title: A new role for glutathione in the regulation of root architecture linked to strigolactones.
abstract: Reduced glutathione (GSH) is required for root development, but its functions are not characterized. The effects of GSH depletion on root development were therefore studied in relation to auxin and strigolactone (SL) signalling using a combination of molecular genetic approaches and pharmacological techniques. Lateral root (LR) density was significantly decreased in GSH synthesis mutants (cad2-1, pad2-, rax1-), but not by the GSH synthesis inhibitor, buthionine sulfoximine (BSO). BSO-induced GSH depletion therefore did not influence root architecture in the same way as genetic impairment. Root glutathione contents were similar in the wild-type seedlings and max3-9 and max4-1 mutants that are deficient in SL synthesis and in the SL-signalling mutant, max2-1. BSO-dependent inhibition of GSH synthesis depleted the tissue GSH pool to a similar extent in the wild-type and SL synthesis mutants, with no effect on LR density. The application of the SL analogue GR24 increased root glutathione in the wild-type, max3-9 and max4-1 seedlings, but this increase was absent from max2-1. Taken together, these data establish a link between SLs and the GSH pool that occurs in a MAX2-dependent manner.
authors.affiliation: Centre for Plant Sciences, Faculty of Biology, University of Leeds, Leeds, LS2 9JT, UK; Department of Plant Systems Biology, VIB, Ghent University, 9052, Gent, Belgium; Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052, Gent, Belgium.
authors.forename: Belen
authors.initials: B
authors.lastname: Marquez-Garcia
authors.forename: Maria
authors.initials: M
authors.lastname: Njo
authors.forename: Tom
authors.initials: T
authors.lastname: Beeckman
authors.forename: Sofie
authors.initials: S
authors.lastname: Goormachtig
authors.forename: Christine H
authors.initials: CH
authors.lastname: Foyer
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D005978:Glutathione / Q000378:metabolism / Q000502:physiology*; D005981:Glutathione Synthase / Q000235:genetics; D007210:Indoleacetic Acids / Q000378:metabolism / Q000494:pharmacology; D007783:Lactones / Q000378:metabolism*; D010937:Plant Growth Regulators / Q000378:metabolism; D018517:Plant Roots / Q000033:anatomy & histology / Q000254:growth & development / Q000378:metabolism
chemical_list: D007210:Indoleacetic Acids; D007783:Lactones; D010937:Plant Growth Regulators; D005981:Glutathione Synthase; D005978:Glutathione
keywords: auxin; hormone signalling; lateral roots; meristem; redox signaling