title: Development of glycerol-utilizing Escherichia coli strain for the production of bioethanol.
abstract: The production of bioethanol was studied using recombinant Escherichia coli with glycerol as a carbon source. Glycerol is an attractive feedstock for biofuels production since it is generated as a major byproduct in biodiesel industry; therefore, we investigated the conversion of glycerol to bioethanol using E. coli BL21 (DE3) which harbors several genes in ethanol production pathway of Enterobacter aerogenes KCTC 2190. Fermentation was carried out at 34°C for 42h, pH 7.6, using defined production medium. Under optimal conditions, bioethanol production by the recombinant E. coli BL21 (DE3), strain pEB, was two-fold (3.01g/L) greater than that (1.45g/L) by the wild-type counterpart. The results obtained in this study will provide valuable guidelines for engineering bioethanol producers.
authors.affiliation: Department of Chemical and Biological Engineering, Korea University, 1-Anam-Dong, Seongbuk-Gu, Seoul, 136-701, Republic of Korea.
authors.forename: Laxmi Prasad
authors.initials: LP
authors.lastname: Thapa
authors.forename: Sang Jun
authors.initials: SJ
authors.lastname: Lee
authors.forename: Hah Young
authors.initials: HY
authors.lastname: Yoo
authors.forename: Han Suk
authors.initials: HS
authors.lastname: Choi
authors.forename: Chulhwan
authors.initials: C
authors.lastname: Park
authors.forename: Seung Wook
authors.initials: SW
authors.lastname: Kim
mesh_terms: D056804:Biofuels; D021902:Enterobacter aerogenes / Q000235:genetics / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation; D005798:Genes, Bacterial; D005818:Genetic Engineering / Q000379:methods; D005990:Glycerol / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D010957:Plasmids / Q000235:genetics; D013696:Temperature
chemical_list: D056804:Biofuels; D000431:Ethanol; D005990:Glycerol
keywords: Bioethanol production; Enterobacter aerogenes; Genetic manipulation; Recombinant strains