title: Analysis of differentially expressed proteins in late-stationary growth phase of Mycobacterium tuberculosis H37Rv.
abstract: Mycobacterium tuberculosis is a causative agent of tuberculosis (TB). The ability of M. tuberculosis to be quiescent in the cell has caused the emergence of latent infection. A comprehensive proteomic analysis of M. tuberculosis H37Rv over three growth phases, namely mid-log (14-day culture), early stationary (28-day culture), and late stationary (50-day culture), was performed in order to study the change in proteome from the mid-log phase to late-stationary phase. Combination methods of two-dimensional electrophoresis (2-DE) and tandem mass spectrometry were used to generate proteome maps of M. tuberculosis at different growth phases. Ten proteins were detected differentially expressed in the late-stationary phase compared with the other two phases. These proteins were SucD, TrpD, and Rv2161c, which belong to metabolic pathway proteins; FadE5, AccD5, DesA1, and Rv1139c are proteins involved in cell wall or lipid biosynthesis, whereas TB21.7 and Rv3224 are conserved hypothetical proteins with unknown function. A surface antigen protein, DesA1, was not detectable in the late-stationary phase, although present in both log and early-stationary phases. The changes in the expression levels of these proteins were in line with the growth environment changes of the bacteria from mid-log phase to late-stationary phase. The information gathered may be valuable in the intervention against latent TB infection.
authors.affiliation: School of Pharmaceutical Sciences, University Sains Malaysia, Minden, Penang, Malaysia.
authors.forename: Kai-Cheen
authors.initials: KC
authors.lastname: Ang
authors.forename: Pazilah
authors.initials: P
authors.lastname: Ibrahim
authors.forename: Lay-Harn
authors.initials: LH
authors.lastname: Gam
mesh_terms: D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D009169:Mycobacterium tuberculosis / Q000235:genetics* / Q000254:growth & development; D014176:Protein Biosynthesis; D011499:Protein Processing, Post-Translational; D020543:Proteome / Q000235:genetics; D040901:Proteomics; D053719:Tandem Mass Spectrometry
chemical_list: D001426:Bacterial Proteins; D020543:Proteome
keywords: Mycobacterium tuberculosis; biomarker; differentially expressed proteins; expression proteomics