title: Prozone effects in microscopic agglutination tests for leptospirosis in the sera of mice infected with the pathogenic Leptospira interrogans serovar Canicola.
abstract: Mice experimentally infected with a pathogenic strain of Leptospira interrogans serovar Canicola produced false negative results (prozone effect) in a microscopic agglutination test (MAT). This prozone effect occurred in several serum samples collected at different post-infection times, but it was more prominent in samples collected from seven-42 days post-infection and for 1:50 and 1:100 sample dilutions. This phenomenon was correlated with increased antibody titres in the early post-infection phase. While prozone effects are often observed in serological agglutination assays for the diagnosis of animal brucellosis and human syphilis, they are not widely reported in leptospirosis MATs.
authors.affiliation: Centro de Laboratório Regional XI, Instituto Adolfo Lutz, Sorocaba, SP, Brasil. fhsvet@bol.com.br
authors.forename: Fabio Hiroto
authors.initials: FH
authors.lastname: Shimabukuro
authors.forename: Veruska Maia da
authors.initials: VM
authors.lastname: Costa
authors.forename: Rodrigo Costa da
authors.initials: RC
authors.lastname: Silva
authors.forename: Hélio
authors.initials: H
authors.lastname: Langoni
authors.forename: Aristeu Vieira
authors.initials: AV
authors.lastname: da Silva
authors.forename: Lídia Raquel
authors.initials: LR
authors.lastname: de Carvalho
authors.forename: Paulo Francisco
authors.initials: PF
authors.lastname: Domingues
mesh_terms: D000372:Agglutination Tests; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D041341:Leptospira interrogans serovar canicola / Q000276:immunology*; D007922:Leptospirosis / Q000175:diagnosis* / Q000382:microbiology; D051379:Mice
chemical_list: D000907:Antibodies, Bacterial
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