title: BCL10 expression and localization in ocular adnexa MALT lymphomas: a comparative cytogenetic and immunohistochemical study.
abstract: T(1;14) (p22;q32) involving BCL10 and IGH genes is a rare but recurrent chromosomal aberration in MALT-type lymphoma. It is rarely described in ocular adnexa B cell lymphomas, although nuclear BCL10 shuttling seems to be critical for disease progression in this district. We have evaluated the translocations MALT lymphoma-related in a series of 45 ocular adnexa cases, focusing in particular on their relation with BCL10 expression and its cellular topographic distribution. A prognostic tissue microarray (TMA) with ocular adnexa MALT lymphomas was designed. A study of BCL10 expression and its topographic distribution was performed through immunohistochemistry. In addition the assessment of t(14;18) (q32;q21), t(1;14) (p22;q32) and t(11;18) (q21;q21) was determined by Fluorescent In Situ Hybridization (FISH). Our series revealed t(14;18) (q32;q21) in 6/43 cases (14,3%). t(1;14) (p22;q32), never described in ocular adnexa MALT lymphomas, was observed in 3/31 (9,7%), two of which exhibited the gain of 3' upstream BCL10 gene signal (4%), whereas no case showed t(11;18) (q21;q21). Moreover, BCL10 expression was observed in 18/45 cases. In particular its nuclear expression was revealed in 12/45 cases, cytoplasmic expression in 5/45 and both cytoplasmic and nuclear expression in 1/45. Statistical analysis demonstrated that while BCL10 cytoplasmic expression is significantly related to the presence of the investigated chromosomal aberrations, in particular with t(14;18) (q32;q21), BCL10 nuclear shuttling does not show any correlation with these translocations. Our data support that BCL10 nuclear distribution is neither related to BCL10 rearrangement nor to other known translocations.
authors.affiliation: Pathology Unit, "Istituto Nazionale Tumori Fondazione Giovanni Pascale"-IRCCS, Naples, Italy.
authors.forename: Margherita
authors.initials: M
authors.lastname: Cerrone
authors.affiliation: Pathology Unit, "Istituto Nazionale Tumori Fondazione Giovanni Pascale"-IRCCS, Naples, Italy. francesca.collina@virgilio.it.
authors.forename: Francesca
authors.initials: F
authors.lastname: Collina
authors.affiliation: Pathology Unit, "Istituto Nazionale Tumori Fondazione Giovanni Pascale"-IRCCS, Naples, Italy. annadechiara@hotmail.com.
authors.forename: Anna
authors.initials: A
authors.lastname: De Chiara
authors.affiliation: SC. Hematology, "Istituto Nazionale Tumori Fondazione Giovanni Pascale"-IRCCS, Naples, Italy. g.corazzelli@istitutotumori.na.it.
authors.forename: Gaetano
authors.initials: G
authors.lastname: Corazzelli
authors.affiliation: Pathology Unit, "Istituto Nazionale Tumori Fondazione Giovanni Pascale"-IRCCS, Naples, Italy.
authors.forename: Maria Pia
authors.initials: MP
authors.lastname: Curcio
authors.affiliation: Hematology Institute, "Federico II" University, Naples, Italy. aderenzo@unina.it.
authors.forename: Amalia
authors.initials: A
authors.lastname: De Renzo
authors.affiliation: SC. Hematology, "Istituto Nazionale Tumori Fondazione Giovanni Pascale"-IRCCS, Naples, Italy. f.russo@istitutotumori.na.it.
authors.forename: Filippo
authors.initials: F
authors.lastname: Russo
authors.affiliation: Pathology Unit, "Istituto Nazionale Tumori Fondazione Giovanni Pascale"-IRCCS, Naples, Italy. monicantile@libero.it.
authors.forename: Monica
authors.initials: M
authors.lastname: Cantile
authors.affiliation: Biomorphological and Functional Sciences Department, "Federico II" University, Naples, Italy. staibano@unina.it.
authors.forename: Stefania
authors.initials: S
authors.lastname: Staibano
authors.affiliation: Ophthalmology Department, "Federico II" University, Naples, Italy. strianes@unina.it.
authors.forename: Diego
authors.initials: D
authors.lastname: Strianese
authors.affiliation: Ophthalmology Department, "Federico II" University, Naples, Italy. fausto.tranfa@unina.it.
authors.forename: Fausto
authors.initials: F
authors.lastname: Tranfa
authors.affiliation: Pathology Unit, "Istituto Nazionale Tumori Fondazione Giovanni Pascale"-IRCCS, Naples, Italy. gbotti1@alice.it.
authors.forename: Gerardo
authors.initials: G
authors.lastname: Botti
authors.affiliation: Biomorphological and Functional Sciences Department, "Federico II" University, Naples, Italy. gaderosa@unina.it.
authors.forename: Gaetano
authors.initials: G
authors.lastname: De Rosa
authors.affiliation: Pathology Unit, "Istituto Nazionale Tumori Fondazione Giovanni Pascale"-IRCCS, Naples, Italy. r.franco@istitutotumori.na.it.
authors.forename: Renato
authors.initials: R
authors.lastname: Franco
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000096:biosynthesis; D000328:Adult; D000368:Aged; D000074018:B-Cell CLL-Lymphoma 10 Protein; D014408:Biomarkers, Tumor / Q000032:analysis; D020732:Cytogenetic Analysis; D005134:Eye Neoplasms / Q000235:genetics* / Q000378:metabolism* / Q000473:pathology; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D017404:In Situ Hybridization, Fluorescence; D018442:Lymphoma, B-Cell, Marginal Zone / Q000235:genetics* / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D046888:Tissue Array Analysis; D014178:Translocation, Genetic
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D000074018:B-Cell CLL-Lymphoma 10 Protein; C118591:BCL10 protein, human; D014408:Biomarkers, Tumor