title: Physicochemical and in vitro biocompatibility evaluation of water-soluble CdSe/ZnS core/shell.
abstract: Group II-VI semiconductor quantum dots (Q-dots) have found various applications in biomedical field during last decade. In this study, we have synthesized CdSe Q-dots and CdSe/ZnS core/shell (CS) by wet chemical route and characterized using X-ray diffraction (XRD), transmission electron microscopy (TEM), dynamic light scattering (DLS), Fourier-transform infrared spectroscopy (FT-IR) and Photoluminescence (PL) spectroscopy. CS formation was confirmed by red shift as well as enhancement in the luminescence peak compared to bare Q-dots. Processing parameters such as core and sulfur concentrations were optimized at maximum luminescence efficiency during the shell preparation. Effects of dialysis, aging and cell culture medium on the properties of the Q-dots and CS were also studied by luminescence and DLS techniques. DLS data showed Q-dots and CS to be stable, and there was no effect on the integrity of the Q-dots and CS after various modifications. CS was found to be hemocompatible and cytocompatible for human umbilical vein endothelial cells even at a high concentration of 0.1 mg/ml up to 48 h indicating high potential for CS in various biomedical applications.
authors.affiliation: 1Dental Products Laboratory, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Trivandrum, India.
authors.forename: Diksha
authors.initials: D
authors.lastname: Painuly
authors.forename: Anugya
authors.initials: A
authors.lastname: Bhatt
authors.forename: Venkiteswaran Kalliyana
authors.initials: VK
authors.lastname: Krishnan
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry* / Q000633:toxicity; D019187:Cadmium Compounds / Q000737:chemistry*; D002470:Cell Survival / Q000187:drug effects; D055598:Chemical Phenomena; D006461:Hemolysis / Q000187:drug effects; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D008163:Luminescent Measurements; D008422:Materials Testing; D046529:Microscopy, Electron, Transmission; D015539:Platelet Activation / Q000187:drug effects; D033362:Powder Diffraction; D045663:Quantum Dots / Q000737:chemistry* / Q000633:toxicity / Q000648:ultrastructure; D012542:Scattering, Radiation; D018036:Selenium Compounds / Q000737:chemistry*; D012995:Solubility; D017550:Spectroscopy, Fourier Transform Infrared; D013440:Sulfides / Q000737:chemistry*; D014867:Water; D017967:Zinc Compounds / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D019187:Cadmium Compounds; D018036:Selenium Compounds; D013440:Sulfides; D017967:Zinc Compounds; D014867:Water; C058667:cadmium selenide; C031238:zinc sulfide
keywords: HUVEC cells; Quantum dots; core/shell; cytotoxicity; hemocompatibility; photoluminescence; wet chemical synthesis