title: Nondestructive assessment of current one-step self-etch dental adhesives using optical coherence tomography.
abstract: ABSTRACT. This study aimed to nondestructively evaluate sealing performance of eight one-step self-etch adhesives (1-SEAs) using optical coherence tomography (OCT). The two-step self-etch adhesive (2-SEA) served as the control. Round tapered class-I cavities (D=4 mm, H=2 mm) were prepared in bovine incisors, treated with each adhesive (n=5), and restored with a flowable resin composite. Cross-sections were obtained from each restoration using swept-source OCT with 1310-nm laser. The average percentage of the sealed interface (SI%) for each adhesive was calculated using image analysis software, considering increased signal intensity at the interface as gap. Samples were then sectioned and observed under confocal laser scanning microscope (CLSM). Significantly different SI% values were found among different adhesives (analysis of variance, Bonferroni, p<0.05). There was also a significant correlation in SI% between OCT and CLSM (p<0.0001, r=0.96). Additionally, microscopic analysis revealed that the gaps in 1-SEAs occurred not only at dentine-adhesive interfaces but also frequently at adhesive-composite interfaces. Some recent 1-SEAs could achieve reliable short-term sealing comparable to 2-SEA. OCT is a unique tool to nondestructively evaluate the sealing performance of the restoratives through the cavity, provided that cavity walls have a certain minimum inclination with respect to the beam.
authors.affiliation: Tokyo Medical and Dental University, Cariology and Operative Dentistry, Department of Oral Health Sciences and Global Center of Excellence Program, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8549, Japan.
authors.forename: Baba
authors.initials: B
authors.lastname: Bista
authors.forename: Alireza
authors.initials: A
authors.lastname: Sadr
authors.forename: Amir
authors.initials: A
authors.lastname: Nazari
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Shimada
authors.forename: Yasunori
authors.initials: Y
authors.lastname: Sumi
authors.forename: Junji
authors.initials: J
authors.lastname: Tagami
mesh_terms: D000818:Animals; D002417:Cattle; D003738:Dental Cements / Q000737:chemistry*; D003743:Dental Enamel / Q000737:chemistry; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D007180:Incisor / Q000737:chemistry; D008422:Materials Testing; D018613:Microscopy, Confocal; D012031:Refractometry; D012117:Resins, Synthetic; D041623:Tomography, Optical Coherence / Q000379:methods*
chemical_list: D003738:Dental Cements; D012117:Resins, Synthetic