title: Ameliorative effects of L-carnitine and vitamin E (α-tocopherol) on haematological and serum biochemical parameters in White Leghorn cockerels given ochratoxin A contaminated feed.
abstract: 1. L-carnitine is a quaternary ammonium compound biologically synthesised from the amino acids methionine and lysine while vitamin E (α-tocopherol) is an important antioxidant. The objective of the present study was to evaluate the ameliorative effects of L-carnitine and vitamin E upon haematological and serum biochemical parameters in ochratoxin A intoxicated birds. 2. Day-old White Leghorn cockerels were acclimatised for 2 d, divided in 12 groups with 20 birds in each group. From d 3 of age, they were given different combinations of ochratoxin A (1.0 and 2.0 mg/kg), L-carnitine (1 g/kg) and vitamin E (200 mg/kg) in feed. Haematological (erythrocyte count, leucocyte count, haemoglobin concentration and haematocrit percentage) and serum biochemical parameters (serum urea, creatinine, albumin, total proteins and alanine aminotransferase) were evaluated. 3. Results confirmed that L-carnitine and vitamin E given alone or combined with 1.0 mg/kg ochratoxin A ameliorated toxin induced alterations in haematological and serum biochemical parameters. This amelioration, however, did not occur when ochratoxin of 2.0 mg/kg was given. 4. L-carnitine and vitamin E in combination have the ability to ameliorate ochratoxin altered haematological and serum biochemical parameters. However, the optimum ratio of L-carnitine + vitamin E, to be used to assure such mitigation of ochratoxin A altered changes in haematological and serum biochemical parameters in cockerels, has yet to be determined. The combination used in this study was indeed sufficient to ameliorate the alterations induced by ochratoxin A up to 1.0 mg/kg feed.
authors.affiliation: Veterinary Research Institute, Zarrar Shaheed Road, Lahore Cantt-13, Pakistan. ranazain848@yahoo.com
authors.forename: Z
authors.initials: Z
authors.lastname: Abidin
authors.forename: M Z
authors.initials: MZ
authors.lastname: Khan
authors.forename: A
authors.initials: A
authors.lastname: Khatoon
authors.forename: M K
authors.initials: MK
authors.lastname: Saleemi
authors.forename: A
authors.initials: A
authors.lastname: Khan
authors.forename: I
authors.initials: I
authors.lastname: Javed
mesh_terms: D000821:Animal Feed / Q000032:analysis; D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage / Q000494:pharmacology*; D001774:Blood Chemical Analysis / Q000662:veterinary; D002331:Carnitine / Q000008:administration & dosage / Q000494:pharmacology*; D002645:Chickens / Q000502:physiology*; D004032:Diet / Q000662:veterinary; D006403:Hematologic Tests / Q000662:veterinary; D008297:Male; D009793:Ochratoxins / Q000008:administration & dosage / Q000633:toxicity*; D024502:alpha-Tocopherol / Q000008:administration & dosage / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D009793:Ochratoxins; C025589:ochratoxin A; D024502:alpha-Tocopherol; D002331:Carnitine