title: Sensitive competitive immunoassay of multiple mycotoxins with non-fouling antigen microarray.
abstract: Various mycotoxins with strong carcinogenesis and toxicity are fatal threats in food safety, and require highly sensitive and high-throughput detections greatly. Herein a fluorescent competitive immunoassay microarray based on a non-fouling polymer brush, poly[(ethylene glycol) methacrylate-co-glycidyl methacrylate] (POEGMA-co-GMA) is explored to sensitively detect multiple mycotoxins with aflatoxin B1 (AFB1), ochratoxin A (OTA) and zearalenone (ZEN) as template targets. Due to uniformly large protein loading and high resistance to nonspecific protein absorption of the POEGMA-co-GMA brush, the optimal microarray exhibits wide dynamic ranges of three orders of magnitudes and low detection limits of 4, 4 and 3 pg mL(-1), respectively, which is much better than that obtained with an epoxy-functionalized antigen microarray, and is comparable or even better than the conventional ELISA method. This work offers a powerful high-throughput tool to fast screening of toxins in food quality and environmental monitoring.
authors.affiliation: Institute for Clean Energy & Advanced Materials, Southwest University, Chongqing 400715, PR China; Chongqing Key Laboratory for Advanced Materials and Technologies of Clean Energies, Chongqing 400715, PR China.
authors.forename: Weihua
authors.initials: W
authors.lastname: Hu
authors.forename: Xin
authors.initials: X
authors.lastname: Li
authors.forename: Guangli
authors.initials: G
authors.lastname: He
authors.forename: Zhaowei
authors.initials: Z
authors.lastname: Zhang
authors.forename: Xinting
authors.initials: X
authors.lastname: Zheng
authors.forename: Peiwu
authors.initials: P
authors.lastname: Li
authors.forename: Chang Ming
authors.initials: CM
authors.lastname: Li
mesh_terms: D016604:Aflatoxin B1 / Q000032:analysis; D000818:Animals; D015374:Biosensing Techniques / Q000295:instrumentation*; D002273:Carcinogens / Q000032:analysis*; D007118:Immunoassay / Q000295:instrumentation; D057230:Limit of Detection; D008689:Methacrylates / Q000737:chemistry; D009183:Mycotoxins / Q000032:analysis*; D009793:Ochratoxins / Q000032:analysis; D011092:Polyethylene Glycols / Q000737:chemistry; D040081:Protein Array Analysis; D012710:Serum Albumin, Bovine / Q000737:chemistry; D015025:Zearalenone / Q000032:analysis
chemical_list: D002273:Carcinogens; D008689:Methacrylates; D009183:Mycotoxins; D009793:Ochratoxins; C524499:polyethylene glycol methacrylate; C025589:ochratoxin A; D012710:Serum Albumin, Bovine; D011092:Polyethylene Glycols; D015025:Zearalenone; D016604:Aflatoxin B1
keywords: Antigen microarray; Competitive immunoassay; Mycotoxins; Polymer brush