title: Is CD36 gene polymorphism in region encoding lipid-binding domain associated with early onset CAD?
abstract: CD36 is a fatty acid translocase in striated muscle cells and cardiomyocytes. Some study suggested that alterations in CD36 gene may be associated with coronary artery disease (CAD) risk. The aim of the current study was to compare the frequency of CD36 variants in region encoding lipid-binding domain in Caucasian patients with early-onset CAD, no-CAD adult controls and neonates. The study group comprised 100 patients with early onset CAD. The genetic control groups were 306 infants and 40 no-CAD adults aged over 70years. Exons 4, 5 and 6 including fragments of flanking introns were studied using the denaturing high-performance liquid chromatography technique and direct sequencing. Changes detected in analyzed fragment of CD36: IVS3-6 T/C (rs3173798), IVS4-10 G/A (rs3211892), C311T (Thr104Ile, not described so far) in exon 5, G550A (Asp184Asn, rs138897347), C572T (Pro191Leu, rs143150225), G573A (Pro191Pro, rs5956) and A591T (Thr197Thr, rs141680676) in exon 6. No significant differences in the CD36 genotype, allele and haplotype frequencies were found between the three groups. Only borderline differences (p=0.066) were found between early onset CAD patients and newborns in the frequencies of 591T allele (2.00% vs 0.50%) and CGCGCGT haplotype (2.00% vs 0.50%) with both IVS3-6C and 591T variant alleles. In conclusion, CD36 variants: rs3173798, rs3211892, rs138897347, rs5956, rs143150225 rs141680676 and C311T do not seem to be involved in the risk of early-onset CAD in Caucasian population.
authors.affiliation: Department of Biochemistry and Medical Chemistry, Pomeranian Medical University, Szczecin, Poland. Electronic address: carmon12@gmail.com.
authors.forename: Monika
authors.initials: M
authors.lastname: Rać
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Safranow
authors.forename: Grzegorz
authors.initials: G
authors.lastname: Kurzawski
authors.forename: Andrzej
authors.initials: A
authors.lastname: Krzystolik
authors.forename: Dariusz
authors.initials: D
authors.lastname: Chlubek
mesh_terms: D000328:Adult; D000368:Aged; D018955:CD36 Antigens / Q000737:chemistry / Q000235:genetics*; D003324:Coronary Artery Disease / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D005091:Exons; D005260:Female; D005787:Gene Frequency; D056726:Genetic Association Studies; D005838:Genotype; D006239:Haplotypes; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D007438:Introns; D050356:Lipid Metabolism / Q000235:genetics*; D008055:Lipids / Q000235:genetics; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D012307:Risk Factors
chemical_list: D018955:CD36 Antigens; D008055:Lipids
keywords: A; Asn; Asp; BMI; C; C-reactive protein; CAD; CD36; Coronary artery disease; DHPLC; D′; ECG; FA; G; GWAS; Genetic association study; HDL; HapMap; IVS3; IVS4; Ile; International Haplotype Map Project; LCFA; LDL; LOD; LVMI; Leu; NCBI; National Center for Biotechnology Information; PCR; Polymorphism; Pro; Risk factors; ST; Single Nucleotide Polymorphism database; T; Thr; UTR; adenosine; asparagine; aspartic acid; body mass index; coronary artery disease; cytidine; dbSNP; denaturing high-performance liquid chromatography; electrocardiography; fatty acids; genome-wide association study; guanosine; high density lipoprotein; hsCRP; intron number 3; intron number 4; isoleucine; left ventricular mass index; leucine; logarithm of odds; long chain fatty acids; low density lipoprotein; name of gene encodes CD36 protein; polymerase chain reactions; proline; r(2); receptor, a fatty acid translocase; reference snip number; rs; score of linkage disequilibrium; the ST interval measured in electrocardiography; threonine; thymidine; unstranslated region