title: [Recombinant strain producing thermostable lipase from Thermomyces lanuginosus immobilized into nanocarbon silica matrices and properties of the prepared biocatalyzers].
abstract: Multicomponent composite biocatalyzers with lipolytic activity have been studied. These biocatalyzers were prepared through the immobilization of a recombinant producer strain of thermostable lipase from Thermomyces lanuginosus into SiO2 xerogel, which contains a nanocarbon component, i.e., multilayered carbon nanotubes with varying diameters, and also bulblike structured carbon nanospheres ("nanobulb"). The properties of lipase were studied both in cell suspensions of a recombinant producer strain constructed based on E. coli BL21(DE3) and in the immobilized state with regard to the structure and dispersibility of the nanocarbon component used in the composition of the biocatalyzers. It was shown that the recombinant intracellular lipase exerted its activity in a reaction of tributirin hydrolysis on average comprising 50 U/mg of dried cells and had a high level of thermostability. Upon heating in olive oil at 100 degrees C, the inactivation constant and the period of semi-inactivation comprised 6 x 10(-3) min(-1) and 2 h, respectively, exceeding by one order the thermostability of lipase in a buffer solution. Biocatalyzers that contained aggregated "thick" nanotubes with a diameter of 20-22 nm had the maximum initial activity-250 U/g.
authors.forename: G A
authors.initials: GA
authors.lastname: Kovalenko
authors.forename: A B
authors.initials: AB
authors.lastname: Beklemishev
authors.forename: L V
authors.initials: LV
authors.lastname: Perminova
authors.forename: T V
authors.initials: TV
authors.lastname: Chuenko
authors.forename: I D
authors.initials: ID
authors.lastname: Ivanov
authors.forename: S I
authors.initials: SI
authors.lastname: Moiseenkov
authors.forename: V L
authors.initials: VL
authors.lastname: Kuznetsov
mesh_terms: D001203:Ascomycota / Q000201:enzymology*; D001426:Bacterial Proteins; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D006868:Hydrolysis; D007700:Kinetics; D008049:Lipase / Q000737:chemistry / Q000235:genetics*; D037742:Nanotubes, Carbon / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry
chemical_list: D001426:Bacterial Proteins; D004800:Enzymes, Immobilized; D037742:Nanotubes, Carbon; D012822:Silicon Dioxide; D008049:Lipase; C428386:thermostable lipase