title: Outward stabilization of the voltage sensor in domain II but not domain I speeds inactivation of voltage-gated sodium channels.
abstract: To determine the roles of the individual S4 segments in domains I and II to activation and inactivation kinetics of sodium current (INa) in NaV1.5, we used a tethered biotin and avidin approach after a site-directed cysteine substitution was made in the second outermost Arg in each S4 (DI-R2C and DII-R2C). We first determined the fraction of gating charge contributed by the individual S4's to maximal gating current (Qmax), and found that the outermost Arg residue in each S4 contributed ∼19% to Qmax with minimal contributions by other arginines. Stabilization of the S4's in DI-R2C and DII-R2C was confirmed by measuring the expected reduction in Qmax. In DI-R2C, stabilization resulted in a decrease in peak INa of ∼45%, while its peak current-voltage (I-V) and voltage-dependent Na channel availability (SSI) curves were nearly unchanged from wild type (WT). In contrast, stabilization of the DII-R2C enhanced activation with a negative shift in the peak I-V relationship by -7 mV and a larger -17 mV shift in the voltage-dependent SSI curve. Furthermore, its INa decay time constants and time-to-peak INa became more rapid than WT. An explanation for these results is that the depolarized conformation of DII-S4, but not DI-S4, affects the receptor for the inactivation particle formed by the interdomain linker between DIII and IV. In addition, the leftward shifts of both activation and inactivation and the decrease in Gmax after stabilization of the DII-S4 support previous studies that showed β-scorpion toxins trap the voltage sensor of DII in an activated conformation.
authors.affiliation: The Nora Eccles Harrison Cardiovascular Research and Training Institute and the Department of Internal Medicine, University of Utah, Salt Lake City, Utah; and.
authors.forename: Michael F
authors.initials: MF
authors.lastname: Sheets
authors.forename: Tiehua
authors.initials: T
authors.lastname: Chen
authors.forename: Dorothy A
authors.initials: DA
authors.lastname: Hanck
mesh_terms: D001120:Arginine; D006801:Humans; D008564:Membrane Potentials / Q000502:physiology*; D062554:NAV1.5 Voltage-Gated Sodium Channel / Q000502:physiology*; D018408:Patch-Clamp Techniques; D017434:Protein Structure, Tertiary / Q000502:physiology*
chemical_list: D062554:NAV1.5 Voltage-Gated Sodium Channel; C568320:SCN5A protein, human; D001120:Arginine
keywords: gating charge; inactivation; sodium channel; stabilization; voltage sensor; β-scorpion toxin