title: The presence of RANKL-OPG complex in human osteosarcoma U2OS.
abstract: A sensitive sandwich enzyme-linked immunosorbent assay (ELISA) for the human receptor activator of nuclear factor κB (RANKL)-osteoprotegerin (OPG) complex was developed by utilizing a monoclonal antibody that recognizes human soluble RANKL as an immobilized capture component and biotinylated human OPG polyclonal antibody. We could quantify the RANKL-OPG complex level (detection limit: 100 pg/mL). Employing this assay system, we demonstrated that the RANKL-OPG complex was constitutively present in the conditioned medium of human osteoblastic sarcoma U2OS, although the complex was not detectable in other human osteoblastic sarcoma cell line, MG-63, HOS, and SaOS-2.
authors.affiliation: Department of Medicinal Biochemistry, School of Pharmacy, Aichi-Gakuin University, Nagoya, Japan. agumm7@yahoo.co.jp
authors.forename: Makio
authors.initials: M
authors.lastname: Mogi
authors.forename: Ayami
authors.initials: A
authors.lastname: Kondo
mesh_terms: D045744:Cell Line, Tumor; D017077:Culture Media, Conditioned; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006801:Humans; D053244:Osteoprotegerin / Q000096:biosynthesis*; D012516:Osteosarcoma / Q000276:immunology / Q000378:metabolism*; D053245:RANK Ligand / Q000096:biosynthesis*
chemical_list: D017077:Culture Media, Conditioned; D053244:Osteoprotegerin; D053245:RANK Ligand