title: pstS1 polymorphisms of Mycobacterium tuberculosis strains may reflect ongoing immune evasion.
abstract: The mycobacterial antigen PstS1 is a highly immunogenic and immunostimulatory component of the mycobacterial cell membrane and a good candidate for the diagnosis and vaccination against tuberculosis. Here we selected 180 clinical isolates of Mycobacterium tuberculosis complex (MTBC) in China and 11 different Bacille Calmette Guerin (BCG) strains, amplified the gene of the PstS1 antigen and compared the sequences with those of four other Mycobacterium bovis and BCG strains from the NCBI genome website. Some of the mutations, especially 2 frameshift mutations, occurred in the PstS1antigen, which may have resulted in the protein function alteration and ongoing immune evasion. A unique single nucleotide polymorphism of the M. bovis and BCG strains was found in this antigen and may be useful for differentiating M. bovis and BCG strains from M. tuberculosis strains.
authors.affiliation: National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, State Key Laboratory for Infectious Disease Prevention and Control, Beijing 102206, PR China. liuhaican@126.com
authors.forename: Haican
authors.initials: H
authors.lastname: Liu
authors.forename: Yi
authors.initials: Y
authors.lastname: Jiang
authors.forename: Xiangfeng
authors.initials: X
authors.lastname: Dou
authors.forename: Haiyin
authors.initials: H
authors.lastname: Wang
authors.forename: Xiuqin
authors.initials: X
authors.lastname: Zhao
authors.forename: Wen
authors.initials: W
authors.lastname: Zhang
authors.forename: Li
authors.initials: L
authors.lastname: Wan
authors.forename: Zhijian
authors.initials: Z
authors.lastname: Zhang
authors.forename: Chen
authors.initials: C
authors.lastname: Chen
authors.forename: Kanglin
authors.initials: K
authors.lastname: Wan
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000235:genetics* / Q000378:metabolism; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D015373:Bacterial Typing Techniques / Q000379:methods; D018984:Epitopes, T-Lymphocyte / Q000235:genetics; D006801:Humans; D057131:Immune Evasion / Q000235:genetics*; D008969:Molecular Sequence Data; D009154:Mutation; D009163:Mycobacterium bovis / Q000145:classification / Q000235:genetics; D009169:Mycobacterium tuberculosis / Q000145:classification / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D020641:Polymorphism, Single Nucleotide; D016415:Sequence Alignment
chemical_list: D018528:ATP-Binding Cassette Transporters; D001426:Bacterial Proteins; D018984:Epitopes, T-Lymphocyte; C581205:PstS-1 protein, Mycobacterium tuberculosis
keywords: Immune evasion; Mycobacterium tuberculosis complex; PstS1 polymorphisms