title: Molecular markers of mucosa harboring gastric adenomas.
abstract: Gastric adenoma is a precursor lesion of the adenocarcinoma.
  
  
  
  To characterize gastric adenomas according to the mucin immunoexpression and to evaluate the immunoexpression of p53, p16ink4a, BCL-2, cyclin D, Ki-67, in the adenoma and in the gastric mucosa harboring adenoma.
  
  
  
  Forty gastric specimens from 20 patients were classified as intestinal (MUC2-goblet cell mucin) or foveolar (MUC5AC-gastric-foveolar mucin) adenomas. Immunohistochemistry was performed using streptavidin-biotin-complex method.
  
  
  
  Twelve (60%) patients were men. The mean age was 67.9±12.9 years-old. Intestinal adenomas were detected in 13 (65%) patients and gastric type in 7 (35%). Low-grade dysplasia was present in 13 (65%) of the adenomas, high-grade in 3 (15%), and adenocarcinoma within the polyp in 4 (20%). Six (30%) patients had synchronous adenocarcinoma. p53 immunoexpression was observed in 6/20 (30%) of adenomas, and in 2/6 (33.3%) of synchronous tumors. There was an association between p53 immunoexpression and intestinal type of adenoma/tumor, P=0.04. There was no association between p16ink4a, Bcl-2, cyclin D and Ki-67 and adenoma clinicopathological characteristics.
  
  
  
  Immunohistochemistry may be useful to classify the adenomas subtypes and may define the pathway of adenoma to carcinoma sequence.
authors.affiliation: Departamento de Gastroenterologia, Faculdade de Medicina da Universidade de São Paulo, São Paulo, SP, Brazil. adrisafatleribeiro@terra.com.br
authors.forename: Adriana Vaz
authors.initials: AV
authors.lastname: Safatle-Ribeiro
authors.forename: Kátia Adriana Tessima
authors.initials: KA
authors.lastname: Franco
authors.forename: Carlos Eduardo Pereira
authors.initials: CE
authors.lastname: Corbett
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Iriya
authors.forename: Bruno
authors.initials: B
authors.lastname: Zilberstein
authors.forename: Ulysses
authors.initials: U
authors.lastname: Ribeiro
mesh_terms: D000236:Adenoma / Q000378:metabolism* / Q000473:pathology; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000097:blood*; D056741:Cyclin D / Q000097:blood; D019941:Cyclin-Dependent Kinase Inhibitor p16 / Q000097:blood; D005260:Female; D005753:Gastric Mucosa / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D016130:Immunophenotyping; D019394:Ki-67 Antigen / Q000097:blood; D008297:Male; D008875:Middle Aged; D009077:Mucins / Q000378:metabolism*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000097:blood; D013274:Stomach Neoplasms / Q000378:metabolism* / Q000473:pathology; D016159:Tumor Suppressor Protein p53 / Q000097:blood
chemical_list: D014408:Biomarkers, Tumor; D056741:Cyclin D; D019941:Cyclin-Dependent Kinase Inhibitor p16; D019394:Ki-67 Antigen; D009077:Mucins; D019253:Proto-Oncogene Proteins c-bcl-2; D016159:Tumor Suppressor Protein p53