title: Simultaneous RP-HPLC-DAD separation, and determination of flavonoids and phenolic acids in Plantago L. species.
abstract: A rapid reversed-phase (RP) high-performance liquid chromatography method was developed and applied for simultaneous separation, and determination of flavonoids and phenolic acids in eight Plantago L. taxa (P. altissima L., P. argentea Chaix, P. coronopus L., P. holosteum Scop. ssp. depauperata Pilger, P. holosteum ssp. holosteum, P. holosteum ssp. scopulorum (Degen) Horvatić, P. lagopus L., and P. maritima L.) growing in Croatia. Chromatographic separation was carried out on Zorbax Eclipse XDB-C18 using gradient elution with a H2 O (pH 2.5, adjusted with CF3 COOH) and MeCN mixture at 30°. The contents of analyzed phenolic compounds (% of the dry weight of the leaves, dw) varied among examined species: rutin (max. 0.024%, P. argentea), hyperoside (max. 0.020%, P. lagopus), quercitrin (max. 0.013%, P. holosteum ssp. holosteum), quercetin (max. 0.028%, P. holosteum ssp. scopulorum), chlorogenic acid (max. 0.115%, P. lagopus), and caffeic acid (max. 0.046%, P. coronopus). Isoquercitrin was detected only in P. argentea (0.020%), while isochlorogenic acid content was below limit of quantification in all investigated species. Multivariate analyses (UPGMA and PCA) showed significant differences in contents of investigated polyphenolic compounds between different Plantago taxa. Accordingly, investigated substances might be employed as chemotaxonomic markers in the study of the complex genus Plantago.
authors.affiliation: Faculty of Pharmacy and Biochemistry, University of Zagreb, A. Kovačića 1, HR-10000 Zagreb. rjurisic@pharma.hr
authors.forename: Renata
authors.initials: R
authors.lastname: Jurišić Grubešić
authors.forename: Goran
authors.initials: G
authors.lastname: Srečnik
authors.forename: Dario
authors.initials: D
authors.lastname: Kremer
authors.forename: Jadranka
authors.initials: J
authors.lastname: Vuković Rodríguez
authors.forename: Toni
authors.initials: T
authors.lastname: Nikolić
authors.forename: Sanda
authors.initials: S
authors.lastname: Vladimir-Knežević
mesh_terms: D002851:Chromatography, High Pressure Liquid; D056148:Chromatography, Reverse-Phase; D005419:Flavonoids / Q000032:analysis* / Q000302:isolation & purification; D062385:Hydroxybenzoates / Q000032:analysis* / Q000302:isolation & purification; D015999:Multivariate Analysis; D018515:Plant Leaves / Q000737:chemistry; D010943:Plantago / Q000737:chemistry*; D025341:Principal Component Analysis
chemical_list: D005419:Flavonoids; D062385:Hydroxybenzoates; C017616:phenolic acid
keywords: Chemotaxonomy; Flavonoids; Phenolic acids; Plantago species