title: Emergence of a multidrug-resistant (ASSuTTm) strain of Salmonella enterica serovar Typhimurium DT120 in England in 2011 and the use of multiple-locus variable-number tandem-repeat analysis in supporting outbreak investigations.
abstract: In summer 2011, two outbreaks of a unique, multidrug-resistant strain of Salmonella enterica serovar Typhimurium phage type 120 (DT120) occurred mainly in the Midlands, England. The first outbreak occurred among guests attending a wedding in July 2011 ('Wedding outbreak'), followed by a more geographically dispersed outbreak in August and September 2011 ('Midlands outbreak'). Fifty-one cases were confirmed. Detailed epidemiological and environmental health investigations suggested that pork was the most likely source of both outbreaks. All human samples and one pork sample showed the specific multiple-locus variable-number tandem-repeat analysis (MLVA) profile 3-11-12-NA-0211, with at most two loci variations. Trace-back investigations suggested a link to a butcher's shop and a pig farm in the East Midlands. The investigations highlight the utility of molecular analysis (MLVA) in supporting epidemiological investigations of outbreaks caused by S. Typhimurium DT120. Safe handling and cooking of pork by food business operators and consumers are key interventions to prevent future outbreaks.
authors.affiliation: 1 Public Health England , East Midlands, Leicester, United Kingdom .
authors.forename: Karthikeyan
authors.initials: K
authors.lastname: Paranthaman
authors.forename: Sophie
authors.initials: S
authors.lastname: Haroon
authors.forename: Samia
authors.initials: S
authors.lastname: Latif
authors.forename: Natalie
authors.initials: N
authors.lastname: Vinnyey
authors.forename: Valerie
authors.initials: V
authors.lastname: de Souza
authors.forename: William
authors.initials: W
authors.lastname: Welfare
authors.forename: Mamoona
authors.initials: M
authors.lastname: Tahir
authors.forename: Edward
authors.initials: E
authors.lastname: Cooke
authors.forename: Kirsten
authors.initials: K
authors.lastname: Stone
authors.forename: Chris
authors.initials: C
authors.lastname: Lane
authors.forename: Tansy
authors.initials: T
authors.lastname: Peters
authors.forename: Richard
authors.initials: R
authors.lastname: Puleston
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000818:Animals; D001434:Bacteriophage Typing; D002648:Child; D002675:Child, Preschool; D015331:Cohort Studies; D004196:Disease Outbreaks; D024901:Drug Resistance, Multiple, Bacterial; D004739:England / Q000453:epidemiology; D005260:Female; D005516:Food Microbiology; D005517:Foodborne Diseases / Q000453:epidemiology* / Q000382:microbiology; D005759:Gastroenteritis / Q000453:epidemiology* / Q000382:microbiology; D006801:Humans; D007223:Infant; D008297:Male; D008460:Meat / Q000382:microbiology*; D008875:Middle Aged; D018598:Minisatellite Repeats / Q000235:genetics; D012189:Retrospective Studies; D012480:Salmonella Infections / Q000453:epidemiology* / Q000382:microbiology; D012486:Salmonella typhimurium / Q000235:genetics / Q000302:isolation & purification*; D013552:Swine; D055815:Young Adult