title: A new effective process for production of curdlan oligosaccharides based on alkali-neutralization treatment and acid hydrolysis of curdlan particles in water suspension.
abstract: Biologically active β-1,3-oligosaccharides with rapidly growing biomedical applications are produced from hydrolysis of curdlan polysaccharide. The water-insoluble curdlan impedes its hydrolysis efficiency which is enhanced by our newly developed alkali-neutralization treatment process to increase the stability of curdlan suspension to more than 20 days, while the untreated control settled within 5 min. A putative double-layer structure model comprising of a compact core and a hydrated outer layer was proposed to describe the treated curdlan particles based on sedimentation and scanning electron microscopy observation. This model was verified by single- and two-step acid hydrolysis, indicative of the reduced susceptibility to hydrolysis when close to the compact core. Electrospray ionization-mass spectrometry, thin-layer chromatography analyses, and effective HPLC procedure led to the development of improved process to produce purified individual β-1,3-oligosaccharides with degrees of polymerization from 2 to 10 and potential for biomedical applications from curdlan hydrolyzate. Our new curdlan oligosaccharide production process offers an even better alternative to the previously published processes.
authors.affiliation: Key Laboratory of Carbohydrate Chemistry and Biotechnology of Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, Jiangsu, 214122, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.forename: Li
authors.initials: L
authors.lastname: Zhu
authors.forename: Zhi-Yong
authors.initials: ZY
authors.lastname: Zheng
authors.forename: Xiao-Bei
authors.initials: XB
authors.lastname: Zhan
authors.forename: Chi-Chung
authors.initials: CC
authors.lastname: Lin
authors.forename: Yu
authors.initials: Y
authors.lastname: Zong
authors.forename: Wei-Jiang
authors.initials: WJ
authors.lastname: Li
mesh_terms: D000143:Acids / Q000737:chemistry*; D000468:Alkalies / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D006868:Hydrolysis; D008855:Microscopy, Electron, Scanning; D009844:Oligosaccharides / Q000302:isolation & purification*; D021241:Spectrometry, Mass, Electrospray Ionization; D014867:Water; D047071:beta-Glucans / Q000737:chemistry*
chemical_list: D000143:Acids; D000468:Alkalies; D009844:Oligosaccharides; D047071:beta-Glucans; D014867:Water; C038459:curdlan