title: DNA polymerases are error-prone at RecA-mediated recombination intermediates.
abstract: Genetic studies have suggested that Y-family translesion DNA polymerase IV (DinB) performs error-prone recombination-directed replication (RDR) under conditions of stress due to its ability to promote mutations during double-strand break (DSB) repair in growth-limited E. coli cells. In recent studies we have demonstrated that pol IV is preferentially recruited to D-loop recombination intermediates at stress-induced concentrations and is highly mutagenic during RDR in vitro. These findings verify longstanding genetic data that have implicated pol IV in promoting stress-induced mutagenesis at D-loops. In this Extra View, we demonstrate the surprising finding that A-family pol I, which normally exhibits high-fidelity DNA synthesis, is highly error-prone at D-loops like pol IV. These findings indicate that DNA polymerases are intrinsically error-prone at RecA-mediated D-loops and suggest that auxiliary factors are necessary for suppressing mutations during RDR in non-stressed proliferating cells.
authors.affiliation: Fels Institute for Cancer Research, Department of Biochemistry, Temple University School of Medicine, Philadelphia, PA, USA. richard.pomerantz@temple.edu
authors.forename: Richard T
authors.initials: RT
authors.lastname: Pomerantz
authors.forename: Myron F
authors.initials: MF
authors.lastname: Goodman
authors.forename: Michael E
authors.initials: ME
authors.lastname: O'Donnell
mesh_terms: D004247:DNA / Q000235:genetics; D004249:DNA Damage / Q000235:genetics*; D019951:DNA Polymerase beta / Q000378:metabolism*; D004261:DNA Replication / Q000235:genetics* / Q000502:physiology; D008957:Models, Genetic; D016296:Mutagenesis / Q000235:genetics*; D011938:Rec A Recombinases / Q000235:genetics / Q000378:metabolism*; D011995:Recombination, Genetic / Q000235:genetics*
chemical_list: C051802:triplex DNA; D004247:DNA; D011938:Rec A Recombinases; D019951:DNA Polymerase beta
keywords: DNA replication; adaptive mutagenesis; break-induced replication; double-strand break repair; homologous recombination; stress-induced mutagenesis
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