title: Optimization protocols and improved strategies of cross-linked enzyme aggregates technology: current development and future challenges.
abstract: Cross-linked enzyme aggregate (CLEA) technology has been regarded as an effective carrier-free immobilization method. This method is very attractive due to its simplicity and robustness, as well as for the possibility of using the crude enzyme extract and the opportunity to co-immobilize multiple different enzymes. The resulting CLEAs generally exhibit high catalyst productivities, improved storage and operational stability and are easy to recycle. Nowadays, although the technology has been applied to various enzymes, some undesirable properties have limited its further application. To overcome these limitations, novel strategies have been developing in recent years. This mini-review focuses on process optimization, new improved strategies and the latest advances on CLEAs technology.
authors.affiliation: Research Center for Fermentation Engineering of Hebei, College of Bioscience and Bioengineering, Hebei University of Science and Technology , Shijiazhang , PR China .
authors.forename: Jian Dong
authors.initials: JD
authors.lastname: Cui
authors.forename: Shi Ru
authors.initials: SR
authors.lastname: Jia
mesh_terms: D055162:Biocatalysis; D001709:Biotechnology / Q000379:methods*; D003432:Cross-Linking Reagents / Q000737:chemistry; D004800:Enzymes, Immobilized / Q000737:chemistry*
chemical_list: D003432:Cross-Linking Reagents; D004800:Enzymes, Immobilized
keywords: Biocatalysis; cross-linked enzyme aggregates; enzyme immobilization; improved strategies; process optimization