title: Evaluation of a commercially available carbohydrate deficient transferrin kit to detect beta-2-transferrin in cerebrospinal fluid using capillary electrophoresis.
abstract: OBJECTIVE
  The aim of this study was to evaluate the CEofix™ CDT Capillary Electrophoresis (CE) kit for the detection of β-2-tranferrin (β-2-Tf) in cerebrospinal fluid (CSF).
  
  
  METHODS
  Evaluation was performed according to CLSI EP5-A and EP12-A guidelines.
  
  
  RESULTS
  The method resolved β-2-Tf from other Tf isoforms. The C50 for β-Tf was determined to be 0.46 mg/L. Neither hemoglobin nor bilirubin co-migrated with β-2-Tf.
  
  
  CONCLUSIONS
  The CDT kit can be used for detecting β-2-Tf in CSF by CE.
authors.affiliation: Department of Pathology and Laboratory Medicine, McMaster University, Ontario, Canada; Hamilton Regional Medicine Program, Ontario, Canada. Electronic address: karina.capote@dynalifedx.com.
authors.forename: Karina
authors.initials: K
authors.lastname: Rodríguez-Capote
authors.forename: Joceline
authors.initials: J
authors.lastname: Turner
authors.forename: Joseph
authors.initials: J
authors.lastname: Macri
mesh_terms: D019075:Electrophoresis, Capillary / Q000379:methods*; D006801:Humans; D020033:Protein Isoforms / Q000134:cerebrospinal fluid; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity; D014168:Transferrin / Q000031:analogs & derivatives* / Q000134:cerebrospinal fluid
chemical_list: D020033:Protein Isoforms; D011933:Reagent Kits, Diagnostic; D014168:Transferrin; C056759:carbohydrate-deficient transferrin
keywords: CSF rhinorrhea; Capillary electrophoresis; Cerebrospinal fluid leakage; Desialylated transferrin; Tau-protein; β-2-transferrin