title: MYBL2 is a sub-haploinsufficient tumor suppressor gene in myeloid malignancy.
abstract: A common deleted region (CDR) in both myelodysplastic syndromes (MDS) and myeloproliferative neoplasms (MPN) affects the long arm of chromosome 20 and has been predicted to harbor a tumor suppressor gene. Here we show that MYBL2, a gene within the 20q CDR, is expressed at sharply reduced levels in CD34+ cells from most MDS cases (65%; n = 26), whether or not they harbor 20q abnormalities. In a murine competitive reconstitution model, Mybl2 knockdown by RNAi to 20-30% of normal levels in multipotent hematopoietic progenitors resulted in clonal dominance of these 'sub-haploinsufficient' cells, which was reflected in all blood cell lineages. By 6 months post-transplantation, the reconstituted mice had developed a clonal myeloproliferative/myelodysplastic disorder originating from the cells with aberrantly reduced Mybl2 expression. We conclude that downregulation of MYBL2 activity below levels predicted by classical haploinsufficiency underlies the clonal expansion of hematopoietic progenitors in a large fraction of human myeloid malignancies. DOI:http://dx.doi.org/10.7554/eLife.00825.001.
authors.affiliation: Institute of Transfusion Medicine , University Hospital Essen , Essen , Germany ; Department of Pediatric Oncology , Dana-Farber Cancer Institute , Boston , United States.
authors.forename: Stefan
authors.initials: S
authors.lastname: Heinrichs
authors.forename: Lillian F
authors.initials: LF
authors.lastname: Conover
authors.forename: Carlos E
authors.initials: CE
authors.lastname: Bueso-Ramos
authors.forename: Outi
authors.initials: O
authors.lastname: Kilpivaara
authors.forename: Kristen
authors.initials: K
authors.lastname: Stevenson
authors.forename: Donna
authors.initials: D
authors.lastname: Neuberg
authors.forename: Mignon L
authors.initials: ML
authors.lastname: Loh
authors.forename: Wen-Shu
authors.initials: WS
authors.lastname: Wu
authors.forename: Scott J
authors.initials: SJ
authors.lastname: Rodig
authors.forename: Guillermo
authors.initials: G
authors.lastname: Garcia-Manero
authors.forename: Hagop M
authors.initials: HM
authors.lastname: Kantarjian
authors.forename: A Thomas
authors.initials: AT
authors.lastname: Look
mesh_terms: D018797:Cell Cycle Proteins / Q000235:genetics*; D004303:Dosage Compensation, Genetic; D016147:Genes, Tumor Suppressor; D057895:Haploinsufficiency / Q000235:genetics*; D006801:Humans; D007621:Karyotyping; D009190:Myelodysplastic Syndromes / Q000235:genetics*; D009196:Myeloproliferative Disorders / Q000235:genetics*; D015534:Trans-Activators / Q000235:genetics*
chemical_list: D018797:Cell Cycle Proteins; C064352:MYBL2 protein, human; D015534:Trans-Activators
keywords: 20q CDR; Human; MYBL2; Mouse; Myelodysplastic Syndromes