title: Mitigation of peroxidative stress for barley exposed to cadmium in the presence of water-extractable organic matter from compost-like materials.
abstract: The effects of water-extractable organic matter (WEOM) from compost-like materials on peroxidative stress were investigated for hydroponic culture of barley exposed to Cd. In the presence of WEOM, lipoxygenase activity and malondialdehyde, indices of peroxidative stress in barley, were significantly reduced, compared to those with Cd alone (5 μM) for a 30-d culture (p<0.05). In addition, Cd uptake in the presence of WEOM samples was significantly lower than that in their absence (p<0.05). These results indicate that the addition of WEOM can be effective in mitigating the peroxidative stress in barley exposed to Cd. Of the total Cd in the solution, 7-8% was complexed with WEOM, indicating that the complexation of Cd with WEOM is a minor factor in reducing Cd-induced stress in barley. The WEOM sample was purified by cation-exchange column and ultrafiltration to remove the nutrient minerals, such as Ca, Mg and Fe. When the purified WEOM was employed for hydroponic culture in the presence of Cd, significant decreases in peroxidative stress and Cd uptake were observed (p<0.05). These results show that the organic components in WEOM contribute to the mitigation of peroxidative stress in barley exposed to Cd.
authors.affiliation: Laboratory of Chemical Resources, Division of Sustainable Resources Engineering, Graduate School of Engineering, Hokkaido University, Sapporo 060-8628, Japan.
authors.forename: Naoya
authors.initials: N
authors.lastname: Tachibana
authors.forename: Kenya
authors.initials: K
authors.lastname: Nagasawa
authors.forename: Binhui
authors.initials: B
authors.lastname: Wang
authors.forename: Kazuki
authors.initials: K
authors.lastname: Nishiya
authors.forename: Masami
authors.initials: M
authors.lastname: Fukushima
authors.forename: Hikari
authors.initials: H
authors.lastname: Kanno
authors.forename: Takuro
authors.initials: T
authors.lastname: Shinano
authors.forename: Keiki
authors.initials: K
authors.lastname: Okazaki
mesh_terms: D002104:Cadmium / Q000032:analysis / Q000633:toxicity*; D001467:Hordeum / Q000502:physiology*; D012987:Soil / Q000737:chemistry*; D012989:Soil Pollutants / Q000032:analysis / Q000633:toxicity*; D013312:Stress, Physiological / Q000502:physiology*; D014865:Waste Disposal, Fluid
chemical_list: D012987:Soil; D012989:Soil Pollutants; D002104:Cadmium
keywords: Barley; Cadmium; Compost-like materials; Lipoxygenase; Malondialdehyde; Water-extractable organic matter