title: Factors influencing the performance of G-quadruplex DNAzyme-based sensors.
abstract: G-quadruplex DNAzymes are peroxidase-like complexes formed by nucleic acid G-quadruplexes and hemin. Compared with natural enzymes, G-quadruplex DNAzyme offers many advantages, thus making it a promising tool in the design of biosensors and chemical sensors. Many biosensors and chemical sensors based on G-quadruplex DNAzymes have been reported. A number of factors may affect the performance of G-quadruplex DNAzyme-based sensors. Here we focus on some aspects to be taken into account when designing a G-quadruplex DNAzyme-based sensor. These include the G-quadruplex-forming G-rich sequence, solution components, the reaction substrate, and enrichment strategy for G-quadruplex DNAzyme. We also provide an outlook for further research on G-quadruplex DNAzyme-based sensors.
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology, Nankai University, Tianjin 300071, PR China; Key Laboratory of Functional Polymer Materials, Ministry of Education, Nankai University, Tianjin 300071, PR China. Electronic address: kongdem@nankai.edu.cn.
authors.forename: De-Ming
authors.initials: DM
authors.lastname: Kong
mesh_terms: D001483:Base Sequence; D055162:Biocatalysis; D015374:Biosensing Techniques; D021881:DNA, Catalytic / Q000737:chemistry*; D005090:Exodeoxyribonucleases / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry; D054856:G-Quadruplexes; D020862:GC Rich Sequence; D006801:Humans; D021141:Nucleic Acid Amplification Techniques; D012996:Solutions
chemical_list: D021881:DNA, Catalytic; D005456:Fluorescent Dyes; D012996:Solutions; D005090:Exodeoxyribonucleases; C020133:exodeoxyribonuclease III
keywords: DNAzyme; Enrichment strategies; G-quadruplex; G-rich sequence; Influencing factors; Sensor