title: Involvement of microRNAs in infection of silkworm with bombyx mori cytoplasmic polyhedrosis virus (BmCPV).
abstract: Bombyx mori cytoplasmic polyhedrosis virus (BmCPV) is one of the most important pathogens of silkworm. MicroRNAs (miRNAs) have been demonstrated to play key roles in regulating host-pathogen interaction. However, there are limited reports on the miRNAs expression profiles during insect pathogen challenges. In this study, four small RNA libraries from BmCPV-infected midgut of silkworm at 72 h post-inoculation and 96 h post-inoculation and their corresponding control midguts were constructed and deep sequenced. A total of 316 known miRNAs (including miRNA*) and 90 novel miRNAs were identified. Fifty-eight miRNAs displayed significant differential expression between the infected and normal midgut (P value < = 0.01 and fold change > = 2.0 or < = 0.5), among which ten differentially expressed miRNA were validated by qRT-PCR method. Further bioinformatics analysis of predicted target genes of differentially expressed miRNAs showed that the miRNA targets were involved in stimulus and immune system process in silkworm.
authors.affiliation: Sericultural Research Institute, Jiangsu University of Science and Technology, Zhenjiang Jiangsu, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Wu
authors.forename: Shaohua
authors.initials: S
authors.lastname: Han
authors.forename: Tao
authors.initials: T
authors.lastname: Chen
authors.forename: Guangxing
authors.initials: G
authors.lastname: Qin
authors.forename: Long
authors.initials: L
authors.lastname: Li
authors.forename: Xijie
authors.initials: X
authors.lastname: Guo
mesh_terms: D000818:Animals; D012831:Bombyx / Q000235:genetics* / Q000821:virology*; D004064:Digestive System / Q000378:metabolism / Q000821:virology; D020869:Gene Expression Profiling; D015723:Gene Library; D063990:Gene Ontology; D054884:Host-Pathogen Interactions; D035683:MicroRNAs / Q000235:genetics*; D012087:Reoviridae / Q000502:physiology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors
chemical_list: D035683:MicroRNAs