title: Increase of circulating CD11b(+)Gr1(+) cells and recruitment into the synovium in osteoarthritic mice with hyperlipidemia.
abstract: Although recent studies suggest that hyperlipidemia is a risk factor for osteoarthritis (OA), the link between OA and hyperlipidemia is not fully understood. As the number of activated, circulating myeloid cells is increased during hyperlipidemia, we speculate that myeloid cells contribute to the pathology of OA. Here, we characterized myeloid cells in STR/Ort mice, a murine osteoarthritis model, under hyperlipidemic conditions. Ratios of myeloid cells in bone marrow, the spleen, and peripheral blood were determined by flow cytometry. To examine the influence of the hematopoietic environment, including abnormal stem cells, on the hematopoietic profile of STR/Ort mice, bone marrow transplantations were performed. The relationship between hyperlipidemia and abnormal hematopoiesis was examined by evaluating biochemical parameters and spleen weight of F2 animals (STR/Ort x C57BL/6J). In STR/Ort mice, the ratio of CD11b(+)Gr1(+) cells in spleens and peripheral blood was increased, and CD11b(+)Gr1(+) cells were also present in synovial tissue. Splenomegaly was observed and correlated with the ratio of CD11b(+)Gr1(+) cells. When bone marrow from GFP-expressing mice was transplanted into STR/Ort mice, no difference in the percentage of CD11b(+)Gr1(+) cells was observed between transplanted and age-matched STR/Ort mice. Analysis of biochemical parameters in F2 mice showed that spleen weight correlated with serum total cholesterol. These results suggest that the increase in circulating and splenic CD11b(+)Gr1(+) cells in STR/Ort mice originates from hypercholesterolemia. Further investigation of the function of CD11b(+)Gr1(+) cells in synovial tissue may reveal the pathology of OA in STR/Ort mice.
authors.affiliation: Department of Orthopedic Surgery, Kitasato University School of Medicine, 1-15-1 Minami-ku Kitasato, Sagamihara City, Kanagawa 252-0374, Japan.
authors.forename: Kentaro
authors.initials: K
authors.lastname: Uchida
authors.forename: Kouji
authors.initials: K
authors.lastname: Naruse
authors.forename: Masashi
authors.initials: M
authors.lastname: Satoh
authors.forename: Kenji
authors.initials: K
authors.lastname: Onuma
authors.forename: Masaki
authors.initials: M
authors.lastname: Ueno
authors.forename: Shotaro
authors.initials: S
authors.lastname: Takano
authors.forename: Ken
authors.initials: K
authors.lastname: Urabe
authors.forename: Masashi
authors.initials: M
authors.lastname: Takaso
mesh_terms: D000818:Animals; D000950:Antigens, Ly; D039481:CD11b Antigen; D002465:Cell Movement; D006949:Hyperlipidemias / Q000150:complications / Q000276:immunology / Q000473:pathology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008815:Mice, Inbred Strains; D022423:Myeloid Cells / Q000276:immunology* / Q000473:pathology*; D010003:Osteoarthritis / Q000150:complications / Q000276:immunology / Q000473:pathology*; D013583:Synovial Membrane / Q000166:cytology* / Q000473:pathology*
chemical_list: D000950:Antigens, Ly; D039481:CD11b Antigen; C480309:Ly6G antigen, mouse
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