title: High-level production of calcium malate from glucose by Penicillium sclerotiorum K302.
abstract: In this study, after screening of 9 fungal strains for their ability to produce calcium malate, it was found that Penicillium sclerotiorum K302 among them could produce high-level of calcium malate. Under the optimal conditions, the titer of calcium malate in the supernatant was 88.6 g/l at flask level. During 10-l fermentation, the titer of 92.0 g/l, the yield of 0.88 g/g of glucose and the productivity of 1.23 g/l/h were reached within 72 h of the fermentation, demonstrating that the titer, yield and productivity of calcium malate by this strain were very high and the fermentation period was very short. After analysis of the partially purified product with HPLC, it was found that the main product was calcium malate. The results showed that P. sclerotiorum K302 obtained in this study was suitable for developing a novel one-step fermentation process for calcium malate production from glucose on large scale.
authors.affiliation: Unesco Chinese Center of Marine Biotechnology and Institute of Evolution and Marine Biodiversity, Ocean University of China, Yushan Road, No. 5, Qingdao, China.
authors.forename: Zhi-Peng
authors.initials: ZP
authors.lastname: Wang
authors.forename: Guang-Yuan
authors.initials: GY
authors.lastname: Wang
authors.forename: Ibrar
authors.initials: I
authors.lastname: Khan
authors.forename: Zhen-Ming
authors.initials: ZM
authors.lastname: Chi
mesh_terms: D001483:Base Sequence; D002118:Calcium / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D017931:DNA Primers; D005285:Fermentation; D005947:Glucose / Q000378:metabolism*; D008293:Malates / Q000737:chemistry / Q000378:metabolism*; D010407:Penicillium / Q000378:metabolism*; D016133:Polymerase Chain Reaction
chemical_list: D017931:DNA Primers; D008293:Malates; C030298:malic acid; D005947:Glucose; D002118:Calcium
keywords: Calcium malate; Fermentation; L-Malic acid; One step fermentation; P. sclerotiorum