title: In vitro and in vivo evaluations of nano-hydroxyapatite/polyamide 66/glass fibre (n-HA/PA66/GF) as a novel bioactive bone screw.
abstract: In this study, we prepared nano-hydroxyapatite/polyamide 66/glass fibre (n-HA/PA66/GF) bioactive bone screws. The microstructure, morphology and coating of the screws were characterised, and the adhesion, proliferation and viability of MC3T3-E1 cells on n-HA/PA66/GF scaffolds were determined using scanning electron microscope, CCK-8 assays and cellular immunofluorescence analysis. The results confirmed that n-HA/PA66/GF scaffolds were biocompatible and had no negative effect on MC3T3-E1 cells in vitro. To investigate the in vivo biocompatibility, internal fixation properties and osteogenesis of the bioactive screws, both n-HA/PA66/GF screws and metallic screws were used to repair intercondylar femur fractures in dogs. General photography, CT examination, micro-CT examination, histological staining and biomechanical assays were performed at 4, 8, 12 and 24 weeks after operation. The n-HA/PA66/GF screws exhibited good biocompatibility, high mechanical strength and extensive osteogenesis in the host bone. Moreover, 24 weeks after implantation, the maximum push-out load of the bioactive screws was greater than that of the metallic screws. As shown by their good cytocompatibility, excellent biomechanical strength and fast formation and ingrowth of new bone, n-HA/PA66/GF screws are thus suitable for orthopaedic clinical applications.
authors.affiliation: Department of Orthopaedics, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China.
authors.forename: Bao
authors.initials: B
authors.lastname: Su
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Peng
authors.forename: Dianming
authors.initials: D
authors.lastname: Jiang
authors.forename: Jun
authors.initials: J
authors.lastname: Wu
authors.forename: Bo
authors.initials: B
authors.lastname: Qiao
authors.forename: Weichao
authors.initials: W
authors.lastname: Li
authors.forename: Xiaotong
authors.initials: X
authors.lastname: Qi
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology*; D001696:Biomechanical Phenomena / Q000187:drug effects; D001863:Bone Screws; D016923:Cell Death / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004285:Dogs; D017886:Durapatite / Q000494:pharmacology*; D005269:Femur / Q000000981:diagnostic imaging / Q000187:drug effects / Q000473:pathology; D005898:Glass / Q000737:chemistry*; D006801:Humans; D021621:Imaging, Three-Dimensional; D007477:Ions / Q000097:blood; D051379:Mice; D053758:Nanoparticles / Q000648:ultrastructure; D009757:Nylons / Q000494:pharmacology*; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects / Q000648:ultrastructure; D013052:Spectrometry, X-Ray Emission; D013194:Staining and Labeling; D054457:Tissue Scaffolds; D055114:X-Ray Microtomography
chemical_list: D001672:Biocompatible Materials; D007477:Ions; D009757:Nylons; C009753:fiberglass; D017886:Durapatite