title: Detection of D2-40 monoclonal antibody-labeled lymphatic vessel invasion in esophageal squamous cell carcinoma and its clinicopathologic significance.
abstract: OBJECTIVE
  This study aims to investigate the clinicopathologic significance of lymphatic vessel invasion (LVI) labeled by D2-40 monoclonal antibody in esophageal squamous cell carcinoma (ESCC).
  
  
  METHODS
  Immunohistochemical assay was used to detect the expression of D2-40 and LVI in 107 ESCC patients. Then, the correlation between the clinicopathologic feature and the overall survival time of the patients was analyzed.
  
  
  RESULTS
  The lymph node metastasis rates were 70% and 21% in the LVI-positive and LVI-negative groups, respectively. The nodal metastasis rate was higher in the LVI-positive group than in the LVI-negative group. Multivariate regression analysis showed that LVI was related to nodal metastasis (P<0.001). The median survival time of the patients was 26 and 43 months in the LVI-positive and LVI-negative groups, respectively. Although univariate regression analysis showed significant difference between the two groups (P=0.014), multivariate regression analysis revealed that LVI was not an independent prognostic factor for overall survival in the ESCC patients (P=0.062). Lymphatic node metastasis (P=0.031), clinical stage (P=0.019), and residual tumor (P=0.026) were the independent prognostic factors.
  
  
  CONCLUSIONS
  LVI labeled by D2-40 monoclonal antibody is a risk factor predictive of lymph node metastasis in ESCC patients.
authors.affiliation: Department of Radiation Oncology, Qilu Hospital of Shandong University, Jinan 250012, China; ; Department of Oncology, Yiyuan Chinese Medicine Hospital, Zibo 256100, China;
authors.forename: Bing
authors.initials: B
authors.lastname: Bai
authors.forename: Wei
authors.initials: W
authors.lastname: Ma
authors.forename: Kai
authors.initials: K
authors.lastname: Wang
authors.forename: Sita
authors.initials: S
authors.lastname: Ha
authors.forename: Jian-Bo
authors.initials: JB
authors.lastname: Wang
authors.forename: Bing-Xu
authors.initials: BX
authors.lastname: Tan
authors.forename: Na-Na
authors.initials: NN
authors.lastname: Wang
authors.forename: Sheng-Si
authors.initials: SS
authors.lastname: Yang
authors.forename: Yi-Bin
authors.initials: YB
authors.lastname: Jia
authors.forename: Yu-Feng
authors.initials: YF
authors.lastname: Cheng
keywords: D2-40; Esophageal squamous cell carcinoma; lymph node metastasis; lymphatic vessel invasion; prognosis