title: Sequencing PCR-amplified DNA in lipoprotein and cardiovascular disease research.
abstract: The discovery of novel genetic variants and mutations in lipoprotein and cardiovascular disease research requires DNA sequencing. Large-scale genomics facilities will increasingly accomplish this with a combination of "next-generation" DNA sequencing methodologies. However, laboratories with limited access to these emerging technologies can still support focused genomic studies with the use of automated Sanger sequencing. Here, we describe two robust methods for medium-throughput DNA sequencing from PCR-amplified fragments of genomic DNA.
authors.affiliation: Pulmonary and Vascular Medicine Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, USA.
authors.forename: Victoria
authors.initials: V
authors.lastname: Youngblood
authors.forename: James G
authors.initials: JG
authors.lastname: Taylor
mesh_terms: D001483:Base Sequence; D035843:Biomedical Research; D002318:Cardiovascular Diseases / Q000235:genetics* / Q000473:pathology; D004247:DNA / Q000235:genetics; D020022:Genetic Predisposition to Disease; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D006801:Humans; D008074:Lipoproteins / Q000235:genetics* / Q000378:metabolism; D009154:Mutation; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*
chemical_list: D008074:Lipoproteins; D004247:DNA