title: Glyphosate effects on the gene expression of the apical bud in soybean (Glycine max).
abstract: Glyphosate is a broad spectrum, non-selective herbicide which has been widely used for weed control. Much work has focused on elucidating the high accumulation of glyphosate in shoot apical bud (shoot apex). However, to date little is known about the molecular mechanisms of the sensitivity of shoot apical bud to glyphosate. Global gene expression profiling of the soybean apical bud response to glyphosate treatment was performed in this study. The results revealed that the glyphosate inhibited tryptophan biosynthesis of the shikimic acid pathway in the soybean apical bud, which was the target site of glyphosate. Glyphosate inhibited the expression of most of the target herbicide site genes. The promoter sequence analysis of key target genes revealed that light responsive elements were important regulators in glyphosate induction. These results will facilitate further studies of cloning genes and molecular mechanisms of glyphosate on soybean shoot apical bud.
authors.affiliation: The National Key Facility for Crop Gene Resources and Genetic Improvement/Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, PR China.
authors.forename: Ling-Xue
authors.initials: LX
authors.lastname: Jiang
authors.forename: Long-Guo
authors.initials: LG
authors.lastname: Jin
authors.forename: Yong
authors.initials: Y
authors.lastname: Guo
authors.forename: Bo
authors.initials: B
authors.lastname: Tao
authors.forename: Li-Juan
authors.initials: LJ
authors.lastname: Qiu
mesh_terms: D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D005998:Glycine / Q000031:analogs & derivatives* / Q000494:pharmacology; D006540:Herbicides / Q000494:pharmacology; D008027:Light; D018520:Plant Shoots / Q000187:drug effects*; D011401:Promoter Regions, Genetic; D012765:Shikimic Acid / Q000378:metabolism; D013025:Glycine max / Q000187:drug effects / Q000235:genetics*; D014364:Tryptophan / Q000096:biosynthesis; D000097797:Glyphosate
chemical_list: D005998:Glycine; D006540:Herbicides; D012765:Shikimic Acid; D014364:Tryptophan; D000097797:Glyphosate
keywords: 12 SHAPs; 12 genes participated in shikimate acid pathway; 26 DGEs; 26 differentially expressed cross talk genes at three time points; DGE; Digital gene expression profiling; FC; FDR; GO; Glyphosate; KEGG; Shoot apical bud; Soybean; cDNA; complementary deoxyribonucleic acid; digital gene expression tag profiling; false detection rate; fold change; gene ontology; kyoto encyclopedia of genes and genomes