title: Antiviral activity of angelicin against gammaherpesviruses.
abstract: Human gammaherpesviruses including Epstein-Barr virus (EBV) and Kaposi's sarcoma-associated herpesvirus (KSHV) are important pathogens as they persist in the host and cause various malignancies. However, few antiviral drugs are available to efficiently control gammaherpesvirus replication. Here we identified the antiviral activity of angelicin against murine gammaherpesvirus 68 (MHV-68), genetically and biologically related to human gammaherpesviruses. Angelicin, a furocoumarin naturally occurring tricyclic aromatic compound, efficiently inhibited lytic replication of MHV-68 in a dose-dependent manner following the virus entry. The IC50 of angelicin antiviral activity was estimated to be 28.95μM, while the CC50 of angelicin was higher than 2600μM. Furthermore, incubation with angelicin efficiently inhibited 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced lytic replication of human gammaherpresviruses in both EBV- and KSHV-infected cells. Taken together, these results suggest that MHV-68 can be a useful tool to screen novel antiviral agents against human gammaherepsviruses and that angelicin may provide a lead structure for the development of antiviral drug against gammaherpesviruses.
authors.affiliation: Department of Biosystems and Biotechnology, Division of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul 136-713, Republic of Korea.
authors.forename: Hye-Jeong
authors.initials: HJ
authors.lastname: Cho
authors.forename: Seon-Gyeong
authors.initials: SG
authors.lastname: Jeong
authors.forename: Ji-Eun
authors.initials: JE
authors.lastname: Park
authors.forename: Jin-Ah
authors.initials: JA
authors.lastname: Han
authors.forename: Hye-Ri
authors.initials: HR
authors.lastname: Kang
authors.forename: Dongho
authors.initials: D
authors.lastname: Lee
authors.forename: Moon Jung
authors.initials: MJ
authors.lastname: Song
mesh_terms: D000998:Antiviral Agents / Q000494:pharmacology*; D002460:Cell Line; D011564:Furocoumarins / Q000494:pharmacology*; D018142:Gammaherpesvirinae / Q000187:drug effects* / Q000235:genetics / Q000502:physiology; D006566:Herpesviridae Infections / Q000821:virology*; D006801:Humans; D017735:Virus Latency / Q000187:drug effects; D014779:Virus Replication / Q000187:drug effects
chemical_list: D000998:Antiviral Agents; D011564:Furocoumarins; C011659:angelicin
keywords: Angelicin; Antiviral activity; EBV; Furocoumarins; KSHV; MHV-68