title: A TILLING Platform for Functional Genomics in Brachypodium distachyon.
abstract: The new model plant for temperate grasses, Brachypodium distachyon offers great potential as a tool for functional genomics. We have established a sodium azide-induced mutant collection and a TILLING platform, called "BRACHYTIL", for the inbred line Bd21-3. The TILLING collection consists of DNA isolated from 5530 different families. Phenotypes were reported and organized in a phenotypic tree that is freely available online. The tilling platform was validated by the isolation of mutants for seven genes belonging to multigene families of the lignin biosynthesis pathway. In particular, a large allelic series for BdCOMT6, a caffeic acid O-methyl transferase was identified. Some mutants show lower lignin content when compared to wild-type plants as well as a typical decrease of syringyl units, a hallmark of COMT-deficient plants. The mutation rate was estimated at one mutation per 396 kb, or an average of 680 mutations per line. The collection was also used to assess the Genetically Effective Cell Number that was shown to be at least equal to 4 cells in Brachypodium distachyon. The mutant population and the TILLING platform should greatly facilitate functional genomics approaches in this model organism.
authors.affiliation: URGV, Unité de Recherche en Génomique Végétale, Université d'Evry Val d'Essonne, INRA, Evry, France.
authors.forename: Marion
authors.initials: M
authors.lastname: Dalmais
authors.forename: Sébastien
authors.initials: S
authors.lastname: Antelme
authors.forename: Séverine
authors.initials: S
authors.lastname: Ho-Yue-Kuang
authors.forename: Yin
authors.initials: Y
authors.lastname: Wang
authors.forename: Olivier
authors.initials: O
authors.lastname: Darracq
authors.forename: Madeleine Bouvier
authors.initials: MB
authors.lastname: d'Yvoire
authors.forename: Laurent
authors.initials: L
authors.lastname: Cézard
authors.forename: Frédéric
authors.initials: F
authors.lastname: Légée
authors.forename: Eddy
authors.initials: E
authors.lastname: Blondet
authors.forename: Nicolas
authors.initials: N
authors.lastname: Oria
authors.forename: Christelle
authors.initials: C
authors.lastname: Troadec
authors.forename: Véronique
authors.initials: V
authors.lastname: Brunaud
authors.forename: Lise
authors.initials: L
authors.lastname: Jouanin
authors.forename: Herman
authors.initials: H
authors.lastname: Höfte
authors.forename: Abdelafid
authors.initials: A
authors.lastname: Bendahmane
authors.forename: Catherine
authors.initials: C
authors.lastname: Lapierre
authors.forename: Richard
authors.initials: R
authors.lastname: Sibout
mesh_terms: D053898:Biosynthetic Pathways; D058431:Brachypodium / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D018745:Genome, Plant; D023281:Genomics / Q000379:methods*; D008031:Lignin / Q000378:metabolism; D008958:Models, Molecular; D009154:Mutation; D010641:Phenotype; D010802:Phylogeny; D000069600:Plant Breeding; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics*; D017422:Sequence Analysis, DNA
chemical_list: D010940:Plant Proteins; D008031:Lignin