title: 3D culture of adipose-tissue-derived stem cells mainly leads to chondrogenesis in poly(ethylene glycol)-poly(L-alanine) diblock copolymer thermogel.
abstract: Poly(ethylene glycol)-b-poly(L-alanine) (PEG-L-PA)s with L-PA molecular weights of 620, 1100, and 2480 Da and a fixed molecular weight of PEG at 5000 Da were synthesized to compare the thermosensitive behavior, and to investigate their potential as a three-dimensional (3D) culture matrix of adipose-tissue-derived stem cells (ADSCs). The sol-to-gel transition temperature and the concentration ranges where the transition was observed decreased as the L-PA molecular weight increased. ADSCs were cultured in the 3D matrixes of in situ formed PEG-L-PA hydrogels, which were produced by increasing the temperature of cell-suspended PEG-L-PA aqueous solutions. The spherical morphology was maintained in the PEG-L-PA hydrogel, while the cells underwent fibroblastic morphological changes in the Matrigel over 14 days of incubation. ADSCs exhibited high expression of type II collagen in the PEG-L-PA thermogel. In addition, they also moderately expressed the biomarker of myogenic differentiation factor 1 as the same mesodermal lineages, as well as the type III β-tubulin as a cross-differentiation biomarker. Similar to the in vitro study, the ADSCs predominantly exhibited chondrogenic biomarkers in the in vivo study. The study demonstrates that the polypeptide thermogel of PEG-L-PA is promising as a 3D culture matrix of ADSCs and as an injectable tissue engineering biomaterial.
authors.affiliation: Department of Chemistry and Nano Science, Ewha Global Top 5 Research Program, Ewha Womans University, 52 Ewhayeodae-gil, Seodaemun-gu, Seoul 120-750, Korea.
authors.forename: Bora
authors.initials: B
authors.lastname: Yeon
authors.forename: Min Hee
authors.initials: MH
authors.lastname: Park
authors.forename: Hyo Jung
authors.initials: HJ
authors.lastname: Moon
authors.forename: Seung-Jin
authors.initials: SJ
authors.lastname: Kim
authors.forename: Young Woo
authors.initials: YW
authors.lastname: Cheon
authors.forename: Byeongmoon
authors.initials: B
authors.lastname: Jeong
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000293:Adolescent; D053687:Adult Stem Cells / Q000502:physiology* / Q000637:transplantation; D000818:Animals; D000943:Antigens, Differentiation / Q000378:metabolism; D001672:Biocompatible Materials / Q000737:chemistry; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D049109:Cell Proliferation; D002470:Cell Survival; D002478:Cells, Cultured; D020219:Chondrogenesis; D015870:Gene Expression; D006801:Humans; D020100:Hydrogels / Q000737:chemistry; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010316:Particle Size; D010455:Peptides / Q000737:chemistry*; D044367:Phase Transition; D011092:Polyethylene Glycols / Q000737:chemistry*; D044968:Regenerative Medicine; D012996:Solutions; D023822:Tissue Engineering
chemical_list: D000943:Antigens, Differentiation; D001672:Biocompatible Materials; D020100:Hydrogels; D010455:Peptides; D012996:Solutions; C019529:polyalanine; D011092:Polyethylene Glycols