title: Regulated expression of PTPRJ/CD148 and an antisense long noncoding RNA in macrophages by proinflammatory stimuli.
abstract: PTPRJ/CD148 is a tyrosine phosphatase that has tumour suppressor-like activity. Quantitative PCR of various cells and tissues revealed that it is preferentially expressed in macrophage-enriched tissues. Within lymphoid tissues immunohistochemistry revealed that PTPRJ/CD148 co-localised with F4/80, indicating that macrophages most strongly express the protein. Macrophages express the highest basal level of ptprj, and this is elevated further by treatment with LPS and other Toll-like receptor ligands. In contrast, CSF-1 treatment reduced basal and stimulated Ptprj expression in human and mouse cells, and interferon also repressed Ptprj expression. We identified a 1006 nucleotide long noncoding RNA species, Ptprj-as1 that is transcribed antisense to Ptprj. Ptprj-as1 was highly expressed in macrophage-enriched tissue and was transiently induced by Toll-like receptor ligands with a similar time course to Ptprj. Finally, putative transcription factor binding sites in the promoter region of Ptprj were identified.
authors.affiliation: The University of Queensland, Institute for Molecular Bioscience, Brisbane, Australia.
authors.forename: Richa K
authors.initials: RK
authors.lastname: Dave
authors.forename: Marcel E
authors.initials: ME
authors.lastname: Dinger
authors.forename: Megan
authors.initials: M
authors.lastname: Andrew
authors.forename: Marjan
authors.initials: M
authors.lastname: Askarian-Amiri
authors.forename: David A
authors.initials: DA
authors.lastname: Hume
authors.forename: Stuart
authors.initials: S
authors.lastname: Kellie
mesh_terms: D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D057809:HEK293 Cells; D006801:Humans; D007249:Inflammation / Q000235:genetics* / Q000378:metabolism; D016173:Macrophage Colony-Stimulating Factor / Q000235:genetics / Q000378:metabolism; D008264:Macrophages / Q000378:metabolism*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D041681:NIH 3T3 Cells; D010586:Phagocytes / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D022661:RNA, Untranslated / Q000235:genetics* / Q000378:metabolism; D054631:Receptor-Like Protein Tyrosine Phosphatases, Class 3 / Q000235:genetics* / Q000378:metabolism*; D051193:Toll-Like Receptors / Q000235:genetics / Q000378:metabolism
chemical_list: D012333:RNA, Messenger; D022661:RNA, Untranslated; D051193:Toll-Like Receptors; D016173:Macrophage Colony-Stimulating Factor; D054631:Receptor-Like Protein Tyrosine Phosphatases, Class 3