title: Polyglycolic acid-polylactic acid scaffold response to different progenitor cell in vitro cultures: a demonstrative and comparative X-ray synchrotron radiation phase-contrast microtomography study.
abstract: Spatiotemporal interactions play important roles in tissue development and function, especially in stem cell-seeded bioscaffolds. Cells interact with the surface of bioscaffold polymers and influence material-driven control of cell differentiation. In vitro cultures of different human progenitor cells, that is, endothelial colony-forming cells (ECFCs) from a healthy control and a patient with Kaposi sarcoma (an angioproliferative disease) and human CD133+ muscle-derived stem cells (MSH 133+ cells), were seeded onto polyglycolic acid-polylactic acid scaffolds. Three-dimensional (3D) images were obtained by X-ray phase-contrast microtomography (micro-CT) and processed with the Modified Bronnikov Algorithm. The method enabled high spatial resolution detection of the 3D structural organization of cells on the bioscaffold and evaluation of the way and rate at which cells modified the construct at different time points from seeding. The different cell types displayed significant differences in the proliferation rate. In conclusion, X-ray synchrotron radiation phase-contrast micro-CT analysis proved to be a useful and sensitive tool to investigate the spatiotemporal pattern of progenitor cell organization on a bioscaffold.
authors.affiliation: 1 Dipartimento di Scienze Cliniche Specialistiche e Odontostomatologiche, Università Politecnica delle Marche , Ancona, Italy .
authors.forename: Alessandra
authors.initials: A
authors.lastname: Giuliani
authors.forename: Francesca
authors.initials: F
authors.lastname: Moroncini
authors.forename: Serena
authors.initials: S
authors.lastname: Mazzoni
authors.forename: Marzia Laura Chiara
authors.initials: ML
authors.lastname: Belicchi
authors.forename: Chiara
authors.initials: C
authors.lastname: Villa
authors.forename: Silvia
authors.initials: S
authors.lastname: Erratico
authors.forename: Elena
authors.initials: E
authors.lastname: Colombo
authors.forename: Francesca
authors.initials: F
authors.lastname: Calcaterra
authors.forename: Lucia
authors.initials: L
authors.lastname: Brambilla
authors.forename: Yvan
authors.initials: Y
authors.lastname: Torrente
authors.forename: Gianni
authors.initials: G
authors.lastname: Albertini
authors.forename: Silvia
authors.initials: S
authors.lastname: Della Bella
mesh_terms: D002452:Cell Count; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay; D042783:Endothelial Cells / Q000166:cytology / Q000187:drug effects; D006801:Humans; D019344:Lactic Acid / Q000737:chemistry / Q000494:pharmacology*; D032342:Muscle Cells / Q000166:cytology / Q000187:drug effects; D011100:Polyglycolic Acid / Q000737:chemistry / Q000494:pharmacology*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D012031:Refractometry; D013234:Stem Cells / Q000166:cytology* / Q000187:drug effects*; D017356:Synchrotrons; D054457:Tissue Scaffolds / Q000737:chemistry*; D055114:X-Ray Microtomography / Q000379:methods*; D014965:X-Rays
chemical_list: D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid