title: A new blood collection device minimizes cellular DNA release during sample storage and shipping when compared to a standard device.
abstract: BACKGROUND
  Cell-free DNA (cfDNA) circulating in blood is currently used for noninvasive diagnostic and prognostic tests. Minimizing background DNA is vital for detection of low abundance cfDNA. We investigated whether a new blood collection device could reduce background levels of genomic DNA (gDNA) in plasma compared to K(3) EDTA tubes, when subjected to conditions that may occur during sample storage and shipping.
  
  
  METHODS
  Blood samples were drawn from healthy donors into K(3) EDTA and Cell-Free DNA™ BCT (BCT). To simulate shipping, samples were shaken or left unshaken. In a shipping study, samples were shipped or not shipped. To assess temperature variations, samples were incubated at 6°C, 22°C, and 37°C. In all cases, plasma was harvested by centrifugation and total plasma DNA (pDNA) assayed by quantitative real-time polymerase chain reaction (qPCR).
  
  
  RESULTS
  Shaking and shipping blood in K(3) EDTA tubes showed significant increases in pDNA, whereas no change was seen in BCTs. Blood in K(3) EDTA tubes incubated at 6°C, 22°C, and 37°C showed increases in pDNA while pDNA from BCTs remained stable.
  
  
  CONCLUSIONS
  BCTs prevent increases in gDNA levels that can occur during sample storage and shipping. This new device permits low abundance DNA target detection and allows accurate cfDNA concentrations.
authors.affiliation: Streck, Inc. Omaha, NE 68128, USA.
authors.forename: Sheila E
authors.initials: SE
authors.lastname: Norton
authors.forename: Kristin K
authors.initials: KK
authors.lastname: Luna
authors.forename: Joel M
authors.initials: JM
authors.lastname: Lechner
authors.forename: Jianbing
authors.initials: J
authors.lastname: Qin
authors.forename: M Rohan
authors.initials: MR
authors.lastname: Fernando
mesh_terms: D000925:Anticoagulants / Q000097:blood / Q000494:pharmacology; D001793:Blood Preservation; D001800:Blood Specimen Collection / Q000295:instrumentation*; D002474:Cell-Free System; D004247:DNA / Q000097:blood*; D004492:Edetic Acid / Q000097:blood / Q000494:pharmacology; D005260:Female; D006801:Humans; D008297:Male; D010949:Plasma; D013048:Specimen Handling; D013696:Temperature; D014186:Transportation
chemical_list: D000925:Anticoagulants; D004247:DNA; D004492:Edetic Acid
keywords: blood preservation; clinical laboratory techniques; hematology; investigative techniques; real-time polymerase chain reaction