title: Overexpression of WDR62 is associated with centrosome amplification in human ovarian cancer.
abstract: OBJECTIVE
  To assess the clinical significance of WD40 repeat containing 62 (WDR62), a novel centrosome abnormalities-associated gene, in ovarian cancer.
  
  
  METHODS
  In this study, WDR62 expression was assessed by western blot (6 ovarian cancer cell lines) and immunohistochemistry (primary epithelial ovarian cancer clinical specimens), and clinical variables were collected by retrospective chart review. Centrosome amplification was assessed by immunofluorescence staining in ovarian cancer cell lines, and by immunohistochemistry staining in ovarian cancer samples.
  
  
  RESULTS
  Six ovarian cancer cell lines exhibited significant WDR62 protein overexpression, and amplification of centrosome. High-grade ovarian cancer specimens exhibited significantly stronger nuclear staining of WDR62 than low-grade ovarian carcinoma specimens (80.4% vs 41.3%; P<0.012). High WDR62 expression was strongly associated with supernumerary centrosome count in tumor cells (P < 0.001).
  
  
  CONCLUSIONS
  Our findings suggest that WDR62 overexpression is related to centrosome amplification in ovarian cancer. It may be a novel useful differentiation biomarker and a potential therapy target for OC. Further assessment of WDR62 expression is highly warranted in large, prospective studies.
authors.affiliation: Institute of Medical Sciences, Major Human Disease Tissue Bank, Xiangya Hospital, Central South University, Changsha, Hunan, China. yimingtao@hotmail.com.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhang
authors.forename: Yan
authors.initials: Y
authors.lastname: Tian
authors.forename: Jing-Jing
authors.initials: JJ
authors.lastname: Yu
authors.forename: Jie
authors.initials: J
authors.lastname: He
authors.forename: Jia
authors.initials: J
authors.lastname: Luo
authors.forename: Sai
authors.initials: S
authors.lastname: Zhang
authors.forename: Cen-E
authors.initials: CE
authors.lastname: Tang
authors.forename: Yi-Ming
authors.initials: YM
authors.lastname: Tao
references.citation: Am J Surg Pathol. 2005 Feb;29(2):218-24
references.pmid: 15644779
references.citation: Cancer Biol Ther. 2004 Dec;3(12):1250-3
references.pmid: 15477760
references.citation: Cancer Genet Cytogenet. 2002 Nov;139(1):78-83
references.pmid: 12547166
references.citation: Int J Biol Sci. 2011;7(8):1122-44
references.pmid: 22043171
references.citation: Int J Gynecol Pathol. 2004 Jan;23(1):41-4
references.pmid: 14668549
references.citation: Mol Biol Cell. 2010 Jan 1;21(1):117-30
references.pmid: 19910486
references.citation: Oncogene. 2008 Mar 13;27(12):1759-66
references.pmid: 17891171
references.citation: J Natl Cancer Inst. 2006 Nov 1;98(21):1558-70
references.pmid: 17077358
references.citation: Int J Gynaecol Obstet. 2008 May;101(2):205-10
references.pmid: 18199437
references.citation: Int J Cancer. 2006 Dec 15;119(12):2717-23
references.pmid: 17016823
references.citation: Mol Carcinog. 2003 Feb;36(2):53-9
references.pmid: 12557260
references.citation: J Cell Biochem. 2006 Dec 1;99(5):1240-50
references.pmid: 16817224
references.citation: Nat Genet. 2010 Nov;42(11):1015-20
references.pmid: 20890278
references.citation: Am J Surg Pathol. 2004 Apr;28(4):496-504
references.pmid: 15087669
references.citation: Int J Cancer. 2006 Nov 15;119(10):2304-12
references.pmid: 16894566
references.citation: Nature. 2010 Sep 9;467(7312):207-10
references.pmid: 20729831
references.citation: Nat Rev Cancer. 2007 Dec;7(12):911-24
references.pmid: 18004399
references.citation: Clin Cancer Res. 2007 Jul 15;13(14):4098-104
references.pmid: 17634535
references.citation: Cancer. 2000 Jun 1;88(11):2584-9
references.pmid: 10861437
references.citation: Nat Genet. 2010 Nov;42(11):1010-4
references.pmid: 20890279
references.citation: Nat Genet. 1998 Oct;20(2):189-93
references.pmid: 9771714
references.citation: Lancet. 2001 Jan 20;357(9251):176-82
references.pmid: 11213094
references.citation: Cancer Sci. 2006 Apr;97(4):271-6
references.pmid: 16630118
references.citation: Gynecol Oncol. 2011 Jan;120(1):11-7
references.pmid: 20937525