title: Mycobacterium tuberculosis PPE protein Rv0256c induces strong B cell response in tuberculosis patients.
abstract: Tuberculosis (TB) is one of the most important diseases of humans and major public health problem worldwide. Early and accurate diagnosis of TB is necessary for the treatment, prevention, and control of TB. Therefore, it is important to identify suitable antigens that can differentiate active tuberculosis patients from BCG-vaccinated individuals. In the present study, we have used Rv0256c (PPE2) protein of Mycobacterium tuberculosis to screen the sera of infected patients belonging to different clinical TB presentations, and BCG-vaccinated clinically healthy individuals by enzyme immunoassay. Our results demonstrated that Rv0256c displayed stronger and specific immunoreactivity against the sera obtained from clinically active tuberculosis patients compared to PPD and ESAT-6 and could differentiate the TB-patients from the BCG-vaccinated controls. Importantly, Rv0256c was also found to detect even the extrapulmonary and smear-negative pulmonary cases which often are tedious and difficult to detect using conventional diagnostic methods. This study suggests that Rv0256c can be used as a potential marker for the serodiagnosis of tuberculosis patients.
authors.affiliation: Laboratory of Molecular Cell Biology, Centre for DNA Fingerprinting and Diagnostics (CDFD), Gruhakalpa Building, Nampally, Hyderabad 500001, India.
authors.forename: Philip Raj
authors.initials: PR
authors.lastname: Abraham
authors.affiliation: Bhagwan Mahavir Medical Research Center, 10-1-1, Mahavir Marg, A.C. Guards, Hyderabad 500004, India.
authors.forename: Gaddam Suman
authors.initials: GS
authors.lastname: Latha
authors.affiliation: Bhagwan Mahavir Medical Research Center, 10-1-1, Mahavir Marg, A.C. Guards, Hyderabad 500004, India; Division of Immunology and Molecular Biology, LEPRA Society-Blue Peter Research Center, Hyderabad 501301, India.
authors.forename: Vijaya Lakshmi
authors.initials: VL
authors.lastname: Valluri
authors.affiliation: Laboratory of Molecular Cell Biology, Centre for DNA Fingerprinting and Diagnostics (CDFD), Gruhakalpa Building, Nampally, Hyderabad 500001, India. Electronic address: sangita@cdfd.org.in.
authors.forename: Sangita
authors.initials: S
authors.lastname: Mukhopadhyay
mesh_terms: D000328:Adult; D000907:Antibodies, Bacterial / Q000276:immunology / Q000378:metabolism*; D000942:Antigens, Bacterial / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology; D001426:Bacterial Proteins / Q000276:immunology; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male; D008875:Middle Aged; D009169:Mycobacterium tuberculosis / Q000276:immunology; D012698:Serologic Tests; D014376:Tuberculosis / Q000175:diagnosis*; D055815:Young Adult
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; C582018:PPE2 protein, Mycobacterium tuberculosis
keywords: Enzyme immunoassay; Mycobacterium tuberculosis; PPE protein Rv0256c; Serodiagnosis