title: Non-FG mediated transport of the large pre-ribosomal subunit through the nuclear pore complex by the mRNA export factor Gle2.
abstract: Multiple export receptors passage bound pre-ribosomes through nuclear pore complexes (NPCs) by transiently interacting with the Phe-Gly (FG) meshwork of their transport channels. Here, we reveal how the non-FG interacting yeast mRNA export factor Gly-Leu-FG lethal 2 (Gle2) functions in the export of the large pre-ribosomal subunit (pre-60S). Structure-guided studies uncovered conserved platforms used by Gle2 to export pre-60S: an uncharacterized basic patch required to bind pre-60S, and a second surface that makes non-FG contacts with the nucleoporin Nup116. A basic patch mutant of Gle2 is able to function in mRNA export, but not pre-60S export. Thus, Gle2 provides a distinct interaction platform to transport pre-60S to the cytoplasm. Notably, Gle2's interaction platforms become crucial for pre-60S export when FG-interacting receptors are either not recruited to pre-60S or are impaired. We propose that large complex cargos rely on non-FG as well as FG-interactions for their efficient translocation through the nuclear pore complex channel.
authors.affiliation: Department of Biology (D-BIOL), Institute of Biochemistry (IBC), ETH Zurich, Schafmattstrasse 18, CH-8093 Zurich, Switzerland and MLS Program, Life Sciences Zurich Graduate School, Winterthurerstrasse 190, CH-8057 Zurich, Switzerland.
authors.forename: Laura
authors.initials: L
authors.lastname: Occhipinti
authors.forename: Yiming
authors.initials: Y
authors.lastname: Chang
authors.forename: Martin
authors.initials: M
authors.lastname: Altvater
authors.forename: Anna M
authors.initials: AM
authors.lastname: Menet
authors.forename: Stefan
authors.initials: S
authors.lastname: Kemmler
authors.forename: Vikram G
authors.initials: VG
authors.lastname: Panse
mesh_terms: D021581:Active Transport, Cell Nucleus; D000595:Amino Acid Sequence; D001665:Binding Sites; D008969:Molecular Sequence Data; D009154:Mutation; D022022:Nuclear Pore / Q000378:metabolism*; D028861:Nuclear Pore Complex Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D054683:Ribosome Subunits, Large, Eukaryotic / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D016415:Sequence Alignment
chemical_list: C490524:GLE2 protein, S cerevisiae; D028861:Nuclear Pore Complex Proteins; D012333:RNA, Messenger; D029701:Saccharomyces cerevisiae Proteins
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