title: [Development and application of separation materials for mixed-mode chromatography].
abstract: Mixed-mode chromatography has received more and more attention due to its unique chromatographic characteristics recently. In this review, a summary of the development and applications of mixed-mode chromatography is presented. According to the types of hydrophilic interaction/ion-exchange mixed-mode chromatography (HILIC/IEX), reversed-phase/hydrophilic interaction mixed-mode chromatography (RPLC/HILIC) and reversed-phase/ion-exchange mixed-mode chromatography (RPLC/IEX), the preparation and applications of each type are introduced. Compared with single mode chromatography, the selectivity as well as the sample loading capacity of the mixed-mode chromatography can be improved. The researches mostly focus on the design of mixed-mode stationary phases and the applications especially to bio-analysis. The mixed-mode chromatography will be a potent approach for the analysis and separation of complex samples, and it will also be an effective and complementary tool in practical separation work.
authors.affiliation: Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, China.
authors.forename: Xuefang
authors.initials: X
authors.lastname: Dong
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Cai
authors.forename: Aijin
authors.initials: A
authors.lastname: Shen
authors.forename: Jie
authors.initials: J
authors.lastname: Wei
authors.forename: Zhimou
authors.initials: Z
authors.lastname: Guo
authors.forename: Xinmiao
authors.initials: X
authors.lastname: Liang