title: Preparation and application of a molecularly imprinted polymer for determination of glibenclamide residues.
abstract: The performance of a molecularly imprinted polymer (MIP) as selective packing material for solid-phase extraction (SPE) of residual glibenclamide in an industrial process was investigated. MIP was prepared using 4-vinylpyridine as monomer, ethylenedimethacrylate as cross linker, 2,2'-azobis-2-methylpropionitrile as initiator and dimethyl formamide as porogen. Use of acetonitrile as a rebinding solvent allows good recognition of the glibenclamide template. It was found that this polymer can be used for determination of trace levels of glibenclamide with a recovery percentage that could reach 87.1 %. Furthermore, the synthesized MIP showed higher selectivity towards glibenclamide than other compounds such as glimepiride and metformin. The synthesized MIP enabled direct determination of the target contaminant after an enrichment step that allowed quantification of glibenclamide at a concentration as low as 0.016 mg L-1. Combination of high performance liquid chromatography with MIP-SPE could be successfully used for quality control of pharmaceuticals during the cleaning process in the production of dry drug forms.
authors.affiliation: Laboratoire de Chimie Analytique et d’Electrochimie, Université de Carthage, Institut National des Sciences Appliquées et de Technologie, Centre Urbain Nord B.P.N °676, Tunis Cedex, Tunisia.
authors.forename: Rym
authors.initials: R
authors.lastname: Lahsini
authors.forename: Mohamed R
authors.initials: MR
authors.lastname: Louhaichi
authors.forename: Nafaa
authors.initials: N
authors.lastname: Adhoum
authors.forename: Lotfi
authors.initials: L
authors.lastname: Monser
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003924:Diabetes Mellitus, Type 2 / Q000188:drug therapy; D004345:Drug Industry / Q000379:methods; D005905:Glyburide / Q000737:chemistry / Q000494:pharmacology; D006801:Humans; D007004:Hypoglycemic Agents / Q000737:chemistry / Q000494:pharmacology; D007220:Industrial Waste / Q000032:analysis / Q000517:prevention & control; D057230:Limit of Detection; D054802:Molecular Imprinting; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry; D011725:Pyridines / Q000737:chemistry; D052616:Solid Phase Extraction / Q000379:methods
chemical_list: D007004:Hypoglycemic Agents; D007220:Industrial Waste; D011108:Polymers; D011725:Pyridines; C029351:4-vinylpyridine; D005905:Glyburide