title: A new minimally invasive mesotherapy technique for facial rejuvenation.
abstract: BACKGROUND
  This study describes a pivotal clinical trial of a new minimally invasive mesotherapy technique for facial rejuvenation.
  
  
  METHODS
  The authors utilized two formulations: formulation A with hyaluronic acid, vitamins, amino acids, minerals, coenzymes, and antioxidant substances; formulation B with hyaluronic acid and idebenone. Fifty participants were enrolled in the study and divided in two groups. Group 1 (50-65 years) treated with formulation A. Group 2 (35-50 years) treated with formulation B. The groups underwent four sessions of mesotherapy involving multiple injections. Treatment was conducted at 15 day intervals. All participants had pre- and posttreatment photographs. Punch biopsies were taken from randomly selected participants, baseline and after 6 weeks, and stained for interleukin (IL)-6, IL-1β, matrix metalloproteinase (MMP)-1, and collagen 1. Clinical evaluation was based on the Global Aesthetic Scale (GAIS) and on the Wrinkle Severity Rating Scale (WSRS).
  
  
  RESULTS
  The results produced were statistically analyzed and resulted in a significant and long-lasting effect on facial rejuvenation. Evaluation of photographs at 0, 1, and 2 months revealed significant clinical improvement: brightness, texture, and firmness of the skin. The analysis of the GAIS and WSRS scores in the two groups demonstrated statistically significant results after 2 months. The biopsies taken from randomly selected participants at baseline and after 3 months showed a decrease in IL-1β, IL-6, and MMP1, and an increase in collagen 1.
  
  
  CONCLUSIONS
  The new minimally invasive mesotherapy technique described can improve the clinical appearance of the skin in different age groups.
authors.affiliation: Promoitalia Group S.p.A, Pozzuoli, Naples Italy.
authors.forename: Antonella
authors.initials: A
authors.lastname: Savoia
authors.forename: Simone
authors.initials: S
authors.lastname: Landi
authors.forename: Alfonso
authors.initials: A
authors.lastname: Baldi
keywords: Biorevitalization; Cosmetic dermatology; Facial rejuvenation; Fillers; Mesotherapy; Soft-tissue augmentation