title: The components of Drosophila histone chaperone dCAF-1 are required for the cell death phenotype associated with rbf1 mutation.
abstract: A Polycomb group protein, Posterior sex combs (Psc), was identified in a genetic screen designed to find factors that can specifically induce morphological defects in rbf1 mutant eyes. We discovered that rbf1 mutations enhance developmental phenotypes caused by Psc overexpression such as ectopic cell death and disorganized ommatidia. Our genetic analysis revealed that Psc-induced developmental defects are strongly influenced by CAF1p55, which is a shared component of several chromatin-associated complexes including a histone chaperone complex, chromatin assembly factor-1 (dCAF-1). Interestingly, the expression levels of dCAF-1 components, CAF1p105 and CAF1p180, are increased in rbf1 mutants, whereas the expression level of CAF1p55 itself remains relatively unchanged. We demonstrated that the increased levels of CAF1p105 and CAF1p180 are required for the hypersensitivity of rbf1 mutant cells to Psc-induced cell death and for the developmentally regulated cell death normally observed in rbf1 mutant eyes. We propose that Caf1p105 and Caf1p180 are important determinants of cell death sensitivity in rbf1 mutant cells and contribute to the genetic interaction between Psc and rbf1.
authors.affiliation: Department of Biology, Developmental Biology Research Initiative, McGill University, Montreal, Quebec H3A 1B1, Canada.
authors.forename: Heather
authors.initials: H
authors.lastname: Collins
authors.forename: Nam-Sung
authors.initials: NS
authors.lastname: Moon
mesh_terms: D000818:Animals; D016923:Cell Death; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D004330:Drosophila / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D009154:Mutation; D010641:Phenotype; D063150:Polycomb Repressive Complex 1 / Q000235:genetics / Q000378:metabolism; D016160:Retinoblastoma Protein; D056505:Retinoblastoma-Binding Protein 4 / Q000235:genetics / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: C110251:Caf1-55 protein, Drosophila; D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; C099965:Rbf protein, Drosophila; D016160:Retinoblastoma Protein; D056505:Retinoblastoma-Binding Protein 4; D014157:Transcription Factors; C071050:Psc protein, Drosophila; D063150:Polycomb Repressive Complex 1
keywords: cell death; chromatin assembly factor-1; posterior sex combs; rbf1
references.citation: Methods Mol Biol. 2000;132:365-86
references.pmid: 10547847
references.citation: Nature. 1986 Oct 16-22;323(6089):643-6
references.pmid: 2877398
references.citation: Development. 2011 May;138(10):1957-66
references.pmid: 21490066
references.citation: Mol Cell Biol. 2005 Aug;25(15):6578-91
references.pmid: 16024794
references.citation: Genes Dev. 2004 Jul 15;18(14):1667-80
references.pmid: 15256498
references.citation: Cell. 2004 Oct 15;119(2):181-93
references.pmid: 15479636
references.citation: Dev Biol. 2007 Nov 1;311(1):213-22
references.pmid: 17916346
references.citation: Cancer Sci. 2010 Jul;101(7):1754-60
references.pmid: 20426791
references.citation: Genes Dev. 2001 Aug 15;15(16):2146-60
references.pmid: 11511545
references.citation: Genes Dev. 2008 May 15;22(10):1313-8
references.pmid: 18443147
references.citation: Nature. 1992 Sep 24;359(6393):288-94
references.pmid: 1406932
references.citation: Genes Dev. 2011 Feb 15;25(4):323-35
references.pmid: 21325133
references.citation: Nature. 1992 Sep 24;359(6393):295-300
references.pmid: 1406933
references.citation: Genetics. 2004 Jan;166(1):187-200
references.pmid: 15020417
references.citation: Nat Rev Mol Cell Biol. 2009 Oct;10(10):697-708
references.pmid: 19738629
references.citation: Nature. 2012 Jan 11;481(7381):329-34
references.pmid: 22237022
references.citation: BMC Cancer. 2009 Feb 19;9:61
references.pmid: 19228380
references.citation: EMBO J. 2002 Sep 16;21(18):4927-37
references.pmid: 12234932
references.citation: Nat Rev Mol Cell Biol. 2008 Sep;9(9):713-24
references.pmid: 18719710
references.citation: Mol Cell Biol. 2001 Oct;21(19):6574-84
references.pmid: 11533245
references.citation: Nat Rev Cancer. 2006 Nov;6(11):846-56
references.pmid: 17060944
references.citation: Dev Biol. 1994 Feb;161(2):379-92
references.pmid: 8313990
references.citation: Nature. 2006 Nov 2;444(7115):61-6
references.pmid: 17080083
references.citation: J Biol Chem. 2011 Jul 1;286(26):23388-96
references.pmid: 21550984
references.citation: Cancer Lett. 1996 Aug 23;106(1):75-82
references.pmid: 8827049
references.citation: Nat Genet. 2004 Mar;36(3):283-7
references.pmid: 14981521
references.citation: EMBO J. 1996 Nov 15;15(22):6178-88
references.pmid: 8947040
references.citation: Nature. 1992 Sep 24;359(6393):328-30
references.pmid: 1406937
references.citation: Cell Mol Life Sci. 2008 Feb;65(3):414-44
references.pmid: 17955179
references.citation: EMBO J. 1999 Feb 15;18(4):916-25
references.pmid: 10022834
references.citation: J Cell Sci. 2010 Aug 15;123(Pt 16):2853-61
references.pmid: 20663913
references.citation: Methods. 2001 Dec;25(4):402-8
references.pmid: 11846609
references.citation: Mol Cell Biol. 1996 Mar;16(3):810-7
references.pmid: 8622682
references.citation: Nature. 2010 Aug 26;466(7310):1110-4
references.pmid: 20686481
references.citation: Proc Natl Acad Sci U S A. 1998 Jan 6;95(1):132-7
references.pmid: 9419341
references.citation: Mol Cell Biol. 2006 Oct;26(20):7601-15
references.pmid: 16954388
references.citation: J Cell Sci. 2011 Dec 15;124(Pt 24):4203-12
references.pmid: 22193959
references.citation: Nature. 2000 Nov 16;408(6810):307-10
references.pmid: 11099028
references.citation: Mol Cell Biol. 2005 May;25(9):3563-74
references.pmid: 15831462
references.citation: PLoS Genet. 2010 Aug 19;6(8):e1001071
references.pmid: 20808898
references.citation: Mol Cancer. 2011 Jan 28;10(1):10
references.pmid: 21276221