title: Ascomycota members dominate fungal communities during straw residue decomposition in arable soil.
abstract: This study investigated the development of fungal community composition in arable soil during the degradation of straw residue. We explored the short-term responses of the fungal community over 28 days of decomposition in soil using culture-independent polymerase chain reaction in combination with a clone library and denaturing gradient gel electrophoresis (DGGE). Fungal cellobiohydrolase I (cbhI) genes in the soil were also characterized, and their diversity suggested the existence of a different cellulose decomposer. The DGGE profiles based on fungal internal transcribed spacer analysis showed different successions of fungal populations during residue decomposition. Members of Lecythophora and Sordariales were dominant in the early succession, while Hypocrea and Engyodontium were better adapted in the late succession. The succession of fungal communities might be related to changes of residue quality during decomposition. Collectively, sequences assigned to Ascomycota members were dominant at different stages of the fungal succession during decomposition, revealing that they were key drivers responsible for residue degradation in the arable soil tested.
authors.affiliation: Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, PR China.
authors.forename: Anzhou
authors.initials: A
authors.lastname: Ma
authors.forename: Xuliang
authors.initials: X
authors.lastname: Zhuang
authors.forename: Junmei
authors.initials: J
authors.lastname: Wu
authors.forename: Mengmeng
authors.initials: M
authors.lastname: Cui
authors.forename: Di
authors.initials: D
authors.lastname: Lv
authors.forename: Chunzhao
authors.initials: C
authors.lastname: Liu
authors.forename: Guoqiang
authors.initials: G
authors.lastname: Zhuang
mesh_terms: D001203:Ascomycota / Q000201:enzymology / Q000235:genetics*; D044822:Biodiversity; D043366:Cellulose 1,4-beta-Cellobiosidase / Q000235:genetics; D004271:DNA, Fungal / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D058645:Denaturing Gradient Gel Electrophoresis; D005656:Fungal Proteins / Q000235:genetics; D005819:Genetic Markers; D008969:Molecular Sequence Data; D058889:Molecular Typing; D016533:Mycological Typing Techniques; D012275:Oryza / Q000382:microbiology*; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology
chemical_list: D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer; D005656:Fungal Proteins; D005819:Genetic Markers; D043366:Cellulose 1,4-beta-Cellobiosidase