title: The interplay between protein stability and dynamics in conformational diseases: the case of hPGK1 deficiency.
abstract: Conformational diseases often show defective protein folding efficiency in vivo upon mutation, affecting protein properties such as thermodynamic stability and folding/unfolding/misfolding kinetics as well as the interactions of the protein with the protein homeostasis network. Human phosphoglycerate kinase 1 (hPGK1) deficiency is a rare inherited disease caused by mutations in hPGK1 that lead to loss-of-function. This disease offers an excellent opportunity to explore the complex relationships between protein stability and dynamics because of the different unfolding mechanisms displayed towards chemical and thermal denaturation. This work explores these relationships using two thermostable mutants (p.E252A and p.T378P) causing hPGK1 deficiency and WT hPGK1 using proteolysis and chemical denaturation. p.T378P is degraded ~30-fold faster at low protease concentrations (here, the proteolysis step is rate-limiting) and ~3-fold faster at high protease concentrations (where unfolding kinetics is rate-limiting) than WT and p.E252A, indicating that p.T378P is thermodynamically and kinetically destabilized. Urea denaturation studies support the decrease in thermodynamic stability and folding cooperativity for p.T378P, as well as changes in folding/unfolding kinetics. The present study reveals changes in the folding landscape of hPGK1 upon mutation that may affect protein folding efficiency and stability in vivo, also suggesting that native state stabilizers and protein homeostasis modulators may help to correct folding defects in hPGK1 deficiency. Moreover, detailed kinetic proteolysis studies are shown to be powerful and simple tools to provide deep insight into mutational effects on protein folding and stability in conformational diseases.
authors.affiliation: Department of Physical Chemistry, Faculty of Sciences, University of Granada, Granada, Spain. Electronic address: angelpey@ugr.es.
authors.forename: Angel L
authors.initials: AL
authors.lastname: Pey
mesh_terms: D019943:Amino Acid Substitution; D004795:Enzyme Stability; D040181:Genetic Diseases, X-Linked / Q000201:enzymology* / Q000235:genetics; D006801:Humans; D008661:Metabolism, Inborn Errors / Q000201:enzymology* / Q000235:genetics; D020125:Mutation, Missense; D010735:Phosphoglycerate Kinase / Q000737:chemistry / Q000172:deficiency* / Q000235:genetics / Q000378:metabolism; D011489:Protein Denaturation; D059748:Proteolysis; D014508:Urea / Q000737:chemistry
chemical_list: D014508:Urea; C577282:PGK1 protein, human; D010735:Phosphoglycerate Kinase
keywords: CD; Conformational disease; DSP; Mutations; PGK; Protein stability; Proteolysis; T(m); WT; circular dichroism; denaturation temperature; differential scanning proteolysis; phosphoglycerate kinase; wild-type