title: A novel 5-enolpyruvoylshikimate-3-phosphate (EPSP) synthase transgene for glyphosate resistance stimulates growth and fecundity in weedy rice (Oryza sativa) without herbicide.
abstract: Understanding evolutionary interactions among crops and weeds can facilitate effective weed management. For example, gene flow from crops to their wild or weedy relatives can lead to rapid evolution in recipient populations. In rice (Oryza sativa), transgenic herbicide resistance is expected to spread to conspecific weedy rice (Oryza sativa f. spontanea) via hybridization. Here, we studied fitness effects of transgenic over-expression of a native 5-enolpyruvoylshikimate-3-phosphate synthase (epsps) gene developed to confer glyphosate resistance in rice. Controlling for genetic background, we examined physiological traits and field performance of crop-weed hybrid lineages that segregated for the presence or absence of this novel epsps transgene. Surprisingly, we found that transgenic F2 crop-weed hybrids produced 48-125% more seeds per plant than nontransgenic controls in monoculture- and mixed-planting designs without glyphosate application. Transgenic plants also had greater EPSPS protein levels, tryptophan concentrations, photosynthetic rates, and per cent seed germination compared with nontransgenic controls. Our findings suggest that over-expression of a native rice epsps gene can lead to fitness advantages, even without exposure to glyphosate. We hypothesize that over-expressed epsps may be useful to breeders and, if deployed, could result in fitness benefits in weedy relatives following transgene introgression.
authors.affiliation: Ministry of Education Key Laboratory for Biodiversity and Ecological Engineering, Institute of Biodiversity Science, Fudan University, Handan Road 220, Shanghai, 200433, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.affiliation: Ministry of Education Key Laboratory for Biodiversity and Ecological Engineering, Institute of Biodiversity Science, Fudan University, Handan Road 220, Shanghai, 200433, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Xia
authors.affiliation: Ministry of Education Key Laboratory for Biodiversity and Ecological Engineering, Institute of Biodiversity Science, Fudan University, Handan Road 220, Shanghai, 200433, China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Yang
authors.affiliation: Ministry of Education Key Laboratory for Biodiversity and Ecological Engineering, Institute of Biodiversity Science, Fudan University, Handan Road 220, Shanghai, 200433, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Xu
authors.affiliation: Ministry of Education Key Laboratory for Biodiversity and Ecological Engineering, Institute of Biodiversity Science, Fudan University, Handan Road 220, Shanghai, 200433, China.
authors.forename: Hong Jiang
authors.initials: HJ
authors.lastname: Si
authors.affiliation: Ministry of Education Key Laboratory for Biodiversity and Ecological Engineering, Institute of Biodiversity Science, Fudan University, Handan Road 220, Shanghai, 200433, China.
authors.forename: Xing Xing
authors.initials: XX
authors.lastname: Cai
authors.affiliation: Fujian Province Key Laboratory of Genetic Engineering for Agriculture, Fujian Academy of Agricultural Sciences, Fuzhou, 350003, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Wang
authors.affiliation: Fujian Province Key Laboratory of Genetic Engineering for Agriculture, Fujian Academy of Agricultural Sciences, Fuzhou, 350003, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Su
authors.affiliation: Department of Evolution, Ecology, & Organismal Biology, Ohio State University, Columbus, OH, 43210-1293, USA.
authors.forename: Allison A
authors.initials: AA
authors.lastname: Snow
authors.affiliation: Ministry of Education Key Laboratory for Biodiversity and Ecological Engineering, Institute of Biodiversity Science, Fudan University, Handan Road 220, Shanghai, 200433, China.
authors.forename: Bao-Rong
authors.initials: BR
authors.lastname: Lu
mesh_terms: D051229:3-Phosphoshikimate 1-Carboxyvinyltransferase / Q000235:genetics* / Q000378:metabolism; D003433:Crosses, Genetic; D017343:Genes, Plant; D056084:Genetic Fitness; D018525:Germination / Q000235:genetics; D005998:Glycine / Q000031:analogs & derivatives; D006540:Herbicides; D006824:Hybridization, Genetic; D012275:Oryza / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D010788:Photosynthesis / Q000235:genetics; D057685:Plant Weeds; D030821:Plants, Genetically Modified; D012639:Seeds / Q000254:growth & development; D012765:Shikimic Acid / Q000031:analogs & derivatives* / Q000378:metabolism; D013045:Species Specificity; D019076:Transgenes; D014364:Tryptophan / Q000235:genetics / Q000378:metabolism; D000097797:Glyphosate
chemical_list: D051229:3-Phosphoshikimate 1-Carboxyvinyltransferase; D005998:Glycine; D006540:Herbicides; D012765:Shikimic Acid; D014364:Tryptophan; D000097797:Glyphosate; C058070:5-enolpyruvoylshikimate-3-phosphate
keywords: Oryza sativa; epsps; fitness; genetically engineered; glyphosate-resistant; introgression; risk assessment; weedy rice
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