title: Effects of green tea and vitamin E in the testicular tissue of streptozotocin-induced diabetic rats.
abstract: OBJECTIVE
  To investigate the possible therapeutic or protective effects of green tea in diabetic rat's testicular tissue, either as a single agent, or together with vitamin E.
  
  
  METHODS
  The present study was carried out at the Faculty of Medicine, Gazi University, Ankara, Turkey from May to August 2011 for 10 weeks. Forty-eight adult male Wistar albino rats, weighting 250-300 g, were divided into 8 groups: control; nondiabetic vitamin E (0.4 mg/kg/NG); nondiabetic green tea (300 mg/kg/NG); nondiabetic vitamin E plus green tea administered groups; diabetic group (60 mg/kg/IV streptozotocin); diabetic vitamin E; diabetic green tea; and diabetic vitamin E plus green tea administered groups. Proliferative and apoptotic indexes were determined using anti-PCNA antibody immunohistochemistry and TUNEL assays respectively. Tubule degeneration was evaluated using the Johnson's score and also seminiferous tubules diameters, epithelial thickness were measured.
  
  
  RESULTS
  Histopathological examination in diabetic group revealed degenerative changes in the seminiferous tubules together with a statistically significant decrease in PCNA positive cells, in epithelial thickness, diameter of the tubules and in Johnson's score, while exhibited an increase in the number of apoptotic cells. When all these findings are considered together, the most successful protective effects in diabetes were obtained in the combined antioxidant group.
  
  
  CONCLUSIONS
  Combined therapy of vitamin E and green tea in diabetes was more effective than monotherapy. Therefore, these antioxidants may be use as a supporting therapy for reproductive dysfunction.
authors.affiliation: Department of Histology and Embryology, Faculty of Medicine, Gazi University, Besevler 06500, Ankara, Turkey. gtake@gazi.edu.tr
authors.forename: Gulnur T
authors.initials: GT
authors.lastname: Kaplanoglu
authors.forename: Meltem
authors.initials: M
authors.lastname: Bahcelioglu
authors.forename: Rabet
authors.initials: R
authors.lastname: Gozil
authors.forename: Fatma
authors.initials: F
authors.lastname: Helvacioglu
authors.forename: Ece
authors.initials: E
authors.lastname: Buru
authors.forename: Mustafa A
authors.initials: MA
authors.lastname: Tekindal
authors.forename: Deniz
authors.initials: D
authors.lastname: Erdogan
authors.forename: Engin
authors.initials: E
authors.lastname: Calguner
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology / Q000627:therapeutic use*; D017209:Apoptosis / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D003921:Diabetes Mellitus, Experimental / Q000139:chemically induced / Q000188:drug therapy*; D008297:Male; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use*; D051381:Rats; D017208:Rats, Wistar; D012671:Seminiferous Tubules / Q000473:pathology*; D013311:Streptozocin; D013662:Tea; D013737:Testis / Q000473:pathology*; D014810:Vitamin E / Q000494:pharmacology / Q000627:therapeutic use*
chemical_list: D000975:Antioxidants; D010936:Plant Extracts; D013662:Tea; D014810:Vitamin E; D013311:Streptozocin