title: Nationwide study of Escherichia coli producing extended-spectrum β-lactamases TEM, SHV and CTX-M in Turkey.
abstract: Four hundred and forty extended-spectrum β-lactamase (ESBL)-producing Escherichia coli isolates were collected from 10 different hospitals in Turkey between 2011 and 2012. Clinical specimens consisted of urine (80.45%), blood (6.59%), cerebrospinal fluid (1.13%), pleural fluid (2.95%), wound (4.31%) and sputum (4.54%). ESBL-coding genes (CTX-M1, CTX-M2, TEM, SHV) were detected by PCR. According to the PCR and sequencing results, CTX-M1 was the most prevalent β-lactamase 83.18% (366/440), followed by TEM 44.09% (194/440), CTX-M2 31.81% (140/440) and SHV 1.81% (8/440). Sequencing results showed that TEM and SHV types were TEM-1b and SHV-11, respectively. Rate of the strains harboring only CTX-M1, CTX-M2, TEM-1b and SHV-11 were 30.90%, 3.63%, 2.27% and 0.23%, respectively. Rate of the strains harboring the combinations of CTX-M1-CTX-M2, CTX-M1-CTX-M2-TEM-1b, CTX-M2-TEM-1b, CTX-M1-TEM-1b, CTX-M1-CTX-M2-TEM-1b-SHV-11, CTX-M1-TEM-1b-SHV-11, CTX-M1-SHV-11, CTX-M1-CTX-M2-SHV-11, CTX-M2-SHV-11, CTX-M2-TEM-1b-SHV-11, TEM-1b-SHV-11 were 12.95%, 11.59%, 2.95%, 26.13%, 0.45%, 0.68%, 0.22%, 0.22%, 0%, 0% and 0%, respectively. This is a nationwide study of ESBL-producing E. coli in Turkey. These results shows that CTX-M1 group is the most common type of class A β-lactamases among ESBL-producing E. coli strains in Turkey.
authors.affiliation: Department of Medical Microbiology, Faculty of Medicine, Recep Tayyip Erdogan University, Rize, Turkey.
authors.forename: Aysegul
authors.initials: A
authors.lastname: Copur Cicek
authors.forename: Aysegul
authors.initials: A
authors.lastname: Saral
authors.forename: Azer
authors.initials: A
authors.lastname: Ozad Duzgun
authors.forename: Ekrem
authors.initials: E
authors.lastname: Yasar
authors.forename: Zeynep
authors.initials: Z
authors.lastname: Cizmeci
authors.forename: Pervin
authors.initials: P
authors.lastname: Ozlem Balci
authors.forename: Fatma
authors.initials: F
authors.lastname: Sari
authors.forename: Mehmet
authors.initials: M
authors.lastname: Firat
authors.forename: Yasemin Ay
authors.initials: YA
authors.lastname: Altintop
authors.forename: Sibel
authors.initials: S
authors.lastname: Ak
authors.forename: Ahmet
authors.initials: A
authors.lastname: Caliskan
authors.forename: Nazan
authors.initials: N
authors.lastname: Yildiz
authors.forename: Metin
authors.initials: M
authors.lastname: Sancaktar
authors.forename: Emine
authors.initials: E
authors.lastname: Esra Budak
authors.forename: Ayse
authors.initials: A
authors.lastname: Erturk
authors.forename: Osman
authors.initials: O
authors.lastname: Birol Ozgumus
authors.forename: Cemal
authors.initials: C
authors.lastname: Sandalli
mesh_terms: D004269:DNA, Bacterial / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics* / Q000302:isolation & purification; D004927:Escherichia coli Infections / Q000382:microbiology*; D006801:Humans; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D014421:Turkey; D001618:beta-Lactamases / Q000235:genetics*
chemical_list: D004269:DNA, Bacterial; C071167:beta-lactamase CTX-2; C107197:beta-lactamase SHV-11; C055184:beta-lactamase TEM-3; D001618:beta-Lactamases; C034413:beta-lactamase TEM-1