title: Regulator of G protein signaling 19 suppresses Ras-induced neoplastic transformation and tumorigenesis.
abstract: Regulator of G protein signaling 19 (RGS19) has recently been shown to inhibit Ras activation by upregulating the tumor metastasis suppressor Nm23. Here, we have examined the effect of RGS19 on Ras-induced oncogenesis. Coexpression of RGS19, but not RGS20, in NIH3T3 cells effectively suppressed neoplastic transformation and tumorigenesis induced by the oncogenic Ras(GV) mutant. In non-small cell lung carcinoma H1299 cells that harbor Ras mutations, shRNA-mediated knockdown of RGS19 facilitated tumorigenesis with the early appearance of large tumors in nude mice assays. Collectively, these results suggest that expression of RGS19 can suppress the oncogenic actions of Ras.
authors.affiliation: Division of Life Science and the Biotechnology Research Institute, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong.
authors.forename: Yingchun
authors.initials: Y
authors.lastname: Wang
authors.forename: Yao
authors.initials: Y
authors.lastname: Tong
authors.forename: Prudence H
authors.initials: PH
authors.lastname: Tso
authors.forename: Yung H
authors.initials: YH
authors.lastname: Wong
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002471:Cell Transformation, Neoplastic / Q000235:genetics / Q000378:metabolism*; D005260:Female; D015870:Gene Expression; D055785:Gene Knockdown Techniques; D006367:HeLa Cells; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D041681:NIH 3T3 Cells; D054778:NM23 Nucleoside Diphosphate Kinases / Q000235:genetics / Q000378:metabolism; D016283:Proto-Oncogene Proteins p21(ras) / Q000235:genetics / Q000378:metabolism*; D020710:RGS Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D054778:NM23 Nucleoside Diphosphate Kinases; D020710:RGS Proteins; C097187:regulator of G-protein signalling 19; D016283:Proto-Oncogene Proteins p21(ras)
keywords: ERK; GAIP; Nm23; RGS19; Ras