title: Effects of Au/Fe and Fe nanoparticles on Serratia bacterial growth and production of biosurfactant.
abstract: The overall objective of this study was to compare the effects of Au/Fe and Fe nanoparticles on the growth and performance of Serratia Jl0300. The nanoparticle effect was quantified not only by the bacterial growth on agar plate after 1 hour interaction with the nanoparticles, but also by its production of a biosurfactant from used vegetable oil. The nanoparticles were prepared using the foam method. The concentrations of the nanoparticles used for the bacterial interaction study were varied from 1 mg/L to 1 g/L. The test results showed that the effect of nanoparticles on the bacterial growth and biosurfactant production varied with nanoparticle type, concentrations, and interaction time with the bacteria. Au/Fe nanoparticles didn't show toxicity to Serratia after short time (1 h) exposure, while during 8 days fermentation Au/Fe nanoparticles inhibited the growth of Serratia as well as the biosurfactant production when the concentration of the nanoparticles was higher than 10mg/L. Fe nanoparticles showed inhibition effects to bacterial growth both after short time and long time interaction with Serratia, as well as to biosurfactant production when its concentration was higher than 100 mg/L. Based on the trends observed in this study, analytical models have been developed to predict the bacterial growth and biosurfactant production with varying concentrations of nanoparticles.
authors.affiliation: Department of Civil and Environmental Engineering, University of Houston, Houston, TX 77204-4003, USA.
authors.forename: Jia
authors.initials: J
authors.lastname: Liu
authors.forename: Cumaraswamy
authors.initials: C
authors.lastname: Vipulanandan
mesh_terms: D001672:Biocompatible Materials / Q000378:metabolism*; D003198:Computer Simulation; D006046:Gold / Q000494:pharmacology*; D007501:Iron / Q000494:pharmacology*; D007700:Kinetics; D008954:Models, Biological; D053758:Nanoparticles / Q000648:ultrastructure; D012705:Serratia / Q000187:drug effects / Q000254:growth & development* / Q000648:ultrastructure; D013052:Spectrometry, X-Ray Emission; D013501:Surface-Active Agents / Q000378:metabolism*
chemical_list: D001672:Biocompatible Materials; D013501:Surface-Active Agents; D006046:Gold; D007501:Iron
keywords: Au/Fe nanoparticle; Biosurfactant; Fe nanoparticle; Interaction; Serratia