title: Intralesional injection of rose bengal induces a systemic tumor-specific immune response in murine models of melanoma and breast cancer.
abstract: Intralesional (IL) injection of PV-10 has shown to induce regression of both injected and non-injected lesions in patients with melanoma. To determine an underlying immune mechanism, the murine B16 melanoma model and the MT-901 breast cancer model were utilized. In BALB/c mice bearing MT-901 breast cancer, injection of PV-10 led to regression of injected and untreated contralateral subcutaneous lesions. In a murine model of melanoma, B16 cells were injected into C57BL/6 mice to establish one subcutaneous tumor and multiple lung lesions. Treatment of the subcutaneous lesion with a single injection of IL PV-10 led to regression of the injected lesion as well as the distant B16 melanoma lung metastases. Anti-tumor immune responses were measured in splenocytes collected from mice treated with IL PBS or PV-10. Splenocytes isolated from tumor bearing mice treated with IL PV-10 demonstrated enhanced tumor-specific IFN-gamma production compared to splenocytes from PBS-treated mice in both models. In addition, a significant increase in lysis of B16 cells by T cells isolated after PV-10 treatment was observed. Transfer of T cells isolated from tumor-bearing mice treated with IL PV-10 led to tumor regression in mice bearing B16 melanoma. These studies establish that IL PV-10 therapy induces tumor-specific T cell-mediated immunity in multiple histologic subtypes and support the concept of combining IL PV10 with immunotherapy for advanced malignancies.
authors.affiliation: H. Lee Moffitt Cancer Center and Research Institute, Immunology Program, Tampa, Florida, USA.
authors.forename: Paul
authors.initials: P
authors.lastname: Toomey
authors.forename: Krithika
authors.initials: K
authors.lastname: Kodumudi
authors.forename: Amy
authors.initials: A
authors.lastname: Weber
authors.forename: Lisa
authors.initials: L
authors.lastname: Kuhn
authors.forename: Ellen
authors.initials: E
authors.lastname: Moore
authors.forename: Amod A
authors.initials: AA
authors.lastname: Sarnaik
authors.forename: Shari
authors.initials: S
authors.lastname: Pilon-Thomas
mesh_terms: D019264:Adoptive Transfer; D000818:Animals; D001943:Breast Neoplasms / Q000276:immunology*; D045744:Cell Line, Tumor; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D015552:Injections, Intralesional; D008545:Melanoma / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D012395:Rose Bengal / Q000008:administration & dosage*
chemical_list: D012395:Rose Bengal