title: Comparison between conidia and blastospores of Esteya vermicola, an endoparasitic fungus of the pinewood nematode, Bursaphelenchus xylophilus.
abstract: Esteya vermicola, an endoparasitic fungus of Bursaphelenchus xylophilus, the pinewood nematode (PWN), exhibits great potential as a biological control agent against this nematode. E. vermicola produces blastospores in liquid media and aerial conidia on solid media. The agent was mass-produced using two kinds of culture media: S (50 % wheat bran and 50 % pine wood powder), L (0.5 g wheat bran and 0.5 g pinewood powder in 200 ml of potato dextrose broth), and two controls: SC (potato dextrose agar), LC (potato dextrose broth). Yields, multiple stress tolerance, storage life, new generation conidial number, and PWN mortality rates of the spores were measured in each of these four media and compared. The spore yields, new generation conidial number, and nematode mortality rates of blastospores were higher than those of conidia. Nevertheless, the conidia had a higher germination rate than the blastospores during the storage process and multiple stress treatments. Considering the number of spores surviving from the process of the storage and multiple stress treatments per unit of mass media, the blastospores from L survived most. Comprehensive analysis indicates that the L culture medium is the most optimal medium for mass production relatively.
authors.affiliation: Department of Food Science and Technology, College of Agriculture and Biotechnology, Chungnam National University, Daejeon, South Korea.
authors.forename: Yun-Bo
authors.initials: YB
authors.lastname: Wang
authors.forename: Zhi-Hong
authors.initials: ZH
authors.lastname: Yang
authors.forename: Jiao-Jiao
authors.initials: JJ
authors.lastname: Yu
authors.forename: Yong-An
authors.initials: YA
authors.lastname: Zhang
authors.forename: Jian-Jie
authors.initials: JJ
authors.lastname: Xue
authors.forename: Zheng
authors.initials: Z
authors.lastname: Li
authors.forename: Jing-Jie
authors.initials: JJ
authors.lastname: Li
authors.forename: Chun-Yan
authors.initials: CY
authors.lastname: Wang
authors.forename: Zhen
authors.initials: Z
authors.lastname: Wang
authors.forename: Jin-Gang
authors.initials: JG
authors.lastname: Hou
authors.forename: Shahraz
authors.initials: S
authors.lastname: Begum
authors.forename: Li-Juan
authors.initials: LJ
authors.lastname: Gu
authors.forename: Mi-Ra
authors.initials: MR
authors.lastname: Lee
authors.forename: Chang-Keun
authors.initials: CK
authors.lastname: Sung
mesh_terms: D000818:Animals; D001203:Ascomycota / Q000502:physiology*; D003470:Culture Media; D005285:Fermentation; D028223:Pinus / Q000469:parasitology; D010935:Plant Diseases / Q000469:parasitology; D013172:Spores, Fungal / Q000502:physiology*; D013312:Stress, Physiological; D017187:Tylenchida / Q000254:growth & development / Q000382:microbiology*; D014934:Wood / Q000469:parasitology
chemical_list: D003470:Culture Media
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