title: Sensitivity of a green alga to atrazine is not enhanced by previous acute exposure.
abstract: Exposure to atrazine in small lotic systems can be episodic, with short-term pulses (peaks) followed by lower, decreasing concentrations. Algae and macrophytes recover rapidly from pulsed exposure to atrazine, but reported observations of population response to subsequent exposures are minimal and inconclusive. Consequently, the sensitivity of Pseudokirchneriella subcapitata to atrazine following a pulsed exposure was assessed. Exposure concentrations reflected amplifications of those observed in streams from highly vulnerable watersheds in regions of intense use. Initial pulsed atrazine exposure at 0, 150 or 300 μg/L for 24-h was followed by 72-h exposure to 0, 5, 10, 25, or 50 μg/L. Measured responses were cell density, growth rate, chlorophyll-a, and maximum quantum yield of photosystem II. Algal recovery was rapid and prior pulsed exposure to atrazine did not significantly affect subsequent sensitivity (EC10s, EC25s) for any endpoint, indicating no changes in tolerance at the population level for this species.
authors.affiliation: School of Environmental Sciences, University of Guelph, Guelph, Ontario, Canada.
authors.forename: Leilan
authors.initials: L
authors.lastname: Baxter
authors.forename: Richard
authors.initials: R
authors.lastname: Brain
authors.forename: Ryan
authors.initials: R
authors.lastname: Prosser
authors.forename: Keith
authors.initials: K
authors.lastname: Solomon
authors.forename: Mark
authors.initials: M
authors.lastname: Hanson
mesh_terms: D001280:Atrazine / Q000633:toxicity*; D002734:Chlorophyll / Q000378:metabolism; D000460:Chlorophyta / Q000502:physiology*; D006540:Herbicides / Q000633:toxicity*; D045332:Photosystem II Protein Complex / Q000378:metabolism; D023382:Toxicity Tests, Acute; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D006540:Herbicides; D045332:Photosystem II Protein Complex; D014874:Water Pollutants, Chemical; D002734:Chlorophyll; D001280:Atrazine
keywords: Algae; Atrazine; Pulsed exposure; Recovery; Sensitivity