title: Incorporation of chitosan nanospheres into thin mineralized collagen coatings for improving the antibacterial effect.
abstract: It is desired that the coatings on metallic implants have both excellent biological responses and good loading-release capacities of biological factors or drugs. So far, the challenge still remains, because the morphology and composition of the bioactive coatings are usually not favorable for accommodating drug molecules. In this study, we adopted an approach of incorporating chitosan nanospheres into a thin mineralized collagen coating; this approach is based on the good loading-release behavior of the nanospheres and the good cytocompatibility of the thin coating. The incorporation of chitosan nanospheres into the mineralized collagen coatings was realized by electrolytic co-deposition. The morphologies and microstructures of the resulting coatings were characterized by SEM, and the phase and chemical compositions of the coatings were measured by XRD and FTIR. The loading-release capacity for vancomycin hydrochloride (VH) was determined by ultraviolet spectrophotometry. MTS assay was used to evaluate cytocompatibility, and in vitro bacterial adhesion was tested for assessing the antibacterial effects of the VH-loaded coatings. The chitosan nanospheres adhered tightly to collagen fibrils. The incorporated coatings facilitated the sustained release of VH, and had a clear antibacterial effect. The incorporation of chitosan nanospheres into mineralized collagen coatings demonstrates an effective way to improve the drug loading-release capacity for the thin coatings. This formulation had a highly effective biological response.
authors.affiliation: Department of Materials Science and Engineering, State Key Laboratory of Silicon Materials, Zhejiang University, Hangzhou 310027, China.
authors.forename: Ziqiang
authors.initials: Z
authors.lastname: Kong
authors.affiliation: The First Affiliated Hospital of Medical College, Zhejiang University, Hangzhou 310003, China.
authors.forename: Mengfei
authors.initials: M
authors.lastname: Yu
authors.affiliation: Department of Materials Science and Engineering, State Key Laboratory of Silicon Materials, Zhejiang University, Hangzhou 310027, China. Electronic address: chengkui@zju.edu.cn.
authors.forename: Kui
authors.initials: K
authors.lastname: Cheng
authors.affiliation: Department of Materials Science and Engineering, State Key Laboratory of Silicon Materials, Zhejiang University, Hangzhou 310027, China; The Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai 200050, China. Electronic address: wengwj@zju.edu.cn.
authors.forename: Wenjian
authors.initials: W
authors.lastname: Weng
authors.affiliation: The First Affiliated Hospital of Medical College, Zhejiang University, Hangzhou 310003, China. Electronic address: wangmysm@yahoo.com.cn.
authors.forename: Huiming
authors.initials: H
authors.lastname: Wang
authors.affiliation: The First Affiliated Hospital of Medical College, Zhejiang University, Hangzhou 310003, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Lin
authors.affiliation: Department of Materials Science and Engineering, State Key Laboratory of Silicon Materials, Zhejiang University, Hangzhou 310027, China.
authors.forename: Piyi
authors.initials: P
authors.lastname: Du
authors.affiliation: Department of Materials Science and Engineering, State Key Laboratory of Silicon Materials, Zhejiang University, Hangzhou 310027, China.
authors.forename: Gaorong
authors.initials: G
authors.lastname: Han
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D016923:Cell Death / Q000187:drug effects; D002460:Cell Line; D048271:Chitosan / Q000737:chemistry*; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D003094:Collagen / Q000737:chemistry*; D007700:Kinetics; D051379:Mice; D008826:Microbial Sensitivity Tests; D008903:Minerals / Q000737:chemistry*; D054118:Nanospheres / Q000737:chemistry* / Q000648:ultrastructure; D017550:Spectroscopy, Fourier Transform Infrared; D014640:Vancomycin / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D020099:Coated Materials, Biocompatible; D008903:Minerals; D014640:Vancomycin; D003094:Collagen; D048271:Chitosan
keywords: Chitosan nanosphere; Drug-release; Mineralized collagen; Thin coating; Titanium implant