title: In-depth study of homogeneity in DBS using two different techniques: results from the EBF DBS-microsampling consortium.
abstract: BACKGROUND
  At the start of their work, the European Bioanalysis Forum dried blood spots microsampling consortium did not form a dedicated team to investigate the spot homogeneity. However, two teams performed experiments that produced results relating to sample homogeneity.
  
  
  RESULTS
  The data, which were produced via two different approaches (a radiolabeled and a nonradiolabeled approach), are highly complementary and demonstrate clear effects on sample inhomogeneity due to the substrate type, compound and hematocrit levels.
  
  
  CONCLUSIONS
  The results demonstrate that sample inhomogeneity is a significant hurdle to the use of dried blood spots for regulated bioanalysis that should be investigated further in the method establishment phase if the whole spot is not sampled.
authors.affiliation: Quotient Bio Analytical Sciences (part of the LGC group), Newmarket Road, Fordham, Cambridgeshire, CB7 5WW, UK. zoe.cobb@quotientbioresearch.com
authors.forename: Zoe
authors.initials: Z
authors.lastname: Cobb
authors.forename: Ronald
authors.initials: R
authors.lastname: de Vries
authors.forename: Neil
authors.initials: N
authors.lastname: Spooner
authors.forename: Stephen
authors.initials: S
authors.lastname: Williams
authors.forename: Ludovicus
authors.initials: L
authors.lastname: Staelens
authors.forename: Mira
authors.initials: M
authors.lastname: Doig
authors.forename: Rebecca
authors.initials: R
authors.lastname: Broadhurst
authors.forename: Matthew
authors.initials: M
authors.lastname: Barfield
authors.forename: Nico
authors.initials: N
authors.lastname: van de Merbel
authors.forename: Bernhard
authors.initials: B
authors.lastname: Schmid
authors.forename: Christoph
authors.initials: C
authors.lastname: Siethoff
authors.forename: Jordi
authors.initials: J
authors.lastname: Ortiz
authors.forename: Elwin
authors.initials: E
authors.lastname: Verheij
authors.forename: Ben
authors.initials: B
authors.lastname: van Baar
authors.forename: Steve
authors.initials: S
authors.lastname: White
authors.forename: Philip
authors.initials: P
authors.lastname: Timmerman
mesh_terms: D000081:Acetamides / Q000097:blood; D000818:Animals; D002250:Carbon Radioisotopes; D003847:Deoxyglucose / Q000097:blood; D059788:Dried Blood Spot Testing / Q000592:standards* / Q000706:statistics & numerical data; D005060:Europe; D005161:Facility Regulation and Control; D006400:Hematocrit / Q000592:standards*; D006801:Humans; D007193:Independent Practice Associations; D000078334:Lacosamide; D051379:Mice; D051381:Rats; D015203:Reproducibility of Results; D013048:Specimen Handling / Q000592:standards*; D053719:Tandem Mass Spectrometry
chemical_list: D000081:Acetamides; D002250:Carbon Radioisotopes; D000078334:Lacosamide; D003847:Deoxyglucose