title: The dysfunction of CD4(+)CD25(+) regulatory T cells contributes to the abortion of mice caused by Toxoplasma gondii excreted-secreted antigens in early pregnancy.
abstract: Toxoplasma gondii is an opportunistic intracellular parasite that is highly prevalent in human and warm-blooded animals throughout the world, leading to potentially severe congenital infections. Although the abortion caused by T. gondii is believed to be dependent on the timing of maternal infection during pregnancy, the mechanism remains unclear. This study was focused on the effects of T. gondii excreted-secreted antigens on pregnant outcomes and CD4(+)CD25(+) Foxp3(+) regulatory T cells at different stages of pregnancy. The results showed that in mice the frequency and suppressive function of CD4(+)CD25(+) regulatory cells were diminished after injection of T. gondii excreted-secreted antigens at early and intermediate stages of pregnancy. The abortion caused by T. gondii excreted-secreted antigens at early pregnancy could be partly prevented by adoptively transferring of CD4(+)CD25(+) cells from the mice injected with T. gondii excreted-secreted antigens at late pregnancy, but not from the mice with the same treatment at early pregnancy. Furthermore, T. gondii excreted-secreted antigens induced apoptosis of CD4(+)CD25(+) regulatory cells of mice in early and intermediate stages of pregnancy by down-regulating their Bcl-2 expressions and Bcl-2/Bax ratio. This study provides new insights into the mechanism that T. gondii infection is the high risk factor for abortion in early pregnancy.
authors.affiliation: Department of Pathogen Biology, Key Laboratory of Pathogen Biology of Jiangsu Province, Nanjing Medical University, Nanjing, Jiangsu, China.
authors.forename: Jin-ling
authors.initials: JL
authors.lastname: Chen
authors.forename: Yi-yue
authors.initials: YY
authors.lastname: Ge
authors.forename: Jie
authors.initials: J
authors.lastname: Zhang
authors.forename: Xiao-yan
authors.initials: XY
authors.lastname: Qiu
authors.forename: Jing-fan
authors.initials: JF
authors.lastname: Qiu
authors.forename: Jiang-ping
authors.initials: JP
authors.lastname: Wu
authors.forename: Yong
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000022:Abortion, Spontaneous; D000818:Animals; D000953:Antigens, Protozoan / Q000276:immunology*; D001483:Base Sequence; D015704:CD4 Antigens / Q000276:immunology*; D017931:DNA Primers; D005260:Female; D053645:Interleukin-2 Receptor alpha Subunit / Q000276:immunology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D011247:Pregnancy; D011256:Pregnancy Outcome; D060888:Real-Time Polymerase Chain Reaction; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*; D014122:Toxoplasma / Q000276:immunology*
chemical_list: D000953:Antigens, Protozoan; D015704:CD4 Antigens; D017931:DNA Primers; D053645:Interleukin-2 Receptor alpha Subunit