title: The influence of amylose-LPC complex formation on the susceptibility of wheat starch to amylase.
abstract: This study was aimed to assess the role of lysophosphatidylcholine (LPC) in the development of slowly digestible starch (SDS). The influence of LPC, on the enzymatic degradation of diluted 9% wheat starch suspensions (w/w) was investigated, using an in vitro digestion method. Wheat starch suspensions containing 0.5-5% LPC (based on starch) were heated in a Rapid Visco Analyser (RVA) till 95 °C and subjected to enzyme hydrolysis by porcine pancreatic α-amylase at 37 °C for several digestion periods. In vitro digestion measurements demonstrated that complexing starch with 5% LPC leads to a 22% decrease in rate of reducing sugar compared to the reference while the samples containing 0.5% LPC showed an equal digestibility comparable to the control. A clear decrease in the formation of reducing sugars was observed in presence of 2-5% LPC, since the results after 15 min digestion imply the formation of SDS due to the formation of amylose-LPC inclusion complexes. The DSC measurements proved the presence of amylose-LPC inclusion complexes even after 240 min digestion demonstrating the low susceptibility of amylose-V complexes to amylase.
authors.affiliation: Department of Polymer Chemistry, Zernike Institute for Advanced Materials, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands.
authors.forename: S
authors.initials: S
authors.lastname: Ahmadi-Abhari
authors.forename: A J J
authors.initials: AJ
authors.lastname: Woortman
authors.forename: A A C M
authors.initials: AA
authors.lastname: Oudhuis
authors.forename: R J
authors.initials: RJ
authors.lastname: Hamer
authors.forename: K
authors.initials: K
authors.lastname: Loos
mesh_terms: D000688:Amylose / Q000378:metabolism*; D000818:Animals; D008244:Lysophosphatidylcholines / Q000378:metabolism*; D013213:Starch / Q000378:metabolism*; D034421:Sus scrofa; D013696:Temperature; D013997:Time Factors; D014908:Triticum / Q000378:metabolism*; D014783:Viscosity; D000516:alpha-Amylases / Q000378:metabolism*
chemical_list: D008244:Lysophosphatidylcholines; D013213:Starch; D000688:Amylose; D000516:alpha-Amylases
keywords: Amylose inclusion complexation; Digestibility; Enzymatic hydrolysis; Lysophosphatidylcholine; Wheat starch