title: The Saccharomyces cerevisiae transcriptome as a mirror of phytochemical variation in complex extracts of Equisetum arvense from America, China, Europe and India.
abstract: BACKGROUND
  Pattern-oriented chemical profiling is increasingly being used to characterize the phytochemical composition of herbal medicines for quality control purposes. Ideally, a fingerprint of the biological effects should complement the chemical fingerprint. For ethical and practical reasons it is not possible to test each herbal extract in laboratory animals or humans. What is needed is a test system consisting of an organism with relevant biology and complexity that can serve as a surrogate in vitro system. The purpose of this study was to test the hypothesis that the Saccharomyces cerevisiae transcriptome might be used as an indicator of phytochemical variation of closely-related yet distinctly different extracts prepared from a single species of a phytogeographically widely distributed medicinal plant. We combined phytochemical profiling using chromatographic methods (HPTLC, HPLC-PDA-MS/MS) and gene expression studies using Affymetrix Yeast 2.0 gene chip with principal component analysis and k-nearest neighbor clustering analysis to test this hypothesis using extracts prepared from the phytogeographically widely distributed medicinal plant Equisetum arvense as a test case.
  
  
  RESULTS
  We found that the Equisetum arvense extracts exhibited qualitative and quantitative differences in their phytochemical composition grouped along their phytogeographical origin. Exposure of yeast to the extracts led to changes in gene expression that reflected both the similarities and differences in the phytochemical composition of the extracts. The Equisetum arvense extracts elicited changes in the expression of genes involved in mRNA translation, drug transport, metabolism of energy reserves, phospholipid metabolism, and the cellular stress response.
  
  
  CONCLUSIONS
  Our data show that functional genomics in S. cerevisiae may be developed as a sensitive bioassay for the scientific investigation of the interplay between phytochemical composition and transcriptional effects of complex mixtures of chemical compounds. S. cerevisiae transcriptomics may also be developed for testing of mixtures of conventional drugs ("polypills") to discover novel antagonistic or synergistic effects of those drug combinations.
authors.affiliation: Centre for Complementary Medicine Research, University of Western Sydney, Locked Bag 1797, Penrith, NSW 2751, Australia.
authors.forename: Rebekah
authors.initials: R
authors.lastname: Cook
authors.forename: James R
authors.initials: JR
authors.lastname: Hennell
authors.forename: Samiuela
authors.initials: S
authors.lastname: Lee
authors.forename: Cheang S
authors.initials: CS
authors.lastname: Khoo
authors.forename: Maria C
authors.initials: MC
authors.lastname: Carles
authors.forename: Vincent J
authors.initials: VJ
authors.lastname: Higgins
authors.forename: Suresh
authors.initials: S
authors.lastname: Govindaraghavan
authors.forename: Nikolaus J
authors.initials: NJ
authors.lastname: Sucher
mesh_terms: D000569:Americas; D002681:China; D030541:Databases, Genetic; D020829:Equisetum / Q000737:chemistry*; D005060:Europe; D016166:Free Radical Scavengers / Q000737:chemistry / Q000494:pharmacology; D007194:India; D020411:Oligonucleotide Array Sequence Analysis; D010936:Plant Extracts / Q000737:chemistry* / Q000494:pharmacology*; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000235:genetics*; D059467:Transcriptome / Q000187:drug effects*
chemical_list: D016166:Free Radical Scavengers; D010936:Plant Extracts