title: [Study on intestinal absorption of tilianin in rats of single-pass perfusion model].
abstract: OBJECTIVE
  To study the intestinal absorption mechanism of tilianin in rats.
  
  
  METHODS
  The single-pass perfusion model was established in rats. The concentrations of tilianin with in situ intestinal perfusion were determined by HPLC. The impact factors, such as verapamil, reserpine, phloridzin and rifampicin, on Ka and Papp of tilianin in rat jejunum were investigated.
  
  
  RESULTS
  Compared with the control group, Ka and Papp in rat jejunum were significantly higher after being added with verapamil and reserpine (P < 0.05). Papp of tilianin in rat jejunum was significantly lower after being added with phloridzin (P < 0.05). Compared with the control group, both Ka and Papp of tilianin in rat jejunum were not significantly higher after being added with rifampicin.
  
  
  CONCLUSIONS
  Tilianin is the substrate of P-gp, BCRP and SGLT1. The effluent effect of P-gp and BCRP is the main mechanism of tilianin in intestinal absorption, indicating that tilianin can realize intestinal absorption and transport by relying on SGLT1. Tilianin is not the substrate of bile salt transporter protein.
authors.affiliation: Pharmacy of College, Shihezi University, Shihezi 832002, China.
authors.forename: Ting-Ting
authors.initials: TT
authors.lastname: Wang
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.forename: Yong
authors.initials: Y
authors.lastname: Yuan
authors.forename: Li-Ping
authors.initials: LP
authors.lastname: Wang
authors.forename: Xin-Chun
authors.initials: XC
authors.lastname: Wang
mesh_terms: D000818:Animals; D018938:Caco-2 Cells; D004365:Drugs, Chinese Herbal / Q000493:pharmacokinetics*; D005260:Female; D005419:Flavonoids / Q000493:pharmacokinetics*; D006027:Glycosides / Q000493:pharmacokinetics*; D006801:Humans; D007408:Intestinal Absorption; D019686:Lamiaceae / Q000737:chemistry*; D008297:Male; D008954:Models, Biological; D010477:Perfusion; D051381:Rats; D017208:Rats, Wistar
chemical_list: D004365:Drugs, Chinese Herbal; D005419:Flavonoids; D006027:Glycosides; C426884:tilianin