title: Transfection of bone marrow mesenchymal stem cells using green fluorescence protein labeled hVEGF165 recombinant plasmid mediated by liposome.
abstract: OBJECTIVE
  To study the role of bone marrow mesenchymal stem cells (BMSCs) in construction of vascularized engineered tissue.
  
  
  METHODS
  hVEGF165 was amplified via RT-PCR before recombinant with pShuttle- green fluorescence protein;green fluorescent protein (GFP)-CMV. Then the recombinant shuttle plasmid was transfected into BMSCs with Lipofectamine(TM) 2000 for packaging and amplifying. hVEGF165 mRNA expression in BMSCs cells was tested.
  
  
  RESULTS
  The sequence of hVEGF165 in pShuttle-GFP-hVEGF165 plasmid was confirmed by double-enzyme cleavage method and sequencing. hVEGF165 was highly expressed in BMSCs.
  
  
  CONCLUSIONS
  The GFP/hVEGF165 recombinant plasmid vector was constructed successfully and expressed effectively in host cells, which may be helpful for discussing the possibility of the application of VEGF165-BMSCs in tissue engineering and ischemic disease cure.
authors.affiliation: Dental Medical Center, People's Hospital of Hainan Province, Haikou 570311, Hainan, China. 1293379391@qq.com
authors.forename: Tao
authors.initials: T
authors.lastname: Wang
authors.forename: Tian-An
authors.initials: TA
authors.lastname: Liao
authors.forename: Shao-Bo
authors.initials: SB
authors.lastname: Zhong
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000378:metabolism*; D002460:Cell Line; D049452:Green Fluorescent Proteins / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D008081:Liposomes; D059630:Mesenchymal Stem Cells / Q000378:metabolism*; D010957:Plasmids / Q000235:genetics; D051381:Rats; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D014162:Transfection; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics* / Q000378:metabolism
chemical_list: D008081:Liposomes; D011993:Recombinant Fusion Proteins; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A; D049452:Green Fluorescent Proteins
keywords: Bone marrow mesenchymal stem cells; Green fluorescent protein; Plasmid; Vascular endothelial growth factor