title: Conditioned medium from periapical follicle cells induces the odontogenic differentiation of stem cells from the apical papilla in vitro.
abstract: BACKGROUND
  We investigated the biological effects of conditioned medium (CM) from periapical follicle cells (PAFCs) of root-developing tooth on the proliferation and differentiation of stem cells from the apical papilla (SCAP) in vitro.
  
  
  METHODS
  Human SCAP and PAFCs were isolated and expanded. CM from PAFCs was prepared with the primary cells. Cell cycle analysis, methyl-thiazol-diphenyltetrazolium assay, alkaline phosphatase activity, mineralization behavior, and gene expression of odontoblast phenotype SCAP cultured with or without CM from PAFCs were evaluated.
  
  
  RESULTS
  In the CM-treated group, the cell growth, alkaline phosphatase activity, and mineralization of SCAP were up-regulated. The expression of dentin sialophosphoprotein, alkaline phosphatase, and osteocalcin mRNA progressively increased in SCAP treated with CM from PAFCs.
  
  
  CONCLUSIONS
  Our findings suggest that CM from PAFCs is able to provide a favorable odontogenic microenvironment to induce differentiation of SCAP along the odontoblast lineage.
authors.affiliation: Hospital of Stomatology, Zunyi Medical College, Zunyi, Guizhou, People's Republic of China.
authors.forename: Jiayuan
authors.initials: J
authors.lastname: Wu
authors.forename: Qian
authors.initials: Q
authors.lastname: Jia
authors.forename: Wenxi
authors.initials: W
authors.lastname: He
authors.forename: Jianguo
authors.initials: J
authors.lastname: Liu
authors.forename: Lipeng
authors.initials: L
authors.lastname: Hou
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.forename: Zhongying
authors.initials: Z
authors.lastname: Niu
authors.forename: Longxing
authors.initials: L
authors.lastname: Ni
mesh_terms: D000293:Adolescent; D000469:Alkaline Phosphatase / Q000032:analysis; D002113:Calcification, Physiologic / Q000502:physiology; D018929:Cell Culture Techniques; D002453:Cell Cycle / Q000502:physiology; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology; D019070:Cell Lineage; D002470:Cell Survival / Q000502:physiology; D002478:Cells, Cultured; D060833:Cellular Microenvironment / Q000502:physiology; D017077:Culture Media, Conditioned / Q000494:pharmacology*; D003771:Dental Papilla / Q000166:cytology* / Q000187:drug effects; D003795:Dental Sac / Q000166:cytology* / Q000187:drug effects; D016326:Extracellular Matrix Proteins / Q000032:analysis; D006801:Humans; D009804:Odontoblasts / Q000187:drug effects / Q000502:physiology; D009805:Odontogenesis / Q000502:physiology*; D015675:Osteocalcin / Q000032:analysis; D010486:Periapical Tissue / Q000166:cytology* / Q000187:drug effects; D010641:Phenotype; D010750:Phosphoproteins / Q000032:analysis; D012795:Sialoglycoproteins / Q000032:analysis; D013234:Stem Cells / Q000502:physiology*; D019227:Tooth Apex / Q000166:cytology* / Q000187:drug effects; D015854:Up-Regulation; D000099311:Dentin Sialophosphoprotein
chemical_list: D000469:Alkaline Phosphatase; D017077:Culture Media, Conditioned; D016326:Extracellular Matrix Proteins; D015675:Osteocalcin; D010750:Phosphoproteins; D012795:Sialoglycoproteins; D000099311:Dentin Sialophosphoprotein
keywords: Conditioned medium; microenvironment; periapical follicle cells; stem cells of the apical papilla