title: Assaying hematopoiesis using zebrafish.
abstract: The zebrafish has become a commonly used model for studying hematopoiesis as a result of its unique attributes. Zebrafish are highly suitable for large-scale genetic and chemical screens compared to other vertebrate systems. It is now possible to analyze hematopoietic lineages in zebrafish and validate cell function via transplantation assays. Here, we review advancements over the past decade in forward genetic screens, chemical screens, fluorescence-activated cell sorting analysis, and transplantation assays. Integrating these approaches enables new chemical and genetic screens that assay cell function within the hematopoietic system. Studies in zebrafish will continue to contribute and expand our knowledge about hematopoiesis, and develop novel treatments for clinical applications.
authors.affiliation: Stem Cell Program and Division of Hematology/Oncology, Children's Hospital Boston, Harvard Medical School, Boston, MA, USA.
authors.forename: Sonja
authors.initials: S
authors.lastname: Boatman
authors.forename: Francesca
authors.initials: F
authors.lastname: Barrett
authors.forename: Sruthi
authors.initials: S
authors.lastname: Satishchandran
authors.forename: Lili
authors.initials: L
authors.lastname: Jing
authors.forename: Ilya
authors.initials: I
authors.lastname: Shestopalov
authors.forename: Leonard I
authors.initials: LI
authors.lastname: Zon
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D019070:Cell Lineage; D006410:Hematopoiesis / Q000502:physiology*; D018380:Hematopoietic Stem Cell Transplantation; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000378:metabolism; D006801:Humans; D016130:Immunophenotyping; D010641:Phenotype; D015027:Zebrafish / Q000196:embryology* / Q000502:physiology*
keywords: AGM; ALM; BM; CHT; ENU; FACS; FSC; GVHD; HCT; Hematopoiesis; ICM; MHC; PAF; PG; PLM; Pol II Associated Factor; SSC; Screens; Stem cell; TGFβ; TIF1γ; Transplantation; WKM; Zebrafish; anterior lateral mesoderm; aorta–gonad–mesonephros; bone marrow; caudal hematopoietic tissue; ethylnitrosourea; fluorescence-activated cell sorting; forward light scatter; graft-versus-host-disease; hCB; hematopoietic cellular transplantation; hours post fertilization; hpf; human cord blood; intermediate cell mass; major histocompatibility complex; posterior lateral mesoderm; prostaglandin; side light scatter; transcription intermediary factor1γ; transforming growth factor beta; whole kidney marrow
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