title: Determination of uric acid in plasma by LC-MS/MS and its application to an efficacy evaluation of recombinant urate oxidase.
abstract: A liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the determination of uric acid in human plasma was developed and validated. Separation was achieved on a C18 column by the mobile phase of 30% water (containing 0.5% formic acid) and 70% methanol. Quantification was done using a multiple reaction monitoring (MRM) mode to monitor the precursor-to-product ion transitions of m/z 169.1 → m/z 141.1 for uric acid and m/z 171 → m/z 143 for 1,3-(15)N uric acid (IS) at a positive ionization mode. The calibration curve was established over the range of 0.4096 - 100 mg/L, and the correlation coefficient was better than 0.99. The intra-day and inter-day relative standard deviations were less than 5.1%. The accuracy determined at three concentrations ranged between 92.7 and 102.3%. This method was successfully applied to an efficacy study of intravenous recombinant urate oxidase produced by Escherichia coli in a clinical phase I study.
authors.affiliation: Research Institute of Drug Metabolism and Pharmacokinetics, School of Pharmaceutical Sciences, Central South University, Changsha, Hunan, P. R. China.
authors.forename: Xi
authors.initials: X
authors.lastname: Luo
authors.forename: Ningfang
authors.initials: N
authors.lastname: Cai
authors.forename: Zeneng
authors.initials: Z
authors.lastname: Cheng
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D057230:Limit of Detection; D011994:Recombinant Proteins / Q000737:chemistry; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014503:Urate Oxidase / Q000737:chemistry*; D014527:Uric Acid / Q000097:blood*
chemical_list: D011994:Recombinant Proteins; D014527:Uric Acid; D014503:Urate Oxidase