title: ramR mutations affecting fluoroquinolone susceptibility in epidemic multidrug-resistant Salmonella enterica serovar Kentucky ST198.
abstract: A screening for non-target mutations affecting fluoroquinolone susceptibility was conducted in epidemic multidrug-resistant Salmonella enterica serovar Kentucky ST198. Among a panel of representative isolates (n = 27), covering the epidemic, only three showed distinct mutations in ramR resulting in enhanced expression of genes encoding the AcrAB-TolC efflux system and low increase in ciprofloxacin MIC. No mutations were detected in other regulatory regions of this efflux system. Ciprofloxacin resistance in serovar Kentucky ST198 is thus currently mainly due to multiple target gene mutations.
authors.affiliation: INRA, UMR1282 Infectiologie et Santé Publique Nouzilly, France ; Université François Rabelais de Tours, UMR1282 Infectiologie et Santé Publique Tours, France.
authors.forename: Sylvie
authors.initials: S
authors.lastname: Baucheron
authors.forename: Simon
authors.initials: S
authors.lastname: Le Hello
authors.forename: Benoît
authors.initials: B
authors.lastname: Doublet
authors.forename: Etienne
authors.initials: E
authors.lastname: Giraud
authors.forename: François-Xavier
authors.initials: FX
authors.lastname: Weill
authors.forename: Axel
authors.initials: A
authors.lastname: Cloeckaert
keywords: Salmonella; ciprofloxacin resistance; efflux pump; ram; regulation
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