title: [Accessing the features of surface neuraminidase (N1) of influenza A virus presenting on the platforms for anti-NA Abs screening].
abstract: OBJECTIVE
  To understand if the Neuraminidase (N1) of Influenza A virus at the surface of yeast-displaying system, eukaryotic expression system and the infected cells could be used for anti-NA Abs screening, their activities and bindings to five candidate Abs were assayed.
  
  
  METHODS
  The surface NA expression was obtained by transfecting by recombinant NA constructors with specific tag-labels or live virus infection. The functional activity was measured by the fluorescent assay. Their bindings to the Abs were detected by flow cytometry.
  
  
  RESULTS
  The surface NAs presenting on the yeast-displaying system and eukaryotic expression system exhibited functional NA activities as the NA at the surface of virus-infected cells which showed affinities to Ab1, 4, and 5. The same bindings to Abl and 5 were found in the surface NA expressed by eukaryotic expression system while minor binding was observed in the yeast displayed-NA.
  
  
  CONCLUSIONS
  The epitopes of yeast-displayed NA may be different from the NAs present at eukaryotic expression system and the infected cells which more likely suitable for the screening of anti-NA Abs.
authors.affiliation: Children's Hospital of Changqing Medical University, China.
authors.forename: Lan
authors.initials: L
authors.lastname: Huang
authors.forename: Kun
authors.initials: K
authors.lastname: Qin
authors.forename: Jian-fang
authors.initials: JF
authors.lastname: Zhou
authors.forename: Yue-long
authors.initials: YL
authors.lastname: Shu
authors.forename: Hong
authors.initials: H
authors.lastname: Wei
mesh_terms: D000906:Antibodies / Q000276:immunology*; D000954:Antigens, Surface / Q000235:genetics* / Q000276:immunology*; D002460:Cell Line; D057809:HEK293 Cells; D006801:Humans; D009980:Influenza A virus / Q000235:genetics* / Q000276:immunology*; D009439:Neuraminidase / Q000235:genetics* / Q000276:immunology*; D011485:Protein Binding; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology
chemical_list: D000906:Antibodies; D000954:Antigens, Surface; D011994:Recombinant Proteins; D009439:Neuraminidase