title: An anti-sulfatide antibody O4 immunoprecipitates sulfatide rafts including Fyn, Lyn and the G protein α subunit in rat primary immature oligodendrocytes.
abstract: The association of sulfatide with specific proteins in oligodendrocytes was examined by co-immunoprecipitation with an anti-sulfatide antibody. Protein kinase activity was detected in precipitates with a monoclonal antibody to sulfatide (O4) from the rat primary immature oligodendrocytes. We conducted in vitro kinase assay of tyrosine phosphorylated proteins of 80, 59, 56, 53 and 40 kDa by gel electrophoresis. Of these proteins, the proteins of 59 kDa and 53/56 kDa were identified as the Src family tyrosine kinases Fyn and Lyn on the basis of their sequential immunoprecipitation with anti-Fyn and anti-Lyn antibodies, respectively. The 40 kDa protein was identified as the α subunit of the heterotrimeric G protein. These observations suggest that O4 immunoprecipitates sulfatide rafts including Fyn, Lyn and the α subunit of the heterotrimeric G protein.
authors.affiliation: Laboratory of Biomembrane, Tokyo Metropolitan Institute of Medical Science, Kamikitazawa, Setagaya-ku, Tokyo, 156-8506, Japan.
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Miki
authors.forename: Mizuho
authors.initials: M
authors.lastname: Kaneda
authors.forename: Kazuko
authors.initials: K
authors.lastname: Iida
authors.forename: Go
authors.initials: G
authors.lastname: Hasegawa
authors.forename: Makoto
authors.initials: M
authors.lastname: Murakami
authors.forename: Naomasa
authors.initials: N
authors.lastname: Yamamoto
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Asou
authors.forename: Kohji
authors.initials: K
authors.lastname: Kasahara
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D002478:Cells, Cultured; D044385:GTP-Binding Protein alpha Subunits / Q000276:immunology* / Q000378:metabolism; D021962:Membrane Microdomains / Q000276:immunology / Q000378:metabolism; D009836:Oligodendroglia / Q000276:immunology* / Q000378:metabolism; D011485:Protein Binding; D051076:Proto-Oncogene Proteins c-fyn / Q000276:immunology* / Q000378:metabolism; D051381:Rats; D013433:Sulfoglycosphingolipids / Q000276:immunology* / Q000378:metabolism; D019061:src-Family Kinases / Q000276:immunology* / Q000378:metabolism
chemical_list: D000911:Antibodies, Monoclonal; D044385:GTP-Binding Protein alpha Subunits; D013433:Sulfoglycosphingolipids; C494961:Fyn protein, rat; D051076:Proto-Oncogene Proteins c-fyn; C060902:lyn protein-tyrosine kinase; D019061:src-Family Kinases