title: Use of whey permeate containing in situ synthesised galacto-oligosaccharides for the growth and preservation of Lactobacillus plantarum.
abstract: Galacto-oligosaccharides (GOS) are prebiotics that have a beneficial effect on human health by promoting the growth of probiotic bacteria in the gut. GOS are commonly produced from lactose in an enzymatic reaction catalysed by β-galactosidase, named transglycosylation. Lactose is the main constituent of whey permeate (WP), normally wasted output from the cheese industry. Therefore, the main goal of this work was to optimise the synthesis of GOS in WP using β-galatosidase from Aspergillus oryzaea. WP and whey permeate enzymatically treated (WP-GOS) were used as culture media of Lactobacillus plantarum 299v. Lb. plantarum 299v attained the stationary phase in approximately 16 h, reaching 3·6 and 4·1×108 CFU/ml in WP and WP-GOS, respectively. The in situ synthesised GOS were not consumed during growth. No significant differences were observed in the growth kinetics of microorganisms in both media. After fermentation, microorganisms were dehydrated by freeze-drying and spray-drying and stored. The recovery of microorganisms after fermentation, dehydration and storage at 4 °C for at least 120 d was above 108 CFU/g. These studies demonstrated that WP is an appropriate substrate for the synthesis of GOS and the obtained product is also adequate as culture medium of Lb. plantarum 299v. The coexistence of GOS and dehydrated viable probiotic microorganisms, prepared using an effluent as raw material, represents the main achievement of this work, with potential impact in the development of functional foods.
authors.affiliation: Centro de Investigación y Desarrollo en Criotecnología de Alimentos CIDCA Conicet La Plata, UNLP, 1900 La Plata, Argentina.
authors.forename: Marina
authors.initials: M
authors.lastname: Golowczyc
authors.forename: Carlos
authors.initials: C
authors.lastname: Vera
authors.forename: Mauricio
authors.initials: M
authors.lastname: Santos
authors.forename: Cecilia
authors.initials: C
authors.lastname: Guerrero
authors.forename: Paula
authors.initials: P
authors.lastname: Carasi
authors.forename: Andrés
authors.initials: A
authors.lastname: Illanes
authors.forename: Andrea
authors.initials: A
authors.lastname: Gómez-Zavaglia
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Tymczyszyn
mesh_terms: D001236:Aspergillus oryzae / Q000201:enzymology; D058491:Bacterial Load; D002851:Chromatography, High Pressure Liquid; D003470:Culture Media; D005285:Fermentation; D005612:Freeze Drying; D048191:Lactiplantibacillus plantarum / Q000254:growth & development*; D008894:Milk Proteins / Q000378:metabolism*; D009844:Oligosaccharides / Q000032:analysis / Q000378:metabolism*; D056692:Prebiotics; D011309:Preservation, Biological / Q000379:methods; D000067816:Whey Proteins; D001616:beta-Galactosidase / Q000378:metabolism
chemical_list: D003470:Culture Media; D008894:Milk Proteins; D009844:Oligosaccharides; D056692:Prebiotics; D000067816:Whey Proteins; D001616:beta-Galactosidase