title: Tissue laser biostimulation promotes post-extraction neoangiogenesis in HIV-infected patients.
abstract: The aim of the study was to assess the rate of neoangiogenesis in extraction wound healing following exposure to biostimulating laser therapy and to analyze the correlation between parameters of neoangiogenesis as reflected by the number and surface area of newly formed blood vessels and clinical parameters such as gender, position of a tooth in the oral cavity, and CD4 lymphocyte count. Twenty-seven patients with confirmed HIV infection were enrolled in the study (6 women, 21 men). Eighty-nine teeth were extracted; 45 sockets were exposed to 6 J laser radiation (laser parameters were set as follows: wavelength, 820 nm; output, 200 mW; dose, 6 J/cm(2); spot size, 38 mm(2); continuous radiation) for five consecutive days following tooth extraction, and the remaining extraction wounds were left to heal spontaneously without laser irradiation. Antigen CD34 was assessed by immunohistochemistry as a marker of angiogenesis, and its expression was examined by computer-assisted histomorphometric image analysis. As a result, we report that biostimulating laser therapy in HIV-infected patients of varying degrees of immunodeficiency greatly accelerated post-extraction neoangiogenesis, regardless of the patient's gender, tooth position, number of roots, or number of CD4 lymphocytes in the blood. Application of low-level laser therapy for the treatment of tooth extraction wounds in HIV(+) patients greatly enhanced the formation of new blood vessels, which in turn promoted wound healing.
authors.affiliation: Department of Pathomorphology and Oncological Cytology, Wroclaw Medical University, Borowska 213, 50-556, Wroclaw, Poland, ahalon2@gmail.com.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Halon
authors.forename: Piotr
authors.initials: P
authors.lastname: Donizy
authors.forename: Mateusz
authors.initials: M
authors.lastname: Dziegala
authors.forename: Rafal
authors.initials: R
authors.lastname: Dobrakowski
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Simon
mesh_terms: D000328:Adult; D018952:Antigens, CD34 / Q000378:metabolism; D015496:CD4-Positive T-Lymphocytes / Q000528:radiation effects; D005260:Female; D015658:HIV Infections / Q000601:surgery*; D006801:Humans; D007834:Lasers; D028022:Low-Level Light Therapy / Q000379:methods*; D008297:Male; D008875:Middle Aged; D018919:Neovascularization, Physiologic; D014081:Tooth Extraction; D020390:Tooth Socket / Q000601:surgery; D014945:Wound Healing / Q000502:physiology*
chemical_list: D018952:Antigens, CD34
references.citation: Proc Natl Acad Sci U S A. 1995 May 23;92(11):4838-42
references.pmid: 7539135
references.citation: J Bone Joint Surg Br. 2011 May;93(5):678-83
references.pmid: 21511935
references.citation: MMWR Recomm Rep. 1992 Dec 18;41(RR-17):1-19
references.pmid: 1361652
references.citation: Lasers Med Sci. 2013 Jan;28(1):325-33
references.pmid: 22814894
references.citation: Proc Natl Acad Sci U S A. 2012 Sep 4;109(36):14580-5
references.pmid: 22904195
references.citation: Arch Otolaryngol Head Neck Surg. 1996 Dec;122(12):1360-3
references.pmid: 8956750
references.citation: J Biol Regul Homeost Agents. 2012 Jul-Sep;26(3):527-37
references.pmid: 23034272
references.citation: Dis Colon Rectum. 1999 Sep;42(9):1140-4
references.pmid: 10496553
references.citation: Photodermatol Photoimmunol Photomed. 2009 Apr;25(2):75-80
references.pmid: 19292782
references.citation: Med Princ Pract. 2011;20(4):374-6
references.pmid: 21577000
references.citation: Med Microbiol Immunol. 2003 Aug;192(3):165-9
references.pmid: 12684760
references.citation: AIDS Res Hum Retroviruses. 2012 Apr;28(4):346-51
references.pmid: 21728927
references.citation: J Dent Res. 1997 Oct;76(10):1644-52
references.pmid: 9326896
references.citation: J Oral Maxillofac Surg. 2012 Aug;70(8):1885-90
references.pmid: 22079069
references.citation: HIV Med. 2004 Jan;5(1):18-25
references.pmid: 14731165
references.citation: Dermatol Surg. 2005 Mar;31(3):334-40
references.pmid: 15841638
references.citation: Dig Surg. 1999;16(1):60-7
references.pmid: 9949269
references.citation: Gen Dent. 2003 Mar-Apr;51(2):153-6
references.pmid: 15055688
references.citation: Photomed Laser Surg. 2006 Oct;24(5):588-94
references.pmid: 17069488
references.citation: J Clin Periodontol. 2008 Mar;35(3):250-4
references.pmid: 18269665
references.citation: J Bone Joint Surg Br. 2002 Aug;84(6):802-6
references.pmid: 12211668