title: Transcription factors lhx1/5-1 and pitx are required for the maintenance and regeneration of serotonergic neurons in planarians.
abstract: In contrast to most adult organisms, freshwater planarians can regenerate any injured body part, including their entire nervous system. This allows for the analysis of genes required for both the maintenance and regeneration of specific neural subtypes. In addition, the loss of specific neural subtypes may uncover previously unknown behavioral roles for that neural population in the context of the adult animal. Here we show that two homeodomain transcription factor homologs, Smed-lhx1/5-1 and Smed-pitx, are required for the maintenance and regeneration of serotonergic neurons in planarians. When either lhx1/5-1 or pitx was knocked down by RNA interference, the expression of multiple canonical markers for serotonergic neurons was lost. Surprisingly, the loss of serotonergic function uncovered a role for these neurons in the coordination of motile cilia on the ventral epidermis of planarians that are required for their nonmuscular gliding locomotion. Finally, we show that in addition to its requirement in serotonergic neurons, Smed-pitx is required for proper midline patterning during regeneration, when it is required for the expression of the midline-organizing molecules Smed-slit in the anterior and Smed-wnt1 in the posterior.
authors.affiliation: Hospital for Sick Children, Program in Developmental and Stem Cell Biology, Toronto, ON, Canada.
authors.forename: Ko W
authors.initials: KW
authors.lastname: Currie
authors.forename: Bret J
authors.initials: BJ
authors.lastname: Pearson
mesh_terms: D000818:Animals; D001483:Base Sequence; D001499:Bayes Theorem; D019295:Computational Biology; D059014:High-Throughput Nucleotide Sequencing; D017403:In Situ Hybridization; D060850:LIM-Homeodomain Proteins / Q000235:genetics / Q000378:metabolism*; D016013:Likelihood Functions; D018715:Microscopy, Video; D008957:Models, Genetic; D008969:Molecular Sequence Data; D051761:Paired Box Transcription Factors / Q000235:genetics / Q000378:metabolism*; D010802:Phylogeny; D010932:Planarians / Q000502:physiology*; D034622:RNA Interference; D012038:Regeneration / Q000502:physiology*; D059326:Serotonergic Neurons / Q000502:physiology*
chemical_list: D060850:LIM-Homeodomain Proteins; D051761:Paired Box Transcription Factors; C101485:homeobox protein PITX1
keywords: Cilia; Locomotion; Lophotrochozoan; Metachronal synchrony; Planarian; Regeneration; Schmidtea mediterranea; Serotonergic neurons; lhx1/5; pitx