title: Differential modulation of ROS signals and other mitochondrial parameters by the antioxidants MitoQ, resveratrol and curcumin in human adipocytes.
abstract: Mitochondrial reactive oxygen species (ROS) have been demonstrated to play an important role as signaling and regulating molecules in human adipocytes. In order to evaluate the differential modulating roles of antioxidants, we treated human adipocytes differentiated from human bone marrow-derived mesenchymal stem cells with MitoQ, resveratrol and curcumin. The effects on ROS, viability, mitochondrial respiration and intracellular ATP levels were examined. MitoQ lowered both oxidizing and reducing ROS. Resveratrol decreased reducing and curcumin oxidizing radicals only. All three substances slightly decreased state III respiration immediately after addition. After 24 h of treatment, MitoQ inhibited both basal and uncoupled oxygen consumption, whereas curcumin and resveratrol had no effect. Intracellular ATP levels were not altered. This demonstrates that MitoQ, resveratrol and curcumin exert potent modulating effects on ROS signaling in human adipocyte with marginal effects on metabolic parameters.
authors.affiliation: Laboratory of Endocrinology, Department of Biomedicine, University Hospital and University Children's Hospital .
authors.forename: Estelle
authors.initials: E
authors.lastname: Hirzel
authors.forename: Peter W
authors.initials: PW
authors.lastname: Lindinger
authors.forename: Swarna
authors.initials: S
authors.lastname: Maseneni
authors.forename: Maria
authors.initials: M
authors.lastname: Giese
authors.forename: Véronique Virginie
authors.initials: VV
authors.lastname: Rhein
authors.forename: Anne
authors.initials: A
authors.lastname: Eckert
authors.forename: Matthias
authors.initials: M
authors.lastname: Hoch
authors.forename: Stephan
authors.initials: S
authors.lastname: Krähenbühl
authors.forename: Alex N
authors.initials: AN
authors.lastname: Eberle
mesh_terms: D017667:Adipocytes / Q000166:cytology / Q000378:metabolism*; D000975:Antioxidants / Q000378:metabolism*; D001854:Bone Marrow Cells / Q000166:cytology; D002454:Cell Differentiation; D019069:Cell Respiration / Q000187:drug effects*; D003474:Curcumin / Q000494:pharmacology*; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D059630:Mesenchymal Stem Cells / Q000166:cytology; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D009943:Organophosphorus Compounds / Q000494:pharmacology; D010101:Oxygen Consumption / Q000187:drug effects; D017382:Reactive Oxygen Species / Q000378:metabolism; D000077185:Resveratrol; D013267:Stilbenes / Q000494:pharmacology*; D014451:Ubiquinone / Q000031:analogs & derivatives / Q000494:pharmacology
chemical_list: D000975:Antioxidants; D009943:Organophosphorus Compounds; D017382:Reactive Oxygen Species; D013267:Stilbenes; D014451:Ubiquinone; C429014:mitoquinone; D006861:Hydrogen Peroxide; D003474:Curcumin; D000077185:Resveratrol