title: Number and function impairment of resident C-Kit+ cardiac stem cells in mice with renal dysfunction caused by 5/6 nephrectomy.
abstract: BACKGROUND
  Cardiac stem cell (CSC) dysfunction exists in various kinds of cardiovascular diseases, and may be responsible for the insufficient regeneration of cardiac myocytes and coronary vessels. However, whether chronic renal failure (CRF) affected CSC is unknown.
  
  
  METHODS
  CRF was induced in adult male mice by 5/6 nephrectomy. The mice were killed at 12 weeks after operation. C-kit+ CSC numbers was evaluated by flow cytometer. Apoptosis and DNA damage of C-kit+ CSC in the control and CRF mice was analyzed by immunohistochemistry. In the in vitro study, normal medium, and medium with uremic rat serum were used for the CSC culture.
  
  
  RESULTS
  CSC counts attenuated significantly in the chronic renal failure model, whereas apoptosis cells and 8-OHdG-positive cells significantly increased. CSC derived form 5/6 nephrectomy mice showed an impaired anti-oxidant potential. In the cultured cells, CSCs subjected to uremic rat serum showed a higher frequency of TUNEL stain-positive and 8-OHdG-positive cells. The uremia rat serum reduced the expression of hepatocyte growth factor (HGF) and vascular endothelial growth factor (VEGF) in CSC.
  
  
  CONCLUSIONS
  The current study elucidated that CSC number and function disorders existed in mice with chronic renal insufficiency. Apoptosis, oxidative stress and reduced angiogenic factors secretion caused by uremic toxins in serum are contributors to CSC dysfunction.
authors.affiliation: Kidney Disease Center, The First Affiliated Hospital, Medical College of Zhejiang University, Hangzhou, Zhejiang, PR China.
authors.forename: Qiu
authors.initials: Q
authors.lastname: Jin
authors.forename: Ling
authors.initials: L
authors.lastname: Sun
authors.forename: Shiqi
authors.initials: S
authors.lastname: Li
authors.forename: Xian
authors.initials: X
authors.lastname: Yang
authors.forename: Yinyin
authors.initials: Y
authors.lastname: Lu
authors.forename: Yiwen
authors.initials: Y
authors.lastname: Li
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D017228:Hepatocyte Growth Factor / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D032383:Myocytes, Cardiac / Q000502:physiology*; D009392:Nephrectomy; D019009:Proto-Oncogene Proteins c-kit / Q000502:physiology*; D051436:Renal Insufficiency, Chronic / Q000209:etiology* / Q000473:pathology*; D013234:Stem Cells / Q000502:physiology*; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism
chemical_list: D042461:Vascular Endothelial Growth Factor A; D017228:Hepatocyte Growth Factor; D019009:Proto-Oncogene Proteins c-kit