title: A rapid and economical method for low molecular weight RNA isolation from a wide variety of plant species.
abstract: We have developed a time- and cost-effective method for isolating low molecular weight (LMW) RNA from plants. In our protocol, the isolation procedure can be completed within 3 h. Polyethylene glycol (PEG) and absolute ethanol are used to isolate LMW RNA, and the LMW RNA yields were >80 µg/g of fresh-weight tissues for several of the plant species tested.
authors.affiliation: College of Agriculture, Hainan University, Haikou, Hainan 570228, Japan.
authors.forename: Zewei
authors.initials: Z
authors.lastname: An
authors.forename: Yachao
authors.initials: Y
authors.lastname: Li
authors.forename: Lili
authors.initials: L
authors.lastname: Xie
authors.forename: Qilin
authors.initials: Q
authors.lastname: Zhai
authors.forename: Huasun
authors.initials: H
authors.lastname: Huang
mesh_terms: D001483:Base Sequence; D005591:Chemical Fractionation / Q000379:methods*; D008970:Molecular Weight; D010944:Plants / Q000235:genetics*; D018749:RNA, Plant / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification*; D013997:Time Factors
chemical_list: D018749:RNA, Plant