title: High through-put and highly sensitive liquid chromatography-tandem mass spectrometry separations of essential amino acids using active flow technology chromatography columns.
abstract: A preliminary investigation was undertaken to assess the performance of a new chromatography column technology in applications involving liquid chromatography coupled to mass spectrometry. The new column design allows mobile phase and solute to be extracted from the radial central region of the column, which reduces the solvent load to the mass spectrometer and improves separation efficiency. Effectively the column functions as a 'wall-less' column. The advantages of this design is that the analysis through-put can be increased by a factor of five, while at the same time there is a reduction in baseline noise, which results in an increase in the signal to noise response by up to 10-fold in comparison to standard columns with the same internal diameter and approaching 66-fold in comparison to standard columns with the same virtual internal diameter.
authors.affiliation: Australian Centre for Research on Separation Science (ACROSS), School of Science and Health, University of Western Sydney (Parramatta), Sydney, NSW, Australia.
authors.forename: D
authors.initials: D
authors.lastname: Kocic
authors.forename: L
authors.initials: L
authors.lastname: Pereira
authors.forename: D
authors.initials: D
authors.lastname: Foley
authors.forename: T
authors.initials: T
authors.lastname: Edge
authors.forename: J A
authors.initials: JA
authors.lastname: Mosely
authors.forename: H
authors.initials: H
authors.lastname: Ritchie
authors.forename: X A
authors.initials: XA
authors.lastname: Conlan
authors.forename: R A
authors.initials: RA
authors.lastname: Shalliker
mesh_terms: D000601:Amino Acids, Essential / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation*; D057230:Limit of Detection; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D000601:Amino Acids, Essential
keywords: Active flow technology; Curtain flow; HPLC–MS/MS; Mass spectrometry