title: [Effects of recombinant human osteoprotegerin and recombinant RANK protein on the differentiation of osteoclast precursors].
abstract: OBJECTIVE
  To compare the effect of recombinant OPG-Fc and recombinant RANK protein on the differentiation of osteoclast precursors.
  
  
  METHODS
  Mouse osteoblasts cell lines were incubated with osteoclast precursors cell lines RAW 264.7 for 9 days with 10(-5) g/L rhRANK or rhOPG-Fc or PBS added to the coculture system. TRAP stain positive cells counting and cortical bone pit formation counting were performed in the 9th day.
  
  
  RESULTS
  Multinuleated TRAP stain positive cells were observed in the cocluture systems after 6 days incubation,and plenty of mature osteoclasts could be observed in the 9th day. With the addition of 10(-5) g/L rhOPG-Fc or rhRANK, multinucleated giant cells and cortical bone pit formation couting decreased significantly compared with the control group, and the rhRANK group decreased more significantly than the rhOPG-Fc group.
  
  
  CONCLUSIONS
  Both rhOPG-Fc and rhRANK can inhabit the differentiation of osteoclast precursors and prevent them forming mature osteoclasts,moreover,the rhRANK shows the significant inhabition effect than the rhOPG-Fc.
authors.affiliation: Department of Orthopaedics, General Hospital of Chinese PLA, Beijing, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Xiong
authors.forename: Li-Cheng
authors.initials: LC
authors.lastname: Zhang
authors.forename: Li-Hai
authors.initials: LH
authors.lastname: Zhang
authors.forename: Qi
authors.initials: Q
authors.lastname: Yao
authors.forename: Peifu
authors.initials: P
authors.lastname: Tang
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D051379:Mice; D010010:Osteoclasts / Q000166:cytology / Q000187:drug effects*; D053244:Osteoprotegerin / Q000494:pharmacology*; D053246:Receptor Activator of Nuclear Factor-kappa B / Q000494:pharmacology*; D011994:Recombinant Proteins / Q000494:pharmacology; D013234:Stem Cells / Q000166:cytology / Q000187:drug effects*
chemical_list: D053244:Osteoprotegerin; D053246:Receptor Activator of Nuclear Factor-kappa B; D011994:Recombinant Proteins