title: New type of antimicrobial protein produced by the plant pathogen Clavibacter michiganensis subsp. michiganensis.
abstract: It has previously been shown that the tomato pathogen Clavibacter michiganensis subsp. michiganensis secretes a 14-kDa protein, C. michiganensis subsp. michiganensis AMP-I (CmmAMP-I), that inhibits growth of Clavibacter michiganensis subsp. sepedonicus, the causal agent of bacterial ring rot of potato. Using sequences obtained from tryptic fragments, we have identified the gene encoding CmmAMP-I and we have recombinantly produced the protein with an N-terminal intein tag. The gene sequence showed that CmmAMP-I contains a typical N-terminal signal peptide for Sec-dependent secretion. The recombinant protein was highly active, with 50% growth inhibition (IC50) of approximately 10 pmol, but was not toxic to potato leaves or tubers. CmmAMP-I does not resemble any known protein and thus represents a completely new type of bacteriocin. Due to its high antimicrobial activity and its very narrow inhibitory spectrum, CmmAMP-1 may be of interest in combating potato ring rot disease.
authors.affiliation: Department of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences, Ås, Norway.
authors.forename: Zhanliang
authors.initials: Z
authors.lastname: Liu
authors.forename: Ping
authors.initials: P
authors.lastname: Ma
authors.forename: Ingrid
authors.initials: I
authors.lastname: Holtsmark
authors.forename: Morten
authors.initials: M
authors.lastname: Skaugen
authors.forename: Vincent G H
authors.initials: VG
authors.lastname: Eijsink
authors.forename: May B
authors.initials: MB
authors.lastname: Brurberg
mesh_terms: D000192:Actinomycetales / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism*; D000890:Anti-Infective Agents / Q000378:metabolism* / Q000633:toxicity; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism* / Q000633:toxicity; D020128:Inhibitory Concentration 50; D018551:Solanum lycopersicum; D008826:Microbial Sensitivity Tests; D018515:Plant Leaves / Q000187:drug effects; D018517:Plant Roots / Q000187:drug effects; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism / Q000633:toxicity; D011198:Solanum tuberosum / Q000187:drug effects
chemical_list: D000890:Anti-Infective Agents; D001426:Bacterial Proteins; D011994:Recombinant Proteins