title: Basic DNA electrophoresis in molecular cloning: a comprehensive guide for beginners.
abstract: Presented here is a complete molecular cloning protocol consisting of a number of separate but interconnected methods such as preparation of E. coli competent cells; in vitro DNA digestion and ligation; PCR; DNA agarose gel electrophoresis and gel extraction; and screening transformants by colony PCR, analytical restriction digests, and sequencing. The method is described in a lot of details so that it can be easily followed by those with very little relevant knowledge and skills. It also contains many tips that even experienced researchers may find useful.
authors.affiliation: Institute of Cell Biology, University of Edinburgh, Edinburgh, UK.
authors.forename: Svetlana
authors.initials: S
authors.lastname: Makovets
mesh_terms: D003001:Cloning, Molecular / Q000379:methods*; D004247:DNA / Q000737:chemistry / Q000302:isolation & purification*; D004586:Electrophoresis / Q000379:methods*; D004926:Escherichia coli / Q000235:genetics; D017422:Sequence Analysis, DNA / Q000379:methods; D014169:Transformation, Bacterial / Q000235:genetics
chemical_list: D004247:DNA