title: [A comparative study of the gene chip technology and acid-fast staining or mycobacterial culture to diagnose].
abstract: OBJECTIVE
  To investigate the clinical value of mycobacterial DNA microarray technology for diagnosis of childhood tuberculosis.
  
  
  METHODS
  120 clinical specimens were collected from hospitalized child patients. Acid-fast staining, mycobacterial culture and DNA microarray assays were performed using these clinical specimens. The results of DNA microarray assays were compared with the results of acid-fast staining and mycobacterial culture.
  
  
  RESULTS
  The sensitivity of DNA microarray assays for specimens from children with tuberculosis was 24.3% (17/70), of acid-fast staining 17.1% (12/70), of mycobacterial culture 20.0% (14/70), and the specificity of the three methods was all 100.0% (50/50). The difference between results of DNA microarray assays and that of acid-fast staining or mycobacterial culture was not significant.
  
  
  CONCLUSIONS
  DNA microarray assay has reference value for the diagnosis of childhood tuberculosis. It provides a new way for the diagnosis of childhood tuberculosis.
authors.affiliation: Department of Pediatrics, West China Second Hospital, Sichuan University, Chengdu 610041, China.
authors.forename: Jian-Jun
authors.initials: JJ
authors.lastname: Deng
authors.forename: Guo-Guang
authors.initials: GG
authors.lastname: Xiao
authors.forename: Chao-Min
authors.initials: CM
authors.lastname: Wan
authors.forename: Chong-Liang
authors.initials: CL
authors.lastname: Zeng
authors.forename: Ling
authors.initials: L
authors.lastname: Cao
authors.forename: Min
authors.initials: M
authors.lastname: Shu
authors.forename: De-Zhi
authors.initials: DZ
authors.lastname: Mu
authors.forename: Wei
authors.initials: W
authors.lastname: Zhou
mesh_terms: D002648:Child; D046508:Culture Techniques / Q000379:methods; D004269:DNA, Bacterial / Q000032:analysis; D005260:Female; D006801:Humans; D008297:Male; D009169:Mycobacterium tuberculosis / Q000235:genetics / Q000302:isolation & purification*; D020411:Oligonucleotide Array Sequence Analysis; D013194:Staining and Labeling; D014376:Tuberculosis / Q000175:diagnosis* / Q000382:microbiology*
chemical_list: D004269:DNA, Bacterial