title: Detection of bovine group a rotavirus using rapid antigen detection kits, rt-PCR and next-generation DNA sequencing.
abstract: We investigated the sensitivity of human rotavirus rapid antigen detection (RAD) kits, RT-PCR and next-generation DNA sequencing (NGS) for detection of bovine group A rotavirus (RVA). The Dipstick 'Eiken' Rota (Dipstick) showed the highest sensitivity out of the seven RAD kits against all selected strains in limited dilution analyses, which was consistent with the results for equine rotavirus previously reported. RT-PCR had 10⁰-10³-fold higher sensitivity than the Dipstick. NGS using thirteen RT-PCR-negative fecal samples revealed that all samples yielded RVA reads and especially that two of them covered all 11 genome segments. Moreover, mapping reads to reference sequences allowed genotyping. The NGS would be sensitive and useful for analysis of less dependent on specific primers and screening of genotypes.
authors.affiliation: Ishikawa Nanbu Livestock Hygiene Service Center, Saida, Kanazawa, Ishikawa 920-3101, Japan.
authors.forename: Fujiko
authors.initials: F
authors.lastname: Minami-Fukuda
authors.forename: Makoto
authors.initials: M
authors.lastname: Nagai
authors.forename: Hikaru
authors.initials: H
authors.lastname: Takai
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Murakami
authors.forename: Tadashi
authors.initials: T
authors.lastname: Ozawa
authors.forename: Shinobu
authors.initials: S
authors.lastname: Tsuchiaka
authors.forename: Sachiko
authors.initials: S
authors.lastname: Okazaki
authors.forename: Yukie
authors.initials: Y
authors.lastname: Katayama
authors.forename: Mami
authors.initials: M
authors.lastname: Oba
authors.forename: Naomi
authors.initials: N
authors.lastname: Nishiura
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Sassa
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Omatsu
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Furuya
authors.forename: Satoshi
authors.initials: S
authors.lastname: Koyama
authors.forename: Junsuke
authors.initials: J
authors.lastname: Shirai
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Tsunemitsu
authors.forename: Yoshiki
authors.initials: Y
authors.lastname: Fujii
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Katayama
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Mizutani
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000302:isolation & purification; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis* / Q000821:virology*; D059014:High-Throughput Nucleotide Sequencing / Q000662:veterinary; D011933:Reagent Kits, Diagnostic / Q000662:veterinary* / Q000821:virology; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D012401:Rotavirus / Q000235:genetics* / Q000276:immunology*; D012400:Rotavirus Infections / Q000175:diagnosis / Q000662:veterinary*; D012680:Sensitivity and Specificity
chemical_list: D000956:Antigens, Viral; D011933:Reagent Kits, Diagnostic
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