title: BtpB, a novel Brucella TIR-containing effector protein with immune modulatory functions.
abstract: Several bacterial pathogens have TIR domain-containing proteins that contribute to their pathogenesis. We identified a second TIR-containing protein in Brucella spp. that we have designated BtpB. We show it is a potent inhibitor of TLR signaling, probably via MyD88. BtpB is a novel Brucella effector that is translocated into host cells and interferes with activation of dendritic cells. In vivo mouse studies revealed that BtpB is contributing to virulence and control of local inflammatory responses with relevance in the establishment of chronic brucellosis. Together, our results show that BtpB is a novel Brucella effector that plays a major role in the modulation of host innate immune response during infection.
authors.affiliation: Aix-Marseille Univ UM 2, Centre d'Immunologie de Marseille-Luminy Marseille, France.
authors.forename: Suzana P
authors.initials: SP
authors.lastname: Salcedo
authors.forename: María I
authors.initials: MI
authors.lastname: Marchesini
authors.forename: Clara
authors.initials: C
authors.lastname: Degos
authors.forename: Matthieu
authors.initials: M
authors.lastname: Terwagne
authors.forename: Kristine
authors.initials: K
authors.lastname: Von Bargen
authors.forename: Hubert
authors.initials: H
authors.lastname: Lepidi
authors.forename: Claudia K
authors.initials: CK
authors.lastname: Herrmann
authors.forename: Thais L
authors.initials: TL
authors.lastname: Santos Lacerda
authors.forename: Paul R C
authors.initials: PR
authors.lastname: Imbert
authors.forename: Philippe
authors.initials: P
authors.lastname: Pierre
authors.forename: Lena
authors.initials: L
authors.lastname: Alexopoulou
authors.forename: Jean-Jacques
authors.initials: JJ
authors.lastname: Letesson
authors.forename: Diego J
authors.initials: DJ
authors.lastname: Comerci
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Gorvel
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001426:Bacterial Proteins / Q000276:immunology / Q000378:metabolism*; D002002:Brucella / Q000276:immunology* / Q000472:pathogenicity*; D002006:Brucellosis / Q000276:immunology / Q000382:microbiology; D003713:Dendritic Cells / Q000276:immunology; D004195:Disease Models, Animal; D005260:Female; D057131:Immune Evasion; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D015398:Signal Transduction; D016019:Survival Analysis; D051193:Toll-Like Receptors / Q000276:immunology; D037521:Virulence Factors / Q000276:immunology / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D051193:Toll-Like Receptors; D037521:Virulence Factors
keywords: Brucella; Btp1/BtpA; DC; NF-κB; TIR domain; TLR