title: Sequential determination of fat- and water-soluble vitamins in Rhodiola imbricata root from trans-Himalaya with rapid resolution liquid chromatography/tandem mass spectrometry.
abstract: A rapid method was developed to determine both types of vitamins in Rhodiola imbricata root for the accurate quantification of free vitamin forms. Rapid resolution liquid chromatography/tandem mass spectrometry (RRLC-MS/MS) with electrospray ionization (ESI) source operating in multiple reactions monitoring (MRM) mode was optimized for the sequential analysis of nine water-soluble vitamins (B1, B2, two B3 vitamins, B5, B6, B7, B9, and B12) and six fat-soluble vitamins (A, E, D2, D3, K1, and K2). Both types of vitamins were separated by ion-suppression reversed-phase liquid chromatography with gradient elution within 30 min and detected in positive ion mode. Deviations in the intra- and inter-day precision were always below 0.6% and 0.3% for recoveries and retention time. Intra- and inter-day relative standard deviation (RSD) values of retention time for water- and fat-soluble vitamin were ranged between 0.02-0.20% and 0.01-0.15%, respectively. The mean recoveries were ranged between 88.95 and 107.07%. Sensitivity and specificity of this method allowed the limits of detection (LOD) and limits of quantitation (LOQ) of the analytes at ppb levels. The linear range was achieved for fat- and water-soluble vitamins at 100-1000 ppb and 10-100 ppb. Vitamin B-complex and vitamin E were detected as the principle vitamins in the root of this adaptogen which would be of great interest to develop novel foods from the Indian trans-Himalaya.
authors.affiliation: Defence Institute of High Altitude Research, Defence Research & Development Organisation, Leh-Ladakh, Jammu & Kashmir 194101, India.
authors.forename: Amol B
authors.initials: AB
authors.lastname: Tayade
authors.forename: Priyanka
authors.initials: P
authors.lastname: Dhar
authors.forename: Jatinder
authors.initials: J
authors.lastname: Kumar
authors.forename: Manu
authors.initials: M
authors.lastname: Sharma
authors.forename: Om P
authors.initials: OP
authors.lastname: Chaurasia
authors.forename: Ravi B
authors.initials: RB
authors.lastname: Srivastava
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods*; D005223:Fats / Q000737:chemistry; D007194:India; D057230:Limit of Detection; D018517:Plant Roots / Q000737:chemistry*; D010946:Plants, Medicinal / Q000737:chemistry; D015203:Reproducibility of Results; D029771:Rhodiola / Q000737:chemistry*; D012995:Solubility; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014815:Vitamins / Q000032:analysis* / Q000737:chemistry; D014867:Water
chemical_list: D005223:Fats; D014815:Vitamins; D014867:Water
keywords: Fat-soluble vitamins; Rapid resolution liquid chromatography; Rhodiola imbricata; Sequential determination; Tandem mass spectrometry; Water-soluble vitamins