title: Developmental stage dependent neural stem cells sensitivity to methylmercury chloride on different biofunctional surfaces.
abstract: Sensitivity of neural stem cells viability, proliferation and differentiation upon exposure to methylmercury chloride (MeHgCl) was investigated on different types of biofunctional surfaces. Patterns of biodomains created by microprinting/microspotting of poly-l-lysine or extracellular matrix proteins (fibronectin and vitronectin) allowed for non-specific electrostatic or specific, receptor mediated interactions, respectively, between stem cells and the surface. The neural stem cell line HUCB-NSC has been previously shown to be susceptible to MeHgCl in developmentally dependent manner. Here we demonstrated that developmental sensitivity of HUCB-NSC to MeHgCl depends upon the type of adhesive biomolecules and the geometry of biodomains. Proliferation of HUCB-NSC was diminished in time and MeHgCl concentration dependent manner. In addition, the response to MeHgCl was found to be cell-type dependent. Undifferentiated cells were the most sensitive independently of the type of bioactive domain. Significant decrease of GFAP+ cells was detected among cells growing on poly-l-lysine, while on fibronectin and vitronectin, this effect was observed only in the highest (1μM) concentration of MeHgCl. β-Tubulin III expressing cells were most sensitive on fibronectin domains. In addition, limited bioactive domains to μm in size, as compared to non-patterned larger area of the same adhesive substrate, exerted protective role. Thus, the surface area and type of cell/biofunctional surface interaction exerted significant influence on developmental stage and cell-type specific response of HUCB-NSC to MeHgCl.
authors.affiliation: Mossakowski Medical Research Centre, Polish Academy of Sciences, NeuroRepair Department, Warsaw, Poland.
authors.forename: M
authors.initials: M
authors.lastname: Zychowicz
authors.forename: D
authors.initials: D
authors.lastname: Dziedzicka
authors.forename: D
authors.initials: D
authors.lastname: Mehn
authors.forename: H
authors.initials: H
authors.lastname: Kozlowska
authors.forename: A
authors.initials: A
authors.lastname: Kinsner-Ovaskainen
authors.forename: P P
authors.initials: PP
authors.lastname: Stępień
authors.forename: F
authors.initials: F
authors.lastname: Rossi
authors.forename: L
authors.initials: L
authors.lastname: Buzanska
mesh_terms: D002454:Cell Differentiation / Q000187:drug effects*; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005353:Fibronectins / Q000737:chemistry; D006801:Humans; D008767:Methylmercury Compounds / Q000008:administration & dosage / Q000633:toxicity*; D058953:Neural Stem Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D020258:Neurotoxicity Syndromes / Q000209:etiology; D011107:Polylysine / Q000737:chemistry; D013997:Time Factors; D019096:Vitronectin / Q000737:chemistry
chemical_list: D005353:Fibronectins; D008767:Methylmercury Compounds; D019096:Vitronectin; D011107:Polylysine; C004925:methylmercuric chloride
keywords: Bioactive domains; Developmental neurotoxicity; MeHgCl sensitivity; Neural stem cells