title: Naive-like conversion overcomes the limited differentiation capacity of induced pluripotent stem cells.
abstract: Although induced pluripotent stem (iPS) cells are indistinguishable from ES cells in their expression of pluripotent markers, their differentiation into targeted cells is often limited. Here, we examined whether the limited capacity of iPS cells to differentiate into neural lineage cells could be mitigated by improving their base-line level of pluripotency, i.e. by converting them into the so-called "naive" state. In this study, we used rabbit iPS and ES cells because of the easy availability of both cell types and their typical primed state characters. Repeated passages of the iPS cells permitted their differentiation into early neural cell types (neural stem cells, neurons, and glial astrocytes) with efficiencies similar to ES cells. However, unlike ES cells, their ability to differentiate later into neural cells (oligodendrocytes) was severely compromised. In contrast, after these iPS cells had been converted to a naive-like state, they readily differentiated into mature oligodendrocytes developing characteristic ramified branches, which could not be attained even with ES cells. These results suggest that the naive-like conversion of iPS cells might endow them with a higher differentiation capacity.
authors.affiliation: From the Organization for Promotion of Tenure Track, University of Miyazaki, 5200, Kibara, Kiyotake, Miyazaki 889-1692,; the RIKEN BioResource Center, Tsukuba, Ibaraki 305-0074,; PRESTO, Japan Science and Technology Agency, 4-1-8 Honcho, Kawaguchi, Saitama 332-0012,. Electronic address: a-honda@med.miyazaki-u.ac.jp.
authors.forename: Arata
authors.initials: A
authors.lastname: Honda
authors.affiliation: the RIKEN BioResource Center, Tsukuba, Ibaraki 305-0074.
authors.forename: Masanori
authors.initials: M
authors.lastname: Hatori
authors.affiliation: the RIKEN BioResource Center, Tsukuba, Ibaraki 305-0074.
authors.forename: Michiko
authors.initials: M
authors.lastname: Hirose
authors.affiliation: From the Organization for Promotion of Tenure Track, University of Miyazaki, 5200, Kibara, Kiyotake, Miyazaki 889-1692.
authors.forename: Chizumi
authors.initials: C
authors.lastname: Honda
authors.affiliation: From the Organization for Promotion of Tenure Track, University of Miyazaki, 5200, Kibara, Kiyotake, Miyazaki 889-1692.
authors.forename: Haruna
authors.initials: H
authors.lastname: Izu
authors.affiliation: the RIKEN BioResource Center, Tsukuba, Ibaraki 305-0074,; the Graduate School of Life and Environmental Science, University of Tsukuba, Tsukuba, Ibaraki 305-8572, and.
authors.forename: Kimiko
authors.initials: K
authors.lastname: Inoue
authors.affiliation: the RIKEN BioResource Center, Tsukuba, Ibaraki 305-0074.
authors.forename: Ryutaro
authors.initials: R
authors.lastname: Hirasawa
authors.affiliation: the RIKEN BioResource Center, Tsukuba, Ibaraki 305-0074.
authors.forename: Shogo
authors.initials: S
authors.lastname: Matoba
authors.affiliation: the RIKEN BioResource Center, Tsukuba, Ibaraki 305-0074.
authors.forename: Sumie
authors.initials: S
authors.lastname: Togayachi
authors.affiliation: the RIKEN BioResource Center, Tsukuba, Ibaraki 305-0074.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Miyoshi
authors.affiliation: the RIKEN BioResource Center, Tsukuba, Ibaraki 305-0074,; the Graduate School of Life and Environmental Science, University of Tsukuba, Tsukuba, Ibaraki 305-8572, and; the Center for Disease Biology and Integrative Medicine, 5 Faculty of Medicine, University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan.
authors.forename: Atsuo
authors.initials: A
authors.lastname: Ogura
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000166:cytology / Q000378:metabolism; D002454:Cell Differentiation; D002478:Cells, Cultured; D053595:Embryonic Stem Cells / Q000166:cytology / Q000378:metabolism; D057026:Induced Pluripotent Stem Cells / Q000166:cytology* / Q000378:metabolism*; D058953:Neural Stem Cells / Q000166:cytology / Q000378:metabolism; D009836:Oligodendroglia / Q000166:cytology / Q000378:metabolism; D011817:Rabbits
keywords: Cell Culture; Embryonic Stem Cell; Induced Pluripotent Stem (iPS) Cell; Naive; Neurodifferentiation; Oligodendrocytes; Primed; Rabbit