title: Analysis of pomegranate juice components in rat corpora cavernosal relaxation.
abstract: This study evaluated the action of pomegranate juice (PJ) and its five principal phenolic constituents on rat corpus cavernosum smooth muscle (CCSM). Isometric tension studies were performed after precontraction with phenylephrine in CCSM from rats. Relaxant responses to PJ and its constituents ellagic acid (EA), chlorogenic acid, caffeic acid, cumaric acid and rutin were investigated. PJ and EA caused CCSM relaxations (94.1 ± 3.7 and 51.3 ± 9.9%), while others induced limited relaxant responses. EA response was not inhibited by L-N(G)-nitroarginine methyl ester (100 μM) and 1H-[1,2,4]-oxadiazolo[4,3-a]quinoxalin-1-one (1 μM). Tetraethylammonium (100 μM) and apamin (10 μM) and nifedipine (10 μM) inhibited EA-induced relaxations at 10(-3) M by 84%, 82% and 78%, respectively. Glibenclamide (10 μM) inhibited EA response (97%, 100 μM). PJ-induced relaxation was not altered by several inhibitors. EA was estimated to be responsible for 13.3% of relaxation caused by PJ. Our study demonstrated that PJ and EA-induced marked relaxations in CCSM. The opening of Ca(2+)-activated K+ channels and the inhibition of Ca(2+)-channels regulate the relaxation by EA, but not PJ. EA has a minor contribution to the marked relaxation obtained by PJ, suggesting the presence of other PJ constituents, which induce nitric oxide-independent corporal relaxation. Further studies are needed to examine the potential of PJ in combination with a PDE5 inhibitor in ED.
authors.affiliation: Department of Urology, Ankara Numune Education and Research Hospital, Ankara, Turkey.
authors.forename: C V
authors.initials: CV
authors.lastname: Oztekin
authors.affiliation: Faculty of Pharmacy, Department of Pharmacology, Ankara University, Ankara, Turkey.
authors.forename: S
authors.initials: S
authors.lastname: Gur
authors.affiliation: Faculty of Pharmacy, Department of Pharmacology, Ankara University, Ankara, Turkey.
authors.forename: N A
authors.initials: NA
authors.lastname: Abdulkadir
authors.affiliation: Department of Pharmacognosy, Ankara University, Ankara, Turkey.
authors.forename: M
authors.initials: M
authors.lastname: Kartal
authors.affiliation: Department of Urology, Ankara Numune Education and Research Hospital, Ankara, Turkey.
authors.forename: M
authors.initials: M
authors.lastname: Karabakan
authors.affiliation: Department of Urology, Ankara Numune Education and Research Hospital, Ankara, Turkey.
authors.forename: A O
authors.initials: AO
authors.lastname: Akdemir
authors.affiliation: Department of Urology, Ankara Numune Education and Research Hospital, Ankara, Turkey.
authors.forename: C S
authors.initials: CS
authors.lastname: Gökkaya
authors.affiliation: Department of Urology, Ankara Numune Education and Research Hospital, Ankara, Turkey.
authors.forename: M
authors.initials: M
authors.lastname: Cetinkaya
mesh_terms: D000818:Animals; D001030:Apamin; D005576:Colforsin; D004610:Ellagic Acid / Q000032:analysis / Q000494:pharmacology; D005905:Glyburide; D066298:In Vitro Techniques; D029561:Lythraceae; D008297:Male; D009126:Muscle Relaxation / Q000187:drug effects*; D009130:Muscle, Smooth / Q000187:drug effects*; D019331:NG-Nitroarginine Methyl Ester; D009543:Nifedipine; D010069:Oxadiazoles; D010413:Penis / Q000187:drug effects*; D010879:Piperazines; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D011687:Purines; D011810:Quinoxalines; D051381:Rats; D000068677:Sildenafil Citrate; D013450:Sulfones; D019789:Tetraethylammonium
chemical_list: C095284:1H-(1,2,4)oxadiazolo(4,3-a)quinoxalin-1-one; D010069:Oxadiazoles; D010879:Piperazines; D010936:Plant Extracts; D011687:Purines; D011810:Quinoxalines; D013450:Sulfones; D004610:Ellagic Acid; D005576:Colforsin; D001030:Apamin; D019789:Tetraethylammonium; D000068677:Sildenafil Citrate; D009543:Nifedipine; D005905:Glyburide; D019331:NG-Nitroarginine Methyl Ester
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