title: Construction and characterization of an infectious cDNA clone of enterovirus type 71 subgenotype C4.
abstract: Enterovirus 71 (EV71) is the major causative agent of hand, foot, and mouth disease and induces fatal neurological complications. In recent years, this virus has become a major threat to public health in the Asia-Pacific region, while no effective antiviral therapies and vaccines are currently available. In this study, we constructed and characterized for the first time an infectious full-length EV71 cDNA clone derived from the SHZH98 strain, which was the first subgenotype C4 strain isolated in China. Our data demonstrate that the rescued EV71 viruses exhibited growth kinetics in vitro and morphologies similar to those of the BrCr-TR strain and reached a maximum titer of 10(7.5) TCID50/ml. Although the rescued viruses were able to infect suckling mice, no typical symptoms of EV71 infection were observed for up to 18 days post-inoculation. Taken together our research provides an important tool to study the epidemic strains of EV71 in the Asia-Pacific region and promote the development of vaccines.
authors.affiliation: Institute of Medicinal Biotechnology, Chinese Academy of Medical Science and Peking Union Medical School, No. 1 Tian Tan Xi Li, Beijing, 100050, People's Republic of China.
authors.forename: Yong-Xin
authors.initials: YX
authors.lastname: Zhang
authors.forename: Tao
authors.initials: T
authors.lastname: Wei
authors.forename: Xiao-Yu
authors.initials: XY
authors.lastname: Li
authors.forename: Xiao
authors.initials: X
authors.lastname: Yin
authors.forename: Yu-Huan
authors.initials: YH
authors.lastname: Li
authors.forename: Ji-Wei
authors.initials: JW
authors.lastname: Ding
authors.forename: Jin-Ming
authors.initials: JM
authors.lastname: Zhou
authors.forename: Guang-Zhou
authors.initials: GZ
authors.lastname: Zhang
authors.forename: Qi
authors.initials: Q
authors.lastname: Jin
authors.forename: Shan
authors.initials: S
authors.lastname: Cen
mesh_terms: D000818:Animals; D002681:China; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics*; D004195:Disease Models, Animal; D029821:Enterovirus A, Human / Q000235:genetics* / Q000302:isolation & purification / Q000502:physiology*; D005838:Genotype; D006232:Hand, Foot and Mouth Disease / Q000473:pathology / Q000821:virology; D051379:Mice; D008969:Molecular Sequence Data; D012367:RNA, Viral / Q000235:genetics*; D017422:Sequence Analysis, DNA; D019562:Viral Load; D014776:Virus Cultivation; D014779:Virus Replication
chemical_list: D018076:DNA, Complementary; D012367:RNA, Viral