title: [Synthesis of folate receptor-targeted nanoprobe for detection of cancer cells and its spectral analysis].
abstract: Folate receptor (FR) is particularly upregulated in many epithelial cancer cells membrane and limited distribution is found in normal tissues. In the present work, the folic acid protected gold nanoparticles (FA-GNPs) were synthesized by a simple and quick method, in which chloroauric acid (HAuCl4) was reduced by sodium borohydride (NaBH4) in the presence of FA is used as stabilizer. UV-Visible spectroscopy and transmission electron microscopy (TEM) were used to characterize the shape and size distribution of the produced FA-GNPs. X-ray photoelectron spectroscopy and cell experiment were employed to confirm the immobilization of FA and GNPs. The results showed that FA-GNPs have a good size distribution in the 3-5 nm diameter range. Moreover, it is very stable even in solution with high concentration of salt (up to 3.5% NaCl), and even high speed centrifuges of 25 000 r x min(-1) could not cause aggregation. The nanoparticles could be used to detect cancer cells.
authors.affiliation: The Key Laboratory of Biomedical Information Engineering of Ministry of Education, and Institute of Biomedical Analytical Technology and Instrumentation, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710049, China. zsycp@mail.xjtu.edu.cn
authors.forename: Cui-Ping
authors.initials: CP
authors.lastname: Yao
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.forename: Yang
authors.initials: Y
authors.lastname: Yang
authors.forename: Yan-Hua
authors.initials: YH
authors.lastname: Dong
authors.forename: Yang
authors.initials: Y
authors.lastname: Xue
authors.forename: Jian-Sheng
authors.initials: JS
authors.lastname: Mei
authors.forename: Wei-Hui
authors.initials: WH
authors.lastname: Zeng
authors.forename: Zhen-Xi
authors.initials: ZX
authors.lastname: Zhang
mesh_terms: D045744:Cell Line, Tumor; D058973:Folate Receptors, GPI-Anchored / Q000378:metabolism*; D005492:Folic Acid / Q000737:chemistry*; D006046:Gold / Q000737:chemistry*; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry*; D056951:Photoelectron Spectroscopy / Q000379:methods*; D013057:Spectrum Analysis / Q000379:methods
chemical_list: D058973:Folate Receptors, GPI-Anchored; D006046:Gold; D005492:Folic Acid