title: Genetic characterization of a rare bovine-like human VP4 mono-reassortant G6P[8] rotavirus strain detected from an infant in Bangladesh.
abstract: During an ongoing diarrhea etiology surveillance in Mirzapur, Bangladesh, a rare human G6P[8] RVA strain (RVA/Human-wt/BGD/KH2288/2011/G6P[8]) was detected in a stool sample of a 7-month-old infant with acute diarrhea. Complete genotype analyses revealed that KH2288 possessed the G6-P[8]-I2-R2-C2-M2-A11-N2-T6-E2-H3 genotype constellation. Sequence analysis of the VP7 gene revealed a close phylogenetic relationship with bovine G6 strains from India, whereas, the VP4 gene segment was nearly identical to typical human P[8] strain circulating in Bangladesh and the rest of the world. Phylogenetic analysis of the remaining nine gene segments revealed a close relatedness to either animal or animal derived human RVA strain. We speculated that, strain KH2288 was a monoreassortant between a human RVA strain and a RVA strain typically infecting member of the Artiodactyla, such as cattle, goat or sheep. To our knowledge, this is the first complete genotyping report of a naturally occurring G6P[8] RVA strain, worldwide.
authors.affiliation: Virology Laboratory, International Centre for Diarrhoeal Disease Research, Bangladesh (icddr,b). Electronic address: hassan_afrad@icddrb.org.
authors.forename: Mokibul Hassan
authors.initials: MH
authors.lastname: Afrad
authors.forename: Jelle
authors.initials: J
authors.lastname: Matthijnssens
authors.forename: Sayra
authors.initials: S
authors.lastname: Moni
authors.forename: Farzana
authors.initials: F
authors.lastname: Kabir
authors.forename: Adnin
authors.initials: A
authors.lastname: Ashrafi
authors.forename: Mohammed Ziaur
authors.initials: MZ
authors.lastname: Rahman
authors.forename: Abu S G
authors.initials: AS
authors.lastname: Faruque
authors.forename: Tasnim
authors.initials: T
authors.lastname: Azim
authors.forename: Mustafizur
authors.initials: M
authors.lastname: Rahman
mesh_terms: D036022:Capsid Proteins / Q000235:genetics*; D005243:Feces / Q000821:virology; D006801:Humans; D007223:Infant; D008297:Male; D010802:Phylogeny; D016865:Reassortant Viruses / Q000235:genetics; D012401:Rotavirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D012400:Rotavirus Infections / Q000821:virology*
chemical_list: D036022:Capsid Proteins; C060339:VP4 protein, Rotavirus
keywords: Bangladesh; Diarrhea; G6P[8]; Interspecies transmission; Rotavirus