title: Conditioned medium of Wnt/β-catenin signaling-activated olfactory ensheathing cells promotes synaptogenesis and neurite growth in vitro.
abstract: Olfactory ensheathing cells (OECs), the major glia cells in the olfactory system, have been extensively studied because of their ability to promote axonal growth and regeneration. Whether it could facilitate synaptogenesis is an important, but remains as yet an unanswered question. We have identified a subgroup of Wnt signaling-activated OECs, spatiotemporal distribution of which in the olfactory bulb suggests a role for these cells in both axonal growth and synaptogenesis. In the present study, we explored this possibility in vitro. OECs were primarily cultured, in which Wnt signaling was activated by overexpressing β-catenin, and inhibited by dominant negative TCF4. Neurite growth and synaptogenesis were assessed by co-culturing neurons with conditioned medium from control OECs (cOECs CM), Wnt/β-catenin signaling-activated OECs (wOECs CM), or Wnt signaling-inhibited OECs (wiOECs). The results showed that although cOECs CM enhances axonal growth, wOECs CM exhibited a stronger axonal growth-promoting effect, than cOECs CM. More importantly, wOECs CM stimulates synatpogenesis, demonstrated by the expression of Synaptophysin and whole-cell patch clamp recording. In contrast, both cOECs CM and wiOECs CM do not affect synaptogenesis. Our data, for the first time, demonstrated that, in comparison with regularly cultured OECs, wOECs CM are more effective in enhancing axonal growth, and can promote synaptogenesis, probably by secreting factors. These results suggest a potential application of wOECs for treating spinal cord injury.
authors.affiliation: Department of Neurology, Xijing Hospital, Fourth Military Medical University, 127 Chang Le Xi Road, Xi'an, 710032, Shaanxi, China.
authors.forename: Zhenyu
authors.initials: Z
authors.lastname: Yang
authors.forename: Yin
authors.initials: Y
authors.lastname: Wu
authors.forename: Lianhe
authors.initials: L
authors.lastname: Zheng
authors.forename: Chen
authors.initials: C
authors.lastname: Zhang
authors.forename: Jialei
authors.initials: J
authors.lastname: Yang
authors.forename: Ming
authors.initials: M
authors.lastname: Shi
authors.forename: Dongyun
authors.initials: D
authors.lastname: Feng
authors.forename: Zhongliang
authors.initials: Z
authors.lastname: Wu
authors.forename: Ya-Zhou
authors.initials: YZ
authors.lastname: Wang
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D018920:Coculture Techniques; D017077:Culture Media, Conditioned / Q000494:pharmacology*; D051379:Mice; D008810:Mice, Inbred C57BL; D016501:Neurites / Q000187:drug effects / Q000378:metabolism*; D009830:Olfactory Bulb / Q000166:cytology*; D010641:Phenotype; D013569:Synapses / Q000187:drug effects / Q000378:metabolism*; D060449:Wnt Signaling Pathway / Q000187:drug effects*
chemical_list: D017077:Culture Media, Conditioned
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