title: Antihemolytic activity and mineral contents of Juglans regia L. flowers.
abstract: OBJECTIVE
  Juglans (J.) regia L. is known to possess many biological properties. In this study, antihemolytic activity of methanol extract of Juglans regia L. flower were investigated.
  
  
  METHODS
  Antihemolytic activities of Juglans regia L. flowers were evaluated by various in vitro assays. In addition, scavenging of hydrogen peroxide and mineral contents of flowers were determined using atomic absorption spectroscopy.
  
  
  RESULTS
  Extract showed good antihemolytic activity against H2O2 and CuOOH induced hemolysis in comparison with control. Extract was capable of scavenging H2O2 in a concentration dependent manner. IC50 for H2O2 scavenging activity was 311±12.8 µg ml-1. The amount of eight elements was determined and was in the order: Mn > Cu > Fe > Zn.
  
  
  CONCLUSIONS
  Our study indicate that J. regia flower has remarkable antihemolytic activity, which maybe result of its high phenol and flavonoid contents, especially quercetin.
authors.affiliation: Thalassaemia Research Center, Mazandaran University of Medical Sciences, Sari, Iran. zadeh20@yahoo.com
authors.forename: M A
authors.initials: MA
authors.lastname: Ebrahimzadeh
authors.forename: S F
authors.initials: SF
authors.lastname: Nabavi
authors.forename: S M
authors.initials: SM
authors.lastname: Nabavi
mesh_terms: D000818:Animals; D004912:Erythrocytes / Q000187:drug effects; D005419:Flavonoids / Q000032:analysis; D035264:Flowers / Q000737:chemistry*; D016166:Free Radical Scavengers; D005612:Freeze Drying; D006461:Hemolysis / Q000187:drug effects*; D006861:Hydrogen Peroxide / Q000737:chemistry; D066298:In Vitro Techniques; D031324:Juglans / Q000737:chemistry*; D008297:Male; D008670:Metals / Q000032:analysis; D008903:Minerals / Q000032:analysis*; D010636:Phenols / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar
chemical_list: D005419:Flavonoids; D016166:Free Radical Scavengers; D008670:Metals; D008903:Minerals; D010636:Phenols; D006861:Hydrogen Peroxide