title: Chemical composition and antioxidant activity of a Lebanese plant Euphorbia macroclada schyzoceras.
abstract: OBJECTIVE
  To determine the chemical composition, total phenolic and total flavonoid contents of the crude extracts from leaves and stems of a Lebanese plant Euphorbia macroclada schyzoceras (E. macroclada), and to evaluate their antioxidant potential using DPPH, H2O2, and chelating of ferrous ions tests.
  
  
  METHODS
  Quantification of the total phenolic and total flavonoid contents of the crude extracts from leaves and stems and the antioxidant activities were evaluated using spectrophotometric analyses. The chemical composition has been estimated using different techniques such as IR, LC/MS and NMR.
  
  
  RESULTS
  Ethanolic extract from leaves of E. macroclada was better than aqueous extract and showed higher content in total phenolic and total flavonoid than found in the stems. On the other hand, using DPPH and H2O2 tests, this extract from leaves showed higher antioxidant capacity than aqueous extract. However, using the chelating of ferrous ions test, the antioxidant activity of the aqueous extract of both stems and leaves was stronger than that of ethanolic once. The chemical composition of the whole plant showed the presence of some aromatic compounds and fatty acids.
  
  
  CONCLUSIONS
  Both ethanolic and water extracts from both parts of this plant are effective and have good antioxidant power. So, this plant can be used in the prevention of a number of diseases related to oxidative stress.
authors.affiliation: Doctoral School of Science and Technology, Research Platform for Environmental Science, Lebanese University, Lebanon.
authors.forename: Hussein
authors.initials: H
authors.lastname: Farhan
authors.forename: Hassan
authors.initials: H
authors.lastname: Rammal
authors.forename: Akram
authors.initials: A
authors.lastname: Hijazi
authors.forename: Ahmad
authors.initials: A
authors.lastname: Daher
authors.forename: Mohamad
authors.initials: M
authors.lastname: Reda
authors.forename: Hussein
authors.initials: H
authors.lastname: Annan
authors.forename: Ali
authors.initials: A
authors.lastname: Chokr
authors.forename: Ali
authors.initials: A
authors.lastname: Bassal
authors.forename: Bassam
authors.initials: B
authors.lastname: Badran
mesh_terms: D000975:Antioxidants / Q000737:chemistry*; D028481:Euphorbia / Q000737:chemistry*; D005419:Flavonoids / Q000032:analysis / Q000737:chemistry*; D007861:Lebanon; D010636:Phenols / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry*; D018547:Plant Stems / Q000737:chemistry*; D013053:Spectrophotometry
chemical_list: D000975:Antioxidants; D005419:Flavonoids; D010636:Phenols; D010936:Plant Extracts
keywords: Antioxidant potential; Chemical composition; Euphorbia macroclada schyzoceras; Total flavonoid; Total phenolic