title: [Extraction of fatty constituents from Tabanus bivittatus and their analysis by GC-MS].
abstract: OBJECTIVE
  To optimize the extracting condition of fatty constituents from Tabanus bivittatus and analyse by GC-MS.
  
  
  METHODS
  Taking fatty yield as index, the extraction technology parameters of fatty constituents of Tabanus bivittatus ere optimized by single factor test and orthogonal test.
  
  
  RESULTS
  The optimum extraction parameters were as follows: the ratio of acetone to petroleum ether 1: 2, extraction time 2 h, solid-liquid ratio 1: 50. Twenty-one fatty acids were identified by GC-MS.
  
  
  CONCLUSIONS
  The process is reasonable and with good reproducibility. The main components of fatty acids in Tabanus bivittatus are palmitoleic acid, palmitic acid, linoleic acid, oleic acid and stearic acid.
authors.affiliation: School of Chemistry and Pharmaceutical Engineering/School of Life Science and Technology, Nanyang Normal University, Nanyang 473061, China. dingdingxy2004@163.com
authors.forename: Cheng-Hua
authors.initials: CH
authors.lastname: Ding
authors.forename: Feng-Pu
authors.initials: FP
authors.lastname: Cao
authors.forename: Yan-Hua
authors.initials: YH
authors.lastname: Wang
authors.forename: Ming-Li
authors.initials: ML
authors.lastname: Yang
mesh_terms: D000096:Acetone / Q000737:chemistry; D000818:Animals; D004175:Diptera / Q000737:chemistry*; D005227:Fatty Acids / Q000032:analysis* / Q000302:isolation & purification; D008401:Gas Chromatography-Mass Spectrometry; D019787:Linoleic Acid / Q000032:analysis; D008419:Materia Medica / Q000032:analysis* / Q000302:isolation & purification; D019301:Oleic Acid / Q000032:analysis; D019308:Palmitic Acid / Q000032:analysis; D015203:Reproducibility of Results; D012997:Solvents / Q000737:chemistry; D013997:Time Factors
chemical_list: D005227:Fatty Acids; D008419:Materia Medica; D012997:Solvents; D000096:Acetone; D019301:Oleic Acid; D019308:Palmitic Acid; D019787:Linoleic Acid