title: Vasotocin analogues with selective natriuretic, kaliuretic and antidiuretic effects in rats.
abstract: The aim of the present study was an investigation of mechanisms mediating selective effect of vasotocin analogues on water, sodium, and potassium excretion. We tested vasotocin analogues: Mpa(1)-vasotocin (dAVT), Mpa(1)-Arg(4)-vasotocin (dAAVT) and Mpa(1)-DArg(8)-vasotocin (dDAVT). The effects on water, sodium, and potassium transport were evaluated in experiments using normal and water-loaded Wistar rats. It was shown that all tested peptides exerted antidiuretic activity. Vasotocin and dAVT induced natriuresis and kaliuresis in rats. V1a agonist (Phe(2)-Ile(3)-Orn(8)-vasopressin) reproduced the renal effects of dAVT on sodium and potassium excretion but not on water reabsorption. dAAVT, dDAVT and V2 agonist (desmopressin) induced kaliuresis without any effect on sodium excretion. Natriuresis was associated with increase in cGMP excretion, whereas kaliuresis was correlated with rise of cAMP excretion. V1a antagonist (Pmp(1)-Tyr(Me)(2)-vasopressin) significantly reduced the dAVT-stimulated natriuresis and did not influence on urinary potassium excretion. V2 antagonist (Pmp(1)-DIle(2)-Ile(4)-vasopressin) significantly reduced the dAVT- and dAAVT-induced kaliuresis. It is assumed that effects of the nonapeptides on sodium and potassium transport are independent of their antidiuretic activity and mediated by different subtypes of V receptors (the V1a or V1a-like receptor for natriuretic effect and V2 or V2-like one for kaliuretic). In accordance to the data obtained, there is a possibility of selective regulation of renal water reabsorption and urinary sodium and potassium excretion with involvement of neurohypophysial hormones.
authors.affiliation: Sechenov Institute of Evolutionary Physiology and Biochemistry of the Russian Academy of Sciences, 44 Thorez Pr., 194223 Saint-Petersburg, Russia. Electronic address: kutina_anna@mail.ru.
authors.forename: Anna V
authors.initials: AV
authors.lastname: Kutina
authors.forename: Anna S
authors.initials: AS
authors.lastname: Marina
authors.forename: Elena I
authors.initials: EI
authors.lastname: Shakhmatova
authors.forename: Yury V
authors.initials: YV
authors.lastname: Natochin
mesh_terms: D000818:Animals; D050034:Antidiuretic Agents / Q000494:pharmacology*; D065092:Antidiuretic Hormone Receptor Antagonists; D000242:Cyclic AMP / Q000652:urine; D006152:Cyclic GMP / Q000652:urine; D015232:Dinoprostone / Q000652:urine; D005260:Female; D007668:Kidney / Q000187:drug effects / Q000378:metabolism; D009318:Natriuresis / Q000187:drug effects; D011188:Potassium / Q000652:urine*; D051381:Rats; D017208:Rats, Wistar; D017483:Receptors, Vasopressin / Q000819:agonists / Q000378:metabolism; D012964:Sodium / Q000652:urine*; D014667:Vasopressins / Q000494:pharmacology; D014668:Vasotocin / Q000031:analogs & derivatives* / Q000494:pharmacology; D014867:Water / Q000378:metabolism
chemical_list: D050034:Antidiuretic Agents; D065092:Antidiuretic Hormone Receptor Antagonists; D017483:Receptors, Vasopressin; C587860:vasotocin, Mpa(1), Arg(4)-; C587861:vasotocin, Mpa(1)-; D014867:Water; D014667:Vasopressins; C038554:vasotocin, 1-(3-mercaptopropanoic acid)-8-Arg-; D012964:Sodium; D000242:Cyclic AMP; D006152:Cyclic GMP; D015232:Dinoprostone; D011188:Potassium; D014668:Vasotocin
keywords: Antidiuresis; Kaliuresis; Mpa(1)-Arg(4)-vasotocin; Mpa(1)-DArg(8)-vasotocin; Mpa(1)-vasotocin; Natriuresis; Vasopressin; Vasotocin analogues; dAAVT; dAVT; dDAVT