title: Incorporation of L-lysine and 2,2'-dipyridyl in the growth media promotes desferrioxamine E production by an actinobacterium.
abstract: The study describes the use of the chelating agent 2,2'-dipyridyl in conjunction with lysine to increase the production of the siderophore desferrioxamine E by a previously described actinobacterium 23F. Desferrioxamine E is a type of siderophore known to be produced by Streptomycete species. Lysine is a precursor of the siderophore and its presence in the culture medium is known to promote desferrioxamine E synthesis. The further addition of 2,2'-dipyridyl was found to enhance production of the siderophore in the presence of lysine (5 g l(-1)) nearly twofold when incorporated at a concentration of 200 μM. Increasing the concentration of the chelating agent above 200 μM resulted in a decrease in siderophore production. The role of the chelating agent was thought to be in creating iron-limiting conditions in the culture medium and so promoting the induction of the desferrioxamine E biosynthetic pathway. This medium is likely to be a useful tool in the screening for producers of desferrioxamine E.
authors.affiliation: School of Pharmacy and Biomolecular Sciences, Liverpool John Moores University, Byrom Street, Liverpool, L3 3AF, UK.
authors.forename: Ismini
authors.initials: I
authors.lastname: Nakouti
authors.forename: Glyn
authors.initials: G
authors.lastname: Hobbs
mesh_terms: D015082:2,2'-Dipyridyl / Q000378:metabolism*; D039903:Actinobacteria / Q000254:growth & development / Q000378:metabolism*; D002614:Chelating Agents / Q000378:metabolism; D003470:Culture Media / Q000737:chemistry*; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects; D006877:Hydroxamic Acids / Q000378:metabolism*; D007769:Lactams / Q000378:metabolism*; D008239:Lysine / Q000378:metabolism*; D015533:Transcriptional Activation
chemical_list: D002614:Chelating Agents; D003470:Culture Media; D006877:Hydroxamic Acids; D007769:Lactams; C561576:desferrioxamine E; D015082:2,2'-Dipyridyl; D008239:Lysine