title: Bacterial bioeffectors modify bioactive profile and increase isoflavone content in soybean sprouts (Glycine max var Osumi).
abstract: The effect of two bacterial strains to enhance bioactive contents (total phenolic compounds, total flavonoid compounds and isoflavones) and antioxidant activity on 3-day-old soybean sprouts were investigated. To identify bacterial determinants responsible for these effects, viable and UV-treated strains were delivered to wounded seeds at different concentration. Multivariate analysis performed with all the evaluated parameters indicated the different effectiveness of Stenotrophomonas maltophilia N5.18 and Pseudomonas fluorescens N21.4 based on different structural and metabolic determinants for each. N21.4 increased total phenolics and isoflavones from the genistein family, while N5.18 triggered biosynthesis of daidzein and genistein families coupled to a decrease in total phenolics, suggesting different molecular targets in the phenilpropanoid pathway. Only extracts from N5.18 treated seeds showed an improved antioxidant activity according to the β-carotene bleaching prevention method. In summary, bioeffectors from both bacterial strains are effective tools to improve soybean sprouts quality; structural elicitors from N5.18 also enhanced antioxidant activity, being the best alternative for further development of a biotechnological procedure.
authors.affiliation: Universidad CEU San Pablo, Facultad de Farmacia, Boadilla del Monte, Madrid, Spain. elalgar@ceu.es
authors.forename: Elena
authors.initials: E
authors.lastname: Algar
authors.forename: Beatriz
authors.initials: B
authors.lastname: Ramos-Solano
authors.forename: Ana
authors.initials: A
authors.lastname: García-Villaraco
authors.forename: M Dolores Saco
authors.initials: MD
authors.lastname: Sierra
authors.forename: M Soledad Martín
authors.initials: MS
authors.lastname: Gómez
authors.forename: F Javier
authors.initials: FJ
authors.lastname: Gutiérrez-Mañero
mesh_terms: D000975:Antioxidants / Q000032:analysis; D005419:Flavonoids / Q000032:analysis; D007529:Isoflavones / Q000032:analysis*; D015999:Multivariate Analysis; D010636:Phenols / Q000032:analysis; D018520:Plant Shoots / Q000737:chemistry / Q000378:metabolism*; D011551:Pseudomonas fluorescens / Q000502:physiology* / Q000528:radiation effects; D012639:Seeds / Q000737:chemistry / Q000382:microbiology / Q000502:physiology; D013025:Glycine max / Q000737:chemistry / Q000254:growth & development / Q000378:metabolism*; D020615:Stenotrophomonas maltophilia / Q000502:physiology* / Q000528:radiation effects; D014466:Ultraviolet Rays
chemical_list: D000975:Antioxidants; D005419:Flavonoids; D007529:Isoflavones; D010636:Phenols
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