title: Crystallization and preliminary X-ray analysis of an alanine dehydrogenase from Bacillus megaterium WSH-002.
abstract: Alanine dehydrogenase (L-AlaDH) from Bacillus megaterium WSH-002 catalyses the NAD⁺-dependent interconversion of L-alanine and pyruvate. The enzyme was expressed in Escherichia coli BL21 (DE3) cells and purified with a His6 tag by Ni²⁺-chelating affinity chromatography for X-ray crystallographic analysis. Crystals were grown in a solution consisting of 0.1 M HEPES pH 8.0, 12%(w/v) polyethylene glycol 8000, 8%(v/v) ethylene glycol at a concentration of 15 mg ml⁻¹ purified protein. The crystal diffracted to 2.35 Å resolution and belonged to the trigonal space group R32, with unit-cell parameters a = b = 125.918, c = 144.698 Å.
authors.affiliation: Tianjin University of Science and Technology, Tianjin 300222, People's Republic of China.
authors.forename: Xiaoyun
authors.initials: X
authors.lastname: Lu
authors.forename: Qiufen
authors.initials: Q
authors.lastname: Yi
authors.forename: Guofang
authors.initials: G
authors.lastname: Zhang
authors.forename: Xianming
authors.initials: X
authors.lastname: Zhu
authors.forename: Honggang
authors.initials: H
authors.lastname: Zhou
authors.forename: Hui
authors.initials: H
authors.lastname: Dong
mesh_terms: D050788:Alanine Dehydrogenase / Q000737:chemistry* / Q000302:isolation & purification; D001410:Bacillus megaterium / Q000201:enzymology*; D001426:Bacterial Proteins / Q000737:chemistry* / Q000302:isolation & purification; D003460:Crystallization; D018360:Crystallography, X-Ray
chemical_list: D001426:Bacterial Proteins; D050788:Alanine Dehydrogenase
keywords: Bacillus megaterium; alanine dehydrogenase
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