title: Two immunosuppressive compounds from the mushroom Rubinoboletus ballouii using human peripheral blood mononuclear cells by bioactivity-guided fractionation.
abstract: Rubinoboletus ballouii is an edible mushroom wildly grown in Yunnan province, China. Up till now, little was known about the chemical and biological properties of this mushroom. The aim of this study was to investigate the immunomodulatory effects of the ethanolic extract of Rubinoboletus ballouii and its fractions on human peripheral blood mononuclear cells (PBMCs) using bioactivity-guided fractionation. The crude extract of the fruiting bodies of RB was fractionated by high-speed counter current chromatography (HSCCC). Twelve fractions were obtained and the third fraction (Fraction C) exerted the most potent anti-inflammatory activities in mitogen-activated PBMCs. Further fractionation of fraction C led to the isolation of two single compounds which were elucidated as 1-ribofuranosyl-s-triazin-2(1H)-one and pistillarin, respectively. The results showed that both 1-ribofuranosyl-s-triazin-2(1H)-one and pistillarin exhibited significant immunosuppressive effects on phytohemagglutinin (PHA)-stimulated human PBMCs by inhibiting [methyl-(3)H]-thymidine uptake and inflammatory cytokines productions such as tumor necrosis factor (TNF)-α, interleukin (IL)-10, interferon (IFN)-γ and IL-1β. Besides, 1-ribofuranosyl-s-triazin-2(1H)-one was firstly found in natural resources, and pistillarin was also isolated from the family Boletaceae for the first time. They exhibited great potential in developing as anti-inflammatory reagents.
authors.affiliation: Institute of Chinese Medicine, The Chinese University of Hong Kong, Shatin, N. T., Hong Kong SAR, China; State Key Laboratory of Phytochemistry and Plant Resources in West China (CUHK), The Chinese University of Hong Kong, Shatin, N. T., Hong Kong SAR, China.
authors.forename: Long-Fei
authors.initials: LF
authors.lastname: Li
authors.forename: Ben Chung-Lap
authors.initials: BC
authors.lastname: Chan
authors.forename: Grace Gar-Lee
authors.initials: GG
authors.lastname: Yue
authors.forename: Clara Bik-San
authors.initials: CB
authors.lastname: Lau
authors.forename: Quan-Bin
authors.initials: QB
authors.lastname: Han
authors.forename: Ping-Chung
authors.initials: PC
authors.lastname: Leung
authors.forename: Ji-Kai
authors.initials: JK
authors.lastname: Liu
authors.forename: Kwok-Pui
authors.initials: KP
authors.lastname: Fung
mesh_terms: D000363:Agaricales / Q000737:chemistry*; D001681:Biological Assay; D002396:Catechols / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D002453:Cell Cycle / Q000187:drug effects; D002457:Cell Extracts / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D005591:Chemical Fractionation; D016207:Cytokines / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D007166:Immunosuppressive Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000378:metabolism; D015394:Molecular Structure; D012263:Ribonucleosides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D014227:Triazines / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology
chemical_list: C000590954:1-ribofuranosyl-s-triazin-2(1H)-one; D002396:Catechols; D002457:Cell Extracts; D016207:Cytokines; D007166:Immunosuppressive Agents; D012263:Ribonucleosides; D014227:Triazines; C000590955:pistillarin
keywords: 1-ribofuranosyl-s-triazin-2(1H)-one; Pistillarin; Rubinoboletus ballouii; bioassay-guided fractionation; peripheral blood mononuclear cells (PBMCs)