title: Expression of the calcium binding proteins Necab-1,-2 and -3 in the adult mouse hippocampus and dentate gyrus.
abstract: The family of EF-hand calcium binding proteins is composed of more than 250 members. In search for other neuronal markers, we studied the expression pattern of Necab-1, -2 and -3 in the Ammons horn of adult mice at the gene- and protein levels using in-situ hybridization and immunohistochemistry. The genes for the three Necab's were expressed in specific, non-overlapping areas of the hippocampus. A minority of the Necab-positive interneurons were GABA-ergic, and they virtually never coexpressed one of the classical calcium binding proteins (calretinin, calbindin D-28k and parvalbumin). Necab's are promising new neuronal markers in the brain.
authors.affiliation: Division of Anatomy and Program in Neuroscience, University of Fribourg, Rte. A.Gockel 1, CH-1700 Fribourg, Switzerland.
authors.forename: B
authors.initials: B
authors.lastname: Zimmermann
authors.forename: F
authors.initials: F
authors.lastname: Girard
authors.forename: Z
authors.initials: Z
authors.lastname: Mészàr
authors.forename: M R
authors.initials: MR
authors.lastname: Celio
mesh_terms: D000818:Animals; D002135:Calcium-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D018891:Dentate Gyrus / Q000378:metabolism*; D005136:Eye Proteins / Q000235:genetics / Q000378:metabolism*; D005260:Female; D015870:Gene Expression; D006624:Hippocampus / Q000378:metabolism*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL
chemical_list: D002135:Calcium-Binding Proteins; D005136:Eye Proteins; C438750:NECAB2 protein, mouse; C485870:Necab3 protein, mouse
keywords: ACB; AD; CBX; CM; CP; CTX; Calbindin; Calcium binding-proteins; Calretinin; DR; Diverse: AON; GABA-cells; Gr; HPF; HYP; IO; Interneurons; LP/LD; LRN; LS; MARN; MB; MD/IMD; MEV; MM; MY; OB; OT; P; PB; PG; PIR; PRN; PSV; PVT; Parvalbumin; Pu; Purkinje layer; RH; RN; SC; SN; SPV; TH; TRN; TRS; TT; V; VII; VN; VTA; X; XII; anterior olfactory nucleus; anterodorsal nucleus of the thalamus; caudoputamen; central medial nucleus of the thalamus; cerebellar cortex; cortex; dorsal nucleus raphe; expressed; facial motor nucleus; gl; glomerular layer; gr; granular layer; hippocampal formation; hypoglossal nucleus; hypothalamus; inferior olivary complex; isl; islands of Calleja; lateral posterior/dorsal nucleus of the thalamus; lateral reticular nucleus; lateral septal nucleus; magnocellular reticular nucleus.; medial mammillary nucleus; mediodorsal/intermediodorsal nucleus of the thalamus; medulla; midbrain; midbrain trigeminal nucleus; motor nucleus of trigeminal; ne; not expressed; nucleus accumbens; olfactory bulb; olfactory tubercle; parabrachial nucleus; paraventricular nucleus of the thalamus; piriform area; pons; pontine gray; pontine reticular nucleus; principal sensory nucleus of the trigeminal; red nucleus; rhomboid nucleus; spinal nucleus of the trigeminal; substantia nigra; superior colliculus; tegmental reticular nucleus; tenia tecta (dorsal and ventral parts); thalamus; triangular nucleus of septum; ventral nucleus of the thalamus; ventral tegmental area