title: Silencing of GATA6 suppresses SW1990 pancreatic cancer cell growth in vitro and up-regulates reactive oxygen species.
abstract: OBJECTIVE
  Pancreatic cancer has the worst prognosis of any gastrointestinal cancer with a mortality rate approaching its incidence. Previous studies have indicated that GATA6 plays a key role in organ development and function, and that abnormal expression of GATA6 may induce tumorigenesis. Meanwhile, it has been reported that generation of reactive oxygen species contributes to carcinogenesis. In this study, we set out to study the role of GATA6 expression on proliferation and apoptosis of pancreatic cancer cells and the role of reactive oxygen species.
  
  
  METHODS
  Four target miRNA sequences against GATA6 mRNA were synthesized and used to transfect SW1990 cells. Then, GATA6 expression in SW1990 cells was examined by western blot and quantative real-time polymerase chain reaction. Cell proliferation was examined by WST-8 and colony formation assay. Cell cycle progression and apoptosis were measured by flow cytometry. We also measured the generation of reactive oxygen species by immunofluorescence and flow cytometry.
  
  
  RESULTS
  RNA interference against GATA6 successfully inhibited mRNA and protein expression of GATA6 in the SW1990 pancreatic cancer cell line. Silencing of GATA6 by RNA interference inhibited cell proliferation and increased apoptosis of SW1990, and enhanced the expression of reactive oxygen species.
  
  
  CONCLUSIONS
  These results suggest that the RNA interference approach against GATA6 may be an effective therapeutic approach for treatment of pancreatic cancer.
authors.affiliation: Department of Gastroenterology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, No. 107 Yanjiang West Road, Guangzhou, 510120, Guangdong, People's Republic of China. wenbochen2012@163.com
authors.forename: Wen-Bo
authors.initials: WB
authors.lastname: Chen
authors.forename: Feng-Ting
authors.initials: FT
authors.lastname: Huang
authors.forename: Yan-Yan
authors.initials: YY
authors.lastname: Zhuang
authors.forename: Jian
authors.initials: J
authors.lastname: Tang
authors.forename: Xiao-Hong
authors.initials: XH
authors.lastname: Zhuang
authors.forename: Wen-Jie
authors.initials: WJ
authors.lastname: Cheng
authors.forename: Zhi-Qiang
authors.initials: ZQ
authors.lastname: Gu
authors.forename: Shi-Neng
authors.initials: SN
authors.lastname: Zhang
mesh_terms: D000230:Adenocarcinoma / Q000378:metabolism / Q000628:therapy*; D017209:Apoptosis / Q000187:drug effects; D016022:Case-Control Studies; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D005260:Female; D050993:GATA6 Transcription Factor / Q000378:metabolism*; D020868:Gene Silencing; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000494:pharmacology / Q000627:therapeutic use*; D008875:Middle Aged; D010190:Pancreatic Neoplasms / Q000378:metabolism / Q000628:therapy*; D010195:Pancreatitis / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D050993:GATA6 Transcription Factor; C494759:GATA6 protein, human; D035683:MicroRNAs; D017382:Reactive Oxygen Species