title: MYB transcription factors regulate glucosinolate biosynthesis in different organs of Chinese cabbage (Brassica rapa ssp. pekinensis).
abstract: In this study, we investigated the expression of seven MYB transcription factors (a total of 17 genes that included Dof1.1, IQD1-1, MYB28, MYB29, MYB34, MYB51, and MYB122 and their isoforms) involved in aliphatic and indolic glucosinolate (GSL) biosynthesis and analyzed the aliphatic and indolic GSL content in different organs of Chinese cabbage (Brassica rapassp. Pekinensis). MYB28 and MYB29 expression in the stem was dramatically different when compared with the levels in the other organs. MYB34, MYB122, MYB51, Dof1.1, and IQD1-1 showed very low transcript levels among different organs. HPLC analysis showed that the glucosinolates (GSLs) consisted of five aliphatic GSLs (progoitrin, sinigrin, glucoalyssin, gluconapin, and glucobrassicanapin) and four indolic GSLs (4-hydroxyglucobrassicin, glucobrassicin, 4-methoxygluco-brassicin, and neoglucobrassicin). Aliphatic GSLs exhibited 63.3% of the total GSLs content, followed by aromatic GSL (19.0%), indolic GSLs (10%), and unknown GSLs (7.7%) in different organs of Chinese cabbage. The total GSL content of different parts (ranked in descending order) was as follows: seed > flower > young leaves > stem > root > old leaves. The relationship between GSLs accumulation and expression of GSLs biosynthesis MYB TFs genes in different organs may be helpful to understand the mechanism of MYB TFs regulating GSL biosynthesis in Chinese cabbage.
authors.affiliation: Department of Crop Science, Chungnam National University, 99 Daehak-ro, Yuseong-gu, Daejeon 305-764, Korea. yeonbokkim@hanmail.net
authors.forename: Yeon Bok
authors.initials: YB
authors.lastname: Kim
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Li
authors.forename: Sun-Ju
authors.initials: SJ
authors.lastname: Kim
authors.forename: Haeng Hoon
authors.initials: HH
authors.lastname: Kim
authors.forename: Jeongyeo
authors.initials: J
authors.lastname: Lee
authors.forename: HyeRan
authors.initials: H
authors.lastname: Kim
authors.forename: Sang Un
authors.initials: SU
authors.lastname: Park
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000378:metabolism; D029741:Brassica rapa / Q000235:genetics / Q000378:metabolism*; D002148:Calmodulin-Binding Proteins / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism; D035264:Flowers / Q000235:genetics / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D005961:Glucosinolates / Q000096:biosynthesis*; D051548:Histone Acetyltransferases / Q000378:metabolism; D010802:Phylogeny; D018515:Plant Leaves / Q000235:genetics / Q000378:metabolism; D018517:Plant Roots / Q000235:genetics / Q000378:metabolism; D018547:Plant Stems / Q000235:genetics / Q000378:metabolism; D012639:Seeds / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000145:classification / Q000378:metabolism*; D000099224:p300-CBP-Associated Factor
chemical_list: D029681:Arabidopsis Proteins; D002148:Calmodulin-Binding Proteins; D004268:DNA-Binding Proteins; D005961:Glucosinolates; D051548:Histone Acetyltransferases; D014157:Transcription Factors; C407526:DAG1 protein, Arabidopsis; C503424:IQD1 protein, Arabidopsis; C523452:MYB51 protein, Arabidopsis; C523552:Myb29 protein, Arabidopsis; C474636:GCN5 protein, Arabidopsis; D000099224:p300-CBP-Associated Factor