title: Genetic assessment of safflower (Carthamus tinctorius L.) collection with microsatellite markers acquired via pyrosequencing method.
abstract: A genetic evaluation of safflower germplasm collections derived from different geographical regions and countries will provide useful information for sustainable conservation and the utilization of genetic diversity. However, the molecular marker information is limited for evaluation of genetic diversity of safflower germplasm. In this study, we acquired 509 putative genomic SSR markers for sufficient genome coverage using next-generation sequencing methods and characterized thirty polymorphic SSRs in safflower collection composed of 100 diverse accessions. The average allele number and expected heterozygosity were 2.8 and 0.386, respectively. Analysis of population structure and phylogeny based on thirty SSR profiles revealed genetic admixture between geographical regions contrary to genetic clustering. However, the accessions from Korea were genetically conserved in distinctive groups in contrast to other safflower gene pool. In conclusion, these new genomic SSRs will facilitate valuable studies to clarify genetic relationships as well as conduct population structure analyses, genetic map construction and association analysis for safflower.
authors.affiliation: National Agrobiodiversity Center, National Academy of Agricultural Science, RDA, 88-20, Seodun-Dong, Suwon, Gyunggi-do, 441-707, Korea.
authors.forename: Gi-An
authors.initials: GA
authors.lastname: Lee
authors.forename: Jung-Sook
authors.initials: JS
authors.lastname: Sung
authors.forename: Sok-Young
authors.initials: SY
authors.lastname: Lee
authors.forename: Jong-Wook
authors.initials: JW
authors.lastname: Chung
authors.forename: Jung-Yoon
authors.initials: JY
authors.lastname: Yi
authors.forename: Yeon-Gyu
authors.initials: YG
authors.lastname: Kim
authors.forename: Myung-Chul
authors.initials: MC
authors.lastname: Lee
mesh_terms: D031187:Carthamus tinctorius / Q000145:classification* / Q000235:genetics*; D016000:Cluster Analysis; D018744:DNA, Plant / Q000737:chemistry / Q000235:genetics; D014644:Genetic Variation; D005828:Genetics, Population; D059014:High-Throughput Nucleotide Sequencing; D007723:Korea; D018895:Microsatellite Repeats; D008969:Molecular Sequence Data; D010802:Phylogeny; D017422:Sequence Analysis, DNA
chemical_list: D018744:DNA, Plant
keywords: Carthamus tinctorius L; Genetic diversity; SSR enrichment; Safflower; Simple sequence repeat