title: Highly sensitive simultaneous detection of lead(II) and barium(II) with G-quadruplex DNA in α-hemolysin nanopore.
abstract: Both Pb(2+) and Ba(2+) can bind with high affinity to some specific DNA sequences, inducing the formation of unimolecular G-quadruplex structures. Translocation of a DNA probe containing such sequences through an α-hemolysin nanopore in the presence of Pb(2+) or Ba(2+) would result in much prolonged DNA translocation events. Quantification of these events can reveal the concentrations of Pb(2+) or Ba(2+) at as low as 0.8 nM. Besides, Pb(2+) and Ba(2+) in the solution can be simultaneously detected and individually identified. Furthermore, the probe is highly selective for Pb(2+) and Ba(2+) detection without interference from other metal ions. This sensing strategy can be extended to many other analytes which can bind to DNA molecules with high affinity.
authors.affiliation: Key Laboratory for Biomedical Effects of Nanomaterials & Nanosafety, Institute of High Energy Physics, Chinese Academy of Sciences, Beijing 100049, China.
authors.forename: Chun
authors.initials: C
authors.lastname: Yang
authors.forename: Lei
authors.initials: L
authors.lastname: Liu
authors.forename: Tao
authors.initials: T
authors.lastname: Zeng
authors.forename: Daowu
authors.initials: D
authors.lastname: Yang
authors.forename: Zhiyi
authors.initials: Z
authors.lastname: Yao
authors.forename: Yuliang
authors.initials: Y
authors.lastname: Zhao
authors.forename: Hai-Chen
authors.initials: HC
authors.lastname: Wu
mesh_terms: D001464:Barium / Q000032:analysis*; D001483:Base Sequence; D015374:Biosensing Techniques / Q000379:methods*; D015342:DNA Probes / Q000737:chemistry* / Q000235:genetics; D004785:Environmental Pollutants / Q000032:analysis; D054856:G-Quadruplexes; D006460:Hemolysin Proteins / Q000737:chemistry*; D007854:Lead / Q000032:analysis*; D058608:Nanopores; D013997:Time Factors
chemical_list: D015342:DNA Probes; D004785:Environmental Pollutants; D006460:Hemolysin Proteins; D001464:Barium; D007854:Lead