title: [Inhibition of proliferation of H5N1 subtype AIV in CEF by chemosynthetic siRNA].
abstract: In order to study the proliferation inhibition effect of H5N1 subtype avian influenza virus (AIV) with small interfere RNA (siRNA), a total of 4 siRNAs were designed in accordance with the NP and PA genes of H5N1 subtype AIV, the siRNAs were then transfected to chicken embryo fibroblast(CEF), CEF was infected with H5N1 subtype AIV after 6 hrs. Virus titer of cell supernatant was tested at 16-56hrs post infection, and pathological changes of the cells was observed; mRNA levels of NP, PA, HA and p13-actin gene were tested at 36hrs post infection. The results showed that these 4 siRNAs could inhibit the prolif-eration of H5N1 subtype AIV in CEF in varying degrees, and one siRNA targeting PA was best per-formed. The experimental results also showed that the inhibition effect was decreased with the time prolonged. This research provides a basis for further studying RNAi on AIV prevention and control.
authors.affiliation: Zhuhai Epidemic Prevention and Supervision Center for Animals and Plants, Zhuhai 519015, China. lirushu@163.com
authors.forename: Ru-Shu
authors.initials: RS
authors.lastname: Li
authors.forename: Dan
authors.initials: D
authors.lastname: Yu
authors.forename: Bao-Zheng
authors.initials: BZ
authors.lastname: Luo
authors.forename: Qing-Ru
authors.initials: QR
authors.lastname: Bo
authors.forename: Hai-Nie
authors.initials: HN
authors.lastname: Xu
authors.forename: Cai-Hua
authors.initials: CH
authors.lastname: Sha
authors.forename: Xiu-Yun
authors.initials: XY
authors.lastname: Liao
mesh_terms: D000199:Actins / Q000235:genetics; D000818:Animals; D002642:Chick Embryo; D017931:DNA Primers / Q000235:genetics; D005347:Fibroblasts / Q000821:virology*; D006384:Hemagglutination; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000235:genetics; D006388:Hemagglutinins / Q000235:genetics; D006801:Humans; D053124:Influenza A Virus, H5N1 Subtype / Q000235:genetics / Q000254:growth & development / Q000502:physiology*; D019590:Nucleocapsid Proteins; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000138:chemical synthesis / Q000235:genetics*; D016601:RNA-Binding Proteins / Q000235:genetics; D012324:RNA-Dependent RNA Polymerase / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D013047:Specific Pathogen-Free Organisms; D014162:Transfection; D014758:Viral Core Proteins / Q000235:genetics; D014764:Viral Proteins / Q000235:genetics*; D014779:Virus Replication
chemical_list: D000199:Actins; D017931:DNA Primers; D019267:Hemagglutinin Glycoproteins, Influenza Virus; D006388:Hemagglutinins; C048842:NP protein, Influenza A virus; D019590:Nucleocapsid Proteins; C061290:PA protein, influenza viruses; D034741:RNA, Small Interfering; D016601:RNA-Binding Proteins; D014758:Viral Core Proteins; D014764:Viral Proteins; D012324:RNA-Dependent RNA Polymerase