title: Targeted silencing of MART-1 gene expression by RNA interference enhances the migration ability of uveal melanoma cells.
abstract: Uveal melanoma (UM) is the most common primary intraocular malignancy and the leading potentially fatal primary intraocular disease in adults. Melanoma antigen recognized by T-cells (MART-1) has been studied extensively as a clinically important diagnostic marker for melanoma, however, its biological function remains unclear. In the present study, the UM cell line SP6.5, which showed a high level of MART-1 expression, was subjected to small interfering RNA-mediated silencing of MART-1. Silencing of MART-1 expression increased the migration ability of SP6.5 cells and down-regulated the expression of the metastasis suppressor NM23. Our results suggest that MART-1 is a candidate target for the development of therapeutic strategies for UM and in particular for the suppression of metastasis associated with this malignancy.
authors.affiliation: Department of Ophthalmology, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China.
authors.forename: Yidan
authors.initials: Y
authors.lastname: Zhang
authors.forename: Renbing
authors.initials: R
authors.lastname: Jia
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.forename: Xiaofang
authors.initials: X
authors.lastname: Xu
authors.forename: Yuting
authors.initials: Y
authors.lastname: Yao
authors.forename: Shengfan
authors.initials: S
authors.lastname: Ge
authors.forename: Xianqun
authors.initials: X
authors.lastname: Fan
mesh_terms: D059447:Cell Cycle Checkpoints; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D006801:Humans; D058965:MART-1 Antigen / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D008545:Melanoma / Q000378:metabolism / Q000473:pathology; D054778:NM23 Nucleoside Diphosphate Kinases / Q000235:genetics / Q000378:metabolism; D034622:RNA Interference; D012333:RNA, Messenger / Q000378:metabolism; D034741:RNA, Small Interfering / Q000378:metabolism; D025521:Tumor Suppressor Proteins / Q000235:genetics / Q000378:metabolism; D043222:Ubiquitin Thiolesterase / Q000235:genetics / Q000378:metabolism; D014604:Uveal Neoplasms / Q000378:metabolism / Q000473:pathology; D000098943:Uveal Melanoma
chemical_list: C400134:BAP1 protein, human; D058965:MART-1 Antigen; D054778:NM23 Nucleoside Diphosphate Kinases; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D025521:Tumor Suppressor Proteins; C517938:NME1 protein, human; D043222:Ubiquitin Thiolesterase