title: Use of 1H NMR STD, waterLOGSY, and Langmuir monolayer techniques for characterization of drug-zein protein complexes.
abstract: Zein is a protein based natural biopolymer containing a large amount of nonpolar amino acids, which has shown the ability to form aggregates and entrap solutes, such as drugs and amino acids to form stable protein-drug complexes. In this work, π-A isotherm, NMR, and Dynamic light scattering were used to detect the formation of protein aggregates and the affinity between zein and two different drugs: tetracycline and indomethacin. An effective interaction of zein and the two drugs was evidenced by means of liquid NMR reinforced by means of changes in the surface pressure by π-A isotherm. The effective interactions zein/drugs under air/water interface were evidenced as a change in the surface pressure of the π-A isotherm of zein in the presence of drug solutions. The presence of tetracycline in the subphase decreased the area occupied by the monolayer at the expanded region until pressures of 12 mN/m were the areas became similar, but indomethacin produces an increment of the area in both expanded and collapsed region. The feasible methodology employed, focused in the functionality of the protein-drug interaction, can be very promising in the drug delivery field.
authors.affiliation: Department of Pharmacy and Pharmaceutical Technology, School of Pharmacy, University of Santiago de Compostela, Santiago de Compostela, Spain; Department of Biological and Health Sciences, School of Pharmacy, University Federal of Amapá, Macapá, Brazil. Electronic address: Phabio_oliveira@yahoo.com.br.
authors.forename: F F O
authors.initials: FF
authors.lastname: Sousa
authors.forename: A
authors.initials: A
authors.lastname: Luzardo-Álvarez
authors.forename: J
authors.initials: J
authors.lastname: Blanco-Méndez
authors.forename: F J
authors.initials: FJ
authors.lastname: Otero-Espinar
authors.forename: M
authors.initials: M
authors.lastname: Martín-Pastor
authors.forename: I
authors.initials: I
authors.lastname: Sández Macho
mesh_terms: D002626:Chemistry, Pharmaceutical / Q000379:methods; D004337:Drug Carriers / Q000737:chemistry*; D016503:Drug Delivery Systems; D005240:Feasibility Studies; D007213:Indomethacin / Q000737:chemistry*; D008027:Light; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D012542:Scattering, Radiation; D013678:Technology, Pharmaceutical / Q000379:methods; D013752:Tetracycline / Q000737:chemistry*; D015028:Zein / Q000737:chemistry*
chemical_list: D004337:Drug Carriers; D015028:Zein; D013752:Tetracycline; D007213:Indomethacin
keywords: Biopolymers; Brewster angle microscopy; Drug–protein complexes; Indomethacin; Maize protein; NMR; Tetracycline; Zein; π–A isotherm