title: Flow-enhanced electrochemical immunosensors on centrifugal microfluidic platforms.
abstract: We present a novel fully integrated centrifugal microfluidic device with features for target antigen capture from biological samples, via a bead-based enzyme-linked immune-sorbent assay, and flow-enhanced electrochemical detection. The limit of detection (LOD) of our device for the C-reactive protein (CRP) was determined to be 4.9 pg mL(-1), a 17-fold improvement over quantification by optical density. The complete sample-to-answer protocol of our device is fully automated and takes less than 20 min. Overall, the presented microfluidic disc adds to the comparatively small number of fully integrated microfluidic-based platforms that utilize electrochemical detection and exemplifies how electrochemical detection can be enhanced by flow to successfully detect very low levels of biomarkers (e.g. pg mL(-1)).
authors.affiliation: Department of Biomedical Engineering, Ulsan National Institute of Science and Technology (UNIST), Ulsan, South Korea.
authors.forename: Tae-Hyeong
authors.initials: TH
authors.lastname: Kim
authors.forename: Kameel
authors.initials: K
authors.lastname: Abi-Samra
authors.forename: Vijaya
authors.initials: V
authors.lastname: Sunkara
authors.forename: Dong-Kyu
authors.initials: DK
authors.lastname: Park
authors.forename: Mary
authors.initials: M
authors.lastname: Amasia
authors.forename: Nahui
authors.initials: N
authors.lastname: Kim
authors.forename: Jintae
authors.initials: J
authors.lastname: Kim
authors.forename: Hanshin
authors.initials: H
authors.lastname: Kim
authors.forename: Marc
authors.initials: M
authors.lastname: Madou
authors.forename: Yoon-Kyoung
authors.initials: YK
authors.lastname: Cho
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology; D001331:Automation; D015415:Biomarkers / Q000032:analysis; D015374:Biosensing Techniques; D002097:C-Reactive Protein / Q000032:analysis / Q000276:immunology; D002498:Centrifugation; D055664:Electrochemical Techniques; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D051379:Mice; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods
chemical_list: D000906:Antibodies; D015415:Biomarkers; D002097:C-Reactive Protein