title: MicroRNA-26a promotes tumor growth and angiogenesis in glioma by directly targeting prohibitin.
abstract: OBJECTIVE
  Glioma accounts for the majority of primary malignant brain tumors in adults. Upregulation of microRNA-26a (miR-26a) has been observed in glioma. However, the biological function and molecular mechanism of miR-26a in glioma remain to be elucidated.
  
  
  METHODS
  Glioma cells stably overexpressing or down-expressing miR-26a were analyzed for both in vitro and in vivo biological functions. Novel target of miR-26a was identified by bioinformatics searching and molecular biological assays. Glioma specimens and normal brain tissues were analyzed for both expression levels of miR-26a and its target.
  
  
  RESULTS
  Forced expression of miR-26a in glioma cells significantly increased both growth rate and colony formation in vitro and tumor growth and angiogenesis in vivo, while reduced expression of miR-26a played opposite roles. MiR-26a directly targeted prohibitin (PHB) whose expression levels were downregulated in glioma specimens. The levels of miR-26a were inversely correlated with PHB expression levels in glioma samples and strongly correlated with clinical WHO grades of glioma.
  
  
  CONCLUSIONS
  These results reveal that miR-26a regulates PHB and promotes glioma progression both in vitro and in vivo and that miR-26a and its target PHB are associated with glioma development, which can be helpful in developing microRNA-based treatment for glioma in the future.
authors.affiliation: Department of Pathology, Jiangsu Key Lab of Cancer Biomarkers, Prevention and Treatment, Cancer Center, Nanjing Medical University, Nanjing, China.
authors.forename: Xu
authors.initials: X
authors.lastname: Qian
authors.forename: Peng
authors.initials: P
authors.lastname: Zhao
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.forename: Zhu-Mei
authors.initials: ZM
authors.lastname: Shi
authors.forename: Lin
authors.initials: L
authors.lastname: Wang
authors.forename: Qing
authors.initials: Q
authors.lastname: Xu
authors.forename: Min
authors.initials: M
authors.lastname: Wang
authors.forename: Ning
authors.initials: N
authors.lastname: Liu
authors.forename: Ling-Zhi
authors.initials: LZ
authors.lastname: Liu
authors.forename: Bing-Hua
authors.initials: BH
authors.lastname: Jiang
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D018450:Disease Progression; D005910:Glioma / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D035683:MicroRNAs / Q000096:biosynthesis*; D009389:Neovascularization, Pathologic / Q000378:metabolism*; D000091402:Prohibitins; D036223:Receptor, EphB3; D012097:Repressor Proteins / Q000096:biosynthesis*
chemical_list: D035683:MicroRNAs; D000091402:Prohibitins; D036223:Receptor, EphB3; D012097:Repressor Proteins; C528163:MIRN26 microRNA, human; C000717328:PHB protein, human
keywords: Angiogenesis; Glioma; MicroRNA-26a; Prohibitin; Tumor growth