title: Changing profile of rotavirus genotypes in Bangladesh, 2006-2012.
abstract: BACKGROUND
  Rotavirus is the leading cause of severe diarrhea in infants and young children worldwide including Bangladesh. Unlike what was seen in high-income countries, the licensed rotavirus vaccines did not show high efficacy in Bangladeshi trials. We assessed rotavirus prevalence and genotypes in Bangladesh over six-year period to provide baseline information on the rotavirus burden and changing profile in the country.
  
  
  METHODS
  This study was conducted from June 2006 to May 2012 in Matlab, Bangladesh. Group A rotaviruses were detected in stools collected from diarrhea patients by ELISA and genotyped using multiplex reverse transcription PCR followed by nucleotide sequencing.
  
  
  RESULTS
  Of the 9678 stool samples, 20.3% were positive for rotavirus. The most predominant genotype was G1P[8] (22.4%), followed by G9P[8] (20.8%), G2P[4] (16.9%) and G12P[8] (10.4%). Mixed infections were detected in 14.2% of the samples. Emergence of an unusual strain, G9P[4] was documented during 2011-12. Several amino acid mismatches in the antigenic epitopes of VP7 and VP4 between Bangladeshi and the vaccine strains were identified.
  
  
  CONCLUSIONS
  Our study provides important information on rotavirus genotypes that should be considered for the selection and introduction of rotavirus vaccines in Bangladesh.
authors.affiliation: Virology Laboratory, International Centre for Diarrhoeal Disease Research, Bangladesh (icddr,b), Mohakhali, Dhaka 1212, Bangladesh.
authors.forename: Mokibul Hassan
authors.initials: MH
authors.lastname: Afrad
authors.forename: Zahid
authors.initials: Z
authors.lastname: Hassan
authors.forename: Saiada
authors.initials: S
authors.lastname: Farjana
authors.forename: Sayra
authors.initials: S
authors.lastname: Moni
authors.forename: Subarna
authors.initials: S
authors.lastname: Barua
authors.forename: Sumon Kumar
authors.initials: SK
authors.lastname: Das
authors.forename: Abu Syed Golam
authors.initials: AS
authors.lastname: Faruque
authors.forename: Tasnim
authors.initials: T
authors.lastname: Azim
authors.forename: Mustafizur
authors.initials: M
authors.lastname: Rahman
mesh_terms: D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D000956:Antigens, Viral / Q000276:immunology; D001459:Bangladesh / Q000453:epidemiology; D003967:Diarrhea / Q000453:epidemiology / Q000821:virology; D005243:Feces / Q000821:virology; D005838:Genotype; D006801:Humans; D008969:Molecular Sequence Data; D011446:Prospective Studies; D012401:Rotavirus / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification; D012400:Rotavirus Infections / Q000453:epidemiology / Q000821:virology*; D022243:Rotavirus Vaccines / Q000008:administration & dosage / Q000276:immunology
chemical_list: D000956:Antigens, Viral; D022243:Rotavirus Vaccines