title: A known expressed sequence tag, BM742401, is a potent lincRNA inhibiting cancer metastasis.
abstract: Long intergenic non-coding RNAs (lincRNAs) have historically been ignored in cancer biology. However, thousands of lincRNAs have been identified in mammals using recently developed genomic tools, including microarray and high-throughput RNA sequencing (RNA-seq). Several of the lincRNAs identified have been well characterized for their functions in carcinogenesis. Here we performed RNA-seq experiments comparing gastric cancer with normal tissues to find differentially expressed transcripts in intergenic regions. By analyzing our own RNA-seq and public microarray data, we identified 31 transcripts, including a known expressed sequence tag, BM742401. BM742401 was downregulated in cancer, and its downregulation was associated with poor survival in gastric cancer patients. Ectopic overexpression of BM742401 inhibited metastasis-related phenotypes and decreased the concentration of extracellular MMP9. These results suggest that BM742401 is a potential lincRNA marker and therapeutic target.
authors.affiliation: Medical Genomics Research Center, KRIBB, Daejeon, Republic of Korea.
authors.forename: Seong-Min
authors.initials: SM
authors.lastname: Park
authors.forename: Sung-Joon
authors.initials: SJ
authors.lastname: Park
authors.forename: Hee-Jin
authors.initials: HJ
authors.lastname: Kim
authors.forename: Oh-Hyung
authors.initials: OH
authors.lastname: Kwon
authors.forename: Tae-Wook
authors.initials: TW
authors.lastname: Kang
authors.forename: Hyun-Ahm
authors.initials: HA
authors.lastname: Sohn
authors.forename: Seon-Kyu
authors.initials: SK
authors.lastname: Kim
authors.forename: Seung
authors.initials: S
authors.lastname: Moo Noh
authors.forename: Kyu-Sang
authors.initials: KS
authors.lastname: Song
authors.forename: Se-Jin
authors.initials: SJ
authors.lastname: Jang
authors.forename: Yong
authors.initials: Y
authors.lastname: Sung Kim
authors.forename: Seon-Young
authors.initials: SY
authors.lastname: Kim
mesh_terms: D000818:Animals; D021901:DNA, Intergenic / Q000235:genetics; D020224:Expressed Sequence Tags / Q000378:metabolism*; D005110:Extracellular Space / Q000378:metabolism; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D020022:Genetic Predisposition to Disease; D006801:Humans; D008297:Male; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D015999:Multivariate Analysis; D009362:Neoplasm Metastasis; D009367:Neoplasm Staging; D010641:Phenotype; D016016:Proportional Hazards Models; D062085:RNA, Long Noncoding / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D015203:Reproducibility of Results; D013274:Stomach Neoplasms / Q000235:genetics* / Q000473:pathology*; D016019:Survival Analysis
chemical_list: D021901:DNA, Intergenic; D062085:RNA, Long Noncoding; D012333:RNA, Messenger; D020780:Matrix Metalloproteinase 9