title: An adapted duck Tembusu virus induces systemic infection and mediates antibody-dependent disease severity in mice.
abstract: Duck Tembusu virus (DTMUV) is a newly emerging infectious agent in China. Since 2010, this presumably arthropod-borne virus has caused severe disease in duck flocks. A cell-adapted DTMUV, designated AH10, has been developed. For this particular virus, replication, pathogenicity, and antibody-dependent enhancement (ADE) infection mediated by a specific antibody was investigated in mammalian cells and mice. The virus propagated in Vero or Hepa1-6 cells with a stable titer, and induced a visible cytopathic effect. Expression of pro- and anti-inflammatory cytokine genes in Hepa1-6 cells was elevated during early infection stages. AH10 was able to replicate in the brain, spleen, liver and kidneys of mice, and resulted in a systemic infection by day 5 when administered intracerebrally. When naïve mice were first transfused intraperitoneally with virus-specific antisera, upon subsequent infection, ADE of virus symptoms was observed. Mice displayed clinical symptoms by day 3 post-infection; weight loss in the AH10-specific antibody-treated group was observed 2-3 days earlier than that in the control groups. These findings indicate that AH10 was able to replicate in the mouse model, and that anti-AH10 antibodies caused ADE. The molecular basis of pathogenicity and ADE in the mouse model should be investigated in the future.
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, PR China.
authors.forename: Zongliang
authors.initials: Z
authors.lastname: Liu
authors.forename: Yanhong
authors.initials: Y
authors.lastname: Ji
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Huang
authors.forename: Yuguang
authors.initials: Y
authors.lastname: Fu
authors.forename: Jianzhong
authors.initials: J
authors.lastname: Wei
authors.forename: Xuepeng
authors.initials: X
authors.lastname: Cai
authors.forename: Qiyun
authors.initials: Q
authors.lastname: Zhu
mesh_terms: D000825:Animal Structures / Q000821:virology; D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology*; D019067:Antibody-Dependent Enhancement; D002460:Cell Line; D002522:Chlorocebus aethiops; D016207:Cytokines / Q000096:biosynthesis; D003588:Cytopathogenic Effect, Viral; D004195:Disease Models, Animal; D005260:Female; D005416:Flavivirus / Q000276:immunology*; D018177:Flavivirus Infections / Q000276:immunology* / Q000473:pathology*; D020869:Gene Expression Profiling; D054884:Host-Pathogen Interactions; D051379:Mice; D008807:Mice, Inbred BALB C; D019562:Viral Load
chemical_list: D000914:Antibodies, Viral; D016207:Cytokines
keywords: Antibody-dependent enhancement; Duck Tembusu virus; Mouse model; Pathogenicity; Replication