title: Integrative genomic analysis of the human immune response to influenza vaccination.
abstract: Identification of the host genetic factors that contribute to variation in vaccine responsiveness may uncover important mechanisms affecting vaccine efficacy. We carried out an integrative, longitudinal study combining genetic, transcriptional, and immunologic data in humans given seasonal influenza vaccine. We identified 20 genes exhibiting a transcriptional response to vaccination, significant genotype effects on gene expression, and correlation between the transcriptional and antibody responses. The results show that variation at the level of genes involved in membrane trafficking and antigen processing significantly influences the human response to influenza vaccination. More broadly, we demonstrate that an integrative study design is an efficient alternative to existing methods for the identification of genes involved in complex traits. DOI:http://dx.doi.org/10.7554/eLife.00299.001.
authors.affiliation: Department of Molecular and Human Genetics , Baylor College of Medicine , Houston , United States ; Department of Medicine , Baylor College of Medicine , Houston , United States.
authors.forename: Luis M
authors.initials: LM
authors.lastname: Franco
authors.forename: Kristine L
authors.initials: KL
authors.lastname: Bucasas
authors.forename: Janet M
authors.initials: JM
authors.lastname: Wells
authors.forename: Diane
authors.initials: D
authors.lastname: Niño
authors.forename: Xueqing
authors.initials: X
authors.lastname: Wang
authors.forename: Gladys E
authors.initials: GE
authors.lastname: Zapata
authors.forename: Nancy
authors.initials: N
authors.lastname: Arden
authors.forename: Alexander
authors.initials: A
authors.lastname: Renwick
authors.forename: Peng
authors.initials: P
authors.lastname: Yu
authors.forename: John M
authors.initials: JM
authors.lastname: Quarles
authors.forename: Molly S
authors.initials: MS
authors.lastname: Bray
authors.forename: Robert B
authors.initials: RB
authors.lastname: Couch
authors.forename: John W
authors.initials: JW
authors.lastname: Belmont
authors.forename: Chad A
authors.initials: CA
authors.lastname: Shaw
mesh_terms: D000293:Adolescent; D000328:Adult; D000914:Antibodies, Viral / Q000097:blood; D015415:Biomarkers / Q000097:blood; D005260:Female; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D005819:Genetic Markers; D023281:Genomics / Q000379:methods; D005838:Genotype; D054884:Host-Pathogen Interactions; D006801:Humans; D056724:Immunity, Humoral / Q000235:genetics*; D053118:Influenza A Virus, H1N1 Subtype / Q000276:immunology*; D053122:Influenza A Virus, H3N2 Subtype / Q000276:immunology*; D009981:Influenza B virus / Q000276:immunology*; D007252:Influenza Vaccines / Q000008:administration & dosage / Q000276:immunology*; D007251:Influenza, Human / Q000235:genetics* / Q000276:immunology / Q000517:prevention & control* / Q000821:virology; D008137:Longitudinal Studies; D008297:Male; D010597:Pharmacogenetics; D010641:Phenotype; D020641:Polymorphism, Single Nucleotide; D013997:Time Factors; D014158:Transcription, Genetic; D014611:Vaccination; D055815:Young Adult
chemical_list: D000914:Antibodies, Viral; D015415:Biomarkers; D005819:Genetic Markers; D007252:Influenza Vaccines
keywords: Complex-trait genetics; Human; Human genetics; Integrative biology; Systems biology; Vaccines; eQTL