title: A novel approach to stimulate the biphenyl-degrading potential of bacterial community from PCBs-contaminated soil of e-waste recycling sites.
abstract: SRpf, culture supernatants from Micrococcus luteus containing the resuscitation-promoting factor (Rpf), was used to enhance the biphenyl-degrading capability of potential microorganisms. The obtained results suggest that the enrichment culture produced by the addition of SRpf (enrichment culture treatment group, ECT) enhanced the biphenyl degradation efficiency, cell growth and bacterial diversity significantly. Biphenyl at concentration of 1500 mg/L was almost completely degraded in 24 h using SRpf at a dosage of 15% (v/v). Six strains unique to the ECT were isolated in pure cultures. This study provides a new insight into bacterial degradation of biphenyl for PCBs-bioremediation, and could be developed as a novel efficient method for obtaining highly desirable pollutant-degrading microorganisms.
authors.affiliation: Department of Environmental Engineering, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310058, China.
authors.forename: Xiaomei
authors.initials: X
authors.lastname: Su
authors.affiliation: Department of Environmental Engineering, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310058, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Shen
authors.affiliation: Department of Environmental Engineering, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310058, China.
authors.forename: Xioyan
authors.initials: X
authors.lastname: Yao
authors.affiliation: College of Geography and Environmental Science, Zhejiang Normal University, Jinhua 321004, China.
authors.forename: Linxian
authors.initials: L
authors.lastname: Ding
authors.affiliation: Center for Biomedicine and Health, Hangzhou Normal University, Hangzhou 311121, China.
authors.forename: Chunna
authors.initials: C
authors.lastname: Yu
authors.affiliation: Department of Environmental Engineering, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310058, China. Electronic address: ysxzt@zju.edu.cn.
authors.forename: Chaofeng
authors.initials: C
authors.lastname: Shen
mesh_terms: D042402:Achromobacter / Q000378:metabolism; D000421:Alcaligenes / Q000378:metabolism; D001173:Arthrobacter / Q000378:metabolism; D001673:Biodegradation, Environmental; D001713:Biphenyl Compounds / Q000737:chemistry*; D045247:Chryseobacterium / Q000378:metabolism; D003247:Conservation of Natural Resources; D058645:Denaturing Gradient Gel Electrophoresis; D059029:Electronic Waste; D004785:Environmental Pollutants / Q000378:metabolism; D016982:Micrococcus luteus / Q000378:metabolism*; D010802:Phylogeny; D011078:Polychlorinated Biphenyls / Q000032:analysis*; D016133:Polymerase Chain Reaction; D059027:Recycling; D012240:Rhodococcus / Q000378:metabolism; D012987:Soil; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000737:chemistry*; D013997:Time Factors
chemical_list: D001713:Biphenyl Compounds; D004785:Environmental Pollutants; D011078:Polychlorinated Biphenyls; D012987:Soil; D012989:Soil Pollutants; C010574:biphenyl
keywords: Biphenyl degradation; Mixed-culture; Polychlorinated biphenyls; Resuscitation-promoting factor; Uncultured bacteria