title: EDA-containing fibronectin levels in the cerebrospinal fluid of children with meningitis.
abstract: Fibronectin containing an alternatively spliced extra domain A (EDA-FN) participates in diverse biological cell functions, being also directly or indirectly engaged during an inflammatory response to brain injury and/or neuron regeneration. We analyzed FN and EDA-FN isoform levels by ELISA in 85 cerebrospinal fluid samples and 67 plasma samples obtained from children suffering from bacterial or viral meningitis and non-meningitis peripheral inflammation. We have found that the cerebrospinal level of EDA-FN was significantly lower in the bacterial meningitis group than in the viral- and non-meningitis groups. In the patients' plasma, EDA-FN was almost undetectable. The determination of fibronectin containing the EDA segment might be considered as an additional diagnostic marker of bacterial meningitis in children.
authors.affiliation: Department of Chemistry and Immunochemistry, Wrocław Medical University, ul. Bujwida 44a, 50-345 Wrocław, Poland. malgorzata.pupek@umed.wroc.pl
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Pupek
authors.forename: Jolanta
authors.initials: J
authors.lastname: Jasonek
authors.forename: Iwona
authors.initials: I
authors.lastname: Kątnik-Prastowska
mesh_terms: D000293:Adolescent; D015415:Biomarkers; D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005353:Fibronectins / Q000097:blood / Q000134:cerebrospinal fluid*; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D007249:Inflammation / Q000097:blood / Q000134:cerebrospinal fluid* / Q000473:pathology; D008297:Male; D016920:Meningitis, Bacterial / Q000097:blood / Q000134:cerebrospinal fluid* / Q000473:pathology; D008587:Meningitis, Viral / Q000097:blood / Q000134:cerebrospinal fluid* / Q000473:pathology; D011379:Prognosis; D020033:Protein Isoforms; D012372:ROC Curve
chemical_list: D015415:Biomarkers; D005353:Fibronectins; D020033:Protein Isoforms; C574442:extra domain A fibronectin, human
keywords: EDA-fibronectin isoform; bacterial and viral meningitis; cerebrospinal fluid; fibronectin