title: Functional characterization on invertebrate and vertebrate tissues of tachykinin peptides from octopus venoms.
abstract: It has been previously shown that octopus venoms contain novel tachykinin peptides that despite being isolated from an invertebrate, contain the motifs characteristic of vertebrate tachykinin peptides rather than being more like conventional invertebrate tachykinin peptides. Therefore, in this study we examined the effect of three variants of octopus venom tachykinin peptides on invertebrate and vertebrate tissues. While there were differential potencies between the three peptides, their relative effects were uniquely consistent between invertebrate and vertebrae tissue assays. The most potent form (OCT-TK-III) was not only the most anionically charged but also was the most structurally stable. These results not only reveal that the interaction of tachykinin peptides is more complex than previous structure-function theories envisioned, but also reinforce the fundamental premise that animal venoms are rich resources of novel bioactive molecules, which are useful investigational ligands and some of which may be useful as lead compounds for drug design and development.
authors.affiliation: Venom Evolution Laboratory, School of Biological Sciences, University of Queensland, St Lucia, Queensland 4072, Australia.
authors.forename: Tim
authors.initials: T
authors.lastname: Ruder
authors.forename: Syed Abid
authors.initials: SA
authors.lastname: Ali
authors.forename: Kiel
authors.initials: K
authors.lastname: Ormerod
authors.forename: Andreas
authors.initials: A
authors.lastname: Brust
authors.forename: Mary-Louise
authors.initials: ML
authors.lastname: Roymanchadi
authors.forename: Sabatino
authors.initials: S
authors.lastname: Ventura
authors.forename: Eivind A B
authors.initials: EA
authors.lastname: Undheim
authors.forename: Timothy N W
authors.initials: TN
authors.lastname: Jackson
authors.forename: A Joffre
authors.initials: AJ
authors.lastname: Mercier
authors.forename: Glenn F
authors.initials: GF
authors.lastname: King
authors.forename: Paul F
authors.initials: PF
authors.lastname: Alewood
authors.forename: Bryan G
authors.initials: BG
authors.lastname: Fry
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003400:Astacoidea / Q000187:drug effects / Q000502:physiology; D007082:Ileum / Q000187:drug effects* / Q000502:physiology; D008297:Male; D008969:Molecular Sequence Data; D008978:Mollusk Venoms / Q000737:chemistry*; D009119:Muscle Contraction / Q000187:drug effects*; D009130:Muscle, Smooth / Q000187:drug effects* / Q000502:physiology; D049831:Octopodiformes / Q000737:chemistry; D011485:Protein Binding; D051381:Rats; D018039:Receptors, Tachykinin / Q000737:chemistry / Q000378:metabolism; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D015320:Tachykinins / Q000138:chemical synthesis / Q000494:pharmacology*
chemical_list: D008978:Mollusk Venoms; D018039:Receptors, Tachykinin; D015320:Tachykinins
keywords: Octopus; Peptide; Tachykinin; Venom