title: Injectable extracellular matrix hydrogel developed using porcine articular cartilage.
abstract: This work was first development of a delivery system capable of maintaining a sustained release of protein drugs at specific sites by using potentially biocompatible porcine articular cartilage. The prepared porcine articular cartilage powder (PCP) was easily soluble in phosphate-buffered saline. The PCP suspension easily entrapped bovine serum albumin-fluorescein isothiocyanate (BSA-FITC) in pharmaceutical formulations at room temperature. The aggregation of PCP and BSA-FITC was confirmed by dynamic light scattering. When the BSA-FITC-loaded PCP suspension was subcutaneously injected into rats, it gelled and formed an interconnecting three-dimensional PCP structure that allowed BSA to penetrate through it. The amount of BSA-FITC released from the PCP hydrogel was determined in rat plasma and monitored by real-time in vivo molecular imaging. The data indicated sustained release of BSA-FITC for 20 days in vivo. In addition, the PCP hydrogel induced a slight inflammatory response. In conclusion, we showed that the PCP hydrogel could serve as a minimally invasive therapeutics depot.
authors.affiliation: Department of Molecular Science and Technology, Ajou University, Suwon 443-749, Republic of Korea.
authors.forename: Jin Seon
authors.initials: JS
authors.lastname: Kwon
authors.forename: So Mi
authors.initials: SM
authors.lastname: Yoon
authors.forename: Sun Woo
authors.initials: SW
authors.lastname: Shim
authors.forename: Ji Hoon
authors.initials: JH
authors.lastname: Park
authors.forename: Kyung Jun
authors.initials: KJ
authors.lastname: Min
authors.forename: Hyun Ju
authors.initials: HJ
authors.lastname: Oh
authors.forename: Jae Ho
authors.initials: JH
authors.lastname: Kim
authors.forename: Young Jick
authors.initials: YJ
authors.lastname: Kim
authors.forename: Jun Jin
authors.initials: JJ
authors.lastname: Yoon
authors.forename: Byung Hyune
authors.initials: BH
authors.lastname: Choi
authors.forename: Moon Suk
authors.initials: MS
authors.lastname: Kim
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000008:administration & dosage / Q000737:chemistry* / Q000633:toxicity; D002358:Cartilage, Articular / Q000737:chemistry*; D003692:Delayed-Action Preparations; D004337:Drug Carriers; D005109:Extracellular Matrix / Q000737:chemistry*; D016650:Fluorescein-5-isothiocyanate / Q000008:administration & dosage / Q000031:analogs & derivatives / Q000737:chemistry; D020100:Hydrogels; D007249:Inflammation / Q000139:chemically induced; D007279:Injections, Subcutaneous; D008027:Light; D011208:Powders; D051381:Rats; D017207:Rats, Sprague-Dawley; D012542:Scattering, Radiation; D012710:Serum Albumin, Bovine / Q000008:administration & dosage / Q000737:chemistry; D012995:Solubility; D013678:Technology, Pharmaceutical / Q000379:methods; D013696:Temperature; D013997:Time Factors; D014783:Viscosity
chemical_list: D001672:Biocompatible Materials; D003692:Delayed-Action Preparations; D004337:Drug Carriers; D020100:Hydrogels; D011208:Powders; C039217:fluorescein isothiocyanate bovine serum albumin; D012710:Serum Albumin, Bovine; D016650:Fluorescein-5-isothiocyanate
keywords: BSA; Hydrogel; Injectable; Porcine cartilage powder; Protein