title: The modification experimental study in vivo of nano-bone gelatin.
abstract: OBJECTIVE
  To study the feasibility of therapy using nano-bone gelatin to comminuted fracture by animal experiment.
  
  
  METHODS
  The animal models of transverse fracture were made on bilateral ulnas of 45 New Zealand white rabbits, which were divided into experimental group (repair with nano-bone gelatin), control group (repair with traditional medical glue), and blank group (unrepaired) randomly. The reconstruction effect in each group was evaluated using X-ray examination, MicroCT scanning, histopathology observation, and biomechanical test postoperation regularly.
  
  
  RESULTS
  On 12th week, the fractures in experimental and blank group get the marrow cavity completely unobstructed and good bone union; however, in control group, the fracture line slightly blurred with the marrow cavity not fully unobstructed; 6 weeks later, observation of bony calluses through MircoCT: experimental group, 68.5 ± 2.71%; blank group, 69.19 ± 2.3%; and control group, 49.35 ± 3.56%, there were no significant difference between the two groups (P > 0.05). The control group obviously showed worse bone union than the former two (P < 0.05). The histopathological examination shows that the bony calluses of experimental group are similar to those of the blank group; however, gelatin degraded slowly in control group with delayed union; on the 12th week, biomechanical test shows that the blank and experiment groups had basically same average bending strength values which had no significant difference (P > 0.05) and obviously were higher than those of the control group (P < 0.01).
  
  
  CONCLUSIONS
  The nano-bone gelatin won't lead to delayed union of fractures and may be beneficial to it, and so may be an ideal gelatin for fixing small fractures.
authors.affiliation: Anhui Medical College , Anhui P. R. China.
authors.forename: Wencui
authors.initials: W
authors.lastname: Li
authors.forename: Zhe
authors.initials: Z
authors.lastname: Zhao
authors.forename: Jianyi
authors.initials: J
authors.lastname: Xiong
authors.forename: Yanjun
authors.initials: Y
authors.lastname: Zeng
mesh_terms: D000269:Adhesives / Q000737:chemistry* / Q000494:pharmacology*; D000818:Animals; D001696:Biomechanical Phenomena / Q000187:drug effects; D050723:Fractures, Bone / Q000000981:diagnostic imaging / Q000473:pathology; D005780:Gelatin / Q000737:chemistry* / Q000494:pharmacology*; D008297:Male; D049329:Nanostructures; D009929:Organ Size / Q000187:drug effects; D011817:Rabbits; D014457:Ulna / Q000166:cytology / Q000000981:diagnostic imaging / Q000187:drug effects* / Q000473:pathology; D055114:X-Ray Microtomography
chemical_list: D000269:Adhesives; D005780:Gelatin
keywords: cyanoacrylate; fractures; gelatin; nanometer