title: rFN/Cad-11-modified collagen type II biomimetic interface promotes the adhesion and chondrogenic differentiation of mesenchymal stem cells.
abstract: Properties of the cell-material interface are determining factors in the successful function of cells for cartilage tissue engineering. Currently, cell adhesion is commonly promoted through the use of polypeptides; however, due to their lack of complementary or modulatory domains, polypeptides must be modified to improve their ability to promote adhesion. In this study, we utilized the principle of matrix-based biomimetic modification and a recombinant protein, which spans fragments 7-10 of fibronectin module III (heterophilic motif) and extracellular domains 1-2 of cadherin-11 (rFN/Cad-11) (homophilic motif), to modify the interface of collagen type II (Col II) sponges. We showed that the designed material was able to stimulate cell proliferation and promote better chondrogenic differentiation of rabbit mesenchymal stem cells (MSCs) in vitro than both the FN modified surfaces and the negative control. Further, the Col II/rFN/Cad-11-MSCs composite stimulated cartilage formation in vivo; the chondrogenic effect of Col II alone was much less significant. These results suggested that the rFN/Cad-11-modified collagen type II biomimetic interface has dual biological functions of promoting adhesion and stimulating chondrogenic differentiation. This substance, thus, may serve as an ideal scaffold material for cartilage tissue engineering, enhancing repair of injured cartilage in vivo.
authors.affiliation: 1 National & Regional United Engineering Laboratory of Tissue Engineering, Department of Biomedical Materials Science, College of Biomedical Engineering, Third Military Medical University , Chongqing, China .
authors.forename: Shiwu
authors.initials: S
authors.lastname: Dong
authors.forename: Hongfeng
authors.initials: H
authors.lastname: Guo
authors.forename: Yuan
authors.initials: Y
authors.lastname: Zhang
authors.forename: Zhengsheng
authors.initials: Z
authors.lastname: Li
authors.forename: Fei
authors.initials: F
authors.lastname: Kang
authors.forename: Bo
authors.initials: B
authors.lastname: Yang
authors.forename: Xia
authors.initials: X
authors.lastname: Kang
authors.forename: Can
authors.initials: C
authors.lastname: Wen
authors.forename: Yanfei
authors.initials: Y
authors.lastname: Yan
authors.forename: Bo
authors.initials: B
authors.lastname: Jiang
authors.forename: Yujiang
authors.initials: Y
authors.lastname: Fan
mesh_terms: D000818:Animals; D032701:Biomimetics; D015820:Cadherins / Q000737:chemistry; D002448:Cell Adhesion / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D020219:Chondrogenesis / Q000187:drug effects; D024043:Collagen Type II / Q000737:chemistry*; D005353:Fibronectins / Q000737:chemistry; D007150:Immunohistochemistry; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects; D051379:Mice; D008819:Mice, Nude; D008855:Microscopy, Electron, Scanning; D011817:Rabbits; D060888:Real-Time Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000737:chemistry / Q000494:pharmacology
chemical_list: D015820:Cadherins; D024043:Collagen Type II; D005353:Fibronectins; D011994:Recombinant Proteins; C086960:osteoblast cadherin
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