title: A cell wall recycling shortcut that bypasses peptidoglycan de novo biosynthesis.
abstract: We report a salvage pathway in Gram-negative bacteria that bypasses de novo biosynthesis of UDP N-acetylmuramic acid (UDP-MurNAc), the first committed peptidoglycan precursor, and thus provides a rationale for intrinsic fosfomycin resistance. The anomeric sugar kinase AmgK and the MurNAc α-1-phosphate uridylyl transferase MurU, defining this new cell wall sugar-recycling route in Pseudomonas putida, were characterized and engineered into Escherichia coli, channeling external MurNAc directly to peptidoglycan biosynthesis.
authors.affiliation: Department of Biology, Konstanz Research School Chemical Biology, University of Konstanz, Konstanz, Germany.
authors.forename: Jonathan
authors.initials: J
authors.lastname: Gisin
authors.forename: Alexander
authors.initials: A
authors.lastname: Schneider
authors.forename: Bettina
authors.initials: B
authors.lastname: Nägele
authors.forename: Marina
authors.initials: M
authors.lastname: Borisova
authors.forename: Christoph
authors.initials: C
authors.lastname: Mayer
mesh_terms: D002473:Cell Wall / Q000737:chemistry / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology / Q000378:metabolism; D010457:Peptidoglycan / Q000096:biosynthesis* / Q000737:chemistry; D016958:Pseudomonas putida / Q000166:cytology / Q000201:enzymology / Q000378:metabolism*
chemical_list: D010457:Peptidoglycan