title: Protein preparation, crystallization and preliminary X-ray analysis of Polygonum cuspidatum bifunctional chalcone synthase/benzalacetone synthase.
abstract: The chalcone synthase (CHS) superfamily of type III polyketide synthases (PKSs) generate the backbones of a variety of plant secondary metabolites. An active bifunctional chalcone synthase/benzalacetone synthase (CHS/BAS) from Polygonum cuspidatum was overexpressed in Escherichia coli as a C-terminally polyhistidine-tagged fusion protein, purified to homogeneity and crystallized using polyethylene glycol 4000 as a precipitant. The production of well shaped crystals of the complex between PcPKS1 and benzalacetone was dependent on the presence of sorbitol and barium chloride as additives. The crystals belonged to the orthorhombic space group P2₁2₁2₁, with unit-cell parameters a = 80.23, b = 81.01, c = 122.89 Å, and diffracted X-rays to at least 2.0 Å resolution.
authors.affiliation: Key Laboratory of Urban Agriculture (North) of Chinese Ministry of Agriculture, Beijing University of Agriculture, 7 Beinong Road, Beijing 102206, People's Republic of China.
authors.forename: Heshu
authors.initials: H
authors.lastname: Lu
authors.forename: Mingfeng
authors.initials: M
authors.lastname: Yang
authors.forename: Chunmei
authors.initials: C
authors.lastname: Liu
authors.forename: Ping
authors.initials: P
authors.lastname: Lu
authors.forename: Huaixing
authors.initials: H
authors.lastname: Cang
authors.forename: Lanqing
authors.initials: L
authors.lastname: Ma
mesh_terms: D000217:Acyltransferases / Q000737:chemistry* / Q000235:genetics; D000595:Amino Acid Sequence; D002074:Butanones / Q000737:chemistry*; D003460:Crystallization; D045468:Fallopia japonica / Q000201:enzymology* / Q000235:genetics; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000737:chemistry* / Q000235:genetics; D014961:X-Ray Diffraction
chemical_list: D002074:Butanones; D010940:Plant Proteins; C025383:benzylideneacetone; D000217:Acyltransferases; C020141:flavanone synthetase
keywords: Polygonum cuspidatum; chalcone synthase/benzalacetone synthase
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