title: Monoester-Diterpene Aconitum Alkaloid Metabolism in Human Liver Microsomes: Predominant Role of CYP3A4 and CYP3A5.
abstract: Aconitum, widely used to treat rheumatoid arthritis for thousands of years, is a toxic herb that can frequently cause fatal cardiac poisoning. Aconitum toxicity could be decreased by properly hydrolyzing diester-diterpene alkaloids into monoester-diterpene alkaloids. Monoester-diterpene alkaloids, including benzoylaconine (BAC), benzoylmesaconine (BMA), and benzoylhypaconine (BHA), are the primary active and toxic constituents of processed Aconitum. Cytochrome P450 (CYP) enzymes protect the human body by functioning as the defense line that limits the invasion of toxicants. Our purposes were to identify the CYP metabolites of BAC, BMA, and BHA in human liver microsomes and to distinguish which isozymes are responsible for their metabolism through the use of chemical inhibitors, monoclonal antibodies, and cDNA-expressed CYP enzyme. High-resolution mass spectrometry was used to characterize the metabolites. A total of 7, 8, and 9 metabolites were detected for BAC, BMA, and BHA, respectively. The main metabolic pathways were demethylation, dehydrogenation, demethylation-dehydrogenation, hydroxylation and didemethylation, which produced less toxic metabolites by decomposing the group responsible for the toxicity of the parent compound. Taken together, the results of the chemical inhibitors, monoclonal antibodies, and cDNA-expressed CYP enzymes experiments demonstrated that CYP3A4 and CYP3A5 have essential functions in the metabolism of BAC, BMA, and BHA.
authors.affiliation: Department of Pharmaceutics, School of Pharmaceutical Sciences, Southern Medical University, Guangzhou, Guangdong 510515, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Ye
authors.forename: Xiao-Shan
authors.initials: XS
authors.lastname: Yang
authors.forename: Lin-Lin
authors.initials: LL
authors.lastname: Lu
authors.forename: Wei-Ying
authors.initials: WY
authors.lastname: Chen
authors.forename: Shan
authors.initials: S
authors.lastname: Zeng
authors.forename: Tong-Meng
authors.initials: TM
authors.lastname: Yan
authors.forename: Ling-Na
authors.initials: LN
authors.lastname: Dong
authors.forename: Xiao-Juan
authors.initials: XJ
authors.lastname: Peng
authors.forename: Jian
authors.initials: J
authors.lastname: Shi
authors.forename: Zhong-Qiu
authors.initials: ZQ
authors.lastname: Liu