title: Characterization of the Burkholderia thailandensis SOS response by using whole-transcriptome shotgun sequencing.
abstract: The bacterial SOS response is a well-characterized regulatory network encoded by most prokaryotic bacterial species and is involved in DNA repair. In addition to nucleic acid repair, the SOS response is involved in pathogenicity, stress-induced mutagenesis, and the emergence and dissemination of antibiotic resistance. Using high-throughput sequencing technology (SOLiD RNA-Seq), we analyzed the Burkholderia thailandensis global SOS response to the fluoroquinolone antibiotic, ciprofloxacin (CIP), and the DNA-damaging chemical, mitomycin C (MMC). We demonstrate that a B. thailandensis recA mutant (RU0643) is ∼4-fold more sensitive to CIP in contrast to the parental strain B. thailandensis DW503. Our RNA-Seq results show that CIP and MMC treatment (P < 0.01) resulted in the differential expression of 344 genes in B. thailandensis and 210 genes in RU0643. Several genes associated with the SOS response were induced and include lexA, uvrA, dnaE, dinB, recX, and recA. At the genome-wide level, we found an overall decrease in gene expression, especially for genes involved in amino acid and carbohydrate transport and metabolism, following both CIP and MMC exposure. Interestingly, we observed the upregulation of several genes involved in bacterial motility and enhanced transcription of a B. thailandensis genomic island encoding a Siphoviridae bacteriophage designated E264. Using B. thailandensis plaque assays and PCR with B. mallei ATCC 23344 as the host, we demonstrate that CIP and MMC exposure in B. thailandensis DW503 induces the transcription and translation of viable bacteriophage in a RecA-dependent manner. This is the first report of the SOS response in Burkholderia spp. to DNA-damaging agents. We have identified both common and unique adaptive responses of B. thailandensis to chemical stress and DNA damage.
authors.affiliation: Integrated Toxicology Division, United States Army Medical Research Institute of Infectious Diseases (USAMRIID), Fort Detrick, Maryland, USA.
authors.forename: Ricky L
authors.initials: RL
authors.lastname: Ulrich
authors.forename: David
authors.initials: D
authors.lastname: Deshazer
authors.forename: Tara A
authors.initials: TA
authors.lastname: Kenny
authors.forename: Melanie P
authors.initials: MP
authors.lastname: Ulrich
authors.forename: Anna
authors.initials: A
authors.lastname: Moravusova
authors.forename: Timothy
authors.initials: T
authors.lastname: Opperman
authors.forename: Sina
authors.initials: S
authors.lastname: Bavari
authors.forename: Terry L
authors.initials: TL
authors.lastname: Bowlin
authors.forename: Donald T
authors.initials: DT
authors.lastname: Moir
authors.forename: Rekha G
authors.initials: RG
authors.lastname: Panchal
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001435:Bacteriophages / Q000235:genetics; D019117:Burkholderia / Q000187:drug effects / Q000235:genetics / Q000502:physiology*; D002939:Ciprofloxacin / Q000494:pharmacology; D016685:Mitomycin / Q000494:pharmacology; D009153:Mutagens; D014176:Protein Biosynthesis; D013014:SOS Response, Genetics; D017422:Sequence Analysis, DNA; D017906:Siphoviridae / Q000235:genetics; D014158:Transcription, Genetic; D059467:Transcriptome
chemical_list: D000900:Anti-Bacterial Agents; D009153:Mutagens; D016685:Mitomycin; D002939:Ciprofloxacin