title: DsRed2 transient expression in Culex quinquefasciatus mosquitoes.
abstract: Culex quinquefasciatus mosquitoes have been successfully genetically modified only once, despite the efforts of several laboratories to transform and establish a stable strain. We have developed a transient gene expression method, in Culex, that delivers plasmid DNA directly to the mosquito haemolymph and additional tissues. We were able to express DsRed2 fluorescent protein in adult Cx. quinquefasciatus mosquitoes by injecting plasmids directly into their thorax. The expression of DsRed2 in adult Cx. quinquefasciatus mosquitoes is an important stepping stone to genetic transformation and the potential use of new control strategies and genetic interactions.
authors.affiliation: Departamento de Epidemiologia, Faculdade de Saúde Pública, Universidade de São Paulo, São Paulo, SP, Brasil. andrebw.br@gmail.com
authors.forename: André Barretto Bruno
authors.initials: AB
authors.lastname: Wilke
authors.forename: Sarah
authors.initials: S
authors.lastname: Scaife
authors.forename: Luke
authors.initials: L
authors.lastname: Alphey
authors.forename: Mauro Toledo
authors.initials: MT
authors.lastname: Marrelli
mesh_terms: D000818:Animals; D003465:Culex / Q000235:genetics*; D015870:Gene Expression / Q000235:genetics*; D007303:Insect Vectors / Q000235:genetics*; D008164:Luminescent Proteins / Q000235:genetics*; D014170:Transformation, Genetic / Q000235:genetics*; D000097573:Red Fluorescent Protein
chemical_list: D008164:Luminescent Proteins; D000097573:Red Fluorescent Protein