title: Bovine pulp tissue dissolution ability of HealOzone®, Aquatine Alpha Electrolyte® and sodium hypochlorite.
abstract: The aim of this study was to evaluate the bovine pulp tissue dissolution ability of HealOzone, Aquatine Alpha Electrolyte® and 0.5% sodium hypochlorite, used alone or in combination. Thirty bovine pulp fragments were weighed, divided into six groups and placed individually in Eppendorf tubes containing the tested solution until total dissolution occurred. The groups were: G1: saline (negative control), G2: Aquatine Alpha Electrolyte®, G3: 0.5% NaOCl (positive control), G4: Saline + HealOzone, G5: 0.5% NaOCl + HealOzone, G6: Aquatine Alpha Electrolyte® + HealOzone. HealOzone was activated for 2 min with a #6 cup covering the test tube opening on a fixed platform. Two blinded observers using 2× loupes magnification assessed the samples continuously for the first 2 h, and then every hour for the next 8 h. Dissolution speed was calculated by dividing pulp weight by dissolution time (mg min(-1) ). G3 (NaOCl) and G5 (NaOCl + HealOzone) dissolved the pulp tissue completely. The mean dissolution speed for G3 was 0.396 mg min(-1) (SD 0.032) and for G5 was 0.775 mg min(-1) (SD 0.2). Student's t-test showed that G5 dissolved bovine pulp tissue faster than G3 (P = 0.01). Only groups containing sodium hypochlorite dissolved pulp tissue, whilst HealOzone enhanced speed of dissolution.
authors.affiliation: Postgraduate Dental Education Unit, Institute of Clinical Education, Warwick Medical School, University of Warwick, Coventry, UK. grossifede@yahoo.com
authors.forename: Giampiero
authors.initials: G
authors.lastname: Rossi-Fedele
authors.forename: Liviu
authors.initials: L
authors.lastname: Steier
authors.forename: Esma Jane
authors.initials: EJ
authors.lastname: Dogramaci
authors.forename: Luigi
authors.initials: L
authors.lastname: Canullo
authors.forename: Gabriela
authors.initials: G
authors.lastname: Steier
authors.forename: Jose Antonio Poli
authors.initials: JA
authors.lastname: de Figueiredo
mesh_terms: D000818:Animals; D002417:Cattle; D003782:Dental Pulp / Q000187:drug effects*; D004338:Drug Combinations; D055664:Electrochemical Techniques; D006861:Hydrogen Peroxide / Q000494:pharmacology; D006997:Hypochlorous Acid / Q000494:pharmacology*; D016877:Oxidants / Q000494:pharmacology; D010126:Ozone / Q000494:pharmacology*; D012388:Root Canal Irrigants / Q000494:pharmacology*; D012965:Sodium Chloride; D012973:Sodium Hypochlorite / Q000494:pharmacology*; D012995:Solubility; D013997:Time Factors
chemical_list: D004338:Drug Combinations; D016877:Oxidants; D012388:Root Canal Irrigants; C118085:superoxidized water; D012965:Sodium Chloride; D010126:Ozone; D006997:Hypochlorous Acid; D006861:Hydrogen Peroxide; D012973:Sodium Hypochlorite
keywords: dental pulp; ozone; sodium hypochlorite; superoxidised water; tissue dissolution