title: The influence of cerebrospinal fluid on epidermal neural crest stem cells may pave the path for cell-based therapy.
abstract: BACKGROUND
  Epidermal neural crest stem cells (EPI-NCSCs) in the bulge of hair follicles are a promising source for cell-replacement therapies in neurodegenerative diseases. A prominent factor in cell-based therapy is the practicalities of different routes of administration. Cerebrospinal fluid (CSF), owing to its adaptive library of secreted growth factors, can provide a trophic environment for transplanted cells. Thus, the effect of CSF on the behavior of EPI-NCSC was studied here.
  
  
  METHODS
  In this study, the highly pure population of EPI-NCSCs was obtained from the bulge of mouse hair follicle. Migrated cells were characterized with real-time polymerase chain reaction (RT-PCR) and immunocytochemistry. Subsequently isolated stem cells were cultured in CSF, which was collected from the cisterna magna of the adult rat. The expression of pertinent markers was assessed at the gene and protein levels with RT-PCR and immunocytochemistry, respectively. Colorimetric immunoassay was used to quantify the rate of proliferation of EPI-NCSCs after cultivation in CSF.
  
  
  RESULTS
  Isolated EPI-NCSCs could survive in the CSF, and they maintained the expression of nestin, β-tubulin ІІІ (early neuronal marker), and glial fibrillary acidic protein (GFAP, glia marker) in this environment. In addition, CSF decreased the proliferation rate of EPI-NCSCs significantly in comparison to primary and expansion culture medium.
  
  
  CONCLUSIONS
  Our findings demonstrate that CSF as a cocktail of growth factors helps EPI-NCSCs to acquire some desirable traits, and because of its circulatory system that is in close contact with different parts of the central nervous system (CNS), can be a practical route of administration for delivery of injected stem cells.
authors.forename: Sareh
authors.initials: S
authors.lastname: Pandamooz
authors.forename: Mohammad
authors.initials: M
authors.lastname: Naji
authors.forename: Farid
authors.initials: F
authors.lastname: Alinezhad
authors.forename: Amin
authors.initials: A
authors.lastname: Zarghami
authors.forename: Mohsen
authors.initials: M
authors.lastname: Pourghasem
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002555:Cerebrospinal Fluid / Q000378:metabolism*; D002642:Chick Embryo; D051379:Mice; D009432:Neural Crest / Q000166:cytology / Q000378:metabolism*; D058953:Neural Stem Cells / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism*