title: Protection of vascular endothelial cells injured by angiotensin II and hypoxia in vitro by Ginkgo biloba (Ginaton).
abstract: The objective of this study was to explore the protective effect and the possible mechanism of Ginkgo biloba extract (Ginaton) on human vascular endothelial cells (VECs) injured by angiotensin II (Ang-II) and hypoxia. The human aortic VECs were divided into different groups to observe the changes in endothelin (ET), calcium concentration ([Ca(2+)]i), and mitochondrial membrane potential (MMP). The results showed that Ginaton had inhibited ET secretion induced by hypoxia and Ang-II (P < .01). the protection offered by Ginaton at mid (10 mg/mL) and low (5 mg/mL) concentrations was obviously better than that offered at high concentration (25 mg/mL). The [Ca(2+)]i increased and MMP decreased significantly in both hypoxia group and Ang-II group (P < .01); however, the changes in [Ca(2+)]i and MMP could be meliorated by Ginaton. This study suggested that Ginaton could effectively protect VECs against injury, and the dose used clinically would rather be low than too high for getting better results.
authors.affiliation: 1Aerospace and Diving Medical Center of Navy General Hospital, Beijing, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Han
authors.forename: Minggao
authors.initials: M
authors.lastname: Li
mesh_terms: D000804:Angiotensin II / Q000633:toxicity*; D002118:Calcium / Q000378:metabolism; D015687:Cell Hypoxia; D002478:Cells, Cultured; D019610:Cytoprotection; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D042783:Endothelial Cells / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D016232:Endothelins / Q000378:metabolism; D020441:Ginkgo biloba; D006801:Humans; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D008517:Phytotherapy; D010946:Plants, Medicinal; D020011:Protective Agents / Q000494:pharmacology*; D013997:Time Factors
chemical_list: D004365:Drugs, Chinese Herbal; D016232:Endothelins; D020011:Protective Agents; C526713:ginaton; D000804:Angiotensin II; D002118:Calcium
keywords: Ang-II; Ginaton; calcium concentration; hypoxia; mitochondrial membrane potential; vascular endothelial cells