title: Oral administration of poly-γ-glutamic acid prevents the development of atopic dermatitis in NC/Nga mice.
abstract: Bacillus subtilis-derived poly-γ-glutamic acid (γPGA) has demonstrated adjuvant activity in promoting Th1/Th17 cell differentiation. Here, the NC/Nga (NC) mouse model was used to determine whether γPGA modulates the outcome of atopic dermatitis (AD), which is known to be a Th2-biased immune disease. We found that oral administration of γPGA dramatically reduced the development of AD in NC mice. Antigen-presenting cells activated with γPGA produced pro-inflammatory cytokines, such as IL12/23 and IFNγ, which, in turn, induced the differentiation of Th1 and Th17 cells. Concomitantly, Th2 responses, such as high levels of serum IgE, were dramatically decreased. Furthermore, in vivo γPGA treatment altered several cellular components of allergic reactions, such as mast cells and eosinophils. Taken together, our results strongly demonstrate that in vivo treatment with γPGA at early time points can prevent the development of AD in NC mice and suggest that γPGA may have therapeutic applications for human AD.
authors.affiliation: Department of Bioscience and Biotechnology, Institute of Bioscience, Sejong University, Seoul, Korea.
authors.forename: Sung Won
authors.initials: SW
authors.lastname: Lee
authors.forename: Hyun Jung
authors.initials: HJ
authors.lastname: Park
authors.forename: Se-Ho
authors.initials: SH
authors.lastname: Park
authors.forename: Seokmann
authors.initials: S
authors.lastname: Hong
mesh_terms: D000284:Administration, Oral; D000818:Animals; D000938:Antigen-Presenting Cells / Q000166:cytology; D017252:CD3 Complex / Q000378:metabolism; D002454:Cell Differentiation; D016207:Cytokines / Q000378:metabolism; D003876:Dermatitis, Atopic / Q000517:prevention & control*; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D004804:Eosinophils / Q000166:cytology; D005786:Gene Expression Regulation; D007073:Immunoglobulin E / Q000737:chemistry; D007249:Inflammation; D008297:Male; D008407:Mast Cells / Q000166:cytology; D051379:Mice; D011099:Polyglutamic Acid / Q000008:administration & dosage / Q000031:analogs & derivatives*; D018417:Th1 Cells / Q000166:cytology; D058504:Th17 Cells / Q000166:cytology
chemical_list: D017252:CD3 Complex; D016207:Cytokines; C511775:poly(gamma-glutamic acid); D011099:Polyglutamic Acid; D007073:Immunoglobulin E
keywords: T helper differentiation; atopic dermatitis; cytokine; mast cell; poly-γ-glutamic acid