title: [Experimental study on hemostasis of thermosensitive chitosan hemostatic film].
abstract: OBJECTIVE
  To investigate the hemostasis of thermosensitive chitosan hemostatic film.
  
  
  METHODS
  Fifty adult Sprague Dawley rats, male or female and weighing 190-210 g, were made the models of liver injury. The models were randomly divided into 5 groups (n=10) depending on different hemostatic materials. The incision of the liver was covered with the hemostatic materials of 2.0 cm x 1.0 cm x 0.5 cm in size: thermosensitive chitosan hemostatic film (group A), chitosan hemostatic film (group B), cellulose hemostatic cotton (group C), gelatin sponge (group D), and no treatment (group E), respectively. The bleeding time and bleeding amount were recorded. After 4 weeks, the incisions of the liver were observed with HE staining.
  
  
  RESULTS
  Gross observation showed better hemostatic effect and faster hemostatic time in groups A, B, and C; group D had weaker hemostatic effect and slower hemostatic time; group E had no hemostatic effect. The bleeding time and bleeding amount of groups A, B, C, and D were significantly lower than those of group E (P < 0.05). The bleeding time and bleeding amount of groups A, B, and C were significantly lower than those of group D (P < 0.05), but no significant difference was found among groups A, B, and C (P > 0.05). The liver cells of group A had milder edema and ballooning degeneration than other 4 groups through histological observation.
  
  
  CONCLUSIONS
  The thermosensitive chitosan hemostatic film has good hemostasis effect on the liver incision of rats.
authors.affiliation: Department of Orthopaedics, Shanghai Changzheng Hospital, Shanghai, 200003, PR China.
authors.forename: Gang
authors.initials: G
authors.lastname: Yin
authors.forename: Changzheng
authors.initials: C
authors.lastname: Wei
authors.forename: Xingfeng
authors.initials: X
authors.lastname: Guo
authors.forename: Huihao
authors.initials: H
authors.lastname: Chen
authors.forename: Chunlin
authors.initials: C
authors.lastname: Hou
mesh_terms: D000818:Animals; D002482:Cellulose / Q000008:administration & dosage / Q000627:therapeutic use; D048271:Chitosan / Q000008:administration & dosage / Q000627:therapeutic use*; D004195:Disease Models, Animal; D005260:Female; D006470:Hemorrhage / Q000628:therapy*; D006487:Hemostasis / Q000187:drug effects; D006490:Hemostatics / Q000008:administration & dosage / Q000627:therapeutic use*; D008099:Liver / Q000293:injuries*; D008297:Male; D008422:Materials Testing / Q000379:methods; D008567:Membranes, Artificial; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D013696:Temperature
chemical_list: D006490:Hemostatics; D008567:Membranes, Artificial; D002482:Cellulose; D048271:Chitosan