title: Regulation of cell proliferation and migration in gallbladder cancer by zinc finger X-chromosomal protein.
abstract: Gallbladder carcinoma (GBC) is one of the mostly aggressive and fatal malignancies. However, little is known about the oncogenic genes that contributed to the development of GBC. Zinc finger X-chromosomal protein (ZFX) was a novel member of the Krueppel C2H2-type zinc-finger protein family and its down-regulation led to impaired cell growth in human laryngeal squamous cell carcinoma. Here, we aim to investigate the function of ZFX in GBC cell proliferation and migration. Loss of function analysis was performed on GBC cell line (GBC-SD) using lentivirus-mediated siRNA against ZFX. The proliferation, in vitro tumorigenesis (colony-formation) ability as well as cell migration was significantly suppressed after GBC-SD cells which were infected with ZFX-siRNA-expressing lentivirus (Lv-shZFX). Our finding suggested that ZFX promoted the growth and migration of GBC cells and could present a potential molecular target for gene therapy of GBC.
authors.affiliation: Research Institute of Biliary Tract Disease, Affiliated to Shanghai Jiao Tong University, School of Medicine, Shanghai, China.
authors.forename: Zhujun
authors.initials: Z
authors.lastname: Tan
authors.forename: Shenglai
authors.initials: S
authors.lastname: Zhang
authors.forename: Maolan
authors.initials: M
authors.lastname: Li
authors.forename: Xiangsong
authors.initials: X
authors.lastname: Wu
authors.forename: Hao
authors.initials: H
authors.lastname: Weng
authors.forename: Qian
authors.initials: Q
authors.lastname: Ding
authors.forename: Yang
authors.initials: Y
authors.lastname: Cao
authors.forename: Runfa
authors.initials: R
authors.lastname: Bao
authors.forename: Yijun
authors.initials: Y
authors.lastname: Shu
authors.forename: Jiasheng
authors.initials: J
authors.lastname: Mu
authors.forename: Qichen
authors.initials: Q
authors.lastname: Ding
authors.forename: Wenguang
authors.initials: W
authors.lastname: Wu
authors.forename: Jiahua
authors.initials: J
authors.lastname: Yang
authors.forename: Lin
authors.initials: L
authors.lastname: Zhang
authors.forename: Yingbin
authors.initials: Y
authors.lastname: Liu
mesh_terms: D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D005706:Gallbladder Neoplasms; D015870:Gene Expression; D055785:Gene Knockdown Techniques; D006801:Humans; D051741:Kruppel-Like Transcription Factors / Q000235:genetics* / Q000378:metabolism*; D020778:Matrix Metalloproteinase 2 / Q000235:genetics / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000235:genetics / Q000378:metabolism; D034741:RNA, Small Interfering / Q000235:genetics
chemical_list: D051741:Kruppel-Like Transcription Factors; D034741:RNA, Small Interfering; C062076:zinc finger protein, X-linked; C522361:MMP2 protein, human; D020778:Matrix Metalloproteinase 2; C579270:MMP9 protein, human; D020780:Matrix Metalloproteinase 9
keywords: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; DMEM; Dulbecco's modified Eagle medium; EGFP; Enhanced green fluorescent protein; FBS; Fetal calf serum; GBC; Gallbladder carcinoma; HCC; Hepatocellular carcinoma; KCMF1; Lv-shZFX; MMP; MMP-2; MMP-9; MOI; MTT; Matrix metallopeptidase 2; Matrix metallopeptidase 9; Matrix metalloproteinase; Messenger RNA; Migration; Multiplicities of infection; PCR; Polymerase chain reaction; Potassium channel modulatory factor 1; Proliferation; Short hairpin RNAs; Small interfering RNA; ZBTB20; ZFX; ZFX-siRNA-expressing lentivirus; ZNF; ZNF267; Zinc finger; Zinc finger X-chromosomal protein; Zinc finger and BTB domain containing 20; Zinc finger protein 267; mRNA; shRNAs; siRNA