title: Generation of haploid embryonic stem cells from Macaca fascicularis monkey parthenotes.
abstract: Recent success in the derivation of haploid embryonic stem cells (haESCs) from mouse via parthenogenesis and androgenesis has enabled genetic screening in mammalian cells and generation of gene-modified animals. However, whether haESCs can be derived from primates remains unknown. Here, we report the derivation of haESCs from parthenogenetic blastocysts of Macaca fascicularis monkeys. These cells, termed as PG-haESCs, are pluripotent and can differentiate to cells of three embryonic germ layers in vitro or in vivo. Interestingly, the haploidy of one monkey PG-haESC line (MPH1) is more stable compared with that of the other one (MPH2), as shown by the existence of haploid cells for more than 140 days without fluorescence-activated cell sorting (FACS) enrichment of haploid cells. Importantly, transgenic monkey PG-haESC lines can be generated by lentivirus- and piggyBac transposon-mediated gene transfer. Moreover, genetic screening is feasible in monkey PG-haESCs. Our results demonstrate that PG-haESCs can be generated from monkeys, providing an ideal tool for genetic analyses in primates.
authors.affiliation: Group of Epigenetic Reprogramming, State Key Laboratory of Cell Biology, Shanghai Key Laboratory of Molecular Andrology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Yang
authors.forename: Zhen
authors.initials: Z
authors.lastname: Liu
authors.forename: Yu
authors.initials: Y
authors.lastname: Ma
authors.forename: Cuiqing
authors.initials: C
authors.lastname: Zhong
authors.forename: Qi
authors.initials: Q
authors.lastname: Yin
authors.forename: Chikai
authors.initials: C
authors.lastname: Zhou
authors.forename: Linyu
authors.initials: L
authors.lastname: Shi
authors.forename: Yijun
authors.initials: Y
authors.lastname: Cai
authors.forename: Hanzhi
authors.initials: H
authors.lastname: Zhao
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
authors.forename: Fan
authors.initials: F
authors.lastname: Tang
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.forename: Chenchen
authors.initials: C
authors.lastname: Zhang
authors.forename: Xin-Yuan
authors.initials: XY
authors.lastname: Liu
authors.forename: Dongmei
authors.initials: D
authors.lastname: Lai
authors.forename: Ying
authors.initials: Y
authors.lastname: Jin
authors.forename: Qiang
authors.initials: Q
authors.lastname: Sun
authors.forename: Jinsong
authors.initials: J
authors.lastname: Li
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000166:cytology; D002454:Cell Differentiation; D002478:Cells, Cultured; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000378:metabolism; D006238:Haploidy; D008252:Macaca fascicularis / Q000196:embryology* / Q000235:genetics; D010312:Parthenogenesis