title: Expression of p21ras-related protein in the plasma and tissue of patients with adenomas and carcinomas of the colon.
abstract: Abstract Over-expression of p21 ras-related protein was determined in the plasma by immunoblotting and in the tissue by immuno-histochemistry in a cohort of patients undergoing colonoscopy. In the plasma samples, p21 ras over-expression was detected in: 9% (4/47) of normal controls; 21% (13/61) of individuals with normal colonoscopies but with a prior history of colonic neoplasia; 12% (4/33) of small adenoma patients, 29% (6/21) of large adenoma patients; 63% (5/8) of carcinoma-in-adenoma patients; 50% (2/4) of Dukes' A carcinoma patients; and 20% (2/10) of Dukes' B-D carcinoma patients. In the tissue samples, p21 ras over-expression was detected in: 25% (2/8) of small adenoma patients; 44% (4/9) of large adenoma patients; 100% (4/4) of carcinoma-in-adenoma patients; and 33% (1/3) of Dukes' B-C carcinoma patients. For matched plasma-tissue pairs, there was a statistically significant correlation for p21 ras over-expression (R = 0.47, p = 0.02).
authors.affiliation: the Division, of Environmental Health Sciences, Columbia University College of phvsicians and Surgeons and School of Public Health, 60 Haven Avenue, B-1, New York, NY, 10032, USA.
authors.forename: J C
authors.initials: JC
authors.lastname: Luo
authors.forename: A I
authors.initials: AI
authors.lastname: Neugut
authors.forename: G
authors.initials: G
authors.lastname: Garbowski
authors.forename: K A
authors.initials: KA
authors.lastname: Forde
authors.forename: M
authors.initials: M
authors.lastname: Treat
authors.forename: S
authors.initials: S
authors.lastname: Smith
authors.forename: H
authors.initials: H
authors.lastname: Niman
authors.forename: P W
authors.initials: PW
authors.lastname: Brandt-Rauf