title: HIV-1 viral protein R downregulates Ebp1 and stabilizes p53 in glioblastoma U87MG cells.
abstract: OBJECTIVE
  HIV-1 viral protein R (Vpr) inhibits cell growth and induces apoptosis in a wide range of cancers. However, the mechanism by which Vpr induces cell cycle arrest and apoptosis in GBM cell lines is unclear. The present work was taken to detect the proteins interacted with Vpr in U87MG cells.
  
  
  METHODS
  We analyzed the differential expression of proteins between glioblastoma cell U87MG treated with Ad-Vpr and untreated by 2-DE. We used antibody array analysis to analyze the common molecules in the apoptosis of U87MG induced by Vpr.
  
  
  RESULTS
  We analyzed the differential expression of proteins between U87MG cell treated with Ad-Vpr and untreated, and found that proteins related to DNA damage repair or different apoptosis pathways were involved in the G2 arrest and apoptosis mediated by Vpr. In addition, proliferation-associated protein 2G4 (PA2G4), also known as Ebp1, was down-regulated and p53 was up-regulated in U87MG cells treated with Ad-Vpr.
  
  
  CONCLUSIONS
  Our data suggest that Vpr may inhibit Ebp1 to stabilize p53, which in turn leads to G2 arrest and apoptosis in U87MG cells.
authors.affiliation: Tianjin Neurosurgical Institute, Tianjin Huanhu Hospital, Tianjin, 300060, People's Republic of China.
authors.forename: S
authors.initials: S
authors.lastname: Zhang
authors.forename: B
authors.initials: B
authors.lastname: Zhang
authors.forename: X
authors.initials: X
authors.lastname: Xu
authors.forename: L
authors.initials: L
authors.lastname: Wang
authors.forename: X
authors.initials: X
authors.lastname: Feng
authors.forename: Q
authors.initials: Q
authors.lastname: Wang
authors.forename: H
authors.initials: H
authors.lastname: Huang
authors.forename: J
authors.initials: J
authors.lastname: Wu
authors.forename: P
authors.initials: P
authors.lastname: Li
authors.forename: J
authors.initials: J
authors.lastname: Wang
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000378:metabolism*; D017209:Apoptosis / Q000502:physiology*; D015153:Blotting, Western; D001932:Brain Neoplasms / Q000378:metabolism / Q000821:virology*; D045744:Cell Line, Tumor; D015180:Electrophoresis, Gel, Two-Dimensional; D005909:Glioblastoma / Q000378:metabolism / Q000821:virology*; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis; D016601:RNA-Binding Proteins / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism*; D054325:vpr Gene Products, Human Immunodeficiency Virus
chemical_list: D048868:Adaptor Proteins, Signal Transducing; C101633:PA2G4 protein, human; D016601:RNA-Binding Proteins; C495901:TP53 protein, human; D016159:Tumor Suppressor Protein p53; D054325:vpr Gene Products, Human Immunodeficiency Virus; C514912:vpr protein, Human immunodeficiency virus 1