title: Enterocin T, a novel class IIa bacteriocin produced by Enterococcus sp. 812.
abstract: Enterococcus sp. 812, isolated from fresh broccoli, was previously found to produce a bacteriocin active against a number of Gram-positive bacteria, including Listeria monocytogenes. Bacteriocin activity decreased slightly after autoclaving (121 °C for 15 min), but was inactivated by protease K. Mass spectrometry analysis revealed the bacteriocin mass to be approximately 4,521.34 Da. N-terminal amino acid sequencing yielded a partial sequence, NH2-ATYYGNGVYXDKKKXWVEWGQA, by Edman degradation, which contained the consensus class IIa bacteriocin motif YGNGV in the N-terminal region. The obtained partial sequence showed high homology with some enterococcal bacteriocins; however, no identical peptide or protein was found. This peptide was therefore considered to be a novel bacteriocin produced by Enterococcus sp. 812 and was termed enterocin T.
authors.affiliation: Department of Biotechnology, Ming Chuan University, No. 5 De-Ming Rd., Gui-Shan Township, Taoyuan, 333, Taiwan. yisheng@mail.mcu.edu.tw
authors.forename: Yi-Sheng
authors.initials: YS
authors.lastname: Chen
authors.forename: Chi-Rong
authors.initials: CR
authors.lastname: Yu
authors.forename: Si-Hua
authors.initials: SH
authors.lastname: Ji
authors.forename: Min-Shiuan
authors.initials: MS
authors.lastname: Liou
authors.forename: Kun-Hon
authors.initials: KH
authors.lastname: Leong
authors.forename: Shwu-Fen
authors.initials: SF
authors.lastname: Pan
authors.forename: Hui-Chung
authors.initials: HC
authors.lastname: Wu
authors.forename: Yu-Hsuan
authors.initials: YH
authors.lastname: Lin
authors.forename: Bi
authors.initials: B
authors.lastname: Yu
authors.forename: Fujitoshi
authors.initials: F
authors.lastname: Yanagida
mesh_terms: D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D001430:Bacteriocins / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism*; D016983:Enterococcus / Q000737:chemistry / Q000378:metabolism; D006094:Gram-Positive Bacteria; D016415:Sequence Alignment
chemical_list: D001430:Bacteriocins; C584148:enterocin T, Enterococcus