title: Quantitative proteomics reveals diverse roles of miR-148a from gastric cancer progression to neurological development.
abstract: MicroRNAs (miRNAs) are noncoding RNAs that control gene expression either by degradation of mRNAs or inhibition of protein translation. miR-148a has been reported to have the impacts on tumor progression. Here, a quantitative proteomics combined with stable isotope labeling was applied to identify the global profile of miR-148a-regulated downstream proteins. The data have been deposited to the ProteomeXchange with identifier PXD000190. A total of 2938 proteins were quantified, and 55 proteins were considered to be regulated by miR-148a. We found that not only proteins associated with cancer progression but also molecules involved in neural development were regulated by miR-148a. This study is the first to identify the function of miR-148a in neural development by using a proteomic approach. Analysis of a public clinical database also showed that the patients with neural diseases could display abnormal expression of miR-148a. Moreover, silencing of miR-148a led to the abnormal morphology and decreased expression of neuron-related markers in the developing brain of zebrafish. These results provided important insight into the regulation of neurological development elicited by miR-148a.
authors.affiliation: Institute of Molecular and Cellular Biology and Department of Life Science, National Taiwan University, Taipei 106, Taiwan.
authors.forename: Chia-Wei
authors.initials: CW
authors.lastname: Hu
authors.forename: Chien-Wei
authors.initials: CW
authors.lastname: Tseng
authors.forename: Chih-Wei
authors.initials: CW
authors.lastname: Chien
authors.forename: Hsuan-Cheng
authors.initials: HC
authors.lastname: Huang
authors.forename: Wei-Chi
authors.initials: WC
authors.lastname: Ku
authors.forename: Shyh-Jye
authors.initials: SJ
authors.lastname: Lee
authors.forename: Yu-Ju
authors.initials: YJ
authors.lastname: Chen
authors.forename: Hsueh-Fen
authors.initials: HF
authors.lastname: Juan
mesh_terms: D000544:Alzheimer Disease / Q000235:genetics / Q000378:metabolism; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000196:embryology / Q000378:metabolism; D016022:Case-Control Studies; D045744:Cell Line, Tumor; D018450:Disease Progression; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D035683:MicroRNAs / Q000502:physiology*; D010300:Parkinson Disease / Q000235:genetics / Q000378:metabolism; D040901:Proteomics; D034622:RNA Interference; D012689:Sequence Homology, Nucleic Acid; D013116:Spinal Cord / Q000196:embryology / Q000378:metabolism; D013274:Stomach Neoplasms; D059467:Transcriptome; D015027:Zebrafish
chemical_list: C550570:MIRN148 microRNA, human; D035683:MicroRNAs