title: Functionalization of 3D scaffolds with protein-releasing biomaterials for intracellular delivery.
abstract: Appropriate combinations of mechanical and biological stimuli are required to promote proper colonization of substrate materials in regenerative medicine. In this context, 3D scaffolds formed by compatible and biodegradable materials are under continuous development in an attempt to mimic the extracellular environment of mammalian cells. We have here explored how novel 3D porous scaffolds constructed by polylactic acid, polycaprolactone or chitosan can be decorated with bacterial inclusion bodies, submicron protein particles formed by releasable functional proteins. A simple dipping-based decoration method tested here specifically favors the penetration of the functional particles deeper than 300μm from the materials' surface. The functionalized surfaces support the intracellular delivery of biologically active proteins to up to more than 80% of the colonizing cells, a process that is slightly influenced by the chemical nature of the scaffold. The combination of 3D soft scaffolds and protein-based sustained release systems (Bioscaffolds) offers promise in the fabrication of bio-inspired hybrid matrices for multifactorial control of cell proliferation in tissue engineering under complex architectonic setting-ups.
authors.affiliation: Institut de Biotecnologia i de Biomedicina, Universitat Autònoma de Barcelona, Bellaterra, 08193 Barcelona, Spain.
authors.forename: Joaquin
authors.initials: J
authors.lastname: Seras-Franzoso
authors.forename: Christoph
authors.initials: C
authors.lastname: Steurer
authors.forename: Mònica
authors.initials: M
authors.lastname: Roldán
authors.forename: Meritxell
authors.initials: M
authors.lastname: Vendrell
authors.forename: Carla
authors.initials: C
authors.lastname: Vidaurre-Agut
authors.forename: Anna
authors.initials: A
authors.lastname: Tarruella
authors.forename: Laura
authors.initials: L
authors.lastname: Saldaña
authors.forename: Nuria
authors.initials: N
authors.lastname: Vilaboa
authors.forename: Marc
authors.initials: M
authors.lastname: Parera
authors.forename: Elisa
authors.initials: E
authors.lastname: Elizondo
authors.forename: Imma
authors.initials: I
authors.lastname: Ratera
authors.forename: Nora
authors.initials: N
authors.lastname: Ventosa
authors.forename: Jaume
authors.initials: J
authors.lastname: Veciana
authors.forename: Alberto J
authors.initials: AJ
authors.lastname: Campillo-Fernández
authors.forename: Elena
authors.initials: E
authors.lastname: García-Fruitós
authors.forename: Esther
authors.initials: E
authors.lastname: Vázquez
authors.forename: Antonio
authors.initials: A
authors.lastname: Villaverde
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000008:administration & dosage* / Q000737:chemistry; D001672:Biocompatible Materials / Q000737:chemistry; D002478:Cells, Cultured; D048271:Chitosan / Q000737:chemistry; D004926:Escherichia coli; D005347:Fibroblasts; D006367:HeLa Cells; D006801:Humans; D002479:Inclusion Bodies / Q000737:chemistry*; D019344:Lactic Acid / Q000737:chemistry; D051379:Mice; D041681:NIH 3T3 Cells; D011091:Polyesters / Q000737:chemistry; D011108:Polymers / Q000737:chemistry; D054457:Tissue Scaffolds
chemical_list: D001426:Bacterial Proteins; D001672:Biocompatible Materials; D011091:Polyesters; D011108:Polymers; C016240:polycaprolactone; D019344:Lactic Acid; C033616:poly(lactide); D048271:Chitosan
keywords: 3D scaffolds; Bioscaffold; Bottom-up delivery; Nanoparticles; Polylactic acid (PLA); Tissue engineering