title: Rapid and sensitive determination of propofol glucuronide in hair by liquid chromatography and tandem mass spectrometry.
abstract: A fast, sensitive and selective liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the detection and quantitation of propofol glucuronide in human hair has been developed and validated. Propofol glucuronide was extracted from 10mg of hair using a simple methanol extraction method, with recovery greater than 91% at 3 quality control samples (15, 100, 4000 pg/mg). A reversed phase column (C8) was used to analyze and the mobile phase was composed of ammonium formate and acetonitrile gradient at a flow rate of 0.2 mL/min. The lower limit of quantitation (LLOQ) was 5 pg/mg and the assay was linear to 5000 pg/mg. The intra- and inter-day precision (% CV, coefficient of variation) ranged from 1.26 to 4.50% while the accuracy (% RE, relative error) were -4.24 to 4.4%. The matrix effects were monitored at 3 different concentrations and the %CV of the results for these concentrations was less than 10.6%. Propofol glucuronide was stable during processing and analysis in human hair. The procedure was validated and applied to the analysis of hair samples in human subjects previously administered in propofol.
authors.affiliation: Forensic Chemistry Laboratory, Forensic Science Division, Supreme Prosecutors' Office, Seoul, Republic of Korea. hskjay@spo.go.kr
authors.forename: Hee Seung
authors.initials: HS
authors.lastname: Kim
authors.forename: Jae Chul
authors.initials: JC
authors.lastname: Cheong
authors.forename: Jae Il
authors.initials: JI
authors.lastname: Lee
authors.forename: Moon Kyo
authors.initials: MK
authors.lastname: In
mesh_terms: D000328:Adult; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D020719:Glucuronides / Q000032:analysis*; D006197:Hair / Q000737:chemistry; D006801:Humans; D008297:Male; D015742:Propofol / Q000378:metabolism*; D053719:Tandem Mass Spectrometry / Q000379:methods*; D013997:Time Factors
chemical_list: D020719:Glucuronides; D015742:Propofol
keywords: Human hair; LC–MS/MS; Methanol extraction; Micro pulverization; Propofol glucuronide