title: A homogeneous hemin/G-quadruplex DNAzyme based turn-on chemiluminescence aptasensor for interferon-gamma detection via in-situ assembly of luminol functionalized gold nanoparticles, deoxyribonucleic acid, interferon-gamma and hemin.
abstract: A homogeneous hemin/G-quadruplex DNAzyme (HGDNAzyme) based turn-on chemiluminescence aptasensor for interferon-gamma (IFN-γ) detection is developed, via dynamic in-situ assembly of luminol functionalized gold nanoparticles (lum-AuNPs), DNA, IFN-γ and hemin. The G-quadruplex oligomer of the HGDNAzyme was split into two halves, which was connected with the complementary sequence of P1 (IFN-γ-binding aptamer) to form the oligonucleotide P2. P2 hybridized with IFN-γ-binding aptamer and meanwhile assembled onto lum-AuNPs through biotin-streptavidin specific interaction. When IFN-γ was recognized by aptamer, P2 was released into the solution. The two lateral portions of P2 combined with hemin to yield the catalytic hemin/G-quadruplex DNAzyme, which amplified the luminol oxidation for a turn-on chemiluminescence signaling. Based on this strategy, the homogeneous aptasensor enables the facile detection of IFN-γ in a range of 0.5-100 nM. Moreover, the aptasensor showed high sensitivity (0.4 nM) and satisfactory specificity, pointing to great potential applications in clinical analysis.
authors.affiliation: CAS Key Laboratory of Soft Matter Chemistry, Department of Chemistry, University of Science and Technology of China, Hefei, Anhui 230026, PR China.
authors.forename: Jie
authors.initials: J
authors.lastname: Jiang
authors.forename: Yi
authors.initials: Y
authors.lastname: He
authors.forename: Xiuxia
authors.initials: X
authors.lastname: Yu
authors.forename: Jinyang
authors.initials: J
authors.lastname: Zhao
authors.forename: Hua
authors.initials: H
authors.lastname: Cui
mesh_terms: D001483:Base Sequence; D004247:DNA / Q000737:chemistry*; D021881:DNA, Catalytic / Q000737:chemistry*; D054856:G-Quadruplexes; D006046:Gold / Q000737:chemistry*; D006427:Hemin / Q000737:chemistry*; D007371:Interferon-gamma / Q000032:analysis*; D049449:Luminescence; D008165:Luminol / Q000737:chemistry*; D053768:Metal Nanoparticles
chemical_list: D021881:DNA, Catalytic; D008165:Luminol; D006427:Hemin; D006046:Gold; D007371:Interferon-gamma; D004247:DNA
keywords: Aptasensor; Chemiluminescence; DNAzyme; Homogeneous; Interferon-gamma; Luminol functionalized gold nanoparticles