title: The structurally effect of surface coated rhamnogalacturonan I on response of the osteoblast-like cell line SaOS-2.
abstract: Osseointegration is important when implants are inserted into the bone and can be improved by biochemical surface coating of the implant. In this paper enzymatically modified rhamnogalacturonan I (RG-I) from apple and lupin was used for biochemical coating of aminated surfaces and the importance of the quality of RG-I, the nature of the binding, the fine structure of RG-I, and its effect on SaOS-2 cell line cultured on coated surfaces was investigated. SaOS-2 cells are osteoblast-like cells and a well-established in vitro model of bone-matrix forming osteoblasts. Purification by gel filtration could remove small fragments of galacturonic acid (GalA) and binding studies showed that the purity of the RG-I molecules was important for the quality of the coating. The structure of RG-I and osteoblast-like cells' viability were positively correlated so that high content of 1,4-linked galactose (Gal) and a low content of arabinose in the RG-I molecules favored cell viability. These results indicate that coating of implants with RG-I affect osseointegration positively.
authors.affiliation: Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, Thorvaldsensvej 40, Frederiksberg C 1871, Denmark; Copenhagen Center for Glycomics, Institute for Cellular and Molecular Medicine, Faculty of Health and Medicine Sciences, University of Copenhagen, Blegdamsvej 3 B, Copenhagen N 2200, Denmark.
authors.forename: Rikke
authors.initials: R
authors.lastname: Svava
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Gurzawska
authors.forename: Yu
authors.initials: Y
authors.lastname: Yihau
authors.forename: Kenneth Brian
authors.initials: KB
authors.lastname: Haugshøj
authors.forename: Kai
authors.initials: K
authors.lastname: Dirscherl
authors.forename: Steven B
authors.initials: SB
authors.lastname: Levery
authors.forename: Niklas Rye
authors.initials: NR
authors.lastname: Jørgensen
authors.forename: Klaus
authors.initials: K
authors.lastname: Gotfredsen
authors.forename: Iben
authors.initials: I
authors.lastname: Damager
authors.forename: Peter
authors.initials: P
authors.lastname: Ulvskov
authors.forename: Bodil
authors.initials: B
authors.lastname: Jørgensen
mesh_terms: D002460:Cell Line; D002470:Cell Survival; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D015921:Dental Implants; D006801:Humans; D031297:Lupinus / Q000737:chemistry; D027845:Malus / Q000737:chemistry; D010006:Osteoblasts / Q000166:cytology*; D010368:Pectins / Q000737:chemistry*
chemical_list: D020099:Coated Materials, Biocompatible; D015921:Dental Implants; C042491:rhamnogalacturonan I; D010368:Pectins
keywords: Osseointegration; SaOS-2 cell line; dental implant; polysaccharide; surface modification