title: Layer-by-layer assembled gold nanoparticles with a tunable payload of a nitric oxide photocage.
abstract: We report herein the design, preparation and characterisation of a new, water soluble, nanoplatform for the light-triggered release of nitric oxide (NO). This nanoconstruct has been achieved by exploiting a layer-by-layer approach to assembly a polyelectrolyte modified with a NO photochemical precursor, around citrate stabilized gold colloidal template. Combined spectroscopic and transmission electron microscopy analysis show that the layered hybrid nanoparticles remain stable under physiological conditions without any significant clustering along the different coating processes. The photochemical properties of the NO photocage are well preserved upon its covalent grafting in the polymeric skeleton, before and after the of the metal colloids. The direct and in real time monitoring of NO using an ultrasensitive electrode unambiguously shows the light-stimulated NO release from modified gold nanoparticles and demonstrates that the approach used permits the accurate regulation of the NO reservoir on the nanoparticles surface.
authors.affiliation: Departamento de Quimica Fisica, Universidade de Vigo, 36310 Vigo, Spain.
authors.forename: Patricia
authors.initials: P
authors.lastname: Taladriz-Blanco
authors.forename: Jorge
authors.initials: J
authors.lastname: Pérez-Juste
authors.forename: Noufal
authors.initials: N
authors.lastname: Kandoth
authors.forename: Pablo
authors.initials: P
authors.lastname: Hervés
authors.forename: Salvatore
authors.initials: S
authors.lastname: Sortino
mesh_terms: D006046:Gold / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D046529:Microscopy, Electron, Transmission; D009569:Nitric Oxide / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet
chemical_list: D009569:Nitric Oxide; D006046:Gold
keywords: Gold nanoparticles; Layer-by-layer; Light; Nitric oxide; Release