title: Dermal cell damage induced by topical application of non-steroidal anti-inflammatory drugs is suppressed by trehalose co-lyophilization in ex vivo analysis.
abstract: Topical administration of non-steroidal anti-inflammatory drugs (NSAIDs) is generally considered safer than oral administration, although the former can occasionally induce cutaneous irritation. We hypothesized that the cutaneous irritation by topical NSAIDs might be suppressed by trehalose, which has protective effects on biological membranes. Using the three-dimensional cultured human skin model, Living Skin Equivalent-high, we found that cutaneous damage due to NSAIDs was reduced by concomitant use of trehalose and that this effect of trehalose was reinforced by co-lyophilization of NSAIDs with trehalose. The anti-inflammatory effect of co-lyophilized NSAIDs with trehalose was comparable to that seen with NSAIDs alone in a rat model. Our results suggest that co-lyophilization of NSAIDs with trehalose might be a novel procedure that can help prevent NSAIDs-induced skin irritation.
authors.affiliation: Department of Bioengineering, Graduate School of Engineering, The University of Tokyo, 2-11-16 Yayoi, Bunkyo-ku, Tokyo 113-8656, Japan.
authors.forename: Yuko
authors.initials: Y
authors.lastname: Kayasuga-Kariya
authors.forename: Shintaroh
authors.initials: S
authors.lastname: Iwanaga
authors.forename: Ayano
authors.initials: A
authors.lastname: Fujisawa
authors.forename: Lee-Shuan
authors.initials: LS
authors.lastname: Lin
authors.forename: Shigeki
authors.initials: S
authors.lastname: Suzuki
authors.forename: Ung-Il
authors.initials: UI
authors.lastname: Chung
authors.forename: Nobuo
authors.initials: N
authors.lastname: Sasaki
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Shimohata
authors.forename: Manabu
authors.initials: M
authors.lastname: Mochizuki
mesh_terms: D000287:Administration, Topical; D000704:Analysis of Variance; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000008:administration & dosage / Q000009:adverse effects* / Q000494:pharmacology*; D005612:Freeze Drying; D006801:Humans; D051381:Rats; D012867:Skin / Q000166:cytology / Q000187:drug effects*; D013778:Tetrazolium Salts; D013844:Thiazoles; D014199:Trehalose / Q000494:pharmacology*
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D013778:Tetrazolium Salts; D013844:Thiazoles; D014199:Trehalose; C022616:thiazolyl blue