title: Avoidance to wipe alcohol before venipuncture is not a source of spurious hemolysis.
abstract: BACKGROUND
  It is still uncertain whether or not avoidance to let disinfectant alcohol dry at the site of venipuncture is a source of spurious hemolysis when drawing venous blood.
  
  
  METHODS
  In a consecutive series of 52 outpatients referred for routine laboratory testing, venous blood was drawn by direct venipuncture with (odd group) or without (pair group) wiping 70% isopropyl alcohol at the site of venipuncture. A 3.5 mL evacuated tube with dot activator and gel separator was drawn from a vein of the upper limb, serum was immediately separated with standard centrifugation and tested for potassium, lactate dehydrogenase (LD), aspartate aminotransferase (AST) and hemolysis index (HI) on Roche Cobas.
  
  
  RESULTS
  No specimen was discarded for unsatisfactory venipuncture. No differences for age and gender were observed between groups. As regards the four parameters investigated, no significant differences could be observed between patients in whom blood was drawn with or without letting the alcohol dry. It is also noteworthy that no sample in both groups exceeded the conventional sample rejection threshold of cell-free hemoglobin.
  
  
  CONCLUSIONS
  The results of our prospective, randomized study attest that failure to wipe alcohol at the site of venipuncture should not be considered as a potential source of spurious hemolysis when drawing blood.
authors.affiliation: Department of Life and Reproduction Sciences, University of Verona, Verona, Italy.
authors.forename: Gian Luca
authors.initials: GL
authors.lastname: Salvagno
authors.forename: Elisa
authors.initials: E
authors.lastname: Danese
authors.forename: Gabriel
authors.initials: G
authors.lastname: Lima-Oliveira
authors.forename: Gian Cesare
authors.initials: GC
authors.lastname: Guidi
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Lippi
mesh_terms: D019840:2-Propanol / Q000008:administration & dosage*; D000368:Aged; D000594:Amino Acid Oxidoreductases / Q000097:blood; D004202:Disinfectants / Q000008:administration & dosage*; D005260:Female; D006461:Hemolysis; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000097:blood; D008297:Male; D008875:Middle Aged; D018962:Phlebotomy; D011188:Potassium / Q000097:blood; D011446:Prospective Studies
chemical_list: D004202:Disinfectants; D007770:L-Lactate Dehydrogenase; D000594:Amino Acid Oxidoreductases; C025745:aspartate dehydrogenase; D019840:2-Propanol; D011188:Potassium
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