title: Effect of chromatographic conditions on enantioseparation of bovine serum albumin chiral stationary phase in HPLC and thermodynamic studies.
abstract: Twelve chiral compounds were enantiomerically resolved on bovine serum albumin chiral stationary phase (BSA-CSP) by high-performance liquid chromatography (HPLC) in reversed-phase modes. Chromatographic conditions such as mobile phase pH, the percentage of organic modifier, and concentration of analyte were optimized for separation of enantiomers. For N-(2, 4-dinitrophenyl)-serine (DNP-ser), the retention factors (k) greatly increase from 0.81 to 6.23 as the pH decreasing from 7.21 to 5.14, and the resolution factor (R(s)) exhibited a similar increasing trend (from 0 to 1.34). More interestingly, the retention factors for N-(2, 4-dinitrophenyl)-proline (DNP-pro) decrease along with increasing 1-propanol in mobile phase (3%, 5%, 7% and 9% by volume), whereas the resolution factor shows an upward trend (from 0.96 to 2.04). Moreover, chiral recognition mechanisms for chiral analytes were further investigated through thermodynamic methods.
authors.affiliation: Institute of Special Materials/School of Chemistry and Environment, South China Normal University, Guangzhou, China.
authors.forename: Qiu-Yun
authors.initials: QY
authors.lastname: Wang
authors.forename: Ya-Jin
authors.initials: YJ
authors.lastname: Xiong
authors.forename: Bao-Zhu
authors.initials: BZ
authors.lastname: Lu
authors.forename: Jun
authors.initials: J
authors.lastname: Fan
authors.forename: Sheng-Run
authors.initials: SR
authors.lastname: Zheng
authors.forename: Wei-Guang
authors.initials: WG
authors.lastname: Zhang
mesh_terms: D000818:Animals; D001573:Benzoin / Q000032:analysis / Q000737:chemistry; D002417:Cattle; D002623:Chemistry Techniques, Analytical / Q000295:instrumentation / Q000592:standards; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000592:standards*; D008333:Mandelic Acids / Q000032:analysis / Q000737:chemistry; D015394:Molecular Structure; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D013237:Stereoisomerism; D013816:Thermodynamics
chemical_list: D008333:Mandelic Acids; D012710:Serum Albumin, Bovine; D001573:Benzoin; C037938:mandelic acid
keywords: HPLC; amino acid derivatives; bovine serum albumin chiral stationary phase; chiral separation; thermodynamics