title: Spectroscopic studies on the antioxidant activity of p-coumaric acid.
abstract: p-coumaric acid (4-hydroxycinnamic acid), a phenolic acid, is a hydroxyl derivative of cinnamic acid. It decreases low density lipoprotein (LDL) peroxidation and reduces the risk of stomach cancer. In vitro radical scavenging and antioxidant capacity of p-coumaric acid were clarified using different analytical methodologies such as total antioxidant activity determination by ferric thiocyanate, hydrogen peroxide scavenging, 1,1-diphenyl-2-picryl-hydrazyl free radical (DPPH) scavenging, 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) radical scavenging activity and superoxide anion radical scavenging, ferrous ions (Fe(2+)) chelating activity and ferric ions (Fe(3+)) reducing ability. p-Coumaric acid inhibited 71.2% lipid peroxidation of a linoleic acid emulsion at 45μg/mL concentration. On the other hand, butylated hydroxyanisole (BHA), butylated hydroxytoluene (BHT), α-tocopherol and ascorbic acid displayed 66.8%, 69.8%, 64.5% and 59.7% inhibition on the peroxidation of linoleic acid emulsion at the same concentration, respectively. In addition, p-coumaric acid had an effective DPPH scavenging, ABTS(+) scavenging, superoxide anion radical scavenging, hydrogen peroxide scavenging, ferric ions (Fe(3+)) reducing power and ferrous ions (Fe(2+)) chelating activities. Also, those various antioxidant activities were compared to BHA, BHT, α-tocopherol and ascorbic acid as references antioxidant compounds. These results suggested that p-coumaric acid can be used in the pharmacological and food industry because of these properties.
authors.affiliation: Trakya University, Faculty of Education, Edirne, Turkey. ismailk@trakya.edu.tr
authors.forename: Ismail
authors.initials: I
authors.lastname: Kiliç
authors.forename: Yeşim
authors.initials: Y
authors.lastname: Yeşiloğlu
mesh_terms: D000975:Antioxidants / Q000737:chemistry*; D001205:Ascorbic Acid / Q000737:chemistry; D052160:Benzothiazoles / Q000737:chemistry; D002083:Butylated Hydroxyanisole / Q000737:chemistry; D002084:Butylated Hydroxytoluene / Q000737:chemistry; D002614:Chelating Agents / Q000737:chemistry; D003373:Coumaric Acids / Q000737:chemistry*; D016166:Free Radical Scavengers / Q000737:chemistry; D006861:Hydrogen Peroxide / Q000737:chemistry; D007501:Iron / Q000737:chemistry; D010084:Oxidation-Reduction; D011422:Propionates; D012015:Reference Standards; D013057:Spectrum Analysis; D013451:Sulfonic Acids / Q000737:chemistry; D013481:Superoxides / Q000737:chemistry; D013861:Thiocyanates / Q000737:chemistry; D024502:alpha-Tocopherol / Q000737:chemistry
chemical_list: D000975:Antioxidants; D052160:Benzothiazoles; D002614:Chelating Agents; D003373:Coumaric Acids; D016166:Free Radical Scavengers; D011422:Propionates; D013451:Sulfonic Acids; D013861:Thiocyanates; D013481:Superoxides; D002084:Butylated Hydroxytoluene; D002083:Butylated Hydroxyanisole; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; C103724:ferric thiocyanate; D006861:Hydrogen Peroxide; D007501:Iron; D024502:alpha-Tocopherol; C495469:p-coumaric acid; D001205:Ascorbic Acid
keywords: 1,1-diphenyl-2-picrylhydrazyl; 2,2-azino-bis-(3-ethylbenzothiazoline-6-sulphonic acid); 3-(2-pyridyl)-5,6-bis(4-phenyl-sulphonic acid)-1,2,4-triazine; ABTS; Antioxidant activity; BHA; BHT; DPPH; EDTA; Ferrozine; Free radicals; Metal chelating; NADH; NBT; P-Coumaric acid; PMS; ROS; Reducing power; TCA; Tween-20; butylated hydroxyanisole; butylated hydroxytoluene; ethylenediaminetetra acetic acid; nicotinamide adenine dinucleotide; nitroblue tetrazolium; p-CA; p-coumaric acid; phenazine methosulfate; polyoxyethylenesorbitan monolaurate; reactive oxygen species; trichloroacetic acid