title: Modified expression of an auxin-responsive rice CC-type glutaredoxin gene affects multiple abiotic stress responses.
abstract: Glutaredoxins (GRXs) are the ubiquitous oxidoreductase enzymes, which play an important role in defense against various stresses. Here, we report the role of a CC-type GRX gene from rice, OsGRX8, in abiotic stress tolerance. OsGRX8 protein was found to be localized in nucleus and cytosol and its gene expression is induced by various stress conditions and plant hormone auxin. The over-expression of OsGRX8 in Arabidopsis plants conferred reduced sensitivity to auxin and stress hormone, abscisic acid. In addition, the transgenic Arabidopsis plants exhibited enhanced tolerance to various abiotic stresses, including salinity, osmotic and oxidative stress. Further, the transgenic RNAi rice plants exhibited increased susceptibility to various abiotic stresses, which further confirmed the role of OsGRX8 in abiotic stress responses. The microarray data analysis revealed that expression of a large number of auxin-responsive, known stress-associated and transcription factor encoding genes was altered in GRX transgenic Arabidopsis plants in response to exogenous auxin and stress conditions as compared to wild-type plants. Altogether, these findings suggest the role of OsGRX8 in regulating abiotic stress response and may be used to engineer stress tolerance in crop plants.
authors.affiliation: Functional and Applied Genomics Laboratory, National Institute of Plant Genome Research (NIPGR), Aruna Asaf Ali Marg, New Delhi, 110067, India.
authors.forename: Raghvendra
authors.initials: R
authors.lastname: Sharma
authors.forename: Pushp
authors.initials: P
authors.lastname: Priya
authors.forename: Mukesh
authors.initials: M
authors.lastname: Jain
mesh_terms: D000040:Abscisic Acid / Q000494:pharmacology; D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics / Q000254:growth & development; D018076:DNA, Complementary / Q000235:genetics; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D017343:Genes, Plant / Q000235:genetics*; D054477:Glutaredoxins / Q000235:genetics* / Q000378:metabolism; D007210:Indoleacetic Acids / Q000494:pharmacology*; D020411:Oligonucleotide Array Sequence Analysis; D012275:Oryza / Q000187:drug effects / Q000235:genetics* / Q000502:physiology*; D018384:Oxidative Stress / Q000187:drug effects / Q000235:genetics; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D030821:Plants, Genetically Modified; D021381:Protein Transport / Q000187:drug effects; D034622:RNA Interference; D036226:Seedlings / Q000187:drug effects; D013312:Stress, Physiological / Q000187:drug effects / Q000235:genetics*; D013347:Subcellular Fractions / Q000187:drug effects / Q000378:metabolism
chemical_list: D018076:DNA, Complementary; D054477:Glutaredoxins; D007210:Indoleacetic Acids; D010940:Plant Proteins; D000040:Abscisic Acid
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