title: Live cell imaging of cytoplasmic and nuclear Ca2+ dynamics in Arabidopsis roots.
abstract: Approaches for Ca(2+) measurement require data recording with high spatiotemporal resolution. This is achieved by combined usage of locally targeted genetically encoded calcium indicators (GECIs) and confocal laser scanning microscopy (CLSM). This protocol provides instructions for setting up a CLSM-based experiment to measure cytosolic and nuclear Ca(2+) dynamics in Arabidopsis lines expressing Yellow Cameleon Ca(2+) indicators.
authors.affiliation: Department of Developmental Biology, Centre for Organismal Studies, University of Heidelberg, 69120 Heidelberg, Germany. melanie.krebs@cos.uni-heidelberg.de
authors.forename: Melanie
authors.initials: M
authors.lastname: Krebs
authors.forename: Karin
authors.initials: K
authors.lastname: Schumacher
mesh_terms: D017360:Arabidopsis / Q000166:cytology; D002118:Calcium / Q000032:analysis*; D002135:Calcium-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D002413:Cations, Divalent / Q000032:analysis; D002467:Cell Nucleus / Q000737:chemistry*; D003584:Cytological Techniques / Q000379:methods*; D003593:Cytoplasm / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000378:metabolism*; D018613:Microscopy, Confocal / Q000379:methods*; D018517:Plant Roots / Q000166:cytology; D013194:Staining and Labeling / Q000379:methods; D019076:Transgenes
chemical_list: D002135:Calcium-Binding Proteins; D002413:Cations, Divalent; D005456:Fluorescent Dyes; C495639:yellow cameleon; D002118:Calcium