title: Pig transgenesis by piggyBac transposition in combination with somatic cell nuclear transfer.
abstract: The production of animals by somatic cell nuclear transfer (SCNT) is inefficient, with approximately 2% of micromanipulated oocytes going to term and resulting in live births. However, it is the most commonly used method for the generation of cloned transgenic livestock as it facilitates the attainment of transgenic animals once the nuclear donor cells are stably transfected and more importantly as alternatives methods of transgenesis in farm animals have proven even less efficient. Here we describe piggyBac-mediated transposition of a transgene into porcine primary cells and use of these genetically modified cells as nuclear donors for the generation of transgenic pigs by SCNT. Gene transfer by piggyBac transposition serves to provide an alternative approach for the transfection of nuclear donor cells used in SCNT.
authors.affiliation: Department of Animal Genetics, Breeding and Reproduction, South China Agricultural University, The New Building of College of Animal Science, Room 315, Guangzhou, 510642, Guangdong, People's Republic of China.
authors.forename: Zhenfang
authors.initials: Z
authors.lastname: Wu
authors.forename: Zhiqian
authors.initials: Z
authors.lastname: Xu
authors.forename: Xian
authors.initials: X
authors.lastname: Zou
authors.forename: Fang
authors.initials: F
authors.lastname: Zeng
authors.forename: Junsong
authors.initials: J
authors.lastname: Shi
authors.forename: Dewu
authors.initials: D
authors.lastname: Liu
authors.forename: Johann
authors.initials: J
authors.lastname: Urschitz
authors.forename: Stefan
authors.initials: S
authors.lastname: Moisyadi
authors.forename: Zicong
authors.initials: Z
authors.lastname: Li
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified / Q000235:genetics*; D004251:DNA Transposable Elements / Q000235:genetics*; D005347:Fibroblasts / Q000166:cytology; D018014:Gene Transfer Techniques; D049452:Green Fluorescent Proteins / Q000235:genetics; D053652:Nuclear Transfer Techniques; D009865:Oocytes / Q000166:cytology / Q000254:growth & development; D013552:Swine / Q000235:genetics*; D019076:Transgenes
chemical_list: D004251:DNA Transposable Elements; D049452:Green Fluorescent Proteins