title: HMDO-promoted peptide and protein synthesis in ionic liquids.
abstract: Hexamethyldisiloxane (HMDO) has been developed to efficiently promote the metal-free direct coupling of an amino function of one cysteine-free peptide or protein and a C-terminal thioester of the second peptide in ionic liquids. The amide-coupling reaction proceeds smoothly under mild conditions to afford the corresponding products in good to excellent yields (63-94%). Peptide couplings were also achieved using in-situ-generated thioesters by the thioesterification of oxo esters.
authors.affiliation: Key Laboratory of Combinatorial Biosynthesis and Drug Discovery (Wuhan University), Ministry of Education, and Wuhan University School of Pharmaceutical Sciences, Wuhan, 430071, PR China.
authors.forename: Jianli
authors.initials: J
authors.lastname: Duan
authors.forename: Yao
authors.initials: Y
authors.lastname: Sun
authors.forename: Hao
authors.initials: H
authors.lastname: Chen
authors.forename: Guofu
authors.initials: G
authors.lastname: Qiu
authors.forename: Haibing
authors.initials: H
authors.lastname: Zhou
authors.forename: Ting
authors.initials: T
authors.lastname: Tang
authors.forename: Zixin
authors.initials: Z
authors.lastname: Deng
authors.forename: Xuechuan
authors.initials: X
authors.lastname: Hong
mesh_terms: D052578:Ionic Liquids / Q000737:chemistry*; D008958:Models, Molecular; D015394:Molecular Structure; D010455:Peptides / Q000138:chemical synthesis* / Q000737:chemistry; D011506:Proteins / Q000138:chemical synthesis* / Q000737:chemistry; D012833:Siloxanes / Q000737:chemistry*
chemical_list: D052578:Ionic Liquids; D010455:Peptides; D011506:Proteins; D012833:Siloxanes; C015917:hexamethyldisiloxane