title: The vaccine candidate BLSOmp31 protects mice against Brucella canis infection.
abstract: Canine brucellosis represents a major reproductive problem worldwide and it is considered a zoonotic disease. New approaches are therefore urgently needed to develop an effective and safe immunization strategy against Brucella canis. In the present study, BALB/c mice were subcutaneously immunized with the recombinant chimera rBLSOmp31 formulated in different adjuvants. The different strategies induced a vigorous immunoglobulin G (IgG) response, with high titers of IgG1 as well as IgG2. Besides, spleen cells from rBLSOmp31-immunized mice produced gamma interferon and IL-4, suggesting the induction of a mixed Th1-Th2. Vaccination with rBLSOmp31-IFA formulation provided the best protection levels comparable with that given by control vaccines. None of the immunization strategies induced serological interference in diagnosis. Hitherto, this is the first report that a recombinant vaccine confers protection against B. canis in mice.
authors.affiliation: Laboratorio de Inmunología, Departamento SAMP, Centro de Investigación Veterinaria de Tandil (CIVETAN) (CONICET), Facultad de Ciencias Veterinarias, Universidad Nacional del Centro de la Provincia de Buenos Aires, Tandil, Argentina; Consejo Nacional de Investigaciones Científicas (CONICET), Argentina.
authors.forename: Maria
authors.initials: M
authors.lastname: Clausse
authors.forename: Alejandra G
authors.initials: AG
authors.lastname: Díaz
authors.forename: Giselle
authors.initials: G
authors.lastname: Ghersi
authors.forename: Vanesa
authors.initials: V
authors.lastname: Zylberman
authors.forename: Juliana
authors.initials: J
authors.lastname: Cassataro
authors.forename: Guillermo H
authors.initials: GH
authors.lastname: Giambartolomei
authors.forename: Fernando A
authors.initials: FA
authors.lastname: Goldbaum
authors.forename: Silvia M
authors.initials: SM
authors.lastname: Estein
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D002004:Brucella Vaccine / Q000008:administration & dosage* / Q000276:immunology*; D041002:Brucella canis / Q000276:immunology*; D002006:Brucellosis / Q000276:immunology / Q000517:prevention & control*; D004195:Disease Models, Animal; D005260:Female; D007074:Immunoglobulin G / Q000097:blood; D007279:Injections, Subcutaneous; D007371:Interferon-gamma / Q000378:metabolism; D015847:Interleukin-4 / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D013154:Spleen / Q000276:immunology
chemical_list: D000276:Adjuvants, Immunologic; D000907:Antibodies, Bacterial; D002004:Brucella Vaccine; D007074:Immunoglobulin G; D015847:Interleukin-4; D007371:Interferon-gamma
keywords: Adjuvants; Brucella canis; Chimera BLSOmp31; Mice; Protection; Vaccine