title: CEACAM6 induces epithelial-mesenchymal transition and mediates invasion and metastasis in pancreatic cancer.
abstract: Pancreatic cancer is a disease with an extremely poor prognosis. The acquisition of invasion properties in pancreatic cancer is accompanied by the process of epithelial-mesenchymal transition (EMT). Carcinoembryonic antigen-related cell adhesion molecule 6 (CEACAM6) is emerging as an important determinant of the malignant phenotype in a range of cancers, including pancreatic cancer. Therefore, the aim of this study was to evaluate the potential involvement of CEACAM6 in the invasion and metastasis of pancreatic cancer cells via EMT regulation. The results of our study showed a positive association between CEACAM6 expression and poor prognosis of pancreatic cancer, differentiation and lymph node metastasis. Elevated levels of CEACAM6 in pancreatic cancer cells promoted EMT, migration and invasion in vitro and metastasis in animal models, whereas shRNA-mediated CEACAM6 knockdown had the opposite effect. Furthermore, we demonstrated that miR-29a/b/c specific for CEACAM6 could regulate its expression at the post-transcriptional level. Collectively, our findings identified CEACAM6, which is regulated by miR-29a/b/c, as an important positive regulator of EMT in pancreatic cancer offering an explanation for how elevated levels of CEACAM6 are likely to contribute to the highly metastatic phenotype of pancreatic cancer.
authors.affiliation: Department of General Surgery, The First Affiliated Hospital of Nanjing Medical University, Nanjing, P.R. China.
authors.forename: Jianmin
authors.initials: J
authors.lastname: Chen
authors.forename: Qiang
authors.initials: Q
authors.lastname: Li
authors.forename: Yong
authors.initials: Y
authors.lastname: An
authors.forename: Nan
authors.initials: N
authors.lastname: Lv
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Xue
authors.forename: Jishu
authors.initials: J
authors.lastname: Wei
authors.forename: Kuirong
authors.initials: K
authors.lastname: Jiang
authors.forename: Junli
authors.initials: J
authors.lastname: Wu
authors.forename: Wentao
authors.initials: W
authors.lastname: Gao
authors.forename: Zhuyin
authors.initials: Z
authors.lastname: Qian
authors.forename: Cuncai
authors.initials: C
authors.lastname: Dai
authors.forename: Zekuan
authors.initials: Z
authors.lastname: Xu
authors.forename: Yi
authors.initials: Y
authors.lastname: Miao
mesh_terms: D015703:Antigens, CD / Q000096:biosynthesis / Q000235:genetics*; D015815:Cell Adhesion Molecules / Q000096:biosynthesis / Q000235:genetics*; D045744:Cell Line, Tumor; D002465:Cell Movement; D058750:Epithelial-Mesenchymal Transition / Q000235:genetics*; D058851:GPI-Linked Proteins / Q000096:biosynthesis / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D055785:Gene Knockdown Techniques; D006801:Humans; D035683:MicroRNAs / Q000235:genetics; D009361:Neoplasm Invasiveness / Q000235:genetics / Q000473:pathology; D009362:Neoplasm Metastasis / Q000235:genetics* / Q000473:pathology; D010190:Pancreatic Neoplasms / Q000235:genetics* / Q000473:pathology; D034741:RNA, Small Interfering / Q000235:genetics
chemical_list: D015703:Antigens, CD; C504117:CEACAM6 protein, human; D015815:Cell Adhesion Molecules; D058851:GPI-Linked Proteins; C532045:MIRN29a microRNA, human; D035683:MicroRNAs; D034741:RNA, Small Interfering