title: DNA replication-dependent binding of CTCF plays a critical role in adenovirus genome functions.
abstract: The expression of adenovirus late genes is shown to require viral DNA replication, but its mechanism remains elusive. Here we found that knockdown of CTCF suppresses viral DNA replication as well as late, but not early, gene expression. Chromatin immunoprecipitation assays indicated that CTCF binds to viral chromatin depending on viral DNA replication. These findings depict CTCF as a critical regulator for adenovirus genome functions in late phases of infection.
authors.affiliation: Department of Infection Biology, Faculty of Medicine and Graduate School of Comprehensive Human Sciences, University of Tsukuba, Tsukuba 305-8575, Japan.
authors.forename: Tetsuro
authors.initials: T
authors.lastname: Komatsu
authors.forename: Takeshi
authors.initials: T
authors.lastname: Sekiya
authors.forename: Kyosuke
authors.initials: K
authors.lastname: Nagata
mesh_terms: D000260:Adenoviruses, Human / Q000235:genetics* / Q000378:metabolism*; D000076246:CCCTC-Binding Factor; D002460:Cell Line; D002843:Chromatin / Q000378:metabolism; D047369:Chromatin Immunoprecipitation; D004261:DNA Replication; D015967:Gene Expression Regulation, Viral; D055785:Gene Knockdown Techniques; D023061:Gene Order; D016679:Genome, Viral; D006801:Humans; D011485:Protein Binding; D012097:Repressor Proteins / Q000378:metabolism*; D014158:Transcription, Genetic; D014764:Viral Proteins / Q000235:genetics
chemical_list: D000076246:CCCTC-Binding Factor; C000619697:CTCF protein, human; D002843:Chromatin; D012097:Repressor Proteins; D014764:Viral Proteins