title: Coordination of heart and lung co-development by a multipotent cardiopulmonary progenitor.
abstract: Co-development of the cardiovascular and pulmonary systems is a recent evolutionary adaption to terrestrial life that couples cardiac output with the gas exchange function of the lung. Here we show that the murine pulmonary vasculature develops even in the absence of lung development. We have identified a population of multipotent cardiopulmonary mesoderm progenitors (CPPs) within the posterior pole of the heart that are marked by the expression of Wnt2, Gli1 and Isl1. We show that CPPs arise from cardiac progenitors before lung development. Lineage tracing and clonal analysis demonstrates that CPPs generate the mesoderm lineages within the cardiac inflow tract and lung including cardiomyocytes, pulmonary vascular and airway smooth muscle, proximal vascular endothelium, and pericyte-like cells. CPPs are regulated by hedgehog expression from the foregut endoderm, which is required for connection of the pulmonary vasculature to the heart. Together, these studies identify a novel population of multipotent cardiopulmonary progenitors that coordinates heart and lung co-development that is required for adaptation to terrestrial existence.
authors.affiliation: Department of Medicine, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA.
authors.forename: Tien
authors.initials: T
authors.lastname: Peng
authors.forename: Ying
authors.initials: Y
authors.lastname: Tian
authors.forename: Cornelis J
authors.initials: CJ
authors.lastname: Boogerd
authors.forename: Min Min
authors.initials: MM
authors.lastname: Lu
authors.forename: Rachel S
authors.initials: RS
authors.lastname: Kadzik
authors.forename: Kathleen M
authors.initials: KM
authors.lastname: Stewart
authors.forename: Sylvia M
authors.initials: SM
authors.lastname: Evans
authors.forename: Edward E
authors.initials: EE
authors.lastname: Morrisey
mesh_terms: D000818:Animals; D002302:Cardiac Output; D019070:Cell Lineage; D004707:Endoderm / Q000378:metabolism; D006321:Heart / Q000033:anatomy & histology / Q000196:embryology*; D053823:Hedgehog Proteins / Q000378:metabolism; D051741:Kruppel-Like Transcription Factors / Q000378:metabolism; D060850:LIM-Homeodomain Proteins / Q000378:metabolism; D008168:Lung / Q000098:blood supply / Q000166:cytology* / Q000196:embryology*; D008648:Mesoderm / Q000166:cytology; D051379:Mice; D023421:Models, Animal; D039902:Multipotent Stem Cells / Q000166:cytology*; D032386:Myoblasts, Cardiac / Q000166:cytology*; D038081:Organogenesis; D020286:Pericytes / Q000166:cytology; D011659:Pulmonary Gas Exchange; D014157:Transcription Factors / Q000378:metabolism; D051153:Wnt Proteins / Q000378:metabolism; D000071676:Zinc Finger Protein GLI1
chemical_list: C498520:Gli1 protein, mouse; D053823:Hedgehog Proteins; D051741:Kruppel-Like Transcription Factors; D060850:LIM-Homeodomain Proteins; D014157:Transcription Factors; D051153:Wnt Proteins; D000071676:Zinc Finger Protein GLI1; C063843:insulin gene enhancer binding protein Isl-1