title: MicroRNA-187, down-regulated in clear cell renal cell carcinoma and associated with lower survival, inhibits cell growth and migration though targeting B7-H3.
abstract: Aberrantly expressed microRNAs (miRNAs) are frequently associated with the aggressive malignant behavior of human cancers, including clear cell renal cell carcinoma (ccRCC). Based on the preliminary deep sequencing data, we hypothesized that miR-187 may play an important role in ccRCC development. In this study, we found that miR-187 was down-regulated in both tumor tissue and plasma of ccRCC patients. Lower miR-187 expression levels were associated with higher tumor grade and stage. All patients with high miR-187 expression survived 5years, while with low miR-187 expression, only 42% survived. Suppressed in vitro proliferation, inhibited in vivo tumor growth, and decreased motility were observed in cells treated with the miR-187 expression vector. Further studies showed that B7 homolog 3 (B7-H3) is a direct target of miR-187. Over-expression of miR-187 decreased B7-H3 mRNA level and repressed B7-H3-3'-UTR reporter activity. Knockdown of B7-H3 using siRNA resulted in similar phenotype changes as that observed for overexpression of miR-187. Our data suggest that miR-187 is emerging as a novel player in the disease state of ccRCC. miR-187 plays a tumor suppressor role in ccRCC.
authors.affiliation: Foshan Maternal and Child Health Care Hospital, Foshan, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Zhao
authors.forename: Ting
authors.initials: T
authors.lastname: Lei
authors.forename: Congjie
authors.initials: C
authors.lastname: Xu
authors.forename: Huan
authors.initials: H
authors.lastname: Li
authors.forename: Wenmin
authors.initials: W
authors.lastname: Ma
authors.forename: Yunxia
authors.initials: Y
authors.lastname: Yang
authors.forename: Shuming
authors.initials: S
authors.lastname: Fan
authors.forename: Yuchen
authors.initials: Y
authors.lastname: Liu
mesh_terms: D020413:3' Untranslated Regions; D000818:Animals; D060887:B7 Antigens / Q000378:metabolism*; D002292:Carcinoma, Renal Cell / Q000378:metabolism*; D002465:Cell Movement; D049109:Cell Proliferation; D002470:Cell Survival; D015536:Down-Regulation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D016147:Genes, Tumor Suppressor; D006801:Humans; D007680:Kidney Neoplasms / Q000378:metabolism*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D035683:MicroRNAs / Q000378:metabolism*; D008875:Middle Aged; D009368:Neoplasm Transplantation; D011379:Prognosis; D012333:RNA, Messenger / Q000378:metabolism; D016896:Treatment Outcome
chemical_list: D020413:3' Untranslated Regions; D060887:B7 Antigens; C423237:CD276 protein, human; C571843:MIRN187 microRNA, human; D035683:MicroRNAs; D012333:RNA, Messenger
keywords: B7-H3; Clear cell renal cell carcinoma; Migration; Proliferation; Survival; miR-187