title: The anticonvulsant and sedative effects of Gladiolus dalenii extracts in mice.
abstract: Gladiolus dalenii Van Geel is a medicinal plant commonly used in traditional medicine in Africa to treat epilepsy and many other diseases. Two in vivo mouse models (maximal electroshock and pentylenetetrazol-induced convulsions) were used to evaluate the anticonvulsant activities of the plant extracts. Diazepam-induced sleep was used for the evaluation of the sedative properties. The macerated extract of G. dalenii protected 100 and 83.3% of mice against PTZ- and MES-induced seizures, respectively. The aqueous extract of G. dalenii protected 100 and 83.3% of mice against PTZ- and MES-induced seizures, respectively. The lyophilized extract of G. dalenii also protected 100 and 83.3% of mice against PTZ- and MES-induced seizures, respectively. The coadministration of G. dalenii with diazepam resulted in an additive effect, while the coadministration of G. dalenii with flumazenil or FG7142 resulted in antagonistic effects. The macerate of G. dalenii also exerted sedative activity by reducing the latency time to sleep and increasing the total duration of sleep induced by diazepam. The sleeping time increased from 16±3min in the control group to 118±11min at a dose of 150mg/kg of G. dalenii. The effects of G. dalenii suggested the presence of anticonvulsant and sedative activities that might show efficacy against secondarily generalized tonic-clonic seizures and primary generalized seizures and insomnia in humans.
authors.affiliation: Department of Biological Sciences, Faculty of Science, University of Ngaoundéré, P.O. Box 454, Ngaoundéré, Cameroon.
authors.forename: G T
authors.initials: GT
authors.lastname: Ngoupaye
authors.forename: E
authors.initials: E
authors.lastname: Ngo Bum
authors.forename: E
authors.initials: E
authors.lastname: Ngah
authors.forename: E
authors.initials: E
authors.lastname: Talla
authors.forename: F C O
authors.initials: FC
authors.lastname: Moto
authors.forename: G S
authors.initials: GS
authors.lastname: Taiwe
authors.forename: A
authors.initials: A
authors.lastname: Rakotonirina
authors.forename: S V
authors.initials: SV
authors.lastname: Rakotonirina
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D000927:Anticonvulsants / Q000627:therapeutic use*; D001921:Brain / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D003975:Diazepam / Q000627:therapeutic use; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D004359:Drug Therapy, Combination; D006575:Heterocyclic Compounds, 3-Ring; D006993:Hypnotics and Sedatives; D008297:Male; D051379:Mice; D010433:Pentylenetetrazole / Q000633:toxicity; D008517:Phytotherapy / Q000379:methods*; D010936:Plant Extracts / Q000494:pharmacology*; D011597:Psychomotor Performance / Q000187:drug effects; D012640:Seizures / Q000188:drug therapy* / Q000209:etiology / Q000473:pathology; D012890:Sleep / Q000187:drug effects*; D005680:gamma-Aminobutyric Acid / Q000378:metabolism
chemical_list: D000927:Anticonvulsants; D006575:Heterocyclic Compounds, 3-Ring; D006993:Hypnotics and Sedatives; D010936:Plant Extracts; C532317:daldinin A; D005680:gamma-Aminobutyric Acid; D003975:Diazepam; D010433:Pentylenetetrazole
keywords: Botanical medicine; Epilepsy; Gladiolus dalenii; Herbal medicine; Insomnia; Traditional medicine