title: Zn- and Mg- containing tricalcium phosphates-based adjuvants for cancer immunotherapy.
abstract: Zn-, and Mg-containing tricalcium phosphates (TCPs) loaded with a hydrothermal extract of a human tubercle bacillus (HTB) were prepared by immersing Zn-TCP and Mg-TCP in HTB-containing supersaturated calcium phosphate solutions. The in vitro and in vivo immunogenic activities of the HTB-loaded Zn-, and Mg-TCPs (Zn-Ap-HTB and Mg-Ap-HTB, respectively) were evaluated as potential immunopotentiating adjuvants for cancer immunotherapy. The Zn-Ap-HTB and Mg-Ap-HTB adjuvants showed no obvious cytotoxicity and more effectively stimulated granulocyte-macrophage colony-stimulating factor (GM-CSF) secretion by macrophage-like cells than unprocessed HTB or HTB-loaded TCP (T-Ap-HTB) in vitro. Zn-Ap-HTB and Mg-Ap-HTB mixed with liquid-nitrogen-treated tumor tissue markedly inhibited the in vivo development of rechallenged Lewis lung carcinoma (LLC) cells compared with T-Ap-HTB and the unprocessed HTB mixed liquid-nitrogen-treated tumor tissue. Zn-Ap-HTB and Mg-Ap-HTB contributed to eliciting potent systemic antitumor immunity in vivo.
authors.affiliation: Human Technology Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki, Japan. xp-wang@aist.go.jp
authors.forename: Xiupeng
authors.initials: X
authors.lastname: Wang
authors.forename: Xia
authors.initials: X
authors.lastname: Li
authors.forename: Kazuo
authors.initials: K
authors.lastname: Onuma
authors.forename: Yu
authors.initials: Y
authors.lastname: Sogo
authors.forename: Tadao
authors.initials: T
authors.lastname: Ohno
authors.forename: Atsuo
authors.initials: A
authors.lastname: Ito
mesh_terms: D000276:Adjuvants, Immunologic; D000818:Animals; D001407:Bacillus / Q000737:chemistry / Q000276:immunology; D002130:Calcium Phosphates / Q000008:administration & dosage / Q000737:chemistry* / Q000276:immunology* / Q000633:toxicity; D019496:Cancer Vaccines / Q000008:administration & dosage / Q000737:chemistry / Q000276:immunology / Q000633:toxicity; D016207:Cytokines / Q000096:biosynthesis; D004195:Disease Models, Animal; D005260:Female; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000096:biosynthesis; D006801:Humans; D007167:Immunotherapy; D008264:Macrophages / Q000276:immunology / Q000378:metabolism; D008274:Magnesium / Q000737:chemistry; D051379:Mice; D009369:Neoplasms / Q000276:immunology* / Q000401:mortality / Q000628:therapy*; D013154:Spleen / Q000276:immunology / Q000378:metabolism; D018074:Vaccines, Conjugate; D015032:Zinc / Q000737:chemistry
chemical_list: D000276:Adjuvants, Immunologic; D002130:Calcium Phosphates; D019496:Cancer Vaccines; D016207:Cytokines; D018074:Vaccines, Conjugate; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D008274:Magnesium; D015032:Zinc; C018392:tricalcium phosphate