title: [Autophagy promoted by Pakinson's disease related protein Pink1].
abstract: OBJECTIVE
  To investigate the function of Parkinson's disease (PD)-related protein Pink1 in autophagy.
  
  
  METHODS
  Pink1, autophagy inhibitor Bcl-XL and autophagy induced-factor Beclin1 were amplified with RT-PCR and contructed into pcDNA3. 1 (+) vector. The stable HEK293 cell line of Pink1 expression was established through the lentivirus system. Pink1, Bcl-XL and Beclin1 were tansfected into the HEK293 Cell Line. Co-Immunoprecipitation and Western blot were performed to determine the interaction between Pink1 and Bcl-XL, the effect of Pink1 on the interaction between Bcl-XL and Beclin1, and the function of Pink1 in autophagy.
  
  
  RESULTS
  There was a new interaction between Pink1 and antophagy inhibitor Bcl-XL. Overexpressed Pink1 promoted the dissociation of autophagy induced-factor Beclin1 from Bcl-XL. Overexpressed Pink1 increased the autophagic protein LC3 II/LC3 I.
  
  
  CONCLUSIONS
  Pakinson's disease related protein Pink1 promotes the dissociation of autophagy induced-factor Beclin1 from antophagy inhibitor Bcl-XL and promotes autophagy.
authors.affiliation: Development Stem Cell Institute, West China Second Hospital, Sichuan University, Chengdu 610041, China.
authors.forename: Ming-Ming
authors.initials: MM
authors.lastname: Tong
authors.forename: Chang-An
authors.initials: CA
authors.lastname: Jiang
mesh_terms: D051017:Apoptosis Regulatory Proteins / Q000378:metabolism; D001343:Autophagy / Q000502:physiology*; D000071186:Beclin-1; D006801:Humans; D008565:Membrane Proteins / Q000378:metabolism; D010300:Parkinson Disease / Q000378:metabolism / Q000503:physiopathology*; D011494:Protein Kinases / Q000378:metabolism*; D051020:bcl-X Protein / Q000378:metabolism; D000099202:PTEN-Induced Putative Kinase
chemical_list: D051017:Apoptosis Regulatory Proteins; D000071186:Beclin-1; D008565:Membrane Proteins; D011494:Protein Kinases; D051020:bcl-X Protein; D000099202:PTEN-Induced Putative Kinase; C494811:BCL2L1 protein, human; C491997:BECN1 protein, human