title: In vitro antimalarial activity of extracts of some plants from a biological reserve in Costa Rica.
abstract: Treatment with the usual antimalarial drugs, have induced parasite resistance, reinforcing the need to finding natural antimalarial components that would be found on plants from the forest. Therefore, we decided to look for these components in Costa Rican plants from a protected forest area. Fresh and dry extracts of roots, bark, leaves, flowers and fruits of 25 plants from a biological reserve in Costa Rica, Reserva Biol6gica Alberto Manuel Brenes (REBAMB), were studied in vitro for the presence of substances with antimalarial activity. By studying the inhibition of P berghei schizogony, we assessed the antimalarial activity of several plant extracts: Aphelandra aurantiaca, A. tridentata (Acanthaceae); Xanthosoma undipes (Araceae); Iriartea deltoidea (Arecaceae); Neurolaena lobata (Asteraceae); Senna papillosa, Pterocarpus hayessi, Lonchocarpus pentaphyllus (Fabaceae); Nectandra membranacea, Persea povedae, Cinamomum chavarrianum (Lauraceae); Hampea appendiculata (Malvaceae); Ruagea glabra, Guarea glabra (Meliaceae); Psidium guajava (Myrtaceae); Bocconia frutescens (Papaveraceae); Piper friedrichsthalii (Piperaceae); Clematis dioica (Ranunculaceae); Prunus annularis (Rosaceae); Siparuna thecaphora (Siparunaceae); Solanum arboreum, Witheringia solanacea (Solanaceae); Ticodendrum incognitum (Ticodendraceae); Heliocarpus appendiculatus (Tiliaceae) and Myriocarpa longipes (Urticaceae). We used different parts of the plants as well as fresh and dried extracts for testing IC50. The solid content of the extracts ranged from 1-71.9 microg/mL. The fresh extracts showed stronger activity than the dry ones. Since the plants showing the strongest antimalarial activity are very common in Central America, and some similar genera of these plants have shown positives results in South America, we considered important to present these findings for discussion. On the other hand, this is the first systematic study of this kind ever realized in a circumscribed and protected area of Costa Rica.
authors.affiliation: Laboratorio de Investigación Universidad de Ciencias Medicas "Dr. Andrds Vesalio Guzmin" (UCIMED), San JosC, Costa Rica, Amdrica Central. chinchillacm@ucimed.com
authors.forename: Misael
authors.initials: M
authors.lastname: Chinchilla
authors.forename: Idalia
authors.initials: I
authors.lastname: Valerio
authors.forename: Ronald
authors.initials: R
authors.lastname: Sánchez
authors.forename: Víctor
authors.initials: V
authors.lastname: Mora
authors.forename: Vanessa
authors.initials: V
authors.lastname: Bagnarello
authors.forename: Laura
authors.initials: L
authors.lastname: Martínez
authors.forename: Antonieta
authors.initials: A
authors.lastname: Gonzalez
authors.forename: Juan Carlos
authors.initials: JC
authors.lastname: Vanegas
authors.forename: Alvaro
authors.initials: A
authors.lastname: Apestegui
mesh_terms: D000818:Animals; D000962:Antimalarials / Q000494:pharmacology*; D005260:Female; D020128:Inhibitory Concentration 50; D019684:Magnoliopsida / Q000737:chemistry* / Q000145:classification; D008297:Male; D051379:Mice; D021261:Parasitic Sensitivity Tests; D010936:Plant Extracts / Q000494:pharmacology*; D010962:Plasmodium berghei / Q000187:drug effects*
chemical_list: D000962:Antimalarials; D010936:Plant Extracts