title: Application of shRNA-containing herpes simplex virus type 1 (HSV-1)-based gene therapy for HSV-2-induced genital herpes.
abstract: HSV-1-based vectors have been widely used to achieve targeted delivery of genes into the nervous system. In the current study, we aim to use shRNA-containing HSV-1-based gene delivery system for the therapy of HSV-2 infection. Guinea pigs were infected intravaginally with HSV-2 and scored daily for 100 days for the severity of vaginal disease. HSV-2 shRNA-containing HSV-1 was applied intravaginally daily between 8 and 14 days after HSV-2 challenge. Delivery of HSV-2 shRNA-containing HSV-1 had no effect on the onset of disease and acute virus shedding in animals, but resulted in a significant reduction in both the cumulative recurrent lesion days and the number of days with recurrent disease. Around half of the animals in the HSV-2 shRNA group did not develop recurrent disease 100 days post HSV-2 infection. In conclusion, HSV-2 shRNA-containing HSV-1 particles are effective in reducing the recurrence of genital herpes caused by HSV-2.
authors.affiliation: Department of Obstetrics and Gynecology, Peking University Shenzhen Hospital, No. 1120, Lianhua Road, Futian District, Shenzhen, Guangdong 518036, China; Biomedical Research Institute, Shenzhen PKU-HKUST Medical Center, Shenzhen, Guangdong 518036, China.
authors.forename: Zhihong
authors.initials: Z
authors.lastname: Liu
authors.forename: Yang
authors.initials: Y
authors.lastname: Xiang
authors.forename: Zhun
authors.initials: Z
authors.lastname: Wei
authors.forename: Bo
authors.initials: B
authors.lastname: Yu
authors.forename: Yong
authors.initials: Y
authors.lastname: Shao
authors.forename: Jie
authors.initials: J
authors.lastname: Zhang
authors.forename: Hong
authors.initials: H
authors.lastname: Yang
authors.forename: Manmei
authors.initials: M
authors.lastname: Li
authors.forename: Ming
authors.initials: M
authors.lastname: Guan
authors.forename: Jun
authors.initials: J
authors.lastname: Wan
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D015316:Genetic Therapy / Q000379:methods*; D006168:Guinea Pigs; D006558:Herpes Genitalis / Q000473:pathology / Q000628:therapy* / Q000821:virology; D018259:Herpesvirus 1, Human / Q000235:genetics*; D018258:Herpesvirus 2, Human / Q000235:genetics*; D034741:RNA, Small Interfering / Q000235:genetics* / Q000627:therapeutic use*; D012720:Severity of Illness Index; D013997:Time Factors; D014621:Vagina / Q000821:virology; D017201:Virus Shedding
chemical_list: D034741:RNA, Small Interfering
keywords: Genital herpes; Herpes simplex virus type 1 (HSV-1) vector; Herpes simplex virus type 2 (HSV-2)