title: Autonomous molecular cascades for evaluation of cell surfaces.
abstract: Molecular automata are mixtures of molecules that undergo precisely defined structural changes in response to sequential interactions with inputs. Previously studied nucleic acid-based automata include game-playing molecular devices (MAYA automata) and finite-state automata for the analysis of nucleic acids, with the latter inspiring circuits for the analysis of RNA species inside cells. Here, we describe automata based on strand-displacement cascades directed by antibodies that can analyse cells by using their surface markers as inputs. The final output of a molecular automaton that successfully completes its analysis is the presence of a unique molecular tag on the cell surface of a specific subpopulation of lymphocytes within human blood cells.
authors.affiliation: Research Division, Hospital for Special Surgery, 535E 70th Street, New York 10021, New York, USA.
authors.forename: Maria
authors.initials: M
authors.lastname: Rudchenko
authors.forename: Steven
authors.initials: S
authors.lastname: Taylor
authors.forename: Payal
authors.initials: P
authors.lastname: Pallavi
authors.forename: Alesia
authors.initials: A
authors.lastname: Dechkovskaia
authors.forename: Safana
authors.initials: S
authors.lastname: Khan
authors.forename: Vincent P
authors.initials: VP
authors.lastname: Butler
authors.forename: Sergei
authors.initials: S
authors.lastname: Rudchenko
authors.forename: Milan N
authors.initials: MN
authors.lastname: Stojanovic
mesh_terms: D000906:Antibodies / Q000378:metabolism; D001773:Blood Cells / Q000737:chemistry*; D002454:Cell Differentiation / Q000502:physiology; D019070:Cell Lineage / Q000502:physiology; D061505:Cell Surface Display Techniques; D039301:Computers, Molecular; D006801:Humans; D019809:Streptavidin / Q000378:metabolism; D013601:T-Lymphocytes / Q000378:metabolism
chemical_list: D000906:Antibodies; D019809:Streptavidin
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