title: Structural investigation of influenza virus hemagglutinin membrane-anchoring peptide.
abstract: Hemagglutinin (HA), the trimeric spike of influenza virus, catalyzes fusion of viral and cellular membranes. We have synthesized the anchoring peptide including the linker, transmembrane region and cytoplasmic tail (HA-TMR-CT) in a cell-free system. Furthermore, to mimic the palmitoylation of three conserved cysteines within the CT, we chemically alkylated HA-TMR-CT using hexadecyl-methanethiosulfonate. While the nuclear magnetic resonance spectroscopy showed pure and refolded peptides, the formation of multiple oligomers of higher order impeded further structural analysis. Circular dichroism spectroscopy of both alkylated and non-alkylated HA-TMR-CT revealed an α-helical secondary structure. No major impact of the fatty acids on the secondary structure was detected.
authors.affiliation: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya Street 16/10, 117997 Moscow, Russia.
authors.forename: Konstantin S
authors.initials: KS
authors.lastname: Mineev
authors.forename: Ekaterina N
authors.initials: EN
authors.lastname: Lyukmanova
authors.forename: Ludwig
authors.initials: L
authors.lastname: Krabben
authors.forename: Marina V
authors.initials: MV
authors.lastname: Serebryakova
authors.forename: Mikhail A
authors.initials: MA
authors.lastname: Shulepko
authors.forename: Alexander S
authors.initials: AS
authors.lastname: Arseniev
authors.forename: Larisa V
authors.initials: LV
authors.lastname: Kordyukova
authors.forename: Michael
authors.initials: M
authors.lastname: Veit
mesh_terms: D000478:Alkylation; D000595:Amino Acid Sequence; D004926:Escherichia coli / Q000378:metabolism; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000096:biosynthesis / Q000737:chemistry*; D057916:Influenza A Virus, H7N1 Subtype / Q000737:chemistry* / Q000235:genetics; D054878:Lipoylation; D008698:Mesylates / Q000737:chemistry; D018716:Molecular Mimicry; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry* / Q000378:metabolism; D017510:Protein Folding; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D019267:Hemagglutinin Glycoproteins, Influenza Virus; D008698:Mesylates; D010455:Peptides; D011994:Recombinant Proteins
keywords: acylation; cytoplasmic tail; hemagglutinin; influenza virus; palmitoylation; transmembrane region