title: Real-time monitoring of circulating tumor cell release during tumor manipulation using in vivo photoacoustic and fluorescent flow cytometry.
abstract: BACKGROUND
  Circulating tumor cells (CTCs) form metastases in distant organs. The purpose of this research was to determine if tumor manipulation could enhance cancer cell release from the primary tumor into the circulatory system.
  
  
  METHODS
  Nude mice were inoculated with melanoma or breast cancer cells. The implanted tumor underwent compression, biopsy, complete resection, or laser treatment. CTCs were monitored in the bloodstream using in vivo photoacoustic and fluorescence flow cytometry.
  
  
  RESULTS
  We discovered that pressure, biopsy, and laser treatment can dramatically increase CTC counts (up to 60-fold), whereas proper tumor resection significantly decrease CTC counts.
  
  
  CONCLUSIONS
  Standard medical procedures could trigger CTC release that may increase the risk of metastases. This finding suggests the guidance of cancer treatment and likely diagnosis by real-time monitoring of CTC dynamics followed by well-timed treatment to reduce CTCs in the blood. In vivo detection of intervention-amplified CTCs could be used for early diagnosis of a small tumor, which is undetectable with conventional methods.
authors.affiliation: Phillips Classic Laser and Nanomedicine Laboratories at the Arkansas Nanomedicine Center, University of Arkansas for Medical Sciences, Little Rock, Arkansas; Department of Otolaryngology - Head and Neck Surgery, Philipps University of Marburg, Marburg, Germany.
authors.forename: Mazen A
authors.initials: MA
authors.lastname: Juratli
authors.forename: Mustafa
authors.initials: M
authors.lastname: Sarimollaoglu
authors.forename: Eric R
authors.initials: ER
authors.lastname: Siegel
authors.forename: Dmitry A
authors.initials: DA
authors.lastname: Nedosekin
authors.forename: Ekaterina I
authors.initials: EI
authors.lastname: Galanzha
authors.forename: James Y
authors.initials: JY
authors.lastname: Suen
authors.forename: Vladimir P
authors.initials: VP
authors.lastname: Zharov
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D005434:Flow Cytometry / Q000379:methods*; D051379:Mice; D008819:Mice, Nude; D009360:Neoplastic Cells, Circulating
keywords: breast cancer; circulating tumor cells; in vivo flow cytometry; melanoma; photoacoustic and fluorescence methods
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