title: Algivirga pacifica gen. nov., sp. nov., a novel agar-degrading marine bacterium of the family Flammeovirgaceae isolated from Micronesia.
abstract: An aerobic, Gram-negative, coccoid to short rod-shaped and non-flagellated marine bacterial strain S354(T) was isolated from seawater of Micronesia. The strain was capable to degrade agar-forming slight depression into agar plate. Growth occurred at a temperature range of 12-44 °C, a pH range of 5-9, and a salinity range of 1-7 % (w/v) NaCl. Phylogenetic analyses based on 16S rRNA gene sequences suggested that S354(T) belongs to the family Flammeovirgaceae. The novel strain was most closely related to Limibacter armeniacum YM 11-185(T) with similarity of 92.5 %. The DNA G+C content was 43.8 mol%. The major fatty acids (>10 %) were iso-C15:0 and C16:1 ω5c. The predominant isoprenoid quinone was determined to be MK-7. Polar lipid profile of S354(T) consisted of phosphatidylethanolamine, unknown polar lipid, and unknown glycolipids. Based on the phenotypic, phylogenetic, biochemical, and physiological tests conducted in this study, S354(T) is proposed to represent a type strain of a novel genus and species. The 16S rRNA gene sequence of S354(T) is registered in GenBank under the accession number JQ639084. The type of strain Algivirga pacifica gen. nov., sp. nov. is S354(T) (=KCCM 90107(T)=JCM 18326(T)).
authors.affiliation: Korea Institute of Ocean Science and Technology, Ansan, 426-744, Republic of Korea.
authors.forename: Jennifer Jooyoun
authors.initials: JJ
authors.lastname: Kim
authors.forename: Ji Hyung
authors.initials: JH
authors.lastname: Kim
authors.forename: Young-Kyung
authors.initials: YK
authors.lastname: Kwon
authors.forename: Kae Kyoung
authors.initials: KK
authors.lastname: Kwon
authors.forename: Sung-Hyun
authors.initials: SH
authors.lastname: Yang
authors.forename: Jiyi
authors.initials: J
authors.lastname: Jang
authors.forename: Soo-Jin
authors.initials: SJ
authors.lastname: Heo
authors.forename: Heung-Sik
authors.initials: HS
authors.lastname: Park
authors.forename: Won-Kyo
authors.initials: WK
authors.lastname: Jung
authors.forename: Youngdeuk
authors.initials: Y
authors.lastname: Lee
authors.forename: Do-Hyung
authors.initials: DH
authors.lastname: Kang
authors.forename: Chulhong
authors.initials: C
authors.lastname: Oh
mesh_terms: D000362:Agar / Q000378:metabolism*; D015373:Bacterial Typing Techniques; D041963:Bacteroidetes / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D001482:Base Composition; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D008849:Micronesia; D008855:Microscopy, Electron, Scanning; D008969:Molecular Sequence Data; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D011809:Quinones / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride / Q000378:metabolism; D013696:Temperature
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D010743:Phospholipids; D011809:Quinones; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride; D000362:Agar
references.citation: Appl Environ Microbiol. 1997 Aug;63(8):3068-78
references.pmid: 9251193
references.citation: J Mol Evol. 1980 Dec;16(2):111-20
references.pmid: 7463489
references.citation: Antonie Van Leeuwenhoek. 2011 Jun;100(1):145-53
references.pmid: 21416335
references.citation: Int J Syst Evol Microbiol. 2010 Aug;60(Pt 8):1858-1863
references.pmid: 19767357
references.citation: Mol Biol Evol. 1987 Jul;4(4):406-25
references.pmid: 3447015
references.citation: Mol Biol Evol. 2011 Oct;28(10):2731-9
references.pmid: 21546353
references.citation: Int J Syst Evol Microbiol. 2007 May;57(Pt 5):1046-1049
references.pmid: 17473256
references.citation: Evolution. 1985 Jul;39(4):783-791
references.pmid: 28561359
references.citation: Int J Syst Evol Microbiol. 2005 Jan;55(Pt 1):231-234
references.pmid: 15653879
references.citation: Int J Syst Evol Microbiol. 2013 Mar;63(Pt 3):1105-1110
references.pmid: 22707538
references.citation: Antonie Van Leeuwenhoek. 2012 Jan;101(1):133-40
references.pmid: 21789597
references.citation: Int J Syst Evol Microbiol. 2011 Oct;61(Pt 10):2342-2347
references.pmid: 21037036
references.citation: Int J Syst Evol Microbiol. 2007 Oct;57(Pt 10):2275-2279
references.pmid: 17911296
references.citation: J Mol Evol. 1981;17(6):368-76
references.pmid: 7288891
references.citation: Int J Syst Evol Microbiol. 2000 Sep;50 Pt 5:1861-1868
references.pmid: 11034497
references.citation: Int J Syst Evol Microbiol. 2011 Jul;61(Pt 7):1521-1527
references.pmid: 20656812
references.citation: Int J Syst Evol Microbiol. 2008 Apr;58(Pt 4):982-6
references.pmid: 18398206
references.citation: Int J Syst Evol Microbiol. 2013 May;63(Pt 5):1639-1645
references.pmid: 22904215
references.citation: Int J Syst Evol Microbiol. 2006 Sep;56(Pt 9):2095-2100
references.pmid: 16957105
references.citation: Appl Environ Microbiol. 2000 Dec;66(12):5116-22
references.pmid: 11097877
references.citation: Int J Syst Evol Microbiol. 2007 Aug;57(Pt 8):1689-1693
references.pmid: 17684238
references.citation: Int J Syst Evol Microbiol. 2007 May;57(Pt 5):964-968
references.pmid: 17473242
references.citation: Int J Syst Evol Microbiol. 2006 May;56(Pt 5):1059-1065
references.pmid: 16627655
references.citation: Appl Environ Microbiol. 2002 Jan;68(1):201-10
references.pmid: 11772628
references.citation: Can J Microbiol. 1970 Jun;16(6):511-5
references.pmid: 5423286
references.citation: Int J Syst Evol Microbiol. 2005 Nov;55(Pt 6):2583-2588
references.pmid: 16280531