title: MutMap+: genetic mapping and mutant identification without crossing in rice.
abstract: Advances in genome sequencing technologies have enabled researchers and breeders to rapidly associate phenotypic variation to genome sequence differences. We recently took advantage of next-generation sequencing technology to develop MutMap, a method that allows rapid identification of causal nucleotide changes of rice mutants by whole genome resequencing of pooled DNA of mutant F2 progeny derived from crosses made between candidate mutants and the parental line. Here we describe MutMap+, a versatile extension of MutMap, that identifies causal mutations by comparing SNP frequencies of bulked DNA of mutant and wild-type progeny of M3 generation derived from selfing of an M2 heterozygous individual. Notably, MutMap+ does not necessitate artificial crossing between mutants and the wild-type parental line. This method is therefore suitable for identifying mutations that cause early development lethality, sterility, or generally hamper crossing. Furthermore, MutMap+ is potentially useful for gene isolation in crops that are recalcitrant to artificial crosses.
authors.affiliation: Iwate Biotechnology Research Center, Kitakami, Iwate, Japan.
authors.forename: Rym
authors.initials: R
authors.lastname: Fekih
authors.forename: Hiroki
authors.initials: H
authors.lastname: Takagi
authors.forename: Muluneh
authors.initials: M
authors.lastname: Tamiru
authors.forename: Akira
authors.initials: A
authors.lastname: Abe
authors.forename: Satoshi
authors.initials: S
authors.lastname: Natsume
authors.forename: Hiroki
authors.initials: H
authors.lastname: Yaegashi
authors.forename: Shailendra
authors.initials: S
authors.lastname: Sharma
authors.forename: Shiveta
authors.initials: S
authors.lastname: Sharma
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Kanzaki
authors.forename: Hideo
authors.initials: H
authors.lastname: Matsumura
authors.forename: Hiromasa
authors.initials: H
authors.lastname: Saitoh
authors.forename: Chikako
authors.initials: C
authors.lastname: Mitsuoka
authors.forename: Hiroe
authors.initials: H
authors.lastname: Utsushi
authors.forename: Aiko
authors.initials: A
authors.lastname: Uemura
authors.forename: Eiko
authors.initials: E
authors.lastname: Kanzaki
authors.forename: Shunichi
authors.initials: S
authors.lastname: Kosugi
authors.forename: Kentaro
authors.initials: K
authors.lastname: Yoshida
authors.forename: Liliana
authors.initials: L
authors.lastname: Cano
authors.forename: Sophien
authors.initials: S
authors.lastname: Kamoun
authors.forename: Ryohei
authors.initials: R
authors.lastname: Terauchi
mesh_terms: D000595:Amino Acid Sequence; D001947:Breeding; D002874:Chromosome Mapping / Q000379:methods*; D003433:Crosses, Genetic; D004252:DNA Mutational Analysis / Q000379:methods*; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods; D008969:Molecular Sequence Data; D009154:Mutation / Q000502:physiology; D012275:Oryza / Q000235:genetics*; D010641:Phenotype; D030821:Plants, Genetically Modified; D020641:Polymorphism, Single Nucleotide