title: Synthetic ePTFE grafts coated with an anti-CD133 antibody-functionalized heparin/collagen multilayer with rapid in vivo endothelialization properties.
abstract: An anti-CD133 antibody multilayer functionalized by heparin/collagen on an expanded polytetrafluoroethylene (ePTFE) graft was developed to accelerate early endothelialization. The surface modification of ePTFE grafts demonstrated that the multilayer is stable in static incubation and shaking conditions and that the anti-CD133 antibodies were successfully cross-linked onto the surface. Blood compatibility tests revealed that the coimmobilized heparin/collagen films in the presence or absence of anti-CD133 antibodies prolonged the blood coagulation time and that there was less platelet activation and aggregation, whereas the hemolysis rate was comparable with the bare ePTFE grafts. Cellular proliferation was not inhibited, as the heparin/collagen synthetic vascular grafts coated with CD133 antibody showed little cytotoxicity. The endothelial cells adhered well to the modified ePTFE grafts during a cell adhesion assay. A porcine carotid artery transplantation model was used to evaluate the modified ePTFE grafts in vivo. The results of histopathological staining and scanning electron microscopy indicated that the anti-CD133 antibody was able to accelerate the attachment of vascular endothelial cells onto the ePTFE grafts, resulting in early rapid endothelialization. The success of the anti-CD133 antibody-functionalized heparin/collagen multilayer will provide an effective selection system for the surface modification of synthetic vascular grafts and improve their use in clinical applications.
authors.affiliation: Shanghai Institute of Cardiovascular Disease, Zhongshan Hospital, Fudan University, Shanghai 200032, China.
authors.forename: Shuyang
authors.initials: S
authors.lastname: Lu
authors.forename: Peng
authors.initials: P
authors.lastname: Zhang
authors.forename: Xiaoning
authors.initials: X
authors.lastname: Sun
authors.forename: Feirong
authors.initials: F
authors.lastname: Gong
authors.forename: Shouguo
authors.initials: S
authors.lastname: Yang
authors.forename: Li
authors.initials: L
authors.lastname: Shen
authors.forename: Zheyong
authors.initials: Z
authors.lastname: Huang
authors.forename: Chunsheng
authors.initials: C
authors.lastname: Wang
mesh_terms: D000071916:AC133 Antigen; D000818:Animals; D000906:Antibodies / Q000737:chemistry*; D015703:Antigens, CD / Q000737:chemistry*; D001780:Blood Coagulation Tests; D001807:Blood Vessel Prosthesis; D002339:Carotid Arteries / Q000378:metabolism; D020099:Coated Materials, Biocompatible / Q000737:chemistry; D003094:Collagen / Q000737:chemistry*; D042783:Endothelial Cells / Q000166:cytology; D006023:Glycoproteins / Q000737:chemistry*; D006461:Hemolysis; D006493:Heparin / Q000737:chemistry*; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D008297:Male; D008855:Microscopy, Electron, Scanning / Q000379:methods; D010455:Peptides / Q000737:chemistry*; D010973:Platelet Adhesiveness; D011138:Polytetrafluoroethylene / Q000737:chemistry*; D013552:Swine
chemical_list: D000071916:AC133 Antigen; D000906:Antibodies; D015703:Antigens, CD; D020099:Coated Materials, Biocompatible; D006023:Glycoproteins; C000606023:PROM1 protein, human; D010455:Peptides; D011138:Polytetrafluoroethylene; D006493:Heparin; D003094:Collagen