title: Resveratrol prevents high-fructose corn syrup-induced vascular insulin resistance and dysfunction in rats.
abstract: Dietary intake of fructose and sucrose can cause development of metabolic and cardiovascular disorders. The consequences of high-fructose corn syrup (HFCS), a commonly consumed form of fructose and glucose, have poorly been examined. Therefore, in this study, we investigated whether HFCS intake (10% and 20% beverages for 12 weeks) impacts vascular reactivity to insulin and endothelin-1 in conjunction with insulin receptor substrate-1(IRS-1), endothelial nitric oxide synthase (eNOS) and inducible NOS (iNOS) mRNA/proteins levels in aorta of rats. At challenge, we tested the effectiveness of resveratrol (28-30 mg/kg body weight/day) on outcomes of HFCS feeding. HFCS (20%) diet feeding increased plasma triglyceride, VLDL, cholesterol, insulin and glucose levels, but not body weights of rats. Impaired nitric oxide-mediated relaxation to insulin (10⁻⁹ to 3×10⁻⁶ M), and enhanced contraction to endothelin-1 (10⁻¹¹ to 10⁻⁸ M) were associated with decreased expression of IRS-1 and eNOS mRNA and protein, but increased expression of iNOS, in aortas of rats fed with HFCS. Resveratrol supplementation restored many features of HFCS-induced disturbances, probably by regulating eNOS and iNOS production. In conclusion, dietary HFCS causes vascular insulin resistance and endothelial dysfunction through attenuating IRS-1 and eNOS expressions as well as increasing iNOS in rats. Resveratrol has capability to recover HFCS-induced disturbances.
authors.affiliation: Department of Pharmacology, Faculty of Pharmacy, Gazi University, Ankara, Turkey.
authors.forename: C
authors.initials: C
authors.lastname: Babacanoglu
authors.forename: N
authors.initials: N
authors.lastname: Yildirim
authors.forename: G
authors.initials: G
authors.lastname: Sadi
authors.forename: M B
authors.initials: MB
authors.lastname: Pektas
authors.forename: F
authors.initials: F
authors.lastname: Akar
mesh_terms: D000818:Animals; D001835:Body Weight / Q000187:drug effects; D015243:Cholesterol, VLDL / Q000097:blood; D019332:Endothelin-1 / Q000097:blood; D005632:Fructose / Q000008:administration & dosage / Q000009:adverse effects*; D015870:Gene Expression; D007328:Insulin / Q000097:blood; D055504:Insulin Receptor Substrate Proteins / Q000235:genetics / Q000378:metabolism; D007333:Insulin Resistance; D008297:Male; D052247:Nitric Oxide Synthase Type II / Q000235:genetics / Q000378:metabolism; D052250:Nitric Oxide Synthase Type III / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D000077185:Resveratrol; D013267:Stilbenes / Q000494:pharmacology*; D013549:Sweetening Agents / Q000008:administration & dosage / Q000009:adverse effects*; D014280:Triglycerides / Q000097:blood
chemical_list: D015243:Cholesterol, VLDL; D019332:Endothelin-1; D007328:Insulin; D055504:Insulin Receptor Substrate Proteins; C526296:Irs1 protein, rat; D012333:RNA, Messenger; D013267:Stilbenes; D013549:Sweetening Agents; D014280:Triglycerides; D005632:Fructose; D052247:Nitric Oxide Synthase Type II; D052250:Nitric Oxide Synthase Type III; C496318:Nos2 protein, rat; C496316:Nos3 protein, rat; D000077185:Resveratrol
keywords: Endothelin; High-fructose corn syrup; IRS-1; Resveratrol; Vascular insulin resistance; eNOS