title: Antiglycation activity of thiamin-HCl and benfotiamine in diabetic condition.
abstract: OBJECTIVE
  To observe the antiglycation effect of water-soluble vitamin, thiamin-hydrochloride (B1) and its fat-soluble derivative benfotiamine and their comparison with two different glycation assays in diabetics.
  
  
  METHODS
  Plasma of both the normal's and diabetic persons was taken for this experimental study which was conducted in the Department of Chemistry and Biochemistry, University of Agriculture, Faisalabad in 2008. Varying concentrations of both the glycation inhibitors and glucose were incubated for 5 weeks at 37 degrees C. Thiobarbituric acid and periodate borohydride assays were used to measure the antiglycation activity.
  
  
  RESULTS
  The increase in glycation was observed from 1st to 3rd week of incubation, while it decreased after the 5th week due to the formation of advanced glycation end products.
  
  
  CONCLUSIONS
  It was observed that 10mM concentration of benfotiamine and 5mM and 1mM concentrations of thiamine-hydrochloride produced fairly good response to decreased glycation. Comparison between two assays proved periodate borohydride to be more reliable and sensitive than thiobarbituric acid.
authors.affiliation: Department of Biochemistry, University College of Medicine, University of Lahore, Pakistan. samphdbio@gmail.com
authors.forename: Samina
authors.initials: S
authors.lastname: Kousar
authors.forename: Munir Ahmad
authors.initials: MA
authors.lastname: Sheikh
authors.forename: Muhammad
authors.initials: M
authors.lastname: Asghar
mesh_terms: D001786:Blood Glucose / Q000187:drug effects* / Q000378:metabolism*; D002614:Chelating Agents / Q000494:pharmacology*; D003920:Diabetes Mellitus / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D017127:Glycation End Products, Advanced / Q000032:analysis; D006031:Glycosylation / Q000187:drug effects; D006801:Humans; D013831:Thiamine / Q000031:analogs & derivatives* / Q000494:pharmacology*; D014803:Vitamin B Complex / Q000494:pharmacology*
chemical_list: D001786:Blood Glucose; D002614:Chelating Agents; D017127:Glycation End Products, Advanced; D014803:Vitamin B Complex; D013831:Thiamine; C013835:benphothiamine