title: Quantitative electron tomography: the effect of the three-dimensional point spread function.
abstract: The intensity levels in a three-dimensional (3D) reconstruction, obtained by electron tomography, can be influenced by several experimental imperfections. Such artifacts will hamper a quantitative interpretation of the results. In this paper, we will correct for artificial intensity variations by determining the 3D point spread function (PSF) of a tomographic reconstruction based on high angle annular dark field scanning transmission electron microscopy. The large tails of the PSF cause an underestimation of the intensity of smaller particles, which in turn hampers an accurate radius estimate. Here, the error introduced by the PSF is quantified and corrected a posteriori.
authors.affiliation: EMAT, University of Antwerp, Groenenborgerlaan 171, B-2020 Antwerp, Belgium.
authors.forename: Hamed
authors.initials: H
authors.lastname: Heidari
authors.forename: Wouter
authors.initials: W
authors.lastname: Van den Broek
authors.forename: Sara
authors.initials: S
authors.lastname: Bals
mesh_terms: D016477:Artifacts; D055032:Electron Microscope Tomography / Q000379:methods*; D021621:Imaging, Three-Dimensional / Q000379:methods*; D017348:Microscopy, Electron, Scanning Transmission / Q000379:methods
keywords: Artifact correction; Electron tomography; Radius distribution