title: Enhanced 1,2-dichloroethane degradation in heavy metal co-contaminated wastewater undergoing biostimulation and bioaugmentation.
abstract: Biostimulation, bioaugmentation and dual-bioaugmentation strategies were investigated in this study for efficient bioremediation of water co-contaminated with 1,2-dichloroethane (1,2-DCA) and heavy metals, in a microcosm set-up. 1,2-DCA concentration was periodically measured in the microcosms by gas chromatographic analysis of the headspace samples, while bacterial population and diversity were determined by standard plate count technique and Polymerase chain reaction and denaturing gradient gel electrophoresis (PCR-DGGE) analysis, respectively. Dual-bioaugmentation, proved to be most effective exhibiting 22.43%, 26.54%, 19.58% and 30.49% increase in 1,2-DCA degradation in microcosms co-contaminated with As(3+), Cd(2+), Hg(2+) and Pb(2+), respectively, followed by bioaugmentation and biostimulation. Dual-bioaugmented microcosms also exhibited the highest increase in the biodegradation rate constant (k1) resulting in 1.76-, 2-, 1.7- and 2.1-fold increase in As(3+), Cd(2+), Hg(2+) and Pb(2+) co-contaminated microcosms respectively, compared to the untreated microcosms. Dominant bacterial strains obtained from the co-contaminated microcosms were found to belong to the genera Burkholderia, Pseudomonas, Bacillus, Enterobacter and Bradyrhizobium, previously reported for 1,2-DCA and other chlorinated compounds degradation. PCR-DGGE analysis revealed variation in microbial diversity over time in the different co-contaminated microcosms. Results obtained in this study have significant implications for developing innovative bioremediation strategies for treating water co-contaminated with chlorinated organics and heavy metals.
authors.affiliation: Discipline of Microbiology, School of Life Sciences, University of KwaZulu-Natal (Westville Campus), Private Bag X54001, Durban 4000, South Africa.
authors.forename: Ashmita
authors.initials: A
authors.lastname: Arjoon
authors.forename: Ademola O
authors.initials: AO
authors.lastname: Olaniran
authors.forename: Balakrishna
authors.initials: B
authors.lastname: Pillay
mesh_terms: D001419:Bacteria / Q000378:metabolism; D001673:Biodegradation, Environmental; D005025:Ethylene Dichlorides / Q000032:analysis* / Q000378:metabolism; D019216:Metals, Heavy / Q000032:analysis* / Q000378:metabolism; D014865:Waste Disposal, Fluid / Q000379:methods*; D062065:Wastewater / Q000737:chemistry*; D014871:Water Microbiology; D014874:Water Pollutants, Chemical / Q000032:analysis* / Q000378:metabolism
chemical_list: D005025:Ethylene Dichlorides; D019216:Metals, Heavy; D062065:Wastewater; D014874:Water Pollutants, Chemical; C024565:ethylene dichloride
keywords: 1,2-Dichloroethane; Biostimulation; Co-contamination; Dual-bioaugmentation; Heavy metals; PCR–DGGE