title: Retrotransposon insertional polymorphism in Iranian bread wheat cultivars and breeding lines revealed by IRAP and REMAP markers.
abstract: Inter-retrotransposon amplified polymorphisms (IRAPs) and retrotransposon-microsatellite amplified polymorphisms (REMAPs) were used to detect retrotransposon integration events and genetic diversity in 101 Iranian bread wheat (Triticum aestivum L.) cultivars and breeding lines. The 9 IRAP primers amplified 128 loci, and 20 REMAP primers amplified 263 loci. Percentage of polymorphic loci, average expected heterozygosity, number of effective alleles, and Shannon's information index for the REMAP markers were slightly higher than those for the IRAP markers. The same estimated parameters calculated for native and nonnative retrotransposons were not considerably different. A Mantel test between IRAP and REMAP cophenetic matrices evidenced no significant correlation. Cluster analysis based on the Dice similarity coefficient and complete linkage algorithm using IRAP+REMAP loci identified five groups among the genotypes studied that could be applied as crossing parents in T. aestivum breeding programs.
authors.affiliation: Department of Plant Breeding and Biotechnology, Faculty of Agriculture, Urmia University, Urmia, Iran.
authors.forename: Shilan
authors.initials: S
authors.lastname: Nasri
authors.forename: Babak
authors.initials: B
authors.lastname: Abdollahi Mandoulakani
authors.forename: Reza
authors.initials: R
authors.lastname: Darvishzadeh
authors.forename: Iraj
authors.initials: I
authors.lastname: Bernousi
mesh_terms: D001939:Bread; D001947:Breeding; D016000:Cluster Analysis; D017931:DNA Primers; D008040:Genetic Linkage; D014644:Genetic Variation; D018745:Genome, Plant; D005838:Genotype; D007492:Iran; D018895:Microsatellite Repeats; D016254:Mutagenesis, Insertional; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D018626:Retroelements / Q000235:genetics*; D020079:Terminal Repeat Sequences; D014908:Triticum / Q000235:genetics*
chemical_list: D017931:DNA Primers; D018626:Retroelements