title: Molecular fingerprints to identify Candida species.
abstract: A wide range of molecular techniques have been developed for genotyping Candida species. Among them, multilocus sequence typing (MLST) and microsatellite length polymorphisms (MLP) analysis have recently emerged. MLST relies on DNA sequences of internal regions of various independent housekeeping genes, while MLP identifies microsatellite instability. Both methods generate unambiguous and highly reproducible data. Here, we review the results achieved by using these two techniques and also provide a brief overview of a new method based on high-resolution DNA melting (HRM). This method identifies sequence differences by subtle deviations in sample melting profiles in the presence of saturating fluorescent DNA binding dyes.
authors.affiliation: Departamento de Química Biológica, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario (UNR), Suipacha 531, 2000 Rosario, Argentina. spampinato@cefobi-conicet.gov.ar
authors.forename: Claudia
authors.initials: C
authors.lastname: Spampinato
authors.forename: Darío
authors.initials: D
authors.lastname: Leonardi
mesh_terms: D002175:Candida / Q000235:genetics* / Q000302:isolation & purification*; D016172:DNA Fingerprinting / Q000379:methods*; D018895:Microsatellite Repeats / Q000235:genetics; D058885:Multilocus Sequence Typing; D009691:Nucleic Acid Denaturation; D013045:Species Specificity