title: Imaging mouse cancer models in vivo using reporter transgenes.
abstract: Imaging mouse models of cancer with reporter transgenes has become a relatively common experimental approach in the laboratory, which allows noninvasive and longitudinal investigation of diverse aspects of tumor biology in vivo. Our goal here is to outline briefly the principles of the relevant imaging modalities, emphasizing particularly their strengths and weaknesses and what the researcher can expect in a practical sense from each of these techniques. Furthermore, we discuss how relatively subtle modifications in the way reporter transgene expression is regulated in the cell underpin the ability of reporter transgenes as a whole to provide readouts on such varied aspects of tumor biology in vivo.
authors.affiliation: Department of Molecular Imaging, CRUK Cambridge Research Institute, Li Ka Shing Centre, Cambridge CB2 0RE, United Kingdom.
authors.forename: Scott K
authors.initials: SK
authors.lastname: Lyons
authors.forename: P Stephen
authors.initials: PS
authors.lastname: Patrick
authors.forename: Kevin M
authors.initials: KM
authors.lastname: Brindle
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D017930:Genes, Reporter; D007091:Image Processing, Computer-Assisted / Q000379:methods; D009369:Neoplasms / Q000473:pathology*; D061848:Optical Imaging / Q000379:methods*; D019076:Transgenes; D051598:Whole Body Imaging / Q000379:methods*