title: Pleiohomeotic interacts with the core transcription elongation factor Spt5 to regulate gene expression in Drosophila.
abstract: The early elongation checkpoint regulated by Positive Transcription Elongation Factor b (P-TEFb) is a critical control point for the expression of many genes. Spt5 interacts directly with RNA polymerase II and has an essential role in establishing this checkpoint, and also for further transcript elongation. Here we demonstrate that Drosophila Spt5 interacts both physically and genetically with the Polycomb Group (PcG) protein Pleiohomeotic (Pho), and the majority of Pho binding sites overlap with Spt5 binding sites across the genome in S2 cells. Our results indicate that Pho can interact with Spt5 to regulate transcription elongation in a gene specific manner.
authors.affiliation: Transcriptional Regulation Group, UCL Cancer Institute, University College London, London, United Kingdom.
authors.forename: Robert
authors.initials: R
authors.lastname: Harvey
authors.forename: Eugene
authors.initials: E
authors.lastname: Schuster
authors.forename: Barbara H
authors.initials: BH
authors.lastname: Jennings
mesh_terms: D000818:Animals; D001665:Binding Sites; D002868:Chromosomal Proteins, Non-Histone / Q000378:metabolism*; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*; D005260:Female; D005786:Gene Expression Regulation; D023281:Genomics; D008297:Male; D063146:Polycomb-Group Proteins / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D013489:Suppression, Genetic; D035602:Transcriptional Elongation Factors / Q000378:metabolism*; D014921:Wings, Animal / Q000254:growth & development
chemical_list: D002868:Chromosomal Proteins, Non-Histone; D029721:Drosophila Proteins; D063146:Polycomb-Group Proteins; D035602:Transcriptional Elongation Factors; C113295:pho protein, Drosophila; C069940:SPT5 transcriptional elongation factor
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