title: Co-occurrence of five Fusarium toxins in corn-Dried Distiller's Grains with Solubles in Thailand and comparison of ELISA and LC-MS/MS for fumonisin analysis.
abstract: Dried Distiller's Grains with Solubles (DDGS), a by-product of bio-ethanol production from maize and other cereals, is increasingly used as a feed additive. In this study, five Fusarium toxins, including fumonisin B1 (FB1), fumonisin B2 (FB2), deoxynivalenol (DON), zearalenone (ZEN) and beauvericin (BEA) were quantified by LC-MS/MS in 59 corn-DDGS samples. In addition, the fumonisin level in 30 randomly selected-samples was compared using an ELISA detection technique. No sample was free from mycotoxin contamination, and 50.8 % of the samples were co-contaminated with all five mycotoxins. Moreover, toxin levels were generally high, with mean levels of 9 mg kg(-1) FB1, 6 mg kg(-1) FB2, 1.2 mg kg(-1) DON, 0.9 mg kg(-1) ZEN, and 0.35 mg kg(-1) BEA. Maximum levels for FB1 (143 mg kg(-1)) and FB2 (125 mg kg(-1)) are of acute toxicological relevance. The ELISA method had a tendency to underestimate the fumonisin content when compared with LC-MS/MS. Finally, this is the first reported beauvericin contamination in corn-DDGS.
authors.affiliation: Department of Pharmacology, Faculty of Veterinary Medicine, Kasetsart University, Bangkok, 10900, Thailand, natthasitt@yahoo.com.
authors.forename: Natthasit
authors.initials: N
authors.lastname: Tansakul
authors.forename: Prakorn
authors.initials: P
authors.lastname: Jala
authors.forename: Sudtisa
authors.initials: S
authors.lastname: Laopiem
authors.forename: Prapeuk
authors.initials: P
authors.lastname: Tangmunkhong
authors.forename: Sasithorn
authors.initials: S
authors.lastname: Limsuwan
mesh_terms: D000821:Animal Feed / Q000032:analysis*; D002853:Chromatography, Liquid; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005506:Food Contamination / Q000032:analysis*; D005670:Fusarium / Q000737:chemistry*; D009183:Mycotoxins / Q000032:analysis*; D053719:Tandem Mass Spectrometry / Q000379:methods; D013785:Thailand; D003313:Zea mays / Q000382:microbiology*
chemical_list: D009183:Mycotoxins
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