title: Assessment of DNA damage and lipid peroxidation in diabetic mice: effects of propolis and epigallocatechin gallate (EGCG).
abstract: There is growing recognition that polyphenolic compounds present in many plants and natural products may have beneficial effects on human health. Propolis - a substance produced by honeybees - and catechins in tea, in particular (-)-epigallocatechin gallate (EGCG), are strong antioxidants that appear to have anti-obesity and anti-diabetic effects. The present study was designed to elucidate the anti-diabetic effect of the water-soluble derivative of propolis (WSDP), which contains phenolic acids as the main compounds, and EGCG in alloxan-induced (75mg/kg, iv) diabetes in mice. Intraperitoneal administration of EGCG or propolis at doses of 50mg/kg body weight (bw) to diabetic mice for a period of 7 days resulted in a significant increase in body weight and in haematological/immunological blood parameters, as well as in 100% survival of the mice. A significant decrease in lipid peroxidation in liver, kidney and brain tissue was also observed in diabetic mice treated with these two agents. Additionally, EGCG and propolis clearly reduced DNA damage in peripheral lymphocytes of diabetic mice. Our studies demonstrate the anti-oxidative and anti-inflammatory potential of WSDP and EGCG, which could exert beneficial effects against diabetes and the associated consequences of free-radical formation in kidney, liver, spleen and brain tissue. The results suggest that dietary supplementation with WSDP or EGCG could potentially contribute to nutritional strategies for the prevention and treatment of diabetes mellitus.
authors.affiliation: Department of Animal Physiology, Faculty of Science, University of Zagreb, Rooseveltov trg 6, 10000 Zagreb, Croatia. Electronic address: norsolic@yahoo.com.
authors.forename: Nada
authors.initials: N
authors.lastname: Oršolić
authors.forename: Damir
authors.initials: D
authors.lastname: Sirovina
authors.forename: Goran
authors.initials: G
authors.lastname: Gajski
authors.forename: Vera
authors.initials: V
authors.lastname: Garaj-Vrhovac
authors.forename: Maja
authors.initials: M
authors.lastname: Jazvinšćak Jembrek
authors.forename: Ivan
authors.initials: I
authors.lastname: Kosalec
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage*; D001516:Bees; D001921:Brain / Q000187:drug effects; D002392:Catechin / Q000008:administration & dosage / Q000031:analogs & derivatives*; D004249:DNA Damage / Q000187:drug effects; D006801:Humans; D007668:Kidney / Q000187:drug effects; D015227:Lipid Peroxidation / Q000187:drug effects*; D008099:Liver / Q000187:drug effects; D051379:Mice; D016688:Mice, Inbred NOD; D011429:Propolis / Q000008:administration & dosage*
chemical_list: D000975:Antioxidants; D002392:Catechin; D011429:Propolis; C045651:epigallocatechin gallate
keywords: AO; Alloxan; DNA damage; Diabetes; EGCG; EtBr; HPLC; IDDM; LMP; LPO; Lipid peroxidation; MCH; MCHC; MCV; MDA; MN; NMP; Propolis; ROS; TBA; TC; TG; WSDP; acridine orange; epigallocatechin gallate; ethidium bromide; high-performance liquid chromatography; insuline-dependent diabetes mellitus; lipid peroxidation; low melting-point; malondialdehyde; mean corpuscular hemoglobin; mean corpuscular hemoglobin concentration; mean corpuscular volume; micronucleus; normal melting-point; reactive oxygen species; thiobarbituric acid; total cholesterol; triglyceride; water-soluble derivative of propolis