title: Preparation of 2-nitro-5-thiobenzoate for the routine determination of reagent hypochlorous acid concentrations.
abstract: Solutions of hypochlorous acid (HOCl) decay over time. This decay indicates the necessity for methods and reagents for the routine measurement of this oxidant. 2-Nitro-5-thiobenzoate is commonly used to measure HOCl concentrations. This article describes a method for the preparation of 2-nitro-5-thiobenzoate that is stable for at least 3 months. This method relies on the partial rather than full reduction of 5,5'-dithiobis-(2-nitrobenzoic acid) and the resulting equilibrium between the substrate and the product.
authors.affiliation: Winthrop University Hospital, Mineola, NY 11501, USA. Electronic address: Tjeitner@Winthrop.org.
authors.forename: Thomas M
authors.initials: TM
authors.lastname: Jeitner
authors.forename: Mike
authors.initials: M
authors.lastname: Kalogiannis
authors.forename: Jim
authors.initials: J
authors.lastname: Mathew
mesh_terms: D004228:Dithionitrobenzoic Acid / Q000737:chemistry*; D006997:Hypochlorous Acid / Q000032:analysis*; D016877:Oxidants / Q000737:chemistry; D010084:Oxidation-Reduction; D012996:Solutions
chemical_list: D016877:Oxidants; D012996:Solutions; D006997:Hypochlorous Acid; D004228:Dithionitrobenzoic Acid
keywords: 2-Nitro-5-thiobenzoate; Ellman’s reagent; Hypochlorous acid