title: A novel method for culturing human glottic cells.
abstract: OBJECTIVE
  The objective of this basic investigation was to describe a new method of preparing primary monolayer cultures of human glottic cells. To our knowledge, this is the first report of the culturing of healthy human glottic cells. This technique may be of use for other applications in the challenging field of laryngeal diseases.
  
  
  METHODS
  Individual prospective cohort study.
  
  
  METHODS
  Tissue samples were collected from 15 patients who underwent laryngeal surgery due to chronic laryngitis or larynx carcinoma. An inverted phase microscope was used to study the cultured cells, and immunocytochemistry using a mouse anti-human cytokeratin 19 monoclonal antibody was performed to identify epithelial cells. The relationship between the culture results and several patient variables was evaluated.
  
  
  RESULTS
  Cultures were positive in 40% of samples. The total and supracricoid laryngectomy groups had the highest rate of culture positivity (P < .02).
  
  
  CONCLUSIONS
  The current study provides methodological details that will allow other research groups to replicate this model of glottic cell culture.
authors.affiliation: ENT Department, Valencia University General Hospital, Valencia Medical School, Valencia, Spain.
authors.forename: Mireya
authors.initials: M
authors.lastname: Bonet
authors.forename: Jorge
authors.initials: J
authors.lastname: Basterra
authors.forename: Ana
authors.initials: A
authors.lastname: Pérez
authors.forename: Enrique
authors.initials: E
authors.lastname: Zapater
mesh_terms: D000328:Adult; D000368:Aged; D018929:Cell Culture Techniques / Q000379:methods*; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000473:pathology*; D005260:Female; D005500:Follow-Up Studies; D005931:Glottis / Q000473:pathology*; D006801:Humans; D007818:Laryngeal Diseases / Q000473:pathology* / Q000601:surgery; D007825:Laryngectomy; D008297:Male; D008858:Microscopy, Phase-Contrast; D008875:Middle Aged; D011446:Prospective Studies; D015203:Reproducibility of Results
keywords: Larynx; cytokeratin 19; glottis; monolayer culture; vocal fold