title: Effect of eurycomanone on cytochrome P450 isoforms CYP1A2, CYP2A6, CYP2C8, CYP2C9, CYP2C19, CYP2E1 and CYP3A4 in vitro.
abstract: Eurycomanone, an active constituent isolated from Eurycoma longifolia Jack, was examined for modulatory effects on cytochrome P450 (CYP) isoforms CYP1A2, CYP2A6, CYP2C8, CYP2C9, CYP2C19, CYP2E1 and CYP3A4 using in vitro assays. The IC50 value was determined to assess the potencies of modulation for each CYP isoform. Our results indicated that eurycomanone did not potently inhibit any of the CYP isoforms investigated, with IC50 values greater than 250 μg/ml. Hence there appears to be little likelihood of drug-herb interaction between eurycomanone or herbal products with high content of this compound and CYP drug substrates via CYP inhibition.
authors.affiliation: School of Medical Sciences, International Medical University, 126 Jalan 19/155B, Bukit Jalil, 57000, Kuala Lumpur, Malaysia.
authors.forename: Yan
authors.initials: Y
authors.lastname: Pan
authors.forename: Kai Hung
authors.initials: KH
authors.lastname: Tiong
authors.forename: Badrul Amini
authors.initials: BA
authors.lastname: Abd-Rashid
authors.forename: Zakiah
authors.initials: Z
authors.lastname: Ismail
authors.forename: Rusli
authors.initials: R
authors.lastname: Ismail
authors.forename: Joon Wah
authors.initials: JW
authors.lastname: Mak
authors.forename: Chin Eng
authors.initials: CE
authors.lastname: Ong
mesh_terms: D001189:Aryl Hydrocarbon Hydroxylases / Q000378:metabolism; D019388:Cytochrome P-450 CYP1A2 / Q000378:metabolism; D065637:Cytochrome P-450 CYP2A6; D065731:Cytochrome P-450 CYP2C19; D065727:Cytochrome P-450 CYP2C8; D065729:Cytochrome P-450 CYP2C9; D019392:Cytochrome P-450 CYP2E1 / Q000378:metabolism; D051544:Cytochrome P-450 CYP3A / Q000378:metabolism; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D006801:Humans; D007527:Isoenzymes / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology*; D036702:Quassins / Q000494:pharmacology*
chemical_list: D007527:Isoenzymes; D010936:Plant Extracts; D036702:Quassins; C506425:eurycomanone; D003577:Cytochrome P-450 Enzyme System; C450260:CYP2C9 protein, human; D065729:Cytochrome P-450 CYP2C9; D019392:Cytochrome P-450 CYP2E1; D001189:Aryl Hydrocarbon Hydroxylases; C585710:CYP2A6 protein, human; C045793:CYP2C19 protein, human; C450259:CYP2C8 protein, human; D019388:Cytochrome P-450 CYP1A2; D065637:Cytochrome P-450 CYP2A6; D065731:Cytochrome P-450 CYP2C19; D065727:Cytochrome P-450 CYP2C8; D051544:Cytochrome P-450 CYP3A; C510163:CYP3A4 protein, human