title: Optimizing fluorescent protein choice for transgenic embryonic medaka models.
abstract: Early-life-stage transgenic medaka are recognized as a pertinent model by the Organisation for Economic Co-operation and Development and are noncompliant with the European definition of a laboratory animal. However, autofluorescence confounds readout of fluorescent biomarkers. The authors determined the fluorescence emission spectrum of different embryonic stages of medaka submitted to a range of excitation wavelengths. This allows selection of high signal-to-noise ratio fluorescent proteins and combining multiple biomarkers within a single embryo.
authors.affiliation: WatchFrog, Evry, France.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Loire
authors.forename: Emilie
authors.initials: E
authors.lastname: Barbeau
authors.forename: Gregory F
authors.initials: GF
authors.lastname: Lemkine
authors.forename: Marc A
authors.initials: MA
authors.lastname: Léonard
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Tindall
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D015415:Biomarkers / Q000378:metabolism; D004527:Egg Proteins / Q000235:genetics; D005453:Fluorescence; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism*; D008751:Methylene Blue; D009990:Oryzias / Q000196:embryology* / Q000235:genetics / Q000378:metabolism*; D011498:Protein Precursors / Q000235:genetics; D059629:Signal-To-Noise Ratio
chemical_list: D015415:Biomarkers; D004527:Egg Proteins; D011498:Protein Precursors; C104950:choriogenin H; D049452:Green Fluorescent Proteins; D008751:Methylene Blue
keywords: Autofluorescence; Embryo; Fluorescent protein; Medaka