title: Protein and polysaccharide-composite sol-gel silicate film for an interference-free amperometric glucose biosensor.
abstract: A novel permselective, organic-inorganic-hybrid, sol-gel silicate-film was chemically modified on an anodized platinum (Pt) electrode surface to form a selective, sensitive and interference-free amperometric glucose biosensor. This permselective hybrid sol-gel film consists of three organo-silanes [i.e., 3-aminopropyltriethoxysilane (APTES); tetraethoxysilane (TEOS); triethoxy-1H,1H,2H,2H-tridecafluoro-n-octylsilane (FAS)] and two biomacromolecules [i.e., bovine serum albumin (BSA) and a chitosan (CHIT)]. After the addition of the film to the Pt electrode, glucose oxidase (GOx) was covalently immobilized within the film with glutaraldehyde. The incorporation of the BSA and CHIT not only enhanced the permselectivity of H2O2 but also improved the activity of the immobilized GOx. The CHIT effectively suppressed any swelling of the film. Moreover, the conjugation of the FAS was especially effective in reducing the interference currents of AA and UA to levels less than 1/400 and 1/300 of the current of H2O2. The resulting organic-inorganic-hybrid sol-gel-film-based amperometric glucose biosensor exhibited rapid and sensitive responses to glucose (100% response in <3s, sensitivity: 1.84 μA mM(-1), detection limit: 0.032 mM), and the highly selective determination of glucose was possible, even in the presence of 0.1mM AA and UA.
authors.affiliation: Department of Materials Science and Engineering, Graduate School of Engineering, Saitama Institute of Technology, Fukaya, Saitama 369-0293, Japan.
authors.forename: Hironori
authors.initials: H
authors.lastname: Matsuhisa
authors.affiliation: Department of Materials Science and Engineering, Graduate School of Engineering, Saitama Institute of Technology, Fukaya, Saitama 369-0293, Japan.
authors.forename: Munenori
authors.initials: M
authors.lastname: Tsuchiya
authors.affiliation: Department of Materials Science and Engineering, Graduate School of Engineering, Saitama Institute of Technology, Fukaya, Saitama 369-0293, Japan; Department of Life Science and Green Chemistry, Faculty of Engineering, Saitama Institute of Technology, Fukaya, Saitama 369-0293, Japan. Electronic address: hasebe@sit.ac.jp.
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Hasebe
mesh_terms: D000818:Animals; D001205:Ascorbic Acid / Q000032:analysis; D015374:Biosensing Techniques / Q000379:methods*; D002138:Calibration; D002417:Cattle; D048271:Chitosan / Q000737:chemistry; D004560:Electricity; D004563:Electrochemistry / Q000379:methods*; D004566:Electrodes; D005947:Glucose / Q000032:analysis*; D006801:Humans; D006861:Hydrogen Peroxide / Q000032:analysis; D008970:Molecular Weight; D010539:Permeability; D044367:Phase Transition; D011134:Polysaccharides / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D013053:Spectrophotometry; D013499:Surface Properties; D014527:Uric Acid / Q000032:analysis
chemical_list: D011134:Polysaccharides; D014527:Uric Acid; D012710:Serum Albumin, Bovine; D048271:Chitosan; D006861:Hydrogen Peroxide; D005947:Glucose; D001205:Ascorbic Acid
keywords: Amperometric glucose biosensor; Hydrogen peroxide; Interference-free; Organic–inorganic hybrid sol–gel film; Permselectivity