title: Assessment of association between variants and haplotypes of the IGF2 gene in beef cattle.
abstract: Insulin-like growth factor 2 (IGF2) is a fetal growth and differentiation factor that plays an important role in muscle growth and in myoblast proliferation and differentiation. The aim of this study was to examine the association of the IGF2 polymorphism with growth traits in beef cattle breed. Four single nucleotide polymorphisms (SNPs: 1-4) were identified in the bovine IGF2 by sequencing pooled DNA samples (Pool-Seq) and forced polymerase chain reaction-restriction fragment length polymorphism (Forced PCR-RFLP) methods. The result of haplotype analysis of four SNPs showed that eight haplotypes and eighteen combined genotypes were revealed, and the linkage disequilibrium and evolutionary relationship were assessed in 1522 individuals representing four purebred cattle breeds from China. The statistical analyses indicated that the 4 SNPs and 18 combined genotypes or haplotypes are associated with the body weight at 18 and 24 months in Jiaxian cattle population (P<0.05 or P<0.01). Our results provide evidence that polymorphisms in the IGF2 gene are associated with growth traits, and may be used for marker-assisted selection in beef cattle breeding program.
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling, Shaanxi 712100, China. huangyongzhen2009@yahoo.com.cn
authors.forename: Yong-Zhen
authors.initials: YZ
authors.lastname: Huang
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.forename: Zhao-Yang
authors.initials: ZY
authors.lastname: Zhan
authors.forename: Xiu-Kai
authors.initials: XK
authors.lastname: Cao
authors.forename: Yu-Jia
authors.initials: YJ
authors.lastname: Sun
authors.forename: Xian-Yong
authors.initials: XY
authors.lastname: Lan
authors.forename: Chu-Zhao
authors.initials: CZ
authors.lastname: Lei
authors.forename: Chun-Lei
authors.initials: CL
authors.lastname: Zhang
authors.forename: Hong
authors.initials: H
authors.lastname: Chen
mesh_terms: D000818:Animals; D001947:Breeding; D002417:Cattle / Q000235:genetics* / Q000254:growth & development; D005787:Gene Frequency; D056726:Genetic Association Studies; D006239:Haplotypes; D007335:Insulin-Like Growth Factor II / Q000235:genetics*; D015810:Linkage Disequilibrium; D018482:Muscle, Skeletal / Q000254:growth & development; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D017422:Sequence Analysis, DNA
chemical_list: D007335:Insulin-Like Growth Factor II
keywords: Association analysis; BW; CH; Cattle; Chinese Holstein; GLM; Growth trait; HWE; Haplotype; Hardy–Weinberg equilibrium; He; IGF2; Insulin-like growth factor 2; JX; Jiaxian; LD; LSM; NY; Nanyang; Ne; PIC; Polymorphism information content; QC; QTL; Qinchuan; SNPs; SPSS; base pair(s); body weight; bp; effective allele numbers; general linear models; heterozygosity; least square means estimates; linkage disequilibrium; quantitative trait locus; single nucleotide polymorphisms; statistical product and service solutions