title: Novel diagnostic procedure for determining metastasis to sentinel lymph nodes in breast cancer using a semi-dry dot-blot method.
abstract: We developed an easy, quick and cost-effective detection method for lymph node metastasis called the semi-dry dot-blot (SDB) method, which visualizes the presence of cancer cells with washing of sectioned lymph nodes by anti-pancytokeratin antibody, modifying dot-blot technology. We evaluated the validity and efficacy of the SDB method for the diagnosis of lymph node metastasis in a clinical setting (Trial 1). To evaluate the validity of the SDB method in clinical specimens, 180 dissected lymph nodes from 29 cases, including breast, gastric and colorectal cancer, were examined. Each lymph node was sliced at the maximum diameter and the sensitivity, specificity and accuracy of the SDB method were determined and compared with the final pathology report. Metastasis was detected in 32 lymph nodes (17.8%), and the sensitivity, specificity and accuracy of the SDB method were 100, 98.0 and 98.3%, respectively (Trial 2). To evaluate the efficacy of the SDB method in sentinel lymph node (SLN) biopsy, 174 SLNs from 100 cases of clinically node-negative breast cancer were analyzed. Each SLN was longitudinally sliced at 2-mm intervals and the sensitivity, specificity, accuracy and time required for the SDB method were determined and compared with the intraoperative pathology report. Metastasis was detected in 15 SLNs (8.6%), and the sensitivity, specificity, accuracy and mean required time of the SDB method were 93.3, 96.9, 96.6 and 43.3 min, respectively. The SDB method is a novel and reliable modality for the intraoperative diagnosis of SLN metastasis.
authors.affiliation: Department of Surgical Oncology, Nagasaki University Hospital, Nagasaki, Japan.
authors.forename: Ryota
authors.initials: R
authors.lastname: Otsubo
authors.forename: Masahiro
authors.initials: M
authors.lastname: Oikawa
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Hirakawa
authors.forename: Kenichiro
authors.initials: K
authors.lastname: Shibata
authors.forename: Kuniko
authors.initials: K
authors.lastname: Abe
authors.forename: Tomayoshi
authors.initials: T
authors.lastname: Hayashi
authors.forename: Naoe
authors.initials: N
authors.lastname: Kinoshita
authors.forename: Kazuto
authors.initials: K
authors.lastname: Shigematsu
authors.forename: Toshiko
authors.initials: T
authors.lastname: Hatachi
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Yano
authors.forename: Megumi
authors.initials: M
authors.lastname: Matsumoto
authors.forename: Katsunori
authors.initials: K
authors.lastname: Takagi
authors.forename: Tomoshi
authors.initials: T
authors.lastname: Tsuchiya
authors.forename: Koichi
authors.initials: K
authors.lastname: Tomoshige
authors.forename: Masahiro
authors.initials: M
authors.lastname: Nakashima
authors.forename: Hideki
authors.initials: H
authors.lastname: Taniguchi
authors.forename: Takeyuki
authors.initials: T
authors.lastname: Omagari
authors.forename: Noriaki
authors.initials: N
authors.lastname: Itoyanagi
authors.forename: Takeshi
authors.initials: T
authors.lastname: Nagayasu
mesh_terms: D001365:Axilla; D014408:Biomarkers, Tumor / Q000378:metabolism*; D001943:Breast Neoplasms / Q000378:metabolism / Q000473:pathology* / Q000601:surgery; D018270:Carcinoma, Ductal, Breast / Q000378:metabolism / Q000556:secondary* / Q000601:surgery; D002285:Carcinoma, Intraductal, Noninfiltrating / Q000378:metabolism / Q000473:pathology* / Q000601:surgery; D018275:Carcinoma, Lobular / Q000378:metabolism / Q000556:secondary* / Q000601:surgery; D003110:Colonic Neoplasms / Q000378:metabolism / Q000473:pathology* / Q000601:surgery; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D015151:Immunoblotting; D007124:Immunoenzyme Techniques; D008197:Lymph Node Excision; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D011379:Prognosis; D011446:Prospective Studies; D012680:Sensitivity and Specificity; D021701:Sentinel Lymph Node Biopsy; D013274:Stomach Neoplasms / Q000378:metabolism / Q000473:pathology* / Q000601:surgery
chemical_list: D014408:Biomarkers, Tumor
keywords: breast cancer; cytokeratin; metastasis; semi-dry dot-blot; sentinel lymph node