title: Preparation and chromatographic evaluation of a novel phosphate ester-bonded stationary phase with complexation and hydrophobic interactions retention mechanism.
abstract: The present study described the preparation of a novel phosphate ester-bonded silica (PES) stationary phase based on "thiol-ene" click chemistry. The composition of the surface grafts of PES stationary phase was determined by elemental analysis and solid state (31)P MAS NMR. Due to its hydrophilic phosphate-ester groups and short hydrophobic alkyl chains, the PES stationary phase exhibited dual retention mechanism via complexation and hydrophobic interactions with phenols. Benefiting from this special interaction mechanism, the newly synthesized PES stationary phase showed better selectivity in the separation of phenols compared to commercial octadecylsilyl-bonded silica (C18) columns. Furthermore, the separations of 10 nucleosides and nucleobases on the PES stationary phase were achieved in both reversed-phase liquid chromatography (RPLC) mode and hydrophilic interaction liquid chromatography (HILIC) mode.
authors.affiliation: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), Department of Chemistry, Wuhan University, Wuhan 430072, China.
authors.forename: Xiao-Dong
authors.initials: XD
authors.lastname: Cheng
authors.forename: Xi-Tian
authors.initials: XT
authors.lastname: Peng
authors.forename: Qiong-Wei
authors.initials: QW
authors.lastname: Yu
authors.forename: Bi-Feng
authors.initials: BF
authors.lastname: Yuan
authors.forename: Yu-Qi
authors.initials: YQ
authors.lastname: Feng
mesh_terms: D056148:Chromatography, Reverse-Phase / Q000379:methods*; D057930:Click Chemistry; D057927:Hydrophobic and Hydrophilic Interactions; D010636:Phenols / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry*
chemical_list: D010636:Phenols; D012822:Silicon Dioxide
keywords: Complexation; Phenols; Stationary phase; “Thiol-ene” click chemistry