title: Increasing the density of markers around a major QTL controlling resistance to angular leaf spot in common bean.
abstract: Angular leaf spot (ALS) causes major yield losses in the common bean (Phaseolus vulgaris L.), an important protein source in the human diet. This study describes the saturation around a major quantitative trait locus (QTL) region, ALS10.1, controlling resistance to ALS located on linkage group Pv10 and explores the genomic context of this region using available data from the P. vulgaris genome sequence. DArT-derived markers (STS-DArT) selected by bulk segregant analysis and SCAR and SSR markers were used to increase the resolution of the QTL, reducing the confidence interval of ALS10.1 from 13.4 to 3.0 cM. The position of the SSR ATA220 coincided with the maximum LOD score of the QTL. Moreover, a new QTL (ALS10.2(UC)) was identified at the end of the same linkage group. Sequence analysis using the P. vulgaris genome located ten SSRs and seven STS-DArT on chromosome 10 (Pv10). Coincident linkage and genome positions of five markers enabled the definition of a core region for ALS10.1 spanning 5.3 Mb. These markers are linked to putative genes related to disease resistance such as glycosyl transferase, ankyrin repeat-containing, phospholipase, and squamosa-promoter binding protein. Synteny analysis between ALS10.1 markers and the genome of soybean suggested a dynamic evolution of this locus in the common bean. The present study resulted in the identification of new candidate genes and markers closely linked to a major ALS disease resistance QTL, which can be used in marker-assisted selection, fine mapping and positional QTL cloning.
authors.affiliation: Departamento de Genética e Evolução e Bioagentes, Instituto de Biologia, Universidade Estadual de Campinas (UNICAMP), Campinas, São Paulo, 13083-970, Brazil, parobl@gmail.com.
authors.forename: Paula Rodrigues
authors.initials: PR
authors.lastname: Oblessuc
authors.forename: Juliana Morini Kupper
authors.initials: JM
authors.lastname: Cardoso Perseguini
authors.forename: Renata Moro
authors.initials: RM
authors.lastname: Baroni
authors.forename: Alisson Fernando
authors.initials: AF
authors.lastname: Chiorato
authors.forename: Sérgio Augusto Morais
authors.initials: SA
authors.lastname: Carbonell
authors.forename: Jorge Mauricio Costa
authors.initials: JM
authors.lastname: Mondego
authors.forename: Ramon Oliveira
authors.initials: RO
authors.lastname: Vidal
authors.forename: Luis Eduardo Aranha
authors.initials: LE
authors.lastname: Camargo
authors.forename: Luciana Lasry
authors.initials: LL
authors.lastname: Benchimol-Reis
mesh_terms: D032461:Chromosomes, Plant / Q000235:genetics; D060467:Disease Resistance / Q000235:genetics*; D020440:Gene Duplication / Q000235:genetics; D017343:Genes, Plant / Q000235:genetics; D008040:Genetic Linkage; D005819:Genetic Markers; D005838:Genotype; D006801:Humans; D018895:Microsatellite Repeats / Q000235:genetics; D027805:Phaseolus / Q000235:genetics* / Q000276:immunology / Q000382:microbiology*; D010935:Plant Diseases / Q000235:genetics* / Q000276:immunology / Q000382:microbiology; D018515:Plant Leaves / Q000235:genetics* / Q000276:immunology / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D040641:Quantitative Trait Loci / Q000235:genetics*; D013025:Glycine max / Q000235:genetics; D026801:Synteny / Q000235:genetics
chemical_list: D005819:Genetic Markers