title: MRI-based analysis of intracerebral hemorrhage in mice reveals relationship between hematoma expansion and the severity of symptoms.
abstract: Intracerebral hemorrhage (ICH) is featured by poor prognosis such as high mortality rate and severe neurological dysfunction. In humans, several valuables including hematoma volume and ventricular expansion of hemorrhage are known to correlate with the extent of mortality and neurological dysfunction. However, relationship between hematoma conditions and the severity of symptoms in animal ICH models has not been clarified. Here we addressed this issue by using 7-tesla magnetic resonance imaging (MRI) on collagenase-induced ICH model in mice. We found that the mortality rate and the performance in behavioral tests did not correlate well with the volume of hematoma. In contrast, when hemorrhage invaded the internal capsule, mice exhibited high mortality and showed poor sensorimotor performance. High mortality rate and poor performance in behavioral tests were also observed when hemorrhage invaded the lateral ventricle, although worsened symptoms associated with ventricular hemorrhage were apparent only during early phase of the disease. These results clearly indicate that invasion of the internal capsule or the lateral ventricle by hematoma is a critical determinant of poor prognosis in experimental ICH model in mice as well as in human ICH patients. MRI assessment may be a powerful tool to refine investigations of pathogenic mechanisms and evaluations of drug effects in animal models of ICH.
authors.affiliation: Department of Chemico-Pharmacological Sciences, Graduate School of Pharmaceutical Sciences, Kumamoto University, Kumamoto, Japan.
authors.forename: Hideaki
authors.initials: H
authors.lastname: Matsushita
authors.forename: Masanori
authors.initials: M
authors.lastname: Hijioka
authors.forename: Akinori
authors.initials: A
authors.lastname: Hisatsune
authors.forename: Yoichiro
authors.initials: Y
authors.lastname: Isohama
authors.forename: Shigeto
authors.initials: S
authors.lastname: Iwamoto
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Terasawa
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Katsuki
mesh_terms: D000818:Animals; D002543:Cerebral Hemorrhage / Q000139:chemically induced / Q000401:mortality / Q000473:pathology*; D017364:Collagenases / Q000009:adverse effects; D018450:Disease Progression; D006406:Hematoma / Q000139:chemically induced / Q000401:mortality / Q000473:pathology*; D006801:Humans; D007276:Injections, Intraventricular; D020772:Internal Capsule / Q000187:drug effects / Q000473:pathology*; D020547:Lateral Ventricles / Q000187:drug effects / Q000473:pathology*; D008279:Magnetic Resonance Imaging; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D011597:Psychomotor Performance / Q000187:drug effects; D012720:Severity of Illness Index; D016019:Survival Analysis
chemical_list: D017364:Collagenases