title: Effects of magnolialide isolated from the leaves of Laurus nobilis L. (Lauraceae) on immunoglobulin E-mediated type I hypersensitivity in vitro.
abstract: BACKGROUND
  Laurus nobilis L. (Lauraceae) has been used for folk medicines in the Mediterranean area and Europe to treat various disorders including skin inflammation (dermatitis) and asthma.
  
  
  OBJECTIVE
  Our aim was to investigate the scientific evaluation of the compounds from Laurus nobilis L. on immuniglobulin E (IgE)-mediated type I hypersensitivity responses in vitro such as atopic dermatitis and asthma.
  
  
  METHODS
  Seven compounds were isolated and examined for the mast cell stabilizing effect on IgE-sensitized RBL-2H3 mast cells by measuring the β-hexosaminidase activity. In addition, the effects on interleukin (IL)-4 production and IL-5-dependent Y16 early B cell proliferation were investigated as well as their cytotoxic effects on RBL-2H3 cells.
  
  
  RESULTS
  Among the seven isolated compounds, magnolialide attenuated the release of β-hexosaminidase from RBL-2H3 cells with an IC50 value of 20.2 μM, while the other compounds revealed no significant effects at concentrations tested. Furthermore, magnolialide significantly inhibited the IL-4 release with an IC50 value of 18.1 μM and IL-4 mRNA expression with an IC50 value of 15.7 μM in IgE-sensitized RBL-2H3 cells. In addition, the inhibition of IL-5-dependent proliferation of early B cells (Y16 cells) by magnolialide was demonstrated with an IC50 value of 18.4 μM.
  
  
  CONCLUSIONS
  These results suggest that the magnolialide might be a candidate for the treatment of IgE-mediated hypersensitivity responses such as atopic dermatitis and asthma by inhibiting mast cell degranulation, the IL-4 production, and IL-5-dependent early B cell proliferation, key factors in the development and amplification of type I hypersensitivity reactions.
authors.affiliation: Natural Products Research Institute, College of Pharmacy, Seoul National University, Gwanak-gu, Seoul, Republic of Korea.
authors.forename: Taehun
authors.initials: T
authors.lastname: Lee
authors.forename: Sooryun
authors.initials: S
authors.lastname: Lee
authors.forename: Kyeong
authors.initials: K
authors.lastname: Ho Kim
authors.forename: Ki-Bong
authors.initials: KB
authors.lastname: Oh
authors.forename: Jongheon
authors.initials: J
authors.lastname: Shin
authors.forename: Woongchon
authors.initials: W
authors.lastname: Mar
mesh_terms: D000818:Animals; D018926:Anti-Allergic Agents / Q000494:pharmacology*; D002460:Cell Line; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004140:Dinitrophenols / Q000276:immunology; D006241:Haptens / Q000276:immunology; D006967:Hypersensitivity / Q000276:immunology; D007073:Immunoglobulin E / Q000276:immunology; D015847:Interleukin-4 / Q000235:genetics / Q000378:metabolism; D007783:Lactones / Q000494:pharmacology*; D027442:Laurus; D051379:Mice; D018515:Plant Leaves; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D012709:Serum Albumin / Q000276:immunology; D012717:Sesquiterpenes / Q000494:pharmacology*; D001619:beta-N-Acetylhexosaminidases / Q000378:metabolism
chemical_list: D018926:Anti-Allergic Agents; D004140:Dinitrophenols; D006241:Haptens; D007783:Lactones; D012333:RNA, Messenger; D012709:Serum Albumin; D012717:Sesquiterpenes; C058064:dinitrophenyl-human serum albumin conjugate; C417229:magnolialide; D015847:Interleukin-4; D007073:Immunoglobulin E; D001619:beta-N-Acetylhexosaminidases
keywords: Interleukin-4; Interleukin-5; Lauraceae; Laurus nobilis; Magnolialide; β-hexosaminidase