title: Bilirubin oxidases in bioelectrochemistry: features and recent findings.
abstract: Bilirubin oxidases, a sub class of the Multicopper oxidases family, were discovered in 1981 by Tanaka and Murao (Murao and Tanaka, 1981) and first used for the detection of bilirubin. Since 2001 and the pioneering work of Tsujimura, these BODs have attracted a lot of attention for the reduction of O2. Unlike laccases, these BODs are stable in physiological conditions (20mM phosphate buffer, pH 7.4, 0.14 M NaCl, 37 °C) and more than 120 papers have been published in the last 7 years. Here, we will first briefly describe some general features of BODs and then review the use of BODs for bilirubin biosensors and the recent achievements and progress toward the elaboration of efficient O2 reducing cathodes.
authors.affiliation: CNRS, CRPP, UPR 8641, Pessac F-33600, France; University of Bordeaux, CRPP, UPR 8641, Pessac F-33600, France. Electronic address: mano@crpp-bordeaux.cnrs.fr.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Mano
authors.forename: Lise
authors.initials: L
authors.lastname: Edembe
mesh_terms: D000818:Animals; D001419:Bacteria / Q000201:enzymology*; D001663:Bilirubin / Q000032:analysis* / Q000097:blood; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D055664:Electrochemical Techniques / Q000295:instrumentation / Q000379:methods; D005658:Fungi / Q000201:enzymology*; D006801:Humans; D008958:Models, Molecular; D044925:Oxidoreductases Acting on CH-CH Group Donors / Q000737:chemistry / Q000378:metabolism*
chemical_list: D044925:Oxidoreductases Acting on CH-CH Group Donors; C042401:bilirubin oxidase; D001663:Bilirubin
keywords: 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid); ABTS; BFC; BOD; BSA; Bilirubin biosensors; Bilirubin oxidases; Biofuel cells; DET; Direct electron transfer; EPR; GOx; Gln; HOPG; MCO; MET; MWNT; Mediated electron transfer; Met; Mv-BOD; NHE; Oxygen reduction; PGE; PTFE; SCE; SECM; SPGE; SWNT; Tt-BOD; bilirubin oxidase; bilirubin oxidase from Myrothecium verrucaria; bilirubin oxidase from Trachyderma tsunodae; biofuel cell; bovine serum albumin; direct electron transfer; electron paramagnetic resonance spectroscopy; glucose oxidase; glutamine; highly oriented pyrolytic graphite; mediated electron transfer; methionine; multicopper oxidase; multiwall carbon nanotubes; normal hydrogen electrode; poly(tetrafluoroethylene); pyrolytic graphite electrode; saturated calomel electrode; scanning electrochemical microscopy; single wall nanotubes; spectroscopic graphite