title: [The effect of LPS on airway inflammation, airway remodeling and TLR4 expression in asthmatic rat].
abstract: OBJECTIVE
  To investigate the effects of lipopolysaccharide (LPS) on airway inflammation, airway remodeling and the expression of Toll-like receptor 4 (TLR4) mRNA in asthmatic rats.
  
  
  METHODS
  Twenty-four SPF level SD rats were randomly divided into four groups (n = 6): control group, low dose of LPS group, high dose of LPS group and asthma group. Using ovalbumin (OVA) to sensitize and challenge to establish asthmatic rat model. Observed pathological changes of lung tissue by HE staining, inflammatory cell infiltration was observed by airway wall eosinophils (EOS) counts; airway resistance was determined; image analysis software was used to determine the thickness of airway wall, detected airway smooth muscle TLR4 expression levels by RT-PCR.
  
  
  RESULTS
  The rat airway resistance and the EOS number of airway wall and the thickness of airway wall in asthma group, low dose of LPS group and high dose of LPS group were significantly higher than those in control group (P < 0.01). The above-mentioned parameters of high dose of LPS group showed significantly lower than those in asthma group and low dose of LPS group (P < 0.05). The expression of rat airway smooth muscle TLR4 mRNA in low dose of LPS group and high dose of LPS group were significantly higher than those in asthma group (P < 0.01). And the expression of rat airway smooth muscle TLR4 mRNA in high dose of LPS group was significantly higher than that in low dose of LPS group (P < 0.05).
  
  
  CONCLUSIONS
  TLR4 plays an important role in asthmatic airway inflammation and airway remodeling, LPS may play double-sided regulation in asthmatic airway inflammation and airway remodeling by activated TLR4.
authors.affiliation: Department of Respiratory Medicine, Hospital Affiliated Guilin Medical College, Guilin 541001, China. mobiwen2002@sohu.com
authors.forename: Bi-Wen
authors.initials: BW
authors.lastname: Mo
authors.forename: Zhen-Zhen
authors.initials: ZZ
authors.lastname: Zhang
authors.forename: Jiang-Hong
authors.initials: JH
authors.lastname: Wei
authors.forename: Jian-Wei
authors.initials: JW
authors.lastname: Huang
authors.forename: Bi-Fan
authors.initials: BF
authors.lastname: Mo
authors.forename: Chang-Ming
authors.initials: CM
authors.lastname: Wang
authors.forename: Jin-Rong
authors.initials: JR
authors.lastname: Zeng
authors.forename: Qing
authors.initials: Q
authors.lastname: Xu
authors.forename: Yun
authors.initials: Y
authors.lastname: Lin
mesh_terms: D056151:Airway Remodeling / Q000187:drug effects*; D000818:Animals; D001249:Asthma / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology; D007249:Inflammation / Q000378:metabolism; D008070:Lipopolysaccharides / Q000009:adverse effects / Q000494:pharmacology*; D008168:Lung / Q000378:metabolism / Q000503:physiopathology*; D008297:Male; D009130:Muscle, Smooth / Q000187:drug effects / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D051197:Toll-Like Receptor 4 / Q000378:metabolism*
chemical_list: D008070:Lipopolysaccharides; C495347:Tlr4 protein, rat; D051197:Toll-Like Receptor 4