title: In vitro and in vivo evaluation of chitosan/β-glycerol phosphate composite membrane for guided bone regeneration.
abstract: Chitosan and β-glycerol phosphate (CS/β-GP) composite, with a thermosensitive sol-gel transition behavior, has been tested as one of the viable materials for barrier membrane fabrication. These studies have provided us with a new concept for a guided bone regeneration (GBR) membrane design. The composition, porous structure of the membrane, and the neutral mild preparation procedures make the CS/β-GP membrane a potentially active guide for bone regeneration. In this study, the CS/β-GP composite membrane, with different concentrations of β-GP, was studied to assess their potential utility in GBR application. The initial attachment of the ST2 stromal cell line to the CS/β-GP composite membrane was better than their attachment to the pure CS membrane. The proliferation and osteoblastic differentiation of the cells were much higher on the CS/β-GP composite membrane as compared to the pure CS membrane (p < 0.05). A mild inflammatory response was observed around the implanted CS/β-GP composite membrane without any foreign body reaction that continued up to 4 weeks of postsurgery. This primary study indicated that the in vitro and in vivo bioactivities of the CS/β-GP composite membrane fulfilled the requirements for GBR technique.
authors.affiliation: Department of Dental Implantology, School of Stomatology, Shandong University, Shandong Province, Jinan, 250012, People's Republic of China; Department of Dental Implantology, Jinan Stomatological Hospital, Shandong Province, Jinan, 250001, People's Republic of China.
authors.forename: Jun
authors.initials: J
authors.lastname: Cui
authors.forename: Jie
authors.initials: J
authors.lastname: Liang
authors.forename: Yong
authors.initials: Y
authors.lastname: Wen
authors.forename: Xiaoning
authors.initials: X
authors.lastname: Sun
authors.forename: Tiejun
authors.initials: T
authors.lastname: Li
authors.forename: Gairong
authors.initials: G
authors.lastname: Zhang
authors.forename: Kangning
authors.initials: K
authors.lastname: Sun
authors.forename: Xin
authors.initials: X
authors.lastname: Xu
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry* / Q000378:metabolism; D001861:Bone Regeneration; D002460:Cell Line; D048271:Chitosan / Q000737:chemistry* / Q000378:metabolism; D005260:Female; D005994:Glycerophosphates / Q000737:chemistry* / Q000378:metabolism; D048091:Guided Tissue Regeneration / Q000379:methods*; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry* / Q000378:metabolism; D008422:Materials Testing; D008567:Membranes, Artificial; D051379:Mice; D044367:Phase Transition; D019736:Prostheses and Implants; D051381:Rats; D017208:Rats, Wistar; D013696:Temperature
chemical_list: D001672:Biocompatible Materials; D005994:Glycerophosphates; D008567:Membranes, Artificial; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D048271:Chitosan; C031463:beta-glycerophosphoric acid
keywords: barrier membrane; chitosan; guided bone regeneration; thermo-sensitive hydrogel; β-glycerol phosphate