title: Characterization of a large glycoprotein proteoglycan by size-exclusion chromatography combined with light and X-ray scattering methods.
abstract: The molecular weight and chain conformation of a proteoglycan derived from shark cartilage in solution were characterized by size-exclusion chromatography combined with low-angle laser light scattering and small-angle X-ray scattering methods. The total molecular weight of the proteoglycan was 3.9±0.2 million and the molecular weight of the main component was about 2.0±0.2 million. The X-ray scattering data revealed that the main components of the proteoglycan are nearly equal to a chain with excluded volume and their persistence lengths range from 13.5 to 16.4nm. These results show that size-exclusion chromatography combined with low-angle laser light scattering and small-angle X-ray scattering measurements are complementarily useful for characterization of large biopolymers in solution.
authors.affiliation: National Food Research Institute, NARO, 2-1-12 Kannondai, Tsukuba, Ibaraki 305-8642, Japan. yasuw@naro.affrc.go.jp
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Watanabe
authors.forename: Yoji
authors.initials: Y
authors.lastname: Inoko
mesh_terms: D058500:Animal Fins / Q000737:chemistry*; D000818:Animals; D002850:Chromatography, Gel / Q000379:methods*; D006023:Glycoproteins / Q000737:chemistry* / Q000302:isolation & purification*; D008970:Molecular Weight; D011509:Proteoglycans / Q000737:chemistry* / Q000302:isolation & purification*; D012542:Scattering, Radiation; D012754:Sharks
chemical_list: D006023:Glycoproteins; D011509:Proteoglycans
keywords: Conformation; Light scattering; Molecular weight; Proteoglycan; SEC-SAXS; Small-angle X-ray scattering