title: Enzymes involved in vinyl acetate decomposition by Pseudomonas fluorescens PCM 2123 strain.
abstract: Esterases are widely used in food processing industry, but there is little information concerning enzymes involved in decompositions of esters contributing to pollution of environment. Vinyl acetate (an ester of vinyl alcohol and acetic acid) is a representative of volatile organic compounds (VOCs) in decomposition, of which hydrolyses and oxidoreductases are mainly involved. Their activities under periodically changing conditions of environment are essential for the removal of dangerous VOCs. Esterase and alcohol/aldehyde dehydrogenase activities were determined in crude cell extract from Pseudomonas fluorescens PMC 2123 after vinyl acetate induction. All examined enzymes exhibit their highest activity at 30-35 °C and pH 7.0-7.5. Esterase preferably hydrolyzed ester bonds with short fatty chains without plain differences for C2 or C4. Comparison of Km values for alcohol and aldehyde dehydrogenases for acetaldehyde suggested that this metabolite was preferentially oxidized than reduced. Activity of alcohol dehydrogenase reducing acetaldehyde to ethanol suggested that one mechanism of defense against the elevated concentration of toxic acetaldehyde could be its temporary reduction to ethanol. Esterase activity was inhibited by phenylmethanesulfonyl fluoride, while β-mercaptoethanol, dithiothreitol, and ethylenediaminetetraacetic acid had no inhibitor effect. From among metal ions, only Mg(2+) and Fe(2+) stimulated the cleavage of ester bond.
authors.affiliation: Institute of Chemical Engineering, Polish Academy of Sciences, Bałtycka 5, 44-100, Gliwice, Poland.
authors.forename: Elżbieta
authors.initials: E
authors.lastname: Szczyrba
authors.forename: Izabela
authors.initials: I
authors.lastname: Greń
authors.forename: Grażyna
authors.initials: G
authors.lastname: Bartelmus
mesh_terms: D001711:Biotransformation; D020536:Enzyme Activators / Q000032:analysis; D004791:Enzyme Inhibitors / Q000032:analysis; D004795:Enzyme Stability; D004950:Esterases / Q000737:chemistry / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D010088:Oxidoreductases / Q000737:chemistry / Q000378:metabolism*; D011551:Pseudomonas fluorescens / Q000201:enzymology* / Q000378:metabolism*; D013696:Temperature; D014753:Vinyl Compounds / Q000378:metabolism*
chemical_list: D020536:Enzyme Activators; D004791:Enzyme Inhibitors; D014753:Vinyl Compounds; D010088:Oxidoreductases; D004950:Esterases; C011566:vinyl acetate
references.citation: Protein Expr Purif. 2006 Feb;45(2):315-23
references.pmid: 16061395
references.citation: J Dairy Sci. 2003 Aug;86(8):2547-57
references.pmid: 12939078
references.citation: Enzyme Microb Technol. 2008 Jan;42(2):181-6
references.pmid: 22578869
references.citation: Environ Technol. 2010 Jan;31(1):1-6
references.pmid: 20232673
references.citation: Appl Environ Microbiol. 2004 Jun;70(6):3213-21
references.pmid: 15184114
references.citation: Extremophiles. 2011 Mar;15(2):203-11
references.pmid: 21181486
references.citation: J Dairy Sci. 1997 Dec;80(12):3099-106
references.pmid: 9436090
references.citation: J Dairy Sci. 2003 Apr;86(4):1118-29
references.pmid: 12741535
references.citation: Appl Environ Microbiol. 1990 Oct;56(10):3023-8
references.pmid: 2285314
references.citation: Biochem J. 1988 Mar 1;250(2):453-8
references.pmid: 3128284
references.citation: Ann Microbiol. 2011 Jun;61(2):257-265
references.pmid: 21654921
references.citation: Anal Biochem. 1976 May 7;72:248-54
references.pmid: 942051