title: ¹H, ¹³C and ¹⁵N backbone and side-chain resonance assignments of a family 36 carbohydrate binding module of xylanase from Paenibacillus campinasensis.
abstract: Paenibacillus campinasensis BL11 isolated from black liquor secretes multiple glycoside hydrolases (GHs) against all kinds of polysaccharides. GH consists of a catalytic module and non-catalytic carbohydrate-binding modules (CBMs), in which CBMs append to the catalytic module, mediating specific interactions with insoluble carbohydrates to promote the hydrolysis efficiency of the cognate enzyme. Endo-β-1,4-xylanase (XylX) is one of the GHs reveals high enzymatic activity in a wide range of pH and thermal endurance, suitable for bioconversion and bio-refinement applications. In this work, we report the resonance assignments of a family 36 CBM (characterized as CBM36) derived from XylX. Our investigations will facilitate molecular structure determination and molecular dynamics analysis of CBMs.
authors.affiliation: School of Forestry and Resource Conservation, National Taiwan University, Taipei, Taiwan, ROC.
authors.forename: Yu-Sheng
authors.initials: YS
authors.lastname: Wang
authors.forename: Chun-Han
authors.initials: CH
authors.lastname: Ko
authors.forename: Hao-Ting
authors.initials: HT
authors.lastname: Chang
authors.forename: Kai-Jay
authors.initials: KJ
authors.lastname: Yang
authors.forename: Yu-Jen
authors.initials: YJ
authors.lastname: Chen
authors.forename: Shing-Jong
authors.initials: SJ
authors.lastname: Huang
authors.forename: Pei-Ju
authors.initials: PJ
authors.lastname: Fang
authors.forename: Chi-Fon
authors.initials: CF
authors.lastname: Chang
authors.forename: Der-Lii M
authors.initials: DL
authors.lastname: Tzou
mesh_terms: D000595:Amino Acid Sequence; D050260:Carbohydrate Metabolism; D043364:Endo-1,4-beta Xylanases / Q000737:chemistry* / Q000378:metabolism*; D019906:Nuclear Magnetic Resonance, Biomolecular; D056507:Paenibacillus / Q000201:enzymology*
chemical_list: D043364:Endo-1,4-beta Xylanases