title: Enhancing nitrilase production from Fusarium proliferatum using response surface methodology.
abstract: The individual and interactive effects of three independent variables i.e. carbon source (glucose), nitrogen source (sodium nitrate) and inducer (ϵ-caprolactam) on nitrilase production from Fusarium proliferatum were investigated using design of experiments (DOE) methodology. Response surface methodology (RSM) was followed to generate the process model and to obtain the optimal conditions for maximum nitrilase production. Based on central composite design (CCD) a quadratic model was found to fit the experimental data (p<0.0001) and maximum activity of 59.0U/g biomass was predicted at glucose concentration (53.22 g/l), sodium nitrate (2.31 g/l) and ϵ-caprolactam (3.58 g/l). Validation experiments were carried out under the optimized conditions for verification of the model. The nitrilase activity of 58.3U/g biomass obtained experimentally correlated to the predicted activity which proves the authenticity of the model. Overall 2.24 fold increase in nitrilase activity was achieved as compared to the activity before optimization (26U/g biomass).
authors.affiliation: Fermentation Technology Division, Indian Institute of Integrative Medicine, Canal Road, Jammu Tawi, 180001 India.
authors.forename: Farnaz
authors.initials: F
authors.lastname: Yusuf
authors.forename: Asha
authors.initials: A
authors.lastname: Chaubey
authors.forename: Arvind
authors.initials: A
authors.lastname: Raina
authors.forename: Urmila
authors.initials: U
authors.lastname: Jamwal
authors.forename: Rajinder
authors.initials: R
authors.lastname: Parshad
keywords: Central composite design; Design of experiments; Fusarium proliferatum; Nitrilase; Response surface methodology