title: Simultaneous imaging of cortical blood flow and haemoglobin concentration with LASCA and RGB reflectometry.
abstract: We demonstrate a system for the simultaneous imaging of cortical blood flow and haemoglobin oxygenation by laser speckle contrast analysis (LASCA) and RGB reflectometry. The sensitivity of the system was tested by observing changes of haemoglobin oxygenation and blood flow in rats in response to ischaemic stroke, hypercapnia, hyperoxia, hypoxia, cortical spreading depression and cortical activation following forepaw stimulation.
authors.affiliation: RheinAhr Campus Remagen, University of Applied Sciences Koblenz, Remagen, Germany. steimers@rheinahrcampus.de.
authors.forename: André
authors.initials: A
authors.lastname: Steimers
authors.affiliation: Max Planck Institute for Neurological Research, Cologne, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Gramer
authors.affiliation: Max Planck Institute for Neurological Research, Cologne, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Takagaki
authors.affiliation: Max Planck Institute for Neurological Research, Cologne, Germany.
authors.forename: R
authors.initials: R
authors.lastname: Graf
authors.affiliation: Department of Neurosurgery, Klinikum rechts der Isar, Technical University Munich, Munich, Germany.
authors.forename: U
authors.initials: U
authors.lastname: Lindauer
authors.affiliation: RheinAhr Campus Remagen, University of Applied Sciences Koblenz, Remagen, Germany.
authors.forename: Matthias
authors.initials: M
authors.lastname: Kohl-Bareis
mesh_terms: D000818:Animals; D002540:Cerebral Cortex / Q000098:blood supply*; D002560:Cerebrovascular Circulation / Q000502:physiology*; D013181:Cortical Spreading Depression / Q000502:physiology; D003952:Diagnostic Imaging / Q000379:methods; D006454:Hemoglobins / Q000378:metabolism*; D006935:Hypercapnia / Q000378:metabolism / Q000503:physiopathology; D018496:Hyperoxia / Q000378:metabolism / Q000503:physiopathology; D007834:Lasers; D010100:Oxygen / Q000378:metabolism; D051381:Rats; D020521:Stroke / Q000378:metabolism / Q000503:physiopathology
chemical_list: D006454:Hemoglobins; D010100:Oxygen