title: HPV frequency in penile carcinoma of Mexican patients: important contribution of HPV16 European variant.
abstract: The role of human papillomavirus (HPV) infection in penile carcinoma (PeC) is currently reported and about half of the PeC is associated with HPV16 and 18. We used a PCR-based strategy by using HPV general primers to analyze 86 penile carcinomas paraffin-embedded tissues. Some clinical data, the histological subtype, growth pattern, and differentiation degree were also collected. The amplified fragments were then sequenced to confirm the HPV type and for HPV16/18 variants. DNA samples were also subjected to relative real time PCR for hTERC gene copy number. Some clinical data were also collected. Global HPV frequency was 77.9%. Relative contributions was for HPV16 (85%), 31 (4.4%), 11 (4.4%), 58, 33, 18, and 59 (1.4% each one). Sequence analysis of HPV16 identified European variants and Asian-American (AAb-c) variants in 92% and in 8% of the samples, respectively. Furthermore hTERC gene amplification was observed in only 17% of the cases. Our results suggest that some members of HPV A9 group (represented by HPV16, 58, and 31) are the most frequent among PeC patients studied with an important contribution from HPV16 European variant. The hTERC gene amplification could be poorly related to penile epithelial tissue.
authors.affiliation: Laboratorio de Oncología Genómica, Unidad de Investigación Médica en Enfermedades Oncológicas, Hospital de Oncología, Centro Médico Nacional Siglo XXI, Instituto Mexicano del Seguro Social, México DF.
authors.forename: Ricardo
authors.initials: R
authors.lastname: López-Romero
authors.forename: Candela
authors.initials: C
authors.lastname: Iglesias-Chiesa
authors.forename: Brenda
authors.initials: B
authors.lastname: Alatorre
authors.forename: Karla
authors.initials: K
authors.lastname: Vázquez
authors.forename: Patricia
authors.initials: P
authors.lastname: Piña-Sánchez
authors.forename: Isabel
authors.initials: I
authors.lastname: Alvarado
authors.forename: Minerva
authors.initials: M
authors.lastname: Lazos
authors.forename: Raúl
authors.initials: R
authors.lastname: Peralta
authors.forename: Beatriz
authors.initials: B
authors.lastname: González-Yebra
authors.forename: Anae
authors.initials: A
authors.lastname: Romero
authors.forename: Mauricio
authors.initials: M
authors.lastname: Salcedo
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000235:genetics; D001706:Biopsy; D002277:Carcinoma / Q000453:epidemiology / Q000235:genetics / Q000473:pathology / Q000821:virology*; D016009:Chi-Square Distribution; D004279:DNA, Viral / Q000302:isolation & purification; D005784:Gene Amplification; D005838:Genotype; D061809:Human Papillomavirus DNA Tests; D052162:Human papillomavirus 16 / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D008297:Male; D008800:Mexico / Q000453:epidemiology; D008875:Middle Aged; D030361:Papillomavirus Infections / Q000453:epidemiology / Q000821:virology*; D016612:Paraffin Embedding; D010412:Penile Neoplasms / Q000453:epidemiology / Q000235:genetics / Q000473:pathology / Q000821:virology*; D010641:Phenotype; D011237:Predictive Value of Tests; D012313:RNA / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D012307:Risk Factors; D019098:Telomerase / Q000235:genetics; D055815:Young Adult
chemical_list: D014408:Biomarkers, Tumor; D004279:DNA, Viral; C422952:telomerase RNA; D012313:RNA; D019098:Telomerase
keywords: HPV; México; penile carcinoma