title: Proteolytic post-translational modification of proteins: proteomic tools and methodology.
abstract: Proteolytic processing is a ubiquitous and irreversible post-translational modification involving limited and highly specific hydrolysis of peptide and isopeptide bonds of a protein by a protease. Cleavage generates shorter protein chains displaying neo-N and -C termini, often with new or modified biological activities. Within the past decade, degradomics and terminomics have emerged as significant proteomics subfields dedicated to characterizing proteolysis products as well as natural protein N and C termini. Here we provide an overview of contemporary proteomics-based methods, including specific quantitation, data analysis, and curation considerations, and highlight exciting new and emerging applications within these fields enabling in vivo analysis of proteolytic events.
authors.affiliation: Department of Biochemistry and Molecular Biology, Department of Oral Biological and Medical Sciences, and Centre for Blood Research, University of British Columbia, 4.401 Life Sciences Institute, 2350 Health Sciences Mall, Vancouver, British Columbia, V6T 1Z3, Canada.
authors.forename: Lindsay D
authors.initials: LD
authors.lastname: Rogers
authors.forename: Christopher M
authors.initials: CM
authors.lastname: Overall
mesh_terms: D020816:Amino Acid Motifs; D019921:Biotinylation; D002853:Chromatography, Liquid; D020650:Combinatorial Chemistry Techniques / Q000379:methods*; D006801:Humans; D007553:Isotope Labeling; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry / Q000302:isolation & purification*; D011499:Protein Processing, Post-Translational; D017434:Protein Structure, Tertiary; D059748:Proteolysis; D020543:Proteome / Q000737:chemistry / Q000378:metabolism*; D040901:Proteomics / Q000295:instrumentation / Q000379:methods*; D013379:Substrate Specificity
chemical_list: D010446:Peptide Fragments; D020543:Proteome