title: Overexpression of the auxin binding protein1 modulates PIN-dependent auxin transport in tobacco cells.
abstract: BACKGROUND
  Auxin binding protein 1 (ABP1) is a putative auxin receptor and its function is indispensable for plant growth and development. ABP1 has been shown to be involved in auxin-dependent regulation of cell division and expansion, in plasma-membrane-related processes such as changes in transmembrane potential, and in the regulation of clathrin-dependent endocytosis. However, the ABP1-regulated downstream pathway remains elusive.
  
  
  RESULTS
  Using auxin transport assays and quantitative analysis of cellular morphology we show that ABP1 regulates auxin efflux from tobacco BY-2 cells. The overexpression of ABP1can counterbalance increased auxin efflux and auxin starvation phenotypes caused by the overexpression of PIN auxin efflux carrier. Relevant mechanism involves the ABP1-controlled vesicle trafficking processes, including positive regulation of endocytosis of PIN auxin efflux carriers, as indicated by fluorescence recovery after photobleaching (FRAP) and pharmacological manipulations.
  
  
  CONCLUSIONS
  The findings indicate the involvement of ABP1 in control of rate of auxin transport across plasma membrane emphasizing the role of ABP1 in regulation of PIN activity at the plasma membrane, and highlighting the relevance of ABP1 for the formation of developmentally important, PIN-dependent auxin gradients.
authors.affiliation: Laboratory of Hormonal Regulations in Plants, Institute of Experimental Botany of the Academy of Sciences of the Czech Republic, Prague, Czech Republic, Czech Republic.
authors.forename: Milada
authors.initials: M
authors.lastname: Čovanová
authors.forename: Michael
authors.initials: M
authors.lastname: Sauer
authors.forename: Jan
authors.initials: J
authors.lastname: Rychtář
authors.forename: Jiří
authors.initials: J
authors.lastname: Friml
authors.forename: Jan
authors.initials: J
authors.lastname: Petrášek
authors.forename: Eva
authors.initials: E
authors.lastname: Zažímalová
mesh_terms: D017360:Arabidopsis / Q000166:cytology / Q000378:metabolism; D002460:Cell Line; D036681:Fluorescence Recovery After Photobleaching; D007210:Indoleacetic Acids / Q000378:metabolism*; D049990:Membrane Transport Modulators / Q000378:metabolism*; D018613:Microscopy, Confocal; D010940:Plant Proteins / Q000096:biosynthesis / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000096:biosynthesis* / Q000378:metabolism; D014026:Nicotiana / Q000166:cytology / Q000378:metabolism*
chemical_list: D007210:Indoleacetic Acids; D049990:Membrane Transport Modulators; D010940:Plant Proteins; D011956:Receptors, Cell Surface; C083846:auxin-binding protein 1; C083845:endoplasmic reticulum auxin-binding protein 4, Zea mays
references.citation: EMBO J. 1989 Sep;8(9):2453-61
references.pmid: 2555179
references.citation: Plant Cell. 1994 Dec;6(12):1941-53
references.pmid: 11536654
references.citation: J Biol Chem. 1985 Aug 15;260(17):9854-9
references.pmid: 2991267
references.citation: Proc Natl Acad Sci U S A. 1992 Aug 1;89(15):7208-12
references.pmid: 1323130
references.citation: Plant J. 2001 Dec;28(6):607-17
references.pmid: 11851907
references.citation: Cell. 2003 Nov 26;115(5):591-602
references.pmid: 14651850
references.citation: Cell Mol Life Sci. 2007 Jul;64(13):1621-37
references.pmid: 17458499
references.citation: Cell. 2010 Oct 1;143(1):111-21
references.pmid: 20887896
references.citation: Plant Physiol. 2002 Mar;128(3):935-50
references.pmid: 11891249
references.citation: Plant J. 2010 Mar;61(5):883-92
references.pmid: 20003134
references.citation: Proc Natl Acad Sci U S A. 1995 Apr 11;92(8):3425-9
references.pmid: 11607527
references.citation: Trends Plant Sci. 2010 Aug;15(8):436-46
references.pmid: 20605513
references.citation: Planta. 1972 Dec;107(4):325-40
references.pmid: 24477482
references.citation: Plant Physiol. 2012 Jun;159(2):632-41
references.pmid: 22492846
references.citation: Proc Natl Acad Sci U S A. 1989 Feb;86(3):891-5
references.pmid: 16594015
references.citation: Plant Physiol. 1993 Feb;101(2):595-606
references.pmid: 12231715
references.citation: Plant Physiol. 1977 Oct;60(4):585-91
references.pmid: 16660143
references.citation: Nature. 2005 Jun 30;435(7046):1251-6
references.pmid: 15988527
references.citation: J Biol Chem. 1985 Aug 15;260(17):9848-53
references.pmid: 2991266
references.citation: Curr Biol. 2007 Mar 20;17(6):520-7
references.pmid: 17306539
references.citation: Plant Physiol. 1985 Oct;79(2):568-70
references.pmid: 16664453
references.citation: Plant Physiol. 2003 Nov;133(3):1251-60
references.pmid: 14612587
references.citation: Plant Cell. 2008 Oct;20(10):2746-62
references.pmid: 18952781
references.citation: Plant J. 1997 Mar;11(3):605-12
references.pmid: 9107046
references.citation: Plant J. 1993 Sep;4(3):423-32
references.pmid: 7693132
references.citation: J Biol Chem. 2001 Apr 6;276(14):10730-6
references.pmid: 11154693
references.citation: Plant Mol Biol. 2006 Mar;60(5):663-77
references.pmid: 16649105
references.citation: Plant J. 2001 Sep;27(6):591-9
references.pmid: 11576442
references.citation: J Exp Bot. 2010 Jun;61(6):1853-67
references.pmid: 20348352
references.citation: Planta. 1996 Apr;198(4):532-541
references.pmid: 28321663
references.citation: Science. 1998 Nov 6;282(5391):1114-7
references.pmid: 9804548
references.citation: Proc Natl Acad Sci U S A. 1992 Jan 15;89(2):475-9
references.pmid: 11607251
references.citation: BMC Biol. 2005 Apr 14;3:11
references.pmid: 15831100
references.citation: Development. 2008 Oct;135(20):3345-54
references.pmid: 18787070
references.citation: Plant Physiol. 2007 Apr;143(4):1695-704
references.pmid: 17337532
references.citation: Plant Physiol. 1998 Jun;117(2):501-13
references.pmid: 9625703
references.citation: Plant Cell. 2004 Feb;16(2):319-31
references.pmid: 14742872
references.citation: Planta. 2008 Nov;228(6):929-40
references.pmid: 18654796
references.citation: Symp Soc Exp Biol. 1990;44:119-46
references.pmid: 2130510
references.citation: Nature. 2001 Sep 27;413(6854):425-8
references.pmid: 11574889
references.citation: J Biol Chem. 1999 Oct 1;274(40):28314-20
references.pmid: 10497189
references.citation: Plant J. 2011 Jan;65(2):282-94
references.pmid: 21223392
references.citation: Plant Physiol. 2003 Jan;131(1):254-63
references.pmid: 12529533
references.citation: Plant Physiol. 2009 Sep;151(1):155-67
references.pmid: 19633235
references.citation: PLoS One. 2009 Sep 24;4(9):e6648
references.pmid: 19777056
references.citation: Planta. 2002 Dec;216(2):302-8
references.pmid: 12447544
references.citation: Science. 2006 May 12;312(5775):914-8
references.pmid: 16601150
references.citation: Plant J. 2007 Apr;50(2):197-206
references.pmid: 17376160
references.citation: Genes Dev. 2001 Apr 1;15(7):902-11
references.pmid: 11297513
references.citation: Cell. 2010 Oct 1;143(1):99-110
references.pmid: 20887895
references.citation: J Plant Physiol. 2012 Jan 15;169(2):127-36
references.pmid: 22070974