title: Mapping the functional yeast ABC transporter interactome.
abstract: ATP-binding cassette (ABC) transporters are a ubiquitous class of integral membrane proteins of immense clinical interest because of their strong association with human disease and pharmacology. To improve our understanding of these proteins, we used membrane yeast two-hybrid technology to map the protein interactome of all of the nonmitochondrial ABC transporters in the model organism Saccharomyces cerevisiae and combined this data with previously reported yeast ABC transporter interactions in the BioGRID database to generate a comprehensive, integrated 'interactome'. We show that ABC transporters physically associate with proteins involved in an unexpectedly diverse range of functions. We specifically examine the importance of the physical interactions of ABC transporters in both the regulation of one another and in the modulation of proteins involved in zinc homeostasis. The interaction network presented here will be a powerful resource for increasing our fundamental understanding of the cellular role and regulation of ABC transporters.
authors.affiliation: Donnelly Centre, University of Toronto, Toronto, Ontario, Canada.
authors.forename: Jamie
authors.initials: J
authors.lastname: Snider
authors.forename: Asad
authors.initials: A
authors.lastname: Hanif
authors.forename: Mid Eum
authors.initials: ME
authors.lastname: Lee
authors.forename: Ke
authors.initials: K
authors.lastname: Jin
authors.forename: Analyn R
authors.initials: AR
authors.lastname: Yu
authors.forename: Chris
authors.initials: C
authors.lastname: Graham
authors.forename: Matthew
authors.initials: M
authors.lastname: Chuk
authors.forename: Dunja
authors.initials: D
authors.lastname: Damjanovic
authors.forename: Marta
authors.initials: M
authors.lastname: Wierzbicka
authors.forename: Priscilla
authors.initials: P
authors.lastname: Tang
authors.forename: Dina
authors.initials: D
authors.lastname: Balderes
authors.forename: Victoria
authors.initials: V
authors.lastname: Wong
authors.forename: Matthew
authors.initials: M
authors.lastname: Jessulat
authors.forename: Katelyn D
authors.initials: KD
authors.lastname: Darowski
authors.forename: Bryan-Joseph
authors.initials: BJ
authors.lastname: San Luis
authors.forename: Igor
authors.initials: I
authors.lastname: Shevelev
authors.forename: Stephen L
authors.initials: SL
authors.lastname: Sturley
authors.forename: Charles
authors.initials: C
authors.lastname: Boone
authors.forename: Jack F
authors.initials: JF
authors.lastname: Greenblatt
authors.forename: Zhaolei
authors.initials: Z
authors.lastname: Zhang
authors.forename: Christian M
authors.initials: CM
authors.lastname: Paumi
authors.forename: Mohan
authors.initials: M
authors.lastname: Babu
authors.forename: Hay-Oak
authors.initials: HO
authors.lastname: Park
authors.forename: Susan
authors.initials: S
authors.lastname: Michaelis
authors.forename: Igor
authors.initials: I
authors.lastname: Stagljar
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000378:metabolism*; D011485:Protein Binding; D025941:Protein Interaction Mapping; D012441:Saccharomyces cerevisiae / Q000737:chemistry / Q000378:metabolism*; D020798:Two-Hybrid System Techniques
chemical_list: D018528:ATP-Binding Cassette Transporters