title: Modeling of the cell-electrode interface noise for microelectrode arrays.
abstract: Microelectrodes are widely used in the physiological recording of cell field potentials. As microelectrode signals are generally in the μV range, characteristics of the cell-electrode interface are important to the recording accuracy. Although the impedance of the microelectrode-solution interface has been well studied and modeled in the past, no effective model has been experimentally verified to estimate the noise of the cell-electrode interface. Also in existing interface models, spectral information is largely disregarded. In this work, we developed a model for estimating the noise of the cell-electrode interface from interface impedances. This model improves over existing noise models by including the cell membrane capacitor and frequency dependent impedances. With low-noise experiment setups, this model is verified by microelectrode array (MEA) experiments with mouse muscle myoblast cells. Experiments show that the noise estimated from this model has <;10% error, which is much less than estimations from existing models. With this model, noise of the cell-electrode interface can be estimated by simply measuring interface impedances. This model also provides insights for micro- electrode design to achieve good recording signal-to-noise ratio.
authors.affiliation: Department of Electronic and Computer Engineering, Hong Kong University of Science and Technology, Clearwater Bay, Kowloon, Hong Kong. guojing@ust.hk
authors.forename: Jing
authors.initials: J
authors.lastname: Guo
authors.forename: Jie
authors.initials: J
authors.lastname: Yuan
authors.forename: Mansun
authors.initials: M
authors.lastname: Chan
mesh_terms: D000818:Animals; D001698:Biomedical Engineering; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism*; D025802:Electric Capacitance; D017097:Electric Impedance; D055724:Electrophysiological Phenomena; D004867:Equipment Design; D051379:Mice; D008839:Microelectrodes; D008954:Models, Biological; D032446:Myoblasts / Q000378:metabolism