title: Evidence for the antagonistic form of CXC-motif chemokine CXCL10 in serous epithelial ovarian tumours.
abstract: Patients with high-grade, serous epithelial ovarian carcinoma (HGSOC) are generally diagnosed with extensive peritoneal metastases, and exhibit 5-year survival rates <30%. A subset of these tumours, defined as "immunoreactive," overexpress mRNA encoding the T-cell-recruiting chemokine CXCL10 (10-kDa interferon gamma-induced protein; C-X-C motif chemokine 10). Tumour-infiltrating CD4(+) CD8(+) T-cells are a well-documented, positive prognostic indicator for HGSOC patients; paradoxically, however, patients diagnosed with HGSOC (overexpressing CXCL10 and therefore theorised to recruit T-cells) typically exhibit poor survival. Recently, an "antagonistic" CXCL10 variant was identified that inhibited leucocyte recruitment to inflamed liver in vivo (Casrouge et al., J Clin Invest 2011;121:308-17). We hypothesised that "immunoreactive" HGSOC might also express antagonistic CXCL10, interfering with leucocyte recruitment and contributing to poor patient prognosis. CXCL10 expression was analysed in HGSOC tissues grouped according to pathology, grade and FIGO stage at diagnosis, and its localisation and association with T-cells established by immunohistochemical staining in tissue microarrays. CXCL10 expression was increased in a subset of serous epithelial tumour samples; however, it did not correlate well with CD45-positive tumour infiltrate. Immunoprecipitation and de novo sequence analysis of CXCL10 identified the N-terminally cleaved, "antagonistic" variant of CXCL10 specifically in malignant tumours, and not in benign ovarian disease. The data demonstrate the presence of the antagonistic form of CXCL10 in HGSOC for the first time, and provide a partial explanation for reduced leucocyte infiltration observed in these tumours. We suggest that CXCL10 cleavage and subsequent antagonism of immune cell recruitment may be a feature of the "immunoreactive" HGSOC subtype, leading to early impairment of the immune response and subsequently worsening patient prognosis.
authors.affiliation: Ovarian Cancer Biomarker Laboratory, Prince Henry's Institute of Medical Research, Clayton, VIC, Australia.
authors.forename: Adam
authors.initials: A
authors.lastname: Rainczuk
authors.forename: Jyothsna R
authors.initials: JR
authors.lastname: Rao
authors.forename: Jessica L
authors.initials: JL
authors.lastname: Gathercole
authors.forename: Nicole J
authors.initials: NJ
authors.lastname: Fairweather
authors.forename: Simon
authors.initials: S
authors.lastname: Chu
authors.forename: Rina
authors.initials: R
authors.lastname: Masadah
authors.forename: Thomas W
authors.initials: TW
authors.lastname: Jobling
authors.forename: Santanu
authors.initials: S
authors.lastname: Deb-Choudhury
authors.forename: Jolon
authors.initials: J
authors.lastname: Dyer
authors.forename: Andrew N
authors.initials: AN
authors.lastname: Stephens
mesh_terms: D000328:Adult; D000595:Amino Acid Sequence; D000077216:Carcinoma, Ovarian Epithelial; D054357:Chemokine CXCL10 / Q000097:blood / Q000737:chemistry / Q000378:metabolism* / Q000652:urine; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008875:Middle Aged; D008969:Molecular Sequence Data; D009375:Neoplasms, Glandular and Epithelial / Q000378:metabolism*; D010051:Ovarian Neoplasms / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction
chemical_list: C114753:CXCL10 protein, human; D054357:Chemokine CXCL10
keywords: CXCL10; dipeptidyl peptidase; leucocyte; mass spectrometry; ovarian cancer