title: Angiotensin-(1-9) enhances stasis-induced venous thrombosis in the rat because of the impairment of fibrinolysis.
abstract: BACKGROUND
  ACE2 alternatively converts angiotensin (Ang) II into Ang-(1-7) and Ang I into Ang-(1-9). There is little information in the literature with respect to Ang-(1-9) properties. A number of studies show a link between peptides of the renin-angiotensin system and thrombosis.
  
  
  METHODS
  We have investigated the influence of Ang-(1-9) on stasis-induced venous thrombosis in the rat. The contribution of coagulation and fibrinolytic systems, angiotensin receptor type 1 (AT1) and MAS receptor in the mode of Ang-(1-9) action was also determined.
  
  
  RESULTS
  Ang-(1-9) enhanced thrombosis development, decreased plasma concentration of tissue plasminogen activator and increased the level of its inhibitor (PAI-1). The action of Ang-(1-9) was reversed by selective antagonist of AT1 receptor, but not Ang-(1-7) antagonist. Ang-(1-9) did not bind to the AT1 receptor.
  
  
  CONCLUSIONS
  Ang-(1-9) enhances venous thrombosis in the rat because of the impairment of fibrinolysis. The prothrombotic effect of Ang-(1-9) is mediated by Ang II acting via the AT1 receptor.
authors.affiliation: 1Department of Pharmacodynamics, Medical University of Bialystok, Poland.
authors.forename: A
authors.initials: A
authors.lastname: Mogielnicki
authors.forename: K
authors.initials: K
authors.lastname: Kramkowski
authors.forename: J M
authors.initials: JM
authors.lastname: Hermanowicz
authors.forename: A
authors.initials: A
authors.lastname: Leszczynska
authors.forename: K
authors.initials: K
authors.lastname: Przyborowski
authors.forename: W
authors.initials: W
authors.lastname: Buczko
mesh_terms: D000803:Angiotensin I / Q000378:metabolism / Q000494:pharmacology*; D000818:Animals; D005342:Fibrinolysis / Q000187:drug effects*; D008297:Male; D010446:Peptide Fragments / Q000378:metabolism / Q000494:pharmacology*; D017395:Plasminogen Activator Inhibitor 1 / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D044140:Receptor, Angiotensin, Type 1 / Q000187:drug effects / Q000502:physiology; D010959:Tissue Plasminogen Activator / Q000378:metabolism; D020246:Venous Thrombosis / Q000139:chemically induced*
chemical_list: D010446:Peptide Fragments; D017395:Plasminogen Activator Inhibitor 1; D044140:Receptor, Angiotensin, Type 1; C506331:angiotensin I (1-9); D000803:Angiotensin I; D010959:Tissue Plasminogen Activator
keywords: Thrombosis; angiotensin 1–9; angiotensins; fibrinolysis; rat