title: Actin expression in trypanosomatids (Euglenozoa: Kinetoplastea).
abstract: Heteroxenic and monoxenic trypanosomatids were screened for the presence of actin using a mouse polyclonal antibody produced against the entire sequence of the Trypanosoma cruzi actin gene, encoding a 41.9 kDa protein. Western blot analysis showed that this antibody reacted with a polypeptide of approximately 42 kDa in the whole-cell lysates of parasites targeting mammals (T. cruzi, Trypanosoma brucei and Leishmania major), insects (Angomonas deanei, Crithidia fasciculata, Herpetomonas samuelpessoai and Strigomonas culicis) and plants (Phytomonas serpens). A single polypeptide of approximately 42 kDa was detected in the whole-cell lysates of T. cruzi cultured epimastigotes, metacyclic trypomastigotes and amastigotes at similar protein expression levels. Confocal microscopy showed that actin was expressed throughout the cytoplasm of all the tested trypanosomatids. These data demonstrate that actin expression is widespread in trypanosomatids.
authors.affiliation: Instituto Carlos Chagas-Fiocruz, Curitiba, PR, Brasil.
authors.forename: Ligia Cristina Kalb
authors.initials: LC
authors.lastname: Souza
authors.forename: Rosana Elisa Gonçalves Gonçalves
authors.initials: RE
authors.lastname: Pinho
authors.forename: Carla Vanessa de Paula
authors.initials: CV
authors.lastname: Lima
authors.forename: Stênio Perdigão
authors.initials: SP
authors.lastname: Fragoso
authors.forename: Maurilio José
authors.initials: MJ
authors.lastname: Soares
mesh_terms: D000199:Actins / Q000378:metabolism*; D000818:Animals; D015153:Blotting, Western; D004591:Electrophoresis, Polyacrylamide Gel; D005455:Fluorescent Antibody Technique; D051379:Mice; D018613:Microscopy, Confocal; D015800:Protozoan Proteins / Q000378:metabolism; D014351:Trypanosomatina / Q000378:metabolism*
chemical_list: D000199:Actins; D015800:Protozoan Proteins
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