title: Microparticles prepared from sulfenamide-based polymers.
abstract: Polysulfenamides (PSN), with a SN linkage (RSNR2) along the polymer backbone, are a new class of biodegradable and biocompatible polymers. These polymers were unknown prior to 2012 when their synthesis and medicinally relevant properties were reported. The aim of this study was to develop microparticles as a controlled drug delivery system using polysulfenamide as the matrix material. The microparticles were prepared by a water-in-oil-in-water double-emulsion solvent-evaporation method. For producing drug-loaded particles, FITC-dextran was used as a model hydrophilic compound. At the optimal formulation conditions, the external morphology of the PSN microparticles was examined by scanning electron microscopy to show the formation of smooth-surfaced spherical particles with low polydispersity. The microparticles had a net negative surface charge (-23 mV) as analyzed by the zetasizer. The drug encapsulation efficiency of the particles and the drug loading were found to be dependent on the drug molecular weight, amount of FITC-dextran used in fabricating FITC-dextran-loaded microparticles, concentration of PSN and surfactant, and volume of the internal and external water phases. FITC-dextran was found to be distributed throughout the PSN microparticles and was released in an initial burst followed by more continuous release over time. Confocal laser scanning microscopy was used to qualitatively observe the cellular uptake of PSN microparticles and indicated localization of the particles in both the cytoplasm and the nucleus.
authors.affiliation: Department of Pharmaceutical Sciences & Experimental Therapeutics, College of Pharmacy, University of Iowa , Iowa City, IA , USA and.
authors.forename: Sheetal R
authors.initials: SR
authors.lastname: D'Mello
authors.forename: Jun
authors.initials: J
authors.lastname: Yoo
authors.forename: Ned B
authors.initials: NB
authors.lastname: Bowden
authors.forename: Aliasger K
authors.initials: AK
authors.lastname: Salem
mesh_terms: D002214:Capsules / Q000737:chemistry / Q000494:pharmacology; D003692:Delayed-Action Preparations / Q000737:chemistry / Q000494:pharmacology; D004655:Emulsions / Q000737:chemistry / Q000494:pharmacology; D057809:HEK293 Cells; D006801:Humans; D011108:Polymers / Q000737:chemistry* / Q000494:pharmacology; D013416:Sulfamerazine / Q000737:chemistry* / Q000494:pharmacology
chemical_list: D002214:Capsules; D003692:Delayed-Action Preparations; D004655:Emulsions; D011108:Polymers; C471392:sulfenamide; D013416:Sulfamerazine