title: Development of Next-Generation Peptide Binders Using In vitro Display Technologies and Their Potential Applications.
abstract: During the last decade, a variety of monoclonal antibodies have been developed and used as molecular targeting drugs in medical therapies. Although antibody drugs tend to have intense pharmacological activities and negligible side effects, several issues in their development and prescription remain to be resolved. Synthetic peptides with affinities and specificities for a desired target have received significant attention as alternatives to antibodies. In vitro display technologies are powerful methods for the selection of such peptides from combinatorial peptide libraries. Various types of peptide binders are being selected with such technologies for use in a wide range of fields from bioscience to medicine. This mini review article focuses on the current state of in vitro display selection of synthetic peptide binders and compares the selected peptides with natural peptides/proteins to provide a better understanding of the target affinities and inhibitory activities derived from their amino acid sequences and structural frameworks. The potential of synthetic peptide binders as alternatives to antibody drugs in therapeutic applications is also reviewed.
authors.affiliation: RIKEN , Saitama , Japan.
authors.forename: Akira
authors.initials: A
authors.lastname: Wada
keywords: antibody drug; in vitro display technology; peptide binder; peptide therapeutic; target binding
references.citation: Nat Chem Biol. 2009 Jul;5(7):502-7
references.pmid: 19483697
references.citation: Chem Biol. 2008 Jan;15(1):32-42
references.pmid: 18215771
references.citation: Proc Natl Acad Sci U S A. 2012 Jul 10;109(28):11121-6
references.pmid: 22723348
references.citation: Biochemistry. 2007 Sep 4;46(35):10102-12
references.pmid: 17685586
references.citation: Curr Opin Chem Biol. 2008 Dec;12(6):692-7
references.pmid: 18793750
references.citation: J Med Chem. 2010 Jun 10;53(11):4441-9
references.pmid: 20476787
references.citation: Nat Methods. 2007 Mar;4(3):281-8
references.pmid: 17327849
references.citation: Acc Chem Res. 2011 Apr 19;44(4):261-8
references.pmid: 21366289
references.citation: Nat Biotechnol. 2001 Aug;19(8):751-5
references.pmid: 11479568
references.citation: Chem Biol. 2000 Jan;7(1):17-25
references.pmid: 10662687
references.citation: Nat Biotechnol. 2000 Dec;18(12):1287-92
references.pmid: 11101809
references.citation: Chem Biol. 2003 Mar;10(3):233-9
references.pmid: 12670537
references.citation: Biochemistry. 1995 Nov 28;34(47):15430-5
references.pmid: 7492543
references.citation: Nature. 2000 Jun 8;405(6787):665-8
references.pmid: 10864319
references.citation: Nat Commun. 2012 Jul 17;3:951
references.pmid: 22805558
references.citation: Methods Mol Biol. 2010;607:219-25
references.pmid: 20204860
references.citation: J Am Chem Soc. 2012 Jun 27;134(25):10469-77
references.pmid: 22428867
references.citation: Proc Natl Acad Sci U S A. 2004 Mar 2;101(9):2806-10
references.pmid: 14981246
references.citation: Chem Rev. 2001 Oct;101(10):3205-18
references.pmid: 11710069
references.citation: Proc Natl Acad Sci U S A. 2007 Oct 23;104(43):16828-33
references.pmid: 17942675
references.citation: J Mol Biol. 2006 Oct 13;363(1):75-97
references.pmid: 16962610
references.citation: Chem Rev. 1997 Apr 1;97(2):391-410
references.pmid: 11848876
references.citation: Nat Methods. 2006 Nov;3(11):923-9
references.pmid: 17060916
references.citation: Biotechnol Bioeng. 2008 Dec 1;101(5):1102-7
references.pmid: 18613123
references.citation: Nat Biotechnol. 2011 Mar;29(3):245-54
references.pmid: 21390033
references.citation: Biochemistry. 1992 Oct 6;31(39):9350-4
references.pmid: 1390720
references.citation: J Mol Biol. 1999 Jan 8;285(1):197-203
references.pmid: 9878399
references.citation: Org Lett. 2012 Mar 2;14(5):1194-7
references.pmid: 22332901
references.citation: Nucleic Acids Res. 2004 Dec 02;32(21):e169
references.pmid: 15576676
references.citation: Nat Methods. 2007 Mar;4(3):269-79
references.pmid: 17327848
references.citation: Curr Opin Chem Biol. 2002 Dec;6(6):872-7
references.pmid: 12470744
references.citation: Nature. 1991 Nov 7;354(6348):82-4
references.pmid: 1944576
references.citation: Nat Biotechnol. 2005 Oct;23(10):1257-68
references.pmid: 16211069
references.citation: J Mol Biol. 2000 Mar 24;297(2):309-19
references.pmid: 10715203
references.citation: Proc Natl Acad Sci U S A. 1994 Sep 13;91(19):9022-6
references.pmid: 7522328
references.citation: Science. 1990 Jul 27;249(4967):404-6
references.pmid: 2143033
references.citation: Methods Enzymol. 2012;503:101-34
references.pmid: 22230567
references.citation: Chem Biol. 2011 Dec 23;18(12):1562-70
references.pmid: 22195558
references.citation: Biochemistry. 1995 Nov 28;34(47):15421-9
references.pmid: 7492542
references.citation: ChemMedChem. 2013 Mar;8(3):377-84
references.pmid: 23355488
references.citation: J Mol Recognit. 2010 Nov-Dec;23(6):559-68
references.pmid: 21038356
references.citation: ACS Comb Sci. 2011 Sep 12;13(5):478-85
references.pmid: 21766868
references.citation: Mol Cancer Ther. 2008 Jun;7(6):1461-71
references.pmid: 18566217
references.citation: Science. 1997 Jun 13;276(5319):1696-9
references.pmid: 9180079
references.citation: Nat Mater. 2003 Mar;2(3):196-200
references.pmid: 12612679
references.citation: Immunol Today. 2000 Aug;21(8):397-402
references.pmid: 10916143
references.citation: J Am Chem Soc. 2005 Aug 24;127(33):11727-35
references.pmid: 16104750
references.citation: Methods Mol Biol. 2012;805:45-57
references.pmid: 22094799
references.citation: Nat Protoc. 2011 Jul 21;6(8):1163-82
references.pmid: 21799486
references.citation: Sci Rep. 2012;2:691
references.pmid: 23056904
references.citation: Nat Protoc. 2007;2(12):3063-73
references.pmid: 18079705
references.citation: J Mol Biol. 2003 May 30;329(2):381-8
references.pmid: 12758084
references.citation: ACS Chem Biol. 2007 Sep 21;2(9):625-34
references.pmid: 17894440
references.citation: Cancer Res. 2005 Jun 1;65(11):4827-35
references.pmid: 15930303
references.citation: Curr Opin Immunol. 2008 Aug;20(4):460-70
references.pmid: 18656541