title: [Effect of autologous platelet-rich plasma on heart infarction in sheep].
abstract: OBJECTIVE
  Myocardial infarction is the most common cause of congestive heart failure. The objective of this work is to evaluate, in experimental animals, morphological and histological effects of the implantation of autologous platelet-rich plasma in infarcted heart sheep.
  
  
  METHODS
  Twenty-four ewes were used, they were surgically infarcted through left thoracotomy and two coronary arteries were ligated (first and second diagonal). After coronary artery ligation three sheep died of ventricular fibrillation. Three weeks after coronary ligation, sheep were reoperated through median sternotomy. Normal saline solution was injected in the infarcted zone in 6 of them (control group) whereas platelet gel was injected in 15 of them. All sheep were euthanized at 9 weeks of evolution of the second surgery.
  
  
  RESULTS
  Noteworthy is the formation of new vessels in hematoxylin-eosin-stained sections and factor viii in plasma rich in growth-factors (PRGF)-treated hearts.
  
  
  CONCLUSIONS
  Injection of platelet growth factors, PRGF, in previously infarcted sheep hearts promotes mitogenesis and angiogenesis. The use of autologous PRGF is simple and safe, causing no toxicity or immune-inflammatory reactions.
authors.affiliation: Servicio de Cirugía Cardiaca, Policlínica Gipuzkoa, San Sebastián, España.
authors.forename: Ignacio
authors.initials: I
authors.lastname: Gallo
authors.forename: Alberto
authors.initials: A
authors.lastname: Sáenz
authors.forename: Adolfo
authors.initials: A
authors.lastname: Arévalo
authors.forename: Sonia
authors.initials: S
authors.lastname: Roussel
authors.forename: Ignacio
authors.initials: I
authors.lastname: Pérez-Moreiras
authors.forename: Edurne
authors.initials: E
authors.lastname: Artiñano
authors.forename: Ana
authors.initials: A
authors.lastname: Martínez-Peñuela
authors.forename: Javier
authors.initials: J
authors.lastname: Esquide
authors.forename: Antonio
authors.initials: A
authors.lastname: Aspiroz
authors.forename: Ignacio
authors.initials: I
authors.lastname: Camacho
mesh_terms: D000818:Animals; D005260:Female; D009203:Myocardial Infarction / Q000473:pathology / Q000628:therapy*; D053657:Platelet-Rich Plasma; D012756:Sheep
keywords: Cardiac surgery; Cirugía cardíaca; Células madre; España; Infarto de miocardio; Myocardial infarction; Plaquetas; Platelets; Remodelación ventricular; Spain; Stem cells; Ventricular remodeling