title: Simultaneous electrochemical detection of ascorbic acid, dopamine and uric acid based on graphene anchored with Pd-Pt nanoparticles.
abstract: Pd-Pt bimetallic nanoparticles anchored on functionalized reduced graphene oxide (RGO) nanomaterials were synthesized via a one-step in situ reduction process, in which Pt and Pd ions were first attached to poly(diallyldimethylammonium chloride) (PDDA) functionalized graphene oxide (GO) sheets, and then the encased metal ions and GO were subjected to simultaneous reduction by ethylene glycol. The as-prepared Pd3Pt1/PDDA-RGO nanocomposites were characterized by transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS), Raman spectroscopy and electrochemical methods. In addition, an electrochemical sensor based on the graphene nanocomposites was fabricated for the simultaneous detection of ascorbic acid (AA), dopamine (DA) and uric acid (UA) in their ternary mixture. Three well-separated voltammetric peaks along with remarkable increasing electro-oxidation currents were obtained in differential pulse voltammetry (DPV) measurements. Under the optimized conditions, there were linear relationships between the peak currents and the concentrations in the range of 40-1200 μM for AA, 4-200 μM for DA and 4-400 μM for UA, with the limit of detection (LOD) (based on S/N=3) of 0.61, 0.04 and 0.10 μM for AA, DA and UA, respectively. This improved electrochemical performance can be attributed to the synergistic effect of metallic nanoparticles and RGO and the combination of the bimetallic nanoparticles. Furthermore, the practical electroanalytical utility of the sensor was demonstrated by the determination of AA, DA and together with UA in human urine and blood serum samples with satisfactory results.
authors.affiliation: School of Pharmacy, Nanjing Medical University, Nanjing 211166, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Yan
authors.affiliation: School of Pharmacy, Nanjing Medical University, Nanjing 211166, China.
authors.forename: Shi
authors.initials: S
authors.lastname: Liu
authors.affiliation: School of Pharmacy, Nanjing Medical University, Nanjing 211166, China.
authors.forename: Zhenqin
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: School of Pharmacy, Nanjing Medical University, Nanjing 211166, China.
authors.forename: Guangwu
authors.initials: G
authors.lastname: He
authors.affiliation: School of Pharmacy, Nanjing Medical University, Nanjing 211166, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Zhou
authors.affiliation: School of Pharmacy, Nanjing Medical University, Nanjing 211166, China.
authors.forename: Haiying
authors.initials: H
authors.lastname: Liang
authors.affiliation: School of Pharmacy, Nanjing Medical University, Nanjing 211166, China.
authors.forename: Lulu
authors.initials: L
authors.lastname: Tian
authors.affiliation: School of Pharmacy, Nanjing Medical University, Nanjing 211166, China.
authors.forename: Xuemin
authors.initials: X
authors.lastname: Zhou
authors.affiliation: School of Pharmacy, Nanjing Medical University, Nanjing 211166, China. Electronic address: huijun_jiang2006@hotmail.com.
authors.forename: Huijun
authors.initials: H
authors.lastname: Jiang
mesh_terms: D001205:Ascorbic Acid / Q000032:analysis* / Q000097:blood / Q000652:urine; D004298:Dopamine / Q000032:analysis* / Q000097:blood / Q000652:urine; D055664:Electrochemical Techniques / Q000379:methods*; D004566:Electrodes; D005898:Glass / Q000737:chemistry; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010084:Oxidation-Reduction; D010165:Palladium / Q000737:chemistry*; D056951:Photoelectron Spectroscopy; D010984:Platinum / Q000737:chemistry*; D011095:Polyethylenes / Q000737:chemistry; D000644:Quaternary Ammonium Compounds / Q000737:chemistry; D012996:Solutions; D013059:Spectrum Analysis, Raman; D014527:Uric Acid / Q000032:analysis* / Q000097:blood / Q000652:urine
chemical_list: D011095:Polyethylenes; D000644:Quaternary Ammonium Compounds; D012996:Solutions; C041004:poly-N,N-dimethyl-N,N-diallylammonium chloride; D014527:Uric Acid; D010984:Platinum; D010165:Palladium; D006108:Graphite; D001205:Ascorbic Acid; D004298:Dopamine
keywords: Ascorbic acid; Dopamine; Graphene; Pd–Pt bimetallic nanoparticles; Simultaneous detection; Uric acid