title: Interactions between hyaluronic acid, lysozyme, and the glucose oxidase-mediated lactoperoxidase system in enzymatic and candidacidal activities.
abstract: OBJECTIVE
  To investigate interactions between hyaluronic acid (HA), lysozyme, and the glucose oxidase-mediated lactoperoxidase (GO-LPO) system in enzymatic and candidacidal activities.
  
  
  METHODS
  The influences of HA (0.5, 1.0, and 2.0mg/mL) and lysozyme (30μg/mL hen egg white lysozyme) on the enzymatic activity of GO-LPO system (25μg/mL bovine LPO, 1mM KSCN, 10units/mL GO, and 30μg/mL glucose) were determined by measuring oxidized o-dianisidine production. The influence of the GO-LPO system on lysozyme activity was determined by measuring the turbidity of a Micrococcus lysodeikticus suspension. The effects of interactions between HA, lysozyme, the GO-LPO system on candidacidal activity were examined by pre-incubating various combinations of components. Candidacidal activity was determined by comparing the numbers of colony forming units using Candida albicans ATCC strains 10231, 18804, and 11006.
  
  
  RESULTS
  HA inhibited the enzymatic activity of the GO-LPO system in a dose-dependent manner. HA inhibited the candidacidal activities of the GO-LPO system. However, the inhibitory activity of HA was not significantly different according to concentration of HA. The GO-LPO system enhanced the enzymatic activity of lysozyme, though lysozyme did not affect the enzymatic activity of the GO-LPO system. The candidacidal activities of the GO-LPO system and lysozyme were not additive.
  
  
  CONCLUSIONS
  HA inhibited the enzymatic and candidacidal activity of the GO-LPO system. The GO-LPO system enhanced the enzymatic activity of lysozyme, but the candidacidal activities were not additive.
authors.affiliation: Department of Oral Medicine and Oral Diagnosis, School of Dentistry, Seoul National University, Seoul, Republic of Korea.
authors.forename: Min-Ah
authors.initials: MA
authors.lastname: Cho
authors.forename: Yoon-Young
authors.initials: YY
authors.lastname: Kim
authors.forename: Ji-Youn
authors.initials: JY
authors.lastname: Chang
authors.forename: Hong-Seop
authors.initials: HS
authors.lastname: Kho
mesh_terms: D000935:Antifungal Agents / Q000494:pharmacology*; D002176:Candida albicans / Q000187:drug effects*; D015169:Colony Count, Microbial; D004305:Dose-Response Relationship, Drug; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005949:Glucose Oxidase / Q000494:pharmacology*; D006820:Hyaluronic Acid / Q000494:pharmacology*; D007784:Lactoperoxidase / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D009113:Muramidase / Q000494:pharmacology*
chemical_list: D000935:Antifungal Agents; D004791:Enzyme Inhibitors; D006820:Hyaluronic Acid; D005949:Glucose Oxidase; D007784:Lactoperoxidase; C421864:hen egg lysozyme; D009113:Muramidase
keywords: Candidiacidal activity; Glucose oxidase; Hyaluronic acid; Lactoperoxidase; Lysozyme