title: Laccase complex with polyvinylamine bearing grafted TEMPO is a cellulose adhesion primer.
abstract: Polyelectrolyte complexes formed between laccase and polyvinylamine with grafted TEMPO moieties, PVAm-T, adsorb onto cellulose, causing oxidation. All evidence supports the view that aldehyde groups on oxidized cellulose condense with primary amine groups, giving a grafted layer of PVAm-T complexed with laccase. The grafted PVAm-T serves as a primer layer promoting wet cellulose-to-cellulose adhesion in the presence of PVAm adhesive. The cellulose modification occurs at ambient temperatures and pH 5. The adhesion improvements with mixtures of PVAm-T and laccase are remarkable because both components are macromolecular, which should inhibit the ability of the TEMPO to act as a shuttle between the enzyme and the primary hydroxyl groups on cellulose. It is proposed that PVAm-bound oxoammonium ions exchange with neighboring TEMPO moieties, providing a mechanism for the transfer of oxidation activity from immobilized enzyme to the cellulose surfaces.
authors.affiliation: Department of Chemical Engineering, McMaster University, Hamilton, Ontario, Canada.
authors.forename: Jieyi
authors.initials: J
authors.lastname: Liu
authors.forename: Robert
authors.initials: R
authors.lastname: Pelton
authors.forename: Jaclyn M
authors.initials: JM
authors.lastname: Obermeyer
authors.forename: Anton
authors.initials: A
authors.lastname: Esser
mesh_terms: D000269:Adhesives / Q000737:chemistry; D000327:Adsorption; D002482:Cellulose / Q000737:chemistry*; D003497:Cyclic N-Oxides / Q000737:chemistry*; D005656:Fungal Proteins / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D042845:Laccase / Q000737:chemistry*; D055595:Mechanical Phenomena; D016877:Oxidants / Q000737:chemistry*; D010084:Oxidation-Reduction; D010100:Oxygen / Q000737:chemistry; D011145:Polyvinyls / Q000737:chemistry*; D055454:Trametes / Q000201:enzymology
chemical_list: D000269:Adhesives; D003497:Cyclic N-Oxides; D005656:Fungal Proteins; D016877:Oxidants; D011145:Polyvinyls; C534904:polyvinylamine; D002482:Cellulose; D042845:Laccase; D010100:Oxygen; C003959:TEMPO