title: Evaluation of Anticancer Activity of Fruit and Leave Extracts from Virus Infected and Healthy Cultivars of Vitis vinifera.
abstract: OBJECTIVE
  Grape virus diseases are a serious problem in Iran. Leaves and fruits of grape have been used for different purposes like cooking in Iran. The present investigation was carried out to study on the cytotoxic-activities of extracts of fruits and leaves of Vitis vinifera from both virus-free and virus-infected grape cultivars against breast cancer cell line (MDAMB- 231) and human embryonic kidney normal cell line (HEK 293).
  
  
  METHODS
  IN THIS EXPERIMENTAL STUDY, THE CONSIDERED GRAPE CULTIVARS WERE AS FOLLOWS: Rish Baba Sefid, Shahani Ghasre Shirin, Rotabi Zarghan, Asgari Najaf Abad, Fars, Kaj Angor Bojnord, Sarkesh Shiraz and Siahe Zarqan. A real-time multiplex polymerase chain reaction (real-time Multiplex PCR) assay was applied to detect virus infected cultivars. The cytotoxic effect of the methanol extracts of different Vitis vinifera varieties on cultured cells was monitored using (3- (4, 5-Dimethylthiazol-2-yl) -2, 5-diphenyltetrazolium bromide (MTT) assay at different concentrations (62.5, 125, 250, 500, 750, 1000 μg mL(-1)).
  
  
  RESULTS
  Among these cultivars, Grapevine fanleaf virus (GFLV) along with related symptoms was detected in Siahe Zarqan and Fars. Methanolic extracts of leaves and fruits of Vitis vinifera from both virus free and virus infected cultivars showed a range of limited to moderate cytotoxic activity. However, methanol extract of leaves belonged to virus infected cultivars was found to have strong cytotoxic effect against MDA-MB-231 at different concentrations.
  
  
  CONCLUSIONS
  Grapevine fanleaf virus (GFLV) can potentially increase the cytotoxicity of grape cultivars.
authors.affiliation: 1. Department of Biotechnology, Faculty of Advanced Sciences and Technologies, University of Isfahan, Isfahan, Iran.
authors.forename: Zahra
authors.initials: Z
authors.lastname: Esfahanian
authors.forename: Mandana
authors.initials: M
authors.lastname: Behbahani
authors.forename: Mehrnaz
authors.initials: M
authors.lastname: Shanehsaz
authors.forename: Mohammad Javad
authors.initials: MJ
authors.lastname: Hessami
authors.forename: Mohammad Ali
authors.initials: MA
authors.lastname: Nejatian
keywords: Anticancer; Virus; Vitis vinifera