title: Maternal antibodies or nonproductive infections confound the need for rederivation.
abstract: After rederivation of a mouse parvovirus (MPV)-contaminated transgenic mouse strain, serology and PCR testing of the surrogate dam showed it to be infected with mouse parvovirus strain 1 (MPV-1). The rederived pups (n = 3) also were MPV-positive, according to serology. Despite MPV seropositivity, fecal PCR tests of the pups were negative, as were serologic results from direct-contact sentinels. Only one rederived pup survived, and this male was bred successfully. None of its mates or progeny seroconverted to MPV. At 14.5 mo of age, the rederived male mouse was euthanized; tissues were collected and submitted for MPV testing; both serologic tests and PCR analysis of mesenteric lymph nodes were MPV-negative. One explanation for the rederived pups' MPV seropostivity is passive transfer of maternal antibodies or a nonproductive MPV infection. This case illustrates that although routine serological testing of surrogate mothers and pups is appropriate, any positive results should be further investigated by using transmissibility testing (fecal PCR or contact sentinels or both) prior to repeat rederivation.
authors.affiliation: Department of Comparative Medicine, Stanford University School of Medicine, Stanford, CA, USA. cnagamin@stanford.edu
authors.forename: Claude M
authors.initials: CM
authors.lastname: Nagamine
authors.forename: Lei
authors.initials: L
authors.lastname: Chen
authors.forename: Wen Qi
authors.initials: WQ
authors.lastname: Ho
authors.forename: Stephen A
authors.initials: SA
authors.lastname: Felt
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D005243:Feces / Q000821:virology; D005260:Female; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D010322:Parvoviridae Infections / Q000635:transmission / Q000662:veterinary* / Q000821:virology; D017992:Parvovirus / Q000235:genetics; D016133:Polymerase Chain Reaction; D012376:Rodent Diseases / Q000635:transmission* / Q000821:virology; D017201:Virus Shedding
chemical_list: D000914:Antibodies, Viral