title: Differences in gene expression and cytokine levels between newly diagnosed and chronic pediatric ITP.
abstract: Immune thrombocytopenia (ITP) is an autoimmune disease where platelets are destroyed prematurely. In the majority of children the disease resolves, but in some it becomes chronic. To investigate whether these 2 phases of the disease are molecularly similar or separate entities we performed DNA microarray analysis (GEO accession number: GSE46922) of T-cells from newly diagnosed children and children with chronic ITP. We found complete separation of the gene expression profiles between the 2 phases of the disease. Furthermore, the gene expression levels of several cytokines differed between the 2 phases of the disease. This was also reflected in plasma with increased levels of interleukin (IL)-16 and TNF-related weak inducer of apoptosis and lower levels of IL-4 in newly diagnosed compared with chronic ITP. Thus, our data indicate that chronic ITP in childhood is a separate disease entity, dissimilar in many aspects to the newly diagnosed phase.
authors.affiliation: Department of Internal Medicine, University of Gothenburg, Gothenburg, Sweden.
authors.forename: Margareta
authors.initials: M
authors.lastname: Jernås
authors.forename: Yu
authors.initials: Y
authors.lastname: Hou
authors.forename: Frida
authors.initials: F
authors.lastname: Strömberg Célind
authors.forename: Linlin
authors.initials: L
authors.lastname: Shao
authors.forename: Intawat
authors.initials: I
authors.lastname: Nookaew
authors.forename: Qian
authors.initials: Q
authors.lastname: Wang
authors.forename: Xiuli
authors.initials: X
authors.lastname: Ju
authors.forename: Karin
authors.initials: K
authors.lastname: Mellgren
authors.forename: Hans
authors.initials: H
authors.lastname: Wadenvik
authors.forename: Ming
authors.initials: M
authors.lastname: Hou
authors.forename: Bob
authors.initials: B
authors.lastname: Olsson
mesh_terms: D002648:Child; D002908:Chronic Disease; D016000:Cluster Analysis; D016207:Cytokines / Q000097:blood* / Q000235:genetics*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D015894:Genome, Human / Q000235:genetics; D006801:Humans; D016553:Purpura, Thrombocytopenic, Idiopathic / Q000097:blood* / Q000175:diagnosis / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
chemical_list: D016207:Cytokines; D012333:RNA, Messenger