title: High-speed panoramic light-sheet microscopy reveals global endodermal cell dynamics.
abstract: The ever-increasing speed and resolution of modern microscopes make the storage and post-processing of images challenging and prevent thorough statistical analyses in developmental biology. Here, instead of deploying massive storage and computing power, we exploit the spherical geometry of zebrafish embryos by computing a radial maximum intensity projection in real time with a 240-fold reduction in data rate. In our four-lens selective plane illumination microscope (SPIM) setup the development of multiple embryos is recorded in parallel and a map of all labelled cells is obtained for each embryo in <10 s. In these panoramic projections, cell segmentation and flow analysis reveal characteristic migration patterns and global tissue remodelling in the early endoderm. Merging data from many samples uncover stereotypic patterns that are fundamental to endoderm development in every embryo. We demonstrate that processing and compressing raw image data in real time is not only efficient but indispensable for image-based systems biology.
authors.affiliation: Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstr. 108, 01307 Dresden, Germany.
authors.forename: Benjamin
authors.initials: B
authors.lastname: Schmid
authors.forename: Gopi
authors.initials: G
authors.lastname: Shah
authors.forename: Nico
authors.initials: N
authors.lastname: Scherf
authors.forename: Michael
authors.initials: M
authors.lastname: Weber
authors.forename: Konstantin
authors.initials: K
authors.lastname: Thierbach
authors.forename: Citlali Pérez
authors.initials: CP
authors.lastname: Campos
authors.forename: Ingo
authors.initials: I
authors.lastname: Roeder
authors.forename: Pia
authors.initials: P
authors.lastname: Aanstad
authors.forename: Jan
authors.initials: J
authors.lastname: Huisken
mesh_terms: D000818:Animals; D019521:Body Patterning; D002454:Cell Differentiation; D002465:Cell Movement; D004625:Embryo, Nonmammalian; D004707:Endoderm / Q000166:cytology* / Q000196:embryology; D007091:Image Processing, Computer-Assisted / Q000295:instrumentation* / Q000379:methods; D008027:Light; D008856:Microscopy, Fluorescence / Q000295:instrumentation* / Q000379:methods; D015027:Zebrafish / Q000033:anatomy & histology* / Q000196:embryology