title: Obesity-related metabolomic analysis of human subjects in black soybean peptide intervention study by ultraperformance liquid chromatography and quadrupole-time-of-flight mass spectrometry.
abstract: The present study aimed to identify key metabolites related to weight reduction in humans by studying the metabolic profiles of sera obtained from 34 participants who underwent dietary intervention with black soybean peptides (BSP) for 12 weeks. This research is a sequel to our previous work in which the effects of BSP on BMI and blood composition of lipid were investigated. Sera of the study were subjected to ultra performance liquid chromatography and quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS), and the data were analyzed using partial least-squares discriminate analysis (PLS-DA) score plots. Body mass index and percent body fat of the test group were reduced. Levels of betaine, benzoic acid, pyroglutamic acid, pipecolic acid, N-phenylacetamide, uric acid, l-aspartyl-l-phenylalanine, and lysophosphatidyl cholines (lysoPCs) (C18:1, C18:2, C20:1, and C20:4) showed significant increases. Levels of l-proline, valine, l-leucine/isoleucine, hypoxanthine, glutamine, l-methionine, phenylpyruvic acid, several carnitine derivatives, and lysoPCs (C14:0, PC16:0, C15:0, C16:0, C17:1, C18:0, and C22:0) were significantly decreased. In particular, lysoPC 16:0 with a VIP value of 12.02 is esteemed to be the most important metabolite for evaluating the differences between the 2 serum samples. Our result confirmed weight-lowering effects of BSP, accompanied by favorable changes in metabolites in the subjects' blood. Therefore, this research enables us to better understand obesity and increases the predictability of the obesity-related risk by studying metabolites present in the blood.
authors.affiliation: Department of Food Metabolism and Nutrition, Korea Food Research Institute, 516 Paekhyon-dong, Pundang-ku, Songnam, Kyongki-do 463-747, Republic of Korea.
authors.forename: Min Jung
authors.initials: MJ
authors.lastname: Kim
authors.forename: Hye Jeong
authors.initials: HJ
authors.lastname: Yang
authors.forename: Jin Hee
authors.initials: JH
authors.lastname: Kim
authors.forename: Chang-Won
authors.initials: CW
authors.lastname: Ahn
authors.forename: Jong Ho
authors.initials: JH
authors.lastname: Lee
authors.forename: Kang Sung
authors.initials: KS
authors.lastname: Kim
authors.forename: Dae Young
authors.initials: DY
authors.lastname: Kwon
mesh_terms: D000328:Adult; D000368:Aged; D015415:Biomarkers / Q000097:blood; D002853:Chromatography, Liquid; D019587:Dietary Supplements; D016002:Discriminant Analysis; D005260:Female; D006801:Humans; D016018:Least-Squares Analysis; D008297:Male; D055432:Metabolomics / Q000379:methods*; D008875:Middle Aged; D009765:Obesity / Q000097:blood / Q000175:diagnosis / Q000188:drug therapy*; D014674:Plant Proteins, Dietary / Q000627:therapeutic use*; D013025:Glycine max; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013997:Time Factors; D016896:Treatment Outcome; D015431:Weight Loss / Q000187:drug effects; D055815:Young Adult
chemical_list: D015415:Biomarkers; D014674:Plant Proteins, Dietary