title: Mechanisms involved in the antiproliferative and proapoptotic effects of unsaponifiable fraction of extra virgin olive oil on HT-29 cancer cells.
abstract: Extra virgin olive oil (EVOO) has demonstrated great oncostatic and antiinflammatory properties. Nowadays, it is clear that unsaponifiable fraction (UF) as well as other minor EVOO components have a key role in these beneficial effects. The present study was designed to evaluate UF effect in HT-29 human colon adenocarcinoma cells. Cell growth and viability assays were determined by sulphorhodamine B test at different time points (24, 48, and 72 h). The proapoptotic effect was evaluated by flow cytometric studies and different protein expression were determined by immunoblotting. UF μg/mL concentrations' range significantly reduced the growth of HT-29 cell line. Moreover UF induced intrinsic apoptotic pathway in HT-29 human colon adenocarcinoma cells through PPARγ and NFκB signaling pathways coming up to COX-2 downregulation and modulating p53 suppressor protein levels. The results suggest that UF of EVOO may exert an important role in the anticancer effect of EVOO and provide a natural resource for the prevention or treatment of human colon cancer.
authors.affiliation: Department of Pharmacology, Faculty of Pharmacy, University of Seville, Seville, Spain.
authors.forename: Ana
authors.initials: A
authors.lastname: Cárdeno
authors.forename: Marina
authors.initials: M
authors.lastname: Sánchez-Hidalgo
authors.forename: Amparo
authors.initials: A
authors.lastname: Cortes-Delgado
authors.forename: Catalina
authors.initials: C
authors.lastname: Alarcón de la Lastra
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D051546:Cyclooxygenase 2 / Q000235:genetics / Q000378:metabolism; D015536:Down-Regulation; D019073:HT29 Cells; D006801:Humans; D016328:NF-kappa B / Q000235:genetics / Q000378:metabolism; D000069463:Olive Oil; D047495:PPAR gamma / Q000235:genetics / Q000378:metabolism; D010938:Plant Oils / Q000494:pharmacology*; D059808:Polyphenols / Q000008:administration & dosage; D012235:Rhodamines / Q000378:metabolism; D015398:Signal Transduction; D016159:Tumor Suppressor Protein p53 / Q000235:genetics / Q000378:metabolism
chemical_list: D000970:Antineoplastic Agents; D016328:NF-kappa B; D000069463:Olive Oil; D047495:PPAR gamma; D010938:Plant Oils; D059808:Polyphenols; D012235:Rhodamines; C495901:TP53 protein, human; D016159:Tumor Suppressor Protein p53; C022027:lissamine rhodamine B; D051546:Cyclooxygenase 2; C496540:PTGS2 protein, human