title: Immune responses in mice vaccinated with a suicidal DNA vaccine expressing the hemagglutinin glycoprotein from the peste des petits ruminants virus.
abstract: Peste des petits ruminants (PPR), an acute and highly contagious disease, affects sheep, goats, and some small ruminants. The hemagglutinin (H) glycoprotein of the PPR virus (PPRV) is considered important for inducing protective immune responses. In this study, a suicidal DNA vaccine based on the Semliki Forest virus (SFV) replicon was constructed and tested for its ability to induce immunogenicity in a mouse model. For this, the H gene of PPRV was cloned and inserted into pSCA1, an SFV replicon vector. The resultant plasmid named pSCA1-H was then transfected into BHK-21 cells following which the antigenicity of the expressed protein was confirmed by Western blotting and immunofluorescence. The pSCA1-H plasmid was then injected intramuscularly into BALB/c mice thrice at 2-week intervals. To evaluate the immunogenicity of pSCA1-H, specific antibodies and neutralizing antibodies against PPRV-H were measured using an indirect enzyme-linked immunosorbent assay and a microneutralization test, respectively. Cell-mediated immune responses were also examined using a lymphocyte proliferation assay. The results showed that pSCA1-H could express the H protein in BHK-21 cells. Specific antibodies, neutralizing antibodies, and lymphocyte proliferation responses were all induced in mice. Thus, this suicidal DNA vaccine could be a promising new approach for vaccine development against PPR.
authors.affiliation: State Key Laboratory of Animal Nutrition, Beijing Institute of Animal Science and Veterinary Medicine, Chinese Academy of Agricultural Sciences, Beijing 100193, China; College of Animal Science and Technology, Anhui Agricultural University, 130 Changjiang West Road, Hefei 230036, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Wang
authors.forename: Guangqing
authors.initials: G
authors.lastname: Liu
authors.forename: Lijun
authors.initials: L
authors.lastname: Shi
authors.forename: Wenchao
authors.initials: W
authors.lastname: Li
authors.forename: Chuanfeng
authors.initials: C
authors.lastname: Li
authors.forename: Zongyan
authors.initials: Z
authors.lastname: Chen
authors.forename: Hongyan
authors.initials: H
authors.lastname: Jin
authors.forename: Binrui
authors.initials: B
authors.lastname: Xu
authors.forename: Gang
authors.initials: G
authors.lastname: Li
mesh_terms: D000818:Animals; D057134:Antibodies, Neutralizing / Q000097:blood; D000914:Antibodies, Viral / Q000097:blood; D049109:Cell Proliferation; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005822:Genetic Vectors; D006388:Hemagglutinins / Q000235:genetics / Q000276:immunology*; D007273:Injections, Intramuscular; D008214:Lymphocytes / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D009500:Neutralization Tests; D029021:Peste-des-Petits-Ruminants / Q000517:prevention & control; D018107:Peste-des-petits-ruminants virus / Q000235:genetics / Q000276:immunology*; D012672:Semliki forest virus / Q000235:genetics; D014611:Vaccination / Q000379:methods; D019444:Vaccines, DNA / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D014765:Viral Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*
chemical_list: D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D006388:Hemagglutinins; D019444:Vaccines, DNA; D014614:Vaccines, Synthetic; D014765:Viral Vaccines
keywords: 3,3′-Diaminobenzidine tetrahydrochloride; 50% tissue culture infectious dose; African green monkey kidney cells; BHK-21; CPE; DAB; DIVA; DMEM; Dulbecco's Modified Eagle's Medium; H; HRP; Hemagglutinin glycoprotein; Immune response; Mouse; Nig 75/1; Nigeria 75/1; OIE; PPR; PPRV; Peste des petits ruminants virus; SFV; Semliki Forest virus; Suicidal DNA vaccine; TCID(50); TMB; VNA; Vero cell; World Organization for Animal Health; baby hamster kidney; cytopathic effect; differentiating infected from vaccinated animals; hemagglutinin; horseradish peroxidase; peste des petits ruminants; peste des petits ruminants virus; tetramethylbenzidine; virus-neutralizing antibody