title: Unusual tubulin-clustering ability of specifically c7-modified colchicine analogues.
abstract: Highly cytotoxic C7-modified colchicine analogues, exemplified by tubuloclustin, promote microtubule disassembly followed by the formation of very stable tubulin clusters, both in vitro and in cells. The proposed mechanism of action of tubuloclustin and its analogues, beyond that of colchicine, includes additional specific interactions with the α-tubulin subunit.
authors.affiliation: M. V. Lomonosov Moscow State University, Leninskie Gory, 119992 Moscow, Russian Federation.
authors.forename: Olga N
authors.initials: ON
authors.lastname: Zefirova
authors.forename: Heiko
authors.initials: H
authors.lastname: Lemcke
authors.forename: Margareta
authors.initials: M
authors.lastname: Lantow
authors.forename: Evgeniya V
authors.initials: EV
authors.lastname: Nurieva
authors.forename: Birgit
authors.initials: B
authors.lastname: Wobith
authors.forename: Galina E
authors.initials: GE
authors.lastname: Onishchenko
authors.forename: Antje
authors.initials: A
authors.lastname: Hoenen
authors.forename: Gareth
authors.initials: G
authors.lastname: Griffiths
authors.forename: Nikolay S
authors.initials: NS
authors.lastname: Zefirov
authors.forename: Sergei A
authors.initials: SA
authors.lastname: Kuznetsov
mesh_terms: D000218:Adamantane / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology; D000818:Animals; D002478:Cells, Cultured; D003078:Colchicine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology*; D003603:Cytotoxins / Q000737:chemistry / Q000494:pharmacology; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D006367:HeLa Cells; D006801:Humans; D051379:Mice; D046529:Microscopy, Electron, Transmission; D015394:Molecular Structure; D011485:Protein Binding / Q000187:drug effects; D014404:Tubulin / Q000378:metabolism*
chemical_list: D003603:Cytotoxins; C586671:N-(7-adamant-2-yloxy-7-oxoheptanoyl)-N-deacetylcolchicine; D014404:Tubulin; D000218:Adamantane; D003078:Colchicine
keywords: anticancer agents; apoptosis; colchicine; cytotoxicity; microtubules; tubulin