title: Expression profile of oestrogen receptors and oestrogen-related receptors is organ specific and sex dependent: the Japanese medaka Oryzias latipes model.
abstract: Gene expression of all known subtypes of oestrogen receptor (ER) and oestrogen-related receptor (ERR) in multiple organs and both sexes of the Japanese medaka Oryzias latipes was profiled and systematically analysed. As revealed by statistical analyses and low-dimensional projections, the expressions of ERRs proved to be organ and sex dependent, which is in contrast with the ubiquitous nature of ERs. Moreover, expressions of specific ERR isoforms (ERRγ1, ERRγ2) were strongly correlated with that of all ERs (ERα, ERβ1 and ERβ2), suggesting the existence of potential interactions. Findings of this study shed light on the co-regulatory role of particular ERRs in oestrogen-ERs signalling and highlight the potential importance of ERRs in determining organ and sex-specific oestrogen responses. Using O. latipes as an alternative vertebrate model, this study provides new directions that call for collective efforts from the scientific community to unravel the mechanistic action of ER-ERR cross-talks, and their intertwining functions, in a cell and sex-specific manner in vivo.
authors.affiliation: Department of Biology and Chemistry, City University of Hong Kong, 83 Tat Chee Avenue, Kowloon, Hong Kong SAR.
authors.forename: N K M
authors.initials: NK
authors.lastname: Cheung
authors.forename: A C K
authors.initials: AC
authors.lastname: Cheung
authors.forename: R R
authors.initials: RR
authors.lastname: Ye
authors.forename: W
authors.initials: W
authors.lastname: Ge
authors.forename: J P
authors.initials: JP
authors.lastname: Giesy
authors.forename: D W T
authors.initials: DW
authors.lastname: Au
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism; D005260:Female; D020869:Gene Expression Profiling; D005880:Gills / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008297:Male; D009206:Myocardium / Q000378:metabolism; D009990:Oryzias / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D010053:Ovary / Q000378:metabolism; D010802:Phylogeny; D025341:Principal Component Analysis; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D011960:Receptors, Estrogen / Q000235:genetics / Q000378:metabolism*; D012727:Sex Characteristics; D013154:Spleen / Q000378:metabolism; D013737:Testis / Q000378:metabolism
chemical_list: D020033:Protein Isoforms; D011960:Receptors, Estrogen
keywords: oestrogen regulation; oestrogenic effects; organs; teleost; vertebrate