title: Can thrombin-activated platelet releasate compensate the age-induced decrease in cell proliferation of MSC?
abstract: Mesenchymal progenitor cells (MSCs) are promising for cell-based regeneration therapies. In elderly patients a reduced proliferation of MSCs has been described. Platelet-rich plasma (PRP) contains important factors necessary for osteogenic regeneration. The aim of this study was to find out whether the age-induced decrease in cell proliferation can be compensated by the use of supernatant of centrifuged, activated PRP (tPR). MSCs of donors of three age groups (A: young, 14-16 years, B: middle age, 36-46 years, C: older, 74-83 years) were expanded with 20% FCS alone or supplemented with thrombin-activated platelet releasate (tPR) (1%, 2.5%, and 5%) or platelet-poor plasma (PPP 5%). Cell proliferation and differentiation was measured on days 0, 3, and 7. Proliferation increased significantly in groups A and B with tPR, and non-significantly in group C. The generation times of MSCs of elderly patients were significantly increased in group C compared to groups A and B. Addition of 1% or 2.5% tPR significantly reduced population doubling times of all age groups. Adding tPR stimulates the proliferation rate of MSCs independent of donor age. For juvenile and middle-aged patients this influence was significant. Cells differentiation into osteoblasts was not influenced by addition of tPR.
authors.affiliation: Department of Orthopaedics, University Hospital, Heinrich-Heine-University, Düsseldorf, Germany.
authors.forename: Melanie
authors.initials: M
authors.lastname: Vogl
authors.forename: Johannes
authors.initials: J
authors.lastname: Fischer
authors.forename: Marcus
authors.initials: M
authors.lastname: Jäger
authors.forename: Christoph
authors.initials: C
authors.lastname: Zilkens
authors.forename: Rüdiger
authors.initials: R
authors.lastname: Krauspe
authors.forename: Monika
authors.initials: M
authors.lastname: Herten
mesh_terms: D000293:Adolescent; D000328:Adult; D000367:Age Factors; D000368:Aged; D000369:Aged, 80 and over; D000469:Alkaline Phosphatase / Q000378:metabolism; D001792:Blood Platelets / Q000187:drug effects / Q000502:physiology*; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000502:physiology*; D008875:Middle Aged; D015539:Platelet Activation; D013917:Thrombin / Q000494:pharmacology*
chemical_list: D000469:Alkaline Phosphatase; D013917:Thrombin
keywords: age-induced decrease; mesenchymal progenitor cells (MSC); platelet releasate (PR); platelet-rich plasma (PRP); thrombin