title: A novel pattern recognition protein of the Chinese oak silkmoth, Antheraea pernyi, is involved in the pro-PO activating system.
abstract: In this paper, we firstly reported a C-type lectin cDNA clone of 1029 bps from the larvae of A. Pernyi (Ap-CTL) using PCR and RACE techniques. The full-length cDNA contains an open reading frame encoding 308 amino acid residues which has two different carbohydrate-recognition domains (CRDs) arranged in tandem. To investigate the biological activities in the innate immunity, recombinant Ap-CTL was expressed in E. coli with a 6-histidine at the amino-terminus (Ap-rCTL). Besides acted as a broad-spectrum recognition protein binding to a wide range of PAMPs and microorganisms, Ap-rCTL also had the ability to recognize and trigger the agglutination of bacteria and fungi. In the proPO activation assay, Ap-rCTL specifically restored the PO activity of hemolymph blocked by anti- Ap-rCTL antibody in the presence of different PAMPs or microorganisms. In summary, Ap-rCTL plays an important role in insect innate immunity as an pattern recognition protein.
authors.affiliation: School of Life Science and Bio-pharmaceutics, Shenyang Pharmaceutical University, Shenyang, Liaoning Province 110016, PR China.
authors.forename: Wang
authors.initials: W
authors.lastname: Xialu
authors.forename: Zhang
authors.initials: Z
authors.lastname: Jinghai
authors.forename: Chen
authors.initials: C
authors.lastname: Ying
authors.forename: Ma
authors.initials: M
authors.lastname: Youlei
authors.forename: Zou
authors.initials: Z
authors.lastname: Wenjun
authors.forename: Ding
authors.initials: D
authors.lastname: Guoyuan
authors.forename: Li
authors.initials: L
authors.lastname: Wei
authors.forename: Zhao
authors.initials: Z
authors.lastname: Mingyi
authors.forename: Wu
authors.initials: W
authors.lastname: Chunfu
authors.forename: Zhang
authors.initials: Z
authors.lastname: Rong
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002681:China; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000378:metabolism; D007113:Immunity, Innate; D037181:Lectins, C-Type / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D009036:Moths / Q000276:immunology / Q000378:metabolism; D017856:Phosphotransferases (Phosphate Group Acceptor) / Q000378:metabolism; D011485:Protein Binding; D012333:RNA, Messenger / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics; D016415:Sequence Alignment
chemical_list: D037181:Lectins, C-Type; D012333:RNA, Messenger; D011993:Recombinant Fusion Proteins; D017856:Phosphotransferases (Phosphate Group Acceptor); C059169:polyphosphate AMP phosphotransferase