title: Variation of NimC1 expression in Drosophila stocks and transgenic strains.
abstract: The NimC1 molecule has been described as a phagocytosis receptor, and is being used as a marker for professional phagocytes, the plasmatocytes, in Drosophila melanogaster. In studies including tumor-biology, developmental biology, and cell mediated immunity, monoclonal antibodies (P1a and P1b) to the NimC1 antigen are used. As we observed that these antibodies did not react with plasmatocytes of several strains and genetic combinations, a molecular analysis was performed on the structure of the nimC1 gene. In these strains we found 2 deletions and an insertion within the nimC1 gene, which may result in the production of a truncated NimC1 protein. The NimC1 positivity was regained by recombining the mutation with a wild-type allele or by using nimC1 mutant lines under heterozygous conditions. By means of these procedures or using the recombined stock, NimC1 can be used as a marker for phagocytic cells in the majority of the possible genetic backgrounds.
authors.affiliation: Institute of Genetics Biological Research Centre of the Hungarian Academy of Sciences; Szeged, Hungary.
authors.forename: Viktor
authors.initials: V
authors.lastname: Honti
authors.affiliation: Institute of Genetics Biological Research Centre of the Hungarian Academy of Sciences; Szeged, Hungary.
authors.forename: Gyöngyi
authors.initials: G
authors.lastname: Cinege
authors.affiliation: Institute of Genetics Biological Research Centre of the Hungarian Academy of Sciences; Szeged, Hungary.
authors.forename: Gábor
authors.initials: G
authors.lastname: Csordás
authors.affiliation: Institute of Genetics Biological Research Centre of the Hungarian Academy of Sciences; Szeged, Hungary.
authors.forename: Eva
authors.initials: E
authors.lastname: Kurucz
authors.affiliation: Institute of Genetics Biological Research Centre of the Hungarian Academy of Sciences; Szeged, Hungary.
authors.forename: János
authors.initials: J
authors.lastname: Zsámboki
authors.affiliation: Department of Molecular, Cell and Developmental Biology; University of California Los Angeles; Los Angeles, CA USA.
authors.forename: Cory J
authors.initials: CJ
authors.lastname: Evans
authors.affiliation: Department of Molecular, Cell and Developmental Biology; University of California Los Angeles; Los Angeles, CA USA.
authors.forename: Utpal
authors.initials: U
authors.lastname: Banerjee
authors.affiliation: Institute of Genetics Biological Research Centre of the Hungarian Academy of Sciences; Szeged, Hungary.
authors.forename: István
authors.initials: I
authors.lastname: Andó
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified / Q000235:genetics / Q000378:metabolism; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D004331:Drosophila melanogaster / Q000166:cytology / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D014644:Genetic Variation; D010586:Phagocytes / Q000378:metabolism*; D011971:Receptors, Immunologic / Q000235:genetics / Q000378:metabolism* / Q000502:physiology
chemical_list: D029721:Drosophila Proteins; C520113:NimC1 protein, Drosophila; D011971:Receptors, Immunologic
keywords: Drosophila; innate immunity; marker; phagocytosis receptor; plasmatocyte
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