title: [Effects of antagonist and agonist of nicotinic acetylcholine receptors on injury of rat neurons induced by amyloid β-protein].
abstract: OBJECTIVE
  To explore the chronic effects of nicotinic antagonist and agonist on rat neurons injury induced by β-amyloid protein.
  
  
  METHODS
  The rat model of neuron injury was established by the exposure to Aβ25-35 and the intervention agent was either methyllycaconitine (MLA) or nicotine (Nic). And the experimental groups were control (distilled water), Aβ25-35, MLA (MLA and Aβ25-35) and Nic (Nic and Aβ25-35). Cellular viability was detected by methyl thiazolyl tetrazolium (MTT) chromatometry while apoptosis and necrosis were detected by flow cytometer.
  
  
  RESULTS
  Compared with control, cellular viability decreased while the apoptotic and necrotic rates increased in Aβ25-35 group(P = 0.00). The values of cellular viability at (0.75 ± 0.02) and (0.75 ± 0.09) in Aβ25-35 and MLA groups respectively were significantly lower than that of Nic group (0.81 ± 0.02, P = 0.01) at Day 3 and 7. No significant differences existed in cellular viability between Aβ25-35 and MLA groups. At Day 14, the differences of cellular viability were not obvious in all groups. At Day 21, cell viability of MLA group (0.64 ± 0.10) was significantly higher than those of Aβ25-35 (0.57 ± 0.04, P = 0.019) and Nic groups (0.56 ± 0.04, P = 0.008). The apoptotic rate was lower than that of Aβ25-35 group (3.70% ± 0.20% vs 4.70% ± 0.46%, P = 0.008) while the necrotic rate lower than that of Aβ25-35 group (7.73% ± 0.86% vs 16.30% ± 1.05%, P = 0.00) and Nic group (16.03% ± 1.53%, P = 0.00). However, no significant differences existed in cellular viability or apoptotic and necrotic rate between Aβ25-35 and Nic groups.
  
  
  CONCLUSIONS
  With chronic treatment, the protective effect of α7 nicotinic antagonist methyllycaconitine increases whereas that of nicotinic agonist nicotine decreases.
authors.affiliation: Department of Critical Care Medicine, First Affiliated Hospital, Jinan University, Guangzhou 510630, China.
authors.forename: Zhi-gang
authors.initials: ZG
authors.lastname: Wang
authors.forename: Ren-bin
authors.initials: RB
authors.lastname: Qi
authors.forename: Li-hong
authors.initials: LH
authors.lastname: Zhu
authors.forename: Da-xiang
authors.initials: DX
authors.lastname: Lu
mesh_terms: D016229:Amyloid beta-Peptides / Q000633:toxicity*; D000818:Animals; D000831:Animals, Newborn; D017209:Apoptosis / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism; D018722:Nicotinic Agonists / Q000494:pharmacology*; D018733:Nicotinic Antagonists / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D011978:Receptors, Nicotinic
chemical_list: D016229:Amyloid beta-Peptides; D018722:Nicotinic Agonists; D018733:Nicotinic Antagonists; D011978:Receptors, Nicotinic