title: Sprouty2 but not Sprouty4 is a potent inhibitor of cell proliferation and migration of osteosarcoma cells.
abstract: As negative regulators of receptor tyrosine kinase-mediated signalling, Sprouty proteins fulfil important roles during carcinogenesis. In this report, we demonstrate that Sprouty2 protein expression inhibits cell proliferation and migration in osteosarcoma-derived cells. Although earlier reports describe a tumour-promoting function, these results indicate that Sprouty proteins also have the potential to function as tumour suppressors in sarcoma. In contrast to Sprouty2, Sprouty4 expression failed to interfere with proliferation and migration of the osteosarcoma-derived cells, possibly due to a less pronounced interference with mitogen-activated protein kinase activity. Sequences within the NH2-terminus are responsible for the specific inhibitory function of Sprouty2 protein.
authors.affiliation: Institute of Cancer Research, Department of Medicine I, Medical University of Vienna, Borschkegasse 8a, A-1090 Vienna, Austria.
authors.forename: Nadine
authors.initials: N
authors.lastname: Rathmanner
authors.forename: Barbara
authors.initials: B
authors.lastname: Haigl
authors.forename: Vanita
authors.initials: V
authors.lastname: Vanas
authors.forename: Angelina
authors.initials: A
authors.lastname: Doriguzzi
authors.forename: Andrea
authors.initials: A
authors.lastname: Gsur
authors.forename: Hedwig
authors.initials: H
authors.lastname: Sutterlüty-Fall
mesh_terms: D001859:Bone Neoplasms / Q000378:metabolism*; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D015972:Gene Expression Regulation, Neoplastic; D016147:Genes, Tumor Suppressor; D005982:Glutathione Transferase / Q000378:metabolism; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000378:metabolism*; D020935:MAP Kinase Signaling System; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D012516:Osteosarcoma / Q000378:metabolism*; D054730:Protein Interaction Domains and Motifs; D015398:Signal Transduction
chemical_list: D047908:Intracellular Signaling Peptides and Proteins; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; C485561:SPRY2 protein, human; C499038:SPRY4 protein, human; D005982:Glutathione Transferase
keywords: AA; FCS; GST; MAPK; Migration; Osteosarcoma; PAGE; PLC; Proliferation; RTK; Signalling; Sprouty; Spry; aminoacids; fetal calf serum; glutathione S-transferase; mitogen activated protein kinase; pERK; phospholipase C; phosphorylated extracellular signal-regulated kinase; phosporylated ribosomal protein S6; polyacrylamidgelelectrophoresis; prPS6; receptor tyrosine kinase