title: First evidence of autochthonous cases of Leishmania (Leishmania) infantum in horse (Equus caballus) in the Americas and mixed infection of Leishmania infantum and Leishmania (Viannia) braziliensis.
abstract: This study reports the first evidence of infection by Leishmania infantum in Equus caballus in Americas and the first mixed infection of L. infantum/Leishmania braziliensis on this mammalian species in the world. The diagnoses was based on presence of parasites in lesions and bone marrow aspirates, their identification by using specific primers for L. infantum and L. braziliensis complexes and also serological methods IFAT and ELISA. The analysis of the PCR products suggested mixed infection in three animals. Further studies involving equine leishmaniasis are carrying out in order to clarify the dynamic of Leishmania sp. in this mammalian specie and their role in the transmission of those parasites in urban endemic area of Belo Horizonte, Minas Gerais State, Brazil.
authors.affiliation: Departamento de Clínica e Cirurgia Veterinárias, Escola de Veterinária, Universidade Federal de Minas Gerais, Av. Antônio Carlos 6627, Pampulha, CEP: 30123-970, Belo Horizonte, MG, Brazil. Electronic address: bebelsoares@yahoo.com.br.
authors.forename: Isabel R
authors.initials: IR
authors.lastname: Soares
authors.forename: Soraia O
authors.initials: SO
authors.lastname: Silva
authors.forename: Filipe Moraghi
authors.initials: FM
authors.lastname: Moreira
authors.forename: Luan Gavião
authors.initials: LG
authors.lastname: Prado
authors.forename: Priscila
authors.initials: P
authors.lastname: Fantini
authors.forename: Renata de Pino Albuquerque
authors.initials: Rde P
authors.lastname: Maranhão
authors.forename: José Monteiro
authors.initials: JM
authors.lastname: da Silva Filho
authors.forename: Maria Norma
authors.initials: MN
authors.lastname: Melo
authors.forename: Maristela S
authors.initials: MS
authors.lastname: Palhares
mesh_terms: D000818:Animals; D001938:Brazil / Q000453:epidemiology; D060085:Coinfection; D005260:Female; D006734:Horse Diseases / Q000453:epidemiology / Q000469:parasitology* / Q000473:pathology; D006736:Horses; D007892:Leishmania braziliensis / Q000302:isolation & purification*; D018314:Leishmania infantum / Q000302:isolation & purification*; D007896:Leishmaniasis / Q000453:epidemiology / Q000469:parasitology / Q000473:pathology / Q000662:veterinary*; D008297:Male
keywords: Brazil; Equine; Leishmania braziliensis; Leishmania infantum; Mixed infection