title: Downregulated expression of the cyclase-associated protein 1 (CAP1) reduces migration in esophageal squamous cell carcinoma.
abstract: OBJECTIVE
  Overexpression of cyclase-associated proteins has been associated with poor prognosis in several human cancers. Cyclase-associated protein 1 is a member of the cyclase-associated proteins which contributes to tumor progression. The aim of the present study was to examine the expression of cyclase-associated protein 1 and to elucidate its clinicopathologic signiﬁcance in a larger series of esophageal squamous cell carcinoma.
  
  
  METHODS
  Immunohistochemical and western blot analyses were performed in esophageal squamous cell carcinoma tissues. Survival analyses were performed by using the Kaplan-Meier method. The role of cyclase-associated protein 1 in migration was studied in esophageal squamous cell carcinoma cell lines of TE1 through knocking down cyclase-associated protein 1 with siRNA and overexpression of cyclase-associated protein 1. The regulation of cyclase-associated protein 1 on migration was determined by transwell and wound-healing assays.
  
  
  RESULTS
  Immunohistochemical analysis showed that cyclase-associated protein 1 expression was negatively associated with E-cadherin and signiﬁcantly associated with lymph node metastases. Survival analysis revealed that cyclase-associated protein 1 overexpression was signiﬁcantly associated with overall survival (P = 0.011). Knock down of cyclase-associated protein 1 in TE1 cells resulted in decreased vimentin and F-actin levels and the capability for migration. In addition, overexpression of cyclase-associated protein 1 promoted the migration of TE1 cells.
  
  
  CONCLUSIONS
  These ﬁndings suggest that cyclase-associated protein 1 is involved in the metastasis of esophageal squamous cell carcinoma, and that elevated levels of cyclase-associated protein 1 expression may indicate a poor prognosis for patients with esophageal squamous cell carcinoma.
authors.affiliation: Department of Pathology, Afﬁliated Hospital of Nantong University, Jiangsu 226001, PR China.
authors.forename: Mei
authors.initials: M
authors.lastname: Li
authors.forename: Xiaojing
authors.initials: X
authors.lastname: Yang
authors.forename: Hui
authors.initials: H
authors.lastname: Shi
authors.forename: Hanru
authors.initials: H
authors.lastname: Ren
authors.forename: Xueyu
authors.initials: X
authors.lastname: Chen
authors.forename: Shu
authors.initials: S
authors.lastname: Zhang
authors.forename: Junya
authors.initials: J
authors.lastname: Zhu
authors.forename: Jianguo
authors.initials: J
authors.lastname: Zhang
mesh_terms: D000199:Actins / Q000378:metabolism; D000368:Aged; D015153:Blotting, Western; D015820:Cadherins / Q000378:metabolism; D002294:Carcinoma, Squamous Cell / Q000378:metabolism*; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D003598:Cytoskeletal Proteins / Q000235:genetics / Q000378:metabolism*; D015536:Down-Regulation; D004938:Esophageal Neoplasms / Q000378:metabolism*; D000077277:Esophageal Squamous Cell Carcinoma; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D055785:Gene Knockdown Techniques; D006801:Humans; D007150:Immunohistochemistry; D053208:Kaplan-Meier Estimate; D008207:Lymphatic Metastasis; D008297:Male; D034741:RNA, Small Interfering / Q000378:metabolism
chemical_list: D000199:Actins; C489549:CAP1 protein, human; D015820:Cadherins; D018797:Cell Cycle Proteins; D003598:Cytoskeletal Proteins; D034741:RNA, Small Interfering
keywords: carcinoma; cell migration; squamous cell