title: Ursodeoxycholic acid-conjugated chitosan for photodynamic treatment of HuCC-T1 human cholangiocarcinoma cells.
abstract: Chitosan was hydrophobically modified with ursodeoxycholic acid (UDCA) to fabricate nano-photosensitizer for photodynamic therapy (PDT) of HuCC-T1 cholangiocarcinoma cells. Synthesis of UDCA-conjugated chitosan (ChitoUDCA) was confirmed using (1)H NMR spectra. Chlorin E6 (Ce6) was used as a photosensitizer and incorporated into ChitoUDCA nanoparticles through formation of ion complexes. Morphology of Ce6-incorporated ChitoUDCA nanoparticles was observed using TEM and their shapes were spherical with sizes around 200-400 nm. The PDT potential of Ce6-incorporated ChitoUDCA nanoparticles were studied with HuCC-T1 human cholangiocarcinoma cells. The results showed that ChitoUDCA nanoparticles enhances of Ce6 uptake into tumor cells, phototoxicity, and ROS generation compared to Ce6 itself. Furthermore, Ce6-incorporated ChitoUDCA nanoparticles showed quenching in aqueous solution and sensing at tumor cells. We suggest that Ce6-incorporated ChitoUDCA nanoparticles are promising candidates for PDT of cholangiocarcinoma cells.
authors.affiliation: National Research & Development Center for Hepatobiliary Cancer, Research Institute for Convergence of Biomedical Science and Technology, Pusan National University Yangsan Hospital, Gyeongnam 626-770, Republic of Korea.
authors.forename: Hye Myeong
authors.initials: HM
authors.lastname: Lee
authors.forename: Young-Il
authors.initials: YI
authors.lastname: Jeong
authors.forename: Do Hyung
authors.initials: DH
authors.lastname: Kim
authors.forename: Tae Won
authors.initials: TW
authors.lastname: Kwak
authors.forename: Chung-Wook
authors.initials: CW
authors.lastname: Chung
authors.forename: Cy Hyun
authors.initials: CH
authors.lastname: Kim
authors.forename: Dae Hwan
authors.initials: DH
authors.lastname: Kang
mesh_terms: D001650:Bile Duct Neoplasms / Q000378:metabolism / Q000473:pathology*; D001653:Bile Ducts, Intrahepatic / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D001692:Biological Transport; D045744:Cell Line, Tumor; D002626:Chemistry, Pharmaceutical; D048271:Chitosan / Q000737:chemistry*; D002735:Chlorophyllides; D018281:Cholangiocarcinoma / Q000378:metabolism / Q000473:pathology*; D004337:Drug Carriers; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D046529:Microscopy, Electron, Transmission; D010316:Particle Size; D010778:Photochemotherapy; D017319:Photosensitizing Agents / Q000737:chemistry / Q000494:pharmacology*; D011166:Porphyrins / Q000737:chemistry / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D013678:Technology, Pharmaceutical / Q000379:methods; D014580:Ursodeoxycholic Acid / Q000737:chemistry / Q000378:metabolism*
chemical_list: D002735:Chlorophyllides; D004337:Drug Carriers; D017319:Photosensitizing Agents; D011166:Porphyrins; D017382:Reactive Oxygen Species; C062985:phytochlorin; D014580:Ursodeoxycholic Acid; D048271:Chitosan
keywords: Chitosan; Chlorin e6; Cholangiocarcinoma; Photodynamic therapy; Ursodeoxycholic acid