title: A novel electroanalytical method for simultaneous detection of two neurotransmitter dopamine and serotonin in human serum.
abstract: BACKGROUND
  Simultaneous measurement of dopamine and serotonin levels is of greater value because of their coexistence in biological systems. These facts have encouraged chemists to develop faster, simpler, and more sensitive techniques to meet the various demands and many works have been published describing the measurement of serum dopamine and serotonin concentrations using chemically modified electrodes.
  
  
  METHODS
  Electrochemical techniques based on various chemically modified electrodes have been widely explored for the single determination of dopamine and serotonin in vivo or in vitro. To the best of our knowledge, there are only limit reports on the electrochemical determination of dopamine and serotonin using mesoporous materials. On the other hand, simultaneous measurement of dopamine and serotonin concentrations on the basis of silica based mesoporous materials has not been reported.
  
  
  RESULTS
  In the present work, the development an effective electroanalytical method for the simultaneous determination of dopamine and serotonin using cyclic and differential pulse voltammetry on the mobile crystalline material-41 functionalized by n-aminopropyl is described. Mobile crystalline material-41 functionalized by n-aminopropyl, a kind of mesoporous silica, was synthesized and then used to prepare an electrochemical device for simultaneous determination of dopamine and serotonin. A validated differential pulse voltammetry procedure was described to assay two neurotransmitters separately or combined in human serum.
  
  
  CONCLUSIONS
  The prepared sensor shows voltammetric responses with high sensitivity for dopamine and serotonin in optimal conditions, which makes it very suitable for simultaneous determination of these compounds.
authors.affiliation: Drug Applied Research Center, Tabriz University of Medical Sciences, P.O. Box 51664, Tabriz, Iran. mhmmd_hasanzadeh@yahoo.com
authors.forename: Mohammad
authors.initials: M
authors.lastname: Hasanzadeh
authors.forename: Nasrin
authors.initials: N
authors.lastname: Shadjou
authors.forename: Eskandar
authors.initials: E
authors.lastname: Omidinia
mesh_terms: D000328:Adult; D002384:Catalysis; D004298:Dopamine / Q000097:blood*; D055664:Electrochemical Techniques; D004563:Electrochemistry; D004566:Electrodes; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008297:Male; D010084:Oxidation-Reduction; D016062:Porosity; D011437:Propylamines / Q000737:chemistry*; D012701:Serotonin / Q000097:blood*; D012822:Silicon Dioxide / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D011437:Propylamines; D012701:Serotonin; D012822:Silicon Dioxide; D004298:Dopamine
keywords: Dopamine; Mesoporous; Neurotransmitter; Serotonin