title: Bioinformatic analysis for structure and function of TCTP from Spirometra mansoni.
abstract: OBJECTIVE
  To predict structure and function of translationally controlled tumor protein (TCTP) from Spirometra mansoni by bioinformatics technology, and to provide a theoretical basis for further study.
  
  
  METHODS
  Open reading frame (ORF) of EST sequence from Spirometra mansoni was obtained by ORF finder and was translated into amino acid residue by DNAclub. The structure domain was analyzed by Blast. By the method of online analysis tools: Protparam, InterProScan, protscale, SignalP-3.0, PSORT II, BepiPred, TMHMM, VectorNTI Suite 9 packages and Phyre2, the structure and function of the protein were predicted and analyzed.
  
  
  RESULTS
  The results showed that the EST sequence was Sm TCTP with 173 amino acid residues, theoretical molecular weight was 19 872.0 Da. The protein has the closest evolutionary status with Clonorchis sinensis, Schistosoma mansoni, and Schistosoma japonicum. Then it had no signal peptide site and transmembrane domain. Secondary structure of TCTP contained two α -helices and eight β -strands.
  
  
  CONCLUSIONS
  Sm TCTP was a variety of biological functions of protein that may be used as a vaccine candidate molecule and drug target.
authors.affiliation: Department of Pathogen Biology, Hainan Medical University, Haikou, PR China. luyajuncc@163.com
authors.forename: Ya-Jun
authors.initials: YJ
authors.lastname: Lu
authors.forename: Gang
authors.initials: G
authors.lastname: Lu
authors.forename: Da-Zhong
authors.initials: DZ
authors.lastname: Shi
authors.forename: Li-Hua
authors.initials: LH
authors.lastname: Li
authors.forename: Sai-Feng
authors.initials: SF
authors.lastname: Zhong
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D014408:Biomarkers, Tumor / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D002590:Cestode Infections / Q000469:parasitology / Q000662:veterinary*; D019295:Computational Biology; D004283:Dog Diseases; D004285:Dogs; D020224:Expressed Sequence Tags; D015801:Helminth Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D006376:Helminths / Q000737:chemistry / Q000145:classification / Q000235:genetics; D016366:Open Reading Frames; D010802:Phylogeny; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D013146:Spirometra / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism / Q000469:parasitology*; D000090324:Tumor Protein, Translationally-Controlled 1
chemical_list: D014408:Biomarkers, Tumor; D015801:Helminth Proteins; D000090324:Tumor Protein, Translationally-Controlled 1
keywords: Bioinformatics analysis; Spirometra mansoni; Translationally controlled tumor protein