title: Association between toll-like receptor2 Arg677Trp and 597T/C gene polymorphisms and pulmonary tuberculosis in Zahedan, Southeast Iran.
abstract: BACKGROUND
  It is well known that toll-like receptor 2 (TLR2) mediates responses of both innate and adaptive immunity to microbial pathogen, including mycobacteria. Single-nucleotide polymorphisms (SNPs) in the TLR2 gene that impair its function may be associated with the development of pulmonary tuberculosis (PTB). The aim of this study was to evaluate the possible association between TLR2 Arg677Trp and 597T/C polymorphisms and PTB in a sample of Iranian population.
  
  
  METHODS
  This case-control study was performed on 174 PTB and 177 healthy subjects. Tetra amplification refractory mutation system-polymerase chain reaction (T-ARMS-PCR) was used to detect the SNPs.
  
  
  RESULTS
  There was no significant difference in the polymorphism of Arg677Trp of the TLR2 gene among PTB and control groups (p>0.05). The results showed that there was a significant difference between case and control groups regarding 597T/C polymorphism (χ(2)=12.21, p=0.002). The TC and CC genotypes were found to be associated with the risk of PTB (OR=2.13, 95% CI=1.25-3.62, p=0.005 and OR=4.88, 95% CI=1.56-15.26, p=0.007, respectively).
  
  
  CONCLUSIONS
  Our data suggest that 597T/C polymorphism, but not Arg677Trp polymorphism, of the TLR-2 gene is a risk factor for susceptibility to PTB in a sample of Iranian population.
authors.affiliation: Research Center for Infectious Diseases and Tropical Medicine, Zahedan University of Medical Sciences, Zahedan, Iran.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Naderi
authors.forename: Mohammad
authors.initials: M
authors.lastname: Hashemi
authors.forename: Leylisadat
authors.initials: L
authors.lastname: Hazire-Yazdi
authors.forename: Mohsen
authors.initials: M
authors.lastname: Taheri
authors.forename: Abdolkarim
authors.initials: A
authors.lastname: Moazeni-Roodi
authors.forename: Ebrahim
authors.initials: E
authors.lastname: Eskandari-Nasab
authors.forename: Gholamreza
authors.initials: G
authors.lastname: Bahari
mesh_terms: D016022:Case-Control Studies; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D005838:Genotype; D006801:Humans; D007492:Iran; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D012307:Risk Factors; D051195:Toll-Like Receptor 2 / Q000235:genetics*; D014397:Tuberculosis, Pulmonary / Q000235:genetics*
chemical_list: D051195:Toll-Like Receptor 2
keywords: Single nucleotide polymorphism; TLR2; Tuberculosis