title: Cellular interactions of doxorubicin-loaded DNA-modified halloysite nanotubes.
abstract: Halloysite nanotube (HNT)-based supramolecular complexes are synthesized and evaluated with respect to their cytotoxicity and effects on cellular structures. As HNTs are water-insoluble, DNA is applied for wrapping the surface of HNTs to enhance their water-dispersibility. To investigate the potential of DNA-wrapped HNTs (HD) as a promising drug delivery carrier, doxorubicin (DOX) is introduced as a model anticancer agent and loaded onto HD. The DOX-loaded, DNA-wrapped HNTs (HDD) show sustained DOX release over two weeks without initial burst of DOX indicating delayed DOX release inside cells. In addition, effects of DNA-wrapped HNTs (HD) or HDD on the cytoskeleton organization of A549 cells are studied by visualizing the distribution of F-actin filaments using confocal laser scanning microscopy, and cellular morphological changes are observed by scanning electron microscopy and scanning ion conductance microscopy.
authors.affiliation: Physikalisches Institut, Universität Münster, Wilhelm Klemm-Str. 10, 48149 Münster, Germany.
authors.forename: Yeonju
authors.initials: Y
authors.lastname: Lee
authors.forename: Goo-Eun
authors.initials: GE
authors.lastname: Jung
authors.forename: Sang Joon
authors.initials: SJ
authors.lastname: Cho
authors.forename: Kurt E
authors.initials: KE
authors.lastname: Geckeler
authors.forename: Harald
authors.initials: H
authors.lastname: Fuchs
mesh_terms: D000199:Actins / Q000378:metabolism; D000538:Aluminum Silicates / Q000737:chemistry*; D000970:Antineoplastic Agents / Q000737:chemistry* / Q000378:metabolism; D045744:Cell Line, Tumor; D000077215:Clay; D004247:DNA / Q000737:chemistry*; D004317:Doxorubicin / Q000737:chemistry* / Q000378:metabolism; D004337:Drug Carriers / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D006863:Hydrogen-Ion Concentration; D018613:Microscopy, Confocal; D043942:Nanotubes / Q000737:chemistry*
chemical_list: D000199:Actins; D000538:Aluminum Silicates; D000970:Antineoplastic Agents; D004337:Drug Carriers; D005456:Fluorescent Dyes; D004317:Doxorubicin; D004247:DNA; D000077215:Clay