title: A monoclonal antibody against human MUDENG protein.
abstract: MUDENG (mu-2-related death-inducing gene, MuD) encodes a predicted ∼54-kDa protein in humans, considered to be involved in trafficking proteins from endosomes toward other membranous compartments as well as in inducing cell death. Here we report on the generation of a mouse monoclonal antibody (MAb) against the middle domain of human (h) MuD. This IgG sub 1 MAb, named M3H9, recognizes residues 244-326 in the middle domain of the MuD protein. Thus, the MuD proteins expressed in an astroglioma cell line and primary astrocytes can be detected by the M3H9 MAb. We showed that M3H9 MAb can be useful in enzyme-linked immunosorbent assay (ELISA) and immunoblot experiments. In addition, M3H9 MAb can detect the expression of the MuD protein in formalin-fixed, paraffin-embedded mouse ovary and uterus tissues. These results indicate that the MuD MAb M3H9 could be useful as a new biomarker of hereditary spastic paraplegia and other related diseases.
authors.affiliation: Division of Animal Bioscience and Technology, College of Animal Bioscience and Biotechnology/Animal Resources Research Center, Konkuk University, Seoul, Korea.
authors.forename: Yadav
authors.initials: Y
authors.lastname: Wagley
authors.forename: Jun-Ha
authors.initials: JH
authors.lastname: Choi
authors.forename: Dimuthu Dhammika
authors.initials: DD
authors.lastname: Wickramanayake
authors.forename: Geun-Yeol
authors.initials: GY
authors.lastname: Choi
authors.forename: Chang-Kyu
authors.initials: CK
authors.lastname: Kim
authors.forename: Tae-Hyoung
authors.initials: TH
authors.lastname: Kim
authors.forename: Jae-Wook
authors.initials: JW
authors.lastname: Oh
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology* / Q000302:isolation & purification; D000917:Antibody Formation; D000918:Antibody Specificity; D051017:Apoptosis Regulatory Proteins / Q000276:immunology*; D001253:Astrocytes / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D015153:Blotting, Western; D002478:Cells, Cultured; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D006825:Hybridomas / Q000276:immunology*; D007124:Immunoenzyme Techniques; D051379:Mice; D008807:Mice, Inbred BALB C; D010053:Ovary / Q000276:immunology / Q000378:metabolism; D051381:Rats; D011994:Recombinant Proteins; D014599:Uterus / Q000276:immunology / Q000378:metabolism
chemical_list: C529058:AP5M1 protein, human; D000911:Antibodies, Monoclonal; D051017:Apoptosis Regulatory Proteins; D011994:Recombinant Proteins
references.citation: PLoS Biol. 2010 Jun 29;8(6):e1000408
references.pmid: 20613862
references.citation: Trends Pharmacol Sci. 2009 Mar;30(3):138-47
references.pmid: 19187977
references.citation: Proteomics. 2004 Jun;4(6):1633-49
references.pmid: 15174133
references.citation: PLoS Biol. 2011 Oct;9(10):e1001173
references.pmid: 22022232
references.citation: J Immunol. 2008 Sep 1;181(5):3167-76
references.pmid: 18713987
references.citation: Biochem Biophys Res Commun. 2008 Jun 6;370(3):504-8
references.pmid: 18395520
references.citation: Nucleic Acids Res. 2002 Aug 15;30(16):3609-14
references.pmid: 12177303
references.citation: PLoS Biol. 2011 Oct;9(10):e1001170
references.pmid: 22022230