title: An additional substrate binding site in a bacterial phenylalanine hydroxylase.
abstract: Phenylalanine hydroxylase (PAH) is a non-heme iron enzyme that catalyzes oxidation of phenylalanine to tyrosine, a reaction that must be kept under tight regulatory control. Mammalian PAH has a regulatory domain in which binding of the substrate leads to allosteric activation of the enzyme. However, the existence of PAH regulation in evolutionarily distant organisms, for example some bacteria in which it occurs, has so far been underappreciated. In an attempt to crystallographically characterize substrate binding by PAH from Chromobacterium violaceum, a single-domain monomeric enzyme, electron density for phenylalanine was observed at a distal site 15.7 Å from the active site. Isothermal titration calorimetry (ITC) experiments revealed a dissociation constant of 24 ± 1.1 μM for phenylalanine. Under the same conditions, ITC revealed no detectable binding for alanine, tyrosine, or isoleucine, indicating the distal site may be selective for phenylalanine. Point mutations of amino acid residues in the distal site that contact phenylalanine (F258A, Y155A, T254A) led to impaired binding, consistent with the presence of distal site binding in solution. Although kinetic analysis revealed that the distal site mutants suffer discernible loss of their catalytic activity, X-ray crystallographic analysis of Y155A and F258A, the two mutants with the most noticeable decrease in activity, revealed no discernible change in the structure of their active sites, suggesting that the effect of distal binding may result from protein dynamics in solution.
authors.affiliation: Brown Laboratory of Chemistry, Department of Chemistry, Purdue University, 560 Oval Drive, West Lafayette, IN 47907, USA.
authors.forename: Judith A
authors.initials: JA
authors.lastname: Ronau
authors.forename: Lake N
authors.initials: LN
authors.lastname: Paul
authors.forename: Julian E
authors.initials: JE
authors.lastname: Fuchs
authors.forename: Isaac R
authors.initials: IR
authors.lastname: Corn
authors.forename: Kyle T
authors.initials: KT
authors.lastname: Wagner
authors.forename: Klaus R
authors.initials: KR
authors.lastname: Liedl
authors.forename: Mahdi M
authors.initials: MM
authors.lastname: Abu-Omar
authors.forename: Chittaranjan
authors.initials: C
authors.lastname: Das
mesh_terms: D055162:Biocatalysis; D020134:Catalytic Domain; D002861:Chromobacterium / Q000201:enzymology*; D018360:Crystallography, X-Ray; D007700:Kinetics; D008958:Models, Molecular; D009154:Mutation; D010649:Phenylalanine / Q000378:metabolism; D010651:Phenylalanine Hydroxylase / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding
chemical_list: D010649:Phenylalanine; D010651:Phenylalanine Hydroxylase