title: Dynamics of the shade-avoidance response in Arabidopsis.
abstract: Shade-intolerant plants perceive the reduction in the ratio of red light (R) to far-red light (FR) as a warning of competition with neighboring vegetation and display a suite of developmental responses known as shade avoidance. In recent years, major progress has been made in understanding the molecular mechanisms underlying shade avoidance. Despite this, little is known about the dynamics of this response and the cascade of molecular events leading to plant adaptation to a low-R/FR environment. By combining genome-wide expression profiling and computational analyses, we show highly significant overlap between shade avoidance and deetiolation transcript profiles in Arabidopsis (Arabidopsis thaliana). The direction of the response was dissimilar at the early stages of shade avoidance and congruent at the late ones. This latter regulation requires LONG HYPOCOTYL IN FAR RED1/SLENDER IN CANOPY SHADE1 and phytochrome A, which function largely independently to negatively control shade avoidance. Gene network analysis highlights a subnetwork containing ELONGATED HYPOCOTYL5 (HY5), a master regulator of deetiolation, in the wild type and not in phytochrome A mutant upon prolonged low R/FR. Network analysis also highlights a direct connection between HY5 and HY5 HOMOLOG (HYH), a gene functionally implicated in the inhibition of hypocotyl elongation and known to be a direct target of the HY5 transcription factor. Kinetics analysis show that the HYH gene is indeed late induced by low R/FR and that its up-regulation depends on the action of HY5, since it does not occur in hy5 mutant. Therefore, we propose that one way plants adapt to a low-R/FR environment is by enhancing HY5 function.
authors.affiliation: Institute of Molecular Biology and Pathology, National Research Council, 00185 Rome, Italy.
authors.forename: Andrea
authors.initials: A
authors.lastname: Ciolfi
authors.forename: Giovanna
authors.initials: G
authors.lastname: Sessa
authors.forename: Massimiliano
authors.initials: M
authors.lastname: Sassi
authors.forename: Marco
authors.initials: M
authors.lastname: Possenti
authors.forename: Samanta
authors.initials: S
authors.lastname: Salvucci
authors.forename: Monica
authors.initials: M
authors.lastname: Carabelli
authors.forename: Giorgio
authors.initials: G
authors.lastname: Morelli
authors.forename: Ida
authors.initials: I
authors.lastname: Ruberti
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000502:physiology / Q000528:radiation effects*; D029681:Arabidopsis Proteins / Q000235:genetics*; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D053263:Gene Regulatory Networks; D018745:Genome, Plant; D008027:Light; D055537:Light Signal Transduction; D020411:Oligonucleotide Array Sequence Analysis
chemical_list: D029681:Arabidopsis Proteins
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