title: Hematoxylin staining reveals a decrease in nucleolar diameter of pig oocytes before germinal vesicle breakdown.
abstract: During oocyte growth, the morphology of the nucleolus changes into a compact and homogenous structure. The compact nucleoli in full-grown oocytes are not stained by aceto-orcein staining or immunofluorescence staining. In this study, we developed a hematoxylin staining method for pig oocytes in whole-mount preparations to visualize the nucleoli. Nucleoli of growing and full-grown oocytes were stained blue with hematoxylin. Using this staining method, the changes in the oocyte nucleolus during maturation were examined. The nucleolar diameter gradually decreased in maturing oocytes (10.7 ± 0.1 μm to 9.0 ± 0.7 μm, P<0.05) before germinal vesicle breakdown (GVBD). The results suggest that the nucleolar volume of oocytes decreases before GVBD.
authors.affiliation: Graduate School of Agricultural Science, Kobe University, Kobe 657-8501, Japan.
authors.forename: Tadatoshi
authors.initials: T
authors.lastname: Nobata
authors.forename: Hirohisa
authors.initials: H
authors.lastname: Kyougoku
authors.forename: Takashi
authors.initials: T
authors.lastname: Miyano
mesh_terms: D000003:Abattoirs; D000818:Animals; D002466:Cell Nucleolus / Q000737:chemistry; D002478:Cells, Cultured; D004396:Coloring Agents / Q000737:chemistry; D054885:Cumulus Cells / Q000166:cytology / Q000502:physiology; D005260:Female; D006416:Hematoxylin / Q000737:chemistry; D059471:In Vitro Oocyte Maturation Techniques; D042282:Microdissection; D008857:Microscopy, Interference; D009865:Oocytes / Q000737:chemistry / Q000166:cytology*; D009866:Oogenesis; D053141:Organelle Size; D010053:Ovary / Q000166:cytology / Q000254:growth & development; D013194:Staining and Labeling; D034421:Sus scrofa
chemical_list: D004396:Coloring Agents; D006416:Hematoxylin