title: Bulky dna adducts in cord blood, maternal fruit-and-vegetable consumption, and birth weight in a European mother-child study (NewGeneris).
abstract: BACKGROUND
  Tobacco-smoke, airborne, and dietary exposures to polycyclic aromatic hydrocarbons (PAHs) have been associated with reduced prenatal growth. Evidence from biomarker-based studies of low-exposed populations is limited. Bulky DNA adducts in cord blood reflect the prenatal effective dose to several genotoxic agents including PAHs.
  
  
  OBJECTIVE
  We estimated the association between bulky DNA adduct levels and birth weight in a multicenter study and examined modification of this association by maternal intake of fruits and vegetables during pregnancy.
  
  
  METHODS
  Pregnant women from Denmark, England, Greece, Norway, and Spain were recruited in 2006-2010. Adduct levels were measured by the 32P-postlabeling technique in white blood cells from 229 mothers and 612 newborns. Maternal diet was examined through questionnaires.
  
  
  RESULTS
  Adduct levels in maternal and cord blood samples were similar and positively correlated (median, 12.1 vs. 11.4 adducts in 108 nucleotides; Spearman rank correlation coefficient = 0.66, p < 0.001). Cord blood adduct levels were negatively associated with birth weight, with an estimated difference in mean birth weight of -129 g (95% CI: -233, -25 g) for infants in the highest versus lowest tertile of adducts. The negative association with birth weight was limited to births in Norway, Denmark, and England, the countries with the lowest adduct levels, and was more pronounced in births to mothers with low intake of fruits and vegetables (-248 g; 95% CI: -405, -92 g) compared with those with high intake (-58 g; 95% CI: -206, 90 g).
  
  
  CONCLUSIONS
  Maternal exposure to genotoxic agents that induce the formation of bulky DNA adducts may affect intrauterine growth. Maternal fruit and vegetable consumption may be protective.
authors.affiliation: Centre for Research in Environmental Epidemiology (CREAL), Barcelona, Spain.
authors.forename: Marie
authors.initials: M
authors.lastname: Pedersen
authors.forename: Bernadette
authors.initials: B
authors.lastname: Schoket
authors.forename: Roger W
authors.initials: RW
authors.lastname: Godschalk
authors.forename: John
authors.initials: J
authors.lastname: Wright
authors.forename: Hans
authors.initials: H
authors.lastname: von Stedingk
authors.forename: Margareta
authors.initials: M
authors.lastname: Törnqvist
authors.forename: Jordi
authors.initials: J
authors.lastname: Sunyer
authors.forename: Jeanette K
authors.initials: JK
authors.lastname: Nielsen
authors.forename: Domenico F
authors.initials: DF
authors.lastname: Merlo
authors.forename: Michelle A
authors.initials: MA
authors.lastname: Mendez
authors.forename: Helle M
authors.initials: HM
authors.lastname: Meltzer
authors.forename: Viktória
authors.initials: V
authors.lastname: Lukács
authors.forename: Anette
authors.initials: A
authors.lastname: Landström
authors.forename: Soterios A
authors.initials: SA
authors.lastname: Kyrtopoulos
authors.forename: Katalin
authors.initials: K
authors.lastname: Kovács
authors.forename: Lisbeth E
authors.initials: LE
authors.lastname: Knudsen
authors.forename: Margaretha
authors.initials: M
authors.lastname: Haugen
authors.forename: Laura J
authors.initials: LJ
authors.lastname: Hardie
authors.forename: Kristine B
authors.initials: KB
authors.lastname: Gützkow
authors.forename: Sarah
authors.initials: S
authors.lastname: Fleming
authors.forename: Eleni
authors.initials: E
authors.lastname: Fthenou
authors.forename: Peter B
authors.initials: PB
authors.lastname: Farmer
authors.forename: Aina
authors.initials: A
authors.lastname: Espinosa
authors.forename: Leda
authors.initials: L
authors.lastname: Chatzi
authors.forename: Gunnar
authors.initials: G
authors.lastname: Brunborg
authors.forename: Nigel J
authors.initials: NJ
authors.lastname: Brady
authors.forename: Maria
authors.initials: M
authors.lastname: Botsivali
authors.forename: Khelifa
authors.initials: K
authors.lastname: Arab
authors.forename: Lívia
authors.initials: L
authors.lastname: Anna
authors.forename: Jan
authors.initials: J
authors.lastname: Alexander
authors.forename: Silvia
authors.initials: S
authors.lastname: Agramunt
authors.forename: Jos C
authors.initials: JC
authors.lastname: Kleinjans
authors.forename: Dan
authors.initials: D
authors.lastname: Segerbäck
authors.forename: Manolis
authors.initials: M
authors.lastname: Kogevinas
mesh_terms: D001724:Birth Weight / Q000502:physiology*; D018736:DNA Adducts / Q000097:blood*; D004032:Diet; D005260:Female; D005312:Fetal Blood / Q000737:chemistry*; D005638:Fruit; D006801:Humans; D018811:Maternal Exposure / Q000009:adverse effects; D011084:Polycyclic Aromatic Hydrocarbons / Q000633:toxicity; D014675:Vegetables
chemical_list: D018736:DNA Adducts; D011084:Polycyclic Aromatic Hydrocarbons
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