title: Heptachlor induced mitochondria-mediated cell death via impairing electron transport chain complex III.
abstract: Environmental toxins like pesticides have been implicated in the pathogenesis of Parkinson's disease (PD). Epidemiological studies suggested that exposures to organochlorine pesticides have an association with an increased PD risk. In the present study, we examined the mechanism of toxicity induced by an organochlorine pesticide heptachlor. In a human dopaminergic neuroblastoma SH-SY5Y cells, heptachlor induced both morphological and functional damages in mitochondria. Interestingly, the compound inhibited mitochondrial electron transport chain complex III activity. Rapid generation of reactive oxygen species and the activation of Bax were then detected. Subsequently, mitochondria-mediated, caspase-dependent apoptosis followed. Our results raise a possibility that an organochlorine pesticide heptachlor can act as a neurotoxicant associated with PD.
authors.affiliation: Department of Molecular Biology, Sejong University, Seoul, Republic of Korea.
authors.forename: Seokheon
authors.initials: S
authors.lastname: Hong
authors.forename: Joo Yeon
authors.initials: JY
authors.lastname: Kim
authors.forename: Joohyun
authors.initials: J
authors.lastname: Hwang
authors.forename: Ki Soon
authors.initials: KS
authors.lastname: Shin
authors.forename: Shin Jung
authors.initials: SJ
authors.lastname: Kang
mesh_terms: D017209:Apoptosis; D020169:Caspases / Q000378:metabolism; D045744:Cell Line, Tumor; D059290:Dopaminergic Neurons / Q000378:metabolism; D014450:Electron Transport Complex III / Q000187:drug effects / Q000378:metabolism*; D006533:Heptachlor / Q000494:pharmacology*; D006801:Humans; D007306:Insecticides / Q000494:pharmacology*; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism*; D009447:Neuroblastoma / Q000378:metabolism / Q000473:pathology; D017382:Reactive Oxygen Species / Q000378:metabolism; D051028:bcl-2-Associated X Protein / Q000378:metabolism
chemical_list: D007306:Insecticides; D017382:Reactive Oxygen Species; D051028:bcl-2-Associated X Protein; D006533:Heptachlor; D020169:Caspases; D014450:Electron Transport Complex III
keywords: Apoptosis; Heptachlor; Mitochondria; Parkinson’s disease; Pesticide; Reactive oxygen species