title: Purification, crystallization and preliminary crystallographic analysis of soybean mature glycinin A1bB2.
abstract: Glycinin is one of the most abundant storage-protein molecules in soybean seeds and is composed of five subunits (A1aB1b, A1bB2, A2B1a, A3B4 and A5A4B3). A1bB2 was purified from a mutant soybean cultivar containing glycinin composed of only A5A4B3 and A1bB2. At 281 K the protein formed hexagonal, rectangular and rod-shaped crystals in the first [0.1 M imidazole pH 8.0, 0.2 M MgCl₂, 35%(v/v) MPD], second [0.1 M sodium citrate pH 5.6, 0.2 M ammonium acetate, 30%(v/v) MPD] and third (0.1 M phosphate-citrate pH 4.2, 2.0 M ammonium sulfate) crystallization conditions, respectively. X-ray diffraction data were collected to resolutions of 1.85, 1.85 and 2.5 Å from crystals of the three different shapes. The crystals belonged to space groups P6₃22, P2₁ and P1, with unit-cell parameters a = b = 143.60, c = 84.54 Å, a = 114.54, b = 105.82, c = 116.67 Å, β = 94.99° and a = 94.45, b = 94.96, c = 100.66 Å, α = 107.02, β = 108.44, γ = 110.71°, respectively. One, six and six subunits of A1bB2 were estimated to be present in the respective asymmetric units. The three-dimensional structure of the A1bB2 hexamer is currently being determined.
authors.affiliation: Laboratory of Food Quality Design and Development, Graduate School of Agriculture, Kyoto University, Gokasho, Uji, Kyoto 611-0011, Japan.
authors.forename: Krisna
authors.initials: K
authors.lastname: Prak
authors.forename: Bunzo
authors.initials: B
authors.lastname: Mikami
authors.forename: Takafumi
authors.initials: T
authors.lastname: Itoh
authors.forename: Takako
authors.initials: T
authors.lastname: Fukuda
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Maruyama
authors.forename: Shigeru
authors.initials: S
authors.lastname: Utsumi
mesh_terms: D003460:Crystallization; D018360:Crystallography, X-Ray; D005916:Globulins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification*; D009154:Mutation / Q000235:genetics; D030262:Soybean Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification*; D013025:Glycine max
chemical_list: D005916:Globulins; D030262:Soybean Proteins; C011474:glycinin
keywords: A1bB2; glycinin; homohexamer structure; soybean
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