title: Preparation and characterization of self-assembled chitosan nanoparticles for the sustained delivery of streptokinase: an in vivo study.
abstract: Chitosan (CS) nanoparticles have been extensively studied as carriers for therapeutic proteins in recent years. In this study, streptokinase loaded-CS nanoparticles were prepared and the pharmacokinetic parameters of streptokinase were compared with those of naked streptokinase. The preparation method included stirring the protein with the CS solution. The optimized combination was used for animal experiments to determine the streptokinase activity in rat plasma. Blood samples were collected at specified intervals and the activity assay was performed based on amidolysis activity of the chromogenic substrate, S2251, by streptokinase-plasminogen activator complex. The results demonstrated that streptokinase-loaded CS nanoparticles have more prolonged amidolytic activity in vivo compared to the naked one.
authors.affiliation: Department of Biology, Faculty of Basic Sciences, Kharazmi University , Tehran , Iran .
authors.forename: Seyed Mohamad Sadegh
authors.initials: SM
authors.lastname: Modaresi
authors.forename: Shahram
authors.initials: S
authors.lastname: Ejtemaei Mehr
authors.forename: Mohammad Ali
authors.initials: MA
authors.lastname: Faramarzi
authors.forename: Elina
authors.initials: E
authors.lastname: Esmaeilzadeh Gharehdaghi
authors.forename: Mahnaz
authors.initials: M
authors.lastname: Azarnia
authors.forename: Mohammad Hossein
authors.initials: MH
authors.lastname: Modarressi
authors.forename: Hadi
authors.initials: H
authors.lastname: Baharifar
authors.forename: Seyed Javad
authors.initials: SJ
authors.lastname: Vaez
authors.forename: Amir
authors.initials: A
authors.lastname: Amani
mesh_terms: D000818:Animals; D048271:Chitosan / Q000737:chemistry*; D003692:Delayed-Action Preparations / Q000737:chemistry*; D005343:Fibrinolytic Agents / Q000008:administration & dosage* / Q000097:blood; D008297:Male; D053758:Nanoparticles / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar; D055672:Static Electricity; D013300:Streptokinase / Q000008:administration & dosage* / Q000097:blood
chemical_list: D003692:Delayed-Action Preparations; D005343:Fibrinolytic Agents; D048271:Chitosan; D013300:Streptokinase