title: Preparative isolation and purification of five steroid saponins from Dioscorea zingiberensis C.H.Wright by counter-current chromatography coupled with evaporative light scattering detector.
abstract: A counter-current chromatography (CCC) method was successfully applied to separate and purify steroid saponins from the traditional Chinese medicine Dioscorea zingiberensis C.H.Wright for the first time. Ethyl acetate-n-butanol-methanol-water (4:1:2:4, v/v) was used as the two-phase solvent system, and evaporative light scattering detector (ELSD) was used as the detector in this method. The method separated in a single run the following five steroid saponins: 26-O-β-d-glucopyranosyl-(25R)-furost-5-en-3β, 22ζ, 26-triol-3-O-[β-d-glucopyranosyl-(1→3)-β-d-glucopyranol-(1→4)-α-l-rhamnopyranosyl-(1→2)]-β-d-glucopyranoside (Compound A); 26-O-β-d-glucopyranosyl-(25R)-furost-5-en-3β, 22ζ, 26-triol-3-O-[β-d-glucopyranosyl(1→3)-α-l-rhamnopyranosyl(1→2)]-β-d-glucopyranoside (Compound B); 26-O-β-d-glucopyranosyl-(25R)-furost-5-en-3β, 22ζ, 26-triol-3-O-[α-l-rhamnopyranosyl(1→4)]-β-d-glucopyranoside (Compound C); 26-O-β-d-glucopyranosyl-(25R)-furost-5, 20(22)-diene-3β, 26-diol-3-O-{α-l-rhamnopyranosyl-(1→4)-[β-d-glucopyranosyl-(1→3)-β-d-glucopyranosyl-(1→2)]}-β-d-glucopyranoside (Compound D); and 26-O-β-d-glucopyranosyl-(25R)-furost-5, 20(22)-diene-3β, 26-diol-3-O-[β-d-glucopyranosyl-(1→4)-α-l-rhamnopyranosyl(1→2)]-β-d-glucopyranoside (Compound E). Their structural identification of the five steroid saponins was performed by means of ESI-MS, and (13)C NMR.
authors.affiliation: Biomedicine Key Laboratory of Shaanxi Province, Northwest University, Xi'an 710069, China.
authors.forename: Xinxin
authors.initials: X
authors.lastname: Zhang
authors.forename: Yoichiro
authors.initials: Y
authors.lastname: Ito
authors.forename: Jinru
authors.initials: J
authors.lastname: Liang
authors.forename: Qi
authors.initials: Q
authors.lastname: Su
authors.forename: Yongmin
authors.initials: Y
authors.lastname: Zhang
authors.forename: Jianli
authors.initials: J
authors.lastname: Liu
authors.forename: Wenji
authors.initials: W
authors.lastname: Sun
mesh_terms: D020001:1-Butanol / Q000737:chemistry; D000085:Acetates / Q000737:chemistry; D002845:Chromatography / Q000379:methods*; D027742:Dioscorea / Q000737:chemistry*; D008516:Medicine, Chinese Traditional / Q000379:methods; D000432:Methanol / Q000737:chemistry; D012503:Saponins / Q000737:chemistry* / Q000302:isolation & purification*; D013256:Steroids / Q000737:chemistry* / Q000302:isolation & purification*; D014867:Water / Q000737:chemistry
chemical_list: D000085:Acetates; D012503:Saponins; D013256:Steroids; D014867:Water; C007650:ethyl acetate; D020001:1-Butanol; D000432:Methanol
keywords: Counter-current chromatography; Dioscorea zingiberensis C.H. Wright; Evaporative light scattering detector; Steroid saponins; Traditional Chinese medicine