title: Flooding tolerance in interspecific introgression lines containing chromosome segments from teosinte (Zea nicaraguensis) in maize (Zea mays subsp. mays).
abstract: OBJECTIVE
  Nicaraguan teosinte (Zea nicaraguensis), a species found in frequently flooded areas, provides useful germplasm for breeding flooding-tolerant maize (Z. mays subsp. mays). The objective of this study was to select flooding-tolerant lines using a library of introgression lines (ILs), each containing a chromosome segment from Z. nicaraguensis in the maize inbred line Mi29.
  
  
  METHODS
  To produce the ILs, a single F1 plant derived from a cross between maize Mi29 and Z. nicaraguensis was backcrossed to Mi29 three times, self-pollinated four times and genotyped using simple sequence repeat markers. Flooding tolerance was evaluated at the seedling stage under reducing soil conditions.
  
  
  RESULTS
  By backcrossing and selfing, a series of 45 ILs were developed covering nearly the entire maize genome. Five flooding-tolerant lines were identified from among the ILs by evaluating leaf injury. Among these, line IL#18, containing a Z. nicaraguensis chromosome segment on the long arm of chromosome 4, showed the greatest tolerance to flooding, suggesting the presence of a major quantitative trait locus (QTL) in that region. The presence of the QTL was verified by examining flooding tolerance in a population segregating for the candidate region of chromosome 4. There was no significant relationship between the capacity to form constitutive aerenchyma and flooding tolerance in the ILs, indicating the presence of other factors related to flooding tolerance under reducing soil conditions.
  
  
  CONCLUSIONS
  A flooding-tolerant genotype, IL#18, was identified; this genotype should be useful for maize breeding. In addition, because the chromosome segments of Z. nicaraguensis in the ILs cover nearly the entire genome and Z. nicaraguensis possesses several unique traits related to flooding tolerance, the ILs should be valuable material for additional QTL detection and the development of flooding-tolerant maize lines.
authors.affiliation: Forage Crop Research Division, NARO Institute of Livestock and Grassland Science, 768 Senbonmatsu, Nasushiobara, Tochigi 329-2793, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Mano
authors.forename: F
authors.initials: F
authors.lastname: Omori
mesh_terms: D001947:Breeding; D002678:Chimera; D002874:Chromosome Mapping; D032461:Chromosomes, Plant / Q000235:genetics*; D055868:Floods; D008040:Genetic Linkage; D018745:Genome, Plant / Q000235:genetics*; D005838:Genotype; D010641:Phenotype; D018515:Plant Leaves / Q000235:genetics / Q000502:physiology; D018517:Plant Roots / Q000235:genetics / Q000502:physiology; D018520:Plant Shoots / Q000235:genetics / Q000502:physiology; D040641:Quantitative Trait Loci / Q000235:genetics*; D036226:Seedlings / Q000235:genetics / Q000502:physiology; D012987:Soil; D013312:Stress, Physiological; D003313:Zea mays / Q000235:genetics* / Q000502:physiology
chemical_list: D012987:Soil
keywords: Aerenchyma; QTL; flooding tolerance; genetic resources; introgression lines; quantitative trait loci; recombination suppression; reducing soil conditions; teosinte; waterlogging; wide hybridization