title: Histological changes and antidiabetic activities of Icacina trichantha tuber extract in beta-cells of alloxan induced diabetic rats.
abstract: OBJECTIVE
  To investigate the antidiabetic, hypolipidaemic activities and histopathological changes of Icacina trichantha (I. trichantha) tuber extract in alloxan induced diabetic rats.
  
  
  METHODS
  In the present study, 80% methanol extract of I. trichantha tuber was tested on alloxan induced diabetic rats. They were randomly grouped into control (distilled water and glibenclamide) and experimental (200, 400 and 600 mg/kg body weight). Diabetes was induced by a single intraperitoneal injection of 160 mg/kg body weight of alloxan. Blood glucose levels were measured using blood glucose test strips with AccuCheck Advantage II glucometer at 1, 3, 6, and 24 h on the first day and 1 h after treatment on Day 7, 14 and 21. Blood samples were collected and centrifuged to separate serum for estimation of lipid profile and other biochemical parameters. Histopathological changes in diabetic rats pancreas were also studied after extract treatment.
  
  
  RESULTS
  Daily oral administration of I. trichantha tuber extract (200, 400, and 600 mg/kg body weight) and glibenclamide (2 mg/kg) showed beneficial effects on blood glucose level (P<0.01) as well as improving liver, kidney functions and hyperlipidaemia due to diabetes. The extract had a favourable effect on the histopathological changes of the pancreas in alloxan induced diabetes.
  
  
  CONCLUSIONS
  I. trichantha tuber extracts posses antidiabetic activities as well as improve liver and renal profile and total lipids levels. I. trichantha tuber extracts also have favourable effects to inhibit the histopathological changes of the pancreas in alloxan induced diabetes.
authors.affiliation: Department of Veterinary Physiology and Biochemistry, Faculty of Veterinary Medicine, University of Abuja, PMB 117, Abuja, Nigeria. onakpamm@yahoo.com
authors.forename: Onakpa Michael
authors.initials: OM
authors.lastname: Monday
authors.forename: Asuzu Isaac
authors.initials: AI
authors.lastname: Uzoma
mesh_terms: D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000139:chemically induced / Q000188:drug therapy*; D007004:Hypoglycemic Agents / Q000494:pharmacology; D000960:Hypolipidemic Agents / Q000494:pharmacology; D007340:Insulinoma / Q000188:drug therapy*; D019684:Magnoliopsida / Q000737:chemistry*; D008297:Male; D010190:Pancreatic Neoplasms / Q000188:drug therapy*; D010936:Plant Extracts / Q000494:pharmacology*; D035281:Plant Tubers / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D007004:Hypoglycemic Agents; D000960:Hypolipidemic Agents; D010936:Plant Extracts
keywords: Alloxan monohydrate; Diabetes mellitus; Glibenclamide; Icacina trichantha; Rats
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