title: Aberrant promoter methylation of cell adhesion-related genes associated with clinicopathologic features in non-small cell lung cancer in China.
abstract: OBJECTIVE
  The aim of this study was to investigate the methylation status of three cell adhesion-related genes including CDH1, TSLC1 and TIMP3 in non-small cell lung cancer and explore its association with clinicopathologic features and various environmental risk factors.
  
  
  METHODS
  We detected the aberrant methylation presence of these genes by methylation-specific polymerase chain reaction and analyzed the potential correlations with multivariate logistic regression model as well as stepwise logistic regression.
  
  
  RESULTS
  For CDH1, promoter methylation was less frequent in adenosquamous carcinomas than adenocarcinomas (OR=0.35, 95%CI=0.13-0.96); pickled food increased the methylation frequency (OR=2.23, 95%CI=1.09-4.54) while light smoking and fruit intake decreased that (OR=0.43, 95%CI=0.19-0.97; OR=0.37, 95%CI=0.15-0.95). For TSLC1, males and toxin exposure increased methylation frequency (OR=6.25, 95%CI=1.05-37.13; OR=2.42, 95%CI=1.01-5.77) while light smoking and radiation exposure decreased that (OR=0.14, 95%CI=0.03-0.60; OR=0.17, 95%CI=0.04-0.87). For TIMP3, males showed lower methylation frequency than females (OR=0.18, 95%CI=0.04-0.88) while central lung cancer, heavy smoking and radiation exposure presented higher aberrant DNA methylation status (OR=2.19, 95%CI=1.07-4.52; OR=6.99, 95%CI=1.32-37.14; OR=2.30, 95%CI=1.04-5.08).
  
  
  CONCLUSIONS
  Aberrant promoter methylation of cell adhesion-related tumor suppressor genes in lung cancer displayed varieties of gene-specific correlations with clinicopathologic features and various environmental risk factors.
authors.affiliation: Environmental Epigenetics Laboratory, Department of Environmental Medicine, School of Medicine, Zhejiang University, Hangzhou, Zhejiang, China.
authors.forename: Pei-Wei
authors.initials: PW
authors.lastname: Xu
authors.forename: He-Yun
authors.initials: HY
authors.lastname: Xu
authors.forename: Xin-Neng
authors.initials: XN
authors.lastname: Liu
authors.forename: Chen-Ye
authors.initials: CY
authors.lastname: Zhang
authors.forename: Cong
authors.initials: C
authors.lastname: Tan
authors.forename: Chun-Mei
authors.initials: CM
authors.lastname: Chen
authors.forename: Hu
authors.initials: H
authors.lastname: Zhang
authors.forename: Yong-Tang
authors.initials: YT
authors.lastname: Jin
mesh_terms: D000328:Adult; D000368:Aged; D015703:Antigens, CD; D014408:Biomarkers, Tumor; D015820:Cadherins / Q000235:genetics; D002289:Carcinoma, Non-Small-Cell Lung / Q000209:etiology / Q000235:genetics* / Q000473:pathology*; D002448:Cell Adhesion / Q000235:genetics*; D000075982:Cell Adhesion Molecule-1; D015815:Cell Adhesion Molecules / Q000235:genetics; D019175:DNA Methylation; D005260:Female; D006801:Humans; D007136:Immunoglobulins / Q000235:genetics; D008175:Lung Neoplasms / Q000235:genetics* / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D011401:Promoter Regions, Genetic; D012307:Risk Factors; D019717:Tissue Inhibitor of Metalloproteinase-3 / Q000235:genetics
chemical_list: D015703:Antigens, CD; D014408:Biomarkers, Tumor; C403514:CADM1 protein, human; C513384:CDH1 protein, human; D015820:Cadherins; D000075982:Cell Adhesion Molecule-1; D015815:Cell Adhesion Molecules; D007136:Immunoglobulins; D019717:Tissue Inhibitor of Metalloproteinase-3