title: Specific mutations in H5N1 mainly impact the magnitude and velocity of the host response in mice.
abstract: BACKGROUND
  Influenza infection causes respiratory disease that can lead to death. The complex interplay between virus-encoded and host-specific pathogenicity regulators - and the relative contributions of each toward viral pathogenicity - is not well-understood.
  
  
  RESULTS
  By analyzing a collection of lung samples from mice infected by A/Vietnam/1203/2004 (H5N1; VN1203), we characterized a signature of transcripts and proteins associated with the kinetics of the host response. Using a new geometrical representation method and two criteria, we show that inoculation concentrations and four specific mutations in VN1203 mainly impact the magnitude and velocity of the host response kinetics, rather than specific sets of up- and down- regulated genes. We observed analogous kinetic effects using lung samples from mice infected with A/California/04/2009 (H1N1), and we show that these effects correlate with morbidity and viral titer.
  
  
  CONCLUSIONS
  We have demonstrated the importance of the kinetics of the host response to H5N1 pathogenesis and its relationship with clinical disease severity and virus replication. These kinetic properties imply that time-matched comparisons of 'omics profiles to viral infections give limited views to differentiate host-responses. Moreover, these results demonstrate that a fast activation of the host-response at the earliest time points post-infection is critical for protective mechanisms against fast replicating viruses.
authors.affiliation: Department of Microbiology, University of Washington, Seattle, WA 98195 USA.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Tchitchek
authors.forename: Amie J
authors.initials: AJ
authors.lastname: Eisfeld
authors.forename: Jennifer
authors.initials: J
authors.lastname: Tisoncik-Go
authors.forename: Laurence
authors.initials: L
authors.lastname: Josset
authors.forename: Lisa E
authors.initials: LE
authors.lastname: Gralinski
authors.forename: Christophe
authors.initials: C
authors.lastname: Bécavin
authors.forename: Susan C
authors.initials: SC
authors.lastname: Tilton
authors.forename: Bobbie-Jo
authors.initials: BJ
authors.lastname: Webb-Robertson
authors.forename: Martin T
authors.initials: MT
authors.lastname: Ferris
authors.forename: Allison L
authors.initials: AL
authors.lastname: Totura
authors.forename: Chengjun
authors.initials: C
authors.lastname: Li
authors.forename: Gabriele
authors.initials: G
authors.lastname: Neumann
authors.forename: Thomas O
authors.initials: TO
authors.lastname: Metz
authors.forename: Richard D
authors.initials: RD
authors.lastname: Smith
authors.forename: Katrina M
authors.initials: KM
authors.lastname: Waters
authors.forename: Ralph
authors.initials: R
authors.lastname: Baric
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Kawaoka
authors.forename: Michael G
authors.initials: MG
authors.lastname: Katze
mesh_terms: D000818:Animals; D005260:Female; D054884:Host-Pathogen Interactions / Q000235:genetics*; D053118:Influenza A Virus, H1N1 Subtype / Q000235:genetics / Q000502:physiology; D053124:Influenza A Virus, H5N1 Subtype / Q000235:genetics* / Q000502:physiology*; D007700:Kinetics; D008168:Lung / Q000821:virology; D051379:Mice; D008810:Mice, Inbred C57BL; D009154:Mutation; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013045:Species Specificity; D019562:Viral Load / Q000235:genetics; D014764:Viral Proteins / Q000235:genetics; D014779:Virus Replication / Q000235:genetics
chemical_list: D012333:RNA, Messenger; D014764:Viral Proteins
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