title: Angiotensinogen gene variations and LV outflow obstruction in hypertrophic cardiomyopathy.
abstract: BACKGROUND
  Variations of angiotensinogen (AGT) gene have been associated with cardiac hypertrophy. We hypothesized that AGT gene polymorphism may play a modifier role in the diversity of left ventricular outflow obstruction.
  
  
  METHODS
  The polymorphisms of the AGT gene were genotyped in 225 patients with hypertrophic cardiomyopathy (HCM) and 243 age-and sex-matched healthy controls. The effect of the A and G alleles on the expression of the reporter gene were evaluated in vitro using dual-luciferase reporter assays.
  
  
  RESULTS
  Our results showed that the frequency of the A allele was higher in patients than in controls (50.2 % vs. 35.8 %, p < 0.05). Patients carrying the AA and AG genotypes had a higher proportion of left ventricular outflow obstruction (30.1 % vs. 17.0 %, p < 0.05) and heart failure (NYHA functional class III ~ IV, 35.4 % vs. 18.8 %, p < 0.05) than those carrying the GG genotype had. After adjusted for age, sex, the thickness of the interventricular septum, family history of HCM, and sudden death, the A allele conferred a 2.4-fold risk for left ventricular outflow obstruction than the GG genotype did (adjusted OR = 2.4, 95 %CI 1.2-4.8). The G allele suppressed the expression of the reporter gene significantly compared with the A allele (p < 0.05).
  
  
  CONCLUSIONS
  AGT gene variations may be genetic modifiers for the development of HCM.
authors.affiliation: The First Geriatric Cardiology Department, Chinese Peoples' Liberation Army General Hospital, 167 Beilishi Road, 100037, Beijing, China.
authors.forename: S
authors.initials: S
authors.lastname: Wang
authors.forename: J
authors.initials: J
authors.lastname: Wang
authors.forename: Y
authors.initials: Y
authors.lastname: Zou
authors.forename: J
authors.initials: J
authors.lastname: Wang
authors.forename: H
authors.initials: H
authors.lastname: Wang
authors.forename: R
authors.initials: R
authors.lastname: Hui
mesh_terms: D000328:Adult; D000808:Angiotensinogen / Q000235:genetics*; D002312:Cardiomyopathy, Hypertrophic / Q000453:epidemiology* / Q000235:genetics*; D002681:China / Q000453:epidemiology; D015897:Comorbidity; D005260:Female; D005819:Genetic Markers / Q000235:genetics; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D014644:Genetic Variation / Q000235:genetics; D006801:Humans; D008297:Male; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D015995:Prevalence; D012307:Risk Factors; D014694:Ventricular Outflow Obstruction / Q000453:epidemiology* / Q000235:genetics*
chemical_list: D005819:Genetic Markers; D000808:Angiotensinogen