title: Cytochrome P450 1A1, 2E1 and GSTM1 gene polymorphisms and susceptibility to colorectal cancer in the Saudi population.
abstract: BACKGROUND
  The Saudi population has experienced a sharp increase in colorectal and gastric cancer incidences within the last few years. The relationship between gene polymorphisms of xenobiotic metabolizing enzymes and colorectal cancer (CRC) incidence has not previously investigated among the Saudi population. The aim of the present study was to investigate contributions of CYP1A1, CYP2E1, and GSTM1 gene polymorphisms.
  
  
  METHODS
  Blood samples were collected from CRC patients and healthy controls and genotypes were determined by polymerase chain reaction restriction fragment length polymorphism and sequencing.
  
  
  CONCLUSIONS
  CYP2E1*6 was not significantly associated with CRC development (odd ratio=1.29; confidence interval 0.68-2.45). A remarkable and statistically significant association was observed among patients with CYP1Awt/*2A (odd ratio=3.65; 95% confidence interval 1.39-9.57). The GSTM1*0/*0 genotype was found in 2% of CRC patients under investigation. The levels of CYP1A1, CYP2E1 and GSTM1 mRNA gene expression were found to be 4, 4.2 and 4.8 fold, respectively, by quantitative real time PCR. The results of the present case-control study show that the studied Saudi population resembles Caucasians with respect to the considered polymorphisms. Investigation of genetic risk factors and susceptibility gene polymorphisms in our Saudi population should be helpful for better understanding of CRC etiology.
authors.affiliation: Department of Biochemistry, College of Science, King Saud University, Riyadh, KSA. hesham25166@hotmail.com
authors.forename: Hesham Mahmoud
authors.initials: HM
authors.lastname: Saeed
authors.forename: Mohammad Saud
authors.initials: MS
authors.lastname: Alanazi
authors.forename: Howaida Attia
authors.initials: HA
authors.lastname: Nounou
authors.forename: Manal Ali
authors.initials: MA
authors.lastname: Salaby
authors.forename: Abdelhabib
authors.initials: A
authors.lastname: Semlali
authors.forename: Nahla
authors.initials: N
authors.lastname: Azzam
authors.forename: Abdeulrahan
authors.initials: A
authors.lastname: Aljebreen
authors.forename: Othman
authors.initials: O
authors.lastname: Alharby
authors.forename: Narasimha Reddy
authors.initials: NR
authors.lastname: Parine
authors.forename: Jilani
authors.initials: J
authors.lastname: Shaik
authors.forename: Maha
authors.initials: M
authors.lastname: Arafaha
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D016022:Case-Control Studies; D003106:Colon / Q000378:metabolism; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D019363:Cytochrome P-450 CYP1A1 / Q000235:genetics* / Q000378:metabolism; D019392:Cytochrome P-450 CYP2E1 / Q000235:genetics* / Q000378:metabolism; D005260:Female; D005500:Follow-Up Studies; D020022:Genetic Predisposition to Disease; D005838:Genotype; D005982:Glutathione Transferase / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D011379:Prognosis; D012007:Rectum / Q000378:metabolism; D012529:Saudi Arabia; D015996:Survival Rate; D014407:Tumor Cells, Cultured
chemical_list: D019363:Cytochrome P-450 CYP1A1; D019392:Cytochrome P-450 CYP2E1; D005982:Glutathione Transferase