title: High performance liquid chromatographic determination of residual monomer released from heat-cured acrylic resin. An in vivo study.
abstract: OBJECTIVE
  Heat-polymerized acrylic resins are used in dentistry for complete denture fabrication. Despite the polymerization method, conversion of monomer into polymer is often incomplete with free or unreacted residual monomer remaining in the polymerized resin. The aim of this study was to determine the amount of residual monomeric methyl methacrylate (MMA) leaching in the saliva of patients wearing complete dentures in their postinsertion period.
  
  
  METHODS
  Thirty edentulous participants as first-time complete denture wearers (age 60 to 65 years) were selected. All the prostheses were fabricated using a similar standard technique with a heat-cured acrylic resin denture base material. Saliva samples were collected at time intervals of 1 hour, 1 day, and 3 days postdenture insertion. Participants were asked to discharge saliva every 30 seconds into a pre-weighed screw-capped container for a 5-minute period. MMA levels were measured using high performance liquid chromatography. Data were analyzed by ANOVA and Tukey-HSD.
  
  
  RESULTS
  The maximum concentration of monomer released into saliva peaked 1 day after insertion of the complete dentures. The mean (SD) MMA content was 0.04 ± 0.01 (μg/ml) 1 hour after insertion, and 0.3 ± 0.09 (μg/ml), and 0.05 ± 0.01 (μg/ml) on the first and third days postinsertion, respectively.
  
  
  CONCLUSIONS
  Although the released monomeric MMA was not at toxic levels, it could potentially sensitize complete denture patients or elicit an allergic reaction. The risk of the residual material as a primary irritant for a sensitizing reaction could be minimized by immersion of the denture in water for 24 hours before insertion.
authors.affiliation: Department of Prosthodontics, Faculty of Dental Sciences, King George's Medical University UP, Lucknow, India. raghuwards@rediffmail.com
authors.forename: Raghuwar D
authors.initials: RD
authors.lastname: Singh
authors.forename: Rupali
authors.initials: R
authors.lastname: Gautam
authors.forename: Ramashanker
authors.initials: R
authors.lastname: Siddhartha
authors.forename: Balendra P
authors.initials: BP
authors.lastname: Singh
authors.forename: Pooran
authors.initials: P
authors.lastname: Chand
authors.forename: Vinod P
authors.initials: VP
authors.lastname: Sharma
authors.forename: Sunit K
authors.initials: SK
authors.lastname: Jurel
mesh_terms: D000180:Acrylic Resins / Q000032:analysis* / Q000737:chemistry; D000368:Aged; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003764:Dental Materials / Q000032:analysis* / Q000737:chemistry; D003819:Denture Bases; D003779:Denture Design; D003824:Denture, Complete; D004058:Diffusion; D005500:Follow-Up Studies; D006358:Hot Temperature; D006801:Humans; D020366:Methylmethacrylate / Q000032:analysis* / Q000737:chemistry; D008875:Middle Aged; D009066:Mouth, Edentulous / Q000534:rehabilitation; D058105:Polymerization; D011312:Pressure; D012463:Saliva / Q000737:chemistry*
chemical_list: D000180:Acrylic Resins; D003764:Dental Materials; D020366:Methylmethacrylate
keywords: Complete denture; heat-cured acrylic resin; high performance liquid chromatography; residual monomer methyl methacrlylate; saliva