title: Determination of tiadinil and its metabolite in flue-cured tobacco.
abstract: A novel and sensitive method was developed for the determination of residues of tiadinil and its metabolite, 4-methyl-l,2,3-thiadiazole-5-carboxylic acid, in flue-cured tobacco. The pesticides were extracted with acetone and purified by gel permeation chromatography and solid-phase extraction. Analysis was performed by ultra-performance liquid chromatography-tandem mass spectrometry in negative ionization mode. Two precursor-product ion transitions were monitored for both compounds in the multiple reaction monitoring mode. Quantification was conducted by using matrix-matched standard calibration. Recovery values of the proposed method for tiadinil ranged from 72.5 to 98.2%, with relative standard deviations ranging from 3.8 to 9.5%; recovery values for 4-methyl-l,2,3-thiadiazole-5-carboxylic acid ranged from 75.4 to 103.3% with RSDs ranging from 3.7 to 9.3%. The limit of quantification for both compounds was 0.01 mg/kg. This method is valuable for residual analysis, quality control and monitoring of tiadinil and its metabolite, 4-methyl-l,2,3-thiadiazole-5-carboxylic acid, in tobacco.
authors.affiliation: Tianjin Entry & Exit Inspection and Quarantine Bureau, Tianjin 300461, P. R. China.
authors.forename: Xuyan
authors.initials: X
authors.lastname: Chen
authors.affiliation: Tianjin Entry & Exit Inspection and Quarantine Bureau, Tianjin 300461, P. R. China.
authors.forename: Kongxiang
authors.initials: K
authors.lastname: Zhao
authors.affiliation: State Key Laboratory of Elemento Organic Chemistry, Nankai University, Tianjin 300071, P. R. China chenxy04@mails.ucas.ac.cn.
authors.forename: Zhijin
authors.initials: Z
authors.lastname: Fan
authors.affiliation: State Key Laboratory of Elemento Organic Chemistry, Nankai University, Tianjin 300071, P. R. China.
authors.forename: Wutao
authors.initials: W
authors.lastname: Mao
authors.affiliation: State Key Laboratory of Elemento Organic Chemistry, Nankai University, Tianjin 300071, P. R. China.
authors.forename: Juanjuan
authors.initials: J
authors.lastname: Li
authors.affiliation: State Key Laboratory of Elemento Organic Chemistry, Nankai University, Tianjin 300071, P. R. China.
authors.forename: Xiaotian
authors.initials: X
authors.lastname: Ji
authors.affiliation: State Key Laboratory of Elemento Organic Chemistry, Nankai University, Tianjin 300071, P. R. China.
authors.forename: Xuewen
authors.initials: X
authors.lastname: Hua
authors.affiliation: State Key Laboratory of Elemento Organic Chemistry, Nankai University, Tianjin 300071, P. R. China.
authors.forename: Guangning
authors.initials: G
authors.lastname: Zong
authors.affiliation: State Key Laboratory of Elemento Organic Chemistry, Nankai University, Tianjin 300071, P. R. China.
authors.forename: Fengyun
authors.initials: F
authors.lastname: Li