title: Selective light-triggered release of DNA from gold nanorods switches blood clotting on and off.
abstract: Blood clotting is a precise cascade engineered to form a clot with temporal and spatial control. Current control of blood clotting is achieved predominantly by anticoagulants and thus inherently one-sided. Here we use a pair of nanorods (NRs) to provide a two-way switch for the blood clotting cascade by utilizing their ability to selectively release species on their surface under two different laser excitations. We selectively trigger release of a thrombin binding aptamer from one nanorod, inhibiting blood clotting and resulting in increased clotting time. We then release the complementary DNA as an antidote from the other NR, reversing the effect of the aptamer and restoring blood clotting. Thus, the nanorod pair acts as an on/off switch. One challenge for nanobiotechnology is the bio-nano interface, where coronas of weakly adsorbed proteins can obscure biomolecular function. We exploit these adsorbed proteins to increase aptamer and antidote loading on the nanorods.
authors.affiliation: Department of Mechanical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, United States of America.
authors.forename: Helena
authors.initials: H
authors.lastname: de Puig
authors.forename: Anna
authors.initials: A
authors.lastname: Cifuentes Rius
authors.forename: Dorma
authors.initials: D
authors.lastname: Flemister
authors.forename: Salmaan H
authors.initials: SH
authors.lastname: Baxamusa
authors.forename: Kimberly
authors.initials: K
authors.lastname: Hamad-Schifferli
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry / Q000494:pharmacology; D001777:Blood Coagulation / Q000187:drug effects / Q000528:radiation effects*; D004247:DNA / Q000737:chemistry* / Q000494:pharmacology*; D006046:Gold / Q000737:chemistry*; D006801:Humans; D008027:Light; D053768:Metal Nanoparticles / Q000737:chemistry*; D036103:Nanotechnology; D043942:Nanotubes / Q000737:chemistry*
chemical_list: D052157:Aptamers, Nucleotide; C081676:thrombin aptamer; D006046:Gold; D004247:DNA
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