title: Dual functionalized graphene oxide serves as a carrier for delivering oligohistidine- and biotin-tagged biomolecules into cells.
abstract: A versatile method of dual chemical functionalization of graphene oxide (GO) with Tris-[nitrilotris(acetic acid)] (Tris-NTA) and biotin for cellular delivery of oligohistidine- and biotin-tagged biomolecules is reported. Orthogonally functionalized GO surfaces with Tris-NTA and biotin to obtain a dual-functionalized GO (DFGO) are prepared and characterized by various spectroscopic and microscopic techniques. Fluorescence microscopic images reveal that DFGO surfaces are capable of binding oligohistidine-tagged biomolecules/proteins and avidin/biotin-tagged biomolecules/proteins orthogonally. The DFGO nanoparticles are non-cytotoxic in nature and can deliver oligohistidine- and biotin-tagged biomolecules simultaneously into the cell.
authors.affiliation: Chemistry Division, CSIR Indian Institute of Chemical Biology, 4 Raja S. C. Mullick Road, Jadavpur, Kolkata, 700032, West Bengal, India.
authors.forename: Batakrishna
authors.initials: B
authors.lastname: Jana
authors.forename: Goutam
authors.initials: G
authors.lastname: Mondal
authors.forename: Atanu
authors.initials: A
authors.lastname: Biswas
authors.forename: Indrani
authors.initials: I
authors.lastname: Chakraborty
authors.forename: Abhijit
authors.initials: A
authors.lastname: Saha
authors.forename: Prashant
authors.initials: P
authors.lastname: Kurkute
authors.forename: Surajit
authors.initials: S
authors.lastname: Ghosh
mesh_terms: D001360:Avidin / Q000737:chemistry; D001710:Biotin / Q000737:chemistry*; D045744:Cell Line, Tumor; D004337:Drug Carriers / Q000138:chemical synthesis* / Q000494:pharmacology; D004847:Epithelial Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D005456:Fluorescent Dyes; D017930:Genes, Reporter; D006108:Graphite / Q000737:chemistry*; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006639:Histidine / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D018625:Microscopy, Atomic Force; D008856:Microscopy, Fluorescence; D053758:Nanoparticles / Q000737:chemistry*; D009571:Nitrilotriacetic Acid / Q000737:chemistry; D009842:Oligopeptides / Q000737:chemistry* / Q000378:metabolism; D010087:Oxides; D017550:Spectroscopy, Fourier Transform Infrared; D013194:Staining and Labeling / Q000379:methods; D014325:Tromethamine / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004337:Drug Carriers; D005456:Fluorescent Dyes; D009842:Oligopeptides; D010087:Oxides; D014325:Tromethamine; D001360:Avidin; D049452:Green Fluorescent Proteins; D006639:Histidine; D001710:Biotin; D006108:Graphite; D009571:Nitrilotriacetic Acid
keywords: Tris-NTA; biotin; cellular delivery; graphene oxide; surface functionalization