title: Molecular detection and conventional identification of leishmania species in reservoir hosts of zoonotic cutaneous leishmaniasis in fars province, South of iran.
abstract: BACKGROUND
  The objectives of our research were to search for Leishmania species in rodents in Fars province, south of Iran, and to compare molecular with conventional methods for detecting these parasites.
  
  
  METHODS
  Rodents were captured using live traps and screened for Leishmania species using molecular and conventional methods, including the taking of smears from each ear. Nested PCR was employed to detect Leishmania in rodents by amplifying a region of the ribosomal RNA amplicon of Leishmania (ITS1-5.8S rRNA-ITS2) that is species-specific by DNA sequence.
  
  
  RESULTS
  Totally, 122 rodents were captured. Leishmania parasites were detected using the nested PCR and three conventional methods (direct smear, NNN culture and Balb/C inoculation. 41 (33.6%) out of 122 rodents had Leishmania infections (34 Meriones lybicus and 7 M. persicus). All PCR products of the ITS-rDNA gene were sequenced. Sequence analysis revealed that 28 out of 41 positive samples were Leishmania major. Thirteen sequences were unreadable and therefore not identified.
  
  
  CONCLUSIONS
  At least two gerbil species common in Fars ZCL foci, M. lybicus and M. persicus, are acquiring infections of L. major and may be reservoir hosts of one predominant parasite haplotype. Most infections were detected molecularly not by conventional methods, because most rodents died in the traps.
authors.affiliation: Molecular Systematic Laboratory, Parasitology Department, Pasteur Institute of Iran, Tehran, Iran ; Medical Faculty, Ilam University of Medical Sciences, Ilam, Iran.
authors.forename: A
authors.initials: A
authors.lastname: Mirzaei
authors.forename: S
authors.initials: S
authors.lastname: Rouhani
authors.forename: Pa
authors.initials: P
authors.lastname: Kazerooni
authors.forename: M
authors.initials: M
authors.lastname: Farahmand
authors.forename: P
authors.initials: P
authors.lastname: Parvizi
keywords: ITS-ribosomal DNA; Iran; Leishmania major; Nested PCRs; Rodents
references.citation: Acta Trop. 1995 Aug;59(4):279-82
references.pmid: 8533663
references.citation: Bull Soc Pathol Exot. 2010 May;103(2):84-9
references.pmid: 20390397
references.citation: Acta Trop. 2000 May 31;75(3):301-7
references.pmid: 10838213
references.citation: Annu Rev Entomol. 2013;58:227-50
references.pmid: 23317043
references.citation: Trans R Soc Trop Med Hyg. 1996 Sep-Oct;90(5):503-4
references.pmid: 8944255
references.citation: Trans R Soc Trop Med Hyg. 2013 Jun;107(6):356-62
references.pmid: 23604865
references.citation: Parasitology. 2011 Apr;138(4):405-25
references.pmid: 21078222
references.citation: Vector Borne Zoonotic Dis. 2011 Feb;11(2):145-50
references.pmid: 20575646
references.citation: Trans R Soc Trop Med Hyg. 1968;62(4):534-42
references.pmid: 5691462
references.citation: Ann Trop Med Parasitol. 2003 Dec;97(8):811-6
references.pmid: 14754493
references.citation: PLoS One. 2012;7(5):e35671
references.pmid: 22693548
references.citation: East Mediterr Health J. 2006 May-Jul;12(3-4):474-7
references.pmid: 17037718
references.citation: Mol Biochem Parasitol. 1995 Jul;73(1-2):145-55
references.pmid: 8577322
references.citation: Ann Trop Med Parasitol. 2006 Jan;100(1):63-8
references.pmid: 16417715
references.citation: Exp Parasitol. 2011 Dec;129(4):375-80
references.pmid: 21945269
references.citation: Parasitol Res. 2008 Nov;103(6):1273-8
references.pmid: 18791741
references.citation: Trans R Soc Trop Med Hyg. 1975;69(5-6):517-9
references.pmid: 1228991
references.citation: East Mediterr Health J. 2004 Jul-Sep;10(4-5):591-9
references.pmid: 16335651
references.citation: J Clin Microbiol. 2006 Apr;44(4):1435-9
references.pmid: 16597873
references.citation: Acta Trop. 2005 Jan;93(1):75-83
references.pmid: 15589800
references.citation: Trop Med Int Health. 2012 Nov;17(11):1335-44
references.pmid: 22947226
references.citation: PLoS Negl Trop Dis. 2010 Jan 12;4(1):e581
references.pmid: 20069036
references.citation: Ann Trop Med Parasitol. 2001 Dec;95(8):811-9
references.pmid: 11784435
references.citation: Trop Med Int Health. 2008 Sep;13(9):1159-71
references.pmid: 18631311
references.citation: Iran J Arthropod Borne Dis. 2011;5(2):69-76
references.pmid: 22808420