title: Protective effect of extract of Crataegus pinnatifida pollen on DNA damage response to oxidative stress.
abstract: The protective effect of extract of Crataegus pinnatifida (Rosaceae) pollen (ECPP) on the DNA damage response to oxidative stress was investigated and assessed with an alkaline single-cell gel electrophoresis (SCGE) assay and pBR322 plasmid DNA breaks in site-specific and non-site-specific systems. Total phenolic content, total flavonoid content, individual phenolic compounds, antioxidant activities (1,1-diphenyl-2-picrylhydrazyl (DPPH), radical scavenging activity, FRAP, and chelating activity) were also determined. The results showed that ECPP possessed a strong ability to protect DNA from being damaged by hydroxyl radicals in both the site-specific system and the non-site-specific system. It also exhibited a cytoprotection effect in mouse lymphocytes against H₂O₂-induced DNA damage. These protective effects may be related to its high total phenolic content (17.65±0.97 mg GAE/g), total flavonoid content (8.04±0.97 mg rutin/g), strong free radical scavenging activity and considerable ferrous ion chelating ability (14.48±0.21 mg Na₂EDTA/g).
authors.affiliation: Institute of Analytical Science, Shaanxi Provincial Key Laboratory of Electroanalytical Chemistry, Northwest University, Xi'an City 710069, China.
authors.forename: Ni
authors.initials: N
authors.lastname: Cheng
authors.forename: Yuan
authors.initials: Y
authors.lastname: Wang
authors.forename: Hui
authors.initials: H
authors.lastname: Gao
authors.forename: Jialing
authors.initials: J
authors.lastname: Yuan
authors.forename: Fan
authors.initials: F
authors.lastname: Feng
authors.forename: Wei
authors.initials: W
authors.lastname: Cao
authors.forename: Jianbin
authors.initials: J
authors.lastname: Zheng
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D001688:Biological Products / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D020552:Comet Assay; D027825:Crataegus / Q000737:chemistry*; D053960:DNA Breaks / Q000187:drug effects; D004249:DNA Damage / Q000187:drug effects*; D020834:Ethnopharmacology; D005419:Flavonoids / Q000032:analysis / Q000302:isolation & purification / Q000494:pharmacology; D016166:Free Radical Scavengers / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D007502:Iron Chelating Agents / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D008214:Lymphocytes / Q000187:drug effects* / Q000378:metabolism; D008516:Medicine, Chinese Traditional; D051379:Mice; D008815:Mice, Inbred Strains; D018384:Oxidative Stress / Q000187:drug effects*; D010636:Phenols / Q000032:analysis / Q000302:isolation & purification / Q000494:pharmacology; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D011058:Pollen / Q000737:chemistry*; D011429:Propolis / Q000737:chemistry
chemical_list: D000975:Antioxidants; D001688:Biological Products; D005419:Flavonoids; D016166:Free Radical Scavengers; D007502:Iron Chelating Agents; D010636:Phenols; D010936:Plant Extracts; D011429:Propolis
keywords: DNA damage; Extract of Crataegus pinnatifida pollen (ECPP); Oxidative stress; Phenolic compounds