title: PCR-RFLP-based typing for differentiation of Tomato yellow leaf curl virus (TYLCV) genotypes from infected host plants in Korea.
abstract: A polymerase chain reaction (PCR) using two sets of primers designed from published Tomato yellow leaf curl virus (TYLCV) genomes was developed to distinguish from the TYLCV-IL groups. The specificity of the two sets of primers was proven by testing against control TYLCV genomes and the symptomatic leaves of 34 different tomato cultivars naturally infected with TYLCV in greenhouses. One set for TYLCV-IL strain-specific primers (TYLCV-UNI-F and TYLCV-UNI-R) amplified full-length genome fragments from all the 34 tomato cultivars. Another set for TYLCV-IL group-II strain-specific primers (TYLCV-GPII-F and TYLCV-GPII-R) amplified target DNA fragments from only 9 tomato cultivars. Digestion by BglII and EcoRV of the PCR amplicons produced restriction fragment length polymorphism pattern that distinguished the TYLCV-IL group-I with two fragments from the TYLCV-IL group-II with no digested fragment. PCR coupled with BglII and EcoRV digestion confirmed that the 9 tomato cultivars were infected with the TYLCV-IL group-II and the remained 25 tomato cultivars were infected with the TYLCV-IL group-I.
authors.affiliation: Department of Biology and Medicinal Science, Pai Chai University, Daejeon, 302-735, Korea.
authors.forename: Sung
authors.initials: S
authors.lastname: Oh
authors.forename: Seongdae
authors.initials: S
authors.lastname: Kim
authors.forename: Nagarajan
authors.initials: N
authors.lastname: Vinod
authors.forename: Jung Mo
authors.initials: JM
authors.lastname: Koo
authors.forename: Kyung Min
authors.initials: KM
authors.lastname: Jang
authors.forename: Chang Won
authors.initials: CW
authors.lastname: Choi
authors.forename: Seong Hwan
authors.initials: SH
authors.lastname: Kim
authors.forename: Young Shik
authors.initials: YS
authors.lastname: Kim
mesh_terms: D053839:Begomovirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D017931:DNA Primers / Q000235:genetics; D018551:Solanum lycopersicum / Q000821:virology*; D010935:Plant Diseases / Q000821:virology*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length; D056910:Republic of Korea
chemical_list: D017931:DNA Primers