title: Temperature influences β-carotene production in recombinant Saccharomyces cerevisiae expressing carotenogenic genes from Phaffia rhodozyma.
abstract: Red yeast Phaffia rhodozyma is a prominent microorganism able to synthesize carotenoid. Here, three carotenogenic cDNAs of P. rhodozyma CGMCC 2.1557, crtE, crtYB and crtI, were cloned and introduced into Saccharomyces cerevisiae INVSc1. The recombinant Sc-EYBI cells could synthesize 258.8 ± 43.8 μg g(-1) dry cell weight (DCW) of β-carotene when growing at 20 °C, about 59-fold higher than in those growing at 30 °C. Additional expression of the catalytic domain of 3-hydroxy-3-methylglutaryl-coenzyme A reductase from S. cerevisiae (Sc-EYBIH) increased the β-carotene level to 528.8 ± 13.3 μg g(-1) DCW as cells growing at 20 °C, 27-fold higher than cells growing at 30 °C, although cells grew faster at 30 °C than at 20 °C. Consistent with the much higher β-carotene level in cells growing at 20 °C, transcription level of three crt genes and cHMG1 gene in cells growing at 20 °C was a little higher than in those growing at 30 °C. Meanwhile, expression of three carotenogenic genes and accumulation of β-carotene promoted cell growth. These results reveal the influence of temperature on β-carotene biosynthesis and may be helpful for improving β-carotene production in recombinant S. cerevisiae.
authors.affiliation: State Key Laboratory of Food Science and Technology, Jiangnan University, 1800 Lihu Avenue, Wuxi, 214122, China, shifeng@jiangnan.edu.cn.
authors.forename: Feng
authors.initials: F
authors.lastname: Shi
authors.forename: Wubing
authors.initials: W
authors.lastname: Zhan
authors.forename: Yongfu
authors.initials: Y
authors.lastname: Li
authors.forename: Xiaoyuan
authors.initials: X
authors.lastname: Wang
mesh_terms: D001487:Basidiomycota / Q000201:enzymology* / Q000235:genetics; D053898:Biosynthetic Pathways / Q000235:genetics*; D020869:Gene Expression Profiling; D060847:Metabolic Engineering; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics / Q000378:metabolism* / Q000528:radiation effects*; D013696:Temperature; D019207:beta Carotene / Q000096:biosynthesis*
chemical_list: D011994:Recombinant Proteins; D019207:beta Carotene