title: Transglutaminase-mediated macromolecular assembly: production of conjugates for food and pharmaceutical applications.
abstract: Various strategies have been explored in the last 20 years to modify the functional properties of proteins and, among these, protein/polymer conjugation resulted one of the most successful approaches. Thus, the surface modification of polypeptides of potential industrial interest by covalent attachment of different macromolecules is nowadays regarded as an extremely valuable technique to manipulate protein activities. Protein derivatives with a number of either natural or synthetic polymers, like different polysaccharides or polyethylene glycol, have been obtained by both chemical and enzymatic treatments, and in this context, the crosslinking enzyme transglutaminase is attracting an increasing attention as a simple and safe means for protein processing in vitro. In this short review, we summarized the most significant experimental findings demonstrating that a microbial form of the enzyme is an effective tool to obtain several biopolymer-based conjugates potentially useful for both food and pharmaceutical applications.
authors.affiliation: Department of Chemical Sciences, University of Naples "Federico II", Complesso Universitario di Monte S. Angelo, Via Cinthia, 80126, Naples, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Mariniello
authors.forename: R
authors.initials: R
authors.lastname: Porta
authors.forename: A
authors.initials: A
authors.lastname: Sorrentino
authors.forename: C V L
authors.initials: CV
authors.lastname: Giosafatto
authors.forename: G
authors.initials: G
authors.lastname: Rossi Marquez
authors.forename: M
authors.initials: M
authors.lastname: Esposito
authors.forename: P
authors.initials: P
authors.lastname: Di Pierro
mesh_terms: D005502:Food; D046911:Macromolecular Substances / Q000378:metabolism*; D004364:Pharmaceutical Preparations; D011503:Transglutaminases / Q000378:metabolism*
chemical_list: D046911:Macromolecular Substances; D004364:Pharmaceutical Preparations; D011503:Transglutaminases