title: Decreased microRNA-221 is associated with high levels of TNF-α in human adipose tissue-derived mesenchymal stem cells from obese woman.
abstract: OBJECTIVE
  The present study aimed to investigate the regulation and involvement of miR-221 in the differentiation of human adipose tissue-derived mesenchymal stem cells (hASCs). The relationships between miR-221 and pro-inflammatory markers and adipokines were also explored.
  
  
  METHODS
  Eight adipose tissues were obtained from four obese (mean body mass index (BMI) =31.7 kg/m(2)) and four lean (mean BMI= 21.5 kg/m(2)) women. hASCs were induced to differentiate, and the related gene expression were measured in the hASC-differentiated adipocytes using real-time reverse transcriptase polymerase chain reaction (real-time RT-PCR).
  
  
  RESULTS
  During adipogenesis, miR-221 was significantly down-regulated; furthermore, miR-221 levels were lower in hASC-differentiated adipocytes from obese subjects than in the corresponding adipocytes from lean subjects. Higher TNF-α mRNA levels were associated with lower levels of miR-221. In addition, the miR-221 levels in the adipocytes were inversely correlated with BMI.
  
  
  CONCLUSIONS
  Our results support the link between miR-221 and obesity development as well as obesity related inflammatory status.
authors.affiliation: Department of Medical Research, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan.
authors.forename: Wen-Wen
authors.initials: WW
authors.lastname: Chou
authors.forename: Yu-Ting
authors.initials: YT
authors.lastname: Wang
authors.forename: Yi-Chu
authors.initials: YC
authors.lastname: Liao
authors.forename: Shih-Chang
authors.initials: SC
authors.lastname: Chuang
authors.forename: Shen-Nien
authors.initials: SN
authors.lastname: Wang
authors.forename: Suh-Hang Hank
authors.initials: SH
authors.lastname: Juo
mesh_terms: D050156:Adipogenesis; D054392:Adipokines / Q000378:metabolism; D000273:Adipose Tissue / Q000166:cytology*; D000328:Adult; D016207:Cytokines / Q000378:metabolism; D015536:Down-Regulation; D005260:Female; D006801:Humans; D050356:Lipid Metabolism; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D035683:MicroRNAs / Q000378:metabolism*; D008875:Middle Aged; D009765:Obesity / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D012333:RNA, Messenger / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism*
chemical_list: D054392:Adipokines; D016207:Cytokines; D035683:MicroRNAs; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; C525709:MIR221, human