title: Unscheduled DNA synthesis (UDS) test with mammalian liver cells in vivo.
abstract: The unscheduled DNA synthesis test is a short-term genotoxicity assay that allows identification of substances that induce DNA repair in liver cells of treated animals. This endpoint can be assessed by determining the uptake of radiolabelled nucleotide after excision-repair in cells that are not undergoing scheduled (S-phase) DNA synthesis. Here we describe the protocol for performing this well-known primary DNA damage test following the OECD guideline No. 486, EC, and published international recommendations.
authors.affiliation: Genetic Toxicology Department, Institut Pasteur de Lille, Lille, France.
authors.forename: Fabrice
authors.initials: F
authors.lastname: Nesslany
mesh_terms: D000818:Animals; D001345:Autoradiography; D018929:Cell Culture Techniques; D002469:Cell Separation; D002470:Cell Survival; D004247:DNA / Q000096:biosynthesis* / Q000235:genetics*; D004249:DNA Damage; D004260:DNA Repair; D004261:DNA Replication; D022781:Hepatocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D007553:Isotope Labeling; D009152:Mutagenicity Tests / Q000379:methods*; D051381:Rats
chemical_list: D004247:DNA