title: Multivariate miRNA signatures as biomarkers for non-ischaemic systolic heart failure.
abstract: OBJECTIVE
  Non-ischaemic heart failure is one of the today's most prevalent cardiovascular disorders. Since modern pharmacotherapy has proved to be very effective in delaying disease progression and preventing death, imaging modalities and molecular biomarkers play an important role in early identification and clinical management as well as risk assessment of patients. The present study evaluated for the first time whole peripheral blood miRNAs as novel biomarker candidates for non-ischaemic heart failure with reduced ejection fraction (HF-REF).
  
  
  RESULTS
  We assessed genome-wide miRNA expression profiles in 53 HF-REF patients and 39 controls. We could identify and validate several miRNAs that show altered expression levels in non-ischaemic HF-REF, discriminating cases from controls both as single markers or when combined in a multivariate signature. In addition, we demonstrate that the miRNAs of this signature significantly correlate with disease severity as indicated by left ventricular ejection fraction.
  
  
  CONCLUSIONS
  Our data further denote that miRNAs are potential biomarkers for systolic heart failure. Since their detection levels in whole blood are also related to the degree of left ventricular dysfunction, they may serve as objective molecular tools to assess disease severity and prognosis.
authors.affiliation: Department of Internal Medicine III, University of Heidelberg, Im Neuenheimer Feld 410, 69120  Heidelberg, Germany.
authors.forename: Britta
authors.initials: B
authors.lastname: Vogel
authors.forename: Andreas
authors.initials: A
authors.lastname: Keller
authors.forename: Karen S
authors.initials: KS
authors.lastname: Frese
authors.forename: Petra
authors.initials: P
authors.lastname: Leidinger
authors.forename: Farbod
authors.initials: F
authors.lastname: Sedaghat-Hamedani
authors.forename: Elham
authors.initials: E
authors.lastname: Kayvanpour
authors.forename: Wanda
authors.initials: W
authors.lastname: Kloos
authors.forename: Christina
authors.initials: C
authors.lastname: Backe
authors.forename: Ann
authors.initials: A
authors.lastname: Thanaraj
authors.forename: Thomas
authors.initials: T
authors.lastname: Brefort
authors.forename: Markus
authors.initials: M
authors.lastname: Beier
authors.forename: Stefan
authors.initials: S
authors.lastname: Hardt
authors.forename: Eckart
authors.initials: E
authors.lastname: Meese
authors.forename: Hugo A
authors.initials: HA
authors.lastname: Katus
authors.forename: Benjamin
authors.initials: B
authors.lastname: Meder
mesh_terms: D015415:Biomarkers / Q000378:metabolism; D016022:Case-Control Studies; D005260:Female; D055106:Genome-Wide Association Study; D054143:Heart Failure, Systolic / Q000175:diagnosis* / Q000503:physiopathology; D006801:Humans; D053208:Kaplan-Meier Estimate; D008297:Male; D035683:MicroRNAs / Q000378:metabolism*; D008875:Middle Aged; D020097:Natriuretic Peptide, Brain / Q000378:metabolism; D010446:Peptide Fragments / Q000378:metabolism; D013318:Stroke Volume; D018487:Ventricular Dysfunction, Left / Q000175:diagnosis / Q000503:physiopathology
chemical_list: D015415:Biomarkers; D035683:MicroRNAs; D010446:Peptide Fragments; C109794:pro-brain natriuretic peptide (1-76); D020097:Natriuretic Peptide, Brain
keywords: Biomarker; HF-REF; Non-ischaemic heart failure; miRNA