title: Clostridium acidurici electron-bifurcating formate dehydrogenase.
abstract: Cell extracts of uric acid-grown Clostridium acidurici catalyzed the coupled reduction of NAD(+) and ferredoxin with formate at a specific activity of 1.3 U/mg. The enzyme complex catalyzing the electron-bifurcating reaction was purified 130-fold and found to be composed of four subunits encoded by the gene cluster hylCBA-fdhF2.
authors.affiliation: Max Planck Institute for Terrestrial Microbiology, Marburg, Germany.
authors.forename: Shuning
authors.initials: S
authors.lastname: Wang
authors.forename: Haiyan
authors.initials: H
authors.lastname: Huang
authors.forename: Jörg
authors.initials: J
authors.lastname: Kahnt
authors.forename: Rudolf K
authors.initials: RK
authors.lastname: Thauer
mesh_terms: D003013:Clostridium / Q000201:enzymology*; D005288:Ferredoxins / Q000378:metabolism*; D005560:Formate Dehydrogenases / Q000302:isolation & purification* / Q000378:metabolism*; D005561:Formates / Q000378:metabolism*; D005810:Multigene Family; D009243:NAD / Q000378:metabolism*; D055503:Protein Multimerization; D021122:Protein Subunits / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D005288:Ferredoxins; D005561:Formates; D021122:Protein Subunits; D009243:NAD; C030544:formic acid; D005560:Formate Dehydrogenases