title: Highly pathogenic avian influenza A(H5N1) mutants transmissible by air are susceptible to human and animal neutralizing antibodies.
abstract: A laboratory-generated reassortant H5 hemagglutinin (HA)/influenza A(H1N1) strain containing 4 mutations in influenza A(H5N1) HA has become transmissible by air among mammals. Here, we constructed 15 influenza A(H5N1) pseudoviruses containing a single mutation or a combination of mutations and showed that the pseudoviruses were susceptible to neutralizing antibodies from patients with influenza A(H5N1) infection and from mice immunized with a vaccine containing the conserved HA1 sequence of influenza A(H5N1). These results indicate that antibodies in patients currently infected by influenza A(H5N1) and antibodies induced by vaccines containing conserved sequences in HA1 of wild-type influenza A(H5N1) are highly effective in cross-neutralizing future influenza A(H5N1) mutants with airborne transmissibility, suggesting that human influenza pandemics caused by these influenza A(H5N1) variants can be prevented.
authors.affiliation: Lindsley F. Kimball Research Institute, New York Blood Center, New York City.
authors.forename: Lanying
authors.initials: L
authors.lastname: Du
authors.forename: Ye
authors.initials: Y
authors.lastname: Li
authors.forename: Guangyu
authors.initials: G
authors.lastname: Zhao
authors.forename: Lili
authors.initials: L
authors.lastname: Wang
authors.forename: Peng
authors.initials: P
authors.lastname: Zou
authors.forename: Lu
authors.initials: L
authors.lastname: Lu
authors.forename: Yusen
authors.initials: Y
authors.lastname: Zhou
authors.forename: Shibo
authors.initials: S
authors.lastname: Jiang
mesh_terms: D000818:Animals; D057134:Antibodies, Neutralizing / Q000097:blood / Q000276:immunology* / Q000494:pharmacology*; D000914:Antibodies, Viral / Q000097:blood / Q000276:immunology*; D006801:Humans; D053124:Influenza A Virus, H5N1 Subtype / Q000235:genetics / Q000276:immunology* / Q000472:pathogenicity; D007252:Influenza Vaccines / Q000276:immunology / Q000494:pharmacology; D007251:Influenza, Human / Q000276:immunology* / Q000517:prevention & control / Q000635:transmission / Q000821:virology*; D051379:Mice; D009500:Neutralization Tests
chemical_list: D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D007252:Influenza Vaccines
keywords: Air-transmissibility; H5N1 influenza virus; Neutralizing antibodies; Patient serum specimens