title: Production, characterization, and antioxidant activity of fucoxanthin from the marine diatom Odontella aurita.
abstract: The production, characterization, and antioxidant capacity of the carotenoid fucoxanthin from the marine diatom Odontella aurita were investigated. The results showed that low light and nitrogen-replete culture medium enhanced the biosynthesis of fucoxanthin. The maximum biomass concentration of 6.36 g L-1 and maximum fucoxanthin concentration of 18.47 mg g-1 were obtained in cultures grown in a bubble column photobioreactor (Ø 3.0 cm inner diameter), resulting in a fucoxanthin volumetric productivity of 7.96 mg L-1 day-1. A slight reduction in biomass production was observed in the scaling up of O. aurita culture in a flat plate photobioreactor, yet yielded a comparable fucoxanthin volumetric productivity. A rapid method was developed for extraction and purification of fucoxanthin. The purified fucoxanthin was identified as all-trans-fucoxanthin, which exhibited strong antioxidant properties, with the effective concentration for 50% scavenging (EC50) of 1,1-dihpenyl-2-picrylhydrazyl (DPPH) radical and 2,2'-Azino-bis(3-ethylbenzthiazoline-6-sulfonic acid (ABTS) radical being 0.14 and 0.03 mg mL-1, respectively. Our results suggested that O. aurita can be a natural source of fucoxanthin for human health and nutrition.
authors.affiliation: Institute of Hydrobiology, Jinan University, Guangzhou 510632, China. xiasongsummer212@163.com
authors.forename: Song
authors.initials: S
authors.lastname: Xia
authors.forename: Ke
authors.initials: K
authors.lastname: Wang
authors.forename: Linglin
authors.initials: L
authors.lastname: Wan
authors.forename: Aifen
authors.initials: A
authors.lastname: Li
authors.forename: Qiang
authors.initials: Q
authors.lastname: Hu
authors.forename: Chengwu
authors.initials: C
authors.lastname: Zhang
mesh_terms: D000975:Antioxidants / Q000737:chemistry* / Q000494:pharmacology*; D059001:Aquatic Organisms / Q000737:chemistry* / Q000378:metabolism; D018533:Biomass; D003470:Culture Media / Q000378:metabolism; D017377:Diatoms / Q000737:chemistry* / Q000378:metabolism; D008027:Light; D009584:Nitrogen / Q000378:metabolism; D058433:Photobioreactors / Q000382:microbiology; D024341:Xanthophylls / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D003470:Culture Media; D024341:Xanthophylls; C025164:fucoxanthin; D009584:Nitrogen