title: Genetic polymorphisms in Cytochrome P 4501B1 and susceptibility to idiopathic preterm labor in North Indian population.
abstract: OBJECTIVE
  The etiology of preterm labor (PTL) is still unknown, but it may be related to a possible genetic predisposition together with involvement of environmental factors. We investigated the relation between PTL and polymorphisms in Cytochrome P4501B1 (CYP1B1) gene, which is involved in the metabolism of a wide range of environmental toxins and hormones.
  
  
  METHODS
  Three hundred (n=300) cases of PTL and equal number of subjects of full term labor (FTL), after excluding all the known risk factors for PTL were included in the study. A two step allele specific PCR was performed for polymorphic analysis of CYP1B1 gene.
  
  
  RESULTS
  The homozygous variant genotype of CYP1B1*2 (OR=2.97, 95%CI=1.08-8.08, p=0.033) and heterozygous variant of CYP1B1*3 (OR=2.57, 95%CI=1.88-3.63, p=0.001), and CYP1B1*7 (OR=2.59, 95%CI=1.85-3.62, p=0.001) were found to be significantly higher in PTL cases as compared to FTL.
  
  
  CONCLUSIONS
  The present study demonstrates the possible association of homozygous variant of CYP1B1*2 and heterozygous variant of CYP1B1*3 and CYP1B1*7 genes with the increased risk of PTL.
authors.affiliation: Environmental Biochemistry and Molecular Biology Laboratory, Department of Biochemistry, University College of Medical Sciences & G.T.B. Hospital (University of Delhi), Dilshad Garden, Delhi 110 095, India.
authors.forename: Md
authors.initials: M
authors.lastname: Mustafa
authors.forename: Tusha
authors.initials: T
authors.lastname: Sharma
authors.forename: B D
authors.initials: BD
authors.lastname: Banerjee
authors.forename: M
authors.initials: M
authors.lastname: Phil
authors.forename: Rafat S
authors.initials: RS
authors.lastname: Ahmed
authors.forename: A K
authors.initials: AK
authors.lastname: Tripathi
authors.forename: Kiran
authors.initials: K
authors.lastname: Guleria
mesh_terms: D000328:Adult; D001189:Aryl Hydrocarbon Hydroxylases / Q000235:genetics*; D016022:Case-Control Studies; D065633:Cytochrome P-450 CYP1B1; D005260:Female; D020022:Genetic Predisposition to Disease; D006579:Heterozygote; D006720:Homozygote; D006801:Humans; D007194:India; D008423:Maternal Age; D007752:Obstetric Labor, Premature / Q000235:genetics*; D011110:Polymorphism, Genetic; D011247:Pregnancy; D044465:White People / Q000235:genetics
chemical_list: D001189:Aryl Hydrocarbon Hydroxylases; C585496:CYP1B1 protein, human; D065633:Cytochrome P-450 CYP1B1
keywords: Allele specific PCR; Genetic susceptibility; Heterozygous variants; Homozygous variants; Preterm labor; Single nucleotide polymorphism