title: Method for measuring single-molecule adhesion forces and attachment lifetimes of protein-membrane interactions.
abstract: Many proteins that reside in the cytoplasm bind directly to cell membranes and play roles in signaling, adhesion, metabolism, cell structure, and cell motility. Several of these membrane-binding proteins, especially cytoskeletal proteins, have mechanical functions that result in the transmission of forces to the plasma membrane and organelle-membranes. Despite the importance of these interactions, remarkably little is known about the mechanical properties of the bonds between membranes and proteins. In this chapter, we describe a single-molecule, optical-trapping method for the measurement of protein-membrane adhesion forces and force-dependent attachment-lifetimes.
authors.affiliation: Department of Physiology, Pennsylvania Muscle Institute, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.
authors.forename: Serapion
authors.initials: S
authors.lastname: Pyrpassopoulos
authors.forename: Henry
authors.initials: H
authors.lastname: Shuman
authors.forename: E Michael
authors.initials: EM
authors.lastname: Ostap
mesh_terms: D002352:Carrier Proteins; D002448:Cell Adhesion / Q000235:genetics; D002462:Cell Membrane / Q000737:chemistry / Q000378:metabolism*; D003593:Cytoplasm / Q000737:chemistry* / Q000378:metabolism; D003598:Cytoskeletal Proteins / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D008967:Molecular Biology / Q000379:methods*; D052898:Optical Tweezers; D011506:Proteins / Q000737:chemistry / Q000378:metabolism*
chemical_list: D002352:Carrier Proteins; D003598:Cytoskeletal Proteins; D011506:Proteins