title: Basic transcription factor 3 is involved in gastric cancer development and progression.
abstract: OBJECTIVE
  To further analyse cancer involvement of basic transcription factor 3 (BTF3) after detection of its upregulation in gastric tumor samples.
  
  
  METHODS
  BTF3 transcription rates in human gastric tumor tissue samples (n = 20) and adjacent normal tissue (n = 18) specimens as well as in the gastric cancer cell lines AGS, SGC-7901, MKN-28, MKN-45 and MGC803 were analyzed via quantitative real-time polymerase chain reaction. The effect of stable BTF3 silencing via infection with a small interfering RNA (siRNA)-BTF3 expressing lentivirus on SGC-7901 cells was measured via Western blotting analysis, proliferation assays, cell cycle and apoptosis profiling by flow cytometry as well as colony forming assays with a Cellomic Assay System.
  
  
  RESULTS
  A significant higher expression of BTF3 mRNA was detected in tumors compared to normal gastric tissues (P < 0.01), especially in section tissues from female patients compared to male patients, and all tested gastric cancer cell lines expressed high levels of BTF3. From days 1 to 5, the relative proliferation rates of stable BTF3-siRNA transfected SGC7901 cells were 82%, 70%, 57%, 49% and 44% compared to the control, while the percentage of cells arrested in the G1 phase was significantly decreased (P = 0.000) and the percentages of cells in the S (P = 0.031) and G2/M (P = 0.027) phases were significantly increased. In addition, the colony forming tendency was significantly decreased (P = 0.014) and the apoptosis rate increased from 5.73% to 8.59% (P = 0.014) after BTF3 was silenced in SGC7901 cells.
  
  
  CONCLUSIONS
  BTF3 expression is associated with enhanced cell proliferation, reduced cell cycle regulation and apoptosis and its silencing decreased colony forming and proliferation of gastric cancer cells.
authors.affiliation: Department of Anorectal Surgery, People's Hospital of Hunan Province, First Affiliated Hospital of Hunan Normal University, Changsha 410005, Hunan Province, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Liu
authors.forename: Jian-Ping
authors.initials: JP
authors.lastname: Zhou
authors.forename: Bin
authors.initials: B
authors.lastname: Li
authors.forename: Zhong-Cheng
authors.initials: ZC
authors.lastname: Huang
authors.forename: Hong-Yu
authors.initials: HY
authors.lastname: Dong
authors.forename: Guang-Yi
authors.initials: GY
authors.lastname: Li
authors.forename: Ke
authors.initials: K
authors.lastname: Zhou
authors.forename: Shao-Lin
authors.initials: SL
authors.lastname: Nie
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D017209:Apoptosis; D002453:Cell Cycle; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D008875:Middle Aged; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D034622:RNA Interference; D013274:Stomach Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D013997:Time Factors; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D014162:Transfection; D015854:Up-Regulation
chemical_list: D009687:Nuclear Proteins; D014157:Transcription Factors; C063552:transcription factor BTF3
keywords: Apoptosis; Basic transcription factor 3; Cell cycle; Gastric cancer; Proliferation; Small interfering RNA
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