title: Stage-specific transcription during development of Aedes aegypti.
abstract: BACKGROUND
  Aedes aegypti is the most important global vector of dengue virus infection in humans. Availability of the draft genome sequence of this mosquito provides unique opportunities to study different aspects of its biology, including identification of genes and pathways relevant to the developmental processes associated with transition across individual life stages. However, detailed knowledge of gene expression patterns pertaining to developmental stages of A. aegypti is largely lacking.
  
  
  RESULTS
  We performed custom cDNA microarray analyses to examine the expression patterns among six developmental stages: early larvae, late larvae, early pupae, late pupae, and adult male and female mosquitoes. Results revealed 1,551 differentially expressed transcripts (DETs) showing significant differences in levels of expression between these life stages. The data suggests that most of the differential expression occurs in a stage specific manner in A. aegypti. Based on hierarchical clustering of expression levels, correlated expression patterns of DETs were also observed among developmental stages. Weighted gene correlation network analysis revealed modular patterns of expression among the DETs. We observed that hydrolase activity, membrane, integral to membrane, DNA binding, translation, ribosome, nucleoside-triphosphatase activity, structural constituent of ribosome, ribonucleoprotein complex and receptor activity were among the top ten ranked GO (Gene Ontology) terms associated with DETs. Significant associations of DETs were also observed with specific KEGG (Kyoto Encyclopedia of Genes and Genomes) pathway modules. Finally, comparisons with the previously reported developmental transcriptome of the malaria vector, Anopheles gambiae, indicated that gene expression patterns during developmental processes reflect both species-specific as well as common components of the two mosquito species.
  
  
  CONCLUSIONS
  Our study shows that genes involved in the developmental life cycle of A. aegypti are expressed in a highly stage-specific manner. This suggests that transcriptional events associated with transition through larval, pupal and adult stages are largely discrete.
authors.affiliation: Department of Biological Sciences, Eck Institute for Global Health, University of Notre Dame, Notre Dame, IN 46556, USA.
authors.forename: Brent W
authors.initials: BW
authors.lastname: Harker
authors.forename: Susanta K
authors.initials: SK
authors.lastname: Behura
authors.forename: Becky S
authors.initials: BS
authors.lastname: deBruyn
authors.forename: Diane D
authors.initials: DD
authors.lastname: Lovin
authors.forename: Akio
authors.initials: A
authors.lastname: Mori
authors.forename: Jeanne
authors.initials: J
authors.lastname: Romero-Severson
authors.forename: David W
authors.initials: DW
authors.lastname: Severson
mesh_terms: D000330:Aedes / Q000235:genetics / Q000254:growth & development*; D000818:Animals; D005260:Female; D015870:Gene Expression; D007814:Larva / Q000378:metabolism; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis; D014158:Transcription, Genetic