title: Genotyping of Toxoplasma gondii Isolates from Soil Samples in Tehran, Iran.
abstract: BACKGROUND
  The protozoan parasite Toxoplasma gondii can infect any warm blooded nucleated cells. One of the ways for human infection is ingestion of oocysts directly from soil or via infected fruits or vegetables. To survey the potential role of T. gondii oocyst in soil samples, the present study was conducted in Tehran City, Iran.
  
  
  METHODS
  A total of 150 soil samples were collected around rubbish dumps, children's play ground, parks and public places. Oocysts recovery was performed by sodium nitrate flotation method on soil samples. For molecular detection, PCR reaction targeting B1 gene was performed and then, the positive results were confirmed using repetitive 529 bp DNA fragment in other PCR reaction. Finally, the positive samples were genotyped at the SAG2 locus.
  
  
  RESULTS
  Toxoplasma DNA was found in 13 soil samples. After genotyping and RFLP analysis in SAG2 locus, nine positive samples were revealed type III, one positive sample was type I whereas three samples revealed mixed infection (type, I & III).
  
  
  CONCLUSIONS
  The predominant genotype in Tehran soil samples is type III.
authors.affiliation: Dept. of Medical Parasitology, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran ; Dept. of Parasitology and Mycology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: M
authors.initials: M
authors.lastname: Tavalla
authors.forename: H
authors.initials: H
authors.lastname: Oormazdi
authors.forename: L
authors.initials: L
authors.lastname: Akhlaghi
authors.forename: S
authors.initials: S
authors.lastname: Shojaee
authors.forename: E
authors.initials: E
authors.lastname: Razmjou
authors.forename: R
authors.initials: R
authors.lastname: Hadighi
authors.forename: Ar
authors.initials: A
authors.lastname: Meamar
keywords: Genotype; Iran; SAG2; Soil; Toxoplasma gondii
references.citation: J Trop Med Hyg. 1973 Jul;76(7):158-60
references.pmid: 4580065
references.citation: Int J Parasitol. 2008 Jul;38(8-9):1017-23
references.pmid: 18325523
references.citation: FEMS Microbiol Rev. 2003 Dec;27(5):651-61
references.pmid: 14638417
references.citation: Int J Parasitol. 2002 Jan;32(1):99-105
references.pmid: 11796127
references.citation: J Infect Dis. 1995 Dec;172(6):1561-6
references.pmid: 7594717
references.citation: Int J Parasitol. 2006 Jun;36(7):841-8
references.pmid: 16643922
references.citation: Parasitol Res. 2007 Jun;101(1):99-104
references.pmid: 17297630
references.citation: Int J Parasitol. 2003 Jan;33(1):97-103
references.pmid: 12547351
references.citation: J Clin Microbiol. 1997 Jun;35(6):1411-4
references.pmid: 9163454
references.citation: Int J Parasitol. 2002 Aug;32(9):1193-9
references.pmid: 12117502
references.citation: Wiad Parazytol. 2005;51(1):21-2
references.pmid: 16841685
references.citation: Vet Parasitol. 2003 May 30;114(2):89-95
references.pmid: 12781471
references.citation: Bull Soc Pathol Exot. 1997;90(1):19-21
references.pmid: 9264742
references.citation: J Eukaryot Microbiol. 2008 Nov-Dec;55(6):467-75
references.pmid: 19120791
references.citation: Vet Parasitol. 2011 Dec 29;183(1-2):59-67
references.pmid: 21764217
references.citation: Parasitology. 1995 Aug;111 ( Pt 2):127-32
references.pmid: 7675527
references.citation: Parasitol Res. 2007 Jun;101(1):111-5
references.pmid: 17333278
references.citation: Nature. 1992 Sep 3;359(6390):82-5
references.pmid: 1355855
references.citation: J Parasitol. 1973 Jun;59(3):587-8
references.pmid: 4711675
references.citation: Parasitol Res. 1995;81(1):32-7
references.pmid: 7724511
references.citation: J Clin Microbiol. 2006 Mar;44(3):720-4
references.pmid: 16517845
references.citation: Appl Environ Microbiol. 2003 Oct;69(10):5819-25
references.pmid: 14532030
references.citation: Int J Parasitol. 2009 Jul 1;39(8):895-901
references.pmid: 19217908
references.citation: J Parasitol. 1992 Oct;78(5):786-94
references.pmid: 1403418
references.citation: J Parasitol. 2011 Feb;97(1):40-3
references.pmid: 21348604
references.citation: Int J Parasitol. 2000 Jan;30(1):69-75
references.pmid: 10675747
references.citation: Zoonoses Public Health. 2010 Feb;57(1):34-52
references.pmid: 19744306
references.citation: Clin Microbiol Rev. 2012 Apr;25(2):264-96
references.pmid: 22491772
references.citation: Eur J Clin Microbiol Infect Dis. 2009 Jun;28(6):599-605
references.pmid: 19104853