title: An innovative quaternary ammonium methacrylate polymer can provide improved antimicrobial properties for a dental adhesive system.
abstract: A quaternary ammonium methacrylate polymer (QAMP) with antimicrobial potential was synthesized. The resulting product (QAMP) was characterized by FTIR spectroscopy, NMR spectroscopy, visible spectrophotometry, XRPD and TGA. The in vitro susceptibility tests against Streptococcus mutans of QAMP were investigated prior and after incorporation into a commercial adhesive system (Clearfil™ SE Bond). The release of quaternary ammonium compounds from the experimental adhesive system (Clearfil™ SE Bond + 5% QAMP) was performed during 1, 7, 14, 21 and 30 days. Spectroscopic data confirmed that QAMP was successfully obtained. Thermogravimetric analysis indicated that QAMP was heat stable. Prior incorporation into the adhesive system, QAMP revealed an inhibition halo of 18.33 ± 0.6 mm. By agar disk diffusion test, Clearfil™ SE Bond containing 5% QAMP presented an inhibition halo (16.67 ± 1.5 mm) similar to Clearfil™ Protect Bond (positive control, 17.00 ± 1.7, p = 0.815) and significantly higher than Clearfil™ SE Bond (negative control, 11.00 ± 1.0, p = 0.006). The minimum inhibitory/bactericidal concentrations for Clearfil™ SE Bond containing 5% QAMP were 20 μL mL(-1). The release of quaternary ammonium compounds from the experimental adhesive containing QAMP was very low (5.1%) when compared to Clearfil™ Protect Bond that released 47.2% of its quaternary ammonium monomer (MDPB) after 30 days. The QAMP can offer enhanced antimicrobial properties for self-etching adhesive systems.
authors.affiliation: Postgraduate Program in Dentistry, Department of Dentistry, State University of Ponta Grossa, 4748 Carlos Cavalcanti Ave, 84030-900 Ponta Grossa, Brazil.
authors.forename: Yasmine Mendes
authors.initials: YM
authors.lastname: Pupo
authors.forename: Paulo Vitor
authors.initials: PV
authors.lastname: Farago
authors.forename: Jessica Mendes
authors.initials: JM
authors.lastname: Nadal
authors.forename: Luis Antônio
authors.initials: LA
authors.lastname: Esmerino
authors.forename: Daniela Florencio
authors.initials: DF
authors.lastname: Maluf
authors.forename: Sônia Faria
authors.initials: SF
authors.lastname: Zawadzki
authors.forename: Milton Domingos
authors.initials: MD
authors.lastname: Michél
authors.forename: Fábio André
authors.initials: FA
authors.lastname: dos Santos
authors.forename: Osnara Maria Mongruel
authors.initials: OM
authors.lastname: Gomes
authors.forename: João Carlos
authors.initials: JC
authors.lastname: Gomes
mesh_terms: D000891:Anti-Infective Agents, Local / Q000737:chemistry* / Q000494:pharmacology*; D003738:Dental Cements / Q000737:chemistry* / Q000494:pharmacology; D008689:Methacrylates / Q000737:chemistry* / Q000494:pharmacology; D011108:Polymers; D000644:Quaternary Ammonium Compounds / Q000737:chemistry* / Q000494:pharmacology; D017550:Spectroscopy, Fourier Transform Infrared; D013291:Streptococcus / Q000187:drug effects / Q000378:metabolism
chemical_list: D000891:Anti-Infective Agents, Local; D003738:Dental Cements; D008689:Methacrylates; D011108:Polymers; D000644:Quaternary Ammonium Compounds