title: Development of a novel lipidic nanoparticle probe using liposomal encapsulated Gd₂O₃-DEG for molecular MRI.
abstract: Recently, it has been showed that gadolinium oxide nanoparticles can provide high-contrast enhancement in magnetic resonance imaging (MRI). Moreover, liposomes due to high biocompatibility have shown unique model systems, with the most successful application being the drug delivery system. As a suitable cell-tracking contrast agent (CA) in molecular MRI (mMRI), the synthesis and optimisation characteristic of a novel paramagnetic liposomes (PMLs) based on gadolinium nanoparticles, essentially composed of a new complex of gadolinium oxide-diethylene glycol (Gd₂O₃-DEG) loaded in liposomes have been determined in this research. Gd₂O₃-DEG was prepared by a new supervised polyol method and was encapsulated with liposome by the film hydration method. The paramagnetic liposome nanoparticle (PMLN) sizes ranged from 65 to 170 nm. The r₁ of PMLNs and Gd₂O₃-DEG were much higher than that of Gd-diethylenetriamine penta-acetic acid (Gd-DTPA). In MC/9 cell lines, the experiments showed similar results as in water. PMLNs with lower T₁ than Gd-DTPA are sensitive, positive MRI CA that could be attractive candidates for cellular and molecular lipid content targets such as diagnostic applications.
authors.affiliation: Medical Physics Department, School of Medicine, Tehran University of Medical Sciences , Tehran , Iran.
authors.forename: R
authors.initials: R
authors.lastname: Zohdiaghdam
authors.forename: N
authors.initials: N
authors.lastname: Riyahi-Alam
authors.forename: H R
authors.initials: HR
authors.lastname: Moghimi
authors.forename: S
authors.initials: S
authors.lastname: Haghgoo
authors.forename: A
authors.initials: A
authors.lastname: Alinaghi
authors.forename: Gh
authors.initials: G
authors.lastname: Azizian
authors.forename: H
authors.initials: H
authors.lastname: Ghanaati
authors.forename: E
authors.initials: E
authors.lastname: Gorji
authors.forename: B
authors.initials: B
authors.lastname: Rafiei
mesh_terms: D000818:Animals; D002460:Cell Line; D003287:Contrast Media / Q000008:administration & dosage* / Q000032:analysis; D005026:Ethylene Glycols / Q000008:administration & dosage* / Q000032:analysis; D005682:Gadolinium / Q000008:administration & dosage* / Q000032:analysis; D008081:Liposomes / Q000737:chemistry* / Q000648:ultrastructure; D008279:Magnetic Resonance Imaging / Q000379:methods; D051379:Mice; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure
chemical_list: D003287:Contrast Media; D005026:Ethylene Glycols; D008081:Liposomes; C030581:gadolinium oxide; C013484:diethylene glycol; D005682:Gadolinium