title: Gene delivery of c-myb increases bone formation surrounding oral implants.
abstract: Bone regeneration around titanium (Ti) implants is a relatively slow process. The c-myb transcription factor has been associated with high proliferation and differentiation rates in bone. This study analyzed whether c-myb can enhance new bone surrounding the implant. In vitro overexpressed chitosan-gold nanoparticles conjugated with plasmid DNA/c-myb (Ch-GNPs/c-myb)-coated Ti surfaces were associated with enhanced expression of the osteogenic molecules osteopontin (OPN), runt-related transcription factor 2 (RUNX-2), and bone morphogenetic proteins (BMP2/7) in MC-3T3E1 osteoblast cells. Further, to determine its in vivo effect, we inserted Ch-GNPs/c-myb-coated Ti implants into rat mandibles. One and 4 wks post-implantation, mandibles were examined by microcomputed tomography, immunohistochemistry, and hematoxylin & eosin staining. The microcomputed tomography analysis demonstrated that c-myb overexpression increased the density and volume of newly formed bone surrounding the implants, compared with those in controls (p < .05). Further, c-myb increased the number of cells expressing BMP2/7 and aided in the increase of new bone (p < .05). These results support the view that c-myb overexpression accelerates new bone surrounding implants and can serve as a potent molecule in promoting tissue regeneration around dental implants. The recipient rat used in this system provides an excellent in vivo model for studies of bone regeneration.
authors.affiliation: Department of Oral Biochemistry, BK21 program, School of Dentistry, Chonbuk National University, Jeonju, Korea.
authors.forename: G
authors.initials: G
authors.lastname: Bhattarai
authors.forename: Y H
authors.initials: YH
authors.lastname: Lee
authors.forename: M H
authors.initials: MH
authors.lastname: Lee
authors.forename: H K
authors.initials: HK
authors.lastname: Yi
mesh_terms: D016475:3T3 Cells; D000818:Animals; D055396:Bone Morphogenetic Protein 2 / Q000187:drug effects; D055419:Bone Morphogenetic Protein 7 / Q000187:drug effects; D001861:Bone Regeneration / Q000187:drug effects; D048271:Chitosan / Q000737:chemistry; D020099:Coated Materials, Biocompatible / Q000494:pharmacology*; D050659:Core Binding Factor Alpha 1 Subunit / Q000187:drug effects; D015921:Dental Implants; D003764:Dental Materials / Q000737:chemistry*; D018014:Gene Transfer Techniques; D006046:Gold / Q000737:chemistry; D008297:Male; D008334:Mandible / Q000187:drug effects* / Q000473:pathology / Q000601:surgery; D051379:Mice; D053758:Nanoparticles / Q000737:chemistry; D010006:Osteoblasts / Q000187:drug effects; D010012:Osteogenesis / Q000187:drug effects*; D053495:Osteopontin / Q000187:drug effects; D020598:Proto-Oncogene Proteins c-myb / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014025:Titanium / Q000737:chemistry; D020390:Tooth Socket / Q000187:drug effects / Q000473:pathology / Q000601:surgery
chemical_list: C525719:Bmp2 protein, mouse; C525720:Bmp2 protein, rat; C525821:Bmp7 protein, rat; D055396:Bone Morphogenetic Protein 2; D055419:Bone Morphogenetic Protein 7; D020099:Coated Materials, Biocompatible; D050659:Core Binding Factor Alpha 1 Subunit; D015921:Dental Implants; D003764:Dental Materials; D020598:Proto-Oncogene Proteins c-myb; C493716:Runx2 protein, mouse; C493717:Runx2 protein, rat; C506896:Spp1 protein, mouse; C506897:Spp1 protein, rat; C525820:bmp7 protein, mouse; D053495:Osteopontin; D006046:Gold; D048271:Chitosan; D014025:Titanium
keywords: bone regeneration; chitosan gold nanoparticles; dental implant; osteoblast; osteogenesis; titanium