title: Mammalian adaptation in the PB2 gene of avian H5N1 influenza virus.
abstract: The substitution of glutamic acid (E) for lysine (K) at position 627 of the PB2 protein of avian H5N1 viruses has been identified as a virulence and host range determinant for infection of mammals. Here, we report that the E-to-K host-adaptive mutation in the PB2 gene appeared from day 4 and 5 along the respiratory tracts of mice and was complete by day 6 postinoculation. This mutation correlated with efficient replication of the virus in mice.
authors.affiliation: Laboratory of Infectious Diseases, NIAID, NIH, Bethesda, Maryland, USA.
authors.forename: Ji-Young
authors.initials: JY
authors.lastname: Min
authors.forename: Celia
authors.initials: C
authors.lastname: Santos
authors.forename: Adam
authors.initials: A
authors.lastname: Fitch
authors.forename: Alan
authors.initials: A
authors.lastname: Twaddle
authors.forename: Yoshiko
authors.initials: Y
authors.lastname: Toyoda
authors.forename: Jay V
authors.initials: JV
authors.lastname: DePasse
authors.forename: Elodie
authors.initials: E
authors.lastname: Ghedin
authors.forename: Kanta
authors.initials: K
authors.lastname: Subbarao
mesh_terms: D019943:Amino Acid Substitution; D000818:Animals; D058507:Host Specificity; D053124:Influenza A Virus, H5N1 Subtype / Q000472:pathogenicity*; D051379:Mice; D009154:Mutation / Q000235:genetics*; D009976:Orthomyxoviridae Infections / Q000235:genetics / Q000473:pathology / Q000821:virology*; D012137:Respiratory System / Q000378:metabolism / Q000473:pathology / Q000821:virology*; D014764:Viral Proteins / Q000235:genetics* / Q000378:metabolism; D014774:Virulence; D014779:Virus Replication
chemical_list: C502893:PB2 protein, influenza virus; D014764:Viral Proteins