title: Identification of an elongation factor 1Bγ protein with glutathione transferase activity in both yeast and mycelial morphologies from human pathogenic Blastoschizomyces capitatus.
abstract: Blastoschizomyces capitatus is an uncommon, opportunistic pathogenic fungus, which causes invasive and disseminated infections. This microorganism is normally present in both environmental and normal human flora. Within a host, B. capitatus is able to grow in both unicellular yeast and multicellular filamentous growth forms. In this study, we obtained in vitro morphological conversion of B. capitatus from yeast-to-mycelial phase to investigate the presence and expression of glutathione transferase (GST) enzymes in both cell forms. A protein with GST activity using the model substrate 1-chloro-2,4-dinitrobenzene was detected in both morphologies and identified by tandem mass spectrometry as a eukaryotic elongation factor 1Bγ (eEF1Bγ) protein, a member of the GST superfamily. No significant difference in GST-specific activity and kinetic constants were observed between mycelial and yeast forms, indicating that eEF1Bγ protein did not show differential expression between the two phases.
authors.affiliation: Department of Experimental and Clinical Sciences, University "G. d'Annunzio", Via dei Vestini, 66013, Chieti, Italy, allocati@unich.it.
authors.forename: Nerino
authors.initials: N
authors.lastname: Allocati
authors.forename: Michele
authors.initials: M
authors.lastname: Masulli
authors.forename: Piero
authors.initials: P
authors.lastname: Del Boccio
authors.forename: Damiana
authors.initials: D
authors.lastname: Pieragostino
authors.forename: Domenico
authors.initials: D
authors.lastname: D'Antonio
authors.forename: David
authors.initials: D
authors.lastname: Sheehan
authors.forename: Carmine
authors.initials: C
authors.lastname: Di Ilio
mesh_terms: D004137:Dinitrochlorobenzene / Q000378:metabolism; D055157:Dipodascus / Q000166:cytology / Q000201:enzymology*; D005982:Glutathione Transferase / Q000378:metabolism*; D025282:Mycelium / Q000166:cytology / Q000201:enzymology; D020648:Peptide Elongation Factor 1 / Q000378:metabolism*; D053719:Tandem Mass Spectrometry; D015003:Yeasts / Q000166:cytology / Q000201:enzymology
chemical_list: D004137:Dinitrochlorobenzene; D020648:Peptide Elongation Factor 1; D005982:Glutathione Transferase
references.citation: Annu Rev Pharmacol Toxicol. 2005;45:51-88
references.pmid: 15822171
references.citation: Aquat Toxicol. 2008 Oct 20;90(1):48-57
references.pmid: 18804293
references.citation: FEMS Microbiol Lett. 2005 Jan 1;242(1):1-12
references.pmid: 15621414
references.citation: Biochem J. 2005 Jan 1;385(Pt 1):37-43
references.pmid: 15320869
references.citation: Methods Enzymol. 1981;77:398-405
references.pmid: 7329316
references.citation: J Clin Microbiol. 1999 Sep;37(9):2927-30
references.pmid: 10449477
references.citation: Microb Cell Fact. 2006 Mar 17;5:10
references.pmid: 16545129
references.citation: Biochem Biophys Res Commun. 2006 Mar 24;341(4):1096-104
references.pmid: 16455047
references.citation: J Clin Microbiol. 1996 Mar;34(3):753-5
references.pmid: 8904454
references.citation: J Clin Microbiol. 1994 Jan;32(1):224-7
references.pmid: 8126186
references.citation: Curr Opin Microbiol. 2007 Aug;10(4):314-9
references.pmid: 17719267
references.citation: Biochem Biophys Res Commun. 2005 Dec 2;337(4):1125-32
references.pmid: 16229819
references.citation: Mycoses. 2011 Nov;54(6):542-3
references.pmid: 20492528
references.citation: Cell Mol Life Sci. 2009 Dec;66(23):3711-25
references.pmid: 19662500
references.citation: Anal Biochem. 1976 May 7;72:248-54
references.pmid: 942051
references.citation: J Am Chem Soc. 2011 Aug 17;133(32):12322-5
references.pmid: 21749092
references.citation: J Hosp Infect. 2011 Aug;78(4):274-8
references.pmid: 21658800
references.citation: J Biol Chem. 2003 Nov 21;278(47):47190-8
references.pmid: 12972429
references.citation: RNA Biol. 2004 Jul;1(2):89-94
references.pmid: 17179749
references.citation: Biochem J. 1998 Aug 1;333 ( Pt 3):839-45
references.pmid: 9677348
references.citation: FEBS J. 2009 Jan;276(1):58-75
references.pmid: 19016852
references.citation: J Clin Microbiol. 2005 Apr;43(4):1818-28
references.pmid: 15815003
references.citation: Biochem J. 2001 Nov 15;360(Pt 1):1-16
references.pmid: 11695986
references.citation: Biochem J. 2007 Apr 15;403(2):267-74
references.pmid: 17223798
references.citation: FEMS Microbiol Lett. 2008 Jul;284(2):127-34
references.pmid: 18479435
references.citation: Biochim Biophys Acta. 2006 Jan-Feb;1759(1-2):13-31
references.pmid: 16624425
references.citation: Can J Ophthalmol. 2006 Dec;41(6):772-4
references.pmid: 17224964
references.citation: Gene. 1997 Oct 1;198(1-2):259-67
references.pmid: 9370290
references.citation: J Biol Chem. 2010 Dec 3;285(49):37995-8004
references.pmid: 20926387
references.citation: Chem Biol. 2011 Apr 22;18(4):542-52
references.pmid: 21513890