title: Steroid receptor coactivator-3 promotes bladder cancer through upregulation of CXCR4.
abstract: The three homologous members of the p160 SRC family (SRC-1, SRC-2 and SRC-3) mediate the transcriptional functions of nuclear receptors and other transcription factors, and are the most studied of all the transcriptional co-activators. Recent work has indicated that the SRC-3 gene is subject to amplification and overexpression in various human cancers. Some of the molecular mechanisms responsible for SRC overexpression, along with the mechanisms by which SRC-3 promotes breast and prostate cancer cell proliferation and survival, have been identified. However, the function of SRC-3 in bladder cancer remains poorly understood. In the present study, our results indicate that overexpression of SRC-3 promotes bladder cancer cell proliferation whereas knockdown of SRC-3 results in inhibition. At the molecular level, we further established that CXCR4 is a transcriptional target of SRC-3. Therefore, our study first identified that SRC-3 plays a critical role in the bladder cancer, which may be a target beneficial for its prevention and treatment.
authors.affiliation: Department of Urology, Shanghai Changning Center Hospital, Shanghai, China. ping_baoqu@126.com
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhang
authors.forename: Ji-Hong
authors.initials: JH
authors.lastname: Wang
authors.forename: Bin
authors.initials: B
authors.lastname: Liu
authors.forename: Ping-Bao
authors.initials: PB
authors.lastname: Qu
mesh_terms: D015153:Blotting, Western; D049109:Cell Proliferation; D006801:Humans; D008099:Liver / Q000378:metabolism* / Q000473:pathology; D056921:Nuclear Receptor Coactivator 3 / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D034741:RNA, Small Interfering / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D019718:Receptors, CXCR4 / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014407:Tumor Cells, Cultured; D001749:Urinary Bladder Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*
chemical_list: C527276:CXCR4 protein, human; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D019718:Receptors, CXCR4; C538866:NCOA3 protein, human; D056921:Nuclear Receptor Coactivator 3