title: Injury-dependent retention of intraportally administered mesenchymal stromal cells following partial hepatectomy of steatotic liver does not lead to improved liver recovery.
abstract: The aim of this study was to evaluate the effect of bone marrow-derived mesenchymal stromal cell (BM-MSC) administration on liver function following partial hepatectomy (PHx) of methionine/choline-deficient (MCD) diet induced steatotic livers in rodents. Here we identified and validated serum cholinesterase (CHE) and triglyceride (TG) levels as non-invasive markers to longitudinally monitor rat liver function. Using in vivo bioluminescence imaging, retention of BM-MSC in the liver was observed following intraportal administration, but not after intravenous administration. Therefore, BM-MSC were intraportally delivered to investigate the effect on liver recovery and/or regeneration after PHx. However, despite recovery to normal body weight, liver weight and NAS score, both serum CHE and TG levels of non-treated and cell-treated rats with PHx after MCD diet remained significantly lower as compared to those of control rats. Importantly, serum CHE levels, but not TG levels, of cell-treated rats remained significantly lower as compared to those of non-treated rats, thereby warranting that certain caution should be considered for future clinical application of IP BM-MSC administration in order to promote liver regeneration and/or function.
authors.affiliation: Laboratory of Experimental Surgery, Antwerp Surgical Training and Research Centre, University of Antwerp/University Hospital of Antwerp, Antwerp, Belgium.
authors.forename: Nele
authors.initials: N
authors.lastname: Boeykens
authors.forename: Peter
authors.initials: P
authors.lastname: Ponsaerts
authors.forename: Annemie
authors.initials: A
authors.lastname: Van der Linden
authors.forename: Zwi
authors.initials: Z
authors.lastname: Berneman
authors.forename: Dirk
authors.initials: D
authors.lastname: Ysebaert
authors.forename: Kathleen
authors.initials: K
authors.lastname: De Greef
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D015415:Biomarkers / Q000097:blood*; D001835:Body Weight; D002796:Choline Deficiency; D002802:Cholinesterases / Q000097:blood; D017931:DNA Primers / Q000235:genetics; D005234:Fatty Liver / Q000209:etiology / Q000503:physiopathology* / Q000601:surgery*; D005260:Female; D005434:Flow Cytometry; D006498:Hepatectomy / Q000379:methods; D016130:Immunophenotyping; D008115:Liver Regeneration / Q000502:physiology*; D008163:Luminescent Measurements; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D008715:Methionine / Q000172:deficiency; D009929:Organ Size; D051381:Rats; D011917:Rats, Inbred Lew; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014280:Triglycerides / Q000097:blood
chemical_list: D015415:Biomarkers; D017931:DNA Primers; D014280:Triglycerides; D008715:Methionine; D002802:Cholinesterases