title: The inhibition of 2,3-dichloro-1-propanol on T cell in vitro and in vivo.
abstract: 2,3-Dichloro-1-propanol (2,3-DCP) is a member of a group of chemicals known as chloropropanols. Currently, immunotoxicity of 2,3-DCP has not been reported. In the present study, we studied its inhibitory effects on T cell both in vivo and in vitro. The results showed that 2,3-DCP markedly inhibited ConA-induced splenocyte proliferation, Th1 and Th2 cytokine production, CD4(+) T cell populations, and the ratio of CD4(+)/CD8(+) T cells and cell cycle arrest in vitro. In addition, 2,3-DCP markedly suppressed DNFB-induced T-cell-mediated delayed-type hypersensitivity (DTH) reaction in mice. Furthermore, Western blot was used to study how 2,3-DCP affects signal transduction mechanisms. The data revealed that 2,3-DCP could down regulate activation of ConA-induced NF-κB and NFAT signal transduction pathways. These observations indicated that 2,3-DCP exhibited negative regulatory effects by directly suppressing T-cell-mediated immune responses in vitro and in vivo.
authors.affiliation: Jilin University, Changchun, Jilin, People's Republic of China.
authors.forename: Jing
authors.initials: J
authors.lastname: Lu
authors.forename: Guoren
authors.initials: G
authors.lastname: Huang
authors.forename: Shuang
authors.initials: S
authors.lastname: Zhang
authors.forename: Bocui
authors.initials: B
authors.lastname: Song
authors.forename: Zhenning
authors.initials: Z
authors.lastname: Wang
authors.forename: Linli
authors.initials: L
authors.lastname: Xu
authors.forename: Shuonan
authors.initials: S
authors.lastname: Zhang
authors.forename: Shuang
authors.initials: S
authors.lastname: Guan
mesh_terms: D000818:Animals; D016516:CD4-CD8 Ratio; D015496:CD4-Positive T-Lymphocytes / Q000187:drug effects* / Q000276:immunology; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D002728:Chlorohydrins / Q000008:administration & dosage* / Q000009:adverse effects; D016207:Cytokines / Q000378:metabolism*; D003602:Cytotoxicity, Immunologic / Q000187:drug effects; D005260:Female; D006968:Hypersensitivity, Delayed / Q000188:drug therapy* / Q000276:immunology; D007166:Immunosuppressive Agents / Q000008:administration & dosage* / Q000009:adverse effects; D008213:Lymphocyte Activation / Q000187:drug effects; D051379:Mice; D008807:Mice, Inbred BALB C; D016328:NF-kappa B / Q000378:metabolism; D050778:NFATC Transcription Factors / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D058408:Th1-Th2 Balance / Q000187:drug effects
chemical_list: D002728:Chlorohydrins; D016207:Cytokines; D007166:Immunosuppressive Agents; D016328:NF-kappa B; D050778:NFATC Transcription Factors; C076159:2,3-dichloro-1-propanol
keywords: 2,3-DCP; DTH; Inhibition; NF-κB; NFAT2; T-cell