title: A new strategy for early diagnosis of type 2 diabetes by standard-free, label-free LC-MS/MS quantification of glycated peptides.
abstract: The early diagnosis of diabetes, one of the top three chronic incurable diseases, is becoming increasingly important. Here, we investigated the applicability of an (18)O-labeling technique for the development of a standard-free, label-free liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the early diagnosis of type 2 diabetes mellitus (T2DM). Rather than attempting to identify quantitative differences in proteins as biomarkers, glycation of the highest abundance protein in human plasma, human serum albumin (HSA), was monitored through quantitative analysis of HSA characteristic peptides using the (18)O-labeling technique. Eight glucose-sensitive peptides and one glucose-insensitive peptide were discovered. The glucose-insensitive peptide served as the internal standard, and a standard-free, label-free LC-MS/MS method was developed. This method was then used to select putative biomarkers for T2DM in a clinical trial with 389 human plasma samples. As a result, three of the eight glucose-sensitive peptides (FKDLGEENFK, LDELRDEGK, and KVPQVSTPTLVEVSR) were selected and could be used as potential biomarkers for the early diagnosis of T2DM.
authors.affiliation: School of Life Science, Beijing Institute of Technology, Beijing, China.
authors.forename: Mei
authors.initials: M
authors.lastname: Zhang
authors.forename: Wei
authors.initials: W
authors.lastname: Xu
authors.forename: Yulin
authors.initials: Y
authors.lastname: Deng
mesh_terms: D000328:Adult; D000368:Aged; D000595:Amino Acid Sequence; D015415:Biomarkers / Q000097:blood*; D002853:Chromatography, Liquid / Q000379:methods; D003924:Diabetes Mellitus, Type 2 / Q000097:blood / Q000175:diagnosis*; D042241:Early Diagnosis; D005260:Female; D018149:Glucose Intolerance / Q000175:diagnosis; D017127:Glycation End Products, Advanced; D006031:Glycosylation; D006801:Humans; D008297:Male; D008875:Middle Aged; D010103:Oxygen Isotopes; D010446:Peptide Fragments / Q000097:blood; D012709:Serum Albumin / Q000032:analysis* / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000379:methods; D000093423:Glycated Serum Albumin
chemical_list: D015415:Biomarkers; D017127:Glycation End Products, Advanced; D010103:Oxygen Isotopes; D010446:Peptide Fragments; D012709:Serum Albumin; D000093423:Glycated Serum Albumin
references.citation: Ann Hepatol. 2011 Oct-Dec;10(4):493-501
references.pmid: 21911891
references.citation: J Clin Endocrinol Metab. 2006 Nov;91(11):4287-94
references.pmid: 16912127
references.citation: J Proteome Res. 2008 Feb;7(2):698-707
references.pmid: 18092746
references.citation: Diabetes Care. 2004 Feb;27(2):553-91
references.pmid: 14747243
references.citation: Mass Spectrom Rev. 2006 Sep-Oct;25(5):775-97
references.pmid: 16625652
references.citation: Patient Educ Couns. 2010 Aug;80(2):227-32
references.pmid: 20036098
references.citation: Cardiovasc Diabetol. 2011 Dec 22;10:116
references.pmid: 22192330
references.citation: J Proteome Res. 2007 Jun;6(6):2323-30
references.pmid: 17488106
references.citation: Clin Chim Acta. 2011 Aug 17;412(17-18):1606-15
references.pmid: 21601565
references.citation: Biochim Biophys Acta. 2007 Jun;1770(6):933-42
references.pmid: 17368729
references.citation: Protein Cell. 2010 Jul;1(7):675-87
references.pmid: 21203939
references.citation: Clin Biochem. 2012 Oct;45(15):1192-6
references.pmid: 22659058
references.citation: J Proteome Res. 2006 Jul;5(7):1682-7
references.pmid: 16823976
references.citation: Mol Cell Proteomics. 2006 Jun;5(6):1158-70
references.pmid: 16507876
references.citation: Diabetes Care. 2007 Jan;30 Suppl 1:S4-S41
references.pmid: 17192377
references.citation: Diabetes Res Clin Pract. 2011 May;92(2):e31-3
references.pmid: 21281973
references.citation: BMC Cardiovasc Disord. 2012 Jun 07;12:37
references.pmid: 22676422
references.citation: Arch Biochem Biophys. 2012 Jun 1;522(1):17-25
references.pmid: 22516656
references.citation: Diabetes Metab Res Rev. 2000 Jul-Aug;16(4):230-6
references.pmid: 10934451
references.citation: BMB Rep. 2008 Jul 31;41(7):516-22
references.pmid: 18682035
references.citation: Arterioscler Thromb Vasc Biol. 1999 Apr;19(4):972-8
references.pmid: 10195925
references.citation: J Proteome Res. 2011 Jul 1;10(7):3076-88
references.pmid: 21612289
references.citation: Diabetes Care. 2004 Aug;27(8):2067-73
references.pmid: 15277443
references.citation: J Am Soc Mass Spectrom. 2009 Jul;20(7):1303-10
references.pmid: 19362012
references.citation: Hepatology. 2007 Jul;46(1):158-65
references.pmid: 17596883
references.citation: Eur Heart J. 2000 Oct;21(19):1584-90
references.pmid: 10988010
references.citation: Mol Cell Proteomics. 2010 Mar;9(3):579-92
references.pmid: 19955080
references.citation: J Proteome Res. 2009 Feb;8(2):754-69
references.pmid: 19093874
references.citation: Diabet Med. 2011 Sep;28(9):1034-8
references.pmid: 21843301
references.citation: J Biochem Mol Biol. 2004 Jan 31;37(1):35-44
references.pmid: 14761301
references.citation: Clin Chem. 1999 Dec;45(12):2136-41
references.pmid: 10585345
references.citation: J Proteome Res. 2008 May;7(5):2025-32
references.pmid: 18396901
references.citation: J Chromatogr B Analyt Technol Biomed Life Sci. 2010 Jul 15;878(22):1946-52
references.pmid: 20576474
references.citation: Anal Chem. 2011 Jul 15;83(14):5673-80
references.pmid: 21630644
references.citation: Soc Sci Med. 1985;21(12):1329-34
references.pmid: 4095583