title: SPE-HPLC-MS/MS method for the trace analysis of tobacco-specific N-nitrosamines and 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol in rabbit plasma using tetraazacalix[2]arene[2]triazine-modified silica as a sorbent.
abstract: Tobacco-specific N-nitrosamines (TSNAs), including N'-nitrosonornicotine, 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone, N'-nitrosoanatabine, and N'-nitrosoanabasine, have been implicated as a source of carcinogenicity in tobacco and cigarette smoke. We present a rapid and effective method comprising SPE based on tetraazacalix[2]arene[2]triazine-modified silica as sorbent and analysis with HPLC-MS/MS for the determination of TSNAs and 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol (NNAL), a metabolite of 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone, in rabbit plasma. The linear dynamic ranges were 10-2000 pg/mL for NNAL and 4-2000 pg/mL for the four TSNAs with good correlation coefficients (>0.9965). The LODs were in the range of 0.9-3.7 pg/mL, and the LOQs were between 2.9 and 12.3 pg/mL. The accuracies of the method were also evaluated and found to be in the range of 90.1-113.3%. This method is promising to be applied to the preconcentration and determination of TSNAs and NNAL in smoke and human body fluids.
authors.affiliation: Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou, PR China.
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.forename: Chunqiang
authors.initials: C
authors.lastname: Yang
authors.forename: Qidong
authors.initials: Q
authors.lastname: Zhang
authors.forename: Sheng
authors.initials: S
authors.lastname: Wang
authors.forename: Hailei
authors.initials: H
authors.lastname: Lang
authors.forename: Shusheng
authors.initials: S
authors.lastname: Zhang
authors.forename: Wenjie
authors.initials: W
authors.lastname: Zhao
authors.forename: Jianxun
authors.initials: J
authors.lastname: Zhang
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D009602:Nitrosamines / Q000097:blood* / Q000302:isolation & purification*; D011725:Pyridines / Q000097:blood* / Q000302:isolation & purification*; D011817:Rabbits; D012822:Silicon Dioxide / Q000737:chemistry; D052616:Solid Phase Extraction / Q000295:instrumentation / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D009602:Nitrosamines; D011725:Pyridines; D012822:Silicon Dioxide; C099565:4-(methylnitrosamino)-1-(3-pyridyl)-1-butan-1-ol
keywords: Liquid chromatography-tandem mass spectrometry; N-nitrosamines; Plasma matrices; Solid phase extraction; Tobacco