title: Pluripotent stem cells induced from mouse somatic cells by small-molecule compounds.
abstract: Pluripotent stem cells can be induced from somatic cells, providing an unlimited cell resource, with potential for studying disease and use in regenerative medicine. However, genetic manipulation and technically challenging strategies such as nuclear transfer used in reprogramming limit their clinical applications. Here, we show that pluripotent stem cells can be generated from mouse somatic cells at a frequency up to 0.2% using a combination of seven small-molecule compounds. The chemically induced pluripotent stem cells resemble embryonic stem cells in terms of their gene expression profiles, epigenetic status, and potential for differentiation and germline transmission. By using small molecules, exogenous "master genes" are dispensable for cell fate reprogramming. This chemical reprogramming strategy has potential use in generating functional desirable cell types for clinical applications.
authors.affiliation: College of Life Sciences and Peking-Tsinghua Center for Life Sciences, Peking University, Beijing 100871, China.
authors.forename: Pingping
authors.initials: P
authors.lastname: Hou
authors.forename: Yanqin
authors.initials: Y
authors.lastname: Li
authors.forename: Xu
authors.initials: X
authors.lastname: Zhang
authors.forename: Chun
authors.initials: C
authors.lastname: Liu
authors.forename: Jingyang
authors.initials: J
authors.lastname: Guan
authors.forename: Honggang
authors.initials: H
authors.lastname: Li
authors.forename: Ting
authors.initials: T
authors.lastname: Zhao
authors.forename: Junqing
authors.initials: J
authors.lastname: Ye
authors.forename: Weifeng
authors.initials: W
authors.lastname: Yang
authors.forename: Kang
authors.initials: K
authors.lastname: Liu
authors.forename: Jian
authors.initials: J
authors.lastname: Ge
authors.forename: Jun
authors.initials: J
authors.lastname: Xu
authors.forename: Qiang
authors.initials: Q
authors.lastname: Zhang
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhao
authors.forename: Hongkui
authors.initials: H
authors.lastname: Deng
mesh_terms: D000818:Animals; D015820:Cadherins / Q000235:genetics; D060846:Cell Engineering / Q000379:methods*; D065150:Cellular Reprogramming / Q000187:drug effects* / Q000235:genetics; D058750:Epithelial-Mesenchymal Transition / Q000187:drug effects / Q000235:genetics; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects*; D020869:Gene Expression Profiling; D049452:Green Fluorescent Proteins / Q000235:genetics; D057026:Induced Pluripotent Stem Cells / Q000166:cytology* / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D008813:Mice, Inbred ICR; D050814:Octamer Transcription Factor-3 / Q000235:genetics / Q000378:metabolism; D011401:Promoter Regions, Genetic / Q000187:drug effects; D054852:Small Molecule Libraries / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D015820:Cadherins; D050814:Octamer Transcription Factor-3; C494174:Pou5f1 protein, mouse; D054852:Small Molecule Libraries; D049452:Green Fluorescent Proteins