title: Overexpression of cyclooxygenase-1 correlates with poor prognosis in renal cell carcinoma.
abstract: The aim of this study was to evaluate expression of COX-1 in renal cell carcinoma (RCC) and its prognostic value. mRNA of COX-1 was detected in 42 paired RCC and adjacent normal tissues with quantitative real- time polymerase chain reaction (qRT-PCR). Expression of COX-1 was also evaluated in 196 RCC sections and 91 adjacent normal tissues with immunohistochemistry. Statistical analysis was performed to assess COX-1 expression in RCC and its prognostic significance. The results of qRT-PCR showed mRNA levels of COX-1 in RCC tissues to be significantly higher than that in adjacent normal tissues (p < 0.001). Immunohistochemical assays also revealed COX-1 to be overexpressed in RCC tissues (p < 0.001). Statistical analysis demonstrated high expression of COX-1 was correlated with tumour size (p = 0.002), pathological stage (p = 0.003), TNM stage (p = 0.003, 0.007, 0.027, respectively), and tumour recurrence (p < 0.001). Survival analysis indicated patients with high expression of COX-1 had shorter survival time (p < 0.001), and COX-1 was an independent predictor. This is the first study to reveal overexpression of COX-1 in RRC and point to use as a prognostic marker in affected patients.
authors.affiliation: Department of Urology, Peking University Shenzhen Hospital, Shenzhen, China.
authors.forename: Zu-Hu
authors.initials: ZH
authors.lastname: Yu
authors.forename: Qiang
authors.initials: Q
authors.lastname: Zhang
authors.forename: Ya-Dong
authors.initials: YD
authors.lastname: Wang
authors.forename: Jing
authors.initials: J
authors.lastname: Chen
authors.forename: Zhi-Mao
authors.initials: ZM
authors.lastname: Jiang
authors.forename: Min
authors.initials: M
authors.lastname: Shi
authors.forename: Xin
authors.initials: X
authors.lastname: Guo
authors.forename: Jie
authors.initials: J
authors.lastname: Qin
authors.forename: Guang-Hui
authors.initials: GH
authors.lastname: Cui
authors.forename: Zhi-Ming
authors.initials: ZM
authors.lastname: Cai
authors.forename: Yao-Ting
authors.initials: YT
authors.lastname: Gui
authors.forename: Yong-Qing
authors.initials: YQ
authors.lastname: Lai
mesh_terms: D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism*; D002291:Carcinoma, Papillary / Q000378:metabolism / Q000401:mortality* / Q000556:secondary; D002292:Carcinoma, Renal Cell / Q000378:metabolism / Q000401:mortality* / Q000556:secondary; D051545:Cyclooxygenase 1 / Q000235:genetics / Q000378:metabolism*; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D007124:Immunoenzyme Techniques; D007668:Kidney / Q000378:metabolism / Q000473:pathology; D007680:Kidney Neoplasms / Q000378:metabolism / Q000401:mortality* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009362:Neoplasm Metastasis; D009364:Neoplasm Recurrence, Local / Q000378:metabolism / Q000401:mortality* / Q000473:pathology; D009367:Neoplasm Staging; D011379:Prognosis; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015996:Survival Rate
chemical_list: D014408:Biomarkers, Tumor; D012333:RNA, Messenger; D051545:Cyclooxygenase 1; C496511:PTGS1 protein, human