title: Expression of recombinant antigenic proteins from Angiostrongylus cantonensis: a brief report.
abstract: Cerebral angiostrongyliasis is an acute inflammation caused by the infection of the nematode Angiostrongylus cantonensis that results in eosinophilic meningitis. The current immunological assay of choice is an immunoblot that detects antibodies to a 31 kDa protein present in crude extracts of the female worm. Recently we have identified diagnostic targets from excretion and secretion products and determined the composition of the 31 kDa antigen after 2-D gel electrophoresis and mass spectrometry. Here we cloned and expressed five proteins in prokaryotic and eukaryotic systems. Recombinant proteins were purified and analysed by Western blot assays and among them 14-3-3, Lec5 and ES7 were recognized by Angiostrongylus-specific serum, although the signal was weak.
authors.affiliation: Laboratório de Biologia Parasitária da Faculdade de Biociências e Laboratório de Parasitologia Molecular do Instituto de Pesquisas Biomédicas da Pontifícia Universidade do Rio Grande do Sul (PUCRS), Brazil. almorassutti@gmail.com
authors.forename: Alessandra L
authors.initials: AL
authors.lastname: Morassutti
authors.forename: Andrey
authors.initials: A
authors.lastname: Perelygin
authors.forename: Keith
authors.initials: K
authors.lastname: Levert
authors.forename: Seh-Ching
authors.initials: SC
authors.lastname: Lin
authors.forename: Yeuk-Mui
authors.initials: YM
authors.lastname: Lee
authors.forename: Alexandre J
authors.initials: AJ
authors.lastname: da Silva
authors.forename: Patricia P
authors.initials: PP
authors.lastname: Wilkins
authors.forename: Carlos
authors.initials: C
authors.lastname: Graeff-Teixeira
mesh_terms: D000595:Amino Acid Sequence; D017185:Angiostrongylus cantonensis / Q000276:immunology*; D000818:Animals; D000947:Antigens, Helminth / Q000737:chemistry / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D015153:Blotting, Western; D002846:Chromatography, Affinity; D011994:Recombinant Proteins / Q000737:chemistry / Q000276:immunology* / Q000302:isolation & purification; D017206:Strongylida Infections / Q000175:diagnosis*
chemical_list: C000588424:31-kDa antigen, Angiostrongylus cantonensis; D000947:Antigens, Helminth; D011994:Recombinant Proteins
keywords: 31 kDa antigen; Angiostrongylus; Eosinophilic meningitis; Recombinant protein
references.citation: Exp Parasitol. 2012 Jan;130(1):26-31
references.pmid: 22019415
references.citation: Anal Biochem. 1976 May 7;72:248-54
references.pmid: 942051
references.citation: Mem Inst Oswaldo Cruz. 2010 Nov;105(7):914-7
references.pmid: 21120363
references.citation: Southeast Asian J Trop Med Public Health. 2001 Jun;32(2):308-13
references.pmid: 11556581
references.citation: Exp Parasitol. 2008 Aug;119(4):516-23
references.pmid: 18316081
references.citation: Vector Borne Zoonotic Dis. 2012 Nov;12(11):961-8
references.pmid: 22925026
references.citation: Southeast Asian J Trop Med Public Health. 2000;31 Suppl 1:48-53
references.pmid: 11414459
references.citation: Biochemistry. 2008 Feb 12;47(6):1768-77
references.pmid: 18181650
references.citation: Parasitology. 2001 Dec;123(Pt 6):573-82
references.pmid: 11814044
references.citation: Int J Infect Dis. 2009 Jul;13(4):425-31
references.pmid: 19117782
references.citation: Exp Parasitol. 2009 Oct;123(2):162-7
references.pmid: 19559023
references.citation: Clin Microbiol Rev. 2009 Apr;22(2):322-48, Table of Contents
references.pmid: 19366917
references.citation: Biol Chem. 2006 Sep;387(9):1227-36
references.pmid: 16972791