title: Performance of MALDI-ToF MS for species identification of Burkholderia cepacia complex clinical isolates.
abstract: We evaluated the performance of matrix-assisted laser desorption ionization-time of flight (MALDI-ToF) for identification of Bcc species compared with that of recA sequencing. MALDI-ToF was able of identifying 100% of Bcc isolates at the genus level, but 23.1% of Bcc isolates tested were not correctly identified at the species level. The misidentification occurred most frequently with Burkholderia contaminans (100%) and B. cepacia (33.3%).
authors.affiliation: Departamento de Medicina, Laboratório Alerta, Universidade Federal de São Paulo, São Paulo, Brazil. Electronic address: lorenafehlberg@gmail.com.
authors.forename: Lorena Cristina Corrêa
authors.initials: LC
authors.lastname: Fehlberg
authors.forename: Lucas Henrique Sales
authors.initials: LH
authors.lastname: Andrade
authors.forename: Diego Magno
authors.initials: DM
authors.lastname: Assis
authors.forename: Rosana Helena Vicente
authors.initials: RH
authors.lastname: Pereira
authors.forename: Ana Cristina
authors.initials: AC
authors.lastname: Gales
authors.forename: Elizabeth Andrade
authors.initials: EA
authors.lastname: Marques
mesh_terms: D015373:Bacterial Typing Techniques / Q000379:methods*; D019121:Burkholderia Infections / Q000382:microbiology; D042602:Burkholderia cepacia complex / Q000737:chemistry / Q000145:classification* / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000235:genetics; D006801:Humans; D016133:Polymerase Chain Reaction; D011938:Rec A Recombinases / Q000235:genetics; D017422:Sequence Analysis, DNA; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
chemical_list: D004269:DNA, Bacterial; D011938:Rec A Recombinases
keywords: Bacteria identification; Bruker; Mass spectrometry; Non-fermentative bacilli; recA gene sequencing