title: Polymorphism in tropomyosin structure and function.
abstract: Tropomyosins (Tm) in humans are expressed from four distinct genes and by alternate splicing >40 different Tm polypeptide chains can be made. The functional Tm unit is a dimer of two parallel polypeptide chains and these can be assembled from identical (homodimer) or different (heterodimer) polypeptide chains provided both chains are of the same length. Since most cells express multiple isoforms of Tm, the number of different homo and heterodimers that can be assembled becomes very large. We review the mechanism of dimer assembly and how preferential assembly of some heterodimers is driven by thermodynamic stability. We examine how in vitro studies can reveal functional differences between Tm homo and heterodimers (stability, actin affinity, flexibility) and the implication for how there could be selection of Tm isomers in the assembly on to an actin filament. The role of Tm heterodimers becomes more complex when mutations in Tm are considered, such as those associated with cardiomyopathies, since mutations can appear in only one of the chains.
authors.affiliation: School of Biosciences, University of Kent, Canterbury, Kent, UK.
authors.forename: Miro
authors.initials: M
authors.lastname: Janco
authors.forename: Worawit
authors.initials: W
authors.lastname: Suphamungmee
authors.forename: Xiaochuan
authors.initials: X
authors.lastname: Li
authors.forename: William
authors.initials: W
authors.lastname: Lehman
authors.forename: Sherwin S
authors.initials: SS
authors.lastname: Lehrer
authors.forename: Michael A
authors.initials: MA
authors.lastname: Geeves
mesh_terms: D000199:Actins / Q000737:chemistry / Q000378:metabolism; D000818:Animals; D006801:Humans; D011110:Polymorphism, Genetic; D020033:Protein Isoforms; D013329:Structure-Activity Relationship; D014335:Tropomyosin / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism
chemical_list: D000199:Actins; D020033:Protein Isoforms; D014335:Tropomyosin