title: An antigenic recombinant serine protease from Trichinella spiralis induces protective immunity in BALB/c mice.
abstract: In this study, we report the cloning and characterization of a cDNA encoding a Trichinella serine protease gene (TspSP-1.3) from GenBank. The recombinant TspSP-1.3 protein (rTspSP-1.3) was expressed in an Escherichia coli expression system and purified with Ni-affinity chromatography. Real-time quantitative PCR analysis revealed that TspSP-1.3 was expressed at significantly higher levels in muscle larvae and adult worms than in newborn larvae. TspSP-1.3 was detected in excretory-secretory proteins of Trichinella spiralis with western blotting. Immunization with the rTspSP-1.3 antigen induced humoral immune responses, which manifested as elevated specific anti-rTspSP-1.3 IgG and IgE antibodies and a mixed Th1/Th2 response. To determine whether purified rTspSP-1.3 had good antigenicity and could be a vaccine candidate for the control of T. spiralis infection, we immunized BALB/c mice with rTspSP-1.3 and subsequently challenged the mice with T. spiralis larvae. The results showed that mice vaccinated with rTspSP-1.3 exhibited an average reduction in the muscle larvae burden of 39 % relative to the control group. These results suggest that TspSP-1.3 could be a novel vaccine candidate for controlling Trichinella infection.
authors.affiliation: Sun Yat-sen University Zhongshan School of Medicine, Key Laboratory of Tropical Disease Control (Sun Yat-Sen University), Ministry of Education, 74 Zhongshan 2nd Road, Guangzhou, 510080, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Li
authors.forename: Jian-ping
authors.initials: JP
authors.lastname: Yao
authors.forename: Ai-hua
authors.initials: AH
authors.lastname: Pan
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.forename: Xu-chu
authors.initials: XC
authors.lastname: Hu
authors.forename: Zhong-dao
authors.initials: ZD
authors.lastname: Wu
authors.forename: Xing-wang
authors.initials: XW
authors.lastname: Zhou
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood*; D000947:Antigens, Helminth / Q000276:immunology*; D001483:Base Sequence; D018076:DNA, Complementary / Q000235:genetics; D005260:Female; D015801:Helminth Proteins / Q000235:genetics / Q000276:immunology*; D056724:Immunity, Humoral; D007114:Immunization; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D018510:RNA, Helminth / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar; D011994:Recombinant Proteins; D017422:Sequence Analysis, DNA; D057057:Serine Proteases / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D013047:Specific Pathogen-Free Organisms; D017160:Trichinella spiralis / Q000201:enzymology / Q000235:genetics / Q000276:immunology*; D014235:Trichinellosis / Q000469:parasitology / Q000517:prevention & control*
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D018076:DNA, Complementary; D015801:Helminth Proteins; D018510:RNA, Helminth; D011994:Recombinant Proteins; D057057:Serine Proteases