title: Dengue virus photo-inactivated in presence of 1,5-iodonaphthylazide (INA) or AMT, a psoralen compound (4'-aminomethyl-trioxsalen) is highly immunogenic in mice.
abstract: Two novel methods of dengue virus inactivation using iodonaphthyl azide (INA) and aminomethyl trioxsalen (AMT) were compared with traditional virus inactivation by formaldehyde. The AMT inactivated dengue-2 virus retained its binding to a panel of 5 monoclonal antibodies specific for dengue-2 envelope protein, whereas inactivation by formaldehyde and INA led to 30-50% decrease in binding. All three inactivated viruses elicited high level virus neutralizing antibodies in vaccinated mice. However, only mice vaccinated with AMT inactivated virus mounted T cell responses similar to live, uninactivated virus.
authors.affiliation: Viral & Rickettsial Diseases Department; Naval Medical Research Center; Silver Spring, MD USA.
authors.forename: Kanakatte
authors.initials: K
authors.lastname: Raviprakash
authors.forename: Peifang
authors.initials: P
authors.lastname: Sun
authors.forename: Yossef
authors.initials: Y
authors.lastname: Raviv
authors.forename: Thomas
authors.initials: T
authors.lastname: Luke
authors.forename: Nicholas
authors.initials: N
authors.lastname: Martin
authors.forename: Tadeusz
authors.initials: T
authors.lastname: Kochel
mesh_terms: D000818:Animals; D057134:Antibodies, Neutralizing / Q000097:blood; D000914:Antibodies, Viral / Q000097:blood; D001386:Azides / Q000494:pharmacology*; D053059:Dengue Vaccines / Q000008:administration & dosage / Q000276:immunology*; D003716:Dengue Virus / Q000187:drug effects* / Q000276:immunology*; D004202:Disinfectants / Q000494:pharmacology*; D005557:Formaldehyde / Q000494:pharmacology; D008027:Light; D008807:Mice, Inbred BALB C; D013601:T-Lymphocytes / Q000276:immunology; D014307:Trioxsalen / Q000031:analogs & derivatives* / Q000494:pharmacology; D015164:Vaccines, Inactivated / Q000008:administration & dosage / Q000276:immunology; D038301:Virus Inactivation
chemical_list: D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D001386:Azides; D053059:Dengue Vaccines; D004202:Disinfectants; D015164:Vaccines, Inactivated; D005557:Formaldehyde; C015298:iodonaphthylazide; C018822:aminomethyltrioxsalen; D014307:Trioxsalen
keywords: dengue virus; immunogenicity; mouse; vaccine; virus inactivation