title: Photosynthetic control of Arabidopsis leaf cytoplasmic translation initiation by protein phosphorylation.
abstract: Photosynthetic CO2 assimilation is the carbon source for plant anabolism, including amino acid production and protein synthesis. The biosynthesis of leaf proteins is known for decades to correlate with photosynthetic activity but the mechanisms controlling this effect are not documented. The cornerstone of the regulation of protein synthesis is believed to be translation initiation, which involves multiple phosphorylation events in Eukaryotes. We took advantage of phosphoproteomic methods applied to Arabidopsis thaliana rosettes harvested under controlled photosynthetic gas-exchange conditions to characterize the phosphorylation pattern of ribosomal proteins (RPs) and eukaryotic initiation factors (eIFs). The analyses detected 14 and 11 new RP and eIF phosphorylation sites, respectively, revealed significant CO2-dependent and/or light/dark phosphorylation patterns and showed concerted changes in 13 eIF phosphorylation sites and 9 ribosomal phosphorylation sites. In addition to the well-recognized role of the ribosomal small subunit protein RPS6, our data indicate the involvement of eIF3, eIF4A, eIF4B, eIF4G and eIF5 phosphorylation in controlling translation initiation when photosynthesis varies. The response of protein biosynthesis to the photosynthetic input thus appears to be the result of a complex regulation network involving both stimulating (e.g. RPS6, eIF4B phosphorylation) and inhibiting (e.g. eIF4G phosphorylation) molecular events.
authors.affiliation: Institut de Biologie des Plantes, CNRS UMR 8618, Saclay Plant Sciences, Université Paris-Sud, Orsay, France.
authors.forename: Edouard
authors.initials: E
authors.lastname: Boex-Fontvieille
authors.forename: Marlène
authors.initials: M
authors.lastname: Daventure
authors.forename: Mathieu
authors.initials: M
authors.lastname: Jossier
authors.forename: Michel
authors.initials: M
authors.lastname: Zivy
authors.forename: Michael
authors.initials: M
authors.lastname: Hodges
authors.forename: Guillaume
authors.initials: G
authors.lastname: Tcherkez
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D039642:Eukaryotic Initiation Factors / Q000378:metabolism*; D010766:Phosphorylation / Q000235:genetics / Q000502:physiology; D010788:Photosynthesis / Q000235:genetics / Q000502:physiology*; D018515:Plant Leaves / Q000235:genetics / Q000378:metabolism*; D014176:Protein Biosynthesis / Q000235:genetics / Q000502:physiology
chemical_list: D029681:Arabidopsis Proteins; D039642:Eukaryotic Initiation Factors
references.citation: Trends Plant Sci. 2011 Sep;16(9):489-98
references.pmid: 21684795
references.citation: Plant Cell. 1997 Dec;9(12):2291-300
references.pmid: 9437868
references.citation: Nat Protoc. 2009;4(4):484-94
references.pmid: 19300442
references.citation: Mol Syst Biol. 2009;5:314
references.pmid: 19888209
references.citation: Mol Cell Proteomics. 2008 Feb;7(2):347-69
references.pmid: 17934214
references.citation: Bioinformatics. 2004 Jun 12;20(9):1466-7
references.pmid: 14976030
references.citation: Curr Opin Plant Biol. 2012 Jun;15(3):301-7
references.pmid: 22305521
references.citation: Plant Physiol. 2011 Nov;157(3):1537-45
references.pmid: 21900482
references.citation: EMBO Rep. 2007 Sep;8(9):864-70
references.pmid: 17721444
references.citation: J Biol Chem. 2009 Jul 31;284(31):20615-28
references.pmid: 19509420
references.citation: Plant Mol Biol. 1996 Oct;32(1-2):107-44
references.pmid: 8980477
references.citation: J Biol Chem. 2011 Dec 2;286(48):41171-41182
references.pmid: 21994950
references.citation: J Biol Chem. 1991 Dec 5;266(34):23341-6
references.pmid: 1744128
references.citation: Proteomics. 2011 Sep;11(17):3572-7
references.pmid: 21751374
references.citation: Plant J. 2008 Jul;55(1):118-30
references.pmid: 18363781
references.citation: Biochimie. 2006 Jan;88(1):11-21
references.pmid: 16125835
references.citation: J Biol Chem. 1997 Jan 10;272(2):1046-53
references.pmid: 8995401
references.citation: Biochem Soc Trans. 2004 Aug;32(Pt 4):589-91
references.pmid: 15270683
references.citation: J Exp Bot. 2002 Apr;53(370):959-70
references.pmid: 11912238
references.citation: J Biol Chem. 2000 Jun 9;275(23):17452-62
references.pmid: 10747998
references.citation: BMC Plant Biol. 2008 Dec 24;8:134
references.pmid: 19108716
references.citation: Plant Physiol. 2005 Mar;137(3):848-62
references.pmid: 15734919
references.citation: Comp Funct Genomics. 2012;2012:287814
references.pmid: 22611336
references.citation: Plant Physiol. 1985 Feb;77(2):370-3
references.pmid: 16664060
references.citation: J Biol Chem. 2009 Jul 31;284(31):20602-14
references.pmid: 19509278
references.citation: Biochemistry. 2004 Jul 20;43(28):9092-7
references.pmid: 15248766
references.citation: Int Rev Cell Mol Biol. 2008;268:1-37
references.pmid: 18703402
references.citation: Trends Plant Sci. 2010 Sep;15(9):499-506
references.pmid: 20580304
references.citation: Mol Cell Biochem. 2011 Oct;356(1-2):241-4
references.pmid: 21750984
references.citation: Plant J. 2007 Sep;51(5):931-40
references.pmid: 17651370
references.citation: J Biol Chem. 2011 Dec 9;286(49):42566-42574
references.pmid: 21965660
references.citation: Biochim Biophys Acta. 1964 Sep 4;90:542-52
references.pmid: 14237862
references.citation: Plant Physiol. 2003 Aug;132(4):2086-97
references.pmid: 12913163
references.citation: PLoS One. 2011;6(12):e29307
references.pmid: 22195043
references.citation: Biochemistry. 2010 Sep 28;49(38):8251-65
references.pmid: 20795652
references.citation: Wiley Interdiscip Rev RNA. 2012 Mar-Apr;3(2):178-94
references.pmid: 22215505
references.citation: Plant Cell. 2006 Feb;18(2):477-90
references.pmid: 16377759
references.citation: Biochem Soc Trans. 2007 Dec;35(Pt 6):1634-7
references.pmid: 18031281
references.citation: Proc Natl Acad Sci U S A. 2008 Jul 15;105(28):9564-9
references.pmid: 18606994
references.citation: Nat Rev Mol Cell Biol. 2006 Nov;7(11):847-59
references.pmid: 16990790
references.citation: Mol Cell Proteomics. 2012 Oct;11(10):957-72
references.pmid: 22787273
references.citation: Cell. 1982 Sep;30(2):427-37
references.pmid: 7139709
references.citation: Nat Rev Mol Cell Biol. 2010 Feb;11(2):113-27
references.pmid: 20094052
references.citation: Funct Plant Biol. 2012 Sep;39(9):717-735
references.pmid: 32480823
references.citation: EMBO J. 2004 Apr 21;23(8):1761-9
references.pmid: 15071500
references.citation: J Biol Chem. 2010 Feb 26;285(9):6217-26
references.pmid: 20018887