title: Pyrosequence analysis of the hsp65 genes of nontuberculous mycobacterium communities in unchlorinated drinking water in the Netherlands.
abstract: Studies have shown that certain opportunistic pathogenic species of nontuberculous mycobacteria (NTM) can be present in distributed drinking water. However, detailed information about NTM population composition in drinking water is lacking. Therefore, NTM communities in unchlorinated drinking water from the distribution system of five treatment plants in the Netherlands were characterized using 454 pyrosequencing of the hsp65 gene. Results showed high diversities in unchlorinated drinking water, with up to 28 different NTM operational taxonomic units (OTUs) in a single sample. Each drinking water sample had a unique NTM community, and most (81.1%) OTUs were observed only once. One OTU was observed in 14 of 16 drinking water samples, indicating that this NTM species is well adapted to unchlorinated drinking water conditions. A clear influence of season, source type (groundwater, surface water), easily assimilable organic carbon (AOC) concentration, biofilm formation rate, and active biomass in treated water on the establishment of an NTM community in drinking water was not observed. Apparently, local conditions are more important for the development of a specific NTM community in the drinking water distribution system. A low (4.2%) number of hsp65 gene sequences showed more than 97% similarity to sequences of the opportunistic pathogens M. avium, M. genavense, and M. gordonae. However, most (95.8%) NTM hsp65 gene sequences were related to not-yet-described NTM species that have not been linked to disease, indicating that most NTM species in unchlorinated drinking water from distribution systems in the Netherlands have a low public health significance.
authors.affiliation: KWR Watercycle Research Institute, Nieuwegein, The Netherlands.
authors.forename: Paul W J J
authors.initials: PW
authors.lastname: van der Wielen
authors.forename: Leo
authors.initials: L
authors.lastname: Heijnen
authors.forename: Dick
authors.initials: D
authors.lastname: van der Kooij
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D044822:Biodiversity; D018834:Chaperonin 60 / Q000235:genetics*; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D060766:Drinking Water / Q000382:microbiology*; D014644:Genetic Variation; D005838:Genotype; D008969:Molecular Sequence Data; D009426:Netherlands; D009170:Nontuberculous Mycobacteria / Q000235:genetics*; D010802:Phylogeny; D017422:Sequence Analysis, DNA
chemical_list: D001426:Bacterial Proteins; D018834:Chaperonin 60; D004269:DNA, Bacterial; D060766:Drinking Water; C074119:heat-shock protein 65, Mycobacterium
references.citation: Thorax. 2009 Jun;64(6):502-6
references.pmid: 19213773
references.citation: Appl Environ Microbiol. 2009 Dec;75(23):7537-41
references.pmid: 19801464
references.citation: Oecologia. 1981 Sep;50(3):296-302
references.pmid: 28309044
references.citation: Appl Environ Microbiol. 2007 Oct;73(19):6201-7
references.pmid: 17675427
references.citation: Emerg Infect Dis. 2011 Mar;17(3):395-402
references.pmid: 21392429
references.citation: Appl Environ Microbiol. 2006 Sep;72(9):5864-9
references.pmid: 16957205
references.citation: Int J Med Microbiol. 2009 Apr;299(4):281-90
references.pmid: 18774336
references.citation: Clin Microbiol Rev. 1996 Apr;9(2):177-215
references.pmid: 8964035
references.citation: Appl Environ Microbiol. 2009 Aug;75(16):5363-72
references.pmid: 19581474
references.citation: Appl Environ Microbiol. 1999 Jun;65(6):2492-6
references.pmid: 10347032
references.citation: Science. 2005 Jan 7;307(5706):121-3
references.pmid: 15637281
references.citation: Zentralbl Bakteriol Orig B. 1973 Oct;158(2):161-9
references.pmid: 4779173
references.citation: Appl Environ Microbiol. 2001 Mar;67(3):1225-31
references.pmid: 11229914
references.citation: Appl Environ Microbiol. 2002 Nov;68(11):5318-25
references.pmid: 12406720
references.citation: Infect Control Hosp Epidemiol. 2004 Dec;25(12):1050-5
references.pmid: 15636291
references.citation: Water Res. 2004 Mar;38(6):1457-66
references.pmid: 15016522
references.citation: Appl Environ Microbiol. 2010 Sep;76(17):6017-9
references.pmid: 20639378
references.citation: Zentralbl Hyg Umweltmed. 1989 Jun;188(3-4):385-90
references.pmid: 2667558
references.citation: Appl Environ Microbiol. 2010 Jul;76(14):4876-81
references.pmid: 20511431
references.citation: Appl Environ Microbiol. 2001 Oct;67(10):4432-9
references.pmid: 11571139
references.citation: Tubercle. 1980 Mar;61(1):21-6
references.pmid: 7368320
references.citation: ISME J. 2013 Jun;7(6):1092-101
references.pmid: 23407313
references.citation: Am Rev Respir Dis. 1991 Nov;144(5):1153-9
references.pmid: 1952447
references.citation: Eur J Clin Microbiol Infect Dis. 2012 May;31(5):683-94
references.pmid: 21805195
references.citation: Appl Environ Microbiol. 1997 Feb;63(2):547-52
references.pmid: 16535511
references.citation: Appl Environ Microbiol. 2004 Apr;70(4):1973-81
references.pmid: 15066787
references.citation: Environ Sci Technol. 2012 Nov 6;46(21):11566-74
references.pmid: 23046164
references.citation: Appl Environ Microbiol. 2003 Nov;69(11):6946-8
references.pmid: 14602661
references.citation: Int J Syst Evol Microbiol. 2005 Jul;55(Pt 4):1649-1656
references.pmid: 16014496
references.citation: Appl Environ Microbiol. 2012 Sep;78(17):6285-94
references.pmid: 22752174
references.citation: Infect Control Hosp Epidemiol. 2004 Dec;25(12):1042-9
references.pmid: 15636290
references.citation: J Clin Microbiol. 2004 Jul;42(7):3000-11
references.pmid: 15243051
references.citation: Res Microbiol. 2007 May;158(4):393-7
references.pmid: 17398074
references.citation: Proc Natl Acad Sci U S A. 2005 Mar 29;102(13):4860-5
references.pmid: 15769858
references.citation: J Infect. 1995 Jul;31(1):39-44
references.pmid: 8522830
references.citation: Lancet. 1994 May 7;343(8906):1137-41
references.pmid: 7910236
references.citation: Tuber Lung Dis. 1992 Jun;73(3):141-4
references.pmid: 1421346
references.citation: Appl Environ Microbiol. 2008 Aug;74(15):4966-8
references.pmid: 18539815
references.citation: Appl Environ Microbiol. 2006 Jan;72(1):378-83
references.pmid: 16391067
references.citation: J Clin Microbiol. 1993 Feb;31(2):175-8
references.pmid: 8381805
references.citation: Scand J Infect Dis. 1999;31(1):63-8
references.pmid: 10381220
references.citation: Appl Environ Microbiol. 2004 Dec;70(12):7571-3
references.pmid: 15574964
references.citation: Appl Environ Microbiol. 2009 Jul;75(14):4736-46
references.pmid: 19465529
references.citation: Appl Environ Microbiol. 2011 Jan;77(2):634-41
references.pmid: 21097586
references.citation: Appl Environ Microbiol. 2013 Feb;79(3):825-34
references.pmid: 23160134