title: Development of a double sandwich fluorescent ELISA to detect rattlesnake venom in biological samples from horses with a clinical diagnosis of rattlesnake bite.
abstract: Rattlesnake bites in horses are not uncommon and the clinical outcomes are widely variable. Treatment of horses with anti-venom is often cost prohibitive and could have negative consequences; therefore, the development of a quantitative test to determine if anti-venom therapy is indicated would be valuable. The objective of this study was to develop an ELISA to detect rattlesnake venom in biological samples from clinically bitten horses. Nineteen horses were enrolled in the study. Urine was available from 19 horses and bite site samples were available from 9 horses. A double sandwich fluorescent ELISA was developed and venom was detected in 5 of 9 bite site samples and 12 of 19 urine samples. In order to determine if this assay is useful as a guide for treatment, a correlation between venom concentration and clinical outcome needs to be established. For this, first peak venom concentration needs to be determined. More frequent, consistent sample collection will be required to define a venom elimination pattern in horses and determine the ideal sample collection time to best estimate the maximum venom dose. This report describes development of an assay with the ability to detect rattlesnake venom in the urine and at the bite site of horses with a clinical diagnosis of rattlesnake bite.
authors.affiliation: 1 Farm Rd-OSU BVMTH, Department of Veterinary Clinical Sciences, Center for Veterinary Health Sciences, Oklahoma State University, Stillwater, OK 74078, USA. l.gilliam@okstate.edu
authors.forename: Lyndi L
authors.initials: LL
authors.lastname: Gilliam
authors.forename: Charlotte L
authors.initials: CL
authors.lastname: Ownby
authors.forename: Dianne
authors.initials: D
authors.lastname: McFarlane
authors.forename: Amy
authors.initials: A
authors.lastname: Canida
authors.forename: Todd C
authors.initials: TC
authors.lastname: Holbrook
authors.forename: Mark E
authors.initials: ME
authors.lastname: Payton
authors.forename: Clinton R
authors.initials: CR
authors.lastname: Krehbiel
mesh_terms: D000818:Animals; D003435:Crotalid Venoms / Q000302:isolation & purification* / Q000652:urine; D017839:Crotalus; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005453:Fluorescence; D006734:Horse Diseases / Q000175:diagnosis* / Q000652:urine*; D006736:Horses; D012909:Snake Bites / Q000175:diagnosis / Q000652:urine / Q000662:veterinary*
chemical_list: D003435:Crotalid Venoms
keywords: Fluorescent ELISA; Horse; Rattlesnake; Venom