title: Statistics of time-dependent rupture of single ds-DNA.
abstract: Double-stranded (ds-) DNA molecules were stretched and ruptured on molecularly modified graphite surfaces with a scanning force microscope (SFM) exerting a force parallel to the surface. The stretching force was either large enough to break the molecule immediately or compensated by the elastic restoring force of the DNA backbone, which stabilized the molecular length. However, the size-stabilized molecules broke gradually from longer molecules to shorter ones with time. The breakage of different lengths of stabilized molecules was recorded in order to study time-dependent mechanical properties of the molecules under constant forces. From these data, a relatively high rate constant, k0 = (2.2 ± 0.1) × 10(-7) s(-1), was calculated. Moreover, we found a nonlinear stress-strain dependence of DNA on the surface which we attributed to DNA conformational transition. Assuming that the structural transition on the surface is similar to that in solution we estimated the forces needed to stretch the molecules and thereby verify the estimation of the activation energy barrier.
authors.affiliation: Department of Physics, Humboldt-Universität zu Berlin, Newtonstr. 15, D-12489 Berlin, Germany.
authors.forename: Hua
authors.initials: H
authors.lastname: Liang
authors.forename: Nikolai
authors.initials: N
authors.lastname: Severin
authors.forename: Simon
authors.initials: S
authors.lastname: Fugmann
authors.forename: Igor M
authors.initials: IM
authors.lastname: Sokolov
authors.forename: Jürgen P
authors.initials: JP
authors.lastname: Rabe
mesh_terms: D004247:DNA / Q000737:chemistry*; D043243:DNA Sequence, Unstable; D008855:Microscopy, Electron, Scanning; D008968:Molecular Conformation; D013223:Statistics as Topic; D013997:Time Factors
chemical_list: D004247:DNA