title: A fluorescent chemodosimeter for Hg2+ based on a spirolactam ring-opening strategy and its application towards mercury determination in aqueous and cellular media.
abstract: A novel fluorescent chemosensor rhodamine B phenyl hydrazide (RBPH) for Hg(2+) was designed and synthesized. This probe is highly sensitive, selective, and irreversible for Hg(2+) and exhibits fluorescent response at 580 nm. RBPH also displayed detectable color change from colorless to pink upon treatment with Hg(2+). This property has been utilized as naked eye detection for Hg(2+) in various industrial samples. Fluorescence microscopic experiments demonstrated that this chemosensor is cell permeable and can be used for fluorescence imaging of Hg(2+) in cellular media. This probe can detect Hg(2+) with good linear relationships from 1 to 100 nM with r = 0.99983 and the limit of detection were found to be 0.019 nM with ± 0.91 % RSD at 10 nM concentrations.
authors.affiliation: Center for Nano and Material Sciences, Global Campus, Jain University, Jakkasandra (P), Kankapura (T), Bangalore, 560001, Karnataka, India.
authors.forename: Kempahanumakkagaari Suresh
authors.initials: KS
authors.lastname: Kumar
authors.forename: Thippeswamy
authors.initials: T
authors.lastname: Ramakrishnappa
authors.forename: R Geetha
authors.initials: RG
authors.lastname: Balakrishna
authors.forename: Mallingappagari
authors.initials: M
authors.lastname: Pandurangappa
mesh_terms: D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D006801:Humans; D006834:Hydrazines / Q000138:chemical synthesis / Q000737:chemistry*; D007769:Lactams / Q000737:chemistry*; D061986:MCF-7 Cells; D008628:Mercury / Q000032:analysis*; D012235:Rhodamines / Q000138:chemical synthesis / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013141:Spiro Compounds / Q000737:chemistry*; D014867:Water / Q000737:chemistry
chemical_list: D005456:Fluorescent Dyes; D006834:Hydrazines; D007769:Lactams; D012235:Rhodamines; D013141:Spiro Compounds; C543681:rhodamine B hydrazide; D014867:Water; D008628:Mercury
references.citation: Chem Rev. 1997 Aug 5;97(5):1515-1566
references.pmid: 11851458
references.citation: Org Biomol Chem. 2012 Oct 28;10(40):8076-81
references.pmid: 22932925
references.citation: J Am Chem Soc. 2005 Nov 16;127(45):15666-7
references.pmid: 16277484
references.citation: J Am Chem Soc. 2009 Feb 18;131(6):2040-1
references.pmid: 19159289
references.citation: Angew Chem Int Ed Engl. 2005 Jul 11;44(28):4405-7
references.pmid: 15945112
references.citation: Angew Chem Int Ed Engl. 2007;46(35):6658-61
references.pmid: 17665405
references.citation: Inorg Chem. 2007 Mar 5;46(5):1538-40
references.pmid: 17266305
references.citation: Talanta. 2008 Jun 30;76(1):9-14
references.pmid: 18585232
references.citation: Science. 2003 Aug 29;301(5637):1203
references.pmid: 12947190
references.citation: Chem Rev. 2008 Sep;108(9):3443-80
references.pmid: 18652512
references.citation: Inorg Chem. 2008 Aug 18;47(16):7190-201
references.pmid: 18637679
references.citation: Anal Chim Acta. 2010 Mar 17;663(1):85-90
references.pmid: 20172101
references.citation: Chem Rev. 2012 Mar 14;112(3):1910-56
references.pmid: 22040233
references.citation: Org Biomol Chem. 2011 Jun 21;9(12):4467-80
references.pmid: 21503366
references.citation: Org Lett. 2006 Aug 3;8(16):3413-6
references.pmid: 16869623
references.citation: J Environ Monit. 2009 Feb;11(2):330-5
references.pmid: 19212590
references.citation: Org Lett. 2009 Feb 19;11(4):859-61
references.pmid: 19166288
references.citation: Environ Sci Technol. 2007 Apr 15;41(8):2745-9
references.pmid: 17533833
references.citation: Org Lett. 2009 Jul 2;11(13):2740-3
references.pmid: 19507872
references.citation: Anal Chem. 2009 Dec 15;81(24):9993-10001
references.pmid: 19904913
references.citation: Chem Commun (Camb). 2008 Oct 21;(39):4759-61
references.pmid: 18830484
references.citation: J Am Chem Soc. 2003 Nov 26;125(47):14270-1
references.pmid: 14624563
references.citation: J Am Chem Soc. 2004 Mar 3;126(8):2272-3
references.pmid: 14982408
references.citation: J Fluoresc. 2008 Nov;18(6):1187-92
references.pmid: 18470600
references.citation: Inorg Chem. 2010 Feb 1;49(3):1206-10
references.pmid: 20041687
references.citation: Org Lett. 2010 Feb 5;12(3):476-9
references.pmid: 20067242
references.citation: J Am Chem Soc. 2006 Aug 9;128(31):9988-9
references.pmid: 16881608
references.citation: Angew Chem Int Ed Engl. 2008;47(42):8025-9
references.pmid: 18792904
references.citation: Chemistry. 2008;14(15):4719-24
references.pmid: 18386284
references.citation: J Am Chem Soc. 2007 Aug 29;129(34):10322-3
references.pmid: 17672463
references.citation: Chem Commun (Camb). 2011 Apr 21;47(15):4373-5
references.pmid: 21399827
references.citation: Analyst. 2010 Jul;135(7):1551-5
references.pmid: 20445891
references.citation: Talanta. 2009 Aug 15;79(3):775-9
references.pmid: 19576444
references.citation: Chem Rev. 2007 Feb;107(2):641-62
references.pmid: 17300143
references.citation: Chem Commun (Camb). 2012 Feb 4;48(11):1659-61
references.pmid: 22187718
references.citation: J Am Chem Soc. 2003 Mar 26;125(12):3418-9
references.pmid: 12643689
references.citation: Org Lett. 2006 Aug 17;8(17):3721-4
references.pmid: 16898801