title: Identification of nuclear retention domains in the RBM20 protein.
abstract: RBM20 is a nuclear protein which regulates alternative splicing of expressed genes that have a key role in cardiac function. By cloning the human and mouse RBM20 cDNA, producing expressing vectors for truncated proteins, and comparing their sub-cellular distribution in transfected cells, we have identified the sequences necessary for RBM20 full nuclear retention. The region overlaps an RNA binding motif and a serine-arginine domain. The sequence is conserved in many species but belongs only to RBM20 orthologs. The RMB20 tissue specificity, together with the properties of its nuclear localization determinant, demonstrates a specific evolutionary selection of post-transcriptional regulation factors.
authors.affiliation: Department of Life and Reproduction Sciences, Section of Biology and Genetics, University of Verona, Strada le Grazie 8, 37134 Verona, Italy. agnese.filippello@gmail.com
authors.forename: Agnese
authors.initials: A
authors.lastname: Filippello
authors.forename: Pamela
authors.initials: P
authors.lastname: Lorenzi
authors.forename: Elisa
authors.initials: E
authors.lastname: Bergamo
authors.forename: Maria Grazia
authors.initials: MG
authors.lastname: Romanelli
mesh_terms: D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D002467:Cell Nucleus / Q000235:genetics / Q000378:metabolism*; D003001:Cloning, Molecular; D017124:Conserved Sequence; D019143:Evolution, Molecular; D005786:Gene Expression Regulation; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D009928:Organ Specificity; D017434:Protein Structure, Tertiary; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
chemical_list: C584322:RBM20 protein, mouse; D016601:RNA-Binding Proteins; D011994:Recombinant Proteins; C543725:ribonucleic acid binding motif protein 20, human
keywords: Alternative splicing; BSA; Bp; DCM; GAPDH; GFP; NLS; NRS; Nuclear localization; PCR; PTB; RBM20; RBP; RNA binding protein; RRM; RT-PCR; Ribonucleoprotein; SR; base-pair(s); bovine serum albumin; cDNA; complementary DNA; dilatedcardiomyopathy; glyceraldehydes-3-phosphate dehydrogenase; green fluorescent protein; heterogenous nuclear ribonucleoproteins; hnRNP; nuclear localization signals; nuclear retention signal; polymerase chainreaction; polypyrimidine tract-binding protein; reverse transcription polymerase chain reaction; ribonucleic acid recognition motif; serine/arginine-rich protein