title: Performance of an enzymatic extract in Botrycoccus braunii cell wall disruption.
abstract: Microalgae can produce and contain lipids, proteins and carbohydrates, which can be extracted and marketed as potential novel added-value bio-products. However, microalgae cell wall disruption is one of the most important challenges involved while processing this type of biomass. In this context, white-rot fungi, responsible for the biodegradation of lignin present in wood due to non-specific extracellular enzymes, could be applied for promoting microalgae cell wall degradation. Therefore, the aim of this study was to evaluate the use of an enzymatic extract produced by the white-rot fungi Anthracophyllum discolor as a biotechnological tool for Botryococcus braunii cell wall disruption. The fungus was inoculated in wheat grains and manganese peroxidase (MnP) activity was monitored while obtaining the enzymatic extract. Then, cell wall disruption trials with different MnP activity were evaluated by the biochemical methane potential (BMP). In relation to cell wall disruption, it was observed that the optimal value was obtained with enzymatic concentration of 1000 U/L with a BMP of 521 mL CH4/g VS. Under these conditions almost 90% of biomass biodegradability was observed, increasing in 62% compared to the microalgae without treatment. Therefore, the results indicate that enzymes secreted by A. discolor promoted the attack of the different cell wall components finally weakening it. Therefore, the application of this treatment could be a promissory biotechnological approach to decrease the energetic input required for the cell wall disruption step.
authors.affiliation: Scientific and Technological Bioresource Nucleus, Universidad de La Frontera, Casilla 54-D, Temuco, Chile; Departamento de Ingeniería Química, Universidad de La Frontera, Casilla 54-D, Temuco, Chile. Electronic address: gustavo.ciudad@ufrontera.cl.
authors.forename: Gustavo
authors.initials: G
authors.lastname: Ciudad
authors.affiliation: Scientific and Technological Bioresource Nucleus, Universidad de La Frontera, Casilla 54-D, Temuco, Chile; Departamento de Ingeniería Química, Universidad de La Frontera, Casilla 54-D, Temuco, Chile. Electronic address: olga.rubilar@ufrontera.cl.
authors.forename: Olga
authors.initials: O
authors.lastname: Rubilar
authors.affiliation: Scientific and Technological Bioresource Nucleus, Universidad de La Frontera, Casilla 54-D, Temuco, Chile. Electronic address: laura.azocar@ufrontera.cl.
authors.forename: Laura
authors.initials: L
authors.lastname: Azócar
authors.affiliation: Centro de Investigación de Polímeros Avanzados, CIPA, Beltrán Mathieu 224 piso 2, Barrio Universitario, Concepción, Chile. Electronic address: c.toro@cipachile.cl.
authors.forename: Claudio
authors.initials: C
authors.lastname: Toro
authors.affiliation: Scientific and Technological Bioresource Nucleus, Universidad de La Frontera, Casilla 54-D, Temuco, Chile. Electronic address: mara.cea@ufrontera.cl.
authors.forename: Mara
authors.initials: M
authors.lastname: Cea
authors.affiliation: Scientific and Technological Bioresource Nucleus, Universidad de La Frontera, Casilla 54-D, Temuco, Chile. Electronic address: alvarotorres@ufro.cl.
authors.forename: Álvaro
authors.initials: Á
authors.lastname: Torres
authors.affiliation: Center of Plant-Soil Interaction and Natural Resources Biotechnology, Scientific and Technological Bioresource Nucleus (BIOREN), Universidad de La Frontera, Casilla 54-D, Temuco, Chile. Electronic address: alejandra.ribera@ufrontera.cl.
authors.forename: Alejandra
authors.initials: A
authors.lastname: Ribera
authors.affiliation: Scientific and Technological Bioresource Nucleus, Universidad de La Frontera, Casilla 54-D, Temuco, Chile; Departamento de Ingeniería Química, Universidad de La Frontera, Casilla 54-D, Temuco, Chile. Electronic address: rodrigo.navia@ufro.cl.
authors.forename: Rodrigo
authors.initials: R
authors.lastname: Navia
mesh_terms: D000363:Agaricales / Q000201:enzymology* / Q000254:growth & development; D001487:Basidiomycota / Q000201:enzymology*; D001673:Biodegradation, Environmental; D018533:Biomass; D002473:Cell Wall / Q000378:metabolism*; D008031:Lignin / Q000378:metabolism; D008697:Methane / Q000032:analysis; D010544:Peroxidases / Q000302:isolation & purification / Q000378:metabolism*; D014908:Triticum / Q000382:microbiology
chemical_list: C036909:lignocellulose; D008031:Lignin; D010544:Peroxidases; C051129:manganese peroxidase; D008697:Methane
keywords: Anthracophyllum discolor; Biochemical methane potential; Cell wall disruption; Ligninolytic enzymes; Microalgae