title: Structural characteristics and biological performance of silk fibroin nanofiber containing microalgae Spirulina extract.
abstract: Silk fibroin (SF) nanofiber scaffold containing microalgae Spirulina extract were prepared by electrospinning and the performance and functionality of the scaffold were evaluated. The viscosity and conductivity of the dope solution of Spirulina containing SF were examined for electrospinability and we found that the morphological structure of SF nanofiber is affected by the concentration of Spirulina extract added. The platelet adhesion and coagulation time test confirmed that the Spirulina containing SF nanofiber scaffold had excellent ability to prevent blood clotting or antithrombogenicity that is comparable to heparin. Low cytotoxicity and excellent cell adhesion and proliferation were also observed for Sprulina containing SF nanofiber scaffold by methylthiazolyldiphenyl-tetrazolium bromide assay and confocal fluorescence microscope using fibroblast and human umbilical vein endothelial cells. Based on these results, we believe SF nanofiber scaffold containing Spirulina extract has the potential to be used as tissue engineering scaffold that requires high hemocompatibility.
authors.affiliation: Department of Biosystems & Biomaterials Science and Engineering, Seoul National University, Seoul, Korea.
authors.forename: Bum-Gyu
authors.initials: BG
authors.lastname: Cha
authors.forename: Hyo Won
authors.initials: HW
authors.lastname: Kwak
authors.forename: A Reum
authors.initials: AR
authors.lastname: Park
authors.forename: Shin Hwan
authors.initials: SH
authors.lastname: Kim
authors.forename: Sook-Young
authors.initials: SY
authors.lastname: Park
authors.forename: Hyun-Jeong
authors.initials: HJ
authors.lastname: Kim
authors.forename: Ick-Soo
authors.initials: IS
authors.lastname: Kim
authors.forename: Ki Hoon
authors.initials: KH
authors.lastname: Lee
authors.forename: Young Hwan
authors.initials: YH
authors.lastname: Park
mesh_terms: D000818:Animals; D001777:Blood Coagulation / Q000187:drug effects; D012831:Bombyx; D002470:Cell Survival / Q000187:drug effects; D004553:Electric Conductivity; D005349:Fibroins / Q000737:chemistry* / Q000494:pharmacology*; D008422:Materials Testing; D058086:Microalgae / Q000737:chemistry*; D008856:Microscopy, Fluorescence; D057139:Nanofibers / Q000737:chemistry* / Q000648:ultrastructure; D010973:Platelet Adhesiveness / Q000187:drug effects; D011817:Rabbits; D012996:Solutions; D053545:Spirulina / Q000737:chemistry*; D014783:Viscosity
chemical_list: D012996:Solutions; D005349:Fibroins
keywords: Spirulina extract; microalgae; nanofiber; scaffold; silk fibroin