title: Discrimination of Enterobacteriaceae and Non-fermenting Gram Negative Bacilli by MALDI-TOF Mass Spectrometry.
abstract: Discrimination of Enterobacteriaceae and Non-fermenting Gram Negative Bacilli by MALDI-TOF Mass Spectrometry Matrix assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) has proven to be an effective identification tool in medical microbiology. Discrimination to subspecies or serovar level has been found to be challenging using commercially available identification software. By forming our own reference database and using alternative analysis methods, we could reliably identify all implemented Enterobacteriaceae and non-fermenting gram negative bacilli by MALDI-TOF MS and even succeeded to distinguish Shigella sonnei from Escherichia coli (E. coli) and Salmonella enterica spp. enterica serovar Enteritidis from Salmonella enterica spp. enterica serovar Typhimurium. Furthermore, the method showed the ability to separate Enterohemorrhagic E. coli (EHEC) and Enteropathogenic E. coli (EPEC) from non-enteropathogenic E. coli.
authors.affiliation: Institute for Medical Microbiology and Epidemiology of Infectious Diseases, University of Leipzig, Leipzig, Germany.
authors.forename: Reiner
authors.initials: R
authors.lastname: Schaumann
authors.forename: Nicolas
authors.initials: N
authors.lastname: Knoop
authors.forename: Gelimer H
authors.initials: GH
authors.lastname: Genzel
authors.forename: Kevin
authors.initials: K
authors.lastname: Losensky
authors.forename: Christiane
authors.initials: C
authors.lastname: Rosenkranz
authors.forename: Catalina S
authors.initials: CS
authors.lastname: Stîngu
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Schellenberger
authors.forename: Arne C
authors.initials: AC
authors.lastname: Rodloff
authors.forename: Klaus
authors.initials: K
authors.lastname: Eschrich
keywords: E. coli; EHEC; EPEC; Enterobacteriaceae; MALDI-TOF MS; Salmonella spp; Shigella sonnei.
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