title: Preparation and structural elucidation of a glucomannogalactan from marine fungus Penicillium commune.
abstract: The coral-associated fungus Penicillium commune produces an extracellular polysaccharide, FP2-1, when grown in potato dextrose-agar medium. FP2-1 was isolated from the fermented broth using anion-exchange and size-exclusion chromatography, and its structure was elucidated by chemical and spectroscopic analyses, including detailed nuclear magnetic resonance spectroscopy. The results showed that FP2-1 was a glucomannogalactan with a molar ratio of galactose, mannose and glucose of 3.9:1.9:1.0. Structure of FP2-1 may be represented, at an average, as a backbone of (1→2)-linked α-mannopyranose with the every second residue substituted at position 6 by a pentasaccharide branch. The branches consist of four (1→6)-linked β-galactofuranose residues with terminal α-glucopyranose residue attached to the last galactofuranose residue at position 2. FP2-1 was a novel galactofuranose-containing extracellular polysaccharide differing from previously described extracellular polysaccharides.
authors.affiliation: Key Laboratory of Marine Drugs, Ministry of Education, School of Medicine and Pharmacy, Ocean University of China, 5 Yushan Road, Qingdao 266003, People's Republic of China.
authors.forename: Yanli
authors.initials: Y
authors.lastname: Chen
authors.forename: Wenjun
authors.initials: W
authors.lastname: Mao
authors.forename: Junfeng
authors.initials: J
authors.lastname: Wang
authors.forename: Weiming
authors.initials: W
authors.lastname: Zhu
authors.forename: Chunqi
authors.initials: C
authors.lastname: Zhao
authors.forename: Na
authors.initials: N
authors.lastname: Li
authors.forename: Chunyan
authors.initials: C
authors.lastname: Wang
authors.forename: Mengxia
authors.initials: M
authors.lastname: Yan
authors.forename: Tao
authors.initials: T
authors.lastname: Guo
authors.forename: Xue
authors.initials: X
authors.lastname: Liu
mesh_terms: D000818:Animals; D037421:Anthozoa / Q000382:microbiology; D059001:Aquatic Organisms / Q000737:chemistry*; D002850:Chromatography, Gel; D005110:Extracellular Space / Q000737:chemistry; D005685:Galactans / Q000096:biosynthesis* / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008745:Methylation; D010407:Penicillium / Q000737:chemistry*; D013055:Spectrophotometry, Infrared; D013402:Sugar Alcohols / Q000378:metabolism
chemical_list: D005685:Galactans; D013402:Sugar Alcohols
keywords: Coral-associated fungus; Extracellular polysaccharide; Penicillium commune; Preparation; Structure