title: Wound healing potential of formulated extract from hibiscus sabdariffa calyx.
abstract: Wound healing agents support the natural healing process, reduce trauma and likelihood of secondary infections and hasten wound closure. The wound healing activities of water in oil cream of the methanol extract of Hibiscus sabdariffa L. (Malvaceae) was evaluated in rats with superficial skin excision wounds. Antibacterial activities against Pseudomonas aeroginosa, Staphylococcus aureus and Echerichia coli were determined. The total flavonoid content, antioxidant properties and thin layer chromatographic fingerprints of the extract were also evaluated. The extract demonstrated antioxidant properties with a total flavonoid content of 12.30±0.09 mg/g. Six reproducible spots were obtained using methanol:water (95:5) as the mobile phase. The extract showed no antimicrobial activity on the selected microorganisms, which are known to infect and retard wound healing. Creams containing H. sabdariffa extract showed significant (P<0.05) and concentration dependent wound healing activities. There was also evidence of synergism with creams containing a combination of gentamicin and H. sabdariffa extract. This study, thus, provides evidence of the wound healing potentials of the formulated extract of the calyces of H. sabdariffa and synergism when co-formulated with gentamicin.
authors.affiliation: Department of Pharmaceutical Technology and Raw Material Development, National Institute for Pharmaceutical Research and Development, Abuja, Nigeria.
authors.forename: P F
authors.initials: PF
authors.lastname: Builders
authors.forename: B
authors.initials: B
authors.lastname: Kabele-Toge
authors.forename: M
authors.initials: M
authors.lastname: Builders
authors.forename: B A
authors.initials: BA
authors.lastname: Chindo
authors.forename: Patricia A
authors.initials: PA
authors.lastname: Anwunobi
authors.forename: Yetunde C
authors.initials: YC
authors.lastname: Isimi
keywords: Antimicrobial activity; Hibiscus sabdariffa calyx; antioxidant activity; water in oil cream; wound healing
references.citation: Phytother Res. 2006 Nov;20(11):941-4
references.pmid: 16909445
references.citation: J Ethnopharmacol. 1997 Apr;56(2):139-44
references.pmid: 9174975
references.citation: J Ethnopharmacol. 2004 Dec;95(2-3):253-8
references.pmid: 15507345
references.citation: Plant Foods Hum Nutr. 1998;52(1):17-30
references.pmid: 9839831
references.citation: Front Biosci. 2004 Jan 01;9:283-9
references.pmid: 14766366
references.citation: Phytother Res. 2010 Oct;24(10):1437-41
references.pmid: 20878690
references.citation: Afr J Tradit Complement Altern Med. 2009 Mar 07;6(2):168-74
references.pmid: 20209009
references.citation: Ann Pharmacother. 1997 May;31(5):559-63
references.pmid: 9161648
references.citation: Plant Physiol. 2007 Dec;145(4):1323-35
references.pmid: 17921343
references.citation: Aesthetic Plast Surg. 2003 Sep-Oct;27(5):411-7
references.pmid: 14648065
references.citation: J Pharm Sci. 1995 Apr;84(4):466-72
references.pmid: 7629738
references.citation: Nat Prod Res. 2008 Mar 10;22(4):296-304
references.pmid: 18322845
references.citation: J Ethnopharmacol. 1998 Jan;59(3):179-86
references.pmid: 9507902
references.citation: Home Healthc Nurse. 2004 Sep;22(9):586-90
references.pmid: 15359168
references.citation: Am J Surg. 1994 Jan;167(1A):42S-44S; discussion 44S-45S
references.pmid: 8109684
references.citation: Am Fam Physician. 1998 Jun;57(11):2731-40
references.pmid: 9636336
references.citation: Evid Based Complement Alternat Med. 2008 Mar;5(1):95-101
references.pmid: 18317555
references.citation: Food Chem Toxicol. 2000 May;38(5):411-6
references.pmid: 10762726
references.citation: Pharmacognosy Res. 2010 Mar;2(2):63-8
references.pmid: 21808542
references.citation: Clin Microbiol Rev. 2001 Apr;14(2):244-69
references.pmid: 11292638
references.citation: Arch Int Pharmacodyn Ther. 1972 Mar;196(1):117-26
references.pmid: 5059357
references.citation: AAPS PharmSciTech. 2005 Oct 31;6(4):E565-72
references.pmid: 16408858
references.citation: J Hosp Infect. 1998 Nov;40(3):175-8
references.pmid: 9830588