title: Proteomic analysis of a high aluminum tolerant yeast Rhodotorula taiwanensis RS1 in response to aluminum stress.
abstract: Rhodotorula taiwanensis RS1 is a high-aluminum (Al)-tolerant yeast that can survive in Al concentrations up to 200mM. The mechanisms for the high Al tolerance of R. taiwanensis RS1 are not well understood. To investigate the molecular mechanisms underlying Al tolerance and toxicity in R. taiwanensis RS1, Al toxicity-induced changes in the total soluble protein profile were analyzed using two-dimensional gel electrophoresis (2-DE) coupled with mass spectrometry. A total of 33 differentially expressed proteins responding to Al stress were identified from approximately 850 reproducibly detected proteins. Among them, the abundance of 29 proteins decreased and 4 increased. In the presence of 100mM Al, the abundance of proteins involved in DNA transcription, protein translation, DNA defense, Golgi functions and glucose metabolism was decreased. By contrast, Al treatment led to increased abundance of malate dehydrogenase, which correlated with increased malate dehydrogenase activity and the accumulation of intracellular citrate, suggesting that Al-induced intracellular citrate could play an important role in detoxification of Al in R. taiwanensis RS1.
authors.affiliation: State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China; University of Chinese Academy of Sciences, Beijing 100049, China. Electronic address: cwang@iue.ac.cn.
authors.forename: Chao
authors.initials: C
authors.lastname: Wang
authors.forename: Chang Yi
authors.initials: CY
authors.lastname: Wang
authors.forename: Xue Qiang
authors.initials: XQ
authors.lastname: Zhao
authors.forename: Rong Fu
authors.initials: RF
authors.lastname: Chen
authors.forename: Ping
authors.initials: P
authors.lastname: Lan
authors.forename: Ren Fang
authors.initials: RF
authors.lastname: Shen
mesh_terms: D000535:Aluminum / Q000378:metabolism / Q000494:pharmacology*; D019343:Citric Acid / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects*; D008291:Malate Dehydrogenase / Q000235:genetics / Q000378:metabolism*; D013058:Mass Spectrometry; D053858:Metabolic Networks and Pathways / Q000187:drug effects; D040901:Proteomics; D012248:Rhodotorula / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D012988:Soil Microbiology
chemical_list: D005656:Fungal Proteins; D019343:Citric Acid; D000535:Aluminum; D008291:Malate Dehydrogenase
keywords: 2-DE; Aluminum; Citrate; Malate dehydrogenase; Rhodotorula taiwanensis