title: Effects of PTH (1-84) on bone quality in a validated model of osteoporosis due to androgenic deprivation.
abstract: OBJECTIVE
  The purpose of this study was to evaluate the effect of parathyroid hormone (PTH) (1-84) in a model of male osteoporosis induced by orchidectomy in rats.
  
  
  METHODS
  Six-month-old Wistar rats were used as follows: SHAM (simulated orchidectomy), orchidectomized (ORX), ORX + PTH1 (ORX and treated with 10 µg/Kg/d of PTH 1-84) and ORX + PTH2 (ORX and treated with 50 µg/Kg/d of PTH 1-84) over 3 months, with treatment beginning three months after orchidectomy.
  
  
  RESULTS
  Orchidectomy resulted in a decreased of femoral and lumbar bone mineral density (BMD), a worsening of trabecular and cortical microarchitecture and a decrease in biomechanical properties. Both doses of PTH (1-84) partially (low dose) or totally (high dose) restored the ORX-induced changes. Serum C-telopeptide of type I collagen/5b isoenzyme of tartrate-resistant acid phosphatase (CTX/TRAP) resorption index increased after orchidectomy. Osteocalcin (bone Gla protein; BGP) levels were not affected by orchidectomy. PTH (1-84) treatment did not produce any changes in the levels of CTX/TRAP with respect to the ORX group. BGP levels increased with PTH treatment.
  
  
  CONCLUSIONS
  PTH (1-84) is able to restore the adverse effects of orchidectomy on bone as measured by BMD, microstructural and biomechanical properties and bone remodeling markers.
authors.affiliation: Department of Biochemical Research, Instituto de Investigación Sanitaria Fundación Jiménez Díaz , Madrid , Spain .
authors.forename: Marta
authors.initials: M
authors.lastname: Martín-Fernández
authors.forename: Elena
authors.initials: E
authors.lastname: Martínez
authors.forename: Manuel
authors.initials: M
authors.lastname: Díaz-Curiel
authors.forename: David
authors.initials: D
authors.lastname: Guede
authors.forename: José Ramón
authors.initials: JR
authors.lastname: Caeiro
authors.forename: Concepción
authors.initials: C
authors.lastname: De la Piedra
mesh_terms: D015502:Absorptiometry, Photon; D000135:Acid Phosphatase / Q000097:blood; D000818:Animals; D015415:Biomarkers / Q000097:blood; D001696:Biomechanical Phenomena; D015519:Bone Density / Q000187:drug effects; D024042:Collagen Type I / Q000097:blood; D004797:Enzyme-Linked Immunosorbent Assay; D007527:Isoenzymes / Q000097:blood; D008297:Male; D009919:Orchiectomy; D015675:Osteocalcin / Q000097:blood; D010024:Osteoporosis / Q000000981:diagnostic imaging / Q000188:drug therapy*; D010281:Parathyroid Hormone / Q000494:pharmacology*; D010455:Peptides / Q000097:blood; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D000071681:Tartrate-Resistant Acid Phosphatase; D055114:X-Ray Microtomography
chemical_list: D015415:Biomarkers; D024042:Collagen Type I; D007527:Isoenzymes; D010281:Parathyroid Hormone; D010455:Peptides; C052929:collagen type I trimeric cross-linked peptide; D015675:Osteocalcin; D000135:Acid Phosphatase; D000071681:Tartrate-Resistant Acid Phosphatase