title: Differential migration of passenger leukocytes and rapid deletion of naive alloreactive CD8 T cells after mouse liver transplantation.
abstract: Donor passenger leukocytes (PLs) from transplanted livers migrate to recipient lymphoid tissues, where they are thought to induce the deletion of donor-specific T cells and tolerance. Difficulties in tracking alloreactive T cells and PLs in rats and in performing this complex surgery in mice have limited progress in identifying the contribution of PL subsets and sites and the kinetics of T cell deletion. Here we developed a mouse liver transplant model in which PLs, recipient cells, and a reporter population of transgenic CD8 T cells specific for the graft could be easily distinguished and quantified in allografts and recipient organs by flow cytometry. All PL subsets circulated rapidly via the blood as soon as 1.5 hours after transplantation. By 24 hours, PLs were distributed differently in the lymph nodes and spleen, whereas donor natural killer and natural killer T cells remained in the liver and blood. Reporter T cells were activated in both liver and lymphoid tissues, but their numbers dramatically decreased within the first 48 hours. These results provide the first unequivocal demonstration of the differential recirculation of liver PL subsets after transplantation, and show that alloreactive CD8 T cells are deleted more rapidly than initially reported. This model will be useful for dissecting early events leading to the spontaneous acceptance of liver transplants.
authors.affiliation: Liver Immunology Group, Centenary Institute, Newtown, Australia; A. W. Morrow Gastroenterology and Liver Centre, Royal Prince Alfred Hospital, University of Sydney, Sydney, Australia.
authors.forename: Szun S
authors.initials: SS
authors.lastname: Tay
authors.forename: Bo
authors.initials: B
authors.lastname: Lu
authors.forename: Fred
authors.initials: F
authors.lastname: Sierro
authors.forename: Volker
authors.initials: V
authors.lastname: Benseler
authors.forename: Claire M
authors.initials: CM
authors.lastname: McGuffog
authors.forename: G Alex
authors.initials: GA
authors.lastname: Bishop
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Cowan
authors.forename: Geoffrey W
authors.initials: GW
authors.lastname: McCaughan
authors.forename: Karen M
authors.initials: KM
authors.lastname: Dwyer
authors.forename: David G
authors.initials: DG
authors.lastname: Bowen
authors.forename: Patrick
authors.initials: P
authors.lastname: Bertolino
mesh_terms: D064591:Allografts; D000818:Animals; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D002465:Cell Movement; D007962:Leukocytes / Q000166:cytology / Q000502:physiology*; D016031:Liver Transplantation; D008213:Lymphocyte Activation; D008297:Male; D008810:Mice, Inbred C57BL; D055611:Natural Killer T-Cells / Q000502:physiology; D014019:Tissue Donors