title: Anthocyanins from Vitis coignetiae Pulliat Inhibit Cancer Invasion and Epithelial-Mesenchymal Transition, but These Effects Can Be Attenuated by Tumor Necrosis Factor in Human Uterine Cervical Cancer HeLa Cells.
abstract: Recently we have demonstrated that anthocyanins from fruits of Vitis coignetiae Pulliat (AIMs) have anticancer effects. Here, we investigate the effects of AIMs on cell proliferation and invasion as well as epithelial-mesenchymal transition (EMT) which have been linked to cancer metastasis in human uterine cervical cancer HeLa cells. AIMs inhibited the invasion of HeLa cells in a dose-dependent manner. AIMs inhibited MMP-9 expression in a dose-dependent manner. AIMs inhibited the motility of HeLa cells in a wound healing test. AIMs still suppressed NF- κ B activation induced by TNF. AIMs also inhibited EMT in HeLa cells. AIMs suppressed vimentin, N-cadherin, and β-catenin expression and induced E-cadherin. AIMs also suppressed expression of β-catenin and Snail, which was regulated by GSK-3. These effects of AIMs were also limited in the HeLa cells treated with TNF. In conclusion, this study indicates that AIMs have anticancer effects by suppressing NF- κ B-regulated genes and EMT, which relates to suppression of I κ B α phosphorylation and GSK-3 activity, respectively. However, the effects of AIMs were attenuated in the TNF-high condition.
authors.affiliation: Department of Internal Medicine, Institute of Health Sciences, Gyeongsang National University School of Medicine, 90 Chilam-dong, Jinju 660-702, Republic of Korea ; The Fifth Hospital of Shijizhuang, Shijizhuang 050021, China.
authors.forename: Jing Nan
authors.initials: JN
authors.lastname: Lu
authors.forename: Won Sup
authors.initials: WS
authors.lastname: Lee
authors.forename: Jeong Won
authors.initials: JW
authors.lastname: Yun
authors.forename: Min Jeong
authors.initials: MJ
authors.lastname: Kim
authors.forename: Hye Jung
authors.initials: HJ
authors.lastname: Kim
authors.forename: Dong Chul
authors.initials: DC
authors.lastname: Kim
authors.forename: Jae-Hoon
authors.initials: JH
authors.lastname: Jeong
authors.forename: Yung Hyun
authors.initials: YH
authors.lastname: Choi
authors.forename: Gon-Sup
authors.initials: GS
authors.lastname: Kim
authors.forename: Chung Ho
authors.initials: CH
authors.lastname: Ryu
authors.forename: Sung Chul
authors.initials: SC
authors.lastname: Shin