title: Hypoxia promotes cell proliferation by modulating E2F1 in chicken pulmonary arterial smooth muscle cells.
abstract: In this study, we sought to investigate the expression of the transcription factor E2F1 in chicken pulmonary arterial smooth muscle cells upon hypoxia exposure, as well as the role that E2F1 played in the regulation of cell proliferation. Isolated chicken pulmonary arterial smooth muscle cells were subjected to hypoxia or normoxia for indicated time points. Cell viability, DNA synthesis, cell cycle profile, and expression of E2F1 were analyzed. The results showed that hypoxia promoted cell proliferation and DNA synthesis which was accompanied by an increased S phase entry and upregulation of E2F1 at mRNA and protein levels. Using siRNA technology, we demonstrated that gene inactivation of endogenous E2F1 abolished hypoxia-induced cell proliferation, DNA synthesis, and S phase entry compared with negative siRNA transfected cells. These results suggest that hypoxia-induced proliferation is mediated by inducing E2F1 in chicken pulmonary arterial smooth muscle cells.
authors.affiliation: State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing 100193, P,R, China. cauvet@163.com.
authors.forename: Ying
authors.initials: Y
authors.lastname: Yang
authors.forename: Feng
authors.initials: F
authors.lastname: Sun
authors.forename: Chen
authors.initials: C
authors.lastname: Zhang
authors.forename: Hao
authors.initials: H
authors.lastname: Wang
authors.forename: Guoyao
authors.initials: G
authors.lastname: Wu
authors.forename: Zhenlong
authors.initials: Z
authors.lastname: Wu
references.citation: Paediatr Respir Rev. 2006 Mar;7(1):36-44
references.pmid: 16473815
references.citation: Anim Sci J. 2010 Aug 1;81(4):494-500
references.pmid: 20662820
references.citation: Lancet. 2003 May 3;361(9368):1533-44
references.pmid: 12737878
references.citation: Br Poult Sci. 2012;53(5):608-15
references.pmid: 23281754
references.citation: Am J Respir Cell Mol Biol. 2001 Nov;25(5):652-8
references.pmid: 11713109
references.citation: Lipids. 1998 Feb;33(2):181-90
references.pmid: 9507240
references.citation: Nucleic Acids Res. 2002 Apr 15;30(8):1859-67
references.pmid: 11937641
references.citation: FEBS Lett. 2002 Oct 2;529(1):122-5
references.pmid: 12354623
references.citation: Biochim Biophys Acta. 2002 Jun 21;1602(2):131-50
references.pmid: 12020800
references.citation: Int J Biochem Cell Biol. 2009 Dec;41(12):2389-97
references.pmid: 19539777
references.citation: Curr Mol Pharmacol. 2009 Jun;2(2):149-60
references.pmid: 20021455
references.citation: Nat Cell Biol. 2001 Jun;3(6):552-8
references.pmid: 11389439
references.citation: Eur Respir J. 2007 Aug;30(2):364-72
references.pmid: 17666559
references.citation: Trends Biochem Sci. 2004 Aug;29(8):409-17
references.pmid: 15362224
references.citation: Histochemistry. 1980;69(2):113-30
references.pmid: 7007294
references.citation: Poult Sci. 2013 Jan;92(1):64-83
references.pmid: 23243232
references.citation: Eur J Pharmacol. 2007 Apr 30;561(1-3):137-43
references.pmid: 17320074
references.citation: Am J Respir Cell Mol Biol. 2012 Mar;46(3):323-30
references.pmid: 21997485
references.citation: Avian Pathol. 2008 Apr;37(2):117-26
references.pmid: 18393088
references.citation: Anesth Analg. 2007 Oct;105(4):1034-41, table of contents
references.pmid: 17898384
references.citation: N Engl J Med. 2004 Sep 30;351(14):1425-36
references.pmid: 15459304
references.citation: Pharmacol Ther. 2001 Oct;92(1):1-20
references.pmid: 11750034
references.citation: Circ Res. 2006 Sep 29;99(7):675-91
references.pmid: 17008597
references.citation: Annu Rev Physiol. 1997;59:89-144
references.pmid: 9074758
references.citation: Am J Respir Cell Mol Biol. 2011 Nov;45(5):923-30
references.pmid: 21454803
references.citation: J Appl Physiol (1985). 2001 Sep;91(3):1421-30
references.pmid: 11509544
references.citation: J Clin Invest. 1999 Mar;103(5):691-6
references.pmid: 10074486
references.citation: Circ Res. 1999 Feb 19;84(3):329-36
references.pmid: 10024307
references.citation: Eur J Pharmacol. 2010 Dec 15;649(1-3):242-8
references.pmid: 20854807