title: Delivery systems for gene therapy.
abstract: The structure of DNA was unraveled by Watson and Crick in 1953, and two decades later Arber, Nathans and Smith discovered DNA restriction enzymes, which led to the rapid growth in the field of recombinant DNA technology. From expressing cloned genes in bacteria to expressing foreign DNA in transgenic animals, DNA is now slated to be used as a therapeutic agent to replace defective genes in patients suffering from genetic disorders or to kill tumor cells in cancer patients. Gene therapy provides modern medicine with new perspectives that were unthinkable two decades ago. Progress in molecular biology and especially, molecular medicine is now changing the basics of clinical medicine. A variety of viral and non-viral possibilities are available for basic and clinical research. This review summarizes the delivery routes and methods for gene transfer used in gene therapy.
authors.affiliation: Department of Oral and Maxillofacial Surgery, CSMSS Dental College, Aurangabad, Maharashtra, India.
authors.forename: Shrikant
authors.initials: S
authors.lastname: Mali
keywords: Gene therapy; liposomes; viral vectors
references.citation: J Dent Res. 2003 Jan;82(1):11-6
references.pmid: 12508038
references.citation: Proc Natl Acad Sci U S A. 1997 Nov 25;94(24):12744-6
references.pmid: 9398067
references.citation: Med Res Rev. 2004 Nov;24(6):748-61
references.pmid: 15250039
references.citation: Head Neck Oncol. 2009 Mar 30;1:7
references.pmid: 19331651
references.citation: Bratisl Lek Listy. 2011;112(8):428-34
references.pmid: 21863612
references.citation: Cancer Control. 2002 Sep-Oct;9(5):369-78
references.pmid: 12410176
references.citation: Nature. 1998 Apr 30;392(6679 Suppl):25-30
references.pmid: 9579858
references.citation: J Biomed Biotechnol. 2003;2003(1):35-47
references.pmid: 12686721
references.citation: Med Sci Monit. 2005 Apr;11(4):RA110-21
references.pmid: 15795707
references.citation: Diagn Interv Radiol. 2005 Jun;11(2):113-8
references.pmid: 15957100
references.citation: Med Oral Patol Oral Cir Bucal. 2008 Jan 01;13(1):E15-21
references.pmid: 18167474