title: Genetic modifier screens to identify components of a redox-regulated cell adhesion and migration pathway.
abstract: Under normal physiological conditions, cells use oxidants, particularly H2O2, for signal transduction during processes such as proliferation and migration. Though recent progress has been made in determining the precise role H2O2 plays in these processes, many gaps still remain. To further understand this, we describe the use of a dominant enhancer screen to identify novel components of a redox-regulated cell migration and adhesion pathway in Drosophila melanogaster. Here, we discuss our methodology and progress as well as the benefits and limitations of applying such an approach to study redox-regulated pathways. Depending on the nature of these pathways, unbiased genetic modifier screens may prove a productive way to identify novel redox-regulated signaling components.
authors.affiliation: Department of Cell Biology, HHMI and Kimmel Center for Biology and Medicine of the Skirball Institute, New York University School of Medicine, New York, USA. thomas.hurd@med.nyu.edu
authors.forename: Thomas Ryan
authors.initials: TR
authors.lastname: Hurd
authors.forename: Michelle Gail
authors.initials: MG
authors.lastname: Leblanc
authors.forename: Leonard Nathaniel
authors.initials: LN
authors.lastname: Jones
authors.forename: Matthew
authors.initials: M
authors.lastname: DeGennaro
authors.forename: Ruth
authors.initials: R
authors.lastname: Lehmann
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D002465:Cell Movement; D003433:Crosses, Genetic; D029721:Drosophila Proteins / Q000235:genetics* / Q000378:metabolism; D004331:Drosophila melanogaster / Q000196:embryology / Q000235:genetics*; D004625:Embryo, Nonmammalian; D018507:Gene Expression Regulation, Developmental; D060045:Genes, Modifier; D005854:Germ Cells / Q000166:cytology / Q000378:metabolism*; D009154:Mutation; D010084:Oxidation-Reduction; D010544:Peroxidases / Q000235:genetics* / Q000378:metabolism; D015398:Signal Transduction
chemical_list: D029721:Drosophila Proteins; C404709:Jafrac1 protein, Drosophila; D010544:Peroxidases
keywords: Adhesion; Drosophila; Embryo; Hydrogen peroxide; Migration; Peroxiredoxin