title: The stimulatory effect of TLRs ligands on maturation of chicken bone marrow-derived dendritic cells.
abstract: Dendritic cells (DCs) are crucial for initiation of both innate and adaptive immune responses. TLR ligands combine with Toll-like receptors (TLRs) expressed on the DC surface and induce DC maturation. The potential effect of three types of TLR ligands (Bacillus subtilis (B. subtilis) spores, polyinosinic-polycytidylic acid and CpG oligodeoxynucleotides) on chicken bone marrow-derived DCs (chBM-DCs) maturation was studied. The chBM-DCs cultured in presence of recombinant chicken granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin (IL)-4 displayed the typical morphology of DCs after 7 days of culture. These immature chBM-DCs up-regulated the expression of MHC-II and of the putative CD11c, but had yet low to moderate levels of the CD40 and CD86 co-stimulatory molecules. After stimulation by the TLR ligands, the chBM-DCs displayed a more mature morphologic phenotype, significantly increased the CD40 and CD86 cell surface expression levels and gained the ability to stimulate proliferation of naive T cells in the allogeneic mixed lymphocyte reaction, compared to the immature chBM-DCs. In conclusion, our data demonstrated that all three TLR ligands were strong stimuli for driving chBM-DCs maturation in vitro, with B. subtilis spores being the most efficient.
authors.affiliation: Key Lab of Animal Physiology and Biochemistry, Ministry of Agriculture, Nanjing Agricultural University, Weigang 1, Nanjing, Jiangsu 210095, China.
authors.forename: Jinfeng
authors.initials: J
authors.lastname: Liang
authors.forename: Jia
authors.initials: J
authors.lastname: Fu
authors.forename: Haihong
authors.initials: H
authors.lastname: Kang
authors.forename: Jian
authors.initials: J
authors.lastname: Lin
authors.forename: Qinghua
authors.initials: Q
authors.lastname: Yu
authors.forename: Qian
authors.initials: Q
authors.lastname: Yang
mesh_terms: D000818:Animals; D001412:Bacillus subtilis / Q000502:physiology; D001854:Bone Marrow Cells / Q000502:physiology*; D002645:Chickens / Q000276:immunology*; D003713:Dendritic Cells / Q000502:physiology*; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D015847:Interleukin-4 / Q000494:pharmacology; D008024:Ligands; D008213:Lymphocyte Activation; D009838:Oligodeoxyribonucleotides / Q000494:pharmacology; D011070:Poly I-C / Q000494:pharmacology; D013154:Spleen / Q000166:cytology; D013171:Spores, Bacterial / Q000502:physiology; D051193:Toll-Like Receptors / Q000502:physiology*
chemical_list: C408982:CPG-oligonucleotide; D008024:Ligands; D009838:Oligodeoxyribonucleotides; D051193:Toll-Like Receptors; D015847:Interleukin-4; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D011070:Poly I-C
keywords: Bone marrow; Chicken; Dendritic cells; TLR ligands