title: Comprehensive Analysis of ETS Family Members in Melanoma by Fluorescence In Situ Hybridization Reveals Recurrent ETV1 Amplification.
abstract: E26 transformation-specific (ETS) transcription factors are known to be involved in gene aberrations in various malignancies including prostate cancer; however, their role in melanoma oncogenesis has yet to be fully explored. We have completed a comprehensive fluorescence in situ hybridization (FISH)-based screen for all 27 members of the ETS transcription factor family on two melanoma tissue microarrays, representing 223 melanomas, 10 nevi, and 5 normal skin tissues. None of the melanoma cases demonstrated ETS fusions; however, 6 of 114 (5.3%) melanomas were amplified for ETV1 using a break-apart FISH probe. For the six positive cases, locus-controlled FISH probes revealed that two of six cases were amplified for the ETV1 region, whereas four cases showed copy gains of the entire chromosome 7. The remaining 26 ETS family members showed no chromosomal aberrations by FISH. Quantitative polymerase chain reaction showed an average 3.4-fold (P value = .00218) increased expression of ETV1 in melanomas, including the FISH ETV1-amplified cases, when compared to other malignancies (prostate, breast, and bladder carcinomas). These data suggest that a subset of melanomas overexpresses ETV1 and amplification of ETV1 may be one mechanism for achieving high gene expression.
authors.affiliation: Michigan Center for Translational Pathology, University of Michigan Medical School, Ann Arbor, MI ; Department of Pathology, University of Michigan Medical School, Ann Arbor, MI ; The Comprehensive Cancer Center, University of Michigan Medical School, Ann Arbor, MI.
authors.forename: Rohit
authors.initials: R
authors.lastname: Mehra
authors.forename: Saravana M
authors.initials: SM
authors.lastname: Dhanasekaran
authors.forename: Nallasivam
authors.initials: N
authors.lastname: Palanisamy
authors.forename: Pankaj
authors.initials: P
authors.lastname: Vats
authors.forename: Xuhong
authors.initials: X
authors.lastname: Cao
authors.forename: Jung H
authors.initials: JH
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authors.forename: David Sl
authors.initials: DS
authors.lastname: Kim
authors.forename: Timothy
authors.initials: T
authors.lastname: Johnson
authors.forename: Douglas R
authors.initials: DR
authors.lastname: Fullen
authors.forename: Arul M
authors.initials: AM
authors.lastname: Chinnaiyan
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