title: Proteomic analysis of tegument-exposed proteins of female and male Schistosoma japonicum worms.
abstract: The interplay between sexes is a prerequisite for female growth, reproductive maturation, and egg production, and the basis of schistosome pathopoiesis and propagation. The tegument is in direct contact with the host environment and its surface membranes are particularly crucial for schistosome survival in the definitive host. In this study, a streptavidin-biotin affinity purification technique combined with LC-MS/MS was used to analyze putative tegument-exposed proteins in female and male adult Schistosoma japonicum worms. In total, 179 proteins were identified in females and 300 in males, including 119 proteins common to both sexes, and 60 female biased and 181 male biased proteins. Some (e.g., serpin and CD36-like class B scavenger receptor) were involved in host-schistosome interactions, while some (e.g., gynecophoral canal protein) were important in the interplay between sexes. Gene Ontology analysis revealed that proteins involved in protein glycosylation and lysosome were highly expressed in females, while proteins involved in intracellular signal transduction, regulation of actin filament polymerization, and proteasome core complex were highly expressed in males. These results might elucidate physiological differences between the sexes. Our study provides new insights into schistosome growth and sexual maturity in the final host and permits the screening of vaccine candidates or drug targets for schistosomiasis.
authors.affiliation: Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Key Laboratory of Animal Parasitology, Ministry of Agriculture of China, Shanghai 200241, P.R. China.
authors.forename: Min
authors.initials: M
authors.lastname: Zhang
authors.forename: Yang
authors.initials: Y
authors.lastname: Hong
authors.forename: Yanhui
authors.initials: Y
authors.lastname: Han
authors.forename: Hongxiao
authors.initials: H
authors.lastname: Han
authors.forename: Jinbiao
authors.initials: J
authors.lastname: Peng
authors.forename: Chunhui
authors.initials: C
authors.lastname: Qiu
authors.forename: Jianmei
authors.initials: J
authors.lastname: Yang
authors.forename: Ke
authors.initials: K
authors.lastname: Lu
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Fu
authors.forename: Jiaojiao
authors.initials: J
authors.lastname: Lin
mesh_terms: D000818:Animals; D002853:Chromatography, Liquid; D019295:Computational Biology; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005786:Gene Expression Regulation / Q000235:genetics*; D063990:Gene Ontology; D008297:Male; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D020543:Proteome / Q000235:genetics* / Q000378:metabolism; D040901:Proteomics / Q000379:methods; D011817:Rabbits / Q000469:parasitology; D012549:Schistosoma japonicum / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D012737:Sex Factors; D012867:Skin / Q000378:metabolism*; D053719:Tandem Mass Spectrometry
chemical_list: D008565:Membrane Proteins; D020543:Proteome