title: Hydrogel micropillars with integrin selective peptidomimetic functionalized nanopatterned tops: a new tool for the measurement of cell traction forces transmitted through αvβ3- or α5β1-integrins.
abstract: Poly(ethylene glycol) micropillars with gold nanopatterns on top are functionalized with two integrin selective ligands. This platform is a powerful new tool to determine the specific contribution of traction forces involved in cell adhesion mediated by α5β1- and αvβ3-integrins. Cells adherent via α5β1-integrins have a tendency to exert higher maximum forces than cells adhering via αvβ3-integrins.
authors.affiliation: Department of New Materials and Biosystems, Max Planck Institute for Intelligent Systems, Heisenbergstr. 3, 70569, Stuttgart, Germany; Department of Biophysical Chemistry, University of Heidelberg, Im Neuenheimer Feld 253, 69120, Heidelberg, Germany.
authors.forename: Sabri
authors.initials: S
authors.lastname: Rahmouni
authors.forename: Aaron
authors.initials: A
authors.lastname: Lindner
authors.forename: Florian
authors.initials: F
authors.lastname: Rechenmacher
authors.forename: Stefanie
authors.initials: S
authors.lastname: Neubauer
authors.forename: Tariq Rashad Ali
authors.initials: TR
authors.lastname: Sobahi
authors.forename: Horst
authors.initials: H
authors.lastname: Kessler
authors.forename: Elisabetta Ada
authors.initials: EA
authors.lastname: Cavalcanti-Adam
authors.forename: Joachim Pius
authors.initials: JP
authors.lastname: Spatz
mesh_terms: D001696:Biomechanical Phenomena; D006046:Gold / Q000737:chemistry; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry; D039081:Integrin alpha5beta1 / Q000378:metabolism*; D039302:Integrin alphaVbeta3 / Q000378:metabolism*; D055595:Mechanical Phenomena; D053768:Metal Nanoparticles / Q000737:chemistry*; D008823:Micelles; D008853:Microscopy; D057786:Peptidomimetics / Q000737:chemistry* / Q000378:metabolism*; D011092:Polyethylene Glycols / Q000737:chemistry*; D013379:Substrate Specificity
chemical_list: D039081:Integrin alpha5beta1; D039302:Integrin alphaVbeta3; D008823:Micelles; D057786:Peptidomimetics; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D011092:Polyethylene Glycols; D006046:Gold
keywords: cell adhesion; integrin; micropillar; peptidomimetics; traction force
references.citation: Biophys J. 2007 Apr 15;92(8):2964-74
references.pmid: 17277192
references.citation: J Cell Sci. 2003 Nov 15;116(Pt 22):4605-13
references.pmid: 14576354
references.citation: Angew Chem Int Ed Engl. 2013 Jan 28;52(5):1572-5
references.pmid: 23345131
references.citation: J Cell Biol. 2002 Dec 23;159(6):1071-86
references.pmid: 12486108
references.citation: Langmuir. 2001 Sep 4;17(18):5440-7
references.pmid: 12448421
references.citation: Nat Rev Mol Cell Biol. 2009 Jan;10(1):21-33
references.pmid: 19197329
references.citation: Trends Cell Biol. 2002 Feb;12(2):79-84
references.pmid: 11849971
references.citation: Adv Mater. 2010 Feb 23;22(8):868-71
references.pmid: 20217807
references.citation: J Struct Biol. 2005 Aug;151(2):182-95
references.pmid: 16043363
references.citation: Chemphyschem. 2004 Mar 19;5(3):383-8
references.pmid: 15067875
references.citation: Chemistry. 2013 Jul 08;19(28):9218-23
references.pmid: 23744802
references.citation: Biophys J. 2002 Sep;83(3):1380-94
references.pmid: 12202364
references.citation: Curr Opin Cell Biol. 2002 Feb;14(1):88-103
references.pmid: 11792550
references.citation: Proc Natl Acad Sci U S A. 2009 Sep 22;106(38):16245-50
references.pmid: 19805288
references.citation: Biomaterials. 2007 Nov;28(32):4739-47
references.pmid: 17697710
references.citation: Biophys J. 2010 Feb 17;98(4):543-51
references.pmid: 20159150
references.citation: Biophys J. 2003 Feb;84(2 Pt 1):1252-62
references.pmid: 12547805
references.citation: Biointerphases. 2011 Mar;6(1):MR1-12
references.pmid: 21428688
references.citation: J Cell Biol. 2005 Oct 24;171(2):209-15
references.pmid: 16247023
references.citation: J Phys Condens Matter. 2010 May 19;22(19):194104
references.pmid: 20523913
references.citation: Nat Methods. 2012 Jun 28;9(7):676-82
references.pmid: 22743772
references.citation: Proc Natl Acad Sci U S A. 2003 Feb 18;100(4):1484-9
references.pmid: 12552122
references.citation: Ann N Y Acad Sci. 2001 Nov;944:62-73
references.pmid: 11797696
references.citation: IUBMB Life. 2009 Jul;61(7):731-8
references.pmid: 19514020
references.citation: Proc Natl Acad Sci U S A. 2012 May 1;109(18):6933-8
references.pmid: 22509005
references.citation: Nat Cell Biol. 2007 Aug;9(8):858-67
references.pmid: 17671451
references.citation: Proc Natl Acad Sci U S A. 2005 Feb 15;102(7):2390-5
references.pmid: 15695588
references.citation: Nat Cell Biol. 1999 Sep;1(5):E131-8
references.pmid: 10559956
references.citation: Nat Methods. 2010 Sep;7(9):733-6
references.pmid: 20676108
references.citation: J Cell Sci. 2001 Jul;114(Pt 14):2553-60
references.pmid: 11683383
references.citation: Nano Lett. 2009 Oct;9(10):3629-34
references.pmid: 19722536
references.citation: Proc Natl Acad Sci U S A. 2012 Apr 3;109(14):5328-33
references.pmid: 22431603
references.citation: Cell Motil Cytoskeleton. 2007 Jul;64(7):509-18
references.pmid: 17342763
references.citation: J Biol Chem. 2001 Sep 14;276(37):34759-67
references.pmid: 11395501