title: A representative of the dominant human colonic Firmicutes, Roseburia faecis M72/1, forms a novel bacteriocin-like substance.
abstract: During screening of human gut isolates in search of novel antimicrobials, the butyrate-producing strain Roseburia faecis M72/1 was found to produce an inhibitory substance active against Bacillus subtilis. Partial purification of the antimicrobial was achieved and activity found to be heat labile. Our findings suggest that R. faecis M72/1 produces a proteinaceous inhibitor whose production may be triggered by trypsin in the gastrointestinal tract.
authors.affiliation: Gut Health and Food Safety Programme, Institute of Food Research, Norwich Research Park, Colney, Norwich, NR4 7UA, UK.
authors.forename: Diane
authors.initials: D
authors.lastname: Hatziioanou
authors.forename: Melinda J
authors.initials: MJ
authors.lastname: Mayer
authors.forename: Sylvia H
authors.initials: SH
authors.lastname: Duncan
authors.forename: Harry J
authors.initials: HJ
authors.lastname: Flint
authors.forename: Arjan
authors.initials: A
authors.lastname: Narbad
mesh_terms: D001412:Bacillus subtilis / Q000187:drug effects*; D001430:Bacteriocins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D006094:Gram-Positive Bacteria / Q000302:isolation & purification / Q000378:metabolism*; D006801:Humans; D055550:Protein Stability; D013696:Temperature
chemical_list: D001430:Bacteriocins
keywords: Bacteriocin-like inhibitory substance (BLIS); Firmicutes anaerobe; Gut microbiota; Roseburia faecis; Trypsin