title: Gastric cancer detection using gastric juice pepsinogen and melanoma-associated gene RNA.
abstract: OBJECTIVE
  To develop a new method for gastric cancer detection with gastric juice using melanoma-associated gene (MAGE) RNA and pepsinogen (PG).
  
  
  METHODS
  In total, 183 gastric juice and paired serum specimens were obtained from 134 patients with gastric cancer and 49 healthy individuals. The gastric juice specimens were analyzed with MAGE A1 to A6 nested reverse transcription-polymerase chain reaction. The serum and gastric juice PG were measured with a PG I and II immunoassay.
  
  
  RESULTS
  The gastric juice PG I and PG I/II ratios were more accurate than those of serum. The combination test using the gastric PG I/II ratio and MAGE was the most accurate, with a sensitivity of 77.6% and a specificity of 87.8%. The sensitivity was 78.8% for stage I gastric cancer and not influenced by cancer location or pathologic type.
  
  
  CONCLUSIONS
  The combination test is potentially an additional tool for gastric cancer detection.
authors.affiliation: Department of Surgery, School of Medicine, Catholic University of Daegu, Daegu, South Korea.
authors.forename: Hyun-Dong
authors.initials: HD
authors.lastname: Chae
authors.forename: In-Ho
authors.initials: IH
authors.lastname: Kim
authors.forename: Geon Ho
authors.initials: GH
authors.lastname: Lee
authors.forename: Im-Hee
authors.initials: IH
authors.lastname: Shin
authors.forename: Hun-Suk
authors.initials: HS
authors.lastname: Suh
authors.forename: Chang-Ho
authors.initials: CH
authors.lastname: Jeon
mesh_terms: D000328:Adult; D000368:Aged; D005260:Female; D005750:Gastric Juice / Q000737:chemistry*; D006801:Humans; D008297:Male; D058950:Melanoma-Specific Antigens / Q000235:genetics*; D008875:Middle Aged; D020116:Pepsinogen A / Q000032:analysis*; D012333:RNA, Messenger; D013274:Stomach Neoplasms / Q000175:diagnosis* / Q000235:genetics
chemical_list: D058950:Melanoma-Specific Antigens; D012333:RNA, Messenger; D020116:Pepsinogen A
keywords: Gastric juice; MAGE; Pepsinogen; Stomach neoplasm