title: Development of a liquid chromatography-tandem mass spectrometry procedure for determination of endocrine disrupting compounds in fish from Mediterranean rivers.
abstract: A new, sensitive and rapid method based on QuEChERS (Quick, Easy, Cheap, Effective, Rugged and Safe) approach followed by ultra high performance liquid chromatography coupled with tandem mass spectrometry (UHPLC-MS/MS) was developed for the determination of nineteen endocrine disruptors (EDCs) and related compounds belonging to different classes in various fish species. Matrix effect on the analytical performance was evaluated, and thus, internal sample calibration was chosen as the most appropriate approach when analyzing such complex matrices as biota. The procedure provided adequate recoveries in the range from 40% to 103% for most of the compounds, low method detection limits (MDLs) in the range from 0.002 to 3.09ng/g for fish homogenates and high accuracy <20%. The developed method was applied for the analysis of target compounds in homogenates of different fish species from four impacted Mediterranean rivers: Ebro, Llobregat, Júcar and Guadalquivir. Eleven out of the nineteen target EDCs were found at least once in fish homogenates. Llobregat was identified as the most polluted river, where high concentrations were measured in fish homogenates especially for bisphenol A (223.91±11.51ng/g). Tris (2-butoxyethyl) phosphate (TBEP), caffeine, and methyl and benzyl paraben were found in fish from the four river basins.
authors.affiliation: Department of Analytical Chemistry, Chemical Faculty, Gdańsk University of Technology, 11/12 Narutowicza Street, 80-233 Gdańsk, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Jakimska
authors.forename: Belinda
authors.initials: B
authors.lastname: Huerta
authors.forename: Żaneta
authors.initials: Ż
authors.lastname: Bargańska
authors.forename: Agata
authors.initials: A
authors.lastname: Kot-Wasik
authors.forename: Sara
authors.initials: S
authors.lastname: Rodríguez-Mozaz
authors.forename: Damià
authors.initials: D
authors.lastname: Barceló
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D052244:Endocrine Disruptors / Q000032:analysis / Q000378:metabolism; D005399:Fishes / Q000378:metabolism; D019083:Mediterranean Region; D018482:Muscle, Skeletal / Q000737:chemistry* / Q000378:metabolism; D045483:Rivers / Q000737:chemistry; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014874:Water Pollutants, Chemical / Q000032:analysis* / Q000378:metabolism
chemical_list: D052244:Endocrine Disruptors; D014874:Water Pollutants, Chemical
keywords: Endocrine disrupting compounds; Fish; Hormones; QuEChERS; UHPLC–MS/MS