title: The properties of chitosan complexes with smooth and rough forms of lipopolysaccharides on CHO-K1 cells.
abstract: The negative charge of LPS molecule and the presence of fatty acids in lipid A structure make it capable of binding with chitosan. In the presented work we analyzed the interactions of chitosan with LPS of Burkholderia cepacia or Proteus mirabilis and biological effects of these complexes on CHO-K1 cells. We observed that the presence of O-polysaccharide part of LPS (S1959), core region (R110) or lack of fatty acids in lipid A increased binding affinity of endotoxin with chitosan. However, lipid A of B. cepacia or P. mirabilis R45 might interact with CHO-K1 cells membrane alone or mediated by chitosan, respectively. In conclusion, the presence of two (B. cepacia) or one (P. mirabilis R45) Ara4N residues in lipid A part, promoted binding to cell membrane of CHO-K1 cells, alone or in the presence of chitosan, respectively. Chitosan reduced biological potencies of P. mirabilis lipid A R45 structure and this effect depended on the presence of O-PS. Lipid A of B. cepacia induced oxidative DNA damage in CHO-K1 cells.
authors.affiliation: Department of Microbiology, Jan Kochanowski University, Świętokrzyska 15, 25-406 Kielce, Poland. arabski@ujk.kielce.pl
authors.forename: Michał
authors.initials: M
authors.lastname: Arabski
authors.forename: Halina
authors.initials: H
authors.lastname: Lisowska
authors.forename: Anna
authors.initials: A
authors.lastname: Lankoff
authors.forename: Viktoria N
authors.initials: VN
authors.lastname: Davydova
authors.forename: Zuzanna
authors.initials: Z
authors.lastname: Drulis-Kawa
authors.forename: Daria
authors.initials: D
authors.lastname: Augustyniak
authors.forename: Irina M
authors.initials: IM
authors.lastname: Yermak
authors.forename: Antonio
authors.initials: A
authors.lastname: Molinaro
authors.forename: Wiesław
authors.initials: W
authors.lastname: Kaca
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D019117:Burkholderia / Q000737:chemistry; D016466:CHO Cells; D011232:Chemical Precipitation; D048271:Chitosan / Q000494:pharmacology*; D003091:Colistin / Q000378:metabolism; D003167:Complement Activation / Q000187:drug effects; D006224:Cricetinae; D003412:Cricetulus; D004249:DNA Damage; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007700:Kinetics; D008070:Lipopolysaccharides / Q000737:chemistry* / Q000494:pharmacology*; D009153:Mutagens / Q000633:toxicity; D011513:Proteus mirabilis / Q000737:chemistry
chemical_list: D008070:Lipopolysaccharides; D009153:Mutagens; D048271:Chitosan; D003091:Colistin
keywords: Bulkhorderia; CHO-K1 cells; Chitosan; Comet assay; Lipopolysaccharide; Proteus