title: [Effects of soluble adult worm antigen and soluble egg antigen of Schistosoma japonicum on differentiation of CD4+ T cells of mice].
abstract: OBJECTIVE
  To investigate and compare the different effects of soluble adult worm antigen (SWA) and soluble egg antigen (SEA) of Schistosoma japonicum on the differentiation of the splenocytes and CD4+ T cells of mice.
  
  
  METHODS
  The splenocytes and CD4+ T cells were prepared from the spleens of mice immunized with SWA or SEA, or the splenocytes of normal mice were harvested and stimulated with SWA or SEA in vitro. Then, the proportions of IFN-gamma and IL-4-producing cells in splenocytes, and the proportions of Th1 and Th2 cells in CD4+ T cells were determined by FACS, respectively.
  
  
  RESULTS
  Compared to the SWA stimulation group, the higher proportions of IL-4-producing cells in splenocytes and of Th1 cells in CD4+ T cells were observed under the SEA stimulation group (P < 0.05). Whereas SWA induced the significantly higher proportions of IFN-gamma producing cells in splenocytes and of Th2 cells in CD4+ T cells than those in the SEA stimulation group (P < 0.05).
  
  
  CONCLUSIONS
  The significantly higher levels of Th1 cells are only observed under SWA induction, however, the differentiation of Th2 cells in response to SEA stimulation is significantly more than that in response to SWA stimulation.
authors.affiliation: Department of Pathogen Biology, Nanjing Medical University, Nanjing 210029, China.
authors.forename: Xiao-Wei
authors.initials: XW
authors.lastname: Yang
authors.forename: Cui
authors.initials: C
authors.lastname: Zhang
authors.forename: Xiao-Xiao
authors.initials: XX
authors.lastname: Dong
authors.forename: Yong
authors.initials: Y
authors.lastname: Li
authors.forename: Zhi-Peng
authors.initials: ZP
authors.lastname: Xu
authors.forename: Wei-Wei
authors.initials: WW
authors.lastname: Zhang
authors.forename: Wen-Jun
authors.initials: WJ
authors.lastname: Kong
authors.forename: Xue
authors.initials: X
authors.lastname: Xue
authors.forename: Xiao-Jun
authors.initials: XJ
authors.lastname: Chen
authors.forename: Ji-Feng
authors.initials: JF
authors.lastname: Zhu
authors.forename: Sha
authors.initials: S
authors.lastname: Zhou
authors.forename: Lei
authors.initials: L
authors.lastname: He
authors.forename: Feng
authors.initials: F
authors.lastname: Liu
authors.forename: Chuan
authors.initials: C
authors.lastname: Su
mesh_terms: D000818:Animals; D000947:Antigens, Helminth / Q000276:immunology*; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology* / Q000469:parasitology; D002454:Cell Differentiation / Q000276:immunology; D005260:Female; D007371:Interferon-gamma / Q000276:immunology; D015847:Interleukin-4 / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D010063:Ovum / Q000276:immunology*; D012549:Schistosoma japonicum / Q000276:immunology*; D012554:Schistosomiasis japonica / Q000276:immunology / Q000469:parasitology; D018417:Th1 Cells / Q000276:immunology / Q000469:parasitology; D018418:Th2 Cells / Q000276:immunology / Q000469:parasitology
chemical_list: D000947:Antigens, Helminth; D015847:Interleukin-4; D007371:Interferon-gamma