title: Pattern reorganization occurs independently of cell division during Drosophila wing disc regeneration in situ.
abstract: One of the most intriguing problems in developmental biology is how an organism can replace missing organs or portions of its body after injury. This capacity, known as regeneration, is conserved across different phyla. The imaginal discs of Drosophila melanogaster provide a particularly well-characterized model for analyzing regeneration. We have developed a unique method to study organ regeneration under physiological conditions using the imaginal discs of Drosophila. Using this method, we revisited different aspects of organ regeneration. The results presented in this report suggest that during the initial stages of regeneration, different processes occur, including wound healing, a temporary loss of markers of cell-fate commitment, and pattern reorganization. We present evidence indicating that all of these processes occur even when cell division has been arrested. Our data also suggested that Wingless is not required during the early stages of disc regeneration.
authors.affiliation: Centro de Biología Molecular Severo Ochoa, Universidad Autónoma de Madrid, Consejo Superior de Investigaciones Científicas (CSIC), Madrid 28049, Spain.
authors.forename: Sandra
authors.initials: S
authors.lastname: Díaz-García
authors.forename: Antonio
authors.initials: A
authors.lastname: Baonza
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D002455:Cell Division / Q000502:physiology; D049109:Cell Proliferation; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism; D004331:Drosophila melanogaster / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D060227:Imaginal Discs / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D007150:Immunohistochemistry; D008856:Microscopy, Fluorescence; D008954:Models, Biological; D034622:RNA Interference; D012038:Regeneration / Q000235:genetics / Q000502:physiology*; D013997:Time Factors; D014921:Wings, Animal / Q000293:injuries / Q000378:metabolism / Q000503:physiopathology*; D051155:Wnt1 Protein / Q000235:genetics / Q000378:metabolism; D014945:Wound Healing / Q000235:genetics / Q000502:physiology*
chemical_list: D029721:Drosophila Proteins; D051155:Wnt1 Protein; C074622:wg protein, Drosophila; D049452:Green Fluorescent Proteins
keywords: epimorphic; morphallatic