title: Neuroprotective effect of olive oil in the hippocampus CA1 neurons following ischemia: Reperfusion in mice.
abstract: BACKGROUND
  Transient global ischemia induces selective, delayed neuronal death of pyramidal neurons in the hippocampal CA1. Oxidative Stress is considered to be involved in a number of human diseases including ischemia. Preliminary studies confirmed reduction of cell death in brain following treatment with antioxidants.
  
  
  OBJECTIVE
  According to this finding, we study the relationship between consumption of olive oil on cell death and memory disorder in brain ischemia. We studied the protective effect of olive oil against ischemia-reperfusion.
  
  
  METHODS
  EXPERIMENTAL DESIGN INCLUDES THREE GROUPS: Intact (n = 8), ischemic control (n = 8) and treatment groups with olive oil (n = 8). The mice treated with olive oil as pre-treatment for a week. Then, ischemia induced by common carotid artery ligation and following the reduction of inflammation [a week after ischemia], the mice post-treated with olive oil. Nissl staining applied for counting necrotic cells in hippocampus CA1. Tunnel kit was used to quantify apoptotic cell death while to short term memory scale, we apply y-maze and shuttle box tests and for detection the rate of apoptotic and treated cell, we used western blotting test for bax and bcl2 proteins.
  
  
  RESULTS
  High rate of apoptosis was seen in ischemic group that significantly associated with short-term memory loss. Cell death was significantly lower when mice treated with olive oil. The memory test results were adjusted with cell death results and bax and bcl2 expression in all groups' comparison. Ischemia for 15 min induced cell death in hippocampus with more potent effect on CA1.
  
  
  CONCLUSIONS
  Olive oil intake significantly reduced cell death and decreased memory loss.
authors.affiliation: Department of Anatomy, Esfahan University of Medical Sciences, Esfahan, Iran.
authors.forename: M
authors.initials: M
authors.lastname: Zamani
authors.forename: J
authors.initials: J
authors.lastname: Hassanshahi
authors.forename: M
authors.initials: M
authors.lastname: Soleimani
authors.forename: F
authors.initials: F
authors.lastname: Zamani
keywords: Antioxidant; hippocampus CA1; ischemia-reperfusion; neuroprotective effect; olive oil
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