title: Presence of different genotypes of Helicobacter pylori in patients with chronic tonsillitis and sleep apnoea syndrome.
abstract: Helicobacter pylori, a well-known gastric pathogen, has been detected in the oral cavity and oropharynx in tonsillar tissue. In our study, the presence of H. pylori in the tonsillar tissue of patients with chronic tonsillitis and sleep apnoea syndrome (SAS) was investigated. The aim was to detect and genotype H. pylori for a collection of data supporting the possible role of H. pylori in the aetiology of chronic tonsillitis and SAS. Helicobacter pylori was detected by real-time polymerase chain reaction (rt-PCR). 89 patients, 60 with a diagnosis of chronic tonsillitis and 29 with SAS, were tested. In the chronic tonsillitis group, Helicobacter was detected in 48 (80 %) specimens, cagA gene was detected in 12 samples (25 %) and 12 samples were negative. In SAS group, Helicobacter was found in 24 samples (82.76 %), cagA gene was detected in 5 (20.83 %) and 5 samples (17.24 %) were negative. Helicobacter pylori-specific immunoglobulins were tested by ELISA in the serum of 57 patients only with 41 (71.93 %) showing positive. Our results on H. pylori DNA detection and H. pylori seropositivity show 26.32 % discrepancy, slightly in favour of rt-PCR (15.79 % compared to 10.53 %). The H. pylori presence in tonsillar tissue does not depend on the type of oropharyngeal disease (p = 0.756). This study shows that oropharynx constitutes an extragastric reservoir of H. pylori infection which could serve as an aetiopathogenetic factor for chronic tonsillitis and tonsillar hyperplasia by SAS. No conclusion has yet been drawn about the mechanism of the process.
authors.affiliation: Department of Othorinolaryngology and Head and Neck Surgery, 1st Faculty of Medicine, University Hospital Motol, Charles University, Prague, Czech Republic, anave@volny.cz.
authors.forename: Eva
authors.initials: E
authors.lastname: Nártová
authors.forename: Jaroslav
authors.initials: J
authors.lastname: Kraus
authors.forename: Emil
authors.initials: E
authors.lastname: Pavlík
authors.forename: Petr
authors.initials: P
authors.lastname: Lukeš
authors.forename: Rami
authors.initials: R
authors.lastname: Katra
authors.forename: Jan
authors.initials: J
authors.lastname: Plzák
authors.forename: Libuše
authors.initials: L
authors.lastname: Kolářová
authors.forename: Ivan
authors.initials: I
authors.lastname: Sterzl
authors.forename: Jan
authors.initials: J
authors.lastname: Betka
authors.forename: Jaromír
authors.initials: J
authors.lastname: Astl
mesh_terms: D000328:Adult; D000368:Aged; D000907:Antibodies, Bacterial / Q000276:immunology*; D002908:Chronic Disease; D015331:Cohort Studies; D004269:DNA, Bacterial / Q000235:genetics*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005838:Genotype; D016481:Helicobacter Infections / Q000276:immunology / Q000382:microbiology*; D016480:Helicobacter pylori / Q000235:genetics* / Q000276:immunology; D006801:Humans; D008297:Male; D008875:Middle Aged; D014066:Palatine Tonsil / Q000382:microbiology*; D060888:Real-Time Polymerase Chain Reaction; D012891:Sleep Apnea Syndromes / Q000276:immunology / Q000382:microbiology* / Q000601:surgery; D014068:Tonsillectomy; D014069:Tonsillitis / Q000276:immunology / Q000382:microbiology* / Q000601:surgery; D055815:Young Adult
chemical_list: D000907:Antibodies, Bacterial; D004269:DNA, Bacterial