title: Preparation and characterization of silver-doped nanobioactive glass particles and their in vitro behaviour for biomedical applications.
abstract: In this study, silver-doped silica- and phosphate-based nanobioactive glass compositions (58SiO2-(33- x)CaO-9P2O5-xAg2O) (x = 0, 0.5, 1, 2 and 3 mol%) were synthesised by a simple and cost-effective sol-gel method. The prepared samples were characterised by X-ray diffraction, Fourier transform infrared spectroscopy, transmission electron microscopy, scanning electron microscopy and energy-dispersive X-ray fluorescence spectrometer studies. All the compositions of the glass samples revealed amorphous phase with spherical morphology and a particle size less than 100 nm. The prepared glass samples reveal the specific surface area in the range of 55.31-90.69 m2 g(-1). The bioactivity of glass samples was confirmed through the formation of the hydroxyapatite layer on glass surfaces during in vitro studies in which silver doped glasses (2 and 3 mol%) showed better bioactivity. A better biocompatibility was achieved in human gastric adenocarcinoma cell line in case of silver-free glass sample while comparing the biological behaviour of Ag2O-doped glasses. Further, the Ag2O-doped nanobioactive glasses revealed significant antibacterial activity against Staphylococcus aureus and Escherichia coli. Ag2O substitutions showed better in vitro bioactivity and remained slightly toxic to human cells at a concentration of 100 microg mL(-1). Silver-doped nanobioactive glass shows good antimicrobial property as well as no significant toxic for implant applications.
authors.affiliation: Centre for Nanoscience and Technology, K. S. Rangasamy College of Technology, Tiruchengode 637215, Namakkal (Dt), Tamil Nadu, India.
authors.forename: Muthusamy
authors.initials: M
authors.lastname: Prabhu
authors.forename: Kandiah
authors.initials: K
authors.lastname: Kavitha
authors.forename: Rangaraj
authors.initials: R
authors.lastname: Suriyaprabha
authors.forename: Palanisamy
authors.initials: P
authors.lastname: Manivasakan
authors.forename: Venkatachalam
authors.initials: V
authors.lastname: Rajendran
authors.forename: Palanisami
authors.initials: P
authors.lastname: Kulandaivelu
mesh_terms: D000230:Adenocarcinoma / Q000473:pathology; D000327:Adsorption; D000890:Anti-Infective Agents / Q000494:pharmacology; D001672:Biocompatible Materials / Q000737:chemistry; D001709:Biotechnology / Q000379:methods*; D045744:Cell Line, Tumor; D004926:Escherichia coli / Q000187:drug effects; D005898:Glass; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D036103:Nanotechnology / Q000379:methods*; D044367:Phase Transition; D011312:Pressure; D012834:Silver / Q000737:chemistry*; D013211:Staphylococcus aureus / Q000187:drug effects; D014961:X-Ray Diffraction
chemical_list: D000890:Anti-Infective Agents; D001672:Biocompatible Materials; D012834:Silver