title: Insights on vitamin D's role in cardiovascular disease: investigating the association of 25-hydroxyvitamin D with the dimethylated arginines.
abstract: Accumulating evidence has stipulated a strong correlation between vitamin D (vitD) deficiency and cardiovascular disease (CVD); however, a mechanistic link is missing. This study investigated the association of vitD with endothelial dysfunction parameters. Subjects comprised male patients with verified coronary artery disease (CAD) (n=69) and age- and sex-matched controls (n=20). 25-Hydroxyvitamin D [25(OH)D] was determined using high performance liquid chromatography with ultraviolet detection whereas asymmetric and symmetric dimethylarginine (ADMA and SDMA, respectively) were determined by liquid chromatography-mass spectrometry. Nitric oxide (NO) was determined spectrophotometrically and high-sensitivity C-reactive protein (hs-CRP) was determined using enzyme-linked immunosorbent assay (ELISA). Comparison of mean 25(OH)D concentrations of patients and controls yielded a significant result (p=0.0002). 25(OH)D2 was dominant in patients whereas 25(OH)D3 was dominant in controls (p=0.003 and 0.001, respectively). Comparison of mean ADMA and SDMA concentrations of patients exhibiting normal and suboptimal vitD yielded insignificant results (p=0.692 and 0.998, respectively). Significant results were obtained from the comparison of mean hs-CRP and NO concentrations of patients exhibiting normal and suboptimal vitD (p=0.035 and 0.031, respectively). Results suggest involvement of vitD with the NO system, however not via modulation of the dimethylated arginines. A potential anti-inflammatory activity for vitD is also raised.
authors.affiliation: Biochemistry Department, Faculty of Pharmacy and Biotechnology, German University in Cairo. abu.el.maaty@gmail.com
authors.forename: Mohamed A
authors.initials: MA
authors.lastname: Abu El Maaty
authors.forename: Sally I
authors.initials: SI
authors.lastname: Hassanein
authors.forename: Rasha S
authors.initials: RS
authors.lastname: Hanafi
authors.forename: Mohamed Z
authors.initials: MZ
authors.lastname: Gad
mesh_terms: D015652:25-Hydroxyvitamin D 2 / Q000097:blood; D000328:Adult; D001120:Arginine / Q000031:analogs & derivatives* / Q000097:blood; D002097:C-Reactive Protein / Q000378:metabolism*; D016022:Case-Control Studies; D003324:Coronary Artery Disease / Q000097:blood / Q000209:etiology*; D004730:Endothelium, Vascular; D006801:Humans; D008297:Male; D008875:Middle Aged; D009569:Nitric Oxide / Q000378:metabolism*; D014807:Vitamin D / Q000031:analogs & derivatives / Q000097:blood*; D014808:Vitamin D Deficiency / Q000097:blood / Q000150:complications*
chemical_list: C487735:dimethylarginine; D014807:Vitamin D; D015652:25-Hydroxyvitamin D 2; D009569:Nitric Oxide; D002097:C-Reactive Protein; D001120:Arginine; C104450:25-hydroxyvitamin D