title: Analysis and detection of the chemical constituents of Radix Polygalae and their metabolites in rats after oral administration by ultra high-performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry.
abstract: Radix Polygalae (RP), the dried root of Polygala tenuifolia Willd., is a well-known traditional Chinese medicine to mediate sedative, antipsychotic, cognitive improving, neuroprotective, and anti-inflammatory therapeutic effects on the central nervous system. In this work, ultra high-performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry (UHPLC/ESI-Q-TOF-MS/MS) was established for the separation and characterization of the chemical constituents in Radix Polygalae and their metabolites in rat plasma and urine after oral administration. Samples were separated on an Agilent Zorbax Eclipse Plus-C18 column (100mm×2.1mm, 1.8μm) with 0.1% formic acid aqueous solution and acetonitrile as the mobile phase under gradient conditions. Overall, 50 compounds were characterized from the RP, 9 of which are to our knowledge reported for the first time. In vivo, 10 components and 2 metabolites were observed in rat plasma, and 27 components and 7 metabolites were detected in rat urine. The results from this work improve our understanding on the chemical constituents of RP and their metabolic profiling.
authors.affiliation: Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, People's Republic of China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Ling
authors.forename: Zhixiong
authors.initials: Z
authors.lastname: Li
authors.forename: Mingcang
authors.initials: M
authors.lastname: Chen
authors.forename: Zhaolin
authors.initials: Z
authors.lastname: Sun
authors.forename: Mingsong
authors.initials: M
authors.lastname: Fan
authors.forename: Chenggang
authors.initials: C
authors.lastname: Huang
mesh_terms: D000284:Administration, Oral; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000032:analysis* / Q000378:metabolism*; D008297:Male; D009844:Oligosaccharides / Q000032:analysis; D031801:Polygala / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012503:Saponins / Q000032:analysis; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods*; D044004:Xanthones / Q000032:analysis
chemical_list: D004365:Drugs, Chinese Herbal; D009844:Oligosaccharides; D012503:Saponins; D044004:Xanthones; C009689:xanthone
keywords: Chemical constituents; Metabolites; Radix Polygalae; UHPLC/ESI-Q-TOF-MS