title: DNA barcodes and molecular diagnostics for distinguishing introduced Xyleborus (Coleoptera: Scolytinae) species in China.
abstract: Members of the large and complex genus Xyleborus (Coleoptera: Scolytinae: Xyleborini) are the most commonly intercepted beetles associated with solid wood-packing materials at ports of entry in China. The accurate identification of species is critical in preventing the invasion of exotic insects. Considering the difficulties in morphological identification, genetic divergences of mitochondrial cytochrome c oxidase subunit I (COI) genes have been used in insect species identification. In this study, 32 Xyleborus and 2 outgroup species were collected from Jiangsu ports and selected to evaluate the effectiveness of DNA barcoding for Xyleborus species. The results showed that the mean interspecific divergence values (23.6%) were 15-fold higher than the observed intraspecific divergence (1.6%), except Xyleborus affinis. The results supported the inference that the barcode variation within species of insects is somewhat higher than interspecific ones. Thus, this study validated the effectiveness of barcoding for the identification of Xyleborus species.
authors.affiliation: College of Forest Resources and Environment, Nanjing Forestry university , Nanjing, Jiangsu Province , P.R. China .
authors.forename: Hong
authors.initials: H
authors.lastname: Chang
authors.forename: Qun
authors.initials: Q
authors.lastname: Liu
authors.forename: Dejun
authors.initials: D
authors.lastname: Hao
authors.forename: Yong
authors.initials: Y
authors.lastname: Liu
authors.forename: Yulin
authors.initials: Y
authors.lastname: An
authors.forename: Lu
authors.initials: L
authors.lastname: Qian
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Yang
mesh_terms: D000818:Animals; D001483:Base Sequence; D002681:China; D001517:Coleoptera / Q000235:genetics*; D003132:Commerce / Q000295:instrumentation; D019295:Computational Biology; D058893:DNA Barcoding, Taxonomic / Q000379:methods*; D017931:DNA Primers / Q000235:genetics; D004272:DNA, Mitochondrial / Q000235:genetics*; D003576:Electron Transport Complex IV / Q000235:genetics*; D014644:Genetic Variation; D058865:Introduced Species; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA; D014934:Wood / Q000469:parasitology*
chemical_list: D017931:DNA Primers; D004272:DNA, Mitochondrial; D003576:Electron Transport Complex IV