title: Current concepts in the diagnosis of blood stream infections. Are novel molecular methods useful in clinical practice?
abstract: The decision on the right empirical treatment in bacteremia places particular demands on the clinician. As long as no microbiological diagnosis can be immediately drawn, the clinical diagnosis together with knowledge of local antimicrobial resistance must determine the antimicrobial choice. The use of several amplification, hybridization, and mass spectrometry methods has been studied in patient cohorts in comparison with blood culture-based conventional techniques. However, no clinical outcome trials have been conducted in which the use of these novel methods would guide antimicrobial therapy. Local differences in bacterial antimicrobial resistance cause differences in the regional need for molecular methods for the early detection of resistance mechanisms. The implementation of novel methods in clinical use requires active discussion between laboratory experts and clinicians. Providing rapid susceptibility results using conventional methods can lead to timely changes to appropriate antimicrobial therapy and the costs are lower than with the molecular methods. Gram-stain information in combination with clinical data is an underestimated, underused, rapid, and economical means of assessing the etiology of blood stream infection.
authors.affiliation: Department of Internal Medicine, Tampere University Hospital, Box 2000, FI-33521 Tampere, Finland; University of Tampere Medical School, University of Tampere, Tampere, Finland. Electronic address: Reetta.Huttunen@uta.fi.
authors.forename: Reetta
authors.initials: R
authors.lastname: Huttunen
authors.forename: Jaana
authors.initials: J
authors.lastname: Syrjänen
authors.forename: Risto
authors.initials: R
authors.lastname: Vuento
authors.forename: Janne
authors.initials: J
authors.lastname: Aittoniemi
mesh_terms: D000890:Anti-Infective Agents / Q000627:therapeutic use; D016470:Bacteremia / Q000175:diagnosis* / Q000188:drug therapy / Q000382:microbiology; D001431:Bacteriological Techniques; D006801:Humans; D008826:Microbial Sensitivity Tests; D018805:Sepsis / Q000175:diagnosis* / Q000188:drug therapy / Q000209:etiology
chemical_list: D000890:Anti-Infective Agents
keywords: Bacteremia; Blood culture; Diagnosis; Molecular; PCR; Sepsis