title: Attachment and proliferation of human osteoblast-like cells (MG-63) on laser-ablated titanium implant material.
abstract: Demand is increasing for shortening the long (3-6 months) osseointegration period to rehabilitate patients' damaged chewing apparatus in as short a time as possible. For dental implants, as for biomaterials in general, the bio- and osseointegration processes can be controlled at molecular and cellular levels by modification of the implant surface. One of the most promising of such surface modifications is laser ablation, as demonstrated by our previous results [46]. Commercially pure (CP4) sand-blasted, acid-etched titanium disks (Denti® System Ltd., Hungary) were irradiated with a KrF excimer laser (248 nm, fluence 0.4 J/cm(2), FWHM 18 ns, 2000 pulses), or with a Nd:YAG laser (532 nm, 1.3 J/cm(2), 10 ns, 200 pulses) then examined by SEM, AFM, and XPS. In vitro attachment (24 h) and proliferation (72 h) of MG-63 osteoblast cells were investigated via dimethylthiazol-diphenyl tetrazolium bromide (MTT), alamarBlue (AB) assays alkaline phosphatase quantification (ALP) and SEM. SEM and AFM revealed significant changes in morphology and roughness. XPS confirmed the presence of TiO2 on each sample; after Nd:YAG treatment a reduced state of Ti (Ti(3+)) was also observed. MTT, AB and ALP measurements detected an increase in the number of cells between the 24- and 72 hour observations; however, laser treatment did not affect cell attachment and proliferation significantly.
authors.affiliation: Department of Oral Biology and Experimental Dental Research, Faculty of Dentistry, University of Szeged, H-6720 Szeged, Hungary.
authors.forename: Ágnes
authors.initials: Á
authors.lastname: Györgyey
authors.forename: Krisztina
authors.initials: K
authors.lastname: Ungvári
authors.forename: Gabriella
authors.initials: G
authors.lastname: Kecskeméti
authors.forename: Judit
authors.initials: J
authors.lastname: Kopniczky
authors.forename: Béla
authors.initials: B
authors.lastname: Hopp
authors.forename: Albert
authors.initials: A
authors.lastname: Oszkó
authors.forename: István
authors.initials: I
authors.lastname: Pelsöczi
authors.forename: Zoltán
authors.initials: Z
authors.lastname: Rakonczay
authors.forename: Katalin
authors.initials: K
authors.lastname: Nagy
authors.forename: Kinga
authors.initials: K
authors.lastname: Turzó
mesh_terms: D000134:Acid Etching, Dental; D000469:Alkaline Phosphatase / Q000378:metabolism; D001672:Biocompatible Materials / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D015921:Dental Implants; D006801:Humans; D007834:Lasers; D018625:Microscopy, Atomic Force; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects / Q000201:enzymology / Q000648:ultrastructure; D056951:Photoelectron Spectroscopy; D014025:Titanium / Q000494:pharmacology*
chemical_list: D001672:Biocompatible Materials; D015921:Dental Implants; D014025:Titanium; D000469:Alkaline Phosphatase
keywords: Cell attachment and proliferation; Laser ablation; MG-63 osteoblasts; Surface modification; Titanium implant