title: Bovine papillomavirus type 2 infection and a series of mesenchymal tumors of the urinary bladder in cattle.
abstract: This report describes the histopathology of two hundred and fifty-three mesenchymal tumors of the urinary bladder in cattle grazing on lands rich in bracken fern. Approximately 80% were hemangiomas and angiosarcomas. Hemangioma (capillary, cavernous, and large vessels) was the most frequent mesenchymal tumor and was more common than angiosarcoma. Although the appearance of endothelial cells can vary remarkably, epithelioid angiosarcomas, often containing multinucleated cells, were the most frequent malignant vascular tumors. Hemangiopericytoma and tumors of muscle and soft connective tissue origin, alone and/or in association with tumor-like lesions, were less frequently seen. Furthermore, forty-five cases of intravascular papillary endothelial hyperplasia (IPEH), a lesion not previously reported in the urinary bladder of cattle, were also described. Bovine papillomavirus type-2 DNA was amplified in tumor samples. Forty vascular tumors were investigated by dual-labeling immunofluorescence, and, for the first time, a coexpression of E5 and platelet-derived growth factor β receptor (PDGF β R) was shown to occur. The results show that the BPV-2 E5 oncoprotein binds to the activated form of the PDGF β receptor thus playing an important role in mesenchymal as well as epithelial carcinogenesis of the urinary bladder. Furthermore, these findings demonstrate that BPV-2 infects both epithelial and mesenchymal cells.
authors.affiliation: Department of Veterinary Medicine and Animal Productions, Naples University Federico II, Naples, Italy.
authors.forename: Manuela
authors.initials: M
authors.lastname: Martano
authors.forename: Franco
authors.initials: F
authors.lastname: Roperto
authors.forename: Rita de Cassia
authors.initials: Rde C
authors.lastname: Stocco
authors.forename: Valeria
authors.initials: V
authors.lastname: Russo
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Borzacchiello
authors.forename: Orlando
authors.initials: O
authors.lastname: Paciello
authors.forename: Valentina
authors.initials: V
authors.lastname: Iovane
authors.forename: Leonardo
authors.initials: L
authors.lastname: Leonardi
authors.forename: Paola
authors.initials: P
authors.lastname: Maiolino
authors.forename: Brunella
authors.initials: B
authors.lastname: Restucci
authors.forename: Serenella
authors.initials: S
authors.lastname: Papparella
authors.forename: Sante
authors.initials: S
authors.lastname: Roperto
mesh_terms: D000818:Animals; D001808:Blood Vessels / Q000473:pathology; D052688:Bovine papillomavirus 1 / Q000502:physiology*; D002417:Cattle; D002418:Cattle Diseases / Q000473:pathology* / Q000821:virology*; D006965:Hyperplasia; D008637:Mesenchymoma / Q000473:pathology / Q000662:veterinary* / Q000821:virology; D009363:Neoplasm Proteins / Q000378:metabolism; D030361:Papillomavirus Infections / Q000473:pathology / Q000662:veterinary* / Q000821:virology; D020797:Receptor, Platelet-Derived Growth Factor beta / Q000378:metabolism; D001743:Urinary Bladder / Q000473:pathology* / Q000821:virology*
chemical_list: D009363:Neoplasm Proteins; D020797:Receptor, Platelet-Derived Growth Factor beta