title: Hemocompatibility of polymeric nanostructured surfaces.
abstract: Tissue integration is an important property when inducing transplant tolerance, however, the hemocompatibility of the biomaterial surface also plays an important role in the ultimate success of the implant. Therefore, in order to induce transplant tolerance, it is critical to understand the interaction of blood components with the material surfaces. In this study, we have investigated the adsorption of key blood serum proteins, in vitro adhesion and activation of platelets and clotting kinetics of whole blood on flat polycaprolactone (PCL) surfaces, nanowire (NW) surfaces and nanofiber (NF) surfaces. Previous studies have shown that polymeric nanostructured surfaces improve cell adhesion, proliferation and viability; however it is unclear how these polymeric nanostructured surfaces interact with the blood and its components. Protein adsorption results indicate that while there were no significant differences in total albumin (ALB) adsorption on PCL, NW and NF surfaces, NW surfaces had higher total fibrinogen (FIB) and immunoglobulin-G (IgG) adsorption compared to NF and PCL surfaces. In contrast, NF surfaces had higher surface FIB and IgG adsorption compared to PCL and NW surfaces. Platelet adhesion and viability studies show more adhesion and clustering of platelets on the NF surfaces as compared to PCL and NW surfaces. Platelet activation studies reveal that NW surfaces have the highest percentage of unactivated platelets, whereas NF surfaces have the highest percentage of fully activated platelets. Whole blood clotting results indicate that NW surfaces maintain an increased amount of free hemoglobin during the clotting process compared to PCL and NF surface, indicating less clotting and slower rate of clotting on their surfaces.
authors.affiliation: Department of Mechanical Engineering, Colorado State University, Fort Collins, CO, 80523, USA.
authors.forename: Victoria
authors.initials: V
authors.lastname: Leszczak
authors.forename: Barbara S
authors.initials: BS
authors.lastname: Smith
authors.forename: Ketul C
authors.initials: KC
authors.lastname: Popat
mesh_terms: D000327:Adsorption; D001672:Biocompatible Materials / Q000737:chemistry*; D001777:Blood Coagulation; D001798:Blood Proteins / Q000737:chemistry; D005340:Fibrinogen / Q000737:chemistry; D006801:Humans; D007074:Immunoglobulin G / Q000737:chemistry; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D057139:Nanofibers / Q000737:chemistry / Q000648:ultrastructure; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D053770:Nanowires / Q000737:chemistry / Q000648:ultrastructure; D015539:Platelet Activation; D011091:Polyesters / Q000737:chemistry*; D012709:Serum Albumin / Q000737:chemistry; D013499:Surface Properties
chemical_list: D001672:Biocompatible Materials; D001798:Blood Proteins; D007074:Immunoglobulin G; D011091:Polyesters; D012709:Serum Albumin; C016240:polycaprolactone; D005340:Fibrinogen