title: Statistical approach for the enhanced production of cold-active β-galactosidase from Thalassospira frigidphilosprofundus: a novel marine psychrophile from deep waters of Bay of Bengal.
abstract: In the present investigation Thalassospira frigidphilosprofundus, a novel species from the deep waters of the Bay of Bengal, was explored for the production of cold-active β-galactosidase by submerged fermentation using marine broth medium as the basal medium. Effects of various medium constituents, namely, carbon, nitrogen source, pH, and temperature, were investigated using a conventional one-factor-at-a-time method. It was found that lactose, yeast extract, and bactopeptones are the most influential components for β-galactosidase production. Under optimal conditions, the production of β-galactosidase was found to be 3,864 U/mL at 20 ± 2°C, pH 6.5 ± 0.2, after 48 hr of incubation. β-Galactosidase production was further optimized by the Taguchi orthogonal array design of experiments and the central composite rotatable design (CCRD) of response surface methodology. Under optimal experimental conditions the cold-active β-galactosidase enzyme production from Thalassospira frigidphilosprofundus was enhanced from 3,864 U/mL to 10,657 U/mL, which is almost three times higher than the cold-active β-galactosidase production from the well-reported psychrophile Pseudoalteromonas haloplanktis.
authors.affiliation: Center for Bioseparation Technology, VIT University, Vellore, India. pkkanth2006@gmail.com
authors.forename: K K
authors.initials: KK
authors.lastname: Pulicherla
authors.forename: P Suresh
authors.initials: PS
authors.lastname: Kumar
authors.forename: K
authors.initials: K
authors.lastname: Manideep
authors.forename: V P B
authors.initials: VP
authors.lastname: Rekha
authors.forename: Mrinmoy
authors.initials: M
authors.lastname: Ghosh
authors.forename: K R S
authors.initials: KR
authors.lastname: Sambasiva Rao
mesh_terms: D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000302:isolation & purification; D061006:Bays / Q000382:microbiology*; D002244:Carbon / Q000378:metabolism; D003080:Cold Temperature; D003470:Culture Media / Q000737:chemistry; D005285:Fermentation; D006863:Hydrogen-Ion Concentration; D007194:India; D007700:Kinetics; D007785:Lactose / Q000378:metabolism; D009584:Nitrogen / Q000378:metabolism; D025341:Principal Component Analysis; D012244:Rhodospirillaceae / Q000737:chemistry* / Q000201:enzymology; D001616:beta-Galactosidase / Q000096:biosynthesis* / Q000302:isolation & purification
chemical_list: D001426:Bacterial Proteins; D003470:Culture Media; D002244:Carbon; D001616:beta-Galactosidase; D007785:Lactose; D009584:Nitrogen