title: Determination of labile Fe(II) species complexed with seawater extractable organic matter under seawater conditions based on the kinetics of ligand-exchange reactions with ferrozine.
abstract: A fertilizer, comprised of a mixture of steel slag and compost, was used to restore seaweed beds in barren coastal areas. Complex Fe(II) species, supplied by steel slag, play a significant role in supplying Fe(II) to coastal areas and stimulating seaweed growth. Seawater extractable organic matter (SWEOM) from compost is generally assumed to serve as a chelator of Fe(II) in the fertilizer. It is considered that the bioavailability of Fe(II)-SWEOM complexes is higher in the dissociable (labile) species. In the present study, a method for determining labile species of Fe(II)-SWEOM complexes in seawater (pH 8.0, I = 0.7) was developed. The method is based on a ligand-exchange reaction between SWEOM and ferrozine (FZ). Because Fe(II) is readily oxidized to Fe(III) under normal seawater conditions, ascorbic acid was added as an antioxidant. The coloring for the Fe-FZ complex in the presence of SWEOM was retarded. This retarding can be attributed to a ligand-exchange reaction between FZ and labile Fe(II)-SWEOM complexes. Conditional binding constants for the labile Fe(II)-SWEOM complexes and binding capacities of labile sites in SWEOM to Fe(II) were evaluated for a variety of total Fe(II) concentrations.
authors.affiliation: Laboratory of Chemical Resource, Division of Sustainable Resources Engineering, Graduate School of Engineering, Hokkaido University, Sapporo, Japan.
authors.forename: Hisanori
authors.initials: H
authors.lastname: Iwai
authors.forename: Masami
authors.initials: M
authors.lastname: Fukushima
authors.forename: Mitsuo
authors.initials: M
authors.lastname: Yamamoto