title: Amphiphilic oligoethyleneimine-β-cyclodextrin "click" clusters for enhanced DNA delivery.
abstract: Monodisperse amphiphilic oligoethyleneimine (OEI)-β-cyclodextrin (βCD) clusters have been prepared, and their potential as gene delivery systems has been evaluated in comparison with a nonamphiphilic congener. The general prototype incorporates tetraethyleneimine segments linked to the primary rim of βCD through either triazolyl or thioureidocysteaminyl connectors. Transfection efficiency data for the corresponding CD:pDNA nanocomplexes (CDplexes) in BNL-CL2 murine hepatocytes evidenced the strong beneficial effect of facial amphiphilicity.
authors.affiliation: Departamento de Química Orgánica, Facultad de Química, Universidad de Sevilla, Profesor García González 1, E-41012 Sevilla, Spain.
authors.forename: Álvaro
authors.initials: Á
authors.lastname: Martínez
authors.forename: Céline
authors.initials: C
authors.lastname: Bienvenu
authors.forename: José L
authors.initials: JL
authors.lastname: Jiménez Blanco
authors.forename: Pierre
authors.initials: P
authors.lastname: Vierling
authors.forename: Carmen Ortiz
authors.initials: CO
authors.lastname: Mellet
authors.forename: José M
authors.initials: JM
authors.lastname: García Fernández
authors.forename: Christophe
authors.initials: C
authors.lastname: Di Giorgio
mesh_terms: D000818:Animals; D001388:Aziridines / Q000737:chemistry*; D002460:Cell Line; D057930:Click Chemistry; D004247:DNA / Q000737:chemistry*; D022781:Hepatocytes / Q000737:chemistry; D051379:Mice; D015394:Molecular Structure; D049329:Nanostructures / Q000737:chemistry*; D010957:Plasmids; D013501:Surface-Active Agents / Q000138:chemical synthesis / Q000737:chemistry*; D047392:beta-Cyclodextrins / Q000737:chemistry*
chemical_list: D001388:Aziridines; D013501:Surface-Active Agents; D047392:beta-Cyclodextrins; C033132:aziridine; D004247:DNA; C031215:betadex