title: Proteomic analysis of energy metabolism and signal transduction in irradiated melanoma cells.
abstract: OBJECTIVE
  To analyze proteomic and signal transduction alterations in irradiated melanoma cells.
  
  
  METHODS
  We combined stable isotope labeling with amino acids in cell culture (SILAC) with highly sensitive shotgun tandem mass spectrometry (MS) to create an efficient approach for protein quantification. Protein-protein interaction was used to analyze relationships among proteins.
  
  
  RESULTS
  Energy metabolism protein levels were significantly different in glycolysis and not significantly different in oxidative phosphorylation after irradiation. Conversely, tumor suppressor proteins related to cell growth and development were downregulated, and those related to cell death and cell cycle were upregulated in irradiated cells.
  
  
  CONCLUSIONS
  Our results indicate that irradiation induces differential expression of the 29 identified proteins closely related to cell survival, cell cycle arrest, and growth inhibition. The data may provide new insights into the pathogenesis of uveal melanoma and guide appropriate radiotherapy.
authors.affiliation: Department of Surgery, the Second Hospital of Lanzhou University, Lanzhou 730030, Gansu Province, China.
authors.forename: Lu-Bin
authors.initials: LB
authors.lastname: Yan
authors.forename: Kai
authors.initials: K
authors.lastname: Shi
authors.forename: Zhi-Tong
authors.initials: ZT
authors.lastname: Bing
authors.forename: Yi-Lan
authors.initials: YL
authors.lastname: Sun
authors.forename: Yang
authors.initials: Y
authors.lastname: Shen
keywords: 2D-LC-MS/MS; X-ray irradiation; melanoma cell; protein-protein interaction; proteomic analysis; stable isotope labeling with amino acids