title: Regulation of Helicoverpa armigera ecdysone receptor by miR-14 and its potential link to baculovirus infection.
abstract: Baculoviruses are well known for their ability to suppress the host development by altering its physiological and morphological changes. During its pathogenesis, the virus targets the host immune system, steroid hormones or apoptotic genes to evade anti-viral responses. Recent evidence indicate that viruses may use their own or host-encoded microRNAs (miRNA) to achieve this. In this study, utilizing microarray analysis, we found that the miRNA profile of Helicoverpa armigera larvae alters upon H. armigera single nucleopolyhedrovirus (HaSNPV) infection. Northern blot analyses revealed down-regulation of four miRNAs including the well-conserved and abundantly expressed miR-14. It was established that miR-14 positively regulates the H. armigera ecdysone receptor (Ha-EcR). Consistent with decreased levels of miR-14 following HaSNPV infection, the transcript levels of its target gene Ha-EcR declined around 72h post-infection. In vivo and in vitro studies revealed that in the presence of the synthetic miR-14 mimic the transcript levels of Ha-EcR significantly increased. The miRNA-target interaction was further confirmed by a GFP reporter assay. Further, application of the miRNA mimic led to reduced replication of the virus. These results suggest that, by down-regulating miR-14, HaSNPV may reduce the Ha-EcR transcript levels providing another classic example of virus-mediated manipulation of host physiology.
authors.affiliation: School of Biological Sciences, The University of Queensland, St. Lucia, QLD 4072, Australia.
authors.forename: Balachandran
authors.initials: B
authors.lastname: Jayachandran
authors.forename: Mazhar
authors.initials: M
authors.lastname: Hussain
authors.forename: Sassan
authors.initials: S
authors.lastname: Asgari
mesh_terms: D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D006790:Host-Parasite Interactions / Q000502:physiology*; D007814:Larva / Q000235:genetics / Q000821:virology; D035683:MicroRNAs / Q000235:genetics*; D008969:Molecular Sequence Data; D009036:Moths / Q000235:genetics* / Q000378:metabolism / Q000821:virology*; D017924:Nucleopolyhedroviruses / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis; D011987:Receptors, Steroid / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D035683:MicroRNAs; D011987:Receptors, Steroid; C055469:ecdysone receptor
keywords: Baculovirus; Ecdysone receptor; HaSNPV; MicroRNA