title: Assessment of genotoxicity and cytotoxicity of standardized aqueous extract from leaves of Erythroxylum cuneatum in human HepG2 and WRL68 cells line.
abstract: OBJECTIVE
  To investigate the cytotoxicity and the genotoxicity of standardized aqueous of dry leaves of Erythroxylum cuneatum (E. cuneatum) in human HepG2 and WRL68 cells.
  
  
  METHODS
  The cytotoxicity of E. cuneatum extract was evaluated by both MTS and LDH assays. Genotoxicity study on E. cuneatum extract was assessed by the single cell gel electrophoresis (comet assay). The protective effect of E. cuneatum against menadione-induced cytotoxicity was also investigated.
  
  
  RESULTS
  Results from this study showed that E. cuneatum extract exhibited cytotoxic activities towards the cells with IC50 value of (125±12) and (125±14) μg/mL for HepG2 and WRL68 cells respectively, after 72 h incubation period as determined by MTS assay. LDH leakage was detected at (251±19) and (199.5±12.0) μg/mL for HepG2 and WRL68 respectively. Genotoxicity study results showed that treatment with E. cuneatum up to 1 mg/mL did not cause obvious DNA damage in WRL68 and HepG2 cells. Addition of E. cunaetum did not show significant protection towards menadione in WRL68 and HepG2 Cells.
  
  
  CONCLUSIONS
  E. cuneatum standardized aqueous extract might be developed in order to establish new pharmacological possibilities for its application.
authors.affiliation: Faculty of Pharmacy, Universiti Teknologi MARA, Shah Alam, Selangor.
authors.forename: R K
authors.initials: RK
authors.lastname: Wesam
authors.forename: A N
authors.initials: AN
authors.lastname: Ghanya
authors.forename: H H
authors.initials: HH
authors.lastname: Mizaton
authors.forename: M
authors.initials: M
authors.lastname: Ilham
authors.forename: A
authors.initials: A
authors.lastname: Aishah
mesh_terms: D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D020552:Comet Assay; D003603:Cytotoxins / Q000633:toxicity*; D004249:DNA Damage / Q000187:drug effects; D030921:Erythroxylaceae / Q000737:chemistry*; D056945:Hep G2 Cells; D006801:Humans; D009153:Mutagens / Q000633:toxicity*; D010936:Plant Extracts / Q000633:toxicity*; D018515:Plant Leaves / Q000737:chemistry*
chemical_list: D003603:Cytotoxins; D009153:Mutagens; D010936:Plant Extracts
keywords: Cytotoxicity; DNA damage; Erythroxylum cuneatu; Genotoxicity; HepG2; WRL68