title: The regulation of Toll-like receptor 2 by miR-143 suppresses the invasion and migration of a subset of human colorectal carcinoma cells.
abstract: BACKGROUND
  The Toll-like receptor 2 (TLR2)-driven tissue response may promote neoangiogenesis and tumour growth by mechanisms that are poorly understood.
  
  
  METHODS
  We investigated the expression levels of TLR2 and associated-miRNAs in colorectal carcinoma (CRC) tissues and cell lines using real-time PCR, northern blotting and western blotting. Survival curver was generated by Log-Rank test and the role of TLR2 signalling in tumour invasion and migration was determined by transwell analysis kits.
  
  
  RESULTS
  We observed that the tissues from CRC patients express relatively high levels of TLR2. Targeting TLR2 markedly reduces the invasion and migration of CRC cells. We also found that miR-143, a putative tumour suppressor that is down-regulated in CRC tissues, reduces the invasion and migration of CRC cells primarily via TLR2. Utilising a xenograft mouse model, we demonstrated that re-expression of miR-143 inhibits CRC cell colonisation in vivo.
  
  
  CONCLUSIONS
  miR-143 blocks the TLR2 signalling pathway in human CRC cells. This knowledge may pave the way for new clinical applications utilising miR-143 mimics in the treatment of patients with CRC.
authors.affiliation: Department of Clinical Laboratory, Shanghai Third People's Hospital, School of medicine, Shanghai Jiao Tong University, Shanghai 200019, PR China. sxguohaiyan@126.com
authors.forename: Haiyan
authors.initials: H
authors.lastname: Guo
authors.forename: Ying
authors.initials: Y
authors.lastname: Chen
authors.forename: Xiaobo
authors.initials: X
authors.lastname: Hu
authors.forename: Guanxiang
authors.initials: G
authors.lastname: Qian
authors.forename: Shengfang
authors.initials: S
authors.lastname: Ge
authors.forename: Jianjun
authors.initials: J
authors.lastname: Zhang
mesh_terms: D000818:Animals; D020029:Base Pairing; D001483:Base Sequence; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics*; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology*; D004195:Disease Models, Animal; D015870:Gene Expression; D006801:Humans; D051379:Mice; D035683:MicroRNAs / Q000737:chemistry / Q000235:genetics*; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D051195:Toll-Like Receptor 2 / Q000235:genetics* / Q000378:metabolism; D014183:Transplantation, Heterologous
chemical_list: C542359:MIRN143 microRNA, human; D035683:MicroRNAs; D051195:Toll-Like Receptor 2