title: Naphthyridinomycin biosynthesis revealing the use of leader peptide to guide nonribosomal peptide assembly.
abstract: Analysis of naphthyridinomycin gene cluster revealed that this antibiotic is generated by nonribosomal peptide synthetase (NRPS) machinery. However, four modules encoded by two genes do not correspond with the structural units in the final product. Genetic and biochemical characterization of the gene cluster suggested that the leader peptide mechanism for the NRPS assembly line was involved in biosynthesis of this tetrahydroisoquinoline alkaloid.
authors.affiliation: State Key Laboratory of Bioorganic and Natural Products Chemistry, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, 345 Lingling Road, Shanghai, 200032, China.
authors.forename: Jin-Yue
authors.initials: JY
authors.lastname: Pu
authors.forename: Chao
authors.initials: C
authors.lastname: Peng
authors.forename: Man-Cheng
authors.initials: MC
authors.lastname: Tang
authors.forename: Yue
authors.initials: Y
authors.lastname: Zhang
authors.forename: Jian-Ping
authors.initials: JP
authors.lastname: Guo
authors.forename: Li-Qiang
authors.initials: LQ
authors.lastname: Song
authors.forename: Qiang
authors.initials: Q
authors.lastname: Hua
authors.forename: Gong-Li
authors.initials: GL
authors.lastname: Tang
mesh_terms: D000470:Alkaloids / Q000737:chemistry* / Q000378:metabolism*; D000595:Amino Acid Sequence; D015394:Molecular Structure; D005810:Multigene Family; D009287:Naphthyridines / Q000737:chemistry / Q000378:metabolism; D010453:Peptide Synthases / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism*; D010455:Peptides / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism*; D044005:Tetrahydroisoquinolines / Q000737:chemistry* / Q000378:metabolism*
chemical_list: D000470:Alkaloids; D009287:Naphthyridines; D010455:Peptides; D044005:Tetrahydroisoquinolines; C010752:naphthyridinomycin; D010453:Peptide Synthases; C104385:non-ribosomal peptide synthase