title: Generation of human neutralizing monoclonal antibodies against the 2009 pandemic H1N1 virus from peripheral blood memory B lymphocytes.
abstract: The 2009 H1N1 influenza pandemic demonstrated the significance of a global health threat to human beings. Although pandemic H1N1 vaccines have been rapidly developed, passive serotherapy may offer superior immediate protection against infections in children, the elderly and immune-compromised patients during an influenza pandemic. Here, we applied a novel strategy based on Epstein-Barr virus (EBV)-immortalized peripheral blood memory B cells to screen high viral neutralizing monoclonal antibodies (MAbs) from individuals vaccinated with the 2009 pandemic H1N1 vaccine PANFLU.1. Through a massive screen of 13 090 immortalized memory B-cell clones from three selected vaccinees, seven MAbs were identified with both high viral neutralizing capacities and hemagglutination inhibition (HAI) activities against the 2009 pandemic H1N1 viruses. These MAbs may have important clinical implications for passive serotherapy treatments of infected patients with severe respiratory syndrome, especially children, the elderly and immunodeficient individuals. Our successful strategy for generating high-affinity MAbs from EBV-immortalized peripheral blood memory B cells may also be applicable to other infectious or autoimmune diseases.
authors.affiliation: Department of Immunology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and School of Basic Medicine, Peking UnionMedical College, State Key Laboratory of Medical Molecular Biology, Beijing, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Wang
authors.forename: Chi
authors.initials: C
authors.lastname: Ma
authors.forename: Yanlai
authors.initials: Y
authors.lastname: Lu
authors.forename: Xu
authors.initials: X
authors.lastname: Ji
authors.forename: Yongsheng
authors.initials: Y
authors.lastname: Pang
authors.forename: Fang
authors.initials: F
authors.lastname: Hua
authors.forename: Lianxian
authors.initials: L
authors.lastname: Cui
authors.forename: Denian
authors.initials: D
authors.lastname: Ba
authors.forename: Wei
authors.initials: W
authors.lastname: He
mesh_terms: D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000097:blood / Q000302:isolation & purification; D057134:Antibodies, Neutralizing / Q000096:biosynthesis* / Q000097:blood / Q000302:isolation & purification; D000914:Antibodies, Viral / Q000096:biosynthesis* / Q000097:blood / Q000302:isolation & purification; D001402:B-Lymphocytes / Q000276:immunology / Q000378:metabolism* / Q000821:virology; D002681:China / Q000453:epidemiology; D006385:Hemagglutination Inhibition Tests; D004854:Herpesvirus 4, Human / Q000235:genetics / Q000276:immunology; D057166:High-Throughput Screening Assays; D006801:Humans; D007156:Immunologic Memory; D053118:Influenza A Virus, H1N1 Subtype / Q000276:immunology; D007252:Influenza Vaccines / Q000008:administration & dosage; D007251:Influenza, Human / Q000097:blood / Q000453:epidemiology / Q000276:immunology / Q000517:prevention & control*; D009500:Neutralization Tests; D058873:Pandemics; D014611:Vaccination
chemical_list: D000911:Antibodies, Monoclonal; D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D007252:Influenza Vaccines
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