title: An affinity capture involved enzymatic assay for thrombin by using peptide aptamers as affinity ligands on magnetic beads.
abstract: Here we present a sensitive and specific assay for thrombin through the affinity capture of thrombin with mRNA display generated peptide aptamers on magnetic beads and the subsequent thrombin-involved enzymatic reaction. Thrombin at 50 fM can be detected.
authors.affiliation: Research Center for Environmental Science and Engineering, Shanxi University, Taiyuan 030006, China. chemzhaoq@hotmail.com
authors.forename: Qiang
authors.initials: Q
authors.lastname: Zhao
authors.forename: Jie
authors.initials: J
authors.lastname: Gao
mesh_terms: D052158:Aptamers, Peptide / Q000737:chemistry*; D015374:Biosensing Techniques; D057075:Enzyme Assays; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D008024:Ligands; D008280:Magnetics; D013917:Thrombin / Q000032:analysis*
chemical_list: D052158:Aptamers, Peptide; D005456:Fluorescent Dyes; D008024:Ligands; D013917:Thrombin