title: No association between simian virus 40 and diffuse malignant mesothelioma of the pleura in Iranian patients: a molecular and epidemiologic case-control study of 60 patients.
abstract: BACKGROUND
  Diffuse malignant mesothelioma (DMM) is increasing in incidence on a worldwide basis and is linked to exposure to asbestos. Simian virus 40 (SV40), a DNA virus, was introduced inadvertently to human populations through contaminated polio vaccine during the years 1956-1963. It has been associated with various types of malignancy in animal experiments. There have been suggestions that SV40 might play a role in the pathogenesis of DMM.
  
  
  OBJECTIVE
  To evaluate the association between SV40 and DMM in Iranian patients.
  
  
  METHODS
  In a case-control study between the years 2007-2008, isolated DNA from 60 paraffin blocks of patients with DMM and 60 controls was assessed to detect three human polyomaviruses (JCV, BKV, and SV40) using three different sets of primers by multiplex nested PCR analysis. We related the patients with diffuse malignant mesothelioma to possible sites of exposure to asbestos.
  
  
  RESULTS
  None of the DMMs nor any patient in the control group had SV40 genome on polymerase chain reaction (PCR). All of the cases were SV40 T antigen negative.
  
  
  CONCLUSIONS
  This study suggests that DMM is independent of SV40 infection in Iran.
authors.affiliation: Virology Research Center, National Research Institute of Tuberculosis and Lung Disease, Shahid Beheshti University of Medical Sciences, Tehran, Iran. zohrehmt@yahoo.com
authors.forename: Zohreh
authors.initials: Z
authors.lastname: Mohammad-Taheri
authors.forename: Seyed Alireza
authors.initials: SA
authors.lastname: Nadji
authors.forename: Farshid
authors.initials: F
authors.lastname: Raisi
authors.forename: Forouzan
authors.initials: F
authors.lastname: Mohammadi
authors.forename: Moslem
authors.initials: M
authors.lastname: Bahadori
authors.forename: Eugene Jerome
authors.initials: EJ
authors.lastname: Mark
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D007492:Iran / Q000453:epidemiology; D008297:Male; D008654:Mesothelioma / Q000453:epidemiology / Q000821:virology*; D008875:Middle Aged; D010997:Pleural Neoplasms / Q000453:epidemiology / Q000821:virology*; D016133:Polymerase Chain Reaction; D027601:Polyomavirus Infections / Q000453:epidemiology / Q000821:virology*; D013539:Simian virus 40 / Q000235:genetics; D014412:Tumor Virus Infections / Q000453:epidemiology / Q000821:virology*
keywords: PCR; etiology; mesothelioma malignant; simian virus 40