title: Aggregatibacter actinomycetemcomitans lipopolysaccharide stimulated epithelial cells produce interleukin-15 that regulates T cell activation.
abstract: OBJECTIVE
  Oral epithelial cells act not only as mechanical barriers but also as immunological barriers by producing various mediators such as cytokines. Since, in periodontal disease, limited information is available regarding the role of oral epithelial cell-derived cytokines on T cell activation, we investigated the responses of human T cells (Jurkat cell) to cytokines in KB cells (an oral epithelial cell line) that had been stimulated with Aggregatibacter actinomycetemcomitans lipopolysaccharide (LPS).
  
  
  METHODS
  To evaluate T cell activation in response to the culture supernatant of KB cells, we examined cell proliferation and interferon gamma (IFN-γ) production, which is closely related to periodontal disease, in Jurkat cells. Culture supernatant of LPS-stimulated KB cells enhanced cell proliferation and IFN-γ production in Jurkat cells. To determine the active component within the culture supernatant, the production of epithelial cell-derived cytokines, interleukin-12 (IL-12), IL-15 and IL-18, in LPS-stimulated KB cells was analysed.
  
  
  RESULTS
  IL-15, but not IL-18, was significantly increased in the culture supernatant of LPS-stimulated KB cells. Moreover, additional anti-IL-15 neutralizing antibody abolished culture supernatant-induced IFN-γ expression in Jurkat cells.
  
  
  CONCLUSIONS
  These results suggest that periodontal pathogens induce the production of IL-15 from epithelial cells, and leading the activation of T cells in periodontal lesions.
authors.affiliation: Department of Periodontology, School of Dentistry, Aichi Gakuin University, 2-11 Suemoridori, Chikusa-ku, Nagoya, Aichi 464-8651, Japan.
authors.forename: Takayuki
authors.initials: T
authors.lastname: Suga
authors.forename: Akio
authors.initials: A
authors.lastname: Mitani
authors.forename: Makio
authors.initials: M
authors.lastname: Mogi
authors.forename: Takeshi
authors.initials: T
authors.lastname: Kikuchi
authors.forename: Takeki
authors.initials: T
authors.lastname: Fujimura
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Takeda
authors.forename: Toshimitsu
authors.initials: T
authors.lastname: Hishikawa
authors.forename: Genta
authors.initials: G
authors.lastname: Yamamoto
authors.forename: Jun-Ichiro
authors.initials: J
authors.lastname: Hayashi
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Ishihara
authors.forename: Toshihide
authors.initials: T
authors.lastname: Noguchi
mesh_terms: D016976:Aggregatibacter actinomycetemcomitans / Q000276:immunology*; D049109:Cell Proliferation; D002478:Cells, Cultured; D004797:Enzyme-Linked Immunosorbent Assay; D004847:Epithelial Cells / Q000276:immunology*; D006801:Humans; D007371:Interferon-gamma / Q000276:immunology; D018664:Interleukin-12 / Q000276:immunology; D019409:Interleukin-15 / Q000276:immunology*; D020382:Interleukin-18 / Q000276:immunology; D019169:Jurkat Cells; D007624:KB Cells; D008070:Lipopolysaccharides; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D019409:Interleukin-15; D020382:Interleukin-18; D008070:Lipopolysaccharides; D018664:Interleukin-12; D007371:Interferon-gamma
keywords: Aggregatibacter actinomycetemcomitans (Actinobacillus actinomycetemcomitans); Epithelial cells; Interferon-gamma; Interleukin-15; Periodontal diseases