title: A new nidovirus (NamDinh virus NDiV): Its ultrastructural characterization in the C6/36 mosquito cell line.
abstract: We describe the ultrastructure of the NamDinh virus (NDiV), a new member of the order Nidovirales grown in the C6/36 mosquito cell line. Uninfected and NDiV-infected cells were investigated by electron microscopy 24-48 h after infection. The results show that the viral nucleocapsid-like particles form clusters concentrated in the vacuoles, the endoplasmic reticulum, and are scattered in the cytoplasm. Mature virions of NDiV were released as budding particles on the cell surface where viral components appear to lie beneath and along the plasma membrane. Free homogeneous virus particles were obtained by ultracentrifugation on sucrose gradients of culture fluids. The size of the round-shaped particles with a complete internal structure was 80 nm in diameter. This is the first study to provide information on the morphogenesis and ultrastructure of the first insect nidovirus NDiV, a missing evolutionary link in the emergence of the viruses with the largest RNA genomes.
authors.affiliation: National Institute of Hygiene and Epidemiology, 1 Yersin Street, Hai Ba Trung District, Hanoi, Viet Nam. ngtthuy02@yahoo.com
authors.forename: Nguyen Thanh
authors.initials: NT
authors.lastname: Thuy
authors.forename: Tran Quang
authors.initials: TQ
authors.lastname: Huy
authors.forename: Phan Thi
authors.initials: PT
authors.lastname: Nga
authors.forename: Kouichi
authors.initials: K
authors.lastname: Morita
authors.forename: Irene
authors.initials: I
authors.lastname: Dunia
authors.forename: Lucio
authors.initials: L
authors.lastname: Benedetti
mesh_terms: D000818:Animals; D002460:Cell Line; D002462:Cell Membrane / Q000821:virology; D009033:Culicidae; D003593:Cytoplasm / Q000821:virology; D008854:Microscopy, Electron; D028381:Nidovirales / Q000302:isolation & purification* / Q000502:physiology / Q000648:ultrastructure*; D015388:Organelles / Q000648:ultrastructure / Q000821:virology; D014771:Virion / Q000648:ultrastructure; D057074:Virus Release
keywords: Electron microscopy; Immunogold; NDiV; NamDinh virus; Nidovirus; Ultrastructure
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