title: Proteomic analysis of apoptotic and oncotic pancreatic acinar AR42J cells treated with caerulein.
abstract: This study aims to determine the differentially expressed proteins in the pancreatic acinar cells undergoing apoptosis and oncosis stimulated with caerulein to explore different cell death process of the acinar cell. AR42J cells were treated with caerulein to induce cell model of acute pancreatitis. Cells that were undergoing apoptosis and oncosis were separated by flow cytometry. Then differentially expressed proteins in the two groups of separated cells were detected by shotgun liquid chromatography-tandem mass spectrometry. The results showed that 11 proteins were detected in both apoptosis group and oncosis group, 17 proteins were detected only in apoptosis group and 29 proteins were detected only in oncosis group. KEGG analysis showed that proteins detected only in apoptosis group were significantly enriched in 10 pathways, including ECM-receptor interaction, cell adhesion molecules, and proteins detected only in oncosis group were significantly enriched in three pathways, including endocytosis, base excision repair, and RNA degradation. These proteins we detected are helpful for us to understand the process of cell death in acute pancreatitis and may be useful for changing the death mode of pancreatic acinar cells, thus attenuating the severity of pancreatitis.
authors.affiliation: Department of General Surgery, The First Affiliated Hospital of Harbin Medical University, 23 You Zheng Street, Nangang District, Harbin, 150001, People's Republic of China.
authors.forename: Jiangtao
authors.initials: J
authors.lastname: Chu
authors.forename: Hongliang
authors.initials: H
authors.lastname: Ji
authors.forename: Ming
authors.initials: M
authors.lastname: Lu
authors.forename: Zhituo
authors.initials: Z
authors.lastname: Li
authors.forename: Xin
authors.initials: X
authors.lastname: Qiao
authors.forename: Bei
authors.initials: B
authors.lastname: Sun
authors.forename: Weihui
authors.initials: W
authors.lastname: Zhang
authors.forename: Dongbo
authors.initials: D
authors.lastname: Xue
mesh_terms: D061354:Acinar Cells / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology*; D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002460:Cell Line; D002108:Ceruletide / Q000494:pharmacology*; D002853:Chromatography, Liquid; D005434:Flow Cytometry; D063990:Gene Ontology; D013058:Mass Spectrometry; D058977:Molecular Sequence Annotation; D010179:Pancreas / Q000473:pathology*; D010190:Pancreatic Neoplasms / Q000378:metabolism* / Q000473:pathology*; D020543:Proteome / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D040901:Proteomics / Q000379:methods*; D051381:Rats; D060888:Real-Time Polymerase Chain Reaction; D015203:Reproducibility of Results; D012984:Software; D013997:Time Factors
chemical_list: D020543:Proteome; D002108:Ceruletide