title: Silicon dioxide thin film mediated single cell nucleic acid isolation.
abstract: A limited amount of DNA extracted from single cells, and the development of single cell diagnostics make it necessary to create a new highly effective method for the single cells nucleic acids isolation. In this paper, we propose the DNA isolation method from biomaterials with limited DNA quantity in sample, and from samples with degradable DNA based on the use of solid-phase adsorbent silicon dioxide nanofilm deposited on the inner surface of PCR tube.
authors.affiliation: Immanuel Kant Baltic Federal University, Kaliningrad, Russia. eubogdanov@gmail.com
authors.forename: Evgeny
authors.initials: E
authors.lastname: Bogdanov
authors.forename: Irina
authors.initials: I
authors.lastname: Dominova
authors.forename: Natalia
authors.initials: N
authors.lastname: Shusharina
authors.forename: Stepan
authors.initials: S
authors.lastname: Botman
authors.forename: Vitaliy
authors.initials: V
authors.lastname: Kasymov
authors.forename: Maksim
authors.initials: M
authors.lastname: Patrushev
mesh_terms: D000818:Animals; D004247:DNA / Q000302:isolation & purification; D004209:Disposable Equipment; D005260:Female; D006801:Humans; D008567:Membranes, Artificial; D009696:Nucleic Acids / Q000302:isolation & purification*; D009865:Oocytes / Q000378:metabolism; D010969:Plastics / Q000737:chemistry; D016133:Polymerase Chain Reaction / Q000295:instrumentation / Q000379:methods; D011126:Polypropylenes / Q000737:chemistry; D051381:Rats; D012822:Silicon Dioxide / Q000737:chemistry*; D059010:Single-Cell Analysis / Q000295:instrumentation / Q000379:methods
chemical_list: D008567:Membranes, Artificial; D009696:Nucleic Acids; D010969:Plastics; D011126:Polypropylenes; D012822:Silicon Dioxide; D004247:DNA