title: Transcription factor binding profiles reveal cyclic expression of human protein-coding genes and non-coding RNAs.
abstract: Cell cycle is a complex and highly supervised process that must proceed with regulatory precision to achieve successful cellular division. Despite the wide application, microarray time course experiments have several limitations in identifying cell cycle genes. We thus propose a computational model to predict human cell cycle genes based on transcription factor (TF) binding and regulatory motif information in their promoters. We utilize ENCODE ChIP-seq data and motif information as predictors to discriminate cell cycle against non-cell cycle genes. Our results show that both the trans- TF features and the cis- motif features are predictive of cell cycle genes, and a combination of the two types of features can further improve prediction accuracy. We apply our model to a complete list of GENCODE promoters to predict novel cell cycle driving promoters for both protein-coding genes and non-coding RNAs such as lincRNAs. We find that a similar percentage of lincRNAs are cell cycle regulated as protein-coding genes, suggesting the importance of non-coding RNAs in cell cycle division. The model we propose here provides not only a practical tool for identifying novel cell cycle genes with high accuracy, but also new insights on cell cycle regulation by TFs and cis-regulatory elements.
authors.affiliation: Department of Genetics, Geisel School of Medicine at Dartmouth, Hanover, New Hampshire, USA. chao.cheng@dartmouth.edu
authors.forename: Chao
authors.initials: C
authors.lastname: Cheng
authors.forename: Matthew
authors.initials: M
authors.lastname: Ung
authors.forename: Gavin D
authors.initials: GD
authors.lastname: Grant
authors.forename: Michael L
authors.initials: ML
authors.lastname: Whitfield
mesh_terms: D018816:Genes, cdc; D006801:Humans; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D022661:RNA, Untranslated / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: D022661:RNA, Untranslated; D014157:Transcription Factors