title: Development of novel amphiphilic magnetic molecularly imprinted polymers compatible with biological fluids for solid phase extraction and physicochemical behavior study.
abstract: In the present work, a novel amphiphilic magnetic molecularly imprinted polymer (M-MIP) has been synthesized by a simple non covalent method for the loading of gatifloxacin (GTFX) in polar solvent. This nanomaterial used as sorbent has been applied to the solid phase extraction of GTFX in different spiked biological fluids. For the first time, studies of dispersibility and solubility behaviors with different solvents and water were performed to demonstrate amphiphilicity and also to find the better nanomaterial obtained. Fourier transform infrared (FT-IR), scanning electron microscopy (SEM), transmission electron microscopy (TEM) and X-ray (XRD) were used to characterize the nanomaterials, and Scatchard plot analysis to demonstrate the binding kinetic. Results suggest that the dispersibility, solubility and the adsorption in water have relationships with the structure of nanomaterials prepared. The oleic acid coated on the M-MIP combined with the washing process has enhanced the amphiphilicity of the nanomaterials. The M-MIP2 showed better selectivity and adsorption behavior with imprinted efficiency higher than (2) in water, as well as in biological fluids. Moreover, no interference with constituents of blank urine and blank serum samples for solid phase extraction (SPE) was observed. Moreover, loading recovery was found higher than 95% with low RSD. The novel amphiphilic magnetic nanomaterial prepared here as sorbent is suitable for SPE of GTFX in biological fluids for therapeutic monitoring control. It could be also used as carrier in drug delivery system for experimental and clinical studies.
authors.affiliation: Department of Analytical Chemistry, China Pharmaceutical University, Nanjing 210009, China.
authors.forename: Pierre
authors.initials: P
authors.lastname: Dramou
authors.forename: Pengli
authors.initials: P
authors.lastname: Zuo
authors.forename: Hua
authors.initials: H
authors.lastname: He
authors.forename: Lien Ai
authors.initials: LA
authors.lastname: Pham-Huy
authors.forename: Wenyue
authors.initials: W
authors.lastname: Zou
authors.forename: Deli
authors.initials: D
authors.lastname: Xiao
authors.forename: Chuong
authors.initials: C
authors.lastname: Pham-Huy
mesh_terms: D055598:Chemical Phenomena; D024841:Fluoroquinolones / Q000097:blood / Q000652:urine; D000077734:Gatifloxacin; D006586:Hexanes; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D054802:Molecular Imprinting / Q000379:methods*; D019301:Oleic Acid; D011108:Polymers; D052616:Solid Phase Extraction / Q000379:methods*; D014867:Water
chemical_list: D024841:Fluoroquinolones; D006586:Hexanes; D058185:Magnetite Nanoparticles; D011108:Polymers; D014867:Water; D019301:Oleic Acid; D000077734:Gatifloxacin
keywords: Amphiphilic magnetic-imprinted polymers; Aqueous media; Biological fluids; Physicochemical behavior study; Solid phase extraction