title: Is the atherosclerotic phenotype of preeclamptic placentas due to altered lipoprotein concentrations and placental lipoprotein receptors? Role of a small-for-gestational-age phenotype.
abstract: Atherosis of spiral arteries in uteroplacental beds from preeclamptic women resemble those of atherosclerosis, characterized by increased plasma lipids and lipoproteins. We hypothesized that: 1) lipoprotein receptors/transporters in the placenta would be upregulated in preeclampsia, associated with increased maternal and fetal lipoprotein concentrations; and 2) expression of these would be reduced in preeclamptic placentae from women delivering small-for-gestational-age (SGA) infants. Placental biopsies and maternal and umbilical serum samples were taken from 27 normotensive and 24 preeclamptic women. Maternal/umbilical cord serum LDL, HDL, total cholesterol, and triglycerides were measured. Placental mRNA expression of lipoprotein receptors/transporters were quantified using quantitative RT-PCR. Protein localization/expression of LDL receptor-related protein 1 (LRP-1) in the preeclamptic placentae with/without SGA was measured by immunohistochemistry. Placental mRNA expression of all genes except paraoxonase-1 (PON-1), microsomal triglyceride transfer protein (MTTP), and protein disulfide isomerase family A member 2 (PDIA2) were observed. No differences for any lipoprotein receptors/transporters were found between groups; however, in the preeclamptic group placental LRP-1 expression was lower in SGA delivering mothers (n = 7; P = 0.036). LRP-1 protein was localized around fetal vessels and Hofbauer cells. This is the first detailed study of maternal/fetal lipoprotein concentrations and placental lipoprotein receptor mRNA expression in normotensive and preeclamptic pregnancies. These findings do not support a role of altered lipid metabolism in preeclampsia, but may be involved in fetal growth.
authors.affiliation: Division of Women's Health, Women's Health Academic Centre, King's College London, London, UK.
authors.forename: Marta R
authors.initials: MR
authors.lastname: Hentschke
authors.forename: Carlos E
authors.initials: CE
authors.lastname: Poli-de-Figueiredo
authors.forename: Bartira E Pinheiro
authors.initials: BE
authors.lastname: da Costa
authors.forename: Lesia O
authors.initials: LO
authors.lastname: Kurlak
authors.forename: Paula J
authors.initials: PJ
authors.lastname: Williams
authors.forename: Hiten D
authors.initials: HD
authors.lastname: Mistry
mesh_terms: D000328:Adult; D050197:Atherosclerosis / Q000378:metabolism*; D001724:Birth Weight; D005260:Female; D005312:Fetal Blood / Q000378:metabolism; D015870:Gene Expression; D005865:Gestational Age; D006801:Humans; D007236:Infant, Small for Gestational Age; D008074:Lipoproteins / Q000097:blood*; D026503:Low Density Lipoprotein Receptor-Related Protein-1 / Q000235:genetics / Q000378:metabolism*; D010641:Phenotype; D010920:Placenta / Q000098:blood supply / Q000378:metabolism* / Q000473:pathology; D011225:Pre-Eclampsia / Q000378:metabolism*; D011247:Pregnancy; D055815:Young Adult
chemical_list: C475476:LRP1 protein, human; D008074:Lipoproteins; D026503:Low Density Lipoprotein Receptor-Related Protein-1
keywords: ATP binding cassette transporter A1; LDL receptor; LDL receptor-related protein 1; fetal growth; high density lipoprotein cholesterol; hypertension; lipids; low density liporotein cholesterol; scavenger receptor class B type 1; triglycerides
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