title: IL-27 is elevated in acute lung injury and mediates inflammation.
abstract: Cytokines play a critical role in the development of acute lung injury (ALI)/acute respiratory distress syndrome (ARDS). Here we investigated whether IL-27 was elevated in patients with ALI/ARDS and its potential clinical significance. Bronchoalveolar lavage (BAL) and serum samples were obtained from 58 ALI/ARDS patients, and 25 control healthy volunteers. IL-27 and other inflammatory mediators were measured in BAL and serum by ELISA. Besides, a mouse model of cecal ligation and puncture (CLP)-induced lung inflammation/injury was established, and serum, BAL fluid and tissues were collected for analyses in the presence or absence of IL-27 neutralizing antibodies. BAL IL-27 was found to be significantly higher in patients with ALI/ARDS than that in controls, particularly of pulmonary origin; serum IL-27 was also significantly higher. Increased IL-27 was associated with markers of inflammation, and correlated with disease severity of patients in ALI/ARDS. In a mouse model of CLP-induced lung inflammation/injury, elevated IL-27 levels were observed in the lung, serum, and BAL fluids. IL-27 neutralizing antibody treatment reduced pulmonary inflammation and lung injury and improved mouse survival in response to CLP. Therefore, IL-27 is a critical cytokine in ALI/ARDS and inhibition of IL-27 may open a promising approach for ALI/ARDS patients.
authors.affiliation: Department of Emergency and Intensive Care Unit, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Xu
authors.forename: Qiong
authors.initials: Q
authors.lastname: Liu
authors.forename: Shihui
authors.initials: S
authors.lastname: Lin
authors.forename: Na
authors.initials: N
authors.lastname: Shen
authors.forename: Yibing
authors.initials: Y
authors.lastname: Yin
authors.forename: Ju
authors.initials: J
authors.lastname: Cao
mesh_terms: D055371:Acute Lung Injury / Q000276:immunology* / Q000628:therapy; D000328:Adult; D000818:Animals; D019138:Antibodies, Blocking / Q000008:administration & dosage; D002432:Cecum / Q000601:surgery; D005260:Female; D006801:Humans; D018836:Inflammation Mediators / Q000378:metabolism*; D064094:Interleukin-27 / Q000235:genetics / Q000378:metabolism*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008875:Middle Aged; D023421:Models, Animal; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D019138:Antibodies, Blocking; D018836:Inflammation Mediators; D064094:Interleukin-27