title: Abnormal expression of miR-301a in gastric cancer associated with progression and poor prognosis.
abstract: OBJECTIVE
  miR-301a is significantly overexpressed in many cancers. However, its expression and biological role in gastric cancer remain poorly understood. We investigated microRNA-301a (miR-301a) expression in gastric cancer and determined its effects on cancer cell behavior and its clinical significance in the development and progression of gastric cancer.
  
  
  METHODS
  We determined miR-301a expression in gastric tumors and gastric cancer cell lines by reverse transcription-polymerase chain reaction. The effects of miR-301a on cell clone formation, migration, and invasion of HGC-27 and SGC-7901 cells were detected following transfection of an miR-301a inhibitor. miR-301a expression in a 304-tissue gastric cancer microarray was determined by in situ hybridization and its role in progression and prognosis was analyzed.
  
  
  RESULTS
  miR-301a was upregulated in gastric tumor tissues and cell lines. Down-regulation of miR-301a significantly inhibited cell clone formation, migration, and invasion of HGC-27and SGC-7901 cells. Overexpression of miR-301a in primary gastric cancer tissues was associated with tumor size, invasion depth, lymph node metastasis, and TNM stage.
  
  
  CONCLUSIONS
  miR-301a overexpression correlated with TNM stage and prognosis, suggesting that miR-301a is involved in cellular clone formation, migration, and invasion in vitro and may play an important role in the clinical progression and prognosis of gastric cancer.
authors.affiliation: Wenzhou Medical College, Wenzhou, PR China.
authors.forename: Xiao-Dong
authors.initials: XD
authors.lastname: Xu
authors.forename: Xu-Jun
authors.initials: XJ
authors.lastname: He
authors.forename: Hou-Quan
authors.initials: HQ
authors.lastname: Tao
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: Yuan-Yu
authors.initials: YY
authors.lastname: Wang
authors.forename: Zai-Yuan
authors.initials: ZY
authors.lastname: Ye
authors.forename: Zhong-Sheng
authors.initials: ZS
authors.lastname: Zhao
mesh_terms: D000328:Adult; D000368:Aged; D045744:Cell Line, Tumor; D018450:Disease Progression; D005260:Female; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000502:physiology*; D008875:Middle Aged; D011379:Prognosis; D013274:Stomach Neoplasms / Q000235:genetics / Q000473:pathology*; D046888:Tissue Array Analysis; D015854:Up-Regulation
chemical_list: C550735:MIRN301A microRNA, human; D035683:MicroRNAs
keywords: gastric cancer; miR-301a; prognosis; progression