title: Jmjd3 controls mesodermal and cardiovascular differentiation of embryonic stem cells.
abstract: BACKGROUND
  The developmental role of the H3K27 demethylases Jmjd3, especially its epigenetic regulation at target genes in response to upstream developmental signaling, is unclear.
  
  
  OBJECTIVE
  To determine the role of Jmjd3 during mesoderm and cardiovascular lineage commitment.
  
  
  RESULTS
  Ablation of Jmjd3 in mouse embryonic stem cells does not affect the maintenance of pluripotency and self-renewal but compromised mesoderm and subsequent endothelial and cardiac differentiation. Jmjd3 reduces H3K27me3 marks at the Brachyury promoter and facilitates the recruitment of β-catenin, which is critical for Wnt signal-induced mesoderm differentiation.
  
  
  CONCLUSIONS
  These data demonstrate that Jmjd3 is required for mesoderm differentiation and cardiovascular lineage commitment.
authors.affiliation: From the Institute of Cardiovascular Regeneration, Centre for Molecular Medicine, University of Frankfurt, Frankfurt, Germany.
authors.forename: Kisho
authors.initials: K
authors.lastname: Ohtani
authors.forename: Cong
authors.initials: C
authors.lastname: Zhao
authors.forename: Gergana
authors.initials: G
authors.lastname: Dobreva
authors.forename: Yosif
authors.initials: Y
authors.lastname: Manavski
authors.forename: Britta
authors.initials: B
authors.lastname: Kluge
authors.forename: Thomas
authors.initials: T
authors.lastname: Braun
authors.forename: Michael A
authors.initials: MA
authors.lastname: Rieger
authors.forename: Andreas M
authors.initials: AM
authors.lastname: Zeiher
authors.forename: Stefanie
authors.initials: S
authors.lastname: Dimmeler
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002460:Cell Line; D019070:Cell Lineage; D053595:Embryonic Stem Cells / Q000166:cytology / Q000378:metabolism*; D042783:Endothelial Cells / Q000166:cytology; D004730:Endothelium, Vascular / Q000166:cytology*; D005326:Fetal Proteins / Q000235:genetics / Q000378:metabolism; D056484:Jumonji Domain-Containing Histone Demethylases / Q000235:genetics / Q000378:metabolism*; D008648:Mesoderm / Q000166:cytology* / Q000378:metabolism; D051379:Mice; D009154:Mutation; D032383:Myocytes, Cardiac / Q000166:cytology*; D011401:Promoter Regions, Genetic; D020825:T-Box Domain Proteins / Q000235:genetics / Q000378:metabolism; D060449:Wnt Signaling Pathway; D051176:beta Catenin / Q000378:metabolism; D000098908:Brachyury Protein
chemical_list: D005326:Fetal Proteins; D056484:Jumonji Domain-Containing Histone Demethylases; D020825:T-Box Domain Proteins; D051176:beta Catenin; D000098908:Brachyury Protein; C523761:Kdm6b protein, mouse
keywords: Brachyury protein; Jmjd3 protein, mouse; Wnt signaling pathway; embryonic stem cells; epigenomics; mesoderm