title: Reduced expression of steroid sulfatase in primary colorectal cancer.
abstract: Colorectal cancer (CRC) is considered an estrogen-dependent malignancy, and intratissue estrogen concentration can be controlled by steroid sulfatase (STS). Little is known about changes in the expression of STS during the development of CRC. Therefore, we analysed the STS mRNA levels in primary colonic adenocarcinoma tissues and adjacent histopathologically unchanged colonic mucosa from patients who underwent radical colon resection (n=90). We found a statistically significant decrease in STS transcript levels in CRC (P=0.0453). Moreover, we found that sodium butyrate (NaBu) significantly upregulated STS transcript levels in DLD-1 and HCT116 CRC cells. Our results suggest that STS expression can be decreased in the process of large intestinal carcinogenesis. Moreover, we observed that NaBu might increase STS expression in CRC cells.
authors.affiliation: Department of Biochemistry and Molecular Biology, Poznań University of Medical Sciences, Poznań, Poland.
authors.forename: Agnieszka Anna
authors.initials: AA
authors.lastname: Rawłuszko
authors.forename: Monica
authors.initials: M
authors.lastname: Antoniucci
authors.forename: Karolina
authors.initials: K
authors.lastname: Horbacka
authors.forename: Margarita
authors.initials: M
authors.lastname: Lianeri
authors.forename: Piotr
authors.initials: P
authors.lastname: Krokowicz
authors.forename: Paweł Piotr
authors.initials: PP
authors.lastname: Jagodziński
mesh_terms: D000230:Adenocarcinoma / Q000201:enzymology*; D000368:Aged; D020148:Butyric Acid / Q000494:pharmacology; D045744:Cell Line, Tumor; D003106:Colon / Q000201:enzymology; D015179:Colorectal Neoplasms / Q000201:enzymology*; D005260:Female; D006801:Humans; D007413:Intestinal Mucosa / Q000201:enzymology; D008297:Male; D043266:Steryl-Sulfatase / Q000096:biosynthesis*; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D020148:Butyric Acid; D043266:Steryl-Sulfatase
keywords: Butyrate; Colorectal cancer; Steroid sulfatase