title: [Enzyme immunoassay of usnic acid in lichens].
abstract: An enzyme immunoassay for usnic acid in lichens was developed, the sensitivity of which was 0.1 microg/g of air-dried material (0.00001%). Polyclonal rabbit antibodies against bovine serum albumin conjugated to (+)-usnic acid under the conditions of formaldehyde condensation made it possible to determine the analyzed substance in solutions at concentrations from 1 ng/mL when it interacts with an immobilized gelatin conjugate homologous in the binding mode. Usnic acid in 2-26600 microg/g (0.0002-2.6%) amounts was found in all 236 studied samples of lichens belonging to 53 species and 8 families.
authors.forename: A A
authors.initials: AA
authors.lastname: Burkin
authors.forename: G P
authors.initials: GP
authors.lastname: Kononenko
authors.forename: T Iu
authors.initials: TIu
authors.lastname: Tolpysheva
mesh_terms: D000906:Antibodies; D000941:Antigens / Q000276:immunology; D001572:Benzofurans / Q000276:immunology / Q000302:isolation & purification*; D007124:Immunoenzyme Techniques; D008011:Lichens / Q000145:classification / Q000276:immunology / Q000302:isolation & purification*
chemical_list: D000906:Antibodies; D000941:Antigens; D001572:Benzofurans; C073339:usnic acid