title: Aptamer-linked biosensor for thrombin based on AuNPs/thionine-graphene nanocomposite.
abstract: A novel protocol of electrochemical thrombin aptasensor based on a goldnanoparticles/thionine-graphene (AuNPs/Th-G) nanocomposite modified glassy carbon electrode was presented. Graphene was non-covalently functionalized by thionine viaπ-stacking interaction, and then AuNPs were electrodeposited onto the Th-G surface. The morphology and conductivity of the AuNPs/Th-G nanocomposite were characterized by scanning electron microscopy, cyclic voltammetry and electrochemical impedance spectroscopy. The ethanethiol-substituted oligonucleotide probe was immobilized onto the surface of the AuNPs/Th-G nanocomposite to prepare an electrochemical biosensing platform. The aptamer-thrombin reaction was monitored by differential pulse voltammetry. Under optimum conditions, the proposed biosensor exhibits high sensitivity and a low detection limit for thrombin determination. The thrombin could be quantified in a wide range of 0.5 to 40 nM and with a low detection limit of 0.093 nM (S/N = 3).
authors.affiliation: College of Sciences, Shanghai University, Shanghai 200444, PR China.
authors.forename: Zhao
authors.initials: Z
authors.lastname: Zhang
authors.forename: Liqiang
authors.initials: L
authors.lastname: Luo
authors.forename: Limei
authors.initials: L
authors.lastname: Zhu
authors.forename: Yaping
authors.initials: Y
authors.lastname: Ding
authors.forename: Dongmei
authors.initials: D
authors.lastname: Deng
authors.forename: Zhenxin
authors.initials: Z
authors.lastname: Wang
mesh_terms: D052157:Aptamers, Nucleotide / Q000378:metabolism*; D015374:Biosensing Techniques / Q000379:methods*; D001774:Blood Chemical Analysis; D004563:Electrochemistry; D004566:Electrodes; D006046:Gold / Q000737:chemistry*; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry*; D053761:Nanocomposites / Q000737:chemistry; D010640:Phenothiazines / Q000737:chemistry*; D015203:Reproducibility of Results; D013438:Sulfhydryl Compounds / Q000737:chemistry; D013917:Thrombin / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism
chemical_list: D052157:Aptamers, Nucleotide; D010640:Phenothiazines; D013438:Sulfhydryl Compounds; D006046:Gold; D006108:Graphite; D013917:Thrombin; C009469:thionine