title: Use of cell-free circulating schistosome DNA in serum, urine, semen, and saliva to monitor a case of refractory imported schistosomiasis hematobia.
abstract: This case of imported refractory schistosomiasis has highlighted the usefulness of cell-free parasite DNA as a diagnostic marker to assess active schistosome infection. In contrast to the rapid disappearance of ova in urine, parasite DNA remained persistent in several other specimen types even after the fourth treatment with praziquantel. This result was consistent with the presence of morphologically intact ova in bladder biopsy samples and with the corresponding symptoms.
authors.affiliation: Laboratory of Tropical Medicine and Parasitology, Dokkyo Medical University, Tochigi, Japan.
authors.forename: Naoko
authors.initials: N
authors.lastname: Kato-Hayashi
authors.forename: Mitsuko
authors.initials: M
authors.lastname: Yasuda
authors.forename: Jozi
authors.initials: J
authors.lastname: Yuasa
authors.forename: Shigeo
authors.initials: S
authors.lastname: Isaka
authors.forename: Kosuke
authors.initials: K
authors.lastname: Haruki
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ohmae
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Osada
authors.forename: Tamotsu
authors.initials: T
authors.lastname: Kanazawa
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Chigusa
mesh_terms: D000818:Animals; D000871:Anthelmintics / Q000627:therapeutic use; D001706:Biopsy; D018509:DNA, Helminth / Q000235:genetics / Q000302:isolation & purification*; D016903:Drug Monitoring / Q000379:methods*; D006801:Humans; D008297:Male; D010274:Parasitology / Q000379:methods*; D011223:Praziquantel / Q000627:therapeutic use; D012463:Saliva / Q000469:parasitology; D012547:Schistosoma / Q000302:isolation & purification; D012552:Schistosomiasis / Q000175:diagnosis* / Q000188:drug therapy / Q000469:parasitology*; D012661:Semen / Q000469:parasitology; D044967:Serum / Q000469:parasitology; D001743:Urinary Bladder / Q000469:parasitology; D014556:Urine / Q000469:parasitology; D055815:Young Adult
chemical_list: D000871:Anthelmintics; D018509:DNA, Helminth; D011223:Praziquantel