title: Effects of bioactive-rich extracts of pomegranate, persimmon, nettle, dill, kale and Sideritis and isolated bioactives on arachidonic acid induced markers of platelet activation and aggregation.
abstract: BACKGROUND
  The beneficial effect of fruit- and vegetable-rich diets on cardiovascular health is partly attributed to the effect of their bioactive compounds on platelet function. The aim of this study was to investigate the effects of bioactive-rich plant extracts and isolated bioactive metabolites on platelet function. Blood samples from healthy subjects (n = 4) and subjects with metabolic syndrome (n = 4) were treated with six extracts of bioactive-rich plants consumed as traditional foods in the Black Sea region, or with human metabolites of the bioactives quercetin and sulforaphane. Markers of arachidonic acid induced platelet activation and platelet-leucocyte aggregation were assessed using flow cytometry.
  
  
  RESULTS
  In subjects with metabolic syndrome, kale extract significantly inhibited agonist induced P-selectin expression (P = 0.004). Sulforaphane-cysteine-glycine, a human plasma metabolite of the related glucosinolate, glucoraphanin, significantly inhibited P-selectin and GPIIb-IIIa expression (P = 0.020 and 0.024, respectively) and platelet-neutrophil aggregation (P = 0.027). Additionally, pomegranate extract significantly inhibited GPIIb-IIIa expression (P = 0.046) in subjects with metabolic syndrome. In healthy subjects only dill extract significantly inhibited agonist induced P-selectin expression (P = 0.025).
  
  
  CONCLUSIONS
  These data show that bioactive-rich extracts of kale and pomegranate that are consumed as traditional plant foods of Black Sea area countries were effective in modulating platelet function.
authors.affiliation: Institute for Medical Research, University of Belgrade, Belgrade, Serbia.
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Konić-Ristić
authors.forename: Tatjana
authors.initials: T
authors.lastname: Srdić-Rajić
authors.forename: Nevena
authors.initials: N
authors.lastname: Kardum
authors.forename: Vesna
authors.initials: V
authors.lastname: Aleksić-Veličković
authors.forename: Paul A
authors.initials: PA
authors.lastname: Kroon
authors.forename: Wendy J
authors.initials: WJ
authors.lastname: Hollands
authors.forename: Paul W
authors.initials: PW
authors.lastname: Needs
authors.forename: Nadiya
authors.initials: N
authors.lastname: Boyko
authors.forename: Osman
authors.initials: O
authors.lastname: Hayran
authors.forename: Mariam
authors.initials: M
authors.lastname: Jorjadze
authors.forename: Maria
authors.initials: M
authors.lastname: Glibetić
mesh_terms: D028524:Anethum graveolens / Q000737:chemistry; D016718:Arachidonic Acid / Q000494:pharmacology; D059038:Black Sea; D001792:Blood Platelets / Q000187:drug effects; D001937:Brassica / Q000737:chemistry*; D003469:Culture; D029788:Diospyros / Q000737:chemistry; D005502:Food; D005638:Fruit / Q000737:chemistry; D006801:Humans; D017879:Isothiocyanates / Q000097:blood / Q000494:pharmacology; D029561:Lythraceae / Q000737:chemistry*; D024821:Metabolic Syndrome / Q000097:blood; D019007:P-Selectin / Q000097:blood; D010936:Plant Extracts / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D015539:Platelet Activation / Q000187:drug effects*; D010974:Platelet Aggregation / Q000187:drug effects*; D019039:Platelet Glycoprotein GPIIb-IIIa Complex / Q000032:analysis; D011794:Quercetin / Q000097:blood / Q000494:pharmacology; D031365:Sideritis / Q000737:chemistry; D013454:Sulfoxides; D026982:Urtica dioica / Q000737:chemistry
chemical_list: D017879:Isothiocyanates; D019007:P-Selectin; D010936:Plant Extracts; D019039:Platelet Glycoprotein GPIIb-IIIa Complex; D013454:Sulfoxides; D016718:Arachidonic Acid; D011794:Quercetin; C016766:sulforaphane
keywords: flow cytometry; in vitro; platelet activation; platelet aggregation; polyphenols