title: No association between IRS‑1 promoter methylation and type 2 diabetes.
abstract: As a candidate gene for type 2 diabetes (T2D), insulin receptor substrate-1 (IRS‑1) gene variations were found to be associated with the risk of T2D. The aim of our study was to investigate the contribution of promoter DNA methylation of the IRS‑1 gene to the risk of T2D. Using bisulphite pyrosequencing technology, the DNA methylation levels of 3 CpG dinucleotides within the IRS‑1 gene promoter were measured in 48 T2D patients and 48 age‑ and gender‑matched healthy controls. Our results indicated that there was no significant association between the methylation of the IRS‑1 gene promoter and the risk of T2D (P>0.1). A breakdown analysis by gender revealed that IRS‑1 promoter methylation was not associated with an increased risk of T2D for either gender (P>0.1), although there were significantly lower methy-lation levels of CpG1 (P=0.002) and CpG2 (P=0.043) within the IRS‑1 gene promoter in males than in females.
authors.affiliation: Zhejiang Provincial People's Hospital, Hangzhou, Zhejiang 310014, P.R. China.
authors.forename: Jiangbo
authors.initials: J
authors.lastname: Ma
authors.forename: Jia
authors.initials: J
authors.lastname: Cheng
authors.forename: Lingyan
authors.initials: L
authors.lastname: Wang
authors.forename: Hongwei
authors.initials: H
authors.lastname: Wang
authors.forename: Leiting
authors.initials: L
authors.lastname: Xu
authors.forename: Panpan
authors.initials: P
authors.lastname: Liu
authors.forename: Shizhong
authors.initials: S
authors.lastname: Bu
authors.forename: Lina
authors.initials: L
authors.lastname: Zhang
authors.forename: Yanping
authors.initials: Y
authors.lastname: Le
authors.forename: Meng
authors.initials: M
authors.lastname: Ye
authors.forename: Qinwen
authors.initials: Q
authors.lastname: Wang
authors.forename: Yuping
authors.initials: Y
authors.lastname: Shi
authors.forename: Shiwei
authors.initials: S
authors.lastname: Duan
mesh_terms: D000328:Adult; D000368:Aged; D018899:CpG Islands; D019175:DNA Methylation; D003924:Diabetes Mellitus, Type 2 / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D005260:Female; D006801:Humans; D055504:Insulin Receptor Substrate Proteins / Q000235:genetics* / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D010641:Phenotype; D011401:Promoter Regions, Genetic; D012307:Risk Factors; D017422:Sequence Analysis, DNA; D012737:Sex Factors
chemical_list: D055504:Insulin Receptor Substrate Proteins