title: Silk fibroin immobilization on poly(ethylene terephthalate) films: comparison of two surface modification methods and their effect on mesenchymal stem cells culture.
abstract: Silk fibroin (SF) has played a curial role for the surface modification of conventional materials to improve the biocompatibility, and SF modified poly(ethylene terephthalate) (PET) materials have potential applications on tissue engineering such as artificial ligament, artificial vessel, artificial heart valve sewing cuffs dacron and surgical mesh engineering. In this work, SF was immobilized onto PET film via two different methods: 1) plasma pretreatment followed by SF dip coating (PET-SF) and 2) plasma-induce acrylic acid graft polymerization and subsequent covalent immobilization of SF on PET film (PET-PAA-SF). It could be found that plasma treatment provided higher surface roughness which was suitable for further SF dip coating, while grafted poly(acrylic acid) (PAA) promised the covalent bonding between SF and PAA. ATR-FTIR adsorption band at 3284 cm(-1), 1623 cm(-1) and 1520 cm(-1) suggested the successful introduction of SF onto PET surface, while the amount of immobilized SF of PET-SF was higher than PET-PAA-SF according to XPS investigation (0.29 vs 0.23 for N/C ratio). Surface modified PET film was used as substrate for mesenchymal stem cells (MSCs) culture, the cells on PET-SF surface exhibited optimum density compared to PET-PAA-SF according to CCK-8 assays, which indicated that plasma pretreatment followed by SF dip coating was a simple and effective way to prepare biocompatible PET surface.
authors.affiliation: State Key Laboratory of Molecular Engineering of Polymers, Advanced Materials Laboratory, Department of Macromolecular Science, Fudan University, Shanghai 200433, PR China.
authors.forename: Meini
authors.initials: M
authors.lastname: Liang
authors.forename: Jinrong
authors.initials: J
authors.lastname: Yao
authors.forename: Xin
authors.initials: X
authors.lastname: Chen
authors.forename: Lei
authors.initials: L
authors.lastname: Huang
authors.forename: Zhengzhong
authors.initials: Z
authors.lastname: Shao
mesh_terms: D000818:Animals; D002452:Cell Count; D002478:Cells, Cultured; D005349:Fibroins / Q000494:pharmacology*; D055767:Immobilized Proteins / Q000494:pharmacology*; D008422:Materials Testing / Q000379:methods*; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000648:ultrastructure; D018625:Microscopy, Atomic Force; D056951:Photoelectron Spectroscopy; D011093:Polyethylene Terephthalates / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar; D017550:Spectroscopy, Fourier Transform Infrared; D013499:Surface Properties; D014867:Water / Q000737:chemistry
chemical_list: D055767:Immobilized Proteins; D011093:Polyethylene Terephthalates; D014867:Water; D005349:Fibroins
keywords: Cell culture; Covalent immobilization; Dip coating; Plasma treatment; Silk protein