title: Mapping histologic patchiness of celiac disease by push enteroscopy.
abstract: BACKGROUND
  Despite great improvements in serologic testing, duodenal biopsies are still required to diagnose the majority of celiac disease (CD) cases. Nevertheless, the histologic pattern of CD is often patchy, leading to the risk of missing the diagnosis.
  
  
  OBJECTIVE
  To evaluate the patchiness of the CD histologic lesions along the small bowel (SB), push enteroscopy has been performed instead of conventional upper GI endoscopy.
  
  
  METHODS
  Prospective, single-center study.
  
  
  METHODS
  Tertiary-care referral center.
  
  
  METHODS
  A total of 41 pediatric patients with suspected CD.
  
  
  METHODS
  Prospective evaluation of bulb, duodenal, and jejunal biopsy specimens in the diagnosis of CD.
  
  
  METHODS
  Pattern of lesion distribution along the SB (from bulb up to 60 cm beyond the ligament of Treitz) and yield as well accuracy of pediatric CD diagnosis by using push enteroscopy.
  
  
  RESULTS
  There was a homogeneous pattern of histologic damage in 17 patients (41.5%), whereas 24 patients (58.5%) had a lesion pattern of patchiness. The second and fourth duodenal regions were involved in 38 children (92.7%) and 37 children (90.2%), respectively; the bulb was involved in 37 patients (90.2%); both distal and proximal jejunal samples showed histologic lesions in 38 children (92.7%). In 1 patient, without lesions in the bulb and duodenum, CD was diagnosed according to proximal and distal jejunal biopsies only (3B and C, respectively). A significant correlation was found between the degree of villous atrophy and the serum anti-transglutaminase titer.
  
  
  CONCLUSIONS
  Small sample size; academic tertiary-care setting.
  
  
  CONCLUSIONS
  CD histologic lesions often have a discontinuous distribution along the SB, occasionally with an exclusive jejunal involvement. A high degree of villous atrophy correlates with a high anti-transglutaminase titer. When the new ESPGHAN "biopsy-sparing" criteria are not applicable, in case of potential CD, push enteroscopy might be a valuable second-step tool to re-evaluate and identify false "potential" CD hiding exclusive jejunal lesions.
authors.affiliation: Pediatric Gastroenterology and Liver Unit, Department of Pediatrics, Sapienza University of Rome, Rome, Italy.
authors.forename: Francesco
authors.initials: F
authors.lastname: Valitutti
authors.affiliation: Pediatric Gastroenterology and Liver Unit, Department of Pediatrics, Sapienza University of Rome, Rome, Italy.
authors.forename: Giovanni
authors.initials: G
authors.lastname: Di Nardo
authors.affiliation: Pediatric Gastroenterology and Liver Unit, Department of Pediatrics, Sapienza University of Rome, Rome, Italy.
authors.forename: Maria
authors.initials: M
authors.lastname: Barbato
authors.affiliation: Pediatric Gastroenterology and Liver Unit, Department of Pediatrics, Sapienza University of Rome, Rome, Italy.
authors.forename: Marina
authors.initials: M
authors.lastname: Aloi
authors.affiliation: Pediatric Gastroenterology and Liver Unit, Department of Pediatrics, Sapienza University of Rome, Rome, Italy.
authors.forename: Ilaria
authors.initials: I
authors.lastname: Celletti
authors.affiliation: Pediatric Gastroenterology and Liver Unit, Department of Pediatrics, Sapienza University of Rome, Rome, Italy.
authors.forename: Chiara Maria
authors.initials: CM
authors.lastname: Trovato
authors.affiliation: ENEA, Italian National Agency for new Technologies, Energy and Sustainable Economic Development, Rome, Italy.
authors.forename: Maria
authors.initials: M
authors.lastname: Pierdomenico
authors.affiliation: Department of Clinical Science, Sapienza University of Rome, Rome, Italy.
authors.forename: Adriana
authors.initials: A
authors.lastname: Marcheggiano
authors.affiliation: Pediatric Gastroenterology and Liver Unit, Department of Pediatrics, Sapienza University of Rome, Rome, Italy.
authors.forename: Salvatore
authors.initials: S
authors.lastname: Cucchiara
mesh_terms: D000293:Adolescent; D000906:Antibodies / Q000097:blood; D001706:Biopsy; D002446:Celiac Disease / Q000097:blood / Q000473:pathology*; D002648:Child; D002675:Child, Preschool; D004386:Duodenum / Q000473:pathology*; D016099:Endoscopy, Gastrointestinal / Q000379:methods*; D005260:Female; D019204:GTP-Binding Proteins; D006801:Humans; D007223:Infant; D007583:Jejunum / Q000473:pathology*; D008297:Male; D011446:Prospective Studies; D000091345:Protein Glutamine gamma Glutamyltransferase 2; D011503:Transglutaminases / Q000276:immunology
chemical_list: D000906:Antibodies; D000091345:Protein Glutamine gamma Glutamyltransferase 2; D011503:Transglutaminases; D019204:GTP-Binding Proteins
keywords: CD; ESPGHAN; MO; Marsh-Oberhuber; The European Society for Paediatric Gastroenterology Hepatology and Nutrition; celiac disease