title: RecA-mediated SOS response provides a geraniol tolerance in Escherichia coli.
abstract: Geraniol is an important industrial material and a potential candidate of advanced biofuels. One challenge of microbial geraniol production is the toxicity to hosts. However, the poor understanding on geraniol tolerance mechanism is an obstacle for developing geraniol tolerant host. This study genome-widely screened a shot-gun DNA library of Escherichia coli and found that recA is able to confer geraniol tolerance in E. coli. The recA knockout mutant was found extremely sensitive to geraniol. Based on our data, it was deciphered that recA provided tolerance through SOS response network responding to DNA damage caused by geraniol. RecA-mediated SOS response activates the homologous recombinational repair by RecB and RecN for corrective DNA maintenance. This protection mechanism suggests an effective strategy to combat geraniol toxicity in E. coli.
authors.affiliation: Division of Applied Life Science (BK21 Program), PMBBRC, Gyeongsang National University, 501 Jinju Dae-ro, Jinju 660-701, Republic of Korea.
authors.forename: Asad Ali
authors.initials: AA
authors.lastname: Shah
authors.forename: Chonglong
authors.initials: C
authors.lastname: Wang
authors.forename: Sang-Hwal
authors.initials: SH
authors.lastname: Yoon
authors.forename: Jae-Yean
authors.initials: JY
authors.lastname: Kim
authors.forename: Eui-Sung
authors.initials: ES
authors.lastname: Choi
authors.forename: Seon-Won
authors.initials: SW
authors.lastname: Kim
mesh_terms: D000080462:Acyclic Monoterpenes; D056804:Biofuels; D004260:DNA Repair; D004269:DNA, Bacterial / Q000235:genetics / Q000378:metabolism; D024881:Drug Resistance, Bacterial; D004926:Escherichia coli / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism*; D055786:Gene Knockout Techniques; D015723:Gene Library; D016680:Genome, Bacterial; D050296:Microbial Viability / Q000187:drug effects; D011938:Rec A Recombinases / Q000235:genetics / Q000378:metabolism*; D059767:Recombinational DNA Repair / Q000235:genetics; D013014:SOS Response, Genetics; D013729:Terpenes / Q000633:toxicity*
chemical_list: D000080462:Acyclic Monoterpenes; D056804:Biofuels; D004269:DNA, Bacterial; D029968:Escherichia coli Proteins; D013729:Terpenes; D011938:Rec A Recombinases; C007836:geraniol
keywords: DNA repair; Escherichia coli; Geraniol; RecA; SOS response; Stress tolerance