title: Enhanced adhesion of osteoblastic cells on polystyrene films by independent control of surface topography and wettability.
abstract: We independently controlled surface topography and wettability of polystyrene (PS) films by CF4 and oxygen plasma treatments, respectively, to evaluate the adhesion and proliferation of human fetal osteoblastic (hFOB) cells on the films. Among the CF4 plasma-treated PS films with the average surface roughness ranging from 0.9 to 70 nm, the highest adhesion of hFOB cells was observed on a PS film with roughness of ~11 nm. When this film was additionally treated by oxygen plasma to provide a hydrophilic surface with a contact angle less than 10°, the proliferation of bone-forming cell was further enhanced. Thus, the plasma-based independent modification of PS film into an optimum nanotexture for human osteoblast cells could be appplied to materials used in bone tissue engineering.
authors.affiliation: National Creative Research Center for Block Copolymer Self-Assembly, Departments of Environmental Science & Engineering and Chemical Engineering, Pohang University of Science and Technology, Pohang, 790-784, South Korea.
authors.forename: Seung Yun
authors.initials: SY
authors.lastname: Yang
authors.forename: Eung-Sam
authors.initials: ES
authors.lastname: Kim
authors.forename: Gumhye
authors.initials: G
authors.lastname: Jeon
authors.forename: Kwan Yong
authors.initials: KY
authors.lastname: Choi
authors.forename: Jin Kon
authors.initials: JK
authors.lastname: Kim
mesh_terms: D002448:Cell Adhesion / Q000187:drug effects; D002478:Cells, Cultured; D006801:Humans; D018625:Microscopy, Atomic Force; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism; D010100:Oxygen / Q000494:pharmacology; D056951:Photoelectron Spectroscopy; D058626:Plasma Gases; D011137:Polystyrenes / Q000494:pharmacology*; D017155:Wettability / Q000187:drug effects
chemical_list: D058626:Plasma Gases; D011137:Polystyrenes; D010100:Oxygen
keywords: AFM; Bone remodeling; Cell adhesion; MW; Osteoblast; PS; Plasma surface modification; Polystyrene; RMS; SD; SEM; Surface roughness; X-ray photoelectron spectroscopy; XPS; atomic force microscope; hFOB; human fetal osteoblast; molecular weight; polystyrene; root mean square; scanning electron microscope; standard deviation