title: Acinetobacter phage genome is similar to Sphinx 2.36, the circular DNA copurified with TSE infected particles.
abstract: While analyzing plasmids of Acinetobacter sp. DS002 we have detected a circular DNA molecule pTS236, which upon further investigation is identified as the genome of a phage. The phage genome has shown sequence similarity to the recently discovered Sphinx 2.36 DNA sequence co-purified with the Transmissible Spongiform Encephalopathy (TSE) particles isolated from infected brain samples collected from diverse geographical regions. As in Sphinx 2.36, the phage genome also codes for three proteins. One of them codes for RepA and is shown to be involved in replication of pTS236 through rolling circle (RC) mode. The other two translationally coupled ORFs, orf106 and orf96, code for coat proteins of the phage. Although an orf96 homologue was not previously reported in Sphinx 2.36, a closer examination of DNA sequence of Sphinx 2.36 revealed its presence downstream of orf106 homologue. TEM images and infection assays revealed existence of phage AbDs1 in Acinetobacter sp. DS002.
authors.affiliation: Department of Animal Sciences, School of Life Sciences, University of Hyderabad, Hyderabad - 500 046, India.
authors.forename: Toshisangba
authors.initials: T
authors.lastname: Longkumer
authors.forename: Swetha
authors.initials: S
authors.lastname: Kamireddy
authors.forename: Venkateswar Reddy
authors.initials: VR
authors.lastname: Muthyala
authors.forename: Shaikh
authors.initials: S
authors.lastname: Akbarpasha
authors.forename: Gopi Krishna
authors.initials: GK
authors.lastname: Pitchika
authors.forename: Gopinath
authors.initials: G
authors.lastname: Kodetham
authors.forename: Murali
authors.initials: M
authors.lastname: Ayaluru
authors.forename: Dayananda
authors.initials: D
authors.lastname: Siddavattam
mesh_terms: D000150:Acinetobacter / Q000821:virology*; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D001435:Bacteriophages / Q000235:genetics* / Q000648:ultrastructure; D001483:Base Sequence; D018387:Codon, Initiator; D004261:DNA Replication; D004270:DNA, Circular; D016679:Genome, Viral; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D010957:Plasmids / Q000235:genetics; D017096:Prion Diseases / Q000378:metabolism; D011485:Protein Binding; D018741:Replication Origin; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
chemical_list: D001426:Bacterial Proteins; D018387:Codon, Initiator; D004270:DNA, Circular