title: Plasmodium falciparum merozoite surface antigen, PfRH5, elicits detectable levels of invasion-inhibiting antibodies in humans.
abstract: Plasmodium falciparum is an intracellular protozoan parasite that infects erythrocytes and hepatocytes. The blood stage of its life cycle causes substantial morbidity and mortality associated with millions of infections each year, motivating an intensive search for potential components of a multi-subunit vaccine. In this study, we present data showing that antibodies from natural infections can recognize a recombinant form of the relatively conserved merozoite surface antigen, PfRH5. Furthermore, we performed invasion inhibition assays on clinical isolates and laboratory strains of P. falciparum in the presence of affinity purified antibodies to RH5 and show that these antibodies can inhibit invasion in vitro.
authors.affiliation: Harvard School of Public Health.
authors.forename: Saurabh D
authors.initials: SD
authors.lastname: Patel
authors.forename: Ambroise D
authors.initials: AD
authors.lastname: Ahouidi
authors.forename: Amy K
authors.initials: AK
authors.lastname: Bei
authors.forename: Tandakha N
authors.initials: TN
authors.lastname: Dieye
authors.forename: Souleymane
authors.initials: S
authors.lastname: Mboup
authors.forename: Stephen C
authors.initials: SC
authors.lastname: Harrison
authors.forename: Manoj T
authors.initials: MT
authors.lastname: Duraisingh
mesh_terms: D000913:Antibodies, Protozoan / Q000097:blood / Q000276:immunology*; D000953:Antigens, Protozoan / Q000737:chemistry / Q000276:immunology*; D002352:Carrier Proteins / Q000737:chemistry / Q000276:immunology*; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology; D016778:Malaria, Falciparum / Q000276:immunology*; D052940:Merozoites / Q000276:immunology*; D010963:Plasmodium falciparum / Q000276:immunology*; D011994:Recombinant Proteins / Q000737:chemistry / Q000276:immunology
chemical_list: D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D002352:Carrier Proteins; D007074:Immunoglobulin G; C531020:RH5 protein, Plasmodium falciparum; D011994:Recombinant Proteins
keywords: Plasmodium falciparum; RH2; RH5; invasion; malaria; recombinant protein expression; vaccine candidate
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