title: [Bile duct reconstruction using 3-dimensional collagen tubes].
abstract: BACKGROUND
  In recent years, with widespread laparoscopic cholecystectomy and liver transplantation, complications involving the biliary system are increasing. All current techniques have a high risk of recurrence or high-morbidity.
  
  
  METHODS
  A 3-dimensional collagen bile duct modified with agarose hydrogel was developed to substitute the affected extrahepatic bile duct. It was used in 40 guinea pigs and the histology and physiology was studied at 4 weeks, 3 and 6 months after transplantation.
  
  
  CONCLUSIONS
  The graft shows to have a high potential in applications to treat hepatobiliary diseases which require surgery.
authors.affiliation: Departamento de Cirugía Experimental, Universidad de Granada, Granada, España. Electronic address: apma85@hotmail.com.
authors.forename: Alejandro José
authors.initials: AJ
authors.lastname: Pérez Alonso
authors.forename: Carlos
authors.initials: C
authors.lastname: del Olmo Rivas
authors.forename: Ignacio
authors.initials: I
authors.lastname: Machado Romero
authors.forename: Beatriz
authors.initials: B
authors.lastname: Pérez Cabrera
authors.forename: Francisco Javier
authors.initials: FJ
authors.lastname: Cañizares Garcia
authors.forename: Pablo
authors.initials: P
authors.lastname: Torne Poyatos
mesh_terms: D000818:Animals; D017734:Bile Ducts, Extrahepatic / Q000601:surgery*; D001662:Biliary Tract Surgical Procedures / Q000379:methods; D001672:Biocompatible Materials; D003094:Collagen; D006168:Guinea Pigs; D019736:Prostheses and Implants; D011474:Prosthesis Design
chemical_list: D001672:Biocompatible Materials; D003094:Collagen
keywords: Bile duct; Ingeniería tisular; New techniques; Nuevas técnicas; Repair; Reparación; Tissue engineering; Vía biliar