title: Tooth tissue engineering: the influence of hydrophilic surface on nanocrystalline diamond films for human dental stem cells.
abstract: New techniques for tissue engineering (TE) are rapidly emerging. The basic concept of autologous TE is to isolate cells from small biopsy specimens, and to expand these cells in culture for subsequent seeding onto biodegradable scaffolds. Nanocrystalline diamond films have attracted the attention of researchers from a variety of different areas in recent years, due to their unique and exceptional properties. In this approach, human dental stem cells (hDSCs) were characterized by flow cytometry and grown on diamond films with hydrogen (H)-terminated and oxygen (O)-terminated surfaces for 28 days, and then removed by lysis and washing with distilled water. Energy dispersive spectroscopy analysis was performed, showing that the regions with O-terminated surfaces contained much higher levels of deposited calcium, oxygen, and phosphorus. These results suggest that the extracellular matrix was considerably more developed in the O-terminated regions, as compared with the H-terminated regions. In addition, optical microscopy of hDSCs cultured on the diamond substrate with H- and O-terminated surfaces, before washing with distilled water, showed preferential directions of the cells arrangement, where orthogonal lines suggest that the cells appeared to be following the O-terminated regions or hydrophilic surface. These findings suggest that O-terminated diamond surfaces prepared on biodegradable scaffolds can be useful for mineralized dental tissue formation.
authors.affiliation: 1 Department of Medicine, Division of Plastic Surgery, UNIFESP-CTCMol-Federal University of São Paulo, Center of Cellular and Molecular Therapy, São Paulo, Brazil .
authors.forename: Silvio Eduardo
authors.initials: SE
authors.lastname: Duailibi
authors.forename: Monica Talarico
authors.initials: MT
authors.lastname: Duailibi
authors.forename: Lydia Masako
authors.initials: LM
authors.lastname: Ferreira
authors.forename: Karina Inacio Ladislau Carvalho
authors.initials: KI
authors.lastname: Salmazi
authors.forename: Maria Cecília
authors.initials: MC
authors.lastname: Salvadori
authors.forename: Fernanda
authors.initials: F
authors.lastname: de Sá Teixeira
authors.forename: Alberto
authors.initials: A
authors.lastname: Pasquarelli
authors.forename: Joseph Phillip
authors.initials: JP
authors.lastname: Vacanti
authors.forename: Pamela Crotty
authors.initials: PC
authors.lastname: Yelick
mesh_terms: D002478:Cells, Cultured; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D058612:Nanodiamonds / Q000737:chemistry*; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry*; D014070:Tooth / Q000166:cytology* / Q000378:metabolism
chemical_list: D058612:Nanodiamonds