title: Purification and characterization of antioxidant components from the fruiting bodies of Pleurotus abalonus including 9-beta-d-ribofuranosidoadenine, 5'-deoxy-5'-(methylthio)adenosine, and a triterpenoid.
abstract: Although Pleurotus abalonus is a well-known edible mushroom in Asia, there is a dearth of information on its antioxidant activity. The present report is the first one focused on the purification and characterization of 9-beta-d-ribofuranosidoadenine (ADO), 5'-deoxy-5'-(methylthio) adenosine (MTA) and a triterpenoid complex from P. abalonus. Different antioxidant activities including inhibitory effects on hemolysis and lipid peroxidation in brain and kidney homogenates as well as significant synergistic effect on scavenging of hydroxyl radicals were demonstrated, which lays a foundation for the development of P. abalonus as a natural antioxidant applied in medicine.
authors.affiliation: Department of Microbiology, Nankai University, Tianjin, China.
authors.forename: Ye Ni
authors.initials: YN
authors.lastname: Zhang
authors.affiliation: Department of Microbiology, Nankai University, Tianjin, China.
authors.forename: Min
authors.initials: M
authors.lastname: Song
authors.affiliation: School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China. Electronic address: b021770@mailserv.cuhk.edu.hk.
authors.forename: Tzi Bun
authors.initials: TB
authors.lastname: Ng
authors.affiliation: Tianjin Hospital, Tianjin, China.
authors.forename: Li
authors.initials: L
authors.lastname: Zhao
authors.affiliation: Department of Microbiology, Nankai University, Tianjin, China. Electronic address: liufang312@nankai.edu.cn.
authors.forename: Fang
authors.initials: F
authors.lastname: Liu
mesh_terms: D000241:Adenosine / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D001786:Blood Glucose / Q000187:drug effects / Q000378:metabolism; D001921:Brain / Q000187:drug effects / Q000378:metabolism; D004249:DNA Damage / Q000187:drug effects; D003839:Deoxyadenosines / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D048690:Fruiting Bodies, Fungal / Q000737:chemistry; D006461:Hemolysis / Q000187:drug effects; D017665:Hydroxyl Radical / Q000737:chemistry; D007668:Kidney / Q000187:drug effects / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D051379:Mice; D020076:Pleurotus / Q000737:chemistry*; D013872:Thionucleosides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D013997:Time Factors; D014315:Triterpenes / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology
chemical_list: D000975:Antioxidants; D001786:Blood Glucose; D003839:Deoxyadenosines; D013872:Thionucleosides; D014315:Triterpenes; D017665:Hydroxyl Radical; C008500:5'-methylthioadenosine; D000241:Adenosine
keywords: 2,2′-azobis (2-amidinopropane) dihydrochloride; 2-tert-butyl-4-methoxyphenol; 5′-Deoxy-5′-(methylthio) adenosine; 5′-deoxy-5′-(methylthio)adenosine; 9-Beta-d-ribofuranosidoadenine; 9-beta-d-ribofuranosidoadenine; AAPH; ADO; Antioxidant; BHA; IR; LC–MS; LPO; MTA; NC; PBS; PC; Pleurotus abalonus; TBA; TC; TCA; Triterpenoid; infrared spectroscopy; lipid peroxidation; liquid chromatography–mass spectrometry; nucleotide complex; phosphate buffered saline; polysaccharide–peptide; thiobarbituric acid; trichloroacetic acid; triterpenoid complex