title: Microwave-assisted extraction followed by RP-HPLC for the simultaneous extraction and determination of forsythiaside A, rutin, and phillyrin in the fruits of Forsythia suspensa.
abstract: An optimized microwave-assisted extraction (MAE) method and RP-HPLC method were developed for the simultaneous extraction and determination of rutin, forsythiaside A, and phillyrin in the fruits of Forsythia suspensa. The key parameters of the open-vessel MAE process were optimized. A mixed solvent of methanol and water (70:30, v/v) was most suitable for the simultaneous extraction of the three components. The sample was soaked for 10 min before extraction. The optimized conditions were: microwave power 400 W, temperature 70°C, solvent-to-material ratio 30 mL/g, and extraction time 1 min. Compared to conventional extraction methods, the proposed method can simultaneously extract the three components in high yields and was proved to be a more rapid method with a lower solvent consumption. The optimized HPLC-photodiode array detection analysis was validated to have good linearity, precision, accuracy, and sensitivity. The developed MAE followed by RP-HPLC is a fast and appropriate method for the simultaneous extraction and determination of rutin, forsythiaside A, and phillyrin in the fruits of F. suspensa.
authors.affiliation: State Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural University, Taian, China.
authors.forename: Xinsheng
authors.initials: X
authors.lastname: Fang
authors.forename: Yingzi
authors.initials: Y
authors.lastname: Wang
authors.forename: Jianhua
authors.initials: J
authors.lastname: Wang
authors.forename: Jin
authors.initials: J
authors.lastname: Zhang
authors.forename: Xin
authors.initials: X
authors.lastname: Wang
mesh_terms: D005591:Chemical Fractionation / Q000295:instrumentation / Q000379:methods*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D031657:Forsythia / Q000737:chemistry*; D005638:Fruit / Q000737:chemistry; D005960:Glucosides / Q000032:analysis / Q000302:isolation & purification*; D006027:Glycosides / Q000032:analysis / Q000302:isolation & purification*; D008872:Microwaves; D010936:Plant Extracts / Q000032:analysis / Q000302:isolation & purification*; D012431:Rutin / Q000032:analysis / Q000302:isolation & purification*
chemical_list: D005960:Glucosides; D006027:Glycosides; D010936:Plant Extracts; D012431:Rutin; C058049:forsythiaside; C075528:phillyrin
keywords: Forsythiaside A; High-performance liquid chromatography; Microwave-assisted extraction; Phillyrin; Rutin