title: Expression, purification, crystallization and preliminary crystallographic analysis of spermidine acetyltransferase from Escherichia coli.
abstract: The spermidine acetyltransferase (SAT) from Escherichia coli catalyses the transfer of acetyl groups from acetyl-CoA to spermidine. SAT has been expressed and purified from E. coli. SAT was crystallized by the sitting-drop vapour-diffusion method to obtain a more detailed insight into the molecular mechanism. Preliminary X-ray diffraction studies revealed that the crystals diffracted to 2.5 Å resolution and belonged to the cubic space group P23, with unit-cell parameters a = b = c = 148.7 Å. They contained four molecules per asymmetric unit.
authors.affiliation: Graduate School of Science, Osaka University, Suita, Osaka 565-0871, Japan.
authors.forename: Mayumi
authors.initials: M
authors.lastname: Niiyama
authors.forename: Shigeru
authors.initials: S
authors.lastname: Sugiyama
authors.forename: Mika
authors.initials: M
authors.lastname: Hirose
authors.forename: Sae
authors.initials: S
authors.lastname: Ishikawa
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Tomitori
authors.forename: Kyohei
authors.initials: K
authors.lastname: Higashi
authors.forename: Tomoko
authors.initials: T
authors.lastname: Yamashita
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Adachi
authors.forename: Kazufumi
authors.initials: K
authors.lastname: Takano
authors.forename: Satoshi
authors.initials: S
authors.lastname: Murakami
authors.forename: Michio
authors.initials: M
authors.lastname: Murata
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Inoue
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Mori
authors.forename: Keiko
authors.initials: K
authors.lastname: Kashiwagi
authors.forename: Hiroyoshi
authors.initials: H
authors.lastname: Matsumura
authors.forename: Kazuei
authors.initials: K
authors.lastname: Igarashi
mesh_terms: D000123:Acetyltransferases / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification*; D003460:Crystallization; D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000201:enzymology*; D029968:Escherichia coli Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification*; D015964:Gene Expression Regulation, Bacterial; D000099228:Diamine N-Acetyltransferase
chemical_list: D000123:Acetyltransferases; D029968:Escherichia coli Proteins; D000099228:Diamine N-Acetyltransferase
keywords: Escherichia coli; spermidine acetyltransferase
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