title: Synthesis and one-pot tethering of hydroxyl-capped phosphorylcholine onto cellulose membrane for improving hemocompatibility and antibiofouling property.
abstract: Tethering of biomimetic phosphorylcholine derivative onto the surface of biomedical devices is an effective method for improving hemocompatibility and antibiofouling property. Herein, series of novel hydroxyl-capped phosphorylcholines (HOPC) with different carbon spacer lengths were first synthesized and characterized with element analysis (EA), Fourier transform infrared spectroscopy(FTIR), and nuclear magnetic resonance spectroscopy (NMR). Then, HOPC (n=5, 2a) was one-pot tethered onto cellulose membrane with hexamethylene diisocyanate (HDI) as a coupling agent. The existence of phosphorylcholine was demonstrated by water contact angle measurement, attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR), atomic force microscopy (AFM), and X-ray photoelectron spectroscopy (XPS). The hemocompatibility and antibiofouling property were evaluated by hemolytic test, platelet adhesion, protein adsorption, and Escherichia coli adhesion test. The results showed that cellulose membranes tethered with HOPC exhibited excellent hemocompatibility featured by low platelet adhesion and ﬁbrinogen adsorption as well as antibiofouling property with bacterial adhesion resistance.
authors.affiliation: Jiangsu Key Laboratory of Biofunctional Materials, College of Chemistry and Materials Science, Nanjing Normal University, Nanjing 210023, China; Jiangsu Technological Research Center for Interfacial Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, China.
authors.forename: Jiang
authors.initials: J
authors.lastname: Yuan
authors.affiliation: Jiangsu Technological Research Center for Interfacial Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Tong
authors.affiliation: Jiangsu Key Laboratory of Biofunctional Materials, College of Chemistry and Materials Science, Nanjing Normal University, Nanjing 210023, China.
authors.forename: Huaixiang
authors.initials: H
authors.lastname: Yi
authors.affiliation: Jiangsu Key Laboratory of Biofunctional Materials, College of Chemistry and Materials Science, Nanjing Normal University, Nanjing 210023, China. Electronic address: wangbingxiang@njnu.edu.cn.
authors.forename: Bingxiang
authors.initials: B
authors.lastname: Wang
authors.affiliation: Jiangsu Key Laboratory of Biofunctional Materials, College of Chemistry and Materials Science, Nanjing Normal University, Nanjing 210023, China; Jiangsu Technological Research Center for Interfacial Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, China. Electronic address: jshen@njnu.edu.cn.
authors.forename: Jian
authors.initials: J
authors.lastname: Shen
authors.affiliation: Jiangsu Technological Research Center for Interfacial Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, China.
authors.forename: Sicong
authors.initials: S
authors.lastname: Lin
mesh_terms: D000327:Adsorption; D000818:Animals; D001422:Bacterial Adhesion / Q000187:drug effects; D057219:Biofouling / Q000517:prevention & control*; D002417:Cattle; D002482:Cellulose / Q000737:chemistry*; D004926:Escherichia coli / Q000187:drug effects; D005340:Fibrinogen / Q000378:metabolism; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D006900:Hydroxylation; D008422:Materials Testing / Q000379:methods*; D008567:Membranes, Artificial; D018625:Microscopy, Atomic Force; D009943:Organophosphorus Compounds / Q000138:chemical synthesis / Q000737:chemistry; D010767:Phosphorylcholine / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology*; D056951:Photoelectron Spectroscopy; D010973:Platelet Adhesiveness / Q000187:drug effects; D053657:Platelet-Rich Plasma / Q000378:metabolism; D066244:Proton Magnetic Resonance Spectroscopy
chemical_list: D008567:Membranes, Artificial; D009943:Organophosphorus Compounds; D010767:Phosphorylcholine; D005340:Fibrinogen; D002482:Cellulose
keywords: Antibiofouling; Cellulose; Hemocompatibility; Phosphorylcholine derivative; Synthesis