title: Sphingobacterium changzhouense sp. nov., a bacterium isolated from a rice field.
abstract: A Gram-stain-negative, yellow, non-spore-forming, rod-shaped bacterium, designated N7(T), was isolated from a soil sample collected from a rice field in Jiangsu, China, and was characterized using a polyphasic taxonomic approach. Strain N7(T) grew optimally at 25-30 °C, pH 6.0-8.0, and in the presence of 1 % NaCl (w/v). 16S rRNA gene sequence analysis indicated that strain N7(T) was a member of the genus Sphingobacterium and was closely related to Sphingobacterium multivorum IAM14316(T) (97.49 %) and Sphingobacterium canadense CR11(T) (97.11 %), sharing less than 97 % sequence similarities with other species of the genus Sphingobacterium. The predominant respiratory quinone of strain N7(T) was MK-7 and major fatty acids were summed features 3 (C16 : 1ω6c and/or C16 : 1ω7c), iso-C15 : 0, C16 : 0 and iso-C17 : 0 3-OH. The G+C content of the DNA was 40.9±0.5 mol%. The levels of DNA-DNA relatedness between strain N7(T) and the most closely related species S. multivorum IAM 14316(T) and S. canadense CR11(T) were 21 % and 15 %, respectively. Based on these results, strain N7(T) is proposed to represent a separate species within the genus Sphingobacterium. The name Sphingobacterium changzhouense sp. nov. is suggested and the type strain is N7(T) ( = CCTCC AB 2012100(T) = KACC 16854(T)).
authors.affiliation: Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China.
authors.forename: Hongming
authors.initials: H
authors.lastname: Liu
authors.affiliation: College of Resources and Environmental Sciences, Nanjing Agricultural University, 210095, PR China.
authors.forename: Jun
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China.
authors.forename: Dingbin
authors.initials: D
authors.lastname: Chen
authors.affiliation: Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China.
authors.forename: Li
authors.initials: L
authors.lastname: Cao
authors.affiliation: Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China.
authors.forename: Peng
authors.initials: P
authors.lastname: Lu
authors.affiliation: Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China.
authors.forename: ZhiGuo
authors.initials: Z
authors.lastname: Wu
authors.affiliation: Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China.
authors.forename: Fan
authors.initials: F
authors.lastname: Yang
authors.affiliation: Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China.
authors.forename: Shunpeng
authors.initials: S
authors.lastname: Li
authors.affiliation: Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Hong
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D012275:Oryza / Q000382:microbiology*; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D045249:Sphingobacterium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C062629:menaquinone 7