title: Letter: Mass spectrometric approach of high pH- and copper-induced glutathione oxidation.
abstract: The interaction between copper ions and gamma-L-glutamyl-L-cysteinyl-glycine [glutathione (GSH)] molecules may lead to the formation of the physiologically occurring Cu[I)-[GSH]2 and Cu(II)-GSSG complexes. Since glutathione depletion in neurons and aberrant copper metabolism have been implicated in several neurodegenerative disorders, we studied here the interaction of GSH with copper ions (Cu2+) by electrospray ionization ion trap mass spectrometry (ESI-IT-MS). Besides, we extended this approach to pH in excess of 10 by adding ethanolamine to the solution being investigated. As a result, the ESI-IT-MS spectra revealed novel aspects regarding the speciation of copper-glutathione complex.
authors.forename: Gabi
authors.initials: G
authors.lastname: Drochioiu
authors.forename: Laura
authors.initials: L
authors.lastname: Ion
authors.forename: Catalina
authors.initials: C
authors.lastname: Ciobanu
authors.forename: Laura
authors.initials: L
authors.lastname: Habasescu
authors.forename: Ionel
authors.initials: I
authors.lastname: Mangalagiu
mesh_terms: D003300:Copper / Q000737:chemistry* / Q000378:metabolism; D019856:Ethanolamine / Q000737:chemistry; D019803:Glutathione Disulfide / Q000737:chemistry* / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D010084:Oxidation-Reduction; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
chemical_list: D019856:Ethanolamine; D003300:Copper; D019803:Glutathione Disulfide