title: The longest mitochondrial RNA editing PPR protein MEF12 in Arabidopsis thaliana requires the full-length E domain.
abstract: Mitochondrial RNA editing factor 12 (MEF12) was identified in a screen for editing defects of a chemically mutated plant population in Arabidopsis thaliana. The MEF12 editing protein is required for the C to U change of nucleotide nad5-374. The MEF12 polypeptide is characterized by an exceptionally long stretch of 25 pentatricopeptide repeats (PPR) and a C-terminal extension domain. Editing is lost in mutant plants with a stop codon in the extending element. A T-DNA insertion substituting the 10 C-terminal amino acids of the extension domain reduces RNA editing to about 20% at the target site in a mutant plant. These results support the importance of the full-length extension module for functional RNA editing in plant mitochondria.
authors.affiliation: Molekulare Botanik; Universität Ulm; Ulm, Germany.
authors.forename: Barbara
authors.initials: B
authors.lastname: Härtel
authors.affiliation: Molekulare Botanik; Universität Ulm; Ulm, Germany.
authors.forename: Anja
authors.initials: A
authors.lastname: Zehrmann
authors.affiliation: Molekulare Botanik; Universität Ulm; Ulm, Germany.
authors.forename: Daniil
authors.initials: D
authors.lastname: Verbitskiy
authors.affiliation: Molekulare Botanik; Universität Ulm; Ulm, Germany.
authors.forename: Mizuki
authors.initials: M
authors.lastname: Takenaka
mesh_terms: D017360:Arabidopsis / Q000235:genetics*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant; D009154:Mutation; D017434:Protein Structure, Tertiary; D012313:RNA / Q000378:metabolism; D017393:RNA Editing; D000077278:RNA, Mitochondrial; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; C000597737:MEF12 protein, Arabidopsis; D000077278:RNA, Mitochondrial; D016601:RNA-Binding Proteins; D012313:RNA
keywords: E-domain; MEF12; PPR protein; RNA editing factor; plant mitochondria