title: miR-29 acts as a decoy in sarcomas to protect the tumor suppressor A20 mRNA from degradation by HuR.
abstract: In sarcoma, the activity of NF-κB (nuclear factor κB) reduces the abundance of the microRNA (miRNA) miR-29. The tumor suppressor A20 [also known as TNFAIP3 (tumor necrosis factor-α-induced protein 3)] inhibits an upstream activator of NF-κB and is often mutated in lymphomas. In a panel of human sarcoma cell lines, we found that the activation of NF-κB was increased and, although the abundance of A20 protein and mRNA was decreased, the gene encoding A20 was rarely mutated. The 3' untranslated region (UTR) of A20 mRNA has conserved binding sites for both of the miRNAs miR-29 and miR-125. Whereas the expression of miR-125 was increased in human sarcoma tissue, that of miR-29 was decreased in most samples. Overexpression of miR-125 decreased the abundance of A20 mRNA, whereas reconstituting miR-29 in sarcoma cell lines increased the abundance of A20 mRNA and protein. By interacting directly with the RNA binding protein HuR (human antigen R; also known as ELAVL1), miR-29 prevented HuR from binding to the A20 3'UTR and recruiting the RNA degradation complex RISC (RNA-induced silencing complex), suggesting that miR-29 can act as a decoy for HuR, thus protecting A20 transcripts. Decreased miR-29 and A20 abundance in sarcomas correlated with increased activity of NF-κB and decreased expression of genes associated with differentiation. Together, the findings reveal a unique role of miR-29 and suggest that its absence may contribute to sarcoma tumorigenesis.
authors.affiliation: Department of Molecular Virology, Immunology, and Medical Genetics, Human Cancer Genetics Program, The Ohio State University, Columbus, OH 43210, USA.
authors.forename: M Y
authors.initials: MY
authors.lastname: Balkhi
authors.affiliation: Department of Molecular Virology, Immunology, and Medical Genetics, Human Cancer Genetics Program, The Ohio State University, Columbus, OH 43210, USA.
authors.forename: Mumtaz Y
authors.initials: MY
authors.lastname: Balkhi
authors.forename: O Hans
authors.initials: OH
authors.lastname: Iwenofu
authors.forename: Nadine
authors.initials: N
authors.lastname: Bakkar
authors.forename: Katherine J
authors.initials: KJ
authors.lastname: Ladner
authors.forename: Dawn S
authors.initials: DS
authors.lastname: Chandler
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Houghton
authors.forename: Cheryl A
authors.initials: CA
authors.lastname: London
authors.forename: William
authors.initials: W
authors.lastname: Kraybill
authors.forename: Danilo
authors.initials: D
authors.lastname: Perrotti
authors.forename: Carlo M
authors.initials: CM
authors.lastname: Croce
authors.forename: Charles
authors.initials: C
authors.lastname: Keller
authors.forename: Denis C
authors.initials: DC
authors.lastname: Guttridge
mesh_terms: D020413:3' Untranslated Regions; D000818:Animals; D001665:Binding Sites; D019295:Computational Biology; D004268:DNA-Binding Proteins / Q000378:metabolism*; D051959:ELAV Proteins / Q000378:metabolism*; D015972:Gene Expression Regulation, Neoplastic; D020868:Gene Silencing; D017930:Genes, Reporter; D006801:Humans; D047468:Immunoprecipitation; D007249:Inflammation; D047908:Intracellular Signaling Peptides and Proteins / Q000378:metabolism*; D051379:Mice; D035683:MicroRNAs / Q000235:genetics* / Q000502:physiology*; D009154:Mutation; D016328:NF-kappa B / Q000378:metabolism; D028861:Nuclear Pore Complex Proteins / Q000378:metabolism; D009687:Nuclear Proteins / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000378:metabolism; D017422:Sequence Analysis, DNA; D000072598:Tumor Necrosis Factor alpha-Induced Protein 3
chemical_list: D020413:3' Untranslated Regions; C119724:AGFG1 protein, human; D004268:DNA-Binding Proteins; D051959:ELAV Proteins; D047908:Intracellular Signaling Peptides and Proteins; C532045:MIRN29a microRNA, human; D035683:MicroRNAs; D016328:NF-kappa B; D028861:Nuclear Pore Complex Proteins; D009687:Nuclear Proteins; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; C076529:TNFAIP3 protein, human; D000072598:Tumor Necrosis Factor alpha-Induced Protein 3
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