title: Correlation between ¹⁸F-FDG uptake and the expression of glucose transporter-1 and hypoxia-inducible factor-1α in transplanted VX2 tumors.
abstract: OBJECTIVE
  We investigated the correlation between fluorine-18-fluorodeoxyglucose (F-FDG) uptake and glucose transporter-1 (GLUT-1) and hypoxia-inducible factor-1α (HIF-1α) expression in a rabbit lung VX2 tumor model.
  
  
  METHODS
  Twenty-four VX2 tumor-bearing rabbits underwent F-FDG PET/computed tomography (CT) at 4, 5, 6, and 7 weeks after implantation. PET/CT images were obtained to monitor the tumor/normal tissue (T/N) ratio in each period. Six rabbits were randomly killed after PET/CT, and immunohistochemical staining was used to assess GLUT-1 and HIF-1α expression. We investigated the correlations of F-FDG uptake with GLUT-1 and HIF-1α expression and the pathological tumor (p-tumor) size as well as those of the lymph node metastasis (p-N) stage with the T/N ratio, p-tumor size, and GLUT-1 and HIF-1α expression.
  
  
  RESULTS
  The T/N ratio increased gradually over time before subsequently decreasing. The T/N ratio was significantly influenced by time (F=54.63, P<0.05) and was significantly correlated with GLUT-1 (r=0.53, P<0.01) and HIF-1α (r=0.44, P<0.01) expression and p-tumor size (r=0.58, P<0.05). A significant positive correlation between GLUT-1 expression and HIF-1α expression was also identified (r=0.58, P<0.05). The p-N stage showed a significant association with the T/N ratio (P<0.05) but not with GLUT-1/HIF-1α expression (P>0.05).
  
  
  CONCLUSIONS
  F-FDG uptake is closely correlated with GLUT-1 and HIF-1α expression, tumor size, and lymph node metastasis.
authors.affiliation: Department of aNuclear Medicine, The First Affiliated Hospital of Anhui Medical University, China.
authors.forename: Huiqin
authors.initials: H
authors.lastname: Xu
authors.forename: Biao
authors.initials: B
authors.lastname: Li
authors.forename: Wenjing
authors.initials: W
authors.lastname: Yu
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
authors.forename: Xuefeng
authors.initials: X
authors.lastname: Zhao
authors.forename: Yuanchun
authors.initials: Y
authors.lastname: Yao
authors.forename: Dake
authors.initials: D
authors.lastname: Huang
mesh_terms: D000818:Animals; D001692:Biological Transport; D045744:Cell Line, Tumor; D002471:Cell Transformation, Neoplastic; D019788:Fluorodeoxyglucose F18 / Q000378:metabolism*; D015972:Gene Expression Regulation, Neoplastic; D051272:Glucose Transporter Type 1 / Q000378:metabolism*; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000378:metabolism*; D008175:Lung Neoplasms / Q000000981:diagnostic imaging / Q000378:metabolism* / Q000473:pathology*; D009362:Neoplasm Metastasis; D009367:Neoplasm Staging; D049268:Positron-Emission Tomography; D011817:Rabbits; D014057:Tomography, X-Ray Computed; D047368:Tumor Burden
chemical_list: D051272:Glucose Transporter Type 1; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D019788:Fluorodeoxyglucose F18