title: Amplification of progenitors in the mammalian telencephalon includes a new radial glial cell type.
abstract: The mechanisms governing the expansion of neuron number in specific brain regions are still poorly understood. Enlarged neuron numbers in different species are often anticipated by increased numbers of progenitors dividing in the subventricular zone. Here we present live imaging analysis of radial glial cells and their progeny in the ventral telencephalon, the region with the largest subventricular zone in the murine brain during neurogenesis. We observe lineage amplification by a new type of progenitor, including bipolar radial glial cells dividing at subapical positions and generating further proliferating progeny. The frequency of this new type of progenitor is increased not only in larger clones of the mouse lateral ganglionic eminence but also in cerebral cortices of gyrated species, and upon inducing gyrification in the murine cerebral cortex. This implies key roles of this new type of radial glia in ontogeny and phylogeny.
authors.affiliation: Institute of Stem Cell Research, Helmholtz Center Munich, Ingolstädter Landstr. 1, Neuherberg, 85764 Munich, Germany.
authors.forename: Gregor-Alexander
authors.initials: GA
authors.lastname: Pilz
authors.forename: Atsunori
authors.initials: A
authors.lastname: Shitamukai
authors.forename: Isabel
authors.initials: I
authors.lastname: Reillo
authors.forename: Emilie
authors.initials: E
authors.lastname: Pacary
authors.forename: Julia
authors.initials: J
authors.lastname: Schwausch
authors.forename: Ronny
authors.initials: R
authors.lastname: Stahl
authors.forename: Jovica
authors.initials: J
authors.lastname: Ninkovic
authors.forename: Hugo J
authors.initials: HJ
authors.lastname: Snippert
authors.forename: Hans
authors.initials: H
authors.lastname: Clevers
authors.forename: Leanne
authors.initials: L
authors.lastname: Godinho
authors.forename: Francois
authors.initials: F
authors.lastname: Guillemot
authors.forename: Victor
authors.initials: V
authors.lastname: Borrell
authors.forename: Fumio
authors.initials: F
authors.lastname: Matsuzaki
authors.forename: Magdalena
authors.initials: M
authors.lastname: Götz
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D019070:Cell Lineage / Q000502:physiology; D049109:Cell Proliferation; D004622:Embryo, Mammalian; D063928:Ependymoglial Cells / Q000166:cytology* / Q000378:metabolism; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins; D051379:Mice; D008822:Mice, Transgenic; D058953:Neural Stem Cells / Q000166:cytology* / Q000378:metabolism; D055495:Neurogenesis; D009474:Neurons / Q000166:cytology* / Q000378:metabolism; D013687:Telencephalon / Q000166:cytology* / Q000196:embryology / Q000378:metabolism; D059008:Time-Lapse Imaging; D046509:Tissue Culture Techniques
chemical_list: D049452:Green Fluorescent Proteins