title: Eccrine sweat contains IL-1α, IL-1β and IL-31 and activates epidermal keratinocytes as a danger signal.
abstract: Eccrine sweat is secreted onto the skin's surface and is not harmful to normal skin, but can exacerbate eczematous lesions in atopic dermatitis. Although eccrine sweat contains a number of minerals, proteins, and proteolytic enzymes, how it causes skin inflammation is not clear. We hypothesized that it stimulates keratinocytes directly, as a danger signal. Eccrine sweat was collected from the arms of healthy volunteers after exercise, and levels of proinflammatory cytokines in the sweat were quantified by ELISA. We detected the presence of IL-1α, IL-1β, and high levels of IL-31 in sweat samples. To investigate whether sweat activates keratinocytes, normal human keratinocytes were stimulated with concentrated sweat. Western blot analysis demonstrated the activation of NF-κB, ERK, and JNK signaling in sweat-stimulated keratinocytes. Real-time PCR using total RNA and ELISA analysis of supernatants showed the upregulation of IL-8 and IL-1β by sweat. Furthermore, pretreatment with IL-1R antagonist blocked sweat-stimulated cytokine production and signal activation, indicating that bioactive IL-1 is a major factor in the activation of keratinocytes by sweat. Moreover, IL-31 seems to be another sweat stimulator that activates keratinocytes to produce inflammatory cytokine, CCL2. Sweat is secreted onto the skin's surface and does not come into contact with keratinocytes in normal skin. However, in skin with a defective cutaneous barrier, such as atopic dermatitis-affected skin, sweat cytokines can directly act on epidermal keratinocytes, resulting in their activation. In conclusion, eccrine sweat contains proinflammatory cytokines, IL-1 and IL-31, and activates epidermal keratinocytes as a danger signal.
authors.affiliation: Department of Dermatology, Ehime University Graduate School of Medicine, Toon, Ehime, Japan. daixiuju@m.ehime-u.ac.jp
authors.forename: Xiuju
authors.initials: X
authors.lastname: Dai
authors.forename: Hidenori
authors.initials: H
authors.lastname: Okazaki
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Hanakawa
authors.forename: Masamoto
authors.initials: M
authors.lastname: Murakami
authors.forename: Mikiko
authors.initials: M
authors.lastname: Tohyama
authors.forename: Yuji
authors.initials: Y
authors.lastname: Shirakata
authors.forename: Koji
authors.initials: K
authors.lastname: Sayama
mesh_terms: D000328:Adult; D002478:Cells, Cultured; D018932:Chemokine CCL2 / Q000378:metabolism; D004439:Eccrine Glands / Q000378:metabolism*; D005260:Female; D064368:Healthy Volunteers; D006801:Humans; D053582:Interleukin-1alpha / Q000378:metabolism*; D053583:Interleukin-1beta / Q000378:metabolism*; D007378:Interleukins / Q000378:metabolism*; D015603:Keratinocytes / Q000378:metabolism*; D020935:MAP Kinase Signaling System; D008297:Male; D016328:NF-kappa B / Q000378:metabolism; D015398:Signal Transduction; D012867:Skin / Q000378:metabolism*; D013542:Sweat / Q000378:metabolism*; D055815:Young Adult
chemical_list: C111835:CCL2 protein, human; D018932:Chemokine CCL2; C502187:IL31 protein, human; D053582:Interleukin-1alpha; D053583:Interleukin-1beta; D007378:Interleukins; D016328:NF-kappa B