title: SNAP-25 regulates spine formation through postsynaptic binding to p140Cap.
abstract: Synaptosomal-associated protein of 25 kDa (SNAP-25) is a member of the Soluble N-ethylmaleimide-sensitive-factor attachment protein receptors (SNARE) protein family, required for exocytosis of synaptic vesicles and regulation of diverse ion channels. Here, we show that acute reduction of SNAP-25 expression leads to an immature phenotype of dendritic spines that are, consistently, less functional. Conversely, over-expression of SNAP-25 results in an increase in the density of mature, Postsynaptic Density protein 95 (PSD-95)-positive spines. The regulation of spine morphogenesis by SNAP-25 depends on the protein's ability to bind both the plasma membrane and the adaptor protein p140Cap, a key protein regulating actin cytoskeleton and spine formation. We propose that SNAP-25 allows the organization of the molecular apparatus needed for spine formation by recruiting and stabilizing p140Cap.
authors.affiliation: Department of Biotechnology and Translational Medicine, University of Milan, V Vanvitelli 32, Milano 20129, Italy.
authors.forename: Romana
authors.initials: R
authors.lastname: Tomasoni
authors.forename: Daniele
authors.initials: D
authors.lastname: Repetto
authors.forename: Raffaella
authors.initials: R
authors.lastname: Morini
authors.forename: Chiara
authors.initials: C
authors.lastname: Elia
authors.forename: Fabrizio
authors.initials: F
authors.lastname: Gardoni
authors.forename: Monica
authors.initials: M
authors.lastname: Di Luca
authors.forename: Emilia
authors.initials: E
authors.lastname: Turco
authors.forename: Paola
authors.initials: P
authors.lastname: Defilippi
authors.forename: Michela
authors.initials: M
authors.lastname: Matteoli
mesh_terms: D008841:Actin Cytoskeleton / Q000235:genetics / Q000378:metabolism; D033942:Adaptor Proteins, Vesicular Transport / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D049229:Dendritic Spines / Q000378:metabolism* / Q000648:ultrastructure; D000076122:Disks Large Homolog 4 Protein; D004622:Embryo, Mammalian; D018507:Gene Expression Regulation, Developmental; D006367:HeLa Cells; D006624:Hippocampus / Q000166:cytology / Q000196:embryology / Q000378:metabolism*; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics* / Q000378:metabolism; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D061251:Primary Cell Culture; D011485:Protein Binding; D055550:Protein Stability; D051381:Rats; D015398:Signal Transduction; D050825:Synaptosomal-Associated Protein 25 / Q000235:genetics* / Q000378:metabolism
chemical_list: D033942:Adaptor Proteins, Vesicular Transport; D000076122:Disks Large Homolog 4 Protein; C501449:Dlg4 protein, rat; D047908:Intracellular Signaling Peptides and Proteins; D008565:Membrane Proteins; C494210:Snap25 protein, rat; C000631270:Srcin1 protein, rat; D050825:Synaptosomal-Associated Protein 25