title: Hepatoprotective and antioxidant effects of saponarin, isolated from Gypsophila trichotoma Wend. on paracetamol-induced liver damage in rats.
abstract: The hepatoprotective potential of saponarin, isolated from Gypsophila trichotoma, was evaluated in vitro/in vivo using a hepatotoxicity model of paracetamol-induced liver injury. In freshly isolated rat hepatocytes, paracetamol (100 μ mol) led to a significant decrease in cell viability, increased LDH leakage, decreased levels of cellular GSH, and elevated MDA quantity. Saponarin (60-0.006 μ g/mL) preincubation, however, significantly ameliorated paracetamol-induced hepatotoxicity in a concentration-dependent manner. The beneficial effect of saponarin was also observed in vivo. Rats were challenged with paracetamol alone (600 mg/kg, i.p.) and after 7-day pretreatment with saponarin (80 mg/kg, oral gavage). Paracetamol toxicity was evidenced by increase in MDA quantity and decrease in cell GSH levels and antioxidant defence system. No changes in phase I enzyme activities of AH and EMND and cytochrome P 450 quantity were detected. Saponarin pretreatment resulted in significant increase in cell antioxidant defence system and GSH levels and decrease in lipid peroxidation. The biochemical changes are in good correlation with the histopathological data. Protective activity of saponarin was similar to the activity of positive control silymarin. On the basis of these results, it can be concluded that saponarin exerts antioxidant and hepatoprotective activity against paracetamol liver injury in vitro/in vivo.
authors.affiliation: Laboratory of Drug Metabolism and Drug Toxicity, Department of Pharmacology, Pharmacotherapy and Toxicology, Faculty of Pharmacy, Medical University, 2 Dunav Street, 1000 Sofia, Bulgaria.
authors.forename: Rumyana
authors.initials: R
authors.lastname: Simeonova
authors.forename: Vessela
authors.initials: V
authors.lastname: Vitcheva
authors.forename: Magdalena
authors.initials: M
authors.lastname: Kondeva-Burdina
authors.forename: Ilina
authors.initials: I
authors.lastname: Krasteva
authors.forename: Vassil
authors.initials: V
authors.lastname: Manov
authors.forename: Mitka
authors.initials: M
authors.lastname: Mitcheva
mesh_terms: D000082:Acetaminophen; D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage*; D047310:Apigenin / Q000008:administration & dosage*; D001487:Basidiomycota / Q000378:metabolism*; D029748:Caryophyllaceae / Q000378:metabolism*; D056486:Chemical and Drug Induced Liver Injury / Q000209:etiology / Q000378:metabolism* / Q000517:prevention & control*; D019610:Cytoprotection; D004305:Dose-Response Relationship, Drug; D005960:Glucosides / Q000008:administration & dosage*; D008297:Male; D010936:Plant Extracts / Q000008:administration & dosage; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000975:Antioxidants; D005960:Glucosides; D010936:Plant Extracts; C457771:saponarin; D000082:Acetaminophen; D047310:Apigenin