title: CMV amplifies T-cell redeployment to acute exercise independently of HSV-1 serostatus.
abstract: OBJECTIVE
  Latent cytomegalovirus (CMV) infection has been shown to alter the lymphocyte response to acute aerobic exercise, likely due to the corresponding increase in exercise-responsive memory CD8(+) T cells. It is unknown if latent infection with another herpesvirus, herpes simplex virus 1 (HSV-1), also plays a role in shaping the lymphocyte response to exercise.
  
  
  METHODS
  Thirty-two men (ages 39.3 ± 14.7 yr) counterbalanced by CMV and HSV-1 serostatus (positive/negative) cycled for 30 min at ∼80% peak power. Blood sampled before, immediately after, and 1 h after exercise was analyzed by flow cytometry for T-cell subset enumeration.
  
  
  RESULTS
  In resting blood, HSV-1(+) had fewer lymphocytes, CD4(+) T cells, KLRG1(-) CD28(+) CD4(+) T cells, and CD45RA(-)CCR7(+)CD4(+) T cells than HSV-1(-), whereas CMV(+) had increased numbers of lymphocytes, CD8(+) T cells, KLRG1(+)CD28(-)CD4(+) and CD8(+) T cells, and CD45RA(+)CCR7(-)CD8(+) T cells and a lower CD4:CD8 T-cell ratio than CMV(-). After exercise, CMV(+) had a greater mobilization of CD8(+) T cells, KLRG1+CD28(-)CD4+ and CD8(+) T cells, and CD45RA+CCR7(-)CD8+ T cells independently of HSV-1 serostatus, as well as a greater egress of these subsets 1 h after exercise. HSV serostatus did not influence total CD8(+) T-cell response to exercise.
  
  
  CONCLUSIONS
  The impact of latent CMV infection on the redeployment of T-cell subsets with exercise is independent of HSV-1 infection. This is most likely due to the unique ability of CMV to alter the composition of the memory T-cell pool in favor of exercise-responsive T-cell subsets.
authors.affiliation: Laboratory of Integrated Physiology, Department of Health and Human Performance, University of Houston, Houston, TX.
authors.forename: Emily C
authors.initials: EC
authors.lastname: Lavoy
authors.forename: Austin B
authors.initials: AB
authors.lastname: Bigley
authors.forename: Guillaume
authors.initials: G
authors.lastname: Spielmann
authors.forename: Jerrald L
authors.initials: JL
authors.lastname: Rector
authors.forename: Mark R
authors.initials: MR
authors.lastname: Morrison
authors.forename: Daniel P
authors.initials: DP
authors.lastname: O'Connor
authors.forename: Richard J
authors.initials: RJ
authors.lastname: Simpson
mesh_terms: D000328:Adult; D018106:CD28 Antigens / Q000032:analysis; D017252:CD3 Complex / Q000032:analysis; D016516:CD4-CD8 Ratio; D015496:CD4-Positive T-Lymphocytes / Q000737:chemistry; D018414:CD8-Positive T-Lymphocytes / Q000737:chemistry; D060085:Coinfection / Q000276:immunology*; D003586:Cytomegalovirus Infections / Q000150:complications / Q000276:immunology*; D015444:Exercise / Q000502:physiology*; D006561:Herpes Simplex / Q000150:complications / Q000276:immunology* / Q000821:virology; D018259:Herpesvirus 1, Human; D006801:Humans; D037181:Lectins, C-Type / Q000032:analysis; D008297:Male; D008875:Middle Aged; D011971:Receptors, Immunologic; D012698:Serologic Tests; D016176:T-Lymphocyte Subsets; D015534:Trans-Activators / Q000032:analysis; D055815:Young Adult
chemical_list: D018106:CD28 Antigens; D017252:CD3 Complex; C113413:KLRG1 protein, human; D037181:Lectins, C-Type; D011971:Receptors, Immunologic; D015534:Trans-Activators