title: Engineering bioconjugated gold nanospheres and gold nanorods as label-free plasmon scattering probes for ultrasensitive multiplex dark-field imaging of cancer cells.
abstract: In this paper, we proposed the use of gold nanoparticles as plasmon scattering probes for dark-field multiplex imaging of live cancer cells. By carefully engineering the surfaces, aqueous dispersions of anti-EGFR antibody-conjugated gold nanospheres and gold nanorods are prepared. We demonstrated the receptor-mediated delivery of antibody conjugated gold nanospheres and gold nanorods into EGFR receptor-positive oral squamous cell carcinoma cell line by using darkfield microscopy technique. Our result suggests that gold nanospheres and gold nanorods formulations could provide up to 7 types of plasmon scattering probes based on their tunability range with a 100 nm spectral separation in absorption bands.
authors.affiliation: Bio-Optical Imaging Group, Singapore Bioimaging Consortium (SBIC), Agency for Science Technology and Research (A *STAR), 11 Biopolis Way, 138667, Singapore.
authors.forename: Tianxun
authors.initials: T
authors.lastname: Gong
authors.forename: Malini
authors.initials: M
authors.lastname: Olivo
authors.forename: U S
authors.initials: US
authors.lastname: Dinish
authors.forename: Douglas
authors.initials: D
authors.lastname: Goh
authors.forename: Kien Voon
authors.initials: KV
authors.lastname: Kong
authors.forename: Ken-Tye
authors.initials: KT
authors.lastname: Yong
mesh_terms: D045744:Cell Line, Tumor; D003287:Contrast Media / Q000138:chemical synthesis; D006046:Gold; D006801:Humans; D007089:Image Enhancement / Q000379:methods; D008027:Light; D053768:Metal Nanoparticles / Q000737:chemistry; D008853:Microscopy / Q000379:methods*; D015336:Molecular Probe Techniques; D054118:Nanospheres / Q000737:chemistry; D009374:Neoplasms, Experimental / Q000473:pathology*; D015203:Reproducibility of Results; D012542:Scattering, Radiation; D012680:Sensitivity and Specificity; D013194:Staining and Labeling; D020349:Surface Plasmon Resonance / Q000379:methods*
chemical_list: D003287:Contrast Media; D006046:Gold