title: Resonance light scattering technique as a new tool to determine the binding mode of anticancer drug oridonin to DNA.
abstract: In this study, a sensitive and reliable assay has been developed to determine the binding mode of oridonin to DNA in vitro using resonance light scattering (RLS) technique. The binding mode of ORI with DNA has been confirmed by measuring the changes of RLS intensity under different pH and ionic strength. It is the ORI that is intercalated into the double helix DNA to destroy its template function and inhibit the synthesis of DNA. Moreover, the RLS assay result was validated by seasoned vitro methods. The proposed RLS assay is not only an unprecedented one for primarily determine the binding mode of oridonin to DNA, but also a simple, sensitive, objective and straightforward method for the detection of ORI.
authors.affiliation: Department of Chemistry, Shantou University, Shantou 515063, China. kqlu@stu.edu.cn
authors.forename: Zhanguang
authors.initials: Z
authors.lastname: Chen
authors.forename: Zhen
authors.initials: Z
authors.lastname: Wang
authors.forename: Junhui
authors.initials: J
authors.lastname: Chen
authors.forename: Xi
authors.initials: X
authors.lastname: Chen
authors.forename: Jiahui
authors.initials: J
authors.lastname: Wu
authors.forename: Yanyu
authors.initials: Y
authors.lastname: Wu
authors.forename: Jieyao
authors.initials: J
authors.lastname: Liang
mesh_terms: D000970:Antineoplastic Agents / Q000378:metabolism*; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D045786:Diterpenes, Kaurane / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D008027:Light; D008958:Models, Molecular; D009690:Nucleic Acid Conformation; D009994:Osmolar Concentration; D012542:Scattering, Radiation
chemical_list: D000970:Antineoplastic Agents; D045786:Diterpenes, Kaurane; C011959:oridonin; D004247:DNA
keywords: AFM; Binding mode; DNA; Intracellular target; ORI; Oridonin; RLS; Resonance light scattering; SEM; atom force microscopy; oridonin; resonance light scattering; scan electronic microscopy