title: Differential expression of cathepsins K, S and V between young and aged Caucasian women skin epidermis.
abstract: Cutaneous aging translates drastic structural and functional alterations in the extracellular matrix (ECM). Multiple mechanisms are involved, including changes in protease levels. We investigated the age-related protein expression and activity of cysteine cathepsins and the expression of two endogenous protein inhibitors in young and aged Caucasian women skin epidermis. Immunofluorescence studies indicate that the expression of cathepsins K, S and V, as well as cystatins A and M/E within keratinocytes is reduced in photoprotected skin of aged women. Furthermore, the overall endopeptidase activity of cysteine cathepsins in epidermis lysates decreased with age. Albeit dermal elastic fiber and laminin expression is reduced in aged skin, staining of nidogen-1, a key protein in BM assembly that is sensitive to proteolysis by cysteine, metallo- and serine proteases, has a similar pattern in both young and aged skin. Since cathepsins contribute to the hydrolysis and turnover of ECM/basement membrane components, the abnormal protein degradation and deposition during aging process may be related in part to a decline of lysosomal/endosomal cathepsin K, S and V activity.
authors.affiliation: INSERM, UMR 1100, Pathologies Respiratoires: protéolyse et aérosolthérapie, Centre d'Etude des Pathologies Respiratoires, Université François Rabelais, F-37032 Tours cedex, France; LVMH-Recherche, BP58, F-45800 Saint Jean de Braye, France.
authors.forename: Juliette
authors.initials: J
authors.lastname: Sage
authors.affiliation: LVMH-Recherche, BP58, F-45800 Saint Jean de Braye, France.
authors.forename: Delphine
authors.initials: D
authors.lastname: De Quéral
authors.affiliation: LVMH-Recherche, BP58, F-45800 Saint Jean de Braye, France.
authors.forename: Emmanuelle
authors.initials: E
authors.lastname: Leblanc-Noblesse
authors.affiliation: LVMH-Recherche, BP58, F-45800 Saint Jean de Braye, France.
authors.forename: Robin
authors.initials: R
authors.lastname: Kurfurst
authors.affiliation: LVMH-Recherche, BP58, F-45800 Saint Jean de Braye, France.
authors.forename: Sylvianne
authors.initials: S
authors.lastname: Schnebert
authors.affiliation: LVMH-Recherche, BP58, F-45800 Saint Jean de Braye, France.
authors.forename: Eric
authors.initials: E
authors.lastname: Perrier
authors.affiliation: LVMH-Recherche, BP58, F-45800 Saint Jean de Braye, France.
authors.forename: Carine
authors.initials: C
authors.lastname: Nizard
authors.affiliation: INSERM, UMR 1100, Pathologies Respiratoires: protéolyse et aérosolthérapie, Centre d'Etude des Pathologies Respiratoires, Université François Rabelais, F-37032 Tours cedex, France.
authors.forename: Gilles
authors.initials: G
authors.lastname: Lalmanach
authors.affiliation: INSERM, UMR 1100, Pathologies Respiratoires: protéolyse et aérosolthérapie, Centre d'Etude des Pathologies Respiratoires, Université François Rabelais, F-37032 Tours cedex, France. Electronic address: fabien.lecaille@univ-tours.fr.
authors.forename: Fabien
authors.initials: F
authors.lastname: Lecaille
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D056657:Cathepsin K / Q000378:metabolism*; D002403:Cathepsins / Q000378:metabolism*; D015815:Cell Adhesion Molecules / Q000378:metabolism; D002478:Cells, Cultured; D015891:Cystatins / Q000378:metabolism; D003546:Cysteine Endopeptidases / Q000378:metabolism*; D004781:Environmental Exposure; D004817:Epidermis / Q000201:enzymology* / Q000502:physiology / Q000528:radiation effects; D005260:Female; D015870:Gene Expression; D006801:Humans; D015603:Keratinocytes / Q000201:enzymology; D008562:Membrane Glycoproteins / Q000378:metabolism; D008875:Middle Aged; D015595:Skin Aging / Q000528:radiation effects; D013472:Sunlight; D055815:Young Adult; D000097794:Kalinin
chemical_list: D056657:Cathepsin K; D002403:Cathepsins; D015815:Cell Adhesion Molecules; D015891:Cystatins; D003546:Cysteine Endopeptidases; D008562:Membrane Glycoproteins; D000097794:Kalinin; C029967:nidogen; C028217:cathepsin S; C534130:CTSK protein, human; C117627:CTSV protein, human
keywords: Aging; Cystatins; Cysteine proteases; Keratinocytes; Nidogen-1