title: [Effects of chain length of polyacrylic acid (PAA) on proteins adsorption of polystyrene-polyacrylic acid (PS-PAA) spherical polyelectrolyte brushes].
abstract: We studied the interaction between proteins and polystyrene-polyacrylic acid (PS-PAA) spherical polyelectrolyte brushes with different polyacrylic acid (PAA) chain lengths, including the physical adsorption and chemical adsorption in PBS buffer. Results showed that the amount of bovine serum albumin (BSA) physically adsorbed on PS-PAA spherical polyelectrolyte brushes decreased to a minimum of 33 microg/mg whereas the amount of streptavidin (SA) chemically adsorbed increased with the increase of chain length and carboxyl quantity. The biotin binding capacity of streptavidin chemically adsorbed on PS-PAA spherical polyelectrolyte brushes was roughly evaluated via enzyme competitive inhibition.
authors.affiliation: Nano Biomedical Research Center, School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai 200030, China.
authors.forename: Yanping
authors.initials: Y
authors.lastname: Liu
authors.forename: Yicun
authors.initials: Y
authors.lastname: Wen
authors.forename: Hong
authors.initials: H
authors.lastname: Xu
authors.forename: Xuhong
authors.initials: X
authors.lastname: Guo
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry*; D000327:Adsorption; D015374:Biosensing Techniques; D055664:Electrochemical Techniques / Q000379:methods*; D004573:Electrolytes / Q000737:chemistry; D011073:Polyamines; D000071228:Polyelectrolytes; D011108:Polymers / Q000737:chemistry; D011137:Polystyrenes / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry; D013499:Surface Properties
chemical_list: D000180:Acrylic Resins; D004573:Electrolytes; D011073:Polyamines; D000071228:Polyelectrolytes; D011108:Polymers; D011137:Polystyrenes; D011506:Proteins; D012710:Serum Albumin, Bovine; C002189:polyelectrolyte 60; C006903:carbopol 940