title: An automated robotic platform for rapid profiling oligosaccharide analysis of monoclonal antibodies directly from cell culture.
abstract: Oligosaccharides attached to Asn297 in each of the CH2 domains of monoclonal antibodies play an important role in antibody effector functions by modulating the affinity of interaction with Fc receptors displayed on cells of the innate immune system. Rapid, detailed, and quantitative N-glycan analysis is required at all stages of bioprocess development to ensure the safety and efficacy of the therapeutic. The high sample numbers generated during quality by design (QbD) and process analytical technology (PAT) create a demand for high-performance, high-throughput analytical technologies for comprehensive oligosaccharide analysis. We have developed an automated 96-well plate-based sample preparation platform for high-throughput N-glycan analysis using a liquid handling robotic system. Complete process automation includes monoclonal antibody (mAb) purification directly from bioreactor media, glycan release, fluorescent labeling, purification, and subsequent ultra-performance liquid chromatography (UPLC) analysis. The entire sample preparation and commencement of analysis is achieved within a 5-h timeframe. The automated sample preparation platform can easily be interfaced with other downstream analytical technologies, including mass spectrometry (MS) and capillary electrophoresis (CE), for rapid characterization of oligosaccharides present on therapeutic antibodies.
authors.affiliation: National Institute of Bioprocessing Research and Training (NIBRT), Mount Merrion, Blackrock, Dublin 4, Ireland. Electronic address: margaret.doherty@nibrt.ie.
authors.forename: Margaret
authors.initials: M
authors.lastname: Doherty
authors.forename: Jonathan
authors.initials: J
authors.lastname: Bones
authors.forename: Niaobh
authors.initials: N
authors.lastname: McLoughlin
authors.forename: Jayne E
authors.initials: JE
authors.lastname: Telford
authors.forename: Bryan
authors.initials: B
authors.lastname: Harmon
authors.forename: Michael R
authors.initials: MR
authors.lastname: DeFelippis
authors.forename: Pauline M
authors.initials: PM
authors.lastname: Rudd
mesh_terms: D000911:Antibodies, Monoclonal / Q000737:chemistry*; D001331:Automation; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D003470:Culture Media / Q000737:chemistry*; D057166:High-Throughput Screening Assays; D006801:Humans; D008958:Models, Molecular; D009844:Oligosaccharides / Q000032:analysis*; D012371:Robotics
chemical_list: D000911:Antibodies, Monoclonal; D003470:Culture Media; D009844:Oligosaccharides
keywords: Automation; Cell culture media; Glycomics; Monoclonal antibodies; Oligosaccharides; Ultra-performance liquid chromatography