title: Polychromatic angiography for the assessment of VEGF-induced BRB dysfunction in the rabbit retina.
abstract: OBJECTIVE
  To determine the utility of polychromatic angiography (PCA) in the assessment of VEGF-induced blood retinal barrier (BRB) dysfunction in rabbits.
  
  
  METHODS
  Twenty-six eyes of 24 Dutch Belted rabbits were injected intravitreally with 1.25 μg (group A, n = 5), 10 μg (group C, n = 7), or 4 μg (group B, n = 6; group D, n = 4; and group E, n = 4) of VEGF on day 0. Groups D and E were also injected intravitreally with 1.25 μg and 12.5 μg bevacizumab, respectively, on day 2. On days 0, 2, 4, 7, 11, and 14, PCA was performed using a contrast agent mixture composed of fluorescein sodium, indocyanine green, PCM102, and PCM107 and imaged with a modified fundus camera. PCA scores were based on detected leaking fluorophores.
  
  
  RESULTS
  On day 7, there was a statistically significant difference between PCA scores of group A (0.6 ± 0.89) and both groups B (2.67 ± 1.37, P = 0.0154) and C (3.33 ± 0.52, P = 0.00085). There was also a statistically significant difference between groups B and E (PCA score 0.75 ± 0.96, P = 0.032) on day 7. On day 11, there was statistically significant difference between group C (1.80 ± 1.1) and both groups A (0, P = 0.021) and B (0.33 ± 0.52, P = 0.037).
  
  
  CONCLUSIONS
  A differential response to both increasing VEGF dose and administration of bevacizumab could be discerned using the PCA. PCA allowed stratification of VEGF-induced BRB dysfunction and inhibitory effects of bevacizumab therapy in the rabbit retina.
authors.affiliation: PCAsso Diagnostics LLC, North Brunswick, New Jersey 08902, USA.
authors.forename: Samir R
authors.initials: SR
authors.lastname: Tari
authors.forename: Maher
authors.initials: M
authors.lastname: Youssif
authors.forename: C Michael
authors.initials: CM
authors.lastname: Samson
authors.forename: Robert L
authors.initials: RL
authors.lastname: Harris
authors.forename: Cheng-Mao
authors.initials: CM
authors.lastname: Lin
authors.forename: Uday B
authors.initials: UB
authors.lastname: Kompella
authors.forename: David A
authors.initials: DA
authors.lastname: Antonetti
authors.forename: Gaetano R
authors.initials: GR
authors.lastname: Barile
mesh_terms: D000818:Animals; D001813:Blood-Retinal Barrier / Q000187:drug effects*; D004195:Disease Models, Animal; D005451:Fluorescein Angiography / Q000379:methods*; D005654:Fundus Oculi; D058449:Intravitreal Injections; D008297:Male; D011817:Rabbits; D012160:Retina / Q000187:drug effects* / Q000473:pathology; D012164:Retinal Diseases / Q000139:chemically induced / Q000175:diagnosis* / Q000378:metabolism; D042461:Vascular Endothelial Growth Factor A / Q000008:administration & dosage / Q000633:toxicity*
chemical_list: D042461:Vascular Endothelial Growth Factor A
keywords: blood retinal barrier; polychromatic angiography; retina; retinal imaging