title: [Comparison of HER2 immunohistochemical results for advanced gastric cancer obtained on using 3 different antibodies].
abstract: We compared the results of immunohistochemical assessment of HER2 expression in 107 samples of advanced gastric cancer on using 3 currently used antibodies. Expression was scored as 0 to 3+, and equivocal or discordant cases were subjected to fluorescence in situ hybridization(FISH)analysis. HER2 scores of 2+or 3+were noted in 16.8% of cases(18/ 107)using SV2-61g, in 29.9% of cases(32/107)using Dako HercepTest, and in 34.6% of cases(37/107)using 4B5. The results of the HER2 test differed according to the antibodies used for immunohistochemistry preceding FISH analysis, and the HER2 positive rates after the FISH analysis were 14.0%(15/107)using SV2-61g, 19.6% (21/107)using Dako HercepTest, and 22.4% (24/107)using 4B5. Thus, therapeutic decisions might be considerably influenced by the antibody used for the HER2 test.
authors.affiliation: Dept. of Pathology and Laboratory Medicine, Yamagata Prefectural Central Hospital, Japan.
authors.forename: Mitsunobu
authors.initials: M
authors.lastname: Abe
authors.forename: Kohki
authors.initials: K
authors.lastname: Tsuchiya
authors.forename: Naoki
authors.initials: N
authors.lastname: Yanagawa
authors.forename: Shin-ya
authors.initials: SY
authors.lastname: Ogata
authors.forename: Takashi
authors.initials: T
authors.lastname: Nomura
authors.forename: Norimasa
authors.initials: N
authors.lastname: Fukushima
authors.forename: Gen
authors.initials: G
authors.lastname: Tamura
mesh_terms: D000906:Antibodies; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods*; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000032:analysis* / Q000276:immunology; D013274:Stomach Neoplasms / Q000737:chemistry* / Q000473:pathology
chemical_list: D000906:Antibodies; D018719:Erb-b2 Receptor Tyrosine Kinases; C508053:ERBB2 protein, human