title: Maternal antibody induced by recombinant gp85 protein vaccine adjuvanted with CpG-ODN protects against ALV-J early infection in chickens.
abstract: In this study, the efficacy of a recombinant protein vaccine encoding the gp85 gene from the subgroup J avian leukosis virus (ALV-J) co-administered with cytosine-phosphate-guanine oligodeoxynucleotide (CpG-ODN) or Freund's adjuvants was investigated for the protection against early ALV-J infection in chickens. The gp85 gene from ALV-J was amplified using polymerase chain reaction (PCR), and the recombinant protein was expressed in Escherichia coli. The purified recombinant protein was injected intramuscularly into the breeder hens along with CpG-ODN or Freund's adjuvants, and the antibodies in the serum were assayed regularly post inoculation. The fertilized eggs from the vaccinated hens were hatched, the hatched chickens were challenged with 10(2.2) 50% tissue culture infective dose (TCID50) ALV-J on 1 day, and the maternal antibodies in the hatched chickens were examined regularly before and after the challenge. The viremia was determined weekly, and a histopathological analysis of the immunosuppressive lesions was performed. The results suggest that the gp85 recombinant protein was successfully prepared and was inoculated with CpG-ODN adjuvant into breeder hens to induce serological antibody against ALV-J in the hens and in the hatched chickens. The positive maternal antibodies in the hatched chickens provided effective protection for most chickens against viremia and dramatically decreased the number of immunosuppressive lesions; these protective effects were better than those of the gp85 recombinant protein plus Freund's adjuvant. The data will provide a scientific basis for the application of the ALV-J subunit vaccine to control ALV-J infection in chicken flocks.
authors.affiliation: Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai'an 271018, PR China.
authors.forename: Wenwen
authors.initials: W
authors.lastname: Dou
authors.forename: Hongmei
authors.initials: H
authors.lastname: Li
authors.forename: Ziqiang
authors.initials: Z
authors.lastname: Cheng
authors.forename: Peng
authors.initials: P
authors.lastname: Zhao
authors.forename: Jianzhu
authors.initials: J
authors.lastname: Liu
authors.forename: Zhizhong
authors.initials: Z
authors.lastname: Cui
authors.forename: Haigang
authors.initials: H
authors.lastname: Liu
authors.forename: Weifang
authors.initials: W
authors.lastname: Jing
authors.forename: Huijun
authors.initials: H
authors.lastname: Guo
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage*; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D001353:Avian Leukosis / Q000276:immunology / Q000473:pathology / Q000517:prevention & control* / Q000821:virology; D001354:Avian Leukosis Virus / Q000235:genetics / Q000276:immunology*; D002645:Chickens; D004926:Escherichia coli / Q000235:genetics; D005620:Freund's Adjuvant / Q000008:administration & dosage; D015870:Gene Expression; D006651:Histocytochemistry; D007112:Immunity, Maternally-Acquired; D007273:Injections, Intramuscular; D009838:Oligodeoxyribonucleotides / Q000008:administration & dosage*; D022223:Vaccines, Subunit / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D014764:Viral Proteins / Q000235:genetics / Q000276:immunology; D014765:Viral Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D014766:Viremia / Q000517:prevention & control
chemical_list: D000276:Adjuvants, Immunologic; D000914:Antibodies, Viral; C408982:CPG-oligonucleotide; D009838:Oligodeoxyribonucleotides; D022223:Vaccines, Subunit; D014614:Vaccines, Synthetic; D014764:Viral Proteins; D014765:Viral Vaccines; D005620:Freund's Adjuvant
keywords: CpG-ODN; Immunoprotection; Maternal antibody; Recombinant gp85 protein; Subgroup J avian leukosis virus (ALV-J)