title: Germes is involved in translocation of germ plasm during development of Xenopus primordial germ cells.
abstract: Germes mRNA and protein are components of the germ plasm in Xenopus laevis. Previously, based on phenotypic observations of tailbud embryos expressing intact and mutant Germes, it was suggested that Germes is involved in the organization of germ plasm (Berekelya et al., 2007). Recently, to observe the germ plasm in a living embryo, we generated transgenic Xenopus expressing EGFP fused with a mitochondrial targeting signal, because germ plasm is enriched with mitochondria (Taguchi et al., 2012). Using this transgenic Xenopus, we demonstrate that Germes plays an essential role in the translocation of germ plasm from the cortex to the perinuclear region in primordial germ cells during early gastrulation.
authors.affiliation: Department of Life Science, University of Hyogo, Kamigori, Akou-gun, Hyogo, Japan. ytakeshi@nibb.ac.jp
authors.forename: Takeshi
authors.initials: T
authors.lastname: Yamaguchi
authors.forename: Ayaka
authors.initials: A
authors.lastname: Taguchi
authors.forename: Kenji
authors.initials: K
authors.lastname: Watanabe
authors.forename: Hidefumi
authors.initials: H
authors.lastname: Orii
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D001692:Biological Transport; D036703:Blastula / Q000166:cytology / Q000196:embryology / Q000378:metabolism; D002467:Cell Nucleus / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism*; D004625:Embryo, Nonmammalian / Q000166:cytology / Q000196:embryology / Q000378:metabolism; D005260:Female; D054262:Gastrulation / Q000235:genetics; D018507:Gene Expression Regulation, Developmental; D005854:Germ Cells / Q000166:cytology / Q000378:metabolism*; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D008845:Microinjections; D008870:Microtubules / Q000378:metabolism; D008928:Mitochondria / Q000235:genetics / Q000378:metabolism; D008938:Mitosis / Q000235:genetics; D009154:Mutation; D009865:Oocytes / Q000166:cytology / Q000378:metabolism; D012333:RNA, Messenger / Q000008:administration & dosage / Q000235:genetics / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D013997:Time Factors; D029867:Xenopus Proteins / Q000235:genetics* / Q000378:metabolism; D014982:Xenopus laevis / Q000196:embryology / Q000235:genetics* / Q000378:metabolism
chemical_list: C525160:Germes protein, Xenopus; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D029867:Xenopus Proteins; D049452:Green Fluorescent Proteins