title: A review of reagents for fluorescence microscopy of cellular compartments and structures, part I: BacMam labeling and reagents for vesicular structures.
abstract: Fluorescent labeling of vesicular structures in cultured cells, particularly for live cells, can be challenging for a number of reasons. The first challenge is to identify a reagent that will be specific enough where some structures have a number of potential reagents and others very few options. The emergence of BacMam constructs has allowed more easy-to-use choices. Presented here is a discussion of BacMam constructs as well as a review of commercially-available reagents for labeling vesicular structures in cells, including endosomes, peroxisomes, lysosomes, and autophagosomes, complete with a featured reagent for each structure, recommended protocol, troubleshooting guide, and example image.
authors.affiliation: Molecular Probes Labeling and Detection, Life Technologies, Eugene, Oregon.
authors.forename: Nick J
authors.initials: NJ
authors.lastname: Dolman
authors.affiliation: Molecular Probes Labeling and Detection, Life Technologies, Eugene, Oregon.
authors.forename: Jason A
authors.initials: JA
authors.lastname: Kilgore
authors.affiliation: National High Magnetic Field Laboratory and Department of Biological Science, Florida State University, Tallahassee, Florida.
authors.forename: Michael W
authors.initials: MW
authors.lastname: Davidson
mesh_terms: D000818:Animals; D002451:Cell Compartmentation; D022162:Cytoplasmic Vesicles / Q000378:metabolism*; D006801:Humans; D007202:Indicators and Reagents; D008856:Microscopy, Fluorescence / Q000379:methods*; D013194:Staining and Labeling; D014161:Transduction, Genetic
chemical_list: D007202:Indicators and Reagents