title: Functional expression of thyroid-stimulating hormone receptor on nano-sized bacterial magnetic particles in Magnetospirillum magneticum AMB-1.
abstract: The measurement of autoantibodies to thyroid-stimulating hormone receptor (TSHR) is important for the diagnosis of autoimmune thyroid disease such as Graves' disease (GD). Although TSHR from porcine thyroid membrane is commonly used for the measurement of TSHR autoantibodies (TRAb), recombinant human TSHR (hTSHR) remains ideal in terms of stable supply and species identity. Here we set out to express recombinant hTSHR on the lipid-bilayer surface of magnetic nanoparticles from a magnetotactic bacterium, Magnetospirillum magneticum AMB-1. Using a tetracycline-inducible expression system, we successfully overexpressed functional hTSHR on bacterial magnetic particles (BacMPs) in AMB-1 via an anchor protein specific for BacMPs. The overexpressed hTSHR was membrane integrated and possessed both ligand and autoantibody binding activity. Our data suggest that hTSHR-displayed BacMPs have potential as novel tools for ligand-receptor interaction analysis or for TRAb immunoassay in GD patients.
authors.affiliation: Division of Biotechnology and Life Science, Institute of Engineering, Tokyo University of Agriculture and Technology, 2-24-16, Naka-cho, Koganei, Tokyo 184-8588, Japan. 50011702104@st.tuat.ac.jp
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Sugamata
authors.forename: Ryo
authors.initials: R
authors.lastname: Uchiyama
authors.forename: Toru
authors.initials: T
authors.lastname: Honda
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Tanaka
authors.forename: Tadashi
authors.initials: T
authors.lastname: Matsunaga
authors.forename: Tomoko
authors.initials: T
authors.lastname: Yoshino
mesh_terms: D001323:Autoantibodies / Q000276:immunology; D006111:Graves Disease / Q000175:diagnosis / Q000276:immunology / Q000378:metabolism; D006801:Humans; D008051:Lipid Bilayers / Q000378:metabolism; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D042201:Magnetospirillum / Q000378:metabolism*; D010957:Plasmids / Q000235:genetics / Q000378:metabolism; D011989:Receptors, Thyrotropin / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology
chemical_list: D001323:Autoantibodies; D008051:Lipid Bilayers; D058185:Magnetite Nanoparticles; D011989:Receptors, Thyrotropin; D011994:Recombinant Proteins