title: Effects of Salvia officinalis and Thymus vulgaris on oxidant-induced DNA damage and antioxidant status in HepG2 cells.
abstract: Salvia officinalis (SO) and Thymus vulgaris (TV) are medicinal plants well known for their curative powers. However, the molecular mechanisms responsible for these abilities of sage and thyme have not been fully understood yet. In this study we investigated the composition and the quantitative estimation of plant extracts, the protective effects of plant extracts against hydrogen peroxide- and 2,3-dimethoxy-1,4-naphthoquinone-induced DNA damage, and levels of enzymatic and non-enzymatic antioxidants (superoxide dismutase, glutathione peroxidase, glutathione) in human HepG2 cells. To measure antioxidative activity of plant extracts we used three assays: 1,1-diphenyl-2-picrylhydrazyl (DPPH), ferric reducing antioxidant power (FRAP) and 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS). The results showed that the oxidant-induced DNA lesions were significantly reduced in cells pre-treated with the plant extracts studied. The observed DNA-protective activity could be explained by both elevation of GPx activity in cells pre-treated with SO and TV and antioxidant activity of SO and TV.
authors.affiliation: Cancer Research Institute, Slovak Academy of Sciences, Vlárska 7, 833 91 Bratislava, Slovak Republic. katarina.kozics@savba.sk
authors.forename: Katarína
authors.initials: K
authors.lastname: Kozics
authors.forename: Veronika
authors.initials: V
authors.lastname: Klusová
authors.forename: Annamária
authors.initials: A
authors.lastname: Srančíková
authors.forename: Pavol
authors.initials: P
authors.lastname: Mučaji
authors.forename: Darina
authors.initials: D
authors.lastname: Slameňová
authors.forename: Lubica
authors.initials: L
authors.lastname: Hunáková
authors.forename: Barbara
authors.initials: B
authors.lastname: Kusznierewicz
authors.forename: Eva
authors.initials: E
authors.lastname: Horváthová
mesh_terms: D000975:Antioxidants / Q000378:metabolism*; D004249:DNA Damage / Q000187:drug effects*; D005978:Glutathione / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D056945:Hep G2 Cells; D006801:Humans; D006861:Hydrogen Peroxide / Q000633:toxicity; D009285:Naphthoquinones / Q000633:toxicity; D016877:Oxidants / Q000633:toxicity*; D010936:Plant Extracts / Q000494:pharmacology*; D027543:Salvia officinalis / Q000737:chemistry*; D013482:Superoxide Dismutase / Q000378:metabolism; D046930:Thymus Plant / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D009285:Naphthoquinones; D016877:Oxidants; D010936:Plant Extracts; C063002:2,3-dimethoxy-1,4-naphthoquinone; D006861:Hydrogen Peroxide; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005978:Glutathione
keywords: Antioxidant activity; Antioxidant enzymes; Comet assay; Human hepatoma HepG2 cells; Salvia officinalis extract; Thymus vulgaris extract