title: Effect of actin polymerization inhibitor during oocyte maturation on parthenogenetic embryo development and ploidy in Capra hircus.
abstract: This study was designed to observe the effect of cytochalasin B (CCB) concentrations on ploidy and early development of parthenogenetic embryos in a caprine species. Caprine oocytes were matured in the presence of different concentrations of CCB (5, 10, 15, and 20 μg/ml) and activated by 7% ethanol followed by incubation with 2 mM DMAP. For embryos fertilized in vitro, oocytes were matured in maturation medium without CCB. The cleavage rate and further embryo development were significantly higher (P < 0.05) when oocytes were treated in this way. The percentage of embryos showed higher diploid values in 15 μg/ml CCB (83.66 ± 1.13), followed by 20 (72.22 ± 1.22), 10 (68.57 ± 1.17), and 5 μg/ml (62.00 ± 2.48). These results indicate that CCB with a concentration of 15 μg/ml in maturation medium can be used for the production of diploid parthenogenetic embryos in the caprine species.
authors.affiliation: Reproductive Physiology and Embryo Transfer Technology Laboratory, Physiology and Climatology Division, Indian Veterinary Research Institute, Izatnagar, Bareilly, 243 122, Uttar Pradesh, India.
authors.forename: R
authors.initials: R
authors.lastname: Ranjan
authors.forename: R K
authors.initials: RK
authors.lastname: Singh
authors.forename: T
authors.initials: T
authors.lastname: Yasotha
authors.forename: Manish
authors.initials: M
authors.lastname: Kumar
authors.forename: Gopal
authors.initials: G
authors.lastname: Puri
authors.forename: Kuldeep
authors.initials: K
authors.lastname: Kumar
authors.forename: Renu
authors.initials: R
authors.lastname: Singh
authors.forename: Sanjeev
authors.initials: S
authors.lastname: Bhure
authors.forename: D
authors.initials: D
authors.lastname: Malakar
authors.forename: S K
authors.initials: SK
authors.lastname: Bhanja
authors.forename: M
authors.initials: M
authors.lastname: Sarkar
authors.forename: B C
authors.initials: BC
authors.lastname: Das
authors.forename: Sadhan
authors.initials: S
authors.lastname: Bag
mesh_terms: D000199:Actins / Q000378:metabolism; D000818:Animals; D003571:Cytochalasin B / Q000494:pharmacology*; D047108:Embryonic Development / Q000187:drug effects* / Q000235:genetics; D005260:Female; D005307:Fertilization in Vitro; D006041:Goats / Q000196:embryology* / Q000235:genetics*; D059785:Karyotype; D009865:Oocytes / Q000187:drug effects* / Q000254:growth & development; D010312:Parthenogenesis / Q000235:genetics; D011003:Ploidies
chemical_list: D000199:Actins; D003571:Cytochalasin B