title: Tissue oxygenation during exercise measured with NIRS: reproducibility and influence of wavelengths.
abstract: Near-infrared spectroscopy (NIRS) is widely used for the measurement of skeletal muscle oxygenation during exercise as it reflects muscle metabolism, and most studies report a large variability between subjects. Here we assess the data quality of tissue oxygen saturation (SO2) and oxygenated (oxyHb) and deoxygenated (deoxyHb) haemoglobin concentrations recorded during an incremental cycling protocol in nine healthy volunteers. The protocol was repeated three times on the same day and a fourth session on a different day to estimate the reproducibility of the method with a broadband, spatially resolved spectroscopy (SRS) system. We found that the inter-subject variation in SO2 (standard deviation ≈ 6 %) was considerably larger than the reproducibility (≈ 1.5 %) both for the same-day and different-day tests. The reproducibility of changes in SO2 was better than 1 %.
authors.affiliation: RheinAhrCampus, University of Applied Sciences Koblenz, Remagen, Germany.
authors.forename: Erwin
authors.initials: E
authors.lastname: Gerz
authors.affiliation: RheinAhrCampus, University of Applied Sciences Koblenz, Remagen, Germany.
authors.forename: Dmitri
authors.initials: D
authors.lastname: Geraskin
authors.affiliation: Institute of Sports Medicine, Ruhr University Bochum, Bochum, Germany.|Institute of Cardiology and Sports Medicine, German Sport University, Cologne, Germany.
authors.forename: Julia
authors.initials: J
authors.lastname: Franke
authors.affiliation: Institute of Sports Medicine, Ruhr University Bochum, Bochum, Germany.|Institute of Cardiology and Sports Medicine, German Sport University, Cologne, Germany.
authors.forename: Petra
authors.initials: P
authors.lastname: Platen
authors.affiliation: RheinAhrCampus, University of Applied Sciences Koblenz, Remagen, Germany.
authors.forename: André
authors.initials: A
authors.lastname: Steimers
authors.affiliation: RheinAhrCampus, University of Applied Sciences Koblenz, Remagen, Germany. kohl-bareis@rheinahrcampus.de.
authors.forename: Matthias
authors.initials: M
authors.lastname: Kohl-Bareis
mesh_terms: D000328:Adult; D015444:Exercise / Q000502:physiology*; D005260:Female; D006801:Humans; D008297:Male; D018482:Muscle, Skeletal / Q000098:blood supply / Q000378:metabolism; D010100:Oxygen / Q000097:blood / Q000378:metabolism*; D010101:Oxygen Consumption / Q000502:physiology*; D015203:Reproducibility of Results; D019265:Spectroscopy, Near-Infrared / Q000379:methods
chemical_list: D010100:Oxygen