title: CMTM3 is frequently reduced in clear cell renal cell carcinoma and exhibits tumor suppressor activities.
abstract: OBJECTIVE
  CKLF-like MARVEL transmembrane domain containing member 3 (CMTM3) is silenced in many kinds of cancers and inhibits tumor cells growth. We investigated the expression and role of CMTM3 in clear cell renal cell carcinoma (ccRCC).
  
  
  METHODS
  The expression of CMTM3 was detected in ccRCC tissue microarray, specimens, and cell lines by immunohistochemistry, quantitative real-time polymerase chain reaction (qRT-PCR) and western blot, respectively. After transfected with CMTM3 plasmid or vector, the proliferation and migration of ccRCC 786-0 cells were determined by MTT assay and transwell assay, respectively. Furthermore, the anchorage-independent growth of transfected cells was assessed using soft agar colony formation assay.
  
  
  RESULTS
  CMTM3 was down-regulated in 84 % (63/75) of ccRCC tissues and its expression had no correlation with the gender, age, clinical staging and histologic grade. CMTM3 protein was undetectable by western blot in most detected ccRCC specimens and two RCC cell lines (786-0 and ACHN). qRT-PCR analysis showed that CMTM3 mRNA was dramatically down-regulated in 40 ccRCC cancer tissues as compared with the paired adjacent normal ones. Restoration of CMTM3 significantly suppressed the anchorage-independent growth, proliferation and migration of 786-0 cells.
  
  
  CONCLUSIONS
  These results indicate that CMTM3 is significantly down-regulated in ccRCC and exerts remarkable tumor-suppressive functions in 786-0 cells. Reduction of CMTM3 expression may contribute to the pathogenesis of ccRCC and CMTM3 may be a potentially target for therapeutic strategy.
authors.affiliation: Department of Urology, Peking University People's Hospital, Beijing, 100044, People's Republic of China.
authors.forename: J
authors.initials: J
authors.lastname: Xie
authors.forename: Y
authors.initials: Y
authors.lastname: Yuan
authors.forename: Z
authors.initials: Z
authors.lastname: Liu
authors.forename: Y
authors.initials: Y
authors.lastname: Xiao
authors.forename: X
authors.initials: X
authors.lastname: Zhang
authors.forename: C
authors.initials: C
authors.lastname: Qin
authors.forename: Z
authors.initials: Z
authors.lastname: Sheng
authors.forename: T
authors.initials: T
authors.lastname: Xu
authors.forename: X
authors.initials: X
authors.lastname: Wang
mesh_terms: D015153:Blotting, Western; D002292:Carcinoma, Renal Cell / Q000378:metabolism*; D018925:Chemokines / Q000378:metabolism*; D015536:Down-Regulation; D005260:Female; D016147:Genes, Tumor Suppressor / Q000502:physiology*; D006801:Humans; D007150:Immunohistochemistry; D007680:Kidney Neoplasms / Q000378:metabolism*; D062792:MARVEL Domain-Containing Proteins / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D060888:Real-Time Polymerase Chain Reaction; D046888:Tissue Array Analysis
chemical_list: C515016:CMTM3 protein, human; D018925:Chemokines; D062792:MARVEL Domain-Containing Proteins
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