title: Efficacy and safety of hyaluronate membrane in the rabbit cecum-abdominal wall adhesion model.
abstract: OBJECTIVE
  Tissue adhesion is a well-known postsurgical phenomenon, causing pain, functional obstruction, and difficult reoperative surgery. To overcome these problems, various synthetic and natural polymer membranes have been developed as postoperative tissue adhesion barriers. However, limitation in their use has hindered its actual application. We prepared a hyaluronate membrane (HM) to evaluate its efficacy and safety as an adhesion barrier compared to a commercialized product (Interceed, Ethicon).
  
  
  METHODS
  To evaluate the antiadhesion effect, a cecum-abdominal wall abrasion model was adopted in a rabbit. The denuded cecum was covered by Interceed or HM or neither and apposed to the abdominal wall (each, n = 10). Four weeks after surgery, the level of adhesion was graded. Acute and chronic toxicity of the three groups were also evaluated.
  
  
  RESULTS
  Blood samples drawn to evaluate acute toxicity at postoperative day 3 and 7 showed no significant difference among the three groups. The grade and area of adhesion were significantly lower in the HM compared to those of the control and Interceed at four weeks after surgery. Histologic evaluations, which was carried out to estimate tissue reactions at the site of application, as well as to assess chronic toxicity for the major organs, were not significantly different in the three groups.
  
  
  CONCLUSIONS
  This study showed that the antiadhesion efficacy of HM was superior to commercialized antiadhesion membrane, Interceed. Low inflammatory response and nontoxicity were also demonstrated. From these results, we suggest that the HM is a good candidate as a tissue adhesion barrier.
authors.affiliation: Department of Surgery, CHA Bundang Medical Center, CHA University, Seongnam, Korea.
authors.forename: Jae Young
authors.initials: JY
authors.lastname: Kim
authors.forename: Wan Jin
authors.initials: WJ
authors.lastname: Cho
authors.forename: Jun Ho
authors.initials: JH
authors.lastname: Kim
authors.forename: Sae Hwan
authors.initials: SH
authors.lastname: Lim
authors.forename: Hyun Jung
authors.initials: HJ
authors.lastname: Kim
authors.forename: Young Woo
authors.initials: YW
authors.lastname: Lee
authors.forename: Sung Won
authors.initials: SW
authors.lastname: Kwon
keywords: Hyaluronic acid; Membranes; Tissue adhesions
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