title: Potential use of synthetic α-galactosyl-containing glycotopes of the parasite Trypanosoma cruzi as diagnostic antigens for Chagas disease.
abstract: A synthetic glycoarray containing non-reducing α-galactopyranosyl moieties related to mucin O-glycans of the parasite Trypanosoma cruzi was evaluated by a chemiluminescent enzyme-linked immunosorbent assay with sera from patients with chronic Chagas disease. Our data revealed the disaccharide Galα(1,3)Galβ as the immunodominant glycotope, which may eventually be employed as a diagnostic antigen for Chagas disease.
authors.affiliation: Department of Chemistry, University of Texas at El Paso, El Paso, TX 79968, USA.
authors.forename: Roger A
authors.initials: RA
authors.lastname: Ashmus
authors.forename: Nathaniel S
authors.initials: NS
authors.lastname: Schocker
authors.forename: Yanira
authors.initials: Y
authors.lastname: Cordero-Mendoza
authors.forename: Alexandre F
authors.initials: AF
authors.lastname: Marques
authors.forename: Erika Y
authors.initials: EY
authors.lastname: Monroy
authors.forename: Andrew
authors.initials: A
authors.lastname: Pardo
authors.forename: Luis
authors.initials: L
authors.lastname: Izquierdo
authors.forename: Montserrat
authors.initials: M
authors.lastname: Gállego
authors.forename: Joaquim
authors.initials: J
authors.lastname: Gascon
authors.forename: Igor C
authors.initials: IC
authors.lastname: Almeida
authors.forename: Katja
authors.initials: K
authors.lastname: Michael
mesh_terms: D000937:Antigen-Antibody Reactions; D002236:Carbohydrate Conformation; D014355:Chagas Disease / Q000175:diagnosis* / Q000276:immunology / Q000382:microbiology; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000737:chemistry* / Q000276:immunology; D005690:Galactose / Q000737:chemistry* / Q000276:immunology; D006801:Humans; D014349:Trypanosoma cruzi / Q000737:chemistry* / Q000276:immunology
chemical_list: D000939:Epitopes; D005690:Galactose