title: Subcellular protein localization (cell envelope) in Phaeobacter inhibens DSM 17395.
abstract: Phaeobacter inhibens DSM 17395 is a metabolically versatile, secondary metabolite producing and surface colonizing member of the alphaproteobacterial Roseobacter clade. Proteins compartmentalized across the Gram-negative cell envelope are expected to be relevant for the habitat success of P. inhibens DSM 17395. Subcellular fractionation was followed by gel- or nano-LC-based separation of proteins and peptides, respectively. Subsequent MS-based identification of in total 1187 proteins allowed allocation to cytoplasm (303 proteins), cytoplasmic membrane (346), periplasm (325), outer membrane (76), and extracellular milieu (22). Multidimensional scaling was used to visualize the spreading of heuristically allocated proteins across the five different compartments. Experimentally inferred subcellular protein localization was compared with PSORTb prediction of protein secretion and membrane localization. Determined subcellular localizations of identified proteins were interpreted to reconstruct the functional traits of the different cell envelope compartments, in particular protein secretion and sorting, direct effector molecule transit, and cell envelope biogenesis. From a proteogenomic perspective, functional prediction of 74 genes (including 17 coding for proteins of hitherto unknown function) could be refined.
authors.affiliation: Institute for Chemistry and Biology of the Marine Environment (ICBM), Carl von Ossietzky University Oldenburg, Oldenburg, Germany.
authors.forename: Sebastian
authors.initials: S
authors.lastname: Koßmehl
authors.forename: Lars
authors.initials: L
authors.lastname: Wöhlbrand
authors.forename: Katharina
authors.initials: K
authors.lastname: Drüppel
authors.forename: Christoph
authors.initials: C
authors.lastname: Feenders
authors.forename: Bernd
authors.initials: B
authors.lastname: Blasius
authors.forename: Ralf
authors.initials: R
authors.lastname: Rabus
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism*; D002473:Cell Wall / Q000378:metabolism*; D030562:Databases, Protein; D004591:Electrophoresis, Polyacrylamide Gel; D021381:Protein Transport; D020543:Proteome / Q000378:metabolism; D040901:Proteomics; D041904:Roseobacter / Q000378:metabolism*; D013347:Subcellular Fractions / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D020543:Proteome
keywords: Cell envelope; Membrane proteomics; Microbiology; Multidimensional scaling; PSORTb; Subcellular fractionation