title: An isotopically tagged azobenzene-based cleavable linker for quantitative proteomics.
abstract: Putting a number on it: Cleavable linkers are widely utilized in proteomics applications. In particular, the azobenzene-based linker cleaves under mild conditions that are mass-spectrometry-compatible. Here, we adapt this linker for quantitative proteomic applications by incorporating an isotopic label. These light- and heavy-tagged linkers enable the identification and quantitation of labeled peptides from multiple proteomes.
authors.affiliation: Department of Chemistry, Boston College, 2609 Beacon Street, Chestnut Hill, MA 02467, USA.
authors.forename: Yu
authors.initials: Y
authors.lastname: Qian
authors.forename: Julianne
authors.initials: J
authors.lastname: Martell
authors.forename: Nicholas J
authors.initials: NJ
authors.lastname: Pace
authors.forename: T Eric
authors.initials: TE
authors.lastname: Ballard
authors.forename: Douglas S
authors.initials: DS
authors.lastname: Johnson
authors.forename: Eranthie
authors.initials: E
authors.lastname: Weerapana
mesh_terms: D001391:Azo Compounds / Q000737:chemistry*; D057930:Click Chemistry; D003432:Cross-Linking Reagents / Q000737:chemistry; D057809:HEK293 Cells; D006801:Humans; D007202:Indicators and Reagents / Q000737:chemistry; D007553:Isotope Labeling; D015394:Molecular Structure; D010455:Peptides / Q000737:chemistry; D040901:Proteomics
chemical_list: D001391:Azo Compounds; D003432:Cross-Linking Reagents; D007202:Indicators and Reagents; D010455:Peptides; C009850:azobenzene
keywords: azobenzene; cleavable linkers; heavy isotopes; mass spectrometry; quantitative proteomics