title: Diagnostic implications of inconsistent results obtained with the Xpert MTB/Rif assay in detection of Mycobacterium tuberculosis isolates with an rpoB mutation associated with low-level rifampin resistance.
abstract: Xpert-MTB/Rif is one of the most frequently used molecular screening tests for multidrug-resistant tuberculosis worldwide. We report false-negative assay results in the presence of rpoB Leu533Pro, which is associated with low-level phenotypic rifampin resistance. Accurate and timely confirmation of rifampin susceptibility results obtained with Xpert-MTB/Rif is imperative.
authors.affiliation: Institut für Medizinische Mikrobiologie Universität Zürich, Zürich, Switzerland. asomoskoevi@imm.uzh.ch
authors.forename: Akos
authors.initials: A
authors.lastname: Somoskovi
authors.forename: Vanessa
authors.initials: V
authors.lastname: Deggim
authors.forename: Diana
authors.initials: D
authors.lastname: Ciardo
authors.forename: Guido V
authors.initials: GV
authors.lastname: Bloemberg
mesh_terms: D000368:Aged; D001426:Bacterial Proteins / Q000235:genetics*; D001431:Bacteriological Techniques / Q000379:methods; D012321:DNA-Directed RNA Polymerases; D024881:Drug Resistance, Bacterial; D005188:False Negative Reactions; D006801:Humans; D008297:Male; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D020125:Mutation, Missense; D009169:Mycobacterium tuberculosis / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification*; D018088:Tuberculosis, Multidrug-Resistant / Q000175:diagnosis* / Q000382:microbiology
chemical_list: D001426:Bacterial Proteins; C505805:rpoB protein, Mycobacterium tuberculosis; D012321:DNA-Directed RNA Polymerases