title: Ultrasound-mediated vascular endothelial growth factor C (VEGF-C) gene microbubble transfection inhibits growth of MCF-7 breast cancer cells.
abstract: We evaluated the effects of ultrasound-mediated microbubble transfection of VEGF-C siRNA on breast cancer cells in vitro and in vivo. MCF-7 cells were transfected with VEGF-C siRNA and the protein and mRNA expression of VEGF-C was tested using Western blot and qRT-PCR. Twenty nude mice tumors were established by injecting with MCF-7 cells, and were randomized into four groups when palpable tumors reached 190 mm3. The length and width of MCF-7 tumors in mice were measured every 3 days. After 20 days, all mice were killed and the expression of VEGF-C in tumor tissue was also detected by Western blot and qRT-PCR. Results showed that VEGF-C siRNA effectively suppressed the protein and mRNA expression of VEGF-C in MCF-7 cells in vitro. VEGF-C siRNA inhibited the growth of human lymphatic endothelial cells (LECs) and MCF-7 cells. The volume and weight of MCF-7 tumor in VEGF-C siRNA microbubble with irradiation group were reduced with more extent than that in other groups in vivo. The present study highlights that VEGF-C siRNA in combination with ultrasound-mediated microbubble destruction (UMMD) could be a powerful, promising nonviral technology for breast cancer gene therapy.
authors.affiliation: Department of Ultrasound Imaging, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, PR China.
authors.forename: Qiuhua
authors.initials: Q
authors.lastname: Xu
authors.forename: Ting
authors.initials: T
authors.lastname: Sun
authors.forename: Hua
authors.initials: H
authors.lastname: Tian
authors.forename: Changqian
authors.initials: C
authors.lastname: Wang
authors.forename: Huihong
authors.initials: H
authors.lastname: Zhou
mesh_terms: D000818:Animals; D015153:Blotting, Western; D001943:Breast Neoplasms / Q000628:therapy*; D005260:Female; D015316:Genetic Therapy / Q000379:methods*; D006801:Humans; D061986:MCF-7 Cells; D051379:Mice; D008819:Mice, Nude; D045423:Microbubbles / Q000627:therapeutic use*; D034741:RNA, Small Interfering; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014162:Transfection / Q000379:methods*; D042582:Vascular Endothelial Growth Factor C / Q000096:biosynthesis / Q000235:genetics*; D023041:Xenograft Model Antitumor Assays
chemical_list: D034741:RNA, Small Interfering; C467486:VEGFC protein, human; D042582:Vascular Endothelial Growth Factor C