title: Distribution of AdeABC efflux system genes in genotypically diverse strains of clinical Acinetobacter baumannii.
abstract: Acinetobacter baumannii has emerged as a highly problematic hospital-associated pathogen. Different mechanisms contribute to the formation of multidrug resistance in A. baumannii, including the AdeABC efflux system. Distribution of the structural and regulatory genes encoding the AdeABC efflux system among genetically diverse clinical A. baumannii strains was achieved by using PCR and pulsed-field gel electrophoresis techniques. The distribution of adeABRS genes is extremely high among our A. baumannii strains, except the adeC gene. We have observed a large proportion of strains presenting multidrug-resistance phenotype for several years. The efflux pump could be an important mechanism in these strains in resistance to antibiotics.
authors.affiliation: Department of Microbiological Diagnostics and Infectious Immunology, Faculty of Pharmacy, Medical University of Bialystok, 15-225 Bialystok, Poland. Electronic address: piowie@umb.edu.pl.
authors.forename: Piotr
authors.initials: P
authors.lastname: Wieczorek
authors.forename: Paweł
authors.initials: P
authors.lastname: Sacha
authors.forename: Sławomir
authors.initials: S
authors.lastname: Czaban
authors.forename: Tomasz
authors.initials: T
authors.lastname: Hauschild
authors.forename: Dominika
authors.initials: D
authors.lastname: Ojdana
authors.forename: Oksana
authors.initials: O
authors.lastname: Kowalczuk
authors.forename: Robert
authors.initials: R
authors.lastname: Milewski
authors.forename: Bogusław
authors.initials: B
authors.lastname: Poniatowski
authors.forename: Jacek
authors.initials: J
authors.lastname: Nikliński
authors.forename: Elżbieta
authors.initials: E
authors.lastname: Tryniszewska
mesh_terms: D000151:Acinetobacter Infections / Q000382:microbiology*; D040981:Acinetobacter baumannii / Q000145:classification / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D024901:Drug Resistance, Multiple, Bacterial; D016521:Electrophoresis, Gel, Pulsed-Field; D005838:Genotype; D006801:Humans; D026901:Membrane Transport Proteins / Q000235:genetics* / Q000378:metabolism; D010802:Phylogeny
chemical_list: C492436:AdeA protein, Acinetobacter baumannii; C445684:AdeB protein, Acinetobacter baumannii; C492437:AdeC protein, Acinetobacter baumannii; D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D026901:Membrane Transport Proteins
keywords: Acinetobacter baumannii; AdeABC efflux pump genes; Pulsed-field gel electrophoresis