title: Water-soluble and biocompatible MnO@PVP nanoparticles for MR imaging in vitro and in vivo.
abstract: The uniform-sized manganese oxide nanoparticles (the oleic-capped MnO NPs) were synthesized by the thermal decomposition of Mn-oleate complex and were transferred into water with the help of cationic surfactant of cetyltrimethyl ammonium bromide (CTAB), then the poly(vinylpyrrolidone) (PVP) membrane was further coated on to them with the aid of anionic dispersant of poly(styrenesulfonate) (PSS) by layer-by-layer electrostatic assembly to render them water soluble and biocompatible. They were characterized by transmission electron microscopy (TEM), dynamic light scattering (DLS), X-ray diffraction (XRD), Fourier-transform infrared (FTIR) and MTT assay. In vitro cellular uptake test revealed the MnO@PVP NPs were low cytotoxic, biocompatible and could be used as a T,-positive contrast agent for passive targeting magnetic resonance imaging (MRI). Interestingly, signal enhancement in cerebral spinal fluid (CSF) spaces in vivo experiment suggested that the MnO@PVP NPs can pass through the blood brain barrier (BBB). These results show that MnO@PVP NPs are good candidates as MRI contrast agents with the lack of cytotoxicity and have great potential applications in magnetic nano-device and biomagnetic field.
authors.affiliation: Education Ministry Key Laboratory of Resource Chemistry, Department of Chemistry, Life and Environmental Science College, Shanghai Normal University, Shanghai 200234, China.
authors.forename: Xiaoqing
authors.initials: X
authors.lastname: Hu
authors.forename: Yuxuan
authors.initials: Y
authors.lastname: Ji
authors.forename: Mingliang
authors.initials: M
authors.lastname: Wang
authors.forename: Fei
authors.initials: F
authors.lastname: Miao
authors.forename: Hongmei
authors.initials: H
authors.lastname: Ma
authors.forename: Hebai
authors.initials: H
authors.lastname: Shen
authors.forename: Nengqin
authors.initials: N
authors.lastname: Jia
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000138:chemical synthesis; D003287:Contrast Media / Q000138:chemical synthesis*; D008279:Magnetic Resonance Imaging / Q000379:methods*; D017895:Manganese Compounds / Q000737:chemistry*; D051379:Mice; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010087:Oxides / Q000737:chemistry*; D010316:Particle Size; D011205:Povidone / Q000737:chemistry*; D012995:Solubility; D014867:Water / Q000737:chemistry; D051598:Whole Body Imaging / Q000379:methods
chemical_list: D001672:Biocompatible Materials; D003287:Contrast Media; D017895:Manganese Compounds; D010087:Oxides; D014867:Water; C027424:manganese oxide; D011205:Povidone