title: Comparative analysis of dynamic proteomic profiles between in vivo and in vitro produced mouse embryos during postimplantation period.
abstract: Assisted reproductive technology (ART) increasingly is associated with long-term side-effects on postnatal development and behaviors. High-throughput gene expression analysis has been extensively used to explore mechanisms responsible for these disorders. Our study, for the first time, provides a comparative proteomic analysis between embryos after in vivo fertilization and development (IVO, control) and in vitro fertilization and culture (IVP). By comparing the dynamic proteome during the postimplantation period, we identified 300 and 262 differentially expressed proteins (DEPs) between IVO and IVP embryos at embryonic day 7.5 (E7.5) and E10.5, respectively. Bioinformatic analysis showed many DEPs functionally associated with post-transcriptional, translational, and post-translational regulation, and these observations were consistent with correlation analysis between mRNA and protein abundance. In addition to altered gene expression due to IVP procedures, our findings suggest that aberrant processes at these various levels also contributed to proteomic alterations. In addition, numerous DEPs were involved in energy and amino acid metabolism, as well as neural and sensory development. These DEPs are potential candidates for further exploring the mechanism(s) of ART-induced intrauterine growth restriction and neurodevelopmental disorders. Moreover, significant enrichment of DEPs in pathways of neurodegenerative diseases implies the potentially increased susceptibility of ART offspring to these conditions as adults.
authors.affiliation: Ministry of Agriculture Key Laboratory of Animal Genetics, Breeding and Reproduction, National Engineering Laboratory for Animal Breeding, College of Animal Sciences and Technology, China Agricultural University, No. 2 Yuanmingyuan Xi Lu, Haidian, Beijing 100193, China.
authors.forename: Jingzhou
authors.initials: J
authors.lastname: Nie
authors.forename: Lei
authors.initials: L
authors.lastname: An
authors.forename: Kai
authors.initials: K
authors.lastname: Miao
authors.forename: Zhuocheng
authors.initials: Z
authors.lastname: Hou
authors.forename: Yong
authors.initials: Y
authors.lastname: Yu
authors.forename: Kun
authors.initials: K
authors.lastname: Tan
authors.forename: Linlin
authors.initials: L
authors.lastname: Sui
authors.forename: Shuzhi
authors.initials: S
authors.lastname: He
authors.forename: Qian
authors.initials: Q
authors.lastname: Liu
authors.forename: Xing
authors.initials: X
authors.lastname: Lei
authors.forename: Zhonghong
authors.initials: Z
authors.lastname: Wu
authors.forename: Jianhui
authors.initials: J
authors.lastname: Tian
mesh_terms: D000818:Animals; D046149:Embryo Culture Techniques; D004622:Embryo, Mammalian / Q000378:metabolism*; D047108:Embryonic Development; D005260:Female; D005307:Fertilization in Vitro; D018507:Gene Expression Regulation, Developmental; D063990:Gene Ontology; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D010641:Phenotype; D011247:Pregnancy; D060066:Protein Interaction Maps; D020543:Proteome / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D040901:Proteomics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry
chemical_list: D020543:Proteome; D012333:RNA, Messenger