title: Determination and identification of hydrophilic and hydrophobic arsenic species in methanol extract of fresh cod liver by RP-HPLC with simultaneous ICP-MS and ESI-Q-TOF-MS detection.
abstract: The present study was focused on the determination and identification of arsenic species in methanolic extracts of cod liver. Arsenic species were fractionated and the fractions analysed by RP-HPLC-ICP-MS coupled with ESI-Q-TOF-MS. The total concentration of arsenic in the fresh cod liver was analysed by ICP-MS to be 1.53±0.02 mg As kg(-1)w.w. and the extraction recovery was ca. 100% and the column recovery >93%. Besides polar inorganic and methylated arsenic species (>70%) more hydrophobic arsenic-containing fatty acids and hydrocarbons occurred. Based on the mass spectrometric data proposals for molecular structures were elaborated for 20 of the organic As species included 10 arsenic-containing fatty acids (AsFA) and an arsenic-containing hydrocarbon (AsHC) mentioned for the first time in fresh cod liver. Arsenobetaine was found as main water-soluble arsenic compound in cod liver followed by higher molecular mass arsenic-containing fatty acids and hydrocarbons.
authors.affiliation: UFZ - Helmholtz Centre for Environmental Research, Department of Analytical Chemistry, Permoserstrasse 15, 04318 Leipzig, Germany.
authors.forename: Uriel
authors.initials: U
authors.lastname: Arroyo-Abad
authors.forename: Susanne
authors.initials: S
authors.lastname: Lischka
authors.forename: Christian
authors.initials: C
authors.lastname: Piechotta
authors.forename: Jürgen
authors.initials: J
authors.lastname: Mattusch
authors.forename: Thorsten
authors.initials: T
authors.lastname: Reemtsma
mesh_terms: D000818:Animals; D001151:Arsenic / Q000737:chemistry* / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005506:Food Contamination / Q000032:analysis*; D048389:Gadus morhua; D057927:Hydrophobic and Hydrophilic Interactions; D008099:Liver / Q000737:chemistry*; D017747:Seafood / Q000032:analysis*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D001151:Arsenic
keywords: Arsenic species; Arsenolipids; Cod liver; HPLC-ICP-MS/ESI-Q-TOF-MS; Marine fish