title: Effect of the combined probiotics with aflatoxin B₁-degrading enzyme on aflatoxin detoxification, broiler production performance and hepatic enzyme gene expression.
abstract: In order to degrade aflatoxin B₁ (AFB₁), AFB₁-degrading microbes (probiotics) such as Lactobacillus casei, Bacillus subtilis and Pichia anomala, and the AFB₁-degrading enzyme from Aspergillus oryzae were selected and combined to make feed additive. Seventy-five 43-day-old male Arbor Acres broilers were randomly divided into 5 groups, 15 broilers for each group. The broilers were given with 5 kinds of diets such as the basal diet, 400 μg/kg AFB₁ supplement without feed additive, and 200, 400, 800 μg/kg AFB₁ supplement with 0.15% feed additive. The feeding experimental period was 30 d, which was used to determine production performance of broilers. In addition, serum, liver and chest muscle were selected for measuring AFB₁ residues, gene expressions, microscopic and antioxidant analyses. The results showed that adding 0.15% feed additive in broiler diets could significantly relieve the negative effect of AFB₁ on chicken's production performance and nutrient metabolic rates (P<0.05). It could also improve AFB₁ metabolism, hepatic cell structure, antioxidant activity, and many hepatic enzyme gene expressions involved in oxidoreductase, apoptosis, cell growth, immune system and metabolic process (P<0.05). It could be concluded that the feed additive was able to degrade AFB₁ and improve animal production.
authors.affiliation: College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, China; Henan Husbandry Breau, Zhengzhou 450002, China.
authors.forename: Rui-yu
authors.initials: RY
authors.lastname: Zuo
authors.forename: Juan
authors.initials: J
authors.lastname: Chang
authors.forename: Qing-qiang
authors.initials: QQ
authors.lastname: Yin
authors.forename: Ping
authors.initials: P
authors.lastname: Wang
authors.forename: Yu-rong
authors.initials: YR
authors.lastname: Yang
authors.forename: Xiao
authors.initials: X
authors.lastname: Wang
authors.forename: Guo-qiang
authors.initials: GQ
authors.lastname: Wang
authors.forename: Qiu-hong
authors.initials: QH
authors.lastname: Zheng
mesh_terms: D016604:Aflatoxin B1 / Q000037:antagonists & inhibitors* / Q000378:metabolism / Q000633:toxicity; D000449:Aldehyde Reductase / Q000008:administration & dosage / Q000378:metabolism / Q000627:therapeutic use*; D000818:Animals; D017849:Animals, Inbred Strains; D000992:Antitoxins / Q000008:administration & dosage / Q000378:metabolism / Q000627:therapeutic use*; D001231:Aspergillus flavus / Q000201:enzymology / Q000254:growth & development; D030161:Avian Proteins / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D001412:Bacillus subtilis / Q000254:growth & development; D002273:Carcinogens / Q000037:antagonists & inhibitors / Q000378:metabolism / Q000633:toxicity; D002645:Chickens; D002681:China; D002149:Energy Intake; D005506:Food Contamination; D005517:Foodborne Diseases / Q000209:etiology / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control; D005656:Fungal Proteins / Q000378:metabolism / Q000627:therapeutic use*; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D007780:Lacticaseibacillus casei / Q000254:growth & development; D008099:Liver / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D008297:Male; D010843:Pichia / Q000254:growth & development; D019936:Probiotics / Q000008:administration & dosage / Q000378:metabolism / Q000627:therapeutic use*; D015430:Weight Gain
chemical_list: D000992:Antitoxins; D030161:Avian Proteins; D002273:Carcinogens; D005656:Fungal Proteins; D016604:Aflatoxin B1; C082765:aflatoxin B1 aldehyde reductase; D000449:Aldehyde Reductase
keywords: AFB(1)-degrading enzyme; Aflatoxin B(1); Broiler production performance; Gene expressions; Probiotics