title: [Cloning and promoter analysis of gonadotrophin-releasing hormone Ⅱ in the orange-spotted grouper (Epinephelus coioides)].
abstract: Gonadotrophin-releasing hormone (GnRH) is a key regulator of reproduction in all vertebrates. We first cloned the cDNA and genomic DNA sequences coding for GnRHⅡ gene in the orange-spotted grouper (Epinephelis coioides), an economically important marine fish, and then cloned its promoter sequence. The region responsible for the cell-specific expression of GnRHⅡ was located between -2005 bp to -956 bp from the translation start site. GnRHⅡ promoter driven EGFP expression in transgenic zebrafish showed that GnRHⅡ-positive neurons were primarily located in the midbrain and in the eyes. Our results provide an improved understanding of the regulatory mechanism and function of GnRHⅡ of E. coioides.
authors.affiliation: Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, China. chenji7890@gmail.com
authors.forename: Ji
authors.initials: J
authors.lastname: Chen
authors.forename: Yu-Shan
authors.initials: YS
authors.lastname: Luo
authors.forename: Zuo-Yan
authors.initials: ZY
authors.lastname: Zhu
authors.forename: Wei
authors.initials: W
authors.lastname: Hu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D029941:Fish Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D007987:Gonadotropin-Releasing Hormone / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D008969:Molecular Sequence Data; D010473:Perciformes / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D011401:Promoter Regions, Genetic; D016415:Sequence Alignment
chemical_list: D029941:Fish Proteins; D007987:Gonadotropin-Releasing Hormone