title: Distribution of PLD and FagA, B, C and D genes in Corynebacterium pseudotuberculosis isolates from sheep and goats with caseus lymphadenitis.
abstract: Caseous lymphadenits (CL) is a chronic and subclinical disease that affects goats and sheep and, consequently, causes economic losses, especially to small producers. The purpose of this study, through use of Polymerase Chain Reaction (PCR), was to verify the presence of virulence genes of phospholipase D (PLD), integral membrane protein (FagA), iron enterobactin transporter (FagB), ATP binding cytoplasmic membrane protein (FagC) and iron siderophore binding protein (FagD) in 168 isolates of C. pseudotuberculosis obtained from cases of caseous lymphadenitis in goats and sheep. FagA, FagB and PLD genes were detected in all 145 strains isolated from abscesses in superficial lymph nodes and in 23 strains isolated from viscera. The FagC gene was positive in 167 (99.40%) isolates. The FagD gene was detected in 160 (95.23%) isolates. All virulence factors analyzed were found more frequently among isolates collected in the viscera of animals with CL, indicating a multifactorial nature, as well as variations, in the invasive potential of C. pseudotuberculosis strains.
authors.affiliation: Programa de Pós-Graduação em Ciência Animal, Universidade Federal do Vale do São Francisco, Petrolina, PE, Brazil .
authors.forename: Maria da Conceição
authors.initials: Mda C
authors.lastname: Aquino de Sá
authors.forename: Gisele Veneroni
authors.initials: GV
authors.lastname: Gouveia
authors.forename: Carina da Costa
authors.initials: Cda C
authors.lastname: Krewer
authors.forename: Josir Laine Aparecida
authors.initials: JL
authors.lastname: Veschi
authors.forename: Ana Luiza
authors.initials: AL
authors.lastname: de Mattos-Guaraldi
authors.forename: Mateus Matiuzzi
authors.initials: MM
authors.lastname: da Costa
keywords: C. pseudotuberculosis; caseous lymphadenits; virulence genes