title: Current methods for molecular typing of Campylobacter species.
abstract: Campylobacter remains one of the most common bacterial causes of gastroenteritis worldwide. Tracking sources of this organism is challenging due to the large numbers of human cases, and the prevalence of this organism throughout the environment due to growth in a wide range of animal species. Many molecular subtyping methods have been developed to characterize Campylobacter species, but only a few are commonly used in molecular epidemiology studies. This review examines the applicability of these methods, as well as the role that emerging whole genome sequencing technologies will play in tracking sources of Campylobacter spp. infection.
authors.affiliation: Laboratory for Foodborne Zoonoses, Public Health Agency of Canada, PO Box 640, Township Rd. 9-1, Lethbridge, AB T1J 3Z4, Canada. Eduardo.Taboada@phac-aspc.gc.ca
authors.forename: Eduardo N
authors.initials: EN
authors.lastname: Taboada
authors.forename: Clifford G
authors.initials: CG
authors.lastname: Clark
authors.forename: Emma L
authors.initials: EL
authors.lastname: Sproston
authors.forename: Catherine D
authors.initials: CD
authors.lastname: Carrillo
mesh_terms: D000818:Animals; D002167:Campylobacter / Q000145:classification* / Q000235:genetics*; D005516:Food Microbiology; D006801:Humans; D017720:Molecular Epidemiology / Q000379:methods; D058889:Molecular Typing / Q000379:methods*
keywords: CGF; Campylobacter; Comparative genomic fingerprinting; HRM; MCGH; MLST; Molecular typing; Multi-locus sequence typing; NGS; PFGE; RAPD; SNPs; WGS; Whole genome sequencing; comparative genomic fingerprinting; eMLST; extended MLST; flaA restriction fragment length polymorphism analysis; flaA short variable region sequence typing; flaA-RFLP; flaA-SVR; high-resolution melting analysis; microarray comparative genomic hybridization; multi-locus sequence typing; next-generation sequencing; pulsed field gel electrophoresis; random amplification of polymorphic DNA; single nucleotide polymorphisms; whole genome sequencing