title: Development of an ELISA for Quantitative Detection of Immunoglobulin G (IgG) and IgA Antibodies to Helicobacter pylori for Use in Korean Patients with H. pylori-Associated Diseases.
abstract: OBJECTIVE
  We aimed to develop a quantitative enzyme-linked immunosorbent assay (ELISA) using whole-cell lysates of Helicobacter pylori 51 and to investigate its validity.
  
  
  METHODS
  Data from 300 plates were obtained by two different operators. Standard sera were used to make a standard curve to analyze the quantity of anti-H. pylori immunoglobulin G (IgG) and IgA antibody. We obtained reproducible data with fewer dilutions of samples by the addition of serially diluted standard serum to each ELISA plate. To evaluate the validity of this ELISA, the 114 H. pylori-positive and -negative subjects were stratified into four age groups, i.e., 0 to 4, 5 to 9, 10 to 15, and 20 to 29 years, before testing.
  
  
  RESULTS
  The mean IgG-antibody titers in H. pylori-positive and -negative subjects were 1,766.4 IU/mL and 654.3 IU/mL (p<0.001). The mean IgA-antibody titers in H. pylori-positive and -negative subjects were 350.1 IU/mL and 193.5 IU/mL (p<0.001). Anti-H. pylori IgG and IgA titers in the four age groups were higher in H. pylori-positive subjects than in H. pylori-negative subjects (p<0.05).
  
  
  CONCLUSIONS
  Using the current ELISA based on whole-cell lysates of H. pylori 51, reliable anti-H. pylori antibody titers were obtained regardless of the subject's age.
authors.affiliation: Department of Pediatrics, Institute of Health Sciences, Gyeongsang National University School of Medicine, Jinju, Korea.
authors.forename: Ji-Hyun
authors.initials: JH
authors.lastname: Seo
authors.forename: Jin-Su
authors.initials: JS
authors.lastname: Jun
authors.forename: Hee-Shang
authors.initials: HS
authors.lastname: Youn
authors.forename: Jung Sook
authors.initials: JS
authors.lastname: Yeom
authors.forename: Ji Sook
authors.initials: JS
authors.lastname: Park
authors.forename: Chan-Hoo
authors.initials: CH
authors.lastname: Park
authors.forename: Hyang-Ok
authors.initials: HO
authors.lastname: Woo
authors.forename: Woo-Kon
authors.initials: WK
authors.lastname: Lee
authors.forename: Myung-Je
authors.initials: MJ
authors.lastname: Cho
authors.forename: Kwang-Ho
authors.initials: KH
authors.lastname: Rhee
keywords: Antibodies; Enzyme-linked immunosorbent assay; Helicobacter pylori
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