title: Cbf14 copy number variation in the A, B, and D genomes of diploid and polyploid wheat.
abstract: Freezing tolerance and winter hardiness are complex traits. In the Triticeae, two loci on the group 5 chromosome homoeologs are repeatedly identified as having major effects on these traits. Recently, we found that segments of the genomic region at one of these loci, Frost resistance-2 (Fr-2) is copy number variable in barley. Freezing-tolerant winter-hardy genotypes have greater tandem copy numbers of the genomic region encompassing the C-repeat binding factor genes Cbf2A and Cbf4B at Fr-H2 than the less freezing-tolerant nonwinter-hardy genotypes. Here we report that in wheat the Cbf14 gene at Fr-2 is copy number variable. Using DNA blot hybridizations, we estimated copy numbers of Cbf14 across the different genomes of diploid and polyploid wheat. Copy numbers of Cbf14 are lower in the B genome than in the A and D genomes across all ploidy levels. Among hexaploid red wheats, winter genotypes harbor greater Cbf14 copy numbers than spring genotypes. Cbf14 copy numbers also vary across the red winter wheats such that hard wheats harbor greater copy numbers than soft wheats. Analysis of hexaploid wheat chromosome 5 substitution lines indicates that Cbf14 copy numbers in the introgressions are stable in the different backgrounds. Taken together our data suggest that higher copy number states existed in the diploid wild ancestors prior to the polyploidization events and that the loss of Cbf14 copies occurred in the cultivated germplasm.
authors.affiliation: Department of Horticulture and Crop Science, The Ohio State University/Ohio Agricultural Research and Development Center (OARDC), Wooster, OH, 44691, USA.
authors.forename: Taniya
authors.initials: T
authors.lastname: Dhillon
authors.forename: Eric J
authors.initials: EJ
authors.lastname: Stockinger
mesh_terms: D032461:Chromosomes, Plant / Q000235:genetics; D056915:DNA Copy Number Variations / Q000235:genetics*; D004171:Diploidy; D017343:Genes, Plant / Q000235:genetics*; D018745:Genome, Plant / Q000235:genetics*; D006824:Hybridization, Genetic; D011123:Polyploidy; D014908:Triticum / Q000235:genetics*
references.citation: Plant Mol Biol. 2008 Jun;67(3):257-70
references.pmid: 18317935
references.citation: Annu Rev Plant Physiol Plant Mol Biol. 1999 Jun;50:571-599
references.pmid: 15012220
references.citation: Plant J. 1998 Nov;16(4):433-42
references.pmid: 9881163
references.citation: Genome. 2002 Dec;45(6):1095-106
references.pmid: 12502254
references.citation: Plant J. 2007 Jul;51(2):308-21
references.pmid: 17559507
references.citation: Science. 2004 Mar 12;303(5664):1640-4
references.pmid: 15016992
references.citation: Curr Opin Struct Biol. 2008 Jun;18(3):366-74
references.pmid: 18511261
references.citation: C R Biol. 2011 Mar;334(3):212-20
references.pmid: 21377616
references.citation: Theor Appl Genet. 1995 Jun;90(7-8):1174-9
references.pmid: 24173081
references.citation: Theor Appl Genet. 2006 May;112(8):1563-72
references.pmid: 16568284
references.citation: Plant J. 2003 Jan;33(2):373-83
references.pmid: 12535350
references.citation: Funct Integr Genomics. 2007 Jan;7(1):53-68
references.pmid: 16775685
references.citation: Annu Rev Med. 2010;61:437-55
references.pmid: 20059347
references.citation: Theor Appl Genet. 2004 Feb;108(4):670-80
references.pmid: 14576984
references.citation: Proc Natl Acad Sci U S A. 1997 Feb 4;94(3):1035-40
references.pmid: 9023378
references.citation: Theor Appl Genet. 2010 Jun;121(1):21-35
references.pmid: 20213518
references.citation: Plant Physiol. 2012 Aug;159(4):1295-308
references.pmid: 22696021
references.citation: Genetics. 2004 Oct;168(2):1087-96
references.pmid: 15514080
references.citation: Plant Mol Biol. 2005 Nov;59(4):533-51
references.pmid: 16244905
references.citation: PLoS One. 2012;7(3):e33234
references.pmid: 22457747
references.citation: Theor Appl Genet. 2007 Nov;115(8):1083-91
references.pmid: 17763839
references.citation: Mol Genet Genomics. 2005 Dec;274(5):506-14
references.pmid: 16200412
references.citation: Theor Appl Genet. 2005 Nov;111(8):1617-22
references.pmid: 16177898
references.citation: Theor Appl Genet. 2006 Mar;112(5):832-42
references.pmid: 16365758
references.citation: Proc Natl Acad Sci U S A. 2003 May 13;100(10):6263-8
references.pmid: 12730378
references.citation: Mol Genet Genomics. 2003 Apr;269(1):60-7
references.pmid: 12715154
references.citation: Plant Cell. 2005 Apr;17(4):1033-45
references.pmid: 15749759
references.citation: Genome Biol Evol. 2010 Jul 12;2:441-53
references.pmid: 20624746
references.citation: Theor Appl Genet. 2003 Aug;107(3):509-14
references.pmid: 12734655
references.citation: Curr Opin Plant Biol. 2009 Apr;12(2):178-84
references.pmid: 19195924
references.citation: Science. 2012 Nov 30;338(6111):1206-9
references.pmid: 23065905
references.citation: Mol Ecol. 2010 May;19(10):2075-88
references.pmid: 20406386
references.citation: Ann Bot. 2009 Jun;103(8):1165-72
references.pmid: 19304997
references.citation: Plant Physiol. 2010 Oct;154(2):571-7
references.pmid: 20921187
references.citation: J Hered. 1996 May-Jun;87(3):214-8
references.pmid: 8683097
references.citation: Genome. 1993 Feb;36(1):66-71
references.pmid: 18469970
references.citation: Plant Cell Physiol. 2011 May;52(5):750-64
references.pmid: 21317146
references.citation: Plant Cell. 1998 Aug;10(8):1391-406
references.pmid: 9707537
references.citation: Theor Appl Genet. 2007 Apr;114(6):947-59
references.pmid: 17318496
references.citation: Ann Bot. 2004 Nov;94(5):717-24
references.pmid: 15374834
references.citation: Plant Physiol. 2010 Aug;153(4):1846-58
references.pmid: 20571115
references.citation: Mol Genet Genomics. 2006 Feb;275(2):193-203
references.pmid: 16362370
references.citation: Genetics. 1988 Jun;119(2):453-6
references.pmid: 17246432