title: Fluorescence-activated cell sorting and directed evolution of α-N-acetylgalactosaminidases using a quenched activity-based probe (qABP).
abstract: An α-N-acetylgalactosamine-containing quenched activity-based probe (qABP) was designed and successfully synthesized, and it was subsequently used in directed enzyme evolution experiments aided by fluorescence-activated cell sorting (FACS) for the discovery of an α-N-acetylgalactosaminidase variant with improved catalytic activity.
authors.affiliation: Defence Medical and Environmental Research Institute, DSO National Laboratories, 27 Medical Drive, Singapore 117510, Singapore.
authors.forename: Kamaladasan
authors.initials: K
authors.lastname: Kalidasan
authors.forename: Ying
authors.initials: Y
authors.lastname: Su
authors.forename: Xiaoyuan
authors.initials: X
authors.lastname: Wu
authors.forename: Shao Q
authors.initials: SQ
authors.lastname: Yao
authors.forename: Mahesh
authors.initials: M
authors.lastname: Uttamchandani
mesh_terms: D019020:Directed Molecular Evolution; D041965:Flavobacteriaceae / Q000201:enzymology; D005434:Flow Cytometry; D005456:Fluorescent Dyes / Q000737:chemistry*; D007700:Kinetics; D009154:Mutation; D048809:alpha-N-Acetylgalactosaminidase / Q000235:genetics / Q000378:metabolism*
chemical_list: D005456:Fluorescent Dyes; D048809:alpha-N-Acetylgalactosaminidase