title: [Sterilizing effect and residual bacteria of four different methods for sterilizing simple breathing vesicles].
abstract: OBJECTIVE
  To study the effects of 4 different methods for disinfection of simple breathing vesicles and microbial residue.
  
  
  METHODS
  The disinfection tests were divided into 4 groups: G1 group (43 cases) with 500 mg/L chlorine dioxide spray, G2 group (28 cases) with alcohol spray, G3 group (47 cases) with 50 mg/L trichloroisocyanuric acid (TCCA) immersion, and G4 group (46 cases) with 50 mg/L chlorine dioxide solution immersion. After 30 min of disinfection, each group was examined by bacterial culture and colony count. The residual bacteria were identified and typed.
  
  
  RESULTS
  The 4 methods showed significant differences in bacterial colony count (P<0.001). The rate of bacterial residue was 0% in G1 group, 53.6% in G2 group, 27.7% in G3 group, and 21.7% in G4 group, showing significant differences between the 4 groups (P<0.001). The residual bacteria included antibiotic-resistant common opportunistic pathogen such as Pseudomonas aeruginosa, Acinetobacter baumannii and Staphylococcus haemolytic.
  
  
  CONCLUSIONS
  Disinfection with 500 mg/L chlorine dioxide spray is the best for simple breathing vesicles. Prolonged immersion in TCCA may lead to the growth of drug-resistant pathogens in the breathing vesicles.
authors.affiliation: Department of Anesthesiology, Guangdong Province People's Hospital, Guangzhou, China. law982005@163.com
authors.forename: Aiwu
authors.initials: A
authors.lastname: Lu
authors.forename: Mei
authors.initials: M
authors.lastname: Wang
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Nie
authors.forename: Yuanbo
authors.initials: Y
authors.lastname: Guo
authors.forename: Chunxiao
authors.initials: C
authors.lastname: Wang
mesh_terms: D001419:Bacteria / Q000302:isolation & purification*; D015169:Colony Count, Microbial; D004203:Disinfection / Q000379:methods*; D024881:Drug Resistance, Bacterial; D012122:Ventilators, Mechanical