title: Detecting intracellular cysteine redox states by in-cell NMR spectroscopy.
abstract: An in-cell perspective: Nowadays, in-cell NMR spectroscopy has proven to be a thrilling alternative for the investigation of biomacromolecules under physiological conditions at atomic resolution. A recent example demonstrating significant progress in in-cell NMR was published by the groups of Banci and Aricescu that investigated the post-translational maturation of human superoxide dismutase 1 (SOD1) in living human cells.
authors.affiliation: Institute for Organic Chemistry and Chemical Biology, Center of Biomolecular Magnetic Resonance, Goethe University Frankfurt, Max-von-Laue-Strasse 7, 60438 Frankfurt am Main (Germany).
authors.forename: Robert
authors.initials: R
authors.lastname: Silvers
authors.forename: Harald
authors.initials: H
authors.lastname: Schwalbe
mesh_terms: D003545:Cysteine / Q000737:chemistry* / Q000378:metabolism; D004926:Escherichia coli / Q000737:chemistry / Q000378:metabolism; D057809:HEK293 Cells; D006801:Humans; D018832:Molecular Chaperones / Q000235:genetics; D019906:Nuclear Magnetic Resonance, Biomolecular; D010084:Oxidation-Reduction; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics; D013482:Superoxide Dismutase / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D000072105:Superoxide Dismutase-1
chemical_list: C114678:CCS protein, human; D018832:Molecular Chaperones; D011994:Recombinant Proteins; C000606290:SOD1 protein, human; D013482:Superoxide Dismutase; D000072105:Superoxide Dismutase-1; D003545:Cysteine
keywords: NMR; eukaryotic proteins; in-cell NMR spectroscopy; protein maturation