title: Construction and analysis of a subtractive cDNA library of early embryonic development in duck.
abstract: Several studies have documented the process of early embryonic development in poultry; however, the molecular mechanisms underlying its developmental regulation are poorly understood, particularly in ducks. In this study, we analyzed differential gene expression of embryos 6 and 25 h following oviposition to determine which genes regulate the early developmental stage in ducks. Among 216 randomly selected clones, 39 protein-encoding cDNAs that function in metabolism, transcription, transportation, proliferation/apoptosis, cell cycle, cell adhesion, and methylation were identified. Additionally, the full-length cDNA of the Nanog gene, encoding a 302-amino acid protein, was obtained. Quantitative real-time polymerase chain reaction analyses were performed to detect expression levels of the selected genes during early and late embryonic stages, which revealed that these genes are expressed in a particular spatial and temporal pattern. These results indicate that these genes may play pivotal roles in the process of area pellucida formation through a complex and precise regulatory network during development in duck embryos.
authors.affiliation: College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China.
authors.forename: Y L
authors.initials: YL
authors.lastname: Liu
authors.forename: L X
authors.initials: LX
authors.lastname: Zhong
authors.forename: J J
authors.initials: JJ
authors.lastname: Li
authors.forename: J D
authors.initials: JD
authors.lastname: Shen
authors.forename: D Q
authors.initials: DQ
authors.lastname: Wang
authors.forename: Z R
authors.initials: ZR
authors.lastname: Tao
authors.forename: F X
authors.initials: FX
authors.lastname: Shi
authors.forename: L Z
authors.initials: LZ
authors.lastname: Lu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D004372:Ducks / Q000196:embryology / Q000235:genetics* / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D015723:Gene Library; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data
chemical_list: D018398:Homeodomain Proteins