title: Phospholipase C-γ2 via p38 and ERK1/2 MAP kinase mediates diperoxovanadate-asparagine induced human platelet aggregation and sCD40L release.
abstract: OBJECTIVE
  Redox imbalance either inside platelets or in their immediate surroundings prove detrimental to their physiologic functions during haemostasis. This study was therefore aimed to assess the effect of peroxide radicals on platelet functions and underlying signalling mechanisms using asparagine-conjugated diperoxovanadate (DPV-Asn).
  
  
  METHODS
  Platelet aggregation, ATP secretion, TxB2 release, intra-platelet calcium mobilization, protein tyrosine phosphorylation, GPIIbIIIa activation by PAC1 labelling and sCD40L release (enzyme-linked immunosorbent assay) was monitored using various concentrations of DPV-Asn. Cell viability was assessed by Annexin V labelling, MTT assay, LDH leakage and mitochondrial membrane potential by JC-1.
  
  
  RESULTS
  Platelet aggregation induced by DPV-Asn was chiefly regulated by dense granule secretion, thromboxane A2 (TxA2) generation, intra-platelet [Ca(2+)] influx, GPIIbIIIa activation and sCD40L release, which were significantly reduced in presence of U73122 (PLC inhibitor), aspirin (COX), SB203580 (p38 inhibitor), and PD98059 (ERK inhibitor). This was further corroborated by enhanced tyrosine phosphorylation of numerous platelet proteins including PLC-γ2, which apparently played a central role in transducing peroxide signals to regulate [Ca(2+)] influx and phosphorylation of p38 and ERK1/2 MAP kinase.
  
  
  CONCLUSIONS
  Peroxide radicals critically regulate the thrombo-inflammatory functions of platelets via the PLCγ2-p38-ERK1/2-TxA2 pathway, which closely resembles the clinical scenario of various pathologies like hyperglycemia and atherosclerosis during which oxidative stress disrupts platelet functions.
authors.affiliation: CSIR-Central Drug Research Institute, Lucknow, Uttar Pradesh, India.
authors.forename: Ankita
authors.initials: A
authors.lastname: Misra
authors.forename: Smriti
authors.initials: S
authors.lastname: Srivastava
authors.forename: Seshadri Reddy
authors.initials: SR
authors.lastname: Ankireddy
authors.forename: Nashreen S
authors.initials: NS
authors.lastname: Islam
authors.forename: Tulika
authors.initials: T
authors.lastname: Chandra
authors.forename: Ashutosh
authors.initials: A
authors.lastname: Kumar
authors.forename: Manoj Kumar
authors.initials: MK
authors.lastname: Barthwal
authors.forename: Madhu
authors.initials: M
authors.lastname: Dikshit
mesh_terms: D001216:Asparagine / Q000494:pharmacology*; D023201:CD40 Ligand / Q000378:metabolism*; D006801:Humans; D015151:Immunoblotting; D047468:Immunoprecipitation; D019950:Mitogen-Activated Protein Kinase 1 / Q000378:metabolism*; D048052:Mitogen-Activated Protein Kinase 3 / Q000378:metabolism*; D010545:Peroxides / Q000494:pharmacology*; D051966:Phospholipase C gamma / Q000378:metabolism*; D010974:Platelet Aggregation / Q000187:drug effects; D015398:Signal Transduction / Q000187:drug effects; D014638:Vanadates / Q000494:pharmacology*
chemical_list: D010545:Peroxides; C091767:diperoxovanadate; D023201:CD40 Ligand; D014638:Vanadates; D001216:Asparagine; D019950:Mitogen-Activated Protein Kinase 1; D048052:Mitogen-Activated Protein Kinase 3; D051966:Phospholipase C gamma