title: An evaluation of setting time of mineral trioxide aggregate and EndoSequence root repair material in the presence of human blood and minimal essential media.
abstract: BACKGROUND
  The purpose of this study was to compare the ability of MTA (Dentsply Maillefer, Tulsa, OK) and EndoSequence Root Repair Material (ESRRM; Brasseler USA, Savannah, GA) to set in the presence of human blood and minimal essential media.
  
  
  METHODS
  A model was created using polymethyl methacrylate blocks each prepared with 10 standardized wells (2-mm diameter × 3-mm depth). Prepared ProRoot MTA (Dentsply Maillefer) and ESRRM were each placed in 6 separate blocks. The samples were distributed among the 4 different media (ie, blood, minimal essential media, blood and minimal essential media, and sterile saline as the control). Each block was submerged for 4, 5, 6, 8, 24, 36, and 48 hours in an incubator at 37°C with 100% humidity.
  
  
  RESULTS
  The results revealed that regardless of the type of media exposure, neither of the materials set at 4 or 6 hours. ESRRM was not set at 48 hours, whereas all of the MTA samples were set at 36 hours.
  
  
  CONCLUSIONS
  This outcome draws into question the proposed setting time given by each respective manufacturer. Furthermore, despite ESRRM being marketed as a direct competitor to MTA with superior handling properties, MTA consistently set at a faster rate under the conditions of this study.
authors.affiliation: University of Medicine and Dentistry of New Jersey, Newark, NJ 07101-1709, USA. charland.dmd@gmail.com
authors.forename: Trisha
authors.initials: T
authors.lastname: Charland
authors.forename: Gary R
authors.initials: GR
authors.lastname: Hartwell
authors.forename: Craig
authors.initials: C
authors.lastname: Hirschberg
authors.forename: Reena
authors.initials: R
authors.lastname: Patel
mesh_terms: D017607:Aluminum Compounds / Q000737:chemistry*; D001769:Blood; D017610:Calcium Compounds / Q000737:chemistry*; D002130:Calcium Phosphates / Q000737:chemistry*; D055598:Chemical Phenomena; D003470:Culture Media; D004338:Drug Combinations; D006801:Humans; D006813:Humidity; D008422:Materials Testing; D010087:Oxides / Q000737:chemistry*; D044367:Phase Transition; D012387:Root Canal Filling Materials / Q000737:chemistry*; D017640:Silicates / Q000737:chemistry*; D012965:Sodium Chloride / Q000737:chemistry; D013635:Tantalum / Q000737:chemistry*; D013696:Temperature; D013997:Time Factors; D015040:Zirconium / Q000737:chemistry*
chemical_list: D017607:Aluminum Compounds; D017610:Calcium Compounds; D002130:Calcium Phosphates; D003470:Culture Media; D004338:Drug Combinations; C550603:EndoSequence root repair material; D010087:Oxides; D012387:Root Canal Filling Materials; D017640:Silicates; C086631:mineral trioxide aggregate; D012965:Sodium Chloride; D013635:Tantalum; D015040:Zirconium
keywords: MTA; Messing gun; Micro-Apical Placement System; peri-apical surgery