title: Detection of the enterotoxigenic genes (sei,sej) inStaphylococcus aureusisolates from bovine mastitis milk in the West Azerbaijan of Iran.
abstract: Staphylococcus aureusis a major causative pathogen of clinical and subclinical mastitis of dairy domestic ruminants. This organism produces a variety of extracellular toxins and virulence factors such as enterotoxin SEI and SEJ that contribute to its pathogenic potential. In this study 25S. aureusisolates obtained from four dairy herds of Urmia region which is located in West Azerbaijan province in Iran. The tested isolates were identified on the basis of the cultural and biochemical properties, as well as amplification of thearoAgene which is specific forS. aureus. All isolates were also analyzed for the presence of the SEI (sei) and SEJ (sej) encoding genes using polymerase chain reaction (PCR). Seven positive isolates were detected forsei, butsejgene was not detected in any of the total number of 25 isolates. The present study revealed that the PCR amplification of thearoAgene could be used as a powerful tool for identification ofS. aureusfrom the cases of bovine mastitis. Results of the present study also showed that the strains ofS. aureuswhich cause mastitis can potentially produce enterotoxin SEI. Overall, our results suggest that it is of special importance to follow the presence of enterotoxin-producingS. aureusin other dairy products, especially for protecting the consumers from staphylococcal food poisoning.
authors.affiliation: Department of Microbiology, Faculty of Veterinary Medicine, Urmia University, P.O. Box 1177, Urmia, Iran.
authors.forename: Malahat
authors.initials: M
authors.lastname: Ahmady
authors.forename: Sahar
authors.initials: S
authors.lastname: Kazemi
keywords: Bovine mastitis; Enterotoxin; PCR; S. aureus