title: Transgenic sweet orange plants expressing a dermaseptin coding sequence show reduced symptoms of citrus canker disease.
abstract: Citrus canker provoked by Xanthomonas axonopodis pv. citri is a bacterial disease causing severe losses in all citrus-producing areas around the world. Xanthomonas infection is considered as an endemic disease in Northeast and Northwest Argentina, affecting as much as 10% of commercial citrus plantations. There is not known natural resistance neither in orange varieties nor in rootstocks used for grafting of commercial cultivars. To introduce resistance to this disease, plants of Pineapple sweet orange were transformed with a genetic construct allowing constitutive accumulation of dermaseptin. In comparison with non-transformed plants, transgenic plants showed symptom reduction levels of up to 50% in in planta assays performed under controlled conditions.
authors.affiliation: Laboratorio de Agrobiotecnología, Departamento de Fisiología Molecular y Celular, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Intendente Güiraldes 2160, Ciudad Universitaria, C1428EGA Buenos Aires, Argentina.
authors.forename: Nicolás
authors.initials: N
authors.lastname: Furman
authors.forename: Ken
authors.initials: K
authors.lastname: Kobayashi
authors.forename: Maria Cecilia
authors.initials: MC
authors.lastname: Zanek
authors.forename: Javier
authors.initials: J
authors.lastname: Calcagno
authors.forename: Maria Laura
authors.initials: ML
authors.lastname: Garcia
authors.forename: Alejandro
authors.initials: A
authors.lastname: Mentaberry
mesh_terms: D016960:Agrobacterium tumefaciens; D029845:Amphibian Proteins / Q000235:genetics* / Q000378:metabolism* / Q000494:pharmacology; D000900:Anti-Bacterial Agents / Q000378:metabolism / Q000494:pharmacology; D023181:Antimicrobial Cationic Peptides / Q000235:genetics* / Q000378:metabolism* / Q000494:pharmacology; D032084:Citrus sinensis / Q000235:genetics* / Q000382:microbiology*; D024881:Drug Resistance, Bacterial; D018506:Gene Expression Regulation, Plant; D016366:Open Reading Frames; D010935:Plant Diseases / Q000382:microbiology* / Q000517:prevention & control*; D018515:Plant Leaves / Q000382:microbiology; D030821:Plants, Genetically Modified; D014170:Transformation, Genetic; D014974:Xanthomonas / Q000187:drug effects*
chemical_list: D029845:Amphibian Proteins; D000900:Anti-Bacterial Agents; D023181:Antimicrobial Cationic Peptides; C070905:dermaseptin
keywords: Antibacterial resistance; Canker disease; Dermaseptin; Transgenic orange