title: [The retrospective study of serum aspergillus galactomannan (GM) antigen assay in invasive aspergillosis on hematological diseases].
abstract: OBJECTIVE
  To explore the relationship between the optical density index of serum aspergillus galactomannan (GM) assay and invasive aspergillosis (IA).
  
  
  METHODS
  From Jan 2008 to Dec 2011, 825 hematological diseases patients with neutrophil count <0.5×10⁹/L⁹ by continuous blood count tests were admitted into our hospital. The optical density index of GM assay was ≥0.5 at least once. Of 825 patients, 247 cases were manifested as fever during hospitalization. The optical density index of GM antigen was detected by enzyme-linked immunosorbent assay, and the sensitivity and specificity of optical density ranged in 0.5-1.5.
  
  
  RESULTS
  In this study, the sensitivity and specificity of GM assay with continuous twice samples (73% and 93%, respectively) were higher than single sample (66% and 80%, respectively) when optical density index ≥1.0. 69 cases were diagnosed as proven IA with the incidence rate of 8.36%.
  
  
  CONCLUSIONS
  The cut-off level for serum GM antigen assay should be decided as optical density index in two continuous samples of ≥1.0.
authors.affiliation: Ruijin Hospital, Medical College of Shanghai Jiaotong University, Shanghai 200025, China.
authors.forename: Yan-yan
authors.initials: YY
authors.lastname: Wang
authors.forename: Chen-lu
authors.initials: CL
authors.lastname: Xiao
authors.forename: Jun-min
authors.initials: JM
authors.lastname: Li
authors.forename: Wei-li
authors.initials: WL
authors.lastname: Zhao
authors.forename: Jian-qing
authors.initials: JQ
authors.lastname: Mi
authors.forename: Jiong
authors.initials: J
authors.lastname: Hu
authors.forename: Yu-xing
authors.initials: YX
authors.lastname: Ni
authors.forename: Zhi-xiang
authors.initials: ZX
authors.lastname: Shen
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000946:Antigens, Fungal / Q000097:blood*; D001228:Aspergillosis / Q000097:blood / Q000175:diagnosis* / Q000209:etiology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005690:Galactose / Q000031:analogs & derivatives; D006402:Hematologic Diseases / Q000097:blood* / Q000382:microbiology; D006801:Humans; D008297:Male; D008351:Mannans / Q000097:blood / Q000276:immunology; D008875:Middle Aged; D012680:Sensitivity and Specificity; D055815:Young Adult
chemical_list: D000946:Antigens, Fungal; D008351:Mannans; C012990:galactomannan; D005690:Galactose