title: Reference intervals for red cell variables and platelet counts in infants at 2, 5 and 13 months of age: a cohort study.
abstract: OBJECTIVE
  To derive reference values for red cell variables and platelet counts from a cohort of infants sampled at precise ages during the first 13 months of life.
  
  
  METHODS
  Blood counts, reticulocyte counts and zinc protoporphyrin concentrations were obtained from healthy term infants of North European ancestry at 2, 5 and 13 months of age.
  
  
  RESULTS
  Mean cell volume (MCV) and mean cell haemoglobin (MCH) values did not differ significantly between 5 and 13 months and MCH concentration was unaffected by age. Values of all other variables at any one age differed significantly from those at the other two. Haemoglobin, mean cell haemoglobin, zinc protoporphyrin and platelet values (95% ranges) at 2 (n=119), 5 (n=97) and 13 months (n=42) were, respectively, 91-125, 101-129 and 105-133 g/L; 28.6-33.1, 24.5-28.7 and 24.3-28.7 pg; 36-116, 25-91 and 27-57 micromol/mol haem; and 216-658, 241-591 and 209-455×10(9)/L. At 2 and 5 months, respectively, 26.9% and 10.8% of subjects had platelet counts >500×10(9)/L. Reticulocyte counts at 2 months and MCV and MCH values at 5 months were significantly higher in girls. In boys, red cell distribution width values were significantly higher at 5 months, and zinc protoporphyrin values at both 2 and 5 months.
  
  
  CONCLUSIONS
  These findings indicate the value of obtaining reference data at precise ages during infancy and confirm and extend earlier reports indicating a gender difference in laboratory measures used to assess iron status in early infancy.
authors.affiliation: Department of Paediatric Haematology, Sheffield Children's NHS Foundation Trust, , Sheffield, UK.
authors.forename: R F
authors.initials: RF
authors.lastname: Hinchliffe
authors.forename: G J
authors.initials: GJ
authors.lastname: Bellamy
authors.forename: F
authors.initials: F
authors.lastname: Bell
authors.forename: A
authors.initials: A
authors.lastname: Finn
authors.forename: A J
authors.initials: AJ
authors.lastname: Vora
authors.forename: L
authors.initials: L
authors.lastname: Lennard
mesh_terms: D000367:Age Factors; D001772:Blood Cell Count; D015331:Cohort Studies; D004909:Erythrocyte Indices; D005260:Female; D006400:Hematocrit; D006454:Hemoglobins / Q000032:analysis / Q000378:metabolism; D006801:Humans; D007223:Infant; D007501:Iron / Q000097:blood; D008297:Male; D010976:Platelet Count / Q000592:standards*; D011524:Protoporphyrins / Q000097:blood; D012016:Reference Values; D017701:Reticulocyte Count / Q000592:standards*; D012737:Sex Factors
chemical_list: D006454:Hemoglobins; D011524:Protoporphyrins; C017803:zinc protoporphyrin; D007501:Iron
keywords: ERYTHROCYTE; IRON; PAEDIATRIC HAEMATOLOGY; PLATELETS; RETICULOCYTE COUNTS