title: Diversity and antimicrobial susceptibility of Salmonella enterica serovars isolated from pig farms in Ibadan, Nigeria.
abstract: Animals including food animals play a significant role in the epidemiology of Salmonella enterica. The control requires identification of sources and institution of targeted interventions. This study investigates the diversity of S. enterica serovars, antimicrobial susceptibility, and occurrence of plasmid-mediated quinolone resistance (PMQR) genes in pigs in Ibadan, Nigeria. Pooled fresh pen floor fecal samples of pigs collected from 31 pig farms were cultured; the Salmonella isolates were serotyped and their antimicrobial susceptibility was determined. PMQR genes were screened by polymerase chain reaction. The 229 Salmonella isolates were made of 50 serovars predominated by rare serovars Salmonella Give (n = 36; 15.7 %), Salmonella Brancaster (n = 17; 7.4 %), Salmonella Colindale (n = 15; 6.6 %), Salmonella Elisaberthville (n = 13; 5.7 %), Salmonella Hillingdon (n = 13; 5.7 %), and Salmonella Kingston (n = 13; 5.7 %). The most widely distributed serovars among the farms were Salmonella Give (six farms) and Salmonella Elisaberthville (six farms). Resistance to chloramphenicol, sulfonamides, nalidixic acid, streptomycin, and tetracycline ranged from 11.6 % (n = 26) to 22.8 % (n = 51). Resistance ciprofloxacin and gentamicin was low (n = 2; 0.9 %). Multiply resistant isolates included Salmonella Kentucky, the most resistant serovar. qnrB19 was found in two isolates of Salmonella Corvallis and one isolate of Salmonella Larochelle, respectively, while qnrS1 was found in two isolates of Salmonella Derby. Other PMQR genes were not detected. Pigs constitute an important source of diverse Salmonella serovars in Ibadan. The isolates were more resistant to old antimicrobials with some multiple resistant. Control measures and regulation of antimicrobials are warranted.
authors.affiliation: Department of Microbiology, University of Ibadan, Ibadan, Nigeria, fashaekayode@yahoo.com.
authors.forename: Kayode
authors.initials: K
authors.lastname: Fashae
authors.forename: Rene S
authors.initials: RS
authors.lastname: Hendriksen
mesh_terms: D000818:Animals; D000829:Animals, Domestic; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D004269:DNA, Bacterial / Q000235:genetics; D005243:Feces / Q000382:microbiology; D005798:Genes, Bacterial; D008826:Microbial Sensitivity Tests; D009549:Nigeria; D010957:Plasmids; D019779:Salmonella enterica / Q000145:classification* / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification*; D012703:Serotyping; D013552:Swine / Q000382:microbiology*
chemical_list: D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial
references.citation: Euro Surveill. 2008 Jun 12;13(24):
references.pmid: 18761944
references.citation: Geogr Med. 1988;18:171-4
references.pmid: 3262554
references.citation: Antimicrob Agents Chemother. 2011 Mar;55(3):1148-54
references.pmid: 21199924
references.citation: J Infect Dev Ctries. 2010 May 01;4(4):243-8
references.pmid: 20440063
references.citation: Foodborne Pathog Dis. 2005 Fall;2(3):263-73
references.pmid: 16156707
references.citation: Foodborne Pathog Dis. 2008 Oct;5(5):605-19
references.pmid: 18687034
references.citation: J Korean Med Sci. 2010 Dec;25(12):1693-9
references.pmid: 21165281
references.citation: Clin Infect Dis. 2009 Jul 1;49(1):132-41
references.pmid: 19489713
references.citation: J Appl Microbiol. 2004;96(4):750-60
references.pmid: 15012813
references.citation: J Infect Dis. 2011 Sep 1;204(5):675-84
references.pmid: 21813512
references.citation: Int J Food Microbiol. 2007 Sep 15;118(2):116-25
references.pmid: 17683820
references.citation: J Clin Microbiol. 2000 Dec;38(12):4633-6
references.pmid: 11101609
references.citation: Obstet Gynecol. 2006 Feb;107(2 Pt 2):516-8
references.pmid: 16449170
references.citation: J Antimicrob Chemother. 2011 Jun;66(6):1269-72
references.pmid: 21393162
references.citation: Zentralbl Bakteriol. 1996 Mar;283(3):391-8
references.pmid: 8861877
references.citation: Vet Res. 2011 Feb 14;42:34
references.pmid: 21324103
references.citation: Epidemiol Infect. 2012 Oct;140(10):1757-72
references.pmid: 22166269
references.citation: Curr Opin Infect Dis. 2008 Oct;21(5):531-8
references.pmid: 18725804
references.citation: Vet Rec. 2009 Nov 28;165(22):648-57
references.pmid: 19946125
references.citation: Z Gastroenterol. 2005 Aug;43(8):707-13
references.pmid: 16088767
references.citation: Epidemiol Infect. 2008 Aug;136(8):1118-23
references.pmid: 17961277
references.citation: Int J Food Microbiol. 2012 Feb 1;153(1-2):45-52
references.pmid: 22119458
references.citation: Emerg Infect Dis. 2005 Jun;11(6):943-6
references.pmid: 15963293
references.citation: Antimicrob Agents Chemother. 2009 Feb;53(2):603-8
references.pmid: 19029321
references.citation: J Antimicrob Chemother. 2009 Feb;63(2):274-81
references.pmid: 19001452
references.citation: Emerg Infect Dis. 1999 Sep-Oct;5(5):607-25
references.pmid: 10511517
references.citation: Antimicrob Agents Chemother. 2005 Jul;49(7):3001-3
references.pmid: 15980384
references.citation: J Infect Dev Ctries. 2010 Sep 03;4(8):484-94
references.pmid: 20818100
references.citation: Foodborne Pathog Dis. 2004 Winter;1(4):202-15
references.pmid: 15992282
references.citation: Environ Microbiol. 2004 Aug;6(8):868-71
references.pmid: 15250889
references.citation: Foodborne Pathog Dis. 2009 Oct;6(8):1009-19
references.pmid: 19735204
references.citation: Emerg Infect Dis. 2009 Jul;15(7):e2
references.pmid: 19624911