title: Growth-promoting action and growth factor release by different platelet derivatives.
abstract: Abstract Platelet derivatives are commonly used in wound healing and tissue regeneration. Different procedures of platelet preparation may differentially affect growth factor release and cell growth. Preparation of platelet-rich fibrin (PRF) is accompanied by release of growth factors, including platelet-derived growth factor (PDGF), vascular endothelial growth factor (VEGF) and transforming growth factor β1 (TGFβ1), and several cytokines. When compared with the standard procedure for platelet-rich plasma (PRP), PRF released 2-fold less PDGF, but >15-fold and >2-fold VEGF and TGFβ1, respectively. Also, the release of several cytokines (IL-4, IL-6, IL-8, IL-10, IFNγ, MIP-1α, MIP-1β and TNFα) was significantly increased in PRF-conditioned medium (CM), compared to PRP-CM. Incubation of both human skin fibroblasts and human umbilical vein endothelial cells (HUVECs) with PRF-derived membrane (mPRF) or with PRF-CM enhanced cell proliferation by >2-fold (p<0.05). Interestingly, PRP elicited fibroblast growth at a higher extent compared to PRF. At variance, PRF effect on HUVEC growth was significantly greater than that of PRP, consistent with a higher concentration of VEGF in the PRF-CM. Thus, the procedure of PRP preparation leads to a larger release of PDGF, as a possible result of platelet degranulation, while PRF enhances the release of proangiogenic factors.
authors.affiliation: Department of Translational Medical Sciences, Federico II University of Naples , Naples , Italy .
authors.forename: F
authors.initials: F
authors.lastname: Passaretti
authors.forename: M
authors.initials: M
authors.lastname: Tia
authors.forename: V
authors.initials: V
authors.lastname: D'Esposito
authors.forename: M
authors.initials: M
authors.lastname: De Pascale
authors.forename: M
authors.initials: M
authors.lastname: Del Corso
authors.forename: R
authors.initials: R
authors.lastname: Sepulveres
authors.forename: D
authors.initials: D
authors.lastname: Liguoro
authors.forename: R
authors.initials: R
authors.lastname: Valentino
authors.forename: F
authors.initials: F
authors.lastname: Beguinot
authors.forename: P
authors.initials: P
authors.lastname: Formisano
authors.forename: G
authors.initials: G
authors.lastname: Sammartino
mesh_terms: D000328:Adult; D001792:Blood Platelets / Q000502:physiology*; D049109:Cell Proliferation / Q000187:drug effects; D016207:Cytokines / Q000378:metabolism; D005260:Female; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D061307:Human Umbilical Vein Endothelial Cells / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism* / Q000494:pharmacology; D008297:Male; D053657:Platelet-Rich Plasma; D055815:Young Adult
chemical_list: D016207:Cytokines; D036341:Intercellular Signaling Peptides and Proteins