title: Ovine HSP90AA1 expression rate is affected by several SNPs at the promoter under both basal and heat stress conditions.
abstract: The aim of this work was to investigate the association between polymorphisms located at the HSP90AA1 ovine gene promoter and gene expression rate under different environmental conditions, using a mixed model approach. Blood samples from 120 unrelated rams of the Manchega sheep breed were collected at three time points differing in environmental conditions. Rams were selected on the basis of their genotype for the transversion G/C located 660 base pairs upstream the gene transcription initiation site. Animals were also genotyped for another set of 6 SNPs located at the gene promoter. Two SNPs, G/C-660 and A/G-444, were associated with gene overexpression resulting from heat stress. The composed genotype CC-660-AG-444 was the genotype having the highest expression rates with fold changes ranging from 2.2 to 3.0. The genotype AG-522 showed the highest expression levels under control conditions with a fold change of 1.4. Under these conditions, the composed genotype CC-601-TT-524-AG-522-TT-468 is expected to be correlated with higher basal expression of the gene according to genotype frequencies and linkage disequilibrium values. Some putative transcription factors were predicted for binding sites where the SNPs considered are located. Since the expression rate of the gene under alternative environmental conditions seems to depend on the composed genotype of several SNPs located at its promoter, a cooperative regulation of the transcription of the HSP90AA1 gene could be hypothesized. Nevertheless epigenetic regulation mechanisms cannot be discarded.
authors.affiliation: Dpto. Mejora Genética animal. Inst. Nac. Invest. Agrarias y Alimentarias, Madrid, Spain. malena@inia.es
authors.forename: Judit
authors.initials: J
authors.lastname: Salces-Ortiz
authors.forename: Carmen
authors.initials: C
authors.lastname: González
authors.forename: Natalia
authors.initials: N
authors.lastname: Moreno-Sánchez
authors.forename: Jorge H
authors.initials: JH
authors.lastname: Calvo
authors.forename: M Dolores
authors.initials: MD
authors.lastname: Pérez-Guzmán
authors.forename: Magdalena M
authors.initials: MM
authors.lastname: Serrano
mesh_terms: D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D005786:Gene Expression Regulation; D018841:HSP90 Heat-Shock Proteins / Q000235:genetics*; D018869:Heat-Shock Response / Q000235:genetics*; D015810:Linkage Disequilibrium; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic; D012756:Sheep
chemical_list: D017931:DNA Primers; D018841:HSP90 Heat-Shock Proteins