title: MicroRNA-128 promotes cell-cell adhesion in U87 glioma cells via regulation of EphB2.
abstract: MicroRNAs (miRNAs) are small, non-coding RNAs which regulate gene expression at the post-transcriptional level. Abnormal expression of miRNAs occurs frequently in human tumors. Despite the fact that reduced expression of miR-128 has been observed in glioma tissues and cells, the role of miR-128 in tumors has not been fully characterized. In the present study, cell adhesion assays indicated that overexpression of miR-128 can promote cell-cell adhesion. Target site prediction algorithms indicated that miR-128 binds the 3'-untranslated regions of erythropoietin-producing hepatocellular receptor (Eph)B1 and EphB2 mRNAs. Luciferase reporter assays confirmed that miR-128 binds and regulates EphB1 and EphB2 mRNAs. Overexpression of EphB2 reduced the ability of miR-128 to promote cell-cell adhesion. The wound-healing assay indicated that miR-128 significantly inhibited cell migration via EphB2. This study revealed the novel functions of miR-128 in cell-cell adhesion and cell migration in glioma cells through the regulation of EphB2, and identified EphB1 and EphB2 as novel miR-128 targets.
authors.affiliation: Key Laboratory of Gene Engineering of the Ministry of Education, State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, P.R. China.
authors.forename: Lina
authors.initials: L
authors.lastname: Lin
authors.forename: Xulin
authors.initials: X
authors.lastname: Chen
authors.forename: Xuelin
authors.initials: X
authors.lastname: Peng
authors.forename: Jianwen
authors.initials: J
authors.lastname: Zhou
authors.forename: Hsiang-Fu
authors.initials: HF
authors.lastname: Kung
authors.forename: Marie C
authors.initials: MC
authors.lastname: Lin
authors.forename: Songshan
authors.initials: S
authors.lastname: Jiang
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics*; D015153:Blotting, Western; D001932:Brain Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D002448:Cell Adhesion; D002465:Cell Movement; D049109:Cell Proliferation; D015972:Gene Expression Regulation, Neoplastic; D005910:Glioma / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D006801:Humans; D016086:Lentivirus / Q000235:genetics; D008156:Luciferases / Q000378:metabolism; D035683:MicroRNAs / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D036183:Receptor, EphB2 / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014407:Tumor Cells, Cultured; D014945:Wound Healing
chemical_list: D020413:3' Untranslated Regions; C545189:MIRN128 microRNA, human; D035683:MicroRNAs; D012333:RNA, Messenger; D008156:Luciferases; C586310:EPHB2 protein, human; D036183:Receptor, EphB2