title: Marininema halotolerans sp. nov., a novel thermoactinomycete isolated from a sediment sample, and emended description of the genus Marininema Li et al. 2012.
abstract: A novel Gram-stain-positive bacterium, designated strain YIM M11385(T), was isolated from a marine sediment sample collected from the South Bay, Little Andaman Island, India with a salinity of 35 p.p.m., pH 8.5. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain YIM M11385(T) belongs to the genus Marininema, supported by a bootstrap value of 100 %. The taxonomic position of this organism was further established by using a polyphasic approach. Strain YIM M11385(T) grew optimally at 28 °C, pH 7.0 and in the presence of 0-5 % (w/v) NaCl. The 16S rRNA gene sequence similarity between strain YIM M11385(T) and Marininema mesophilum SCSIO 10219(T) was 98.3 %. Strain YIM M11385(T) exhibited a quinone system with only MK-7, the polar lipid profile included diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine as major components, and the major fatty acids were anteiso-C15 : 0, iso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0. The level of DNA-DNA relatedness between strain YIM M11385(T) and M. mesophilum SCSIO 10219(T) was 59.36 %. On the basis of genotypic and phenotypic data, it is apparent that strain YIM M11385(T) represents a novel species of the genus Marininema, for which the name Marininema halotolerans sp. nov. is proposed. The type strain is YIM M11385(T) ( = CCTCC AB 2012052(T) = DSM 45789(T)). In addition, we propose that the description of the genus Marininema should be further emended based on the results of the present study.
authors.affiliation: Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education and Laboratory for Conservation and Utilization of Bio-Resources, Yunnan Institute of Microbiology, Yunnan University, Kunming 650091, PR China.
authors.forename: Xiao-Mei
authors.initials: XM
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education and Laboratory for Conservation and Utilization of Bio-Resources, Yunnan Institute of Microbiology, Yunnan University, Kunming 650091, PR China.
authors.forename: Jie
authors.initials: J
authors.lastname: He
authors.affiliation: Key Laboratory of Biogeography and Bioresource in Arid Land, CAS, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Ürűmqi 830011, PR China.|Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education and Laboratory for Conservation and Utilization of Bio-Resources, Yunnan Institute of Microbiology, Yunnan University, Kunming 650091, PR China.
authors.forename: Dao-Feng
authors.initials: DF
authors.lastname: Zhang
authors.affiliation: HongyunHonghe Tobacco (Group) Co., Ltd, Kunming 650202, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Chen
authors.affiliation: Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education and Laboratory for Conservation and Utilization of Bio-Resources, Yunnan Institute of Microbiology, Yunnan University, Kunming 650091, PR China.
authors.forename: Zhao
authors.initials: Z
authors.lastname: Jiang
authors.affiliation: Centre of Advanced Study in Marine Biology, Faculty of Marine Science, Annamalai University, Parangipettai - 608 502, Tamilnadu, India.
authors.forename: Maloy Kumar
authors.initials: MK
authors.lastname: Sahu
authors.affiliation: Centre of Advanced Study in Marine Biology, Faculty of Marine Science, Annamalai University, Parangipettai - 608 502, Tamilnadu, India.
authors.forename: Kannan
authors.initials: K
authors.lastname: Sivakumar
authors.affiliation: Key Laboratory of Biogeography and Bioresource in Arid Land, CAS, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Ürűmqi 830011, PR China.|Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education and Laboratory for Conservation and Utilization of Bio-Resources, Yunnan Institute of Microbiology, Yunnan University, Kunming 650091, PR China.
authors.forename: Wen-Jun
authors.initials: WJ
authors.lastname: Li
mesh_terms: D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D019015:Geologic Sediments / Q000382:microbiology*; D006094:Gram-Positive Bacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D007194:India; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C062629:menaquinone 7