title: Human mesenchymal stem cells creating an immunosuppressive environment and promote breast cancer in mice.
abstract: Human mesenchymal stem cells (hMSC) can home to tumor sites and promote tumor growth. The effects of hMSC on tumor growth are controversial and involvement of hMSC in tumor immunology has not been adequately addressed. Therefore, we investigated whether injection of hMSC affects tumor appearance, growth and metastasis, and anti-tumor immunity in an experimental animal model of metastatic breast cancer. Injection of hMSC in BALB/c mice bearing mammary carcinoma promoted tumor growth and metastasis, which was accompanied by lower cytotoxic activity of splenocytes, NK cells and CD8⁺ T cells in vitro. Tumor-bearing mice that received hMSC had significantly lower percentages of CD3⁺NKp46⁺ NKT-like, higher percentages of CD4⁺Foxp3⁺ T cells, increased serum levels of Th2 and decreased serum levels of Th1 cytokines, and significantly higher number of CD4⁺ cells expressing IL-10. These results demonstrate that immunosuppressive environment created by hMSC promoted breast tumor growth and metastasis in mice.
authors.affiliation: Center for Molecular Medicine and Stem Cell Research, Faculty of Medical Sciences, University of Kragujevac, Kragujevac, Serbia.
authors.forename: Biljana
authors.initials: B
authors.lastname: Ljujic
authors.forename: Marija
authors.initials: M
authors.lastname: Milovanovic
authors.forename: Vladislav
authors.initials: V
authors.lastname: Volarevic
authors.forename: Bridgid
authors.initials: B
authors.lastname: Murray
authors.forename: Diana
authors.initials: D
authors.lastname: Bugarski
authors.forename: Stefan
authors.initials: S
authors.lastname: Przyborski
authors.forename: Nebojsa
authors.initials: N
authors.lastname: Arsenijevic
authors.forename: Miodrag L
authors.initials: ML
authors.lastname: Lukic
authors.forename: Miodrag
authors.initials: M
authors.lastname: Stojkovic
mesh_terms: D000818:Animals; D001943:Breast Neoplasms / Q000276:immunology* / Q000378:metabolism* / Q000473:pathology; D002465:Cell Movement / Q000276:immunology; D002471:Cell Transformation, Neoplastic / Q000276:immunology / Q000378:metabolism; D016207:Cytokines / Q000378:metabolism; D003602:Cytotoxicity, Immunologic; D005260:Female; D006801:Humans; D007108:Immune Tolerance; D007694:Killer Cells, Natural; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000378:metabolism*; D051379:Mice; D055611:Natural Killer T-Cells / Q000276:immunology; D009362:Neoplasm Metastasis; D013154:Spleen / Q000276:immunology; D016176:T-Lymphocyte Subsets / Q000276:immunology / Q000378:metabolism; D047368:Tumor Burden / Q000276:immunology; D059016:Tumor Microenvironment / Q000276:immunology*
chemical_list: D016207:Cytokines
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