title: Genome sequence and functional genomic analysis of the oil-degrading bacterium Oleispira antarctica.
abstract: Ubiquitous bacteria from the genus Oleispira drive oil degradation in the largest environment on Earth, the cold and deep sea. Here we report the genome sequence of Oleispira antarctica and show that compared with Alcanivorax borkumensis--the paradigm of mesophilic hydrocarbonoclastic bacteria--O. antarctica has a larger genome that has witnessed massive gene-transfer events. We identify an array of alkane monooxygenases, osmoprotectants, siderophores and micronutrient-scavenging pathways. We also show that at low temperatures, the main protein-folding machine Cpn60 functions as a single heptameric barrel that uses larger proteins as substrates compared with the classical double-barrel structure observed at higher temperatures. With 11 protein crystal structures, we further report the largest set of structures from one psychrotolerant organism. The most common structural feature is an increased content of surface-exposed negatively charged residues compared to their mesophilic counterparts. Our findings are relevant in the context of microbial cold-adaptation mechanisms and the development of strategies for oil-spill mitigation in cold environments.
authors.affiliation: Max-Planck Institute for Molecular Genetics, Berlin-Dahlem D-14195, Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Kube
authors.forename: Tatyana N
authors.initials: TN
authors.lastname: Chernikova
authors.forename: Yamal
authors.initials: Y
authors.lastname: Al-Ramahi
authors.forename: Ana
authors.initials: A
authors.lastname: Beloqui
authors.forename: Nieves
authors.initials: N
authors.lastname: Lopez-Cortez
authors.forename: María-Eugenia
authors.initials: ME
authors.lastname: Guazzaroni
authors.forename: Hermann J
authors.initials: HJ
authors.lastname: Heipieper
authors.forename: Sven
authors.initials: S
authors.lastname: Klages
authors.forename: Oleg R
authors.initials: OR
authors.lastname: Kotsyurbenko
authors.forename: Ines
authors.initials: I
authors.lastname: Langer
authors.forename: Taras Y
authors.initials: TY
authors.lastname: Nechitaylo
authors.forename: Heinrich
authors.initials: H
authors.lastname: Lünsdorf
authors.forename: Marisol
authors.initials: M
authors.lastname: Fernández
authors.forename: Silvia
authors.initials: S
authors.lastname: Juárez
authors.forename: Sergio
authors.initials: S
authors.lastname: Ciordia
authors.forename: Alexander
authors.initials: A
authors.lastname: Singer
authors.forename: Olga
authors.initials: O
authors.lastname: Kagan
authors.forename: Olga
authors.initials: O
authors.lastname: Egorova
authors.forename: Pierre Alain
authors.initials: PA
authors.lastname: Petit
authors.forename: Peter
authors.initials: P
authors.lastname: Stogios
authors.forename: Youngchang
authors.initials: Y
authors.lastname: Kim
authors.forename: Anatoli
authors.initials: A
authors.lastname: Tchigvintsev
authors.forename: Robert
authors.initials: R
authors.lastname: Flick
authors.forename: Renata
authors.initials: R
authors.lastname: Denaro
authors.forename: Maria
authors.initials: M
authors.lastname: Genovese
authors.forename: Juan P
authors.initials: JP
authors.lastname: Albar
authors.forename: Oleg N
authors.initials: ON
authors.lastname: Reva
authors.forename: Montserrat
authors.initials: M
authors.lastname: Martínez-Gomariz
authors.forename: Hai
authors.initials: H
authors.lastname: Tran
authors.forename: Manuel
authors.initials: M
authors.lastname: Ferrer
authors.forename: Alexei
authors.initials: A
authors.lastname: Savchenko
authors.forename: Alexander F
authors.initials: AF
authors.lastname: Yakunin
authors.forename: Michail M
authors.initials: MM
authors.lastname: Yakimov
authors.forename: Olga V
authors.initials: OV
authors.lastname: Golyshina
authors.forename: Richard
authors.initials: R
authors.lastname: Reinhardt
authors.forename: Peter N
authors.initials: PN
authors.lastname: Golyshin
mesh_terms: D000222:Adaptation, Physiological / Q000235:genetics*; D053521:Alcanivoraceae / Q000235:genetics / Q000378:metabolism; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics; D001673:Biodegradation, Environmental; D002874:Chromosome Mapping; D003080:Cold Temperature; D020563:Gammaproteobacteria / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D022761:Gene Transfer, Horizontal; D059646:Genome Size; D016680:Genome, Bacterial; D007219:Industrial Oils; D018832:Molecular Chaperones / Q000737:chemistry* / Q000235:genetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D017510:Protein Folding; D054712:Salinity; D017422:Sequence Analysis, DNA
chemical_list: D001426:Bacterial Proteins; D018832:Molecular Chaperones