title: In vitro and in vivo anti-inflammatory effects of IMMLG5521, a coumarin derivative.
abstract: Several coumarin derivatives have been reported to present multiple biological activities. In this study, in vitro the compound IMMLG5521 (0.1, 1, 10μM) can inhibit the release of β-glucuronidase from PAF-stimulated polymorphonuclear leukocytes, the compound IMMLG5521 (0.1, 1, 10μM) can inhibit NO production and decrease TNF-α and IL-β release from LPS-stimulated RAW264.7 cells. In vivo, we evaluated the effect of IMMLG5521 on acute and chronic inflammation models. Our data showed that IMMLG5521 (6mg/kg, 12mg/kg) could inhibit xylene-induced ear swelling and cotton pellet-induced granuloma formation in mice. Taken together, the compound IMMLG5521 inhibited the release of inflammatory factors and mediators in vitro, decreased inflammation response in mice. The compound IMMLG5521 can inhibit inflammation in vitro and in vivo.
authors.affiliation: Institute of Materia Medica, Chinese Academy of Medical Sciences, Beijing, China.
authors.forename: Zhipeng
authors.initials: Z
authors.lastname: Li
authors.forename: Jinfeng
authors.initials: J
authors.lastname: Hu
authors.forename: Mingna
authors.initials: M
authors.lastname: Sun
authors.forename: Xiuyun
authors.initials: X
authors.lastname: Song
authors.forename: Gang
authors.initials: G
authors.lastname: Li
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.forename: Guangyan
authors.initials: G
authors.lastname: Li
authors.forename: Haijie
authors.initials: H
authors.lastname: Ji
authors.forename: Gang
authors.initials: G
authors.lastname: Liu
authors.forename: Naihong
authors.initials: N
authors.lastname: Chen
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000008:administration & dosage*; D002478:Cells, Cultured; D003374:Coumarins / Q000008:administration & dosage* / Q000138:chemical synthesis; D003877:Dermatitis, Contact / Q000188:drug therapy* / Q000276:immunology; D004423:Ear / Q000473:pathology; D005966:Glucuronidase / Q000378:metabolism; D006099:Granuloma / Q000188:drug therapy* / Q000276:immunology; D053583:Interleukin-1beta / Q000378:metabolism; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D009504:Neutrophils / Q000187:drug effects* / Q000276:immunology; D051381:Rats; D011919:Rats, Inbred Strains; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D003374:Coumarins; C561127:IMMLG5521; D053583:Interleukin-1beta; D014409:Tumor Necrosis Factor-alpha; D005966:Glucuronidase
keywords: Granuloma; Inflammation; Polymorphonuclear leukocytes; Swelling; β-Glucuronidase