title: Heterotopic bone formation around vessels: pilot study of a new animal model.
abstract: To achieve an easily established, safe, and reproducible animal model for the study of heterotopic bone formation around vessels, a small animal series using New Zealand White rabbits was performed. Three different dosages of recombinant human bone morphogenic protein (rhBMP-2) carried by fibrin matrix were tested. A guided tissue regeneration (GTR) membrane sheet was formed into a tube and allowed to harden; it served both to maintain the space around the vessel bundle and to separate the fibrin matrix with rhBMP-2 from skeletal muscle. Wrapped around the femoral vessel bundle and fixed in place, the tube was filled with the fibrin matrix containing rhBMP-2. The surgical site was closed in layers, and the postoperative healing was uneventful. All animals resumed their full preoperative daily activities 3-4 days after the operation. No adverse events such as wound dehiscence or infection occurred, and all animals could be sacrified at the scheduled date. Micro-computed tomography and histological investigations showed heterotopic bone formation around the vessel bundle in the medium- and high-dosage rhBMP-2 groups. An easy, safe, and reproducible animal model that allows the study of heterotopic bone formation around vessels was successfully established.
authors.affiliation: Discipline of Oral & Maxillofacial Surgery, Faculty of Dentistry, The University of Hong Kong , Hong Kong, China .
authors.forename: Wei-Xin
authors.initials: WX
authors.lastname: Cai
authors.forename: Li-Wu
authors.initials: LW
authors.lastname: Zheng
authors.forename: Franz E
authors.initials: FE
authors.lastname: Weber
authors.forename: Chun-Lei
authors.initials: CL
authors.lastname: Li
authors.forename: Li
authors.initials: L
authors.lastname: Ma
authors.forename: Martin
authors.initials: M
authors.lastname: Ehrbar
authors.forename: Roger A
authors.initials: RA
authors.lastname: Zwahlen
keywords: biomaterials; growth factor; tissue engineering
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