title: Osteoinduction of calcium phosphate biomaterials in small animals.
abstract: Although osteoinduction mechanism of calcium phosphate (CP) ceramics is still unclear, several essential properties have been reported, such as chemical composition, pore size and porosity, etc. In this study, calcium phosphate powder (Ca3(PO4)2, CaP, group 1), biphasic calcium phosphate ceramic powder (BCP, group 2), and intact BCP rods (group 3) were implanted into leg muscles of mice and dorsal muscles of rabbits. One month and three months after implantation, samples were harvested for biological and histological analysis. New bone tissues were observed in 10/10 samples in group 1, 3/10 samples in group 2, and 9/10 samples in group 3 at 3rd month in mice, but not in rabbits. In vitro, human mesenchymal stem cells (hMSCs) were cultured with trace CaP and BCP powder, and osteogenic differentiation was observed at day 7. Our results suggested that chemical composition is the prerequisite in osteoinduction, and pore structure would contribute to more bone formation.
authors.affiliation: Key Laboratory of Transplant Engineering and Immunology, Ministry of Health, West China Hospital, Sichuan University, Chengdu, People's Republic of China.
authors.forename: Lijia
authors.initials: L
authors.lastname: Cheng
authors.forename: Yujun
authors.initials: Y
authors.lastname: Shi
authors.forename: Feng
authors.initials: F
authors.lastname: Ye
authors.forename: Hong
authors.initials: H
authors.lastname: Bu
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology*; D002130:Calcium Phosphates / Q000494:pharmacology*; D002356:Cartilage / Q000166:cytology / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D002516:Ceramics / Q000494:pharmacology; D004912:Erythrocytes / Q000187:drug effects / Q000648:ultrastructure; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D006882:Hydroxyapatites / Q000494:pharmacology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008855:Microscopy, Electron, Scanning; D016348:Osseointegration / Q000187:drug effects* / Q000235:genetics; D010012:Osteogenesis / Q000187:drug effects / Q000235:genetics; D011208:Powders; D011817:Rabbits; D013194:Staining and Labeling
chemical_list: D001672:Biocompatible Materials; D002130:Calcium Phosphates; D006882:Hydroxyapatites; D011208:Powders; C074950:hydroxyapatite-beta tricalcium phosphate; C020243:calcium phosphate
keywords: Biphasic calcium phosphate ceramic; Calcium phosphate powder; Osteoinduction