title: Purification of native Arp2/3 complex from bovine thymus.
abstract: The Arp2/3 complex is an actin filament nucleator involved in cell motility and vesicle trafficking. Owing to the role the complex plays in important and fundamental cell biological processes, the purified complex is used in biochemical assays, reconstituted motility assays, and structural biology. As this is a eukaryotic complex assembled from seven polypeptides, the complex is purified from eukaryotic sources. Described here is a detailed method for purification of the complex from a mammalian tissue, bovine thymus.
authors.affiliation: Department of Biophysics, UT Southwestern Medical Center and Howard Hughes Medical Institute, Dallas, TX, USA.
authors.forename: Lynda K
authors.initials: LK
authors.lastname: Doolittle
authors.forename: Michael K
authors.initials: MK
authors.lastname: Rosen
authors.forename: Shae B
authors.initials: SB
authors.lastname: Padrick
mesh_terms: D008841:Actin Cytoskeleton / Q000737:chemistry / Q000378:metabolism*; D051376:Actin-Related Protein 2-3 Complex / Q000737:chemistry / Q000302:isolation & purification*; D000818:Animals; D002417:Cattle; D054443:Cell Migration Assays; D008967:Molecular Biology / Q000379:methods*; D010455:Peptides / Q000302:isolation & purification
chemical_list: D051376:Actin-Related Protein 2-3 Complex; D010455:Peptides