title: Interaction of CdSe/ZnS quantum dots with the marine diatom Phaeodactylum tricornutum and the green alga Dunaliella tertiolecta: a biophysical approach.
abstract: In this study, we investigated the interaction of nanoparticles, such as CdSe/ZnS quantum dots (QDs), with the marine diatom Phaeodactylum tricornutum and the green alga Dunaliella tertiolecta, as biological models in the marine environment. Fluorescence kinetics measurements indicated that 30min after dispersion in seawater QDs lost the 60% of the initial emission intensity, possibly due to the occurrence of aggregation processes. However, the presence of algae seemed to mitigate this effect. By using confocal microscopy, we highlighted the presence of QDs adsorbed on the surface of both algae, but not inside the cells. The toxicity of QDs was evaluated in terms of inhibition of growth rate, oxidative stress, and lipid peroxidation. QDs in the range of 1-2.5nM gradually inhibited the growth rate of P. tricornutum and increased the oxidative stress, as evinced by the increase in lipid peroxidation, reactive oxygen species (ROS) production and activity of two main antioxidant enzymes (superoxide dismutase and catalase). On the contrary, QDs did not inhibit the growth rate of D. tertiolecta, at most a modest stimulation was observed in the range of 0.5-2nM, suggesting a hormetic response. No effect in the parameters indicating oxidative stress was observed in the green alga. In conclusion our results showed that the biological effects were species-specific.
authors.affiliation: Istituto di Biofisica, CNR, via G. Moruzzi 1, 56124 Pisa, Italy. Electronic address: elisabetta.morelli@pi.ibf.cnr.it.
authors.forename: Elisabetta
authors.initials: E
authors.lastname: Morelli
authors.forename: Elisa
authors.initials: E
authors.lastname: Salvadori
authors.forename: Ranieri
authors.initials: R
authors.lastname: Bizzarri
authors.forename: Patrizia
authors.initials: P
authors.lastname: Cioni
authors.forename: Edi
authors.initials: E
authors.lastname: Gabellieri
mesh_terms: D019187:Cadmium Compounds / Q000737:chemistry; D002374:Catalase / Q000378:metabolism; D000460:Chlorophyta / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D017377:Diatoms / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D007700:Kinetics; D018613:Microscopy, Confocal; D018384:Oxidative Stress / Q000187:drug effects; D045663:Quantum Dots / Q000737:chemistry / Q000633:toxicity*; D017382:Reactive Oxygen Species / Q000378:metabolism; D012623:Seawater / Q000737:chemistry; D018036:Selenium Compounds / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D013440:Sulfides / Q000737:chemistry; D013482:Superoxide Dismutase / Q000378:metabolism; D017967:Zinc Compounds / Q000737:chemistry
chemical_list: D019187:Cadmium Compounds; D017382:Reactive Oxygen Species; D018036:Selenium Compounds; D013440:Sulfides; D017967:Zinc Compounds; C058667:cadmium selenide; D002374:Catalase; D013482:Superoxide Dismutase; C031238:zinc sulfide
keywords: Confocal microscopy; Ecotoxicity; Fluorescence of nanoparticle; Marine microalgae; Quantum dot