title: Comparative analysis of mesenchymal stem cell surface marker expression for human dental mesenchymal stem cells.
abstract: OBJECTIVE
  Human dental mesenchymal stem cells (hDMSCs) have been isolated from extracted human teeth and proven to have different proliferation and differentiation abilities among the subtypes. Despite increasing interest in the clinical use of hDMSCs, a well-defined specific marker has been absent for these stem cells. In this study, a comparative analysis with known mesenchymal stem cell surface markers such as STRO-1, CD90, CD146, CD34 and TfR (CD71) was performed.
  
  
  METHODS
  Four subtypes of the hDMSCs were obtained and cultured. The hDMSCs were processed by flow cytometric analysis, fluorescence immunocytostaining for in vitro study and in situ immunohistochemical staining for in vivo study.
  
  
  CONCLUSIONS
  The previously known positive and negative MSC markers, such as STRO-1, CD90, CD146 and CD34 showed comparative expression profiles of hDMSC subtypes. TfR was highly positive in hDMSCs compared with the control cells; therefore, TfR was suggested as a new marker for hDMSCs in this study.
authors.affiliation: Experimental Immunology Branch, National Cancer Institute, NIH, Bethesda, MD 20892, USA.
authors.forename: Joo-Young
authors.initials: JY
authors.lastname: Park
authors.forename: Ho-Jin
authors.initials: HJ
authors.lastname: Jeon
authors.forename: Tae Yun
authors.initials: TY
authors.lastname: Kim
authors.forename: Kyeong-Yeoll
authors.initials: KY
authors.lastname: Lee
authors.forename: Kyoungsook
authors.initials: K
authors.lastname: Park
authors.forename: Eui Seok
authors.initials: ES
authors.lastname: Lee
authors.forename: Je-Min
authors.initials: JM
authors.lastname: Choi
authors.forename: Chung-Gyu
authors.initials: CG
authors.lastname: Park
authors.forename: Soung-Hoo
authors.initials: SH
authors.lastname: Jeon
mesh_terms: D050156:Adipogenesis; D000293:Adolescent; D000328:Adult; D000818:Animals; D015415:Biomarkers / Q000378:metabolism*; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D004622:Embryo, Mammalian / Q000166:cytology / Q000378:metabolism; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism*; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D051379:Mice; D008813:Mice, Inbred ICR; D041681:NIH 3T3 Cells; D010012:Osteogenesis; D014070:Tooth / Q000166:cytology* / Q000378:metabolism; D055815:Young Adult
chemical_list: D015415:Biomarkers; D008565:Membrane Proteins