title: Expression profiles of Toll-like receptors 1, 2 and 5 in selected organs of commercial and indigenous chickens.
abstract: Toll-like receptors (TLRs) are members of the cellular receptors that constitute a major component of the evolutionary conserved pattern recognition system (PRR). TLRs are expressed in a wide variety of tissues and cell types. In this study we compared the expression profiles of the chicken TLR1, TLR2 and TLR5 genes in a range of organs (lung, ovary, liver, thymus, duodenum, spleen and large intestine) in commercial Hy-Line (HL) and indigenous Green-legged Partridgelike (GP) chickens. The level of mRNA was determined with RT-qPCR using the TaqMan probes for target and reference (ACTB) genes. We determined that the tissue profiles differed with respect to each TLR and they were ranked as follows: spleen, lungs, large intestine (TLR1), large intestine, lungs, thymus/ovary (TLR2) and lungs, thymus, liver (TLR5). A differential expression between HL and GP chickens was determined for TLR1 and TLR5 genes in large intestine and thymus of HL (P < 0.05) and GP (P < 0.05) chickens. We conclude that the commercial chickens expressed higher levels of TLR1 mRNA in large intestine and TLR5 mRNA in thymus than indigenous chickens.
authors.affiliation: Department of Animal Biotechnology and Histology, University of Technology and Life Sciences in Bydgoszcz, Mazowiecka 28, 85-225, Bydgoszcz, Poland, slawinska@utp.edu.pl.
authors.forename: Anna
authors.initials: A
authors.lastname: Sławińska
authors.forename: Mariasilvia
authors.initials: M
authors.lastname: D'Andrea
authors.forename: Fabio
authors.initials: F
authors.lastname: Pilla
authors.forename: Marek
authors.initials: M
authors.lastname: Bednarczyk
authors.forename: Maria
authors.initials: M
authors.lastname: Siwek
mesh_terms: D000818:Animals; D002645:Chickens / Q000235:genetics*; D005260:Female; D020869:Gene Expression Profiling / Q000662:veterinary*; D007413:Intestinal Mucosa / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D010053:Ovary / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013154:Spleen / Q000378:metabolism; D013950:Thymus Gland / Q000378:metabolism; D051193:Toll-Like Receptors / Q000235:genetics* / Q000378:metabolism
chemical_list: D012333:RNA, Messenger; D051193:Toll-Like Receptors