title: Effects ofL-arginine andN-nitro-L-arginine treatment on hemodynamics, DO2, VO 2, and extravascular lung water in a dog endotoxin shock model.
abstract: To verify the effect of nitric oxide pathway modification during sepsis, experiments were conducted in four groups of anesthetized dogs which received lipopolysaccharide (LPS) intravenously (group 1), 300 mg·kg(-1) ofL-arginine plus LPS (group 2), 20 mg·kg(-1) ofN-nitro-L-arginine plus LPS (L-NNA, group 3), and normal saline as the control group. Hemodynamic and oxygenation data as well as extravascular lung water (EVLW) were measured or calculated. The results showed thatL-arginine increases cardiac output index (CI) and decreased the peripheral vascular resistance index (PVRI) without a significant influence on oxygen extraction ratio (O2ER), oxygen delivery (DO2), or oxygen consumption (VO2). All of the untoward hemodynamic effects of LPS were exacerbated by the addition ofL-NNA. Therefore, as DO2 was significantlys decreased byL-NNA, and although O2ER was increased (insufficiently), VO2 was still decreased significantly. EVLW was markedly increased byL-NNA. These results support the hypothesis that inhibition of nitric oxide synthesis may exacerbate hemodynamic and oxygenation consequences in septic shock.
authors.affiliation: Department of Anesthesiology, First Hospital, Beijing Medical University, No. 8 Xishiku Street, 100034, Beijing, China.
authors.forename: X
authors.initials: X
authors.lastname: Wu
authors.forename: S
authors.initials: S
authors.lastname: Zhang
authors.forename: G
authors.initials: G
authors.lastname: Shan