title: Isolation and characteristics of matrix vesicles.
abstract: Mineralizing matrix vesicles (MVs) are extracellular organelles produced by chondrocytes, osteoblasts, and odontoblasts under physiological conditions and by vascular smooth muscle cells under pathological conditions. MVs are involved in the early stage of mineralization allowing calcium and phosphate to accumulate, and therefore providing an optimal environment facilitating hydroxyapatite formation. Here, we describe the isolation of MVs from osteoblasts and chondrocytes and present their main characteristics.
authors.affiliation: Equipe Organisation et Dynamique des Membranes Biologiques, UMR-CNRS 5246, Institut de Chimie et Biochimie Moléculaires et Supramoléculaires, Université Claude Bernard-Lyon 1, Université de Lyon, Villeurbanne, France.
authors.forename: René
authors.initials: R
authors.lastname: Buchet
authors.forename: Slawomir
authors.initials: S
authors.lastname: Pikula
authors.forename: David
authors.initials: D
authors.lastname: Magne
authors.forename: Saïda
authors.initials: S
authors.lastname: Mebarek
mesh_terms: D000818:Animals; D001857:Bone Matrix; D002458:Cell Fractionation / Q000379:methods*; D045744:Cell Line, Tumor; D002642:Chick Embryo; D019902:Chondrocytes / Q000737:chemistry*; D022162:Cytoplasmic Vesicles / Q000737:chemistry*; D017886:Durapatite / Q000378:metabolism; D006801:Humans; D009131:Muscle, Smooth, Vascular / Q000737:chemistry; D009804:Odontoblasts / Q000737:chemistry*; D010006:Osteoblasts / Q000737:chemistry*
chemical_list: D017886:Durapatite