title: Production of omega-3 eicosapentaenoic acid by metabolic engineering of Yarrowia lipolytica.
abstract: The availability of the omega-3 fatty acids eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) is currently limited because they are produced mainly by marine fisheries that cannot keep pace with the demands of the growing market for these products. A sustainable non-animal source of EPA and DHA is needed. Metabolic engineering of the oleaginous yeast Yarrowia lipolytica resulted in a strain that produced EPA at 15% of dry cell weight. The engineered yeast lipid comprises EPA at 56.6% and saturated fatty acids at less than 5% by weight, which are the highest and the lowest percentages, respectively, among known EPA sources. Inactivation of the peroxisome biogenesis gene PEX10 was crucial in obtaining high EPA yields and may increase the yields of other commercially desirable lipid-related products. This technology platform enables the production of lipids with tailored fatty acid compositions and provides a sustainable source of EPA.
authors.affiliation: Industrial Biosciences, E.I. du Pont de Nemours and Company, Wilmington, Delaware, USA.
authors.forename: Zhixiong
authors.initials: Z
authors.lastname: Xue
authors.forename: Pamela L
authors.initials: PL
authors.lastname: Sharpe
authors.forename: Seung-Pyo
authors.initials: SP
authors.lastname: Hong
authors.forename: Narendra S
authors.initials: NS
authors.lastname: Yadav
authors.forename: Dongming
authors.initials: D
authors.lastname: Xie
authors.forename: David R
authors.initials: DR
authors.lastname: Short
authors.forename: Howard G
authors.initials: HG
authors.lastname: Damude
authors.forename: Ross A
authors.initials: RA
authors.lastname: Rupert
authors.forename: John E
authors.initials: JE
authors.lastname: Seip
authors.forename: Jamie
authors.initials: J
authors.lastname: Wang
authors.forename: Dana W
authors.initials: DW
authors.lastname: Pollak
authors.forename: Michael W
authors.initials: MW
authors.lastname: Bostick
authors.forename: Melissa D
authors.initials: MD
authors.lastname: Bosak
authors.forename: Daniel J
authors.initials: DJ
authors.lastname: Macool
authors.forename: Dieter H
authors.initials: DH
authors.lastname: Hollerbach
authors.forename: Hongxiang
authors.initials: H
authors.lastname: Zhang
authors.forename: Dennis M
authors.initials: DM
authors.lastname: Arcilla
authors.forename: Sidney A
authors.initials: SA
authors.lastname: Bledsoe
authors.forename: Kevin
authors.initials: K
authors.lastname: Croker
authors.forename: Elizabeth F
authors.initials: EF
authors.lastname: McCord
authors.forename: Bjorn D
authors.initials: BD
authors.lastname: Tyreus
authors.forename: Ethel N
authors.initials: EN
authors.lastname: Jackson
authors.forename: Quinn
authors.initials: Q
authors.lastname: Zhu
mesh_terms: D004281:Docosahexaenoic Acids / Q000378:metabolism; D015118:Eicosapentaenoic Acid / Q000378:metabolism*; D015525:Fatty Acids, Omega-3 / Q000235:genetics* / Q000378:metabolism; D050356:Lipid Metabolism; D008055:Lipids / Q000235:genetics; D060847:Metabolic Engineering; D025062:Yarrowia / Q000235:genetics / Q000378:metabolism
chemical_list: D015525:Fatty Acids, Omega-3; D008055:Lipids; D004281:Docosahexaenoic Acids; D015118:Eicosapentaenoic Acid