title: Biologically active peptides obtained by enzymatic hydrolysis of Adzuki bean seeds.
abstract: This study investigated the antioxidant and antihypertensive activities of peptides obtained from protein fractions of Adzuki bean seeds. Peptides were obtained by the use of hydrolytic enzymes in vitro under gastrointestinal conditions. A determination was made of the activity of the peptide inhibitors of the angiotensin I converting enzyme (ACE), and the antiradical and ion chelating activity of peptides from different protein fractions. The highest peptide levels after the absorption process (<7 kDa) were noted in the albumin fraction (50.69 μg/ml). Furthermore, it was found that peptides from the prolamin fraction were characterised by the highest antiradical activity and ACE inhibitory activity (IC50=0.17 mg/ml). Peptides obtained from the globulin fraction showed the highest ability to chelate iron ions, and peptides from the glutelin fraction were characterised as being the most effective in the chelation of copper ions.
authors.affiliation: Department of Biochemistry and Food Chemistry, University of Life Sciences, Skromna Str. 8, 20-704 Lublin, Poland. agatadurak@gmail.com
authors.forename: Agata
authors.initials: A
authors.lastname: Durak
authors.forename: Barbara
authors.initials: B
authors.lastname: Baraniak
authors.forename: Anna
authors.initials: A
authors.lastname: Jakubczyk
authors.forename: Michał
authors.initials: M
authors.lastname: Świeca
mesh_terms: D000806:Angiotensin-Converting Enzyme Inhibitors / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D055162:Biocatalysis; D002614:Chelating Agents / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D004063:Digestion; D004798:Enzymes / Q000378:metabolism*; D007887:Fabaceae / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D006868:Hydrolysis; D020128:Inhibitory Concentration 50; D008954:Models, Biological; D010455:Peptides / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D010940:Plant Proteins / Q000737:chemistry / Q000378:metabolism; D012639:Seeds / Q000737:chemistry / Q000378:metabolism
chemical_list: D000806:Angiotensin-Converting Enzyme Inhibitors; D000975:Antioxidants; D002614:Chelating Agents; D004798:Enzymes; D010455:Peptides; D010940:Plant Proteins
keywords: Adzuki bean; Biologically active peptides; Hydrolysates