title: A new pyruvate oxidase biosensor based on 3-mercaptopropionic acid/6-aminocaproic acid modified gold electrode.
abstract: In the biosensor construction, 3-mercaptopropionic acid (3-MPA) and 6-aminocaproic acid (6-ACA) were used for forming self-assembled monolayer (SAM) on a gold disc electrode and pyruvate oxidase was immobilized on the modified electrode surface by using glutaraldehyde. Biosensor response is linearly related to pyruvate concentration at 2.5-50 μM, detection limit is 1.87 μM and response time of the biosensor is 6 s for differential pulse voltammograms. From the repeatability studies (n = 6) for 30.0 μM pyruvate revealed that the average value ([Formula: see text]), standard deviation (S.D) and coefficient of variation (CV %) were calculated to be 31.02 μM, ± 0.1914 μM and 0.62%, respectively.
authors.affiliation: Faculty of Science, Department of Biochemistry, Ege University , Bornova-İzmir , Turkey.
authors.forename: Ezgi
authors.initials: E
authors.lastname: Bayram
authors.forename: Erol
authors.initials: E
authors.lastname: Akyilmaz
mesh_terms: D015097:3-Mercaptopropionic Acid / Q000737:chemistry; D015119:Aminocaproic Acid / Q000737:chemistry; D015374:Biosensing Techniques; D055664:Electrochemical Techniques; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000737:chemistry*; D006046:Gold; D006801:Humans; D011771:Pyruvate Oxidase / Q000737:chemistry*
chemical_list: D004800:Enzymes, Immobilized; D006046:Gold; D015097:3-Mercaptopropionic Acid; D011771:Pyruvate Oxidase; D015119:Aminocaproic Acid
keywords: 3-mercaptopropionic acid; 6-aminocaproic acid; biosensor; pyruvate; pyruvate oxidase