title: The distribution of ephrin-B1 and PNA-positive glycoconjugates is correlated with atypical melanoblast migration in Japanese Silky fowl embryos.
abstract: Melanoblasts are positively stimulated to migrate in the dorsolateral pathway of the avian embryo by ephrins, but are inhibited by PNA-binding glycoconjugates. We analyzed the potential role of these molecules in the Japanese Silky fowl, which displays intense internal pigmentation. The distribution of ephrin ligands was analyzed using Eph receptor-human Fc fusion proteins. Glycoconjugates were labeled using PNA-FITC. In Japanese Silky embryos, ventral areas, including the anterior- and posterior-half somites, expressed ephrin-B1 in a pattern that correlates with the atypical migratory pathways taken by Japanese Silky melanoblasts. White Leghorn embryos displayed little to no ephrin-Bs in the ventral paths. Conversely, PNA-binding barrier tissues, proposed to prevent melanoblasts from migrating ventrally in White Leghorn, are missing or have significant gaps in Japanese Silky embryos. Thus, studies of a naturally occurring pigmentation mutant confirm that a combination of cues regulates melanoblast migration in the chick embryo.
authors.affiliation: Laboratório de Biologia do Desenvolvimento, Departamento de Biologia Celular, Universidade Federal do Paraná, Centro Politécnico, Curitiba 81531-990, Paraná, Brazil. Electronic address: freitaspf@gmail.com.
authors.forename: Patrícia Franchi
authors.initials: PF
authors.lastname: Freitas
authors.affiliation: Faculdades Integradas do Brasil, Rua Konrad Adenauer, 442, Curitiba 82820-540, Paraná, Brazil.
authors.forename: Marisa Essenfelder
authors.initials: ME
authors.lastname: Borges
authors.affiliation: Laboratório de Biologia do Desenvolvimento, Departamento de Biologia Celular, Universidade Federal do Paraná, Centro Politécnico, Curitiba 81531-990, Paraná, Brazil.
authors.forename: Claudia Feijó
authors.initials: CF
authors.lastname: Ortolani-Machado
mesh_terms: D000818:Animals; D030161:Avian Proteins / Q000378:metabolism*; D002465:Cell Movement; D002642:Chick Embryo; D002645:Chickens / Q000033:anatomy & histology / Q000378:metabolism; D053595:Embryonic Stem Cells / Q000502:physiology*; D036387:Ephrin-B1 / Q000378:metabolism*; D006023:Glycoproteins / Q000378:metabolism*; D008544:Melanocytes / Q000502:physiology; D009432:Neural Crest / Q000166:cytology / Q000196:embryology; D009928:Organ Specificity; D019887:Peanut Agglutinin / Q000737:chemistry; D019170:Somites / Q000166:cytology / Q000378:metabolism
chemical_list: D030161:Avian Proteins; D036387:Ephrin-B1; D006023:Glycoproteins; D019887:Peanut Agglutinin
keywords: EphB receptors; Ephrin-B; Melanoblasts; Neural crest; PNA-binding; Silky fowl