title: Neuroprotective effect of tea polyphenols on oxyhemoglobin induced subarachnoid hemorrhage in mice.
abstract: Tea polyphenols are of great benefit to the treatment of several neurodegenerative diseases. In order to explore the neuroprotective effects of tea polyphenols and their potential mechanisms, an established in vivo subarachnoid hemorrhage (SAH) model was used and alterations of mitochondrial function, ATP content, and cytochrome c (cyt c) in cerebral cortex were detected. This study showed that the alteration of mitochondrial membrane potential was an early event in SAH progression. The trend of ATP production was similar to that of mitochondrial membrane potential, indicating that the lower the mitochondrial membrane potential, lesser the ATP produced. Due to mitochondrial dysfunction, more cyt c was released in the SAH group. Interestingly, the preadministration of tea polyphenols significantly rescued the mitochondrial membrane potential to basal level, as well as the ATP content and the cyt c level in the brain cortex 12 h after SAH. After pretreatment with tea polyphenols, the neurological outcome was also improved. The results provide strong evidence that tea polyphenols enhance neuroprotective effects by inhibiting polarization of mitochondrial membrane potential, increasing ATP content, and blocking cyt c release.
authors.affiliation: Department of Food Science, Henan Institute of Science and Technology, Xinxiang, Henan 453003, China.
authors.forename: Haizhen
authors.initials: H
authors.lastname: Mo
authors.forename: Ying
authors.initials: Y
authors.lastname: Chen
authors.forename: Liyong
authors.initials: L
authors.lastname: Huang
authors.forename: Hao
authors.initials: H
authors.lastname: Zhang
authors.forename: Juxiang
authors.initials: J
authors.lastname: Li
authors.forename: Wenke
authors.initials: W
authors.lastname: Zhou
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D001930:Brain Injuries / Q000188:drug therapy / Q000209:etiology; D002540:Cerebral Cortex / Q000187:drug effects / Q000473:pathology; D045304:Cytochromes c / Q000378:metabolism; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D051379:Mice; D018696:Neuroprotective Agents / Q000494:pharmacology / Q000627:therapeutic use*; D010108:Oxyhemoglobins / Q000009:adverse effects*; D059808:Polyphenols / Q000494:pharmacology / Q000627:therapeutic use*; D013345:Subarachnoid Hemorrhage / Q000139:chemically induced* / Q000150:complications / Q000188:drug therapy*; D013662:Tea / Q000737:chemistry*
chemical_list: D018696:Neuroprotective Agents; D010108:Oxyhemoglobins; D059808:Polyphenols; D013662:Tea; D000255:Adenosine Triphosphate; D045304:Cytochromes c