title: Genetic analysis of ADIPOQ variants and gastric cancer risk: a hospital-based case-control study in China.
abstract: Single-nucleotide polymorphisms (SNPs) of adiponectin (ADIPOQ), adiponectin receptor 1 (ADIPOR1) and ADIPOR2 genes contribute to the risk and progression of cancers. Here, we investigated the associations between variants of these three genes and the risk of gastric cancer. We genotyped six ADIPOQ SNPs, nine ADIPOR1 SNPs and six ADIPOR2 SNPs using the Sequenom technique in a hospital-based case-control study of patients with gastric cancer and cancer-free controls in the Chinese Han population. We found associations of certain variants with location of gastric cancer. Rs16861205 with the minor allele A in ADIPOQ, rs10773989 with the minor allele C and rs1044471 with the minor allele T in ADIPOR2 presented significant associations with a decreased risk of cardia cancer (P = 0.024, OR 0.605, 95 % CI 0.390-0.938; P = 0.015, OR 0.699, 95 % CI 0.522-0.935; and P = 0.022, OR = 0.703, 95 % CI 0.519-0.951, respectively). ADIPOQ rs16861205 with minor allele A displayed an association with an increased risk of body cancer (P = 0.010, OR 1.821, 95 % CI 1.148-2.890). Further stratified analysis of the patients indicated that there were significant correlations for rs1342387A/G (P = 0.027) and rs16861205A/G (P = 0.000) with tumor location; rs16850799A/G (P = 0.004) and rs2058033C/A (P = 0.003) with invasion depth; rs16850799A/G (P = 0.019) with the tumor-node-metastasis stage; rs16850799A/G (P = 0.016), rs1501299A/C (P = 0.005) and rs1063538C/T (P = 0.017) with alcohol consumption; rs11612414A/G (P = 0.040) and rs12733285T/C (P = 0.005) with salted food; rs1063538C/T (P = 0.043) with family history of gastric cancer; and rs11612414A/G (P = 0.029) with gender. Adiponectin expression significantly correlated with gender (P = 0.014), alcohol consumption (P = 0.037), family history (P = 0.019) and invasion depth of primary tumor (P = 0.024). Our data suggested that variants of ADIPOQ may be genetic markers conferring susceptibility to gastric cancer subtypes. These findings need to be validated in a larger panel of samples from distinct populations.
authors.affiliation: Department of Pathology, School of Life Sciences, Anhui Medical University, Hefei, 230032, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Ye
authors.forename: Zuo-Yang
authors.initials: ZY
authors.lastname: Zhang
authors.forename: Wei-Dong
authors.initials: WD
authors.lastname: Du
authors.forename: Marion E
authors.initials: ME
authors.lastname: Schneider
authors.forename: Yue
authors.initials: Y
authors.lastname: Qiu
authors.forename: Yuan
authors.initials: Y
authors.lastname: Zhou
authors.forename: Fu-Sheng
authors.initials: FS
authors.lastname: Zhou
authors.forename: Xian-Bo
authors.initials: XB
authors.lastname: Zuo
authors.forename: Gang
authors.initials: G
authors.lastname: Chen
authors.forename: Xue-Ling
authors.initials: XL
authors.lastname: Ma
authors.forename: Hong-Zhen
authors.initials: HZ
authors.lastname: Xia
authors.forename: Song
authors.initials: S
authors.lastname: Xu
authors.forename: Ji-Feng
authors.initials: JF
authors.lastname: Wu
authors.forename: Hong-Yuan
authors.initials: HY
authors.lastname: Xu
authors.forename: Xue-Jun
authors.initials: XJ
authors.lastname: Zhang
mesh_terms: D052242:Adiponectin / Q000235:genetics*; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000483:Alleles; D044466:Asian People / Q000235:genetics; D014408:Biomarkers, Tumor / Q000235:genetics; D016022:Case-Control Studies; D005260:Female; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D054419:Receptors, Adiponectin / Q000235:genetics; D012306:Risk; D013274:Stomach Neoplasms / Q000209:etiology / Q000235:genetics*; D055815:Young Adult
chemical_list: C499918:ADIPOQ protein, human; C475418:ADIPOR1 protein, human; C475420:ADIPOR2 protein, human; D052242:Adiponectin; D014408:Biomarkers, Tumor; D054419:Receptors, Adiponectin