title: Age-related reduction of structural complexity in spleen hematopoietic tissue architecture in mice.
abstract: The effects of aging on structural complexity in hematopoietic tissue are unknown. In this work, in a mouse experimental model, we report the age-related reduction of spleen hematopoietic tissue (SHT) complexity. Spleen tissue was obtained from the total of 64 male Swiss albino mice divided into 8 age groups: newborns (0 days old), 10 days, 20 days, 30 days, 120 days, 210 days, 300 and 390 days old. SHT was stained using conventional hematoxylin/eosin, and DNA-binding toluidine blue dyes. Fractal dimension as an indicator of cellular complexity, and lacunarity as indicator of tissue heterogeneity were determined based on the binarized SHT micrographs. Results indicate that fractal dimension of mice spleen hematopoietic tissue decreases with age, while lacunarity increases. These changes/trends have been detected in SHT stained both with toluidine blue and conventional hematoxylin/eosin. Fractal dimension was negatively correlated with lacunarity. The detected reduction in complexity suggests that age-related structural changes are present in mouse SHT both in general tissue architecture and progenitor cell DNA.
authors.affiliation: Laboratory for Cellular Physiology, Institute of Medical Physiology, School of Medicine, University of Belgrade, Visegradska 26/II, 11129 Belgrade, Serbia. igor.pantic@mfub.bg.ac.rs
authors.forename: Igor
authors.initials: I
authors.lastname: Pantic
authors.forename: Jovana
authors.initials: J
authors.lastname: Paunovic
authors.forename: Gordana
authors.initials: G
authors.lastname: Basta-Jovanovic
authors.forename: Milan
authors.initials: M
authors.lastname: Perovic
authors.forename: Senka
authors.initials: S
authors.lastname: Pantic
authors.forename: Nebojsa T
authors.initials: NT
authors.lastname: Milosevic
mesh_terms: D000375:Aging / Q000473:pathology*; D000818:Animals; D004396:Coloring Agents; D017709:Fractals; D006412:Hematopoietic Stem Cells / Q000473:pathology*; D008297:Male; D051379:Mice; D013154:Spleen / Q000473:pathology*; D014048:Tolonium Chloride
chemical_list: D004396:Coloring Agents; D014048:Tolonium Chloride
keywords: Chromatin; Fractal; Intrinsic; Precursor; Stem cell