title: Transposition of IS elements induced by electroporation of suicide plasmid in Acidithiobacillus caldus.
abstract: Transposition insertional mutagenesis of the insertion sequences (IS elements) was discovered for the first time in Acidithiobacillus caldus (A. caldus), when A. caldus MTH-04 hsdM (type I restriction-modification system M-subunit) mutant was constructed by electroporation of a suicide plasmid. The IS element, specifically inserting into hsdM gene, was analyzed, identified, and named ISAtc2. The transposition frequency of ISAtc2 was ranged from 4% to 7%, and no reverse mutation occurred in the mutants after 50 generations of proliferation without selective pressure. These results revealed that transposition of IS elements on A. caldus chromosome could regulate the gene expression and metabolic pathways by gene inactivation, gene loss and gene acquisition. Therefore, the transposition of IS elements in A. caldus may be an important and unique regulation mechanism for adaptation to the living condition.
authors.affiliation: State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, China.
authors.forename: Linxu
authors.initials: L
authors.lastname: Chen
authors.forename: Jianqun
authors.initials: J
authors.lastname: Lin
authors.forename: Xiangmei
authors.initials: X
authors.lastname: Liu
authors.forename: Xin
authors.initials: X
authors.lastname: Pang
authors.forename: Huibin
authors.initials: H
authors.lastname: Lin
authors.forename: Jianqiang
authors.initials: J
authors.lastname: Lin
mesh_terms: D042763:Acidithiobacillus / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D015280:DNA Restriction-Modification Enzymes / Q000235:genetics; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000235:genetics; D018274:Electroporation; D005798:Genes, Bacterial; D041722:Genes, Transgenic, Suicide; D042822:Genomic Instability; D007218:Industrial Microbiology; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D010957:Plasmids / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D015280:DNA Restriction-Modification Enzymes; D004251:DNA Transposable Elements; D004269:DNA, Bacterial; C109829:HsdM protein, Bacteria
keywords: Acidithiobacillus caldus; Electroporation; IS elements; Suicide plasmid; Transposition mutagenesis