title: Intestinimonas butyriciproducens gen. nov., sp. nov., a butyrate-producing bacterium from the mouse intestine.
abstract: A Gram-positive, spore-forming, non-motile, strictly anaerobic rod-shaped bacterium was isolated from the caecal content of a TNF(deltaARE) mouse. The isolate, referred to as strain SRB-521-5-I(T), was originally cultured on a reduced agar medium containing yeast extract, rumen fluid and lactic acid as main energy and carbon sources. Phylogenetic analysis of partial 16S rRNA genes revealed that the species most closely related to strain SRB-521-5-I(T) were Flavonifractor plautii and Pseudoflavonifractor capillosus (<95 % sequence similarity; 1436 bp). In contrast to F. plautii and P. capillosus, strain SRB-521-5-I(T) contained a substantial amount of C18 : 0 dimethylacetal. Additional major fatty acids were C14 : 0 methyl ester, C16 : 0 dimethylacetal and C18 : 0 aldehyde. Strain SRB-521-5-I(T) differed in its enzyme profile from F. plautii and P. capillosus by being positive for dextrin, maltotriose, turanose, dl-lactic acid and d-lactic acid methyl ester but negative for d-fructose. In reduced Wilkins-Chalgren-Anaerobe broth, strain SRB-521-5-I(T) produced approximately 8 mM butyrate and 4 mM acetate. In contrast to F. plautii, the strain did not metabolize flavonoids. It showed intermediate resistance towards the antibiotics ciprofloxacin, colistin and tetracycline. Based on genotypic and phenotypic characteristics, we propose the name Intestinimonas butyriciproducens gen. nov., sp. nov. to accommodate strain SRB-521-5-I(T) ( = DSM 26588(T) = CCUG 63529(T)) as the type strain.
authors.affiliation: Department of Gastrointestinal Microbiology, German Institute of Human Nutrition Potsdam-Rehbrücke (DIfE), Nuthetal, Germany.|ZIEL-Research Center for Nutrition and Food Sciences, Junior Research Group Intestinal Microbiome, Biofunctionality Unit, Technische Universität München (TUM), Freising, Germany.
authors.forename: Karoline
authors.initials: K
authors.lastname: Kläring
authors.affiliation: Department of Gastrointestinal Microbiology, German Institute of Human Nutrition Potsdam-Rehbrücke (DIfE), Nuthetal, Germany.
authors.forename: Laura
authors.initials: L
authors.lastname: Hanske
authors.affiliation: Laboratory of Microbiology, Wageningen University, The Netherlands.
authors.forename: Nam
authors.initials: N
authors.lastname: Bui
authors.affiliation: NovaBiotics Ltd, Aberdeen, UK.
authors.forename: Cédric
authors.initials: C
authors.lastname: Charrier
authors.affiliation: Department of Gastrointestinal Microbiology, German Institute of Human Nutrition Potsdam-Rehbrücke (DIfE), Nuthetal, Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Blaut
authors.affiliation: Chair of Nutrition and Immunology, Technische Universität München, Freising, Germany.
authors.forename: Dirk
authors.initials: D
authors.lastname: Haller
authors.affiliation: Laboratory of Microbiology, Wageningen University, The Netherlands.
authors.forename: Caroline M
authors.initials: CM
authors.lastname: Plugge
authors.affiliation: Chair of Nutrition and Immunology, Technische Universität München, Freising, Germany.|ZIEL-Research Center for Nutrition and Food Sciences, Junior Research Group Intestinal Microbiome, Biofunctionality Unit, Technische Universität München (TUM), Freising, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Clavel
mesh_terms: D000818:Animals; D001421:Bacteria, Anaerobic / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002087:Butyrates / Q000378:metabolism*; D004269:DNA, Bacterial / Q000235:genetics; D005260:Female; D016937:Gram-Positive Endospore-Forming Rods / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D007422:Intestines / Q000382:microbiology*; D051379:Mice; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D002087:Butyrates; D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S