title: Metabolic glycoengineering of Staphylococcus aureus reduces its adherence to human T24 bladder carcinoma cells.
abstract: The Gram-positive bacterium Staphylococcus aureus is a human pathogen increasingly causing severe infections, especially in hospital environments. Moreover, strains which are resistant against various types of antibiotics are developing and spreading widely as in the case of the community-acquired MRSA (methicillin resistant S. aureus). In this study metabolic glycoengineering with N-azidoacetyl-glucosamine (GlcNAz) has been successfully applied to S. aureus for the first time. The following bioorthogonal Mendal-Sharpless-Huisgen click reaction between the azido-functionalized S. aureus cells and alkyne dyes enabled staining of these bacteria and reduced their adherence to human T24 bladder carcinoma cells by 48%. The results are of urgent interest to study S. aureus infections.
authors.affiliation: Institute of Organic Chemistry, Julius-Maximilians-University Würzburg, Am Hubland, 97074 Würzburg, Germany.
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Memmel
authors.forename: Arne
authors.initials: A
authors.lastname: Homann
authors.forename: Tobias A
authors.initials: TA
authors.lastname: Oelschlaeger
authors.forename: Jürgen
authors.initials: J
authors.lastname: Seibel
mesh_terms: D018829:Adhesins, Bacterial / Q000737:chemistry / Q000378:metabolism*; D001386:Azides / Q000737:chemistry; D001422:Bacterial Adhesion / Q000502:physiology*; D045744:Cell Line, Tumor; D006801:Humans; D060847:Metabolic Engineering; D011134:Polysaccharides / Q000737:chemistry* / Q000378:metabolism*; D013211:Staphylococcus aureus / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D001743:Urinary Bladder / Q000166:cytology
chemical_list: D018829:Adhesins, Bacterial; D001386:Azides; D011134:Polysaccharides