title: The lectin of Dolichos biflorus agglutinin recognises glycan epitopes on the surface of a subset of cardiac progenitor cells.
abstract: The discovery of adult cardiac progenitor cells (CPCs) provides a promising way for treating heart disease; however, their surface characteristics that play a critical role in regulating their maintenance, self-renewal, migration, and differentiation have not been fully investigated. One subpopulation of Dolichos biflorus agglutinin (DBA)-positive cells was identified in the heart of adult mice. Flow cytometry showed that 3.7% of heart cells could be labeled by FITC conjugated DBA. BrdU pulse-chase showed that 55-75% of DBA(+) cells were CPCs. Evidences from 5-FU-induced myelosuppression along with BrdU pulse-chasing suggests that DBA-positive cells are proliferative. Furthermore, DBA positive cells display a cologenic appearance in vivo. Our findings suggest that DBA-positive cells in the heart of adult mouse contained a subset of CPCs, and DBA reactivity is one novel surface characteristic on CPCs.
authors.affiliation: School of Public Health, Wuhan University, Wuhan, 430071, China; Wuhan Medical Health Center for Women and Children, Wuhan, 430016, China.
authors.forename: Zhanfeng
authors.initials: Z
authors.lastname: Chen
authors.forename: Man
authors.initials: M
authors.lastname: Wang
authors.forename: Qiang
authors.initials: Q
authors.lastname: Xiang
authors.forename: Zhenliang
authors.initials: Z
authors.lastname: Sun
authors.forename: Rong
authors.initials: R
authors.lastname: Zhang
mesh_terms: D000818:Animals; D001973:Bromodeoxyuridine / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects; D002999:Clone Cells; D000939:Epitopes / Q000276:immunology*; D005472:Fluorouracil / Q000494:pharmacology; D051379:Mice; D008813:Mice, Inbred ICR; D009206:Myocardium / Q000166:cytology*; D037121:Plant Lectins / Q000276:immunology*; D011134:Polysaccharides / Q000276:immunology*; D019009:Proto-Oncogene Proteins c-kit / Q000378:metabolism; D013194:Staining and Labeling; D013234:Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000276:immunology*
chemical_list: D000939:Epitopes; D037121:Plant Lectins; D011134:Polysaccharides; C036554:dolichos biflorus agglutinin; D019009:Proto-Oncogene Proteins c-kit; D001973:Bromodeoxyuridine; D005472:Fluorouracil
keywords: BrdU pulse chasing; Dolichos biflorus agglutinin; cardiac progenitor cells; niche