title: Proteomic and properties analysis of botanical insecticide rhodojaponin III-induced response of the diamondback moth, Plutella xyllostella (L.).
abstract: BACKGROUND
  Rhodojaponin III, as a botanical insecticide, affects a wide variety of biological processes in insects, including reduction of feeding, suspension of development, and oviposition deterring of adults in a dose-dependent manner. However, the mode of these actions remains obscure.
  
  
  RESULTS
  In this study, a comparative proteomic approach was adopted to examine the effect of rhodojaponin III on the Plutella xyllostella (L.). Following treating 48 hours, newly emergence moths were collected and protein samples were prepared. The proteins were separated by 2-DE, and total 31 proteins were significantly affected by rhodojaponin III compared to the control identified by MALDI-TOF/TOF-MS/MS. These differentially expressed proteins act in the nervous transduction, odorant degradation and metabolic change pathways. Further, gene expression patterns in treated and untreated moths were confirmed by qRT-PCR and western blot analysis. RNAi of the chemosensory protein (PxCSP) gene resulted in oviposition significantly increased on cabbage plants treated with rhodojaponin III.
  
  
  CONCLUSIONS
  These rhodojaponin III-induced proteins and gene properties analysis would be essential for a better understanding of the potential molecular mechanism of the response to rhodojaponin III from moths of P. xylostella.
authors.affiliation: Laboratory of Insect Toxicology, College of Resources and Environment, South China Agricultural University, Guangzhou, China.
authors.forename: Xiaolin
authors.initials: X
authors.lastname: Dong
authors.forename: Yifan
authors.initials: Y
authors.lastname: Zhai
authors.forename: Meiying
authors.initials: M
authors.lastname: Hu
authors.forename: Guohua
authors.initials: G
authors.lastname: Zhong
authors.forename: Wanjun
authors.initials: W
authors.lastname: Huang
authors.forename: Zhihua
authors.initials: Z
authors.lastname: Zheng
authors.forename: Pengfei
authors.initials: P
authors.lastname: Han
mesh_terms: D000818:Animals; D004224:Diterpenes / Q000494:pharmacology*; D005260:Female; D005786:Gene Expression Regulation; D055785:Gene Knockdown Techniques; D019476:Insect Proteins / Q000235:genetics / Q000378:metabolism; D007306:Insecticides / Q000494:pharmacology*; D008297:Male; D009036:Moths / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism*; D010058:Oviposition / Q000235:genetics; D020543:Proteome; D040901:Proteomics / Q000379:methods; D034622:RNA Interference; D015203:Reproducibility of Results; D059467:Transcriptome
chemical_list: D004224:Diterpenes; D019476:Insect Proteins; D007306:Insecticides; D020543:Proteome; C069461:rhodojaponin III