title: Microbial community structure across a wastewater-impacted riparian buffer zone in the southeastern coastal plain.
abstract: Riparian buffer zones are important for both natural and developed ecosystems throughout the world because of their ability to retain nutrients, prevent soil erosion, protect aquatic environments from excessive sedimentation, and filter pollutants. Despite their importance, the microbial community structures of riparian buffer zones remains poorly defined. Our objectives for this study were twofold: first, to characterize the microbial populations found in riparian buffer zone soils; and second, to determine if microbial community structure could be linked to denitrification enzyme activity (DEA). To achieve these objectives, we investigated the microbial populations of a riparian buffer zone located downslope of a pasture irrigated with swine lagoon effluent, utilizing DNA sequencing of the 16S rDNA, DEA, and quantitative PCR (qPCR) of the denitrification genes nirK, nirS, and nosZ. Clone libraries of the 16S rDNA gene were generated from each of twelve sites across the riparian buffer with a total of 986 partial sequences grouped into 654 operational taxonomic units (OTUs). The Proteobacteria were the dominant group (49.8% of all OTUs), with the Acidobacteria also well represented (19.57% of all OTUs). Analysis of qPCR results identified spatial relationships between soil series, site location, and gene abundance, which could be used to infer both incomplete and total DEA rates.
authors.affiliation: Coastal Plains Soil, Water, and Plant Research Center, Agricultural Research Service, USDA, Florence SC.
authors.forename: T F
authors.initials: TF
authors.lastname: Ducey
authors.forename: P R
authors.initials: PR
authors.lastname: Johnson
authors.forename: A D
authors.initials: AD
authors.lastname: Shriner
authors.forename: T A
authors.initials: TA
authors.lastname: Matheny
authors.forename: P G
authors.initials: PG
authors.lastname: Hunt
keywords: 16S rRNA gene.; Riparian buffer; denitrification; microbial communities
references.citation: FEMS Microbiol Ecol. 2004 Sep 1;49(3):401-17
references.pmid: 19712290
references.citation: FEMS Microbiol Lett. 1998 May 1;162(1):61-8
references.pmid: 9595664
references.citation: Appl Environ Microbiol. 1998 Oct;64(10):3769-75
references.pmid: 9758798
references.citation: Microbiology (Reading). 1996 Oct;142 ( Pt 10):2863-70
references.pmid: 8885402
references.citation: Mol Biol Evol. 2007 Aug;24(8):1596-9
references.pmid: 17488738
references.citation: J Environ Qual. 2004 Nov-Dec;33(6):2367-74
references.pmid: 15537960
references.citation: Syst Appl Microbiol. 2003 Nov;26(4):529-38
references.pmid: 14666981
references.citation: Appl Environ Microbiol. 1999 Apr;65(4):1721-30
references.pmid: 10103273
references.citation: Appl Environ Microbiol. 2000 Jan;66(1):230-7
references.pmid: 10618229
references.citation: Environ Pollut. 2010 Oct;158(10):3225-9
references.pmid: 20724046
references.citation: Appl Environ Microbiol. 1997 Jul;63(7):2593-9
references.pmid: 9212409
references.citation: FEMS Microbiol Lett. 2008 Aug;285(2):263-9
references.pmid: 18557943
references.citation: Proc Natl Acad Sci U S A. 2008 Nov 18;105(46):17842-7
references.pmid: 19004771
references.citation: Bioinformatics. 2004 Sep 22;20(14):2317-9
references.pmid: 15073015
references.citation: J Environ Qual. 2011 Mar-Apr;40(2):610-9
references.pmid: 21520768
references.citation: Appl Environ Microbiol. 2008 Dec;74(24):7585-95
references.pmid: 18978080
references.citation: J Bacteriol. 1989 Dec;171(12):6455-67
references.pmid: 2592342
references.citation: Appl Environ Microbiol. 2007 Aug;73(16):5261-7
references.pmid: 17586664
references.citation: Environ Monit Assess. 1996 Sep;42(1-2):99-112
references.pmid: 24193495
references.citation: ISME J. 2009 Mar;3(3):305-13
references.pmid: 18987677
references.citation: Science. 2002 May 10;296(5570):1064-6
references.pmid: 12004116
references.citation: Appl Environ Microbiol. 2006 Sep;72(9):5957-62
references.pmid: 16957216
references.citation: Science. 1987 Jul 3;237(4810):35-42
references.pmid: 17813621
references.citation: ISME J. 2009 Apr;3(4):442-53
references.pmid: 19129864
references.citation: Curr Opin Biotechnol. 2006 Jun;17(3):241-9
references.pmid: 16704931
references.citation: Nucleic Acids Res. 2001 May 1;29(9):e45
references.pmid: 11328886
references.citation: Appl Environ Microbiol. 1997 Dec;63(12):4704-12
references.pmid: 9406389
references.citation: Appl Environ Microbiol. 2003 Aug;69(8):4549-55
references.pmid: 12902241
references.citation: Nucleic Acids Res. 2001 Jan 1;29(1):181-4
references.pmid: 11125085
references.citation: J Microbiol Methods. 2006 Jul;66(1):147-55
references.pmid: 16343671
references.citation: Nat Rev Microbiol. 2005 Sep;3(9):733-9
references.pmid: 16138101
references.citation: J Environ Qual. 2007 Jul 17;36(5):1368-76
references.pmid: 17636299
references.citation: Mol Biol Evol. 2008 Sep;25(9):1955-66
references.pmid: 18614527
references.citation: J Mol Biol. 1990 Oct 5;215(3):403-10
references.pmid: 2231712
references.citation: Nucleic Acids Res. 2007 Jan;35(Database issue):D169-72
references.pmid: 17090583
references.citation: Nucleic Acids Res. 2004 Mar 19;32(5):1792-7
references.pmid: 15034147