title: Detection of antibodies to the 20s proteasome by ELISA.
abstract: The presence of antibodies against the 20S proteasome has been correlated with diseases like multiple sclerosis (MS) and systemic lupus erythematosus (SLE) but no definite association has been established. In order to investigate this further, we optimized an ELISA for proteasome antibodies and applied it to test a total of 324 serum and plasma samples from MS patients, SLE patients, and healthy controls. Our results yield a functional and reliable assay but no correlation between the amount of proteasome antibodies present and the development of MS or SLE could be established.
authors.affiliation: Department of International Health, Immunology and Microbiology, University of Copenhagen, Copenhagen, Denmark.
authors.forename: Karin Meinike
authors.initials: KM
authors.lastname: Jørgensen
authors.forename: Jette Lautrup
authors.initials: JL
authors.lastname: Frederiksen
authors.forename: Christoffer Tandrup
authors.initials: CT
authors.lastname: Nielsen
authors.forename: Gunnar
authors.initials: G
authors.lastname: Houen
mesh_terms: D000328:Adult; D000368:Aged; D001323:Autoantibodies / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005260:Female; D064368:Healthy Volunteers; D006801:Humans; D008180:Lupus Erythematosus, Systemic / Q000276:immunology; D008297:Male; D008875:Middle Aged; D009103:Multiple Sclerosis / Q000276:immunology; D009902:Optic Neuritis / Q000276:immunology; D046988:Proteasome Endopeptidase Complex / Q000276:immunology*
chemical_list: D001323:Autoantibodies; D046988:Proteasome Endopeptidase Complex