title: Molecular characterization, alternative splicing and expression analysis of bovine DBC1.
abstract: Deleted in breast cancer 1 (DBC1, KIAA1967, p30 DBC) is a novel protein that has been recently shown to bind and regulate SIRT1. Loss of function of DBC1 increased SIRT1 deacetylase activity, which promotes "browning" of WAT by deacetylating peroxisome proliferator-activated receptor (PPARγ) on Lys268 and Lys293. In the present study, we have cloned and characterized the bovine DBC1 gene. Two transcript variants of bovine DBC1 were identified, designated DBC1-A and DBC1-B, respectively, which were both located in nucleus. Protein sequence analysis showed that DBC1-A was well conserved across species. Expression analysis of DBC1 in seven different tissues of calves and bulls by RT-PCR indicated that the two transcripts were ubiquitously expressed. However, the relatively level of DBC1-A was higher when compared to DBC1-B in all examined tissues. Surprisingly, the expression of DBC1-A was extraordinary high in calves adipose tissue, which implicated its potential key role in regulating calve adipocyte development. These findings provide new insight into our understanding of the biochemical characteristics and physiological role of bovine DBC1.
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling, Shaanxi 712100, China. limingxun@live.com
authors.forename: Mingxun
authors.initials: M
authors.lastname: Li
authors.forename: Xiaomei
authors.initials: X
authors.lastname: Sun
authors.forename: Liushuai
authors.initials: L
authors.lastname: Hua
authors.forename: Yongzhen
authors.initials: Y
authors.lastname: Huang
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.forename: Xiukai
authors.initials: X
authors.lastname: Cao
authors.forename: Zhaoyang
authors.initials: Z
authors.lastname: Zhan
authors.forename: Xianyong
authors.initials: X
authors.lastname: Lan
authors.forename: Hong
authors.initials: H
authors.lastname: Chen
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics*; D017398:Alternative Splicing; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D017931:DNA Primers; D010802:Phylogeny; D012333:RNA, Messenger / Q000235:genetics; D013347:Subcellular Fractions / Q000378:metabolism
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D017931:DNA Primers; D012333:RNA, Messenger
keywords: AS; Alternative splicing; Bovine; DBC1; DBC1 gene; Expression profiles; ORF; SIRT1; Subcellular distribution; alternative splicing; deleted in breast cancer 1; open reading frame; silent information regulator 1