title: [Advanced oxidation protein products promote expression of stromal-cell derived factor-1alpha of ECV304 cells through ERK signal pathway].
abstract: OBJECTIVE
  To explore the effects of advanced oxidation protein products (AOPP) on expressions of stromal cell-derived factor-1alpha (SDF-1alpha) in ECV304 cells and the signal pathway that mediated the effects.
  
  
  METHODS
  AOPP-BSA was made from bovine serum albumin (BSA) and sodium hypochlorite. After treated with AOPP-BSA of different concentrations (50, 100, 200 micromol/L), the expressions of SDF-1alpha mRNA in ECV304 cells were measured by reverse transcription-polymerase chain reaction (RT-PCR) and the expressions of SDF-1alpha protein and the levels of phosphorylated extracellular signal-regulated kinase (ERK) in ECV304 cells were analyzed by Western blot. In inhibition test, U0126, the special inhibitor of ERK of different concentrations (0.1, 1, 10 rmol/L) were added into ECV304 cells culture media for 1 hour, then the cells were treated with AOPP-BSA for 24 hours, at last the protein levels in supernatant were detected by enzyme-linked immunosorbent assay (ELISA).
  
  
  RESULTS
  AOPP-BSA obviously promoted the expressions of SDF-1alpha mRNA and increased the levels of SDF-1beta protein of ECV304 cells in dose-dependent manner (all P < 0.01), after 15 minutes treated with 200 micromol/L AOPP-BSA, the levels of phosphorylated ERK of ECV304 cells increased significantly (P < 0.01). When the ERK pathway was blocked by U0126, the promoting effects of AOPP-BSA on expressions of SDF-la protein in ECV304 cells were significantly inhibited in dose-dependent manner (P < 0.05).
  
  
  CONCLUSIONS
  AOPP induced the expression of SDF-la of ECV304 cells, ERK signal pathway is an important pathway that mediated the effects.
authors.affiliation: Department of Endocrinology, Dalian Medical University, Dalian 116011.
authors.forename: Chun-Hong
authors.initials: CH
authors.lastname: Shi
authors.forename: Yi-Nong
authors.initials: YN
authors.lastname: Jiang
authors.forename: Lu-Juan
authors.initials: LJ
authors.lastname: Shan
authors.forename: Yan
authors.initials: Y
authors.lastname: Lu
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhang
authors.forename: Yan-Guo
authors.initials: YG
authors.lastname: Gao
mesh_terms: D062107:Advanced Oxidation Protein Products / Q000494:pharmacology*; D002460:Cell Line; D054377:Chemokine CXCL12 / Q000378:metabolism*; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism*; D006801:Humans; D020935:MAP Kinase Signaling System; D018384:Oxidative Stress; D010766:Phosphorylation; D012333:RNA, Messenger / Q000235:genetics
chemical_list: D062107:Advanced Oxidation Protein Products; C515255:CXCL12 protein, human; D054377:Chemokine CXCL12; D012333:RNA, Messenger; D048049:Extracellular Signal-Regulated MAP Kinases