title: Identification of immunity related genes to study the Physalis peruviana--Fusarium oxysporum pathosystem.
abstract: The Cape gooseberry (Physalisperuviana L) is an Andean exotic fruit with high nutritional value and appealing medicinal properties. However, its cultivation faces important phytosanitary problems mainly due to pathogens like Fusarium oxysporum, Cercosporaphysalidis and Alternaria spp. Here we used the Cape gooseberry foliar transcriptome to search for proteins that encode conserved domains related to plant immunity including: NBS (Nucleotide Binding Site), CC (Coiled-Coil), TIR (Toll/Interleukin-1 Receptor). We identified 74 immunity related gene candidates in P. peruviana which have the typical resistance gene (R-gene) architecture, 17 Receptor like kinase (RLKs) candidates related to PAMP-Triggered Immunity (PTI), eight (TIR-NBS-LRR, or TNL) and nine (CC-NBS-LRR, or CNL) candidates related to Effector-Triggered Immunity (ETI) genes among others. These candidate genes were categorized by molecular function (98%), biological process (85%) and cellular component (79%) using gene ontology. Some of the most interesting predicted roles were those associated with binding and transferase activity. We designed 94 primers pairs from the 74 immunity-related genes (IRGs) to amplify the corresponding genomic regions on six genotypes that included resistant and susceptible materials. From these, we selected 17 single band amplicons and sequenced them in 14 F. oxysporum resistant and susceptible genotypes. Sequence polymorphisms were analyzed through preliminary candidate gene association, which allowed the detection of one SNP at the PpIRG-63 marker revealing a nonsynonymous mutation in the predicted LRR domain suggesting functional roles for resistance.
authors.affiliation: Plant Molecular Genetics Laboratory, Center for Biotechnology and Bioindustry, Colombian Corporation for Agricultural Research, CORPOICA, Bogotá, Colombia.
authors.forename: Felix E
authors.initials: FE
authors.lastname: Enciso-Rodríguez
authors.forename: Carolina
authors.initials: C
authors.lastname: González
authors.forename: Edwin A
authors.initials: EA
authors.lastname: Rodríguez
authors.forename: Camilo E
authors.initials: CE
authors.lastname: López
authors.forename: David
authors.initials: D
authors.lastname: Landsman
authors.forename: Luz Stella
authors.initials: LS
authors.lastname: Barrero
authors.forename: Leonardo
authors.initials: L
authors.lastname: Mariño-Ramírez
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D030541:Databases, Genetic; D060467:Disease Resistance / Q000235:genetics / Q000276:immunology; D060585:Fusariosis / Q000235:genetics* / Q000276:immunology*; D005670:Fusarium / Q000276:immunology*; D054884:Host-Pathogen Interactions / Q000235:genetics / Q000276:immunology; D058977:Molecular Sequence Annotation; D010641:Phenotype; D032321:Physalis / Q000235:genetics* / Q000276:immunology* / Q000382:microbiology; D010935:Plant Diseases / Q000235:genetics* / Q000276:immunology*; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D020641:Polymorphism, Single Nucleotide; D059467:Transcriptome
chemical_list: D010940:Plant Proteins