title: Sequence polymorphisms of rfbT among the Vibrio cholerae O1 strains in the Ogawa and Inaba serotype shifts.
abstract: BACKGROUND
  Vibrio cholerae serogroup O1 has two major serotypes, Ogawa and Inaba, which may alternate among cholera epidemics. The rfbT gene is responsible for the conversion between the two serotypes. In this study, we surveyed the sequence variance of rfbT in the Ogawa and Inaba strains in China over a 48-year (1961-2008) period in which serotype shifts occurred among epidemic years.
  
  
  RESULTS
  Various mutation events including single nucleotide, short fragment insertions/deletions and transposases insertions, were found in the rfbT gene of the Inaba strains. Ectopically introducing an intact rfbT could overcome the mutations by converting the Inaba serotype to the Ogawa serotype, suggesting the effects of these mutations on the function of RfbT. Characteristic rfbT mutations were recognized in the Inaba strains among Inaba serotype dominant epidemic years which were separate from the Ogawa dominant epidemics. Three distinguishable mutation sites in rfbT between the classical and the El Tor biotype strains were identified and could serve as biotype-specific biomarkers.
  
  
  CONCLUSIONS
  Our results provide a comprehensive picture of the rfbT gene mutations among the V. cholerae O1 strains in different epidemic periods, which could be further used as the tracing markers in clonality analysis and dissemination surveillance of the epidemic strains.
authors.affiliation: State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, 102206, People's Republic of China.
authors.forename: Weili
authors.initials: W
authors.lastname: Liang
authors.forename: Luxi
authors.initials: L
authors.lastname: Wang
authors.forename: Pu
authors.initials: P
authors.lastname: Liang
authors.forename: Xiao
authors.initials: X
authors.lastname: Zheng
authors.forename: Haijian
authors.initials: H
authors.lastname: Zhou
authors.forename: Jingyun
authors.initials: J
authors.lastname: Zhang
authors.forename: Lijuan
authors.initials: L
authors.lastname: Zhang
authors.forename: Biao
authors.initials: B
authors.lastname: Kan
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D002681:China; D002771:Cholera / Q000382:microbiology; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA; D012703:Serotyping; D041241:Vibrio cholerae O1 / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; C082559:RfbT protein, Vibrio cholerae
references.citation: Microbiol Immunol. 1993;37(4):281-8
references.pmid: 7688846
references.citation: Infect Immun. 1979 Jun;24(3):974-8
references.pmid: 468386
references.citation: Bull World Health Organ. 1966;34(2):161-95
references.pmid: 5328492
references.citation: PLoS One. 2012;7(5):e37283
references.pmid: 22662140
references.citation: Foodborne Pathog Dis. 2006 Spring;3(1):51-8
references.pmid: 16602979
references.citation: J Microbiol Methods. 2011 Nov;87(2):208-12
references.pmid: 21871929
references.citation: PLoS One. 2008;3(12):e4053
references.pmid: 19115014
references.citation: Microbiol Mol Biol Rev. 1998 Dec;62(4):1301-14
references.pmid: 9841673
references.citation: Am J Trop Med Hyg. 2012 Jan;86(1):36-8
references.pmid: 22232448
references.citation: Infect Immun. 2003 Oct;71(10):5498-504
references.pmid: 14500467
references.citation: J Med Microbiol. 2004 Nov;53(Pt 11):1105-1107
references.pmid: 15496388
references.citation: Mol Microbiol. 1996 Feb;19(4):815-26
references.pmid: 8820651
references.citation: FEBS Lett. 1991 Jul 29;286(1-2):159-62
references.pmid: 1713857
references.citation: Proc Biol Sci. 2006 Nov 22;273(1603):2879-86
references.pmid: 17015366
references.citation: J Clin Microbiol. 1993 Jan;31(1):22-5
references.pmid: 7678018
references.citation: Infect Immun. 2000 Nov;68(11):6391-7
references.pmid: 11035750
references.citation: Ann Biochem Exp Med. 1963 Dec;23:523-30
references.pmid: 14154194
references.citation: Biochem Biophys Res Commun. 1993 Jan 15;190(1):302-7
references.pmid: 8422256
references.citation: J Gen Microbiol. 1957 Jun;16(3):721-9
references.pmid: 13439157
references.citation: J Infect Dis. 2002 Jul 15;186(2):246-51
references.pmid: 12134262
references.citation: DNA Cell Biol. 2004 Nov;23(11):723-41
references.pmid: 15585131
references.citation: East Mediterr Health J. 2008 May-Jun;14(3):564-70
references.pmid: 18720620
references.citation: Lancet. 1993 Mar 13;341(8846):704
references.pmid: 8095621
references.citation: Lancet. 1998 Aug 8;352(9126):457-8
references.pmid: 9708764
references.citation: Int J Antimicrob Agents. 2012 Oct;40(4):361-4
references.pmid: 22867881
references.citation: Lett Appl Microbiol. 2012 Apr;54(4):267-71
references.pmid: 22225492
references.citation: J Exp Med. 1988 Oct 1;168(4):1487-92
references.pmid: 2902187
references.citation: J Bacteriol. 2010 Jul;192(13):3524-33
references.pmid: 20348258
references.citation: Microbiology (Reading). 2002 Jun;148(Pt 6):1655-1666
references.pmid: 12055286
references.citation: Proc Biol Sci. 2005 May 7;272(1566):971-7
references.pmid: 16024354
references.citation: JAMA. 1966 Aug 1;197(5):321-6
references.pmid: 5952839
references.citation: Biochim Biophys Acta. 2003 Oct 15;1639(2):65-79
references.pmid: 14559113
references.citation: Clin Microbiol Rev. 1995 Jan;8(1):48-86
references.pmid: 7704895
references.citation: FEMS Microbiol Rev. 2002 Jun;26(2):125-39
references.pmid: 12069878
references.citation: Am J Epidemiol. 1972 Nov;96(5):342-51
references.pmid: 4564735
references.citation: Proc Natl Acad Sci U S A. 1992 Apr 1;89(7):2566-70
references.pmid: 1372980
references.citation: Biomed Environ Sci. 2011 Dec;24(6):579-92
references.pmid: 22365393
references.citation: Emerg Infect Dis. 2011 Nov;17(11):2113-21
references.pmid: 22099115
references.citation: Nature. 2000 Aug 3;406(6795):477-83
references.pmid: 10952301
references.citation: Biomed Environ Sci. 1995 Dec;8(4):350-8
references.pmid: 8719177
references.citation: J Bacteriol. 1969 Sep;99(3):688-95
references.pmid: 5370274
references.citation: J Clin Microbiol. 2006 Sep;44(9):3391-3
references.pmid: 16954282
references.citation: Lancet. 1993 Mar 13;341(8846):703-4
references.pmid: 8095620
references.citation: Gene. 1989 Dec 21;85(1):227-31
references.pmid: 2576015
references.citation: Carbohydr Res. 1994 Mar 18;256(1):113-28
references.pmid: 8194067