title: Development and validation of RP-HPLC method for simultaneous estimation of glimepiride and sildenafil citrate in rat plasma-application to pharmacokinetic studies.
abstract: A simple and sensitive method was developed for simultaneous estimation of Glimepiride (GLIM) and Sildenafil citrate (SIL) in rat Plasma by reverse phase high performance liquid chromatography (RP-HPLC). The drug samples were extracted by liquid-liquid extraction with 300 µl of acetonitrile and 5 ml of diethyl ether. Chromatographic separation was achieved on C18 column using methanol: water (85:15 v/v) as mobile phase at a flow rate of 1 ml/min and UV detection at 230 nm. The retention time of GLIM and SIL was found to be 2.5 and 4.0 min respectively with total run time of 7 min. The developed method was validated for accuracy, precision, linearity and recovery. The method was linear and found to be acceptable over the range of 100-12 000 ng/ml. The method was successfully applied for the analysis of rat plasma sample for application to pharmacokinetic.
authors.affiliation: Department of Pharmacology, P. Wadhwani College of Pharmacy, Maharashtra, India.
authors.forename: A S
authors.initials: AS
authors.lastname: Tripathi
authors.forename: A P
authors.initials: AP
authors.lastname: Dewani
authors.forename: P G
authors.initials: PG
authors.lastname: Shelke
authors.forename: R L
authors.initials: RL
authors.lastname: Bakal
authors.forename: A V
authors.initials: AV
authors.lastname: Chandewar
authors.forename: P M
authors.initials: PM
authors.lastname: Mazumder
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D008297:Male; D010879:Piperazines / Q000032:analysis*; D011687:Purines / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D000068677:Sildenafil Citrate; D013450:Sulfones / Q000032:analysis*; D013453:Sulfonylurea Compounds / Q000032:analysis*
chemical_list: D010879:Piperazines; D011687:Purines; D013450:Sulfones; D013453:Sulfonylurea Compounds; C057619:glimepiride; D000068677:Sildenafil Citrate