title: Characterization of blaCTX-M IncFII plasmids and clones of Escherichia coli from pets in France.
abstract: OBJECTIVE
  To characterize bla(CTX-M) IncFII plasmids and clones of Escherichia coli from cats and dogs and to compare them with bla(CTX-M) IncFII plasmids reported in humans.
  
  
  METHODS
  From December 2006 to April 2010, 518 E. coli isolates from clinical infections in cats and dogs were screened for extended-spectrum β-lactamase (ESBL) production. Antimicrobial susceptibility was performed by disc diffusion and resistance genes were identified by PCR and sequencing. Plasmids were characterized using PCR-based replicon typing and sub-typing schemes, restriction fragment length polymorphism analysis, S1-PFGE and Southern hybridization. Isolates were characterized by PFGE, phylogenetic grouping, O25b typing and multilocus sequence typing.
  
  
  RESULTS
  Nineteen E. coli isolates (3.7%) produced ESBLs, of which 14 (74%) carried bla(CTX-M) IncFII plasmids. The bla(CTX-M) gene was predominant and located on F31:A4:B1, F36:A4:B1 or F36:A1:B20 plasmids, abundantly reported in humans. The bla(CTX-M) F22:A1:B20 or F2:A2:B20 plasmids were also found. Different sequence types (STs) were identified, such as ST10, ST410, ST359, ST617 and ST224. Only one E. coli isolate belonged to the ST131 E. coli clone and carried a bla(CTX-M) F2:A2:B20 plasmid.
  
  
  CONCLUSIONS
  This is the first known extensive study on ESBL-producing E. coli isolates from pets in France. The ST131 clone was rare. However, the predominance of human-like bla(CTX-M) IncFII plasmids suggests exchanges of these plasmids with the human reservoir.
authors.affiliation: Unité Antibiorésistance et Virulence Bactériennes, ANSES Site de Lyon, 31 avenue Tony Garnier, 69364 Lyon, France.
authors.forename: Safia
authors.initials: S
authors.lastname: Dahmen
authors.forename: Marisa
authors.initials: M
authors.lastname: Haenni
authors.forename: Pierre
authors.initials: P
authors.lastname: Châtre
authors.forename: Jean-Yves
authors.initials: JY
authors.lastname: Madec
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002371:Cat Diseases / Q000453:epidemiology* / Q000382:microbiology; D002415:Cats; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000235:genetics; D004283:Dog Diseases / Q000453:epidemiology* / Q000382:microbiology; D004285:Dogs; D004926:Escherichia coli / Q000145:classification / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification; D004927:Escherichia coli Infections / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D005602:France; D005798:Genes, Bacterial; D005838:Genotype; D008826:Microbial Sensitivity Tests; D058889:Molecular Typing; D057805:Pets; D010802:Phylogeny; D010957:Plasmids / Q000032:analysis*; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D001618:beta-Lactamases / Q000235:genetics / Q000378:metabolism*
chemical_list: D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D001618:beta-Lactamases
keywords: CTX-M; E. coli; IncF; veterinary