title: Suppression of Arabidopsis RING E3 ubiquitin ligase AtATL78 increases tolerance to cold stress and decreases tolerance to drought stress.
abstract: AtATL78 is an Arabidopsis RING E3 ubiquitin ligase. RT-PCR and promoter-GUS assays revealed that AtATL78 was up-regulated by cold stress and down-regulated by drought. AtATL78 was localized at the plasma-membrane. Suppression of AtATL78 increased tolerance to cold stress but decreased tolerance to drought. Our data suggests that AtATL78 is a negative regulator of cold stress response and a positive regulator of drought stress response in Arabidopsis. These results further suggest that AtATL78 plays opposing roles in cold and drought stress responses.
authors.affiliation: Department of Systems Biology, College of Life Science and Biotechnology, Yonsei University, Seoul 120-749, Republic of Korea.
authors.forename: Soo Jin
authors.initials: SJ
authors.lastname: Kim
authors.forename: Woo Taek
authors.initials: WT
authors.lastname: Kim
mesh_terms: D017360:Arabidopsis / Q000201:enzymology*; D029681:Arabidopsis Proteins / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D003080:Cold Temperature; D055864:Droughts; D018506:Gene Expression Regulation, Plant; D005966:Glucuronidase / Q000378:metabolism; D010641:Phenotype; D018515:Plant Leaves / Q000378:metabolism; D030821:Plants, Genetically Modified; D034622:RNA Interference; D017382:Reactive Oxygen Species / Q000378:metabolism; D013312:Stress, Physiological; D025801:Ubiquitin / Q000378:metabolism; D044767:Ubiquitin-Protein Ligases / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; D017382:Reactive Oxygen Species; D025801:Ubiquitin; C583897:ATL78 protein, Arabidopsis; D044767:Ubiquitin-Protein Ligases; D005966:Glucuronidase
keywords: 3,3′-diaminobenzidine; ABA; ATL; ATL RING E3 Ub ligase; Arabidopsis; Arabidopsis Tóxicos en Levadura; Arabidopsis thaliana ABA-insensitive RING protein; Arabidopsis thaliana RING domain-of-unknown-function; AtAIRP; AtRDUF; C-repeat (CRT)-binding factor; CBF; CRWL; Cold response; DAB; Drought response; HECT; HOS1; ICE1; Loss-of-function mutant; RD29; RING; RNAi knock-down line; ROS; Ub; abscisic acid; cut rosette water loss; high expression of osmotically responsive gene 1; inducer of CBF expression 1; reactive oxygen species; really interesting new gene; responsive to dehydration 29A; sGFP; soluble green fluorescent protein; the homologous to E6-AP carboxyl terminus; ubiquitin