title: Detection of antibody to Purpureocillium lilacinum by immunofluorescent assay and flow cytometry in serum of infected C57BL/6 mice.
abstract: Purpureocillium lilacinum is an emerging pathogenic fungus that can cause different clinical manifestations ranging from cutaneous and sub-cutaneous infections to severe oculomycosis. In this study, using both conventional indirect immunofluorescence and non-conventional flow cytometry approaches, IgG antibodies were readily detected in both C57BL/6 immunocompetent and immunosuppressed mice after i.v. infection with P. lilacinum. The humoral immune response was specific, since virtually no antibodies were detected in the serum of control mice. Flow cytometry assays also showed both quantitative and qualitative differences in total IgG and its isotypes in sera of immunocompetent and immunosupressed infected mice. Although a good starting point, it is clear that the effectiveness of serological assays for P. lilacinum hyalohyphomycosis identification in clinical studies still requires further standardization. Upon further validation in humans, these techniques have the potential to be suitable to detect P. lilacinum infection in patients, thereby avoiding current laborious and time-consuming culture techniques.
authors.affiliation: Laboratório de Taxonomia, Bioquímica e Bioprospecção de Fungos, Instituto Oswaldo Cruz, FIOCRUZ, Rio de Janeiro, Brazil; Laboratório de Imunologia Clínica, Instituto Oswaldo Cruz, FIOCRUZ, Rio de Janeiro, Brazil.
authors.forename: Danielly C M
authors.initials: DC
authors.lastname: de Sequeira
authors.forename: Mariana L P
authors.initials: ML
authors.lastname: Peixoto
authors.forename: Paula M
authors.initials: PM
authors.lastname: De Luca
authors.forename: Joseli
authors.initials: J
authors.lastname: Oliveira-Ferreira
authors.forename: Paulo R Z
authors.initials: PR
authors.lastname: Antas
authors.forename: Cintia M
authors.initials: CM
authors.lastname: Borba
mesh_terms: D000818:Animals; D000908:Antibodies, Fungal / Q000097:blood*; D001203:Ascomycota / Q000276:immunology*; D003881:Dermatomycoses / Q000175:diagnosis* / Q000382:microbiology; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D060605:Hyalohyphomycosis / Q000175:diagnosis* / Q000382:microbiology; D007074:Immunoglobulin G / Q000097:blood*; D007165:Immunosuppression Therapy; D051379:Mice; D008810:Mice, Inbred C57BL
chemical_list: D000908:Antibodies, Fungal; D007074:Immunoglobulin G
keywords: Antibodies; Flow cytometry; Indirect immunofluorescence; Purpureocillium lilacinum