title: The effect of anti-inflammatory and antimicrobial herbal remedy PADMA 28 on immunological angiogenesis and granulocytes activity in mice.
abstract: PADMA 28 is a herbal multicompound remedy that originates from traditional Tibetan medicine and possesses anti-inflammatory, antioxidant, antimicrobial, angioprotecting, and wound healing properties. The aim of the present study was to evaluate the influence of this remedy on immunological angiogenesis and granulocytes metabolic activity in Balb/c mice. Mice were fed daily, for seven days, with 5.8 mg of PADMA (calculated from recommended human daily dose) or 0.085 mg (dose in the range of active doses of other herbal extracts studied by us previously).
  
  
  RESULTS
  Highly significant increase of newly formed blood vessels number in ex vivo cutaneous lymphocyte-induced angiogenesis test (LIA) after grafting of Balb/c splenocytes from both dosage groups to F1 hybrids (Balb/c × C3H); increase of blood lymphocytes and granulocytes number only in mice fed with lower dose of remedy; and significant suppression of metabolic activity (chemiluminescence test) of blood granulocytes in mice fed with higher dose of PADMA.
  
  
  CONCLUSIONS
  PADMA 28 behaves as a good stimulator of physiological angiogenesis, but for this purpose it should be used in substantially lower doses than recommended by producers for avoiding the deterioration of granulocyte function.
authors.affiliation: Department of Histology and Embryology, Center for Biostructure Research, Warsaw Medical University, Chałubinskiego 5, 02-004 Warsaw, Poland.
authors.forename: Dorota M
authors.initials: DM
authors.lastname: Radomska-Leśniewska
authors.forename: Piotr
authors.initials: P
authors.lastname: Skopiński
authors.forename: Marcin
authors.initials: M
authors.lastname: Niemcewicz
authors.forename: Robert
authors.initials: R
authors.lastname: Zdanowski
authors.forename: Sławomir
authors.initials: S
authors.lastname: Lewicki
authors.forename: Janusz
authors.initials: J
authors.lastname: Kocik
authors.forename: Ewa
authors.initials: E
authors.lastname: Skopińska-Różewska
authors.forename: Wanda
authors.initials: W
authors.lastname: Stankiewicz
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000494:pharmacology*; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000378:metabolism; D005260:Female; D006098:Granulocytes / Q000187:drug effects*; D007962:Leukocytes / Q000187:drug effects; D049449:Luminescence; D008214:Lymphocytes / Q000187:drug effects; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D009389:Neovascularization, Pathologic; D010936:Plant Extracts / Q000494:pharmacology*
chemical_list: D000890:Anti-Infective Agents; D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D010936:Plant Extracts; C066069:Padma 28