title: [RNAi-mediated knockdown of cyclooxygenase 2 inhibits the growth and migration of SaOS2 human osteosarcoma cells].
abstract: OBJECTIVE
  To explore the effects of a knockdown of cyclooxygenase 2 upon the growth and migration of SaOS2 cells.
  
  
  METHODS
  We employed lentivirus mediated-RNA interference technology to knockdown the endogenous expression of gene COX-2 in human osteosarcoma cells (SaOS2) and analyzed their phenotypical changes. The effects of COX-2 silencing on the proliferation, cell cycle and migration of SaOS2 cells were assessed by thiazolyl blue tetrazolium bromide, flow cytometry and migration assays respectively. COX-2, vascular endothelial growth factor (VEGF), epidermal growth factor (EGF), basic fibroblast growth factor (bFGF) mRNA and protein expression were detected by reverse transcription-polymerase chain reaction (RT-PCR) and Western blot.
  
  
  RESULTS
  A decreased expression of COX-2 in human osteosarcoma cells significantly inhibited the growth and decreased the migration ability of SaOS2 cells. Furthermore, it also reduced VEGF, EGF and bFGF mRNA and protein expression.
  
  
  CONCLUSIONS
  The COX-2 signaling pathway may provide a novel therapeutic target for the treatment of human osteosarcoma.
authors.affiliation: Department of Orthopedics, Fuyang Cancer Hospital, Anhui 236000, China.
authors.forename: Zhou
authors.initials: Z
authors.lastname: Liu
authors.forename: Xing-zhou
authors.initials: XZ
authors.lastname: Wu
authors.forename: Rui
authors.initials: R
authors.lastname: Song
authors.forename: Xun-fu
authors.initials: XF
authors.lastname: Zong
authors.forename: Lei
authors.initials: L
authors.lastname: Ru
authors.forename: Kai
authors.initials: K
authors.lastname: Li
authors.forename: Qing-hua
authors.initials: QH
authors.lastname: Zhao
mesh_terms: D001859:Bone Neoplasms / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D051546:Cyclooxygenase 2 / Q000378:metabolism*; D004815:Epidermal Growth Factor / Q000378:metabolism; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism; D055785:Gene Knockdown Techniques; D006801:Humans; D012516:Osteosarcoma / Q000235:genetics / Q000378:metabolism*; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics; D015398:Signal Transduction; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism
chemical_list: D034741:RNA, Small Interfering; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A; D016222:Fibroblast Growth Factor 2; D004815:Epidermal Growth Factor; D051546:Cyclooxygenase 2; C496540:PTGS2 protein, human