title: Molecular profiling of genetic variability in domesticated groundnut (Arachis hypogaea L.) based on ISJ, URP, and DAMD markers.
abstract: To detect DNA polymorphisms in the peanut, we screened 26 polymorphic primers using intron-exon splice junction (ISJ), universal rice primer (URP), and directed amplification of minisatellite region DNA (DAMD) techniques. Amplification of genomic DNA of 16 peanut accessions yielded 121 ISJ, 50 URP, and 25 DAMD fragments, of which 34, 25 and 16 were polymorphic, respectively. The range of polymorphism was 10.0-62.5%, averaging 27.7%, for ISJ; 20-80%, averaging 49.5%, for URP; and 28.6-50.0%, averaging 36.3%, for DAMD. In comparisons of multiplex ratio, average polymorphism information content, and marker index, the URP markers were relatively more efficient than ISJ and DAMD markers. Clustering results remained more or less the same with ISJ and URP markers. To the best of our knowledge, this is the first report on the study of the genetic diversity of the peanut using ISJ, URP, and DAMD markers.
authors.affiliation: Cash Crops Research Institute, Guangxi Academy of Agricultural Sciences, Nanning, 530007, Guangxi, China, xfq2002@126.com.
authors.forename: Faqian
authors.initials: F
authors.lastname: Xiong
authors.forename: Junxian
authors.initials: J
authors.lastname: Liu
authors.forename: Jing
authors.initials: J
authors.lastname: Jiang
authors.forename: Ruichun
authors.initials: R
authors.lastname: Zhong
authors.forename: Liangqiong
authors.initials: L
authors.lastname: He
authors.forename: Zhuqiang
authors.initials: Z
authors.lastname: Han
authors.forename: Zhong
authors.initials: Z
authors.lastname: Li
authors.forename: Xiumei
authors.initials: X
authors.lastname: Tang
authors.forename: Ronghua
authors.initials: R
authors.lastname: Tang
mesh_terms: D010367:Arachis / Q000235:genetics*; D016000:Cluster Analysis; D017931:DNA Primers; D018744:DNA, Plant / Q000235:genetics; D005819:Genetic Markers; D014644:Genetic Variation; D018598:Minisatellite Repeats; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA
chemical_list: D017931:DNA Primers; D018744:DNA, Plant; D005819:Genetic Markers