title: [Synergistic protective effects of salvianolic acids and Panax notoginseng saponins on cardiomyocytes with hypoxia-reoxygenation injury].
abstract: OBJECTIVE
  To explore the mechanism of protective effects of salvianolic acids and Panax notoginseng saponins and their combination on cardiomyocytes suffered with hypoxia-reoxygenation (HR) injury.
  
  
  METHODS
  HR-injured H9c2 cell was employed as cellular model to evaluate cardioprotective effects of salvianolic acids, P. notoginseng saponins and their combination. The viability of cells was determined by MT assay, while the leakage of lactate dehydrogenase (LDH) was also determined. Apoptosis of cells was monitored by staining with Hoechst 33342 fluorescent, and was further evaluated by flow cytometry. Immunoblot analysis of apoptosis-related proteins Bcl-2, Bax and Caspase-3 was performed. Moreover, content of Na+, K(+)-ATPase, Ca2+, Mg2(+)-ATPase, and ATP were analyzed.
  
  
  RESULTS
  Salvianolic acids (0.05-0.5 mg x L(-1)) and P. notoginseng saponins (5-50 mg x L(-1)) have synergistic protective effects on cardiomyocytes with hypoxia-reoxygenation injury in a dose-dependent manner. Both salvianolic acids and P. notoginseng saponins can reduce hypoxia and reoxygenation-induced myocardial apoptosis and improve the energy metabolism in cardiomyocytes. Moreover, the combined use of salvianolic acids and P. notoginseng saponins exerts synergistic effects.
  
  
  CONCLUSIONS
  Salvianolic acids compatibility with P. notogiriseng saponins can protect cardiomyocytes during hypoxia and reoxygenation injury by inhibiting apoptosis and improving energy metabolism.
authors.affiliation: College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China. hfutgong56@163.com
authors.forename: Wan
authors.initials: W
authors.lastname: Gong
authors.forename: Yang
authors.initials: Y
authors.lastname: Xiao
authors.forename: Meng
authors.initials: M
authors.lastname: Zhang
authors.forename: Yi
authors.initials: Y
authors.lastname: Wang
authors.forename: Yi
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000475:Alkenes / Q000494:pharmacology*; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D015687:Cell Hypoxia / Q000187:drug effects; D002460:Cell Line; D004357:Drug Synergism; D032383:Myocytes, Cardiac / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism; D010100:Oxygen / Q000378:metabolism; D053723:Panax notoginseng / Q000737:chemistry*; D059808:Polyphenols / Q000494:pharmacology*; D051381:Rats; D012503:Saponins / Q000494:pharmacology*
chemical_list: D000475:Alkenes; D059808:Polyphenols; D012503:Saponins; C568740:salvianolic acid; D010100:Oxygen