title: Molecular cloning and expression analysis of interleukin (IL)-15 and IL-15 receptor α from rock bream, Oplegnathus fasciatus.
abstract: Mammalian interleukin (IL)-15 plays an important role in the activation of CD8(+) T cells and natural killer (NK) cells along with its receptor α (IL-15Rα). To understand the potential roles of IL-15 and IL-15Rα in fish, we identified IL-15 and IL-15Rα cDNA from rock bream (Oplegnathus fasciatus) and investigated their gene expression profiles after bacterial and viral infection. Coding regions of rock bream (Rb) IL-15 and RbIL-15Rα cDNAs were 534 and 402 bp encoding 177 and 133 amino acid residues, respectively. The sushi domain of IL-15Rα was highly conserved between rock bream and other species. Unlike other IL-15Rαs, RbIL-15Rα does not have a transmembrane region. Gene expression of RbIL-15 and RbIL-15Rα was widely expressed in different tissues of healthy fish, especially immune-related tissues. RbIL-15 and RbIL-15Rα were highly induced in the kidney and spleen after infection with Edwardsiella tarda, Streptococcus iniae and red seabream iridovirus. Gene expression patterns of RbIL-15 and RbIL-15Rα were similar in the kidney and spleen after pathogen infection. However, these genes were differentially induced in the liver after pathogen infection. These results suggest that the different responses of RbIL-15 and RbIL-15Rα to pathogen infection may be induced by different tissues or cell types.
authors.affiliation: Department of Marine Biology & Aquaculture, College of Marine Science, Gyeongsang National University, 445, Inpyong-dong, Tongyeong, Gyeongnam 650-160, Republic of Korea.
authors.forename: Jin-Sol
authors.initials: JS
authors.lastname: Bae
authors.forename: Sang Hee
authors.initials: SH
authors.lastname: Shim
authors.forename: Seong Don
authors.initials: SD
authors.lastname: Hwang
authors.forename: Ju-Won
authors.initials: JW
authors.lastname: Kim
authors.forename: Dae-Won
authors.initials: DW
authors.lastname: Park
authors.forename: Chan-Il
authors.initials: CI
authors.lastname: Park
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics / Q000378:metabolism; D020609:Edwardsiella tarda / Q000502:physiology; D029941:Fish Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D005786:Gene Expression Regulation; D007274:Injections, Intraperitoneal / Q000662:veterinary; D019409:Interleukin-15 / Q000235:genetics* / Q000378:metabolism; D053720:Interleukin-15 Receptor alpha Subunit / Q000235:genetics* / Q000378:metabolism; D007497:Iridoviridae / Q000502:physiology; D008969:Molecular Sequence Data; D009928:Organ Specificity; D010473:Perciformes / Q000235:genetics* / Q000276:immunology* / Q000378:metabolism; D010802:Phylogeny; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary; D016415:Sequence Alignment / Q000662:veterinary; D013291:Streptococcus / Q000502:physiology
chemical_list: D018076:DNA, Complementary; D029941:Fish Proteins; D019409:Interleukin-15; D053720:Interleukin-15 Receptor alpha Subunit; D012333:RNA, Messenger
keywords: Edwardsiella tarda; Interleukin-15; Interleukin-15 receptor alpha; Red seabream iridovirus; Streptococcus iniae