title: Mutation of OsDET1 increases chlorophyll content in rice.
abstract: As an important agronomic trait, the chlorophyll (Chl) content is closely related to photosynthesis in plants. A rice mutant Gc (Oryza sativa indica) was characterized previously by its enhanced Chl content (Chl b and total Chl) and exaggerated photosynthetic rate. Here, we describe the enhanced Chl content was caused by a mutation in the rice homolog of the DE-ETIOLATED1 (DET1) known to be involved in light transduction and morphogenesis in Arabidopsis and tomato. Sequence analysis revealed that the Gc mutant carried two fragment-insertions and a fragment-deletion upstream of the start codon of OsDET1, which led to enhance mRNA levels of OsDET1. Besides, the Gc mutant harbored a single T-to-C base transversion in the seventh exon of OsDET1, which resulted in leucine(328) to serine(328) localized in the highly conserved region. Genetic complementation demonstrated that OsDET1 mutation conferred the enhanced Chl content in the Gc mutant leaf. OsDET1 was richly expressed in green tissues, and its expression seems to be under circadian control. OsDET1-GFP fusion protein in onion epidermal cells showed that OsDET1 localized to the nucleus. These results indicated that OsDET1 mutation in Gc mutant increases Chl content in rice, which might be fundamental for enhanced photoresponsiveness.
authors.affiliation: Key Laboratory of Biorheological Science and Technology, Ministry of Education, Bioengineering College, Chongqing University, 174 Shazheng Street, Shapingba District, Chongqing 400030, China.
authors.forename: Junli
authors.initials: J
authors.lastname: Huang
authors.forename: Feng
authors.initials: F
authors.lastname: Qin
authors.forename: Guangchao
authors.initials: G
authors.lastname: Zang
authors.forename: Zhenhui
authors.initials: Z
authors.lastname: Kang
authors.forename: Hanyan
authors.initials: H
authors.lastname: Zou
authors.forename: Feng
authors.initials: F
authors.lastname: Hu
authors.forename: Caili
authors.initials: C
authors.lastname: Yue
authors.forename: Xianyong
authors.initials: X
authors.lastname: Li
authors.forename: Guixue
authors.initials: G
authors.lastname: Wang
mesh_terms: D001483:Base Sequence; D002734:Chlorophyll / Q000378:metabolism*; D002736:Chloroplasts / Q000378:metabolism; D017930:Genes, Reporter; D008027:Light; D008969:Molecular Sequence Data; D009154:Mutation; D019697:Onions / Q000166:cytology / Q000235:genetics; D009928:Organ Specificity; D012275:Oryza / Q000235:genetics* / Q000378:metabolism / Q000528:radiation effects; D010641:Phenotype; D010788:Photosynthesis; D010802:Phylogeny; D019441:Plant Epidermis / Q000235:genetics / Q000378:metabolism / Q000528:radiation effects; D018515:Plant Leaves / Q000235:genetics / Q000378:metabolism / Q000528:radiation effects; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D011993:Recombinant Fusion Proteins; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
chemical_list: D010940:Plant Proteins; D011993:Recombinant Fusion Proteins; D002734:Chlorophyll
keywords: Dark-green leaf; High chlorophyll content; OsDET1; Rice (Oryza sativa L.)