title: Thrombomodulin mediates the progression of epithelial ovarian cancer cells.
abstract: Thrombomodulin (TM), a natural anticoagulation factor, maintains circulation homeostasis in endothelial cells. TM has additional roles in modulating inflammation, thrombosis, and carcinogenesis. However, there is little information on the role of TM in the progression and metastasis of ovarian cancer. RNA silencing and cDNA expression vectors were used to manipulate target gene expression in ovarian cancer cells. Cell growth and migration were evaluated by an MTT assay, a wound-healing migration assay, a transwell migration assay, and a biosensor system. In this study, we found that TM silencing may enhance the growth rate of cells. The migratory ability of ovarian cancer cells was enhanced dramatically after TM silencing. TM overexpression in ovarian cells suppressed the proliferation and migration capability. Furthermore, we found that skov-3 cells treated with TM shRNA expressed high levels of fibronectin and vimentin and that the expression of these markers correlated positively with their migratory ability. Our results demonstrate that TM expression may regulate cell growth and migration in ovarian cancer cells. This finding suggests that TM may be a novel prognostic and therapeutic target for ovarian cancer.
authors.affiliation: Department of Obstetrics and Gynecology, Suzhou BenQ Medical Center, Nanjing Medical University, Suzhou, People's Republic of China.
authors.forename: Lu-Min
authors.initials: LM
authors.lastname: Chen
authors.forename: Weu
authors.initials: W
authors.lastname: Wang
authors.forename: Jen-Chih
authors.initials: JC
authors.lastname: Lee
authors.forename: Feng-Hsiang
authors.initials: FH
authors.lastname: Chiu
authors.forename: Chun-Te
authors.initials: CT
authors.lastname: Wu
authors.forename: Cheng-Jeng
authors.initials: CJ
authors.lastname: Tai
authors.forename: Chien-Kai
authors.initials: CK
authors.lastname: Wang
authors.forename: Chen-Jei
authors.initials: CJ
authors.lastname: Tai
authors.forename: Ming-Te
authors.initials: MT
authors.lastname: Huang
authors.forename: Yu-Jia
authors.initials: YJ
authors.lastname: Chang
mesh_terms: D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism; D015153:Blotting, Western; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics*; D049109:Cell Proliferation; D002470:Cell Survival / Q000235:genetics; D005260:Female; D005353:Fibronectins / Q000235:genetics / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D009375:Neoplasms, Glandular and Epithelial / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D010051:Ovarian Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D034622:RNA Interference; D020133:Reverse Transcriptase Polymerase Chain Reaction; D018180:Thrombomodulin / Q000235:genetics* / Q000378:metabolism; D014746:Vimentin / Q000235:genetics / Q000378:metabolism
chemical_list: D014408:Biomarkers, Tumor; D005353:Fibronectins; D018180:Thrombomodulin; D014746:Vimentin
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