title: MC3T3-E1 and RAW264.7 cell response to hydroxyapatite and alpha-type alumina adsorbed with bovine serum albumin.
abstract: Initial cell responses following implantation are important for inducing osteoconductivity. We investigated cell adhesion, spreading, and proliferation in response to native and bovine serum albumin (BSA)-adsorbed disc of hydroxyapatite (HA) or alpha-type alumina (α-Al2O3) using mouse MC3T3-E1 osteoblastic cells and mouse RAW264.7 macrophages. The adsorbed BSA inhibited adhesion and spreading of MC3T3-E1 cells, but did not affect MC3T3-E1 cell proliferation on HA and α-Al2O3 substrates. Thus, MC3T3-E1 cells quickly adhere to original HA before cell binding is impeded by adsorption of BSA in quantities sufficient to inhibit the adhesion of MC3T3-E1 cells. The adsorbed BSA inhibits adhesion of RAW264.7 cells to α-Al2O3, but not to HA. BSA adsorption does not affect RAW264.7 cell spreading and proliferation on both HA and α-Al2O3 substrates. Thus, BSA adsorbed on HA stimulates a different cell response than α-Al2O3. Moreover, quick adherence of osteoblast cells and monocyte-macrophage lineage cells plays a role in HA osteoconductivity.
authors.affiliation: Graduate School of Biomedical Engineering, Tohoku University, 6-6-11-901-4 Aramaki-Aoba, Aoba-ku, Sendai, 980-8579, Japan.
authors.forename: Masakazu
authors.initials: M
authors.lastname: Kawashita
authors.forename: Jumpei
authors.initials: J
authors.lastname: Hayashi
authors.forename: Tada-aki
authors.initials: TA
authors.lastname: Kudo
authors.forename: Hiroyasu
authors.initials: H
authors.lastname: Kanetaka
authors.forename: Zhixia
authors.initials: Z
authors.lastname: Li
authors.forename: Toshiki
authors.initials: T
authors.lastname: Miyazaki
authors.forename: Masami
authors.initials: M
authors.lastname: Hashimoto
mesh_terms: D000327:Adsorption; D000537:Aluminum Oxide / Q000737:chemistry*; D000818:Animals; D002417:Cattle; D002448:Cell Adhesion; D002454:Cell Differentiation; D002460:Cell Line; D049109:Cell Proliferation; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D017886:Durapatite / Q000737:chemistry*; D008264:Macrophages / Q000166:cytology*; D051379:Mice; D010006:Osteoblasts / Q000166:cytology*; D012710:Serum Albumin, Bovine / Q000737:chemistry*
chemical_list: D020099:Coated Materials, Biocompatible; D012710:Serum Albumin, Bovine; D017886:Durapatite; D000537:Aluminum Oxide
keywords: MC3T3-E1; RAW264.7; alumina; bovine serum albumin; hydroxyapatite