title: Rapid detection of chromosome X, Y, 13, 18, and 21 aneuploidies by primed in situ labeling/synthesis technique.
abstract: OBJECTIVE
  Primed in situ labeling/synthesis (PRINS) technique is an alternative to fluorescent in situ hybridization for chromosome analysis. This study was designed to evaluate the application of PRINS for rapid diagnosis of common chromosomal aneuploidy.
  
  
  METHODS
  We have carried out PRINS using centromere specific oligonucleotide primers for chromosome X, Y, 13, 18 and 21 on lymphocyte metaphase and interphase cells spread. Specific primer was annealed in situ, followed by elongation of primer by Taq DNA polymerase in presence of labeled nucleotides. Finally, reaction was stopped and visualized directly under fluorescent microscope.
  
  
  RESULTS
  Discrete centromere specific signals were observed with each primer.
  
  
  CONCLUSIONS
  PRINS seems to be a rapid and reliable method to detect common chromosome aneuploidy in peripheral blood lymphocyte metaphase and interphase cells.
authors.affiliation: Department of Reproductive Biology, AIIMS, New Delhi, India.
authors.forename: Ashutosh
authors.initials: A
authors.lastname: Halder
authors.forename: Manish
authors.initials: M
authors.lastname: Jain
authors.forename: Isha
authors.initials: I
authors.lastname: Chaudhary
keywords: 13; 18; Aneuploidies; Chromosome X; PRINS; Y; and 21
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