title: Functional compartmental modeling of the photosystems in the thylakoid membrane at 77 K.
abstract: Time-resolved fluorescence spectroscopy measurements at 77 K on thylakoid membrane preparations and isolated photosynthetic complexes thereof were investigated using target analysis with the aim of building functional compartmental models for the photosystems in the thylakoid membrane. Combining kinetic schemes with different spectral constraints enabled us to resolve the energy transfer pathways and decay characteristics of the different emissive species. We determined the spectral and energetic properties of the red Chl pools in both photosystems and quantified the formation of LHCII-LHCI-PSI supercomplexes in the transition from native to unstacked thylakoid membranes.
authors.affiliation: Institute for Lasers, Life and Biophotonics, Faculty of Sciences, VU University Amsterdam , De Boelelaan 1081, 1081 HV, Amsterdam, The Netherlands.
authors.forename: Joris J
authors.initials: JJ
authors.lastname: Snellenburg
authors.forename: Jan P
authors.initials: JP
authors.lastname: Dekker
authors.forename: Rienk
authors.initials: R
authors.lastname: van Grondelle
authors.forename: Ivo H M
authors.initials: IH
authors.lastname: van Stokkum
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry* / Q000378:metabolism; D002734:Chlorophyll / Q000737:chemistry; D000458:Cyanobacteria / Q000378:metabolism; D004735:Energy Transfer; D007700:Kinetics; D045342:Light-Harvesting Protein Complexes / Q000737:chemistry / Q000378:metabolism; D008958:Models, Molecular; D045322:Photosynthetic Reaction Center Complex Proteins / Q000737:chemistry* / Q000378:metabolism; D045331:Photosystem I Protein Complex / Q000737:chemistry / Q000378:metabolism; D045332:Photosystem II Protein Complex / Q000737:chemistry / Q000378:metabolism; D013050:Spectrometry, Fluorescence; D018724:Spinacia oleracea / Q000378:metabolism; D013696:Temperature; D020524:Thylakoids / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D045342:Light-Harvesting Protein Complexes; D045322:Photosynthetic Reaction Center Complex Proteins; D045331:Photosystem I Protein Complex; D045332:Photosystem II Protein Complex; D002734:Chlorophyll