title: Identification and characterization of an oleate hydratase-encoding gene from Bifidobacterium breve.
abstract: Bifidobacteria are common commensals of the mammalian gastrointestinal tract. Previous studies have suggested that a bifidobacterial myosin cross reactive antigen (MCRA) protein plays a role in bacterial stress tolerance, while this protein has also been linked to the biosynthesis of conjugated linoleic acid (CLA) in bifidobacteria. In order to increase our understanding on the role of MCRA in bifidobacteria we created and analyzed an insertion mutant of the MCRA-encoding gene of B. breve NCFB 2258. Our results demonstrate that the MCRA protein of B. breve NCFB 2258 does not appear to play a role in CLA production, yet is an oleate hydratase, which contributes to bifidobacterial solvent stress protection.
authors.affiliation: Department of Microbiology; University College Cork; Cork, Ireland; Alimentary Pharmabiotic Centre; University College Cork; Cork, Ireland; Teagasc Research Centre Moorepark; Fermoy; Cork, Ireland; Department of Plant Biochemistry; Georg-August-University; Goettingen, Germany.
authors.forename: Kerry Joan
authors.initials: KJ
authors.lastname: O'Connell
authors.forename: Mary O'Connell
authors.initials: MO
authors.lastname: Motherway
authors.forename: Alan A
authors.initials: AA
authors.lastname: Hennessey
authors.forename: Florian
authors.initials: F
authors.lastname: Brodhun
authors.forename: R Paul
authors.initials: RP
authors.lastname: Ross
authors.forename: Ivo
authors.initials: I
authors.lastname: Feussner
authors.forename: Catherine
authors.initials: C
authors.lastname: Stanton
authors.forename: Gerald F
authors.initials: GF
authors.lastname: Fitzgerald
authors.forename: Douwe
authors.initials: D
authors.lastname: van Sinderen
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D001644:Bifidobacterium / Q000201:enzymology / Q000235:genetics*; D015870:Gene Expression; D006836:Hydro-Lyases / Q000235:genetics / Q000378:metabolism*; D044243:Linoleic Acids, Conjugated / Q000378:metabolism; D016254:Mutagenesis, Insertional; D009829:Oleic Acids / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D044243:Linoleic Acids, Conjugated; D009829:Oleic Acids; D006836:Hydro-Lyases
keywords: bifidobacteria; conjugated linoleic acid; hydratase; myosin cross reactive antigen; probiotic