title: Expression of microRNA-1234 related signal transducer and activator of transcription 3 in patients with diffuse large B-cell lymphoma of activated B-cell like type from high and low infectious disease areas.
abstract: Abstract Diffuse large B-cell lymphoma (DLBCL) is a heterogeneous disease, and infectious agents are suspected to be involved in the tumorigenesis of DLBCL. MicroRNAs (miRNAs) are non-coding RNAs modulating protein expression. We compared miRNA expression profiles in lymph node tissues of patients with DLBCL of the activated B-cell like (ABC) type from two geographical areas with different background exposures, Sweden and Egypt. We showed previously that DLBCL tissues of the ABC-type in Swedish patients had a higher expression of signal transducer and activator of transcription 3 (STAT3) compared to Egyptian patients. Here, we analyzed the involvement of miRNAs in STAT3 regulation. miR-1234 was significantly up-regulated in Egyptian patients with DLBCL compared to Swedish patients (p < 0.03). The miR-1234 expression level correlated inversely with the expression of STAT3. The Stat3 protein was down-regulated in cells transfected with miR-1234, suggesting that STAT3 might be a potential target for miR-1234. miR-1234 and STAT3 might be involved in the tumorigenesis of DLBCL of ABC type and possibly associated with environmental background exposure.
authors.affiliation: Department of Oncology and Pathology, Karolinska Institutet , Stockholm , Sweden.
authors.forename: Therese
authors.initials: T
authors.lastname: Högfeldt
authors.forename: Per
authors.initials: P
authors.lastname: Johnsson
authors.forename: Dan
authors.initials: D
authors.lastname: Grandér
authors.forename: Abeer A
authors.initials: AA
authors.lastname: Bahnassy
authors.forename: Anna
authors.initials: A
authors.lastname: Porwit
authors.forename: Salem
authors.initials: S
authors.lastname: Eid
authors.forename: Anders
authors.initials: A
authors.lastname: Österborg
authors.forename: Abdel-Rahman N
authors.initials: AR
authors.lastname: Zekri
authors.forename: Joachim
authors.initials: J
authors.lastname: Lundahl
authors.forename: Mustafa Hussein
authors.initials: MH
authors.lastname: Khaled
authors.forename: Håkan
authors.initials: H
authors.lastname: Mellstedt
authors.forename: Ali
authors.initials: A
authors.lastname: Moshfegh
mesh_terms: D000328:Adult; D000368:Aged; D016022:Case-Control Studies; D002460:Cell Line; D016000:Cluster Analysis; D004534:Egypt; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D016403:Lymphoma, Large B-Cell, Diffuse / Q000188:drug therapy / Q000235:genetics* / Q000378:metabolism / Q000401:mortality; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D034622:RNA Interference; D050796:STAT3 Transcription Factor / Q000235:genetics* / Q000378:metabolism; D013548:Sweden; D059467:Transcriptome; D014162:Transfection
chemical_list: C000593631:MIRN-1234 microRNA, human; D035683:MicroRNAs; D050796:STAT3 Transcription Factor