title: FoxO3a and nilotinib-induced erythroid differentiation of CML-BC cells.
abstract: We explored the potential involvement of FoxO3a activation in erythroid and granulocytic differentiation for Ph(+) cells of chronic myeloid leukemia blast crisis (CML BC). We demonstrate that FoxO3a activation in CML blast crisis (BC) cells by overexpressing FoxO3a leads to the maturation of CML BC cells. Hemoglobin production significantly increased upon FoxO3a activation in CML BC cells. FoxO3a activation upregulated erythroid surface protein (glycophorin A, GPA), but did not significantly modulate granulocytic markers (CD11b). Additionally, FoxO3a activation reduced the mRNA and protein expression of Tal1. Similar results were observed in cells that were given nilotinib. Our results indicate that FoxO3a activation may promote erythroid differentiation of BC cells via down-regulating Tal1 expression.
authors.affiliation: Department of Hematology, Southeast Hospital of Xiamen University (175 Hospital of Chinese PLA), Zhangzhou, Fujian Province, China. wangwei220012@163.com
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Nan-Nan
authors.initials: NN
authors.lastname: Li
authors.forename: Yan
authors.initials: Y
authors.lastname: Du
authors.forename: Fei-Fei
authors.initials: FF
authors.lastname: Lv
authors.forename: Guo-Qiang
authors.initials: GQ
authors.lastname: Lin
mesh_terms: D051792:Basic Helix-Loop-Helix Proteins / Q000235:genetics / Q000378:metabolism; D001752:Blast Crisis / Q000235:genetics* / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D004912:Erythrocytes / Q000166:cytology* / Q000187:drug effects*; D000071316:Forkhead Box Protein O3; D051858:Forkhead Transcription Factors / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000235:genetics* / Q000378:metabolism / Q000473:pathology*; D010766:Phosphorylation; D011518:Proto-Oncogene Proteins / Q000235:genetics / Q000378:metabolism; D000073942:T-Cell Acute Lymphocytic Leukemia Protein 1; D014162:Transfection
chemical_list: D051792:Basic Helix-Loop-Helix Proteins; D000071316:Forkhead Box Protein O3; D051858:Forkhead Transcription Factors; D011518:Proto-Oncogene Proteins; D000073942:T-Cell Acute Lymphocytic Leukemia Protein 1; C499517:FOXO3 protein, human; C067143:TAL1 protein, human
keywords: Blast crisis; Chronic myeloid leukemia; Erythroid differentiation; FoxO3a; Granulocytic differentiation