title: Expression of FAS ligand in the ipsilateral and contralateral testicles of rats subjected to the torsion of the unilateral testicular cord.
abstract: OBJECTIVE
  To investigate the expression of FAS ligand (FASL) in ipsilateral and contralateral testicles of rats submitted to ischemia/reperfusion.
  
  
  METHODS
  Wistar rats (n=21) distributed into groups control (GC), n=5, testicular exposure; ischemia (GI), (n=8), Torsion in the left testicular Cord (TCT) for three hours followed by orchiectomy without distortion and orchietomy of the contralateral testicle after 24 hours; and reperfusion (GR), (n=8), left TCT for 3 hours and distortion and repositioning on the scrotum and bilateral orchiectomy after 24 hours. Quantification of the FASL expression by immune-histochemistry.
  
  
  RESULTS
  Statistical analysis showed similarity between GC and GI (p>0.05), differences detected are concentrated on the GR (p<0.05), increase in immunoexpression of FASL in the subgroups Right GR (406.8+-61.5) and Left GR (135.3 +-28.9) with significant predominance in the GR subgroup.
  
  
  CONCLUSIONS
  Ischemia/reperfusion increased the FASL expression significantly in contralateral testicles in GR, in rats.
authors.affiliation: Health and Development, Postgraduate Program, Mato Grosso do Sul Federal University (UFMS), Campo Grande-MS, Brazil.
authors.forename: Peterson Vieira de
authors.initials: PV
authors.lastname: Assis
authors.forename: Ricardo Dutra
authors.initials: RD
authors.lastname: Aydos
authors.forename: Iandara Schettert
authors.initials: IS
authors.lastname: Silva
authors.forename: Guido
authors.initials: G
authors.lastname: Marks
authors.forename: Luis Carlos
authors.initials: LC
authors.lastname: Takita
authors.forename: Marco Antonio
authors.initials: MA
authors.lastname: Gonçalves
authors.forename: Rondon Tosta
authors.initials: RT
authors.lastname: Ramalho
mesh_terms: D000818:Animals; D053222:Fas Ligand Protein / Q000032:analysis*; D007150:Immunohistochemistry; D019194:Ischemic Preconditioning / Q000379:methods*; D008297:Male; D009919:Orchiectomy; D051381:Rats; D017208:Rats, Wistar; D013086:Spermatic Cord Torsion / Q000378:metabolism*; D013737:Testis / Q000098:blood supply / Q000378:metabolism*; D013997:Time Factors
chemical_list: D053222:Fas Ligand Protein