title: Hepatoprotective effects of eburicoic acid and dehydroeburicoic acid from Antrodia camphorata in a mouse model of acute hepatic injury.
abstract: The hepatoprotective effects of eburicoic acid (TR1) and dehydroeburicoic acid (TR2) from Antrodia camphorata (AC) against carbon tetrachloride (CCl4)-induced liver damage were investigated in mice. TR1 and TR2 was administered intraperitoneally (i.p.) for 7 days prior to the administration of CCl4. Pretreatment with TR1 and TR2 prevented the elevation of aspartate aminotransferase (AST), alanine aminotransferase (ALT), and liver lipid peroxides in CCl4-treated mice. The activities of antioxidant enzymes [catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GPx)], nitric oxide (NO) production, and tumour necrosis factor-alpha (TNF-α) were decreased after the treatment with TR1 and TR2 in CCl4-treated mice. Western blotting revealed that TR1 and TR2 significantly decreased inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) expressions and increased the expression of cytochrome P4502E1 (CYP2E1) in CCl4-treated mice. Therefore, we speculate that TR1 and TR2 protect the liver from CCl4-induced hepatic damage via antioxidant and anti-inflammatory mechanisms.
authors.affiliation: Department of Chinese Pharmaceutical Sciences and Chinese Medicine Resources, College of Pharmacy, China Medical University, Taichung 404, Taiwan.
authors.forename: Guan-Jhong
authors.initials: GJ
authors.lastname: Huang
authors.forename: Jeng-Shyan
authors.initials: JS
authors.lastname: Deng
authors.forename: Shyh-Shyun
authors.initials: SS
authors.lastname: Huang
authors.forename: Chao-Ying
authors.initials: CY
authors.lastname: Lee
authors.forename: Wen-Chi
authors.initials: WC
authors.lastname: Hou
authors.forename: Sheng-Yang
authors.initials: SY
authors.lastname: Wang
authors.forename: Ping-Jyun
authors.initials: PJ
authors.lastname: Sung
authors.forename: Yueh-Hsiung
authors.initials: YH
authors.lastname: Kuo
mesh_terms: D000208:Acute Disease / Q000628:therapy; D000410:Alanine Transaminase / Q000235:genetics / Q000378:metabolism; D000818:Animals; D055491:Antrodia / Q000737:chemistry*; D001219:Aspartate Aminotransferases / Q000235:genetics / Q000378:metabolism; D002251:Carbon Tetrachloride / Q000009:adverse effects; D056486:Chemical and Drug Induced Liver Injury / Q000188:drug therapy / Q000201:enzymology / Q000209:etiology / Q000517:prevention & control*; D004195:Disease Models, Animal; D005979:Glutathione Peroxidase / Q000235:genetics / Q000378:metabolism; D006801:Humans; D007810:Lanosterol / Q000008:administration & dosage / Q000031:analogs & derivatives*; D008099:Liver / Q000187:drug effects / Q000201:enzymology; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D009569:Nitric Oxide / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism
chemical_list: C547186:dehydroeburicoic acid; D007810:Lanosterol; D009569:Nitric Oxide; C004867:eburicoic acid; D002251:Carbon Tetrachloride; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase
keywords: Anti-inflammation; Antrodia camphorata; Dehydroeburicoic acid; Eburicoic acid; NO; TNF-α