title: Diagnosis of pathological minor salivary glands in primary Sjogren's syndrome by using Raman spectroscopy.
abstract: The lip biopsy is essential for the diagnosis of primary Sjogren's syndrome (SS) but an invasive method can cause some disadvantages. The purpose of this study is to apply Raman spectroscopy to detect the pathological minor salivary glands in primary SS and establish the diagnostic model of Raman spectra of the primary SS samples. Raman spectra from the primary samples and control samples were obtained by Raman microscope and were compared to find the differences. The principal component analysis (PCA) and discrimination function analysis (DFA) were employed to analyze the spectra and establish the diagnostic model. The differences of Raman spectra demonstrated the biochemical molecular alterations between the different samples. Compared with the control samples, the content of proteins, nucleic acids, and keratin increased in the primary SS samples but the content of lipids decreased. PCA and DFA displayed a powerful role in the classification of the Raman spectra. The sensitivity and specificity of the diagnostic model reached above 91 and 92%, respectively. The total accuracy is 92.4%. Raman spectroscopy combined with PCA-DFA algorithm will provide an effective and accurate technology for the diagnosis of the pathological minor salivary glands in primary SS, which may replace the lip biopsy in the future.
authors.affiliation: Department of Stomatology, the First Affiliated Hospital of Xiamen University, Xiamen, China.
authors.forename: Lili
authors.initials: L
authors.lastname: Xue
authors.forename: Pei
authors.initials: P
authors.lastname: Sun
authors.forename: Dongchen
authors.initials: D
authors.lastname: Ou
authors.forename: Peiqiong
authors.initials: P
authors.lastname: Chen
authors.forename: Meiqing
authors.initials: M
authors.lastname: Chen
authors.forename: Bing
authors.initials: B
authors.lastname: Yan
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001706:Biopsy; D006801:Humans; D008048:Lip Neoplasms / Q000473:pathology; D008297:Male; D008875:Middle Aged; D025341:Principal Component Analysis; D012470:Salivary Glands, Minor / Q000473:pathology*; D012680:Sensitivity and Specificity; D012859:Sjogren's Syndrome / Q000175:diagnosis / Q000473:pathology*; D013059:Spectrum Analysis, Raman / Q000379:methods*; D055815:Young Adult
references.citation: Joint Bone Spine. 2009 Jan;76(1):44-9
references.pmid: 18829369
references.citation: Exp Mol Pathol. 2007 Apr;82(2):121-9
references.pmid: 17320864
references.citation: J Invest Dermatol. 2002 Jul;119(1):64-9
references.pmid: 12164926
references.citation: Med Hypotheses. 2011 Feb;76(2):176-7
references.pmid: 20934259
references.citation: Oral Oncol. 2011 May;47(5):430-5
references.pmid: 21439894
references.citation: Biochim Biophys Acta. 2007 Oct;1768(10):2605-15
references.pmid: 17761139
references.citation: Arch Med Res. 2002 Mar-Apr;33(2):95-106
references.pmid: 11886706
references.citation: Laryngoscope. 2007 Jun;117(6):1004-8
references.pmid: 17417107
references.citation: Appl Spectrosc. 2009 Oct;63(10):1089-94
references.pmid: 19843357
references.citation: Anal Bioanal Chem. 2007 Mar;387(5):1649-56
references.pmid: 17043798
references.citation: Appl Spectrosc. 2003 Sep;57(9):1100-16
references.pmid: 14611040
references.citation: Lasers Med Sci. 2006 Dec;21(4):192-7
references.pmid: 17024320
references.citation: Biosens Bioelectron. 2011 Mar 15;26(7):3167-74
references.pmid: 21227679
references.citation: Best Pract Res Clin Rheumatol. 2007 Dec;21(6):989-1010
references.pmid: 18068857
references.citation: J Biomed Opt. 2005 Jul-Aug;10(4):44007
references.pmid: 16178641
references.citation: Arch Med Res. 2006 Nov;37(8):921-32
references.pmid: 17045106
references.citation: Biophys Chem. 2009 Mar;140(1-3):57-61
references.pmid: 19070416
references.citation: Autoimmun Rev. 2010 Mar;9(5):A305-10
references.pmid: 19903539
references.citation: Biopolymers. 2006 Feb 15;81(3):179-93
references.pmid: 16231284
references.citation: Best Pract Res Clin Rheumatol. 2004 Jun;18(3):313-29
references.pmid: 15158743
references.citation: Lasers Med Sci. 2012 Mar;27(2):487-95
references.pmid: 21837504