title: Screening anti-southern rice black-streaked dwarf virus drugs based on S7-1 gene expression in rice suspension cells.
abstract: Southern rice black-streaked dwarf virus (SRBSDV) is a rice pathogen that had an outbreak in southern China in 2010 and caused significant crop losses. Therefore, screening for effective antiviral drugs against SRBSDV is very important. This study used rice suspension cells infected with SRBSDV by polyethylene glycol-mediated uptake for screening antiviral drugs. SRBSDV P7-1, which is coded by the S7-1 gene, has an intrinsic ability to self-interact to form tubules that play an important role in viral infection. Therefore, relative expression level of the SRBSDV S7-1 gene in infected rice suspension cells was assayed by real-time quantitative polymerase chain reaction to evaluate the antiviral activities of various drugs. Dufulin displayed the highest inhibitory activity against SRBSDV S7-1 expression. In addition, changes in peroxidase (POD), polyphenol oxidase (PPO), and phenylalanine ammonia-lyase (PAL) activities were determined in inoculated and noninoculated cells. The results showed that both POD and PPO activities increased upon dufulin treatment. Furthermore, the validity of this approach was confirmed in an in vivo experiment in which dufulin was found to effectively inhibit SRBSDV.
authors.forename: Dandan
authors.initials: D
authors.lastname: Yu
authors.forename: Zhenchao
authors.initials: Z
authors.lastname: Wang
authors.forename: Jing
authors.initials: J
authors.lastname: Liu
authors.forename: Mingming
authors.initials: M
authors.lastname: Lv
authors.forename: Jiaju
authors.initials: J
authors.lastname: Liu
authors.forename: Xiangyang
authors.initials: X
authors.lastname: Li
authors.forename: Zhuo
authors.initials: Z
authors.lastname: Chen
authors.forename: Linghong
authors.initials: L
authors.lastname: Jin
authors.forename: Deyu
authors.initials: D
authors.lastname: Hu
authors.forename: Song
authors.initials: S
authors.lastname: Yang
authors.forename: Baoan
authors.initials: B
authors.lastname: Song
mesh_terms: D000998:Antiviral Agents / Q000494:pharmacology*; D052160:Benzothiazoles / Q000494:pharmacology; D018929:Cell Culture Techniques; D004353:Drug Evaluation, Preclinical; D015967:Gene Expression Regulation, Viral / Q000187:drug effects; D012275:Oryza / Q000821:virology*; D010935:Plant Diseases / Q000821:virology*; D010942:Plant Viruses / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D012087:Reoviridae / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D014764:Viral Proteins / Q000037:antagonists & inhibitors* / Q000235:genetics* / Q000378:metabolism
chemical_list: D000998:Antiviral Agents; D052160:Benzothiazoles; D014764:Viral Proteins; C576734:dufulin