title: Development of buffers for fast semidry transfer of proteins.
abstract: Western blot is an extensively used method for protein detection in cell biology. To optimize this procedure, here we examined a panel of buffers for their ability to efficiently transfer proteins from SDS-polyacrylamide gels onto nitrocellulose membranes in a short 12-min period, designated here as fast semidry transfer. Our results show for the first time that HEPES- and HEPPS/EPPS-based buffers represent the most efficient buffers for fast semidry transfer.
authors.affiliation: Division of Hematology, Department of Medicine, Royal Victoria Hospital, McGill University Health Centre, McGill University, Montreal, QC H3A 1A1, Canada.
authors.forename: Dušan
authors.initials: D
authors.lastname: Garić
authors.forename: Laure
authors.initials: L
authors.lastname: Humbert
authors.forename: Nadège
authors.initials: N
authors.lastname: Fils-Aimé
authors.forename: Juliana
authors.initials: J
authors.lastname: Korah
authors.forename: Yasaman
authors.initials: Y
authors.lastname: Zarfabian
authors.forename: Jean-Jacques
authors.initials: JJ
authors.lastname: Lebrun
authors.forename: Suhad
authors.initials: S
authors.lastname: Ali
mesh_terms: D002021:Buffers; D003101:Collodion / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel / Q000191:economics / Q000379:methods*; D006531:HEPES / Q000737:chemistry; D008567:Membranes, Artificial; D011506:Proteins / Q000302:isolation & purification*; D013997:Time Factors
chemical_list: D002021:Buffers; D008567:Membranes, Artificial; D011506:Proteins; D003101:Collodion; D006531:HEPES
keywords: HEPES; HEPPS; Protein transfer; Western blot