title: Translational repression using BIV Tat peptide-TAR RNA interaction in mammalian cells.
abstract: We developed a strategy to create novel genetically encoded switches based on translational repression. We illustrated its efficacy by incorporating two copies of an RNA hairpin in the 5'-untranslated region (UTR) of a target mRNA and demonstrating 7-fold translational repression upon expression of a ligand - the BIV Tat peptide.
authors.affiliation: Department of Chemical and Biological Engineering, Rensselaer Polytechnic Institute, Troy, New York 12180, USA.
authors.forename: Chaitanya
authors.initials: C
authors.lastname: Sudrik
authors.forename: Manish
authors.initials: M
authors.lastname: Arha
authors.forename: Jicong
authors.initials: J
authors.lastname: Cao
authors.forename: David V
authors.initials: DV
authors.lastname: Schaffer
authors.forename: Ravi S
authors.initials: RS
authors.lastname: Kane
mesh_terms: D020121:5' Untranslated Regions; D000818:Animals; D002460:Cell Line; D005786:Gene Expression Regulation; D016087:Immunodeficiency Virus, Bovine / Q000235:genetics*; D010455:Peptides / Q000235:genetics*; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000737:chemistry / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics; D014764:Viral Proteins / Q000235:genetics*
chemical_list: D020121:5' Untranslated Regions; D010455:Peptides; D012333:RNA, Messenger; D012367:RNA, Viral; D014764:Viral Proteins