title: The detection of enteroviruses in sewage using Caco-2 cells.
abstract: The work presented here demonstrates the utility of Caco-2 cells to detect enteroviruses in sewage. Viruses were concentrated by beef extract elution and organic flocculation prior to analysis by cell culture assays and RT-PCR. Enteroviruses were detected in all sewage samples, but only one sample was positive solely in RT-PCR assay. We proved that Caco-2 cells were more effective than RD and L20B cells in enterovirus isolation, depending on procedures used in the inoculation process.
authors.affiliation: National Institute of Public Health - National Institute of Hygiene Department of Virology, Warsaw, Poland. mrechnio@pzh.gov.pl
authors.forename: Magdalena
authors.initials: M
authors.lastname: Wieczorek
authors.forename: Łukasz
authors.initials: Ł
authors.lastname: Kuryk
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Witek
authors.forename: Anna
authors.initials: A
authors.lastname: Diuwe
authors.forename: Bogumiła
authors.initials: B
authors.lastname: Litwińska
mesh_terms: D018938:Caco-2 Cells; D004770:Enterovirus / Q000302:isolation & purification* / Q000502:physiology; D006801:Humans; D012722:Sewage / Q000821:virology*; D014776:Virus Cultivation / Q000379:methods*
chemical_list: D012722:Sewage