title: Multiplexed optical detection of plasma porphyrins using DNA aptamer-functionalized carbon nanotubes.
abstract: A novel optical platform based on DNA aptamer-functionalized SWCNTs (a-SWCNTs) is developed for multiplexed detection of plasma porphyrins. We have investigated the interactions of a-SWCNTs with heme (FePP), protoporphyrin (PP), coproporphyrin (CP), and uroporphyrin (UP). Two interaction mechanisms, specific binding, and nonspecific adsorption between porphyrins and a-SWCNTs are proposed based on observed optical signal modulations. The optical transduction signals are used to formulate a multiplexed detection strategy for the four porphyrin species without a laborious separation process. The detection scheme is sensitive, selective, and can readily be used for porphyrin detection in plasma samples when combined with a solvent extraction method. Our optical platform offers novel analytical tools for probing the surface chemistry at the porphyrin/a-SWCNTs interface, showing great promise for both research and clinical applications.
authors.affiliation: School of Mechanical Engineering, Birck Nanotechnology Center, Purdue University, West Lafayette, Indiana 47907, USA.
authors.forename: Jing
authors.initials: J
authors.lastname: Pan
authors.forename: Hanyu
authors.initials: H
authors.lastname: Zhang
authors.forename: Tae-Gon
authors.initials: TG
authors.lastname: Cha
authors.forename: Haorong
authors.initials: H
authors.lastname: Chen
authors.forename: Jong Hyun
authors.initials: JH
authors.lastname: Choi
mesh_terms: D000818:Animals; D052157:Aptamers, Nucleotide / Q000737:chemistry*; D002417:Cattle; D006801:Humans; D037742:Nanotubes, Carbon / Q000737:chemistry*; D011166:Porphyrins / Q000097:blood*; D019265:Spectroscopy, Near-Infrared / Q000379:methods*
chemical_list: D052157:Aptamers, Nucleotide; D037742:Nanotubes, Carbon; D011166:Porphyrins