title: Litorilituus sediminis gen. nov. sp. nov., isolated from coastal sediment of an amphioxus breeding zone in Qingdao, China.
abstract: A Gram-negative, non-spore-forming, catalase- and oxidase-positive, aerobic, curved-rod shaped bacterium with polar or subpolar flagellum, designated strain JYr2(T), was isolated from a sediment sample collected from an amphioxus breeding zone in the coastal region of Qingdao, China. The organism grew optimally at 37 °C, pH 8-9 and in the presence of 3 % (w/v) NaCl or 3-4 % sea salts (w/v; Sigma). Salt was required for growth. The strain contained isoprenoid quinone 8 (Q-8, 100 %) as the predominant isoprenoid quinone and C16:0 (24.1 %) and C16:1 ω7c and/or C16:1 ω6c (35.8 %) as major fatty acids. Phosphatidylethanolamine, phosphatidylglycerol, aminophospholipid (PN) and two aminolipid (AL1, AL2) were the major constituents of the phospholipids. A phylogenetic analysis based on 16S rRNA gene sequences indicated that strain JYr2(T) formed a distinct evolutionary lineage within the family Colwelliaceae. It showed <95.0 % sequence similarities to all validly published species in the family Colwelliaceae, except 95.2 % to Thalassomonas viridans DSM 13754(T) and 95.0 % to Colwellia polaris JCM 537(T). The G+C content of the DNA was 42.7 mol%. On the basis of the polyphasic taxonomic study, strain JYr2(T) (= CGMCC 1.10794(T) = JCM 17549(T)) was considered to represent a novel genus and species in Gammaproteobacteria, for which the name Litorilituus sediminis gen. nov. sp. nov. was proposed.
authors.affiliation: Department of Life Sciences and Technology, Xinxiang Medical University, 601 Jinsui Avenue, Xinxiang, People's Republic of China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.forename: Rui
authors.initials: R
authors.lastname: Zhao
authors.forename: Shiqi
authors.initials: S
authors.lastname: Ji
authors.forename: Zhao
authors.initials: Z
authors.lastname: Li
authors.forename: Tong
authors.initials: T
authors.lastname: Yu
authors.forename: Bingyu
authors.initials: B
authors.lastname: Li
authors.forename: Xiaochong
authors.initials: X
authors.lastname: Shi
authors.forename: Xiao-Hua
authors.initials: XH
authors.lastname: Zhang
mesh_terms: D000332:Aerobiosis; D000818:Animals; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002374:Catalase / Q000032:analysis; D002681:China; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D005407:Flagella / Q000502:physiology; D020563:Gammaproteobacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D019015:Geologic Sediments / Q000382:microbiology*; D006863:Hydrogen-Ion Concentration; D064175:Lancelets / Q000254:growth & development*; D008969:Molecular Sequence Data; D010088:Oxidoreductases / Q000032:analysis; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D011809:Quinones / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride / Q000378:metabolism; D013696:Temperature
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D010743:Phospholipids; D011809:Quinones; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride; D010088:Oxidoreductases; D002374:Catalase