title: Spectral and redox properties of the GFP synthetic chromophores as a function of pH in buffered media.
abstract: The effect of pH on the spectral and redox properties of model GFP synthetic chromophores (Cros) in a universal buffer system was investigated. The Cro hydroxy-derivatives demonstrated a Nernst-type electrochemical dependence of the anodic potential within the pH 2-8 range. Analogous studies on various fluorescent proteins were unsuccessful.
authors.affiliation: School of Chemistry and Biochemistry, Georgia Institute of Technology, 901 Atlantic Drive, Atlanta, GA 30332-0400, USA.
authors.forename: Joseph E
authors.initials: JE
authors.lastname: Reynolds
authors.forename: Mira
authors.initials: M
authors.lastname: Josowicz
authors.forename: Phillip
authors.initials: P
authors.lastname: Tyler
authors.forename: Russell B
authors.initials: RB
authors.lastname: Vegh
authors.forename: Kyril M
authors.initials: KM
authors.lastname: Solntsev
mesh_terms: D002021:Buffers; D004583:Electrons; D049452:Green Fluorescent Proteins / Q000737:chemistry* / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D010084:Oxidation-Reduction; D014867:Water / Q000737:chemistry
chemical_list: D002021:Buffers; D014867:Water; D049452:Green Fluorescent Proteins