title: Aberrant expression of thePHF14gene in biliary tract cancer cells.
abstract: DNA copy number aberrations in human biliary tract cancer (BTC) cell lines were investigated using a high-density oligonucleotide microarray. A novel homozygous deletion was detected at chromosomal region 7p21.3 in the OZ cell line. Further validation experiments using genomic PCR revealed a homozygous deletion of a single gene, plant homeodomain (PHD) finger protein 14 (PHF14). NoPHF14mRNA or protein expression was detected, thus demonstrating the absence ofPHF14expression in the OZ cell line. Although the PHD finger protein is considered to be involved in chromatin-mediated transcriptional regulation, little is known about the function ofPHF14in cancer. The present study observed that the knock down ofPHF14using small interfering RNA (siRNA) enhanced the growth of the BTC cells. These observations suggest that aberrantPHF14expression may have a role in the tumorigenesis of BTC.
authors.affiliation: Department of Molecular Gastroenterology and Hepatology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto 602-8566, Japan.
authors.forename: Takako
authors.initials: T
authors.lastname: Akazawa
authors.forename: Kohichiroh
authors.initials: K
authors.lastname: Yasui
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Gen
authors.forename: Nobuhisa
authors.initials: N
authors.lastname: Yamada
authors.forename: Akira
authors.initials: A
authors.lastname: Tomie
authors.forename: Osamu
authors.initials: O
authors.lastname: Dohi
authors.forename: Hironori
authors.initials: H
authors.lastname: Mitsuyoshi
authors.forename: Nobuaki
authors.initials: N
authors.lastname: Yagi
authors.forename: Yoshito
authors.initials: Y
authors.lastname: Itoh
authors.forename: Yuji
authors.initials: Y
authors.lastname: Naito
authors.forename: Toshikazu
authors.initials: T
authors.lastname: Yoshikawa
keywords: PHF14; biliary tract cancer; deletion