title: Expression pattern of zinc-finger transcription factor Odd-skipped related 2 in murine development and neonatal stage.
abstract: The Odd-skipped gene, first identified as a Drosophila pair-rule zinc-finger transcription factor, plays an important role in Drosophila development. The mammalian homolog, Odd-skipped related 2 (Osr2), regulates limb, tooth, and kidney development in mouse embryos. However, the detailed expression pattern of Osr2 during neonatal development remains unclear. In this study, we investigated Osr2 expression patterns in mouse neonatal and embryo tissues using qPCR and in situ hybridization methods. First, we examined the tissue distribution of Osr2 by qPCR, and found it to be highly expressed in the uterus and moderately in the testes, small intestine, and prostate. That expression was also found in eye, kidney, placenta, lung, thymus, lymph node, stomach, and skeletal muscle tissues, and in all embryonic stages. On the other hand, Osr2 was not expressed in brain, heart, liver, or spleen samples. Next, we examined the tissue localization of Osr2 using in situ hybridization. Osr2 was found in the craniofacial region on E13.5, with notable expression in dental germ mesenchyme as well as the renal corpuscle on E17.5. As for neonatal tissues, Osr2 was expressed in the dental papilla, dental follicle, Harderian gland, nasal bone, eyelid dermis, synovial joint, and tibial subcutis. Our findings suggest that Osr2 functions in reproductive system organs, such as the uterus, testes, prostate, placenta, and ovaries. Furthermore, based on its expression in kidney, Harderian gland, eyelid dermis, and tibial subcutis tissues, this transcription factor may be involved in hormone synthesis and function. Together, our results demonstrate the role of Osr2 in postnatal development and embryogenesis.
authors.affiliation: Challenge to Intractable Oral Diseases, Centers for Frontier Oral Science and Translational Dental Research, Osaka University Graduate School of Dentistry, 1-8 Yamadaoka, Suita-Osaka 565-0871, Japan. Electronic address: skawai@dent.osaka-u.ac.jp.
authors.forename: Shinji
authors.initials: S
authors.lastname: Kawai
authors.forename: Ikumi
authors.initials: I
authors.lastname: Michikami
authors.forename: Jirouta
authors.initials: J
authors.lastname: Kitagaki
authors.forename: Ei
authors.initials: E
authors.lastname: Hashino
authors.forename: Atsuo
authors.initials: A
authors.lastname: Amano
mesh_terms: D000818:Animals; D001842:Bone and Bones / Q000378:metabolism; D003771:Dental Papilla / Q000378:metabolism; D047108:Embryonic Development; D005260:Female; D015870:Gene Expression; D018507:Gene Expression Regulation, Developmental; D007421:Intestine, Small / Q000378:metabolism; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D009297:Nasal Mucosa / Q000378:metabolism; D009928:Organ Specificity; D010920:Placenta / Q000378:metabolism; D011247:Pregnancy; D011467:Prostate / Q000378:metabolism; D013737:Testis / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D014599:Uterus / Q000378:metabolism
chemical_list: C438618:Osr2 protein, mouse; D014157:Transcription Factors
keywords: Dental papilla; Eyelid; Harderian gland; Nasal bone; Odd-skipped related 2 (Osr2); Perichondrium; Renal corpuscle; Subcutis