title: Ornithinimicrobium tianjinense sp. nov., isolated from a recirculating aquaculture system.
abstract: A Gram-positive, strictly aerobic and heterotrophic, non-spore-forming actinobacterium (strain B2(T)) isolated from a recirculating aquaculture system was studied for its taxonomic position. Strain B2(T) formed a rudimentary substrate-mycelium that fragmented into short rod-shaped to coccoid cells (0.5 µm×0.5-2.2 µm or 0.5-1.0 µm in diameter). Colonies were yellow, smooth, circular and 1.5-2.0 mm in diameter after incubation on TSA for 3 days at 30 °C. Strain B2(T) grew at 20-40 °C (optimal, 30 °C) and pH 5.5-9.5 (optimal, 6.5-7.0) and in the presence of 0-9% (w/v) NaCl (optimal, 1%). The predominant menaquinone of strain B2(T) was MK-8(H4). The cell-wall peptidoglycan of strain B2(T) contained the amino acids ornithine, glutamic acid, alanine, glycine and aspartic acid. The major polar lipids were phosphatidylglycerol and diphosphatidylglycerol. The major fatty acids were iso-C15 : 0, iso-C16 : 0 and summed feature 9. Its DNA G+C content was 68.3 mol% (Tm). Analysis of 16S rRNA gene sequences indicated that strain B2(T) was related phylogenetically to members of the genus Ornithinimicrobium with highest similarity (96.6 %) to Ornithinimicrobium kibberense DSM 17687(T), followed by Ornithinimicrobium humiphilum DSM 12362(T) (96.3 %), Ornithinimicrobium pekingense LW6(T) (96.2%) and Ornithinimicrobium murale 01-Gi-040(T) (94.8%). On basis of phenotypic, chemotaxonomic and phylogenetic data, it was concluded that strain B2(T) represents a novel species of the genus Ornithinimicrobium, for which the name Ornithinimicrobium tianjinense sp. nov. is proposed. The type strain is B2(T) ( =CGMCC 1.12160(T) =JCM 18464(T)).
authors.affiliation: University of Chinese Academy of Sciences, Beijing 100049, PR China.|State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
authors.forename: Liang-Zi
authors.initials: LZ
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Institute of Oceanology, Chinese Academy of Sciences, Qingdao, PR China.
authors.forename: Zhu
authors.initials: Z
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
authors.forename: Hong-Can
authors.initials: HC
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
authors.forename: Yu-Guang
authors.initials: YG
authors.lastname: Zhou
authors.affiliation: State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
authors.forename: Zhi-Pei
authors.initials: ZP
authors.lastname: Liu
mesh_terms: D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D017756:Aquaculture; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D008969:Molecular Sequence Data; D010457:Peptidoglycan / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry; D014871:Water Microbiology
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010457:Peptidoglycan; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C024223:vitamin MK 8