title: Genome-wide expression analysis of HSP70 family genes in rice and identification of a cytosolic HSP70 gene highly induced under heat stress.
abstract: The heat shock protein 70 (HSP70) gene family plays a key role in protecting plant cells or tissues from thermal or oxidative stress. Although many studies have elucidated the molecular functions of individual family members, genome-wide analysis of this family is still limited, especially for crop species. Our objective was to integrate various meta-profiling data into the context of a phylogenetic tree, which would enable us to perform fine evaluation of functional dominancy or redundancy within this family. Our data indicated that a loss-of-function mutant of a rice cytosolic HSP70 gene (OsctHSP70-1) did not show a clear defective phenotype in response to high temperature because of the existence of another gene family member that was closely clustered with OsctHSP70-1 and had similar expression patterns. Moreover, the second gene showed much stronger anatomical expression. We indirectly analyzed the function of OsctHSP70-1 by studying GUS activity under the control of the endogenous promoter. We also designed a probable interaction network mediated by OsctHSP70-1 and used co-expression analysis among its components to refine the network, suggesting more probable model to explain the function of OsctHSP70-1.
authors.affiliation: Department of Plant Molecular Systems Biotechnology and Crop Biotech Institute, Kyung Hee University, Yongin 446-701, South Korea. khjung2010@khu.ac.kr
authors.forename: Ki-Hong
authors.initials: KH
authors.lastname: Jung
authors.forename: Hyun-Jung
authors.initials: HJ
authors.lastname: Gho
authors.forename: Minh Xuan
authors.initials: MX
authors.lastname: Nguyen
authors.forename: Sung-Ryul
authors.initials: SR
authors.lastname: Kim
authors.forename: Gynheung
authors.initials: G
authors.lastname: An
mesh_terms: D017360:Arabidopsis / Q000235:genetics* / Q000254:growth & development; D003600:Cytosol / Q000378:metabolism; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D018745:Genome, Plant; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics* / Q000502:physiology; D018869:Heat-Shock Response / Q000235:genetics*; D006358:Hot Temperature; D009154:Mutation; D020411:Oligonucleotide Array Sequence Analysis; D012275:Oryza / Q000235:genetics* / Q000502:physiology; D010802:Phylogeny; D017386:Sequence Homology, Amino Acid
chemical_list: D018840:HSP70 Heat-Shock Proteins