title: The glyceraldehyde-3-phosphate dehydrogenase promoter of the food yeast Candida utilis strain NRRL Y-660 is functional in Agrobacterium tumefaciens.
abstract: The glyceraldehyde-3-phosphate dehydrogenase promoter of the food yeast Candida utilis strain NRRL Y-660 was cloned to create a novel integrative vector for Agrobacterium tumefaciens-mediated transformation. The new binary vector harbors β-glucuronidase activity as reporter and kanamicin/geneticin resistance as selection marker. Recombinant clones of A. tumefaciens show kanamycin resistance and high β-glucuronidase activity under the control of the C. utilis promoter. This finding can be explained by the presence of a prokaryotic core in the yeast promoter, predicted by in silico analysis of the sequence. This is the first report about functionality of a yeast promoter in A. tumefaciens.
authors.affiliation: Biotechnology Department, Cuban Research Institute on Sugarcane By-Products (ICIDCA), Vía Blanca 804 & Carretera Central, San Miguel del Padrón, Havana, Cuba, POBox 4026, tania.gonzalez@icidca.edu.cu.
authors.forename: Tania
authors.initials: T
authors.lastname: González
authors.forename: Felipe
authors.initials: F
authors.lastname: Eng
authors.forename: Reinaldo
authors.initials: R
authors.lastname: Fraga
authors.forename: Jennifer
authors.initials: J
authors.lastname: Fonseca
authors.forename: Isis
authors.initials: I
authors.lastname: Amores
mesh_terms: D016960:Agrobacterium tumefaciens / Q000235:genetics* / Q000378:metabolism; D002175:Candida / Q000201:enzymology / Q000235:genetics*; D003001:Cloning, Molecular; D004271:DNA, Fungal / Q000235:genetics; D005822:Genetic Vectors; D005839:Gentamicins; D005966:Glucuronidase / Q000235:genetics / Q000378:metabolism; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000235:genetics*; D007612:Kanamycin; D011401:Promoter Regions, Genetic; D014170:Transformation, Genetic
chemical_list: D004271:DNA, Fungal; D005839:Gentamicins; D007612:Kanamycin; C010680:antibiotic G 418; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; D005966:Glucuronidase