title: A novel anaerobic co-culture system for bio-hydrogen production from sugarcane bagasse.
abstract: A novel co-culture of Clostridium thermocellum and Thermoanaerobacterium aotearoense with pretreated sugarcane bagasse (SCB) under mild alkali conditions for bio-hydrogen production was established, exhibiting a cost-effective and synergetic advantage in bio-hydrogen production over monoculture of C. thermocellum or T. aotearoense with untreated SCB. The optimized pretreatment conditions were established to be 3% NaOH, and a liquid to solid ratio of 25:1 at 80°C for 3h. A final hydrogen production of 50.05±1.51 mmol/L was achieved with 40 g/L pretreated SCB at 55°C. The established co-culture system provides a novel consolidated bio-processing strategy for bioconversion of SCB to bio-hydrogen.
authors.affiliation: School of Bioscience and Bioengineering, South China University of Technology, Guangzhou Higher Education Mega Center, Panyu, Guangzhou 510006, People's Republic of China.
authors.forename: Jingrong
authors.initials: J
authors.lastname: Cheng
authors.forename: Mingjun
authors.initials: M
authors.lastname: Zhu
mesh_terms: D000641:Ammonia / Q000494:pharmacology; D000693:Anaerobiosis / Q000187:drug effects; D056804:Biofuels / Q000382:microbiology*; D002482:Cellulose / Q000378:metabolism*; D048013:Acetivibrio thermocellus / Q000187:drug effects / Q000378:metabolism*; D018920:Coculture Techniques / Q000379:methods*; D005285:Fermentation / Q000187:drug effects; D006859:Hydrogen / Q000378:metabolism*; D006861:Hydrogen Peroxide / Q000494:pharmacology; D031786:Saccharum / Q000737:chemistry* / Q000187:drug effects; D012972:Sodium Hydroxide / Q000494:pharmacology; D013696:Temperature; D046950:Thermoanaerobacterium / Q000187:drug effects / Q000378:metabolism*; D013997:Time Factors
chemical_list: D056804:Biofuels; D012972:Sodium Hydroxide; D000641:Ammonia; D006859:Hydrogen; D002482:Cellulose; C027433:bagasse; D006861:Hydrogen Peroxide
keywords: Bio-hydrogen; Co-culture; Mild alkali pretreatment; Sugarcane bagasse