title: IL-4 polymorphisms, HRCT score and lung tissue markers in idiopathic pulmonary fibrosis.
abstract: OBJECTIVE
  We studied the influence of IL-4 gene polymorphisms on the IPF phenotype, i.e., extent of radiological changes (HRCT interstitial (IS) and alveolar (AS) score) and histopathological markers from lung biopsies.
  
  
  METHODS
  46 IPF patients underwent genotyping, 43 of them had HRCT and 14 patients had a surgical lung biopsy. The HRCT scans were evaluated for AS and IS. The histopathological evaluation comprised myofibroblast foci (MF), intensity of inflammation and fibrosis (Ashcroft score) and numbers of eosinophils and granulomas. For immunohistochemical evaluation primary antibodies against PAR-2, CD124, TGF beta, YY-1 and TSLP were used. The IL-4 and IL-4 R alpha gene polymorphisms were characterized.
  
  
  RESULTS
  We found a correlation between eosinophils in lung biopsies and AS. The Ashcroft score was higher in IL-4 HA 2 GCC and MF were more frequent in IL-4 HA 2 TCC carriers. A relationship was found between IL-4 (-1098) A2 T and PAR-2 expression and IL-4 (-590) A1 T, IL-4 HA1TTT and CD124 expression. AS was lower in IL-4 (-590) A1 C, in IL-4 HA1 TCC and in IL-4RA (+1902) A1 A carriers.
  
  
  CONCLUSIONS
  We suggest that the polymorphisms of IL-4 genes might influence the phenotype of IPF reflected by histopathological changes in lung biopsies and HRCT score.
authors.affiliation: Department of Respiratory Medicine, Thomayer Hospital, Prague, Czech Republic. Electronic address: tichadohoda@volny.cz.
authors.forename: Martina
authors.initials: M
authors.lastname: Vasakova
authors.forename: Martina
authors.initials: M
authors.lastname: Sterclova
authors.forename: Radoslav
authors.initials: R
authors.lastname: Matej
authors.forename: Tomas
authors.initials: T
authors.lastname: Olejar
authors.forename: Libor
authors.initials: L
authors.lastname: Kolesar
authors.forename: Jelena
authors.initials: J
authors.lastname: Skibova
authors.forename: Ilja
authors.initials: I
authors.lastname: Striz
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000483:Alleles; D015415:Biomarkers; D001706:Biopsy; D004804:Eosinophils / Q000378:metabolism; D005260:Female; D005838:Genotype; D006801:Humans; D054990:Idiopathic Pulmonary Fibrosis / Q000175:diagnosis* / Q000235:genetics*; D015847:Interleukin-4 / Q000235:genetics*; D053662:Interleukin-4 Receptor alpha Subunit / Q000235:genetics / Q000378:metabolism; D007958:Leukocyte Count; D008168:Lung / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D008264:Macrophages / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D010641:Phenotype; D011110:Polymorphism, Genetic
chemical_list: D015415:Biomarkers; D053662:Interleukin-4 Receptor alpha Subunit; D015847:Interleukin-4