title: Algimonas ampicilliniresistens sp. nov., isolated from the red alga Porphyra yezoensis, and emended description of the genus Algimonas.
abstract: Three strains (14A-2-7(T), 14A-3-1 and 14A-3) of Gram-stain-negative, prosthecate, motile bacteria were isolated from an algal medium supplemented with 10 mg ampicillin l(-1) (w/v), in which the red alga Porphyra yezoensis had been cultured. Based on the 16S rRNA gene sequence analysis, the three isolates formed a cluster with the genus Algimonas of the family Hyphomonadaceae. The sequences of the three isolates had high similarity with those of Algimonas porphyrae 0C-2-2(T) (97.6 % similarity) and Litorimonas taeanensis G5(T) (95.6 % similarity). The DNA G+C contents of the three isolates ranged from 54.3 to 55.0 mol%, which were more similar to that of A. porphyrae 0C-2-2(T) (58.5 mol%) than to that of L. taeanensis G5(T) (47.1 mol%). The DNA-DNA relatedness showed that the three isolates were representatives of the same species (88.1-94.0 % relatedness) and that strain 14A-2-7(T) was a representative of a different species from A. porphyrae 0C-2-2(T) and L. taeanensis G5(T) (1.2-8.6 % relatedness). The phenotypic characteristics of strain 14A-2-7(T) differed by 20 results and 30 results from A. porphyrae 0C-2-2(T) and L. taeanensis G5(T), respectively. The three isolates contained ubiquinone-10 as the predominant quinone and C18 : 1ω7c as the major fatty acid. Based on the polyphasic taxonomic analysis, the three isolates represent a novel species of the genus Algimonas, for which the name Algimonas ampicilliniresistens sp. nov. is proposed. The type strain is 14A-2-7(T) ( = LMG 26421(T) = NBRC 108219(T)). An emended description of the genus Algimonas is also proposed.
authors.affiliation: National Research Institute of Fisheries Science, Fisheries Research Agency, Yokohama 236-8648, Japan.
authors.forename: Youhei
authors.initials: Y
authors.lastname: Fukui
authors.affiliation: Seikai National Fisheries Research Institute, Fisheries Research Agency, Nagasaki 851-2213, Japan.
authors.forename: Masahiro
authors.initials: M
authors.lastname: Kobayashi
authors.affiliation: National Research Institute of Fisheries Science, Fisheries Research Agency, Yokohama 236-8648, Japan.
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Saito
authors.affiliation: National Research Institute of Fisheries and Environment of Inland Sea, Fisheries Research Agency, Hatsukaichi 739-0452, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Oikawa
authors.affiliation: Hokkaido National Fisheries Research Institute, Fisheries Research Agency, Sapporo 062-0922, Japan.
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Yano
authors.affiliation: National Research Institute of Fisheries Science, Fisheries Research Agency, Yokohama 236-8648, Japan.
authors.forename: Masataka
authors.initials: M
authors.lastname: Satomi
mesh_terms: D020561:Alphaproteobacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D044664:Porphyra / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D014451:Ubiquinone / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; C025204:Ubiquinone Q2