title: Differential adaptive responses of Staphylococcus aureus to in vitro selection with different antimicrobial peptides.
abstract: We subjected Staphylococcus aureus ATCC 29213 to serial passage in the presence of subinhibitory concentrations of magainin 2 and gramicidin D for several hundred generations. We obtained S. aureus strains with induced resistance to magainin 2 (strain 55MG) and gramicidin D (strain 55GR) that showed different phenotypic changes in membrane properties. Both exhibited a change in membrane phospholipid content and an increase in membrane rigidity, while an alteration in net charge compared to that of the control occurred only in the case of 55MG.
authors.affiliation: School of Environmental Sciences, Jawaharlal Nehru University, New Delhi, India.
authors.forename: Tahsina
authors.initials: T
authors.lastname: Shireen
authors.forename: Madhuri
authors.initials: M
authors.lastname: Singh
authors.forename: Tiyasa
authors.initials: T
authors.lastname: Das
authors.forename: Kasturi
authors.initials: K
authors.lastname: Mukhopadhyay
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D000890:Anti-Infective Agents / Q000494:pharmacology*; D023181:Antimicrobial Cationic Peptides; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D006096:Gramicidin; D008826:Microbial Sensitivity Tests; D010455:Peptides / Q000494:pharmacology*; D010743:Phospholipids / Q000378:metabolism; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000378:metabolism*
chemical_list: D000900:Anti-Bacterial Agents; D000890:Anti-Infective Agents; D023181:Antimicrobial Cationic Peptides; D010455:Peptides; D010743:Phospholipids; D006096:Gramicidin