title: Functionalization of the surface of electrospun poly(epsilon-caprolactone) mats using zwitterionic poly(carboxybetaine methacrylate) and cell-specific peptide for endothelial progenitor cells capture.
abstract: A novel approach for vascular grafts to achieve rapid endothelialization is to attract endothelial progenitor cells (EPCs) from peripheral blood onto grafts via EPC-specific antibodies, aptamer, or peptides that specifically bind to EPCs. Inspired by this idea, the electrospun poly(epsilon-caprolactone) (PCL) mats were modified with zwitterionic poly(carboxybetaine methacrylate) (PCBMA) and phage display-selected-EPC-specific peptide, TPSLEQRTVYAK (TPS). We tested the physical and chemical properties, cyto-compatibility, and platelet adhesion of the modified material, and investigated the specificity of the functionalized surface for capturing EPCs. The results indicated that PCL modified with zwitterionic PCBMA and TPS peptide showed improved hydrophilicity without morphology change and damage of the mats. Furthermore, the modified material supported adherence and growth of vascular cells and resisted platelets adhesion. The surfaces also specifically captured EPCs rather than bone marrow mesenchymal stem cells and human umbilical vein endothelial cells. This surface-functionalized PCL graft may offer new opportunities for designing new vascular grafts.
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology, Nankai University, Tianjin 300071, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Li
authors.forename: Zhihong
authors.initials: Z
authors.lastname: Wang
authors.forename: Suai
authors.initials: S
authors.lastname: Zhang
authors.forename: Wenting
authors.initials: W
authors.lastname: Zheng
authors.forename: Qiang
authors.initials: Q
authors.lastname: Zhao
authors.forename: Jun
authors.initials: J
authors.lastname: Zhang
authors.forename: Lianyong
authors.initials: L
authors.lastname: Wang
authors.forename: Shufang
authors.initials: S
authors.lastname: Wang
authors.forename: Deling
authors.initials: D
authors.lastname: Kong
mesh_terms: D000327:Adsorption; D000595:Amino Acid Sequence; D000818:Animals; D001622:Betaine / Q000737:chemistry*; D001792:Blood Platelets / Q000187:drug effects / Q000648:ultrastructure; D002470:Cell Survival / Q000187:drug effects; D042783:Endothelial Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D061307:Human Umbilical Vein Endothelial Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D041681:NIH 3T3 Cells; D010455:Peptides / Q000737:chemistry / Q000494:pharmacology*; D056951:Photoelectron Spectroscopy; D010973:Platelet Adhesiveness / Q000187:drug effects; D011091:Polyesters / Q000737:chemistry*; D011109:Polymethacrylic Acids / Q000737:chemistry*; D011817:Rabbits; D017550:Spectroscopy, Fourier Transform Infrared; D013234:Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D013499:Surface Properties / Q000187:drug effects; D014867:Water / Q000737:chemistry
chemical_list: D010455:Peptides; D011091:Polyesters; D011109:Polymethacrylic Acids; C517178:polycarboxybetaine methacrylate; D014867:Water; C016240:polycaprolactone; D001622:Betaine
keywords: Endothelial progenitor cells; Endothelialization; Platelet adhesion; Poly(epsilon-caprolactone); Zwitterions