title: Mode of virus rescue determines the acquisition of VHS mutations in VP22-negative herpes simplex virus 1.
abstract: It has been proposed that herpes simplex virus 1 with VP22 deleted requires secondary mutation of VHS for viability. Here we show that a replication-competent Δ22 virus constructed by homologous recombination maintains a wild-type (Wt) VHS gene and has no other gross mutations. By contrast, Δ22 viruses recovered from a bacterial artificial chromosome contain multiple amino acid changes within a conserved region of VHS. Hence, the mode of virus rescue influences the acquisition of secondary mutations.
authors.affiliation: Section of Virology, Faculty of Medicine, Imperial College London, London, United Kingdom.
authors.forename: Katja
authors.initials: K
authors.lastname: Ebert
authors.forename: Daniel P
authors.initials: DP
authors.lastname: Depledge
authors.forename: Judith
authors.initials: J
authors.lastname: Breuer
authors.forename: Laura
authors.initials: L
authors.lastname: Harman
authors.forename: Gillian
authors.initials: G
authors.lastname: Elliott
mesh_terms: D000818:Animals; D002522:Chlorocebus aethiops; D022202:Chromosomes, Artificial, Bacterial; D017353:Gene Deletion; D018259:Herpesvirus 1, Human / Q000235:genetics* / Q000254:growth & development; D059765:Homologous Recombination; D009154:Mutation; D012260:Ribonucleases / Q000235:genetics*; D014709:Vero Cells; D014764:Viral Proteins / Q000235:genetics*; D015678:Viral Structural Proteins / Q000235:genetics
chemical_list: D014764:Viral Proteins; D015678:Viral Structural Proteins; C073413:herpes simplex virus type 1 protein VP22; C070722:virion host shutoff protein, Simplexvirus; D012260:Ribonucleases