title: Electrochemical study of ellipticine interaction with single and double stranded oligonucleotides.
abstract: Ellipticine (5,11-dimethyl-6H-pyrido[4,3-b]carbazole) is an alkaloid that has been isolated from plants of an Apocynaceae family. It is one of the simplest naturally occurring alkaloids with a planar structure. Over the past decades, ellipticine became a very promising antitumor agent. Interaction with DNA is one of the most studied ellipticine effects on cell division. This phenomenon is not clearly explained so far. In our experiments we studied interaction of ellipticine with single-stranded and double-stranded oligonucleotides by electrochemical methods on mercury electrode. Differential pulse voltammetry was applied for ellipticine (Elli) and CA peak detection. Square wave voltammetry was applied for G peak detection. The effect of the interaction time and ellipticine concentrations on interactions of ellipticine with single- and double-stranded oligonucleotides was tested too.
authors.forename: Katerina
authors.initials: K
authors.lastname: Tmejova
authors.forename: Ludmila
authors.initials: L
authors.lastname: Krejcova
authors.forename: David
authors.initials: D
authors.lastname: Hynek
authors.forename: Vojtech
authors.initials: V
authors.lastname: Adam
authors.forename: Petr
authors.initials: P
authors.lastname: Babula
authors.forename: Libuse
authors.initials: L
authors.lastname: Trnkova
authors.forename: Marie
authors.initials: M
authors.lastname: Stiborova
authors.forename: Tomas
authors.initials: T
authors.lastname: Eckschlager
authors.affiliation: Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic, European Union. kizek@sci.muni.cz.
authors.forename: Rene
authors.initials: R
authors.lastname: Kizek
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry*; D029062:Apocynaceae / Q000737:chemistry; D055664:Electrochemical Techniques; D004611:Ellipticines / Q000737:chemistry*; D009841:Oligonucleotides / Q000737:chemistry*
chemical_list: D000970:Antineoplastic Agents; D004611:Ellipticines; D009841:Oligonucleotides; C034192:ellipticine