title: [Influence of rhizobial (Rhizobium leguminosarum) inoculation and calcium ions on the NADPH oxidase activity in roots of etiolated pea (Pisum sativum L.) seedlings].
abstract: Changes in the functional activity of the NADPH oxidase in the microsomal fraction of roots of etiolated pea seedlings, caused by rhizobial inoculation and calcium ions (Ca2+), are shown. The enzyme activity in a medium with an exogenous source of Ca2+ (CaCl2, 100 microM) fluctuated, increasing 5 to 20 min and decreasing 10 and 30 min after addition. A calcium chelator (ethylene glycol tetraacetic acid (EDTA), 100 microM) potentiated the decrease in the enzyme activity in the presence of exogenous calcium. Rhizobial inoculation caused a 3.9-fold increase in the enzyme activity 5 min after inoculation compared to the control (without inoculation). The Ca(2+)-channel activator (amiodarone, 300 microM) and the Ca(2+)-channel blocker (lanthanum chloride, 400 microM) reduced the NADPH oxidase activity after rhizobial inoculation compared to the control level (without inoculation). It is concluded that Ca2+ and reactive oxygen species are involved in the regulation of the membrane NADPH oxidase activity in roots of pea seedlings.
authors.forename: A K
authors.initials: AK
authors.lastname: Glian'ko
authors.forename: A A
authors.initials: AA
authors.lastname: Ishchenko
mesh_terms: D002118:Calcium / Q000494:pharmacology; D007477:Ions / Q000494:pharmacology; D019255:NADPH Oxidases / Q000378:metabolism*; D010084:Oxidation-Reduction; D018532:Pisum sativum / Q000201:enzymology* / Q000254:growth & development; D018517:Plant Roots / Q000201:enzymology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D016961:Rhizobium leguminosarum / Q000254:growth & development / Q000378:metabolism; D036226:Seedlings / Q000201:enzymology / Q000254:growth & development
chemical_list: D007477:Ions; D017382:Reactive Oxygen Species; D019255:NADPH Oxidases; D002118:Calcium