title: Role of fibroblast growth factor 8 in neurite outgrowth from spiral ganglion neurons in vitro.
abstract: Many neurons degenerate after injuries resulting from overstimulation, drugs, genetic mutations, and aging. Although several growth factors and neurotrophins delay degeneration and promote regrowth of neural processes, the role of fibroblast growth factor 8 (FGF8) in mammalian spiral ganglion neurons (SGN) neurite outgrowth has not been examined. This study develops and uses SGN cell cultures suitable for experimental analysis, it investigates whether FGF8a and FGF8b isoforms affect the neurite outgrowth from SGN cultured in vitro. We found that both FGF8a and FGF8b promoted the outgrowth of neurites from cultured SGN. This response is mediated by FGF receptors and involves the activation of IκBα-mediated NFκB signaling pathway. These findings suggest that, besides its morphogenetic role during development, FGF8 may have trophic functions in the adult which are relevant to regeneration.
authors.affiliation: Department of Neuroscience, University of Connecticut Health Center, Farmington, Connecticut 06030, USA. sghernandez@uchc.edu
authors.forename: Sofía
authors.initials: S
authors.lastname: García-Hernández
authors.forename: Steven J
authors.initials: SJ
authors.lastname: Potashner
authors.forename: D Kent
authors.initials: DK
authors.lastname: Morest
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D000831:Animals, Newborn; D002478:Cells, Cultured; D000089622:Doublecortin Domain Proteins; D004347:Drug Interactions; D004791:Enzyme Inhibitors / Q000494:pharmacology; D051524:Fibroblast Growth Factor 8 / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D051379:Mice; D008813:Mice, Inbred ICR; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D016501:Neurites / Q000187:drug effects*; D009474:Neurons / Q000166:cytology*; D009479:Neuropeptides / Q000378:metabolism; D020033:Protein Isoforms / Q000494:pharmacology; D011743:Pyrimidines / Q000494:pharmacology; D013136:Spiral Ganglion / Q000166:cytology*; D014404:Tubulin / Q000378:metabolism
chemical_list: D000089622:Doublecortin Domain Proteins; D004791:Enzyme Inhibitors; D008869:Microtubule-Associated Proteins; D009479:Neuropeptides; C115711:PD 173074; D020033:Protein Isoforms; D011743:Pyrimidines; D014404:Tubulin; C462941:beta3 tubulin, mouse; D051524:Fibroblast Growth Factor 8
keywords: Development; FGF8 isoforms; Morphogen; Regeneration; Trophic function
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