title: [Interactions of 1A2 insulator with promoter of hsp 70 gene in Drosophila melanogaster].
abstract: Insulators are regulatory DNA elements that participate in the modulation of the interactions between enhancers and promoters. Depending on the situation, insulators can either stabilize or destroy the contacts between enhancers and promoters. A possible explanation for the activity of insulators is their ability to directly interact with gene promoters. In the present study, it was demonstrated that, in model systems, a 1A2 insulator could interact with the core sequence of an hsp70 promoter. In this case, the insulator protein CP190 is found on the hsp70 promoter, which depends on the presence of an insulator in the transgene. The data obtained are consistent with the model, which implies that direct contacts between insulators and promoters make a considerable contribution to the modulation of the interactions between insulators and promoters.
authors.forename: D A
authors.initials: DA
authors.lastname: Chetverina
authors.forename: P V
authors.initials: PV
authors.lastname: Elizar'ev
authors.forename: P G
authors.initials: PG
authors.lastname: Georgiev
authors.forename: M M
authors.initials: MM
authors.lastname: Erokhin
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D030801:Animals, Genetically Modified; D029721:Drosophila Proteins / Q000235:genetics*; D004331:Drosophila melanogaster / Q000235:genetics*; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics*; D038101:Insulator Elements; D008869:Microtubule-Associated Proteins / Q000235:genetics; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000235:genetics; D011401:Promoter Regions, Genetic; D019076:Transgenes
chemical_list: C107964:CP190 protein, Drosophila; D029721:Drosophila Proteins; D018840:HSP70 Heat-Shock Proteins; D008869:Microtubule-Associated Proteins; D009687:Nuclear Proteins; C050680:y protein, Drosophila