title: Antioxidant, mutagenic and antimutagenic activities of an aqueous extract of Limoniastrum guyonianum gall.
abstract: An aqueous extract of Limoniastrum guyonianum gall (G extract) was tested on Salmonella typhimurium to assess its mutagenic and antimutagenic effects. This extract showed no mutagenicity when tested with S. typhimurium strain TA104 either with or without exogenous metabolic activation mixture (S9), whereas our findings revealed that the aqueous gall extract induced a mutagenic effect in S. typhimurium TA1538 when tested in the presence, as well as in the absence, of S9 activation mixture at the concentration of 500 µg/mL. Thus, the same concentration produced a mutagenic effect, when incubated with S. typhimurium TA100 in the presence of metabolic activation mixture. In contrast, our results showed a weak antimutagenic potential of the same extract against sodium azide in the presence of S. typhimurium TA100 and S. typhimurium TA1538 without metabolic activation (S9), whereas, in the presence of S. typhimurium TA104, we obtained a significant inhibition percentage (76.39%) toward 3.25 µg/plate of methylmethanesulfonate. Antimutagenicity against aflatoxin B1, 4-nitro-o-phenylene-diamine and 2-aminoanthracène was significant, with an inhibition percentage of, respectively, 70.63, 99.3 and 63.37% in the presence of, respectively, S. typhimurium TA100, S. typhimurium TA1538 and S. typhimurium TA104 strains at a concentration of 250 µg/plate after metabolic activation (S9). Antioxidant capacity of the tested extract was evaluated using the enzymatic (xanthine/xanthine oxidase assay) and the nonenzymatic (2,2-diphenyl-1-picrylhydrazyl) system. G extract exhibited high antioxidant activity.
authors.affiliation: Unité de Pharmacognosie/Biologie Moléculaire, Faculté de Pharmacie de Monastir and.
authors.forename: Mounira
authors.initials: M
authors.lastname: Krifa
authors.forename: Ines
authors.initials: I
authors.lastname: Bouhlel
authors.forename: Ines
authors.initials: I
authors.lastname: Skandrani
authors.forename: Leila
authors.initials: L
authors.lastname: Chekir-Ghedira
authors.forename: Kamel
authors.initials: K
authors.lastname: Ghedira
mesh_terms: D016604:Aflatoxin B1 / Q000037:antagonists & inhibitors; D000704:Analysis of Variance; D000873:Anthracenes; D016587:Antimutagenic Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D001713:Biphenyl Compounds; D008741:Methyl Methanesulfonate; D008861:Microsomes / Q000187:drug effects; D009153:Mutagens / Q000494:pharmacology*; D010655:Phenylenediamines / Q000037:antagonists & inhibitors; D010851:Picrates; D010936:Plant Extracts / Q000494:pharmacology*; D010941:Plant Tumors; D029606:Plumbaginaceae / Q000737:chemistry*; D012486:Salmonella typhimurium / Q000187:drug effects; D019810:Sodium Azide / Q000378:metabolism; D013045:Species Specificity; D014416:Tunisia
chemical_list: D000873:Anthracenes; D016587:Antimutagenic Agents; D000975:Antioxidants; D001713:Biphenyl Compounds; D009153:Mutagens; D010655:Phenylenediamines; D010851:Picrates; D010936:Plant Extracts; C012277:1,2-diamino-4-nitrobenzene; C006593:2-anthramine; D019810:Sodium Azide; D016604:Aflatoxin B1; D008741:Methyl Methanesulfonate; C004931:1,1-diphenyl-2-picrylhydrazyl