title: [Effects of platelets on the adhesion between leukocytes and liver sinusoidal endothelium cells as well as on the transendothelial migration of leukocytes undergoing hypoxia-reoxygenation].
abstract: OBJECTIVE
  To investigate the effects of platelet on intercellular adhesion between leukocyte and liver sinusoidal endothelial cell(LSEC) and the transendothelial migration under the hypoxia-reoxygenation condition, as well as the role of relevant adhesion molecules.
  
  
  METHODS
  LSEC was cultured for 24 hours under hypoxia condition and then reoxygenated for 2 hours (hypoxia-reoxygenation, HR). This hypoxia-reoxygenation model was used to simulate the clinical liver ischemia-reperfusion injury process (IRI). Platelets and leukocytes were labeled with fluorescence dye, and then the adhesion was detected by fluorescence microscope, fluorescence plate reader and laser scanning confocal microscope. Antibody blockage experiment was used to analyze the relevant adhesion molecules.
  
  
  RESULTS
  The adhesion between platelets and LSEC was increased significantly after HR. The fluorescence intensity of adherent platelets increased from 142.10 ± 7.53 to 289.17 ± 20.00(P < 0.01). After H-R treatment and the addition of platelets, the number of adherent leukocytes increased markedly, and a significant statistical difference (360.71 ± 23.47 and 186.39 ± 17.96, P < 0.01) was found in comparing with the platelet deficient group. These adhesion processes could be blocked respectively by anti-GPIb, anti-GPIIb, anti-GPIIIa, anti-P-selectin, anti-CD31, anti-ICAM-1, anti-VCAM-1 and anti-ELAM-1. Confocal microscopy showed that platelets located between leukocytes and LSEC, and mediated their adhesion process. However, the adhesion of platelets to LSEC decreased the transendothelial migration of leukocytes (227.79 ± 16.51 and 167.27 ± 10.92, P < 0.05).
  
  
  CONCLUSIONS
  During ischemia-reperfusion condition platelets adhere to the surface of LSEC, and then further mediate more adhesion processes between leukocytes and endothelial cells, as well as inhibit the transendothelial migration of leukocytes. The consequence is that large numbers of leukocytes were sequestrated within hepatic sinus, and deteriorate liver ischemia-reperfusion injury.
authors.affiliation: Graduate School of Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100730, China.
authors.forename: Yan-ting
authors.initials: YT
authors.lastname: Cao
authors.forename: Jin-ning
authors.initials: JN
authors.lastname: Lou
authors.forename: Wen-jian
authors.initials: WJ
authors.lastname: Zhang
authors.forename: Li-ya
authors.initials: LY
authors.lastname: Ye
authors.forename: Hong-lin
authors.initials: HL
authors.lastname: Liu
authors.forename: Cheng-hui
authors.initials: CH
authors.lastname: Li
mesh_terms: D001792:Blood Platelets / Q000166:cytology*; D002448:Cell Adhesion; D015687:Cell Hypoxia; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000166:cytology*; D004730:Endothelium, Vascular / Q000166:cytology; D006503:Hepatic Veins / Q000166:cytology; D006801:Humans; D007962:Leukocytes / Q000166:cytology*; D010100:Oxygen / Q000378:metabolism; D015427:Reperfusion Injury; D057705:Transendothelial and Transepithelial Migration
chemical_list: D010100:Oxygen