title: [Analysis the viral etiology of fever and respiratory tract infection syndrome in Shaanxi province].
abstract: OBJECTIVE
  Analysis the viral pathogenic spectrum for patients with fever and respiratory tract infection syndrome in Shaanxi province during 2010 and investigate the molecular epidemiology characteristics of respiratory syncytial virus.
  
  
  METHODS
  A total of 208 patients' pharyngeal swabs were collected based on surveillance definition from January 2010 to January 2011 and screened for sixteen human respiratory virus types/subtypes by Qiaxcel-based multiplex reverse transcription-PCR assay, including HRV,HCoV, Flu, HPIV, ADV, HRSV, HMPV and HBoV and investigate molecular epidemiology of HRSV by sequencing and phylogenetic analysis of the C-terminal second hypervariable region of the G gene.
  
  
  RESULTS
  109 out of 208 specimens (53%) were positive for one or more viruses. HRSV(42. 2%) was the dominant pathogen detected, followed by Flu(24. 5%), PIV(20%), HRV(13.6%) and ADV( 10.9%),there were also 8 strains of HCoV, 5 strains of HMPV and 3 strains of HBoV detected. The results showed that 22 specimens were positive for two or more viruses, PIV (14/22) was the most frequently detected viral agent among co-infection specimens, and the highest incidence of mixed infection is aged 15-39 years group (P < 0.05). The overall viral detection rate was no related to age. In addition to Flu, HMPV and PIV, other viruses (HRV, HBoV, HCoV, ADV, RSV) mainly infected 0 to 4 years old children. Among 46 HRSV positive specimens, 42 HRSV-A strains clustered into NA1 genotype and two HRSV-B strains clustered into two genotypes, BA9 and GB2.
  
  
  CONCLUSIONS
  HRSV is the dominate pathogen collected from patients with fever and respiratory tract infection syndrome in Shaanxi and HRSV A is the predominant subtype. For most viruses, infection was most prevalent among children aged <4 years. PIV was the most common pathogen in co-infection.
authors.affiliation: Department of Immunology Laboratory in Pediatric Research Institute, Beijing Children's Hospital affiliated to Capital Medical University, Beijing, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Shi
authors.forename: Peng-bo
authors.initials: PB
authors.lastname: Yu
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.forename: Ai-li
authors.initials: AL
authors.lastname: Cui
authors.forename: Jing
authors.initials: J
authors.lastname: Xu
authors.forename: Guo-hong
authors.initials: GH
authors.lastname: Huang
authors.forename: Wei
authors.initials: W
authors.lastname: Shi
authors.forename: Nai-ying
authors.initials: NY
authors.lastname: Mao
authors.forename: Wen-bo
authors.initials: WB
authors.lastname: Xu
authors.forename: Jing-jun
authors.initials: JJ
authors.lastname: Wang
authors.forename: Xiao-hui
authors.initials: XH
authors.lastname: Kong
mesh_terms: D000293:Adolescent; D000328:Adult; D002681:China / Q000453:epidemiology; D060085:Coinfection / Q000821:virology; D005260:Female; D005334:Fever / Q000453:epidemiology* / Q000821:virology*; D005838:Genotype; D006801:Humans; D008297:Male; D010802:Phylogeny; D018357:Respiratory Syncytial Virus Infections / Q000453:epidemiology* / Q000821:virology*; D018113:Respiratory Syncytial Virus, Human / Q000302:isolation & purification*; D012141:Respiratory Tract Infections / Q000453:epidemiology* / Q000821:virology*; D055815:Young Adult