title: Generation of affinity-tagged fluoromycobacteriophages by mixed assembly of phage capsids.
abstract: Addition of affinity tags to bacteriophage particles facilitates a variety of applications, including vaccine construction and diagnosis of bacterial infections. Addition of tags to phage capsids is desirable, as modification of the tails can lead to poor adsorption and loss of infectivity. Although tags can readily be included as fusions to head decoration proteins, many phages do not have decoration proteins as virion components. The addition of a small (10-amino-acid) Strep-tag II (STAG II) to the mycobacteriophage TM4 capsid subunit, gp9, was not tolerated as a genetically homogenous recombinant phage but could be incorporated into the head by growth of wild-type phage on a host expressing the capsid-STAG fusion. Particles with capsids composed of wild-type and STAG-tagged subunit mixtures could be grown to high titers, showed good infectivities, and could be used to isolate phage-bacterium complexes. Preparation of a STAG-labeled fluoromycobacteriophage enabled capture of bacterial complexes and identification of infected bacteria by fluorescence.
authors.affiliation: Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania, USA.
authors.forename: Mariana
authors.initials: M
authors.lastname: Piuri
authors.forename: Liliana
authors.initials: L
authors.lastname: Rondón
authors.forename: Estefanía
authors.initials: E
authors.lastname: Urdániz
authors.forename: Graham F
authors.initials: GF
authors.lastname: Hatfull
mesh_terms: D001431:Bacteriological Techniques / Q000379:methods*; D002213:Capsid / Q000378:metabolism*; D036022:Capsid Proteins / Q000235:genetics / Q000378:metabolism*; D005453:Fluorescence; D009160:Mycobacteriophages / Q000502:physiology*; D009161:Mycobacterium / Q000302:isolation & purification* / Q000821:virology; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D013194:Staining and Labeling / Q000379:methods*; D019065:Virus Assembly
chemical_list: D036022:Capsid Proteins; D011993:Recombinant Fusion Proteins