title: Female SHR have greater blood pressure sensitivity and renal T cell infiltration following chronic NOS inhibition than males.
abstract: Nitric oxide is a critical regulator of blood pressure (BP) and inflammation, and female spontaneously hypertensive rats (SHR) have higher renal nitric oxide bioavailability than males. We hypothesize that female SHR will have a greater rise in BP and renal T cell infiltration in response to nitric oxide synthase (NOS) inhibition than males. Both male and female SHR displayed a dose-dependent increase in BP to the nonspecific NOS inhibitor N(G)-nitro-L-arginine methyl ester (L-NAME: 2, 5, and 7 mg·kg(-1)·day(-1) for 4 days each); however, females exhibited a greater increase in BP than males. Treatment of male and female SHR with 7 mg·kg(-1)·day(-1) L-NAME for 2 wk significantly increased BP in both sexes; however, prior exposure to L-NAME only increased BP sensitivity to chronic NOS inhibition in females. L-NAME-induced hypertension increased renal T cell infiltration and indices of renal injury in both sexes, yet female SHR exhibited greater increases in Th17 cells and greater decreases in regulatory T cells than males. Chronic L-NAME was also associated with larger increases in renal cortical adhesion molecule expression in female SHR. The use of triple therapy to block L-NAME-mediated increases in BP attenuated L-NAME-induced increases in renal T cell counts and normalized adhesion molecule expression in SHR, suggesting that L-NAME-induced increases in renal T cells were dependent on both increases in BP and NOS inhibition. Our data suggest that NOS is critical in the ability of SHR, females in particular, to maintain BP and limit a pro-inflammatory renal T cell profile.
authors.affiliation: Departments of Medicine.
authors.forename: Krystal N
authors.initials: KN
authors.lastname: Brinson
authors.forename: Ahmed A
authors.initials: AA
authors.lastname: Elmarakby
authors.forename: Ashlee J
authors.initials: AJ
authors.lastname: Tipton
authors.forename: G Ryan
authors.initials: GR
authors.lastname: Crislip
authors.forename: Tatsuo
authors.initials: T
authors.lastname: Yamamoto
authors.forename: Babak
authors.initials: B
authors.lastname: Baban
authors.forename: Jennifer C
authors.initials: JC
authors.lastname: Sullivan
mesh_terms: D000818:Animals; D000959:Antihypertensive Agents / Q000494:pharmacology; D001794:Blood Pressure / Q000187:drug effects*; D015815:Cell Adhesion Molecules / Q000378:metabolism; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D004359:Drug Therapy, Combination; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D005260:Female; D006973:Hypertension / Q000188:drug therapy / Q000201:enzymology* / Q000276:immunology / Q000473:pathology / Q000503:physiopathology; D007249:Inflammation / Q000201:enzymology* / Q000276:immunology / Q000473:pathology / Q000503:physiopathology / Q000517:prevention & control; D007668:Kidney / Q000187:drug effects* / Q000276:immunology / Q000473:pathology / Q000503:physiopathology; D008297:Male; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology*; D009569:Nitric Oxide / Q000378:metabolism; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors* / Q000378:metabolism; D051381:Rats; D011918:Rats, Inbred SHR; D012737:Sex Factors; D013601:T-Lymphocytes / Q000187:drug effects* / Q000276:immunology; D013997:Time Factors
chemical_list: D000959:Antihypertensive Agents; D015815:Cell Adhesion Molecules; D004791:Enzyme Inhibitors; D009569:Nitric Oxide; D019001:Nitric Oxide Synthase; D019331:NG-Nitroarginine Methyl Ester
keywords: hypertension; inflammation; kidney injury; nitric oxide synthase; sex