title: Differential gene-expression analysis defines a molecular pattern related to olive pollen allergy.
abstract: Analysis of gene-expression profiles by microarrays is useful for characterization of candidate genes, key regulatory networks, and to define phenotypes or molecular signatures which improve the diagnosis and/or classification of the allergic processes. We have used this approach in the study of olive pollen response in order to find differential molecular markers among responders and non-responders to this allergenic source. Five clinical groups, non-allergic, asymptomatic, allergic but not to olive pollen, untreated-olive-pollen allergic patients and olive-pollen allergic patients (under specific-immunotherapy), were assessed during and outside pollen seasons. Whole-genome gene expression analysis was performed in RNAs extracted from PBMCs. After assessment of data quality and principal components analysis (PCA), differential gene-expression, by multiple testing and, functional analyses by KEGG, for pathways and Gene-Ontology for biological processes were performed. Relevance was defined by fold change and corrected P values (less than 0.05). The most differential genes were validated by qRT-PCR in a larger set of individuals. Interestingly, gene-expression profiling obtained by PCA clearly showed five clusters of samples that correlated with the five clinical groups. Furthermore, differential gene expression and functional analyses revealed differential genes and pathways in the five clinical groups. The 93 most significant genes found were validated, and one set of 35 genes was able to discriminate profiles of olive pollen response. Our results, in addition to providing new information on allergic response, define a possible molecular signature for olive pollen allergy which could be useful for the diagnosis and treatment of this and other sensitizations.
authors.affiliation: Immunology Department, IIS-Fundacion Jimenez Diaz, Madrid, Spain.
authors.forename: M
authors.initials: M
authors.lastname: Aguerri
authors.forename: D
authors.initials: D
authors.lastname: Calzada
authors.forename: D
authors.initials: D
authors.lastname: Montaner
authors.forename: M
authors.initials: M
authors.lastname: Mata
authors.forename: F
authors.initials: F
authors.lastname: Florido
authors.forename: J
authors.initials: J
authors.lastname: Quiralte
authors.forename: J
authors.initials: J
authors.lastname: Dopazo
authors.forename: C
authors.initials: C
authors.lastname: Lahoz
authors.forename: B
authors.initials: B
authors.lastname: Cardaba
mesh_terms: D000328:Adult; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D008297:Male; D008875:Middle Aged; D031658:Olea / Q000276:immunology*; D025341:Principal Component Analysis; D006255:Rhinitis, Allergic, Seasonal / Q000235:genetics*