title: Application of an efficient gene targeting system linking secondary metabolites to their biosynthetic genes in Aspergillus terreus.
abstract: Nonribosomal peptides (NRPs) are natural products biosynthesized by NRP synthetases. A kusA-, pyrG- mutant strain of Aspergillus terreus NIH 2624 was developed that greatly facilitated the gene targeting efficiency in this organism. Application of this tool allowed us to link four major types of NRP-related secondary metabolites to their responsible genes in A. terreus. In addition, an NRP affecting melanin synthesis was also identified in this species.
authors.affiliation: Department of Pharmacology and Pharmaceutical Sciences, School of Pharmacy, University of Southern California, Los Angeles, California 90089, USA.
authors.forename: Chun-Jun
authors.initials: CJ
authors.lastname: Guo
authors.forename: Benjamin P
authors.initials: BP
authors.lastname: Knox
authors.forename: James F
authors.initials: JF
authors.lastname: Sanchez
authors.forename: Yi-Ming
authors.initials: YM
authors.lastname: Chiang
authors.forename: Kenneth S
authors.initials: KS
authors.lastname: Bruno
authors.forename: Clay C C
authors.initials: CC
authors.lastname: Wang
mesh_terms: D001230:Aspergillus / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D053898:Biosynthetic Pathways / Q000235:genetics; D018390:Gene Targeting; D046908:Peptide Biosynthesis, Nucleic Acid-Independent / Q000235:genetics*; D010453:Peptide Synthases / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism
chemical_list: D010453:Peptide Synthases; C104385:non-ribosomal peptide synthase