title: Pneumococcal polysaccharide vaccination induces polysaccharide-specific B cells in adult peripheral blood expressing CD19⁺CD20⁺CD3⁻CD70⁻CD27⁺IgM⁺CD43⁺CD5⁺/⁻.
abstract: Pneumococcal polysaccharide vaccines have been used to elicit a protective anti-pneumococcal polysaccharide antibody response against Streptococcus pneumoniae in healthy individuals. Identifying human B cells which respond to T-cell independent type-2 antigens, such as pneumococcal polysaccharides, has been challenging. We employed pneumococcal polysaccharides directly conjugated to fluorophores in conjunction with flow cytometry to identify the phenotype of B cells that respond to pneumococcal polysaccharide vaccination. We have previously identified that the majority of pneumococcal polysaccharide-selected cells responding to vaccination are CD27(+)IgM(+) (IgM(+) memory) cells. In this study, we further characterized pneumococcal polysaccharide-selected cells in the peripheral blood to better identify how the various B cell phenotypes responded 7 and 30 days post-immunization. We show that 7 days post-immunization the majority of pneumococcal polysaccharide-selected IgM(+) memory cells (PPS14(+) 56.5%, PPS23F(+) 63.8%) were CD19(+)CD20(+)CD27(+)IgM(+)CD43(+)CD5(+/-)CD70(-), which was significantly increased compared to pre-immunization levels. This phenotype is in alignment with recent publications describing human B-1 cells. PPS-responsive B cells receded to pre-immunization levels by day-30. These findings suggest that this B-1 like cell population plays an important role in early responses to S. pneumoniae infection and possibly other T-cell independent type-2 antigens in humans.
authors.affiliation: Department of Medicine, University of Toledo, Toledo, OH 43614, United States.
authors.forename: David J
authors.initials: DJ
authors.lastname: Leggat
authors.forename: Noor M
authors.initials: NM
authors.lastname: Khaskhely
authors.forename: Anita S
authors.initials: AS
authors.lastname: Iyer
authors.forename: Jason
authors.initials: J
authors.lastname: Mosakowski
authors.forename: Rebecca S
authors.initials: RS
authors.lastname: Thompson
authors.forename: John D
authors.initials: JD
authors.lastname: Weinandy
authors.forename: M A Julie
authors.initials: MA
authors.lastname: Westerink
mesh_terms: D000328:Adult; D015703:Antigens, CD / Q000032:analysis*; D001402:B-Lymphocytes / Q000737:chemistry* / Q000276:immunology*; D005260:Female; D006801:Humans; D007075:Immunoglobulin M / Q000032:analysis*; D016130:Immunophenotyping; D008297:Male; D022242:Pneumococcal Vaccines / Q000008:administration & dosage / Q000276:immunology*; D011135:Polysaccharides, Bacterial / Q000276:immunology*; D013296:Streptococcus pneumoniae / Q000276:immunology; D013997:Time Factors; D055815:Young Adult
chemical_list: D015703:Antigens, CD; D007075:Immunoglobulin M; D022242:Pneumococcal Vaccines; D011135:Polysaccharides, Bacterial
keywords: Ab; B cell; CD43; FMO; Flow cytometry; Fluorescence Minus One; MZB; OPT; PPS; PPV; Polysaccharide; Streptococcus pneumoniae; T-independent type II; TI-2; antibodies; opsonophagocytic titer; pneumococcal polysaccharide; pneumococcal polysaccharide vaccination; pneumococcus; splenic marginal zone B cell
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