title: Chitosan tripolyphosphate (CS/TPP) nanoparticles: preparation, characterization and application for gene delivery in shrimp.
abstract: The present study examines the use of CS/TPP nanoparticles for gene delivery in different tissues of shrimp through oral route. The viral gene of WSSV was used to construct DNA vaccines using pcDNA 3.1, a eukaryotic expression vector and the constructs were named as pVP28. The CS/TPP nanoparticles were synthesized by ionic gelation process and these particles were characterized. The structure and morphology of the nanoparticles were studied by field emission scanning electron microscopy (FE-SEM) and FTIR (Fourier Transform Infrared Spectra). The cytotoxicity of CS/TPP nanoparticles was evaluated by MTT assay using fish cell line. The expression of gene was confirmed by Immuno-dot blot, ELISA and RT-PCR analyses. The results indicate that DNA can be easily delivered into shrimp by feeding with CS/TPP nanoparticles.
authors.affiliation: OIE Reference Laboratory for WTD, Aquaculture Biotechnology Division, PG & Research Department of Zoology, C. Abdul Hakeem College, Melvisharam 632509, Vellore District, Tamil Nadu, India.
authors.forename: S
authors.initials: S
authors.lastname: Vimal
authors.forename: S
authors.initials: S
authors.lastname: Abdul Majeed
authors.forename: G
authors.initials: G
authors.lastname: Taju
authors.forename: K S N
authors.initials: KS
authors.lastname: Nambi
authors.forename: N
authors.initials: N
authors.lastname: Sundar Raj
authors.forename: N
authors.initials: N
authors.lastname: Madan
authors.forename: M A
authors.initials: MA
authors.lastname: Farook
authors.forename: T
authors.initials: T
authors.lastname: Rajkumar
authors.forename: D
authors.initials: D
authors.lastname: Gopinath
authors.forename: A S
authors.initials: AS
authors.lastname: Sahul Hameed
mesh_terms: D000284:Administration, Oral; D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D048271:Chitosan / Q000008:administration & dosage* / Q000633:toxicity; D003445:Crustacea / Q000235:genetics*; D005399:Fishes; D018014:Gene Transfer Techniques; D008855:Microscopy, Electron, Scanning; D053758:Nanoparticles / Q000008:administration & dosage* / Q000633:toxicity / Q000648:ultrastructure; D011122:Polyphosphates / Q000008:administration & dosage* / Q000633:toxicity; D017550:Spectroscopy, Fourier Transform Infrared; D019444:Vaccines, DNA / Q000008:administration & dosage* / Q000235:genetics / Q000633:toxicity; D014765:Viral Vaccines / Q000008:administration & dosage* / Q000235:genetics / Q000633:toxicity; D046848:White spot syndrome virus 1 / Q000235:genetics
chemical_list: D011122:Polyphosphates; D019444:Vaccines, DNA; D014765:Viral Vaccines; D048271:Chitosan; C005692:triphosphoric acid
keywords: CS/TPP nanoparticles; Immuno-dot blot; In vivo gene expression; Real-time PCR; pVP28 DNA