title: Simvastatin treatment increases nitrite levels in obese women: modulation by T(-786)C polymorphism of eNOS.
abstract: OBJECTIVE
  Evidence indicates an impairment of nitric oxide (NO) in obesity. Statins present pleiotropic effects independently of cholesterol-lowering, including increasing of eNOS expression and antioxidant effects. We evaluated the effects of simvastatin treatment at 45 days on circulating nitrite (NO marker) and TBARS-MDA levels in obese women without comorbidities (hypertension, diabetes and dyslipidemia). Moreover, we verified whether obese women carrying the C variant of T(-786)C polymorphism located in eNOS may have increased levels of nitrite after treatment compared to TT genotype.
  
  
  RESULTS
  After simvastatin treatment, while the plasma nitrite levels increased 42% (P=0.0008), the TBARS-MDA levels reduced 58% (P=0.0069). We observed increased levels of nitrite in both groups of genotypes (TT vs. TC+CC); however, rise in C-allele carriers was 60% comparing with 44% in TT.
  
  
  CONCLUSIONS
  Our results demonstrated a restoration of nitrite levels in obese women treated with simvastatin, which is modulated by T(-786)C polymorphism.
authors.affiliation: Núcleo de Pós-Graduação e Pesquisa - Santa Casa de Belo Horizonte, Rua Domingos Vieira 590, Belo Horizonte, MG, Brazil.
authors.forename: V L
authors.initials: VL
authors.lastname: Andrade
authors.forename: J T C
authors.initials: JT
authors.lastname: Sertório
authors.forename: N M
authors.initials: NM
authors.lastname: Eleuterio
authors.forename: J E
authors.initials: JE
authors.lastname: Tanus-Santos
authors.forename: K S
authors.initials: KS
authors.lastname: Fernandes
authors.forename: V C
authors.initials: VC
authors.lastname: Sandrim
mesh_terms: D000924:Anticholesteremic Agents / Q000627:therapeutic use; D005260:Female; D006801:Humans; D006937:Hypercholesterolemia / Q000188:drug therapy; D052250:Nitric Oxide Synthase Type III / Q000235:genetics* / Q000378:metabolism; D009573:Nitrites / Q000097:blood* / Q000378:metabolism; D009765:Obesity / Q000097:blood* / Q000188:drug therapy* / Q000201:enzymology / Q000235:genetics; D020641:Polymorphism, Single Nucleotide; D019821:Simvastatin / Q000627:therapeutic use*; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
chemical_list: D000924:Anticholesteremic Agents; D009573:Nitrites; D017392:Thiobarbituric Acid Reactive Substances; D019821:Simvastatin; C496314:NOS3 protein, human; D052250:Nitric Oxide Synthase Type III
keywords: Nitrite; Obesity; Polymorphism; Statin; eNOS