title: The vesicular monoamine transporter (VMAT-2) inhibitor tetrabenazine induces tremulous jaw movements in rodents: implications for pharmacological models of parkinsonian tremor.
abstract: Tetrabenazine (TBZ) is a reversible inhibitor of vesicular monoamine storage that is used to treat Huntington's disease. TBZ preferentially depletes striatal dopamine (DA), and patients being treated with TBZ often experience parkinsonian side effects. The present studies were conducted to investigate the ability of TBZ to induce tremulous jaw movements (TJMs), which are a rodent model of parkinsonian tremor, and to determine if interference with adenosine A2A receptor transmission can attenuate TJMs and other motor effects of TBZ. In rats, TBZ (0.25-2.0mg/kg) significantly induced TJMs, which primarily occurred in the 3.0-7.5-Hz frequency range. The adenosine A2A antagonist MSX-3 (1.25-10.0mg/kg) significantly attenuated the TJMs induced by 2.0mg/kg TBZ in rats, and also significantly reduced the display of catalepsy and locomotor suppression induced by TBZ. In mice, TBZ (2.5-10.0mg/kg) dose dependently induced TJMs, and adenosine A2A receptor knockout mice showed significantly fewer TJMs compared to wild-type controls. MSX-3 (2.5-10.0mg/kg) also significantly reduced TBZ-induced TJMs in CD1 mice. To provide a cellular marker of these pharmacological conditions, we examined c-Fos expression in the ventrolateral neostriatum (VLS). TBZ (2.0mg/kg) significantly increased the number of c-Fos-positive cells in the VLS, which is indicative of reduced DA D2 receptor transmission, and 10.0mg/kg MSX-3 significantly attenuated the TBZ-induced c-Fos expression. These results indicate that TBZ induces tremor as measured by the TJM model, and that pharmacological antagonism and genetic deletion of adenosine A2A receptors are capable of attenuating this oral tremor.
authors.affiliation: Department of Psychology, University of Connecticut, Storrs, CT 06269-1020, USA.
authors.forename: S J
authors.initials: SJ
authors.lastname: Podurgiel
authors.forename: E J
authors.initials: EJ
authors.lastname: Nunes
authors.forename: S E
authors.initials: SE
authors.lastname: Yohn
authors.forename: J
authors.initials: J
authors.lastname: Barber
authors.forename: A
authors.initials: A
authors.lastname: Thompson
authors.forename: M
authors.initials: M
authors.lastname: Milligan
authors.forename: C A
authors.initials: CA
authors.lastname: Lee
authors.forename: L
authors.initials: L
authors.lastname: López-Cruz
authors.forename: M
authors.initials: M
authors.lastname: Pardo
authors.forename: O
authors.initials: O
authors.lastname: Valverde
authors.forename: C
authors.initials: C
authors.lastname: Lendent
authors.forename: Y
authors.initials: Y
authors.lastname: Baqi
authors.forename: C E
authors.initials: CE
authors.lastname: Müller
authors.forename: M
authors.initials: M
authors.lastname: Correa
authors.forename: J D
authors.initials: JD
authors.lastname: Salamone
mesh_terms: D018759:Adrenergic Uptake Inhibitors / Q000494:pharmacology*; D000818:Animals; D002375:Catalepsy / Q000139:chemically induced / Q000523:psychology; D003627:Data Interpretation, Statistical; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D007568:Jaw / Q000502:physiology*; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D009043:Motor Activity / Q000187:drug effects; D009068:Movement / Q000187:drug effects*; D020734:Parkinsonian Disorders / Q000139:chemically induced* / Q000503:physiopathology; D016760:Proto-Oncogene Proteins c-fos / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D043705:Receptor, Adenosine A2A / Q000235:genetics; D013747:Tetrabenazine / Q000494:pharmacology*; D014202:Tremor / Q000139:chemically induced* / Q000503:physiopathology; D050493:Vesicular Monoamine Transport Proteins / Q000037:antagonists & inhibitors*; D014970:Xanthines / Q000494:pharmacology
chemical_list: D018759:Adrenergic Uptake Inhibitors; C113602:MSX 3 compound; D016760:Proto-Oncogene Proteins c-fos; D043705:Receptor, Adenosine A2A; C493454:Slc18a2 protein, rat; D050493:Vesicular Monoamine Transport Proteins; D014970:Xanthines; D013747:Tetrabenazine
keywords: ANOVA; DA; DAB; DMSO; EMG; HD; Huntington’s; Huntington’s disease; KO; NE; PBS; Parkinson’s; TBZ; TJM; VLS; VMAT-2; adenosine; analysis of variance; c-Fos; diaminobenzidine; dimethylsulfoxide; dopamine; electromyographic; knockout; norepinephrine; phosphate buffered saline; tetrabenazine; tremor; tremulous jaw movement; type-2 vesicular monoamine transporter; ventrolateral neostriatum