title: Pathogen reduction treatment alters the immunomodulatory capacity of buffy coat-derived platelet concentrates.
abstract: BACKGROUND
  Storage of platelet concentrates (PCs) after Mirasol pathogen reduction technology (PRT) treatment changes platelet (PLT) surface marker expression and secretion of immunomodulatory factors. Given that PLTs are known to participate in immune function, PRT may alter the way PLTs interact with the immune cells of a recipient upon transfusion. As such, the aim of this study was to assess the effects of PRT treatment on the functional ability of PLTs to interact with peripheral blood mononuclear cells (PBMNCs).
  
  
  METHODS
  Buffy coat-derived PCs were pooled and split to obtain matched pairs. One unit was treated using the Mirasol PRT system, while the control PC remained untreated. After 5 days of storage, either the PLTs or the PLT supernatants from the PCs were cocultured with PBMNCs, with or without lipopolysaccharide (LPS). The immunomodulatory factors secreted into culture medium after coculture were examined.
  
  
  RESULTS
  PRT-treated PLTs and PLT supernatant significantly increased the interleukin (IL)-8 concentration, which was manifested only in the presence of LPS. Conversely, PRT-treated PLTs secreted less soluble P-selectin (sCD62P) upon coculture with PBMNCs.
  
  
  CONCLUSIONS
  PRT-treatment induced differential secretion of IL-8 and sCD62P during coculture, which may be attributed to either bioactive substances present in PLT supernatant or as a result of cell-cell interactions.
authors.affiliation: Research and Development, Australian Red Cross Blood Service, Sydney, Australia.
authors.forename: Yen S
authors.initials: YS
authors.lastname: Loh
authors.forename: Lacey
authors.initials: L
authors.lastname: Johnson
authors.forename: Matthew
authors.initials: M
authors.lastname: Kwok
authors.forename: Denese C
authors.initials: DC
authors.lastname: Marks
mesh_terms: D001792:Blood Platelets / Q000276:immunology / Q000378:metabolism*; D001793:Blood Preservation; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry; D006801:Humans; D016209:Interleukin-8 / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D019007:P-Selectin / Q000378:metabolism; D017713:Platelet Transfusion
chemical_list: D016209:Interleukin-8; D019007:P-Selectin