title: Quantitative determination and pattern recognition analyses of bioactive marker compounds from Dipsaci Radix by HPLC.
abstract: In this study, quantitative and pattern recognition analyses were developed using HPLC/UV for the quality evaluation of Dipsaci Radix. For quantitative analysis, five major bioactive compounds were assessed. The separation conditions employed for HPLC/UV were optimized using ODS C18 column (250 × 4.6 mm, 5 μm) with a gradient of acetonitrile and water as the mobile phase at a flow rate of 1.0 mL/min and a detection wavelength of 212 nm. These methods were fully validated with respect to linearity, accuracy, precision, recovery, and robustness. The HPLC/UV method was applied successfully to the quantification of five major compounds in the extract of Dipsaci Radix. The HPLC analytical method for pattern recognition analysis was validated by repeated analysis of 17 Dipsaci Radix and four Phlomidis Radix samples. The results indicate that the established HPLC/UV method is suitable for quantitative analysis.
authors.affiliation: College of Pharmacy, Catholic University of Daegu, Gyeongsan, 712-702, Korea.
authors.forename: Bing Tian
authors.initials: BT
authors.lastname: Zhao
authors.forename: Su Yang
authors.initials: SY
authors.lastname: Jeong
authors.forename: Dong Cheul
authors.initials: DC
authors.lastname: Moon
authors.forename: Kun Ho
authors.initials: KH
authors.lastname: Son
authors.forename: Jong Keun
authors.initials: JK
authors.lastname: Son
authors.forename: Mi Hee
authors.initials: MH
authors.lastname: Woo
mesh_terms: D049917:Dipsacaceae / Q000737:chemistry*; D004365:Drugs, Chinese Herbal / Q000032:analysis* / Q000737:chemistry*; D015394:Molecular Structure; D010363:Pattern Recognition, Automated; D018517:Plant Roots / Q000737:chemistry
chemical_list: D004365:Drugs, Chinese Herbal