title: Functional analysis of heterotrimeric G proteins in chloroplast development in Arabidopsis.
abstract: Functional analysis of G-proteins has been extensively carried out using their over-expressing lines and knockout mutants in plants. Since α subunit exists in an active or inactive form, overexpressing α subunit does not mean that G-protein signaling pathways are activated in the transgenic lines. Ectopic expression of the constitutively active form of the α subunit will magnify a role of G-protein signaling pathways in plant growth and development, and ultimately yield phenotypes. Here, we describe the method to study function of G-proteins in chloroplast development using the constitutively active form of the α subunit in Arabidopsis.
authors.affiliation: National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China.
authors.forename: Wenjuan
authors.initials: W
authors.lastname: Wu
authors.forename: Jirong
authors.initials: J
authors.lastname: Huang
mesh_terms: D017360:Arabidopsis / Q000254:growth & development* / Q000378:metabolism; D002736:Chloroplasts / Q000235:genetics / Q000378:metabolism*; D044385:GTP-Binding Protein alpha Subunits / Q000235:genetics; D044387:GTP-Binding Protein beta Subunits / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D020962:Heterotrimeric GTP-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction
chemical_list: D044385:GTP-Binding Protein alpha Subunits; D044387:GTP-Binding Protein beta Subunits; D020962:Heterotrimeric GTP-Binding Proteins