title: In-situ transmission electron microscopy of liposomes in an aqueous environment.
abstract: The characterization of liposomes was undertaken using in-situ microfluidic transmission electron microscopy. Liposomes were imaged without contrast enhancement staining or cryogenic treatment, allowing for the observation of functional liposomes in an aqueous environment. The stability and quality of the liposome structures observed were found to be highly dependent on the surface and liposome chemistries within the liquid cell. The successful imaging of liposomes suggests the potential for the extension of in-situ microfluidic TEM to a wide variety of other biological and soft matter systems and processes.
authors.affiliation: Sandia National Laboratories, Albuquerque, New Mexico 87185, United States.
authors.forename: Sarah M
authors.initials: SM
authors.lastname: Hoppe
authors.forename: Darryl Y
authors.initials: DY
authors.lastname: Sasaki
authors.forename: Aubrianna N
authors.initials: AN
authors.lastname: Kinghorn
authors.forename: Khalid
authors.initials: K
authors.lastname: Hattar
mesh_terms: D008081:Liposomes / Q000737:chemistry*; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation; D046529:Microscopy, Electron, Transmission / Q000295:instrumentation; D010316:Particle Size; D013499:Surface Properties; D014867:Water / Q000737:chemistry
chemical_list: D008081:Liposomes; D014867:Water