title: Catastrophic nuclear envelope collapse in cancer cell micronuclei.
abstract: During mitotic exit, missegregated chromosomes can recruit their own nuclear envelope (NE) to form micronuclei (MN). MN have reduced functioning compared to primary nuclei in the same cell, although the two compartments appear to be structurally comparable. Here we show that over 60% of MN undergo an irreversible loss of compartmentalization during interphase due to NE collapse. This disruption of the MN, which is induced by defects in nuclear lamina assembly, drastically reduces nuclear functions and can trigger massive DNA damage. MN disruption is associated with chromatin compaction and invasion of endoplasmic reticulum (ER) tubules into the chromatin. We identified disrupted MN in both major subtypes of human non-small-cell lung cancer, suggesting that disrupted MN could be a useful objective biomarker for genomic instability in solid tumors. Our study shows that NE collapse is a key event underlying MN dysfunction and establishes a link between aberrant NE organization and aneuploidy.
authors.affiliation: Molecular and Cell Biology Laboratory, Salk Institute for Biological Studies, 10010 N. Torrey Pines Road, La Jolla, CA 92037, USA.
authors.forename: Emily M
authors.initials: EM
authors.lastname: Hatch
authors.forename: Andrew H
authors.initials: AH
authors.lastname: Fischer
authors.forename: Thomas J
authors.initials: TJ
authors.lastname: Deerinck
authors.forename: Martin W
authors.initials: MW
authors.lastname: Hetzer
mesh_terms: D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics / Q000473:pathology*; D045744:Cell Line, Tumor; D002467:Cell Nucleus / Q000378:metabolism; D004249:DNA Damage; D042822:Genomic Instability; D006801:Humans; D007399:Interphase; D034882:Lamins / Q000378:metabolism; D008175:Lung Neoplasms / Q000235:genetics / Q000473:pathology*; D048629:Micronuclei, Chromosome-Defective; D009685:Nuclear Envelope / Q000378:metabolism*
chemical_list: D034882:Lamins