title: Identification of clinical Pasteurella isolates by MALDI-TOF -- a comparison with VITEK 2 and conventional microbiological methods.
abstract: The aim of this study was to compare the performance of four methods that are widely used in the clinical microbiology laboratory for identification of Pasteurella species. The 4 methods evaluated were VITEK2, VITEK MS (BioMerieux), and Bruker Biotyper MS (Bruker) as well as traditional biochemical tests. Sequencing of the sodA gene was used as the reference method. Sixty-five isolates of Pasteurella spp. from 65 patients were analyzed. One Pasteurella multocida isolate from American Type Culture Collection (Manassas, VA, USA) was used as a reference. Traditional biochemical tests accurately identified 62/66 (94%) isolates. Both Bruker and Vitek matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) identified 59/66 (89%) strains, but VITEK2 could only identify 32/66 (48.5%) isolates correctly. The mean time to identification using biochemical tests was 20 hours; VITEK2 took 6 hours and MALDI-TOF approximately 10 minutes. In conclusion, MALDI-TOF is a quick method, which accurately identified most isolates of Pasteurella to the species level. Thus, MALDI-TOF constitutes a valuable diagnostic tool in the clinical laboratory.
authors.affiliation: Department of Laboratory Medicine, Division of Clinical Microbiology, Karolinska Institutet and Karolinska University Hospital, Huddinge, Sweden; Department of Medicine, Centre for Infectious Medicine (CIM), Karolinska Institutet and Karolinska University Hospital, Huddinge, Sweden. Electronic address: salah.zangenah@ki.se.
authors.forename: Salah
authors.initials: S
authors.lastname: Zangenah
authors.forename: Gülay
authors.initials: G
authors.lastname: Güleryüz
authors.forename: Stina
authors.initials: S
authors.lastname: Boräng
authors.forename: Måns
authors.initials: M
authors.lastname: Ullberg
authors.forename: Peter
authors.initials: P
authors.lastname: Bergman
authors.forename: Volkan
authors.initials: V
authors.lastname: Ozenci
mesh_terms: D001431:Bacteriological Techniques / Q000379:methods*; D005260:Female; D006801:Humans; D008297:Male; D010325:Pasteurella / Q000737:chemistry* / Q000145:classification* / Q000302:isolation & purification; D010326:Pasteurella Infections / Q000175:diagnosis / Q000382:microbiology*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
keywords: MALDI-TOF; Pasteurella spp; Vitek2; animal bite infections