title: Decline of lactate in tumor tissue after ketogenic diet: in vivo microdialysis study in patients with head and neck cancer.
abstract: In head and neck squamous cell carcinoma (HNSCC) aerobic glycolysis is the key feature for energy supply of the tumor. Quantitative microdialysis (μD) offers an online method to measure parameters of the carbohydrate metabolism in vivo. The aim was to standardize a quantitative μD-study in patients with HNSCC and to prove if a ketogenic diet would differently influence the carbohydrate metabolism of the tumor tissue. Commercially available 100 kDa-CMA71-μD- catheters were implanted in tumor-free and in tumor tissue in patients with HNSCC for simultaneous measurements up to 5 days. The metabolic pattern and circadian rhythm of urea, glucose, lactate, and pyruvate was monitored during 24 h of western diet and subsequent up to 4 days of ketogenic diet. After 3 days of ketogenic diet the mean lactate concentration declines to a greater extent in the tumor tissue than in the tumor-free mucosa, whereas the mean glucose and pyruvate concentrations rise. The in vivo glucose metabolism of the tumor tissue is clearly influenced by nutrition. The decline of mean lactate concentration in the tumor tissue after ketogenic diet supports the hypothesis that HNSCC tumor cells might use lactate as fuel for oxidative glucose metabolism.
authors.affiliation: Department of Ear, Nose and Throat, University Hospital Schleswig-Holstein, Luebeck, Germany. ursula.schroeder@uksh.de
authors.forename: U
authors.initials: U
authors.lastname: Schroeder
authors.forename: B
authors.initials: B
authors.lastname: Himpe
authors.forename: R
authors.initials: R
authors.lastname: Pries
authors.forename: R
authors.initials: R
authors.lastname: Vonthein
authors.forename: S
authors.initials: S
authors.lastname: Nitsch
authors.forename: B
authors.initials: B
authors.lastname: Wollenberg
mesh_terms: D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000178:diet therapy*; D002940:Circadian Rhythm; D055423:Diet, Ketogenic; D005260:Female; D005947:Glucose / Q000378:metabolism; D006258:Head and Neck Neoplasms / Q000178:diet therapy*; D006801:Humans; D019344:Lactic Acid / Q000378:metabolism*; D008297:Male; D017551:Microdialysis / Q000379:methods*; D008875:Middle Aged; D019289:Pyruvic Acid / Q000378:metabolism; D015203:Reproducibility of Results; D000077195:Squamous Cell Carcinoma of Head and Neck
chemical_list: D019344:Lactic Acid; D019289:Pyruvic Acid; D005947:Glucose