title: The evaluation of 25 chiral stationary phases and the utilization of sub-2.0μm coated polysaccharide chiral stationary phases via supercritical fluid chromatography.
abstract: A rapid screening method to identify the best conditions for chiral separations is described. We analyzed a representative set of 80 racemic compounds against 25 different chiral stationary phases with three different mobile phases to identify the combination of columns and mobile phases that will separate the most compounds on the initial screen. While the OD separated the largest number of compounds, we found the best combination of six columns to be the AD, AS, AY, CC4, ID and Whelk-O1. The second team included the CCC, Cellulose-1, Cellulose-3 or OJ, IA, IE and IF. All 80 compounds were separated with a resolution range of 0.65-15.36. Screening the covalently bonded phases provided separation for 79 of the 80 compounds. We also found ethanol (0.1% NH4OH) separated more compounds than methanol (0.1% NH4OH) or isopropanol (0.1% NH4OH). As part of this study, we also compared the effectiveness of stationary phases that have the same chiral selector. Finally, we demonstrated the effectiveness of using a fast, 1.5-min screening method that utilizes a 1.7μm coated polysaccharide chiral stationary phase.
authors.affiliation: Discovery Chemistry Department, Genentech, Inc., 1 DNA Way, South San Francisco, CA 94080, USA. hamman.christopher@gene.com
authors.forename: Chris
authors.initials: C
authors.lastname: Hamman
authors.forename: Mengling
authors.initials: M
authors.lastname: Wong
authors.forename: Ignacio
authors.initials: I
authors.lastname: Aliagas
authors.forename: Daniel F
authors.initials: DF
authors.lastname: Ortwine
authors.forename: Joseph
authors.initials: J
authors.lastname: Pease
authors.forename: Donald E
authors.initials: DE
authors.lastname: Schmidt
authors.forename: Joseph
authors.initials: J
authors.lastname: Victorino
mesh_terms: D025924:Chromatography, Supercritical Fluid / Q000295:instrumentation* / Q000379:methods; D011134:Polysaccharides / Q000737:chemistry*; D012997:Solvents
chemical_list: D011134:Polysaccharides; D012997:Solvents
keywords: Chiral; Chiral screening; Polysaccharide chiral stationary phases; SFC; Sub-2.0μm particles