title: Dynamic expressions of Beclin 1 and tyrosine hydroxylase in different areas of 6-hydroxydopamine-induced Parkinsonian rats.
abstract: Beclin 1, a regulator of the autophagy pathway, plays an important role in Parkinson's disease (PD). However, the crucial mechanism of Beclin 1 in PD remains unclear. Therefore, we investigated dynamic expressions of Beclin 1 and tyrosine hydroxylase (TH) in different brain areas of 6-OHDA-induced rats. Beclin 1 and TH expressions were analyzed by flow cytometry and immunohistochemistry, respectively. The results showed that Beclin 1 expressions were low in the sham group, but rose significantly after 6-OHDA injection. In the striatum and cortex, Beclin 1 increased at 3 h, peaking at 12 h, while in the hippocampus, it increased at 3 h and peaked at 24 h, then it declined slowly and remained steady at 72 h. Beclin 1 expression in the striatum and cortex areas was higher than that of the hippocampus area at 12 h. In addition, the time-course of TH expression in the striatum was similar to that in the mesencephalon. TH expression declined dramatically between 0 and 12 h. Pearson analysis showed significant negative correlations between TH and Beclin 1 expression in the areas we analyzed. While TH expression declined gradually between 12 and 72 h, significant positive correlations between TH and Beclin 1 were detected during that interval. This indicated that activation of Beclin 1-dependent autophagy may inhibit the loss of TH-positive neurons.
authors.affiliation: The First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, 510405, China.
authors.forename: Sheng
authors.initials: S
authors.lastname: Zhang
authors.forename: Zhong-Feng
authors.initials: ZF
authors.lastname: Xue
authors.forename: Li-Ping
authors.initials: LP
authors.lastname: Huang
authors.forename: Ruo-Ming
authors.initials: RM
authors.lastname: Fang
authors.forename: Yu-Ping
authors.initials: YP
authors.lastname: He
authors.forename: Ling
authors.initials: L
authors.lastname: Li
authors.forename: Yong-Qi
authors.initials: YQ
authors.lastname: Fang
mesh_terms: D000818:Animals; D051017:Apoptosis Regulatory Proteins / Q000378:metabolism*; D000071186:Beclin-1; D001921:Brain / Q000201:enzymology* / Q000473:pathology*; D005260:Female; D005434:Flow Cytometry; D007150:Immunohistochemistry; D007267:Injections; D009928:Organ Specificity; D016627:Oxidopamine; D010300:Parkinson Disease / Q000201:enzymology* / Q000473:pathology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism*
chemical_list: D051017:Apoptosis Regulatory Proteins; D000071186:Beclin-1; C512829:Becn1 protein, rat; D016627:Oxidopamine; D014446:Tyrosine 3-Monooxygenase