title: Ultrasensitive telomerase activity detection by telomeric elongation controlled surface enhanced Raman scattering.
abstract: Telomerase is now considered to be a valuable biomarker and therapeutic target in the diagnosis and treatment of cancerous diseases, which brings an urgent need in the development of fast and efficient telomerase detection strategies. Here, a new surface enhanced Raman scattering (SERS) based protocol using telomeric elongation controlled SERS (TEC-SERS) effect for the ultrasensitive telomerase detection is presented. The TEC-SERS protocol not only provides an unprecedented high sensitivity but also avoids laborious PCR procedures. The detection limit is ≈2-3 orders of magnitude lower than those of previously reported methods. This highly sensitive and straightforward TEC-SERS protocol can be developed as a routine telomerase detection method, which would greatly facilitate the telomerase based ultra-early diagnosis of malignant tumors and the fast screening of anti-cancer drugs.
authors.affiliation: Advanced Photonics Center, Southeast University, Nanjing 210096, China.
authors.forename: Shenfei
authors.initials: S
authors.lastname: Zong
authors.forename: Zhuyuan
authors.initials: Z
authors.lastname: Wang
authors.forename: Hui
authors.initials: H
authors.lastname: Chen
authors.forename: Yiping
authors.initials: Y
authors.lastname: Cui
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry; D045744:Cell Line, Tumor; D005347:Fibroblasts / Q000378:metabolism; D006046:Gold / Q000737:chemistry; D006367:HeLa Cells; D006801:Humans; D061986:MCF-7 Cells; D053768:Metal Nanoparticles / Q000737:chemistry; D046529:Microscopy, Electron, Transmission; D009369:Neoplasms / Q000175:diagnosis*; D009360:Neoplastic Cells, Circulating; D012680:Sensitivity and Specificity; D013059:Spectrum Analysis, Raman; D020349:Surface Plasmon Resonance; D013499:Surface Properties; D019098:Telomerase / Q000378:metabolism / Q000648:ultrastructure*
chemical_list: D000970:Antineoplastic Agents; D006046:Gold; D019098:Telomerase
keywords: biosensors; nanoparticles; sensitivity; surface enhanced Raman scattering; telomerase