title: Differential modulation of phenotypic composition of HIV-infected and -uninfected PBMCs during cryopreservation.
abstract: This article was designed to determine variations in phenotypic composition of fresh and frozen PBMCs for assessing utility of cryopreserved PBMCs for phenotypic assays. Relative percentages of effector memory cells increased significantly as against percentages of naïve cells which showed significant decrease after cryopreservation in HIV-uninfected samples. These differences were not significant in HIV-infected individuals. There was no significant difference in the expression of activation markers in fresh and frozen PBMCs except the HLA DR expression on CD8 cells in HIV-infected individuals, which was significantly decreased in frozen PBMCs. Thus, cryopreservation resulted in differential effect on phenotypic composition of PBMCs in HIV-infected and -uninfected individuals.
authors.affiliation: National AIDS Research Institute, Bhosari, Pune, India. ashete@nariindia.org
authors.forename: Ashwini
authors.initials: A
authors.lastname: Shete
authors.forename: Priyanka
authors.initials: P
authors.lastname: Jayawant
authors.forename: Madhuri
authors.initials: M
authors.lastname: Thakar
authors.forename: Swarali
authors.initials: S
authors.lastname: Kurle
authors.forename: Dharmesh P
authors.initials: DP
authors.lastname: Singh
authors.forename: R S
authors.initials: RS
authors.lastname: Paranjape
mesh_terms: D000328:Adult; D015703:Antigens, CD / Q000276:immunology; D000945:Antigens, Differentiation, T-Lymphocyte / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D015925:Cryopreservation / Q000379:methods; D005260:Female; D015658:HIV Infections / Q000276:immunology*; D006684:HLA-DR Antigens / Q000096:biosynthesis; D006801:Humans; D019041:L-Selectin / Q000276:immunology; D037181:Lectins, C-Type / Q000276:immunology; D017493:Leukocyte Common Antigens / Q000276:immunology; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000276:immunology* / Q000378:metabolism*; D008297:Male; D010641:Phenotype; D054400:Receptors, CCR7 / Q000276:immunology; D000099192:CD69 Antigens
chemical_list: D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D006684:HLA-DR Antigens; D019041:L-Selectin; D037181:Lectins, C-Type; D017493:Leukocyte Common Antigens; D054400:Receptors, CCR7; D000099192:CD69 Antigens; C515370:CCR7 protein, human