title: Detection of vanC1 gene transcription in vancomycin-susceptible Enterococcus faecalis.
abstract: Here we report the presence and expression levels of the vanC1 and vanC(2/3) genes in vancomycin-susceptible strains of Enterococcus faecalis. The vanC1 and vanC(2/3) genes were located in the plasmid DNA and on the chromosome, respectively. Specific mRNA of the vanC1 gene was detected in one of these strains. Additionally, analysis of the vanC gene sequences showed that these genes are related to the vanC genes of Enterococcus gallinarum and Enterococcus casseliflavus. The presence of vanC genes is useful for the identification of E. gallinarum and E. casseliflavus. Moreover, this is the first report of vanC mRNA in E. faecalis.
authors.affiliation: Programa de Pós-Graduação em Microbiologia Agrícola e do Ambiente, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brasil. tianedemoura@gmail.com
authors.forename: Tiane Martin de
authors.initials: TM
authors.lastname: Moura
authors.forename: Ana Paula Vaz
authors.initials: AP
authors.lastname: Cassenego
authors.forename: Fabrício Souza
authors.initials: FS
authors.lastname: Campos
authors.forename: Andrea Machado Leal
authors.initials: AM
authors.lastname: Ribeiro
authors.forename: Ana Cláudia
authors.initials: AC
authors.lastname: Franco
authors.forename: Pedro Alves
authors.initials: PA
authors.lastname: d'Azevedo
authors.forename: Jeverson
authors.initials: J
authors.lastname: Frazzon
authors.forename: Ana Paula Guedes
authors.initials: AP
authors.lastname: Frazzon
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000235:genetics*; D002645:Chickens; D002988:Cloaca / Q000382:microbiology; D004269:DNA, Bacterial / Q000032:analysis; D052978:Disk Diffusion Antimicrobial Tests; D013293:Enterococcus faecalis / Q000187:drug effects* / Q000235:genetics* / Q000302:isolation & purification; D005798:Genes, Bacterial / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D014640:Vancomycin / Q000494:pharmacology*; D020713:Vancomycin Resistance / Q000235:genetics*
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D004269:DNA, Bacterial; C079914:VanC protein, Bacteria; D014640:Vancomycin