title: Ethionine regulates cell motile activity through LPA receptor-3 in liver epithelial WB-F344 cells.
abstract: Lysophosphatidic acid (LPA) receptors (LPA1 to LPA6) indicate a variety of cellular responses, such as cell proliferation, migration, differentiation, and morphogenesis. However, the role of each LPA receptor is not functionally equivalent. Ethionine, an ethyl analog of methionine, is well known to be one of the potent liver carcinogens in rats. In this study, to assess whether ethionine may regulate cell motile activity through LPA receptors, rat liver epithelial (WB-F344) cells were treated with ethionine for 48 h. In cell motility assay with a cell culture insert, the treatment of ethionine at 1.0 and 10 μM enhanced significantly high cell motile activity, compared with untreated cells. The expression levels of LPA receptor genes in cells treated with ethionine were measured by quantitative real time RT-PCR analysis. The expression of the Lpar3 gene in ethionine-treated cells was significantly higher than that in untreated cells. Furthermore, to confirm an involvement of LPA3 on cell motility increased by ethionine, the Lpar3 knockdown cells were also used. The cell motile activity by ethionine was completely suppressed in the Lpar3 knockdown cells. These results suggest that LPA signaling through LPA3 may be involved in cell motile activity stimulated by ethionine in WB-F344 cells.
authors.affiliation: Division of Cancer Biology and Bioinformatics, Department of Life Science, Faculty of Science and Engineering, Kinki University, 3-4-1, Kowakae, Higashiōsaka, Osaka, 577-8502, Japan.
authors.forename: Serina
authors.initials: S
authors.lastname: Inoue
authors.forename: Eriko
authors.initials: E
authors.lastname: Tanabe
authors.forename: Ayano
authors.initials: A
authors.lastname: Shibata
authors.forename: Miku
authors.initials: M
authors.lastname: Hirane
authors.forename: Mutsumi
authors.initials: M
authors.lastname: Araki
authors.forename: Yan
authors.initials: Y
authors.lastname: Dong
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Fukushima
authors.forename: Toshifumi
authors.initials: T
authors.lastname: Tsujiuchi
mesh_terms: D000818:Animals; D002460:Cell Line; D002465:Cell Movement / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D004847:Epithelial Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D005001:Ethionine / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects; D055785:Gene Knockdown Techniques; D008099:Liver / Q000166:cytology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D011916:Rats, Inbred F344; D049368:Receptors, Lysophosphatidic Acid / Q000235:genetics / Q000378:metabolism*
chemical_list: D012333:RNA, Messenger; D049368:Receptors, Lysophosphatidic Acid; D005001:Ethionine