title: [Study on the effect of Klotho gene interferred by plasmid-mediated short hairpin RNA (shRNA) on sinoatrial node pacing channel gene].
abstract: The study was aimed to assess the effect of Klotho gene and sinoatrial node pacing channel gene (HCN4 and HCN2) for studying sick sinus syndrome, with Klotho gene under the interference of Plasmid-mediated short hairpin RNA. Twenty-five C57BL/6J mice were divided into four groups, i. e, plasmid shRNA 24h group, plasmid shRNA 12h group, sodium chloride 24h group and sodium chloride 12h group. Plasmid shRNA 50microL (1microg/microL) and sodium chloride 50microl were respectively injected according to mice vena caudalis into those in plasmid shRNA group and sodium chloride group. After 12h or 24h respectively, all mice were executed and their sinoatrial node tissues were cut. The mRNA of Klotho, HCN4 and HCN2 gene were detected by RT-PCR. The results of RT-PCR showed that Klotho, HCN4 and HCN2 mRNA levels were lower compared with those in sodium chloride 12h group after 12h interference interval. The results indicated that there might be the a certain relationship between Klotho gene and sinoatrial node pacing channel gene.
authors.affiliation: Department of Geriatery, West China Hospital of Sichuan University, Chengdu 610041, China.
authors.forename: Yingying
authors.initials: Y
authors.lastname: Cai
authors.forename: Han
authors.initials: H
authors.lastname: Wang
authors.forename: Yanbin
authors.initials: Y
authors.lastname: Hou
authors.forename: Chenli
authors.initials: C
authors.lastname: Fang
authors.forename: Peng
authors.initials: P
authors.lastname: Tian
authors.forename: Guihua
authors.initials: G
authors.lastname: Wang
authors.forename: Lu
authors.initials: L
authors.lastname: Li
authors.forename: Juelin
authors.initials: J
authors.lastname: Deng
mesh_terms: D000818:Animals; D005966:Glucuronidase / Q000235:genetics*; D064428:Hyperpolarization-Activated Cyclic Nucleotide-Gated Channels / Q000235:genetics* / Q000378:metabolism; D000090265:Klotho Proteins; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D010957:Plasmids / Q000235:genetics*; D015221:Potassium Channels / Q000235:genetics* / Q000378:metabolism; D034622:RNA Interference; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D034741:RNA, Small Interfering / Q000235:genetics*; D012849:Sinoatrial Node / Q000378:metabolism / Q000502:physiology* / Q000503:physiopathology
chemical_list: C578711:Hcn2 protein, mouse; C578706:Hcn4 protein, mouse; D064428:Hyperpolarization-Activated Cyclic Nucleotide-Gated Channels; D015221:Potassium Channels; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D005966:Glucuronidase; D000090265:Klotho Proteins