title: Influences of magnetized hydroxyapatite on the growth behaviors of osteoblasts and the mechanism from molecular dynamics simulation.
abstract: To investigate the influence of magnetized hydroxyapatite on the growth and differentiation of osteoblasts, hydroxyapatite (HA) and magnetized hydroxyapatite (mHA) were synthesized and characterized. The cell viability, differentiation, and morphologies of osteoblasts were investigated in vitro, respectively. The results showed that compared to HA, cells cultured with mHA had better cell viability, and both HA and mHA were beneficial to the early differentiation of osteoblasts. Furthermore, the interaction mechanism between mHA and osteoblasts was elucidated using a molecular dynamics simulation. The simulation results indicated that when cultured with osteoblasts, HA adsorbed bovine serum protein onto its surface from the medium immediately, which was beneficial to the adhesion and proliferation of osteoblasts. The main driving force for the adsorption of bovine serum was the electronic properties of HA crystal faces. The (211) crystal face of HA had the highest electron density among its all crystal faces, thus mainly contributing to the protein adsorption of HA. Nevertheless, the (211) crystal face of mHA still had a relatively higher electron density than that of HA, thus possessing better protein adsorption than that of HA, and in turn promoting the biological functions of osteoblasts.
authors.affiliation: Key Laboratory of Biorheological Science and Technology, Ministry of Education College of Bioengineering, Chongqing University, Chongqing 400044, China.
authors.forename: Weihu
authors.initials: W
authors.lastname: Yang
authors.forename: Xingfeng
authors.initials: X
authors.lastname: Xi
authors.forename: Jiajia
authors.initials: J
authors.lastname: Fang
authors.forename: Peng
authors.initials: P
authors.lastname: Liu
authors.forename: Kaiyong
authors.initials: K
authors.lastname: Cai
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D000831:Animals, Newborn; D002417:Cattle; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D048429:Cell Size / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003460:Crystallization; D003599:Cytoskeleton / Q000187:drug effects / Q000378:metabolism; D017886:Durapatite / Q000494:pharmacology*; D060328:Magnetic Phenomena; D056004:Molecular Dynamics Simulation; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects / Q000201:enzymology; D051381:Rats; D012710:Serum Albumin, Bovine / Q000378:metabolism; D017550:Spectroscopy, Fourier Transform Infrared; D055672:Static Electricity; D014961:X-Ray Diffraction
chemical_list: D012710:Serum Albumin, Bovine; D017886:Durapatite; D000469:Alkaline Phosphatase
keywords: Biointerface; Cytocompatibility; Magnetized hydroxyapatite; Molecular dynamics simulation; Osteoblasts