title: Effect of Glu-B3 allelic variation on sodium dodecyl sulfate sedimentation volume in common wheat (Triticum aestivum L.).
abstract: Sodium dodecyl sulfate (SDS) sedimentation volume has long been used to characterize wheat flours and meals with the aim of predicting processing and end-product qualities. In order to survey the influence of low-molecular-weight glutenin subunits (LMW-GSs) at Glu-B3 locus on wheat SDS sedimentation volume, a total of 283 wheat (Triticum aestivum L.) varieties including landraces and improved and introduced cultivars were analyzed using 10 allele-specific PCR markers at the Glu-B3 locus. The highest allele frequency observed in the tested varieties was Glu-B3i with 21.9% in all varieties, 21.1% in landraces, 25.5% in improved cultivars, and 12% in introduced cultivars. Glu-B3 locus represented 8.6% of the variance in wheat SDS sedimentation volume, and Glu-B3b, Glu-B3g, and Glu-B3h significantly heightened the SDS sedimentation volume, but Glu-B3a, Glu-B3c, and Glu-B3j significantly lowered the SDS sedimentation volume. For the bread-making quality, the most desirable alleles Glu-B3b and Glu-B3g become more and more popular and the least desirable alleles Glu-B3a and Glu-B3c got less and less in modern improved cultivars, suggesting that wheat grain quality in China has been significantly improved through breeding effort.
authors.affiliation: School of Agronomy, Anhui Agricultural University, Hefei 230036, China.
authors.forename: Hongqi
authors.initials: H
authors.lastname: Si
authors.forename: Manli
authors.initials: M
authors.lastname: Zhao
authors.forename: Fuxia
authors.initials: F
authors.lastname: He
authors.forename: Chuanxi
authors.initials: C
authors.lastname: Ma
mesh_terms: D000483:Alleles; D031521:Fractionation, Field Flow / Q000379:methods*; D014644:Genetic Variation / Q000235:genetics*; D005983:Glutens / Q000737:chemistry* / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry*; D014908:Triticum / Q000737:chemistry* / Q000235:genetics*
chemical_list: D012967:Sodium Dodecyl Sulfate; D005983:Glutens; C037302:glutenin