title: Triple negative breast cancers have a reduced expression of DNA repair genes.
abstract: DNA repair is a key determinant in the cellular response to therapy and tumor repair status could play an important role in tailoring patient therapy. Our goal was to evaluate the mRNA of 13 genes involved in different DNA repair pathways (base excision, nucleotide excision, homologous recombination, and Fanconi anemia) in paraffin embedded samples of triple negative breast cancer (TNBC) compared to luminal A breast cancer (LABC). Most of the genes involved in nucleotide excision repair and Fanconi Anemia pathways, and CHK1 gene were significantly less expressed in TNBC than in LABC. PARP1 levels were higher in TNBC than in LABC. In univariate analysis high level of FANCA correlated with an increased overall survival and event free survival in TNBC; however multivariate analyses using Cox regression did not confirm FANCA as independent prognostic factor. These data support the evidence that TNBCs compared to LABCs harbour DNA repair defects.
authors.affiliation: Laboratório de Citogenética Humana e Oncogenética, Departamento de Genética, UFPR, Curitiba, Paraná, Brazil.
authors.forename: Enilze
authors.initials: E
authors.lastname: Ribeiro
authors.forename: Monica
authors.initials: M
authors.lastname: Ganzinelli
authors.forename: Daniele
authors.initials: D
authors.lastname: Andreis
authors.forename: Ramona
authors.initials: R
authors.lastname: Bertoni
authors.forename: Roberto
authors.initials: R
authors.lastname: Giardini
authors.forename: Stephen B
authors.initials: SB
authors.lastname: Fox
authors.forename: Massimo
authors.initials: M
authors.lastname: Broggini
authors.forename: Alberto
authors.initials: A
authors.lastname: Bottini
authors.forename: Vanessa
authors.initials: V
authors.lastname: Zanoni
authors.forename: Letizia
authors.initials: L
authors.lastname: Bazzola
authors.forename: Chiara
authors.initials: C
authors.lastname: Foroni
authors.forename: Daniele
authors.initials: D
authors.lastname: Generali
authors.forename: Giovanna
authors.initials: G
authors.lastname: Damia
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D004260:DNA Repair / Q000235:genetics*; D015536:Down-Regulation; D005199:Fanconi Anemia / Q000235:genetics; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D059765:Homologous Recombination / Q000235:genetics; D006801:Humans; D008875:Middle Aged; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011960:Receptors, Estrogen / Q000378:metabolism; D064726:Triple Negative Breast Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology
chemical_list: D012333:RNA, Messenger; D011960:Receptors, Estrogen