title: Modifying bone scaffold architecture in vivo with permanent magnets to facilitate fixation of magnetic scaffolds.
abstract: The fundamental elements of tissue regeneration are cells, biochemical signals and the three-dimensional microenvironment. In the described approach, biomineralized-collagen biomaterial functions as a scaffold and provides biochemical stimuli for tissue regeneration. In addition superparamagnetic nanoparticles were used to magnetize the biomaterials with direct nucleation on collagen fibres or impregnation techniques. Minimally invasive surgery was performed on 12 rabbits to implant cylindrical NdFeB magnets in close proximity to magnetic scaffolds within the lateral condyles of the distal femoral epiphyses. Under this static magnetic field we demonstrated, for the first time in vivo, that the ability to modify the scaffold architecture could influence tissue regeneration obtaining a well-ordered tissue. Moreover, the association between NdFeB magnet and magnetic scaffolds represents a potential technique to ensure scaffold fixation avoiding micromotion at the tissue/biomaterial interface.
authors.affiliation: Laboratory of Biomechanics and Technology Innovation, Rizzoli Orthopaedic Institute, Bologna, Italy. s.panseri@biomec.ior.it
authors.forename: S
authors.initials: S
authors.lastname: Panseri
authors.forename: A
authors.initials: A
authors.lastname: Russo
authors.forename: M
authors.initials: M
authors.lastname: Sartori
authors.forename: G
authors.initials: G
authors.lastname: Giavaresi
authors.forename: M
authors.initials: M
authors.lastname: Sandri
authors.forename: M
authors.initials: M
authors.lastname: Fini
authors.forename: M C
authors.initials: MC
authors.lastname: Maltarello
authors.forename: T
authors.initials: T
authors.lastname: Shelyakova
authors.forename: A
authors.initials: A
authors.lastname: Ortolani
authors.forename: A
authors.initials: A
authors.lastname: Visani
authors.forename: V
authors.initials: V
authors.lastname: Dediu
authors.forename: A
authors.initials: A
authors.lastname: Tampieri
authors.forename: M
authors.initials: M
authors.lastname: Marcacci
mesh_terms: D000818:Animals; D008280:Magnetics; D008297:Male; D011817:Rabbits; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds
keywords: Bone; Fixation; Magnetic field; Oriented architecture; Scaffold