title: Membrane protein complex of APS reductase and Qmo is present in Desulfovibrio vulgaris and Desulfovibrio alaskensis.
abstract: Due to their adjacent location in the genomes of Desulfovibrio species and their potential for formation of an electron transfer pathway in sulfate-reducing prokaryotes, adenosyl phosphosulfate (APS) reductase (Apr) and quinone-interacting membrane-bound oxidoreductase (Qmo) have been thought to interact together during the reduction of APS. This interaction was recently verified in Desulfovibrio desulfuricans. Membrane proteins of Desulfovibrio vulgaris Hildenborough ΔqmoABCD JW9021, a deletion mutant, were compared to the parent strain using blue-native PAGE to determine whether Qmo formed a complex with Apr or other proteins. In the parent strain of D. vulgaris, a unique band was observed that contained all four Qmo subunits, and another band contained three subunits of Qmo, as well as subunits of AprA and AprB. Similar results were observed with bands excised from membrane preparations of Desulfovibrio alaskensis strain G20. These results are in support of the formation of a physical complex between the two proteins; a result that was further confirmed by the co-purification of QmoA/B and AprA/B from affinity-tagged D. vulgaris Hildenborough strains (AprA, QmoA and QmoB) regardless of which subunit had been tagged. This provides clear evidence for the presence of a Qmo-Apr complex that is at least partially stable in protein extracts of D. vulgaris and D. alaskensis.
authors.affiliation: Institute for Energy and the Environment, University of Oklahoma, Norman, OK 73019, USA.|Department of Botany and Microbiology, University of Oklahoma, Norman, OK 73019, USA.
authors.forename: Lee R
authors.initials: LR
authors.lastname: Krumholz
authors.affiliation: Department of Botany and Microbiology, University of Oklahoma, Norman, OK 73019, USA.
authors.forename: Luyao
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Chemical Engineering, University of Washington, Seattle, WA 98195, USA.|eScience Institute, University of Washington, Seattle, WA 98195, USA.
authors.forename: David A C
authors.initials: DAC
authors.lastname: Beck
authors.affiliation: Center for Microbial Proteomics, University of Washington, Seattle, WA 98195, USA.|Department of Microbiology, University of Washington, Seattle, WA 98195, USA.
authors.forename: Tiansong
authors.initials: T
authors.lastname: Wang
authors.affiliation: Center for Microbial Proteomics, University of Washington, Seattle, WA 98195, USA.|Department of Chemical Engineering, University of Washington, Seattle, WA 98195, USA.
authors.forename: Murray
authors.initials: M
authors.lastname: Hackett
authors.affiliation: Department of Biochemistry, University of Missouri, USA.
authors.forename: Brian
authors.initials: B
authors.lastname: Mooney
authors.affiliation: Department of Biochemistry, University of Missouri, USA.
authors.forename: Thomas R
authors.initials: TR
authors.lastname: Juba
authors.affiliation: Department of Botany and Microbiology, University of Oklahoma, Norman, OK 73019, USA.
authors.forename: Michael J
authors.initials: MJ
authors.lastname: McInerney
authors.affiliation: Department of Civil and Environmental Engineering, University of Washington, Seattle, WA 98195, USA.
authors.forename: Birte
authors.initials: B
authors.lastname: Meyer
authors.affiliation: Department of Biochemistry, University of Missouri, USA.
authors.forename: Judy D
authors.initials: JD
authors.lastname: Wall
authors.affiliation: Department of Civil and Environmental Engineering, University of Washington, Seattle, WA 98195, USA.
authors.forename: David A
authors.initials: DA
authors.lastname: Stahl
mesh_terms: D003901:Desulfovibrio / Q000737:chemistry* / Q000201:enzymology*; D017353:Gene Deletion; D008565:Membrane Proteins / Q000378:metabolism*; D016660:NAD(P)H Dehydrogenase (Quinone) / Q000378:metabolism*; D050862:Oxidoreductases Acting on Sulfur Group Donors / Q000378:metabolism*; D055503:Protein Multimerization
chemical_list: D008565:Membrane Proteins; D016660:NAD(P)H Dehydrogenase (Quinone); D050862:Oxidoreductases Acting on Sulfur Group Donors; C019618:adenylylsulfate reductase