title: Functionalized viral nanoparticles as ultrasensitive reporters in lateral-flow assays.
abstract: Two types of viral nanoparticles were functionalized with target-specific antibodies and multiple copies of an enzymatic reporter (horseradish peroxidase). The particles were successfully integrated into an immunochromatographic assay detecting MS2 bacteriophage, a model for viral pathogens. The sensitivity of the assay was greatly superior to conventional gold nanoparticle lateral flow assays, and results could easily be evaluated, even without advanced lab instruments.
authors.affiliation: Department of Biology and Biochemistry, University of Houston, Houston, TX 77204-5001, USA.
authors.forename: Meena
authors.initials: M
authors.lastname: Adhikari
authors.forename: Sagar
authors.initials: S
authors.lastname: Dhamane
authors.forename: Anna E V
authors.initials: AE
authors.lastname: Hagström
authors.forename: Gavin
authors.initials: G
authors.lastname: Garvey
authors.forename: Wen-Hsiang
authors.initials: WH
authors.lastname: Chen
authors.forename: Katerina
authors.initials: K
authors.lastname: Kourentzi
authors.forename: Ulrich
authors.initials: U
authors.lastname: Strych
authors.forename: Richard C
authors.initials: RC
authors.lastname: Willson
mesh_terms: D000595:Amino Acid Sequence; D004664:Encephalitis Virus, Japanese / Q000737:chemistry* / Q000235:genetics; D006735:Horseradish Peroxidase / Q000032:analysis; D006801:Humans; D017909:Levivirus / Q000737:chemistry* / Q000235:genetics; D008969:Molecular Sequence Data; D053758:Nanoparticles / Q000032:analysis*
chemical_list: D006735:Horseradish Peroxidase