title: Phytochemical screening and cytotoxicity studies of Chrysophyllum pruniforme Pierre ex Engl. barks.
abstract: BACKGROUND
  Chrysophyllum pruniforme of family sapotaceae is a plant used in traditional medicine in Gabon.
  
  
  METHODS
  In this study, C. pruniforme barks were subjected to phytochemical screening and cytotoxicity investigations. Different concentrations of aqueous and total phenolic extract were tested on mice and on human erythrocytes.
  
  
  RESULTS
  Phytochemical screening of C. pruniforme barks revealed the presence of flavonoids, saponins, and tannins, reducing sugars, polyphenols and traces of anthraquinones. When tested in vitro, aqueous and the phenolic extracts showed hemolytic activities on human erythrocytes with phenolic compounds being more cytotoxic than aqueous extracts. In vivo study of toxicity, allowed to determine the LD50 at 90 mg/kg for the doses of 50, 150 and 250 mg/kg of body weight.
  
  
  CONCLUSIONS
  These data indicate in one hand that C. pruniforme is rich in phenolic compounds and that the aqueous and total phenolic extracts could be considered as toxic for mice and maybe potentially toxic to humans in the other hand.
authors.affiliation: Pharmacopea and Traditional Medicine Institute, National Center of Technological and Scientific Research, Lambaréné, Gabon.
authors.forename: Sophie Aboughe
authors.initials: SA
authors.lastname: Angone
authors.forename: L
authors.initials: L
authors.lastname: Mewono
authors.forename: M Boukandou
authors.initials: MB
authors.lastname: Mounanga
authors.forename: S
authors.initials: S
authors.lastname: Medzegue
authors.forename: H F
authors.initials: HF
authors.lastname: Ella Mendene
authors.forename: J G
authors.initials: JG
authors.lastname: Mba Ndong
authors.forename: J F
authors.initials: JF
authors.lastname: Djoba Siawaya
authors.forename: A
authors.initials: A
authors.lastname: Souza
keywords: Chrysophyllum; cytotoxicity polyphenols; phytochemical screening; pruniforme
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