title: [The 2A protease of enterovirus 71 cleaves nup62 to inhibit nuclear transport].
abstract: To study the impact of the enterovirus 71(EV71) on the nuclear transport mechanism,The pGFP-NLS vector with nuclear location signal(NLS) was constructed, RD cells transfected by the pGFP-NLS vector were inoculated with the EV71 or cotransfected by EV71-2A vector. The results showed that GFP protein with NLS was expressed in the cytoplasm due to the inhibition of nuclear transport. In order to further study the mechanism of the EV71 to prevent nuclear transport,Nup62 was detected by Western blotting after RD cells were infected with EV71 or transfected by EV71-2A vector. The results showed that decreased expression of Nup62 could be detected after infection with EV71 and transfection by EV71-2A vector. This study demonstrates that the cleavage of Nup62 by EV71 2A protease may be the mechanism of nuclear transport inhibition.
authors.affiliation: Department of Pathology, Shanxi Medical University, Taiyuan 030001, China. 317546164@qq.com
authors.forename: Ya-Zhou
authors.initials: YZ
authors.lastname: Zhang
authors.forename: Xing
authors.initials: X
authors.lastname: Gan
authors.forename: Juan
authors.initials: J
authors.lastname: Song
authors.forename: Peng
authors.initials: P
authors.lastname: Sun
authors.forename: Qin-Qin
authors.initials: QQ
authors.lastname: Song
authors.forename: Gong-Qi
authors.initials: GQ
authors.lastname: Li
authors.forename: Lin-Jun
authors.initials: LJ
authors.lastname: Sheng
authors.forename: Bao-Dong
authors.initials: BD
authors.lastname: Wang
authors.forename: Ming-Zhi
authors.initials: MZ
authors.lastname: Lu
authors.forename: Ling-Min
authors.initials: LM
authors.lastname: Li
authors.forename: Jun
authors.initials: J
authors.lastname: Han
mesh_terms: D021581:Active Transport, Cell Nucleus; D045744:Cell Line, Tumor; D002467:Cell Nucleus / Q000378:metabolism*; D029821:Enterovirus A, Human / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D004769:Enterovirus Infections / Q000821:virology*; D015967:Gene Expression Regulation, Viral; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006801:Humans; D008562:Membrane Glycoproteins / Q000378:metabolism*; D019913:Nuclear Localization Signals / Q000378:metabolism*; D028861:Nuclear Pore Complex Proteins / Q000378:metabolism*; D010447:Peptide Hydrolases / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D014162:Transfection
chemical_list: D008562:Membrane Glycoproteins; D019913:Nuclear Localization Signals; D028861:Nuclear Pore Complex Proteins; D011993:Recombinant Fusion Proteins; C049312:nuclear pore protein p62; D049452:Green Fluorescent Proteins; D010447:Peptide Hydrolases