title: Isolation, characterization, and transplantation of bone marrow-derived cell components with hematopoietic stem cell niche properties.
abstract: Although the unique role of hematopoietic stem cell (HSC) niche in hematopoiesis has long been recognized, unsuccessful isolation of intact niche units limited their in vitro study, manipulation, and therapeutic application. Here, we isolated cell complexes based on size fractionation from mouse bone marrow (BM), characterized the derived cells, and transplanted them to irradiated mice. These cell complexes were the origin of both BM mesenchymal stem cells and various hematopoietic lineages when kept in appropriate culture conditions. They also had the potential of recruiting circulating HSC. Intraperitoneal transplantation of these structures into irradiated mice not only showed long-lasting hematopoietic multilineage reconstitution, but also could recover the stromal cells of BM. In conclusion, this study for the first time provides evidences on the feasibility and efficacy of transplantation of HSC in association with their native specialized microenvironment. As the molecular cross-talk between HSC and niche is crucial for their proper function, the proposed method could be considered as a novel hematopoietic transplantation strategy.
authors.affiliation: 1 Department of Hematology, Tarbiat Modares University , Tehran, Iran .
authors.forename: Naser
authors.initials: N
authors.lastname: Ahmadbeigi
authors.forename: Masoud
authors.initials: M
authors.lastname: Soleimani
authors.forename: Mohammad
authors.initials: M
authors.lastname: Vasei
authors.forename: Yousof
authors.initials: Y
authors.lastname: Gheisari
authors.forename: Yousef
authors.initials: Y
authors.lastname: Mortazavi
authors.forename: Kayhan
authors.initials: K
authors.lastname: Azadmanesh
authors.forename: Azadeh
authors.initials: A
authors.lastname: Omidkhoda
authors.forename: Ehsan
authors.initials: E
authors.lastname: Janzamin
authors.forename: Nance Beyer
authors.initials: NB
authors.lastname: Nardi
mesh_terms: D034841:Abdominal Cavity / Q000502:physiology; D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology*; D016026:Bone Marrow Transplantation; D002469:Cell Separation / Q000379:methods*; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006413:Hematopoietic System / Q000166:cytology; D008198:Lymph Nodes / Q000166:cytology; D051379:Mice; D008810:Mice, Inbred C57BL; D055153:Stem Cell Niche; D017154:Stromal Cells / Q000166:cytology