title: Deficiency of cartilage oligomeric matrix protein causes dilated cardiomyopathy.
abstract: Alterations in cardiac extracellular matrix are involved in dilated cardiomyopathy (DCM) and its progression to heart failure. The matricellular protein cartilage oligomeric matrix protein (COMP) has been indicated localized in the heart. However, the role of COMP in cardiac homeostasis and disease remains elusive. COMP (-/-) mice, both male and female, developed DCM spontaneously at young age (3-5 months), with impaired cardiac function. Assessment of postnatal COMP (-/-) heart at 1 month, although functionally normal, revealed severe cardiac ultrastructure defect, in parallel with cardiomyocyte apoptosis, myofilament loss, connexin-43 deficiency and matrix metalloproteinase activation. Decreased COMP expression was observed in the heart sample of DCM patients compared with donor heart. Mechanistically, COMP (-/-) heart exhibited reduced integrin β1 expression and signaling. Ectopic expression of COMP or integrin β1 rescued COMP-deficiency-induced cardiomyocyte apoptosis, myofilament dissolution, and connexin-43 aberrance. Additionally, COMP directly bonded to the extracellular β-tail domain of integrin β1, prevented integrin β1 ubiquitination/degradation, and maintained the cardiac homeostasis. COMP-integrin β1 axis is a potential target of DCM.
authors.affiliation: Department of Physiology and Pathophysiology, Basic Medical College of Peking University, Peking University, Beijing, 100191, People's Republic of China.
authors.forename: Yaqian
authors.initials: Y
authors.lastname: Huang
authors.forename: Jiahong
authors.initials: J
authors.lastname: Xia
authors.forename: Jingang
authors.initials: J
authors.lastname: Zheng
authors.forename: Bin
authors.initials: B
authors.lastname: Geng
authors.forename: Peng
authors.initials: P
authors.lastname: Liu
authors.forename: Fang
authors.initials: F
authors.lastname: Yu
authors.forename: Bo
authors.initials: B
authors.lastname: Liu
authors.forename: Hongquan
authors.initials: H
authors.lastname: Zhang
authors.forename: Mingjiang
authors.initials: M
authors.lastname: Xu
authors.forename: Ping
authors.initials: P
authors.lastname: Ye
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhu
authors.forename: Qingbo
authors.initials: Q
authors.lastname: Xu
authors.forename: Xian
authors.initials: X
authors.lastname: Wang
authors.forename: Wei
authors.initials: W
authors.lastname: Kong
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002311:Cardiomyopathy, Dilated / Q000209:etiology* / Q000378:metabolism* / Q000473:pathology*; D064236:Cartilage Oligomeric Matrix Protein; D005109:Extracellular Matrix / Q000378:metabolism; D016326:Extracellular Matrix Proteins / Q000172:deficiency*; D005260:Female; D006023:Glycoproteins / Q000172:deficiency*; D057809:HEK293 Cells; D006801:Humans; D007150:Immunohistochemistry; D047468:Immunoprecipitation; D020287:In Situ Nick-End Labeling; D008297:Male; D064235:Matrilin Proteins; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D032383:Myocytes, Cardiac / Q000378:metabolism / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D060888:Real-Time Polymerase Chain Reaction; D014162:Transfection
chemical_list: D064236:Cartilage Oligomeric Matrix Protein; D016326:Extracellular Matrix Proteins; D006023:Glycoproteins; C577540:Matn1 protein, mouse; D064235:Matrilin Proteins; C577553:TSP5 protein, human