title: Rapid detection of melamine adulteration in dairy milk by SB-ATR-Fourier transform infrared spectroscopy.
abstract: Melamine is a nitrogenous chemical substance used principally as a starting material for the manufacture of synthetic resins. Due to its very high proportion of nitrogen melamine has been added illegitimately to foods and feeds to increase the measured protein content, which determines the value of the product. These issues prompted private as well as governmental laboratories to develop methods for the analysis of melamine in a wide variety of food products and ingredients. Owing to this fact present study is aimed to use single bounce attenuated total reflectance (SB-ATR) Fourier transform infrared spectroscopy (FTIR) method as an effective rapid tool for the detection and quantification of melamine in milk (liquid and powder). Partial least-squares (PLS) models were established for correlating spectral data to melamine concentration with R(2)>0.99, and RMSEC 0.370. Linear calibration curves were obtained over the calibration range of 25-0.0625%. The LOD and LOQ of the method was 0.00025% (2.5 ppm) and 0.0015% (15 ppm) respectively. Proposed SB-ATR-FTIR method requires little or no sample preparation with an assay time of 1-2 min.
authors.affiliation: National Center of Excellence in Analytical Chemistry, University of Sindh, Jamshoro 76080, Pakistan.
authors.forename: Sana
authors.initials: S
authors.lastname: Jawaid
authors.forename: Farah N
authors.initials: FN
authors.lastname: Talpur
authors.forename: S T H
authors.initials: ST
authors.lastname: Sherazi
authors.forename: Shafi M
authors.initials: SM
authors.lastname: Nizamani
authors.forename: Abid A
authors.initials: AA
authors.lastname: Khaskheli
mesh_terms: D000818:Animals; D002417:Cattle; D005506:Food Contamination / Q000032:analysis*; D008892:Milk / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*; D014227:Triazines / Q000032:analysis*
chemical_list: D014227:Triazines; C011907:melamine
keywords: Adulteration; Dairy milk; Melamine; SB-ATR–FTIR spectroscopy