title: Transcriptome profiling of the whitefly Bemisia tabaci reveals stage-specific gene expression signatures for thiamethoxam resistance.
abstract: Bemisia tabaci has developed high levels of resistance to many insecticides including the neonicotinoids and there is strong evidence that for some compounds resistance is stage-specific. To investigate the molecular basis of B. tabaci resistance to the neonicotinoid thiamethoxam we used a custom whitefly microarray to compare gene expression in the egg, nymph and adult stages of a thiamethoxam-resistant strain (TH-R) with a susceptible strain (TH-S). Gene ontology and bioinformatic analyses revealed that in all life stages many of the differentially expressed transcripts encoded enzymes involved in metabolic processes and/or metabolism of xenobiotics. Several of these are candidate resistance genes and include the cytochrome P450 CYP6CM1, which has been shown to confer resistance to several neonicotinoids previously, a P450 belonging to the Cytochrome P450s 4 family and a glutathione S-transferase (GST) belonging to the sigma class. Finally several ATP-binding cassette transporters of the ABCG subfamily were highly over-expressed in the adult stage of the TH-R strain and may play a role in resistance by active efflux. Here, we evaluated both common and stage-specific gene expression signatures and identified several candidate resistance genes that may underlie B. tabaci resistance to thiamethoxam.
authors.affiliation: Department of Plant Protection, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: N
authors.initials: N
authors.lastname: Yang
authors.forename: W
authors.initials: W
authors.lastname: Xie
authors.forename: C M
authors.initials: CM
authors.lastname: Jones
authors.forename: C
authors.initials: C
authors.lastname: Bass
authors.forename: X
authors.initials: X
authors.lastname: Jiao
authors.forename: X
authors.initials: X
authors.lastname: Yang
authors.forename: B
authors.initials: B
authors.lastname: Liu
authors.forename: R
authors.initials: R
authors.lastname: Li
authors.forename: Y
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000818:Animals; D005260:Female; D020869:Gene Expression Profiling; D006430:Hemiptera / Q000235:genetics*; D007305:Insecticide Resistance / Q000187:drug effects / Q000235:genetics*; D008018:Life Cycle Stages / Q000187:drug effects; D000073943:Neonicotinoids; D009574:Nitro Compounds; D020411:Oligonucleotide Array Sequence Analysis; D010078:Oxazines; D020133:Reverse Transcriptase Polymerase Chain Reaction; D000077922:Thiamethoxam; D013844:Thiazoles; D059467:Transcriptome / Q000187:drug effects / Q000235:genetics*
chemical_list: D000073943:Neonicotinoids; D009574:Nitro Compounds; D010078:Oxazines; D013844:Thiazoles; D000077922:Thiamethoxam
keywords: Bemisia tabaci; metabolic resistance; microarray; stage specific; thiamethoxam
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