title: Detection of New Delhi metallo-beta-lactamase and extended-spectrum beta-lactamase genes in Escherichia coli isolated from mastitic milk samples.
abstract: In this study, eight Escherichia coli isolates were obtained from milk samples of dairy cattle suffering from clinical/subclinical mastitis. Isolates were characterized for antimicrobial resistance traits and virulence genes. Results revealed that one isolate was harbouring New Delhi metallo-beta-lactamase gene (blaNDM ). Cloning and sequencing of the PCR amplicon confirmed the identity of the gene (GenBank accession no. KC769583) having 100% homology with blaNDM-5 (GenBank accession no. JN104597.1), and this isolate was susceptible to colistin, chloramphenicol and tetracycline only. Moreover, another isolate carried extended-spectrum beta-lactamase (ESBL) gene - blaCTX-M , and all isolates possessed blaTEM gene. Of the eight isolates, only one isolate was positive for shiga toxin gene (stx2), and none were harbouring stx1 gene. Occurrence of New Delhi metallo-beta-lactamase (blaNDM ) in one E. coli isolate and ESBL genes in other isolates poses a potential threat to human health following possible entry and spread through food chain.
authors.affiliation: Division of Animal Health, ICAR Research Complex for NEH Region, Umiam, India. ghataksnd@rediffmail.com
authors.forename: S
authors.initials: S
authors.lastname: Ghatak
authors.forename: A
authors.initials: A
authors.lastname: Singha
authors.forename: A
authors.initials: A
authors.lastname: Sen
authors.forename: C
authors.initials: C
authors.lastname: Guha
authors.forename: A
authors.initials: A
authors.lastname: Ahuja
authors.forename: U
authors.initials: U
authors.lastname: Bhattacharjee
authors.forename: S
authors.initials: S
authors.lastname: Das
authors.forename: N R
authors.initials: NR
authors.lastname: Pradhan
authors.forename: K
authors.initials: K
authors.lastname: Puro
authors.forename: C
authors.initials: C
authors.lastname: Jana
authors.forename: T K
authors.initials: TK
authors.lastname: Dey
authors.forename: K L
authors.initials: KL
authors.lastname: Prashantkumar
authors.forename: A
authors.initials: A
authors.lastname: Das
authors.forename: I
authors.initials: I
authors.lastname: Shakuntala
authors.forename: U
authors.initials: U
authors.lastname: Biswas
authors.forename: P S
authors.initials: PS
authors.lastname: Jana
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002417:Cattle; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology / Q000235:genetics* / Q000302:isolation & purification; D004927:Escherichia coli Infections / Q000382:microbiology / Q000662:veterinary*; D005260:Female; D005798:Genes, Bacterial; D006801:Humans; D007194:India; D008414:Mastitis, Bovine / Q000382:microbiology*; D008826:Microbial Sensitivity Tests; D008892:Milk / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D001618:beta-Lactamases / Q000235:genetics*
chemical_list: D000900:Anti-Bacterial Agents; D001618:beta-Lactamases; C552864:beta-lactamase NDM-1
keywords: Escherichia coli; extended-spectrum beta-lactamase; metallo-beta-lactamase; milk