title: SLC41A1 is the only magnesium responsive gene significantly overexpressed in placentas of preeclamptic women.
abstract: OBJECTIVE
  To examine expression profile of magnesium responsive genes (MRGs) in placentas of normoevolutive and preeclamptic women.
  
  
  METHODS
  The expression profiles of MRGs were determined in placentas of normoevolutive (N=26) and preeclamptic (N=25) women by RT-qPCR.
  
  
  RESULTS
  Among all tested MRGs (9) only SLC41A1 (encoding for Na(+)/Mg(2+) exchanger) was significantly overexpressed in ~54.2% of preeclamptic (n=24) and in ~9.5% of normoevolutive (n=21) specimens. On average, SLC41A1 was overexpressed sixfold in the preeclamptic group. Presence of SLC41A1 in placentas was confirmed by Western blot analysis. CONCLUSION. SLC41A1 is significantly overexpressed in nearly 55% of preeclamptic placentas. This may indicate a direct contribution of changed Mg homeostasis in the development of preeclampsia.
authors.affiliation: Department Molecular Physiology, Institute of Veterinary-Physiology, Free University of Berlin , Germany .
authors.forename: Martin
authors.initials: M
authors.lastname: Kolisek
authors.forename: Carlos
authors.initials: C
authors.lastname: Galaviz-Hernández
authors.forename: Fernando
authors.initials: F
authors.lastname: Vázquez-Alaniz
authors.forename: Gerhard
authors.initials: G
authors.lastname: Sponder
authors.forename: Saba
authors.initials: S
authors.lastname: Javaid
authors.forename: Katharina
authors.initials: K
authors.lastname: Kurth
authors.forename: Axel
authors.initials: A
authors.lastname: Nestler
authors.forename: Martha
authors.initials: M
authors.lastname: Rodríguez-Moran
authors.forename: Stefan
authors.initials: S
authors.lastname: Verlohren
authors.forename: Fernando
authors.initials: F
authors.lastname: Guerrero-Romero
authors.forename: Jörg Rudolf
authors.initials: JR
authors.lastname: Aschenbach
authors.forename: Jürgen
authors.initials: J
authors.lastname: Vormann
mesh_terms: D000293:Adolescent; D000328:Adult; D016022:Case-Control Studies; D027682:Cation Transport Proteins / Q000378:metabolism*; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D008274:Magnesium / Q000378:metabolism*; D010920:Placenta / Q000378:metabolism*; D011225:Pre-Eclampsia / Q000378:metabolism*; D011247:Pregnancy; D055815:Young Adult
chemical_list: D027682:Cation Transport Proteins; C476304:SLC41A1 protein, human; D008274:Magnesium