title: Histologic effects of demineralized bone matrix on regeneration of alveolar socket in diabetic rats.
abstract: BACKGROUND
  The aim of this in vivo study was to determine the effect of demineralized bone matrix (DBM) on alveolar bone repair in type I diabetic rats.
  
  
  METHODS
  This study was carried out on 40 adult (8 weeks-old) albino rats with an average weight of 200-250 grams. The animals were divided into four groups (n=10) as follows: group 1 nondiabetic rats, group 2, 3 and 4 were diabetic rats, group 4 rats took one unit of insulin daily. Diabetes was induced by Alloxan Monohydrate through the tail veins of the rats in groups 2-4. Only group 4 received insulin NPH 1 unit daily. After 10 days, the upper right incisors of all samples were extracted and the socket was filled with DBM in groups 3 and 4. The animals were sacrificed at the end of week 1 and 2. The specimens were prepared and stained with H&E.
  
  
  RESULTS
  Histological results of group 4 displayed osteoblastic activity and bone formation with collagen fibers at the end of the first week and thick bone trabeculae formation in vicinity of DBM at the end of second week. In group 3, DBM showed some osteoinductivity at the end of the first week, but in some regions DBM particles were degraded by osteoclastic activity. Bone trabeculae formed with a dispersed and separate pattern at the end of second week. In group 2 hematoma and inflammation were dominant histological features at the end of first and second weeks; poor bone formation was detected in these two groups (2 and 3). In group 1, the results were as expected.
  
  
  CONCLUSIONS
  It seems demineralized bone matrix simulate osteoblastic activity.
authors.affiliation: Department of Periodontics, Dental School, Hamadan University of Medical Sciences, Hamadan, Iran.
authors.forename: Mahdi
authors.initials: M
authors.lastname: Kadkhodazadeh
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Mollaverdi
authors.forename: Hamid Reza
authors.initials: HR
authors.lastname: Abdolsamadi
authors.forename: Ramin
authors.initials: R
authors.lastname: Azar
authors.forename: Madjid
authors.initials: M
authors.lastname: Ghasemian Pour
authors.forename: Shahriyar
authors.initials: S
authors.lastname: Ahmadpour
keywords: Demineralized bone matrix; Diabetes; Microscopy; Tooth socket