title: Secretory phospholipase A2 activity in serum and cerebrospinal fluid of patients with relapsing-remitting multiple sclerosis.
abstract: An increasing body of evidence suggests that patients afflicted with multiple sclerosis (MS) show alterations in immunologic markers including increases in proinflammatory cytokine activity and inflammation. Secretory Phospholipase A2 (sPLA2) is one of the key molecules contributing to the production of inflammatory lipid mediators, mainly eicosanoids. They are considered proinflammatory enzymes and their inhibition has long been recognized as a desirable therapeutic target. The aim of this study was to measure the enzyme activity of sPLA2 both in serum and cerebrospinal fluid (CSF) of MS patients. Twenty MS patients accompanied with 20 age-sex matched controls were recruited. The results showed that the enzyme activity of serum sPLA2 was 0.007±0.021 (μmol/min/ml) in MS patients vs. 0.007±0.016 (μmol/min/ml) in patients with other neurological diseases as a control group (P=0.5). Our findings also indicated that there is no correlation (P=0.6) between CSF sPLA2 enzyme activity and MS disease when the results of two groups were compared (0.072±0.020 in cases vs. 0.071±0.01 in control group). The results suggest that the enzyme activity of sPLA2 is not altered during the disease course.
authors.affiliation: Neurology Department, Imam Khomeini Hospital, Iranian Center of Neurological Research, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Bahaadin
authors.initials: B
authors.lastname: Siroos
authors.forename: Mohammad
authors.initials: M
authors.lastname: Balood
authors.forename: Hamid
authors.initials: H
authors.lastname: Zahednasab
authors.forename: Seyed Alireza
authors.initials: SA
authors.lastname: Mesbah-Namin
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Pourgholy
authors.forename: Mohammad Hossein
authors.initials: MH
authors.lastname: Harirchian
mesh_terms: D000328:Adult; D004789:Enzyme Activation / Q000502:physiology; D005260:Female; D006801:Humans; D008297:Male; D020529:Multiple Sclerosis, Relapsing-Remitting / Q000097:blood / Q000134:cerebrospinal fluid / Q000201:enzymology*; D054497:Phospholipases A2, Secretory / Q000378:metabolism*; D055815:Young Adult
chemical_list: D054497:Phospholipases A2, Secretory
keywords: Enzyme activity; Inflammation; Multiple sclerosis; Secretory Phospholipase A2