title: Antioxidative and anti-inflammatory activities of paeoniflorin and oxypaeoniflora on AGEs-induced mesangial cell damage.
abstract: Paeonia suffruticosa, an important traditional herbal medicine, has been reported to prevent the pathogenesis of diabetic nephropathy through modulating advanced glycation end products-induced inflammatory and oxidative stress responses. However, little was known about the protective effect of the two major compounds in P. suffruticosa, paeoniflorin and oxypaeoniflora, on advanced glycation end products-induced mesangial cell damage. In the present study, we investigated the protective activities of paeoniflorin and oxypaeoniflora on advanced glycation end product-induced oxidative stress and inflammation in mesangial cells HBZY-1. The IC50 values of paeoniflorin and oxypaeoniflora for inhibiting 2,2'-azinobis-(3-thylbenzothiazoline-6-sulfonic acid) formation were 4.197 × 10-4 M and 1.002 × 10-4 M, respectively. The pretreatment with paeoniflorin and oxypaeoniflora (10-8-10-4 M) significantly increased advanced glycation end product-induced glutathione peroxidase and catalase activities. In the coculture system of HBZY-1 and macrophages, paeoniflorin and oxypaeoniflora could inhibit remarkably the migration of macrophages. Furthermore, paeniflorin and oxypaeniflora attenuated markedly advanced glycation end products-induced inflammation cytokines interleukin-6 and monocyte chemoattractant protein-1 levels in ELISA and western blot analysis in a dose-dependent manner. Taken together, our data provided the reliable evidence that paeniflorin and oxypaeniflora were able to attenuate advanced glycation end products-induced oxidative damage and inflammation in mesangial cells. Paeniflorin and oxypaeniflora might therefore have a beneficial effect in the treatment of diabetic nephropathy.
authors.affiliation: Key Laboratory of New Drug Delivery Systems of Chinese Materia Medica, Jiangsu Provincial Academy of Chinese Medicine, Jiangsu, Nanjing, P. R. China.
authors.forename: Ming-hua
authors.initials: MH
authors.lastname: Zhang
authors.forename: Liang
authors.initials: L
authors.lastname: Feng
authors.forename: Mao-mao
authors.initials: MM
authors.lastname: Zhu
authors.forename: Jun-fei
authors.initials: JF
authors.lastname: Gu
authors.forename: Chan
authors.initials: C
authors.lastname: Wu
authors.forename: Xiao-bin
authors.initials: XB
authors.lastname: Jia
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use; D000975:Antioxidants / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology* / Q000627:therapeutic use; D001565:Benzoates / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use; D001952:Bridged-Ring Compounds / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use; D002374:Catalase / Q000378:metabolism; D018932:Chemokine CCL2 / Q000378:metabolism; D003928:Diabetic Nephropathies / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use; D005960:Glucosides / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use; D005979:Glutathione Peroxidase / Q000378:metabolism; D017127:Glycation End Products, Advanced / Q000378:metabolism*; D007249:Inflammation / Q000188:drug therapy / Q000378:metabolism; D020128:Inhibitory Concentration 50; D015850:Interleukin-6 / Q000378:metabolism; D008264:Macrophages / Q000187:drug effects; D050527:Mesangial Cells / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D039821:Monoterpenes; D018384:Oxidative Stress / Q000187:drug effects; D029596:Paeonia / Q000737:chemistry*; D008517:Phytotherapy; D051381:Rats; D006027:Glycosides
chemical_list: D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D001565:Benzoates; D001952:Bridged-Ring Compounds; D002374:Catalase; D018932:Chemokine CCL2; D004365:Drugs, Chinese Herbal; D005960:Glucosides; D005979:Glutathione Peroxidase; D017127:Glycation End Products, Advanced; D015850:Interleukin-6; D039821:Monoterpenes; C015423:peoniflorin; D006027:Glycosides; C047593:oxypaeoniflora