title: Relative contribution of biomolecules in bacterial extracellular polymeric substances to disinfection byproduct formation.
abstract: In this study, detailed chemical compositions of the biomolecules in extracellular polymeric substances (EPS) from both pure cultures of bacteria and mixed species biofilm isolated from a water utility were analyzed. Then, based on detailed EPS analysis results, the DBP yield experiments were conducted with both extracted EPS and surrogate chemicals to indirectly identify the influence of biomolecules and their structures on DBP formation and speciation. DBP yield results of both extracted EPS and EPS surrogates indicated that proteins in EPS have a greater influence on DBP formation, especially on the formation of nitrogenous DBPs (N-DBPs), where amino acids containing unsaturated organic carbon or conjugated bonds in R-group produced higher amount of DBPs. For regulated DBPs, HAA yields were higher than THM yields, while haloacetonitriles were the dominant DBP species formed among unregulated DBPs. However, DBP yields of polysaccharide monomers were lower than those of tested amino acids groups and the DBP yields of polysaccharide monomers were not significantly influenced by their structures. Considering the results obtained in this study, biofilm needs to be considered an important precursor to DBP formation and biofilm eradication methods for water distribution systems need to be carefully selected to minimize subsequent DBP formation.
authors.affiliation: Department of Chemical and Environmental Engineering, University of Toledo , Mail Stop 307, 3048 Nitschke Hall, Toledo, Ohio 43606, United States.
authors.forename: Zhikang
authors.initials: Z
authors.lastname: Wang
authors.forename: Onekyun
authors.initials: O
authors.lastname: Choi
authors.forename: Youngwoo
authors.initials: Y
authors.lastname: Seo
mesh_terms: D001419:Bacteria / Q000254:growth & development; D018407:Bacterial Physiological Phenomena / Q000187:drug effects*; D001426:Bacterial Proteins / Q000378:metabolism; D018441:Biofilms / Q000187:drug effects* / Q000254:growth & development; D002851:Chromatography, High Pressure Liquid; D015169:Colony Count, Microbial; D004202:Disinfectants / Q000378:metabolism*; D060766:Drinking Water / Q000382:microbiology; D050356:Lipid Metabolism; D011108:Polymers / Q000378:metabolism; D011135:Polysaccharides, Bacterial / Q000378:metabolism; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000254:growth & development; D016958:Pseudomonas putida / Q000187:drug effects / Q000254:growth & development; D014874:Water Pollutants, Chemical / Q000378:metabolism*; D018508:Water Purification
chemical_list: D001426:Bacterial Proteins; D004202:Disinfectants; D060766:Drinking Water; D011108:Polymers; D011135:Polysaccharides, Bacterial; D014874:Water Pollutants, Chemical