title: pH-induced quaternary assembly of Vitreoscilla hemoglobin: the monomer exhibits better peroxidase activity.
abstract: pH-dependent (pH6.0-8.0) quaternary structural changes of ferric Vitreoscilla hemoglobin (VHb) have been investigated using dynamic light scattering. The VHb exhibits a monomeric state under neutral conditions at pH7.0, while the protein forms distinct homodimeric species at pH6.0 and 8.0, respectively. The dissociation constant obtained using the Bio-Layer Interferometry technology indicates that, at pH7.0, the monomer-monomer dissociation of VHb is about 6-fold or 5-fold higher (KD=6.34μM) compared with that at slightly acidic pH (KD=1.05μM) or slightly alkaline pH (KD=1.22μM). The pH-dependent absorption spectra demonstrate that the heme microenvironment of VHb is sensitive to the changes of pH value. The maximum absorption band of heme group of VHb shifts from 402nm to 407nm when pH changes from 6.0 to 8.0. In addition, the fluorescence emission spectra of VHb, taken at excitation wavelength of 295nm, suggest that the single Trp122 fluorescence quantum yields in VHb are decreased due to the formation of the homodimeric species. However, the circular dichroism spectra data display that the secondary structures of VHb are little affected by pH transitions. The pH-dependent peroxidase activity of VHb was also investigated in this study. The optimum pH for VHb using 2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) as substrate is 7.0, which implies that the monomer state of VHb would exhibit better peroxidase activity than the homodimeric species of VHb at pH6.0 and 8.0.
authors.affiliation: Key Laboratory for Molecular Enzymology & Engineering, The Ministry of Education, Jilin University, Changchun 130012, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.forename: Yubin
authors.initials: Y
authors.lastname: Zhang
authors.forename: Haoran
authors.initials: H
authors.lastname: Xu
authors.forename: Lei
authors.initials: L
authors.lastname: Wu
authors.forename: Yufeng
authors.initials: Y
authors.lastname: Cao
authors.forename: Haifeng
authors.initials: H
authors.lastname: Zhao
authors.forename: Zhengqiang
authors.initials: Z
authors.lastname: Li
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics; D052160:Benzothiazoles / Q000737:chemistry; D004926:Escherichia coli / Q000235:genetics; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008027:Light; D008958:Models, Molecular; D010084:Oxidation-Reduction; D010544:Peroxidases / Q000737:chemistry* / Q000235:genetics; D055503:Protein Multimerization; D020836:Protein Structure, Quaternary; D017433:Protein Structure, Secondary; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics; D012542:Scattering, Radiation; D013451:Sulfonic Acids / Q000737:chemistry; D054793:Truncated Hemoglobins / Q000737:chemistry* / Q000235:genetics; D020589:Vitreoscilla / Q000737:chemistry*
chemical_list: D001426:Bacterial Proteins; D052160:Benzothiazoles; D011994:Recombinant Proteins; D013451:Sulfonic Acids; D054793:Truncated Hemoglobins; C082613:hemoglobin protein, Vitreoscilla; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; D010544:Peroxidases
keywords: Dimer–monomer–dimer transition; Peroxidase activity; Vitreoscilla hemoglobin