title: MicroRNA-145 function as a cell growth repressor by directly targeting c-Myc in human ovarian cancer.
abstract: MiR-145 is reported to be significantly down-regulated in ovarian cancer. This study was aimed at elucidating the roles of miR-145 in regulating the biological behavior of epithelial ovarian cancer (EOC) cells. In this report, we find out that up-regulation of miR-145 in OVCAR-3 and SKOV-3 cells inhibit cell proliferation and promote cell apoptosis. We show that miR-145 directly target the c-Myc 3'-UTR. Moreover, ectopic expression of c-Myc reduces the inhibition of cell proliferation caused by miR-145 transfection. Cell cycle assay showed that up-regulation of miR-145 reduces S phase population, and restoration of c-Myc can rescue this reduction. These findings indicate that miR-145 inhibits cell proliferation and promotes cell apoptosis by targeting c-Myc 3'-UTR. Therefore, the result indicated that miR- 145 could be used as a potential therapeutic target in ovarian cancer.
authors.affiliation: Department of Gynecology and Obstetrics, The Military General Hospital of Beijing PLA, No. 5 Nan Men Cang, Dongcheng District, Beijing 100700, PR China. wanghuibjing@sina.cn.
authors.forename: Wenjing
authors.initials: W
authors.lastname: Zhang
authors.forename: Qian
authors.initials: Q
authors.lastname: Wang
authors.forename: Minglian
authors.initials: M
authors.lastname: Yu
authors.forename: Nan
authors.initials: N
authors.lastname: Wu
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
mesh_terms: D000077216:Carcinoma, Ovarian Epithelial; D002453:Cell Cycle / Q000235:genetics; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015536:Down-Regulation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D009375:Neoplasms, Glandular and Epithelial / Q000235:genetics / Q000378:metabolism; D010051:Ovarian Neoplasms / Q000235:genetics* / Q000378:metabolism; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics* / Q000378:metabolism
chemical_list: C541010:MIRN145 microRNA, human; D035683:MicroRNAs; D016271:Proto-Oncogene Proteins c-myc