title: EP1: a novel rabbit monoclonal antibody for detection of oestrogen receptor α.
abstract: OBJECTIVE
  Assessment of hormone receptor expression is part of routine examination of every breast cancer. In this study, we report the characterisation of a novel rabbit monoclonal antibody, clone EP1, directed against oestrogen receptor (ER) α. Additionally, its immunohistochemical performance characteristics in archival tissues are evaluated in normal tissues and two distinct cohorts of breast cancer patients.
  
  
  METHODS
  Comparative analyses between EP1 and the anti-ERα component of the ER/PR pharmDx kit (cocktail of mouse monoclonal antibody clones 1D5 and ER-2-123) and between EP1 and another commercially available rabbit monoclonal antibody, clone SP1, are described.
  
  
  RESULTS
  Clone EP1 specifically detects nuclear ER in all tissues examined; cytoplasmic staining was not observed. The analysis shows a high degree of concordance (~95%) between EP1 and both the ERα component of the Dako ER/PR pharmDx kit and Ventana clone SP1. However, the use of EP1 antibody together with Dako EnVision FLEX detection system resulted in a stronger staining intensity as compared with SP1 antibody using the Ventana ultraView DAB detection system resulting in better 'ease of use.'
  
  
  CONCLUSIONS
  The use of EPI can result in better interpretation of the results of the ER analysis.
authors.affiliation: Department of Pathology and Laboratory Medicine, Indiana University School of Medicine, , Indianapolis, Indiana, USA.
authors.forename: Sunil
authors.initials: S
authors.lastname: Badve
authors.forename: I Tudor
authors.initials: IT
authors.lastname: Vladislav
authors.forename: Betsy
authors.initials: B
authors.lastname: Spaulding
authors.forename: Anna
authors.initials: A
authors.lastname: Strickland
authors.forename: Sylvia
authors.initials: S
authors.lastname: Hernandez
authors.forename: Lisa
authors.initials: L
authors.lastname: Bird-Turner
authors.forename: Cecelia
authors.initials: C
authors.lastname: Dodson
authors.forename: Bjorn
authors.initials: B
authors.lastname: Elleby
authors.forename: Therese
authors.initials: T
authors.lastname: Phillips
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D014408:Biomarkers, Tumor / Q000032:analysis* / Q000378:metabolism; D001943:Breast Neoplasms / Q000378:metabolism / Q000473:pathology*; D015331:Cohort Studies; D018604:Epitope Mapping; D047628:Estrogen Receptor alpha / Q000032:analysis* / Q000276:immunology / Q000378:metabolism; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry; D051379:Mice; D008969:Molecular Sequence Data; D011817:Rabbits; D011933:Reagent Kits, Diagnostic
chemical_list: D000911:Antibodies, Monoclonal; D014408:Biomarkers, Tumor; C506487:ESR1 protein, human; D047628:Estrogen Receptor alpha; D011933:Reagent Kits, Diagnostic
keywords: Antibodies; Breast Cancer; Immunohistochemistry
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