title: Leaf proteome alterations in the context of physiological and morphological responses to drought and heat stress in barley (Hordeum vulgare L.).
abstract: The objective of this study was to identify barley leaf proteins differentially regulated in response to drought and heat and the combined stresses in context of the morphological and physiological changes that also occur. The Syrian landrace Arta and the Australian cultivar Keel were subjected to drought, high temperature, or a combination of both treatments starting at heading. Changes in the leaf proteome were identified using differential gel electrophoresis and mass spectrometry. The drought treatment caused strong reductions of biomass and yield, while photosynthetic performance and the proteome were not significantly changed. In contrast, the heat treatment and the combination of heat and drought reduced photosynthetic performance and caused changes of the leaf proteome. The proteomic analysis identified 99 protein spots differentially regulated in response to heat treatment, 14 of which were regulated in a genotype-specific manner. Differentially regulated proteins predominantly had functions in photosynthesis, but also in detoxification, energy metabolism, and protein biosynthesis. The analysis indicated that de novo protein biosynthesis, protein quality control mediated by chaperones and proteases, and the use of alternative energy resources, i.e. glycolysis, play important roles in adaptation to heat stress. In addition, genetic variation identified in the proteome, in plant growth and photosynthetic performance in response to drought and heat represent stress adaption mechanisms to be exploited in future crop breeding efforts.
authors.affiliation: Max Planck Institute for Plant Breeding Research, Carl-von-Linné-Weg 10, 50829 Köln, Germany.
authors.forename: J A
authors.initials: JA
authors.lastname: Rollins
authors.forename: E
authors.initials: E
authors.lastname: Habte
authors.forename: S E
authors.initials: SE
authors.lastname: Templer
authors.forename: T
authors.initials: T
authors.lastname: Colby
authors.forename: J
authors.initials: J
authors.lastname: Schmidt
authors.forename: M
authors.initials: M
authors.lastname: von Korff
mesh_terms: D055864:Droughts; D004734:Energy Metabolism / Q000502:physiology; D001467:Hordeum / Q000378:metabolism*; D006358:Hot Temperature; D018515:Plant Leaves / Q000378:metabolism*; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics / Q000379:methods
chemical_list: D020543:Proteome
keywords: Abiotic stress; Rubisco activase; barley; drought; heat; proteomics; yield.
references.citation: Plant Physiol. 1997 Jun;114(2):439-444
references.pmid: 12223718
references.citation: J Exp Bot. 2009;60(12):3531-44
references.pmid: 19561048
references.citation: BMC Genomics. 2006 Sep 08;7:229
references.pmid: 16961921
references.citation: Plant Physiol. 2004 Apr;134(4):1683-96
references.pmid: 15047901
references.citation: Plant Cell. 1989 Aug;1(8):815-25
references.pmid: 2535524
references.citation: J Biol Chem. 1991 Mar 15;266(8):4677-85
references.pmid: 2002016
references.citation: J Exp Bot. 2006;57(1):193-200
references.pmid: 16317034
references.citation: Plant Mol Biol. 1994 Oct;26(1):541-6
references.pmid: 7948905
references.citation: Plant J. 2009 Jul;59(1):14-26
references.pmid: 19309461
references.citation: Planta. 2003 Mar;216(5):736-44
references.pmid: 12624760
references.citation: J Plant Physiol. 2011 Apr 15;168(6):585-93
references.pmid: 21247658
references.citation: Arch Biochem Biophys. 2001 Feb 15;386(2):261-7
references.pmid: 11368350
references.citation: Plant Physiol. 1993 Jan;101(1):209-16
references.pmid: 8278495
references.citation: Plant Sci. 2001 Jan 5;160(2):273-281
references.pmid: 11164599
references.citation: Proteomics. 2012 Sep;12(18):2843-51
references.pmid: 22848051
references.citation: Anal Chem. 2001 Feb 1;73(3):434-8
references.pmid: 11217742
references.citation: J Exp Bot. 2008;59(7):1597-604
references.pmid: 18048372
references.citation: Planta. 2001 Nov;214(1):117-25
references.pmid: 11762161
references.citation: Theor Appl Genet. 2008 Sep;117(5):653-69
references.pmid: 18618094
references.citation: Planta. 1973 Mar;110(1):29-42
references.pmid: 24474309
references.citation: Phytochemistry. 2004 Jun;65(12):1853-63
references.pmid: 15276443
references.citation: Mol Genet Genomics. 2007 Jul;278(1):31-42
references.pmid: 17429694
references.citation: Mol Plant. 2013 Mar;6(2):311-22
references.pmid: 23015761
references.citation: Mol Syst Biol. 2012;8:606
references.pmid: 22929616
references.citation: Planta. 2003 Nov;218(1):1-14
references.pmid: 14513379
references.citation: J Biol Chem. 2003 Aug 29;278(35):32526-36
references.pmid: 12783874
references.citation: Phytochemistry. 2011 Jul;72(10):1180-91
references.pmid: 21257186
references.citation: J Exp Bot. 2009;60(12):3545-57
references.pmid: 19671579
references.citation: Nat Biotechnol. 2011 Mar;29(3):212-4
references.pmid: 21390020
references.citation: Planta. 1999 Jul;209(1):66-76
references.pmid: 10467032
references.citation: Nature. 2012 Nov 29;491(7426):711-6
references.pmid: 23075845
references.citation: Plant Physiol. 2000 Feb;122(2):295-318
references.pmid: 10677424
references.citation: Genetics. 2008 Mar;178(3):1795-805
references.pmid: 18245842
references.citation: Plant Cell Environ. 2006 Aug;29(8):1606-17
references.pmid: 16898021
references.citation: Plant Physiol Biochem. 2013 Feb;63:49-60
references.pmid: 23232247
references.citation: Proteomics. 2011 Dec;11(24):4712-25
references.pmid: 22002954
references.citation: Trends Plant Sci. 2006 Jan;11(1):15-9
references.pmid: 16359910
references.citation: J Exp Bot. 2007;58(2):229-40
references.pmid: 17110587
references.citation: J Exp Bot. 2011 Mar;62(6):1715-29
references.pmid: 21239376
references.citation: Plant Physiol. 2010 Nov;154(3):1254-71
references.pmid: 20807999
references.citation: Nucleic Acids Res. 2010 Jul;38(Web Server issue):W64-70
references.pmid: 20435677
references.citation: Biochimie. 2004 Jul;86(7):439-49
references.pmid: 15308333