title: Highly ordered protein nanorings designed by accurate control of glutathione S-transferase self-assembly.
abstract: Protein self-assembly into exquisite, complex, yet highly ordered architectures represents the supreme wisdom of nature. However, precise manipulation of protein self-assembly behavior in vitro is a great challenge. Here we report that by taking advantage of the cooperation of metal-ion-chelating interactions and nonspecific protein-protein interactions, we achieved accurate control of the orientation of proteins and their self-assembly into protein nanorings. As a building block, we utilized the C2-symmetric protein sjGST-2His, a variant of glutathione S-transferase from Schistosoma japonicum having two properly oriented His metal-chelating sites on the surface. Through synergic metal-coordination and non-covalent interactions, sjGST-2His self-assembled in a fixed bending manner to form highly ordered protein nanorings. The diameters of the nanorings can be regulated by tuning the strength of the non-covalent interaction network between sjGST-2His interfaces through variation of the ionic strength of the solution. This work provides a de novo design strategy that can be applied in the construction of novel protein superstructures.
authors.affiliation: State Key Laboratory of Supramolecular Structure and Materials, College of Chemistry, Jilin University, Changchun 130012, China.
authors.forename: Yushi
authors.initials: Y
authors.lastname: Bai
authors.forename: Quan
authors.initials: Q
authors.lastname: Luo
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: Lu
authors.initials: L
authors.lastname: Miao
authors.forename: Jiayun
authors.initials: J
authors.lastname: Xu
authors.forename: Hongbin
authors.initials: H
authors.lastname: Li
authors.forename: Junqiu
authors.initials: J
authors.lastname: Liu
mesh_terms: D000818:Animals; D002614:Chelating Agents / Q000737:chemistry; D015195:Drug Design; D005982:Glutathione Transferase / Q000737:chemistry*; D049329:Nanostructures / Q000737:chemistry*; D055503:Protein Multimerization; D020836:Protein Structure, Quaternary; D012549:Schistosoma japonicum / Q000201:enzymology
chemical_list: D002614:Chelating Agents; D005982:Glutathione Transferase