title: Immunohistochemical approach reveals localization of cystathionine-γ-lyase and cystathionine-β-synthetase in ethanol-induced gastric mucosa damage in mice.
abstract: BACKGROUND
  Hydrogen sulphide (H2S) has been proved to be a neuromodulator and contributes to the maintenance of gastric mucosal integrity in damage caused by anti-inflammatory nonsteroidal drugs. Previously, we demonstrated that H2S synthesis is essential to gastric protection against ethanol.
  
  
  OBJECTIVE
  To better understanding the role of H2S and the detailed localization of its production in both normal and injured stomach due to ethanol injection, we studied the expression of cystathionine-γ-lyase (CSE) and cystathionine-β-synthetase (CBS) isoforms in gastric mucosa of mice treated with saline or 50% ethanol.
  
  
  METHODS
  Mice were treated by gavage with saline or 50% ethanol (0.5 mL/25 g). After 1 hour, mice were sacrificed, and gastric tissue was evaluated by histological and immunohistochemical analysis specific for CSE and CBS.
  
  
  RESULTS
  We have demonstrated a non-specific expression of CBS in the normal gastric mucosa and expression of CSE occurring mainly in the parietal cells of the animals treated with ethanol.
  
  
  CONCLUSIONS
  Thus, we demonstrated that the expression of CBS appears to be constitutive and diffuse across the gastric epithelium, while the expression of CSE appears to be induced in parietal cells by damage agents such as ethanol.
authors.affiliation: Biotechnology and Biodiversity Center Research (BIOTEC), Federal University of Piauí, Campus de Parnaíba, Parnaíba, PI, Brazil. jandvenes@ufpi.edu.br
authors.forename: Jand-Venes Rolim
authors.initials: JV
authors.lastname: Medeiros
authors.forename: Pedro Marcos Gomes
authors.initials: PM
authors.lastname: Soares
authors.forename: Gerly Anne de
authors.initials: GA
authors.lastname: Castro Brito
authors.forename: Marcellus Henrique Loiola Ponte de
authors.initials: MH
authors.lastname: Souza
mesh_terms: D000818:Animals; D003541:Cystathionine beta-Synthase / Q000378:metabolism*; D003542:Cystathionine gamma-Lyase / Q000378:metabolism*; D004195:Disease Models, Animal; D000431:Ethanol / Q000494:pharmacology; D005753:Gastric Mucosa / Q000187:drug effects / Q000201:enzymology* / Q000473:pathology; D006862:Hydrogen Sulfide / Q000378:metabolism*; D007150:Immunohistochemistry; D051379:Mice
chemical_list: D000431:Ethanol; D003541:Cystathionine beta-Synthase; D003542:Cystathionine gamma-Lyase; D006862:Hydrogen Sulfide