title: An analytical approach based on ESI-MS, LC-MS and PCA for the quali-quantitative analysis of cycloartane derivatives in Astragalus spp.
abstract: Astragalus species are widely used as health foods and dietary supplements, as well as drugs in traditional medicine. To rapidly evaluate metabolite similarities and differences among the EtOH extracts of the roots of eight commercial Astragalus spp., an approach based on direct analyses by ESI-MS followed by PCA of ESI-MS data, was carried out. Successively, quali-quantitative analyses of cycloartane derivatives in the eight Astragalus spp. by LC-ESI-MS(n) and PCA of LC-ESI-MS data were performed. This approach allowed to promptly highlighting metabolite similarities and differences among the various Astragalus spp. PCA results from LC-ESI-MS data of Astragalus samples were in reasonable agreement with both PCA results of ESI-MS data and quantitative results. This study affords an analytical method for the quali-quantitative determination of cycloartane derivatives in herbal preparations used as health and food supplements.
authors.affiliation: Dipartimento di Farmacia, Università degli Studi di Salerno, Via Giovanni Paolo II, n. 132, 84084 Fisciano, SA, Italy.
authors.forename: Assunta
authors.initials: A
authors.lastname: Napolitano
authors.forename: Seref
authors.initials: S
authors.lastname: Akay
authors.forename: Angela
authors.initials: A
authors.lastname: Mari
authors.forename: Erdal
authors.initials: E
authors.lastname: Bedir
authors.forename: Cosimo
authors.initials: C
authors.lastname: Pizza
authors.forename: Sonia
authors.initials: S
authors.lastname: Piacente
mesh_terms: D027883:Astragalus Plant / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D010936:Plant Extracts / Q000032:analysis; D025341:Principal Component Analysis; D021241:Spectrometry, Mass, Electrospray Ionization; D014315:Triterpenes / Q000032:analysis*
chemical_list: D010936:Plant Extracts; D014315:Triterpenes; C085352:cycloartane
keywords: Astragalus genus; Cycloartanes; ESI-MS; LC-ESI-MS; PCA