title: Oocyte morphology on day 0 correlates with aneuploidy as detected by polar body biopsy and FISH.
abstract: OBJECTIVE
  For better selection of oocytes and embryos, preimplantation genetic screening (PGS) was introduced. As from the beginning of IVF, morphology was used as selection criteria; we investigated the combination of both. If there was a correlation between phenotype and genotype, invasive PGS might be replaced.
  
  
  METHODS
  Therefore, 104 cycles with PGS were done by biopsy of the first polar body and FISH with five chromosomes. Morphology of the oocyte was recorded digitally and noted for 12 categories in 4-13 values; evaluation of the chromosomes was noted for five chromosomes in five values. Morphology and genetics were correlated to each other.
  
  
  RESULTS
  Correlations between morphology and genetics for day 0 were found: oocytes with an irregular or dark zona are less probable to have a normal chromosome 13 (80 vs. 53 %, p = 0.001). A medium amount of detritus in the perivitelline space makes it more probable to have a normal chromosome 18 (94 vs. 78 %, p = 0.001). A halo in the cytoplasm makes it less probable to be euploid for chromosome 22 (56 vs. 75 %, p = 0.018). For day 1, pattern "1, 2, 3 and fine" in the pronuclei makes it more probable to be euploid for chromosome 22 (78 vs. 63 %, p = 0.002).
  
  
  CONCLUSIONS
  There are correlations between the oocyte genome and its morphology also on day 0. These correlations are not sufficient to replace PGS.
authors.affiliation: Women's Hospital, Christian-Albrechts-University, Kiel, Germany, schmutzler@email.uni-kiel.de.
authors.forename: Andreas G
authors.initials: AG
authors.lastname: Schmutzler
authors.forename: Bengi
authors.initials: B
authors.lastname: Acar-Perk
authors.forename: Jörg
authors.initials: J
authors.lastname: Weimer
authors.forename: Ali
authors.initials: A
authors.lastname: Salmassi
authors.forename: Knud
authors.initials: K
authors.lastname: Sievers
authors.forename: Monica
authors.initials: M
authors.lastname: Tobler
authors.forename: Liselotte
authors.initials: L
authors.lastname: Mettler
authors.forename: Walter
authors.initials: W
authors.lastname: Jonat
authors.forename: Norbert
authors.initials: N
authors.lastname: Arnold
mesh_terms: D000328:Adult; D000782:Aneuploidy; D001706:Biopsy / Q000379:methods*; D002882:Chromosomes, Human, Pair 13; D002885:Chromosomes, Human, Pair 16; D002887:Chromosomes, Human, Pair 18; D002891:Chromosomes, Human, Pair 21; D002892:Chromosomes, Human, Pair 22; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D009865:Oocytes; D059705:Polar Bodies; D011247:Pregnancy; D019836:Preimplantation Diagnosis