title: [Genetic characteristics of viral quasispecies of HIV-1 CRF07_BC among intravenous drug users].
abstract: To explore the genetic characteristics of viral quasispecies in HIV-1 CRF07_BC infections among intravenous drug users (IDU), the gp120 fragments of HIV-1 env gene were amplified from plasma samples collected from 6 CRF07_BC infected persons using single genome amplification and sequencing (SGA/ SGS) method, and 11 to 28 sequences were obtained from these samples, respectively, A neighbor-joining phylogenetic tree was reconstructed to describe the genetic characteristics of viral quasispecies. The Simplot, segments' phylogenetic trees and diversity plots based on average pairwise distance (APD) were used to identify the recombination events between quasispecies. The SGA sequences derived from single specimen formed a large monophyletic cluster in the neighbor-joining phylogenetic tree and showed the complex topologic structures of viral quasispecies. Of the 6 CRF07_BC infected patients, only one possessed the high genetic homogeneity, whereas the other five individuals showed high heterogeneity, with two to four subclusters inside the monophyletic cluster for each specimen. In addition, the recombinant events were identified among viral quasispecies from 3 cases. The results show SGA technique and phylogenetic analyses are useful tool to investigate the intrahost CRF07_BC gp120 complex quasispecies variation and high genetic diversity.
authors.affiliation: Beijing Centers for Preventive Medical Research, Beijing 100013, China. ruoleixin@gmail.com
authors.forename: Ruo-Lei
authors.initials: RL
authors.lastname: Xin
authors.forename: Ze-Qin
authors.initials: ZQ
authors.lastname: Ma
authors.forename: Chun-Lin
authors.initials: CL
authors.lastname: Cheng
authors.forename: Hui
authors.initials: H
authors.lastname: Xing
authors.forename: Kun-Xue
authors.initials: KX
authors.lastname: Hong
authors.forename: Yu-Hua
authors.initials: YH
authors.lastname: Ruan
authors.forename: Jia
authors.initials: J
authors.lastname: Li
authors.forename: Hong-Yan
authors.initials: HY
authors.lastname: Lu
authors.forename: Yi-Ming
authors.initials: YM
authors.lastname: Shao
authors.forename: Xiang
authors.initials: X
authors.lastname: He
mesh_terms: D000328:Adult; D055030:Drug Users; D005260:Female; D015658:HIV Infections / Q000821:virology*; D015497:HIV-1 / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D010802:Phylogeny; D015819:Substance Abuse, Intravenous / Q000821:virology*; D055815:Young Adult