title: Preparation and characterization of DNA block copolymer assemblies loaded with nanoparticles.
abstract: We have recently developed a universal procedure to functionalize inorganic nanoparticles with a dense layer of DNA through the self-assembly of DNA block copolymers and nanoparticles. This functionalization strategy allows one to combine the useful physical properties of inorganic nanoparticle with the enhanced DNA binding properties that originate from the high surface DNA density. In particular, the hybrid nanostructures exhibit orders of magnitude higher binding constants than regular DNA strands. This chapter presents a detailed protocol for the preparation and characterization of DNA block copolymer assemblies loaded with nanoparticles.
authors.affiliation: Department of Chemistry, University of Pennsylvania, Philadelphia, PA, USA.
authors.forename: Xi-Jun
authors.initials: XJ
authors.lastname: Chen
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Hickey
authors.forename: So-Jung
authors.initials: SJ
authors.lastname: Park
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry; D004247:DNA / Q000737:chemistry*; D005290:Ferric Compounds / Q000737:chemistry; D008967:Molecular Biology / Q000379:methods*; D053758:Nanoparticles / Q000737:chemistry*; D049329:Nanostructures / Q000737:chemistry; D009841:Oligonucleotides / Q000737:chemistry; D011108:Polymers / Q000737:chemistry*; D011137:Polystyrenes / Q000737:chemistry; D013499:Surface Properties
chemical_list: D001672:Biocompatible Materials; D005290:Ferric Compounds; D009841:Oligonucleotides; D011108:Polymers; D011137:Polystyrenes; C000499:ferric oxide; D004247:DNA