title: Expression, purification, crystallization and preliminary crystallographic study of FtsA from methicillin-resistant Staphylococcus aureus.
abstract: FtsA from methicillin-resistant Staphylococcus aureus (MRSA) was cloned, overexpressed and purified. The protein was crystallized using the sitting-drop vapour-diffusion technique. A cocrystal with β-γ-imidoadenosine 5'-phosphate (AMPPNP; a nonhydrolysable ATP analogue) was grown using PEG 3350 as a precipitant at 293 K. X-ray diffraction data were collected to a resolution of 2.3 Å at 100 K. The crystal belonged to the monoclinic space group P2₁, with unit-cell parameters a = 75.31, b = 102.78, c = 105.90 Å, β = 96.54°. The calculated Matthews coefficient suggested that the asymmetric unit contained three or four monomers.
authors.affiliation: Department of Applied Chemistry, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan.
authors.forename: Junso
authors.initials: J
authors.lastname: Fujita
authors.forename: Yuma
authors.initials: Y
authors.lastname: Miyazaki
authors.forename: Mika
authors.initials: M
authors.lastname: Hirose
authors.forename: Chioko
authors.initials: C
authors.lastname: Nagao
authors.forename: Eiichi
authors.initials: E
authors.lastname: Mizohata
authors.forename: Yoshimi
authors.initials: Y
authors.lastname: Matsumoto
authors.forename: Kenji
authors.initials: K
authors.lastname: Mizuguchi
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Inoue
authors.forename: Hiroyoshi
authors.initials: H
authors.lastname: Matsumura
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification*; D003460:Crystallization; D018360:Crystallography, X-Ray; D015964:Gene Expression Regulation, Bacterial; D055624:Methicillin-Resistant Staphylococcus aureus; D008969:Molecular Sequence Data
chemical_list: D001426:Bacterial Proteins; C065103:FtsA protein, Bacteria
keywords: FtsA; bacterial divisome; methicillin-resistant Staphylococcus aureus
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