title: High-throughput, high-resolution Echelle deep-UV Raman spectrometer.
abstract: We constructed an ultrahigh-throughput, high-resolution ultraviolet (UV) Raman spectrograph that utilizes a high-efficiency filter-stage monochromator and a high-dispersion Echelle spectrograph. The spectrograph utilizes a total of six mirrors and two gratings, with an overall efficiency at 229 nm of ~18%. The limiting resolution of our spectrometer is 0.6 cm⁻¹ full width half-maximum (FWHM), as measured for 229 nm Rayleigh scattering. Use of a 1 mm-wide entrance slit gives rise to an approximately 10 cm⁻¹ FWHM resolution at 229 nm. The ultrahigh spectrograph throughput enables ultrahigh signal-to-noise ratio, deep UV Raman spectra that allow us to monitor <1% changes in peptide bond composition. The throughput is measured to be 35-fold greater than conventional deep UV Raman spectrometers.
authors.affiliation: Department of Chemistry, University of Pittsburgh, Pittsburgh, PA 15260, USA.
authors.forename: Sergei V
authors.initials: SV
authors.lastname: Bykov
authors.forename: Bhavya
authors.initials: B
authors.lastname: Sharma
authors.forename: Sanford A
authors.initials: SA
authors.lastname: Asher
mesh_terms: D004867:Equipment Design; D057166:High-Throughput Screening Assays / Q000295:instrumentation* / Q000379:methods; D010455:Peptides / Q000032:analysis*; D011487:Protein Conformation; D013056:Spectrophotometry, Ultraviolet / Q000295:instrumentation* / Q000379:methods; D013059:Spectrum Analysis, Raman / Q000295:instrumentation* / Q000379:methods
chemical_list: D010455:Peptides