title: Expression of three novel cytochrome P450 (CYP) and antioxidative genes from the polychaete, Perinereis nuntia exposed to water accommodated fraction (WAF) of Iranian crude oil and benzo[a]pyrene.
abstract: To report a novel CYP genes and to evaluate its potency as a biomarker for oil pollution, we cloned three CYP genes and measured their expression profiles under controlled lab conditions using real-time reverse transcription PCR (real-time RT-PCR) after exposure of the water accommodated fraction (WAF) of Iranian crude oil and benzo[α]pyrene (B[α]P) as a positive control. Of these, CYP432A1 (CYP3 clan) gene was significantly induced by B[α]P exposure, indicating that the CYP3 clan gene would play an important role in polycyclic aromatic hydrocarbon (PAH) metabolisms, particularly for B[α]P in this species. However, the Perinereis nuntia CYP431A1 mRNA, a CYP2 clan gene, was sensitively expressed to WAF exposure with other two CYP genes. As one of Phase II detoxification enzymes, the glutathione S-transferase (GST) genes also upregulated with other antioxidant genes (SOD and CAT), indicating that WAF-exposed P. nuntia was properly responding to this kind of chemical stress. Thus, three CYP genes from the polychaete, P. nuntia have a potential as a biomarker in monitoring of the marine sediment after an oil spill accident.
authors.affiliation: Research Institute for Natural Sciences, Hanyang University, Seoul 133-791, South Korea.
authors.forename: Eun-Ji
authors.initials: EJ
authors.lastname: Won
authors.forename: Jae-Sung
authors.initials: JS
authors.lastname: Rhee
authors.forename: Kyung-Hoon
authors.initials: KH
authors.lastname: Shin
authors.forename: Jee-Hyun
authors.initials: JH
authors.lastname: Jung
authors.forename: Won Joon
authors.initials: WJ
authors.lastname: Shim
authors.forename: Young-Mi
authors.initials: YM
authors.lastname: Lee
authors.forename: Jae-Seong
authors.initials: JS
authors.lastname: Lee
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001564:Benzo(a)pyrene / Q000633:toxicity*; D015415:Biomarkers / Q000378:metabolism; D003577:Cytochrome P-450 Enzyme System / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D004781:Environmental Exposure; D004784:Environmental Monitoring; D015971:Gene Expression Regulation, Enzymologic; D008969:Molecular Sequence Data; D010578:Petroleum / Q000633:toxicity*; D010802:Phylogeny; D011077:Polychaeta / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D016133:Polymerase Chain Reaction; D016415:Sequence Alignment; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D015415:Biomarkers; D010578:Petroleum; D014874:Water Pollutants, Chemical; D001564:Benzo(a)pyrene; D003577:Cytochrome P-450 Enzyme System
keywords: Antioxidant genes; Benzo[α]pyrene; Cytochrome P450; Perinereis nuntia; Polychaete; WAF; Water accommodated fraction