title: [Assessment of the capability of the fluid model of automatic blood cell analyzer in white blood cell count and classification].
abstract: OBJECTIVE
  To evaluate the application of XT-4000i automatic blood cell analyzer in white cell count and classification of cerebrospinal fluid (CSF).
  
  
  METHODS
  The fluid model of XT-4000i automatic blood cell analyzer was directly used to detect the white cell count and classification in 200 samples of CSF, and compared with the results obtained by manually microscopic counting method. White blood cell classification was performed by manual method under microscope with Wright's staining,and instrumental method with fluorescence staining and flow cytometry.
  
  
  RESULTS
  There is no statistical difference between instrumental and manual method in detecting the absolute counting of WBC, RBC, mononuclear cell and multinucleate cells (P>0.05), and there is a good correlation between the two methods (r=0.987, 0.999, 0.981 and 0.983 for WBC, RBC, mononuclear cell and multinucleate cell counts). Tumor cells in the samples with high fluorescent staining by instrumental method can be identified with Wright's staining by microscope method, which were consistent with the clinical diagnosis.
  
  
  CONCLUSIONS
  The fluid model of XT-4000i automatic blood cell analyzer was rapid and accurate in CSF white cell count and classification,and it also can provide preliminary information for tumor cells screening. The fluid model of XT-4000i automatic blood cell analyzer in white cell count and classification of CSF has the value of popularization in clinical application.
authors.affiliation: Department of Clinical Laboratory, China-Japan Friendship Hospital, Beijing 100029, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Shen
authors.forename: Yin
authors.initials: Y
authors.lastname: Chen
authors.forename: Guo-Zhen
authors.initials: GZ
authors.lastname: Wang
authors.forename: Liang
authors.initials: L
authors.lastname: Ma
authors.forename: Cheng-Wu
authors.initials: CW
authors.lastname: Han
mesh_terms: D001322:Autoanalysis / Q000295:instrumentation / Q000379:methods; D002555:Cerebrospinal Fluid / Q000166:cytology; D006801:Humans; D007958:Leukocyte Count / Q000295:instrumentation* / Q000379:methods*; D007962:Leukocytes / Q000145:classification*