title: Detection of the common resistance genes in Gram-negative bacteria using gene chip technology.
abstract: OBJECTIVE
  To design a resistance gene detection chip that could, in parallel, detect common clinical drug resistance genes of Gram-negative bacteria.
  
  
  METHODS
  Seventy clinically significant Gram-negative bacilli (Klebsiella pneumoniae, Escherichia coli, Enterobacter cloacae, Pseudomonas aeruginosa, Acinetobacter baumannii) were collected. According to the known resistance gene sequences, we designed and synthesized primers and probes, which were used to prepare resistance gene detection chips, and finally we hybridized and scanned the gene detection chips.
  
  
  RESULTS
  The results between the gene chip and polymerase chain reaction (PCR) were compared. The rate was consistently 100% in the eight kinds of resistance genes tested (TEM, SHV, CTX-M, DHA, CIT, VIM, KPC, OXA-23). One strain of Pseudomonas aeruginosa had the IMP, but it was not found by gene chip.
  
  
  CONCLUSIONS
  The design of Gram-negative bacteria-resistant gene detection chip had better application value.
authors.affiliation: Clinical Research Center of Ningbo No. 2 Hospital, Ningbo, Zhejiang, 315010, China.
authors.forename: C
authors.initials: C
authors.lastname: Ting
authors.forename: A
authors.initials: A
authors.lastname: Jun
authors.forename: Z
authors.initials: Z
authors.lastname: Shun
mesh_terms: D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D024881:Drug Resistance, Bacterial; D006090:Gram-Negative Bacteria / Q000187:drug effects / Q000235:genetics*; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D015345:Oligonucleotide Probes / Q000235:genetics; D012680:Sensitivity and Specificity
chemical_list: D017931:DNA Primers; D004269:DNA, Bacterial; D015345:Oligonucleotide Probes