title: Swelling pressure and hydration behavior of porcine corneal stroma.
abstract: OBJECTIVE
  The aim of this study was to characterize swelling pressure-thickness, swelling pressure-hydration and hydration-thickness relations of porcine cornea.
  
  
  METHODS
  Mechanical compression tests and free swelling experiments were performed on porcine cornea. A rheometer (DHR-2, TA Instruments) with a thermally controlled fluid chamber filled with 0.9% NaCl solution was used to measure the equilibrium swelling pressure of (n = 17) corneal stromal specimens. The samples were compressed incrementally and their swelling pressure-thickness relations were obtained. In parallel to this investigation, a transient digital imaging microscope (H800-CL, American Scope Inc.), a USB autofocus camera (UM05, ViTiny), and a precision weighing scale (AGZN100, Torbal) were simultaneously used to measure the weight-thickness relation of (n = 8) corneal specimens. This experimental study gave the thickness-hydration relationship required for expressing swelling pressure measurements as a function of hydration.
  
  
  RESULTS
  At the in vivo 666 ± 68 µm central corneal thickness, an average swelling pressure of 52 ± 13 mmHg and hydration of 3.36 ± 0.25 mg H2O/mg dry tissue were found. The swelling pressure was reported as functions of both tissue thickness and hydration. The average fixed charge density of ρF/F ~ 42.8 mM and dry density of 1.47±0.15 g/cm3 were found. The thickness-hydration relationship was only linear when the tissue thickness was within the range of physiological thickness.
  
  
  CONCLUSIONS
  Overall, the physiological hydration and swelling pressure of the porcine cornea were within the same range of those reported previously for other mammalian corneas such as steers, rabbits and humans. Nevertheless, the thickness-hydration behavior of the porcine cornea was only similar to that of the human cornea.
authors.affiliation: Computational Biomechanics Laboratory, School of Mechanical and Aerospace Engineering, Oklahoma State University , Stillwater, OK , USA.
authors.forename: Hamed
authors.initials: H
authors.lastname: Hatami-Marbini
authors.forename: Ebitimi
authors.initials: E
authors.lastname: Etebu
authors.forename: Abdolrasol
authors.initials: A
authors.lastname: Rahimi
mesh_terms: D000818:Animals; D001696:Biomechanical Phenomena; D001826:Body Fluids / Q000502:physiology*; D003319:Corneal Stroma / Q000166:cytology* / Q000503:physiopathology*; D004487:Edema / Q000473:pathology* / Q000503:physiopathology*; D008954:Models, Biological; D011312:Pressure; D012212:Rheology; D013314:Stress, Mechanical; D013552:Swine; D014882:Water-Electrolyte Balance / Q000502:physiology