title: A new role for RINT-1 in SNARE complex assembly at the trans-Golgi network in coordination with the COG complex.
abstract: Docking and fusion of transport vesicles/carriers with the target membrane involve a tethering factor-mediated initial contact followed by soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE)-catalyzed membrane fusion. The multisubunit tethering CATCHR family complexes (Dsl1, COG, exocyst, and GARP complexes) share very low sequence homology among subunits despite likely evolving from a common ancestor and participate in fundamentally different membrane trafficking pathways. Yeast Tip20, as a subunit of the Dsl1 complex, has been implicated in retrograde transport from the Golgi apparatus to the endoplasmic reticulum. Our previous study showed that RINT-1, the mammalian counterpart of yeast Tip20, mediates the association of ZW10 (mammalian Dsl1) with endoplasmic reticulum-localized SNARE proteins. In the present study, we show that RINT-1 is also required for endosome-to-trans-Golgi network trafficking. RINT-1 uncomplexed with ZW10 interacts with the COG complex, another member of the CATCHR family complex, and regulates SNARE complex assembly at the trans-Golgi network. This additional role for RINT-1 may in part reflect adaptation to the demand for more diverse transport routes from endosomes to the trans-Golgi network in mammals compared with those in a unicellular organism, yeast. The present findings highlight a new role of RINT-1 in coordination with the COG complex.
authors.affiliation: School of Life Sciences, Tokyo University of Pharmacy and Life Sciences, Hachioji, Tokyo 192-0392, Japan Division of Oral Biochemistry, Niigata University Graduate School of Medical and Dental Sciences, 2-5274 Gakkocho-dori, Chuo-ku, Niigata 951-8514, Japan Department of Cell Biology, Fukuoka University School of Medicine, Jonan-ku, Fukuoka 814-0180, Japan.
authors.forename: Kohei
authors.initials: K
authors.lastname: Arasaki
authors.forename: Daichi
authors.initials: D
authors.lastname: Takagi
authors.forename: Akiko
authors.initials: A
authors.lastname: Furuno
authors.forename: Miwa
authors.initials: M
authors.lastname: Sohda
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Misumi
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Wakana
authors.forename: Hiroki
authors.initials: H
authors.lastname: Inoue
authors.forename: Mitsuo
authors.initials: M
authors.lastname: Tagaya
mesh_terms: D018797:Cell Cycle Proteins / Q000378:metabolism*; D002772:Cholera Toxin / Q000378:metabolism; D002868:Chromosomal Proteins, Non-Histone / Q000378:metabolism; D004721:Endoplasmic Reticulum / Q000378:metabolism; D011992:Endosomes / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D008954:Models, Biological; D046912:Multiprotein Complexes / Q000378:metabolism*; D017434:Protein Structure, Tertiary; D021381:Protein Transport; D050600:SNARE Proteins / Q000378:metabolism*; D051438:Syntaxin 16 / Q000378:metabolism; D021601:trans-Golgi Network / Q000378:metabolism*
chemical_list: D018797:Cell Cycle Proteins; D002868:Chromosomal Proteins, Non-Histone; D008869:Microtubule-Associated Proteins; D046912:Multiprotein Complexes; C422909:RINT1 protein, human; D050600:SNARE Proteins; D051438:Syntaxin 16; C488261:ZW10 protein, human; D002772:Cholera Toxin