title: Aging effects of plasma polymerized ethylenediamine (PPEDA) thin films on cell-adhesive implant coatings.
abstract: Thin plasma polymer films from ethylenediamine were deposited on planar substrates placed on the powered electrode of a low pressure capacitively coupled 13.56 MHz discharge. The chemical composition of the plasma polymer films was analyzed by Fourier Transform Infrared Reflection Absorption Spectroscopy (FT-IRRAS) as well as by X-ray photoelectron spectroscopy (XPS) after derivatization of the primary amino groups. The PPEDA films undergo an alteration during the storage in ambient air, particularly, due to reactions with oxygen. The molecular changes in PPEDA films were studied over a long-time period of 360 days. Simultaneously, the adhesion of human osteoblast-like cells MG-63 (ATCC) was investigated on PPEDA coated corundum blasted titanium alloy (Ti-6Al-4V), which is applied as implant material in orthopedic surgery. The cell adhesion was determined by flow cytometry and the cell shape was analyzed by scanning electron microscopy. Compared to uncoated reference samples a significantly enhanced cell adhesion and proliferation were measured for PPEDA coated samples, which have been maintained after long-time storage in ambient air and additional sterilization by γ-irradiation.
authors.affiliation: University of Greifswald, Institute of Physics, Felix-Hausdorff Str. 6, 17489 Greifswald, Germany. holger.testrich@uni-greifswald.de
authors.forename: H
authors.initials: H
authors.lastname: Testrich
authors.forename: H
authors.initials: H
authors.lastname: Rebl
authors.forename: B
authors.initials: B
authors.lastname: Finke
authors.forename: F
authors.initials: F
authors.lastname: Hempel
authors.forename: B
authors.initials: B
authors.lastname: Nebe
authors.forename: J
authors.initials: J
authors.lastname: Meichsner
mesh_terms: D000042:Absorption; D000497:Alloys; D002448:Cell Adhesion / Q000187:drug effects; D002460:Cell Line; D048430:Cell Shape / Q000187:drug effects; D020099:Coated Materials, Biocompatible / Q000494:pharmacology*; D004602:Elements; D005029:Ethylenediamines / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects / Q000648:ultrastructure; D056951:Photoelectron Spectroscopy; D058626:Plasma Gases / Q000494:pharmacology*; D058105:Polymerization / Q000187:drug effects*; D011312:Pressure; D019736:Prostheses and Implants; D017550:Spectroscopy, Fourier Transform Infrared; D013997:Time Factors; D014025:Titanium / Q000494:pharmacology
chemical_list: D000497:Alloys; D020099:Coated Materials, Biocompatible; D004602:Elements; D005029:Ethylenediamines; D058626:Plasma Gases; C031462:titanium alloy (TiAl6V4); C031234:ethylenediamine; D014025:Titanium
keywords: Aging effects; Ethylenediamine; Implant coating; Osteoblast adhesion; Plasma polymerization; RF plasma