title: MicroRNA-214 protects cardiac myocytes against H2O2-induced injury.
abstract: Reactive oxygen species (ROS)-induced cardiac myocyte injury resulting from changes in the expression levels of multiple genes plays a critical role in the pathogenesis of numerous heart diseases. The purpose of this study was to determine the potential roles of microRNA-214 (miR-214) in hydrogen peroxide (H2O2)-mediated gene regulation in cardiac myocytes. In this study, we used quantitative real-time RT-PCR (qRT-PCR) to demonstrate that miR-214 was upregulated in cardiac myocytes after treatment with H2O2. We transfected cells with pre-miR-214 to upregulate miR-214 expression and transfected cells with a miR-214 inhibitor (anti-miR-214) to downregulate miR-214 expression. H2O2-induced cardiac cell apoptosis was detected by flow cytometry. The level of apoptosis was increased by the miR-214 inhibitor and decreased by pre-miR-214. Therefore, we believe that miR-214 plays a positive role in H2O2-induced cardiac cell apoptosis. Phosphatase and tensin homolog deleted on chromosome 10 (PTEN) is constitutively active and is considered to be the primary downregulator of the pro-oncogenic PI3K/Akt pathway. Western blot analysis revealed that the expression of the PTEN protein in cardiac myocytes decreased after H2O2 induction. Anti-miR-214 increased PTEN protein expression level, in contrast, pre-miR-214 decreased the PTEN protein expression level in cultured cardiac myocytes. These results indicate that PTEN is regulated by miR-214 and serves as an important target of miR-214 in cardiac myocytes. In conclusion, miR-214 is sensitive to H2O2 stimulation, and miR-214 protects cardiac myocytes against H2O2-induced injury via one of its targets, PTEN.
authors.affiliation: Department of ICU, The Third Hospital of Hebei Medical University, Shijiazhuang City, Hebei Province, P.R. China.
authors.forename: Guangwei
authors.initials: G
authors.lastname: Lv
authors.forename: Suxia
authors.initials: S
authors.lastname: Shao
authors.forename: Hua
authors.initials: H
authors.lastname: Dong
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Bian
authors.forename: Xingwei
authors.initials: X
authors.lastname: Yang
authors.forename: Shimin
authors.initials: S
authors.lastname: Dong
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects / Q000235:genetics; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D005786:Gene Expression Regulation / Q000187:drug effects; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D035683:MicroRNAs / Q000502:physiology*; D032383:Myocytes, Cardiac / Q000187:drug effects / Q000502:physiology*; D051059:PTEN Phosphohydrolase / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D035683:MicroRNAs; C559947:Mirn214 microRNA, rat; D006861:Hydrogen Peroxide; D051059:PTEN Phosphohydrolase; C487216:Pten protein, rat
keywords: APOPTOSIS; CARDIAC MYOCYTES; H2O2; MicroRNA-214; PHOSPHATASE AND TENSIN HOMOLOG (PTEN)