title: Integration sites and genotype distributions of human papillomavirus in cervical intraepithelial neoplasia.
abstract: OBJECTIVE
  To analyse HPV integration prevalence and genotype distributions in cervical intraepithelial neoplasia (CIN) in east part of China, furthermore to assess preferential sites for common HPV integrations and provide baseline information for cervical abnormality screening and prevention.
  
  
  METHODS
  Integration of HPV in 113 paraffin-embedded cervical intraepithelial neoplasia samples was assessed using Gencap technology in Key Laboratory of Biotechnologies in BGI-Shenzhen.
  
  
  RESULTS
  64 samples were HPV-integrated and as the cervical lesions increased, the integration rate became higher significantly (P=0.002). Fifteen different HPV genotypes were detected, 14 high-risk (16, 18, 31, 33, 51, 52, 56, 58, 66, 68) and 1 low-risk (11). The most common genotypes were HPV-16, 58, 33, 52, 66, and 56. Thirteen patients had co-integration involving mainly HPV-16 and 58. The frequency of HPV gene disruption was higher in L1 and E1 genes than in other regions of the viral genomes.
  
  
  CONCLUSIONS
  Some 56.6% of CIN lesions in Qingdao had HPV integrations, and 67.2% of HPV-integrated patients were HPV-16 and 58, more prone to be integrated in younger patients below 45 years old. There exist preferential sites for HPV-16 and HPV-58 integration, and they are more likely to be disrupted in the L1 and E1 loci.
authors.affiliation: Qingdao University Medical College, Qingdao, China. wlrst@126.com
authors.forename: Li
authors.initials: L
authors.lastname: Wang
authors.forename: Shu-Zhen
authors.initials: SZ
authors.lastname: Dai
authors.forename: Hui-Jun
authors.initials: HJ
authors.lastname: Chu
authors.forename: Hong-Fei
authors.initials: HF
authors.lastname: Cui
authors.forename: Xiao-Yan
authors.initials: XY
authors.lastname: Xu
mesh_terms: D000328:Adult; D000368:Aged; D004279:DNA, Viral / Q000235:genetics*; D005260:Female; D016679:Genome, Viral; D005838:Genotype; D006801:Humans; D008875:Middle Aged; D027383:Papillomaviridae / Q000145:classification / Q000235:genetics*; D030361:Papillomavirus Infections / Q000150:complications* / Q000235:genetics; D016612:Paraffin Embedding; D011379:Prognosis; D002583:Uterine Cervical Neoplasms / Q000209:etiology* / Q000473:pathology; D016662:Virus Integration / Q000235:genetics*; D002578:Uterine Cervical Dysplasia / Q000209:etiology* / Q000473:pathology
chemical_list: D004279:DNA, Viral