title: Performance of different Trypanosoma cruzi antigens in the diagnosis of Chagas disease in patients with American cutaneous leishmaniasis from a co-endemic region in Argentina.
abstract: OBJECTIVE
  To determine the ability of recombinant antigens to detect cases of infection with Trypanosoma cruzi among cases of infection with Leishmania spp. by serological methods.
  
  
  METHODS
  Sera from 41 patients infected with Leishmania spp. were evaluated with ELISA using single (FRA, CP1 and TSSAVI) or pooled (commercial Rec-ELISA) recombinant proteins or homogenate antigens (commercial H-ELISA). As there is no gold standard antigen to discriminate Chagas disease from leishmaniasis, the correlation of results between defined antigens and the homogenate was made with Kappa Index (KI), the level of correlation considered being used as a criterion of specificity.
  
  
  RESULTS
  Single recombinant antigens and Rec-ELISA showed good correlation (KI > 0.8). A low correlation (KI < 0.66) was observed between the results from single recombinant antigens or the commercial recombinant kit and H-ELISA.
  
  
  CONCLUSIONS
  The highly correlated results between T. cruzi single or pooled recombinant proteins are indicative of the usefulness of recombinant antigens for Chagas diagnosis. Our results also indicate that in the city of Oran in Argentina, between 12% and 17% of patients with leishmaniasis are also infected with Chagas disease. The high KI values between TSSAVI and the other recombinant proteins suggest that in these patients, the infection may be caused by T. cruzi II and/or V and/or VI lineages.
authors.affiliation: Cátedra de Química Biológica, Facultad de Ciencias Naturales, Universidad Nacional de Salta, Salta, Argentina.|Instituto de Investigaciones de Enfermedades Tropicales, Sede Regional Orán, Universidad Nacional de Salta, Salta, Argentina.|Instituto de Patología Experimental, Facultad de Ciencias de la Salud, Universidad Nacional de Salta, Salta, Argentina.
authors.forename: A F
authors.initials: AF
authors.lastname: Vega Benedetti
authors.affiliation: Cátedra de Química Biológica, Facultad de Ciencias Naturales, Universidad Nacional de Salta, Salta, Argentina.|Instituto de Investigaciones de Enfermedades Tropicales, Sede Regional Orán, Universidad Nacional de Salta, Salta, Argentina.
authors.forename: Rubén O
authors.initials: RO
authors.lastname: Cimino
authors.affiliation: Instituto de Investigaciones de Enfermedades Tropicales, Sede Regional Orán, Universidad Nacional de Salta, Salta, Argentina.
authors.forename: Pamela S
authors.initials: PS
authors.lastname: Cajal
authors.affiliation: Instituto de Investigaciones de Enfermedades Tropicales, Sede Regional Orán, Universidad Nacional de Salta, Salta, Argentina.
authors.forename: Marisa Del Valle
authors.initials: MDV
authors.lastname: Juarez
authors.affiliation: Instituto de Investigaciones de Enfermedades Tropicales, Sede Regional Orán, Universidad Nacional de Salta, Salta, Argentina.
authors.forename: Carlos A
authors.initials: CA
authors.lastname: Villalpando
authors.affiliation: Instituto de Investigaciones de Enfermedades Tropicales, Sede Regional Orán, Universidad Nacional de Salta, Salta, Argentina.|Consejo Nacional de Investigaciones Científicas y Técnicas, Buenos Aires, Argentina.
authors.forename: José F
authors.initials: JF
authors.lastname: Gil
authors.affiliation: Laboratorio de Tecnología Inmunológica, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, Santa Fe, Argentina.|Consejo Nacional de Investigaciones Científicas y Técnicas, Buenos Aires, Argentina.
authors.forename: Iván S
authors.initials: IS
authors.lastname: Marcipar
authors.affiliation: Instituto de Investigaciones de Enfermedades Tropicales, Sede Regional Orán, Universidad Nacional de Salta, Salta, Argentina.|Instituto de Patología Experimental, Facultad de Ciencias de la Salud, Universidad Nacional de Salta, Salta, Argentina.|Consejo Nacional de Investigaciones Científicas y Técnicas, Buenos Aires, Argentina.
authors.forename: Alejandro J
authors.initials: AJ
authors.lastname: Krolewiecki
authors.affiliation: Cátedra de Química Biológica, Facultad de Ciencias Naturales, Universidad Nacional de Salta, Salta, Argentina.|Instituto de Investigaciones de Enfermedades Tropicales, Sede Regional Orán, Universidad Nacional de Salta, Salta, Argentina.
authors.forename: Julio R
authors.initials: JR
authors.lastname: Nasser
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000953:Antigens, Protozoan / Q000097:blood*; D001118:Argentina / Q000453:epidemiology; D014355:Chagas Disease / Q000097:blood* / Q000175:diagnosis / Q000453:epidemiology / Q000276:immunology; D002648:Child; D015897:Comorbidity; D003429:Cross Reactions / Q000276:immunology; D019353:Endemic Diseases; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007891:Leishmania / Q000276:immunology / Q000302:isolation & purification; D016773:Leishmaniasis, Cutaneous / Q000097:blood* / Q000175:diagnosis / Q000453:epidemiology / Q000276:immunology; D008297:Male; D008875:Middle Aged; D012680:Sensitivity and Specificity; D012698:Serologic Tests / Q000379:methods; D014349:Trypanosoma cruzi / Q000276:immunology* / Q000302:isolation & purification; D055815:Young Adult
chemical_list: D000953:Antigens, Protozoan
keywords: Trypanosoma cruzi; leishmaniasis; recombinant antigen