title: Characterization of a serologic marker candidate for development of a live-attenuated DIVA vaccine against porcine reproductive and respiratory syndrome virus.
abstract: DIVA (differentiating infected from vaccinated animals) vaccines have proven extremely useful for control and eradication of infectious diseases in livestock. We describe here the characterization of a serologic marker epitope, so-called epitope-M201, which can be a potential target for development of a live-attenuated DIVA vaccine against porcine reproductive and respiratory syndrome virus (PRRSV). Epitope-M201 is located at the carboxyl terminus (residues 161-174) of the viral M protein. The epitope is highly immunodominant and well-conserved among type-II PRRSV isolates. Rabbit polyclonal antibodies prepared against this epitope are non-neutralizing; thus, the epitope does not seem to contribute to the protective immunity against PRRSV infection. Importantly, the immunogenicity of epitope-M201 can be disrupted through the introduction of a single amino acid mutation which does not adversely affect the viral replication. All together, our results provide an important starting point for the development of a live-attenuated DIVA vaccine against type-II PRRSV.
authors.affiliation: Nebraska Center for Virology and School of Veterinary Medicine and Biomedical Sciences, University of Nebraska-Lincoln, Lincoln, NE 68583, USA.
authors.forename: Hiep L X
authors.initials: HL
authors.lastname: Vu
authors.forename: Byungjoon
authors.initials: B
authors.lastname: Kwon
authors.forename: Marcelo
authors.initials: M
authors.lastname: de Lima
authors.forename: Asit K
authors.initials: AK
authors.lastname: Pattnaik
authors.forename: Fernando A
authors.initials: FA
authors.lastname: Osorio
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D000939:Epitopes / Q000097:blood / Q000276:immunology*; D006023:Glycoproteins / Q000276:immunology; D051379:Mice; D019318:Porcine Reproductive and Respiratory Syndrome / Q000276:immunology / Q000517:prevention & control*; D019316:Porcine respiratory and reproductive syndrome virus / Q000235:genetics / Q000276:immunology*; D013552:Swine; D013553:Swine Diseases / Q000276:immunology / Q000517:prevention & control; D014611:Vaccination / Q000662:veterinary*; D014613:Vaccines, Attenuated / Q000276:immunology; D014765:Viral Vaccines / Q000235:genetics / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D000939:Epitopes; D006023:Glycoproteins; D014613:Vaccines, Attenuated; D014765:Viral Vaccines; C023587:protein M (glycoprotein)
keywords: DIVA vaccines; M protein; PRRSV; Serological marker epitope
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