title: Phenotypic and functional characteristic of a newly identified CD8+ Foxp3- CD103+ regulatory T cells.
abstract: TGF-β and Foxp3 expressions are crucial for the induction and functional activity of CD4(+)Foxp3(+) regulatory T (iTreg) cells. Here, we demonstrate that although TGF-β-primed CD8(+) cells display much lower Foxp3 expression, their suppressive capacity is equivalent to that of CD4(+) iTreg cells, and both Foxp3(-) and Foxp3(+) CD8+ subsets have suppressive activities in vitro and in vivo. CD8(+)Foxp3(-) iTreg cells produce little IFN-γ but almost no IL-2, and display a typical anergic phenotype. Among phenotypic markers expressed in CD8(+)Foxp3(-) cells, we identify CD103 expression particularly crucial for the generation and function of this subset. Moreover, IL-10 and TGF-β signals rather than cytotoxicity mediate the suppressive effect of this novel Treg population. Therefore, TGF-β can induce both CD8(+)Foxp3(-) and CD8(+)Foxp3(+) iTreg subsets, which may represent the unique immunoregulatory means to treat autoimmune and inflammatory diseases.
authors.affiliation: Department of Nephrology, Sun Yat-sen Memorial Hospital of Sun Yat-sen University, Guangzhou 510120, China.
authors.forename: Ya
authors.initials: Y
authors.lastname: Liu
authors.forename: Qin
authors.initials: Q
authors.lastname: Lan
authors.forename: Ling
authors.initials: L
authors.lastname: Lu
authors.forename: Maogen
authors.initials: M
authors.lastname: Chen
authors.forename: Zanxian
authors.initials: Z
authors.lastname: Xia
authors.forename: Jilin
authors.initials: J
authors.lastname: Ma
authors.forename: Julie
authors.initials: J
authors.lastname: Wang
authors.forename: Huimin
authors.initials: H
authors.lastname: Fan
authors.forename: Yi
authors.initials: Y
authors.lastname: Shen
authors.forename: Bernhard
authors.initials: B
authors.lastname: Ryffel
authors.forename: David
authors.initials: D
authors.lastname: Brand
authors.forename: Francisco
authors.initials: F
authors.lastname: Quismorio
authors.forename: Zhongmin
authors.initials: Z
authors.lastname: Liu
authors.forename: David A
authors.initials: DA
authors.lastname: Horwitz
authors.forename: Anping
authors.initials: A
authors.lastname: Xu
authors.forename: Song Guo
authors.initials: SG
authors.lastname: Zheng
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000378:metabolism*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D002454:Cell Differentiation; D051858:Forkhead Transcription Factors / Q000378:metabolism*; D039001:Integrin alpha Chains / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D010641:Phenotype; D011948:Receptors, Antigen, T-Cell / Q000502:physiology; D015398:Signal Transduction; D050378:T-Lymphocytes, Regulatory / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism; D016212:Transforming Growth Factor beta / Q000494:pharmacology
chemical_list: D015703:Antigens, CD; C418974:FOXP3 protein, human; D051858:Forkhead Transcription Factors; D039001:Integrin alpha Chains; D011948:Receptors, Antigen, T-Cell; D016212:Transforming Growth Factor beta; C091367:alpha E integrins
keywords: CD103; CD8+; Foxp3; TGF-β; regulatory T cells