title: The effect of chlorpyrifos on isolated thoracic aorta in rats.
abstract: This study investigated the effect of chlorpyrifos on thoracic aorta and on the level of NO in plasma and aorta. The effect of chlorpyrifos on thoracic aorta in organ bath was determined in 10 rats. Another 45 rats were assigned to 3 groups with 15 rats each: control group 1 received distilled water, control group 2 was given corn oil, and the last group was given 13.5 mg/kg chlorpyrifos dissolved in corn oil every other day for 8 weeks orally. Chlorpyrifos (10(-10) M-10(-5) M) showed no effect on isolated thoracic aorta. Plasma AChE activity was decreased, while LDH, ALT, GGT, and AST activities were increased in chlorpyrifos group compared to control groups. Plasma NO level was increased in chlorpyrifos group compared to control groups. iNOS expression was present in all groups in the cytoplasm of the endothelia and in the smooth muscle cells of aorta. According to semiquantitative histomorphological analysis, iNOS immunopositive reactions were seen in the decreasing order in chlorpyrifos, control 2, and control 1 groups. eNOS immunopositive reactions were observed in the endothelial cell cytoplasm, rarely in the subintimal layer, and the smooth muscle cells of aorta. There were no differences among the groups in terms of eNOS immunostaining. In conclusion, chlorpyrifos induced NO production in aorta following an increase in NOS expression.
authors.affiliation: Pharmacology and Toxicology Department, Veterinary Faculty, Kırıkkale University, Yahşihan, 71451 Kırıkkale, Turkey. ebru32yildirim@gmail.com
authors.forename: Ebru
authors.initials: E
authors.lastname: Yıldırım
authors.forename: Emine
authors.initials: E
authors.lastname: Baydan
authors.forename: Murat
authors.initials: M
authors.lastname: Kanbur
authors.forename: Oğuz
authors.initials: O
authors.lastname: Kul
authors.forename: Miyase
authors.initials: M
authors.lastname: Cınar
authors.forename: Hüsamettin
authors.initials: H
authors.lastname: Ekici
authors.forename: Nurgül
authors.initials: N
authors.lastname: Atmaca
mesh_terms: D000818:Animals; D001013:Aorta, Thoracic / Q000187:drug effects* / Q000378:metabolism*; D004390:Chlorpyrifos / Q000008:administration & dosage*; D002800:Cholinesterase Inhibitors / Q000008:administration & dosage; D004305:Dose-Response Relationship, Drug; D008297:Male; D009569:Nitric Oxide / Q000096:biosynthesis*; D019001:Nitric Oxide Synthase / Q000096:biosynthesis*; D009924:Organ Culture Techniques; D010575:Pesticides / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar
chemical_list: D002800:Cholinesterase Inhibitors; D010575:Pesticides; D009569:Nitric Oxide; D019001:Nitric Oxide Synthase; D004390:Chlorpyrifos