title: Neuroprotective effects of madecassoside in early stage of Parkinson's disease induced by MPTP in rats.
abstract: In this study, we investigated the neuroprotective effects of madecassoside, isolated from the Chinese medicinal herb Centella asiatica, in the rat model of early phase of parkinsonism. During intragastric administrations of madecassoside for 7 days, the rats were injected with MPTP on the 7th day. And for the following 14 days, madecassoside were also administered. On the 14th day, the behavioral tests were assessed after 1h of administration. And then, the rats were sacrificed, substantia nigra and striatum were dissected. The content of DA, MDA, GSH, and Bcl-2/Bax gene expression levels and BDNF protein level was determined. Treatment with madecassoside was found to improve locomotor dysfunction and to protect dopaminergic neuron by antagonizing MPTP induced neurotoxicity. Madecassoside significantly attenuated the MPTP-induced reduction of dopamine in the striatum. The MDA contents were significantly decreased while the GSH levels, Bcl-2/Bax ratio and protein expression of BDNF were significantly increased in madecassoside treated groups. These results indicated that madecassoside was effective in recovering MPTP-induced early signs of parkinsonism via its neuroprotective effects including reversing the depletion of DA, antioxidant activity, increasing ratio of Bcl-2/Bax, increasing protein expression of BDNF.
authors.affiliation: Department of Pharmacology of Traditional Chinese Medical Formulae, Nanjing University of Traditional Chinese Medicine, Nanjing 210029, PR China; Department of Pharmacology of Chinese Materia Medica, China Pharmaceutical University, Nanjing 210009, PR China.
authors.forename: Chang-Liang
authors.initials: CL
authors.lastname: Xu
authors.forename: Rong
authors.initials: R
authors.lastname: Qu
authors.forename: Jin
authors.initials: J
authors.lastname: Zhang
authors.forename: Lu-Fan
authors.initials: LF
authors.lastname: Li
authors.forename: Shi-Ping
authors.initials: SP
authors.lastname: Ma
mesh_terms: D015632:1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine; D000818:Animals; D000975:Antioxidants / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D001921:Brain / Q000187:drug effects* / Q000378:metabolism; D019208:Brain-Derived Neurotrophic Factor / Q000378:metabolism; D028041:Centella / Q000737:chemistry*; D003342:Corpus Striatum / Q000187:drug effects / Q000378:metabolism; D004298:Dopamine / Q000378:metabolism; D059290:Dopaminergic Neurons / Q000187:drug effects / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D008124:Locomotion / Q000187:drug effects; D020267:MPTP Poisoning / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D018696:Neuroprotective Agents / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D008517:Phytotherapy; D010936:Plant Extracts; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013378:Substantia Nigra / Q000187:drug effects / Q000378:metabolism; D014315:Triterpenes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*
chemical_list: D000975:Antioxidants; D019208:Brain-Derived Neurotrophic Factor; C526146:Centella asiatica extract; D018696:Neuroprotective Agents; D010936:Plant Extracts; D019253:Proto-Oncogene Proteins c-bcl-2; D014315:Triterpenes; D008315:Malondialdehyde; D015632:1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine; C093443:madecassoside; D005978:Glutathione; D004298:Dopamine
keywords: 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine; 3 4-dihydroxybenzylamine; 3 4-dihydroxyphenylacetic acid; BDNF; Bcl-2/Bax ratio; DA; DHBA; DOPAC; GSH; HPLC; HVA; LPO; MDA; MPTP; Madecassoside; PD; Parkinson's disease; Redox balance; SN; TBA; TBARS; brain derived neurophic factor; dopamine; high-performance liquid chromatography; homovanillic acid; lipid peroxidation; malonyldialdehyde; qRT-PCR; quantitative real-time polymerase chain reaction; reduced glutathione; substantia nigra; thiobarbituric acid; thiobarbituric acid reactive substances