title: Multiple resistance mechanisms acting in unison in an Escherichia coli clinical isolate.
abstract: The emergence of multiple-resistant isolates poses a serious problem in the hospital environment making it important to evaluate the responsible factors. This work ascertains the mechanisms responsible for the development of resistance in enterobacterial clinical isolates. The major resistance mechanisms have been explored. The presence of target mutations, drug hydrolyzing enzymes, active efflux pump, and drug-resistance genes were elucidated experimentally employing standard methods. One of the clinical isolates was resistant to five classes of structurally unrelated antibiotics and showed involvement of multiple resistance mechanisms. Here, we report the simultaneous presence of multiple drug-resistance mechanisms in an Escherichia coli clinical isolate.
authors.affiliation: Department of Biotechnology, Indian Institute of Technology Kharagpur, Kharagpur, 721302, West Bengal, India.
authors.forename: Dhriti
authors.initials: D
authors.lastname: Mallik
authors.forename: Akash
authors.initials: A
authors.lastname: Kumar
authors.forename: Sujoy Kumar
authors.initials: SK
authors.lastname: Sarkar
authors.forename: Anindya S
authors.initials: AS
authors.lastname: Ghosh
mesh_terms: D000900:Anti-Bacterial Agents / Q000378:metabolism*; D024901:Drug Resistance, Multiple, Bacterial; D004926:Escherichia coli / Q000187:drug effects* / Q000302:isolation & purification; D004927:Escherichia coli Infections / Q000382:microbiology; D006801:Humans; D008826:Microbial Sensitivity Tests
chemical_list: D000900:Anti-Bacterial Agents
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