title: Acceleration of peripheral nerve regeneration through asymmetrically porous nerve guide conduit applied with biological/physical stimulation.
abstract: Sufficient functional restoration of damaged peripheral nerves is a big clinical challenge. In this study, a nerve guide conduit (NGC) with selective permeability was prepared by rolling an asymmetrically porous polycaprolactone/Pluronic F127 membrane fabricated using a novel immersion precipitation method. Dual stimulation (nerve growth factor [NGF] as a biological stimulus and low-intensity pulse ultrasound [US] as a physical stimulus) was adapted to enhance nerve regeneration through an NGC. The animal study revealed that each stimulation (NGF or US) has a positive effect to promote the peripheral nerve regeneration through the NGC, however, the US-stimulated NGC group allowed more accelerated nerve regeneration compared with the NGF-stimulated group. The NGC group that received dual stimulation (NGF and US) showed more effective nerve regeneration behavior than the groups that received a single stimulation (NGF or US). The asymmetrically porous NGC with dual NGF and US stimulation may be a promising strategy for the clinical treatment of delayed and insufficient functional recovery of a peripheral nerve.
authors.affiliation: 1 Department of Advanced Materials, Hannam University , Daejeon, Republic of Korea.
authors.forename: Jin Rae
authors.initials: JR
authors.lastname: Kim
authors.forename: Se Heang
authors.initials: SH
authors.lastname: Oh
authors.forename: Gu Birm
authors.initials: GB
authors.lastname: Kwon
authors.forename: Uk
authors.initials: U
authors.lastname: Namgung
authors.forename: Kyu Sang
authors.initials: KS
authors.lastname: Song
authors.forename: Byeong Hwa
authors.initials: BH
authors.lastname: Jeon
authors.forename: Jin Ho
authors.initials: JH
authors.lastname: Lee
mesh_terms: D000818:Animals; D020932:Nerve Growth Factor / Q000737:chemistry*; D009416:Nerve Regeneration; D059348:Peripheral Nerve Injuries / Q000473:pathology / Q000628:therapy*; D010525:Peripheral Nerves / Q000378:metabolism* / Q000473:pathology; D020442:Poloxamer / Q000737:chemistry*; D011091:Polyesters / Q000737:chemistry*; D016062:Porosity; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D011091:Polyesters; D020442:Poloxamer; C016240:polycaprolactone; D020932:Nerve Growth Factor