title: Development and characterization of a new cell line CF from caudal fin of knifefish, Chitala chitala (Hamilton-Buchanan).
abstract: A new cell line was successfully obtained from caudal fin tissue of the economically important freshwater fish Chitala chitala. The cell line was optimally maintained at 28°C in Leibovitz's L-15 supplemented with 20% fetal bovine serum (FBS). The effects of temperature and concentration of FBS on the growth of CF cells were examined. The CF cell line consisted predominantly of fibroblastic-like cells. Moderately low plating efficiencies 8%, 11%, and 17% were observed, with CF cell line in L-15 Medium with 20% FBS. Chromosomal analysis of the cell line revealed a diploid number of 42 chromosomes in C. chitala. Molecular characterization of mitochondrial 16S rRNA and cytochrome oxidase subunit I confirmed the origin of the cell line. The cells were successfully cryopreserved in liquid nitrogen (-196°C) for 6 mo, and more than 85-90% of CF cells were revived.
authors.affiliation: National Bureau of Fish Genetic Resources, Lucknow, Uttar Pradesh, India, smitakapoor08@gmail.com.
authors.forename: Smita
authors.initials: S
authors.lastname: Kapoor
authors.forename: J P
authors.initials: JP
authors.lastname: Bhatt
authors.forename: W S
authors.initials: WS
authors.lastname: Lakra
mesh_terms: D058500:Animal Fins / Q000166:cytology*; D000818:Animals; D018929:Cell Culture Techniques; D002460:Cell Line; D015925:Cryopreservation; D003576:Electron Transport Complex IV; D005347:Fibroblasts / Q000166:cytology*; D005399:Fishes / Q000235:genetics* / Q000254:growth & development; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D013696:Temperature
chemical_list: D012336:RNA, Ribosomal, 16S; D003576:Electron Transport Complex IV