title: Protection of shrimp Penaeus monodon from WSSV infection using antisense constructs.
abstract: White spot syndrome caused by white spot syndrome virus (WSSV) is one of the most threatening diseases of shrimp culture industry. Previous studies have successfully demonstrated the use of DNA- and RNA-based vaccines to protect WSSV infection in shrimp. In the present study, we have explored the protective efficacy of antisense constructs directed against WSSV proteins, VP24, and VP28, thymidylate synthase (TS), and ribonucleotide reductase-2 (RR2) under the control of endogenous shrimp histone-3 (H3) or penaedin (Pn) promoter. Several antisense constructs were generated by inserting VP24 (pH3-VP24, pPn-VP24), VP28 (pH3-VP28, pPn-VP28), TS (pH3-TS, pPn-TS), and RR2 (pH3-RR2) in antisense orientation. These constructs were tested for their protective potential in WSSV infected cell cultures, and their effect on reduction of the viral load was assessed. A robust reduction in WSSV copy number was observed upon transfection of antisense constructs in hemocyte cultures derived from Penaeus monodon and Scylla serrata. When tested in vivo, antisense constructs offered a strong protection in WSSV challenged P. monodon. Constructs expressing antisense VP24 and VP28 provided the best protection (up to 90 % survivability) with a corresponding decrease in the viral load. Our work demonstrates that shrimp treated with antisense constructs present an efficient control strategy for combating WSSV infection in shrimp aquaculture.
authors.affiliation: Centre for Cellular and Molecular Biology, Council of Scientific and Industrial Research (CSIR), E405-East Wing 3rd Floor, CCMB, Uppal Road, Hyderabad, 500007, India.
authors.forename: Sajad
authors.initials: S
authors.lastname: Ahanger
authors.forename: Supriyanka
authors.initials: S
authors.lastname: Sandaka
authors.forename: Deepika
authors.initials: D
authors.lastname: Ananad
authors.forename: Madhu K
authors.initials: MK
authors.lastname: Mani
authors.forename: Ravinder
authors.initials: R
authors.lastname: Kondadhasula
authors.forename: Chandra Sekhar
authors.initials: CS
authors.lastname: Reddy
authors.forename: Makesh
authors.initials: M
authors.lastname: Marappan
authors.forename: Rajendran K
authors.initials: RK
authors.lastname: Valappil
authors.forename: Kshitish C
authors.initials: KC
authors.lastname: Majumdar
authors.forename: Rakesh K
authors.initials: RK
authors.lastname: Mishra
mesh_terms: D000818:Animals; D016375:Antisense Elements (Genetics) / Q000235:genetics / Q000276:immunology*; D017756:Aquaculture / Q000379:methods*; D016466:CHO Cells; D018929:Cell Culture Techniques; D006224:Cricetinae; D003412:Cricetulus; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006434:Hemocytes / Q000276:immunology / Q000821:virology; D033561:Penaeidae / Q000276:immunology* / Q000821:virology*; D011401:Promoter Regions, Genetic / Q000235:genetics; D012264:Ribonucleotide Reductases / Q000235:genetics / Q000276:immunology*; D013940:Thymidylate Synthase / Q000235:genetics / Q000276:immunology; D013997:Time Factors; D019562:Viral Load / Q000276:immunology; D014765:Viral Vaccines / Q000276:immunology*; D046848:White spot syndrome virus 1 / Q000276:immunology*
chemical_list: D016375:Antisense Elements (Genetics); D014765:Viral Vaccines; D049452:Green Fluorescent Proteins; D012264:Ribonucleotide Reductases; D013940:Thymidylate Synthase
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