title: Single KTP nanocrystals as second-harmonic generation biolabels in cortical neurons.
abstract: We report an efficient colloidal synthesis of KTiOPO4 (KTP) nanocrystals with excellent crystallinity and the direct observation of optical second-harmonic generation (SHG) from discrete KTP nanocrystals in neurons cultured from mammalian brain cortex. Direct internalization and monitoring of these nanoparticles was successfully achieved without limitations from cytotoxicity, bleaching and blinking emission.
authors.affiliation: Laboratoire de Physique de la Matière Condensée-Ecole Polytechnique-CNRS, UMR 7643, 91128 Palaiseau Cedex, France.
authors.forename: Ludovic
authors.initials: L
authors.lastname: Mayer
authors.forename: Abdallah
authors.initials: A
authors.lastname: Slablab
authors.forename: Géraldine
authors.initials: G
authors.lastname: Dantelle
authors.forename: Vincent
authors.initials: V
authors.lastname: Jacques
authors.forename: Aude-Marie
authors.initials: AM
authors.lastname: Lepagnol-Bestel
authors.forename: Sandrine
authors.initials: S
authors.lastname: Perruchas
authors.forename: Piernicola
authors.initials: P
authors.lastname: Spinicelli
authors.forename: Alice
authors.initials: A
authors.lastname: Thomas
authors.forename: Dominique
authors.initials: D
authors.lastname: Chauvat
authors.forename: Michel
authors.initials: M
authors.lastname: Simonneau
authors.forename: Thierry
authors.initials: T
authors.lastname: Gacoin
authors.forename: Jean-François
authors.initials: JF
authors.lastname: Roch
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology; D003102:Colloids / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry; D051379:Mice; D008856:Microscopy, Fluorescence; D053758:Nanoparticles / Q000737:chemistry*; D009474:Neurons / Q000737:chemistry* / Q000166:cytology; D010710:Phosphates / Q000737:chemistry*; D014025:Titanium / Q000737:chemistry*
chemical_list: D003102:Colloids; D005456:Fluorescent Dyes; D010710:Phosphates; C064806:potassium titanylphosphate; D014025:Titanium