title: Low expression of dendritic cell-specific intercellular adhesion molecule-grabbing nonintegrin-related protein in non-Hodgkin lymphoma and significant correlations with lactic acid dehydrogenase and β2-microglobulin.
abstract: Dendritic cell-specific intercellular adhesion molecule-grabbing nonintegrin-related protein (DC-SIGNR), a type II integral membrane protein and a member of the C-type lectins, has been reported to bind various strains of HIV-1, HIV-2, and simian immunodeficiency virus. Serum DC-SIGNR is not currently available for the detection of non-Hodgkin lymphoma (NHL). Using an enzyme-linked immunosorbent assay (ELISA), we assessed the serum levels of DC-SIGNR in 70 cancer patients and 100 healthy controls. Additionally, using immunohistochemistry, we determined the expression of DC-SIGNR in the lymph nodes. Using the ELISA, low serum levels of DC-SIGNR were detected in the patients (median, 4.513 ng·L(-1); range, 1.066-9.232 ng·L(-1); p = 0.0003). Serum concentrations of DC-SIGNR correlated significantly with age (p = 0.0077) and lactic acid dehydrogenase (p = 0.0046) and β2-microglobulin (p = 0.0491) levels. However, we found no statistically significant correlation between serum DC-SIGNR levels and clinical data such as sex, Ann Arbor stage, B symptoms, and histologic subtypes. Moreover, NHL patients with a lower level of serum DC-SIGNR expression in lymphatic endothelial cells also showed negative immunostaining levels. These results suggest that DC-SIGNR is a biological molecule that may be potentially useful in NHL clinical settings.
authors.affiliation: Department of Clinical Biochemistry, College of Laboratory Diagnostic Medicine, Dalian Medical University, Dalian 116044, China.
authors.forename: Zhuqing
authors.initials: Z
authors.lastname: Zhang
authors.forename: Kai
authors.initials: K
authors.lastname: Chen
authors.forename: Longchao
authors.initials: L
authors.lastname: Yan
authors.forename: Ziyi
authors.initials: Z
authors.lastname: Yang
authors.forename: Zhanmeng
authors.initials: Z
authors.lastname: Zhu
authors.forename: Chunlin
authors.initials: C
authors.lastname: Chen
authors.forename: Jia
authors.initials: J
authors.lastname: Zeng
authors.forename: Wei
authors.initials: W
authors.lastname: Wei
authors.forename: Xia
authors.initials: X
authors.lastname: Qi
authors.forename: Shuangyi
authors.initials: S
authors.lastname: Ren
authors.forename: Yunfei
authors.initials: Y
authors.lastname: Zuo
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002265:Carboxylic Ester Hydrolases / Q000378:metabolism*; D002448:Cell Adhesion; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D003713:Dendritic Cells / Q000166:cytology* / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D016023:Integrins / Q000378:metabolism*; D037181:Lectins, C-Type / Q000378:metabolism*; D008198:Lymph Nodes / Q000378:metabolism; D008228:Lymphoma, Non-Hodgkin / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D011956:Receptors, Cell Surface / Q000378:metabolism*; D055815:Young Adult; D001613:beta 2-Microglobulin / Q000378:metabolism*
chemical_list: C433029:CLEC4M protein, human; D015815:Cell Adhesion Molecules; D016023:Integrins; D037181:Lectins, C-Type; D011956:Receptors, Cell Surface; D001613:beta 2-Microglobulin; D002265:Carboxylic Ester Hydrolases; C425772:poly(lactic acid) depolymerase