title: Infection of mammalian cells with retroviral shRNAs.
abstract: Viral infection is a quite simple approach for stably introducing transgenes (e.g., those encoding short-hairpin RNAs [shRNAs]) into the genome. The critical aspects are that the virus and the target cell should be appropriately matched. For example, a virus bearing an ecotropic envelope protein will not infect a human cell line unless the appropriate receptor has been purposefully expressed. VSV-G (vesicular stomatitus virus glycoprotein) pseudotyped viruses have the greatest host range. Nondividing cells can only be infected with lentiviruses, but the additional safety precautions necessary for the use of these tools should dissuade their application to routinely cultured cell lines.
authors.forename: Kenneth
authors.initials: K
authors.lastname: Chang
authors.forename: Krista
authors.initials: K
authors.lastname: Marran
authors.forename: Amy
authors.initials: A
authors.lastname: Valentine
authors.forename: Gregory J
authors.initials: GJ
authors.lastname: Hannon
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D003584:Cytological Techniques / Q000379:methods*; D020868:Gene Silencing; D006801:Humans; D008322:Mammals; D008967:Molecular Biology / Q000379:methods; D034741:RNA, Small Interfering / Q000235:genetics* / Q000378:metabolism*; D012190:Retroviridae / Q000235:genetics*; D014161:Transduction, Genetic
chemical_list: D034741:RNA, Small Interfering