title: Laboratory investigation of an Escherichia coli O157:H7 strain possessing a vtx2c gene with an IS1203 variant insertion sequence isolated from an asymptomatic food handler in Japan.
abstract: We isolated an Escherichia coli O157:H7 strain that was negative for verocytotoxin production, but positive for the vtx2 gene using commercial kits, from an asymptomatic food handler. The laboratory investigations revealed that a 1310-bp insertion sequence, IS1203 variant, was present in the B subunit-coding region of the vtx2c gene.
authors.affiliation: Division of Bacteriology, Osaka Prefectural Institute of Public Health, Osaka 537-0025, Japan. Electronic address: harada@iph.pref.osaka.jp.
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Harada
authors.forename: Yuji
authors.initials: Y
authors.lastname: Hirai
authors.forename: Takeshi
authors.initials: T
authors.lastname: Itou
authors.forename: Mizuho
authors.initials: M
authors.lastname: Hayashida
authors.forename: Kazuko
authors.initials: K
authors.lastname: Seto
authors.forename: Masumi
authors.initials: M
authors.lastname: Taguchi
authors.forename: Yuko
authors.initials: Y
authors.lastname: Kumeda
mesh_terms: D000328:Adult; D002353:Carrier State / Q000382:microbiology*; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D004927:Escherichia coli Infections / Q000382:microbiology*; D019453:Escherichia coli O157 / Q000235:genetics* / Q000472:pathogenicity; D005260:Female; D005511:Food Handling; D005798:Genes, Bacterial; D006801:Humans; D016254:Mutagenesis, Insertional; D022603:Shiga Toxins / Q000235:genetics*; D055815:Young Adult
chemical_list: D004269:DNA, Bacterial; D022603:Shiga Toxins
keywords: E. coli O157:H7; IS1203v