title: Cytogenetic and molecular analysis of infertile Chinese men: karyotypic abnormalities, Y-chromosome microdeletions, and CAG and GGN repeat polymorphisms in the androgen receptor gene.
abstract: Chromosome abnormalities, Y-chromosome microdeletions, and androgen receptor gene CAG and GGN repeat polymorphisms in infertile Chinese men featuring severe oligospermia and azoospermia were analyzed. Ninety-six fertile men and 189 non-obstructive infertile men, including 125 patients with azoospermia and 64 with severe oligozoospermia, were studied. Seventeen infertile men (9.0%) carried a chromosome abnormality. Twenty (10.6%) carried a Y-chromosome microdeletion. In the remainder of the patients and controls, GGN and CAG repeats were sequenced. Short GGN repeats (n < 23) appeared to be associated with defective spermatogenesis, with the number of GGN repeats strongly correlated with sperm counts. No significant difference in CAG repeats was found between patients and controls, nor were CAG repeats correlated with sperm counts. However, for CAG repeats ranging between 24 and 25, there was a >2.5-fold risk (OR = 2.539, 95%CI = 1.206-5.344, P < 0.05) of severe oligospermia and azoospermia. Our results confirmed the significant role of chromosome abnormalities, Y-chromosome microdeletions, and GGN repeats in Chinese male infertility.
authors.affiliation: Key Laboratory of Bio-Resources and Eco-Environment, Institute of Medical Genetics, Ministry of Education, College of Life Science, Sichuan University, Chengdu, China. han578388@gmail.com
authors.forename: T T
authors.initials: TT
authors.lastname: Han
authors.forename: J
authors.initials: J
authors.lastname: Ran
authors.forename: X P
authors.initials: XP
authors.lastname: Ding
authors.forename: L J
authors.initials: LJ
authors.lastname: Li
authors.forename: L Y
authors.initials: LY
authors.lastname: Zhang
authors.forename: Y P
authors.initials: YP
authors.lastname: Zhang
authors.forename: S S
authors.initials: SS
authors.lastname: Nie
authors.forename: L
authors.initials: L
authors.lastname: Chen
mesh_terms: D059786:Abnormal Karyotype; D000328:Adult; D053713:Azoospermia / Q000235:genetics*; D016022:Case-Control Studies; D002681:China; D002872:Chromosome Deletion; D041322:Chromosomes, Human, Y; D056915:DNA Copy Number Variations; D006801:Humans; D007248:Infertility, Male; D008297:Male; D018895:Microsatellite Repeats; D008875:Middle Aged; D009845:Oligospermia / Q000235:genetics*; D011944:Receptors, Androgen / Q000235:genetics*; D012729:Sex Chromosome Aberrations; D058533:Sex Chromosome Disorders of Sex Development; D013076:Sperm Count; D013091:Spermatogenesis / Q000235:genetics
chemical_list: C410798:AR protein, human; D011944:Receptors, Androgen