title: Development of a monoclonal antibody-based sandwich ELISA for peanut allergen Ara h 1 in food.
abstract: We have established a highly sensitive sandwich enzyme-linked immunosorbent assay (ELISA) based on two monoclonal antibodies (mAb) to measure the content of the major peanut allergen Ara h 1 in foods. Two mAbs were selected out of 12 murine hybridoma cells secreting Ara h 1-specific antibody. Using mAb 6 as the capture antibody and HRP-labelled mAb 4 as the detection antibody, the limit of detection (LOD) the assay was 0.34 ng/mL. Cross-reaction analysis showed that this method was strongly specific and had no cross-reactions with Ara h 2, pea protein or soy protein. Sample analysis showed that this ELISA was a useful tool to monitor peanut allergens in food products by measuring Ara h 1 content.
authors.affiliation: State Key Laboratory of Food Science & Technology, School of Food Science & Technology, Jiangnan University, Wuxi 214122, China. pengjuan2016@163.com
authors.forename: Juan
authors.initials: J
authors.lastname: Peng
authors.forename: Shanshan
authors.initials: S
authors.lastname: Song
authors.forename: Liguang
authors.initials: L
authors.lastname: Xu
authors.forename: Wei
authors.initials: W
authors.lastname: Ma
authors.forename: Liqiang
authors.initials: L
authors.lastname: Liu
authors.forename: Hua
authors.initials: H
authors.lastname: Kuang
authors.forename: Chuanlai
authors.initials: C
authors.lastname: Xu
mesh_terms: D000485:Allergens / Q000276:immunology*; D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis* / Q000276:immunology; D052179:Antigens, Plant / Q000276:immunology*; D019662:Cucurbitaceae; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005504:Food Analysis; D006023:Glycoproteins / Q000276:immunology*; D006825:Hybridomas; D008565:Membrane Proteins; D051379:Mice; D008892:Milk / Q000737:chemistry; D010940:Plant Proteins / Q000276:immunology*; D012639:Seeds / Q000737:chemistry; D013024:Soybean Oil / Q000032:analysis
chemical_list: D000485:Allergens; D000911:Antibodies, Monoclonal; D052179:Antigens, Plant; C500889:Ara h 1 protein, Arachis hypogaea; D006023:Glycoproteins; D008565:Membrane Proteins; D010940:Plant Proteins; D013024:Soybean Oil