title: A novel angiomatoid epithelioid sarcoma cell line, Asra-EPS, forming tumors with large cysts containing hemorrhagic fluid in vivo.
abstract: BACKGROUND
  Whereas we can use several human epithelioid sarcoma (ES) cell lines for basic and preclinical research, an angiomatoid ES cell line has not been reported to date. We have treated a case of an angiomatoid ES developing in the right upper extremity of a 67-year-old man.
  
  
  METHODS
  An angiomatoid ES cell line, Asra-EPS was newly established and characterized for its morphology, growth rate and chromosomal analysis. Tumorigenicity of Asra-EPS cells was also analyzed in athymic nude mice.
  
  
  RESULTS
  Asra-EPS cells were round, polygonal or spindle-shaped with an abundant cytoplasm and have been maintained continuously in vitro for over 150 passages during more than 15 months. These cells secreted cancer antigen 125 (CA 125), interleukin-6 (IL-6) and vascular endothelial growth factor (VEGF) into the culture medium. Asra-EPS cells were tumorigenic when implanted in nude mice with tumors reaching a volume of 1000 mm3 at around 50 days. Histological features of tumors formed in mice were essentially the same as those of the original tumor, exhibiting a multinodular proliferation of eosinophilic epithelioid and spindle-shaped cells with prominent areas of hemorrhage and blood-filled cystic spaces strikingly corresponding to the potential of hemorrhagic cyst formation in the original tumor. They showed immunopositive staining for cytokeratins (AE1/AE3 and CAM5.2), epithelial membrane antigen (EMA), vimentin, CD31, CD34 and CA 125, but negative for integrase interactor 1 (INI-1) and factor VIII-related antigen.
  
  
  CONCLUSIONS
  The established cell line represents a biologically relevant new tool to investigate the molecular pathology of human angiomatoid ES and to evaluate the efficacy of novel therapeutics both in vitro and in vivo.
authors.affiliation: Department of Orthopaedic Surgery, Osaka University Graduate School of Medicine, 2-2 Yamadaoka, Suita, Osaka 565-0871, Japan.
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Imura
authors.forename: Norifumi
authors.initials: N
authors.lastname: Naka
authors.forename: Hidetatsu
authors.initials: H
authors.lastname: Outani
authors.forename: Hirohiko
authors.initials: H
authors.lastname: Yasui
authors.forename: Satoshi
authors.initials: S
authors.lastname: Takenaka
authors.forename: Ken-ichiro
authors.initials: K
authors.lastname: Hamada
authors.forename: Ritsuro
authors.initials: R
authors.lastname: Ozaki
authors.forename: Mitsunori
authors.initials: M
authors.lastname: Kaya
authors.forename: Ken-ichi
authors.initials: K
authors.lastname: Yoshida
authors.forename: Eiichi
authors.initials: E
authors.lastname: Morii
authors.forename: Akira
authors.initials: A
authors.lastname: Myoui
authors.forename: Hideki
authors.initials: H
authors.lastname: Yoshikawa
mesh_terms: D000368:Aged; D000818:Animals; D014408:Biomarkers, Tumor / Q000378:metabolism; D001706:Biopsy; D015153:Blotting, Western; D049109:Cell Proliferation; D048430:Cell Shape; D002877:Chromosomes, Human; D006391:Hemangioma / Q000098:blood supply / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D006470:Hemorrhage / Q000473:pathology*; D006801:Humans; D007150:Immunohistochemistry; D008279:Magnetic Resonance Imaging; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D018297:Neoplasms, Cystic, Mucinous, and Serous / Q000098:blood supply / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D012509:Sarcoma / Q000098:blood supply / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D013997:Time Factors; D047368:Tumor Burden; D014410:Tumor Stem Cell Assay
chemical_list: D014408:Biomarkers, Tumor
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