title: Evaluation of the Gram stain of sputa from cystic fibrosis patients to predict the presence of Staphylococcus aureus.
abstract: We evaluated the presence of Gram-positive cocci in cluster, seen in Gram-stained smears of cystic fibrosis (CF) sputa versus the microbiological culture for the prediction of the presence of S. aureus. Gram stain provided low accuracy (69.2%; odds ratio, 4.8; 95% confidence interval, 61.3-76.1) for predicting S. aureus in CF sputum.
authors.affiliation: Microbiology unit, Hospital Mãe de Deus, Porto Alegre - RS, 90880-480, Brazil. Electronic address: leandro.reus@gmail.com.
authors.forename: Leandro Reus Rodrigues
authors.initials: LR
authors.lastname: Perez
authors.forename: Cícero Armídio Gomes
authors.initials: CA
authors.lastname: Dias
mesh_terms: D001431:Bacteriological Techniques / Q000379:methods*; D003550:Cystic Fibrosis / Q000382:microbiology*; D005840:Gentian Violet / Q000737:chemistry*; D006801:Humans; D016017:Odds Ratio; D010619:Phenazines / Q000737:chemistry*; D012680:Sensitivity and Specificity; D013183:Sputum / Q000382:microbiology*; D013211:Staphylococcus aureus / Q000145:classification / Q000302:isolation & purification*
chemical_list: C035863:Gram's stain; D010619:Phenazines; D005840:Gentian Violet
keywords: Cystic fibrosis; Gram stain; Staphylococcus aureus