title: Extracellular proteome analysis of Leptospira interrogans serovar Lai.
abstract: Abstract Leptospirosis is one of the most important zoonoses. Leptospira interrogans serovar Lai is a pathogenic spirochete that is responsible for leptospirosis. Extracellular proteins play an important role in the pathogenicity of this bacterium. In this study, L. interrogans serovar Lai was grown in protein-free medium; the supernatant was collected and subsequently analyzed as the extracellular proteome. A total of 66 proteins with more than two unique peptides were detected by MS/MS, and 33 of these were predicted to be extracellular proteins by a combination of bioinformatics analyses, including Psortb, cello, SoSuiGramN and SignalP. Comparisons of the transcriptional levels of these 33 genes between in vivo and in vitro conditions revealed that 15 genes were upregulated and two genes were downregulated in vivo compared to in vitro. A BLAST search for the components of secretion system at the genomic and proteomic levels revealed the presence of the complete type I secretion system and type II secretion system in this strain. Moreover, this strain also exhibits complete Sec translocase and Tat translocase systems. The extracellular proteome analysis of L. interrogans will supplement the previously generated whole proteome data and provide more information for studying the functions of specific proteins in the infection process and for selecting candidate molecules for vaccines or diagnostic tools for leptospirosis.
authors.affiliation: 1 Department of Medical Microbiology and Parasitology, Institutes of Medical Sciences, Shanghai Jiao Tong University School of Medicine , Shanghai, China .
authors.forename: Lingbing
authors.initials: L
authors.lastname: Zeng
authors.forename: Yunyi
authors.initials: Y
authors.lastname: Zhang
authors.forename: Yongzhang
authors.initials: Y
authors.lastname: Zhu
authors.forename: Haidi
authors.initials: H
authors.lastname: Yin
authors.forename: Xuran
authors.initials: X
authors.lastname: Zhuang
authors.forename: Weinan
authors.initials: W
authors.lastname: Zhu
authors.forename: Xiaokui
authors.initials: X
authors.lastname: Guo
authors.forename: Jinhong
authors.initials: J
authors.lastname: Qin
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D058947:Bacterial Secretion Systems; D003470:Culture Media; D015964:Gene Expression Regulation, Bacterial; D007921:Leptospira interrogans / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D008074:Lipoproteins / Q000235:genetics / Q000378:metabolism; D020543:Proteome / Q000235:genetics / Q000378:metabolism*; D055571:Secretory Pathway; D053719:Tandem Mass Spectrometry; D059467:Transcriptome; D037521:Virulence Factors / Q000235:genetics / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D058947:Bacterial Secretion Systems; D003470:Culture Media; D008074:Lipoproteins; D020543:Proteome; D037521:Virulence Factors
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