title: Complete sequence analysis of two cryptic plasmids from Bifidobacterium kashiwanohense JCM 15439 (type strain) isolated from healthy infant feces.
abstract: Bifidobacterial plasmids reported so far are derived from a limited number of strains and plasmids of bifidobacterial type strains isolated from humans are unknown. We found that Bifidobacterium kashiwanohense JCM 15439 (type strain) isolated from a healthy infant contained two cryptic plasmids, designated pBBKW-1 and pBBKW-2. We determined and analyzed the complete sequences of both plasmids. pBBKW-1 (7716 bp) was predicted to replicate by a rolling-circle mechanism and encode six protein-coding genes, two of which are putative replication proteins. pBBKW-1 seems to be a cointegrate plasmid containing two copies of the plasmid pMG1 from Bifidobacterium longum. pBBKW-2 (2920 bp) was predicted to encode six protein-coding genes and be a theta-type replicating plasmid, which has been reported to be more stable than a rolling circle-type replicating plasmid frequently found in bifidobacteria. Our finding will provide new insights into safe recombinant plasmid constructions for humans.
authors.affiliation: School of Veterinary Medicine, Azabu University, Sagamihara; BioBank Co., Ltd, Okayama.
authors.forename: Muneaki
authors.initials: M
authors.lastname: Takahata
authors.forename: Hidehiro
authors.initials: H
authors.lastname: Toh
authors.forename: Akiyo
authors.initials: A
authors.lastname: Nakano
authors.forename: Misako
authors.initials: M
authors.lastname: Takagi
authors.forename: Masaru
authors.initials: M
authors.lastname: Murakami
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Ishii
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Takizawa
authors.forename: Soichi
authors.initials: S
authors.lastname: Tanabe
authors.forename: Hidetoshi
authors.initials: H
authors.lastname: Morita
mesh_terms: D001483:Base Sequence; D001644:Bifidobacterium / Q000235:genetics* / Q000302:isolation & purification; D005243:Feces / Q000382:microbiology*; D006801:Humans; D007223:Infant; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics*; D017422:Sequence Analysis, DNA
keywords: Bifidobacterium; human origin; plasmid