title: Proper gibberellin localization in vascular tissue is required to regulate adventitious root development in tobacco.
abstract: Bioactive gibberellins (GAs) are involved in many developmental aspects of the life cycle of plants, acting either directly or through interaction with other hormones. Accumulating evidence suggests that GAs have an important effect on root growth; however, there is currently little information on the specific regulatory mechanism of GAs during adventitious root development. A study was conducted on tobacco (Nicotiana tabacum) plants for altered rates of biosynthesis, catabolism, and GA signalling constitutively or in specific tissues using a transgenic approach. In the present study, PtGA20ox, PtGA2ox1, and PtGAI were overexpressed under the control of the 35S promoter, vascular cambium-specific promoter (LMX5), or root meristem-specific promoter (TobRB7), respectively. Evidence is provided that the precise localization of bioactive GA in the stem but not in the roots is required to regulate adventitious root development in tobacco. High levels of GA negatively regulate the early initiation step of root formation through interactions with auxin, while a proper and mobile GA signal is required for the emergence and subsequent long-term elongation of established primordia. The results demonstrated that GAs have an inhibitory effect on adventitious root formation but a stimulatory effect on root elongation.
authors.affiliation: National Engineering Laboratory for Forest Tree Breeding, Key Laboratory for Genetics and Breeding of Forest Trees and Ornamental Plants of Ministry of Education, College of Biological Science and Technology, Beijing Forestry University, Beijing 100083, PR China.
authors.forename: Shihui
authors.initials: S
authors.lastname: Niu
authors.forename: Zhexin
authors.initials: Z
authors.lastname: Li
authors.forename: Huwei
authors.initials: H
authors.lastname: Yuan
authors.forename: Pan
authors.initials: P
authors.lastname: Fang
authors.forename: Xiaoyang
authors.initials: X
authors.lastname: Chen
authors.forename: Wei
authors.initials: W
authors.lastname: Li
mesh_terms: D005875:Gibberellins / Q000378:metabolism*; D007210:Indoleacetic Acids / Q000378:metabolism; D018517:Plant Roots / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism; D014026:Nicotiana / Q000235:genetics / Q000378:metabolism*
chemical_list: D005875:Gibberellins; D007210:Indoleacetic Acids
keywords: Adventitious root formation; Nicotiana tabacum; auxin–gibberellin interaction; gibberellin; root elongation; vascular tissue.
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