title: HLA-G expression in the peripheral blood of live kidney transplant recipients.
abstract: BACKGROUND
  The human leukocyte antigen-G (HLA-G) has been considered to be an important tolerogeneic molecule playing an essential role in maternal-fetal tolerance, upregulated in the context of transplantation, malignancy, and inflammation, and has been correlated with various clinical outcomes. The aim of this study was to investigate the clinical relevance of the expression of membrane HLA-G (mHLA-G), intracellular HLA-G (iHLA-G), and soluble HLA-G (sHLA-G) in the peripheral blood of live kidney transplant recipients.
  
  
  METHODS
  We compared the expression of the three HLA-G isoforms in three groups, healthy donors (n=20), recipients with acute rejection (n=19), and functioning transplants (n=30). Flow cytometry was used to detect the expression of mHLA-G and iHLA-G in the T lymphocytes of peripheral blood from subjects in the three groups. Enzyme-linked immunosorbent assays were used to detect sHLA-G in the plasma from the three groups.
  
  
  RESULTS
  There were no significant differences in mHLA-G and intracellular HLA-G among the three groups, but the sHLA-G plasma level was higher in the functioning group than in the acute rejection or healthy group. We found a subset of CD4(+)HLA-G(+) and CD8(+)HLA-G(+) T lymphocytes with low rates of mHLA-G expression in the peripheral blood of kidney transplantation recipients. Intracellular expression of HLA-G was detected in T lymphocytes. However, there was no correlation between acute rejection and the mHLA-G or intracellular HLA-G expression.
  
  
  CONCLUSIONS
  sHLA-G was the major isoform in the peripheral blood of live kidney transplant recipients and high sHLA-G levels were associated with allograft acceptance.
authors.affiliation: 309th Hospital of the People's Liberation Army, Beijing, China.
authors.forename: Li
authors.initials: L
authors.lastname: Xiao
authors.forename: Wen-qiang
authors.initials: WQ
authors.lastname: Zhou
authors.forename: Bing-yi
authors.initials: BY
authors.lastname: Shi
authors.forename: Kai
authors.initials: K
authors.lastname: Feng
authors.forename: Xiu-yun
authors.initials: XY
authors.lastname: He
authors.forename: Yu-xiang
authors.initials: YX
authors.lastname: Wei
authors.forename: Yu
authors.initials: Y
authors.lastname: Gao
authors.forename: Xiao-yun
authors.initials: XY
authors.lastname: Meng
authors.forename: Wei
authors.initials: W
authors.lastname: Xiao
mesh_terms: D000328:Adult; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D059951:HLA-G Antigens / Q000097:blood*; D006801:Humans; D016030:Kidney Transplantation; D019520:Living Donors; D008297:Male; D008875:Middle Aged; D013601:T-Lymphocytes / Q000276:immunology
chemical_list: D059951:HLA-G Antigens