title: Identification of myb genes in Euplotes aediculatus may indicate an early evolutionary process.
abstract: The myb gene family encodes proteins characterized by a highly conserved DNA-binding domain and functions as transcription regulators. There are three myb genes in animals with three Myb-motif repeats, namely c-myb, A-myb, and B-myb. By contrast, plants possess an abundance of myb genes, the majority of which are of the type R2R3 (with two Myb-motifs). Few plant myb genes are of the type R1R2R3. Five new myb members in Euplotes were recently identified through degenerate polymerase chain reaction, which suggests that the myb gene is also a multi-member family in the unicellular protist kingdom as in other multi-cellular eukaryotes. The entire gene family further shows the presence of both R1R2R3- and R2R3-type myb. The phylogenic analysis provides concrete new evidence for the evolution of myb genes.
authors.affiliation: State Key Laboratory of Bio-membrane and Membrane Biotechnology, Institute of Zoology, Chinese Academy of Sciences, No. 1 West Beichen Road, Chaoyang District, Beijing 100101, China. Electronic address: ljh183@sohu.com.
authors.forename: Jianhua
authors.initials: J
authors.lastname: Lv
authors.forename: Tie
authors.initials: T
authors.lastname: Yang
authors.forename: Haixia
authors.initials: H
authors.lastname: Yang
authors.forename: Zhiwei
authors.initials: Z
authors.lastname: Li
authors.forename: Peiwu
authors.initials: P
authors.lastname: Qin
authors.forename: Xiling
authors.initials: X
authors.lastname: Zhang
authors.forename: Xiaoliang
authors.initials: X
authors.lastname: Liang
authors.forename: Jiayin
authors.initials: J
authors.lastname: Li
authors.forename: Quan
authors.initials: Q
authors.lastname: Chen
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D005075:Biological Evolution; D016812:Euplotes / Q000235:genetics*; D020570:Genes, myb; D015698:Genomic Library; D006801:Humans; D008969:Molecular Sequence Data; D005810:Multigene Family; D020626:Oncogene Proteins v-myb / Q000145:classification / Q000235:genetics*; D010802:Phylogeny; D010944:Plants / Q000235:genetics; D015800:Protozoan Proteins / Q000145:classification / Q000235:genetics*; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
chemical_list: D020626:Oncogene Proteins v-myb; D015800:Protozoan Proteins
keywords: AP; Ciliates; DEPC; E. aediculatus; Euplotes aediculatus; MAC; ORF; PCR; RACE; Unicellular; adaptor primer; diethylpyrocarbonate; macronuclear; myb gene; open reading frame; polymerase chain reaction; random amplification of cDNA ends