title: Distinct metabolites for photoreactive L-phenylalanine derivatives in Klebsiella sp. CK6 isolated from rhizosphere of a wild dipterocarp sapling.
abstract: Photoaffinity labeling is a reliable analytical method for biological functional analysis. Three major photophores--aryl azide, benzophenone and trifluoromethyldiazirine--are utilized in analysis. Photophore-bearing L-phenylalanine derivatives, which are used for biological functional analysis, were inoculated into a Klebsiella sp. isolated from the rhizosphere of a wild dipterocarp sapling in Central Kalimantan, Indonesia, under nitrogen-limiting conditions. The proportions of metabolites were quite distinct for each photophore. These results indicated that photophores affected substrate recognition in rhizobacterial metabolic pathways, and differential photoaffinity labeling could be achieved using different photophore-containing L-phenylalanine derivatives.
authors.affiliation: Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University; Kita 9, Nishi 9, Kita-ku, Sapporo 060-8589, Japan.
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.forename: Wataru
authors.initials: W
authors.lastname: Hisano
authors.forename: Yuta
authors.initials: Y
authors.lastname: Murai
authors.forename: Munenori
authors.initials: M
authors.lastname: Sakurai
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Muto
authors.forename: Haruka
authors.initials: H
authors.lastname: Ikemoto
authors.forename: Masashi
authors.initials: M
authors.lastname: Okamoto
authors.forename: Takashi
authors.initials: T
authors.lastname: Murotani
authors.forename: Reika
authors.initials: R
authors.lastname: Isoda
authors.forename: Dongyeop
authors.initials: D
authors.lastname: Kim
authors.forename: Yasuko
authors.initials: Y
authors.lastname: Sakihama
authors.forename: Irnayuli R
authors.initials: IR
authors.lastname: Sitepu
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Hashidoko
authors.forename: Yasumaru
authors.initials: Y
authors.lastname: Hatanaka
authors.forename: Makoto
authors.initials: M
authors.lastname: Hashimoto
mesh_terms: D001577:Benzophenones / Q000737:chemistry; D053958:Dipterocarpaceae / Q000382:microbiology; D007709:Klebsiella / Q000145:classification / Q000302:isolation & purification / Q000378:metabolism*; D010649:Phenylalanine / Q000031:analogs & derivatives / Q000737:chemistry / Q000378:metabolism*; D019920:Photoaffinity Labels; D058441:Rhizosphere; D014364:Tryptophan / Q000737:chemistry / Q000378:metabolism*
chemical_list: D001577:Benzophenones; D019920:Photoaffinity Labels; D010649:Phenylalanine; C047723:benzophenone; D014364:Tryptophan