title: Insertional mutagenesis using Tnt1 retrotransposon in potato.
abstract: Insertional mutagenesis using transfer DNA or transposable elements, which is an important tool in functional genomics and is well established in several crops, has not been developed in potato (Solanum tuberosum). Here, we report the application of the tobacco (Nicotiana tabacum) Tnt1 retrotransposon as an insertional mutagen in potato. The Tnt1 retrotransposon was introduced into a highly homozygous and self-compatible clone, 523-3, of the diploid wild potato species Solanum chacoense. Transposition of the Tnt1 elements introduced into 523-3 can be efficiently induced by tissue culture. Tnt1 preferentially inserted into genic regions in the potato genome and the insertions were stable during sexual reproduction, making Tnt1 an ideal mutagen in potato. Several distinct phenotypes associated with plant stature and leaf morphology were discovered in mutation screening from a total of 38 families derived from Tnt1-containing lines. We demonstrate that the insertional mutagenesis system based on Tnt1 and the 523-3 clone can be expanded to the genome-wide level to potentially tag every gene in the potato genome.
authors.affiliation: Department of Horticulture, University of Wisconsin-Madison, Madison, Wisconsin 53706, USA.
authors.forename: Saowapa
authors.initials: S
authors.lastname: Duangpan
authors.forename: Wenli
authors.initials: W
authors.lastname: Zhang
authors.forename: Yufang
authors.initials: Y
authors.lastname: Wu
authors.forename: Shelley H
authors.initials: SH
authors.lastname: Jansky
authors.forename: Jiming
authors.initials: J
authors.lastname: Jiang
mesh_terms: D018745:Genome, Plant; D016254:Mutagenesis, Insertional / Q000379:methods*; D018626:Retroelements / Q000235:genetics*; D011198:Solanum tuberosum / Q000235:genetics*; D014026:Nicotiana / Q000235:genetics
chemical_list: D018626:Retroelements
references.citation: New Phytol. 2013 Mar;197(4):1250-1261
references.pmid: 23278348
references.citation: Plant Physiol. 2009 Nov;151(3):1114-29
references.pmid: 19710231
references.citation: Plant Cell Rep. 2005 Apr;24(1):52-8
references.pmid: 15692822
references.citation: Plant Physiol. 2011 Aug;156(4):2207-24
references.pmid: 21685176
references.citation: Methods Mol Biol. 2011;678:179-90
references.pmid: 20931380
references.citation: Plant Cell. 2011 Jun;23(6):2125-42
references.pmid: 21719692
references.citation: PLoS One. 2012;7(4):e36347
references.pmid: 22558443
references.citation: BMC Plant Biol. 2008 Jan 23;8:8
references.pmid: 18215301
references.citation: Mol Genet Genomics. 2001 Mar;265(1):32-42
references.pmid: 11370870
references.citation: Plant J. 2004 Aug;39(3):450-64
references.pmid: 15255873
references.citation: Plant Cell. 1999 Dec;11(12):2283-90
references.pmid: 10590158
references.citation: Plant Physiol. 2000 Apr;122(4):1003-13
references.pmid: 10759496
references.citation: Nature. 2011 Jul 10;475(7355):189-95
references.pmid: 21743474
references.citation: Plant Physiol. 2013 Jan;161(1):36-47
references.pmid: 23124322
references.citation: Plant Physiol. 2011 Nov;157(3):1483-96
references.pmid: 21957014
references.citation: Plant J. 2003 Apr;34(1):95-106
references.pmid: 12662312
references.citation: New Phytol. 2012 Jul;195(1):71-84
references.pmid: 22530598
references.citation: Plant J. 2000 Jul;23(2):291-304
references.pmid: 10929123
references.citation: Plant J. 2000 Jun;22(6):561-70
references.pmid: 10886776
references.citation: Plant J. 2012 Feb;69(4):720-30
references.pmid: 22014259
references.citation: Plant Physiol. 2007 May;144(1):18-31
references.pmid: 17351058
references.citation: Science. 2003 Aug 1;301(5633):653-7
references.pmid: 12893945
references.citation: Genome Res. 2002 Apr;12(4):656-64
references.pmid: 11932250
references.citation: Nature. 1989 Jan 26;337(6205):376-80
references.pmid: 2536143
references.citation: Plant Physiol. 2008 Jul;147(3):1264-78
references.pmid: 18467467
references.citation: Plant Cell. 1996 Apr;8(4):725-34
references.pmid: 8624443
references.citation: Plant J. 2008 Apr;54(2):335-47
references.pmid: 18208518
references.citation: EMBO J. 1995 May 15;14(10):2364-73
references.pmid: 7774594
references.citation: BMC Genomics. 2011 Jun 09;12:302
references.pmid: 21658273
references.citation: Plant J. 1991 Jul;1(1):59-69
references.pmid: 1668965
references.citation: Plant J. 2010 Jul 1;63(1):100-14
references.pmid: 20408998