title: Characterization of extended-spectrum β-lactamase (ESBL) producing Escherichia coli strains isolated from animal and human clinical samples in Hungary in 2006-2007.
abstract: The proportion of Escherichia coli non-susceptible to 3(rd) generation cephalosprins from invasive clinical samples has risen in Hungary from 5.1 per cent in 2006 to 15.5 per cent in 2011. The prevalence of ESBL-production in E. coli of animal origin remains unknown. During the first stage of a probe forty-five human and 18 animal ESBL-producing E. coli strains isolated in 2006-2007 were investigated. The human strains were representatively selected from a collection of 113 ESBL-producing isolates sent to the national reference center from local laboratories across the country. A variety of ESBLs were detected (SHV-2, -5, -12, CTX-M-32) with CTX-M-15 being the most common in human and CTX-M-1 the dominant in animal isolates. Genetic characterization revealed that thirty-six human isolates (80 per cent) belonged to either the phylogenetic group (PG) B2 or D. Conversely, 15 animal isolates (83 per cent) proved to be members of the A and B1 commensal PGs. Furthermore 46 per cent of human isolates (21/45) from 12 centres belonged to the international O25-ST131/B2 clone while nine isolates from seven centers showed the O15 serotype. Pulsed-field gel electrophoresis (PFGE) detected 22 and 11 diverse pulsotypes among 45 human and 18 animal isolates, respectively. The human and animal strains did not share any pulsotypes.
authors.affiliation: National Center for Epidemiology, Gyáli út 2-6 H-1097, Budapest, Hungary. toth.akos@oek.antsz.hu
authors.forename: Akos
authors.initials: A
authors.lastname: Tóth
authors.forename: Éva
authors.initials: É
authors.lastname: Juhász-Kaszanyitzky
authors.forename: Tünde
authors.initials: T
authors.lastname: Mag
authors.forename: Gabriella
authors.initials: G
authors.lastname: Hajbel-Vékony
authors.forename: Judit
authors.initials: J
authors.lastname: Pászti
authors.forename: Ivelina
authors.initials: I
authors.lastname: Damjanova
mesh_terms: D000818:Animals; D015373:Bacterial Typing Techniques; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology / Q000382:microbiology*; D004926:Escherichia coli / Q000145:classification / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification*; D004927:Escherichia coli Infections / Q000453:epidemiology / Q000382:microbiology* / Q000662:veterinary*; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D006814:Hungary / Q000453:epidemiology; D008969:Molecular Sequence Data; D010802:Phylogeny; D013552:Swine; D013553:Swine Diseases / Q000453:epidemiology / Q000382:microbiology*; D001618:beta-Lactamases / Q000235:genetics / Q000378:metabolism*
chemical_list: D029968:Escherichia coli Proteins; D001618:beta-Lactamases