title: Seroreactivity to Plasmodium falciparum erythrocyte membrane protein 1 intracellular domain in malaria-exposed children and adults.
abstract: Plasmodium falciparum erythrocyte membrane protein 1 (PfEMP1) antigens mediate parasite sequestration and host immune evasion. Reactivity to 21 PfEMP1 fragments on a protein microarray was measured in serum samples from Malian children aged 1-6 years and adults. Seroreactivity to PfEMP1 fragments was higher in adults than in children; intracellular conserved fragments were more widely recognized than were extracellular hypervariable fragments. Over a malaria season, children maintained this differential seroreactivity and recognized additional intracellular PfEMP1 fragments. This approach has the potential to identify conserved, seroreactive extracellular PfEMP1 domains critical for protective immunity to malaria.
authors.affiliation: Howard Hughes Medical Institute/Center for Vaccine Development, University of Maryland School of Medicine, Baltimore.
authors.forename: Mark A
authors.initials: MA
authors.lastname: Travassos
authors.forename: Amadou
authors.initials: A
authors.lastname: Niangaly
authors.forename: Jason A
authors.initials: JA
authors.lastname: Bailey
authors.forename: Amed
authors.initials: A
authors.lastname: Ouattara
authors.forename: Drissa
authors.initials: D
authors.lastname: Coulibaly
authors.forename: Matthew B
authors.initials: MB
authors.lastname: Laurens
authors.forename: Jozelyn
authors.initials: J
authors.lastname: Pablo
authors.forename: Algis
authors.initials: A
authors.lastname: Jasinskas
authors.forename: Rie
authors.initials: R
authors.lastname: Nakajima-Sasaki
authors.forename: Andrea A
authors.initials: AA
authors.lastname: Berry
authors.forename: Shannon
authors.initials: S
authors.lastname: Takala-Harrison
authors.forename: Bourema
authors.initials: B
authors.lastname: Kouriba
authors.forename: J Alexandra
authors.initials: JA
authors.lastname: Rowe
authors.forename: Kirsten E
authors.initials: KE
authors.lastname: Lyke
authors.forename: Ogobara K
authors.initials: OK
authors.lastname: Doumbo
authors.forename: Mahamadou A
authors.initials: MA
authors.lastname: Thera
authors.forename: Philip L
authors.initials: PL
authors.lastname: Felgner
authors.forename: Christopher V
authors.initials: CV
authors.lastname: Plowe
mesh_terms: D000328:Adult; D000913:Antibodies, Protozoan / Q000097:blood; D000953:Antigens, Protozoan / Q000276:immunology*; D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D006801:Humans; D007223:Infant; D016778:Malaria, Falciparum / Q000097:blood / Q000276:immunology*; D010446:Peptide Fragments / Q000276:immunology*; D010963:Plasmodium falciparum / Q000276:immunology; D040081:Protein Array Analysis; D017434:Protein Structure, Tertiary; D015800:Protozoan Proteins / Q000276:immunology*
chemical_list: D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D010446:Peptide Fragments; D015800:Protozoan Proteins; C085731:erythrocyte membrane protein 1, Plasmodium falciparum
keywords: PfEMP1; immunity; malaria; microarray; seroreactivity; var genes
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