title: Construction of a chromosome-assigned, sequence-tagged linkage map for the radish, Raphanus sativus L. and QTL analysis of morphological traits.
abstract: The radish displays great morphological variation but the genetic factors underlying this variability are mostly unknown. To identify quantitative trait loci (QTLs) controlling radish morphological traits, we cultivated 94 F4 and F5 recombinant inbred lines derived from a cross between the rat-tail radish and the Japanese radish cultivar 'Harufuku' inbred lines. Eight morphological traits (ovule and seed numbers per silique, plant shape, pubescence and root formation) were measured for investigation. We constructed a map composed of 322 markers with a total length of 673.6 cM. The linkage groups were assigned to the radish chromosomes using disomic rape-radish chromosome-addition lines. On the map, eight and 10 QTLs were identified in 2008 and 2009, respectively. The chromosome-linkage group correspondence, the sequence-specific markers and the QTLs detected here will provide useful information for further genetic studies and for selection during radish breeding programs.
authors.affiliation: Graduate School of Life and Environmental Sciences, Kyoto Prefectural University , Seika, Kyoto 619-0244, Japan.
authors.forename: Tomoko
authors.initials: T
authors.lastname: Hashida
authors.forename: Ryoichi
authors.initials: R
authors.lastname: Nakatsuji
authors.forename: Holger
authors.initials: H
authors.lastname: Budahn
authors.forename: Otto
authors.initials: O
authors.lastname: Schrader
authors.forename: Herbert
authors.initials: H
authors.lastname: Peterka
authors.forename: Tatsuhito
authors.initials: T
authors.lastname: Fujimura
authors.forename: Nakao
authors.initials: N
authors.lastname: Kubo
authors.forename: Masashi
authors.initials: M
authors.lastname: Hirai
keywords: QTL; STS; chromosome assignment; linkage map; radish