title: The UDP-N-acetylglucosamine:dolichol phosphate-N-acetylglucosamine-phosphotransferase gene as a new selection marker for potato transformation.
abstract: Here we describe the generation of potato plants that constitutively overexpressed, UDP-N-acetylglucosamine:dolichol phosphate-N-acetylglucosamine-phosphotransferase (GPT). Such transgenic plants can be formed in a medium with tunicamycin at 9.8 ± 0.28% efficiency, similar to the 9.4 ± 1.10 for the bialaphos resistance gene (Bar) gene. This study indicated that GPT transformation was very stable with high reproducibility, and that growth and tuber production in the GPT-transformed plants were stronger than in the wild-type plants.
authors.affiliation: Green-Bio Research Center, KRIBB, Daejeon 305-806, Korea.
authors.forename: Hyun-Soon
authors.initials: HS
authors.lastname: Kim
authors.forename: Mi-Sun
authors.initials: MS
authors.lastname: Kim
authors.forename: Kyoung-A
authors.initials: KA
authors.lastname: Hwang
authors.forename: Se-Won
authors.initials: SW
authors.lastname: Park
authors.forename: Jae-Heung
authors.initials: JH
authors.lastname: Jeon
mesh_terms: D005818:Genetic Engineering / Q000379:methods*; D005819:Genetic Markers / Q000235:genetics; D005822:Genetic Vectors / Q000235:genetics; D030821:Plants, Genetically Modified; D011198:Solanum tuberosum / Q000235:genetics*; D017855:Transferases (Other Substituted Phosphate Groups) / Q000235:genetics*; D014170:Transformation, Genetic
chemical_list: D005819:Genetic Markers; D017855:Transferases (Other Substituted Phosphate Groups); C023222:UDPacetylglucosamine-dolichyl-phosphate acetylglucosamine-1-phosphate transferase