title: DmGTSF1 is necessary for Piwi-piRISC-mediated transcriptional transposon silencing in the Drosophila ovary.
abstract: The Piwi-piRNA (PIWI-interacting RNA) complex (Piwi-piRISC) in Drosophila ovarian somatic cells represses transposons transcriptionally to maintain genome integrity; however, the underlying mechanisms remain obscure. Here, we reveal that DmGTSF1, a Drosophila homolog of gametocyte-specific factor 1 (GTSF1) (which is required for transposon silencing in mouse testes), is necessary for Piwi-piRISC to repress target transposons and neighboring genes. DmGTSF1 depletion affected neither piRNA biogenesis nor nuclear import of Piwi-piRISC. DmGTSF1 mutations caused derepression of transposons and loss of ovary follicle layers, resulting in female infertility. We suggest that DmGTSF1, a nuclear Piwi interactor, is an integral factor in Piwi-piRISC-mediated transcriptional silencing.
authors.affiliation: Department of Molecular Biology, Keio University School of Medicine, Tokyo 160-8582, Japan
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Ohtani
authors.forename: Yuka W
authors.initials: YW
authors.lastname: Iwasaki
authors.forename: Aoi
authors.initials: A
authors.lastname: Shibuya
authors.forename: Haruhiko
authors.initials: H
authors.lastname: Siomi
authors.forename: Mikiko C
authors.initials: MC
authors.lastname: Siomi
authors.forename: Kuniaki
authors.initials: K
authors.lastname: Saito
mesh_terms: D000818:Animals; D060565:Argonaute Proteins / Q000235:genetics / Q000378:metabolism*; D004251:DNA Transposable Elements / Q000235:genetics*; D029721:Drosophila Proteins / Q000235:genetics* / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000235:genetics* / Q000378:metabolism*; D005260:Female; D018507:Gene Expression Regulation, Developmental; D020868:Gene Silencing; D009154:Mutation; D009687:Nuclear Proteins / Q000235:genetics* / Q000378:metabolism*; D010053:Ovary / Q000378:metabolism; D034741:RNA, Small Interfering / Q000378:metabolism*
chemical_list: D060565:Argonaute Proteins; D004251:DNA Transposable Elements; D029721:Drosophila Proteins; D009687:Nuclear Proteins; D034741:RNA, Small Interfering; C584031:arx protein, Drosophila; C116498:piwi protein, Drosophila
keywords: DmGTSF1; Drosophila; Piwi; RNA silencing; piRNA; transposon
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