title: Neonatal immunization with respiratory syncytial virus glycoprotein fragment induces protective immunity in the presence of maternal antibodies in mice.
abstract: Respiratory syncytial virus (RSV) is a major cause of severe lower respiratory tract infections in infants and the elderly worldwide. The significant morbidity and mortality associated with this infection underscores the urgent need for development of RSV vaccine. In this study, we first show that intranasal administration of RSV glycoprotein core fragment (Gcf) to neonatal mice can induce systemic humoral immune responses and protective immunity against RSV without causing lung eosinophilia, although antibody response was shifted to a Th2 response. Next, we examined whether the presence of maternal anti-RSV antibodies would affect the responsiveness and protection efficacy of Gcf in newborn mice, since infants can possess RSV-specific maternal antibodies due to frequent RSV re-infections to adults. Intranasal administration of Gcf induced antibody response and increased IFNγ secretion and protected mice against RSV challenge without severe lung eosinophilia, even in the presence of high levels of RSV-specific maternal antibodies. Thus, our findings suggest that Gcf may be an effective and safe RSV vaccine during the neonatal period.
authors.affiliation: Laboratory Science Division , International Vaccine Institute, Seoul, Korea.
authors.forename: Youran
authors.initials: Y
authors.lastname: Noh
authors.forename: Byoung-Shik
authors.initials: BS
authors.lastname: Shim
authors.forename: In Su
authors.initials: IS
authors.lastname: Cheon
authors.forename: Semi
authors.initials: S
authors.lastname: Rho
authors.forename: Hee Joo
authors.initials: HJ
authors.lastname: Kim
authors.forename: Youngjoo
authors.initials: Y
authors.lastname: Choi
authors.forename: Chang-Yuil
authors.initials: CY
authors.lastname: Kang
authors.forename: Jun
authors.initials: J
authors.lastname: Chang
authors.forename: Man Ki
authors.initials: MK
authors.lastname: Song
authors.forename: Jae-Ouk
authors.initials: JO
authors.lastname: Kim
mesh_terms: D000281:Administration, Intranasal; D000818:Animals; D000831:Animals, Newborn / Q000276:immunology; D000914:Antibodies, Viral / Q000276:immunology; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology; D002460:Cell Line; D004802:Eosinophilia / Q000276:immunology; D005260:Female; D006023:Glycoproteins / Q000008:administration & dosage / Q000276:immunology*; D007114:Immunization / Q000379:methods; D007371:Interferon-gamma / Q000096:biosynthesis / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D018357:Respiratory Syncytial Virus Infections / Q000276:immunology* / Q000517:prevention & control / Q000821:virology; D022261:Respiratory Syncytial Virus Vaccines / Q000008:administration & dosage / Q000276:immunology*; D012136:Respiratory Syncytial Viruses / Q000276:immunology*; D012141:Respiratory Tract Infections / Q000276:immunology* / Q000517:prevention & control / Q000821:virology
chemical_list: D000914:Antibodies, Viral; D006023:Glycoproteins; D022261:Respiratory Syncytial Virus Vaccines; D007371:Interferon-gamma