title: Development of a novel evaluation method for air particles using surface plasmon resonance spectroscopy analysis.
abstract: The aim of this study was to develop a novel evaluation method for air particles using surface plasmon resonance spectroscopy (SPR) analysis. An L1 sensor chip modified with immobilized liposome was used as a model of the membrane of epithelial cells in organs of respiration. A test suspension of dispersed air particles was flowed onto the sensor chip. The interaction between the surface of the sensor chip and particulates in the sample solution was detected by SPR. It is deduced that the SPR measurement provides information about the adsorption/desorption behavior of the particles on the membrane. Environmentally certified reference materials, diesel particulate matter, vehicle exhaust particulates, urban particulate matter, coal fly ash, and rocks, were used as air particulate samples. Filtrates of suspensions of these samples were analyzed by SPR. Each sample revealed characteristic SPR sensor-gram patterns. For example, diesel particulate matter strongly interacted with the lipid bilayer, and was hardly dissociated. On the other hand, coal fly ash and rock particles interacted poorly with the membrane. The presented method could be used to evaluate or characterize air particles.
authors.affiliation: Division of Applied Biology, Faculty of Textile Science and Technology, Shinshu University, 3-15-1 Tokida, Ueda, Nagano 386-8567, Japan.
authors.forename: Ryoya
authors.initials: R
authors.lastname: Tanaka
authors.forename: Ryusaku
authors.initials: R
authors.lastname: Gomi
authors.forename: Kunihiro
authors.initials: K
authors.lastname: Funasaka
authors.forename: Daichi
authors.initials: D
authors.lastname: Asakawa
authors.forename: Hiromitsu
authors.initials: H
authors.lastname: Nakanishi
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Moriwaki
mesh_terms: D008081:Liposomes / Q000737:chemistry; D052638:Particulate Matter / Q000032:analysis* / Q000737:chemistry; D013057:Spectrum Analysis; D020349:Surface Plasmon Resonance / Q000379:methods*
chemical_list: D008081:Liposomes; D052638:Particulate Matter