title: Bioactive chemical nanopatterns impact human mesenchymal stem cell fate.
abstract: We present a method of preparing and characterizing nanostructured bioactive motifs using a combination of nanoimprint lithography and surface functionalization. Nanodots were fabricated on silicon surfaces and modified with a cell-adhesive RGD peptide for studies in human mesenchymal stem cell adhesion and differentiation. We report that bioactive nanostructures induce mature focal adhesions on human mesenchymal stem cells with an impact on their behavior and dynamics specifically in terms of cell spreading, cell-material contact, and cell differentiation.
authors.affiliation: Institut Européen de la Chimie et Biologie, CBMN-UMR5248, Université de Bordeaux 1, Pessac, France.
authors.forename: Zhe A
authors.initials: ZA
authors.lastname: Cheng
authors.forename: Omar F
authors.initials: OF
authors.lastname: Zouani
authors.forename: Karine
authors.initials: K
authors.lastname: Glinel
authors.forename: Alain M
authors.initials: AM
authors.lastname: Jonas
authors.forename: Marie-Christine
authors.initials: MC
authors.lastname: Durrieu
mesh_terms: D002448:Cell Adhesion; D002454:Cell Differentiation; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000737:chemistry* / Q000166:cytology; D049329:Nanostructures / Q000737:chemistry*; D009842:Oligopeptides / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D009842:Oligopeptides; C047981:arginyl-glycyl-aspartic acid