title: [Effect of shenfu injection on CYP450s of rat liver].
abstract: The paper is to report the study of the effect of Shenfu injection on the enzyme activity of liver CYP450 and its mRNA level of rat liver. Microsome of rat liver was prepared after intravenous administration of Shenfu injection for 7 days. The enzyme activity was quantified by Cocktail method. Meanwhile, the mRNA expression of CYP1A2, CYP2B1/2, CYP2C11 and CYP3A1 in the liver was detected by RT-PCR. Shenfu injection obviously induced the enzyme activities of CYP2B and CYP2C. Meantime Shenfu injection decreased the enzyme activities of CYP1A2 and CYP3A. The mRNA levels of CYP2B and CYP2C were also induced in rats treated with Shenfu injection. But it obviously inhibited the mRNA level of CYP1A2 and CYP3A. Since the enzyme activity and mRNA level were obviously changed after administration, the potential effect of drug-drug interaction should be concerned.
authors.affiliation: Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China.
authors.forename: Han
authors.initials: H
authors.lastname: Li
authors.forename: Yu-Guang
authors.initials: YG
authors.lastname: Wang
authors.forename: Zeng-Chun
authors.initials: ZC
authors.lastname: Ma
authors.forename: Si-Si
authors.initials: SS
authors.lastname: Zhou
authors.forename: Qian-De
authors.initials: QD
authors.lastname: Liang
authors.forename: Cheng-Rong
authors.initials: CR
authors.lastname: Xiao
authors.forename: Hong-Ling
authors.initials: HL
authors.lastname: Tan
authors.forename: Xiang-Lin
authors.initials: XL
authors.lastname: Tang
authors.forename: Hua
authors.initials: H
authors.lastname: Li
authors.forename: Guo-Lin
authors.initials: GL
authors.lastname: Shen
authors.forename: Bo-Li
authors.initials: BL
authors.lastname: Zhang
authors.forename: Yue
authors.initials: Y
authors.lastname: Gao
mesh_terms: D031841:Aconitum / Q000737:chemistry*; D000818:Animals; D001189:Aryl Hydrocarbon Hydroxylases / Q000235:genetics / Q000378:metabolism; D019388:Cytochrome P-450 CYP1A2 / Q000235:genetics / Q000378:metabolism; D019362:Cytochrome P-450 CYP2B1 / Q000235:genetics / Q000378:metabolism; D051544:Cytochrome P-450 CYP3A / Q000235:genetics / Q000378:metabolism; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics / Q000378:metabolism*; D000072467:Cytochrome P450 Family 2; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D007267:Injections; D008297:Male; D008862:Microsomes, Liver / Q000201:enzymology*; D005894:Panax / Q000737:chemistry*; D010946:Plants, Medicinal / Q000737:chemistry; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D039181:Steroid 16-alpha-Hydroxylase / Q000235:genetics / Q000378:metabolism
chemical_list: D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D012333:RNA, Messenger; C492793:Shen-Fu; D003577:Cytochrome P-450 Enzyme System; D001189:Aryl Hydrocarbon Hydroxylases; C450279:CYP2C11 protein, rat; C465138:Cyp3a23-3a1 protein, rat; D019388:Cytochrome P-450 CYP1A2; D019362:Cytochrome P-450 CYP2B1; D051544:Cytochrome P-450 CYP3A; D000072467:Cytochrome P450 Family 2; D039181:Steroid 16-alpha-Hydroxylase