title: ApoE enhances nanodisk-mediated curcumin delivery to glioblastoma multiforme cells.
abstract: OBJECTIVE
  To evaluate the effect of incorporating the polyphenol, curcumin, into nanodisk (ND) particles on its biological activity.
  
  
  METHODS
  Curcumin-NDs formulated with different scaffold proteins were incubated with cultured glioblastoma multiforme cells.
  
  
  RESULTS
  When ApoE was employed as the ND scaffold protein, enhanced curcumin uptake was observed. Furthermore, ApoE curcumin-NDs induced greater cell death than either free curcumin or ApoAI curcumin-NDs. A total of 1 h after exposure of glioblastoma multiforme cells to ApoE curcumin-NDs, significant curcumin uptake was detected while ApoE was localized at the cell surface. After 2 h, a portion of the curcumin had migrated to the nucleus, giving rise to enhanced fluorescence intensity in discrete intranuclear sites.
  
  
  CONCLUSIONS
  ApoE-mediated interaction of curcumin-NDs with glioblastoma multiforme cells leads to enhanced curcumin uptake and increased biological activity.
authors.affiliation: Children's Hospital Oakland Research Institute, 5700 Martin Luther King Jr Way, Oakland, CA 94609, USA.
authors.forename: Mistuni
authors.initials: M
authors.lastname: Ghosh
authors.forename: Robert O
authors.initials: RO
authors.lastname: Ryan
mesh_terms: D000970:Antineoplastic Agents / Q000008:administration & dosage* / Q000493:pharmacokinetics / Q000494:pharmacology; D001057:Apolipoproteins E / Q000737:chemistry* / Q000378:metabolism; D017209:Apoptosis / Q000187:drug effects; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D003474:Curcumin / Q000008:administration & dosage* / Q000493:pharmacokinetics / Q000494:pharmacology; D004337:Drug Carriers / Q000737:chemistry* / Q000378:metabolism; D005909:Glioblastoma / Q000188:drug therapy* / Q000473:pathology; D006801:Humans; D049329:Nanostructures / Q000737:chemistry*
chemical_list: D000970:Antineoplastic Agents; D001057:Apolipoproteins E; D004337:Drug Carriers; D003474:Curcumin
keywords: ApoE; apoptosis; confocal fluorescence microscopy; curcumin; glioblastoma; nanodisk