title: Structure of the caspase-recruitment domain from a zebrafish guanylate-binding protein.
abstract: The caspase-recruitment domain (CARD) mediates homotypic protein-protein interactions that assemble large oligomeric signaling complexes such as the inflammasomes during innate immune responses. Structural studies of the mammalian CARDs demonstrate that their six-helix bundle folds belong to the death-domain superfamily, whereas such studies have not been reported for other organisms. Here, the zebrafish interferon-induced guanylate-binding protein 1 (zIGBP1) was identified that contains an N-terminal GTPase domain and a helical domain typical of the mammalian guanylate-binding proteins, followed by a FIIND domain and a C-terminal CARD similar to the mammalian inflammasome proteins NLRP1 and CARD8. The structure of the zIGBP1 CARD as a fusion with maltose-binding protein was determined at 1.47 Å resolution. This revealed a six-helix bundle fold similar to the NLRP1 CARD structure with the bent α1 helix typical of all known CARD structures. The zIGBP1 CARD surface contains a positively charged patch near its α1 and α4 helices and a negatively charged patch near its α2, α3 and α5 helices, which may mediate its interaction with partner domains. Further studies using binding assays and other analyses will be required in order to address the physiological function(s) of this zebrafish protein.
authors.affiliation: Structural Immunobiology Unit, Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 4 Memorial Drive, Building 4, Room 228, Bethesda, MD 20892-0430, USA.
authors.forename: Tengchuan
authors.initials: T
authors.lastname: Jin
authors.forename: Mo
authors.initials: M
authors.lastname: Huang
authors.forename: Patrick
authors.initials: P
authors.lastname: Smith
authors.forename: Jiansheng
authors.initials: J
authors.lastname: Jiang
authors.forename: T Sam
authors.initials: TS
authors.lastname: Xiao
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D053468:CARD Signaling Adaptor Proteins / Q000737:chemistry* / Q000235:genetics*; D018360:Crystallography, X-Ray; D019204:GTP-Binding Proteins / Q000737:chemistry* / Q000235:genetics*; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000737:chemistry* / Q000235:genetics*; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D015027:Zebrafish; D029961:Zebrafish Proteins / Q000737:chemistry* / Q000235:genetics*
chemical_list: D053468:CARD Signaling Adaptor Proteins; C455623:CARD8 protein, human; C490287:GBP1 protein, human; D009363:Neoplasm Proteins; D029961:Zebrafish Proteins; D019204:GTP-Binding Proteins
keywords: CARDs; guanylate-binding proteins; six-helix bundle; zebrafish
references.citation: Trends Biochem Sci. 2011 Oct;36(10):541-52
references.pmid: 21798745
references.citation: Protein Sci. 2008 Oct;17(10):1771-80
references.pmid: 18715992
references.citation: Science. 2011 Oct 21;334(6054):376-80
references.pmid: 22021857
references.citation: PLoS One. 2011;6(11):e27396
references.pmid: 22087307
references.citation: PLoS One. 2012;7(8):e42775
references.pmid: 22880103
references.citation: Methods Enzymol. 1997;276:307-26
references.pmid: 27754618
references.citation: J Biol Chem. 1983 Jun 25;258(12):7746-50
references.pmid: 6305951
references.citation: Protein Sci. 2010 May;19(5):901-13
references.pmid: 20196072
references.citation: Acta Crystallogr D Biol Crystallogr. 2010 Feb;66(Pt 2):125-32
references.pmid: 20124692
references.citation: Biochem Biophys Res Commun. 2007 Feb 2;353(1):1-5
references.pmid: 17173864
references.citation: Curr Opin Struct Biol. 2009 Aug;19(4):449-57
references.pmid: 19477632
references.citation: Nature. 1999 Jun 10;399(6736):549-57
references.pmid: 10376594
references.citation: Cell. 2000 Sep 29;103(1):99-111
references.pmid: 11051551
references.citation: Proteins. 2013 Jul;81(7):1266-70
references.pmid: 23508996
references.citation: Cell. 2007 Feb 9;128(3):533-46
references.pmid: 17289572
references.citation: Nucleic Acids Res. 2010 Jul;38(Web Server issue):W545-9
references.pmid: 20457744
references.citation: Mol Cell. 2005 Dec 22;20(6):939-49
references.pmid: 16364918
references.citation: FEBS J. 2005 Oct;272(20):5101-9
references.pmid: 16218944
references.citation: Acta Crystallogr D Biol Crystallogr. 2010 Jan;66(Pt 1):12-21
references.pmid: 20057044
references.citation: Cell. 2010 Apr 30;141(3):446-57
references.pmid: 20434985
references.citation: Trends Immunol. 2004 Nov;25(11):601-9
references.pmid: 15489189
references.citation: Genome Biol. 2007;8(11):R251
references.pmid: 18039395
references.citation: Annu Rev Immunol. 2007;25:561-86
references.pmid: 17201679
references.citation: BMC Struct Biol. 2008 Feb 28;8:11
references.pmid: 18307765
references.citation: Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 May 1;69(Pt 5):482-7
references.pmid: 23695559
references.citation: Curr Opin Struct Biol. 2012 Apr;22(2):241-7
references.pmid: 22429337
references.citation: Nat Rev Mol Cell Biol. 2003 Feb;4(2):95-104
references.pmid: 12563287
references.citation: Nature. 2010 Jun 17;465(7300):885-90
references.pmid: 20485341
references.citation: Acta Crystallogr D Biol Crystallogr. 2010 Apr;66(Pt 4):486-501
references.pmid: 20383002
references.citation: BMC Evol Biol. 2008 Feb 06;8:42
references.pmid: 18254971
references.citation: Science. 2012 Apr 27;336(6080):481-5
references.pmid: 22461501
references.citation: Cell Host Microbe. 2012 Oct 18;12(4):432-44
references.pmid: 23084913
references.citation: Mol Cell. 2002 Aug;10(2):417-26
references.pmid: 12191486
references.citation: Immunity. 2012 Apr 20;36(4):561-71
references.pmid: 22483801
references.citation: Cell. 1998 Jul 24;94(2):171-80
references.pmid: 9695946
references.citation: Acta Crystallogr D Biol Crystallogr. 2013 May;69(Pt 5):774-84
references.pmid: 23633586
references.citation: J Appl Crystallogr. 2007 Aug 1;40(Pt 4):658-674
references.pmid: 19461840
references.citation: Trends Biochem Sci. 1997 May;22(5):155-6
references.pmid: 9175472
references.citation: Science. 1995 May 26;268(5214):1144-9
references.pmid: 7761829
references.citation: FEBS Lett. 2001 Mar 16;492(3):171-6
references.pmid: 11257489