title: Does tobramycin impregnation of allograft bone affect implant fixation? - an experimental study in 12 dogs.
abstract: Clinical trials have used antibiotic impregnated impacted bone allograft in revisions of infected arthroplasties. By this method high local antibiotic concentration and good control of infection was achieved. Toxicity studies, however, suggest that high local antibiotic concentration can impair osteoblast replication. We therefore asked whether impregnating morselized allograft bone with different quantities of tobramycin before impaction would impair implant fixation. We implanted three cylindrical (10 mm × 6 mm) porous-coated titanium implants into the distal femurs of 12 dogs. The implants were surrounded by a circumferential gap of 2.5 mm into which a standardized volume of morselized allograft bone, with or without tobramycin, was impacted. In each animal, the bone graft was impregnated with either 0 mg (control), 50 mg (low dose), or 200 mg (high dose) of tobramycin per 1 mL of bone graft. At the end of the 4 weeks experimental period, the implants with surrounding bone were evaluated by histomorphometric analysis and mechanical push-out test. We found no difference between the treatment groups regarding new bone formation, bone graft resorption, or implant fixation. There was, however, a tendency toward a decrease in implant fixation with higher tobramycin dose. The present study is unable to provide evidence on whether the use of topical tobramycin with allograft is safe or whether it indeed can impair implant fixation. The tendency toward an impaired implant fixation warrants further preclinical studies. Its current clinical use should be weighed against its possible positive effects on preventing infection in complicated revisions.
authors.affiliation: Orthopaedic Research Laboratory, Aarhus University Hospital, Aarhus, Denmark.
authors.forename: Jeppe
authors.initials: J
authors.lastname: Barckman
authors.forename: Jorgen
authors.initials: J
authors.lastname: Baas
authors.forename: Mette
authors.initials: M
authors.lastname: Sorensen
authors.forename: Jeppe
authors.initials: J
authors.lastname: Lange
authors.forename: Joan E
authors.initials: JE
authors.lastname: Bechtold
authors.forename: Kjeld
authors.initials: K
authors.lastname: Soballe
mesh_terms: D064591:Allografts; D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage* / Q000009:adverse effects*; D001696:Biomechanical Phenomena; D016025:Bone Transplantation / Q000379:methods*; D003692:Delayed-Action Preparations; D004285:Dogs; D005260:Female; D023421:Models, Animal; D016348:Osseointegration / Q000187:drug effects*; D019736:Prostheses and Implants; D011475:Prosthesis Failure; D016459:Prosthesis-Related Infections / Q000517:prevention & control; D012086:Reoperation / Q000379:methods; D014025:Titanium; D014031:Tobramycin / Q000008:administration & dosage* / Q000009:adverse effects*
chemical_list: D000900:Anti-Bacterial Agents; D003692:Delayed-Action Preparations; D014025:Titanium; D014031:Tobramycin
keywords: implant fixation; impregnation; morselized allograft bone; osseointegration; tobramycin
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