title: One-pot synthesis of iron oxide nanoparticles with functional silane shells: a versatile general precursor for conjugations and biomedical applications.
abstract: Iron oxide nanoparticles (IONPs) and their surface modifications with therapeutic or diagnostic (theranostic, TN) agents are of great interest. Here we present a novel one-pot synthesis of a versatile general TN precursor (aminosilane-coated IONPs [IONP-Sil(NH2)]) with surface amine groups. Surface functional group conversion to carboxylic acid was accomplished by conjugating poly(ethylene glycol) diacid to IONP-Sil(NH2). The NPs were characterized using powder X-ray diffraction (XRD), transmission electron microscopy (TEM), high-resolution TEM, selected area electron diffraction (SAED), X-ray photoelectron spectroscopy (XPS), and Fourier transform infrared (FT-IR) spectroscopy. Biocompatibility and cell uptake profile of the nanoparticles were evaluated in-vitro using cultured liver cells (HepG2). Oleylamine (hydrophobic) and bovine serum albumin (BSA) as model drugs were attached to IONP-Sil-PEG(COOH). The ability of IONP-Sil(NH2) to bind small interfering RNA (siRNA) is also shown.
authors.affiliation: Department of Chemistry, University of Manitoba, Winnipeg, MB, R3T 2N2, Canada.
authors.forename: Vinith
authors.initials: V
authors.lastname: Yathindranath
authors.forename: Zhizhi
authors.initials: Z
authors.lastname: Sun
authors.forename: Matthew
authors.initials: M
authors.lastname: Worden
authors.forename: Lynda J
authors.initials: LJ
authors.lastname: Donald
authors.forename: James A
authors.initials: JA
authors.lastname: Thliveris
authors.forename: Donald W
authors.initials: DW
authors.lastname: Miller
authors.forename: Torsten
authors.initials: T
authors.lastname: Hegmann
mesh_terms: D005290:Ferric Compounds / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry*; D012821:Silanes / Q000737:chemistry*
chemical_list: D005290:Ferric Compounds; D012821:Silanes; C000499:ferric oxide