title: Evaluation of YadC protein delivered by live attenuated Salmonella as a vaccine against plague.
abstract: Yersinia pestis YadB and YadC are two new outer membrane proteins related to its pathogenicity. Here, codon-optimized yadC, yadC810 (aa 32-551), or yadBC antigen genes delivered by live attenuated Salmonella strains are evaluated in mice for induction of protective immune responses against Y. pestis CO92 through subcutaneous or intranasal challenge. Our findings indicate that mice immunized with Salmonella synthesizing YadC, YadC810, or YadBC develop significant serum IgG responses to purified recombinant YadC protein. For subcutaneous challenge (approximately 230 LD50 of Y. pestis CO92), mice immunized with Salmonella synthesizing YadC or YadC810 are afforded 50% protection, but no protection by immunization with the Salmonella strain synthesizing YadBC. None of these antigens provided protection against intranasal challenge (approximately 31 LD50 of Y. pestis CO92). In addition, subcutaneous immunization with purified YadC810 protein emulsified with alum adjuvant does not elicit a protective response against Y. pestis administered by either challenge route.
authors.affiliation: Center for Infectious Disease and Vaccinology, The Biodesign Institute, Arizona State University, Tempe, AZ, USA.
authors.forename: Wei
authors.initials: W
authors.lastname: Sun
authors.forename: Joseph
authors.initials: J
authors.lastname: Olinzock
authors.forename: Shifeng
authors.initials: S
authors.lastname: Wang
authors.forename: Shilpa
authors.initials: S
authors.lastname: Sanapala
authors.forename: Roy
authors.initials: R
authors.lastname: Curtiss
mesh_terms: D018829:Adhesins, Bacterial / Q000235:genetics / Q000276:immunology*; D000281:Administration, Intranasal; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D004195:Disease Models, Animal; D004337:Drug Carriers / Q000008:administration & dosage; D005260:Female; D007279:Injections, Subcutaneous; D008807:Mice, Inbred BALB C; D010930:Plague / Q000517:prevention & control*; D010931:Plague Vaccine / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D012475:Salmonella / Q000235:genetics / Q000254:growth & development; D016019:Survival Analysis; D014613:Vaccines, Attenuated / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology
chemical_list: D018829:Adhesins, Bacterial; D000907:Antibodies, Bacterial; D004337:Drug Carriers; D010931:Plague Vaccine; D014613:Vaccines, Attenuated; D014614:Vaccines, Synthetic; C526542:YadC protein, Yersinia pestis
keywords: Protective response; YadC; Yersinia pestis; bubonic plague; live attenuated Salmonella vaccine; pneumonic plague
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