title: Cross-linking and mass spectrometry methodologies to facilitate structural biology: finding a path through the maze.
abstract: Multiprotein complexes, rather than individual proteins, make up a large part of the biological macromolecular machinery of a cell. Understanding the structure and organization of these complexes is critical to understanding cellular function. Chemical cross-linking coupled with mass spectrometry is emerging as a complementary technique to traditional structural biology methods and can provide low-resolution structural information for a multitude of purposes, such as distance constraints in computational modeling of protein complexes. In this review, we discuss the experimental considerations for successful application of chemical cross-linking-mass spectrometry in biological studies and highlight three examples of such studies from the recent literature. These examples (as well as many others) illustrate the utility of a chemical cross-linking-mass spectrometry approach in facilitating structural analysis of large and challenging complexes.
authors.affiliation: MS K8-98, Biological Sciences Division, Pacific Northwest National Laboratory, Richland, WA 99352, USA. Eric.Merkley@pnnl.gov
authors.forename: Eric D
authors.initials: ED
authors.lastname: Merkley
authors.forename: John R
authors.initials: JR
authors.lastname: Cort
authors.forename: Joshua N
authors.initials: JN
authors.lastname: Adkins
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D036022:Capsid Proteins / Q000737:chemistry; D019295:Computational Biology; D003432:Cross-Linking Reagents / Q000737:chemistry; D047908:Intracellular Signaling Peptides and Proteins / Q000737:chemistry; D052679:Luteoviridae / Q000737:chemistry; D013058:Mass Spectrometry / Q000379:methods*; D008958:Models, Molecular; D018832:Molecular Chaperones; D046912:Multiprotein Complexes / Q000032:analysis / Q000737:chemistry*; D054648:Protein Phosphatase 2 / Q000737:chemistry
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D036022:Capsid Proteins; D003432:Cross-Linking Reagents; C490467:IGBP1 protein, human; D047908:Intracellular Signaling Peptides and Proteins; D018832:Molecular Chaperones; D046912:Multiprotein Complexes; D054648:Protein Phosphatase 2
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