title: Determination of pseudo-ginsenoside GQ in human plasma by high performance liquid chromatography-tandem mass spectrometry.
abstract: A specific, sensitive and rapid method based on high performance liquid chromatography coupled to tandem mass spectrometry (HPLC-MS/MS) was developed for the determination of pseudo-ginsenoside GQ in human plasma. Liquid-liquid extraction was used to isolate the analyte from biological matrix followed by injection of the extracts onto a C8 column with isocratic elution. Detection was carried out on a triple quadrupole tandem mass spectrometer (API-4000 system) in multiple reaction monitoring mode using negative electrospray ionization. The mobile phase consisted of methanol-10 mM ammonium acetate (90:10, v/v) and the flow rate was 0.3 mL/min. The method was validated over the concentration range of 5.0-5000.0 ng/mL for plasma. Inter- and intra-day precisions (relative standard deviation) were all within 15% and the accuracy (relative error) was ≤ 9.4%. The lower limit of quantitation was 5.0 ng/mL. The pseudo-ginsenoside GQ was stable after 8 h at room temperature, 24 h at autosampler and three freeze-thaw cycles (from -30 to 25 °C). The method was successfully applied to the pharmacokinetic study of pseudo-ginsenoside GQ in healthy Chinese volunteers.
authors.affiliation: Clinical Pharmacology Research Center, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100730, China.
authors.forename: Jiping
authors.initials: J
authors.lastname: Huo
authors.forename: Hongyun
authors.initials: H
authors.lastname: Wang
authors.forename: Pei
authors.initials: P
authors.lastname: Hu
authors.forename: Pingya
authors.initials: P
authors.lastname: Li
authors.forename: Jinping
authors.initials: J
authors.lastname: Liu
authors.forename: Ji
authors.initials: J
authors.lastname: Jiang
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D017321:Clinical Trials, Phase I as Topic; D004355:Drug Stability; D036145:Ginsenosides / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D006801:Humans; D016014:Linear Models; D016032:Randomized Controlled Trials as Topic; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D036145:Ginsenosides
keywords: LC-MS/MS; human plasma; pharmacokinetics; pseudo-ginsenoside GQ