title: On-chip screening for prostate cancer: an EIS microfluidic platform for contemporary detection of free and total PSA.
abstract: Prostate cancer affects a large part of the western male population. The need for an early and accurate detection is thus a great challenge in common clinical practice, but the lack of specificity of the serum marker PSA (Prostate Specific Antigen) is a serious problem since its increased concentration can be related to several abnormalities. PSA, however, is found in serum in both a free and a complexed form with other proteins and the percentage amount of unbound PSA (the free-to-total PSA ratio) can be employed to distinguish prostate cancer from benign prostatic conditions, and also to predict the future risk of prostate cancer. To improve the operating characteristics of current PSA tests and to provide a clinical tool able to run label-free and sensitive analysis, we thus developed a biosensing platform based on Electrochemical Impedance Spectroscopy (EIS), which allows the contemporary detection of free and total PSA on a single biochip, enabling a quick screening for the risk of prostate cancer thanks to the presence of two different immobilized antibodies specific for the different antigens researched.
authors.affiliation: NNL Istituto Nanoscienze - CNR and Dipartimento di Matematica e Fisica Ennio De Giorgi, Università del Salento, Lecce, Italy. serena.chiriaco@unisalento.it
authors.forename: Maria Serena
authors.initials: MS
authors.lastname: Chiriacò
authors.forename: Elisabetta
authors.initials: E
authors.lastname: Primiceri
authors.forename: Alessandro
authors.initials: A
authors.lastname: Montanaro
authors.forename: Francesco
authors.initials: F
authors.lastname: de Feo
authors.forename: Lorenzo
authors.initials: L
authors.lastname: Leone
authors.forename: Ross
authors.initials: R
authors.lastname: Rinaldi
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Maruccio
mesh_terms: D058266:Dielectric Spectroscopy / Q000295:instrumentation*; D006801:Humans; D008297:Male; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D017430:Prostate-Specific Antigen / Q000032:analysis*; D011471:Prostatic Neoplasms / Q000175:diagnosis*
chemical_list: D017430:Prostate-Specific Antigen