title: A new method for the detection of neutralizing antibodies against mumps virus.
abstract: Neutralization test is the most reliable method of evaluating immunity against viral diseases but there is no standard procedure for mumps virus, with tests differing in the infectivity of the challenge virus, 50% plaque reduction or complete inhibition of cytopathic effects (CPE), and usage of complement. A reliable, easy, and simple neutralization test for mumps virus was developed in this study. A recombinant mumps virus expressing GFP was generated as a challenge virus. Complement was added to the neutralizing mixture at 1∶200 when stocked serum samples were used. Neutralizing antibody titers were expressed as the reciprocal of the highest dilution that did not exceed two-fold of FU values (GFP expression) of the cell control wells. A total of 1,452 serum samples were assayed by inhibition of GFP expression in comparison with those examined by conventional 100% inhibition of CPE. 1,367 (94.1%) showed similar neutralizing antibody titers when examined by both methods. The GFP expression inhibition assay, using a recombinant mumps virus expressing GFP, is a simple and time- saving method.
authors.affiliation: Department of Pediatrics, Hiroshima Prefectural Hospital, Minami-ku, Hiroshima City, Hiroshima, Japan.
authors.forename: Keita
authors.initials: K
authors.lastname: Matsubara
authors.forename: Motoko
authors.initials: M
authors.lastname: Fujino
authors.forename: Kaoru
authors.initials: K
authors.lastname: Takeuchi
authors.forename: Satoshi
authors.initials: S
authors.lastname: Iwata
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Nakayama
mesh_terms: D000328:Adult; D000818:Animals; D057134:Antibodies, Neutralizing / Q000097:blood / Q000276:immunology*; D000914:Antibodies, Viral / Q000097:blood / Q000276:immunology*; D002460:Cell Line; D002522:Chlorocebus aethiops; D003165:Complement System Proteins / Q000276:immunology; D003588:Cytopathogenic Effect, Viral; D015870:Gene Expression; D023061:Gene Order; D017930:Genes, Reporter; D005822:Genetic Vectors / Q000235:genetics / Q000276:immunology; D006801:Humans; D008875:Middle Aged; D009107:Mumps / Q000175:diagnosis* / Q000276:immunology*; D009109:Mumps virus / Q000235:genetics / Q000276:immunology*; D009500:Neutralization Tests / Q000379:methods*; D013696:Temperature; D014709:Vero Cells; D010948:Viral Plaque Assay; D055815:Young Adult
chemical_list: D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D003165:Complement System Proteins