title: Identification of Novel Stress Granule Components That Are Involved in Nuclear Transport.
abstract: Importin-α1 belongs to a subfamily of nuclear transport adaptors and participates in diverse cellular functions. Best understood for its role in protein transport, importin-α1 also contributes to other biological processes. For instance, arsenite treatment causes importin-α1 to associate with cytoplasmic stress granules (SGs) in mammalian cells. These stress-induced compartments contain translationally arrested mRNAs, small ribosomal subunits and numerous proteins involved in mRNA transport and metabolism. At present, it is not known whether members of all three importin-α subfamilies locate to SGs in response to stress.
  
  
  
  Here, we demonstrate that the oxidant diethyl maleate (DEM), arsenite and heat shock, promote the formation of cytoplasmic SGs that contain nuclear transport factors. Specifically, importin-α1, α4 and α5, which belong to distinct subfamilies, and importin-β1 were targeted by all of these stressors to cytoplasmic SGs, but not to P-bodies. Importin-α family members have been implicated in transcriptional regulation, which prompted us to analyze their ability to interact with poly(A)-RNA in growing cells. Our studies show that importin-α1, but not α4, α5, importin-β1 or CAS, associated with poly(A)-RNA under nonstress conditions. Notably, this interaction was significantly reduced when cells were treated with DEM. Additional studies suggest that importin-α1 is likely connected to poly(A)-RNA through an indirect interaction, as the adaptor did not bind homopolymer RNA specifically in vitro.
  
  
  
  Our studies establish that members of three importin-α subfamilies are bona fide SG components under different stress conditions. Furthermore, importin-α1 is unique in its ability to interact with poly(A)-RNA in a stress-dependent fashion, and in vitro experiments indicate that this association is indirect. Collectively, our data emphasize that nuclear transport factors participate in a growing number of cellular activities that are modulated by stress.
authors.affiliation: Department of Physiology, McGill University, Montreal, Quebec, Canada.
authors.forename: Hicham
authors.initials: H
authors.lastname: Mahboubi
authors.forename: Evangeline
authors.initials: E
authors.lastname: Seganathy
authors.forename: Dekun
authors.initials: D
authors.lastname: Kong
authors.forename: Ursula
authors.initials: U
authors.lastname: Stochaj
mesh_terms: D021581:Active Transport, Cell Nucleus / Q000502:physiology*; D018053:Arsenites / Q000378:metabolism; D015153:Blotting, Western; D003593:Cytoplasm / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D006367:HeLa Cells; D018869:Heat-Shock Response / Q000502:physiology*; D006801:Humans; D008298:Maleates / Q000378:metabolism; D018384:Oxidative Stress / Q000502:physiology*; D028901:alpha Karyopherins / Q000378:metabolism; D028961:beta Karyopherins / Q000378:metabolism
chemical_list: D018053:Arsenites; D008298:Maleates; D028901:alpha Karyopherins; D028961:beta Karyopherins; C014476:diethyl maleate; C015001:arsenite