title: Changes of several acute phase factors in broiler chickens in response to infectious bronchitis virus infection.
abstract: This study was performed to clarify the acute phase response following infectious bronchitis virus inoculation. Ninety clinically healthy 1-d-old Ross chicks were randomly assigned into 2 groups: control (n = 20) and infected group (n = 70). At the age of 20 d, all birds in the infected group were challenged intranasally with allantoic fluid containing 10(5) embryo lethal dose (ELD50)/0.1 mL of the infectious bronchitis virus. Blood samples were collected from 20 clinically healthy and 70 infected chicks at prior and 1, 2, 3, 5, 7, 11, 13, 15, and 20 d postinoculation. On d 1, 7, and 11 postinoculation 4 chickens from the experimental group and 2 chickens from the control group were randomly selected. Their trachea, lungs, and cecal tonsil were collected for virus detection and quantitation by real-time reverse-transcription PCR assay. In the serum the acute phase proteins (haptoglobin and serum amyloid A), pro-inflammatory cytokines (interferon-γ and tumor necrosis factor-α), and serum sialic acid (total, TSA; lipid-bound, LBSA; and protein-bound, PBSA) concentrations were measured using validated standard procedures. All variables were significantly higher in the infected birds after virus inoculation compared with the healthy group (P < 0.05). There were positive correlations between all variables in the infected group. Correlation coefficients were significantly positive between haptoglobin and interferon-γ, LBSA and TSA, and TSA and LBSA (P < 0.05). There were positive correlations among viral RNA and all studied variables; however, these correlations were not statistically significant (P > 0.05).
authors.affiliation: Department of Clinical Studies, Shiraz University, Shiraz, Iran.
authors.forename: Keramat
authors.initials: K
authors.lastname: Asasi
authors.forename: Ali
authors.initials: A
authors.lastname: Mohammadi
authors.forename: Zahra
authors.initials: Z
authors.lastname: Boroomand
authors.forename: Seyedeh Alemeh
authors.initials: SA
authors.lastname: Hosseinian
authors.forename: Saeed
authors.initials: S
authors.lastname: Nazifi
mesh_terms: D000209:Acute-Phase Proteins / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D002645:Chickens; D018352:Coronavirus Infections / Q000378:metabolism / Q000662:veterinary* / Q000821:virology; D001351:Infectious bronchitis virus; D011201:Poultry Diseases / Q000378:metabolism* / Q000821:virology
chemical_list: D000209:Acute-Phase Proteins