title: Direct observation of CD4 T cell morphologies and their cross-sectional traction force derivation on quartz nanopillar substrates using focused ion beam technique.
abstract: Direct observations of the primary mouse CD4 T cell morphologies, e.g., cell adhesion and cell spreading by culturing CD4 T cells in a short period of incubation (e.g., 20 min) on streptavidin-functionalized quartz nanopillar arrays (QNPA) using a high-content scanning electron microscopy method were reported. Furthermore, we first demonstrated cross-sectional cell traction force distribution of surface-bound CD4 T cells on QNPA substrates by culturing the cells on top of the QNPA and further analysis in deflection of underlying QNPA via focused ion beam-assisted technique.
authors.affiliation: Department of Physics, Chung-Ang University, Seoul 156-756, Republic of Korea. sangkwonlee@cau.ac.kr.
authors.forename: Dong-Joo
authors.initials: DJ
authors.lastname: Kim
authors.forename: Gil-Sung
authors.initials: GS
authors.lastname: Kim
authors.forename: Jung-Hwan
authors.initials: JH
authors.lastname: Hyung
authors.forename: Won-Yong
authors.initials: WY
authors.lastname: Lee
authors.forename: Chang-Hee
authors.initials: CH
authors.lastname: Hong
authors.forename: Sang-Kwon
authors.initials: SK
authors.lastname: Lee