title: Distribution and development of P2Y1-purinoceptors in the mouse retina.
abstract: There is increasing evidence that ATP acts on purinergic receptors and mediates synaptic transmission in the retina. In a previous study, we raised the possibility that P2X-purinoceptors, presumably P2X(2)-purinoceptors in OFF-cholinergic amacrine cells, play a key role in the formation of OFF pathway-specific modulation. In this study, we examined whether the P2Y(1)-purinoceptors can function in cholinergic amacrine cells in the mouse retina since cholinergic amacrine cells in the rat retina express P2Y(1)-purinoceptors. P2Y(1)-purinoceptors were shown to be expressed in dendrites of both ON- and OFF-cholinergic amacrine cells in adults. At postnatal day 7, there was immunoreactivity for P2Y(1)-purinoceptors in the soma of cholinergic amacrine cells. At postnatal day 14, weak immunoreactivity for P2Y(1)-purinoceptors was detected in the dendrites but not in the soma of cholinergic amacrine cells. At postnatal day 21, strong immunoreactivity for P2Y(1)-purinoceptors was detected in dendrites of cholinergic amacrine cells. The expression pattern of P2Y(1)-purinoceptors was not affected by visual experience. We concluded that P2Y(1)-purinoceptors are not involved in the OFF-pathway-specific signal transmission in cholinergic amacrine cells of the mouse retina.
authors.affiliation: Department of Physiology, Nippon Medical School, Bunkyo-ku, Tokyo, 113-8602, Japan.
authors.forename: Rai
authors.initials: R
authors.lastname: Dilip
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Ishii
authors.forename: Hideki
authors.initials: H
authors.lastname: Imada
authors.forename: Yuko
authors.initials: Y
authors.lastname: Wada-Kiyama
authors.forename: Ryoiti
authors.initials: R
authors.lastname: Kiyama
authors.forename: Eiichi
authors.initials: E
authors.lastname: Miyachi
authors.forename: Makoto
authors.initials: M
authors.lastname: Kaneda
mesh_terms: D025042:Amacrine Cells / Q000378:metabolism; D000818:Animals; D059329:Cholinergic Neurons / Q000378:metabolism; D051379:Mice; D008822:Mice, Transgenic; D021381:Protein Transport; D051381:Rats; D058472:Receptors, Purinergic P2Y1 / Q000378:metabolism*; D012160:Retina / Q000378:metabolism*; D015398:Signal Transduction; D009435:Synaptic Transmission
chemical_list: D058472:Receptors, Purinergic P2Y1
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