title: Long term behavior of biological prostheses used as abdominal wall substitutes.
abstract: Despite their degradation in the host organism, the benefits of collagen bioprostheses remain unclear. This study addresses the absorption and long-term host tissue incorporation of several collagen biomeshes.
  
  
  
  Partial ventral hernial defects created in the abdominal wall of rabbits were repaired using the crosslinked meshes Permacol® or CollaMend®, or the non-crosslinked Surgisis®, Tutomesh® or Strattice®. After 90 and 180 days of implant, morphological studies and morphometric analysis of the thickness of the meshes were performed. Immunofluorescence confocal microscopy combined with differential interference contrast (DIC) imaging was used to distinguish newly formed collagen from that comprising the mesh. The macrophage response was examined by immunohistochemistry.
  
  
  
  At 90 days, the thinner non-crosslinked biomeshes Tutomesh and Surgisis were more fully degraded with much of their collagen replaced with loose connective tissue. By 180 days, both implants had been practically fully absorbed. In contrast, in Strattice only the outermost third was infiltrated by neoformed tissue. On both surfaces of the crosslinked meshes, a fibrous capsule with host cells lining its perimeter was observed at both time points, though at 180 days these cells had penetrated the mesh interior. At both implant times, Strattice showed the higher expression of collagen type I while collagen III expression was similar for all the meshes. The non-crosslinked materials elicited lower macrophage counts at both time points, significantly so for Strattice. The macrophage response decreased over time for all the meshes but Surgisis.
  
  
  
  Strattice, the thicker, more compacted non-crosslinked mesh showed the best balance between tissue incorporation and absorption while eliciting a minimal foreign-body reaction in the long-term.
authors.affiliation: Department of Medical Specialities, Networking Research Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN). Faculty of Medicine, Alcalá University, Madrid, Spain, gemma.pascual@uah.es.
authors.forename: G
authors.initials: G
authors.lastname: Pascual
authors.affiliation: Department of Medical Specialities, Networking Research Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN). Faculty of Medicine, Alcalá University, Madrid, Spain.
authors.forename: S
authors.initials: S
authors.lastname: Sotomayor
authors.affiliation: Department of Surgery, Networking Research Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN). Faculty of Medicine, Alcalá University, Madrid, Spain.
authors.forename: P
authors.initials: P
authors.lastname: Pérez-López
authors.affiliation: Department of Medical Specialities, Networking Research Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN). Faculty of Medicine, Alcalá University, Madrid, Spain.
authors.forename: J
authors.initials: J
authors.lastname: Buján
authors.affiliation: Department of Surgery, Networking Research Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN). Faculty of Medicine, Alcalá University, Madrid, Spain.
authors.forename: J M
authors.initials: JM
authors.lastname: Bellón
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000009:adverse effects*; D001705:Bioprosthesis / Q000009:adverse effects*; D003094:Collagen / Q000627:therapeutic use*; D006555:Hernia, Ventral / Q000601:surgery*; D059685:Herniorrhaphy / Q000295:instrumentation*; D011817:Rabbits; D013526:Surgical Mesh / Q000009:adverse effects*
chemical_list: D001672:Biocompatible Materials; D003094:Collagen