title: Acquisition of thin coronal sectional dataset of cadaveric liver.
abstract: OBJECTIVE
  To obtain the thin coronal sectional anatomic dataset of the liver by using digital freezing milling technique.
  
  
  METHODS
  The upper abdomen of one Chinese adult cadaver was selected as the specimen. After CT and MRI examinations verification of absent liver lesions, the specimen was embedded with gelatin in stand erect position and frozen under profound hypothermia, and the specimen was then serially sectioned from anterior to posterior layer by layer with digital milling machine in the freezing chamber. The sequential images were captured by means of a digital camera and the dataset was imported to imaging workstation.
  
  
  RESULTS
  The thin serial section of the liver added up to 699 layers with each layer being 0.2 mm in thickness. The shape, location, structure, intrahepatic vessels and adjacent structures of the liver was displayed clearly on each layer of the coronal sectional slice. CT and MR images through the body were obtained at 1.0 and 3.0 mm intervals, respectively.
  
  
  CONCLUSIONS
  The methodology reported here is an adaptation of the milling methods previously described, which is a new data acquisition method for sectional anatomy. The thin coronal sectional anatomic dataset of the liver obtained by this technique is of high precision and good quality.
authors.affiliation: Research Center for Sectional and Imaging Anatomy, School of Medicine, Shandong University, 44#, Wenhua Xi Road, Jinan, 250012, Shandong, People's Republic of China.
authors.forename: Li
authors.initials: L
authors.lastname: Lou
authors.forename: Shu Wei
authors.initials: SW
authors.lastname: Liu
authors.forename: Zhen Mei
authors.initials: ZM
authors.lastname: Zhao
authors.forename: Yu Chun
authors.initials: YC
authors.lastname: Tang
authors.forename: Xiang Tao
authors.initials: XT
authors.lastname: Lin
mesh_terms: D000328:Adult; D002102:Cadaver; D066264:Datasets as Topic; D005615:Freezing; D006652:Histological Techniques / Q000379:methods*; D006801:Humans; D008099:Liver / Q000033:anatomy & histology*; D008279:Magnetic Resonance Imaging; D008297:Male; D008867:Microtomy; D010781:Photography; D014057:Tomography, X-Ray Computed