title: Effect of dietary mineral sources and oil content on calcium utilization and kidney calcification in female Fischer rats fed low-protein diets.
abstract: We studied the effects of dietary mineral source and oil intake on kidney calcification in 4-wk-old female Fischer rats after consuming the AIN-76 purified diet (AIN-76). A modified AIN-76 mineral mixture was used, although the original calcium (Ca)/phosphorus (P) molar ratio remained unchanged. Rats were fed the modified diets for a period of 40 d before their kidneys were removed on the last day. Ca balance tests were performed on days 31 to 36 and biochemical analysis of urine was also studied. Kidney Ca, P, and magnesium (Mg) in the standard diet group (20% protein and 5% oil) were not affected by the mineral source. Kidney Ca, P, and Mg in the low-protein (10% protein) diet group, were found to be influenced by the dietary oil content and mineral source. In particular, the different mineral sources differentially increased kidney mineral accumulation. Pathological examination of the kidney showed that the degree of kidney calcification was proportional to the dietary oil content in the 10% dietary protein group, reflecting the calcium content of the kidney. The information gathered on mineral sources in this study will help future researchers studying the influence of dietary Ca/P molar ratios, and histological changes in the kidney.
authors.affiliation: Department of Administrative Nutrition, Faculty of Health and Nutrition, Tokyo Seiei College, Japan. otsuka@tsc-05.ac.jp
authors.forename: Shizuko
authors.initials: S
authors.lastname: Ohtsuka
authors.forename: Yoshiko
authors.initials: Y
authors.lastname: Aoyama
authors.forename: Nobuhiro
authors.initials: N
authors.lastname: Watanabe
authors.forename: Tomoko
authors.initials: T
authors.lastname: Kajiwara
authors.forename: Shoji
authors.initials: S
authors.lastname: Azami
authors.forename: Takao
authors.initials: T
authors.lastname: Kitano
mesh_terms: D000818:Animals; D002114:Calcinosis / Q000139:chemically induced* / Q000378:metabolism / Q000473:pathology / Q000652:urine; D019355:Calcium Citrate / Q000378:metabolism / Q000494:pharmacology / Q000652:urine; D002130:Calcium Phosphates / Q000378:metabolism / Q000494:pharmacology / Q000652:urine; D002136:Calcium, Dietary / Q000008:administration & dosage* / Q000378:metabolism / Q000494:pharmacology / Q000652:urine; D004032:Diet; D004044:Dietary Proteins / Q000008:administration & dosage; D005260:Female; D007668:Kidney / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D008274:Magnesium / Q000378:metabolism / Q000652:urine; D008903:Minerals / Q000008:administration & dosage / Q000378:metabolism / Q000652:urine; D010710:Phosphates / Q000008:administration & dosage / Q000378:metabolism / Q000494:pharmacology / Q000652:urine; D016226:Phosphorus, Dietary / Q000008:administration & dosage* / Q000378:metabolism / Q000494:pharmacology / Q000652:urine; D017680:Potassium Compounds / Q000378:metabolism / Q000494:pharmacology / Q000652:urine; D051381:Rats; D011916:Rats, Inbred F344; D013024:Soybean Oil / Q000008:administration & dosage* / Q000494:pharmacology
chemical_list: D002130:Calcium Phosphates; D002136:Calcium, Dietary; D004044:Dietary Proteins; D008903:Minerals; D010710:Phosphates; D016226:Phosphorus, Dietary; D017680:Potassium Compounds; D013024:Soybean Oil; C020243:calcium phosphate; C013216:potassium phosphate; D008274:Magnesium; D019355:Calcium Citrate