title: [GC-MS determination of metabolites in rat kidneys].
abstract: OBJECTIVE
  To establish a method to determine the metabolites in rat kidney tissues by gas chromatography-mass spectrometry (GC-MS) combined with chemometric techniques.
  
  
  METHODS
  Metabolites were separated and identified on HP-5MS column (30 m × 0.25 μm × 0.25 mm). The initial column temperature was 100 Celsius degree lasting 3 min, and then programmed at 8 Celsius degree/ min to 300 Celsius degree, maintaining at this temperature for 6 min. The internal standard was heptadecanoic acid. The grinded kidney tissue was exacted by methanol. The supernatant was dried by nitrogen. After the oximation and derivation, the supernatant was analyzed by GC-MS. The overlapped peaks were resolved into pure chromatogram and mass spectra with chemometric techniques. Qualitative analysis was performed by comparing the obtained pure mass spectra with those in NIST mass spectra database and certificated by the standards and the references. The internal method was used for semi-quantitation.
  
  
  RESULTS
  A total of 53 compounds were identified. The main constitutions in the kidney tissue were amino acids, saccharides, fatty acids and urea.
  
  
  CONCLUSIONS
  The combination of methods is rapid and accurate for the analysis of metabolites in the kidney tissue, which provides more information for further study of metabonomics in kidney tissues.
authors.affiliation: Department of Pharmacy, Xiangya Hospital, Central South University, Changsha 410008, China.
authors.forename: Shao
authors.initials: S
authors.lastname: Liu
authors.forename: Fangjie
authors.initials: F
authors.lastname: Wang
authors.forename: Wenjuan
authors.initials: W
authors.lastname: Mei
authors.forename: Lijian
authors.initials: L
authors.lastname: Tao
mesh_terms: D000596:Amino Acids / Q000032:analysis; D000818:Animals; D005227:Fatty Acids / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry; D007668:Kidney / Q000378:metabolism*; D055442:Metabolome; D055432:Metabolomics / Q000379:methods*; D051381:Rats; D014508:Urea / Q000032:analysis*
chemical_list: D000596:Amino Acids; D005227:Fatty Acids; D014508:Urea