title: Evaluation of the mutagenic effect of the iodinated contrast medium Urografina® 292 using the micronucleus test in mouse bone marrow cells.
abstract: Contrast media (CM) are frequently used in diagnostic radiology and in radiotherapy as a diagnostic tool and in treatment planning. Previous studies have demonstrated that these compounds induce chromosomal aberrations. This study evaluates the mutagenic effects induced by the contrast medium Urografina® 292 (meglumine amidotrizoate and sodium-ionic dimmer) in bone marrow cells (BMC) of mice in vivo. Micronuclei assay was performed in BMC of CF-1 mice injected with CM 1.5 and 3.0 mL/kg intravenous doses and 1.0, 2.0, 3.0 mL/kg intraperitoneal doses. The animals were beheaded 24 h after treatment by cervical dislocation, and femur BMC from each animal were used in the micronucleus test. The group treated with the highest intravenous injection of Urografina® 292 (3.0 mL/kg) presented an increase in the frequency of micronucleated polychromatic erythrocytes (MNPCEs) in relation at the control group (P<0.05). The results obtained after intraperitoneal administration of CM showed that all doses (1.0 mL/kg, 2.0 mL/kg and 3.0 mL/kg) increased the frequency of MNPCEs, being significantly different from the negative control (P< 0.01). The present results suggest that iodinated contrast media Urografina® 292 may cause a significant increase of cytogenetic damage in bone marrow cells of mice.
authors.affiliation: Programa de Pós-Graduação em Ciências da Saúde, Unidade Acadêmica de Ciências da Saúde, Universidade do Extremo Sul Catarinense/UNESC, Bairro Universitário, Criciúma, SC, Brasil.
authors.forename: Mônica B B
authors.initials: MB
authors.lastname: Belle
authors.forename: Daniela D
authors.initials: DD
authors.lastname: Leffa
authors.forename: Daliane
authors.initials: D
authors.lastname: Mazzorana
authors.forename: Vanessa M
authors.initials: VM
authors.lastname: De Andrade
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000187:drug effects*; D002869:Chromosome Aberrations / Q000139:chemically induced*; D003287:Contrast Media / Q000008:administration & dosage / Q000633:toxicity*; D003974:Diatrizoate Meglumine / Q000008:administration & dosage / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D015162:Micronucleus Tests / Q000379:methods
chemical_list: D003287:Contrast Media; D003974:Diatrizoate Meglumine