title: Simultaneous identification of hydroxythiohomosildenafil, aminotadalafil, thiosildenafil, dimethylsildenafil, and thiodimethylsildenafil in dietary supplements using high-performance liquid chromatography-mass spectrometry.
abstract: We developed a method for the separation and identification of illegal adulterants (hydroxythiohomosildenafil, aminotadalafil, thiosildenafil, dimethylsildenafil, and thiodimethylsildenafil) from dietary supplements using high-performance liquid chromatography-mass spectrometry. The separation was achieved on a C18 column: the mobile phase consisted of 5 mM ammonium formate (pH 6.3)-acetonitrile (75 : 25, v/v) and acetonitrile, with gradient elution at a flow rate of 0.2 mL/min. The proposed method could also be used to separate vardenafil, homosildenafil, and dimethylsildenafil, all of which have the same molecular weight. Furthermore, the proposed method could simultaneously separate hydroxythiohomosildenafil, aminotadalafil, thiosildenafil, dimethylsildenafil, thiodimethylsildenafil, vardenafil, and homosildenafil. Thus, this method may be useful to identify medicinal ingredients for erectile dysfunction and their analogs and to control the quality of dietary supplements.
authors.affiliation: Osaka Prefectural Institute of Public Health, 1-3-69 Nakamichi, Higashinari-ku, Osaka 537-0025, Japan. tagami@iph.pref.osaka.jp
authors.forename: Takaomi
authors.initials: T
authors.lastname: Tagami
authors.forename: Akihiro
authors.initials: A
authors.lastname: Takeda
authors.forename: Akiko
authors.initials: A
authors.lastname: Asada
authors.forename: Airin
authors.initials: A
authors.lastname: Aoyama
authors.forename: Takahiro
authors.initials: T
authors.lastname: Doi
authors.forename: Keiji
authors.initials: K
authors.lastname: Kajimura
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Sawabe
mesh_terms: D052117:Benzodioxoles / Q000302:isolation & purification*; D002243:Carbolines / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D019587:Dietary Supplements / Q000032:analysis*; D004340:Drug Contamination; D013058:Mass Spectrometry / Q000379:methods*; D058986:Phosphodiesterase 5 Inhibitors / Q000302:isolation & purification*; D010879:Piperazines / Q000302:isolation & purification; D011687:Purines / Q000302:isolation & purification; D011743:Pyrimidines / Q000302:isolation & purification*; D000068677:Sildenafil Citrate; D013450:Sulfones / Q000302:isolation & purification*
chemical_list: D052117:Benzodioxoles; D002243:Carbolines; D058986:Phosphodiesterase 5 Inhibitors; D010879:Piperazines; D011687:Purines; D011743:Pyrimidines; D013450:Sulfones; D000068677:Sildenafil Citrate; C551065:aminotadalafil; C539492:thiosildenafil