title: [Molecular identification of raw materials from lian qiao bai du wan].
abstract: Lian Qiao Bai Du Wan was used to study the identification of Chinese patent medicine by molecular marker technique. DNA was extracted through modified CTAB method. The psbA-trnH and rbcL sequences were gradient amplified, and PCR products were ligated with the pEASY-T5 vector and then transformed into Trans1-T1 cells, respectively. Clones were selected randomly and sequenced. All sequences were analyzed by BlastN and the neighbor-joining (NJ) phylogenetic tree was constructed by MEGA 4.0. The results showed that nine kinds of medicinal materials can be identified by psbA-trnH sequences, and six kinds of medicinal materials by rbcL sequences from Lian Qiao Bai Du Wan. Molecular marker technique can stably and accurately distinguish multi-origin medicinal materials in Chinese patent medicine.
authors.affiliation: Baotou Medical College, Inner Mongolia University of Science and Technology, Baotou 014060, China.
authors.forename: Zhan-Hu
authors.initials: ZH
authors.lastname: Cui
authors.forename: Chao
authors.initials: C
authors.lastname: Jiang
authors.forename: Min-Hui
authors.initials: MH
authors.lastname: Li
authors.forename: Min
authors.initials: M
authors.lastname: Chen
authors.forename: Li-She
authors.initials: LS
authors.lastname: Zhou
authors.forename: Yuan
authors.initials: Y
authors.lastname: Yuan
mesh_terms: D001483:Base Sequence; D002736:Chloroplasts / Q000235:genetics*; D016000:Cluster Analysis; D058893:DNA Barcoding, Taxonomic; D018742:DNA, Chloroplast / Q000235:genetics; D021901:DNA, Intergenic / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D031657:Forsythia / Q000737:chemistry* / Q000235:genetics; D010802:Phylogeny; D010946:Plants, Medicinal / Q000737:chemistry* / Q000235:genetics; D016133:Polymerase Chain Reaction; D012273:Ribulose-Bisphosphate Carboxylase / Q000235:genetics; D017422:Sequence Analysis, DNA; D013045:Species Specificity
chemical_list: D018742:DNA, Chloroplast; D021901:DNA, Intergenic; D018744:DNA, Plant; D004365:Drugs, Chinese Herbal; C101625:RbcL protein, plastid; D012273:Ribulose-Bisphosphate Carboxylase