title: Association of lysyl oxidase-like 1 gene common sequence variants in Greek patients with pseudoexfoliation syndrome and pseudoexfoliation glaucoma.
abstract: OBJECTIVE
  Three common sequence variants in the lysyl oxidase-like 1 (LOXL1) gene were recently associated with pseudoexfoliation (PEX) and pseudoexfoliation glaucoma (PEXG) in populations from various parts of the world. In this study, the genetic association of these variants was investigated in Greek patients with PEX and PEXG.
  
  
  METHODS
  The three LOXL1 single nucleotide polymorphisms (SNPs), one intronic (rs2165241) and two nonsynonymous coding SNPs (rs1048661: R141L and rs3825942: G153D), were genotyped in a total of 48 unrelated patients with PEX, 35 patients with PEXG, and 52 healthy subjects who had normal findings in repeated ophthalmic examinations. A genetic association study was performed.
  
  
  RESULTS
  Between the two coding SNPs, R141L did not show an association with PEX (p=0.297 for allele G, p=0.339 for genotype GG), whereas allele G of G153D showed a significant association (odds ratio [OR]=3.52, 95% confidence interval [CI]=1.735-7.166, p=3.24×10(-4) for allele G, p=0.004 for genotype GG). Likewise, for the intronic SNP of rs2165241, genotype TT (p=0.005) and its corresponding allele T (OR=2.99, 95% CI=1.625-5.527, p=3.53×10(-4)) showed a significant association with PEX. The allele G of G153D showed a significant association with PEXG (OR=3.74, 95% CI=1.670-8.387, p=0.001). The combined haplotype GGT, consisting of all three risk alleles, was associated with PEX (p=0.037), conferring a 1.8-fold of increased risk to the disease (OR=1.799, 95% CI=1.04-3.13). Furthermore, the haplotype GGT presented in 39.8% of the patients with PEX and 26.9% of the controls.
  
  
  CONCLUSIONS
  Certain genetic variants in LOXL1 confer risk for PEX in Greek populations, confirming in part findings in patients from Northern Europe.
authors.affiliation: Ophthalmiatrion Athinon, Athens Eye Hospital, Athens, Greece.
authors.forename: Ioanna
authors.initials: I
authors.lastname: Metaxaki
authors.forename: Pantelis
authors.initials: P
authors.lastname: Constantoulakis
authors.forename: Miltiadis
authors.initials: M
authors.lastname: Papadimitropoulos
authors.forename: Eftihia
authors.initials: E
authors.lastname: Filiou
authors.forename: Gerasimos
authors.initials: G
authors.lastname: Georgopoulos
authors.forename: Aggeliki
authors.initials: A
authors.lastname: Chamchougia
authors.forename: Dimitrios
authors.initials: D
authors.lastname: Papakonstantinou
authors.forename: Nikos
authors.initials: N
authors.lastname: Markomichelakis
authors.forename: Chrysanthi
authors.initials: C
authors.lastname: Koutsandrea
authors.forename: Ioannis
authors.initials: I
authors.lastname: Halkiadakis
mesh_terms: D000368:Aged; D000594:Amino Acid Oxidoreductases / Q000235:genetics*; D001483:Base Sequence; D016022:Case-Control Studies; D003710:Demography; D017889:Exfoliation Syndrome / Q000150:complications / Q000201:enzymology / Q000235:genetics*; D005260:Female; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D005901:Glaucoma / Q000150:complications / Q000201:enzymology / Q000235:genetics*; D006115:Greece; D006239:Haplotypes / Q000235:genetics; D006801:Humans; D008297:Male; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D012307:Risk Factors
chemical_list: D000594:Amino Acid Oxidoreductases; C445313:LOXL1 protein, human