title: Simultaneous determination of atropine, scopolamine, and anisodamine in Flos daturae by capillary electrophoresis using a capillary coated by graphene oxide.
abstract: A novel CE method was developed for the separation and determination of three main tropane alkaloids in Flos daturae with a capillary coated by graphene oxide (GO). The GO-coated capillary was characterized by SEM, energy dispersive X-ray spectroscopy, and Raman spectroscopy, and the results indicated that the inner surface of the capillary was partially coated by GO. A phosphate solution (40 mM, pH7.0) containing 20% v/v methanol and 30% v/v acetonitrile was used as the running buffer for the analysis of the atropine, scopolamine, and anisodamine. The linear ranges of atropine, scopolamine, and anisodamine was 0.5-200 μg/mL with satisfactory correlation coefficients (R(2)) > 0.9987, and this novel method provided an efficient separation for three tropane alkaloids as well as a good reproducibility and stability. Finally, the method was successfully applied for the determination of these three tropane alkaloids in plant extracts.
authors.affiliation: Department of Chemistry, Capital Normal University, Beijing, PR China. yenshcnu@126.com
authors.forename: Nengsheng
authors.initials: N
authors.lastname: Ye
authors.forename: Jian
authors.initials: J
authors.lastname: Li
authors.forename: Chong
authors.initials: C
authors.lastname: Gao
authors.forename: Yali
authors.initials: Y
authors.lastname: Xie
mesh_terms: D001285:Atropine / Q000737:chemistry*; D032302:Datura / Q000737:chemistry*; D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods*; D035264:Flowers / Q000737:chemistry; D006108:Graphite / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry*; D012601:Scopolamine / Q000737:chemistry*; D012991:Solanaceous Alkaloids / Q000737:chemistry*
chemical_list: D010936:Plant Extracts; D012991:Solanaceous Alkaloids; C003922:anisodamine; D006108:Graphite; D001285:Atropine; D012601:Scopolamine
keywords: Anisodamine; Atropine; CE; Graphene oxide; Scopolamine