title: Novel cholinesterase modulators and their ability to interact with DNA.
abstract: In the present work, an interaction of four cholinesterase modulators (1-4) with calf thymus DNA was studied via spectroscopic techniques (UV-Vis, fluorescent spectroscopy and circular dichroism). From UV-Vis spectroscopic analysis, the binding constants for DNA-pyridinium oximes complexes were calculated (K=3.5×10(4) to 1.4×10(5)M(-1)). All these measurements indicated that the compounds behave as effective DNA-interacting agents. Electrophoretic techniques proved that ligand 2 inhibited topoisomerase I at a concentration 5μM.
authors.affiliation: Institute of Chemistry, Department of Biochemistry, P.J. Šafárik University, Faculty of Science, Moyzesova 11, 04001 Kosice, Slovak Republic.
authors.forename: Jana
authors.initials: J
authors.lastname: Janockova
authors.forename: Zuzana
authors.initials: Z
authors.lastname: Gulasova
authors.forename: Kamil
authors.initials: K
authors.lastname: Musilek
authors.forename: Kamil
authors.initials: K
authors.lastname: Kuca
authors.forename: Maria
authors.initials: M
authors.lastname: Kozurkova
mesh_terms: D001965:Bromides / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology*; D002802:Cholinesterases / Q000378:metabolism*; D002942:Circular Dichroism; D004247:DNA / Q000378:metabolism*; D004264:DNA Topoisomerases, Type I / Q000378:metabolism; D004587:Electrophoresis, Agar Gel; D004996:Ethidium / Q000378:metabolism; D010957:Plasmids / Q000378:metabolism; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet
chemical_list: D001965:Bromides; D004247:DNA; C427203:calf thymus DNA; D002802:Cholinesterases; D004264:DNA Topoisomerases, Type I; D004996:Ethidium
keywords: Cholinesterase modulators; DNA; Topoisomerase I