title: Capillary zone electrophoresis with on-line blotting for separation and detection of (32)P-postlabelled DNA adducts.
abstract: A new technique for the detection of (32)P-postlabelled DNA adducts separated by capillary electrophoresis was developed. By direct transfer from the capillary outlet to a positively charged moving filter paper, eluted radioactive peaks can be quantified using a phosphor imaging detector. With this method it is possible to separate DNA adducts from different carcinogens after (32)P-postlabelling of the modified and unmodified nucleotides with high sensitivity approaching 1 adduct per 10(9) nucleotides.
authors.affiliation: Walther Straub-Institut für Pharmakologie und Toxikologie, Nussbaumstr. 26, D-80336 München, Germany.
authors.forename: O S
authors.initials: OS
authors.lastname: Richter