title: Multiplexed cytokine profiling of serum for detection of colorectal cancer.
abstract: OBJECTIVE
  To evaluate the concentrations of eight cytokines in order to identify potential biomarkers for assisting in the detection of colorectal cancer.
  
  
  METHODS
  The concentrations of IFN-γ, IL-10, IL-6, IL-8, TNF-α, MMP-2, MMP-7 and MMP-9 were detected in the sera of 69 healthy controls, 93 colorectal adenoma patients and 149 colorectal cancer (CRC) patients.
  
  
  RESULTS
  Multivariate logistic regression analyses, which included CEA, CA199, IL-8, TNF-α and MMP-7, were used to evaluate the diagnostic value for differentiating between colorectal adenoma and CRC. The area under the curve was 0.945 (95% CI: 0.909-0.981). The sensitivity and specificity were 85.86 and 96.78%, respectively. Compared with the conventional biomarkers CEA and CA199, multivariate logistic regression showed significant improvement.
  
  
  CONCLUSIONS
  Our data demonstrated that testing using a panel of three serum cytokines, CEA and CA199 may have strong potential to assist in the detection of CRC.
authors.affiliation: Department of Clinical Biochemistry, State Key Laboratory of Kidney Disease, Chinese PLA General Hospital, Fuxin Road #28, Beijing, China.
authors.forename: Zhang
authors.initials: Z
authors.lastname: Pengjun
authors.forename: Wen
authors.initials: W
authors.lastname: Xinyu
authors.forename: Gu
authors.initials: G
authors.lastname: Feng
authors.forename: Deng
authors.initials: D
authors.lastname: Xinxin
authors.forename: Liu
authors.initials: L
authors.lastname: Yulan
authors.forename: Li
authors.initials: L
authors.lastname: Juan
authors.forename: Jia
authors.initials: J
authors.lastname: Xingwang
authors.forename: Dong
authors.initials: D
authors.lastname: Zhennan
authors.forename: Tian
authors.initials: T
authors.lastname: Yaping
mesh_terms: D000236:Adenoma / Q000097:blood*; D000328:Adult; D000368:Aged; D015295:Antigens, Tumor-Associated, Carbohydrate / Q000097:blood; D019540:Area Under Curve; D014408:Biomarkers, Tumor / Q000097:blood*; D002272:Carcinoembryonic Antigen / Q000097:blood; D016022:Case-Control Studies; D015179:Colorectal Neoplasms / Q000097:blood*; D016207:Cytokines / Q000097:blood*; D005260:Female; D006801:Humans; D016209:Interleukin-8 / Q000097:blood; D016015:Logistic Models; D008297:Male; D020783:Matrix Metalloproteinase 7 / Q000097:blood; D008875:Middle Aged; D011237:Predictive Value of Tests; D012680:Sensitivity and Specificity; D014409:Tumor Necrosis Factor-alpha / Q000097:blood; D055815:Young Adult
chemical_list: D015295:Antigens, Tumor-Associated, Carbohydrate; D014408:Biomarkers, Tumor; D002272:Carcinoembryonic Antigen; D016207:Cytokines; D016209:Interleukin-8; D014409:Tumor Necrosis Factor-alpha; C578959:carbohydrate antigen 199, human; C060932:MMP7 protein, human; D020783:Matrix Metalloproteinase 7