title: The structure of the TOG-like domain of Drosophila melanogaster Mast/Orbit.
abstract: Mast/Orbit is a nonmotor microtubule-associated protein (MAP) present in Drosophila melanogaster that reportedly binds microtubules at the plus end and is essential for mitosis. Sequence analysis has shown that the N-terminal domain (Mast-M1) resembles TOG domains from the Dis1-TOG family of proteins and stands as a representative of one of the three subclasses of divergent TOG-like domains (TOGL1) that includes human CLASP1. The crystal structure of Mast-M1 has been determined at 2.0 Å resolution and provides the first detailed structural description of any TOG-like domain. The structure confirms that Mast-M1 adopts a similar fold to the previously described Dis1-TOG domains of microtubule-binding proteins. A comparison with three known TOG-domain structures from XMAP215/Dis1 family members exposes significant differences between Mast-M1 and other TOG-domain structures in key residues at the proposed tubulin-binding edge.
authors.affiliation: Department of Medicinal Chemistry, University of Minnesota, 308 Harvard Street SE, 8-101 Weaver-Densford Hall, Minneapolis, MN 55455, USA.
authors.forename: Teresa
authors.initials: T
authors.lastname: De la Mora-Rey
authors.forename: Brian D
authors.initials: BD
authors.lastname: Guenther
authors.forename: Barry C
authors.initials: BC
authors.lastname: Finzel
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D019295:Computational Biology; D003460:Crystallization; D018360:Crystallography, X-Ray; D029721:Drosophila Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D004331:Drosophila melanogaster / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008869:Microtubule-Associated Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D008870:Microtubules / Q000378:metabolism; D008958:Models, Molecular; D008969:Molecular Sequence Data; D011487:Protein Conformation; D017434:Protein Structure, Tertiary; D017386:Sequence Homology, Amino Acid
chemical_list: C497850:CLASP1 protein, human; D029721:Drosophila Proteins; D008869:Microtubule-Associated Proteins; C407307:chb protein, Drosophila
keywords: CLASP; Dis1-TOG domain; TOG-like domains; XMAP215/Dis1; microtubules