title: Biophysical characterization of glycodendrimers as nano-carriers for HIV peptides.
abstract: This paper examines the formation and stability of nano-complexes that could provide a new therapeutic approach against HIV-1 infection. Poly(propylene imine) glycodendrimers decorated with 2(nd) generation cationic carbosilane dendrons were generated and their use in polyplex formation checked. Owing to their positively-charged terminal amino groups the hybrid glycodendrimers can bind anionic peptides. It was shown that they form nano-complexes with the HIV-derived peptides P24, Gp160 and Nef. Complexes 130-190 nm in size were formed in molar ratios (dendrimer/ peptide) of (3-4):1. These were sufficiently stable over time and at different pHs. The results obtained suggest that the hybrid dendrimers studied can be considered as alternative carriers for delivering HIV peptides to dendritic cells.
authors.affiliation: Department of General Biophysics, University of Lodz, Pomorska st. 141/143, 90-236, Lodz, Poland.. maksion@biol.uni.lodz.pl.
authors.forename: M
authors.initials: M
authors.lastname: Ionov
authors.forename: K
authors.initials: K
authors.lastname: Ciepluch
authors.forename: B R
authors.initials: BR
authors.lastname: Moreno
authors.forename: D
authors.initials: D
authors.lastname: Appelhans
authors.forename: J
authors.initials: J
authors.lastname: Sánchez-Nieves
authors.forename: R
authors.initials: R
authors.lastname: Gómez
authors.forename: F J
authors.initials: FJ
authors.lastname: de la Mata
authors.forename: M A
authors.initials: MA
authors.lastname: Muñoz-Fernández
authors.forename: M
authors.initials: M
authors.lastname: Bryszewska
mesh_terms: D050091:Dendrimers / Q000737:chemistry*; D004337:Drug Carriers / Q000737:chemistry*; D016655:HIV Core Protein p24 / Q000737:chemistry; D019302:HIV Envelope Protein gp160 / Q000737:chemistry; D015497:HIV-1; D053758:Nanoparticles / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry*; D011126:Polypropylenes / Q000737:chemistry; D012821:Silanes / Q000737:chemistry; D054311:nef Gene Products, Human Immunodeficiency Virus / Q000737:chemistry
chemical_list: D050091:Dendrimers; D004337:Drug Carriers; D016655:HIV Core Protein p24; D019302:HIV Envelope Protein gp160; D010455:Peptides; D011126:Polypropylenes; D012821:Silanes; C504072:carbosilane; D054311:nef Gene Products, Human Immunodeficiency Virus; C443641:poly(propyleneimine)