title: [Effect of flavonoids of polygoni orientails fructus on human hepatoma cell line SMMC-7721].
abstract: OBJECTIVE
  To investigate the inhibitory effects of flavonoids of Polygoni Orientails Fructus on cell cycle and apoptosis of human hepatoma cell line SMMC-7721.
  
  
  METHODS
  MTT method was used to study the inhibitory rate and time-dose relationship of flavonoids on SMMC-7721. Flow cytometry PI staining and Annexin V-EGFP/PI double staining were used to measure the DNA concentration and apoptosis rate in SMMC-7721.
  
  
  RESULTS
  Flavonoids of Polygoni Orientails Fructus had obvious inhibitory effect and with good linear relationship between time and dose. Flavonoids of Polygoni Orientails Fructus could regulate the G1/S transition in hepatoma cell, which could make the cell in S phase arrest, result in the accumulation of cells in S phase, blocked DNA synthesis and replication of cells, blocked tumor cells into the G2/M phase, so as to restrain the proliferation of tumor cells and induce the apoptosis of SMMC-7721 cells. And this inductive effect showed obvious time-dose-effect relationship.
  
  
  CONCLUSIONS
  The flavonoids of Polygoni Orientails Fructus could inhibit the hepatoma cell line SMMC-7721 in vitro by inhibiting cell proliferation and inducing apoptosis of the cell in a time-dose dependence maner.
authors.affiliation: College of Pharmacy, Liaoning University of Traditional Chinese Medicine, Dalian 116600, China. byr1026@163.com
authors.forename: Yong-Rui
authors.initials: YR
authors.lastname: Bao
authors.forename: Shuai
authors.initials: S
authors.lastname: Wang
authors.forename: Xian-Sheng
authors.initials: XS
authors.lastname: Meng
authors.forename: Jing
authors.initials: J
authors.lastname: Chou
authors.forename: Xin-Xin
authors.initials: XX
authors.lastname: Yang
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000008:administration & dosage / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D005419:Flavonoids / Q000008:administration & dosage / Q000494:pharmacology*; D005434:Flow Cytometry; D016195:G2 Phase / Q000187:drug effects; D006801:Humans; D008113:Liver Neoplasms / Q000378:metabolism / Q000473:pathology*; D019664:Polygonaceae / Q000737:chemistry*; D013194:Staining and Labeling; D013997:Time Factors
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D005419:Flavonoids