title: Cloning, expression, purification, crystallization and preliminary crystallographic analysis of the kinase domain of AtMAP4Kalpha2 from Arabidopsis thaliana.
abstract: Arabidopsis thaliana (At) MAP4Kalpha2, a member of the Ste20/PAK-like protein kinase family, is an essential component of the septum initiation network involved in cell division. To better understand the mode of action of AtMAP4Kalpha2, a structural biology approach has been pursued. In this study, the kinase domain of AtMAP4Kalpha2 was cloned, expressed, purified and crystallized. The crystals diffracted to 1.9 Å resolution and belonged to space group C2221, with unit-cell parameters a = 55.27, b = 82.93, c = 133.15 Å.
authors.affiliation: College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400716, People's Republic of China.
authors.forename: Liqiang
authors.initials: L
authors.lastname: Shen
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Du
authors.forename: Qing
authors.initials: Q
authors.lastname: Su
authors.forename: Mingxia
authors.initials: M
authors.lastname: Li
authors.forename: Zhiqin
authors.initials: Z
authors.lastname: Zhou
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000201:enzymology* / Q000235:genetics; D029681:Arabidopsis Proteins / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism*; D003001:Cloning, Molecular; D003460:Crystallization; D018360:Crystallography, X-Ray; D008969:Molecular Sequence Data; D017346:Protein Serine-Threonine Kinases / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism*; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D017386:Sequence Homology, Amino Acid
chemical_list: D029681:Arabidopsis Proteins; D011994:Recombinant Proteins; C583950:MAP4Kalpha2 protein, Arabidopsis; D017346:Protein Serine-Threonine Kinases
keywords: Arabidopsis thaliana; AtMAP4Kalpha2