title: Repression of the transcription factor Bach2 contributes to predisposition of IgG1 memory B cells toward plasma cell differentiation.
abstract: Memory B cells are essential for generating rapid and robust secondary antibody responses. It has been thought that the unique cytoplasmic domain of IgG causes the prompt activation of antigen-experienced IgG memory B cells. To assess this model, we have generated a mouse containing IgG1 B cells that have never encountered antigen. We found that, upon challenge, antigen-experienced IgG1 memory B cells rapidly differentiated into plasma cells, whereas nonexperienced IgG1 B cells did not, suggesting the importance of the stimulation history. In addition, our results suggest that repression of the Bach2 transcription factor, which results from antigen experience, contributes to predisposition of IgG1 memory B cells to differentiate into plasma cells.
authors.affiliation: Laboratory for Lymphocyte Differentiation, Research Center for Allergy and Immunology, RIKEN, Yokohama, Kanagawa 230-0045, Japan.
authors.forename: Kohei
authors.initials: K
authors.lastname: Kometani
authors.forename: Rinako
authors.initials: R
authors.lastname: Nakagawa
authors.forename: Ryo
authors.initials: R
authors.lastname: Shinnakasu
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Kaji
authors.forename: Andrei
authors.initials: A
authors.lastname: Rybouchkin
authors.forename: Saya
authors.initials: S
authors.lastname: Moriyama
authors.forename: Koji
authors.initials: K
authors.lastname: Furukawa
authors.forename: Haruhiko
authors.initials: H
authors.lastname: Koseki
authors.forename: Toshitada
authors.initials: T
authors.lastname: Takemori
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Kurosaki
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D050976:Basic-Leucine Zipper Transcription Factors / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D002454:Cell Differentiation / Q000235:genetics / Q000276:immunology*; D002478:Cells, Cultured; D005434:Flow Cytometry; D015870:Gene Expression / Q000276:immunology; D007074:Immunoglobulin G / Q000276:immunology / Q000378:metabolism; D007156:Immunologic Memory / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008822:Mice, Transgenic; D051757:PAX5 Transcription Factor / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D010950:Plasma Cells / Q000276:immunology* / Q000378:metabolism; D034622:RNA Interference; D020133:Reverse Transcriptase Polymerase Chain Reaction; D058570:TOR Serine-Threonine Kinases / Q000276:immunology / Q000378:metabolism
chemical_list: C102109:Bach2 protein, mouse; D050976:Basic-Leucine Zipper Transcription Factors; D007074:Immunoglobulin G; D051757:PAX5 Transcription Factor; C497282:Pax5 protein, mouse; C546843:mTOR protein, mouse; D058570:TOR Serine-Threonine Kinases