title: The combined influence of substrate elasticity and surface-grafted molecules on the ex vivo expansion of hematopoietic stem and progenitor cells.
abstract: Umbilical cord blood (UCB) is an attractive source of hematopoietic stem and progenitor cells (HSPCs) for transplantation. However, the low number of HSPCs from a single UCB donor limits the direct transplantation of UCB to patients. Because little is known about the effects of the physical microenvironment on HSPC expansion, we investigated the ex vivo expansion of HSPCs cultured on biomaterials with different elasticities and grafted with different nanosegments. Polyvinylalcohol-co-itaconic acid (PVA-IA)-coated dishes with different stiffnesses ranging from a 3.7 kPa to 30.4 kPa storage modulus were used. Fibronectin or an oligopeptide (CS1, EILDVPST) was grafted onto the PVA-IA substrates. High ex vivo fold expansion of HSPCs was observed in the PVA-IA dishes grafted with fibronectin or CS1, which displayed an intermediate stiffness ranging from 12.2 kPa to 30.4 kPa. The fold expansion was more than 1.4 times higher than that cultured in tissue culture polystyrene dishes (TCPS, 12 GPa). Furthermore, HSPCs cultured in fibronectin or CS1-grafted PVA-IA-coated dishes with a stiffness of 12.2-30.4 kPa generated more pluripotent colony-forming units (CFU-GM and CFU-GEMM) than those in TCPS dishes. This result indicates that both the physical and biological properties of biomaterials affect the ex vivo expansion of HSPCs.
authors.affiliation: Department of Chemical and Materials Engineering, National Central University, Jhongli, Taoyuan 32001, Taiwan, ROC.
authors.forename: Suresh S
authors.initials: SS
authors.lastname: Kumar
authors.forename: Jui-Hsiang
authors.initials: JH
authors.lastname: Hsiao
authors.forename: Qing-Dong
authors.initials: QD
authors.lastname: Ling
authors.forename: Ida
authors.initials: I
authors.lastname: Dulinska-Molak
authors.forename: Guoping
authors.initials: G
authors.lastname: Chen
authors.forename: Yung
authors.initials: Y
authors.lastname: Chang
authors.forename: Yu
authors.initials: Y
authors.lastname: Chang
authors.forename: Yung Hung
authors.initials: YH
authors.lastname: Chen
authors.forename: Da-Chung
authors.initials: DC
authors.lastname: Chen
authors.forename: Shih-Tien
authors.initials: ST
authors.lastname: Hsu
authors.forename: Akon
authors.initials: A
authors.lastname: Higuchi
mesh_terms: D049109:Cell Proliferation; D005353:Fibronectins / Q000378:metabolism; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans; D009842:Oligopeptides / Q000378:metabolism; D013234:Stem Cells / Q000166:cytology*
chemical_list: D005353:Fibronectins; D009842:Oligopeptides
keywords: Blood; Cell proliferation; Elasticity; Fibronectin; Stem cell; Surface grafting