title: Expression patterns of peroxisome proliferator-activated receptor gamma 1 versus gamma 2, and their association with intramuscular fat in goat tissues.
abstract: Intramuscular fat (IMF) shortage causes the lack of juiciness and tenderness of goat meat, while peroxisome proliferator-activated receptor gamma 1 (PPARγ1) and gamma 2 (PPARγ2) play key roles in lipid metabolism. Nevertheless, their expression patterns and the relationship with IMF have been poorly exposed. Using quantitative polymerase chain reaction (qPCR), classical Soxhlet extraction, and in situ hybridization, we demonstrated that among 13 goat tissues, expression of PPARγ1 was dramatically higher than that of PPARγ2 except for lung. We further demonstrated the expression patterns of PPARγ1 and PPARγ2 and their negative association with intramuscular fat content in three goat muscles with kids growing. Meanwhile, PPARγ expression was located in the connective tissues. These results suggest that PPARγ1 is rather active for most tissues of goat, and closely related with the muscular fat metabolism during early postnatal life, but a more direct proof remains to be provided.
authors.affiliation: College of Animal Science and Technology, Sichuan Agricultural University, Ya'an 625014, China.
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.forename: Jing
authors.initials: J
authors.lastname: Jiang
authors.forename: Linjie
authors.initials: L
authors.lastname: Wang
authors.forename: Tao
authors.initials: T
authors.lastname: Zhong
authors.forename: Bo
authors.initials: B
authors.lastname: Chen
authors.forename: Siyuan
authors.initials: S
authors.lastname: Zhan
authors.forename: Hongping
authors.initials: H
authors.lastname: Zhang
authors.forename: Lixin
authors.initials: L
authors.lastname: Du
mesh_terms: D000273:Adipose Tissue / Q000033:anatomy & histology / Q000378:metabolism*; D050154:Adiposity; D000818:Animals; D005260:Female; D015870:Gene Expression; D006041:Goats / Q000033:anatomy & histology / Q000235:genetics* / Q000378:metabolism; D008297:Male; D018482:Muscle, Skeletal / Q000033:anatomy & histology / Q000378:metabolism*; D009928:Organ Specificity; D047495:PPAR gamma / Q000235:genetics* / Q000378:metabolism; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
chemical_list: D047495:PPAR gamma; D020033:Protein Isoforms; D012333:RNA, Messenger
keywords: Expression; Goat; IMF; In situ hybridization; Intramuscular fat; LD; PPARγ; PPARγ1; PPARγ2; Peroxisome proliferator-activated receptor gamma; SM; Skeletal muscle; TB; intramuscular fat; m. longissimus dorsi; m. semimembranosus; m. triceps brachii; peroxisome proliferator-activated receptor gamma; peroxisome proliferator-activated receptor gamma 1; peroxisome proliferator-activated receptor gamma 2; qPCR; quantitative polymerase chain reaction