title: [Effect evaluation of three ELISA kits in detection of fasciolasis].
abstract: OBJECTIVE
  To evaluate the effect of 3 ELISA kits on detection of human fasciolasis.
  
  
  METHODS
  Twenty-six serum samples from patients with fasciolasis, 180 serum samples from patients with other parasitic diseases as well as 26 serum samples from healthy people were detected by ELISA kits which using soluble antigen of Fasciola gigantica, Fasciola hepatica (Fg-ELISA and Fh-ELISA) as well as IgG antigen ELISA detection kits made by DRG company in Germany. The effects of the 3 kits were evaluated.
  
  
  RESULTS
  The sensitivities of Fg-ELISA, Fh-ELISA, and DRG-ELISA were 100.0%, 80.8% (95% CI: 65.7%-95.9%) and 100.0%, respectively; the specificities of the three were 87.9% (95% CI: 83.5%-92.4%), 85.0%(95% CI: 80.1%-89.9%) and 83.5% (95% CI: 78.4%-88.6%), respectively, and Youden indexes of them were 0.88, 0.66 and 0.84, respectively. The detection rate of Fg-ELISA (100%) was significantly higher than that of Fh-ELISA (80.8%) (P < 0.05). The A absolute value (A/CO) of Fg-ELISA was 1.70, which was also significantly higher than the value of Fh-ELISA (1.18) (P < 0.000 1).
  
  
  CONCLUSIONS
  Fg-ELISA has a good detection effect and low cost, and is more suitable than Fh-ELISA and DRG-ELISA for clinical sample tests as well as massive screening in fasciolasis endemic areas in southwest China.
authors.affiliation: National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, WHO Collaborating Center of Malaria, Schistosomiasis and Filariasis, Shanghai 200025, China
authors.forename: Lin
authors.initials: L
authors.lastname: Ai
authors.forename: Mu-Xin
authors.initials: MX
authors.lastname: Chen
authors.forename: Shao-Hong
authors.initials: SH
authors.lastname: Chen
authors.forename: Yan-Hong
authors.initials: YH
authors.lastname: Chu
authors.forename: Yu-Chun
authors.initials: YC
authors.lastname: Cai
authors.forename: Xiao-Nong
authors.initials: XN
authors.lastname: Zhou
authors.forename: Jia-Xu
authors.initials: JX
authors.lastname: Chen
mesh_terms: D000818:Animals; D000947:Antigens, Helminth / Q000032:analysis / Q000276:immunology; D002681:China; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005209:Fasciola / Q000276:immunology; D005211:Fascioliasis / Q000175:diagnosis* / Q000276:immunology; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology
chemical_list: D000947:Antigens, Helminth; D007074:Immunoglobulin G