title: Cenderitide-eluting film for potential cardiac patch applications.
abstract: Cenderitide, also known as CD-NP, is a designer peptide developed by combining native mammalian c-type natriuretic peptide (CNP) and the C-terminus isolated from the dendroapis natriuretic peptide (DNP) of the venom from the green mamba. In early studies, intravenous and subcutaneous infusion of cenderitide was reported to reduce left ventricular (LV) mass and ameliorate cardiac remodelling. In this work, biodegradable polymeric films encapsulating CD-NP were developed and were investigated for their in vitro release and degradation characteristics. Subsequently, the bioactivity of released peptide and its effects on human cardiac fibroblast (HCF) were explored. We achieved sustained release from three films with low, intermediate and high release profiles for 30 days. Moreover, the bioactivity of released peptide was verified from the elevated production of cyclic guanosine monophospate (cGMP). The CD-NP released from films was able to inhibit the proliferation of hypertrophic HCF as well as suppress DNA synthesis in HCF. Furthermore, the sustained delivery from films showed comparable or superior suppressive actions on hypertrophic HCF compared to daily infusion of CD-NP. The results suggest that these films could be used to inhibit fibrosis and reduce cardiac remodelling via local delivery as cardiac patches.
authors.affiliation: School of Materials Science & Engineering, Nanyang Technological University, Singapore, Singapore.
authors.forename: Xu Wen
authors.initials: XW
authors.lastname: Ng
authors.forename: Yingying
authors.initials: Y
authors.lastname: Huang
authors.forename: Horng H
authors.initials: HH
authors.lastname: Chen
authors.forename: John C
authors.initials: JC
authors.lastname: Burnett
authors.forename: Freddy Y C
authors.initials: FY
authors.lastname: Boey
authors.forename: Subbu S
authors.initials: SS
authors.lastname: Venkatraman
mesh_terms: D002316:Cardiotonic Agents / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D006152:Cyclic GMP / Q000096:biosynthesis / Q000378:metabolism; D004247:DNA / Q000037:antagonists & inhibitors / Q000096:biosynthesis; D003692:Delayed-Action Preparations / Q000737:chemistry*; D004339:Drug Compounding; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D006801:Humans; D007700:Kinetics; D008752:Methylene Chloride / Q000737:chemistry; D045265:Natriuretic Peptides / Q000494:pharmacology*; D011091:Polyesters / Q000737:chemistry*; D012910:Snake Venoms / Q000494:pharmacology*; D057968:Transdermal Patch
chemical_list: D002316:Cardiotonic Agents; D003692:Delayed-Action Preparations; D045265:Natriuretic Peptides; D011091:Polyesters; D012910:Snake Venoms; C016240:polycaprolactone; D008752:Methylene Chloride; D004247:DNA; C575835:cenderitide; D006152:Cyclic GMP