title: Analysis of the nonstructural and structural polyprotein regions, and complete genome sequences of Israel acute paralysis viruses identified from honeybees (Apis mellifera) in Korea.
abstract: Phylogenetic trees were constructed for 24 partial nucleotide sequences of the nonstructural polyprotein (ORF1) and structural polyprotein regions (ORF2) of Korean IAPV genotypes, as well as eight previously reported IAPV sequences from various countries. Most of the Korean genotypes formed a distinct cluster, separate from other country genotypes. To investigate this phenomenon in more detail, three complete IAPV genome sequences were identified from different regions in Korea, i.e., Korea1, Korea2, and Korea3. These sequences were aligned with eight previously reported complete genome sequences and various genome regions were compared. The Korean IAPVs were very similar to those from China and Israel, but highly diverged from USA and Australian genotypes. Interestingly, they showed greater variability than the USA and Australian genotypes in ORF1, but highly similar to the Australian genotype in the ORF2 region. Thus, genetic recombination may account for the spatial distance between the Korean IAPV genotypes and those from other countries.
authors.affiliation: Parasitology and Insect Disease Research Laboratory, Animal and Plant Quarantine Agency, 480 Anyang, 6 dong, Anyang 420-480, South Korea. eswar@mail.hallym.ac.kr
authors.forename: Kondreddy Eswar
authors.initials: KE
authors.lastname: Reddy
authors.forename: Jin Hyeong
authors.initials: JH
authors.lastname: Noh
authors.forename: Young-Ha
authors.initials: YH
authors.lastname: Kim
authors.forename: Mi Sun
authors.initials: MS
authors.lastname: Yoo
authors.forename: Huong Thi Thanh
authors.initials: HT
authors.lastname: Doan
authors.forename: Mummadireddy
authors.initials: M
authors.lastname: Ramya
authors.forename: Suk-Chan
authors.initials: SC
authors.lastname: Jung
authors.forename: Dong Van
authors.initials: DV
authors.lastname: Quyen
authors.forename: Seung-Won
authors.initials: SW
authors.lastname: Kang
mesh_terms: D000818:Animals; D001516:Bees / Q000821:virology*; D016000:Cluster Analysis; D056629:Dicistroviridae / Q000235:genetics* / Q000302:isolation & purification*; D016679:Genome, Viral; D005838:Genotype; D007723:Korea; D008969:Molecular Sequence Data; D058974:Phylogeography; D020815:Polyproteins / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics*; D011995:Recombination, Genetic; D017422:Sequence Analysis, DNA; D017361:Viral Nonstructural Proteins / Q000235:genetics; D015678:Viral Structural Proteins / Q000235:genetics
chemical_list: D020815:Polyproteins; D012367:RNA, Viral; D017361:Viral Nonstructural Proteins; D015678:Viral Structural Proteins
keywords: Apis mellifera; Honeybee; Israel acute paralysis virus; Phylogenetic tree