title: Nanocarriers with ultrahigh chromophore loading for fluorescence bio-imaging and photodynamic therapy.
abstract: We describe the design of original nanocarriers that allows for ultrahigh chromophore loading while maintaining the photo-activity of each individual molecule. They consist in shells of charged biocompatible polymers grafted on gold nanospheres. The self-organization of extended polymer chains results from repulsive charges and steric interactions that are optimized by tuning the surface curvature of nanoparticles. This type of nano-scaffolds can be used as light-activated theranostic agents for fluorescence imaging and photodynamic therapy. We demonstrate that, labeled with a fluorescent photosensitizer, it can localize therapeutic molecules before triggering the cell death of B16-F10 melanoma with an efficiency that is similar to the efficiency of the polymer conjugate alone, and with the advantage of extremely high local loading of photosensitizers (object concentration in the picomolar range).
authors.affiliation: Laboratoire de Chimie UMR 5182, Ecole Normale Supérieure de Lyon, CNRS, université Lyon 1, 46, allée d'Italie, Lyon cedex 07 F-69364, France.
authors.forename: Julien R G
authors.initials: JR
authors.lastname: Navarro
authors.forename: Frédéric
authors.initials: F
authors.lastname: Lerouge
authors.forename: Cristina
authors.initials: C
authors.lastname: Cepraga
authors.forename: Guillaume
authors.initials: G
authors.lastname: Micouin
authors.forename: Arnaud
authors.initials: A
authors.lastname: Favier
authors.forename: Denis
authors.initials: D
authors.lastname: Chateau
authors.forename: Marie-Thérèse
authors.initials: MT
authors.lastname: Charreyre
authors.forename: Pierre-Henri
authors.initials: PH
authors.lastname: Lanoë
authors.forename: Cyrille
authors.initials: C
authors.lastname: Monnereau
authors.forename: Frédéric
authors.initials: F
authors.lastname: Chaput
authors.forename: Sophie
authors.initials: S
authors.lastname: Marotte
authors.forename: Yann
authors.initials: Y
authors.lastname: Leverrier
authors.forename: Jacqueline
authors.initials: J
authors.lastname: Marvel
authors.forename: Kenji
authors.initials: K
authors.lastname: Kamada
authors.forename: Chantal
authors.initials: C
authors.lastname: Andraud
authors.forename: Patrice L
authors.initials: PL
authors.lastname: Baldeck
authors.forename: Stephane
authors.initials: S
authors.lastname: Parola
mesh_terms: D045744:Cell Line, Tumor; D005453:Fluorescence; D006046:Gold / Q000737:chemistry*; D006801:Humans; D015394:Molecular Structure; D053758:Nanoparticles / Q000737:chemistry*; D061848:Optical Imaging / Q000379:methods*; D010778:Photochemotherapy / Q000379:methods*
chemical_list: D006046:Gold
keywords: Bio-imaging; Fluorescence; Gold; Nanocarriers; PDT