title: Short-term administration of alibernet red wine extract failed to affect blood pressure and to improve endothelial function in young normotensive and spontaneously hypertensive rats.
abstract: As wine polyphenols were shown to possess many positive effects in mammals, including improvement of vascular function, this study investigated the effect of the Slovak Alibernet red wine extract (AWE) on blood pressure and vascular function in young normotensive Wistar-Kyoto (WKY) and spontaneously hypertensive (SHR) rats. Six weeks old, male, WKY and SHR were treated with AWE for three weeks at the dose of 24.2 mg/kg/day. Blood pressure (BP), determined by tail-cuff plethysmography, was significantly elevated in SHR vs. WKY and AWE failed to affect it. Lipid peroxidation was evaluated by determination of thiobarbituric acid-reactive substances. Vascular function was assessed in rings of the femoral artery using Mulvany-Halpern's myograph. Maximal endothelium-dependent acetylcholine (ACh)-induced relaxation was reduced in control SHR vs. WKY rats by approximately 9.3 %, which was associated with a significant decrease of its NO-independent component. AWE failed to affect maximal ACh-induced relaxation, both its NO-dependent and independent components, compared to controls of the same genotype. AWE however reduced lipid peroxidation in the left ventricle of both WKY and SHR and in the liver of SHR. In conclusion, three-week administration of AWE failed to reduce BP and to improve endothelial function in the femoral arteries of both genotypes investigated.
authors.affiliation: Institute of Normal and Pathological Physiology, Slovak Academy of Sciences, Bratislava, Slovak Republic. Iveta.Bernatova@savba.sk.
authors.forename: P
authors.initials: P
authors.lastname: Bališ
authors.forename: A
authors.initials: A
authors.lastname: Púzserová
authors.forename: P
authors.initials: P
authors.lastname: Slezák
authors.forename: N
authors.initials: N
authors.lastname: Sestáková
authors.forename: O
authors.initials: O
authors.lastname: Pecháňová
authors.forename: I
authors.initials: I
authors.lastname: Bernátová
mesh_terms: D000818:Animals; D001794:Blood Pressure / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004730:Endothelium, Vascular / Q000187:drug effects / Q000503:physiopathology*; D006973:Hypertension / Q000188:drug therapy* / Q000209:etiology / Q000503:physiopathology*; D008297:Male; D058729:Peripheral Arterial Disease / Q000150:complications / Q000188:drug therapy* / Q000503:physiopathology*; D010936:Plant Extracts / Q000008:administration & dosage*; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY; D000077185:Resveratrol; D013267:Stilbenes / Q000008:administration & dosage*; D017211:Treatment Failure; D016896:Treatment Outcome; D014655:Vascular Resistance / Q000187:drug effects; D014920:Wine
chemical_list: D010936:Plant Extracts; D013267:Stilbenes; D000077185:Resveratrol