title: Measurement of lecithin-cholesterol acyltransferase activity with the use of a Peptide-proteoliposome substrate.
abstract: Lecithin-cholesterol acyltransferase (LCAT) is the major enzyme responsible for the esterification of free cholesterol on plasma lipoproteins, which is a key step in the reverse cholesterol transport pathway. The measurement of plasma LCAT activity not only is important in the diagnosis of patients with genetic or acquired LCAT deficiency but is also valuable in calculating cardiovascular risk, as well as in research studies of lipoprotein metabolism. In this chapter, we describe a convenient LCAT assay based on the use of an apoA-I mimetic peptide. The proteoliposome substrate used in this assay for LCAT is easily made with the peptide and can be stored by deep freezing without significant loss of activity.
authors.affiliation: Lipoprotein Metabolism Section, Cardiovascular-Pulmonary Branch, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD, USA.
authors.forename: Boris L
authors.initials: BL
authors.lastname: Vaisman
authors.forename: Alan T
authors.initials: AT
authors.lastname: Remaley
mesh_terms: D016632:Apolipoprotein A-I / Q000737:chemistry* / Q000378:metabolism; D002318:Cardiovascular Diseases / Q000097:blood / Q000201:enzymology*; D002784:Cholesterol / Q000097:blood*; D002788:Cholesterol Esters / Q000097:blood; D006801:Humans; D054709:Lecithins / Q000097:blood; D007862:Phosphatidylcholine-Sterol O-Acyltransferase / Q000097:blood*; D011510:Proteolipids / Q000737:chemistry / Q000235:genetics; D013379:Substrate Specificity
chemical_list: D016632:Apolipoprotein A-I; D002788:Cholesterol Esters; D054709:Lecithins; D011510:Proteolipids; C020485:proteoliposomes; D002784:Cholesterol; D007862:Phosphatidylcholine-Sterol O-Acyltransferase