title: A TAF4 coactivator function for E proteins that involves enhanced TFIID binding.
abstract: The multisubunit TFIID plays a direct role in transcription initiation by binding to core promoter elements and directing preinitiation complex assembly. Although TFIID may also function as a coactivator through direct interactions with promoter-bound activators, mechanistic aspects of this poorly defined function remain unclear. Here, biochemical studies show a direct TFIID-E-protein interaction that (1) is mediated through interaction of a novel E-protein activation domain (activation domain 3 [AD3]) with the TAF homology (TAFH) domain of TAF4, (2) is critical for activation of a natural target gene by an E protein, and (3) mechanistically acts by enhancing TFIID binding to the core promoter. Complementary assays establish a gene-specific role for the TAFH domain in TFIID recruitment and activation of a large subset of genes in vivo. These results firmly establish TAF4 as a bona fide E-protein coactivator as well as a mechanism involving facilitated TFIID binding through direct interaction with an E-protein activation domain.
authors.affiliation: Laboratory of Biochemistry and Molecular Biology, The Rockefeller University, New York, New York 10065, USA.
authors.forename: Wei-Yi
authors.initials: WY
authors.lastname: Chen
authors.forename: Jinsong
authors.initials: J
authors.lastname: Zhang
authors.forename: Huimin
authors.initials: H
authors.lastname: Geng
authors.forename: Zhimei
authors.initials: Z
authors.lastname: Du
authors.forename: Tomoyoshi
authors.initials: T
authors.lastname: Nakadai
authors.forename: Robert G
authors.initials: RG
authors.lastname: Roeder
mesh_terms: D051792:Basic Helix-Loop-Helix Proteins / Q000235:genetics / Q000378:metabolism*; D017353:Gene Deletion; D005786:Gene Expression Regulation; D006367:HeLa Cells; D006801:Humans; D011401:Promoter Regions, Genetic / Q000235:genetics; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D035381:TATA-Binding Protein Associated Factors / Q000378:metabolism*; D035362:Transcription Factor TFIID / Q000235:genetics* / Q000378:metabolism*; D059467:Transcriptome
chemical_list: D051792:Basic Helix-Loop-Helix Proteins; D035381:TATA-Binding Protein Associated Factors; D035362:Transcription Factor TFIID; C101882:TAF4 protein, human
keywords: E proteins; E2A; HEB; TAF4; TAFH; TFIID; transcription coactivator