title: Synthesis, structural characterization, DNA binding studies and antitumor properties of tin(II)-oxydiacetate complexes containing α-diimine as auxiliary ligand.
abstract: Metal directed supra molecular assemblies with interesting topologies have been widely used as models for metallo-enzymes and in development of metallo-pharmaceuticals. Two novel tin(II)-oxydiacetate complexes with α-diimine (1,10-phenanthroline or 2,2'-bipyridine) as auxiliary ligand were synthesized and characterized by elemental analysis, FT-IR, (1)H-, (13)C- and (119)Sn-NMR and single crystal X-ray crystallography. The spectral investigations and X-ray data show that {Sn} is hepta coordinated with pentagonal bipyramidal (pbp) geometry of the complexes. The in vitro binding and cleavage studies using CT DNA by UV-visible, fluorescence and agarose gel electrophoresis techniques revealed that both complexes bind DNA via intercalation. The observed magnitudes of Kb for complexes (1) and (2) are 2.517×10(4) and 5.35×10(3), respectively, which suggest that (1) has strong binding affinity for CT DNA as compared to (2). The complexes were tested for antitumor properties and found highly active at 10(-4)M concentration against P388, HL-60 and A-549 cell lines.
authors.affiliation: Coordination Chemistry Research Group, Department of Chemistry, Aligarh Muslim University, Aligarh, India. zafarasiddiqi@gmail.com
authors.forename: Zafar A
authors.initials: ZA
authors.lastname: Siddiqi
authors.forename: Prashant K
authors.initials: PK
authors.lastname: Sharma
authors.forename: M
authors.initials: M
authors.lastname: Shahid
authors.forename: Mohd
authors.initials: M
authors.lastname: Khalid
mesh_terms: D015082:2,2'-Dipyridyl / Q000737:chemistry; D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry* / Q000494:pharmacology; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D056831:Coordination Complexes / Q000737:chemistry* / Q000494:pharmacology; D018360:Crystallography, X-Ray; D004247:DNA / Q000737:chemistry; D053837:DNA Cleavage; D006801:Humans; D008024:Ligands; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D010618:Phenanthrolines / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared; D014001:Tin / Q000737:chemistry* / Q000494:pharmacology
chemical_list: D000970:Antineoplastic Agents; D056831:Coordination Complexes; D008024:Ligands; D010618:Phenanthrolines; D015082:2,2'-Dipyridyl; D014001:Tin; D004247:DNA; C427203:calf thymus DNA; C025205:1,10-phenanthroline
keywords: Antitumor properties; Crystal structure; DNA binding; Tin(II)-oxydiacetate complex