title: Genetic and phenotypic characterization of a Japanese wild-derived DOB/Oda rat strain.
abstract: Wild-derived rat strains can provide novel genome resources that are not available in standard laboratory strains. Genetic backgrounds of wild-derived strains can facilitate effective genetic linkage analyses and often modulate the expression of mutant phenotypes. Here we describe the development and characterization of a new inbred rat strain, DOB/Oda, from wild rats (Rattus norvegicus) captured in Shitara, Aichi, Japan. Phenotype analysis of 109 parameters revealed that the DOB/Oda rats had small body weight, preference for darkness, and high locomotor activity compared with the rat strains in the National BioResource Project for the Rat (NBRP-Rat) database. Genome analysis with 357 SSLP markers identified DOB/Oda-specific alleles in 70 markers. The percentage of SSLP markers that showed polymorphism between the DOB/Oda strain and any of 132 laboratory strains from NBRP-Rat varied from 89 to 95 %. The polymorphic rate (average of the values of the percentage) for the DOB/Oda strain was 91.6 %, much higher than the rates for available wild-derived strains such as the Brown Norway rat. A phylogenic tree constructed with DOB/Oda and all the strains in NBRP-Rat showed that the DOB/Oda strain localized within the wild rat groups, apparently separate from the laboratory strains. Together, these findings indicated that the DOB/Oda rat has a unique genome that is not available in the laboratory strains. Therefore, the new DOB/Oda strain will provide an important genome resource that will be useful for designing genetic experiments and for the discovery of genes that modulate mutant phenotypes.
authors.affiliation: Institute of Laboratory Animals, Graduate School of Medicine, Kyoto University, Yoshidakonoe-cho, Sakyo-ku, Kyoto 606-8501, Japan. tkuramot@anim.med.kyoto-u.ac.jp
authors.forename: Takashi
authors.initials: T
authors.lastname: Kuramoto
authors.forename: Satoko
authors.initials: S
authors.lastname: Inoue
authors.forename: Yuki
authors.initials: Y
authors.lastname: Neoda
authors.forename: Ken-ichi
authors.initials: K
authors.lastname: Yamasaki
authors.forename: Ryoko
authors.initials: R
authors.lastname: Hashimoto
authors.forename: Tomoji
authors.initials: T
authors.lastname: Mashimo
authors.forename: Sen-ichi
authors.initials: S
authors.lastname: Oda
authors.forename: Tadao
authors.initials: T
authors.lastname: Serikawa
mesh_terms: D000818:Animals; D001835:Body Weight / Q000235:genetics; D001947:Breeding; D005260:Female; D016678:Genome / Q000235:genetics; D005838:Genotype; D007564:Japan; D008297:Male; D010641:Phenotype; D011110:Polymorphism, Genetic; D051381:Rats; D011919:Rats, Inbred Strains / Q000235:genetics*
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