title: Differential sensitivities of the growth of Escherichia coli to acrylate under aerobic and anaerobic conditions and its effect on product formation.
abstract: The effect of acrylate on the growth of Escherichia coli was determined under aerobic and anaerobic conditions in glucose-defined medium. Growth occurred with up to 35 mM acrylate under aerobic conditions but ceased at 5 mM acrylate under anaerobic conditions. This differential sensitivity can be attributed to inhibition of pyruvate formate lyase and/or pflB gene repression, as this enzyme is necessary for anaerobic growth of E. coli. The effect of acrylate on end-product distribution was also determined by growing E. coli first aerobically, then switching to anaerobic conditions. In the absence of acrylate, E. coli generated the typical distribution of mixed-acid products, with about 12 % of pyruvate being metabolically converted to lactate. In contrast, in the presence of 5 mM acrylate, E. coli converted 83 % of pyruvate to lactate, consistent with a reduction in pyruvate formate lyase activity.
authors.affiliation: BioChemical Engineering, College of Engineering, University of Georgia, Athens, GA, 30602, USA.
authors.forename: Ajay S
authors.initials: AS
authors.lastname: Arya
authors.forename: Sarah A
authors.initials: SA
authors.lastname: Lee
authors.forename: Mark A
authors.initials: MA
authors.lastname: Eiteman
mesh_terms: D000123:Acetyltransferases / Q000378:metabolism; D000179:Acrylates / Q000633:toxicity*; D000332:Aerobiosis; D000693:Anaerobiosis; D003470:Culture Media / Q000737:chemistry; D004926:Escherichia coli / Q000187:drug effects* / Q000254:growth & development* / Q000502:physiology; D005947:Glucose / Q000378:metabolism; D019344:Lactic Acid / Q000378:metabolism; D019289:Pyruvic Acid / Q000378:metabolism
chemical_list: D000179:Acrylates; D003470:Culture Media; D019344:Lactic Acid; D019289:Pyruvic Acid; D000123:Acetyltransferases; C022560:formate C-acetyltransferase; D005947:Glucose; C036658:acrylic acid