title: Mast Cell Stabilizer Ketotifen Inhibits Gouty Inflammation in Rats.
abstract: Gout, an extremely painful arthritis with relapsing inflammatory attacks, is a common inflammatory joint disease in adults. We examined the therapeutic effect of ketotifen, a mast cell stabilizer, on monosodium urate (MSU) crystal-induced acute inflammation. Eight-week-old male Wistar rats were injected with MSU crystals (5 mg per rat) into air pouch. Ketotifen (0, 0.1, 03, and 1 mg/kg) was given 1 hour before MSU crystal injection. Lavage histamine, leukocyte counts, mast cell counts, nitric oxide, and proinflammatory mediator levels were assessed 12 hours after MSU injection. Ketotifen significantly inhibited MSU-induced mast cell activation and histamine concentration in air pouch lavage. Ketotifen dose-dependently inhibited MSU-initiated leukocyte infiltration into the air pouch. Furthermore, ketotifen significantly decreased proinflammatory mediators, including nitric oxide, interleukin-1β, and interleukin-6, production in MSU-treated rats. Ketotifen may attenuate MSU-induced acute inflammation by inhibiting mast cell activation and leukocyte infiltration in rats. Furthermore, ketotifen has the potential to be a new approach in managing patients with gouty inflammation in the future.
authors.affiliation: 1Departments of Environmental and Occupational Health, National Cheng Kung University Medical College, Tainan, Taiwan; and 2Sustainable Environment Research Centre, National Cheng Kung University, Tainan, Taiwan.
authors.forename: Dur-Zong
authors.initials: DZ
authors.lastname: Hsu
authors.forename: Pei-Yi
authors.initials: PY
authors.lastname: Chu
authors.forename: Si-Jin
authors.initials: SJ
authors.lastname: Chen
authors.forename: Ming-Yie
authors.initials: MY
authors.lastname: Liu
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage / Q000494:pharmacology*; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D006073:Gout / Q000139:chemically induced / Q000188:drug therapy*; D007249:Inflammation / Q000139:chemically induced / Q000188:drug therapy*; D018836:Inflammation Mediators / Q000378:metabolism*; D053583:Interleukin-1beta / Q000037:antagonists & inhibitors; D007665:Ketotifen / Q000008:administration & dosage / Q000494:pharmacology*; D007958:Leukocyte Count; D008297:Male; D008407:Mast Cells / Q000187:drug effects*; D009569:Nitric Oxide / Q000037:antagonists & inhibitors; D051381:Rats; D017207:Rats, Sprague-Dawley; D014527:Uric Acid / Q000494:pharmacology; D000093242:Interleukin-6 Inhibitors
chemical_list: D000893:Anti-Inflammatory Agents; D018836:Inflammation Mediators; D053583:Interleukin-1beta; D007665:Ketotifen; D009569:Nitric Oxide; D014527:Uric Acid; D000093242:Interleukin-6 Inhibitors