title: Advantages of Single-Molecule Real-Time Sequencing in High-GC Content Genomes.
abstract: Next-generation sequencing has become the most widely used sequencing technology in genomics research, but it has inherent drawbacks when dealing with high-GC content genomes. Recently, single-molecule real-time sequencing technology (SMRT) was introduced as a third-generation sequencing strategy to compensate for this drawback. Here, we report that the unbiased and longer read length of SMRT sequencing markedly improved genome assembly with high GC content via gap filling and repeat resolution.
authors.affiliation: Korea Polar Research Institute, Yeonsu-gu, Incheon, Korea.
authors.forename: Seung Chul
authors.initials: SC
authors.lastname: Shin
authors.forename: Do Hwan
authors.initials: DH
authors.lastname: Ahn
authors.forename: Su Jin
authors.initials: SJ
authors.lastname: Kim
authors.forename: Hyoungseok
authors.initials: H
authors.lastname: Lee
authors.forename: Tae-Jin
authors.initials: TJ
authors.lastname: Oh
authors.forename: Jong Eun
authors.initials: JE
authors.lastname: Lee
authors.forename: Hyun
authors.initials: H
authors.lastname: Park
mesh_terms: D001482:Base Composition / Q000235:genetics*; D016680:Genome, Bacterial; D023281:Genomics; D059014:High-Throughput Nucleotide Sequencing; D008011:Lichens / Q000235:genetics / Q000382:microbiology*; D013302:Streptomyces / Q000235:genetics* / Q000302:isolation & purification
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