title: Preparation and characterization of oligochitosan-tragacanth nanoparticles as a novel gene carrier.
abstract: The nanoparticles of oligochitosan-water soluble tragacanth (OCH-WST) as novel gene carriers have been prepared and their transfection efficiency has been investigated on Hela and HepG2 cell lines. Different OCH:WST weight ratios were prepared to obtain particles with low size distribution and high surface charge, and also in range of below 200 nm. Nanoparticles with 132.5 ± 6.77 nm size, polydispersity index 1.92 ± 0.061, surface charge 30.45 ± 1.84 and spherical morphology, have been chosen as gene carrier. Nanoparticle-DNA complexes (nanoplexes) showed better transfection efficiency in both Hela and HepG2 cells than chitosan polyplexes, with 1.26 × 10(6) versus 9.05 × 10(5) and 7.76 × 10(5) versus 2.16 × 10(5), respectively. Higher transfection efficiency of nanoplexes could be attributed to their weaker complexation. Decreasing of transfection in presence of galactose in HepG2 cells, indicated receptor mediated endocytosis of nanoplexes. These properties all together, make OCH-WST nanoparticles as potential gene carrier for active gene delivery into cells containing sugar receptors.
authors.affiliation: Department of Pharmaceutics, School of Pharmacy, Kermanshah University of Medical Sciences, Kermanshah 67346-67149, Iran. alifattahi@kums.ac.ir
authors.forename: Ali
authors.initials: A
authors.lastname: Fattahi
authors.forename: Komail
authors.initials: K
authors.lastname: Sadrjavadi
authors.forename: Mohammad Ali
authors.initials: MA
authors.lastname: Golozar
authors.forename: Jaleh
authors.initials: J
authors.lastname: Varshosaz
authors.forename: Mohammad-Hossein
authors.initials: MH
authors.lastname: Fathi
authors.forename: Hamid
authors.initials: H
authors.lastname: Mirmohammad-Sadeghi
mesh_terms: D002470:Cell Survival; D002686:Chitin / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry; D048271:Chitosan; D004587:Electrophoresis, Agar Gel; D018014:Gene Transfer Techniques; D006367:HeLa Cells; D056945:Hep G2 Cells; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008156:Luciferases / Q000378:metabolism; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D009844:Oligosaccharides; D010316:Particle Size; D012995:Solubility; D017550:Spectroscopy, Fourier Transform Infrared; D055672:Static Electricity; D014144:Tragacanth / Q000138:chemical synthesis* / Q000737:chemistry*; D014162:Transfection; D014867:Water / Q000737:chemistry
chemical_list: D009844:Oligosaccharides; C493484:oligochitosan; D014867:Water; D002686:Chitin; D014144:Tragacanth; D048271:Chitosan; D008156:Luciferases
keywords: Nanoplex; Non-viral gene carrier; Oligochitosan; Water soluble tragacanth