title: Immunization with recombinant leucine aminopeptidase showed protection against Fasciola gigantica in mice.
abstract: Leucine aminopeptidase (LAP) is expressed in all stages of Fasciola gigantica and, hence, is considered as a potential vaccine candidate. In this study, we have tested a vaccine potential of LAP and the types of immune responses it elicited in vaccinated mice. Recombinant F. gigantica leucine aminopeptidase (rFgLAP) was expressed in Escherichia coli, BL21 (DE3). The imprinting control region mice subcutaneously immunized with 50 μg of rFgLAP combined with Freund's adjuvant (n = 10) exhibited a significant reduction in worm recoveries when compared with non-immunized and Freund's adjuvant controls at 60.8 and 64.3%, respectively, and both T helper (Th)1 and Th2 humoral immune responses were elicited in the hosts as reflected by the levels of IgG1 and IgG2a, with Th2 predominating. The levels of IgG1- and IgG2a-specific antibodies to rFgLAP were inversely and significantly correlated with the numbers of worm recoveries. The rFgLAP-vaccinated mice showed significantly reduced levels of serum glutamic oxaloacetic transaminase and serum glutamic pyruvic transaminase and liver damage. These indicated that rFgLAP has a potential as a vaccine candidate against F. gigantica, whose efficacy will be studied further in economic animals including cattle, sheep, and goat.
authors.affiliation: Department of Pathobiology, Faculty of Science, Mahidol University, Rama VI Rd, Ratchathewi, Bangkok, 10400, Thailand.
authors.forename: Narin
authors.initials: N
authors.lastname: Changklungmoa
authors.forename: Pornanan
authors.initials: P
authors.lastname: Kueakhai
authors.forename: Suda
authors.initials: S
authors.lastname: Riengrojpitak
authors.forename: Kulathida
authors.initials: K
authors.lastname: Chaithirayanon
authors.forename: Pannigan
authors.initials: P
authors.lastname: Chaichanasak
authors.forename: Narin
authors.initials: N
authors.lastname: Preyavichyapugdee
authors.forename: Pathanin
authors.initials: P
authors.lastname: Chantree
authors.forename: Veerawat
authors.initials: V
authors.lastname: Sansri
authors.forename: Tadashi
authors.initials: T
authors.lastname: Itagaki
authors.forename: Prasert
authors.initials: P
authors.lastname: Sobhon
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood; D004926:Escherichia coli; D005209:Fasciola / Q000145:classification*; D005211:Fascioliasis / Q000517:prevention & control*; D007074:Immunoglobulin G / Q000097:blood; D007931:Leucyl Aminopeptidase / Q000276:immunology*; D008099:Liver / Q000201:enzymology; D051379:Mice; D011994:Recombinant Proteins / Q000276:immunology*; D014612:Vaccines / Q000276:immunology*
chemical_list: D000909:Antibodies, Helminth; D007074:Immunoglobulin G; D011994:Recombinant Proteins; D014612:Vaccines; D007931:Leucyl Aminopeptidase
references.citation: Acta Trop. 2011 Jun;118(3):217-22
references.pmid: 21376699
references.citation: Nat Rev Immunol. 2007 Dec;7(12):975-87
references.pmid: 18007680
references.citation: Nat Rev Immunol. 2003 Dec;3(12):984-93
references.pmid: 14647480
references.citation: Vaccine. 2011 Nov 8;29(48):9057-63
references.pmid: 21939713
references.citation: J Comp Pathol. 1974 Apr;84(2):231-43
references.pmid: 4152761
references.citation: Parasitol Res. 2005 Sep;97(2):129-35
references.pmid: 15986249
references.citation: Mol Biochem Parasitol. 2010 Mar;170(1):1-6
references.pmid: 19931316
references.citation: Exp Parasitol. 2008 Jun;119(2):229-37
references.pmid: 18329021
references.citation: Vaccine. 2010 Aug 2;28(34):5551-7
references.pmid: 20600503
references.citation: J Biomed Biotechnol. 2010;2010:428593
references.pmid: 20150967
references.citation: Infect Immun. 1999 Apr;67(4):1954-61
references.pmid: 10085042
references.citation: Exp Mol Pathol. 2007 Apr;82(2):104-9
references.pmid: 17398281
references.citation: Int J Parasitol. 2004 May;34(6):703-14
references.pmid: 15111092
references.citation: Mol Biochem Parasitol. 2008 Mar;158(1):52-64
references.pmid: 18178266
references.citation: J Parasitol. 2004 Aug;90(4):876-9
references.pmid: 15357089
references.citation: Biochimie. 2010 Nov;92(11):1509-29
references.pmid: 20457213
references.citation: Exp Parasitol. 2012 Jul;131(3):283-91
references.pmid: 22543100
references.citation: Trends Biochem Sci. 2010 Jan;35(1):53-61
references.pmid: 19796954
references.citation: Vet Parasitol. 2000 Jul 24;91(1-2):153-8
references.pmid: 10889368
references.citation: Aust Vet J. 1995 Jul;72(7):275-6
references.pmid: 8534235
references.citation: Res Vet Sci. 1981 May;30(3):294-7
references.pmid: 6114523
references.citation: Nat Rev Immunol. 2011 Jun;11(6):375-88
references.pmid: 21610741
references.citation: Parasitol Res. 2010 May;106(6):1351-63
references.pmid: 20336319
references.citation: Parasitol Res. 2010 Mar;106(4):947-56
references.pmid: 20135148