title: Purification, crystallization and preliminary X-ray crystallographic study of the tRNA pseudouridine synthase TruB from Streptococcus pneumoniae.
abstract: The RNA pseudouridine synthase TruB catalyses the isomerization of uridine to pseudouridine (Ψ) at residue 55 of elongator tRNAs. In order to better elucidate the functions of TruB in the formation of pseudouridine, the three-dimensional structure of full-length TruB was determined by X-ray crystallography. Here, the expression, purification, crystallization and preliminary crystallographic analysis of TruB from Streptococcus pneumoniae are reported. The crystal belonged to space group P2, with unit-cell parameters a = 37.65, b = 78.09, c = 56.33 Å, β = 102.05°, and diffracted to a resolution of 1.7 Å. The crystal is most likely to contain one molecule in the asymmetric unit, with a VM value of 2.40 Å(3) Da(-1).
authors.affiliation: Key Laboratory of Molecular Biology on Infectious Disease, Chongqing Medical University, Chongqing 400016, People's Republic of China.
authors.forename: Wei
authors.initials: W
authors.lastname: Yang
authors.forename: Shasha
authors.initials: S
authors.lastname: Zhao
authors.forename: Li
authors.initials: L
authors.lastname: Jin
authors.forename: Zhen
authors.initials: Z
authors.lastname: Guo
authors.forename: Shaocheng
authors.initials: S
authors.lastname: Zhang
authors.forename: Hongpeng
authors.initials: H
authors.lastname: Zhang
authors.forename: Deqiang
authors.initials: D
authors.lastname: Wang
mesh_terms: D003460:Crystallization; D018360:Crystallography, X-Ray; D019751:Intramolecular Transferases / Q000737:chemistry* / Q000302:isolation & purification* / Q000378:metabolism; D012343:RNA, Transfer / Q000378:metabolism*; D013296:Streptococcus pneumoniae / Q000201:enzymology*
chemical_list: D012343:RNA, Transfer; D019751:Intramolecular Transferases; C023199:tRNA-pseudouridine synthase I
keywords: Streptococcus pneumoniae; TruB