title: Mass fabrication and delivery of 3D multilayer μTags into living cells.
abstract: Continuous monitoring of in vivo biological processes and their evolution at the cellular level would enable major advances in our understanding of biology and disease. As a stepping stone towards chronic cellular monitoring, we demonstrate massively parallel fabrication and delivery of 3D multilayer micro-Tags (μTags) into living cells. Both 10 μm × 10 μm × 1.5 μm and 18 μm × 7 μm × 1.5 μm devices containing inductive and capacitive structures were designed and fabricated as potential passive radio-frequency identification tags. We show cellular internalization and persistence of μTags over a 5-day period. Our results represent a promising advance in technologies for studying biology and disease at the cellular level.
authors.affiliation: Department of Electrical Engineering, Stanford University, Stanford, CA 94305, USA. lisachen@alumni.stanford.edu
authors.forename: Lisa Y
authors.initials: LY
authors.lastname: Chen
authors.forename: Kokab B
authors.initials: KB
authors.lastname: Parizi
authors.forename: Hisanori
authors.initials: H
authors.lastname: Kosuge
authors.forename: Kaveh M
authors.initials: KM
authors.lastname: Milaninia
authors.forename: Michael V
authors.initials: MV
authors.lastname: McConnell
authors.forename: H-S Philip
authors.initials: HS
authors.lastname: Wong
authors.forename: Ada S Y
authors.initials: AS
authors.lastname: Poon
mesh_terms: D000818:Animals; D040761:Biomimetic Materials; D002460:Cell Line; D004581:Electronics / Q000295:instrumentation; D008264:Macrophages / Q000378:metabolism; D008422:Materials Testing; D051379:Mice; D055616:Microtechnology / Q000379:methods*; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D012825:Silicon / Q000737:chemistry
chemical_list: D012825:Silicon