title: Cytauxzoon felis and 'Candidatus Mycoplasma haemominutum' coinfection in a Brazilian domestic cat (Felis catus).
abstract: This article describes the first detection of Cytauxzoon felis, using molecular techniques, in a naturally infected domestic cat from Brazil, South America. Coinfection with 'Candidatus Mycoplasma haemominutum' was also found. The molecular identification of the piroplasmid species was performed by Polymerase Chain Reaction (PCR) and sequencing analysis. A 284 pb fragment of the gene encoding the 18S ribosomal RNA region was amplified and showed 99% identity with other C. felis strains from North America. In addition, PCR-RFLP (restriction fragment length polymorphism) analysis, which amplifies a 595 bp fragment of the gene encoding 16S ribosomal RNA of some bacterial species, identified the co-infecting species as 'Candidatus M. haemominutum'.
authors.affiliation: Departamento de Patologia e Clínica Veterinária, Faculdade de Veterinária, Universidade Federal Fluminense, Niterói, RJ, Brazil.
authors.forename: Leticia Mendes Pupio
authors.initials: LM
authors.lastname: Maia
authors.forename: Aloysio de Mello Figueiredo
authors.initials: Ade M
authors.lastname: Cerqueira
authors.forename: Daniel
authors.initials: D
authors.lastname: de Barros Macieira
authors.forename: Aline Moreira
authors.initials: AM
authors.lastname: de Souza
authors.forename: Namir Santos
authors.initials: NS
authors.lastname: Moreira
authors.forename: Adrianna Vieira
authors.initials: AV
authors.lastname: da Silva
authors.forename: Joanne Belle
authors.initials: JB
authors.lastname: Messick
authors.forename: Renata Fernandes
authors.initials: RF
authors.lastname: Ferreira
authors.forename: Nádia Regina Pereira
authors.initials: NR
authors.lastname: Almosny
mesh_terms: D000818:Animals; D016782:Apicomplexa; D001938:Brazil; D002371:Cat Diseases / Q000382:microbiology* / Q000469:parasitology*; D002415:Cats; D060085:Coinfection; D009175:Mycoplasma Infections / Q000382:microbiology / Q000469:parasitology / Q000662:veterinary*; D057805:Pets; D011529:Protozoan Infections, Animal / Q000382:microbiology* / Q000469:parasitology*