title: Hyaluronic acid nanohydrogels as a useful tool for BSAO immobilization in the treatment of melanoma cancer cells.
abstract: An alternative anticancer therapy based on the use of bovine serum amine oxidase (BSAO), an enzyme that converts polyamines over-expressed in malignant cells, into hydrogen peroxide and aldehyde(s), thus inducing high cytotoxicity in cancer cells, was recently proposed. With the aim of improving the system efficacy by exploiting a nanotechnology approach, BSAO is covalently immobilized onto injectable nanohydrogels (NHs) based on cholesterol-graft-hyaluronic acid (HA-CH), a biocompatible conjugate that spontaneously leads to self-assembled structures in aqueous solutions. In this study, the physicochemical properties of the HA-CH-based NHs and the NHs cytocompatibility are reported. The properties of the NHs-BSAO system are also studied in terms of protein residual activity, both in vitro and on a model melanoma cell line.
authors.affiliation: Department of Drug Chemistry and Technologies, "Sapienza" University of Rome, P. le Aldo Moro 5, 00185, Rome, Italy.
authors.forename: Elita
authors.initials: E
authors.lastname: Montanari
authors.forename: Sabrina
authors.initials: S
authors.lastname: Capece
authors.forename: Chiara
authors.initials: C
authors.lastname: Di Meo
authors.forename: Martina
authors.initials: M
authors.lastname: Meringolo
authors.forename: Tommasina
authors.initials: T
authors.lastname: Coviello
authors.forename: Enzo
authors.initials: E
authors.lastname: Agostinelli
authors.forename: Pietro
authors.initials: P
authors.lastname: Matricardi
mesh_terms: D006631:Amine Oxidase (Copper-Containing) / Q000378:metabolism*; D000818:Animals; D002417:Cattle; D016923:Cell Death; D045744:Cell Line, Tumor; D002784:Cholesterol / Q000737:chemistry; D004800:Enzymes, Immobilized / Q000378:metabolism*; D006801:Humans; D006820:Hyaluronic Acid / Q000737:chemistry*; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry*; D008545:Melanoma / Q000188:drug therapy* / Q000201:enzymology*; D000080385:Nanogels; D010084:Oxidation-Reduction; D010316:Particle Size; D011092:Polyethylene Glycols / Q000737:chemistry*; D011094:Polyethyleneimine / Q000737:chemistry*; D055672:Static Electricity
chemical_list: D004800:Enzymes, Immobilized; D000080385:Nanogels; C413692:polyethylene glycol polyethyleneimine nanogel; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D011092:Polyethylene Glycols; D011094:Polyethyleneimine; D006820:Hyaluronic Acid; D002784:Cholesterol; D006631:Amine Oxidase (Copper-Containing)
keywords: bovine serum amine oxidase immobilization; hyaluronic acid; melanoma cancer cells; nanohydrogels; polyamines