title: [Effect of Ebosin on the inflammatory cytokines in THP-1 cells].
abstract: Ebosin is a novel exopolysaccharide produced by Streptomyces sp.139 with remarkable activity against rheumatic arthritis in vivo. In this paper, we reported effects of Ebosin on the inflammatory cytokines including interleukin-1beta (IL-1beta), interleukin-6 (IL-6) and tumor necrosis factor alpha (TNFalpha) in THP-1 cells. With the special fluorogenic peptide as substrates, the enzymatic activities of interleukin-1beta converting enzyme (ICE) and TNFalpha-converting enzyme (TACE) were inhibited by Ebosin separately. Using the real-time reverse transcription polymerase chain reaction (real-time PCR), the mRNA synthesis of the three cytokines were identified decline separately by Ebosin. The secretion quantum of three cytokines in THP-1 cells with Ebosin was lower than that of normal THP-1 cells determined by ELISA assay and Western blotting. All of these results showed that Ebosin has remarkably suppressed synthesis of the three cytokines in THP-1 cells through different pathways. The primary study of Ebosin on anti-inflammation mechanism was promoted developing the new drugs treating rheumatic arthritis.
authors.affiliation: Key Laboratory of Biotechnology of Antibiotics, Ministry of Health, Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhang
authors.forename: Lian-Hong
authors.initials: LH
authors.lastname: Guo
authors.forename: Yuan
authors.initials: Y
authors.lastname: Li
mesh_terms: D051722:ADAM Proteins / Q000378:metabolism*; D000072198:ADAM17 Protein; D000893:Anti-Inflammatory Agents / Q000494:pharmacology; D020170:Caspase 1 / Q000378:metabolism*; D045744:Cell Line, Tumor; D006801:Humans; D053583:Interleukin-1beta / Q000235:genetics / Q000378:metabolism*; D015850:Interleukin-6 / Q000235:genetics / Q000378:metabolism*; D007948:Leukemia, Monocytic, Acute / Q000378:metabolism* / Q000473:pathology; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis / Q000494:pharmacology*; D012333:RNA, Messenger / Q000378:metabolism; D013302:Streptomyces / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism*
chemical_list: D000893:Anti-Inflammatory Agents; C508600:IL6 protein, human; D053583:Interleukin-1beta; D015850:Interleukin-6; D011135:Polysaccharides, Bacterial; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; C000604294:ebosin; D020170:Caspase 1; D051722:ADAM Proteins; D000072198:ADAM17 Protein; C000606462:ADAM17 protein, human