title: Mitochondria-targeted peptides prevent on contrast-induced acute kidney injury in the rats with hypercholesterolemia.
abstract: OBJECTIVE
  The objective of this study is to evaluate the effect and mechanism of mitochondria-targeted peptides (MTP131 and SPI20) on contrast-induced acute kidney injury (CI-AKI) in rats with hypercholesterolemia.
  
  
  METHODS
  Forty SD rats were randomly divided into normal diet group (NN, n = 8) and high cholesterol supplemented dietary group (HN, n = 32). At the end of 8 weeks, the group HN was divided into four subgroups. All Rats were given injection of either diatrizoate (10 mL/kg) or equal volume of normal saline, the rats pretreated with MTP131 or SPI20 were given injection with MTP131 or SPI 20 (3 mg/kg) by peritoneal cavity for 3 times. Blood, urine and renal tissue samples were prepared to determine biochemical parameters. The renal pathological changes were evaluated by hematoxylin and eosin staining and scored semiquantitatively, The protein expression of renal NOX4 was also measured by Western blotting.
  
  
  RESULTS
  In diatrizoate-injected rats, Serum creatinine (Scr), fractional excretion of sodium (FeNa%), fractional excretion of potassium (FeK%), pathological scores, renal malondialdehyde (MDA) content, the NADPH oxidase activity and the expression of NOX4 in kidney tissue were significantly increased (p < 0.01). In the groups pretreated with MTP131 or SPI20, the levels of Scr, FeNa%, FeK%, MDA content and NADPH oxidase activity in renal tissue decreased (p < 0.01), the levels of renal super oxygen dehydrogenises and ATPase activity increased (p < 0.01). The renal injuries induced by contrast media (CM) were alleviated.
  
  
  CONCLUSIONS
  MTP131 and SPI20 might protect acute kidney injury induced by CM in rats with hypercholesterolemia.
authors.affiliation: Department of Nephrology, Second Xiangya Hospital of Central South University, Nephrology Institute of Central South University, Centre of Kidney Disease and Dialysis in Hunan Province, Changsha, China. Duansb528@hotmail.com
authors.forename: Shao-Bin
authors.initials: SB
authors.lastname: Duan
authors.forename: Shi-Kun
authors.initials: SK
authors.lastname: Yang
authors.forename: Qiao-Yan
authors.initials: QY
authors.lastname: Zhou
authors.forename: Peng
authors.initials: P
authors.lastname: Pan
authors.forename: Hui
authors.initials: H
authors.lastname: Zhang
authors.forename: Fang
authors.initials: F
authors.lastname: Liu
authors.forename: Xiang-Qing
authors.initials: XQ
authors.lastname: Xu
mesh_terms: D058186:Acute Kidney Injury / Q000139:chemically induced* / Q000517:prevention & control*; D000818:Animals; D003287:Contrast Media / Q000009:adverse effects*; D003973:Diatrizoate / Q000009:adverse effects; D004195:Disease Models, Animal; D006937:Hypercholesterolemia / Q000150:complications*; D008297:Male; D009842:Oligopeptides / Q000627:therapeutic use*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D003287:Contrast Media; D009842:Oligopeptides; C506540:arginyl-2,'6'-dimethyltyrosyl-lysyl-phenylalaninamide; C520830:phenylalanyl-arginyl-phenylalanyllysinamide; D003973:Diatrizoate