title: Proopiomelanocortin gene polymorphisms and its association with meat quality traits by ultrasound measurement in Chinese cattle.
abstract: Ultrasound technology was used to measure live animal meat traits instead of true carcass meat traits for beef production and cattle breeding by an increasing number of institutions. In this study, we analyzed the association between genetic polymorphisms of proopiomelanocortin (POMC) and ultrasound measurement traits in Chinese cattle. Using direct DNA sequencing in 322 individuals of 7 different cattle subpopulation, 7 SNPs were identified for genotyping within 790bp region of intron 2 and exon 3 of POMC. 6586 T>G in intron 2 and 6769 C>T and 7216 C>T in exon 3 were significantly associated with ultrasound backfat thickness (UBF) (P<0.05) and ultrasound loin muscle area (ULMA) (P<0.01) in the total population; 6694 C>T, 6706 T>C, 6796 C>T and 6810 C>T in exon 3 were significantly associated with ULMA (P<0.0001) in the total population. These results clearly suggest that these SNPs of POMC be benefit for selection of individuals with good quality meat in Chinese cattle breeding program. Following validation in other populations and breeds, these markers could be incorporated into breeding programs to increase the rate of improvement in carcass and meat quality traits.
authors.affiliation: College of Food Engineering and Nutritional Science, Shaanxi Normal University, 710062, Xi'an, Shaanxi, PR China.
authors.forename: Yongfeng
authors.initials: Y
authors.lastname: Liu
authors.forename: Linsen
authors.initials: L
authors.lastname: Zan
authors.forename: Linqiang
authors.initials: L
authors.lastname: Li
authors.forename: Yaping
authors.initials: Y
authors.lastname: Xin
mesh_terms: D000483:Alleles; D000818:Animals; D001947:Breeding; D002417:Cattle / Q000235:genetics*; D002681:China; D005091:Exons; D005787:Gene Frequency; D005838:Genotype; D008460:Meat / Q000032:analysis*; D010641:Phenotype; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D011333:Pro-Opiomelanocortin / Q000235:genetics*; D017422:Sequence Analysis, DNA; D014465:Ultrasonics
chemical_list: D011333:Pro-Opiomelanocortin
keywords: ACD; Cattle; DNA marker; GLM; HWE; Hardy–Weinberg equilibriums; JR; Jiaxian red cattle; LD; LX; Luxi cattle, SL, Simmental and Luxi crossbred steers; Meat quality; NY; Nanyang cattle; PCR; PIC; POMC; POMC gene; QC; QI; Qinchuan cattle; Qinchuan improvement steers; SNP; Single nucleotide polymorphism; UBF; ULMA; UMAR; XN; Xia'nan cattle; acid citrate dextrose; general linear model; linkage disequilibrium; polymerase chain reaction; polymorphism information content; proopiomelanocortin; ultrasound backfat thickness; ultrasound loin muscle area; ultrasound marbling score