title: Statistical optimization and anticancer activity of a red pigment isolated from Streptomyces sp. PM4.
abstract: OBJECTIVE
  To enhance the pigment production by Streptomyces sp. PM4 for evaluating its anticancer activity.
  
  
  METHODS
  Response surface methodology was employed to enhance the production of red pigment from Streptomyces sp. PM4. Optimized pigment was purified and evaluated for the anticancer activity against HT1080, Hep2, HeLa and MCF7 cell lines by MTT assay.
  
  
  RESULTS
  Based on the response surface methodology, it could be concluded that maltose (4.06 g), peptone (7.34 g), yeast extract (4.34 g) and tyrosine (2.89 g) were required for the maximum production of pigment (1.68 g/L) by the Streptomyces sp. PM4. Optimization of the medium with the above tested features increased the pigment yield by 4.6 fold. Pigment showed the potential anticancer activity against HT1080, HEp-2, HeLa and MCF-7 cell lines with the IC50 value of 18.5, 15.3, 9.6 and 8.5 respectively.
  
  
  CONCLUSIONS
  The study revealed that the maximum amount of pigment could be produced to treat cancer.
authors.affiliation: CAS in Marine Biology, Faculty of Marine Sciences, Annamalai University, Parangipettai 608502, Tamil Nadu, India. vkactinobacteria@gmail.com
authors.forename: Valliappan
authors.initials: V
authors.lastname: Karuppiah
authors.forename: Chandramohan
authors.initials: C
authors.lastname: Aarthi
authors.forename: Kannan
authors.initials: K
authors.lastname: Sivakumar
authors.forename: Lakshmanan
authors.initials: L
authors.lastname: Kannan
mesh_terms: D000970:Antineoplastic Agents / Q000302:isolation & purification / Q000378:metabolism*; D045744:Cell Line, Tumor; D003470:Culture Media / Q000378:metabolism; D006801:Humans; D007218:Industrial Microbiology / Q000379:methods*; D008954:Models, Biological; D015233:Models, Statistical; D010860:Pigments, Biological / Q000302:isolation & purification / Q000378:metabolism*; D013302:Streptomyces / Q000378:metabolism*
chemical_list: D000970:Antineoplastic Agents; D003470:Culture Media; D010860:Pigments, Biological
keywords: Anticancer activity; Pigment; Response surface methodology; Streptomyces
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