title: [Effect of resveratrol on the activation of murine CD4(+);T lymphocytes].
abstract: OBJECTIVE
  To study the effect of resveratrol on murine CD4(+);T lymphocyte activation.
  
  
  METHODS
  Splenic mononuclear cells (SMCs) from specific pathogen free (SPF) BALB/c mice (female, aged 9-10 weeks) were cultured with resveratrol (0, 10, 20, and 40 mmol/L) for 30 min in vitro, and then were activated with ConA, anti-CD3 or anti-CD3/anti-CD28. At 6 h (for CD69) and 36 h (for CD71), cells were harvested for being stained with anti-CD4-PerCP and anti-CD69-FITC, or anti-CD4-PerCP and anti-CD71-FITC, and the expressions of CD69 and CD71 were detected respectively using flow cytometry.
  
  
  RESULTS
  Resveratrol (≥10 mmol/L) significantly decreased CD69 levels on CD4(+);T lymphocytes activated with anti-CD3 in a dose-dependent manner (P<0.01). In addition, resveratrol (≥20 mmol/L) decreased CD69 levels on CD4(+);T lymphocytes activated with ConA, and anti-CD3/anti-CD28 in a dose-dependent manner (P<0.05). When concentrations ≥10 mmol/L, resveratrol depressed CD71 expression on ConA stimulated CD4(+);T lymphocytes (P<0.05), and resveratrol (≥20 mmol/L) decreased CD71 levels on anti-CD3 and anti-CD3/anti-CD28 stimulated CD4(+);T lymphocytes (P<0.05), all in a dose-dependent manner.
  
  
  CONCLUSIONS
  Resveratrol inhibits the activation of murine CD4(+);T lymphocytes in a dosedependent manner.
authors.affiliation: Department of Rheumatology, Second Affiliated Hospital, Guangzhou Medical College, Guangzhou 510260, China. wzhuolong@163.com
authors.forename: Zhuolong
authors.initials: Z
authors.lastname: Wang
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Zeng
authors.forename: Mengtao
authors.initials: M
authors.lastname: Li
authors.forename: Xiaofang
authors.initials: X
authors.lastname: Luo
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D000945:Antigens, Differentiation, T-Lymphocyte / Q000378:metabolism; D015496:CD4-Positive T-Lymphocytes / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D016130:Immunophenotyping; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D037181:Lectins, C-Type / Q000378:metabolism; D008213:Lymphocyte Activation / Q000187:drug effects* / Q000276:immunology; D008297:Male; D051379:Mice; D011990:Receptors, Transferrin / Q000378:metabolism; D000077185:Resveratrol; D013267:Stilbenes / Q000494:pharmacology*; D000099192:CD69 Antigens
chemical_list: D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D007166:Immunosuppressive Agents; D037181:Lectins, C-Type; D011990:Receptors, Transferrin; D000077185:Resveratrol; D013267:Stilbenes; D000099192:CD69 Antigens; C068126:CD71 antigen