title: Phospholamban antisense RNA improves SR Ca2+-ATPase activity and left ventricular function in STZ-induced diabetic rats.
abstract: OBJECTIVE
  To study the effect of phospholamban antisense RNA (asPLB) on sarcoplasmic reticulum Ca2+-ATPase activity and cardiac function in rats with diabetes mellitus (DM) mediated by recombinant adeno-associated virus (rAAV) vector.
  
  
  METHODS
  Six weeks after the induction of DM by streptozotocin injected intraperitoneally, the rats were divided into three groups, namely: DM-rAAV-asPLB group, DM-saline group and DM group (control group). The rats in the DM-rAAV-asPLB group were intramyocardially injected with rAAV-asPLB, the rats in the DM-saline group were injected with saline, and those in the control group did not receive any treatment. Six weeks after gene transfer, the expressions of PLB protein and PLB phosphorylation were detected by Western-blot, while the activity of sarcoplasmic reticulum (SR) Ca2+-ATPase and left ventricular function were measured.
  
  
  RESULTS
  The PLB protein expression level was significantly higher whereas the PLB phosphorylation, SR Ca2+-ATPase activity and left ventricular function were significantly lower in the DM-saline group than in the control group. No significant difference was found in PLB protein expression level, PLB phosphorylation or SR Ca2+-ATPase activity between the DM-rAAV-asPLB group and the control group. The left ventricular function in the DM-rAAV-asPLB group was poorer than in the control group and was better than in the DM-saline group.
  
  
  CONCLUSIONS
  rAAV-asPLB can down-regulate PLB protein expression and up-regulate PLB phosphorylation and SR Ca2+-ATPase activity, thus contributing to the improvement of in vivo left ventricular function.
authors.affiliation: Department of Emergency and ICU, Fourth Affiliated Hospital of Nanchang University, Nanchang 330003, Jiangxi, China.
authors.forename: Jiang
authors.initials: J
authors.lastname: Li
authors.forename: Bao Hui
authors.initials: BH
authors.lastname: Jia
authors.forename: Jian
authors.initials: J
authors.lastname: Sun
authors.forename: Xiao Liang
authors.initials: XL
authors.lastname: Lou
authors.forename: Shen Jiang
authors.initials: SJ
authors.lastname: Hu
mesh_terms: D000818:Animals; D002135:Calcium-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism; D008297:Male; D010766:Phosphorylation; D016372:RNA, Antisense / Q000008:administration & dosage*; D051381:Rats; D017208:Rats, Wistar; D053498:Sarcoplasmic Reticulum Calcium-Transporting ATPases / Q000378:metabolism*; D016277:Ventricular Function, Left / Q000187:drug effects*; D000099211:Phospholamban
chemical_list: D002135:Calcium-Binding Proteins; D016372:RNA, Antisense; D053498:Sarcoplasmic Reticulum Calcium-Transporting ATPases; D000099211:Phospholamban
keywords: Diabetes mellitus; Gene therapy; Phospholamban; Sarcoplasmic reticulum Ca2+-ATPase