title: Development of sample preparation method for eleutheroside B and E analysis in Acanthopanax senticosus by ionic liquids-ultrasound based extraction and high-performance liquid chromatography detection.
abstract: An ionic liquids-based ultrasonic-assisted extraction (ILUAE) method was successfully developed for extracting eleutheroside B and E from Radix Acanthopanax senticosus. Thirteen 1-alkyl-3-methylimidazolium ionic liquids with different cations and anions were investigated and 1-butyl-3-methylimidazolium bromide ([C4mim]Br) solution was selected as the solvent. The conditions for ILUAE, including the ionic liquid concentration, soaking time, ultrasonic power, ultrasonic time, solid-liquid ratio and number of extraction cycles, were optimized. With the proposed method, the energy consumption time was reduced to 30 min, whereas conventional method requires about 4h. The proposed method had good recovery (97.96-103.39%) and reproducibility (RSD, n=5; 3.3% for eleutheroside B, 4.6% for eleutheroside E). ILUAE was an efficient, rapid and simple sample preparation technique that showed high reproducibility and was environmental friendly.
authors.affiliation: Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, 150040 Harbin, China. ylnefu@163.com
authors.forename: Lei
authors.initials: L
authors.lastname: Yang
authors.forename: Hongshuang
authors.initials: H
authors.lastname: Ge
authors.forename: Wenjie
authors.initials: W
authors.lastname: Wang
authors.forename: Yuangang
authors.initials: Y
authors.lastname: Zu
authors.forename: Fengjian
authors.initials: F
authors.lastname: Yang
authors.forename: Chunjian
authors.initials: C
authors.lastname: Zhao
authors.forename: Lin
authors.initials: L
authors.lastname: Zhang
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D005591:Chemical Fractionation / Q000295:instrumentation / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D028001:Eleutherococcus / Q000737:chemistry*; D005960:Glucosides / Q000032:analysis / Q000302:isolation & purification*; D052578:Ionic Liquids / Q000737:chemistry; D017705:Lignans / Q000032:analysis / Q000302:isolation & purification*; D010666:Phenylpropionates / Q000032:analysis / Q000302:isolation & purification*; D010936:Plant Extracts / Q000032:analysis / Q000302:isolation & purification*; D014465:Ultrasonics / Q000379:methods*
chemical_list: D005960:Glucosides; D052578:Ionic Liquids; D017705:Lignans; D010666:Phenylpropionates; D010936:Plant Extracts; C421885:eleutheroside E; C028305:syringin
keywords: Acanthopanax senticosus; Eleutheroside B; Eleutheroside E; Ionic liquid; Response surface methodology; Ultrasonic-assisted extraction