title: Analysis of primitive erythroid cell proliferation and enucleation using a cyan fluorescent reporter in transgenic mice.
abstract: Primitive erythropoiesis is a vital process for mammalian embryonic development. Here we report the generation and characterization of a new transgenic mouse line that expresses a histone H2B-CFP fusion protein in the nuclei of primitive erythroid cells. We demonstrate the potential of this ε-globin-histone H2B-CFP line for multicolor imaging and flow cytometry analysis. The ε-globin-H2B-CFP line was used to analyze the cell cycle distribution and proliferation of CFP-expressing primitive erythroblasts from E8.5-E13.5. We also evaluated phagocytosis of extruded CFP-positive nuclei by macrophages in fetal liver and placenta. The ε-globin-H2B-CFP transgenic mouse line adds to the available tools for studying the development of the primitive erythroid lineage.
authors.affiliation: Department of Medicine, The Icahn School of Medicine at Mount Sinai, New York, New York; The Tisch Cancer Institute, The Icahn School of Medicine at Mount Sinai, New York, New York.
authors.forename: Andrei M
authors.initials: AM
authors.lastname: Vacaru
authors.forename: Joan
authors.initials: J
authors.lastname: Isern
authors.forename: Stuart T
authors.initials: ST
authors.lastname: Fraser
authors.forename: Margaret H
authors.initials: MH
authors.lastname: Baron
mesh_terms: D000818:Animals; D019070:Cell Lineage; D002467:Cell Nucleus / Q000502:physiology; D049109:Cell Proliferation; D004622:Embryo, Mammalian; D004900:Erythroblasts / Q000166:cytology / Q000502:physiology*; D004920:Erythropoiesis / Q000235:genetics; D017930:Genes, Reporter; D005838:Genotype; D049452:Green Fluorescent Proteins / Q000378:metabolism*; D006657:Histones / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008822:Mice, Transgenic; D010587:Phagocytosis; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D055547:epsilon-Globins / Q000235:genetics / Q000378:metabolism
chemical_list: C485182:Cyan Fluorescent Protein; D006657:Histones; D011993:Recombinant Fusion Proteins; D055547:epsilon-Globins; D049452:Green Fluorescent Proteins
keywords: cyan fluorescent protein; fluorescent reporter; histone fusion; imaging; primitive erythropoiesis; transgenic mice
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