title: Sub-150 nm mesoporous silica nanoparticles with tunable pore sizes and well-ordered mesostructure for protein encapsulation.
abstract: Despite their great potentials as biomacromolecues delivery vehicles, there are few, if any, reports on mesoporous silica nanoparticles (MSNs) simultaneously integrated with the merits of large pore size, small particle diameters and well-ordered mesostructure. Here, we designed a facile strategy for the synthesis of monodispersed MSNs using cationic surfactants (CSs) as templating agents, neutral amine of N,N-dimethylhexadecylamine (DMHA) as a pore size mediator and tri-block copolymer of F127 (EO106PO70EO106) as a particle growth inhibitor/dispersant. The obtained colloidal nanoparticles exhibited a highly ordered mesostructure and tunable pore diameter up to 4.6 nm (BJH) and monodispersed particle sizes less than 150 nm. A model protein of cytochrome c (CytC) was exemplified to be accommodated in the resultant MSNs and its loading amount was correlated with their pore size. The efficient cancer cellular uptake of the large-pore MSNs prefigured their potentials as intracellular delivery vehicles for membrane-impermeable proteins.
authors.affiliation: Key Laboratory for Ultrafine Materials of Ministry of Education, School of Materials Science and Engineering, East China University of Science and Technology, Shanghai 200237, China. jinlougu@ecust.edu.cn
authors.forename: Jinlou
authors.initials: J
authors.lastname: Gu
authors.forename: Kai
authors.initials: K
authors.lastname: Huang
authors.forename: Xiangyang
authors.initials: X
authors.lastname: Zhu
authors.forename: Yongsheng
authors.initials: Y
authors.lastname: Li
authors.forename: Jie
authors.initials: J
authors.lastname: Wei
authors.forename: Wenru
authors.initials: W
authors.lastname: Zhao
authors.forename: Changsheng
authors.initials: C
authors.lastname: Liu
authors.forename: Jianlin
authors.initials: J
authors.lastname: Shi
mesh_terms: D005456:Fluorescent Dyes / Q000737:chemistry; D006367:HeLa Cells; D006801:Humans; D046529:Microscopy, Electron, Transmission; D015394:Molecular Structure; D053758:Nanoparticles / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry*; D053838:Scattering, Small Angle; D012822:Silicon Dioxide / Q000737:chemistry*; D014961:X-Ray Diffraction
chemical_list: D005456:Fluorescent Dyes; D011506:Proteins; D012822:Silicon Dioxide
keywords: Drug delivery; Mesoporous silica; Nanoparticles; Protein encapsulation; Tunable pore size