title: Photoautotrophic outdoor two-stage cultivation for oleaginous microalgae Scenedesmus obtusus XJ-15.
abstract: In this study, Scenedesmus obtusus XJ-15 was firstly selected from seven strains microalgae (Chlorophyta, Scenedesmaceae) and then cultivated using a two-stage strategy, which composed of fast cell growth in stage I and followed by lipid induction in stage II in 5-L flasks outdoors. In stage I, the biomass productivity was increased from 139.4 to 212.1 mg L(-1) d(-1). In stage II, lipid content was increased from 26.1% to 47.7% by adding NaCl into the culture. This two-stage process was also realized in an 140-L photobioreactor outdoors, with a biomass productivity of 86.5 mg L(-1) d(-1) and CO2 fixation rate of 170.0 mg L(-1) d(-1) in the first stage, and high lipid content of 42.1% in the second stage. With such a culture strategy, the overall lipid productivity was improved and better biodiesel quality was obtained. These results suggested the photoautotrophic two-stage system was not only feasible but also effective.
authors.affiliation: Key Laboratory of Algal Biology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Xia
authors.forename: Hongmei
authors.initials: H
authors.lastname: Ge
authors.forename: Xuping
authors.initials: X
authors.lastname: Zhou
authors.forename: Delu
authors.initials: D
authors.lastname: Zhang
authors.forename: Chunxiang
authors.initials: C
authors.lastname: Hu
mesh_terms: D052818:Autotrophic Processes; D056804:Biofuels; D018533:Biomass; D002245:Carbon Dioxide / Q000378:metabolism; D018929:Cell Culture Techniques / Q000379:methods*; D003470:Culture Media / Q000494:pharmacology; D005227:Fatty Acids / Q000096:biosynthesis; D005240:Feasibility Studies; D008055:Lipids / Q000096:biosynthesis*; D058086:Microalgae / Q000254:growth & development* / Q000378:metabolism; D058433:Photobioreactors / Q000382:microbiology; D052817:Phototrophic Processes; D044444:Scenedesmus / Q000254:growth & development* / Q000378:metabolism; D013696:Temperature; D013997:Time Factors
chemical_list: D056804:Biofuels; D003470:Culture Media; D005227:Fatty Acids; D008055:Lipids; D002245:Carbon Dioxide
keywords: Biodiesel; Lipid induction; Outdoors; Photoautotrophic two-stage cultivation; Scenedesmus obtusus XJ-15