title: Conclusive evidence of the reconstituted hexasome proven by native mass spectrometry.
abstract: It has been suggested that the hexasome, in which one of the H2A/H2B dimers is depleted from the canonical nucleosome core particle (NCP), is an essential intermediate during NCP assembly and disassembly, but little structural evidence of this exists. In this study, reconstituted products in a conventional NCP preparation were analyzed by native electrospray ionization mass spectrometry, and it was found that the hexasome, which migrated in a manner almost identical to that of the octasome NCP in native polyacrylamide gel electrophoresis, was produced simultaneously with the octasome NCP. This result might contribute to understanding the assembly and disassembly mechanism of NCPs.
authors.affiliation: Graduate School of Nanobioscience, Yokohama City University , 1-7-29 Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan.
authors.forename: Nanako
authors.initials: N
authors.lastname: Azegami
authors.forename: Kazumi
authors.initials: K
authors.lastname: Saikusa
authors.forename: Yasuto
authors.initials: Y
authors.lastname: Todokoro
authors.forename: Aritaka
authors.initials: A
authors.lastname: Nagadoi
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Kurumizaka
authors.forename: Yoshifumi
authors.initials: Y
authors.lastname: Nishimura
authors.forename: Satoko
authors.initials: S
authors.lastname: Akashi
mesh_terms: D019281:Dimerization; D006657:Histones / Q000737:chemistry* / Q000378:metabolism*; D009707:Nucleosomes / Q000737:chemistry / Q000378:metabolism; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D006657:Histones; D009707:Nucleosomes