title: Regulation of lipid and glucose homeostasis by mango tree leaf extract is mediated by AMPK and PI3K/AKT signaling pathways.
abstract: Ethanolic extract of Mangifera indica (mango) dose-dependently decreased serum glucose and triglyceride in KK-A(y) mice. Our in vitro and in vivo investigations revealed that the effect of mango leave extract (ME) on glucose and lipid homeostasis is mediated, at least in part, through the PI3K/AKT and AMPK signaling pathway. ME up-regulated the expression of PI3K, AKT and GYS genes by 2.0-fold, 3.2-fold, and 2.7-fold, respectively, leading to a decrease in glucose level. On the other hand, ME up-regulated AMPK and altered lipid metabolism. ME also down-regulated ACC (2.8-fold), HSL (1.6-fold), FAS (1.8-fold) and PPAR-γ (4.0-fold). Finally, we determined that active metabolites of benzophenone C-glucosides, Iriflophenone 3-C-β-glucoside and Foliamangiferoside A from ME, may play a dominant role in this integrated regulation of sugar and lipid homeostasis.
authors.affiliation: Tianjin State Key Laboratory of Modern Chinese Medicine, 312 Anshanxi Road, Nankai District, Tianjin 300193, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhang
authors.forename: Xuefeng
authors.initials: X
authors.lastname: Liu
authors.forename: Lifeng
authors.initials: L
authors.lastname: Han
authors.forename: Xiumei
authors.initials: X
authors.lastname: Gao
authors.forename: Erwei
authors.initials: E
authors.lastname: Liu
authors.forename: Tao
authors.initials: T
authors.lastname: Wang
mesh_terms: D041721:3T3-L1 Cells; D055372:AMP-Activated Protein Kinases / Q000235:genetics / Q000378:metabolism; D000818:Animals; D005260:Female; D005947:Glucose / Q000378:metabolism*; D044882:Glucose Metabolism Disorders / Q000188:drug therapy* / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D006706:Homeostasis / Q000187:drug effects*; D006801:Humans; D050356:Lipid Metabolism / Q000187:drug effects*; D008297:Male; D031022:Mangifera / Q000737:chemistry*; D051379:Mice; D008810:Mice, Inbred C57BL; D051058:Oncogene Protein v-akt / Q000235:genetics / Q000378:metabolism; D047495:PPAR gamma / Q000235:genetics / Q000378:metabolism; D019869:Phosphatidylinositol 3-Kinases / Q000235:genetics / Q000378:metabolism; D010936:Plant Extracts / Q000008:administration & dosage* / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry; D015398:Signal Transduction / Q000187:drug effects*; D014197:Trees / Q000737:chemistry; D014280:Triglycerides / Q000378:metabolism
chemical_list: D047495:PPAR gamma; D010936:Plant Extracts; D014280:Triglycerides; D019869:Phosphatidylinositol 3-Kinases; D051058:Oncogene Protein v-akt; D055372:AMP-Activated Protein Kinases; D005947:Glucose
keywords: 3T3-L1 cells; AMPK signaling pathway; Glucose and lipid regulation; Mangifera indica; PI3K/AKT signaling pathway