title: cDNA cloning and structural characterization of a lectin from the mussel Crenomytilus grayanus with a unique amino acid sequence and antibacterial activity.
abstract: An amino acid sequence of GalNAc/Gal-specific lectin from the mussel Crenomytilus grayanus (CGL) was determined by cDNA sequencing. CGL consists of 150 amino acid residues, contains three tandem repeats with high sequence similarities to each other (up to 73%) and does not belong to any known lectins family. According to circular dichroism results CGL is a β/α-protein with the predominance of β-structure. CGL was predicted to adopt a ß-trefoil fold. The lectin exhibits antibacterial activity and might be involved in the recognition and clearance of bacterial pathogens in the shellfish.
authors.affiliation: G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Science, Prospect 100 let Vladivostoku 159, Vladivostok 690022, Russian Federation. s.n.kovalchuk@gmail.com
authors.forename: Svetlana N
authors.initials: SN
authors.lastname: Kovalchuk
authors.forename: Irina V
authors.initials: IV
authors.lastname: Chikalovets
authors.forename: Oleg V
authors.initials: OV
authors.lastname: Chernikov
authors.forename: Valentina I
authors.initials: VI
authors.lastname: Molchanova
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.forename: Valery A
authors.initials: VA
authors.lastname: Rasskazov
authors.forename: Pavel A
authors.initials: PA
authors.lastname: Lukyanov
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000378:metabolism; D001419:Bacteria / Q000187:drug effects / Q000254:growth & development; D001483:Base Sequence; D002942:Circular Dichroism; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics / Q000378:metabolism; D037102:Lectins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D049879:Mytilidae / Q000235:genetics* / Q000378:metabolism / Q000382:microbiology; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment
chemical_list: D000900:Anti-Bacterial Agents; D018076:DNA, Complementary; D037102:Lectins; D012333:RNA, Messenger
keywords: Amino acid sequence; Antibacterial activity; Crenomytilus grayanus; GalNAc/Gal-specific lectin; ß-trefoil fold