title: Sulforaphane restores cellular glutathione levels and reduces chronic periodontitis neutrophil hyperactivity in vitro.
abstract: The production of high levels of reactive oxygen species by neutrophils is associated with the local and systemic destructive phenotype found in the chronic inflammatory disease periodontitis. In the present study, we investigated the ability of sulforaphane (SFN) to restore cellular glutathione levels and reduce the hyperactivity of circulating neutrophils associated with chronic periodontitis. Using differentiated HL60 cells as a neutrophil model, here we show that generation of extracellular O2 (. -) by the nicotinamide adenine dinucleotide (NADPH) oxidase complex is increased by intracellular glutathione depletion. This may be attributed to the upregulation of thiol regulated acid sphingomyelinase driven lipid raft formation. Intracellular glutathione was also lower in primary neutrophils from periodontitis patients and, consistent with our previous findings, patients neutrophils were hyper-reactive to stimuli. The activity of nuclear factor erythroid-2-related factor 2 (Nrf2), a master regulator of the antioxidant response, is impaired in circulating neutrophils from chronic periodontitis patients. Although patients' neutrophils exhibit a low reduced glutathione (GSH)/oxidised glutathione (GSSG) ratio and a higher total Nrf2 level, the DNA-binding activity of nuclear Nrf2 remained unchanged relative to healthy controls and had reduced expression of glutamate cysteine ligase catalytic (GCLC), and modifier (GCLM) subunit mRNAs, compared to periodontally healthy subjects neutrophils. Pre-treatment with SFN increased expression of GCLC and GCM, improved intracellular GSH/GSSG ratios and reduced agonist-activated extracellular O2 (. -) production in both dHL60 and primary neutrophils from patients with periodontitis and controls. These findings suggest that a deficiency in Nrf2-dependent pathways may underpin susceptibility to hyper-reactivity in circulating primary neutrophils during chronic periodontitis.
authors.affiliation: Life and Health Sciences, Aston Research Centre for Healthy Ageing, Aston University, Birmingham, United Kingdom.
authors.forename: Irundika H K
authors.initials: IH
authors.lastname: Dias
authors.forename: Ian L C
authors.initials: IL
authors.lastname: Chapple
authors.forename: Mike
authors.initials: M
authors.lastname: Milward
authors.forename: Melissa M
authors.initials: MM
authors.lastname: Grant
authors.forename: Eric
authors.initials: E
authors.lastname: Hill
authors.forename: James
authors.initials: J
authors.lastname: Brown
authors.forename: Helen R
authors.initials: HR
authors.lastname: Griffiths
mesh_terms: D000328:Adult; D002908:Chronic Disease; D005260:Female; D005978:Glutathione / Q000378:metabolism*; D018922:HL-60 Cells; D006801:Humans; D066298:In Vitro Techniques; D017879:Isothiocyanates / Q000494:pharmacology*; D008297:Male; D008875:Middle Aged; D019255:NADPH Oxidases / Q000378:metabolism; D051267:NF-E2-Related Factor 2 / Q000097:blood; D009504:Neutrophils / Q000187:drug effects / Q000276:immunology*; D010518:Periodontitis / Q000276:immunology / Q000378:metabolism*; D016897:Respiratory Burst / Q000187:drug effects; D013454:Sulfoxides; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
chemical_list: D017879:Isothiocyanates; D051267:NF-E2-Related Factor 2; C495635:NFE2L2 protein, human; D013454:Sulfoxides; D019255:NADPH Oxidases; C016766:sulforaphane; D005978:Glutathione; D013755:Tetradecanoylphorbol Acetate