title: A simple mutagenesis using natural competence in Tannerella forsythia.
abstract: We report the discovery of natural competence in Tannerella forsythia and its application to targeted chromosomal mutagenesis. Keeping T. forsythia in a biofilm throughout the procedure allowed efficient DNA uptake and allelic replacement. This simple method is cost-effective and reproducible compared with the conventional protocols using broth culture and electroporation.
authors.affiliation: Department of Removable Prosthodontics, School of Dentistry, Aichi Gakuin University, 2-11 Suemori-dori, Chikusa-ku, Nagoya 464-8651, Japan. niski@dpc.agu.ac.jp
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Nishikawa
authors.forename: Yoshinobu
authors.initials: Y
authors.lastname: Tanaka
mesh_terms: D041963:Bacteroidetes / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D018441:Biofilms; D018929:Cell Culture Techniques; D004247:DNA / Q000378:metabolism; D005798:Genes, Bacterial; D008967:Molecular Biology / Q000379:methods; D016296:Mutagenesis; D010957:Plasmids; D014169:Transformation, Bacterial
chemical_list: D004247:DNA
keywords: Mutagenesis; Natural competence; Tannerella forsythia; Transformation