title: [Construction of keratinocyte growth factor phage active peptides for the promotion of epidermal cell proliferation].
abstract: OBJECTIVE
  To construct and display the keratinocyte growth factor (KGF) phage active peptides so as to detect the promoting effects of epidermal cell.
  
  
  METHODS
  KGF sequences were chosen and their primers were designed. The selected genes of P1, P2 and P4 were obtained by reverse transcription (RT)-PCR. P3 was obtained by direct synthesis. And the KGF genes were subcloned into pComb3 vector. The technique of phage display was employed to display the genes on phage surface. Methyl thiazolyl tetrazolium (MTT) assay was used to evaluate the promoting effects of KGF phage active peptides on the proliferation of epidermal cell. Optical density (A) was determined at 570 nm. Immunofluorescent assay was employed to evaluate the cell affinity of KGF phage active peptides.
  
  
  RESULTS
  The four KGF genes were obtained and subcloned into pComb3 vector. The proteins of the KGF genes were expressed on the surface of the pComb3 vector. The MTT data of optical density (A) showed that significant differences existed between the negative control and KGF control (0.293 ± 0.017 vs 0.520 ± 0.043) and KGF phage active peptide groups (0.293 ± 0.017 vs 0.469 ± 0.057, 0.441 ± 0.048, 0.438 ± 0.035, 0.446 ± 0.037) (all P < 0.01). The results of immunofluorescent assay indicated that KGF and KGF phage active peptides had excellent cell affinity.
  
  
  CONCLUSIONS
  KGF phage active peptides are successfully constructed and displayed and they may promote the proliferation of epidermal cell.
authors.affiliation: Sixteenth Department of Cosmetic Plastic Surgery, Chinese Academy of Medical Sciences, Beijing, China.
authors.forename: Xian-lei
authors.initials: XL
authors.lastname: Zong
authors.forename: Du-yin
authors.initials: DY
authors.lastname: Jiang
authors.forename: Guo-ju
authors.initials: GJ
authors.lastname: Li
authors.forename: Jing-long
authors.initials: JL
authors.lastname: Cai
mesh_terms: D001435:Bacteriophages / Q000378:metabolism*; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D000078404:Epidermal Cells; D004847:Epithelial Cells / Q000166:cytology; D051523:Fibroblast Growth Factor 7 / Q000235:genetics / Q000494:pharmacology*; D006801:Humans; D010455:Peptides / Q000494:pharmacology*
chemical_list: D010455:Peptides; D051523:Fibroblast Growth Factor 7