title: Proteins differentially expressed in conidia and mycelia of the entomopathogenic fungus Metarhizium anisopliae sensu stricto.
abstract: Metarhizium anisopliae is a well-characterized entomopathogenic fungus that attacks a variety of insects. Its conidia are involved in its propagation and also in its infection of host insects. To investigate the protein expression profiles and to identify the proteins related to development and pathogenesis, we performed a comparative proteomic analysis of the conidia and mycelia of an M. anisopliae strain (Ma1291). The analysis used 2-dimensional gel electrophoresis and matrix-assisted laser desorption ionization time-of-flight mass spectrometry. We detected 898 ± 37 protein spots in conidia and 1072 ± 24 in mycelia of strain Ma1291. A comparison of the 2 protein-expression profiles indicated that only 28% of protein spots were common to both developmental stages. Finally, we identified 30 proteins (19 from conidia and 11 from mycelia). The identified proteins exclusive to conidia were those involved in protective processes, appressorium formation, and degradation of the host cuticle (protease PR1H). The identified proteins exclusive to mycelia included major proteins participating in biosynthetic and energy metabolism, such as UTP-glucose-1-phosphate uridylyltransferase and heat shock protein 70. This research provides the first proteomic analysis of different developmental stages of M. anisopliae, and the results should facilitate clarification of the molecular basis of these epigenetic variations.
authors.affiliation: Key Laboratory of Biopesticide and Chemical Biology, Ministry of Education, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, People's Republic of China.
authors.forename: Yubin
authors.initials: Y
authors.lastname: Su
authors.forename: Qingfeng
authors.initials: Q
authors.lastname: Guo
authors.forename: Jie
authors.initials: J
authors.lastname: Tu
authors.forename: Xiaoxia
authors.initials: X
authors.lastname: Li
authors.forename: Lixue
authors.initials: L
authors.lastname: Meng
authors.forename: Liping
authors.initials: L
authors.lastname: Cao
authors.forename: Dong
authors.initials: D
authors.lastname: Dong
authors.forename: Junzhi
authors.initials: J
authors.lastname: Qiu
authors.forename: Xiong
authors.initials: X
authors.lastname: Guan
mesh_terms: D000818:Animals; D015180:Electrophoresis, Gel, Two-Dimensional; D005656:Fungal Proteins / Q000032:analysis* / Q000378:metabolism; D007313:Insecta / Q000382:microbiology*; D052981:Metarhizium / Q000737:chemistry* / Q000254:growth & development* / Q000378:metabolism / Q000472:pathogenicity; D025282:Mycelium / Q000737:chemistry*; D040901:Proteomics; D013172:Spores, Fungal / Q000737:chemistry*; D014774:Virulence
chemical_list: D005656:Fungal Proteins