title: KLF6 and iNOS regulates apoptosis during respiratory syncytial virus infection.
abstract: Human respiratory syncytial virus (RSV) is a highly pathogenic lung-tropic virus that causes severe respiratory diseases. Enzymatic activity of inducible nitric oxide (iNOS) is required for NO generation. Although NO contributes to exaggerated lung disease during RSV infection, the role of NO in apoptosis during infection is not known. In addition, host trans-activator(s) required for iNOS gene expression during RSV infection is unknown. In the current study we have uncovered the mechanism of iNOS gene induction by identifying kruppel-like factor 6 (KLF6) as a critical transcription factor required for iNOS gene expression during RSV infection. Furthermore, we have also uncovered the role of iNOS as a critical host factor regulating apoptosis during RSV infection.
authors.affiliation: Department of Microbiology and Immunology, The University of Texas Health Science Center at San Antonio, San Antonio, TX 78229, United States.
authors.forename: Victoria
authors.initials: V
authors.lastname: Mgbemena
authors.forename: Jesus
authors.initials: J
authors.lastname: Segovia
authors.forename: Te-Hung
authors.initials: TH
authors.lastname: Chang
authors.forename: Santanu
authors.initials: S
authors.lastname: Bose
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D047369:Chromatin Immunoprecipitation; D006801:Humans; D000075723:Kruppel-Like Factor 6; D051741:Kruppel-Like Transcription Factors / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D052247:Nitric Oxide Synthase Type II / Q000235:genetics / Q000378:metabolism*; D011518:Proto-Oncogene Proteins / Q000235:genetics / Q000378:metabolism*; D018357:Respiratory Syncytial Virus Infections / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015533:Transcriptional Activation
chemical_list: C105465:KLF6 protein, human; D000075723:Kruppel-Like Factor 6; D051741:Kruppel-Like Transcription Factors; D011518:Proto-Oncogene Proteins; C496319:NOS2 protein, human; D052247:Nitric Oxide Synthase Type II
keywords: Apoptosis; BMDMs; ChIP; Inducible nitric oxide synthase; KLF6; KO; Kruppel-like factor 6; L-NIL; N(6)-(1-iminoethyl)-l-lysine dihydrochloride; NO; Nitric oxide; RSV; Respiratory syncytial virus; Transcription factor; WT; bone marrow-derived macrophages; chromatin immuno-precipitation; iNOS; inducible nitric oxide synthase; knock-out; kruppel-like factor 6; nitric oxide; respiratory syncytial virus; wild type