title: Decreased expression of microRNA-625 is associated with tumor metastasis and poor prognosis in patients with colorectal cancer.
abstract: BACKGROUND
  MicroRNAs (miRNAs) play an essential role in colorectal cancer (CRC) development and progression. Aberrant miR-625 expression has been reported in several cancers. However, the clinical significance of miR-625 in human CRC has not been addressed.
  
  
  METHODS
  miR-625 expression was determined in 96 pairs of primary CRC and their corresponding adjacent nontumor tissues by quantitative reverse transcriptase-polymerase chain reaction. Associations of miR-625 expression with demographic and clinicopathologic features were determined. Additionally, the effects of ectopic expression of miR-625 on cell migration and invasion were investigated in vitro and in vivo.
  
  
  RESULTS
  miR-625 was significantly downregulated in CRC tissues and cell lines. In addition, the decreased expression of miR-625 was positively associated with advanced lymph node metastasis (P = 0.038), liver metastasis (P = 0.031), poor overall survival (P = 0.002), and an unfavorable prognosis for CRC patients, as determined through a multivariate analysis (P = 0.034). Moreover, functional assays demonstrated that ectopic miR-625 expression inhibits the invasion and migration of HCT116 CRC cells both in vitro and in vivo.
  
  
  CONCLUSIONS
  Our results suggest that miR-625 may serve as an efficient clinical biomarker and a therapeutic tool for the inhibition of metastasis in CRC.
authors.affiliation: Department of General Surgery, The Ninth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, China. xlou_lou@live.com
authors.forename: Xiaolou
authors.initials: X
authors.lastname: Lou
authors.forename: Xiaoliang
authors.initials: X
authors.lastname: Qi
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhang
authors.forename: Houdong
authors.initials: H
authors.lastname: Long
authors.forename: Jianjun
authors.initials: J
authors.lastname: Yang
mesh_terms: D000818:Animals; D002465:Cell Movement; D015179:Colorectal Neoplasms / Q000235:genetics / Q000401:mortality / Q000473:pathology*; D015972:Gene Expression Regulation, Neoplastic; D045325:HCT116 Cells; D006801:Humans; D008113:Liver Neoplasms / Q000556:secondary; D008207:Lymphatic Metastasis; D051379:Mice; D035683:MicroRNAs / Q000032:analysis / Q000502:physiology*; D009361:Neoplasm Invasiveness; D011379:Prognosis; D016016:Proportional Hazards Models; D015996:Survival Rate
chemical_list: C575476:MIRN625 microRNA, human; D035683:MicroRNAs
keywords: clinicopathology; colorectal cancer; metastasis; miR-625; prognosis