title: Characterization of sperm surface protein patterns of ejaculated and capacitated boar sperm, with the detection of ZP binding candidates.
abstract: Complementary molecules on the surface of both gametes are responsible for the interaction of sperm protein receptors with zona pellucida (ZP) saccharide structures, and many primary sperm receptors for ZP glycoproteins have been disclosed in various mammals. For our study, proteins were obtained from the surface of ejaculated and in vitro capacitated boar sperm. The isolated proteins were characterized by 1D- and 2D-electrophoretic protein profiles, and by glycoprotein staining. Our results show quantitative and qualitative differences in protein and glycoprotein patterns between ejaculated and capacitated sperm. Far-western blotting with ZP glycoproteins identified 17 interactions in the subproteome of the ejaculated sperm and 14 interactions in the subproteome of the capacitated sperm. High-molecular-mass proteins, coincident with binding to ZP, were sequence-identified. Angiotensin-converting enzyme (ACE), polycystic kidney disease receptor and egg jelly receptor (PKDREJ), and acrosin precursor were successfully identified. This is the first time PKDREJ has been identified on the surface of boar spermatozoa.
authors.affiliation: Laboratory of Reproductive Biology, Institute of Biotechnology, The Academy of Sciences of the Czech Republic, v.v.i., Vídeňská 1083, 142 20, Prague 4, Czech Republic; Department of Biochemistry, Faculty of Science, Charles University, Hlavova 8, 128 43, Prague 2, Czech Republic. Electronic address: michal_zigo_2000@yahoo.com.
authors.forename: Michal
authors.initials: M
authors.lastname: Zigo
authors.forename: Věra
authors.initials: V
authors.lastname: Jonáková
authors.forename: Miroslav
authors.initials: M
authors.lastname: Šulc
authors.forename: Pavla
authors.initials: P
authors.lastname: Maňásková-Postlerová
mesh_terms: D000818:Animals; D005260:Female; D008297:Male; D013058:Mass Spectrometry; D008562:Membrane Glycoproteins / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism*; D051379:Mice; D011485:Protein Binding; D020543:Proteome; D040901:Proteomics / Q000379:methods; D013075:Sperm Capacitation; D013094:Spermatozoa / Q000378:metabolism*; D034421:Sus scrofa; D015044:Zona Pellucida / Q000378:metabolism*
chemical_list: D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D020543:Proteome
keywords: PKDREJ protein; Sperm surface proteins; Zona pellucida-binding receptors