title: Effects of microencapsulated Enterococcus fecalis CG1.0007 on growth performance, antioxidation activity, and intestinal microbiota in broiler chickens.
abstract: We performed a series of trials to assess the effect of dietary supplementation with microencapsulated Enterococcus fecalis CG1.0007 on growth performance, antioxidation activity, and intestinal microbiota in Arbor Acres broiler chickens ("broilers"). A total of 150 1-d-old broilers were assigned randomly to 5 feeding treatments (a control group fed the basal diet, 3 groups fed the basal diet plus various concentrations of microencapsulated CG1.0007, and 1 group fed the basal diet plus an antibiotic). Changes in important genera of intestinal bacteria were studied using 16S rRNA gene-based PCR-denaturing gradient gel electrophoresis (DGGE) profiling and real-time quantitative PCR analysis of fecal samples. During the course of the 42-d experimental period, ADG of the birds fed the high and intermediate concentrations of microcapsules were significantly greater (9.90 and 9.50%, respectively) and the ratios of feed to gain fed were significantly lower (4.40 and 4.00%, respectively) compared with the control group. The total antioxidant capacity and the content of malondialdehyde and superoxide dismutase in the microcapsule-treated groups showed significant changes in terms of antioxidation. The numbers of Lactobacillus and Bifidobacterium were significantly greater in the microcapsule-treated groups than in the control group. Cluster analysis indicated that the DGGE bacterial profiles were related to the feeding treatments and revealing the diversity and richness of the intestinal microbiota associated with supplementation of microcapsules. In summary, our results indicate that dietary addition of microencapsulated E. fecalis CG1.0007 enhanced the growth performance of the broilers and improved their health.
authors.affiliation: Academy of State Administration of Grain, Beijing, 100037, PR China.
authors.forename: W
authors.initials: W
authors.lastname: Han
authors.forename: X L
authors.initials: XL
authors.lastname: Zhang
authors.forename: D W
authors.initials: DW
authors.lastname: Wang
authors.forename: L Y
authors.initials: LY
authors.lastname: Li
authors.forename: G L
authors.initials: GL
authors.lastname: Liu
authors.forename: A K
authors.initials: AK
authors.lastname: Li
authors.forename: Y X
authors.initials: YX
authors.lastname: Zhao
mesh_terms: D000821:Animal Feed / Q000032:analysis; D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D001644:Bifidobacterium / Q000187:drug effects / Q000254:growth & development; D002645:Chickens / Q000254:growth & development / Q000382:microbiology* / Q000502:physiology*; D058645:Denaturing Gradient Gel Electrophoresis; D004032:Diet / Q000662:veterinary; D019587:Dietary Supplements / Q000032:analysis; D013293:Enterococcus faecalis / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D005243:Feces / Q000382:microbiology; D005260:Female; D007422:Intestines / Q000382:microbiology*; D007778:Lactobacillus / Q000187:drug effects / Q000254:growth & development; D008297:Male; D064307:Microbiota / Q000187:drug effects; D019936:Probiotics / Q000008:administration & dosage / Q000494:pharmacology*; D012329:RNA, Bacterial / Q000235:genetics / Q000378:metabolism; D012336:RNA, Ribosomal, 16S / Q000235:genetics / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary
chemical_list: D000975:Antioxidants; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S