title: [Quantitative real-time PCR for target periodontal bacteria in subgingival plaque before and after local delivery of periocline, scaling and root planning].
abstract: OBJECTIVE
  To compare the copy number of Porphyromonas gingivalis (Pg) and Prevotella intermedia (Pi) in subgingival plaque before and after local delivery of periocline (2% minocycline hydrochloride ointment, MO), scaling and root planning (SRP) by quantitative real-time PCR (qRT-PCR) and evaluate the efficacy.
  
  
  METHODS
  Sixty-two adults with moderate to severe chronic periodontitis were selected in the study. Microbial samples were taken from pocket before and after MO and SRP(7d). The samples were evaluated by qRT-PCR for Pg and Pi. Microbiological effectiveness of treatments was assessed using Kruskal-Wallis and Wilcoxon rank-sum test. All tests were two-sided with a significance level of 0.05. All analyses were conducted with SAS 9.1.3 software package.
  
  
  RESULTS
  The copy number of Pg and Pi in subgingival plaque was 10(3)-10(6) and 10(2)-10(6). Bacterial loads of Pg were reduced in SPR+ MO, SRP and MO site. The counts of Pi decreased in SRP+ MO sites compared with those in the MO or SRP alone sites significantly (P<0.05).
  
  
  CONCLUSIONS
  Quantitative real-time PCR (qRT-PCR) is used as a powerful tool with high sensitivity and specificity to quantitatively assess target periodontal bacteria. The results show that subgingival administration of MO and SRP was effective for reducing pathogenic bacteria and improving clinical outcome. Supported by 2011 National Clinical Specialist Construction Project; Natural Science Foundation of Zhejiang Province(LY13H140002); Education Department Funds of Zhejiang Province(20061258) and Medical General Research Project of Zhejiang Province(2012KYB121).
authors.affiliation: Department of Conservative Dentistry, Zhejiang University, Hangzhou, China. dengshuli3@163.com
authors.forename: Shu-li
authors.initials: SL
authors.lastname: Deng
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.forename: Jia-yan
authors.initials: JY
authors.lastname: He
authors.forename: Zhuo
authors.initials: Z
authors.lastname: Chen
authors.forename: Hui
authors.initials: H
authors.lastname: Chen
mesh_terms: D000328:Adult; D001419:Bacteria; D001439:Bacteroides; D055113:Chronic Periodontitis; D003773:Dental Plaque; D012534:Dental Scaling; D006801:Humans; D010514:Periodontal Pocket; D010518:Periodontitis; D016966:Porphyromonas gingivalis; D018713:Prevotella intermedia; D060888:Real-Time Polymerase Chain Reaction; D016745:Root Planing