title: Micelle-mediated extraction of dibenzocyclooctadiene lignans from Schisandra chinensis with analysis by high-performance liquid chromatography.
abstract: Micelle-mediated extraction offers a convenient alternative to conventional extraction systems. A new method based on micelle-mediated extraction was developed for the separation and determination of dibenzocyclooctadiene lignans from Schisandra chinensis by high-performance liquid chromatography with photodiode array detection. Various experimental conditions using the micelle-mediated method were investigated to evaluate the extraction process. Ethylene glycol monoalkyl ether (Genapol X-080), a non-ionic surfactant oligoethylene glycol monoalkyl ether, was chosen as the extract solvent. The chromatographic separation was accomplished on a Shiseido Capcell Pak C18 analytical column (250 × 4.6mm i.d., 5 µm particle diameter), detected by ultraviolet absorption at 254 nm. The isocratic elution was achieved with a mobile phase composed of water-acetonitrile-formic acid (70:30:0.1) at a flow rate of 0.6 mL/min. The method was optimized and fully validated against dibenzocyclooctadiene lignans (schizandrin, gomisin A and gomisin N). With 15% Genapol X-080, a liquid to solid ratio of 100:1 (mL/g) and ultrasonic-assisted extraction for 60 min, the extraction percentage of total dibenzocyclooctadiene lignans reached the highest value. The non-ionic surfactant Genapol X-080 solution is an effective alternative for the extraction of bioactive lignans from S. chinensis.
authors.affiliation: College of Pharmacy and Research Institute of Pharmaceutical Science, Seoul National University, Seoul 151-742, Korea College of Pharmacy, Korea University, Sejong City 339-700, Korea.
authors.forename: Ki Yong
authors.initials: KY
authors.lastname: Lee
authors.affiliation: Institute for Life Science, Elcom Science Co. Ltd., Seoul 152-742, Korea.
authors.forename: Yong-Jun
authors.initials: YJ
authors.lastname: Shin
authors.affiliation: Institute for Life Science, Elcom Science Co. Ltd., Seoul 152-742, Korea.
authors.forename: Dae Hyun
authors.initials: DH
authors.lastname: Kim
authors.affiliation: Institute for Life Science, Elcom Science Co. Ltd., Seoul 152-742, Korea.
authors.forename: Jin-Ho
authors.initials: JH
authors.lastname: Park
authors.affiliation: College of Pharmacy, Yonsei University, Incheon 406-840, Korea.
authors.forename: Seung Hyun
authors.initials: SH
authors.lastname: Kim
authors.affiliation: Department of Pharmaceutical Analysis, College of Pharmacy, Chung-Ang University, Seoul 156-756, Korea.
authors.forename: Sang Beom
authors.initials: SB
authors.lastname: Han
authors.affiliation: College of Pharmacy and Research Institute of Pharmaceutical Science, Seoul National University, Seoul 151-742, Korea shsung@snu.ac.kr.
authors.forename: Sang Hyun
authors.initials: SH
authors.lastname: Sung
mesh_terms: D005591:Chemical Fractionation; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D034242:Cyclooctanes / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*; D017705:Lignans / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*; D008823:Micelles; D011092:Polyethylene Glycols / Q000737:chemistry; D015203:Reproducibility of Results; D032141:Schisandra / Q000737:chemistry*
chemical_list: D034242:Cyclooctanes; D017705:Lignans; D008823:Micelles; C510994:dibenzocyclooctadiene lignan; D011092:Polyethylene Glycols; C116996:genapol X 080