title: p53 death signal is mainly mediated by Nuc1(EndoG) in the yeast Saccharomyces cerevisiae.
abstract: The tumor suppressor p53 plays a central role in the regulation of cellular growth and apoptosis. In the yeast Saccharomyces cerevisiae, the overexpression of the human p53 leads to growth inhibition and apoptotic cell death on minimal medium. In the present work, we show that p53-expressing cells are more susceptible to cell death after an apoptotic stimulus such as H2O2. The analysis of mutants involved in yeast apoptosis-like death suggests that the observed cell death is Yca1 independent and mainly mediated through Nuc1p.
authors.affiliation: Pasteur Institute-Cenci Bolognetti Foundation, Department of Biology and Biotechnology "Charles Darwin", University "Sapienza" of Rome, Rome, Italy.
authors.forename: Vanessa
authors.initials: V
authors.lastname: Palermo
authors.forename: Eleonora
authors.initials: E
authors.lastname: Mangiapelo
authors.forename: Cristina
authors.initials: C
authors.lastname: Piloto
authors.forename: Luisa
authors.initials: L
authors.lastname: Pieri
authors.forename: Michela
authors.initials: M
authors.lastname: Muscolini
authors.forename: Loretta
authors.initials: L
authors.lastname: Tuosto
authors.forename: Cristina
authors.initials: C
authors.lastname: Mazzoni
mesh_terms: D017209:Apoptosis; D020169:Caspases / Q000378:metabolism; D003470:Culture Media / Q000737:chemistry; D004720:Endonucleases / Q000378:metabolism*; D005092:Exonucleases / Q000378:metabolism*; D015870:Gene Expression; D006801:Humans; D006861:Hydrogen Peroxide / Q000633:toxicity; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism*; D015398:Signal Transduction; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism*
chemical_list: D003470:Culture Media; D029701:Saccharomyces cerevisiae Proteins; D016159:Tumor Suppressor Protein p53; D006861:Hydrogen Peroxide; D004720:Endonucleases; D005092:Exonucleases; C587067:Nuc1 protein, S cerevisiae; D020169:Caspases; C456272:MCA1 protein, S cerevisiae
keywords: Nuc1; apoptosis; chronological life span; p53; yeast