title: Free radical scavenging potential of in vitro raised and greenhouse acclimatized plants of Artemisia amygdalina.
abstract: OBJECTIVE
  Artemisia amygdalina Decne. (Asteraceae) is a critically endangered and endemic herb of Kashmir Himalayan sub-alpine region and Pakistan. Scientific research throughout the world has evidence to support the tremendous medicinal utility of the genus Artemisia. The natural resources of medicinal plants are being reduced day by day. This study provides the alternative way for medicinal resource utilization and conservation of A. amygdalina.
  
  
  METHODS
  In vitro-raised plants and greenhouse acclimatized plants were obtained by culturing wild explants on Murashige and Skoog's medium. Plant extracts were obtained and subjected to different antioxidant assays: DPPH assay, riboflavin photo-oxidation assay, deoxy ribose assay, ferric thiocyanate assay, thiobarbituric acid assay, post mitochondrial supernatant assay and DNA damage on agarose gel.
  
  
  RESULTS
  In vitro grown plants, as well as those acclimatized in the greenhouse reveals antioxidant activity against hydroxyl, superoxide, and lipid peroxyl radicals.
  
  
  CONCLUSIONS
  This preliminary study revealed the free radical scavenging potential of tissue culture-raised plant extracts of A. amydalina.
authors.affiliation: Department of Biochemistry, University of Kashmir, Hazratbal, Srinagar 190006, Jammu and Kashmir, India. rasoolrafiya@gmail.com
authors.forename: R
authors.initials: R
authors.lastname: Rasool
authors.forename: B A
authors.initials: BA
authors.lastname: Ganai
authors.forename: S
authors.initials: S
authors.lastname: Akbar
authors.forename: A N
authors.initials: AN
authors.lastname: Kamili
mesh_terms: D000064:Acclimatization; D019306:Artemisia / Q000737:chemistry* / Q000254:growth & development / Q000502:physiology; D016166:Free Radical Scavengers / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry*; D046509:Tissue Culture Techniques
chemical_list: D016166:Free Radical Scavengers; D010936:Plant Extracts
keywords: Antioxidant; Artemisia amygdalina; DPPH; Free radicals; Tissue culture