title: P450 in wild animals as a biomarker of environmental impact.
abstract: The impact of environmental pollution on selected animals was tested by monitoring the hepatic content of cytochromes P450 and their enzyme activities or by calculating TEQ values from the concentration of pollutants in the body. Fish-eating Stellars Sea Eagles, Haliaeetus pelagicus, found dead in the northern part of Hokkaido island accumulated high levels of PCBs and DDT and metabolites. The TEQ values calculated from the PCB concentration in the eagles were high enough to cause a significant toxic effect in other birds living in the same environment. Some of these birds were also contaminated with high concentrations of lead. Spotted seals, Phoca largha, captured along the coast-line of Hokkaido accumulated PCBs in their fat at about 100 million times the concentrations in the surface sea water. The levels of expressions of hepatic microsomal CYP 1A1and related enzyme activities in these seals showed good correlation to the levels of PCBs accumulated in the fat. The fresh water crabs, Eriocheir japonicus, were captured from three different rivers with various degrees of pollution. The P450 content and the related enzyme activities showed good correlation to TEQ values obtained from the concentrations of PCBs and PCDDs in the crabs from the rivers. The wild rodents, Clethrionomys rufocanus, were captured from urban, agricultural, and forest areas in Hokkaido. Those from the forest area had the lowest CYP content and related enzyme activities, comparable to those in laboratory-raised animals. Those from the urban areas, presumably contaminated with PAHs from fuel combustion, showed increased CYP 1A1 content and related enzyme activities. Those from the agricultural areas showed increased levels of CYP 1A1, 2B, 2E1. Rats treated with some of the agrochemicals used in the area resulted in a similar pattern of induction. It is concluded that P450 can be a useful biomarker for assessing the environmental impact of chemical pollutants on wild animals.
authors.affiliation: Department of Environmental Veterinary Sciences, Graduate School of Veterinary Medicine, Hokkaido University,Sapporo, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Fujita
authors.forename: I
authors.initials: I
authors.lastname: Chiba
authors.forename: M
authors.initials: M
authors.lastname: Ishizuka
authors.forename: H
authors.initials: H
authors.lastname: Hoshi
authors.forename: H
authors.initials: H
authors.lastname: Iwata
authors.forename: A
authors.initials: A
authors.lastname: Sakakibara
authors.forename: S
authors.initials: S
authors.lastname: Tanabe
authors.forename: A
authors.initials: A
authors.lastname: Kazusaka
authors.forename: M
authors.initials: M
authors.lastname: Masuda
authors.forename: Y
authors.initials: Y
authors.lastname: Masuda
authors.forename: H
authors.initials: H
authors.lastname: Nakagawa