title: Directed co-evolution of an endoglucanase and a β-glucosidase in Escherichia coli by a novel high-throughput screening method.
abstract: A novel high-throughput screening method for directed co-evolution of endoglucanase and β-glucosidase was constructed based on the synergy of the two enzymes using a bicistronic operon. To our knowledge, this is the first report of directed co-evolution of two cellulosic enzymes using an insoluble screening substrate via their synergistic reactions.
authors.affiliation: Institute of Bioengineering and State Key Laboratory of Chemical Engineering, Department of Chemical and Biological Engineering, Zhejiang University, Hangzhou, 310027, PR China.
authors.forename: Min
authors.initials: M
authors.lastname: Liu
authors.forename: Jiali
authors.initials: J
authors.lastname: Gu
authors.forename: Wenping
authors.initials: W
authors.lastname: Xie
authors.forename: Hongwei
authors.initials: H
authors.lastname: Yu
mesh_terms: D002480:Cellulase / Q000235:genetics / Q000378:metabolism*; D002482:Cellulose / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology*; D057166:High-Throughput Screening Assays; D009154:Mutation; D001617:beta-Glucosidase / Q000235:genetics / Q000378:metabolism*
chemical_list: D002482:Cellulose; D001617:beta-Glucosidase; D002480:Cellulase