title: Optimum extraction of polysaccharides from Opuntia dillenii and evaluation of its antioxidant activities.
abstract: Response surface methodology (RSM) was applied to optimize the extraction of crude polysaccharides from Opuntia dillenii (Ker-Gaw) Haw. A three-level, four-variable Box-Behnken design was employed to obtain the best possible combination of extraction temperature (80-90 °C), extraction time (50-70 min), number of extraction cycle (1-3 times), and ratio of water to raw material (8:1-12:1, v/w) for maximum yield of crude polysaccharide. Besides, the antioxidant capacity of crude polysaccharide was evaluated by DPPH assay. The results showed that optimized extraction conditions were extraction temperature 85 °C, extraction time 63.7 min, extraction 2 times and ratio of water to raw material 11.14:1. Under these conditions, the experimental yield was 27.36 ± 0.21%, which is well in close agreement with the value (27.44%) predicted by RSM model. Pharmacological test showed that O. dillenii crude polysaccharides had a good antioxidant activity.
authors.affiliation: College of Urban Construction and Environmental Engineering, Chongqing University, Chongqing 400030, PR China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Yang
authors.forename: Huaguo
authors.initials: H
authors.lastname: Chen
authors.forename: Xin
authors.initials: X
authors.lastname: Zhou
authors.forename: Junzeng
authors.initials: J
authors.lastname: Zhang
mesh_terms: D000704:Analysis of Variance; D000975:Antioxidants / Q000494:pharmacology*; D029747:Opuntia / Q000737:chemistry*; D011134:Polysaccharides / Q000302:isolation & purification* / Q000494:pharmacology*; D015203:Reproducibility of Results; D012997:Solvents; D013696:Temperature; D013997:Time Factors; D014867:Water
chemical_list: D000975:Antioxidants; D011134:Polysaccharides; D012997:Solvents; D014867:Water
keywords: Antioxidant activity; Extraction; Opuntia dillenii; Polysaccharides; Response surface methodology