title: Enzymatic assay for Cu(II) with horseradish peroxidase and its application in colorimetric logic gate.
abstract: We report an ultrasensitive and colorimetric assay for Cu(II) via enzymatic amplification strategy. The enzymatic activity of horseradish peroxidase (HRP) is strongly inhibited by Cu(I), which can be used indirectly to assay Cu(II). The limit of detection (LOD) is 0.37 nM, and the detection of 20 nM Cu(II) in solution can be achieved with naked eyes. This assay can be used to construct a colorimetric logic gate.
authors.forename: Yunlei
authors.initials: Y
authors.lastname: Xianyu
authors.forename: Kui
authors.initials: K
authors.lastname: Zhu
authors.forename: Wenwen
authors.initials: W
authors.lastname: Chen
authors.forename: Xuefei
authors.initials: X
authors.lastname: Wang
authors.forename: Hongmei
authors.initials: H
authors.lastname: Zhao
authors.forename: Jiashu
authors.initials: J
authors.lastname: Sun
authors.forename: Zhuo
authors.initials: Z
authors.lastname: Wang
authors.forename: Xingyu
authors.initials: X
authors.lastname: Jiang
mesh_terms: D055162:Biocatalysis; D015374:Biosensing Techniques / Q000379:methods*; D003124:Colorimetry; D003300:Copper / Q000032:analysis* / Q000737:chemistry / Q000494:pharmacology; D006735:Horseradish Peroxidase / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000378:metabolism*; D008128:Logic; D008958:Models, Molecular; D010084:Oxidation-Reduction; D011487:Protein Conformation; D014867:Water / Q000737:chemistry
chemical_list: D014867:Water; D003300:Copper; D006735:Horseradish Peroxidase