title: Whey protein inhibits iron overload-induced oxidative stress in rats.
abstract: In this study, we evaluated the effects of whey protein on oxidative stress in rats that were subjected to oxidative stress induced by iron overload. Thirty male rats were assigned to 3 groups: the control group (regular [50 mg/kg diet] dose of iron+20% casein), iron overload group (high [2,000 mg/kg] dose of iron+20% casein, IO), and whey protein group (high dose of iron+10% casein+10% whey protein, IO+whey). After 6 wk, the IO group showed a reduction in the plasma total radical trapping antioxidant parameter and the activity of erythrocyte superoxide dismutase and an increase in lipid peroxidation (determined from the proportion of conjugated dienes). However, whey protein ameliorated the oxidative changes induced by iron overload. The concentration of erythrocyte glutathione was significantly higher in the IO+whey group than in the IO group. In addition, whey protein supplementation fully inhibited iron overload-induced DNA damage in leukocytes and colonocytes. A highly significant positive correlation was observed between plasma iron levels and DNA damage in leukocytes and colonocytes. These results show the antioxidative and antigenotoxic effects of whey protein in an in vivo model of iron overload-induced oxidative stress.
authors.affiliation: Department of Food and Nutrition, Kyungnam University, Changwon 631-701, Korea.
authors.forename: Jungmi
authors.initials: J
authors.lastname: Kim
authors.forename: Hyun-Dong
authors.initials: HD
authors.lastname: Paik
authors.forename: Yoh-Chang
authors.initials: YC
authors.lastname: Yoon
authors.forename: Eunju
authors.initials: E
authors.lastname: Park
mesh_terms: D000818:Animals; D016587:Antimutagenic Agents / Q000494:pharmacology / Q000627:therapeutic use; D000975:Antioxidants / Q000378:metabolism / Q000494:pharmacology / Q000627:therapeutic use*; D003106:Colon / Q000166:cytology / Q000187:drug effects; D004249:DNA Damage / Q000187:drug effects*; D004032:Diet; D019587:Dietary Supplements; D004912:Erythrocytes / Q000378:metabolism; D005978:Glutathione / Q000097:blood; D007501:Iron / Q000008:administration & dosage / Q000009:adverse effects* / Q000097:blood; D019190:Iron Overload / Q000097:blood / Q000188:drug therapy* / Q000235:genetics; D007962:Leukocytes / Q000187:drug effects; D015227:Lipid Peroxidation / Q000187:drug effects*; D008297:Male; D008894:Milk Proteins / Q000494:pharmacology / Q000627:therapeutic use*; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000187:drug effects*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013482:Superoxide Dismutase / Q000097:blood; D000067816:Whey Proteins
chemical_list: D016587:Antimutagenic Agents; D000975:Antioxidants; D008894:Milk Proteins; D000067816:Whey Proteins; D007501:Iron; D013482:Superoxide Dismutase; D005978:Glutathione