title: Humibacter antri sp. nov., an actinobacterium isolated from a natural cave, and emended description of the genus Humibacter.
abstract: A novel high DNA G+C content bacterium, designated strain D7-27(T), was isolated from clay soils collected inside a natural cave in Jeju, Republic of Korea. The cells of the organism were aerobic, Gram-stain-positive, non-motile rods; its colonies were white, circular and entire in margin. Phylogenetic analyses based on 16S rRNA gene sequence comparison showed that strain D7-27(T) formed a coherent cluster with Humibacter albus of the family Microbacteriaceae (97.6 % sequence similarity). The cell-wall peptidoglycan contained ornithine and 2,4-diaminobutyric acid as the diagnostic diamino acids. The major menaquinones were MK-12 and MK-11, with MK-10 as a minor component. The polar lipids consisted mainly of phosphatidylglycerol and an unknown glycolipid. Mycolic acids were not present. The predominant fatty acids were anteiso-C17 : 0 and cyclohexyl-C17 : 0. The DNA G+C content was 66.3 mol%. DNA-DNA relatedness between strain D7-27(T) and H. albus DSM 18994(T) was 28.6 % (17.6 % in a reciprocal test). On the basis of the phenotypic, chemotaxonomic and DNA-DNA hybridization data, strain D7-27(T) ( = KCTC 33009(T) = DSM 25738(T)) is considered as the type strain of a novel species of the genus Humibacter, for which the name Humibacter antri sp. nov. is proposed. An emended description of the genus Humibacter is also provided.
authors.affiliation: Department of Science Education, Jeju National University, Jeju 690-756, Republic of Korea.
authors.forename: Soon Dong
authors.initials: SD
authors.lastname: Lee
mesh_terms: D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D000971:Antineoplastic Combined Chemotherapy Protocols; D001482:Base Composition; D059552:Caves / Q000382:microbiology*; D002473:Cell Wall / Q000737:chemistry; D003520:Cyclophosphamide; D004269:DNA, Bacterial / Q000235:genetics; D004317:Doxorubicin; D005227:Fatty Acids / Q000737:chemistry; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010457:Peptidoglycan / Q000737:chemistry; D010715:Phosphatidylglycerols / Q000737:chemistry; D010802:Phylogeny; D011034:Podophyllotoxin; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D012988:Soil Microbiology; D014750:Vincristine; D024482:Vitamin K 2 / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010457:Peptidoglycan; D010715:Phosphatidylglycerols; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; D014750:Vincristine; D004317:Doxorubicin; D003520:Cyclophosphamide; D011034:Podophyllotoxin