title: Chemiluminescence determination of streptomycin in pharmaceutical preparation and its application to pharmacokinetic study by a flow injection analysis assembly.
abstract: A novel and rapid method for the determination of streptomycin has been established by chemiluminescence (CL) based on significant intensity enhancement of streptomycin on the weak CL of N-bromosuccinimide (NBS) and eosin in alkaline medium. The method is simple, rapid and effective to determine streptomycin in the range of 8.0×10(-9)-1.0×10(-6)gmL(-1) with a determination limit of 2.25×10(-9)gmL(-1). The relative standard deviation is 1.95% for the determination of 2.0×10(-7)gmL(-1) streptomycin (n=11). The pharmacokinetics of streptomycin in plasma of rat coincides with the two-compartment open model. The T1/2α, T1/2β, CL/F, AUC(0-t), MRT, Tmax and Cmax were 18.83±1.24min, 82.14±3.07min, 0.0026±0.0011Lkg(-1)min(-1), 36044.50±105.02mgmin(-1)L(-1), 92.29±8.21min, 21.63±1.26min and 375.61±8.50μgmL(-1), respectively. There was no significant difference between the results obtained by CL and HPLC. The FI-CL method can be used to determine streptomycin in pharmaceutical preparation and biological samples. The established method is simple, rapid and sensitive without expensive instruments. The possible enhancement mechanism was also investigated.
authors.affiliation: School of Pharmaceutical Science, Zhengzhou University, Zhengzhou 450001, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Du
authors.forename: Hongyan
authors.initials: H
authors.lastname: Li
authors.forename: Jianwen
authors.initials: J
authors.lastname: Jin
authors.forename: Tiantian
authors.initials: T
authors.lastname: Wang
authors.forename: Yang
authors.initials: Y
authors.lastname: Li
authors.forename: Guopeng
authors.initials: G
authors.lastname: Shen
authors.forename: Xiaotian
authors.initials: X
authors.lastname: Li
mesh_terms: D000818:Animals; D001974:Bromosuccinimide / Q000737:chemistry; D004801:Eosine Yellowish-(YS) / Q000737:chemistry; D017022:Flow Injection Analysis / Q000295:instrumentation / Q000379:methods*; D007700:Kinetics; D008163:Luminescent Measurements / Q000295:instrumentation / Q000379:methods*; D004364:Pharmaceutical Preparations / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013056:Spectrophotometry, Ultraviolet; D013307:Streptomycin / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics*
chemical_list: D004364:Pharmaceutical Preparations; D001974:Bromosuccinimide; D004801:Eosine Yellowish-(YS); D013307:Streptomycin
keywords: Chemiluminescence; Eosin; Pharmacokinetics; Streptomycin sulfate