title: Acetone extract from Streptoverticillium sp., a bacterium isolated from Brazilian Cerrado soil, induces anti-inflammatory activity in mice.
abstract: The Streptoverticillium sp. Z1 is an actinomycete isolated from the soil under Cerrado vegetation, the extract of this strain was investigated in nociceptive and inflammatory models. The Streptoverticillium extract (ExS) 50 and 100 mg/kg (s.c.) produced a significant inhibition of acetic acid-induced abdominal writhings thereby demonstrating an anti-nociceptive effect. In the tail flick test the ExS (s.c.) was inactive. This result implited that ExS does not contain opioid-like compounds with central analgesic properties. In the inflammatory models, ExS 100 and 200 mg/kg (s.c.) were able to inhibit the croton oil-induced ear edema and, ExS 200 and 500 mg/kg (s.c.) inhibited the leukocyte migration on the carrageenan-induced peritonitis. The phospholipase A2 enzymatic assay showed that the anti-inflammatory activity of ExS was not due to direct effect on phospholipase A2 activity. These data suggest that Streptoverticillium sp. produces metabolites with anti-inflammatory effect and that these metabolites are unable to directly inhibit phospholipase A2 enzyme.
authors.affiliation: Laboratório de Farmacologia de Produtos Naturais, Departamento de Ciências Fisiológicas, Instituto de Ciências Biológicas, Universidade Federal de Goiás, 74001-970GoiâniaGO, Brasil.
authors.forename: Rodrigo B
authors.initials: RB
authors.lastname: Da Cruz
authors.forename: Pablinny M
authors.initials: PM
authors.lastname: Galdino
authors.forename: Karlla G B D
authors.initials: KG
authors.lastname: Penna
authors.forename: Karen
authors.initials: K
authors.lastname: Hoffmann
authors.forename: Elson A
authors.initials: EA
authors.lastname: Costa
authors.forename: Luiz A M
authors.initials: LA
authors.lastname: Bataus
mesh_terms: D000096:Acetone / Q000302:isolation & purification / Q000627:therapeutic use*; D000700:Analgesics / Q000302:isolation & purification / Q000627:therapeutic use*; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000302:isolation & purification / Q000627:therapeutic use*; D002351:Carrageenan; D003436:Croton Oil; D004487:Edema / Q000139:chemically induced / Q000188:drug therapy*; D008297:Male; D051379:Mice; D010146:Pain / Q000139:chemically induced / Q000188:drug therapy*; D012988:Soil Microbiology; D013306:Streptomycetaceae / Q000737:chemistry*
chemical_list: D000700:Analgesics; D000893:Anti-Inflammatory Agents; D000096:Acetone; D003436:Croton Oil; D002351:Carrageenan