title: Comprehensive assay of flavanones in citrus juices and beverages by UHPLC-ESI-MS/MS and derivatization chemistry.
abstract: Flavanones, a class of flavonoids present in large amounts in fruits and vegetables, have been assayed by LC-MS/MS and derivatization chemistry using d0/d3-labelled derivatized internal standards obtained by simple reaction procedures which involves d0/d3 methoxyamine. The assay method considers 13 flavanones including aglycones, neohesperidosides, rutinosides and 3-hydroxy-3-methyl glutaryl derivatives. The strengths of the method consist in a relative short analysis time (16 min) and good repeatability and reproducibility values which are in most cases under 10% (RSD%). The accuracy values range from 95.4% to 111.3% whilst the LOQ values ranges from 0.05 to 0.29 mg/L depending on the analyte.
authors.affiliation: Dipartimento di Chimica, Università della Calabria, cubo 12/C, I-87036 Arcavacata di Rende, Cosenza, Italy. l.didonna@unical.it
authors.forename: Leonardo
authors.initials: L
authors.lastname: Di Donna
authors.forename: Domenico
authors.initials: D
authors.lastname: Taverna
authors.forename: Fabio
authors.initials: F
authors.lastname: Mazzotti
authors.forename: Hicham
authors.initials: H
authors.lastname: Benabdelkamel
authors.forename: Mohamed
authors.initials: M
authors.lastname: Attya
authors.forename: Anna
authors.initials: A
authors.lastname: Napoli
authors.forename: Giovanni
authors.initials: G
authors.lastname: Sindona
mesh_terms: D001628:Beverages / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002957:Citrus / Q000737:chemistry*; D044950:Flavanones / Q000737:chemistry*; D005638:Fruit / Q000737:chemistry; D015394:Molecular Structure; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D044950:Flavanones
keywords: Brutieridin; Derivatization chemistry; Flavonoids assay; Isotope dilution; Multiple reaction monitoring (MRM)