title: Evaluation of the association of NKG2C copy number variations with susceptibility to human papillomavirus-induced cervical lesions.
abstract: Squamous intraepithelial lesions (SIL) and cervical cancer are primary due to suboptimal host-dependent immune response against human papillomavirus (HPV). Natural killer cells (NK) are innate-immune response components against virus and tumors. We studied whether the null allele of NKG2C NK cell receptor could be associated with low-grade (LSIL) to high-grade SIL (HSIL) transition or likelihood of HPV infection. Eight-hundred and sixty-seven subjects (263 LSIL, 309 HSIL and 295 controls) were genotyped for NKG2C using a novel multiplex PCR protocol. HPV genotype information was obtained from the cases. NKG2C genotype distributions in LSIL were WT/WT: 69.2%, WT/null: 26.2% and null/null: 4.6%; whereas in HSIL were WT/WT: 65.4%, WT/null: 28.5% and null/null: 6.1% and no statistical differences were observed (LSIL vs. HSIL p=0.541; LSIL vs. controls p=0.230; HSIL vs. controls p=0.624) nor when restricting to HPV positive, HR-HPV nor co-infection. This study demonstrates that NKG2Cnull does not seem to constitute a risk factor for HPV-induced cervical lesions.
authors.affiliation: Molecular Pathology and Pharmacogenetic Group, Clinical Analysis Department, Santa Lucía General University Hospital (HGUSL), 30202 Cartagena, Spain. Electronic address: jrvilchez2000@gmail.com.
authors.forename: José R
authors.initials: JR
authors.lastname: Vilchez
authors.forename: Daniel
authors.initials: D
authors.lastname: Torres-Moreno
authors.forename: María M
authors.initials: MM
authors.lastname: Martínez-Senac
authors.forename: Javier
authors.initials: J
authors.lastname: Trujillo-Santos
authors.forename: Pablo
authors.initials: P
authors.lastname: Conesa-Zamora
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D056915:DNA Copy Number Variations; D005260:Female; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D055654:NK Cell Lectin-Like Receptor Subfamily C / Q000235:genetics*; D027383:Papillomaviridae / Q000235:genetics; D030361:Papillomavirus Infections / Q000150:complications*; D012307:Risk Factors; D055815:Young Adult; D002578:Uterine Cervical Dysplasia / Q000235:genetics* / Q000821:virology*
chemical_list: C527059:KLRC2 protein, human; D055654:NK Cell Lectin-Like Receptor Subfamily C
keywords: CC; HCMV; HLA; HPV; HR-HPV; HSIL; KIR; LR-HPV; LSIL; MHC; NK; PCR; SIL; WT; cervical carcinoma; high-grade squamous intraepithelial lesions; high-risk HPV; human cytomegalovirus; human leukocyte antigen; human papillomavirus; killer immunoglobulin-like receptor; low-grade squamous intraepithelial lesions; low-risk HPV; major histocompatibility complex; natural killer; polymerase chain reaction; squamous intraepithelial lesions; wild-type