title: Preliminary assessment of potential toxicity of methylated soybean protein and methylated β-lactoglobulin in male Wistar rats.
abstract: Methylated soybean protein (MSP) and methylated β-lactoglobulin (MLG), previously confirmed for their antibacterial and antiviral activities, were tested for their potential toxicity in Wistar male Albino rats as one single dose (2500, 5000 and 10,000 mg/kg body wt) or as repeated daily dose (500 and 2500 mg/kg body wt/day) over 28 days to assess potential toxicity. Single acute administration of very high doses (2500, 5000 and 10,000 mg/kg body wt) of MSP and MLG did not produce any mortality. Changes in body weight, organ weight, hematological parameters, histo-pathological images of selected organs, serum albumin, globulin and albumin/globulin ratio, cholesterol, triglycerides and electrolytes were all within normal amounts in the rats fed with these two methylated proteins and not significantly different from controls. The levels of serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), creatinine and urea were slightly reduced by the administration of these two modified proteins indicating the absence of any adverse effect on hepatic or renal functions.
authors.affiliation: Biochemistry Department, Faculty of Agriculture, Zagazig University, Zagazig 44511, Egypt. mzsitohy@hotmail.com
authors.forename: Mahmoud
authors.initials: M
authors.lastname: Sitohy
authors.forename: Ali
authors.initials: A
authors.lastname: Osman
authors.forename: Ahmed
authors.initials: A
authors.lastname: Gharib
authors.forename: Jean-Marc
authors.initials: JM
authors.lastname: Chobert
authors.forename: Thomas
authors.initials: T
authors.lastname: Haertlé
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000009:adverse effects* / Q000378:metabolism; D000998:Antiviral Agents / Q000008:administration & dosage / Q000009:adverse effects* / Q000378:metabolism; D005520:Food Preservatives / Q000008:administration & dosage / Q000009:adverse effects* / Q000378:metabolism; D007782:Lactoglobulins / Q000008:administration & dosage / Q000009:adverse effects* / Q000378:metabolism; D008297:Male; D008745:Methylation; D019055:No-Observed-Adverse-Effect Level; D014674:Plant Proteins, Dietary / Q000008:administration & dosage / Q000009:adverse effects* / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D030262:Soybean Proteins / Q000008:administration & dosage / Q000009:adverse effects* / Q000378:metabolism; D023382:Toxicity Tests, Acute; D059551:Toxicity Tests, Subacute
chemical_list: D000900:Anti-Bacterial Agents; D000998:Antiviral Agents; D005520:Food Preservatives; D007782:Lactoglobulins; D014674:Plant Proteins, Dietary; D030262:Soybean Proteins
keywords: Hepatic function; Histopathology; Methylated proteins; Renal function; Serum proteins; Toxicity