title: Gram-scale production of a basidiomycetous laccase in Aspergillus niger.
abstract: We report on the expression in Aspergillus niger of a laccase gene we used to produce variants in Saccharomyces cerevisiae. Grams of recombinant enzyme can be easily obtained. This highlights the potential of combining this generic laccase sequence to the yeast and fungal expression systems for large-scale productions of variants.
authors.affiliation: Aix Marseille Université, CNRS, iSm2 UMR 7313, 13397 Marseille, France. Electronic address: y.mekmouche@univ-amu.fr.
authors.forename: Yasmina
authors.initials: Y
authors.lastname: Mekmouche
authors.affiliation: Aix Marseille Université, CNRS, iSm2 UMR 7313, 13397 Marseille, France; Aix-Marseille Université, INRA, UMR 1163, Biotechnologie des Champignons Filamenteux, Faculté des Sciences de Luminy-Polytech, Case 925, 13009 Marseille, France.
authors.forename: Simeng
authors.initials: S
authors.lastname: Zhou
authors.affiliation: Aix Marseille Université, CNRS, iSm2 UMR 7313, 13397 Marseille, France.
authors.forename: Angela M
authors.initials: AM
authors.lastname: Cusano
authors.affiliation: Aix-Marseille Université, INRA, UMR 1163, Biotechnologie des Champignons Filamenteux, Faculté des Sciences de Luminy-Polytech, Case 925, 13009 Marseille, France.
authors.forename: Eric
authors.initials: E
authors.lastname: Record
authors.affiliation: Aix-Marseille Université, INRA, UMR 1163, Biotechnologie des Champignons Filamenteux, Faculté des Sciences de Luminy-Polytech, Case 925, 13009 Marseille, France.
authors.forename: Anne
authors.initials: A
authors.lastname: Lomascolo
authors.affiliation: Aix Marseille Université, CNRS, iSm2 UMR 7313, 13397 Marseille, France.
authors.forename: Viviane
authors.initials: V
authors.lastname: Robert
authors.affiliation: Aix Marseille Université, CNRS, iSm2 UMR 7313, 13397 Marseille, France.
authors.forename: A Jalila
authors.initials: AJ
authors.lastname: Simaan
authors.affiliation: Aix Marseille Université, CNRS, iSm2 UMR 7313, 13397 Marseille, France.
authors.forename: Pierre
authors.initials: P
authors.lastname: Rousselot-Pailley
authors.affiliation: Aix Marseille Université, CNRS, iSm2 UMR 7313, 13397 Marseille, France.
authors.forename: Sana
authors.initials: S
authors.lastname: Ullah
authors.affiliation: Aix-Marseille Université, CNRS, IMBE, UMR 7263, Laboratoire Chimie Physique et Prévention des Risques et Nuisances Technologiques, FR3098 ECCOREV, Faculté de Pharmacie, F-13005 Marseille, France.
authors.forename: Florence
authors.initials: F
authors.lastname: Chaspoul
authors.affiliation: Aix Marseille Université, CNRS, iSm2 UMR 7313, 13397 Marseille, France.
authors.forename: Thierry
authors.initials: T
authors.lastname: Tron
mesh_terms: D001234:Aspergillus niger / Q000201:enzymology* / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D006863:Hydrogen-Ion Concentration; D042845:Laccase / Q000096:biosynthesis* / Q000235:genetics; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000378:metabolism
chemical_list: D011994:Recombinant Proteins; D042845:Laccase
keywords: Aspergillus niger; Bioreactor; Glycosylation; Multicopper enzyme; Saccharomyces cerevisiae