title: Cutaneous lesions associated with dual infection caused by canine distemper virus and orthopoxvirus in a domestic cat.
abstract: BACKGROUND
  Within the context of an increased epidemiological pressure caused by canine distemper virus (CDV) in Switzerland together with a potential re-emergence of endemic pathogens such as orthopoxviruses (OPXV), dual infections are possible among susceptible species.
  
  
  OBJECTIVE
  To describe a case of concurrent CDV and OPXV infection in a cat.
  
  
  METHODS
  A 5-year-old, neutered male cat was presented with erythema, crusts and ulcerations around the left eye. High-grade pruritus and a severe conjunctivitis were also present.
  
  
  METHODS
  Formalin-fixed skin biopsy samples were obtained from lesional skin. Histopathology, CDV immunohistochemistry and CDV and OPXV RT-PCR were performed.
  
  
  RESULTS
  Histopathological examination showed severe epidermal necrosis extending to the follicular walls and a dermal infiltration, predominantly eosinophilic. Intranuclear and intracytoplasmic eosinophilic inclusion bodies were visible in the wall of affected hair follicles, with occasional formation of syncytia. The RT-PCR revealed the contextual presence of both CDV and OPXV. Scattered cells stained positive for CDV by immunohistochemistry.
  
  
  CONCLUSIONS
  Dual infections with CDV and OPXV, although rare, may occur and represent additional differential diagnoses for ulcerative skin lesions in cats.
authors.affiliation: Institute of Animal Pathology, Vetsuisse Faculty, University of Bern, Länggasstrasse 122, PO Box 8466, 3001 Bern, Switzerland; DermFocus, Vetsuisse Faculty, University of Bern, Länggasstrasse 122, PO Box 8466, 3001 Bern, Switzerland.
authors.forename: Dominique J
authors.initials: DJ
authors.lastname: Wiener
authors.forename: Monika M
authors.initials: MM
authors.lastname: Welle
authors.forename: Francesco C
authors.initials: FC
authors.lastname: Origgi
mesh_terms: D000818:Animals; D002371:Cat Diseases / Q000473:pathology / Q000821:virology*; D002415:Cats; D004216:Distemper / Q000473:pathology* / Q000821:virology; D004217:Distemper Virus, Canine / Q000302:isolation & purification*; D005128:Eye Diseases / Q000662:veterinary / Q000821:virology; D008297:Male; D018155:Orthopoxvirus / Q000302:isolation & purification*; D011213:Poxviridae Infections / Q000473:pathology / Q000662:veterinary*; D017193:Skin Diseases, Viral / Q000473:pathology*