title: Electrospun collagen-poly(L-lactic acid-co-ε-caprolactone) membranes for cartilage tissue engineering.
abstract: OBJECTIVE
  To study the feasibility of electrospun collagen-poly(L-lactic acid-co-ε-caprolactone) (collagen-PLCL) membranes for cartilage tissue engineering.
  
  
  METHODS
  Characteristics and mechanical properties of collagen-PLCL membranes were analyzed. The cell affinity of collagen-PLCL membranes with chondrocytes was also assessed. Then, the cell-scaffold constructs were engineered with collagen-PLCL membranes seeded chondrocytes by a sandwich model. After culture for 1 week in vitro, the constructs were implanted subcutaneously into nude mice for 4, 8 and 12 weeks, followed by evaluation of the quality of neocartilage.
  
  
  RESULTS
  Collagen-PLCL membranes exhibited excellent balanced properties without cytotoxicity. With the extension of implantation time in vivo, the constructs revealed more cartilage-like tissue especially at 8 and 12 weeks. The Young's modulus of the constructs also significantly increased and neared that of native cartilage at 12 weeks postimplantation.
  
  
  CONCLUSIONS
  We suggest that collagen-PLCL membranes facilitate the formation of cartilage and thus may represent a promising scaffold for cartilage tissue engineering.
authors.affiliation: Department of Cardiothoracic Surgery, Shanghai Children's Medical Center, Shanghai Jiao Tong University School of Medicine, 1678 Dong Fang Road, Shanghai 200127, China.
authors.forename: Xiaomin
authors.initials: X
authors.lastname: He
authors.forename: Wei
authors.initials: W
authors.lastname: Fu
authors.forename: Bei
authors.initials: B
authors.lastname: Feng
authors.forename: Hao
authors.initials: H
authors.lastname: Wang
authors.forename: Zhenling
authors.initials: Z
authors.lastname: Liu
authors.forename: Meng
authors.initials: M
authors.lastname: Yin
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Jinghao
authors.initials: J
authors.lastname: Zheng
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000008:administration & dosage; D002356:Cartilage / Q000166:cytology* / Q000502:physiology; D002448:Cell Adhesion; D002454:Cell Differentiation; D049109:Cell Proliferation; D019902:Chondrocytes / Q000166:cytology*; D003094:Collagen / Q000737:chemistry* / Q000378:metabolism; D004563:Electrochemistry; D007124:Immunoenzyme Techniques; D008422:Materials Testing; D008567:Membranes, Artificial; D051379:Mice; D057139:Nanofibers; D011091:Polyesters / Q000737:chemistry* / Q000378:metabolism; D011817:Rabbits; D023822:Tissue Engineering
chemical_list: D001672:Biocompatible Materials; D008567:Membranes, Artificial; D011091:Polyesters; C550605:poly(lactic acid-co-epsilon-caprolactone); D003094:Collagen