title: Genetic diversity of the honeybee Apis cerana in Yunnan, China, based on mitochondrial DNA.
abstract: DNA sequence diversity in the tRNA(leu)-COII portion of the mitochondrial genome was investigated in samples of Apis cerana from Yunnan, China. A fragment of about 480 bp in tRNA(leu)-COII, including a noncoding area and part of COII, was sequenced. The noncoding area was 97-98 bp; 8 haplotypes were found, among which 5 had been reported previously, while 3 were new. The mean diversity of haplotypes was 0.752 ± 0.030 (0.378-0.698), and nucleotide diversity was 0.01073 ± 0.00087 (0.00412-0.01123). A neighbor-joining tree was constructed based on 73 sequences of noncoding intergenic regions in the mtDNA of A. cerana from China and other Asian countries; all haplotypes found in China fell into the mainland Asian group. This result does not support the hypothesis that A. cerana indica occurs in southern Yunnan, which was concluded in a recent report based on morphological variation.
authors.affiliation: College of Animal Science and Technology, Yangzhou University, Yangzhou, China.
authors.forename: L
authors.initials: L
authors.lastname: Yin
authors.forename: T
authors.initials: T
authors.lastname: Ji
mesh_terms: D000818:Animals; D001516:Bees / Q000235:genetics*; D002681:China; D004272:DNA, Mitochondrial; D019143:Evolution, Molecular; D014644:Genetic Variation; D054629:Genome, Mitochondrial; D006239:Haplotypes; D008928:Mitochondria / Q000235:genetics*; D010802:Phylogeny; D012356:RNA, Transfer, Leu / Q000235:genetics*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
chemical_list: D004272:DNA, Mitochondrial; D012356:RNA, Transfer, Leu