title: Association between low SIRT1 expression in visceral and subcutaneous adipose tissues and metabolic abnormalities in women with obesity and type 2 diabetes.
abstract: OBJECTIVE
  To assess the importance of adipose tissue sirtuin 1 (SIRT1) in the regulation of whole-body metabolism in humans with obesity and type 2 diabetes.
  
  
  METHODS
  In total, 19 non-diabetic obese women, 19 type 2 diabetic women undergoing gastric bypass surgery, and 27 normal-weight women undergoing gynecological surgery (total 65 women) were enrolled. Their anthropometric variables, abdominal fat distribution and metabolic parameters, serum adiponectin concentrations, and SIRT1 mRNA and protein and adiponectin mRNA expressions in visceral adipose tissue (VAT) and subcutaneous adipose tissue (SAT) were measured.
  
  
  RESULTS
  SIRT1 mRNA levels in VAT and SAT were similar and these levels were suppressed in obese and type 2 diabetic women compared to normal-weight subjects. These decreases in SIRT1 expression were observed in both adipocytes and non-fat cells. There was a strong association between adipose tissue SIRT1 mRNA and protein levels. Adipose SIRT1 expression correlated inversely with HOMA-IR and other insulin resistance-related parameters. Adipose SIRT1 and adiponectin mRNA expression correlated very strongly and positively. SIRT1 mRNA level in VAT correlated inversely with visceral obesity whereas its expression in SAT correlated negatively with body mass index.
  
  
  CONCLUSIONS
  Adipose tissue SIRT1 may play a key role in the regulation of whole body metabolic homeostasis in humans. Downregulation of SIRT1 in VAT may contribute to the metabolic abnormalities that are associated with visceral obesity.
authors.affiliation: Department of Physiology, University of Ulsan College of Medicine, Seoul, South Korea.
authors.forename: Young Sook
authors.initials: YS
authors.lastname: Song
authors.forename: Seul Ki
authors.initials: SK
authors.lastname: Lee
authors.forename: Yeon Jin
authors.initials: YJ
authors.lastname: Jang
authors.forename: Hye Soon
authors.initials: HS
authors.lastname: Park
authors.forename: Jong-Hyeok
authors.initials: JH
authors.lastname: Kim
authors.forename: Yeon Ji
authors.initials: YJ
authors.lastname: Lee
authors.forename: Yoon-Suk
authors.initials: YS
authors.lastname: Heo
mesh_terms: D000328:Adult; D003924:Diabetes Mellitus, Type 2 / Q000378:metabolism*; D005260:Female; D006801:Humans; D007333:Insulin Resistance / Q000502:physiology; D050152:Intra-Abdominal Fat / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D009765:Obesity / Q000378:metabolism*; D056564:Sirtuin 1 / Q000378:metabolism*; D050151:Subcutaneous Fat / Q000378:metabolism*
chemical_list: D056564:Sirtuin 1
keywords: Adiponectin; Adipose tissue; Insulin resistance; SIRT1; Visceral obesity