title: Structure and gene cluster of the O-antigen of Escherichia coli O154.
abstract: The O-polysaccharide (O-antigen) of Escherichia coli O154 was studied by sugar analysis along with 1D and 2D (1)H and (13)C NMR spectroscopy. The following structure of the branched pentasaccharide repeating unit was established: [structure: see text]. The O-antigen gene cluster of E. coli O154 was sequenced. The gene functions were tentatively assigned by comparison with sequences in the available databases and found to be in full agreement with the O-polysaccharide structure.
authors.affiliation: ND Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russian Federation. perepel@ioc.ac.ru
authors.forename: Andrei V
authors.initials: AV
authors.lastname: Perepelov
authors.forename: Quan
authors.initials: Q
authors.lastname: Wang
authors.forename: Sof'ya N
authors.initials: SN
authors.lastname: Senchenkova
authors.forename: Lu
authors.initials: L
authors.lastname: Feng
authors.forename: Alexander S
authors.initials: AS
authors.lastname: Shashkov
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.forename: Yuriy A
authors.initials: YA
authors.lastname: Knirel
mesh_terms: D002236:Carbohydrate Conformation; D004926:Escherichia coli / Q000737:chemistry* / Q000235:genetics*; D009682:Magnetic Resonance Spectroscopy; D019081:O Antigens / Q000737:chemistry* / Q000235:genetics* / Q000302:isolation & purification; D012015:Reference Standards; D017422:Sequence Analysis, DNA
chemical_list: D019081:O Antigens
keywords: Bacterial polysaccharide structure; Escherichia coli; Lipopolysaccharide; O-antigen; O-antigen gene cluster