title: Evaluation of biodegradable polyester-co-lactone microparticles for protein delivery.
abstract: Abstract Poly(glycerol adipate-co-ω-pentadecalactone) (PGA-co-PDL) was previously evaluated for the colloidal delivery of α-chymotrypsin. In this article, the effect of varying polymer molecular weight (MW) and chemistry on particle size and morphology; encapsulation efficiency; in vitro release; and the biological activity of α-chymotrypsin (α-CH) and lysozyme (LS) were investigated. Microparticles were prepared using emulsion solvent evaporation and evaluated by various methods. Altering the MW or monomer ratio of PGA-co-PDL did not significantly affect the encapsulation efficiency and overall poly(1,3-propanediol adipate-co-ω-pentadecalactone) (PPA-co-PDL) demonstrated the highest encapsulation efficiency. In vitro release varied between polymers, and the burst release for α-CH-loaded microparticles was lower when a higher MW PGA-co-PDL or more hydrophobic PPA-co-PDL was used. The results suggest that, although these co-polyesters could be useful for protein delivery, little difference was observed between the different PGA-co-PDL polymers and PPA-co-PDL generally provided a higher encapsulation and slower release of enzyme than the other polymers tested.
authors.affiliation: School of Pharmacy and Biomolecular Sciences, Liverpool John Moores University , Liverpool , UK and.
authors.forename: Hesham M
authors.initials: HM
authors.lastname: Tawfeek
authors.forename: Sayed H
authors.initials: SH
authors.lastname: Khidr
authors.forename: Eman M
authors.initials: EM
authors.lastname: Samy
authors.forename: Sayed M
authors.initials: SM
authors.lastname: Ahmed
authors.forename: Elsie E
authors.initials: EE
authors.lastname: Gaskell
authors.forename: Gillian A
authors.initials: GA
authors.lastname: Hutcheon
mesh_terms: D002918:Chymotrypsin / Q000737:chemistry*; D016503:Drug Delivery Systems / Q000379:methods; D004655:Emulsions / Q000737:chemistry; D007783:Lactones / Q000737:chemistry*; D018942:Macrolides / Q000737:chemistry; D008863:Microspheres; D008970:Molecular Weight; D009113:Muramidase / Q000737:chemistry; D010316:Particle Size; D011091:Polyesters / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D011409:Propylene Glycols / Q000737:chemistry; D011506:Proteins / Q000737:chemistry*
chemical_list: D004655:Emulsions; D007783:Lactones; D018942:Macrolides; D011091:Polyesters; D011108:Polymers; D011409:Propylene Glycols; D011506:Proteins; C571591:omega-pentadecalactone; C568823:poly(glycerol adipate-co-omega-pentadecalactone); C041787:1,3-propanediol; D009113:Muramidase; D002918:Chymotrypsin; C117608:alpha-chymotrypsin
keywords: PGA-co-PDL; biodegradable polyesters; lysozyme; microparticles; protein delivery; α-Chymotrypsin