title: NAD(P)H: quinone oxidoreductase 1 and NRH:quinone oxidoreductase 2 polymorphisms in papillary thyroid microcarcinoma: correlation with phenotype.
abstract: OBJECTIVE
  NAD(P)H:Quinone Oxidoreductase 1 (NQO1) C609T missense variant (NQO1*2) and 29 basepair (bp)-insertion/deletion (I29/D) polymorphism of the NRH:Quinone Oxidoreductase 2 (NQO2) gene promoter have been proposed as predictive and prognostic factors for cancer development and progression. The purpose of this study is to investigate the relationship between NQO1/NQO2 genotype and clinico-pathological features of papillary thyroid microcarcinoma (PTMC).
  
  
  METHODS
  Genomic DNA was isolated from 243 patients; and clinical data were retrospectively analyzed. NQO1*2 and tri-allelic polymorphism of NQO2 were investigated by polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) analysis.
  
  
  RESULTS
  PTMC with NQO1*2 frequently exhibited extra-thyroidal extension as compared to PTMC with wild-type NQO1 (p=0.039). There was a significant relationship between I29/I29 homozygosity of NQO2 and lymph node metastasis (p=0.042). Multivariate analysis showed that the I29/I29 genotype was associated with an increased risk of lymph node metastasis (OR, 2.24; 95% CI, 1.10-4.56; p=0.026).
  
  
  CONCLUSIONS
  NQO1*2 and I29 allele of the NQO2 are associated with aggressive clinical phenotypes of PTMC, and the I29 allele represents a putative prognostic marker for PTMC.
authors.affiliation: Research Center for Endocrine and Metabolic Diseases, Chungnam National University Hospital, 282 Munhwa-ro, Jung-gu, Daejeon 301-721, Korea.
authors.forename: Junguee
authors.initials: J
authors.lastname: Lee
authors.forename: Koon Soon
authors.initials: KS
authors.lastname: Kim
authors.forename: Min Ho
authors.initials: MH
authors.lastname: Lee
authors.forename: Yeon Soo
authors.initials: YS
authors.lastname: Kim
authors.forename: Min Hee
authors.initials: MH
authors.lastname: Lee
authors.forename: Seong Eun
authors.initials: SE
authors.lastname: Lee
authors.forename: Yong Kyung
authors.initials: YK
authors.lastname: Kim
authors.forename: Min Jeong
authors.initials: MJ
authors.lastname: Ryu
authors.forename: Soung Jung
authors.initials: SJ
authors.lastname: Kim
authors.forename: Min Jeong
authors.initials: MJ
authors.lastname: Choi
authors.forename: Young Suk
authors.initials: YS
authors.lastname: Jo
mesh_terms: D000328:Adult; D002291:Carcinoma, Papillary / Q000235:genetics* / Q000473:pathology; D004252:DNA Mutational Analysis; D005260:Female; D020022:Genetic Predisposition to Disease; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D015999:Multivariate Analysis; D016254:Mutagenesis, Insertional; D020125:Mutation, Missense; D016660:NAD(P)H Dehydrogenase (Quinone) / Q000737:chemistry / Q000235:genetics*; D010641:Phenotype; D011110:Polymorphism, Genetic; D011379:Prognosis; D011401:Promoter Regions, Genetic; D012189:Retrospective Studies; D020539:Sequence Analysis, Protein; D017384:Sequence Deletion; D013964:Thyroid Neoplasms / Q000235:genetics* / Q000473:pathology
chemical_list: D016660:NAD(P)H Dehydrogenase (Quinone); C447479:NQO1 protein, human
keywords: NAD(P)H:Quinone Oxidoreductase 1; NRH:Quinone Oxidoreductase 2; thyroid neoplasm
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