title: [The analysis of hantavirus S gene in Apodemus agrarius in Changbai area].
abstract: To gain more insights into epidemiologic characteristics and genotype of hantavirus in Apodemus agrarius in Changbai Area. Complete hantavirus S segment sequences were amplified by RT-PCR and sequenced. The phylogenetic trees were constructed for analysis of genetic characters of hantavirus. A total of 58 Apodemus agrarius were trapped in the epidemic areas, and complete hantavirus S segment sequences were obtained from 4 lung samples of these rodents (6. 90%0). Phylogenetic analysis of the four S segment sequences indicated that all viruses isolated from Apodemu sagrarius were closely related to genotype 6 of Hantaan virus (95. 8%-96. 3%, nucleotide identity; 98. 6%-99. 5%, amino acid identity), all of them had a specific S387 different from other genotypes of Hantaan virus.
authors.affiliation: Chinese Academy of Inspection and Quarantine, Beijing 100123,China. yql0610@163.com
authors.forename: Qing-Li
authors.initials: QL
authors.lastname: Yan
authors.forename: Peng-Feil
authors.initials: PF
authors.lastname: Yang
authors.forename: Li-Jun
authors.initials: LJ
authors.lastname: Shao
authors.forename: Yong-Xian
authors.initials: YX
authors.lastname: Liu
authors.forename: Yun
authors.initials: Y
authors.lastname: Pu
authors.forename: Xiao-Long
authors.initials: XL
authors.lastname: Zhang
authors.forename: Xiao-Mei
authors.initials: XM
authors.lastname: Cao
authors.forename: Tian-Yu
authors.initials: TY
authors.lastname: Guo
authors.forename: Li-Si
authors.initials: LS
authors.lastname: Yao
mesh_terms: D000818:Animals; D001483:Base Sequence; D002681:China / Q000453:epidemiology; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D004197:Disease Reservoirs / Q000821:virology*; D005838:Genotype; D006476:Orthohantavirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D018778:Hantavirus Infections / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D008168:Lung / Q000821:virology; D051189:Murinae / Q000821:virology*; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012376:Rodent Diseases / Q000821:virology*; D017422:Sequence Analysis, DNA; D014764:Viral Proteins / Q000235:genetics*
chemical_list: D018076:DNA, Complementary; D012367:RNA, Viral; D014764:Viral Proteins