title: Semi-quantitative analysis of transcript accumulation in response to drought stress by Lepidium latifolium seedlings.
abstract: Cross-amplification of five Arabidopsis abiotic stress-responsive genes (AtPAP, ZFAN, Vn, LC4 and SNS) in Lepidium has been documented in plants raised out of seeds pre-treated with potassium nitrate (KNO 3) for assessment of enhanced drought stress tolerance. cDNA was synthesized from Lepidium plants pre-treated with KNO 3 (0.1% and 0.3%) and exposed to drought conditions (5% and 15% PEG) at seedling stage for 30 d. Transcript accumulation of all the five genes were found suppressed in set of seedlings, which were pre-treated with 0.1% KNO 3 and were exposed to 15% PEG for 30 d. The present study establishes that different pre-treatments may further enhance the survivability of Lepidium plants under conditions of drought stress to different degrees.
authors.affiliation: Molecular Biology and Genetic Engineering Laboratory; Defence Institute of Bio-Energy Research; DRDO; Haldwani, India; These authors contributed equally to this work.
authors.forename: Sanjay Mohan
authors.initials: SM
authors.lastname: Gupta
authors.forename: Sadhana
authors.initials: S
authors.lastname: Singh
authors.forename: Pankaj
authors.initials: P
authors.lastname: Pandey
authors.forename: Atul
authors.initials: A
authors.lastname: Grover
authors.forename: Zakwan
authors.initials: Z
authors.lastname: Ahmed
mesh_terms: D055864:Droughts; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D018525:Germination / Q000235:genetics; D029686:Lepidium / Q000235:genetics* / Q000502:physiology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D036226:Seedlings / Q000235:genetics* / Q000502:physiology*; D012639:Seeds / Q000235:genetics; D013312:Stress, Physiological / Q000235:genetics*
chemical_list: D012333:RNA, Messenger
keywords: Lepidium latifolium; drought stress; polyethylene glycol; pre-germination treatment; seed dormancy