title: Metabolism of four α-glycosidic linkage-containing oligosaccharides by Bifidobacterium breve UCC2003.
abstract: Members of the genus Bifidobacterium are common inhabitants of the gastrointestinal tracts of humans and other mammals, where they ferment many diet-derived carbohydrates that cannot be digested by their hosts. To extend our understanding of bifidobacterial carbohydrate utilization, we investigated the molecular mechanisms by which 11 strains of Bifidobacterium breve metabolize four distinct α-glucose- and/or α-galactose-containing oligosaccharides, namely, raffinose, stachyose, melibiose, and melezitose. Here we demonstrate that all B. breve strains examined possess the ability to utilize raffinose, stachyose, and melibiose. However, the ability to metabolize melezitose was not common to all B. breve strains tested. Transcriptomic and functional genomic approaches identified a gene cluster dedicated to the metabolism of α-galactose-containing carbohydrates, while an adjacent gene cluster, dedicated to the metabolism of α-glucose-containing melezitose, was identified in strains that are able to use this carbohydrate.
authors.affiliation: Department of Microbiology.
authors.forename: Kerry Joan
authors.initials: KJ
authors.lastname: O'Connell
authors.forename: Mary
authors.initials: M
authors.lastname: O'Connell Motherway
authors.forename: John
authors.initials: J
authors.lastname: O'Callaghan
authors.forename: Gerald F
authors.initials: GF
authors.lastname: Fitzgerald
authors.forename: R Paul
authors.initials: RP
authors.lastname: Ross
authors.forename: Marco
authors.initials: M
authors.lastname: Ventura
authors.forename: Catherine
authors.initials: C
authors.lastname: Stanton
authors.forename: Douwe
authors.initials: D
authors.lastname: van Sinderen
mesh_terms: D001644:Bifidobacterium / Q000302:isolation & purification / Q000378:metabolism*; D020869:Gene Expression Profiling; D006801:Humans; D008553:Melibiose / Q000378:metabolism*; D053858:Metabolic Networks and Pathways / Q000235:genetics; D005810:Multigene Family; D009844:Oligosaccharides / Q000378:metabolism*; D011887:Raffinose / Q000378:metabolism*
chemical_list: D009844:Oligosaccharides; C005695:stachyose; D008553:Melibiose; D011887:Raffinose
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