title: Electrochemical analysis of the UV treated bactericidal Ti6Al4V surfaces.
abstract: This research investigates in detail the bactericidal effect exhibited by the surface of the biomaterial Ti6Al4V after being subjected to UV-C light. It has been recently hypothesized that small surface currents, occurring as a consequence of the electron-hole pair recombination taking place after the excitation process, are behind the bactericidal properties displayed by this UV-treated material. To corroborate this hypothesis we have used different electrochemical techniques, such as electrochemical impedance spectroscopy (EIS), potentiodynamic polarization plots and Mott-Schottky plots. EIS and Mott-Schottky plots have shown that UV-C treatment causes an initial increase on the surface electrical conduction of this material. In addition, EIS and polarization plots demonstrated that higher corrosion currents occur at the UV treated than at the non-irradiated samples. Despite this increase in the corrosion currents, EIS has also shown that such currents are not likely to affect the good stability of this material oxide film since the irradiated samples completely recovered the control values after being stored in dark conditions for a period not longer than 24h. These results agree with the already-published in vitro transitory behavior of the bactericidal effect, which was shown to be present at initial times after the biomaterial implantation, a crucial moment to avoid a large number of biomaterial associated infections.
authors.affiliation: Networking Research Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Spain.
authors.forename: Miguel A
authors.initials: MA
authors.lastname: Pacha-Olivenza
authors.forename: Amparo M
authors.initials: AM
authors.lastname: Gallardo-Moreno
authors.forename: Virginia
authors.initials: V
authors.lastname: Vadillo-Rodríguez
authors.forename: M Luisa
authors.initials: ML
authors.lastname: González-Martín
authors.forename: Ciro
authors.initials: C
authors.lastname: Pérez-Giraldo
authors.forename: Juan C
authors.initials: JC
authors.lastname: Galván
mesh_terms: D000497:Alloys; D000900:Anti-Bacterial Agents / Q000494:pharmacology* / Q000528:radiation effects*; D001419:Bacteria / Q000187:drug effects / Q000528:radiation effects; D001422:Bacterial Adhesion / Q000187:drug effects / Q000528:radiation effects; D058266:Dielectric Spectroscopy; D004563:Electrochemistry / Q000379:methods*; D050296:Microbial Viability / Q000187:drug effects / Q000528:radiation effects; D013499:Surface Properties; D014025:Titanium / Q000494:pharmacology* / Q000528:radiation effects*; D014466:Ultraviolet Rays
chemical_list: D000497:Alloys; D000900:Anti-Bacterial Agents; C031462:titanium alloy (TiAl6V4); D014025:Titanium
keywords: EIS; Mott–Schottky curves; Potentiodynamic polarization plots; Ti6Al4V; Ultraviolet