title: Microfabrication, surface modification, and laser guidance techniques to create a neuron biochip.
abstract: In this report we illustrate our application of soft lithography-based microfabrication, surface modification, and our unique laser cell-patterning system toward the creation of neuron biochips. We deposited individual forebrain neurons from Day 7 embryonic chicks into two rows of eight in a silicon microstructure aligned over a microelectrode array (MEA). The polydimethylsiloxane (PDMS) membrane with microstructures to confine cells and guide network connectivity was aligned to the electrodes of a MEA. Both the MEA and the PDMS membrane were treated with O2plasma, Poly-L-Lysine, and Laminin to aid in cell attachment and survival. The primary advantage of our process is that it is quicker and simpler than previous cell-placement methods and may make highly defined neuronal network biochips more practical.
authors.affiliation: Department of Bioengineering and Center for Optical Materials Science and Engineering Technologies, Clemson University, Clemson, SC 29634, USA.
authors.forename: Russell Kirk
authors.initials: RK
authors.lastname: Pirlo
authors.forename: Xiang
authors.initials: X
authors.lastname: Peng
authors.forename: Xiaocong
authors.initials: X
authors.lastname: Yuan
authors.forename: Bruce Zhi
authors.initials: BZ
authors.lastname: Gao
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