title: Fused (Stk36) is a ciliary protein required for central pair assembly and motile cilia orientation in the mammalian oviduct.
abstract: BACKGROUND
  Motile cilia on the inner lining of the oviductal epithelium play a central role in ovum transport toward the uterus and subsequent fertilization by sperm. While the basic ultrastructure of 9+2 motile cilia (nine peripheral microtubule doublets surrounding a central pair) has been characterized, many important steps of ciliogenesis remain poorly understood.
  
  
  RESULTS
  Our previous studies on mammalian Fused (Fu) (Stk36), a putative serine-threonine kinase, reveal a critical function of Fu in central pair construction and cilia orientation of motile cilia that line the tracheal and ependymal epithelia. These findings identify a novel regulatory component for these processes. In this study, we show that Fu is expressed in the multi-ciliated oviductal epithelium in several vertebrates, suggesting a conserved function of Fu in the oviduct. In support of this, analysis of Fu-deficient mouse oviducts uncovers a similar role of Fu in central pair construction and cilia orientation. We also demonstrate that Fu localizes to motile cilia and physically associates with kinesin Kif27 located at the cilium base and known central pair components Spag16 and Pcdp1.
  
  
  CONCLUSIONS
  Our results delineate a novel pathway for central pair apparatus assembly and add important insight to the biogenesis and function of oviductal motile cilia.
authors.affiliation: Cardiovascular Research Institute, University of California, San Francisco, California.
authors.forename: Yoko Inès
authors.initials: YI
authors.lastname: Nozawa
authors.forename: Erica
authors.initials: E
authors.lastname: Yao
authors.forename: Chuwen
authors.initials: C
authors.lastname: Lin
authors.forename: Jehn-Hsiahn
authors.initials: JH
authors.lastname: Yang
authors.forename: Christopher W
authors.initials: CW
authors.lastname: Wilson
authors.forename: Rhodora
authors.initials: R
authors.lastname: Gacayan
authors.forename: Pao-Tien
authors.initials: PT
authors.lastname: Chuang
mesh_terms: D000818:Animals; D002460:Cell Line; D002923:Cilia / Q000378:metabolism* / Q000648:ultrastructure; D005260:Female; D006801:Humans; D017403:In Situ Hybridization; D008322:Mammals / Q000196:embryology / Q000378:metabolism; D051379:Mice; D008817:Mice, Mutant Strains; D046529:Microscopy, Electron, Transmission; D010057:Oviducts / Q000196:embryology* / Q000378:metabolism*; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism*
chemical_list: D017346:Protein Serine-Threonine Kinases
keywords: Fu (Stk36); Kif27; central pair; motile cilia; oviduct; primary ciliary dyskinesia