title: Simple renal cysts and hypertension are associated with angiotensinogen (AGT) gene variant in Shiraz population (Iran).
abstract: OBJECTIVE
  To our knowledge, the relationship between simple renal cysts, hypertension and three significant genes of the renin-angiotensin system (AGT, AT1R and ACE1) has not been studied. The present study was designed to search for possible relationships between these significant polymorphic components, hypertension and simple renal cysts in Shiraz province (Iran).
  
  
  METHODS
  A total of 160 participants were recruited from the Motahari Clinic at Shiraz University of Medical Sciences. The subjects were divided into four main groups. Detection of the ACE1 genotype was performed with a nested-polymerase chain reaction (PCR) protocol. Two separate restriction fragment length polymorphism-PCR assays were used to identify AGT and AT1R genotypes.
  
  
  RESULTS
  The allele frequency of AGT M235T differed significantly between group 1 (patients with simple renal cysts and hypertension) and normal individuals (p < 0.05). There were no significant differences in frequency for the other genes (ACE1 and AT1R).
  
  
  CONCLUSIONS
  Our findings show a relationship between the AGT-TT genotype and hypertension in patients with both hypertension and simple renal cysts. This finding suggests an additive role for the AGT gene of the renin-angiotensin system in the process of hypertension and simple renal cysts formation. Future studies are needed to elucidate the mechanisms through which this association is mediated.
authors.affiliation: Department of Medical Genetics tabeismb@sums.ac.ir.
authors.forename: S M B
authors.initials: SM
authors.lastname: Tabei
authors.affiliation: Department of Medical Genetics.
authors.forename: A
authors.initials: A
authors.lastname: Nariman
authors.affiliation: Department of Medical Genetics.
authors.forename: K
authors.initials: K
authors.lastname: Daliri
authors.affiliation: Shiraz Nephrology-Urology Research Center.
authors.forename: J
authors.initials: J
authors.lastname: Roozbeh
authors.affiliation: Department of Urology.
authors.forename: A
authors.initials: A
authors.lastname: Khezri
authors.affiliation: Department of Medical Genetics.
authors.forename: H R
authors.initials: HR
authors.lastname: Goodarzi
authors.affiliation: Department of Radiology, Shiraz University of Medical Sciences, Iran.
authors.forename: M
authors.initials: M
authors.lastname: Lotfi
authors.affiliation: Department of Radiology, Shiraz University of Medical Sciences, Iran.
authors.forename: S
authors.initials: S
authors.lastname: Sefidbakht
authors.affiliation: Department of Medical Genetics.
authors.forename: M
authors.initials: M
authors.lastname: Entezam
mesh_terms: D000808:Angiotensinogen / Q000235:genetics*; D004587:Electrophoresis, Agar Gel; D005006:Ethnicity / Q000235:genetics*; D005787:Gene Frequency / Q000235:genetics; D020022:Genetic Predisposition to Disease; D006801:Humans; D006973:Hypertension / Q000150:complications / Q000235:genetics*; D007492:Iran; D052177:Kidney Diseases, Cystic / Q000150:complications / Q000235:genetics*; D008875:Middle Aged; D007703:Peptidyl-Dipeptidase A / Q000235:genetics; D011110:Polymorphism, Genetic; D044140:Receptor, Angiotensin, Type 1 / Q000235:genetics
chemical_list: D044140:Receptor, Angiotensin, Type 1; D000808:Angiotensinogen; C488034:ACE protein, human; D007703:Peptidyl-Dipeptidase A
keywords: Hypertension; angiotensinogen; polymorphism; renin-angiotensin system; simple renal cyst