title: Bacterial communities of the gorgonian octocoral Pseudopterogorgia elisabethae.
abstract: Pseudopterogorgia elisabethae is a common inhabitant of Caribbean reefs and is a well-known source of diterpenes with diverse biological activities. Notably, this octocoral is the sole source of the pseudopterosin family of anti-inflammatory diterpenes and is harvested to supply commercial demand for these metabolites. We have characterized the composition of the bacterial community associated with P. elisabethae collected from Providencia Island, Colombia, using both culture-dependent and culture-independent approaches. Culture-independent analysis revealed that the bacterial communities were composed of eight phyla, of which Proteobacteria was the most abundant. At the class level, bacterial communities were dominated by Gammaproteobacteria (82-87 %). Additionally, operational taxonomic units related to Pseudomonas and Endozoicomonas species were the most abundant phylotypes consistently associated with P. elisabethae colonies. Culture-dependent analysis resulted in the identification of 40 distinct bacteria classified as Bacilli (15), Actinobacteria (12), Gammaproteobacteria (9), Alphaproteobacteria (3), and Betaproteobacteria (1). Only one of the 40 cultured bacteria was closely related to a dominant phylotype detected in the culture-independent study, suggesting that conventional culturing techniques failed to culture the majority of octocoral-associated bacterial diversity. To the best of our knowledge, this is the first characterization of the bacterial diversity associated with P. elisabethae.
authors.affiliation: Departamento de Química, Universidad Nacional de Colombia, Bogotá, District of Colombia, Colombia.
authors.forename: Hebelin
authors.initials: H
authors.lastname: Correa
authors.forename: Brad
authors.initials: B
authors.lastname: Haltli
authors.forename: Carmenza
authors.initials: C
authors.lastname: Duque
authors.forename: Russell
authors.initials: R
authors.lastname: Kerr
mesh_terms: D000818:Animals; D037421:Anthozoa / Q000382:microbiology*; D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D044822:Biodiversity; D003105:Colombia; D008969:Molecular Sequence Data; D010802:Phylogeny
references.citation: Nat Prod Rep. 2012 Jul;29(7):739-51
references.pmid: 22660834
references.citation: J Mol Evol. 1980 Dec;16(2):111-20
references.pmid: 7463489
references.citation: FEMS Microbiol Ecol. 2007 Jan;59(1):81-94
references.pmid: 17233746
references.citation: FEMS Microbiol Lett. 2010 Aug 1;309(1):1-7
references.pmid: 20487025
references.citation: Nat Prod Rep. 2009 May;26(5):681-710
references.pmid: 19387501
references.citation: J Antibiot (Tokyo). 2010 Apr;63(4):191-3
references.pmid: 20150929
references.citation: Appl Environ Microbiol. 2008 Dec;74(23):7272-85
references.pmid: 18849454
references.citation: Appl Environ Microbiol. 2006 Feb;72(2):1680-3
references.pmid: 16461727
references.citation: Environ Microbiol. 2005 Aug;7(8):1162-74
references.pmid: 16011753
references.citation: J Antibiot (Tokyo). 2005 Jan;58(1):1-26
references.pmid: 15813176
references.citation: Bioinformatics. 2007 Nov 1;23(21):2947-8
references.pmid: 17846036
references.citation: Integr Comp Biol. 2010 Oct;50(4):662-74
references.pmid: 21558231
references.citation: Appl Environ Microbiol. 2009 Dec;75(23):7537-41
references.pmid: 19801464
references.citation: Microb Ecol. 2012 Jul;64(1):105-16
references.pmid: 22281804
references.citation: Int Microbiol. 1999 Dec;2(4):267-71
references.pmid: 10943423
references.citation: Nucleic Acids Res. 1989 Oct 11;17(19):7843-53
references.pmid: 2798131
references.citation: PLoS One. 2010 Apr 29;5(4):e10401
references.pmid: 20454460
references.citation: Appl Environ Microbiol. 2002 Aug;68(8):3878-85
references.pmid: 12147485
references.citation: Appl Environ Microbiol. 2009 Jun;75(11):3492-501
references.pmid: 19346350
references.citation: Curr Pharm Biotechnol. 2007 Oct;8(5):253-60
references.pmid: 17979723
references.citation: Trends Pharmacol Sci. 2010 Jun;31(6):255-65
references.pmid: 20363514
references.citation: PLoS One. 2012;7(1):e30580
references.pmid: 22276216
references.citation: Appl Environ Microbiol. 2007 Aug;73(16):5261-7
references.pmid: 17586664
references.citation: Mol Biol Evol. 1987 Jul;4(4):406-25
references.pmid: 3447015
references.citation: Mar Biotechnol (NY). 2007 Sep-Oct;9(5):561-76
references.pmid: 17514404
references.citation: FEMS Microbiol Ecol. 2009 May;68(2):152-63
references.pmid: 19302548
references.citation: FEMS Microbiol Ecol. 2011 Apr;76(1):109-20
references.pmid: 21223327
references.citation: Microb Ecol. 2012 Apr;63(3):605-18
references.pmid: 21984347
references.citation: Science. 1997 May 2;276(5313):734-40
references.pmid: 9115194
references.citation: ISME J. 2007 Nov;1(7):654-9
references.pmid: 18043672
references.citation: Poult Sci. 2009 Feb;88(2):298-302
references.pmid: 19151343
references.citation: Environ Microbiol. 2011 May;13(5):1192-204
references.pmid: 21272183
references.citation: Mar Drugs. 2010 Apr 22;8(4):1417-68
references.pmid: 20479984
references.citation: Mar Drugs. 2011 Mar 04;9(3):334-43
references.pmid: 21556163
references.citation: Nat Prod Rep. 2008 Feb;25(1):35-94
references.pmid: 18250897
references.citation: PLoS One. 2010 Mar 05;5(3):e9554
references.pmid: 20221265
references.citation: J Ind Microbiol Biotechnol. 2010 Mar;37(3):219-24
references.pmid: 20033830
references.citation: Mar Drugs. 2012 Jul;10(7):1566-71
references.pmid: 22851926
references.citation: PLoS One. 2011;6(12):e27310
references.pmid: 22194782
references.citation: Appl Environ Microbiol. 2011 Feb;77(3):862-70
references.pmid: 21115701
references.citation: Appl Environ Microbiol. 2007 May;73(10):3272-82
references.pmid: 17400789
references.citation: Proc Natl Acad Sci U S A. 1992 Apr 15;89(8):3639-43
references.pmid: 1565660
references.citation: ISME J. 2013 Jul;7(7):1452-8
references.pmid: 23303372
references.citation: Chem Biol. 2003 Nov;10(11):1051-6
references.pmid: 14652072
references.citation: Environ Microbiol. 2010 Jan;12(1):28-39
references.pmid: 19691500
references.citation: Environ Microbiol. 2007 May;9(5):1291-305
references.pmid: 17472641
references.citation: ISME J. 2008 Apr;2(4):350-63
references.pmid: 18059490
references.citation: Int J Syst Bacteriol. 1997 Apr;47(2):590-2
references.pmid: 9103655
references.citation: Mol Biol Evol. 2007 Aug;24(8):1596-9
references.pmid: 17488738
references.citation: Nature. 1990 May 3;345(6270):63-5
references.pmid: 1691827
references.citation: World J Microbiol Biotechnol. 2013 Jun;29(6):1107-16
references.pmid: 23386321
references.citation: Nat Chem Biol. 2006 Dec;2(12):666-73
references.pmid: 17108984
references.citation: Evolution. 1985 Jul;39(4):783-791
references.pmid: 28561359
references.citation: Nucleic Acids Res. 2009 Jan;37(Database issue):D141-5
references.pmid: 19004872
references.citation: Environ Microbiol. 2006 Dec;8(12):2068-73
references.pmid: 17107548
references.citation: J Mol Biol. 1990 Oct 5;215(3):403-10
references.pmid: 2231712
references.citation: J Pharm Bioallied Sci. 2012 Jan;4(1):66-9
references.pmid: 22368402