title: Flat-panel volumetric computed tomography in cerebral perfusion: evaluation of three rat stroke models.
abstract: Flat-panel volumetric computed tomography (fpVCT) is a non-invasive approach to three-dimensional small animal imaging. The capability of volumetric scanning and a high resolution in time and space enables whole organ perfusion studies. We aimed to assess feasibility and validity of fpVCT in cerebral perfusion measurement with impaired hemodynamics by evaluation of three well-established rat stroke models for temporary and permanent middle cerebral artery occlusion (MCAO). Male Wistar rats were randomly assigned to temporary (group I: suture model) and permanent (group II: suture model; III: macrosphere model) MCAO and to a control group. Perfusion scans with respect to cerebral blood flow (CBF) and volume (CBV) were performed 24h post intervention by fpVCT, using a Gantry rotation time of 1s and a total scanning time of 30s. Postmortem analysis included infarct-size calculation by 2,3,5-triphenyltetrazolium chloride (TTC) staining. Infarct volumes did not differ significantly throughout intervention groups. After permanent MCAO, CBF significantly decreased in subcortical regions to 78.2% (group II, p=0.005) and 79.9% (group III, p=0.012) and in total hemisphere to 77.4% (group II, p=0.010) and 82.0% (group III, p=0.049). CBF was less impaired with temporary vessel occlusion. CBV measurement revealed no significant differences. Results demonstrate feasibility of cerebral perfusion quantification in rats with the fpVCT, which can be a useful tool for non-invasive dynamic imaging of cerebral perfusion in rodent stroke models. In addition to methodological advantages, CBF data confirm the macrosphere model as a useful alternative to the suture model for permanent experimental MCAO.
authors.affiliation: Department of Neurology, Justus-Liebig-University Giessen, Klinikstrasse 33, 35392 Giessen, Germany. martin.juenemann@neuro.med.uni-giessen.de
authors.forename: Martin
authors.initials: M
authors.lastname: Juenemann
authors.forename: Sinja
authors.initials: S
authors.lastname: Goegel
authors.forename: Martin
authors.initials: M
authors.lastname: Obert
authors.forename: Nadine
authors.initials: N
authors.lastname: Schleicher
authors.forename: Nouha
authors.initials: N
authors.lastname: Ritschel
authors.forename: Simone
authors.initials: S
authors.lastname: Doenges
authors.forename: Inka
authors.initials: I
authors.lastname: Eitenmueller
authors.forename: Niko
authors.initials: N
authors.lastname: Schwarz
authors.forename: Sabrina
authors.initials: S
authors.lastname: Kastaun
authors.forename: Mesut
authors.initials: M
authors.lastname: Yeniguen
authors.forename: Marlene
authors.initials: M
authors.lastname: Tschernatsch
authors.forename: Tibo
authors.initials: T
authors.lastname: Gerriets
mesh_terms: D000465:Algorithms; D000818:Animals; D002560:Cerebrovascular Circulation / Q000502:physiology*; D054893:Cone-Beam Computed Tomography / Q000379:methods*; D007839:Functional Laterality / Q000502:physiology; D006439:Hemodynamics / Q000502:physiology; D007091:Image Processing, Computer-Assisted; D020244:Infarction, Middle Cerebral Artery / Q000503:physiopathology; D008297:Male; D004856:Postural Balance / Q000502:physiology; D011597:Psychomotor Performance / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D020521:Stroke / Q000000981:diagnostic imaging* / Q000503:physiopathology*
keywords: Animal model; Cerebral perfusion; Flat-panel volume CT; Stroke