title: A tobacco etch virus protease with increased substrate tolerance at the P1' position.
abstract: Site-specific proteases are important tools for in vitro and in vivo cleavage of proteins. They are widely used for diverse applications, like protein purification, assessment of protein-protein interactions or regulation of protein localization, abundance or activity. Here, we report the development of a procedure to select protease variants with altered specificity based on the well-established Saccharomyces cerevisiae adenine auxotrophy-dependent red/white colony assay. We applied this method on the tobacco etch virus (TEV) protease to obtain a protease variant with altered substrate specificity at the P1' Position. In vivo experiments with tester substrates showed that the mutated TEV protease still efficiently recognizes the sequence ENLYFQ, but has almost lost all bias for the amino acid at the P1' Position. Thus, we generated a site-specific protease for synthetic approaches requiring in vivo generation of proteins or peptides with a specific N-terminal amino acid.
authors.affiliation: Department of Biology/Genetics, Philipps-Universität Marburg, Marburg, Germany.
authors.forename: Christian
authors.initials: C
authors.lastname: Renicke
authors.forename: Roberta
authors.initials: R
authors.lastname: Spadaccini
authors.forename: Christof
authors.initials: C
authors.lastname: Taxis
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000378:metabolism*; D010450:Endopeptidases / Q000737:chemistry / Q000378:metabolism*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D050505:Mutant Proteins / Q000378:metabolism; D009154:Mutation / Q000235:genetics; D059748:Proteolysis; D013379:Substrate Specificity
chemical_list: D000596:Amino Acids; D050505:Mutant Proteins; D010450:Endopeptidases; C099488:TEV protease