title: Piezo1 plays a role in erythrocyte volume homeostasis.
abstract: Mechanosensitivity is an inherent property of virtually all cell types, allowing them to sense and respond to physical environmental stimuli. Stretch-activated ion channels represent a class of mechanosensitive proteins which allow cells to respond rapidly to changes in membrane tension; however their identity has remained elusive. The piezo genes have recently been identified as a family of stretch-activated mechanosensitive ion channels. We set out to determine the role of piezo1 during zebrafish development. Here we report that morpholino-mediated knockdown of piezo1 impairs erythrocyte survival without affecting hematopoiesis or differentiation. Our results demonstrate that piezo1 is involved in erythrocyte volume homeostasis, disruption of which results in swelling/lysis of red blood cells and consequent anemia.
authors.affiliation: chris.jopling@igf.cnrs.fr.
authors.forename: Adèle
authors.initials: A
authors.lastname: Faucherre
authors.forename: Karima
authors.initials: K
authors.lastname: Kissa
authors.forename: Joël
authors.initials: J
authors.lastname: Nargeot
authors.forename: Matteo E
authors.initials: ME
authors.lastname: Mangoni
authors.forename: Chris
authors.initials: C
authors.lastname: Jopling
mesh_terms: D000818:Animals; D004911:Erythrocyte Volume / Q000235:genetics*; D004920:Erythropoiesis / Q000235:genetics; D018507:Gene Expression Regulation, Developmental; D055785:Gene Knockdown Techniques; D006706:Homeostasis / Q000235:genetics*; D007473:Ion Channels / Q000235:genetics* / Q000378:metabolism; D015027:Zebrafish / Q000097:blood* / Q000196:embryology / Q000235:genetics*; D029961:Zebrafish Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: D007473:Ion Channels; C572766:Piezo1 protein, zebrafish; D029961:Zebrafish Proteins