title: HPLC fluorescence method for the determination of nizatidine in human plasma and its application to pharmacokinetic study.
abstract: A sensitive high-performance liquid chromatographic (HPLC) method was developed for the determination of nizatidine in human plasma. Nizatidine was derivatized by 4-fluoro-7-nitrobenzofurazan (NBD-F). Chromatographic separation was performed on a Inertsil C18 column (150 mm × 4.6 mm, 5 µm) using isocratic elution by a mobile phase consisting of methanol/water (55:45) at a flow rate of 1.2 mL/min. Amlodipine was used as the internal standard (IS). Fluorescence detector was used operated at 461 nm (excitation) and 517 nm (emission), respectively. The calibration curve was linear over the range of 50-2000 ng/mL. This method was successfully applied to a pharmacokinetic study after oral administration of a dose (150 mg) of nizatidine.
authors.affiliation: Istanbul University, Faculty of Pharmacy, Department of Analytical Chemistry, 34416, Istanbul, Turkey.
authors.forename: Mahmut B
authors.initials: MB
authors.lastname: Çakar
authors.forename: Sevgi T
authors.initials: ST
authors.lastname: Ulu
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D005453:Fluorescence; D006635:Histamine H2 Antagonists / Q000097:blood* / Q000493:pharmacokinetics; D006801:Humans; D016567:Nizatidine / Q000097:blood* / Q000493:pharmacokinetics
chemical_list: D006635:Histamine H2 Antagonists; D016567:Nizatidine
keywords: derivatization; fluorescence detector; nizatidine; pharmacokinetics