title: Proteomic analysis of Trichinella spiralis muscle larval excretory-secretory proteins recognized by early infection sera.
abstract: Although the excretory-secretory (ES) proteins of Trichinella spiralis muscle larvae are the most commonly used diagnostic antigens for trichinellosis, their main disadvantage is the false negative results during the early stage of infection and cross-reaction of their main components (43, 45, 49, and 53 kDa) with sera of patients with other helminthiasis. The aim of this study was to identify early specific diagnostic antigens in T. spiralis ES proteins with 30-40 kDa. The ES proteins were analyzed by two-dimensional electrophoresis (2-DE), and a total of approximately 150 proteins spots were detected with isoelectric point (pI) varying from 4 to 7 and molecular weight from 14 to 66 kDa. When probed with sera from infected mice at 18 days postinfection, ten protein spots with molecular weight of 30-40 kDa were recognized and identified by MALDI-TOF/TOF-MS. All of ten spots were successfully identified and characterized to correlate with five different proteins, including two potential serine proteases, one antigen targeted by protective antibodies, one deoxyribonuclease (DNase) II, and one conserved hypothetical protein. These proteins might be the early specific diagnostic antigens for trichinellosis.
authors.affiliation: Department of Parasitology, Medical College, Zhengzhou University, Zhengzhou 450052, China.
authors.forename: Li
authors.initials: L
authors.lastname: Wang
authors.forename: Zhong Quan
authors.initials: ZQ
authors.lastname: Wang
authors.forename: Dan Dan
authors.initials: DD
authors.lastname: Hu
authors.forename: Jing
authors.initials: J
authors.lastname: Cui
mesh_terms: D000818:Animals; D000947:Antigens, Helminth / Q000378:metabolism*; D015153:Blotting, Western; D015180:Electrophoresis, Gel, Two-Dimensional; D063990:Gene Ontology; D015801:Helminth Proteins / Q000378:metabolism*; D007814:Larva / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D009132:Muscles / Q000469:parasitology*; D040901:Proteomics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D034421:Sus scrofa; D017160:Trichinella spiralis / Q000378:metabolism*; D014235:Trichinellosis / Q000097:blood* / Q000378:metabolism / Q000469:parasitology*
chemical_list: D000947:Antigens, Helminth; D015801:Helminth Proteins; C097419:excretory-secretory antigen, Trichinella