title: Ethyl-pyruvate reduces lung injury matrix metalloproteinases and cytokines and improves survival in experimental model of severe acute pancreatitis.
abstract: OBJECTIVE
  To investigate if the ethyl-pyruvate solution could reduce mortality in AP and/or diminish the acute lung injury.
  
  
  METHODS
  Forty male rats, weighing between 270 to 330 grams were operated. An experimental model of severe AP by injection of 0.1 ml/100g of 2.5% sodium taurocholate into the bilio-pancreatic duct was utilized. The rats were divided into two groups of ten animals each: CT - control (treatment with 50 ml/kg of Ringer's solution, intraperitoneal) and EP (treatment with 50 ml/kg of Ringer ethyl-pyruvate solution, intra-peritoneal), three hours following AP induction. After six hours, a new infusion of the treatment solution was performed in each group. Two hours later, the animals were killed and the pulmonary parenchyma was resected for biomolecular analysis, consisting of: interleukin, myeloperoxidase, MDA, nitric oxide, metalloproteinases and heat shock protein. In the second part of the experiment, another, 20 rats were randomly divided into EP and CT groups, in order to evaluate a survival comparison between the two groups.
  
  
  RESULTS
  There were no significant differences in IL-1B,IL-10, MMP-9, HSP70, nitric oxide, MPO, MDA (lipidic peroxidation) concerning both groups. The levels of IL-6 were significantly diminished in the EP group. Furthermore, the MMP-2 levels were also reduced in the EP group (p<0.05). The animals from the EP treatment groups had improved survival, when compared to control group (p<0.05).
  
  
  CONCLUSIONS
  The ethyl-pyruvate diminishes acute lung injury inflammatory response in acute pancreatitis and ameliorates survival when compared to control group, in the experimental model of necrotizing acute pancreatitis.
authors.affiliation: Postgraduate Program in Gastrointestinal Surgery, Federal University of Sao Paulo - Paulista Medical School, Sao Paulo, Brazil. matone@einstein.br
authors.forename: Jacques
authors.initials: J
authors.lastname: Matone
authors.forename: Ana Iochabel Soares
authors.initials: AI
authors.lastname: Moretti
authors.forename: Franz Robert
authors.initials: FR
authors.lastname: Apodaca-Torrez
authors.forename: Alberto
authors.initials: A
authors.lastname: Goldenberg
mesh_terms: D055371:Acute Lung Injury / Q000139:chemically induced / Q000188:drug therapy* / Q000201:enzymology; D000818:Animals; D016207:Cytokines / Q000378:metabolism*; D004195:Disease Models, Animal; D015151:Immunoblotting; D007552:Isotonic Solutions / Q000494:pharmacology; D053208:Kaplan-Meier Estimate; D008297:Male; D020782:Matrix Metalloproteinases / Q000378:metabolism*; D019283:Pancreatitis, Acute Necrotizing / Q000188:drug therapy* / Q000401:mortality; D011773:Pyruvates / Q000494:pharmacology*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D012016:Reference Values; D015203:Reproducibility of Results; D013997:Time Factors; D016896:Treatment Outcome
chemical_list: D016207:Cytokines; D007552:Isotonic Solutions; D011773:Pyruvates; C439503:Ringer's ethyl pyruvate; C046522:ethyl pyruvate; D020782:Matrix Metalloproteinases