title: [Construction of cell surface display system in lactic acid bacteria by using ice nucleation protein].
abstract: OBJECTIVE
  The gene of ice nucleation protein (INP), an outer membrane of Pseudomonas syringae was tested to display foreign proteins on the surface of Lactococcus lactis.
  
  
  METHODS
  Plasmids pHZ101 and pHZ102 were constructed using gfp (Green Fluorescence Protein) as the reporter gene and N-terminal and NC-terminal of inp as the anchoring units. Plasmids pHZ101 and pHZ102 were subsequently transformed into Escherichia coli JM109 and Lactococcus lactis MG1363.
  
  
  RESULTS
  Fluorescence microscope shows that green fluorescence was observed in both recombinant E. coli and L. lactis strains. Western blot indicated that GFP was expressed in both recombinant E. coli and L. lactis strains. INPN-GFP was mostly trapped in cytoplastic fraction while INPNC-GFP was mainly targeted on the cell membrane of L. lactis.
  
  
  CONCLUSIONS
  The results suggest a new way to construct cell surface display system of lactic acid bacteria by using ice nucleation protein.
authors.affiliation: State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China. zhangqx@jiangnan.edu.cn
authors.forename: Qiuxiang
authors.initials: Q
authors.lastname: Zhang
authors.forename: Huili
authors.initials: H
authors.lastname: Hou
authors.forename: Ying
authors.initials: Y
authors.lastname: Lu
authors.forename: Wei
authors.initials: W
authors.lastname: Chen
authors.forename: Jin
authors.initials: J
authors.lastname: Zhong
mesh_terms: D001425:Bacterial Outer Membrane Proteins / Q000235:genetics* / Q000378:metabolism*; D002462:Cell Membrane / Q000235:genetics* / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D019344:Lactic Acid / Q000378:metabolism*; D013294:Lactococcus lactis / Q000235:genetics* / Q000378:metabolism*; D010957:Plasmids / Q000235:genetics; D044224:Pseudomonas syringae / Q000235:genetics / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D001425:Bacterial Outer Membrane Proteins; D011993:Recombinant Fusion Proteins; C063113:ice nucleation protein; D049452:Green Fluorescent Proteins; D019344:Lactic Acid