title: Bioactive baculovirus nanohybrids for stent based rapid vascular re-endothelialization.
abstract: Present study, for the first time, reports the development of a nanohybridized baculovirus based stent that can locally promote vascular re-endothelialization by efficient delivery of pro-angiogenic vascular endothelial growth factor (Vegf) genes. In vitro data demonstrated rapid expression of functionally active Vegf by the bioactive stent-transduced vascular cells. In vivo site-specific transgene expression was observed at the stented regions of balloon-denuded canine femoral artery, which eventually lead to significant endothelial recovery at the injured sites. A significant reduction in neointima formation (2.23 ± 0.56 mm(2) vs 2.78 ± 0.49 mm(2) and 3.11 ± 0.23 mm(2), p < 0.05; n = 8) and percent stenosis was observed in treated stent group compared to negative control and bare metal stent groups. These findings collectively implicate the potential of this newly developed baculovirus based biotherapeutic stent to ameliorate damaged vascular biology and attenuate re-narrowing of stented artery by inhibiting neointima formation.
authors.affiliation: Biomedical Technology and Cell Therapy Research Laboratory, Department of Biomedical Engineering, Faculty of Medicine, McGill University, 3775 University Street, Montreal, Quebec, Canada.
authors.forename: Arghya
authors.initials: A
authors.lastname: Paul
authors.forename: Cynthia B
authors.initials: CB
authors.lastname: Elias
authors.forename: Dominique
authors.initials: D
authors.lastname: Shum-Tim
authors.forename: Satya
authors.initials: S
authors.lastname: Prakash
mesh_terms: D000818:Animals; D016367:Baculoviridae / Q000235:genetics*; D001807:Blood Vessel Prosthesis; D004285:Dogs; D019544:Equipment Failure Analysis; D005263:Femoral Artery / Q000254:growth & development* / Q000601:surgery; D036103:Nanotechnology / Q000295:instrumentation*; D011474:Prosthesis Design; D012038:Regeneration / Q000502:physiology; D015607:Stents; D014161:Transduction, Genetic / Q000379:methods*; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics* / Q000627:therapeutic use*
chemical_list: D042461:Vascular Endothelial Growth Factor A
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