title: A characterization of four B16 murine melanoma cell sublines molecular fingerprint and proliferation behavior.
abstract: BACKGROUND
  One of the most popular and versatile model of murine melanoma is by inoculating B16 cells in the syngeneic C57BL6J mouse strain. A characterization of different B16 modified cell sub-lines will be of real practical interest. For this aim, modern analytical tools like surface enhanced Raman spectroscopy/scattering (SERS) and MTT were employed to characterize both chemical composition and proliferation behavior of the selected cells.
  
  
  METHODS
  High quality SERS signal was recorded from each of the four types of B16 cell sub-lines: B164A5, B16GMCSF, B16FLT3, B16F10, in order to observe the differences between a parent cell line (B164A5) and other derived B16 cell sub-lines. Cells were incubated with silver nanoparticles of 50-100 nm diameter and the nanoparticles uptake inside the cells cytoplasm was proved by transmission electron microscopy (TEM) investigations. In order to characterize proliferation, growth curves of the four B16 cell lines, using different cell numbers and FCS concentration were obtained employing the MTT proliferation assay. For correlations doubling time were calculated.
  
  
  RESULTS
  SERS bands allowed the identification inside the cells of the main bio-molecular components such as: proteins, nucleic acids, and lipids. An "on and off" SERS effect was constantly present, which may be explained in terms of the employed laser power, as well as the possible different orientations of the adsorbed species in the cells in respect to the Ag nanoparticles. MTT results showed that among the four tested cell sub-lines B16 F10 is the most proliferative and B164A5 has the lower growth capacity. Regarding B16FLT3 cells and B16GMCSF cells, they present proliferation ability in between with slight slower potency for B16GMCSF cells.
  
  
  CONCLUSIONS
  Molecular fingerprint and proliferation behavior of four B16 melanoma cell sub-lines were elucidated by associating SERS investigations with MTT proliferation assay.
authors.affiliation: Faculty of Pharmacy, University of Medicine and Pharmacy "Victor Babes", EftimieMurgu Square, No. 2, 300041 Timişoara, România.
authors.forename: Corina
authors.initials: C
authors.lastname: Danciu
authors.affiliation: Biomedical Physics, Biomedical, Theoretical Physics, and Molecular Spectroscopy Department, Faculty of Physics, Babes-Bolyai University, Kogalniceanu 1, RO 400084 Cluj-Napoca, România.
authors.forename: Alexandra
authors.initials: A
authors.lastname: Falamas
authors.affiliation: Faculty of Pharmacy, University of Medicine and Pharmacy "Victor Babes", EftimieMurgu Square, No. 2, 300041 Timişoara, România.
authors.forename: Cristina
authors.initials: C
authors.lastname: Dehelean
authors.affiliation: Faculty of Pharmacy, University of Medicine and Pharmacy "Victor Babes", EftimieMurgu Square, No. 2, 300041 Timişoara, România.
authors.forename: Codruta
authors.initials: C
authors.lastname: Soica
authors.affiliation: Pharmazentrum Frankfurt/Center for Drug Research, Development and Safety, Clinic of J.W. Goethe University, Theodor-Stern-Kai 7, 60590 Frankfurt, Germany.
authors.forename: Heinfried
authors.initials: H
authors.lastname: Radeke
authors.affiliation: Electron Microscopy Center Faculty of Biology & Geology "Babes-Bolyai", University of Cluj-Napoca, 5-7 Clinicilor Street, 400006 Cluj-Napoca, Romania.
authors.forename: Lucian
authors.initials: L
authors.lastname: Barbu-Tudoran
authors.affiliation: Department of Physiology and Immunology, University of Medicine and Pharmacy "Victor Babes", Eftimie Murgu Square, No. 2, 300041 Timisoara, Romania.
authors.forename: Florina
authors.initials: F
authors.lastname: Bojin
authors.affiliation: Biomedical Physics, Biomedical, Theoretical Physics, and Molecular Spectroscopy Department, Faculty of Physics, Babes-Bolyai University, Kogalniceanu 1, RO 400084 Cluj-Napoca, România.
authors.forename: Simona Cîntă
authors.initials: SC
authors.lastname: Pînzaru
authors.affiliation: Department of Clinical Laboratory and Sanitary Chemistry, "Vasile Goldis" University, 1 Feleacului Str., Arad 310396 Romania.
authors.forename: Melania F
authors.initials: MF
authors.lastname: Munteanu
keywords: B16 cells; Doubling time; MTT; SERS; TEM
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