title: Evaluation of anti-nociceptive and anti-inflammatory activities of a heterofucan from Dictyota menstrualis.
abstract: Fucan is a term that defines a family of homo- and hetero-polysaccharides containing sulfated l-fucose in its structure. In this work, a heterofucan (F2.0v) from the seaweed, Dictyota menstrualis, was evaluated as an antinociceptive and anti-inflammatory agent. F2.0v (20.0 mg/kg) inhibits 100% of leukocyte migration into the peritoneal cavity after chemical stimulation. However, F2.0v does not alter the expression of interleukin-1 beta (IL-1β) and interleukin-6 (IL-6), as well as tumor necrosis factor alpha (TNF-α). F2.0v (20.0 mg/kg) has peripheral antinociceptive activity with potency similar to dipyrone. On the other hand, it had no effect on pain response on the hot plate test. Confocal microscopy analysis and flow cytometry showed that F2.0v binds to the surface of leucocytes, which leads us to suggest that the mechanism of action of anti-inflammatory and antinociceptive F2.0v is related to its ability to inhibit the migration of leukocytes to the site of tissue injury. In summary, the data show that F2.0v compound has great potential as an antinociceptive and anti-inflammatory, and future studies will be performed to further characterize the mechanism of action of F2.0v.
authors.affiliation: Laboratory of Biotechnology of Natural Polymers (BIOPOL), Department of Biochemistry, Federal University of Rio Grande do Norte (UFRN), Natal-RN 59078-970, Brazil. ivanruilopes@gmail.com
authors.forename: Ivan Rui Lopes
authors.initials: IR
authors.lastname: Albuquerque
authors.forename: Sara Lima
authors.initials: SL
authors.lastname: Cordeiro
authors.forename: Dayanne Lopes
authors.initials: DL
authors.lastname: Gomes
authors.forename: Juliana Luporini
authors.initials: JL
authors.lastname: Dreyfuss
authors.forename: Luciana Guimarães Alves
authors.initials: LG
authors.lastname: Filgueira
authors.forename: Edda Lisboa
authors.initials: EL
authors.lastname: Leite
authors.forename: Helena Bonciani
authors.initials: HB
authors.lastname: Nader
authors.forename: Hugo Alexandre Oliveira
authors.initials: HA
authors.lastname: Rocha
mesh_terms: D000700:Analgesics / Q000302:isolation & purification / Q000494:pharmacology*; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000302:isolation & purification / Q000494:pharmacology*; D002465:Cell Movement / Q000187:drug effects; D004177:Dipyrone / Q000494:pharmacology; D004195:Disease Models, Animal; D005434:Flow Cytometry; D007249:Inflammation / Q000188:drug therapy / Q000503:physiopathology; D007962:Leukocytes / Q000187:drug effects; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D018613:Microscopy, Confocal; D010146:Pain / Q000188:drug therapy / Q000503:physiopathology; D000459:Phaeophyceae / Q000737:chemistry*; D011134:Polysaccharides / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D000700:Analgesics; D000893:Anti-Inflammatory Agents; D011134:Polysaccharides; D004177:Dipyrone; C007789:fucoidan
references.citation: Pharmacol Rev. 2001 Dec;53(4):597-652
references.pmid: 11734620
references.citation: Int J Biol Macromol. 2010 Jan 1;46(1):59-66
references.pmid: 19836415
references.citation: Carbohydr Res. 2003 Jan 20;338(2):153-65
references.pmid: 12526839
references.citation: Phytother Res. 2004 Jul;18(7):551-5
references.pmid: 15305315
references.citation: Biomed Pharmacother. 2010 Jan;64(1):21-8
references.pmid: 19766438
references.citation: Mar Drugs. 2011 Feb 08;9(2):196-223
references.pmid: 21566795
references.citation: Biochem J. 1962 Jul;84:106-10
references.pmid: 13886865
references.citation: Braz J Med Biol Res. 2004 Feb;37(2):167-71
references.pmid: 14762570
references.citation: Planta Med. 2005 Jul;71(7):628-33
references.pmid: 16041648
references.citation: Planta Med. 2008 Jun;74(7):712-8
references.pmid: 18496786
references.citation: J Pharmacol Exp Ther. 1953 Mar;107(3):385-93
references.pmid: 13035677
references.citation: J Biol Chem. 2005 Dec 16;280(50):41278-88
references.pmid: 16174777
references.citation: Anal Biochem. 1978 May;86(1):142-6
references.pmid: 655375
references.citation: Mar Drugs. 2011;9(6):952-66
references.pmid: 21747741
references.citation: Int Immunopharmacol. 2011 Sep;11(9):1241-50
references.pmid: 21504801
references.citation: Phytochemistry. 2010 Sep;71(13):1586-95
references.pmid: 20599236
references.citation: J Appl Toxicol. 2010 Oct;30(7):708-15
references.pmid: 20589741
references.citation: J Biomed Mater Res. 2002 Jun 5;60(3):375-83
references.pmid: 11920660
references.citation: Int J Mol Sci. 2011;12(5):3352-65
references.pmid: 21686189
references.citation: Bioorg Med Chem. 2008 Nov 1;16(21):9588-95
references.pmid: 18835720
references.citation: Nat Prod Commun. 2011 Jun;6(6):863-6
references.pmid: 21815427
references.citation: Biol Pharm Bull. 2008 Apr;31(4):691-5
references.pmid: 18379064
references.citation: New Phytol. 2010 Oct;188(1):82-97
references.pmid: 20618907
references.citation: Anal Biochem. 1976 Feb;70(2):645-7
references.pmid: 131498
references.citation: Basic Clin Pharmacol Toxicol. 2012 Apr;110(4):335-41
references.pmid: 21985563
references.citation: Eur J Pharmacol. 2000 Jan 3;387(1):111-8
references.pmid: 10633169
references.citation: Appl Biochem Biotechnol. 2011 Jul;164(6):841-50
references.pmid: 21302149
references.citation: Planta Med. 2005 Apr;71(4):379-81
references.pmid: 15856421
references.citation: Mar Drugs. 2011 Jan 24;9(1):124-38
references.pmid: 21339951