title: Biochemical investigation of kraft lignin degradation by Pandoraea sp. B-6 isolated from bamboo slips.
abstract: Kraft lignin (KL) is the major pollutant in black liquor. The bacterial strain Pandoraea sp. B-6 was able to degrade KL without any co-substrate under high alkaline conditions. At least 38.2 % of chemical oxygen demand and 41.6 % of color were removed in 7 days at concentrations from 1 to 6 g L(-1). The optimum pH for KL degradation was 10 and the optimum temperature was 30 °C. The greatest activities of 2,249.2 U L(-1) for manganese peroxidase and 1,120.6 U L(-1) for laccase were detected on the third and fifth day at pH 10, respectively. Many small molecules, such as cinnamic acid, ferulic acid, 2-hydroxy benzyl alcohol, and vanillyl methyl ketone, were formed during the period of KL degradation based on GC-MS analysis. These results indicate that this strain has great potential for biotreatment of black liquor.
authors.affiliation: School of Metallurgical Science and Engineering, Central South University, Changsha, 410017, Hunan, People's Republic of China, shiyzy@126.com.
authors.forename: Yan
authors.initials: Y
authors.lastname: Shi
authors.forename: Liyuan
authors.initials: L
authors.lastname: Chai
authors.forename: Chongjian
authors.initials: C
authors.lastname: Tang
authors.forename: Zhihui
authors.initials: Z
authors.lastname: Yang
authors.forename: Yu
authors.initials: Y
authors.lastname: Zheng
authors.forename: Yuehui
authors.initials: Y
authors.lastname: Chen
authors.forename: Qingxiu
authors.initials: Q
authors.lastname: Jing
mesh_terms: D031723:Bambusa / Q000378:metabolism*; D001681:Biological Assay; D057919:Biological Oxygen Demand Analysis; D019117:Burkholderia / Q000254:growth & development / Q000378:metabolism*; D003116:Color; D008401:Gas Chromatography-Mass Spectrometry; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D008031:Lignin / Q000378:metabolism*; D013696:Temperature
chemical_list: D008031:Lignin