title: Diagnosis of essential hypertension in humans by the determination of plasma renal cortexin using enzyme-linked immunosorbent assay.
abstract: BACKGROUND
  More than 90% of all hypertensive persons are reported to have essential hypertension (EH), a particular form of elevated blood pressure, for which no diagnostic test is currently available. The level of plasma renal (R) cortexin (PRC), a hypotensive protein produced in the kidney cortex cells, was reported to be reduced from 218 nM in the plasma of normotensive persons (NP) to 0 nM in the plasma of patients with EH. The feasibility of using the determination of PRC by enzyme-linked immunosorbent assay (ELISA) as a diagnostic test for EH was investigated.
  
  
  METHODS
  The PRC was determined by ELISA using electrophoretically pure cortexin as the antigen. A total of 344 persons (male and female) with EH, with or without diabetes mellitus (DM), and receiving or not receiving any anti-hypertensive and/or anti-diabetic medication at presentation, as well as an equal number of age- and gender-matched NP participated in the study.
  
  
  RESULTS
  All persons with EH, with or without co-existing DM, were found to have 0 nM PRC, regardless of whether they were receiving anti-hypertensive and anti-diabetic drugs, including those who had been taking these medications over an extended period of time (3 months).
  
  
  CONCLUSIONS
  The determination of PRC as a marker protein by ELISA, a rapid method that can be carried out in any diagnostic laboratory, was shown to be suitable for the diagnosis of EH, even in those subjects who had co-existing DM and were receiving both anti-hypertensive and anti-diabetic medication.
authors.affiliation: Sinha Institute of Medical Science and Technology, Calcutta, India.
authors.forename: Rajeshwary
authors.initials: R
authors.lastname: Ghosh
authors.forename: Mau
authors.initials: M
authors.lastname: Bhattacharyya
authors.forename: Gausal
authors.initials: G
authors.lastname: Khan
authors.forename: Somashree
authors.initials: S
authors.lastname: Chakraborty
authors.forename: Rabindra
authors.initials: R
authors.lastname: Bhattacharya
authors.forename: Uttam K
authors.initials: UK
authors.lastname: Maji
authors.forename: Pradipta
authors.initials: P
authors.lastname: Jana
authors.forename: Asru K
authors.initials: AK
authors.lastname: Sinha
mesh_terms: D000328:Adult; D000959:Antihypertensive Agents / Q000627:therapeutic use; D015415:Biomarkers / Q000097:blood; D048909:Diabetes Complications / Q000097:blood / Q000188:drug therapy; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005260:Female; D006801:Humans; D006943:Hyperglycemia / Q000097:blood / Q000188:drug therapy; D006973:Hypertension / Q000097:blood* / Q000188:drug therapy; D007004:Hypoglycemic Agents / Q000627:therapeutic use; D036341:Intercellular Signaling Peptides and Proteins; D008297:Male; D010455:Peptides / Q000097:blood*; D018709:Statistics, Nonparametric
chemical_list: D000959:Antihypertensive Agents; D015415:Biomarkers; D007004:Hypoglycemic Agents; D036341:Intercellular Signaling Peptides and Proteins; D010455:Peptides; C056590:cortexin