title: Description of a double centrifugation tube method for concentrating canine platelets.
abstract: BACKGROUND
  To evaluate the efficiency of platelet-rich plasma preparations by means of a double centrifugation tube method to obtain platelet-rich canine plasma at a concentration at least 4 times higher than the baseline value and a concentration of white blood cells not exceeding twice the reference range. A complete blood count was carried out for each sample and each concentrate. Whole blood samples were collected from 12 clinically healthy dogs (consenting blood donors). Blood was processed by a double centrifugation tube method to obtain platelet concentrates, which were then analyzed by a flow cytometry haematology system for haemogram. Platelet concentration and white blood cell count were determined in all samples.
  
  
  RESULTS
  Platelet concentration at least 4 times higher than the baseline value and a white blood cell count not exceeding twice the reference range were obtained respectively in 10 cases out of 12 (83.3%) and 11 cases out of 12 (91.6%).
  
  
  CONCLUSIONS
  This double centrifugation tube method is a relatively simple and inexpensive method for obtaining platelet-rich canine plasma, potentially available for therapeutic use to improve the healing process.
authors.affiliation: Department of Animal Medicine, Production and Health, University of Padova, Padova, Italy. anna.perazzi@unipd.it
authors.forename: Anna
authors.initials: A
authors.lastname: Perazzi
authors.forename: Roberto
authors.initials: R
authors.lastname: Busetto
authors.forename: Tiziana
authors.initials: T
authors.lastname: Martinello
authors.forename: Michele
authors.initials: M
authors.lastname: Drigo
authors.forename: Daniela
authors.initials: D
authors.lastname: Pasotto
authors.forename: Francesco
authors.initials: F
authors.lastname: Cian
authors.forename: Marco
authors.initials: M
authors.lastname: Patruno
authors.forename: Ilaria
authors.initials: I
authors.lastname: Iacopetti
mesh_terms: D000818:Animals; D001792:Blood Platelets / Q000378:metabolism*; D002469:Cell Separation / Q000379:methods / Q000662:veterinary*; D002498:Centrifugation / Q000379:methods / Q000662:veterinary*; D004285:Dogs / Q000097:blood*; D005260:Female; D005434:Flow Cytometry / Q000662:veterinary; D007958:Leukocyte Count / Q000662:veterinary; D008297:Male; D010976:Platelet Count / Q000662:veterinary; D053657:Platelet-Rich Plasma / Q000378:metabolism