title: Depolarization-induced suppression of a glycinergic synapse in the superior olivary complex by endocannabinoids.
abstract: The neuronal endocannabinoid system is known to depress synaptic inputs retrogradely in an activity-dependent manner. This mechanism has been generally described for excitatory glutamatergic and inhibitory GABAergic synapses. Here, we report that neurones in the auditory brainstem of the Mongolian gerbil (Meriones unguiculatus) retrogradely regulate the strength of their inputs via the endocannabinoid system. By means of whole-cell patch-clamp recordings, we found that retrograde endocannabinoid signalling attenuates both glycinergic and glutamatergic post-synaptic currents in the same types of neurones. Accordingly, we detected the cannabinoid receptor 1 in excitatory and inhibitory pre-synapses as well as the endocannabinoid-synthesising enzymes (diacylglycerol lipase α/β, DAGLα/β) post-synaptically through immunohistochemical stainings. Our study was performed with animals aged 10-15 days, that is, in the time window around the onset of hearing. Therefore, we suggest that retrograde endocannabinoid signalling has a role in adapting inputs during the functionally important switch from spontaneously generated to sound-related signals.
authors.affiliation: Department of Biology II, Division of Neurobiology, Ludwig Maximilians University Munich, Martinsried, Germany; Graduate School of Systemic Neurosciences, Ludwig Maximilians University Munich, Martinsried, Germany.
authors.forename: Barbara
authors.initials: B
authors.lastname: Trattner
authors.forename: Sarah
authors.initials: S
authors.lastname: Berner
authors.forename: Benedikt
authors.initials: B
authors.lastname: Grothe
authors.forename: Lars
authors.initials: L
authors.lastname: Kunz
mesh_terms: D000818:Animals; D004558:Electric Stimulation; D055724:Electrophysiological Phenomena; D063388:Endocannabinoids / Q000494:pharmacology*; D005260:Female; D005849:Gerbillinae; D005998:Glycine / Q000502:physiology*; D007150:Immunohistochemistry; D008071:Lipoprotein Lipase / Q000378:metabolism; D008297:Male; D009847:Olivary Nucleus / Q000187:drug effects*; D043884:Receptor, Cannabinoid, CB1 / Q000187:drug effects; D013569:Synapses / Q000187:drug effects*; D009435:Synaptic Transmission / Q000187:drug effects
chemical_list: D063388:Endocannabinoids; D043884:Receptor, Cannabinoid, CB1; D008071:Lipoprotein Lipase; D005998:Glycine
keywords: CB1; DSE; DSI; LSO; MSO; retrograde