title: Titaniumcarboxonitride layer increased biocompatibility of medical polyetherurethanes.
abstract: Polyetherurethane (PEU) is in use for blood-contacted devices because of its excellent mechanical properties. However, poor hemocompatibility of the hydrophobic material required surface modification or endothelialization. To increase the biocompatibility of PEU, the polymer was coated with a titaniumcarboxonitride [Ti(C,N,O)] layer by a plasma-activated chemical vapor deposition (PACVD) process. Biocompatibility of titaniferously coated PEU was verified using static and dynamic cell culture techniques. Titaniferous coating significantly improved proliferation and mitochondrial activity of human endothelial cells on PEU. These cells captured significantly less mononuclear cells and platelets. Under shear stress for up to 72 hours, titaniferous coating increased endothelial cell adhesion, spreading, and cell density to form an organized monolayer covering the whole luminal surface of vascular PEU grafts. In summary, coating of PEU surfaces with Ti(C,N,O) might be a promising strategy to improve the biocompatibility of biomedical biomaterials.
authors.affiliation: Department of Cardiothoracic Surgery, University Medical Centre Regensburg, Franz-Josef-Strauss-Allee 11, 93042, Regensburg, Germany.
authors.forename: Sebastian
authors.initials: S
authors.lastname: Riescher
authors.forename: Daniel
authors.initials: D
authors.lastname: Wehner
authors.forename: Thomas
authors.initials: T
authors.lastname: Schmid
authors.forename: Hanngoerg
authors.initials: H
authors.lastname: Zimmermann
authors.forename: Björn
authors.initials: B
authors.lastname: Hartmann
authors.forename: Christof
authors.initials: C
authors.lastname: Schmid
authors.forename: Karla
authors.initials: K
authors.lastname: Lehle
mesh_terms: D002448:Cell Adhesion; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D042783:Endothelial Cells / Q000166:cytology / Q000378:metabolism; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D008422:Materials Testing; D008928:Mitochondria / Q000378:metabolism; D010973:Platelet Adhesiveness; D011140:Polyurethanes / Q000737:chemistry*; D011673:Pulsatile Flow; D013927:Thrombosis / Q000517:prevention & control; D014025:Titanium / Q000737:chemistry*
chemical_list: D020099:Coated Materials, Biocompatible; D011140:Polyurethanes; C115016:polyetherurethane; C096522:titanium carbonitride; D014025:Titanium
keywords: bioreactor; endothelial cell seeding; platelet adhesion; tissue engineering; titanium