title: Canine gastrointestinal pythiosis treatment by combined antifungal and immunotherapy and review of published studies.
abstract: Pythium insidiosum is an oomycete, a fungal like microorganism, which infects mammals, causing pythiosis in animals and humans, especially in tropical and subtropical regions around the world. The treatment for this infection is very difficult, and therapeutic options commonly comprise surgery, immunotherapy and antimicrobial drugs. The present report describes the clinical healing of a dog with gastrointestinal pythiosis by treatment with a combination of antifungals and immunotherapy, as well as reviews the cases reported in the literature that used some type of therapy for canine pythiosis. A 2.5-year-old male beagle initially showed sporadic vomiting episodes, and this symptom became more frequent 5 months after the onset of clinical signs. Celiotomy procedure found thickness of the stomach wall extending to the pylorus and duodenum. A biopsy was performed, and the diagnosis of pythiosis was made by mycological, histopathological analyses and molecular identification. Therapy was based on an association of terbinafine plus itraconazole during 12 months and immunotherapy for 2.5 months. The healing of the dog reported here allows us to propose the use of immunotherapy associated with antifungal therapy to treat canine gastrointestinal pythiosis. However, additional studies should be performed on a larger number of patients to establish a standard treatment protocol for canine pythiosis.
authors.affiliation: Laboratório de Micologia, Departamento de Microbiologia e Parasitologia, Instituto de Biologia, Universidade Federal de Pelotas (UFPel), Prédio 18, Sala 14, Campus Capão do Leão, Pelotas, RS, 96010-900, Brazil, danielabrayer@gmail.com.
authors.forename: Daniela I B
authors.initials: DI
authors.lastname: Pereira
authors.forename: Sônia A
authors.initials: SA
authors.lastname: Botton
authors.forename: Maria I
authors.initials: MI
authors.lastname: Azevedo
authors.forename: Marco A A
authors.initials: MA
authors.lastname: Motta
authors.forename: Raulene R
authors.initials: RR
authors.lastname: Lobo
authors.forename: Mauro P
authors.initials: MP
authors.lastname: Soares
authors.forename: Anelise O S
authors.initials: AO
authors.lastname: Fonseca
authors.forename: Francielli P K
authors.initials: FP
authors.lastname: Jesus
authors.forename: Sydney H
authors.initials: SH
authors.lastname: Alves
authors.forename: Janio M
authors.initials: JM
authors.lastname: Santurio
mesh_terms: D000818:Animals; D000935:Antifungal Agents / Q000008:administration & dosage*; D001706:Biopsy; D004283:Dog Diseases / Q000188:drug therapy*; D004285:Dogs; D004359:Drug Therapy, Combination / Q000379:methods; D004386:Duodenum / Q000473:pathology; D005767:Gastrointestinal Diseases / Q000188:drug therapy / Q000662:veterinary*; D006651:Histocytochemistry; D007155:Immunologic Factors / Q000008:administration & dosage*; D017964:Itraconazole / Q000008:administration & dosage; D008828:Microbiological Techniques; D009281:Naphthalenes / Q000008:administration & dosage; D058968:Pythiosis / Q000188:drug therapy / Q000662:veterinary*; D013270:Stomach / Q000473:pathology; D000077291:Terbinafine; D016896:Treatment Outcome
chemical_list: D000935:Antifungal Agents; D007155:Immunologic Factors; D009281:Naphthalenes; D017964:Itraconazole; D000077291:Terbinafine
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