title: Knockdown of ECHS1 protein expression inhibits hepatocellular carcinoma cell proliferation via suppression of Akt activity.
abstract: Overexpression of ECHS1 occurs in different cancers, including hepatocellular carcinoma (HCC). ECHS1 is also reported to have an oncogenic activity in various human cancers. This study investigated the effect of ECHS1 knockdown on the regulation of HCC growth. ECHS1 shRNA suppressed the expression of ECHS1 protein in HepG2 cells compared to the negative control vector-transfected HCC cells. ECHS1 knockdown also reduced HCC cell viability and enhanced cisplatin-induced apoptosis in HCC cells. Akt activation and the expression of various cell cycle-related genes were inhibited following ECHS1 knockdown. ECHS1 shRNA suppressed hepatocellular carcinoma growth in tumor xenograft mice. These data demonstrate that ECHS1 may play a role in HCC progression, suggesting that inhibition of ECHS1 expression using ECHS1 shRNA should be further evaluated as a novel target for the control of HCC.
authors.affiliation: Department of Gastroenterology, the Chenggong Hospital Affiliated to Xiamen University, Wenyuan Road, Xiamen, Fujian 361003, China.
authors.forename: Xiao-san
authors.initials: XS
authors.lastname: Zhu
authors.forename: Yi-chen
authors.initials: YC
authors.lastname: Dai
authors.forename: Zhang-xing
authors.initials: ZX
authors.lastname: Chen
authors.forename: Jun-pei
authors.initials: JP
authors.lastname: Xie
authors.forename: Wei
authors.initials: W
authors.lastname: Zeng
authors.forename: Yuan-yuan
authors.initials: YY
authors.lastname: Lin
authors.forename: Qiu-hui
authors.initials: QH
authors.lastname: Tan
mesh_terms: D000818:Animals; D017209:Apoptosis; D006528:Carcinoma, Hepatocellular / Q000235:genetics; D002465:Cell Movement; D049109:Cell Proliferation; D002470:Cell Survival; D002478:Cells, Cultured; D002945:Cisplatin; D004746:Enoyl-CoA Hydratase / Q000235:genetics* / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic; D055785:Gene Knockdown Techniques; D056945:Hep G2 Cells; D006801:Humans; D020287:In Situ Nick-End Labeling; D008113:Liver Neoplasms / Q000235:genetics; D008297:Male; D051379:Mice; D008819:Mice, Nude; D051057:Proto-Oncogene Proteins c-akt / Q000037:antagonists & inhibitors* / Q000235:genetics* / Q000378:metabolism; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism
chemical_list: D034741:RNA, Small Interfering; D051057:Proto-Oncogene Proteins c-akt; D004746:Enoyl-CoA Hydratase; D002945:Cisplatin