title: Antigen specific immunotherapy generates CD27(+) CD35(+) tolerogenic dendritic cells.
abstract: OBJECTIVE
  The mechanism of antigen specific immunotherapy (ASIT) remains to be further understood. The present study aims to investigate the role of ASIT in the generation of tolerogenic dendritic cells (DC) in patients with food allergy (FA).
  
  
  METHODS
  Twenty patients with egg FA and 10 healthy subjects were recruited into this study. Blood samples were obtained from each subject before and after the ASIT and analyzed by flow cytometry.
  
  
  RESULTS
  In FA patients, the frequencies of CD27(+) B cells and TGF-b(+) DCs were significantly lower than that in healthy subjects. More than 90% CD27(+) B cells also express CD35; about 90% CD27(+) CD35(+) B cells expressed TGF-b. Coculture of CD27(+) CD35(+) B cells with immature DCs generated TGF-b-expressing tolerogenic DCs; the latter was able to induce CD4(+) CD25(+) Foxp3(+) regulatory T cells.
  
  
  CONCLUSIONS
  Treatment with ASIT can induce TGF-b-expressing tolerogenic DCs in patients with FA.
authors.affiliation: Department of Gastroenterology, First Affiliated Hospital, The PLA General Hospital, Beijing 100037, China. zhiqiangwu32@163.com
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Wu
mesh_terms: D000328:Adult; D003713:Dendritic Cells / Q000276:immunology*; D003888:Desensitization, Immunologic; D005260:Female; D005434:Flow Cytometry; D005512:Food Hypersensitivity / Q000276:immunology* / Q000517:prevention & control; D006801:Humans; D007108:Immune Tolerance / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D017463:Receptors, Complement 3b / Q000276:immunology; D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7 / Q000276:immunology
chemical_list: D017463:Receptors, Complement 3b; D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7
keywords: Antigen specific immunotherapy; B cell; CD27; CD35; Food allergy