title: Roles of M2 and M3 muscarinic receptors in the generation of rhythmic motor activity in mouse small intestine.
abstract: BACKGROUND
  The roles of M2 and M3 muscarinic receptor subtypes in the regulation of gut motor activity were investigated.
  
  
  METHODS
  We simultaneously recorded changes in the intraluminal pressure (IP) and longitudinal tension (LT) in small intestinal segments from M2 or M3 receptor knockout (KO) and wild-type (WT) mice.
  
  
  RESULTS
  In the WT preparations, luminal distension induced a continuous rhythmic contractile activity that was characterized by synchronous rises in IP and LT, occurring periodically at a constant interval. Tetrodotoxin completely abolished the response, whereas atropine either abolished or attenuated it. In the majority of the M2 KO preparations, however, no rhythmic activity was observed in response to the luminal distention, even though networks of enteric neurons and interstitial cells of Cajal (ICC) seemed to be intact. Where rhythmic activity did occur in M2 KO preparations, it was atropine resistant. In the M3 KO preparations, the IP and LT were synchronously changed by the luminal distention, but the changes occurred at irregular intervals. The W/W(v) mutant preparations, which lack ICC in the myenteric plexus (ICC-MY), showed results similar to those of the M3 KO preparations. In some of the M2 /M3 double-KO preparations, rhythmic activity was not observed, but in the others, an atropine-resistant rhythmicity appeared.
  
  
  CONCLUSIONS
  These results suggest that M2 and M3 muscarinic receptors differentially regulate the intestinal motor activity: M2 receptors play an essential role in the generation of rhythmic motor activity, and M3 receptors have a modulatory role in controlling the periodicity of the rhythmic activity together with the ICC-MY.
authors.affiliation: Department of Animal Medical Sciences, Faculty of Life Sciences, Kyoto Sangyo University, Kyoto, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Tanahashi
authors.forename: N
authors.initials: N
authors.lastname: Waki
authors.forename: T
authors.initials: T
authors.lastname: Unno
authors.forename: H
authors.initials: H
authors.lastname: Matsuyama
authors.forename: S
authors.initials: S
authors.lastname: Iino
authors.forename: T
authors.initials: T
authors.lastname: Kitazawa
authors.forename: M
authors.initials: M
authors.lastname: Yamada
authors.forename: S
authors.initials: S
authors.lastname: Komori
mesh_terms: D000818:Animals; D005260:Female; D007150:Immunohistochemistry; D056885:Interstitial Cells of Cajal / Q000502:physiology; D007421:Intestine, Small / Q000502:physiology; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D018727:Muscarinic Antagonists / Q000494:pharmacology; D009119:Muscle Contraction / Q000502:physiology; D009130:Muscle, Smooth / Q000502:physiology; D009197:Myenteric Plexus / Q000502:physiology; D016065:Myoelectric Complex, Migrating / Q000502:physiology*; D043585:Receptor, Muscarinic M2 / Q000502:physiology*; D043587:Receptor, Muscarinic M3 / Q000502:physiology*
chemical_list: D018727:Muscarinic Antagonists; D043585:Receptor, Muscarinic M2; D043587:Receptor, Muscarinic M3
keywords: M2 and M3 muscarinic receptors; interstitial cells of Cajal; knockout mice; rhythmic motor activity; small intestine