title: [Influence of fibrinogen and laminin on bone mesenchymal stem cells osteogenic differentiation in PEGDA scaffold].
abstract: OBJECTIVE
  To explore the effects of fibrinogen(FG) and laminin(LN) in promoting the osteogenic differentiation of bone mesenchymal stem cells(BMSCs)in PEGDA scaffold.
  
  
  METHODS
  After the rabbit BMSCs were isolated and cultured to passage 3. BMSCs were blended in PEGDA-FG or PEGDA-LN hydrogels and cultured for 7 days. The levels of osterix,osteopontin,osteocalcin,collagen 2,myocardin,PPARΓ,and integrins Α2,Α5,and Α6 in PEGDA-FG and PEGDA-LN constructs were determined. Immunohistochemistry was used to detect the expressions of myocardin,PPARΓ,and OPN in PEGDA-FG and PEGDA-LN constructs.
  
  
  RESULTS
  The expressions of osterix,OPN,and OC were significantly higher in PEGDA-FG scaffold than day 0(all P<0.05). The OPN and OC expression levels were significantly higher in PEGDA-LN scaffold than day 0(both P<0.05). In PEGDA-FG and PEGDA-LN scaffold,myocardin,PPARΓ and COL 2 expression level showed no significant differences than day 0(all P>0.05). Integrin Α2 was upregulated in PEGDA-LN scaffold than day 0(P<0.05). Integrin Α6 was upregulated in PEGDA-FG scaffold than day 0(P<0.05). Immunohistochemistry stain showed that OPN expression increased in PEGDA-FG and PEGDA-LN scaffolds.
  
  
  CONCLUSIONS
  FG and LN can promote rabbit BMSCs osteogenic differentiation in PEGDA three-dimensional scaffold.
authors.affiliation: Department of Orthopedics, Zhujiang Hospital of Southern Medical University, Guangzhou, China.
authors.forename: Yin
authors.initials: Y
authors.lastname: Liu
authors.forename: Zhi
authors.initials: Z
authors.lastname: Zhan
authors.forename: Jing
authors.initials: J
authors.lastname: Tian
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000187:drug effects*; D002454:Cell Differentiation; D002478:Cells, Cultured; D005340:Fibrinogen / Q000494:pharmacology*; D007797:Laminin / Q000494:pharmacology*; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects*; D010012:Osteogenesis / Q000187:drug effects*; D011817:Rabbits; D054457:Tissue Scaffolds
chemical_list: D007797:Laminin; D005340:Fibrinogen