title: Isoliquiritigen enhances the antitumour activity and decreases the genotoxic effect of cyclophosphamide.
abstract: The aim of this study was to evaluate the antitumour activities and genotoxic effects of isoliquiritigenin (ISL) combined with cyclophosphamide (CP) in vitro and in vivo. U14 cells were treated with either of ISL (5-25 μg/mL) or CP (0.25-1.25 mg/mL) alone or with combination of ISL (5-25 μg/mL) and CP (1.0 mg/mL) for 48 h. The proliferation inhibitory effect in vitro was evaluated by MTT and colony formation assays. KM mice bearing U14 mouse cervical cancer cells were used to estimate the antitumour activity in vivo. The genotoxic activity in bone marrow polychromatic erythrocytes was assayed by frequency of micronuclei. The DNA damage in peripheral white blood cells was assayed by single cell gel electrophoresis. The results showed that ISL enhanced antitumour activity of CP in vitro and in vivo, and decreased the micronucleus formation in polychromatic erythrocytes and DNA strand breaks in white blood cells in a dose-dependent way.
authors.affiliation: School of Pharmacy, Shihezi University, Shihezi 832002, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Zhao
authors.forename: Xuan
authors.initials: X
authors.lastname: Yuan
authors.forename: Defang
authors.initials: D
authors.lastname: Li
authors.forename: Hongmei
authors.initials: H
authors.lastname: Chen
authors.forename: Jiangtao
authors.initials: J
authors.lastname: Jiang
authors.forename: Zhiping
authors.initials: Z
authors.lastname: Wang
authors.forename: Xiling
authors.initials: X
authors.lastname: Sun
authors.forename: Qiusheng
authors.initials: Q
authors.lastname: Zheng
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage* / Q000737:chemistry; D001854:Bone Marrow Cells / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects*; D047188:Chalcones / Q000008:administration & dosage* / Q000737:chemistry; D003520:Cyclophosphamide / Q000037:antagonists & inhibitors / Q000494:pharmacology / Q000633:toxicity; D004249:DNA Damage / Q000187:drug effects; D004912:Erythrocytes / Q000187:drug effects; D005260:Female; D006801:Humans; D051379:Mice; D002583:Uterine Cervical Neoplasms / Q000188:drug therapy / Q000473:pathology; D023041:Xenograft Model Antitumor Assays
chemical_list: D000970:Antineoplastic Agents; D047188:Chalcones; D003520:Cyclophosphamide; C040920:isoliquiritigenin