title: A fluorogenic assay for screening Sirt6 modulators.
abstract: A fluorogenic high-throughput assay suitable for screening Sirt6 modulators is developed based on the recently discovered efficient activity of Sirt6 to hydrolyze myristoyl lysine. Sirt6 modulators will be useful in investigating the function of Sirt6 and protein lysine fatty acylation.
authors.affiliation: Department of Chemistry and Chemical Biology, Baker Laboratory, Cornell University, Ithaca, NY 14853, USA.
authors.forename: Jing
authors.initials: J
authors.lastname: Hu
authors.forename: Bin
authors.initials: B
authors.lastname: He
authors.forename: Shiva
authors.initials: S
authors.lastname: Bhargava
authors.forename: Hening
authors.initials: H
authors.lastname: Lin
mesh_terms: D057075:Enzyme Assays / Q000379:methods*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D057166:High-Throughput Screening Assays / Q000379:methods; D006801:Humans; D006868:Hydrolysis; D008239:Lysine / Q000031:analogs & derivatives* / Q000378:metabolism; D010455:Peptides / Q000737:chemistry / Q000378:metabolism; D037761:Sirtuins / Q000037:antagonists & inhibitors / Q000378:metabolism*; D013050:Spectrometry, Fluorescence / Q000379:methods*
chemical_list: D004791:Enzyme Inhibitors; D010455:Peptides; C516592:SIRT6 protein, human; D037761:Sirtuins; D008239:Lysine