title: Immunoblotting for active caspase-1.
abstract: Immunoblotting for caspase-1 is the gold-standard method of detecting inflammasome activation. In contrast to IL-1β-based readouts, it can be used in an experimental setup independent of de novo gene expression. Here, we present protocols for the preparation and precipitation of supernatant samples containing activated caspase-1 as well as protocols for polyacrylamide gel electrophoresis (PAGE) and protein immunoblotting.
authors.affiliation: Institute for Clinical Chemistry and Pharmacology, Unit for Clinical Biochemistry, University Hospital, University of Bonn, Bonn, Germany.
authors.forename: Christopher
authors.initials: C
authors.lastname: Jakobs
authors.forename: Eva
authors.initials: E
authors.lastname: Bartok
authors.forename: Andrej
authors.initials: A
authors.lastname: Kubarenko
authors.forename: Franz
authors.initials: F
authors.lastname: Bauernfeind
authors.forename: Veit
authors.initials: V
authors.lastname: Hornung
mesh_terms: D000818:Animals; D020170:Caspase 1 / Q000378:metabolism*; D015151:Immunoblotting / Q000379:methods; D008264:Macrophages / Q000378:metabolism; D051379:Mice
chemical_list: D020170:Caspase 1