title: Effects of chitosan coatings on polypropylene mesh for implantation in a rat abdominal wall model.
abstract: Hernia repair and pelvic floor reconstruction are usually accompanied with the implantation of a surgical mesh, which frequently results in a foreign body response with associated complications. An ideal surgical mesh that allows force generation of muscle tissues without significant granulation tissue and/or fibrosis is of significant clinical interest. The objective of the present study was to evaluate the in vitro and in vivo responses of a chitosan coating on polypropylene mesh (Ch-PPM) in comparison with commercially available meshes. We found that application of a 0.5% (w/v) Ch-PPM elicited preferential attachment of myoblasts over fibroblast attachment in vitro. Therefore, we test the hypothesis that 0.5% Ch-PPM will encourage skeletal muscle tissue ingrowth and decrease fibrosis formation in vivo. We implanted 0.5% Ch-PPM, collagen-coated polypropylene mesh (Pelvitex™; C.R. Bard), and polypropylene (Avaulta Solo(®); C.R. Bard) alone using a rat abdominal defect model. Force generation capacity and inflammatory response of each mesh were evaluated 2, 4, and 12 weeks postimplantation. We found that chitosan coating is associated with the restoration of functional skeletal muscle with histomorphologic characteristics that resemble native muscle and an early macrophage phenotypic response that has previously been shown to lead to more functional outcomes.
authors.affiliation: 1 Sibley School of Mechanical and Aerospace Engineering, Cornell University , Ithaca, New York.
authors.forename: Natasha
authors.initials: N
authors.lastname: Udpa
authors.forename: Shama R
authors.initials: SR
authors.lastname: Iyer
authors.forename: Rohit
authors.initials: R
authors.lastname: Rajoria
authors.forename: Kate E
authors.initials: KE
authors.lastname: Breyer
authors.forename: Helen
authors.initials: H
authors.lastname: Valentine
authors.forename: Bhupinder
authors.initials: B
authors.lastname: Singh
authors.forename: Sean P
authors.initials: SP
authors.lastname: McDonough
authors.forename: Bryan N
authors.initials: BN
authors.lastname: Brown
authors.forename: Lawrence J
authors.initials: LJ
authors.lastname: Bonassar
authors.forename: Yingxin
authors.initials: Y
authors.lastname: Gao
mesh_terms: D034861:Abdominal Wall / Q000473:pathology / Q000601:surgery*; D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D048271:Chitosan / Q000737:chemistry / Q000494:pharmacology*; D020099:Coated Materials, Biocompatible / Q000737:chemistry / Q000494:pharmacology*; D005347:Fibroblasts / Q000378:metabolism / Q000473:pathology; D007249:Inflammation / Q000378:metabolism / Q000473:pathology; D008422:Materials Testing; D051379:Mice; D032448:Myoblasts, Skeletal / Q000378:metabolism / Q000473:pathology; D011126:Polypropylenes / Q000737:chemistry / Q000494:pharmacology*; D051381:Rats; D013526:Surgical Mesh
chemical_list: D020099:Coated Materials, Biocompatible; D011126:Polypropylenes; D048271:Chitosan