title: Low-temperature time-resolved spectroscopic study of the major light-harvesting complex of Amphidinium carterae.
abstract: The major light-harvesting complex of Amphidinium (A.) carterae, chlorophyll-a-chlorophyll-c 2-peridinin-protein complex (acpPC), was studied using ultrafast pump-probe spectroscopy at low temperature (60 K). An efficient peridinin-chlorophyll-a energy transfer was observed. The stimulated emission signal monitored in the near-infrared spectral region was stronger when redder part of peridinin pool was excited, indicating that these peridinins have the S1/ICT (intramolecular charge-transfer) state with significant charge-transfer character. This may lead to enhanced energy transfer efficiency from "red" peridinins to chlorophyll-a. Contrary to the water-soluble antenna of A. carterae, peridinin-chlorophyll-a protein, the energy transfer rates in acpPC were slower under low-temperature conditions. This fact underscores the influence of the protein environment on the excited-state dynamics of pigments and/or the specificity of organization of the two pigment-protein complexes.
authors.affiliation: Faculty of Science, University of South Bohemia, Branišovská 31, 370 05, České Budějovice, Czech Republic.
authors.forename: Václav
authors.initials: V
authors.lastname: Slouf
authors.forename: Marcel
authors.initials: M
authors.lastname: Fuciman
authors.forename: Silke
authors.initials: S
authors.lastname: Johanning
authors.forename: Eckhard
authors.initials: E
authors.lastname: Hofmann
authors.forename: Harry A
authors.initials: HA
authors.lastname: Frank
authors.forename: Tomáš
authors.initials: T
authors.lastname: Polívka
mesh_terms: D002338:Carotenoids / Q000378:metabolism; D002734:Chlorophyll / Q000378:metabolism; D000077194:Chlorophyll A; D003080:Cold Temperature; D004141:Dinoflagellida / Q000378:metabolism*; D004583:Electrons; D004735:Energy Transfer; D007700:Kinetics; D045342:Light-Harvesting Protein Complexes / Q000378:metabolism*; D019265:Spectroscopy, Near-Infrared; D013997:Time Factors
chemical_list: D045342:Light-Harvesting Protein Complexes; D002734:Chlorophyll; C016040:peridinin; D002338:Carotenoids; D000077194:Chlorophyll A
references.citation: Photosynth Res. 2014 May;120(1-2):125-39
references.pmid: 23361658
references.citation: Biochim Biophys Acta. 2010 Sep;1797(9):1647-56
references.pmid: 20460100
references.citation: J Phys Chem B. 2006 Jan 12;110(1):512-21
references.pmid: 16471563
references.citation: J Phys Chem A. 2012 Dec 20;116(50):12330-8
references.pmid: 23176366
references.citation: Proc Natl Acad Sci U S A. 2009 Dec 8;106(49):20764-9
references.pmid: 19934052
references.citation: Biochemistry. 2006 Jul 18;45(28):8516-26
references.pmid: 16834325
references.citation: Biochemistry. 2006 Nov 28;45(47):14052-63
references.pmid: 17115700
references.citation: Science. 2004 Jul 16;305(5682):354-60
references.pmid: 15256663
references.citation: Biochim Biophys Acta. 2012 Jul;1817(7):983-9
references.pmid: 22497797
references.citation: Biochim Biophys Acta. 2010 May;1797(5):543-9
references.pmid: 20117075
references.citation: Biochemistry. 2003 Nov 11;42(44):13027-34
references.pmid: 14596618
references.citation: Photosynth Res. 2005 Nov;86(1-2):241-50
references.pmid: 16172942
references.citation: J Eukaryot Microbiol. 2003 Nov-Dec;50(6):439-48
references.pmid: 14733436
references.citation: Biochim Biophys Acta. 2010 Oct;1797(10):1759-67
references.pmid: 20599677
references.citation: Arch Biochem Biophys. 2007 Feb 15;458(2):111-20
references.pmid: 17098207
references.citation: Biochim Biophys Acta. 2009 Oct;1787(10):1189-97
references.pmid: 19486881
references.citation: Acc Chem Res. 2010 Aug 17;43(8):1125-34
references.pmid: 20446691
references.citation: Photochem Photobiol. 1993 Jan;57(1):125-31
references.pmid: 8502722
references.citation: Biophys J. 2013 Mar 19;104(6):1314-25
references.pmid: 23528091
references.citation: Biochem Biophys Res Commun. 2012 Oct 26;427(3):637-41
references.pmid: 23026044
references.citation: Phys Chem Chem Phys. 2011 Jun 14;13(22):10762-70
references.pmid: 21552626
references.citation: ISME J. 2007 Aug;1(4):271-82
references.pmid: 18043638
references.citation: Chem Phys. 2010 Jul 19;373(1-2):80-89
references.pmid: 20689726
references.citation: Proc Natl Acad Sci U S A. 2002 Dec 24;99(26):16760-5
references.pmid: 12486228
references.citation: Science. 1998 Jul 10;281(5374):237-40
references.pmid: 9657713
references.citation: PLoS One. 2012;7(10):e47456
references.pmid: 23112815
references.citation: Science. 1996 Jun 21;272(5269):1788-91
references.pmid: 8650577
references.citation: Plant Mol Biol. 1994 Feb;24(4):673-7
references.pmid: 8155886
references.citation: J Phys Chem A. 2005 Apr 14;109(14):3120-7
references.pmid: 16833638
references.citation: Photosynth Res. 2005 Nov;86(1-2):217-27
references.pmid: 16172940