title: [Effect of estradiol on the expression of estrogen receptor in mandibular condylar cartilage cell of rat].
abstract: OBJECTIVE
  To evaluate the effect of estradiol on the expressions of estrogen receptor in mandibular condylar cartilage cell (MCC).
  
  
  METHODS
  The MCC were harvested from neonatal rats. The experimental groups was cultured in medium with different concentration(10(-3),10(-6),10(-9),l0(-12) mmol/L) of estradiol(E2), while the control group was cultured in medium with ethanol. The ERα mRNA and ERβ mRNA in cells were detected with RT-PCR.The data was processed with SPSS10.0 software package.
  
  
  RESULTS
  Both ERα and ERβ expression were observed in MCC. Exogenous 17β-E2 raised the gene expression of ERβ and lowered the expression of ERα. Under the concentration of 10-9 mmol/L(physiological condition), the up-regulation of ERα mRNA was found most obviously.
  
  
  CONCLUSIONS
  E2 can raise the expression of ERα and decrease the expression of ERβ, and the effect of up grading is the most obvious under physiological concentration.
authors.affiliation: Department of Oral and Maxillofacial Surgery, the Third Affiliated Hospital of Anhui Medical University, Hefei, Anhui Province, China. Crazy_ou@Hotmail.com
authors.forename: Lu
authors.initials: L
authors.lastname: Hong
authors.forename: Chuan-jun
authors.initials: CJ
authors.lastname: Chen
mesh_terms: D000818:Animals; D002356:Cartilage; D019902:Chondrocytes; D004958:Estradiol; D047628:Estrogen Receptor alpha; D047629:Estrogen Receptor beta; D008335:Mandibular Condyle; D012333:RNA, Messenger; D051381:Rats; D015533:Transcriptional Activation
chemical_list: D047628:Estrogen Receptor alpha; D047629:Estrogen Receptor beta; D012333:RNA, Messenger; D004958:Estradiol