title: Synthetic RNA Silencing of Actinorhodin Biosynthesis in Streptomyces coelicolor A3(2).
abstract: We demonstrate the first application of synthetic RNA gene silencers in Streptomyces coelicolor A3(2). Peptide nucleic acid and expressed antisense RNA silencers successfully inhibited actinorhodin production. Synthetic RNA silencing was target-specific and is a new tool for gene regulation and metabolic engineering studies in Streptomyces.
authors.affiliation: School of Biology, Newcastle University, Newcastle upon Tyne, United Kingdom.
authors.forename: Gabriel C
authors.initials: GC
authors.lastname: Uguru
authors.forename: Madhav
authors.initials: M
authors.lastname: Mondhe
authors.forename: Shan
authors.initials: S
authors.lastname: Goh
authors.forename: Andrew
authors.initials: A
authors.lastname: Hesketh
authors.forename: Mervyn J
authors.initials: MJ
authors.lastname: Bibb
authors.forename: Liam
authors.initials: L
authors.lastname: Good
authors.forename: James E M
authors.initials: JE
authors.lastname: Stach
mesh_terms: D000880:Anthraquinones / Q000037:antagonists & inhibitors / Q000378:metabolism; D000900:Anti-Bacterial Agents / Q000096:biosynthesis*; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D020135:Peptide Nucleic Acids / Q000235:genetics*; D034622:RNA Interference; D016372:RNA, Antisense / Q000235:genetics*; D048372:Streptomyces coelicolor / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D000095622:Benzoisochromanequinones
chemical_list: D000880:Anthraquinones; D000900:Anti-Bacterial Agents; D020135:Peptide Nucleic Acids; D016372:RNA, Antisense; C013390:actinorhodin; D000095622:Benzoisochromanequinones