title: Molecular identification of leishmania species using samples obtained from negative stained smears.
abstract: BACKGROUND
  Cutaneous Leishmaniasis (CL) is a parasitic skin disease. Diagnosis primarily is based on clinical signs and microscopic observation of parasite on direct stained smears or tissue sections. Sensitivity of direct smear is not as high as molecular methods. The aim of this study was to identify and characterize Leishmania species among the negative direct smears obtained from skin ulcers suspected to CL by PCR method.
  
  
  METHODS
  Among 81 patients with suspicious skin lesions to CL referred to the Parasitology lab, negative Giemsa stained smears were collected. DNA extraction performed by scraping stained smears, then PCR was performed.
  
  
  RESULTS
  Among the DNA extracted from smears, L. tropica was isolated from 9 (11.1%) of the smears and L.major was not isolated from any samples.
  
  
  CONCLUSIONS
  Direct microscopy on stained smears for diagnosis of leishmaniasis is not enough accurate. PCR is recommended for clinically suspected lesions with negative result of direct smear.
authors.affiliation: Department of Medical Parasitology and Mycology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
authors.forename: Ma
authors.initials: M
authors.lastname: Mohaghegh
authors.forename: A
authors.initials: A
authors.lastname: Fata
authors.forename: Gh
authors.initials: G
authors.lastname: Salehi
authors.forename: F
authors.initials: F
authors.lastname: Berenji
authors.forename: M Mousavi
authors.initials: MM
authors.lastname: Bazzaz
authors.forename: H
authors.initials: H
authors.lastname: Rafatpanah
authors.forename: M
authors.initials: M
authors.lastname: Parian
authors.forename: A
authors.initials: A
authors.lastname: Movahedi
keywords: Cutaneous Leishmaniasis; Direct smear; Leishmania tropica; PCR
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