title: Glucose microfluidic biosensors based on reversible enzyme immobilization on photopatterned stimuli-responsive polymer.
abstract: In this paper, we demonstrate a new strategy for replaceable enzymatic microreactor based on a switchable wettability interface of poly(N-isopropylacrylamide) (PNIPAAm). PNIPAAm porous polymer monolith (PPM) with 3D macroporous framework is photopolymerized in glass microchip within 30 s. The PNIPAAm PPM not only shows its reversible swelling/shrinking property at the different temperature around the lower critical solution temperature (LCST), but also shows reversible hydrophilicity/hydrophobicity corresponding to its swelling/shrinking status. Based on these properties, a biocompatible and replaceable on-chip enzymatic microreactor has been successfully built by means of the reversible adsorption and release of glucose oxidase (GOx) on the robust and stable matrix. Coupled with a carbon fiber microelectrode as electrochemical detector, the microreactor has been successfully employed for detection of glucose with a linear range from 0.05 to 5 mM. This approach may provide a promising way for high efficient and renewable microreactors that will find wide application in clinical diagnosis, biochemical synthesis/analysis, and proteomic research.
authors.affiliation: State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, China.
authors.forename: Meng
authors.initials: M
authors.lastname: Xiong
authors.forename: Bin
authors.initials: B
authors.lastname: Gu
authors.forename: Jia-Dong
authors.initials: JD
authors.lastname: Zhang
authors.forename: Jing-Juan
authors.initials: JJ
authors.lastname: Xu
authors.forename: Hong-Yuan
authors.initials: HY
authors.lastname: Chen
authors.forename: Hui
authors.initials: H
authors.lastname: Zhong
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry*; D000327:Adsorption; D015374:Biosensing Techniques / Q000295:instrumentation*; D001786:Blood Glucose / Q000032:analysis*; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism*; D004867:Equipment Design; D005949:Glucose Oxidase / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D057230:Limit of Detection; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D016062:Porosity
chemical_list: D000180:Acrylic Resins; D001786:Blood Glucose; D004800:Enzymes, Immobilized; C052970:poly-N-isopropylacrylamide; D005949:Glucose Oxidase
keywords: Amperometry; Enzyme immobilization; Microfluidics; Photopatterning; Poly(N-isopropylacrylamide)