title: In vivo imaging of glucose uptake and metabolism in tumors.
abstract: Tumors have a greater reliance on anaerobic glycolysis for energy production than normal tissues. We developed a noninvasive method for imaging glucose uptake in vivo that is based on magnetic resonance imaging and allows the uptake of unlabeled glucose to be measured through the chemical exchange of protons between hydroxyl groups and water. This method differs from existing molecular imaging methods because it permits detection of the delivery and uptake of a metabolically active compound in physiological quantities. We show that our technique, named glucose chemical exchange saturation transfer (glucoCEST), is sensitive to tumor glucose accumulation in colorectal tumor models and can distinguish tumor types with differing metabolic characteristics and pathophysiologies. The results of this study suggest that glucoCEST has potential as a useful and cost-effective method for characterizing disease and assessing response to therapy in the clinic.
authors.affiliation: University College London Centre for Advanced Biomedical Imaging, Division of Medicine and Institute of Child Health, London, UK. simon.walkersamuel@ucl.ac.uk
authors.forename: Simon
authors.initials: S
authors.lastname: Walker-Samuel
authors.forename: Rajiv
authors.initials: R
authors.lastname: Ramasawmy
authors.forename: Francisco
authors.initials: F
authors.lastname: Torrealdea
authors.forename: Marilena
authors.initials: M
authors.lastname: Rega
authors.forename: Vineeth
authors.initials: V
authors.lastname: Rajkumar
authors.forename: S Peter
authors.initials: SP
authors.lastname: Johnson
authors.forename: Simon
authors.initials: S
authors.lastname: Richardson
authors.forename: Miguel
authors.initials: M
authors.lastname: Gonçalves
authors.forename: Harold G
authors.initials: HG
authors.lastname: Parkes
authors.forename: Erik
authors.initials: E
authors.lastname: Arstad
authors.forename: David L
authors.initials: DL
authors.lastname: Thomas
authors.forename: R Barbara
authors.initials: RB
authors.lastname: Pedley
authors.forename: Mark F
authors.initials: MF
authors.lastname: Lythgoe
authors.forename: Xavier
authors.initials: X
authors.lastname: Golay
mesh_terms: D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D001345:Autoradiography; D002247:Carbon Isotopes; D015687:Cell Hypoxia; D005260:Female; D019788:Fluorodeoxyglucose F18; D005947:Glucose / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D008279:Magnetic Resonance Imaging; D051379:Mice; D008856:Microscopy, Fluorescence; D009369:Neoplasms / Q000378:metabolism* / Q000473:pathology; D010477:Perfusion; D023041:Xenograft Model Antitumor Assays
chemical_list: D000596:Amino Acids; D002247:Carbon Isotopes; D019788:Fluorodeoxyglucose F18; D005947:Glucose