title: Structure characterization and immunocompetence of a glucan from the fruiting bodies of Cantharellus cibarius.
abstract: A protein-bound polysaccharide fraction (JBP-1) was obtained from the fruiting bodies of Cantharellus cibarius. Its chemical composition was studied by the cooperative usage of multiple chemical and spectral methods and characterized to be a fraction with a molecular weight of 4.8 × 10(5) Da and only composed of glucose. Methylation analysis revealed that the sugar residues in JBP-1 are existing as t-, 1,6-, and 1,3,6-linked Glcp sugar residues. The immunocompetence of the fraction was evaluated with the proliferation assay of mouse splenocytes, and the result revealed that JBP-1 could significantly stimulate the proliferation of mouse splenocytes in a dose-dependent manner, with p < 0.001 at the concentration of 100 μg/ml and 30 μg/ml, p < 0.05 at 10 μg/ml. These results give us a primary scientific evidence to further explore the pharmaceutical function of this mushroom.
authors.affiliation: a State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University Health Science Center , Beijing 100191 China.
authors.forename: Xiao-Qiang
authors.initials: XQ
authors.lastname: Han
authors.forename: Wei-Ji
authors.initials: WJ
authors.lastname: Li
authors.forename: Chun-Hay
authors.initials: CH
authors.lastname: Ko
authors.forename: Xiao-Ming
authors.initials: XM
authors.lastname: Gao
authors.forename: Chun-Xia
authors.initials: CX
authors.lastname: Han
authors.forename: Peng-Fei
authors.initials: PF
authors.lastname: Tu
mesh_terms: D000363:Agaricales / Q000737:chemistry*; D000596:Amino Acids / Q000032:analysis*; D000818:Animals; D004305:Dose-Response Relationship, Drug; D048690:Fruiting Bodies, Fungal / Q000737:chemistry; D005936:Glucans / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification* / Q000494:pharmacology; D005947:Glucose / Q000032:analysis; D007121:Immunocompetence; D051379:Mice; D015394:Molecular Structure; D008970:Molecular Weight; D019906:Nuclear Magnetic Resonance, Biomolecular; D013154:Spleen / Q000166:cytology* / Q000187:drug effects / Q000276:immunology
chemical_list: D000596:Amino Acids; D005936:Glucans; D005947:Glucose