title: Radiation affects the responsiveness of bone marrow to G-CSF.
abstract: In this study, we investigated the response of irradiated bone marrow cells to granulocyte colony-stimulating factor (G-CSF). Freshly harvested bone marrow cells were treated with either saline (vehicle control) or 20 ng/ml of G-CSF. Thereafter, cells were separated into nonirradiated (no-IR) and irradiated (IR, 0.5 Gy) groups. IR cells exhibited a higher proliferation rate in response to G-CSF, as compared to the no-IR cells. Reduced levels of reactive oxygen species indicated that G-CSF-treated IR cells produced fewer free radicals, as compared to the no-IR cells. The G-CSF-treated IR cells also had a lower apoptotic rate than their no-IR counterparts. Furthermore, G-CSF-treated IR cells exhibited less alteration of mitochondrial membrane potential, as compared to the no-IR cells. Finally, the mitochondrial number increased in the G-CSF-treated IR cells. The radiation-induced increase in plasma IL-6 in vivo could be enhanced by the administration of G-CSF. The data suggest that radiation potentiates the response of bone marrow cells to G-CSF treatment.
authors.affiliation: Department of Radiation Oncology, UF Shands Cancer Center, University of Florida, Gainesville, FL, 32610, USA.
authors.forename: Zhenhuan
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Department of Radiation Oncology, UF Shands Cancer Center, University of Florida, Gainesville, FL, 32610, USA.
authors.forename: Mei
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Department of Radiation Oncology, First Affiliated Hospital of Fujian Medical University, Fuzhou, Fujian, 35001, China.
authors.forename: Wenlong
authors.initials: W
authors.lastname: Lv
authors.affiliation: Department of Radiation Oncology, UF Shands Cancer Center, University of Florida, Gainesville, FL, 32610, USA.
authors.forename: Luqiang
authors.initials: L
authors.lastname: Huang
authors.affiliation: Department of Radiation Oncology, UF Shands Cancer Center, University of Florida, Gainesville, FL, 32610, USA.
authors.forename: Liangjie
authors.initials: L
authors.lastname: Yin
authors.affiliation: Department of Radiation Oncology, UF Shands Cancer Center, University of Florida, Gainesville, FL, 32610, USA.
authors.forename: Shanmin
authors.initials: S
authors.lastname: Yang
authors.affiliation: Department of Radiation Oncology, First Affiliated Hospital of Fujian Medical University, Fuzhou, Fujian, 35001, China.
authors.forename: Jinsheng
authors.initials: J
authors.lastname: Hong
authors.affiliation: Department of Radiation Oncology, First Affiliated Hospital of Fujian Medical University, Fuzhou, Fujian, 35001, China.
authors.forename: Deping
authors.initials: D
authors.lastname: Han
authors.affiliation: Department of Radiation Oncology, First Affiliated Hospital of Fujian Medical University, Fuzhou, Fujian, 35001, China.
authors.forename: Chun
authors.initials: C
authors.lastname: Chen
authors.affiliation: Department of Radiation Oncology, UF Shands Cancer Center, University of Florida, Gainesville, FL, 32610, USA.
authors.forename: Amy
authors.initials: A
authors.lastname: Zhang
authors.affiliation: Department of Radiation Oncology, UF Shands Cancer Center, University of Florida, Gainesville, FL, 32610, USA.
authors.forename: Sadasivan
authors.initials: S
authors.lastname: Vidyasagar
authors.affiliation: Department of Radiation Oncology, UF Shands Cancer Center, University of Florida, Gainesville, FL, 32610, USA.
authors.forename: Paul
authors.initials: P
authors.lastname: Okunieff
authors.affiliation: Department of Radiation Oncology, UF Shands Cancer Center, University of Florida, Gainesville, FL, 32610, USA. lurongzhang@ufl.edu.
authors.forename: Lurong
authors.initials: L
authors.lastname: Zhang
mesh_terms: D000818:Animals; D001853:Bone Marrow / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects*; D001854:Bone Marrow Cells / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D005609:Free Radicals / Q000378:metabolism; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology*; D015850:Interleukin-6 / Q000097:blood; D008297:Male; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects / Q000528:radiation effects; D051379:Mice; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D005609:Free Radicals; D015850:Interleukin-6; D017382:Reactive Oxygen Species; D016179:Granulocyte Colony-Stimulating Factor