title: High-resolution live-cell imaging and time-lapse microscopy of invadopodium dynamics and tracking analysis.
abstract: Invadopodia are specialized structures of cancer cells which aid in cancer cell invasion and metastasis. Therefore, studying the early steps of invadopodium assembly and its life cycle at the subcellular level by using high spatiotemporal resolution imaging provides an opportunity for understanding the signaling mechanisms involved in this very important process. In this chapter, we describe the design of a custom-built high-resolution fluorescence microscope which makes this challenging imaging possible. We also describe an ImageJ plugin that we have developed for tracking of invadopodia and lifetime analysis.
authors.affiliation: Department of Anatomy and Structural Biology and Gruss Lipper Biophotonics Center, Albert Einstein College of Medicine, Bronx, NY, USA.
authors.forename: Ved P
authors.initials: VP
authors.lastname: Sharma
authors.forename: David
authors.initials: D
authors.lastname: Entenberg
authors.forename: John
authors.initials: J
authors.lastname: Condeelis
mesh_terms: D002462:Cell Membrane / Q000648:ultrastructure*; D058948:Cell Tracking; D006801:Humans; D008856:Microscopy, Fluorescence; D008967:Molecular Biology / Q000379:methods*; D057054:Molecular Imaging / Q000379:methods*; D009362:Neoplasm Metastasis / Q000473:pathology; D009369:Neoplasms / Q000473:pathology; D015398:Signal Transduction