title: In vitro and in vivo evaluation of SU-8 biocompatibility.
abstract: SU-8 negative photoresist is a high tensile strength polymer that has been used for a number of biomedical applications that include cell encapsulation and neuronal probes. Chemically, SU-8 comprises, among other components, an epoxy based monomer and antimony salts, the latter being a potential source of cytotoxicity. We report on the in vitro and in vivo evaluation of SU-8 biocompatibility based on leachates from various solvents, at varying temperatures and pH, and upon subcutaneous implantation of SU-8 substrates in mice. MTT cell viability assay did not exhibit any cytotoxic effects from the leachates. The hemolytic activity of SU-8 is comparable to that of FDA approved implant materials such as silicone elastomer, Buna-S and medical steel. In vivo histocompatibility study in mice indicates a muted immune response to subcutaneous SU-8 implants.
authors.affiliation: Department of Radiology, Geisel School of Medicine at Dartmouth, Hanover, NH 03756, USA.
authors.forename: Krishnamurthy V
authors.initials: KV
authors.lastname: Nemani
authors.forename: Karen L
authors.initials: KL
authors.lastname: Moodie
authors.forename: Jeoffry B
authors.initials: JB
authors.lastname: Brennick
authors.forename: Alison
authors.initials: A
authors.lastname: Su
authors.forename: Barjor
authors.initials: B
authors.lastname: Gimi
mesh_terms: D000362:Agar; D000818:Animals; D000965:Antimony / Q000032:analysis; D001672:Biocompatible Materials / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D004852:Epoxy Compounds / Q000737:chemistry / Q000494:pharmacology*; D006461:Hemolysis / Q000187:drug effects; D007109:Immunity / Q000187:drug effects; D020007:Implants, Experimental; D008297:Male; D008422:Materials Testing; D051379:Mice; D008807:Mice, Inbred BALB C; D009928:Organ Specificity / Q000187:drug effects; D011108:Polymers / Q000737:chemistry / Q000494:pharmacology*; D019919:Prosthesis Implantation; D051381:Rats; D013054:Spectrophotometry, Atomic; D013194:Staining and Labeling
chemical_list: D001672:Biocompatible Materials; D004852:Epoxy Compounds; D011108:Polymers; C520895:SU-8 compound; D000362:Agar; D000965:Antimony
keywords: Biocompatibility; Cytotoxicity; Histocompatibility; SU-8
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