title: Induction of ankylosis in the incisor for orthodontic tooth movement in rats.
abstract: OBJECTIVE
  The present research evaluated the induction of ankylosis and the eruption rate in rat incisors, with the aim of achieving stable anchorage for orthodontic tooth movement (OTM) of the molars.
  
  
  METHODS
  Fifteen male Wistar rats were used. In the Experimental group, the right superior incisor of each animal was extracted, denuded of the PDL and the dental papilla, and reimplanted. The Control group was the left superior incisors. The eruption rate was measured at 7, 10, 12, 14, and 16 days after replantation. NiTi closed springs with a 50cN release force were installed for additional 9 days, and the eruption rate was determined. Then, the rats were sacrificed, and ankylosis was examined by microscopic analysis. Differences with P < 0.01 were defined as statistically significant.
  
  
  RESULTS
  The eruption rates were 0 and 0.39 mm day(-1) in the Experimental group and Control group, respectively. All incisors of the Experimental group showed ankylosis according to histological analysis.
  
  
  CONCLUSIONS
  The methodology used to induce ankylosis in this study was effective for anchoring the incisors during the OTM of rat molars, eliminating the undesirable effects consequent to continuous eruption.
authors.affiliation: Department of Pediatric and Community Dentistry, UNESP - Univ Estadual Paulista, Araçatuba, Brazil.
authors.forename: Osmar Aparecido
authors.initials: OA
authors.lastname: Cuoghi
authors.forename: Pedro Marcelo
authors.initials: PM
authors.lastname: Tondelli
authors.forename: Celso Koogi
authors.initials: CK
authors.lastname: Sonoda
authors.forename: Carlos Alberto
authors.initials: CA
authors.lastname: Aiello
authors.forename: Marcos Rogério
authors.initials: MR
authors.lastname: de Mendonça
authors.forename: Silvano Cesar da
authors.initials: SC
authors.lastname: Costa
mesh_terms: D000818:Animals; D007180:Incisor; D008297:Male; D009970:Orthodontics; D051381:Rats; D017208:Rats, Wistar; D020254:Tooth Ankylosis; D014078:Tooth Eruption / Q000502:physiology; D014087:Tooth Movement Techniques / Q000295:instrumentation*
keywords: Ankylosis; Periodontal ligament; Rats; Tooth eruption; Tooth movement; Tooth replantation