title: Near-IR-triggered photothermal/photodynamic dual-modality therapy system via chitosan hybrid nanospheres.
abstract: Gold nanorods (AuNR)- and indocyanine green (ICG)-encapsulated chitosan hybrid nanospheres (CS-AuNR-ICG NSs) were successfully prepared and used for photothermal and photodynamic combined therapy with a single irradiation. These nanospheres were characterized by transmission electron microscopy, dynamic light scattering and UV-Vis absorption spectra. The in vivo anticancer effects of the hybrid nanospheres were examined by photodynamic therapy (PDT), photothermal therapy (PTT), and PTT/PDT combined therapy. It was found that the hybrid nanospheres had spherical size of 180 nm and a broad adsorption from 650 nm to 900 nm. The spherical chitosan matrix could effectively load ICG and protect it from the rapid hydrolysis. In vivo near-infrared fluorescence imaging and biodistribution demonstrated that ICG and AuNR could be selectively delivered to the tumor site with high accumulation. With the irradiation by 808 nm laser, chitosan hybrid nanospheres were capable to simultaneously produce sufficient hyperthermia and reactive oxygen species to kill cancer cells at irradiation sites, resulting in the complete tumor disappearance in the most of tumor-bearing mice. Compared with photothermal therapy or photodynamic therapy alone, the combined therapy had a significantly synergistic effect and improved the therapeutic efficacy.
authors.affiliation: Laboratory of Mesoscopic Chemistry and Department of Polymer Science & Engineering, College of Chemistry & Chemical Engineering, Nanjing University, Nanjing 210093, PR China.
authors.forename: Rui
authors.initials: R
authors.lastname: Chen
authors.forename: Xin
authors.initials: X
authors.lastname: Wang
authors.forename: Xikuan
authors.initials: X
authors.lastname: Yao
authors.forename: Xianchuang
authors.initials: X
authors.lastname: Zheng
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.forename: Xiqun
authors.initials: X
authors.lastname: Jiang
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D048271:Chitosan / Q000737:chemistry*; D006046:Gold / Q000737:chemistry; D006367:HeLa Cells; D006801:Humans; D007208:Indocyanine Green / Q000737:chemistry; D051379:Mice; D054118:Nanospheres / Q000737:chemistry*; D043942:Nanotubes / Q000737:chemistry; D010778:Photochemotherapy / Q000379:methods*; D017382:Reactive Oxygen Species / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D017382:Reactive Oxygen Species; D014409:Tumor Necrosis Factor-alpha; D006046:Gold; D048271:Chitosan; D007208:Indocyanine Green
keywords: Chitosan; Gold nanorods; Indocyanine green; Photodynamic therapy; Photothermal therapy