title: An automated Teflon microfluidic peptide synthesizer.
abstract: We present a microfluidic synthesizer made entirely of Teflon material for solid phase peptide synthesis (SPPS). Solvent-resistant perfluoroalkoxy (PFA) was used to construct chip-sized devices featuring multiple tri-layer pneumatic microvalves. Using these devices, model peptides were automatically synthesized and cleaved in situ in a continuous-flow manner. The total coupling and cleavage time was significantly reduced compared to conventional bulk reactors. The synthesis of a decapeptide, for instance, took less than 6 h using our device while it usually takes more than three days using conventional reactors.
authors.affiliation: National Center for Nanoscience and Technology, No.11 ZhongGuanCun BeiYiTiao, 100190 Beijing, PR China.
authors.forename: Hui
authors.initials: H
authors.lastname: Zheng
authors.forename: Weizhi
authors.initials: W
authors.lastname: Wang
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Li
authors.forename: Zihua
authors.initials: Z
authors.lastname: Wang
authors.forename: Leroy
authors.initials: L
authors.lastname: Hood
authors.forename: Christopher
authors.initials: C
authors.lastname: Lausted
authors.forename: Zhiyuan
authors.initials: Z
authors.lastname: Hu
mesh_terms: D000595:Amino Acid Sequence; D001331:Automation; D046210:Microfluidic Analytical Techniques / Q000379:methods*; D009842:Oligopeptides / Q000138:chemical synthesis* / Q000737:chemistry; D011138:Polytetrafluoroethylene / Q000737:chemistry*; D060327:Solid-Phase Synthesis Techniques / Q000379:methods*
chemical_list: D009842:Oligopeptides; D011138:Polytetrafluoroethylene