title: Isolation rates of Campylobacter fetus subsp venerealis from bovine preputial samples via passive filtration on nonselective medium versus selective medium, with and without transport medium.
abstract: OBJECTIVE
  To compare the recovery rates of Campylobacter fetus subsp venerealis (Cfv) from preputial scrapings of infected bulls with passive filtration on selective medium versus nonselective medium, with and without transport medium.
  
  
  METHODS
  217 preputial scrapings from 12 bulls (4 naturally and 8 artificially infected with Cfv).
  
  
  METHODS
  Preputial scrapings were collected in 2 mL of PBS solution and bacteriologically cultured directly on Skirrow medium or passively filtered through 0.65-μm filters onto blood agar, with or without 24 hour preincubation in modified Weybridge transport enrichment medium (TEM). After 72 hours, plates were examined for Cfv and bacterial and fungal contamination or overgrowth.
  
  
  RESULTS
  Passive filtration of fresh preputial scrapings onto blood agar yielded significantly higher recovery rates of Cfv (86%) than direct plating on Skirrow medium (32%), whereas recovery from TEM was poor for both media (35% and 40%, respectively). Skirrow cultures without TEM were significantly more likely to have fungal contamination than were cultures performed with any other technique, and fungal contamination was virtually eliminated by passive filtration onto blood agar. Bacterial contamination by Pseudomonas spp was significantly more common with Skirrow medium versus passive filtration on blood agar, regardless of TEM use.
  
  
  CONCLUSIONS
  The use of transport medium and the choice of culture medium had significant effects on Cfv recovery and culture contamination rates from clinical samples. Both factors should be considered when animals are tested for this pathogen.
authors.affiliation: Department of Veterinary Microbiology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, SK S7N 5B4, Canada.
authors.forename: Bonnie
authors.initials: B
authors.lastname: Chaban
authors.forename: Alvaro
authors.initials: A
authors.lastname: Garcia Guerra
authors.forename: Steven H
authors.initials: SH
authors.lastname: Hendrick
authors.forename: Cheryl L
authors.initials: CL
authors.lastname: Waldner
authors.forename: Janet E
authors.initials: JE
authors.lastname: Hill
mesh_terms: D000818:Animals; D001431:Bacteriological Techniques / Q000662:veterinary*; D002169:Campylobacter Infections / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary*; D002168:Campylobacter fetus / Q000145:classification* / Q000302:isolation & purification*; D002417:Cattle; D003470:Culture Media / Q000737:chemistry*; D005837:Genitalia, Male / Q000382:microbiology*; D008297:Male; D015231:Sexually Transmitted Diseases, Bacterial / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary; D013048:Specimen Handling / Q000379:methods / Q000662:veterinary
chemical_list: D003470:Culture Media