title: Clinical and microbiological features of cryptococcal meningitis.
abstract: BACKGROUND
  In this study, the clinical features, underlying diseases and clinical outcomes of patients with cryptococcosis were investigated. In addition, a molecular analysis of the Cryptococcus neoformans species complex isolated from these patients was performed.
  
  
  METHODS
  A prospective study of 62 cases of patients with cryptococcal infection was conducted at the Hospital de Doenças Tropicais de Goiás Dr. Anuar Auad from 2009-2010. Cryptococcal meningitis cases were diagnosed by direct examination and cerebrospinal fluid (CSF) sample culture. The profiling of these patients was assessed. The CSF samples were submitted to India ink preparation and cultured on Sabouraud dextrose agar, and C. neoformans was identified by the production of urease, a positive phenoloxidase test and assimilation of carbohydrates. C. neoformans and C. gattii isolates were distinguished by growth on L-canavanine-glycine-bromothymol blue medium, and molecular analysis was conducted via PCR fingerprinting reactions using M13 and (GACA)4 primers.
  
  
  RESULTS
  From the 62 patients with cryptococcosis, 71 isolates of CSF were obtained; 67 (94.4%) isolates were identified as C. neoformans var. grubii/VNI, and 4 (5.6%) were identified as C. gattii/VGII. Of these patients, 53 had an HIV diagnosis. The incidence of cryptococcosis was higher among patients 20-40 years of age, with 74.2% of the cases reported in males. Cryptococcus-related mortality was noted in 48.4% of the patients, and the symptoms were altered sensorium, headache, fever and stiff neck.
  
  
  CONCLUSIONS
  The high morbidity and mortality observed among patients with cryptococcosis demonstrate the importance of obtaining information regarding the epidemiological profile and clinical course of the disease in the State of Goiás, Brazil.
authors.affiliation: Laboratório de Micologia, Instituto de Patologia Tropical e Saúde Pública, Universidade Federal de Goias, Goiânia, GO, Brazil. lucia@padrao.com.br
authors.forename: Lúcia Kioko
authors.initials: LK
authors.lastname: Hasimoto e Souza
authors.forename: Carolina Rodrigues
authors.initials: CR
authors.lastname: Costa
authors.forename: Orionalda de Fátima Lisboa
authors.initials: Ode F
authors.lastname: Fernandes
authors.forename: Fernando Yano
authors.initials: FY
authors.lastname: Abrão
authors.forename: Thaisa Cristina
authors.initials: TC
authors.lastname: Silva
authors.forename: Carolina Martins
authors.initials: CM
authors.lastname: Treméa
authors.forename: Maria do Rosário Rodrigues
authors.initials: Mdo R
authors.lastname: Silva
mesh_terms: D000328:Adult; D001938:Brazil / Q000453:epidemiology; D003455:Cryptococcus neoformans / Q000235:genetics* / Q000302:isolation & purification; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000235:genetics; D005260:Female; D006801:Humans; D008297:Male; D016919:Meningitis, Cryptococcal / Q000382:microbiology* / Q000401:mortality; D008875:Middle Aged; D058889:Molecular Typing; D011446:Prospective Studies; D055815:Young Adult
chemical_list: D004269:DNA, Bacterial