title: [Effects of Ac-SDKP on angiotensin II-induced collagen synthesis in vascular adventitial fibroblasts].
abstract: OBJECTIVE
  To investigate the effect of an anti-fibrotic tetra peptide Ac-SDKP on vascular fibrosis by regulating extracellular regulated protein kinase (ERK1/2) activity through Ang II.
  
  
  METHODS
  Rat vascular adventitial fibroblasts were cultured in vitro. They were randomly divided into control group, Ang II (10(-6) mmol/L) group, Ang II and Ac-SDKP joint action group, PD98059 group. Type I, III collagen contents in adventitia fibroblasts were measured by RT-PCR and the expressions of matrix metalloproteinases (MMP-2) and transforming growth factor-beta1 (TGF-beta1) were determined by Western blot.
  
  
  RESULTS
  Ac-SDKP could reduced Ang II-induced expression of type I, III collagen secretion and TGF-beta1 at mRNA,and increase MMP-2 expression, PD98059 could inhibit the above effect.
  
  
  CONCLUSIONS
  The results suggested that Ac-SDKP could inhibit the formation and development of vascular fibrosis through blocking ERK1/2 pathway mediated by Ang II. Ac-SDKP therefore served as an antifibrotic factor in vascular fibrosis.
authors.affiliation: Department of Clinical Medicine, Wannan Medical College, Wuhu 241000, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Wang
authors.forename: Xiang-Quan
authors.initials: XQ
authors.lastname: Kong
authors.forename: Wei-Hua
authors.initials: WH
authors.lastname: Wang
mesh_terms: D000804:Angiotensin II / Q000009:adverse effects; D000818:Animals; D002478:Cells, Cultured; D024042:Collagen Type I / Q000096:biosynthesis*; D024061:Collagen Type III / Q000096:biosynthesis*; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D005419:Flavonoids / Q000494:pharmacology; D020935:MAP Kinase Signaling System; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D009842:Oligopeptides / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D053773:Transforming Growth Factor beta1 / Q000378:metabolism
chemical_list: D024042:Collagen Type I; D024061:Collagen Type III; D005419:Flavonoids; D009842:Oligopeptides; D053773:Transforming Growth Factor beta1; D000804:Angiotensin II; D020778:Matrix Metalloproteinase 2; C522363:Mmp2 protein, rat; C058504:goralatide; C093973:2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one