title: Neuronal damage using fluoro-Jade B histofluorescence and gliosis in the gerbil septum submitted to various durations of cerebral ischemia.
abstract: The extent of neuronal damage/death in some brain regions is highly correlated to duration time of transient ischemia. In the present study, we carried out neuronal degeneration/death and glial changes in the septum 4 days after 5, 10, 15, and 20 min of transient cerebral ischemia using gerbils. To examine neuronal damage, Fluoro-Jade B (F-J B, a marker for neuronal degeneration) histofluorescence staining was used. F-J B positive ((+)) cells were detected in the septo-hippocampal nucleus (SHN) of the septum only in the 20 min ischemia-group; the mean number of F-J B(+) neurons was 14.9 ± 2.5/400 μm(2) in a section. Gliosis of astrocytes and microglia was examined using anti-glial fibrillary acidic protein (GFAP) and anti-ionized calcium-binding adapter molecule 1 (Iba-1), respectively. In all the ischemia-groups, GFAP- and Iba-1-immunoreactive astrocytes and microglia, respectively, were increased in number, and apparently tended to be increased in their immunoreactivity. Especially, in the 20 min ischemia-group, the number and immunoreactivity of Iba-immunoreactive microglia was highest and strongest in the ischemic SHN 4 days after ischemia-reperfusion. In brief, our findings showed that neuronal damage/death in the SHN occurred and gliosis was apparently increased in the 20 min ischemia-group at 4 days after ischemia-reperfusion.
authors.affiliation: Department of Emergency Medicine, and Institute of Medical Sciences, School of Medicine, Kangwon National University, Chuncheon, 200-701, South Korea.
authors.forename: Chan Woo
authors.initials: CW
authors.lastname: Park
authors.forename: Jae-Chul
authors.initials: JC
authors.lastname: Lee
authors.forename: Ji Hyeon
authors.initials: JH
authors.lastname: Ahn
authors.forename: Dae Hwan
authors.initials: DH
authors.lastname: Lee
authors.forename: Geum-Sil
authors.initials: GS
authors.lastname: Cho
authors.forename: Bing Chun
authors.initials: BC
authors.lastname: Yan
authors.forename: Joon Ha
authors.initials: JH
authors.lastname: Park
authors.forename: In Hye
authors.initials: IH
authors.lastname: Kim
authors.forename: Hui Young
authors.initials: HY
authors.lastname: Lee
authors.forename: Moo-Ho
authors.initials: MH
authors.lastname: Won
authors.forename: Jun Hwi
authors.initials: JH
authors.lastname: Cho
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000378:metabolism / Q000473:pathology; D048588:Benzoxazines; D002545:Brain Ischemia / Q000150:complications / Q000378:metabolism / Q000473:pathology*; D005452:Fluoresceins / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D005849:Gerbillinae / Q000378:metabolism*; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D005911:Gliosis / Q000150:complications / Q000378:metabolism* / Q000473:pathology*; D008297:Male; D008840:Microfilament Proteins / Q000378:metabolism; D017628:Microglia / Q000378:metabolism / Q000473:pathology; D009419:Nerve Tissue Proteins / Q000378:metabolism; D009474:Neurons / Q000378:metabolism / Q000473:pathology*; D020665:Septum of Brain / Q000378:metabolism / Q000473:pathology*; D013194:Staining and Labeling
chemical_list: D048588:Benzoxazines; D005452:Fluoresceins; D005904:Glial Fibrillary Acidic Protein; D008840:Microfilament Proteins; D009419:Nerve Tissue Proteins; C435731:fluoro jade; C028911:cresyl violet
references.citation: J Neurosci Res. 2005 Feb 15;79(4):476-87
references.pmid: 15635609
references.citation: J Neurol Sci. 2012 Jan 15;312(1-2):73-8
references.pmid: 21875721
references.citation: Behav Brain Res. 2006 Dec 15;175(2):362-73
references.pmid: 17067689
references.citation: J Comp Neurol. 1990 Aug 15;298(3):362-72
references.pmid: 2212109
references.citation: Brain Res. 1977 Jan 1;119(1):1-20
references.pmid: 63306
references.citation: J Neurol Sci. 1995 Dec;134 Suppl:57-68
references.pmid: 8847546
references.citation: Brain Res. 1982 May 6;239(1):57-69
references.pmid: 7093691
references.citation: Exp Neurol. 1993 Jan;119(1):128-39
references.pmid: 8432346
references.citation: Neurochem Res. 2001 Nov;26(11):1209-16
references.pmid: 11874202
references.citation: Crit Rev Neurobiol. 2006;18(1-2):145-57
references.pmid: 17725517
references.citation: Neuroscience. 1986 Feb;17(2):439-51
references.pmid: 3517690
references.citation: J Comp Neurol. 1979 Aug 15;186(4):621-55
references.pmid: 15116692
references.citation: Neuroscience. 1990;35(2):289-99
references.pmid: 2381510
references.citation: Neurosci Res. 1995 Jan;21(3):199-209
references.pmid: 7753501
references.citation: J Neurotrauma. 2002 Jan;19(1):85-98
references.pmid: 11852981
references.citation: J Neurosci. 2000 Dec 15;20(24):9252-63
references.pmid: 11125003
references.citation: Trends Neurosci. 1996 Aug;19(8):312-8
references.pmid: 8843599
references.citation: Brain Res Dev Brain Res. 1995 Feb 16;84(2):245-52
references.pmid: 7743644
references.citation: Glia. 1996 Dec;18(4):319-31
references.pmid: 8972800
references.citation: Arch Pathol. 1969 Mar;87(3):315-7
references.pmid: 5766257
references.citation: Int J Dev Neurosci. 2011 Oct;29(6):645-54
references.pmid: 21382467
references.citation: Brain Res. 1998 Oct 5;807(1-2):47-60
references.pmid: 9756993
references.citation: Neuroreport. 1997 Mar 3;8(4):947-50
references.pmid: 9141070
references.citation: Brain Res. 2009 Feb 13;1254:128-37
references.pmid: 19103181
references.citation: Science. 1978 Jul 14;201(4351):160-3
references.pmid: 663646
references.citation: Neurosci Biobehav Rev. 1997 Mar;21(2):143-50
references.pmid: 9062937
references.citation: Prog Neurobiol. 1998 Oct;56(2):149-71
references.pmid: 9760699
references.citation: Neurochem Res. 2006 Jul;31(7):957-65
references.pmid: 16841189
references.citation: Exp Neurol. 2007 Jul;206(1):114-25
references.pmid: 17531224
references.citation: Neuropathol Appl Neurobiol. 1995 Aug;21(4):277-89
references.pmid: 7494596
references.citation: Neurology. 1987 Aug;37(8):1281-6
references.pmid: 3614648
references.citation: Stroke. 1993 Dec;24(12 Suppl):I84-90
references.pmid: 8249026
references.citation: Neurochem Res. 2012 Apr;37(4):826-34
references.pmid: 22219128
references.citation: J Cereb Blood Flow Metab. 1990 Nov;10(6):850-9
references.pmid: 2211878
references.citation: Brain. 1966 Jun;89(2):317-48
references.pmid: 5939044
references.citation: Brain Res. 2008 Mar 14;1199:159-66
references.pmid: 18269931
references.citation: Exp Neurol. 1972 Sep;36(3):472-87
references.pmid: 4116095
references.citation: CNS Neurol Disord Drug Targets. 2013 Aug;12(5):698-714
references.pmid: 23469845
references.citation: Exp Brain Res. 1973 Jul 30;17(5):447-62
references.pmid: 4741248
references.citation: J Neurosci. 1991 Jul;11(7):2187-98
references.pmid: 1906091
references.citation: Neurochem Res. 2007 Nov;32(11):1927-31
references.pmid: 17564835
references.citation: J Cereb Blood Flow Metab. 1995 Nov;15(6):969-79
references.pmid: 7593358
references.citation: Brain Res. 2012 Feb 9;1437:50-7
references.pmid: 22230668
references.citation: Int J Dev Neurosci. 1993 Apr;11(2):239-47
references.pmid: 8328304
references.citation: Brain Res. 1998 May 4;792(1):59-65
references.pmid: 9593822
references.citation: J Comp Neurol. 1984 Jan 1;222(1):69-80
references.pmid: 6365983
references.citation: Exp Neurol. 1974 Dec;45(3):503-8
references.pmid: 4435072
references.citation: Epilepsy Res Suppl. 1992;7:197-209
references.pmid: 1334663
references.citation: Neurosci Res. 2003 Oct;47(2):245-53
references.pmid: 14512150
references.citation: Acta Neuropathol. 1984;62(3):201-8
references.pmid: 6695554
references.citation: Neurosci Res. 2007 Dec;59(4):457-66
references.pmid: 17920148
references.citation: Brain Res. 1990 Mar 26;512(1):7-14
references.pmid: 2337809
references.citation: Trends Neurosci. 2006 Feb;29(2):68-74
references.pmid: 16406093
references.citation: Brain Res Brain Res Rev. 1997 Sep 19;24(2-3):115-95
references.pmid: 9385454
references.citation: Acta Neuropathol. 1992;83(5):504-9
references.pmid: 1621506
references.citation: Brain Res. 2000 Aug 25;874(2):123-30
references.pmid: 10960596
references.citation: Neuroscience. 1988 Nov;27(2):387-402
references.pmid: 2464145
references.citation: Nature. 1988 Nov 10;336(6195):170-3
references.pmid: 3185735
references.citation: Neurosci Lett. 2005 Jan 10;373(2):159-64
references.pmid: 15567573
references.citation: Acta Neuropathol. 1992;85(1):79-87
references.pmid: 1285498