title: Metagenome-wide analysis of antibiotic resistance genes in a large cohort of human gut microbiota.
abstract: The human gut microbiota is a reservoir of antibiotic resistance genes, but little is known about their diversity and richness within the gut. Here we analyse the antibiotic resistance genes of gut microbiota from 162 individuals. We identify a total of 1,093 antibiotic resistance genes and find that Chinese individuals harbour the highest number and abundance of antibiotic resistance genes, followed by Danish and Spanish individuals. Single-nucleotide polymorphism-based analysis indicates that antibiotic resistance genes from the two European populations are more closely related while the Chinese ones are clustered separately. We also confirm high abundance of tetracycline resistance genes with this large cohort study. Our study provides a broad view of antibiotic resistance genes in the human gut microbiota.
authors.affiliation: CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Yongfei
authors.initials: Y
authors.lastname: Hu
authors.forename: Xi
authors.initials: X
authors.lastname: Yang
authors.forename: Junjie
authors.initials: J
authors.lastname: Qin
authors.forename: Na
authors.initials: N
authors.lastname: Lu
authors.forename: Gong
authors.initials: G
authors.lastname: Cheng
authors.forename: Na
authors.initials: N
authors.lastname: Wu
authors.forename: Yuanlong
authors.initials: Y
authors.lastname: Pan
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.forename: Liying
authors.initials: L
authors.lastname: Zhu
authors.forename: Xin
authors.initials: X
authors.lastname: Wang
authors.forename: Zhiqi
authors.initials: Z
authors.lastname: Meng
authors.forename: Fangqing
authors.initials: F
authors.lastname: Zhao
authors.forename: Di
authors.initials: D
authors.lastname: Liu
authors.forename: Juncai
authors.initials: J
authors.lastname: Ma
authors.forename: Nan
authors.initials: N
authors.lastname: Qin
authors.forename: Chunsheng
authors.initials: C
authors.lastname: Xiang
authors.forename: Yonghong
authors.initials: Y
authors.lastname: Xiao
authors.forename: Lanjuan
authors.initials: L
authors.lastname: Li
authors.forename: Huanming
authors.initials: H
authors.lastname: Yang
authors.forename: Jian
authors.initials: J
authors.lastname: Wang
authors.forename: Ruifu
authors.initials: R
authors.lastname: Yang
authors.forename: George F
authors.initials: GF
authors.lastname: Gao
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
authors.forename: Baoli
authors.initials: B
authors.lastname: Zhu
mesh_terms: D044466:Asian People; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics*; D041981:Gastrointestinal Tract / Q000382:microbiology*; D005798:Genes, Bacterial; D006801:Humans; D054892:Metagenome; D064307:Microbiota / Q000235:genetics*; D005810:Multigene Family; D010802:Phylogeny; D044465:White People