title: Detection of aac(6')-Ib-cr in avian pathogenic Escherichia coli isolates in Japan.
abstract: We investigated the prevalence of plasmid-mediated quinolone resistance (PMQR) genes in avian pathogenic Escherichia coli (APEC) strains in Japan. A total of 117 APEC strains collected between 2004 and 2007 were examined for PMQR genes (qnrA, qnrB, qnrC, qnrD, qnrS, aac(6')-Ib-cr, qepA and oqxAB) by polymerase chain reaction. None of the APEC strains carried qnrA, qnrB, qnrC, qnrD, qnrS, qepA or oqxAB, but one of the isolates was identified as an AAC (6')-Ib-cr producer. Phylogenetic grouping, multi-locus sequence typing and serotyping showed that this isolate belonged to phylogenetic group A, sequence type 167 and untypable serogroup. To our knowledge, this is the first report of the aac (6')-Ib-cr gene in bacteria from food-producing animals in Japan.
authors.affiliation: National Veterinary Assay Laboratory, Ministry of Agriculture, Forestry and Fisheries, 1-15-1 Tokura, Kokubunji, Tokyo 185-8511, Japan.
authors.forename: Michiko
authors.initials: M
authors.lastname: Kawanishi
authors.forename: Manao
authors.initials: M
authors.lastname: Ozawa
authors.forename: Mototaka
authors.initials: M
authors.lastname: Hiki
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Abo
authors.forename: Akemi
authors.initials: A
authors.lastname: Kojima
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Asai
mesh_terms: D000818:Animals; D002645:Chickens; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D004927:Escherichia coli Infections / Q000453:epidemiology / Q000235:genetics / Q000382:microbiology / Q000662:veterinary*; D007564:Japan / Q000453:epidemiology; D008826:Microbial Sensitivity Tests; D058885:Multilocus Sequence Typing / Q000662:veterinary; D010957:Plasmids / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000662:veterinary; D011201:Poultry Diseases / Q000453:epidemiology / Q000382:microbiology*; D016036:Seroepidemiologic Studies
chemical_list: D004269:DNA, Bacterial