title: Efficient arachidonic acid-rich oil production by Mortierella alpina through a three-stage fermentation strategy.
abstract: A three-stage fermentation strategy was designed for efficient arachidonic acid (ARA)-rich oil production by Mortierella alpina. The process at different stages by changing the components of medium was investigated. In the first stage, mycelia were inoculated in a nutrient-rich medium for rapid propagation. In the second stage, mycelia were collected and then cultivated in glucose solution to achieve high cellular lipid contents. In the third stage, mycelia were cultured in a glucose-absent medium to obtain rapid ARA accumulation. Using this fermentation strategy, high dry cell weight, lipid, and ARA concentration reached 41.6, 26.6, and 11.4 g/L, respectively. The results demonstrated that mycelia propagation, lipid biosynthesis, and ARA accumulation process can be significantly spatially separated, allowing further optimization to improve the efficiency of each stage. This was the first report of using a three-stage fermentation strategy for ARA-rich oil production, and it could be applied to other similar oleaginous microorganisms to obtain high related polyunsaturated fatty acids accumulation.
authors.affiliation: State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, No. 5 Xinmofan Road, Nanjing, 210009, People's Republic of China.
authors.forename: Zhi-Kui
authors.initials: ZK
authors.lastname: Nie
authors.forename: Zhong-Tao
authors.initials: ZT
authors.lastname: Deng
authors.forename: Ai-Hui
authors.initials: AH
authors.lastname: Zhang
authors.forename: Xiao-Jun
authors.initials: XJ
authors.lastname: Ji
authors.forename: He
authors.initials: H
authors.lastname: Huang
mesh_terms: D016718:Arachidonic Acid / Q000096:biosynthesis*; D003470:Culture Media; D005285:Fermentation; D005947:Glucose / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D020094:Mortierella / Q000378:metabolism*
chemical_list: D003470:Culture Media; D016718:Arachidonic Acid; D005947:Glucose