title: The effect of hematocrit on bioanalysis of DBS: results from the EBF DBS-microsampling consortium.
abstract: BACKGROUND
  The European Bioanalysis Forum dried blood spots (DBS)/microsampling consortium is reporting back from the experiments they performed on further documenting the potential hurdles of the DBS technology. This paper is focused on the impact of hematocrit changes on DBS analyses.
  
  
  RESULTS
  The hematocrit can have an effect on the size of the blood spot, on spot homogeneity and on extraction recovery in a compound-dependent manner. The extraction recovery can change upon aging in an hematocrit-dependent way. Different card materials can give different outcomes.
  
  
  CONCLUSIONS
  The results from the conducted experiments show that the issues of DBS in regulated bioanalysis are real and that the technology will need improvements to be ready for use as a general tool for regulated bioanalysis.
authors.affiliation: Janssen R&D NV, Turnhoutseweg 30, B-2340 Beerse, Belgium. rdvries@its.jnj.com
authors.forename: Ronald
authors.initials: R
authors.lastname: de Vries
authors.forename: Matthew
authors.initials: M
authors.lastname: Barfield
authors.forename: Nico
authors.initials: N
authors.lastname: van de Merbel
authors.forename: Bernhard
authors.initials: B
authors.lastname: Schmid
authors.forename: Christoph
authors.initials: C
authors.lastname: Siethoff
authors.forename: Jordi
authors.initials: J
authors.lastname: Ortiz
authors.forename: Elwin
authors.initials: E
authors.lastname: Verheij
authors.forename: Ben
authors.initials: B
authors.lastname: van Baar
authors.forename: Zoe
authors.initials: Z
authors.lastname: Cobb
authors.forename: Steve
authors.initials: S
authors.lastname: White
authors.forename: Philip
authors.initials: P
authors.lastname: Timmerman
mesh_terms: D059788:Dried Blood Spot Testing / Q000592:standards* / Q000706:statistics & numerical data; D005060:Europe; D005161:Facility Regulation and Control; D006400:Hematocrit / Q000592:standards*; D006801:Humans; D007193:Independent Practice Associations; D015203:Reproducibility of Results; D052617:Solid Phase Microextraction; D012997:Solvents; D013048:Specimen Handling / Q000592:standards*; D053719:Tandem Mass Spectrometry
chemical_list: D012997:Solvents