title: Mild traumatic brain injury results in depressed cerebral glucose uptake: An (18)FDG PET study.
abstract: Moderate to severe traumatic brain injury (TBI) in humans and rats induces measurable metabolic changes, including a sustained depression in cerebral glucose uptake. However, the effect of a mild TBI on brain glucose uptake is unclear, particularly in rodent models. This study aimed to determine the glucose uptake pattern in the brain after a mild lateral fluid percussion (LFP) TBI. Briefly, adult male rats were subjected to a mild LFP and positron emission tomography (PET) imaging with (18)F-fluorodeoxyglucose ((18)FDG), which was performed prior to injury and at 3 and 24 h and 5, 9, and 16 days post-injury. Locomotor function was assessed prior to injury and at 1, 3, 7, 14, and 21 days after injury using modified beam walk tasks to confirm injury severity. Histology was performed at either 10 or 21 days post-injury. Analysis of function revealed a transient impairment in locomotor ability, which corresponds to a mild TBI. Using reference region normalization, PET imaging revealed that mild LFP-induced TBI depresses glucose uptake in both the ipsilateral and contralateral hemispheres in comparison with sham-injured and naïve controls from 3 h to 5 days post-injury. Further, areas of depressed glucose uptake were associated with regions of glial activation and axonal damage, but no measurable change in neuronal loss or gross tissue damage was observed. In conclusion, we show that mild TBI, which is characterized by transient impairments in function, axonal damage, and glial activation, results in an observable depression in overall brain glucose uptake using (18)FDG-PET.
authors.affiliation: 1 Department of Radiology, Uniformed Services University of the Health Sciences , Bethesda, Maryland.
authors.forename: Reed
authors.initials: R
authors.lastname: Selwyn
authors.forename: Nicole
authors.initials: N
authors.lastname: Hockenbury
authors.forename: Shalini
authors.initials: S
authors.lastname: Jaiswal
authors.forename: Sanjeev
authors.initials: S
authors.lastname: Mathur
authors.forename: Regina C
authors.initials: RC
authors.lastname: Armstrong
authors.forename: Kimberly R
authors.initials: KR
authors.lastname: Byrnes
mesh_terms: D000818:Animals; D001921:Brain / Q000000981:diagnostic imaging* / Q000378:metabolism* / Q000473:pathology; D001930:Brain Injuries / Q000000981:diagnostic imaging* / Q000378:metabolism* / Q000473:pathology; D020833:Diffuse Axonal Injury / Q000000981:diagnostic imaging / Q000378:metabolism; D019788:Fluorodeoxyglucose F18; D007839:Functional Laterality; D005947:Glucose / Q000378:metabolism*; D008297:Male; D049268:Positron-Emission Tomography; D011597:Psychomotor Performance; D019275:Radiopharmaceuticals; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D019275:Radiopharmaceuticals; D019788:Fluorodeoxyglucose F18; D005947:Glucose