title: Anti-thrombotic activity of DT-13, a saponin isolated from the root tuber of Liriope muscari.
abstract: OBJECTIVE
  To investigate the anti-thrombotic activity of DT-13 in experimental animal models.
  
  
  METHODS
  The anti-thrombotic activity of DT-13 was evaluated by measuring the thrombus induced by inferior vena cava (IVC) ligation in mice and rats. The anti-thrombotic mechanism of DT-13 was investigated by assessing the mRNA expression levels of interleukin-6 (IL-6) and tissue factor (TF) in rat IVC tissue around thrombus.
  
  
  RESULTS
  DT-13 markedly inhibited thrombosis induced by IVC ligation for 6 h in mice (2.0 and 4.0 mg/kg, p.o.) and for 18 h in rats (1.4 mg/kg, p.o.). Furthermore, DT-13 down-regulated the increased mRNA expression levels of IL-6 and TF in rats.
  
  
  CONCLUSIONS
  DT-13 has an anti-thrombotic activity due to down-regulation of the increased mRNA expression levels of IL-6 and TF.
authors.affiliation: Department of Complex Prescription of TCM, China Pharmaceutical University, Nanjing, China.
authors.forename: Youqing
authors.initials: Y
authors.lastname: Tian
authors.forename: Shengtang
authors.initials: S
authors.lastname: Ma
authors.forename: Biqi
authors.initials: B
authors.lastname: Lin
authors.forename: Junping
authors.initials: J
authors.lastname: Kou
authors.forename: Boyang
authors.initials: B
authors.lastname: Yu
mesh_terms: D000818:Animals; D000991:Antithrombins / Q000494:pharmacology / Q000627:therapeutic use*; D015850:Interleukin-6 / Q000235:genetics; D031424:Liriope Plant; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D008517:Phytotherapy; D035281:Plant Tubers; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D012503:Saponins / Q000494:pharmacology / Q000627:therapeutic use*; D013925:Thromboplastin / Q000235:genetics; D014682:Vena Cava, Inferior; D020246:Venous Thrombosis / Q000188:drug therapy* / Q000378:metabolism
chemical_list: D000991:Antithrombins; D015850:Interleukin-6; D012333:RNA, Messenger; D012503:Saponins; C578958:ruscogenin-1-O-(glucopyranosyl-(1-2))(xylopyranosyl-(1-3))fucopyranoside; D013925:Thromboplastin
keywords: Anti-thrombotic activity; DT-13; interleukin-6; radix Liriope muscari; tissue factor