title: Association of the CagA gene positive Helicobacter pylori and tissue levels of interleukin-17 and interleukin-8 in gastric ulcer patients.
abstract: It has been reported that CagA gene positive Helicobacter pylori (CagA+ H. pylon) induces severe gastric mucosal inflammation. On the other hand, Interleukin (IL)-17 is known to stimulate IL-8 release by the gastric epithelial cells which facilitates chemotaxis of neutrophils through an IL-8-dependent mechanism. The aim of the study is to determine the role of IL-17 and IL-8 in the development of gastritis and gastric ulcer in H. pylori infected patients. Mucosal biopsy samples were obtained from the ulcer site of gastric mucosa of 28 patients with gastric ulcer (GU), 27 with gastritis and 8 controls subjects without gastritis or ulcers. Infection with H. pylori of patients and controls was assessed by a rapid urease test, histological examination and culture. Measurement of the tissue levels of IL-17 and IL-8 were assayed by ELISA. H. pylori cagA gene was assessed by polymerase chain reaction (PCR). Out of the 28 patients with GU, 18 (64.2%) patients were positive for H. pylori infection, while 13 (48.1%) patients with gastritis and none of the controls were positive for H. pylori infection The CagA gene was detected in 12 (66.6%) in H. pylori GU patients, and 7 (53.8%) H. pylori positive gastritis. IL-17 was significantly higher in GU-CagA+ve H. pylori compared to GU-CagA- H. pylori (P <0.05), while IL-8 showed no significant difference between groups. The mean levels of IL-8 in gastritis-CagA+ H. pylori) was significantly higher compared to gastritis--CagA- H. pylori- (P <0.05). IL-17 showed significant association with the number of neutrophils in both GU and gastritis (r = 0.689, P < 0.05 & r = 0.618, P < 0.05). Also, IL-8 showed significant association with the number of neutrophils in both GU and gastritis n (r = 0.468, P < 0.05 & r = 0.727, P < 0.05). It is concluded that the Cag+ve H. pylori is associated with induction of mucosal injury. Also, IL-8 and IL-17 plays a role in the development of GU and gastritis especially in CagA+ H. pylori.
authors.affiliation: Departments of Internal Medicine, Faculty of Medicine, Mansoura University, Mansoura, Egypt.
authors.forename: Ashraf A
authors.initials: AA
authors.lastname: El-Fakhry
authors.forename: Medhat A
authors.initials: MA
authors.lastname: El-Daker
authors.forename: Rawia I
authors.initials: RI
authors.lastname: Badr
authors.forename: Ghada M
authors.initials: GM
authors.lastname: El-Nady
authors.forename: Maggie R
authors.initials: MR
authors.lastname: Mesbah
authors.forename: Tamer
authors.initials: T
authors.lastname: Youssef
authors.forename: Mohammad
authors.initials: M
authors.lastname: Arafa
authors.forename: Mona
authors.initials: M
authors.lastname: Arafa
authors.forename: Mohammed M
authors.initials: MM
authors.lastname: El-Naggar
mesh_terms: D000942:Antigens, Bacterial / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics*; D004797:Enzyme-Linked Immunosorbent Assay; D005753:Gastric Mucosa / Q000737:chemistry / Q000276:immunology / Q000382:microbiology; D016481:Helicobacter Infections / Q000150:complications* / Q000235:genetics / Q000276:immunology; D016480:Helicobacter pylori / Q000235:genetics; D006801:Humans; D015851:Interleukin-7 / Q000032:analysis / Q000096:biosynthesis* / Q000276:immunology; D016209:Interleukin-8 / Q000032:analysis / Q000096:biosynthesis* / Q000276:immunology; D016133:Polymerase Chain Reaction; D013276:Stomach Ulcer / Q000276:immunology / Q000382:microbiology*
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D015851:Interleukin-7; D016209:Interleukin-8; C080866:cagA protein, Helicobacter pylori