title: Profilin1 sensitizes pancreatic cancer cells to irradiation by inducing apoptosis and reducing autophagy.
abstract: Pancreatic cancer has an extremely poor prognosis mainly due to lack of effective treatment options. Radiotherapy is mostly applied to locally advanced cases, although tumor radioresistance limits the effectiveness. Profilin1, a novel tumor suppressor gene, was reported to be down-regulated in various cancers and associated with tumor progression. The objective of this study was to demonstrate how profilin1 affected pancreatic cancer radiosensitivity. We showed profilin1 was down-regulated in pancreatic cancer cells after exposure to radiation, and re-expression of profilin1 suppressed tumor cell viability and increased DNA damage following irradiation. Further studies revealed that up-regulation of profilin1 facilitated apoptosis and repressed autophagy induced by irradiation, which might sensitize pancreatic cancer cells to radiation treatment. Our findings may provide a novel therapeutic strategy for sensitizing pancreatic cancer to radiotherapy.
authors.affiliation: Pancreatic Cancer Institute, Fudan University, P. R. China.
authors.forename: H
authors.initials: H
authors.lastname: Cheng
authors.forename: J
authors.initials: J
authors.lastname: Li
authors.forename: C
authors.initials: C
authors.lastname: Liu
authors.forename: W
authors.initials: W
authors.lastname: Yao
authors.forename: Y
authors.initials: Y
authors.lastname: Xu
authors.forename: T S
authors.initials: TS
authors.lastname: Frank
authors.forename: X
authors.initials: X
authors.lastname: Cai
authors.forename: S
authors.initials: S
authors.lastname: Shi
authors.forename: Y
authors.initials: Y
authors.lastname: Lu
authors.forename: Y
authors.initials: Y
authors.lastname: Qin
authors.forename: L
authors.initials: L
authors.lastname: Liu
authors.forename: J
authors.initials: J
authors.lastname: Xu
authors.forename: J
authors.initials: J
authors.lastname: Long
authors.forename: Q-X
authors.initials: QX
authors.lastname: Ni
authors.forename: M
authors.initials: M
authors.lastname: Li
authors.forename: X-J
authors.initials: XJ
authors.lastname: Yu
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000235:genetics* / Q000528:radiation effects*; D001343:Autophagy / Q000235:genetics / Q000528:radiation effects; D002460:Cell Line; D004249:DNA Damage / Q000528:radiation effects; D015972:Gene Expression Regulation, Neoplastic / Q000528:radiation effects; D006801:Humans; D051379:Mice; D010190:Pancreatic Neoplasms / Q000235:genetics* / Q000473:pathology; D051304:Profilins / Q000235:genetics*; D011836:Radiation Tolerance / Q000235:genetics*; D014965:X-Rays / Q000009:adverse effects; D023041:Xenograft Model Antitumor Assays
chemical_list: D051304:Profilins