title: Using transposons for genetic mosaic analysis of plant development.
abstract: Genetic mosaics, or chimeras, are individual organisms composed of cells or tissues of two or more distinct genotypes. They are experimentally useful for addressing several key biological questions. These include fate mapping through analysis of marked clonal lineages, analyzing cell or tissue interactions such as the induction of developmental events, and analyzing whether a gene acts cell autonomously. Genetic mosaics can arise in many ways, including through the action of transposable elements. Naturally occurring transposons can generate genetic mosaics by somatically inserting into a gene to cause a mutant sector, somatically excising from a mutant gene to create a revertant wild-type sector, or causing chromosomal breaks or rearrangements leading to loss of a gene or genes. Transposons have also been cleverly engineered to allow the generation of marked somatic sectors, sometimes in controlled ways. Here we review ways in which transposon-induced genetic mosaics have been used experimentally, the various methods that have been used, and general considerations for designing genetic mosaic studies using transposon methods.
authors.affiliation: Department of Genetics, Development and Cell Biology, Iowa State University, Ames, IA, USA.
authors.forename: Philip W
authors.initials: PW
authors.lastname: Becraft
mesh_terms: D000483:Alleles; D002678:Chimera / Q000235:genetics*; D019457:Chromosome Breakage; D004251:DNA Transposable Elements / Q000235:genetics*; D020868:Gene Silencing; D005818:Genetic Engineering; D009154:Mutation / Q000235:genetics; D019895:Transposases / Q000378:metabolism; D003313:Zea mays / Q000235:genetics* / Q000254:growth & development*
chemical_list: D004251:DNA Transposable Elements; D019895:Transposases