title: Influence of experimental canine ehrlichiosis on the E-ADA activity and purine levels in serum and possible functional correlations with pathogenesis.
abstract: The aim of this study was to evaluate adenosine deaminase activity and purines levels in serum of dogs experimentally infected by Ehrlichia canis. Banked serum samples of dogs divided into two groups with five animals each: healthy animals and animals infected by E. canis. The concentration of purines (adenosine triphosphate (ATP), adenosine diphosphate (ADP), adenosine monophosphate (AMP), adenosine, inosine, hypoxanthine, xanthine and uric acid), and adenosine deaminase (E-ADA) activity in sera were evaluated. Samples were collected on days 12 and 30 post-infection (PI). The E-ADA activity showed a significant reduction on day 12 PI, and increased on day 30 PI in dogs infected with E. canis. On day 12, an increase in seric concentration of ATP, ADP and adenosine was verified, and different levels of hypoxanthine, xanthine and uric acid had a drastic reduction in infected compared healthy dogs (P<0.05). However, on day 30 PI, the levels of seric ADP and AMP decreased, unlike the concentration of xanthine and uric acid that increased significantly in infected dogs (P<0.05). Therefore, the activity of E-ADA and purine levels are altered in experimental canine ehrlichiosis, probably with the purpose of modulating the pathogenesis of the disease related to immune response, oxidative stress and coagulation disorders in acute phase.
authors.affiliation: Department of Animal Science, Universidade do Estado de Santa Catarina (UDESC), Chapecó, SC, Brazil. aleksandro_ss@yahoo.com.br
authors.forename: Aleksandro S
authors.initials: AS
authors.lastname: Da Silva
authors.forename: Thiago D
authors.initials: TD
authors.lastname: Munhoz
authors.forename: Joice L M
authors.initials: JL
authors.lastname: Faria
authors.forename: Giovanni
authors.initials: G
authors.lastname: Vargas-Hérnandez
authors.forename: Rosangela Z
authors.initials: RZ
authors.lastname: Machado
authors.forename: Nathalia C
authors.initials: NC
authors.lastname: Luz
authors.forename: Cesar E J
authors.initials: CE
authors.lastname: Moritz
authors.forename: Emerson A
authors.initials: EA
authors.lastname: Casali
authors.forename: Nathieli B
authors.initials: NB
authors.lastname: Bottari
authors.forename: Lenita M
authors.initials: LM
authors.lastname: Stefani
authors.forename: Mirela
authors.initials: M
authors.lastname: Tinucci-Costa
mesh_terms: D000243:Adenosine Deaminase / Q000097:blood*; D000818:Animals; D004283:Dog Diseases / Q000097:blood / Q000201:enzymology* / Q000382:microbiology; D004285:Dogs; D041061:Ehrlichia canis / Q000472:pathogenicity / Q000502:physiology*; D016873:Ehrlichiosis / Q000097:blood / Q000201:enzymology / Q000382:microbiology / Q000662:veterinary*; D011687:Purines / Q000097:blood*; D014774:Virulence
chemical_list: D011687:Purines; D000243:Adenosine Deaminase; C030985:purine
keywords: Adenosine; Adenosine deaminase; Canine; Ehrlichia canis