title: Aptamer-based label-free detection of PDGF using ruthenium(II) complex as luminescent probe.
abstract: We report a simple, cost-effective, and label-free detection method, consisting of a platelet-derived growth factor (PDGF) binding aptamer and hydrophobic Ru(II) complex as a sensor system for PDGF. The binding of PDGF with the aptamer results in the weakening of the aptamer-Ru(II) complex, monitored by luminescence signal. A substantial enhancement in the luminescence intensity of Ru(II) complex is observed in the presence of aptamer due to the hydrophobic interaction. Upon addition of PDGF, the luminescence intensity is decreased, due to the stronger interaction between the aptamer and PDGF resulting in the displacement of Ru(II) complex to the aqueous solution. Our assay can detect a target specifically in a complex medium such as the mixture of proteins, at a concentration of 0.8 pM.
authors.affiliation: Department of Physical Chemistry, School of Chemistry, Madurai Kamaraj University, Madurai, 625 021, India.
authors.forename: E
authors.initials: E
authors.lastname: Babu
authors.forename: S
authors.initials: S
authors.lastname: Singaravadivel
authors.forename: P
authors.initials: P
authors.lastname: Manojkumar
authors.forename: S
authors.initials: S
authors.lastname: Krishnasamy
authors.forename: G
authors.initials: G
authors.lastname: Gnana kumar
authors.forename: S
authors.initials: S
authors.lastname: Rajagopal
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry*; D008163:Luminescent Measurements / Q000379:methods*; D015336:Molecular Probe Techniques; D015335:Molecular Probes / Q000032:analysis / Q000737:chemistry; D010982:Platelet-Derived Growth Factor / Q000032:analysis* / Q000737:chemistry; D025941:Protein Interaction Mapping / Q000379:methods*; D012428:Ruthenium / Q000032:analysis* / Q000737:chemistry*; D013194:Staining and Labeling
chemical_list: D052157:Aptamers, Nucleotide; D015335:Molecular Probes; D010982:Platelet-Derived Growth Factor; D012428:Ruthenium