title: Inhibition of Na+/H+ exchanger 1 by cariporide alleviates burn-induced multiple organ injury.
abstract: BACKGROUND
  Severe burns initiate an inflammatory response characterized by the upregulation of proinflammatory cytokine, which contributes to multiple organ injury. Na(+)/H(+) exchanger 1 (NHE1) plays a significant role in several inflammatory processes. This study was designed to investigate the role of NHE1 in burn-induced inflammation and multiple organ injury.
  
  
  METHODS
  Rats were subjected to a 30% total body surface area full-thickness burn. Cariporide was used to assess the function of NHE1 in burn-induced multiple organ injury by biochemical parameters, histologic changes, and inflammatory cytokine production.
  
  
  RESULTS
  We found that NHE1 expression was significantly increased after burn injury. Inhibition of NHE1 by cariporide attenuated burn-induced edema and tissue injury in heart, lung, kidney, and small intestine. Cariporide also inhibited plasma levels of tumor necrosis factor α, interleukin 6, and myeloperoxidase activity.
  
  
  CONCLUSIONS
  These results indicate that NHE1 inhibition prevents burn-induced multiple organ injury. The salutary effects afforded by NHE1 inhibition, at least in part, are mediated by attenuating systemic inflammatory response.
authors.affiliation: Department of Burns and Cutaneous Surgery, Xijing Hospital, The Fourth Military Medical University, Xi'an, Shaanxi, China.
authors.forename: Xuekang
authors.initials: X
authors.lastname: Yang
authors.forename: Hua
authors.initials: H
authors.lastname: Bai
authors.forename: Weixia
authors.initials: W
authors.lastname: Cai
authors.forename: Jiaqi
authors.initials: J
authors.lastname: Liu
authors.forename: Yunchuan
authors.initials: Y
authors.lastname: Wang
authors.forename: Yuqiao
authors.initials: Y
authors.lastname: Xu
authors.forename: Jun
authors.initials: J
authors.lastname: Li
authors.forename: Qin
authors.initials: Q
authors.lastname: Zhou
authors.forename: Juntao
authors.initials: J
authors.lastname: Han
authors.forename: Xiongxiang
authors.initials: X
authors.lastname: Zhu
authors.forename: Maolong
authors.initials: M
authors.lastname: Dong
authors.forename: Dahai
authors.initials: D
authors.lastname: Hu
mesh_terms: D000818:Animals; D000889:Anti-Arrhythmia Agents / Q000494:pharmacology; D002056:Burns / Q000150:complications / Q000188:drug therapy*; D004195:Disease Models, Animal; D015870:Gene Expression / Q000187:drug effects; D006146:Guanidines / Q000494:pharmacology*; D007422:Intestines / Q000187:drug effects / Q000502:physiology; D007668:Kidney / Q000187:drug effects / Q000502:physiology; D008168:Lung / Q000187:drug effects / Q000502:physiology; D008297:Male; D009102:Multiple Organ Failure / Q000188:drug therapy* / Q000209:etiology; D009200:Myocardial Contraction / Q000187:drug effects / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D000074082:Sodium-Hydrogen Exchanger 1; D017923:Sodium-Hydrogen Exchangers / Q000037:antagonists & inhibitors* / Q000235:genetics; D013450:Sulfones / Q000494:pharmacology*
chemical_list: D000889:Anti-Arrhythmia Agents; D006146:Guanidines; C452850:Slc9a1 protein, rat; D000074082:Sodium-Hydrogen Exchanger 1; D017923:Sodium-Hydrogen Exchangers; D013450:Sulfones; C093373:cariporide
keywords: Burns; Inflammation; Multiple organ injury; Na(+)/H(+) exchanger 1