title: Development of a highly sensitive chemiluminescent assay for hydrogen peroxide under neutral conditions using acridinium ester and its application to an enzyme immunoassay.
abstract: We developed a highly sensitive chemiluminescent (CL) assay for hydrogen peroxide using 10-methyl-9-(phenoxycarbonyl) acridinium fluorosulfonate (PMAC) that produced chemiluminescence under neutral conditions and applied it to an enzyme immunoassay (EIA). One picomole of hydrogen peroxide could be detected using the optimized PMAC-CL method and 6.2 × 10(-20) mol β-D-galactosidase (β-gal) could be detected by combining an indoxyl derivative substrate and the proposed PMAC-CL method. This highly sensitive CL β-gal assay was applied to an EIA for thyroid-stimulating hormone (TSH) using β-gal as a label enzyme; 0.02-100.0 μU/mL TSH in human serum could be assayed directly and with high reproducibility.
authors.affiliation: School of Pharmacy, Showa University, Shinagawa-ku, Tokyo, 142-8555, Japan.
authors.forename: Hidetoshi
authors.initials: H
authors.lastname: Arakawa
authors.forename: Keiko
authors.initials: K
authors.lastname: Tsuruoka
authors.forename: Ken-ichi
authors.initials: K
authors.lastname: Ohno
authors.forename: Noriko
authors.initials: N
authors.lastname: Tajima
authors.forename: Hiromi
authors.initials: H
authors.lastname: Nagano
mesh_terms: D000166:Acridines / Q000032:analysis*; D006801:Humans; D006861:Hydrogen Peroxide / Q000032:analysis*; D007124:Immunoenzyme Techniques / Q000379:methods*; D008163:Luminescent Measurements / Q000379:methods*; D013388:Succinimides / Q000032:analysis*; D013972:Thyrotropin / Q000032:analysis; D001616:beta-Galactosidase / Q000737:chemistry
chemical_list: D000166:Acridines; D013388:Succinimides; C053333:4-(2-succinimidyloxycarbonylethyl)phenyl-10-methylacridinium-9-carboxylate; D013972:Thyrotropin; D006861:Hydrogen Peroxide; D001616:beta-Galactosidase
keywords: EIA; PMAC; TSH; chemiluminescence; hydrogen peroxide; β-D-galactosidase