title: [Effects of four dental alloys on apoptosis related gene and protein expression of fibroblast L929].
abstract: OBJECTIVE
  To investigate the effects of the leaching liquids of 4 differents kinds of dental alloys (Au alloy, Ag-Pd alloy, Co-Cr alloy, Ni-Cr alloy) on apoptosis related gene and protein of fibroblast L929.
  
  
  METHODS
  The L929 cells of mouse were treated in vitro with leaching liquids of 4 different kinds of dental alloys, Au alloy (group A), Ag-Pd alloy(group B), Co-Cr alloy(group C) and Ni-Cr alloy(group D). The RPMI1640 cell medium containing 10% fetal calf serum was served as a control(group E). The effects of these alloys on the expression of caspase-3, 8, 9 of L929 cells were examined by reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemistry method. Results After 48 h culture, the mRNA levels of caspase-3 and caspase-9 demonstrated significant differences between the groups expect group A and group E. The mRNA levels of caspase-8 had no change in all groups. The expression of caspase-3 and caspase-9 were significant differences between the groups expect group A and C, group B and D. The expression of caspase-8 had no change in all grotps.
  
  
  CONCLUSIONS
  The leaching liquids of 4 different kinds of dental alloys expect Au alloy may induce cell appotosis through mitochondrion pathway.
authors.affiliation: Dept. of Prosthodontics, Hebei United University, Tangshan, China.
authors.forename: He
authors.initials: H
authors.lastname: Meng
authors.forename: Jie
authors.initials: J
authors.lastname: Ding
authors.forename: Ren
authors.initials: R
authors.lastname: Li
authors.forename: Ruiying
authors.initials: R
authors.lastname: Liang
authors.forename: Wenhui
authors.initials: W
authors.lastname: Wu
mesh_terms: D000818:Animals; D017209:Apoptosis; D002858:Chromium Alloys; D003722:Dental Alloys; D005347:Fibroblasts; D008422:Materials Testing; D051379:Mice
chemical_list: D002858:Chromium Alloys; D003722:Dental Alloys