title: Serotype-specific identification of Dengue virus by silicon nanowire array biosensor.
abstract: In this work, we demonstrated a silicon nanowire (SiNW) biosensing platform capable of simultaneously identifying different Dengue serotypes on a single sensing chip. Four peptide nucleic acids (PNAs), specific to each Dengue serotypes (DENV-1 to DENV-4), were spotted on different areas of the SiNW array surface, and the covalently immobilized PNA probes were then interacted with different Dengue serotypes target to establish the specificity of detection. Detection scheme is based on the changes in resistances due to accumulation of negative charges contributed by the hybridized DNA target. The results show that resistance changes only occur in regions where the Dengue target hybridizes with its complementary probe. What is more, a mixture of two different Dengue serotypes obtained from a one-step duplex RT-PCR was applied to the multiplex SiNW surface to validate SiNW capability to identify multiple Dengue serotypes on a single sensing platform. Through this study, we have established the multiplex SiNW biosensor as a promising device to detect multiple Dengue infections with high specificity.
authors.affiliation: Institute of Microelectronics, A* STAR (Agency for Science, Technology and Research), 11 Science Park Road, Singapore 117685, China.
authors.forename: Min Joon
authors.initials: MJ
authors.lastname: Huang
authors.forename: Hui
authors.initials: H
authors.lastname: Xie
authors.forename: Qiangqiang
authors.initials: Q
authors.lastname: Wan
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.forename: Yong
authors.initials: Y
authors.lastname: Ning
authors.forename: Guo-Jun
authors.initials: GJ
authors.lastname: Zhang
mesh_terms: D001483:Base Sequence; D015374:Biosensing Techniques; D017931:DNA Primers; D003716:Dengue Virus / Q000145:classification / Q000302:isolation & purification*; D053770:Nanowires; D016133:Polymerase Chain Reaction; D012825:Silicon
chemical_list: D017931:DNA Primers; D012825:Silicon