title: Expression of p27Kip1 protein in gastric carcinoma.
abstract: OBJECTIVE
  p27 protein resulted in the accumulation of cyclin E/cyclin dependent kinase 2/p27 ternary complexes inhibits gap1 to synthesis phase transition. Here, we have investigated the correlations, if any, between the expressions of p27 and p53, and proliferation cell nuclear antigen.
  
  
  METHODS
  A retrospective study was performed on 75 cases of gastric cancer that had undergone surgical resection. Immunohistochemical staining was performed using the avidin-biotin-peroxidase complex technique method, with anti-p27 antibody, anti-p53 antibody and anti-proliferation cell nuclear antigen antibody.
  
  
  RESULTS
  The rate of lymph node metastasis in the p27 negative cases was significantly higher than that in the p27 positive cases.The rate of tumor limited to the gastric wall in the p27 positive cases was significantly higher than-that in the p27 negative cases.The mean proliferation cell nuclear antigen index of the p27 negative cases was significantly higher than that of the p27 positive cases. The survival rate of the p27 positive cases was significantly higher than that of the p27 negative cases. In Stage III cases, the survival rate of the p53 negative p27 positive or p53 negative p27 negative cases was significantly higher than that of p53 positive p27 negative cases.
  
  
  CONCLUSIONS
  p27 was correlated with lymph node metastasis, depth of invasion, and proliferative activity of gastric cancer. Immunoreactivity of combination of p53 and p27 was a useful predictive marker of prognosis of gastric cancer.
authors.affiliation: Department of Surgery, Kurume University School of Medicine, Kurume, Japan. keishiro@med.kurume-u.ac.jp
authors.forename: Keishiro
authors.initials: K
authors.lastname: Aoyagi
authors.forename: Kikuo
authors.initials: K
authors.lastname: Kouhuji
authors.forename: Motoshi
authors.initials: M
authors.lastname: Miyagi
authors.forename: Takuya
authors.initials: T
authors.lastname: Imaizumi
authors.forename: Junya
authors.initials: J
authors.lastname: Kizaki
authors.forename: Taro
authors.initials: T
authors.lastname: Isobe
authors.forename: Kazuo
authors.initials: K
authors.lastname: Shirouzu
mesh_terms: D019938:Cyclin D1 / Q000032:analysis; D050760:Cyclin-Dependent Kinase Inhibitor p27 / Q000032:analysis* / Q000502:physiology; D006801:Humans; D007150:Immunohistochemistry; D009367:Neoplasm Staging; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis; D013274:Stomach Neoplasms / Q000737:chemistry* / Q000401:mortality / Q000473:pathology; D016159:Tumor Suppressor Protein p53 / Q000032:analysis
chemical_list: C530104:CCND1 protein, human; C436291:CDKN1B protein, human; D018809:Proliferating Cell Nuclear Antigen; C495901:TP53 protein, human; D016159:Tumor Suppressor Protein p53; D019938:Cyclin D1; D050760:Cyclin-Dependent Kinase Inhibitor p27