title: New coronary retroinfusion technique in the Rat infarct model: transjugular cardiac vein catheterization.
abstract: Cell delivery via the retrograde coronary route boasts less vessel embolism, myocardial injury, and arrhythmogenicity when compared with those via antegrade coronary administration or myocardial injection. However, conventional insertion into the coronary sinus and consequent bleeding complication prevent its application in small animals. To overcome the complication of bleeding, we described a modified coronary retroinfusion technique via the jugular vein route in rats with myocardial infarction (MI). A flexible wire with a bent end was inserted into the left internal jugular vein and advanced slowly along the left superior vena cava. Under direct vision, the wire was run into the left cardiac vein by rotating the wire and changing the position of its tip. A fine tube was then advanced along the wire to the left cardiac vein. This modified technique showed less lethal hemorrhage than the conventional technique. Retroinfusion via transjugular catheter enabled efficient fluid or cell dissemination to the majority areas of the free wall of the left ventricle, covering the infarcted anterior wall. In conclusion, transjugular cardiac vein catheterization may make retrocoronary infusion a more safe and practical route for delivering cell, drug, and gene therapy into the infarcted myocardium of rats.
authors.affiliation: Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital, Fudan University, 180 Feng Lin Road, Shanghai 200032, PR China.
authors.forename: Zheyong
authors.initials: Z
authors.lastname: Huang
authors.forename: Yunli
authors.initials: Y
authors.lastname: Shen
authors.forename: Hongmin
authors.initials: H
authors.lastname: Zhu
authors.forename: Jianfeng
authors.initials: J
authors.lastname: Xu
authors.forename: Yanan
authors.initials: Y
authors.lastname: Song
authors.forename: Xinying
authors.initials: X
authors.lastname: Hu
authors.forename: Zhang
authors.initials: Z
authors.lastname: Shuning
authors.forename: Xiangdong
authors.initials: X
authors.lastname: Yang
authors.forename: Aijun
authors.initials: A
authors.lastname: Sun
authors.forename: Juying
authors.initials: J
authors.lastname: Qian
authors.forename: Junbo
authors.initials: J
authors.lastname: Ge
mesh_terms: D000818:Animals; D002405:Catheterization, Central Venous / Q000009:adverse effects / Q000379:methods*; D017690:Cell Transplantation / Q000009:adverse effects / Q000379:methods*; D003331:Coronary Vessels; D004195:Disease Models, Animal; D006352:Heart Ventricles; D006470:Hemorrhage / Q000209:etiology / Q000517:prevention & control; D007601:Jugular Veins; D009203:Myocardial Infarction / Q000628:therapy*; D051381:Rats; D017207:Rats, Sprague-Dawley
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