title: Immunogenicity of two FMDV nonameric peptides encapsulated in liposomes in mice and the protective efficacy in guinea pigs.
abstract: It has been predicted that nonameric peptides I (VP1(26-34), RRQHTDVSF), II (VP1(157-165), RTLPTSFNY) and III (VP1(45-53), KEQVNVLDL) from the VP1 capsid protein of the foot-and-mouth disease virus (FMDV) are T cell epitopes. To investigate whether these peptides have immunological activity, BALB/c mice were immunized with peptide I, II or III conjugated with immunostimulating complexes (ISCOMs). A cytotoxic T lymphocyte assay was used to evaluate the cytotoxic activity induced by peptides along with by measuring peptide-specific T-cell proliferation and CD8(+) T lymphocyte numbers in whole blood and interferon (IFN)-γ production in peripheral blood mononuclear cells induced by peptides. To further identify the protective efficacy of peptides, an FMDV challenge assay was done in guinea pigs. Peptides I and II stimulated significant increases in T-cell proliferation, CD8(+) T lymphocytes, and IFN-γ secretion and cytotoxic activity compared to controls. The FMDV challenge assay indicated peptides I and II can protect over 60% of animals from virus attack. The results demonstrate that peptides I and II encapsulated in liposomes should be CTL epitopes of FMDV and can protect animals from virus attack to some extent.
authors.affiliation: Department of Biochemistry and Molecular Biology, College of Life Science and Technology, Dalian University, Dalian, Liaoning, China. gfsh0626@126.com
authors.forename: Feng-Shan
authors.initials: FS
authors.lastname: Gao
authors.forename: Lei
authors.initials: L
authors.lastname: Feng
authors.forename: Qiang
authors.initials: Q
authors.lastname: Zhang
authors.forename: Ruo-qian
authors.initials: RQ
authors.lastname: Yan
authors.forename: Yun-Gang
authors.initials: YG
authors.lastname: Li
authors.forename: Xin-sheng
authors.initials: XS
authors.lastname: Li
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D036022:Capsid Proteins / Q000737:chemistry / Q000276:immunology*; D018984:Epitopes, T-Lymphocyte / Q000737:chemistry / Q000276:immunology; D005260:Female; D005536:Foot-and-Mouth Disease / Q000276:immunology* / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control; D029544:Foot-and-Mouth Disease Virus / Q000276:immunology*; D006168:Guinea Pigs; D007371:Interferon-gamma / Q000276:immunology / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000276:immunology / Q000378:metabolism; D008081:Liposomes; D008297:Male; D051379:Mice; D010455:Peptides / Q000737:chemistry / Q000276:immunology*; D016176:T-Lymphocyte Subsets / Q000276:immunology; D014765:Viral Vaccines / Q000276:immunology*
chemical_list: D036022:Capsid Proteins; D018984:Epitopes, T-Lymphocyte; D008081:Liposomes; D010455:Peptides; C442501:VP1 protein, Foot-and-mouth disease virus; D014765:Viral Vaccines; D007371:Interferon-gamma