title: Anti-inflammatory activity of different agave plants and the compound cantalasaponin-1.
abstract: Species of the agave genus, such as Agave tequilana, Agave angustifolia and Agave americana are used in Mexican traditional medicine to treat inflammation-associated conditions. These plants' leaves contain saponin compounds which show anti-inflammatory properties in different models. The goal of this investigation was to evaluate the anti-inflammatory capacity of these plants, identify which is the most active, and isolate the active compound by a bio-directed fractionation using the ear edema induced in mice with 12-O-tetradecanoylphorbol-13-acetate (TPA) technique. A dose of 6 mg/ear of acetone extract from the three agave species induced anti-inflammatory effects, however, the one from A. americana proved to be the most active. Different fractions of this species showed biological activity. Finally the F5 fraction at 2.0 mg/ear induced an inhibition of 85.6%. We identified one compound in this fraction as (25R)-5α-spirostan-3β,6α,23α-triol-3,6-di-O-β-D-glucopyranoside (cantalasaponin-1) through 1H- and 13C-NMR spectral analysis and two dimensional experiments like DEPT NMR, COSY, HSQC and HMBC. This steroidal glycoside showed a dose dependent effect of up to 90% of ear edema inhibition at the highest dose of 1.5 mg/ear.
authors.affiliation: Centro de Investigación Biomédica del Sur (CIBIS), Instituto Mexicano del Seguro Social (IMSS), Argentina No. 1, Col. Centro, Xochitepec 62790, Morelos, Mexico. monterrosasnayeli@hotmail.com
authors.forename: Nayeli
authors.initials: N
authors.lastname: Monterrosas-Brisson
authors.forename: Martha L Arenas
authors.initials: ML
authors.lastname: Ocampo
authors.forename: Enrique
authors.initials: E
authors.lastname: Jiménez-Ferrer
authors.forename: Antonio R
authors.initials: AR
authors.lastname: Jiménez-Aparicio
authors.forename: Alejandro
authors.initials: A
authors.lastname: Zamilpa
authors.forename: Manases
authors.initials: M
authors.lastname: Gonzalez-Cortazar
authors.forename: Jaime
authors.initials: J
authors.lastname: Tortoriello
authors.forename: Maribel
authors.initials: M
authors.lastname: Herrera-Ruiz
mesh_terms: D027882:Agave / Q000737:chemistry*; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000032:analysis / Q000737:chemistry / Q000494:pharmacology*; D004487:Edema / Q000188:drug therapy*; D008297:Male; D008519:Medicine, Traditional; D051379:Mice; D008813:Mice, Inbred ICR; D018515:Plant Leaves / Q000378:metabolism; D028321:Plant Preparations / Q000494:pharmacology; D012503:Saponins / Q000032:analysis / Q000737:chemistry / Q000494:pharmacology*; D013755:Tetradecanoylphorbol Acetate
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D028321:Plant Preparations; D012503:Saponins; C586220:cantalasaponin-1; D013755:Tetradecanoylphorbol Acetate