title: Comparison of the FDA-approved CDC DENV-1-4 real-time reverse transcription-PCR with a laboratory-developed assay for dengue virus detection and serotyping.
abstract: Dengue virus (DENV) is the agent of the most common vector-borne disease worldwide. Using 199 clinical samples collected from Nicaragua and Sri Lanka, a laboratory-developed DENV multiplex real-time reverse transcription-PCR (rRT-PCR) proved more clinically sensitive than the FDA-approved CDC assay for DENV serotypes 1 to 4 when measured against a composite reference standard, with sensitivities of 97.4% versus 87.1%, respectively.
authors.affiliation: Division of Infectious Diseases and Geographic Medicine, Stanford University School of Medicine, Stanford, California, USA.
authors.forename: Jesse J
authors.initials: JJ
authors.lastname: Waggoner
authors.forename: Janaki
authors.initials: J
authors.lastname: Abeynayake
authors.forename: Malaya K
authors.initials: MK
authors.lastname: Sahoo
authors.forename: Lionel
authors.initials: L
authors.lastname: Gresh
authors.forename: Yolanda
authors.initials: Y
authors.lastname: Tellez
authors.forename: Karla
authors.initials: K
authors.lastname: Gonzalez
authors.forename: Gabriela
authors.initials: G
authors.lastname: Ballesteros
authors.forename: Frances P
authors.initials: FP
authors.lastname: Guo
authors.forename: Angel
authors.initials: A
authors.lastname: Balmaseda
authors.forename: Kumudu
authors.initials: K
authors.lastname: Karunaratne
authors.forename: Eva
authors.initials: E
authors.lastname: Harris
authors.forename: Benjamin A
authors.initials: BA
authors.lastname: Pinsky
mesh_terms: D003715:Dengue / Q000175:diagnosis* / Q000821:virology*; D003716:Dengue Virus / Q000145:classification* / Q000302:isolation & purification*; D006801:Humans; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D009527:Nicaragua; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D012703:Serotyping; D013188:Sri Lanka; D014773:Virology / Q000379:methods*
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