title: Biocalcification using B. pasteurii for strengthening brick masonry civil engineering structures.
abstract: Microbiologically induced calcite precipitation in bricks by bacterium Bacillus pasteurii (NCIM 2477) using a media especially optimized for urease production (OptU) was demonstrated in this study. Effect of biocalcification activity on compressive strength and water absorption capacity of bricks was investigated. Various other parameters such as pH, growth profile, urease activity, urea breakdown and calcite precipitated were monitored during the 28 days curing period. Efficiency of B. pasteurii to form microbial aided calcite precipitate in OptU media resulted into 83.9% increase in strength of the bricks as compared to only 24.9% with standard media, nutrient broth (NB). In addition to significant increase in the compressive strength, bricks treated with B. pasteurii grown in OptU media resulted in 48.9 % reduction in water absorption capacity as compared to control bricks immersed in tap water. Thus it was successfully demonstrated that microbial calcification in optimized media by Bacillus pasteurii has good potential for commercial application to improve the life span of structures constructed with bricks, particularly structures of heritage importance.
authors.affiliation: Department of Food Engineering and Technology, Institute of Chemical Technology, N. P. Marg, Matunga, Mumbai, 400019, India.
authors.forename: Supriya H
authors.initials: SH
authors.lastname: Raut
authors.forename: D D
authors.initials: DD
authors.lastname: Sarode
authors.forename: S S
authors.initials: SS
authors.lastname: Lele
mesh_terms: D001407:Bacillus / Q000201:enzymology / Q000378:metabolism*; D001709:Biotechnology / Q000379:methods*; D002119:Calcium Carbonate / Q000378:metabolism*; D003470:Culture Media / Q000737:chemistry; D004738:Engineering / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D013696:Temperature; D014508:Urea / Q000378:metabolism; D014510:Urease / Q000378:metabolism
chemical_list: D003470:Culture Media; D014508:Urea; D014510:Urease; D002119:Calcium Carbonate