title: Preparation, modification, and characterization of alginate hydrogel with nano-/microfibers: a new perspective for tissue engineering.
abstract: We aimed to develop an alginate hydrogel (AH) modified with nano-/microfibers of titanium dioxide (nfTD) and hydroxyapatite (nfHY) and evaluated its biological and chemical properties. Nano-/microfibers of nfTD and nfHY were combined with AH, and its chemical properties were evaluated by FTIR spectroscopy, X-ray diffraction, energy dispersive X-Ray analysis, and the cytocompatibility by the WST-1 assay. The results demonstrate that the association of nfTD and nfHY nano-/microfibers to AH did not modified the chemical characteristics of the scaffold and that the association was not cytotoxic. In the first 3 h of culture with NIH/3T3 cells nfHY AH scaffolds showed a slight increase in cell viability when compared to AH alone or associated with nfTD. However, an increase in cell viability was observed in 24 h when nfTD was associated with AH scaffold. In conclusion our study demonstrates that the combination of nfHY and nfTD nano-/microfibers in AH scaffold maintains the chemical characteristics of alginate and that this association is cytocompatible. Additionally the combination of nfHY with AH favored cell viability in a short term, and the addition of nfTD increased cell viability in a long term.
authors.affiliation: Nucleus of Cellular and Tecidual Biology (NCTBio), Post-Graduate Program in Dentistry, Federal University of Pelotas, Pelotas, RS, Brazil. biancapalmasantana.mg@gmail.com
authors.forename: Bianca Palma
authors.initials: BP
authors.lastname: Santana
authors.forename: Fernanda
authors.initials: F
authors.lastname: Nedel
authors.forename: Evandro
authors.initials: E
authors.lastname: Piva
authors.forename: Rodrigo Varella
authors.initials: RV
authors.lastname: de Carvalho
authors.forename: Flávio Fernando
authors.initials: FF
authors.lastname: Demarco
authors.forename: Neftali Lenin Villarreal
authors.initials: NL
authors.lastname: Carreño
mesh_terms: D000464:Alginates / Q000138:chemical synthesis* / Q000494:pharmacology; D000818:Animals; D016923:Cell Death / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D017886:Durapatite / Q000494:pharmacology; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects; D020723:Glucuronic Acid / Q000138:chemical synthesis / Q000494:pharmacology; D006603:Hexuronic Acids / Q000138:chemical synthesis / Q000494:pharmacology; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000138:chemical synthesis* / Q000494:pharmacology; D008422:Materials Testing; D051379:Mice; D041681:NIH 3T3 Cells; D057139:Nanofibers / Q000737:chemistry*; D013052:Spectrometry, X-Ray Emission; D017550:Spectroscopy, Fourier Transform Infrared; D023822:Tissue Engineering / Q000379:methods*; D014025:Titanium / Q000494:pharmacology; D014961:X-Ray Diffraction
chemical_list: D000464:Alginates; D006603:Hexuronic Acids; C009495:titanium dioxide; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D020723:Glucuronic Acid; D017886:Durapatite; D014025:Titanium