title: Analysis of the cellular dynamics of fluorescently tagged glycosphingolipids by using a nanoliquid chromatography-tandem mass spectrometry platform.
abstract: Despite the increasing biological interests on glycoconjugates, the synthetic mechanism of oligosaccharides has not yet been revealed except for the enzymes involved. To clarify the synthetic events that occur inside cells, spatiotemporal analysis of fluorescently tagged glycosphingolipids was carried out. Transformation of the incorporated lactosylceramide analogue carrying 4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene-3-propionyl group (BODIPY fluorophore) was analyzed using nanoLC-fluorescence detection-nanoelectrospray ionization-mass spectrometry. A complex process of glycan synthesis and degradation was our primary observation.
authors.affiliation: Japan Science and Technology Agency (JST) , ERATO, 2-1 Hirosawa, Wako-shi, Saitama 351-0198 Japan.
authors.forename: Atsuko
authors.initials: A
authors.lastname: Ohtake
authors.forename: Shusaku
authors.initials: S
authors.lastname: Daikoku
authors.forename: Katsuhiko
authors.initials: K
authors.lastname: Suzuki
authors.forename: Yukishige
authors.initials: Y
authors.lastname: Ito
authors.forename: Osamu
authors.initials: O
authors.lastname: Kanie
mesh_terms: D000818:Animals; D019556:COS Cells; D002522:Chlorocebus aethiops; D002853:Chromatography, Liquid / Q000379:methods; D003593:Cytoplasm / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D006028:Glycosphingolipids / Q000032:analysis*; D036103:Nanotechnology / Q000379:methods*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D005456:Fluorescent Dyes; D006028:Glycosphingolipids