title: Structural peculiarities of the (MHF1-MHF2)4 octamer provide a long DNA binding patch to anchor the MHF-FANCM complex to chromatin: a solution SAXS study.
abstract: MHF1 and MHF2 are histone-fold-containing FANCM-associated proteins. FANCM is a Fanconi anemia (FA) complementation group protein. We previously obtained high-resolution structures of MHF1-MHF2 (MHF) and MHF in complex with a fragment of FANCM (MHF-FANCM-F). Here, we use small angle X-ray scattering (SAXS) to investigate the solution behaviors of these protein complexes. In combination with crystallographic data, the results of the SAXS study reveal that a long, positively charged patch exposed on the surface of the MHF complex plays a critical role in double-stranded DNA (dsDNA) binding.
authors.affiliation: Beijing Synchrotron Radiation Facility, Institute of High Energy Physics, Chinese Academy of Sciences, Beijing 100049, China.
authors.forename: Wenjia
authors.initials: W
authors.lastname: Wang
authors.forename: Qiong
authors.initials: Q
authors.lastname: Guo
authors.forename: Eleonora V
authors.initials: EV
authors.lastname: Shtykova
authors.forename: Guangfeng
authors.initials: G
authors.lastname: Liu
authors.forename: Jianhua
authors.initials: J
authors.lastname: Xu
authors.forename: Maikun
authors.initials: M
authors.lastname: Teng
authors.forename: Peng
authors.initials: P
authors.lastname: Liu
authors.forename: Yuhui
authors.initials: Y
authors.lastname: Dong
mesh_terms: D051017:Apoptosis Regulatory Proteins / Q000737:chemistry* / Q000235:genetics; D001665:Binding Sites; D002843:Chromatin / Q000737:chemistry* / Q000235:genetics; D004247:DNA / Q000737:chemistry* / Q000235:genetics; D004265:DNA Helicases / Q000737:chemistry* / Q000235:genetics; D004268:DNA-Binding Proteins / Q000737:chemistry* / Q000235:genetics; D015870:Gene Expression; D006801:Humans; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000737:chemistry* / Q000235:genetics; D011485:Protein Binding; D055503:Protein Multimerization; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics; D053838:Scattering, Small Angle; D012996:Solutions; D055672:Static Electricity; D025521:Tumor Suppressor Proteins / Q000737:chemistry* / Q000235:genetics; D014961:X-Ray Diffraction
chemical_list: D051017:Apoptosis Regulatory Proteins; C493243:CENPS protein, human; C551977:CENPX protein, human; D002843:Chromatin; D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D011994:Recombinant Proteins; D012996:Solutions; D025521:Tumor Suppressor Proteins; D004247:DNA; C518295:FANCM protein, human; D004265:DNA Helicases
keywords: DNA binding patch; FA; FANCM; Fanconi anaemia; Fanconi anemia; ICLs; MHF; MHF complex; MHF in complex with a fragment of FANCM; MHF1–MHF2; MHF–FANCM-F; MM; SAXS; SEC; double strand DNA; dsDNA; interstrand crosslinks; molecular mass; size-exclusion chromatography