title: Mechanical and biocompatible characterization of a cross-linked collagen-hyaluronic acid wound dressing.
abstract: Collagen scaffolds have been widely employed as a dermal equivalent to induce fibroblast infiltrations and dermal regeneration in the treatment of chronic wounds and diabetic foot ulcers. Cross-linking methods have been developed to address the disadvantages of the rapid degradation associated with collagen-based scaffolds. To eliminate the potential drawbacks associated with glutaraldehyde cross-linking, methods using a water soluble carbodiimide have been developed. In the present study, the glycosaminoglycan (GAG) hyaluronic acid (HA), was covalently attached to an equine tendon derived collagen scaffold using 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide (EDC) to create ntSPONGE The HA was shown to be homogeneously distributed throughout the collagen matrix. In vitro analyses of the scaffold indicated that the cross-linking enhanced the biological stability by decreasing the enzymatic degradation and increasing the thermal denaturation temperature. The material was shown to support the attachment and proliferation of mouse L929 fibroblast cells. In addition, the cross-linking decreased the resorption rate of the collagen as measured in an intramuscular implant model in rabbits. The material was also shown to be biocompatible in a variety of in vitro and in vivo assays. These results indicate that this cross-linked collagen-HA scaffold, ntSPONGE has the potential for use in chronic wound healing.
authors.affiliation: Research and Development Department; Nanotherapeutics, Inc.; Alachua, FL USA.
authors.forename: James F
authors.initials: JF
authors.lastname: Kirk
authors.affiliation: Research and Development Department; Nanotherapeutics, Inc.; Alachua, FL USA.
authors.forename: Gregg
authors.initials: G
authors.lastname: Ritter
authors.affiliation: Research and Development Department; Nanotherapeutics, Inc.; Alachua, FL USA.
authors.forename: Isaac
authors.initials: I
authors.lastname: Finger
authors.affiliation: Research and Development Department; Nanotherapeutics, Inc.; Alachua, FL USA.
authors.forename: Dhyana
authors.initials: D
authors.lastname: Sankar
authors.affiliation: Research and Development Department; Nanotherapeutics, Inc.; Alachua, FL USA.
authors.forename: Joseph D
authors.initials: JD
authors.lastname: Reddy
authors.affiliation: Research and Development Department; Nanotherapeutics, Inc.; Alachua, FL USA.
authors.forename: James D
authors.initials: JD
authors.lastname: Talton
authors.affiliation: MLM Biologics; Gainesville, FL USA.
authors.forename: Chandra
authors.initials: C
authors.lastname: Nataraj
authors.affiliation: Sanford-Burnham Medical Research Institute; La Jolla, CA USA.
authors.forename: Sonoko
authors.initials: S
authors.lastname: Narisawa
authors.affiliation: Sanford-Burnham Medical Research Institute; La Jolla, CA USA.
authors.forename: José Luis
authors.initials: JL
authors.lastname: Millán
authors.affiliation: Research and Development Department; Nanotherapeutics, Inc.; Alachua, FL USA.
authors.forename: Ronald R
authors.initials: RR
authors.lastname: Cobb
mesh_terms: D000818:Animals; D047070:Bandages, Hydrocolloid; D001672:Biocompatible Materials / Q000009:adverse effects / Q000138:chemical synthesis; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D003094:Collagen / Q000737:chemistry* / Q000633:toxicity*; D003432:Cross-Linking Reagents / Q000009:adverse effects / Q000737:chemistry; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects* / Q000502:physiology*; D006820:Hyaluronic Acid / Q000009:adverse effects* / Q000737:chemistry*; D008422:Materials Testing; D051379:Mice; D013314:Stress, Mechanical; D013718:Tensile Strength
chemical_list: D001672:Biocompatible Materials; D003432:Cross-Linking Reagents; D006820:Hyaluronic Acid; D003094:Collagen
keywords: collagen sponge; cross-linking; histology; hyaluronic acid; wound repair
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