title: Microsatellite instability affecting the T17 repeats in intron 8 of HSP110, as well as five mononucleotide repeats in patients with colorectal carcinoma.
abstract: OBJECTIVE
  To investigate mononucleotide markers: BAT-25, BAT-26, NR-21, NR-22 and NR-24 in patients with colorectal cancer (CRC), and the status of HSP110T17, KRAS, BRAF and the MLH1 promoter mutations in microsatellite unstable CRC.
  
  
  METHODS
  Genetic assessments were performed on samples obtained following resection of CRC in 200 patients.
  
  
  RESULTS
  Allelic variations of HSP110T17 were found in all 18 patients with microsatellite instabilities (MSIs) in at least three markers (high-frequency MSI). By contrast, mutations of HSP110T17 were absent in all 20 patients with no MSI frequency. Eight out of 182 patients with low (instability in one marker) or no frequency MSI had allelic shifts due to polymorphisms of BAT-25 (1.5%), NR-21 (1.75%) and NR-24 (1.5%). BRAF mutations were associated with >5 bp shortening of HSP110T17.
  
  
  CONCLUSIONS
  Patients with high-frequency MSI CRC had allelic variations of HSP110T17. BRAF mutations occur along with greater shortening in HSP110T17 during oncogenesis via the MSI pathway.
authors.affiliation: Center for Gastroenterology & Hepatology, University City Hospital, Belgrade, Serbia.
authors.forename: Srdjan
authors.initials: S
authors.lastname: Markovic
authors.forename: Jadranka
authors.initials: J
authors.lastname: Antic
authors.forename: Ivan
authors.initials: I
authors.lastname: Dimitrijevic
authors.forename: Branimir
authors.initials: B
authors.lastname: Zogovic
authors.forename: Daniela
authors.initials: D
authors.lastname: Bojic
authors.forename: Petar
authors.initials: P
authors.lastname: Svorcan
authors.forename: Velimir
authors.initials: V
authors.lastname: Markovic
authors.forename: Zoran
authors.initials: Z
authors.lastname: Krivokapic
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000473:pathology; D019175:DNA Methylation; D005260:Female; D050957:HSP110 Heat-Shock Proteins / Q000235:genetics*; D006801:Humans; D007438:Introns / Q000235:genetics*; D008297:Male; D053842:Microsatellite Instability; D018895:Microsatellite Repeats / Q000235:genetics*; D008875:Middle Aged; D000070957:MutL Protein Homolog 1; D009154:Mutation; D009687:Nuclear Proteins / Q000235:genetics; D010063:Ovum / Q000378:metabolism; D011110:Polymorphism, Genetic; D011518:Proto-Oncogene Proteins / Q000235:genetics; D048493:Proto-Oncogene Proteins B-raf / Q000235:genetics; D016283:Proto-Oncogene Proteins p21(ras); D013094:Spermatozoa / Q000378:metabolism; D018631:ras Proteins / Q000235:genetics
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D050957:HSP110 Heat-Shock Proteins; C117307:KRAS protein, human; C501486:MLH1 protein, human; D009687:Nuclear Proteins; D011518:Proto-Oncogene Proteins; C482119:BRAF protein, human; D048493:Proto-Oncogene Proteins B-raf; D000070957:MutL Protein Homolog 1; D016283:Proto-Oncogene Proteins p21(ras); D018631:ras Proteins