title: Investigation of protein expression profiles of erythritol-producing Candida magnoliae in response to glucose perturbation.
abstract: Protein expression patterns of an erythritol-producing yeast, Candida magnoliae, were analyzed to identify differentially expressed proteins in response to glucose perturbation. Specifically, wild type C. magnoliae was grown under high and low glucose conditions and the cells were harvested at both mid-exponential and erythritol production phases for proteomic studies. In order to analyze intracellular protein abundances from the harvested cells quantitatively, total intracellular proteins were extracted and applied to two-dimensional gel electrophoresis for separation and visualization of individual proteins. Among the proteins distributed in the range of pI 4-7 and molecular weight 29-97kDa, five osmo-responsive proteins were drastically changed in response to glucose perturbation. Hsp60 (Heat-shock protein 60), transaldolase and NADH:quinone oxidoreductase were down-regulated under the high glucose condition and Bro1 (BCK1-like Resistance to Osmotic shock) and Eno1 (enolase1) were up-regulated. These proteins are directly or indirectly related with cellular stress response. Importantly, protein expression patterns of Hsp60, Bro1 and Eno1 were strongly correlated with previous studies identifying the proteins perturbed by osmotic stress for other organisms including Saccharomyces cerevisiae.
authors.affiliation: Department of Food Science and Human Nutrition, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.
authors.forename: Hyo Jin
authors.initials: HJ
authors.lastname: Kim
authors.forename: Hyeong-Rho
authors.initials: HR
authors.lastname: Lee
authors.forename: Chang Sup
authors.initials: CS
authors.lastname: Kim
authors.forename: Yong-Su
authors.initials: YS
authors.lastname: Jin
authors.forename: Jin-Ho
authors.initials: JH
authors.lastname: Seo
mesh_terms: D001709:Biotechnology; D002175:Candida / Q000254:growth & development / Q000378:metabolism*; D018834:Chaperonin 60 / Q000378:metabolism; D042967:Electron Transport Complex I / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D004896:Erythritol / Q000096:biosynthesis*; D005285:Fermentation; D005656:Fungal Proteins / Q000302:isolation & purification / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism*; D008954:Models, Biological; D009997:Osmotic Pressure; D010751:Phosphopyruvate Hydratase / Q000378:metabolism; D040901:Proteomics; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry; D014153:Transaldolase / Q000378:metabolism
chemical_list: D018834:Chaperonin 60; D005656:Fungal Proteins; D014153:Transaldolase; D010751:Phosphopyruvate Hydratase; D042967:Electron Transport Complex I; D005947:Glucose; D004896:Erythritol
keywords: Candida magnoliae; Erythritol; Osmo-responsive protein; Proteomics