title: Non-direct sequence ions in the tandem mass spectrometry of protonated peptide amides--an energy-resolved study.
abstract: The fragmentation reactions of the MH(+) ions of Leu-enkephalin amide and a variety of heptapeptide amides have been studied in detail as a function of collision energy using a QqToF beam type mass spectrometer. The initial fragmentation of the protonated amides involves primarily formation of bn ions, including significant loss of NH3 from the MH(+) ions. Further fragmentation of these bn ions occurs following macrocyclization/ring opening leading in many cases to bn ions with permuted sequences and, thus, to formation of non-direct sequence ions. The importance of these non-direct sequence ions increases markedly with increasing collision energy, making peptide sequence determination difficult, if not impossible, at higher collision energies.
authors.affiliation: Department of Chemistry, University of Toronto, Toronto, ON, M5S 3H6, Canada, AHARRISO@CHEM.UTORONTO.CA.
authors.forename: Alex G
authors.initials: AG
authors.lastname: Harrison
authors.forename: Cagdas
authors.initials: C
authors.lastname: Tasoglu
authors.forename: Talat
authors.initials: T
authors.lastname: Yalcin
mesh_terms: D000577:Amides / Q000737:chemistry*; D007477:Ions / Q000737:chemistry; D008970:Molecular Weight; D009842:Oligopeptides / Q000737:chemistry*; D011522:Protons; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D000577:Amides; D007477:Ions; D009842:Oligopeptides; D011522:Protons
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