title: Epigallocatechin gallate effectively ameliorates fluoride-induced oxidative stress and DNA damage in the liver of rats.
abstract: Environmental exposure to sodium fluoride (NaF) compounds is a worldwide health concern. Epigallocatechin gallate (EGCG) is a green tea catechin found in a variety of green tea preparations. The intention of this study was to investigate the hepatoprotective role of EGCG in NaF-intoxicated rats. Rats were orally treated with NaF alone (25 mg·(kg body mass)(-1)·day(-1)) or plus EGCG at different doses (20, 40, and 80 mg·(kg body mass)(-1)·day(-1)) for 4 weeks. Hepatotoxicity of NaF was determined by increased levels of serum hepatospecific markers and total bilirubin, along with increased levels of thiobarbituric acid reactive substances, lipid hydroperoxides, protein carbonyl content, and conjugated dienes. The hepatotoxic nature of NaF was further evidenced by the decreased activity of enzymatic and nonenzymatic antioxidant levels in liver. NaF-treated rats also showed increased DNA damage and fragmentation in hepatocytes. Administration of EGCG (40 mg·(kg body mass)(-1)) to NaF-intoxicated rats significantly recuperated the distorted biochemical indices, DNA damage, and pathological changes in the liver tissue. Thus, the results of the present study clearly demonstrate that EGCG has strong free radical scavenging, antioxidant, and antigenotoxic properties that protect against NaF-induced oxidative hepatic injury in rats.
authors.affiliation: Department of Zoology, Annamalai University, Annamalainagar 608002, Tamilnadu, India.
authors.forename: Shanmugam
authors.initials: S
authors.lastname: Thangapandiyan
authors.forename: Selvaraj
authors.initials: S
authors.lastname: Miltonprabu
mesh_terms: D000251:Adenosine Triphosphatases / Q000378:metabolism; D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D001663:Bilirubin / Q000097:blood / Q000378:metabolism; D015415:Biomarkers / Q000097:blood / Q000378:metabolism; D015992:Body Mass Index; D002392:Catechin / Q000031:analogs & derivatives* / Q000494:pharmacology; D004249:DNA Damage; D004435:Eating / Q000187:drug effects / Q000235:genetics; D005459:Fluorides; D016166:Free Radical Scavengers / Q000494:pharmacology; D022781:Hepatocytes / Q000187:drug effects / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008054:Lipid Peroxides / Q000378:metabolism; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects* / Q000235:genetics; D010865:Pilot Projects; D050050:Protein Carbonylation / Q000187:drug effects / Q000235:genetics; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D016166:Free Radical Scavengers; D008054:Lipid Peroxides; D017392:Thiobarbituric Acid Reactive Substances; D002392:Catechin; C045651:epigallocatechin gallate; D000251:Adenosine Triphosphatases; D005459:Fluorides; D001663:Bilirubin