title: Interleukin 10 (IL10) and transforming growth factor β1 (TGFβ1) gene polymorphisms in persistent IgE-mediated cow's milk allergy.
abstract: OBJECTIVE
  The aim of this cross-sectional study was to evaluate whether interleukin 10 (IL10) and transforming growth factor β1 (TGFβ1) gene polymorphisms were associated with persistent IgE-mediated cow's milk allergy in 50 Brazilian children. The diagnostic criteria were anaphylaxis triggered by cow's milk or a positive double-blind, placebo-controlled food challenge. Tolerance was defined as the absence of a clinical response to a double-blind, placebo-controlled food challenge or cow's milk exposure.
  
  
  METHODS
  The genomic DNA of the 50 patients and 224 healthy controls (HCs) was used to investigate five IL10 gene polymorphisms (-3575A/T, -2849A/G, -2763A/C, -1082G/A, -592C/A) and one TGFβ1 polymorphism (-509C/T).
  
  
  RESULTS
  Among the five IL10 polymorphisms analyzed, homozygosis for the G allele at the -1082 position was significantly higher in the patients compared with the healthy controls (p=0.027) and in the persistent cow's milk allergy group compared with the healthy controls (p=0.001).
  
  
  CONCLUSIONS
  Homozygosis for the G allele at the IL10 -1082G/A polymorphism is associated with the persistent form of cow's milk allergy.
authors.affiliation: Allergy and Immunology Unit, Department of Pediatrics, Faculdade de Medicina, Universidade de São Paulo, São Paulo, SP, Brazil. cristina.jacob@icr.usp.br
authors.forename: Cristina Miuki Abe
authors.initials: CM
authors.lastname: Jacob
authors.forename: Antonio Carlos
authors.initials: AC
authors.lastname: Pastorino
authors.forename: Thelma Suely
authors.initials: TS
authors.lastname: Okay
authors.forename: Ana Paula B M
authors.initials: AP
authors.lastname: Castro
authors.forename: Andrea Keiko F
authors.initials: AK
authors.lastname: Gushken
authors.forename: Letícia Aki
authors.initials: LA
authors.lastname: Watanabe
authors.forename: Vanessa C Z
authors.initials: VC
authors.lastname: Frucchi
authors.forename: Léa Campos de
authors.initials: LC
authors.lastname: Oliveira
mesh_terms: D001938:Brazil; D016022:Case-Control Studies; D002648:Child; D003430:Cross-Sectional Studies; D005260:Female; D005787:Gene Frequency; D006801:Humans; D007073:Immunoglobulin E / Q000276:immunology*; D016753:Interleukin-10 / Q000235:genetics*; D016015:Logistic Models; D008297:Male; D016269:Milk Hypersensitivity / Q000235:genetics* / Q000276:immunology; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D012307:Risk Factors; D053773:Transforming Growth Factor beta1 / Q000235:genetics*
chemical_list: D053773:Transforming Growth Factor beta1; D016753:Interleukin-10; D007073:Immunoglobulin E
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