title: Structure of dihydrouridine synthase C (DusC) from Escherichia coli.
abstract: Dihydrouridine (D) is one of the most widely conserved tRNA modifications. Dihydrouridine synthase (Dus) is responsible for introducing D modifications into RNA by the reduction of uridine. Recently, a unique substrate-recognition mechanism using a small adapter molecule has been proposed for Thermus thermophilus Dus (TthDusC). To acquire insight regarding its substrate-recognition mechanism, the crystal structure of DusC from Escherichia coli (EcoDusC) was determined at 2.1 Å resolution. EcoDusC was shown to be composed of two domains: an N-terminal catalytic domain and a C-terminal tRNA-binding domain. An L-shaped electron density surrounded by highly conserved residues was found in the active site, as observed for TthDus. Structure comparison with TthDus indicated that the N-terminal region has a similar structure, whereas the C-terminal domain has marked differences in its relative orientation to the N-terminal domain as well as in its own structure. These observations suggested that Dus proteins adopt a common substrate-recognition mechanism using an adapter molecule, whereas the manner of tRNA binding is diverse.
authors.affiliation: Graduate School of Life Sciences, Hokkaido University, Sapporo 060-0810, Japan.
authors.forename: Minghao
authors.initials: M
authors.lastname: Chen
authors.forename: Jian
authors.initials: J
authors.lastname: Yu
authors.forename: Yoshikazu
authors.initials: Y
authors.lastname: Tanaka
authors.forename: Miyuki
authors.initials: M
authors.lastname: Tanaka
authors.forename: Isao
authors.initials: I
authors.lastname: Tanaka
authors.forename: Min
authors.initials: M
authors.lastname: Yao
mesh_terms: D020134:Catalytic Domain / Q000235:genetics; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics; D029968:Escherichia coli Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D010088:Oxidoreductases / Q000737:chemistry* / Q000378:metabolism; D017433:Protein Structure, Secondary; D012329:RNA, Bacterial / Q000737:chemistry; D012343:RNA, Transfer / Q000737:chemistry; D013379:Substrate Specificity / Q000235:genetics; D014961:X-Ray Diffraction
chemical_list: D029968:Escherichia coli Proteins; D012329:RNA, Bacterial; D012343:RNA, Transfer; D010088:Oxidoreductases
keywords: dihydrouridine; dihydrouridine synthase; tRNA modification
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