title: γδ T cells exhibit multifunctional and protective memory in intestinal tissues.
abstract: The study of T cell memory and the target of vaccine design have focused on memory subsumed by T cells bearing the αβ T cell receptor. Alternatively, γδ T cells are thought to provide rapid immunity, particularly at mucosal borders. Here, we have shown that a distinct subset of mucosal γδ T cells mounts an immune response to oral Listeria monocytogenes (Lm) infection and leads to the development of multifunctional memory T cells capable of simultaneously producing interferon-γ and interleukin-17A in the murine intestinal mucosa. Challenge infection with oral Lm, but not oral Salmonella or intravenous Lm, induced rapid expansion of memory γδ T cells, suggesting contextual specificity to the priming pathogen. Importantly, memory γδ T cells were able to provide enhanced protection against infection. These findings illustrate that γδ T cells play a role with hallmarks of adaptive immunity in the intestinal mucosa.
authors.affiliation: Center for Integrated Immunology and Vaccine Research, Department of Immunology, University of Connecticut Health Center, Farmington, CT 06030, USA.
authors.forename: Brian S
authors.initials: BS
authors.lastname: Sheridan
authors.forename: Pablo A
authors.initials: PA
authors.lastname: Romagnoli
authors.forename: Quynh-Mai
authors.initials: QM
authors.lastname: Pham
authors.forename: Han-Hsuan
authors.initials: HH
authors.lastname: Fu
authors.forename: Francis
authors.initials: F
authors.lastname: Alonzo
authors.forename: Wolf-Dieter
authors.initials: WD
authors.lastname: Schubert
authors.forename: Nancy E
authors.initials: NE
authors.lastname: Freitag
authors.forename: Leo
authors.initials: L
authors.lastname: Lefrançois
mesh_terms: D056704:Adaptive Immunity / Q000276:immunology; D000595:Amino Acid Sequence; D000818:Animals; D002478:Cells, Cultured; D005260:Female; D005434:Flow Cytometry; D054884:Host-Pathogen Interactions / Q000276:immunology; D018960:Hyaluronan Receptors / Q000276:immunology / Q000378:metabolism; D007156:Immunologic Memory / Q000276:immunology*; D007371:Interferon-gamma / Q000276:immunology / Q000378:metabolism; D020381:Interleukin-17 / Q000276:immunology / Q000378:metabolism; D007413:Intestinal Mucosa / Q000276:immunology / Q000378:metabolism; D007422:Intestines / Q000276:immunology*; D008089:Listeria monocytogenes / Q000276:immunology / Q000502:physiology; D008088:Listeriosis / Q000276:immunology / Q000378:metabolism; D051379:Mice; D020297:Mice, Congenic; D008807:Mice, Inbred BALB C; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D016176:T-Lymphocyte Subsets / Q000276:immunology / Q000378:metabolism; D013601:T-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7 / Q000276:immunology / Q000378:metabolism
chemical_list: D018960:Hyaluronan Receptors; D020381:Interleukin-17; D016692:Receptors, Antigen, T-Cell, gamma-delta; D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7; D007371:Interferon-gamma
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