title: [Clonal relatedness of bla(OXA-58)--carrying Acinetobacter baumannii clinical isolates].
abstract: OBJECTIVE
  To investigate the clonal relatedness of local bla(OXA-58)-carrying Acinetobacter baumannii clinical isolates.
  
  
  METHODS
  Non-duplicated isolates of Acinetobacter baumannii were collected in West China Hospital and verified by recA sequencing. Acquired bla(OXA-58) gene and natural bla(OXA51/66) genes were detected by PCR. Strain typing for bla(OXA-58)-carrying Acinetobacter baumannii isolates was performed by Enterobacterial repetitive intergenic consensus polymerase chain reaction (ERIC-PCR), multilocus sequence typing (MLST) and pulsed-field gel electrophoresis (PFGE).
  
  
  RESULTS
  A total of 115 Acinetobacter baumannii isolates were verified by recA gene and bla(OXA-51/6)6 detection. Among them, nine (7.8%) isolates carry bla(OXA-58) with reduced susceptibility to imipenem (MIC > or = 2 mg/L) were observed. ERIC-PCR fingerprints of nine bla(OXA-58)-carrying isolates were highly similar. MLST revealed that eight isolates were ST95 and one isolate was ST75. PFGE showed that eight isolates with the same sequence type were of the same fingerprint types, which were of two closely-related subtypes.
  
  
  CONCLUSIONS
  In West China Hospital, some Acinetobacter baumannii isolates with reduced susceptibility to carbapenem carried bla(OXA-58). The major spread way of bla(OXA-58)-carrying isolates was clonal dissemination.
authors.affiliation: Center of Infectious Diseases, West China Hospital, Sichuan University, Chengdu 610041, China.
authors.forename: Xiao-Hui
authors.initials: XH
authors.lastname: Wang
authors.forename: Zhi-Yong
authors.initials: ZY
authors.lastname: Zong
authors.forename: Xiao-Ju
authors.initials: XJ
authors.lastname: Lü
mesh_terms: D000151:Acinetobacter Infections / Q000382:microbiology; D040981:Acinetobacter baumannii / Q000201:enzymology* / Q000235:genetics* / Q000302:isolation & purification; D015780:Carbapenems / Q000494:pharmacology*; D003001:Cloning, Molecular; D024881:Drug Resistance, Bacterial; D015964:Gene Expression Regulation, Bacterial; D006801:Humans; D015378:Imipenem / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D001618:beta-Lactamases / Q000235:genetics*
chemical_list: D015780:Carbapenems; D015378:Imipenem; D001618:beta-Lactamases; C534889:beta-lactamase OXA-58, Acinetobacter baumannii