title: Fluoroquinolone levels in healthy dog urine following a 20-mg/kg oral dose of enrofloxacin exceed mutant prevention concentration targets against Escherichia coli isolated from canine urinary tract infections.
abstract: A 3-day course of oral enrofloxacin is effective for treating uncomplicated urinary tract infection (UTI) in dogs when administered 20 mg/kg Q24H. However, emergence of fluoroquinolone-resistant mutants of uropathogens is a concern. Urine concentrations of enrofloxacin and ciprofloxacin were measured in six healthy dogs following dose of enrofloxacin 20 mg/kg. Mutant prevention concentrations of Escherichia coli isolated from canine UTI were also determined against ciprofloxacin. Urine AUC(24)/MPC ratios considering ciprofloxacin concentrations ranged 3819-7767, indicating that selection of resistant E. coli mutants in dogs with uncomplicated UTIs is unlikely in the bladder given that an AUC(24)/MPC = 39 is considered to be protective against mutant selection for ciprofloxacin. However, additional studies are required to evaluate the effects of this enrofloxacin treatment protocol on bacteria that colonize anatomic sites where fluoroquinolones achieve lower concentrations compared to the urinary bladder.
authors.affiliation: Department of Veterinary Clinical Sciences, The Ohio State University College of Veterinary Medicine, Columbus, OH, USA.
authors.forename: J B
authors.initials: JB
authors.lastname: Daniels
authors.forename: G
authors.initials: G
authors.lastname: Tracy
authors.forename: S J
authors.initials: SJ
authors.lastname: Irom
authors.forename: J
authors.initials: J
authors.lastname: Lakritz
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000493:pharmacokinetics* / Q000652:urine*; D002939:Ciprofloxacin / Q000378:metabolism / Q000652:urine; D004283:Dog Diseases / Q000382:microbiology; D004285:Dogs / Q000652:urine*; D024881:Drug Resistance, Bacterial; D000077422:Enrofloxacin; D004926:Escherichia coli / Q000187:drug effects*; D004927:Escherichia coli Infections / Q000662:veterinary; D024841:Fluoroquinolones / Q000378:metabolism / Q000493:pharmacokinetics* / Q000652:urine*; D009154:Mutation; D014552:Urinary Tract Infections / Q000382:microbiology / Q000662:veterinary
chemical_list: D000970:Antineoplastic Agents; D024841:Fluoroquinolones; D000077422:Enrofloxacin; D002939:Ciprofloxacin