title: Synthesis, Stability, Cellular Uptake, and Blood Circulation Time of Carboxymethyl-Inulin Coated Magnetic Nanoparticles.
abstract: Iron oxide nanoparticles were coated with the biocompatible, biodegradable, non-immunogenic polysaccharide inulin by introduction of carboxyl groups into the inulin structure and conjugation with amine groups on the surface of iron oxide nanoparticles grafted with 3-aminopropyltriethoxysilane. The resulting nanoparticles were characterized by FT-IR spectroscopy, transmission electron microscopy, dynamic light scattering, zeta potential, SQUID magnetometry, and with respect to their energy dissipation rate in applied alternating magnetic fields. The nanoparticles had a hydrodynamic diameter in the range of 70 ± 10 nm and were superparamagnetic, with energy dissipation rates in the range of 58-175 W/g for an applied field frequency of 233 kHz and an applied field amplitude in the range of 20-48 kA/m. The nanoparticles were stable in a range of pH, at temperatures between 23°C and 53°C, and in short term storage in water, PBS, and culture media. The particles were non-cytotoxic to the immortalized human cancer cell lines Hey A8 FDR, A2780, MDA 468, MCF-7 and Caco-2. The nanoparticles were readily taken up by Caco-2 cells in a time and concentration dependent fashion, and were found to have a pharmacokinetic time constant of 47 ± 3 min. The small size, non-cytotoxicity, and efficient energy dissipation of the particles could make them useful for biomedical applications such as magnetic fluid hyperthermia.
authors.affiliation: Department of Chemical Engineering, University of Puerto Rico, Mayagüez Campus, P.O. Box 9000, Mayagüez PR 00681, Puerto Rico.
authors.forename: Lenibel
authors.initials: L
authors.lastname: Santiago-Rodríguez
authors.forename: Moises Montalvo
authors.initials: MM
authors.lastname: Lafontaine
authors.forename: Cristina
authors.initials: C
authors.lastname: Castro
authors.forename: Janet
authors.initials: J
authors.lastname: Méndez-Vega
authors.forename: Magda
authors.initials: M
authors.lastname: Latorre-Esteves
authors.forename: Eduardo J
authors.initials: EJ
authors.lastname: Juan
authors.forename: Edna
authors.initials: E
authors.lastname: Mora
authors.forename: Madeline
authors.initials: M
authors.lastname: Torres-Lugo
authors.forename: Carlos
authors.initials: C
authors.lastname: Rinaldi
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