title: Identification of hybrids of painted and milky storks using FTA card-collected blood, molecular markers, and morphologies.
abstract: Suspicious hybrids of painted storks and milky storks were found in a Malaysian zoo. Blood of these birds was sampled on FTA cards for DNA fingerprinting. Of 44 optimized primers, 6 produced diagnostic markers that could identify hybrids. The markers were based on simple, direct PCR-generated multilocus banding patterns that provided two sets of genetic data, one for each of the two stork species and another for the hybrids. It also revealed that large DNA fragments (3,000 bp) could be amplified from blood collected on FTA cards. When the results of each individual bird's DNA fingerprint were compared with plumage characters, the hybrids were found to express a range of intermediate phenotypic traits of the pure breeds with no dominant plumage characteristic from either parental species.
authors.affiliation: Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, University Putra Malaysia, 43400, Serdang, Selangor, Malaysia, elsieyeeys0906@yahoo.com.
authors.forename: Elsie Yoke Sim
authors.initials: EY
authors.lastname: Yee
authors.forename: Zainal Zahari
authors.initials: ZZ
authors.lastname: Zainuddin
authors.forename: Ahmad
authors.initials: A
authors.lastname: Ismail
authors.forename: Chee Kong
authors.initials: CK
authors.lastname: Yap
authors.forename: Soon Guan
authors.initials: SG
authors.lastname: Tan
mesh_terms: D000818:Animals; D001717:Birds / Q000033:anatomy & histology / Q000145:classification* / Q000235:genetics*; D016172:DNA Fingerprinting; D017931:DNA Primers; D059788:Dried Blood Spot Testing; D005819:Genetic Markers; D006824:Hybridization, Genetic; D018895:Microsatellite Repeats; D010641:Phenotype; D016133:Polymerase Chain Reaction
chemical_list: D017931:DNA Primers; D005819:Genetic Markers