title: A new murine model of endovascular aortic aneurysm repair.
abstract: Endovascular aneurysm exclusion is a validated technique to prevent aneurysm rupture. Long-term results highlight technique limitations and new aspects of Abdominal aortic aneurysm (AAA) pathophysiology. There is no abdominal aortic aneurysm endograft exclusion model cheap and reproducible, which would allow deep investigations of AAA before and after treatment. We hereby describe how to induce, and then to exclude with a covered coronary stentgraft an abdominal aortic aneurysm in a rat. The well known elastase induced AAA model was first reported in 1990(1) in a rat, then described in mice(2). Elastin degradation leads to dilation of the aorta with inflammatory infiltration of the abdominal wall and intra luminal thrombus, matching with human AAA. Endovascular exclusion with small covered stentgraft is then performed, excluding any interactions between circulating blood and the aneurysm thrombus. Appropriate exclusion and stentgraft patency is confirmed before euthanasia by an angiography thought the left carotid artery. Partial control of elastase diffusion makes aneurysm shape different for each animal. It is difficult to create an aneurysm, which will allow an appropriate length of aorta below the aneurysm for an easy stentgraft introduction, and with adequate proximal and distal neck to prevent endoleaks. Lots of failure can result to stentgraft introduction which sometimes lead to aorta tear with pain and troubles to stitch it, and endothelial damage with post op aorta thrombosis. Giving aspirin to rats before stentgraft implantation decreases failure rate without major hemorrhage. Clamping time activates neutrophils, endothelium and platelets, and may interfere with biological analysis.
authors.affiliation: INSERM U698 Cardiovascular Remodelling, Hôpital X. Bichat, AP-HP, Paris. mrouer@gmail.com
authors.forename: Martin
authors.initials: M
authors.lastname: Rouer
authors.forename: Olivier
authors.initials: O
authors.lastname: Meilhac
authors.forename: Sandrine
authors.initials: S
authors.lastname: Delbosc
authors.forename: Liliane
authors.initials: L
authors.lastname: Louedec
authors.forename: Graciela
authors.initials: G
authors.lastname: Pavon-Djavid
authors.forename: Jane
authors.initials: J
authors.lastname: Cross
authors.forename: Josette
authors.initials: J
authors.lastname: Legagneux
authors.forename: Maxime
authors.initials: M
authors.lastname: Bouilliant-Linet
authors.forename: Jean-Baptiste
authors.initials: JB
authors.lastname: Michel
authors.forename: Jean-Marc
authors.initials: JM
authors.lastname: Alsac
mesh_terms: D000818:Animals; D017544:Aortic Aneurysm, Abdominal / Q000601:surgery*; D004195:Disease Models, Animal; D057510:Endovascular Procedures / Q000379:methods*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D015607:Stents