title: Liposomal nanotransporter for targeted binding based on nucleic acid anchor system.
abstract: Microfluidic techniques have been developed intensively in recent years due to lower reagent consumption, faster analysis, and possibility of the integration of several analytical detectors into one chip. Electrochemical detectors are preferred in microfluidic systems, whereas liposomes can be used for amplification of the electrochemical signals. The aim of this study was to design a nanodevice for targeted anchoring of liposome as transport device. In this study, liposome with encapsulated Zn(II) was prepared. Further, gold nanoparticles were anchored onto the liposome surface allowing binding of thiol moiety-modified molecules (DNA). For targeted capturing of the transport device, DNA loops were used. DNA loops were represented by paramagnetic microparticles with oligo(DT)25 chain, on which a connecting DNA was bound. Capturing of transport device was subsequently done by hybridization to the loop. The individual steps were analyzed by electrochemistry and UV/Vis spectrometry. For detection of Zn(II) encapsulated in liposome, a microfluidic system was used. The study succeeded in demonstrating that liposome is suitable for the transport of Zn(II) and nucleic acids. Such transporter may be used for targeted binding using DNA anchor system.
authors.affiliation: Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Brno, Czech Republic.
authors.forename: Lukas
authors.initials: L
authors.lastname: Nejdl
authors.forename: Miguel Angel
authors.initials: MA
authors.lastname: Merlos Rodrigo
authors.forename: Jiri
authors.initials: J
authors.lastname: Kudr
authors.forename: Branislav
authors.initials: B
authors.lastname: Ruttkay-Nedecky
authors.forename: Marie
authors.initials: M
authors.lastname: Konecna
authors.forename: Pavel
authors.initials: P
authors.lastname: Kopel
authors.forename: Ondrej
authors.initials: O
authors.lastname: Zitka
authors.forename: Jaromir
authors.initials: J
authors.lastname: Hubalek
authors.forename: Rene
authors.initials: R
authors.lastname: Kizek
authors.forename: Vojtech
authors.initials: V
authors.lastname: Adam
mesh_terms: D006046:Gold / Q000737:chemistry; D008081:Liposomes / Q000737:chemistry* / Q000378:metabolism / Q000648:ultrastructure; D053768:Metal Nanoparticles / Q000737:chemistry; D046210:Microfluidic Analytical Techniques; D050997:Nanomedicine / Q000295:instrumentation; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D009696:Nucleic Acids / Q000737:chemistry* / Q000378:metabolism; D015032:Zinc / Q000737:chemistry
chemical_list: D008081:Liposomes; D009696:Nucleic Acids; D006046:Gold; D015032:Zinc
keywords: Gold nanoparticles; Liposome; Microfluidic; Nanomedicine; Nucleic acids; Paramagnetic particles