title: A proteomic study of spike development inhibition in bread wheat.
abstract: Spike development in wheat is a complicated development process and determines the wheat propagation and survival. We report herein a proteomic study on the bread wheat mutant strain 5660M underlying spike development inhibition. A total of 121 differentially expressed proteins, which were involved in cold stress response, protein folding and assembly, cell-cycle regulation, scavenging of ROS, and the autonomous pathway were identified using MS/MS and database searching. We found that cold responsive proteins were highly expressed in the mutant in contrast to those expressed in the wild-type line. Particularly, the autonomous pathway protein FVE, which modulates flowering, was dramatically downregulated and closely related to the spike development inhibition phenotype of 5660M. A quantitative RT-PCR study demonstrated that the transcription of the FVE and other six genes in the autonomous pathway and downstream flowering regulators were all markedly downregulated. The results indicate that spike development of 5660M cannot complete the floral transition. FVE might play an important role in the spikes development of the wheat. Our results provide the theory basis for studying floral development and transition in the reproductive growth period, and further analysis of wheat yield formation.
authors.affiliation: National Key Facilities for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, P. R. China; Crop Research Institute, Shandong Academy of Agricultural Sciences, Jinan, P. R. China.
authors.forename: Yong-Sheng
authors.initials: YS
authors.lastname: Zheng
authors.forename: Jun-Xian
authors.initials: JX
authors.lastname: Guo
authors.forename: Jin-Peng
authors.initials: JP
authors.lastname: Zhang
authors.forename: Ai-Nong
authors.initials: AN
authors.lastname: Gao
authors.forename: Xin-Ming
authors.initials: XM
authors.lastname: Yang
authors.forename: Xiu-Quan
authors.initials: XQ
authors.lastname: Li
authors.forename: Wei-Hua
authors.initials: WH
authors.lastname: Liu
authors.forename: Li-Hui
authors.initials: LH
authors.lastname: Li
mesh_terms: D002352:Carrier Proteins / Q000032:analysis* / Q000096:biosynthesis; D058639:Cold-Shock Response; D030562:Databases, Protein; D015536:Down-Regulation; D035264:Flowers / Q000196:embryology* / Q000254:growth & development; D018506:Gene Expression Regulation, Plant; D010940:Plant Proteins / Q000032:analysis*; D017510:Protein Folding; D040901:Proteomics / Q000379:methods*; D017382:Reactive Oxygen Species; D060888:Real-Time Polymerase Chain Reaction; D020539:Sequence Analysis, Protein; D053719:Tandem Mass Spectrometry; D014908:Triticum / Q000196:embryology* / Q000235:genetics / Q000254:growth & development
chemical_list: D002352:Carrier Proteins; D010940:Plant Proteins; D017382:Reactive Oxygen Species
keywords: 2DE; Autonomous pathway; Bread wheat; Cold reponses; Plant proteomics; Spike-development inhibition