title: MicroRNA profiling in the serums of SCA3/MJD patients.
abstract: Spinocerebellar ataxia type 3/Machado-Joseph disease (SCA3/MJD) is the most common type of spinocerebellar ataxia in China. However, the pathogenesis of SCA3/MJD is still unknown. MicroRNAs (miRNAs) have been repeatedly demonstrated to exist in human peripheral serum in a bio-stable form and have been shown to be useful biomarkers for other neurodegenerative disorders. However, no study of SCA3/MJD patients has assessed specific changes in regulatory miRNAs. Therefore, we systematically used the miRCURYTM LNA Array, followed by quantitative real-time polymerase chain reaction validation, to access miRNA expression levels in the serums from SCA3/MJD patients. Our results show that miR-25, miR-125b, miR-29a, and miR-34b could be potential biomarkers for SCA3/MJD and could be used to further investigate the pathogenesis of SCA3/MJD and drug development.
authors.affiliation: 1Department of Neurology, Xiangya Hospital, Central South University , Changsha, Hunan , China.
authors.forename: Yuting
authors.initials: Y
authors.lastname: Shi
authors.forename: Fengzhen
authors.initials: F
authors.lastname: Huang
authors.forename: Beisha
authors.initials: B
authors.lastname: Tang
authors.forename: Jiada
authors.initials: J
authors.lastname: Li
authors.forename: Junling
authors.initials: J
authors.lastname: Wang
authors.forename: Lu
authors.initials: L
authors.lastname: Shen
authors.forename: Kun
authors.initials: K
authors.lastname: Xia
authors.forename: Hong
authors.initials: H
authors.lastname: Jiang
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D018450:Disease Progression; D006801:Humans; D017827:Machado-Joseph Disease / Q000097:blood* / Q000235:genetics*; D035683:MicroRNAs / Q000097:blood*; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis; D055815:Young Adult
chemical_list: D015415:Biomarkers; D035683:MicroRNAs