title: Transcriptome profiling of radish (Raphanus sativus L.) root and identification of genes involved in response to Lead (Pb) stress with next generation sequencing.
abstract: Lead (Pb), one of the most toxic heavy metals, can be absorbed and accumulated by plant roots and then enter the food chain resulting in potential health risks for human beings. The radish (Raphanus sativus L.) is an important root vegetable crop with fleshy taproots as the edible parts. Little is known about the mechanism by which radishes respond to Pb stress at the molecular level. In this study, Next Generation Sequencing (NGS)-based RNA-seq technology was employed to characterize the de novo transcriptome of radish roots and identify differentially expressed genes (DEGs) during Pb stress. A total of 68,940 assembled unique transcripts including 33,337 unigenes were obtained from radish root cDNA samples. Based on the assembled de novo transcriptome, 4,614 DEGs were detected between the two libraries of untreated (CK) and Pb-treated (Pb1000) roots. Gene Ontology (GO) and pathway enrichment analysis revealed that upregulated DEGs under Pb stress are predominately involved in defense responses in cell walls and glutathione metabolism-related processes, while downregulated DEGs were mainly involved in carbohydrate metabolism-related pathways. The expression patterns of 22 selected genes were validated by quantitative real-time PCR, and the results were highly accordant with the Solexa analysis. Furthermore, many candidate genes, which were involved in defense and detoxification mechanisms including signaling protein kinases, transcription factors, metal transporters and chelate compound biosynthesis related enzymes, were successfully identified in response to heavy metal Pb. Identification of potential DEGs involved in responses to Pb stress significantly reflected alterations in major biological processes and metabolic pathways. The molecular basis of the response to Pb stress in radishes was comprehensively characterized. Useful information and new insights were provided for investigating the molecular regulation mechanism of heavy metal Pb accumulation and tolerance in root vegetable crops.
authors.affiliation: National Key Laboratory of Crop Genetics and Germplasm Enhancement, Engineering Research Center of Horticultural Crop Germplasm Enhancement and Utilization, Ministry of Education of PR China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.forename: Liang
authors.initials: L
authors.lastname: Xu
authors.forename: Yinglong
authors.initials: Y
authors.lastname: Chen
authors.forename: Hong
authors.initials: H
authors.lastname: Shen
authors.forename: Yiqin
authors.initials: Y
authors.lastname: Gong
authors.forename: Cecilia
authors.initials: C
authors.lastname: Limera
authors.forename: Liwang
authors.initials: L
authors.lastname: Liu
mesh_terms: D018556:Crops, Agricultural / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D018744:DNA, Plant / Q000235:genetics; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D063990:Gene Ontology; D017343:Genes, Plant; D005978:Glutathione / Q000378:metabolism; D059014:High-Throughput Nucleotide Sequencing; D007854:Lead / Q000494:pharmacology*; D053858:Metabolic Networks and Pathways; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D018517:Plant Roots / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D031224:Raphanus / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D017422:Sequence Analysis, DNA; D012989:Soil Pollutants / Q000494:pharmacology*; D013312:Stress, Physiological; D059467:Transcriptome / Q000187:drug effects*
chemical_list: D018744:DNA, Plant; D010940:Plant Proteins; D012989:Soil Pollutants; D007854:Lead; D005978:Glutathione