title: High-power femtosecond-terahertz pulse induces a wound response in mouse skin.
abstract: Terahertz (THz) technology has emerged for biomedical applications such as scanning, molecular spectroscopy, and medical imaging. Although a thorough assessment to predict potential concerns has to precede before practical utilization of THz source, the biological effect of THz radiation is not yet fully understood with scant related investigations. Here, we applied a femtosecond-terahertz (fs-THz) pulse to mouse skin to evaluate non-thermal effects of THz radiation. Analysis of the genome-wide expression profile in fs-THz-irradiated skin indicated that wound responses were predominantly mediated by transforming growth factor-beta (TGF-β) signaling pathways. We validated NFκB1- and Smad3/4-mediated transcriptional activation in fs-THz-irradiated skin by chromatin immunoprecipitation assay. Repeated fs-THz radiation delayed the closure of mouse skin punch wounds due to up-regulation of TGF-β. These findings suggest that fs-THz radiation initiate a wound-like signal in skin with increased expression of TGF-β and activation of its downstream target genes, which perturbs the wound healing process in vivo.
authors.affiliation: Departments of Biomedical Sciences, College of Medicine, Seoul National University, Seoul 110-799, Korea.
authors.forename: Kyu-Tae
authors.initials: KT
authors.lastname: Kim
authors.forename: Jaehun
authors.initials: J
authors.lastname: Park
authors.forename: Sung Jin
authors.initials: SJ
authors.lastname: Jo
authors.forename: Seonghoon
authors.initials: S
authors.lastname: Jung
authors.forename: Oh Sang
authors.initials: OS
authors.lastname: Kwon
authors.forename: Gian Piero
authors.initials: GP
authors.lastname: Gallerano
authors.forename: Woong-Yang
authors.initials: WY
authors.lastname: Park
authors.forename: Gun-Sik
authors.initials: GS
authors.lastname: Park
mesh_terms: D000818:Animals; D016207:Cytokines / Q000276:immunology; D004307:Dose-Response Relationship, Radiation; D051379:Mice; D008810:Mice, Inbred C57BL; D011829:Radiation Dosage; D011832:Radiation Injuries / Q000209:etiology* / Q000276:immunology*; D012867:Skin / Q000276:immunology* / Q000293:injuries*; D055053:Terahertz Radiation / Q000009:adverse effects*; D014945:Wound Healing / Q000276:immunology* / Q000528:radiation effects*
chemical_list: D016207:Cytokines
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