title: Chemical imaging of lignocellulosic biomass by CARS microscopy.
abstract: Chemical and structural composition of wood biomass is studied by label-free and chemically specific Coherent Anti-Stokes Raman Scattering (CARS) microscopy. A concept developed for assignment and semi-quantitative imaging of sample components; cellulose, hemicellulose, and lignin; by multiplex CARS microspectroscopy and subsequent data analysis is presented. Specific imaging without fluorescence backround is achieved an order of magnitude faster compared with conventional Raman microscopy. Laser polarization control yield information on molecular arrangement in wood fibers. Narrowband CARS excitation of single vibrations allows for three-dimensional volume imaging. Thus, CARS microscopy has potential as an important instrument for characterization of lignocellulosic materials.
authors.affiliation: Physikalisch-Chemisches Institut, Ruprecht-Karls-Universität, Im Neuenheimer Feld 229, 69120 Heidelberg, Germany.
authors.forename: Christoph
authors.initials: C
authors.lastname: Pohling
authors.forename: Christian
authors.initials: C
authors.lastname: Brackmann
authors.forename: Alex
authors.initials: A
authors.lastname: Duarte
authors.forename: Tiago
authors.initials: T
authors.lastname: Buckup
authors.forename: Annika
authors.initials: A
authors.lastname: Enejder
authors.forename: Marcus
authors.initials: M
authors.lastname: Motzkus
mesh_terms: D018533:Biomass; D021621:Imaging, Three-Dimensional; D008031:Lignin / Q000737:chemistry*; D008853:Microscopy / Q000379:methods*; D013059:Spectrum Analysis, Raman / Q000379:methods*; D013997:Time Factors; D014934:Wood / Q000737:chemistry
chemical_list: C036909:lignocellulose; D008031:Lignin
keywords: CARS microscopy; biomass; cellulose; lignin; wood; xylan