title: Methylene blue protects against paraquat-induced acute lung injury in rats.
abstract: Methylene blue (MB) attenuates acute lung injury (ALI) induced by liver or pancreas ischemia-reperfusion. But the protective effects of MB on paraquat (PQ)-induced ALI have not been elucidated. In this study, we aimed to study the effects of MB on ALI caused by PQ in rats. Male Sprague-Dawley rats were challenged acutely by PQ (25mg/kg, i.p.) with or without MB posttreatment (2mg/kg, i.p.) 2h after PQ administration. Lung specimens and the bronchoalveolar lavage fluid were isolated for pathological examinations and biochemical analyses 24h after PQ exposure. We found that the administration of MB significantly increased the activity of superoxide dismutase and decreased lung wet dry radio, the content of malondialdehyde and lactate dehydrogenase, the total cell number and the level of myeloperoxidase in bronchoalveolar lavage fluid. Pathological examination also revealed that MB alleviated PQ-induced histological damage. These results provide evidence that MB may be a new therapy in the treatment of PQ-induced ALI.
authors.affiliation: Xuzhou Prevention and Treatment Hospital of Occupational Diseases, Xuzhou, Jiangsu, China.
authors.forename: Zhi-guang
authors.initials: ZG
authors.lastname: Tian
authors.forename: Yong
authors.initials: Y
authors.lastname: Ji
authors.forename: Wen-jing
authors.initials: WJ
authors.lastname: Yan
authors.forename: Chuang-ying
authors.initials: CY
authors.lastname: Xu
authors.forename: Qing-yan
authors.initials: QY
authors.lastname: Kong
authors.forename: Feng
authors.initials: F
authors.lastname: Han
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhao
authors.forename: Qing-feng
authors.initials: QF
authors.lastname: Pang
mesh_terms: D055371:Acute Lung Injury / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism; D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology / Q000276:immunology*; D002478:Cells, Cultured; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008168:Lung / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D008751:Methylene Blue / Q000008:administration & dosage*; D018384:Oxidative Stress / Q000187:drug effects; D010269:Paraquat / Q000633:toxicity*; D009195:Peroxidase / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D008315:Malondialdehyde; D007770:L-Lactate Dehydrogenase; D009195:Peroxidase; D013482:Superoxide Dismutase; D010269:Paraquat; D008751:Methylene Blue
keywords: Acute lung injury; Methylene blue; Paraquat