title: White wine proteins: how does the pH affect their conformation at room temperature?
abstract: Our studies focused on the determination of aggregation mechanisms of proteins occurring in wine at room temperature. Even if the wine pH range is narrow (2.8 to 3.7), some proteins are affected by this parameter. At low pH, the formation of aggregates and the development of a haze due to proteins sometimes occur. The objective of this work was to determine if the pH impacted the conformational stability of wine proteins. Different techniques were used: circular dichroism and fluorescence spectroscopy to investigate the modification of their secondary and tertiary structure and also SAXS to determine their global shape. Four pure proteins were used, two considered to be stable (invertase and thaumatin-like proteins) and two considered to be unstable (two chitinase isoforms). Two pH values were tested to emphasize their behavior (pH 2.5 and 4.0). The present work highlighted the fact that the conformational stability of some wine proteins (chitinases) was impacted by partial modifications, related to the exposure of some hydrophobic sites. These modifications were enough to destabilize the native state of the protein. These modifications were not observed on wine proteins determined to be stable (invertase and thaumatin-like proteins).
authors.affiliation: INRA, UMR1083 SPO, F-34060 Montpellier, France.
authors.forename: Marie
authors.initials: M
authors.lastname: Dufrechou
authors.forename: Aude
authors.initials: A
authors.lastname: Vernhet
authors.forename: Pierre
authors.initials: P
authors.lastname: Roblin
authors.forename: François-Xavier
authors.initials: FX
authors.lastname: Sauvage
authors.forename: Céline
authors.initials: C
authors.lastname: Poncet-Legrand
mesh_terms: D002942:Circular Dichroism; D006863:Hydrogen-Ion Concentration; D011487:Protein Conformation; D011506:Proteins / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013696:Temperature; D014920:Wine / Q000032:analysis*
chemical_list: D011506:Proteins