title: Hepatic cells derived from induced pluripotent stem cells of pigtail macaques support hepatitis C virus infection.
abstract: The narrow species tropism of hepatitis C virus (HCV) limits animal studies. We found that pigtail macaque (Macaca nemestrina) hepatic cells derived from induced pluripotent stem cells support the entire HCV life cycle, although infection efficiency was limited by defects in the HCV cell entry process. This block was overcome by either increasing occludin expression, complementing the cells with human CD81, or infecting them with a strain of HCV with less restricted requirements for CD81. Using this system, we can modify viral and host cell genetics to make pigtail macaques a suitable, clinically relevant model for the study of HCV infection.
authors.affiliation: Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, New York.
authors.forename: Marion
authors.initials: M
authors.lastname: Sourisseau
authors.forename: Orit
authors.initials: O
authors.lastname: Goldman
authors.forename: Wenqian
authors.initials: W
authors.lastname: He
authors.forename: Jennifer L
authors.initials: JL
authors.lastname: Gori
authors.forename: Hans-Peter
authors.initials: HP
authors.lastname: Kiem
authors.forename: Valerie
authors.initials: V
authors.lastname: Gouon-Evans
authors.forename: Matthew J
authors.initials: MJ
authors.lastname: Evans
mesh_terms: D000818:Animals; D002460:Cell Line; D002478:Cells, Cultured; D004195:Disease Models, Animal; D016174:Hepacivirus / Q000472:pathogenicity*; D006526:Hepatitis C / Q000473:pathology / Q000503:physiopathology / Q000821:virology*; D022781:Hepatocytes / Q000473:pathology / Q000821:virology*; D054884:Host-Pathogen Interactions / Q000235:genetics; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000473:pathology / Q000821:virology*; D008254:Macaca nemestrina; D062793:Occludin / Q000502:physiology; D060225:Tetraspanin 28 / Q000172:deficiency / Q000502:physiology; D053586:Virus Internalization; D014779:Virus Replication / Q000502:physiology
chemical_list: C554798:CD81 protein, human; D062793:Occludin; D060225:Tetraspanin 28
keywords: 2′C-methyl-adenosine; 2′CMA; Animal Model; GLuc; Gaussia luciferase; HCV; HCVcc; HCVpp; Hepatitis C Virus; Macaca nemestrina; Macaca nemestrina induced pluripotent stem cells; Macaca nemestrina–derived hepatocyte-like cells; Mn; MnHep; MniPSC; Monkey; OCLN; Replication; VSVGpp; cell culture–derived hepatitis C virus; hepatitis C virus; hepatitis C virus pseudoparticles; occludin; vesicular stomatitis virus glycoprotein
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