title: Expression of cytochrome P450 2A5 in a C57BL/6J mouse model of nonalcoholic fatty liver disease.
abstract: Cytochrome P450 2A5 (CYP2A5) expression is induced during the hepatotoxicity caused by various hepatotoxins and hepatitis. However, CYP2A5 expression during nonalcoholic fatty liver disease (NAFLD) is still unknown. In this study, serum biochemical parameters and liver histopathological analyses found that NAFLD had developed in C57BL/6J mice via administration of a high-fat diet continuously for 8 weeks. Subsequently, CYP2A5 expression was probed in the mice diagnosed with NAFLD that were treated with or without pyrazole, the inducer of chemical liver injury. It is shown that hepatic CYP2A5 mRNA, protein expression and coumarin 7-hydroxylase activity are enhanced with high-fat feed, and that pyrazole is able to further increase CYP2A5 expression and activity in mice with NAFLD. These results revealed that CYP2A5 is elevated during NAFLD and suggested that pyrazole and NAFLD act synergistically to induce the expression of CYP2A5 via an unclear mechanism.
authors.affiliation: College of Veterinary Medicine, Northeast Agricultural University, Harbin, PR China.
authors.forename: Rui
authors.initials: R
authors.lastname: Li
authors.forename: Guifang
authors.initials: G
authors.lastname: Guo
authors.forename: Yuzhen
authors.initials: Y
authors.lastname: Cao
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Wang
authors.forename: Fangping
authors.initials: F
authors.lastname: Liu
authors.forename: Xiuying
authors.initials: X
authors.lastname: Zhang
mesh_terms: D000818:Animals; D001189:Aryl Hydrocarbon Hydroxylases / Q000096:biosynthesis* / Q000235:genetics; D065637:Cytochrome P-450 CYP2A6; D000072467:Cytochrome P450 Family 2; D059305:Diet, High-Fat; D004195:Disease Models, Animal; D020536:Enzyme Activators / Q000494:pharmacology*; D004790:Enzyme Induction; D005234:Fatty Liver / Q000201:enzymology* / Q000473:pathology; D008099:Liver / Q000201:enzymology / Q000473:pathology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D065626:Non-alcoholic Fatty Liver Disease; D011720:Pyrazoles / Q000494:pharmacology*; D012333:RNA, Messenger / Q000096:biosynthesis
chemical_list: D020536:Enzyme Activators; D011720:Pyrazoles; D012333:RNA, Messenger; C031280:pyrazole; D001189:Aryl Hydrocarbon Hydroxylases; C585520:Cyp2a5 protein, mouse; D065637:Cytochrome P-450 CYP2A6; D000072467:Cytochrome P450 Family 2