title: Differential protein expression in perfusates from metastasized rat livers.
abstract: BACKGROUND
  Liver perfusates exhibit theoretical advantages regarding the discovery of disease biomarkers because they contain proteins that readily enter the blood-stream, and perfusion preserves the disease state in its natural context. The purpose of the study is to explore the value of liver perfusate proteome in the biomarker discovery of liver diseases.
  
  
  RESULTS
  In this study, 86 differentially expressed proteins were identified in perfusates from isolated rat livers metastasized by Walker-256 tumor cells. Among these proteins, 27 were predicted to be secreted, and 59 were intracellular or membrane proteins. Most of the secretory proteins (70.4%) were decreased in metastasized liver perfusates. The main canonical ingenuity pathway to which these secretory proteins belonged was acute phase response, which indicated that the liver-associated immune reaction was damaged by the metastasis. In contrast, most of the intracellular or membrane proteins (86.4%) exhibited higher relative abundances in the metastasized liver perfusates. Some of these proteins, including Rpl21, Atic, Eif3s2, Echs1, Eps15 and Ywhab, have previously been reported to be involved in cancer genesis and progression. As a member of the 14-3-3 protein family, Ywhab plays a key role in cellular proliferation and oncogenic transformation and has been reported to be involved in the development of breast cancer. Its abundance was elevated by 3.5-fold in the metastasized perfusates. Validation by Western blotting revealed a 3.7-fold increase in the abundance of this protein in metastasized plasma.
  
  
  CONCLUSIONS
  These results show that perfusate proteome can be used as an alternative initial resource for biomarker identification, which ultimately requires validation in serum.
authors.affiliation: National Key Laboratory of Medical Molecular Biology, Department of Physiology and Pathophysiology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences/School of Basic Medicine, Peking Union Medical College, Beijing 100005, China.|Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: National Key Laboratory of Medical Molecular Biology, Department of Physiology and Pathophysiology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences/School of Basic Medicine, Peking Union Medical College, Beijing 100005, China.
authors.forename: Menglin
authors.initials: M
authors.lastname: Li
authors.affiliation: National Key Laboratory of Medical Molecular Biology, Department of Physiology and Pathophysiology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences/School of Basic Medicine, Peking Union Medical College, Beijing 100005, China.
authors.forename: Lilong
authors.initials: L
authors.lastname: Wei
authors.affiliation: National Key Laboratory of Medical Molecular Biology, Department of Physiology and Pathophysiology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences/School of Basic Medicine, Peking Union Medical College, Beijing 100005, China.
authors.forename: Lisi
authors.initials: L
authors.lastname: Zhu
authors.affiliation: National Key Laboratory of Medical Molecular Biology, Department of Physiology and Pathophysiology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences/School of Basic Medicine, Peking Union Medical College, Beijing 100005, China.
authors.forename: Siqi
authors.initials: S
authors.lastname: Hu
authors.affiliation: Core Instrument Facility, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences/School of Basic Medicine, Peking Union Medical College, Beijing 100005, China.
authors.forename: Shuzhen
authors.initials: S
authors.lastname: Wu
authors.affiliation: National Key Laboratory of Medical Molecular Biology, Department of Physiology and Pathophysiology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences/School of Basic Medicine, Peking Union Medical College, Beijing 100005, China.
authors.forename: Sucan
authors.initials: S
authors.lastname: Ma
authors.affiliation: National Key Laboratory of Medical Molecular Biology, Department of Physiology and Pathophysiology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences/School of Basic Medicine, Peking Union Medical College, Beijing 100005, China.
authors.forename: Youhe
authors.initials: Y
authors.lastname: Gao
references.citation: J Chromatogr B Analyt Technol Biomed Life Sci. 2007 Apr 15;849(1-2):273-81
references.pmid: 17188586
references.citation: Hepatology. 1986 May-Jun;6(3):511-7
references.pmid: 3519420
references.citation: Anal Biochem. 1976 May 7;72:248-54
references.pmid: 942051
references.citation: J Mol Biol. 2004 Jul 16;340(4):783-95
references.pmid: 15223320
references.citation: J Biol Chem. 2003 Feb 21;278(8):6160-7
references.pmid: 12456677
references.citation: Cancer Res. 1989 Jul 15;49(14):3904-9
references.pmid: 2736531
references.citation: J Immunol. 1987 Nov 1;139(9):3132-8
references.pmid: 3312414
references.citation: Anal Chem. 2003 Sep 1;75(17):4646-58
references.pmid: 14632076
references.citation: J Transl Med. 2008 Sep 17;6:52
references.pmid: 18796163
references.citation: Carcinogenesis. 2000 Nov;21(11):2073-7
references.pmid: 11062170
references.citation: J Proteome Res. 2010 Apr 5;9(4):1894-901
references.pmid: 20148517
references.citation: Anal Chem. 2002 Oct 15;74(20):5383-92
references.pmid: 12403597
references.citation: Nat Protoc. 2007;2(4):953-71
references.pmid: 17446895
references.citation: Oncogene. 2004 Feb 19;23(7):1377-91
references.pmid: 14973550
references.citation: N Engl J Med. 1990 Feb 1;322(5):297-302
references.pmid: 2296271
references.citation: Eur J Appl Physiol. 2008 Dec;104(6):957-64
references.pmid: 18688637
references.citation: Biochim Biophys Acta. 2004 Apr 5;1688(3):187-96
references.pmid: 15062868
references.citation: J Exp Med. 1954 Feb;99(2):133-53
references.pmid: 13130790
references.citation: Lab Anim. 2006 Jul;40(3):236-46
references.pmid: 16803641
references.citation: Biochem Biophys Res Commun. 2004 Mar 19;315(4):950-8
references.pmid: 14985104
references.citation: Proteomics. 2005 Dec;5(18):4994-5001
references.pmid: 16281181
references.citation: Lancet. 1989 Nov 11;2(8672):1115-8
references.pmid: 2572846
references.citation: Neurosci Lett. 2010 Mar 31;473(1):1-4
references.pmid: 19539710
references.citation: J Exp Med. 1954 Feb;99(2):125-32
references.pmid: 13130789
references.citation: Nat Biotechnol. 2006 Aug;24(8):971-83
references.pmid: 16900146
references.citation: Genome Biol. 2008;9(6):R93
references.pmid: 18522751
references.citation: J Cell Biochem. 1987 Sep;35(1):17-29
references.pmid: 3312245
references.citation: Int J Cancer. 2007 Dec 15;121(12):2596-605
references.pmid: 17722004
references.citation: J Proteome Res. 2006 Jan;5(1):177-82
references.pmid: 16396509
references.citation: Biochim Biophys Acta. 2000 Jun 21;1492(1):203-6
references.pmid: 10858549
references.citation: Genomics. 2003 Jul;82(1):78-85
references.pmid: 12809678