title: Piezomicrogravimetric and impedimetric oligonucleotide biosensors using conducting polymers of biotinylated bis(2,2'-bithien-5-yl)methane as recognition units.
abstract: A new conducting polymer of biotinylated bis(2,2'-bithien-5-yl)methane was prepared and applied as the recognition unit of two different biosensors for selective oligonucleotide determination using either electrochemical impedance spectroscopy (EIS) or piezoelectric microgravimetry (PM) for label-free analytical signal transduction. For preparation of this unit, first, a biotinylated bis(2,2'-bithien-5-yl)methane functional monomer was designed and synthesized. Then, this monomer was potentiodynamically polymerized to form films on the surface of a glassy carbon electrode (GCE) and a Au electrode of a quartz crystal resonator (QCR) for the EIS and PM transduction, respectively. On top of these films, neutravidin was irreversibly immobilized by complexing the biotin moieties of the polymer. Finally, recognizing biotinylated oligonucleotide was attached by complexing the surface-immobilized neutravidin. This layer-by-layer assembling of the poly(thiophene-biotin)-neutravidin-(biotin-oligonucleotide) recognition film served to determine the target oligonucleotide via complementary nucleobase pairing. Under optimized determination conditions, the target oligonucleotide limit of detection (LOD) was 0.5 pM and 50 nM for the EIS and PM transduction, respectively. The sensor response to the target oligonucleotide was linear with respect to logarithm of the target oligonucleotide concentration in a wide range of 0.5 pM to 30 μM and with respect to its concentration in the range of 50 to 600 nM for the EIS and PM transduction, respectively. The biosensors were appreciably selective with respect to the nucleobase mismatched oligonucleotides.
authors.affiliation: Department of Physical Chemistry of Supramolecular Complexes, Institute of Physical Chemistry, Polish Academy of Sciences, Warsaw, Poland.
authors.forename: Marta
authors.initials: M
authors.lastname: Sosnowska
authors.forename: Piotr
authors.initials: P
authors.lastname: Pieta
authors.forename: Piyush S
authors.initials: PS
authors.lastname: Sharma
authors.forename: Raghu
authors.initials: R
authors.lastname: Chitta
authors.forename: B K C
authors.initials: BK
authors.lastname: Chandra
authors.forename: Venugopal
authors.initials: V
authors.lastname: Bandi
authors.forename: Francis
authors.initials: F
authors.lastname: D'Souza
authors.forename: Wlodzimierz
authors.initials: W
authors.lastname: Kutner
mesh_terms: D001360:Avidin / Q000737:chemistry / Q000378:metabolism; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D019921:Biotinylation; D004553:Electric Conductivity; D017097:Electric Impedance; D004566:Electrodes; D055767:Immobilized Proteins / Q000737:chemistry / Q000378:metabolism; D008697:Methane / Q000737:chemistry*; D008958:Models, Molecular; D008968:Molecular Conformation; D009841:Oligonucleotides / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D011108:Polymers / Q000737:chemistry*; D013876:Thiophenes / Q000737:chemistry*; D014893:Weightlessness
chemical_list: D055767:Immobilized Proteins; D009841:Oligonucleotides; D011108:Polymers; D013876:Thiophenes; C586225:bis(2,2'-bithien-5-yl)methane; C093483:neutravidin; D001360:Avidin; D008697:Methane