title: Aspergillus fumigatus hyphal damage caused by noninvasive radiofrequency field-induced hyperthermia.
abstract: We studied the effect of noninvasive radiofrequency-induced hyperthermia on the viability of Aspergillus fumigatus hyphae in vitro. Radiofrequency-induced hyperthermia resulted in significant (>70%, P < 0.0001) hyphal damage in a time and thermal dose-dependent fashion as assessed by XTT [(sodium 2,3,-bis(2-methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino)-carbonyl] (1)-2H-tetrazolium inner salt)], DiBAC [bis-(1,3-dibutylbarbituric acid) trimethine oxonol] staining, and transmission electron microscopy. For comparison, water bath hyperthermia was used over the range of 45 to 55°C to study hyphal damage. Radiofrequency-induced hyperthermia resulted in severe damage to the outer fibrillar layer of hyphae at a shorter treatment time compared to water bath hyperthermia. Our preliminary data suggest that radiofrequency-induced hyperthermia might be an additional therapeutic approach to use in the management of mold infections.
authors.affiliation: Department of Surgical Oncology, The University of Texas M.D. Anderson Cancer Center, Houston, Texas, USA.
authors.forename: Warna D
authors.initials: WD
authors.lastname: Kaluarachchi
authors.forename: Brandon T
authors.initials: BT
authors.lastname: Cisneros
authors.forename: Stuart J
authors.initials: SJ
authors.lastname: Corr
authors.forename: Nathaniel D
authors.initials: ND
authors.lastname: Albert
authors.forename: Steven A
authors.initials: SA
authors.lastname: Curley
authors.forename: Dimitrios P
authors.initials: DP
authors.lastname: Kontoyiannis
mesh_terms: D001232:Aspergillus fumigatus / Q000254:growth & development / Q000648:ultrastructure*; D001463:Barbiturates; D005456:Fluorescent Dyes; D006358:Hot Temperature; D025301:Hyphae / Q000254:growth & development / Q000648:ultrastructure*; D007555:Isoxazoles; D050296:Microbial Viability; D011846:Radio Waves; D013778:Tetrazolium Salts
chemical_list: D001463:Barbiturates; D005456:Fluorescent Dyes; D007555:Isoxazoles; D013778:Tetrazolium Salts; C052795:bis(1,3-dibutylbarbiturate)trimethine oxonol; C059087:2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-5-((phenylamino)carbonyl)-2H-tetrazolium hydroxide