title: Expression of odontogenic genes in human bone marrow mesenchymal stem cells.
abstract: OBJECTIVE
  Tooth loss is a common problem and since current tooth replacement methods cannot counter balance with biological tooth structures, regenerating natural tooth structures has become an ideal goal. A challenging problem in tooth regeneration is to find a proper clinically feasible cell to seed.This study was designed to investigate the odontogenic potential of human bone marrow mesenchymal stem cells (HBMSCs) for seeding in tooth regeneration.
  
  
  METHODS
  In this experimental study, three pregnant Sprague Dawley (SD) rats were used at the eleventh embryonic day and rat fetuses were removed surgically using semilunar flap under general anesthesia. The primary mandible was cut using a stereomicroscope. The epithelial and mesenchymal components were separated and the dissected oral epithelium was cultured for 3 days. We used flow cytometry analysis to confirm presence of mesenchymal stem cells and not hematopoietic cells and to demonstrate the presence of oral epithelium. Bone marrow mesenchymal stem cells (BMSCs) and cultured oral epithelium were then co-cultured for 14 days. BMSCs cultured alone were used as controls. Expression of two odontogenic genes Pax9 and DMP1 was assessed using quantitative reverse transcription- polymerase chain reaction (RT-PCR).
  
  
  RESULTS
  Expression of two odontogenic genes, Pax9 and DMP1, were detected in BMSCs co-cultured with oral epithelium but not in the control group.
  
  
  CONCLUSIONS
  Expression of Pax9 and DMP1 by human BMSCs in the proximity of odontogenic epithelium indicates odontogenic potential of these cells.
authors.affiliation: 1. Department of Oral and Maxillofacial Pathology, School of Dentistry, Shahid Beheshti Medical Science University, Tehran, Iran.
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Mashhadi Abbas
authors.forename: Hamed
authors.initials: H
authors.lastname: Sichani Fallahi
authors.forename: Ahad
authors.initials: A
authors.lastname: Khoshzaban
authors.forename: Nazanin
authors.initials: N
authors.lastname: Mahdavi
authors.forename: Seyedeh Sara
authors.initials: SS
authors.lastname: Bagheri
keywords: Bone Marrow Stem Cells; DMP1; Odontogenesis; Pax9