title: The peripheral blood compartment in patients with Graves' disease: activated T lymphocytes and increased transitional and pre-naive mature B lymphocytes.
abstract: Graves' disease (GD) is an autoimmune disease that involves aberrant B and T lymphocyte responses. Detailed knowledge about lymphocyte subpopulation composition will therefore enhance our understanding of the pathogenesis of GD and might support the development of new immunomodulatory treatment approaches. The aim of this study was to gain detailed insight into the composition of the peripheral blood lymphocyte compartment in GD before and during anti-thyroid drug therapy. Major B and T lymphocyte subpopulations were investigated by flow cytometry in peripheral blood from newly diagnosed GD patients (n = 5), GD patients treated with anti-thyroid drugs (n = 4), patients with recurrent GD (n = 7) and healthy controls (HC; n = 10). In GD patients, numbers of activated T lymphocytes [human leucocyte antigen D-related (HLA-DR)⁺ and CD25⁺] were increased. The B lymphocyte compartment in GD was characterized by significantly higher numbers of transitional (CD38(high) CD27⁻, P < 0.03) and pre-naive mature (CD38(low) CD27⁻ IgD⁺ CD5⁺, P < 0.04) B lymphocytes, while memory populations were slightly decreased. The increased numbers of CD5⁺, transitional and pre-naive mature B lymphocytes correlated positively with fT4 plasma levels. GD is associated with increased numbers of activated T lymphocytes and transitional and pre-naive mature CD5⁺ B lymphocytes within the peripheral blood. The increase in CD5⁺ B lymphocytes was due mainly to an increase in transitional and pre-naive mature B lymphocytes. Increased fT4 plasma levels might be associated with this increase in transitional and pre-naive mature CD5⁺ B lymphocytes.
authors.affiliation: Department of Immunology, Erasmus MC, University Medical Center, Rotterdam, the Netherlands.
authors.forename: K
authors.initials: K
authors.lastname: Van der Weerd
authors.forename: P M
authors.initials: PM
authors.lastname: Van Hagen
authors.forename: B
authors.initials: B
authors.lastname: Schrijver
authors.forename: D J
authors.initials: DJ
authors.lastname: Kwekkeboom
authors.forename: W W
authors.initials: WW
authors.lastname: De Herder
authors.forename: M R J
authors.initials: MR
authors.lastname: Ten Broek
authors.forename: P T E
authors.initials: PT
authors.lastname: Postema
authors.forename: J J M
authors.initials: JJ
authors.lastname: Van Dongen
authors.forename: F J T
authors.initials: FJ
authors.lastname: Staal
authors.forename: W A
authors.initials: WA
authors.lastname: Dik
mesh_terms: D000328:Adult; D015703:Antigens, CD / Q000378:metabolism; D001775:Blood Circulation / Q000276:immunology*; D002454:Cell Differentiation; D049109:Cell Proliferation; D005260:Female; D006111:Graves Disease / Q000276:immunology*; D006684:HLA-DR Antigens / Q000378:metabolism; D006801:Humans; D007156:Immunologic Memory; D016130:Immunophenotyping; D008213:Lymphocyte Activation; D016131:Lymphocyte Subsets / Q000276:immunology*; D054503:Lymphoid Progenitor Cells / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D013601:T-Lymphocytes / Q000276:immunology*; D055815:Young Adult
chemical_list: D015703:Antigens, CD; D006684:HLA-DR Antigens
keywords: B lymphocytes; Graves' disease; T lymphocytes
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