title: Changes of inhibitory receptors on NK-92 cells and HLA-G on BeWo cells with Toxoplasma gondii infection.
abstract: The aim of this study were to demonstrate the effects of Yellow fluorescent protein-Toxoplasma gondii infection on the NK-92 cells co-cultured with BeWo cells in vitro and to verify the implications upon adverse pregnancy outcome. There are four groups including NK-92 cells infected or uninfected with T. gondii, and NK-92 cells co-cultured with BeWo cells in the presence or absent of T. gondii infection. Cells were observed by fluorescence microscope at 12, 24, 36, 48, and 60 h after infection. Levels of inhibitory receptors killer immunoglobulin-like receptor (KIR)2DL4 and ILT-2 expressed on NK-92 cells, and their common ligand HLA-G expressed on BeWo cells were measured by real-time PCR and flow cytometry in all groups. Levels of KIR2DL4, ILT-2 and HLA-G increased at 12, 24, 36, and 48 h, while decreased at 60 h in all T. gondii-infected groups compared with their parallel control groups, respectively. KIR2DL4 and ILT-2 expression in co-culture groups with infection were higher than those in infected NK-92 groups at 12, 24, 36, and 48 h, while there were no significant differences at 60 h. The expression of the two inhibitory receptors was correlated with HLA-G expression in infected co-culture groups. The changes of inhibitory receptors on NK-92 cells and their common ligand HLA-G on BeWo cells with T. gondii infection might contribute to the occurrence of adverse pregnancy outcome.
authors.affiliation: Department of Immunology, Binzhou Medical University, No.346 Guan-Hai Road, Lai-shan, Yantai, Shandong, 264003, People's Republic of China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.forename: Ling
authors.initials: L
authors.lastname: Zhang
authors.forename: Meilan
authors.initials: M
authors.lastname: Gao
authors.forename: Fengmei
authors.initials: F
authors.lastname: Zhang
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Xu
authors.forename: Xianbing
authors.initials: X
authors.lastname: Liu
authors.forename: Xuemei
authors.initials: X
authors.lastname: Hu
mesh_terms: D015703:Antigens, CD / Q000378:metabolism; D002460:Cell Line; D018920:Coculture Techniques; D005260:Female; D059951:HLA-G Antigens / Q000378:metabolism; D006801:Humans; D007694:Killer Cells, Natural / Q000276:immunology*; D000075362:Leukocyte Immunoglobulin-like Receptor B1; D011247:Pregnancy; D011248:Pregnancy Complications / Q000276:immunology / Q000469:parasitology*; D011971:Receptors, Immunologic / Q000378:metabolism; D054345:Receptors, KIR2DL4 / Q000378:metabolism; D014122:Toxoplasma / Q000276:immunology*; D014123:Toxoplasmosis / Q000276:immunology*
chemical_list: D015703:Antigens, CD; D059951:HLA-G Antigens; C515079:KIR2DL4 protein, human; C105299:LILRB1 protein, human; D000075362:Leukocyte Immunoglobulin-like Receptor B1; D011971:Receptors, Immunologic; D054345:Receptors, KIR2DL4