title: Nuclear localization of aldolase A correlates with cell proliferation.
abstract: Muscle fructose 1,6-bisphosphate aldolase (ALDA) is a glycolytic enzyme which may localize both in nuclei and cytoplasm of cells, however its role in the nuclei is unclear. Here, we demonstrate the links between subcellular localization of ALDA and the cell cycle progression as well as the availability of energetic substrates. Results of our studies indicate that nuclear localization of ALDA correlates with the proliferative activity of the cells and with the expression of Ki-67, a marker of proliferation, both in the KLN-205 (mouse lung cancer cells) and human squamous cell lung cancer cells (hSCC). Chemically-induced block of cell cycle entry in S phase and the inhibition of transcription stimulate removal of ALDA from cells nuclei suggesting that nuclear ALDA is involved in cells proliferation. On the other hand, subcellular distribution of the enzyme also depends on the stress and pro-survival signals mediated by the Akt and the p38 pathways and, in non-proliferating cells, on the availability of glucose and lactate. The results presented here point to ALDA as a factor involved in the regulation of cells proliferation.
authors.affiliation: Department of Animal Molecular Physiology, Institute of Experimental Biology, Wroclaw University, Cybulskiego 30, 50-205 Wroclaw, Poland.
authors.forename: Piotr
authors.initials: P
authors.lastname: Mamczur
authors.affiliation: Department of Medical Biochemistry, Wroclaw Medical University, Chalubinskiego 10, 50-368,Wroclaw, Poland; Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Weigla 12, 53-114 Wrocław, Poland.
authors.forename: Andrzej
authors.initials: A
authors.lastname: Gamian
authors.affiliation: Department of Thoracic Surgery, Wroclaw Medical University, Grabiszyńska 105, 50-439 Wroclaw, Poland.
authors.forename: Jerzy
authors.initials: J
authors.lastname: Kolodziej
authors.affiliation: Department of Histology and Embryology, Wroclaw Medical University, Chalubinskiego 6a, 50-368 Wroclaw, Poland.
authors.forename: Piotr
authors.initials: P
authors.lastname: Dziegiel
authors.affiliation: Department of Animal Molecular Physiology, Institute of Experimental Biology, Wroclaw University, Cybulskiego 30, 50-205 Wroclaw, Poland. Electronic address: drakus@biol.uni.wroc.pl.
authors.forename: Dariusz
authors.initials: D
authors.lastname: Rakus
mesh_terms: D000818:Animals; D016590:Aphidicolin / Q000494:pharmacology; D002452:Cell Count; D045744:Cell Line, Tumor; D002467:Cell Nucleus / Q000187:drug effects / Q000201:enzymology*; D049109:Cell Proliferation / Q000187:drug effects; D003208:Concanavalin A / Q000494:pharmacology; D003600:Cytosol / Q000201:enzymology; D003609:Dactinomycin / Q000494:pharmacology; D015536:Down-Regulation / Q000187:drug effects; D004734:Energy Metabolism / Q000187:drug effects; D005634:Fructose-Bisphosphate Aldolase / Q000378:metabolism*; D006801:Humans; D019394:Ki-67 Antigen / Q000378:metabolism; D051379:Mice; D016376:Oligonucleotides, Antisense / Q000494:pharmacology; D010766:Phosphorylation / Q000187:drug effects; D047428:Protein Kinase Inhibitors / Q000494:pharmacology; D021381:Protein Transport / Q000187:drug effects; D051057:Proto-Oncogene Proteins c-akt / Q000037:antagonists & inhibitors / Q000378:metabolism; D011817:Rabbits; D016192:Resting Phase, Cell Cycle / Q000187:drug effects; D044967:Serum / Q000378:metabolism; D048051:p38 Mitogen-Activated Protein Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism
chemical_list: D019394:Ki-67 Antigen; D016376:Oligonucleotides, Antisense; D047428:Protein Kinase Inhibitors; D003208:Concanavalin A; D003609:Dactinomycin; D016590:Aphidicolin; D051057:Proto-Oncogene Proteins c-akt; D048051:p38 Mitogen-Activated Protein Kinases; D005634:Fructose-Bisphosphate Aldolase
keywords: ACD; ALDA; ALDC; APC; Aldolase; ConA; KLN-205; Ki-67; LNA-oligo; Squamous cell lung cancer; actinomycin D; aphidicolin; brain aldolase isozyme; concanavalin A; hSCC; human squamous cell lung cancer; locked nucleic acid antisense oligonucleotide; muscle aldolase isozyme