title: Bmp4 expressed in preadipocytes is required for the onset of adipocyte differentiation.
abstract: We previously revealed that endogenous bone morphogenetic protein (Bmp) activity is required for lipid accumulation in 3T3-L1 adipocytes. The present study characterized the role of endogenous Bmp activity in preadipocytes. Endogenous Bmp activity was monitored by analyzing the level of phosphorylation of Smad1/5/8, downstream molecules in the Bmp pathway. Higher levels of phosphorylated Smad1/5/8 were detected in adipogenic cells but not in non-adipogenic cells prior to differentiation induction. The inhibition of the Bmp pathway during this period decreased the expression of Pparγ2 and C/ebpα, which are transcription factors responsible for adipocyte differentiation. The expression of these transcription factors were also down-regulated by Bmp4 knockdown. In addition, endogenous Bmp4 was required for the repression of Intrleukin-11 expression. Endogenous Bmp4 in preadipocytes is indispensable for the onset of the adipogenic program, and may help to maintain the preadipocytic state during adipocyte differentiation.
authors.affiliation: Division of Applied Biosciences, Kyoto University Graduate School of Agriculture, Kyoto 606-8502, Japan.
authors.forename: Masashi
authors.initials: M
authors.lastname: Suenaga
authors.forename: Norio
authors.initials: N
authors.lastname: Kurosawa
authors.forename: Hiroki
authors.initials: H
authors.lastname: Asano
authors.forename: Yohei
authors.initials: Y
authors.lastname: Kanamori
authors.forename: Takenao
authors.initials: T
authors.lastname: Umemoto
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Yoshida
authors.forename: Masaru
authors.initials: M
authors.lastname: Murakami
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Kawachi
authors.forename: Tohru
authors.initials: T
authors.lastname: Matsui
authors.forename: Masayuki
authors.initials: M
authors.lastname: Funaba
mesh_terms: D016475:3T3 Cells; D017667:Adipocytes / Q000166:cytology* / Q000378:metabolism; D050156:Adipogenesis; D000818:Animals; D055396:Bone Morphogenetic Protein 2 / Q000037:antagonists & inhibitors / Q000378:metabolism; D055415:Bone Morphogenetic Protein 4 / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D022762:CCAAT-Enhancer-Binding Proteins / Q000096:biosynthesis / Q000378:metabolism; D002417:Cattle; D002454:Cell Differentiation; D002460:Cell Line; D019070:Cell Lineage; D017370:Interleukin-11 / Q000096:biosynthesis; D051379:Mice; D047495:PPAR gamma / Q000096:biosynthesis / Q000378:metabolism; D010766:Phosphorylation / Q000187:drug effects; D011720:Pyrazoles / Q000494:pharmacology; D011743:Pyrimidines / Q000494:pharmacology; D051898:Smad1 Protein / Q000378:metabolism; D051902:Smad5 Protein / Q000378:metabolism; D051904:Smad8 Protein / Q000378:metabolism; D015533:Transcriptional Activation / Q000187:drug effects
chemical_list: C525719:Bmp2 protein, mouse; C525810:Bmp4 protein, mouse; D055396:Bone Morphogenetic Protein 2; D055415:Bone Morphogenetic Protein 4; D022762:CCAAT-Enhancer-Binding Proteins; C530718:CEBPA protein, mouse; D017370:Interleukin-11; C554430:LDN 193189; D047495:PPAR gamma; D011720:Pyrazoles; D011743:Pyrimidines; D051898:Smad1 Protein; C497401:Smad1 protein, mouse; D051902:Smad5 Protein; C497798:Smad5 protein, mouse; D051904:Smad8 Protein; C491621:Smad9 protein, mouse; C516138:dorsomorphin
keywords: Adipocyte differentiation; Bmp4; Interleukin-11; Preadipocytes