title: Oligodeoxynucleotides containing multiple thiophene-modified isomorphic fluorescent nucleosides.
abstract: 5-(Thien-2-yl)-2'-deoxyuridine, an isomorphic fluorescent nucleoside analogue, was incorporated into multiple positions within single stranded oligodeoxynucleotides. With minimal impact on duplex stability and overall structure, oligonucleotides containing three identical isomorphic fluorescent nucleosides in alternating or neighboring positions display enhanced, sequence-dependent on-signals for either duplex formation or dissociation.
authors.affiliation: Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla, California 92093-0358, USA.
authors.forename: Mary S
authors.initials: MS
authors.lastname: Noé
authors.forename: Renatus W
authors.initials: RW
authors.lastname: Sinkeldam
authors.forename: Yitzhak
authors.initials: Y
authors.lastname: Tor
mesh_terms: D005453:Fluorescence; D008968:Molecular Conformation; D009705:Nucleosides / Q000138:chemical synthesis* / Q000737:chemistry; D009838:Oligodeoxyribonucleotides / Q000737:chemistry*; D013876:Thiophenes / Q000737:chemistry*
chemical_list: D009705:Nucleosides; D009838:Oligodeoxyribonucleotides; D013876:Thiophenes