title: Cis-9-octadecenoic acid from the rhizospheric bacterium Stenotrophomonas maltophilia BJ01 shows quorum quenching and anti-biofilm activities.
abstract: Quorum quenching (QQ) is an effective approach for the prevention of bacterial infections involving biofilms. This study reports the QQ and anti-biofilm activities of a rhizospheric bacterium identified as Stenotrophomonas maltophilia BJ01. The QQ activity was demonstrated using Chromobacterium violaceum CV026 as a biosensor. A maximum of 95% reduction in violacein production, a quorum sensing-regulated behavior, was observed. Gas chromatography-mass spectroscopy of the extract showed that the active compound was cis-9-octadecenoic acid, which was confirmed by electronspray ionization-mass spectroscopy data. The extract also inhibited biofilm formation of Pseudomonas aeruginosa ATCC 9027 without affecting its growth. Scanning electron and atomic force microscopy showed architectural disruption of the biofilm when treated with the extract. This is the first report of the QQ and anti-biofilm activities of cis-9-octadecenoic acid isolated from any bacterium. It may have the potential to combat detrimental infections with P. aeruginosa. Further validation is required for any possible medical application.
authors.affiliation: Discipline of Marine Biotechnology and Ecology, CSIR-Central Salt and Marine Chemicals Research Institute, Bhavnagar, India.
authors.forename: Vijay Kumar
authors.initials: VK
authors.lastname: Singh
authors.forename: Kumari
authors.initials: K
authors.lastname: Kavita
authors.forename: Rathish
authors.initials: R
authors.lastname: Prabhakaran
authors.forename: Bhavanath
authors.initials: B
authors.lastname: Jha
mesh_terms: D018441:Biofilms / Q000187:drug effects*; D057219:Biofouling / Q000517:prevention & control*; D008401:Gas Chromatography-Mass Spectrometry; D018625:Microscopy, Atomic Force; D008855:Microscopy, Electron, Scanning; D019301:Oleic Acid / Q000737:chemistry / Q000494:pharmacology*; D011550:Pseudomonas aeruginosa / Q000502:physiology*; D053038:Quorum Sensing / Q000187:drug effects*; D021241:Spectrometry, Mass, Electrospray Ionization; D020615:Stenotrophomonas maltophilia / Q000737:chemistry*
chemical_list: D019301:Oleic Acid