title: Insights from the molecular characterization of mercury stress proteins identified by proteomics in E.coli nissle 1917.
abstract: Differently expressed proteins in probiotic Escherichia coli nissle 1917 under mercury stress identified by using a proteomic approach. We applied to separate proteins by using two-dimensional gel electrophoresis and proteins were identified using MALDI-TOF-MS using PMF, by mascot database search using the NCBI database. we identified six proteins after exposure to mercury stress with respect to different functional classes. It is useful to understand the molecular insights into mercury stress in probiotic E. coli. Next we describe a structure generated by homology modelling and functional domain identification; it is interesting to study the impact of stress on protein structures. MS characterization and computational methods together provide the opportunity to examine the impact of stress arising from mercury. The role of these proteins in metal tolerance and structure relation is discussed. To the best of our knowledge, proteomics of E. coli nissle 1917 overview of mercury stress has been reported for the first time.
authors.affiliation: Department of Microbiology, Sri Venkateswara University, Tirupati-517 502, A.P.
authors.forename: Panthangi
authors.initials: P
authors.lastname: Seshapani
authors.forename: Daddam Jayasimha
authors.initials: DJ
authors.lastname: Rayalu
authors.forename: Vadde Kiran
authors.initials: VK
authors.lastname: Kumar
authors.forename: Kathera Chandra
authors.initials: KC
authors.lastname: Sekhar
authors.forename: Jasti Pramoda
authors.initials: JP
authors.lastname: Kumari
keywords: 2D-PAGE; E. coli nissle 1917; Homology modelling; Mass spectroscopy; Mercury