title: Arxula adeninivorans recombinant adenine deaminase and its application in the production of food with low purine content.
abstract: OBJECTIVE
  Construction of a transgenic Arxula adeninivorans strain that produces a high concentration of adenine deaminase and investigation into the application of the enzyme in the production of food with low purine content.
  
  
  RESULTS
  The A. adeninivorans AADA gene, encoding adenine deaminase, was expressed in this yeast under the control of the strong inducible nitrite reductase promoter using the Xplor(®) 2 transformation/expression platform. The recombinant enzyme was biochemically characterized and was found to have a pH range of 5.5-7.5 and temperature range of 34-46 °C with medium thermostability. A beef broth was treated with the purified enzyme resulting in the concentration of adenine decreasing from 70.4 to 0.4 mg l(-1).
  
  
  CONCLUSIONS
  It was shown that the production of adenine deaminase by A. adeninivorans can be increased and that the recombinant adenine deaminase can be used to lower the adenine content in the food.
  
  
  CONCLUSIONS
  Adenine deaminase is one component of an enzymatic system that can reduce the production of uric acid from food constituents. This study gives details on the expression, characterization and application of the enzyme and thus provides evidence that supports the further development of the system.
authors.affiliation: Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Gatersleben, Germany.
authors.forename: D A
authors.initials: DA
authors.lastname: Jankowska
authors.forename: K
authors.initials: K
authors.lastname: Faulwasser
authors.forename: A
authors.initials: A
authors.lastname: Trautwein-Schult
authors.forename: A
authors.initials: A
authors.lastname: Cordes
authors.forename: P
authors.initials: P
authors.lastname: Hoferichter
authors.forename: C
authors.initials: C
authors.lastname: Klein
authors.forename: R
authors.initials: R
authors.lastname: Bode
authors.forename: K
authors.initials: K
authors.lastname: Baronian
authors.forename: G
authors.initials: G
authors.lastname: Kunze
mesh_terms: D000225:Adenine / Q000032:analysis; D000619:Aminohydrolases / Q000737:chemistry* / Q000235:genetics; D000818:Animals; D002417:Cattle; D005516:Food Microbiology; D005800:Genes, Fungal; D030781:Organisms, Genetically Modified; D011401:Promoter Regions, Genetic; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics; D004718:Saccharomycetales / Q000201:enzymology* / Q000235:genetics
chemical_list: D011994:Recombinant Proteins; D000619:Aminohydrolases; C018774:adenine deaminase; D000225:Adenine
keywords: Arxula adeninivorans; adenine deaminase; food treatment; gout; hyperuricemia; yeast