title: Effect of hydroxyethyl starch 130/0.4 and 200/0.5 solutions on canine platelet function in vitro.
abstract: OBJECTIVE
  To determine whether dilution of blood samples from healthy dogs with 2 hydroxyethyl starch (HES) solutions, HES 130/0.4 and HES 200/0.5, would result in platelet dysfunction as measured by closure time (Ct) beyond a dilutional effect.
  
  
  METHODS
  Citrated blood samples from 10 healthy dogs with a Ct within reference limits (52 to 86 seconds).
  
  
  METHODS
  Blood samples were diluted 1:9 and 1:3 with 6% HES 130/0.4 and 10% HES 200/0.5 solutions and saline (0.9% NaCl) solution. Dilutions at 1:9 and 1:3 mimicked 10 mL/kg and 30 mL/kg doses, respectively, ignoring in vivo redistribution. Closure time was measured with a platelet function analyzer and compared among dilutions.
  
  
  RESULTS
  A dilutional effect on Ct was evident for the 1:3 dilution, compared with the 1:9 dilution, but only HES 200/0.5 increased the Ct beyond the dilutional effect at the 1:3 dilution, to a median Ct of 125 seconds (interquartile range, 117.5 to 139.5 seconds). No effect of HES or dilution on Ct was identified at the 1:9 dilution.
  
  
  CONCLUSIONS
  1:3 dilution of blood samples from healthy dogs with HES 200/0.5 but not HES 130/0.4 significantly increased Ct beyond the dilutional effect, suggesting that IV administration of HES 200/0.5 in dogs might cause platelet dysfunction.
authors.affiliation: School of Veterinary and Life Sciences, Murdoch University, Australia. dmcbride@rvc.ac.uk
authors.forename: Duana
authors.initials: D
authors.lastname: McBride
authors.forename: Giselle L
authors.initials: GL
authors.lastname: Hosgood
authors.forename: Caroline S
authors.initials: CS
authors.lastname: Mansfield
authors.forename: Lisa
authors.initials: L
authors.lastname: Smart
mesh_terms: D000818:Animals; D001792:Blood Platelets / Q000187:drug effects*; D004285:Dogs / Q000097:blood*; D005260:Female; D006895:Hydroxyethyl Starch Derivatives / Q000737:chemistry* / Q000494:pharmacology*; D008297:Male; D010974:Platelet Aggregation / Q000187:drug effects*
chemical_list: D006895:Hydroxyethyl Starch Derivatives