title: Arsenic-tolerant, arsenite-oxidising bacterial strains in the contaminated soils of West Bengal, India.
abstract: As biological agents represent an affordable alternative to costly metal decontamination technologies, we isolated arsenic (As) oxidising bacteria from the As-contaminated soils of West Bengal, India. These strains were closely related to various species of Bacillus and Geobacillus based on their 16S rRNA gene sequences. They were found to be hyper-resistant to both As(V) (167-400 mM) and As(III) (16-47 mM). Elevated rates of As(III) oxidation (278-1250 μM h(-1)) and arsenite oxidase activity (2.1-12.5 nM min(-1) mg(-1) protein) were observed in these isolates. Screening identified four strains as superior As-oxidisers. Among them, AMO-10 completely (100%) oxidised 30 mM of As(III) within 24 h. The presence of the aoxB gene was confirmed in the screened isolates. Phylogenetic tree construction based on the aoxB sequence revealed that two strains, AGO-S5 and AGH-02, clustered with Achromobacter and Variovorax, whereas the other two (AMO-10 and ADP-25) remained unclustered. The increased rate of As(III) oxidation by these native strains might be exploited for the remediation of As in contaminated environments. Notably, this study presents the first correlation regarding the presence of the aoxB gene and As(III) oxidation ability in Geobacillus stearothermophilus.
authors.affiliation: Arsenic Research Laboratory, Bidhan Chandra Krishi Viswavidyalaya, Kalyani 741235, West Bengal, India. Electronic address: aparajita_micro@yahoo.co.in.
authors.forename: Aparajita
authors.initials: A
authors.lastname: Majumder
authors.forename: K
authors.initials: K
authors.lastname: Bhattacharyya
authors.forename: S
authors.initials: S
authors.lastname: Bhattacharyya
authors.forename: S C
authors.initials: SC
authors.lastname: Kole
mesh_terms: D001151:Arsenic / Q000378:metabolism*; D018053:Arsenites / Q000378:metabolism*; D001407:Bacillus / Q000235:genetics / Q000378:metabolism; D001419:Bacteria / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D004787:Environmental Pollution; D007194:India; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012988:Soil Microbiology
chemical_list: D018053:Arsenites; D012336:RNA, Ribosomal, 16S; C015001:arsenite; D001151:Arsenic
keywords: Arsenic oxidising bacteria; Bacillus and Geobacillus; Soil; aoxB