title: Imaging bacterial protein expression using genetically encoded RNA sensors.
abstract: The difficulties in imaging the dynamics of protein expression in live bacterial cells can be overcome by using fluorescent sensors based on Spinach, an RNA that activates the fluorescence of a small-molecule fluorophore. These RNAs selectively bind target proteins and exhibit fluorescence increases that enable protein expression to be imaged in living Escherichia coli. These sensors are key components of a generalizable strategy to image protein expression in a single bacterium in real time.
authors.affiliation: Department of Pharmacology, Weill Medical College, Cornell University, New York, New York, USA.
authors.forename: Wenjiao
authors.initials: W
authors.lastname: Song
authors.forename: Rita L
authors.initials: RL
authors.lastname: Strack
authors.forename: Samie R
authors.initials: SR
authors.lastname: Jaffrey
mesh_terms: D052157:Aptamers, Nucleotide / Q000235:genetics; D001426:Bacterial Proteins / Q000032:analysis* / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D001593:Benzyl Compounds / Q000737:chemistry; D015374:Biosensing Techniques / Q000379:methods*; D036022:Capsid Proteins / Q000032:analysis; D029968:Escherichia coli Proteins / Q000032:analysis / Q000378:metabolism; D005456:Fluorescent Dyes / Q000737:chemistry / Q000378:metabolism; D048288:Imidazolines / Q000737:chemistry; D017909:Levivirus / Q000378:metabolism; D057054:Molecular Imaging / Q000379:methods*; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D061848:Optical Imaging / Q000379:methods; D015347:RNA Probes / Q000737:chemistry* / Q000235:genetics*; D019809:Streptavidin / Q000737:chemistry
chemical_list: C560120:3,5-difluoro-4-hydroxybenzylidene imidazolinone; D052157:Aptamers, Nucleotide; D001426:Bacterial Proteins; D001593:Benzyl Compounds; D036022:Capsid Proteins; D029968:Escherichia coli Proteins; D005456:Fluorescent Dyes; D048288:Imidazolines; D015347:RNA Probes; D019809:Streptavidin