title: Enhancement of the sensitivity of surface plasmon resonance biosensor by modifying the metal surface.
abstract: We report on the enhancement of sensitivity of SPR biosensor by modifying the metal surface. A mixture layer, in which gold and dielectric medium coexist, was simply prepared by increasing the roughness of gold surface deposited onto a glass substrate via the reduction reaction. The absorbance spectrum and atomic force microscopy study showed that the average roughness of modified substrate increased by 4 times, compared to the unmodified substrate. The SPR curves of the unmodified substrate and the modified substrate were measured with the various refractive index of the dielectric medium. The sensitivity of modified substrate to the refractive index of dielectric medium was increased by 1.5 times, compared to the unmodified substrate. In result, the addition of the mixture layer between the gold surface and the dielectric medium by the reduction reaction can provides the enhancement of sensitivity in the SPR biosensor.
authors.affiliation: Korea Electrotechnology Research Institute, 111 Hanggaul-ro, Ansan, 426-170, Republic of Korea. kimbym@keri.re.kr
authors.forename: Young Min
authors.initials: YM
authors.lastname: Bae
authors.forename: Seo Young
authors.initials: SY
authors.lastname: Son
authors.forename: Kyeong-Hee
authors.initials: KH
authors.lastname: Lee
authors.forename: Kibok
authors.initials: K
authors.lastname: Kim
authors.forename: Seung Kwon
authors.initials: SK
authors.lastname: Seol
authors.forename: Daeho
authors.initials: D
authors.lastname: Kim
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006046:Gold / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure*; D010316:Particle Size; D012031:Refractometry / Q000295:instrumentation*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D020349:Surface Plasmon Resonance / Q000295:instrumentation*; D013499:Surface Properties
chemical_list: D006046:Gold