title: A triggered DNA hydrogel cover to envelop and release single cells.
abstract: We develop an enzyme-triggered permeable DNA hydrogel cover to envelop and release single cells in microwells. The porous structure of the DNA hydrogel allows nutrients and waste to pass through, leading to a cell viability as high as 98%. The design provides a general method to culture, monitor, and manipulate single cells, and has potential applications in cell patterning and studying cell communication.
authors.affiliation: Key Laboratory of Organic Optoelectrics & Molecular Engineering of the Ministry of Education, Department of Chemistry, Tsinghua University, Beijing 100084, P. R. China, Fax: +86-10-62796082; National Center for Nanoscience and Technology, Beijing 100190, P. R. China; University of Chinese Academy of Sciences, Beijing 100049, P. R. China.
authors.forename: Juan
authors.initials: J
authors.lastname: Jin
authors.forename: Yongzheng
authors.initials: Y
authors.lastname: Xing
authors.forename: Yanli
authors.initials: Y
authors.lastname: Xi
authors.forename: Xueli
authors.initials: X
authors.lastname: Liu
authors.forename: Tao
authors.initials: T
authors.lastname: Zhou
authors.forename: Xinxin
authors.initials: X
authors.lastname: Ma
authors.forename: Zhongqiang
authors.initials: Z
authors.lastname: Yang
authors.forename: Shutao
authors.initials: S
authors.lastname: Wang
authors.forename: Dongsheng
authors.initials: D
authors.lastname: Liu
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques / Q000295:instrumentation; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D004262:DNA Restriction Enzymes / Q000378:metabolism; D005456:Fluorescent Dyes / Q000737:chemistry; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry*; D051379:Mice; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation; D018613:Microscopy, Confocal; D041681:NIH 3T3 Cells; D059010:Single-Cell Analysis
chemical_list: D005456:Fluorescent Dyes; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D004247:DNA; D004262:DNA Restriction Enzymes
keywords: DNA hydrogel; envelop and release; permeable; responsiveness; single cell