title: Free fatty acid secretion by an engineered strain of Escherichia coli.
abstract: Although most bacteria produce fatty acids (FA), few secrete free FAs into the culture media. Over-expression of two FA thioesterases, TesA and AtFatA, facilitated both total and FFA production in a recombinant strain of Escherichia coli. When these thioesterases were expressed in a fadD and fadL double-deletion strain, a further enhancement of FFA secretion was observed. These results support a simple diffusion mechanism for FA transport. In addition, the ATP-binding cassette transporter protein, MsbA, also increased the concentration of FFAs in the culture. The final strain produced 110 mg FFA/l, about 33 % of the total FAs being produced. Our findings support a diffusion mechanism for FA transport.
authors.affiliation: College of Food Science and Engineering, Liaoning Medical University, Jinzhou, 121000, China, woxing1981@163.com.
authors.forename: Xin
authors.initials: X
authors.lastname: Meng
authors.forename: Hongli
authors.initials: H
authors.lastname: Shang
authors.forename: Yi
authors.initials: Y
authors.lastname: Zheng
authors.forename: Zhen
authors.initials: Z
authors.lastname: Zhang
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000378:metabolism; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics; D001426:Bacterial Proteins / Q000378:metabolism; D056804:Biofuels; D003066:Coenzyme A Ligases / Q000235:genetics; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000235:genetics; D050612:Fatty Acid Transport Proteins / Q000235:genetics; D005230:Fatty Acids, Nonesterified / Q000032:analysis / Q000378:metabolism*; D055786:Gene Knockout Techniques; D060847:Metabolic Engineering / Q000379:methods*
chemical_list: D018528:ATP-Binding Cassette Transporters; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D056804:Biofuels; D029968:Escherichia coli Proteins; D050612:Fatty Acid Transport Proteins; D005230:Fatty Acids, Nonesterified; C079726:MsbA protein, Bacteria; C065691:fadL protein, E coli; D003066:Coenzyme A Ligases; C085934:acyl-coenzyme A synthetase, E coli