title: The influence of amifostine administration prior to cyclophosphamide on in vitro maturation of mouse oocytes.
abstract: OBJECTIVE
  The protective effect of amifostine against cyclophosphamide (CP) was evaluated on mouse oocytes.
  
  
  METHODS
  Female mice were divided into four groups as follows: group1: cyclophosphamide (CP) (75 mg/kg, i.p) injection, group2: amifostine (250 mg/kg, i.p) injection, group3: amifostine (250 mg/kg, i.p) administered prior to CP (75 mg/kg, i.p) injection, Control group with injection of saline. About 21 days after injection, in vitro maturation (IVM) of oocytes was recorded. Furthermore the percentage of aneuploid oocytes was determined.
  
  
  RESULTS
  In the CP group IVM rate was significantly decreased and aneploidy rate was significantly increased when compared to other groups (p < 0.05). With the administration of Amifostine prior to CP injection IVM rate was increased and aneploidy rate was decreased.
  
  
  CONCLUSIONS
  Depletion in IVM rate with administration of CP is due its adverse effects on oocyte quality. Amifostine administration prior to CP injection appears to modulate deleterious effects of CP on oocytes.
authors.affiliation: Department of Genetics at Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, No 12, Hafez, Banihashem St. Resalat Ave, Tehran 19295-4644, Iran.
authors.forename: Afsaneh
authors.initials: A
authors.lastname: Golkar-Narenji
authors.forename: Zeinab
authors.initials: Z
authors.lastname: Barekati
authors.forename: Hussein
authors.initials: H
authors.lastname: Eimani
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Shabani
authors.forename: Hamid
authors.initials: H
authors.lastname: Gourabi
mesh_terms: D004999:Amifostine / Q000008:administration & dosage*; D000782:Aneuploidy; D000818:Animals; D002467:Cell Nucleus / Q000187:drug effects / Q000502:physiology; D002875:Chromosomes / Q000187:drug effects; D003520:Cyclophosphamide / Q000008:administration & dosage*; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C; D009153:Mutagens / Q000008:administration & dosage / Q000494:pharmacology; D009865:Oocytes / Q000187:drug effects* / Q000254:growth & development; D011837:Radiation-Protective Agents / Q000008:administration & dosage / Q000494:pharmacology
chemical_list: D009153:Mutagens; D011837:Radiation-Protective Agents; D003520:Cyclophosphamide; D004999:Amifostine