title: Association of cytotoxic T-lymphocyte antigen 4 genetic polymorphism, hepatitis C viral infection and B-cell non-Hodgkin lymphoma: an Egyptian study.
abstract: Abstract Genetic and environmental factors are involved in the pathogenesis of non-Hodgkin lymphoma (NHL). The present study aimed to investigate the association between cytotoxic T-lymphocyte antigen 4 (CTLA-4) genetic polymorphism, hepatitis C virus (HCV) infection and B-cell NHL risk in Egypt. Genotyping of CTLA-4 single nucleotide polymorphisms (SNPs) was performed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay for 181 adult patients with B-NHL and 200 controls. Our study revealed that CTLA-4 + 49 A/G polymorphism conferred increased risk of B-NHL (odds ratio [OR] = 1.7, 95% confidence interval [CI] = 1.36-2.565). The prevalence of HCV infection in individuals harboring the mutant genotype + 49 A/G and - 318 C/T SNPs was higher in patients with B-NHL and was associated with increased risk of B-NHL (OR = 2.79, 95% CI = 1.24-6.93 for + 49 A/G and OR = 3.9, 95% CI = 1.01-15.98 for - 318 C/T). In conclusion, some SNPs of CTLA-4 are genetic risk factors for B-NHL. Moreover, this study identified an association of CTLA-4 + 49 A/G and - 318 C/T promoter polymorphisms with HCV infection.
authors.affiliation: Department of Clinical and Chemical Pathology, Faculty of Medicine, Cairo University , Cairo , Egypt.
authors.forename: Mervat Mamdooh
authors.initials: MM
authors.lastname: Khorshied
authors.forename: Heba Mahmoud
authors.initials: HM
authors.lastname: Gouda
authors.forename: Ola M Reda
authors.initials: OM
authors.lastname: Khorshid
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000483:Alleles; D060908:CTLA-4 Antigen / Q000235:genetics*; D016022:Case-Control Studies; D004534:Egypt; D005091:Exons; D005260:Female; D056726:Genetic Association Studies; D005838:Genotype; D006526:Hepatitis C / Q000150:complications*; D006801:Humans; D016393:Lymphoma, B-Cell / Q000150:complications* / Q000235:genetics* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009362:Neoplasm Metastasis; D009367:Neoplasm Staging; D016017:Odds Ratio; D011110:Polymorphism, Genetic; D020641:Polymorphism, Single Nucleotide; D055815:Young Adult
chemical_list: D060908:CTLA-4 Antigen