title: A protein-based electrochemical biosensor array platform for integrated microsystems.
abstract: This paper elucidates challenges in integrating different classes of proteins into a microsystem and presents an electrochemical array strategy for heterogeneous protein-based biosensors. The overlapping requirements and limitations imposed by biointerface formation, electrochemical characterization, and microsystem fabrication are identified. A planar electrode array is presented that synergistically resolves these requirements using thin film Au and Ag/AgCl electrodes on a dielectric substrate. Using molecular self-assembly, electrodes were modified by nano-structures of two diverse proteins, alkali ion-channel protein and alcohol dehydrogenase enzyme. Electrochemical impedance spectroscopy and cyclic voltammetry measurements were performed to characterize sensor response to alkali ion and alcohol, respectively. This work demonstrates the viability of the electrochemical microsystem platform for heterogeneous protein-based biosensor interfaces.
authors.affiliation: Electrical and Computer Engineering Department, Michigan State University, East Lansing, MI 48824, USA. abrahuang@gmail.com
authors.lastname: Yue Huang
authors.lastname: Ying Liu
authors.forename: B L
authors.initials: BL
authors.lastname: Hassler
authors.forename: R M
authors.initials: RM
authors.lastname: Worden
authors.forename: A J
authors.initials: AJ
authors.lastname: Mason
mesh_terms: D000426:Alcohol Dehydrogenase / Q000737:chemistry*; D001426:Bacterial Proteins / Q000737:chemistry*; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods; D055664:Electrochemical Techniques / Q000295:instrumentation / Q000379:methods; D004800:Enzymes, Immobilized / Q000737:chemistry; D006046:Gold / Q000737:chemistry; D008839:Microelectrodes; D012834:Silver / Q000737:chemistry; D018030:Silver Compounds / Q000737:chemistry; D046968:Thermoanaerobacter / Q000201:enzymology*
chemical_list: D001426:Bacterial Proteins; D004800:Enzymes, Immobilized; D018030:Silver Compounds; D012834:Silver; D006046:Gold; D000426:Alcohol Dehydrogenase; C037548:silver chloride