title: Milk from Brazilian women presents secretory IgA antibodies and neutralizes rotavirus G9P[5].
abstract: OBJECTIVE
  To verify the presence of anti-rotavirus serotype G9P[5] SIgA and the virus neutralization capacity of milk samples from Brazilian women.
  
  
  METHODS
  SIgA antibody levels reactive to rotavirus G9 were determined in 30 maternal milk samples by enzyme-linked immunosorbent assay (ELISA) using purified virus suspensions. The samples' capacity to neutralize rotavirus G9P[5] was analyzed using the MA-104 cells neutralization assay.
  
  
  RESULTS
  Great individual variations were observed regarding the SIgA levels and neutralization titers, but all samples showed some G9P[5] neutralizing ability. A highly significant positive correlation was observed between antibody levels and neutralization titers.
  
  
  CONCLUSIONS
  The high correlation between anti-rotavirus antibody levels and neutralizing capacity of the milk samples suggests a possible protective role of these antibodies against infection. These results also support the encouragement of the breast-feeding practice.
authors.affiliation: MD. Laboratório de Imunogenética, Instituto Butantan, São Paulo, SP, Brazil.
authors.forename: Simone M R
authors.initials: SM
authors.lastname: Santos
authors.forename: Thalita L
authors.initials: TL
authors.lastname: Ferreira
authors.forename: Virgínia S
authors.initials: VS
authors.lastname: Quintal
authors.forename: Solange B
authors.initials: SB
authors.lastname: Carbonare
authors.forename: Milene
authors.initials: M
authors.lastname: Tino-de-Franco
mesh_terms: D000328:Adult; D057134:Antibodies, Neutralizing / Q000502:physiology*; D000914:Antibodies, Viral / Q000276:immunology*; D001942:Breast Feeding; D002460:Cell Line / Q000821:virology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007071:Immunoglobulin A, Secretory / Q000737:chemistry*; D008895:Milk, Human / Q000276:immunology* / Q000821:virology; D009500:Neutralization Tests / Q000379:methods; D012401:Rotavirus / Q000145:classification / Q000276:immunology* / Q000302:isolation & purification; D012703:Serotyping; D018709:Statistics, Nonparametric; D055815:Young Adult
chemical_list: D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D007071:Immunoglobulin A, Secretory
keywords: Human milk; IgA secretora; Leite humano; Neutralização do rotavírus; Rotavirus; Rotavirus neutralization; Rotavírus; Secretory IgA