title: Expression profiling of Aldh1l1-precursors in the developing spinal cord reveals glial lineage-specific genes and direct Sox9-Nfe2l1 interactions.
abstract: Developmental regulation of gliogenesis in the mammalian CNS is incompletely understood, in part due to a limited repertoire of lineage-specific genes. We used Aldh1l1-GFP as a marker for gliogenic radial glia and later-stage precursors of developing astrocytes and performed gene expression profiling of these cells. We then used this dataset to identify candidate transcription factors that may serve as glial markers or regulators of glial fate. Our analysis generated a database of developmental stage-related markers of Aldh1l1+ cells between murine embryonic day 13.5-18.5. Using these data we identify the bZIP transcription factor Nfe2l1 and demonstrate that it promotes glial fate under direct Sox9 regulatory control. Thus, this dataset represents a resource for identifying novel regulators of glial development.
authors.affiliation: Department of Pediatrics, Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, University of California San Francisco, 513 Parnassus Avenue, San Francisco, California 94143, USA.
authors.forename: Anna V
authors.initials: AV
authors.lastname: Molofsky
authors.forename: Stacey M
authors.initials: SM
authors.lastname: Glasgow
authors.forename: Lesley S
authors.initials: LS
authors.lastname: Chaboub
authors.forename: Hui-Hsin
authors.initials: HH
authors.lastname: Tsai
authors.forename: Alice T
authors.initials: AT
authors.lastname: Murnen
authors.forename: Kevin W
authors.initials: KW
authors.lastname: Kelley
authors.forename: Stephen P J
authors.initials: SP
authors.lastname: Fancy
authors.forename: Tracy J
authors.initials: TJ
authors.lastname: Yuen
authors.forename: Lohith
authors.initials: L
authors.lastname: Madireddy
authors.forename: Sergio
authors.initials: S
authors.lastname: Baranzini
authors.forename: Benjamin
authors.initials: B
authors.lastname: Deneen
authors.forename: David H
authors.initials: DH
authors.lastname: Rowitch
authors.forename: Michael C
authors.initials: MC
authors.lastname: Oldham
mesh_terms: D000367:Age Factors; D000080924:Aldehyde Dehydrogenase 1 Family; D000818:Animals; D002454:Cell Differentiation; D002478:Cells, Cultured; D002645:Chickens; D019295:Computational Biology; D018274:Electroporation; D004622:Embryo, Mammalian; D005434:Flow Cytometry; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D005904:Glial Fibrillary Acidic Protein; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008822:Mice, Transgenic; D051265:NF-E2-Related Factor 1 / Q000235:genetics / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism; D009457:Neuroglia / Q000378:metabolism*; D009474:Neurons / Q000145:classification / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D050697:Retinal Dehydrogenase / Q000235:genetics / Q000378:metabolism*; D055758:SOX9 Transcription Factor / Q000235:genetics / Q000378:metabolism*; D013116:Spinal Cord / Q000166:cytology* / Q000196:embryology; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
chemical_list: D005904:Glial Fibrillary Acidic Protein; D007527:Isoenzymes; D051265:NF-E2-Related Factor 1; D009419:Nerve Tissue Proteins; C093714:Nfe2L1 protein, mouse; D055758:SOX9 Transcription Factor; C527933:Sox9 protein, mouse; D014157:Transcription Factors; D049452:Green Fluorescent Proteins; D000080924:Aldehyde Dehydrogenase 1 Family; C515028:ALDH1A1 protein, mouse; D050697:Retinal Dehydrogenase
keywords: Nfe2l1; astrocytes; expression profiling; gene coexpression; gliogenesis