title: Human antibody neutralizes severe Fever with thrombocytopenia syndrome virus, an emerging hemorrhagic Fever virus.
abstract: Severe fever with thrombocytopenia syndrome virus (SFTSV), a newly discovered member of the Bunyaviridae family, is the causative agent of an emerging hemorrhagic fever, SFTS, in China. Currently, there are no vaccines or effective therapies against SFTS. In this study, a combinatorial human antibody library was constructed from the peripheral lymphocytes of 5 patients who had recovered from SFTS. The library was screened against purified virions for the production of single-chain variable-region fragments (ScFv). Of the 6 positive clones, one clone (monoclonal antibody [MAb] 4-5) showed neutralizing activity against SFTSV infection in Vero cells. MAb 4-5 was found to effectively neutralize all of the clinical isolates of SFTSV tested, which were isolated from patients in China from 2010 to 2012. MAb 4-5 was found to bind a linear epitope in the ectodomain of glycoprotein Gn. Its neutralizing activity is attributed to blockage of the interactions between the Gn protein and the cellular receptor, indicating that inhibition of virus-cell attachment is its main mechanism. These data suggest that MAb 4-5 can be used as a promising candidate molecule for immunotherapy against SFTSV infection.
authors.affiliation: Institute of Pathogenic Microbiology, Jiangsu Provincial Center for Disease Prevention and Control, Key Laboratory of Enteric Pathogenic Microbiology, Ministry Health, Nanjing, China.
authors.forename: Xiling
authors.initials: X
authors.lastname: Guo
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.forename: Wenshuai
authors.initials: W
authors.lastname: Zhang
authors.forename: Ying
authors.initials: Y
authors.lastname: Chi
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Zeng
authors.forename: Xian
authors.initials: X
authors.lastname: Li
authors.forename: Xian
authors.initials: X
authors.lastname: Qi
authors.forename: Qiu
authors.initials: Q
authors.lastname: Jin
authors.forename: Xiao
authors.initials: X
authors.lastname: Zhang
authors.forename: Mingming
authors.initials: M
authors.lastname: Huang
authors.forename: Hua
authors.initials: H
authors.lastname: Wang
authors.forename: Yin
authors.initials: Y
authors.lastname: Chen
authors.forename: Changjun
authors.initials: C
authors.lastname: Bao
authors.forename: Jianli
authors.initials: J
authors.lastname: Hu
authors.forename: Shuyi
authors.initials: S
authors.lastname: Liang
authors.forename: Lin
authors.initials: L
authors.lastname: Bao
authors.forename: Tao
authors.initials: T
authors.lastname: Wu
authors.forename: Minghao
authors.initials: M
authors.lastname: Zhou
authors.forename: Yongjun
authors.initials: Y
authors.lastname: Jiao
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000302:isolation & purification; D057134:Antibodies, Neutralizing / Q000276:immunology* / Q000302:isolation & purification; D000914:Antibodies, Viral / Q000276:immunology* / Q000302:isolation & purification; D002043:Bunyaviridae / Q000276:immunology*; D002681:China; D002522:Chlorocebus aethiops; D021821:Communicable Diseases, Emerging / Q000276:immunology*; D018604:Epitope Mapping; D006482:Hemorrhagic Fevers, Viral / Q000276:immunology*; D006801:Humans; D009500:Neutralization Tests; D014709:Vero Cells; D053585:Virus Attachment / Q000187:drug effects
chemical_list: D000911:Antibodies, Monoclonal; D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral