title: The Heterologous Expression of the Chrysanthemum R2R3-MYB Transcription Factor CmMYB1 Alters Lignin Composition and Represses Flavonoid Synthesis in Arabidopsis thaliana.
abstract: Plant R2R3-MYB transcription factor genes are widely distributed in higher plants and play important roles in the regulation of many secondary metabolites at the transcriptional level. In this study, a chrysanthemum subgroup 4 R2R3-MYB transcription factor gene, designated CmMYB1, was isolated through screening chrysanthemum EST (expressed sequence tag) libraries and using rapid application of cDNA ends (RACE) methods and functionally characterized. CmMYB1 is expressed in the root, stem, leaf and flowers, but most strongly in the stem and most weakly in the root. Its heterologous expression in Arabidopsis thaliana reduced the lignin content and altered the lignin composition. The heterologous expression also repressed the flavonoids content in A. thaliana. Together, these results suggested that CmMYB1 is a negative regulator of genes involved in the lignin pathway and flavonoid pathway, it may be a promising gene for controlling lignin and flavonoids profiles in plants.
authors.affiliation: College of Horticulture, Nanjing Agricultural University, Nanjing, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Zhu
authors.forename: Hong
authors.initials: H
authors.lastname: Shan
authors.forename: Sumei
authors.initials: S
authors.lastname: Chen
authors.forename: Jiafu
authors.initials: J
authors.lastname: Jiang
authors.forename: Chunsun
authors.initials: C
authors.lastname: Gu
authors.forename: Guoqin
authors.initials: G
authors.lastname: Zhou
authors.forename: Yu
authors.initials: Y
authors.lastname: Chen
authors.forename: Aiping
authors.initials: A
authors.lastname: Song
authors.forename: Fadi
authors.initials: F
authors.lastname: Chen
mesh_terms: D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism; D031202:Chrysanthemum / Q000235:genetics / Q000378:metabolism*; D003001:Cloning, Molecular / Q000379:methods*; D020224:Expressed Sequence Tags; D005419:Flavonoids / Q000096:biosynthesis; D035264:Flowers / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D008031:Lignin / Q000378:metabolism; D010802:Phylogeny; D018515:Plant Leaves / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D018517:Plant Roots / Q000378:metabolism; D018547:Plant Stems / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000378:metabolism; D017422:Sequence Analysis, DNA / Q000379:methods*; D014018:Tissue Distribution; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism*
chemical_list: D005419:Flavonoids; D010940:Plant Proteins; D014157:Transcription Factors; D008031:Lignin