title: Immunopotentiation of outer membrane protein through anti-idiotype Pasteurella multocida vaccine in rabbits.
abstract: Pasteurella multocida was isolated from cattle affected with haemorrhagic septicaemia and characterized on the basis of morphological, cultural and biochemical tests. Bacterial outer membrane proteins (OMPs) were extracted with 1% Sarkosyl method. P. multocida anti-idiotype vaccine prepared from OMPs (21.3 mg per 100 ml), was evaluated and compared with bacterin supplemented with 10% OMPs and plain alum-adsorbed bacterin in rabbit models. It was observed that OMPs-anti-idiotype vaccine induced high levels of antibody titres (geomean titres -GMT) detected using indirect haemagglutination (IHA) test. The OMPs anti-idiotype antibody titres of 168.9 GMT were obtained to 42.2 GMT in OMPs supplemented bacterin on 21 days post vaccination, while the plain bacterin had the least titre of 27.9 GMT. The OMPs-anti-idiotype vaccine provoked better immunogenic response in terms of highest GMT titres and long lasting effect in rabbits and 100% protection against the challenge with homologous strain of P. multocida,while 88% protection was obtained in rabbits, given OMPs supplemented bacterin.
authors.affiliation: Institute of Microbiology, University of Agriculture, Faisalabad 38000 Pakistan. Electronic address: javidarif@yahoo.com.
authors.forename: Javid
authors.initials: J
authors.lastname: Arif
authors.forename: Sajjad-Ur
authors.initials: SU
authors.lastname: Rahman
authors.forename: Muhammad
authors.initials: M
authors.lastname: Arshad
authors.forename: Pervez
authors.initials: P
authors.lastname: Akhtar
mesh_terms: D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000097:blood / Q000276:immunology*; D000907:Antibodies, Bacterial / Q000097:blood / Q000276:immunology*; D000942:Antigens, Bacterial / Q000008:administration & dosage / Q000276:immunology; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology*; D001428:Bacterial Vaccines / Q000008:administration & dosage / Q000276:immunology; D002417:Cattle; D006386:Hemagglutination Tests; D006483:Hemorrhagic Septicemia / Q000276:immunology / Q000382:microbiology; D008297:Male; D016979:Pasteurella multocida / Q000145:classification / Q000276:immunology*; D011817:Rabbits; D012703:Serotyping; D013997:Time Factors; D014611:Vaccination
chemical_list: D000888:Antibodies, Anti-Idiotypic; D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001428:Bacterial Vaccines
keywords: Anti-idiotype vaccine; Indirect haemagglutination test; Outer membrane proteins; Pasteurella multocida