title: In vitro CPC retention and VSC adsorption by IPM oil droplets: possible mechanisms of action of a two phase mouthwash.
abstract: Two phase oil-water mouthwash has been previously shown to efficiently bind oral microorganisms, relying on their cell surface hydrophobicity. The aim of the present in vitro study was to test the cetylpyridinium chloride (CPC) retention and volatile sulfide compounds (VSCs) adsorption abilities of the oil droplets created by mixing of a two phase oil-water solution. VSC adsorption was assayed using a salivary incubation assay and garlic powder solutions, and demonstrated using microscopic sulfide assay. CPC retention was assayed by kinetic and endpoint measurement of Streptococcus salivarius outgrowth using microplate (ELISA) reader. Results showed that the isopropyl myristate (IPM) oil droplets in the two phase solutions were able to adsorb 68-80% of VSCs. CPC at a concentration of 0.05% was most affectively retained by the oil droplets showing a significantly increase in residual antibacterial activity against Streptococcus salivarius. These results taken together, suggests that VSC adsorption and CPC retention by IPM oil droplets may be two additional mechanisms in the activity of the two phase mouthwash formulation.
authors.affiliation: Department of Prosthodontics, Goldschleger School of Dental Medicine, Tel-Aviv University, Ramat-Aviv 69978, Israel. drsterer@gmail.com
authors.forename: N
authors.initials: N
authors.lastname: Sterer
authors.forename: H
authors.initials: H
authors.lastname: Slutzky
authors.forename: D
authors.initials: D
authors.lastname: Kohavi
authors.forename: S
authors.initials: S
authors.lastname: Matalon
mesh_terms: D000327:Adsorption; D000891:Anti-Infective Agents, Local / Q000493:pharmacokinetics; D001944:Breath Tests; D002594:Cetylpyridinium / Q000493:pharmacokinetics*; D006801:Humans; D009067:Mouthwashes / Q000493:pharmacokinetics*; D009226:Myristates / Q000493:pharmacokinetics*; D012463:Saliva / Q000378:metabolism*; D012969:Sodium Fluoride / Q000493:pharmacokinetics; D013440:Sulfides / Q000493:pharmacokinetics
chemical_list: D000891:Anti-Infective Agents, Local; D009067:Mouthwashes; D009226:Myristates; D013440:Sulfides; C008205:isopropyl myristate; D012969:Sodium Fluoride; D002594:Cetylpyridinium