title: TIME FOR COFFEE is an essential component in the maintenance of metabolic homeostasis in Arabidopsis thaliana.
abstract: Plants often respond to environmental changes by reprogramming metabolic and stress-associated pathways. Homeostatic integration of signaling is a central requirement for ensuring metabolic stability in living organisms. Under diurnal conditions, properly timed rhythmic metabolism provides fitness benefits to plants. TIME FOR COFFEE (TIC) is a circadian regulator known to be involved in clock resetting at dawn. Here we explored the mechanism of influence of TIC in plant growth and development, as initiated by a microarray analysis. This global profiling showed that a loss of TIC function causes a major reprogramming of gene expression that predicts numerous developmental, metabolic, and stress-related phenotypes. This led us to demonstrate that this mutant exhibits late flowering, a plastochron defect, and diverse anatomical phenotypes. We further observed a starch-excess phenotype and altered soluble carbohydrate levels. tic exhibited hypersensitivity to oxidative stress and abscisic acid, and this was associated with a striking resistance to drought. These phenotypes were connected to an increase in total glutathione levels that correlated with a readjustment of amino acids and polyamine pools. By comparatively analyzing our transcriptomic and metabolomic data, we concluded that TIC is a central element in plant homeostasis that integrates and coordinates developmental, metabolic, and environmental signals.
authors.affiliation: Department of Plant Developmental Biology, Max Planck Institute for Plant Breeding Research, 50829, Cologne, Germany.
authors.forename: Alfredo
authors.initials: A
authors.lastname: Sanchez-Villarreal
authors.forename: Jieun
authors.initials: J
authors.lastname: Shin
authors.forename: Nora
authors.initials: N
authors.lastname: Bujdoso
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Obata
authors.forename: Ulla
authors.initials: U
authors.lastname: Neumann
authors.forename: Shen-Xiu
authors.initials: SX
authors.lastname: Du
authors.forename: Zhaojun
authors.initials: Z
authors.lastname: Ding
authors.forename: Amanda M
authors.initials: AM
authors.lastname: Davis
authors.forename: Takayuki
authors.initials: T
authors.lastname: Shindo
authors.forename: Elmon
authors.initials: E
authors.lastname: Schmelzer
authors.forename: Ronan
authors.initials: R
authors.lastname: Sulpice
authors.forename: Adriano
authors.initials: A
authors.lastname: Nunes-Nesi
authors.forename: Mark
authors.initials: M
authors.lastname: Stitt
authors.forename: Alisdair R
authors.initials: AR
authors.lastname: Fernie
authors.forename: Seth J
authors.initials: SJ
authors.lastname: Davis
mesh_terms: D000040:Abscisic Acid / Q000378:metabolism; D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000502:physiology*; D050260:Carbohydrate Metabolism; D002940:Circadian Rhythm / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D005978:Glutathione / Q000378:metabolism; D006706:Homeostasis; D055442:Metabolome; D009687:Nuclear Proteins / Q000235:genetics / Q000502:physiology*; D018384:Oxidative Stress; D010641:Phenotype; D013312:Stress, Physiological; D059467:Transcriptome
chemical_list: D029681:Arabidopsis Proteins; D009687:Nuclear Proteins; C523534:TIC protein, Arabidopsis; D000040:Abscisic Acid; D005978:Glutathione
keywords: Arabidopsis thaliana; TIME FOR COFFEE; circadian clock; metabolism; stress