title: Structure of a Kdo-containing O polysaccharide representing Proteus O79, a newly described serogroup for some clinical Proteus genomospecies isolates from Poland.
abstract: From 41 Proteus genomospecies strains isolated in Poland, seven displayed similar serospecificity in ELISA with intact and adsorbed O antisera as well as in Western blot. The cross-reacting strains were found to belong to Proteus genomospecies 5/6 and classified into a new Proteus serogroup, O79, which seems to be widespread among Proteus genomospecies clinical isolates in Lodz, Poland. The O polysaccharide of the lipopolysaccharide of a representative O79 strain, 11 B-r, was studied by chemical analyses and (1)H and (13)C NMR spectroscopy, and the following structure of the repeating unit was established: →4)-α-D-GlcpNAlaAc-(1→5)-α-Kdop-(2→2)-α-D-Glcp-(1→3)-β-D-GlcpNAc-(1→ where AlaAc indicates N-acetyl-L-alanyl and Kdo indicates 3-deoxy-D-manno-oct-2-ulosonic acid. The O polysaccharide was unstable under mild acidic conditions and cleaved by acid-labile linkages of Kdo residues to yield a tetrasaccharide with Kdo at the reducing end. The structure established is unique among Proteus O polysaccharides, which is in agreement with the lack of any significant cross-reactivity for the lipopolysaccharide of strain 11 B-r and O antisera against strains of all known Proteus O serogroups and vice versa.
authors.affiliation: ND Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russian Federation.
authors.forename: Nikolay P
authors.initials: NP
authors.lastname: Arbatsky
authors.forename: Dominika
authors.initials: D
authors.lastname: Drzewiecka
authors.forename: Agata
authors.initials: A
authors.lastname: Palusiak
authors.forename: Alexander S
authors.initials: AS
authors.lastname: Shashkov
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Zabłotni
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Siwińska
authors.forename: Yuriy A
authors.initials: YA
authors.lastname: Knirel
mesh_terms: D000143:Acids / Q000737:chemistry; D002236:Carbohydrate Conformation; D009682:Magnetic Resonance Spectroscopy; D019081:O Antigens / Q000737:chemistry* / Q000302:isolation & purification; D011044:Poland; D011511:Proteus / Q000737:chemistry* / Q000145:classification* / Q000302:isolation & purification
chemical_list: D000143:Acids; D019081:O Antigens
keywords: 3-Deoxy-d-manno-oct-2-ulosonic acid; Bacterial polysaccharide structure; Lipopolysaccharide; O antigen; Proteus Genomospecies; Serological classification