title: Generation of porcine fibroblasts overexpressing 11β-HSD1 with adipose tissue-specific aP2 promoter as a porcine model of metabolic syndrome.
abstract: Metabolic syndrome arises from a combination of disorders that increase the risk of cardiovascular disease and diabetes. In previous studies, it was observed that overexpression of 11β-hydroxysteroid dehydrogenase type 1 (11β-HSD1) induced obesity and the insulin resistance that accompanies metabolic syndrome in rodent adipose tissue. Based on these observations, it was hypothesized that overexpression of 11β-HSD1 may be suitable for the generation of a porcine model of metabolic syndrome. It was evaluated that promoter activities of the porcine adipose fatty acid-binding protein (aP2) gene generates adipose tissue-specific 11β-HSD1 expression. In adipose tissue, the maximum promoter activity (-2,826 to +51 nt) of aP2 was 200-fold higher than that of a promoterless construct. In addition, 11β-HSD1 transcriptional levels were significantly increased following the introduction of the aP2 promoter into 3T3‑L1 adipocytes. These observations indicate that the aP2 promoter may facilitate 11β-HSD1 overexpression in porcine adipose tissue. Transgenic fibroblasts were generated containing 11β-HSD1 cDNA controlled by the aP2 promoter with two screening markers, green fluorescence protein and a neomycin-resistance gene. It was hypothesized that transgenic fibroblasts may be useful for generating a porcine model of metabolic syndrome.
authors.affiliation: Laboratory of Veterinary Biochemistry and Molecular Biology, College of Veterinary Medicine, Chungbuk National University, Cheongju, Chungcheongbuk‑do 361‑763, Republic of Korea.
authors.forename: Eui-Man
authors.initials: EM
authors.lastname: Jung
authors.forename: Beum-Soo
authors.initials: BS
authors.lastname: An
authors.forename: Yu-Kyung
authors.initials: YK
authors.lastname: Kim
authors.forename: Young-Hee
authors.initials: YH
authors.lastname: Jeong
authors.forename: Woo-Suk
authors.initials: WS
authors.lastname: Hwang
authors.forename: Eui-Bae
authors.initials: EB
authors.lastname: Jeung
mesh_terms: D043205:11-beta-Hydroxysteroid Dehydrogenase Type 1 / Q000235:genetics / Q000378:metabolism*; D041721:3T3-L1 Cells; D000273:Adipose Tissue / Q000378:metabolism*; D000818:Animals; D004195:Disease Models, Animal; D050556:Fatty Acid-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D005347:Fibroblasts / Q000378:metabolism; D024821:Metabolic Syndrome / Q000378:metabolism / Q000473:pathology; D051379:Mice; D010957:Plasmids / Q000378:metabolism; D011401:Promoter Regions, Genetic; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D013552:Swine; D014162:Transfection
chemical_list: D050556:Fatty Acid-Binding Proteins; D011994:Recombinant Proteins; D043205:11-beta-Hydroxysteroid Dehydrogenase Type 1