title: Mesoporous iron oxide nanoparticles prepared by polyacrylic acid etching and their application in gene delivery to mesenchymal stem cells.
abstract: Novel monodisperse mesoporous iron oxide nanoparticles (m-IONPs) were synthesized by a postsynthesis etching approach and characterized by electron microscopy. In this approach, solid iron oxide nanoparticles (s-IONPs) were first prepared following a solvothermal method, and then etched anisotropically by polyacrylic acid to form the mesoporous nanostructures. MTT cytotoxicity assay demonstrated that the m-IONPs have good biocompatibility with mesenchymal stem cells (MSCs). Owing to their mesoporous structure and good biocompatibility, these monodisperse m-IONPs were used as a nonviral vector for the delivery of a gene of vascular endothelial growth factor (VEGF) tagged with a green fluorescence protein (GFP) into the hard-to-transfect stem cells. Successful gene delivery and transfection were verified by detecting the GFP fluorescence from MSCs using fluorescence microscopy. Our results illustrated that the m-IONPs synthesized in this work can serve as a potential nonviral carrier in gene therapy where stem cells should be first transfected and then implanted into disease sites for disease treatment.
authors.affiliation: Department of Chemistry and Biochemistry, Stephenson Life Sciences Research Center, University of Oklahoma, Norman, Oklahoma, 73019.
authors.forename: Binrui
authors.initials: B
authors.lastname: Cao
authors.forename: Penghe
authors.initials: P
authors.lastname: Qiu
authors.forename: Chuanbin
authors.initials: C
authors.lastname: Mao
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000378:metabolism*; D002470:Cell Survival / Q000187:drug effects; D005290:Ferric Compounds / Q000378:metabolism*; D018014:Gene Transfer Techniques; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000032:analysis / Q000235:genetics; D008422:Materials Testing; D059630:Mesenchymal Stem Cells / Q000187:drug effects* / Q000502:physiology*; D008854:Microscopy, Electron; D053758:Nanoparticles / Q000378:metabolism* / Q000648:ultrastructure; D051381:Rats; D011993:Recombinant Fusion Proteins / Q000032:analysis / Q000235:genetics; D013194:Staining and Labeling; D013778:Tetrazolium Salts / Q000378:metabolism; D013844:Thiazoles / Q000378:metabolism; D014162:Transfection; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics
chemical_list: D001672:Biocompatible Materials; D005290:Ferric Compounds; D011993:Recombinant Fusion Proteins; D013778:Tetrazolium Salts; D013844:Thiazoles; D042461:Vascular Endothelial Growth Factor A; D049452:Green Fluorescent Proteins; C000499:ferric oxide; C022616:thiazolyl blue
keywords: gene delivery; iron oxide nanoparticles; mesoporous nanostructures; postsynthesis etching
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