title: Effect of furosemide and high-dosage pimobendan administration on the renin-angiotensin-aldosterone system in dogs.
abstract: OBJECTIVE
  To determine whether a high dosage of pimobendan, when administered concurrently with moderate-dosage furosemide to healthy dogs, would activate the renin-angiotensin-aldosterone system (RAAS) more than furosemide alone.
  
  
  METHODS
  12 healthy dogs.
  
  
  METHODS
  6 dogs received furosemide (2.0 mg/kg, PO, q 12 h) only, as an RAAS activator, for 10 days. The other 6 dogs received furosemide (2.0 mg/kg, PO, q 12 h) and pimobendan (0.6 mg/kg, PO, q 12 h) for 10 days. The effect of these drugs on the RAAS was determined by measurement of the aldosterone-to-creatinine ratio (A:C) in urine collected in the morning and evening of study days -2, -1, 1, 5, and 10.
  
  
  RESULTS
  Although there was an increase in the urine A:C during the study period in both groups, it was significant only for dogs that received both drugs. The urine A:C only differed significantly between groups on day 1, at which time A:C was greater in the group that received both drugs.
  
  
  CONCLUSIONS
  High-dosage pimobendan administration neither substantially suppressed nor potentiated the RAAS when administered with furosemide in healthy dogs.
authors.affiliation: Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, NC 27607, USA.
authors.forename: Marisa K
authors.initials: MK
authors.lastname: Ames
authors.forename: Clarke E
authors.initials: CE
authors.lastname: Atkins
authors.forename: Andrea C
authors.initials: AC
authors.lastname: Lantis
authors.forename: Stephen R
authors.initials: SR
authors.lastname: Werre
mesh_terms: D000818:Animals; D002316:Cardiotonic Agents / Q000494:pharmacology*; D004232:Diuretics / Q000494:pharmacology*; D004285:Dogs; D004305:Dose-Response Relationship, Drug; D004359:Drug Therapy, Combination; D005260:Female; D005665:Furosemide / Q000494:pharmacology*; D008297:Male; D011724:Pyridazines / Q000494:pharmacology*; D012084:Renin-Angiotensin System / Q000187:drug effects* / Q000502:physiology
chemical_list: D002316:Cardiotonic Agents; D004232:Diuretics; D011724:Pyridazines; C041648:pimobendan; D005665:Furosemide