title: Immunogenetic responses in calves to intranasal delivery of bovine respiratory syncytial virus (BRSV) epitopes encapsulated in poly (DL-lactide-co-glycolide) microparticles.
abstract: Bovine respiratory syncytial virus (BRSV) is the principal aetiological agent of the bovine respiratory disease complex. A BRSV subunit vaccine candidate consisting of two synthetic peptides representing putative protective epitopes on BRSV surface glycoproteins in soluble form or encapsulated in poly(lactide-co-glycolide) (PLG) microparticles were prepared. Calves (10 weeks old) with diminishing levels of BRSV-specific maternal antibody were intranasally administered a single dose of the different peptide formulations. Peptide-specific local immune responses (nasal secretion IgA), but not systemic humoral (serum IgG) or cellular responses (serum IFN-γ), were generated by all forms of peptide. There was a significant reduction in occurrence of respiratory disease in the animals inoculated with all peptide formulations compared to animals given PBS alone. Furthermore no adverse effects were observed in any of the animals post vaccination. These results suggest that intranasal immunisation with the peptide subunit vaccine does induce an as yet unidentified protective immune response.
authors.affiliation: Department of Veterinary Science, Queen's University Belfast, Stranmillis Road, Belfast BT9 5AG, Northern Ireland, UK. O.kavanagh@qub.ac.uk
authors.forename: Owen V
authors.initials: OV
authors.lastname: Kavanagh
authors.forename: Brian M
authors.initials: BM
authors.lastname: Adair
authors.forename: Michael
authors.initials: M
authors.lastname: Welsh
authors.forename: Bernadette
authors.initials: B
authors.lastname: Earley
mesh_terms: D000281:Administration, Intranasal; D000375:Aging; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D002417:Cattle; D000939:Epitopes / Q000737:chemistry / Q000276:immunology*; D005260:Female; D007112:Immunity, Maternally-Acquired; D019344:Lactic Acid / Q000737:chemistry*; D010316:Particle Size; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D018357:Respiratory Syncytial Virus Infections / Q000517:prevention & control / Q000662:veterinary*; D018112:Respiratory Syncytial Virus, Bovine / Q000276:immunology*; D014765:Viral Vaccines / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D000939:Epitopes; D014765:Viral Vaccines; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid
keywords: BRSV; Bovine respiratory syncytial virus; F-fusion glycoprotein; G-attachment glycoprotein; Intranasal inoculation; PLG; Poly(lactide-co-glycolide); Subunit vaccine