title: Global transcriptomic analysis of human neuroblastoma cells in response to enterovirus type 71 infection.
abstract: Human enterovirus type 71 (EV71) is the major pathogen of hand-foot-and-mouth disease (HFMD) and has been associated with severe neurological disease and even death in infants and young children. The pathogenesis of EV71 infection in the human central nervous system remains unclear. In this study, human whole genome microarray was employed to perform transcriptome profiling in SH-SY5Y human neuroblastoma cells infected with EV71. The results indicated that EV71 infection lead to altered expression of 161 human mRNAs, including 74 up-regulated genes and 87 down-regulated genes. Bioinformatics analysis indicated the possible roles of the differentially regulated mRNAs in selected pathways, including cell cycle/proliferation, apoptosis, and cytokine/chemokine responses. Finally, the microarray results were validated using real-time RT-PCR with high identity. Overall, our results provided fundamental information regarding the host response to EV71 infection in human neuroblastoma cells, and this finding will help explain the pathogenesis of EV71 infection and virus-host interaction.
authors.affiliation: Department of Virology, State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, China.
authors.forename: Li-Juan
authors.initials: LJ
authors.lastname: Xu
authors.forename: Tao
authors.initials: T
authors.lastname: Jiang
authors.forename: Fu-Jun
authors.initials: FJ
authors.lastname: Zhang
authors.forename: Jian-Feng
authors.initials: JF
authors.lastname: Han
authors.forename: Juan
authors.initials: J
authors.lastname: Liu
authors.forename: Hui
authors.initials: H
authors.lastname: Zhao
authors.forename: Xiao-Feng
authors.initials: XF
authors.lastname: Li
authors.forename: Rui-Ju
authors.initials: RJ
authors.lastname: Liu
authors.forename: Yong-Qiang
authors.initials: YQ
authors.lastname: Deng
authors.forename: Xiao-Yan
authors.initials: XY
authors.lastname: Wu
authors.forename: Shun-Ya
authors.initials: SY
authors.lastname: Zhu
authors.forename: E-De
authors.initials: ED
authors.lastname: Qin
authors.forename: Cheng-Feng
authors.initials: CF
authors.lastname: Qin
mesh_terms: D045744:Cell Line, Tumor; D016000:Cluster Analysis; D029821:Enterovirus A, Human / Q000502:physiology*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006801:Humans; D058977:Molecular Sequence Annotation; D009447:Neuroblastoma / Q000235:genetics* / Q000821:virology*; D015203:Reproducibility of Results; D056189:Viral Tropism