title: OPN -443C>T genetic polymorphism and tumor OPN expression are associated with the risk and clinical features of papillary thyroid cancer in a Chinese cohort.
abstract: OBJECTIVE
  to test the possible association between the polymorphism of Osteopontin (OPN) gene with the risk and the clinical features of papillary thyroid cancer (PTC).
  
  
  METHODS
  A total of 363 PTC patients and 413 healthy controls were enrolled. OPN expressions in tumor tissue were detected by immunohistochemistry. OPN gene polymorphisms, namely, -66T>G (rs28357094), -156G>GG (rs17524488), and -443C>T (rs11730582), were determined.
  
  
  RESULTS
  We observed that the PTC patients had significantly higher rates of -443TT genotypes than controls (P<0.001). The multivariate logistic regression analysis showed a significantly increased risk for PTC for the -443CC genotype compared with the -443TT genotype (adjusted OR= 4.312, 95%CI: 2.747 -6.987, adjusted P < 0.001). OPN protein was not expressed in normal thyroid tissues while tumor samples from PTC patients were shown to have high expressions of OPN. Also, we found that the high OPN expressions were significantly more prevalent in -443CC carriers than TT carriers (P <0.001). Both the CC carriers and OPN expression were closely associated with the cervical lymph node metastasis and angiolymphatic invasion of PTC.
  
  
  CONCLUSIONS
  This study provides evidence to support the connection between the OPN genetic polymorphism and tissue expression with risk as well as the invasiveness of PTC.
authors.affiliation: Breast Surgery Department, First Affiliated Hospital of Dalian Medical University, 11601, Dalian City, Liaoning Province, China. muguoyujason@yahoo.com.cn
authors.forename: Guoyu
authors.initials: G
authors.lastname: Mu
authors.forename: Hongjiang
authors.initials: H
authors.lastname: Wang
authors.forename: Zhengang
authors.initials: Z
authors.lastname: Cai
authors.forename: Hong
authors.initials: H
authors.lastname: Ji
mesh_terms: D000328:Adult; D000483:Alleles; D044466:Asian People / Q000235:genetics*; D002277:Carcinoma / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D002291:Carcinoma, Papillary; D002681:China; D015331:Cohort Studies; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D016015:Logistic Models; D008207:Lymphatic Metastasis; D008297:Male; D016017:Odds Ratio; D053495:Osteopontin / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D012307:Risk Factors; D000077273:Thyroid Cancer, Papillary; D013964:Thyroid Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology
chemical_list: D053495:Osteopontin