title: Characterization of an extremely halotolerant Staphylococcus arlettae HPSSN35C isolated from Dwarka Beach, India.
abstract: An extremely halotolerant bacterium designated as HPSSN35C was isolated from saline soil of Dwarka beach, India. It exhibited growth over a wide range of NaCl in medium varying from 0 to 6 M. The isolate produced peach-pink pigment above ∼1.3 M NaCl. The culture was characterized using biochemical tests, bioMerieux Staph identification kit, API ID32 Staph system, and Biolog. Due to slow growth and extreme salt tolerance no ID was obtained in Biolog. Antibiotic sensitivity to various antibiotics was tested. Phenotypic characterization showed that it belonged to the novobiocin resistant staphylococci group. Analysis of 16S rRNA gene sequence comparison of 1452 base pairs showed that isolate is closely related to Staphylococcus saprophyticus group with close relationship to Staphylococcus arlettae (99% similarity). The halotolerant S. arlettae described in literature till date have been reported to tolerate 4.5 M NaCl and produce white to yellow pigment. The present study reports for the first time extremely halotolerant S. arlettae exhibiting growth up to ∼6 M NaCl and producing peach-pink pigment above ∼1.3 M NaCl.
authors.affiliation: Department of Biotechnology, Pramukh Swami Science and H. D. Patel Arts College, Kadi, Gujarat, India.
authors.forename: Sandhya G
authors.initials: SG
authors.lastname: Nanjani
authors.forename: Harsha P
authors.initials: HP
authors.lastname: Soni
mesh_terms: D015373:Bacterial Typing Techniques; D001483:Base Sequence; D024881:Drug Resistance, Bacterial; D007194:India; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D009675:Novobiocin / Q000494:pharmacology; D010802:Phylogeny; D010860:Pigments, Biological / Q000096:biosynthesis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D054712:Salinity; D055049:Salt Tolerance / Q000235:genetics*; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride / Q000378:metabolism; D012987:Soil / Q000737:chemistry; D012988:Soil Microbiology; D013210:Staphylococcus / Q000187:drug effects / Q000235:genetics / Q000254:growth & development* / Q000302:isolation & purification
chemical_list: D010860:Pigments, Biological; D012336:RNA, Ribosomal, 16S; D012987:Soil; D009675:Novobiocin; D012965:Sodium Chloride
keywords: Halotolerant; Saline soil; Staphylococci