title: Inhaled lead exposure affects tracheal responsiveness and lung inflammation in guinea pigs during sensitization.
abstract: Total and differential white blood cells (WBC), and cytokines, levels in serum were examined in guinea pigs exposed to inhaled lead acetate. Different groups of guinea pigs including: control (group C), sensitized group (group S), and exposed animals to aerosol of three lead concentrations during sensitization (n = 6 for each group) were studied. Total and differential WBC counts of lung lavage, serum cytokine (IFNγ and IL-4), levels and tracheal responsiveness to methacholine and ovalbumin were measured. All measured values were significantly increased except for IFNγ/IL-4 ratio which was significantly decreased in nonexposed sensitized and those exposed to all lead concentrations compared to control group (p < 0.05 to p < 0.001). Most measured values in animals exposed to higher lead concentration were also significantly higher than group S except for tracheal responsiveness to methacholine and lymphocyte count. Lead concentration significantly increased in lung tissues of animals exposed to all three lead concentrations (p < 0.001 for all cases). These results showed that lead exposure during sensitization can induce greater increase in tracheal responsiveness, total WBC, eosinophil, neutrophil, and basophil counts as well as serum level of IL-4. It can also cause a decrease in lymphocyte count, IFNγ level, and IFNγ/IL-4 ratio especially in its high concentration. Therefore inhaled lead exposure may cause increased severity of asthma during development of the disease.
authors.affiliation: Applied Physiology Research Centre and Department of Physiology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran. boskabadymh@mums.ac.ir
authors.forename: Mohammad Hossein
authors.initials: MH
authors.lastname: Boskabaddy
authors.forename: Tahere
authors.initials: T
authors.lastname: Farkhondeh
mesh_terms: D000818:Animals; D004305:Dose-Response Relationship, Drug; D006168:Guinea Pigs; D019570:Inhalation Exposure / Q000009:adverse effects*; D007371:Interferon-gamma / Q000097:blood; D015847:Interleukin-4 / Q000097:blood; D007854:Lead / Q000008:administration & dosage / Q000378:metabolism / Q000633:toxicity*; D007958:Leukocyte Count; D007962:Leukocytes / Q000378:metabolism; D008168:Lung / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D016210:Methacholine Chloride / Q000008:administration & dosage / Q000633:toxicity; D009942:Organometallic Compounds / Q000008:administration & dosage / Q000633:toxicity; D010047:Ovalbumin / Q000008:administration & dosage / Q000633:toxicity; D011014:Pneumonia / Q000097:blood / Q000209:etiology*; D014133:Tracheal Diseases / Q000097:blood / Q000209:etiology*
chemical_list: D009942:Organometallic Compounds; D016210:Methacholine Chloride; D015847:Interleukin-4; D007854:Lead; D007371:Interferon-gamma; D010047:Ovalbumin; C008261:lead acetate