title: LC-ESI-MS/MS analysis and pharmacokinetics of jolkinolide B, a potential antitumor active component isolated from Euphorbia fischeriana, in rat plasma.
abstract: A simple, specific and reproducible liquid chromatography-electrospray ionization mass spectrometry was developed and validated for the determination of jolkinolide B, a potential antitumor active component isolated from Euphorbia fischeriana, in rat plasma. Chromatographic separation was achieved on a Venusil MP-C18 column using an isocratic elution. Jolkinolide B and osthole (internal standard) were monitored by positive electrospray ionization in the selected reaction monitoring mode. Good linearity (r(2) > 0.996) was achieved by a weighted (1/x(2) ) linear least-squares regression over a concentration range of 6.50-2600 ng/mL. The accuracy and precision of the assay were satisfactory and the method proved to be applicable to pharmacokinetics following a single intravenous bolus injection of jolkinolide B to rats.
authors.affiliation: Department of Breast Surgery, Liaoning Cancer Hospital and Institute, Shenyang, 110042, People's Republic of China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Li
authors.forename: Xiao-Li
authors.initials: XL
authors.lastname: Liu
authors.forename: Zhen-Gang
authors.initials: ZG
authors.lastname: Cai
authors.forename: Si-Xi
authors.initials: SX
authors.lastname: Zhang
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics*; D002853:Chromatography, Liquid / Q000379:methods; D004224:Diterpenes / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics*; D004355:Drug Stability; D028481:Euphorbia / Q000737:chemistry*; D016018:Least-Squares Analysis; D008297:Male; D010936:Plant Extracts / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000379:methods
chemical_list: D000970:Antineoplastic Agents; D004224:Diterpenes; D010936:Plant Extracts; C057914:jolkinolide B
keywords: LC-ESI-MS/MS; antitumor component; jolkinolide B; pharmacokinetics; rat plasma