title: Potentiation of high hydrostatic pressure inactivation of Mycobacterium by combination with physical and chemical conditions.
abstract: Mycobacterium abscessus is an important hospital-acquired pathogen involved in infections associated with medical, surgical, and biopharmaceutical materials. In this work, we investigated the pressure-induced inactivation of two strains [2544 and American Type Culture Collection (ATCC) 19977] of M. abscessus in combination with different temperatures and pH conditions. For strain 2544, exposure to 250 MPa for 90 min did not significantly inactivate the bacteria at 20 °C, whereas at -15 °C, there was complete inactivation. Exposure to 250 MPa at ≥60 °C caused rapid inactivation, with no viable bacteria after 45 min. With 45 min of exposure, there were no viable bacteria at any temperature when a higher pressure (350 MPa) was used. Extremes of pH (4 or 9) also markedly enhanced the pressure-induced inactivation of bacteria at 250 MPa, with complete inactivation after 45 min. In comparison, exposure of this strain to the disinfecting agent glutaraldehyde (0.5 %) resulted in total inactivation within 5 min. Strain 19977 was more sensitive to high pressure but less sensitive to glutaraldehyde than strain 2544. These results indicate that high hydrostatic pressure in combination with other physical parameters may be useful in reducing the mycobacterial contamination of medical materials and pharmaceuticals that are sensitive to autoclaving.
authors.affiliation: Departamento de Bioquímica, Instituto de Biologia, Universidade Estadual de Campinas-UNICAMP, Rua Monteiro Lobato 255, Cidade Universitária Zeferino Vaz, 13083-862 Campinas, São Paulo, Brazil.
authors.forename: Ancelmo Rabelo
authors.initials: AR
authors.lastname: de Souza
authors.forename: Ana Luísa Silva Sampaio Moreira
authors.initials: AL
authors.lastname: da Costa Demonte
authors.forename: Karina
authors.initials: K
authors.lastname: de Araujo Costa
authors.forename: Mariana Alcantara Cardoso
authors.initials: MA
authors.lastname: Faria
authors.forename: Ricardo
authors.initials: R
authors.lastname: Durães-Carvalho
authors.forename: Marcelo
authors.initials: M
authors.lastname: Lancellotti
authors.forename: Carlos Francisco Sampaio
authors.initials: CF
authors.lastname: Bonafe
mesh_terms: D004203:Disinfection / Q000379:methods*; D005976:Glutaral / Q000633:toxicity; D006863:Hydrogen-Ion Concentration; D006874:Hydrostatic Pressure; D050296:Microbial Viability / Q000187:drug effects / Q000528:radiation effects; D009161:Mycobacterium / Q000187:drug effects / Q000502:physiology* / Q000528:radiation effects; D013696:Temperature; D013997:Time Factors
chemical_list: D005976:Glutaral