title: Association of polymorphisms in solute carrier family 27, isoform A6 (SLC27A6) and fatty acid-binding protein-3 and fatty acid-binding protein-4 (FABP3 and FABP4) with fatty acid composition of bovine milk.
abstract: The main goal of this study was to develop tools for genetic selection of animals producing milk with a lower concentration of saturated fatty acids (SFA) and a higher concentration of unsaturated fatty acids (UFA). The reasons for changing milk fatty acid (FA) composition were to improve milk technological properties, such as for production of more spreadable butter, and milk nutritional value with respect to the potentially adverse effects of SFA on human health. We hypothesized that genetic polymorphisms in solute carrier family 27, isoform A6 (SLC27A6) fatty acid transport protein gene and fatty acid binding protein (FABP)-3 and FABP-4 (FABP3 and FABP4) would affect the selectivity of FA uptake into, and FA redistribution inside, mammary epithelial cells, resulting in altered FA composition of bovine milk. The objectives of our study were to discover genetic polymorphisms in SLC27A6, FABP3, and FABP4, and to test those polymorphisms for associations with milk FA composition. The results showed that after pairwise comparisons between SLC27A6 haplotypes for significantly associated traits, haplotype H3 was significantly associated with 1.37 weight percentage (wt%) lower SFA concentration, 0.091 lower SFA:UFA ratio, and 0.17 wt% lower lauric acid (12:0) concentration, but 1.37 wt% higher UFA and 1.24 wt% higher monounsaturated fatty acid (MUFA) concentrations compared with haplotype H1 during the first 3 mo of lactation. Pairwise comparisons between FABP4 haplotypes for significantly associated traits showed that haplotype H3 was significantly associated with 1.04 wt% lower SFA concentration, 0.079 lower SFA:UFA ratio, 0.15 wt% lower lauric acid (12:0), and 0.27 wt% lower myristic acid (14:0) concentrations, but 1.04 wt% higher UFA and 0.91 wt% higher MUFA concentrations compared with haplotype H1 during the first 3 mo of lactation. Percentages of genetic variance explained by H3 versus H1 haplotype substitutions for SLC27A6 and FABP4 ranged from 2.50 to 4.86% and from 4.91 to 7.22%, respectively. Tag single nucleotide polymorphisms were identified to distinguish haplotypes H3 of SLC27A6 and FABP4 from others encompassing each gene. We found no significant associations between FABP3 haplotypes and milk FA composition. In conclusion, polymorphisms in FABP4 and SLC27A6 can be used to select for cattle producing milk with lower concentrations of SFA and higher concentrations of UFA.
authors.affiliation: Department of Animal Science, Iowa State University, Ames, Iowa 50011, USA.
authors.forename: R A
authors.initials: RA
authors.lastname: Nafikov
authors.forename: J P
authors.initials: JP
authors.lastname: Schoonmaker
authors.forename: K T
authors.initials: KT
authors.lastname: Korn
authors.forename: K
authors.initials: K
authors.lastname: Noack
authors.forename: D J
authors.initials: DJ
authors.lastname: Garrick
authors.forename: K J
authors.initials: KJ
authors.lastname: Koehler
authors.forename: J
authors.initials: J
authors.lastname: Minick-Bormann
authors.forename: J M
authors.initials: JM
authors.lastname: Reecy
authors.forename: D E
authors.initials: DE
authors.lastname: Spurlock
authors.forename: D C
authors.initials: DC
authors.lastname: Beitz
mesh_terms: D000818:Animals; D002417:Cattle / Q000235:genetics*; D050612:Fatty Acid Transport Proteins / Q000235:genetics*; D050556:Fatty Acid-Binding Proteins / Q000235:genetics*; D005227:Fatty Acids / Q000032:analysis*; D005231:Fatty Acids, Unsaturated / Q000032:analysis; D005260:Female; D005838:Genotype; D006239:Haplotypes / Q000235:genetics; D008297:Male; D008892:Milk / Q000737:chemistry*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D020033:Protein Isoforms / Q000235:genetics; D019655:Quantitative Trait, Heritable; D016415:Sequence Alignment / Q000662:veterinary
chemical_list: D050612:Fatty Acid Transport Proteins; D050556:Fatty Acid-Binding Proteins; D005227:Fatty Acids; D005231:Fatty Acids, Unsaturated; D020033:Protein Isoforms
keywords: candidate gene; dairy cow; fatty acid transport; milk fatty acid