title: Use of defatted flaxseed meal reduces precancerous colon lesions in C57BL/6 mice.
abstract: OBJECTIVE
  To investigate the hemopreventive effect of defatted flaxseed meal in C57BL/6 mice after induction of precancerous colon lesions with 1.2-dimethylhydrazine (DMH).
  
  
  METHODS
  Thirty-six 12-week-old C57BL/6 mice were divided into three treatment groups(n=12 in each group): (1) diet with 10% defatted flaxseed meal; (2) diet with defatted flaxseed meal and precancerous colon lesions induced by DMH; and (3) precancerous colon lesions induced by DMH, without defatted flaxseed meal. The incidence of aberrant crypt foci (ACF), oxidative processes, expression of tumor suppressor proteins and cyclins, as well as the profile of short-chain fatty acids (SCFA) in animal feces were investigated in the presence and absence of DMH.
  
  
  RESULTS
  The rats consuming defatted flaxseed meals showed lesions with lower multiplicity and a reduced incidence of lesions. No changes in the expression of tumor suppressor proteins and those involved in cell cycle control were detected.
  
  
  CONCLUSIONS
  Defatted flaxseed meal protected the distal colon of mice from precancerous lesions.
authors.affiliation: Department of Nutrition, Federal University of Juiz de Fora, Minas Gerais, Brazil.
authors.forename: Antônio Frederico de Freitas
authors.initials: AF
authors.lastname: Gomides
authors.forename: Sérgio Oliveira de
authors.initials: SO
authors.lastname: Paula
authors.forename: Damiana Diniz
authors.initials: DD
authors.lastname: Rosa
authors.forename: Leandro Licusi de
authors.initials: LL
authors.lastname: Oliveira
authors.forename: Débora Silva
authors.initials: DS
authors.lastname: Comastri
authors.forename: Maria do Carmo Gouveia
authors.initials: Mdo C
authors.lastname: Peluzio
mesh_terms: D019813:1,2-Dimethylhydrazine; D058739:Aberrant Crypt Foci; D000818:Animals; D002273:Carcinogens; D003106:Colon / Q000293:injuries*; D003110:Colonic Neoplasms / Q000139:chemically induced / Q000517:prevention & control*; D005227:Fatty Acids / Q000032:analysis; D019597:Flax / Q000737:chemistry*; D051379:Mice; D008810:Mice, Inbred C57BL; D018384:Oxidative Stress; D010936:Plant Extracts / Q000494:pharmacology*; D011230:Precancerous Conditions / Q000139:chemically induced / Q000517:prevention & control*; D060888:Real-Time Polymerase Chain Reaction; D015203:Reproducibility of Results; D012639:Seeds / Q000737:chemistry*; D013997:Time Factors
chemical_list: D002273:Carcinogens; D005227:Fatty Acids; D010936:Plant Extracts; D019813:1,2-Dimethylhydrazine