title: Tracking the engraftment and regenerative capabilities of transplanted lung stem cells using fluorescent nanodiamonds.
abstract: Lung stem/progenitor cells are potentially useful for regenerative therapy, for example in repairing damaged or lost lung tissue in patients. Several optical imaging methods and probes have been used to track how stem cells incorporate and regenerate themselves in vivo over time. However, these approaches are limited by photobleaching, toxicity and interference from background tissue autofluorescence. Here we show that fluorescent nanodiamonds, in combination with fluorescence-activated cell sorting, fluorescence lifetime imaging microscopy and immunostaining, can identify transplanted CD45(-)CD54(+)CD157(+) lung stem/progenitor cells in vivo, and track their engraftment and regenerative capabilities with single-cell resolution. Fluorescent nanodiamond labelling did not eliminate the cells' properties of self-renewal and differentiation into type I and type II pneumocytes. Time-gated fluorescence imaging of tissue sections of naphthalene-injured mice indicates that the fluorescent nanodiamond-labelled lung stem/progenitor cells preferentially reside at terminal bronchioles of the lungs for 7 days after intravenous transplantation.
authors.affiliation: Institute of Biochemistry and Molecular Biology, Program in Molecular Medicine, School of Life Sciences, National Yang-Ming University, Taipei 112, Taiwan.
authors.forename: Tsai-Jung
authors.initials: TJ
authors.lastname: Wu
authors.forename: Yan-Kai
authors.initials: YK
authors.lastname: Tzeng
authors.forename: Wei-Wei
authors.initials: WW
authors.lastname: Chang
authors.forename: Chi-An
authors.initials: CA
authors.lastname: Cheng
authors.forename: Yung
authors.initials: Y
authors.lastname: Kuo
authors.forename: Chin-Hsiang
authors.initials: CH
authors.lastname: Chien
authors.forename: Huan-Cheng
authors.initials: HC
authors.lastname: Chang
authors.forename: John
authors.initials: J
authors.lastname: Yu
mesh_terms: D064591:Allografts; D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D002454:Cell Differentiation; D005456:Fluorescent Dyes / Q000494:pharmacology*; D008168:Lung / Q000166:cytology* / Q000378:metabolism; D051379:Mice; D008813:Mice, Inbred ICR; D008856:Microscopy, Fluorescence / Q000379:methods; D058612:Nanodiamonds; D044968:Regenerative Medicine / Q000379:methods; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism
chemical_list: D015703:Antigens, CD; D005456:Fluorescent Dyes; D058612:Nanodiamonds
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