title: Antiaging, antistress and ROS scavenging activity of crude extract of Ocimum sanctum (L.) in Caenorhabditis elegans (Maupas, 1900).
abstract: Since aging is the most important risk factor for variety of diseases, the discovery of a wide range of chemical modulators of aging in model organisms encourages new strategies for targeting age associated diseases. Simple genetic manipulation leads to long-lived and healthy animals, so any compound which could have similar effect would prove a boon to mankind. In the present study, effect of different pharmacological doses (1.0, 0.1, 0.01 and 0.001 mg/mL) of O. sanctum crude extract were used to determine their impact on life span, thermotolerance and ROS scavenging activities in C. elegans. The results revealed that 1 mg/mL of O. sanctum extract significantly extended the life span of C. elegans. The extract also proved to be a strong free radical scavenger and increased resistance against thermal stress. It is also suggested that the protective and life span extending action of the crude extract is not only due to its antioxidant capacity but may also be mediated by modulation of some signaling pathways. Thus, in addition to all the known medicinal property of Ocimum, it is capable of increasing stress tolerance and life span in C. elegans.
authors.affiliation: Microbial Technology and Nematology Department, CSIR-Central Institute of Medicinal and Aromatic Plants (CIMAP), P.O. CIMAP, Lucknow 226015, India. r.pandey@cimap.res.in
authors.forename: Rakesh
authors.initials: R
authors.lastname: Pandey
authors.forename: Shipra
authors.initials: S
authors.lastname: Gupta
authors.forename: Virendra
authors.initials: V
authors.lastname: Shukla
authors.forename: Sudeep
authors.initials: S
authors.lastname: Tandon
authors.forename: Vibha
authors.initials: V
authors.lastname: Shukla
mesh_terms: D000375:Aging / Q000187:drug effects*; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D017173:Caenorhabditis elegans / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D029742:Caenorhabditis elegans Proteins / Q000235:genetics / Q000378:metabolism; D049109:Cell Proliferation; D002633:Chemotaxis / Q000187:drug effects; D045424:Complex Mixtures / Q000494:pharmacology*; D004777:Environment; D016166:Free Radical Scavengers / Q000494:pharmacology*; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism; D006358:Hot Temperature; D006861:Hydrogen Peroxide / Q000378:metabolism; D031339:Ocimum / Q000737:chemistry*; D018384:Oxidative Stress / Q000187:drug effects*; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction / Q000187:drug effects; D037761:Sirtuins / Q000235:genetics / Q000378:metabolism
chemical_list: D000975:Antioxidants; D029742:Caenorhabditis elegans Proteins; D045424:Complex Mixtures; D016166:Free Radical Scavengers; D018840:HSP70 Heat-Shock Proteins; D012333:RNA, Messenger; D006861:Hydrogen Peroxide; C507014:SIR-2.1 protein, C elegans; D037761:Sirtuins