title: Ethanol fermentation from xylose by metabolically engineered strains of Kluyveromyces marxianus.
abstract: We constructed a xylose-fermenting recombinant strain of thermotolerant yeast Kluyveromyces marxianus, DMB3-7. Both xylose consumption and ethanol production were remarkably increased in DMB3-7 compared to the control strain at 30°C. Furthermore, DMB3-7 produced ethanol from xylose at both 42°C and 45°C, above which xylose metabolic activity decreased.
authors.affiliation: Biomass Refinery Research Center (BRRC), National Institute of Advanced Industrial Science and Technology (AIST), 3-11-32 Kagamiyama, Higashi-hiroshima, Hiroshima 739-0046, Japan.
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Goshima
authors.forename: Kanako
authors.initials: K
authors.lastname: Negi
authors.forename: Masaharu
authors.initials: M
authors.lastname: Tsuji
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Inoue
authors.forename: Shinichi
authors.initials: S
authors.lastname: Yano
authors.forename: Tamotsu
authors.initials: T
authors.lastname: Hoshino
authors.forename: Akinori
authors.initials: A
authors.lastname: Matsushika
mesh_terms: D000449:Aldehyde Reductase / Q000378:metabolism; D056804:Biofuels / Q000600:supply & distribution; D050542:D-Xylulose Reductase / Q000378:metabolism; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation; D007716:Kluyveromyces / Q000235:genetics* / Q000378:metabolism*; D060847:Metabolic Engineering; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000378:metabolism; D013696:Temperature; D014994:Xylose / Q000378:metabolism*
chemical_list: D056804:Biofuels; D000431:Ethanol; D014994:Xylose; D000449:Aldehyde Reductase; D050542:D-Xylulose Reductase; D017853:Phosphotransferases (Alcohol Group Acceptor); C032700:xylulokinase
keywords: Bioethanol; Thermotolerant yeast; Xylitol dehydrogenase; Xylose; Xylose reductase; Xylulokinase