title: Allergenicity potential of red kidney bean (Phaseolus vulgaris L.) proteins in orally treated BALB/c mice and passively sensitized RBL-2H3 cells.
abstract: Red kidney bean (Phaseolus vulgaris L.) is one the most commonly consumed legumes that requires an in depth understanding of its allergenicity. Therefore, the aim of this study was to explore the allergenicity of red kidney bean proteins following oral exposure in BALB/c mice and elucidate the levels of Th1/Th2 transcription factors induced by red kidney bean proteins in rat basophilic leukemia cells (RBL-2H3 cells) passively sensitized with the sera of red kidney bean sensitized mice. Red kidney bean proteins showed enhanced levels of total and specific IgE, anaphylactic symptoms, thymic stromal lymphopoietin (TSLP) and peritoneal albumin over control. Enhanced release of β-hexosaminidase along with up regulated expressions of GATA-3, STAT-6, T-bet, c-MAF and NFAT were observed in the RBL-2H3 cells exposed with red kidney bean proteins when compared to that of the controls. Taken together, exposure of red kidney bean proteins may cause allergic symptoms in mice and the ambivalent effect on Th2/Th1 transcription factors in RBL-2H3 cells.
authors.affiliation: Food Toxicology Division, CSIR-Indian Institute of Toxicology Research (Formerly - Industrial Toxicology Research Centre), Mahatma Gandhi Marg, P.O. Box No. 80, Lucknow 226 001, India; Department of Biotechnology, Faculty of Science, Jamia Hamdard, Hamdard Nagar, New Delhi 110 062, India. Electronic address: sandeep_sant@yahoo.co.in.
authors.forename: Sandeep
authors.initials: S
authors.lastname: Kumar
authors.forename: Akanksha
authors.initials: A
authors.lastname: Sharma
authors.forename: Alok K
authors.initials: AK
authors.lastname: Verma
authors.forename: B P
authors.initials: BP
authors.lastname: Chaudhari
authors.forename: Mukul
authors.initials: M
authors.lastname: Das
authors.forename: S K
authors.initials: SK
authors.lastname: Jain
authors.forename: Premendra D
authors.initials: PD
authors.lastname: Dwivedi
mesh_terms: D000707:Anaphylaxis / Q000276:immunology*; D000818:Animals; D015153:Blotting, Western; D045744:Cell Line, Tumor; D005260:Female; D005512:Food Hypersensitivity / Q000276:immunology*; D050990:GATA3 Transcription Factor / Q000032:analysis / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D050778:NFATC Transcription Factors / Q000032:analysis / Q000276:immunology; D027805:Phaseolus / Q000276:immunology*; D010940:Plant Proteins / Q000276:immunology*; D051257:Proto-Oncogene Proteins c-maf / Q000032:analysis / Q000276:immunology; D011897:Random Allocation; D051381:Rats; D013047:Specific Pathogen-Free Organisms; D020825:T-Box Domain Proteins / Q000032:analysis / Q000276:immunology; D018417:Th1 Cells / Q000276:immunology*; D018418:Th2 Cells / Q000276:immunology*; D001619:beta-N-Acetylhexosaminidases / Q000032:analysis / Q000276:immunology; D000098815:T-bet Transcription Factor
chemical_list: D050990:GATA3 Transcription Factor; C494748:Gata3 protein, mouse; C495608:Maf protein, mouse; D050778:NFATC Transcription Factors; D010940:Plant Proteins; D051257:Proto-Oncogene Proteins c-maf; D020825:T-Box Domain Proteins; D000098815:T-bet Transcription Factor; D001619:beta-N-Acetylhexosaminidases
keywords: Allergic mediators; Allergy; Legumes; Thymic stromal lymphopoietin; Transcription factors