title: SIRT1 gene polymorphisms are associated with growth traits in Nanyang cattle.
abstract: Growth is under complex genetic control and uncovering the molecular mechanisms how the genes and polymorphisms affect economic growth traits, are important for successful marker-assisted selection and more efficient management strategies in commercial cattle populations. SIRT1 is a NAD(+)-dependent deacetylase that belongs to the class III histone deacetylases. It plays an important role in numerous fundamental cellular processes including gene silencing, DNA repair, and metabolic regulation. In addition, SIRT1 acts as an inhibitor of adipogenesis and has been associated with body weight regulation. The objective of the present study was to identify single nucleotide polymorphisms (SNPs) of bovine SIRT1 using 1255 animals representing the five main Chinese breeds and to determine if these SNPs are associated with economically important traits in Nanyang cattle. The approach consisted of resequencing SIRT1 using a panel of DNA from unrelated animals of five different breeds and the process revealed five novel SNPs. SNPs g.17324T>C and g.17491G>A exhibited a high degree of linkage disequilibrium in all tested breeds. Seven major haplotypes accounting for 91.2% of the alleles were observed and the haplotype 'GCCGA' was the most common haplotype in NY, QC, LX and JX breeds. An association analysis was performed between the five SNPs and six performance traits. SNP g.-274C>G was demonstrated to have a strong effect on 24-months-old body weight and g.17379A>G polymorphism was related to 6 and 12-months-old body weight in NY population, although these effects did not remained significant after the Bonferroni correction. Our results provide evidence that polymorphisms in SIRT1 are associated with growth efficiency traits, and may be used for marker-assisted selection and management in feedlot cattle.
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, No. 22 Xinong Road, Yangling, Shaanxi 712100, China. Electronic address: limingxun@live.com.
authors.forename: Mingxun
authors.initials: M
authors.lastname: Li
authors.forename: Xiaomei
authors.initials: X
authors.lastname: Sun
authors.forename: Liushuai
authors.initials: L
authors.lastname: Hua
authors.forename: Xinsheng
authors.initials: X
authors.lastname: Lai
authors.forename: Xianyong
authors.initials: X
authors.lastname: Lan
authors.forename: Chuzhao
authors.initials: C
authors.lastname: Lei
authors.forename: Chunlei
authors.initials: C
authors.lastname: Zhang
authors.forename: Xinglei
authors.initials: X
authors.lastname: Qi
authors.forename: Hong
authors.initials: H
authors.lastname: Chen
mesh_terms: D000483:Alleles; D000818:Animals; D001483:Base Sequence; D001835:Body Weight / Q000235:genetics*; D002417:Cattle / Q000235:genetics* / Q000254:growth & development*; D056726:Genetic Association Studies; D014644:Genetic Variation; D005838:Genotype; D006239:Haplotypes; D015810:Linkage Disequilibrium; D008969:Molecular Sequence Data; D010641:Phenotype; D020641:Polymorphism, Single Nucleotide; D056564:Sirtuin 1 / Q000235:genetics*
chemical_list: D056564:Sirtuin 1
keywords: Association; Cattle; Growth traits; Haplotype; SIRT1; SNPs