title: Immobilized amyloid Aβ peptides support platelet adhesion and activation.
abstract: Accumulation of amyloidogenic Aβ peptides in the brain contributes to the onset of Alzheimer disease. Aβ peptide deposits are also present in blood vessel walls, mainly deriving from circulating platelets. However, their effect on platelet function is unclear. We demonstrate that immobilized Aβ peptides induce platelet adhesion and spreading through metalloproteinase-sensitive surface receptors. Aβ peptides also fasten platelet spreading on collagen, and support the time- and ADP-dependent activation of adherent platelets, leading to stimulation of several signalling proteins. Our results indicate a potential role for peripheral Aβ peptides in promoting platelet adhesion and activation in the initiation of thrombus formation.
authors.affiliation: Department of Biology and Biotechnology, Laboratory of Biochemistry, University of Pavia, Italy. ilaria.canobbio@unipv.it
authors.forename: Ilaria
authors.initials: I
authors.lastname: Canobbio
authors.forename: Silvia
authors.initials: S
authors.lastname: Catricalà
authors.forename: Laura G
authors.initials: LG
authors.lastname: Di Pasqua
authors.forename: Gianni
authors.initials: G
authors.lastname: Guidetti
authors.forename: Alessandra
authors.initials: A
authors.lastname: Consonni
authors.forename: Daria
authors.initials: D
authors.lastname: Manganaro
authors.forename: Mauro
authors.initials: M
authors.lastname: Torti
mesh_terms: D000244:Adenosine Diphosphate / Q000378:metabolism; D000544:Alzheimer Disease / Q000378:metabolism; D016229:Amyloid beta-Peptides / Q000378:metabolism*; D001808:Blood Vessels / Q000378:metabolism; D002448:Cell Adhesion; D002462:Cell Membrane / Q000378:metabolism; D002561:Cerebrovascular Disorders / Q000097:blood / Q000378:metabolism; D003094:Collagen / Q000378:metabolism; D006801:Humans; D055767:Immobilized Proteins / Q000378:metabolism; D045726:Metalloproteases / Q000378:metabolism; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism; D015539:Platelet Activation; D010973:Platelet Adhesiveness; D011493:Protein Kinase C / Q000378:metabolism; D013927:Thrombosis / Q000378:metabolism
chemical_list: D016229:Amyloid beta-Peptides; D055767:Immobilized Proteins; D000244:Adenosine Diphosphate; D003094:Collagen; D019869:Phosphatidylinositol 3-Kinases; D011493:Protein Kinase C; D045726:Metalloproteases
keywords: AD; APP; Adhesion; Alzheimer disease; Amyloid Aβ peptide; Cerebrovascular inflammation; Human platelet; PI3K; PKC; PLC; amyloid precursor protein; phosphatidylinositol 3 kinase; phospholipase C; protein kinase C