title: Identification of novel hypoxia response genes in human glioma cell line a172.
abstract: OBJECTIVE
  Hypoxia is a serious challenge for treatment of solid tumors. This condition has been manifested to exert significant therapeutic effects on glioblastoma multiform or (WHO) astrocytoma grade IV. Hypoxia contributes numerous changes in cellular mechanisms such as angiogenesis, metastasis and apoptosis evasion. Furthermore, in molecular level, hypoxia can cause induction of DNA breaks in tumor cells. Identification of mechanisms responsible for these effects can lead to designing more efficient therapeutic strategies against tumor progression which results in improvement of patient prognosis. Materials and Methods : In order to identify more hypoxia regulated genes which may have a role in glioblastoma progression, cDNA-AFLP was optimized as a Differential display method which is able to identify and isolate transcripts with no prior sequence knowledge.
  
  
  RESULTS
  Using this method, the current study identified 120 Transcription Derived Fragments (TDFs) which were completely differentially regulated in response to hypoxia. By sequence homology searching, the current study could detect 22 completely differentially regulated known genes and two unknown sequence matching with two chromosome contig and four sequence matches with some Expressed Sequence Tags (ESTs).
  
  
  CONCLUSIONS
  Further characterizing of these genes may help to achieve better understanding of hypoxia mediated phenotype change in tumor cells.
authors.affiliation: Department of Medical Genetics, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Baghbani
authors.forename: Reza
authors.initials: R
authors.lastname: Raoofian
authors.forename: Mohammad
authors.initials: M
authors.lastname: Hasanzadeh Nazarabadi
authors.forename: Tayebeh
authors.initials: T
authors.lastname: Hamzehloei
authors.forename: Mohammad
authors.initials: M
authors.lastname: Soukhtanloo
authors.forename: Mansur
authors.initials: M
authors.lastname: Heidari
authors.forename: Seyed Morteza
authors.initials: SM
authors.lastname: Afsharzadeh
authors.forename: Sahar
authors.initials: S
authors.lastname: Shekouhi
authors.forename: Fahimeh
authors.initials: F
authors.lastname: Moradi
authors.forename: Abdol-Azim
authors.initials: AA
authors.lastname: Sarli
authors.forename: Javad
authors.initials: J
authors.lastname: Zavar-Reza
authors.forename: Majid
authors.initials: M
authors.lastname: Mojarrad
keywords: Glioblastoma; Hypoxia; cDNA-AFLP