title: The transcription analysis of duck enteritis virus UL49.5 gene using real-time quantitative reverse transcription PCR.
abstract: Duck enteritis virus (DEV) UL49.5 encoding glycoprotein N was a conserved gene. The transcription dynamic process of UL49.5 homologous genes in herpesviruses was reported. However, the transcription dynamic process of DEV UL49.5 gene has not yet been established. In this study, a real-time quantitative reverse transcription PCR (real-time qRT-PCR) assay was established to test the transcription dynamic process of DEV UL49.5 gene, and the recombinant plasmid pUCm-T/UL49.5 was constructed as the standard DNA. The samples prepared from DEV-infected (at different time points) and uninfected cell were detected and calculated. The results demonstrated that the real-time qRT-PCR assay was successfully established. The transcription product of DEV UL49.5 gene was first detected at 0.5 h post infection (p.i.), increased at 8 h p.i. and reached a peak at 60 h p.i. Our results illustrated that DEV UL49.5 gene could be regarded as a late gene. The transcription dynamic process of DEV UL49.5 gene may provide a significant clue for further studies of DEV UL49.5 gene.
authors.affiliation: Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, 611130, China, linmeng0202@126.com.
authors.forename: Meng
authors.initials: M
authors.lastname: Lin
authors.forename: Renyong
authors.initials: R
authors.lastname: Jia
authors.forename: Mingshu
authors.initials: M
authors.lastname: Wang
authors.forename: Xinghong
authors.initials: X
authors.lastname: Gao
authors.forename: Dekang
authors.initials: D
authors.lastname: Zhu
authors.forename: Shun
authors.initials: S
authors.lastname: Chen
authors.forename: Zhongqiong
authors.initials: Z
authors.lastname: Yin
authors.forename: Yin
authors.initials: Y
authors.lastname: Wang
authors.forename: Xiaoyue
authors.initials: X
authors.lastname: Chen
authors.forename: Anchun
authors.initials: A
authors.lastname: Cheng
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D004372:Ducks; D005347:Fibroblasts / Q000821:virology; D020869:Gene Expression Profiling; D006023:Glycoproteins / Q000096:biosynthesis* / Q000235:genetics; D025922:Mardivirus / Q000235:genetics* / Q000254:growth & development; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors; D014158:Transcription, Genetic; D015678:Viral Structural Proteins / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D006023:Glycoproteins; D015678:Viral Structural Proteins