title: Peptidomic Identification of Serum Peptides Diagnosing Preeclampsia.
abstract: We sought to identify serological markers capable of diagnosing preeclampsia (PE). We performed serum peptide analysis (liquid chromatography mass spectrometry) of 62 unique samples from 31 PE patients and 31 healthy pregnant controls, with two-thirds used as a training set and the other third as a testing set. Differential serum peptide profiling identified 52 significant serum peptides, and a 19-peptide panel collectively discriminating PE in training sets (n = 21 PE, n = 21 control; specificity = 85.7% and sensitivity = 100%) and testing sets (n = 10 PE, n = 10 control; specificity = 80% and sensitivity = 100%). The panel peptides were derived from 6 different protein precursors: 13 from fibrinogen alpha (FGA), 1 from alpha-1-antitrypsin (A1AT), 1 from apolipoprotein L1 (APO-L1), 1 from inter-alpha-trypsin inhibitor heavy chain H4 (ITIH4), 2 from kininogen-1 (KNG1), and 1 from thymosin beta-4 (TMSB4). We concluded that serum peptides can accurately discriminate active PE. Measurement of a 19-peptide panel could be performed quickly and in a quantitative mass spectrometric platform available in clinical laboratories. This serum peptide panel quantification could provide clinical utility in predicting PE or differential diagnosis of PE from confounding chronic hypertension.
authors.affiliation: Department of Surgery, Stanford University, Stanford, California, United States of America.
authors.forename: Qiaojun
authors.initials: Q
authors.lastname: Wen
authors.forename: Linda Y
authors.initials: LY
authors.lastname: Liu
authors.forename: Ting
authors.initials: T
authors.lastname: Yang
authors.forename: Cantas
authors.initials: C
authors.lastname: Alev
authors.forename: Shuaibin
authors.initials: S
authors.lastname: Wu
authors.forename: David K
authors.initials: DK
authors.lastname: Stevenson
authors.forename: Guojun
authors.initials: G
authors.lastname: Sheng
authors.forename: Atul J
authors.initials: AJ
authors.lastname: Butte
authors.forename: Xuefeng B
authors.initials: XB
authors.lastname: Ling
mesh_terms: D000328:Adult; D000075944:Apolipoprotein L1; D001053:Apolipoproteins / Q000737:chemistry; D015415:Biomarkers / Q000097:blood; D001798:Blood Proteins / Q000737:chemistry; D002853:Chromatography, Liquid / Q000379:methods*; D005260:Female; D006023:Glycoproteins / Q000737:chemistry; D006801:Humans; D007704:Kininogens / Q000737:chemistry; D008075:Lipoproteins, HDL / Q000737:chemistry; D010446:Peptide Fragments / Q000097:blood*; D011225:Pre-Eclampsia / Q000097:blood / Q000175:diagnosis*; D011247:Pregnancy; D053491:Proteinase Inhibitory Proteins, Secretory / Q000737:chemistry; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*; D013947:Thymosin / Q000737:chemistry; D055815:Young Adult; D000515:alpha 1-Antitrypsin / Q000737:chemistry; D000099201:Thymosin beta(4)
chemical_list: D000075944:Apolipoprotein L1; D001053:Apolipoproteins; D015415:Biomarkers; D001798:Blood Proteins; D006023:Glycoproteins; D007704:Kininogens; D008075:Lipoproteins, HDL; D010446:Peptide Fragments; D053491:Proteinase Inhibitory Proteins, Secretory; D013947:Thymosin; D000515:alpha 1-Antitrypsin; D000099201:Thymosin beta(4); C108666:APOL1 protein, human; C091236:ITIH4 protein, human; C000591034:KNG1 protein, human; C043392:SERPINA1 protein, human