title: Biodegradation of anthracene by a novel actinomycete, Microbacterium sp. isolated from tropical hydrocarbon-contaminated soil.
abstract: A novel anthracene-degrading Gram-positive actinomycete, Microbacterium sp. strain SL10 was isolated from a hydrocarbon-contaminated soil at a mechanical engineering workshop in Lagos, Nigeria. The polluted soil had an unusually high total hydrocarbon content of 157 g/kg and presence of various heavy metals. The isolate tolerated salt concentration of more than 4%. It resisted cefotaxime, streptomycin and ciprofloxacin, but susceptible to meropenem, linezolid and vancomycin. The isolate exhibited growth rate and doubling time of 0.82 days(-1) and 0.84 days, respectively on anthracene. It degraded 57.5 and 90.12% of anthracene within 12 and 21 days, respectively while the rate of anthracene utilization by the isolate was 4.79 mg l(-1) d(-1). To the best of our knowledge, this is the first report of isolation and characterization of anthracene-degrading Microbacterium sp.
authors.affiliation: Department of Microbiology, Faculty of Science, Lagos State University, Ojo, Lagos, Nigeria, babssalaam@yahoo.com.
authors.forename: Lateef B
authors.initials: LB
authors.lastname: Salam
authors.forename: Oluwafemi S
authors.initials: OS
authors.lastname: Obayori
authors.forename: Nojeem O
authors.initials: NO
authors.lastname: Olatoye
mesh_terms: D000192:Actinomycetales / Q000187:drug effects / Q000254:growth & development / Q000302:isolation & purification* / Q000378:metabolism*; D000873:Anthracenes / Q000378:metabolism*; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001711:Biotransformation; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D006838:Hydrocarbons / Q000032:analysis; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D009549:Nigeria; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012987:Soil / Q000737:chemistry; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000032:analysis; D014329:Tropical Climate
chemical_list: D000873:Anthracenes; D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D006838:Hydrocarbons; D012336:RNA, Ribosomal, 16S; D012987:Soil; D012989:Soil Pollutants; C034020:anthracene