title: Identification and characterization of CHCHD1, AURKAIP1, and CRIF1 as new members of the mammalian mitochondrial ribosome.
abstract: Defects in mitochondrial ribosomal proteins (MRPs) cause various diseases in humans. Because of the essential role of MRPs in synthesizing the essential subunits of oxidative phosphorylation (OXPHOS) complexes, identifying all of the protein components involved in the mitochondrial translational machinery is critical. Initially, we identified 79 MRPs; however, identifying MRPs with no clear homologs in bacteria and yeast mitochondria was challenging, due to limited availability of expressed sequence tags (ESTs) in the databases available at that time. With the improvement in genome sequencing and increased sensitivity of mass spectrometry (MS)-based technologies, we have established four previously known proteins as MRPs and have confirmed the identification of ICT1 (MRP58) as a ribosomal protein. The newly identified MRPs are MRPS37 (Coiled-coil-helix-coiled-coil-helix domain containing protein 1-CHCHD1), MRPS38 (Aurora kinase A interacting protein1, AURKAIP1), MRPS39 (Pentatricopeptide repeat-containing protein 3, PTCD3), in the small subunit and MRPL59 (CR-6 interacting factor 1, CRIF1) in the large subunit. Furthermore, we have demonstrated the essential roles of CHCHD1, AURKAIP1, and CRIF1in mitochondrial protein synthesis by siRNA knock-down studies, which had significant effects on the expression of mitochondrially encoded proteins.
authors.affiliation: Department of Biochemistry and Microbiology, Joan C. Edwards School of Medicine, Marshall University Huntington, WV, USA.
authors.forename: Emine C
authors.initials: EC
authors.lastname: Koc
authors.forename: Huseyin
authors.initials: H
authors.lastname: Cimen
authors.forename: Beril
authors.initials: B
authors.lastname: Kumcuoglu
authors.forename: Nadiah
authors.initials: N
authors.lastname: Abu
authors.forename: Gurler
authors.initials: G
authors.lastname: Akpinar
authors.forename: Md Emdadul
authors.initials: ME
authors.lastname: Haque
authors.forename: Linda L
authors.initials: LL
authors.lastname: Spremulli
authors.forename: Hasan
authors.initials: H
authors.lastname: Koc
keywords: 55S; MRPL58 (ICT1); MRPL59 (CRIF1); MRPS37 (CHCHD1); MRPS38 (AURKAIP1); MRPS39 (PTCD3); mitochondrial ribosomal proteins; mitochondrial translation
references.citation: Biochim Biophys Acta. 2012 Sep-Oct;1819(9-10):1055-66
references.pmid: 22480953
references.citation: Nucleic Acids Res. 2012 May;40(9):4097-109
references.pmid: 22238376
references.citation: Mol Cell Biol. 2005 Aug;25(15):6603-16
references.pmid: 16024796
references.citation: Gene. 2005 Jul 18;354:147-51
references.pmid: 15908146
references.citation: Biochim Biophys Acta. 2011 Sep;1807(9):1198-205
references.pmid: 21708121
references.citation: Eur J Biochem. 1996 Nov 1;241(3):779-86
references.pmid: 8944766
references.citation: Eur J Hum Genet. 2011 Apr;19(4):394-9
references.pmid: 21189481
references.citation: Eur J Biochem. 1976 Sep;68(1):301-11
references.pmid: 964267
references.citation: EMBO J. 2010 Mar 17;29(6):1116-25
references.pmid: 20186120
references.citation: J Cell Biochem. 2005 Feb 15;94(3):611-26
references.pmid: 15547950
references.citation: J Biol Chem. 2001 Jun 15;276(24):21724-36
references.pmid: 11279069
references.citation: Histol Histopathol. 1998 Oct;13(4):1233-42
references.pmid: 9810514
references.citation: FEBS Lett. 2009 Jun 18;583(12):1853-8
references.pmid: 19427859
references.citation: J Biol Chem. 2003 Jul 25;278(30):28079-88
references.pmid: 12716909
references.citation: J Biol Chem. 2000 Oct 20;275(42):32585-91
references.pmid: 10938081
references.citation: Biochem Biophys Res Commun. 1973 Sep 5;54(1):308-14
references.pmid: 4741570
references.citation: Protein Sci. 2008 Feb;17(2):251-60
references.pmid: 18227431
references.citation: Biochimie. 1991 Jun;73(6):827-35
references.pmid: 1764527
references.citation: Cytogenet Cell Genet. 1999;87(1-2):85-8
references.pmid: 10640817
references.citation: J Mol Biol. 2010 Nov 26;404(2):260-73
references.pmid: 20869366
references.citation: Mol Biol Rep. 1994 May;19(3):183-94
references.pmid: 7969106
references.citation: J Biol Chem. 1998 Dec 25;273(52):34828-36
references.pmid: 9857009
references.citation: J Biol Chem. 2001 Nov 23;276(47):43958-69
references.pmid: 11551941
references.citation: Nat Biotechnol. 2010 Dec;28(12):1248-50
references.pmid: 21139605
references.citation: J Biol Chem. 2006 Feb 10;281(6):3743-51
references.pmid: 16339141
references.citation: Biochem J. 2010 Sep 15;430(3):551-8
references.pmid: 20604745
references.citation: Biochemistry. 1999 Dec 14;38(50):16569-77
references.pmid: 10600119
references.citation: J Biol Chem. 1999 Dec 17;274(51):36043-51
references.pmid: 10593885
references.citation: Cell. 2003 Oct 3;115(1):97-108
references.pmid: 14532006
references.citation: Biochem Biophys Res Commun. 1999 Dec 9;266(1):141-6
references.pmid: 10581179
references.citation: Curr Genet. 1997 Mar;31(3):228-34
references.pmid: 9065385
references.citation: J Biol Chem. 2010 Mar 5;285(10):7417-29
references.pmid: 20042612
references.citation: Mitochondrion. 2003 Mar;2(4):277-91
references.pmid: 16120328
references.citation: Trends Genet. 2005 Jun;21(6):312-4
references.pmid: 15922826
references.citation: Biochim Biophys Acta. 2012 Sep-Oct;1819(9-10):1035-54
references.pmid: 22172991
references.citation: J Biol Chem. 2002 Nov 22;277(47):45558-65
references.pmid: 12244051
references.citation: J Biol Chem. 1982 Aug 10;257(15):8788-94
references.pmid: 7047527
references.citation: Nucleic Acids Res. 2012 Jul;40(13):6109-21
references.pmid: 22453275
references.citation: Methods Mol Biol. 2009;554:143-62
references.pmid: 19513673
references.citation: BMC Genomics. 2006 Oct 20;7:268
references.pmid: 17054779
references.citation: J Biol Chem. 2010 Jul 9;285(28):21258-68
references.pmid: 20427290
references.citation: Methods Enzymol. 1996;264:197-211
references.pmid: 8965693
references.citation: J Med Genet. 2007 Dec;44(12):784-6
references.pmid: 17873122
references.citation: Nucleic Acids Res. 2007;35(14):4686-703
references.pmid: 17604309
references.citation: Ann Neurol. 2004 Nov;56(5):734-8
references.pmid: 15505824
references.citation: Biochim Biophys Acta. 2011 Dec;1814(12):1779-84
references.pmid: 22015679
references.citation: J Biol Chem. 2001 Jun 1;276(22):19363-74
references.pmid: 11279123
references.citation: J Proteome Res. 2009 Oct;8(10):4789-98
references.pmid: 19702336
references.citation: Cell Metab. 2012 Aug 8;16(2):274-83
references.pmid: 22819524
references.citation: J Proteome Res. 2009 Jul;8(7):3390-402
references.pmid: 19469554
references.citation: Mol Cell Biol. 2001 Apr;21(7):2359-72
references.pmid: 11259585
references.citation: Eur J Biochem. 1992 Jun 1;206(2):373-80
references.pmid: 1597181
references.citation: Hum Mutat. 2011 Nov;32(11):1225-31
references.pmid: 21786366
references.citation: Methods Mol Biol. 2007;372:265-75
references.pmid: 18314732
references.citation: Mol Cell Proteomics. 2005 Sep;4(9):1265-72
references.pmid: 15958392
references.citation: Eur J Biochem. 1995 Dec 15;234(3):843-8
references.pmid: 8575443
references.citation: Genomics. 2001 Sep;77(1-2):65-70
references.pmid: 11543634
references.citation: Bioorg Khim. 2004 Sep-Oct;30(5):499-506
references.pmid: 15562971
references.citation: Clin Cancer Res. 2001 Aug;7(8):2480-9
references.pmid: 11489830