title: Microtubule severing by the katanin complex is activated by PPFR-1-dependent MEI-1 dephosphorylation.
abstract: Katanin is an evolutionarily conserved microtubule (MT)-severing complex implicated in multiple aspects of MT dynamics. In Caenorhabditis elegans, the katanin homologue MEI-1 is required for meiosis, but must be inactivated before mitosis. Here we show that PPFR-1, a regulatory subunit of a trimeric protein phosphatase 4 complex, enhanced katanin MT-severing activity during C. elegans meiosis. Loss of ppfr-1, similarly to the inactivation of MT severing, caused a specific defect in meiosis II spindle disassembly. We show that a fraction of PPFR-1 was degraded after meiosis, contributing to katanin inactivation. PPFR-1 interacted with MEL-26, the substrate recognition subunit of the CUL-3 RING E3 ligase (CRL3(MEL-26)), which also targeted MEI-1 for post-meiotic degradation. Reversible protein phosphorylation of MEI-1 may ensure temporal activation of the katanin complex during meiosis, whereas CRL3(MEL-26)-mediated degradation of both MEI-1 and its activator PPFR-1 ensure efficient katanin inactivation in the transition to mitosis.
authors.affiliation: Institut Jacques Monod, Centre National de la Recherche Scientifique, UMR 7592, University of Paris Diderot, F-75205 Paris, France.
authors.forename: José-Eduardo
authors.initials: JE
authors.lastname: Gomes
authors.forename: Nicolas
authors.initials: N
authors.lastname: Tavernier
authors.forename: Bénédicte
authors.initials: B
authors.lastname: Richaudeau
authors.forename: Etienne
authors.initials: E
authors.lastname: Formstecher
authors.forename: Thomas
authors.initials: T
authors.lastname: Boulin
authors.forename: Paul E
authors.initials: PE
authors.lastname: Mains
authors.forename: Julien
authors.initials: J
authors.lastname: Dumont
authors.forename: Lionel
authors.initials: L
authors.lastname: Pintard
mesh_terms: D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000818:Animals; D017173:Caenorhabditis elegans / Q000235:genetics / Q000378:metabolism*; D029742:Caenorhabditis elegans Proteins / Q000378:metabolism*; D000074201:Katanin; D008870:Microtubules / Q000378:metabolism*; D046912:Multiprotein Complexes / Q000378:metabolism; D010749:Phosphoprotein Phosphatases / Q000378:metabolism*; D010766:Phosphorylation
chemical_list: D029742:Caenorhabditis elegans Proteins; D046912:Multiprotein Complexes; C584547:PPFR-1 protein, C elegans; D010749:Phosphoprotein Phosphatases; D000251:Adenosine Triphosphatases; C086678:MEI-1 protein, C elegans; D000074201:Katanin
references.citation: Genome Res. 2005 Mar;15(3):376-84
references.pmid: 15710747
references.citation: Curr Biol. 2006 Oct 10;16(19):1944-9
references.pmid: 17027492
references.citation: Genetics. 1990 Nov;126(3):593-605
references.pmid: 2249759
references.citation: Nature. 2003 Sep 18;425(6955):316-21
references.pmid: 13679922
references.citation: Cell. 2011 Dec 9;147(6):1397-407
references.pmid: 22153081
references.citation: Curr Biol. 2003 May 27;13(11):911-21
references.pmid: 12781129
references.citation: Genes Dev. 2000 May 1;14(9):1072-84
references.pmid: 10809666
references.citation: Genetics. 1974 May;77(1):71-94
references.pmid: 4366476
references.citation: Dev Cell. 2009 Dec;17(6):900-7
references.pmid: 20059959
references.citation: Science. 2002 Feb 15;295(5558):1294-8
references.pmid: 11847342
references.citation: Cell. 1993 Nov 5;75(3):419-29
references.pmid: 8221885
references.citation: Nat Cell Biol. 2009 Mar;11(3):269-77
references.pmid: 19219036
references.citation: PLoS One. 2011 Mar 01;6(3):e16644
references.pmid: 21390299
references.citation: J Cell Biol. 1994 Jul;126(1):199-209
references.pmid: 8027178
references.citation: Trends Cell Biol. 2012 May;22(5):241-9
references.pmid: 22480579
references.citation: Nat Cell Biol. 2003 Nov;5(11):1001-7
references.pmid: 14528312
references.citation: Methods Enzymol. 1995;255:331-42
references.pmid: 8524119
references.citation: Nat Cell Biol. 2010 Sep;12(9):894-901
references.pmid: 20729837
references.citation: Mol Biol Cell. 2011 May;22(9):1550-60
references.pmid: 21372175
references.citation: Dev Biol. 1986 Feb;113(2):449-60
references.pmid: 3949074
references.citation: J Mol Biol. 2002 Nov 1;323(4):763-70
references.pmid: 12419263
references.citation: J Cell Biol. 2006 Dec 18;175(6):881-91
references.pmid: 17178907
references.citation: Cell. 1998 Apr 17;93(2):277-87
references.pmid: 9568719
references.citation: Nature. 2001 Jan 11;409(6817):211-5
references.pmid: 11196647
references.citation: Nature. 2003 Sep 18;425(6955):311-6
references.pmid: 13679921
references.citation: Genome Biol. 2001;2(1):RESEARCH0002
references.pmid: 11178279
references.citation: Genetics. 1998 Sep;150(1):119-28
references.pmid: 9725834
references.citation: Dev Biol. 2003 Aug 1;260(1):245-59
references.pmid: 12885567
references.citation: Curr Opin Cell Biol. 2011 Feb;23(1):78-84
references.pmid: 20708397
references.citation: Mol Biol Cell. 2004 Jan;15(1):142-50
references.pmid: 14565976
references.citation: Genetics. 2009 Mar;181(3):933-43
references.pmid: 19087961
references.citation: Curr Biol. 2006 Jan 10;16(1):56-62
references.pmid: 16338136
references.citation: Nat Genet. 1997 Jul;16(3):277-82
references.pmid: 9207794