title: Deglycosylation is a key step in biotransformation and lifespan effects of quercetin-3-O-glucoside in Caenorhabditis elegans.
abstract: Due to their purported healthful activities, quercetin and other flavonoids are being increasingly proposed as nutraceuticals. Quercetin occurs in food as glycosides; however, most assays on its activity have been performed with the aglycone, despite glycosylation deeply affects compound bioavailability. In this work, the uptake and lifespan effects of quercetin-3-O-glucoside (Q3Glc) and quercetin have been assessed in Caenorhabditis elegans. Q3Glc was taken up by this nematode in a concentration-dependent manner and rapidly deglycosylated to quercetin, which was accumulated in the worm and partially biotransformed to conjugated metabolites. Significant mean lifespan extension up to 23% compared to controls was observed in wild type worms cultivated in the presence of low concentrations of Q3Glc (10 μM and 25 μM), whereas exposure to greater concentrations of Q3Glc (50-200 μM) caused a reduction in mean and maximum lifespan compared with the control. By contrast, treatment of klo-1 and klo-2 mutant worms lacking β-glucosidase activity with 200 μM of Q3Glc led to extended mean lifespan (up to 39%), similar to quercetin aglycone at the same concentration levels. In those mutants, Q3Glc was accumulated without important deglycosylation to quercetin was produced. Taken together, these findings indicated that Q3Glc was taken up by the nematode in greater extent than quercetin, and that deglycosylation and subsequent aglycone accumulation in the worm appeared as key points to explain the observed lifespan effects. The obtained results also suggested that facilitated absorption should be more important for the uptake of quercetin derivatives than passive diffusion.
authors.affiliation: Grupo de Investigación en Polifenoles, Unidad de Nutrición y Bromatología, Facultad de Farmacia, Universidad de Salamanca, Campus Miguel de Unamuno, 37007 Salamanca, Spain.
authors.forename: Montserrat
authors.initials: M
authors.lastname: Dueñas
authors.forename: Felipe
authors.initials: F
authors.lastname: Surco-Laos
authors.forename: Susana
authors.initials: S
authors.lastname: González-Manzano
authors.forename: Ana M
authors.initials: AM
authors.lastname: González-Paramás
authors.forename: Eva
authors.initials: E
authors.lastname: Gómez-Orte
authors.forename: Juan
authors.initials: J
authors.lastname: Cabello
authors.forename: Celestino
authors.initials: C
authors.lastname: Santos-Buelga
mesh_terms: D000818:Animals; D001711:Biotransformation; D017173:Caenorhabditis elegans / Q000187:drug effects* / Q000235:genetics / Q000502:physiology*; D005419:Flavonoids / Q000378:metabolism / Q000493:pharmacokinetics* / Q000494:pharmacology*; D005960:Glucosides; D006031:Glycosylation; D008958:Models, Molecular; D009154:Mutation; D011794:Quercetin / Q000031:analogs & derivatives
chemical_list: D005419:Flavonoids; D005960:Glucosides; C532085:quercetin 3'-O-glucoside; D011794:Quercetin
keywords: Absorption; Caenorhabditis elegans; Deglycosylation; Lifespan; Mutant worms; Quercetin; klo-1; klo-2; β-Glucosidases