title: Introgression of bacterial wilt resistance from eggplant to potato via protoplast fusion and genome components of the hybrids.
abstract: CONCLUSIONS
  Bacterial wilt resistant somatic hybrids were obtained via protoplast fusion between potato and eggplant and three types of nuclear genomes were identified in the hybrids through GISH and SSR analysis.
  Cultivated potato (Solanum tuberosum L.) lacks resistance to bacterial wilt caused by Ralstonia solanacearum. Interspecific symmetric protoplast fusion was conducted to transfer bacterial wilt resistance from eggplant (S. melongena, 2n = 2x = 24) into dihaploid potato (2n = 2x = 24). In total, 34 somatic hybrids were obtained, and of these, 11 rooted and were tested for genome components and resistance to race 1 of R. solanacearum. The hybrids exhibited multiple ploidy levels and contained the dominant nuclear genome from the potato parent. Three types of nuclear genomes were identified in the hybrids through genomic in situ hybridization (GISH) and simple sequence repeat (SSR) analysis, including (1) the potato type of the tetraploids in which eggplant chromosomes could not be detected by GISH but their nuclear DNA was confirmed by SSR, (2) the biased type of the hexaploids in which the chromosome dosage was 2 potato:1 eggplant, and (3) the chromosome translocation type of the mixoploids and aneuploids that was characterized by various rates of translocations of nonhomologous chromosomes. Cytoplasmic genome analysis revealed that mitochondrial DNA of both parents coexisted and/or recombined in most of the hybrids. However, only potato chloroplast DNA was retained in the hybrids speculating a compatibility between cpDNA and nuclear genome of the cell. The pathogen inoculation assay suggested a successful transfer of bacterial wilt resistance from eggplant to the hybrids that provides potential resistance for potato breeding against bacterial wilt. The genome components characterized in present research may explain partially the inheritance behavior of the hybrids which is informative for potato improvement.
authors.affiliation: Key Laboratory of Horticultural Plant Biology (HAU), Ministry of Education, National Centre for Vegetable Improvement (Central China), Huazhong Agricultural University, Wuhan, 430070, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Yu
authors.forename: Wenxuan
authors.initials: W
authors.lastname: Ye
authors.forename: Li
authors.initials: L
authors.lastname: He
authors.forename: Xingkui
authors.initials: X
authors.lastname: Cai
authors.forename: Ting
authors.initials: T
authors.lastname: Liu
authors.forename: Jun
authors.initials: J
authors.lastname: Liu
mesh_terms: D002459:Cell Fusion; D032461:Chromosomes, Plant / Q000235:genetics; D003593:Cytoplasm / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D060467:Disease Resistance / Q000276:immunology*; D005434:Flow Cytometry; D018745:Genome, Plant / Q000235:genetics*; D006824:Hybridization, Genetic; D017403:In Situ Hybridization; D007178:Inbreeding; D007621:Karyotyping; D018895:Microsatellite Repeats / Q000235:genetics; D008938:Mitosis / Q000235:genetics; D010935:Plant Diseases / Q000235:genetics / Q000276:immunology / Q000382:microbiology*; D011003:Ploidies; D011523:Protoplasts / Q000166:cytology*; D043368:Ralstonia solanacearum / Q000502:physiology*; D012038:Regeneration; D032323:Solanum melongena / Q000235:genetics* / Q000276:immunology / Q000382:microbiology; D011198:Solanum tuberosum / Q000235:genetics* / Q000276:immunology / Q000382:microbiology
chemical_list: D018744:DNA, Plant
references.citation: Plant J. 1992 Nov;2(6):863-74
references.pmid: 1302638
references.citation: Plant Sci. 2001 Jan 5;160(2):301-313
references.pmid: 11164602
references.citation: Theor Appl Genet. 1992 Jun;84(1-2):87-91
references.pmid: 24203032
references.citation: Mol Ecol. 1995 Feb;4(1):129-31
references.pmid: 7711952
references.citation: Theor Appl Genet. 1993 Feb;85(6-7):841-50
references.pmid: 24196058
references.citation: Genome. 1994 Oct;37(5):726-35
references.pmid: 18470116
references.citation: Theor Appl Genet. 1996 Jun;92(8):1078-84
references.pmid: 24166639
references.citation: Planta. 2009 Jan;229(2):323-30
references.pmid: 18985381
references.citation: Theor Appl Genet. 1991 Nov;83(1):81-8
references.pmid: 24202260
references.citation: Plant Cell Rep. 2007 Aug;26(8):1263-73
references.pmid: 17443330
references.citation: Biotechnol Adv. 2005 Mar;23(2):131-71
references.pmid: 15694124
references.citation: BMC Plant Biol. 2011 Apr 25;11:71
references.pmid: 21513581
references.citation: Mol Genet Genomics. 2001 May;265(3):387-93
references.pmid: 11405621
references.citation: Plant Sci. 2000 Dec 7;160(1):165-176
references.pmid: 11164589
references.citation: Theor Appl Genet. 2005 Aug;111(3):456-66
references.pmid: 15942755
references.citation: Plant Cell Rep. 2007 Aug;26(8):1233-41
references.pmid: 17406873
references.citation: Theor Appl Genet. 2009 Oct;119(6):1143-53
references.pmid: 19662343
references.citation: Mol Gen Genet. 1998 Aug;259(3):233-45
references.pmid: 9749666
references.citation: Annu Rev Phytopathol. 1991;29:65-87
references.pmid: 18479193
references.citation: Theor Appl Genet. 2002 Jul;105(1):1-7
references.pmid: 12582555
references.citation: Plant Cell Rep. 2010 Nov;29(11):1277-85
references.pmid: 20814793