title: [Evaluation of specific antibodies of blood receiver sera to Treponema pallidum by chemiluminescence immunoassay].
abstract: OBJECTIVE
  The purpose of this study was to evaluate the diagnostic performance of chemiluminescence immunoassay (CLIA ) , in comparison with that of the following currently used treponemal tests: hemagglutination test (TPHA), and Western Blot (WB).
  
  
  METHODS
  First, a retrospective study was performed with a panel of 18 494 blood receiver sera by CLIA and TPHA, the specific antibody against T. pallidum in 177 were positive sera by CLIA and/or TPHA, 81 clinical and serologically characterized syphilitic sera, 55 sera obtained from subjects with potentially interfering diseases, and 250 healthy sera were negative were detected by CLIA, TPHA and WB.
  
  
  RESULTS
  The results of WB as the gold standard, the sensitivity of CLIA (98. 4%) was significantly higher than that of TPHA (94. 4%) (Chi2 = 5.76,P <0. 05), the specificity of CLIA (100%) was similar to that of TPHA (99.7%) (Chi2 =1. 0, P >0. 05).
  
  
  CONCLUSIONS
  CLIA is characterized with higher sensitivity and specificity, It is suitable for screening Syphilis in clinical laboratory.
authors.affiliation: Department of Blood Transfusion, the First Hospital of Jiaxing City in Zhejiang Province, Zhejiang 314001, China
authors.forename: Xue-chun
authors.initials: XC
authors.lastname: Wang
authors.forename: Ling-zhi
authors.initials: LZ
authors.lastname: Chen
authors.forename: Qin
authors.initials: Q
authors.lastname: Yu
authors.forename: Ai-hua
authors.initials: AH
authors.lastname: Sun
mesh_terms: D000907:Antibodies, Bacterial / Q000097:blood* / Q000276:immunology; D015153:Blotting, Western / Q000379:methods; D016022:Case-Control Studies; D006386:Hemagglutination Tests / Q000379:methods; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D049449:Luminescence; D008163:Luminescent Measurements / Q000379:methods*; D012189:Retrospective Studies; D012680:Sensitivity and Specificity; D013587:Syphilis / Q000097:blood* / Q000382:microbiology*; D014210:Treponema pallidum / Q000276:immunology*
chemical_list: D000907:Antibodies, Bacterial