title: Methylation of a novel CpG island of intron 1 is associated with steroidogenic factor 1 expression in endometriotic stromal cells.
abstract: Steroidogenic factor 1 (SF-1), a transcriptional factor essential for estrogen biosynthesis, is undetectable in endometrial stromal cells and aberrantly expressed in endometriotic stromal cells.
  
  
  OBJECTIVE
  We tried to gain further insight into the mechanism for differential SF-1 expression in endometrial and endometriotic stromal cells.
  
  
  METHODS
  We had previously identified a novel CpG island in SF-1, which is located in the downstream intron 1 region. Here, we evaluated the methylation status of this CpG island.
  
  
  METHODS
  We obtained the eutopic endometrium from disease-free participants (n = 8) and the walls of cystic endometriosis lesions of the ovaries from another group of participants (n = 8). None of the patients had received any preoperative hormonal therapy.
  
  
  METHODS
  Stromal cells were isolated from these 2 types of tissues and subjected to DNA bisulfite treatment and sequence analysis.
  
  
  RESULTS
  The SF-1 messenger RNA (mRNA) levels in endometriotic stromal cells were significantly higher than those in endometrial stromal cells. Bisulfite sequencing showed strikingly increased methylation of a 1-kbp region around the previously identified CpG island in endometriotic cells compared with endometrial cells (P < .001). A strong correlation between SF-1 mRNA levels and percentage methylation of the intron 1 region of the SF-1 gene was observed in endometriotic cells (Spearman correlation coefficient, .96; P < .001).
  
  
  CONCLUSIONS
  Methylation of the intron 1 region of the SF-1 gene is associated with its expression in endometriotic cells. This CpG island therefore plays an important role in regulating SF-1 expression.
authors.affiliation: 1Department of Obstetrics and Gynecology, First Hospital of Beijing University, Beijing, P. R. China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Xue
authors.forename: Yang
authors.initials: Y
authors.lastname: Xu
authors.forename: Huixia
authors.initials: H
authors.lastname: Yang
authors.forename: Lei
authors.initials: L
authors.lastname: Zhang
authors.forename: Jing
authors.initials: J
authors.lastname: Shang
authors.forename: Cheng
authors.initials: C
authors.lastname: Zeng
authors.forename: Ping
authors.initials: P
authors.lastname: Yin
authors.forename: Serdar E
authors.initials: SE
authors.lastname: Bulun
mesh_terms: D000328:Adult; D002478:Cells, Cultured; D018899:CpG Islands / Q000502:physiology*; D004717:Endometrium / Q000378:metabolism* / Q000473:pathology; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D007438:Introns / Q000502:physiology*; D008745:Methylation; D054339:Steroidogenic Factor 1 / Q000096:biosynthesis*; D017154:Stromal Cells / Q000378:metabolism / Q000473:pathology
chemical_list: C515057:NR5A1 protein, human; D054339:Steroidogenic Factor 1
keywords: CpG island; DNA methylation; SF-1; endometriosis; intron
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