title: The miRNA and mRNA Signatures of Peripheral Blood Cells in Humans Infected with Trypanosoma brucei gambiense.
abstract: Simple, reliable tools for diagnosis of human African Trypanosomiases could ease field surveillance and enhance patient care. In particular, current methods to distinguish patients with (stage II) and without (stage I) brain involvement require samples of cerebrospinal fluid. We describe here an exploratory study to find out whether miRNAs from peripheral blood leukocytes might be useful in diagnosis of human trypanosomiasis, or for determining the stage of the disease. Using microarrays, we measured miRNAs in samples from Trypanosoma brucei gambiense-infected patients (9 stage I, 10 stage II), 8 seronegative parasite-negative controls and 12 seropositive, but parasite-negative subjects. 8 miRNAs (out of 1205 tested) showed significantly lower expression in patients than in seronegative, parasite-negative controls, and 1 showed increased expression. There were no clear differences in miRNAs between patients in different disease stages. The miRNA profiles could not distinguish seropositive, but parasitologically negative samples from controls and results within this group did not correlate with those from the trypanolysis test. Some of the regulated miRNAs, or their predicted mRNA targets, were previously reported changed during other infectious diseases or cancer. We conclude that the changes in miRNA profiles of peripheral blood lymphocytes in human African trypanosomiasis are related to immune activation or inflammation, are probably disease-non-specific, and cannot be used to determine the disease stage. The approach has little promise for diagnostics but might yield information about disease pathology.
authors.affiliation: Division of Functional Genome Analysis, Deutsche Krebsforschungszentrum (DKFZ), Heidelberg, Germany.
authors.forename: Smiths
authors.initials: S
authors.lastname: Lueong
authors.forename: Smiths
authors.initials: S
authors.lastname: Leong
authors.forename: Gustave
authors.initials: G
authors.lastname: Simo
authors.forename: Mamadou
authors.initials: M
authors.lastname: Camara
authors.forename: Vincent
authors.initials: V
authors.lastname: Jamonneau
authors.forename: Jacques
authors.initials: J
authors.lastname: Kabore
authors.forename: Hamidou
authors.initials: H
authors.lastname: Ilboudo
authors.forename: Bruno
authors.initials: B
authors.lastname: Bucheton
authors.forename: Jörg D
authors.initials: JD
authors.lastname: Hoheisel
authors.forename: Christine
authors.initials: C
authors.lastname: Clayton
mesh_terms: D015415:Biomarkers / Q000097:blood; D020869:Gene Expression Profiling; D006801:Humans; D007962:Leukocytes / Q000378:metabolism; D035683:MicroRNAs / Q000097:blood*; D046228:Microarray Analysis; D008958:Models, Molecular; D012333:RNA, Messenger / Q000097:blood*; D060888:Real-Time Polymerase Chain Reaction; D059467:Transcriptome; D014347:Trypanosoma brucei gambiense; D014353:Trypanosomiasis, African / Q000097:blood*
chemical_list: D015415:Biomarkers; D035683:MicroRNAs; D012333:RNA, Messenger