title: Reduction of photosynthetic sensitivity in response to abiotic stress in tomato is mediated by a new generation plant activator.
abstract: BACKGROUND
  Yield losses as a result of abiotic stress factors present a significant challenge for the future of global food production. While breeding technologies provide potential to combat negative stress-mediated outcomes over time, interventions which act to prime plant tolerance to stress, via the use of phytohormone-based elicitors for example, could act as a valuable tool for crop protection. However, the translation of fundamental biology into functioning solution is often constrained by knowledge-gaps.
  
  
  RESULTS
  Photosynthetic and transcriptomic responses were characterised in young tomato (Solanum lycopersicum L.) seedlings in response to pre-treatment with a new plant health activator technology, 'Alethea', followed by a subsequent 100 mM salinity stress. Alethea is a novel proprietary technology composed of three key constituent compounds; the hitherto unexplored compound potassium dihydrojasmonate, an analogue of jasmonic acid; sodium benzoate, a carboxylic acid precursor to salicylic acid, and the α-amino acid L-arginine. Salinity treatment led to a maximal 47% reduction in net photosynthetic rate 8 d following NaCl treatment, yet in Alethea pre-treated seedlings, sensitivity to salinity stress was markedly reduced during the experimental period. Microarray analysis of leaf transcriptional responses showed that while salinity stress and Alethea individually impacted on largely non-overlapping, distinct groups of genes, Alethea pre-treatment substantially modified the response to salinity. Alethea affected the expression of genes related to biotic stress, ethylene signalling, cell wall synthesis, redox signalling and photosynthetic processes. Since Alethea had clear effects on photosynthesis/chloroplastic function at the physiological and molecular levels, we also investigated the ability of Alethea to protect various crop species against methyl viologen, a potent generator of oxidative stress in chloroplasts. Alethea pre-treatment produced dramatic reductions in visible foliar necrosis caused by methyl viologen compared with non-primed controls.
  
  
  CONCLUSIONS
  'Alethea' technology mediates positive recovery of abiotic stress-induced photosynthetic and foliar loss of performance, which is accompanied by altered transcriptional responses to stress.
authors.forename: Jason J
authors.initials: JJ
authors.lastname: Wargent
authors.forename: Douglas A
authors.initials: DA
authors.lastname: Pickup
authors.forename: Nigel D
authors.initials: ND
authors.lastname: Paul
authors.forename: Michael R
authors.initials: MR
authors.lastname: Roberts
mesh_terms: D018551:Solanum lycopersicum / Q000187:drug effects* / Q000235:genetics / Q000502:physiology*; D010788:Photosynthesis; D010937:Plant Growth Regulators / Q000494:pharmacology*; D018515:Plant Leaves / Q000187:drug effects / Q000502:physiology; D054712:Salinity; D013312:Stress, Physiological; D059467:Transcriptome
chemical_list: D010937:Plant Growth Regulators
references.citation: Plant Biol (Stuttg). 2008 Sep;10(5):563-72
references.pmid: 18761495
references.citation: J Sci Food Agric. 2010 Oct;90(13):2195-202
references.pmid: 20628998
references.citation: Nature. 2010 Apr 1;464(7289):788-91
references.pmid: 20360743
references.citation: Plant J. 2004 Mar;37(6):914-39
references.pmid: 14996223
references.citation: Plant Cell. 1996 Aug;8(8):1323-35
references.pmid: 8776899
references.citation: Nucleic Acids Res. 1989 Mar 25;17(6):2362
references.pmid: 2468132
references.citation: J Exp Bot. 2010;61(1):249-60
references.pmid: 19812243
references.citation: Proc Natl Acad Sci U S A. 2007 Dec 11;104(50):19703-8
references.pmid: 18077404
references.citation: Amino Acids. 2008 Jan;34(1):35-45
references.pmid: 17356805
references.citation: J Exp Bot. 2006;57(10):2391-400
references.pmid: 16766597
references.citation: FEBS Lett. 2004 Aug 27;573(1-3):83-92
references.pmid: 15327980
references.citation: J Biol Chem. 2004 Oct 29;279(44):45998-6007
references.pmid: 15322128
references.citation: Plant Cell. 2003 Jan;15(1):19-32
references.pmid: 12509519
references.citation: Plant J. 2005 Apr;42(2):201-17
references.pmid: 15807783
references.citation: Plant J. 2011 Feb;65(3):469-79
references.pmid: 21265899
references.citation: J Chem Ecol. 2005 Sep;31(9):1953-67
references.pmid: 16132206
references.citation: Plant J. 2005 Nov;44(4):653-68
references.pmid: 16262714
references.citation: J Exp Bot. 2007;58(3):507-20
references.pmid: 17210988
references.citation: J Plant Growth Regul. 1999 Oct;18(2):59-64
references.pmid: 10552133
references.citation: Plant Cell Environ. 2007 Apr;30(4):410-21
references.pmid: 17324228
references.citation: J Exp Bot. 2004 May;55(399):1115-23
references.pmid: 15047763
references.citation: Ann Bot. 2007 Jun;99(6):1161-73
references.pmid: 17428832
references.citation: Plant Physiol. 2005 Oct;139(2):822-35
references.pmid: 16183841
references.citation: J Exp Bot. 2011 Aug;62(12):4163-72
references.pmid: 21527627
references.citation: New Phytol. 2006;170(4):677-99
references.pmid: 16684231
references.citation: Plant Physiol. 2009 Dec;151(4):1902-17
references.pmid: 19812185
references.citation: Plant Cell Environ. 2011 Aug;34(8):1401-13
references.pmid: 21535014
references.citation: J Exp Bot. 2009;60(10):2827-38
references.pmid: 19380424
references.citation: Annu Rev Plant Biol. 2008;59:651-81
references.pmid: 18444910
references.citation: Trends Plant Sci. 2011 Jul;16(7):363-71
references.pmid: 21497543
references.citation: Plant Physiol. 2007 Feb;143(2):707-19
references.pmid: 17189334
references.citation: Plant Physiol. 2000 Aug;123(4):1337-50
references.pmid: 10938352
references.citation: Trends Plant Sci. 2011 May;16(5):249-57
references.pmid: 21216178
references.citation: Proc Natl Acad Sci U S A. 2011 Jul 26;108(30):12539-44
references.pmid: 21737749
references.citation: Funct Plant Biol. 2002 Jan;29(12):1393-1403
references.pmid: 32688739
references.citation: Plant Physiol. 2003 Aug;132(4):2098-107
references.pmid: 12913164
references.citation: Science. 2010 Feb 12;327(5967):812-8
references.pmid: 20110467
references.citation: Proc Natl Acad Sci U S A. 1998 Dec 8;95(25):14863-8
references.pmid: 9843981
references.citation: Plant Physiol. 2005 Sep;139(1):267-74
references.pmid: 16113213
references.citation: Mol Plant Microbe Interact. 2006 Oct;19(10):1062-71
references.pmid: 17022170
references.citation: Methods Enzymol. 2006;411:134-93
references.pmid: 16939790
references.citation: Plant Physiol. 2006 Jan;140(1):249-62
references.pmid: 16377744
references.citation: Plant Cell Environ. 2007 Sep;30(9):1041-51
references.pmid: 17661746
references.citation: Nucleic Acids Res. 2011 Jan;39(Database issue):D1156-63
references.pmid: 20965973
references.citation: Photosynth Res. 2004;82(1):17-33
references.pmid: 16228610