title: Dynamic QuantiFERON Response in Psoriasis Patients Taking Long-Term Biologic Therapy.
abstract: BACKGROUND
  The risk of active tuberculosis is increased in psoriasis patients receiving biologic drug therapy. The QuantiFERON-TB Gold In-Tube assay (QFT) is used for latent tuberculosis screening in these patients. This study presents a retrospective analysis on repeated QFT assays, investigating the influence of biologic drugs and isoniazid therapy on the outcome of the assay.
  
  
  METHODS
  Serial QFTs of 58 psoriasis patients, who received biologic drug therapy, were evaluated at baseline and after 12 months of treatment. Patients were retrospectively divided in four groups according to QFT results at baseline and at follow-up: patients having a QFT reversion (from positive to negative results); patients with a conversion (from negative to positive); patients confirming the baseline results, either positive or negative.
  
  
  RESULTS
  At the end of the 12-months period, 11.1% of patients with a negative QFT result at baseline presented a conversion, showing low interferon (IFN)-gamma values, whereas 6.9% of positive patients presented a QFT reversion. When the test was repeated after 2-3 months without isoniazid chemoprophylaxis, patients with QFT conversion showed negative results. No patient developed active tuberculosis.
  
  
  CONCLUSIONS
  In patients undergoing biologic therapy, a positive QFT assay needs to be further confirmed, as false-positive results may occur after long-term therapy. Repeating QFT tests in patients with low IFN-gamma values could reduce the incidence of false-positive latent tuberculosis infection diagnosis, thus preventing unnecessary tuberculosis chemoprophylaxis. In conclusion, a dynamic QFT response is possible in psoriasis patients undergoing biologic therapy.
authors.affiliation: Laboratory of Clinical-Chemistry and Microbiology, IRCCS Galeazzi Orthopedic Institute, Milan, Italy ; Department of Biomedical Sciences for Health, University of Milan, Milan, Italy.
authors.forename: Lorenzo
authors.initials: L
authors.lastname: Drago
authors.forename: Lucia
authors.initials: L
authors.lastname: Nicola
authors.forename: Valentina
authors.initials: V
authors.lastname: Signori
authors.forename: Elisa
authors.initials: E
authors.lastname: Palazzi
authors.forename: Cecilia
authors.initials: C
authors.lastname: Garutti
authors.forename: Samantha
authors.initials: S
authors.lastname: Spadino
authors.forename: Gianfranco
authors.initials: G
authors.lastname: Altomare
keywords: Biologic drugs; Chemoprophylaxis; Dermatology; False positive; Isoniazid; Psoriasis; QuantiFERON; Tuberculosis