title: Crystallization and preliminary X-ray diffraction analysis of a thermostable GDSL-family esterase, EstL5, from Geobacillus thermodenitrificans T2.
abstract: The novel thermostable esterase EstL5 belonging to the GDSL family exhibits a unique cold-adaptation feature at low temperatures. To better understand its biochemical and enzymatic properties, recombinant EstL5 protein was purified and crystallized using the vapour-diffusion method. The EstL5 crystals diffracted X-rays to 2.79 Å resolution using a synchrotron-radiation source, belonged to the tetragonal space group P41212 or P43212, with unit-cell parameters a = b = 101.51, c = 124.22 Å, and are expected to contain two molecules in each asymmetric unit. To obtain initial phases, selenomethionyl-substituted protein was overproduced. Purified SeMet-labelled EstL5 protein was crystallized and formed crystals that diffracted to a resolution of 3.0 Å.
authors.affiliation: State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, 220 Handan Road, Shanghai 200433, People's Republic of China.
authors.forename: Zhenxing
authors.initials: Z
authors.lastname: Yang
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhang
authors.forename: Rui
authors.initials: R
authors.lastname: Qiu
authors.forename: Jing
authors.initials: J
authors.lastname: Huang
authors.forename: Chaoneng
authors.initials: C
authors.lastname: Ji
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D002087:Butyrates / Q000378:metabolism; D003460:Crystallization; D018360:Crystallography, X-Ray; D004950:Esterases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D004952:Esters / Q000378:metabolism; D056506:Geobacillus / Q000201:enzymology*; D008049:Lipase / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D008958:Models, Molecular; D009596:Nitrophenols / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D013329:Structure-Activity Relationship
chemical_list: D001426:Bacterial Proteins; D002087:Butyrates; D004952:Esters; D009596:Nitrophenols; D011994:Recombinant Proteins; D004950:Esterases; D008049:Lipase; C428386:thermostable lipase
keywords: EstL5; GDSL esterases; Geobacillus thermodenitrificans; thermostability