title: Norway spruce (Picea abies) genetic transformation with modified Cry3A gene of Bacillus thuringiensis.
abstract: Modified versions of the Cry3A gene of Bacillus thuringiensis (Bt) were transferred into Norway spruce (Picea abies). Both the biolistic approach and Agrobacterium tumefaciens mediated procedure were employed for transformation of embryogenic tissue (ET) cultures. The latter method proved to be more efficient yielding 70 transgenic embryogenic tissue lines compared with 18 lines obtained by biolistics. The modified Cry3A genes were driven by a 35S promoter and the nptII screenable selection marker gene was used in all vectors. The transgenic ETs were molecularly characterized and converted into mature somatic embryos. Germinating embryos formed plantlets which were finally planted into perlite and their Cry3A gene transcription activities were demonstrated by RT-PCR.
authors.affiliation: Institute of Plant Molecular Biology, Biology Centre AS CR, and Faculty of Science, South Bohemia University, České Budějovice, Czech Republic.
authors.forename: Jindřich
authors.initials: J
authors.lastname: Bříza
authors.forename: Daniela
authors.initials: D
authors.lastname: Pavingerová
authors.forename: Josef
authors.initials: J
authors.lastname: Vlasák
authors.forename: Hana
authors.initials: H
authors.lastname: Niedermeierová
mesh_terms: D028202:Abies / Q000196:embryology / Q000235:genetics*; D016960:Agrobacterium tumefaciens / Q000235:genetics*; D001413:Bacillus thuringiensis / Q000235:genetics*; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000235:genetics*; D004731:Endotoxins / Q000235:genetics*; D005822:Genetic Vectors; D006460:Hemolysin Proteins / Q000235:genetics*; D058439:Plant Somatic Embryogenesis Techniques; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic; D012639:Seeds / Q000235:genetics* / Q000254:growth & development; D014158:Transcription, Genetic; D014170:Transformation, Genetic
chemical_list: D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D004731:Endotoxins; D006460:Hemolysin Proteins; C030956:insecticidal crystal protein, Bacillus Thuringiensis