title: Reconstitution of an active arginine deiminase pathway in Mycoplasma pneumoniae M129.
abstract: Some species of the genus Mycoplasma code for the arginine deiminase pathway (ADI), which enables these bacteria to produce ATP from arginine by the successive reaction of three enzymes: arginine deiminase (ArcA), ornithine carbamoyltransferase (ArcB), and carbamate kinase (ArcC). It so far appears that independently isolated strains of Mycoplasma pneumoniae encode an almost identical truncated version of the ADI pathway in which the proteins ArcA and ArcB have lost their original enzymatic activities due to the deletion of significant regions of these proteins. To study the consequences of a functional ADI pathway, M. pneumoniae M129 was successfully transformed with the cloned functional arcA, arcB, and arcC genes from Mycoplasma fermentans. Enzymatic tests showed that while the M. pneumoniae ArcAB and ArcABC transformants possess functional arginine deiminase, ornithine carbamoyltransferase, and carbamate kinase, they were unable to grow on arginine as the sole energy source. Nevertheless, infection of a lung epithelial cell line, A549, with the M. pneumoniae transformants showed that almost 100% of the infected host cells were nonviable, while most of the lung cells infected with nontransformed M. pneumoniae were viable under the same experimental conditions.
authors.affiliation: Department of Microbiology and Molecular Genetics, The Hebrew University-Hadassah Medical School, Jerusalem, Israel.
authors.forename: Hagai
authors.initials: H
authors.lastname: Rechnitzer
authors.forename: Shlomo
authors.initials: S
authors.lastname: Rottem
authors.forename: Richard
authors.initials: R
authors.lastname: Herrmann
mesh_terms: D000595:Amino Acid Sequence; D045744:Cell Line, Tumor; D003001:Cloning, Molecular; D004847:Epithelial Cells / Q000382:microbiology; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology*; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology*; D006801:Humans; D006867:Hydrolases / Q000235:genetics / Q000378:metabolism*; D009177:Mycoplasma pneumoniae / Q000201:enzymology* / Q000378:metabolism; D020545:Respiratory Mucosa / Q000166:cytology
chemical_list: D006867:Hydrolases; C019723:arginine deiminase
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