title: Field-based flow cytometry for ex vivo characterization of Plasmodium vivax and P. falciparum antimalarial sensitivity.
abstract: Ex vivo antimalarial sensitivity testing in human malaria parasites has largely depended on microscopic determination of schizont maturation. While this microscopic method is sensitive, it suffers from poor precision and is laborious. The recent development of portable, low-cost cytometers has allowed us to develop and validate a simple, field-optimized protocol using SYBR green and dihydroethidium for the accurate and objective determination of antimalarial drug sensitivity in freshly isolated Plasmodium vivax and Plasmodium falciparum.
authors.affiliation: Department of Microbiology, Yong Loo Lin School of Medicine, National University of Singapore, National University Health System, Singapore.
authors.forename: B
authors.initials: B
authors.lastname: Russell
authors.forename: B
authors.initials: B
authors.lastname: Malleret
authors.forename: R
authors.initials: R
authors.lastname: Suwanarusk
authors.forename: C
authors.initials: C
authors.lastname: Anthony
authors.forename: S
authors.initials: S
authors.lastname: Kanlaya
authors.forename: Y L
authors.initials: YL
authors.lastname: Lau
authors.forename: C J
authors.initials: CJ
authors.lastname: Woodrow
authors.forename: F
authors.initials: F
authors.lastname: Nosten
authors.forename: L
authors.initials: L
authors.lastname: Renia
mesh_terms: D000962:Antimalarials / Q000494:pharmacology*; D005434:Flow Cytometry / Q000379:methods*; D010963:Plasmodium falciparum / Q000187:drug effects*; D010966:Plasmodium vivax / Q000187:drug effects*
chemical_list: D000962:Antimalarials