title: Dynamic nuclear polarization enhanced NMR in the solid-state.
abstract: Nuclear magnetic resonance (NMR) spectroscopy is one of the most commonly used spectroscopic techniques to obtain information on the structure and dynamics of biological and chemical materials. A variety of samples can be studied including solutions, crystalline solids, powders and hydrated protein extracts. However, biological NMR spectroscopy is limited to concentrated samples, typically in the millimolar range, due to its intrinsic low sensitivity compared to other techniques such as fluorescence or electron paramagnetic resonance (EPR) spectroscopy.Dynamic nuclear polarization (DNP) is a method that increases the sensitivity of NMR by several orders of magnitude. It exploits a polarization transfer from unpaired electrons to neighboring nuclei which leads to an absolute increase of the signal-to-noise ratio (S/N). Consequently, biological samples with much lower concentrations can now be studied in hours or days compared to several weeks.This chapter will explain the different types of DNP enhanced NMR experiments, focusing primarily on solid-state magic angle spinning (MAS) DNP, its applications, and possible means of improvement.
authors.affiliation: NMR Supported Structural Biology, Leibniz Institute für Molekulare Pharmakologie, Robert Roessle Str. 10, 13125, Berlin, Germany, akbey@fmp-berlin.de.
authors.forename: Umit
authors.initials: U
authors.lastname: Akbey
authors.forename: W Trent
authors.initials: WT
authors.lastname: Franks
authors.forename: Arne
authors.initials: A
authors.lastname: Linden
authors.forename: Marcella
authors.initials: M
authors.lastname: Orwick-Rydmark
authors.forename: Sascha
authors.initials: S
authors.lastname: Lange
authors.forename: Hartmut
authors.initials: H
authors.lastname: Oschkinat
mesh_terms: D009682:Magnetic Resonance Spectroscopy / Q000379:methods*