title: Mesoporous silicon particles for sustained gene silencing.
abstract: RNA interference (RNAi) is a powerful approach for silencing oncogenes; however, in vivo RNAi delivery has remained a major challenge due to lack of safe, efficient, and sustained delivery. Here, we describe a novel approach to overcome these limitations using mesoporous silicon particles loaded with nanoparticles (i.e., liposomes) containing small interfering RNA (siRNA) targeted against oncoprotein that contributes to cancer cell survival. This delivery method resulted in sustained gene silencing for at least 3 weeks with substantial reduction of tumor growth with no overt toxicities in two independent orthotopic mouse models of ovarian cancer following a single intravenous administration of mesoporous silicon particles loaded with liposomal EphA2-siRNA.
authors.affiliation: Thomas Jefferson University, Philadelphia, PA, USA.
authors.forename: Nafis
authors.initials: N
authors.lastname: Hasan
authors.forename: Aman
authors.initials: A
authors.lastname: Mann
authors.forename: Mauro
authors.initials: M
authors.lastname: Ferrari
authors.forename: Takemi
authors.initials: T
authors.lastname: Tanaka
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D005260:Female; D020868:Gene Silencing; D018014:Gene Transfer Techniques; D015316:Genetic Therapy / Q000379:methods; D006801:Humans; D008081:Liposomes / Q000008:administration & dosage; D051379:Mice; D053758:Nanoparticles / Q000008:administration & dosage; D010051:Ovarian Neoplasms / Q000235:genetics* / Q000473:pathology / Q000628:therapy; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000008:administration & dosage*; D012825:Silicon / Q000008:administration & dosage
chemical_list: D008081:Liposomes; D034741:RNA, Small Interfering; D012825:Silicon