title: Ratchet nanofiltration of DNA.
abstract: The DNA nanofilter is a microfabricated electrophoretic separation device consisting of a periodic array of thin slits (circa 60 nm) separated by deeper wells (circa 320 nm). We demonstrate that this device can act as a tuneable, clog-free filter when operating in a low frequency, asymmetric field inversion mode. This filtration occurs by using asymmetric field inversion to achieve bi-directional migration of short (less than 1000 bp) DNA. Moreover, similar ratchet-type operation can improve separations when compared to a constant field separation in the same device. These modes of operation enhance the utility of the DNA nanofilter as a component of integrated lab-on-a-chip devices. The experimental data confirm theoretical predictions for the bidirectional transport of DNA in entropy-based separations.
authors.affiliation: Department of Chemical Engineering and Materials Science, University of Minnesota - Twin Cities, 421 Washington Ave SE, Minneapolis, MN 55455, USA.
authors.forename: Joel D P
authors.initials: JD
authors.lastname: Thomas
authors.forename: Mark N
authors.initials: MN
authors.lastname: Joswiak
authors.forename: Daniel W
authors.initials: DW
authors.lastname: Olson
authors.forename: Sung-Gyu
authors.initials: SG
authors.lastname: Park
authors.forename: Kevin D
authors.initials: KD
authors.lastname: Dorfman
mesh_terms: D004247:DNA / Q000737:chemistry / Q000302:isolation & purification*; D019277:Entropy; D005374:Filtration / Q000295:instrumentation / Q000379:methods*; D056656:Lab-On-A-Chip Devices; D008962:Models, Theoretical; D036103:Nanotechnology
chemical_list: D004247:DNA
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