title: Identification of the set of genes, including nonannotated morA, under the direct control of ModE in Escherichia coli.
abstract: ModE is the molybdate-sensing transcription regulator that controls the expression of genes related to molybdate homeostasis in Escherichia coli. ModE is activated by binding molybdate and acts as both an activator and a repressor. By genomic systematic evolution of ligands by exponential enrichment (SELEX) screening and promoter reporter assays, we have identified a total of nine operons, including the hitherto identified modA, moaA, dmsA, and napF operons, of which six were activated by ModE and three were repressed. In addition, two promoters were newly identified and direct transcription of novel genes, referred to as morA and morB, located on antisense strands of yghW and torY, respectively. The morA gene encodes a short peptide, MorA, with an unusual initiation codon. Surprisingly, overexpression of the morA 5' untranslated region exhibited an inhibitory influence on colony formation of E. coli K-12.
authors.affiliation: Department of Frontier Bioscience.
authors.forename: Tatsuaki
authors.initials: T
authors.lastname: Kurata
authors.forename: Akira
authors.initials: A
authors.lastname: Katayama
authors.forename: Masakazu
authors.initials: M
authors.lastname: Hiramatsu
authors.forename: Yuya
authors.initials: Y
authors.lastname: Kiguchi
authors.forename: Masamitsu
authors.initials: M
authors.lastname: Takeuchi
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Watanabe
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ogasawara
authors.forename: Akira
authors.initials: A
authors.lastname: Ishihama
authors.forename: Kaneyoshi
authors.initials: K
authors.lastname: Yamamoto
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology*; D016680:Genome, Bacterial; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D018085:Regulon; D014157:Transcription Factors / Q000378:metabolism*
chemical_list: D029968:Escherichia coli Proteins; C491064:ModE protein, E coli; C584321:MorA protein, E coli; D014157:Transcription Factors
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