title: Molecular hydrogen regulates the expression of miR-9, miR-21 and miR-199 in LPS-activated retinal microglia cells.
abstract: OBJECTIVE
  To explore the potential mechanism of molecular hydrogen in the regulation of miRNA expression and signal-modulating activities.
  
  
  METHODS
  Retinal microglia cells were activated by Lipopolysaccharides (LPS) and then treated with hydrogen-saturated medium or normal medium without hydrogen. qRT-PCR was used to detect the expression difference in miR-9, miR-21 and miR-199 between these two groups. Moreover, the expression of LPS-induced signaling proteins, including Myd88, IKK-β, NF-κB, and PDCD4, were detected by Western blotting.
  
  
  RESULTS
  The results demonstrated a marked down-regulation of miR-9 and miR-21 and up-regulation of miR-199 by hydrogen treatment; the expression of Myd88 and IKK-β was decreased after hydrogen treatment, whereas PDCD4 was increased, and there was no significant change in NF-κB expression.
  
  
  CONCLUSIONS
  The results in the present study indicate that miR-9, miR-199 and miR-21 play an important role in the anti-inflammatory regulation of LPS-activated microglia cells by molecular hydrogen, which will help to explain the protective mechanism of molecular hydrogen against inflammatory injury.
authors.affiliation: Department of Ophthalmology, the Fourth Affiliated Hospital of Harbin Medical University, Harbin 150001, Heilongjiang Province, China.
authors.forename: Guo-Dan
authors.initials: GD
authors.lastname: Liu
authors.forename: Hong
authors.initials: H
authors.lastname: Zhang
authors.forename: Lin
authors.initials: L
authors.lastname: Wang
authors.forename: Qing
authors.initials: Q
authors.lastname: Han
authors.forename: Shi-Feng
authors.initials: SF
authors.lastname: Zhou
authors.forename: Ping
authors.initials: P
authors.lastname: Liu
keywords: Lipopolysaccharides; Toll-like receptor 4; hydrogen; miR-199; miR-21; miR-9