title: DBZ (DISC1-binding zinc finger protein)-deficient mice display abnormalities in basket cells in the somatosensory cortices.
abstract: Disrupted-in-schizophrenia 1 (DISC1)-binding zinc finger protein (DBZ) is a DISC1-interacting molecule and the interaction between DBZ and DISC1 is involved in neurite outgrowth in vitro. DBZ is highly expressed in brain, especially in the cortex. However, the physiological roles of DBZ in vivo have not been clarified. Here, we show that development of basket cells, a morphologically defined class of parvalbumin (PV)-containing interneurons, is disturbed in DBZ knockout (KO) mice. DBZ mRNA was highly expressed in the ventral area of the subventricular zone of the medial ganglionic eminence, where PV-containing cortical interneurons were generated, at embryonic 14.5 days (E14.5). Although the expression level for PV and the number of PV-containing interneurons were not altered in the cortices of DBZ KO mice, basket cells were less branched and had shorter processes in the somatosensory cortices of DBZ KO mice compared with those in the cortices of WT mice. Furthermore, in the somatosensory cortices of DBZ KO mice, the level of mRNAs for the gamma-aminobutyric acid-synthesizing enzymes GAD67 was decreased. These findings show that DBZ is involved in the morphogenesis of basket cells.
authors.affiliation: Department of Anatomy and Neuroscience, Graduate School of Medicine, Osaka University, 2-2 Yamadaoka, Suita, Osaka 565-0871, Japan.
authors.forename: Yoshihisa
authors.initials: Y
authors.lastname: Koyama
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Hattori
authors.forename: Shoko
authors.initials: S
authors.lastname: Shimizu
authors.forename: Manabu
authors.initials: M
authors.lastname: Taniguchi
authors.forename: Kohei
authors.initials: K
authors.lastname: Yamada
authors.forename: Hironori
authors.initials: H
authors.lastname: Takamura
authors.forename: Natsuko
authors.initials: N
authors.lastname: Kumamoto
authors.forename: Shinsuke
authors.initials: S
authors.lastname: Matsuzaki
authors.forename: Akira
authors.initials: A
authors.lastname: Ito
authors.forename: Taiichi
authors.initials: T
authors.lastname: Katayama
authors.forename: Masaya
authors.initials: M
authors.lastname: Tohyama
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000378:metabolism*; D005968:Glutamate Decarboxylase / Q000096:biosynthesis; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D007395:Interneurons / Q000378:metabolism / Q000473:pathology*; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D018613:Microscopy, Confocal; D009419:Nerve Tissue Proteins / Q000172:deficiency / Q000378:metabolism*; D055495:Neurogenesis / Q000502:physiology*; D013003:Somatosensory Cortex / Q000473:pathology*
chemical_list: D002352:Carrier Proteins; C498794:Disc1 protein, mouse; D009419:Nerve Tissue Proteins; C017800:zinc-binding protein; D005968:Glutamate Decarboxylase; C402565:glutamate decarboxylase 1
keywords: Basket cell; Branching; CGE; CR; DBZ; DISC1; DISC1-binding zinc finger protein; ES; GABA; GAD; GAD67; Interneuron; KO; Knockout mouse; LGE; MGE; PV; SOM; SVZ; WT; cDNA; calretinin; caudal ganglionic eminence; complementary DNA; disrupted-in-schizophrenia 1; embryonic stem; gamma-aminobutyric acid; glutamic acid decarboxylase isoform 67; knockout; lateral ganglionic eminence; mRNA; medial ganglionic eminence; messenger RNA; parvalbumin; somatostatin; subventricular zone; wild type