title: [Control of toxicity of Sarcocystis fayeri in horsemeat by freezing treatment and prevention of food poisoning caused by raw consumption of horsemeat].
abstract: More than 27 outbreaks per year of food poisoning caused by consuming horse meat were reported in Kumamoto Prefecture (including Kumamoto City) from January 2009 to September 2011. It was found that the causative agent of the outbreaks was a protein with a molecular weight of 15 kDa that had originated from bradyzoites of Sarcocystis fayeri parasitizing the horse meat. Rabit ileal loop tests showed that pepsin treatment of homogenates of frozen horse meat containing the cysts of S. fayeri induced loss of toxicity, presumably by digestion of the proteinous causative agent(s). Slices of horse meat containing the cysts were frozen at below -20°C for various periods. The cysts were collected after thawing the slices, then treated in an artificial stomach juice containing pepsin. The bradyzoites of the cysts kept at -20°C for 48 hr or more completely disappeared. Simultaneously, the 15 kDa protein also disappeared in the frozen cysts. After notifying the public and recommending freezing treatment of horse meat, no subsequent cases of food poisoning were reported. This indicates that freezing of horse meat is effective to prevent the occurrence of food poisoning caused by consuming raw horse meat containing S. fayeri.
authors.affiliation: Kumamoto Prefectural Institute of Public Health and Environmental Science, 1240-1 Kurizaki-machi, Uto-shi, Kumamoto 869-0425, Japan.
authors.forename: Seiya
authors.initials: S
authors.lastname: Harada
authors.forename: Masato
authors.initials: M
authors.lastname: Furukawa
authors.forename: Eisuke
authors.initials: E
authors.lastname: Tokuoka
authors.forename: Kazutoshi
authors.initials: K
authors.lastname: Matsumoto
authors.forename: Shunsuke
authors.initials: S
authors.lastname: Yahiro
authors.forename: Jiro
authors.initials: J
authors.lastname: Miyasaka
authors.forename: Morihiro
authors.initials: M
authors.lastname: Saito
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Kamata
authors.forename: Maiko
authors.initials: M
authors.lastname: Watanabe
authors.forename: Daisuke
authors.initials: D
authors.lastname: Irikura
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Matsumoto
authors.forename: Yoshiko
authors.initials: Y
authors.lastname: Sugita-Konishi
mesh_terms: D000818:Animals; D005511:Food Handling / Q000379:methods*; D005517:Foodborne Diseases / Q000453:epidemiology / Q000469:parasitology* / Q000517:prevention & control*; D005615:Freezing; D006736:Horses; D006801:Humans; D007564:Japan / Q000453:epidemiology; D008460:Meat / Q000469:parasitology* / Q000506:poisoning*; D011817:Rabbits; D012522:Sarcocystis / Q000302:isolation & purification / Q000472:pathogenicity*; D012523:Sarcocystosis / Q000469:parasitology* / Q000517:prevention & control*