title: OsbZIP71, a bZIP transcription factor, confers salinity and drought tolerance in rice.
abstract: The bZIP transcription factor (TF) family plays an important role in the abscisic acid (ABA) signaling pathway of abiotic stress in plants. We here report the cloning and characterization of OsbZIP71, which encodes a rice bZIP TF. Functional analysis showed that OsbZIP71 is a nuclear-localized protein that specifically binds to the G-box motif, but has no transcriptional activity both in yeast and rice protoplasts. In yeast two-hybrid assays, OsbZIP71 can form both homodimers and heterodimers with Group C members of the bZIP gene family. Expression of OsbZIP71 was strongly induced by drought, polyethylene glycol (PEG), and ABA treatments, but repressed by salt treatment. OsbZIP71 overexpressing (p35S::OsbZIP71) rice significantly improved tolerance to drought, salt and PEG osmotic stresses. In contrast, RNAi knockdown transgenic lines were much more sensitive to salt, PEG osmotic stresses, and also ABA treatment. Inducible expression (RD29A::OsbZIP71) lines were significantly improved their tolerance to PEG osmotic stresses, but hypersensitivity to salt, and insensitivity to ABA. Real-time PCR analysis revealed that the abiotic stress-related genes, OsVHA-B, OsNHX1, COR413-TM1, and OsMyb4, were up-regulated in overexpressing lines, while these same genes were down-regulated in RNAi lines. Chromatin immunoprecipitation analysis confirmed that OsbZIP71 directly binds the promoters of OsNHX1 and COR413-TM1 in vivo. These results suggest that OsbZIP71 may play an important role in ABA-mediated drought and salt tolerance in rice.
authors.affiliation: State Key Laboratory of Plant Genomics and National Center for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, China.
authors.forename: Citao
authors.initials: C
authors.lastname: Liu
authors.forename: Bigang
authors.initials: B
authors.lastname: Mao
authors.forename: Shujun
authors.initials: S
authors.lastname: Ou
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Linchuan
authors.initials: L
authors.lastname: Liu
authors.forename: Yanbin
authors.initials: Y
authors.lastname: Wu
authors.forename: Chengcai
authors.initials: C
authors.lastname: Chu
authors.forename: Xiping
authors.initials: X
authors.lastname: Wang
mesh_terms: D000040:Abscisic Acid; D055864:Droughts; D018525:Germination; D012275:Oryza / Q000235:genetics / Q000378:metabolism*; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified; D011485:Protein Binding; D021381:Protein Transport; D054712:Salinity; D012639:Seeds / Q000235:genetics / Q000502:physiology; D013312:Stress, Physiological; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D014867:Water / Q000378:metabolism*
chemical_list: D010940:Plant Proteins; D014157:Transcription Factors; D014867:Water; D000040:Abscisic Acid
references.citation: New Phytol. 2011 Jan;189(1):54-81
references.pmid: 21118256
references.citation: Plant J. 2008 Mar;53(6):935-49
references.pmid: 18088315
references.citation: Mol Plant. 2010 Mar;3(2):361-73
references.pmid: 20080816
references.citation: Plant J. 2010 Aug;63(3):417-29
references.pmid: 20487379
references.citation: Plant Cell Physiol. 2005 Oct;46(10):1623-34
references.pmid: 16051676
references.citation: Trends Plant Sci. 2002 Mar;7(3):106-11
references.pmid: 11906833
references.citation: Proc Natl Acad Sci U S A. 1999 Dec 21;96(26):15348-53
references.pmid: 10611387
references.citation: J Plant Physiol. 2007 Aug;164(8):969-79
references.pmid: 16919842
references.citation: Plant Cell. 2009 Jun;21(6):1747-61
references.pmid: 19531597
references.citation: Planta. 2011 Nov;234(5):1007-18
references.pmid: 21698458
references.citation: Anal Biochem. 2011 May 15;412(2):183-8
references.pmid: 21284925
references.citation: Curr Protein Pept Sci. 2009 Jun;10(3):244-69
references.pmid: 19519454
references.citation: Plant Sci. 2012 Sep;193-194:8-17
references.pmid: 22794914
references.citation: J Biol Chem. 2000 Jan 21;275(3):1723-30
references.pmid: 10636868
references.citation: Plant Physiol. 1999 Jan;119(1):205-12
references.pmid: 9880362
references.citation: Plant Physiol. 2008 Dec;148(4):1938-52
references.pmid: 18931143
references.citation: Planta. 2012 Jun;235(6):1157-69
references.pmid: 22189955
references.citation: Plant Physiol. 2012 Apr;158(4):1755-68
references.pmid: 22301130
references.citation: Plant Cell Physiol. 2004 Mar;45(3):346-50
references.pmid: 15047884
references.citation: Planta. 2009 Feb;229(3):605-15
references.pmid: 19048288
references.citation: FEBS Lett. 2006 Dec 11;580(28-29):6537-42
references.pmid: 17112521
references.citation: PLoS One. 2008 Aug 13;3(8):e2944
references.pmid: 18698409
references.citation: Biotechnol Lett. 2009 Nov;31(11):1811-5
references.pmid: 19618274
references.citation: Plant Cell. 2002;14 Suppl:S165-83
references.pmid: 12045276
references.citation: Mol Gen Genet. 1993 Jul;240(1):1-8
references.pmid: 8341255
references.citation: Plant Cell Environ. 2010 Dec;33(12):2209-30
references.pmid: 20807373
references.citation: Plant Physiol. 2008 Feb;146(2):333-50
references.pmid: 18065552
references.citation: Plant Mol Biol. 2009 Jan;69(1-2):107-19
references.pmid: 18841482
references.citation: Plant Cell. 2000 Mar;12(3):393-404
references.pmid: 10715325
references.citation: Nat Genet. 2011 Jun 05;43(7):715-9
references.pmid: 21642989
references.citation: Plant Mol Biol. 2006 Jan;60(1):51-68
references.pmid: 16463099
references.citation: Biochim Biophys Acta. 2000 May 1;1465(1-2):17-36
references.pmid: 10748245
references.citation: Plant Mol Biol. 2009 May;70(1-2):219-29
references.pmid: 19229638
references.citation: Plant Physiol Biochem. 2013 Jan;62:88-94
references.pmid: 23202047
references.citation: Plant Cell Rep. 2011 Aug;30(8):1383-91
references.pmid: 21476089
references.citation: Proc Natl Acad Sci U S A. 2000 Oct 10;97(21):11632-7
references.pmid: 11005831
references.citation: Plant Cell Physiol. 2006 Jan;47(1):141-53
references.pmid: 16284406
references.citation: Planta. 2011 Jan;233(1):175-88
references.pmid: 20963607
references.citation: Plant Physiol. 2003 May;132(1):64-74
references.pmid: 12746512
references.citation: Plant Cell Environ. 2002 Feb;25(2):131-139
references.pmid: 11841658
references.citation: J Exp Bot. 2001 Oct;52(363):1969-80
references.pmid: 11559732
references.citation: Annu Rev Plant Biol. 2002;53:247-73
references.pmid: 12221975
references.citation: Science. 1998 Apr 3;280(5360):104-6
references.pmid: 9525853
references.citation: Nucleic Acids Res. 2001 May 1;29(9):e45
references.pmid: 11328886
references.citation: Plant Cell Rep. 2008 Oct;27(10):1677-86
references.pmid: 18679687
references.citation: Planta. 2011 Jul;234(1):97-107
references.pmid: 21374086
references.citation: Plant J. 2010 Mar;61(6):1041-52
references.pmid: 20409277
references.citation: Plant Cell. 1994 Feb;6(2):251-64
references.pmid: 8148648
references.citation: Plant Mol Biol. 2008 Aug;67(6):589-602
references.pmid: 18470484
references.citation: Plant Mol Biol. 2008 Apr;66(6):675-83
references.pmid: 18236009
references.citation: Plant Cell Physiol. 2004 Mar;45(3):309-17
references.pmid: 15047879
references.citation: Plant Mol Biol. 2010 Mar;72(4-5):557-66
references.pmid: 20039193
references.citation: Plant Cell. 2002 Feb;14(2):343-57
references.pmid: 11884679
references.citation: Plant J. 2006 Jun;46(5):890-900
references.pmid: 16709202
references.citation: Plant Cell. 2001 Aug;13(8):1959-68
references.pmid: 11487705
references.citation: Plant J. 2003 Apr;34(2):137-48
references.pmid: 12694590
references.citation: Plant J. 2000 Apr;22(1):29-38
references.pmid: 10792818
references.citation: Plant Methods. 2006 Jun 29;2:13
references.pmid: 16808845
references.citation: Nucleic Acids Res. 1997 Dec 15;25(24):4876-82
references.pmid: 9396791
references.citation: Nat Biotechnol. 1999 Mar;17(3):287-91
references.pmid: 10096298
references.citation: Plant J. 2013 Aug;75(3):403-16
references.pmid: 23581288