title: Modulation of telomerase activity, bTERT and c-Myc induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin during Bovine Herpesvirus 1 infection in MDBK cells.
abstract: 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) influences infection of kidney cells (MDBK) with Bovine Herpesvirus 1 (BHV-1) through an increase in virus replication and an acceleration of BHV-1-induced apoptosis. Previously our group demonstrated that BHV-1, in the early stages of infection, significantly up-regulates telomerase activity in MDBK cells, while, in the late phases of infection, when BHV-1-induced apoptosis occurred, a down-regulation of telomerase activity was detected. Hence, herein, for the first time, we described the influences of TCDD on telomerase activity during virus infection. In kidney cells (MDBK) infected with BHV-1 and exposed to different doses of TCDD we explored telomerase activity by TRAP assay. Concomitantly, we examined protein levels of both bTERT and c-Myc by Western blot analysis. In all groups, TCDD induced an acceleration in down-regulation of telomerase activity. Particularly, TCDD drastically and significantly decreased telomerase activity when virus-induced apoptosis took place. This result was accompanied from an accelerated down-regulation of bTERT and c-Myc. Finally, in the presence of TCDD, we evidenced a dose-dependent overexpression of aryl hydrocarbon receptor. Hence, our data suggest that TCDD, through a significant acceleration in down-regulation of telomerase activity, bTERT and c-Myc, may contribute to accelerated BHV-1-induced apoptosis.
authors.affiliation: Department of Veterinary Medicine and Animal Production, University of Naples "Federico II", 80137 Naples, Italy. Electronic address: filomena.fiorito@unina.it.
authors.forename: Filomena
authors.initials: F
authors.lastname: Fiorito
authors.forename: Antonietta
authors.initials: A
authors.lastname: Cantiello
authors.forename: Giovanna Elvira
authors.initials: GE
authors.lastname: Granato
authors.forename: Gabriella
authors.initials: G
authors.lastname: Marfè
authors.forename: Roberto
authors.initials: R
authors.lastname: Ciarcia
authors.forename: Salvatore
authors.initials: S
authors.lastname: Florio
authors.forename: Ugo
authors.initials: U
authors.lastname: Pagnini
authors.forename: Luisa
authors.initials: L
authors.lastname: De Martino
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Iovane
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D015153:Blotting, Western; D002417:Cattle; D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D015536:Down-Regulation / Q000187:drug effects; D004785:Environmental Pollutants / Q000008:administration & dosage / Q000633:toxicity*; D006566:Herpesviridae Infections / Q000821:virology*; D007242:Herpesvirus 1, Bovine / Q000302:isolation & purification; D007668:Kidney / Q000166:cytology / Q000187:drug effects / Q000821:virology; D000072317:Polychlorinated Dibenzodioxins / Q000008:administration & dosage / Q000633:toxicity*; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics / Q000378:metabolism; D018336:Receptors, Aryl Hydrocarbon / Q000235:genetics; D019098:Telomerase / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism
chemical_list: D004785:Environmental Pollutants; D000072317:Polychlorinated Dibenzodioxins; D016271:Proto-Oncogene Proteins c-myc; D018336:Receptors, Aryl Hydrocarbon; D019098:Telomerase
keywords: 2,3,7,8-tetrachlorodibenzo-p-dioxin; AhR; Apoptosis; BHV-1; MDBK cells; Madin-Darby bovine kidney cells; TCDD; Telomerase activity; aryl hydrocarbon receptor; bTERT; bovine telomerase reverse transcriptase; c-Myc