title: The influence of conjugates isolated from Matricaria chamomilla L. on platelets activity and cytotoxicity.
abstract: Cardiovascular diseases (CVD) remain the principal cause of death in both advanced and developing countries of the world. Blood platelets are involved in the pathogenesis of atherosclerosis and thrombosis. Platelet adhesion and aggregation are critical events that occur in unstable coronary syndromes. The current research is focused on the role of polysaccharide-polyphenolic conjugates isolated from chamomile (Matricaria chamomilla L.) at concentrations of 10, 25, 50 and 100 μg/mL on blood platelets (obtained from healthy donors and from patients received combined anti-platelet therapy complex with clopidogrel and acetylsalicylic acid) aggregation and experimentally induced cell toxicity. The treatment of PRP obtained from healthy donors with polyphenolic-polysaccharide conjugates from M. chamomilla (L.) (MC) resulted in a dose-dependent, decrease of platelet aggregation induced by multiple agonists (ADP, collagen and arachidonic acid). In this study we also observed that the MC reduced platelet aggregation in PRP obtained from patients with cardiovascular disorders. The result of testing the MC on human blood platelets, mouse fibroblast cultures L929 and human lung cells A549 did not show any cytotoxicity effects. Compounds obtained from M. chamomilla L. are potential composite to the development of a new anti-platelet agent, which could be an alternative to the currently used anti-platelet drugs.
authors.affiliation: Department of General Biochemistry, Faculty of Biology and Environmental Protection, University of Lodz, Pomorska 141/143, 90-236 Lodz, Poland; Regional Specialist Hospital in Wroclaw, Research and Development Centre, H.M. Kamieńskiego 73a, 51-124 Wroclaw, Poland. Electronic address: mbijak@biol.uni.lodz.pl.
authors.forename: Michał
authors.initials: M
authors.lastname: Bijak
authors.forename: Joanna
authors.initials: J
authors.lastname: Saluk
authors.forename: Marta
authors.initials: M
authors.lastname: Tsirigotis-Maniecka
authors.forename: Halina
authors.initials: H
authors.lastname: Komorowska
authors.forename: Barbara
authors.initials: B
authors.lastname: Wachowicz
authors.forename: Ewa
authors.initials: E
authors.lastname: Zaczyńska
authors.forename: Anna
authors.initials: A
authors.lastname: Czarny
authors.forename: Franciszek
authors.initials: F
authors.lastname: Czechowski
authors.forename: Paweł
authors.initials: P
authors.lastname: Nowak
authors.forename: Izabela
authors.initials: I
authors.lastname: Pawlaczyk
mesh_terms: D000818:Animals; D001792:Blood Platelets / Q000187:drug effects*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D006801:Humans; D031168:Matricaria / Q000737:chemistry*; D051379:Mice; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D010974:Platelet Aggregation / Q000187:drug effects; D059808:Polyphenols / Q000737:chemistry / Q000494:pharmacology* / Q000633:toxicity; D011134:Polysaccharides / Q000737:chemistry / Q000494:pharmacology* / Q000633:toxicity; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D010936:Plant Extracts; D059808:Polyphenols; D011134:Polysaccharides
keywords: Anti-platelet activity; Experimental cytotoxicity; Matricaria chamomilla L.