title: Bench-scale bioethanol production from eucalyptus by high solid saccharification and glucose/xylose fermentation method.
abstract: In the bioethanol production process, high solid saccharification and glucose/xylose co-fermentation are important technologies for obtaining increased ethanol concentrations; however, bench-scale studies using combinations of these methods are limited. In this study, we hydrolyzed high solid concentration of milled eucalyptus using commercial enzymes and obtained 138.4 g/L total monomeric sugar concentration. These sugars were fermented to 53.5 g/L of ethanol by a xylose-utilizing recombinant Saccharomyces cerevisiae strain, MA-R4. These experiments were performed in bench scale (using 50 L scale solid mixer and 70 L scale fermenter). The results obtained in this study were comparable to our previous results in laboratory scale, indicating that we successfully achieved an efficient high solid saccharification and glucose/xylose co-fermentation system in bench scale.
authors.affiliation: Biomass Refinery Research Center, National Institute of Advanced Industrial Science and Technology (AIST), 3-11-32 Kagamiyama, Higashi-Hiroshima, Hiroshima, 739-0046, Japan, tatsuya.fujii@aist.go.jp.
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Fujii
authors.forename: Katsuji
authors.initials: K
authors.lastname: Murakami
authors.forename: Takashi
authors.initials: T
authors.lastname: Endo
authors.forename: Shinji
authors.initials: S
authors.lastname: Fujimoto
authors.forename: Tomoaki
authors.initials: T
authors.lastname: Minowa
authors.forename: Akinori
authors.initials: A
authors.lastname: Matsushika
authors.forename: Shinichi
authors.initials: S
authors.lastname: Yano
authors.forename: Shigeki
authors.initials: S
authors.lastname: Sawayama
mesh_terms: D000431:Ethanol / Q000378:metabolism*; D005052:Eucalyptus / Q000737:chemistry*; D005285:Fermentation / Q000502:physiology*; D005947:Glucose / Q000737:chemistry / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000254:growth & development*; D014994:Xylose / Q000737:chemistry / Q000378:metabolism
chemical_list: D000431:Ethanol; D014994:Xylose; D005947:Glucose
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