title: The UL24 protein of herpes simplex virus 1 affects the sub-cellular distribution of viral glycoproteins involved in fusion.
abstract: Mutations in UL24 of herpes simplex virus type 1 can lead to a syncytial phenotype. We hypothesized that UL24 affects the sub-cellular distribution of viral glycoproteins involved in fusion. In non-immortalized human foreskin fibroblasts (HFFs) we detected viral glycoproteins B (gB), gD, gH and gL present in extended blotches throughout the cytoplasm with limited nuclear membrane staining; however, in HFFs infected with a UL24-deficient virus (UL24X), staining for the viral glycoproteins appeared as long, thin streaks running across the cell. Interestingly, there was a decrease in co-localized staining of gB and gD with F-actin at late times in UL24X-infected HFFs. Treatment with chemical agents that perturbed the actin cytoskeleton hindered the formation of UL24X-induced syncytia in these cells. These data support a model whereby the UL24 syncytial phenotype results from a mislocalization of viral glycoproteins late in infection.
authors.affiliation: INRS-Institut Armand-Frappier, Université INRS, 531 boul. des Prairies, Laval, QC, Canada H7V 1B7.
authors.forename: Nawel
authors.initials: N
authors.lastname: Ben Abdeljelil
authors.forename: Pierre-Alexandre
authors.initials: PA
authors.lastname: Rochette
authors.forename: Angela
authors.initials: A
authors.lastname: Pearson
mesh_terms: D002459:Cell Fusion; D002478:Cells, Cultured; D005347:Fibroblasts / Q000821:virology; D015726:Giant Cells / Q000166:cytology / Q000821:virology; D006023:Glycoproteins / Q000378:metabolism*; D018259:Herpesvirus 1, Human / Q000235:genetics / Q000502:physiology; D006801:Humans; D050505:Mutant Proteins / Q000235:genetics / Q000378:metabolism; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D006023:Glycoproteins; D050505:Mutant Proteins; C086267:UL24 protein, Human herpesvirus 1; D014764:Viral Proteins
keywords: F-actin; Herpes simplex virus 1; Syncytia; UL24; gB; gD