title: Antibiotic resistance and OXA-type carbapenemases-encoding genes in airborne Acinetobacter baumannii isolated from burn wards.
abstract: The study was conducted to investigate drug resistance, OXA-type carbapenemases-encoding genes and genetic diversity in airborne Acinetobacter baumannii (A. baumannii) in burn wards. Airborne A. baumannii were collected in burn wards and their corridors using Andersen 6-stage air sampler from January to June 2011. The isolates susceptibility to 13 commonly used antibiotics was examined according to the CLSI guidelines; OXA-type carbapenemases-encoding genes and molecular diversity of isolates were analyzed, respectively. A total of 16 non-repetitive A. baumannii were isolated, with 10 strains having a resistance rate of greater than 50% against the 13 antibiotics. The resistance rate against ceftriaxone, cyclophosvnamide, ciprofloxacin, and imipenem was 93.75% (15/16), but no isolate observed to be resistant to cefoperazone/sulbactam. Resistance gene analyses showed that all 16 isolates carried OXA-51, and 15 isolates carried OXA-23 except No.15; but OXA-24 and OXA-58 resistance genes not detected. The isolates were classified into 13 genotypes (A-M) according to repetitive extragenic palindromic sequence PCR (REP-PCR) results and only six isolates had a homology ≥90%. In conclusion, airborne A. baumannii in the burn wards had multidrug resistance and complex molecular diversity, and OXA-23 and OXA-51 were dominant mechanisms for resisting carbapenems.
authors.affiliation: College of Animal Science and Veterinary Medicine, Shandong Agricultural University, China, Sino-German Cooperative Research Centre for Zoonosis of Animal Origin Shandong Province, Tai'an Shandong, China; Tai'an City Central Hospital, Tai'an, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Gao
authors.affiliation: College of Animal Science and Veterinary Medicine, Shandong Agricultural University, China, Sino-German Cooperative Research Centre for Zoonosis of Animal Origin Shandong Province, Tai'an Shandong, China.
authors.forename: Xiaonan
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Tai'an City Central Hospital, Tai'an, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Bao
authors.affiliation: Affiliated Hospital of the Shandong Agricultural University, Tai'an, China.
authors.forename: Ruihua
authors.initials: R
authors.lastname: Ma
authors.affiliation: Daiyue Husbandry Bureau of Tai'an, Tai'an, China.
authors.forename: Yufa
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: College of Animal Science and Veterinary Medicine, Shandong Agricultural University, China, Sino-German Cooperative Research Centre for Zoonosis of Animal Origin Shandong Province, Tai'an Shandong, China; Tai'an City Central Hospital, Tai'an, China; Affiliated Hospital of the Shandong Agricultural University, Tai'an, China; Daiyue Husbandry Bureau of Tai'an, Tai'an, China.
authors.forename: Xinxian
authors.initials: X
authors.lastname: Li
authors.affiliation: College of Animal Science and Veterinary Medicine, Shandong Agricultural University, China, Sino-German Cooperative Research Centre for Zoonosis of Animal Origin Shandong Province, Tai'an Shandong, China. Electronic address: chaitj117@163.com.
authors.forename: Tongjie
authors.initials: T
authors.lastname: Chai
authors.affiliation: College of Animal Science and Veterinary Medicine, Shandong Agricultural University, China, Sino-German Cooperative Research Centre for Zoonosis of Animal Origin Shandong Province, Tai'an Shandong, China. Electronic address: caiyum@163.com.
authors.forename: Yumei
authors.initials: Y
authors.lastname: Cai
mesh_terms: D040981:Acinetobacter baumannii / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D000391:Air Microbiology; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000235:genetics; D002053:Burn Units; D015780:Carbapenems / Q000494:pharmacology*; D002438:Cefoperazone / Q000494:pharmacology; D002443:Ceftriaxone / Q000494:pharmacology; D002939:Ciprofloxacin / Q000494:pharmacology; D004269:DNA, Bacterial / Q000235:genetics*; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics; D006801:Humans; D015378:Imipenem / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D016133:Polymerase Chain Reaction; D012091:Repetitive Sequences, Nucleic Acid; D013407:Sulbactam / Q000494:pharmacology; D001618:beta-Lactamases / Q000235:genetics*
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D015780:Carbapenems; D004269:DNA, Bacterial; D002939:Ciprofloxacin; D015378:Imipenem; D002443:Ceftriaxone; D002438:Cefoperazone; C466679:beta-lactamase OXA-23; D001618:beta-Lactamases; C410345:beta-lactamase OXA-24; C577373:beta-lactamase OXA-51, Acinetobacter baumannii; C534889:beta-lactamase OXA-58, Acinetobacter baumannii; D013407:Sulbactam
keywords: Airborne Acinetobacter baumannii; Burn wards; Molecular diversity; OXA-type carbapenemases-encoding resistance genes; REP-PCR