title: A yellow fluorescent protein with reduced chloride sensitivity engineered by loop-insertion.
abstract: Light it up! We demonstrate a novel way to reduce the chloride sensitivity of yellow fluorescent protein by inserting glycine residues in its loop region. The length and position for the insertion were optimized experimentally, and a plausible underlying mechanism is proposed.
authors.affiliation: National Laboratory of Solid State Microstructure, Department of Physics, Nanjing University, Jiangsu 210008, China.
authors.forename: Junyi
authors.initials: J
authors.lastname: Liang
authors.forename: Yang
authors.initials: Y
authors.lastname: Yang
authors.forename: Puguang
authors.initials: P
authors.lastname: Yin
authors.forename: Yin
authors.initials: Y
authors.lastname: Ding
authors.forename: Yan
authors.initials: Y
authors.lastname: Shen
authors.forename: Meng
authors.initials: M
authors.lastname: Qin
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
authors.forename: Qiang
authors.initials: Q
authors.lastname: Xu
authors.forename: Yi
authors.initials: Y
authors.lastname: Cao
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics; D002712:Chlorides / Q000737:chemistry*; D005998:Glycine / Q000737:chemistry / Q000235:genetics; D008164:Luminescent Proteins / Q000737:chemistry* / Q000235:genetics; D016254:Mutagenesis, Insertional; D015202:Protein Engineering
chemical_list: D001426:Bacterial Proteins; D002712:Chlorides; D008164:Luminescent Proteins; C054562:yellow fluorescent protein, Bacteria; D005998:Glycine
keywords: fluorescent protein; imaging; photo stability; protein design; protein engineering