title: Different Sarcocystis spp. are present in bovine eosinophilic myositis.
abstract: It has been suggested that Sarcocystis species are associated with bovine eosinophilic myositis (BEM). To date, parasite identification in this myopathy has been based on morphological techniques. The aim of the present study was to use molecular techniques to identify Sarcocystis species inside lesions of BEM. Histologically, BEM lesions of 97 condemned carcasses were examined for the presence of Sarcocystis species. Intralesional and extralesional cysts were collected using laser capture microdissection and the species was determined with a PCR-based technique based on 18S rDNA. Intralesional sarcocysts or remnants were found in BEM lesions in 28% of the carcasses. The majority (82%) of intralesional Sarcocystis species were found to be S. hominis. However S. cruzi and S. hirsuta were also found, as well as an unidentified species. It can be concluded that Sarcocystis species present in lesions of BEM are not restricted to one species.
authors.affiliation: Laboratory of Veterinary Pathology, Department of Pathology, Bacteriology and Avian Diseases, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, Belgium.
authors.forename: Lieve
authors.initials: L
authors.lastname: Vangeel
authors.forename: Kurt
authors.initials: K
authors.lastname: Houf
authors.forename: Peter
authors.initials: P
authors.lastname: Geldhof
authors.forename: Katleen
authors.initials: K
authors.lastname: De Preter
authors.forename: Jozef
authors.initials: J
authors.lastname: Vercruysse
authors.forename: Richard
authors.initials: R
authors.lastname: Ducatelle
authors.forename: Koen
authors.initials: K
authors.lastname: Chiers
mesh_terms: D000818:Animals; D001483:Base Sequence; D002417:Cattle; D002418:Cattle Diseases / Q000469:parasitology*; D016054:DNA, Protozoan / Q000235:genetics; D008969:Molecular Sequence Data; D009220:Myositis / Q000469:parasitology / Q000473:pathology / Q000662:veterinary*; D010802:Phylogeny; D012522:Sarcocystis / Q000302:isolation & purification*; D012523:Sarcocystosis / Q000469:parasitology / Q000473:pathology / Q000662:veterinary*; D013045:Species Specificity
chemical_list: D016054:DNA, Protozoan
keywords: Bovine eosinophilic myositis; Cattle; Laser capture microdissection; Molecular identification; Sarcocystis species