title: Highly efficient genome modifications mediated by CRISPR/Cas9 in Drosophila.
abstract: We report that Cas9/gRNA mediates efficient genetic modifications in Drosophila. Through targeting seven loci, we achieved a germline efficiency of up to 100%. Genes in both heterochromatin and euchromatin can be modified efficiently. Thus the Cas9/gRNA system is an attractive tool for rapid disruption of essentially any gene in Drosophila.
authors.affiliation: State Key Laboratory of Brain and Cognitive Science, Institute of Biophysics, Chinese Academy of Sciences, Datun Road 15, Beijing 100101, China.
authors.forename: Zhongsheng
authors.initials: Z
authors.lastname: Yu
authors.forename: Mengda
authors.initials: M
authors.lastname: Ren
authors.forename: Zhanxiang
authors.initials: Z
authors.lastname: Wang
authors.forename: Bo
authors.initials: B
authors.lastname: Zhang
authors.forename: Yikang S
authors.initials: YS
authors.lastname: Rong
authors.forename: Renjie
authors.initials: R
authors.lastname: Jiao
authors.forename: Guanjun
authors.initials: G
authors.lastname: Gao
mesh_terms: D000818:Animals; D064113:CRISPR-Cas Systems; D004330:Drosophila / Q000235:genetics*; D018390:Gene Targeting / Q000379:methods*; D049750:Genome, Insect
keywords: CRISPR/Cas9; Drosophila; NHEJ; gRNA; genome modifications