title: Evidence that adrenergic and cholinergic but not capsaicin-sensitive nerves are involved in the nerve-mediated contraction of the guinea pig seminal vesicle.
abstract: OBJECTIVE
  The neurotransmitters participating in the nerve-mediated contraction of the guinea pig seminal vesicle (GPSV) have not been firmly established. There is debate as to the mediating role of norepinephrine and acetylcholine.
  
  
  METHODS
  We have used longitudinally and circularly oriented strips of GPSV and activated their intramural nerves by electrical field stimulation (5 and 10 Hz for 30 s).
  
  
  RESULTS
  Contractile responses to stimulation were enhanced by a cholinesterase inhibitor and reduced by the adrenergic α-receptor antagonist phentolamine (2.5 μmol/l). Atropine (1 μmol/l) significantly reduced responses in longitudinal preparations; a less consistent inhibition was found in circular preparations. The sensory neuron stimulant and blocker capsaicin was without effect.
  
  
  CONCLUSIONS
  It is concluded that adrenergic nerves and also acetylcholine mediate the contractile response of the GPSV.
authors.affiliation: Department of Pharmacology, University Medical School of Pécs, Pécs, Hungary.
authors.forename: Rita
authors.initials: R
authors.lastname: Benko
authors.forename: Veronika
authors.initials: V
authors.lastname: Szombati
authors.forename: Lorand
authors.initials: L
authors.lastname: Bartho
mesh_terms: D000317:Adrenergic alpha-Antagonists / Q000494:pharmacology; D000818:Animals; D001285:Atropine / Q000494:pharmacology; D002211:Capsaicin / Q000494:pharmacology; D002800:Cholinesterase Inhibitors / Q000494:pharmacology; D004558:Electric Stimulation; D006168:Guinea Pigs; D066298:In Vitro Techniques; D008297:Male; D018727:Muscarinic Antagonists / Q000494:pharmacology; D009119:Muscle Contraction / Q000187:drug effects / Q000502:physiology*; D010646:Phentolamine / Q000494:pharmacology; D010830:Physostigmine / Q000494:pharmacology; D012669:Seminal Vesicles / Q000187:drug effects / Q000294:innervation* / Q000502:physiology*; D018689:Sensory System Agents / Q000494:pharmacology
chemical_list: D000317:Adrenergic alpha-Antagonists; D002800:Cholinesterase Inhibitors; D018727:Muscarinic Antagonists; D018689:Sensory System Agents; D001285:Atropine; D010830:Physostigmine; D002211:Capsaicin; D010646:Phentolamine