title: DNA electrophoresis: historical and theoretical perspectives.
abstract: The technique of gel electrophoresis is now firmly established as a routine laboratory method for analyzing DNA. Here, we describe the development of the methodology as well as a brief explanation of how the technique works. There is a short introduction to pulsed-field agarose gel electrophoresis, which represents a critical advancement in the method that facilitates the analysis of very large fragments of DNA. Finally, theoretical considerations are included.
authors.affiliation: School of Chemistry, University of Edinburgh, Edinburgh, UK.
authors.forename: Gareth A
authors.initials: GA
authors.lastname: Roberts
authors.forename: David T F
authors.initials: DT
authors.lastname: Dryden
mesh_terms: D004247:DNA / Q000737:chemistry*; D004587:Electrophoresis, Agar Gel / Q000379:methods*; D016521:Electrophoresis, Gel, Pulsed-Field / Q000379:methods*; D006801:Humans; D012685:Sepharose / Q000737:chemistry
chemical_list: D004247:DNA; D012685:Sepharose