title: SUMOylation of damaged DNA-binding protein DDB2.
abstract: Damaged DNA-binding protein (DDB) is a heterodimer composed of two subunits, p127 and p48, which have been designated DDB1 and DDB2, respectively. DDB2 recognizes and binds to UV-damaged DNA during nucleotide excision repair. Here, we demonstrated that DDB2 was SUMOylated in a UV-dependent manner, and its major SUMO E3 ligase was PIASy as determined by RNA interference-mediated knockdown. The UV-induced physical interaction between DDB2 and PIASy supported this notion. PIASy knockdown reduced the removal of cyclobutane pyrimidine dimers (CPDs) from total genomic DNA, but did not affect that of 6-4 pyrimidine pyrimidone photoproducts (6-4PPs). Thus, DDB2 plays an indispensable role in CPD repair, but not in 6-4PP repair, which is consistent with the observation that DDB2 was SUMOylated by PIASy. These results suggest that the SUMOylation of DDB2 facilitates CPD repair.
authors.affiliation: Department of Biotechnology, Graduate School of Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8603, Japan.
authors.forename: Maasa
authors.initials: M
authors.lastname: Tsuge
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Masuda
authors.forename: Hidenori
authors.initials: H
authors.lastname: Kaneoka
authors.forename: Shunsuke
authors.initials: S
authors.lastname: Kidani
authors.forename: Katsuhide
authors.initials: K
authors.lastname: Miyake
authors.forename: Shinji
authors.initials: S
authors.lastname: Iijima
mesh_terms: D004247:DNA / Q000502:physiology* / Q000528:radiation effects*; D004249:DNA Damage / Q000502:physiology*; D004260:DNA Repair / Q000502:physiology* / Q000528:radiation effects; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D011829:Radiation Dosage; D025842:SUMO-1 Protein / Q000235:genetics / Q000378:metabolism*; D058207:Sumoylation / Q000502:physiology* / Q000528:radiation effects; D014466:Ultraviolet Rays
chemical_list: C117669:DDB2 protein, human; D004268:DNA-Binding Proteins; D025842:SUMO-1 Protein; D004247:DNA
keywords: DDB2; NER; PIASy; SUMOylation; UV damage