title: Effects of inoculum to substrate ratio, substrate mix ratio and inoculum source on batch co-digestion of grass and pig manure.
abstract: Biochemical methane potential (BMP) assay was conducted at 35 °C to evaluate the effects of inoculum to substrate ratio (ISR) and substrate mix ratio between para-grass and pig manure co-digesting using different inocula. Rubber latex digester (RLD) inoculum showed higher methanogenic activity (41.4 mL CH4/gVS) than pig farm digester (PFD) inoculum (37.3 mL CH4/gVS). However, the maximum methane yields, occurred at the highest para-grass mix ratio (G), were 369.6, 437.6, 465.9 and 442.6 mL CH4/gTSadded for RLD inoculum, versus 332.4, 475.0, 519.5 and 521.9 mL/gTSadded for PFD inoculum at ISR 1, 2, 3, and 4, respectively. HPr, HBu and HVa appeared at higher G, corresponding to substrate's higher biodegradability. Response surface indicated that higher ISR and G had a significantly positive impact on methane yield. It suggested the use of higher ISR, i.e. 3 or 4, for BMP assay of these co-substrates. Dominant species of fermentative bacteria in each inoculum was tested by DGGE.
authors.affiliation: International Postgraduate Program in Environmental Management, Graduate School, Chulalongkorn University, Bangkok 10330, Thailand; Center of Excellence on Hazardous Substance Management (HSM), Bangkok 10330, Thailand.
authors.forename: Sumeth
authors.initials: S
authors.lastname: Dechrugsa
authors.affiliation: Department of Microbiology, Faculty of Science, Prince of Songkla University, Hat Yai Campus, Hat Yai, Songkhla 90110, Thailand.
authors.forename: Duangporn
authors.initials: D
authors.lastname: Kantachote
authors.affiliation: Energy Technology Research Center, Department of Civil Engineering, Faculty of Engineering, Prince of Songkla University, Hat Yai Campus, Hat Yai, Songkhla 90110, Thailand; Center of Excellence for Environmental and Hazardous Waste Management (EHWM), Southern University Consortium, Prince of Songkla University, Hat Yai Campus, Hat Yai, Songkhla 90110, Thailand. Electronic address: sumate.ch@psu.ac.th.
authors.forename: Sumate
authors.initials: S
authors.lastname: Chaiprapat
mesh_terms: D000818:Animals; D001673:Biodegradation, Environmental; D056804:Biofuels; D019149:Bioreactors; D002482:Cellulose / Q000737:chemistry*; D058645:Denaturing Gradient Gel Electrophoresis; D005227:Fatty Acids / Q000737:chemistry; D005285:Fermentation; D006863:Hydrogen-Ion Concentration; D007840:Latex; D008031:Lignin / Q000737:chemistry; D008372:Manure; D008564:Membrane Potentials; D008697:Methane / Q000737:chemistry; D006109:Poaceae / Q000737:chemistry*; D012408:Rubber; D013552:Swine; D013696:Temperature; D013816:Thermodynamics; D013997:Time Factors; D018505:Waste Management / Q000379:methods
chemical_list: D056804:Biofuels; D005227:Fatty Acids; D007840:Latex; D008372:Manure; D002482:Cellulose; D008031:Lignin; D012408:Rubber; D008697:Methane
keywords: Biochemical methane potential (BMP); Co-digestion; Grass; Inoculum to substrate ratio (ISR); Pig manure