title: Production of the Phanerochaete flavido-alba laccase in Aspergillus niger for synthetic dyes decolorization and biotransformation.
abstract: We investigated the expression of Phanerochaete flavido-alba laccase gene in Aspergillus niger and the physical and biochemical properties of the recombinant enzyme (rLac-LPFA) in order to test it for synthetic dye biotransformation. A. niger was able to produce high levels of active recombinant enzyme (30 mgL(-1)), whose identity was further confirmed by immunodetection using Western blot analysis and N-terminal sequencing. Interestingly, rLac-LPFA exhibited an improved stability at pH (2-9) and organic solvents tested. Furthermore, the percentage of decoloration and biotransformation of synthetic textile dyes, Remazol Brilliant Blue R (RBBR) and Acid Red 299 (NY1), was higher than for the native enzyme. Its high production, simple purification, high activity, stability and ability to transform textile dyes make rLac-LPFA a good candidate for industrial applications.
authors.affiliation: Department of Microbiology, Faculty of Pharmacy, University of Granada, Campus Cartuja, 18071, Granada, Spain.
authors.forename: Lamiae
authors.initials: L
authors.lastname: Benghazi
authors.forename: Eric
authors.initials: E
authors.lastname: Record
authors.forename: Antonio
authors.initials: A
authors.lastname: Suárez
authors.forename: José A
authors.initials: JA
authors.lastname: Gomez-Vidal
authors.forename: José
authors.initials: J
authors.lastname: Martínez
authors.forename: Teresa
authors.initials: T
authors.lastname: de la Rubia
mesh_terms: D000880:Anthraquinones / Q000378:metabolism*; D001234:Aspergillus niger / Q000235:genetics / Q000378:metabolism*; D001711:Biotransformation; D015153:Blotting, Western; D004396:Coloring Agents / Q000378:metabolism*; D004795:Enzyme Stability; D015870:Gene Expression; D006863:Hydrogen-Ion Concentration; D042845:Laccase / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D020075:Phanerochaete / Q000201:enzymology* / Q000235:genetics; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D012235:Rhodamines / Q000378:metabolism*; D020539:Sequence Analysis, Protein; D012997:Solvents
chemical_list: D000880:Anthraquinones; D004396:Coloring Agents; D011994:Recombinant Proteins; D012235:Rhodamines; D012997:Solvents; C022027:lissamine rhodamine B; D042845:Laccase; C024758:Remazol Brilliant Blue R