title: Modelling blood flow and metabolism in the piglet brain during hypoxia-ischaemia: simulating brain energetics.
abstract: We have developed a computational model to simulate hypoxia-ischaemia (HI) in the neonatal piglet brain. It has been extended from a previous model by adding the simulation of carotid artery occlusion and including pH changes in the cytoplasm. Here, simulations from the model are compared with near-infrared spectroscopy (NIRS) and phosphorus magnetic resonance spectroscopy (MRS) measurements from two piglets during HI and short-term recovery. One of these piglets showed incomplete recovery after HI, and this is modelled by considering some of the cells to be dead. This is consistent with the results from MRS and the redox state of cytochrome-c-oxidase as measured by NIRS. However, the simulations do not match the NIRS haemoglobin measurements. The model therefore predicts that further physiological changes must also be taking place if the hypothesis of dead cells is correct.
authors.affiliation: CoMPLEX, University College London, London, UK. t.moroz@ucl.ac.uk.
authors.forename: Tracy
authors.initials: T
authors.lastname: Moroz
authors.affiliation: CoMPLEX, University College London, London, UK.
authors.forename: Tharindi
authors.initials: T
authors.lastname: Hapuarachchi
authors.affiliation: Medical Physics and Bioengineering, University College London Hospitals, London, UK.
authors.forename: Alan
authors.initials: A
authors.lastname: Bainbridge
authors.affiliation: Medical Physics and Bioengineering, University College London Hospitals, London, UK.
authors.forename: David
authors.initials: D
authors.lastname: Price
authors.affiliation: Medical Physics and Bioengineering, University College London Hospitals, London, UK.
authors.forename: Ernest
authors.initials: E
authors.lastname: Cady
authors.affiliation: Department of Medical Physics and Bioengineering, University College London, London, UK.
authors.forename: Ether
authors.initials: E
authors.lastname: Baer
authors.affiliation: Institute for Women's Health, University College London, London, UK.
authors.forename: Kevin
authors.initials: K
authors.lastname: Broad
authors.affiliation: Institute for Women's Health, University College London, London, UK.
authors.forename: Mojgan
authors.initials: M
authors.lastname: Ezzati
authors.affiliation: Institute of Neurology, University College London, London, UK.
authors.forename: David
authors.initials: D
authors.lastname: Thomas
authors.affiliation: Institute of Neurology, University College London, London, UK.
authors.forename: Xavier
authors.initials: X
authors.lastname: Golay
authors.affiliation: Institute for Women's Health, University College London, London, UK.
authors.forename: Nicola J
authors.initials: NJ
authors.lastname: Robertson
authors.affiliation: School of Biological Sciences, University of Essex, Colchester, UK.
authors.forename: Chris E
authors.initials: CE
authors.lastname: Cooper
authors.affiliation: Department of Medical Physics and Bioengineering, University College London, London, UK.
authors.forename: Ilias
authors.initials: I
authors.lastname: Tachtsidis
mesh_terms: D000818:Animals; D000831:Animals, Newborn / Q000378:metabolism / Q000502:physiology; D001921:Brain / Q000098:blood supply* / Q000378:metabolism*; D002339:Carotid Arteries / Q000378:metabolism / Q000503:physiopathology; D002560:Cerebrovascular Circulation; D004195:Disease Models, Animal; D003576:Electron Transport Complex IV / Q000378:metabolism; D004734:Energy Metabolism; D006454:Hemoglobins / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D020925:Hypoxia-Ischemia, Brain / Q000097:blood / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D010084:Oxidation-Reduction; D010101:Oxygen Consumption / Q000502:physiology; D019265:Spectroscopy, Near-Infrared / Q000379:methods; D013552:Swine
chemical_list: D006454:Hemoglobins; D003576:Electron Transport Complex IV