title: Prolonged sleep fragmentation of mice exacerbates febrile responses to lipopolysaccharide.
abstract: BACKGROUND
  Sleep disruption is a frequent occurrence in modern society. Whereas many studies have focused on the consequences of total sleep deprivation, few have investigated the condition of sleep disruption.
  
  
  METHODS
  We disrupted sleep of mice during the light period for 9 consecutive days using an intermittently rotating disc.
  
  
  RESULTS
  Electroencephalogram (EEG) data demonstrated that non-rapid eye movement (NREM) sleep was severely fragmented and REM sleep was essentially abolished during the 12h light period. During the dark period, when sleep was not disrupted, neither NREM sleep nor REM sleep times differed from control values. Analysis of the EEG revealed a trend for increased power in the peak frequency of the NREM EEG spectra during the dark period. The fragmentation protocol was not overly stressful as body weights and water consumption remained unchanged, and plasma corticosterone did not differ between mice subjected to 3 or 9 days of sleep disruption and home cage controls. However, mice subjected to 9 days of sleep disruption by this method responded to lipopolysaccharide with an exacerbated febrile response.
  
  
  METHODS
  Existing methods to disrupt sleep of laboratory rodents often subject the animal to excessive locomotion, vibration, or sudden movements. This method does not suffer from any of these confounds.
  
  
  CONCLUSIONS
  This study demonstrates that prolonged sleep disruption of mice exacerbates febrile responses to lipopolysaccharide. This device provides a method to determine mechanisms by which chronic insufficient sleep contributes to the etiology of many pathologies, particularly those with an inflammatory component.
authors.affiliation: Department of Anesthesiology & Pain Medicine, University of Washington, Seattle, WA, USA.
authors.forename: Kristyn M
authors.initials: KM
authors.lastname: Ringgold
authors.forename: R Paulien
authors.initials: RP
authors.lastname: Barf
authors.forename: Amrita
authors.initials: A
authors.lastname: George
authors.forename: Blair C
authors.initials: BC
authors.lastname: Sutton
authors.forename: Mark R
authors.initials: MR
authors.lastname: Opp
mesh_terms: D000818:Animals; D001522:Behavior, Animal; D001835:Body Weight / Q000187:drug effects; D002908:Chronic Disease; D003345:Corticosterone / Q000097:blood; D003627:Data Interpretation, Statistical; D004326:Drinking / Q000187:drug effects; D004569:Electroencephalography / Q000187:drug effects; D005334:Fever / Q000139:chemically induced* / Q000503:physiopathology*; D007274:Injections, Intraperitoneal; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D012890:Sleep / Q000187:drug effects / Q000502:physiology; D012893:Sleep Wake Disorders / Q000139:chemically induced* / Q000503:physiopathology*; D012895:Sleep, REM / Q000187:drug effects / Q000502:physiology
chemical_list: D008070:Lipopolysaccharides; D003345:Corticosterone
keywords: Fever; Inflammation; LPS; Rodent; Stress