title: Mutational analysis of cj0183 Campylobacter jejuni promoter.
abstract: Gene-nominated cj0183 was identified in Campylobacter jejuni NCTC 11168 and in two human isolates 81116 and 81-176. It encodes a protein which shows partial homology to TlyC of Brachyspira hyodysenteriae. The aim of this work was to determine the mechanisms of gene regulation by cloning DNA fragments lying upstream of the cj0183 gene. The β-galactosidase activity determined for the strain harboring the plasmid with the fragment upstream of cj0183 indicated the presence of a promoter in this DNA region. Mutations in cj0183 -10 region, -16 region, and -35 region resulted in changes in gene transcription.
authors.affiliation: Department of Pre-Clinical Sciences, Warsaw University of Life Sciences, Warsaw, Poland, asalam@tlen.pl.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Salamasznska-Guz
authors.forename: Marta
authors.initials: M
authors.lastname: Grodzik
authors.forename: Danuta
authors.initials: D
authors.lastname: Klimuszko
mesh_terms: D019677:Artificial Gene Fusion; D001483:Base Sequence; D016123:Campylobacter jejuni / Q000235:genetics*; D004252:DNA Mutational Analysis; D015870:Gene Expression; D017930:Genes, Reporter; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D001616:beta-Galactosidase / Q000032:analysis / Q000235:genetics
chemical_list: D001616:beta-Galactosidase