title: The Ecdysone receptor constrains wingless expression to pattern cell cycle across the Drosophila wing margin in a Cyclin B-dependent manner.
abstract: BACKGROUND
  Ecdysone triggers transcriptional changes via the ecdysone receptor (EcR) to coordinate developmental programs of apoptosis, cell cycle and differentiation. Data suggests EcR affects cell cycle gene expression indirectly and here we identify Wingless as an intermediary factor linking EcR to cell cycle.
  
  
  RESULTS
  We demonstrate EcR patterns cell cycle across the presumptive Drosophila wing margin by constraining wg transcription to modulate CycB expression, but not the previously identified Wg-targets dMyc or Stg. Furthermore co-knockdown of Wg restores CycB patterning in EcR knockdown clones. Wg is not a direct target of EcR, rather we demonstrate that repression of Wg by EcR is likely mediated by direct interaction between the EcR-responsive zinc finger transcription factor Crol and the wg promoter.
  
  
  CONCLUSIONS
  Thus we elucidate a critical mechanism potentially connecting ecdysone with patterning signals to ensure correct timing of cell cycle exit and differentiation during margin wing development.
authors.affiliation: Department of Anatomy and Cell Biology, University of Melbourne, Parkville 3010, Melbourne, Australia.
authors.forename: Naomi C
authors.initials: NC
authors.lastname: Mitchell
authors.forename: Jane I
authors.initials: JI
authors.lastname: Lin
authors.forename: Olga
authors.initials: O
authors.lastname: Zaytseva
authors.forename: Nicola
authors.initials: N
authors.lastname: Cranna
authors.forename: Amanda
authors.initials: A
authors.lastname: Lee
authors.forename: Leonie M
authors.initials: LM
authors.lastname: Quinn
mesh_terms: D000818:Animals; D002453:Cell Cycle; D019926:Cyclin B / Q000378:metabolism*; D004330:Drosophila / Q000378:metabolism*; D029721:Drosophila Proteins / Q000235:genetics*; D011987:Receptors, Steroid / Q000378:metabolism* / Q000502:physiology*; D014921:Wings, Animal / Q000378:metabolism; D051155:Wnt1 Protein / Q000235:genetics*
chemical_list: D019926:Cyclin B; D029721:Drosophila Proteins; D011987:Receptors, Steroid; D051155:Wnt1 Protein; C055469:ecdysone receptor; C074622:wg protein, Drosophila