title: Ethanol extract of Cordyceps militaris grown on germinated soybeans attenuates dextran-sodium-sulfate- (DSS-) induced colitis by suppressing the expression of matrix metalloproteinases and inflammatory mediators.
abstract: The effect of Cordyceps militaris (CM) grown on germinated soybeans (GSC) in the inflammatory bowel disease (IBD) model was studied. To demonstrate the preventive effect of GSC extract in a dextran-sodium-sulfate- (DSS-) induced acute colitis mouse model, GSC was administered 2 days before DSS coadministration. GSC significantly suppressed DSS-induced disease activity index (DAI) as well as histopathological scores, compared to control or CM-treated group. To elucidate the anti-IBD activity of GSC, we checked the level of matrix metalloproteinases (MMPs) and inflammatory mediators. GSC extract decreased the level of MMP-3 and -9 mRNAs and p53 proteins. The level and activity of LPS-induced MMP-9 were reduced in GSC-treated RAW264.7 cells. It also attenuated the level of inducible nitric oxide synthase (iNOS) and tumor necrosis factor- (TNF-) α mRNAs both in colon tissue and in macrophage cells. These results suggest that GSC can be applied as a protective agent against IBDs.
authors.affiliation: Department of Bioscience and Biotechnology, Konkuk University, 1 Hwayang-dong, Gwangjin-gu, Seoul 143-701, Republic of Korea.
authors.forename: Dong Ki
authors.initials: DK
authors.lastname: Park
authors.forename: Hye-Jin
authors.initials: HJ
authors.lastname: Park
mesh_terms: D000818:Animals; D003092:Colitis / Q000139:chemically induced / Q000188:drug therapy*; D032661:Cordyceps / Q000737:chemistry / Q000254:growth & development; D016264:Dextran Sulfate / Q000633:toxicity; D004195:Disease Models, Animal; D006801:Humans; D007249:Inflammation / Q000139:chemically induced / Q000188:drug therapy; D015212:Inflammatory Bowel Diseases / Q000139:chemically induced / Q000188:drug therapy*; D019278:Matrix Metalloproteinase 3 / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D051379:Mice; D010936:Plant Extracts / Q000008:administration & dosage* / Q000737:chemistry; D013025:Glycine max / Q000737:chemistry / Q000254:growth & development / Q000382:microbiology; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism
chemical_list: D010936:Plant Extracts; D016159:Tumor Suppressor Protein p53; D016264:Dextran Sulfate; D019278:Matrix Metalloproteinase 3; D020780:Matrix Metalloproteinase 9