title: The comet assay in marine animals.
abstract: Comet assay is a quick and versatile technique for assessing DNA damage in individual cells. It allows the detection of DNA single- and double-strand breaks, as well as the presence of alkali-labile sites and cross-links. Here we describe the protocols for the single-cell gel electrophoresis (Comet) assay in its alkaline (pH > 13), mild alkaline (pH = 12.1), and neutral (pH = 8) versions, when applied in marine animals.
authors.affiliation: Department of Clinical and Experimental Medicine, University of Pisa, Pisa, Italy.
authors.forename: Giada
authors.initials: G
authors.lastname: Frenzilli
authors.forename: Brett P
authors.initials: BP
authors.lastname: Lyons
mesh_terms: D000818:Animals; D059001:Aquatic Organisms / Q000235:genetics*; D020552:Comet Assay / Q000379:methods*; D004249:DNA Damage; D004058:Diffusion; D006863:Hydrogen-Ion Concentration; D018384:Oxidative Stress / Q000235:genetics