title: Anti-Müllerian hormone (AMH), inhibin-α, growth differentiation factor 9 (GDF9), and bone morphogenic protein-15 (BMP15) mRNA and protein are influenced by photoperiod-induced ovarian regression and recrudescence in Siberian hamster ovaries.
abstract: Exposure of Siberian hamsters to short photoperiod (SD) inhibits ovarian function, including folliculogenesis, whereas function is restored with their transfer to long photoperiods (LD). To investigate the mechanism of photo-stimulated recrudescence, we assessed key folliculogenic factors-anti-Müllerian hormone (AMH), inhibin-α, growth differentiation factor-9 (GDF9), and bone morphogenic protein-15 (BMP15)-across the estrus cycle and in photo-regressed and recrudescing ovaries. Adult hamsters were exposed to either LD or SD for 14 weeks, which respectively represent functional and regressed ovaries. Select regressed hamsters were transferred back to LD for 2 (post-transfer week 2; PTw2) or 8 weeks (PTw8). Ovaries were collected and fixed in formalin for immunohistochemistry or frozen in liquid nitrogen for real-time PCR. AMH, inhibin-α, GDF9, and BMP15 mRNA and protein were detected in all stages of the estrus cycle. Fourteen weeks of SD exposure increased (P < 0.05) ovarian AMH, GDF9, and BMP15, but not inhibin-α mRNA levels as compared to LD. Transfer of regressed hamsters to stimulatory long photoperiod for 8 weeks returned AMH and GDF9 mRNA levels to LD-treated levels, and further increased mRNA levels for inhibin-α and BMP15. Immunostaining for AMH, inhibin-α, GDF9, and BMP15 proteins was most intense in preantral/antral follicles and oocytes. The overall immunostaining extent for AMH and inhibin-α generally mirrored the mRNA data, though no changes were observed for GDF9 or BMP15 immunostaining. Shifts in mRNA and protein levels across photoperiod conditions suggest possible syncretic roles for these folliculogenic factors in photo-stimulated recrudescence via potential regulation of follicle recruitment, preservation, and development.
authors.affiliation: Department of Biological Sciences, California State University Long Beach, Long Beach, California.
authors.forename: Asha
authors.initials: A
authors.lastname: Shahed
authors.forename: Kelly A
authors.initials: KA
authors.lastname: Young
mesh_terms: D000818:Animals; D054304:Anti-Mullerian Hormone / Q000032:analysis* / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D055430:Bone Morphogenetic Protein 15 / Q000032:analysis* / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D006224:Cricetinae; D004958:Estradiol / Q000097:blood; D005260:Female; D055429:Growth Differentiation Factor 9 / Q000032:analysis* / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D007265:Inhibins / Q000032:analysis* / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D010053:Ovary / Q000737:chemistry / Q000378:metabolism / Q000528:radiation effects; D017111:Phodopus; D017440:Photoperiod; D012333:RNA, Messenger / Q000032:analysis* / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D012008:Recurrence
chemical_list: D055430:Bone Morphogenetic Protein 15; D055429:Growth Differentiation Factor 9; D012333:RNA, Messenger; C094377:inhibin-alpha subunit; D004958:Estradiol; D007265:Inhibins; D054304:Anti-Mullerian Hormone
keywords: folliculogenesis; photostimulation; seasonal reproduction