title: Bacterial communities predominant in the degradation of 13C(4)-4,5,9,10-pyrene during composting.
abstract: An in-vessel composting bioremediation of (13)C4-4,5,9,10-pyrene and unlabeled pyrene spiked soil amended with fresh wastes was investigated by DNA-based stable isotope probing (SIP) of active bacteria involved. Highest dissipation of (13)C4-pyrene was detected at 55 °C after 42 days composting. The active bacterial communities in the composting changed over time, showing a distinct difference among different stages. α-, β-, γ-Proteobacteria, and Actinobacteria were detected mainly involving in pyrene degradation at 38 °C over 14 days composting. Streptomyces appeared to dominate the pyrene degradation at 55 °C. β- and γ-Proteobacteria and Actinobacteria were the dominant pyrene degraders at 70 °C after 42 days composting and at 38 °C after 60 days composting. The results of this study suggest the pyrene degradation was performed by phylogenetically distinct bacterial guilds from the phyla Actinobacteria and Proteobacteria during in-vessel composition processes.
authors.affiliation: Key Laboratory of Urban Environment and Health Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, China.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Peng
authors.forename: Yuan
authors.initials: Y
authors.lastname: Zhang
authors.forename: Jianqiang
authors.initials: J
authors.lastname: Su
authors.forename: Qiongfen
authors.initials: Q
authors.lastname: Qiu
authors.forename: Zhongjun
authors.initials: Z
authors.lastname: Jia
authors.forename: Yong-Guan
authors.initials: YG
authors.lastname: Zhu
mesh_terms: D001419:Bacteria / Q000145:classification / Q000378:metabolism*; D002247:Carbon Isotopes / Q000378:metabolism*; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D012150:Polymorphism, Restriction Fragment Length; D011721:Pyrenes / Q000378:metabolism*; D012987:Soil
chemical_list: D002247:Carbon Isotopes; D011721:Pyrenes; D012987:Soil; C030984:pyrene
keywords: Community structure; Composting; Pyrene degradation; Stable isotope probing (SIP); Temperature