title: Expression of Das-1, Ki67 and sulfuric proteins in gastric cardia adenocarcinoma and intestinal metaplasia lesions.
abstract: The aim of this study was to characterize histo-chemical patterns and Das-1 and Ki67 protein expression in gastric cardia adenocarcinoma (GCA) and intestinal metaplasia (IM) lesions adjacent to GCA. Histochemical techniques, including Alcian blue/periodic acid-Schiff (AB/PAS), high iron diamine/Alcian blue (HID/AB) and avidin-biotin-peroxidase complex (ABC) immunohistochemistry were applied to GCA and IM samples from patients (n=200) in Linzhou, Henan, China, a high incidence area for GCA and esophageal squamous cell carcinoma (SCC). The detection rate of IM lesions in resected tissues adjacent to GCA was 32.5% (65/200). GCA and IM lesions presented a high frequency of Das-1 and Ki67-positive staining with statistical significance (P<0.01). The expression of sulfuric proteins did not show co-expression with Das-1 and Ki67 in GCA and surrounding IM lesions (P>0.05) from the same GCA patient. The high frequency of co-expression of Das-1 and Ki67 in GCA and adjacent IM lesions indicates that IM adjacent to GCA may undergo similar molecular changes to GCA, which may be one of the mechanisms for malignant transformation of IM in the population studied.
authors.affiliation: Department of Oncology, Cancer Institute, The First Affiliated Hospital of Henan University of Science and Technology, Luoyang, Henan 471003;
authors.forename: Xiao-Shan
authors.initials: XS
authors.lastname: Feng
authors.forename: Yu-Feng
authors.initials: YF
authors.lastname: Wang
authors.forename: Shu-Guang
authors.initials: SG
authors.lastname: Hao
authors.forename: Yi
authors.initials: Y
authors.lastname: Ru
authors.forename: She-Gan
authors.initials: SG
authors.lastname: Gao
authors.forename: Li-Dong
authors.initials: LD
authors.lastname: Wang
keywords: Das-1; Ki67; gastric cardia adenocarcinoma; histochemistry; immunohistochemistry; intestinal metaplasia