title: Decontamination of Escherichia coli O157:H7 on green onions using pulsed light (PL) and PL-surfactant-sanitizer combinations.
abstract: Imported green onion has been associated with three large outbreaks in the USA. Contamination has been found on both domestic and imported green onions. The objective of our study was to investigate Escherichia coli O157:H7 inactivation efficacy of pulsed light (PL) as well as its combination with surfactant and/or sanitizers on green onions. Green onions were cut into two segments, stems and leaves, to represent two different matrixes. Stems were more difficult to be decontaminated. Spot and dip inoculation methods were compared and dipped inoculated green onions were found to be more difficult to be decontaminated. Results showed that 5s dry PL (samples were not immersed in water during PL treatment) and 60s wet PL (samples were immersed in water and stirred during PL treatment) treatments provided promising inactivation efficacy (>4log10CFU/g) for spot inoculated stems and leaves. For dip inoculated green onions, 60s wet PL treatment was comparable with 100ppm chlorine washing, demonstrating that PL could be used as an alternative to chlorine. To further increase the degree of microbial inactivation, combined treatments were applied. PL combined with surfactant (SDS) was found to be more effective than single treatments of PL, SDS, chlorine, citric acid, thymol, and hydrogen peroxide, and binary combined treatments of PL with one of those chemicals. Addition of chlorine or hydrogen peroxide to the PL-SDS combination did not further enhanced its microbial inactivation efficacy. The combination of PL and 1000ppm of SDS reduced the E. coli O157:H7 populations dip inoculated on the stems and leaves of green onions by 1.4 and 3.1log10CFU/g, respectively. Our findings suggest that PL could potentially be used for decontamination of E. coli O157:H7 on green onions, with wet PL added with SDS being the most effective PL treatment.
authors.affiliation: Department of Animal and Food Sciences, University of Delaware, Newark, DE 19716, USA.
authors.forename: Wenqing
authors.initials: W
authors.lastname: Xu
authors.forename: Haiqiang
authors.initials: H
authors.lastname: Chen
authors.forename: Yaoxin
authors.initials: Y
authors.lastname: Huang
authors.forename: Changqing
authors.initials: C
authors.lastname: Wu
mesh_terms: D002713:Chlorine / Q000494:pharmacology*; D015169:Colony Count, Microbial; D003666:Decontamination / Q000379:methods*; D004202:Disinfectants / Q000494:pharmacology; D019453:Escherichia coli O157 / Q000187:drug effects / Q000528:radiation effects; D005511:Food Handling / Q000379:methods; D005516:Food Microbiology / Q000379:methods*; D008027:Light; D050296:Microbial Viability; D019697:Onions / Q000382:microbiology*; D013501:Surface-Active Agents / Q000494:pharmacology*
chemical_list: D004202:Disinfectants; D013501:Surface-Active Agents; D002713:Chlorine
keywords: E. coli O157:H7; Green onions; Pulsed light (PL); SDS