title: Effect of alpha lipoic acid on ifosfamide-induced central neurotoxicity in rats.
abstract: Ifosfamide (IFOS) which is a cytotoxic alkylating agent may cause central nervous system toxicity. Alpha-lipoic acid (ALA) has a strong antioxidant effects. We hypothesized that ALA could attenuate on ifosfamide-induced central neurotoxicity in rats. Rats were divided into Control, IFOS, ALA and IFOS+ALA groups. The toxic effects of IFOS were analyzed by oxidative parameters and caspase 3 immunohistochemical examinations of brain tissue. The catalase activity of IFOS group significantly reduced in comparison with control groups (p < 0.05). The malondialdehyde (MDA) level and protein carbonyl (PC) content in brain tissue were significantly higher in IFOS group than in the other groups (p < 0.05). ALA treatments significantly prevented the increase in MDA level (p < 0.001) and PC content (p < 0.05) in brain tissue. IFOS group showed profound activation of caspase 3. The control, ALA and IFOS+ALA groups did not show caspase 3 activation. It was concluded that ALA treatments may have beneficial effects protecting neurons from central neurotoxicity caused by IFOS.
authors.affiliation: 1Department of Clinical Biochemistry, Ankara Diskapi Yildirim Beyazit Education and Research Hospital , Ankara , Turkey.
authors.forename: Gulfer
authors.initials: G
authors.lastname: Ozturk
authors.forename: Zeynep
authors.initials: Z
authors.lastname: Ginis
authors.forename: Sefika Nur
authors.initials: SN
authors.lastname: Kurt
authors.forename: Aynur
authors.initials: A
authors.lastname: Albayrak
authors.forename: Sule
authors.initials: S
authors.lastname: Bilen
authors.forename: Ersin
authors.initials: E
authors.lastname: Fadillioglu
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology* / Q000627:therapeutic use; D001921:Brain / Q000187:drug effects* / Q000378:metabolism; D053148:Caspase 3 / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D007069:Ifosfamide / Q000037:antagonists & inhibitors / Q000633:toxicity*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism; D020258:Neurotoxicity Syndromes / Q000188:drug therapy*; D050050:Protein Carbonylation / Q000187:drug effects; D051381:Rats; D008063:Thioctic Acid / Q000494:pharmacology* / Q000627:therapeutic use
chemical_list: D000975:Antioxidants; D008315:Malondialdehyde; D008063:Thioctic Acid; D002374:Catalase; D053148:Caspase 3; D007069:Ifosfamide