title: Morphological and ribosomal DNA-based characterization of six Antarctic ciliate morphospecies from the Amundsen Sea with phylogenetic analyses.
abstract: We characterized six tintinnid ciliates from Antarctic waters using molecular markers and morphological traits: Amphorellopsis quinquealata, Codonellopsis gaussi, Cymatocylis convallaria, Cy. calyciformis, Cy. drygalskii, and Laackmanniella prolongata. The 100% similarity in SSU-ITS1-5.8S rDNA-ITS2-partial LSU rDNA sequences among Cy. convallaria, Cy. calyciformis, and Cy. drygalskii is supportive of synonymy. Codonellopsis gaussi and L. prolongata also showed high levels of similarity in SSU rDNA (99.83%) and the D2 domain of LSU rDNA (95.77%), suggesting that they are closely related. Phylogenetic analysis placed Cymatocylis in the Rhabdonellidae, Amphorellopsis in the Tintinnidae and L. prolongata/Co. gaussi within the Dictyocystidae.
authors.affiliation: Department of Oceanography, Inha University, Incheon, 402-751, Korea; Korea Polar Research Institute, KIOST, 213-3 Sondo-dong, Yeonsu-gu, Incheon, 405-840, Korea.
authors.forename: Sun Young
authors.initials: SY
authors.lastname: Kim
authors.forename: Joong Ki
authors.initials: JK
authors.lastname: Choi
authors.forename: John R
authors.initials: JR
authors.lastname: Dolan
authors.forename: Hyoung Chul
authors.initials: HC
authors.lastname: Shin
authors.forename: SangHoon
authors.initials: S
authors.lastname: Lee
authors.forename: Eun Jin
authors.initials: EJ
authors.lastname: Yang
mesh_terms: D000864:Antarctic Regions; D016798:Ciliophora / Q000145:classification* / Q000166:cytology / Q000235:genetics / Q000302:isolation & purification*; D016000:Cluster Analysis; D016054:DNA, Protozoan / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics; D020459:Genes, rRNA; D008853:Microscopy; D008969:Molecular Sequence Data; D010802:Phylogeny; D016053:RNA, Protozoan / Q000235:genetics; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D012340:RNA, Ribosomal, 5.8S / Q000235:genetics; D012623:Seawater / Q000469:parasitology*; D017422:Sequence Analysis, DNA
chemical_list: D016054:DNA, Protozoan; D004275:DNA, Ribosomal; D021903:DNA, Ribosomal Spacer; D016053:RNA, Protozoan; D012337:RNA, Ribosomal, 18S; D012340:RNA, Ribosomal, 5.8S
keywords: 18S; 28S; Antarctica; Tintinnida; marine ciliates; planktonic ciliates; taxonomy