title: Host factor SAMHD1 restricts DNA viruses in non-dividing myeloid cells.
abstract: SAMHD1 is a newly identified anti-HIV host factor that has a dNTP triphosphohydrolase activity and depletes intracellular dNTP pools in non-dividing myeloid cells. Since DNA viruses utilize cellular dNTPs, we investigated whether SAMHD1 limits the replication of DNA viruses in non-dividing myeloid target cells. Indeed, two double stranded DNA viruses, vaccinia and herpes simplex virus type 1, are subject to SAMHD1 restriction in non-dividing target cells in a dNTP dependent manner. Using a thymidine kinase deficient strain of vaccinia virus, we demonstrate a greater restriction of viral replication in non-dividing cells expressing SAMHD1. Therefore, this study suggests that SAMHD1 is a potential innate anti-viral player that suppresses the replication of a wide range of DNA viruses, as well as retroviruses, which infect non-dividing myeloid cells.
authors.affiliation: Department of Microbiology and Immunology, University of Rochester, Rochester, New York, United States of America.
authors.forename: Joseph A
authors.initials: JA
authors.lastname: Hollenbaugh
authors.forename: Peter
authors.initials: P
authors.lastname: Gee
authors.forename: Jonathon
authors.initials: J
authors.lastname: Baker
authors.forename: Michele B
authors.initials: MB
authors.lastname: Daly
authors.forename: Sarah M
authors.initials: SM
authors.lastname: Amie
authors.forename: Jessica
authors.initials: J
authors.lastname: Tate
authors.forename: Natsumi
authors.initials: N
authors.lastname: Kasai
authors.forename: Yuka
authors.initials: Y
authors.lastname: Kanemura
authors.forename: Dong-Hyun
authors.initials: DH
authors.lastname: Kim
authors.forename: Brian M
authors.initials: BM
authors.lastname: Ward
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Koyanagi
authors.forename: Baek
authors.initials: B
authors.lastname: Kim
mesh_terms: D002460:Cell Line; D005260:Female; D018259:Herpesvirus 1, Human / Q000502:physiology*; D006801:Humans; D008297:Male; D020559:Monomeric GTP-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D022423:Myeloid Cells / Q000378:metabolism* / Q000473:pathology / Q000821:virology*; D000076106:SAM Domain and HD Domain-Containing Protein 1; D014616:Vaccinia virus / Q000502:physiology*; D014779:Virus Replication / Q000502:physiology*
chemical_list: D000076106:SAM Domain and HD Domain-Containing Protein 1; C484280:SAMHD1 protein, human; D020559:Monomeric GTP-Binding Proteins