title: MicroRNA-27a is induced by leucine and contributes to leucine-induced proliferation promotion in C2C12 cells.
abstract: Leucine, a branched chain amino acid, is well known to stimulate protein synthesis in skeletal muscle. However, the role of leucine in myoblast proliferation remains unclear. In this study, we found that leucine could promote proliferation of C2C12 cells. Moreover, expressions of miR-27a and myostatin (a bona fide target of miR-27a) were upregulated and downregulated, respectively, following leucine treatment. We also found that miR-27a loss-of-function by transfection of a miR-27a inhibitor suppressed the promotion of myoblast proliferation caused by leucine. Our results suggest that miR-27a is induced by leucine and contributes to leucine-induced proliferation promotion of myoblast.
authors.affiliation: Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu 611130, Sichuan, China. xlchen@sicau.edu.cn
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Chen
authors.forename: Zhiqing
authors.initials: Z
authors.lastname: Huang
authors.forename: Daiwen
authors.initials: D
authors.lastname: Chen
authors.forename: Ting
authors.initials: T
authors.lastname: Yang
authors.forename: Guangmang
authors.initials: G
authors.lastname: Liu
mesh_terms: D000818:Animals; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D007930:Leucine / Q000494:pharmacology*; D051379:Mice; D035683:MicroRNAs / Q000037:antagonists & inhibitors / Q000378:metabolism*; D032446:Myoblasts / Q000166:cytology / Q000378:metabolism; D055435:Myostatin / Q000235:genetics / Q000378:metabolism; D015854:Up-Regulation / Q000187:drug effects*
chemical_list: D035683:MicroRNAs; C540674:Mirn27 microRNA, mouse; D055435:Myostatin; D007930:Leucine