title: Isolation, characterization, and neuroprotective activities of sesquiterpenes from Petasites japonicus.
abstract: Neuroprotective reagents to protect the nerve cells against oxidative stress and other damages are potentially effective for the medical treatment of Parkinson's disease. Petasites japonicus, a wild vegetable, belongs to the family Compositae and its extract has shown the neuroprotective effects. A further phytochemical investigation of P. japonicus for neuroprotective substances led to the isolation of eight new (1-8) and two known (9 and 10) sesquiterpenes. Their structures were elucidated on the basis of extensive 1D and 2D NMR (HMQC, HMBC, (1)H-(1)H COSY, and NOESY) spectroscopic data analyses, and the structure of 1 was confirmed by X-ray crystallography. The neuroprotective activities of these sesquiterpenes were evaluated against cobalt chloride (CoCl2)-induced neuronal cell death in human dopaminergic SH-SY5Y cells. Five compounds showed a neuroprotective activity.
authors.affiliation: College of Pharmacy, State Key Laboratory of Medicinal Chemical Biology and Tianjin Key Laboratory of Molecular Drug Research, Nankai University, Tianjin 300071, People's Republic of China.
authors.forename: Shaonan
authors.initials: S
authors.lastname: Wang
authors.forename: Da-Qing
authors.initials: DQ
authors.lastname: Jin
authors.forename: Chunfeng
authors.initials: C
authors.lastname: Xie
authors.forename: Hao
authors.initials: H
authors.lastname: Wang
authors.forename: Meicheng
authors.initials: M
authors.lastname: Wang
authors.forename: Jing
authors.initials: J
authors.lastname: Xu
authors.forename: Yuanqiang
authors.initials: Y
authors.lastname: Guo
mesh_terms: D002460:Cell Line; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D015394:Molecular Structure; D018696:Neuroprotective Agents / Q000737:chemistry* / Q000494:pharmacology*; D036901:Petasites / Q000737:chemistry*; D012717:Sesquiterpenes / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D004365:Drugs, Chinese Herbal; D018696:Neuroprotective Agents; D012717:Sesquiterpenes
keywords: Neuroprotective activities; Parkinson’s disease; Petasites japonicus; Sesquiterpenes