title: GCSF-R expression in myelodysplastic and myeloproliferative disorders and blast dysmaturation in CML.
abstract: OBJECTIVE
  To characterize granulocyte colony-stimulating factor receptor (CD114) expression in normal (n = 20), myelodysplastic (n = 34), and chronic myelogenous leukemia (CML; n = 5) bone marrow by flow cytometry.
  
  
  METHODS
  Clinical bone marrow samples were analyzed using CD33/CD114/CD34/CD117/CD45. CD114 density (mean fluorescence intensity) and cellular distribution were evaluated on early blasts (CD33-), late blasts (CD33+), promyelocytes, and granulocytes.
  
  
  RESULTS
  Normal CD114 acquisition occurred on early blasts, peaked on promyelocytes, and decreased on granulocytes. Forty percent of CD34+ blasts expressed CD114 and one-third were early blasts. In myelodysplastic syndromes, altered CD114 distribution was more informative than density changes. In CML, CD114 density was significantly decreased on early blasts and expression was essentially limited to late blasts. We observed a specific blast dysmaturation pattern in CML involving CD33, CD34, and CD114 that was 83% sensitive and 100% specific in initial diagnosis.
  
  
  CONCLUSIONS
  CD114 provides useful additional detail in phenotypic assessment of hematopoietic precursor maturation.
authors.affiliation: Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, NE 68198, USA.
authors.forename: Vidya Sagar
authors.initials: VS
authors.lastname: Hanumanthu
authors.forename: Samuel J
authors.initials: SJ
authors.lastname: Pirruccello
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D018952:Antigens, CD34 / Q000378:metabolism; D014408:Biomarkers, Tumor / Q000378:metabolism; D001853:Bone Marrow / Q000276:immunology / Q000378:metabolism*; D005260:Female; D005434:Flow Cytometry; D006098:Granulocytes / Q000276:immunology / Q000378:metabolism; D006801:Humans; D007962:Leukocytes / Q000276:immunology / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D009190:Myelodysplastic Syndromes / Q000276:immunology / Q000378:metabolism*; D009196:Myeloproliferative Disorders / Q000276:immunology / Q000378:metabolism*; D016188:Receptors, Granulocyte Colony-Stimulating Factor / Q000378:metabolism*; D063268:Sialic Acid Binding Ig-like Lectin 3 / Q000378:metabolism
chemical_list: D018952:Antigens, CD34; D014408:Biomarkers, Tumor; D016188:Receptors, Granulocyte Colony-Stimulating Factor; D063268:Sialic Acid Binding Ig-like Lectin 3
keywords: CD114; Chronic myelogenous leukemia; Flow cytometry; GCSF-R; Myelodysplastic syndrome