title: A novel protein from Eupolyphaga sinensis inhibits adhesion, migration, and invasion of human lung cancer A549 cells.
abstract: Eupolyphaga sinensis Walker is an important insect used in Chinese traditional medicine. In this study, we purified a 72-kDa anticancer protein, designated as EPS72, from this species using ammonium sulfate precipitation, ultrafiltration, CM Sepharose Fast Flow cation exchange, Q Sepharose High Performance (HP) anion exchange, Butyl Sepharose HP hydrophobic chromatography, and Superdex 75 gel filtration chromatographic techniques. EPS72 exhibited a potent anticancer activity against the human lung cancer A549 cell line (IC50, 18.76 μg/mL). Further study showed that EPS72 could induce A549 cell detachment and apoptosis, inhibit cell adhesion to fibronectin and collagen IV, and restrain cell migration and invasion. Moreover, EPS72 significantly decreased the expression of β1-integrin. This study suggests that EPS72 could potentially be developed as a novel anticancer therapeutic agent due to its possible antimetastatic activity.
authors.affiliation: School of Pharmaceutical Sciences, Shandong University, Jinan, Shandong 250012, China.
authors.forename: Feng-xia
authors.initials: FX
authors.lastname: Wang
authors.forename: Ning
authors.initials: N
authors.lastname: Wu
authors.forename: Jian-teng
authors.initials: JT
authors.lastname: Wei
authors.forename: Jia
authors.initials: J
authors.lastname: Liu
authors.forename: Jin
authors.initials: J
authors.lastname: Zhao
authors.forename: Ai-guo
authors.initials: AG
authors.lastname: Ji
authors.forename: Xiu-kun
authors.initials: XK
authors.lastname: Lin
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis; D002448:Cell Adhesion / Q000187:drug effects*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects*; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D003058:Cockroaches; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D020128:Inhibitory Concentration 50; D019476:Insect Proteins / Q000032:analysis* / Q000737:chemistry; D007313:Insecta; D019012:Integrin beta1 / Q000378:metabolism; D008175:Lung Neoplasms / Q000188:drug therapy*; D009361:Neoplasm Invasiveness; D013997:Time Factors
chemical_list: D000970:Antineoplastic Agents; D019476:Insect Proteins; D019012:Integrin beta1