title: Histopathological and clinical evaluation of Kombucha tea and Nitrofurazone on cutaneous full-thickness wounds healing in rats: an experimental study.
abstract: BACKGROUND
  Kombucha, a fermented tea (KT) is claimed to possess many beneficial properties. The aim of this study was to evaluate clinical and histopathological alterations of Kombucha tea and Nitrofurazone on cutaneous full-thickness wounds healing in rat.
  
  
  METHODS
  In present study 24 Wister -albino rats weighing 150-200 g were selected and divided to two treatment groups as Nitrofurazone ointment (0.2%) and Kombucha tea. Subsequently, the anesthesia was exerted by Ketamin hydrochloride 10% (40 mg/kg) and Xylasine (2 mg/kg) through intra muscular (IM) route. Furthermore, upon preparation of dorsal region of the animal for surgery, a piece of full-thickness skin removed (2 × 2 cm). In order to comparing wounds healing clinically and histologically, once every four days from the commencement, the wounds were photographed and the healed surface was measured by Scion image software.
  
  
  RESULTS
  The clinical findings indicated that the Kombucha fungus resulted in precipitating healing than Nitrofurazone; however, it was not significant (p > 0.05). In order to pathological comparing of wound healing process, several wound biopsies were taken on 4, 8, 12, 16 and 20th days. Additionally, the histopathological results demonstrated that there was inflammation in Nitrofurazone group through twelveth day, somehow the epithelium was formed and abundant vessels were visible. Although on 16th day and the previous days the healing condition of Kombucha fungus was considered as minimal rate, revealing it is similar to Nitrofurazone group on 20th day.
  
  
  CONCLUSIONS
  To wrap up. These observations suggest that the Kombucha fungus healing quality was rapid from 12th day to the end of the research, whereas no significant difference was observed.
  The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/1107407136102196.
authors.affiliation: Department of Pathology, Faculty of Veterinary Medicine, Tehran University, Tehran, Iran. javadjavanbakht@ut.ac.ir.
authors.forename: Fardin
authors.initials: F
authors.lastname: Barati
authors.forename: Javad
authors.initials: J
authors.lastname: Javanbakht
authors.forename: Farajollah
authors.initials: F
authors.lastname: Adib-Hashemi
authors.forename: Ehsan
authors.initials: E
authors.lastname: Hosseini
authors.forename: Reyhaneh
authors.initials: R
authors.lastname: Safaeie
authors.forename: Mojtaba
authors.initials: M
authors.lastname: Rajabian
authors.forename: Mostafa
authors.initials: M
authors.lastname: Razmjoo
authors.forename: Reza
authors.initials: R
authors.lastname: Sedaghat
authors.forename: Mehdi
authors.initials: M
authors.lastname: Aghamohammad Hassan
mesh_terms: D000279:Administration, Cutaneous; D000818:Animals; D001706:Biopsy; D028241:Camellia sinensis / Q000382:microbiology*; D004195:Disease Models, Animal; D005260:Female; D005285:Fermentation; D008297:Male; D009583:Nitrofurazone / Q000008:administration & dosage / Q000494:pharmacology*; D009824:Ointments; D008517:Phytotherapy; D010946:Plants, Medicinal; D051381:Rats; D017208:Rats, Wistar; D012867:Skin / Q000187:drug effects* / Q000293:injuries / Q000382:microbiology* / Q000473:pathology; D013662:Tea / Q000382:microbiology*; D013997:Time Factors; D014945:Wound Healing / Q000187:drug effects*; D014950:Wounds, Penetrating / Q000188:drug therapy* / Q000382:microbiology* / Q000473:pathology
chemical_list: D009824:Ointments; D013662:Tea; D009583:Nitrofurazone