title: Molecular characterization of a trisegmented chrysovirus isolated from the radish Raphanus sativus.
abstract: Radish (Raphanus sativus L.) is cultivated worldwide and is of agronomic importance. dsRNAs associated with partitiviruses were previously found in many R. sativus varieties. In this study, three large dsRNAs from radish were cloned using a modified single primer amplification technique. These three dsRNAs-of lengths 3638, 3517 and 3299 bp-shared conserved untranslated terminal regions, and each contained a major open reading frame putatively encoding the chrysoviral replicase, capsid protein and protease respectively. Isometric virus-like particles (VLP), approximately 45nm in diameter, were isolated from the infected radish plants. Northern blotting indicated that these dsRNAs were encapsidated in the VLP. The virus containing these dsRNA genome segments was named Raphanus sativus chrysovirus 1 (RasCV1). Phylogenetic analysis revealed that RasCV1 is a new species of the Chrysoviridae family and forms a plant taxon with another putative plant chrysovirus, Anthurium mosaic-associated virus (AmaCV). Furthermore, no fungal mycelia were observed in radish leaf tissues stained with trypan blue. These results indicated that RasCV1 is most likely a plant chrysovirus rather than a chrysovirus in symbiotic fungi. An exhaustive BLAST analysis of RasCV1 and AmaCV revealed that chrysovirus-like viruses might widely exist in eudicot and monocot plants and that endogenization of chrysovirus segments into plant genome might have ever happened.
authors.affiliation: College of Life Science, Zhejiang University, Hangzhou 310058, China.
authors.forename: Liqiang
authors.initials: L
authors.lastname: Li
authors.forename: Jianning
authors.initials: J
authors.lastname: Liu
authors.forename: Aixia
authors.initials: A
authors.lastname: Xu
authors.forename: Ting
authors.initials: T
authors.lastname: Wang
authors.forename: Jishuang
authors.initials: J
authors.lastname: Chen
authors.forename: Xiwu
authors.initials: X
authors.lastname: Zhu
mesh_terms: D003001:Cloning, Molecular; D016000:Cluster Analysis; D046529:Microscopy, Electron, Transmission; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010802:Phylogeny; D012328:RNA Viruses / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D012367:RNA, Viral / Q000235:genetics; D031224:Raphanus / Q000821:virology*; D017422:Sequence Analysis, DNA; D014764:Viral Proteins / Q000235:genetics; D014771:Virion / Q000648:ultrastructure
chemical_list: D012367:RNA, Viral; D014764:Viral Proteins
keywords: Chrysoviridae; Double-stranded RNA; Raphanus sativus; Raphanus sativus chrysovirus 1