title: Peel LTP (Pru p 3)--the major allergen of peach--is methylated. A proteomic study.
abstract: Lipid transfer protein (LTP, Pru p 3) is the major allergen of peach (Prunus persica), and is in a greater abundance in the peel than in the pulp of the fruit. Peel LTP is more allergenic than pulp LTP, but it is not clear whether this is due to its specific allergenic properties or to its higher concentration. In this study, we have used a new one-step, rapid procedure for the purification of LTP from peel and pulp of four peach varieties [Gladys (white flesh), California (nectarine yellow flesh), Plusplus (yellow flesh), Red Fair (nectarine yellow flesh)] harvested in a field grown in Southern Italy. Purification was based on miniature reversed-phase chromatography, a procedure suitable for proteomic study. Proteomic analysis of purified LTPs revealed that the amino acid sequence of LTP was identical in all peach genotypes but, for the first time, peel LTP was found to be methylated.
authors.affiliation: Department of Sciences, University of Basilicata, 85100 Potenza, Italy.
authors.forename: Marilena
authors.initials: M
authors.lastname: Larocca
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Martelli
authors.forename: Gerarda
authors.initials: G
authors.lastname: Grossi
authors.forename: Maria Carmela
authors.initials: MC
authors.lastname: Padula
authors.forename: Paolo
authors.initials: P
authors.lastname: Riccio
authors.forename: Rocco
authors.initials: R
authors.lastname: Rossano
mesh_terms: D000485:Allergens / Q000737:chemistry / Q000378:metabolism*; D052179:Antigens, Plant / Q000737:chemistry* / Q000378:metabolism*; D005638:Fruit / Q000737:chemistry / Q000378:metabolism; D013058:Mass Spectrometry; D008745:Methylation; D010940:Plant Proteins / Q000737:chemistry* / Q000378:metabolism*; D040901:Proteomics; D027861:Prunus / Q000737:chemistry / Q000378:metabolism*
chemical_list: D000485:Allergens; D052179:Antigens, Plant; D010940:Plant Proteins; C463181:Pru p 3 allergen
keywords: Allergens; LTP; MALDI-TOF; Methylation; Protein purification; Reversed-phase chromatography