title: Exploring the glycosylation of serum CA125.
abstract: Ovarian cancer is the most lethal gynaecologic cancer affecting women. The most widely used biomarker for ovarian cancer, CA125, lacks sensitivity and specificity. Here, we explored differences in glycosylation of CA125 between serum from patients with ovarian cancer and healthy controls. We found differences between CA125 N-glycans from patient sera compared to controls. These include increases in core-fucosylated bi-antennary monosialylated glycans, as well as decreases in mostly bisecting bi-antennary and non-fucosylated glycans in patients compared to controls. Measurement of the glycosylated state of CA125 may therefore provide a more specific biomarker for patients with ovarian cancer.
authors.affiliation: NIBRT GlycoScience Group, National Institute for Bioprocessing Research and Training, Mount Merrion, Blackrock, Dublin 4, Ireland. radka.fahey@nibrt.ie
authors.forename: Radka
authors.initials: R
authors.lastname: Saldova
authors.forename: Weston B
authors.initials: WB
authors.lastname: Struwe
authors.forename: Kieran
authors.initials: K
authors.lastname: Wynne
authors.forename: Giuliano
authors.initials: G
authors.lastname: Elia
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Duffy
authors.forename: Pauline M
authors.initials: PM
authors.lastname: Rudd
mesh_terms: D015415:Biomarkers / Q000097:blood; D018394:CA-125 Antigen / Q000097:blood* / Q000378:metabolism; D002240:Carbohydrate Sequence; D002851:Chromatography, High Pressure Liquid; D005260:Female; D005643:Fucose / Q000737:chemistry; D006031:Glycosylation; D006801:Humans; D008969:Molecular Sequence Data; D010051:Ovarian Neoplasms / Q000097:blood / Q000473:pathology; D011134:Polysaccharides / Q000032:analysis; D053719:Tandem Mass Spectrometry
chemical_list: D015415:Biomarkers; D018394:CA-125 Antigen; D011134:Polysaccharides; D005643:Fucose
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