title: Dynamic recruitment of CDK5RAP2 to centrosomes requires its association with dynein.
abstract: CDK5RAP2 is a centrosomal protein known to be involved in the regulation of the γ-tubulin ring complex and thus the organization of microtubule arrays. However, the mechanism by which CDK5RAP2 is itself recruited to centrosomes is poorly understood. We report here that CDK5RAP2 displays highly dynamic attachment to centrosomes in a microtubule-dependent manner. CDK5RAP2 associates with the retrograde transporter dynein-dynactin and contains a sequence motif that binds to dynein light chain 8. Significantly, disruption of cellular dynein-dynactin function reduces the centrosomal level of CDK5RAP2. These results reveal a key role of the dynein-dynactin complex in the dynamic recruitment of CDK5RAP2 to centrosomes.
authors.affiliation: Division of Life Science and State Key Laboratory of Molecular Neuroscience, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Jia
authors.forename: Ka-Wing
authors.initials: KW
authors.lastname: Fong
authors.forename: Yuk-Kwan
authors.initials: YK
authors.lastname: Choi
authors.forename: Siu-San
authors.initials: SS
authors.lastname: See
authors.forename: Robert Z
authors.initials: RZ
authors.lastname: Qi
mesh_terms: D018797:Cell Cycle Proteins; D018385:Centrosome / Q000378:metabolism*; D004398:Dyneins / Q000378:metabolism*; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000378:metabolism*; D008870:Microtubules / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D011485:Protein Binding
chemical_list: C405929:CDK5RAP2 protein, human; D018797:Cell Cycle Proteins; D047908:Intracellular Signaling Peptides and Proteins; D009419:Nerve Tissue Proteins; D004398:Dyneins