title: Modification of the fatty acid composition of the erythrocyte membrane in patients with chronic respiratory diseases.
abstract: BACKGROUND
  Early preclinical diagnosis of COPD is urgent. We proposed that fatty acid composition of red blood cells may serve as a prognostic test for the complications in the chronic respiratory diseases. Fatty acid composition of the erythrocyte membranes in patients with chronic respiratory diseases (chronic bronchitis, CB, and stable chronic obstructive pulmonary disease, COPD) was studied. It was established that modification of the fatty acid composition in the erythrocyte membranes was unidirectional in both groups of patients.
  
  
  METHODS
  Patients with CB and stable COPD (group A, GOLD 1) (15 subjects in each group) were studied in clinic. The activity of the inflammatory process was evaluated by the phagocytic activity of neutrophils, cytokine levels and cytokine receptors in the blood serum (TNFα, sTNF-RI, bFGF, TGF-β, IL-8). Fatty acid (FA) composition of the erythrocyte membranes was analyzed by gas liquid chromatography. Statistical data processing was performed by the methods of descriptive statistics with Statistica 6.0.
  
  
  RESULTS
  In both groups (CB and COPD), a significant accumulation of the saturated FAs (14:0, 15:0, 18:0) was established. The amount of the arachidonic acid (20:4n-6) was increased by 13% (р < 0.05) in CB patients and by 41% (р < 0.001) in COPD patients, as compared with healthy persons. The elevated level of the PUFA n-6 in the erythrocytes membranes in patients with chronic respiratory diseases confirms that proinflammatory (leukotriene B4) and bronchospasm (prostaglandin D2) mediator substrates is increased. The level of the eicosapentaenoic acid (20:5n-3) was decreased by 32% (р < 0.05) in CB patients and 2-fold (р < 0.001) in COPD patients. The observed increase in the 20:4n-6/20:5n-3 ratio--1.5-fold (р < 0.001) in CB patients and 3-fold in COPD patients--can be a specific marker of the adverse course of the respiratory pathology and the chronic inflammatory development.
  
  
  CONCLUSIONS
  Chronic respiratory disease development is associated with the disturbance of the fatty acid composition in erythrocyte membranes and disbalance of the ratio between precursor of pro- and antiinflammatory eicosanoids.
authors.affiliation: Institute of Medical Climatology and Rehabilitative Treatment, Vladivostok Branch of the Far Eastern Center of Physiology and Pathology of Respiration of SB RAMN, Vladivostok, Russia.
authors.forename: Tatyana P
authors.initials: TP
authors.lastname: Novgorodtseva
authors.forename: Yulia K
authors.initials: YK
authors.lastname: Denisenko
authors.forename: Natalia V
authors.initials: NV
authors.lastname: Zhukova
authors.forename: Marina V
authors.initials: MV
authors.lastname: Antonyuk
authors.forename: Vera V
authors.initials: VV
authors.lastname: Knyshova
authors.forename: Tatyana A
authors.initials: TA
authors.lastname: Gvozdenko
mesh_terms: D000328:Adult; D029481:Bronchitis, Chronic / Q000097:blood* / Q000150:complications / Q000473:pathology; D015118:Eicosapentaenoic Acid / Q000378:metabolism*; D004910:Erythrocyte Membrane / Q000737:chemistry*; D005231:Fatty Acids, Unsaturated / Q000378:metabolism; D005260:Female; D006801:Humans; D007249:Inflammation / Q000097:blood* / Q000150:complications / Q000175:diagnosis; D016209:Interleukin-8 / Q000097:blood; D008297:Male; D008875:Middle Aged; D009504:Neutrophils / Q000737:chemistry / Q000378:metabolism; D029424:Pulmonary Disease, Chronic Obstructive / Q000097:blood* / Q000150:complications / Q000473:pathology; D016212:Transforming Growth Factor beta / Q000097:blood
chemical_list: D005231:Fatty Acids, Unsaturated; D016209:Interleukin-8; D016212:Transforming Growth Factor beta; D015118:Eicosapentaenoic Acid
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