title: The formate:oxygen oxidoreductase supercomplex of Escherichia coli aerobic respiratory chain.
abstract: The Escherichia coli formate:oxygen oxidoreductase supercomplex (FdOx) was investigated with respect to function and composition. Formate oxidoreductase activity was detected in blue native polyacrylamide gel electrophoresis (BN-PAGE) resolved membranes of E. coli, which were also capable of cyanide sensitive formate:oxygen oxidoreductase activity. The latter was compromised in strains devoid of specific oxygen reductases, particularly, in those devoid of cytochrome bo3 or bdI. A principal component analysis (PCA) integrating E. coli aerobic respiratory chain gene transcription, enzyme activity and growth dynamics was performed, correlating formate:oxygen oxidoreductase activity and the transcription of the genes encoding cytochromes bo3 and bdI, and corroborating previous evidence that associated these complexes in FdOx.
authors.affiliation: BIOTROP, Instituto de Investigação Científica Tropical, R. da Junqueira, 86, 1°, 1300-344 Lisboa, Portugal.
authors.forename: Pedro M F
authors.initials: PM
authors.lastname: Sousa
authors.forename: Marco A M
authors.initials: MA
authors.lastname: Videira
authors.forename: Ana M P
authors.initials: AM
authors.lastname: Melo
mesh_terms: D000332:Aerobiosis; D003486:Cyanides / Q000737:chemistry; D004579:Electron Transport; D004926:Escherichia coli / Q000201:enzymology*; D005560:Formate Dehydrogenases / Q000737:chemistry; D005561:Formates / Q000737:chemistry*; D009154:Mutation; D010088:Oxidoreductases / Q000737:chemistry*; D010100:Oxygen / Q000737:chemistry; D025341:Principal Component Analysis; D014158:Transcription, Genetic
chemical_list: D003486:Cyanides; D005561:Formates; C030544:formic acid; D010088:Oxidoreductases; D005560:Formate Dehydrogenases; D010100:Oxygen
keywords: 3-(N-Morpholino) propanesulfonic acid; AU; Aerobic respiratory chain supercomplex; BN-PAGE; Bacteria; Blue native polyacrylamide gel electrophoresis; DCPIP; DDM; Escherichia coli; FDH; FdOx; Formate:oxygen oxidoreductase activity; Gene transcription; K(3)[Fe(CN)(6)]; K(M); MOPS; Michaelis Menten constant; NADH:quinone oxidoreductase; NBT; NDH-1; NDH-2; OR; Oxygen reductase; PCA; PMS; UQ(1); V(MAX); alternative NADH:quinone oxidoreductase; arbitrary units; blue native polyacrylamide gel electrophoresis; coenzyme Q1, analogue to ubiquinone; deNADH; deamino-NADH; dichlorophenol indophenol; dodecyl-β-d-maltoside; formate dehydrogenase; formate:oxygen oxidoreductase supercomplex; maximal velocity; nitroblue tetrazolium; oxidoreductase; phenazine methosulfate; potassium ferricyanide; principal component analysis