title: The Crosslink formation of 2'-OMe oligonucleotide containing 2-amino-6-vinylpurine protects mRNA from miRNA-mediated silencing.
abstract: Masking the miRNA binding site: Crosslink-forming oligonucleotide (CFO) was used for target gene-specific inhibition of microRNA (miRNA) functions. This method can interfere with specific miRNA-mRNA interactions by recognizing sequences unique to the 3'-UTR that are inherent in each mRNA.
authors.affiliation: Institute of Transformative Bio-molecules, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8602, Japan.
authors.forename: Shinya
authors.initials: S
authors.lastname: Hagihara
authors.forename: Wei-Chen
authors.initials: WC
authors.lastname: Lin
authors.forename: Shuhei
authors.initials: S
authors.lastname: Kusano
authors.forename: Xiao-Guang
authors.initials: XG
authors.lastname: Chao
authors.forename: Tsuneaki
authors.initials: T
authors.lastname: Hori
authors.forename: Shuhei
authors.initials: S
authors.lastname: Imoto
authors.forename: Fumi
authors.initials: F
authors.lastname: Nagatsugi
mesh_terms: D000818:Animals; D001483:Base Sequence; D001665:Binding Sites / Q000187:drug effects; D003432:Cross-Linking Reagents / Q000737:chemistry / Q000494:pharmacology*; D048888:Fireflies; D020868:Gene Silencing / Q000187:drug effects*; D006367:HeLa Cells; D006801:Humans; D008156:Luciferases / Q000737:chemistry / Q000235:genetics; D035683:MicroRNAs; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000737:chemistry / Q000494:pharmacology*; D011687:Purines; D012333:RNA, Messenger / Q000737:chemistry* / Q000378:metabolism; D014753:Vinyl Compounds
chemical_list: C432203:2-amino-6-vinylpurine; D003432:Cross-Linking Reagents; D035683:MicroRNAs; D009838:Oligodeoxyribonucleotides; D011687:Purines; D012333:RNA, Messenger; D014753:Vinyl Compounds; D008156:Luciferases
keywords: crosslinks; gene expression; mRNA; miRNA; oligonucleotides