title: Compound(s) secreted by Lactobacillus casei strain Shirota YIT9029 irreversibly and reversibly impair the swimming motility of Helicobacter pylori and Salmonella enterica serovar Typhimurium, respectively.
abstract: We conducted experiments in order to examine whether the probiotic Lactobacillus casei strain Shirota YIT9029 (LcS) in vitro and in vivo antagonism of Helicobacter pylori and Salmonella, involves inhibition of the swimming motility of these pathogens. We report the irreversible inhibition of the swimming motility of H. pylori strain 1101 and reversible inhibition of Salmonella enterica serovar Typhimurium (S. Typhimurium) strain SL1344 by compound(s) secreted by LcS. In H. pylori 1101, irreversible inhibition results in the helical cells being progressively replaced by cells with 'c'-shaped and coccoid morphologies, accompanied by a loss of FlaA and FlaB flagellin expression. In S. Typhimurium SL1344, transient inhibition develops after membrane depolarization and without modification of expression of FliC flagellin. The inhibitory activity of strain LcS against both S. Typhimurium and H. pylori swimming motilities is linked with a small sized, heat-sensitive, and partially trypsin-sensitive, secreted compound(s), and needed the cooperation of the secreted membrane permeabilizing lactic acid metabolite. The inhibition of S. Typhimurium SL1344 swimming motility leads to delayed cell entry into human enterocyte-like Caco-2/TC7 cells and a strong decrease of cell entry into human mucus-secreting HT29-MTX cells.
authors.affiliation: Faculté de Pharmacie, Université Paris-Sud, Châtenay-Malabry 92296, France.|UMR 8076 (BioCIS), CNRS, Faculté de Pharmacie, Université Paris-Sud, Châtenay-Malabry 92296, France.
authors.forename: Vanessa Liévin
authors.initials: VL
authors.lastname: Le Moal
authors.affiliation: Faculté de Pharmacie, Université Paris-Sud, Châtenay-Malabry 92296, France.|UMR 8076 (BioCIS), CNRS, Faculté de Pharmacie, Université Paris-Sud, Châtenay-Malabry 92296, France.
authors.forename: Domitille
authors.initials: D
authors.lastname: Fayol-Messaoudi
authors.affiliation: Faculté de Pharmacie, Université Paris-Sud, Châtenay-Malabry 92296, France.|UMR 8076 (BioCIS), CNRS, Faculté de Pharmacie, Université Paris-Sud, Châtenay-Malabry 92296, France.
authors.forename: Alain L
authors.initials: AL
authors.lastname: Servin
mesh_terms: D000898:Antibiosis; D001426:Bacterial Proteins / Q000378:metabolism; D001685:Biological Factors / Q000378:metabolism* / Q000494:pharmacology; D002460:Cell Line; D003470:Culture Media / Q000737:chemistry / Q000378:metabolism; D005408:Flagellin / Q000378:metabolism; D016481:Helicobacter Infections / Q000382:microbiology*; D016480:Helicobacter pylori / Q000187:drug effects / Q000502:physiology*; D006801:Humans; D007780:Lacticaseibacillus casei / Q000737:chemistry / Q000502:physiology*; D012480:Salmonella Infections / Q000382:microbiology*; D012486:Salmonella typhimurium / Q000187:drug effects / Q000502:physiology*
chemical_list: D001426:Bacterial Proteins; D001685:Biological Factors; D003470:Culture Media; D005408:Flagellin