title: Biochemical responses of Anticarsia gemmatalis (Lepidoptera: Noctuidae) in soybean cultivars sprayed with the protease inhibitor berenil.
abstract: The damage caused by Anticarsia gemmatalis motivates this study on the adaptive mechanisms of the insect to soybean. The lipoxygenase pathway produces and releases jasmonic acid, involved in the regulation of the plant defense genes, which encodes protease inhibitor (PI) production. Three soybean cultivars IAC-18, IAC-24, and Foscarin-31 were sprayed with water and berenil, a synthetic inhibitor, at 0.60 and 1.0% (w/v) and then infested with A. gemmatalis larvae. The lipoxygenase (LOX) activity increased in the leaves of Foscarin-31, IAC-18, and IAC-24 by 87, 81, and 78%, respectively, after 24 h of A. gemmatalis damage. IAC-18 revealed the lowest increase in PI when compared to the other cultivars. Protease, amidase, and esterase activities in soybean larvae dropped drastically after berenil application. PIs may be included in the control strategies of A. gemmatalis in soybean by lowering the digestive enzyme activity in the larval midgut, thus affecting insect growth and development.
authors.affiliation: Instituto de Biotecnologia Aplicada à Agropecuária (BIOAGRO), Universidade Federal de Viçosa, Viçosa, Minas Gerais (MG) 36570-000, Brazil.
authors.forename: Gilson P
authors.initials: GP
authors.lastname: Paixão
authors.forename: André L
authors.initials: AL
authors.lastname: Lourenção
authors.forename: Camila R
authors.initials: CR
authors.lastname: Silva
authors.forename: Eduardo G
authors.initials: EG
authors.lastname: Mendonça
authors.forename: Paulo L
authors.initials: PL
authors.lastname: Silva
authors.forename: Joel A
authors.initials: JA
authors.lastname: Oliveira
authors.forename: José C
authors.initials: JC
authors.lastname: Zanuncio
authors.forename: Maria Goreti A
authors.initials: MG
authors.lastname: Oliveira
mesh_terms: D000818:Animals; D004133:Diminazene / Q000031:analogs & derivatives* / Q000378:metabolism; D041981:Gastrointestinal Tract / Q000201:enzymology; D007300:Insect Control; D019476:Insect Proteins / Q000037:antagonists & inhibitors* / Q000378:metabolism; D008084:Lipoxygenase / Q000378:metabolism; D009036:Moths / Q000201:enzymology* / Q000254:growth & development / Q000378:metabolism; D010447:Peptide Hydrolases / Q000378:metabolism*; D010935:Plant Diseases / Q000469:parasitology*; D010940:Plant Proteins / Q000378:metabolism; D011480:Protease Inhibitors / Q000378:metabolism*; D013025:Glycine max / Q000201:enzymology / Q000469:parasitology*
chemical_list: D019476:Insect Proteins; D010940:Plant Proteins; D011480:Protease Inhibitors; D008084:Lipoxygenase; D010447:Peptide Hydrolases; C003915:diminazene aceturate; D004133:Diminazene