title: New methodological approach to improve reproducibility of brachial artery flow-mediated dilatation.
abstract: BACKGROUND
  Measurement of flow-mediated dilation (FMD) is well known as a noninvasive method for an assessment of vascular endothelial function. However, the reproducibility is a major issue of FMD measurement. The purpose of this study is to examine the reproducibility of the new FMD measurement with medial epicondyle method.
  
  
  METHODS
  First, to evaluate the variability of the brachial artery diameter, 23 volunteers recruited from 32 healthy volunteers were examined for a brachial artery diameter at rest using with FMD equipment. Second, to evaluate the reproducibility of the FMD measurement, all volunteers underwent the FMD measurement, which was repeated at 2-week interval using the traditional method and the medial epicondyle method. The reproducibility in both methods was evaluated by 2 independent observers who measured on the same subject to assess the inter-observer reproducibility, and 1 observer who measured the same subject twice to assess the intra-observer reproducibility regarding the baseline value of arterial diameter and FMD.
  
  
  RESULTS
  The variability of brachial artery diameter was 0.57 ± 0.27 mm in 23 healthy volunteers. In the study of inter- and intra-observer reproducibility, 2 parameters including intra-class correlation coefficient and Pearson's correlation coefficient by medial epicondyle method are superior to those by traditional method.
  
  
  CONCLUSIONS
  These results suggest that medial epicondyle methodological approach to measure FMD is superior to traditional method.
authors.affiliation: Department of Medical Laboratory Science, Faculty of Health Sciences, Junshin Gakuen University, Fukuoka, Japan.
authors.forename: Masaaki
authors.initials: M
authors.lastname: Kanahara
authors.forename: Haruhito
authors.initials: H
authors.lastname: Harada
authors.forename: Atsushi
authors.initials: A
authors.lastname: Katoh
authors.forename: Hisao
authors.initials: H
authors.lastname: Ikeda
mesh_terms: D000328:Adult; D000465:Algorithms; D059925:Anatomic Landmarks / Q000000981:diagnostic imaging*; D001783:Blood Flow Velocity / Q000502:physiology; D001916:Brachial Artery / Q000000981:diagnostic imaging* / Q000502:physiology*; D004452:Echocardiography / Q000379:methods*; D004730:Endothelium, Vascular / Q000000981:diagnostic imaging*; D005260:Female; D006801:Humans; D007089:Image Enhancement / Q000379:methods*; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D008297:Male; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014655:Vascular Resistance / Q000502:physiology
keywords: flow-mediated dilatation; reproducibility of brachial artery