title: Rapid and sensitive detection of β-agonists using a portable fluorescence biosensor based on fluorescent nanosilica and a lateral flow test strip.
abstract: A portable fluorescence biosensor with rapid and ultrasensitive response for Clenbuterol (CL) has been built up with fluorescent nanosilica and a lateral flow test strip. Quantitative detection of CL was realized by recording the fluorescence intensity of fluorescent nanosilica captured on the test line. The sensing results indicated that the sensitivity of the fluorescent nanosilica-based strip was better than that of conventional colloidal gold-based strips. The visual limit of detection of the strip for qualitative detection was 0.1 ng/mL while the LOD for quantitative detection could down to 0.037 ng/mL by using fluorescence biosensor. The recoveries of test samples were from 89.3% to 97.7%. The assay time for CL detection was less than 8 min, suitable for rapid testing on-site.
authors.affiliation: School of Food Science and Technology, Jiangnan University, Lihu road 1800, Wuxi, Jiangsu Province 214122, China; Key Laboratory of Animal Immunology of the Ministry of Agriculture, Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Huayuan Road 116, Zhengzhou, Henan Province 450002, China.
authors.forename: Chunmei
authors.initials: C
authors.lastname: Song
authors.forename: Aimin
authors.initials: A
authors.lastname: Zhi
authors.forename: Qingtang
authors.initials: Q
authors.lastname: Liu
authors.forename: Jifei
authors.initials: J
authors.lastname: Yang
authors.forename: Guochao
authors.initials: G
authors.lastname: Jia
authors.forename: Jahanian
authors.initials: J
authors.lastname: Shervin
authors.forename: Liang
authors.initials: L
authors.lastname: Tang
authors.forename: Xiaofei
authors.initials: X
authors.lastname: Hu
authors.forename: Ruiguang
authors.initials: R
authors.lastname: Deng
authors.forename: Chuanlai
authors.initials: C
authors.lastname: Xu
authors.forename: Gaiping
authors.initials: G
authors.lastname: Zhang
mesh_terms: D000318:Adrenergic beta-Agonists / Q000652:urine*; D000818:Animals; D015374:Biosensing Techniques / Q000295:instrumentation*; D002976:Clenbuterol / Q000652:urine*; D004867:Equipment Design; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry*; D057230:Limit of Detection; D011934:Reagent Strips; D012822:Silicon Dioxide / Q000737:chemistry*; D013552:Swine
chemical_list: D000318:Adrenergic beta-Agonists; D005456:Fluorescent Dyes; D011934:Reagent Strips; D012822:Silicon Dioxide; D002976:Clenbuterol
keywords: Clenbuterol; Fluorescence biosensor; Fluorescent nanosilica; Strip