title: Diagnostic value of sperm DNA fragmentation and sperm high-magnification for predicting outcome of assisted reproduction treatment.
abstract: Over the last years, major improvements in the field of male infertility diagnosis have been achieved. The aim of this study was to determine the diagnostic usefulness of sperm DNA integrity and sperm vacuolisation for predicting outcome in infertile couples undergoing in vitro fertilisation (IVF) and intracytoplasmic sperm injection (ICSI) treatments. A cohort study from 152 infertile couples undergoing sperm DNA fragmentation and high-magnification tests prior to an assisted reproduction treatment was designed. We found that the most predictive cutoff for pregnancy was 25.5% of DNA fragmentation with a negative predictive value of 72.7% (P=0.02). For the degree of vacuolisation, the best predictor of pregnancy was 73.5% of vacuolated sperm grades III+IV with a negative predictive value of 39.4% (P=0.09), which was not statistically significant. In conclusion, sperm DNA fragmentation greater than 25.5% could be associated with higher probability of failure IVF treatment. Regarding the results of the sperm analysis at high magnification, they do not allow us to predict whether or not patients will become pregnant.
authors.affiliation: Centro de Infertilidad y Reproducción Humana (CIRH), E-08017 Barcelona, Spain.
authors.forename: Gemma
authors.initials: G
authors.lastname: López
authors.forename: Rafael
authors.initials: R
authors.lastname: Lafuente
authors.forename: Miguel A
authors.initials: MA
authors.lastname: Checa
authors.forename: Ramón
authors.initials: R
authors.lastname: Carreras
authors.forename: Mario
authors.initials: M
authors.lastname: Brassesco
mesh_terms: D053938:DNA Fragmentation; D006801:Humans; D008297:Male; D027724:Reproductive Techniques, Assisted; D013094:Spermatozoa / Q000378:metabolism*
references.citation: Fertil Steril. 2008 Nov;90(5):1792-9
references.pmid: 18166175
references.citation: Reprod Biomed Online. 2008 Nov;17(5):610-6
references.pmid: 18983744
references.citation: J Androl. 2002 Jan-Feb;23(1):1-8
references.pmid: 11780915
references.citation: Fertil Steril. 2003 Jan;79(1):42-8
references.pmid: 12524062
references.citation: Fertil Steril. 2003 Oct;80(4):895-902
references.pmid: 14556809
references.citation: Fertil Steril. 2009 Sep;92(3):835-848
references.pmid: 19631936
references.citation: Int Braz J Urol. 2011 Jan-Feb;37(1):5-15
references.pmid: 21385475
references.citation: Reprod Biomed Online. 2010 Jan;20(1):132-7
references.pmid: 20158998
references.citation: Hum Reprod. 2000 Aug;15(8):1717-22
references.pmid: 10920092
references.citation: Fertil Steril. 1998 Aug;70(2):305-14
references.pmid: 9696226
references.citation: Reprod Biol Endocrinol. 2010 Jun 07;8:56
references.pmid: 20529256
references.citation: Asian J Androl. 2006 Jan;8(1):11-29
references.pmid: 16372115
references.citation: Hum Reprod. 2006 Mar;21(3):738-44
references.pmid: 16311292
references.citation: Folia Histochem Cytobiol. 2006;44(2):117-22
references.pmid: 16805137
references.citation: Fertil Steril. 2004 Aug;82(2):378-83
references.pmid: 15302287
references.citation: Hum Reprod. 2006 Jul;21(7):1787-90
references.pmid: 16497697
references.citation: Reprod Biomed Online. 2008 Jul;17(1):42-5
references.pmid: 18616888
references.citation: Reprod Biomed Online. 2010 Oct;21(4):450-5
references.pmid: 20800549
references.citation: Reprod Biomed Online. 2008 Nov;17(5):617-27
references.pmid: 18983745
references.citation: Fertil Steril. 2003 Dec;80(6):1413-9
references.pmid: 14667877
references.citation: Fertil Steril. 2009 Nov;92(5):1616-25
references.pmid: 18990390
references.citation: Reprod Biomed Online. 2008 Jun;16(6):835-41
references.pmid: 18549694
references.citation: Hum Reprod. 1997 May;12(5):987-93
references.pmid: 9194652
references.citation: Fertil Steril. 2005 Oct;84(4):833-42
references.pmid: 16213830
references.citation: Hum Reprod. 2003 May;18(5):1023-8
references.pmid: 12721180
references.citation: Reprod Biol. 2011 Jul;11(2):135-44
references.pmid: 21804634
references.citation: Reprod Biomed Online. 2012 Feb;24(2):211-8
references.pmid: 22227364
references.citation: Minerva Ginecol. 2003 Jun;55(3):233-9
references.pmid: 14581869
references.citation: Hum Reprod. 2011 Jan;26(1):47-58
references.pmid: 21088015
references.citation: Hum Reprod. 2005 Dec;20(12):3446-51
references.pmid: 16085665
references.citation: Hum Reprod. 2004 Jun;19(6):1401-8
references.pmid: 15117894
references.citation: Fertil Steril. 2008 Apr;89(4):823-31
references.pmid: 17644094
references.citation: Hum Reprod. 2010 Jul;25(7):1594-608
references.pmid: 20447937
references.citation: J Androl. 2003 Jan-Feb;24(1):59-66
references.pmid: 12514084