title: Cellulose-based diagnostic devices for diagnosing serotype-2 dengue fever in human serum.
abstract: Here, two types of cellulose-based in vitro diagnostic devices are demonstrated for the diagnosis of dengue virus infection in both buffer system and human serum: 1) paper-based ELISA for providing the semiquantitative information of the disease activity of serotype-2 dengue fever to healthcare persons (i.e., monitoring the disease activity with a specific serotype in single patients); 2) lateral flow immunoassays to screen for infection with serotype-2 dengue fever (i.e., rapid YES or NO diagnosis prepared for large populations, in terms of global public health). Paper-based ELISA (specific to serotype-2 dengue fever), which builds off of our previous studies and a revised previous ELISA procedure, owns multiple advantages: 1) high sensitivity (about 40 times higher than the current ELISA-based approaches, due to our therapeutic-based monoclonal antibody) and specificity (specific to dengue virus serotype-2 nonstructural protein-1 antigens); 2) tiny amount of sample and reagent used for single tests; 3) short operating duration (i.e., rapid diagnostic device); and, 4) inexpensiveness (appropriate for use in all developing and underdeveloped nations of the world). Due to the higher sensitivity and shorter operating duration of paper-based ELISA (compared with conventional ELISA, and lateral flow immunoassays also performed in this study), this study has not only been able to perform the diagnosis of dengue virus serotype-2 nonstructural protein-1 antigens in both buffer system and human serum but also to evaluate dengue virus serotype-2 envelope proteins in the buffer system, thus successfully achieving the first such use of these proteins as the target antigen for the development of diagnostic tools. These results provide a more comprehensive understanding for the genesis of dengue fever diagnostic tools (through antibody-antigen recognition).
authors.affiliation: Institute of Nanoengineering and Microsystems, National Tsing Hua University, Hsinchu 30013, Taiwan; Institute of Cellular and Organismic Biology, Academia Sinica, Taipei 128, Taiwan.
authors.forename: Hsi-Kai
authors.initials: HK
authors.lastname: Wang
authors.forename: Cheng-Han
authors.initials: CH
authors.lastname: Tsai
authors.forename: Kuan-Hung
authors.initials: KH
authors.lastname: Chen
authors.forename: Chung-Tao
authors.initials: CT
authors.lastname: Tang
authors.forename: Jiun-Shyang
authors.initials: JS
authors.lastname: Leou
authors.forename: Pi-Chun
authors.initials: PC
authors.lastname: Li
authors.forename: Yin-Liang
authors.initials: YL
authors.lastname: Tang
authors.forename: Hsyue-Jen
authors.initials: HJ
authors.lastname: Hsieh
authors.forename: Han-Chung
authors.initials: HC
authors.lastname: Wu
authors.forename: Chao-Min
authors.initials: CM
authors.lastname: Cheng
mesh_terms: D002482:Cellulose / Q000737:chemistry*; D003715:Dengue / Q000097:blood* / Q000175:diagnosis*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006801:Humans; D007118:Immunoassay
chemical_list: D002482:Cellulose
keywords: cellulose-based ELISA; dengue fever; lateral flow immunoassays; rapid diagnosis