title: Different toxicity of the novel Bacillus thuringiensis (Bacillales: Bacillaceae) strain LLP29 against Aedes albopictus and Culex quinquefasciatus (Diptera: Culicidae).
abstract: Bacillus thuringiensis (Bt) (Berliner) strain LLP29 produces a crystal protein Cyt1Aa6 toxic to mosquito vectors of human diseases. However, the susceptibility of Culex quinquefasciatus (Say) in the current study was 8.25 times higher than that of Aedes albopictus (Skuse) with this single protein Cyt1Aa6 purified from LLP29. To understand the mechanism of the novel mosquitocidal protein, the binding characteristic of brush border membrane vesicles from the two tested mosquitoes was investigated. Enzyme-linked immunosorbent assay showed that Cyt1Aa6 bound to the two mosquitoes' brush border membrane vesicles. However, the titer of Ae. albopictus was a little higher than that of Cx. quinquefasciatus, with 3.21 and 2.91, respectively. Ligand Western blot analysis showed Cyt1Aa6 toxin specifically bound to the same three proteins (i.e., 68, 54, and 26 kDa) in the two mosquitoes, but one another protein, approximately to 37 kDa, could just be detected in Cx. quinquefasciatus. However, little difference was found in the test of immunohistochemistry. Cyt1Aa6 was detected in the midguts of both mosquitoes with histopathological changes. It would of great importance to the knowledge of the novel toxin against to Cx. quinquefasciatus and Ae. albopictus.
authors.affiliation: Key Laboratory of Biopesticide and Chemical Biology, Ministry of Education, Fujian Agriculture and Forestry University, 350002 Fuzhou, Fujian, People's Republic of China.
authors.forename: Lingling
authors.initials: L
authors.lastname: Zhang
authors.forename: Baozhen
authors.initials: B
authors.lastname: Tang
authors.forename: Enjiong
authors.initials: E
authors.lastname: Huang
authors.forename: Zhipeng
authors.initials: Z
authors.lastname: Huang
authors.forename: Zhaoxia
authors.initials: Z
authors.lastname: Liu
authors.forename: Tianpei
authors.initials: T
authors.lastname: Huang
authors.forename: Ivan
authors.initials: I
authors.lastname: Gelbic
authors.forename: Xiong
authors.initials: X
authors.lastname: Guan
authors.forename: Lei
authors.initials: L
authors.lastname: Xu
mesh_terms: D000330:Aedes / Q000187:drug effects*; D000818:Animals; D001413:Bacillus thuringiensis / Q000235:genetics / Q000472:pathogenicity*; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000494:pharmacology*; D003465:Culex / Q000187:drug effects*; D004591:Electrophoresis, Polyacrylamide Gel; D004731:Endotoxins / Q000494:pharmacology*; D004797:Enzyme-Linked Immunosorbent Assay; D006460:Hemolysin Proteins / Q000494:pharmacology*; D007150:Immunohistochemistry; D009032:Mosquito Control; D010572:Pest Control, Biological
chemical_list: D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D004731:Endotoxins; D006460:Hemolysin Proteins; C030956:insecticidal crystal protein, Bacillus Thuringiensis