title: Isolation of a high malic and low acetic acid-producing sake yeast Saccharomyces cerevisiae strain screened from respiratory inhibitor 2,4-dinitrophenol (DNP)-resistant strains.
abstract: We isolated 2,4-dinitrophenol (DNP)-resistant sake yeast strains by UV mutagenesis. Among the DNP-resistant mutants, we focused on strains exhibiting high malic acid and low acetic acid production. The improved organic acid composition is unlikely to be under the control of enzyme activities related to malic and acetic acid synthesis pathways. Instead, low mitochondrial activity was observed in DNP-resistant mutants, indicating that the excess pyruvic acid generated during glycolysis is not metabolized in the mitochondria but converted to malic acid in the cytosol. In addition, the NADH/NAD(+) ratio of the DNP-resistant strains was higher than that of the parental strain K901. These results suggest that the increased NADH/NAD(+) ratio together with the low mitochondrial activity alter the organic acid composition because malic acid synthesis requires NADH, while acetic acid uses NAD(+).
authors.affiliation: Department of Fermentation Science and Technology, Faculty of Applied Bio-science, Tokyo University of Agriculture, Sakuragaoka 1-1-1, Setagaya-ku, Tokyo 156-8502, Japan.
authors.forename: Shingo
authors.initials: S
authors.lastname: Kosugi
authors.affiliation: Department of Fermentation Science and Technology, Faculty of Applied Bio-science, Tokyo University of Agriculture, Sakuragaoka 1-1-1, Setagaya-ku, Tokyo 156-8502, Japan.
authors.forename: Keiji
authors.initials: K
authors.lastname: Kiyoshi
authors.affiliation: Biotechnology and Food Research Institute, Fukuoka Industrial Technology Center, 1465-5 Kurume, Fukuoka 839-0861, Japan.
authors.forename: Takahiro
authors.initials: T
authors.lastname: Oba
authors.affiliation: Biotechnology and Food Research Institute, Fukuoka Industrial Technology Center, 1465-5 Kurume, Fukuoka 839-0861, Japan.
authors.forename: Kenichi
authors.initials: K
authors.lastname: Kusumoto
authors.affiliation: Department of Fermentation Science and Technology, Faculty of Applied Bio-science, Tokyo University of Agriculture, Sakuragaoka 1-1-1, Setagaya-ku, Tokyo 156-8502, Japan.
authors.forename: Toshimori
authors.initials: T
authors.lastname: Kadokura
authors.affiliation: Department of Fermentation Science and Technology, Faculty of Applied Bio-science, Tokyo University of Agriculture, Sakuragaoka 1-1-1, Setagaya-ku, Tokyo 156-8502, Japan.
authors.forename: Atsumi
authors.initials: A
authors.lastname: Nakazato
authors.affiliation: Department of Fermentation Science and Technology, Faculty of Applied Bio-science, Tokyo University of Agriculture, Sakuragaoka 1-1-1, Setagaya-ku, Tokyo 156-8502, Japan. Electronic address: s3nakaya@nodai.ac.jp.
authors.forename: Shunichi
authors.initials: S
authors.lastname: Nakayama
mesh_terms: D019297:2,4-Dinitrophenol / Q000494:pharmacology*; D019342:Acetic Acid / Q000378:metabolism*; D003600:Cytosol / Q000378:metabolism; D025141:Drug Resistance, Fungal; D000431:Ethanol / Q000378:metabolism; D008293:Malates / Q000378:metabolism*; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism*; D016296:Mutagenesis; D009243:NAD / Q000378:metabolism; D019289:Pyruvic Acid / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D008293:Malates; D009243:NAD; D000431:Ethanol; C030298:malic acid; D019289:Pyruvic Acid; D019297:2,4-Dinitrophenol; D019342:Acetic Acid
keywords: Mitochondria; Organic acids; Redox balance; Respiration inhibitor; Sake yeast