title: Cytotoxic responses in BC3H1 myoblast cell lines exposed to 1-desulfoyessotoxin.
abstract: 1-Desulfoyessotoxin (1-dsYTX) is a desulfated polyether compound belonging to the yessotoxin group of marine toxins. This analogue has been detected in mussels. There are so far no reports on the mechanisms of action of 1-dsYTX in in vitro cell systems. This work evaluates cytotoxic responses in BC3H1 cells exposed to 100 nM 1-dsYTX. The toxicity of 1-dsYTX seems to be similar to that of yessotoxin (YTX). 1-Desulfoyessotoxin induced morphological and biochemical traits typical of a non-apoptotic form of cell death resembling paraptosis. Treated BC3H1 cells showed extensive cytoplasmic vacuolation, enlargement of mitochondria and endoplasmic reticulum and lack of DNA fragmentation. Western blotting analysis revealed phosphorylation of the protein kinase p38 and involvement of the heat shock protein Hsp70. This activation suggests involvement of different signalling pathways for programmed cell death.
authors.affiliation: The Norwegian School of Veterinary Science, P.O. Box 8146 Dep., N-0033 Oslo, Norway. monica.suarez.korsnes@nvh.no
authors.forename: Mónica Suárez
authors.initials: MS
authors.lastname: Korsnes
authors.forename: Arild
authors.initials: A
authors.lastname: Espenes
authors.forename: Lene C
authors.initials: LC
authors.lastname: Hermansen
authors.forename: Jared I
authors.initials: JI
authors.lastname: Loader
authors.forename: Christopher O
authors.initials: CO
authors.lastname: Miles
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D053938:DNA Fragmentation; D004988:Ethers, Cyclic / Q000633:toxicity*; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism; D051379:Mice; D008978:Mollusk Venoms / Q000633:toxicity*; D032446:Myoblasts / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D035964:Oxocins / Q000633:toxicity; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism
chemical_list: C401106:1-desulfoyessotoxin; D004988:Ethers, Cyclic; D018840:HSP70 Heat-Shock Proteins; D008978:Mollusk Venoms; D035964:Oxocins; D048051:p38 Mitogen-Activated Protein Kinases; C066632:yessotoxin
keywords: 1-Desulfoyessotoxin; Cytoplasmic vacuolation; DNA fragmentation; Endoplasmic reticulum; Hsp70; Intracellular signalling; Mitochondria; Paraptosis; Yessotoxin; p38