title: Phosphorylation and anti-tumor activity of exopolysaccharide from Lachnum YM120.
abstract: Phosphorylated polysaccharide PLEP-1a, with the PO₄³⁻ content of 6.39%, was prepared from LEP-1a by phosphorylation. IR, (13)C NMR and (31)P NMR results of PLEP-1a showed that the original basic structure of the polysaccharide was not changed, and the -H₂PO₃ group was linked at C₆ of LEP-1a. The results of anti-tumor experiments in vivo showed that 100 mg/kg and 400 mg/kg of LEP-1a could significantly improve the food consumption, body weight, tumor inhibition rate and thymus index of S180 sarcoma mice, and increase the levels of SOD, IL-2 and TNF-α in mice blood serum, indicating that LEP-1a had an excellent anti-tumor activity. Furthermore, PLEP-1a had a significantly enhanced inhibitory effect on S180 sarcoma mice than LEP-1a, suggesting that phosphorylation is an effective way of improving the biological activity of LEP-1a.
authors.affiliation: College of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009, China. yeming123@sina.com
authors.forename: Ming
authors.initials: M
authors.lastname: Ye
authors.forename: Ru-yue
authors.initials: RY
authors.lastname: Yuan
authors.forename: Yun-long
authors.initials: YL
authors.lastname: He
authors.forename: Zhan-zhan
authors.initials: ZZ
authors.lastname: Du
authors.forename: Xiao-jing
authors.initials: XJ
authors.lastname: Ma
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D001203:Ascomycota / Q000737:chemistry*; D005247:Feeding Behavior / Q000187:drug effects; D005260:Female; D007376:Interleukin-2 / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D051379:Mice; D009928:Organ Specificity / Q000187:drug effects; D010710:Phosphates / Q000032:analysis; D010766:Phosphorylation / Q000187:drug effects; D011134:Polysaccharides / Q000494:pharmacology*; D012015:Reference Standards; D012510:Sarcoma 180 / Q000201:enzymology / Q000473:pathology; D013055:Spectrophotometry, Infrared; D013482:Superoxide Dismutase / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D015430:Weight Gain / Q000187:drug effects
chemical_list: D000970:Antineoplastic Agents; D007376:Interleukin-2; D010710:Phosphates; D011134:Polysaccharides; D014409:Tumor Necrosis Factor-alpha; D013482:Superoxide Dismutase
keywords: Anti-tumor activity; Exopolysaccharide; Lachnum YM120; Phosphorylation