title: MicroRNA-21 promotes the ovarian teratocarcinoma PA1 cell line by sustaining cancer stem/progenitor populations in vitro.
abstract: BACKGROUND
  Resistance of cancer stem/progenitor cells (CSPCs) to chemotherapy can lead to cancer relapse. Ovarian teratocarcinoma (OVTC) arises from germ cells and comprises pluripotent cells that can be used to study cancer cell stemness. In this study, we evaluated whether microRNA-21 (miR-21) promotes ovarian teratocarcinoma by maintaining cancer stem/progenitor populations.
  
  
  METHODS
  The lentiviral delivery system was used to upregulate or to suppress the expression of miR-21 in the human ovarian teratocarcinoma cell line PA1 and cell growth assays were used to monitor the expression of miR-21 at different time points. Antibodies directed toward CD133, a stem cell marker, were used to identify CSPCs in the PA1 cell population, and the level of miR-21 expression was determined in enriched CSPCs. Stem cell functional assays (sphere assay and assays for CD133 expression) were used to assess the effects of miR-21 on progression of the CD133+ population.
  
  
  RESULTS
  Knockdown of miR-21 in PA1 cells attenuated growth of PA1 cells whereas overexpression of miR-21 promoted cell growth. Moreover, knockdown of miR-21 resulted in a marked reduction in the CD133+ population and sphere formation of CSPCs. In contrast, overexpression of miR-21 resulted in a marked increase in the population of CD133+ cells as well as sphere formation of CSPCs.
  
  
  CONCLUSIONS
  MicroRNA-21 plays a significant role in cancer growth by regulating stemness in cancer cells.
authors.forename: Wei-Min
authors.initials: WM
authors.lastname: Chung
authors.forename: Wei-Chun
authors.initials: WC
authors.lastname: Chang
authors.forename: Lumin
authors.initials: L
authors.lastname: Chen
authors.forename: Ying-Yi
authors.initials: YY
authors.lastname: Chang
authors.forename: Chih-Rong
authors.initials: CR
authors.lastname: Shyr
authors.forename: Yao-Ching
authors.initials: YC
authors.lastname: Hung
authors.forename: Wen-Lung
authors.initials: WL
authors.lastname: Ma
mesh_terms: D045744:Cell Line, Tumor; D049109:Cell Proliferation; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D035683:MicroRNAs / Q000378:metabolism*; D014411:Neoplastic Stem Cells / Q000378:metabolism*; D010051:Ovarian Neoplasms; D013234:Stem Cells / Q000378:metabolism*; D018243:Teratocarcinoma; D014162:Transfection
chemical_list: D035683:MicroRNAs
references.citation: Proc Natl Acad Sci U S A. 2006 Jul 25;103(30):11154-9
references.pmid: 16849428
references.citation: J Pediatr Adolesc Gynecol. 2011 Feb;24(1):e1-3
references.pmid: 20869284
references.citation: Oncol Rep. 2011 Jun;25(6):1721-9
references.pmid: 21468550
references.citation: Oncol Lett. 2012 Oct;4(4):672-676
references.pmid: 23205081
references.citation: Biochem Soc Trans. 2009 Aug;37(Pt 4):918-25
references.pmid: 19614619
references.citation: Proc Natl Acad Sci U S A. 2006 Feb 14;103(7):2257-61
references.pmid: 16461460
references.citation: Clin Invest Med. 2011 Oct 01;34(5):E281
references.pmid: 21968270
references.citation: Nat Rev Mol Cell Biol. 2009 Feb;10(2):116-25
references.pmid: 19165214
references.citation: BMC Med Genomics. 2012 May 29;5:18
references.pmid: 22643117
references.citation: Cancer Sci. 2012 Jun;103(6):1058-64
references.pmid: 22435731
references.citation: Anticancer Res. 2012 Jul;32(7):2835-41
references.pmid: 22753745
references.citation: Oncogene. 2009 Jan 15;28(2):209-18
references.pmid: 18836486
references.citation: Histol Histopathol. 2010 Jan;25(1):113-20
references.pmid: 19924647
references.citation: Mol Cell Biochem. 2012 Apr;363(1-2):427-36
references.pmid: 22187223
references.citation: J Cell Physiol. 2012 Jul;227(7):2947-52
references.pmid: 21959795
references.citation: J Biol Chem. 2011 May 27;286(21):19127-37
references.pmid: 21471222
references.citation: Biomed Imaging Interv J. 2005 Oct;1(2):e10
references.pmid: 21625278
references.citation: Hepatology. 2012 Jul;56(1):176-85
references.pmid: 22318717
references.citation: Int J Cancer. 2012 Jan 1;130(1):29-39
references.pmid: 21480217
references.citation: J Mol Cell Biol. 2013 Feb;5(1):14-26
references.pmid: 22831834
references.citation: J Pediatr Surg. 2012 Oct;47(10):1797-805
references.pmid: 23084187
references.citation: BMC Cancer. 2010 Mar 26;10:116
references.pmid: 20346143
references.citation: Oncol Rep. 2011 Jan;25(1):141-6
references.pmid: 21109969
references.citation: Mol Cancer Ther. 2010 Sep;9(9):2450-7
references.pmid: 20716638
references.citation: Exp Mol Pathol. 2011 Oct;91(2):596-602
references.pmid: 21787767
references.citation: Proc Natl Acad Sci U S A. 2009 Sep 22;106(38):16281-6
references.pmid: 19805294
references.citation: Nat Rev Cancer. 2005 Apr;5(4):275-84
references.pmid: 15803154
references.citation: Nature. 2008 May 8;453(7192):223-7
references.pmid: 18362916
references.citation: PLoS One. 2012;7(6):e39520
references.pmid: 22761812
references.citation: J Transl Med. 2006 Jan 24;4:6
references.pmid: 16433903
references.citation: Med Pregl. 2011 Jan-Feb;64(1-2):93-5
references.pmid: 21548277
references.citation: Stem Cells. 2009 Dec;27(12):2875-83
references.pmid: 19816957
references.citation: Cancer Cell. 2011 Nov 15;20(5):635-48
references.pmid: 22094257
references.citation: Cancer Res. 2008 Jun 1;68(11):4311-20
references.pmid: 18519691
references.citation: APMIS. 2011 Oct;119(10):663-73
references.pmid: 21917003
references.citation: Curr Opin Cell Biol. 2008 Apr;20(2):214-21
references.pmid: 18329869
references.citation: Breast Cancer Res. 2011 Jan 10;13(1):R2
references.pmid: 21219636
references.citation: Int J Hematol. 2013 Feb;97(2):223-31
references.pmid: 23275230
references.citation: Int J Mol Med. 2010 Dec;26(6):819-27
references.pmid: 21042775
references.citation: Oncogene. 2010 May 6;29(18):2672-80
references.pmid: 20190812
references.citation: Genes Dev. 2006 Mar 1;20(5):515-24
references.pmid: 16510870
references.citation: Cancer Res. 2005 Apr 15;65(8):3025-9
references.pmid: 15833827
references.citation: Cancer Sci. 2010 Oct;101(10):2179-85
references.pmid: 20804503
references.citation: J Pak Med Assoc. 2009 Jan;59(1):18-21
references.pmid: 19213371
references.citation: World J Stem Cells. 2012 Jul 26;4(7):62-70
references.pmid: 22993663
references.citation: J Exp Clin Cancer Res. 2010 Mar 27;29:29
references.pmid: 20346171
references.citation: Curr Opin Cell Biol. 2009 Jun;21(3):452-60
references.pmid: 19450959
references.citation: Mol Cancer. 2006 Dec 02;5:67
references.pmid: 17140455
references.citation: Pharm Biol. 2012 Oct;50(10):1233-40
references.pmid: 22957792
references.citation: J Cell Mol Med. 2009 Jan;13(1):39-53
references.pmid: 19175699
references.citation: Natl Med J India. 2010 Nov-Dec;23(6):346-50
references.pmid: 21561046
references.citation: Arch Med Res. 2011 May;42(4):281-90
references.pmid: 21820606
references.citation: Hum Reprod. 2004 Aug;19(8):1867-70
references.pmid: 15192066
references.citation: Proc Natl Acad Sci U S A. 2010 Dec 7;107(49):21098-103
references.pmid: 21078976
references.citation: AAPS J. 2009 Dec;11(4):682-92
references.pmid: 19842044
references.citation: Cancer Res. 2013 Jan 1;73(1):406-16
references.pmid: 23135908