title: Mapping the redox state of CHOP-treated non-Hodgkin's lymphoma xenografts in mice.
abstract: Drug treatment may alter the metabolism of cancer cells and may alter the mitochondrial redox state. Using the redox scanner that collects the fluorescence signals from both the oxidized flavoproteins (Fp) and the reduced form of nicotinamide adenine dinucleotide (NADH) in snap-frozen tumor tissues, we investigated the effects of chemotherapy on mouse xenografts of a human diffuse large B-cell lymphoma cell line (DLCL2). The mice in the treatment group were treated with CHOP - cyclophosphamide (C) + hydroxydoxorubicin (H) + Oncovin (O) + prednisone (P) using the following regimen: CHO administration on day 1 followed by prednisone administration on day 1-5. On day 5 the mitochondrial redox state of the treated group was slightly more reduced than that of the control group (p = 0.049), and the Fp content of the treated group was significantly decreased (p = 0.033).
authors.affiliation: Department of Radiology, University of Pennsylvania, Philadelphia, PA, USA.|Britton Chance Laboratory of Redox Imaging, Johnson Research Foundation, Department of Biochemistry and Biophysics, University of Pennsylvania, Philadelphia, PA, USA.
authors.forename: He N
authors.initials: HN
authors.lastname: Xu
authors.affiliation: Department of Radiology, University of Pennsylvania, Philadelphia, PA, USA.|Britton Chance Laboratory of Redox Imaging, Johnson Research Foundation, Department of Biochemistry and Biophysics, University of Pennsylvania, Philadelphia, PA, USA.
authors.forename: Tahreem A
authors.initials: TA
authors.lastname: Mir
authors.affiliation: Department of Radiology, University of Pennsylvania, Philadelphia, PA, USA.
authors.forename: Seung-Cheol
authors.initials: SC
authors.lastname: Lee
authors.affiliation: Department of Radiology, University of Pennsylvania, Philadelphia, PA, USA.|Britton Chance Laboratory of Redox Imaging, Johnson Research Foundation, Department of Biochemistry and Biophysics, University of Pennsylvania, Philadelphia, PA, USA.
authors.forename: Min
authors.initials: M
authors.lastname: Feng
authors.affiliation: Department of Radiology, University of Pennsylvania, Philadelphia, PA, USA.|Britton Chance Laboratory of Redox Imaging, Johnson Research Foundation, Department of Biochemistry and Biophysics, University of Pennsylvania, Philadelphia, PA, USA.
authors.forename: Namisa
authors.initials: N
authors.lastname: Farhad
authors.affiliation: Department of Biomedical Engineering, University of Rochester, Rochester, NY, USA.
authors.forename: Regine
authors.initials: R
authors.lastname: Choe
authors.affiliation: Department of Radiology, University of Pennsylvania, Philadelphia, PA, USA.
authors.forename: Jerry D
authors.initials: JD
authors.lastname: Glickson
authors.affiliation: Department of Radiology, University of Pennsylvania, Philadelphia, PA, USA. linli@mail.med.upenn.edu.|Britton Chance Laboratory of Redox Imaging, Johnson Research Foundation, Department of Biochemistry and Biophysics, University of Pennsylvania, Philadelphia, PA, USA. linli@mail.med.upenn.edu.|Abramson Cancer Center, University of Pennsylvania, Philadelphia, PA, USA. linli@mail.med.upenn.edu.|Institute of Translational Medicine and Therapeutics, University of Pennsylvania, Philadelphia, PA, USA. linli@mail.med.upenn.edu.
authors.forename: Lin Z
authors.initials: LZ
authors.lastname: Li
mesh_terms: D000818:Animals; D000971:Antineoplastic Combined Chemotherapy Protocols / Q000008:administration & dosage / Q000494:pharmacology*; D045744:Cell Line, Tumor; D003520:Cyclophosphamide / Q000008:administration & dosage; D004317:Doxorubicin / Q000008:administration & dosage; D005260:Female; D006801:Humans; D016403:Lymphoma, Large B-Cell, Diffuse / Q000188:drug therapy* / Q000378:metabolism*; D008228:Lymphoma, Non-Hodgkin / Q000188:drug therapy* / Q000378:metabolism*; D051379:Mice; D008819:Mice, Nude; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D009243:NAD / Q000378:metabolism; D010084:Oxidation-Reduction; D011241:Prednisone / Q000008:administration & dosage; D014750:Vincristine / Q000008:administration & dosage; D023041:Xenograft Model Antitumor Assays
chemical_list: D009243:NAD; D014750:Vincristine; D004317:Doxorubicin; D003520:Cyclophosphamide; D011241:Prednisone