title: Nourseothricin N-acetyl transferase: a positive selection marker for mammalian cells.
abstract: Development of Nourseothricin N-acetyl transferase (NAT) as a selection marker for mammalian cells is described. Mammalian cells are acutely susceptible to Nourseothricin, similar to the widely used drug Puromycin, and NAT allows for quick and robust selection of transfected/transduced cells in the presence of Nourseothricin. NAT is compatible with other selection markers puromycin, hygromycin, neomycin, blasticidin, and is a valuable addition to the repertoire of mammalian selection markers.
authors.affiliation: Department of Cancer Biology, Dana-Farber Cancer Institute; Boston, Massachusetts, United States of America.
authors.forename: Bose S
authors.initials: BS
authors.lastname: Kochupurakkal
authors.forename: J Dirk
authors.initials: JD
authors.lastname: Iglehart
mesh_terms: D000123:Acetyltransferases / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D015415:Biomarkers / Q000378:metabolism; D002460:Cell Line; D002934:Cinnamates / Q000494:pharmacology; D006801:Humans; D006921:Hygromycin B / Q000031:analogs & derivatives / Q000494:pharmacology; D009355:Neomycin / Q000494:pharmacology; D011691:Puromycin / Q000494:pharmacology; D011760:Pyrrolidinones / Q000494:pharmacology; D013309:Streptothricins / Q000494:pharmacology*; D014162:Transfection / Q000379:methods*
chemical_list: D000900:Anti-Bacterial Agents; D015415:Biomarkers; D002934:Cinnamates; D011760:Pyrrolidinones; C420726:blasticidin A; D006921:Hygromycin B; C026273:hygromycin A; D011691:Puromycin; D013309:Streptothricins; D000123:Acetyltransferases; C050473:nourseothricin acetyltransferase; D009355:Neomycin