title: Growth temperature-induced changes in biomass accumulation, photosynthesis and glutathione redox homeostasis as influenced by hydrogen peroxide in cucumber.
abstract: Hydrogen peroxide (H2O2) and glutathione (GSH) are involved in the stress response in plants. To elucidate the role of H2O2 in the acclimation of CO2 assimilation under sub- or supra-optimal growth temperatures, we examined the effect and interaction of H2O2 manipulation on the photosynthetic metabolism of cucumber plants (Cucumis sativus L.) grown under five temperature regimes spanning above and below the optimal growth temperature; 11/9, 18/15, 25/20, 32/27, and 39/33 °C (day/night), with or without dimethylthiourea (DMTU) or H2O2 treatment. As expected, exposure to sub- or supra-optimal growth temperatures resulted in decreased plant growth, associated with a decline in CO2 assimilation (Asat), Rubisco content, and activities of enzymes involved in the CO2 assimilation, as well as a decrease in the ratio of reduced (GSH) to oxidized (GSSG) glutathione (GSH/GSSG). Foliar application of H2O2 promoted, whilst DMTU retarded the capacity of plants to acclimate to non-optimal growth temperatures; this was consistently shown in altered activity of redox-sensitive enzymes involved in CO2 assimilation. These results strongly suggest that the influence of growth temperature on CO2 assimilation was primarily targeted at the activities of the redox-sensitive enzymes of CO2 assimilation. Meanwhile, the data suggest that the cellular H2O2 level is an important signal for the glutathione-dependent regulation of redox-sensitive enzymes of CO2 assimilation in cucumber plants.
authors.affiliation: Department of Horticulture, Zijingang Campus, Zhejiang University, Yuhangtang Road 866, Hangzhou 310058, PR China.
authors.forename: Hao
authors.initials: H
authors.lastname: Li
authors.forename: Xue Min
authors.initials: XM
authors.lastname: Wang
authors.forename: Li
authors.initials: L
authors.lastname: Chen
authors.forename: Golam Jalal
authors.initials: GJ
authors.lastname: Ahammed
authors.forename: Xiao Jian
authors.initials: XJ
authors.lastname: Xia
authors.forename: Kai
authors.initials: K
authors.lastname: Shi
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Considine
authors.forename: Jing Quan
authors.initials: JQ
authors.lastname: Yu
authors.forename: Yan Hong
authors.initials: YH
authors.lastname: Zhou
mesh_terms: D018533:Biomass; D018553:Cucumis sativus / Q000378:metabolism* / Q000502:physiology; D005978:Glutathione / Q000378:metabolism*; D006861:Hydrogen Peroxide / Q000378:metabolism*; D010084:Oxidation-Reduction; D010788:Photosynthesis / Q000502:physiology*; D013696:Temperature
chemical_list: D006861:Hydrogen Peroxide; D005978:Glutathione
keywords: 3-phosphoglycerate kinase; A(sat); Ci; Cucumis sativus; FBPase; GAPDH; GSH; GSSG; Gene transcript; H(2)O(2); J(max); PGK; PPFD; RCA; Redox; Rubisco; Rubisco activase; Suboptimal temperature; Supraoptimal temperature; V(cmax); fructose-1,6-bisphosphatase; glyceraldehyde-3-phosphate dehydrogenase; intercellular CO(2) concentration; oxidized glutathione; photosynthetic photo flux density; reduced glutathione; the light saturated rate of CO(2) assimilation; the maximum carboxylation rate of Rubisco; the maximum potential rate of electron transport contributed to ribulose-1,5-bisphosphate regeneration