title: Design and generation of recombinant rabies virus vectors.
abstract: Rabies viruses, negative-strand RNA viruses, infect neurons through axon terminals and spread trans-synaptically in a retrograde direction between neurons. Rabies viruses whose glycoprotein (G) gene is deleted from the genome cannot spread across synapses. Complementation of G in trans, however, enables trans-synaptic spreading of G-deleted rabies viruses to directly connected, presynaptic neurons. Recombinant rabies viruses can encode genes of interest for labeling cells, controlling gene expression and monitoring or manipulating neural activity. Cre-dependent or bridge protein-mediated transduction and single-cell electroporation via the EnvA-TVA or EnvB-TVB (envelope glycoprotein and its specific receptor for avian sarcoma leukosis virus subgroup A or B) system allow cell type-specific or single cell-specific targeting. These rabies virus-based approaches permit the linking of connectivity to cell morphology and circuit function for particular cell types or single cells. Here we describe methods for construction of rabies viral vectors, recovery of G-deleted rabies viruses from cDNA, amplification of the viruses, pseudotyping them with EnvA or EnvB and concentration and titration of the viruses. The entire protocol takes 6-8 weeks.
authors.affiliation: Systems Neurobiology Laboratories, The Salk Institute for Biological Studies, La Jolla, California, USA.
authors.forename: Fumitaka
authors.initials: F
authors.lastname: Osakada
authors.forename: Edward M
authors.initials: EM
authors.lastname: Callaway
mesh_terms: D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D017353:Gene Deletion; D005818:Genetic Engineering / Q000379:methods; D005822:Genetic Vectors; D016679:Genome, Viral; D006023:Glycoproteins / Q000235:genetics; D057809:HEK293 Cells; D006801:Humans; D009415:Nerve Net; D011820:Rabies virus / Q000235:genetics*; D014461:Ultracentrifugation; D014764:Viral Proteins / Q000235:genetics; D014776:Virus Cultivation / Q000379:methods
chemical_list: D006023:Glycoproteins; D014764:Viral Proteins