title: [HPLC-MS/MS for determination of astragalin in rat plasma and its pharmacokinetics].
abstract: OBJECTIVE
  To develop a LC-MS/MS method for determination of astragalin in rat plasma and study its pharmacokinetics in rats.
  
  
  METHODS
  The analytical column was packed with ZORBAX SB-C18, and the mobile phase was methanol-water containing 10 mmol/L ammonium acetate-formic acid (80:20:0.15, V/V/V). Quercetin was used as the internal standard (IS). Multiple reaction monitoring (MRM) mode was employed, and the transition of m/z was m/z 449.1→m/z 287.1 for astragalin, and m/z 301.1→m/z 151.1 for IS.
  
  
  RESULTS
  A good liner relationship was obtained within the range of 1.00-1000 ng/ml (r(2)=0.9929), and the lower limit of quantification of astragalin was 1.00 ng/ml in rat plasma. The extraction recoveries were all above 93%. After oral administration of astragalin, the maximum plasma concentration of astragalin was 231.1∓67.3 ng/ml and the time to reach this value was 0.5∓0.1 h, with a half-life of 3.9∓1.3 h and an AUC of 782.6∓152.8 ng·h/ml.
  
  
  CONCLUSIONS
  The method is highly sensitive, selective and rapid for determination of the concentration of astragalin in rat plasma to facilitates the study of its pharmacokinetics.
authors.affiliation: School of Pharmacy, Guangdong Medical College, Dongguan, China. Lhju88@126.com
authors.forename: Hongju
authors.initials: H
authors.lastname: Liu
authors.forename: Chong
authors.initials: C
authors.lastname: Yan
authors.forename: Baohong
authors.initials: B
authors.lastname: Li
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D044949:Kaempferols / Q000097:blood* / Q000493:pharmacokinetics*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D053719:Tandem Mass Spectrometry
chemical_list: D044949:Kaempferols; C001579:astragalin