title: Enhancement of in vitro cell motility and invasiveness of human malignant pleural mesothelioma cells through the HIF-1α-MUC1 pathway.
abstract: In this study, we examined the effects of hypoxia on the malignancy of human malignant pleural mesothelioma (MPM) cell lines, and found (1) hypoxia enhanced motility and invasiveness of human malignant pleural mesothelioma (MPM) cells; (2) this phenomenon resulted from increased expression of sialylated MUC1 through the activation of HIF-1 pathway; (3) two HIF-binding sites located in the promoter region of MUC1 were important for MUC1 transactivation under hypoxia. These findings are useful for better understanding molecular mechanisms of aggressive behavior of MPM cells and for targeting them in the clinical therapies for MPM patients.
authors.affiliation: Division of Stem Cell Biology, Institute for Genetic Medicine, Hokkaido University, Kita-15, Nishi-7, Kita-ku, Sapporo 060-0815, Japan.
authors.forename: Houman
authors.initials: H
authors.lastname: Goudarzi
authors.forename: Hisashi
authors.initials: H
authors.lastname: Iizasa
authors.forename: Masako
authors.initials: M
authors.lastname: Furuhashi
authors.forename: Seitaro
authors.initials: S
authors.lastname: Nakazawa
authors.forename: Rie
authors.initials: R
authors.lastname: Nakane
authors.forename: Shanshan
authors.initials: S
authors.lastname: Liang
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Hida
authors.forename: Kazuyoshi
authors.initials: K
authors.lastname: Yanagihara
authors.forename: Takanori
authors.initials: T
authors.lastname: Kubo
authors.forename: Koji
authors.initials: K
authors.lastname: Nakagawa
authors.forename: Masanobu
authors.initials: M
authors.lastname: Kobayashi
authors.forename: Tatsuro
authors.initials: T
authors.lastname: Irimura
authors.forename: Jun-Ichi
authors.initials: J
authors.lastname: Hamada
mesh_terms: D015687:Cell Hypoxia; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D055785:Gene Knockdown Techniques; D006801:Humans; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000378:metabolism*; D008654:Mesothelioma / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D018396:Mucin-1 / Q000235:genetics / Q000378:metabolism*; D009361:Neoplasm Invasiveness; D010997:Pleural Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D015398:Signal Transduction
chemical_list: D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D018396:Mucin-1
keywords: HIF; Hypoxia; Invasiveness; MUC1; Mesothelioma; Motility