title: [Effect of sinusoidal electromagnetic fields on rats femur tissue cultivation in vitro].
abstract: The present research was aimed to investigate the effect of 50Hz, 1. 8mT of sinusoidal electromagnetic fields(SEMFs)on femur tissue cultivation in vitro. The rat femur tissue was isolated from SD rats by method of enzyme digestion, and randomly divided into two groups: SEMFs group and control. The femur tissue of SEMFs groups were exposured under 50Hz 1. 8mT of SEMFs for 1. 5h/time/d, but those in the control groups were without SEMFs treatment. The correlative gene was detected by the Real-time RT-PCR that after SEMFs treatment for 0 (first times treatments is 0 days), 1, 2, 3, 4 and 5d. The alkaline phosphatase (ALP) activity was measured after SEMFs treatment for 3, 6, 9 and 12d respectively. The calcium content was detected after SEMFs treatment for 3, 6, 9 and 12d. The results showed that the OPG and Collagen-1 mRNA expression level was kept at a relatively stable level by SEMFs in the SEMFs group significantly. The Runx-2 mRNA expression level was significantly increased after the SEMFs treatment for 1d and 5d. The ALP activity of femur tissue was significantly increased alter SEMFs treatment for 3d and 9d. The calcium content was higher than untreated groups after SEMFs treatment for 6, 9 and 12d. The SEMFs promoted OPG, Collagen-1, Runx-2 mRNA expression level, ALP activity and calcium content. The result indicated that SEMFs increased the metabolism activity of femur tissues.
authors.affiliation: Institute of Orthopaedics, Lanzhou General Hospital of PLA, Lanzhou 730050, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Zhou
authors.forename: Jiaqi
authors.initials: J
authors.lastname: Wang
authors.forename: Xiaoni
authors.initials: X
authors.lastname: Ma
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Guo
authors.forename: Baofeng
authors.initials: B
authors.lastname: Ge
authors.forename: Keming
authors.initials: K
authors.lastname: Chen
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D002118:Calcium / Q000378:metabolism; D024042:Collagen Type I / Q000235:genetics / Q000378:metabolism; D050659:Core Binding Factor Alpha 1 Subunit / Q000235:genetics / Q000378:metabolism; D004574:Electromagnetic Fields; D005260:Female; D005269:Femur / Q000254:growth & development*; D008297:Male; D053244:Osteoprotegerin / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D046509:Tissue Culture Techniques / Q000379:methods*
chemical_list: D024042:Collagen Type I; D050659:Core Binding Factor Alpha 1 Subunit; D053244:Osteoprotegerin; D012333:RNA, Messenger; C493717:Runx2 protein, rat; C506032:Tnfrsf11b protein, rat; D000469:Alkaline Phosphatase; D002118:Calcium