title: High-yield expression of mouse Aos1-Uba2-fusion SUMO-activating enzyme, mAU, in a baculovirus-insect cell system.
abstract: Small ubiquitin-related modifier (SUMO) research requires large amounts of SUMO-activating enzyme (SUMO-E1) for in vitro SUMOylation assays. In this study the expression and purification of a hexahistidine-tagged mouse Aos1-Uba2-fusion protein (His6-mAU) in a baculovirus-insect cell system revealed that approximately 1.0 mg of highly-purified His6-mAU was obtained from 100 mL of Sf9 cell culture. The recombinant protein had SUMO-E1 activity in multiple in vitro SUMOylation assays.
authors.affiliation: Faculty of Sciences, Kumamoto University, Kumamoto 860-8555, Japan.
authors.forename: Ayumi
authors.initials: A
authors.lastname: Kanemaru
authors.forename: Hisato
authors.initials: H
authors.lastname: Saitoh
mesh_terms: D000818:Animals; D016367:Baculoviridae / Q000235:genetics*; D015870:Gene Expression; D005818:Genetic Engineering / Q000379:methods*; D051379:Mice; D011993:Recombinant Fusion Proteins / Q000235:genetics* / Q000378:metabolism; D061987:Sf9 Cells; D018411:Spodoptera; D058207:Sumoylation; D044764:Ubiquitin-Activating Enzymes / Q000235:genetics* / Q000378:metabolism
chemical_list: D011993:Recombinant Fusion Proteins; D044764:Ubiquitin-Activating Enzymes