title: Expression and in Silico analysis of the recombinant bovine papillomavirus E6 protein as a model for viral oncoproteins studies.
abstract: Bovine papillomaviruses (BPVs) are recognized as the causal agents of economical relevant diseases in cattle, associated with the development of tumors in skin and mucosa. The oncogenesis process is mainly associated with different viral oncoprotein expressions, which are involved in cell transformation. The expression and characterization of recombinant viral oncoproteins represent an attractive strategy to obtain biotechnological products as antibodies and potential vaccines, Thus, the aim of this work was to clone and express the BPV-1 and BPV-2 E6 recombinant proteins and perform in silico analysis in order to develop a strategy for the systematic study of other papillomaviruses oncoproteins. The results demonstrated that BPV-1 and BPV-2 E6 recombinant proteins were expressed and purified from bacterial system as well as its in silico analysis was performed in order to explore and predict biological characteristics of these proteins.
authors.affiliation: Laboratório de Genética, Instituto Butantan, Secretaria de Estado da Saúde, Avenida Vital Brasil, 1500 Butantã, 05503-900 São Paulo, SP, Brazil.
authors.forename: J
authors.initials: J
authors.lastname: Mazzuchelli-de-Souza
authors.forename: R F
authors.initials: RF
authors.lastname: Carvalho
authors.forename: R M
authors.initials: RM
authors.lastname: Ruiz
authors.forename: T C
authors.initials: TC
authors.lastname: Melo
authors.forename: R P
authors.initials: RP
authors.lastname: Araldi
authors.forename: E
authors.initials: E
authors.lastname: Carvalho
authors.forename: C E
authors.initials: CE
authors.lastname: Thompson
authors.forename: M P
authors.initials: MP
authors.lastname: Sircili
authors.forename: W
authors.initials: W
authors.lastname: Beçak
authors.forename: R C
authors.initials: RC
authors.lastname: Stocco
mesh_terms: D000595:Amino Acid Sequence; D003001:Cloning, Molecular / Q000379:methods*; D008969:Molecular Sequence Data; D009856:Oncogene Proteins, Viral / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics*; D015202:Protein Engineering / Q000379:methods; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000737:chemistry*; D020539:Sequence Analysis, Protein / Q000379:methods*
chemical_list: D009856:Oncogene Proteins, Viral; D011994:Recombinant Proteins; C059269:protein E6, Bovine papillomavirus