title: Perspectives for using genetically encoded fluorescent biosensors in plants.
abstract: Genetically encoded fluorescent biosensors have long proven to be excellent tools for quantitative live imaging, but sensor applications in plants have been lacking behind those in mammalian systems with respect to the variety of sensors and tissue types used. How can this be improved, and what can be expected for the use of genetically encoded fluorescent biosensors in plants in the future? In this review, we present a table of successful physiological experiments in plant tissue using fluorescent biosensors, and draw some conclusions about the specific challenges plant cell biologists are faced with and some of the ways they have been overcome so far.
authors.affiliation: Transport Biology Section, Department of Plant and Environmental Sciences, University of Copenhagen Copenhagen, Denmark.
authors.forename: Sisse K
authors.initials: SK
authors.lastname: Gjetting
authors.forename: Alexander
authors.initials: A
authors.lastname: Schulz
authors.forename: Anja T
authors.initials: AT
authors.lastname: Fuglsang
keywords: calcium sensor; fluorescence microscopy; genetically encoded biosensors; pH sensors; phosphatidylinositol sensor; plant bioimaging