title: Transmissible gastroenteritis virus: identification of M protein-binding peptide ligands with antiviral and diagnostic potential.
abstract: The membrane (M) protein is one of the major structural proteins of coronavirus particles. In this study, the M protein of transmissible gastroenteritis virus (TGEV) was used to biopan a 12-mer phage display random peptide library. Three phages expressing TGEV-M-binding peptides were identified and characterized in more depth. A phage-based immunosorbent assay (phage-ELISA) capable of differentiating TGEV from other coronaviruses was developed using one phage, phTGEV-M7, as antigen. When the phage-ELISA was compared to conventional antibody-based ELISA for detecting infections, phage-ELISA exhibited greater sensitivity. A chemically synthesized, TGEV-M7 peptide (pepTGEV-M7; HALTPIKYIPPG) was evaluated for antiviral activity. Plaque-reduction assays revealed that pepTGEV-M7 was able to prevent TGEV infection in vitro (p<0.01) following pretreatment of the virus with the peptide. Indirect immunofluorescence and real-time RT-PCR confirmed the inhibitory effects of the peptide. These results indicate that pepTGEV-M7 might be utilized for virus-specific diagnostics and treatment.
authors.affiliation: Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, 59 Mucai Street, Xiangfang District, Harbin 150030, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Zou
authors.forename: Dante S
authors.initials: DS
authors.lastname: Zarlenga
authors.forename: Karol
authors.initials: K
authors.lastname: Sestak
authors.forename: Siqingaowa
authors.initials: S
authors.lastname: Suo
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Ren
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000998:Antiviral Agents / Q000737:chemistry / Q000494:pharmacology; D000086862:Coronavirus M Proteins; D005761:Gastroenteritis, Transmissible, of Swine / Q000175:diagnosis / Q000188:drug therapy / Q000821:virology*; D008024:Ligands; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D013552:Swine; D005760:Transmissible gastroenteritis virus / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D014763:Viral Matrix Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D000998:Antiviral Agents; D000086862:Coronavirus M Proteins; D008024:Ligands; C000706238:M protein, Transmissible gastroenteritis virus; D010455:Peptides; D014763:Viral Matrix Proteins
keywords: Antivirals; Membrane protein; Peptide; TGEV