title: Bacillus borbori sp. Nov., isolated from an electrochemically active biofilm.
abstract: A Gram-positive, facultative anaerobic, motile, endospore-forming rod strain, designated DX-4(T), was isolated from an electrochemically active biofilm. Growth occurred at 30-65 °C (optimum 55 °C), at pH 6.0-8.5 (optimum pH 7.0-7.5) and with <6 % (w/v) NaCl. Cells were catalase- and oxidase-positive. The main respiratory quinone was MK-7, the predominant polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol mannoside, and unidentified aminophospholipid, the DNA G+C content was 38.6 mol% and the major fatty acids (>5 %) were iso-C15:0 (38.9 %), iso-C17:0 (30.5 %), iso-C16:0 (5.6 %), and anteiso-C17:0 (5.2 %). The phylogenetic analysis based on 16S rRNA gene sequence comparisons revealed that strain DX-4(T) is a member of the genus Bacillus. The results of phenotypic, chemotaxonomic, and genotypic analyses clearly indicated that strain DX-4(T) represents a novel species, for which the name Bacillus borbori sp. nov. is proposed. The type strain is DX-4(T) (= CCTCC AB2012196(T) = KCTC 33103(T)).
authors.affiliation: Guangdong Institute of Eco-Environmental and Soil Sciences, Guangzhou, 510650, People's Republic of China.
authors.forename: Yue-Qiang
authors.initials: YQ
authors.lastname: Wang
authors.forename: Yong
authors.initials: Y
authors.lastname: Yuan
authors.forename: Zhen
authors.initials: Z
authors.lastname: Yu
authors.forename: Gui-Qin
authors.initials: GQ
authors.lastname: Yang
authors.forename: Shun-Gui
authors.initials: SG
authors.lastname: Zhou
mesh_terms: D000332:Aerobiosis; D001407:Bacillus / Q000302:isolation & purification* / Q000502:physiology*; D015373:Bacterial Typing Techniques; D001482:Base Composition; D018441:Biofilms; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D008124:Locomotion; D008853:Microscopy; D008969:Molecular Sequence Data; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D011809:Quinones / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012722:Sewage / Q000382:microbiology; D012965:Sodium Chloride / Q000378:metabolism; D013696:Temperature
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D010743:Phospholipids; D011809:Quinones; D012336:RNA, Ribosomal, 16S; D012722:Sewage; D012965:Sodium Chloride
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