title: Evaluation of sustained drug delivery for bone morphogenetic protein on the functionalized nanotubular surface.
abstract: An effective loading method of the recombinant human Bone Morphogenetic Protein-2 (rhBMP-2) on the implant surface is essential for promoting bone formation. However, the studies comparing the effectiveness of loading methods for rhBMP-2 are insufficient. The objective of this study was to investigate the sustained rhBMP-2 delivery on the functionalized nanotubular surface. rhBMP-2 loading method on functionalized nanotubular surface was divided into 3 groups; coating and dry method, vacuum method, lyophilization method. A total of 10 ug rhBMP-2 was loaded into functionalized nanotubular titanium implant surfaces (3.75 mm in diameter and 7 mm in length). The releasing time of rhBMP-2 was evaluated at 1, 5, 7 days. The data were statistically analyzed with one-way ANOVA and a Post Hoc Bonferroni test. A sustained release pattern was observed in each groups and the amount of rhBMP-2 loaded was the highest in the lyophilization group. The results suggest that lyophilization method is effective method to load rhBMP-2 on the functionalized nanotubular surface and the functionalized nanotubular surface could be a sustainable useful carrier of rhBMP-2.
authors.affiliation: School of Dentistry, Dental Science Research Instituition, Chonnam National University, Gwangju 500-757, Republic of Korea.
authors.forename: Juri
authors.initials: J
authors.lastname: Ma
authors.forename: Kwangmin
authors.initials: K
authors.lastname: Lee
authors.forename: Jaesam
authors.initials: J
authors.lastname: Ban
authors.forename: Hyeju
authors.initials: H
authors.lastname: Son
authors.forename: Jeongtae
authors.initials: J
authors.lastname: Koh
authors.forename: Gabwoon
authors.initials: G
authors.lastname: Hwang
authors.forename: Hyunseung
authors.initials: H
authors.lastname: Kim
authors.forename: Kyungku
authors.initials: K
authors.lastname: Lee
authors.forename: Mongsook
authors.initials: M
authors.lastname: Vang
authors.forename: Hongso
authors.initials: H
authors.lastname: Yang
authors.forename: Hyunpil
authors.initials: H
authors.lastname: Lim
authors.forename: Gyejeong
authors.initials: G
authors.lastname: Oh
authors.forename: Sangwon
authors.initials: S
authors.lastname: Park
authors.forename: Kwidug
authors.initials: K
authors.lastname: Yun
mesh_terms: D055396:Bone Morphogenetic Protein 2 / Q000008:administration & dosage*; D016503:Drug Delivery Systems; D004566:Electrodes; D006801:Humans; D007700:Kinetics; D043942:Nanotubes; D010084:Oxidation-Reduction; D011994:Recombinant Proteins / Q000008:administration & dosage
chemical_list: D055396:Bone Morphogenetic Protein 2; D011994:Recombinant Proteins