title: In vitro skin permeation and anti-atopic efficacy of lipid nanocarriers containing water soluble extracts of Houttuynia cordata.
abstract: OBJECTIVE
  The aims of this work are to enhance the in vitro skin permeation of Houttuynia cordata (water-soluble extract of H. cordata; HCWSE) and to boost the efficacy of HCWSE against atopic dermatitis (AD) - like skin lesion in hairless mice using lipid nano-carriers (liposome and cubosome).
  
  
  METHODS
  HCWSE was obtained by a hot water extraction. Monoolein cubosomal suspension containing HCWSE and egg phosphatidylcholine liposomal suspension containing the same was prepared by a sonication and a film hydration method, respectively.
  
  
  RESULTS
  The lipid nano-carriers, especially cubosome, enhanced the in vitro skin permeation of HCWSE. The inhibitory effects of HCWSE-containing lipid carrier suspensions on the development of 1-chloro-2,4-dinitrobenzene (DNCB)-induced AD-like skin lesion in hairless mice were investigated by observing appearance of skin surface, serum immunoglobulin E (IgE) level and cytokine expression. HCWSE-containing preparations suppressed IgE production and interleukin 4 expression, whereas they promoted interferon gamma expression. The order of lymphocyte (B-cell, Th1 cell and Th2 cell) modulating effect was HCWSE-containing cubosomal suspension > HCWSE-containing liposomal suspension > HCWSE solution in phosphate buffered saline, indicating that the cubosomal suspension, among the preparations, was the most efficacious in inhibiting the development of DNCB-induced AD-like skin lesion.
  
  
  CONCLUSIONS
  It is believed that the cubosomal suspension containing HCWSE would be an efficacious preparation for the treatment of AD.
authors.affiliation: Division of Biotechnology & Bioengineering, Institute of Bioscience and Biotechnology, Kangwon National University , Chunchon , Republic of Korea.
authors.forename: Taek Kwan
authors.initials: TK
authors.lastname: Kwon
authors.forename: Jin-Chul
authors.initials: JC
authors.lastname: Kim
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000378:metabolism; D016207:Cytokines / Q000378:metabolism; D003876:Dermatitis, Atopic / Q000188:drug therapy* / Q000473:pathology; D004137:Dinitrochlorobenzene / Q000633:toxicity; D004195:Disease Models, Animal; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage* / Q000493:pharmacokinetics / Q000494:pharmacology; D005260:Female; D052859:Houttuynia; D007073:Immunoglobulin E / Q000097:blood; D008055:Lipids / Q000737:chemistry; D008081:Liposomes; D051379:Mice; D008812:Mice, Hairless; D053758:Nanoparticles; D012869:Skin Absorption; D018417:Th1 Cells / Q000378:metabolism; D018418:Th2 Cells / Q000378:metabolism; D014867:Water / Q000737:chemistry
chemical_list: D016207:Cytokines; D004137:Dinitrochlorobenzene; D004365:Drugs, Chinese Herbal; C442781:Houttuynia cordata extract; D008055:Lipids; D008081:Liposomes; D014867:Water; D007073:Immunoglobulin E
keywords: Atopic dermatitis; Houttuynia cordata; cubosome; cytokines; immunoglobulin E; liposome