title: Clinicopathological significance of SLP-2 overexpression in human gallbladder cancer.
abstract: Several studies have indicated that overexpression of stomatin-like protein 2 (SLP-2) has been identified in several types of cancer. However, its role and clinical relevance in gallbladder cancer (GBC) is unknown. The purpose of this study was to reveal the prognostic significance of SLP-2 in GBC. The SLP-2 expression was examined at mRNA and protein levels by real-time quantitative polymerase chain reaction (qRT-PCR), and immunohistochemistry in GBC tissues and adjacent noncancerous tissues. Statistical analyses were applied to test the associations between SLP-2 expression, clinicopathologic factors, and prognosis. Immunohistochemistry and qRT-PCR showed that the protein and mRNA expression levels of SLP-2 were both significantly higher in GBC tissues than in adjacent noncancerous tissues. In addition, immunohistochemistry analysis showed that SLP-2 expression was significantly correlated with histological grade (P <0.001), pathologic T stage (P = 0.019), clinical stage (P = 0.001), and lymph node metastasis (P = 0.026). The Kaplan-Meier survival curves indicated that patients with high expression of SLP-2 had shorter overall survival than those with low expression (P <0.001). Meanwhile, the Cox multivariate analysis indicated that high expressions of SLP-2 were an independent prognostic factor for patients with GBC. These data showed that SLP-2 may play an important role in human GBC tumorigenesis, and SLP-2 might serve as a novel prognostic marker in human GBC.
authors.affiliation: Department of General Surgery, The Affiliated People's Hospital of Jiangsu University, No. 8, Dianli Road, Zhenjiang, 212002, China.
authors.forename: Wei-Xin
authors.initials: WX
authors.lastname: Wang
authors.forename: Qing-Feng
authors.initials: QF
authors.lastname: Lin
authors.forename: Dong
authors.initials: D
authors.lastname: Shen
authors.forename: Shao-Ping
authors.initials: SP
authors.lastname: Liu
authors.forename: Wei-Dong
authors.initials: WD
authors.lastname: Mao
authors.forename: Gui
authors.initials: G
authors.lastname: Ma
authors.forename: Wei-Dong
authors.initials: WD
authors.lastname: Qi
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D001798:Blood Proteins / Q000235:genetics* / Q000378:metabolism; D005260:Female; D005706:Gallbladder Neoplasms / Q000235:genetics* / Q000401:mortality / Q000473:pathology*; D015870:Gene Expression; D006801:Humans; D008297:Male; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D008875:Middle Aged; D060787:Neoplasm Grading; D009362:Neoplasm Metastasis; D009367:Neoplasm Staging; D012306:Risk; D047368:Tumor Burden
chemical_list: D001798:Blood Proteins; D008565:Membrane Proteins; C406287:STOML2 protein, human
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