title: The tandem Mannich-electrophilic amination reaction: a versatile platform for fluorescent probing and labeling.
abstract: Tandem fluorogenic reaction: A new platform for the direct, selective and sensitive detection of formaldehyde and/or secondary aliphatic amines, based on a tandem Mannich-electrophilic amination reaction, is described. Biological applications of hydrophilic, water-soluble fluorescent 2,2-dialkyl-2,3-dihydro-[1,2,4]triazolo[4,3-a]pyridin-2-ium-8-carboxylates (Safarinium P probes) are exemplified by labeling of the amine-containing Ac-AKF-NH2 peptide and Bacillus subtilis spores in aqueous solution.
authors.affiliation: Department of Chemical Technology of Drugs, Medical University of Gdańsk, Al. Gen. J. Hallera 107, 80-416 Gdańsk, Poland. js@gumed.edu.pl
authors.forename: Jarosław
authors.initials: J
authors.lastname: Sączewski
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Hinc
authors.forename: Michał
authors.initials: M
authors.lastname: Obuchowski
authors.forename: Maria
authors.initials: M
authors.lastname: Gdaniec
mesh_terms: D000586:Amination; D005456:Fluorescent Dyes / Q000627:therapeutic use*; D008352:Mannich Bases / Q000737:chemistry*
chemical_list: D005456:Fluorescent Dyes; D008352:Mannich Bases
keywords: amination; dyes/pigments; fluorogenic reaction; mannich bases; tandem reaction