title: Biochemical markers assisted screening of Fusarium wilt resistant Musa paradisiaca (L.) cv. puttabale micropropagated clones.
abstract: An efficient protocol was standardized for screening of panama wilt resistant Musa paradisiaca cv. Puttabale clones, an endemic cultivar of Karnataka, India. The synergistic effect of 6-benzyleaminopurine (2 to 6 mg/L) and thidiazuron (0.1 to 0.5 mg/L) on MS medium provoked multiple shoot induction from the excised meristem. An average of 30.10 +/- 5.95 shoots was produced per propagule at 4 mg/L 6-benzyleaminopurine and 0.3 mg/L thidiazuron concentrations. Elongation of shoots observed on 5 mg/L BAP augmented medium with a mean length of 8.38 +/- 0.30 shoots per propagule. For screening of disease resistant clones, multiple shoot buds were mutated with 0.4% ethyl-methane-sulfonate and cultured on MS medium supplemented with Fusarium oxysporum f. sp. cubense (FOC) culture filtrate (5-15%). Two month old co-cultivated secondary hardened plants were used for screening of disease resistance against FOC by the determination of biochemical markers such as total phenol, phenylalanine ammonia lyase, oxidative enzymes like peroxidase, polyphenol oxidase, catalase and PR-proteins like chitinase, beta-1-3 glucanase activities. The mutated clones of M. paradisiaca cv. Puttabale cultured on FOC culture filtrate showed significant increase in the levels of biochemical markers as an indicative of acquiring disease resistant characteristics to FOC wilt.
authors.affiliation: P.G. Department of Studies and Research in Biotechnology and Bioinformatics, Kuvempu University, Shankaraghatta 577 451, India.
authors.lastname: Venkatesh
authors.forename: V
authors.initials: V
authors.lastname: Krishna
authors.forename: K Girish
authors.initials: KG
authors.lastname: Kumar
authors.forename: K
authors.initials: K
authors.lastname: Pradeepa
authors.forename: S R Santosh
authors.initials: SR
authors.lastname: Kumar
authors.forename: R Shashi
authors.initials: RS
authors.lastname: Kumar
mesh_terms: D001593:Benzyl Compounds; D015415:Biomarkers / Q000032:analysis*; D002478:Cells, Cultured; D005670:Fusarium / Q000235:genetics / Q000472:pathogenicity*; D054884:Host-Pathogen Interactions; D007701:Kinetin / Q000494:pharmacology; D028521:Musa / Q000187:drug effects / Q000235:genetics / Q000382:microbiology*; D010671:Phenylurea Compounds / Q000494:pharmacology; D010935:Plant Diseases / Q000235:genetics / Q000276:immunology* / Q000382:microbiology*; D018520:Plant Shoots / Q000187:drug effects / Q000235:genetics / Q000382:microbiology*; D011687:Purines; D013830:Thiadiazoles / Q000494:pharmacology
chemical_list: D001593:Benzyl Compounds; D015415:Biomarkers; D010671:Phenylurea Compounds; D011687:Purines; D013830:Thiadiazoles; C016785:thidiazuron; C480551:benzylaminopurine; D007701:Kinetin