title: Passiflora manicata (Juss.) aqueous leaf extract protects against reactive oxygen species and protein glycation in vitro and ex vivo models.
abstract: The leaf extracts of many species of genus Passiflora have been extensively investigated for their biological activities on several rat tissues, but mainly in the central nervous system and liver. They posses anxiolytic-like, sedative effects and antioxidant properties. Evidences suggest a key role of C-glycosylflavonoids in the biological activities of Passiflora extracts. Some species (such as P. manicata) of the genus are still poorly investigated for their chemical and biological activity. In this work, we aim to investigate both antioxidant and antiglycation properties of aqueous extract of P. manicata leaves (PMLE) in vitro and ex vivo models. Crude extract showed the C-glycosylflavonoid isovitexin as the major compound. Isoorientin and vitexin were also identified. In TRAP/TAR assay, PMLE showed a significant antioxidant activity. PMLE at concentrations of 10 and 100 μg mL⁻¹ significantly decreasing LDH leakage in rat liver slices. Antioxidant effect also was observed by decreased in oxidative damage markers in slices hence hydrogen peroxide was added as oxidative stress inductor. PMLE inhibited protein glycation at all concentrations tested. In summary, P. manicata aqueous leaf extract possess protective properties against reactive oxygen species and also protein glycation, and could be considered a new source of natural antioxidants.
authors.affiliation: Centro de Estudos de Estresse Oxidativo, Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil. Electronic address: maurilio.bio@gmail.com.
authors.forename: Maurilio
authors.initials: M
authors.lastname: da Silva Morrone
authors.forename: Adriano Martimbianco
authors.initials: AM
authors.lastname: de Assis
authors.forename: Ricardo Fagundes
authors.initials: RF
authors.lastname: da Rocha
authors.forename: Juciano
authors.initials: J
authors.lastname: Gasparotto
authors.forename: Andressa Córneo
authors.initials: AC
authors.lastname: Gazola
authors.forename: Geison Modesti
authors.initials: GM
authors.lastname: Costa
authors.forename: Silvana Maria
authors.initials: SM
authors.lastname: Zucolotto
authors.forename: Leonardo H
authors.initials: LH
authors.lastname: Castellanos
authors.forename: Freddy A
authors.initials: FA
authors.lastname: Ramos
authors.forename: Eloir Paulo
authors.initials: EP
authors.lastname: Schenkel
authors.forename: Flávio Henrique
authors.initials: FH
authors.lastname: Reginatto
authors.forename: Daniel Pens
authors.initials: DP
authors.lastname: Gelain
authors.forename: José C F
authors.initials: JC
authors.lastname: Moreira
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D047310:Apigenin / Q000494:pharmacology; D005979:Glutathione Peroxidase / Q000378:metabolism; D006861:Hydrogen Peroxide; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D047311:Luteolin / Q000494:pharmacology; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects; D029598:Passiflora / Q000737:chemistry*; D010936:Plant Extracts / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry*; D050050:Protein Carbonylation; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis
chemical_list: D000975:Antioxidants; D010936:Plant Extracts; D017382:Reactive Oxygen Species; D017392:Thiobarbituric Acid Reactive Substances; D047310:Apigenin; C032731:vitexin; C057912:homoorientin; D006861:Hydrogen Peroxide; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; C049772:isovitexin; D047311:Luteolin
keywords: 2,2-azobis[2-amidinopropane]; 5,5-dithionitrobis 2-nitrobenzoic acid; AAPH; ANOVA; AUC; Antioxidant; BSA; C-glycosylflavonoids; CAT; CDNB; CNS; DNPH; DTNB; GPx; H(2)O(2); HPLC-UV DAD; LDH; Oxidative stress; PMLE; Passiflora manicata; Passiflora manicata leaf extract; Protein glycation; SOD; TAR; TBARS; TRAP; analysis of variance; area under curve; bovine serum albumin; catalase; central nervous system; chloro-dinitro benzene; dinitrophenylhidrazine; glutathione peroxidase; high performance liquid chromatography equipped with Diode Array Detection; hydrogen peroxide; lactate dehydrogenase; superoxide dismutase; thiobarbituric reactive species; total antioxidant reactivity; total reactive antioxidant potential