title: DNA methyltransferase inhibitor assay system based on the HBx-induced DNA methylation of E-cadherin.
abstract: We here report a simple assay system for DNA methyltransferase (DNMT) inhibitors based on the HBx-induced DNA methylation of E-cadherin. A stable cell line named G1 was generated by co-transfecting E-cadherin luciferase reporter and HBx-expression plasmid into HepG2 cells. Treatment of G1 cells with DNMT inhibitors, 5-azacytidine, 5-aza-2'-deoxycytidine, and procainamaid, dose-dependently inhibited DNA methylation of E-cadherin promoter in the reporter, resulting in up-regulation of luciferase levels and its enzyme activity. Treatment with all-trans retinoic acid that is known to inhibit DNMT expression, also induced similar effects. Our system can be useful for development of epi-drugs targeting DNA methylation in malignancies.
authors.affiliation: Department of Microbiology, College of Natural Sciences, Pusan National University, Busan 609-735, Republic of Korea.
authors.forename: Hyehyeon
authors.initials: H
authors.lastname: Lee
authors.forename: Jin Bae
authors.initials: JB
authors.lastname: Sohn
authors.forename: Soo Shin
authors.initials: SS
authors.lastname: Kim
authors.forename: Kyung Lib
authors.initials: KL
authors.lastname: Jang
mesh_terms: D015820:Cadherins / Q000096:biosynthesis / Q000378:metabolism*; D004248:DNA (Cytosine-5-)-Methyltransferases / Q000037:antagonists & inhibitors*; D019175:DNA Methylation / Q000187:drug effects*; D015536:Down-Regulation; D004791:Enzyme Inhibitors / Q000032:analysis*; D056945:Hep G2 Cells; D006801:Humans; D008156:Luciferases / Q000235:genetics; D011401:Promoter Regions, Genetic; D015534:Trans-Activators / Q000378:metabolism*; D014162:Transfection; D054334:Viral Regulatory and Accessory Proteins
chemical_list: D015820:Cadherins; D004791:Enzyme Inhibitors; D015534:Trans-Activators; D054334:Viral Regulatory and Accessory Proteins; C057628:hepatitis B virus X protein; D008156:Luciferases; D004248:DNA (Cytosine-5-)-Methyltransferases
keywords: DNA methylation; DNA methyltransferase; E-cadherin; Epi-drug; HBx