title: Antioxidant activity of phosphorylated exopolysaccharide produced by Lactococcus lactis subsp. lactis.
abstract: Exopolysaccharide (EPS) of Lactococcus lactis subsp. lactis was isolated and purified from MRS culture broth. Phosphorylated exopolysaccharide (P-EPS) was synthesized by using the purified EPS and sodium hexametaphosphate (SHMP). The in vitro and in vivo antioxidant activity of EPS and P-EPS was analyzed. Both EPS and P-EPS displayed superoxide anion (O(2-•)), hydroxyl radical (OH•) and DPPH scavenging activity. Catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activity increased in serum and the livers of mice treated with EPS and P-EPS, while malondialdehyde (MDA) levels decreased. P-EPS was shown to prevent the progression of D-galactose-induced oxidative stress in hepatocytes in vivo. P-EPS showed stronger in vitro and in vivo antioxidant activity than EPS.
authors.affiliation: Department of Food Science and Technology, Jinling College, Nanjing Normal University, Nanjing 210097, China.
authors.forename: Yuxing
authors.initials: Y
authors.lastname: Guo
authors.forename: Daodong
authors.initials: D
authors.lastname: Pan
authors.forename: Yangying
authors.initials: Y
authors.lastname: Sun
authors.forename: Lingying
authors.initials: L
authors.lastname: Xin
authors.forename: Hua
authors.initials: H
authors.lastname: Li
authors.forename: Xiaoqun
authors.initials: X
authors.lastname: Zeng
mesh_terms: D000375:Aging / Q000187:drug effects; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001713:Biphenyl Compounds / Q000378:metabolism; D002374:Catalase / Q000097:blood; D016166:Free Radical Scavengers / Q000494:pharmacology; D005690:Galactose / Q000494:pharmacology; D005979:Glutathione Peroxidase / Q000097:blood; D017665:Hydroxyl Radical / Q000378:metabolism; D013294:Lactococcus lactis / Q000737:chemistry*; D008099:Liver / Q000187:drug effects / Q000201:enzymology / Q000648:ultrastructure; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D051379:Mice; D008813:Mice, Inbred ICR; D018384:Oxidative Stress / Q000187:drug effects; D010766:Phosphorylation / Q000187:drug effects; D010851:Picrates / Q000378:metabolism; D011134:Polysaccharides / Q000494:pharmacology*; D020011:Protective Agents / Q000494:pharmacology; D013055:Spectrophotometry, Infrared; D013056:Spectrophotometry, Ultraviolet; D013482:Superoxide Dismutase / Q000097:blood; D013481:Superoxides
chemical_list: D000975:Antioxidants; D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D010851:Picrates; D011134:Polysaccharides; D020011:Protective Agents; D013481:Superoxides; D017665:Hydroxyl Radical; D008315:Malondialdehyde; C004931:1,1-diphenyl-2-picrylhydrazyl; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005690:Galactose
keywords: Antioxidation activity; Exopolysaccharide; Lactococcus lactis subsp. lactis; Phosphorylate