title: Chemical control of yeast cell division by cross-linked shells of catechol-grafted polyelectrolyte multilayers.
abstract: The chemical control of cell division has attracted much attention in the areas of single cell-based biology and high-throughput screening platforms. A mussel-inspired cytocompatible encapsulation method for achieving a "cell-division control" with cross-linked layer-by-layer (LbL) shells is developed. Catechol-grafted polyethyleneimine and hyaluronic acid are chosen as polyelectrolytes for the LbL process, and the cross-linking of polyelectrolytes is performed at pH 8.5. Cell division is controlled by the number of the LbL nanolayers and cross-linking reaction. We also suggest a new measuring unit, t-2.0 OD 600, for quantifying "cell-division timing" based on microbial growth kinetics.
authors.affiliation: Center for Cell-Encapsulation Research and Molecular-Level Interface Research Center, Department of Chemistry, KAIST, Daejeon, 305-701, Korea.
authors.forename: Juno
authors.initials: J
authors.lastname: Lee
authors.forename: Sung Ho
authors.initials: SH
authors.lastname: Yang
authors.forename: Seok-Pyo
authors.initials: SP
authors.lastname: Hong
authors.forename: Daewha
authors.initials: D
authors.lastname: Hong
authors.forename: Haeshin
authors.initials: H
authors.lastname: Lee
authors.forename: Hee-Yoon
authors.initials: HY
authors.lastname: Lee
authors.forename: Yang-Gyun
authors.initials: YG
authors.lastname: Kim
authors.forename: Insung S
authors.initials: IS
authors.lastname: Choi
mesh_terms: D002396:Catechols / Q000737:chemistry*; D002455:Cell Division; D006820:Hyaluronic Acid / Q000737:chemistry*; D011094:Polyethyleneimine / Q000737:chemistry*; D012441:Saccharomyces cerevisiae / Q000378:metabolism* / Q000648:ultrastructure
chemical_list: D002396:Catechols; D011094:Polyethyleneimine; D006820:Hyaluronic Acid; C034221:catechol
keywords: biomimetic synthesis; cell division; encapsulation; self-assembly; yeast