title: Separation and identification of 1,2,4-trihydroxynaphthalene-1-O-glucoside in Impatiens glandulifera Royle.
abstract: Methanolic extract from lyophilized roots of Impatiens glandulifera Royle was analyzed by high performance liquid chromatography using DAD and FLD detection and this revealed one dominant highly fluorescent very unstable substance. The stability of this derivative is strongly dependent on the plant material drying procedure and extraction procedure used. The structure of the substance was established as 1,2,4-trihydroxynaphthalene-1-O-glucoside (THNG) according LC-MS and NMR measurements. When lyophilized plant material was extracted with methanol an almost four times higher amount of THNG was found in the extract, compared to the amount of 2-hydroxy-1,4-naphthoquinone obtained, while in the case of the same lyophilized plant material extracted with water there was no THNG in the extract. The main compounds in this case was 2-hydroxy-1,4-naphthoquinone. In the plant material dried at the laboratory temperature and extracted by methanol there are only traces of THNG.
authors.affiliation: Laboratory of Metabolomics and Isotopic Analyses, Global Change Research Centre, Academy of Sciences of the Czech Republic, Branišovská 31, České Budějovice 37005, Czech Republic. triska.j@czechglobe.cz
authors.forename: Jan
authors.initials: J
authors.lastname: Tříska
authors.forename: Naděžda
authors.initials: N
authors.lastname: Vrchotová
authors.forename: Jan
authors.initials: J
authors.lastname: Sýkora
authors.forename: Martin
authors.initials: M
authors.lastname: Moos
mesh_terms: D002851:Chromatography, High Pressure Liquid; D005960:Glucosides / Q000737:chemistry / Q000302:isolation & purification*; D029069:Impatiens / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D013058:Mass Spectrometry; D009284:Naphthols / Q000737:chemistry* / Q000302:isolation & purification*; D010936:Plant Extracts / Q000737:chemistry*; D018517:Plant Roots / Q000737:chemistry
chemical_list: D005960:Glucosides; D009284:Naphthols; D010936:Plant Extracts