title: Plasma lipid profiling in a large population-based cohort.
abstract: We have performed plasma lipid profiling using liquid chromatography electrospray ionization tandem mass spectrometry on a population cohort of more than 1,000 individuals. From 10 μl of plasma we were able to acquire comparative measures of 312 lipids across 23 lipid classes and subclasses including sphingolipids, phospholipids, glycerolipids, and cholesterol esters (CEs) in 20 min. Using linear and logistic regression, we identified statistically significant associations of lipid classes, subclasses, and individual lipid species with anthropometric and physiological measures. In addition to the expected associations of CEs and triacylglycerol with age, sex, and body mass index (BMI), ceramide was significantly higher in males and was independently associated with age and BMI. Associations were also observed for sphingomyelin with age but this lipid subclass was lower in males. Lysophospholipids were associated with age and higher in males, but showed a strong negative association with BMI. Many of these lipids have previously been associated with chronic diseases including cardiovascular disease and may mediate the interactions of age, sex, and obesity with disease risk.
authors.affiliation: Baker IDI Heart and Diabetes Institute, Melbourne, Australia.
authors.forename: Jacquelyn M
authors.initials: JM
authors.lastname: Weir
authors.forename: Gerard
authors.initials: G
authors.lastname: Wong
authors.forename: Christopher K
authors.initials: CK
authors.lastname: Barlow
authors.forename: Melissa A
authors.initials: MA
authors.lastname: Greeve
authors.forename: Adam
authors.initials: A
authors.lastname: Kowalczyk
authors.forename: Laura
authors.initials: L
authors.lastname: Almasy
authors.forename: Anthony G
authors.initials: AG
authors.lastname: Comuzzie
authors.forename: Michael C
authors.initials: MC
authors.lastname: Mahaney
authors.forename: Jeremy B M
authors.initials: JB
authors.lastname: Jowett
authors.forename: Jonathan
authors.initials: J
authors.lastname: Shaw
authors.forename: Joanne E
authors.initials: JE
authors.lastname: Curran
authors.forename: John
authors.initials: J
authors.lastname: Blangero
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Meikle
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001774:Blood Chemical Analysis; D015331:Cohort Studies; D005260:Female; D006801:Humans; D008055:Lipids / Q000097:blood*; D008297:Male; D017058:Mexican Americans; D008875:Middle Aged; D012015:Reference Standards; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization / Q000592:standards; D053719:Tandem Mass Spectrometry / Q000592:standards; D055815:Young Adult
chemical_list: D008055:Lipids
keywords: cardiovascular disease; disease risk; lipidomics; mass spectrometry; obesity