title: Transcriptional analysis through RNA sequencing of giant cells induced by Meloidogyne graminicola in rice roots.
abstract: One of the reasons for the progressive yield decline observed in aerobic rice production is the rapid build-up of populations of the rice root knot nematode Meloidogyne graminicola. These nematodes induce specialized feeding cells inside root tissue, called giant cells. By injecting effectors in and sipping metabolites out of these cells, they reprogramme normal cell development and deprive the plant of its nutrients. In this research we have studied the transcriptome of giant cells in rice, after isolation of these cells by laser-capture microdissection. The expression profiles revealed a general induction of primary metabolism inside the giant cells. Although the roots were shielded from light induction, we detected a remarkable induction of genes involved in chloroplast biogenesis and tetrapyrrole synthesis. The presence of chloroplast-like structures inside these dark-grown cells was confirmed by confocal microscopy. On the other hand, genes involved in secondary metabolism and more specifically, the majority of defence-related genes were strongly suppressed in the giant cells. In addition, significant induction of transcripts involved in epigenetic processes was detected inside these cells 7 days after infection.
authors.affiliation: Department of Molecular Biotechnology, Ghent University, Coupure Links 653, B-9000, Ghent, Belgium.
authors.forename: Hongli
authors.initials: H
authors.lastname: Ji
authors.forename: Godelieve
authors.initials: G
authors.lastname: Gheysen
authors.forename: Simon
authors.initials: S
authors.lastname: Denil
authors.forename: Keith
authors.initials: K
authors.lastname: Lindsey
authors.forename: Jennifer F
authors.initials: JF
authors.lastname: Topping
authors.forename: Kamrun
authors.initials: K
authors.lastname: Nahar
authors.forename: Annelies
authors.initials: A
authors.lastname: Haegeman
authors.forename: Winnok H
authors.initials: WH
authors.lastname: De Vos
authors.forename: Geert
authors.initials: G
authors.lastname: Trooskens
authors.forename: Wim
authors.initials: W
authors.lastname: Van Criekinge
authors.forename: Tim
authors.initials: T
authors.lastname: De Meyer
authors.forename: Tina
authors.initials: T
authors.lastname: Kyndt
mesh_terms: D000818:Animals; D006706:Homeostasis; D061346:Laser Capture Microdissection; D018613:Microscopy, Confocal; D008969:Molecular Sequence Data; D012275:Oryza / Q000166:cytology / Q000235:genetics* / Q000254:growth & development / Q000469:parasitology*; D059828:Plant Cells / Q000378:metabolism; D010937:Plant Growth Regulators / Q000378:metabolism; D018517:Plant Roots / Q000166:cytology / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017423:Sequence Analysis, RNA; D059467:Transcriptome; D014431:Tylenchoidea / Q000502:physiology*
chemical_list: D010937:Plant Growth Regulators; D012333:RNA, Messenger
keywords: Giant cell; Meloidogyne graminicola; Oryza sativa; laser-capture microdissection; root knot nematode; transcriptome.