title: Internal disulfide bond acts as a switch for intein activity.
abstract: Inteins are intervening polypeptides that catalyze their own removal from flanking exteins, concomitant to the ligation of the exteins. The intein that interrupts the DP2 (large) subunit of DNA polymerase II from Methanoculleus marisnigri (Mma) can promote protein splicing. However, protein splicing can be prevented or reduced by overexpression under nonreducing conditions because of the formation of a disulfide bond between two internal intein Cys residues. This redox sensitivity leads to differential activity in different strains of E. coli as well as in different cell compartments. The redox-dependent control of in vivo protein splicing in an intein derived from an anaerobe that can occupy multiple environments hints at a possible physiological role for protein splicing.
authors.affiliation: Department of Chemistry, College of the Holy Cross, Worcester, Massachusetts 01610, United States.
authors.forename: Michael C
authors.initials: MC
authors.lastname: Nicastri
authors.forename: Kristina
authors.initials: K
authors.lastname: Xega
authors.forename: Lingyun
authors.initials: L
authors.lastname: Li
authors.forename: Jian
authors.initials: J
authors.lastname: Xie
authors.forename: Chunyu
authors.initials: C
authors.lastname: Wang
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Linhardt
authors.forename: Julie N
authors.initials: JN
authors.lastname: Reitter
authors.forename: Kenneth V
authors.initials: KV
authors.lastname: Mills
mesh_terms: D003545:Cysteine / Q000737:chemistry; D004257:DNA Polymerase II / Q000235:genetics; D004220:Disulfides / Q000494:pharmacology*; D004591:Electrophoresis, Polyacrylamide Gel; D047669:Exteins / Q000235:genetics; D047668:Inteins / Q000235:genetics*; D010084:Oxidation-Reduction; D019154:Protein Splicing / Q000187:drug effects / Q000235:genetics*; D053719:Tandem Mass Spectrometry
chemical_list: D004220:Disulfides; D004257:DNA Polymerase II; D003545:Cysteine
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