title: A simple method for deriving functional MSCs and applied for osteogenesis in 3D scaffolds.
abstract: We describe a simple method for bone engineering using biodegradable scaffolds with mesenchymal stem cells derived from human induced-pluripotent stem cells (hiPS-MSCs). The hiPS-MSCs expressed mesenchymal markers (CD90, CD73, and CD105), possessed multipotency characterized by tri-lineages differentiation: osteogenic, adipogenic, and chondrogenic, and lost pluripotency - as seen with the loss of markers OCT3/4 and TRA-1-81 - and tumorigenicity. However, these iPS-MSCs are still positive for marker NANOG. We further explored the osteogenic potential of the hiPS-MSCs in synthetic polymer polycaprolactone (PCL) scaffolds or PCL scaffolds functionalized with natural polymer hyaluronan and ceramic TCP (PHT) both in vitro and in vivo. Our results showed that these iPS-MSCs are functionally compatible with the two 3D scaffolds tested and formed typically calcified structure in the scaffolds. Overall, our results suggest the iPS-MSCs derived by this simple method retain fully osteogenic function and provide a new solution towards personalized orthopedic therapy in the future.
authors.affiliation: Orthopaedic Research Laboratory, Department of Orthopaedics, Aarhus University Hospital, Aarhus, DK, Denmark.
authors.forename: Lijin
authors.initials: L
authors.lastname: Zou
authors.forename: Yonglun
authors.initials: Y
authors.lastname: Luo
authors.forename: Muwan
authors.initials: M
authors.lastname: Chen
authors.forename: Gang
authors.initials: G
authors.lastname: Wang
authors.forename: Ming
authors.initials: M
authors.lastname: Ding
authors.forename: Charlotte Christie
authors.initials: CC
authors.lastname: Petersen
authors.forename: Ran
authors.initials: R
authors.lastname: Kang
authors.forename: Frederik
authors.initials: F
authors.lastname: Dagnaes-Hansen
authors.forename: Yuanlin
authors.initials: Y
authors.lastname: Zeng
authors.forename: Nonghua
authors.initials: N
authors.lastname: Lv
authors.forename: Qing
authors.initials: Q
authors.lastname: Ma
authors.forename: Dang Q S
authors.initials: DQ
authors.lastname: Le
authors.forename: Flemming
authors.initials: F
authors.lastname: Besenbacher
authors.forename: Lars
authors.initials: L
authors.lastname: Bolund
authors.forename: Thomas G
authors.initials: TG
authors.lastname: Jensen
authors.forename: Jørgen
authors.initials: J
authors.lastname: Kjems
authors.forename: William T
authors.initials: WT
authors.lastname: Pu
authors.forename: Cody
authors.initials: C
authors.lastname: Bünger
mesh_terms: D000818:Animals; D002113:Calcification, Physiologic; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D019070:Cell Lineage; D005347:Fibroblasts / Q000166:cytology; D005786:Gene Expression Regulation; D006801:Humans; D016130:Immunophenotyping; D057026:Induced Pluripotent Stem Cells / Q000166:cytology / Q000378:metabolism; D059785:Karyotype; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D051379:Mice; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism; D010012:Osteogenesis / Q000502:physiology*; D010641:Phenotype; D012333:RNA, Messenger / Q000235:genetics; D013997:Time Factors; D054457:Tissue Scaffolds
chemical_list: D012333:RNA, Messenger