title: Choline metabolic profiling by magnetic resonance spectroscopy.
abstract: The revised version of cancer hallmarks, depicting the biological properties acquired during tumor development and progression, includes the capability to modify or reprogram cellular metabolism. High-resolution multinuclear magnetic resonance spectroscopy (MRS) provides noninvasive means of monitoring metabolites that play a central role in several pathways, measuring the rates at which reactions within the pathways take place, and investigating how these rates are controlled in such a way that a metabolic precursor and cofactors are provided according to the demand. Here we describe comprehensive methods for assaying the activity of key enzymes involved in the phosphatidylcholine metabolic pathways responsible for phosphocholine accumulation in ovarian cancer cells using high-resolution (1)H and (31)P MRS analyses of cell lysates or cytosolic preparations.
authors.affiliation: Department of Cell Biology and Neurosciences, Istituto Superiore di Sanità, Rome, Italy.
authors.forename: Egidio
authors.initials: E
authors.lastname: Iorio
authors.forename: Alessandro
authors.initials: A
authors.lastname: Ricci
authors.forename: Maria Elena
authors.initials: ME
authors.lastname: Pisanu
authors.forename: Marina
authors.initials: M
authors.lastname: Bagnoli
authors.forename: Franca
authors.initials: F
authors.lastname: Podo
authors.forename: Silvana
authors.initials: S
authors.lastname: Canevari
mesh_terms: D045744:Cell Line, Tumor; D002794:Choline / Q000378:metabolism*; D005260:Female; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D053858:Metabolic Networks and Pathways / Q000235:genetics; D055442:Metabolome; D010051:Ovarian Neoplasms / Q000378:metabolism* / Q000473:pathology; D010713:Phosphatidylcholines / Q000378:metabolism*
chemical_list: D010713:Phosphatidylcholines; D002794:Choline