title: Activation of Egr-1 in human lung epithelial cells exposed to silica through MAPKs signaling pathways.
abstract: The alveolar type II epithelial cell, regarded historically as a key target cell in initial injury by silica, now appears to be important in both defense from lung damage as well as elaboration of chemokines and cytokines. The molecular basis for silica-induced epithelial cell injury is poorly understood. In this study we explored the activation of nuclear factor Egr-1 and related signal pathway. Human II alveolar epithelial line A549 cells were exposed to silica for indicated time to assay the expression and activation of Egr-1 and upstream MAPKs. Immunofluorescence, western-blot techniques, RT-PCR, Electrophoretic mobility shift assay (EMSA), transient transfection assay, kinase inhibitor experiments were performed. It was found that the expression of Egr-1 at mRNA and protein level was significantly increased in A549 cells after administration with silica and the activity of Egr-1 peaked by silica treatment for 60 minutes. Furthermore, phosphorylated-ERK1/2, P38 MAPKs (the upstream kinase of Egr-1) ballooned during 15-30minutes, 30-60minutes respectively after silica exposure in A549 cells. By administration of ERK1/2, P38 inhibitor, the expression and transcription of Egr-1 were both markedly decreased. But PKC inhibitor did not prevent the increase of Egr-1. These results indicated Egr-1 played a critical role in silica-induced pulmonary fibrosis in an ERK1/2, P38 MAPKs-dependent manner, which suggests Egr-1 is an essential regulator in silicosis, and underlines a new molecular mechanism for fibrosis induced by silica.
authors.affiliation: Department of Pathology, Third Xiangya hospital, Central South University, Changsha, Hunan, PR China.
authors.forename: Ling
authors.initials: L
authors.lastname: Chu
authors.forename: Tiansheng
authors.initials: T
authors.lastname: Wang
authors.forename: Yongbin
authors.initials: Y
authors.lastname: Hu
authors.forename: Yonghong
authors.initials: Y
authors.lastname: Gu
authors.forename: Zanshan
authors.initials: Z
authors.lastname: Su
authors.forename: Haiying
authors.initials: H
authors.lastname: Jiang
mesh_terms: D015153:Blotting, Western; D002460:Cell Line; D051766:Early Growth Response Protein 1 / Q000378:metabolism*; D024202:Electrophoretic Mobility Shift Assay; D004847:Epithelial Cells / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D005786:Gene Expression Regulation / Q000187:drug effects*; D006801:Humans; D020935:MAP Kinase Signaling System / Q000187:drug effects*; D011650:Pulmonary Alveoli / Q000166:cytology / Q000293:injuries*; D011658:Pulmonary Fibrosis / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012822:Silicon Dioxide / Q000633:toxicity*; D013997:Time Factors
chemical_list: C497325:EGR1 protein, human; D051766:Early Growth Response Protein 1; D012822:Silicon Dioxide