title: Predominance of bovine viral diarrhea virus 1b and 1d subtypes during eight years of survey in Poland.
abstract: The genetic diversity of bovine viral diarrhea virus (BVDV) was determined from 65 animals persistently infected with BVDV and diagnosed between 2004 and 2011 in Poland. The samples originated from 28 herds in 12 provinces, where over 90% of the whole cattle population of Poland is reared. Phylogenetic analysis based on the fragments of two genomic regions of BVDV namely, 5'-untranslated region (5'-UTR) and N(pro) was performed. All the BVDV isolates belonged to BVDV-1 species and were further divided into four subtypes. There were 31 viruses of BVDV-1b subtype (47.6%) present in 12 herds, 24 of BVDV-1d subtype (36.9%) in 9 herds, 8 of BVDV-1f subtype (12.3%) in 5 herds and 2 BVDV-1g subtype (3.0%) in 2 herds. Neither BVDV-1a subtype, nor BVDV-2 species or any atypical bovine pestivirus were found among isolates tested. Despite increasing import of live cattle in the recent years, genetic diversity of Polish BVDV isolates was rather low.
authors.affiliation: National Veterinary Research Institute, Department of Virology, Al. Partyzantów 57, 24-100 Puławy, Poland.
authors.forename: A
authors.initials: A
authors.lastname: Kuta
authors.forename: M P
authors.initials: MP
authors.lastname: Polak
authors.forename: M
authors.initials: M
authors.lastname: Larska
authors.forename: J F
authors.initials: JF
authors.lastname: Żmudziński
mesh_terms: D020121:5' Untranslated Regions; D000818:Animals; D001483:Base Sequence; D001912:Bovine Virus Diarrhea-Mucosal Disease / Q000453:epidemiology / Q000235:genetics / Q000821:virology*; D002417:Cattle; D030241:Diarrhea Virus 1, Bovine Viral / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D014644:Genetic Variation; D008969:Molecular Sequence Data; D010802:Phylogeny; D011044:Poland / Q000453:epidemiology
chemical_list: D020121:5' Untranslated Regions
keywords: BVDV; Bovine viral diarrhea virus; Cattle; Genotyping; Pestivirus