title: A new thiophenyl pyrazoline fluorescent probe for Cu2+ in aqueous solution and imaging in live cell.
abstract: A new thiophenyl pyrazoline probe for Cu(2+) in aqueous solution was synthesized and characterized by IR, NMR, HRMS and X-ray analysis. The probe displays remarkably high selectivity and sensitivity for Cu(2+) with a detection limit of 1.919 × 10(-7) M in aqueous solution (EtOH:HEPES = 1:1, v/v, 0.02 M, pH = 7.2). In addition, the probe is further successfully used to image Cu(2+) in living cells and the probe possesses good reversibility.
authors.affiliation: School of Chemistry and Chemical Engineering, Shandong University, Jinan, 250100, People's Republic of China.
authors.forename: Meng-Meng
authors.initials: MM
authors.lastname: Li
authors.forename: Wen-Bo
authors.initials: WB
authors.lastname: Zhao
authors.forename: Ting-Ting
authors.initials: TT
authors.lastname: Zhang
authors.forename: Wei-Liu
authors.initials: WL
authors.lastname: Fan
authors.forename: Yu
authors.initials: Y
authors.lastname: Xu
authors.forename: Yu
authors.initials: Y
authors.lastname: Xiao
authors.forename: Jun-Ying
authors.initials: JY
authors.lastname: Miao
authors.forename: Bao-Xiang
authors.initials: BX
authors.lastname: Zhao
mesh_terms: D003300:Copper / Q000032:analysis*; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D008958:Models, Molecular; D015394:Molecular Structure; D011720:Pyrazoles / Q000138:chemical synthesis / Q000737:chemistry*; D012996:Solutions; D014407:Tumor Cells, Cultured; D014867:Water / Q000737:chemistry
chemical_list: D005456:Fluorescent Dyes; D011720:Pyrazoles; D012996:Solutions; D014867:Water; D003300:Copper