title: [Effect of dendritic cells on the differentiation of Th1/Th17 in peripheral blood from preeclampsia patients].
abstract: OBJECTIVE
  To observe the effect of dendritic cells (DCs) derived from peripheral blood monouclear cells (PBMCs) on the differentiation of Th1 and Th17 in normal pregnancy women and preeclampsia patients.
  
  
  METHODS
  PBMCs were obtained from 32 preeclampsia patients and 20 normal pregnancy controls, respectively. Then DCs were sorted from peripheral blood monocytes cultured in the presence of cytokines (GM-CSF, IL-4) and LPS for 8 d. The phenotypes of DCs (CD14, CD80, CD83, CD86) were detected by flow cytometry (FCM). The content of IL-23 in the supernatant was detected by ELISA. CD4(+);T lymphocytes were separated using the magnetic BD IMag Cell Separation System according to the manufacturer's instructions. Purified CD4(+);T lymphocytes were clutured with mature DCs derived from normal pregnancy women (N-DC) and IL-2, or with mature DCs derived from preeclampsia patients (P-DC) and IL-2, or with N-DC and IL-1β, IL-6, or with P-DC and IL-1β, IL-6. At 6 days after culture, CD4(+);IFN-γ(+);T(Th1) and CD4(+);IL-17(+);T(Th17) subsets were determined by FCM.
  
  
  RESULTS
  Compared with N-DC, P-DC expressed the higher levels of CD83, CD80, CD86 and manifestated the stronger ability of promoting the differentiation of CD4(+);T into Th1/Th17 when cultured with different cytokines (P<0.01).
  
  
  CONCLUSIONS
  The changes in phenotype and function of DCs might be related to immune imbalance and be an important reason for preeclampsia.
authors.affiliation: Nantong Maternal and Child Health Hospital, Nantong 226006, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.forename: Liangxiang
authors.initials: L
authors.lastname: Su
authors.forename: Tianfeng
authors.initials: T
authors.lastname: Zhu
mesh_terms: D000328:Adult; D000945:Antigens, Differentiation, T-Lymphocyte / Q000378:metabolism; D002454:Cell Differentiation / Q000276:immunology*; D003713:Dendritic Cells / Q000276:immunology* / Q000378:metabolism; D005260:Female; D006801:Humans; D016130:Immunophenotyping; D007371:Interferon-gamma / Q000378:metabolism; D020381:Interleukin-17 / Q000378:metabolism; D053759:Interleukin-23 / Q000096:biosynthesis; D011225:Pre-Eclampsia / Q000276:immunology*; D011247:Pregnancy; D018417:Th1 Cells / Q000166:cytology* / Q000276:immunology / Q000378:metabolism; D058504:Th17 Cells / Q000166:cytology* / Q000276:immunology / Q000378:metabolism; D055815:Young Adult
chemical_list: D000945:Antigens, Differentiation, T-Lymphocyte; D020381:Interleukin-17; D053759:Interleukin-23; D007371:Interferon-gamma