title: Effect of 1-methyl-D-tryptophan and adoptive transfer of dendritic cells on polymicrobial sepsis induced by cecal content injection.
abstract: A mouse model of polymicrobial sepsis induced by cecal content injection (CCI) was developed with the aim of gaining a better understanding of the mechanism of sepsis. This model has a similar survival pattern to the conventional model with the added benefits of ability to vary the severity of sepsis and greater consistency. Administration of 1-methyl-D-tryptophan (1-MT) to inhibit indoleamine 2,3-dioxygenase (IDO) in mice with CCI-induced sepsis increased the survival rate and tended to up-regulate IL-10/IL-12 serum concentrations. The effectiveness of 1-MT was confirmed by increases in IL-10 over IL-12 in bone marrow-derived dendritic cells (BMDCs) treated with LPS and 1-MT and a superior survival rate 24 hr after injection of these double treated BMDCs in the CCI-induced sepsis model. Therefore, CCI is both a useful and reliable technique for investigating polymicrobial sepsis. The present findings using this newly developed model suggest that inhibition of IDO alleviates the severity of polymicrobial sepsis and modulates the immune response even in cases of severe systemic septic inflammation.
authors.affiliation: Department of Physiology, Korea University College of Medicine.
authors.forename: Hong Soon
authors.initials: HS
authors.lastname: Yim
authors.forename: Kyung Min
authors.initials: KM
authors.lastname: Choi
authors.forename: Byoungjae
authors.initials: B
authors.lastname: Kim
authors.forename: In Duk
authors.initials: ID
authors.lastname: Jung
authors.forename: Yeong-Min
authors.initials: YM
authors.lastname: Park
authors.forename: Yoon Kyu
authors.initials: YK
authors.lastname: Kang
authors.forename: Min-Goo
authors.initials: MG
authors.lastname: Lee
mesh_terms: D019264:Adoptive Transfer; D000818:Animals; D002432:Cecum / Q000737:chemistry / Q000382:microbiology; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000187:drug effects / Q000276:immunology*; D004195:Disease Models, Animal; D006801:Humans; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D018805:Sepsis / Q000276:immunology* / Q000382:microbiology; D014364:Tryptophan / Q000008:administration & dosage / Q000031:analogs & derivatives*
chemical_list: C028129:tryptophan methyl ester; D014364:Tryptophan
keywords: clinical immunology; dendritic cells; innate immunity