title: Influence of mineral, olive or sunflower oils on male reproductive parameters in vitro--the wild rodent Calomys laucha.
abstract: The aim of this study was to evaluate the influence of oils on male reproductive parameters in Calomys laucha. Twenty-four animals were distributed into four groups and given the following substances by gavage: water, mineral oil, olive oil and sunflower oil. After 10 days of gavage, the animals were euthanised and the semen was collected from them for assessing acrosome integrity and carrying out in vitro penetration (IVP) test. Acrosome was significantly reduced (P < 0.05) for the vehicles in relation to control. In vitro penetration was reduced in all vehicles in relation to control, but only sunflower oil had statistically lower levels of reduction (P < 0.05). Oily vehicles are able to influence in vitro reproductive tests negatively, interfering in reproductive toxicological studies.
authors.affiliation: ReproPel, Faculdade de Veterinária, Campus Capão do Leão, Universidade Federal de Pelotas (UFPel), Pelotas, RS, Brazil.
authors.forename: T F
authors.initials: TF
authors.lastname: Cardoso
authors.forename: A S
authors.initials: AS
authors.lastname: Varela
authors.forename: E F
authors.initials: EF
authors.lastname: Silva
authors.forename: J
authors.initials: J
authors.lastname: Vilela
authors.forename: A
authors.initials: A
authors.lastname: Hartmann
authors.forename: R D
authors.initials: RD
authors.lastname: Jardim
authors.forename: E P
authors.initials: EP
authors.lastname: Colares
authors.forename: C D
authors.initials: CD
authors.lastname: Corcini
mesh_terms: D000818:Animals; D066298:In Vitro Techniques; D008297:Male; D008899:Mineral Oil / Q000494:pharmacology*; D000069463:Olive Oil; D010938:Plant Oils / Q000494:pharmacology*; D012098:Reproduction / Q000187:drug effects*; D012377:Rodentia / Q000502:physiology*; D013094:Spermatozoa / Q000187:drug effects; D000074242:Sunflower Oil
chemical_list: D000069463:Olive Oil; D010938:Plant Oils; D000074242:Sunflower Oil; D008899:Mineral Oil
keywords: Acrosome; heterologous penetration; sperm maturation; toxicology