title: CMCTS stabilized Fe3O4 particles with extremely low toxicity as highly efficient near-infrared photothermal agents for in vivo tumor ablation.
abstract: With the potential uses of photothermal therapy (PTT) in cancer treatment with excellent efficacy, and the growing concerns about the nanotoxicity of hyperthermia agents such as carbon nanotubes and gold-based nanomaterials, the importance of searching for a biocompatible hyperthermia agent cannot be emphasized too much. In this work, a novel promising hyperthermia agent employing magnetic Fe3O4 particles with fairly low toxicity was proposed. This hyperthermia agent showed rapid heat generation under NIR irradiation. After modification with carboxymethyl chitosan (CMCTS), the obtained Fe3O4@CMCTS particles could disperse stably in PBS and serum without any aggregation. The modification of CMCTS could decrease the adsorption of bovine serum albumin (BSA) and improve the cellular uptake. In a comparative study with hollow gold nanospheres (HAuNS), Fe3O4@CMCTS particles exhibited a comparable photothermal effect and fairly low cytotoxicity. The in vivo magnetic resonance (MR) images of mice revealed that by attaching a magnet to the tumor, Fe3O4@CMCTS particles accumulated in the tumor after intravenous injection and showed a low distribution in the liver. After being exposed to a 808 nm laser for 5 min at a low power density of 1.5 W cm(-2), the tumors on Fe3O4@CMCTS-injected mice reached a temperature of ~52 °C and were completely destroyed. Thus, a kind of multifunctional magnetic nanoparticle with extremely low toxicity and a simple structure for simultaneous MR imaging, targeted drug delivery and photothermal therapy can be easily fabricated.
authors.affiliation: College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China.
authors.forename: Song
authors.initials: S
authors.lastname: Shen
authors.forename: Fenfen
authors.initials: F
authors.lastname: Kong
authors.forename: Xiaomeng
authors.initials: X
authors.lastname: Guo
authors.forename: Lin
authors.initials: L
authors.lastname: Wu
authors.forename: Haijun
authors.initials: H
authors.lastname: Shen
authors.forename: Meng
authors.initials: M
authors.lastname: Xie
authors.forename: Xinshi
authors.initials: X
authors.lastname: Wang
authors.forename: Yi
authors.initials: Y
authors.lastname: Jin
authors.forename: Yanru
authors.initials: Y
authors.lastname: Ge
mesh_terms: D000818:Animals; D002417:Cattle; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D048271:Chitosan / Q000031:analogs & derivatives* / Q000737:chemistry; D004337:Drug Carriers / Q000737:chemistry; D052203:Ferrosoferric Oxide / Q000737:chemistry*; D006046:Gold / Q000737:chemistry; D006801:Humans; D007259:Infrared Rays; D061986:MCF-7 Cells; D008279:Magnetic Resonance Imaging; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D051379:Mice; D008807:Mice, Inbred BALB C; D054118:Nanospheres / Q000737:chemistry* / Q000378:metabolism / Q000633:toxicity; D009369:Neoplasms / Q000175:diagnosis / Q000188:drug therapy*; D017319:Photosensitizing Agents / Q000138:chemical synthesis / Q000627:therapeutic use*; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000378:metabolism; D013696:Temperature; D014018:Tissue Distribution
chemical_list: D004337:Drug Carriers; D058185:Magnetite Nanoparticles; D017319:Photosensitizing Agents; C514968:carboxymethyl-chitosan; D012710:Serum Albumin, Bovine; D006046:Gold; D048271:Chitosan; D052203:Ferrosoferric Oxide