title: Development and characterization of coaxially electrospun gelatin coated poly (3-hydroxybutyric acid) thin films as potential scaffolds for skin regeneration.
abstract: The morphology of fibers synthesized through electrospinning has been found to mimic extracellular matrix. Coaxially electrospun fibers of gelatin (sheath) coated poly (3-hydroxybutyric acid) (PHB) (core) was developed using 2,2,2 trifluoroethanol(TFE) and 1,1,1,3,3,3 hexafluoro-2-propanol(HFIP) as solvents respectively. The coaxial structure and coating of gelatin with PHB fibers was confirmed through transmission electron microscopy (TEM), scanning electron microscopy (SEM) and X-ray photoelectron spectroscopy (XPS). Thermal stability of the coaxially electrospun fibers was analyzed using thermogravimetric analysis(TGA), differential scanning calorimetry(DSC) and differential thermogravimetric analysis(DTA). Complete evaporation of solvent and gelatin grafting over PHB fibers was confirmed through attenuated total reflection-Fourier transformed infrared spectroscopy (ATR-FTIR). The coaxially electrospun fibers exhibited competent tensile properties for skin regeneration with high surface area and porosity. In vitro degradation studies proved the stability of fibers and its potential applications in tissue engineering. The fibers supported the growth of human dermal fibroblasts and keratinocytes with normal morphology indicating its potential as a scaffold for skin regeneration.
authors.affiliation: Bioproducts Lab, Central Leather Research Institute, Adyar, Chennai, India.
authors.forename: Naveen
authors.initials: N
authors.lastname: Nagiah
authors.forename: Lakshmi
authors.initials: L
authors.lastname: Madhavi
authors.forename: R
authors.initials: R
authors.lastname: Anitha
authors.forename: C
authors.initials: C
authors.lastname: Anandan
authors.forename: Natarajan Tirupattur
authors.initials: NT
authors.lastname: Srinivasan
authors.forename: Uma Tirichurapalli
authors.initials: UT
authors.lastname: Sivagnanam
mesh_terms: D002152:Calorimetry, Differential Scanning; D020099:Coated Materials, Biocompatible / Q000494:pharmacology*; D003432:Cross-Linking Reagents / Q000737:chemistry; D020405:Dermis / Q000166:cytology; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000648:ultrastructure; D005780:Gelatin / Q000494:pharmacology*; D006801:Humans; D006863:Hydrogen-Ion Concentration / Q000187:drug effects; D006885:Hydroxybutyrates / Q000494:pharmacology*; D046529:Microscopy, Electron, Transmission; D056951:Photoelectron Spectroscopy; D011091:Polyesters / Q000494:pharmacology*; D016062:Porosity; D000091402:Prohibitins; D012038:Regeneration / Q000187:drug effects*; D012867:Skin / Q000187:drug effects*; D017550:Spectroscopy, Fourier Transform Infrared; D013499:Surface Properties; D013718:Tensile Strength / Q000187:drug effects; D013818:Thermogravimetry; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*; D014867:Water / Q000737:chemistry; D000094122:Polyhydroxybutyrates
chemical_list: D020099:Coated Materials, Biocompatible; D003432:Cross-Linking Reagents; D005780:Gelatin; D006885:Hydroxybutyrates; D011091:Polyesters; D000091402:Prohibitins; D014867:Water; C000717328:PHB protein, human; C003182:poly-beta-hydroxybutyrate; D000094122:Polyhydroxybutyrates
keywords: Coaxial electrospinning; Gelatin; Poly (3-hydroxybutyric acid); Polymer matrix composites; Skin regeneration