title: Potential use of DNA barcoding for the identification of Salvia based on cpDNA and nrDNA sequences.
abstract: An effective DNA marker for authenticating the genus Salvia was screened using seven DNA regions (rbcL, matK, trnL-F, and psbA-trnH from the chloroplast genome, and ITS, ITS1, and ITS2 from the nuclear genome) and three combinations (rbcL+matK, psbA-trnH+ITS1, and trnL-F+ITS1). The present study collected 232 sequences from 27 Salvia species through DNA sequencing and 77 sequences within the same taxa from the GenBank. The discriminatory capabilities of these regions were evaluated in terms of PCR amplification success, intraspecific and interspecific divergence, DNA barcoding gaps, and identification efficiency via a tree-based method. ITS1 was superior to the other marker for discriminating between species, with an accuracy of 81.48%. The three combinations did not increase species discrimination. Finally, we found that ITS1 is a powerful barcode for identifying Salvia species, especially Salvia miltiorrhiza.
authors.affiliation: College of Life and Basic Science, Sichuan Agricultural University, Yaan 625014, China.
authors.forename: Meng
authors.initials: M
authors.lastname: Wang
authors.forename: Hong-Xia
authors.initials: HX
authors.lastname: Zhao
authors.forename: Long
authors.initials: L
authors.lastname: Wang
authors.forename: Tao
authors.initials: T
authors.lastname: Wang
authors.forename: Rui-Wu
authors.initials: RW
authors.lastname: Yang
authors.forename: Xiao-Li
authors.initials: XL
authors.lastname: Wang
authors.forename: Yong-Hong
authors.initials: YH
authors.lastname: Zhou
authors.forename: Chun-Bang
authors.initials: CB
authors.lastname: Ding
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
mesh_terms: D001482:Base Composition; D058893:DNA Barcoding, Taxonomic; D018742:DNA, Chloroplast / Q000235:genetics*; D021901:DNA, Intergenic / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics*; D017343:Genes, Plant; D056426:Genetic Loci; D005819:Genetic Markers; D014644:Genetic Variation; D054628:Genome, Chloroplast; D008957:Models, Genetic; D027544:Salvia / Q000235:genetics*; D017422:Sequence Analysis, DNA; D018709:Statistics, Nonparametric
chemical_list: D018742:DNA, Chloroplast; D021901:DNA, Intergenic; D018744:DNA, Plant; D004275:DNA, Ribosomal; D005819:Genetic Markers
keywords: BS; CBOL; Consortium for the Barcode of Life; DNA barcoding; ITS; ITS1; K2P; Kimura 2-Parameter; NJ; PCR; Salvia; bootstrap support; chloroplast DNA; cpDNA; internal transcribed spacer; neighbor-joining; nrDNA; nuclear ribosomal DNA; polymerase chain reaction; trnL–F