title: Pyruvate production in Candida glabrata: manipulation and optimization of physiological function.
abstract: Candida glabrata, a multi-vitamin auxotrophic yeast, can accumulate a large amount of pyruvate extracellularly using glucose as the carbon source, a characteristic that has facilitated the cost-effective biotechnological production of pyruvate on an industrial scale. In this review, we describe the current advances in further improving the performance of C. glabrata for efficient pyruvate production, which includes: optimization of the vitamin and dissolved oxygen concentrations, regulation of intracellular cofactor levels and improvement of the environmental robustness of C. glabrata. We also discuss the current efforts using systems biology to understand the metabolism of C. glabrata. Finally, perspectives on engineering and exploiting C. glabrata as a cell factory for efficiently producing various chemicals and materials are discussed.
authors.affiliation: a State Key Laboratory of Food Science and Technology and.|b The Key Laboratory of Industrial Biotechnology , Ministry of Education, Jiangnan University , Wuxi , Jiangsu , China.
authors.forename: Shubo
authors.initials: S
authors.lastname: Li
authors.affiliation: a State Key Laboratory of Food Science and Technology and.|b The Key Laboratory of Industrial Biotechnology , Ministry of Education, Jiangnan University , Wuxi , Jiangsu , China.
authors.forename: Xiulai
authors.initials: X
authors.lastname: Chen
authors.affiliation: a State Key Laboratory of Food Science and Technology and.|b The Key Laboratory of Industrial Biotechnology , Ministry of Education, Jiangnan University , Wuxi , Jiangsu , China.
authors.forename: Liming
authors.initials: L
authors.lastname: Liu
authors.affiliation: a State Key Laboratory of Food Science and Technology and.|b The Key Laboratory of Industrial Biotechnology , Ministry of Education, Jiangnan University , Wuxi , Jiangsu , China.
authors.forename: Jian
authors.initials: J
authors.lastname: Chen
mesh_terms: D001709:Biotechnology; D041221:Candida glabrata / Q000235:genetics / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism; D010100:Oxygen / Q000378:metabolism; D019289:Pyruvic Acid / Q000378:metabolism*; D049490:Systems Biology; D014815:Vitamins / Q000378:metabolism
chemical_list: D014815:Vitamins; D019289:Pyruvic Acid; D005947:Glucose; D010100:Oxygen
keywords: Candida glabrata; dissolved oxygen; environmental robustness; intracellular cofactors; pyruvate; systems biology