title: Effects of cystamine on antioxidant activities and regulatory T cells in lupus-prone mice.
abstract: Attenuated antioxidant activities, irregular cytokines expressions and reduced regulatory T cells, are strongly associated with the pathogenesis of systemic lupus erythematosus (SLE). Despite the well-established beneficial effects of cystamine on lupus-prone mice, the extent to which cystamine contributes to antioxidant activity and the reduction of regulatory T cells has seldom been investigated. Therefore, this study elucidates how cystamine affects anti-oxidant activities in NZB/W F1 mice by performing assays of Glutathione (GSH), 1,1-diphenyl-2- picryl-hydrazyl (DPPH) and malondialdehyde thiobarbituric acid (MDA). In addition, investigations of the effects of cystamine on CD4(+) /CD25(+) regulatory T cells and interleukin-6 (IL6)/STAT-3 signalling were performed with flow cytometry and immunoblots. Experimental results reveal more significantly reduced MDA and increased GSH and DPPH in NZB/W F1 mice receiving cystamine than in those mice receiving PBS. Meanwhile, CD4(+) /CD25(+) regulatory T cells more significantly increase in NZB/W F1 mice receiving cystamine than in those mice receiving PBS, accompanied by significantly reduced IL-6/phosphorylated STAT-3 expression. The above findings suggest the beneficial effects of cystamine in terms of increasing antioxidant activities and CD4(+) /CD25(+) regulatory T cells in lupus-prone mice by suppressing IL-6/STAT3 signalling.
authors.affiliation: Institute of Microbiology and Immunology, Chung Shan Medical University, Taichung, Taiwan; Clinical Laboratory, Chung Shan Medical University Hospital, Taichung, Taiwan; Graduate Institute of Basic Medical Science, China Medical University, Taichung, Taiwan; Department of Healthcare Administration, Asia University, Taichung, Taiwan.
authors.forename: Tsai-Ching
authors.initials: TC
authors.lastname: Hsu
authors.forename: Chun-Ching
authors.initials: CC
authors.lastname: Chiu
authors.forename: Yi-Wen
authors.initials: YW
authors.lastname: Wang
authors.forename: Bor-Show
authors.initials: BS
authors.lastname: Tzang
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D003538:Cystamine / Q000494:pharmacology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005434:Flow Cytometry; D005978:Glutathione / Q000378:metabolism; D008180:Lupus Erythematosus, Systemic / Q000473:pathology*; D051379:Mice; D050378:T-Lymphocytes, Regulatory / Q000187:drug effects*; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
chemical_list: D000975:Antioxidants; D017392:Thiobarbituric Acid Reactive Substances; D005978:Glutathione; D003538:Cystamine
keywords: Cystamine; Systemic lupus erythematosus (SLE); antioxidant; regulatory T cells
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