title: Enzymatic production of trans fatty acid free fat from partially hydrogenated soybean oil (PHSO)--theory, strategy and practicability.
abstract: Development of an advanced process/production technology for healthful fats constitutes a major interest of plant oil refinery industry. In this work, a strategy to produce trans fatty acid (TFA) free (or low TFA) products from partially hydrogenated soybean oil by lipase-catalysed selective hydrolysis was proposed, where a physically founded mathematic model to delineate the multi-responses of the reaction as a function of selectivity factor was defined for the first time. The practicability of this strategy was assessed with commercial trans-selective Candida antarctica lipase A (CAL-A) as a model biocatalyst based on a parameter study and fitting to the model. CAL-A was found to have a selectivity factor 4.26 and to maximally remove 73.3% of total TFAs at 46.5% hydrolysis degree.
authors.affiliation: Department of Engineering, Aarhus University, DK-8000 Aarhus C, Denmark.
authors.forename: Ram Chandra Reddy
authors.initials: RC
authors.lastname: Jala
authors.forename: Xuebing
authors.initials: X
authors.lastname: Xu
authors.forename: Zheng
authors.initials: Z
authors.lastname: Guo
mesh_terms: D055162:Biocatalysis; D002175:Candida / Q000201:enzymology*; D005524:Food Technology / Q000379:methods*; D005656:Fungal Proteins / Q000737:chemistry*; D006865:Hydrogenation; D008049:Lipase / Q000737:chemistry*; D008962:Models, Theoretical; D015394:Molecular Structure; D013024:Soybean Oil / Q000737:chemistry*; D044242:Trans Fatty Acids / Q000737:chemistry*
chemical_list: D005656:Fungal Proteins; D044242:Trans Fatty Acids; D013024:Soybean Oil; D008049:Lipase
keywords: Candida antarctica lipase A (CAL-A); Partially hydrogenated soybean oil (PHSO); Selective hydrolysis; Trans fatty acids (TFAs); Trans selectivity factor