title: Assimilation of cellulose-derived carbon by microeukaryotes in oxic and anoxic slurries of an aerated soil.
abstract: Soil microeukaryotes may trophically benefit from plant biopolymers. However, carbon transfer from cellulose into soil microeukaryotes has not been demonstrated so far. Microeukaryotes assimilating cellulose-derived carbon in oxic and anoxic soil slurries were therefore examined by rRNA-based stable-isotope probing. Bacteriovorous flagellates and ciliates and, likely, mixotrophic algae and saprotrophic fungi incorporated carbon from supplemental [U-(13)C]cellulose under oxic conditions. A previous study using the same soil suggested that cellulolytic Bacteria assimilated (13)C of supplemental cellulose. Thus, it can be assumed that ciliates, cercozoa, and chrysophytes assimilated carbon by grazing upon and utilizing metabolic products of Bacteria that hydrolyzed cellulose in the soil slurries.
authors.affiliation: Helmholtz Centre of Environmental Research-UFZ, Department of Environmental Microbiology, Leipzig, Germany.
authors.forename: Antonis
authors.initials: A
authors.lastname: Chatzinotas
authors.forename: Stefanie
authors.initials: S
authors.lastname: Schellenberger
authors.forename: Karin
authors.initials: K
authors.lastname: Glaser
authors.forename: Steffen
authors.initials: S
authors.lastname: Kolb
mesh_terms: D000332:Aerobiosis; D000693:Anaerobiosis; D002244:Carbon / Q000378:metabolism*; D002482:Cellulose / Q000378:metabolism*; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D056890:Eukaryota / Q000378:metabolism*; D007553:Isotope Labeling; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology
chemical_list: D004275:DNA, Ribosomal; D002244:Carbon; D002482:Cellulose