title: Evaluation of glial fibrillar acidic protein as a marker of hepatic ischemia-reperfusion.
abstract: OBJECTIVE
  To evaluate the expression of Glial Fibrillary Acidic Protein (GFAP) after ischemia-reperfusion injury.
  
  
  METHODS
  twenty four rats were divided into four groups: Control, submitted to anesthesia and liver biopsy; Sham, receiving injection of heparin through the vena cava and hepatic pedicle dissection, with liver biopsy after 24 hours; Ischemia-30, the same as Sham group, plus hepatic pedicle clamping for 30 minutes; and Ischemia-90, the same procedure of Ischemia-30 group, but with clamping period of 90 minutes. After 24 hours of observation, the animals underwent laparotomy and we evaluated their livers macroscopically, microscopically by hematoxylin-eosin (HE) and analyzed the expression of GFAP by Western Blotting.
  
  
  RESULTS
  There was no difference in the gross appearance of the livers between the different experimental groups, all having demonstrated normal morphology. HE analysis showed no significant differences with respect to lobule morphology. On the other hand, in the ischemia groups we observed neutrophilic infiltrates and small areas of necrosis. GFAP expression was similar in all groups, either qualitatively and quantitatively.
  
  
  CONCLUSIONS
  The expression of Glial Fibrillary Acidic Protein did not change in our model of ischemia-reperfusion.
authors.affiliation: Programa de Pós-Graduação em Ciências Cirúrgicas, Departamento de Cirurgia, Faculdade de Medicina, Federal University of Rio de Janeiro - UFRJ, Rio de Janeiro, Brazil.
authors.forename: Giuliano Ancelmo
authors.initials: GA
authors.lastname: Bento
authors.forename: Viviani Reis
authors.initials: VR
authors.lastname: da Cunha
authors.forename: Rodrigo
authors.initials: R
authors.lastname: Martinez
authors.forename: Flávia Carvalho Alcantara
authors.initials: FC
authors.lastname: Gomes
authors.forename: Alberto
authors.initials: A
authors.lastname: Schanaider
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000032:analysis; D005904:Glial Fibrillary Acidic Protein / Q000032:analysis / Q000096:biosynthesis*; D008099:Liver / Q000098:blood supply* / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000378:metabolism*
chemical_list: D015415:Biomarkers; D005904:Glial Fibrillary Acidic Protein