title: Subtype identification in acutely dissociated rat nodose ganglion neurons based on morphologic parameters.
abstract: Nodose ganglia are composed of A-, Ah- and C-type neurons. Despite their important roles in regulating visceral afferent function, including cardiovascular, pulmonary, and gastrointestinal homeostasis, information about subtype-specific expression, molecular identity, and function of individual ion transporting proteins is scarce. Although experiments utilizing the sliced ganglion preparation have provided valuable insights into the electrophysiological properties of nodose ganglion neuron subtypes, detailed characterization of their electrical phenotypes will require measurements in isolated cells. One major unresolved problem, however, is the difficulty to unambiguously identify the subtype of isolated nodose ganglion neurons without current-clamp recording, because the magnitude of conduction velocity in the corresponding afferent fiber, a reliable marker to discriminate subtypes in situ, can no longer be determined. Here, we present data supporting the notion that application of an algorithm regarding to microscopic structural characteristics, such as neuron shape evaluated by the ratio between shortest and longest axis, neuron surface characteristics, like membrane roughness, and axon attachment, enables specific and sensitive subtype identification of acutely dissociated rat nodose ganglion neurons, by which the accuracy of identification is further validated by electrophysiological markers and overall positive predictive rates is 89.26% (90.04%, 76.47%, and 98.21% for A-, Ah, and C-type, respectively). This approach should aid in gaining insight into the molecular correlates underlying phenotypic heterogeneity of nodose ganglia. Additionally, several critical points that help for neuron identification and afferent conduction calibration are also discussed.
authors.affiliation: Department of Pharmacology, Harbin Medical University, Harbin, China.
authors.forename: Xiao-Long
authors.initials: XL
authors.lastname: Lu
authors.forename: Wen-Xiao
authors.initials: WX
authors.lastname: Xu
authors.forename: Zhen-Yu
authors.initials: ZY
authors.lastname: Yan
authors.forename: Zhao
authors.initials: Z
authors.lastname: Qian
authors.forename: Bing
authors.initials: B
authors.lastname: Xu
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.forename: Li-Min
authors.initials: LM
authors.lastname: Han
authors.forename: Rui-Chen
authors.initials: RC
authors.lastname: Gao
authors.forename: Jun-Nan
authors.initials: JN
authors.lastname: Li
authors.forename: Mei
authors.initials: M
authors.lastname: Yuan
authors.forename: Chong-Bao
authors.initials: CB
authors.lastname: Zhao
authors.forename: Guo-Fen
authors.initials: GF
authors.lastname: Qiao
authors.forename: Bai-Yan
authors.initials: BY
authors.lastname: Li
mesh_terms: D000200:Action Potentials / Q000502:physiology; D000818:Animals; D000831:Animals, Newborn; D005260:Female; D008297:Male; D009413:Nerve Fibers, Myelinated / Q000648:ultrastructure; D036421:Nerve Fibers, Unmyelinated / Q000648:ultrastructure; D009474:Neurons / Q000166:cytology / Q000502:physiology*; D009620:Nodose Ganglion / Q000166:cytology*; D018408:Patch-Clamp Techniques; D051381:Rats; D017207:Rats, Sprague-Dawley
keywords: action potential; conduction velocity; visceral sensory neuron; visualization; whole-cell patch
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