title: Perfluorocarbon emulsion improves oxygen transport of normal and sickle cell human blood in vitro.
abstract: Perfluorocarbons (PFC) are compounds with high gas solubility that could help deliver O2 to tissues and have been suggested as adjunct therapy to ischemia. Using a newly designed in vitro system, we tested the hypothesis that a third generation PFC emulsion (Oxycyte) increased O2 transport of blood by measuring changes in O2 extraction ratio. The system included a computer-controlled pump and blood-gas exchange chambers to oxygenate and deoxygenate the blood from nine sickle cell disease (SCD) patients and five healthy donors. The flowing blood reached various levels of hemoglobin O2 saturation and O2 partial pressures (PO2), measured using a CO-oximeter and a blood gas analyzer. The mixtures were kept at physiological blood pressure and temperature, constant flow, normobaric conditions, and FiO2 = 0.30. After adding PFC, the measurements suggested an increase in the transport of O2 and CO. Addition of PFC resulted in larger PO2 difference from 15 ± 2 mmHg to 23 ± 2 mmHg. Using normal blood and blood from SCD patients, the average O2 extraction ratio (O2ER) after PFC was significantly higher than baseline. Addition of saline did not cause statistically significant changes. The data suggest increased (facilitated) O2 transport by this PFC emulsion in both normal and SCD blood.
authors.affiliation: Department of Physiology and Biophysics, Virginia Commonwealth University Reanimation Engineering Shock Center (VCURES), Virginia Commonwealth University, Richmond, Virginia, 23298-0695; Department of Emergency Medicine, Virginia Commonwealth University Reanimation Engineering Shock Center (VCURES), Virginia Commonwealth University, Richmond, Virginia, 23298-0695; US Army Institute of Surgical Research, Damage Control Resuscitation, San Antonio, Texas, 78234.
authors.forename: Ivo P
authors.initials: IP
authors.lastname: Torres Filho
authors.forename: José Ricardo P
authors.initials: JR
authors.lastname: Pedro
authors.forename: Srinivasan V
authors.initials: SV
authors.lastname: Narayanan
authors.forename: Nguyen M
authors.initials: NM
authors.lastname: Nguyen
authors.forename: Susan D
authors.initials: SD
authors.lastname: Roseff
authors.forename: Bruce D
authors.initials: BD
authors.lastname: Spiess
mesh_terms: D000755:Anemia, Sickle Cell / Q000097:blood*; D016022:Case-Control Studies; D004655:Emulsions; D005466:Fluorocarbons; D006801:Humans; D010100:Oxygen / Q000097:blood*
chemical_list: D004655:Emulsions; D005466:Fluorocarbons; D010100:Oxygen
keywords: cardiopulmonary bypass; facilitated diffusion; oxygen extraction ratio; oxygen transport; perfluorocarbon emulsion; sickle cell disease