title: Human dermal stem/progenitor cell-derived conditioned medium ameliorates ultraviolet a-induced damage of normal human dermal fibroblasts.
abstract: Adult skin stem cells are considered an attractive cell resource for therapeutic potential in aged skin. We previously reported that multipotent human dermal stem/progenitor cells (hDSPCs) can be enriched from (normal human dermal fibroblasts (NHDFs) using collagen type IV. However, the beneficial effects of hDSPCs on aged skin remain to be elucidated. In the present study, we analyzed the growth factors secreted from hDSPCs in conditioned medium (CM) derived from hDSPCs (hDSPC-CM) and found that hDSPCs secreted higher levels of bFGF, IGFBP-1, IGFBP-2, HGF, VEGF and IGF-1 compared with non-hDSPCs. We then investigated whether hDSPC-CM has an effect on ultraviolet A (UVA)-irradiated NHDFs. Real-time RT-PCR analysis revealed that the treatment of UVA-irradiated NHDFs with hDSPC-CM significantly antagonized the UVA-induced up-regulation of the MMP1 and the UVA-induced down-regulation of the collagen types I, IV and V and TIMP1 mRNA expressions. Furthermore, a scratch wound healing assay showed that hDSPC-CM enhanced the migratory properties of UVA-irradiated NHDFs. hDSPC-CM also significantly reduced the number of the early and late apoptotic cell population in UVA-irradiated NHDFs. Taken together, these data suggest that hDSPC-CM can exert some beneficial effects on aged skin and may be used as a therapeutic agent to improve skin regeneration and wound healing.
authors.affiliation: Bioscience Research Institute, Amorepacific Corporation R&D Center, Yongin-city, Gyeonggi-do, Republic of Korea.
authors.forename: Joong Hyun
authors.initials: JH
authors.lastname: Shim
authors.forename: Ju-Yearl
authors.initials: JY
authors.lastname: Park
authors.forename: Mi-Gi
authors.initials: MG
authors.lastname: Lee
authors.forename: Hak Hee
authors.initials: HH
authors.lastname: Kang
authors.forename: Tae Ryong
authors.initials: TR
authors.lastname: Lee
authors.forename: Dong Wook
authors.initials: DW
authors.lastname: Shin
mesh_terms: D017209:Apoptosis / Q000235:genetics; D002478:Cells, Cultured; D003094:Collagen / Q000235:genetics / Q000378:metabolism; D017077:Culture Media, Conditioned; D016207:Cytokines / Q000235:genetics / Q000378:metabolism; D015536:Down-Regulation; D005347:Fibroblasts / Q000378:metabolism; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism; D020781:Matrix Metalloproteinase 1 / Q000235:genetics / Q000378:metabolism; D012867:Skin / Q000166:cytology* / Q000378:metabolism* / Q000528:radiation effects; D015595:Skin Aging / Q000187:drug effects; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism* / Q000528:radiation effects; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000235:genetics / Q000378:metabolism; D014466:Ultraviolet Rays; D015854:Up-Regulation; D014945:Wound Healing / Q000502:physiology
chemical_list: D017077:Culture Media, Conditioned; D016207:Cytokines; D036341:Intercellular Signaling Peptides and Proteins; C560822:TIMP1 protein, human; D019715:Tissue Inhibitor of Metalloproteinase-1; D003094:Collagen; D020781:Matrix Metalloproteinase 1