title: Analysis of unfolded protein response in Arabidopsis.
abstract: The unfolded protein response (UPR) is fundamental for development and adaption in eukaryotic cells. Arabidopsis has become one of the best model systems to uncover conserved mechanisms of the UPR in multicellular eukaryotes as well as organism-specific regulation of the UPR in plants. Monitoring the UPR in planta is an elemental approach to identifying regulatory components and to revealing molecular mechanisms of the plant UPR. In this chapter, we provide protocols for the induction and analyses of plant UPR at a molecular level in Arabidopsis. Three kinds of ER stress treatment methods and quantitation of the plant UPR activation are described here.
authors.affiliation: Department of Energy Plant Research Laboratory, Michigan State University, East Lansing, MI, USA.
authors.forename: Yani
authors.initials: Y
authors.lastname: Chen
authors.forename: Federica
authors.initials: F
authors.lastname: Brandizzi
mesh_terms: D017360:Arabidopsis / Q000235:genetics*; D004721:Endoplasmic Reticulum / Q000235:genetics / Q000378:metabolism; D059865:Endoplasmic Reticulum Stress; D018506:Gene Expression Regulation, Plant; D056811:Unfolded Protein Response / Q000235:genetics*
references.citation: Mol Cell. 2007 Jul 6;27(1):53-66
references.pmid: 17612490
references.citation: Biochemistry. 1979 Sep 4;18(18):3946-52
references.pmid: 486403
references.citation: Nature. 1988 Mar 31;332(6163):462-4
references.pmid: 3352747
references.citation: Cell. 1996 Nov 1;87(3):391-404
references.pmid: 8898193
references.citation: Antimicrob Agents Chemother. 1996 Jul;40(7):1640-4
references.pmid: 8807054
references.citation: Mol Genet Genomics. 2008 Sep;280(3):199-210
references.pmid: 18574595
references.citation: Plant J. 2012 Jan;69(2):266-77
references.pmid: 21914012
references.citation: Methods. 2005 Apr;35(4):395-416
references.pmid: 15804613
references.citation: Biochemistry. 1979 May 29;18(11):2186-92
references.pmid: 444447
references.citation: Proc Natl Acad Sci U S A. 2005 Apr 5;102(14):5280-5
references.pmid: 15781873
references.citation: J Antibiot (Tokyo). 1971 Apr;24(4):215-23
references.pmid: 5572750