title: A facile method for the synthesis of partially O-methylated alditol acetate standards for GC-MS analysis of galactofuranose-containing structures.
abstract: Mixtures of partially O-methylated alditol acetate standards of galactofuranose were synthesized rapidly. Methyl galactofuranosides were obtained with a yield of 79.9% within 4h under optimized reaction conditions. Methylation of methyl glycosides was carried out in the presence of BaO/Ba(OH)2·8H2O, giving rise to mixtures of partially methylated glycosides. The batch containing the most diverse structures of methyl ethers was converted into partially O-methylated alditol acetates (PMAAs) and then subjected to GC-MS. These PMAAs could be used as GC-MS standards for simultaneous identification of galactofuranose units with diverse linkages in complex carbohydrates.
authors.affiliation: Department of Pharmacology, Xi'an Jiaotong University College of Medicine, Xi'an 710061, China.
authors.forename: Jian-yu
authors.initials: JY
authors.lastname: He
authors.forename: Yu-na
authors.initials: YN
authors.lastname: Guo
authors.forename: Ling-ling
authors.initials: LL
authors.lastname: Zhang
authors.forename: Lin-hong
authors.initials: LH
authors.lastname: Huang
mesh_terms: D000085:Acetates / Q000138:chemical synthesis* / Q000737:chemistry; D002236:Carbohydrate Conformation; D005690:Galactose / Q000031:analogs & derivatives / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry; D006851:Hydrochloric Acid / Q000737:chemistry; D008745:Methylation; D013402:Sugar Alcohols / Q000138:chemical synthesis* / Q000737:chemistry
chemical_list: D000085:Acetates; D013402:Sugar Alcohols; D006851:Hydrochloric Acid; D005690:Galactose
keywords: GC–MS standards; Galactofuranose-containing structures; Methylation analysis; Partially O-methylated alditol acetates