title: Blockade of IL-1R signaling diminishes Paneth cell depletion and Toxoplasma gondii induced ileitis in mice.
abstract: Interleukin 1 is a critical inflammatory mediator and involved in host defense to several pathogens. Oral T. gondii infection causes lethal ileitis in C57BL/6 (BL6) mice and serves to investigate the mechanisms of acute intestinal inflammation. Here we show that IL-1 is expressed upon oral T. gondii (76K strain) infection in the small intestine and mediates ileitis as IL-1R1 deficient mice have reduced neutrophil recruitment in the lamina propria, parasite invasion, inflammatory lesions and enhanced survival as compared to BL6 infected control mice. Protection in the absence of IL-1R1 signaling was associated with reduced IFN-γ expression and preserved Paneth cells, while these cells were eliminated in infected BL6 mice. Furthermore, blockade of IL-1 by IL-1β antibody attenuated inflammation in BL6 mice. In conclusion, IL-1 signaling contributes to the inflammatory response with increase IFN-γ expression and Paneth cell depletion upon oral T. gondii infection.
authors.affiliation: UMR 7355 Université-CNRS INEM Orléans, France.
authors.forename: Bérengère
authors.initials: B
authors.lastname: Villeret
authors.forename: Léa
authors.initials: L
authors.lastname: Brault
authors.forename: Aurélie
authors.initials: A
authors.lastname: Couturier-Maillard
authors.forename: Pauline
authors.initials: P
authors.lastname: Robinet
authors.forename: Virginie
authors.initials: V
authors.lastname: Vasseur
authors.forename: Thomas
authors.initials: T
authors.lastname: Secher
authors.forename: Isabelle
authors.initials: I
authors.lastname: Dimier-Poisson
authors.forename: Muazzam
authors.initials: M
authors.lastname: Jacobs
authors.forename: Song-Guo
authors.initials: SG
authors.lastname: Zheng
authors.forename: Valérie F
authors.initials: VF
authors.lastname: Quesniaux
authors.forename: Bernhard
authors.initials: B
authors.lastname: Ryffel
keywords: IL-1R1 receptor signaling; Paneth neutralizing antibody; Toxoplasma gondii; inflammation; innate immunity