title: Canonical Wnts, specifically Wnt-10b, show ability to maintain dermal papilla cells.
abstract: Although Wnts are expressed in hair follicles (HFs) and considered to be crucial for maintaining dermal papilla (DP) cells, the functional differences among them remain largely unknown. In the present study, we investigated the effects of Wnts (Wnt-3a, 5a, 10b, 11) on the proliferation of mouse-derived primary DP cells in vitro as well as their trichogenesis-promoting ability using an in vivo skin reconstitution protocol. Wnt-10b promoted cell proliferation and trichogenesis, while Wnt-3a showed those abilities to a limited extent, and Wnt-5a and 11 had no effects. Furthermore, we investigated the effects of these Wnts on cultured DP cells obtained from versican-GFP transgenic mice and found that Wnt-10b had a potent ability to sustain their GFP-positivity. These results suggest that canonical Wnts, specifically Wnt-10b, play important roles in the maintenance of DP cells and trichogenesis.
authors.affiliation: Department of Pathogen, Infection and Immunity, Nara Medical University, Kashihara, Nara, Japan. oujix@naramed-u.ac.jp
authors.forename: Yukiteru
authors.initials: Y
authors.lastname: Ouji
authors.forename: Fukumi
authors.initials: F
authors.lastname: Nakamura-Uchiyama
authors.forename: Masahide
authors.initials: M
authors.lastname: Yoshikawa
mesh_terms: D000818:Animals; D019556:COS Cells; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D020405:Dermis / Q000166:cytology*; D018859:Hair Follicle / Q000166:cytology* / Q000378:metabolism; D051379:Mice; D008822:Mice, Transgenic; D051153:Wnt Proteins / Q000502:physiology*; D060509:Wnt3A Protein / Q000502:physiology
chemical_list: D051153:Wnt Proteins; C102068:Wnt10b protein, mouse; D060509:Wnt3A Protein; C555511:Wnt3a protein, mouse
keywords: Canonical pathway; Dermal papilla; Wnt signaling; Wnt-10b