title: The MFS-type efflux pump Flr1 induced by Yap1 promotes canthin-6-one resistance in yeast.
abstract: Screening for suppressors of canthin-6-one toxicity in yeast identified Yap1, a transcription factor involved in cell response to a broad range of injuries. Although canthin-6-one did not promote a significant oxidative stress, overexpression of YAP1 gene clearly increased resistance to this drug. We demonstrated that Yap1-mediated resistance involves the plasma membrane major-facilitator-superfamily efflux pump Flr1 but not the vacuolar ATP-binding-cassette transporter Ycf1. FLR1 overexpression was sufficient to reduce sensitivity to the drug, but strictly dependent on a functional YAP1 gene.
authors.affiliation: Institut de Physiologie et Biologie Cellulaires, CNRS FRE 3511, Université de Poitiers, Poitiers, France.
authors.forename: Camille
authors.initials: C
authors.lastname: Dejos
authors.forename: Matthieu
authors.initials: M
authors.lastname: Régnacq
authors.forename: Marianne
authors.initials: M
authors.lastname: Bernard
authors.forename: Pierre
authors.initials: P
authors.lastname: Voisin
authors.forename: Thierry
authors.initials: T
authors.lastname: Bergès
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000235:genetics / Q000378:metabolism; D002243:Carbolines / Q000494:pharmacology*; D002462:Cell Membrane / Q000187:drug effects; D025141:Drug Resistance, Fungal / Q000187:drug effects* / Q000235:genetics; D005659:Fungicides, Industrial / Q000494:pharmacology*; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects*; D026121:Indole Alkaloids / Q000494:pharmacology*; D027361:Organic Anion Transporters / Q000235:genetics* / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics* / Q000378:metabolism; D015398:Signal Transduction; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism
chemical_list: D018528:ATP-Binding Cassette Transporters; D002243:Carbolines; C118429:FLR1 protein, S cerevisiae; D005659:Fungicides, Industrial; D026121:Indole Alkaloids; D027361:Organic Anion Transporters; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; C086677:YAP1 protein, S cerevisiae; C089179:YCF1 protein, S cerevisiae; C453379:canthin-6-one
keywords: Canthin-6-one resistance; Membrane efflux pump; Saccharomyces cerevisiae; Transcription factor