title: Circulating long non-coding RNAs in plasma of patients with gastric cancer.
abstract: BACKGROUND
  We examined the levels of long non-coding RNAs (lncRNAs) in the plasma of patients with gastric cancer to assess their clinical significance for diseases diagnosing and monitoring.
  
  
  METHODS
  We investigated the stability of plasma lncRNAs, and then confirmed the appropriateness of the lncRNA assay with a pre-amplification method. The levels of plasma lncRNAs, H19, HOX antisense intergenic RNA (HOTAIR), and metastasis associated lung adenocarcinoma transcript-1 (MALAT1), were then analyzed in patients with gastric cancer (GC) and healthy controls.
  
  
  RESULTS
  Plasma lncRNAs exhibited minimal gradual instability only under several severe conditions. Analysis showed that samples with pre-amplification had a higher level of linearity in the reverse transcription polymerase chain reaction (RT-PCR) assay than those without pre-amplification. Plasma H19 levels were significantly higher in patients than in healthy controls. Plasma H19 levels were significantly reduced in postoperative samples.
  
  
  CONCLUSIONS
  Circulating lncRNAs can be detectable in plasma, and the detection of circulating lncRNAs may provide new complementary tumor markers for gastric cancer.
authors.affiliation: Division of Digestive Surgery, Department of Surgery, Kyoto Prefectural University of Medicine, 465 Kajii-cho, Kamigyo-ku, Kyoto 6028566, Japan.
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Arita
authors.forename: Daisuke
authors.initials: D
authors.lastname: Ichikawa
authors.forename: Hirotaka
authors.initials: H
authors.lastname: Konishi
authors.forename: Shuhei
authors.initials: S
authors.lastname: Komatsu
authors.forename: Atsushi
authors.initials: A
authors.lastname: Shiozaki
authors.forename: Katsutoshi
authors.initials: K
authors.lastname: Shoda
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Kawaguchi
authors.forename: Shoji
authors.initials: S
authors.lastname: Hirajima
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Nagata
authors.forename: Takeshi
authors.initials: T
authors.lastname: Kubota
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Fujiwara
authors.forename: Kazuma
authors.initials: K
authors.lastname: Okamoto
authors.forename: Eigo
authors.initials: E
authors.lastname: Otsuji
mesh_terms: D016022:Case-Control Studies; D006801:Humans; D011182:Postoperative Care; D011300:Preoperative Care; D020871:RNA Stability; D062085:RNA, Long Noncoding / Q000097:blood*; D013274:Stomach Neoplasms / Q000097:blood* / Q000235:genetics* / Q000601:surgery
chemical_list: C094335:H19 long non-coding RNA; C561566:HOTAIR long untranslated RNA, human; C557041:MALAT1 long non-coding RNA, human; D062085:RNA, Long Noncoding
keywords: Gastric cancer; biomarker; long non-coding RNAs; plasma