title: Study on hemostatic activities of the rhizome of Paris bashanensis.
abstract: CONTEXT. Paris bashanensis Wang et Tang (Liliaceae) is widely used in traditional Chinese medicine for the treatment of injuries, fractures and hemorrhage in Hubei and Sichuan Province.
  
  
  OBJECTIVE
  The n-BuOH extract of Paris bashanensis was investigated for hemostatic activity and chemical constituents in order to provide a basis for the application in folk use.
  
  
  METHODS
  The n-BuOH extract of P. bashanensis was divided into three eluents (30, 50 and 70% EtOH) by macroporous adsorptive resin D101. The bleeding time of breaking tail hemostasis and clotting time of capillary and slide method in mice were used extensively to screen the hemostasis properties after repetitive administration of these three fractions (100 mg/kg, 50 mg/kg) for 5 days (total of 5 times, once per day). The chemical compounds were analyzed by HPLC-UV.
  
  
  RESULTS
  The inhibition rates in the bleeding time of 70, 50 and 30% n-BuOH ext. were 45, 32 and 21%, respectively. Using the slide method the decreasing rate of the clotting time of 70, 50 and 30% n-BuOH ext. were 71, 65 and 32% and in the experiment of capillary method, the inhibition rates were 43, 31 and 24%, respectively. A total of 70% n-BuOH ext. showed a high content of the pennogenin-type saponins by HPLC-UV.
  
  
  CONCLUSIONS
  The 70% n-BuOH ext. of P. bashanensis was found to contain high levels of pennogenin saponins, which may lead to a higher hemostatic activity. Combined with the hemostatic test, P. bashanensis could be used as a resource of hemostatic drug.
authors.affiliation: Tianjin Key Laboratory of Chemistry and Analysis of Chinese Materia Medica, Tianjin University of Traditional Chinese Medicine, Tianjin, PR China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.forename: Juan
authors.initials: J
authors.lastname: Hao
authors.forename: Wenyuan
authors.initials: W
authors.lastname: Gao
authors.forename: Zhen
authors.initials: Z
authors.lastname: Liu
authors.forename: Shanshan
authors.initials: S
authors.lastname: Wu
authors.forename: Songsong
authors.initials: S
authors.lastname: Jing
mesh_terms: D000818:Animals; D001760:Bleeding Time; D000440:Butanols / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D004305:Dose-Response Relationship, Drug; D006487:Hemostasis / Q000187:drug effects*; D006490:Hemostatics / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D019668:Liliaceae / Q000737:chemistry; D051379:Mice; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D027343:Rhizome; D012503:Saponins / Q000302:isolation & purification / Q000494:pharmacology; D012997:Solvents / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet; D013997:Time Factors; D014914:Whole Blood Coagulation Time
chemical_list: D000440:Butanols; D006490:Hemostatics; D010936:Plant Extracts; D012503:Saponins; D012997:Solvents