title: Mapping of PARK2 and PACRG overlapping regulatory region reveals LD structure and functional variants in association with leprosy in unrelated indian population groups.
abstract: Leprosy is a chronic infectious disease caused by Mycobacterium Leprae, where the host genetic background plays an important role toward the disease pathogenesis. Various studies have identified a number of human genes in association with leprosy or its clinical forms. However, non-replication of results has hinted at the heterogeneity among associations between different population groups, which could be due to differently evolved LD structures and differential frequencies of SNPs within the studied regions of the genome. A need for systematic and saturated mapping of the associated regions with the disease is warranted to unravel the observed heterogeneity in different populations. Mapping of the PARK2 and PACRG gene regulatory region with 96 SNPs, with a resolution of 1 SNP per 1 Kb for PARK2 gene regulatory region in a North Indian population, showed an involvement of 11 SNPs in determining the susceptibility towards leprosy. The association was replicated in a geographically distinct and unrelated population from Orissa in eastern India. In vitro reporter assays revealed that the two significantly associated SNPs, located 63.8 kb upstream of PARK2 gene and represented in a single BIN of 8 SNPs, influenced the gene expression. A comparison of BINs between Indian and Vietnamese populations revealed differences in the BIN structures, explaining the heterogeneity and also the reason for non-replication of the associated genomic region in different populations.
authors.affiliation: Shri Mata Vaishno Devi University, School of Biotechnology, Katra, Jammu & Kashmir, India.
authors.forename: Rupali
authors.initials: R
authors.lastname: Chopra
authors.forename: Shafat
authors.initials: S
authors.lastname: Ali
authors.forename: Amit K
authors.initials: AK
authors.lastname: Srivastava
authors.forename: Shweta
authors.initials: S
authors.lastname: Aggarwal
authors.forename: Bhupender
authors.initials: B
authors.lastname: Kumar
authors.forename: Siddharth
authors.initials: S
authors.lastname: Manvati
authors.forename: Ponnusamy
authors.initials: P
authors.lastname: Kalaiarasan
authors.forename: Mamta
authors.initials: M
authors.lastname: Jena
authors.forename: Vijay K
authors.initials: VK
authors.lastname: Garg
authors.forename: Sambit N
authors.initials: SN
authors.lastname: Bhattacharya
authors.forename: Rameshwar N K
authors.initials: RN
authors.lastname: Bamezai
mesh_terms: D044466:Asian People / Q000235:genetics; D002874:Chromosome Mapping; D005786:Gene Expression Regulation; D056726:Genetic Association Studies; D018740:Genetic Heterogeneity; D020022:Genetic Predisposition to Disease; D006239:Haplotypes; D006801:Humans; D007194:India; D007918:Leprosy / Q000235:genetics* / Q000382:microbiology / Q000473:pathology; D008840:Microfilament Proteins; D018832:Molecular Chaperones / Q000235:genetics*; D009166:Mycobacterium leprae / Q000472:pathogenicity; D020641:Polymorphism, Single Nucleotide; D012045:Regulatory Sequences, Nucleic Acid; D044767:Ubiquitin-Protein Ligases / Q000235:genetics*
chemical_list: D008840:Microfilament Proteins; D018832:Molecular Chaperones; C471711:PACRG protein, human; D044767:Ubiquitin-Protein Ligases; C111567:parkin protein