title: Functional properties and expression quantitative trait loci for phosphate transporter GmPT1 in soybean.
abstract: Phosphate (Pi) remobilization within a plant is critical for plant survival under Pi-limiting conditions. In this paper, a soybean Pi transporter gene, GmPT1, was characterized. A marked induction of GmPT1 transcript was observed in young leaves, mature leaves and lateral roots during long-term Pi starvation. Transgenic tobacco plants containing the GmPT1 gene were obtained using an Agrobacterium-mediated transformation system. Compared with wild-type plants, transgenic plants showed significant increases in phosphorus-use efficiency (PUE), photosystem II (PSII) function, total dry weight and seed weight under Pi-deficient conditions. GmPT1 expression levels and PUE were determined in a soybean recombinant inbred line population during a pot experiment that was conducted to measure chlorophyll fluorescence parameters, photosynthetic rate (PN ) and seed yield. Correlation analysis revealed that GmPT1 expression levels had significantly positive correlations with seed yield, PUE, PN and the quantum yield of PSII primary photochemistry (ΦPSII ). Expression quantitative trait loci (eQTL) mapping for GmPT1 revealed two eQTLs, one of which coincided with both the physical location of GmPT1 and a QTL associated with seed yield. These results suggest that GmPT1 plays a role in Pi remobilization, and it may be possible to improve soybean seed yields under Pi-limiting conditions by modulating GmPT1 expression levels.
authors.affiliation: National Center for Soybean Improvement, National Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, 210095, China.
authors.forename: Haina
authors.initials: H
authors.lastname: Song
authors.forename: Zhitong
authors.initials: Z
authors.lastname: Yin
authors.forename: Maoni
authors.initials: M
authors.lastname: Chao
authors.forename: Lihua
authors.initials: L
authors.lastname: Ning
authors.forename: Dan
authors.initials: D
authors.lastname: Zhang
authors.forename: Deyue
authors.initials: D
authors.lastname: Yu
mesh_terms: D002874:Chromosome Mapping; D014644:Genetic Variation; D008969:Molecular Sequence Data; D028061:Phosphate Transport Proteins / Q000235:genetics*; D045332:Photosystem II Protein Complex / Q000502:physiology; D010940:Plant Proteins / Q000235:genetics*; D030821:Plants, Genetically Modified / Q000378:metabolism; D040641:Quantitative Trait Loci; D012333:RNA, Messenger / Q000378:metabolism; D013025:Glycine max / Q000235:genetics*; D014026:Nicotiana / Q000235:genetics
chemical_list: D028061:Phosphate Transport Proteins; D045332:Photosystem II Protein Complex; D010940:Plant Proteins; D012333:RNA, Messenger
keywords: Glycine max (L.) Merr.; chlorophyll fluorescence parameters; phosphate remobilization; seed yield.