title: Metabonomic profiling in studying anti-osteoporosis effects of strontium fructose 1,6-diphosphate on estrogen deficiency-induced osteoporosis in rats by GC/TOF-MS.
abstract: A novel strontium salt compound strontium fructose 1, 6-diphosphate (FDP-Sr) has been proved to have highly effective for bone loss via dual effects of stimulating bone formation and suppressing bone absorption. In the present study, metabolomic approach was used to identify and study potential biomarkers associated with the effect and safety of FDP-Sr. The metabolomic profiles of bone loss induced by estrogen deficiency in a rat model was described to attain a system-level map of the shift on the metabolic response in plasma using GC/TOF-MS, after FDP-Sr was orally administered at the dose of 110 mg/kg/day for the prevention and 220 mg/kg/day for the treatment. Meanwhile, bone turnover biomarkers and bone mineral density were investigated to identify the specific changes of potential anti-osteoporosis effects of FDP-Sr. The differences in metabolic profiles between osteoporosis rats and FDP-Sr treated rats were well observed by the partial least squares-discriminant analysis (PLS-DA) to the MS spectra. Some metabolites including homocysteine, arachidonic acid, alanine, and hydroxyproline, which significantly changed during osteoporosis progression could be effectively reversed after FDP-Sr therapy. Of course some metabolites such as uric acid, glyceric acid, octadecadienoic acid, docosahexaenoic acid, oleic acid, and hexadecanoic acid were not found to reverse significantly after FDP-Sr administration. These results delineated the FDP-Sr effects-related metabolic alterations in the bone loss rats, suggesting that metabonomic analysis could provide helpful information on the new potential biomarkers relating to the mechanism of anti-osteoporosis action and side effects of FDP-Sr against estrogen deficiency induced bone loss.
authors.affiliation: School of Pharmaceutical Sciences, Nanjing University of Technology, Nanjing 210009, People's Republic of China.
authors.forename: Bo
authors.initials: B
authors.lastname: Ma
authors.forename: Xiaotian
authors.initials: X
authors.lastname: Li
authors.forename: Qi
authors.initials: Q
authors.lastname: Zhang
authors.forename: Di
authors.initials: D
authors.lastname: Wu
authors.forename: Guangji
authors.initials: G
authors.lastname: Wang
authors.forename: Jiye
authors.initials: J
authors.lastname: A
authors.forename: Jianguo
authors.initials: J
authors.lastname: Sun
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.forename: Yinhui
authors.initials: Y
authors.lastname: Liu
authors.forename: Yonglu
authors.initials: Y
authors.lastname: Wang
authors.forename: Hanjie
authors.initials: H
authors.lastname: Ying
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000097:blood / Q000378:metabolism; D001835:Body Weight / Q000187:drug effects; D015519:Bone Density / Q000187:drug effects; D004967:Estrogens / Q000172:deficiency*; D005260:Female; D005635:Fructosediphosphates / Q000494:pharmacology* / Q000627:therapeutic use; D008401:Gas Chromatography-Mass Spectrometry; D055432:Metabolomics; D009929:Organ Size / Q000187:drug effects; D010024:Osteoporosis / Q000097:blood / Q000188:drug therapy* / Q000378:metabolism* / Q000503:physiopathology; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D015415:Biomarkers; D004967:Estrogens; D005635:Fructosediphosphates; C029063:fructose-1,6-diphosphate
keywords: 6-diphosphate (FDP-Sr); GC/TOF-MS; Metabonomic; Osteoporosis; Strontium fructose 1