title: Molecular characterization of bacterial and archaeal communities in a full-scale anaerobic reactor treating corn straw.
abstract: A 16S rRNA gene-based method was used to characterize the structure of bacterial and archaeal communities in a full-scale, anaerobic reactor treating corn straw. Degradability experiment indicated biogas slurry had high microbial activity, the TS removal rate was 53% and the specific methanogenic activity was 86 mL CH4 g VSS(-1) d(-1). During anaerobic degradation of corn straw, volatile acids and aromatic compounds (p-cresol, phenylpropionate, phenol and benzoate) were detected as transient intermediates. Phylogenetic analysis revealed bacterial community exhibited high diversity, 69 bacterial phylotypes in 13 phyla were identified. Firmicutes (48.3%), Chloroflexi (20.1%), Actinobacteria (9.1%), Bacteroidetes (7.7%), and Proteobacteria (7.2%) represented the most abundant bacterial phyla. Hydrolytic and fermentative bacteria were major bacterial populations. Moreover, a relatively high proportion of syntrophic propionate and aromatic compounds degrading bacteria were detected. In the archaeal clone library, 11 archaeal phylotypes affiliated with two phyla of Crenarchaeota (10%) and Euryarchaeota (90%) were identified.
authors.affiliation: Key Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, Shandong Province 266101, PR China.
authors.forename: Jiang-Tao
authors.initials: JT
authors.lastname: Qiao
authors.forename: Yan-Ling
authors.initials: YL
authors.lastname: Qiu
authors.forename: Xian-Zheng
authors.initials: XZ
authors.lastname: Yuan
authors.forename: Xiao-Shuang
authors.initials: XS
authors.lastname: Shi
authors.forename: Xiao-Hui
authors.initials: XH
authors.lastname: Xu
authors.forename: Rong-Bo
authors.initials: RB
authors.lastname: Guo
mesh_terms: D000693:Anaerobiosis; D001105:Archaea / Q000235:genetics / Q000378:metabolism*; D001419:Bacteria / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D019149:Bioreactors; D017931:DNA Primers; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D003313:Zea mays
chemical_list: D017931:DNA Primers; D012336:RNA, Ribosomal, 16S
keywords: 16S rRNA gene clone library; Anaerobic degradation; Corn straw; Microbial community