title: Characterization of mineral phosphate solubilization traits from a barley rhizosphere soil functional metagenome.
abstract: Mineral phosphate solubilization (MPS) microorganisms are important for their provision of orthophosphate anions for plant growth promotion activity in soil. In this study, we applied a functional metagenomic approach to identify this trait directly from the microbiome in barley rhizosphere soil that had not received P fertilizer over a 15-year period. A fosmid system was used to clone the metagenome of which 18,000 clones (~666 Mb of DNA) was screened for MPS. Functional assays and High Performance Liquid Chromatography analysis recognized gluconic acid production and MPS activity in the range 24.8-77.1 mmol/L and 27.6-38.16 μg/mL, respectively, when screened in an Escherichia coli host (at frequency of one MPS-positive clone hit per 114 Mb DNA tested). The MPS clones (with average insert size of ~37 kb) were analysed by 454 Roche sequencing and annotated. A number of genes/operons with homology to Phosphorous (P) uptake, regulatory and solubilization mechanisms were identified, linking the MPS function to the uncultivated microbiome present in barley rhizosphere soil.
authors.affiliation: Department of Science and Health, Institute of Technology Carlow, Carlow, Ireland.
authors.forename: Sagar
authors.initials: S
authors.lastname: Chhabra
authors.forename: Dina
authors.initials: D
authors.lastname: Brazil
authors.forename: John
authors.initials: J
authors.lastname: Morrissey
authors.forename: James I
authors.initials: JI
authors.lastname: Burke
authors.forename: Fergal
authors.initials: F
authors.lastname: O'Gara
authors.forename: David
authors.initials: D
authors.lastname: N Dowling
mesh_terms: D002851:Chromatography, High Pressure Liquid; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005942:Gluconates / Q000378:metabolism; D001467:Hordeum / Q000378:metabolism / Q000382:microbiology*; D054892:Metagenome; D058977:Molecular Sequence Annotation; D009876:Operon; D010710:Phosphates / Q000378:metabolism*; D010802:Phylogeny; D058441:Rhizosphere; D017422:Sequence Analysis, DNA; D012987:Soil / Q000737:chemistry; D012988:Soil Microbiology; D012995:Solubility
chemical_list: D004269:DNA, Bacterial; D005942:Gluconates; D010710:Phosphates; D012987:Soil; C030691:gluconic acid
keywords: Glucose dehydrogenase; MPS; pyrroloquinoline quinone; soil metagenome.
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