title: A poly(A)-specific ribonuclease directly regulates the poly(A) status of mitochondrial mRNA in Arabidopsis.
abstract: Coordination of gene expression in the organelles and the nucleus is important for eukaryotic cell function. Transcriptional and post-transcriptional gene regulation in mitochondria remains incompletely understood in most eukaryotes, including plants. Here we show that poly(A)-specific ribonuclease, which influences the poly(A) status of cytoplasmic mRNA in many eukaryotes, directly regulates the poly(A) tract of mitochondrial mRNA in conjunction with a bacterial-type poly(A) polymerase, AGS1, in Arabidopsis. An Arabidopsis poly(A)-specific ribonuclease-deficient mutant, ahg2-1, accumulates polyadenylated mitochondrial mRNA and shows defects in mitochondrial protein complex levels. Mutations of AGS1 suppress the ahg2-1 phenotype. Mitochondrial localizations of AHG2 and AGS1 are required for their functions in the regulation of the poly(A) tract of mitochondrial mRNA. Our findings suggest that AHG2 and AGS1 constitute a regulatory system that controls mitochondrial mRNA poly(A) status in Arabidopsis.
authors.affiliation: Institute of Plant Science and Resources, Okayama University, 2-20-1 Chuo, Kurashiki, Okayama 710-0046, Japan. hira-t@okayama-u.ac.jp
authors.forename: Takashi
authors.initials: T
authors.lastname: Hirayama
authors.forename: Takakazu
authors.initials: T
authors.lastname: Matsuura
authors.forename: Sho
authors.initials: S
authors.lastname: Ushiyama
authors.forename: Mari
authors.initials: M
authors.lastname: Narusaka
authors.forename: Yukio
authors.initials: Y
authors.lastname: Kurihara
authors.forename: Michiko
authors.initials: M
authors.lastname: Yasuda
authors.forename: Misato
authors.initials: M
authors.lastname: Ohtani
authors.forename: Motoaki
authors.initials: M
authors.lastname: Seki
authors.forename: Taku
authors.initials: T
authors.lastname: Demura
authors.forename: Hideo
authors.initials: H
authors.lastname: Nakashita
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Narusaka
authors.forename: Shimpei
authors.initials: S
authors.lastname: Hayashi
mesh_terms: D017360:Arabidopsis / Q000201:enzymology* / Q000235:genetics*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D005095:Exoribonucleases / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant; D016153:Genes, Suppressor; D049452:Green Fluorescent Proteins / Q000378:metabolism; D008928:Mitochondria / Q000378:metabolism; D024101:Mitochondrial Proteins / Q000378:metabolism; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D010641:Phenotype; D018517:Plant Roots / Q000254:growth & development; D011061:Poly A / Q000378:metabolism*; D026723:Polyadenylation / Q000235:genetics; D021381:Protein Transport; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D000077278:RNA, Mitochondrial; D013489:Suppression, Genetic
chemical_list: D029681:Arabidopsis Proteins; D005095:Exoribonucleases; D049452:Green Fluorescent Proteins; D024101:Mitochondrial Proteins; D011061:Poly A; D012333:RNA, Messenger; D000077278:RNA, Mitochondrial; C029305:mitochondrial messenger RNA