title: [Construction of RNAi vector of dopamine D1 receptor and identification of its silencing effects].
abstract: OBJECTIVE
  To construct dopamine D1 receptor (DRD1) expression interference vectors to study the role of DRD1 in nerve cells and lay a foundation for drug development in anti-convulsion.
  
  
  METHODS
  Based on DRD1 gene sequence in GenBank, 10 interfere vectors of DRD1 were designed. Liposomal was used to transfect NG-108-15 and the transfect effect was assayed by GFP. With realtime PCR and Western blot, the DRD1 expression was detected.
  
  
  RESULTS
  The 10 constructed interfere vectors transfected into NG-108-15 cells by liposomal method and inhibited DRD1 mRNA and protein expression. DRD1 mRNA expression in NG-108-15 cells transfected with pGPU6-GFP-Neo-si-DRD1-5 was the lowest whereas DRD1 protein expression in NG-108-15 cells transfected with pGPU6-GFP-Neo-si-DRD1-1, -2, -6, -7 was the lowest.
  
  
  CONCLUSIONS
  DRD1 expression interference vector is successfully constructed.
authors.affiliation: Department of Anesthesiology, Central South University, Changsha, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Li
authors.forename: Junmei
authors.initials: J
authors.lastname: Xu
authors.forename: Guixiu
authors.initials: G
authors.lastname: Yuan
authors.forename: Jin
authors.initials: J
authors.lastname: Li
authors.forename: Zhangling
authors.initials: Z
authors.lastname: Chen
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D005822:Genetic Vectors; D005910:Glioma / Q000473:pathology; D006822:Hybrid Cells; D008081:Liposomes / Q000378:metabolism; D051379:Mice; D009447:Neuroblastoma / Q000473:pathology; D034622:RNA Interference; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D034741:RNA, Small Interfering / Q000235:genetics*; D017447:Receptors, Dopamine D1 / Q000235:genetics* / Q000378:metabolism; D014162:Transfection
chemical_list: D008081:Liposomes; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D017447:Receptors, Dopamine D1