title: A study of the bactericidal, anti-biofouling, cytotoxic and antioxidant properties of actinobacterially synthesised silver nanoparticles.
abstract: Actinobacteria- mediated synthesis of silver nanoparticles (AgNPs) is a reliable, eco-friendly and important aspect of nanobiotechnology. In this study, aqueous silver ions, which were exposed to an actinobacterial biomass of Streptomyces naganishii (MA7), were reduced to form stable AgNPs under optimised conditions. The microbially synthesised AgNPs were characterised by UV-Vis spectroscopy, Fourier transform infrared (FT-IR) spectroscopy, X-ray diffraction (XRD), selected area electron diffraction (SAED), energy dispersive X-ray spectroscopy (EDX), scanning electron microscopy (SEM), atomic force microscopy (AFM) and high- resolution transmission electron microscopy (HR-TEM). The size (5-50 nm) and shape (spherical) of the AgNPs were determined. The biosynthesised AgNPs exhibited good bactericidal, anti-biofouling, antioxidant and cytotoxic effects with regards to the HeLa cell line. A single protein band with a molecular weight of 44 kDa was obtained after partial purification of the culture filtrate via polyacrylamide gel electrophoresis. The potent actinobacterial strain was identified by its molecular (16s rRNA sequencing), phenotypic and cultural characteristics. The current study demonstrated the potential use of the extremophilic actinobacterial strain of S. naganishii (MA7) as a novel source for AgNPs synthesis with improved biomedical applications.
authors.affiliation: Actinobacterial Research Laboratory, Department of Microbiology, Periyar University, Periyar Palkalai Nagar, Salem 636 011, Tamil Nadu, India.
authors.forename: Thangavel
authors.initials: T
authors.lastname: Shanmugasundaram
authors.affiliation: Department of Bacteriology, National Institute for Research in Tuberculosis, Chennai, 600 031, Tamil Nadu, India.
authors.forename: Manikkam
authors.initials: M
authors.lastname: Radhakrishnan
authors.affiliation: Actinobacterial Research Laboratory, Department of Microbiology, Periyar University, Periyar Palkalai Nagar, Salem 636 011, Tamil Nadu, India.
authors.forename: Venugopal
authors.initials: V
authors.lastname: Gopikrishnan
authors.affiliation: Actinobacterial Research Laboratory, Department of Microbiology, Periyar University, Periyar Palkalai Nagar, Salem 636 011, Tamil Nadu, India.
authors.forename: Raasaiyah
authors.initials: R
authors.lastname: Pazhanimurugan
authors.affiliation: Actinobacterial Research Laboratory, Department of Microbiology, Periyar University, Periyar Palkalai Nagar, Salem 636 011, Tamil Nadu, India. Electronic address: rbalaguru@yahoo.com.
authors.forename: Ramasamy
authors.initials: R
authors.lastname: Balagurunathan
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects; D057219:Biofouling / Q000517:prevention & control*; D016923:Cell Death / Q000187:drug effects; D006367:HeLa Cells; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D008826:Microbial Sensitivity Tests; D012834:Silver / Q000494:pharmacology*; D013056:Spectrophotometry, Ultraviolet; D017550:Spectroscopy, Fourier Transform Infrared; D013302:Streptomyces / Q000737:chemistry*
chemical_list: D000900:Anti-Bacterial Agents; D000975:Antioxidants; D012834:Silver
keywords: AFM; Antibacterial; Cytotoxicity; Silver nanoparticle; Streptomyces naganishii