title: A berberine-loaded electrospun poly-(epsilon-caprolactone) nanofibrous membrane with hemostatic potential and antimicrobial property for wound dressing.
abstract: In this study, a berberine-loaded electrospun poly-(epsilon-caprolactone) nanofibrous membrane (B-NFM) was formulated. The B-NFM was composed of uniform nanofibers with the average diameter of 190 +/- 53 nm. Whole blood assay revealed that the B-NFM had higher absorption capacity than gauze wound dressing (GWD) and non-drug-loaded nanofibrous membrane (NFM). Hemostatic studies showed that the B-NFM was more efficient at promoting blood clotting than GWD and NFM. B-NFM was also demonstrated to possess broad-spectrum antimicrobial activity. Biocompatibility studies indicated that B-NFM had good compatibility with skin fibroblast cells. These results suggested that the B-NFM can promote in vitro hemostasis to minimize wound bleeding, absorb biofluid to provide a favorable environment, inhibit on-site microbial agents to prevent wound infection, and support skin cell growth to facilitate wound healing. These data highly suggest a potential clinical use of B-NFM as wound dressing material.
authors.affiliation: Biomedical Engineering Program, University of South Dakota, 4800 North Career Avenue, Sioux Falls, South Dakota 57107, USA.
authors.forename: Jing
authors.initials: J
authors.lastname: Bao
authors.forename: Bo
authors.initials: B
authors.lastname: Yang
authors.forename: Yuyu
authors.initials: Y
authors.lastname: Sun
authors.forename: Youli
authors.initials: Y
authors.lastname: Zu
authors.forename: Ying
authors.initials: Y
authors.lastname: Deng
mesh_terms: D000900:Anti-Bacterial Agents / Q000008:administration & dosage* / Q000737:chemistry; D001458:Bandages / Q000382:microbiology*; D001599:Berberine / Q000008:administration & dosage* / Q000737:chemistry; D016063:Blood Loss, Surgical / Q000517:prevention & control*; D002478:Cells, Cultured; D004563:Electrochemistry / Q000379:methods; D005347:Fibroblasts / Q000187:drug effects; D006490:Hemostatics / Q000008:administration & dosage* / Q000737:chemistry; D006801:Humans; D008422:Materials Testing; D008567:Membranes, Artificial; D011091:Polyesters / Q000737:chemistry*; D012399:Rotation; D014945:Wound Healing
chemical_list: D000900:Anti-Bacterial Agents; D006490:Hemostatics; D008567:Membranes, Artificial; D011091:Polyesters; D001599:Berberine; C016240:polycaprolactone