title: A mechanism for localized dynamics-driven affinity regulation of the binding of von Willebrand factor to platelet glycoprotein Ibα.
abstract: Binding of the A1 domain of von Willebrand factor (vWF) to glycoprotein Ibα (GPIbα) results in platelet adhesion, activation, and aggregation that initiates primary hemostasis. Both the elevated shear stress and the mutations associated with type 2B von Willebrand disease enhance the interaction between A1 and GPIbα. Through molecular dynamics simulations for wild-type vWF-A1 and its eight gain of function mutants (R543Q, I546V, ΔSS, etc.), we found that the gain of function mutations destabilize the N-terminal arm, increase a clock pendulum-like movement of the α2-helix, and turn a closed A1 conformation into a partially open one favoring binding to GPIbα. The residue Arg(578) at the α2-helix behaves as a pivot in the destabilization of the N-terminal arm and a consequent dynamic change of the α2-helix. These results suggest a localized dynamics-driven affinity regulation mechanism for vWF-GPIbα interaction. Allosteric drugs controlling this intrinsic protein dynamics may be effective in blocking the GPIb-vWF interaction.
authors.affiliation: From the Institute of Biomechanics, School of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510006, China.
authors.forename: Guangjian
authors.initials: G
authors.lastname: Liu
authors.forename: Ying
authors.initials: Y
authors.lastname: Fang
authors.forename: Jianhua
authors.initials: J
authors.lastname: Wu
mesh_terms: D001792:Blood Platelets / Q000378:metabolism; D005786:Gene Expression Regulation; D006801:Humans; D006860:Hydrogen Bonding; D008024:Ligands; D008958:Models, Molecular; D056004:Molecular Dynamics Simulation; D009154:Mutation; D019038:Platelet Glycoprotein GPIb-IX Complex / Q000378:metabolism*; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D013927:Thrombosis / Q000378:metabolism; D014842:von Willebrand Diseases / Q000378:metabolism; D014841:von Willebrand Factor / Q000378:metabolism*
chemical_list: D008024:Ligands; D019038:Platelet Glycoprotein GPIb-IX Complex; D014841:von Willebrand Factor
keywords: Biophysics; GPIbα; Molecular Dynamics; Platelets; Protein Dynamics; Structural Biology; Thrombosis; von Willebrand Disease; von Willebrand Factor
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