title: Intracellular proteins of ethanol-treated yeast cells involved in iron sorption.
abstract: Iron is essential for human health, but it sometimes causes an unpleasant taste, rusty colour and a decrease in the stability of food products. Previously, we found that ethanol-treated yeast (ETY) cells could remove iron from wine and juice, and reduce the fishy aftertaste induced by iron in wine-seafood pairings. However, the mechanism of iron sorption by ETY cells is undefined; thus, there is no indicator that can be used to estimate the iron sorption capacity of these cells. In this study, we showed that cell wall components are not mainly associated with iron sorption by investigating ETY cells with the cell wall removed. Moreover, plasma membrane permeability was correlated with the iron sorbing capacity of the cells. Microscopic analysis showed that iron accumulated within ETY cells. Proteinase-treated ETY cells had no iron sorbing capacity. On the basis of these results, we conclude that intracellular proteins are involved in iron sorption by ETY cells.
authors.affiliation: Central Laboratories for Frontier Technology, KIRIN Holdings Co., Ltd., 1-13-5 Fukuura Kanazawa-ku, Yokohama-shi, Kanagawa 236-0004, Japan. Toshikazu_2_Tsuji@kirin.co.jp
authors.forename: Toshikazu
authors.initials: T
authors.lastname: Tsuji
authors.forename: Yuki
authors.initials: Y
authors.lastname: Konoeda
authors.forename: Keiko
authors.initials: K
authors.lastname: Kanai
authors.forename: Aki
authors.initials: A
authors.lastname: Yokoyama
authors.forename: Satoshi
authors.initials: S
authors.lastname: Yoshida
mesh_terms: D000327:Adsorption; D001692:Biological Transport / Q000187:drug effects; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D002463:Cell Membrane Permeability / Q000187:drug effects; D002473:Cell Wall / Q000187:drug effects / Q000378:metabolism; D000431:Ethanol / Q000378:metabolism / Q000494:pharmacology*; D005285:Fermentation; D006801:Humans; D007501:Iron / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism*; D014920:Wine / Q000032:analysis / Q000382:microbiology
chemical_list: D029701:Saccharomyces cerevisiae Proteins; D000431:Ethanol; D007501:Iron
keywords: Biosorption; Ethanol; Iron; Protein; Wine; Yeast