title: COMMD1 modulates noxious inflammation in cystic fibrosis.
abstract: Cystic fibrosis (CF) is an autosomal recessive disease caused by mutations in the cystic fibrosis transmembrane conductance regulator (CFTR) gene, which encodes an epithelial anion channel. Morbidity is mainly due to lung disease, which is characterized by chronic neutrophilic inflammation. Deregulation of inflammatory pathways is observed in the airways of CF patients, as evidenced by exaggerated NF-κB activity, causing an increase in the local release of pro-inflammatory cytokines such as IL-8. COMMD1, a pleiotropic protein, was recently shown to interact with CFTR and to promote CFTR cell surface expression. The effect of COMMD1 on the NF-κB pathway was assessed in CF and non-CF bronchial epithelial cells by knockdown and overexpression experiments. Results showed that (i) COMMD1 knockdown induced NF-κB-dependent transcription, (ii) COMMD1 overexpression inhibited NF-κB activity and was associated with a decrease in IL-8 transcript level and protein secretion. These data demonstrate the anti-inflammatory properties of COMMD1 in bronchial epithelial cells and open new therapeutic avenues in CF.
authors.affiliation: INSERM U955, Créteil 94010, France; Université Paris-Est, Créteil 94010, France; AP-HP, CHU H. Mondor, Pôle de Biologie, Créteil 94010, France.
authors.forename: Alix
authors.initials: A
authors.lastname: de Becdelièvre
authors.forename: Jérémy
authors.initials: J
authors.lastname: Rocca
authors.forename: Abdel
authors.initials: A
authors.lastname: Aissat
authors.forename: Loic
authors.initials: L
authors.lastname: Drévillon
authors.forename: Stéphane
authors.initials: S
authors.lastname: Moutereau
authors.forename: Sabine
authors.initials: S
authors.lastname: Le Gouvello
authors.forename: Alexandre
authors.initials: A
authors.lastname: Hinzpeter
authors.forename: Agathe
authors.initials: A
authors.lastname: Tarze
authors.forename: Pascale
authors.initials: P
authors.lastname: Fanen
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000378:metabolism*; D001980:Bronchi / Q000473:pathology; D002460:Cell Line; D003550:Cystic Fibrosis / Q000150:complications* / Q000378:metabolism* / Q000473:pathology; D015536:Down-Regulation; D004847:Epithelial Cells / Q000378:metabolism; D006801:Humans; D007249:Inflammation / Q000150:complications* / Q000378:metabolism* / Q000473:pathology; D016209:Interleukin-8 / Q000235:genetics / Q000378:metabolism; D008954:Models, Biological; D016328:NF-kappa B / Q000378:metabolism; D011401:Promoter Regions, Genetic / Q000235:genetics; D018808:Transcription Factor AP-1 / Q000378:metabolism; D014158:Transcription, Genetic
chemical_list: D048868:Adaptor Proteins, Signal Transducing; C480519:COMMD1 protein, human; D016209:Interleukin-8; D016328:NF-kappa B; D018808:Transcription Factor AP-1
keywords: CFTR; COMMD1; IL-8; Inflammation; NF-kappaB