title: [ZNF217 expression correlates with the biological behavior of human ovarian cancer cells].
abstract: OBJECTIVE
  To investigate the correlation of ZNF217 gene expression with the biological behavior of human ovarian cancer HO-8910 cells.
  
  
  METHODS
  The expression of ZNF217 in ovarian carcinoma cell lines was detected by RT-PCR and Western blot, respectively. The biological behaviors of the transfectants were investigated by MTT, in vitro Boyden chamber and in vivo invasion assay, respectively.
  
  
  RESULTS
  RT-PCR and Western blotting revealed that transfection of ZNF217 into the HO-8910 cells significantly increased their proliferation along with markedly enhanced in vitro and in vivo invasion and metastatic abilities. MTT assay showed that the proliferation ability of pEGFP-N1-ZNF217/HO-8910 cells was significantly higher than that of pEGFP-N1/HO-8910 cells and HO-8910 cells (P < 0.001). The Boyden chamber assay showed that the numbers of migrating pEGFP-N1-ZNF217/HO-8910, pEGFP-N1/HO-8910 and HO-8910 cells were (141.25 ± 13.91) cells/200×field, (82.50 ± 11.73) cells/200×field and (81.75 ± 12.12)cells/200×field, with a significant difference between them (F = 29.247, P < 0.001). The nude mouse experiment showed that the in vivo tumor formation ability of pEGFP-N1-ZNF217/HO-8910 cells was significantly higher than that of pEGFP-N1/HO-8910 cells (P < 0.001).
  
  
  CONCLUSIONS
  ZNF217 gene plays an important role in the invasion and metastasis of ovarian cancer. ZNF217 gene expression may be a useful marker indicating invasion and metastasis of ovarian cancer.
authors.affiliation: Department of Gynecology and Obstetrics, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.forename: Lan-lin
authors.initials: LL
authors.lastname: Song
authors.forename: Mei
authors.initials: M
authors.lastname: Zhong
mesh_terms: D000818:Animals; D014408:Biomarkers, Tumor / Q000378:metabolism; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D018284:Cystadenocarcinoma, Serous / Q000378:metabolism / Q000473:pathology; D005260:Female; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D009368:Neoplasm Transplantation; D010051:Ovarian Neoplasms / Q000378:metabolism / Q000473:pathology; D010957:Plasmids; D012333:RNA, Messenger / Q000378:metabolism; D011897:Random Allocation; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism; D014162:Transfection; D047368:Tumor Burden
chemical_list: D014408:Biomarkers, Tumor; D012333:RNA, Messenger; D015534:Trans-Activators; C113698:ZNF217 protein, human