title: Monocyte human leukocyte antigen-DR expression-a tool to distinguish intestinal bacterial infections from inflammatory bowel disease?
abstract: BACKGROUND
  We sought to determine the quantitative expression of human leukocyte antigen-DR (HLA-DR) on monocytes in patients with acute intestinal bacterial infections and inflammatory bowel disease (IBD).
  
  
  METHODS
  The quantitative expression of HLA-DR on monocytes was determined by fluorescence-activated cell sorting analysis in patients with IBD, patients with acute intestinal bacterial infections (bact.), and healthy subjects (contr.).
  
  
  RESULTS
  The quantitative expression of HLA-DR in patients with bact. (n = 20; 90,000 molecules per monocyte; confidence interval [CI], 79,000-102,000) was significantly higher than that in patients with ulcerative colitis (n = 40, 30,000; CI, 30,000-38,000; P < 0.0001), Crohn disease (n = 80, 31,000; CI, 32,000-39,000; P < 0.0001), or in contr. (n = 28, 39,000; CI, 36,000-46,000; P < 0.0001). In patients with ulcerative colitis and Crohn disease, HLA-DR expression was significantly decreased, as compared with contr. (P < 0.05 and P < 0.01, respectively). With a cutoff point of 50,000, HLA-DR showed a sensitivity of 95% and a specificity of 92% in discriminating between bact. and active IBD.
  
  
  CONCLUSIONS
  The quantitative measurement of HLA-DR expression could serve as a valuable tool to discriminate between bact. and active IBD.
authors.affiliation: Hartmann Hospital, Vienna, Austria. wtillinger@hartmannspital.at
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Tillinger
authors.forename: Ruth
authors.initials: R
authors.lastname: Jilch
authors.forename: Thomas
authors.initials: T
authors.lastname: Waldhoer
authors.forename: Walter
authors.initials: W
authors.lastname: Reinisch
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Junger
mesh_terms: D000328:Adult; D001424:Bacterial Infections / Q000276:immunology*; D005260:Female; D005434:Flow Cytometry; D006684:HLA-DR Antigens / Q000378:metabolism*; D006801:Humans; D015212:Inflammatory Bowel Diseases / Q000175:diagnosis* / Q000276:immunology*; D007422:Intestines / Q000276:immunology* / Q000382:microbiology*; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000276:immunology* / Q000378:metabolism*; D055815:Young Adult
chemical_list: D006684:HLA-DR Antigens