title: Interactions between peptidyl tRNA hydrolase homologs and the ribosomal release factor Mrf1 in S. pombe mitochondria.
abstract: Mitochondrial translation synthesizes key subunits of the respiratory complexes. In Schizosaccharomyces pombe, strains lacking Mrf1, the mitochondrial stop codon recognition factor, are viable, suggesting that other factors can play a role in translation termination. S. pombe contains four predicted peptidyl tRNA hydrolases, two of which (Pth3 and Pth4), have a GGQ motif that is conserved in class I release factors. We show that high dosage of Pth4 can compensate for the absence of Mrf1 and loss of Pth4 exacerbates the lack of Mrf1. Also Pth4 is a component of the mitochondrial ribosome, suggesting that it could help recycling stalled ribosomes.
authors.affiliation: Centre de Génétique Moléculaire, UPR3404, FRC3115, Avenue de la Terrasse, 91198 Gif-sur-Yvette Cedex, France.
authors.forename: Laurent
authors.initials: L
authors.lastname: Dujeancourt
authors.forename: Ricarda
authors.initials: R
authors.lastname: Richter
authors.forename: Zofia M
authors.initials: ZM
authors.lastname: Chrzanowska-Lightowlers
authors.forename: Nathalie
authors.initials: N
authors.lastname: Bonnefoy
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Herbert
mesh_terms: D000595:Amino Acid Sequence; D002265:Carboxylic Ester Hydrolases / Q000378:metabolism*; D017353:Gene Deletion; D015151:Immunoblotting; D008928:Mitochondria / Q000378:metabolism*; D008969:Molecular Sequence Data; D012568:Schizosaccharomyces / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D029702:Schizosaccharomyces pombe Proteins / Q000737:chemistry / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D013728:Terminator Regions, Genetic
chemical_list: D029702:Schizosaccharomyces pombe Proteins; D002265:Carboxylic Ester Hydrolases; C019674:aminoacyl-tRNA hydrolase
keywords: Fission yeast; Mitochondria; Mitochondrial ribosome tagging; Peptidyl tRNA hydrolase; Release factor; Translation
references.citation: Science. 2008 Nov 7;322(5903):953-6
references.pmid: 18988853
references.citation: Biochimie. 1971;53(2):225-31
references.pmid: 4997677
references.citation: Nucleic Acids Res. 1990 Nov 25;18(22):6485-9
references.pmid: 2251111
references.citation: Nucleic Acids Res. 2008 Oct;36(18):5787-99
references.pmid: 18782833
references.citation: Arch Biochem Biophys. 2003 Dec 1;420(1):130-41
references.pmid: 14622983
references.citation: Mol Microbiol. 2012 Oct;86(1):37-50
references.pmid: 22857598
references.citation: Proc Natl Acad Sci U S A. 2002 Dec 24;99(26):16707-12
references.pmid: 12475929
references.citation: Microbiol Mol Biol Rev. 2000 Jun;64(2):281-315
references.pmid: 10839818
references.citation: Mol Gen Genet. 1996 May 23;251(2):204-10
references.pmid: 8668131
references.citation: EMBO J. 2010 Mar 17;29(6):1116-25
references.pmid: 20186120
references.citation: Yeast. 1994 Dec;10(13):1793-808
references.pmid: 7747518
references.citation: Ann Med. 2012 Feb;44(1):41-59
references.pmid: 21806499
references.citation: Eur J Biochem. 1970 Nov;16(3):430-8
references.pmid: 5477288
references.citation: Proteins. 2012 Nov;80(11):2629-42
references.pmid: 22821833
references.citation: Methods Mol Biol. 2008;457:113-24
references.pmid: 19066022
references.citation: Biotechniques. 2004 Feb;36(2):218-20
references.pmid: 14989084
references.citation: J Mol Biol. 2008 Apr 4;377(4):1024-37
references.pmid: 18304578
references.citation: Yeast. 2004 Nov;21(15):1289-305
references.pmid: 15546162
references.citation: Gene. 1987;57(2-3):267-72
references.pmid: 3319781
references.citation: Methods Mol Biol. 2008;432:65-81
references.pmid: 18370011
references.citation: Methods Mol Biol. 2007;372:91-105
references.pmid: 18314720
references.citation: Genetics. 1992 Jun;131(2):255-60
references.pmid: 1644270
references.citation: Yeast. 1996 Mar 15;12(3):259-65
references.pmid: 8904338
references.citation: Am J Hum Genet. 2010 Jul 9;87(1):115-22
references.pmid: 20598281
references.citation: Nucleic Acids Res. 2011 Oct;39(18):8029-41
references.pmid: 21727087
references.citation: Biochim Biophys Acta. 2013 Feb;1833(2):286-94
references.pmid: 22450032
references.citation: Mol Microbiol. 2011 May;80(3):772-85
references.pmid: 21418110
references.citation: Nucleic Acids Res. 1992 Sep 11;20(17):4423-8
references.pmid: 1408743
references.citation: Nucleic Acids Res. 2011 Mar;39(6):2221-33
references.pmid: 21076151
references.citation: Annu Rev Microbiol. 2008;62:353-73
references.pmid: 18544041
references.citation: Nucleic Acids Res. 2004 Nov 16;32(20):6028-37
references.pmid: 15547251
references.citation: Science. 2010 Jan 15;327(5963):301
references.pmid: 20075246
references.citation: Mol Cell. 2007 Sep 7;27(5):745-57
references.pmid: 17803939
references.citation: Antonie Van Leeuwenhoek. 1964;30:442-54
references.pmid: 14274138
references.citation: Proc Natl Acad Sci U S A. 1996 May 28;93(11):5253-7
references.pmid: 8643562
references.citation: Genetics. 2005 Apr;169(4):1891-901
references.pmid: 15695360
references.citation: Mol Gen Genet. 1983;191(1):91-8
references.pmid: 6350830
references.citation: Nature. 2008 Aug 14;454(7206):852-7
references.pmid: 18596689
references.citation: J Biol Chem. 1994 Jul 22;269(29):18899-903
references.pmid: 8034646
references.citation: FEMS Yeast Res. 2006 Sep;6(6):869-82
references.pmid: 16911509
references.citation: Methods Enzymol. 2002;350:445-69
references.pmid: 12073329
references.citation: Mol Gen Genet. 1988 Dec;215(1):81-6
references.pmid: 3241624
references.citation: Biochim Biophys Acta. 2012 Sep-Oct;1819(9-10):930-8
references.pmid: 22353467
references.citation: Mol Biol Evol. 2012 Nov;29(11):3497-512
references.pmid: 22688947
references.citation: Nat Biotechnol. 2006 Jul;24(7):841-7
references.pmid: 16823372
references.citation: Biol Direct. 2012 May 08;7:14
references.pmid: 22569235
references.citation: EMBO J. 1995 Jul 17;14(14):3445-51
references.pmid: 7628445
references.citation: Yeast. 2000 Jul;16(10):889-96
references.pmid: 10870100
references.citation: Mol Microbiol. 2000 Mar;35(5):1135-45
references.pmid: 10712694
references.citation: Yeast. 2001 Aug;18(11):1015-21
references.pmid: 11481672
references.citation: Anal Bioanal Chem. 2009 May;394(1):13-6
references.pmid: 19242681