title: Vitamin E modulates apoptosis and c-jun N-terminal kinase activation in ovarian torsion-detorsion injury.
abstract: The aim of this study was to evaluate the role of vitamin E in follicular degeneration and to assess histopathological and biochemical changes following ischemia-reperfusion (IR) injury in rat ovaries. Twenty-eight Wistar albino rats were randomly divided into four groups: sham, 4h torsion, 24h detorsion, and a vitamin E group. Thirty minutes before detorsion, a single dose of 200mg/kg vitamin E was administered intraperitoneally. The ovarian histology score was determined, serum levels of malondialdehyde (MDA) and myeloperoxidase (MPO) were measured. The apoptosis of granulosa cells and the phospho-c-jun N-terminal kinase (p-JNK) and phospho-p38 (p-p38) immunoreactivities of these cells were determined. MDA and MPO levels were significantly increased in the torsion and detorsion groups. Hemorrhage, edema, and congestion were also apparent in these groups. In addition, the apoptotic index and the immunoreactivity of p-JNK were highest in the detorsion group, which also showed marked follicular degeneration. However, p-p38 activity was not affected by torsion-detorsion (TD) induction. Vitamin E ameliorated TD-induced histological alterations. It also decreased serum levels of MDA and MPO, reduced the activity of p-JNK in the ovaries, and reduced numbers of apoptotic follicular cells. In conclusion, these data indicate that vitamin E attenuated ovarian follicular degeneration by inhibiting the immunoreactivity of p-JNK and reducing the apoptosis of granulosa cells.
authors.affiliation: Department of Histology and Embryology, Faculty of Medicine, Trakya University, Edirne, Turkey. Electronic address: melikesapmaz@yahoo.com.
authors.forename: Melike
authors.initials: M
authors.lastname: Sapmaz-Metin
authors.forename: Yeter
authors.initials: Y
authors.lastname: Topcu-Tarladacalisir
authors.forename: Yesim Hulya
authors.initials: YH
authors.lastname: Uz
authors.forename: Mustafa
authors.initials: M
authors.lastname: Inan
authors.forename: Imran Kurt
authors.initials: IK
authors.lastname: Omurlu
authors.forename: Aysegul
authors.initials: A
authors.lastname: Cerkezkayabekir
authors.forename: Gulnur
authors.initials: G
authors.lastname: Kizilay
authors.forename: Meryem
authors.initials: M
authors.lastname: Akpolat
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D004195:Disease Models, Animal; D004789:Enzyme Activation / Q000187:drug effects; D005260:Female; D007150:Immunohistochemistry; D020287:In Situ Nick-End Labeling; D048031:JNK Mitogen-Activated Protein Kinases / Q000378:metabolism*; D015227:Lipid Peroxidation / Q000187:drug effects; D010049:Ovarian Diseases / Q000378:metabolism* / Q000473:pathology; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000378:metabolism; D014102:Torsion Abnormality / Q000378:metabolism* / Q000473:pathology; D014810:Vitamin E / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D014810:Vitamin E; D048031:JNK Mitogen-Activated Protein Kinases
keywords: Apoptosis; JNK MAPK; Ovary; Rat; Torsion–detorsion; Vitamin E