title: Peroxiredoxins and sulfiredoxin at the crossroads of the NO and H2O2 signaling pathways.
abstract: Peroxiredoxins (Prxs) are a family of peroxidases that maintain thiol homeostasis by catalyzing the reduction of organic hydroperoxides, H2O2, and peroxynitrite. Eukaryotic 2-Cys-Prxs, also referred to as typical Prxs, can be inactivated by oxidation of the catalytic cysteine to sulfinic acid, which may regulate the intracellular messenger function of H2O2. A small redox protein, sulfiredoxin (Srx), has been shown to reduce sulfinylated 2-Cys-Prxs and thus to regenerate active 2-Cys-Prxs. We previously reported that cytokine-induced nitric oxide (NO) intervenes in this pathway by decreasing the level of 2-Cys overoxidation and by upregulating Srx through the activation of the transcription factor nuclear factor erythroid 2-related factor (Nrf2). Here, we describe the methods used to monitor the interplay between NO and H2O2 in the regulation of the Prx/Srx system in immunostimulated macrophages, which produce both reactive oxygen species and NO.
authors.affiliation: Institut de Chimie des Substances Naturelles, Centre National de la Recherche Scientifique, Gif-sur-Yvette, France.
authors.forename: Kahina
authors.initials: K
authors.lastname: Abbas
authors.forename: Sylvie
authors.initials: S
authors.lastname: Riquier
authors.forename: Jean-Claude
authors.initials: JC
authors.lastname: Drapier
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002460:Cell Line; D003470:Culture Media; D020536:Enzyme Activators / Q000494:pharmacology; D005949:Glucose Oxidase / Q000737:chemistry; D018398:Homeodomain Proteins / Q000378:metabolism*; D006861:Hydrogen Peroxide / Q000378:metabolism*; D008264:Macrophages / Q000201:enzymology; D051379:Mice; D009569:Nitric Oxide / Q000378:metabolism*; D010084:Oxidation-Reduction; D050862:Oxidoreductases Acting on Sulfur Group Donors / Q000378:metabolism*; D011499:Protein Processing, Post-Translational; D015398:Signal Transduction; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
chemical_list: D003470:Culture Media; D020536:Enzyme Activators; D018398:Homeodomain Proteins; C489936:Prrx1 protein, mouse; D009569:Nitric Oxide; D006861:Hydrogen Peroxide; D005949:Glucose Oxidase; D050862:Oxidoreductases Acting on Sulfur Group Donors; C529381:sulfiredoxin protein, mouse; D013755:Tetradecanoylphorbol Acetate
keywords: Hydrogen peroxide; Macrophages; Nitric oxide; Nrf2; Peroxiredoxins; Sulfiredoxin