title: Multicomponent chemical imaging of pharmaceutical solid dosage forms with broadband CARS microscopy.
abstract: We compare a coherent Raman imaging modality, broadband coherent anti-Stokes Raman scattering (BCARS) microscopy, with spontaneous Raman microscopy for quantitative and qualitative assessment of multicomponent pharmaceuticals. Indomethacin was used as a model active pharmaceutical ingredient (API) and was analyzed in a tabulated solid dosage form, embedded within commonly used excipients. In comparison with wide-field spontaneous Raman chemical imaging, BCARS acquired images 10× faster, at higher spatiochemical resolution and with spectra of much higher SNR, eliminating the need for multivariate methods to identify chemical components. The significant increase in spatiochemical resolution allowed identification of an unanticipated API phase that was missed by the spontaneous wide-field method and bulk Raman spectroscopy. We confirmed the presence of the unanticipated API phase using confocal spontaneous Raman, which provided spatiochemical resolution similar to BCARS but at 100× slower acquisition times.
authors.affiliation: Materials Measurement Laboratory, National Institute of Standards and Technology, Gaithersburg, Maryland 20899, USA.
authors.forename: Christopher M
authors.initials: CM
authors.lastname: Hartshorn
authors.forename: Young Jong
authors.initials: YJ
authors.lastname: Lee
authors.forename: Charles H
authors.initials: CH
authors.lastname: Camp
authors.forename: Zhen
authors.initials: Z
authors.lastname: Liu
authors.forename: John
authors.initials: J
authors.lastname: Heddleston
authors.forename: Nicole
authors.initials: N
authors.lastname: Canfield
authors.forename: Timothy A
authors.initials: TA
authors.lastname: Rhodes
authors.forename: Angela R
authors.initials: AR
authors.lastname: Hight Walker
authors.forename: Patrick J
authors.initials: PJ
authors.lastname: Marsac
authors.forename: Marcus T
authors.initials: MT
authors.lastname: Cicerone
mesh_terms: D004304:Dosage Forms; D008853:Microscopy / Q000379:methods*; D004364:Pharmaceutical Preparations / Q000032:analysis* / Q000737:chemistry; D013059:Spectrum Analysis, Raman / Q000379:methods*; D014961:X-Ray Diffraction / Q000379:methods
chemical_list: D004304:Dosage Forms; D004364:Pharmaceutical Preparations