title: [Affinity capture of specific DNA fragments with the use of short synthetic sequences].
abstract: The ability of short peptide nucleic acid (PNA) oligomers and oligonucleotides containing modified residues of 5-methylcitidine, 2-aminoadenosine and 5-propynyl-2'-deoxyuridine (strong binding oligonucleotides, SBO) to affinity capture the target double-stranded DNA fragment from mixture by means of the end invasion was compared. Both types of probes were highly effective at the conditions used. The SBO-based probes may represent a handy and easily prepared alternative to PNA for selection of target DNA fragments from mixtures.
authors.forename: V S
authors.initials: VS
authors.lastname: Mikhaĭlov
authors.forename: V K
authors.initials: VK
authors.lastname: Potapov
authors.forename: R N
authors.initials: RN
authors.lastname: Amirkhanov
authors.forename: N V
authors.initials: NV
authors.lastname: Amirkhanov
authors.forename: S S
authors.initials: SS
authors.lastname: Bulanenkova
authors.forename: S B
authors.initials: SB
authors.lastname: Akopov
authors.forename: V F
authors.initials: VF
authors.lastname: Zarytova
authors.forename: L G
authors.initials: LG
authors.lastname: Nikolaev
authors.forename: E D
authors.initials: ED
authors.lastname: Sverdlov
mesh_terms: D000241:Adenosine / Q000031:analogs & derivatives / Q000737:chemistry; D001483:Base Sequence; D004247:DNA / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D009841:Oligonucleotides / Q000138:chemical synthesis* / Q000737:chemistry; D020135:Peptide Nucleic Acids / Q000138:chemical synthesis* / Q000737:chemistry / Q000235:genetics; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D012097:Repressor Proteins / Q000737:chemistry
chemical_list: D009841:Oligonucleotides; D020135:Peptide Nucleic Acids; D012097:Repressor Proteins; C498646:ZBTB32 protein, human; C033854:2-aminoadenosine; D004247:DNA; D000241:Adenosine