title: Adult Opisthorchis felineus major protein fractions deduced from transcripts: comparison with liver flukes Opisthorchis viverrini and Clonorchis sinensis.
abstract: The epidemiologically important liver flukes Opisthorchis felineus, Opisthorchis viverrini, and Clonorchis sinensis are of interest to health professionals, epidemiologists, pharmacologists, and molecular biologists. Recently the transcriptomes of the latter two species were intensively investigated. However our knowledge on molecular biology of O. felineus is scarce. We report the first results of the O. felineus transcriptome analysis. We isolated and annotated a total of 2560 expressed sequence tag (EST) sequences from adult O. felineus (deposited within the database of expressed sequence tags (dbEST), under accession numbers GenBank: JK624271-JK626790, JK006511-JK006547, JK649790-JK649792). Clustering and analysis resulted in the detection of 267 contigs. Of the protein sequences deduced from these, 82% had homologs in the NCBI (nr) protein database and 63% contained conserved domains, allowing the functions to be interpreted using the Gene Ontology terms. Comprehensive analysis of Opisthorchiidae- and Trematoda-specific substitutions within amino acid sequences deduced for the proteins myoglobin, vitelline precursor protein, cathepsin F, and 28kDa glutathione transferase was carried out. The gene set of the 32 ribosomal proteins for the three Opisthorchiidae species with the addition of available Schistosoma and Fasciola orthologs was created and is provided in the supplementary. The orthologous gene set created was used for inferring phylogeny within the Trematoda with special attention to interrelations within the Opisthorchiidae. The phylogenetic analysis revealed a closer relationship between C. sinensis and O. viverrini and some divergence of O. felineus from either O. viverrini or C. sinensis.
authors.affiliation: Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences, pr. Lavrentieva 10, Novosibirsk 630090, Russia. Electronic address: pomaznoy@bionet.nsc.ru.
authors.forename: Mikhail
authors.initials: M
authors.lastname: Pomaznoy
authors.forename: Sergey
authors.initials: S
authors.lastname: Tatkov
authors.forename: Alexey
authors.initials: A
authors.lastname: Katokhin
authors.forename: Dmitry
authors.initials: D
authors.lastname: Afonnikov
authors.forename: Vladimir
authors.initials: V
authors.lastname: Babenko
authors.forename: Dagmara
authors.initials: D
authors.lastname: Furman
authors.forename: Ilya
authors.initials: I
authors.lastname: Brusentsov
authors.forename: Pavel
authors.initials: P
authors.lastname: Belavin
authors.forename: Alexandr
authors.initials: A
authors.lastname: Najakshin
authors.forename: Sergey
authors.initials: S
authors.lastname: Guselnikov
authors.forename: Gennady
authors.initials: G
authors.lastname: Vasiliev
authors.forename: Anton
authors.initials: A
authors.lastname: Sivkov
authors.forename: Egor
authors.initials: E
authors.lastname: Prokhortchouk
authors.forename: Konstantin
authors.initials: K
authors.lastname: Skryabin
authors.forename: Viatcheslav
authors.initials: V
authors.lastname: Mordvinov
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003004:Clonorchis sinensis / Q000737:chemistry* / Q000145:classification / Q000235:genetics; D020451:Contig Mapping; D006224:Cricetinae; D003530:Cyprinidae / Q000469:parasitology; D057056:Cysteine Proteases / Q000737:chemistry / Q000235:genetics; D004527:Egg Proteins / Q000737:chemistry / Q000235:genetics; D020224:Expressed Sequence Tags / Q000737:chemistry; D005393:Fish Diseases / Q000469:parasitology; D005982:Glutathione Transferase / Q000737:chemistry / Q000235:genetics; D015801:Helminth Proteins / Q000737:chemistry* / Q000235:genetics; D008647:Mesocricetus; D058977:Molecular Sequence Annotation; D008969:Molecular Sequence Data; D009211:Myoglobin / Q000737:chemistry / Q000235:genetics; D009889:Opisthorchiasis / Q000469:parasitology / Q000662:veterinary; D009891:Opisthorchis / Q000737:chemistry* / Q000145:classification / Q000235:genetics; D010802:Phylogeny; D011498:Protein Precursors / Q000737:chemistry / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000302:isolation & purification; D012269:Ribosomal Proteins / Q000737:chemistry / Q000235:genetics; D059467:Transcriptome / Q000235:genetics*
chemical_list: D004527:Egg Proteins; D015801:Helminth Proteins; D009211:Myoglobin; D011498:Protein Precursors; D012333:RNA, Messenger; D012269:Ribosomal Proteins; D005982:Glutathione Transferase; D057056:Cysteine Proteases
keywords: EST; Opisthorchis felineus; Phylogeny; Transcriptomics