title: Molecular typing of haemorrhagic septicaemia-associated Pasteurella multocida isolates from Pakistan and Thailand using multilocus sequence typing and pulsed-field gel electrophoresis.
abstract: A comparative genetic study of 23 field isolates and vaccine strains of Pasteurella multocida associated with haemorrhagic septicaemia cases from Pakistan and Thailand was done using pulsed field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). The MLST sequence type (ST) for all 20 of the 23 isolates tested was 122. The PFGE results showed one band difference between the Pakistani and the Thai isolates. Sequence type 122 is the dominant associated profile with haemorrhagic septicaemia (HS) cases in South Asia. The study supports the concept of using PFGE for short-term epidemiology and MLST for long-term epidemiology.
authors.affiliation: School of Veterinary and Life Sciences, Murdoch University, South Street, Murdoch, Western Australia 6150, Australia.
authors.forename: Ahmed M
authors.initials: AM
authors.lastname: Moustafa
authors.forename: Mark D
authors.initials: MD
authors.lastname: Bennett
authors.forename: John
authors.initials: J
authors.lastname: Edwards
authors.forename: Kamran
authors.initials: K
authors.lastname: Azim
authors.forename: Muhammed A
authors.initials: MA
authors.lastname: Mesaik
authors.forename: M Iqbal
authors.initials: MI
authors.lastname: Choudhary
authors.forename: Pornpen
authors.initials: P
authors.lastname: Pathanasophon
authors.forename: Apasara
authors.initials: A
authors.lastname: Worarach
authors.forename: Qurban
authors.initials: Q
authors.lastname: Ali
authors.forename: Muhammad
authors.initials: M
authors.lastname: Abubakar
authors.forename: Rehana
authors.initials: R
authors.lastname: Anjum
mesh_terms: D000818:Animals; D015373:Bacterial Typing Techniques / Q000662:veterinary; D002020:Buffaloes / Q000382:microbiology; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology / Q000382:microbiology; D016521:Electrophoresis, Gel, Pulsed-Field / Q000662:veterinary*; D006483:Hemorrhagic Septicemia / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D058885:Multilocus Sequence Typing / Q000662:veterinary*; D060885:Multiplex Polymerase Chain Reaction / Q000662:veterinary; D010154:Pakistan / Q000453:epidemiology; D016979:Pasteurella multocida / Q000145:classification*; D013785:Thailand / Q000453:epidemiology
keywords: Haemorrhagic septicaemia; Multilocus sequence typing; Pakistan; Pasteurella multocida; Pulsed field gel electrophoresis; Thailand