title: A facile method for preparation of tailored scaffolds for DNA-origami.
abstract: A convenient PCR cloning strategy allows one to prepare hundreds of picomoles of circular single-stranded DNA molecules, which are suitable as scaffolds for the assembly of DNA origami structures. The method is based on a combination of site-directed mutagenesis and site- and ligation-independent cloning protocols, with simultaneous insertion of a nicking endonuclease restriction site on a double-stranded plasmid of desired length and sequence.
authors.affiliation: Technische Universität Dortmund, Fakultät Chemie, Biologisch-Chemische Mikrostrukturtechnik, Otto Hahn Str. 6, 44227 Dortmund, Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Erkelenz
authors.forename: Dennis M
authors.initials: DM
authors.lastname: Bauer
authors.forename: Rebecca
authors.initials: R
authors.lastname: Meyer
authors.forename: Christos
authors.initials: C
authors.lastname: Gatsogiannis
authors.forename: Stefan
authors.initials: S
authors.lastname: Raunser
authors.forename: Barbara
authors.initials: B
authors.lastname: Saccà
authors.forename: Christof M
authors.initials: CM
authors.lastname: Niemeyer
mesh_terms: D004247:DNA / Q000737:chemistry*; D016297:Mutagenesis, Site-Directed; D016133:Polymerase Chain Reaction
chemical_list: D004247:DNA
keywords: DNA; bacterial cloning; nanostructures; self-assembly