title: Biomolecular membrane protein crystallization.
abstract: Integral membrane proteins comprise approximately 30% of the sequenced genomes, and there is an immediate need for their high-resolution structural information. Currently, the most reliable approach to obtain these structures is x-ray crystallography. However, obtaining crystals of membrane proteins that diffract to high resolution appears to be quite challenging, and remains a major obstacle in structural determination. This brief review summarizes a variety of methodologies for use in crystallizing these membrane proteins. Hopefully, by introducing the available methods, techniques, and providing a general understanding of membrane proteins, a rational decision can be made about now to crystallize these complex materials.
authors.affiliation: Department of Chemistry, Iowa State University, Ames, IA 50011, USA.
authors.forename: Jani Reddy
authors.initials: JR
authors.lastname: Bolla
authors.forename: Chih-Chia
authors.initials: CC
authors.lastname: Su
authors.forename: Edward W
authors.initials: EW
authors.lastname: Yu