title: Evolution of Pseudomonas aeruginosa virulence as a result of phage predation.
abstract: The rapid increase in the emergence of antibiotic-resistant bacteria has attracted attention to bacteriophages for treating and preventing bacterial infections. Bacteriophages can drive the diversification of Pseudomonas aeruginosa, giving rise to phage-resistant variants with different phenotypes from their ancestral hosts. In this study, we sought to investigate the effect of phage resistance on cytotoxicity of host populations toward cultured mammalian cells. The library of phage-resistant P. aeruginosa PAO1 variants used was developed previously via experimental evolution of an isogenic host population using phages PP7 and E79. Our results presented herein indicate that the phage-resistant variants developed in a heterogeneous phage environment exhibit a greater ability to impede metabolic action of cultured human keratinocytes and have a greater tendency to cause membrane damage even though they cannot invade the cells in large numbers. They also show a heightened resistance to phagocytosis by model murine macrophages. Furthermore, all isolates produced higher levels of at least one of the secreted virulence factors, namely, total proteases, elastase, phospholipase C, and hemolysins. Reverse transcription-quantitative PCR (RT-qPCR) revealed upregulation in the transcription of a number of genes associated with virulence of P. aeruginosa for the phage-resistant variants. The results of this study indicate a significant change in the in vitro virulence of P. aeruginosa following phage predation and highlight the need for caution in the selection and design of phages and phage cocktails for therapeutic use.
authors.affiliation: Department of Chemical Engineering, McGill University, Montreal, Quebec, Canada.
authors.forename: Zeinab
authors.initials: Z
authors.lastname: Hosseinidoust
authors.forename: Theo G M
authors.initials: TG
authors.lastname: van de Ven
authors.forename: Nathalie
authors.initials: N
authors.lastname: Tufenkji
mesh_terms: D000818:Animals; D001435:Bacteriophages / Q000254:growth & development*; D002460:Cell Line; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial; D006801:Humans; D015603:Keratinocytes / Q000382:microbiology / Q000502:physiology; D008264:Macrophages / Q000276:immunology / Q000382:microbiology; D051379:Mice; D009154:Mutation; D010587:Phagocytosis; D011550:Pseudomonas aeruginosa / Q000235:genetics / Q000472:pathogenicity* / Q000821:virology*; D060888:Real-Time Polymerase Chain Reaction; D014774:Virulence; D037521:Virulence Factors / Q000378:metabolism*
chemical_list: D037521:Virulence Factors
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