title: Development and evaluation of monoclonal antibodies for the glucoside of T-2 toxin (t2-glc).
abstract: The interactions between fungi and plants can yield metabolites that are toxic in animal systems. Certain fungi are known to produce sesquiterpenoid trichothecenes, such as T-2 toxin, that are biotransformed by several mechanisms including glucosylation. The glucosylated forms have been found in grain and are of interest as potential reservoirs of T-2 toxin that are not detected by many analytical methods. Hence the glucosides of trichothecenes are often termed "masked" mycotoxins. The glucoside of T-2 toxin (T2-Glc) was linked to keyhole limpet hemocyanin and used to produce antibodies in mice. Ten monoclonal antibody (Mab)-producing hybridoma cell lines were developed. The Mabs were used in immunoassays to detect T2-Glc and T-2 toxin, with midpoints of inhibition curves (IC50s) in the low ng/mL range. Most of the Mabs demonstrated good cross-reactivity to T-2 toxin, with lower recognition of HT-2 toxin. One of the clones (2-13) was further characterized with in-depth cross-reactivity and solvent tolerance studies. Results suggest Mab 2-13 will be useful for the simultaneous detection of T-2 toxin and T2-Glc.
authors.affiliation: Bacterial Foodborne Pathogens and Mycology Research Unit, USDA-ARS-NCAUR, 1815 N. University St., Peoria, IL 61604, USA. chris.maragos@ars.usda.gov
authors.forename: Chris M
authors.initials: CM
authors.lastname: Maragos
authors.forename: Cletus
authors.initials: C
authors.lastname: Kurtzman
authors.forename: Mark
authors.initials: M
authors.lastname: Busman
authors.forename: Neil
authors.initials: N
authors.lastname: Price
authors.forename: Susan
authors.initials: S
authors.lastname: McCormick
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D002460:Cell Line; D002851:Chromatography, High Pressure Liquid; D003429:Cross Reactions; D002523:Edible Grain / Q000737:chemistry / Q000382:microbiology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005960:Glucosides / Q000378:metabolism*; D006825:Hybridomas / Q000166:cytology / Q000378:metabolism; D007114:Immunization; D020128:Inhibitory Concentration 50; D051379:Mice; D008807:Mice, Inbred BALB C; D013605:T-2 Toxin / Q000031:analogs & derivatives* / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D005960:Glucosides; D013605:T-2 Toxin