title: Cytotoxicity of nickel zinc ferrite nanoparticles on cancer cells of epithelial origin.
abstract: In this study, in vitro cytotoxicity of nickel zinc (NiZn) ferrite nanoparticles against human colon cancer HT29, breast cancer MCF7, and liver cancer HepG2 cells was examined. The morphology, homogeneity, and elemental composition of NiZn ferrite nanoparticles were investigated by scanning electron microscopy, transmission electron microscopy, and energy dispersive X-ray spectroscopy, respectively. The exposure of cancer cells to NiZn ferrite nanoparticles (15.6-1,000 μg/mL; 72 hours) has resulted in a dose-dependent inhibition of cell growth determined by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. The quantification of caspase-3 and -9 activities and DNA fragmentation to assess the cell death pathway of the treated cells showed that both were stimulated when exposed to NiZn ferrite nanoparticles. Light microscopy examination of the cells exposed to NiZn ferrite nanoparticles demonstrated significant changes in cellular morphology. The HepG2 cells were most prone to apoptosis among the three cells lines examined, as the result of treatment with NiZn nanoparticles. In conclusion, NiZn ferrite nanoparticles are suggested to have potential cytotoxicity against cancer cells.
authors.affiliation: Institute of Bioscience, Universiti Putra Malaysia, Selangor, Malaysia.
authors.forename: Mothanna Sadiq
authors.initials: MS
authors.lastname: Al-Qubaisi
authors.forename: Abdullah
authors.initials: A
authors.lastname: Rasedee
authors.forename: Moayad Husein
authors.initials: MH
authors.lastname: Flaifel
authors.forename: Sahrim H J
authors.initials: SH
authors.lastname: Ahmad
authors.forename: Samer
authors.initials: S
authors.lastname: Hussein-Al-Ali
authors.forename: Mohd Zobir
authors.initials: MZ
authors.lastname: Hussein
authors.forename: Eltayeb E M
authors.initials: EE
authors.lastname: Eid
authors.forename: Zulkarnain
authors.initials: Z
authors.lastname: Zainal
authors.forename: Mohd
authors.initials: M
authors.lastname: Saeed
authors.forename: Muna
authors.initials: M
authors.lastname: Ilowefah
authors.forename: Sharida
authors.initials: S
authors.lastname: Fakurazi
authors.forename: Norhaszalina
authors.initials: N
authors.lastname: Mohd Isa
authors.forename: Mohamed Ezzat
authors.initials: ME
authors.lastname: El Zowalaty
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology*; D053148:Caspase 3 / Q000378:metabolism; D053453:Caspase 9 / Q000378:metabolism; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects*; D053938:DNA Fragmentation / Q000187:drug effects; D004337:Drug Carriers / Q000737:chemistry*; D005290:Ferric Compounds / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry*; D009532:Nickel / Q000737:chemistry / Q000494:pharmacology*; D010316:Particle Size; D013055:Spectrophotometry, Infrared; D018709:Statistics, Nonparametric; D014961:X-Ray Diffraction; D017967:Zinc Compounds / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D000970:Antineoplastic Agents; D004337:Drug Carriers; D005290:Ferric Compounds; D017967:Zinc Compounds; C042584:nickel-zinc ferrite; D009532:Nickel; D053148:Caspase 3; D053453:Caspase 9
keywords: NiZn ferrite nanoparticles; anticancer; apoptosis; cancer cells lines