title: Determination of κFLC and κ Index in cerebrospinal fluid: a valid alternative to assess intrathecal immunoglobulin synthesis.
abstract: Intrathecal immunoglobulin synthesis is observed in several disorders of the central nervous system, but its detection by current laboratory tests is relatively insensitive and operator depending. We assessed the diagnostic accuracy of a nephelometric assay for k free light chain determination in cerebrospinal fluid and serum. The patients were grouped according to clinical and laboratory criteria. ROC curves for all methods were performed to find the best cut-off value. kFLC Index seems to be more accurate than other parameters. Our data indicate that nephelometric assay for kFLCs in CSF reliably detect intrathecal immunoglobulin synthesis and discriminate multiple sclerosis patients.
authors.affiliation: Department of Experimental Medicine and Surgery, "Tor Vergata" University Hospital, Rome, Italy.
authors.forename: Fabio
authors.initials: F
authors.lastname: Duranti
authors.forename: Massimo
authors.initials: M
authors.lastname: Pieri
authors.forename: Diego
authors.initials: D
authors.lastname: Centonze
authors.forename: Fabio
authors.initials: F
authors.lastname: Buttari
authors.forename: Sergio
authors.initials: S
authors.lastname: Bernardini
authors.forename: Mariarita
authors.initials: M
authors.lastname: Dessi
mesh_terms: D000328:Adult; D000368:Aged; D015415:Biomarkers / Q000097:blood / Q000134:cerebrospinal fluid; D005260:Female; D006801:Humans; D007145:Immunoglobulin kappa-Chains / Q000096:biosynthesis* / Q000097:blood / Q000134:cerebrospinal fluid*; D007278:Injections, Spinal; D008297:Male; D008875:Middle Aged; D009103:Multiple Sclerosis / Q000134:cerebrospinal fluid* / Q000175:diagnosis / Q000276:immunology*; D043025:Oligoclonal Bands / Q000096:biosynthesis / Q000097:blood / Q000134:cerebrospinal fluid; D015203:Reproducibility of Results; D012720:Severity of Illness Index; D055815:Young Adult
chemical_list: D015415:Biomarkers; D007145:Immunoglobulin kappa-Chains; D043025:Oligoclonal Bands
keywords: CNS; CSF; CSF/serum ratio of albumin; FLC; Ig; MS; Multiple Sclerosis; Multiple sclerosis; Nephelometry; OCBs; Q(alb); central nervous system; cerebrospinal fluid; free light chain; immunoglobulin; kFLC Index; kFLCs; oligoclonal immunoglobulin bands