title: New silica nanostructure for the improved delivery of topical antibiotics used in the treatment of staphylococcal cutaneous infections.
abstract: In this paper, we report the synthesis, characterization (FT-IR, XRD, BET, HR-TEM) and bioevaluation of a novel γ-aminobutiric acid/silica (noted GABA-SiO₂ or γ-SiO₂) hybrid nanostructure, for the improved release of topical antibiotics, used in the treatment of Staphylococcus aureus infections. GABA-SiO₂ showed IR bands which were assigned to Si-O-Si (stretch mode). The XRD pattern showed a broad peak in the range of 18-30° (2θ), indicating an amorphous structure. Based on the BET analysis, estimations about surface area (438.14 m²/g) and pore diameters (4.76 nm) were done. TEM observation reveals that the prepared structure presented homogeneity and an average size of particles not exceeding 10nm. The prepared nanostructure has significantly improved the anti-staphylococcal activity of bacitracin and kanamycin sulfate, as demonstrated by the drastic decrease of the minimal inhibitory concentration of the respective antibiotics loaded in the GABA-SiO₂ nanostructure. These results, correlated with the high biocompatibility of this porous structure, are highlighting the possibility of using this carrier for the local delivery of the antimicrobial substances in lower active doses, thus reducing their cytotoxicity and side-effects.
authors.affiliation: Politehnica University of Bucharest, Faculty of Applied Chemistry and Materials Science, Department of Science and Engineering of Oxide Materials and Nanomaterials, Polizu Street no 1-7, 011061 Bucharest, Romania.
authors.forename: Alexandru Mihai
authors.initials: AM
authors.lastname: Grumezescu
authors.affiliation: Politehnica University of Bucharest, Faculty of Applied Chemistry and Materials Science, Department of Science and Engineering of Oxide Materials and Nanomaterials, Polizu Street no 1-7, 011061 Bucharest, Romania.
authors.forename: Cristina Daniela
authors.initials: CD
authors.lastname: Ghitulica
authors.affiliation: Politehnica University of Bucharest, Faculty of Applied Chemistry and Materials Science, Department of Science and Engineering of Oxide Materials and Nanomaterials, Polizu Street no 1-7, 011061 Bucharest, Romania.
authors.forename: Georgeta
authors.initials: G
authors.lastname: Voicu
authors.affiliation: The School of Chinese Medicine for Post-Baccalaureate, I-Shou University, Kaohsiung 82445, Taiwan.
authors.forename: Keng-Shiang
authors.initials: KS
authors.lastname: Huang
authors.affiliation: Department of Biological Science and Technology, I-Shou University, Taiwan.
authors.forename: Chih-Hui
authors.initials: CH
authors.lastname: Yang
authors.affiliation: Politehnica University of Bucharest, Faculty of Applied Chemistry and Materials Science, Department of Science and Engineering of Oxide Materials and Nanomaterials, Polizu Street no 1-7, 011061 Bucharest, Romania. Electronic address: anton_ficai81@yahoo.com.
authors.forename: Anton
authors.initials: A
authors.lastname: Ficai
authors.affiliation: Politehnica University of Bucharest, Faculty of Applied Chemistry and Materials Science, Department of Science and Engineering of Oxide Materials and Nanomaterials, Polizu Street no 1-7, 011061 Bucharest, Romania.
authors.forename: Bogdan Stefan
authors.initials: BS
authors.lastname: Vasile
authors.affiliation: Politehnica University of Bucharest, Faculty of Applied Chemistry and Materials Science, Department of Science and Engineering of Oxide Materials and Nanomaterials, Polizu Street no 1-7, 011061 Bucharest, Romania; Laser-Surface-Plasma Interactions Laboratory, Lasers Department National Institute for Lasers, Plasma, and Radiation Physics, 77125 Magurele, Romania.
authors.forename: Valentina
authors.initials: V
authors.lastname: Grumezescu
authors.affiliation: Stefan S. Nicolau Institute of Virology, 285 Mihai Bravu, 030304 Bucharest, Romania.
authors.forename: Coralia
authors.initials: C
authors.lastname: Bleotu
authors.affiliation: University of Bucharest, Faculty of Biology, Microbiology Immunology Department, Aleea Portocalelor no 1-3, 060101 Bucharest, Romania.
authors.forename: Mariana Carmen
authors.initials: MC
authors.lastname: Chifiriuc
mesh_terms: D000287:Administration, Topical; D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000737:chemistry*; D001414:Bacitracin / Q000008:administration & dosage / Q000737:chemistry; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004337:Drug Carriers / Q000008:administration & dosage / Q000737:chemistry*; D006801:Humans; D007612:Kanamycin / Q000008:administration & dosage / Q000737:chemistry; D008826:Microbial Sensitivity Tests; D046529:Microscopy, Electron, Transmission; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D012822:Silicon Dioxide / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D013207:Staphylococcal Skin Infections / Q000188:drug therapy; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000254:growth & development; D013499:Surface Properties; D014961:X-Ray Diffraction; D005680:gamma-Aminobutyric Acid / Q000737:chemistry*
chemical_list: D000900:Anti-Bacterial Agents; D004337:Drug Carriers; D001414:Bacitracin; D005680:gamma-Aminobutyric Acid; D007612:Kanamycin; D012822:Silicon Dioxide
keywords: Anti-staphylococcal activity; Gamma-aminobutyric acid; Improved MIC; Silica nanopowder