title: Neuroprotective polyhydroxypregnane glycosides from Cynanchum otophyllum.
abstract: Five new polyhydroxypregnane glycosides, namely cynanotosides A-E (1-5), together with two known analogues, deacetylmetaplexigenin (6) and cynotophylloside H (7), were isolated from the roots of Cynanchum otophyllum. Their structures were established by spectroscopic methods and acid hydrolysis. The neuroprotective effects of compounds 1-7 against glutamate-, hydrogen peroxide-, and homocysteic acid (HCA)-induced cell death were tested by MTT assay in a hippocampal neuronal cell line HT22. Compounds 1, 2, and 7 exhibited protective activity against HCA-induced cell death in a dose-dependent manner ranging from 1 to 30μM, which may explain the Traditional Chinese Medicine (TCM) use of this plant for the treatment of epilepsy.
authors.affiliation: School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou, Guangdong 510006, China.
authors.forename: Zhi-Min
authors.initials: ZM
authors.lastname: Zhao
authors.forename: Zhang-Hua
authors.initials: ZH
authors.lastname: Sun
authors.forename: Mei-Hui
authors.initials: MH
authors.lastname: Chen
authors.forename: Qiong
authors.initials: Q
authors.lastname: Liao
authors.forename: Ming
authors.initials: M
authors.lastname: Tan
authors.forename: Xin-Wen
authors.initials: XW
authors.lastname: Zhang
authors.forename: Han-Dong
authors.initials: HD
authors.lastname: Zhu
authors.forename: Rong-Biao
authors.initials: RB
authors.lastname: Pi
authors.forename: Sheng
authors.initials: S
authors.lastname: Yin
mesh_terms: D000818:Animals; D016923:Cell Death; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D031105:Cynanchum / Q000737:chemistry*; D004353:Drug Evaluation, Preclinical; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D006027:Glycosides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D051379:Mice; D009474:Neurons / Q000187:drug effects / Q000502:physiology*; D018696:Neuroprotective Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects; D011278:Pregnanes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D004365:Drugs, Chinese Herbal; D006027:Glycosides; D018696:Neuroprotective Agents; D011278:Pregnanes
keywords: Cynanchum otophyllum; Neuroprotective effect; Polyhydroxypregnane glycosides; Structure elucidation