title: Improvement of manganese peroxidase production by the hyper lignin-degrading fungus Phanerochaete sordida YK-624 by recombinant expression of the 5-aminolevulinic acid synthase gene.
abstract: The manganese peroxidase (MnP) gene (mnp4) promoter of Phanerochaete sordida YK-624 was used to drive expression of 5-aminolevulinic acid synthase (als), which is a key heme biosynthesis enzyme. The expression plasmid pMnP4pro-als was transformed into P. sordida YK-624 uracil auxotrophic mutant UV-64, and 14 recombinant als expressing-transformants were generated. Average cumulative MnP activities in the transformants were 1.18-fold higher than that of control transformants. In particular, transformants A-14 and A-61 showed significantly higher MnP activity (approximately 2.8-fold) than wild type. RT-PCR analysis indicated that the increased MnP activity was caused by elevated recombinant als expression. These results suggest that the production of MnP is improved by high expression of als.
authors.affiliation: Graduate School of Agriculture, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka, 422-8529, Japan, ahhirai@ipc.shizuoka.ac.jp.
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Hirai
authors.forename: Kenta
authors.initials: K
authors.lastname: Misumi
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Suzuki
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Kawagishi
mesh_terms: D000624:5-Aminolevulinate Synthetase / Q000235:genetics* / Q000378:metabolism*; D020869:Gene Expression Profiling; D060847:Metabolic Engineering / Q000379:methods*; D010544:Peroxidases / Q000096:biosynthesis* / Q000235:genetics*; D020075:Phanerochaete / Q000201:enzymology* / Q000235:genetics* / Q000378:metabolism; D010957:Plasmids; D060888:Real-Time Polymerase Chain Reaction; D014170:Transformation, Genetic
chemical_list: D010544:Peroxidases; C051129:manganese peroxidase; D000624:5-Aminolevulinate Synthetase