title: Comparison of the antibacterial activity of an ozonated oil with chlorhexidine digluconate and povidone-iodine. A disk diffusion test.
abstract: Ozonated oils are antiseptics obtained from the chemical reaction between ozone and unsaturated fatty acids of vegetable oils. The aim of this study was to investigate the antimicrobial effectiveness of a commercially available ozonated oil (O3-Oil), in comparison with 0.2% chlorhexidine digluconate (CHX) and 10% povidone-iodine (PVP-I) through a disk diffusion test. For each antiseptic a series of two-fold dilutions was made, obtaining seven dilutions: 1:2, 1:4, 1:8, 1:16, 1:32, 1:64 and 1:128. The undiluted antiseptics and the seven dilutions were tested against two freeze-dried bacterial strains: Staphylococcus aureus (Sa) and Porphyromonas gingivalis (Pg). O3-Oil showed significantly greater diameters of growth inhibition (p<0.01) than CHX and PVP-I in all dilutions for both tested strains. CHX lost any antibacterial efficacy when diluted more than 1:32. At the highest dilution, the diameters of growth inhibition against Sa were 20.67±0.58 mm and 15.33±0.58 mm, for O3-Oil and PVP-I, respectively. At the same dilution, the diameters of growth inhibition against Pg were: 19.00 mm for O3-Oil and 13.67±0.58 mm for PVP-I. The promising results obtained for the O3-Oil, against the opportunistic Sa, and Pg, one of the main periodontal pathogens, suggest its potential applicability for periodontal treatment. Further preclinical and clinical investigations are warranted.
authors.affiliation: Department of Periodontology and Implantology, School of Dentistry, Alma Mater Studiorum.
authors.forename: Marco
authors.initials: M
authors.lastname: Montevecchi
authors.forename: Antonio
authors.initials: A
authors.lastname: Dorigo
authors.forename: Monica
authors.initials: M
authors.lastname: Cricca
authors.forename: Luigi
authors.initials: L
authors.lastname: Checchi
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D000891:Anti-Infective Agents, Local / Q000494:pharmacology*; D002710:Chlorhexidine / Q000031:analogs & derivatives* / Q000494:pharmacology; D052978:Disk Diffusion Antimicrobial Tests; D004353:Drug Evaluation, Preclinical; D005231:Fatty Acids, Unsaturated / Q000737:chemistry*; D010126:Ozone / Q000737:chemistry*; D010938:Plant Oils / Q000737:chemistry; D016966:Porphyromonas gingivalis / Q000187:drug effects / Q000254:growth & development; D011206:Povidone-Iodine / Q000494:pharmacology*; D013211:Staphylococcus aureus / Q000187:drug effects / Q000254:growth & development
chemical_list: D000900:Anti-Bacterial Agents; D000891:Anti-Infective Agents, Local; D005231:Fatty Acids, Unsaturated; D010938:Plant Oils; D010126:Ozone; D011206:Povidone-Iodine; C010882:chlorhexidine gluconate; D002710:Chlorhexidine