title: Cysteamine-palladium complex ([Pd(μ-OAc)(ppy)]2, ppy:2-phenylpyridine, PhMe)-modified peroxidase biosensor immobilized on a gold electrode.
abstract: A new peroxidase biosensor was developed using cysteamine-palladium complex-modified gold electrode. The principle of the measurements is based on monitoring increase in the oxidation potential of palladium complex (at + 0.47 V vs Ag/AgCl) using amperometric detection. In the optimization studies of the biosensor, effects of enzyme amount, palladium complex amount, and duration of SAM formation on biosensor responses were investigated to optimize the bioactive layer. The biosensor has a fast response time of less than 10 s to hydrogen peroxide (H2O2), with a linear range of 5.0 × 10(- 6) to 150 × 10(- 6) M and a detection limit of 3.38 × 10(- 6) M.
authors.affiliation: Faculty of Science, Department of Biochemistry, Ege University Bornova , Izmir , Turkey.
authors.forename: Erol
authors.initials: E
authors.lastname: Akyilmaz
authors.forename: M Emin
authors.initials: ME
authors.lastname: Günay
authors.forename: Engin
authors.initials: E
authors.lastname: Asav
authors.forename: Rukiye
authors.initials: R
authors.lastname: Gümüşada
mesh_terms: D015374:Biosensing Techniques; D056831:Coordination Complexes / Q000737:chemistry*; D003543:Cysteamine / Q000737:chemistry*; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000737:chemistry*; D006046:Gold / Q000737:chemistry; D006801:Humans; D006861:Hydrogen Peroxide / Q000737:chemistry; D010084:Oxidation-Reduction; D009195:Peroxidase / Q000737:chemistry*
chemical_list: D056831:Coordination Complexes; D004800:Enzymes, Immobilized; D003543:Cysteamine; D006046:Gold; D006861:Hydrogen Peroxide; D009195:Peroxidase
keywords: biosensor; cysteamine–palladium complex; hydrogen peroxide; peroxidase