title: Identification of three novel human leukocyte antigen alleles, HLA-B*58:43, HLA-C*03:190, and HLA-DPA1*01:12, in an East African cohort.
abstract: Three novel human leukocyte antigen (HLA) alleles were identified using a sequence-based typing of HLA class I and class II alleles of 1867 participants from a male circumcision cohort in Kenya. The new alleles were first identified by sequencing and then confirmed by cloning the polymerase chain reaction (PCR) products and sequencing multiple clones. HLA-B*58:43 was identical to HLA-B*58:02 with the exception of a nucleotide change at codon 125 in exon 3 (GCC→ACC), and resulted in the amino acid change from Alanine to Threonine. HLA-C*03:190 was identical to HLA-C*03:02:01 with the exception of a nucleotide change at codon 131 in exon 3 (CGC→TGC), and resulted in the amino acid change from Arginine to Cysteine. HLA-DPA1*01:12 was identical to HLA-DPA1*01:03:01:01 with the exception of a nucleotide change at codon 66 in exon 2 (TTG→TCG), and resulted in the amino acid change from Leucine to Serine.
authors.affiliation: HIV and Human Genetics, National Microbiology Laboratory, Winnipeg, Canada.
authors.forename: M
authors.initials: M
authors.lastname: Nykoluk
authors.forename: R C
authors.initials: RC
authors.lastname: Bailey
authors.forename: S
authors.initials: S
authors.lastname: Moses
authors.forename: F A
authors.initials: FA
authors.lastname: Plummer
authors.forename: M
authors.initials: M
authors.lastname: Luo
mesh_terms: D000351:Africa, Eastern; D000483:Alleles; D019943:Amino Acid Substitution; D001483:Base Sequence; D002944:Circumcision, Male; D003062:Codon; D005091:Exons; D015235:HLA-B Antigens / Q000235:genetics* / Q000276:immunology; D015236:HLA-C Antigens / Q000235:genetics* / Q000276:immunology; D059867:HLA-DP alpha-Chains / Q000235:genetics* / Q000276:immunology; D006650:Histocompatibility Testing; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
chemical_list: D003062:Codon; D015235:HLA-B Antigens; D015236:HLA-C Antigens; D059867:HLA-DP alpha-Chains; C071172:HLA-DPA1 antigen
keywords: B*58:43; C*03:190; DPA1*01:12; HLA-DPA1; Kenyan; human leukocyte antigen-B; human leukocyte antigen-C; taxonomy-based sequence analysis