title: Characterisation and analysis of thioredoxin peroxidase as a potential antigen for the serodiagnosis of sarcoptic mange in rabbits by dot-ELISA.
abstract: BACKGROUND
  Scabies caused by Sarcoptes scabiei is a widespread but a neglected tropical zoonosis. In this study, we characterised a S. scabiei thioredoxin peroxidase (SsTPx) and evaluated a recombinant SsTPx as a diagnostic antigen in rabbits.
  
  
  METHODS
  The open reading frame of the gene encoding SsTPx-2 was amplified and the recombinant protein was expressed in Escherichia coli cells and purified. SsTPx was localized in mite tissue by immunolocalisation using the purified recombinant protein. Serodiagnosis assays were carried out in 203 New Zealand White rabbit serum samples by dot-ELISA.
  
  
  RESULTS
  The open reading frame (489 bp) of the gene encodes an 18.11 kDa protein, which showed highly homology to that of Psoroptes cuniculi (98.77% identity) and belongs to the 2-Cys family of peroxiredoxins. SsTPx was mainly distributed in muscle tissues of mites, integument of the epidermis and the anterior end of S. scabiei. Although SsTPx cross-reactivity with psoroptic mites was observed, the SsTPx dot-ELISA showed excellent diagnostic ability, with 95.3% sensitivity and 93.8% specificity in mange-infected and uninfected groups.
  
  
  CONCLUSIONS
  This study showed that the purified SsTPx is a highly sensitive antigen for the diagnosis of mange infection by dot-ELISA. This technique is a rapid and convenient method that can be used worldwide for the clinical diagnosis of sarcoptic mange in rabbits, and is especially useful in developing regions.
authors.affiliation: Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China.
authors.forename: Runhui
authors.initials: R
authors.lastname: Zhang
authors.forename: Wanpeng
authors.initials: W
authors.lastname: Zheng
authors.forename: Xuhang
authors.initials: X
authors.lastname: Wu
authors.forename: Quwu
authors.initials: Q
authors.lastname: Jise
authors.forename: Yongjun
authors.initials: Y
authors.lastname: Ren
authors.forename: Xiang
authors.initials: X
authors.lastname: Nong
authors.forename: Xiaobin
authors.initials: X
authors.lastname: Gu
authors.forename: Shuxian
authors.initials: S
authors.lastname: Wang
authors.forename: Xuerong
authors.initials: X
authors.lastname: Peng
authors.forename: Songjia
authors.initials: S
authors.lastname: Lai
authors.forename: Guangyou
authors.initials: G
authors.lastname: Yang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary*; D054464:Peroxiredoxins / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D011817:Rabbits / Q000469:parasitology*; D012520:Sarcoptes scabiei / Q000201:enzymology* / Q000235:genetics; D012532:Scabies / Q000175:diagnosis / Q000662:veterinary*; D016415:Sequence Alignment; D020539:Sequence Analysis, Protein; D012698:Serologic Tests / Q000379:methods / Q000662:veterinary
chemical_list: D054464:Peroxiredoxins