title: Intracellular click reaction with a fluorescent chemical Ca2+ indicator to prolong its cytosolic retention.
abstract: The powerful strategy of "intracellular click reaction" was used to retain a chemical Ca(2+) indicator in the cytosol. Specifically, a novel clickable Ca(2+) indicator "N3-fura-2 AM" was coupled with dibenzylcyclooctyl-modified biomacromolecules via copper-free click reaction in living cells and Ca(2+) oscillation was observed for an extended period of time.
authors.affiliation: Department of Life Science & Medical Bioscience, Graduate School of Advanced Science & Engineering, Waseda University, 02C227, TWIns, Tokyo, 162-8480, Japan.
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Takei
authors.forename: Atsushi
authors.initials: A
authors.lastname: Murata
authors.forename: Kento
authors.initials: K
authors.lastname: Yamagishi
authors.forename: Satoshi
authors.initials: S
authors.lastname: Arai
authors.forename: Hideki
authors.initials: H
authors.lastname: Nakamura
authors.forename: Takafumi
authors.initials: T
authors.lastname: Inoue
authors.forename: Shinji
authors.initials: S
authors.lastname: Takeoka
mesh_terms: D002118:Calcium / Q000737:chemistry*; D057930:Click Chemistry; D003600:Cytosol / Q000737:chemistry* / Q000378:metabolism; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry* / Q000493:pharmacokinetics; D006367:HeLa Cells; D006801:Humans; D015394:Molecular Structure
chemical_list: D005456:Fluorescent Dyes; D002118:Calcium