title: Interlaboratory comparison of intact-cell matrix-assisted laser desorption ionization-time of flight mass spectrometry results for identification and differentiation of Brucella spp.
abstract: Classical microbiological diagnosis of human brucellosis is time-consuming, hazardous, and subject to variable interpretation. Intact-cell matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) was evaluated for the routine identification of Brucella spp. Analysis of mass peak patterns allowed accurate identification to the genus level. However, statistical models based on peak intensities were needed for definite species differentiation. Interlaboratory comparison confirmed the reproducibility of the results.
authors.affiliation: Friedrich-Loeffler-Institut, Institute of Molecular Biology, Greifswald-Insel Riems, Germany.
authors.forename: Axel
authors.initials: A
authors.lastname: Karger
authors.forename: Falk
authors.initials: F
authors.lastname: Melzer
authors.forename: Markus
authors.initials: M
authors.lastname: Timke
authors.forename: Barbara
authors.initials: B
authors.lastname: Bettin
authors.forename: Markus
authors.initials: M
authors.lastname: Kostrzewa
authors.forename: Karsten
authors.initials: K
authors.lastname: Nöckler
authors.forename: Angelika
authors.initials: A
authors.lastname: Hohmann
authors.forename: Herbert
authors.initials: H
authors.lastname: Tomaso
authors.forename: Heinrich
authors.initials: H
authors.lastname: Neubauer
authors.forename: Sascha
authors.initials: S
authors.lastname: Al Dahouk
mesh_terms: D002002:Brucella / Q000737:chemistry / Q000145:classification* / Q000302:isolation & purification*; D002006:Brucellosis / Q000175:diagnosis* / Q000382:microbiology*; D006801:Humans; D015203:Reproducibility of Results; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*