title: Lipase entrapment in PVA/Chitosan biodegradable film for reactor coatings.
abstract: This study reports the development and characterization of novel biodegradable film, based on chitosan and polyvinyl alcohol containing lipase entrapped. The films showed a thickness of 70.4 and 79 μm to PVA/Chitosan and PVA/Chitosan/Lipase, respectively. The entrapment of lipase in PVA/Chitosan film resulted in increasing of 69.4% tensile strength (TS), and 52.4% of elongation. SEM images showed the formation of a continuous film, without pores or cracks. The lipase entrapment efficiency was estimated in 92% and the films were repeatedly used for 25 hydrolytic cycles, maintaining 62% of initial activity. The PVA/Chitosan/Lipase film was used for olive oil hydrolysis of high performance. These results indicate that PVA/Chitosan/Lipase is a promising material for biotechnology applications such as triacylglycerol hydrolysis and biodiesel production.
authors.affiliation: Departamento de Bioquímica e Biologia Molecular, Laboratório de Química de Proteínas, Universidade Federal de Goiás, Cx. Postal 131, 74001-970, Goiânia, GO, Brazil.
authors.forename: Karla A
authors.initials: KA
authors.lastname: Batista
authors.forename: Flavio Marques
authors.initials: FM
authors.lastname: Lopes
authors.forename: Fabio
authors.initials: F
authors.lastname: Yamashita
authors.forename: Kátia Flávia
authors.initials: KF
authors.lastname: Fernandes
mesh_terms: D019149:Bioreactors; D048271:Chitosan / Q000737:chemistry / Q000494:pharmacology*; D020099:Coated Materials, Biocompatible / Q000494:pharmacology*; D004800:Enzymes, Immobilized / Q000378:metabolism*; D007700:Kinetics; D008049:Lipase / Q000378:metabolism*; D008353:Mannitol / Q000494:pharmacology; D008855:Microscopy, Electron, Scanning; D011142:Polyvinyl Alcohol / Q000737:chemistry / Q000494:pharmacology*; D059027:Recycling; D012995:Solubility; D013718:Tensile Strength / Q000187:drug effects; D014867:Water / Q000737:chemistry
chemical_list: D020099:Coated Materials, Biocompatible; D004800:Enzymes, Immobilized; D014867:Water; D008353:Mannitol; D011142:Polyvinyl Alcohol; D048271:Chitosan; D008049:Lipase
keywords: Bioreactor; Immobilization; Olive oil hydrolysis; Repeated use; Stability