title: West Nile Virus equine serosurvey in the Czech and Slovak republics.
abstract: A serological survey for West Nile virus (WNV) infection involved 395 horses from 43 administrative districts of the Czech Republic (163 animals) and 29 districts of Slovakia (232 animals), sampled between 2008 and 2011. Using a plaque-reduction neutralization microtest, antibodies to WNV were not detected in any horse from the Czech Republic, whereas 19 nonvaccinated horses from Slovakia had specific antibodies to WNV (no cross-reactions were observed with tick-borne encephalitis and Usutu flaviviruses in those animals). The seropositivity rate of nonvaccinated horses in Slovakia was 8.3% (95% confidence interval [CI] 4.7-11.9%), and autochthonous local infection with WNV occurred at least in 11, i.e., 4.8% (95% CI 2.0-7.6%) of the animals. All seropositive horses lived in six lowland districts of southern Slovakia; overall, 15.1% (95% CI 8.8-21.4%) of 126 nonvaccinated horses were seropositive in those districts, situated relatively closely to the border with Hungary, i.e., the country where WNV disease cases have been reported in birds, horses and humans since 2003.
authors.affiliation: 1 Institute of Vertebrate Biology , v.v.i., Academy of Sciences, 60365 Brno, Czech Republic .
authors.forename: Zdenek
authors.initials: Z
authors.lastname: Hubálek
authors.forename: Eva
authors.initials: E
authors.lastname: Ludvíková
authors.forename: Petr
authors.initials: P
authors.lastname: Jahn
authors.forename: František
authors.initials: F
authors.lastname: Treml
authors.forename: Ivo
authors.initials: I
authors.lastname: Rudolf
authors.forename: Petra
authors.initials: P
authors.lastname: Svobodová
authors.forename: Silvie
authors.initials: S
authors.lastname: Šikutová
authors.forename: Lenka
authors.initials: L
authors.lastname: Betášová
authors.forename: Jozef
authors.initials: J
authors.lastname: Bíreš
authors.forename: Miroslav
authors.initials: M
authors.lastname: Mojžíš
authors.forename: Martin
authors.initials: M
authors.lastname: Tinák
authors.forename: Martin
authors.initials: M
authors.lastname: Boldižár
authors.forename: Gabriela
authors.initials: G
authors.lastname: Citsoňová
authors.forename: Zuzana
authors.initials: Z
authors.lastname: Staššíková
mesh_terms: D000818:Animals; D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral / Q000097:blood*; D018153:Czech Republic / Q000453:epidemiology; D005260:Female; D006734:Horse Diseases / Q000453:epidemiology* / Q000821:virology; D006736:Horses; D006801:Humans; D008297:Male; D016036:Seroepidemiologic Studies; D018154:Slovakia / Q000453:epidemiology; D014901:West Nile Fever / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D014902:West Nile virus / Q000276:immunology* / Q000302:isolation & purification
chemical_list: D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral
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