title: Anti-secretory and cyto-protective effects of peganine hydrochloride isolated from the seeds of Peganum harmala on gastric ulcers.
abstract: Gastroprotective mechanism of peganine hydrochloride isolated from Peganum harmala seeds was investigated. Peganine hydrochloride was evaluated against cold restraint (CRU), aspirin (AS), alcohol (AL) and pyloric ligation (PL) induced gastric ulcer models in rats. Potential anti-ulcer activity of peganine was observed against CRU (50.0%), AS (58.5%), AL (89.41%) and PL (62.50%) induced ulcer models. The reference drug omeprazole (10mg/kg, p.o.) showed 77.45% protection against CRU, 49.97% against AS and 69.42% against PL model. Sucralfate, another reference drug (500mg/kg, p.o.) showed 62.50% protection in AL induced ulcer model. Peganine significantly reduced free acidity (33.38%), total acidity (38.09%) and upregulated mucin secretion by 67.91%, respectively. Further, peagnine significantly inhibited H(+) K(+)-ATPase activity in vitro with IC50 of 73.47μg/ml as compared to the IC50 value of omeprazole (30.24μg/ml) confirming its anti-secretory activity.
authors.affiliation: Medicinal and Process Chemistry Division, CSIR-Central Drug Research Institute, Lucknow 226001, U.P., India.
authors.forename: Vinay Kumar
authors.initials: VK
authors.lastname: Singh
authors.forename: Vaibhav
authors.initials: V
authors.lastname: Mishra
authors.forename: Sriniwas
authors.initials: S
authors.lastname: Tiwari
authors.forename: Tanvir
authors.initials: T
authors.lastname: Khaliq
authors.forename: Manoj Kumar
authors.initials: MK
authors.lastname: Barthwal
authors.forename: Haushila Prasad
authors.initials: HP
authors.lastname: Pandey
authors.forename: Gautam
authors.initials: G
authors.lastname: Palit
authors.forename: Tadigoppula
authors.initials: T
authors.lastname: Narender
mesh_terms: D000438:Alcohols / Q000494:pharmacology; D000470:Alkaloids / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D000818:Animals; D000897:Anti-Ulcer Agents / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D000975:Antioxidants / Q000032:analysis / Q000378:metabolism; D001241:Aspirin / Q000494:pharmacology; D003080:Cold Temperature; D004195:Disease Models, Animal; D017506:H(+)-K(+)-Exchanging ATPase / Q000187:drug effects; D008297:Male; D015394:Molecular Structure; D009853:Omeprazole / Q000494:pharmacology; D032443:Peganum / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000627:therapeutic use*; D011708:Pylorus; D011799:Quinazolines / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012639:Seeds / Q000737:chemistry; D013276:Stomach Ulcer / Q000139:chemically induced / Q000188:drug therapy* / Q000517:prevention & control; D013392:Sucralfate / Q000494:pharmacology / Q000627:therapeutic use
chemical_list: D000438:Alcohols; D000470:Alkaloids; D000897:Anti-Ulcer Agents; D000975:Antioxidants; D010936:Plant Extracts; D011799:Quinazolines; D013392:Sucralfate; C019592:vasicine; D017506:H(+)-K(+)-Exchanging ATPase; D009853:Omeprazole; D001241:Aspirin
keywords: Gastric acid secretion; Gastric ulcer; Peganine hydrochloride; Peganum harmala; Proton pump