title: The efficiency of combined CaO/electrochemical treatment in removal of acid mine drainage induced toxicity and genotoxicity.
abstract: Acid mine drainage (AMD) is a by-product of the mining industry that has a detrimental effect on aquatic plant and animal life due to high load of heavy metals and sulfates. In the present study, the toxic and genotoxic potential of AMD prior to and following combination of neutralization/electrocoagulation processes was evaluated using several bioassays and selected parameters. Regardless of pH correction of AMD prior to Daphnia bioassay, high acute toxicity was observed in Daphnia magna. The mine leachate also induced strong phyto-, cyto- and genotoxicity to Allium cepa roots. Short term exposure to AMD inhibited duckweed growth and chlorophyll a content and simultaneously promoted lipid peroxidation and DNA damage despite duckweed capability to upregulate antioxidative defense mechanisms. The results show that observed (geno)toxicity could be related to oxidative stress most probably induced by toxic metal action. However, influence of low pH as a contributing factor in the phytotoxicity of AMD cannot be excluded. The application of combined treatment eliminated genotoxicity and was highly efficient in reducing toxicity of AMD. Thus, the method seems to be suitable for treatment of AMD waters enabling their safe discharge to an aquatic environment.
authors.affiliation: Department of Biology, Faculty of Science, University of Zagreb, Rooseveltov trg 6/III, HR-10000, Zagreb, Croatia. Electronic address: sandra@biol.pmf.hr.
authors.forename: Sandra
authors.initials: S
authors.lastname: Radić
authors.forename: Valerija
authors.initials: V
authors.lastname: Vujčić
authors.forename: Želimira
authors.initials: Ž
authors.lastname: Cvetković
authors.forename: Petra
authors.initials: P
authors.lastname: Cvjetko
authors.forename: Višnja
authors.initials: V
authors.lastname: Oreščanin
mesh_terms: D000490:Allium / Q000187:drug effects; D000818:Animals; D029064:Araceae / Q000187:drug effects; D001681:Biological Assay; D017610:Calcium Compounds / Q000737:chemistry*; D003621:Daphnia / Q000187:drug effects; D055664:Electrochemical Techniques / Q000379:methods*; D052918:Environmental Restoration and Remediation / Q000379:methods*; D018384:Oxidative Stress / Q000187:drug effects; D010087:Oxides / Q000737:chemistry*; D055771:Serbia; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D017610:Calcium Compounds; D010087:Oxides; D014874:Water Pollutants, Chemical; C016538:lime
keywords: (Geno)toxicity; Biochemical indicators; Daphnia; Plants; Water treatment