title: Quantitative in vivo detection of brain cell death after hypoxia ischemia using the lipid peak at 1.3 ppm of proton magnetic resonance spectroscopy in neonatal rats.
abstract: This study was performed to determine the accuracy of proton magnetic spectroscopy ((1)H-MRS) lipid peak as a noninvasive tool for quantitative in vivo detection of brain cell death. Seven day-old Sprague Dawley rats were subjected to 8% oxygen following a unilateral carotid artery ligation. For treatment, cycloheximide was given immediately after hypoxic ischemia (HI). Lipid peak was measured using (1)H-MRS at 24 hr after HI, and then brains were harvested for fluorocytometric analyses with annexin V/propidium iodide (PI) and fluorescent probe JC-1, and for adenosine-5'-triphosphate (ATP) and lactate. Increased lipid peak at 1.3 ppm measured with (1)H-MRS, apoptotic and necrotic cells, and loss of mitochondrial membrane potential (ΔΨ) at 24 hr after HI were significantly improved with cycloheximide treatment. Significantly reduced brain ATP and increased lactate levels observed at 24 hr after HI showed a tendency to improve without statistical significance with cycloheximide treatment. Lipid peak at 1.3 ppm showed significant positive correlation with both apoptotic and necrotic cells and loss of ΔΨ, and negative correlation with normal live cells. Lipid peak at 1.3 ppm measured by (1)H-MRS might be a sensitive and reliable diagnostic tool for quantitative in vivo detection of brain cell death after HI.
authors.affiliation: Department of Pediatrics, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea.
authors.forename: So Yoon
authors.initials: SY
authors.lastname: Ahn
authors.forename: Hye Soo
authors.initials: HS
authors.lastname: Yoo
authors.forename: Jang Hoon
authors.initials: JH
authors.lastname: Lee
authors.forename: Dong Kyung
authors.initials: DK
authors.lastname: Sung
authors.forename: Yu Jin
authors.initials: YJ
authors.lastname: Jung
authors.forename: Se In
authors.initials: SI
authors.lastname: Sung
authors.forename: Keun Ho
authors.initials: KH
authors.lastname: Lim
authors.forename: Yun Sil
authors.initials: YS
authors.lastname: Chang
authors.forename: Jung Hee
authors.initials: JH
authors.lastname: Lee
authors.forename: Ki Soo
authors.initials: KS
authors.lastname: Kim
authors.forename: Won Soon
authors.initials: WS
authors.lastname: Park
mesh_terms: D000255:Adenosine Triphosphate / Q000032:analysis*; D000818:Animals; D000831:Animals, Newborn; D017209:Apoptosis; D001921:Brain / Q000378:metabolism / Q000473:pathology; D003513:Cycloheximide / Q000494:pharmacology; D020925:Hypoxia-Ischemia, Brain / Q000378:metabolism* / Q000473:pathology*; D019344:Lactic Acid / Q000032:analysis*; D008055:Lipids / Q000032:analysis*; D009682:Magnetic Resonance Spectroscopy; D053078:Membrane Potential, Mitochondrial; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D008055:Lipids; D019344:Lactic Acid; D000255:Adenosine Triphosphate; D003513:Cycloheximide
keywords: Animals; Flow Cytometry; Hypoxia-Ischemia, Brain; Magnetic Resonance Spectroscopy; Newborn