title: Dynamin phosphorylation controls optimization of endocytosis for brief action potential bursts.
abstract: Modulation of synaptic vesicle retrieval is considered to be potentially important in steady-state synaptic performance. Here we show that at physiological temperature endocytosis kinetics at hippocampal and cortical nerve terminals show a bi-phasic dependence on electrical activity. Endocytosis accelerates for the first 15-25 APs during bursts of action potential firing, after which it slows with increasing burst length creating an optimum stimulus for this kinetic parameter. We show that activity-dependent acceleration is only prominent at physiological temperature and that the mechanism of this modulation is based on the dephosphorylation of dynamin 1. Nerve terminals in which dynamin 1 and 3 have been replaced with dynamin 1 harboring dephospho- or phospho-mimetic mutations in the proline-rich domain eliminate the acceleration phase by either setting endocytosis at an accelerated state or a decelerated state, respectively. DOI:http://dx.doi.org/10.7554/eLife.00845.001.
authors.affiliation: Department of Biochemistry , Weill Cornell Medical College , New York , United States ; The David Rockefeller Graduate Program , Rockefeller University , New York , United States.
authors.forename: Moritz
authors.initials: M
authors.lastname: Armbruster
authors.forename: Mirko
authors.initials: M
authors.lastname: Messa
authors.forename: Shawn M
authors.initials: SM
authors.lastname: Ferguson
authors.forename: Pietro
authors.initials: P
authors.lastname: De Camilli
authors.forename: Timothy A
authors.initials: TA
authors.lastname: Ryan
mesh_terms: D000200:Action Potentials; D002118:Calcium / Q000378:metabolism; D034281:Dynamins / Q000378:metabolism*; D004705:Endocytosis; D006624:Hippocampus / Q000378:metabolism / Q000502:physiology; D006801:Humans; D007700:Kinetics; D010766:Phosphorylation; D013696:Temperature
chemical_list: D034281:Dynamins; D002118:Calcium
keywords: Mouse; Rat; dynamin; endocytosis; phosphorylation; synaptic vesicle
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