title: MAPLE-based method to obtain biodegradable hybrid polymeric thin films with embedded antitumoral agents.
abstract: In this work, antitumor compounds, lactoferrin [recombinant iron-free (Apo-rLf)], cisplatin (Cis) or their combination were embedded within a biodegradable polycaprolactone (PCL) polymer thin film, by a modified approach of a laser-based technique, matrix-assisted pulsed laser evaporation (MAPLE). The structural and morphological properties of the deposited hybrid films were analyzed by Fourier-transform infrared spectroscopy (FTIR) and atomic force microscopy (AFM). The in vitro effect on the cells' morphology and proliferation of murine melanoma B16-F10 cells was investigated and correlated with the films' surface chemistry and topography. Biological assays revealed decreased viability and proliferation, lower adherence, and morphological modifications in the case of melanoma cells cultured on both Apo-rLf and Cis thin films. The antitumor effect was enhanced by deposition of Apo-rLf with Cis within the same film. The unique capability of the new approach, based on MAPLE, to embed antitumor active factors within a biodegradable matrix for obtaining novel biodegradable hybrid platform with increased antitumor efficiency has been demonstrated.
authors.affiliation: INFLPR-National Institute for Lasers, Plasma and Radiation Physics, 409 Atomistilor st, Magurele, RO-077125, Bucharest, Romania, dincavalentina@yahoo.com.
authors.forename: Valentina
authors.initials: V
authors.lastname: Dinca
authors.forename: Paula E
authors.initials: PE
authors.lastname: Florian
authors.forename: Livia E
authors.initials: LE
authors.lastname: Sima
authors.forename: Laurentiu
authors.initials: L
authors.lastname: Rusen
authors.forename: Catalin
authors.initials: C
authors.lastname: Constantinescu
authors.forename: Robert W
authors.initials: RW
authors.lastname: Evans
authors.forename: Maria
authors.initials: M
authors.lastname: Dinescu
authors.forename: Anca
authors.initials: A
authors.lastname: Roseanu
mesh_terms: D000818:Animals; D016588:Anticarcinogenic Agents / Q000737:chemistry*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002945:Cisplatin / Q000737:chemistry; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D007781:Lactoferrin / Q000737:chemistry; D007834:Lasers; D051379:Mice; D018625:Microscopy, Atomic Force; D011091:Polyesters / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D013499:Surface Properties
chemical_list: D016588:Anticarcinogenic Agents; D020099:Coated Materials, Biocompatible; D011091:Polyesters; C016240:polycaprolactone; D007781:Lactoferrin; D002945:Cisplatin