title: Quantification of adipose tissue leukocytosis in obesity.
abstract: The infiltration of immune cell subsets in adipose tissue termed "adipose tissue leukocytosis" is a critical event in the development of chronic inflammation and obesity-associated comorbidities. Given that a significant proportion of cells in adipose tissue of obese patients are of hematopoietic lineage, the distinct adipose depots represent an uncharacterized immunological organ that can impact metabolic functions. Here, we describe approaches to characterize and isolate leukocytes from the complex adipose tissue microenvironment, to aid mechanistic studies to better understand the role of specific pattern recognition receptors (PRRs) such as inflammasomes in adipose-immune cross talk.
authors.affiliation: Immunobiology Laboratory, Pennington Biomedical Research Center, Louisiana State University System, Baton Rouge, LA, USA.
authors.forename: Ryan
authors.initials: R
authors.lastname: Grant
authors.forename: Yun-Hee
authors.initials: YH
authors.lastname: Youm
authors.forename: Anthony
authors.initials: A
authors.lastname: Ravussin
authors.forename: Vishwa Deep
authors.initials: VD
authors.lastname: Dixit
mesh_terms: D000273:Adipose Tissue / Q000473:pathology*; D000818:Animals; D005434:Flow Cytometry; D006801:Humans; D016130:Immunophenotyping; D050152:Intra-Abdominal Fat / Q000473:pathology; D007962:Leukocytes / Q000166:cytology / Q000378:metabolism; D007964:Leukocytosis / Q000175:diagnosis*; D051379:Mice; D009765:Obesity / Q000276:immunology / Q000473:pathology*