title: Role of surface layer collagen binding protein from indigenous Lactobacillus plantarum 91 in adhesion and its anti-adhesion potential against gut pathogen.
abstract: Human feacal isolates were ascertain as genus Lactobacillus using specific primer LbLMA1/R16-1 and further identified as Lactobacillus plantarum with species specific primers Lpl-3/Lpl-2. 25 L. plantarum strains were further assessed for hydrophobicity following the microbial adhesion to hydrocarbons (MATH) method and colonization potentials based on their adherence to immobilized human collagen type-1. Surface proteins were isolated from selected L. plantarum 91(Lp91) strain. The purified collagen binding protein (Cbp) protein was assessed for its anti-adhesion activity against enteric Escherichia coli 0157:H7 pathogen on immobilized collagen. Four L. plantarum strains displayed high degree of hydrophobicity and significant adhesion to collagen. A 72 kDa protein was purified which reduced 59.71% adhesion of E. coli 0157:H7 on immobilized collagen as compared to control well during adhesion assay. Cbp protein is the major influencing factor in inhibition of E. coli 0157:H7 adhesion with extracellular matrix (ECM) components. Hydrophobicity and adhesion potential are closely linked attributes precipitating in better colonization potential of the lactobacillus strains. Cbp is substantiated as a crucial surface protein contributing in adhesion of lactobacillus strains. The study can very well be the platform for commercialization of indigenous probiotic strain once their functional attributes are clinically explored.
authors.affiliation: National Dairy Research Institute, Karnal, Haryana, India. Electronic address: ashok.ndri@gmail.com.
authors.forename: Ashok Kumar
authors.initials: AK
authors.lastname: Yadav
authors.forename: Ashish
authors.initials: A
authors.lastname: Tyagi
authors.forename: Jai Kumar
authors.initials: JK
authors.lastname: Kaushik
authors.forename: Asha Chandola
authors.initials: AC
authors.lastname: Saklani
authors.forename: Sunita
authors.initials: S
authors.lastname: Grover
authors.forename: Virender Kumar
authors.initials: VK
authors.lastname: Batish
mesh_terms: D018829:Adhesins, Bacterial / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D000898:Antibiosis; D001422:Bacterial Adhesion / Q000187:drug effects*; D003094:Collagen / Q000378:metabolism*; D019453:Escherichia coli O157 / Q000187:drug effects* / Q000502:physiology; D005243:Feces / Q000382:microbiology; D041981:Gastrointestinal Tract / Q000382:microbiology; D006801:Humans; D048191:Lactiplantibacillus plantarum / Q000145:classification / Q000302:isolation & purification / Q000378:metabolism / Q000502:physiology*; D008565:Membrane Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D008970:Molecular Weight
chemical_list: D018829:Adhesins, Bacterial; D008565:Membrane Proteins; D003094:Collagen
keywords: Collagen binding protein; Human type-1 collagen; Indigenous; Lactobacillus plantarum