title: Effects of pathogenic bacterial challenge after acute sublethal ammonia-N exposure on heat shock protein 70 expression in Botia reevesae.
abstract: The objective of this study was to investigate the effects of pathogenic bacterial challenge after acute sublethal ammonia-N exposure on heat shock protein 70 expression in Botia reevesae. After ammonia-N exposure at a constant concentration of 7.21 ± 0.10 mg L(-1) for 96 h, B. reevesae was challenged with Aeromonas hydrophila. Quantitative PCR analysis showed predominant and significant expression of HSP70 in liver, gill, skin, spleen and kidney (P < 0.05), with significantly upregulated expression of the mRNA transcript in these tissues after sublethal ammonia-N exposure and A. hydrophila challenge. Furthermore, following A. hydrophila challenge after ammonia-N exposure, HSP70 mRNA expression was significantly upregulated in kidney and gill tissues, although its expression levels were significantly lower than those detected following A. hydrophila challenge or ammonia-N exposure individually. These results indicate that B. reevesae HSP70 is involved in resistance to pathogenic bacteria. It is hypothesized that ammonia-N results in the downregulation of HSP70 mRNA in immune organs after an A. hydrophila challenge, thus lowering their resistance to pathogenic stress.
authors.affiliation: College of Life Science, Neijiang Normal University, Neijiang 641112, PR China.
authors.forename: Chuanjie
authors.initials: C
authors.lastname: Qin
authors.forename: Daxian
authors.initials: D
authors.lastname: Zhao
authors.forename: Quan
authors.initials: Q
authors.lastname: Gong
authors.forename: Zemin
authors.initials: Z
authors.lastname: Qi
authors.forename: Yuanchao
authors.initials: Y
authors.lastname: Zou
authors.forename: Xingjian
authors.initials: X
authors.lastname: Yue
authors.forename: Biwen
authors.initials: B
authors.lastname: Xie
mesh_terms: D016980:Aeromonas hydrophila; D000641:Ammonia / Q000008:administration & dosage / Q000633:toxicity*; D000818:Animals; D003531:Cypriniformes; D005393:Fish Diseases / Q000378:metabolism* / Q000382:microbiology; D005786:Gene Expression Regulation / Q000276:immunology*; D005880:Gills / Q000378:metabolism; D016905:Gram-Negative Bacterial Infections / Q000276:immunology / Q000382:microbiology / Q000662:veterinary*; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism*; D007668:Kidney / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013154:Spleen / Q000378:metabolism; D059467:Transcriptome; D014874:Water Pollutants, Chemical / Q000633:toxicity
chemical_list: D018840:HSP70 Heat-Shock Proteins; D012333:RNA, Messenger; D014874:Water Pollutants, Chemical; D000641:Ammonia
keywords: Aeromonas hydrophila; Ammonia-N; Botia reevesae; Heat shock protein 70