title: Differences in the cytogenetic alteration profiles of diffuse large B-cell lymphoma among Chinese and American patients.
abstract: To study the similarities and differences of cytogenetic alterations in diffuse large B-cell lymphoma (DLBCL) between Asian and Caucasian patients, we compared the cytogenetic profiles of Chinese and American DLBCL cases by analyzing conventional karyotypes and select fluorescence in situ hybridization (FISH) findings. We used interphase FISH analyses to determine the incidence of the t(14;18) and BCL6 and MYC rearrangements. Immunohistochemical analysis was used to categorize the lymphomas into the germinal center B-cell-like (GCB) or non-GCB-DLBCL subtypes, according to the Hans algorithm. Our data suggested that Chinese patients had cytogenetic profiles for GCB-DLBCL that differed from those of their American counterparts; specifically, the Chinese GCB patients exhibited greater frequencies of BCL6 rearrangements and gains of 1q and 11q but lower incidence of the t(14;18). Non-GCB-DLBCL in both the Chinese and American patients was characterized by recurrent gains of 3/3q and 18/18q. The incidences of both BCL6 rearrangement and t(14;18) were similar in Chinese and American non-GCB-DLBCL cases.
authors.affiliation: Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, NE, USA.
authors.forename: Yan
authors.initials: Y
authors.lastname: Chen
authors.forename: Bhavana J
authors.initials: BJ
authors.lastname: Dave
authors.forename: Xiongzeng
authors.initials: X
authors.lastname: Zhu
authors.forename: Wing C
authors.initials: WC
authors.lastname: Chan
authors.forename: Javeed
authors.initials: J
authors.lastname: Iqbal
authors.forename: Warren G
authors.initials: WG
authors.lastname: Sanger
authors.forename: Kai
authors.initials: K
authors.lastname: Fu
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D044466:Asian People / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics*; D005260:Female; D015321:Gene Rearrangement; D016259:Genes, myc; D006801:Humans; D007150:Immunohistochemistry; D017404:In Situ Hybridization, Fluorescence; D015994:Incidence; D059785:Karyotype; D016403:Lymphoma, Large B-Cell, Diffuse / Q000208:ethnology / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D051560:Proto-Oncogene Proteins c-bcl-6; D044465:White People / Q000235:genetics
chemical_list: C084103:BCL6 protein, human; D004268:DNA-Binding Proteins; D051560:Proto-Oncogene Proteins c-bcl-6
keywords: Diffuse large B-cell lymphoma; Hans algorithm; activated B-cell-like DLBCL; germinal center B-cell–like DLBCL; immunohistochemistry