title: Capillary gel electrophoresis for the quantification and purity determination of recombinant proteins in inclusion bodies.
abstract: In this work, a high-resolution CGE method for quantification and purity determination of recombinant proteins was developed, involving a single-component inclusion bodies (IBs) solubilization solution. Different recombinant proteins expressed as IBs were used to show method capabilities, using recombinant interferon-β 1b as the model protein for method validation. Method linearity was verified in the range from 0.05 to 0.40 mg/mL and a determination coefficient (r(2) ) of 0.99 was obtained. The LOQs and LODs were 0.018 and 0.006 mg/mL, respectively. RSD for protein content repeatability test was 2.29%. In addition, RSD for protein purity repeatability test was 4.24%. Method accuracy was higher than 90%. Specificity was confirmed, as the method was able to separate recombinant interferon-β 1b monomer from other aggregates and impurities. Sample content and purity was demonstrated to be stable for up to 48 h. Overall, this method is suitable for the analysis of recombinant proteins in IBs according to the attributes established on the International Conference for Harmonization guidelines.
authors.affiliation: Unidad de Investigación y Desarrollo, Probiomed S.A. de C.V., Tenancingo, Estado de México, México.
authors.forename: Carlos E
authors.initials: CE
authors.lastname: Espinosa-de la Garza
authors.forename: Francisco C
authors.initials: FC
authors.lastname: Perdomo-Abúndez
authors.forename: Víctor R
authors.initials: VR
authors.lastname: Campos-García
authors.forename: Néstor O
authors.initials: NO
authors.lastname: Pérez
authors.forename: Luis F
authors.initials: LF
authors.lastname: Flores-Ortiz
authors.forename: Emilio
authors.initials: E
authors.lastname: Medina-Rivero
mesh_terms: D019075:Electrophoresis, Capillary / Q000379:methods*; D006801:Humans; D002479:Inclusion Bodies / Q000737:chemistry*; D000068576:Interferon beta-1b; D016899:Interferon-beta / Q000737:chemistry; D016014:Linear Models; D008956:Models, Chemical; D011994:Recombinant Proteins / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012995:Solubility
chemical_list: D011994:Recombinant Proteins; D000068576:Interferon beta-1b; D016899:Interferon-beta
keywords: CGE; Inclusion bodies; Interferon-β 1b; Validation