title: FimH adhesin from host unrestricted Salmonella Enteritidis binds to different glycoprotein ligands expressed by enterocytes from sheep, pig and cattle than FimH adhesins from host restricted Salmonella Abortus-ovis, Salmonella Choleraesuis and Salmonella Dublin.
abstract: Adhesion to gut tissues and colonization of the alimentary tract, two important stages in the pathogenesis of Salmonella, are mediated by FimH adhesin of type 1 fimbriae. It was suggested that minor differences in the structure of FimH are most likely associated with differences in adhesion specificities, and may determine the tropism of various Salmonella serovars to different species and tissues. We investigated this hypothesis by comparing the binding properties of FimH proteins from three Salmonella enterica serovars with limited (Choleraesuis, Dublin) or restricted (Abortusovis) host ranges to FimH from broad host range S. Enteritidis and mannose inactive FimH from S. Gallinarum. Although all active variants of FimH protein were able to bind mannose-rich glycoproteins (RNase B, HRP and Man-BSA) with comparable affinity measured by surface plasmon resonance, there were significant differences in the binding profiles of the FimH proteins from host restricted serovars and host unrestricted serovar Enteritidis, to glycoproteins from enterocyte cell lines established in vitro and derived from sheep, pig and cattle. When low-binding FimH adhesin from S. Enteritidis was subjected to Western blot analysis, it bound to surface membrane protein of about 130 kDa, and high-binding FimH adhesins from S. Abortusovis, S. Choleraesuis and S. Dublin bound to surface membrane protein of about 55 kDa present in each cell line. Differential binding of FimH proteins from host-restricted and broad-host-range Salmonella to intestinal receptors was confirmed using mutant FimH adhesins obtained by site-directed mutagenesis. It was found that the low-binding variant of FimH from S. Choleraesuis with mutation Leu57Pro lost the ability to bind protein band of 55 kDa, but instead interacted with glycoprotein of about 130 kDa. On the other hand, the high-binding variant of FimH adhesin from S. Enteritids with mutation Asn101Ser did not bind to its receptor of 130 kDa, but instead it interacted with glycoprotein ligand of 55 kDa. These results suggest that FimH adhesins of type 1 fimbriae are one of the factors responsible for different host-specificities of these Salmonella serovars.
authors.affiliation: Department of Biochemistry, Pharmacology and Toxicology, Wrocław University of Environmental and Life Sciences, 50-375 Wrocław, Poland. krzysztof.grzymajlo@up.wroc.pl
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Grzymajło
authors.forename: Maciej
authors.initials: M
authors.lastname: Ugorski
authors.forename: Rafał
authors.initials: R
authors.lastname: Kolenda
authors.forename: Anna
authors.initials: A
authors.lastname: Kędzierska
authors.forename: Marta
authors.initials: M
authors.lastname: Kuźmińska-Bajor
authors.forename: Alina
authors.initials: A
authors.lastname: Wieliczko
mesh_terms: D018829:Adhesins, Bacterial / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D015153:Blotting, Western; D002417:Cattle; D002418:Cattle Diseases / Q000378:metabolism* / Q000382:microbiology; D002460:Cell Line; D020895:Enterocytes / Q000378:metabolism* / Q000382:microbiology; D010861:Fimbriae, Bacterial / Q000235:genetics / Q000378:metabolism; D006023:Glycoproteins / Q000235:genetics / Q000378:metabolism*; D058507:Host Specificity; D007413:Intestinal Mucosa / Q000378:metabolism; D008024:Ligands; D016297:Mutagenesis, Site-Directed; D011485:Protein Binding; D012475:Salmonella / Q000235:genetics / Q000378:metabolism*; D012477:Salmonella enteritidis / Q000235:genetics / Q000378:metabolism*; D012756:Sheep; D012757:Sheep Diseases / Q000378:metabolism* / Q000382:microbiology; D034421:Sus scrofa; D013552:Swine; D013553:Swine Diseases / Q000378:metabolism* / Q000382:microbiology
chemical_list: D018829:Adhesins, Bacterial; D006023:Glycoproteins; D008024:Ligands
keywords: FimH adhesin; Host specificity; Salmonella enterica; Type 1 fimbriae