title: Vasculoprotective effects of combined endothelial progenitor cells and mesenchymal stem cells in diabetic wound care: their potential role in decreasing wound-oxidative stress.
abstract: To investigate whether the combined endothelial progenitor cells (EPCs) and mesenchymal stem cells (MSCs) could enhance angiogenesis and wound healing in diabetic mice. Balb/c nude mice were divided into five groups, including a control group, diabetic group (DM), DM injected with 1 × 10(6) cells MSCs, DM injected with 1 × 10(6) cells EPCs, and DM injected with combined 0.5 × 10(6) cells MSCs and 0.5 × 10(6) cells EPCs. After seven weeks, the mice were anesthetized, and bilateral full-thickness excision skin wounds were made on the dorsorostral back. The percentage of wound closure in DM group decreased significantly than in control and all other treated groups on day 7 and day 14 (P < 0.005). On day 14, the percentage of capillary vascularity in combine-treated group was significantly higher than in DM (P < 0.005). In the present study, we have demonstrated that the combined EPCs and MSCs can increase vascular endothelial growth factor (VEGF) level and angiogenesis which resulted in reduced neutrophil infiltration, decreased malondialdehyde (MDA) levels, and enhanced wound healing in diabetic mice model.
authors.affiliation: Inter-Department of Physiology, Graduate School, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand.
authors.forename: Supakanda
authors.initials: S
authors.lastname: Sukpat
authors.forename: Nipan
authors.initials: N
authors.lastname: Isarasena
authors.forename: Jutamas
authors.initials: J
authors.lastname: Wongphoom
authors.forename: Suthiluk
authors.initials: S
authors.lastname: Patumraj
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D001835:Body Weight / Q000187:drug effects; D002196:Capillaries / Q000187:drug effects / Q000473:pathology*; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000473:pathology; D042783:Endothelial Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D005337:Fibrin / Q000494:pharmacology; D006801:Humans; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D018919:Neovascularization, Physiologic / Q000187:drug effects; D020556:Neutrophil Infiltration / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects; D033581:Stem Cell Transplantation; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism; D014945:Wound Healing / Q000187:drug effects
chemical_list: D001786:Blood Glucose; D042461:Vascular Endothelial Growth Factor A; D008315:Malondialdehyde; D005337:Fibrin