title: Transmission of chimeric HIV by mating in conventional mice: prevention by pre-exposure antiretroviral therapy and reduced susceptibility during estrus.
abstract: Heterosexual transmission accounts for the majority of new human immunodeficiency virus (HIV) cases worldwide. The current approach to investigate HIV heterosexual transmission in animals involves application of virus stock to the vaginal surface, a method that does not reproduce the physiological conditions of vaginal intercourse that influence the rate of transmission. We have previously described efficient infection of conventional mice using EcoHIV/NL4-3 and EcoHIV/NDK, chimeric HIV molecular clones constructed to express all HIV structural and regulatory genes except envelope, which is replaced by a rodent-tropic envelope gene. Here we investigated whether EcoHIV/NDK-infected male mice transmit virus to females during coitus, and the sensitivity of this transmission to HIV pre-exposure prophylaxis and the estrus state. Our general approach was to allow mating between EcoHIV/NDK-infected male mice and uninfected females for 1-7 nights. At 1-6 weeks after mating, mice were euthanized and virus burdens were measured by quantitative PCR (qPCR) amplification of HIV RNA or DNA in peritoneal macrophages, inguinal lymph node cells, spleen cells or vas deferens, or by ELISA for antibodies to HIV Gag. We found that 70-100% of female mice mated to EcoHIV/NDK-infected males acquired infection. Pericoital treatment of females with either 2',3'-dideoxcytidine (ddC) or tenofovir largely prevented their EcoHIV/NDK infection by mating (P<0.05 and P<0.003, respectively). In males, T cells were dispensable for virus transmission. The rate of EcoHIV/NDK sexual transmission to females in estrus declined sharply (P=0.003) but their infection by injection was unaffected, indicating that the local environment in the female reproductive tract influences susceptibility to HIV. We conclude that this system of EcoHIV/NDK transmission during mouse mating reproduces key features of heterosexual transmission of HIV in humans and can be used to investigate its biology and control.
authors.affiliation: Molecular Virology Division, St Luke's-Roosevelt Hospital Center, Columbia University Medical Center, New York, NY 10019, USA.
authors.forename: Eran
authors.initials: E
authors.lastname: Hadas
authors.forename: Wei
authors.initials: W
authors.lastname: Chao
authors.forename: Hongxia
authors.initials: H
authors.lastname: He
authors.forename: Manisha
authors.initials: M
authors.lastname: Saini
authors.forename: Eleen
authors.initials: E
authors.lastname: Daley
authors.forename: Mohammed
authors.initials: M
authors.lastname: Saifuddin
authors.forename: Galina
authors.initials: G
authors.lastname: Bentsman
authors.forename: Eric
authors.initials: E
authors.lastname: Ganz
authors.forename: David J
authors.initials: DJ
authors.lastname: Volsky
authors.forename: Mary Jane
authors.initials: MJ
authors.lastname: Potash
mesh_terms: D000818:Animals; D044966:Anti-Retroviral Agents / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use*; D002678:Chimera; D003307:Copulation; D004198:Disease Susceptibility; D004971:Estrus / Q000187:drug effects / Q000502:physiology*; D005260:Female; D006678:HIV / Q000502:physiology*; D015658:HIV Infections / Q000188:drug therapy / Q000517:prevention & control* / Q000635:transmission*; D006801:Humans; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D023421:Models, Animal; D013154:Spleen / Q000187:drug effects / Q000821:virology; D013601:T-Lymphocytes / Q000821:virology; D014621:Vagina / Q000187:drug effects / Q000502:physiology / Q000821:virology; D014649:Vas Deferens / Q000187:drug effects / Q000821:virology; D019562:Viral Load / Q000187:drug effects
chemical_list: D044966:Anti-Retroviral Agents