title: [Simultaneous determination of twelve sweeteners and nine preservatives in foods by solid-phase extraction and LC-MS/MS].
abstract: A rapid and simple method for the simultaneous determination of twelve sweeteners and nine preservatives in various foods by liquid chromatography-tandem mass spectrometry (LC-MS/MS) was developed. The sweeteners and preservatives were extracted from solid samples with 80% and 50% methanol and from liquid samples with 80% methanol, followed by Oasis WAX cartridge cleanup. The LC separation was performed on a XSelect CSH Phenyl-Hexyl column (5 μm, 2.1 mm ×150 mm) with a mobile phase of 10 mmol/L acetate buffer (pH 4.0)-acetonitrile and MS detection with negative ion electrospray ionization. The quantification limits of acesulfame K (AK), alitame (AL), aspartame (ASP), cyclamic acid (CYC), neotame (NEO), saccharin Na (SAC), p-hydroxybenzoic acid methyl (PHBA-Me), p-hydroxybenzoic acid ethyl (PHBA-Et), p-hydroxybenzoic acid isopropyl (PHBA-iPr), p-hydroxybenzoic acid propyl (PHBA-Pr), p-hydroxybenzoic acid isobutyl (PHBA-iBu) and p-hydroxybenzoic acid butyl (PHBA-Bu) were 0.001 g/kg, those of dulcin (DU), glycyrrhizic acid (GLY), neohesperidin dihydrochalcone (NHDC), rebaudioside A (REB), stevioside (STV), sucralose (SUC) and benzoic acid (BA) were 0.005 g/kg, and those of sorbic acid (SOA) and dehydroacetic acid (DHA) were 0.02 g/kg. The mean recoveries from ten kinds of foods fortified at the levels of 0.02 and 0.2 g/kg were 70.9-119.0%, and their relative standard deviations were 0.1-11.7%.
authors.affiliation: Fukuoka City Institute for Hygiene and the Environment, 2-1-34 Jigyohama, Chuo-ku, Fukuoka 810-0065, Japan. tsuruda.s02@city.fukuoka.lg.jp
authors.forename: Sayuri
authors.initials: S
authors.lastname: Tsuruda
authors.forename: Tomonori
authors.initials: T
authors.lastname: Sakamoto
authors.forename: Kouichi
authors.initials: K
authors.lastname: Akaki
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods*; D005504:Food Analysis / Q000379:methods*; D005520:Food Preservatives / Q000302:isolation & purification*; D064427:Nutritive Sweeteners / Q000302:isolation & purification*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D005520:Food Preservatives; D064427:Nutritive Sweeteners