title: Differences in the roles of a glutamine amidotransferase subunit of pyridoxal 5'-phosphate synthase between Bacillus circulans and Bacillus subtilis.
abstract: BtrC2 of the butirosin producer Bacillus circulans is a non-catalytic subunit of 2-deoxy-scyllo-inosose (DOI) synthase that is involved in butirosin biosynthesis, and also a homolog of glutamine amidotransferase subunit (PdxT) of pyridoxal 5'-phosphate (PLP) synthase of Bacillus subtilis. BtrC2 has been found to have functions in B. circulans both in primary and secondary metabolism. In this study, we investigated the properties of PdxT of B. subtilis in order to determine whether the property of enzyme stabilization is universal among PdxT homologs. Complementation with PdxT in the btrC2 disruptant of B. circulans restored the growth and short-term production of antibiotics, but long-term production of antibiotics cannot be restored. Additionally, PdxT did not bind physically with or stabilize BtrC. Our results indicate that the function of BtrC2 in secondary metabolism is specific properties, not universal among PdxT homologs.
authors.affiliation: Department of Correlative Study of Physics and Chemistry, Graduate School of Integrated Basic Sciences, Nihon University, Tokyo 156-8550, Japan.
authors.forename: Shiori
authors.initials: S
authors.lastname: Itagaki
authors.forename: Minami
authors.initials: M
authors.lastname: Haga
authors.forename: Yuji
authors.initials: Y
authors.lastname: Oikawa
authors.forename: Ayaka
authors.initials: A
authors.lastname: Sakoda
authors.forename: Yoshie
authors.initials: Y
authors.lastname: Ohke
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Sawada
authors.forename: Tadashi
authors.initials: T
authors.lastname: Eguchi
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Tamegai
mesh_terms: D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000096:biosynthesis; D001412:Bacillus subtilis / Q000201:enzymology* / Q000378:metabolism; D008025:Ligases / Q000737:chemistry*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D011487:Protein Conformation; D021122:Protein Subunits / Q000737:chemistry* / Q000378:metabolism*; D013045:Species Specificity; D000637:Transaminases / Q000737:chemistry / Q000378:metabolism*
chemical_list: D000900:Anti-Bacterial Agents; D021122:Protein Subunits; D000637:Transaminases; C020656:glutamine-pyruvate aminotransferase; D008025:Ligases