title: Identification of a protective protein from stationary-phase exoproteome of Brucella abortus.
abstract: Brucellosis is a worldwide zoonotic disease. No Brucella vaccine is available for use in humans, and existing animal vaccines have limitations. To search the putative vaccine candidates, we studied the exoproteome of Brucella abortus NCTC 10093 using 2-DE-MS approach. Twenty-six proteins were identified using MALDI-TOF/TOF tandem mass spectrometry. Outer membrane protein 25, d-galactose periplasmic-binding protein, oligopeptide ABC transporter protein and isopropylmalate synthase were found to be the most abundant proteins. Most proteins (6, 23%) were predicted to be involved in amino acid transport and metabolism followed by carbohydrate transport and metabolism (4, 15%). Outer membrane protein 25, Omp2b porin and one hypothetical protein were predicted as outer membrane proteins. In addition, Omp28, Omp31 and one ribosomal protein (L9) were also identified. The ribosomal protein L9 was produced as a recombinant protein and was studied in mouse model for vaccine potential. It was found to be immunogenic in terms of generating serum antibody response and release of IFN-γ from mice spleen cells. Recombinant L9-immunized mice were protected against challenge with virulent B. abortus strain 544, suggesting usefulness of ribosomal protein L9 as a good vaccine candidate against brucellosis.
authors.affiliation: Division of Microbiology, Defence Research & Development Establishment, Gwalior, Madhya Pradesh, India.
authors.forename: Shikha
authors.initials: S
authors.lastname: Jain
authors.forename: Subodh
authors.initials: S
authors.lastname: Kumar
authors.forename: Sudhir
authors.initials: S
authors.lastname: Dohre
authors.forename: Prachiti
authors.initials: P
authors.lastname: Afley
authors.forename: Nabonita
authors.initials: N
authors.lastname: Sengupta
authors.forename: Syed I
authors.initials: SI
authors.lastname: Alam
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology; D001426:Bacterial Proteins / Q000276:immunology* / Q000378:metabolism*; D002004:Brucella Vaccine / Q000276:immunology; D002003:Brucella abortus / Q000276:immunology* / Q000378:metabolism*; D002006:Brucellosis / Q000276:immunology / Q000378:metabolism / Q000517:prevention & control; D005260:Female; D007371:Interferon-gamma / Q000276:immunology / Q000378:metabolism; D008565:Membrane Proteins / Q000276:immunology / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D020543:Proteome / Q000276:immunology* / Q000378:metabolism*; D011994:Recombinant Proteins / Q000276:immunology / Q000378:metabolism; D012269:Ribosomal Proteins / Q000276:immunology / Q000378:metabolism; D013154:Spleen / Q000276:immunology / Q000378:metabolism
chemical_list: D000907:Antibodies, Bacterial; D001426:Bacterial Proteins; D002004:Brucella Vaccine; D008565:Membrane Proteins; D020543:Proteome; D011994:Recombinant Proteins; D012269:Ribosomal Proteins; C049650:ribosomal protein L9; D007371:Interferon-gamma
keywords: Brucella abortus; L9 protein; exoproteome; vaccine candidate