title: An open tubular capillary electrochromatography column with porous inner surface for protein separation.
abstract: An open tubular capillary electrochromatography (OTCEC) column using sole porogen to form porous inner surface has been developed. The porous layer was coated on the capillary inner wall by in situ polymerization in the presence of porogen. The results show that the columns using 1-propanol as sole porogen are appropriate for protein separation. It has higher separation efficiency than the column with the usual coporogen due to much more micropores and mesopores on the porous surface and a higher specific surface area. In addition, the sensitivity of the prepared OTCEC column was improved greatly compared with the dynamically coated capillary with polyvinylpyrrolidone.
authors.affiliation: School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai 200237, People's Republic of China. Electronic address: hyliu@ecust.edu.cn.
authors.forename: Haiyan
authors.initials: H
authors.lastname: Liu
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Li
authors.forename: Lanqi
authors.initials: L
authors.lastname: Huang
authors.forename: Lingyi
authors.initials: L
authors.lastname: Zhang
authors.forename: Weibing
authors.initials: W
authors.lastname: Zhang
mesh_terms: D000818:Animals; D053801:Capillary Electrochromatography / Q000379:methods*; D002417:Cattle; D008689:Methacrylates / Q000737:chemistry; D011108:Polymers / Q000737:chemistry; D016062:Porosity; D011506:Proteins / Q000302:isolation & purification*; D012821:Silanes / Q000737:chemistry; D013499:Surface Properties
chemical_list: D008689:Methacrylates; D011108:Polymers; D011506:Proteins; D012821:Silanes; C017492:methacryloxypropyltrimethoxysilane; C004919:ethylene dimethacrylate; C016284:butyl methacrylate
keywords: Open tubular capillary electrochromatography; Porogen; Porous inner surface; Proteins