title: Does centrifugation and semen processing with swim up at 37°C yield sperm with better DNA integrity compared to centrifugation and processing at room temperature?
abstract: OBJECTIVE
  To evaluate whether semen processing at 37°C yield sperm with better DNA integrity compared to centrifugation and processing at room temperature (RT) by swim-up method.
  
  
  METHODS
  This study was done at tertiary care center attached to Reproductive Medicine Unit and Medical College.
  
  
  METHODS
  Prospective pilot study.
  
  
  METHODS
  Normozoospermic men (n = 50) undergoing diagnostic semen analysis.
  
  
  METHODS
  Normozoospermic samples (World Health Organization, 2010 criteria) after analysis was divided into two aliquots (0.5 mL each); one was processed at 37°C and the other at RT by swim-up method. DNA fragmentation of both samples post wash was calculated by acridine orange method.
  
  
  METHODS
  The values of sperm DNA fragmentation were represented as mean and standard error (mean ± SEM) of the mean. Paired t-test was used for calculating the sperm DNA integrity difference between post wash at RT and 37°C.
  
  
  RESULTS
  Statistically significant difference was not observed in post wash sperm DNA fragmentation values at 37°C compared to RT.
  
  
  CONCLUSIONS
  Our data represents that there was no significant difference in sperm DNA fragmentation values of samples processed at 37°C and at RT. Hence, sperm processing at 37°C does not yield sperm with better DNA integrity compared to centrifugation and processing at RT.
authors.affiliation: Department of Reproductive Medicine, Mohak Hi-tech Speciality Laparoscopy and Infertility Centre, Sri Aurobindo Institute of Medical Sciences, Indore, Madhya Pradesh, India.
authors.forename: Deepthi
authors.initials: D
authors.lastname: Repalle
authors.forename: Priya Bhave
authors.initials: PB
authors.lastname: Chittawar
authors.forename: Shilpa
authors.initials: S
authors.lastname: Bhandari
authors.forename: Geetanjali
authors.initials: G
authors.lastname: Joshi
authors.forename: Mansi
authors.initials: M
authors.lastname: Paranjape
authors.forename: Charudutta
authors.initials: C
authors.lastname: Joshi
keywords: Acridine orange; semen preparation; sperm DNA integrity; temperature