title: Divergent RNA transcription: a role in promoter unwinding?
abstract: New approaches using biotinylated-psoralen as a probe for investigating DNA structure have revealed new insights into the relationship between DNA supercoiling, transcription and chromatin compaction. We explore a hypothesis that divergent RNA transcription generates negative supercoiling at promoters facilitating initiation complex formation and subsequent promoter clearance.
authors.affiliation: MRC Human Genetics Unit; Institute of Genetics and Molecular Medicine; The University of Edinburgh; Edinburgh, UK.
authors.forename: Catherine
authors.initials: C
authors.lastname: Naughton
authors.forename: Samuel
authors.initials: S
authors.lastname: Corless
authors.forename: Nick
authors.initials: N
authors.lastname: Gilbert
mesh_terms: D002843:Chromatin / Q000235:genetics / Q000378:metabolism; D004278:DNA, Superhelical / Q000737:chemistry / Q000378:metabolism; D006657:Histones / Q000378:metabolism; D006801:Humans; D011401:Promoter Regions, Genetic; D012313:RNA / Q000737:chemistry / Q000378:metabolism*; D012319:RNA Polymerase II / Q000378:metabolism; D016372:RNA, Antisense / Q000378:metabolism; D014158:Transcription, Genetic
chemical_list: D002843:Chromatin; D004278:DNA, Superhelical; D006657:Histones; D016372:RNA, Antisense; D012313:RNA; D012319:RNA Polymerase II
keywords: DNA; anti-sense RNA; chromatin; supercoiling; transcription