title: Oligoamines grafted hyperbranched polyether as high efficient and serum-tolerant gene vectors.
abstract: To develop low toxic, high efficient, and excellent serum-tolerant polycation gene delivery systems, a series of oligoamines grafted hyperbranched polyether (oligoamines-g-HBP) were synthesized by conjugating different oligoamines, including triethylenetetramine (TETA) and tetraethylenepentamine (TEPA), onto COOH-functionalized hyperbranched poly(3-ethyl-3-oxetanemethanol). It was found that oligoamines-g-HBP exhibited good buffering capacity, strong DNA binding and high resistance against protein adsorption. In vitro cytotoxicity measurement indicated that oligoamines-g-HBP had much lower cytotoxicity as compared with 25 kDa PEI. The transfection efficiency of TEPA-g-HBP/DNA complexes at a certain N/P ratio was significantly higher than that of 25 kDa PEI/DNA complexes. Interestingly, it was found that TEPA-g-HBP had much improved serum-tolerant capability as compared with 25 kDa PEI even when serum concentration was increased to 30%. Confocal laser images further showed that the amount of YOYO-1 labeled DNA in nuclei got increased with increasing the number of secondary amino ethylene groups in oligoamines-g-HBP. The oligoamines-g-HBP presented great potential as gene delivery vectors for further clinical applications.
authors.affiliation: Key Laboratory of Biomedical Polymers of Ministry of Education & Department of Chemistry, Wuhan University, Wuhan 430072, PR China.
authors.forename: Yun-Xia
authors.initials: YX
authors.lastname: Sun
authors.affiliation: Key Laboratory of Biomedical Polymers of Ministry of Education & Department of Chemistry, Wuhan University, Wuhan 430072, PR China.
authors.forename: Bin
authors.initials: B
authors.lastname: Yang
authors.affiliation: Key Laboratory of Biomedical Polymers of Ministry of Education & Department of Chemistry, Wuhan University, Wuhan 430072, PR China.
authors.forename: Si
authors.initials: S
authors.lastname: Chen
authors.affiliation: Key Laboratory of Biomedical Polymers of Ministry of Education & Department of Chemistry, Wuhan University, Wuhan 430072, PR China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Lei
authors.affiliation: Key Laboratory of Biomedical Polymers of Ministry of Education & Department of Chemistry, Wuhan University, Wuhan 430072, PR China.
authors.forename: Jun
authors.initials: J
authors.lastname: Feng
authors.affiliation: Department of Cardiovascular Surgery, Union Hospital, Huazhong University of Science and Technology, Wuhan 430022, PR China.
authors.forename: Xue-Feng
authors.initials: XF
authors.lastname: Qiu
authors.affiliation: Department of Cardiovascular Surgery, Union Hospital, Huazhong University of Science and Technology, Wuhan 430022, PR China.
authors.forename: Nian-Guo
authors.initials: NG
authors.lastname: Dong
authors.affiliation: Key Laboratory of Biomedical Polymers of Ministry of Education & Department of Chemistry, Wuhan University, Wuhan 430072, PR China.
authors.forename: Ren-Xi
authors.initials: RX
authors.lastname: Zhuo
authors.affiliation: Key Laboratory of Biomedical Polymers of Ministry of Education & Department of Chemistry, Wuhan University, Wuhan 430072, PR China. Electronic address: xz-zhang@whu.edu.cn.
authors.forename: Xian-Zheng
authors.initials: XZ
authors.lastname: Zhang
mesh_terms: D000327:Adsorption; D000588:Amines / Q000737:chemistry*; D000818:Animals; D002021:Buffers; D002417:Cattle; D004247:DNA / Q000378:metabolism; D024202:Electrophoretic Mobility Shift Assay; D000431:Ethanol / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry; D005822:Genetic Vectors / Q000378:metabolism*; D057809:HEK293 Cells; D006367:HeLa Cells; D006801:Humans; D042541:Intracellular Space / Q000378:metabolism; D008156:Luciferases / Q000378:metabolism; D008970:Molecular Weight; D010316:Particle Size; D011094:Polyethyleneimine / Q000737:chemistry; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry*; D044967:Serum / Q000378:metabolism*; D012710:Serum Albumin, Bovine / Q000378:metabolism; D055672:Static Electricity; D014162:Transfection
chemical_list: D000588:Amines; D002021:Buffers; D011108:Polymers; C548834:poly(3-ethyl-3-oxetanemethanol); D012710:Serum Albumin, Bovine; D000431:Ethanol; D011094:Polyethyleneimine; D004247:DNA; D008156:Luciferases
keywords: Gene delivery; Hyperbranched polycation; Oligoamines; Protein adsorption; Serum-tolerant capability