title: Optimization of process variables for phyllanthin extraction from Phyllanthus amarus leaves by supercritical fluid using a Box-Behnken experimental design followed by HPLC identification.
abstract: The response surface methodology using the Box-Behnken design was established to describe supercritical carbon dioxide assisted extraction of phyllanthin from Phyllanthus amarus Schum and Thonn leaves prior to HPLC analysis. The effects of extraction pressure, temperature, modifier concentration and extraction time on the yield of phyllanthin were investigated. By solving the regression equation, the optimum conditions were as follows: extraction pressure 23.2 MPa, temperature 40 °C, methanol as modifier at a concentration of 10 % and time 90 min. Under these conditions, the phyllanthin yield was 12.83 ± 0.28 mg g-1, which was in good agreement with the predicted values. Modifier concentration and extraction time showed a significant effect on the phyllanthin yield.
authors.affiliation: Pharmaceutical Technology Division, Department of Chemical Technology, Dr. Babasaheb Ambedkar Marathwada University, Aurangabad, India.
authors.forename: Ajit A
authors.initials: AA
authors.lastname: Patil
authors.forename: Sachin S
authors.initials: SS
authors.lastname: Bhusari
authors.forename: Devanand B
authors.initials: DB
authors.lastname: Shinde
authors.forename: Pravin S
authors.initials: PS
authors.lastname: Wakte
mesh_terms: D002245:Carbon Dioxide / Q000737:chemistry; D005591:Chemical Fractionation / Q000379:methods*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D017705:Lignans / Q000737:chemistry / Q000494:pharmacology; D000432:Methanol / Q000737:chemistry; D029803:Phyllanthus / Q000737:chemistry*; D010936:Plant Extracts; D018515:Plant Leaves; D011312:Pressure; D013696:Temperature; D013997:Time Factors
chemical_list: D017705:Lignans; D010936:Plant Extracts; D002245:Carbon Dioxide; C517985:phyllanthin; D000432:Methanol