title: Determination of storage time of saliva samples obtained from patients with and without chronic periodontitis for the comparison of some biochemical and cytological parameters.
abstract: BACKGROUND
  Salivary glutathione (GSH), malondialdehyde (MDA), protein, sialic acid (SA) levels, cytological parameters, and tissue factor activities (TFa) were investigated when fresh and after 3, 7, 11, 15, 21, and 30 days (d) of storage at -20°C both in the control and the periodontitis group. Moreover, the control and the periodontits groups were compared and continuity of the significances detected between the two groups were evaluated.
  
  
  METHODS
  GSH, MDA, SA, protein, and TFa were determined using the methods of Beutler, Yagi, Warren, Lowry, and Quick, respectively. Saliva imprint samples were stained with Giemsa and microscopically examined.
  
  
  RESULTS
  When the continuity of the significances of differences between the two groups was investigated, differences continued to be significant for GSH and TFa on days 3, 7, 11, 15, 21, and 30. Cytologically, only the significance detected between leucocyte numbers continued to be significant for 30 d. However significance of differences in total protein, MDA, and SA levels on day 0, were interrupted on days 3, 7, and 11, respectively.
  
  
  CONCLUSIONS
  Saliva samples may be stored for 30 d for GSH and TFa analyses in patients with and without periodontitis. However, to compare salivary MDA, SA, and total protein levels in these groups we suggest fresh samples to be studied.
authors.affiliation: Faculty of Dentistry, Department of Biochemistry, Marmara University, Istanbul, Turkey. ebruemekli@yahoo.com
authors.forename: Ebru
authors.initials: E
authors.lastname: Emekli-Alturfan
authors.forename: Aysen
authors.initials: A
authors.lastname: Yarat
authors.forename: Esin
authors.initials: E
authors.lastname: Çalışkan-Ak
authors.forename: Rabia
authors.initials: R
authors.lastname: Pisiriciler
authors.forename: Bahar
authors.initials: B
authors.lastname: Kuru
authors.forename: Ülkü
authors.initials: Ü
authors.lastname: Noyan
mesh_terms: D000328:Adult; D055113:Chronic Periodontitis / Q000503:physiopathology*; D004355:Drug Stability; D005615:Freezing; D005978:Glutathione / Q000032:analysis; D006801:Humans; D008315:Malondialdehyde / Q000032:analysis; D008875:Middle Aged; D019158:N-Acetylneuraminic Acid / Q000032:analysis; D012463:Saliva / Q000737:chemistry* / Q000166:cytology / Q000378:metabolism; D012471:Salivary Proteins and Peptides / Q000032:analysis; D013048:Specimen Handling / Q000379:methods*; D013925:Thromboplastin / Q000032:analysis
chemical_list: D012471:Salivary Proteins and Peptides; D008315:Malondialdehyde; D013925:Thromboplastin; D005978:Glutathione; D019158:N-Acetylneuraminic Acid
keywords: periodontitis; saliva; storage