title: Comparison of α2-macroglobulin synthesis by juvenile vs. mature rats after identical inflammatory stimulation.
abstract: Synthesis of α2-macoglobulin (α2M) by 3-week-old juvenile rats was compared to that of mature 7- and 11-week-old rats. Serum concentrations of α2M, interleukin (IL)-6- and cytokine-induced neutrophil chemoattractant (CINC)-1 were measured by enzyme-linked immunosorbent assay. The area under the concentration vs. time curve (AUC) for α2M was significantly different among the three groups. The synthesis of α2M increased in an age-dependent manner. No significant difference was observed for the AUC of IL-6, but that of CINC-1 in 3-week-old rats was significantly lower than that in 7- or 11-week-old rats. These results suggest that synthesis of α2M was increased in mature compared to juvenile rats, possibly due to differences in liver function. The maximum concentration of CINC-1 in 3-week-old rats was observed 6 h after turpentine oil injection. The serum concentrations of IL-6 and CINC-1 increased more quickly in juvenile rats than in mature rats after inflammatory stimulation.
authors.affiliation: Graduate School of Environmental Health, Azabu University, 1-17-71 Chuo-ku, Sagamihara, Kanagawa, 252-5201, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Kuribayashi
authors.forename: Tetsuro
authors.initials: T
authors.lastname: Seita
authors.forename: Katsuhito
authors.initials: K
authors.lastname: Kawato
authors.forename: Shunsuke
authors.initials: S
authors.lastname: Yamazaki
authors.forename: Shizuo
authors.initials: S
authors.lastname: Yamamoto
mesh_terms: D000210:Acute-Phase Reaction / Q000097:blood*; D000367:Age Factors; D000818:Animals; D054360:Chemokine CXCL1 / Q000096:biosynthesis / Q000097:blood*; D004195:Disease Models, Animal; D007249:Inflammation / Q000097:blood* / Q000378:metabolism; D015850:Interleukin-6 / Q000096:biosynthesis / Q000097:blood*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D014425:Turpentine; D000511:alpha-Macroglobulins / Q000096:biosynthesis*
chemical_list: D054360:Chemokine CXCL1; C513297:Cxcl1 protein, rat; D015850:Interleukin-6; D000511:alpha-Macroglobulins; D014425:Turpentine