title: Full-length sequence analysis of hepatitis E virus isolates: showing potential determinants of virus genotype and identity.
abstract: The complete genome sequence of a genotype 4 strain of hepatitis E virus (CH-YT-HEV02) from a patient (in Yantai, China) has been determined. Phylogenetic analysis showed that CH-YT-HEV02 belongs to genotype 4, subtype 4a. However, the phylogenetic analysis indicated that it was most closely related to JKO-CHiSai98C (AB197673) strain, sharing only 91.6% sequence identity with it. Judging from the phylogenetic tree based on the full-length nucleotide sequences of all 70 genotype 4 HEV isolates retrieved from GenBank up to May, 2013, the CH-YT-HEV02 isolates could serve as a Yantai-indigenous strain. A broader comparison with other genotype isolates revealed that there are a few conserved amino acids in the HVR region of different HEV genotypes, and two amino acid motifs in ORF2 and ORF3 might serve as signatures of genotype diversity of HEV.
authors.affiliation: Department of Environment and Health, Institute of Health and Environmental Medicine, Key Laboratory of Risk Assessment and Control for Environment and Food Safety, No. 1, Dali Road, Tianjin, 300050, China.
authors.forename: Dong
authors.initials: D
authors.lastname: Yang
authors.forename: Mei
authors.initials: M
authors.lastname: Jiang
authors.forename: Min
authors.initials: M
authors.lastname: Jin
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Qiu
authors.forename: Weihong
authors.initials: W
authors.lastname: Cui
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Shen
authors.forename: Bo
authors.initials: B
authors.lastname: Li
authors.forename: Lianfeng
authors.initials: L
authors.lastname: Gong
authors.forename: Zhaoli
authors.initials: Z
authors.lastname: Chen
authors.forename: Xinwei
authors.initials: X
authors.lastname: Wang
authors.forename: Jun-Wen
authors.initials: JW
authors.lastname: Li
mesh_terms: D000328:Adult; D000595:Amino Acid Sequence; D001483:Base Sequence; D002681:China; D005243:Feces / Q000821:virology; D016679:Genome, Viral; D016751:Hepatitis E / Q000821:virology*; D016752:Hepatitis E virus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D020539:Sequence Analysis, Protein