title: Technological advances in site-directed spin labeling of proteins.
abstract: Molecular flexibility over a wide time range is of central importance to the function of many proteins, both soluble and membrane. Revealing the modes of flexibility, their amplitudes, and time scales under physiological conditions is the challenge for spectroscopic methods, one of which is site-directed spin labeling EPR (SDSL-EPR). Here we provide an overview of some recent technological advances in SDSL-EPR related to investigation of structure, structural heterogeneity, and dynamics of proteins. These include new classes of spin labels, advances in measurement of long range distances and distance distributions, methods for identifying backbone and conformational fluctuations, and new strategies for determining the kinetics of protein motion.
authors.affiliation: Jules Stein Eye Institute and Department of Chemistry and Biochemistry, University of California, Los Angeles, CA 90095, United States. Electronic address: HubbellW@jsei.ucla.edu.
authors.forename: Wayne L
authors.initials: WL
authors.lastname: Hubbell
authors.forename: Carlos J
authors.initials: CJ
authors.lastname: López
authors.forename: Christian
authors.initials: C
authors.lastname: Altenbach
authors.forename: Zhongyu
authors.initials: Z
authors.lastname: Yang
mesh_terms: D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D009589:Nitrogen Oxides / Q000737:chemistry; D011487:Protein Conformation; D011506:Proteins / Q000737:chemistry*; D013113:Spin Labels; D013696:Temperature
chemical_list: D009589:Nitrogen Oxides; D011506:Proteins; D013113:Spin Labels; C039900:nitroxyl