title: Role of the postnatal radial glial scaffold for the development of the dentate gyrus as revealed by Reelin signaling mutant mice.
abstract: During dentate gyrus development, the early embryonic radial glial scaffold is replaced by a secondary glial scaffold around birth. In contrast to neocortical and early dentate gyrus radial glial cells, these postnatal glial cells are severely altered with regard to position and morphology in reeler mice lacking the secreted protein Reelin. In this study, we focus on the functional impact of these defects. Most radial glial cells throughout the nervous system serve as scaffolds for migrating neurons and precursor cells for both neurogenesis and gliogenesis. Precursor cell function has been demonstrated for secondary radial glial cells but the exact function of these late glial cells in granule cell migration and positioning is not clear. No data exist concerning the interplay between granule neurons and late radial glial cells during dentate gyrus development. Herein, we show that despite the severe morphological defects in the reeler dentate gyrus, the precursor function of secondary radial glial cells is not impaired during development in reeler mice. In addition, selective ablation of Disabled-1, an intracellular adaptor protein essential for Reelin signaling, in neurons but not in glial cells allowed us to distinguish effects of Reelin signaling on radial glial cells from possible secondary effects based on defective granule cells positioning.
authors.affiliation: Institute for Structural Neurobiology, Center for Molecular Neurobiology Hamburg, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.
authors.forename: Bianka
authors.initials: B
authors.lastname: Brunne
authors.forename: Santos
authors.initials: S
authors.lastname: Franco
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Bouché
authors.forename: Joachim
authors.initials: J
authors.lastname: Herz
authors.forename: Brian W
authors.initials: BW
authors.lastname: Howell
authors.forename: Jasmine
authors.initials: J
authors.lastname: Pahle
authors.forename: Ulrich
authors.initials: U
authors.lastname: Müller
authors.forename: Petra
authors.initials: P
authors.lastname: May
authors.forename: Michael
authors.initials: M
authors.lastname: Frotscher
authors.forename: Hans H
authors.initials: HH
authors.lastname: Bock
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D015816:Cell Adhesion Molecules, Neuronal / Q000096:biosynthesis / Q000172:deficiency*; D002478:Cells, Cultured; D018891:Dentate Gyrus / Q000254:growth & development / Q000378:metabolism*; D063928:Ependymoglial Cells / Q000502:physiology*; D016326:Extracellular Matrix Proteins / Q000096:biosynthesis / Q000172:deficiency*; D051379:Mice; D057507:Mice, 129 Strain; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008818:Mice, Neurologic Mutants; D009154:Mutation; D009419:Nerve Tissue Proteins / Q000096:biosynthesis / Q000172:deficiency* / Q000235:genetics; D010641:Phenotype; D011994:Recombinant Proteins / Q000096:biosynthesis; D000091042:Reelin Protein; D012697:Serine Endopeptidases / Q000096:biosynthesis / Q000172:deficiency*; D015398:Signal Transduction / Q000235:genetics*; D013234:Stem Cells / Q000378:metabolism
chemical_list: D015816:Cell Adhesion Molecules, Neuronal; C103773:Dab1 protein, mouse; D016326:Extracellular Matrix Proteins; D009419:Nerve Tissue Proteins; D011994:Recombinant Proteins; D000091042:Reelin Protein; C000717128:Reln protein, mouse; D012697:Serine Endopeptidases
keywords: development; differentiation; gliogenesis; hippocampus; reeler