title: Pharmacokinetics of dexamethasone after intravenous and intramuscular administration in pigs.
abstract: The pharmacokinetics of dexamethasone (DEX) were investigated after an intravenous (IV) or intramuscular (IM) bolus injection of 0.3mg/kg bodyweight DEX sodium phosphate in pigs. The plasma concentrations of DEX were determined using a validated high-performance liquid chromatography-tandem mass spectrometry (LC-MS/MS) method and the pharmacokinetics were determined by one-compartmental analysis. The mean area under the plasma concentration-time curve and the mean elimination half-life were 133.07 ± 39.59 ng.h/mL and 0.77 h, and 173.24 ± 53.59 ngh/mL and 1.06 h following IV and IM administration, respectively. The volume of distribution and clearance recorded after IV administration were 2.78 ± 0.88 L/kg and 2.39 ± 0.57 L/hkg, respectively. An IM bolus injection of DEX sodium phosphate in pigs resulted in a fast and complete absorption, with a mean maximal plasma concentration of 80.94 ± 21.29 ng/mL at 0.35 ± 0.21 h and a high absolute bioavailability of 131.06 ± 26.05%.
authors.affiliation: Department of Pharmacology, Toxicology and Biochemistry, Ghent University, Faculty of Veterinary Medicine, Salisburylaan 133, 9820 Merelbeke, Belgium. Electronic address: Heidi.Wyns@UGent.be.
authors.forename: Heidi
authors.initials: H
authors.lastname: Wyns
authors.forename: Evelyne
authors.initials: E
authors.lastname: Meyer
authors.forename: Anneleen
authors.initials: A
authors.lastname: Watteyn
authors.forename: Elke
authors.initials: E
authors.lastname: Plessers
authors.forename: Siegrid
authors.initials: S
authors.lastname: De Baere
authors.forename: Patrick
authors.initials: P
authors.lastname: De Backer
authors.forename: Siska
authors.initials: S
authors.lastname: Croubels
mesh_terms: D000818:Animals; D019540:Area Under Curve; D001682:Biological Availability; D002853:Chromatography, Liquid; D003907:Dexamethasone / Q000031:analogs & derivatives* / Q000097:blood / Q000493:pharmacokinetics; D005260:Female; D005938:Glucocorticoids / Q000097:blood / Q000493:pharmacokinetics*; D007273:Injections, Intramuscular / Q000662:veterinary; D007275:Injections, Intravenous / Q000662:veterinary; D034421:Sus scrofa / Q000378:metabolism*; D053719:Tandem Mass Spectrometry
chemical_list: D005938:Glucocorticoids; C004180:dexamethasone 21-phosphate; D003907:Dexamethasone
keywords: Bioavailability; Dexamethasone; Intramuscular; Pharmacokinetics; Pig