title: Expression profiling based on graph-clustering approach to determine osteoarthritis related pathway.
abstract: BACKGROUND
  Osteoarthritis (OA) is the most common disease of joints in adults around the world. Current available drugs to treat osteoarthritis are predominantly directed towards the symptomatic relief of pain and inflammation but they do little to reduce joint destruction. Effective prevention of the structural damage must be a key objective of new therapeutic approaches. Therefore, it is worthwhile to search for important molecular markers that hold great promise for further treatment of patients with osteoarthritis.
  
  
  OBJECTIVE
  In this study, we used a graph-clustering approach to identify gene expression profiles that distinguish OA patients from normal samples.
  
  
  METHODS
  We performed a comprehensive gene level assessment of osteoarthritis using five osteoarthritis samples and five normal samples graph-clustering approach.
  
  
  RESULTS
  The results showed that TNFAIP3, ATF3, PPARG, etc, have related with osteoarthritis. Besides, we further mined the underlying molecular mechanism within these differently genes.
  
  
  CONCLUSIONS
  The results indicated tyrosine metabolism pathway and cell cycle pathway were two significant pathways, and there was evident to demonstrate them based on previous reports. We hope to provide insights into the development of novel therapeutic targets and pathways.
authors.affiliation: Department of Orthopedics, Chengdu Military General Hospital, Chengdu, Republic of China. zhangbodr@gmail.com  
authors.forename: B
authors.initials: B
authors.lastname: Zhang
authors.forename: Q-G
authors.initials: QG
authors.lastname: Xie
authors.forename: Y
authors.initials: Y
authors.lastname: Quan
authors.forename: X-M
authors.initials: XM
authors.lastname: Pan
mesh_terms: D051700:Activating Transcription Factor 3 / Q000235:genetics*; D002453:Cell Cycle; D016000:Cluster Analysis; D004268:DNA-Binding Proteins / Q000235:genetics*; D020869:Gene Expression Profiling; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics*; D009687:Nuclear Proteins / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis; D010003:Osteoarthritis / Q000235:genetics*; D047495:PPAR gamma / Q000235:genetics*; D000072598:Tumor Necrosis Factor alpha-Induced Protein 3; D014443:Tyrosine / Q000378:metabolism
chemical_list: C490449:ATF3 protein, human; D051700:Activating Transcription Factor 3; D004268:DNA-Binding Proteins; D047908:Intracellular Signaling Peptides and Proteins; D009687:Nuclear Proteins; D047495:PPAR gamma; D014443:Tyrosine; C076529:TNFAIP3 protein, human; D000072598:Tumor Necrosis Factor alpha-Induced Protein 3