title: Xyolide, a bioactive nonenolide from an Amazonian endophytic fungus,Xylaria feejeensis.
abstract: Endophytes isolated from tropical plants represent a largely untapped reservoir of bioactive secondary metabolites. We screened a library of fungal endophyte extracts for inhibition of the plant pathogen,Pythium ultimum, and purified an active compound using bioassay-guided fractionation. A new nonenolide, (4S,7S,8S,9R)-4-O-succinyl-7,8-dihydroxy-9-heptyl-nonen-9-olide, was isolated and named xyolide. The structure was elucidated by a combination of 1D and 2D NMR methods and the absolute configuration was determined by exciton-coupled circular dichroism. The MIC of xyolide againstP. ultimumwas 425 µM.
authors.affiliation: Department of Molecular, Cellular, and Developmental Biology, Yale University, P.O. Box 208103, KBT 908 New Haven, CT, 06520 USA.
authors.forename: Ezra G
authors.initials: EG
authors.lastname: Baraban
authors.forename: Jesse B
authors.initials: JB
authors.lastname: Morin
authors.forename: Gillian M
authors.initials: GM
authors.lastname: Phillips
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Phillips
authors.forename: Scott A
authors.initials: SA
authors.lastname: Strobel
authors.forename: Jo
authors.initials: J
authors.lastname: Handelsman
keywords: Fungal endophyte; Natural product; Nonenolide; Pythium ultimum
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