title: Mg ion implantation on SLA-treated titanium surface and its effects on the behavior of mesenchymal stem cell.
abstract: Magnesium (Mg) is one of the most important ions associated with bone osseointegration. The aim of this study was to evaluate the cellular effects of Mg implantation in titanium (Ti) surfaces treated with sand blast using large grit and acid etching (SLA). Mg ions were implanted into the surface via vacuum arc source ion implantation. The surface morphology, chemical properties, and the amount of Mg ion release were evaluated by scanning electron microscopy (SEM), Auger electron spectroscopy (AES), Rutherford backscattering spectroscopy (RBS), and inductively coupled plasma-optical emission spectrometer (ICP-OES). Human mesenchymal stem cells (hMSCs) were used to evaluate cellular parameters such as proliferation, cytotoxicity, and adhesion morphology by MTS assay, live/dead assay, and SEM. Furthermore, osteoblast differentiation was determined on the basis of alkaline phosphatase (ALP) activity and the degree of calcium accumulation. In the Mg ion-implanted disk, 2.3×10(16) ions/cm(2) was retained. However, after Mg ion implantation, the surface morphology did not change. Implanted Mg ions were rapidly released during the first 7 days in vitro. The MTS assay, live/dead assay, and SEM demonstrated increased cell attachment and growth on the Mg ion-implanted surface. In particular, Mg ion implantation increased the initial cell adhesion, and in an osteoblast differentiation assay, ALP activity and calcium accumulation. These findings suggest that Mg ion implantation using the plasma source ion implantation (PSII) technique may be useful for SLA-treated Ti dental implants to improve their osseointegration capacity.
authors.affiliation: Wonkwang Bone Regeneration Research Institute, Wonkwang University, Iksan 570-749, Republic of Korea.
authors.forename: Beom-Su
authors.initials: BS
authors.lastname: Kim
authors.forename: Jin Seong
authors.initials: JS
authors.lastname: Kim
authors.forename: Young Min
authors.initials: YM
authors.lastname: Park
authors.forename: Bo-Young
authors.initials: BY
authors.lastname: Choi
authors.forename: Jun
authors.initials: J
authors.lastname: Lee
mesh_terms: D000134:Acid Etching, Dental / Q000379:methods*; D000469:Alkaline Phosphatase / Q000378:metabolism; D002118:Calcium / Q000378:metabolism; D002448:Cell Adhesion / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D002478:Cells, Cultured; D006801:Humans; D007477:Ions; D008274:Magnesium / Q000494:pharmacology*; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000201:enzymology / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning; D008856:Microscopy, Fluorescence; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects / Q000648:ultrastructure; D013194:Staining and Labeling; D013499:Surface Properties; D014025:Titanium / Q000494:pharmacology*
chemical_list: D007477:Ions; D014025:Titanium; D000469:Alkaline Phosphatase; D008274:Magnesium; D002118:Calcium
keywords: Magnesium; Mesenchymal stem cells; Mg ion implanted titanium implant treated with sand blast with large grit and acid etching; Mg-SLA Ti; PSII; SLA; SLA Ti; Surface modification; Titanium implant; Vacuum arc source ion implantation; plasma source ion implantation; sand blast with large grit and acid etching; titanium implant treated with sand blast with large grit and acid etching