title: Impact of surface nano-textured stainless steel prepared by focused ion beam on endothelial cell growth.
abstract: The modification of stent surfaces with nano-structures has the potential for limiting late stent restenosis. We report here the patterning of 316L austentitic stainless steel with arrays of nano-pits of two nominal diameters: 120 and 180 nm. These nano-textured surfaces were prepared by focused ion beam milling. The influence of the ion beam current on the nano-features was investigated by scanning electron and atomic force microscopies. The optimum ion beam currents were 280 pA for 120 nm nano-pits and 920 pA for 180 nm nano-pits. The depths of the nano-pits formed were (65 +/- 24) nm (120 nm) and (84 +/- 36) nm (180 nm). This wide distribution of the depths is due to the polycrystalline nature of 316 L stainless steel, which has a strong influence on the milling rates. Endothelial cells were grown in vitro on these substrates for 1, 3 and 5 days. The cells were viable for the duration of the cell culture on the nano-textured substrates. There was no significant difference in the adhesion and the proliferation based on the nano-pit diameter.
authors.affiliation: Tyndall National Institute, University College, Cork, Ireland.
authors.forename: Feroze
authors.initials: F
authors.lastname: Nazneen
authors.forename: Michael
authors.initials: M
authors.lastname: Schmidt
authors.forename: Eve
authors.initials: E
authors.lastname: McLoughlin
authors.forename: Nikolay
authors.initials: N
authors.lastname: Petkov
authors.forename: Grégoire
authors.initials: G
authors.lastname: Herzog
authors.forename: Damien W M
authors.initials: DW
authors.lastname: Arrigan
authors.forename: Paul
authors.initials: P
authors.lastname: Galvin
mesh_terms: D002448:Cell Adhesion; D018929:Cell Culture Techniques; D002470:Cell Survival; D003460:Crystallization; D003470:Culture Media / Q000737:chemistry; D042783:Endothelial Cells / Q000166:cytology*; D004815:Epidermal Growth Factor / Q000378:metabolism; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D007477:Ions / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D008855:Microscopy, Electron, Scanning; D036103:Nanotechnology / Q000379:methods*; D013193:Stainless Steel / Q000737:chemistry*; D013499:Surface Properties; D013997:Time Factors
chemical_list: D003470:Culture Media; D007477:Ions; D013193:Stainless Steel; D004815:Epidermal Growth Factor