title: Lysozyme detection on aptamer functionalized graphene-coated SPR interfaces.
abstract: The paper reports on a surface plasmon resonance (SPR)-based approach for the sensitive and selective detection of lysozyme. The SPR sensor consists of a 50 nm gold film coated with a thin film of reduced graphene oxide (rGO) functionalized with anti-lysozyme DNA aptamer. The SPR chip coating with rGO matrix was achieved through electrophoretic deposition of graphene oxide (GO) at 150 V. Electrophoretic deposition resulted in partial reduction of GO to rGO with a thickness depending on the deposition time. For very short time pulses of 20 s, the resulting rGO film had a thickness of several nanometers and was appropriate for SPR sensing. The utility of the graphene-based SPR sensor for the selective and sensitive detection of proteins was demonstrated using lysozyme as model protein. Functionalization of rGO matrix with anti-lysozyme DNA aptamer through π-stacking interactions allowed selective SPR detection of lysozyme. The graphene-based SPR biosensor provides a means for the label-free, concentration-dependent and selective detection of lysozymes with a detection limit of 0.5 nM.
authors.affiliation: Institut de Recherche Interdisciplinaire (IRI), CNRS USR 3078, Université Lille1, Parc de la Haute Borne, 50 avenue de Halley, BP 70478, 59658 Villeneuve d'Ascq, France.
authors.forename: Palaniappan
authors.initials: P
authors.lastname: Subramanian
authors.forename: Adam
authors.initials: A
authors.lastname: Lesniewski
authors.forename: Izabela
authors.initials: I
authors.lastname: Kaminska
authors.forename: Alexis
authors.initials: A
authors.lastname: Vlandas
authors.forename: Alina
authors.initials: A
authors.lastname: Vasilescu
authors.forename: Joanna
authors.initials: J
authors.lastname: Niedziolka-Jonsson
authors.forename: Emmanuelle
authors.initials: E
authors.lastname: Pichonat
authors.forename: Henri
authors.initials: H
authors.lastname: Happy
authors.forename: Rabah
authors.initials: R
authors.lastname: Boukherroub
authors.forename: Sabine
authors.initials: S
authors.lastname: Szunerits
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry*; D006108:Graphite / Q000737:chemistry*; D057230:Limit of Detection; D009113:Muramidase / Q000032:analysis*; D010087:Oxides / Q000737:chemistry; D020349:Surface Plasmon Resonance / Q000295:instrumentation*; D013499:Surface Properties
chemical_list: D052157:Aptamers, Nucleotide; D010087:Oxides; D006108:Graphite; D009113:Muramidase
keywords: Aptamer; Electrophoretic deposition; Lysozyme; Reduced graphene oxide; Surface plasmon resonance