title: Unlipidated outer membrane protein Omp16 (U-Omp16) from Brucella spp. as nasal adjuvant induces a Th1 immune response and modulates the Th2 allergic response to cow's milk proteins.
abstract: The discovery of novel mucosal adjuvants will help to develop new formulations to control infectious and allergic diseases. In this work we demonstrate that U-Omp16 from Brucella spp. delivered by the nasal route (i.n.) induced an inflammatory immune response in bronchoalveolar lavage (BAL) and lung tissues. Nasal co-administration of U-Omp16 with the model antigen (Ag) ovalbumin (OVA) increased the amount of Ag in lung tissues and induced OVA-specific systemic IgG and T helper (Th) 1 immune responses. The usefulness of U-Omp16 was also assessed in a mouse model of food allergy. U-Omp16 i.n. administration during sensitization ameliorated the hypersensitivity responses of sensitized mice upon oral exposure to Cow's Milk Protein (CMP), decreased clinical signs, reduced anti-CMP IgE serum antibodies and modulated the Th2 response in favor of Th1 immunity. Thus, U-Omp16 could be used as a broad Th1 mucosal adjuvant for different Ag formulations.
authors.affiliation: Laboratorio de Inmunogenética, INIGEM-CONICET, Hospital de Clínicas José de San Martín, Facultad de Medicina, Universidad de Buenos Aires (UBA), Buenos Aires, Argentina.
authors.forename: Andrés E
authors.initials: AE
authors.lastname: Ibañez
authors.forename: Paola
authors.initials: P
authors.lastname: Smaldini
authors.forename: Lorena M
authors.initials: LM
authors.lastname: Coria
authors.forename: María V
authors.initials: MV
authors.lastname: Delpino
authors.forename: Lucila G G
authors.initials: LG
authors.lastname: Pacífico
authors.forename: Sergio C
authors.initials: SC
authors.lastname: Oliveira
authors.forename: Gabriela S
authors.initials: GS
authors.lastname: Risso
authors.forename: Karina A
authors.initials: KA
authors.lastname: Pasquevich
authors.forename: Carlos Alberto
authors.initials: CA
authors.lastname: Fossati
authors.forename: Guillermo H
authors.initials: GH
authors.lastname: Giambartolomei
authors.forename: Guillermo H
authors.initials: GH
authors.lastname: Docena
authors.forename: Juliana
authors.initials: J
authors.lastname: Cassataro
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage; D000818:Animals; D000941:Antigens / Q000276:immunology / Q000378:metabolism; D001425:Bacterial Outer Membrane Proteins / Q000008:administration & dosage / Q000737:chemistry / Q000276:immunology*; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology / Q000276:immunology; D002002:Brucella / Q000276:immunology*; D002417:Cattle; D002490:Central Nervous System / Q000276:immunology / Q000473:pathology; D016207:Cytokines / Q000096:biosynthesis; D004195:Disease Models, Animal; D005260:Female; D007073:Immunoglobulin E / Q000276:immunology; D007074:Immunoglobulin G / Q000276:immunology; D008168:Lung / Q000276:immunology / Q000473:pathology; D051379:Mice; D016269:Milk Hypersensitivity / Q000276:immunology* / Q000378:metabolism; D008894:Milk Proteins / Q000276:immunology*; D009297:Nasal Mucosa / Q000276:immunology / Q000378:metabolism; D013154:Spleen / Q000276:immunology; D018417:Th1 Cells / Q000276:immunology* / Q000378:metabolism; D018418:Th2 Cells / Q000276:immunology* / Q000378:metabolism
chemical_list: D000276:Adjuvants, Immunologic; D000941:Antigens; D001425:Bacterial Outer Membrane Proteins; D016207:Cytokines; D007074:Immunoglobulin G; D008894:Milk Proteins; D007073:Immunoglobulin E