title: Imaging of mitochondrial and nuclear Ca2+ dynamics in Arabidopsis roots.
abstract: Here we report a method for analyzing mitochondrial Ca(2+) dynamics in plant root cells by means of the newly generated cameleon probe 4mt-YC3.6. The use of plants expressing both nuclear- and mitochondrial-targeted cameleon, along with the resolution of confocal laser scanning microscopy (CLSM), allow simultaneous recordings of mitochondrial and nuclear Ca(2+) dynamics within the same cell.
authors.affiliation: Department of Biology, University of Padova, 35131 Padova, Italy.
authors.forename: Giovanna
authors.initials: G
authors.lastname: Loro
authors.forename: Alex
authors.initials: A
authors.lastname: Costa
mesh_terms: D017360:Arabidopsis / Q000166:cytology; D002118:Calcium / Q000032:analysis*; D002135:Calcium-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D002413:Cations, Divalent / Q000032:analysis; D002467:Cell Nucleus / Q000737:chemistry*; D003584:Cytological Techniques / Q000379:methods*; D005456:Fluorescent Dyes / Q000378:metabolism*; D018613:Microscopy, Confocal / Q000379:methods*; D008928:Mitochondria / Q000737:chemistry*; D018517:Plant Roots / Q000166:cytology; D013194:Staining and Labeling / Q000379:methods; D019076:Transgenes
chemical_list: D002135:Calcium-Binding Proteins; D002413:Cations, Divalent; D005456:Fluorescent Dyes; C495639:yellow cameleon; D002118:Calcium