title: What is the difference in organic matrix of aragonite vs. vaterite polymorph in natural shell and pearl? Study of the pearl-forming freshwater bivalve mollusc Hyriopsis cumingii.
abstract: Aragonite pearl, vaterite pearl and shell nacre of the freshwater mollusc Hyriopsis cumingii (Zhejiang province, China) were chosen to analyze microstructure and organic composition in the different habits of calcium carbonate. SEM and TEM were used to reveal the microstructure and mineralogical phase. We found that tablets in vaterite exhibited more irregular texture and were packaged with more organic matrices than in aragonite forms. Then a peculiar method was introduced to extract water soluble matrix (WSM), acid soluble matrix (ASM) and acid insoluble matrix (AIM) from the three samples, and biochemical analysis of these organic matrixes involved in crystal formation and polymorph selection was carried out. High performance liquid chromatography (HPLC) confirms the hydrophobic pattern of the organic matrix intermingled with mineral, the opposite of the early mobilizable water soluble fraction. Amino acid composition confirms hydrophobic residues as major components of all the extracts, but it reveals an imbalance in acidic residues rates in WSM vs. ASM and in aragonite vs. vaterite. Electrophoresis gives evidence for signatures in proteins with a 140 kDa material specific for aragonite in WSM. Conversely all ASM extracts reveal the presence of about 55 kDa components, including a discrete band in vaterite extract.
authors.affiliation: State Key Laboratory of New Ceramics and Fine Processing, Department of Materials Science and Engineering, Tsinghua University, Beijing 100084, China.
authors.forename: Yufei
authors.initials: Y
authors.lastname: Ma
authors.forename: Sophie
authors.initials: S
authors.lastname: Berland
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Andrieu
authors.forename: Qingling
authors.initials: Q
authors.lastname: Feng
authors.forename: Laurent
authors.initials: L
authors.lastname: Bédouet
mesh_terms: D000596:Amino Acids / Q000032:analysis; D060105:Animal Shells / Q000033:anatomy & histology / Q000737:chemistry*; D000818:Animals; D049872:Bivalvia / Q000737:chemistry*; D002119:Calcium Carbonate / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D004586:Electrophoresis; D005618:Fresh Water; D008903:Minerals / Q000737:chemistry; D060734:Nacre / Q000737:chemistry*; D009930:Organic Chemicals / Q000032:analysis*; D011506:Proteins / Q000032:analysis; D012995:Solubility; D014867:Water / Q000737:chemistry
chemical_list: D000596:Amino Acids; D008903:Minerals; D060734:Nacre; D009930:Organic Chemicals; D011506:Proteins; D014867:Water; D002119:Calcium Carbonate
keywords: Aragonite; Biochemistry analysis; Hyriopsis cumingii; Organic matrix; Vaterite