title: Impedimetric detection of in situ interaction between anti-cancer drug bleomycin and DNA.
abstract: Surface confined interaction of anti-cancer drug bleomycin (BLM) with nucleic acids: single stranded and double stranded DNA was investigated herein by using electrochemical impedance spectroscopy (EIS) technique in combination with a graphite sensor technology. The experimental conditions were optimized: such as, dsDNA concentration, BLM concentration and interaction time. The main features of impedimetric DNA biosensor, such as its detection limit and the repeatability, were also discussed. The in situ interaction of BLM with dsDNA was also tested impedimetrically in the absence or presence of other chemotherapeutic agents, such as mitomycin C (MC) and cis-platin (cis-DDP) for testing the selectivity.
authors.affiliation: Ege University, Faculty of Pharmacy, Analytical Chemistry Dept., Bornova, 35100 Izmir, Turkey. Electronic address: arzum.erdem@ege.edu.tr.
authors.forename: Arzum
authors.initials: A
authors.lastname: Erdem
authors.forename: Gulsah
authors.initials: G
authors.lastname: Congur
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry* / Q000378:metabolism; D015374:Biosensing Techniques; D001761:Bleomycin / Q000737:chemistry* / Q000378:metabolism; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D058266:Dielectric Spectroscopy; D017097:Electric Impedance; D004563:Electrochemistry
chemical_list: D000970:Antineoplastic Agents; D001761:Bleomycin; D004247:DNA
keywords: Bleomycin; Cis-platin; Drug–DNA interactions; Electrochemical impedance spectroscopy; Mitomycin C