title: Biomimetic composite scaffolds based mineralization of hydroxyapatite on electrospun calcium-containing poly(vinyl alcohol) nanofibers.
abstract: Nanocomposite materials consisting of polymer matrix and inorganic salts in the form of nanocrystals of hydroxyapatite (HA) are regarded as superior candidates for bone treatment. A biomimetic nanocomposite scaffold with HA formation on the electrospun poly(vinyl alcohol) (PVA) nanofibrous structure by employing a Ca-P alternate soaking method was developed in this work. The calcium-containing PVA nanofibers were prepared by adding calcium nitrate to the starting solution prior to electrospinning, and then mineralized by Ca-P treatment in incubation solution. With this rapid and effective procedure, a continuous biomimetic crystalline HA layer could be formed successfully without the need of a prior chemical modification of the substrate surface under very mild reaction conditions. Moreover, the HA formed with a relatively accelerated growth had a carbonated and poor crystalline structure, resembling biological apatite in the bone mineral. The introduction of calcium ions in nanofibers by electrospinning was a favorable approach to induce the deposition of calcium phosphate and improve the distribution, nucleation, and growth of crystalline HA layer on nanofibrous scaffolds. Bioactivity tests revealed that these mineralized PVA/HA composite scaffolds improved the biocompatibility. The porous polymer/HA composite scaffolds produced in the present study might have potential applications in bone tissue engineering.
authors.affiliation: State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing, 100029, PR China.
authors.forename: Wenkai
authors.initials: W
authors.lastname: Chang
authors.forename: Xueyan
authors.initials: X
authors.lastname: Mu
authors.forename: Xiaoqun
authors.initials: X
authors.lastname: Zhu
authors.forename: Guiping
authors.initials: G
authors.lastname: Ma
authors.forename: Chunguang
authors.initials: C
authors.lastname: Li
authors.forename: Fujian
authors.initials: F
authors.lastname: Xu
authors.forename: Jun
authors.initials: J
authors.lastname: Nie
mesh_terms: D040761:Biomimetic Materials / Q000494:pharmacology*; D002118:Calcium / Q000494:pharmacology; D002448:Cell Adhesion / Q000187:drug effects; D003432:Cross-Linking Reagents / Q000737:chemistry; D017886:Durapatite / Q000737:chemistry*; D004553:Electric Conductivity; D006801:Humans; D008903:Minerals / Q000737:chemistry*; D053761:Nanocomposites / Q000737:chemistry* / Q000648:ultrastructure; D057139:Nanofibers / Q000737:chemistry* / Q000648:ultrastructure; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects / Q000648:ultrastructure; D011142:Polyvinyl Alcohol / Q000494:pharmacology; D013052:Spectrometry, X-Ray Emission; D017550:Spectroscopy, Fourier Transform Infrared; D013818:Thermogravimetry; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*; D014783:Viscosity / Q000187:drug effects; D014961:X-Ray Diffraction
chemical_list: D003432:Cross-Linking Reagents; D008903:Minerals; D011142:Polyvinyl Alcohol; D017886:Durapatite; D002118:Calcium
keywords: Bone tissue engineering; Electrospinning; Hydroxyapatite; Mineralization; Scaffolds