title: Cooperativity and rapid evolution of cobound transcription factors in closely related mammals.
abstract: To mechanistically characterize the microevolutionary processes active in altering transcription factor (TF) binding among closely related mammals, we compared the genome-wide binding of three tissue-specific TFs that control liver gene expression in six rodents. Despite an overall fast turnover of TF binding locations between species, we identified thousands of TF regions of highly constrained TF binding intensity. Although individual mutations in bound sequence motifs can influence TF binding, most binding differences occur in the absence of nearby sequence variations. Instead, combinatorial binding was found to be significant for genetic and evolutionary stability; cobound TFs tend to disappear in concert and were sensitive to genetic knockout of partner TFs. The large, qualitative differences in genomic regions bound between closely related mammals, when contrasted with the smaller, quantitative TF binding differences among Drosophila species, illustrate how genome structure and population genetics together shape regulatory evolution.
authors.affiliation: Cancer Research UK Cambridge Institute, Li Ka Shing Centre, University of Cambridge, Cambridge CB2 0RE, UK.
authors.forename: Klara
authors.initials: K
authors.lastname: Stefflova
authors.forename: David
authors.initials: D
authors.lastname: Thybert
authors.forename: Michael D
authors.initials: MD
authors.lastname: Wilson
authors.forename: Ian
authors.initials: I
authors.lastname: Streeter
authors.forename: Jelena
authors.initials: J
authors.lastname: Aleksic
authors.forename: Panagiota
authors.initials: P
authors.lastname: Karagianni
authors.forename: Alvis
authors.initials: A
authors.lastname: Brazma
authors.forename: David J
authors.initials: DJ
authors.lastname: Adams
authors.forename: Iannis
authors.initials: I
authors.lastname: Talianidis
authors.forename: John C
authors.initials: JC
authors.lastname: Marioni
authors.forename: Paul
authors.initials: P
authors.lastname: Flicek
authors.forename: Duncan T
authors.initials: DT
authors.lastname: Odom
mesh_terms: D000818:Animals; D004330:Drosophila / Q000235:genetics; D019143:Evolution, Molecular; D008099:Liver / Q000378:metabolism; D051379:Mice / Q000145:classification* / Q000235:genetics* / Q000378:metabolism; D008815:Mice, Inbred Strains; D018345:Mice, Knockout; D051381:Rats / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism
chemical_list: D014157:Transcription Factors
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