title: Identification and differential expression of two dehydrin cDNAs during maturation of Jatropha curcas seeds.
abstract: Plant dehydrin proteins (DHNs) are known to be important for environmental stress tolerance and are involved in various developmental processes. Two full-length cDNAs JcDHN-1 and JcDHN-2 encoding two dehydrins from Jatropha curcas seeds were identified and characterized. JcDHN-1 is 764 bp long and contains an open reading frame of 528 bp. The deduced JcDHN-1 protein has 175 a.a. residues that form a 19.3-kDa polypeptide with a predicted isoelectric point (pI) of 6.41. JcDHN-2 is 855 bp long and contains an open reading frame of 441 bp. The deduced JcDHN-2 protein has 156 a.a. residues that form a 17.1-kDa polypeptide with a predicted pI of 7.09. JcDHN-1 is classified as type Y₃SK₂ and JcDHN-2 is classified as type Y₂SK₂ according to the YSK shorthand for structural classification of dehydrins. Homology analysis indicates that both JcDHN-1 and JcDHN-2 share identity with DHNs of other plants. Analysis of the conserved domain revealed that JcDHN-2 has glycoside hydrolase GH20 super-family activity. Quantitative real time PCR analysis for JcDHN-1 and JcDHN-2 expression during seed development showed increasing gene expression of both their transcript levels along with the natural dehydration process during seed development. A sharp increase in JcDHN-2 transcript level occurred in response to water content dropping from 42% in mature seeds to 12% in dry seeds. These results indicate that both JcDHNs have the potential to play a role in cell protection during dehydration occurring naturally during jatropha orthodox seed development.
authors.affiliation: Key Laboratory of Tropical Plant Resource and Sustainable Use, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Menglun 666303, China.
authors.forename: S A
authors.initials: SA
authors.lastname: Omar
authors.forename: N I
authors.initials: NI
authors.lastname: Elsheery
authors.forename: H M
authors.initials: HM
authors.lastname: Kalaji
authors.forename: M K H
authors.initials: MK
authors.lastname: Ebrahim
authors.forename: S
authors.initials: S
authors.lastname: Pietkiewicz
authors.forename: C-H
authors.initials: CH
authors.lastname: Lee
authors.forename: S I
authors.initials: SI
authors.lastname: Allakhverdiev
authors.forename: Zeng-Fu
authors.initials: ZF
authors.lastname: Xu
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D031286:Jatropha / Q000145:classification / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D012639:Seeds / Q000145:classification / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D016415:Sequence Alignment
chemical_list: D018076:DNA, Complementary; D010940:Plant Proteins; C070556:dehydrin proteins, plant