title: Effects of electromagnetic fields on osteogenesis of human alveolar bone-derived mesenchymal stem cells.
abstract: This study was performed to investigate the effects of extremely low frequency pulsed electromagnetic fields (ELF-PEMFs) on the proliferation and differentiation of human alveolar bone-derived mesenchymal stem cells (hABMSCs). Osteogenesis is a complex series of events involving the differentiation of mesenchymal stem cells to generate new bone. In this study, we examined not merely the effect of ELF-PEMFs on cell proliferation, alkaline phosphatase (ALP) activity, and mineralization of the extracellular matrix but vinculin, vimentin, and calmodulin (CaM) expressions in hABMSCs during osteogenic differentiation. Exposure of hABMSCs to ELF-PEMFs increased proliferation by 15% compared to untreated cells at day 5. In addition, exposure to ELF-PEMFs significantly increased ALP expression during the early stages of osteogenesis and substantially enhanced mineralization near the midpoint of osteogenesis within 2 weeks. ELF-PEMFs also increased vinculin, vimentin, and CaM expressions, compared to control. In particular, CaM indicated that ELF-PEMFs significantly altered the expression of osteogenesis-related genes. The results indicated that ELF-PEMFs could enhance early cell proliferation in hABMSCs-mediated osteogenesis and accelerate the osteogenesis.
authors.affiliation: Department of Biosystems & Biomaterials Science and Engineering, Seoul National University, Seoul 151-921, Republic of Korea.
authors.forename: KiTaek
authors.initials: K
authors.lastname: Lim
authors.forename: Jin
authors.initials: J
authors.lastname: Hexiu
authors.forename: Jangho
authors.initials: J
authors.lastname: Kim
authors.forename: Hoon
authors.initials: H
authors.lastname: Seonwoo
authors.forename: Woo Jae
authors.initials: WJ
authors.lastname: Cho
authors.forename: Pill-Hoon
authors.initials: PH
authors.lastname: Choung
authors.forename: Jong Hoon
authors.initials: JH
authors.lastname: Chung
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000539:Alveolar Process / Q000166:cytology*; D002147:Calmodulin / Q000378:metabolism; D002448:Cell Adhesion; D002454:Cell Differentiation; D049109:Cell Proliferation; D048430:Cell Shape; D002470:Cell Survival; D002478:Cells, Cultured; D004574:Electromagnetic Fields; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D016348:Osseointegration; D015675:Osteocalcin / Q000378:metabolism; D010012:Osteogenesis; D014746:Vimentin / Q000378:metabolism; D016596:Vinculin / Q000378:metabolism
chemical_list: D002147:Calmodulin; D014746:Vimentin; D015675:Osteocalcin; D016596:Vinculin; D000469:Alkaline Phosphatase