title: The diversity of Rhizobia, Sinorhizobia and novel non-Rhizobial Paenibacillus nodulating wild herbaceous legumes.
abstract: The objective of the present study was to isolate and characterize nodulating bacteria associated with wild legumes. For this purpose, we recovered twenty isolates from root nodules of five wild legume species: Melilotus alles, Melilotus officinalis, Trifolium pratense, Trifolium repens and Medicago sp. Most of the isolates were morphologically analogous with only few exceptions in colony shape, appearance and incubation time. All isolates were Gram negative except T.P2-4. Random amplification of polymorphic DNA showed genetic variation among isolates. The 16S rRNA sequence analysis revealed these isolates as Rhizobium, Sinorhizobium and Paenibacillus. Each of these was also screened for nod D and nod F genes with marked variation at these loci; however, the nucleotide sequence analysis confirmed the presence of nod genes. The assignment of strains to their hosts revealed a unique symbiotic association of Paenibacillus sp. nodulating T .pratense which is being reported here for the first time.
authors.affiliation: Department of Plant Sciences, Quaid-i-Azam University, Islamabad 45320, Pakistan.
authors.forename: Sadia
authors.initials: S
authors.lastname: Latif
authors.forename: Samiullah
authors.initials: S
authors.lastname: Khan
authors.forename: Muhammad
authors.initials: M
authors.lastname: Naveed
authors.forename: Ghulam
authors.initials: G
authors.lastname: Mustafa
authors.forename: Tasmia
authors.initials: T
authors.lastname: Bashir
authors.forename: Abdul Samad
authors.initials: AS
authors.lastname: Mumtaz
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D007887:Fabaceae / Q000382:microbiology* / Q000502:physiology; D014644:Genetic Variation; D056507:Paenibacillus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D010802:Phylogeny; D018517:Plant Roots / Q000382:microbiology; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012231:Rhizobium / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D020579:Sinorhizobium / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D013559:Symbiosis
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S