title: Expression of a GABA(B) - receptor in olfactory sensory neurons of sensilla trichodea on the male antenna of the moth Heliothis virescens.
abstract: In the olfactory pathway of Drosophila, a GABAB receptor mediated presynaptic gain control mechanism at the first synapse between olfactory sensory neurons (OSNs) and projection neurons has been suggested to play a critical role in setting the sensitivity and detection range of the sensory system. To approach the question if such a mechanism may be realized in the pheromone recognition system of male moths in this study attempts were made to explore if moth's pheromone-responsive cells express a GABAB- receptor. Employing a combination of genome analysis, RT-PCR experiments and screening of an antennal cDNA library we have identified a cDNA which encodes the GABAB-R1 receptor of Heliothis virescens. Moreover, based on the HvirGABAB-R1 sequence we could predict a GABAB-R1 protein from genome sequences of the silkmoth Bombyx mori. To assess whether HvirGABAB-R1 is expressed in OSNs of male antenna we performed whole-mount in situ hybridization (WM-ISH) experiments. Several HvirGABAB-R1 positive cells were visualized under long sensilla trichodea, known to contain pheromone-responsive OSNs. In parallel it was shown that cells under long trichoid hairs were labelled with pheromone receptor specific probes. In addition, the HvirGABAB-R1 specific probe also labelled several cells under shorter olfactory sensilla, but never stained cells under mechanosensory/gustatory sensilla chaetica. Together, the results indicate that a GABAB receptor is expressed in pheromone-responsive OSNs of H. virescens and suggest a presynaptic gain control mechanism in the axon terminals of these cells.
authors.affiliation: University of Hohenheim, Institute of Physiology, Stuttgart, Germany.
authors.forename: Pablo
authors.initials: P
authors.lastname: Pregitzer
authors.forename: Anna
authors.initials: A
authors.lastname: Schultze
authors.forename: Klaus
authors.initials: K
authors.lastname: Raming
authors.forename: Heinz
authors.initials: H
authors.lastname: Breer
authors.forename: Jürgen
authors.initials: J
authors.lastname: Krieger
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D008297:Male; D008969:Molecular Sequence Data; D009036:Moths; D018080:Receptors, GABA-B / Q000096:biosynthesis*; D043563:Receptors, Pheromone / Q000096:biosynthesis*; D058618:Sensilla / Q000378:metabolism; D011984:Sensory Receptor Cells / Q000378:metabolism; D016415:Sequence Alignment
chemical_list: D018080:Receptors, GABA-B; D043563:Receptors, Pheromone
keywords: GABA; in situ hybridization.; moth; olfaction; pheromone
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