title: [Expression of NA of influenza virus and C3d fusion gene in replication-defective recombinant adenovirus and its immune efficacy analysis].
abstract: OBJECTIVE
  To construct a replication-defective recombinant adenovirus expressing the fusion gene of neuraminidase (NA) gene in influenza virus A/FM/1/47 and C3d and to evaluate the induced immune efficacy.
  
  
  METHODS
  NA-C3d was cloned into shutter vector pAdTrack-CMV, which was cotransformated with adenovirus DNA into E. coli BJ5183. The recombinant adenovirus genomic DNA was generated through homological recombination. The recombinant adenovirus was produced by transfecting 293 cell line with the genomic DNA and the induced immune efficacy in mice were analyzed.
  
  
  RESULTS
  The integration of NA-C3d in the adenovirus genomic DNA and its expression were confirmed by PCR and Western-Blot assays respectively. After intranasal immunization, the serum IgG was induced at a titer of 1: 1000 and 1:100 000 in BALB/c mice at primary and secondary immunization respectively. The vaccinated mice were completely survived when challenged with wide influenza virus.
  
  
  CONCLUSIONS
  recombinant adenovirus expressing NA-C3d was successfully constructed and it could induce desired immune efficacy.
authors.affiliation: Chinese Center for Disease Control and Prevention, Beijing, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Han
authors.forename: Xuan
authors.initials: X
authors.lastname: Wang
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.forename: Xiao-dong
authors.initials: XD
authors.lastname: Zhao
authors.forename: Shu-hua
authors.initials: SH
authors.lastname: Wu
mesh_terms: D000256:Adenoviridae / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D000818:Animals; D003001:Cloning, Molecular; D015933:Complement C3d / Q000096:biosynthesis* / Q000235:genetics; D005822:Genetic Vectors / Q000235:genetics; D007074:Immunoglobulin G / Q000276:immunology; D029521:Alphainfluenzavirus / Q000201:enzymology* / Q000235:genetics*; D051379:Mice; D008807:Mice, Inbred BALB C; D009439:Neuraminidase / Q000096:biosynthesis* / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis* / Q000235:genetics; D014162:Transfection / Q000379:methods; D014779:Virus Replication
chemical_list: D007074:Immunoglobulin G; D011993:Recombinant Fusion Proteins; D015933:Complement C3d; D009439:Neuraminidase