title: Determination of photocyanine in human serum by HPLC and application to pharmacokinetic study.
abstract: Photocyanine, a novel amphoteric phthalocyanine drug, showed favorable anticancer activity in vivo. Pharmacokinetic study in cancer patients is an important component in dose administration choice. In this study, a rapid, sensitive analytical method based on high-performance liquid chromatography with ultraviolet detection was developed and validated for the determination of four isomers of photocyanine (FD1-4) in cancer patients. Sample preparation involved liquid-liquid extraction with a combination of ultrasound and N,N-dimethyl formamide. Calibration curves (1/x(2)) offered satisfactory linearity for the four isomers of photocyanine. The lower limit of quantification (LLOQ) for FD1-3 isomers was 30 ng/mL, and LLOQ for FD-4 was 5 ng/mL. Inter- and intra-day accuracies for four isomers ranged from 96.6 to 105.5%, and 95.0 to 103.6%, respectively. Inter- and intra-day precision ranged from 4.8 to 8.9%, and 3.6 to 12.2%, respectively. Stability studies showed that photocyanine was stable. This method was successfully used to quantify photocyanine in a pharmacokinetic study in which a single-dose of photocyanine (0.1 mg/kg) was intravenously administered to patients with cancer. On the basis of the discovery that photocyanine has a half-life of 57.5 h in vivo, we suggest that avoiding light for a longer period is essential for patients undergoing photocyanine therapy.
authors.affiliation: State Key Laboratory of Oncology in Southern China, Sun Yat-sen University, Guangzhou, Guangdong, PR China Department of Clinical Trial Center, Cancer Center, Sun Yat-sen University, Guangzhou, Guangdong, PR China lisu@sysucc.org.cn lisusu@126.com.
authors.forename: Su
authors.initials: S
authors.lastname: Li
authors.affiliation: Department of Pharmacy, Guangzhou Eighth People's Hospital, Guangdong 510000, PR China.
authors.forename: Feng-Bi
authors.initials: FB
authors.lastname: Jian
authors.affiliation: State Key Laboratory of Oncology in Southern China, Sun Yat-sen University, Guangzhou, Guangdong, PR China Department of Clinical Trial Center, Cancer Center, Sun Yat-sen University, Guangzhou, Guangdong, PR China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhan
authors.affiliation: State Key Laboratory of Oncology in Southern China, Sun Yat-sen University, Guangzhou, Guangdong, PR China Department of Clinical Trial Center, Cancer Center, Sun Yat-sen University, Guangzhou, Guangdong, PR China.
authors.forename: Ben-Yan
authors.initials: BY
authors.lastname: Zou
authors.affiliation: State Key Laboratory of Oncology in Southern China, Sun Yat-sen University, Guangzhou, Guangdong, PR China Department of Clinical Trial Center, Cancer Center, Sun Yat-sen University, Guangzhou, Guangdong, PR China.
authors.forename: Hai
authors.initials: H
authors.lastname: Liao
mesh_terms: D000328:Adult; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005260:Female; D006801:Humans; D007211:Indoles / Q000097:blood* / Q000493:pharmacokinetics*; D054833:Isoindoles; D008297:Male; D009369:Neoplasms / Q000097:blood / Q000188:drug therapy; D015203:Reproducibility of Results
chemical_list: D007211:Indoles; D054833:Isoindoles; C013647:phthalocyanine