title: Biological pretreatment of corn stover with ligninolytic enzyme for high efficient enzymatic hydrolysis.
abstract: Aiming at increasing the efficiency of transferring corn stover into sugars, a biological pretreatment was developed and investigated in this study. The protocol was characterized by the pretreatment with crude ligninolytic enzymes from Phanerochete chrysosporium and Coridus versicolor to break the lignin structure in corn stover, followed by a washing procedure to eliminate the inhibition of ligninolytic enzyme on cellulase. By a 2 d-pretreatment, sugar yield from corn stover hydrolysis could be increased by 50.2% (up to 323 mg/g) compared with that of the control. X-ray diffractometry and FT-IR analysis revealed that biological pretreatment could partially remove the lignin of corn stover, and consequently enhance the enzymatic hydrolysis efficiency of cellulose and hemeicellulose. In addition, the amount of microbial inhibitors, such as acetic acid and furfural, were much lower in biological pretreatment than that in acid pretreatment. This study provided a promising pretreatment method for biotransformation of corn stovers.
authors.affiliation: Key Laboratory of Enzyme Engineering of Agricultural Microbiology, Ministry of Agriculture, College of Life Science, Henan Agricultural University, Zhengzhou 450002, China.
authors.forename: Feng-Qin
authors.initials: FQ
authors.lastname: Wang
authors.forename: Hui
authors.initials: H
authors.lastname: Xie
authors.forename: Wei
authors.initials: W
authors.lastname: Chen
authors.forename: En-Tao
authors.initials: ET
authors.lastname: Wang
authors.forename: Feng-Guang
authors.initials: FG
authors.lastname: Du
authors.forename: An-Dong
authors.initials: AD
authors.lastname: Song
mesh_terms: D002241:Carbohydrates / Q000096:biosynthesis; D002480:Cellulase / Q000378:metabolism*; D002482:Cellulose / Q000378:metabolism; D006868:Hydrolysis / Q000187:drug effects; D042845:Laccase / Q000378:metabolism; D008031:Lignin / Q000378:metabolism*; D013464:Sulfuric Acids / Q000494:pharmacology; D014866:Waste Products / Q000032:analysis*; D003313:Zea mays / Q000737:chemistry*
chemical_list: D002241:Carbohydrates; D013464:Sulfuric Acids; D014866:Waste Products; D002482:Cellulose; D008031:Lignin; D042845:Laccase; D002480:Cellulase; C033158:sulfuric acid; C109691:microcrystalline cellulose
keywords: Bio-pretreatment; Cellulase; Corn stover; Enzymatic hydrolysis; Ligninolytic enzyme