title: Enhanced adhesion of preosteoblasts inside 3D PCL scaffolds by polydopamine coating and mineralization.
abstract: In tissue engineering, fabrication of 3D scaffolds with well-defined, inter-connected pores followed by culture of mammalian cells is a typical approach. In practice, however, hydrophobicity of scaffold surfaces is not suitable for cells to be adhered because of poor wettability. Especially, infiltration followed by adhesion of cells inside hydrophobic scaffolds remains as a challenge. Thus, hydrophilic conversions of the surfaces regardless of surface location are critical for success. Herein, a method to enhance infiltration and adhesion of preosteoblasts inside hydrophobic poly(ϵ-caprolactone) (PCL) scaffolds by a bio-inspired, hydroxyapatite formation is demonstrated. The approach can be a general method for controlling hydrophilicity of inner surfaces of scaffolds.
authors.affiliation: Department of Chemistry, KAIST, Daejeon, 305-701, South Korea.
authors.forename: Sunae
authors.initials: S
authors.lastname: Jo
authors.forename: Sung Min
authors.initials: SM
authors.lastname: Kang
authors.forename: Su A
authors.initials: SA
authors.lastname: Park
authors.forename: Wan Doo
authors.initials: WD
authors.lastname: Kim
authors.forename: Juhyoun
authors.initials: J
authors.lastname: Kwak
authors.forename: Haeshin
authors.initials: H
authors.lastname: Lee
mesh_terms: D002113:Calcification, Physiologic; D002448:Cell Adhesion / Q000187:drug effects* / Q000502:physiology; D002478:Cells, Cultured; D057927:Hydrophobic and Hydrophilic Interactions; D007211:Indoles / Q000737:chemistry / Q000494:pharmacology; D010006:Osteoblasts / Q000737:chemistry / Q000187:drug effects*; D011091:Polyesters / Q000737:chemistry* / Q000494:pharmacology; D011108:Polymers / Q000737:chemistry / Q000494:pharmacology; D013499:Surface Properties; D054457:Tissue Scaffolds; D017155:Wettability
chemical_list: D007211:Indoles; D011091:Polyesters; D011108:Polymers; C568283:polydopamine; C016240:polycaprolactone
keywords: 3D-scaffold; cell infiltration; hydroxyapatite; polycaprolactone (PCL); tissue engineering