title: Sensitive ECL immunosensor for detection of retinol-binding protein based on double-assisted signal amplification strategy of multiwalled carbon nanotubes and Ru(bpy)3(2+) doped mesoporous silica nanospheres.
abstract: A novel electrochemiluminescence (ECL) strategy based on the sandwich-type immunosensor for sensitive detection of retinol-binding protein (RBP) was developed. The primary antibody anti-RBP was immobilized onto multiwalled carbon nanotubes (MWCNTs), which have large surface area and high electrical conductivity. The RBP antigen and Ru-Nafion@SiO2-labeled secondary antibody were then successively conjugated to form sandwich-type immunocomplexes through the specific interaction between antigen and antibody. The ECL signal amplification was significantly improved due to the synergistic effect of MWCNTs and mesoporous silica nanospheres (mSiO2). The developed ECL immunosensor exhibited high sensitivity and specificity for the detection of RBP and responded linearly to the clinically-relevant concentration of RBP from 78 to 5000 ng mL(-1). Moreover, the MWCNT-based ECL immunosensor displayed excellent stability and reproducibility, as well as successfully achieved the detection of RBP in patient urine samples with desirable results. The present work provided a promising technique for the clinical screening of RBP and point-of-care diagnostics.
authors.affiliation: The Education Ministry Key Laboratory of Resource Chemistry and Shanghai Key Laboratory of Rare Earth Functional Materials, Department of Chemistry, College of Life and Environmental Sciences, Shanghai Normal University, Shanghai 200234, PR China.
authors.forename: Beina
authors.initials: B
authors.lastname: Wu
authors.forename: Chenyi
authors.initials: C
authors.lastname: Hu
authors.forename: Xiaoqing
authors.initials: X
authors.lastname: Hu
authors.forename: Hongmei
authors.initials: H
authors.lastname: Cao
authors.forename: Chusen
authors.initials: C
authors.lastname: Huang
authors.forename: Hebai
authors.initials: H
authors.lastname: Shen
authors.forename: Nengqin
authors.initials: N
authors.lastname: Jia
mesh_terms: D015082:2,2'-Dipyridyl / Q000031:analogs & derivatives* / Q000737:chemistry; D055910:Antibodies, Immobilized / Q000737:chemistry*; D015374:Biosensing Techniques / Q000379:methods*; D056831:Coordination Complexes; D006801:Humans; D057230:Limit of Detection; D008163:Luminescent Measurements / Q000379:methods*; D037742:Nanotubes, Carbon / Q000737:chemistry*; D016062:Porosity; D012177:Retinol-Binding Proteins / Q000032:analysis / Q000652:urine*; D012822:Silicon Dioxide / Q000737:chemistry; D017975:Ruthenium Compounds
chemical_list: D015082:2,2'-Dipyridyl; D055910:Antibodies, Immobilized; D056831:Coordination Complexes; D037742:Nanotubes, Carbon; D012177:Retinol-Binding Proteins; D012822:Silicon Dioxide; C547232:tris(2,2-bipyridine)-ruthenium(II); D017975:Ruthenium Compounds
keywords: Biosensors; Clinical diagnostics; Electrochemiluminescence; Mesoporous silica materials; Retinol-binding protein