title: Rapid method for determination of anthocyanin glucosides and free delphinidin in grapes using u-HPLC.
abstract: A rapid method for the determination of anthocyanin glucosides and free delphinidin in food using ultra-high-performance liquid chromatography (u-HPLC) was validated through tests of precision, accuracy and linearity. The u-HPLC separation was performed on a reversed-phase C18 column (particle size 2 µm, i.d. 2 mm, length 100 mm) with a photodiode array detector. The limits of detection and quantification of the u-HPLC analyses ranged from 0.07 to 0.65 mg/kg for nine types of anthocyanin glucosides and from 0.08 to 0.26 mg/kg for delphinidin aglycone. The intra-day and inter-day precision of individual anthocyanin glucosides and delphinidin aglycone were less than 9.42%. All calibration curves showed good linearity (coefficient of determination = 0.99) within the tested ranges. The rapid and simultaneous u-HPLC method presented in this study significantly improved the speed, sensitivity and resolution of the analyses of nine types of anthocyanin glucosides and free delphidinin aglycone in grapes.
authors.affiliation: Food Analysis Center, Korea Food Research Institute, 516, Baekhyeon, Bundang, Seongnam, Gyeonggi, 463-746, Republic of Korea College of Life Sciences and Biotechnology, Korea University, Anam-Dong, Seongbuk-gu, Seoul 136-701, Republic of Korea.
authors.forename: You-Shin
authors.initials: YS
authors.lastname: Shim
authors.affiliation: Food Analysis Center, Korea Food Research Institute, 516, Baekhyeon, Bundang, Seongnam, Gyeonggi, 463-746, Republic of Korea.
authors.forename: Seunghee
authors.initials: S
authors.lastname: Kim
authors.affiliation: Food Analysis Center, Korea Food Research Institute, 516, Baekhyeon, Bundang, Seongnam, Gyeonggi, 463-746, Republic of Korea.
authors.forename: Dongwon
authors.initials: D
authors.lastname: Seo
authors.affiliation: College of Life Sciences and Biotechnology, Korea University, Anam-Dong, Seongbuk-gu, Seoul 136-701, Republic of Korea.
authors.forename: Hyun-Jin
authors.initials: HJ
authors.lastname: Park
authors.affiliation: Food Analysis Center, Korea Food Research Institute, 516, Baekhyeon, Bundang, Seongnam, Gyeonggi, 463-746, Republic of Korea jhkfri@kfri.re.kr.
authors.forename: Jaeho
authors.initials: J
authors.lastname: Ha
mesh_terms: D000872:Anthocyanins / Q000032:analysis* / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D057230:Limit of Detection; D016014:Linear Models; D010936:Plant Extracts / Q000737:chemistry; D015203:Reproducibility of Results; D027843:Vitis / Q000737:chemistry*; D014920:Wine / Q000032:analysis
chemical_list: D000872:Anthocyanins; D010936:Plant Extracts; C017185:delphinidin