title: The effect of acute exposure to hyperbaric oxygen on respiratory system mechanics in the rat.
abstract: OBJECTIVE
  This study was designed to investigate the possible effects of acute hyperbaric hyperoxia on respiratory mechanics of anaesthetised, positive-pressure ventilated rats.
  
  
  METHODS
  We measured respiratory mechanics by the end-inflation occlusion method in nine rats previously acutely exposed to hyperbaric hyperoxia in a standard fashion. The method allows the measurements of respiratory system elastance and of both the "ohmic" and of the viscoelastic components of airway resistance, which respectively depend on the newtonian pressure dissipation due to the ohmic airway resistance to air flow, and on the viscoelastic pressure dissipation caused by respiratory system tissues stress-relaxation. The activities of inducible and endothelial NO-synthase in the lung's tissues (iNOS and eNOS respectively) also were investigated. Data were compared with those obtained in control animals.
  
  
  RESULTS
  We found that the exposure to hyperbaric hyperoxia increased respiratory system elastance and both the "ohmic" and viscoelastic components of inspiratory resistances. These changes were accompanied by increased iNOS but not eNOS activities.
  
  
  CONCLUSIONS
  Hyperbaric hyperoxia was shown to acutely induce detrimental effects on respiratory mechanics. A possible causative role was suggested for increased nitrogen reactive species production because of increased iNOS activity.
authors.affiliation: Section Physiology, Department of Biomedical Sciences, University of Padova, Via Marzolo, 3, 35100, Padua, Italy, alessandro.rubini@unipd.it.
authors.forename: Alessandro
authors.initials: A
authors.lastname: Rubini
authors.forename: Andrea
authors.initials: A
authors.lastname: Porzionato
authors.forename: Susi
authors.initials: S
authors.lastname: Zara
authors.forename: Amelia
authors.initials: A
authors.lastname: Cataldi
authors.forename: Giacomo
authors.initials: G
authors.lastname: Garetto
authors.forename: Gerardo
authors.initials: G
authors.lastname: Bosco
mesh_terms: D000403:Airway Resistance / Q000502:physiology; D000818:Animals; D005260:Female; D006931:Hyperbaric Oxygenation; D018496:Hyperoxia / Q000503:physiopathology*; D008168:Lung / Q000378:metabolism / Q000503:physiopathology; D008297:Male; D023421:Models, Animal; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D052250:Nitric Oxide Synthase Type III / Q000378:metabolism; D051381:Rats; D011922:Rats, Mutant Strains; D015656:Respiratory Mechanics / Q000502:physiology*; D012137:Respiratory System / Q000378:metabolism / Q000503:physiopathology*
chemical_list: D052247:Nitric Oxide Synthase Type II; D052250:Nitric Oxide Synthase Type III