title: Sensibility of male rats fertility against olive oil, Nigella sativa oil and pomegranate extract.
abstract: OBJECTIVE
  To clarify the modulatory effects of daily consumption of pomegranate extract (PE), olive oil (OO) and Nagilla sativa oil (NSO) on antioxidant activity, sperm quality and pituitary-testicular axis of adult male wistar rats.
  
  
  METHODS
  Thirty-two adult male Wistar rats were divided into four equal groups, eight rats each. Using rat gastric tubes, 1.0 mL distilled water, 1.0 mL PE, 0.4 mL NSO and 0.4 mL OO were orally administered daily for 6 weeks in the first, second, third and fourth groups, respectively. Reproductive organs, body weight, sperm criteria, testosterone, FSH, LH, inhibin-B, lipid peroxidation, and antioxidant enzyme activities were investigated. At the end of the study protocol, analyses occurred at the same time. Data were analysed by ANOVA test and P<0.05 was considered to be a significant value.
  
  
  RESULTS
  In all studied groups, malondialdehyde level was significantly decreased accompanied with an increases in glutathione peroxidase and glutathione. Rats treated with PE showed an increase in catalase activities accompanied with an increase in sperm concentration which was also observed in NSO group. In PE treated group, sperm motility was also increased accompanied with decreased abnormal sperm rate. NSO, OO and PE treated groups shows an insignificant effect on testosterone, inhibin-B, FSH and LH in comparison with control group.
  
  
  CONCLUSIONS
  These results show that administration of PE, NSO and OO could modify sperm characteristics and antioxidant activity of adult male wistar rats.
authors.affiliation: Biomedical Sciences Department, College of Clinical Pharmacy, King Faisal University, KSA.
authors.forename: Sherif W
authors.initials: SW
authors.lastname: Mansour
authors.forename: Sibghatullah
authors.initials: S
authors.lastname: Sangi
authors.forename: Sree
authors.initials: S
authors.lastname: Harsha
authors.forename: Mueen A
authors.initials: MA
authors.lastname: Khaleel
authors.forename: A R N
authors.initials: AR
authors.lastname: Ibrahim
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism / Q000494:pharmacology*; D015415:Biomarkers / Q000097:blood; D005298:Fertility / Q000187:drug effects*; D005640:Follicle Stimulating Hormone / Q000097:blood; D005978:Glutathione / Q000097:blood; D005979:Glutathione Peroxidase / Q000097:blood; D006728:Hormones / Q000097:blood; D028322:Inhibin-beta Subunits / Q000097:blood; D015227:Lipid Peroxidation / Q000187:drug effects; D007986:Luteinizing Hormone / Q000097:blood; D029561:Lythraceae / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D000069463:Olive Oil; D018384:Oxidative Stress / Q000187:drug effects; D010938:Plant Oils / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D013076:Sperm Count; D013081:Sperm Motility / Q000187:drug effects*; D013094:Spermatozoa / Q000187:drug effects*; D013737:Testis / Q000187:drug effects; D013739:Testosterone / Q000097:blood; D029974:Carum
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D006728:Hormones; D000069463:Olive Oil; D010938:Plant Oils; D013739:Testosterone; D008315:Malondialdehyde; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone; D028322:Inhibin-beta Subunits; C104525:caraway oil; D005979:Glutathione Peroxidase; D005978:Glutathione
keywords: Antioxidant enzymes; FSH; Inhibin-B; LH; Lipid peroxidation; Nagilla sativa oil; Olive oil; Pomegranate extract; Sperm characteristics; Testosterone