title: Insight into the family of Na+/Ca2+ exchangers of Caenorhabditis elegans.
abstract: Here we provide the first genome-wide in vivo analysis of the Na+/Ca2+ exchanger family in the model system Caenorhabditis elegans. We source all members of this family within the Caenorhabditis genus and reconstruct their phylogeny across humans and Drosophila melanogaster. Next, we provide a description of the expression pattern for each exchanger gene in C. elegans, revealing a wide expression in a number of tissues and cell types including sensory neurons, interneurons, motor neurons, muscle cells, and intestinal tissue. Finally, we conduct a series of behavioral and functional analyses through mutant characterization in C. elegans. From these data we demonstrate that, similar to mammalian systems, the expression of Na+/Ca2+ exchangers in C. elegans is skewed toward excitable cells, and we propose that C. elegans may be an ideal model system for the study of Na+/Ca2+ exchangers.
authors.affiliation: Department of Biological Sciences, The George Washington University, Washington, DC 20052.
authors.forename: Vishal
authors.initials: V
authors.lastname: Sharma
authors.forename: Chao
authors.initials: C
authors.lastname: He
authors.forename: Julian
authors.initials: J
authors.lastname: Sacca-Schaeffer
authors.forename: Eric
authors.initials: E
authors.lastname: Brzozowski
authors.forename: Daniel E
authors.initials: DE
authors.lastname: Martin-Herranz
authors.forename: Zelda
authors.initials: Z
authors.lastname: Mendelowitz
authors.forename: David A
authors.initials: DA
authors.lastname: Fitzpatrick
authors.forename: Damien M
authors.initials: DM
authors.lastname: O'Halloran
mesh_terms: D000818:Animals; D017173:Caenorhabditis elegans / Q000235:genetics*; D004331:Drosophila melanogaster / Q000235:genetics; D018507:Gene Expression Regulation, Developmental; D016678:Genome; D006801:Humans; D005810:Multigene Family / Q000235:genetics*; D010802:Phylogeny; D019831:Sodium-Calcium Exchanger / Q000235:genetics*; D014018:Tissue Distribution / Q000235:genetics
chemical_list: D019831:Sodium-Calcium Exchanger
keywords: Caenorhabditis elegans; comparative phylogeny; exchanger proteins; gene expression pattern; sodium calcium exchanger; sodium calcium potassium exchanger
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