title: Methylglyoxal further impairs adipose tissue metabolism after partial decrease of blood supply.
abstract: We previously showed that methylglyoxal-induced glycation induces adipose tissue lesions, including decreased irrigation and macrophage recruitment, independently of obesity. Here, we developed a model of partially decreased adipose tissue irrigation, a common condition in obese individuals. We aimed to study the role of methylglyoxal in the metabolic adaptations to such conditions 1 and 48 hours after decreased blood supply, avoiding other confoundable variables. Irrigation decrease during 1 hour leaded to increased activation of ERK1/2 and degradation of Ikappa-Balpha and Perilipin A in methylglyoxal-treated normal Wistar rats. After 48 hours, all rats showed increased fasting glycaemia and insulinemia. However, methylglyoxal-treated rats had higher free fatty acids and triglycerides levels and decreased adiponectinemia, consequent to decreased PPARgamma levels in partially irrigated adipose tissue. Our data show that besides causing vascular dysfunction, glycation further contributes to impaired adipocyte metabolism after a decrease of tissue irrigation, what may hamper metabolic adaptation during tissue expansion.
authors.affiliation: Laboratory of Physiology, Institute of Biomedical Imaging and Life Sciences (IBILI), Faculty of Medicine, University of Coimbra , Portugal.
authors.forename: Tiago
authors.initials: T
authors.lastname: Rodrigues
authors.forename: Paulo
authors.initials: P
authors.lastname: Matafome
authors.forename: Raquel
authors.initials: R
authors.lastname: Seiça
mesh_terms: D000273:Adipose Tissue / Q000098:blood supply* / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D004822:Epididymis / Q000166:cytology; D006031:Glycosylation / Q000187:drug effects; D007511:Ischemia / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D008297:Male; D011183:Postoperative Complications / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D011765:Pyruvaldehyde / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D015398:Signal Transduction / Q000187:drug effects; D013997:Time Factors
chemical_list: D015415:Biomarkers; D011765:Pyruvaldehyde