title: Deletions within the 3' non-translated region of Alfalfa mosaic virus RNA4 do not affect replication but significantly reduce long-distance movement of chimeric Tobacco mosaic virus.
abstract: Alfalfa mosaic virus (AlMV) RNAs 1 and 2 with deletions in their 3' non‑translated regions (NTRs) have been previously shown to be encapsidated into virions by coat protein (CP) expressed from RNA3, indicating that the 3' NTRs of RNAs 1 and 2 are not required for virion assembly. Here, we constructed various mutants by deleting sequences within the 3' NTR of AlMV subgenomic (sg) RNA4 (same as of RNA3) and examined the effect of these deletions on replication and translation of chimeric Tobacco mosaic virus (TMV) expressing AlMV sgRNA4 from the TMV CP sg promoter (Av/A4) in tobacco protoplasts and Nicotiana benthamiana plants. While the Av/A4 mutants were as competent as the wild-type Av/A4 in RNA replication in protoplasts, their encapsidation, long-distance movement and virus accumulation varied significantly in N. benthamiana. These data suggest that the 3' NTR of AlMV sgRNA4 contains potential elements necessary for virus encapsidation.
authors.affiliation: Fraunhofer USA Center for Molecular Biotechnology, 9 Innovation Way, Newark, DE 19711, USA.
authors.forename: Gourgopal
authors.initials: G
authors.lastname: Roy
authors.forename: Oleg
authors.initials: O
authors.lastname: Fedorkin
authors.forename: Masaaki
authors.initials: M
authors.lastname: Fujiki
authors.forename: Marina
authors.initials: M
authors.lastname: Skarjinskaia
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Knapp
authors.forename: Shailaja
authors.initials: S
authors.lastname: Rabindran
authors.forename: Vidadi
authors.initials: V
authors.lastname: Yusibov
mesh_terms: D020413:3' Untranslated Regions; D017794:Alfalfa mosaic virus / Q000502:physiology*; D015870:Gene Expression; D054884:Host-Pathogen Interactions; D010944:Plants; D012367:RNA, Viral / Q000235:genetics*; D011995:Recombination, Genetic; D017384:Sequence Deletion; D014026:Nicotiana / Q000821:virology; D014027:Tobacco Mosaic Virus / Q000235:genetics; D019065:Virus Assembly; D014779:Virus Replication
chemical_list: D020413:3' Untranslated Regions; D012367:RNA, Viral