title: HPLC analysis of phenolics compounds and antioxidant capacity of leaves of Vitex megapotamica (Sprengel) Moldenke.
abstract: Vitex megapotamica (Sprengel) Moldenke belongs to the Verbenaceae family and is popularly known as "tarumã". The antioxidant capacity of fractions and crude extract from the leaves of V. megapotamica were determined in this study through the capacity to remove reactive species and phenolic compounds were quantified in the various fractions. The IC50 (DPPH) ranged from 14.17 ± 0.76 to 37.63 ± 0.98 µg/mL. The ethyl acetate fraction might contain the strongest lipid peroxidation inhibitory compounds with an IC50 of 16.36 ± 5.09 µg/mL, being also the one with the highest content of polyphenols (522.4 ± 1.12 mg/g), flavonoids (220.48 ± 0.30 mg/g) and condensed tannins (3.86 ± 0.53 mg/g). Compounds quantified by HPLC/DAD in the crude extract and fractions were chlorogenic and rosmarinic acids. Higher dosages of the extracts were more effective in reducing levels of plasma protein carbonyls and were also shown to be able to remove reactive species by a 2',7'-dichlorofluorescein diacetate assay, reducing oxidative stress in all tested fractions. Results obtained indicated that V. megapotamica exhibits good potential to prevent diseases caused by the overproduction of free radicals and it might also be used as a potential source of natural antioxidant agents.
authors.affiliation: Post-Graduate Program in Pharmaceutical Sciences, Federal University of Santa Maria, Camobi Campus, Santa Maria, RS, 97105-900, Brazil.
authors.forename: Thiele Faccim
authors.initials: TF
authors.lastname: de Brum
authors.forename: Marina
authors.initials: M
authors.lastname: Zadra
authors.forename: Mariana
authors.initials: M
authors.lastname: Piana
authors.forename: Aline Augusti
authors.initials: AA
authors.lastname: Boligon
authors.forename: Janaina Kieling
authors.initials: JK
authors.lastname: Fröhlich
authors.forename: Robson Borba
authors.initials: RB
authors.lastname: de Freitas
authors.forename: Sílvio Terra
authors.initials: ST
authors.lastname: Stefanello
authors.forename: Amanda Luana Forbrig
authors.initials: AL
authors.lastname: Froeder
authors.forename: Bianca Vargas
authors.initials: BV
authors.lastname: Belke
authors.forename: Letícia Teixeira
authors.initials: LT
authors.lastname: Nunes
authors.forename: Roberta
authors.initials: R
authors.lastname: da Silva Jesus
authors.forename: Michel Mansur
authors.initials: MM
authors.lastname: Machado
authors.forename: João Batista Teixeira
authors.initials: JB
authors.lastname: da Rocha
authors.forename: Félix Alexandre Antunes
authors.initials: FA
authors.lastname: Soares
authors.forename: Margareth Linde
authors.initials: ML
authors.lastname: Athayde
mesh_terms: D000975:Antioxidants / Q000032:analysis* / Q000737:chemistry / Q000494:pharmacology; D002726:Chlorogenic Acid / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D002934:Cinnamates / Q000032:analysis; D053630:Depsides / Q000032:analysis; D005419:Flavonoids / Q000032:analysis* / Q000737:chemistry / Q000494:pharmacology; D010936:Plant Extracts / Q000737:chemistry / Q000493:pharmacokinetics; D018515:Plant Leaves / Q000737:chemistry; D059808:Polyphenols / Q000032:analysis* / Q000737:chemistry / Q000494:pharmacology; D017382:Reactive Oxygen Species / Q000378:metabolism; D013634:Tannins / Q000032:analysis* / Q000737:chemistry / Q000494:pharmacology; D020950:Vitex / Q000737:chemistry*; D000097123:Rosmarinic Acid
chemical_list: D000975:Antioxidants; D002726:Chlorogenic Acid; D002934:Cinnamates; D053630:Depsides; D005419:Flavonoids; D010936:Plant Extracts; D059808:Polyphenols; D017382:Reactive Oxygen Species; D013634:Tannins; D000097123:Rosmarinic Acid