title: Retrieval and in vitro maturation of human oocytes from ovaries removed during surgery for endometrial carcinoma: a novel strategy for human oocyte research.
abstract: OBJECTIVE
  To collect human oocytes from ovaries removed as part of surgical treatment for endometrial carcinoma, and to induce in vitro maturation of such oocytes to obtain material for research on human ovarian aging.
  
  
  METHODS
  
  
  
  METHODS
  Prospective clinical study.
  
  
  METHODS
  University Hospital.
  
  
  METHODS
  Eight patients aged 35-44 years with a preoperative diagnosis of Stage I endometrial cancer agreed to participate in this project.
  
  
  METHODS
  Surgically removed ovaries were punctured; oocytes were collected from follicular fluid and matured in vitro. Immunofluorescent detection of microtubules and DNA labeling were performed after in vitro maturation.
  
  
  METHODS
  Number of oocytes collected and their in vitro maturation stage.
  
  
  RESULTS
  In total, 87 oocytes were collected, 11 of which had completed metaphase II. Of the oocytes collected, 75 % were from three patients in their 30s, while the remaining 25 % were from five patients in their 40s. Several stages of oocytes were collected and the detection of microtubule arrangement and chromatin in various stages using fluorescence was possible.
  
  
  CONCLUSIONS
  Material for research on human ovarian aging can be obtained from ovaries removed during surgery for endometrial cancer.
authors.affiliation: Department of Obstetrics and Gynecology, Akita University Graduate School of Medicine, 1-1-1, Hondo, Akita, 010-8543, Japan.
authors.forename: Hiromitsu
authors.initials: H
authors.lastname: Shirasawa
authors.forename: Jin
authors.initials: J
authors.lastname: Kumagai
authors.forename: Wataru
authors.initials: W
authors.lastname: Sato
authors.forename: Yukiyo
authors.initials: Y
authors.lastname: Kumazawa
authors.forename: Naoki
authors.initials: N
authors.lastname: Sato
authors.forename: Yukihiro
authors.initials: Y
authors.lastname: Terada
mesh_terms: D000328:Adult; D016889:Endometrial Neoplasms / Q000473:pathology* / Q000601:surgery; D005260:Female; D005307:Fertilization in Vitro; D006801:Humans; D059471:In Vitro Oocyte Maturation Techniques; D008540:Meiosis / Q000235:genetics; D009865:Oocytes / Q000254:growth & development*; D006080:Ovarian Follicle / Q000254:growth & development* / Q000601:surgery; D010052:Ovariectomy; D011446:Prospective Studies