title: Amniotic stem cell transplantation therapy for type 1 diabetes: a case report.
abstract: This case report presents an evaluation of the clinical effects of an allogeneic amniotic cell transplant for the treatment of type 1 diabetes mellitus. A 26-year-old man with type 1 diabetes was treated with stem cells isolated from his neonatal son's amniotic membrane, collected at birth (2 × 10(7) cells). The cells, which expressed high levels of cluster of differentiation (CD) 133 and CD34 as assessed by flow cytometry, were infused into the pancreatic dorsal artery through the left femoral artery. The main study outcome was the change in exogenous insulin requirements, which began to decrease 3 days after transplantation. At 3 months post-transplantation, the patient was insulin independent and remained so for 6.2 months. During a 36-month follow-up, the patient's blood glucose remained under control and insulin treatment was readjusted to a dosage of 8 IU/day. These preliminary data suggest that amniotic membrane stem cell transplantation can improve islet-cell function in response to glucose in vivo, although an alternative explanation (such as a honeymoon period due to reduced glucose toxicity) also has to be considered.
authors.affiliation: Stem Cell Clinical Application Centre, Siping Central Hospital, Siping, Jilin Province, China. ly3641829@163.com
authors.forename: Ying
authors.initials: Y
authors.lastname: Liu
authors.forename: Dong-Lin
authors.initials: DL
authors.lastname: Cao
authors.forename: Li-Bin
authors.initials: LB
authors.lastname: Guo
authors.forename: Sheng-Nan
authors.initials: SN
authors.lastname: Guo
authors.forename: Jin-Kai
authors.initials: JK
authors.lastname: Xu
authors.forename: Hong-Feng
authors.initials: HF
authors.lastname: Zhuang
mesh_terms: D000328:Adult; D000650:Amnion / Q000166:cytology / Q000276:immunology / Q000637:transplantation*; D001786:Blood Glucose / Q000378:metabolism; D002469:Cell Separation; D003922:Diabetes Mellitus, Type 1 / Q000097:blood / Q000628:therapy*; D006801:Humans; D007231:Infant, Newborn; D007275:Injections, Intravenous; D007328:Insulin / Q000096:biosynthesis / Q000097:blood* / Q000627:therapeutic use; D007333:Insulin Resistance / Q000276:immunology*; D008297:Male; D009679:Nuclear Family; D033581:Stem Cell Transplantation / Q000379:methods*; D014184:Transplantation, Homologous
chemical_list: D001786:Blood Glucose; D007328:Insulin
keywords: Type 1 diabetes mellitus; amniotic stem cell transplantation; glycosylated haemoglobin (HbA1c); insulin