title: Myo10 is a key regulator of TNT formation in neuronal cells.
abstract: Cell-to-cell communication is essential in multicellular organisms. Tunneling nanotubes (TNTs) have emerged as a new type of intercellular spreading mechanism allowing the transport of various signals, organelles and pathogens. Here, we study the role of the unconventional molecular motor myosin-X (Myo10) in the formation of functional TNTs within neuronal CAD cells. Myo10 protein expression increases the number of TNTs and the transfer of vesicles between co-cultured cells. We also show that TNT formation requires both the motor and tail domains of the protein, and identify the F2 lobe of the FERM domain within the Myo10 tail as necessary for TNT formation. Taken together, these results indicate that, in neuronal cells, TNTs can arise from a subset of Myo10-driven dorsal filopodia, independent of its binding to integrins and N-cadherins. In addition our data highlight the existence of different mechanisms for the establishment and regulation of TNTs in neuronal cells and other cell types.
authors.affiliation: Institut Pasteur, 25 Rue du Dr Roux, Unité de Traffic Membranaire et Pathogenèse, 75724 Paris Cedex 15, France.
authors.forename: Karine
authors.initials: K
authors.lastname: Gousset
authors.forename: Ludovica
authors.initials: L
authors.lastname: Marzo
authors.forename: Pierre-Henri
authors.initials: PH
authors.lastname: Commere
authors.forename: Chiara
authors.initials: C
authors.lastname: Zurzolo
mesh_terms: D000818:Animals; D001692:Biological Transport; D002450:Cell Communication / Q000502:physiology*; D051379:Mice; D009218:Myosins / Q000378:metabolism*; D043942:Nanotubes; D009474:Neurons / Q000166:cytology* / Q000378:metabolism*; D011554:Pseudopodia / Q000378:metabolism; D014162:Transfection
chemical_list: C409064:Myo10 protein, mouse; D009218:Myosins
keywords: Dorsal filopodia; FERM domain; Intercellular transfer; Myo10; TNTs