title: Nanosensor dosimetry of mouse blood proteins after exposure to ionizing radiation.
abstract: Giant magnetoresistive (GMR) nanosensors provide a novel approach for measuring protein concentrations in blood for medical diagnosis. Using an in vivo mouse radiation model, we developed protocols for measuring Flt3 ligand (Flt3lg) and serum amyloid A1 (Saa1) in small amounts of blood collected during the first week after X-ray exposures of sham, 0.1, 1, 2, 3, or 6 Gy. Flt3lg concentrations showed excellent dose discrimination at ≥ 1 Gy in the time window of 1 to 7 days after exposure except 1 Gy at day 7. Saa1 dose response was limited to the first two days after exposure. A multiplex assay with both proteins showed improved dose classification accuracy. Our magneto-nanosensor assay demonstrates the dose and time responses, low-dose sensitivity, small volume requirements, and rapid speed that have important advantages in radiation triage biodosimetry.
authors.affiliation: Department of Materials Science and Engineering, Stanford University, Stanford, California 94305, USA.
authors.forename: Dokyoon
authors.initials: D
authors.lastname: Kim
authors.forename: Francesco
authors.initials: F
authors.lastname: Marchetti
authors.forename: Zuxiong
authors.initials: Z
authors.lastname: Chen
authors.forename: Sasa
authors.initials: S
authors.lastname: Zaric
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Wilson
authors.forename: Drew A
authors.initials: DA
authors.lastname: Hall
authors.forename: Richard S
authors.initials: RS
authors.lastname: Gaster
authors.forename: Jung-Rok
authors.initials: JR
authors.lastname: Lee
authors.forename: Junyi
authors.initials: J
authors.lastname: Wang
authors.forename: Sebastian J
authors.initials: SJ
authors.lastname: Osterfeld
authors.forename: Heng
authors.initials: H
authors.lastname: Yu
authors.forename: Robert M
authors.initials: RM
authors.lastname: White
authors.forename: William F
authors.initials: WF
authors.lastname: Blakely
authors.forename: Leif E
authors.initials: LE
authors.lastname: Peterson
authors.forename: Sandhya
authors.initials: S
authors.lastname: Bhatnagar
authors.forename: Brandon
authors.initials: B
authors.lastname: Mannion
authors.forename: Serena
authors.initials: S
authors.lastname: Tseng
authors.forename: Kristen
authors.initials: K
authors.lastname: Roth
authors.forename: Matthew
authors.initials: M
authors.lastname: Coleman
authors.forename: Antoine M
authors.initials: AM
authors.lastname: Snijders
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Wyrobek
authors.forename: Shan X
authors.initials: SX
authors.lastname: Wang
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000097:blood; D015374:Biosensing Techniques / Q000295:instrumentation / Q000592:standards; D001798:Blood Proteins; D004307:Dose-Response Relationship, Radiation; D005260:Female; D008297:Male; D008565:Membrane Proteins / Q000097:blood; D051379:Mice; D036103:Nanotechnology; D011839:Radiation, Ionizing; D011874:Radiometry; D015203:Reproducibility of Results; D000685:Serum Amyloid A Protein; D013997:Time Factors
chemical_list: D015415:Biomarkers; D001798:Blood Proteins; D008565:Membrane Proteins; C489995:Saa2 protein, mouse; D000685:Serum Amyloid A Protein; C084881:flt3 ligand protein