title: [Increased survival of free fat grafts with adipose-derived stem cells and platelet-rich plasma in nude mice].
abstract: OBJECTIVE
  To investigate the impact of combined adipose-derived stem cells (ASCs) and platelet-rich plasma (PRP) on the survival of transplanted fat.
  
  
  METHODS
  The ASCs were isolated and cultured from fat tissues by enzyme digestion; and the PRP was prepared by two-step ultracentrifugation. The grafts of fat granules were divided into test groups (ASCs + PRP + fat granules, PRP + fat granules, ASCs + fat granules) and control groups (PBS + fat granules). The grafts were injected into the left and right dorsal subcutaneous areas of nude mice. General observations, volume measurements and microscope examinations were conducted 10 d, 30 d, 60 d and 90 d after transplantation.
  
  
  RESULTS
  The grafts of the mice in the ASCs + PRP group showed soft structure, with light yellow color and closer to normal adipose tissue compared with those in other groups. Greater survival volumes (P < 0.05) and better histology were also observed in the grafts of the mice in the ASCs + PRP group.
  
  
  CONCLUSIONS
  The fat grafts consisting of PRP and ASCs constitute an ideal transplant strategy, which could provide a valuable and needed tool in plastic and reconstructive surgery.
authors.affiliation: State Key Laboratory of Oral Diseases, Sichuan University, Chengdu 610041, China.
authors.forename: Kun
authors.initials: K
authors.lastname: Li
authors.forename: Feng
authors.initials: F
authors.lastname: Li
authors.forename: Li-Juan
authors.initials: LJ
authors.lastname: Guo
authors.forename: Jie
authors.initials: J
authors.lastname: Li
authors.forename: Ke
authors.initials: K
authors.lastname: Huang
authors.forename: Wei-Hua
authors.initials: WH
authors.lastname: Guo
authors.forename: Wei-Dong
authors.initials: WD
authors.lastname: Tian
mesh_terms: D017667:Adipocytes / Q000166:cytology; D000273:Adipose Tissue / Q000166:cytology / Q000637:transplantation*; D000818:Animals; D005260:Female; D006085:Graft Survival; D008297:Male; D051379:Mice; D008819:Mice, Nude; D053657:Platelet-Rich Plasma; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000166:cytology; D023822:Tissue Engineering; D016378:Tissue Transplantation / Q000379:methods; D014183:Transplantation, Heterologous