title: Possible role of the Nt-4/1 protein in macromolecular transport in vascular tissue.
abstract: The Arabidopsis thaliana 4/1 (At-4/1) protein has a highly α-helical structure with potential to interact both with itself and other protein ligands, including the movement proteins of some plant viruses; the Nicotiana tabacum ortholog (Nt-4/1) has similar structure. Here we describe localization of GUS expression in transgenic N. tabacum seedlings under control of the Nt-4/1 promoter, which indicates that transcription is associated with the veins at certain developmental stages, and especially in the hypocotyl. Viroid accumulation and movement was altered in plants in which 4/1 expression was reduced by virus-induced gene silencing. These localization studies support a role of 4/1 in signaling in the vasculature,including mobility of pathogen-related and cellular RNAs.
authors.forename: Andrey G
authors.initials: AG
authors.lastname: Solovyev
authors.forename: Svetlana S
authors.initials: SS
authors.lastname: Makarova
authors.forename: Margarita V
authors.initials: MV
authors.lastname: Remizowa
authors.forename: Hyoun-Sub
authors.initials: HS
authors.lastname: Lim
authors.forename: John
authors.initials: J
authors.lastname: Hammond
authors.forename: Robert A
authors.initials: RA
authors.lastname: Owens
authors.forename: Lilya
authors.initials: L
authors.lastname: Kopertekh
authors.forename: Joachim
authors.initials: J
authors.lastname: Schiemann
authors.forename: Sergey Y
authors.initials: SY
authors.lastname: Morozov
mesh_terms: D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D001692:Biological Transport / Q000235:genetics / Q000502:physiology; D018506:Gene Expression Regulation, Plant; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D014026:Nicotiana / Q000235:genetics / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; C514342:At-4-1 protein, Arabidopsis; D010940:Plant Proteins