title: Hepatitis B virus genetic variation and TP53 R249S mutation in patients with hepatocellular carcinoma in Thailand.
abstract: Chronic hepatitis B virus (HBV) infection and dietary exposure to aflatoxin B1 (AFB1) are major risk factors for hepatocellular carcinoma (HCC). The aim of this study was to evaluate the role of HBV genetic variation and the R249S mutation of the p53 gene, a marker of AFB1-induced HCC, in Thai patients chronically infected with HBV. Sixty-five patients with and 89 patients without HCC were included. Viral mutations and R249S mutation were characterized by direct sequencing and restriction fragment length polymorphism (RFLP) in serum samples, respectively. The prevalences of T1753C/A/G and A1762T/G1764A mutations in the basal core promotor (BCP) region were significantly higher in the HCC group compared to the non-HCC group. R249S mutation was detected in 6.2% and 3.4% of the HCC and non-HCC groups, respectively, which was not significantly different. By multiple logistic regression analysis, the presence of A1762T/G1764A mutations was independently associated with the risk of HCC in Thai patients.
authors.affiliation: Department of Biochemistry, Chulalongkorn University, Bangkok, Thailand.
authors.forename: Chureeporn
authors.initials: C
authors.lastname: Thongbai
authors.forename: Pattaratida
authors.initials: P
authors.lastname: Sa-nguanmoo
authors.forename: Pavanrat
authors.initials: P
authors.lastname: Kranokpiruk
authors.forename: Kittiyod
authors.initials: K
authors.lastname: Poovorawan
authors.forename: Yong
authors.initials: Y
authors.lastname: Poovorawan
authors.forename: Pisit
authors.initials: P
authors.lastname: Tangkijvanich
mesh_terms: D000328:Adult; D006528:Carcinoma, Hepatocellular / Q000209:etiology* / Q000473:pathology; D016022:Case-Control Studies; D004279:DNA, Viral / Q000235:genetics*; D005260:Female; D005500:Follow-Up Studies; D014644:Genetic Variation; D005838:Genotype; D006515:Hepatitis B virus / Q000145:classification / Q000235:genetics*; D019694:Hepatitis B, Chronic / Q000150:complications* / Q000235:genetics / Q000821:virology; D006801:Humans; D008113:Liver Neoplasms / Q000209:etiology* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009154:Mutation / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D011379:Prognosis; D012307:Risk Factors; D013785:Thailand; D016159:Tumor Suppressor Protein p53 / Q000235:genetics*
chemical_list: D004279:DNA, Viral; C495901:TP53 protein, human; D016159:Tumor Suppressor Protein p53