title: Burdock fructooligosaccharide induces stomatal closure in Pisum sativum.
abstract: Burdock fructooligosaccharide (BFO) isolated from the root tissue of Arctium lappa is a reserve carbohydrate that can induce resistance against a number of plant diseases. Stomatal closure is a part of plant innate immune response to restrict bacterial invasion. In this study, the effects of BFO on stomata movement in Pisum sativum and the possible mechanisms were studied with abscisic acid (ABA) as a positive control. The results showed that BFO could induce stomatal closure accompanied by ROS and NO production, as is the case with ABA. BFO-induced stomatal closure was inhibited by pre-treatment with L-NAME (N(G)-nitro-L-arginine methyl ester, hydrochloride; nitric oxide synthase inhibitor) and catalase (hydrogen peroxide scavenger). Exogenous catalase completely restricted BFO-induced production of ROS and NO in guard cells. In contrast, L-NAME prevented the rise in NO levels but only partially restricted the ROS production. These results indicate that BFO-induced stomatal closure is mediated by ROS and ROS-dependent NO production.
authors.affiliation: School of Life Sciences and National Glycoengineering Research Center, Shandong University, Jinan 250100, China.
authors.forename: Yanling
authors.initials: Y
authors.lastname: Guo
authors.forename: Moran
authors.initials: M
authors.lastname: Guo
authors.forename: Wenlu
authors.initials: W
authors.lastname: Zhao
authors.forename: Kaoshan
authors.initials: K
authors.lastname: Chen
authors.forename: Pengying
authors.initials: P
authors.lastname: Zhang
mesh_terms: D000040:Abscisic Acid / Q000494:pharmacology; D031170:Arctium / Q000737:chemistry*; D009569:Nitric Oxide / Q000378:metabolism; D009844:Oligosaccharides / Q000494:pharmacology*; D018532:Pisum sativum / Q000166:cytology / Q000187:drug effects* / Q000502:physiology*; D054046:Plant Stomata / Q000166:cytology / Q000187:drug effects* / Q000502:physiology*; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D009844:Oligosaccharides; D017382:Reactive Oxygen Species; C116580:fructooligosaccharide; D009569:Nitric Oxide; D000040:Abscisic Acid
keywords: Burdock fructooligosaccharide; Nitric oxide; Pisum sativum; Reactive oxygen species; Stomata