title: Expression at a 20L scale and purification of the extracellular domain of the Schistosoma mansoni TSP-2 recombinant protein: a vaccine candidate for human intestinal schistosomiasis.
abstract: A novel recombinant protein vaccine for human schistosomiasis caused by Schistosoma mansoni is under development. The Sm-TSP-2 schistosomiasis vaccine is comprised of a 9 kDa recombinant protein corresponding to the extracellular domain of a unique S. mansoni tetraspanin. Here, we describe the cloning and the expression of the external loop of Sm-TSP-2 recombinant protein secreted by Pichia Pink the process development at 20L scale fermentation, and the two-steps purification, which resulted in a protein recovery yield of 31% and a protein purity of 97%. The developed processes are suitable for the production of purified protein for subsequent formulation and Phase 1 clinical studies.
authors.affiliation: Departments of Pediatrics and Molecular Virology and Microbiology; National School of Tropical Medicine; Baylor College of Medicine; Houston, TX USA; Sabin Vaccine Institute and Texas Children's Hospital Center for Vaccine Development; Houston, TX USA.
authors.forename: Elena
authors.initials: E
authors.lastname: Curti
authors.forename: Clifford
authors.initials: C
authors.lastname: Kwityn
authors.forename: Bin
authors.initials: B
authors.lastname: Zhan
authors.forename: Portia
authors.initials: P
authors.lastname: Gillespie
authors.forename: Jill
authors.initials: J
authors.lastname: Brelsford
authors.forename: Vehid
authors.initials: V
authors.lastname: Deumic
authors.forename: Jordan
authors.initials: J
authors.lastname: Plieskatt
authors.forename: Wanderson C
authors.initials: WC
authors.lastname: Rezende
authors.forename: Eric
authors.initials: E
authors.lastname: Tsao
authors.forename: Bose
authors.initials: B
authors.lastname: Kalampanayil
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Hotez
authors.forename: Maria Elena
authors.initials: ME
authors.lastname: Bottazzi
mesh_terms: D000818:Animals; D000947:Antigens, Helminth / Q000096:biosynthesis* / Q000235:genetics / Q000302:isolation & purification*; D001709:Biotechnology / Q000379:methods; D015870:Gene Expression; D015801:Helminth Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000302:isolation & purification*; D010843:Pichia / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics / Q000302:isolation & purification; D012550:Schistosoma mansoni / Q000235:genetics; D012555:Schistosomiasis mansoni / Q000276:immunology / Q000517:prevention & control*; D013678:Technology, Pharmaceutical / Q000379:methods; D059470:Tetraspanins / Q000096:biosynthesis* / Q000235:genetics / Q000302:isolation & purification*; D014612:Vaccines / Q000096:biosynthesis* / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D000947:Antigens, Helminth; D015801:Helminth Proteins; D011994:Recombinant Proteins; C000589037:TSP-2 protein, Schistosoma mansoni; D059470:Tetraspanins; D014612:Vaccines
keywords: Pichia Pastoris; Schistosoma mansoni; Schistosomiasis; Sm-TSP-2; neglected tropical diseases; recombinant protein; vaccine
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