title: Understanding the relevance of local conformational stability and dynamics to the aggregation propensity of an IgG1 and IgG2 monoclonal antibodies.
abstract: Aggregation of monoclonal antibodies is often a multi-step process involving structural alterations in monomeric proteins and subsequent formation of soluble or insoluble oligomers. The role of local conformational stability and dynamics of native and/or partially altered structures in determining the aggregation propensity of monoclonal antibodies, however, is not well understood. Here, we investigate the role of conformational stability and dynamics of regions with distinct solvent exposure in determining the aggregation propensity of an IgG1 and IgG2 monoclonal antibody. The temperatures employed span the pre-unfolding range (10-40°C) and the onset temperatures (T onset ) for exposure of apolar residues (≈ 50°C), alterations in secondary structures (≈ 60°C) and initiation of visible aggregate formation (≈ 60°C). Solvent-exposed regions were found to precede solvent-shielded regions in an initiation of aggregation for both proteins. Such a process was observed upon alterations in overall tertiary structure while retaining the secondary structures in both the proteins. In addition, a greater dynamic nature of solvent-shielded regions in potential intermediates of IgG1 and the improved conformational stability increased its resistance to aggregation when compared to IgG2. These results suggest that local conformational stability and fluctuations of partially altered structures can influence the aggregation propensity of immunoglobulins.
authors.affiliation: Department of Pharmaceutical Chemistry, Macromolecule and Vaccine Stabilization Center, University of Kansas, Lawrence, Kansas, 66047, USA.
authors.forename: Santosh V
authors.initials: SV
authors.lastname: Thakkar
authors.forename: Neha
authors.initials: N
authors.lastname: Sahni
authors.forename: Sangeeta B
authors.initials: SB
authors.lastname: Joshi
authors.forename: Bruce A
authors.initials: BA
authors.lastname: Kerwin
authors.forename: Feng
authors.initials: F
authors.lastname: He
authors.forename: David B
authors.initials: DB
authors.lastname: Volkin
authors.forename: C Russell
authors.initials: CR
authors.lastname: Middaugh
mesh_terms: D020106:Acrylamide / Q000494:pharmacology; D000911:Antibodies, Monoclonal / Q000737:chemistry*; D002942:Circular Dichroism; D005453:Fluorescence; D007074:Immunoglobulin G / Q000737:chemistry*; D007700:Kinetics; D046912:Multiprotein Complexes / Q000737:chemistry; D011487:Protein Conformation; D017510:Protein Folding; D055503:Protein Multimerization; D055550:Protein Stability; D013696:Temperature; D014364:Tryptophan / Q000737:chemistry
chemical_list: D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G; D046912:Multiprotein Complexes; D020106:Acrylamide; D014364:Tryptophan
keywords: aggregation; circular dichroism; conformational stability; differential scanning calorimetry; dynamics; fluorescence spectroscopy; light scattering; monoclonal antibody
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