title: UBIQUITIN-SPECIFIC PROTEASE16 interacts with a HEAVY METAL ASSOCIATED ISOPRENYLATED PLANT PROTEIN27 and modulates cadmium tolerance.
abstract: Protein ubiquitination and deubiquitination are two reversible processes catalyzed by ubiquitin ligases and deubiquitinating enzymes, respectively. In Arabidopsis, lots of substrates of ubiquitin ligases were found, whereas only a few targets of deubiquitinating enzymes were identified. Recently, we reported that a functional UBIQUITIN-SPECIFIC PROTEASE16 (UBP16) was involved in salt tolerance through positively regulating plasma membrane Na+/H+ antiport activity and at least partially modulating SERINE HYDROXYMETHYLTRANSFERASE1 (SHM 1) stability and activity. Here, we report that UBP16 interacts with HEAVY META L ASSOCIATED ISOPRENYLATED PLANT PROTEIN 27 (HI PP27), a metallochaperone containing a predicted heavy-metal-associated domain, which has been reported to play an important role in cadmium detoxification. Meanwhile, the ubp16 mutant showed more sensitive to cadmium than wild-type. Taken together, HI PP27 may be another target of UBP16 in cadmium response.
authors.forename: Jinfeng
authors.initials: J
authors.lastname: Zhao
authors.forename: Huapeng
authors.initials: H
authors.lastname: Zhou
authors.forename: Yueyong
authors.initials: Y
authors.lastname: Li
mesh_terms: D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D002104:Cadmium / Q000633:toxicity*; D012696:Glycine Hydroxymethyltransferase / Q000235:genetics / Q000378:metabolism; D019216:Metals, Heavy / Q000633:toxicity*; D011485:Protein Binding; D055049:Salt Tolerance / Q000502:physiology
chemical_list: D029681:Arabidopsis Proteins; D019216:Metals, Heavy; D002104:Cadmium; D012696:Glycine Hydroxymethyltransferase; C509630:SHM1 protein, Arabidopsis