title: Highly sensitive HPLC method for assay of aliskiren in human plasma through derivatization with 1-naphthyl isocyanate using UV detection.
abstract: A simple and sensitive HPLC method has been developed for the determination of aliskiren in human plasma through derivatization with 1-naphthyl isocyanate. The separation was achieved on a C18 column using a mobile phase consisting of acetonitrile/water/phosphoric acid (45:55:0.01, v/v/v, pH 3.2) in a flow rate of 1mL/min with UV detection at 230nm. Caffeine was used as an internal standard. The factors influencing the derivatization reaction yields were carefully studied and optimized. The method was linear over the concentration range of 5-400ng/mL with a detection limit of 0.5ng/mL and a limit of quantification of 1.0ng/mL. The relative standard deviation was less than 4.2% for both intra-day assay and inter-day assay results. No interferences from endogenous compounds were encountered. The percentage recovery was in the range 97.1-98.6%. The method is suitable for routine therapeutic drug monitoring and for pharmacokinetic studies.
authors.affiliation: Department of Analytical Chemistry, Faculty of Pharmacy, University of Mansoura, Mansoura 35516, Egypt. ffbelal@yahoo.com
authors.forename: F
authors.initials: F
authors.lastname: Belal
authors.forename: M
authors.initials: M
authors.lastname: Walash
authors.forename: N
authors.initials: N
authors.lastname: El-Enany
authors.forename: S
authors.initials: S
authors.lastname: Zayed
mesh_terms: D000577:Amides / Q000097:blood*; D000959:Antihypertensive Agents / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D016903:Drug Monitoring; D005650:Fumarates / Q000097:blood*; D006801:Humans; D017953:Isocyanates / Q000737:chemistry; D057230:Limit of Detection; D009281:Naphthalenes / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*
chemical_list: D000577:Amides; D000959:Antihypertensive Agents; D005650:Fumarates; D017953:Isocyanates; D009281:Naphthalenes; C446481:aliskiren; C061621:alpha-naphthyl isocyanate
keywords: Aliskiren; HPLC; Naphthyl isocyanate derivatization; Plasma