title: Salivary biomarkers: relationship between oxidative stress and alveolar bone loss in chronic periodontitis.
abstract: OBJECTIVE
  Oxidative stress is implicated in the pathogenesis of many systemic and oral diseases such as periodontal disease. The main aim of this study is to explore a possible association between salivary markers of OS and alveolar bone loss.
  
  
  METHODS
  The study included 20 patients with chronic periodontitis and 20 controls. Salivary OS biomarkers 8-hidroxy-desoxguanosine (8-HOdG), malondialdehyde (MDA), uric acid, total antioxidant capacity (TAC) and glutathione peroxidase (GPx) were evaluated. Bone loss markers such as C-terminal telopeptide of type I collagen (CTX I), matrix metalloproteinases-8 (MMP-8), osteocalcin and 25-hydroxy vitamin D3 (25- OH D) were detected in this study. The methods included general biochemical tests and ELISA.
  
  
  RESULTS
  Salivary 8-OHdG, MDA levels were significantly higher in the chronic periodontitis group compared with controls (p < 0.05). Salivary activities for uric acid, TAC and GPx were significantly decreased in patients with chronic periodontitis vs controls (p < 0.05). Salivary levels for CTX I, MMP-8, 25-OH D and Osteocalcin were significantly higher in the chronic periodontitis group compared to the controls (p < 0.05). A significant positive correlation was observed between salivary levels of MDA and CTX I. Significant negative correlations between uric acid and CTX I and between MMP-8 and uric acid have been found. Significant positive correlations were observed between CTX I, MMP-8, 25-OH D, osteocalcin and clinical parameters of periodontal disease.
  
  
  CONCLUSIONS
  Important oxidative stress associated with alveolar bone loss biomarkers can be detected in saliva of patients with periodontal disease.
authors.affiliation: Department of Biochemistry.
authors.forename: Daniela
authors.initials: D
authors.lastname: Miricescu
authors.forename: Alexandra
authors.initials: A
authors.lastname: Totan
authors.forename: Bogdan
authors.initials: B
authors.lastname: Calenic
authors.forename: Brandusa
authors.initials: B
authors.lastname: Mocanu
authors.forename: Andreea
authors.initials: A
authors.lastname: Didilescu
authors.forename: Maria
authors.initials: M
authors.lastname: Mohora
authors.forename: Tudor
authors.initials: T
authors.lastname: Spinu
authors.forename: Maria
authors.initials: M
authors.lastname: Greabu
mesh_terms: D000328:Adult; D016301:Alveolar Bone Loss / Q000378:metabolism*; D015415:Biomarkers / Q000378:metabolism*; D002908:Chronic Disease; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress; D010518:Periodontitis / Q000378:metabolism* / Q000473:pathology; D012463:Saliva / Q000378:metabolism*
chemical_list: D015415:Biomarkers