title: 2-Selenouridine triphosphate synthesis and Se-RNA transcription.
abstract: 2-Selenouridine ((Se)U) is one of the naturally occurring modifications of Se-tRNAs ((Se)U-RNA) at the wobble position of the anticodon loop. Its role in the RNA-RNA interaction, especially during the mRNA decoding, is elusive. To assist the research exploration, herein we report the enzymatic synthesis of the (Se)U-RNA via 2-selenouridine triphosphate ((Se)UTP) synthesis and RNA transcription. Moreover, we have demonstrated that the synthesized (Se)UTP is stable and recognizable by T7 RNA polymerase. Under the optimized conditions, the transcription yield of (Se)U-RNA can reach up to 85% of the corresponding native RNA. Furthermore, the transcribed (Se)U-hammerhead ribozyme has the similar activity as the corresponding native, which suggests usefulness of (Se)U-RNAs in function and structure studies of noncoding RNAs, including the Se-tRNAs.
authors.affiliation: Department of Chemistry, Georgia State University, Atlanta, Georgia 30303, USA.
authors.forename: Huiyan
authors.initials: H
authors.lastname: Sun
authors.forename: Sibo
authors.initials: S
authors.lastname: Jiang
authors.forename: Julianne
authors.initials: J
authors.lastname: Caton-Williams
authors.forename: Hehua
authors.initials: H
authors.lastname: Liu
authors.forename: Zhen
authors.initials: Z
authors.lastname: Huang
mesh_terms: D000926:Anticodon; D002384:Catalysis; D012321:DNA-Directed RNA Polymerases / Q000737:chemistry / Q000235:genetics; D008958:Models, Molecular; D009690:Nucleic Acid Conformation; D016566:Organoselenium Compounds / Q000138:chemical synthesis* / Q000737:chemistry; D012313:RNA / Q000737:chemistry / Q000235:genetics*; D016337:RNA, Catalytic / Q000737:chemistry / Q000235:genetics; D012333:RNA, Messenger / Q000737:chemistry / Q000235:genetics; D014158:Transcription, Genetic; D014544:Uridine Triphosphate / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics
chemical_list: D000926:Anticodon; D016566:Organoselenium Compounds; D016337:RNA, Catalytic; D012333:RNA, Messenger; D014764:Viral Proteins; C427204:hammerhead ribozyme; D012313:RNA; C062210:bacteriophage T7 RNA polymerase; D012321:DNA-Directed RNA Polymerases; D014544:Uridine Triphosphate
keywords: 2-selenouridine triphosphate; RNA crystallography; RNA structure and function; RNA transcription; hammerhead ribozyme