title: Development of chemical probes for biochemical detection and cellular imaging of myristoylated and palmitoylated proteins.
abstract: Fatty acylation of proteins regulates their spatial localization and activity in living cells. Methods to monitor fatty acylation are invaluable for studying its role in regulating protein dynamics. The protocols in this unit describe a procedure that involves metabolic labeling with ω-alkynyl fatty acids for detecting and cellular imaging of fatty-acylated proteins, namely myristoylated and palmitoylated proteins. Curr. Protoc. Chem. Biol. 3:15-26 © 2011 by John Wiley & Sons, Inc.
authors.affiliation: Genentech, South San Francisco, California.
authors.forename: Rami N
authors.initials: RN
authors.lastname: Hannoush
keywords: alkynyl fatty acids; chemical probes; click chemistry; fatty acylation; metabolic labeling; myristoylation; palmitoylation