title: Growth-suppressive effect of the α-amylase inhibitor of Triticum aestivum on stored-product mites varies by the species and type of diet.
abstract: A naturally occurring α-amylase inhibitor (α-AI) of Triticum aestivum protects wheat grain from gramnivorous arthropod pests. The α-AI (Type-I) was incorporated into carbohydrate and protein diets to test its inhibitory activity on the stored-product mites Acarus siro, Lepidoglyphus destructor and Tyrophagus putrescentiae (Acari: Astigmata). Growth tests of mites fed the various diets were used to compare the suppressive effects. The final population size of mites attained from an initial population of 50 specimens maintained under controlled conditions (85 % relative humidity and 25 °C) was compared after 21 days of cultivation. The results showed that α-AI in the concentration in the range of 0.01-1 mg g(-1) did not suppress the growth of the tested stored-product mites. α-AI at a concentration of 10 mg g(-1) exerted a growth-suppressive effect that depended on the diet and species of the mites. The growth rate of A. siro was affected by the type of diet and was higher on carbohydrate diet than on the protein diet, the suppressive effect of α-AI was on the both diets. The growth-suppressive effect of α-AI on L. destructor and T. putrescentiae was significant when they were fed the protein diet but not when they were fed the carbohydrate diet. The higher resistance of tested mites to α-AI (proteinaceous) compared to non-proteinaceous acarbose corresponds to a powerful proteotolytic system in the mite gut. The results are discussed in terms of the adaptability of mites to utilize the starch from food sources.
authors.affiliation: Crop Research Institute, Drnovska 507/73, Ruzyne, Prague 6, 16106, Czech Republic, hubert@vurv.cz.
authors.forename: Jan
authors.initials: J
authors.lastname: Hubert
authors.forename: Marta
authors.initials: M
authors.lastname: Nesvorna
authors.forename: Tomas
authors.initials: T
authors.lastname: Erban
mesh_terms: D000818:Animals; D004032:Diet; D008925:Mites / Q000187:drug effects* / Q000254:growth & development; D010940:Plant Proteins / Q000633:toxicity*; D013045:Species Specificity
chemical_list: D010940:Plant Proteins; C060586:WDAI-3 protein, Triticum aestivum
references.citation: Arch Insect Biochem Physiol. 2010 Nov;75(3):187-206
references.pmid: 20936642
references.citation: Annu Rev Plant Biol. 2003;54:207-33
references.pmid: 14502990
references.citation: Arch Insect Biochem Physiol. 2009 Jul;71(3):139-58
references.pmid: 19480003
references.citation: Eur J Biochem. 2002 Jan;269(2):397-412
references.pmid: 11856298
references.citation: Exp Appl Acarol. 2008 Mar;44(3):199-212
references.pmid: 18357505
references.citation: BMC Biochem. 2012 Jan 31;13:3
references.pmid: 22292590
references.citation: J Med Entomol. 2006 Nov;43(6):1200-7
references.pmid: 17162954
references.citation: Exp Appl Acarol. 2008 Mar;44(3):213-26
references.pmid: 18379887
references.citation: J Econ Entomol. 2005 Jun;98(3):1058-69
references.pmid: 16022339
references.citation: Allergy. 1996 Mar;51(3):176-80
references.pmid: 8781672
references.citation: Exp Appl Acarol. 2013 Jun;60(2):241-52
references.pmid: 23192331
references.citation: Arch Insect Biochem Physiol. 2011 Oct;78(2):74-86
references.pmid: 21818771
references.citation: Exp Appl Acarol. 2012 Mar;56(3):191-208
references.pmid: 22270112
references.citation: Exp Appl Acarol. 2001;25(3):203-15
references.pmid: 11523916
references.citation: J Econ Entomol. 2006 Dec;99(6):2146-50
references.pmid: 17195686
references.citation: Arch Insect Biochem Physiol. 2000 Jun;44(2):69-81
references.pmid: 10861867
references.citation: Clin Exp Allergy. 1997 Aug;27(8):921-5
references.pmid: 9291290
references.citation: J Insect Sci. 2010;10:42
references.pmid: 20572792
references.citation: FEBS J. 2009 Jul;276(13):3531-46
references.pmid: 19476481
references.citation: Phytochemistry. 2005 Jan;66(1):31-9
references.pmid: 15649508
references.citation: J Econ Entomol. 2011 Oct;104(5):1752-64
references.pmid: 22066207
references.citation: Exp Appl Acarol. 2005;35(4):281-91
references.pmid: 15969461
references.citation: Exp Appl Acarol. 1992 Nov;16(1-2):165-80
references.pmid: 1283568
references.citation: Insect Biochem Mol Biol. 1996 May;26(5):419-26
references.pmid: 8763161