title: The transcription factor protein Sox11 enhances early osteoblast differentiation by facilitating proliferation and the survival of mesenchymal and osteoblast progenitors.
abstract: Sox11 deletion mice are known to exhibit developmental defects of craniofacial skeletal malformations, asplenia, and hypoplasia of the lung, stomach, and pancreas. Despite the importance of Sox11 in the developing skeleton, the role of Sox11 in osteogenesis has not been studied yet. In this study, we identified that Sox11 is an important transcription factor for regulating the proliferation and survival of osteoblast precursor cells as well as the self-renewal potency of mesenchymal progenitor cells via up-regulation of Tead2. Furthermore, Sox11 also plays an important role in the segregation of functional osteoblast lineage progenitors from osteochondrogenic progenitors. Facilitation of osteoblast differentiation from mesenchymal cells was achieved by enhanced expression of the osteoblast lineage specific transcription factors Runx2 and Osterix. Morpholino-targeted disruption of Sox11 in zebrafish impaired organogenesis, including the bones, which were under mineralized. These results indicated that Sox11 plays a crucial role in the proliferation and survival of mesenchymal and osteoblast precursors by Tead2, and osteogenic differentiation by regulating Runx2 and Osterix.
authors.affiliation: From the Division of Endocrinology and Endocrine Research Institute, Department of Internal Medicine.
authors.forename: Jogeswar
authors.initials: J
authors.lastname: Gadi
authors.affiliation: the Diabetic Complications Research Center, Division of Traditional Korean Medicine (TKM), Integrated Research, Korea Institute of Oriental Medicine (KIOM), Daejeon, Korea 305-811; the Laboratory of Developmental Genetics, Department of Biology, Chungnam National University, Daejeon, Korea 305-764.
authors.forename: Seung-Hyun
authors.initials: SH
authors.lastname: Jung
authors.affiliation: the Brain Korea 21 Project for Medical Sciences,; the Division of Anatomy and Developmental Biology, Department of Oral Biology, Research Center for Orofacial Hard Tissue Regeneration, College of Dentistry, Yonsei University, Seoul, Korea 120-752, and.
authors.forename: Min-Jung
authors.initials: MJ
authors.lastname: Lee
authors.affiliation: From the Division of Endocrinology and Endocrine Research Institute, Department of Internal Medicine,; the Brain Korea 21 Project for Medical Sciences.
authors.forename: Ajita
authors.initials: A
authors.lastname: Jami
authors.affiliation: the Brain Korea 21 Project for Medical Sciences,; the Department of Anatomy, Embryology Lab, Yonsei University College of Medicine, Seoul, Korea 120-752.
authors.forename: Kalyani
authors.initials: K
authors.lastname: Ruthala
authors.affiliation: From the Division of Endocrinology and Endocrine Research Institute, Department of Internal Medicine,; the Brain Korea 21 Project for Medical Sciences.
authors.forename: Kyoung-Min
authors.initials: KM
authors.lastname: Kim
authors.affiliation: the Department of Pathology, and.
authors.forename: Nam-Hoon
authors.initials: NH
authors.lastname: Cho
authors.affiliation: the Division of Anatomy and Developmental Biology, Department of Oral Biology, Research Center for Orofacial Hard Tissue Regeneration, College of Dentistry, Yonsei University, Seoul, Korea 120-752, and.
authors.forename: Han-Sung
authors.initials: HS
authors.lastname: Jung
authors.affiliation: the Laboratory of Developmental Genetics, Department of Biology, Chungnam National University, Daejeon, Korea 305-764.
authors.forename: Cheol-Hee
authors.initials: CH
authors.lastname: Kim
authors.affiliation: From the Division of Endocrinology and Endocrine Research Institute, Department of Internal Medicine,; the Brain Korea 21 Project for Medical Sciences,. Electronic address: lsk@yuhs.ac.
authors.forename: Sung-Kil
authors.initials: SK
authors.lastname: Lim
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000502:physiology*; D002460:Cell Line; D049109:Cell Proliferation; D002470:Cell Survival / Q000502:physiology; D050659:Core Binding Factor Alpha 1 Subunit / Q000235:genetics / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D051379:Mice; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism*; D055751:SOXC Transcription Factors / Q000235:genetics / Q000378:metabolism*; D000076182:Sp7 Transcription Factor; D000091262:TEA Domain Transcription Factors; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D015027:Zebrafish / Q000196:embryology / Q000235:genetics
chemical_list: D050659:Core Binding Factor Alpha 1 Subunit; D004268:DNA-Binding Proteins; C493716:Runx2 protein, mouse; D055751:SOXC Transcription Factors; C490793:Sox11 protein, mouse; D000076182:Sp7 Transcription Factor; C479466:Sp7 protein, mouse; D000091262:TEA Domain Transcription Factors; C486310:Tead2 protein, mouse; D014157:Transcription Factors
keywords: Apoptosis; Bone; Hippo Signaling; Mesenchymal Stem Cells; Osteoblasts; Osteocyte; Osteogenesis; Osterix; Runx2; Sox11