title: Conformational switch for cisplatin with hemin/G-quadruplex DNAzyme supersandwich structure.
abstract: Cisplatin is a representative of cytotoxic and antineoplastic metallodrugs used for the treatment of various malignancies. In this paper, an enzyme-free amplification platform involving an autonomous target triggered process that yields the formation of the hemin/G-quadruplex DNAzyme wires was designed for the detection of cisplatin. Given the virtue of hemin/G-quadruplex DNAzyme wires (supersandwich DNAzyme structure) containing many units of hemin/G-quadruplex DNAzyme, an amplified electrochemical signal was achieved. Based on the combination of cisplatin with the guanine of the designed hemin/G-quadruplex DNAzyme supersandwich DNA structure, changing this structure and decreasing its catalytic effect on H2O2, it was used for the study on the analysis of cisplatin. With the concentration of the cisplatin increasing, the conformational change of the supersandwich DNA structure changed gradually. Then a relationship between the concentration of the cisplatin and the obtained electrochemical signal can be established. The detection concentration range of cisplatin was from 0.05 to 5 μM with a low detection limit of 20 nM using a signal three-fold the background noise and the correlation coefficient is 0.9993. The proposed approach is able to provide unique opportunities as simplicity in design and easy operations. Therefore, the proposed sensor presents remarkably high sensitivity and selectivity.
authors.affiliation: Key Laboratory of Chem-Biosensing, Anhui Province, Anhui Normal University, Wuhu 241000, PR China; Key Laboratory of Functional Molecular Solids, Anhui Province, Anhui Normal University, Wuhu 241000, PR China; College of Chemistry and Materials Science, Anhui Normal University, Wuhu 241000, PR China.
authors.forename: Guangfeng
authors.initials: G
authors.lastname: Wang
authors.forename: Xiuping
authors.initials: X
authors.lastname: He
authors.forename: Ling
authors.initials: L
authors.lastname: Chen
authors.forename: Yanhong
authors.initials: Y
authors.lastname: Zhu
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Zhang
authors.forename: Lun
authors.initials: L
authors.lastname: Wang
mesh_terms: D000970:Antineoplastic Agents / Q000032:analysis*; D015374:Biosensing Techniques / Q000379:methods*; D002945:Cisplatin / Q000032:analysis*; D021881:DNA, Catalytic / Q000737:chemistry*; D055664:Electrochemical Techniques / Q000379:methods; D054856:G-Quadruplexes; D006427:Hemin / Q000737:chemistry*; D057230:Limit of Detection
chemical_list: D000970:Antineoplastic Agents; D021881:DNA, Catalytic; D006427:Hemin; D002945:Cisplatin
keywords: Biosensor; Cisplatin; Hemin/G-quadruplex DNAzyme; Supersandwich DNA structure