title: The relationships between particulate and soluble alkaline phosphatase activities and the concentration of phosphorus dissolved in the seawater of Toulon Bay (NW Mediterranean).
abstract: The activities of particulate and soluble phosphatase were analyzed monthly for 1 year in the coastal ecosystems of the NW Mediterranean Sea. The mean contribution of the particulate activity increased from 56% at an MUF-P concentration of 30 μM to 77% at 0.04 μM. This particulate activity was negatively correlated with the DIP, DOP and TDP concentrations when the activities were related to the seawater volume, chlorophyll a or the protein concentration. The TDP correlations were highly significant (p: 0.001). The DOP correlations were significant (p: 0.04) and became highly significant (p: 0.009) at low DIP concentrations (<0.13 μM). The DIP correlations were significant (p: 0.04) only at low DOP concentrations (<0.18 μM). Thus, the effects of seawater DIP and DOP were found to be linked. The soluble activity exhibited distinct phosphatase fractions with high (0.5-29.5 μM) and low (0.02-2 μM) Km values, but none exhibited significant correlations with phosphorus compounds.
authors.affiliation: Université du Sud Toulon-Var, PROTEE, EA 3819, 83957 La Garde, France. Electronic address: boge@univ-tln.fr.
authors.forename: Gérard
authors.initials: G
authors.lastname: Bogé
authors.forename: Magali
authors.initials: M
authors.lastname: Lespilette
authors.forename: Dominique
authors.initials: D
authors.lastname: Jamet
authors.forename: Jean-Louis
authors.initials: JL
authors.lastname: Jamet
mesh_terms: D000469:Alkaline Phosphatase / Q000032:analysis / Q000378:metabolism*; D061006:Bays / Q000737:chemistry*; D002734:Chlorophyll / Q000032:analysis; D000077194:Chlorophyll A; D017753:Ecosystem; D004784:Environmental Monitoring; D008522:Mediterranean Sea; D010758:Phosphorus / Q000032:analysis / Q000378:metabolism*; D012623:Seawater / Q000737:chemistry*; D014874:Water Pollutants, Chemical / Q000032:analysis / Q000378:metabolism*
chemical_list: D014874:Water Pollutants, Chemical; D002734:Chlorophyll; D010758:Phosphorus; D000469:Alkaline Phosphatase; D000077194:Chlorophyll A
keywords: Alkaline phosphatase; DIP; DOP; Kinetics; MUF; MUF-P; Mediterranean Sea; Methyl umbelliferyl phosphate; Phosphorus; Plankton; SRP; TDP; dissolved inorganic phosphorus; dissolved organic phosphorus; methylumbelliferone; soluble reactive phosphorus; total dissolved phosphorus