title: Detailed modulation of phenotypes and functions of bone marrow dendritic cells (BMDCs) by interferon-gamma (IFN-γ).
abstract: IFN-γ is a cytokine that plays crucial role in innate and adaptive immunity against viral and intracellular bacterial infections and for tumor control. IFN-γ is also a key activator of macrophages [1,2]. In the present study, we studied detailed modulation of IFN-γ on phenotypic and functional maturation of murine bone marrow derived dendritic cells (BMDCs). Phenotypic and functional maturation of BMDCs was evaluated by light microscope, flow cytometry(FCM), transmission electron microscopy (TEM), cytochemistry method, acid phosphatase activity(ACP), FITC-dextran bio-assay and enzyme linked immunosorbent assay (ELISA). We elucidated that IFN-γ up-regulated the expression of MHC II, CD40, CD80, CD83 and CD86 molecules on BMDCs, down-regulated the activity of pinocytosis and phagocytosis by BMDCs, and induced higher levels of IL-12 and TNF-α secreted by BMDCs. It is therefore confirmed that IFN-γ can effectively promote the maturation of BMDCs. Our study provides more evidence and rationale on future application of IFN-γ for enhancing host immunity.
authors.affiliation: Department of Endodontics, China Medical University, Shenyang, People's Republic of China. alex_xm@sina.com
authors.forename: Ming
authors.initials: M
authors.lastname: Xue
authors.forename: Linlin
authors.initials: L
authors.lastname: Zhu
authors.forename: Yiming
authors.initials: Y
authors.lastname: Meng
authors.forename: Li
authors.initials: L
authors.lastname: Wang
authors.forename: Haiyan
authors.initials: H
authors.lastname: Sun
authors.forename: Fen
authors.initials: F
authors.lastname: Wang
authors.forename: Enhu
authors.initials: E
authors.lastname: Wang
authors.forename: Fengping
authors.initials: F
authors.lastname: Shan
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D001854:Bone Marrow Cells / Q000276:immunology*; D002454:Cell Differentiation / Q000187:drug effects; D002469:Cell Separation; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000276:immunology*; D005260:Female; D005434:Flow Cytometry; D000949:Histocompatibility Antigens Class II / Q000378:metabolism; D016130:Immunophenotyping; D007371:Interferon-gamma / Q000494:pharmacology*; D007376:Interleukin-2 / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D010587:Phagocytosis / Q000187:drug effects; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D015703:Antigens, CD; D000949:Histocompatibility Antigens Class II; D007376:Interleukin-2; D014409:Tumor Necrosis Factor-alpha; D007371:Interferon-gamma
keywords: 3, 3′-diaminobenzidine; 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; ACP; APCs; BMDCs; Bone marrow derived dendritic cells; CTL; DAB; FCM; GM-CSF; IFN-γ; IL-4; Immune system; Interferon-gamma; LPS; MACS; MTT; Maturation; Modulation; TEM; acidic phosphatase; antigen presenting cells; bone marrow derived dendritic cells; cytotoxic lymphocyte; flow cytometry; granulocyte macrophage colony stimulating factor; interferon-gamma; interleukin-4; lipopolysaccharide; magnetic activated cell sorting; transmission electron microscopy