title: Co-evolution of HAD phosphatase and hotdog-fold thioesterase domain function in the menaquinone-pathway fusion proteins BF1314 and PG1653.
abstract: The function of a Bacteroidetes menaquinone biosynthetic pathway fusion protein comprised of an N-terminal haloacid dehalogenase (HAD) family domain and a C-terminal hotdog-fold family domain is described. Whereas the thioesterase domain efficiently catalyzes 1,4-dihydroxynapthoyl-CoA hydrolysis, an intermediate step in the menaquinone pathway, the HAD domain is devoid of catalytic activity. In some Bacteroidetes a homologous, catalytically active 1,4-dihydroxynapthoyl-CoA thioesterase replaces the fusion protein. Following the gene fusion event, sequence divergence resulted in a HAD domain that functions solely as the oligomerization domain of an otherwise inactive thioesterase domain.
authors.affiliation: Department of Chemistry & Chemical Biology, University of New Mexico, Albuquerque, NM 87131, USA.
authors.forename: Min
authors.initials: M
authors.lastname: Wang
authors.forename: Feng
authors.initials: F
authors.lastname: Song
authors.forename: Rui
authors.initials: R
authors.lastname: Wu
authors.forename: Karen N
authors.initials: KN
authors.lastname: Allen
authors.forename: Patrick S
authors.initials: PS
authors.lastname: Mariano
authors.forename: Debra
authors.initials: D
authors.lastname: Dunaway-Mariano
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics; D041963:Bacteroidetes / Q000201:enzymology*; D053898:Biosynthetic Pathways; D020134:Catalytic Domain; D019143:Evolution, Molecular; D006867:Hydrolases / Q000737:chemistry / Q000235:genetics; D007700:Kinetics; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010744:Phosphoric Monoester Hydrolases / Q000737:chemistry* / Q000235:genetics; D054730:Protein Interaction Domains and Motifs; D055503:Protein Multimerization; D017433:Protein Structure, Secondary; D011993:Recombinant Fusion Proteins / Q000737:chemistry* / Q000235:genetics; D013869:Thiolester Hydrolases / Q000737:chemistry* / Q000235:genetics; D024482:Vitamin K 2 / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D011993:Recombinant Fusion Proteins; D024482:Vitamin K 2; D006867:Hydrolases; D013869:Thiolester Hydrolases; D010744:Phosphoric Monoester Hydrolases; C034507:2-haloacid dehalogenase
keywords: 14-Dihroxynapthoyl-coenzyme A; 2-(N-morpholino)ethanesulfonate; Bacteroides fragilis; CoA; Enzyme evolution; Enzyme superfamily; HAD; HEPES; Haloacid dehalogenase; IPTG; MES; N-(2-hydroxyethyl)piperazine-N′-2-ethanesulfonate; Porphyromonas gingivalis; coenzyme A; haloacid dehalogenase; isopropyl-1-thio-β-d-galactopyranoside