title: High throughput sequencing of the Angiostrongylus cantonensis genome: a parasite spreading worldwide.
abstract: Angiostrongylus cantonensis is a parasitic nematode of rodents and a leading aetiological agent of eosinophilic meningitis in humans. Definitive diagnosis is difficult, often relying on immunodiagnostic methods which utilize crude antigens. New immunodiagnostic methods based on recombinant proteins are being developed, and ideally these methods would be made available worldwide. Identification of diagnostic targets, as well as studies on the biology of the parasite, are limited by a lack of molecular information on Angiostrongylus spp. available in databases. In this study we present data collected from DNA random high-throughput sequencing together with proteomic analyses and a cDNA walking methodology to identify and obtain the nucleotide or amino acid sequences of unknown immunoreactive proteins. 28 080 putative ORFs were obtained, of which 3371 had homology to other deposited protein sequences. Using the A. cantonensis genomic sequences, 156 putative ORFs, matching peptide sequences obtained from previous proteomic studies, were considered novel, with no homology to existing sequences. Full-length coding sequences of eight antigenic target proteins were obtained. In this study we generated not only the complete nucleotide sequences of the antigenic protein targets but also a large amount of genomic data which may help facilitate future genomic, proteomic, transcriptomic or metabolomic studies on Angiostrongylus.
authors.affiliation: Laboratório de Biologia Parasitária da Faculdade de Biociências e Laboratório de Parasitologia Molecular do Instituto de Pesquisas Biomédicas da Pontifícia Universidade do Rio Grande do Sul (PUCRS), Av Ipiranga 6690, 90690-900 Porto Alegre RS, Brasil.
authors.forename: Alessandra L
authors.initials: AL
authors.lastname: Morassutti
authors.affiliation: Division of Parasitic Diseases & Malaria, Center for Global Health, Centers for Disease Control and Prevention, 1600 Clifton Road NE, Atlanta, GA, 30329, USA.
authors.forename: Andrey
authors.initials: A
authors.lastname: Perelygin
authors.affiliation: Programa de Pós-Graduação em Genética e Biologia Molecular, Universidade Federal do Rio Grande do Sul, Porto Alegre RS, Brasil.
authors.forename: Marcos O
authors.initials: MO
authors.lastname: DE Carvalho
authors.affiliation: Universidade Federal do Pampa - Campus São Gabriel, Av. Antônio Trilha, 1847 - São Gabriel - RS - CEP: 97300-000.
authors.forename: Leandro Nascimento
authors.initials: LN
authors.lastname: Lemos
authors.affiliation: Universidade Federal do Pampa - Campus São Gabriel, Av. Antônio Trilha, 1847 - São Gabriel - RS - CEP: 97300-000.
authors.forename: Paulo Marcos
authors.initials: PM
authors.lastname: Pinto
authors.affiliation: Division of Parasitic Diseases & Malaria, Center for Global Health, Centers for Disease Control and Prevention, 1600 Clifton Road NE, Atlanta, GA, 30329, USA.
authors.forename: Mike
authors.initials: M
authors.lastname: Frace
authors.affiliation: Division of Parasitic Diseases & Malaria, Center for Global Health, Centers for Disease Control and Prevention, 1600 Clifton Road NE, Atlanta, GA, 30329, USA.
authors.forename: Patricia P
authors.initials: PP
authors.lastname: Wilkins
authors.affiliation: Laboratório de Biologia Parasitária da Faculdade de Biociências e Laboratório de Parasitologia Molecular do Instituto de Pesquisas Biomédicas da Pontifícia Universidade do Rio Grande do Sul (PUCRS), Av Ipiranga 6690, 90690-900 Porto Alegre RS, Brasil.
authors.forename: Carlos
authors.initials: C
authors.lastname: Graeff-Teixeira
authors.affiliation: Division of Parasitic Diseases & Malaria, Center for Global Health, Centers for Disease Control and Prevention, 1600 Clifton Road NE, Atlanta, GA, 30329, USA.
authors.forename: Alexandre J
authors.initials: AJ
authors.lastname: DA Silva
mesh_terms: D017185:Angiostrongylus cantonensis / Q000235:genetics* / Q000276:immunology; D000818:Animals; D049751:Genome, Helminth / Q000235:genetics*; D015801:Helminth Proteins / Q000235:genetics; D059014:High-Throughput Nucleotide Sequencing; D040901:Proteomics; D017206:Strongylida Infections / Q000276:immunology / Q000469:parasitology*
chemical_list: D015801:Helminth Proteins