title: A new Ki-67 / E-cadherin cocktail reduces inter-observer variation of the calculated proliferative index.
abstract: The proliferative index in breast carcinoma is usually calculated by the percentage of the Ki-67 positive cells out of the total number of malignant cells. In order to reduce the inter-observer variability of the calculated proliferative index a cocktail of antibodies against E-Cadherin and Ki-67 (Ki/Cad Cocktail) is presented. The cocktail was applied on 59 cases of infiltrating duct carcinoma of breast and compared to the consecutive slides stained for Ki-67 alone. The Ki/Cad cocktail has the advantage that by adding the anti E-Cadherin antibody, all the malignant epithelial cells are highlighted and can be differentiated from other proliferating cells. Statistical analysis proved that the cocktail increases the inter-observer agreement from 89 % to 97 % as compared to the Ki-67 alone and also reduces the overlap between the cancer grades.
authors.affiliation: Department of Biology, Hadassah University, Jerusalem, Israel.
authors.forename: Golan
authors.initials: G
authors.lastname: Bubis
authors.forename: Ohad
authors.initials: O
authors.lastname: Hilly
authors.forename: Roy
authors.initials: R
authors.lastname: Bubis
authors.forename: Marisa
authors.initials: M
authors.lastname: Halpern
authors.forename: Ariel
authors.initials: A
authors.lastname: Schwartz
authors.forename: Rumelia
authors.initials: R
authors.lastname: Koren
authors.forename: Lea
authors.initials: L
authors.lastname: Rath-Wolfson
mesh_terms: D001943:Breast Neoplasms / Q000737:chemistry* / Q000378:metabolism / Q000473:pathology; D015820:Cadherins / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D018270:Carcinoma, Ductal, Breast / Q000737:chemistry* / Q000378:metabolism / Q000473:pathology; D048708:Cell Growth Processes / Q000502:physiology; D004396:Coloring Agents / Q000737:chemistry*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods / Q000592:standards; D019394:Ki-67 Antigen / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D060787:Neoplasm Grading; D015588:Observer Variation
chemical_list: D015820:Cadherins; D004396:Coloring Agents; D019394:Ki-67 Antigen