title: Enhanced induction of cell cycle arrest and apoptosis via the mitochondrial membrane potential disruption in human U87 malignant glioma cells by aloe emodin.
abstract: Aloe emodin, one of the active compounds found in Aloe vera leaves, plays an important role in the regulation of cell growth and death. It has been reported to promote the anti-cancer effects in various cancer cells by inducing apoptosis. However, the mechanism of inducing apoptosis by this agent is poorly understood in glioma cells. This research is to investigate the apoptosis and cell cycle arrest inducing by aloe emodin on U87 human malignant glioma cells. Aloe emodin showed a time- and dose-dependent inhibition of U87 cells proliferation and decreased the percentage of viable U87 cells via the induction of apoptosis. Characteristic morphological changes, such as the formation of apoptotic bodies, were observed with confocal microscope by Annexin V-FITC/PI staining, supporting our viability study and flow cytometry analysis results. Our data also demonstrated that aloe emodin arrested the cell cycle in the S phase and promoted the loss of mitochondrial membrane potential in U87 cells that indicated the early event of the mitochondria-induced apoptotic pathway.
authors.affiliation: a Department of Neurosciences , Universiti Sains Malaysia , Kubang Kerian Kelantan 16150 , Malaysia.
authors.forename: Samhani
authors.initials: S
authors.lastname: Ismail
authors.forename: Khalilah
authors.initials: K
authors.lastname: Haris
authors.forename: Abdul Rahman Izaini
authors.initials: AR
authors.lastname: Abdul Ghani
authors.forename: Jafri Malin
authors.initials: JM
authors.lastname: Abdullah
authors.forename: Muhammad Farid
authors.initials: MF
authors.lastname: Johan
authors.forename: Abdul Aziz
authors.initials: AA
authors.lastname: Mohamed Yusoff
mesh_terms: D000504:Aloe / Q000737:chemistry*; D000880:Anthraquinones / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D002453:Cell Cycle / Q000187:drug effects*; D059447:Cell Cycle Checkpoints; D049109:Cell Proliferation / Q000187:drug effects; D005910:Glioma; D006801:Humans; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D015394:Molecular Structure; D018515:Plant Leaves / Q000737:chemistry
chemical_list: D000880:Anthraquinones; C518327:aloe emodin