title: Mechanical, biological and structural characterization of in vitro ruptured human carotid plaque tissue.
abstract: Recent experimental studies performed on human carotid plaques have focused on mechanical characterization for the purpose of developing material models for finite-element analysis without quantifying the tissue composition or relating mechanical behaviour to preoperative classification. This study characterizes the mechanical and biological properties of 25 human carotid plaques and also investigates the common features that lead to plaque rupture during mechanical testing by performing circumferential uniaxial tests, Fourier transform infrared (FTIR) and scanning electron microscopy (SEM) on each specimen to relate plaque composition to mechanical behaviour. Mechanical results revealed large variations between plaque specimen behaviour with no correlation to preoperative ultrasound prediction. However, FTIR classification demonstrated a statistically significant relationship between stress and stretch values at rupture and the level of calcification (P=0.002 and P=0.009). Energy-dispersive X-ray spectroscopy was carried out to confirm that the calcium levels observed using FTIR analysis were accurate. This work demonstrates the potential of FTIR as an alternative method to ultrasound forpredicting plaque mechanical behaviour. SEM imaging at the rupture sites of each specimen highlighted voids created by the nodes of calcifications in the tissue structure which could lead to increased vulnerability of the plaque.
authors.affiliation: Centre for Applied Biomedical Engineering Research, Department of Mechanical, Aeronautical, and Biomedical Engineering and Material and Surface Science Institute, University of Limerick, Limerick, Ireland.
authors.forename: J J
authors.initials: JJ
authors.lastname: Mulvihill
authors.forename: E M
authors.initials: EM
authors.lastname: Cunnane
authors.forename: S M
authors.initials: SM
authors.lastname: McHugh
authors.forename: E G
authors.initials: EG
authors.lastname: Kavanagh
authors.forename: S R
authors.initials: SR
authors.lastname: Walsh
authors.forename: M T
authors.initials: MT
authors.lastname: Walsh
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D001696:Biomechanical Phenomena; D016893:Carotid Stenosis / Q000000981:diagnostic imaging / Q000473:pathology* / Q000503:physiopathology* / Q000601:surgery; D004691:Endarterectomy; D004730:Endothelium, Vascular / Q000473:pathology / Q000503:physiopathology / Q000648:ultrastructure; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D012421:Rupture; D013052:Spectrometry, X-Ray Emission; D017550:Spectroscopy, Fourier Transform Infrared; D013314:Stress, Mechanical; D014463:Ultrasonography
keywords: Atherosclerosis; Carotid; FTIR; Microscopy; Tissue characterisation