title: [Activation of JAK/STAT signaling pathway in S2 Drosophila melanogaster cell culture].
abstract: JAK/STAT signaling pathway plays a critical role in different ontogenesis processes of higher eukaryotes. Fruit fly drosophila is a handy model system used to study this pathway since major components of the pathway are represented by unique factors. This article describes the usage of Drosophila melanogaster S2 cells in studies of the pathway's target genes activation. We showed that S2 cells contain plenty of STAT protein which migrates into nucleus under cells treatment with pervanadate. Then we demonstrated that under pervanadate action STAT protein along with other transcription factors is recruited onto regulatory sequences of target genes and activates their transcription.
authors.forename: A V
authors.initials: AV
authors.lastname: Shaposhnikov
authors.forename: A S
authors.initials: AS
authors.lastname: Kryndushkin
authors.forename: Iu V
authors.initials: IuV
authors.lastname: Nikolenko
authors.forename: V V
authors.initials: VV
authors.lastname: Panov
authors.forename: E N
authors.initials: EN
authors.lastname: Nabirochkina
authors.forename: L A
authors.initials: LA
authors.lastname: Lebedeva
authors.forename: Iu V
authors.initials: IuV
authors.lastname: Shidlovskiĭ
mesh_terms: D021581:Active Transport, Cell Nucleus / Q000187:drug effects / Q000502:physiology; D000818:Animals; D002460:Cell Line; D002467:Cell Nucleus / Q000235:genetics / Q000378:metabolism*; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D004331:Drosophila melanogaster; D004791:Enzyme Inhibitors / Q000494:pharmacology; D053612:Janus Kinases / Q000235:genetics / Q000378:metabolism*; D020218:Response Elements; D050791:STAT Transcription Factors / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic / Q000187:drug effects / Q000502:physiology*; D014638:Vanadates / Q000494:pharmacology
chemical_list: D029721:Drosophila Proteins; D004791:Enzyme Inhibitors; D050791:STAT Transcription Factors; D014157:Transcription Factors; C076715:pervanadate; D014638:Vanadates; D053612:Janus Kinases; C085845:hop protein, Drosophila