title: Alterations in affective behavior during the time course of alcohol hangover.
abstract: Alcohol hangover is a temporary state described as the unpleasant next-day effects after binge-like drinking. Hangover begins when ethanol is absent in plasma and is characterized by physical and psychological symptoms. Affective behavior is impaired during the acute phase of alcohol intoxication; however, no reports indicate if similar effects are observed during withdrawal. The aim of this work was to study the time-extension and possible fluctuations in affective behavior during a hangover episode. Male Swiss mice were injected i.p. either with saline (control group) or with ethanol (3.8g/kg BW) (hangover group). Anxiety, fear-related behavior and despair phenotype were evaluated at a basal point (ZT0) and every 2h up to 20h after blood alcohol levels were close to zero (hangover onset). Also, anhedonia signs and pain perception disabilities were studied. Mice exhibited an increase in anxiety-like behavior during 4h and 14h after hangover onset when evaluated by the elevated-plus maze and open field test respectively (p<0.05). Fear-related behavior was detected in hangover animals by the increase of freezing and decrease of line crossings and rearing frequency during 16h after hangover onset (p<0.001). Depression signs were found in hangover mice during 14h (p<0.05). Hangover mice showed a significant decrease in pain perception when tested by tail immersion test at the beginning of hangover (p<0.05). Our findings demonstrate a time-extension between 14 and 16h for hangover affective impairments. This study shows the long lasting effects of hangover over the phase of ethanol intoxication.
authors.affiliation: Institute of Biochemistry and Molecular Medicine, School of Pharmacy and Biochemistry, University of Buenos Aires, Buenos Aires, Argentina. analiakaradayian@conicet.gov.ar
authors.forename: Analía G
authors.initials: AG
authors.lastname: Karadayian
authors.forename: María J
authors.initials: MJ
authors.lastname: Busso
authors.forename: Carlos
authors.initials: C
authors.lastname: Feleder
authors.forename: Rodolfo A
authors.initials: RA
authors.lastname: Cutrera
mesh_terms: D000339:Affect / Q000187:drug effects*; D000435:Alcoholic Intoxication / Q000523:psychology*; D059445:Anhedonia; D000818:Animals; D001007:Anxiety / Q000523:psychology; D002492:Central Nervous System Depressants / Q000009:adverse effects*; D003672:Defecation / Q000502:physiology; D000431:Ethanol / Q000009:adverse effects*; D005239:Fear / Q000523:psychology; D005518:Food Preferences; D019417:Hindlimb Suspension / Q000523:psychology; D006358:Hot Temperature; D007101:Immersion / Q000503:physiopathology; D008297:Male; D051379:Mice; D009043:Motor Activity / Q000502:physiology; D010147:Pain Measurement; D058748:Pain Perception / Q000187:drug effects; D010775:Photic Stimulation; D013395:Sucrose; D013550:Swimming / Q000523:psychology
chemical_list: D002492:Central Nervous System Depressants; D000431:Ethanol; D013395:Sucrose
keywords: %FEO; %TSO; AH; Alcohol hangover; Anhedonia; Anxiety-like behavior; BAC; Depression; EMP; Nociception; TE; ZT; alcohol hangover; blood alcohol concentration; elevated-plus maze; i.p.; intraperitoneally; proportion of entrance into open arms; proportion of time spent in open arms; total number of entries; zeitgeber time