title: Scale-up and kinetic modeling for bioethanol production.
abstract: Bioethanol was produced from acidic hydrolysate of rice hulls using recombinant Escherichia coli KO11. Two different issues (scale-up and kinetic modeling) were evaluated simultaneously and concomitantly for bioethanol production. During the step-wise scale-up process from 100 mL shaken flask to 10 L stirred-tank bioreactor, the constant Reynolds number and the constant impeller tip speed were evaluated as scale-up methodologies under laboratory conditions. It was determined that the volumetric bioethanol productivity was 88% higher in 10 L bioreactor in comparison to the value of 0.21 g L(-1) h(-1) in shaken flask. The modified Monod and Luedeking-Piret models provided an accurate approach for the modeling of the experimental data. Ethanol concentration reached the maximum level of 29.03 g/L, which was 5% higher than the value of model prediction in 10 L bioreactor. The findings of this research could contribute to the industrial scale productions especially from lignocellulosic raw materials.
authors.affiliation: Department of Bioengineering, Faculty of Engineering, University of Ege, 35100 Bornova, Izmir, Turkey. esraimamoglu@yahoo.com
authors.forename: Esra
authors.initials: E
authors.lastname: Imamoglu
authors.forename: Fazilet Vardar
authors.initials: FV
authors.lastname: Sukan
mesh_terms: D056804:Biofuels; D019149:Bioreactors; D001709:Biotechnology / Q000379:methods*; D004926:Escherichia coli / Q000378:metabolism; D000431:Ethanol / Q000378:metabolism*; D005947:Glucose / Q000032:analysis; D007700:Kinetics; D019344:Lactic Acid / Q000096:biosynthesis; D008962:Models, Theoretical; D012275:Oryza / Q000737:chemistry; D012212:Rheology; D014866:Waste Products / Q000032:analysis; D014994:Xylose / Q000032:analysis
chemical_list: D056804:Biofuels; D014866:Waste Products; D019344:Lactic Acid; D000431:Ethanol; D014994:Xylose; D005947:Glucose
keywords: Bioethanol production; Kinetic modeling; Scale-up; Shaken flask; Stirred-tank bioreactors