title: Serial monoxenous transmission of Toxoplasma gondii in cats.
abstract: Abstract :  Oral administration of Toxoplasma gondii oocysts to cats (i.e., monoxenous transmission) typically induces patent infections in fewer than half of test subjects. In the present study, oral administration of T. gondii oocysts to 5 kittens induced a patent infection in 2 of them, but only 1 kitten shed enough oocysts to enable further study. Those monoxenously-produced oocysts were administered to another kitten, which produced a second generation of monoxenous oocysts, and then those were used to induce a third generation of monoxenous oocysts. These results provide a rationale to develop a strain of T. gondii that has efficient direct transmission. The isolate of T. gondii that was able to be passaged in this manner has been designated the Dubey strain and cultured tachyzoites have been donated to a repository.
authors.affiliation: Department of Pathobiology of the University of Illinois at Urbana-Champaign, Illinois 61802;
authors.forename: H O
authors.initials: HO
authors.lastname: Awobode
authors.forename: A J
authors.initials: AJ
authors.lastname: Paul
authors.forename: H
authors.initials: H
authors.lastname: Sengoku-Graham
authors.forename: R W
authors.initials: RW
authors.lastname: Clem
authors.forename: N E
authors.initials: NE
authors.lastname: Mateus-Pinilla
authors.forename: M M
authors.initials: MM
authors.lastname: McAllister
mesh_terms: D000818:Animals; D002371:Cat Diseases / Q000469:parasitology / Q000635:transmission*; D002415:Cats; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D033661:Oocysts / Q000502:physiology; D012692:Serial Passage / Q000379:methods / Q000662:veterinary; D013552:Swine; D014122:Toxoplasma / Q000502:physiology*; D014124:Toxoplasmosis, Animal / Q000469:parasitology / Q000635:transmission*