title: Promotion effect of constituents from the root of Polygonum multiflorum on hair growth.
abstract: Two new compounds, gallic acid ester of torachrysone-8-O-β-D-glucoside (1) and (E)-2,3,5,4'-tetrahydroxystilbene-2-O-β-D-xyloside (4), along with eight known compounds (2, 3, 5-10) were isolated from a 70% ethanol extract of Polygonum multiflorum roots. The structures were determined by (1)H and (13)C NMR, HMQC, and HMBC spectrometry. Extracts of P. multiflorum have been reported to promote hair growth in vivo. This study was carried out to evaluate the effects of isolated compounds from P. multiflorum on promoting hair growth using dermal papilla cells (DPCs), which play an important role in hair growth. When DPCs were treated with compounds (1-10) from P. multiflorum, compounds 1, 2, 3, 6, and 10 increased the proliferation of DPCs compared with the control. Specifically, compound 2 (10 and 20 μM) induced a greater increase in the proliferation of DPCs than minoxidil (10 μM). Additionally, treatment of vibrissa follicles with compound 2 for 21 days increased hair-fiber length significantly. On the basis of this result, further investigation and optimization of these derivatives might help in the development of therapeutic agents for the treatment of alopecia.
authors.affiliation: College of Pharmacy, Chungnam National University, Daejeon 305-764, Republic of Korea.
authors.forename: Ya Nan
authors.initials: YN
authors.lastname: Sun
authors.forename: Long
authors.initials: L
authors.lastname: Cui
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.forename: Xi Tao
authors.initials: XT
authors.lastname: Yan
authors.forename: Seo Young
authors.initials: SY
authors.lastname: Yang
authors.forename: Jung Il
authors.initials: JI
authors.lastname: Kang
authors.forename: Hee Kyoung
authors.initials: HK
authors.lastname: Kang
authors.forename: Young Ho
authors.initials: YH
authors.lastname: Kim
mesh_terms: D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D020405:Dermis / Q000166:cytology / Q000187:drug effects; D005960:Glucosides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D006027:Glycosides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D006197:Hair / Q000166:cytology / Q000187:drug effects* / Q000254:growth & development*; D018859:Hair Follicle / Q000166:cytology / Q000187:drug effects / Q000254:growth & development; D006801:Humans; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D018517:Plant Roots / Q000737:chemistry; D028502:Polygonum / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D005960:Glucosides; D006027:Glycosides; D010936:Plant Extracts; C010807:xylosides
keywords: Dermal papilla cells (DPCs); Hair growth; Hair-fiber length; Polygonum multiflorum