title: Investigation of mtDNA control region sequences in an Egyptian population sample.
abstract: The sequences of mitochondrial DNA (mtDNA) control region were investigated in 101 unrelated individuals living in the northern region of Nile delta (Gharbia, N=55 and Kafrelsheikh, N=46). DNA was extracted from blood stained filter papers or buccal swabs. HV1, HV2 and HV3 were PCR amplified and sequenced; the resulted sequences were aligned and compared with revised Cambridge sequence (rCRS). The results revealed presence of total 93 different haplotypes, 86 of them are unique and 7 are shared haplotypes, the most common haplotype, was observed with a frequency, 2.97% of population sample. High mtDNA diversity was observed with genetic diversity and power of discrimination, 0.9982 and 0.9883, respectively. In this dataset the west Eurasian haplogroups predominated over the African haplogroups. The results would be useful for forensic examinations and human genetic studies.
authors.affiliation: Department of Legal Medicine, Graduate School of Medicine, Gifu University, Gifu, Japan; Department of Forensic Medicine and Toxicology, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafrelsheikh, Egypt. Electronic address: drmostox@yahoo.com.
authors.forename: Mostafa Ali
authors.initials: MA
authors.lastname: Elmadawy
authors.forename: Atsushi
authors.initials: A
authors.lastname: Nagai
authors.forename: Ghada M
authors.initials: GM
authors.lastname: Gomaa
authors.forename: Hanaa M R
authors.initials: HM
authors.lastname: Hegazy
authors.forename: Fawzy Eid
authors.initials: FE
authors.lastname: Shaaban
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Bunai
mesh_terms: D022801:Complementarity Determining Regions / Q000235:genetics*; D004272:DNA, Mitochondrial / Q000235:genetics*; D004534:Egypt; D014644:Genetic Variation; D005828:Genetics, Population / Q000379:methods*; D006239:Haplotypes; D063426:Human Migration; D006801:Humans; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA / Q000379:methods
chemical_list: D022801:Complementarity Determining Regions; D004272:DNA, Mitochondrial
keywords: Egypt delta; Forensic data; Hyper variable regions; Mitochondrial DNA