title: Antiviral activity of Folium isatidis derived extracts in vitro and in vivo.
abstract: Folium isatidis is a native Chinese herbaceous plant widely used for medicinal purposes for thousands of years. However, few studies have focused on the leaves of Isatis indigotica. In this report, we isolated a series of four fractions (I-IV) from Folium isatidis and explored the antiviral activity of each tested extract. The extracts were active against a panel of RNA and DNA viruses in vitro, namely influenza A virus (IAV), coxsackie virus B3 (CVB3), respiratory syncytial virus (RSV), and adenovirus type 7 (Ad-7). Oral administration of 200 mg/kg/d of fraction III in mice exerted strong antiviral effects in viral replication, accompanied by prolonged survival rate, attenuated lung tissue damage as well as significant reductions in pulmonary virus titers and lung index. Our results provide the first biochemical evidence that Folium isatidis and its extracts could be used as potential antiviral agent in the postexposure prophylaxis for multiple viral infections.
authors.affiliation: State Key Laboratory of Virology, Institute of Medical Virology, Research Center of Food and Drug Evaluation, National Laboratory of Antiviral and Tumor of Traditional Chinese Medicine, School of Medicine, Wuhan University, Wuhan, China.
authors.forename: You-Ping
authors.initials: YP
authors.lastname: Deng
authors.forename: Yuan-Yuan
authors.initials: YY
authors.lastname: Liu
authors.forename: Zhao
authors.initials: Z
authors.lastname: Liu
authors.forename: Jin
authors.initials: J
authors.lastname: Li
authors.forename: Ling-Min
authors.initials: LM
authors.lastname: Zhao
authors.forename: Hong
authors.initials: H
authors.lastname: Xiao
authors.forename: Xiao-Hua
authors.initials: XH
authors.lastname: Ding
authors.forename: Zhan-Qiu
authors.initials: ZQ
authors.lastname: Yang
mesh_terms: D000256:Adenoviridae / Q000187:drug effects*; D000818:Animals; D000998:Antiviral Agents / Q000302:isolation & purification / Q000494:pharmacology*; D045744:Cell Line, Tumor; D004285:Dogs; D029822:Enterovirus B, Human / Q000187:drug effects*; D006801:Humans; D009980:Influenza A virus / Q000187:drug effects*; D031219:Isatis; D008168:Lung / Q000187:drug effects / Q000821:virology; D061985:Madin Darby Canine Kidney Cells; D051379:Mice; D009976:Orthomyxoviridae Infections; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D018515:Plant Leaves; D011014:Pneumonia; D012136:Respiratory Syncytial Viruses / Q000187:drug effects*; D014779:Virus Replication / Q000187:drug effects
chemical_list: D000998:Antiviral Agents; D010936:Plant Extracts