title: A novel statistical analysis and interpretation of flow cytometry data.
abstract: A recently developed class of models incorporating the cyton model of population generation structure into a conservation-based model of intracellular label dynamics is reviewed. Statistical aspects of the data collection process are quantified and incorporated into a parameter estimation scheme. This scheme is then applied to experimental data for PHA-stimulated CD4+T and CD8+T cells collected from two healthy donors. This novel mathematical and statistical framework is shown to form the basis for accurate, meaningful analysis of cellular behaviour for a population of cells labelled with the dye carboxyfluorescein succinimidyl ester and stimulated to divide.
authors.affiliation: Center for Research in Scientific Computation and Center for Quantitative Sciences in Biomedicine, North Carolina State University, Raleigh, NC 27695-8212, USA. htbanks@ncsu.edu
authors.forename: H T
authors.initials: HT
authors.lastname: Banks
authors.forename: D F
authors.initials: DF
authors.lastname: Kapraun
authors.forename: W Clayton
authors.initials: WC
authors.lastname: Thompson
authors.forename: Cristina
authors.initials: C
authors.lastname: Peligero
authors.forename: Jordi
authors.initials: J
authors.lastname: Argilaguet
authors.forename: Andreas
authors.initials: A
authors.lastname: Meyerhans
mesh_terms: D015496:CD4-Positive T-Lymphocytes / Q000187:drug effects / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000187:drug effects / Q000276:immunology; D049109:Cell Proliferation / Q000187:drug effects; D003627:Data Interpretation, Statistical; D005434:Flow Cytometry / Q000706:statistics & numerical data*; D005452:Fluoresceins; D005456:Fluorescent Dyes; D006801:Humans; D055641:Mathematical Concepts; D008934:Mitogens / Q000494:pharmacology; D018448:Models, Immunological; D015233:Models, Statistical; D010835:Phytohemagglutinins / Q000494:pharmacology; D013388:Succinimides
chemical_list: C087165:5-(6)-carboxyfluorescein diacetate succinimidyl ester; D005452:Fluoresceins; D005456:Fluorescent Dyes; D008934:Mitogens; D010835:Phytohemagglutinins; D013388:Succinimides