title: [Dissemination of blaIMP-8 among Enterobacteriaceae isolates from a Buenos Aires Hospital].
abstract: From August 2008 to December 2011, six metallo-β-lactamase-producing isolates, four Enterobacter cloacae, one Klebsiella oxytoca and one Citrobacter freundii, were detected at Hospital Interzonal General de Agudos "Evita" in Lanús. All six isolates showed multiresistant profiles and the presence of the blaIMP-8 gene. Five isolates also expressed PER-2 extended spectrum β-lactamase. The blaIMP-8 gene was found as the first cassette in a class 1 integron. However, the 3´ conserved sequence could not be detected in three isolates. In all cases, blaIMP-8 was transferred by conjugation to azide-resistant Escherichia coli J53. PFGE analysis revealed that the four E. cloacae isolates were not genetically related. These are the first metallo-β-lactamases detected in this institution and our results suggest a possible intra- and inter-species horizontal dissemination of blaIMP-8.
authors.affiliation: Laboratorio de Bacteriología, Hospital Interzonal General de Agudos, Lanús, Provincia de Buenos Aires, Argentina. anatogneri66@hotmail.com
authors.forename: Ana M
authors.initials: AM
authors.lastname: Togneri
authors.forename: Sonia A
authors.initials: SA
authors.lastname: Gómez
authors.forename: Laura B
authors.initials: LB
authors.lastname: Podestá
authors.forename: Marcela P
authors.initials: MP
authors.lastname: Pérez
authors.forename: Diego F
authors.initials: DF
authors.lastname: Faccone
authors.forename: Lidia E
authors.initials: LE
authors.lastname: Ríos
authors.forename: Marcelo A
authors.initials: MA
authors.lastname: Gañetea
authors.forename: María S
authors.initials: MS
authors.lastname: Anchordoqui
authors.forename: Fernando G
authors.initials: FG
authors.lastname: Pasterán
authors.forename: Alejandra C
authors.initials: AC
authors.lastname: Corso
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D001118:Argentina; D004755:Enterobacteriaceae / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification; D005260:Female; D006761:Hospitals; D006801:Humans; D008297:Male; D008875:Middle Aged; D001618:beta-Lactamases / Q000235:genetics
chemical_list: D001618:beta-Lactamases