title: MicroRNA-106b regulates the tumor suppressor RUNX3 in laryngeal carcinoma cells.
abstract: Our study focuses on a set of laryngeal tumors that show reduced RUNX3 expression in the absence of transcriptional silencing of tumor suppressor gene RUNX3 by aberrant methylation of CpG islands. We report that the loss of expression of RUNX3 correlates with up-regulation of miR-106b in human laryngeal carcinoma tissue. The downregulation of RUNX3 is mediated by miR-106b through binding of its 3'UTR. Moreover, miR-106b can promote the proliferation and invasion of laryngeal carcinoma cells by directly targeting RUNX3, and RUXN3 knockdown can abolish this phenotype. These results shed a new insight into the mechanism of miRNA regulation in laryngeal carcinoma.
authors.affiliation: Department of Otolaryngology, Head and Neck Surgery, The First Hospital, Shanxi Medical University, Taiyuan, Shanxi, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Xu
authors.forename: Kai
authors.initials: K
authors.lastname: Wang
authors.forename: Wei
authors.initials: W
authors.lastname: Gao
authors.forename: Chunming
authors.initials: C
authors.lastname: Zhang
authors.forename: Fuhui
authors.initials: F
authors.lastname: Huang
authors.forename: Shuxin
authors.initials: S
authors.lastname: Wen
authors.forename: Binquan
authors.initials: B
authors.lastname: Wang
mesh_terms: D000818:Animals; D002455:Cell Division / Q000502:physiology; D050677:Core Binding Factor Alpha 3 Subunit / Q000235:genetics*; D019175:DNA Methylation; D015536:Down-Regulation; D015972:Gene Expression Regulation, Neoplastic / Q000502:physiology*; D016147:Genes, Tumor Suppressor; D006801:Humans; D007822:Laryngeal Neoplasms / Q000235:genetics* / Q000473:pathology; D051379:Mice; D008819:Mice, Nude; D035683:MicroRNAs / Q000502:physiology*; D009361:Neoplasm Invasiveness; D014407:Tumor Cells, Cultured
chemical_list: D050677:Core Binding Factor Alpha 3 Subunit; C542541:MIRN106 microRNA, human; D035683:MicroRNAs; C493729:Runx3 protein, human
keywords: Invasion; Laryngeal carcinoma; Proliferation; RUNX3; miR-106b