title: GATA factor switching from GATA2 to GATA1 contributes to erythroid differentiation.
abstract: Transcription factor GATA2 is highly expressed in hematopoietic stem cells and progenitors, whereas its expression declines after erythroid commitment of progenitors. In contrast, the start of GATA1 expression coincides with the erythroid commitment and increases along with the erythroid differentiation. We refer this dynamic transition of GATA factor expression to as the 'GATA factor switching'. Here, we examined contribution of the GATA factor switching to the erythroid differentiation. In Gata1-knockdown embryos that concomitantly express Gata2-GFP reporter, high-level expression of GFP reporter was detected in accumulated immature hematopoietic cells with impaired differentiation, demonstrating that GATA1 represses Gata2 gene expression in hematopoietic progenitors in vivo. We have conducted chromatin immunoprecipitation (ChIP) on microarray analyses of GATA2 and GATA1, and results indicate that the GATA1-binding sites widely overlap with the sites pre-occupied by GATA2 before the GATA1 expression. Importantly, erythroid genes harboring GATA boxes bound by both GATA1 and GATA2 tend to be expressed in immature erythroid cells, whereas those harboring GATA boxes to which GATA1 binds highly but GATA2 binds only weakly are important for the mature erythroid cell function. Our results thus support the contention that preceding binding of GATA2 helps the following binding of GATA1 and thereby secures smooth expression of the transient-phase genes.
authors.affiliation: Department of Medical Biochemistry, Tohoku University Graduate School of Medicine, Sendai, 980-8575, Japan; Center for Radioisotope Sciences, Tohoku University Graduate School of Medicine, Sendai, 980-8575, Japan; Department of Molecular Hematology, Tohoku University Graduate School of Medicine, Sendai, 980-8575, Japan.
authors.forename: Mikiko
authors.initials: M
authors.lastname: Suzuki
authors.forename: Maki
authors.initials: M
authors.lastname: Kobayashi-Osaki
authors.forename: Shuichi
authors.initials: S
authors.lastname: Tsutsumi
authors.forename: Xiaoqing
authors.initials: X
authors.lastname: Pan
authors.forename: Shin'ya
authors.initials: S
authors.lastname: Ohmori
authors.forename: Jun
authors.initials: J
authors.lastname: Takai
authors.forename: Takashi
authors.initials: T
authors.lastname: Moriguchi
authors.forename: Osamu
authors.initials: O
authors.lastname: Ohneda
authors.forename: Kinuko
authors.initials: K
authors.lastname: Ohneda
authors.forename: Ritsuko
authors.initials: R
authors.lastname: Shimizu
authors.forename: Yasuharu
authors.initials: Y
authors.lastname: Kanki
authors.forename: Tatsuhiko
authors.initials: T
authors.lastname: Kodama
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Aburatani
authors.forename: Masayuki
authors.initials: M
authors.lastname: Yamamoto
mesh_terms: D000818:Animals; D001665:Binding Sites; D002454:Cell Differentiation; D041905:Erythroid Cells / Q000166:cytology* / Q000378:metabolism; D004920:Erythropoiesis / Q000502:physiology*; D050982:GATA1 Transcription Factor / Q000235:genetics* / Q000378:metabolism; D050989:GATA2 Transcription Factor / Q000235:genetics* / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000378:metabolism; D051379:Mice; D008822:Mice, Transgenic
chemical_list: D050982:GATA1 Transcription Factor; D050989:GATA2 Transcription Factor