title: Comparison of inhibitory activities and mechanisms of five mulberry plant bioactive components against α-glucosidase.
abstract: The α-glucosidase inhibitory effects of five bioactive components, namely 1-deoxynojirimycin, cyanidin-3-glucoside, cyanidin-3-rutinoside, resveratrol and oxyresveratrol contained in mulberry (Morus, Moraceae) plants have been compared. Spectroscopy methods were employed to compare their α-glucosidase inhibitory mechanisms. The results revealed that 1-deoxynojirimycin (competitive), resveratrol and oxyresveratrol (noncompetitive) were stronger inhibitors than acarbose, while cyanidin-3-glucoside and cyanidin-3-rutinoside (mix competitive and noncompetitive) showed modest activities. 1-Deoxynojirimycin, resveratrol and oxyresveratrol could quench the fluorescence spectra statically by forming stable complexes, while the quenching of cyanidin-3-rutinoside and cyanidin-3-glucoside belonged to dynamic quenching by the collision of molecules. The interactions between ligands and α-glucosidase were mainly driven by hydrophobic force, or hydrogen bonding consequently induced conformational changes and reduced surface hydrophobicity. Docking results suggested that they could bind to α-glucosidase at different sites. This work provides useful information for the understanding of the ligands-α-glucosidase interactions and identifies oxyresveratrol as a potent α-glucosidase inhibitor.
authors.affiliation: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, PR China.
authors.forename: Hao
authors.initials: H
authors.lastname: He
authors.forename: Yan-Hua
authors.initials: YH
authors.lastname: Lu
mesh_terms: D004791:Enzyme Inhibitors / Q000737:chemistry*; D065089:Glycoside Hydrolase Inhibitors; D007700:Kinetics; D031625:Morus / Q000737:chemistry* / Q000145:classification; D010936:Plant Extracts / Q000737:chemistry*; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D029701:Saccharomyces cerevisiae Proteins / Q000037:antagonists & inhibitors* / Q000737:chemistry; D000520:alpha-Glucosidases / Q000737:chemistry
chemical_list: D004791:Enzyme Inhibitors; D065089:Glycoside Hydrolase Inhibitors; D010936:Plant Extracts; D029701:Saccharomyces cerevisiae Proteins; D000520:alpha-Glucosidases