title: Electrospun poly(L-lactide) fiber with ginsenoside rg3 for inhibiting scar hyperplasia of skin.
abstract: Hypertrophic scarring (HS) has been considered as a great concern for patients and a challenging problem for clinicians as it can be cosmetically disfiguring and functionally debilitating. In this study, Ginsenoside Rg3/Poly(l-lactide) (G-Rg3/PLLA) electrospun fibrous scaffolds covering on the full-thickness skin excisions location was designed to suppress the hypertrophic scar formation in vivo. SEM and XRD results indicated that the crystal G-Rg3 carried in PLLA electrospun fibers was in amorphous state, which facilitates the solubility of G-Rg3 in the PLLA electrospun fibrous scaffolds, and solubility of G-Rg3 in PBS is increased from 3.2 µg/ml for pure G-Rg3 powders to 19.4 µg/ml for incorporated in PLLA-10% fibers. The released G-Rg3 content in the physiological medium could be further altered from 324 to 3445 µg in a 40-day release period by adjusting the G-Rg3 incorporation amount in PLLA electrospun fibers. In vitro results demonstrated that electrospun G-Rg3/PLLA fibrous scaffold could significantly inhibit fibroblast cell growth and proliferation. In vivo results confirmed that the G-Rg3/PLLA electrospun fibrous scaffold showed significant improvements in terms of dermis layer thickness, fibroblast proliferation, collagen fibers and microvessels, revealing that the incorporation of the G-Rg3 in the fibers prevented the HS formation. The above results demonstrate the potential use of G-Rg3/PLLA electrospun fibrous scaffolds to rapidly minimize fibroblast growth and restore the structural and functional properties of wounded skin for patients with deep trauma, severe burn injury, and surgical incision.
authors.affiliation: School of Biomedical Engineering and Med-X Research Institute, Shanghai Jiao Tong University, Shanghai, PR China.
authors.forename: Wenguo
authors.initials: W
authors.lastname: Cui
authors.forename: Liying
authors.initials: L
authors.lastname: Cheng
authors.forename: Changmin
authors.initials: C
authors.lastname: Hu
authors.forename: Haiyan
authors.initials: H
authors.lastname: Li
authors.forename: Yuguang
authors.initials: Y
authors.lastname: Zhang
authors.forename: Jiang
authors.initials: J
authors.lastname: Chang
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D017439:Cicatrix, Hypertrophic / Q000517:prevention & control*; D003094:Collagen / Q000378:metabolism; D005347:Fibroblasts / Q000166:cytology; D036145:Ginsenosides / Q000737:chemistry*; D006801:Humans; D008855:Microscopy, Electron, Scanning; D011091:Polyesters / Q000737:chemistry*; D011817:Rabbits; D012995:Solubility; D054457:Tissue Scaffolds / Q000737:chemistry*; D014961:X-Ray Diffraction
chemical_list: D036145:Ginsenosides; D011091:Polyesters; C097367:ginsenoside Rg3; C033616:poly(lactide); D003094:Collagen