title: Atomic force microscopy observation of lipopolysaccharide-induced cardiomyocyte cytoskeleton reorganization.
abstract: We applied atomic force microscopy (AFM) to observe lipopolysaccharide (LPS)-induced intracellular cytoskeleton reorganization in primary cardiomyocytes from neonatal mouse. The nonionic detergent Triton X-100 was used to remove the membrane, soluble proteins, and organelles from the cell. The remaining cytoskeleton can then be directly visualized by AFM. Using three-dimensional technique of AFM, we were able to quantify the changes of cytoskeleton by the "density" and total "volume" of the cytoskeleton fibers. Compared to the control group, the density of cytoskeleton was remarkably decreased and the volume of cytoskeleton was significantly increased after LPS treatment, which suggests that LPS may induce the cytoskeleton reorganization and change the cardiomyocyte morphology.
authors.affiliation: Key Lab for Shock and Microcirculation Research, Department of Pathophysiology, Southern Medical University, Guangzhou 510515, PR China.
authors.forename: Liqun
authors.initials: L
authors.lastname: Wang
authors.forename: Tangting
authors.initials: T
authors.lastname: Chen
authors.forename: Xiang
authors.initials: X
authors.lastname: Zhou
authors.forename: Qiaobing
authors.initials: Q
authors.lastname: Huang
authors.forename: Chunhua
authors.initials: C
authors.lastname: Jin
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D003599:Cytoskeleton / Q000378:metabolism* / Q000648:ultrastructure*; D008070:Lipopolysaccharides / Q000378:metabolism*; D051379:Mice; D018625:Microscopy, Atomic Force; D032383:Myocytes, Cardiac / Q000187:drug effects* / Q000648:ultrastructure*
chemical_list: D008070:Lipopolysaccharides
keywords: Atomic force microscopy (AFM); Cardiomyocyte; Cytoskeleton; Lipopolysaccharide; Morphology