title: One-step multiplex reverse transcription-polymerase chain reaction for the simultaneous detection of three rice viruses.
abstract: Rice stripe virus (RSV), Rice black-streaked dwarf virus (RBSDV), and Rice dwarf virus (RDV) are major rice-infecting viruses in Korea that can cause serious crop losses. A one-step multiplex reverse transcription-polymerase chain reaction (mRT-PCR) was developed for the simultaneous detection of these rice viruses. Three sets of specific primers targeted to the capsid protein coding genes of RSV, RBSDV, and RDV were used to amplify fragments that were 703 bp, 485 bp, and 252 bp, respectively. The one-step mRT-PCR assay proved to be a sensitive and rapid method for detecting the three rice viruses. This method could be used to facilitate better control of rice viruses.
authors.affiliation: National Institute of Crop Science, RDA, Suwon, Republic of Korea.
authors.forename: Sang-Yun
authors.initials: SY
authors.lastname: Cho
authors.forename: Rae-Dong
authors.initials: RD
authors.lastname: Jeong
authors.forename: Young-Nam
authors.initials: YN
authors.lastname: Yoon
authors.forename: Su-Heon
authors.initials: SH
authors.lastname: Lee
authors.forename: Dong Bum
authors.initials: DB
authors.lastname: Shin
authors.forename: Hang-Won
authors.initials: HW
authors.lastname: Kang
authors.forename: Bong Choon
authors.initials: BC
authors.lastname: Lee
mesh_terms: D036022:Capsid Proteins / Q000235:genetics; D017931:DNA Primers / Q000235:genetics; D007723:Korea; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*; D012275:Oryza / Q000821:virology*; D010935:Plant Diseases / Q000821:virology*; D012087:Reoviridae / Q000235:genetics / Q000302:isolation & purification*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D029364:Tenuivirus / Q000235:genetics / Q000302:isolation & purification*; D013997:Time Factors; D014773:Virology / Q000379:methods
chemical_list: D036022:Capsid Proteins; D017931:DNA Primers
keywords: One-step multiplex RT-PCR; Rice black-streaked dwarf virus; Rice dwarf virus; Rice stripe virus; Simultaneous detection