title: Aberrant histone modification in peripheral blood B cells from patients with systemic sclerosis.
abstract: OBJECTIVE
  To investigate alterations in histone modifications in B cells and their role in the pathogenesis of systemic sclerosis (SSc).
  
  
  METHODS
  Global histone H3/H4 acetylation and H3K4/H3K9 methylation in B cells of SSc were tested by EpiQuik™ assay kits. Related histone modifier enzymes were measured by RT-PCR and Western blot.
  
  
  RESULTS
  Global histone H4 hyperacetylation and global histone H3K9 hypomethylation were observed in SSc B cells compared with controls. Expression of JHDM2A was significantly increased but HDAC2, HDAC7, and, SUV39H2 were significantly down-regulated in SSc B cells relative to controls. Global histone H4 acetylation and the expression of HDAC2 were negatively correlated. Global histone H3K9 methylation and the expression of SUV39H2 protein were positively correlated. Global H4 acetylation was positively correlated with disease activity and expression of HDAC2 protein was negatively correlated with skin thickness.
  
  
  CONCLUSIONS
  Histone modifications were altered in B cells in SSc correlating with skin thickness and disease activity.
authors.affiliation: Department of Dermatology, Second Xiangya Hospital, Central South University, 139 Ren-Min Road, Changsha 410011, China.
authors.forename: YaoYao
authors.initials: Y
authors.lastname: Wang
authors.forename: Yan
authors.initials: Y
authors.lastname: Yang
authors.forename: YangYang
authors.initials: Y
authors.lastname: Luo
authors.forename: YongXin
authors.initials: Y
authors.lastname: Yin
authors.forename: Qing
authors.initials: Q
authors.lastname: Wang
authors.forename: YaPing
authors.initials: Y
authors.lastname: Li
authors.forename: Takuro
authors.initials: T
authors.lastname: Kanekura
authors.forename: JiuCun
authors.initials: J
authors.lastname: Wang
authors.forename: GongPing
authors.initials: G
authors.lastname: Liang
authors.forename: Ming
authors.initials: M
authors.lastname: Zhao
authors.forename: QianJin
authors.initials: Q
authors.lastname: Lu
authors.forename: Rong
authors.initials: R
authors.lastname: Xiao
mesh_terms: D000107:Acetylation; D000328:Adult; D001402:B-Lymphocytes / Q000378:metabolism*; D005260:Female; D056464:Histone Deacetylase 2 / Q000235:genetics / Q000378:metabolism; D006655:Histone Deacetylases / Q000235:genetics / Q000378:metabolism; D011495:Histone-Lysine N-Methyltransferase / Q000235:genetics / Q000378:metabolism; D006657:Histones / Q000378:metabolism*; D006801:Humans; D056484:Jumonji Domain-Containing Histone Demethylases / Q000235:genetics / Q000378:metabolism; D008297:Male; D008745:Methylation; D008875:Middle Aged; D012333:RNA, Messenger / Q000378:metabolism; D012595:Scleroderma, Systemic / Q000378:metabolism* / Q000473:pathology
chemical_list: D006657:Histones; D012333:RNA, Messenger; D056484:Jumonji Domain-Containing Histone Demethylases; D011495:Histone-Lysine N-Methyltransferase; C515701:SUV39H2 protein, human; C533423:HDAC2 protein, human; C543273:HDAC7 protein, human; D056464:Histone Deacetylase 2; D006655:Histone Deacetylases
keywords: Acetylation; B cells; Histone modification; Methylation; Systemic sclerosis