title: Dynamics of exocyclic groups in the Escherichia coli O91 O-antigen polysaccharide in solution studied by carbon-13 NMR relaxation.
abstract: Carbon-13 relaxation data are reported for exocyclic groups of hexopyranosyl sugar residues in the repeating unit within the Escherichia coli O91 O-antigen polysaccharide in a dilute D2O solution. The measurements of T 1, T 2 and heteronuclear nuclear Overhauser enhancements were carried out at 310 K at two magnetic fields (16.4 T, 21.1 T). The data were analyzed using the standard and extended Lipari-Szabo models, as well as a conformational jump model. The extended version of the Lipari-Szabo and the two-site jump models were most successful for the hydroxymethyl groups of Gal and GlcNAc sugar residues. Different dynamics was found for the hydroxymethyl groups associated with different configurations (D-gluco, D-galacto) of the sugar residues, the latter being faster than the former.
authors.affiliation: Arrhenius Laboratory, Department of Materials and Environmental Chemistry, Stockholm University, 106 91, Stockholm, Sweden.
authors.forename: Maria
authors.initials: M
authors.lastname: Soltesova
authors.forename: Jozef
authors.initials: J
authors.lastname: Kowalewski
authors.forename: Göran
authors.initials: G
authors.lastname: Widmalm
mesh_terms: D000465:Algorithms; D002247:Carbon Isotopes / Q000737:chemistry; D017666:Deuterium Oxide / Q000737:chemistry; D004926:Escherichia coli / Q000737:chemistry; D008968:Molecular Conformation; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D019081:O Antigens / Q000032:analysis / Q000737:chemistry*; D012996:Solutions / Q000737:chemistry
chemical_list: D002247:Carbon Isotopes; D019081:O Antigens; D012996:Solutions; D017666:Deuterium Oxide
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