title: Daptomycin antibiotic production processes in fed-batch fermentation by Streptomyces roseosporus NRRL11379 with precursor effect and medium optimization.
abstract: Sodium decanoate was first found to be an effective precursor for synthesis of daptomycin from Streptomyces roseosporus NRRL11379 which was increased to 71.55-fold, compared with decanoic acid. The optimal flow rate of precursor was at 600 mg/(L day) after 48 h fermentation. From protein analysis via SDS-PAGE and identification of Tandem MS/MS afterwards, it deciphered that guanosine pentaphosphate synthetase, PNPase, tripeptidylamino peptidase primarily dealing with daptomycin synthesis. By applying Taguchi's L16 in culture optimization, the best yield was obtained from the medium with 60 g/L dextrin, 10 g/L dextrose, 1.0 g/L molasses, and 8 g/L yeast extract, respectively. The fed-batch fermentation, applied with feedback control of dextrin, stimulated the production up to 812 mg/L at 288 h. To our best knowledge, the daptomycin production in this study is significantly higher than that in previous studies and can make it more widely used in pharmaceutical industry.
authors.affiliation: Department of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, 361005, China, yswu@xmu.edu.cn.
authors.forename: I-Son
authors.initials: IS
authors.lastname: Ng
authors.forename: Chiming
authors.initials: C
authors.lastname: Ye
authors.forename: Zhixiang
authors.initials: Z
authors.lastname: Zhang
authors.forename: Yinghua
authors.initials: Y
authors.lastname: Lu
authors.forename: Keju
authors.initials: K
authors.lastname: Jing
mesh_terms: D000900:Anti-Bacterial Agents / Q000096:biosynthesis*; D019149:Bioreactors; D002851:Chromatography, High Pressure Liquid; D003470:Culture Media; D017576:Daptomycin / Q000096:biosynthesis*; D004591:Electrophoresis, Polyacrylamide Gel; D005285:Fermentation; D008855:Microscopy, Electron, Scanning; D013302:Streptomyces / Q000378:metabolism*; D053719:Tandem Mass Spectrometry
chemical_list: D000900:Anti-Bacterial Agents; D003470:Culture Media; D017576:Daptomycin