title: One-pot squaric acid diester mediated aqueous protein conjugation.
abstract: A water-soluble squaric acid dialkyl diester derivative is presented, which enables one-pot, two-step amine-selective protein conjugation reactions with (functional) amines in water. This reagent not only allows all-aqueous protein modifications, but also tolerates e.g. hydroxyl groups and can also be used for the modification of proteins with water-insoluble amines.
authors.affiliation: École Polytechnique Fédérale de Lausanne (EPFL), Institut des Matériaux and Institut des Sciences et Ingénierie Chimiques, Laboratoire des Polymères, Bâtiment MXD, Station 12, CH-1015 Lausanne, Switzerland.
authors.forename: Frederik
authors.initials: F
authors.lastname: Wurm
authors.forename: Tobias
authors.initials: T
authors.lastname: Steinbach
authors.forename: Harm-Anton
authors.initials: HA
authors.lastname: Klok
mesh_terms: D000588:Amines / Q000737:chemistry; D000818:Animals; D002417:Cattle; D003503:Cyclobutanes / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000378:metabolism; D014867:Water / Q000737:chemistry
chemical_list: D000588:Amines; D003503:Cyclobutanes; D011506:Proteins; D014867:Water; D012710:Serum Albumin, Bovine; C030042:squaric acid