title: Chemical mutagenesis of Gluconobacter frateurii to construct methanol-resistant mutants showing glyceric acid production from methanol-containing glycerol.
abstract: To produce glyceric acid (GA) from methanol-containing glycerol, resistance to methanol of Gluconobacter frateurii NBRC103465 was improved by chemical mutagenesis using N-methyl-N'-nitro-N-nitrosoguanidine. The obtained mutant Gf398 produced 6.3 g/L GA in 5% (v/v) methanol-containing 17% (w/v) glycerol medium, in which the wild-type strain neither grew nor produced GA.
authors.affiliation: Research Institute for Innovation in Sustainable Chemistry, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central 5-2, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8565, Japan.
authors.forename: Shun
authors.initials: S
authors.lastname: Sato
authors.affiliation: Research Institute for Innovation in Sustainable Chemistry, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central 5-2, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8565, Japan.
authors.forename: Dai
authors.initials: D
authors.lastname: Kitamoto
authors.affiliation: Research Institute for Innovation in Sustainable Chemistry, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central 5-2, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8565, Japan. Electronic address: hiroshi.habe@aist.go.jp.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Habe
mesh_terms: D020576:Gluconobacter / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*; D005988:Glyceric Acids / Q000378:metabolism*; D005990:Glycerol / Q000378:metabolism*; D000432:Methanol / Q000378:metabolism* / Q000494:pharmacology; D008769:Methylnitronitrosoguanidine / Q000494:pharmacology; D016296:Mutagenesis / Q000187:drug effects
chemical_list: D005988:Glyceric Acids; D008769:Methylnitronitrosoguanidine; C042971:glyceric acid; D005990:Glycerol; D000432:Methanol
keywords: Acetic acid bacteria; Gluconobacter frateurii; Glyceric acid; Glycerol use; Methanol; Raw glycerol