title: Anti-neutrophilic inflammatory steroidal glycosides from Solanum torvum.
abstract: Torvpregnanosides A and B, two pregnane glycosides, and torvoside Q, a 23-keto-spirostanol glycoside, along with twelve known steroidal saponins were isolated from aerial parts of Solanum torvum. Of the latter, four of the 23-hydroxy-spirostanol glycosides, and, a yamogenin glycoside, were in this plant discovered. All structures were identified from spectroscopic data, and all the compounds were tested for in vitro anti-neutrophilic inflammatory activity. Two compounds showed selective inhibition against elastase release and superoxide anion generation, respectively, by human neutrophils with IC50 values of 5.66 and 3.59 μM, while two others inhibited both inflammatory mediators with IC50 values of 0.66-3.49 μM. Structure-activity relationships are discussed.
authors.affiliation: School of Chinese Medicine, College of Chinese Medicine, China Medical University, Taichung 40402, Taiwan; Chinese Medicine Research and Development Center, China Medical University Hospital, Taichung 40402, Taiwan.
authors.forename: Chia-Lin
authors.initials: CL
authors.lastname: Lee
authors.forename: Tsong-Long
authors.initials: TL
authors.lastname: Hwang
authors.forename: Wan-Jung
authors.initials: WJ
authors.lastname: He
authors.forename: Yi-Hong
authors.initials: YH
authors.lastname: Tsai
authors.forename: Chiao-Ting
authors.initials: CT
authors.lastname: Yen
authors.forename: Hsin-Fu
authors.initials: HF
authors.lastname: Yen
authors.forename: Chao-Jung
authors.initials: CJ
authors.lastname: Chen
authors.forename: Wei-Yi
authors.initials: WY
authors.lastname: Chang
authors.forename: Yang-Chang
authors.initials: YC
authors.lastname: Wu
mesh_terms: D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D006027:Glycosides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D018836:Inflammation Mediators / Q000378:metabolism; D020128:Inhibitory Concentration 50; D015394:Molecular Structure; D009504:Neutrophils / Q000378:metabolism*; D010196:Pancreatic Elastase / Q000037:antagonists & inhibitors; D010840:Phytosterols / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D035261:Plant Components, Aerial; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D011278:Pregnanes / Q000737:chemistry / Q000302:isolation & purification; D012503:Saponins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D032322:Solanum / Q000737:chemistry*; D013150:Spirostans / Q000737:chemistry / Q000302:isolation & purification; D013481:Superoxides / Q000378:metabolism
chemical_list: D000893:Anti-Inflammatory Agents; D006027:Glycosides; D018836:Inflammation Mediators; D010840:Phytosterols; D010936:Plant Extracts; D011278:Pregnanes; D012503:Saponins; D013150:Spirostans; C574437:pregnane glycoside; D013481:Superoxides; D010196:Pancreatic Elastase
keywords: Anti-neutrophilic inflammation; Solanaceae; Solanum torvum; Steroidal glycoside