title: Distribution of infused umbilical cord mesenchymal stem cells in a rat model of renal interstitial fibrosis.
abstract: OBJECTIVE
  Stem cell transplantation for the treatment of kidney diseases is dependent on choice of transplant pathway. We evaluated the safety of human umbilical cord mesenchymal stem cells through peripheral infusion and their distribution in a rat model of renal interstitial fibrosis (RIF).
  
  
  METHODS
  Cryopreserved umbilical cord mesenchymal stem cells were infused via tail vein injection into rats with unilateral ureteral obstruction and Sham-operated. Blood, kidney, heart, liver, spleen and lung were collected at 14, 21, and 28 days after infusion. Testing included microscopic observation of kidney morphological changes and immunohistochemical testing to identify and count the number of MAB1281 (labeled human cells) positive cells in the heart, liver, spleen, lungs, and kidneys of different treatment groups.
  
  
  RESULTS
  There was no significant difference in the Sham-operated group and Sham-operated + cell transplantation group at different time points. Human cells were identified mainly in the lungs, spleen, and kidney. The number of human umbilical cord mesenchymal stem cells in the kidney was greater in the unilateral ureteral obstruction + cell transplantation group, compared to the Sham-operated + cell transplantation group. human umbilical cord mesenchymal stem cells were mainly located in the interstitium of the left kidney. These results suggest that infused mesenchymal stem cells were primed to engraft a damaged kidney, especially damaged renal interstitium.
  
  
  CONCLUSIONS
  Intravenous infusion of exogenous umbilical cord mesenchymal stem cells is feasible and safe. Infused mesenchymal stem cells can reach damaged kidney tissues with obstructive RIF after a vein graft.
authors.affiliation: Division of Pediatric Nephrology, Pediatric Medical Center, The Second Xiangya Hospital of Central South University, Changsha, Hunan, PR China. danlinhuang@yahoo.com
authors.forename: Danlin
authors.initials: D
authors.lastname: Huang
authors.forename: Zhuwen
authors.initials: Z
authors.lastname: Yi
authors.forename: Xiaojie
authors.initials: X
authors.lastname: He
authors.forename: Shuanghong
authors.initials: S
authors.lastname: Mo
authors.forename: Xiqiang
authors.initials: X
authors.lastname: Dang
authors.forename: Xiaochuan
authors.initials: X
authors.lastname: Wu
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D005240:Feasibility Studies; D006801:Humans; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000473:pathology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D051436:Renal Insufficiency, Chronic / Q000209:etiology / Q000628:therapy*; D014470:Umbilical Cord / Q000166:cytology*; D014517:Ureteral Obstruction / Q000209:etiology / Q000628:therapy*