title: Biogenic silver nanoparticles with chlorogenic acid as a bioreducing agent.
abstract: We report the synthesis of biogenic silver nanoparticles using chlorogenic acid as a bioreducing agent. Chlorogenic acid is a polyphenol compound abundant in coffee. UV-Vis spectra showed the characteristic surface plasmon resonance band at 415 nm, indicating the successful synthesis of biogenic silver nanoparticles. Spherical and irregular shaped nanoparticles were observed with an average diameter of 19.29 +/- 8.23 nm. The reaction yield from silver ion to silver nanoparticles was observed as 95.43% by using inductively coupled plasma-mass spectrometry. Fourier transform infrared spectra revealed that the -C = O groups of chlorogenic acid may coordinate or complex into silver nanoparticles. Biogenic silver nanoparticles exerted higher antibacterial activity against Gram-negative bacteria than against Gram-positive bacteria. Interestingly, a comparable antibacterial activity to a standard antibiotic was observed against two strains of Pseudomonas aeruginosa (minimum inhibitory concentration of 0.66 microg/mL). The synergistic effect of a combination of silver nanoparticles and chlorogenic acid on antibacterial activity is obvious, leading to approximately 8-fold enhancement in the case of Pseudomonas aeruginosa when compared with chlorogenic acid alone. The present report suggests that a pure compound with a plant origin is capable of being a bioreducing agent for the synthesis of biogenic silver nanoparticles with superior antibacterial activity, opening up many applications in nanomedicine and nanobiotechnology.
authors.affiliation: College of Pharmacy, Inje University, 607 Obang-dong, Gimhae, Gyeongnam 621-749, Republic of Korea
authors.forename: Hwa Jung
authors.initials: HJ
authors.lastname: Noh
authors.forename: Hyun-Seok
authors.initials: HS
authors.lastname: Kim
authors.forename: Sang Hui
authors.initials: SH
authors.lastname: Jun
authors.forename: Young-Hwa
authors.initials: YH
authors.lastname: Kang
authors.forename: Seonho
authors.initials: S
authors.lastname: Cho
authors.forename: Youmie
authors.initials: Y
authors.lastname: Park
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D002244:Carbon / Q000737:chemistry; D002726:Chlorogenic Acid / Q000737:chemistry*; D013058:Mass Spectrometry; D053768:Metal Nanoparticles / Q000737:chemistry*; D008826:Microbial Sensitivity Tests; D018625:Microscopy, Atomic Force; D046529:Microscopy, Electron, Transmission; D050997:Nanomedicine / Q000379:methods; D036103:Nanotechnology / Q000379:methods; D010100:Oxygen / Q000737:chemistry; D010316:Particle Size; D011550:Pseudomonas aeruginosa / Q000187:drug effects; D019163:Reducing Agents / Q000494:pharmacology; D012834:Silver / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D000900:Anti-Bacterial Agents; D019163:Reducing Agents; D002726:Chlorogenic Acid; D012834:Silver; D002244:Carbon; D010100:Oxygen