title: Small angle neutron scattering for the study of solubilised membrane proteins.
abstract: Small angle neutron scattering (SANS) is a powerful technique for investigating association states and conformational changes of biological macromolecules in solution. SANS is of particular interest for the study of the multi-component systems, as membrane protein complexes, for which in vitro characterisation and structure determination are often difficult. This article details the important physical properties of surfactants in view of small angle neutron scattering studies and the interest to deuterate membrane proteins for contrast variation studies. We present strategies for the production of deuterated membrane proteins and methods for quality control. We then review some studies on membrane proteins, and focus on the strategies to overcome the intrinsic difficulty to eliminate homogeneously the detergent or surfactant signal for solubilised membrane proteins, or that of lipids for membrane proteins inserted in liposomes.
authors.affiliation: Univ. Grenoble Alpes, Institut de Biologie Structurale (IBS), F-38027, Grenoble, France.
authors.forename: Cécile
authors.initials: C
authors.lastname: Breyton
authors.forename: Frank
authors.initials: F
authors.lastname: Gabel
authors.forename: Mathilde
authors.initials: M
authors.lastname: Lethier
authors.forename: Ali
authors.initials: A
authors.lastname: Flayhan
authors.forename: Grégory
authors.initials: G
authors.lastname: Durand
authors.forename: Jean-Michel
authors.initials: JM
authors.lastname: Jault
authors.forename: Céline
authors.initials: C
authors.lastname: Juillan-Binard
authors.forename: Lionel
authors.initials: L
authors.lastname: Imbert
authors.forename: Martine
authors.initials: M
authors.lastname: Moulin
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Ravaud
authors.forename: Michael
authors.initials: M
authors.lastname: Härtlein
authors.forename: Christine
authors.initials: C
authors.lastname: Ebel
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000737:chemistry / Q000302:isolation & purification; D061249:Batch Cell Culture Techniques / Q000295:instrumentation / Q000379:methods; D003903:Deuterium / Q000737:chemistry; D006801:Humans; D008081:Liposomes / Q000737:chemistry; D008565:Membrane Proteins / Q000737:chemistry* / Q000302:isolation & purification; D033363:Neutron Diffraction / Q000379:methods*; D053838:Scattering, Small Angle; D013501:Surface-Active Agents / Q000737:chemistry
chemical_list: D001426:Bacterial Proteins; D008081:Liposomes; D008565:Membrane Proteins; D013501:Surface-Active Agents; D003903:Deuterium