title: Densitometric HPTLC analysis of 8-gingerol in Zingiber officinale extract and ginger-containing dietary supplements, teas and commercial creams.
abstract: OBJECTIVE
  To develop and validate a simple, accurate HPTLC method for the analysis of 8-gingerol and to determine the quantity of 8-gingerol in Zingiber officinale extract and ginger-containing dietary supplements, teas and commercial creams.
  
  
  METHODS
  The analysis was performed on 10×20 cm aluminium-backed plates coated with 0.2 mm layers of silica gel 60 F254 (E-Merck, Germany) with n-hexane: ethyl acetate 60: 40 (v/v) as mobile phase. Camag TLC Scanner III was used for the UV densitometric scanning at 569.
  
  
  RESULTS
  This system was found to give a compact spot of 8-gingerol at retention factor (Rf) value of (0.39±0.04) and linearity was found in the ranges 50-500 ng/spot (r (2)=0.9987). Limit of detection (12.76 ng/spot), limit of quantification (26.32 ng/spot), accuracy (less than 2 %) and recovery (ranging from 98.22-99.20) were found satisfactory.
  
  
  CONCLUSIONS
  The HPTLC method developed for quantification of 8-gingerol was found to be simple, accurate, reproducible, sensitive and is applicable to the analysis of 8-gingerol in Zingiber officinale extract and ginger-containing dietary supplements, teas and commercial creams.
authors.affiliation: Department of Pharmacognosy, College of Pharmacy, Salman Bin AbdulAziz University, P.O. Box 173, Al-Kharj 11942, Kingdom of Saudi Arabia. prawez_pharma@yahoo.com
authors.forename: Prawez
authors.initials: P
authors.lastname: Alam
mesh_terms: D002396:Catechols / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000191:economics / Q000379:methods; D002855:Chromatography, Thin Layer / Q000191:economics / Q000379:methods*; D003720:Densitometry / Q000191:economics / Q000379:methods; D019587:Dietary Supplements / Q000032:analysis*; D005233:Fatty Alcohols / Q000032:analysis*; D020939:Zingiber officinale / Q000737:chemistry*; D010936:Plant Extracts / Q000032:analysis*; D012680:Sensitivity and Specificity; D063465:Skin Cream / Q000032:analysis*; D013662:Tea / Q000737:chemistry*
chemical_list: D002396:Catechols; D005233:Fatty Alcohols; D010936:Plant Extracts; D013662:Tea; C534464:8-gingerol
keywords: 8-gingerol; HPTLC; ICH guidelines; Zingiber officinale
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