title: Glycinyl-histidinyl-serine (GHS), a novel rapeseed protein-derived peptide has blood pressure-lowering effect in spontaneously hypertensive rats.
abstract: A novel antihypertensive peptide (Gly-His-Ser or GHS) with dual inhibition of angiotensin I-converting enzyme (ACE) and renin activities was isolated from the 3 kDa membrane ultrafiltration permeate of a pepsin+pancreatin rapeseed protein digest. The IC50 values of GHS were 0.52 ± 0.01 mg/mL and 0.32 ± 0.01 mg/mL for ACE and renin inhibitions, respectively, which are 1.5 times the ACE inhibition and 3.5 times the renin inhibition of the 3 kDa permeate. Oral administration (30 mg/kg body weight) to spontaneously hypertensive rats showed GHS to be an effective hypotensive agent with maximum blood pressure reduction of -17.29 ± 2.47 mmHg after 6 h. In contrast, the 3 kDa permeate exhibited a maximum of -21.29 ± 9.29 mmHg after 4 h, although at a relatively higher dose of 100 mg/kg body weight). GHS inhibited ACE and renin activities noncompetitively, but the renin inhibition became uncompetitive at a higher peptide concentration.
authors.affiliation: College of Food Science and Engineering, Nanjing University of Finance and Economics , Nanjing, Jiangsu, People's Republic of China 210003.
authors.forename: Rong
authors.initials: R
authors.lastname: He
authors.forename: Sunday A
authors.initials: SA
authors.lastname: Malomo
authors.forename: Abraham T
authors.initials: AT
authors.lastname: Girgih
authors.forename: Xingrong
authors.initials: X
authors.lastname: Ju
authors.forename: Rotimi E
authors.initials: RE
authors.lastname: Aluko
mesh_terms: D000806:Angiotensin-Converting Enzyme Inhibitors; D000818:Animals; D000959:Antihypertensive Agents / Q000008:administration & dosage*; D029741:Brassica rapa / Q000737:chemistry*; D004305:Dose-Response Relationship, Drug; D006801:Humans; D006973:Hypertension / Q000188:drug therapy*; D008297:Male; D009842:Oligopeptides / Q000008:administration & dosage* / Q000302:isolation & purification; D010940:Plant Proteins / Q000737:chemistry*; D051381:Rats; D011918:Rats, Inbred SHR; D000092502:Renin Inhibitors
chemical_list: D000806:Angiotensin-Converting Enzyme Inhibitors; D000959:Antihypertensive Agents; D009842:Oligopeptides; D010940:Plant Proteins; D000092502:Renin Inhibitors; C000589228:glycyl-histidyl-serine