title: Preparation of pancreatic acinar cells for the purpose of calcium imaging, cell injury measurements, and adenoviral infection.
abstract: The pancreatic acinar cell is the main parenchymal cell of the exocrine pancreas and plays a primary role in the secretion of pancreatic enzymes into the pancreatic duct. It is also the site for the initiation of pancreatitis. Here we describe how acinar cells are isolated from whole pancreas tissue and intracellular calcium signals are measured. In addition, we describe the techniques of transfecting these cells with adenoviral constructs, and subsequently measuring the leakage of lactate dehydrogenase, a marker of cell injury, during conditions that induce acinar cell injury in vitro. These techniques provide a powerful tool to characterize acinar cell physiology and pathology.
authors.affiliation: Rangos Research Center, Pediatric Gastroenterology, Hepatology, and Nutrition, Children's Hospital of Pittsburgh of UPMC.
authors.forename: Abrahim I
authors.initials: AI
authors.lastname: Orabi
authors.forename: Kamaldeen A
authors.initials: KA
authors.lastname: Muili
authors.forename: Dong
authors.initials: D
authors.lastname: Wang
authors.forename: Shunqian
authors.initials: S
authors.lastname: Jin
authors.forename: George
authors.initials: G
authors.lastname: Perides
authors.forename: Sohail Z
authors.initials: SZ
authors.lastname: Husain
mesh_terms: D061354:Acinar Cells / Q000166:cytology* / Q000378:metabolism*; D000256:Adenoviridae / Q000502:physiology; D000257:Adenoviridae Infections / Q000473:pathology*; D000818:Animals; D020013:Calcium Signaling; D003584:Cytological Techniques / Q000379:methods*; D008297:Male; D051379:Mice; D010179:Pancreas / Q000166:cytology* / Q000378:metabolism / Q000821:virology*