title: The effect of high-calorie meal consumption on oxidative stress and endothelial dysfunction in healthy male adults.
abstract: Several authors have reported the association of postprandial hypertriglyceridemia with oxidative stress, systemic inflammation and endothelial dysfunction. Our aim was to investigate the effect of high-calorie meal on blood markers of oxidative stress and endothelial dysfunction and the association of APOA5 -1131T/C and -250G/A hepatic lipase (HL) polymorphisms with postprandial triglyceride response. This study included 102 healthy male volunteers. All participants consumed a high-calorie meal (823 calories, 50 g fat, 28 g protein, 60 g carbohydrates). Total cholesterol, triglycerides, HDL-cholesterol, LDL-cholesterol, hsCRP, TAS and ICAM-1 were measured at fasting state and postprandially. APOA5 -1131T/C and -250G/A HL polymorphisms were also determined. Postprandial triglycerides were significantly increased (1.4 (1.1-2.1) vs. 2.4 (1.9-3.3) mmol/l, P<0.001). Average triglyceride increase was 1.0+/-0.7 mmol/l (65 %). Concentration of triglycerides, HDL-cholesterol, LDL-cholesterol, TAS and ICAM-1 differed significantly between the fasting state and postprandial measurements (P<0.001). However, those differences were within the limits of analytical imprecision. Other parameters did not change 3 h after the meal. Triglycerides response did not differ respective to the APOA5 and HL polymorphisms. Family history of hypertension and acute myocardial infarction were associated with higher postprandial triglyceride concentrations. Postprandial hypertriglyceridemia is not associated with increased concentrations of hsCRP, TAS and ICAM-1. Furthermore, APOA5 -1131T/C and -250G/A HL polymorphisms are not associated with different postprandial triglyceride response.
authors.affiliation: Medical biochemistry laboratory, Policlinic Bonifarm, Zagreb, Croatia. sanjakackov@hotmail.com.
authors.forename: S
authors.initials: S
authors.lastname: Kackov
authors.forename: A-M
authors.initials: AM
authors.lastname: Simundic
authors.forename: N
authors.initials: N
authors.lastname: Nikolac
authors.forename: I
authors.initials: I
authors.lastname: Celap
authors.forename: L
authors.initials: L
authors.lastname: Dukic
authors.forename: D
authors.initials: D
authors.lastname: Ruzic
authors.forename: M
authors.initials: M
authors.lastname: Bilusic
mesh_terms: D000328:Adult; D000368:Aged; D000072040:Apolipoprotein A-V; D001054:Apolipoproteins A / Q000235:genetics*; D004041:Dietary Fats / Q000378:metabolism*; D004730:Endothelium, Vascular / Q000503:physiopathology*; D002149:Energy Intake; D006801:Humans; D015228:Hypertriglyceridemia / Q000503:physiopathology*; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D019518:Postprandial Period; D012016:Reference Values; D014280:Triglycerides / Q000097:blood*
chemical_list: C437704:APOA5 protein, human; D000072040:Apolipoprotein A-V; D001054:Apolipoproteins A; D004041:Dietary Fats; D014280:Triglycerides