title: An efficient single prime protocol for the induction of antigen-induced airways inflammation.
abstract: There are a number of mouse models of allergic airway inflammation used to delineate various aspects of asthma. One of the hurdles with using mice is their natural resistance to developing a Th2 allergic response. This is often overcome by double priming with the allergen and an adjuvant. Here we report on an efficient 11day, single antigen/alum priming protocol that is sufficient to sensitise mice for the development of Th2-mediated inflammation in the lung following antigen challenge.
authors.affiliation: Inflammation and Arthritis Group, Malaghan Institute of Medical Research, Wellington, New Zealand.
authors.forename: Odette M
authors.initials: OM
authors.lastname: Shaw
authors.forename: Jacquie L
authors.initials: JL
authors.lastname: Harper
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage; D000534:Alum Compounds / Q000008:administration & dosage; D000818:Animals; D000941:Antigens / Q000009:adverse effects; D001249:Asthma / Q000209:etiology* / Q000276:immunology / Q000473:pathology; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology / Q000276:immunology; D004195:Disease Models, Animal; D007073:Immunoglobulin E / Q000097:blood; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D010047:Ovalbumin / Q000008:administration & dosage / Q000276:immunology; D018418:Th2 Cells / Q000276:immunology; D013997:Time Factors
chemical_list: D000276:Adjuvants, Immunologic; D000534:Alum Compounds; D000941:Antigens; C041524:aluminum sulfate; D007073:Immunoglobulin E; D010047:Ovalbumin
keywords: Allergic airways inflammation; Antigen sensitization; Ovalbumin; Single prime; Th2 response