title: RNA binding of T-cell intracellular antigen-1 (TIA-1) C-terminal RNA recognition motif is modified by pH conditions.
abstract: T-cell intracellular antigen-1 (TIA-1) is a DNA/RNA-binding protein that regulates critical events in cell physiology by the regulation of pre-mRNA splicing and mRNA translation. TIA-1 is composed of three RNA recognition motifs (RRMs) and a glutamine-rich domain and binds to uridine-rich RNA sequences through its C-terminal RRM2 and RRM3 domains. Here, we show that RNA binding mediated by either isolated RRM3 or the RRM23 construct is controlled by slight environmental pH changes due to the protonation/deprotonation of TIA-1 RRM3 histidine residues. The auxiliary role of the C-terminal RRM3 domain in TIA-1 RNA recognition is poorly understood, and this work provides insight into its binding mechanisms.
authors.affiliation: From the Instituto de Bioquímica Vegetal y Fotosíntesis (IBVF), Centro de Investigaciones Científicas Isla de la Cartuja (cicCartuja), Universidad de Sevilla-Consejo Superior de Investigaciones Científicas (CSIC), 41092 Seville, Spain and.
authors.forename: Isabel
authors.initials: I
authors.lastname: Cruz-Gallardo
authors.affiliation: From the Instituto de Bioquímica Vegetal y Fotosíntesis (IBVF), Centro de Investigaciones Científicas Isla de la Cartuja (cicCartuja), Universidad de Sevilla-Consejo Superior de Investigaciones Científicas (CSIC), 41092 Seville, Spain and.
authors.forename: Ángeles
authors.initials: Á
authors.lastname: Aroca
authors.affiliation: the Swedish NMR Centre, University of Gothenburg, SE-40530 Gothenburg, Sweden.
authors.forename: Cecilia
authors.initials: C
authors.lastname: Persson
authors.affiliation: the Swedish NMR Centre, University of Gothenburg, SE-40530 Gothenburg, Sweden.
authors.forename: B Göran
authors.initials: BG
authors.lastname: Karlsson
authors.affiliation: From the Instituto de Bioquímica Vegetal y Fotosíntesis (IBVF), Centro de Investigaciones Científicas Isla de la Cartuja (cicCartuja), Universidad de Sevilla-Consejo Superior de Investigaciones Científicas (CSIC), 41092 Seville, Spain and. Electronic address: idiazmoreno@us.es.
authors.forename: Irene
authors.initials: I
authors.lastname: Díaz-Moreno
mesh_terms: D020816:Amino Acid Motifs; D006801:Humans; D006863:Hydrogen-Ion Concentration; D039102:Poly(A)-Binding Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D014176:Protein Biosynthesis / Q000502:physiology; D017434:Protein Structure, Tertiary; D012326:RNA Splicing / Q000502:physiology; D012333:RNA, Messenger / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D000074019:T-Cell Intracellular Antigen-1
chemical_list: D039102:Poly(A)-Binding Proteins; D012333:RNA, Messenger; D000074019:T-Cell Intracellular Antigen-1; C071558:TIA1 protein, human
keywords: Circular Dichroism (CD); Fluorescence; NMR; Protein/Nucleic Acid Interaction; RNA Recognition Motif; RNA-binding Proteins; T-cell Intracellular Antigen-1 (TIA-1)
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