title: An oral vaccine against candidiasis generated by a yeast molecular display system.
abstract: Enolase 1 (Eno1p) of Candida albicans is an immunodominant antigen. However, conventional technologies for preparing an injectable vaccine require purification of the antigenic protein and preparation of an adjuvant. To develop a novel type of oral vaccine against candidiasis, we generated Saccharomyces cerevisiae cells that display the Eno1p antigen on their surfaces. Oral delivery of the engineered S. cerevisiae cells prolonged survival rate of mice that were subsequently challenged with C. albicans. Given that a vaccine produced using molecular display technology avoids the need for protein purification, this oral vaccine offers a promising alternative to the use of conventional and injectable vaccines for preventing a range of infectious diseases.
authors.affiliation: General Education Center and Gradudate School of Pharmacy, Hyogo University of Health Sciences, Kobe, Japan.
authors.forename: Seiji
authors.initials: S
authors.lastname: Shibasaki
authors.forename: Wataru
authors.initials: W
authors.lastname: Aoki
authors.forename: Takashi
authors.initials: T
authors.lastname: Nomura
authors.forename: Ayuko
authors.initials: A
authors.lastname: Miyoshi
authors.forename: Senji
authors.initials: S
authors.lastname: Tafuku
authors.forename: Tomomitsu
authors.initials: T
authors.lastname: Sewaki
authors.forename: Mitsuyoshi
authors.initials: M
authors.lastname: Ueda
mesh_terms: D000284:Administration, Oral; D000818:Animals; D002176:Candida albicans / Q000235:genetics / Q000276:immunology*; D002177:Candidiasis / Q000276:immunology / Q000401:mortality / Q000517:prevention & control*; D061505:Cell Surface Display Techniques; D004195:Disease Models, Animal; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005260:Female; D005656:Fungal Proteins / Q000235:genetics / Q000276:immunology; D005657:Fungal Vaccines / Q000008:administration & dosage* / Q000276:immunology; D051379:Mice; D011994:Recombinant Proteins; D020798:Two-Hybrid System Techniques; D014611:Vaccination
chemical_list: D005656:Fungal Proteins; D005657:Fungal Vaccines; D011994:Recombinant Proteins
keywords: Candida albicans; Eno1p; Saccharomyces cerevisiae; cell surface engineering