title: Studies on the assembly characteristics of large subunit ribosomal proteins in S. cerevisae.
abstract: During the assembly process of ribosomal subunits, their structural components, the ribosomal RNAs (rRNAs) and the ribosomal proteins (r-proteins) have to join together in a highly dynamic and defined manner to enable the efficient formation of functional ribosomes. In this work, the assembly of large ribosomal subunit (LSU) r-proteins from the eukaryote S. cerevisiae was systematically investigated. Groups of LSU r-proteins with specific assembly characteristics were detected by comparing the protein composition of affinity purified early, middle, late or mature LSU (precursor) particles by semi-quantitative mass spectrometry. The impact of yeast LSU r-proteins rpL25, rpL2, rpL43, and rpL21 on the composition of intermediate to late nuclear LSU precursors was analyzed in more detail. Effects of these proteins on the assembly states of other r-proteins and on the transient LSU precursor association of several ribosome biogenesis factors, including Nog2, Rsa4 and Nop53, are discussed.
authors.affiliation: Lehrstuhl für Biochemie III, Universität Regensburg, Regensburg, Germany.
authors.forename: Uli
authors.initials: U
authors.lastname: Ohmayer
authors.forename: Michael
authors.initials: M
authors.lastname: Gamalinda
authors.forename: Martina
authors.initials: M
authors.lastname: Sauert
authors.forename: Julius
authors.initials: J
authors.lastname: Ossowski
authors.forename: Gisela
authors.initials: G
authors.lastname: Pöll
authors.forename: Jan
authors.initials: J
authors.lastname: Linnemann
authors.forename: Thomas
authors.initials: T
authors.lastname: Hierlmeier
authors.forename: Jorge
authors.initials: J
authors.lastname: Perez-Fernandez
authors.forename: Beril
authors.initials: B
authors.lastname: Kumcuoglu
authors.forename: Isabelle
authors.initials: I
authors.lastname: Leger-Silvestre
authors.forename: Marlène
authors.initials: M
authors.lastname: Faubladier
authors.forename: Joachim
authors.initials: J
authors.lastname: Griesenbeck
authors.forename: John
authors.initials: J
authors.lastname: Woolford
authors.forename: Herbert
authors.initials: H
authors.lastname: Tschochner
authors.forename: Philipp
authors.initials: P
authors.lastname: Milkereit
mesh_terms: D002846:Chromatography, Affinity; D013058:Mass Spectrometry; D055503:Protein Multimerization / Q000502:physiology*; D020543:Proteome / Q000032:analysis; D040901:Proteomics / Q000379:methods; D012322:RNA Precursors / Q000032:analysis / Q000378:metabolism; D012323:RNA Processing, Post-Transcriptional; D012269:Ribosomal Proteins / Q000737:chemistry / Q000378:metabolism*; D054658:Ribosome Subunits, Large / Q000737:chemistry / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000737:chemistry / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000378:metabolism
chemical_list: D020543:Proteome; D012322:RNA Precursors; D012269:Ribosomal Proteins; D029701:Saccharomyces cerevisiae Proteins