title: Early induction of uncoupling protein-2 in pulmonary macrophages in hyperoxia-associated lung injury.
abstract: BACKGROUND
  High concentrations of inspired oxygen contribute to the pathogenesis of neonatal bronchopulmonary dysplasia and adult acute respiratory distress syndrome. Animal models of hyperoxia-associated lung injury (HALI) are characterized by enhanced generation of reactive oxygen species (ROS) and an adaptive antioxidant response. ROS contribute to pathogenesis, partly through enhancing pro-inflammatory activity in macrophages. Uncoupling protein-2 (UCP2) is an inner mitochondrial membrane protein whose expression lowers mitochondrial superoxide (O₂ⁱ⁻) production. UCP2, therefore, has potential to contribute to antioxidant response. It is inducible in macrophages.
  
  
  OBJECTIVE
  We hypothesized that induction of UCP2 occurred in response to pulmonary hyperoxia in vivo and that expression localized to pulmonary macrophages. We then investigated mechanisms of UCP2 regulation in hyperoxia-exposed macrophages in vitro and correlated changing UCP2 expression with mitochondrial membrane potential (Δψm) and O₂ⁱ⁻ production.
  
  
  RESULTS
  UCP2 is induced in lungs of mice within 1 h of hyperoxia exposure. Induction occurs in pulmonary alveolar macrophages in vivo, and can be replicated in vitro in isolated macrophages. UCP2 mRNA does not change. UCP2 increases quickly after the first hyperoxia-induced burst of mitochondrial O₂ⁱ⁻ generation. Suppression of Δψm and mitochondrial O₂ⁱ⁻ production follow and persist while UCP2 is elevated.
  
  
  CONCLUSIONS
  Induction of UCP2 is an early response to hyperoxia in pulmonary macrophages. The mechanism is post-transcriptional. UCP2 induction follows a transient rise in mitochondrial ROS generation. The subsequent falls in Δψm and mitochondrial O₂ⁱ⁻ support the notion that regulable UCP2 expression in macrophages acts to contain mitochondrial ROS generation. That, in turn, may limit inappropriate pro-inflammatory activation in HALI.
authors.affiliation: School of Medicine & Pharmacology, University of Western Australia, Crawley, Western Australia, Australia.
authors.forename: James H
authors.initials: JH
authors.lastname: Steer
authors.forename: Tracy S
authors.initials: TS
authors.lastname: Mann
authors.forename: Susan Z Y
authors.initials: SZ
authors.lastname: Lo
authors.forename: Julia J
authors.initials: JJ
authors.lastname: Inglis
authors.forename: Huey S
authors.initials: HS
authors.lastname: Yap
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Henry
authors.forename: David A
authors.initials: DA
authors.lastname: Joyce
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology; D002460:Cell Line; D002478:Cells, Cultured; D018496:Hyperoxia / Q000150:complications / Q000378:metabolism*; D007473:Ion Channels / Q000235:genetics / Q000378:metabolism*; D008168:Lung / Q000378:metabolism; D055370:Lung Injury / Q000209:etiology / Q000378:metabolism*; D008264:Macrophages / Q000502:physiology*; D008297:Male; D053078:Membrane Potential, Mitochondrial; D051379:Mice; D008807:Mice, Inbred BALB C; D024101:Mitochondrial Proteins / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D013481:Superoxides / Q000378:metabolism; D000071246:Uncoupling Protein 2
chemical_list: D007473:Ion Channels; D024101:Mitochondrial Proteins; D012333:RNA, Messenger; C000604390:Ucp2 protein, mouse; D000071246:Uncoupling Protein 2; D013481:Superoxides; D000255:Adenosine Triphosphate