title: Electrochemical sensor for multiplex screening of genetically modified DNA: identification of biotech crops by logic-based biomolecular analysis.
abstract: Genetically modified (GM) technique, one of the modern biomolecular engineering technologies, has been deemed as profitable strategy to fight against global starvation. Yet rapid and reliable analytical method is deficient to evaluate the quality and potential risk of such resulting GM products. We herein present a biomolecular analytical system constructed with distinct biochemical activities to expedite the computational detection of genetically modified organisms (GMOs). The computational mechanism provides an alternative to the complex procedures commonly involved in the screening of GMOs. Given that the bioanalytical system is capable of processing promoter, coding and species genes, affirmative interpretations succeed to identify specified GM event in terms of both electrochemical and optical fashions. The biomolecular computational assay exhibits detection capability of genetically modified DNA below sub-nanomolar level and is found interference-free by abundant coexistence of non-GM DNA. This bioanalytical system, furthermore, sophisticates in array fashion operating multiplex screening against variable GM events. Such a biomolecular computational assay and biosensor holds great promise for rapid, cost-effective, and high-fidelity screening of GMO.
authors.affiliation: BioAnalytical Chemistry and Nanobiomedicine Laboratory, Department of Biochemical Science and Technology, National Taiwan University, Taipei 10617, Taiwan; Department of Chemistry, National Tsing Hua University, Hsinchu 30013, Taiwan.
authors.forename: Wei-Ching
authors.initials: WC
authors.lastname: Liao
authors.forename: Min-Chieh
authors.initials: MC
authors.lastname: Chuang
authors.forename: Ja-An Annie
authors.initials: JA
authors.lastname: Ho
mesh_terms: D001483:Base Sequence; D015374:Biosensing Techniques / Q000379:methods; D018556:Crops, Agricultural / Q000235:genetics*; D018744:DNA, Plant / Q000235:genetics*; D055664:Electrochemical Techniques / Q000379:methods*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D030821:Plants, Genetically Modified / Q000235:genetics*; D011401:Promoter Regions, Genetic
chemical_list: D018744:DNA, Plant
keywords: 3,3′,5,5′-tetramethylbenzidine; Av-GOx; Biomolecular assay; Biosensors; FITC; GMOs; Genetically modified organisms; Multiplex screening; Nucleic acids; TMB; fluorescein isothiocyanate; genetically modified organisms; glucose oxidase-labeled avidin; hydrogen peroxidase-labeled anti-FITC antibody; α-FITC-HRP