title: VE-cadherin status as an indicator of microvascular permeability.
abstract: VE-cadherin phosphorylation and binding partner status are important indicators of endothelial permeability. Here we describe several techniques aimed at discerning total tyrosine phosphorylation levels of VE-cadherin, VE-cadherin phosphorylation on specific tyrosine residues, and the ability of VE-cadherin to bind its binding partner beta-catenin. Taken together, these approaches to studying VE-cadherin status on microvascular endothelial cells are excellent complements to traditional permeability assays.
authors.affiliation: Department of Cell and Developmental Biology, Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Monaghan-Benson
authors.forename: Keith
authors.initials: K
authors.lastname: Burridge
mesh_terms: D015703:Antigens, CD / Q000235:genetics / Q000378:metabolism*; D015820:Cadherins / Q000235:genetics / Q000378:metabolism*; D002199:Capillary Permeability; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism; D006801:Humans; D008967:Molecular Biology / Q000379:methods*; D010766:Phosphorylation; D011485:Protein Binding; D014443:Tyrosine / Q000378:metabolism; D051176:beta Catenin / Q000378:metabolism; D000099152:Cadherin 5
chemical_list: D015703:Antigens, CD; D015820:Cadherins; D014443:Tyrosine; D051176:beta Catenin; D000099152:Cadherin 5