title: A patterned recombinant human IgM guides neurite outgrowth of CNS neurons.
abstract: Matrix molecules convey biochemical and physical guiding signals to neurons in the central nervous system (CNS) and shape the trajectory of neuronal fibers that constitute neural networks. We have developed recombinant human IgMs that bind to epitopes on neural cells, with the aim of treating neurological diseases. Here we test the hypothesis that recombinant human IgMs (rHIgM) can guide neurite outgrowth of CNS neurons. Microcontact printing was employed to pattern rHIgM12 and rHIgM22, antibodies that were bioengineered to have variable regions capable of binding to neurons or oligodendrocytes, respectively. rHIgM12 promoted neuronal attachment and guided outgrowth of neurites from hippocampal neurons. Processes from spinal neurons followed grid patterns of rHIgM12 and formed a physical network. Comparison between rHIgM12 and rHIgM22 suggested the biochemistry that facilitates anchoring the neuronal surfaces is a prerequisite for the function of IgM, and spatial properties cooperate in guiding the assembly of neuronal networks.
authors.affiliation: Department of Neurology, Mayo Clinic College of Medicine, Rochester, MN 55905, USA.
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Xu
authors.forename: Nathan J
authors.initials: NJ
authors.lastname: Wittenberg
authors.forename: Luke R
authors.initials: LR
authors.lastname: Jordan
authors.forename: Shailabh
authors.initials: S
authors.lastname: Kumar
authors.forename: Jens O
authors.initials: JO
authors.lastname: Watzlawik
authors.forename: Arthur E
authors.initials: AE
authors.lastname: Warrington
authors.forename: Sang-Hyun
authors.initials: SH
authors.lastname: Oh
authors.forename: Moses
authors.initials: M
authors.lastname: Rodriguez
mesh_terms: D000818:Animals; D002490:Central Nervous System / Q000502:physiology; D005109:Extracellular Matrix; D006801:Humans; D007075:Immunoglobulin M / Q000494:pharmacology*; D051379:Mice; D016501:Neurites / Q000187:drug effects* / Q000502:physiology*; D009474:Neurons / Q000166:cytology / Q000187:drug effects* / Q000502:physiology*; D017966:Pyramidal Cells / Q000187:drug effects / Q000502:physiology; D011994:Recombinant Proteins / Q000494:pharmacology*
chemical_list: D007075:Immunoglobulin M; D011994:Recombinant Proteins