title: Determination of five active compounds in Artemisia princeps and A. capillaris based on UPLC-DAD and discrimination of two species with multivariate analysis.
abstract: Five active compounds, chlorogenic acid, 3,5-di-O-caffeoylquinic acid, 4,5-di-O-caffeoylquinic acid, jaceosidin, and eupatilin, in Artemisia princeps (Compositae) were simultaneously determined by ultra-performance liquid chromatography connected to diode array detector. The morphological resemblance between A. princeps and A. capillaris makes it difficult to properly identify species properly. It occasionally leads to misuse or misapplication in Korean traditional medicine. In the study, the discrimination between A. princeps and A. capillaris was optimally performed by the developed validation method, which resulted in definitely a difference between two species. Also, it was developed the most reliable markers contributing to the discrimination of two species by the multivariate analysis methods, such as a principal component analysis and a partial least squares discrimination analysis.
authors.affiliation: College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University, San 56-1, Sillim-Dong, Daehak-Dong, Gwanak-Gu, Seoul, 151-742, Republic of Korea.
authors.forename: Heejung
authors.initials: H
authors.lastname: Yang
authors.forename: Dong Young
authors.initials: DY
authors.lastname: Lee
authors.forename: Minji
authors.initials: M
authors.lastname: Jeon
authors.forename: Youngbae
authors.initials: Y
authors.lastname: Suh
authors.forename: Sang Hyun
authors.initials: SH
authors.lastname: Sung
mesh_terms: D019306:Artemisia / Q000737:chemistry* / Q000145:classification*; D002138:Calibration; D002726:Chlorogenic Acid / Q000032:analysis; D002853:Chromatography, Liquid / Q000592:standards; D016002:Discriminant Analysis; D005419:Flavonoids / Q000032:analysis; D016018:Least-Squares Analysis; D055670:Medicine, Korean Traditional; D009005:Monosaccharides / Q000032:analysis; D015999:Multivariate Analysis; D008517:Phytotherapy; D010936:Plant Extracts / Q000032:analysis*; D010946:Plants, Medicinal; D025341:Principal Component Analysis; D011801:Quinic Acid / Q000031:analogs & derivatives / Q000032:analysis; D012015:Reference Standards; D015203:Reproducibility of Results; D013045:Species Specificity
chemical_list: C500585:3,5-di-O-(E)-caffeoylquinic acid; C480201:4,5-dicaffeoyl quinic acid; D005419:Flavonoids; D009005:Monosaccharides; D010936:Plant Extracts; D011801:Quinic Acid; C477508:jaceosidin; D002726:Chlorogenic Acid; C045325:eupatilin