title: A comparative evaluation of cone beam CT and micro-CT on trabecular bone structures in the human mandible.
abstract: OBJECTIVE
  The main purpose of this study was to determine the accuracy of cone beam CT (CBCT) in measuring the trabecular bone microstructure, in comparison with micro-CT. The subobjective was to examine to what extent bone quality assessment is influenced by X-ray tube current and voltage settings as well as soft tissue surrounding the bone.
  
  
  METHODS
  Eight human mandibular bone samples were scanned using three different clinical exposure protocol within water (W1-3) and without water (NW1-3) by a high-resolution (80 µm) CBCT machine (3D Accuitomo 170(®); Morita, Kyoto, Japan). Subsequently, the samples underwent micro-CT scanning (SkyScan 1174®; SkyScan, Antwerp, Belgium). After image acquisition, similar volumes of interest of the trabecular structures captured with CBCT and micro-CT were aligned with each other. Segmentation was then performed, and the morphometric parameters were quantified within the volumes of interest by CTAn software (CTAnalyser(®); SkyScan, Antwerp, Belgium). Descriptive statistical analyses and multiple comparisons between all protocols were applied in R software.
  
  
  RESULTS
  High positive Pearson's correlation coefficients were observed between CBCT and micro-CT protocols for all tested morphometric indices except for trabecular thickness. No significant differences were observed between all exposure protocols except for trabecular separation. When examining the soft-tissue effect on trabecular bone structures, no significant differences between NW (1-3) and W (1-3) protocols were observed for all variables.
  
  
  CONCLUSIONS
  The present study demonstrated the potential of high-resolution CBCT imaging for in vivo applications of quantitative bone morphometry and bone quality assessment. However, the overestimation of morphometric parameters and acquisition settings in CBCT must be taken into account.
authors.affiliation: Department of Oral Health Science, Oral Imaging Center, KU Leuven, Leuven, Belgium.
authors.forename: J
authors.initials: J
authors.lastname: Van Dessel
authors.forename: Y
authors.initials: Y
authors.lastname: Huang
authors.forename: M
authors.initials: M
authors.lastname: Depypere
authors.forename: I
authors.initials: I
authors.lastname: Rubira-Bullen
authors.forename: F
authors.initials: F
authors.lastname: Maes
authors.forename: R
authors.initials: R
authors.lastname: Jacobs
mesh_terms: D015519:Bone Density / Q000502:physiology; D054893:Cone-Beam Computed Tomography / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods; D021621:Imaging, Three-Dimensional / Q000379:methods; D007575:Jaw, Edentulous / Q000000981:diagnostic imaging; D008334:Mandible / Q000000981:diagnostic imaging*; D010519:Periodontium / Q000000981:diagnostic imaging; D011829:Radiation Dosage; D012984:Software; D055114:X-Ray Microtomography / Q000379:methods*
keywords: bone architecture; cone beam CT; micro-computed tomography; quantitative bone morphometry