title: A rapid and sensitive liquid chromatography-tandem mass spectrometric method for determination of actinoside E in rat plasma and application to a pharmacokinetic study.
abstract: A highly sensitive liquid chromatography-tandem mass spectrometric (LC-MS/MS) method was developed for the determination of actinoside E in rat plasma. The analytes were extracted by ethyl acetate and an analogue of actinoside F was used as the internal standard. The mobile phase consisted of methanol-water (50: 50, V/V) containing 0.1% formic acid was delivered at a flow rate of 0.3 mL·min(-1) to a Zorbax SB-C18 column (100 mm × 2.1 mm, 3.5 μm). The detection was performed by electrospray ionization mass spectrometry in the negative multiple reaction monitoring mode with a chromatograph run time of 3.0 min. Calibration curves of actinoside E were linear in the range of 0.5-2 500 ng·mL(-1). In this range, intra- and inter-day precision ranged from 1.7% to 7.5% and 2.0% to 8.9%, respectively. The accuracy ranged from 95.7% to 108.6%, and extraction recovery from 83.2% to 85.5%. This method was successfully applied to a pharmacokinetic study of actinoside E in rats after intravenous (5 mg·kg(-1)) and oral (100 mg·kg(-1)) administration, and the results showed that actinoside E was poorly absorbed with an absolute bioavailability being approximately 0.27%.
authors.affiliation: Department of Traditional Chinese Medicine, Changhai Hospital, Second Military Medical University, Shanghai 200433, China.
authors.forename: Li-Ping
authors.initials: LP
authors.lastname: Qu
authors.forename: Yong-Hua
authors.initials: YH
authors.lastname: Su
authors.forename: Guo-Yin
authors.initials: GY
authors.lastname: Zheng
authors.forename: Hai-Liang
authors.initials: HL
authors.lastname: Xin
authors.forename: Chang-Quan
authors.initials: CQ
authors.lastname: Ling
mesh_terms: D029042:Actinidia / Q000737:chemistry*; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006027:Glycosides / Q000097:blood* / Q000493:pharmacokinetics; D044949:Kaempferols / Q000097:blood* / Q000493:pharmacokinetics; D008297:Male; D010936:Plant Extracts / Q000097:blood* / Q000493:pharmacokinetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D006027:Glycosides; D044949:Kaempferols; D010936:Plant Extracts; C585869:actinoside E
keywords: Actinoside E; LC-MS/MS; Pharmacokinetics; Rat plasma