title: Immunocompatibility evaluation of hydrogel-coated polyimide implants for applications in regenerative medicine.
abstract: Immunocompatibility of gelatin-based hydrogels to be applied as implant coatings for local regenerative treatment has been studied. First, the bio- and immuno-acceptability of the methacrylamide-modified gelatin hydrogels per se was screened. The results indicated that the hydrogels support cell growth. Metabolic activity of normal cells and permanent cell lines representing various cell types (endothelial, epithelial, fibroblast, and monocyte/macrophage) cultivated on the gelatin hydrogels was moderately lower compared to cells cultivated on tissue culture plastic. The cells cultivated on the hydrogels produced identical cytokines as the control cells although at lower levels. Importantly, no inflammatory activity, measured by nitric oxide and pro-inflammatory cytokine (IL-1α, IL-6, and TNFα) production, was observed in peritoneal cells and monocyte/macrophage RAW 264.7 cell line cultivated on the hydrogels. Finally, polyimide (PI) implantable membranes were surface-modified with gelatin hydrogels and screened for their in vivo immunocompatibility. Their histological examination performed after subcutaneous implantation in mice produced a sound proof of immunoacceptability. Normal tissue repair, mild cellular infiltration and edema mainly induced by the surgery were observed after 2 and 6 days. No adverse tissue responses were induced by the implants. Analysis performed after 4 and 9 weeks indicated areas of foreign body granuloma without formation of a fibrous capsule.
authors.affiliation: Division of Immunology and Gnotobiology, Institute of Microbiology, Academy of Sciences of the Czech Republic, v.v.i., Videnska 1083, 142 20, Prague, 4, Czech Republic.
authors.forename: Milada
authors.initials: M
authors.lastname: Sirova
authors.forename: Sandra
authors.initials: S
authors.lastname: Van Vlierberghe
authors.forename: Veronika
authors.initials: V
authors.lastname: Matyasova
authors.forename: Pavel
authors.initials: P
authors.lastname: Rossmann
authors.forename: Etienne
authors.initials: E
authors.lastname: Schacht
authors.forename: Peter
authors.initials: P
authors.lastname: Dubruel
authors.forename: Blanka
authors.initials: B
authors.lastname: Rihova
mesh_terms: D000178:Acrylamides / Q000737:chemistry* / Q000276:immunology; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry* / Q000378:metabolism; D002460:Cell Line; D049109:Cell Proliferation; D016207:Cytokines / Q000276:immunology; D005347:Fibroblasts / Q000166:cytology / Q000276:immunology; D005780:Gelatin / Q000737:chemistry* / Q000276:immunology; D006801:Humans; D020100:Hydrogels / Q000737:chemistry* / Q000378:metabolism; D008264:Macrophages / Q000166:cytology / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D019736:Prostheses and Implants; D044968:Regenerative Medicine
chemical_list: D000178:Acrylamides; D001672:Biocompatible Materials; D016207:Cytokines; D020100:Hydrogels; D005780:Gelatin; C045985:methacrylamide
keywords: hydrogel; immunocompatibility; methacrylamide-modified gelatin; polyimide implant; subcutaneous implantation