title: Separation and purification of five phenylpropanoid glycosides from Lamiophlomis rotata (Benth.) Kudo by a macroporous resin column combined with high-speed counter-current chromatography.
abstract: Five phenylethanoid glycosides (PhGs), forsythoside B, verbascoside, alyssonoside, isoverbascoside, and leucosceptoside B, were isolated and purified from Lamiophlomis rotata (Benth.) Kudo by high-speed counter-current chromatography (HSCCC) combined with macroporous resin (MR) column separation. In the present study, the two-phase solvent system composed of ethyl acetate/n-butanol/water (13:3:10, v/v/v) was used for HSCCC separation. A total of 27 mg of forsythoside B, 41 mg of verbascoside, 29 mg of alyssonoside, 23 mg of isoverbascoside, and 13 mg of leucosceptoside B with purities of 97.7, 99.2, 99.5, 99.3, and 97.3%, respectively, were obtained in a one-step separation within 4 h from 150 mg of crude extract. The recoveries of the five PhGs after MR-HSCCC separation were 74.5, 76.5, 72.5, 76.4, and 77.0%, respectively. The chemical structures of all five compounds were identified by (1) H and (13) C NMR spectroscopy.
authors.affiliation: Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, China.
authors.forename: Hui-Lan
authors.initials: HL
authors.lastname: Yue
authors.forename: Xiao-Hui
authors.initials: XH
authors.lastname: Zhao
authors.forename: Li-Juan
authors.initials: LJ
authors.lastname: Mei
authors.forename: Yun
authors.initials: Y
authors.lastname: Shao
mesh_terms: D003377:Countercurrent Distribution; D006027:Glycosides / Q000737:chemistry / Q000302:isolation & purification*; D019686:Lamiaceae / Q000737:chemistry*; D010666:Phenylpropionates / Q000737:chemistry / Q000302:isolation & purification*; D016062:Porosity; D012117:Resins, Synthetic / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D006027:Glycosides; D010666:Phenylpropionates; D012117:Resins, Synthetic
keywords: HSCCC; Lamiophlomis rotata (Benth.) Kudo; Macroporous resins; Phenylpropanoid glycosides; Traditional Chinese medicines