title: Transgelin Up-Regulation in Obstructive Nephropathy.
abstract: Fibrosis is a complex and multifactorial process, affecting the structure and compromising the function of several organs. Among those, renal fibrosis is an important pathological change, eventually leading to renal failure. Proteomic analysis of the renal parenchyma in the well-established rat model of unilateral ureteral obstruction (UUO model) suggested that transgelin was up-regulated during the development of fibrosis. Transgelin up-regulation was confirmed both at the protein and at the mRNA level. It was observed that at early stages of fibrosis transgelin was mainly expressed in the interstitial compartment and, more specifically, in cells surrounding the glomeruli. Subsequently, it was confirmed that transgelin expressing cells were activated fibroblasts, based on their extensive co-expression of α-SMA and their complete lack of co-distribution with markers of other cell types (endothelial, epithelial and cells of the immune system). These periglomerular fibroblasts exhibited staining for transgelin mainly cytoplasmic but occasionally nuclear as well. In addition, transgelin expression in periglomerular fibroblasts was absent in renal fibrosis developed in a hypertensive model, compared to the UUO model. Promoter analysis indicated that there are several conserved motifs for transcription factor binding. Among those, Kruppel-like factor 6 was found to be up-regulated in transgelin positive periglomerular activated fibroblasts, suggesting a possible involvement in the mechanism of transgelin up-regulation. These data strongly suggest that transgelin is up-regulated in the obstructive nephropathy and could be used as a novel marker for renal fibrosis in the future.
authors.affiliation: Biomedical Research Foundation of the Academy of Athens, Section of Histology, Center for Basic Research I, Athens, Greece.
authors.forename: Fani
authors.initials: F
authors.lastname: Karagianni
authors.forename: Niki
authors.initials: N
authors.lastname: Prakoura
authors.forename: Garyfallia
authors.initials: G
authors.lastname: Kaltsa
authors.forename: Panagiotis
authors.initials: P
authors.lastname: Politis
authors.forename: Elena
authors.initials: E
authors.lastname: Arvaniti
authors.forename: Valeria
authors.initials: V
authors.lastname: Kaltezioti
authors.forename: Stelios
authors.initials: S
authors.lastname: Psarras
authors.forename: Stamatis
authors.initials: S
authors.lastname: Pagakis
authors.forename: Michalis
authors.initials: M
authors.lastname: Katsimboulas
authors.forename: Ahmed
authors.initials: A
authors.lastname: Abed
authors.forename: Christos
authors.initials: C
authors.lastname: Chatziantoniou
authors.forename: Aristidis
authors.initials: A
authors.lastname: Charonis
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D005355:Fibrosis; D042541:Intracellular Space / Q000378:metabolism; D007668:Kidney / Q000473:pathology; D007674:Kidney Diseases / Q000150:complications*; D008297:Male; D008840:Microfilament Proteins / Q000235:genetics* / Q000378:metabolism*; D009124:Muscle Proteins / Q000235:genetics* / Q000378:metabolism*; D011401:Promoter Regions, Genetic / Q000235:genetics; D021381:Protein Transport; D051381:Rats; D017208:Rats, Wistar; D015854:Up-Regulation; D014517:Ureteral Obstruction / Q000150:complications / Q000235:genetics* / Q000378:metabolism* / Q000473:pathology
chemical_list: D015415:Biomarkers; D008840:Microfilament Proteins; D009124:Muscle Proteins; C051615:transgelin