title: Kinesin-5/Eg5 is important for transport of CARTS from the trans-Golgi network to the cell surface.
abstract: Here we report that the kinesin-5 motor Klp61F, which is known for its role in bipolar spindle formation in mitosis, is required for protein transport from the Golgi complex to the cell surface in Drosophila S2 cells. Disrupting the function of its mammalian orthologue, Eg5, in HeLa cells inhibited secretion of a protein called pancreatic adenocarcinoma up-regulated factor (PAUF) but, surprisingly, not the trafficking of vesicular stomatitis virus G protein (VSV-G) to the cell surface. We have previously reported that PAUF is transported from the trans-Golgi network (TGN) to the cell surface in specific carriers called CARTS that exclude VSV-G. Inhibition of Eg5 function did not affect the biogenesis of CARTS; however, their migration was delayed and they accumulated near the Golgi complex. Altogether, our findings reveal a surprising new role of Eg5 in nonmitotic cells in the facilitation of the transport of specific carriers, CARTS, from the TGN to the cell surface.
authors.affiliation: Cell and Developmental Biology Programme, Centre for Genomic Regulation (CRG), 08003 Barcelona, Spain.
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Wakana
authors.forename: Julien
authors.initials: J
authors.lastname: Villeneuve
authors.forename: Josse
authors.initials: J
authors.lastname: van Galen
authors.forename: David
authors.initials: D
authors.lastname: Cruz-Garcia
authors.forename: Mitsuo
authors.initials: M
authors.lastname: Tagaya
authors.forename: Vivek
authors.initials: V
authors.lastname: Malhotra
mesh_terms: D000818:Animals; D051784:Aryl Hydrocarbon Receptor Nuclear Translocator / Q000235:genetics / Q000378:metabolism; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D004330:Drosophila / Q000235:genetics / Q000378:metabolism; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D004721:Endoplasmic Reticulum / Q000378:metabolism; D006056:Golgi Apparatus / Q000235:genetics / Q000378:metabolism*; D057809:HEK293 Cells; D006367:HeLa Cells; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins; D016547:Kinesins / Q000235:genetics / Q000378:metabolism*; D037102:Lectins / Q000378:metabolism; D008562:Membrane Glycoproteins / Q000235:genetics / Q000378:metabolism*; D008869:Microtubule-Associated Proteins / Q000235:genetics / Q000378:metabolism*; D008870:Microtubules / Q000378:metabolism; D021381:Protein Transport; D014162:Transfection; D014759:Viral Envelope Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D002352:Carrier Proteins; D029721:Drosophila Proteins; C028391:G protein, vesicular stomatitis virus; D036341:Intercellular Signaling Peptides and Proteins; C470243:KIF11 protein, human; C084034:Klp61F protein, Drosophila; D037102:Lectins; D008562:Membrane Glycoproteins; D008869:Microtubule-Associated Proteins; D014759:Viral Envelope Proteins; C517340:ZG16B protein, human; C109766:tgo protein, Drosophila; D051784:Aryl Hydrocarbon Receptor Nuclear Translocator; D016547:Kinesins