title: BCL2A1 is a potential biomarker for postoperative seizure control in patients with low-grade gliomas.
abstract: OBJECTIVE
  To identify molecular genetic factors that influence preoperative seizure occurrence and postoperative seizure control in patients with low-grade gliomas (LGGs).
  
  
  METHODS
  Fifty-four WHO grade II astrocytomas were used for microarray analysis under strict inclusion criteria. The primary endpoint was seizure control at 12 months after surgery. Biological processes were investigated by gene ontology (GO) analysis. Quantitative RT-PCR and immunohistochemistry were used to validate key genes.
  
  
  RESULTS
  Differentially expressed genes correlated with seizure occurrence failed to significantly distinguish patients with and without a history of seizures. With respect to postoperative seizure control, a transcript profile of 92 genes was identified, which successfully separated patients with good and poor seizure prognosis. GO analysis revealed that the most striking overrepresentation of genes was found in a category of anti-apoptotic genes and their regulation. Increased expression was also observed for genes involved in immune and inflammatory responses. BCL2A1 was proven to be a novel marker associated with seizure prognosis.
  
  
  CONCLUSIONS
  Increased anti-apoptotic activity of tumor cells appears to contribute to seizure recurrence after surgery in patients with LGGs. These findings provide insights that may lead to the development of effective treatment strategies for prolonging the survival of patients with LGG in the future.
authors.affiliation: Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing, China.
authors.forename: Gan
authors.initials: G
authors.lastname: You
authors.forename: Lin
authors.initials: L
authors.lastname: Feng
authors.forename: Wei
authors.initials: W
authors.lastname: Yan
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: Yong-Zhi
authors.initials: YZ
authors.lastname: Wang
authors.forename: Shao-Wu
authors.initials: SW
authors.lastname: Li
authors.forename: Shou-Wei
authors.initials: SW
authors.lastname: Li
authors.forename: Gui-Lin
authors.initials: GL
authors.lastname: Li
authors.forename: Yi-Jun
authors.initials: YJ
authors.lastname: Song
authors.forename: Chun-Sheng
authors.initials: CS
authors.lastname: Kang
authors.forename: Yong-Ping
authors.initials: YP
authors.lastname: You
authors.forename: Tao
authors.initials: T
authors.lastname: Jiang
mesh_terms: D000293:Adolescent; D000328:Adult; D014408:Biomarkers, Tumor / Q000096:biosynthesis / Q000235:genetics*; D001932:Brain Neoplasms / Q000175:diagnosis / Q000235:genetics* / Q000601:surgery; D005260:Female; D005910:Glioma / Q000175:diagnosis / Q000235:genetics* / Q000601:surgery*; D006801:Humans; D008297:Male; D008875:Middle Aged; D015778:Minor Histocompatibility Antigens; D011183:Postoperative Complications / Q000175:diagnosis / Q000235:genetics* / Q000517:prevention & control; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000096:biosynthesis / Q000235:genetics*; D012008:Recurrence; D012640:Seizures / Q000175:diagnosis / Q000235:genetics* / Q000517:prevention & control; D055815:Young Adult
chemical_list: C096816:BCL2-related protein A1; D014408:Biomarkers, Tumor; D015778:Minor Histocompatibility Antigens; D019253:Proto-Oncogene Proteins c-bcl-2
keywords: BCL2A1; Epilepsy; Glioma; Prognosis; Seizure