title: Nonstructural 5A protein of hepatitis C virus interacts with pyruvate carboxylase and modulates viral propagation.
abstract: Hepatitis C virus (HCV) is highly dependent on cellular factors for its own propagation. By employing tandem affinity purification method, we identified pyruvate carboxylase (PC) as a cellular partner for NS5A protein. NS5A interacted with PC through the N-terminal region of NS5A and the biotin carboxylase domain of PC. PC expression was decreased in cells expressing NS5A and HCV-infected cells. Promoter activity of PC was also decreased by NS5A protein. However, FAS expression was increased in cells expressing NS5A and cell culture grown HCV (HCVcc)-infected cells. Silencing of PC promoted fatty acid synthase (FAS) expression level. These data suggest HCV may modulate PC via NS5A protein for its own propagation.
authors.affiliation: National Research Laboratory of Hepatitis C Virus, Ilsong Institute of Life Science, Hallym University, Anyang, Korea.
authors.forename: Seung-Ae
authors.initials: SA
authors.lastname: Yim
authors.forename: Yun-Sook
authors.initials: YS
authors.lastname: Lim
authors.forename: Jong-Wook
authors.initials: JW
authors.lastname: Kim
authors.forename: Soon B
authors.initials: SB
authors.lastname: Hwang
mesh_terms: D000595:Amino Acid Sequence; D002460:Cell Line; D054890:Fatty Acid Synthase, Type I / Q000235:genetics / Q000378:metabolism*; D005786:Gene Expression Regulation; D016174:Hepacivirus / Q000235:genetics / Q000378:metabolism*; D022781:Hepatocytes / Q000201:enzymology* / Q000821:virology; D054884:Host-Pathogen Interactions; D006801:Humans; D050356:Lipid Metabolism; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D011766:Pyruvate Carboxylase / Q000235:genetics / Q000378:metabolism*; D017361:Viral Nonstructural Proteins / Q000235:genetics / Q000378:metabolism*; D014779:Virus Replication; D012324:RNA-Dependent RNA Polymerase
chemical_list: D054890:Fatty Acid Synthase, Type I; D011766:Pyruvate Carboxylase; D017361:Viral Nonstructural Proteins; C518729:FASN protein, human; C087036:NS-5 protein, hepatitis C virus; D012324:RNA-Dependent RNA Polymerase