title: Effect of Angelica sinensis Polysaccharides on Osteoarthritis In Vivo and In Vitro: A Possible Mechanism to Promote Proteoglycans Synthesis.
abstract: This study investigated the effect of Angelica sinensis polysaccharides (APS-3c) on rat osteoarthritis (OA) model in vivo and rat interleukin-1-beta- (IL-1 β -) stimulated chondrocytes in vitro. APS-3c was administrated into rat OA knee joints and had protective effects on rat OA cartilage in vivo. Primary rat articular chondrocytes were cotreated with APS-3c and IL-1 β   in vitro. 2~50  μ g/mL APS-3c had no effect on chondrocytes viability, whereas it increased the proteoglycans (PGs) synthesis inhibited by IL-1 β . Microarray analysis showed that the significant changes were concentrated in the genes which were involved in PGs synthesis. RT-PCR confirmed that treatment with APS-3c increased the mRNA expression of aggrecan and glycosyltransferases (GTs) inhibited by IL-1 β but did not affect the mRNA expression of matrix-degrading enzymes. These results indicate that APS-3c can improve PGs synthesis of chondrocytes on rat OA model in vivo and IL-1 β -stimulated chondrocytes in vitro, which is due to the promotion of the expression of aggrecan and GTs involved in PGs synthesis but not the inhibition of the expression of matrix-degrading enzymes. Our findings suggest the clinical relevance of APS-3c in the prospective of future alternative medical treatment for OA.
authors.affiliation: Department of Orthopaedic Surgery, Zhongnan Hospital, Wuhan University, Wuhan, Hubei 430071, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Qin
authors.forename: Yan-Song
authors.initials: YS
authors.lastname: Liu
authors.forename: Jun
authors.initials: J
authors.lastname: Liu
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.forename: Yang
authors.initials: Y
authors.lastname: Tan
authors.forename: Xiao-Jun
authors.initials: XJ
authors.lastname: Li
authors.forename: Jacques
authors.initials: J
authors.lastname: Magdalou
authors.forename: Qi-Bing
authors.initials: QB
authors.lastname: Mei
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
authors.forename: Liao-Bin
authors.initials: LB
authors.lastname: Chen