title: Screening α-glucosidase inhibitor from natural products by capillary electrophoresis with immobilised enzyme onto polymer monolith modified by gold nanoparticles.
abstract: A novel strategy for screening α-glucosidase inhibitors (AGIs) from natural products by capillary electrophoresis (CE) with an immobilised enzyme microreactor was developed. In this approach, gold nanoparticles (AuNPs) was first covalently attached to surface of the pores of the porous polymer capillary monolith via the formation of an Au-S bond, and α-glucosidase was then simply and stably immobilised onto AuNPs through the strong affinity of gold for amino groups of the enzyme. In order to profiling the activity of the immobilised α-glucosidase, the natural substrate was hydrolyzed by it and the yield of product was determined by CE. The amount of covalently attached α-glucosidase to the monolith was calculated to be about 30.0 μg/mg. The immobilised enzyme exhibited 80% activity after 25 runs, and only lost 7.6% of activity after 6 runs within 31 days. Screening of AGIs present in extracts of natural products by the proposed method was demonstrated.
authors.affiliation: Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources (Ministry of Education of China), College of Chemistry & Chemical Engineering of Guangxi Normal University, Guilin 541004, PR China.
authors.forename: Aizhu
authors.initials: A
authors.lastname: Zhang
authors.forename: Fanggui
authors.initials: F
authors.lastname: Ye
authors.forename: Junyu
authors.initials: J
authors.lastname: Lu
authors.forename: Shulin
authors.initials: S
authors.lastname: Zhao
mesh_terms: D001688:Biological Products / Q000737:chemistry*; D004353:Drug Evaluation, Preclinical / Q000295:instrumentation / Q000379:methods*; D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods*; D004791:Enzyme Inhibitors / Q000737:chemistry*; D004800:Enzymes, Immobilized / Q000737:chemistry; D065089:Glycoside Hydrolase Inhibitors; D006046:Gold / Q000737:chemistry; D007700:Kinetics; D010936:Plant Extracts / Q000737:chemistry*; D010944:Plants / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry; D000520:alpha-Glucosidases / Q000737:chemistry
chemical_list: D001688:Biological Products; D004791:Enzyme Inhibitors; D004800:Enzymes, Immobilized; D065089:Glycoside Hydrolase Inhibitors; D010936:Plant Extracts; D011108:Polymers; D006046:Gold; D000520:alpha-Glucosidases
keywords: Gold nanoparticles; Immobilised enzyme; Monolith; Natural products; α-Glucosidase inhibitor