title: Structurally conserved and functionally divergent yeast Ssu72 phosphatases.
abstract: The eukaryotic Ssu72 factor is involved in several RNA biogenesis processes. It has phosphatase activity on the carboxy-terminal domain (CTD) of the major subunit of RNA polymerase II. The Kluyveromyces lactis Ssu72 (KlSsu72) shows in vitro phosphatase activity for the pNPP substrate, and this activity is inhibited by ortho-vanadate. The expression of KlSsu72 in Saccharomyces cerevisiae shows defective CTD serine5-P phosphatase activity and reveals the importance of Ssu72 for the normal CTD serine5-P levels at two growth states. The divergence is emphasised by the remarkable changes in RNA14 alternative 3'-end RNA processing, which are independent of the CTD serine5-P levels.
authors.affiliation: Area de Bioquímica, Facultad de Ciencias, Universidade da Coruña (UDC), A Coruña, Spain.
authors.forename: Ana M
authors.initials: AM
authors.lastname: Rodríguez-Torres
authors.forename: Mónica
authors.initials: M
authors.lastname: Lamas-Maceiras
authors.forename: Rosa
authors.initials: R
authors.lastname: García-Díaz
authors.forename: María A
authors.initials: MA
authors.lastname: Freire-Picos
mesh_terms: D000595:Amino Acid Sequence; D020134:Catalytic Domain; D017124:Conserved Sequence; D007716:Kluyveromyces / Q000378:metabolism*; D008969:Molecular Sequence Data; D010749:Phosphoprotein Phosphatases / Q000737:chemistry*; D010766:Phosphorylation; D011485:Protein Binding; D012313:RNA / Q000032:analysis / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry*; D017386:Sequence Homology, Amino Acid; D014158:Transcription, Genetic; D014638:Vanadates / Q000494:pharmacology; D039221:mRNA Cleavage and Polyadenylation Factors / Q000737:chemistry*
chemical_list: C416934:SSU72 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D039221:mRNA Cleavage and Polyadenylation Factors; D014638:Vanadates; D012313:RNA; D010749:Phosphoprotein Phosphatases; C116022:carboxy-terminal domain phosphatase
keywords: 5-fluoro-orotic acid; CTD; CTD-phosphatase; FOA; GST; OD(600); RNA polymerase II; RNA polymerase-II; RNA-processing; RNAPII; SSU72; Ser2, Ser5; Yeast; carboxy-terminal domain (CTD) of the major subunit of RNA polymerase II; glutathione S-transferase; optical density at 600 nanometers; pNPP; paranitrophenylphosphate; serine 2 and 5, respectively, from CTD repeats