title: Aristolochia debilis Sieb. et Zucc. induces apoptosis and reactive oxygen species in the HT-29 human colon cancer cell line.
abstract: In this study, we investigated the potential anticancer effect and mechanisms of action of a methanol extract from Aristolochia debilis Sieb. et Zucc (A. debilis) stems on human colon cancer cells (HT-29). A. debilis inhibited proliferation of HT-29 cells in dose-dependent and time-dependent manners as detected by the 1-(4,5-dimethylthiazol-2-yl)-3,5-diphenylformazan assay. A cell cycle analysis was performed via propidium iodide staining and flow cytometry. The A. debilis extract affected cell cycle progression by inducing sub-G1 arrest. The reactive oxygen species (ROS) level was determined by dichlorofluorescin diacetate. A decrease in the mitochondrial membrane potential and inhibition of manganese superoxide dismutase in combination with the accumulation of ROS induced apoptosis. Reverse transcription-polymerase chain reaction and Western blot analyses were used to determine changes in the expression of mitochondria-dependent apoptosis markers (Bax and Bcl-2). Upregulation of Bax and corresponding downregulation of Bcl-2 expression as well as ROS production may be the critical mechanism through which A. debilis induced apoptosis in HT-29 cells.
authors.affiliation: Department of Medical Biotechnology, Kangwon National University , Chuncheon-Si, South Korea .
authors.forename: Chunmei
authors.initials: C
authors.lastname: Li
authors.forename: Myeong-Hyeon
authors.initials: MH
authors.lastname: Wang
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D030022:Aristolochia / Q000737:chemistry*; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D003110:Colonic Neoplasms / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D057809:HEK293 Cells; D019073:HT29 Cells; D006801:Humans; D010936:Plant Extracts / Q000494:pharmacology*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000096:biosynthesis; D017382:Reactive Oxygen Species / Q000378:metabolism; D015398:Signal Transduction; D051028:bcl-2-Associated X Protein / Q000096:biosynthesis
chemical_list: C494826:BAX protein, human; D010936:Plant Extracts; D019253:Proto-Oncogene Proteins c-bcl-2; D017382:Reactive Oxygen Species; D051028:bcl-2-Associated X Protein