title: IL-36 cytokine expression and its relationship with p38 MAPK and NF-κB pathways in psoriasis vulgaris skin lesions.
abstract: This study examined the correlation of the expression of interleukin-36 (IL-36), a novel member of interleukin-1 (IL-1) family, with p38 mitogen-activated protein kinase (p38 MAPK) and nuclear factor-kappa B (NF-κB) pathways in psoriasis vulgaris skin lesions. The expression levels of IL-36α, IL-36β, IL-36Γ, phosphorylated p38 MAPK, and NF-κBp65 were detected in the skin tissues of 38 psoriasis patients and 17 healthy control subjects by real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR) and Western blotting. The cytokine expression levels were compared between the psoriasis group and the control group. A correlation analysis between cytokine proteins was performed in the psoriasis group. Results showed that the expression levels of IL-36a, IL-36β, IL-36Γ, phosphorylated p38 MAPK and NF-κBp65 in the psoriasis group were significantly higher than those in the control group (P<0.001). In the psoriasis group, the IL-36 cytokine expression was positively correlated with phosphorylated p38 MAPK and NF-κBp65 expression (P<0.05). A significant positive correlation was also found between the phosphorylated p38 MAPK and NF-κBp65 expression (P<0.01). It was concluded that the increased IL-36 expression is correlated with p38 MAPK and NF-κB pathways in psoriasis vulgaris skin lesions. All the three factors may be jointly involved in the pathogenesis and local inflammatory response of psoriasis.
authors.affiliation: Department of Dermatology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: He
authors.affiliation: Department of Dermatology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China.
authors.forename: Hong-Xiang
authors.initials: HX
authors.lastname: Chen
authors.affiliation: Department of Dermatology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China.
authors.forename: Wen
authors.initials: W
authors.lastname: Li
authors.affiliation: Department of Dermatology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Department of Dermatology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China.
authors.forename: Shan-Juan
authors.initials: SJ
authors.lastname: Chen
authors.affiliation: Department of Dermatology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Yue
authors.affiliation: Department of Dermatology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China.
authors.forename: Min
authors.initials: M
authors.lastname: Xiao
authors.affiliation: Department of Dermatology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China. catty0004@163.comxxx.
authors.forename: Jia-Wen
authors.initials: JW
authors.lastname: Li
mesh_terms: D000293:Adolescent; D000328:Adult; D016207:Cytokines / Q000235:genetics*; D005260:Female; D006801:Humans; D007375:Interleukin-1 / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D016328:NF-kappa B / Q000235:genetics*; D011565:Psoriasis / Q000235:genetics*; D015398:Signal Transduction / Q000235:genetics*; D012867:Skin / Q000473:pathology*; D055815:Young Adult; D048051:p38 Mitogen-Activated Protein Kinases / Q000235:genetics*
chemical_list: D016207:Cytokines; D007375:Interleukin-1; D016328:NF-kappa B; C572887:interleukin 36, human; D048051:p38 Mitogen-Activated Protein Kinases
references.citation: Clin Exp Allergy. 2000 Jan;30(1):48-55
references.pmid: 10606930
references.citation: Oncogene. 2006 Oct 30;25(51):6731-48
references.pmid: 17072325
references.citation: N Engl J Med. 2009 Jul 30;361(5):496-509
references.pmid: 19641206
references.citation: Eur Cell Mater. 2012 Feb 16;23:103-19; discussion 119-20
references.pmid: 22354461
references.citation: J Dermatol. 2009 Oct;36(10):534-40
references.pmid: 19785707
references.citation: Cell Signal. 2003 Jan;15(1):1-7
references.pmid: 12401514
references.citation: Eur J Biochem. 2004 Feb;271(4):785-91
references.pmid: 14764095
references.citation: J Invest Dermatol. 2007 Mar;127(3):514-25
references.pmid: 17299434
references.citation: J Biol Chem. 2002 Apr 26;277(17):14884-93
references.pmid: 11842088
references.citation: Curr Drug Targets Inflamm Allergy. 2005 Jun;4(3):329-34
references.pmid: 16101542
references.citation: Curr Opin Oncol. 2008 Mar;20(2):183-9
references.pmid: 18300768
references.citation: J Exp Med. 2007 Oct 29;204(11):2603-14
references.pmid: 17908936
references.citation: J Immunol. 2010 Oct 1;185(7):4354-62
references.pmid: 20833839
references.citation: Exp Mol Pathol. 2007 Dec;83(3):413-8
references.pmid: 17599830
references.citation: Clin Exp Dermatol. 2001 Jun;26(4):314-20
references.pmid: 11422182
references.citation: Infect Immun. 2005 Jul;73(7):3990-8
references.pmid: 15972486
references.citation: J Immunol. 2011 Feb 15;186(4):2613-22
references.pmid: 21242515
references.citation: Am J Physiol Heart Circ Physiol. 2003 Apr;284(4):H1095-103
references.pmid: 12505871
references.citation: J Biol Chem. 2004 Apr 2;279(14):13677-88
references.pmid: 14734551
references.citation: Sci STKE. 2006 Oct 17;2006(357):re13
references.pmid: 17047224
references.citation: Semin Cutan Med Surg. 2010 Mar;29(1):3-9
references.pmid: 20430301
references.citation: Nat Rev Immunol. 2010 Feb;10(2):89-102
references.pmid: 20081871
references.citation: Curr Opin Pharmacol. 2012 Aug;12(4):486-90
references.pmid: 22398321
references.citation: J Invest Dermatol. 2011 Dec;131(12):2428-37
references.pmid: 21881584
references.citation: Cell. 2008 Feb 8;132(3):344-62
references.pmid: 18267068