title: Pulmonary immune responses to Aspergillus fumigatus in an immunocompetent mouse model of repeated exposures.
abstract: Aspergillus fumigatus is a filamentous fungus that produces abundant pigmented conidia. Several fungal components have been identified as virulence factors, including melanin; however, the impact of these factors in a repeated exposure model resembling natural environmental exposures remains unknown. This study examined the role of fungal melanin in the stimulation of pulmonary immune responses using immunocompetent BALB/c mice in a multiple exposure model. It compared conidia from wild-type A. fumigatus to two melanin mutants of the same strain, Δarp2 (tan) or Δalb1 (white). Mass spectrometry-based analysis of conidial extracts demonstrated that there was little difference in the protein fingerprint profiles between the three strains. Field emission scanning electron microscopy demonstrated that the immunologically inert Rodlet A layer remained intact in melanin-deficient conidia. Thus, the primary difference between the strains was the extent of melanization. Histopathology indicated that each A. fumigatus strain induced lung inflammation, regardless of the extent of melanization. In mice exposed to Δalb1 conidia, an increase in airway eosinophils and a decrease in neutrophils and CD8(+) IL-17(+) (Tc17) cells were observed. Additionally, it was shown that melanin mutant conidia were more rapidly cleared from the lungs than wild-type conidia. These data suggest that the presence of fungal melanin may modulate the pulmonary immune response in a mouse model of repeated exposures to A. fumigatus conidia.
authors.affiliation: Allergy and Clinical Immunology Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Centers for Disease Control and Prevention , Morgantown, WV , USA .
authors.forename: Amanda D
authors.initials: AD
authors.lastname: Buskirk
authors.forename: Steven P
authors.initials: SP
authors.lastname: Templeton
authors.forename: Ajay P
authors.initials: AP
authors.lastname: Nayak
authors.forename: Justin M
authors.initials: JM
authors.lastname: Hettick
authors.forename: Brandon F
authors.initials: BF
authors.lastname: Law
authors.forename: Brett J
authors.initials: BJ
authors.lastname: Green
authors.forename: Donald H
authors.initials: DH
authors.lastname: Beezhold
mesh_terms: D000818:Animals; D001232:Aspergillus fumigatus / Q000276:immunology*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D004781:Environmental Exposure; D005260:Female; D008168:Lung / Q000276:immunology*; D008543:Melanins / Q000502:physiology; D051379:Mice; D008807:Mice, Inbred BALB C; D023421:Models, Animal; D009504:Neutrophils / Q000276:immunology; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D008543:Melanins
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