title: [Roles of bFGF in endothelial cell tube formation induced by xuefu zhuyu decoction under non-anoxia condition].
abstract: OBJECTIVE
  To explore the roles of basic fibroblast growth factor (bFGF) on tube formation induced by xuefu zhuyu decoction (XZD) under non-anoxia condition.
  
  
  METHODS
  Using serum pharmacology technique, endothelial cell line ECV304 cells were incubated in routine 95% O2. ECV304 cells were intervened by 1.25%, 2.50%, and 5.00% XZD containing serums and the vehicle serum for 48 h. The effects of XZD on tube formation, bFGF contents and its transcription levels were assessed by in vitro tube formation assay, enzyme-linked immunosorbent assay (ELISA), and reverse transcriptase polymerase chain reaction (RT-PCR), respectively.
  
  
  RESULTS
  Three concentrations of XZD containing serums could not only obviously promote the tube formation bFGF level, but also up-regulate bFGF contents in the supernate and its transcription levels. The shapes of lumens were more regular in those induced by 1.25% and 2.50% XZD containing serums.
  
  
  CONCLUSIONS
  XZD induced angiogenesis via up-regulating the bFGF expression under non-anoxia condition.
authors.affiliation: Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou 350108, China.
authors.forename: Dong
authors.initials: D
authors.lastname: Gao
authors.forename: Jing-Si
authors.initials: JS
authors.lastname: Zhang
authors.forename: Ya-Qiong
authors.initials: YQ
authors.lastname: Hu
authors.forename: Fan
authors.initials: F
authors.lastname: Lin
authors.forename: Yi-Zheng
authors.initials: YZ
authors.lastname: Wang
authors.forename: Jun
authors.initials: J
authors.lastname: Song
authors.forename: Ke-ji
authors.initials: KJ
authors.lastname: Chen
mesh_terms: D000818:Animals; D002460:Cell Line; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D042783:Endothelial Cells / Q000187:drug effects / Q000378:metabolism*; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism*; D006801:Humans; D008297:Male; D018919:Neovascularization, Physiologic / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D004365:Drugs, Chinese Herbal; C513826:Xue-Fu-Zhu-Yu decoction; D016222:Fibroblast Growth Factor 2