title: Role of protein kinase G on the post-shock mesenteric lymph blockage ameliorating vascular calcium sensitivity.
abstract: OBJECTIVE
  To investigate the role of protein kinase G (PKG) in blocking post-shock mesenteric lymph (PSML) return ameliorating the calcium sensitivity in hemorrhagic shock rats.
  
  
  METHODS
  Male Wistar rats were randomly divided into sham, shock, shock+ligation (shock plus mesenteric lymph duct ligation (MLDL)), shock+drainage (shock plus PSML drainage) groups. After shock (hypotension 40 mm Hg) for three hours or corresponding times, the superior mesenteric artery (SMA) was taken out for detecting the PKG and phospho PKG (p-PKG) contents, and the vascular rings of SMA were prepared for assaying the calcium sensitivity using an isolated organ perfusion system.
  
  
  RESULTS
  The PKG and p-PKG contents of SMA in shock group were significantly increased than that of sham group, and MLDL or PSML drainage reducing the levels of PKG and p-PKG. Meanwhile, the vascular calcium sensitivity in shock group was significantly lower than that of sham group, MLDL or PSML drainage enhanced the calcium sensitivity. After incubating with PKG regulators in shock+ligation and shock+drainage groups, the PKG agonist 8Br-cGMP reduced the contractility of vascular rings to gradient calcium ions and Emax and the PKG inhibitor agonist KT5823 elevated the calcium sensitivity significantly.
  
  
  CONCLUSIONS
  Protein kinase G plays an important role in post-shock mesenteric lymph blockage improving vascular calcium sensitivity.
authors.affiliation: Institute of Microcirculation, Hebei North University, Zhangjiakou, China.
authors.forename: Zi-Gang
authors.initials: ZG
authors.lastname: Zhao
authors.forename: Yan-Ling
authors.initials: YL
authors.lastname: Wei
authors.forename: Chun-Yu
authors.initials: CY
authors.lastname: Niu
authors.forename: Yu-Ping
authors.initials: YP
authors.lastname: Zhang
authors.forename: Li-Min
authors.initials: LM
authors.lastname: Zhang
authors.forename: Li-Na
authors.initials: LN
authors.lastname: Jiang
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002118:Calcium / Q000032:analysis / Q000378:metabolism*; D017869:Cyclic GMP-Dependent Protein Kinases / Q000032:analysis / Q000502:physiology*; D004797:Enzyme-Linked Immunosorbent Assay; D008297:Male; D017538:Mesenteric Artery, Superior / Q000378:metabolism* / Q000503:physiopathology; D009119:Muscle Contraction; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D012771:Shock, Hemorrhagic / Q000378:metabolism* / Q000503:physiopathology
chemical_list: D017869:Cyclic GMP-Dependent Protein Kinases; D002118:Calcium