title: Rotavirus NSP1 inhibits interferon induced non-canonical NFκB activation by interacting with TNF receptor associated factor 2.
abstract: TNF receptor associated factor 2 (TRAF2) plays a very important role in cellular innate immune as well as inflammatory responses. Previous studies have reported TRAF2 mediated regulation of TNF and Interferon (IFN) induced canonical and non-canonical activation of NFκB. In this study, we show that rotavirus NSP1 targets TRAF2 to regulate IFN induced non-canonical NFκB activation. Here we found that rotavirus Non-Structural Protein-1 (NSP1) interacts with TRAF2 and degrades it in a proteasome dependent manner. C-terminal part of NSP1 was sufficient for interacting with TRAF2 but it alone could not degrade TRAF2. This inhibition of interferon mediated non-canonical NFκB activation by NSP1 may modulate inflammatory cytokine production after rotavirus infection to help the virus propagation.
authors.affiliation: Division of Virology, National Institute of Cholera and Enteric Diseases, P-33, C.I.T. Road Scheme-XM, Beliaghata, Kolkata 700010, West Bengal, India.
authors.forename: Parikshit
authors.initials: P
authors.lastname: Bagchi
authors.forename: Rahul
authors.initials: R
authors.lastname: Bhowmick
authors.forename: Satabdi
authors.initials: S
authors.lastname: Nandi
authors.forename: Mukti
authors.initials: M
authors.lastname: Kant Nayak
authors.forename: Mamta
authors.initials: M
authors.lastname: Chawla-Sarkar
mesh_terms: D054884:Host-Pathogen Interactions; D006801:Humans; D007372:Interferons / Q000276:immunology*; D016328:NF-kappa B / Q000037:antagonists & inhibitors*; D025941:Protein Interaction Mapping; D059748:Proteolysis; D012401:Rotavirus / Q000276:immunology*; D047992:TNF Receptor-Associated Factor 2 / Q000037:antagonists & inhibitors* / Q000378:metabolism; D017361:Viral Nonstructural Proteins / Q000378:metabolism*
chemical_list: D016328:NF-kappa B; D047992:TNF Receptor-Associated Factor 2; D017361:Viral Nonstructural Proteins; C104282:nsp1 protein, Rotavirus; D007372:Interferons
keywords: NFκB; NSP1; Rotavirus; TRAF2