title: Overexpression of a novel chrysanthemum Cys2/His2-type zinc finger protein gene DgZFP3 confers drought tolerance in tobacco.
abstract: A drought stress-responsive Cys2/His2-type zinc finger protein gene DgZFP3 was previously isolated (Liu et al., Afr J Biotechnol 11:7781-7788, 2012b) from chrysanthemum. To assess roles of DgZFP3 in plant drought stress responses, we performed gain-of-function experiment. The DgZFP3-overexpression tobacco plants showed significant drought tolerance over the wild type (WT). The transgenic lines exhibited less accumulation of H2O2 under drought stress, more accumulation of proline and greater activities of peroxidase (POD) and superoxide dismutase than the WT under both control conditions and drought stress. In addition, there was greater up-regulation of the ROS-related enzyme genes (NtSOD and NtPOD) and stress-related genes (NtLEA5 and NtDREB) in transgenic lines under normal or drought conditons. Thus DgZFP3 probably plays a positive regulatory role in drought stress response and has the potential to be utilized in transgenic breeding to improve drought stress tolerance in plants.
authors.affiliation: Department of Ornamental Horticulture, Sichuan Agricultural University, 555 Northeast Road, Wenjiang, Chengdu, 611130, Sichuan, People's Republic of China, qinglinliu@126.com.
authors.forename: Qing-Lin
authors.initials: QL
authors.lastname: Liu
authors.forename: Ke-Dong
authors.initials: KD
authors.lastname: Xu
authors.forename: Ming
authors.initials: M
authors.lastname: Zhong
authors.forename: Yuan-Zhi
authors.initials: YZ
authors.lastname: Pan
authors.forename: Bei-Bei
authors.initials: BB
authors.lastname: Jiang
authors.forename: Guang-Li
authors.initials: GL
authors.lastname: Liu
authors.forename: Yin
authors.initials: Y
authors.lastname: Jia
authors.forename: Hai-Qing
authors.initials: HQ
authors.lastname: Zhang
mesh_terms: D031202:Chrysanthemum / Q000235:genetics / Q000502:physiology*; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D003890:Desiccation; D055864:Droughts; D015870:Gene Expression; D006861:Hydrogen Peroxide / Q000378:metabolism; D009195:Peroxidase / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D013312:Stress, Physiological; D013482:Superoxide Dismutase / Q000378:metabolism; D014026:Nicotiana / Q000235:genetics / Q000502:physiology*; D016335:Zinc Fingers
chemical_list: D004268:DNA-Binding Proteins; D010940:Plant Proteins; D011994:Recombinant Proteins; D006861:Hydrogen Peroxide; D009195:Peroxidase; D013482:Superoxide Dismutase