title: Strength comparison between cold-inducible promoters of Arabidopsis cor15a and cor15b genes in potato and tobacco.
abstract: The cold-inducible promoter is ideal for regulating ectopic gene expression in plants to cope with the cold stress. The promoters of two cold-regulated genes, cor15a and cor15b, were cloned from Arabidopsis thaliana and their strengths were assayed in potato and tobacco. Although the cis-element composition and cold-inducible property were similar between the two promoters, the cor15b promoter showed significantly higher activity than the cor15a promoter in both potato and tobacco. In order to elucidate the factors determining this discrepancy, cor15a and cor15b promoters were separately truncated from 5'-end to construct short promoters with similar size containing a single C-repeat/dehydration-responsive element (CRT/DRE). Subsequently, two synthetic promoters were constructed by swapping the flanking sequences of CRT/DRE in the truncated promoters. The promoter strength comparison demonstrated that the flanking sequence could affect the promoter strength. These findings provide a potential regulatory mechanism to control the promoter strength without impact on other properties.
authors.affiliation: National Center for Vegetable Improvement (Central China), Key Laboratory of Horticultural Plant Biology (Huazhong Agricultural University), Ministry of Education, Huazhong Agricultural University, Wuhan 430070, People's Republic of China.
authors.forename: Meng
authors.initials: M
authors.lastname: Li
authors.forename: Xiaohuan
authors.initials: X
authors.lastname: Wang
authors.forename: Yang
authors.initials: Y
authors.lastname: Cao
authors.forename: Xun
authors.initials: X
authors.lastname: Liu
authors.forename: Yuan
authors.initials: Y
authors.lastname: Lin
authors.forename: Yongbin
authors.initials: Y
authors.lastname: Ou
authors.forename: Huiling
authors.initials: H
authors.lastname: Zhang
authors.forename: Jun
authors.initials: J
authors.lastname: Liu
mesh_terms: D017360:Arabidopsis / Q000235:genetics*; D003080:Cold Temperature; D018506:Gene Expression Regulation, Plant / Q000235:genetics / Q000502:physiology; D011401:Promoter Regions, Genetic / Q000235:genetics*; D011198:Solanum tuberosum / Q000235:genetics*; D014026:Nicotiana / Q000235:genetics*
keywords: Cold-inducible; Flanking sequence; Potato; Promoter; Strength; Tobacco