title: Seasonal and spatial variability of virioplanktonic abundance in Haihe River, China.
abstract: In order to understand the composition and dynamics of planktonic viruses and their relationship with environmental parameters in natural freshwater, flow cytometry was optimized with filtration/fixation/staining/dilution and then applied to the analysis of samples collected from 9 stations (covering urban, rural, and estuarial areas) along the Haihe River, China, over a one-year period of study. The total viral abundance exhibited an apparent peak in the spring. Spatially, the highest viral abundance was recorded in estuarial areas. The correlation analysis indicated that the bacteria in the Haihe River significantly influenced viral abundance. The relationship between abiotic variables and viral abundance remained the same as with bacterial abundance, indicating that environmental parameters could possibly influence viral abundance in virtue of their bacterial host cells. The influence of environmental factors on viral abundance differed in the three sampling areas, suggesting different drivers of viral abundance in different stretches of the river associated with their utilization and surroundings.
authors.affiliation: Key Laboratory of Pollution Processes and Environmental Criteria (Ministry of Education), Tianjin Key Laboratory of Environmental Remediation and Pollution Control, College of Environmental Science and Engineering, Nankai University, Tianjin 300071, China.
authors.forename: Lili
authors.initials: L
authors.lastname: Ma
authors.forename: Rui
authors.initials: R
authors.lastname: Sun
authors.forename: Guannan
authors.initials: G
authors.lastname: Mao
authors.forename: Hui
authors.initials: H
authors.lastname: Yu
authors.forename: Yingying
authors.initials: Y
authors.lastname: Wang
mesh_terms: D002681:China; D005374:Filtration; D005434:Flow Cytometry; D005843:Geography; D057230:Limit of Detection; D008856:Microscopy, Fluorescence; D010933:Plankton / Q000382:microbiology / Q000821:virology*; D045483:Rivers / Q000382:microbiology*; D012621:Seasons