title: Speckle-based configuration for simultaneous in vitro inspection of mechanical contractions of cardiac myocyte cells.
abstract: An optical lensless configuration for a remote noncontact measuring of mechanical contractions of a vast number of cardiac myocytes is proposed. All the myocytes were taken from rats, and the measurements were done in an in vitro mode. The optical method is based on temporal analysis of secondary reflected speckle patterns generated in lensless microscope configuration. The processing involves analyzing the movement and the change in the statistics of the secondary speckle patterns that are created on top of the cell culture when it is illuminated by a spot of laser beam. The main advantage of the proposed system is the ability to measure many cells simultaneously (∼1000 cells) and to extract the statistical data of their movement at once. The presented experimental results also include investigation of the effect of isoproteranol on cell contraction process.
authors.affiliation: Tel Aviv University, Department of Biomedical Engineering, Tel-Aviv 69978, Israel.
authors.forename: Mark
authors.initials: M
authors.lastname: Golberg
authors.forename: Dror
authors.initials: D
authors.lastname: Fixler
authors.forename: Asher
authors.initials: A
authors.lastname: Shainberg
authors.forename: Sharon
authors.initials: S
authors.lastname: Zlochiver
authors.forename: Vicente
authors.initials: V
authors.lastname: Micó
authors.forename: Javier
authors.initials: J
authors.lastname: Garcia
authors.forename: Yevgeny
authors.initials: Y
authors.lastname: Beiderman
authors.forename: Zeev
authors.initials: Z
authors.lastname: Zalevsky
mesh_terms: D000818:Animals; D001696:Biomechanical Phenomena; D002478:Cells, Cultured; D004558:Electric Stimulation; D007545:Isoproterenol / Q000494:pharmacology; D008853:Microscopy / Q000379:methods*; D009200:Myocardial Contraction; D032383:Myocytes, Cardiac / Q000166:cytology* / Q000187:drug effects; D061848:Optical Imaging / Q000379:methods*; D051381:Rats; D015203:Reproducibility of Results; D012815:Signal Processing, Computer-Assisted
chemical_list: D007545:Isoproterenol