title: No overt structural or functional changes associated with PEG-coated gold nanoparticles accumulation with acute exposure in the mouse heart.
abstract: In this study, we investigated the cardiac biodistribution of polyethylene glycol (PEG)-coated AuNPs and their effects on cardiac function, structure and inflammation in both normal and cardiac remodeling mice. The model of cardiac remodeling was induced by subcutaneously injection of isoproterenol (ISO), a non-selective beta-adrenergic agonist, for 7 days. After AuNPs were injected intravenously in mice for 7 consecutive days, Au content in different organs was determined quantitatively by inductively coupled plasma mass spectrometry (ICP-MS), cardiac function and structure were measured by echocardiography, cardiac fibrosis was examined with picrosirius red staining, the morphology of cardiomyocytes was observed with hematoxylin and eosin (H & E) staining. The accumulation of AuNPs in hearts did not affect cardiac function or induce cardiac hypertrophy, cardiac fibrosis and cardiac inflammation under normal physiological condition. Cardiac AuNPs content was 6-fold higher in the cardiac remodeling mouse than normal mice. However, the increased accumulation of AuNPs in the heart did not aggravate ISO-induced cardiac hypertrophy, cardiac fibrosis or cardiac inflammation. These observations suggest that PEG-coated AuNPs possess excellent biocompatibility under both physiological and pathological conditions. Thus, AuNPs may be safe for cardiac patients and hold great promise for further development for various biomedical applications.
authors.affiliation: Institute of Vascular Medicine, Peking University Third Hospital, Key Laboratory of Cardiovascular Molecular Biology and Regulatory Peptides, Ministry of Health, Key Laboratory of Molecular Cardiovascular Sciences, Ministry of Education and Beijing Key Laboratory of Cardiovasicular Receptors Research, Beijing 100191, China.
authors.forename: Chengzhi
authors.initials: C
authors.lastname: Yang
authors.forename: Hui
authors.initials: H
authors.lastname: Yang
authors.forename: Jimin
authors.initials: J
authors.lastname: Wu
authors.forename: Zenghui
authors.initials: Z
authors.lastname: Meng
authors.forename: Rui
authors.initials: R
authors.lastname: Xing
authors.forename: Aiju
authors.initials: A
authors.lastname: Tian
authors.forename: Xin
authors.initials: X
authors.lastname: Tian
authors.forename: Lijun
authors.initials: L
authors.lastname: Guo
authors.forename: Youyi
authors.initials: Y
authors.lastname: Zhang
authors.forename: Guangjun
authors.initials: G
authors.lastname: Nie
authors.forename: Zijian
authors.initials: Z
authors.lastname: Li
mesh_terms: D000318:Adrenergic beta-Agonists; D000818:Animals; D006332:Cardiomegaly / Q000139:chemically induced / Q000276:immunology / Q000473:pathology / Q000503:physiopathology; D054715:Cardiotoxins / Q000009:adverse effects; D004195:Disease Models, Animal; D016503:Drug Delivery Systems; D005355:Fibrosis; D006046:Gold / Q000009:adverse effects* / Q000032:analysis / Q000493:pharmacokinetics; D006352:Heart Ventricles / Q000166:cytology / Q000187:drug effects* / Q000276:immunology / Q000473:pathology; D007545:Isoproterenol; D008297:Male; D053768:Metal Nanoparticles / Q000009:adverse effects* / Q000737:chemistry / Q000648:ultrastructure; D051379:Mice; D008807:Mice, Inbred BALB C; D011092:Polyethylene Glycols / Q000009:adverse effects; D011897:Random Allocation; D013499:Surface Properties; D014018:Tissue Distribution; D016276:Ventricular Function / Q000187:drug effects*; D020257:Ventricular Remodeling / Q000187:drug effects
chemical_list: D000318:Adrenergic beta-Agonists; D054715:Cardiotoxins; D011092:Polyethylene Glycols; D006046:Gold; D007545:Isoproterenol
keywords: Cardiac remodeling; Cardiac toxicity; Gold nanoparticles; Heart