title: Co-encapsulation of human serum albumin and superparamagnetic iron oxide in PLGA nanoparticles: part I. Effect of process variables on the mean size.
abstract: PLGA (poly d,l-lactic-co-glycolic acid) nanoparticles (NPs) encapsulating magnetite nanoparticles (MNPs) along with a model drug human serum albumin (HSA) were prepared by double emulsion solvent evaporation method. This Part I will focus on size and size distribution of prepared NPs, whereas encapsulation efficiency will be discussed in Part II. It was found that mean hydrodynamic particle size was influenced by five important process variables. To explore their effects, a five-factorial, three-level experimental design and statistical analysis were carried out using STATISTICA® software. Effect of process variables on the mean size of nanoparticles was investigated and finally conditions to minimize size of NPs were proposed. GAMS™/MINOS software was used for optimization. The mean hydrodynamic size of nanoparticles ranged from 115 to 329 nm depending on the process conditions. Smallest possible mean particle size can be achieved by using low polymer concentration and high dispersion energy (enough sonication time) along with small aqueous/organic volume ratio.
authors.affiliation: Doctoral School of Molecular and Nanotechnologies, Faculty of Information Technology, University of Pannonia , Egyetem u.10, H-8200 Veszprém , Hungary .
authors.forename: Quazi T H
authors.initials: QT
authors.lastname: Shubhra
authors.forename: Andrea F
authors.initials: AF
authors.lastname: Kardos
authors.forename: Tivadar
authors.initials: T
authors.lastname: Feczkó
authors.forename: Hana
authors.initials: H
authors.lastname: Mackova
authors.forename: Daniel
authors.initials: D
authors.lastname: Horák
authors.forename: Judit
authors.initials: J
authors.lastname: Tóth
authors.forename: György
authors.initials: G
authors.lastname: Dósa
authors.forename: János
authors.initials: J
authors.lastname: Gyenis
mesh_terms: D005290:Ferric Compounds / Q000737:chemistry*; D006801:Humans; D019344:Lactic Acid / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D012709:Serum Albumin / Q000737:chemistry*
chemical_list: D005290:Ferric Compounds; D012709:Serum Albumin; C000499:ferric oxide; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid