title: PAP/HIP protein is an obesogenic factor.
abstract: In this article we report the obesogenic role of the acute phase protein PAP/HIP. We found that the transgenic TgPAP/HIP mice develop spontaneous obesity under standard nutritional conditions, with high levels of glucose, leptin, and LDL and low levels of triglycerides and HDL in blood. Accordingly, PAP/HIP-deficient mice are skinny under standard nutritional conditions. We also found that expression of PAP/HIP is induced in intestinal epithelial cells in response to gavage with olive oil and this induction is AG490 sensitive. We demonstrated that incubation of 3T3-L1 preadipocytes with a low concentration as 1 ng/ml of recombinant PAP/HIP results in accelerated BrdU incorporation in vitro. PAP/HIP-dependent adipocytes growth is sensitive to the MEK inhibitor U0126. Finally, patients with severe obesity present higher blood levels of PAP/HIP than non-obese control individuals. Altogether our data suggest that PAP/HIP could be a mediator of fat tissue development, released by the intestine and induced by the presence of food into the gut.
authors.affiliation: Centre de Recherche en Carcérologie de Marseille (CRCM), INSERM UMR 1068, CNRS UMR 7258, Aix-Marseille University and Institut Paoli-Calmettes, Parc Scientifique et Technologique de Luminy, Marseille, France.
authors.forename: Veronique
authors.initials: V
authors.lastname: Secq
authors.forename: Cecilia
authors.initials: C
authors.lastname: Mallmann
authors.forename: Meritxell
authors.initials: M
authors.lastname: Gironella
authors.forename: Belen
authors.initials: B
authors.lastname: Lopez
authors.forename: Daniel
authors.initials: D
authors.lastname: Closa
authors.forename: Stéphane
authors.initials: S
authors.lastname: Garcia
authors.forename: Laurence
authors.initials: L
authors.lastname: Christa
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Montalto
authors.forename: Nelson
authors.initials: N
authors.lastname: Dusetti
authors.forename: Juan L
authors.initials: JL
authors.lastname: Iovanna
mesh_terms: D041721:3T3-L1 Cells; D000273:Adipose Tissue / Q000378:metabolism; D000328:Adult; D000818:Animals; D001779:Blood Coagulation Factors / Q000235:genetics / Q000378:metabolism*; D002070:Butadienes; D005260:Female; D005786:Gene Expression Regulation / Q000502:physiology; D006801:Humans; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D008822:Mice, Transgenic; D008875:Middle Aged; D009570:Nitriles; D009765:Obesity / Q000235:genetics / Q000378:metabolism*; D000073718:Pancreatitis-Associated Proteins; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D016601:RNA-Binding Proteins; D012269:Ribosomal Proteins
chemical_list: D001779:Blood Coagulation Factors; D002070:Butadienes; D009570:Nitriles; D000073718:Pancreatitis-Associated Proteins; D011506:Proteins; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; C104946:RPL29 protein, human; C490253:Reg3b protein, mouse; D012269:Ribosomal Proteins; C113580:U 0126