title: Differential ABCB and ABCC gene expression and efflux activities in gills and hemocytes of Mytilus galloprovincialis and their involvement in cadmium response.
abstract: The aim of the study was to characterize ABC transport proteins gene expression and efflux activities in gills and hemocytes of the Mediterranean mussel and to evaluate their response to Cd. At basal level a higher expression of abcb-like gene was observed in gills than in hemocytes while abcc-like gene showed similar levels. Both P-gp and MRPs inhibitors (cyclosporine and MK571) blocked efflux activities in gills; hemocytes were sensitive only to MK571. After 120 min in vitro pre-exposure to CdCl2, the efflux activity increased significantly in gills and hemocytes. In vivo exposure to CdCl2 (0.4 μM) increased abcb-like gene expression in gills without affecting efflux activity. In hemocytes abcc-like gene resulted up-regulated and Ca-AM efflux resulted enhanced. An increased uptake of Cd in gills biopsies was observed in the presence of both P-gp and MRPs inhibitors. Our results indicate that ABC transporters seem involved in the first protective response to Cd and this response is tissue-specific.
authors.affiliation: Department of Physical, Earth and Environmental Sciences, University of Siena, Via Mattioli 4, 53100 Siena, Italy. Electronic address: dellatorre2@unisi.it.
authors.forename: Camilla
authors.initials: C
authors.lastname: Della Torre
authors.affiliation: Department of Physical, Earth and Environmental Sciences, University of Siena, Via Mattioli 4, 53100 Siena, Italy.
authors.forename: Elena
authors.initials: E
authors.lastname: Bocci
authors.affiliation: Department of Physical, Earth and Environmental Sciences, University of Siena, Via Mattioli 4, 53100 Siena, Italy.
authors.forename: Silvano Ettore
authors.initials: SE
authors.lastname: Focardi
authors.affiliation: Department of Physical, Earth and Environmental Sciences, University of Siena, Via Mattioli 4, 53100 Siena, Italy.
authors.forename: Ilaria
authors.initials: I
authors.lastname: Corsi
mesh_terms: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D002104:Cadmium / Q000493:pharmacokinetics / Q000633:toxicity*; D016572:Cyclosporine / Q000494:pharmacology; D005452:Fluoresceins / Q000494:pharmacology; D005456:Fluorescent Dyes / Q000494:pharmacology; D005786:Gene Expression Regulation / Q000187:drug effects; D005880:Gills / Q000187:drug effects / Q000378:metabolism; D006434:Hemocytes / Q000187:drug effects / Q000378:metabolism; D027425:ATP-Binding Cassette, Sub-Family C Proteins / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D049878:Mytilus / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D011422:Propionates / Q000494:pharmacology; D011804:Quinolines / Q000494:pharmacology; D014874:Water Pollutants, Chemical / Q000493:pharmacokinetics / Q000633:toxicity*
chemical_list: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D002104:Cadmium; D016572:Cyclosporine; D005452:Fluoresceins; D005456:Fluorescent Dyes; D027425:ATP-Binding Cassette, Sub-Family C Proteins; D011422:Propionates; D011804:Quinolines; D014874:Water Pollutants, Chemical; C085925:calcein AM; C059141:verlukast
keywords: ABC transport proteins; Cadmium; Efflux activity; Mediterranean mussel; mRNA expression