title: Binding of pro-migratory serum factors to electrospun PLLA nano-fibers.
abstract: Architecture of the poly(l-lactic acid) (PLLA) scaffolds is known to affect protein affinity and binding strength. Here, we demonstrate that nanofibrous electrospun PLLA scaffolds reversibly absorb the pro-migratory serum factors that stimulate migration of vascular smooth muscle via an NFkB-dependent mechanism. Further, we demonstrate that mesenchymal stem cells seeded on the PLLA scaffolds do not enhance muscle migration but may maintain the ability of induced cells to migrate in an NFkB-independent manner. These findings further support the promising application of PLLA scaffolds for therapeutic angiogenesis and vascular graft engineering.
authors.affiliation: a Department of Biochemistry and Molecular Biology , University of Maryland School of Medicine , 108 N Greene St, BRF 329, Baltimore , MD , 21201 , USA .
authors.forename: Saman
authors.initials: S
authors.lastname: Eghtesad
authors.forename: Maria V
authors.initials: MV
authors.lastname: Nurminskaya
mesh_terms: D000042:Absorption; D001798:Blood Proteins / Q000737:chemistry*; D002465:Cell Movement / Q000187:drug effects; D006801:Humans; D019344:Lactic Acid / Q000737:chemistry* / Q000494:pharmacology; D009131:Muscle, Smooth, Vascular / Q000166:cytology; D057139:Nanofibers / Q000737:chemistry*; D036103:Nanotechnology / Q000379:methods*; D011091:Polyesters; D011108:Polymers / Q000737:chemistry* / Q000494:pharmacology; D015398:Signal Transduction / Q000187:drug effects; D054457:Tissue Scaffolds / Q000737:chemistry
chemical_list: D001798:Blood Proteins; D011091:Polyesters; D011108:Polymers; D019344:Lactic Acid; C033616:poly(lactide)
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