title: Simultaneous determination of eighteen steroid hormones in antler velvet by gas chromatography-tandem mass spectrometry.
abstract: A method for the simultaneous determination of eighteen steroid hormones, including 17α-ethinylestradiol, 17α-estradiol, 19-nortestosterone, estriol, testosterone, androsterone, 17β-estradiol, estrone, progesterone, 17α-hydroxyprogesterone, medroxyprogesterone, norethisterone acetate, testosterone 17-propionate, medroxyprogesterone-17-acetate, corticosterone, megestrol-17-acetate, chlromadinone-17-acetate and 17β-estradiol-benzoate, in antler velvet was developed by solid-phase extraction (SPE) and gas chromatography-tandem mass spectrometry (GC-MS/MS). Prior to the determination by GC-MS/MS, the analytes were derivatized with heptafluorobutyric acid anhydrides (HFBA). The recoveries were in the range of 62.13-104.00% for eighteen compounds at concentration levels (5-500 μg/L) and relative standard deviations (RSDs) were lower than 17.15%. The detection limits were from 0.2 to 22.3 μg/kg for the steroid hormones. The developed method was rapid, sensitive and successfully applied to the determination of steroid hormones in antler velvet samples collected from three countries.
authors.affiliation: College of Chemistry, Jilin University, Changchun 130012, PR China.
authors.forename: Chunmei
authors.initials: C
authors.lastname: Lu
authors.forename: Mingtai
authors.initials: M
authors.lastname: Wang
authors.forename: Jun
authors.initials: J
authors.lastname: Mu
authors.forename: Dachuan
authors.initials: D
authors.lastname: Han
authors.forename: Yuping
authors.initials: Y
authors.lastname: Bai
authors.forename: Hanqi
authors.initials: H
authors.lastname: Zhang
mesh_terms: D000818:Animals; D000999:Antlers / Q000737:chemistry*; D003670:Deer; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D015394:Molecular Structure; D013256:Steroids / Q000737:chemistry*
chemical_list: D013256:Steroids
keywords: Antler velvet; Determination; Gas chromatography–mass spectrometry/mass spectrometry; Steroid hormones