title: A novel TaqI polymorphism in the coding region of the ovine TNXB gene in the MHC class III region: morphostructural and physiological influences.
abstract: The tenascin-XB (TNXB) gene has antiadhesive effects, functions in matrix maturation in connective tissues, and localizes to the major histocompatibility complex class III region. We hypothesized that it may influence adaptive physiological response through an effect on blood vessel function. We identified a novel g.1324 A→G polymorphism at a TaqI recognition site in a 454 bp fragment of ovine TNXB and genotyped it in 150 Nigerian sheep using PCR-RFLP. The missense mutation changes glutamic acid (GAA) to glycine (GGA). Among SNP genotypes, significant differences (P < 0.05) were observed in body weight and fore cannon bone length. Interaction effects of breed, SNP genotype, and geographic location had a significant effect (P < 0.05) on chest girth. The SNP genotype was significantly (P < 0.05) associated with physiological traits of pulse rate and skin temperature. The observed effect of this novel polymorphism may be mediated through its role in connective tissue biology, requiring further association and functional studies.
authors.affiliation: Department of Animal Breeding and Genetics, University of Agriculture, Abeokuta, Nigeria.
authors.forename: Oyeyemi O
authors.initials: OO
authors.lastname: Ajayi
authors.forename: Mufliat A
authors.initials: MA
authors.lastname: Adefenwa
authors.forename: Brilliant O
authors.initials: BO
authors.lastname: Agaviezor
authors.forename: Christian O N
authors.initials: CO
authors.lastname: Ikeobi
authors.forename: Matthew
authors.initials: M
authors.lastname: Wheto
authors.forename: Moses
authors.initials: M
authors.lastname: Okpeku
authors.forename: Samuel A
authors.initials: SA
authors.lastname: Amusan
authors.forename: Abdulmojeed
authors.initials: A
authors.lastname: Yakubu
authors.forename: Marcos
authors.initials: M
authors.lastname: De Donato
authors.forename: Sunday O
authors.initials: SO
authors.lastname: Peters
authors.forename: Ikhide G
authors.initials: IG
authors.lastname: Imumorin
mesh_terms: D000818:Animals; D001831:Body Temperature; D001835:Body Weight / Q000235:genetics; D001842:Bone and Bones / Q000033:anatomy & histology; D015252:Deoxyribonucleases, Type II Site-Specific / Q000235:genetics*; D005838:Genotype; D008285:Major Histocompatibility Complex; D009549:Nigeria; D020641:Polymorphism, Single Nucleotide; D011674:Pulse; D034561:Sheep, Domestic / Q000033:anatomy & histology / Q000235:genetics* / Q000502:physiology; D012879:Skin Physiological Phenomena; D019063:Tenascin / Q000235:genetics*
chemical_list: D019063:Tenascin; C081865:tenascin X; D015252:Deoxyribonucleases, Type II Site-Specific; C027046:TCGA-specific type II deoxyribonucleases