title: (32)P-postlabelling/HPLC analysis of various styrene-induced DNA adducts in mice.
abstract: Styrene oxide (SO), a reactive metabolite of styrene, modifies DNA at several nucleophilic sites. In the present work we have determined the SO-DNA adducts in vitro and in vivo by two different versions of (32)P-postlabelling/HPLC assays. When anionexchange cartridges were used for adduct enrichment the β-isomer of 7-substituted guanines was detected in in vitro SO-treated DNA as well as in mice lungs exposed to styrene at 750 and 1500 mg m(-3) for 21 days (6 h day(-1), 7 days week(-1)). In the lungs, the adduct levels were 6.5 and 23 per 10(8) nucleotides for the two doses, respectively. When the nuclease P1 resistant adducts were studied by the (32)P-postlabelling/HPLC assay involving nuclease P1/prostatic acid phosphatase hydrolysis, the main adducts in in vitro-treated DNA were the α-isomer of N(2)-substituted guanine, β-isomers of 1-substituted adenine and 3-substituted uracil. β1-SO-adenine adduct was detected in the mice lung tissues after conversion of the 1-substituted adduct to the βN(6)-SO-adenine adduct by the Dimroth rearrangement. The 1-adenine adduct levels for the two doses were found to be 0.17 and 0.51 per 10(8) nucleotides. The current results show the potential of using the 7-guanine and 1-adenine adducts as biomarkers in biomonitoring of styrene exposure.
authors.affiliation: Center for Nutrition and Toxicology, Department of Biosciences at Novum, Karolinska Institute, SE-141 57 Huddinge, Sweden. e-mail: mikko.koskinen@cnt.ki.se.
authors.forename: M
authors.initials: M
authors.lastname: Koskinen
authors.forename: P
authors.initials: P
authors.lastname: Vodička
authors.forename: L
authors.initials: L
authors.lastname: Vodičkova
authors.forename: K
authors.initials: K
authors.lastname: Hemminki