title: Herpesvirus 6 glycoproteins B (gB), gH, gL, and gQ are necessary and sufficient for cell-to-cell fusion.
abstract: The human herpesvirus 6 (HHV-6) envelope glycoprotein gH/gL/gQ1/gQ2 complex associates with host cell CD46 as its cellular receptor. Although gB has been suggested to be involved in HHV-6 infection, its function in membrane fusion has remained unclear. Here, we have developed an HHV-6A (strain GS)and HHV-6B (strain Z29) virus-free cell-to-cell fusion assay and demonstrate that gB and the gH/gL/gQ1/gQ2 complex are the minimum components required for membrane fusion by HHV-6.
authors.affiliation: Department of Immunochemistry, Research Institute for Microbial Diseases, Osaka University, Osaka, Japan.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Tanaka
authors.forename: Tadahiro
authors.initials: T
authors.lastname: Suenaga
authors.forename: Misako
authors.initials: M
authors.lastname: Matsumoto
authors.forename: Tsukasa
authors.initials: T
authors.lastname: Seya
authors.forename: Hisashi
authors.initials: H
authors.lastname: Arase
mesh_terms: D002459:Cell Fusion; D002478:Cells, Cultured; D004279:DNA, Viral / Q000235:genetics; D005434:Flow Cytometry; D006023:Glycoproteins / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D006566:Herpesviridae Infections / Q000235:genetics / Q000378:metabolism* / Q000821:virology; D015654:Herpesvirus 6, Human / Q000502:physiology*; D006801:Humans; D007668:Kidney / Q000166:cytology / Q000378:metabolism / Q000821:virology; D008156:Luciferases / Q000378:metabolism; D051916:Membrane Cofactor Protein / Q000235:genetics / Q000378:metabolism*; D008561:Membrane Fusion; D016133:Polymerase Chain Reaction
chemical_list: D004279:DNA, Viral; D006023:Glycoproteins; D051916:Membrane Cofactor Protein; D008156:Luciferases