title: Identification of the soybean HyPRP family and specific gene response to Asian soybean rust disease.
abstract: Soybean [Glycine max (L.) Merril], one of the most important crop species in the world, is very susceptible to abiotic and biotic stress. Soybean plants have developed a variety of molecular mechanisms that help them survive stressful conditions. Hybrid proline-rich proteins (HyPRPs) constitute a family of cell-wall proteins with a variable N-terminal domain and conserved C-terminal domain that is phylogenetically related to non-specific lipid transfer proteins. Members of the HyPRP family are involved in basic cellular processes and their expression and activity are modulated by environmental factors. In this study, microarray analysis and real time RT-qPCR were used to identify putative HyPRP genes in the soybean genome and to assess their expression in different plant tissues. Some of the genes were also analyzed by time-course real time RT-qPCR in response to infection by Phakopsora pachyrhizi, the causal agent of Asian soybean rust disease. Our findings indicate that the time of induction of a defense pathway is crucial in triggering the soybean resistance response to P. pachyrhizi. This is the first study to identify the soybean HyPRP group B family and to analyze disease-responsive GmHyPRP during infection by P. pachyrhizi.
authors.affiliation: Programa de Pós-Graduação em Genética e Biologia Molecular, Departamento de Genética, Instituto de Biociências, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil .
authors.forename: Lauro Bücker
authors.initials: LB
authors.lastname: Neto
authors.forename: Rafael Rodrigues
authors.initials: RR
authors.lastname: de Oliveira
authors.forename: Beatriz
authors.initials: B
authors.lastname: Wiebke-Strohm
authors.forename: Marta
authors.initials: M
authors.lastname: Bencke
authors.forename: Ricardo Luís Mayer
authors.initials: RL
authors.lastname: Weber
authors.forename: Caroline
authors.initials: C
authors.lastname: Cabreira
authors.forename: Ricardo Vilela
authors.initials: RV
authors.lastname: Abdelnoor
authors.forename: Francismar Correa
authors.initials: FC
authors.lastname: Marcelino
authors.forename: Maria Helena Bodanese
authors.initials: MH
authors.lastname: Zanettini
authors.forename: Luciane Maria Pereira
authors.initials: LM
authors.lastname: Passaglia
keywords: Glycine max; HyPRP genes; fungal disease; real time RT-qPCR