title: Comparison of generated parallel capillary arrays to three-dimensional reconstructed capillary networks in modeling oxygen transport in discrete microvascular volumes.
abstract: OBJECTIVE
  We compare RMN to PCA under several simulated physiological conditions to determine how the use of different vascular geometry affects oxygen transport solutions.
  
  
  METHODS
  Three discrete networks were reconstructed from intravital video microscopy of rat skeletal muscle (84 × 168 × 342 μm, 70 × 157 × 268 μm, and 65 × 240 × 571 μm), and hemodynamic measurements were made in individual capillaries. PCAs were created based on statistical measurements from RMNs. Blood flow and O₂ transport models were applied, and the resulting solutions for RMN and PCA models were compared under four conditions (rest, exercise, ischemia, and hypoxia).
  
  
  RESULTS
  Predicted tissue PO₂ was consistently lower in all RMN simulations compared to the paired PCA. PO₂ for 3D reconstructions at rest were 28.2 ± 4.8, 28.1 ± 3.5, and 33.0 ± 4.5 mmHg for networks I, II, and III compared to the PCA mean values of 31.2 ± 4.5, 30.6 ± 3.4, and 33.8 ± 4.6 mmHg. Simulated exercise yielded mean tissue PO₂ in the RMN of 10.1 ± 5.4, 12.6 ± 5.7, and 19.7 ± 5.7 mmHg compared to 15.3 ± 7.3, 18.8 ± 5.3, and 21.7 ± 6.0 in PCA.
  
  
  CONCLUSIONS
  These findings suggest that volume matched PCA yield different results compared to reconstructed microvascular geometries when applied to O₂ transport modeling; the predominant characteristic of this difference being an over estimate of mean tissue PO₂. Despite this limitation, PCA models remain important for theoretical studies as they produce PO₂ distributions with similar shape and parameter dependence as RMN.
authors.affiliation: Department of Medical Biophysics, University of Western Ontario, London, Ontario, Canada.
authors.forename: Graham M
authors.initials: GM
authors.lastname: Fraser
authors.forename: Daniel
authors.initials: D
authors.lastname: Goldman
authors.forename: Christopher G
authors.initials: CG
authors.lastname: Ellis
mesh_terms: D000818:Animals; D001693:Biological Transport, Active / Q000502:physiology; D002196:Capillaries / Q000502:physiology*; D008297:Male; D008833:Microcirculation / Q000502:physiology*; D008955:Models, Cardiovascular; D018482:Muscle, Skeletal / Q000098:blood supply*; D010100:Oxygen / Q000097:blood*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D010100:Oxygen
keywords: blood flow; computational model; oxygen transport modeling; parallel capillary networks; three-dimensional microvascular reconstruction