title: Cyclic enzymatic amplification method (CEAM) based on exonuclease III for highly sensitive bioanalysis.
abstract: Nucleic acid molecular probes (NAMPs) have been widely used in the sensing of various chemical and biological substances, as well as physical parameters. However, for traditional nucleic acid molecular probes, the stoichiometric 1:1 binding ratio limits the signal enhancement and thus the sensitivity of the assay. In order to overcome this problem, the cyclic enzymatic amplification method (CEAM) based on exonuclease III has been applied in optical and electrical detection of DNA, proteins and small molecules with excellent sensitivity, selectivity, versatility and simplicity. In this review, the working principle of CEAM is first introduced, followed by the applications of CEAM using different output signals for various analytes. Finally, experimental designs and procedures of CEAM are discussed in detail using displacing probes-based CEAM and linear molecular beacons (LMBs)-based CEAM as two examples.
authors.affiliation: State Key Laboratory of Physical Chemistry of Solid Surfaces, The Key Laboratory for Chemical Biology of Fujian Province, Key Laboratory of Analytical Science, Department of Chemical Biology, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, PR China.
authors.forename: Liang
authors.initials: L
authors.lastname: Cui
authors.forename: Guoliang
authors.initials: G
authors.lastname: Ke
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Lin
authors.forename: Yanling
authors.initials: Y
authors.lastname: Song
authors.forename: Huimin
authors.initials: H
authors.lastname: Zhang
authors.forename: Zhichao
authors.initials: Z
authors.lastname: Guan
authors.forename: Zhi
authors.initials: Z
authors.lastname: Zhu
authors.forename: Chaoyong James
authors.initials: CJ
authors.lastname: Yang
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D005090:Exodeoxyribonucleases / Q000235:genetics; D005456:Fluorescent Dyes; D006801:Humans; D009696:Nucleic Acids / Q000302:isolation & purification*; D011506:Proteins / Q000302:isolation & purification*
chemical_list: D005456:Fluorescent Dyes; D009696:Nucleic Acids; D011506:Proteins; D005090:Exodeoxyribonucleases; C020133:exodeoxyribonuclease III
keywords: Aptamers; Molecular beacons; Molecular probes; Nuclease; Signal amplification