title: Simultaneous detection of Giardia lamblia and Cryptosporidium parvum (oo)cysts in soil using immunomagnetic separation and direct fluorescent antibody staining.
abstract: An improved approach for simultaneous detection of Cryptosporidium parvum and Giardia lamblia (oo)cysts in soil is described. Recoveries>70% were obtained for concentrations>55 and 21 (oo)cysts g(-1) for C. parvum and G. lamblia, respectively. The limits of detection were determined to be<5 (oo)cysts g(-1) soil.
authors.affiliation: Zuckerberg Institute for Water Research, J. Blaustein Institutes for Desert Research, Ben-Gurion University, Midreshet Ben Gurion, 84990 Israel.
authors.forename: Ezra
authors.initials: E
authors.lastname: Orlofsky
authors.forename: Osnat
authors.initials: O
authors.lastname: Gillor
authors.forename: Ann
authors.initials: A
authors.lastname: Melli
authors.forename: Woutrina
authors.initials: W
authors.lastname: Miller
authors.forename: Stefan
authors.initials: S
authors.lastname: Wuertz
authors.forename: Nirit
authors.initials: N
authors.lastname: Bernstein
authors.forename: Karen
authors.initials: K
authors.lastname: Shapiro
mesh_terms: D016785:Cryptosporidium parvum / Q000276:immunology / Q000302:isolation & purification*; D005455:Fluorescent Antibody Technique / Q000379:methods*; D016829:Giardia lamblia / Q000276:immunology / Q000302:isolation & purification*; D018189:Immunomagnetic Separation / Q000379:methods*; D057230:Limit of Detection; D033661:Oocysts; D012987:Soil / Q000469:parasitology*
chemical_list: D012987:Soil
keywords: Cryptosporidium parvum; Detection; Giardia lamblia; Immunomagnetic separation; Recovery; Soil contamination