title: Effects of pectin pentaoligosaccharide from Hawthorn ( Crataegus pinnatifida Bunge. var. Major) on the activity and mRNA levels of enzymes involved in fatty acid oxidation in the liver of mice fed a high-fat diet.
abstract: The regulatory effects of haw pectin pentaoligosaccharide (HPPS) on fatty acid oxidation-related enzyme activities and mRNA levels were investigated in the liver of high fat diet induced hyperlipidemic mice. Results showed that HPPS (150 mg/kg for 10 weeks) significantly suppresses weight gain (32.3 ± 0.26 and 21.1 ± 0.14 g for high-fat diet and HPPS groups, respectively), decreases serum triacylglycerol levels (1.64 ± 0.09 and 0.91 ± 0.02 mmol/L, respectively), and increases lipid excretion in feces (55.7 ± 0.38 and 106.4 ± 0.57 mg/g for total lipid, respectively), compared to high-fat diet as control. HPPS significantly increased the hepatic fatty acid oxidation-related enzyme activities of acyl-CoA oxidase, carnitine palmitoyltransferase I, 3-ketoacyl-CoA thiolase, and 2,4-dienoyl-CoA reductase by 53.8, 74.2, 47.1, and 24.2%, respectively. Meanwhile, the corresponding mRNAs were up-regulated by 89.6, 85.8, 82.9, and 30.9%, respectively. Moreover, HPPS was able to up-regulate the gene and protein expressions of peroxisome proliferator-activated receptor α. Results suggest that continuous HPPS ingestion may be used as dietary therapy to prevent obesity and cardiovascular diseases.
authors.affiliation: Department of Food Science, Liaoning University ,; The Engineering Technology Research Center for Food Bioprocessing of Liaoning Province; and The Key Laboratory of Food Bioprocessing and Quality Control Technology, Shenyang 110036, China.
authors.forename: Tuo-Ping
authors.initials: TP
authors.lastname: Li
authors.forename: Ru-Gang
authors.initials: RG
authors.lastname: Zhu
authors.forename: Yin-Ping
authors.initials: YP
authors.lastname: Dong
authors.forename: Yong-Hui
authors.initials: YH
authors.lastname: Liu
authors.forename: Su-Hong
authors.initials: SH
authors.lastname: Li
authors.forename: Gang
authors.initials: G
authors.lastname: Chen
mesh_terms: D042962:Acyl-CoA Oxidase / Q000378:metabolism; D000818:Animals; D002334:Carnitine O-Palmitoyltransferase / Q000378:metabolism; D027825:Crataegus / Q000737:chemistry*; D059305:Diet, High-Fat / Q000009:adverse effects; D005227:Fatty Acids / Q000378:metabolism*; D006801:Humans; D006949:Hyperlipidemias / Q000188:drug therapy* / Q000201:enzymology / Q000235:genetics / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000201:enzymology* / Q000378:metabolism; D008297:Male; D051379:Mice; D009844:Oligosaccharides / Q000008:administration & dosage*; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000378:metabolism; D044925:Oxidoreductases Acting on CH-CH Group Donors / Q000378:metabolism; D010368:Pectins / Q000008:administration & dosage*; D010936:Plant Extracts / Q000008:administration & dosage*
chemical_list: D005227:Fatty Acids; D009844:Oligosaccharides; D010936:Plant Extracts; D010368:Pectins; D010088:Oxidoreductases; D044925:Oxidoreductases Acting on CH-CH Group Donors; C100153:2,4-dienoyl-CoA reductase; D042962:Acyl-CoA Oxidase; D002334:Carnitine O-Palmitoyltransferase