title: Emergence and spread of carbapenem-resistant Acinetobacter baumannii sequence type 191 in a Korean hospital.
abstract: Emergence and spread of specific carbapenem-resistant Acinetobacter baumannii (CRAB) clones cause a serious therapeutic problem. This study was aimed to investigate the clonal diversity and genetic basis of antimicrobial resistance among the 69 CRAB isolates from 2009 to 2010 in a Korean hospital. All CRAB isolates were found to be sequence type (ST) 2 using the Institute Pasteur's multilocus sequence typing (MLST) scheme, but classified into two sequence groups and nine pulsotypes. Fifty-six CRAB isolates belonging to two main pulsotypes were found to be ST191 using the Bartual's MLST scheme. All CRAB isolates showed an extensively drug-resistant phenotype. The blaOXA-51/blaOXA-23, blaAmpC/blaPER-1 and armA genes were largely responsible for resistance to carbapenems, extended-spectrum β-lactams and aminoglycosides, respectively. The first CRAB strains identified in 2005 in this hospital were found to be ST2 using the Institute Pasteur's MLST scheme, but showed ST353 using the Bartual's MLST scheme and different pulsotypes from the CRAB isolates from 2009 to 2010. In conclusion, this is the first report of emergence and spread of A. baumannii ST191 in Korea, as well of the genetic basis of its antimicrobial resistance.
authors.affiliation: Department of Microbiology, Kyungpook National University School of Medicine, Daegu 700-422, South Korea.
authors.forename: Mamata
authors.initials: M
authors.lastname: Gurung
authors.forename: Jae Su
authors.initials: JS
authors.lastname: Rho
authors.forename: Yoo Chul
authors.initials: YC
authors.lastname: Lee
authors.forename: Hyung Sun
authors.initials: HS
authors.lastname: Kim
authors.forename: Sun Young
authors.initials: SY
authors.lastname: Moon
authors.forename: Byung Hyuk
authors.initials: BH
authors.lastname: Yu
authors.forename: Joo Hyung
authors.initials: JH
authors.lastname: Lee
authors.forename: Cheol Woo
authors.initials: CW
authors.lastname: Jang
authors.forename: Seung Yeon
authors.initials: SY
authors.lastname: Chung
authors.forename: Il Hwan
authors.initials: IH
authors.lastname: Cho
authors.forename: Hyeon Sook
authors.initials: HS
authors.lastname: Jee
authors.forename: Seung Won
authors.initials: SW
authors.lastname: Choi
authors.forename: Ji Young
authors.initials: JY
authors.lastname: Heo
authors.forename: Je Chul
authors.initials: JC
authors.lastname: Lee
mesh_terms: D000151:Acinetobacter Infections / Q000382:microbiology*; D040981:Acinetobacter baumannii / Q000187:drug effects* / Q000235:genetics*; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D015780:Carbapenems / Q000494:pharmacology*; D016000:Cluster Analysis; D003428:Cross Infection / Q000382:microbiology*; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D024901:Drug Resistance, Multiple, Bacterial; D006785:Hospitals, University; D006801:Humans; D056910:Republic of Korea; D018440:beta-Lactam Resistance
chemical_list: D000900:Anti-Bacterial Agents; D015780:Carbapenems; D004269:DNA, Bacterial
keywords: Carbapenem; Clonal spread; Extended-spectrum β-lactamase; Multi-drug resistance; Sequence type