title: Decreased retinal blood flow in experimental colitis; improvement by eye drop administration of losartan.
abstract: Patients with inflammatory bowel disease suffer not only from gut inflammation, but also from extraintestinal manifestations of the disease, including ocular pathology. The mechanisms causing ocular inflammation in these patients are unknown. The purpose of the current study was to investigate the possible vascular changes occurring in the retina using a mouse model of acute colitis, that is, ingestion of dextran sodium sulfate (DSS). Intravital microscopy of anesthetized mice revealed that DSS caused a significant 30-40% decrease in retinal red blood cell velocities, and a 45% decrease in total retinal blood flow, but no changes in intraocular pressure. To determine whether the decreases in retinal perfusion could be inhibited by an angiotensin II receptor antagonist, losartan was administered by eye drops in a subset of the mice prior to the intravital microscopy measurements. Topical losartan was able to largely attenuate the altered hemodynamics induced by DSS. We conclude that angiotensin II might be a possible target for reducing the vascular changes occurring distantly in the eye during colitis.
authors.affiliation: Department of Molecular and Cellular Physiology, Louisiana State University Health Sciences Center, 1501 Kings Highway, Shreveport, LA 71130, USA.
authors.forename: Megan N
authors.initials: MN
authors.lastname: Watts
authors.forename: Randa S
authors.initials: RS
authors.lastname: Eshaq
authors.forename: Patsy R
authors.initials: PR
authors.lastname: Carter
authors.forename: Norman R
authors.initials: NR
authors.lastname: Harris
mesh_terms: D000287:Administration, Topical; D047228:Angiotensin II Type 1 Receptor Blockers / Q000494:pharmacology*; D000809:Angiotensins / Q000097:blood; D000818:Animals; D001783:Blood Flow Velocity / Q000187:drug effects; D015153:Blotting, Western; D003092:Colitis / Q000139:chemically induced / Q000503:physiopathology*; D016264:Dextran Sulfate / Q000633:toxicity; D004195:Disease Models, Animal; D015590:Fluorophotometry; D019808:Losartan / Q000494:pharmacology*; D051379:Mice; D008810:Mice, Inbred C57BL; D009883:Ophthalmic Solutions; D012039:Regional Blood Flow / Q000187:drug effects; D012171:Retinal Vessels / Q000503:physiopathology*
chemical_list: D047228:Angiotensin II Type 1 Receptor Blockers; D000809:Angiotensins; D009883:Ophthalmic Solutions; D016264:Dextran Sulfate; D019808:Losartan
keywords: angiotensin II; colitis; dextran sodium sulfate; retinal perfusion