title: Factors influencing measurement of serum iron concentration in dogs: diurnal variation and hyperferritinemia.
abstract: We evaluated diurnal variation and hyperferritinemia as factors that influence the values of serum iron concentration in dogs, using the International Committee for Standardization in Hematology (ICSH) colorimetric method. Serum iron levels were significantly higher in the morning than in the evening in 6 clinically healthy beagle dogs, and the maximum decrease in serum iron concentration was 47.3%. Moreover, the change in serum iron concentrations in 22 clinical canine cases with various serum ferritin levels was evaluated by immunoprecipitation of ferritin. The rate of decline in the serum iron concentrations positively correlated with serum ferritin levels (r=0.48, P=0.024). These results show that it is necessary to consider the sampling time and serum ferritin level for accurate interpretation of serum iron concentrations in dogs.
authors.affiliation: Department of Small Animal Internal Medicine, School of Veterinary Medicine, Kitasato University, 23-35-1 Higashi, Towada, Aomori 034-8628, Japan.
authors.forename: Seishiro
authors.initials: S
authors.lastname: Chikazawa
authors.forename: Yasutomo
authors.initials: Y
authors.lastname: Hori
authors.forename: Kazutaka
authors.initials: K
authors.lastname: Kanai
authors.forename: Naoyuki
authors.initials: N
authors.lastname: Ito
authors.forename: Fumio
authors.initials: F
authors.lastname: Hoshi
authors.forename: Koichi
authors.initials: K
authors.lastname: Orino
authors.forename: Kiyotaka
authors.initials: K
authors.lastname: Watanabe
authors.forename: Seiichi
authors.initials: S
authors.lastname: Higuchi
mesh_terms: D000818:Animals; D002940:Circadian Rhythm / Q000502:physiology*; D003124:Colorimetry / Q000662:veterinary; D004285:Dogs; D005260:Female; D005293:Ferritins / Q000097:blood*; D047468:Immunoprecipitation / Q000662:veterinary; D007501:Iron / Q000097:blood*; D008297:Male; D012016:Reference Values
chemical_list: D005293:Ferritins; D007501:Iron