title: Differential diagnosis of reactive mesothelial cells and malignant mesothelioma cells using the cell proliferation markers minichromosome maintenance protein 7, geminin, topoisomerase II alpha and Ki-67.
abstract: OBJECTIVE
  The aim of this study was to evaluate whether the immunocytochemical expression of cell proliferation markers, such as minichromosome maintenance protein 7 (MCM 7), geminin, topoisomerase II alpha (topo IIα) and Ki-67, which are different types of cell proliferation markers, could be useful for their differential diagnosis in reactive mesothelial cells and malignant mesothelioma cells obtained from body cavity fluids.
  
  
  METHODS
  Samples diagnosed and later histologically confirmed as reactive mesothelial cells (39 cases) or malignant mesothelioma (32 cases) in body cavity fluids were examined. Immunocytochemical staining of MCM 7, geminin, topo IIα and Ki-67 was performed with the immunoperoxidase polymer method.
  
  
  RESULTS
  Labeling indices (LIs) of MCM 7 (cutoff value 20.0%; sensitivity 100%; specificity 100%), geminin (cutoff value 4.5%; sensitivity 88.0%; specificity 70.0%), topo IIα (cutoff value 11.0%; sensitivity 88.0%; specificity 92.0%) and Ki-67 (cutoff value 15.3%; sensitivity 78.0%; specificity 79.0%) of malignant mesothelioma cells were significantly higher than those of reactive mesothelial cells.
  
  
  CONCLUSIONS
  LIs of MCM 7, geminin and topo IIα can be reliable tools for the differential diagnosis of reactive mesothelial cells and malignant mesothelioma cells.
authors.affiliation: Department of Pathological Analysis, Division of Medical Life Sciences, Hirosaki University Graduate School of Health Sciences, Hirosaki, Japan. kimura.f.aa@m.titech.ac.jp
authors.forename: Fumikazu
authors.initials: F
authors.lastname: Kimura
authors.forename: Isao
authors.initials: I
authors.lastname: Okayasu
authors.forename: Hirokuni
authors.initials: H
authors.lastname: Kakinuma
authors.forename: Yukitoshi
authors.initials: Y
authors.lastname: Satoh
authors.forename: Sadahito
authors.initials: S
authors.lastname: Kuwao
authors.forename: Makoto
authors.initials: M
authors.lastname: Saegusa
authors.forename: Jun
authors.initials: J
authors.lastname: Watanabe
mesh_terms: D000368:Aged; D000951:Antigens, Neoplasm / Q000032:analysis / Q000096:biosynthesis; D001202:Ascitic Fluid / Q000378:metabolism / Q000473:pathology; D014408:Biomarkers, Tumor / Q000032:analysis*; D018797:Cell Cycle Proteins / Q000032:analysis / Q000096:biosynthesis; D049109:Cell Proliferation; D004250:DNA Topoisomerases, Type II / Q000032:analysis / Q000096:biosynthesis; D004268:DNA-Binding Proteins / Q000032:analysis / Q000096:biosynthesis; D003937:Diagnosis, Differential; D004848:Epithelium / Q000378:metabolism / Q000473:pathology*; D005260:Female; D064248:Geminin; D006801:Humans; D007090:Image Interpretation, Computer-Assisted; D007150:Immunohistochemistry; D019394:Ki-67 Antigen / Q000032:analysis / Q000096:biosynthesis; D008297:Male; D008654:Mesothelioma / Q000175:diagnosis* / Q000378:metabolism; D008875:Middle Aged; D064168:Minichromosome Maintenance Complex Component 7; D009687:Nuclear Proteins / Q000032:analysis / Q000096:biosynthesis; D010996:Pleural Effusion / Q000378:metabolism / Q000473:pathology; D012680:Sensitivity and Specificity
chemical_list: D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; D018797:Cell Cycle Proteins; D004268:DNA-Binding Proteins; C577995:GMNN protein, human; D064248:Geminin; D019394:Ki-67 Antigen; D009687:Nuclear Proteins; C101624:MCM7 protein, human; D064168:Minichromosome Maintenance Complex Component 7; D004250:DNA Topoisomerases, Type II