title: [Analysis of full-length gene sequence of rabies vaccine virus aG strain].
abstract: To sequence and analyze the full-length gene sequence of rabies vaccine virus aG strain. The full-length gene sequence of aG strain was amplified by RT-PCR by 8 fragments,each PCR product was cloned into vector pGEM-T respectively, sequenced and assemblied; The 5' leader sequence was sequenced with method of 5' RACE. The homology between aG and other rabies vaccine virus was analyzed by using DNAstar and Mega4. 0 software. aG strain was 11 925nt(GenBank accession number: JN234411) in length and belonged to the genotype I . The Bioinformatics revealed that the homology showed disparation form different rabies vaccine virus. the full-length gene sequence of rabies vaccine virus aG strain provided a support for perfecting the standard for quality control of virus strains for production of rabies vaccine for human use in China.
authors.affiliation: National Institutes for Food and Drug Control, Beijing 100050,China.
authors.forename: Jia
authors.initials: J
authors.lastname: Li
authors.forename: Shou-Chun
authors.initials: SC
authors.lastname: Cao
authors.forename: Lei-Tai
authors.initials: LT
authors.lastname: Shi
authors.forename: Xiao-Hong
authors.initials: XH
authors.lastname: Wu
authors.forename: Jing-Hua
authors.initials: JH
authors.lastname: Liu
authors.forename: Yun-Peng
authors.initials: YP
authors.lastname: Wang
authors.forename: Jian-Rong
authors.initials: JR
authors.lastname: Tang
authors.forename: Yong-Xin
authors.initials: YX
authors.lastname: Yu
authors.forename: Guan-Mu
authors.initials: GM
authors.lastname: Dong
mesh_terms: D000595:Amino Acid Sequence; D000956:Antigens, Viral / Q000235:genetics / Q000276:immunology*; D001483:Base Sequence; D002681:China; D016679:Genome, Viral / Q000235:genetics*; D005838:Genotype; D006801:Humans; D008969:Molecular Sequence Data; D010802:Phylogeny; D011818:Rabies / Q000276:immunology / Q000517:prevention & control / Q000821:virology*; D011819:Rabies Vaccines / Q000276:immunology*; D011820:Rabies virus / Q000235:genetics* / Q000276:immunology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity
chemical_list: D000956:Antigens, Viral; D011819:Rabies Vaccines