title: Proteomic analysis in peritoneal dialysis patients with different peritoneal transport characteristics.
abstract: Peritoneal membranes can be categorized as high, high average, low average, and low transporters, based on the removal or transport rate of solutes. In this study, we used proteomic analysis to determine the differences in proteins removed by different types of peritoneal membranes. Peritoneal transport characteristics in patients who received peritoneal dialysis therapy were assessed by a peritoneal equilibration test. Two-dimensional differential gel electrophoresis technology followed by quantitative analysis was performed to study the variation in protein expression from peritoneal dialysis effluents (PDE) among different groups. Proteins were identified by MALDI-TOF-MS/MS analyses. Further validation in PDE or serum was performed utilizing ELISA analysis. Proteomics analysis revealed ten protein spots with significant differences in intensity levels among different groups, including vitamin D-binding protein, complement C3, apolipoprotein-A1, complement factor C4A, haptoglobin, alpha-1 antitrypsin, immunoglobulin kappa light chain, alpha-2-microglobulin, retinol-binding protein 4 and transthyretin. The levels of vitamin D-binding protein, complement C3, and apolipoprotein-A1 in PDE derived from different groups were greatly varied (P<0.05). However, no significant difference was found in the serum levels of these proteins among different groups (P>0.05 for all groups). This study provides a novel overview of the differences in PDE proteomes of four types of peritoneal membranes. Vitamin D-binding protein, complement C3, and apolipoprotein-A1 showed enhanced expression in PDE of patients with high transporter.
authors.affiliation: Department of Nephrology, The First Affiliated Hospital, Sun Yat-sen University, Key Laboratory of Nephrology, Ministry of Health, Guangzhou, Guangdong, China.
authors.forename: Qiong
authors.initials: Q
authors.lastname: Wen
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.forename: Hai-Ping
authors.initials: HP
authors.lastname: Mao
authors.forename: Xue-Qing
authors.initials: XQ
authors.lastname: Tang
authors.forename: Rong
authors.initials: R
authors.lastname: Rong
authors.forename: Jin-Jin
authors.initials: JJ
authors.lastname: Fan
authors.forename: Xue-Qing
authors.initials: XQ
authors.lastname: Yu
mesh_terms: D000328:Adult; D016632:Apolipoprotein A-I / Q000378:metabolism; D001692:Biological Transport / Q000502:physiology*; D003176:Complement C3 / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D005921:Glomerulonephritis / Q000503:physiopathology* / Q000628:therapy; D006801:Humans; D007676:Kidney Failure, Chronic / Q000503:physiopathology / Q000628:therapy; D008297:Male; D008875:Middle Aged; D010530:Peritoneal Dialysis; D010537:Peritoneum / Q000378:metabolism*; D040901:Proteomics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014809:Vitamin D-Binding Protein / Q000378:metabolism*
chemical_list: D016632:Apolipoprotein A-I; D003176:Complement C3; D014809:Vitamin D-Binding Protein
keywords: Peritoneal dialysis; Peritoneal transport; Proteomic analysis