title: Increasing protein production by directed vector backbone evolution.
abstract: Recombinant protein production in prokaryotic and eukaryotic organisms was a key enabling technology for the rapid development of industrial and molecular biotechnology. However, despite all progress the improvement of protein production is an ongoing challenge and of high importance for cost-effective enzyme production. With the epMEGAWHOP mutagenesis protocol for vector backbone optimization we report a novel directed evolution based approach to increase protein production levels by randomly introducing mutations in the vector backbone. In the current study we validate the epMEGAWHOP mutagenesis protocol for three different expression systems. The latter demonstrated the general applicability of the epMEGAWHOP method. Cellulase and lipase production was doubled in one round of directed evolution by random mutagenesis of pET28a(+) and pET22b(+) vector backbones. Protease production using the vector pHY300PLK was increased ~4-times with an average of ~1.25 mutations per kb vector backbone. The epMEGAWHOP does not require any rational understanding of the expression machinery and can generally be applied to enzymes, expression vectors and related hosts. epMEGAWHOP is therefore from our point of view a robust, rapid and straight forward alternative for increasing protein production in general and for biotechnological applications.
authors.affiliation: Lehrstuhl für Biotechnologie, RWTH Aachen University, Worringerweg 1, Aachen 52074, Germany. u.schwaneberg@biotec.rwth-aachen.de.
authors.forename: Felix
authors.initials: F
authors.lastname: Jakob
authors.forename: Christian
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authors.lastname: Lehmann
authors.forename: Ronny
authors.initials: R
authors.lastname: Martinez
authors.forename: Ulrich
authors.initials: U
authors.lastname: Schwaneberg
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