title: Discovery of 'molecular switches' within a GIRK activator scaffold that afford selective GIRK inhibitors.
abstract: This letter describes a multi-dimensional SAR campaign based on a potent, efficacious and selective GIRK1/2 activator (~10-fold versus GIRK1/4 and inactive on nonGIRK 1-containing GIRKs, GIRK 2 or GIRK2/3). Further chemical optimization through an iterative parallel synthesis effort identified multiple 'molecular switches' that modulated the mode of pharmacology from activator to inhibitor, as well as engendering varying selectivity profiles for GIRK1/2 and GIRK1/4. Importantly, these compounds were all inactive on nonGIRK1 containing GIRK channels. However, SAR was challenging as subtle structural modifications had large effects on both mode of pharmacology and GIRK1/2 and GIRK1/4 channel selectivity.
authors.affiliation: College of Science, Northwest Agriculture & Forestry University, Yangling, Shaanxi 712100, China.
authors.forename: Wandong
authors.initials: W
authors.lastname: Wen
authors.forename: Wenjun
authors.initials: W
authors.lastname: Wu
authors.forename: Ian M
authors.initials: IM
authors.lastname: Romaine
authors.forename: Kristian
authors.initials: K
authors.lastname: Kaufmann
authors.forename: Yu
authors.initials: Y
authors.lastname: Du
authors.forename: Gary A
authors.initials: GA
authors.lastname: Sulikowski
authors.forename: C David
authors.initials: CD
authors.lastname: Weaver
authors.forename: Craig W
authors.initials: CW
authors.lastname: Lindsley
mesh_terms: D001711:Biotransformation; D055808:Drug Discovery; D051676:G Protein-Coupled Inwardly-Rectifying Potassium Channels / Q000037:antagonists & inhibitors* / Q000737:chemistry; D008958:Models, Molecular; D015394:Molecular Structure; D004364:Pharmaceutical Preparations / Q000138:chemical synthesis* / Q000737:chemistry; D011485:Protein Binding; D013329:Structure-Activity Relationship
chemical_list: D051676:G Protein-Coupled Inwardly-Rectifying Potassium Channels; D004364:Pharmaceutical Preparations
keywords: Activators; GIRK; Inhibitors; K(ir)3.x; Thallium flux