title: Polo kinase Cdc5 is a central regulator of meiosis I.
abstract: During meiosis, two consecutive rounds of chromosome segregation yield four haploid gametes from one diploid cell. The Polo kinase Cdc5 is required for meiotic progression, but how Cdc5 coordinates multiple cell-cycle events during meiosis I is not understood. Here we show that CDC5-dependent phosphorylation of Rec8, a subunit of the cohesin complex that links sister chromatids, is required for efficient cohesin removal from chromosome arms, which is a prerequisite for meiosis I chromosome segregation. CDC5 also establishes conditions for centromeric cohesin removal during meiosis II by promoting the degradation of Spo13, a protein that protects centromeric cohesin during meiosis I. Despite CDC5's central role in meiosis I, the protein kinase is dispensable during meiosis II and does not even phosphorylate its meiosis I targets during the second meiotic division. We conclude that Cdc5 has evolved into a master regulator of the unique meiosis I chromosome segregation pattern.
authors.affiliation: Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
authors.forename: Michelle A
authors.initials: MA
authors.lastname: Attner
authors.forename: Matthew P
authors.initials: MP
authors.lastname: Miller
authors.forename: Ly-sha
authors.initials: LS
authors.lastname: Ee
authors.forename: Sheryl K
authors.initials: SK
authors.lastname: Elkin
authors.forename: Angelika
authors.initials: A
authors.lastname: Amon
mesh_terms: D018797:Cell Cycle Proteins / Q000378:metabolism / Q000502:physiology*; D002868:Chromosomal Proteins, Non-Histone / Q000378:metabolism; D008540:Meiosis / Q000502:physiology*; D010766:Phosphorylation; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism / Q000502:physiology*; D059748:Proteolysis; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism / Q000502:physiology*; D013379:Substrate Specificity
chemical_list: D018797:Cell Cycle Proteins; D002868:Chromosomal Proteins, Non-Histone; C502898:REC8 protein, S cerevisiae; C097192:SPO13 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D017346:Protein Serine-Threonine Kinases; C081927:CDC5 protein, S cerevisiae
references.citation: Science. 2003 Apr 18;300(5618):482-6
references.pmid: 12663816
references.citation: Nat Rev Mol Cell Biol. 2004 Dec;5(12):983-97
references.pmid: 15573136
references.citation: Cell. 2008 Apr 18;133(2):280-91
references.pmid: 18423199
references.citation: Cell. 2002 Jan 25;108(2):207-20
references.pmid: 11832211
references.citation: Dev Cell. 2003 Apr;4(4):535-48
references.pmid: 12689592
references.citation: Annu Rev Genet. 2004;38:203-32
references.pmid: 15568976
references.citation: Chromosoma. 2000;109(1-2):27-34
references.pmid: 10855492
references.citation: J Biol Chem. 2007 Jul 6;282(27):19710-5
references.pmid: 17493939
references.citation: Science. 2003 Feb 21;299(5610):1228-31
references.pmid: 12595692
references.citation: Dev Cell. 2010 Mar 16;18(3):397-409
references.pmid: 20230747
references.citation: Nat Rev Mol Cell Biol. 2009 Apr;10(4):265-75
references.pmid: 19305416
references.citation: Cell. 2008 Nov 14;135(4):662-78
references.pmid: 19013276
references.citation: Chem Biol. 2007 Nov;14(11):1261-72
references.pmid: 18022565
references.citation: Science. 2000 Jul 14;289(5477):300-3
references.pmid: 10894778
references.citation: Biochim Biophys Acta. 2004 Nov 29;1695(1-3):133-70
references.pmid: 15571813
references.citation: Cytogenet Genome Res. 2011;133(2-4):234-42
references.pmid: 21273764
references.citation: Curr Biol. 2002 Jun 4;12(11):944-50
references.pmid: 12062061
references.citation: Cell. 2001 Nov 30;107(5):655-65
references.pmid: 11733064
references.citation: Cell. 2012 Oct 26;151(3):603-18
references.pmid: 23101628
references.citation: Curr Biol. 2004 Dec 29;14(24):2183-96
references.pmid: 15620645
references.citation: Dev Cell. 2003 May;4(5):711-26
references.pmid: 12737806
references.citation: Curr Biol. 2004 Dec 29;14(24):2168-82
references.pmid: 15620644
references.citation: Nat Cell Biol. 2003 May;5(5):480-5
references.pmid: 12717442
references.citation: Cell. 2001 May 18;105(4):459-72
references.pmid: 11371343
references.citation: Cell. 2000 Oct 27;103(3):387-98
references.pmid: 11081626
references.citation: Elife. 2012 Dec 18;1:e00117
references.pmid: 23275833
references.citation: Genetics. 1990 Jul;125(3):475-85
references.pmid: 2199318
references.citation: Genetics. 1993 Oct;135(2):297-308
references.pmid: 8243995
references.citation: Dev Cell. 2003 May;4(5):727-39
references.pmid: 12737807
references.citation: Genes Dev. 2008 Oct 1;22(19):2627-32
references.pmid: 18832066
references.citation: Mol Biol Cell. 2009 Feb;20(3):1030-47
references.pmid: 19073884
references.citation: J Cell Biol. 2002 Apr 29;157(3):367-79
references.pmid: 11970961
references.citation: Nature. 2006 May 25;441(7092):532-6
references.pmid: 16672979