title: DNA structures decorated with cathepsin G/secretory leukocyte proteinase inhibitor stimulate IFNI production by plasmacytoid dendritic cells.
abstract: Plasmacytoid dendritic cells (pDCs) and neutrophils are detected in psoriatic skin lesions and implicated in the pathogenesis of psoriasis. pDCs specialize in the production of type I interferon (IFNI), a cytokine that plays an important role in chronic autoimmune-like inflammation, including psoriasis. Here, we demonstrate that IFNI production in pDCs is stimulated by DNA structures containing the neutrophil serine protease cathepsin G (CatG) and the secretory leukocyte protease inhibitor (SLPI), which is a controlling inhibitor of serine proteases. We also demonstrate the presence of neutrophil-derived DNA structures containing CatG and SLPI in lesional skin samples from psoriasis patients. These findings suggest a previously unappreciated role for CatG in psoriasis by linking CatG and its inhibitor SLPI to the IFNI-dependent regulation of immune responses by pDCs in psoriatic skin.
authors.affiliation: Department of Immunology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University Kraków, Poland.
authors.forename: Joanna
authors.initials: J
authors.lastname: Skrzeczynska-Moncznik
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Wlodarczyk
authors.forename: Magdalena
authors.initials: M
authors.lastname: Banas
authors.forename: Mateusz
authors.initials: M
authors.lastname: Kwitniewski
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Zabieglo
authors.forename: Monika
authors.initials: M
authors.lastname: Kapinska-Mrowiecka
authors.forename: Adam
authors.initials: A
authors.lastname: Dubin
authors.forename: Joanna
authors.initials: J
authors.lastname: Cichy
keywords: Serine protease; autoimmunity; neutrophil extracellular traps; psoriasis