title: Intracerebroventricular injection of stresscopin-related peptide enhances cardiovascular function in conscious rats.
abstract: Stresscopin-related peptide (SRP), which is a member of the corticotropin-releasing factor (CRF) family, is a high-affinity ligand for the type 2 corticotropin-releasing factor receptor (CRF-R2) and is involved in stress-coping responses. Central treatment with SRP suppresses food intake, delays gastric emptying and decreases heat-induced edema, but the effects of central administration of SRP on the cardiovascular system are unclear. Here we examined the effects of intracerebroventricular (i.c.v.) administration of SRP on cardiovascular function, and compared the cardiovascular effects of SRP and stresscopin (SCP). Our results showed that i.c.v. administration of SRP (0.5nmol) increased mean arterial blood pressure (MABP) and heart rate (HR), but failed to increase plasma norepinephrine and epinephrine levels. Compared with an equivalent dose of SCP, the area under the curve (AUC) values for the changes in MABP and HR were significantly smaller with SRP, indicating that the cardiovascular effects of SRP were weaker than those mediated by SCP. Pre-treatment with a selective CRF-R2 antagonist, antisauvagine-30 (4nmol, i.c.v.) abolished the SRP and SCP induced changes in MABP and HR. These results indicate that central administration of SRP induces a weaker enhancement of cardiovascular function through CRF-R2 than that induced by SCP and that these effects are mediated without increasing plasma norepinephrine and epinephrine levels.
authors.affiliation: Cellular Function Research Center, Yanbian University, 977 GongYuan Road, Yanji City, Jilin Province 133002, China; Department of Osteology, Affiliated Hospital of Yanbian University, Yanji City, Jilin Province, China.
authors.forename: Ri
authors.initials: R
authors.lastname: Jin
authors.forename: Mei-Zi
authors.initials: MZ
authors.lastname: Li
authors.forename: Yan-Hua
authors.initials: YH
authors.lastname: Bing
authors.forename: Ri-Long
authors.initials: RL
authors.lastname: Piao
authors.forename: Ying-Jun
authors.initials: YJ
authors.lastname: Li
authors.forename: Qing-Hua
authors.initials: QH
authors.lastname: Jin
authors.forename: De-Lai
authors.initials: DL
authors.lastname: Qiu
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kannan
authors.forename: Chun-Ping
authors.initials: CP
authors.lastname: Chu
mesh_terms: D000818:Animals; D001794:Blood Pressure / Q000187:drug effects; D003346:Corticotropin-Releasing Hormone / Q000008:administration & dosage / Q000502:physiology*; D004837:Epinephrine / Q000097:blood; D006339:Heart Rate / Q000187:drug effects; D006801:Humans; D007276:Injections, Intraventricular; D008297:Male; D009638:Norepinephrine / Q000097:blood; D010446:Peptide Fragments / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D018019:Receptors, Corticotropin-Releasing Hormone / Q000037:antagonists & inhibitors / Q000378:metabolism; D054832:Urocortins / Q000008:administration & dosage / Q000502:physiology*
chemical_list: D010446:Peptide Fragments; D018019:Receptors, Corticotropin-Releasing Hormone; C426705:UCN2 protein, human; C426704:UCN3 protein, human; D054832:Urocortins; C116607:antisauvagine 30; D003346:Corticotropin-Releasing Hormone; D009638:Norepinephrine; D004837:Epinephrine
keywords: Epinephrine; Heart rate; Mean arterial blood pressure; Neuropeptide; Norepinephrine