title: Intestinal absorption of lysozyme, an egg-white allergen, in rats: kinetics and effect of NSAIDs.
abstract: The absorption pathway(s) of a representative food allergen, lysozyme, and the mechanisms of lysozyme absorption facilitated by non-steroidal anti-inflammatory drugs were examined by intestinal closed-loop and re-circulating perfusion methods in rats. The absorption rate of fluorescein isothiocyanate (FITC)-labeled lysozyme in the proximal intestine was higher than that for a marker of non-specific absorption, FD-10, and was suppressed by colchicine (endocytosis inhibitor). Aspirin increased the absorption of FITC-lysozyme in the proximal intestine with no effects on tissue accumulation. Diclofenac facilitated FITC-lysozyme absorption, but meloxicam and loxoprofen exerted no effects on absorption. Co-administration of misoprostol (synthetic prostaglandin-E1 analog) with aspirin significantly ameliorated the aspirin-facilitated absorption of FITC-lysozyme to the same level as that seen with controls. Thus, lysozyme absorption was mediated by endocytic and paracellular pathways in the proximal intestine, and was facilitated by aspirin and diclofenac after impairment of the paracellular pathway. Misoprostol may suppress the allergen absorption facilitated by aspirin.
authors.affiliation: Department of Pathophysiology and Therapeutics, Institute of Biomedical and Health Sciences, Hiroshima University, Hiroshima, Japan.
authors.forename: Tomoharu
authors.initials: T
authors.lastname: Yokooji
authors.forename: Koh
authors.initials: K
authors.lastname: Hamura
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Matsuo
mesh_terms: D000485:Allergens / Q000493:pharmacokinetics*; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000008:administration & dosage*; D001241:Aspirin / Q000008:administration & dosage; D004008:Diclofenac / Q000008:administration & dosage; D004347:Drug Interactions; D004527:Egg Proteins / Q000493:pharmacokinetics*; D007408:Intestinal Absorption / Q000187:drug effects* / Q000502:physiology*; D008297:Male; D008657:Metabolic Clearance Rate / Q000187:drug effects / Q000502:physiology; D016595:Misoprostol / Q000008:administration & dosage; D009113:Muramidase / Q000493:pharmacokinetics*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D000485:Allergens; D000894:Anti-Inflammatory Agents, Non-Steroidal; D004527:Egg Proteins; D016595:Misoprostol; D004008:Diclofenac; D009113:Muramidase; D001241:Aspirin
keywords: AUC; Absorption; CBB; COX; DMSO; Endocytosis; FD; FDEIA; FITC; Ig; Lysozyme; NSAIDs; Non-steroidal anti-inflammatory drugs; PBS; Paracellular; SDS–PAGE; area under the plasma concentration–time curve; coomassie brilliant blue; cyclooxygenase; dimethyl sulfoxide; fluorescein isothiocyanate; fluorescein isothiocyanate-labeled dextran; food-dependent exercise-induced anaphylaxis; immunoglobulin; non-steroidal anti-inflammatory drugs; phosphate-buffered saline; sodium dodecyl sulfate–polyacrylamide gel electrophoresis