title: Staphylococcal cassette chromosome mec characterization of methicillin-resistant Staphylococcus aureus strains isolated at the military hospital of Constantine/Algeria.
abstract: Staphylococcal cassette chromosome mec is a genetic mobile element that carries the gene mecA mediating the methicillin resistance in staphylococci. The aim of this study is to type the Staphylococcal cassette chromosome mec (SCCmec) in 64 non-redundant methicillin-resistant Staphylococcus aureus (MRSA) strains recovered at the military hospital of Constantine (Algeria) between 2005 and 2007. Methicillin resistance was detected by oxacillin and cefoxitin discs and PBP2a test, and then confirmed by mecA PCR. The SCCmec complex types were determined by real time PCR. The analysis showed that 50 isolates were hospital acquired (HA-MRSA) and 14 were community-acquired (CA-MRSA). SCCmec type IV and V (traditionally attributed to CA-MRSA) were harbored by both HA-MRSA and CA-MRSA, while SCCmec type I, II and III were not recorded. These findings motivate more investigations to be carried on HA-MRSA in our hospital and other national health care centers.
authors.affiliation: Unité de microbiologie, hôpital militaire universitaire de Constantine, Nouvelle ville Ali Mendjeli, Constantine, Algeria. Electronic address: z_ouche@yahoo.fr.
authors.forename: Z
authors.initials: Z
authors.lastname: Ouchenane
authors.forename: A
authors.initials: A
authors.lastname: Agabou
authors.forename: F
authors.initials: F
authors.lastname: Smati
authors.forename: J-M
authors.initials: JM
authors.lastname: Rolain
authors.forename: D
authors.initials: D
authors.lastname: Raoult
mesh_terms: D000462:Algeria; D001426:Bacterial Proteins / Q000235:genetics*; D015373:Bacterial Typing Techniques; D002876:Chromosomes, Bacterial; D003001:Cloning, Molecular; D017714:Community-Acquired Infections / Q000382:microbiology*; D003428:Cross Infection / Q000382:microbiology*; D006772:Hospitals, Military; D006801:Humans; D016106:Methicillin Resistance / Q000235:genetics*; D055624:Methicillin-Resistant Staphylococcus aureus / Q000235:genetics* / Q000302:isolation & purification; D008826:Microbial Sensitivity Tests; D046915:Penicillin-Binding Proteins
chemical_list: D001426:Bacterial Proteins; D046915:Penicillin-Binding Proteins; C487789:mecA protein, Staphylococcus aureus
keywords: Algeria; Algérie; MRSA; SARM; SCCmec; Staphylococcus aureus