title: Effects of human epidermal growth factor gene-transfected mesenchymal stem cells on fibroblast migration and proliferation.
abstract: OBJECTIVE
  We were interested in determining whether epidermal growth factor gene-transfected mesenchymal stem cells (EGF-MSC) would accelerate fibroblast migration and proliferation.
  
  
  METHODS
  Fibroblasts were cultured in serum-free conditioned media from EGF-MSC; RT-PCR was performed to detect expression of EGF gene in EGF-MSCs. EGF protein levels in cell culture supernatants from EGF-MSC were assayed by ELISA and proliferation of EGF-MSC-treated fibroblasts was performed using MTT assay. Effects of EGF-MSC on fibroblast migration were evaluated using scratch wound and transmigration assays. Cell adhesion molecules, cell dynamics molecules and phospho-(Ser) kinase substrate expressions of EGF-MSC-treated fibroblasts were evaluated by western blotting.
  
  
  RESULTS
  EGF gene expression increased in EGF-MSCs and viability of EGF-MSC-treated fibroblasts was elevated. EGF-MSC-treated fibroblasts showed increased migration compared to controls. Expressions of cell adhesion molecules (β-catenin, N-cadherin), cell dynamics molecules (cofilin, ezrin) and phospho-(Ser) kinase substrates (phospho-MAPK/CDK substrate, phospho-Arg-(Ser)-X-Tyr/Phe-X-pSer motif) increased in EGF-MSC-treated fibroblasts. These results imply that EGF-MSCs contributed to enhancing the wound healing process by increased cell adhesion, dynamic effects, fibroblast migration, and proliferation.
  
  
  CONCLUSIONS
  This study indicates that EGF-MSCs had a positive influence on fibroblast migration and proliferation and EGF-MSC may provide a useful strategy for wound healing.
authors.affiliation: Department of Biological Science, Gachon University of Medicine and Science, Incheon, 406-799, Korea.
authors.forename: D H
authors.initials: DH
authors.lastname: You
authors.forename: M J
authors.initials: MJ
authors.lastname: Nam
mesh_terms: D051339:Actin Depolymerizing Factors / Q000235:genetics / Q000378:metabolism; D015820:Cadherins / Q000235:genetics / Q000378:metabolism; D002448:Cell Adhesion / Q000235:genetics / Q000502:physiology; D048708:Cell Growth Processes / Q000235:genetics; D002465:Cell Movement / Q000235:genetics / Q000502:physiology*; D002478:Cells, Cultured; D017077:Culture Media, Conditioned; D018844:Cyclin-Dependent Kinases / Q000235:genetics / Q000378:metabolism; D003598:Cytoskeletal Proteins / Q000235:genetics / Q000378:metabolism; D004815:Epidermal Growth Factor / Q000235:genetics* / Q000378:metabolism*; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D020928:Mitogen-Activated Protein Kinases / Q000235:genetics / Q000378:metabolism; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism; D012867:Skin / Q000378:metabolism / Q000503:physiopathology; D014162:Transfection; D014945:Wound Healing / Q000235:genetics / Q000502:physiology; D051176:beta Catenin / Q000235:genetics / Q000378:metabolism; D000099149:Ezrin
chemical_list: D051339:Actin Depolymerizing Factors; D015820:Cadherins; D017077:Culture Media, Conditioned; D018844:Cyclin-Dependent Kinases; D003598:Cytoskeletal Proteins; D004815:Epidermal Growth Factor; D020928:Mitogen-Activated Protein Kinases; D017346:Protein Serine-Threonine Kinases; D051176:beta Catenin; D000099149:Ezrin