title: Integrated fluorescence correlation spectroscopy device for point-of-care clinical applications.
abstract: We describe an optical system which reduces the cost and complexity of fluorescence correlation spectroscopy (FCS), intended to increase the suitability of the technique for clinical use. Integration of the focusing optics and sample chamber into a plastic component produces a design which is simple to align and operate. We validate the system by measurements on fluorescent dye, and compare the results to a commercial instrument. In addition, we demonstrate its application to measurements of concentration and multimerization of the clinically relevant protein von Willebrand factor (vWF) in human plasma.
authors.affiliation: Department of Biomedical Engineering, Yale School of Engineering and Applied Science, New Haven, CT 06510, USA.
authors.forename: Eben
authors.initials: E
authors.lastname: Olson
authors.forename: Richard
authors.initials: R
authors.lastname: Torres
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Levene
keywords: (170.1610) Clinical applications; (170.4580) Optical diagnostics for medicine; (170.6280) Spectroscopy, fluorescence and luminescence; (220.0220) Optical design and fabrication