title: Interleukin-10 prevents epithelial cell apoptosis by regulating IFNγ and TNFα expression in rhesus macaque colon explants.
abstract: Interleukin-10 (IL-10) is an important immunomodulatory cytokine that plays an obligate role in regulating inflammatory responses. Here we demonstrated the role of IL-10 in regulating crypts length and breadth as well as maintaining the survival of epithelial cells using rhesus colon explant cultures. Anti-IL-10 antibody treatment of colon explant cultures induced increased production of inflammatory cytokines/molecules like IFNγ, TNFα, CD107a and perforin as well as increased epithelial cell apoptosis compared to media controls tested. Our results suggest that IL-10 plays a crucial role in maintaining mucosal homeostasis by regulating mucosal IFNγ and TNFα cytokine production.
authors.affiliation: Division of Comparative Pathology, Tulane National Primate Research Center, Covington, LA, USA.
authors.forename: Diganta
authors.initials: D
authors.lastname: Pan
authors.forename: Arpita
authors.initials: A
authors.lastname: Das
authors.forename: Wendy
authors.initials: W
authors.lastname: Lala
authors.forename: Carys S
authors.initials: CS
authors.lastname: Kenway-Lynch
authors.forename: David X
authors.initials: DX
authors.lastname: Liu
authors.forename: Ronald S
authors.initials: RS
authors.lastname: Veazey
authors.forename: Bapi
authors.initials: B
authors.lastname: Pahar
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D017209:Apoptosis; D003106:Colon / Q000378:metabolism; D004847:Epithelial Cells / Q000378:metabolism*; D005260:Female; D007371:Interferon-gamma / Q000096:biosynthesis / Q000378:metabolism*; D016753:Interleukin-10 / Q000276:immunology / Q000378:metabolism*; D007413:Intestinal Mucosa / Q000166:cytology / Q000378:metabolism; D052118:Lysosomal-Associated Membrane Protein 1 / Q000096:biosynthesis / Q000378:metabolism; D008253:Macaca mulatta; D008297:Male; D009924:Organ Culture Techniques; D054353:Perforin / Q000096:biosynthesis / Q000378:metabolism; D016097:Simian Acquired Immunodeficiency Syndrome / Q000276:immunology; D015302:Simian Immunodeficiency Virus; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis / Q000378:metabolism*
chemical_list: D000911:Antibodies, Monoclonal; D052118:Lysosomal-Associated Membrane Protein 1; D014409:Tumor Necrosis Factor-alpha; D054353:Perforin; D016753:Interleukin-10; D007371:Interferon-gamma
keywords: AC-3; Apoptosis; Colon; Cytokine; EC; Epithelial cell; HIV; IL-10; JAK1; MAb; RM; SCID; SIV; T-helper 1; Th1; Tyk2; active caspase-3; epithelial cells; human immunodeficiency virus; interleukin-10; janus kinase 1; monoclonal antibody; rIL-10; recombinant IL-10; rhesus macaque; severe combined immunodeficiency; simian immunodeficiency virus; tyrosine kinase 2