title: Using optical profilometry to characterize cell membrane roughness influenced by amyloid-beta 42 aggregates and electric fields.
abstract: The membrane roughness of Neuro-2a neroblastoma cells is measured by using noninterferometric wide-field optical profilometry. The cells are treated with the fibril and oligomer conformers of amyloid-beta (Aβ) 42, which is a peptide of 42 amino acids related to the development of Alzheimer's disease. We find that both the Aβ42 fibrils and Aβ42 oligomers reduced the cell membrane roughness, but the effect of Aβ42 oligomers was faster and stronger than that of the fibrils. We also apply direct-current electric field (dcEF) stimulations on the cells. A dcEF of 300 mV/mm can increase the membrane roughness under the treatment of Aβ42. These results suggest that Aβ42 can decrease the membrane compliance of live neuroblastoma cells, and dcEFs may counteract this effect.
authors.affiliation: Research Center for Applied Sciences, Academia Sinica, Taipei 11529, Taiwan.
authors.forename: Huei-Jyuan
authors.initials: HJ
authors.lastname: Pan
authors.forename: Ruei-Lin
authors.initials: RL
authors.lastname: Wang
authors.forename: Jian-Long
authors.initials: JL
authors.lastname: Xiao
authors.forename: Yu-Jen
authors.initials: YJ
authors.lastname: Chang
authors.forename: Ji-Yen
authors.initials: JY
authors.lastname: Cheng
authors.forename: Yun-Ru
authors.initials: YR
authors.lastname: Chen
authors.forename: Chau-Hwang
authors.initials: CH
authors.lastname: Lee
mesh_terms: D016229:Amyloid beta-Peptides / Q000737:chemistry* / Q000378:metabolism; D000818:Animals; D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000737:chemistry* / Q000378:metabolism / Q000528:radiation effects; D004560:Electricity; D051379:Mice; D046529:Microscopy, Electron, Transmission; D061848:Optical Imaging / Q000379:methods*; D010446:Peptide Fragments / Q000737:chemistry* / Q000378:metabolism
chemical_list: D016229:Amyloid beta-Peptides; D010446:Peptide Fragments; C075222:amyloid beta-protein (1-42)