title: Preparation of chitin nanogels containing nickel nanoparticles.
abstract: In this work, we developed 120-150 nm sized nickel nanoparticles loaded chitin nanogels (Ni-Chitin NGs) by regeneration chemistry approach to investigate and determine its cytocompatibility and antibacterial activity against Staphylococcus aureus. The nickel nanoparticles were prepared by hydrothermal method. The prepared Ni-Chitin NGs were well characterized by SEM, FTIR, TG/DTA/DTG and XRD and the in vitro cytocompatibility was tested on A549 and L929 cells which showed that they are completely non-toxic. Ni-Chitin NGs showed better toxicity to the bacterial strains when compared to previous study with other nanoparticles using serial dilution method. The rhodamine labeled-Ni-Chitin NGs showed cellular localization on both L929 and A549 cells without perturbing their cellular constituents. These studies showed that the Ni-Chitin NGs could be used for various applications in biomedical filed.
authors.affiliation: Amrita Centre for Nanosciences and Molecular Medicine, Amrita Institute of Medical Sciences and Research Centre, Amrita Vishwa Vidyapeetham University, Cochin 682041, India.
authors.forename: N Ashwin
authors.initials: NA
authors.lastname: Kumar
authors.forename: N Sanoj
authors.initials: NS
authors.lastname: Rejinold
authors.forename: P
authors.initials: P
authors.lastname: Anjali
authors.forename: Avinash
authors.initials: A
authors.lastname: Balakrishnan
authors.forename: Raja
authors.initials: R
authors.lastname: Biswas
authors.forename: R
authors.initials: R
authors.lastname: Jayakumar
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D045744:Cell Line, Tumor; D002686:Chitin / Q000138:chemical synthesis* / Q000494:pharmacology; D004055:Differential Thermal Analysis; D006801:Humans; D006863:Hydrogen-Ion Concentration / Q000187:drug effects; D051379:Mice; D008826:Microbial Sensitivity Tests; D000080385:Nanogels; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D009532:Nickel / Q000737:chemistry* / Q000494:pharmacology; D010316:Particle Size; D011092:Polyethylene Glycols / Q000138:chemical synthesis* / Q000494:pharmacology; D011094:Polyethyleneimine / Q000138:chemical synthesis* / Q000494:pharmacology; D020112:Rhodamine 123 / Q000378:metabolism; D017550:Spectroscopy, Fourier Transform Infrared; D013211:Staphylococcus aureus / Q000187:drug effects; D013818:Thermogravimetry; D014961:X-Ray Diffraction
chemical_list: D000900:Anti-Bacterial Agents; D000080385:Nanogels; C413692:polyethylene glycol polyethyleneimine nanogel; D002686:Chitin; D020112:Rhodamine 123; D011092:Polyethylene Glycols; D009532:Nickel; D011094:Polyethyleneimine
keywords: Antibacterial activity; Biomedical applications; Chitin nanogels; Cytocompatibility; Nickel nanoparticles