title: Aurora A kinase and its substrate TACC3 are required for central spindle assembly.
abstract: Cell division entails a marked reorganization of the microtubule network to form the spindle, a molecular machine that ensures accurate chromosome segregation to the daughter cells. Spindle organization is highly dynamic throughout mitosis and requires the activity of several kinases and complex regulatory mechanisms. Aurora A (AurA) kinase is essential for the assembly of the metaphase bipolar spindle and, thus, it has been difficult to address its function during the last phases of mitosis. Here, we examine the consequences of inhibiting AurA in cells undergoing anaphase, and show that AurA kinase activity is necessary for the assembly of a robust central spindle during anaphase. We also identify TACC3 as an AurA substrate essential in central spindle formation.
authors.affiliation: Centre for Genomic Regulation (CRG).
authors.forename: Antonios
authors.initials: A
authors.lastname: Lioutas
authors.forename: Isabelle
authors.initials: I
authors.lastname: Vernos
mesh_terms: D064096:Aurora Kinase A / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D008869:Microtubule-Associated Proteins / Q000235:genetics / Q000378:metabolism*; D047428:Protein Kinase Inhibitors / Q000494:pharmacology; D008941:Spindle Apparatus / Q000187:drug effects / Q000378:metabolism*
chemical_list: D008869:Microtubule-Associated Proteins; D047428:Protein Kinase Inhibitors; C120551:TACC3 protein, human; C576917:AURKA protein, human; D064096:Aurora Kinase A