title: Simultaneous detection of SERS and fluorescence using a single excitation for microbead-based analysis.
abstract: We demonstrate simultaneous detection of surface-enhanced Raman scattering (SERS) and fluorescence signals from a silver microbead. For the dual signal generation, silver microbeads with a diameter of 15 microm were functionalized with benzenethiol (BT) as a Raman tag and a cardiac troponin I (cTnI) antibody on their surface. SERS and fluorescence signals were obtained using a single argon laser source with 488 nm wavelength. The SERS signals from Raman tag can be used as identification indices for decoding a particular microbead, while the fluorescence signals provide the information about molecular interactions with a specific biomarker. With simultaneous detection of SERS and fluorescence signals using single excitation on the functionalized microbeads, we successfully showed the possibility of a simple barcoding strategy for multiplex analysis using suspension arrays.
authors.affiliation: Interdisciplinary Program Biomedical Engineering Major, Seoul National University, 28 Yongon-dong, Chongno-gu, Seoul, Korea.
authors.forename: Sa Ram
authors.initials: SR
authors.lastname: Lee
authors.forename: Chang Su
authors.initials: CS
authors.lastname: Jeon
authors.forename: Inseong
authors.initials: I
authors.lastname: Hwang
authors.forename: Taek Dong
authors.initials: TD
authors.lastname: Chung
authors.forename: Hee Chan
authors.initials: HC
authors.lastname: Kim
mesh_terms: D008863:Microspheres; D057054:Molecular Imaging / Q000379:methods*; D015336:Molecular Probe Techniques; D012834:Silver / Q000032:analysis* / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013059:Spectrum Analysis, Raman / Q000379:methods*
chemical_list: D012834:Silver