title: Rapid genotyping of three polymorphisms in the LBP gene using a triplex pyrosequencing approach.
abstract: We described a triplex polymerase chain reaction (PCR) and triplex pyrosequencing assay which allowed a simultaneous determination of three tag single nuleotide polymorphisms (tag SNPs) in the lipopolysaccharide-binding protein (LBP) gene: rs1780623, rs11536972 and rs2232618. This method enables a fast and cost-effective genotyping and a simultaneous determination of the three tag SNPs.
authors.affiliation: State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Surgery Research, Daping Hospital, Third Military Medical University, Chongqing, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Zeng
authors.forename: Jing
authors.initials: J
authors.lastname: Yu
authors.forename: Wei
authors.initials: W
authors.lastname: Gu
authors.forename: An-qiang
authors.initials: AQ
authors.lastname: Zhang
authors.forename: Cai-li
authors.initials: CL
authors.lastname: Yue
authors.forename: Ke-hong
authors.initials: KH
authors.lastname: Chen
authors.forename: Jian-xin
authors.initials: JX
authors.lastname: Jiang
mesh_terms: D000209:Acute-Phase Proteins / Q000235:genetics*; D002352:Carrier Proteins / Q000235:genetics*; D060005:Genotyping Techniques / Q000379:methods*; D006801:Humans; D008562:Membrane Glycoproteins / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D017422:Sequence Analysis, DNA / Q000379:methods*; D000099231:Lipopolysaccharide-Binding Protein
chemical_list: D000209:Acute-Phase Proteins; D002352:Carrier Proteins; D008562:Membrane Glycoproteins; D000099231:Lipopolysaccharide-Binding Protein
keywords: Lipopolysaccharide-Binding Protein; Single Nuleotide Polymorphism; Triplex pyrosequencing