title: The effect of surgical wound on ovarian carcinoma growth in an animal model.
abstract: OBJECTIVE
  We examined the effects according to the extent of surgical wound mimicking laparoscopy or laparotomy on ovarian cancer growth in an orthotopic mouse model.
  
  
  METHODS
  To mimic surgery effects, we performed laparoscopy or laparotomy on athymic nude mice under isoflurane inhalation at four days after tumor cell injection. For the laparoscopy model, we performed pneumoperitoneum with CO2, by inserting three cannulars.
  
  
  RESULTS
  Mice in the laparoscopy-mimicking group had significantly lower tumor weight compared to mice in the laparotomy group (p<0.05). In the immediate postoperative period, serum levels of vascular endothelial growth factor (VEGF) and matrix metalloproteinase-2 (MMP2) were significantly lower in the laparoscopy group.
  
  
  CONCLUSIONS
  These results indicate that a minimal surgical wound such as that on laparoscopy, appears to induce little surgical stress on enhancing tumor growth compared to laparotomy in an ovarian cancer animal model, possibly because it minimally influences the secretion of VEGF and MMP2.
authors.affiliation: Department of Obstetrics and Gynecology, Samsung Medical Center, Sungkyunkwan University School of Medicine, 50 Irwon-Dong, Gangnam-gu, Seoul, 135-710, Korea. garden.lee@samsung.com
authors.forename: Jeong-Won
authors.initials: JW
authors.lastname: Lee
authors.forename: Young-Ae
authors.initials: YA
authors.lastname: Park
authors.forename: Young-Jae
authors.initials: YJ
authors.lastname: Cho
authors.forename: Kyu-Ho
authors.initials: KH
authors.lastname: Kang
authors.forename: Jung-Joo
authors.initials: JJ
authors.lastname: Choi
authors.forename: Yoo-Young
authors.initials: YY
authors.lastname: Lee
authors.forename: Tae-Joong
authors.initials: TJ
authors.lastname: Kim
authors.forename: Chel Hun
authors.initials: CH
authors.lastname: Choi
authors.forename: Byoung-Gie
authors.initials: BG
authors.lastname: Kim
authors.forename: Duk-Soo
authors.initials: DS
authors.lastname: Bae
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D004195:Disease Models, Animal; D005260:Female; D010535:Laparoscopy; D007813:Laparotomy; D020778:Matrix Metalloproteinase 2 / Q000097:blood; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D051379:Mice; D009389:Neovascularization, Pathologic / Q000473:pathology; D010051:Ovarian Neoplasms / Q000097:blood / Q000201:enzymology / Q000473:pathology* / Q000601:surgery*; D047368:Tumor Burden; D042461:Vascular Endothelial Growth Factor A / Q000097:blood; D014945:Wound Healing
chemical_list: D042461:Vascular Endothelial Growth Factor A; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9
keywords: Surgical stress; laparoscopy; mouse model; ovarian carcinoma