title: Role of angiogenesis in endometrial repair of patients with severe intrauterine adhesion.
abstract: OBJECTIVE
  To detect vascular endothelial growth factor (VEGF) expression and micro-vessel density (MVD) in patients with severe intrauterine adhesion before and after therapy, and to preliminarily explore the role of angiogenesis in the therapy of severe intrauterine adhesion.
  
  
  METHODS
  A total of 36 patients with severe intrauterine adhesion were prospectively recruited into the treatment group. In the control group, 20 patients with normal uterine were recruited. Patients with severe intrauterine adhesion received transcervical resection of adhesions under hysteroscope and then received artificial hormone therapy for 3 months.
  
  
  METHODS
  The changes in the organelles of endometrial cells were evaluated under an electric microscope; Immunohistochemistry was done to detect the VEGF expression and MVD in patients with severe intrauterine adhesion, which was compared with that in the control group; VEGF expression and MVD were compared among patients with different prognoses.
  
  
  RESULTS
  Electric microscopy showed vascular closure and hypoxic changes in the endometrial tissues of patients with intrauterine adhesion. After treatment, angiogenesis was observed, and the hypoxic changes in the endometrial glands and interstitium were also improved. Moreover, the VEGF expression and score of MVD also increased significantly when compared with those before treatment and in the control group. The VEGF expression and MVD score in intrauterine adhesion patients recovering from treatment were significantly higher than those in patients non-responding to treatment.
  
  
  CONCLUSIONS
  In patients with intrauterine adhesion, the endometrial tissues present with vascular closure, and angiogenesis will be present in the endometrial tissues after treatment. The angiogenesis in the endometrial tissues may affect the endometrial repair.
authors.affiliation: Department of Obstetrics and Gynecology, First Hospital, Sun Yat-sen University, Guangzhou, China. chyqing@mail.sysu.edu.cn
authors.forename: Yuqing
authors.initials: Y
authors.lastname: Chen
authors.forename: Yajie
authors.initials: Y
authors.lastname: Chang
authors.forename: Shuzhong
authors.initials: S
authors.lastname: Yao
mesh_terms: D000328:Adult; D002196:Capillaries / Q000473:pathology / Q000503:physiopathology; D016022:Case-Control Studies; D003131:Combined Modality Therapy; D004717:Endometrium / Q000098:blood supply* / Q000378:metabolism / Q000648:ultrastructure; D004958:Estradiol / Q000008:administration & dosage / Q000031:analogs & derivatives*; D005260:Female; D006801:Humans; D015907:Hysteroscopy; D017258:Medroxyprogesterone Acetate / Q000008:administration & dosage*; D018919:Neovascularization, Physiologic; D011446:Prospective Studies; D012720:Severity of Illness Index; D000267:Tissue Adhesions; D016896:Treatment Outcome; D015854:Up-Regulation; D014591:Uterine Diseases / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology / Q000628:therapy*; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism; D055815:Young Adult
chemical_list: C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A; D004958:Estradiol; D017258:Medroxyprogesterone Acetate
keywords: Intrauterine adhesion; angiogenesis; micro-vessel density; vascular endothelial growth factor