title: MiR-25-3p attenuates the proliferation of tongue squamous cell carcinoma cell line Tca8113.
abstract: OBJECTIVE
  To investigate the effects of miR-25-3p on the occurrence, development and proliferation of tongue squamous cell carcinoma cells.
  
  
  METHODS
  To establish tongue squamous cell carcinoma cell line Tca8113 that stably and highly express miR-25-3p using recombinant retroviral vector-mediated gene transfer method. The proliferation of transfected Tca8113 was detected by thiazolyl blue tetrazolium bromide (MTT) and cell colony formation assays. cyclinD1, p21(cip1) and p27(kip1) mRNA expressions in the transfected Tca-8113 were detected by quantitative PCR. cyclinD1, p21, p27(kip1), AKT, p-AKT, FOXO1 and p-FOXO1 expressions in the transfected Tca8113 were detected by western blot analysis. In addition, miR-25-3p expression in the tongue squamous cell carcinoma cell line and tissue specimen was also detected by quantitative PCR.
  
  
  RESULTS
  Quantitative PCR showed that miR-25-3p expression in the tongue squamous cell carcinoma cell lines and tissue specimen was significantly lower than that in the adjacent tissue. MTT and cell colony formation assays showed that after miR-25-3p overexpression, the proliferation of transfected Tca8113 was obviously attenuated. Western blot analysis and quantitative PCR showed that after miR-25-3p overexpression, p21(cip1) and p27(kip1) expressions were upregulated, while cyclinD1, AKT, FOXO1 expressions were downregulated, and AKT and FOXO1 phosphorylation was inactivated in the transfected Tca8113 cells.
  
  
  CONCLUSIONS
  MiR-25-3p inhibited the proliferation of tongue squamous cell carcinoma cells and regulated cell cycle-related protein expression, playing an important role in the occurrence and development of squamous cell carcinoma of the tongue.
authors.affiliation: Department of Stomatology, Zhoupu Hospital of Shanghai Pudong New District, Shanghai 201318, China.
authors.forename: Jia-Ying
authors.initials: JY
authors.lastname: Xu
authors.forename: Li-Li
authors.initials: LL
authors.lastname: Yang
authors.forename: Chao
authors.initials: C
authors.lastname: Ma
authors.forename: Yuan-Liang
authors.initials: YL
authors.lastname: Huang
authors.forename: Gui-Xiang
authors.initials: GX
authors.lastname: Zhu
authors.forename: Qi-Lin
authors.initials: QL
authors.lastname: Chen
mesh_terms: D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000378:metabolism / Q000503:physiopathology*; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015536:Down-Regulation; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D014062:Tongue Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000503:physiopathology*; D014162:Transfection
chemical_list: D018797:Cell Cycle Proteins; C543351:MIRN25 microRNA, human; D035683:MicroRNAs
keywords: AKT/FOXO1; Cellular proliferation; MiR-25-3p; Retrovirus; Stable cell line; Tongue squamous cell carcinoma