title: Improved detection of human papillomavirus harbored in healthy skin with FAP6085/64 primers.
abstract: FAP59/64, FAP6085/6319, and CUT primer sets were designed for detecting cutaneous HPV and have been used in many clinical and epidemiology studies. The FAP6085/64 primer set was first evaluated in this study and the FAP6085/64 combination was found to be much more sensitive than all three original primer sets by using HPV plasmids as a template. To confirm further the effectiveness of the FAP6085/64 primer set in human DNA templates, 90 palmar exfoliated cell DNA samples were used to detect the cutaneous HPV by both the FAP59/64 and FAP6085/64 primer sets. The overall proportion of HPV detection in those skin samples was 77.8% (70/90) using FAP6085/64, as compared to 55.6% (50/90) using FAP59/64. The FAP6085/64 primer set was also applied in a population based study. The proportion of HPV detection was 73.96% (2076/2807) in skin samples collected from healthy individuals, and a total of 336 different PV types were found. Sixty (17.9%) of them were fully characterized HPV types, 127 (37.8%) were putative HPV types which had been described previously, 149 (44.3%) were novel putative HPV types, and two animal PVs were also detected. These results suggest that the FAP6085/64 primer set was sensitive and effective for detection of cutaneous HPV in healthy skin samples.
authors.affiliation: Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Peking University Cancer Hospital & Institute, China.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Li
authors.forename: YaQi
authors.initials: Y
authors.lastname: Pan
authors.forename: Zhongyao
authors.initials: Z
authors.lastname: Xu
authors.forename: Qiyan
authors.initials: Q
authors.lastname: Wang
authors.forename: Dong
authors.initials: D
authors.lastname: Hang
authors.forename: Na
authors.initials: N
authors.lastname: Shen
authors.forename: Mengfei
authors.initials: M
authors.lastname: Liu
authors.forename: Chanyuan
authors.initials: C
authors.lastname: Zhang
authors.forename: Amir
authors.initials: A
authors.lastname: Abliz
authors.forename: Qiuju
authors.initials: Q
authors.lastname: Deng
authors.forename: Hong
authors.initials: H
authors.lastname: Cai
authors.forename: Yang
authors.initials: Y
authors.lastname: Ke
mesh_terms: D017931:DNA Primers / Q000235:genetics*; D006801:Humans; D027383:Papillomaviridae / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D012867:Skin / Q000821:virology*; D014773:Virology / Q000379:methods*
chemical_list: D017931:DNA Primers
keywords: Cutaneous HPV; FAP cross primers; Healthy skin; Large samples