title: Cloning and characterization of a FLO/LFY ortholog in Gossypium hirsutum L.
abstract: CONCLUSIONS
  GhLFY was cloned from G. hirsutum L. Its expression, subcellular localization, and function were analyzed, as well as the in vivo regulation of GhLFY by the MADS-box protein SOC1 (GhSOC1).
  Flowering is a very important phase during which plants produce the organs for sexual reproduction. The FLORICAULA/LEAFY (FLO/LFY) homologs play a major role in the initiation of flowering. To understand the mechanism of the transition from the vegetative to reproductive phases in Upland cotton (Gossypium hirsutum L.), we isolated a candidate LFY gene from G. hirsutum L. (GhLFY) that showed a high degree of similarity to other plant homologs of FLO/LFY. qPCR analysis showed that GhLFY was highly expressed in the shoot apex, with substantial upregulation at the third true leaf expansion stage during floral bud differentiation. Subcellular localization studies revealed GhLFY localization in the nucleus. Ectopic expression of the GhLFY coding region in Arabidopsis resulted in early flowering. The expression of the GhLFY coding region under the control of the 35S promoter complemented the lfy-5 mutation in transgenic Arabidopsis lfy-5 mutant plants. Furthermore, a chromatin immunoprecipitation assay revealed that GhLFY may function downstream of GhSOC1 during the initiation of flowering in G. hirsutum L. GhLFY was likely to be regulated by GhSOC1, which binds to the LFY promoter in Arabidopsis. These results suggest that GhLFY is a FLO/LFY ortholog that may be involved in controlling flowering time and floral development.
authors.affiliation: Key Laboratory of Cotton Genetic Improvement of Ministry of Agriculture, The Cotton Research Institute, Chinese Academy of Agricultural Sciences, Anyang, 455000, Henan, People's Republic of China.
authors.forename: Jie
authors.initials: J
authors.lastname: Li
authors.forename: Shu-Li
authors.initials: SL
authors.lastname: Fan
authors.forename: Mei-Zhen
authors.initials: MZ
authors.lastname: Song
authors.forename: Chao-You
authors.initials: CY
authors.lastname: Pang
authors.forename: Heng-Ling
authors.initials: HL
authors.lastname: Wei
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.forename: Jian-hui
authors.initials: JH
authors.lastname: Ma
authors.forename: Jiang-Hui
authors.initials: JH
authors.lastname: Wei
authors.forename: Jian-guo
authors.initials: JG
authors.lastname: Jing
authors.forename: Shu-Xun
authors.initials: SX
authors.lastname: Yu
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics / Q000502:physiology; D029681:Arabidopsis Proteins / Q000235:genetics; D002467:Cell Nucleus / Q000378:metabolism; D003001:Cloning, Molecular; D035264:Flowers / Q000502:physiology; D018506:Gene Expression Regulation, Plant; D005816:Genetic Complementation Test; D003368:Gossypium / Q000235:genetics*; D049452:Green Fluorescent Proteins / Q000378:metabolism; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D017440:Photoperiod; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic / Q000235:genetics; D011485:Protein Binding; D021381:Protein Transport; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D013347:Subcellular Fractions / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics
chemical_list: D029681:Arabidopsis Proteins; C074747:LFY protein, Arabidopsis; D010940:Plant Proteins; D011993:Recombinant Fusion Proteins; D014157:Transcription Factors; D049452:Green Fluorescent Proteins
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