title: MicroRNAs: new candidates for the regulation of the human cumulus-oocyte complex.
abstract: OBJECTIVE
  What is the expression pattern of microRNAs (miRNAs) in human cumulus-oocyte complexes (COCs)?
  
  
  CONCLUSIONS
  Several miRNAs are enriched in cumulus cells (CCs) or oocytes, and are predicted to target genes involved in biological functions of the COC.
  
  
  BACKGROUND
  The transcriptional profiles of human MII oocytes and the surrounding CCs are known. However, very limited data are available about post-transcriptional regulators, such as miRNAs. This is the first study focussing on the identification and quantification of small RNAs, including miRNAs, in human oocytes and CCs using a deep-sequencing approach.
  
  
  METHODS
  MII oocytes and CCs were collected from women who underwent IVF.
  
  
  METHODS
  Using the Illumina/deep-sequencing technology, we analyzed the small RNAome of pooled MII oocytes (n = 24) and CC samples (n = 20). The mRNA targets of CC and MII oocyte miRNAs were identified using in silico prediction algorithms. Using oligonucleotide microarrays, genome-wide gene expression was studied in oocytes (10 pools of 19 ± 3 oocytes/each) and 10 individual CC samples. TaqMan miRNA assays were used to confirm the sequencing results in independent pools of MII oocytes (3 pools of 8 ± 3 oocytes/each) and CC samples (3 pools of 7 ± 3 CCs/each). The functional role of one miRNA, MIR23a, was assessed in primary cultures of human CCs.
  
  
  RESULTS
  Deep sequencing of small RNAs yielded more than 1 million raw reads. By mapping reads with a single location to the human genome, known miRNAs that were abundant in MII oocytes (MIR184, MIR100 and MIR10A) or CCs (MIR29a, MIR30d, MIR21, MIR93, MIR320a, MIR125a and the LET7 family) were identified. Predicted target genes of the oocyte miRNAs were associated with the regulation of transcription and cell cycle, whereas genes targeted by CC miRNAs were involved in extracellular matrix and apoptosis. Comparison of the predicted miRNA target genes and mRNA microarray data resulted in a list of 224 target genes that were differentially expressed in MII oocytes and CCs, including PTGS2, CTGF and BMPR1B that are important for cumulus-oocyte communication. Functional analysis using primary CC cultures revealed that BCL2 and CYP19A1 mRNA levels were decreased upon MIR23a overexpression.
  
  
  CONCLUSIONS
  Only known miRNAs were investigated in the present study on COCs. Moreover, the source of the material is MII oocytes that failed to fertilize.
  
  
  CONCLUSIONS
  The present findings suggest that miRNA could play a role in the regulation of the oocyte and CC crosstalk.
  
  
  BACKGROUND
  This work was partially supported by a grant from Ferring Pharmaceuticals. The authors of the study have no conflict of interest to report.
  
  
  BACKGROUND
  Not applicable.
authors.affiliation: Université Montpellier 1, UFR de Médecine, Montpellier, France.
authors.forename: S
authors.initials: S
authors.lastname: Assou
authors.forename: T
authors.initials: T
authors.lastname: Al-edani
authors.forename: D
authors.initials: D
authors.lastname: Haouzi
authors.forename: N
authors.initials: N
authors.lastname: Philippe
authors.forename: C-H
authors.initials: CH
authors.lastname: Lecellier
authors.forename: D
authors.initials: D
authors.lastname: Piquemal
authors.forename: T
authors.initials: T
authors.lastname: Commes
authors.forename: O
authors.initials: O
authors.lastname: Aït-Ahmed
authors.forename: H
authors.initials: H
authors.lastname: Dechaud
authors.forename: S
authors.initials: S
authors.lastname: Hamamah
mesh_terms: D054885:Cumulus Cells / Q000378:metabolism / Q000502:physiology; D005260:Female; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006801:Humans; D035683:MicroRNAs / Q000378:metabolism* / Q000502:physiology; D009865:Oocytes / Q000378:metabolism / Q000502:physiology; D017423:Sequence Analysis, RNA
chemical_list: D035683:MicroRNAs
keywords: MicroRNAs; cumulus cells; deep sequencing; microarray; oocyte