title: Variation in the transmission of barley yellow dwarf virus-PAV by different Sitobion avenae clones in China.
abstract: Fourteen Sitobion avenae Fabricius (Hemiptera: Aphididae) clonal lines (clones) originating from China were tested for their ability to transmit BYDV-PAV (one isolate from Belgium and another from China) using wheat plants. By sequence analysis, the coat protein gene of BYDV-PAV-BE was distinguishable from BYDV-PAV-CN. All of the clones could transmit BYDV-PAV, and the transmission varied from 24.42% to 66.67% with BYDV-PAV-BE and from 23.55% to 56.18% with BYDV-PAV-CN. These data suggest that S. avenae has no specialty in BYDV-PAV isolate. Significant differences in the transmission frequencies between the clones with BYDV-PAV-BE and BYDV-PAV-CN were observed. The transmission efficiencies of aphid clones from the middle-lower reaches of Yangtze River (AH, HD, HDE, HZ, JZ, JY and SJ) and Yunnan province (YH) were similar. Nevertheless, differences in the virus transmission efficiencies of the clones from northern (ST and STA) and northwestern (QX, SB and XS) regions were assessed. The transmission efficiency of S. avenae from northern and northwestern China, where BYDV impact is more important, was higher than that from the middle-lower reaches of the Yangtze River and Yunnan province. This work emphasizes the importance of considering aphid vector clonal diversity in addition to virus strain variability when assessing BYDV transmission efficiency.
authors.affiliation: State Key Laboratory for Biology of Plant Disease and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China; Functional and Evolutionary Entomology, Gembloux Agro-Bio Tech, University of Liege, Passage des Déportés 2, B-5030 Gembloux, Belgium.
authors.forename: Wenjuan
authors.initials: W
authors.lastname: Yu
authors.forename: Zhaohuan
authors.initials: Z
authors.lastname: Xu
authors.forename: Frédéric
authors.initials: F
authors.lastname: Francis
authors.forename: Yong
authors.initials: Y
authors.lastname: Liu
authors.forename: Dengfa
authors.initials: D
authors.lastname: Cheng
authors.forename: Claude
authors.initials: C
authors.lastname: Bragard
authors.forename: Julian
authors.initials: J
authors.lastname: Chen
mesh_terms: D000818:Animals; D001042:Aphids / Q000821:virology*; D001530:Belgium; D002681:China; D001467:Hordeum / Q000821:virology*; D017793:Luteovirus / Q000302:isolation & purification*; D010935:Plant Diseases / Q000821:virology*
keywords: Barley yellow dwarf virus; RT-PCR; Sitobion avenae; TAS-ELISA; Transmission efficiency