title: Karyotyping of diffuse large B-cell lymphomas: loss of 17p is associated with poor patient outcome.
abstract: BACKGROUND
  Cytogenetic studies of patients with diffuse large B-cell lymphoma (DLBCL) have revealed a large spectrum of chromosomal abnormalities, some of which may be clinically relevant. We wanted to evaluate possible associations between commonly acquired chromosome aberrations and prognosis in a large cohort of patients.
  
  
  METHODS
  All patients with DLBCL treated at our center during 1999-2010 with an abnormal G-banding karyotype determined on cells short-term cultured from diagnostic biopsies were included. Detailed information on staging, treatment, and outcome was available for all patients.
  
  
  RESULTS
  Of the 110 patients available for analysis, there were 48 deaths and 27 relapses after a median follow-up of 4.5 yr. Eleven different chromosomal abnormalities were detected in more than ten percent of patients. Of those, only loss of 17p, including the TP53 tumor suppressor gene, was significantly associated with inferior long-term prognosis. Five year overall and progression-free survival frequencies were 32% and 27% for patients with loss of 17p and 67% and 59% in patients without this abnormality.
  
  
  CONCLUSIONS
  In a relatively large cohort of patients with DLBCL analyzed by chromosome banding, loss of 17p was the only chromosomal abnormality associated with inferior survival in uni- and multivariate analysis.
authors.affiliation: Department of Immunology, Institute for Cancer Research, Oslo University Hospital, Oslo, Norway.
authors.forename: Idun
authors.initials: I
authors.lastname: Fiskvik
authors.forename: Hege V
authors.initials: HV
authors.lastname: Aamot
authors.forename: Jan
authors.initials: J
authors.lastname: Delabie
authors.forename: Erlend B
authors.initials: EB
authors.lastname: Smeland
authors.forename: Trond
authors.initials: T
authors.lastname: Stokke
authors.forename: Sverre
authors.initials: S
authors.lastname: Heim
authors.forename: Harald
authors.initials: H
authors.lastname: Holte
mesh_terms: D000293:Adolescent; D000368:Aged; D002869:Chromosome Aberrations; D002871:Chromosome Banding; D002886:Chromosomes, Human, Pair 17; D005260:Female; D006801:Humans; D007621:Karyotyping; D016403:Lymphoma, Large B-Cell, Diffuse / Q000175:diagnosis / Q000235:genetics* / Q000401:mortality / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D011379:Prognosis; D011446:Prospective Studies; D016019:Survival Analysis; D016159:Tumor Suppressor Protein p53 / Q000235:genetics*
chemical_list: C495901:TP53 protein, human; D016159:Tumor Suppressor Protein p53
keywords: 17p; TP53; diffuse large B-cell lymphoma; lymphoma karyotyping