title: Crystallization and preliminary X-ray analysis of monalysin, a novel β-pore-forming toxin from the entomopathogen Pseudomonas entomophila.
abstract: Monalysin was recently described as a novel pore-forming toxin (PFT) secreted by the Drosophila pathogen Pseudomonas entomophila. Recombinant monalysin is multimeric in solution, whereas PFTs are supposed to be monomeric until target membrane association. Monalysin crystals were obtained by the hanging-drop vapour-diffusion method using PEG 8000 as precipitant. Preliminary X-ray diffraction analysis revealed that monalysin crystals belonged to the monoclinic space group C2, with unit-cell parameters a = 162.4, b = 146.2, c = 144.4 Å, β = 122.8°, and diffracted to 2.85 Å resolution using synchrotron radiation. Patterson self-rotation analysis and Matthews coefficient calculation indicate that the asymmetric unit contains nine copies of monalysin. Heavy-atom derivative data were collected and a Ta6Br14 cluster derivative data set confirmed the presence of ninefold noncrystallographic symmetry.
authors.affiliation: Architecture et Fonction des Macromolécules Biologiques (AFMB), UMR 7257 CNRS et Aix-Marseille Université, Marseille, France.
authors.forename: Maryline
authors.initials: M
authors.lastname: Blemont
authors.forename: Renaud
authors.initials: R
authors.lastname: Vincentelli
authors.forename: Christine
authors.initials: C
authors.lastname: Kellenberger
authors.forename: Onya
authors.initials: O
authors.lastname: Opota
authors.forename: Bruno
authors.initials: B
authors.lastname: Lemaitre
authors.forename: Alain
authors.initials: A
authors.lastname: Roussel
authors.forename: Philippe
authors.initials: P
authors.lastname: Leone
mesh_terms: D001427:Bacterial Toxins / Q000737:chemistry* / Q000302:isolation & purification; D003460:Crystallization; D018360:Crystallography, X-Ray; D052899:Pore Forming Cytotoxic Proteins / Q000737:chemistry* / Q000302:isolation & purification; D011549:Pseudomonas
chemical_list: D001427:Bacterial Toxins; D052899:Pore Forming Cytotoxic Proteins
keywords: Pseudomonas entomophila; monalysin; pore-forming toxins
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