title: [Effects of human umbilical cord mesenchymal stem cells on the proliferation of hematopoietic malignancies].
abstract: OBJECTIVE
  To study the effects of human umbilical cord mesenchymal stem cells (UC-MSCs) on proliferation of eight tumor cell lines from leukemia, lymphoma and multiple myeloma in vitro.
  
  
  METHODS
  Tumor cells were labeled with carboxyfluorescein diacetate succinimidyl ester (CFDA-SE). UC-MSCs were co-cultured with tumor cells at different ratios as experimental groups, meanwhile, tumor cells were cultured without UC-MSCs as control groups. After three days, mean fluorescence intensity and cell division generations of the tumor cells were measured by flow cytometry.
  
  
  RESULTS
  UC-MSCs inhibited the proliferation of HL60, THP1, K562 and RPMI8226 cell lines, but promoted the proliferation of Raji and NCIH929 cell lines. UC-MSCs promoted the proliferation of Jurkat cells only at 1:1 ratio; as for U937 cells, UC-MSCs inhibited the proliferation at 2:1 ratio (UC-MSCs: U937), promoted proliferation at 1:4 and 1:16, whereas had no obvious effect at 1:1.
  
  
  CONCLUSIONS
  UC-MSCs have different effects on the proliferation of different hematopoietic tumor cell lines. They have no promoting effects on four leukemic cell lines, but have bidirectional (promotion/inhibition) effects on lymphoma and multiple myeloma cell lines. The U937 cell line may serve as a good model for the mechanism study of this contradictory phenomenon.
authors.affiliation: General Hospital of PLA, Beijing 100853, China.
authors.forename: Yilin
authors.initials: Y
authors.lastname: Pang
authors.forename: Yongyong
authors.initials: Y
authors.lastname: Tang
authors.forename: Qian
authors.initials: Q
authors.lastname: Li
authors.forename: Jing
authors.initials: J
authors.lastname: Xie
authors.forename: Xuefeng
authors.initials: X
authors.lastname: Zang
authors.forename: Tingting
authors.initials: T
authors.lastname: Liu
authors.forename: Bin
authors.initials: B
authors.lastname: Zhang
authors.forename: Hu
authors.initials: H
authors.lastname: Chen
mesh_terms: D002455:Cell Division; D049109:Cell Proliferation; D018920:Coculture Techniques; D005312:Fetal Blood / Q000166:cytology*; D005434:Flow Cytometry; D018922:HL-60 Cells; D019337:Hematologic Neoplasms / Q000378:metabolism*; D006801:Humans; D019169:Jurkat Cells; D020014:K562 Cells; D059630:Mesenchymal Stem Cells / Q000378:metabolism*; D013997:Time Factors; D020298:U937 Cells