title: Development- and activity-dependent expression of clusterin in the mouse olfactory bulb.
abstract: Clusterin, a protein involved in many biological processes, is expressed broadly in the central nervous system, but its functions remain largely unknown. As preparations for elucidating some possible functions, we examined the spatiotemporal expression patterns of clusterin in the mouse olfactory bulb at different developmental stages and under different neuronal activity levels. Our results revealed a dynamic expression of the protein during development. Clusterin signal was seemingly diffuse during the early stages of development, shifted to the cell somas later and then predominantly to the axons of projection neurons in the adult stage, with a transition point at approximately postnatal day 18. The effects of olfactory deficits on the clusterin expression level in an anosmic mouse model were neuron-specific: the signals increased remarkably from faint to strong in olfactory sensory neurons, reduced considerably from moderate/strong to faint in the centrifugal projection neurons, decreased moderately from moderate to faint in the local bulbar projection neurons, and remained intense in long-distance bulbar projection neurons. These results showed that clusterin expression is modulated dynamically during development and by sensory activity. These findings deepen our understanding of this broadly expressed protein.
authors.affiliation: The State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, Wuhan Institute of Physics and Mathematics, Chinese Academy of Sciences, Wuhan 430071, China; University of Chinese Academy of Sciences, Beijing 100049, China.
authors.forename: Rui-Qi
authors.initials: RQ
authors.lastname: Wu
authors.forename: Zhi-Xiang
authors.initials: ZX
authors.lastname: Xu
authors.forename: Xiao-Ping
authors.initials: XP
authors.lastname: Rao
authors.forename: Fu-Qiang
authors.initials: FQ
authors.lastname: Xu
mesh_terms: D000367:Age Factors; D000818:Animals; D000831:Animals, Newborn; D051152:Clusterin / Q000378:metabolism*; D054815:Cyclic Nucleotide-Gated Cation Channels / Q000172:deficiency / Q000235:genetics; D004622:Embryo, Mammalian; D005260:Female; D007839:Functional Laterality / Q000235:genetics; D018507:Gene Expression Regulation, Developmental / Q000235:genetics / Q000502:physiology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008970:Molecular Weight; D009830:Olfactory Bulb / Q000196:embryology / Q000254:growth & development / Q000378:metabolism; D011247:Pregnancy; D015398:Signal Transduction / Q000235:genetics / Q000502:physiology; D020825:T-Box Domain Proteins / Q000378:metabolism; D000098815:T-bet Transcription Factor
chemical_list: C495243:Clu protein, mouse; D051152:Clusterin; C518240:Cnga2 protein, mouse; D054815:Cyclic Nucleotide-Gated Cation Channels; D020825:T-Box Domain Proteins; D000098815:T-bet Transcription Factor
keywords: Anosmic; Apolipoprotein; Immunohistochemistry; Olfactory system; Spatiotemporal distribution