title: Assessing the fecal microbiota: an optimized ion torrent 16S rRNA gene-based analysis protocol.
abstract: Assessing the distribution of 16S rRNA gene sequences within a biological sample represents the current state-of-the-art for determination of human gut microbiota composition. Advances in dissecting the microbial biodiversity of this ecosystem have very much been dependent on the development of novel high-throughput DNA sequencing technologies, like the Ion Torrent. However, the precise representation of this bacterial community may be affected by the protocols used for DNA extraction as well as by the PCR primers employed in the amplification reaction. Here, we describe an optimized protocol for 16S rRNA gene-based profiling of the fecal microbiota.
authors.affiliation: Laboratory of Probiogenomics, Department of Life Sciences, University of Parma, Italy.
authors.forename: Christian
authors.initials: C
authors.lastname: Milani
authors.forename: Arancha
authors.initials: A
authors.lastname: Hevia
authors.forename: Elena
authors.initials: E
authors.lastname: Foroni
authors.forename: Sabrina
authors.initials: S
authors.lastname: Duranti
authors.forename: Francesca
authors.initials: F
authors.lastname: Turroni
authors.forename: Gabriele Andrea
authors.initials: GA
authors.lastname: Lugli
authors.forename: Borja
authors.initials: B
authors.lastname: Sanchez
authors.forename: Rebeca
authors.initials: R
authors.lastname: Martín
authors.forename: Miguel
authors.initials: M
authors.lastname: Gueimonde
authors.forename: Douwe
authors.initials: D
authors.lastname: van Sinderen
authors.forename: Abelardo
authors.initials: A
authors.lastname: Margolles
authors.forename: Marco
authors.initials: M
authors.lastname: Ventura
mesh_terms: D000818:Animals; D044822:Biodiversity; D005243:Feces / Q000382:microbiology*; D005260:Female; D006801:Humans; D064307:Microbiota; D012336:RNA, Ribosomal, 16S / Q000737:chemistry / Q000235:genetics*; D051381:Rats; D017422:Sequence Analysis, DNA / Q000379:methods*
chemical_list: D012336:RNA, Ribosomal, 16S