title: Detection of unusual G6 rotavirus strains in Italian children with diarrhoea during the 2011 surveillance season.
abstract: Two rare G6 rotavirus A (RVA) strains, designated as RVA/human-wt/ITA/CEC06/2011/G6P[6] and RVA/human-wt/ITA/PG05/2011/G6P[9], were identified in stool specimens from children hospitalized in Central Italy. After PCR genotyping, the samples CEC06 and PG05 gave G-UD-P[6] and G-UD-P[9] genotypes, respectively. To determine the G-type and to characterize further the two strains, sequencing of 8 of the 11 genomic segments was performed. CEC06 and PG05 strains were found to possess unusual genotype constellations: G6-P[6]-I2-A2-N2-T2-E2-H2 and G6-P[9]-I2-A3-N2-T3-E3-H3, respectively. This study reports the first detection of rare G6P[6] and G6P[9] RVA strains in peninsular Italy. Phylogenetic analysis of VP4 (VP8*), VP7, VP6, and NSP1-5 showed no evidence of zoonosis or inter-species reassortment, revealing for both strains constellations previously associated to human cases.
authors.affiliation: National Center for Immunobiologicals Research and Evaluation, Istituto Superiore di Sanità, Rome, Italy.
authors.forename: Giovanni
authors.initials: G
authors.lastname: Ianiro
authors.forename: Roberto
authors.initials: R
authors.lastname: Delogu
authors.forename: Barbara
authors.initials: B
authors.lastname: Camilloni
authors.forename: Chiara
authors.initials: C
authors.lastname: Lorini
authors.forename: Franco M
authors.initials: FM
authors.lastname: Ruggeri
authors.forename: Lucia
authors.initials: L
authors.lastname: Fiore
mesh_terms: D016000:Cluster Analysis; D005243:Feces / Q000821:virology; D005838:Genotype; D006801:Humans; D007223:Infant; D007558:Italy / Q000453:epidemiology; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000235:genetics; D012401:Rotavirus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D012400:Rotavirus Infections / Q000453:epidemiology* / Q000821:virology*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014764:Viral Proteins / Q000235:genetics
chemical_list: D012367:RNA, Viral; D014764:Viral Proteins
keywords: G6P[6]; G6P[9]; HBGA; Italy; group A rotavirus; sequence