title: Engineering multiple biological functional motifs into a blank collagen-like protein template from Streptococcus pyogenes.
abstract: Bacterially derived triple-helical, collagen-like proteins are attractive as potential biomedical materials. The collagen-like domain of the Scl2 protein from S. pyogenes lacks any specific binding sites for mammalian cells yet possesses the inherent structural integrity of the collagen triple-helix of animal collagens. It can, therefore, be considered as a structurally-stable "blank slate" into which various defined, biological sequences, derived from animal collagens, can be added by substitutions or insertions, to enable production of novel designed materials to fit specific functional requirements. In the present study, we have used site directed mutagenesis to substitute two functional sequences, one for heparin binding and the other for integrin binding, into different locations in the triple-helical structure. This provided three new constructs, two containing the single substitutions and one containing both substitutions. The stability of these constructs was marginally reduced when compared to the unmodified sequence. When compared to the unmodified bacterial collagen, both the modified collagens that contain the heparin binding site showed marked binding of fluorescently labeled heparin. Similarly, the modified collagens from both constructs containing the integrin binding site showed significant adhesion of L929 cells that are known to possess the appropriate integrin receptor. C2C12 cells that lack any appropriate integrins did not bind. These data show that bacterial collagen-like sequences can be modified to act like natural extracellular matrix collagens by inserting one or more unique biological domains with defined function.
authors.affiliation: CSIRO Materials Science and Engineering, Bayview Avenue, Clayton, 3169, Australia.
authors.forename: Yong Y
authors.initials: YY
authors.lastname: Peng
authors.forename: Violet
authors.initials: V
authors.lastname: Stoichevska
authors.forename: Kristin
authors.initials: K
authors.lastname: Schacht
authors.forename: Jerome A
authors.initials: JA
authors.lastname: Werkmeister
authors.forename: John A M
authors.initials: JA
authors.lastname: Ramshaw
mesh_terms: D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D003094:Collagen / Q000737:chemistry / Q000378:metabolism*; D017931:DNA Primers; D004591:Electrophoresis, Polyacrylamide Gel; D016023:Integrins / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011485:Protein Binding; D015202:Protein Engineering; D013297:Streptococcus pyogenes / Q000378:metabolism*
chemical_list: D017931:DNA Primers; D016023:Integrins; D003094:Collagen
keywords: collagen; heparin binding; integrin binding; triple helix