title: Determination of chidamide in rat plasma by LC-MS and its application to pharmacokinetics study.
abstract: A sensitive and selective liquid chromatography mass spectrometry method for determination of chidamide in rat plasma was developed. After addition of linezolid as internal standard, protein precipitation by acetonitrile-methanol (9:1, v/v) was used as sample preparation. Chromatographic separation was achieved on a Zorbax SB-C18 (2.1 × 150 mm, 5 µm) column with acetonitrile-0.1% formic acid as mobile phase with gradient elution. An electrospray ionization source was applied and operated in positive ion mode; selective ion monitoring mode was used for quantification using target fragment ions m/z 391.5 for chidamide and m/z 338.5 for the IS. Calibration plots were linear over the range of 10-2000 ng/mL for chidamide in rat plasma. The lower limit of quantification for chidamide was 10 ng/mL. The mean recovery of chidamide in plasma was in the range of 86.6-92.1%. The coefficients of variation of intra-day and inter-day precision were both <12%. This method is simple and sensitive and was applied successfully in a pharmacokinetic study of chidamide to rats.
authors.affiliation: Analytical and Testing Center of Wenzhou Medical University, Wenzhou, 325035, China.
authors.forename: Xianqin
authors.initials: X
authors.lastname: Wang
authors.forename: Mengchun
authors.initials: M
authors.lastname: Chen
authors.forename: Congcong
authors.initials: C
authors.lastname: Wen
authors.forename: Qingwei
authors.initials: Q
authors.lastname: Zhang
authors.forename: Jianshe
authors.initials: J
authors.lastname: Ma
mesh_terms: D000631:Aminopyridines / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D000818:Animals; D001549:Benzamides / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D002853:Chromatography, Liquid / Q000379:methods*; D004355:Drug Stability; D016014:Linear Models; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
chemical_list: D000631:Aminopyridines; D001549:Benzamides; C547816:N-(2-amino-5-fluorobenzyl)-4-(N-(pyridine-3-acrylyl)aminomethyl)benzamide
keywords: LC-MS; chidamide; pharmacokinetics; rat plasma