title: [Transcript profile of converting xylose and glucose to ethanol by Candida shehatae].
abstract: OBJECTIVE
  We detected and analyzed transcript profile differences between hexose and pentose fermentation by Candida shehatae, a typical xylose fermenting yeast strain. On this basis, the encoding genes of key enzymes and functional protein were screened for discovering candidates of metabolism and regulation.
  
  
  METHODS
  To discover the key genes of xylose metabolism and ethanol fermentation in Candida shehatae, we performed a new high throughout de novo transcriptome sequencing technology on Roche 454 GS FLX Titanium platform. Two cDNA libraries were constructed and sequenced for xylose and glucose fermentation for comparison of its expressed sequence tags differences.
  
  
  RESULTS
  Second sequencing run resulted in 600000 reads with the average length of 400bp for each cDNA library. We got 7250 and 7168 contigs by assembly, and annotated 2412 and 2456 unique genes by BLAST and Gene Ontology analysis for xylose and glucose respectively. By comparison, we identified 158 genes as different expression genes candidates at p < 0.05 for xylose metabolism and ethanol fermentation in Candida shehatae.
  
  
  CONCLUSIONS
  The group genes for xylose metabolism and ethanol fermentation in Candida shehatae was discovered by using transcript profile sequencing and comparison. It will provide fundamental data for the relative research on molecular biology and metabolic regulation.
authors.affiliation: College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, China. littlexiongbaby@163.com
authors.forename: Xujie
authors.initials: X
authors.lastname: Xiong
authors.forename: Peng
authors.initials: P
authors.lastname: Cai
authors.forename: Yong
authors.initials: Y
authors.lastname: Xu
authors.forename: Qiang
authors.initials: Q
authors.lastname: Yong
authors.forename: Shiyuan
authors.initials: S
authors.lastname: Yu
mesh_terms: D002175:Candida / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism*; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation / Q000235:genetics; D020869:Gene Expression Profiling / Q000379:methods; D005947:Glucose / Q000235:genetics* / Q000378:metabolism*; D006601:Hexoses / Q000235:genetics / Q000378:metabolism; D059467:Transcriptome; D014994:Xylose / Q000235:genetics* / Q000378:metabolism*
chemical_list: D006601:Hexoses; D000431:Ethanol; D014994:Xylose; D005947:Glucose