title: The endoplasmic reticulum-quality control component SDF2 is essential for XA21-mediated immunity in rice.
abstract: Plant genomes contain large number of plasma membrane (PM)-localized immune receptors, also called pattern recognition receptors (PRRs). PRRs are synthesized in the endoplasmic reticulum (ER) and then translocated to the PM, where they recognize conserved pathogen-associated molecular patterns (PAMPs) and activate innate immune response. The rice XA21 immune receptor confers resistance to the Gram-negative bacterial pathogen, Xanthomonas oryzae pv. oryzae (Xoo). To identify components that mediate XA21-mediated signaling, we performed co-purification experiments using C-terminal GFP tagged XA21 protein. Several endoplasmic reticulum-quality control (ER-QC) proteins including stromal-derived factor 2 (SDF2) co-purified with XA21. Silencing of the SDF2 genes in the XA21 rice genetic background compromises resistance to Xoo but does not affect plant growth and development.
authors.affiliation: Department of Plant Pathology and The Genome Center, University of California Davis, Davis, CA, United States.
authors.forename: Chang-Jin
authors.initials: CJ
authors.lastname: Park
authors.forename: Rita
authors.initials: R
authors.lastname: Sharma
authors.forename: Benoit
authors.initials: B
authors.lastname: Lefebvre
authors.forename: Patrick E
authors.initials: PE
authors.lastname: Canlas
authors.forename: Pamela C
authors.initials: PC
authors.lastname: Ronald
mesh_terms: D000595:Amino Acid Sequence; D004721:Endoplasmic Reticulum / Q000378:metabolism; D015870:Gene Expression; D018506:Gene Expression Regulation, Plant; D020868:Gene Silencing; D018745:Genome, Plant / Q000235:genetics*; D008969:Molecular Sequence Data; D012275:Oryza / Q000166:cytology / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D010935:Plant Diseases / Q000276:immunology*; D057865:Plant Immunity; D018515:Plant Leaves / Q000166:cytology / Q000235:genetics / Q000276:immunology / Q000382:microbiology; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism; D051192:Receptors, Pattern Recognition / Q000235:genetics / Q000378:metabolism; D011993:Recombinant Fusion Proteins; D016415:Sequence Alignment; D015398:Signal Transduction; D013385:Succinate Dehydrogenase / Q000235:genetics / Q000378:metabolism; D014974:Xanthomonas / Q000472:pathogenicity / Q000502:physiology*
chemical_list: D010940:Plant Proteins; D051192:Receptors, Pattern Recognition; D011993:Recombinant Fusion Proteins; C097148:Xa21 protein, Oryza sativa; D013385:Succinate Dehydrogenase; D017346:Protein Serine-Threonine Kinases
keywords: ER-QC; LRRs; PM; PR; PRR; Receptor-like kinase; SDF2; XA21; Xanthomonas oryzae pv. oryzae; Xanthomonas resistance 21; Xoo; endoplasmic reticulum-quality control; leucine-rich repeats; pathogenesis-related; pattern recognition receptor; plasma membrane; stromal-derived factor 2