title: Peroxidase-linked assay for detection of antibodies against bovine leukosis virus.
abstract: A peroxidase linked assay (PLA) was designed to screen bovine sera for the presence of specific antibodies against bovine leukosis virus (BLV). Out of 201 samples of bovine sera analyzed, 52.2% were considered positive by PLA, 26.4% by AGID, and 38.9% by ELISA. Western blotting analyses excluded 27 samples found to be positive by PLA. PLA showed 100% of sensitivity when compared with AGID and ELISA. Specificity was 64.8% and 78%, respectively (kappa coefficients were 0.70 and 0.83). These findings indicate that PLA can be used as an alternative method for the diagnosis of BLV infection in cattle.
authors.affiliation: Laboratory of Virology and Immunology, Universidade Federal de Pelotas, Pelotas, Brazil.
authors.forename: Clarissa C
authors.initials: CC
authors.lastname: de Castro
authors.forename: Cristina F
authors.initials: CF
authors.lastname: Nunes
authors.forename: Paula F
authors.initials: PF
authors.lastname: Finger
authors.forename: Bianca S
authors.initials: BS
authors.lastname: Siedler
authors.forename: Luana
authors.initials: L
authors.lastname: Dummer
authors.forename: Marcelo
authors.initials: M
authors.lastname: de Lima
authors.forename: Fábio P L
authors.initials: FP
authors.lastname: Leite
authors.forename: Geferson
authors.initials: G
authors.lastname: Fischer
authors.forename: Gilberto D'A
authors.initials: GD
authors.lastname: Vargas
authors.forename: Silvia de O
authors.initials: Sde O
authors.lastname: Hübner
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D002417:Cattle; D016583:Enzootic Bovine Leukosis / Q000175:diagnosis* / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary*; D005779:Immunodiffusion / Q000379:methods; D001909:Leukemia Virus, Bovine / Q000276:immunology*; D009195:Peroxidase / Q000276:immunology*; D012680:Sensitivity and Specificity
chemical_list: D000914:Antibodies, Viral; D009195:Peroxidase