title: Promotion of wound healing using adipose-derived stem cells in radiation ulcer of a rat model.
abstract: BACKGROUND
  Wound healing is a complex biologic process that involves the integration of inflammation, mitosis, angiogenesis, synthesis, and remodeling of the extracellular matrix. However, some wounds fail to heal properly and become chronic. Although some simulated chronic wound models have been established, an efficient approach to treat chronic wounds in animal models has not been determined. The aim of this study was to develop a modified rat model simulating the chronic wounds caused by clinical radiation ulcers and examine the treatment of chronic wounds with adipose-derived stem cells.
  
  
  RESULTS
  Sprague-Dawley rats were irradiated with an electron beam, and wounds were created. The rats received treatment with adipose-derived stem cells (ASCs), and a wound-healing assay was performed. The wound sizes after ASC treatment for 3 weeks were significantly smaller compared with the control condition (p < 0.01). Histological observations of the wound edge and immunoblot analysis of the re-epithelialization region both indicated that the treatment with ASCs was associated with the development of new blood vessels. Cell-tracking experiments showed that ASCs were colocalized with endothelial cell markers in ulcerated tissues.
  
  
  CONCLUSIONS
  We established a modified rat model of radiation-induced wounds and demonstrated that ASCs accelerate wound-healing.
authors.forename: Sheng-Ping
authors.initials: SP
authors.lastname: Huang
authors.forename: Chun-Hsiang
authors.initials: CH
authors.lastname: Huang
authors.forename: Jia-Fwu
authors.initials: JF
authors.lastname: Shyu
authors.forename: Herng-Sheng
authors.initials: HS
authors.lastname: Lee
authors.forename: Shyi-Gen
authors.initials: SG
authors.lastname: Chen
authors.forename: James Yi-Hsin
authors.initials: JY
authors.lastname: Chan
authors.forename: Shih-Ming
authors.initials: SM
authors.lastname: Huang
mesh_terms: D000273:Adipose Tissue / Q000166:cytology; D000818:Animals; D005109:Extracellular Matrix / Q000473:pathology; D011878:Radiotherapy / Q000009:adverse effects; D051381:Rats; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000166:cytology; D014456:Ulcer / Q000473:pathology / Q000628:therapy*; D014945:Wound Healing