title: In vitro study of vancomycin release and osteoblast-like cell growth on structured calcium phosphate-collagen.
abstract: A drug delivery vehicle consisting of spherical calcium phosphate-collagen particles covered by flower-like (SFCaPCol) blossoms composed of nanorod building blocks and their cellular response is studied. The spherical structure was achieved by a combination of sonication and freeze-drying. The SFCaPCol blossoms have a high surface area of approximately 280 m(2) g(-1). The blossom-like formation having a high surface area allows a drug loading efficiency of 77.82%. The release profile for one drug, vancomycin (VCM), shows long term sustained release in simulated body fluid (SBF), in a phosphate buffer saline (PBS, pH 7.4) solution and in culture media over 2 weeks with a cumulative release ~53%, 75% and 50%, respectively, over the first 7 days. The biocompatibility of the VCM-loaded SFCaPCol scaffold was determined by in vitro cell adhesion and proliferation tests of rat osteoblast-like UMR-106 cells. MTT tests indicated that UMR-106 cells were viable after exposure to the VCM loaded SFCaPCol, meaning that the scaffold (the flower-like blossoms) did not impair the cell's viability. The density of cells on the substrate was seen to increase with increasing cultured time.
authors.affiliation: Department of Physics, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand. wponun@yahoo.com
authors.forename: Weeraphat
authors.initials: W
authors.lastname: Pon-On
authors.forename: Narattaphol
authors.initials: N
authors.lastname: Charoenphandhu
authors.forename: Jarinthorn
authors.initials: J
authors.lastname: Teerapornpuntakit
authors.forename: Jirawan
authors.initials: J
authors.lastname: Thongbunchoo
authors.forename: Nateetip
authors.initials: N
authors.lastname: Krishnamra
authors.forename: I-Ming
authors.initials: IM
authors.lastname: Tang
mesh_terms: D000327:Adsorption; D000818:Animals; D002130:Calcium Phosphates / Q000494:pharmacology*; D002417:Cattle; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D003094:Collagen / Q000494:pharmacology* / Q000648:ultrastructure; D007700:Kinetics; D046529:Microscopy, Electron, Transmission; D043942:Nanotubes / Q000737:chemistry / Q000648:ultrastructure; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects / Q000648:ultrastructure; D016062:Porosity; D051381:Rats; D017550:Spectroscopy, Fourier Transform Infrared; D013696:Temperature; D013997:Time Factors; D014640:Vancomycin / Q000494:pharmacology*
chemical_list: D002130:Calcium Phosphates; D014640:Vancomycin; D003094:Collagen; C020243:calcium phosphate
keywords: Calcium phosphate/collagen; Controlled drug release; Flower-like structure; Osteoblast-like cell growth