title: Effectiveness of probiotic Phaeobacter bacteria grown in biofilters against Vibrio anguillarum infections in the rearing of Turbot (Psetta maxima) larvae.
abstract: The rearing environment of first-feeding turbot larvae, usually with high larvae densities and organic matter concentrations, may promote the growth of opportunistic pathogenic Vibrionaceae bacteria, compromising the survival of the larvae. The aim of this study was to assess the effectiveness of the biofilm-forming probiotic Phaeobacter 27-4 strain grown on a ceramic biofilter (probiofilter) in preventing Vibrio anguillarum infections in turbot larvae. In seawater with added microalgae and maintained under turbot larvae rearing conditions, the probiofilter reduced the total Vibrionaceae count and the concentration of V. anguillarum, which was undetectable after 144 h by real-time PCR. The probiofilter also improved the survival of larvae challenged with V. anguillarum, showing an accumulated mortality similar to that of uninfected larvae (35-40 %) and significantly (p < 0.05) lower than that of infected larvae with no probiofilter (76 %) due to a decrease in the pathogen concentration and in total Vibrionaceae. Furthermore, the probiofilter improved seawater quality by decreasing turbidity. Phaeobacter 27-4 released from the probiofilters was able to survive in the seawater for at least 11 days. The bacterial diversity in the larvae, analysed by denaturing gradient gel electrophoresis, was low, as in the live prey (rotifers), and remained unchanged in the presence of V. anguillarum or the probiofilter; however, the probiofilter reduced the bacterial carrying capacity of the seawater in the tanks. Phaeobacter-grown biofilters can constantly inoculate probiotics into rearing tanks and are therefore potentially useful for bacterial control in both open and recirculating industrial units.
authors.affiliation: Instituto de Investigacións Mariñas (IIM-CSIC), Eduardo Cabello 6, 36208, Vigo, Spain, majga@bio.dtu.dk.
authors.forename: María J
authors.initials: MJ
authors.lastname: Prol-García
authors.forename: José
authors.initials: J
authors.lastname: Pintado
mesh_terms: D000818:Animals; D017756:Aquaculture / Q000379:methods*; D002516:Ceramics / Q000627:therapeutic use; D058645:Denaturing Gradient Gel Electrophoresis; D005374:Filtration / Q000295:instrumentation / Q000379:methods; D005393:Fish Diseases / Q000382:microbiology* / Q000517:prevention & control*; D005412:Flatfishes; D007814:Larva / Q000382:microbiology; D019936:Probiotics / Q000737:chemistry / Q000627:therapeutic use*; D060888:Real-Time Polymerase Chain Reaction; D041902:Rhodobacteraceae / Q000737:chemistry; D014733:Vibrio / Q000235:genetics*; D014735:Vibrio Infections / Q000517:prevention & control / Q000662:veterinary*
references.citation: Environ Microbiol. 2006 Jul;8(7):1137-44
references.pmid: 16817922
references.citation: Appl Environ Microbiol. 2005 Nov;71(11):7263-70
references.pmid: 16269767
references.citation: Antimicrob Agents Chemother. 2011 Apr;55(4):1332-7
references.pmid: 21263047
references.citation: Appl Environ Microbiol. 2010 Apr;76(7):2366-70
references.pmid: 20118354
references.citation: Microbiol Mol Biol Rev. 2000 Dec;64(4):655-71
references.pmid: 11104813
references.citation: Indian J Exp Biol. 2001 Sep;39(9):939-42
references.pmid: 11831382
references.citation: FEMS Microbiol Rev. 2006 May;30(3):404-27
references.pmid: 16594964
references.citation: J Appl Microbiol. 2005;99(2):243-50
references.pmid: 16033454
references.citation: Syst Appl Microbiol. 2004 May;27(3):360-71
references.pmid: 15214642
references.citation: Appl Environ Microbiol. 1993 Mar;59(3):695-700
references.pmid: 7683183
references.citation: Appl Environ Microbiol. 2008 May;74(9):2595-603
references.pmid: 18326670
references.citation: Appl Environ Microbiol. 2004 Apr;70(4):2560-5
references.pmid: 15066861
references.citation: Appl Environ Microbiol. 2005 Oct;71(10):5665-77
references.pmid: 16204474
references.citation: Appl Environ Microbiol. 1995 Apr;61(4):1540-5
references.pmid: 16535003
references.citation: Dis Aquat Organ. 2008 Sep 24;81(3):203-17
references.pmid: 18998585
references.citation: Mar Biotechnol (NY). 2010 Aug;12(4):439-51
references.pmid: 19823914
references.citation: Appl Environ Microbiol. 2006 Apr;72(4):3011-5
references.pmid: 16598009
references.citation: Environ Microbiol. 2008 Jun;10(6):1571-81
references.pmid: 18331337
references.citation: Appl Environ Microbiol. 2008 Dec;74(23):7356-64
references.pmid: 18952864
references.citation: J Appl Microbiol. 2009 Dec 1;107(6):1935-46
references.pmid: 19508294
references.citation: J Appl Microbiol. 2009 Apr;106(4):1292-303
references.pmid: 19187159
references.citation: Annu Rev Microbiol. 2006;60:255-80
references.pmid: 16719716
references.citation: Appl Environ Microbiol. 2006 Aug;72(8):5547-55
references.pmid: 16885308
references.citation: Appl Environ Microbiol. 2004 Dec;70(12):7288-94
references.pmid: 15574928
references.citation: Environ Microbiol. 2002 May;4(5):287-95
references.pmid: 12030854