title: Hepcidin expression from monocyte of splenectomized and non-splenectomized patients with HbE-β-thalassemia.
abstract: OBJECTIVE
  Hepcidin is a key regulator of body iron homeostasis. The inflammatory cytokine interleukin (IL)-6 has been reported to upregulate expression of the hepcidin (HAMP) gene in monocytes. The purpose of this work was to determine HAMP expression at steady state in monocytes of splenectomized and non-splenectomized patients with HbE-β-thalassemia compared with normal controls.
  
  
  METHODS
  Levels of HAMP mRNA were measured using real-time reverse transcriptase polymerase chain reaction. Plasma IL-6, soluble transferrin receptor (sTfR), and ferritin levels were determined by enzyme-linked immunosorbent assay, and C-reactive protein (CRP) by nephelometry.
  
  
  RESULTS
  Levels of HAMP mRNA, CRP, IL-6, sTfR, and ferritin were significantly higher in both groups of patients with thalassemia than controls, but were not different between splenectomized and non-splenectomized patients. Monocyte HAMP mRNA content of patients with thalassemia correlated with plasma IL-6 and CRP levels.
  
  
  CONCLUSIONS
  Patients with HbE-β-thalassemia have persistent elevation of the plasma inflammatory cytokines, CRP, and IL-6, and the latter could be responsible (in part) to the induction of HAMP expression in monocytes of patients with HbE-β-thalassemia.
authors.forename: Kanita
authors.initials: K
authors.lastname: Pratummo
authors.forename: Arunee
authors.initials: A
authors.lastname: Jetsrisuparb
authors.forename: Supan
authors.initials: S
authors.lastname: Fucharoen
authors.forename: Amporn
authors.initials: A
authors.lastname: Tripatara
mesh_terms: D000293:Adolescent; D000328:Adult; D002097:C-Reactive Protein / Q000378:metabolism; D002648:Child; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005293:Ferritins / Q000097:blood; D015870:Gene Expression; D006446:Hemoglobin E / Q000235:genetics*; D064451:Hepcidins / Q000235:genetics*; D006801:Humans; D015850:Interleukin-6 / Q000097:blood; D008297:Male; D009000:Monocytes / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011990:Receptors, Transferrin / Q000097:blood; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013156:Splenectomy; D055815:Young Adult; D017086:beta-Thalassemia / Q000097:blood / Q000235:genetics*
chemical_list: D064451:Hepcidins; D015850:Interleukin-6; D012333:RNA, Messenger; D011990:Receptors, Transferrin; D002097:C-Reactive Protein; D005293:Ferritins; D006446:Hemoglobin E
keywords: Hepcidin; IL-6; Monocyte; Splenectomy; Thalassemia