title: Human carbonic anhydrase VII protects cells from oxidative damage.
abstract: Human carbonic anhydrase (hCA) VII is a cytosolic enzyme with high carbon dioxide hydration activity. Recently, S-glutathionylation of two cysteine residues from the enzyme was revealed, suggesting a new role as oxygen radical scavenger. We analyzed the effect of native and tetramutated hCA VII (all cysteines mutated into serines) in a eukaryotic system by stressing cells with an oxidant agent. Results clearly show that native hCA VII can protect cells from oxidative damage by preventing the apoptosis cascade and that cysteines play a leading role in this process. Our findings definitively confirm hCA VII protective role toward oxidative insult.
authors.forename: Rita
authors.initials: R
authors.lastname: Del Giudice
authors.forename: Daria M
authors.initials: DM
authors.lastname: Monti
authors.forename: Emanuela
authors.initials: E
authors.lastname: Truppo
authors.forename: Angela
authors.initials: A
authors.lastname: Arciello
authors.forename: Claudiu T
authors.initials: CT
authors.lastname: Supuran
authors.forename: Giuseppina
authors.initials: G
authors.lastname: De Simone
authors.forename: Simona M
authors.initials: SM
authors.lastname: Monti
mesh_terms: D015153:Blotting, Western; D002256:Carbonic Anhydrases / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D006367:HeLa Cells; D006801:Humans; D018384:Oxidative Stress / Q000502:physiology*
chemical_list: D002256:Carbonic Anhydrases; C477051:carbonic anhydrase VI