title: Cut, via CrebA, transcriptionally regulates the COPII secretory pathway to direct dendrite development in Drosophila.
abstract: Dendrite development is crucial in the formation of functional neural networks. Recent studies have provided insights into the involvement of secretory transport in dendritogenesis, raising the question of how the secretory pathway is controlled to direct dendritic elaboration. Here, we identify a functional link between transcriptional regulatory programs and the COPII secretory machinery in driving dendrite morphogenesis in Drosophila dendritic arborization (da) sensory neurons. MARCM analyses and gain-of-function studies reveal cell-autonomous requirements for the COPII coat protein Sec31 in mediating da neuron dendritic homeostasis. We demonstrate that the homeodomain protein Cut transcriptionally regulates Sec31 in addition to other components of COPII secretory transport, to promote dendrite elaboration, accompanied by increased satellite secretory endoplasmic reticulum (ER) and Golgi outposts primarily localized to dendritic branch points. We further establish a novel functional role for the transcription factor CrebA in regulating dendrite development and show that Cut initiates a gene expression cascade through CrebA that coordinately affects the COPII machinery to mediate dendritic morphology.
authors.affiliation: School of Systems Biology, George Mason University, Manassas, VA 20120, USA.
authors.forename: Srividya Chandramouli
authors.initials: SC
authors.lastname: Iyer
authors.forename: Eswar P
authors.initials: EP
authors.lastname: Ramachandran Iyer
authors.forename: Ramakrishna
authors.initials: R
authors.lastname: Meduri
authors.forename: Myurajan
authors.initials: M
authors.lastname: Rubaharan
authors.forename: Aravinda
authors.initials: A
authors.lastname: Kuntimaddi
authors.forename: Madhu
authors.initials: M
authors.lastname: Karamsetty
authors.forename: Daniel N
authors.initials: DN
authors.lastname: Cox
mesh_terms: D000818:Animals; D022181:COP-Coated Vesicles / Q000378:metabolism*; D003712:Dendrites / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D004330:Drosophila / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental; D021381:Protein Transport; D055571:Secretory Pathway; D014158:Transcription, Genetic
chemical_list: D029721:Drosophila Proteins
keywords: COPII secretory pathway; Dendrite; Transcriptional regulation
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