title: The evaluation of the FOXO1, KLF9 and YT521 genes expression in human endometrial cancer.
abstract: BACKGROUND
  The current study was designed to explore the changes of the mRNA levels of the YT521, Forkhead box protein O1 (FOXO1), and Krüppel-like factor 9 (KLF9) proteins in human normal and cancerous endometrial tissue.
  
  
  METHODS
  The study was conducted in 30 premenopausal patients diagnosed with endometrial cancer and 20 premenopausal women with no clinically documented abnormalities of the endometrium undergoing hysterectomy. Gene expression levels were assayed using quantitative real-time PCR.
  
  
  RESULTS
  The endometrial tissue FOXO1 mRNA level (0.82 +/- 0.27) of patients with endometrial cancer was significantly lower (p < 0.001) than controls (4.51 +/- 2.68). In subjects with endometrial cancer the KLF9 mRNA level (1.12 +/- 0.38) was lower (p < 0.001) when compared to controls (3.11 +/- 1.52). A remarkable (not significant, p = 0.069) increase was found in the YT521 mRNA level of patients' endometrial tissue (11.19 +/- 3.99) in comparison with the control subjects (8.82 +/- 5.01). No significant difference was detected for the FOXO1, KLF9 and YT521 mRNA levels of the endometrial tissue of patients with cancer at different stages.
  
  
  CONCLUSIONS
  It is suggested that the alteration of the gene expression profiles of FOXO1, KLF9 and YT521, which occur in human endometrial cancers likely play a crucial role in initiation of cancer.
authors.affiliation: Biochemistry Department of Medicine Faculty, Tehran University of Medical Sciences, Hemmat Highway, Tehran, Iran.
authors.forename: Mohsen
authors.initials: M
authors.lastname: Korani
authors.forename: Soudabeh
authors.initials: S
authors.lastname: Fallah
authors.forename: Afsaneh
authors.initials: A
authors.lastname: Tehranian
authors.forename: Mitra
authors.initials: M
authors.lastname: Nourbakhsh
authors.forename: Ali
authors.initials: A
authors.lastname: Samadikuchaksaraei
authors.forename: Maryam Sanjary
authors.initials: MS
authors.lastname: Pour
authors.forename: Jafar
authors.initials: J
authors.lastname: Maleki
mesh_terms: D000704:Analysis of Variance; D016022:Case-Control Studies; D016889:Endometrial Neoplasms / Q000235:genetics* / Q000378:metabolism; D005260:Female; D000071161:Forkhead Box Protein O1; D051858:Forkhead Transcription Factors / Q000096:biosynthesis / Q000235:genetics*; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D051741:Kruppel-Like Transcription Factors / Q000096:biosynthesis / Q000235:genetics*; D009419:Nerve Tissue Proteins / Q000096:biosynthesis / Q000235:genetics*; D000072260:RNA Splicing Factors; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics; D016601:RNA-Binding Proteins / Q000096:biosynthesis / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction
chemical_list: C085126:FOXO1 protein, human; D000071161:Forkhead Box Protein O1; D051858:Forkhead Transcription Factors; C489676:KLF9 protein, human; D051741:Kruppel-Like Transcription Factors; D009419:Nerve Tissue Proteins; D000072260:RNA Splicing Factors; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; C490769:YTHDC1 protein, human