title: A novel method to differentiate bovine and porcine gelatins in food products: nanoUPLC-ESI-Q-TOF-MS(E) based data independent acquisition technique to detect marker peptides in gelatin.
abstract: We presented a novel nanoUPLC-MS(E) workflow method that has potential to identify origin of gelatin in some dairy products; yoghurt, cheese and ice cream. In this study, the method was performed in two steps. In the first step, gelatin was extracted from these products before the MS-sample preparation. In the second step, tryptic gelatin peptides were separated and analyzed with ultra-performance liquid chromatography and electrospray ionization quadrupole time-of-flight mass spectrometry (nanoUPLC-ESI-q-TOF-MS(E)). The novelty of this setup was that it functioned in a data independent acquisition mode and that alternate low and elevated collision energy was applied to acquire precursor and product ion information. This enabled accurate mass acquisition on the peptide level to identify the gelatin peptides. The marker peptides specific for porcine and bovine could be successfully detected in the gelatin added to the dairy products analyzed, revealing that the detection of marker peptides in the digested gelatin samples using nanoUPLC-ESI-q-TOF-MS(E) could be an effective method to differentiate porcine and bovine gelatin in the dairy products.
authors.affiliation: Yıldız Technical University, Chemical and Metallurgical Engineering Faculty, Food Engineering Department, 34210 İstanbul, Turkey. mtyilmaz@yildiz.edu.tr
authors.forename: Mustafa Tahsin
authors.initials: MT
authors.lastname: Yilmaz
authors.forename: Zulal
authors.initials: Z
authors.lastname: Kesmen
authors.forename: Betul
authors.initials: B
authors.lastname: Baykal
authors.forename: Osman
authors.initials: O
authors.lastname: Sagdic
authors.forename: Oktay
authors.initials: O
authors.lastname: Kulen
authors.forename: Omer
authors.initials: O
authors.lastname: Kacar
authors.forename: Hasan
authors.initials: H
authors.lastname: Yetim
authors.forename: Ahmet Tarik
authors.initials: AT
authors.lastname: Baykal
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D015415:Biomarkers / Q000737:chemistry; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003611:Dairy Products / Q000032:analysis; D016002:Discriminant Analysis; D005780:Gelatin / Q000737:chemistry*; D008969:Molecular Sequence Data; D010449:Peptide Mapping; D010455:Peptides / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D013552:Swine; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D015415:Biomarkers; D010455:Peptides; D005780:Gelatin
keywords: Bovine; Dairy products; Gelatin; Porcine; Specification; nanoUPLC-ESI-q-TOF-MS(E) technique