title: A monosaccharide-modified peptide phage library for screening of ligands to carbohydrate-binding proteins.
abstract: A monosaccharide-modified β-loop peptide library displayed on phage has been constructed and used for the screening of glycopeptide ligands against a carbohydrate-binding protein. The β-loop peptide library was designed and modified with a mannose derivative on phage. The glycopeptide ligands to concanavalin A (ConA), a mannose-binding protein, were obtained from the mannose-modified peptide phage library. The amino acids neighboring the mannose unit of glycopeptides not only reinforced the binding affinity but also gave diverse binding characteristics.
authors.affiliation: Department of Bioengineering, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Nagatsuta-cho 4259 B-40, Midori-ku, Yokohama 226-8501, Japan.
authors.forename: Kanako
authors.initials: K
authors.lastname: Arai
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Tsutsumi
authors.forename: Hisakazu
authors.initials: H
authors.lastname: Mihara
mesh_terms: D000595:Amino Acid Sequence; D003208:Concanavalin A / Q000378:metabolism*; D006020:Glycopeptides / Q000737:chemistry / Q000378:metabolism*; D008358:Mannose / Q000737:chemistry / Q000378:metabolism*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D019151:Peptide Library
chemical_list: D006020:Glycopeptides; D019151:Peptide Library; D003208:Concanavalin A; D008358:Mannose
keywords: Carbohydrate-binding protein; Modification; Peptide; Phage display; Saccharide