title: A proteomic approach to identify seminal plasma proteins in roosters (Gallus gallus domesticus).
abstract: Considering the interest in avian semen processing and storage, the objective of this study was to identify the domestic fowl seminal plasma proteins using two-dimensional gel electrophoresis (2-DE) and mass spectrometry MS/MS. For three times in a 4-month period, seminal plasma was obtained from semen collected from four local male chickens (Gallus gallus domesticus) and prepared for two-dimensional polyacrylamide gel electrophoresis. A total of 83 spots were detected across all gels and analyzed by MALDI-TOF/TOF. Among these spots, 17 have been successfully identified. The most intensely stained spots were recognized as serum albumin, ovotransferrin, alpha-enolase, fatty acid binding protein, thioredoxin, trypsin inhibitor CITI-1 and gallinacin-9. From these proteins, two are characteristic of avian seminal plasma, the ovotransferrin and gallinacin-9, and one is specific of the Gallus species, the chicken trypsin inhibitor CITI-1.
authors.affiliation: Department of Veterinary Sciences, Pisa University, Viale delle Piagge 2, Pisa, Italy. ghita@vet.unipi.it
authors.forename: Margherita
authors.initials: M
authors.lastname: Marzoni
authors.forename: Annelisse
authors.initials: A
authors.lastname: Castillo
authors.forename: Simona
authors.initials: S
authors.lastname: Sagona
authors.forename: Lorenzo
authors.initials: L
authors.lastname: Citti
authors.forename: Silvia
authors.initials: S
authors.lastname: Rocchiccioli
authors.forename: Isabella
authors.initials: I
authors.lastname: Romboli
authors.forename: Antonio
authors.initials: A
authors.lastname: Felicioli
mesh_terms: D000818:Animals; D002645:Chickens / Q000502:physiology*; D015180:Electrophoresis, Gel, Two-Dimensional / Q000662:veterinary; D008297:Male; D040901:Proteomics / Q000379:methods*; D012661:Semen / Q000737:chemistry*; D029589:Seminal Plasma Proteins / Q000032:analysis*; D053719:Tandem Mass Spectrometry / Q000662:veterinary
chemical_list: D029589:Seminal Plasma Proteins
keywords: Gallus gallus; Gel electrophoresis; Mass spectrometry; Proteomics; Rooster; Seminal plasma