title: [HIV-1 infection changes miRNA expression profile in the whole blood].
abstract: To describe the unique miRNA profiles for HIV seropositive individuals and identify significantly differently expressed miRNAs, we determined the expression level of 754 miRNAs of 10 HIV seropositive individuals and 10 HIV seronegative individuals by using the Taqman low density microRNA array. BRB-Array Tool was used to conduct the significance analysis, and the DIANA online tool was used to perform the miRNA target prediction and pathway analysis. A total of 56 significantly differentially expressed miRNAs were identified by microarray between HIV seropositive and seronegative individuals. Among them, 49 miRNAs were down-regulated and 7 were up-regulated, partially overlapped with reported data. Predicted target genes were mainly involved in MAPK, TGF-beta and Wnt pathways. The results shows that miRNA profile changes in HIV-1 seropositive individuals, and the 56 differentially expressed miRNAs may play important role during HIV infection. Further studies on these miRNAs may be helpful for identify key molecules involved in HIV infection and potential diagnostic markers.
authors.affiliation: Wenzhou Medical Colleague, Wenzhou 325035,China.
authors.forename: Ling-Yan
authors.initials: LY
authors.lastname: Zhu
authors.forename: Chao
authors.initials: C
authors.lastname: Qiu
authors.forename: Jian-Xin
authors.initials: JX
authors.lastname: Lv
authors.forename: Jian-Qing
authors.initials: JQ
authors.lastname: Xu
mesh_terms: D000328:Adult; D005260:Female; D020869:Gene Expression Profiling; D015658:HIV Infections / Q000097:blood / Q000235:genetics* / Q000821:virology; D015497:HIV-1 / Q000235:genetics / Q000502:physiology*; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000097:blood / Q000235:genetics*; D055815:Young Adult
chemical_list: D035683:MicroRNAs