title: Protective activity of ischemic preconditioning on rat testicular ischemia: effects of Y-27632 and 5-hydroxydecanoic acid.
abstract: OBJECTIVE
  The aim of this study was to investigate the role of ischemic preconditioning (IPC) on ischemia/reperfusion (I/R)-induced injury of rat testis and determine the effects of 5-hydroxydecanoic acid (5-HD), a selective K(ATP) channel antagonist, and Y-27632, a selective Rho kinase inhibitor, on IPC.
  
  
  METHODS
  I/R injury was induced by 180 min ischemia and 60 min reperfusion of testis. There were 5 groups. Group 1 served as untreated controls. The rats in Group 2 were subjected to I/R only. In Group 3, 3 cycles of IPC (5 min transient ischemia plus 5 min reperfusion) were performed prior to I/R. In groups 4 and 5, the rats were treated as in Group 3 but received intraperitoneal injections of 0.3 mg/kg Y-27632 or 10 mg/kg 5-HD prior to IPC, respectively.
  
  
  RESULTS
  I/R led to severe histopathological lesions in the rat testis and significantly lowered the scoring. I/R resulted in significant elevation in tissue lipid peroxide levels, myeloperoxidase (MPO) activity, and total antioxidative capacity (TAC), total oxidative status, and oxidative stress index levels. Protective effects of IPC on I/R-induced testicular injury of rats were observed with the significant recovery in these biochemical parameters. Y-27632 treatment led to a significant decrease in MPO activity, but there were no significant changes in the remaining parameters. Effects of IPC were blocked by 5-HD except in the TAC levels.
  
  
  CONCLUSIONS
  Our results showed that IPC protected rat testis against I/R-induced injury via activation of KATP channels. Additionally, Rho kinase inhibition preserved the effects of IPC in testis.
authors.affiliation: Department of Pediatric Surgery, Faculty of Medicine, University of Gaziantep, Gaziantep, 27310, Turkey.
authors.forename: Ahmet
authors.initials: A
authors.lastname: Gozen
authors.forename: Seniz
authors.initials: S
authors.lastname: Demiryurek
authors.forename: Abdullah
authors.initials: A
authors.lastname: Taskin
authors.forename: Harun
authors.initials: H
authors.lastname: Ciralik
authors.forename: Hasan
authors.initials: H
authors.lastname: Bilinc
authors.forename: Sevgül
authors.initials: S
authors.lastname: Kara
authors.forename: Abdullah
authors.initials: A
authors.lastname: Aydin
authors.forename: Nurten
authors.initials: N
authors.lastname: Aksoy
authors.forename: Haluk
authors.initials: H
authors.lastname: Ceylan
mesh_terms: D000577:Amides / Q000494:pharmacology*; D000818:Animals; D003652:Decanoic Acids / Q000494:pharmacology*; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D006880:Hydroxy Acids / Q000494:pharmacology*; D007511:Ischemia / Q000517:prevention & control*; D019194:Ischemic Preconditioning; D008297:Male; D011725:Pyridines / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D013737:Testis / Q000098:blood supply*
chemical_list: D000577:Amides; D003652:Decanoic Acids; D004791:Enzyme Inhibitors; D006880:Hydroxy Acids; D011725:Pyridines; C108830:Y 27632; C052853:5-hydroxydecanoic acid
keywords: 5-Hydroxydecanoic acid; Ischemic preconditioning; Rho kinase; Testis; Y-27632