a free radical scavenging activity because the radical cation with blue color is converted to the colorless neutral form by addition of diclofenac sodium in ABTS assay. Whole body survival study has shown it to protect 45.5% of C57BL/6 mice at a lethal irradiation dose of 9Gy. Therefore, this molecule offers a potential radioprotective ability besides being used routinely as analgesic and anti inflammatory compound.
authors.affiliation: Chemical Radioprotector & Radiation Dosimetry Research Group, Division of Radiation Bioscience, Institute of Nuclear Medicine and Allied Sciences, Delhi, India.
authors.forename: Amit
authors.initials: A
authors.lastname: Alok
authors.forename: J S
authors.initials: JS
authors.lastname: Adhikari
authors.forename: N K
authors.initials: NK
authors.lastname: Chaudhury
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D002460:Cell Line / Q000187:drug effects / Q000528:radiation effects; D003037:Cobalt Radioisotopes; D003114:Colony-Forming Units Assay; D006224:Cricetinae; D003412:Cricetulus; D004008:Diclofenac / Q000494:pharmacology*; D004307:Dose-Response Relationship, Radiation; D004353:Drug Evaluation, Preclinical; D005347:Fibroblasts / Q000187:drug effects* / Q000528:radiation effects; D016166:Free Radical Scavengers / Q000494:pharmacology*; D005720:Gamma Rays; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D015394:Molecular Structure; D010957:Plasmids / Q000187:drug effects / Q000528:radiation effects; D011833:Radiation Injuries, Experimental / Q000209:etiology / Q000517:prevention & control; D011836:Radiation Tolerance / Q000187:drug effects; D011837:Radiation-Protective Agents / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000032:analysis; D014916:Whole-Body Irradiation / Q000009:adverse effects
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D003037:Cobalt Radioisotopes; D016166:Free Radical Scavengers; D011837:Radiation-Protective Agents; D017382:Reactive Oxygen Species; D004008:Diclofenac
keywords: Diclofenac sodium; Dose modification factor (DMF); Radical scavenging; Radioprotection; Reactive oxygen species (ROS)