title: Combined use of cyclophosphamide and Chalone 19-peptide in experimental breast cancer.
abstract: BACKGROUND
  Cyclophosphamide is a potent anticancer drug, but its clinical utility is limited because of its severe side effects, in particular liver damage. Chalone 19-peptide induces apoptosis of tumor cells and inhibits tumor growth. The present study investigated the antitumor effects of a combination of cyclophosphamide and Chalone 19-peptide in experimental breast cancer.
  
  
  METHODS
  An animal model of breast cancer was developed, consisting of an MDA-MB-231 cell line implanted in the nude mouse. Eight doses of a combination of cyclophosphamide 50 mg/kg or 100 mg/kg and Chalone 19-peptide 6.6 mg/kg were administered, and the mice were euthanized 28 days after the final drug injection. Histopathologic analysis of tumor size, metastasis, and apoptosis of cancer cells was performed. Control mice were injected intraperitoneally with either cyclophosphamide alone or the same volume of solvent.
  
  
  RESULTS
  Tumor sizes in the treatment groups were smaller than in the controls. No metastasis was found in the groups treated with cyclophosphamide and Chalone 19-peptide, but lung metastasis was found in controls. Liver damage in the groups treated with cyclophosphamide was more serious than in the other groups.
  
  
  CONCLUSIONS
  Addition of Chalone 19-peptide can improve the ability of cyclophosphamide to inhibit tumor growth and also reduces side effects.
authors.affiliation: Third Affiliated Hospital.
authors.forename: Yuanxi
authors.initials: Y
authors.lastname: Huang
authors.forename: Yan
authors.initials: Y
authors.lastname: He
authors.forename: Shengqian
authors.initials: S
authors.lastname: Ye
authors.forename: Xiaomei
authors.initials: X
authors.lastname: Li
authors.forename: Qingqi
authors.initials: Q
authors.lastname: Zhong
authors.forename: Zhuo
authors.initials: Z
authors.lastname: Chen
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Jin
keywords: Chalone 19-peptide; apoptosis; cyclophosphamide; hepatic injury; tumor suppression