title: Investigation of macrophage polarization using bone marrow derived macrophages.
abstract: The article describes a readily easy adaptive in vitro model to investigate macrophage polarization. In the presence of GM-CSF/M-CSF, hematopoietic stem/progenitor cells from the bone marrow are directed into monocytic differentiation, followed by M1 or M2 stimulation. The activation status can be tracked by changes in cell surface antigens, gene expression and cell signaling pathways.
authors.affiliation: Department of Animal Science, Texas A&M University, TX, USA.
authors.forename: Wei
authors.initials: W
authors.lastname: Ying
authors.forename: Patali S
authors.initials: PS
authors.lastname: Cheruku
authors.forename: Fuller W
authors.initials: FW
authors.lastname: Bazer
authors.forename: Stephen H
authors.initials: SH
authors.lastname: Safe
authors.forename: Beiyan
authors.initials: B
authors.lastname: Zhou
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D016764:Cell Polarity / Q000187:drug effects / Q000502:physiology; D003584:Cytological Techniques / Q000379:methods*; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000187:drug effects; D008262:Macrophage Activation / Q000187:drug effects / Q000502:physiology; D016173:Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D008264:Macrophages / Q000166:cytology* / Q000187:drug effects; D051379:Mice
chemical_list: D016173:Macrophage Colony-Stimulating Factor; D016178:Granulocyte-Macrophage Colony-Stimulating Factor