title: Selective encapsulation of hemoproteins from mammalian cells using mesoporous metal oxide nanoparticles.
abstract: A key requirement in successful protein encapsulation is the fabrication of selective protein supercaptors that are not impeded by the physical shape and three-dimensional hydrodynamics of the protein, exhibit minimal clogging effect but with high protein retention, and with uniformly sized adsorbent pores. We report a novel nanomagnet-selective supercaptor approach in the encapsulation of hemoprotein from mammalian cells using mesoporous metal oxide nanoparticles (NPs). Different morphologies of mesoporous NiO and Fe3O4 NPs were fabricated. Among these nanoadsorbents, NiO nanoroses (NRs) had higher loading capacity of hemoprotein than NiO nanospheres (NSs) and nanoplatelets (NPLs), or even superparamagnetic Fe3O4 NPs. The key finding of this study was that mesoporous NiO nanomagnet supercaptors show exceptional encapsulation and selective separation of high-concentration Hb from human blood. In this induced-fit separation model, in addition to the heme group distributions and protein-carrier binding energy, the morphology and magnetic properties of NiO NPs had a key function in broadening the controlled immobilization affinity and selectivity of hemoproteins. In addition, thermodynamics, kinetics, and theoretical studies were carried out to investigate the optimal performance of protein adsorption.
authors.affiliation: National Institute for Materials Science (NIMS), 1-2-1 Sengen, Tsukuba-shi, Ibaraki-ken 305-0047, Japan; Graduate School for Advanced Science and Engineering, Waseda University, 3-4-1 Okubo, Shinjuku-ku, Tokyo 169-8555, Japan.
authors.forename: Mohamed
authors.initials: M
authors.lastname: Khairy
authors.affiliation: National Institute for Materials Science (NIMS), 1-2-1 Sengen, Tsukuba-shi, Ibaraki-ken 305-0047, Japan; Graduate School for Advanced Science and Engineering, Waseda University, 3-4-1 Okubo, Shinjuku-ku, Tokyo 169-8555, Japan. Electronic address: sherif.elsafty@nims.go.jp.
authors.forename: Sherif A
authors.initials: SA
authors.lastname: El-Safty
mesh_terms: D000327:Adsorption; D000818:Animals; D005290:Ferric Compounds / Q000737:chemistry*; D006420:Hemeproteins / Q000378:metabolism*; D006801:Humans; D055767:Immobilized Proteins / Q000378:metabolism*; D008322:Mammals / Q000378:metabolism*; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D008962:Models, Theoretical; D054118:Nanospheres; D009532:Nickel / Q000737:chemistry*; D016062:Porosity; D053838:Scattering, Small Angle; D013056:Spectrophotometry, Ultraviolet; D013997:Time Factors; D014961:X-Ray Diffraction
chemical_list: D005290:Ferric Compounds; D006420:Hemeproteins; D055767:Immobilized Proteins; C000499:ferric oxide; D009532:Nickel; C028007:nickel monoxide
keywords: Hemoproteins; Mammalian cells; Mesoporous; Metal oxides; Selective encapsulation