title: Non-viable Lactobacillus reuteri DSMZ 17648 (Pylopass™) as a new approach to Helicobacter pylori control in humans.
abstract: Prevalence of infections by Helicobacter pylori, a pathogen involved in a number of gastrointestinal diseases, remains high in developing countries. Management of infections by eradication is not always an option. Lactobacillus reuteri (L. reuteri) DSMZ17648 (Pylopass™/Lonza) specifically co-aggregates H. pylori in vitro and was shown to reduce ¹³C urea breath test in vivo. In this pilot study, we tried to replicate previous findings in an independent sample and to evaluate effects of spray-drying vs. freeze-drying of cultures. A single-blinded, placebo-controlled study was done in 22 H. pylori positive, asymptomatic adults. H. pylori levels were determined by ¹³C-urea-breath method after 14 days of supplementation, as well as after 6, 12, and 24 weeks follow-up. In the test group, but not in the placebo group, a significant reduction of H. pylori was observed. For the first time, spray-dried cells of L. reuteri DSMZ17648 have been used in a human study and results are in line with the first study results, supplementing with freeze-dried material. This is of special interest as spray-drying results in dead cell material, meaning that the effect of L. reuteri must be independent of its probiotic activity. These results confirm the potential of Pylopass™ as a novel way to reduce the load of H. pylori.
authors.affiliation: Experimental and Clinical Research Center, Charité Campus Berlin-Buch-CCB, Lindenberger Weg 80, Berlin 13125, Germany.
authors.forename: Heidrun
authors.initials: H
authors.lastname: Mehling
authors.forename: Andreas
authors.initials: A
authors.lastname: Busjahn
mesh_terms: D000328:Adult; D000368:Aged; D018592:Cross-Over Studies; D005260:Female; D005612:Freeze Drying; D016481:Helicobacter Infections / Q000628:therapy*; D016480:Helicobacter pylori / Q000187:drug effects*; D006801:Humans; D052200:Limosilactobacillus reuteri / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D010865:Pilot Projects; D019936:Probiotics / Q000008:administration & dosage*; D016037:Single-Blind Method; D016896:Treatment Outcome
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