title: Hypolipidemic effects of starch and γ-oryzanol from wx/ae double-mutant rice on BALB/c.KOR-Apoe(shl) mice.
abstract: waxy/amylose-extender (wx/ae) double-mutant japonica rice (Oryza sativa L.) produces resistant starch (RS) and a large amount of γ-oryzanol. Our previous study has shown the hypolipidemic effect of wx/ae brown rice on mice. To identify the functional constituents of the hypolipidemic activity in wx/ae rice, we prepared pure wx/ae starch and γ-oryzanol from wx/ae rice and investigated their effect on the lipid metabolism in BALB/c.KOR/Stm Slc-Apoe(shl) mice. The mice were fed for 3 weeks a diet containing non-mutant rice starch, non-mutant rice starch plus γ-oryzanol, wx/ae starch, or wx/ae starch plus γ-oryzanol. γ-Oryzanol by itself had no effect on the lipid metabolism, and wx/ae starch prevented an accumulation of triacylglycerol (TAG) in the liver. Interestingly, the combination of wx/ae starch plus γ-oryzanol not only prevented a TAG accumulation in the liver, but also partially suppressed the rise in plasma TAG concentration, indicating that wx/ae starch and γ-oryzanol could have a synergistic effect on the lipid metabolism.
authors.affiliation: Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Sakai, Osaka 599-8531, Japan.
authors.forename: Makoto
authors.initials: M
authors.lastname: Nakaya
authors.forename: Aiko
authors.initials: A
authors.lastname: Shojo
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Hirai
authors.forename: Kenji
authors.initials: K
authors.lastname: Matsumoto
authors.forename: Shinichi
authors.initials: S
authors.lastname: Kitamura
mesh_terms: D000818:Animals; D001057:Apolipoproteins E / Q000172:deficiency* / Q000235:genetics*; D001835:Body Weight / Q000187:drug effects; D002432:Cecum / Q000187:drug effects / Q000378:metabolism; D059305:Diet, High-Fat / Q000009:adverse effects; D004040:Dietary Carbohydrates / Q000009:adverse effects; D004347:Drug Interactions; D004435:Eating / Q000187:drug effects; D005234:Fatty Liver / Q000517:prevention & control; D005243:Feces; D030841:Food, Genetically Modified; D005786:Gene Expression Regulation / Q000187:drug effects; D000960:Hypolipidemic Agents / Q000494:pharmacology*; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009154:Mutation; D065626:Non-alcoholic Fatty Liver Disease; D012275:Oryza / Q000737:chemistry / Q000235:genetics*; D010666:Phenylpropionates / Q000494:pharmacology*; D013213:Starch / Q000494:pharmacology*; D013395:Sucrose / Q000009:adverse effects
chemical_list: D001057:Apolipoproteins E; D004040:Dietary Carbohydrates; D000960:Hypolipidemic Agents; D010666:Phenylpropionates; D013395:Sucrose; D013213:Starch; C013172:gamma-oryzanol