title: The stimulatory effect of ROCK inhibitor on bovine corneal endothelial cells.
abstract: Reagents which can promote the proliferation, adhesion and migration of cultured corneal endothelial cells (CECs) will be helpful for the treatment of reduced visual acuity due to CECs deficiency. The objectives of this study were to investigate the potential use of an inhibitor of Rho-associated protein kinase (ROCK), Y-27632, to cultured bovine corneal endothelial cells (B-CECs) and evaluated its effects on the proliferation, adhesion and migration of B-CECs. The proliferation of cultured B-CECs was moderately enhanced by 10 μM Y-27632. Y-27632 induced fibroblast-like morphological changes in the cultured B-CECs and normal cell morphology could recover after Y-27632 removal. In addition, Y-27632 was found to significantly enhance the adhesion and migration of B-CECs. Furthermore, the hanging drop aggregation assay showed that Y-27632 promoted B-CECs to form cellular networks and sheets, which proliferated along the liquid-air interface and migrated to the surface of the lid of dish. Our study demonstrated that Y-27632 is a potentially powerful reagent which can enhance the proliferation of cultured B-CECs. Y-27632 will be useful in CEC injection therapy and topical application for CEC deficiency.
authors.affiliation: Key Laboratory for Regenerative Medicine, Ministry of Education, Jinan University, Guangzhou, China.
authors.forename: Shanyi
authors.initials: S
authors.lastname: Li
authors.forename: Chan
authors.initials: C
authors.lastname: Wang
authors.forename: Ying
authors.initials: Y
authors.lastname: Dai
authors.forename: Yan
authors.initials: Y
authors.lastname: Yang
authors.forename: Hongwei
authors.initials: H
authors.lastname: Pan
authors.forename: Jingxiang
authors.initials: J
authors.lastname: Zhong
authors.forename: Jiansu
authors.initials: J
authors.lastname: Chen
mesh_terms: D000577:Amides / Q000494:pharmacology*; D000818:Animals; D002417:Cattle; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000166:cytology / Q000187:drug effects*; D004728:Endothelium, Corneal / Q000166:cytology / Q000187:drug effects*; D011725:Pyridines / Q000494:pharmacology*; D054460:rho-Associated Kinases / Q000037:antagonists & inhibitors*
chemical_list: D000577:Amides; D011725:Pyridines; C108830:Y 27632; D054460:rho-Associated Kinases
keywords: Adhesion; Cell therapy; Corneal endothelial cells; Migration; Proliferation; Y-27632