title: Calcium phosphate/octadecyl-quatemized carboxymethyl chitosan nanoparticles: an efficient and promising carrier for gene transfection.
abstract: Calcium phosphate (CaP) has been widely used as the vector for gene transfection in the past three decades. However, clinical application is still not popular due to the poor-controlling of DNA/CaP complexes preparation, cytotoxicity and its low transfection efficiency. In this study, a novel amphipathic octadecyl-quatemized carboxymethyl chitosan (OQCMC) derivative from chitosan was combined with calcium phosphate to synthesize CaP/OQCMC nanoparticles (CaP/OQCMC NPs). The nanoparticles were 122-177 nm in diameter exhibited neutral zeta potential (from -0.115 mV to 0.216 mV), and they were applied as DNA vectors for DNA loading and in vitro transfection. The results showed that CaP/OQCMC NPs displayed high DNA loading capacity and enhanced transfection efficiency with extremely low cytotoxicity. In addition, both CaP and OQCMC are biocompatible and biodegradable, thus the as-prepared CaP/OQCMC NPs are promising in gene delivery.
authors.affiliation: Shanghai Cancer Institute, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200032, PR China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Sun
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Li
authors.forename: Xiaofei
authors.initials: X
authors.lastname: Liang
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Wan
authors.forename: Yourong
authors.initials: Y
authors.lastname: Duan
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D002130:Calcium Phosphates / Q000737:chemistry*; D002460:Cell Line; D048271:Chitosan / Q000031:analogs & derivatives* / Q000737:chemistry*; D004247:DNA / Q000737:chemistry; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D008027:Light; D051379:Mice; D046529:Microscopy, Electron, Transmission; D008957:Models, Genetic; D053761:Nanocomposites / Q000737:chemistry; D053758:Nanoparticles / Q000737:chemistry*; D036103:Nanotechnology / Q000379:methods*; D011108:Polymers / Q000737:chemistry; D012542:Scattering, Radiation; D014162:Transfection
chemical_list: D001672:Biocompatible Materials; D002130:Calcium Phosphates; D011108:Polymers; D004247:DNA; D048271:Chitosan; C020243:calcium phosphate