title: Hsa-miR-181a-5p expression and effects on cell proliferation in gastric cancer.
abstract: OBJECTIVE
  MicroRNAs (miRNAs) are small endogenous, non-coding, single-stranded RNAs (approximately 22 nt). Accumulating evidence has shown that aberrant miRNA expression is pronounced and correlated with gastric cancer genesis and progression.
  
  
  METHODS
  Expression levels of miR-181a-5p in GC tissues and cell lines were assessed by qRT-PCR and tested for correlation with clinical features. In addition, effects of miR-181a-5p on GC cell growth were investigated.
  
  
  RESULTS
  Our findings indicate that miR-181a-5p is upregulated in GC, in correlation with lymph node invasion, nerve invasion and vascular invasion (P<0.05). Enforced expression of miR-181a -5p promoted cell proliferation ability.
  
  
  CONCLUSIONS
  This study suggested that increased miR-181a-5p is related to GC progression. MiR-181a-5p may represent a potential therapeutic target for GC.
authors.affiliation: Department of Oncology, the Affiliated Jiangning Hospital of Nanjing Medical University, Nanjing, China.
authors.forename: Gang
authors.initials: G
authors.lastname: Chen
authors.forename: Zhi-Li
authors.initials: ZL
authors.lastname: Shen
authors.forename: Ling
authors.initials: L
authors.lastname: Wang
authors.forename: Chun-Ye
authors.initials: CY
authors.lastname: Lv
authors.forename: Xin-En
authors.initials: XE
authors.lastname: Huang
authors.forename: Rong-Ping
authors.initials: RP
authors.lastname: Zhou
mesh_terms: D000236:Adenoma / Q000235:genetics / Q000473:pathology*; D014408:Biomarkers, Tumor / Q000235:genetics*; D049109:Cell Proliferation; D002478:Cells, Cultured; D005753:Gastric Mucosa / Q000378:metabolism*; D020869:Gene Expression Profiling; D006801:Humans; D008207:Lymphatic Metastasis; D035683:MicroRNAs / Q000235:genetics*; D009361:Neoplasm Invasiveness; D009367:Neoplasm Staging; D020411:Oligonucleotide Array Sequence Analysis; D011379:Prognosis; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013270:Stomach / Q000473:pathology; D013274:Stomach Neoplasms / Q000235:genetics / Q000473:pathology*
chemical_list: D014408:Biomarkers, Tumor; C540193:MIrn181 microRNA, human; D035683:MicroRNAs; D012333:RNA, Messenger