title: Serum methylation levels of TAC1. SEPT9 and EYA4 as diagnostic markers for early colorectal cancers: a pilot study.
abstract: OBJECTIVE
  To identify methylated genes in serum with diagnostic potentials for early colorectal cancer (CRC).
  
  
  METHODS
  Serum methylation levels of up to 12 genes were measured in two sets of serum samples with the second set from 26 stage I CRC patients and 26 age/gender-matched controls.
  
  
  RESULTS
  Serum methylation levels of TAC1, SEPT9, and EYA4 were significant discriminants between stage I CRC and healthy controls. Combination of TAC1 and SEPT9 rendered 73.1% sensitivity with 92.3% specificity.
  
  
  CONCLUSIONS
  Serum methylation levels of TAC1. SEPT9 and EYA4 may be useful biomarkers for early detection of CRC though a validation study is necessary.
authors.affiliation: Department of Colorectal Surgery, Singapore General Hospital, Singapore. liu.yan.qun@sgh.com.sg
authors.forename: Yanqun
authors.initials: Y
authors.lastname: Liu
authors.forename: Chee Kian
authors.initials: CK
authors.lastname: Tham
authors.forename: Simon Y K
authors.initials: SY
authors.lastname: Ong
authors.forename: Kok Sun
authors.initials: KS
authors.lastname: Ho
authors.forename: Jit Fong
authors.initials: JF
authors.lastname: Lim
authors.forename: Min Hoe
authors.initials: MH
authors.lastname: Chew
authors.forename: Che Kang
authors.initials: CK
authors.lastname: Lim
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhao
authors.forename: Choong Leong
authors.initials: CL
authors.lastname: Tang
authors.forename: Kong Weng
authors.initials: KW
authors.lastname: Eu
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D019540:Area Under Curve; D014408:Biomarkers, Tumor / Q000097:blood* / Q000235:genetics; D016022:Case-Control Studies; D015179:Colorectal Neoplasms / Q000097:blood* / Q000175:diagnosis / Q000235:genetics; D019175:DNA Methylation; D055088:Early Detection of Cancer; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D010865:Pilot Projects; D011446:Prospective Studies; D012372:ROC Curve; D058112:Septins / Q000097:blood* / Q000235:genetics; D015534:Trans-Activators / Q000097:blood* / Q000235:genetics; D053303:Transmembrane Activator and CAML Interactor Protein / Q000097:blood* / Q000235:genetics
chemical_list: D014408:Biomarkers, Tumor; C117736:EYA4 protein, human; C108467:TNFRSF13B protein, human; D015534:Trans-Activators; D053303:Transmembrane Activator and CAML Interactor Protein; C474315:SEPTIN9 protein, human; D058112:Septins