title: Neuroprotective effect of 2-(4-methoxyphenyl)ethyl-2-acetamido-2-deoxy-β-D-pyranoside against sodium nitroprusside-induced neurotoxicity in HT22 cells.
abstract: 2-(4-Methoxyphenyl) ethyl-2-acetamido-2-deoxy-β-D-pyranoside (GlcNAc-Sal), the salidroside analog was synthesized and shown to inhibit hypoglycemia and serum limitation induced apoptosis in PC12 cells. This study investigated the protective effects of GlcNAc-Sal on sodium nitroprusside (SNP)-induced cytotoxicity in HT22 cells. Cell viability tests and Hoechst 33342 staining comfirmed that GlcNAc-Sal pretreatment attenuated SNP-stimulated apoptotic cell death in HT22 cells in a concentration-dependent manner. The measurements of reactive oxygen species (ROS), nitric oxide (NO) production and apoptosis-related gene and protein expression suggest that the protection of GlcNAc-Sal, shown in this study, might be mediated by inhibiting intracellular ROS and NO production, and regulating apoptosis-related gene and protein expression during SNP stimulation. Perhaps, this study might contribute to the development of GlcNAc-Sal as an agent for preventing and/or treating a variety of NO-induced brain diseases.
authors.affiliation: Department of Pharmacology, Medical School, Nantong University, 19 Qixiu Road, Nantong, 226001, Jiangsu, China, jsxiaohuang@163.com.
authors.forename: Xia
authors.initials: X
authors.lastname: Chen
authors.forename: Aiqing
authors.initials: A
authors.lastname: Deng
authors.forename: Hongzhi
authors.initials: H
authors.lastname: Zhou
authors.forename: Jinhua
authors.initials: J
authors.lastname: Gu
mesh_terms: D000116:Acetylgalactosamine / Q000494:pharmacology*; D000117:Acetylglucosamine / Q000031:analogs & derivatives* / Q000494:pharmacology; D000818:Animals; D053148:Caspase 3 / Q000235:genetics / Q000378:metabolism; D016923:Cell Death / Q000187:drug effects; D002460:Cell Line; D019610:Cytoprotection / Q000187:drug effects; D005786:Gene Expression Regulation / Q000187:drug effects; D051379:Mice; D018696:Neuroprotective Agents / Q000494:pharmacology*; D009498:Neurotoxins / Q000633:toxicity*; D009569:Nitric Oxide / Q000096:biosynthesis; D009599:Nitroprusside / Q000633:toxicity*; D011065:Poly(ADP-ribose) Polymerases / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D051028:bcl-2-Associated X Protein / Q000235:genetics / Q000378:metabolism
chemical_list: C000589286:2-(4-methoxyphenyl)ethyl-2-acetamido-2-deoxyglucopyranoside; D018696:Neuroprotective Agents; D009498:Neurotoxins; D012333:RNA, Messenger; D017382:Reactive Oxygen Species; D051028:bcl-2-Associated X Protein; D009599:Nitroprusside; D009569:Nitric Oxide; D011065:Poly(ADP-ribose) Polymerases; D053148:Caspase 3; D000116:Acetylgalactosamine; D000117:Acetylglucosamine