title: Interaction study of acetylcholinestrase inhibitors on pharmacokinetics of memantine in rat plasma by HPLC-fluorescence method.
abstract: The present study aims to investigate the possibility of interaction of donepezil (DP) and galantamine (GAL) as acetylcholinestrase inhibitors, on memantine (MT) hydrochloride in rat plasma by HPLC-fluorescence detection. The separation of MT was achieved within 12 min without interference of DP and GAL on the chromatogram. MT levels in rat plasma with a single administration of MT (2.5 mg/kg, i.p.) and those with a co-administration of DP (5.0 mg/kg, i.p.) and GAL (3 mg/kg, i.p.) were monitored. MT concentrations determined in rat plasma ranged from 10.0 to 245.6 ng/mL. Significant difference was observed in the behavior of MT with a co-administration of DP, while no significant difference was observed with a co-administration of GAL.
authors.affiliation: Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Al-Azhar University, Assiut branch, Assiut, 71524, Egypt; Graduate School of Biomedical Sciences, Nagasaki University, 1-14 Bunkyo-machi, Nagasaki, 852-8521, Japan.
authors.forename: Mohamed G
authors.initials: MG
authors.lastname: Hassan
authors.forename: Rie
authors.initials: R
authors.lastname: Ikeda
authors.forename: Mitsuhiro
authors.initials: M
authors.lastname: Wada
authors.forename: Naotaka
authors.initials: N
authors.lastname: Kuroda
authors.forename: Hanaa M
authors.initials: HM
authors.lastname: Abdel-Wadood
authors.forename: Horria A
authors.initials: HA
authors.lastname: Mohamed
authors.forename: Kenichiro
authors.initials: K
authors.lastname: Nakashima
mesh_terms: D000818:Animals; D002800:Cholinesterase Inhibitors / Q000008:administration & dosage / Q000493:pharmacokinetics*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D000077265:Donepezil; D004347:Drug Interactions; D005702:Galantamine / Q000008:administration & dosage / Q000493:pharmacokinetics; D007189:Indans / Q000008:administration & dosage / Q000493:pharmacokinetics; D008297:Male; D008559:Memantine / Q000008:administration & dosage / Q000097:blood / Q000493:pharmacokinetics*; D010880:Piperidines / Q000008:administration & dosage / Q000493:pharmacokinetics; D051381:Rats; D017208:Rats, Wistar; D013050:Spectrometry, Fluorescence / Q000379:methods
chemical_list: D002800:Cholinesterase Inhibitors; D007189:Indans; D010880:Piperidines; D005702:Galantamine; D000077265:Donepezil; D008559:Memantine
keywords: HPLC-fluorescence detection; acetylcholinestrase inhibitors; memantine; pharmacokinetics