title: Muscimol inactivation of caudal fastigial nucleus and posterior interposed nucleus in monkeys with strabismus.
abstract: Previously, we showed that neurons in the supraoculomotor area (SOA), known to encode vergence angle in normal monkeys, encode the horizontal eye misalignment in strabismic monkeys. The SOA receives afferent projections from the caudal fastigial nucleus (cFN) and the posterior interposed nucleus (PIN) in the cerebellum. The objectives of the present study were to investigate the potential roles of the cFN and PIN in 1) conjugate eye movements and 2) binocular eye alignment in strabismic monkeys. We used unilateral injections of the GABAA agonist muscimol to reversibly inactivate the cFN (4 injections in exotropic monkey S1 with ≈ 4° of exotropia; 5 injections in esotropic monkey S2 with ≈ 34° of esotropia) and the PIN (3 injections in monkey S1). cFN inactivation induced horizontal saccade dysmetria in all experiments (mean 39% increase in ipsilesional saccade gain and 26% decrease in contralesional gain). Also, mean contralesional smooth-pursuit gain was decreased by 31%. cFN inactivation induced a divergent change in eye alignment in both monkeys, with exotropia increasing by an average of 9.8° in monkey S1 and esotropia decreasing by an average of 11.2° in monkey S2 (P < 0.001). Unilateral PIN inactivation in monkey S1 resulted in a mean increase in the gain of upward saccades by 13% and also induced a convergent change in eye alignment, reducing exotropia by an average of 2.7° (P < 0.001). We conclude that cFN/PIN influences on conjugate eye movements in strabismic monkeys are similar to those postulated in normal monkeys and cFN/PIN play important and complementary roles in maintaining the steady-state misalignment in strabismus.
authors.affiliation: College of Optometry, University of Houston, Houston, Texas.
authors.forename: Anand C
authors.initials: AC
authors.lastname: Joshi
authors.forename: Vallabh E
authors.initials: VE
authors.lastname: Das
mesh_terms: D000818:Animals; D002529:Cerebellar Nuclei / Q000473:pathology / Q000502:physiology*; D008253:Macaca mulatta; D009118:Muscimol / Q000633:toxicity; D012438:Saccades / Q000502:physiology*; D013285:Strabismus / Q000139:chemically induced / Q000503:physiopathology*; D015348:Vision, Binocular / Q000502:physiology
chemical_list: D009118:Muscimol
keywords: caudal fastigial nucleus; muscimol; nonhuman primates; posterior interposed nucleus; strabismus