title: A xenograft mantle transplantation technique for producing a novel pearl in an akoya oyster host.
abstract: The brightness and color of pearls varies among different pearl-producing shellfish and have been a source of human fascination since ancient times. When produced through cultivation, the characteristics and quality of a pearl depend on the kind of shellfish used and also the transplanted mantle graft. This suggests that the Akoya pearl oyster, which is generally used in Japan for pearl culturing, can produce different kinds of pearl through the use of mantles from different species of shellfish. However, a transplanted heterogeneous mantle would be rejected by the immune system of the Akoya oyster. We have therefore developed a new method to suppress the Akoya immune system that archives immune tolerance to other shellfish. It is generally known that small quantities of antigens can be used to produce archived immunological tolerance in a clinical setting. We successfully suppressed the Akoya pearl oyster immune response against a Mabé pearl oyster graft through repeat injections of mantle homogenates. We then transplanted a Mabé pearl oyster mantle graft into the immunologically tolerant Akoya pearl oyster and obtained a Mabé pearl from an Akoya pearl oyster. Our new technique thus makes the production of novel and different pearls in the Akoya possible. We believe that this has significant future potential for the advancement of the pearl industry.
authors.affiliation: Research Group for Reproductive Physiology, South Ehime Fisheries Research Center, Ehime University, Ainan, Ehime, Japan.
authors.forename: Ei
authors.initials: E
authors.lastname: Fukushima
authors.forename: Toshiharu
authors.initials: T
authors.lastname: Iwai
authors.forename: Chiemi
authors.initials: C
authors.lastname: Miura
authors.forename: Fritzie T
authors.initials: FT
authors.lastname: Celino
authors.forename: Shintarou
authors.initials: S
authors.lastname: Urasaki
authors.forename: Takeshi
authors.initials: T
authors.lastname: Miura
mesh_terms: D060105:Animal Shells / Q000737:chemistry*; D000825:Animal Structures / Q000276:immunology / Q000637:transplantation*; D000818:Animals; D017756:Aquaculture / Q000379:methods*; D017931:DNA Primers / Q000235:genetics; D005434:Flow Cytometry; D005453:Fluorescence; D007165:Immunosuppression Therapy / Q000379:methods; D010587:Phagocytosis / Q000502:physiology; D049911:Pinctada / Q000737:chemistry* / Q000276:immunology*; D016133:Polymerase Chain Reaction; D013045:Species Specificity; D014183:Transplantation, Heterologous
chemical_list: D017931:DNA Primers
references.citation: Dev Comp Immunol. 1992 Mar-Jun;16(2-3):123-38
references.pmid: 1499833
references.citation: Dev Comp Immunol. 1990 Spring;14(2):161-73
references.pmid: 2369965
references.citation: Fish Shellfish Immunol. 2010 Jul;29(1):126-35
references.pmid: 20211734
references.citation: Mar Biotechnol (NY). 2007 Jan-Feb;9(1):113-6
references.pmid: 17139560
references.citation: Allergy. 2011 Jun;66(6):713-24
references.pmid: 21320133