title: The role of ion mobility spectrometry-mass spectrometry in the analysis of protein reference standards.
abstract: To achieve comparability of measurement results of protein amount of substance content between clinical laboratories, suitable reference materials are required. The impact on measurement comparability of potential differences in the tertiary and quaternary structure of protein reference standards is as yet not well understood. With the use of human growth hormone as a model protein, the potential of ion mobility spectrometry-mass spectrometry as a tool to assess differences in the structure of protein reference materials and their interactions with antibodies has been investigated here.
authors.affiliation: Astbury Centre for Structural Molecular Biology, University of Leeds, Leeds, United Kingdom. caroline.pritchard@lgcgroup.com
authors.forename: Caroline
authors.initials: C
authors.lastname: Pritchard
authors.forename: Gavin
authors.initials: G
authors.lastname: O'Connor
authors.forename: Alison E
authors.initials: AE
authors.lastname: Ashcroft
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000918:Antibody Specificity; D002417:Cattle; D019382:Human Growth Hormone / Q000032:analysis* / Q000737:chemistry* / Q000276:immunology; D006801:Humans; D013058:Mass Spectrometry / Q000592:standards*; D011489:Protein Denaturation; D012015:Reference Standards; D012710:Serum Albumin, Bovine / Q000032:analysis
chemical_list: D000911:Antibodies, Monoclonal; D019382:Human Growth Hormone; D012710:Serum Albumin, Bovine