title: Trabecular titanium can induce in vitro osteogenic differentiation of human adipose derived stem cells without osteogenic factors.
abstract: Trabecular Titanium (TT) is an innovative highly porous structure that imitates the morphology of trabecular bone with good mechanical properties. Adipose-derived stem cells are a multipotent cell population that can be used in regenerative medicine, in particular, for bone therapeutic applications. The ability of TT to induce the osteogenic differentiation of human adipose derived stem cells (hASCs) in the absence of osteogenic factors was evaluated using molecular biological, biochemical, and immunohistochemical methods. At 7 and 21 days from differentiation, the hASCs grown on TT scaffolds showed similar expressions of alkaline phosphatase (ALP) and Runx-2 both in the presence and in the absence of osteogenic factors, as well as at transcript and protein levels. hASCs cultured on monolayer in the presence of the medium obtained from the wells where hASCs/scaffold constructs were cultured in the absence of osteogenic factors differentiated towards the osteogenic phenotype: their gene and protein expression of ALP and Runx-2 was similar to that of the same cells cultured in the presence of osteogenic factors, and significantly higher than that of the ones cultured in growth medium.
authors.affiliation: Department of Orthopedics and Traumatology, IRCCS Policlinico San Matteo Foundation, University of Pavia, Italy.
authors.forename: Francesco
authors.initials: F
authors.lastname: Benazzo
authors.forename: Laura
authors.initials: L
authors.lastname: Botta
authors.forename: Manuela Federica
authors.initials: MF
authors.lastname: Scaffino
authors.forename: Laura
authors.initials: L
authors.lastname: Caliogna
authors.forename: Matteo
authors.initials: M
authors.lastname: Marullo
authors.forename: Simonetta
authors.initials: S
authors.lastname: Fusi
authors.forename: Giulia
authors.initials: G
authors.lastname: Gastaldi
mesh_terms: D000273:Adipose Tissue / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D000469:Alkaline Phosphatase / Q000378:metabolism; D001483:Base Sequence; D002454:Cell Differentiation / Q000187:drug effects*; D050659:Core Binding Factor Alpha 1 Subunit / Q000378:metabolism; D017931:DNA Primers; D006801:Humans; D008855:Microscopy, Electron, Scanning; D010012:Osteogenesis; D060888:Real-Time Polymerase Chain Reaction; D013234:Stem Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D014025:Titanium / Q000494:pharmacology*
chemical_list: D050659:Core Binding Factor Alpha 1 Subunit; D017931:DNA Primers; C493709:RUNX2 protein, human; D014025:Titanium; D000469:Alkaline Phosphatase
keywords: Runx-2 expression; alkaline phosphatase expression; human adipose derived stem cell; osteoblast-like cell; osteogenic differentiation; trabecular titanium scaffold