title: Vaginal colonization by papG allele II+ Escherichia coli isolates from pregnant and nonpregnant women as predisposing factor to pyelonephritis.
abstract: Vaginal (61) and fecal (61) Escherichia coli isolates from pregnant and nonpregnant women (18-45 years old) were surveyed for papG alleles by PCR technique. papG allele II was the most prevalent among both vaginal (32.7%) and fecal (3.2%) isolates, whereas other alleles were found only among vaginal isolates (1.6% for alleles I and III and 3.2% for alleles II + III). papG(+) pregnant women's isolates did not differ significantly from those of nonpregnant in possession of papG allele II (90% versus 73.3%), whereas both (32.7%) differed significantly (P ≤ 0.05) in comparison with fecal isolates (3.2%). The vast majority of papG allele II(+) vaginal isolates were clustered in group B2 (81.8%) and much less in group D (18.1%). Also, most of them were positive for fimH (100%), papC (100%), iucC (90.9%), and hly (72.7%), and about half of them were positive for sfa/foc (45.4%). In addition, the mean of VFs' gene possession was 3.5 (range from 2 to 5). It can be concluded that vaginal colonization by papG allele II(+) E. coli is possibly one of the predisposing factors of both pregnant and nonpregnant women to pyelonephritis, but its potential may be modified by other factors especially host factors.
authors.affiliation: Department of Biology, College of Science, University of Wasit, Al-Kut, Wasit Province, Iraq. sareaamaseer@yahoo.com
authors.forename: Sareaa Maseer Gatya
authors.initials: SM
authors.lastname: Al-Mayahie
mesh_terms: D018830:Adhesins, Escherichia coli / Q000235:genetics*; D000328:Adult; D002353:Carrier State / Q000382:microbiology*; D015984:Causality; D016009:Chi-Square Distribution; D015331:Cohort Studies; D004926:Escherichia coli / Q000145:classification / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D004927:Escherichia coli Infections / Q000382:microbiology*; D005243:Feces / Q000382:microbiology; D005260:Female; D036781:Fimbriae Proteins / Q000235:genetics*; D006801:Humans; D010802:Phylogeny; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000382:microbiology*; D011704:Pyelonephritis / Q000382:microbiology; D014621:Vagina / Q000382:microbiology*
chemical_list: D018830:Adhesins, Escherichia coli; C092426:PapG protein, E coli; D036781:Fimbriae Proteins