title: ß-Phenylethylamine as a novel nutrient treatment to reduce bacterial contamination due to Escherichia coli O157:H7 on beef meat.
abstract: Bacterial infection by Escherichia coli O157:H7 through the consumption of beef meat or meat products is an ongoing problem, in part because bacteria develop resistances towards chemicals aimed at killing them. In an approach that uses bacterial nutrients to manipulate bacteria into behaviors or cellular phenotypes less harmful to humans, we screened a library of 95 carbon and 95 nitrogen sources for their effect on E. coli growth, cell division, and biofilm formation. In the initial screening experiment using the Phenotype MicroArray(TM) technology from BioLog (Hayward, CA), we narrowed the 190 starting nutrients down to eight which were consecutively tested as supplements in liquid beef broth medium. Acetoacetic acid (AAA) and ß-phenylethylamine (PEA) performed best in this experiment. On beef meat pieces, PEA reduced the bacterial cell count by 90% after incubation of the PEA treated and E. coli contaminated meat pieces at 10°C for one week.
authors.affiliation: Department of Veterinary and Microbiological Sciences, North Dakota State University, Fargo, ND 58108, United States.
authors.forename: Ty
authors.initials: T
authors.lastname: Lynnes
authors.forename: S M
authors.initials: SM
authors.lastname: Horne
authors.forename: B M
authors.initials: BM
authors.lastname: Prüß
mesh_terms: D000090:Acetoacetates / Q000008:administration & dosage; D000818:Animals; D001216:Asparagine / Q000008:administration & dosage; D002210:Caprylates / Q000008:administration & dosage; D002417:Cattle; D015169:Colony Count, Microbial; D003257:Consumer Product Safety; D019453:Escherichia coli O157 / Q000187:drug effects* / Q000254:growth & development; D005506:Food Contamination / Q000517:prevention & control*; D005511:Food Handling; D005516:Food Microbiology; D006595:Hexosamines / Q000008:administration & dosage; D020128:Inhibitory Concentration 50; D008460:Meat / Q000382:microbiology*; D010627:Phenethylamines / Q000008:administration & dosage*; D013941:Thymine / Q000008:administration & dosage
chemical_list: D000090:Acetoacetates; D002210:Caprylates; D006595:Hexosamines; D010627:Phenethylamines; C008322:mannosamine; C029261:phenethylamine; C016635:acetoacetic acid; D001216:Asparagine; D013941:Thymine
keywords: Beef meat; E. coli O157:H7; Post-harvest treatment; ß-Phenylethylamine