title: Chiral magnetic microspheres purified by centrifugal field flow fractionation and microspheres magnetic chiral chromatography for benzoin racemate separation.
abstract: Separation of enantiomers still remains a challenge due to their identical physical and chemical properties in a chiral environment, and the research on specific chiral selector along with separation techniques continues to be conducted to resolve individual enantiomers. In our laboratory the promising magnetic chiral microspheres Fe3O4@SiO2@cellulose-2, 3-bis (3,5-dimethylphenylcarbamate) have been developed to facilitate the resolution using both its magnetic property and chiral recognition ability. In our present studies this magnetic chiral selector was first purified by centrifuge field flow fractionation, and then used to separate benzoin racemate by a chromatographic method. Uniform-sized and masking-impurity-removed magnetic chiral selector was first obtained by field flow fractionation with ethanol through a spiral column mounted on the type-J planetary centrifuge, and using the purified magnetic chiral selector, the final chromatographic separation of benzoin racemate was successfully performed by eluting with ethanol through a coiled tube (wound around the cylindrical magnet to retain the magnetic chiral selector as a stationary phase) submerged in dry ice. In addition, an external magnetic field facilitates the recycling of the magnetic chiral selector.
authors.affiliation: State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, 15 Beisanhuan East Road, Chaoyang District, Beijing 100029, PR China.
authors.forename: Ailin
authors.initials: A
authors.lastname: Tian
authors.forename: Jing
authors.initials: J
authors.lastname: Qi
authors.forename: Yating
authors.initials: Y
authors.lastname: Liu
authors.forename: Fengkang
authors.initials: F
authors.lastname: Wang
authors.forename: Yoichiro
authors.initials: Y
authors.lastname: Ito
authors.forename: Yun
authors.initials: Y
authors.lastname: Wei
mesh_terms: D001573:Benzoin / Q000737:chemistry / Q000302:isolation & purification*; D002498:Centrifugation; D002853:Chromatography, Liquid / Q000379:methods*; D031521:Fractionation, Field Flow; D046529:Microscopy, Electron, Transmission; D008863:Microspheres; D013237:Stereoisomerism
chemical_list: D001573:Benzoin
keywords: Benzoin racemate; Centrifugal field flow fractionation; Chiral magnetic microspheres; Microspheres magnetic chrial chromatography
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