title: CD19 + CD23+ B cells, CD4 + CD25+ T cells, E-selectin and interleukin-12 levels in children with steroid sensitive nephrotic syndrome.
abstract: METHODS
  Soluble-lymphocyte subsets (sCD19 + CD23+ B cells and sCD4 + CD25+ T cells), soluble-adhesion molecules (sE-selectin) and interleukin-12 (sIL-12) were assayed to evaluate the pathogenesis of steroid sensitive nephrotic syndrome in 48 patients diagnosed with steroid sensitive nephrotic syndrome (SSNS) in active (AS) and remission stages (RS).
  
  
  RESULTS
  The ratios of soluble CD19 and sCD19 + CD23 increased in patients with AS with respect to the patients with RS and controls (p < 0.05). Increased sCD19 + CD23 ratios were preserved in the patients with RS when compared with the controls (p < 0.05). Moreover, the ratios of sCD4 + CD25 lymphocyte subsets were not significantly different among the groups. Similarly, serum sIL-12 levels were not considerably disparate between the AS and RS. Serum sE-selectin levels were higher in the patients with AS relative to the controls (p < 0.01) and RS (p < 0.05). No significant correlations were noted between sE-selectin and lymphocyte subset ratios, serum sIL-12 and immunoglobulin levels. There was a positive correlation between sE-selectin, triglyceride (r = 0.757, p < 0.0001) and cholesterol (r = 0.824, p < 0.0001) levels in patients with the AS.
  
  
  CONCLUSIONS
  The present results indicate that the patients with SSNS appear to have abnormalities in sCD23 + CD19+ cells, defect in T regulatory cell activity, and injury in endothelial cells as indicated by the presence high sE-selectin. These abnormalities might play a role in the pathogenesis of nephrotic syndrome. sIL-12 seems to have no role in pathogenesis of nephrotic syndrome reflecting normal Th1 response.
authors.affiliation: Department of Pediatric Nephrology, Faculty of Medicine, Eskisehir Osmangazi University, Eskisehir, TR 26480, Turkey. bilalyn@yahoo.com
authors.forename: Bilal
authors.initials: B
authors.lastname: Yildiz
authors.forename: Nuran
authors.initials: N
authors.lastname: Cetin
authors.forename: Nurdan
authors.initials: N
authors.lastname: Kural
authors.forename: Omer
authors.initials: O
authors.lastname: Colak
mesh_terms: D000293:Adolescent; D018941:Antigens, CD19 / Q000097:blood; D001402:B-Lymphocytes / Q000276:immunology*; D015415:Biomarkers / Q000097:blood*; D015496:CD4-Positive T-Lymphocytes / Q000378:metabolism; D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D002784:Cholesterol / Q000097:blood; D019040:E-Selectin / Q000097:blood*; D004730:Endothelium, Vascular / Q000187:drug effects / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005938:Glucocorticoids / Q000627:therapeutic use*; D006801:Humans; D007136:Immunoglobulins / Q000097:blood; D007155:Immunologic Factors / Q000097:blood; D018664:Interleukin-12 / Q000097:blood*; D053645:Interleukin-2 Receptor alpha Subunit / Q000097:blood; D008297:Male; D009404:Nephrotic Syndrome / Q000097:blood / Q000188:drug therapy / Q000276:immunology* / Q000473:pathology; D017455:Receptors, IgE / Q000097:blood; D013601:T-Lymphocytes / Q000276:immunology*; D014280:Triglycerides / Q000097:blood
chemical_list: D018941:Antigens, CD19; D015415:Biomarkers; D019040:E-Selectin; D005938:Glucocorticoids; D007136:Immunoglobulins; D007155:Immunologic Factors; D053645:Interleukin-2 Receptor alpha Subunit; D017455:Receptors, IgE; D014280:Triglycerides; D018664:Interleukin-12; D002784:Cholesterol