title: In utero exposure to nanosized carbon black (Printex90) does not induce tandem repeat mutations in female murine germ cells.
abstract: Inhalation of particles has been shown to induce mutations in the male germline in mice following both prenatal and adult exposures in several experiments. In contrast, the effects of particles on female germ cell mutagenesis are not well established. Germline mutations are induced during active cell division, which occurs during fetal development in females. We investigated the effects of prenatal exposure to carbon black nanoparticles (CB) on induction of mutations in the female mouse germline during fetal development, spanning the critical developmental stages of oogenesis. Pregnant C57BL/6J mice were exposed four times during gestation by intratracheal instillation of 67μg/animal of nanosized carbon black Printex90 or vehicle (gestation days 7, 10, 15 and 18). Female offspring were raised to maturity and mated with unexposed CBA males. Expanded simple tandem repeat (ESTR) germline mutation rates in the resulting F2 generation were determined from full pedigrees (mother, father, offspring) of F1 female mice (178 CB-exposed and 258 control F2 offspring). ESTR mutation rates in CB-exposed F2 female offspring were not statistically different from those of F2 female control offspring.
authors.affiliation: National Research Centre for the Working Environment, DK-2100 Copenhagen, Denmark. amb@nrcwe.dk
authors.forename: Anne Mette Zenner
authors.initials: AM
authors.lastname: Boisen
authors.forename: Thomas
authors.initials: T
authors.lastname: Shipley
authors.forename: Petra
authors.initials: P
authors.lastname: Jackson
authors.forename: Håkan
authors.initials: H
authors.lastname: Wallin
authors.forename: Christine
authors.initials: C
authors.lastname: Nellemann
authors.forename: Ulla
authors.initials: U
authors.lastname: Vogel
authors.forename: Carole L
authors.initials: CL
authors.lastname: Yauk
authors.forename: Karin Sørig
authors.initials: KS
authors.lastname: Hougaard
mesh_terms: D000818:Animals; D005260:Female; D005854:Germ Cells / Q000187:drug effects; D018095:Germ-Line Mutation; D008431:Maternal-Fetal Exchange; D051379:Mice; D008810:Mice, Inbred C57BL; D053758:Nanoparticles / Q000633:toxicity*; D011247:Pregnancy; D053260:Soot / Q000633:toxicity*; D020080:Tandem Repeat Sequences
chemical_list: D053260:Soot
keywords: CB; Carbon black; ESTR; F1; F2; GD; Germ cells; Germ line mutagens; Nanoparticles; Oogenesis; P; PND; Prenatal; ROS; carbon black Printex90; expanded simple tandem repeat; first generation; gestation day; parental generation; postnatal day; reactive oxygen species; second generation