title: Protective effect of mitochondria-targeted antioxidants in an acute bacterial infection.
abstract: Acute pyelonephritis is a potentially life-threatening infection of the upper urinary tract. Inflammatory response and the accompanying oxidative stress can contribute to kidney tissue damage, resulting in infection-induced intoxication that can become fatal in the absence of antibiotic therapy. Here, we show that pyelonephritis was associated with oxidative stress and renal cell death. Oxidative stress observed in pyelonephritic kidney was accompanied by a reduced level of mitochondrial B-cell lymphoma 2 (Bcl-2). Importantly, renal cell death and animal mortality were both alleviated by mitochondria-targeted antioxidant 10(6'-plastoquinonyl) decylrhodamine 19 (SkQR1). These findings suggest that pyelonephritis can be treated by reducing mitochondrial reactive oxygen species and thus by protecting mitochondrial integrity and lowering kidney damage.
authors.affiliation: A. N. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow 119992, Russia.
authors.forename: Egor Y
authors.initials: EY
authors.lastname: Plotnikov
authors.forename: Maria A
authors.initials: MA
authors.lastname: Morosanova
authors.forename: Irina B
authors.initials: IB
authors.lastname: Pevzner
authors.forename: Ljubava D
authors.initials: LD
authors.lastname: Zorova
authors.forename: Vasily N
authors.initials: VN
authors.lastname: Manskikh
authors.forename: Natalya V
authors.initials: NV
authors.lastname: Pulkova
authors.forename: Svetlana I
authors.initials: SI
authors.lastname: Galkina
authors.forename: Vladimir P
authors.initials: VP
authors.lastname: Skulachev
authors.forename: Dmitry B
authors.initials: DB
authors.lastname: Zorov
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology* / Q000627:therapeutic use; D015153:Blotting, Western; D002478:Cells, Cultured; D016503:Drug Delivery Systems / Q000379:methods; D004926:Escherichia coli; D007150:Immunohistochemistry; D008855:Microscopy, Electron, Scanning; D008928:Mitochondria / Q000187:drug effects* / Q000378:metabolism; D018384:Oxidative Stress / Q000502:physiology*; D009195:Peroxidase / Q000378:metabolism; D010971:Plastoquinone / Q000031:analogs & derivatives* / Q000494:pharmacology / Q000627:therapeutic use; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism*; D011704:Pyelonephritis / Q000188:drug therapy* / Q000473:pathology; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism*; D012235:Rhodamines / Q000494:pharmacology* / Q000627:therapeutic use
chemical_list: C534903:10-(6'-plastoquinonyl) decylrhodamine 19; D000975:Antioxidants; D019253:Proto-Oncogene Proteins c-bcl-2; D017382:Reactive Oxygen Species; D012235:Rhodamines; D009195:Peroxidase; D010971:Plastoquinone
keywords: inflammation; innate immunity; phenoptosis; toll-like receptors; urological diseases
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