title: Cell pairing using a dielectrophoresis-based device with interdigitated array electrodes.
abstract: We present a chip device with an array of 900 gourd-shaped microwells designed to pair single cells of different types. The device consists of interdigitated array (IDA) electrodes and uses positive dielectrophoresis to trap cells within the microwells. Each side of a microwell is on a different comb of the IDA, so that cells of different types are trapped on opposite sides of the microwells, leading to close cell pairing. Using this device, a large number of cell pairs can be formed easily and rapidly, making it a highly attractive tool for controllable cell pairing in a range of biological applications.
authors.affiliation: Graduate School of Environmental Studies, Tohoku University, Sendai 980-8579, Japan.
authors.forename: Mustafa
authors.initials: M
authors.lastname: Şen
authors.forename: Kosuke
authors.initials: K
authors.lastname: Ino
authors.forename: Javier
authors.initials: J
authors.lastname: Ramón-Azcón
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Shiku
authors.forename: Tomokazu
authors.initials: T
authors.lastname: Matsue
mesh_terms: D016475:3T3 Cells; D000818:Animals; D004566:Electrodes; D004586:Electrophoresis / Q000295:instrumentation*; D053595:Embryonic Stem Cells / Q000166:cytology; D051379:Mice; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation; D008856:Microscopy, Fluorescence; D046888:Tissue Array Analysis / Q000295:instrumentation*