title: Robust optimization of psychotropic drug mixture separation in hydrophilic interaction liquid chromatography.
abstract: This paper presents multiobjective optimization of complex mixtures separation in hydrophilic interaction liquid chromatography (HILIC). The selected model mixture consisted of five psychotropic drugs: clozapine, thioridazine, sulpiride, pheniramine and lamotrigine. Three factors related to the mobile phase composition (acetonitrile content, pH of the water phase and concentration of ammonium acetate) were optimized in order to achieve the following goals: maximal separation quality, minimal total analysis duration and robustness of an optimum. The consideration of robustness in early phases of the method development provides reliable methods with low risk for failure in validation phase. The simultaneous optimization of all goals was achieved by multiple threshold approach combined with grid point search. The identified optimal separation conditions (acetonitrile content 83%, pH of the water phase 3.5 and ammonium acetate content in water phase 14 mM) were experimentally verified.
authors.affiliation: University of Belgrade - Faculty of Pharmacy, Department of Drug Analysis, Vojvode Stepe 450, Belgrade, Serbia.
authors.forename: Tijana
authors.initials: T
authors.lastname: Rakić
authors.forename: Marko
authors.initials: M
authors.lastname: Jovanović
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Dumić
authors.forename: Marina
authors.initials: M
authors.lastname: Pekić
authors.forename: Sanja
authors.initials: S
authors.lastname: Ribić
authors.forename: Biljana Jancić
authors.initials: BJ
authors.lastname: Stojanović
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D011619:Psychotropic Drugs / Q000302:isolation & purification*
chemical_list: D011619:Psychotropic Drugs