title: Immunological detection of large oxidized lipoproteins in hypertriglyceridemic serum.
abstract: BACKGROUND
  Triglyceride-rich, low-density lipoproteins (TG-rich LDL) have been reported as an oxidized lipoprotein species in patients with severe liver disease. Using TG-rich LDL as an immunogen, we obtained a monoclonal antibody (G11-6) that reacted with TG-rich LDL from patients with liver disease and with metal-oxidized LDL only in the early process of the oxidation reaction. This study determined the G11-6-reactive lipoproteins in hypertriglyceridemic serum.
  
  
  METHODS
  Serum samples from healthy volunteers (n = 12) and hypertriglyceridemic patients (n = 9) were fractionated by gel filtration and subjected to a sandwich enzyme-linked immunosorbent assay (ELISA) using G11-6 and polyclonal anti-apolipoprotein B antibodies.
  
  
  RESULTS
  Small LDL and larger lipoproteins reacted with G11-6. G11-6-reactive small LDL was identified in both the healthy subjects and hypertriglyceridemic patients, whereas G11-6-reactive larger lipoproteins were found only in the hypertriglyceridemic patients.
  
  
  CONCLUSIONS
  G11-6 is a useful tool for detecting increased large oxidized lipoproteins in hypertriglyceridemic patients.
authors.affiliation: Faculty of Health Sciences, Hokkaido University, Sapporo, Japan.
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Sakurai
authors.forename: Norio
authors.initials: N
authors.lastname: Wada
authors.forename: Yuji
authors.initials: Y
authors.lastname: Takahashi
authors.forename: Ayako
authors.initials: A
authors.lastname: Ichikawa
authors.forename: Akiko
authors.initials: A
authors.lastname: Ikuta
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Furumaki
authors.forename: Shu-Ping
authors.initials: SP
authors.lastname: Hui
authors.forename: Shigeki
authors.initials: S
authors.lastname: Jin
authors.forename: Seiji
authors.initials: S
authors.lastname: Takeda
authors.forename: Hirotoshi
authors.initials: H
authors.lastname: Fuda
authors.forename: Masato
authors.initials: M
authors.lastname: Fujikawa
authors.forename: Chikara
authors.initials: C
authors.lastname: Shimizu
authors.forename: Hironori
authors.initials: H
authors.lastname: Nagasaka
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Furukawa
authors.forename: Seiichi
authors.initials: S
authors.lastname: Kobayashi
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Chiba
mesh_terms: D000328:Adult; D000368:Aged; D000911:Antibodies, Monoclonal; D001055:Apolipoproteins B / Q000097:blood / Q000276:immunology; D016022:Case-Control Studies; D002850:Chromatography, Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D015228:Hypertriglyceridemia / Q000097:blood* / Q000175:diagnosis; D008077:Lipoproteins, LDL / Q000097:blood*; D008297:Male; D008875:Middle Aged; D010084:Oxidation-Reduction; D014280:Triglycerides / Q000097:blood*
chemical_list: D000911:Antibodies, Monoclonal; D001055:Apolipoproteins B; D008077:Lipoproteins, LDL; D014280:Triglycerides; C032607:low density lipoprotein triglyceride
keywords: Lipids; analytes; immunoassay; laboratory methods