title: Identification of HSP90 as potential biomarker of biliary atresia using two-dimensional electrophoresis and mass spectrometry.
abstract: Biliary atresia (BA) is a devastating cholestatic liver disease targeting infants. Current diagnosis depends on surgical exploration of the biliary tree. The aim of the present study was to identify potential biomarkers for the diagnosis of biliary atresia (BA). Two-dimensional electrophoresis was utilized for the identification of proteins that were differentially expressed in liver biopsies of 20 BA patients and 12 infants with non-BA neonatal cholestasis (NC) as controls. Using mass spectrometry, we identified 15 proteins with expressions significantly altered. Out of the 15 proteins identified, heat shock protein (HSP) 90 was the most significantly altered and was down-regulated in BA samples compared to NC samples using immunoblotting analysis. Our findings suggest that HSP90 might be a potential biomarker for the diagnosis of BA and may be used for monitoring further development and therapy for BA. This study demonstrated that a comprehensive strategy of proteomic identification combined with further validation should be adopted in biomarker discovery.
authors.affiliation: Department of Pediatric Surgery, Children's Hospital of Fudan University, and Key Laboratory of Neonatal Disease, Ministry of Health, Shanghai, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Dong
authors.forename: Panmo
authors.initials: P
authors.lastname: Deng
authors.forename: Yanlei
authors.initials: Y
authors.lastname: Huang
authors.forename: Chun
authors.initials: C
authors.lastname: Shen
authors.forename: Ping
authors.initials: P
authors.lastname: Xue
authors.forename: Shan
authors.initials: S
authors.lastname: Zheng
mesh_terms: D001656:Biliary Atresia / Q000175:diagnosis* / Q000235:genetics / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism; D001706:Biopsy / Q000379:methods; D002779:Cholestasis / Q000175:diagnosis / Q000235:genetics / Q000378:metabolism; D015536:Down-Regulation; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D005260:Female; D018841:HSP90 Heat-Shock Proteins / Q000235:genetics* / Q000378:metabolism*; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D007232:Infant, Newborn, Diseases / Q000175:diagnosis / Q000235:genetics / Q000378:metabolism; D008099:Liver / Q000378:metabolism / Q000601:surgery; D008297:Male; D013058:Mass Spectrometry / Q000379:methods; D040901:Proteomics / Q000379:methods
chemical_list: D015415:Biomarkers; D018841:HSP90 Heat-Shock Proteins