title: Treatment of high-salinity chemical wastewater by indigenous bacteria--bioaugmented contact oxidation.
abstract: A 90 m(3) biological contact oxidation system in chemical factory was bioaugmented with three strains of indigenous salt-tolerant bacteria. These three strains were screened from contaminative soil in situ. Their activity of growth and degradation was investigated with lab-scale experiments. Their salt-tolerant mechanism was confirmed to be compatible-solutes strategy for moderately halophilic bacteria, with amino acid and betaine playing important roles. The running conditions of the system were recorded for 150 days. The indigenous bacteria had such high suitability that the reactor got steady rapidly and the removal of COD maintained above 90%. It was introduced that biofilm fragments in sedimentation tank were inversely flowed to each reaction tank, and quantitative PCR demonstrated that this process could successfully maintain the bacterial abundance in the reaction tanks. In addition, the T-RFLP revealed that bioaugmented strains dominated over others in the biofilm.
authors.affiliation: Key Laboratory of Molecular Microbiology and Technology for Ministry of Education, Nankai University, Tianjin 300071, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Li
authors.forename: Mengdi
authors.initials: M
authors.lastname: Wang
authors.forename: Jun
authors.initials: J
authors.lastname: Feng
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Wang
authors.forename: Yanyan
authors.initials: Y
authors.lastname: Gu
authors.forename: Cunjiang
authors.initials: C
authors.lastname: Song
authors.forename: Shufang
authors.initials: S
authors.lastname: Wang
mesh_terms: D001419:Bacteria / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism*; D001673:Biodegradation, Environmental; D018441:Biofilms; D057919:Biological Oxygen Demand Analysis; D019149:Bioreactors / Q000382:microbiology; D007220:Industrial Waste / Q000032:analysis*; D009584:Nitrogen / Q000032:analysis; D010084:Oxidation-Reduction; D010758:Phosphorus / Q000032:analysis; D010802:Phylogeny; D012150:Polymorphism, Restriction Fragment Length; D054712:Salinity; D055049:Salt Tolerance; D014865:Waste Disposal, Fluid; D062065:Wastewater / Q000382:microbiology*; D018508:Water Purification / Q000379:methods*
chemical_list: D007220:Industrial Waste; D009584:Nitrogen; D010758:Phosphorus; D062065:Wastewater
keywords: Bacterial community and abundance; Bioaugmented contact oxidation; High-salinity chemical wastewater; Indigenous bacteria; Salt-tolerant mechanism