title: Short read mapping for exome sequencing.
abstract: Mapping short reads to the reference genome is very often the prerequisite for applications utilizing the next-generation sequencing technologies. A dozen of software tools developed for this purpose have been widely used. But many practical issues remained when utilizing them to build a computational pipeline for downstream analyses. In this chapter, we describe the read mapping procedures adopted in our lab for the exome sequencing studies as an example to illustrate those practical details.
authors.affiliation: Bioinformatics Division, Tsinghua National Laboratory of Information Science and Technology, Beijing, China.
authors.forename: Xueya
authors.initials: X
authors.lastname: Zhou
authors.forename: Suying
authors.initials: S
authors.lastname: Bao
authors.forename: Binbin
authors.initials: B
authors.lastname: Wang
authors.forename: Xuegong
authors.initials: X
authors.lastname: Zhang
authors.forename: You-Qiang
authors.initials: YQ
authors.lastname: Song
mesh_terms: D000818:Animals; D059472:Exome; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D006801:Humans; D016415:Sequence Alignment / Q000379:methods; D012984:Software