title: Periodontal tissue regeneration by combined implantation of adipose tissue-derived stem cells and platelet-rich plasma in a canine model.
abstract: OBJECTIVE
  One goal of periodontal therapy is to regenerate periodontal tissues. Stem cells, growth factors and scaffolds and biomaterials are vital for the restoration of the architecture and function of complex tissues. Adipose tissue-derived stem cells (ASCs) are an ideal population of stem cells for practical regenerative medicine. In addition, platelet-rich plasma (PRP) can be useful for its ability to stimulate tissue regeneration. PRP contains various growth factors and may be useful as a cell carrier in stem cell therapies. The purpose of this study was to determine whether a mixture of ASCs and PRP promoted periodontal tissue regeneration in a canine model.
  
  
  METHODS
  Autologous ASCs and PRP were implanted into areas with periodontal tissue defects. Periodontal tissue defects that received PRP alone or non-implantation were also examined. Histologic, immunohistologic and x-ray studies were performed 1 or 2 months after implantation. The amount of newly formed bone and the scale of newly formed cementum in the region of the periodontal tissue defect were analyzed on tissue sections.
  
  
  RESULTS
  The areas of newly formed bone and cementum were greater 2 months after implantation of ASCs and PRP than at 1 month after implantation, and the radiopacity in the region of the periodontal tissue defect increased markedly by 2 months after implantation. The ASCs and PRP group exhibited periodontal tissue with the correct architecture, including alveolar bone, cementum-like structures and periodontal ligament-like structures, by 2 months after implantation.
  
  
  CONCLUSIONS
  These findings suggest that a combination of autologous ASCs and PRP promotes periodontal tissue regeneration that develops the appropriate architecture for this complex tissue.
authors.affiliation: Department of Plastic and Reconstructive Surgery, Juntendo University School of Medicine, Tokyo, Japan.
authors.forename: Morikuni
authors.initials: M
authors.lastname: Tobita
authors.forename: Cagri A
authors.initials: CA
authors.lastname: Uysal
authors.forename: Xin
authors.initials: X
authors.lastname: Guo
authors.forename: Hiko
authors.initials: H
authors.lastname: Hyakusoku
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Mizuno
mesh_terms: D000273:Adipose Tissue / Q000166:cytology; D000818:Animals; D001861:Bone Regeneration; D003739:Dental Cementum / Q000637:transplantation; D004285:Dogs; D016557:Guided Tissue Regeneration, Periodontal; D045164:Mesenchymal Stem Cell Transplantation; D010510:Periodontal Diseases / Q000628:therapy*; D053657:Platelet-Rich Plasma / Q000737:chemistry / Q000378:metabolism; D054457:Tissue Scaffolds; D014945:Wound Healing
keywords: adipose-derived stem cells; adult stem cells; canine; periodontal disease; periodontal tissue regeneration; platelet-rich plasma