title: Involvement of unfolded protein response, p53 and Akt in modulation of porcine reproductive and respiratory syndrome virus-mediated JNK activation.
abstract: Our previous study has shown that activation of JNK plays a critical role in Porcine reproductive and respiratory syndrome virus (PRRSV)-mediated apoptosis. In this follow-up study, we further investigated the mechanisms involved in modulation of PRRSV-mediated JNK activation and apoptosis. We found that unfolded protein response (UPR) was induced in response to PRRSV infection which in turn triggered JNK activation and apoptosis. We also found that p53 and Akt were activated at the early stage of infection and functioned as negative regulator of JNK activation to counteract the PRRSV-mediated apoptosis. Furthermore, induction of UPR, p53 and Akt was not only involved in modulation of PRRSV-mediated apoptosis, but also contributed to the virus replication. Our findings indicated that multiple signaling pathways were involved in modulation of PRRSV-mediated apoptosis of the host cells via regulating JNK signaling pathway and provided novel insights into understanding the mechanisms of pathogenesis of PRRSV infection.
authors.affiliation: Division of Nutrition and Health, College of Food Science and Nutritional Engineering, China Agricultural University, no. 17 Qinghua East Road, Haidian District, Beijing 100083, China.
authors.forename: Yazhen
authors.initials: Y
authors.lastname: Huo
authors.forename: Lihong
authors.initials: L
authors.lastname: Fan
authors.forename: Shutao
authors.initials: S
authors.lastname: Yin
authors.forename: Yinhui
authors.initials: Y
authors.lastname: Dong
authors.forename: Xiao
authors.initials: X
authors.lastname: Guo
authors.forename: Hanchun
authors.initials: H
authors.lastname: Yang
authors.forename: Hongbo
authors.initials: H
authors.lastname: Hu
mesh_terms: D000818:Animals; D017209:Apoptosis; D002460:Cell Line; D002522:Chlorocebus aethiops; D020935:MAP Kinase Signaling System; D019316:Porcine respiratory and reproductive syndrome virus / Q000276:immunology* / Q000472:pathogenicity; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism*; D015398:Signal Transduction; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism*; D056811:Unfolded Protein Response
chemical_list: D016159:Tumor Suppressor Protein p53; D051057:Proto-Oncogene Proteins c-akt
keywords: Akt; Apoptosis; JNK; PRRSV; Unfolded protein response (UPR); p53