title: [Effect of Kozak sequence on mice DNA vaccine immunization of Staphylococcus aureus adhesion fibronectin-binding protein FnBPA-A].
abstract: Fibronectin-binding protein (FnBPA) is a protein that expresses on cell surface of Staphylococcus aureus during early stage of infection. FnBPA was capable of promoting Staphylococcus aureus to invade cells and was viewed as a potential immune target. Based on the FnBPA-A gene two recombinant expression vectors with or without Kozak sequence were constructed. After identified and confirmed by restriction enzyme digestion and sequencing they were used to immunize C57BL/6 mice. Then induced antibody titer, T lymphocyte proliferative response and experiment mice challenge test were measured. Our result indicates that humoral immune responses and challenge experiment induced by recombinant DNA with Kozak sequence were better than those without Kozak sequence (P < 0.05). For T lymphocyte proliferative response the induced effect of recombinant DNA with Kozak sequence was higher than that without Kozak sequence, but there was no significant difference (P > 0.05). We conclude that Kozak sequence could play an important role in immune response induced by FnBPA-A recombinant DNA.
authors.affiliation: College of Veterinary Medicine, Xinjiang Agricultural University, Urumqi 830052, Xinjiang, China. 2006au@163.com
authors.forename: Yan
authors.initials: Y
authors.lastname: Su
authors.forename: Shimin
authors.initials: S
authors.lastname: Wang
authors.forename: Jungao
authors.initials: J
authors.lastname: Shao
authors.forename: Baojiang
authors.initials: B
authors.lastname: Zhang
authors.forename: Haina
authors.initials: H
authors.lastname: Wei
mesh_terms: D018829:Adhesins, Bacterial / Q000235:genetics / Q000276:immunology*; D000818:Animals; D007114:Immunization; D051379:Mice; D008810:Mice, Inbred C57BL; D011994:Recombinant Proteins / Q000276:immunology; D013211:Staphylococcus aureus / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*; D019444:Vaccines, DNA / Q000235:genetics* / Q000276:immunology
chemical_list: D018829:Adhesins, Bacterial; D011994:Recombinant Proteins; D019444:Vaccines, DNA; C078635:fibronectin-binding proteins, bacterial