title: Rapid solid-phase extraction and analysis of resveratrol and other polyphenols in red wine.
abstract: Red wine has long been credited as a good source of health-beneficial antioxidants, including the bioactive polyphenols catechin, quercetin, and (E)-resveratrol. In this paper, we report the application of reusable molecularly imprinted polymers (MIPs) for the selective and robust solid-phase extraction (SPE) and rapid analysis of (E)-resveratrol (LOD=8.87×10(-3) mg/L, LOQ=2.94×10(-2) mg/L), along with a range of other polyphenols from an Australian Pinot noir red wine. Optimization of the molecularly imprinted solid-phase extraction (MISPE) protocol resulted in the significant enrichment of (E)-resveratrol and several structurally related polyphenols. These secondary metabolites were subsequently identified by RP-HPLC and μLC-ESI ion trap MS/MS methods. The developed MISPE protocol employed low volumes of environmentally benign solvents selected according to the Green Chemistry principles, and resulted in the recovery of 99% of the total (E)-resveratrol present. These results further demonstrate the potential of generic protocols for the analysis of target compound with health beneficial properties within the food and nutraceutical industries using tailor-made MIPs.
authors.affiliation: Centre for Green Chemistry, Monash University, Monash, 3800 Victoria, Australia.
authors.forename: Shima N N S
authors.initials: SN
authors.lastname: Hashim
authors.forename: Lachlan J
authors.initials: LJ
authors.lastname: Schwarz
authors.forename: Reinhard I
authors.initials: RI
authors.lastname: Boysen
authors.forename: Yuanzhong
authors.initials: Y
authors.lastname: Yang
authors.forename: Basil
authors.initials: B
authors.lastname: Danylec
authors.forename: Milton T W
authors.initials: MT
authors.lastname: Hearn
mesh_terms: D002851:Chromatography, High Pressure Liquid; D057230:Limit of Detection; D054802:Molecular Imprinting; D059808:Polyphenols / Q000032:analysis / Q000737:chemistry* / Q000302:isolation & purification; D000077185:Resveratrol; D052616:Solid Phase Extraction / Q000379:methods*; D021241:Spectrometry, Mass, Electrospray Ionization; D013267:Stilbenes / Q000032:analysis / Q000737:chemistry* / Q000302:isolation & purification; D053719:Tandem Mass Spectrometry; D014920:Wine / Q000032:analysis*
chemical_list: D059808:Polyphenols; D013267:Stilbenes; D000077185:Resveratrol
keywords: (E)-Resveratrol; Capillary HPLC–ESI ion trap MS/MS; Molecularly imprinted polymer; Pinot noir red wine; Solid-phase extraction