title: Massive indexed parallel identification of transposon flanking sequences.
abstract: The large scale sequencing of insertion element flanking sequences has revolutionized reverse genetics in plant research: Insertion mutants can now simply be identified in silico by BLAST searching the resulting flanking sequence databases. The development of next-generation sequencing technologies has further facilitated the creation of flanking sequence collections derived from entire mutant populations. Here we describe a highly efficient and widely applicable method that we developed to amplify, sequence, and identify dTph1 transposon flanking sequences from a library of 1000 Petunia W138 individuals simultaneously.
authors.affiliation: UMR 5667 CNRS-INRA-ENS Lyon-Unversité Lyon I, RDP Laboratory, ENS Lyon, Lyon, Cedex, France.
authors.forename: Michiel
authors.initials: M
authors.lastname: Vandenbussche
authors.forename: Jan
authors.initials: J
authors.lastname: Zethof
authors.forename: Tom
authors.initials: T
authors.lastname: Gerats
mesh_terms: D004251:DNA Transposable Elements / Q000235:genetics*; D018744:DNA, Plant / Q000235:genetics / Q000302:isolation & purification; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D021141:Nucleic Acid Amplification Techniques; D032306:Petunia / Q000235:genetics; D017422:Sequence Analysis, DNA / Q000379:methods*
chemical_list: D004251:DNA Transposable Elements; D018744:DNA, Plant