title: Single cell gene profiling revealed heterogeneity of paracrine effects of bone marrow cells in mouse infarcted hearts.
abstract: It is now recognized that transplantation of bone marrow cells (BMCs) into infarcted hearts has the capacity to improve the cardiac function through paracrine effects. However, detailed expression levels of paracrine factors in BMCs in infarcted hearts are poorly described. By use of laser capture microdissection combined with real-time PCR, we depicted the expression profiles of paracrine factors in infarcted hearts versus normal hearts. Consistent with the in vivo observation, a similar expression pattern was evidenced in cultured BMCs. Furthermore, BMCs displayed heterogeneity of paracrine effects in infarcted hearts as analyzed at the single cell level using single cell PCR. Interestingly, the CD45(+) subpopulation showed higher expression levels of angiogenic factors compared to other subpopulations. Finally, most angiogenic factors were induced under the microenvironment of infarction. Our study demonstrated the heterogeneity of paracrine effects in BMCs at single cell level in infarcted hearts, highlighting preferential expression of angiogenic factors in the CD45(+) subpopulation. These findings broaden our understanding of paracrine effects of BMCs in vivo, and offer new insights into BMCs therapy in myocardial infarction (MI).
authors.affiliation: Institute of Geriatric Cardiology, the General Hospital of Chinese People's Liberation Army (PLA), Beijing, China.
authors.forename: Yanhua
authors.initials: Y
authors.lastname: Li
authors.forename: Xinhong
authors.initials: X
authors.lastname: Guo
authors.forename: Qiao
authors.initials: Q
authors.lastname: Xue
authors.forename: Mei
authors.initials: M
authors.lastname: Zhu
authors.forename: Lei
authors.initials: L
authors.lastname: Gao
authors.forename: Yu
authors.initials: Y
authors.lastname: Wang
mesh_terms: D043925:Angiogenesis Inducing Agents / Q000378:metabolism; D000818:Animals; D001854:Bone Marrow Cells / Q000378:metabolism*; D016026:Bone Marrow Transplantation; D016000:Cluster Analysis; D004195:Disease Models, Animal; D005260:Female; D006321:Heart / Q000503:physiopathology; D000860:Hypoxia; D017493:Leukocyte Common Antigens / Q000378:metabolism; D051379:Mice; D009203:Myocardial Infarction / Q000235:genetics* / Q000378:metabolism*; D009206:Myocardium / Q000378:metabolism / Q000473:pathology; D009389:Neovascularization, Pathologic; D019899:Paracrine Communication; D059010:Single-Cell Analysis; D059467:Transcriptome
chemical_list: D043925:Angiogenesis Inducing Agents; D017493:Leukocyte Common Antigens