title: Mining of a streptothricin gene cluster from Streptomyces sp. TP-A0356 genome via heterologous expression.
abstract: Streptothricins (STs) are used commercially to treat bacterial and fungal diseases in agriculture. Mining of the sequenced microbial genomes uncovered two cryptic ST clusters from Streptomyces sp. C and Streptomyces sp. TP-A0356. The ST cluster from S. sp. TP-A0356 was verified by successful heterologous expression in Streptomyces coelicolor M145. Two new ST analogs were produced together with streptothricin F and streptothricin D in the heterologous host. The ST cluster was further confirmed by inactivation of gene stnO, which was proposed encoding an aminomutase supplying β-lysines for the poly-β-Lys chain formation. A putative biosynthetic pathway for STs is proposed based on bioinformatics analyses of the ST genes and experimental evidence.
authors.affiliation: State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Jine
authors.initials: J
authors.lastname: Li
authors.forename: Zhengyan
authors.initials: Z
authors.lastname: Guo
authors.forename: Wei
authors.initials: W
authors.lastname: Huang
authors.forename: Xiangxi
authors.initials: X
authors.lastname: Meng
authors.forename: Guomin
authors.initials: G
authors.lastname: Ai
authors.forename: Gongli
authors.initials: G
authors.lastname: Tang
authors.forename: Yihua
authors.initials: Y
authors.lastname: Chen
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry; D001412:Bacillus subtilis / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D019295:Computational Biology; D005285:Fermentation; D015964:Gene Expression Regulation, Bacterial; D005816:Genetic Complementation Test; D016680:Genome, Bacterial; D005810:Multigene Family; D016296:Mutagenesis; D009154:Mutation; D017422:Sequence Analysis, DNA; D013210:Staphylococcus / Q000235:genetics* / Q000378:metabolism; D013309:Streptothricins / Q000737:chemistry / Q000378:metabolism*; D013997:Time Factors
chemical_list: D000900:Anti-Bacterial Agents; C009156:streptothricin F; D013309:Streptothricins