title: An intrinsic therapy of gold nanoparticles in focal cerebral ischemia-reperfusion injury in rats.
abstract: Ischemic stroke is a leading cause of death and disability, and the treatment options are limited. Recent studies demonstrated that the promising applications of gold nanoparticles (Au-NPs) as intrinsic therapeutics; however, little is known about the effect of Au-NPs on cerebral ischemia-reperfusion (I/R) injury. In this paper, the role of Au-NPs on cerebral I/R injury in the rat middle cerebral artery occlusion (MCAO) model was investigated using citrate-capped 5 nm and 20 nm Au-NPs. It was found that 20 nm Au-NPs administration led to remarkable amelioration of neurologic deficits and infarction volumes. Further research revealed that 20 nm Au-NPs elevated the production of anti-inflammatory cytokines, as well as inhibited I/R induced activation of astrocytes and microglias. Moreover, the expressions of anti-apoptotic proteins were up-regulated and the pro-apoptotic molecules were down-regulated in the post-ischemic brains. These effects were opposite in 5 nm group. The above results showed that the neuroprotection of 20 nm Au-NPs was attributable to its anti-inflammatory and anti-apoptotic effect. This study could provide a novel strategy for the treatment of cerebral I/R injury.
authors.affiliation: Department of Geriatrics, the Second Affiliated Hospital, Nanjing Medical University, Jiangsu 210029, China.
authors.forename: Zhengxia
authors.initials: Z
authors.lastname: Liu
authors.forename: Yujie
authors.initials: Y
authors.lastname: Shen
authors.forename: Yucheng
authors.initials: Y
authors.lastname: Wu
authors.forename: Yujiao
authors.initials: Y
authors.lastname: Yang
authors.forename: Jin
authors.initials: J
authors.lastname: Wu
authors.forename: Ping
authors.initials: P
authors.lastname: Zhou
authors.forename: Xiang
authors.initials: X
authors.lastname: Lu
authors.forename: Zhirui
authors.initials: Z
authors.lastname: Guo
mesh_terms: D000818:Animals; D002545:Brain Ischemia / Q000188:drug therapy* / Q000473:pathology / Q000503:physiopathology*; D006046:Gold / Q000737:chemistry* / Q000627:therapeutic use*; D008297:Male; D008422:Materials Testing; D053758:Nanoparticles / Q000737:chemistry* / Q000627:therapeutic use*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000188:drug therapy* / Q000473:pathology / Q000503:physiopathology*; D016896:Treatment Outcome
chemical_list: D006046:Gold