title: Localization and expression of Hsp27 and αB-crystallin in rat primary myocardial cells during heat stress in vitro.
abstract: Neonatal rat primary myocardial cells were subjected to heat stress in vitro, as a model for investigating the distribution and expression of Hsp27 and αB-crystallin. After exposure to heat stress at 42°C for different durations, the activities of enzymes expressed during cell damage increased in the supernatant of the heat-stressed myocardial cells from 10 min, and the pathological lesions were characterized by karyopyknosis and acute degeneration. Thus, cell damage was induced at the onset of heat stress. Immunofluorescence analysis showed stronger positive signals for both Hsp27 and αB-crystallin from 10 min to 240 min of exposure compared to the control cells. According to the Western blotting results, during the 480 min of heat stress, no significant variation was found in Hsp27 and αB-crystallin expression; however, significant differences were found in the induction of their corresponding mRNAs. The expression of these small heat shock proteins (sHsps) was probably delayed or overtaxed due to the rapid consumption of sHsps in myocardial cells at the onset of heat stress. Our findings indicate that Hsp27 and αB-crystallin do play a role in the response of cardiac cells to heat stress, but the details of their function remain to be investigated.
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China.
authors.forename: Shu
authors.initials: S
authors.lastname: Tang
authors.forename: Rehana
authors.initials: R
authors.lastname: Buriro
authors.forename: Zhijun
authors.initials: Z
authors.lastname: Liu
authors.forename: Miao
authors.initials: M
authors.lastname: Zhang
authors.forename: Islam
authors.initials: I
authors.lastname: Ali
authors.forename: Abdelnasir
authors.initials: A
authors.lastname: Adam
authors.forename: Jörg
authors.initials: J
authors.lastname: Hartung
authors.forename: Endong
authors.initials: E
authors.lastname: Bao
mesh_terms: D000818:Animals; D052279:Creatine Kinase, MB Form / Q000378:metabolism; D004789:Enzyme Activation; D015870:Gene Expression; D005786:Gene Expression Regulation; D055551:HSP27 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism*; D006358:Hot Temperature; D032383:Myocytes, Cardiac / Q000201:enzymology / Q000378:metabolism* / Q000473:pathology; D021381:Protein Transport; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D013312:Stress, Physiological; D038203:alpha-Crystallin B Chain / Q000235:genetics / Q000378:metabolism*
chemical_list: D055551:HSP27 Heat-Shock Proteins; D012333:RNA, Messenger; D038203:alpha-Crystallin B Chain; D052279:Creatine Kinase, MB Form
references.citation: Int J Hyperthermia. 2004 Mar;20(2):115-30
references.pmid: 15195506
references.citation: Crit Rev Oncol Hematol. 2002 Jul;43(1):33-56
references.pmid: 12098606
references.citation: Adv Protein Chem. 2001;59:105-56
references.pmid: 11868270
references.citation: J Forensic Sci. 2011 Jul;56(4):1062-3
references.pmid: 21470226
references.citation: Biochem Cell Biol. 2008 Dec;86(6):521-38
references.pmid: 19088800
references.citation: Hum Exp Toxicol. 2004 May;23(5):251-6
references.pmid: 15222403
references.citation: Cell. 1989 Apr 7;57(1):71-8
references.pmid: 2539261
references.citation: Dev Genet. 1993;14(2):103-11
references.pmid: 8482014
references.citation: Annu Rev Cell Biol. 1993;9:601-34
references.pmid: 8280473
references.citation: Endocr Res. 2005;31(3):171-5
references.pmid: 16392619
references.citation: Annu Rev Biochem. 1986;55:1151-91
references.pmid: 2427013
references.citation: Res Vet Sci. 1995 Jul;59(1):30-4
references.pmid: 8525081
references.citation: Nature. 1998 Aug 6;394(6693):595-9
references.pmid: 9707123
references.citation: Cell Stress Chaperones. 2009 Jan;14(1):61-9
references.pmid: 18548335
references.citation: Cell. 1998 Feb 6;92(3):351-66
references.pmid: 9476895
references.citation: Shock. 1999 Jan;11(1):1-12
references.pmid: 9921710
references.citation: Biochem Pharmacol. 2000 Jan 1;59(1):55-63
references.pmid: 10605935
references.citation: Res Vet Sci. 2011 Jun;90(3):432-8
references.pmid: 20659751
references.citation: Fa Yi Xue Za Zhi. 2006 Feb;22(1):21-3
references.pmid: 16524179
references.citation: Cell Mol Life Sci. 2002 Oct;59(10):1649-57
references.pmid: 12475175
references.citation: Biochem Biophys Res Commun. 1989 Jan 16;158(1):319-25
references.pmid: 2912453
references.citation: Cell Stress Chaperones. 2008 Dec;13(4):459-66
references.pmid: 18465207
references.citation: J Neurochem. 2002 Oct;83(2):452-62
references.pmid: 12423255
references.citation: Int J Biochem Cell Biol. 2012 Oct;44(10):1588-92
references.pmid: 22449631
references.citation: Circulation. 2005 Nov 29;112(22):3451-61
references.pmid: 16316967
references.citation: Curr Protoc Immunol. 2004 Feb;Appendix 1:Appendix 1T
references.pmid: 18432918
references.citation: Am J Physiol. 1998 Dec;275(6):H2243-9
references.pmid: 9843825
references.citation: Phytomedicine. 2010 Dec 15;18(1):52-7
references.pmid: 20638259
references.citation: Biochim Biophys Acta. 2006 Feb;1762(2):164-80
references.pmid: 16236486
references.citation: J Vet Sci. 2012 Sep;13(3):253-9
references.pmid: 23000582
references.citation: J Biol Chem. 2000 Jan 14;275(2):1095-104
references.pmid: 10625651
references.citation: Eur Respir J. 1993 Apr;6(4):483-8
references.pmid: 8491297
references.citation: Antioxid Redox Signal. 2005 Mar-Apr;7(3-4):404-13
references.pmid: 15706087
references.citation: Int J Biochem Cell Biol. 2012 Oct;44(10):1680-6
references.pmid: 22683760
references.citation: Cancer Res. 1996 Jan 15;56(2):273-9
references.pmid: 8542580
references.citation: Int J Biochem Cell Biol. 2012 Oct;44(10):1632-45
references.pmid: 22710345
references.citation: Br Med J (Clin Res Ed). 1986 Oct 11;293(6552):917-22
references.pmid: 3094714
references.citation: Int J Biochem Cell Biol. 2012 Oct;44(10):1622-31
references.pmid: 22521623
references.citation: Nat Struct Mol Biol. 2005 Oct;12(10):842-6
references.pmid: 16205709
references.citation: Ren Fail. 2001 Sep;23(5):727-30
references.pmid: 11725920
references.citation: EMBO J. 1996 Jun 3;15(11):2695-706
references.pmid: 8654367
references.citation: Anticancer Res. 2010 Jul;30(7):2539-43
references.pmid: 20682980
references.citation: Nature. 2012 Oct 11;490(7419):213-8
references.pmid: 22972192
references.citation: J Surg Res. 2001 Aug;99(2):328-34
references.pmid: 11469906
references.citation: FASEB J. 2001 Feb;15(2):393-402
references.pmid: 11156955
references.citation: Int J Cardiol. 2013 May 25;165(3):494-8
references.pmid: 21962802
references.citation: Mol Cell Biol. 1995 Jan;15(1):505-16
references.pmid: 7799959
references.citation: J Biol Chem. 2002 May 31;277(22):19913-21
references.pmid: 11912188
references.citation: Int J Biochem Cell Biol. 2012 Oct;44(10):1587
references.pmid: 22743309
references.citation: Cell Stress Chaperones. 2008 Sep;13(3):327-35
references.pmid: 18350374