title: Live cell imaging of RhoGTPase biosensors in tumor cells.
abstract: Tumor cell motility and invasion rely on actin cytoskeleton rearrangements mediated by the activation of RhoGTPase signaling pathways. Invadopodia are membrane-degrading protrusions that mediate extracellular matrix degradation. Here, we provide procedures for imaging RhoGTPase biosensors in tumor cells during the formation of invadopodia and matrix degradation.
authors.affiliation: Department of Anatomy and Structural Biology and Gruss Lipper Biophotonics Center, Albert Einstein College of Medicine of Yeshiva University, Bronx, NY, USA.
authors.forename: Jose Javier
authors.initials: JJ
authors.lastname: Bravo-Cordero
authors.forename: Yasmin
authors.initials: Y
authors.lastname: Moshfegh
authors.forename: John
authors.initials: J
authors.lastname: Condeelis
authors.forename: Louis
authors.initials: L
authors.lastname: Hodgson
mesh_terms: D008841:Actin Cytoskeleton; D015374:Biosensing Techniques; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics*; D022081:Cell Surface Extensions / Q000235:genetics; D005109:Extracellular Matrix / Q000378:metabolism / Q000648:ultrastructure; D006801:Humans; D008967:Molecular Biology / Q000379:methods*; D057054:Molecular Imaging / Q000379:methods*; D009369:Neoplasms / Q000473:pathology*; D020741:rho GTP-Binding Proteins / Q000737:chemistry / Q000378:metabolism*
chemical_list: D020741:rho GTP-Binding Proteins