title: Application of attenuated total reflectance Fourier transform infrared spectroscopy for determination of cefixime in oral pharmaceutical formulations.
abstract: A quick and reliable analytical method for the quantitative assessment of cefixime in orally administered pharmaceutical formulations is developed by using diamond cell attenuated total reflectance (ATR) Fourier transform infrared (FT-IR) spectroscopy as an easy procedure for quality control laboratories. The standards for calibration were prepared in aqueous medium ranging from 350 to 6000mg/kg. The calibration model was developed based on partial least square (PLS) using finger print region of FT-IR spectrum in the range from 1485 to 887cm(-1). Excellent coefficient of determination (R(2)) was achieved as high as 0.99976 with root mean square error of 44.8 for calibration. The application of diamond cell (smart accessory) ATR FT-IR proves a reliable determination of cefixime in pharmaceutical formulations to assess the quality of the final product.
authors.affiliation: Pakistan Council of Scientific and Industrial Research Laboratories Complex, Karachi, Sindh, Pakistan. chemist_afi@yahoo.com
authors.forename: Aftab A
authors.initials: AA
authors.lastname: Kandhro
authors.forename: Abdul Hafeez
authors.initials: AH
authors.lastname: Laghari
authors.forename: Sarfaraz A
authors.initials: SA
authors.lastname: Mahesar
authors.forename: Rubina
authors.initials: R
authors.lastname: Saleem
authors.forename: Aisha
authors.initials: A
authors.lastname: Nelofar
authors.forename: Salman Tariq
authors.initials: ST
authors.lastname: Khan
authors.forename: S T H
authors.initials: ST
authors.lastname: Sherazi
mesh_terms: D000284:Administration, Oral; D002138:Calibration; D020682:Cefixime / Q000032:analysis* / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D004364:Pharmaceutical Preparations / Q000737:chemistry*; D011208:Powders / Q000737:chemistry; D012015:Reference Standards; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*; D013535:Suspensions / Q000737:chemistry
chemical_list: D004364:Pharmaceutical Preparations; D011208:Powders; D013535:Suspensions; D020682:Cefixime
keywords: Cefixime; Diamond cell-ATR; FT-IR; HPLC; PLS; Quality control