title: Inhibition of toll-like receptor 2-mediated NF-kappaB activation in Vero cells with herpesvirus of turkeys.
abstract: In a previous study, vaccination with a live bivalent vaccine consisting of herpesvirus of turkeys (HVT) and SB-1 was found to be associated with distinct cytokine expression patterns and the modulation of cytokine responses in the spleen. This vaccine could play a role in mediating protection against infection with the RB1B strain of Marek's disease virus. In the present study, vectors for chicken Toll-like receptor 1 (chTLR1) and 2 (chTLR2) expression were constructed and transfected into Vero cells. Nuclear factor kappa light-chain enhancer of activated B cell (NF-kappaB) activation was detected after HVT infection. Compared with normal Vero cells, NF-kappaB activation was significantly inhibited by HVT in Vero cells transfected with chTLR1-1, chTLR1-2, or both. The results demonstrate the significant characteristics of HVT in activating TLR2 signaling. chTLR1 plays a key role in TLR2 subfamily-mediated NF-kappaB inhibition after HVT infection.
authors.affiliation: Guangxi University, Nanning, Guangxi, 530004, People's Republic of China.
authors.forename: Qingli
authors.initials: Q
authors.lastname: Yang
authors.forename: Hao
authors.initials: H
authors.lastname: Chen
authors.forename: Tianchao
authors.initials: T
authors.lastname: Wei
authors.forename: Ping
authors.initials: P
authors.lastname: Wei
mesh_terms: D000818:Animals; D002645:Chickens / Q000276:immunology* / Q000378:metabolism; D002522:Chlorocebus aethiops; D005786:Gene Expression Regulation; D026002:Herpesvirus 1, Meleagrid / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D016328:NF-kappa B / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction; D051194:Toll-Like Receptor 1 / Q000378:metabolism*; D051195:Toll-Like Receptor 2 / Q000378:metabolism*; D014709:Vero Cells
chemical_list: D016328:NF-kappa B; D011994:Recombinant Proteins; D051194:Toll-Like Receptor 1; D051195:Toll-Like Receptor 2