title: Quality assessment of buffy-coat-derived leucodepleted platelet concentrates in PAS-plasma, prepared by the OrbiSac or TACSI automated system.
abstract: OBJECTIVE
  Buffy-coat (BC)-derived platelet concentrates (PCs) are the predominant product for platelet transfusion in many countries. Two automated systems, OrbiSac and TACSI, have been introduced in blood centres to prepare these PCs, as an alternative to the manual method. We compared the in vitro quality of PCs prepared by both methods during standard storage.
  
  
  METHODS
  Twenty primary BC pools were split into two parts, which were processed with OrbiSac and TACSI system to obtain OrbiSac PCs (O-PCs) and TACSI PCs (T-PCs), respectively. On days 1, 5 and 7 of standard storage, samples were taken and the following analysed: cell count, metabolic variables, platelet function and content of activation and proinflammatory substances.
  
  
  RESULTS
  Both the OrbiSac and TACSI systems produced PCs that meet the standards for platelet products in terms of platelet and leucocyte content. In vitro evaluation pointed to the similar preservation of platelet metabolism (pH, glucose, bicarbonate and lactate) in O-PCs and T-PCs. Moreover, there were no significant differences between O-PCs and T-PCs as regards the hypotonic shock response or in the platelet aggregation profile. The OrbiSac system caused greater platelet activation, which resulted in higher concentrations of sCD62P, RANTES and sCD40L on the day the PCs were prepared.
  
  
  CONCLUSIONS
  The systems OrbiSac and TACSI can be used to produce buffy-coat-derived PCs whose cell content, platelet function and metabolism are similar during standard storage. However, the preparation with the OrbiSac system induces a transient increase in platelet activation and release of proinflammatory substances.
authors.affiliation: Centro Regional de Hemodonación, Servicio de Hematología y Oncología Médica, Hospital Universitario Morales Meseguer Universidad de Murcia, Murcia, Spain.
authors.forename: E M
authors.initials: EM
authors.lastname: Plaza
authors.forename: P
authors.initials: P
authors.lastname: Céspedes
authors.forename: H
authors.initials: H
authors.lastname: Fernández
authors.forename: M I
authors.initials: MI
authors.lastname: Sánchez-Guiu
authors.forename: J M
authors.initials: JM
authors.lastname: Egea
authors.forename: V
authors.initials: V
authors.lastname: Vicente
authors.forename: M L
authors.initials: ML
authors.lastname: Lozano
authors.forename: J
authors.initials: J
authors.lastname: Rivera
mesh_terms: D058621:Blood Buffy Coat / Q000166:cytology* / Q000502:physiology; D001792:Blood Platelets / Q000166:cytology* / Q000502:physiology; D006801:Humans; D047589:Leukocyte Reduction Procedures; D010949:Plasma / Q000737:chemistry / Q000166:cytology*; D015539:Platelet Activation; D010974:Platelet Aggregation; D010979:Platelet Function Tests; D017713:Platelet Transfusion; D010983:Plateletpheresis / Q000295:instrumentation*
keywords: ORBISAC; RANTES; TACSI; platelet concentrates; sCD62P; sCL40L