title: Hepatoprotective activity of Calotropis gigantea flowers against carbon-tetrachloride-induced liver damage in mice.
abstract: Ethanol extract of Calotropis gigantea flowers (CGFE) was evaluated for its antioxidant and hepatoprotective activity to validate its use in traditional therapeutic indications. This CGFE exhibited significant antioxidant activity (at 20, 40, 60, 80 and 100 µg/ml in vitro) as evidenced by its hydroxyl, nitric oxide and hydrogen peroxide anion radical scavenging activities. This in vitro antioxidant activity was reinforced by a significant hepatoprotection (at 250 and 500 mg/kg dose) by decreasing the activity of serum glutamate oxaloacetate transaminase and serum glutamate pyruvate transaminase. The hepatoprotective activity of the CGFE was comparable with standard drug silymarin (100 mg/kg, p.o.).The results obtained from present study indicate the presence of natural antioxidants and hepatoprotective constituents. Hence, the above finding confirms in vitro antioxidant and hepatoprotective potential of CGFE in mice.
authors.affiliation: Department of Pharmacology, Sinhgad Institute of Pharmaceutical Sciences, Lonavala 410401, India. deepa_ingawale@yahoo.com
authors.forename: Deepa Krushna
authors.initials: DK
authors.lastname: Ingawale
authors.forename: Satish Karbhari
authors.initials: SK
authors.lastname: Mandlik
authors.forename: Ajay Digambar
authors.initials: AD
authors.lastname: Kshirsagar
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology / Q000627:therapeutic use*; D001219:Aspartate Aminotransferases / Q000097:blood; D031107:Calotropis; D056486:Chemical and Drug Induced Liver Injury / Q000097:blood / Q000188:drug therapy*; D005260:Female; D035264:Flowers; D005609:Free Radicals / Q000378:metabolism; D008099:Liver / Q000187:drug effects* / Q000201:enzymology; D008297:Male; D008519:Medicine, Traditional; D051379:Mice; D008815:Mice, Inbred Strains; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use*; D012838:Silymarin / Q000494:pharmacology
chemical_list: D000975:Antioxidants; D005609:Free Radicals; D010936:Plant Extracts; D012838:Silymarin; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase