title: Evaluation of hydro-alcoholic extract of Eclipta alba for its multidrug resistance reversal potential: an in vitro study.
abstract: The development of multidrug resistance (MDR) causes problems in the chemotherapy of human cancer. The present study was designed to evaluate and establish the role of Eclipta alba as MDR reversal agent using multidrug resistant hepatocellular carcinoma cell line (DR-HepG2). To develop DR-HepG2, hepatocellular carcinoma cell line (HepG2) was transfected with 2-Acetylaminofluorene (AAF) and Aflatoxin B1 (AFB). Cytotoxic effects of the Eclipta alba hydroalcoholic extract (EAE) and standard anti-ancer drug Doxorubicin (DOX) were determined in DR-HepG2 and the parental cells HepG2 using MTT assay. The expression level of MDR1 gene and P-glycoprotein (P-gp) level was analyzed by RT-PCR and western blotting. From the present investigation, it was found that EAE (10 and 20 μg/ml) could significantly inhibit cell proliferation in DR-HepG2 whereas DOX (0.5 μg/ml) could not because of enhancement effect of MDR1/P-gp. This study demonstrated for the first time the antiproliferative activities of EAE in multidrug resistant DR-HepG2 cells. The findings revealed that Eclipta alba components are effective inhibitors of MDR1/P-gp.
authors.affiliation: Institute of Science, Nirma University, Chharodi, Ahmedabad, Gujarat, India.
authors.forename: Harshita
authors.initials: H
authors.lastname: Chaudhary
authors.forename: Prasant Kumar
authors.initials: PK
authors.lastname: Jena
authors.forename: Sriram
authors.initials: S
authors.lastname: Seshadri
mesh_terms: D015073:2-Acetylaminofluorene / Q000494:pharmacology; D018435:ATP Binding Cassette Transporter, Subfamily B; D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000235:genetics / Q000378:metabolism*; D016604:Aflatoxin B1 / Q000494:pharmacology; D006528:Carcinoma, Hepatocellular / Q000188:drug therapy; D049109:Cell Proliferation / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D004317:Doxorubicin / Q000494:pharmacology; D019008:Drug Resistance, Neoplasm / Q000187:drug effects*; D036601:Eclipta / Q000737:chemistry*; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D056945:Hep G2 Cells; D006801:Humans; D059625:Liquid-Liquid Extraction; D008113:Liver Neoplasms / Q000188:drug therapy; D009369:Neoplasms / Q000235:genetics / Q000378:metabolism*; D010936:Plant Extracts / Q000494:pharmacology*; D014162:Transfection; D015262:Xenobiotics / Q000494:pharmacology
chemical_list: C513055:ABCB1 protein, human; D018435:ATP Binding Cassette Transporter, Subfamily B; D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D010936:Plant Extracts; D015262:Xenobiotics; D004317:Doxorubicin; D015073:2-Acetylaminofluorene; D016604:Aflatoxin B1