title: Composition and properties of silver-containing calcium carbonate-calcium phosphate bone cement.
abstract: The introduction of silver, either in the liquid phase (as silver nitrate solution: Ag(L)) or in the solid phase (as silver phosphate salt: Ag(S)) of calcium carbonate-calcium phosphate (CaCO3-CaP) bone cement, its influence on the composition of the set cement (C-Ag(L) and C-Ag(S) cements with a Ca/Ag atomic ratio equal to 10.3) and its biological properties were investigated. The fine characterisation of the chemical setting of silver-doped and reference cements was performed using FTIR spectroscopy. We showed that the formation of apatite was enhanced from the first hours of maturation of C-Ag(L) cement in comparison with the reference cement, whereas a longer period of maturation (about 10 h) was required to observe this increase for C-Ag(S) cement, although in both cases, silver was present in the set cements mainly as silver phosphate. The role of silver nitrate on the setting chemical reaction is discussed and a chemical scheme is proposed. Antibacterial activity tests (S. aureus and S. epidermidis) and in vitro cytotoxicity tests (human bone marrow stromal cells (HBMSC)) showed that silver-loaded CaCO3-CaP cements had antibacterial properties (anti-adhesion and anti-biofilm formation) without a toxic effect on HBMSC cells, making C-Ag(S) cement a promising candidate for the prevention of bone implant-associated infections.
authors.affiliation: CIRIMAT, UPS-INPT-CNRS, ENSIACET, Université de Toulouse, 4, allée Emile Monso, BP 44362, 31030, Toulouse Cedex 4, France.
authors.forename: Sylvaine
authors.initials: S
authors.lastname: Jacquart
authors.forename: Robin
authors.initials: R
authors.lastname: Siadous
authors.forename: Christel
authors.initials: C
authors.lastname: Henocq-Pigasse
authors.forename: Reine
authors.initials: R
authors.lastname: Bareille
authors.forename: Christine
authors.initials: C
authors.lastname: Roques
authors.forename: Christian
authors.initials: C
authors.lastname: Rey
authors.forename: Christèle
authors.initials: C
authors.lastname: Combes
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry; D001031:Apatites / Q000737:chemistry; D018441:Biofilms; D001843:Bone Cements / Q000737:chemistry*; D001854:Bone Marrow Cells / Q000382:microbiology; D018786:Bone Substitutes; D002119:Calcium Carbonate / Q000737:chemistry*; D002130:Calcium Phosphates / Q000737:chemistry*; D006801:Humans; D008422:Materials Testing; D008826:Microbial Sensitivity Tests; D011208:Powders; D019736:Prostheses and Implants; D012834:Silver / Q000737:chemistry*; D013211:Staphylococcus aureus; D013212:Staphylococcus epidermidis; D017154:Stromal Cells / Q000382:microbiology
chemical_list: D000900:Anti-Bacterial Agents; D001031:Apatites; D001843:Bone Cements; D018786:Bone Substitutes; D002130:Calcium Phosphates; D011208:Powders; D012834:Silver; C020243:calcium phosphate; D002119:Calcium Carbonate
references.citation: Clin Infect Dis. 1998 Nov;27(5):1247-54
references.pmid: 9827278
references.citation: Biomaterials. 2004 Aug;25(18):4287-95
references.pmid: 15046919
references.citation: Clin Orthop Relat Res. 2011 Nov;469(11):3037-42
references.pmid: 21416206
references.citation: J Biomed Mater Res B Appl Biomater. 2009 May;89(2):342-352
references.pmid: 18823021
references.citation: J Biomed Mater Res. 2000 Dec 15;52(4):662-8
references.pmid: 11033548
references.citation: J Mater Sci Mater Med. 1998 Mar;9(3):129-34
references.pmid: 15348901
references.citation: Adv Pharmacol Sci. 2010;2010:910686
references.pmid: 21188244
references.citation: J Biomed Mater Res B Appl Biomater. 2010 Apr;93(1):51-8
references.pmid: 20024966
references.citation: In Vitro Cell Dev Biol Anim. 1993 Sep;29A(9):699-707
references.pmid: 8407713
references.citation: J Mater Sci Mater Med. 2002 Jan;13(1):125-31
references.pmid: 15348217
references.citation: Biomaterials. 2004 Aug;25(18):4383-91
references.pmid: 15046929
references.citation: J Biomed Mater Res B Appl Biomater. 2010 Nov;95(2):397-406
references.pmid: 20878924
references.citation: J Biomed Mater Res A. 2013 Sep;101(9):2456-64
references.pmid: 23349126
references.citation: Biomaterials. 2005 Dec;26(34):6880-6
references.pmid: 16005511
references.citation: J Arthroplasty. 2005 Dec;20(8):1055-9
references.pmid: 16376263
references.citation: Can J Microbiol. 2010 Apr;56(4):317-25
references.pmid: 20453898
references.citation: Acta Biomater. 2011 Nov;7(11):4064-70
references.pmid: 21763795
references.citation: J Immunol Methods. 1983 Dec 16;65(1-2):55-63
references.pmid: 6606682
references.citation: Biomaterials. 2006 Nov;27(32):5512-7
references.pmid: 16872671
references.citation: J Immunol Methods. 1983 Mar 11;58(1-2):225-37
references.pmid: 6339633
references.citation: J Biomed Mater Res A. 2006 Nov;79(2):318-28
references.pmid: 16817210
references.citation: J Biomed Mater Res B Appl Biomater. 2005 Aug;74(2):760-7
references.pmid: 15981171
references.citation: Postgrad Med J. 2000 Aug;76(898):479-83
references.pmid: 10908375
references.citation: Adv Drug Deliv Rev. 2012 Sep;64(12):1090-110
references.pmid: 22310160
references.citation: Adv Mater. 2013 Feb 25;25(8):1173-9
references.pmid: 23184492
references.citation: Eur Cell Mater. 2010 Jul 01;20:1-12
references.pmid: 20574942
references.citation: Am Fam Physician. 2001 Jun 15;63(12):2413-20
references.pmid: 11430456
references.citation: Clin Orthop Relat Res. 1979 Sep;(143):266-70
references.pmid: 509832