title: DNA binding and bending by Sac7d is stepwise.
abstract: Which came first? Using thermodynamic cycles constructed from experimental measures of the binding free energy and free energy simulations, we have shown that binding of Sulfolobus acidocaldarius protein Sac7d to DNA occurs before DNA bending, thus indicating that a conformational selection mechanism is unlikely to be operative in Sac7d binding.
authors.affiliation: Department of Chemistry, University of South Florida, 4202 E. Fowler Ave. CHE 205, Tampa, FL 33620, USA.
authors.forename: Justin
authors.initials: J
authors.lastname: Spiriti
authors.forename: Arjan
authors.initials: A
authors.lastname: van der Vaart
mesh_terms: D019843:Archaeal Proteins / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D004268:DNA-Binding Proteins / Q000737:chemistry*; D008968:Molecular Conformation; D011485:Protein Binding; D013816:Thermodynamics
chemical_list: D019843:Archaeal Proteins; D004268:DNA-Binding Proteins; C095116:Sac7 protein, Sulfolobus; D004247:DNA
keywords: DNA; DNA recognition; conformational selection; intercalation; molecular dynamics