title: Knockdown of the placental growth factor gene inhibits laser induced choroidal neovascularization in a murine model.
abstract: OBJECTIVE
  To evaluate the effect of placental growth factor (PlGF) gene knockdown in a murine model of laser-induced choroidal neovascularization.
  
  
  METHODS
  Choroidal neovascularization was induced in the left eyes of 11 mice by infrared laser. Small interfering RNA (siRNA, 20 picomoles/10 μl) corresponding to PlGF mRNA was administered intravitreally by Hamilton syringe in all subjects. One month later, fluorescein angiography and histolologic examination were performed.
  
  
  RESULTS
  No leakage was apparent in the 11 eyes treated with siRNA cognate to PlGF. The results of histological evaluation were consistent with angiographic findings showing absence of choroidal neovascularization.
  
  
  CONCLUSIONS
  Knockdown of the PlGF gene can inhibit the growth of laser-induced choroidal neovascularization in mice.
authors.affiliation: Ophthalmic Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors.forename: Ramin
authors.initials: R
authors.lastname: Nourinia
authors.forename: Zahra-Soheila
authors.initials: ZS
authors.lastname: Soheili
authors.forename: Hamid
authors.initials: H
authors.lastname: Ahmadieh
authors.forename: Hassan
authors.initials: H
authors.lastname: Akrami
authors.forename: Mozhgan
authors.initials: M
authors.lastname: Rezaei Kanavi
authors.forename: Shahram
authors.initials: S
authors.lastname: Samiei
keywords: Choroidal Neovascularization; Placental Growth Factor; Small Interfering RNA