title: DNA vaccine coding for the rhesus prostate specific antigen delivered by intradermal electroporation in patients with relapsed prostate cancer.
abstract: We tested safety, clinical efficacy and immunogenicity of a DNA vaccine coding for rhesus prostate specific antigen (PSA) delivered by intradermal injection and skin electroporation. Fifteen patients with biochemical relapse of prostate cancer without macroscopic disease participated in this phase I study. Patients were started on a 1 month course of androgen deprivation therapy (ADT) prior to treatment. Vaccine doses ranged from 50 to 1,600 μg. Study subjects received five vaccinations at four week intervals. All patients have had at least one year of follow-up. No systemic toxicity was observed. Discomfort from electroporation did not require analgesia or topical anesthetic. No clinically significant changes in PSA kinetics were observed as all patients required antiandrogen therapy shortly after completion of the 5 months of vaccination due to rising PSA. Immunogenicity, as measured by T-cell reactivity to the modified PSA peptide and to a mix of overlapping PSA peptides representing the full length protein, was observed in some patients. All but one patient had pre-study PSA specific T-cell reactivity. ADT alone resulted in increases in T-cell reactivity in most patients. Intradermal vaccination with skin electroporation is easily performed with only minor discomfort for the patient. Patients with biochemical relapse of prostate cancer are a good model for testing immune therapies.
authors.affiliation: Department of Immunology, Genetics & Pathology, Rudbeck Laboratory Uppsala University, 751 85 Uppsala, Sweden. fredrik.eriksson@igp.uu.se
authors.forename: Fredrik
authors.initials: F
authors.lastname: Eriksson
authors.forename: Thomas
authors.initials: T
authors.lastname: Tötterman
authors.forename: Anna-Karin
authors.initials: AK
authors.lastname: Maltais
authors.forename: Pavel
authors.initials: P
authors.lastname: Pisa
authors.forename: Jeffrey
authors.initials: J
authors.lastname: Yachnin
mesh_terms: D000368:Aged; D000818:Animals; D018274:Electroporation; D006801:Humans; D007167:Immunotherapy / Q000379:methods; D007271:Injections, Intradermal; D008253:Macaca mulatta / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D017430:Prostate-Specific Antigen / Q000235:genetics / Q000276:immunology / Q000493:pharmacokinetics / Q000627:therapeutic use*; D011471:Prostatic Neoplasms / Q000276:immunology / Q000628:therapy*; D013601:T-Lymphocytes / Q000276:immunology; D016896:Treatment Outcome; D014611:Vaccination / Q000379:methods; D019444:Vaccines, DNA / Q000008:administration & dosage*
chemical_list: D019444:Vaccines, DNA; D017430:Prostate-Specific Antigen
keywords: Cancer; Electroporation; Intradermal delivery; Prostate cancer; Vaccine