title: Alr5068, a Low-Molecular-Weight protein tyrosine phosphatase, is involved in formation of the heterocysts polysaccharide layer in the cyanobacterium Anabaena sp. PCC 7120.
abstract: The filamentous cyanobacterium Anabaena sp. PCC 7120 forms nitrogen-fixing heterocysts after deprivation of combined nitrogen. Under such conditions, vegetative cells provide heterocysts with photosynthate and receive fixed nitrogen from the latter. Heterocyst envelope contains a glycolipid layer and a polysaccharide layer to restrict the diffusion of oxygen into heterocysts. Low-Molecular-Weight protein tyrosine phosphatases (LMW-PTPs) are involved in the biosynthesis of exopolysaccharides in bacteria. Alr5068, a protein from Anabaena sp. PCC 7120, shows significant sequence similarity with LMW-PTPs. In this study we characterized the enzymatic properties of Alr5068 and showed that it can dephosphorylate several autophosphorylated tyrosine kinases (Alr2856, Alr3059 and All4432) of Anabaena sp. PCC 7120 in vitro. Several conserved residues among LMW-PTPs are shown to be essential for the phosphatase activity of Alr5068. Overexpression of alr5068 results in a strain unable to survive under diazotrophic conditions, with the formation of morphologically mature heterocysts detached from the filaments. Overexpression of an alr5068 allele that lost phosphatase activity led to the formation of heterocyst with an impaired polysaccharide layer. The alr5068 gene was upregulated after nitrogen step-down and its mutation affected the expression of hepA and hepC, two genes necessary for the formation of the heterocyst envelope polysaccharide (HEP) layer. Our results suggest that Alr5068 is associated with the production of HEP in Anabaena sp. PCC 7120.
authors.affiliation: State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, 430070 Wuhan, China. Electronic address: yanxizaoh@gmail.com.
authors.forename: Hui
authors.initials: H
authors.lastname: Tan
authors.forename: Shuang
authors.initials: S
authors.lastname: Wan
authors.forename: Pi-Qiong
authors.initials: PQ
authors.lastname: Liu
authors.forename: Li
authors.initials: L
authors.lastname: Wang
authors.forename: Cheng-Cai
authors.initials: CC
authors.lastname: Zhang
authors.forename: Wen-Li
authors.initials: WL
authors.lastname: Chen
mesh_terms: D017033:Anabaena / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D004252:DNA Mutational Analysis; D015870:Gene Expression; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial; D008970:Molecular Weight; D009586:Nitrogen Fixation; D011134:Polysaccharides / Q000378:metabolism*; D017027:Protein Tyrosine Phosphatases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D011505:Protein-Tyrosine Kinases / Q000378:metabolism
chemical_list: D011134:Polysaccharides; D011505:Protein-Tyrosine Kinases; D017027:Protein Tyrosine Phosphatases
keywords: BY-kinases; Cyanobacteria; HEP; HGL; Heterocyst nitrogen fixation; LMW-PTPs; Low-Molecular-Weight protein tyrosine phosphatases; Protein phosphorylation; Signal transduction; bacterial tyrosine kinase; heterocyst envelope glycolipid; heterocyst envelope polysaccharide