title: Uncoupling of influenza A virus transcription and replication through mutation of the unpaired adenosine in the viral RNA promoter.
abstract: Transcription and replication of the influenza A virus RNA genome are mediated by the viral RNA polymerase from a promoter consisting of the partially base-paired 3' and 5' termini of viral genome segments. Here we show that transcription and replication can be uncoupled by mutation of an unpaired adenosine in the 5' strand of the promoter. This residue is important for transcription but not replication by being essential for the cap-binding activity of the RNA polymerase.
authors.affiliation: University of Oxford, Sir William Dunn School of Pathology, Oxford, United Kingdom.
authors.forename: Aartjan J W
authors.initials: AJ
authors.lastname: te Velthuis
authors.forename: Lauren
authors.initials: L
authors.lastname: Turrell
authors.forename: Frank T
authors.initials: FT
authors.lastname: Vreede
authors.forename: Ervin
authors.initials: E
authors.lastname: Fodor
mesh_terms: D000241:Adenosine / Q000235:genetics; D009980:Influenza A virus / Q000235:genetics / Q000502:physiology*; D017354:Point Mutation; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D012367:RNA, Viral / Q000096:biosynthesis* / Q000235:genetics; D012324:RNA-Dependent RNA Polymerase / Q000378:metabolism; D014158:Transcription, Genetic; D014779:Virus Replication
chemical_list: D012367:RNA, Viral; D012324:RNA-Dependent RNA Polymerase; D000241:Adenosine