title: [Isolation, idetification and anti-HIV-1 integrase activity of culturable endophytic fungi from Tibetan medicinal plant Phlomis younghusbandii Mukerjee].
abstract: A total of 52 endophytic fungi were isolated from roots and stems of Tibetan medicinal plant Phlomis younghusbandii Mukerjee. These fungal isolates were molecularly identified based on ITS sequnces and 28S sequences distributed to 12 genera, including Phoma, Chaetosphaeronema, Fusarium and Leptosphaeria, etc. Among them, the dominant genus was Phoma. Extracts of all strains were evaluated for anti-HIV-1 integrase activity by using soluable integrase expressed in E. coli BL21 (DE3). The results showed that seven samples from five fungal endophytes PHY-24, PHY-38, PHY-40, PHY-51, PHY-53, which belonged to genus Chaetosphaeronema, inhibited strand transfer reaction catalyzed by HIV-1 integrase with IC50 values, of 6.60, 5.20, 2.86, 7.86, 4.47, 4.56 and 3.23 microg x mL(-1) respectively. In conclusion, the endophytic fungi of Phlomis younghusbandii Mukerjee are valuable for further screening anti-HIV-1 integrase agents.
authors.affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China.
authors.forename: Da-Wei
authors.initials: DW
authors.lastname: Zhang
authors.forename: Ming-Ming
authors.initials: MM
authors.lastname: Zhao
authors.forename: Juan
authors.initials: J
authors.lastname: Chen
authors.forename: Chao
authors.initials: C
authors.lastname: Li
authors.forename: Shun-Xing
authors.initials: SX
authors.lastname: Guo
mesh_terms: D001203:Ascomycota / Q000201:enzymology* / Q000302:isolation & purification; D002597:Chaetomium / Q000201:enzymology / Q000302:isolation & purification; D060026:Endophytes / Q000201:enzymology* / Q000302:isolation & purification; D004926:Escherichia coli / Q000201:enzymology; D019427:HIV Integrase / Q000235:genetics / Q000378:metabolism*; D019428:HIV Integrase Inhibitors / Q000494:pharmacology*; D031345:Phlomis / Q000382:microbiology*; D010802:Phylogeny; D018517:Plant Roots / Q000382:microbiology; D018547:Plant Stems / Q000382:microbiology; D010946:Plants, Medicinal / Q000382:microbiology; D010957:Plasmids; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D019428:HIV Integrase Inhibitors; D011994:Recombinant Proteins; D019427:HIV Integrase; C514801:p31 integrase protein, Human immunodeficiency virus 1