title: Mathematical classification of tight junction protein images.
abstract: We present the rationale for the development of mathematical features used for classification of images stained for selected tight junction proteins. The project examined localization of zonula occludens-1, claudin-1 and F-actin in a model epithelium, Madin-Darby canine kidney II cells. Cytochalasin D exposure was used to perturb junctional localization by actin cytoskeleton disruption. Mathematical features were extracted from images to reliably reveal characteristic information of the pattern of protein localization. Features, such as neighbourhood standard deviation, gradient of pixel intensity measurement and conditional probability, provided meaningful information to classify complex image sets. The newly developed mathematical features were used as input to train a neural network that provided a robust method of individual image classification. The ability for researchers to make determinations concerning image classification while minimizing human bias is an important advancement for the field of tight junction cellular biology.
authors.affiliation: Trinity University, San Antonio, Texas, U.S.A.
authors.forename: K H
authors.initials: KH
authors.lastname: Ogawa
authors.forename: C M
authors.initials: CM
authors.lastname: Troyer
authors.forename: R G
authors.initials: RG
authors.lastname: Doss
authors.forename: F
authors.initials: F
authors.lastname: Aminian
authors.forename: E C
authors.initials: EC
authors.lastname: Balreira
authors.forename: J M
authors.initials: JM
authors.lastname: King
mesh_terms: D000199:Actins / Q000378:metabolism*; D000818:Animals; D002460:Cell Line; D062445:Claudin-1 / Q000378:metabolism*; D015638:Cytochalasin D / Q000378:metabolism; D003599:Cytoskeleton / Q000378:metabolism; D004285:Dogs; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism; D005455:Fluorescent Antibody Technique / Q000379:methods; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D061985:Madin Darby Canine Kidney Cells; D008432:Mathematical Computing; D008962:Models, Theoretical; D019108:Tight Junctions / Q000378:metabolism*; D062826:Zonula Occludens-1 Protein / Q000378:metabolism*
chemical_list: D000199:Actins; D062445:Claudin-1; D062826:Zonula Occludens-1 Protein; D015638:Cytochalasin D
keywords: Claudin; ZO-1modeling; cytochalasin D; cytoskeleton; edge detection; image classification; modeling; neural networks; tight junction