title: Histomorphometric and immunohistochemical study of the goat reticulum during prenatal development.
abstract: This study sought to describe the morphological changes taking place in the goat reticulum during prenatal development, using histomorphometric and immunohistochemical techniques. A total of 140 goat embryos and foetuses were used, from the first stages of prenatal life until birth. Differentiation of the reticulum as a separate compartment of the primitive gastric tube was observed at 35 days of prenatal life (23% gestation). By 38 days (25% gestation) the reticular wall comprised three layers: an internal epithelial layer, a middle layer of pluripotential blastemic tissue and an external layer or serosa. Primary reticular crests were visible at 59 days (38% gestation) as evaginations of the epithelial stratum basale, marking the earliest histological differentiation of future reticular cells. Secondary reticular crests were observed at 87 days (61% gestation). Corneum papillae first became apparent on the lateral surface of primary reticular crests at 101 days (64% gestation). The muscularis mucosae was visible by 101 days (64% gestation) in primary reticular crests. Neuroendocrine cells were detected by synaptophysin at 64 days (43% gestation), while glial cell markers (glial fibrillary acidic protein and vimentin) were observed at 64 days (43% gestation) and 38 days (25% gestation), respectively. The peptidergic innervation markers such as neuropeptide Y and vasoactive intestinal polypeptide were detected at 75 days (50% gestation). In conclusion, prenatal development of the reticulum - like that of the rumen - appears to take place somewhat earlier in goats than in sheep or cattle, but at a similar rate to that reported in deer.
authors.affiliation: Department of Veterinary Histology, Faculty of Veterinary Medicine, University of Extremadura, Cáceres, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Garcia
authors.forename: Javier
authors.initials: J
authors.lastname: Masot
authors.forename: Antonio
authors.initials: A
authors.lastname: Franco
authors.forename: Antonio
authors.initials: A
authors.lastname: Gazquez
authors.forename: E
authors.initials: E
authors.lastname: Redondo
mesh_terms: D000818:Animals; D015509:Developmental Biology; D004622:Embryo, Mammalian / Q000502:physiology* / Q000648:ultrastructure; D005260:Female; D047109:Fetal Development / Q000502:physiology*; D005333:Fetus / Q000502:physiology* / Q000648:ultrastructure; D018507:Gene Expression Regulation, Developmental; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D006041:Goats; D007150:Immunohistochemistry; D008855:Microscopy, Electron, Scanning; D009478:Neuropeptide Y / Q000378:metabolism; D011247:Pregnancy; D011270:Pregnancy, Animal; D012159:Reticulum / Q000196:embryology* / Q000648:ultrastructure*; D016708:Synaptophysin / Q000378:metabolism; D013997:Time Factors; D014660:Vasoactive Intestinal Peptide / Q000378:metabolism; D014746:Vimentin / Q000378:metabolism
chemical_list: D005904:Glial Fibrillary Acidic Protein; D009478:Neuropeptide Y; D016708:Synaptophysin; D014746:Vimentin; D014660:Vasoactive Intestinal Peptide