title: LC-ESI-MS method for the determination of dexamethasone acetate in skin of nude mouse.
abstract: A high-performance liquid chromatography-positive electrospray ionization single quadrupole mass spectrometric (LC-ESI-MS) method for the determination of dexamethasone acetate in skin of nude mouse using triamcinolone acetonide acetate as the internal standard (I.S.) was developed and fully validated. Both compounds were precipitated from skin homogenate with methanol and were separated by HPLC on a Shimadzu Shim-pack VP-ODS C18 column (150mm×2.0mm, 5μm) with a mobile phase of methanol-water (80:20, v/v) at a flow rate of 0.2mL/min. Calibration curves were linear over the range of 0.05-5μg/mL. The intra-run relative standard deviations were less than 9.59%. The inter-run relative standard deviations were less than 7.82%. The mean recovery was in the ranges of 89.95-95.97%, respectively. The method was successfully applied to determinate the concentration of dexamethasone acetate in skin and study the percutaneous absorption process in skin of nude mouse.
authors.affiliation: Institute of Dermatology, Chinese Academy of Medical Sciences & Peking Union Medical College, Nanjing 210042, China.
authors.forename: Lingjun
authors.initials: L
authors.lastname: Li
authors.forename: Pengcheng
authors.initials: P
authors.lastname: Ma
authors.forename: Jun
authors.initials: J
authors.lastname: Wei
authors.forename: Kun
authors.initials: K
authors.lastname: Qian
authors.forename: Lei
authors.initials: L
authors.lastname: Tao
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003907:Dexamethasone / Q000031:analogs & derivatives* / Q000032:analysis; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D012867:Skin / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
chemical_list: D003907:Dexamethasone; C018038:dexamethasone acetate
keywords: Determination; Dexamethasone acetate; LC–MS; Nude mouse; Skin