title: Conjugated polyelectrolytes with aggregation-enhanced emission characteristics: synthesis and their biological applications.
abstract: Conjugated polyelectrolytes are promising candidates for the construction of fluorescent bioprobes. In this study, a series of water-soluble fluorescent polyelectrolytes have been designed and synthesized by means of the quaternization of their tetraphenylethene-containing polyyne precursors. The polyynes can be facilely prepared through Hay-Glaser polycoupling in high yields (up to 99%) with high molecular weights (up to 38,900). All the polymers exhibit a phenomenon of aggregation-induced or -enhanced emission. The fluorimetric titrations of biomolecules such as heparin, calf thymus DNA, RNA, bovine serum albumin, and human serum albumin to buffer solutions of the polyelectrolytes suggest that they are promising fluorescent bioprobes with high sensitivity and fast response. The emission intensity of the polyelectrolytes is enhanced by up to sevenfold upon binding with biomolecules through electrostatic and hydrophobic cooperative interactions. The polyelectrolytes can also serve as fluorescent visualizers for intracellular imaging with good biocompatibility and low autofluorescence interference.
authors.affiliation: The Hong Kong University of Science & Technology (HKUST), Shenzhen Research Institute, No. 9 Yuexing 1st RD, South Area, Hi-tech Park, Nanshan, Shenzhen, 518057 (China), Fax: (+85) 2-23581594; Department of Chemistry, Institute of Molecular Functional Materials and Division of Biomedical Engineering, HKUST, Clear Water Bay, Kowloon, Hong Kong (P.R. China).
authors.forename: Rongrong
authors.initials: R
authors.lastname: Hu
authors.forename: Ruquan
authors.initials: R
authors.lastname: Ye
authors.forename: Jacky W Y
authors.initials: JW
authors.lastname: Lam
authors.forename: Min
authors.initials: M
authors.lastname: Li
authors.forename: Chris W T
authors.initials: CW
authors.lastname: Leung
authors.forename: Ben Zhong
authors.initials: BZ
authors.lastname: Tang
mesh_terms: D000818:Animals; D002417:Cattle; D004247:DNA / Q000032:analysis; D004573:Electrolytes / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000138:chemical synthesis* / Q000737:chemistry; D005470:Fluorometry; D006493:Heparin / Q000032:analysis; D006801:Humans; D011108:Polymers / Q000737:chemistry*; D053279:Polyynes / Q000737:chemistry; D012313:RNA / Q000032:analysis; D012709:Serum Albumin / Q000032:analysis; D012710:Serum Albumin, Bovine / Q000032:analysis
chemical_list: D004573:Electrolytes; D005456:Fluorescent Dyes; D011108:Polymers; D012709:Serum Albumin; D053279:Polyynes; D012710:Serum Albumin, Bovine; D012313:RNA; D006493:Heparin; D004247:DNA; C427203:calf thymus DNA
keywords: aggregation; electrolytes; fluorescent probes; imaging agents; polymers