title: Interaction between amylose and 1-butanol during 1-butanol-hydrochloric acid hydrolysis of normal rice starch.
abstract: The aim of this study was to examine the interaction between amylose and 1-butanol during the 1-butanol-hydrochloric acid (1-butanol-HCl) hydrolysis of normal rice starch. The interaction model between amylose and 1-butanol was proposed using gas chromatography-mass spectrometry (GC-MS), (13)C cross polarization and magic angle spinning NMR analysis ((13)C CP/MAS NMR), differential scanning calorimetry (DSC), and thermalgravimetric analysis (TGA). GC-MS data showed that another form of 1-butanol existed in 1-butanol-HCl-hydrolyzed normal rice starch, except in the form of free molecules absorbed on the starch granules. The signal of 1-butanol-HCl-hydrolyzed starch at 100.1 ppm appeared in the (13)C CP/MAS NMR spectrum, indicating that the amylose-1-butanol complex was formed. DSC and TGA data also demonstrated the formation of the complex, which significantly affected the thermal properties of normal rice starch. These findings revealed that less dextrin with low molecular weight formed might be attributed to resistance of this complex to acid during 1-butanol-HCl hydrolysis.
authors.affiliation: The State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China; School of Food Science and Technology, Jiangnan University, Wuxi 214122, China.
authors.forename: Xiuting
authors.initials: X
authors.lastname: Hu
authors.forename: Benxi
authors.initials: B
authors.lastname: Wei
authors.forename: Bao
authors.initials: B
authors.lastname: Zhang
authors.forename: Hongyan
authors.initials: H
authors.lastname: Li
authors.forename: Xueming
authors.initials: X
authors.lastname: Xu
authors.forename: Zhengyu
authors.initials: Z
authors.lastname: Jin
authors.forename: Yaoqi
authors.initials: Y
authors.lastname: Tian
mesh_terms: D020001:1-Butanol / Q000737:chemistry*; D000688:Amylose / Q000737:chemistry*; D002152:Calorimetry, Differential Scanning; D008401:Gas Chromatography-Mass Spectrometry; D006851:Hydrochloric Acid / Q000737:chemistry*; D006868:Hydrolysis; D019906:Nuclear Magnetic Resonance, Biomolecular; D012275:Oryza / Q000737:chemistry*; D013213:Starch / Q000737:chemistry*; D013816:Thermodynamics
chemical_list: D020001:1-Butanol; D013213:Starch; D000688:Amylose; D006851:Hydrochloric Acid
keywords: 1-butanol-HCl hydrolysis; Amylose-1-butanol complex; Normal rice starch