title: Germinated brown rice extract inhibits adipogenesis through the down-regulation of adipogenic genes in 3T3-L1 adipocytes.
abstract: The aim of this study was to examine the anti-adipogenic effect of germinated brown rice methanol extract (GBR) in 3T3-L1 adipocytes. The GBR inhibited adipocyte differentiation was measured by Oil Red O staining and glycerol-3-phosphate dehydrogenase (GPDH) activity in a dose-dependent manner without initiating any cytotoxicity. The mRNA levels of adipogenic transcription factors such as CCAAT/enhancer binding protein (C/EBPα), proliferator-activated receptorγ (PPARγ), and sterol regulatory element-binding protein-1c (SREBP-1c), and adipogenic genes, such as fatty acid synthase (FAS), adipocyte fatty acid-binding protein (aP2), and lipoprotein lipase (LPL), were significantly down-regulated by treatment with GBR when compared to that of untreated control cells. Moreover, tumor necrosis factor-α (TNF-α) and interlukin-6 (IL-6) mRNA expressions were attenuated by GBR in mature adipocytes. These data suggest that GBR exhibits an anti-adipogenic effect through the suppression of adipogenesis in 3T3-L1 adipocytes.
authors.affiliation: Department of Medical Nutrition, Kyung Hee University, Yongin, Korea.
authors.forename: Jin-Nyoung
authors.initials: JN
authors.lastname: Ho
authors.forename: Mi-Eun
authors.initials: ME
authors.lastname: Son
authors.forename: Won-Chul
authors.initials: WC
authors.lastname: Lim
authors.forename: Seung-Taik
authors.initials: ST
authors.lastname: Lim
authors.forename: Hong-Yon
authors.initials: HY
authors.lastname: Cho
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D050156:Adipogenesis / Q000187:drug effects / Q000235:genetics*; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D015536:Down-Regulation; D064429:Fatty Acid Synthases / Q000235:genetics; D050556:Fatty Acid-Binding Proteins / Q000235:genetics; D018525:Germination; D005993:Glycerolphosphate Dehydrogenase / Q000378:metabolism; D008071:Lipoprotein Lipase / Q000235:genetics; D051379:Mice; D012275:Oryza / Q000737:chemistry*; D010936:Plant Extracts / Q000494:pharmacology*; D012333:RNA, Messenger / Q000032:analysis; D012639:Seeds / Q000737:chemistry* / Q000254:growth & development; D014157:Transcription Factors / Q000235:genetics
chemical_list: C493411:Fabp4 protein, mouse; D050556:Fatty Acid-Binding Proteins; D010936:Plant Extracts; D012333:RNA, Messenger; D014157:Transcription Factors; D005993:Glycerolphosphate Dehydrogenase; D064429:Fatty Acid Synthases; D008071:Lipoprotein Lipase
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