title: [Influence of BRAF interference on SW579 cell lines in thyroid cancer].
abstract: OBJECTIVE
  To determine the influence of v-raf murine sarcoma viral oncogene homolog B1 (BRAF) interference on SW579 cell lines in thyroid cancer.
  
  
  METHODS
  We designed 2 pairs of siRNA interference sequences, transfected them into SW579 cell line with liposome, and detected the interference with RT-PCR method. For successfully interfered cell lines, the changes in cell proliferation, cell cycle, and the expression of related proteins in mitogen-activated protein signal-regulated kinase /extracellular signal-regulated kinase (MEK/ ERK) signal pathway were detected.
  
  
  RESULTS
  After 2 pairs of siRNA transfection, the expressions of BRAF mRNA and protein of SW579 cell lines were significantly inhibited (P<0.01). The proliferation was inhibited, the cell cycle was changed, G1/S phase increased, and MEK/ERK signal pathway was inhibited.
  
  
  CONCLUSIONS
  Inhibition of growth and proliferation of SW579 cell lines by BRAF may be functioned by de-activating MEK/ERK signal pathway.
authors.affiliation: Department of General Surgery, Central South University, Changsha, China. yiwenjun@csco.org.cn
authors.forename: Wenjun
authors.initials: W
authors.lastname: Yi
authors.forename: Dewu
authors.initials: D
authors.lastname: Zhong
authors.forename: Qiongyan
authors.initials: Q
authors.lastname: Zou
mesh_terms: D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D006801:Humans; D020935:MAP Kinase Signaling System; D048493:Proto-Oncogene Proteins B-raf / Q000235:genetics / Q000378:metabolism*; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics*; D013964:Thyroid Neoplasms / Q000473:pathology*
chemical_list: D034741:RNA, Small Interfering; C482119:BRAF protein, human; D048493:Proto-Oncogene Proteins B-raf