title: Sericin-carboxymethyl cellulose porous matrices as cellular wound dressing material.
abstract: In this study, porous three-dimensional (3D) hydrogel matrices are fabricated composed of silk cocoon protein sericin of non-mulberry silkworm Antheraea mylitta and carboxymethyl cellulose. The matrices are prepared via freeze-drying technique followed by dual cross-linking with glutaraldehyde and aluminum chloride. The microstructure of the hydrogel matrices is assessed using scanning electron microscopy and biophysical characterization are carried out using Fourier transform infrared spectroscopy and X-ray diffraction. The transforming growth factor β1 release from the cross-linked matrices as a growth factor is evaluated by immunosorbent assay. Live dead assay and 3-[4,5-dimethylthiazolyl-2]-2,5-diphenyl tetrazolium bromide assay show no cytotoxicity of blended matrices toward human keratinocytes. The matrices support the cell attachment and proliferation of human keratinocytes as observed through scanning electron microscope and confocal images. Gelatin zymography demonstrates the low levels of matrix metalloproteinase 2 (MMP-2) and insignificant amount of MMP-9 in the culture media of cell seeded matrices. Low inflammatory response of the matrices is indicated through tumor necrosis factor alpha release assay. The results indicate that the fabricated matrices constitute 3D cell-interactive environment for tissue engineering applications and its potential use as a future cellular biological wound dressing material.
authors.affiliation: Department of Biotechnology, Indian Institute of Technology, Kharagpur, 7213 02, West Bengal, India.
authors.forename: Sunita
authors.initials: S
authors.lastname: Nayak
authors.forename: S C
authors.initials: SC
authors.lastname: Kundu
mesh_terms: D000818:Animals; D001458:Bandages; D001672:Biocompatible Materials / Q000737:chemistry*; D002266:Carboxymethylcellulose Sodium / Q000737:chemistry*; D002448:Cell Adhesion / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D003692:Delayed-Action Preparations / Q000737:chemistry; D006801:Humans; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry; D015603:Keratinocytes / Q000166:cytology / Q000187:drug effects; D009036:Moths / Q000737:chemistry*; D016062:Porosity; D047030:Sericins / Q000737:chemistry*; D053773:Transforming Growth Factor beta1 / Q000008:administration & dosage / Q000494:pharmacology; D014945:Wound Healing
chemical_list: D001672:Biocompatible Materials; D003692:Delayed-Action Preparations; D047030:Sericins; D053773:Transforming Growth Factor beta1; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D002266:Carboxymethylcellulose Sodium
keywords: Antheraea mylitta; growth factor; hydrogel; sericin; wound dressing