title: CD14+CD33+ myeloid cell-CCL11-eosinophil signature in ulcerative colitis.
abstract: This study tested the hypothesis that eotaxins (CCL11, CCL24, and CCL26) and IL-5 contribute to eosinophil recruitment to the intestine in UC and that intestinal macrophages are important producers of CCL11 in this disease. Peripheral blood and rectal biopsy samples were obtained from patients with active (n=18) and quiescent UC (n=9), and control patients (n=7). Eosinophil and macrophage levels and activation were analyzed by flow cytometry. Rectal mRNA levels of CCL11, CCL24, CCL26, and IL-5 were determined by qRT-PCR. The cellular source of CCL11 was visualized by immunofluorescence analyses. Eosinophil numbers were elevated in the blood and rectum of active and quiescent UC patients compared with controls. Levels of activated eosinophils (CD66b(high)) correlated with disease severity. Rectal CCL11, CCL24, and CCL26 mRNA levels were increased in active UC, whereas only CCL11 was elevated in quiescent UC. Levels of CCL11, but not CCL24 and CCL26, positively correlated with eosinophil numbers. Numbers of CD14(+)CD33(+) cells correlated with CCL11 and eosinophil levels. Immunofluorescence analyses revealed the presence of CD14(+)CCL11(+) mononuclear cells in colonic biopsies in UC. These results support the hypothesis that CCL11 contributes to eosinophil recruitment in UC and that intestinal myeloid cells are a source of CCL11. Interestingly, rectal levels of CCL24, CCL26, and IL-5 only increase during active UC, coinciding with further elevation of eosinophil numbers and with the activation of rectal eosinophils. In conclusion, there is a link among CD14(+)CD33(+) myeloid cells, CCL11, and eosinophils in adult UC.
authors.affiliation: 2.Cincinnati Children's Hospital Medical Center, 3333 Burnet Ave, ML7028, Cincinnati, OH, 45229; E-mail, simon.hogan@cchmc.org; and Gastroenterology Research Group, Department of Medical Sciences, University Hospital, Uppsala 751 85 Sweden; E-mail, maria.lampinen@medsci.uu.se.
authors.forename: Maria
authors.initials: M
authors.lastname: Lampinen
authors.forename: Amanda
authors.initials: A
authors.lastname: Waddell
authors.forename: Richard
authors.initials: R
authors.lastname: Ahrens
authors.forename: Marie
authors.initials: M
authors.lastname: Carlson
authors.forename: Simon P
authors.initials: SP
authors.lastname: Hogan
mesh_terms: D000328:Adult; D000368:Aged; D054413:Chemokine CCL11 / Q000032:analysis / Q000235:genetics / Q000502:physiology*; D003093:Colitis, Ulcerative / Q000276:immunology*; D004804:Eosinophils / Q000276:immunology*; D005260:Female; D006801:Humans; D018950:Lipopolysaccharide Receptors / Q000032:analysis*; D008297:Male; D008875:Middle Aged; D022423:Myeloid Cells / Q000276:immunology*; D012333:RNA, Messenger / Q000032:analysis; D012007:Rectum / Q000276:immunology; D063268:Sialic Acid Binding Ig-like Lectin 3 / Q000032:analysis*
chemical_list: C515456:CCL11 protein, human; D054413:Chemokine CCL11; D018950:Lipopolysaccharide Receptors; D012333:RNA, Messenger; D063268:Sialic Acid Binding Ig-like Lectin 3
keywords: chemokine; eotaxins; inflammatory bowel disease
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