title: A low-cost culture medium for the production of Nannochloropsis gaditana biomass optimized for aquaculture.
abstract: Nannochloropsis gaditana is a microalga with a high nutritional value and a protein and polyunsaturated fatty acid (PUFA) content that makes it interesting as a feed in aquaculture. To maximize its productivity and nutritional value in large-scale culture, a well-known commercial medium was optimized to the most favorable nutrient level using commercial fertilizers. Optimal growth conditions were obtained in the alternative fertilizer-based medium at a nitrogen concentration of 11.3 mM, a phosphorus concentration of 0.16 mM, and a micronutrient concentration of 30 μL L(-1). This alternative medium allowed to obtain a biomass concentration similar to that achieved when using the commercial formula but with a reduction in Cu, Fe, and Mo content of 71%, 89%, and 99%, respectively. A maximum biomass productivity of 0.51 g L(-1) d(-1) was obtained. The eicosapentaenoic acid and protein contents of the biomass were 2.84% and 44% of dry weight, respectively.
authors.affiliation: Chemical Engineering Area, Department of Engineering, University of Almería, E04120 Almería, Spain.
authors.forename: J
authors.initials: J
authors.lastname: Camacho-Rodríguez
authors.forename: M C
authors.initials: MC
authors.lastname: Cerón-García
authors.forename: C V
authors.initials: CV
authors.lastname: González-López
authors.forename: J M
authors.initials: JM
authors.lastname: Fernández-Sevilla
authors.forename: A
authors.initials: A
authors.lastname: Contreras-Gómez
authors.forename: E
authors.initials: E
authors.lastname: Molina-Grima
mesh_terms: D000383:Agriculture; D017756:Aquaculture; D061249:Batch Cell Culture Techniques; D018533:Biomass; D018929:Cell Culture Techniques / Q000191:economics* / Q000379:methods*; D003365:Costs and Cost Analysis; D003470:Culture Media / Q000737:chemistry / Q000191:economics* / Q000494:pharmacology*; D005227:Fatty Acids / Q000378:metabolism; D005308:Fertilizers; D018977:Micronutrients / Q000032:analysis; D009584:Nitrogen / Q000032:analysis; D010758:Phosphorus / Q000032:analysis; D058009:Stramenopiles / Q000166:cytology / Q000254:growth & development*
chemical_list: D003470:Culture Media; D005227:Fatty Acids; D005308:Fertilizers; D018977:Micronutrients; D010758:Phosphorus; D009584:Nitrogen
keywords: Aquaculture; EPA; Medium optimization; Nannochloropsis gaditana; Proteins