title: Bowman-Birk inhibitor-like protein is secreted by sprouted pea seeds in response to induced colonization by enteropathogenic Escherichia coli.
abstract: The interaction between the clinical isolate of enteropathogenic Escherichia coli (EPEC) SBANU8 and pea sprouts was compared with avirulent K 12. E. coli. This was carried out by repeated co-incubation with pea sprouts for 5 days, and the protein profile of the culture supernatant was analyzed by single and two-dimensional electrophoresis. Mass spectrometry analysis led to the identification of two serine protease inhibitors including a Bowman-Birk-type protein secreted by pea sprouts in response to clinical isolate. Expression of the E. coli intimin gene involved in animal host colonization and virulence was studied by reverse transcription polymerase chain reaction. Expression of this gene was high in SBANU8 when co-incubated with pea sprouts. The present study gives baseline data on the molecular level interactions of EPEC and pea sprouts, which are needed to design the outbreak control strategies.
authors.affiliation: 1 School of Bio Sciences and Technology, VIT University , Vellore, India .
authors.forename: Ravi
authors.initials: R
authors.lastname: Anuradha
authors.forename: Muthuraj
authors.initials: M
authors.lastname: Raveendran
authors.forename: Subramanian
authors.initials: S
authors.lastname: Babu
mesh_terms: D018829:Adhesins, Bacterial / Q000235:genetics / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D054308:Enteropathogenic Escherichia coli / Q000235:genetics / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism; D005506:Food Contamination / Q000517:prevention & control; D005516:Food Microbiology; D015964:Gene Expression Regulation, Bacterial; D054884:Host-Pathogen Interactions; D018532:Pisum sativum / Q000737:chemistry / Q000382:microbiology*; D010940:Plant Proteins / Q000378:metabolism*; D012329:RNA, Bacterial / Q000235:genetics; D012639:Seeds / Q000737:chemistry / Q000382:microbiology; D014361:Trypsin Inhibitors / Q000378:metabolism*
chemical_list: D018829:Adhesins, Bacterial; D029968:Escherichia coli Proteins; D010940:Plant Proteins; D012329:RNA, Bacterial; D014361:Trypsin Inhibitors; C073819:eaeA protein, E coli