title: Polymorphisms, de novo lipogenesis, and plasma triglyceride response following fish oil supplementation.
abstract: Interindividual variability in the response of plasma triglyceride concentrations (TG) following fish oil consumption has been observed. Our objective was to examine the associations between single-nucleotide polymorphisms (SNPs) within genes encoding proteins involved in de novo lipogenesis and the relative change in plasma TG levels following a fish oil supplementation. Two hundred and eight participants were recruited in the greater Quebec City area. The participants completed a six-week fish oil supplementation (5 g fish oil/day: 1.9-2.2 g eicosapentaenoic acid and 1.1 g docosahexaenoic acid. SNPs within SREBF1, ACLY, and ACACA genes were genotyped using TAQMAN methodology. After correction for multiple comparison, only two SNPs, rs8071753 (ACLY) and rs1714987 (ACACA), were associated with the relative change in plasma TG concentrations (P = 0.004 and P = 0.005, respectively). These two SNPs explained 7.73% of the variance in plasma TG relative change following fish oil consumption. Genotype frequencies of rs8071753 according to the TG response groups (responders versus nonresponders) were different (P = 0.02). We conclude that the presence of certain SNPs within genes, such as ACLY and ACACA, encoding proteins involved in de novo lipogenesis seem to influence the plasma TG response following fish oil consumption.
authors.affiliation: Institute of Nutrition and Functional Foods (INAF) Laval University, Quebec, G1V 0A6, Canada.
authors.forename: Annie
authors.initials: A
authors.lastname: Bouchard-Mercier
authors.forename: Iwona
authors.initials: I
authors.lastname: Rudkowska
authors.forename: Simone
authors.initials: S
authors.lastname: Lemieux
authors.forename: Patrick
authors.initials: P
authors.lastname: Couture
authors.forename: Marie-Claude
authors.initials: MC
authors.lastname: Vohl
mesh_terms: D001275:ATP Citrate (pro-S)-Lyase / Q000235:genetics*; D000103:Acetyl-CoA Carboxylase / Q000235:genetics*; D000328:Adult; D002318:Cardiovascular Diseases / Q000517:prevention & control; D019587:Dietary Supplements; D015525:Fatty Acids, Omega-3 / Q000008:administration & dosage*; D005260:Female; D005395:Fish Oils / Q000008:administration & dosage; D005787:Gene Frequency; D056726:Genetic Association Studies; D006239:Haplotypes; D006801:Humans; D015810:Linkage Disequilibrium; D050155:Lipogenesis / Q000235:genetics*; D008297:Male; D020641:Polymorphism, Single Nucleotide; D051780:Sterol Regulatory Element Binding Protein 1 / Q000235:genetics; D016896:Treatment Outcome; D014280:Triglycerides / Q000097:blood*; D055815:Young Adult
chemical_list: D015525:Fatty Acids, Omega-3; D005395:Fish Oils; C497387:SREBF1 protein, human; D051780:Sterol Regulatory Element Binding Protein 1; D014280:Triglycerides; D001275:ATP Citrate (pro-S)-Lyase; D000103:Acetyl-CoA Carboxylase
keywords: ACACA; ACLY; SREBF1; fatty acid biosynthesis; interindividual variability; omega-3 polyunsaturated fatty acids