title: Coronin 1 is dispensable for leukocyte recruitment and liver injury in concanavalin A-induced hepatitis.
abstract: Coronin 1, a member of the evolutionary conserved coronin protein family, is highly expressed in all leukocytes. In mice and human, genetic inactivation of coronin 1 results in immuno-deficiencies that are linked to a strong reduction of naïve T cell numbers in peripheral organs, while memory/effector T cells, B cells, monocytes and neutrophils are less or not at all affected. Whether or not coronin 1 is important for leukocyte functions such as migration and phagocytosis has been a matter of debate. The current work addresses coronin 1-dependent leukocyte function by analyzing the response of coronin 1-deficient mice in a model of concanavalin A (Con A)-induced liver injury. Histological evaluation and determination of serum liver enzyme levels showed that coronin 1-deficient mice develop signs of acute hepatitis similar to Con A-treated wild type mice despite a reduced activation of T cells in the absence of coronin 1. Furthermore, analysis by intravital microscopy following Con A stimulation revealed that Gr-1+ neutrophils and CD4+ T cell adhesion in the post-sinusoidal venules increased in wild type as well as in coronin 1-deficient mice. These results suggest that coronin 1, while important for naïve T cell survival, is dispensable for other leukocyte function under inflammatory conditions in vivo.
authors.affiliation: Biozentrum, University of Basel, Klingelbergstrasse 50, CH-4056 Basel, Switzerland. Kerstin.siegmund@i-med.ac.at
authors.forename: Kerstin
authors.initials: K
authors.lastname: Siegmund
authors.forename: Woo-Yong
authors.initials: WY
authors.lastname: Lee
authors.forename: Vincent S
authors.initials: VS
authors.lastname: Tchang
authors.forename: Michael
authors.initials: M
authors.lastname: Stiess
authors.forename: Luigi
authors.initials: L
authors.lastname: Terracciano
authors.forename: Paul
authors.initials: P
authors.lastname: Kubes
authors.forename: Jean
authors.initials: J
authors.lastname: Pieters
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D002448:Cell Adhesion / Q000276:immunology; D002465:Cell Movement / Q000276:immunology; D002470:Cell Survival / Q000276:immunology; D056486:Chemical and Drug Induced Liver Injury; D003208:Concanavalin A; D006505:Hepatitis / Q000378:metabolism*; D007249:Inflammation / Q000276:immunology; D007962:Leukocytes / Q000276:immunology*; D008099:Liver / Q000276:immunology* / Q000293:injuries; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008840:Microfilament Proteins / Q000235:genetics / Q000378:metabolism*; D009504:Neutrophils / Q000276:immunology
chemical_list: D008840:Microfilament Proteins; D003208:Concanavalin A; C072061:coronin proteins
keywords: Concanavalin A; Coronin 1; Hepatitis; Leukocytes