title: Radiation therapy induces the DNA damage response in peripheral blood.
abstract: Stereotactic body radiation therapy (SBRT) is a radiotherapy modality that delivers highly conformal, ablative doses to a well-defined target. Here, using a semiquantitative multiplexed assay to analyze ATM and H2AX phosphorylation, we show that ATM kinase activity in peripheral blood mononuclear cells is induced following SBRT. This observation of a systemic ATM kinase-dependent DNA damage response in the peripheral blood is unprecedented and promotes the use of ATM serine-1981 phosphorylation as a predictive biomarker for DNA damaging modalities and ATM inhibitors.
authors.affiliation: Department of Radiation Oncology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA.
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Bakkenist
authors.forename: R Kenneth
authors.initials: RK
authors.lastname: Czambel
authors.forename: David A
authors.initials: DA
authors.lastname: Clump
authors.forename: Joel S
authors.initials: JS
authors.lastname: Greenberger
authors.forename: Jan H
authors.initials: JH
authors.lastname: Beumer
authors.forename: John C
authors.initials: JC
authors.lastname: Schmitz
mesh_terms: D064007:Ataxia Telangiectasia Mutated Proteins / Q000097:blood; D014408:Biomarkers, Tumor / Q000097:blood; D045744:Cell Line, Tumor; D004249:DNA Damage; D004273:DNA, Neoplasm / Q000097:blood* / Q000528:radiation effects*; D019073:HT29 Cells; D006657:Histones / Q000097:blood; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000528:radiation effects; D009369:Neoplasms / Q000097:blood / Q000235:genetics* / Q000601:surgery*; D010766:Phosphorylation; D016634:Radiosurgery / Q000009:adverse effects* / Q000379:methods
chemical_list: D014408:Biomarkers, Tumor; D004273:DNA, Neoplasm; C468783:H2AX protein, human; D006657:Histones; C576324:ATM protein, human; D064007:Ataxia Telangiectasia Mutated Proteins
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