title: Laboratory selection for increased longevity in Drosophila melanogaster reduces field performance.
abstract: Drosophila melanogaster is frequently used in ageing studies to elucidate which mechanisms determine the onset and progress of senescence. Lines selected for increased longevity have often been shown to perform as well as or superior to control lines in life history, stress resistance and behavioural traits when tested in the laboratory. Functional senescence in longevity selected lines has also been shown to occur at a slower rate. However, it is known that performance in a controlled laboratory setting is not necessarily representative of performance in nature. In this study the effect of ageing, environmental temperature and longevity selection on performance in the field was tested. Flies from longevity selected and control lines of different ages (2, 5, 10 and 15 days) were released in an environment free of natural food sources. Control flies were tested at low, intermediate and high temperatures, while longevity selected flies were tested at the intermediate temperature only. The ability of flies to locate and reach a food source was tested. Flies of intermediate age were generally better at locating resources than both younger and older flies, where hot and cold environments accelerate the senescent decline in performance. Control lines were better able to locate a resource compared to longevity selected lines of the same age, suggesting that longevity comes at a cost in early life field fitness, supporting the antagonistic pleiotropy theory of ageing.
authors.affiliation: Department of Bioscience, Integrative Ecology and Evolution, Aarhus University, Ny Munkegade 114-116, DK-8000 Aarhus C, Denmark. Electronic address: janneke.wit@biology.au.dk.
authors.forename: Janneke
authors.initials: J
authors.lastname: Wit
authors.forename: Torsten Nygaard
authors.initials: TN
authors.lastname: Kristensen
authors.forename: Pernille
authors.initials: P
authors.lastname: Sarup
authors.forename: Jane
authors.initials: J
authors.lastname: Frydenberg
authors.forename: Volker
authors.initials: V
authors.lastname: Loeschcke
mesh_terms: D000222:Adaptation, Physiological; D000375:Aging / Q000235:genetics / Q000502:physiology*; D000818:Animals; D001522:Behavior, Animal; D004331:Drosophila melanogaster / Q000235:genetics / Q000502:physiology*; D004777:Environment; D005260:Female; D056084:Genetic Fitness; D008136:Longevity / Q000235:genetics / Q000502:physiology*; D008297:Male; D008954:Models, Biological; D012641:Selection, Genetic; D013696:Temperature
keywords: Ageing; Environmental temperature; Fitness in the field; Functional senescence; Longevity selection; Release–capture