title: Combined intrathymic and intravenous injection of mesenchymal stem cells can prolong the survival of rat cardiac allograft associated with decrease in miR-155 expression.
abstract: BACKGROUND
  Mesenchymal stem cells (MSCs) have the potential to improve graft outcomes and promote allograft tolerance. In this study, we examined the effects and mechanism of combined intrathymic (i.t.) and intravenous (i.v.) injection of MSCs on the survival of transplanted hearts in a rat allograft model.
  
  
  METHODS
  Recipient Sprague-Dawley rats were transplanted with hearts from Wistar rats. Wistar rat MSCs were infused via i.t. or i.v. or combined i.t. and i.v. (i.t./i.v.) injection at designated intervals. In vitro mixed lymphocyte reaction assays were performed to assess the immunosuppressive capacity of MSCs. Mesenchymal stem cell surface markers and CD4+, CD25+, and Foxp3+ T-cells in the peripheral blood were detected using flow cytometry analysis. The expression of microRNAs and cytokines in graft infiltrating lymphocytes was analyzed by real-time polymerase chain reaction.
  
  
  RESULTS
  The MSCs cultured in vitro had multipotential differentiation capacity. Mixed lymphocyte reaction assays showed that donor-derived MSCs could not stimulate a proliferative response of recipient lymphocytes and could markedly suppress T-cell responses. Survival of the allografts was significantly prolonged by administration of i.t./i.v. injection of MSCs compared with controls, with a mean survival of 32.2 versus 6.5 d, respectively. Compared with the syngeneic groups posttransplant, miR-155 expression was significantly increased in the allogeneic group, and could be restored by injection of MSCs, especially i.t./i.v. injection of MSCs. Moreover, i.t./i.v. injection of MSCs decreased the level of interleukin (IL)-2 and interferon-gamma, but increased the levels of IL-4 and IL-10 in the allogeneic group. More important, i.t./i.v. injection of MSCs was the best way to increase the percentage of CD4+, CD25+, and Foxp3+ T-cell peripheral blood.
  
  
  CONCLUSIONS
  Our results indicated that i.t./i.v. injection of MSCs can prolong the survival of rat cardiac allograft, which may be associated with down-regulating miR-155 expression, a shift in the Th1/Th2 balance, and up-regulation of Treg cells expression.
authors.affiliation: Department of Cardiovascular Surgery, First Affiliated Hospital of Soochow University, Suzhou, China.
authors.forename: Haoyue
authors.initials: H
authors.lastname: Huang
authors.forename: Jigang
authors.initials: J
authors.lastname: He
authors.forename: Xiaomei
authors.initials: X
authors.lastname: Teng
authors.forename: Yunsheng
authors.initials: Y
authors.lastname: Yu
authors.forename: Wenxue
authors.initials: W
authors.lastname: Ye
authors.forename: Yanqiu
authors.initials: Y
authors.lastname: Hu
authors.forename: Zhenya
authors.initials: Z
authors.lastname: Shen
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D018920:Coculture Techniques; D006085:Graft Survival / Q000276:immunology*; D007275:Injections, Intravenous; D007959:Lymphocyte Culture Test, Mixed; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000276:immunology; D035683:MicroRNAs / Q000235:genetics*; D009206:Myocardium / Q000166:cytology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017208:Rats, Wistar; D050378:T-Lymphocytes, Regulatory / Q000166:cytology / Q000276:immunology; D018417:Th1 Cells / Q000166:cytology / Q000276:immunology; D018418:Th2 Cells / Q000166:cytology / Q000276:immunology; D013950:Thymus Gland / Q000166:cytology*; D014184:Transplantation, Homologous
chemical_list: C580597:MIRN155 microRNA, rat; D035683:MicroRNAs
keywords: Cytokine; IT/IV injection; MSCs; Rat cardiac allograft; miR-155