title: Effect of genetic variations within the SH2B2 gene on the growth of Chinese cattle.
abstract: As an adaptor protein, apart from potentiating Janus kinase 2 (JAK2) activation and promoting the insulin signaling pathway, Src homology 2 B 2 (SH2B2) indirectly takes part in the regulation of glucose uptake through the c-Cb1/CAP/TC10 pathway, which can in turn affect growth. In this study, we identified a 4bp indel mutation and three single nucleotide polymorphisms (SNPs) within the SH2B2 gene in 959 individuals from five Chinese cattle breeds by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and sequencing. Based on the four variations, 12 haplotypes were identified. Additionally, there was a tendency that in every population pair-wise linkages increased progressively from V1-V2 to V3-V4. By association analysis, positive effects of genotypes CC and CT (snp1220 locus), DI (4bp indel locus), and CC (snp21049 locus) on growth traits were obtained. Furthermore, when combined, individuals with the combination CCDITTCC showed the best performance at an early age. These results were suggestive of an association of snp1220, 4bp indel and snp12049 with growth performance in Nanyang cattle, indicating possibly the candidate role of the SH2B2 gene in marker assisted selection in a beef cattle breeding program.
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling, Shaanxi 712100, PR China.
authors.forename: Mingjuan
authors.initials: M
authors.lastname: Yang
authors.forename: Junhui
authors.initials: J
authors.lastname: Fu
authors.forename: Xianyong
authors.initials: X
authors.lastname: Lan
authors.forename: Yujia
authors.initials: Y
authors.lastname: Sun
authors.forename: Chuzhao
authors.initials: C
authors.lastname: Lei
authors.forename: Chunlei
authors.initials: C
authors.lastname: Zhang
authors.forename: Hong
authors.initials: H
authors.lastname: Chen
mesh_terms: D033942:Adaptor Proteins, Vesicular Transport / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D001947:Breeding; D002417:Cattle / Q000235:genetics* / Q000254:growth & development; D002681:China; D056726:Genetic Association Studies; D056426:Genetic Loci; D006239:Haplotypes; D054643:INDEL Mutation; D015810:Linkage Disequilibrium; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D017422:Sequence Analysis, DNA
chemical_list: D033942:Adaptor Proteins, Vesicular Transport
keywords: APS; Association analysis; Bovine; HWE; Hardy–Weinberg equilibrium; JAK2; Janus kinase 2; LD; PCR-RFLP; PIC; Polymorphism; SH2B; SH2B2; SNPs; Src homology 2 B; Src homology 2 B 2; adapter protein with a PH and SH2 domain; linkage disequilibrium; polymerase chain reaction-restriction fragment length polymorphism; polymorphism information content; single nucleotide polymorphisms