title: miR-761 regulates the mitochondrial network by targeting mitochondrial fission factor.
abstract: Mitochondria are dynamic organelles that constantly undergo fission and fusion. The balance between fission and fusion determines the fate of the cell. In this study, we show that mitochondrial fission factor (MFF) is upregulated upon hydrogen peroxide treatment or ischemia/reperfusion (I/R) injury. Knockdown of MFF attenuated hydrogen peroxide- and I/R injury-induced cardiomyocyte apoptosis and myocardial infarction. We found that MFF is a direct target of miR-761, and miR-761 inhibits mitochondrial fission and cardiomyocyte apoptosis by repressing MFF. This study reveals a novel model of mitochondrial fission regulation, which is composed of miR-761 and MFF. Modulation of their levels may provide a new approach for tackling apoptosis and myocardial infarction.
authors.affiliation: Division of Cardiovascular Research, State Key Laboratory of Biomembrane and Membrane Biotechnology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Long
authors.affiliation: Division of Cardiovascular Research, State Key Laboratory of Biomembrane and Membrane Biotechnology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Kun
authors.initials: K
authors.lastname: Wang
authors.affiliation: Division of Cardiovascular Research, State Key Laboratory of Biomembrane and Membrane Biotechnology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Na
authors.initials: N
authors.lastname: Li
authors.affiliation: Division of Cardiovascular Research, State Key Laboratory of Biomembrane and Membrane Biotechnology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China; Signal Transduction Laboratory, Department of Biochemistry, Quaid-i-Azam University, 45320 Islamabad, Pakistan.
authors.forename: Iram
authors.initials: I
authors.lastname: Murtaza
authors.affiliation: Division of Cardiovascular Research, State Key Laboratory of Biomembrane and Membrane Biotechnology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Jing-Ying
authors.initials: JY
authors.lastname: Xiao
authors.affiliation: Division of Cardiovascular Research, State Key Laboratory of Biomembrane and Membrane Biotechnology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Yuan-Yuan
authors.initials: YY
authors.lastname: Fan
authors.affiliation: Division of Cardiovascular Research, State Key Laboratory of Biomembrane and Membrane Biotechnology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Cui-Yun
authors.initials: CY
authors.lastname: Liu
authors.affiliation: The second affiliated hospital, Hebei North University, Hebei 075100, China.
authors.forename: Wen-Hui
authors.initials: WH
authors.lastname: Li
authors.affiliation: The second affiliated hospital, Hebei North University, Hebei 075100, China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Cheng
authors.affiliation: Division of Cardiovascular Research, State Key Laboratory of Biomembrane and Membrane Biotechnology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China; College of Medicine, University of Illinois at Chicago, Chicago, IL 60612, USA. Electronic address: longbo@ioz.ac.cn.
authors.forename: PeiFeng
authors.initials: P
authors.lastname: Li
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D015151:Immunoblotting; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D035683:MicroRNAs / Q000378:metabolism*; D046529:Microscopy, Electron, Transmission; D063154:Mitochondrial Dynamics / Q000502:physiology*; D024101:Mitochondrial Proteins / Q000235:genetics / Q000378:metabolism*; D032383:Myocytes, Cardiac / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014162:Transfection
chemical_list: C000600165:MIRN761 microRNA, mouse; C000600166:MIRN761 microRNA, rat; D035683:MicroRNAs; D024101:Mitochondrial Proteins
keywords: Apoptosis; Free radicals; Hydrogen peroxide; Ischemia/reperfusion; MFF; MicroRNAs; Mitochondrial fission