title: Microfluidic integrated optoelectronic tweezers for single-cell preparation and analysis.
abstract: We report a novel microfluidic integrated optoelectronic tweezers (OET) platform for single-cell sample preparation and analysis. Integration of OET and microfluidics is achieved by embedding single-wall carbon nanotube (SWNT) electrodes into multilayer PDMS structures. This integrated platform allows users to selectively pick up individual cells from a population with light beams based on their optical signatures such as size, shape, and fluorescence, and transport them into isolated chambers using light induced dielectrophoretic forces. Isolated cells can be encapsulated into nanoliter liquid plugs and transported out of the platform for downstream molecule analysis using standard commercial instruments.
authors.affiliation: Department of Mechanical and Aerospace Engineering, University of California, Los Angeles, CA 90095, USA.
authors.forename: Kuo-Wei
authors.initials: KW
authors.lastname: Huang
authors.forename: Yi-Chien
authors.initials: YC
authors.lastname: Wu
authors.forename: Ji-Ann
authors.initials: JA
authors.lastname: Lee
authors.forename: Pei-Yu
authors.initials: PY
authors.lastname: Chiou
mesh_terms: D004129:Dimethylpolysiloxanes / Q000737:chemistry; D004566:Electrodes; D006367:HeLa Cells; D006801:Humans; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods; D037742:Nanotubes, Carbon / Q000737:chemistry; D052898:Optical Tweezers; D012333:RNA, Messenger / Q000032:analysis; D060888:Real-Time Polymerase Chain Reaction; D059010:Single-Cell Analysis
chemical_list: D004129:Dimethylpolysiloxanes; D037742:Nanotubes, Carbon; D012333:RNA, Messenger; C013830:baysilon