title: Sysmex UF-1000i performance for screening yeasts in urine.
abstract: We tested the capacity of the Sysmex UF-1000i system to detect yeasts in urine by screening a total of 22 132 urine samples received for culture in our microbiology laboratory during 1 year. We also analyzed different dilutions of previously filtered urine inoculated with a strain of Candida albicans. With clinical samples, a single cut-off point of 50 yeast-like cells (YLCs)/μL detected candiduria ≥10 000 colony forming units (CFU)/mL and >100 000 CFU/mL with a sensitivity of 87.3%/95.4%, a specificity of 97%, a negative predictive value of 95.9%, and a positive predictive value of 9.3%/5.7%. With the simulated samples, a linear relationship was observed between the dilution factor and the number of cells detected by UF-1000i. This instrument appears to be able to reliably rule out candiduria of a magnitude of at least 10 000 CFU/mL and facilitate urine sample screening, thereby providing fast results. The Sysmex UF1000i system can be adapted for candiduria screening by the use of an appropriate YLCs/μL cut-off point that takes account of the prevalence of candiduria in the population.
authors.affiliation: Microbiology Area, University Hospital Virgen de las Nieves, Granada; Faculty of Medicine, University of Granada, Granada.
authors.forename: José
authors.initials: J
authors.lastname: Gutiérrez-Fernández
authors.forename: Cristina
authors.initials: C
authors.lastname: Riazzo
authors.forename: Sara
authors.initials: S
authors.lastname: Sanbonmatsu
authors.forename: Juan
authors.initials: J
authors.lastname: de Dios Luna
authors.forename: Antonio
authors.initials: A
authors.lastname: Sorlózano
authors.forename: Consuelo
authors.initials: C
authors.lastname: Miranda
authors.forename: José María
authors.initials: JM
authors.lastname: Navarro
mesh_terms: D000328:Adult; D000368:Aged; D057205:Automation, Laboratory; D002177:Candidiasis / Q000175:diagnosis / Q000382:microbiology; D015169:Colony Count, Microbial / Q000379:methods / Q000706:statistics & numerical data; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D009172:Mycology / Q000379:methods* / Q000706:statistics & numerical data; D009181:Mycoses / Q000175:diagnosis / Q000382:microbiology; D011247:Pregnancy; D014552:Urinary Tract Infections / Q000175:diagnosis* / Q000382:microbiology*; D014556:Urine / Q000382:microbiology*; D015003:Yeasts / Q000302:isolation & purification*
keywords: Candida albicans; Urine; automated system; screening; yeast