title: Progesterone reverts LPS-reduced FAAH activity in murine peripheral blood mononuclear cells by a receptor-mediated fashion.
abstract: Increased anandamide concentrations are associated with pregnancy failure. Anandamide levels are regulated by the fatty acid amide hydrolase (FAAH). The aim of the study was to investigate the role of progesterone (P) on FAAH modulation in murine peripheral blood mononuclear cells (PBMC) under septic conditions. We observed that in vivo administration of LPS to non-pregnant (NP) mice decreased FAAH activity of PBMC while in pregnant mice no changes in FAAH activity were observed. NP animals administered with P had a similar response to LPS as the pregnant animals. Also, NP mice injected with P antagonist and P showed that the effect of P on LPS-reduced FAAH activity was impaired. Furthermore, LPS produced a decrease in the ratio of PR-B/PR-A in NP animals. Our results showed that, in our model the endotoxin decreased PBMC's FAAH activity and this condition was reverted by P in a receptor-mediated fashion.
authors.affiliation: Laboratory of Physiopathology of Pregnancy and Labor, Center for Pharmacological and Botanical Studies, National Research Council, School of Medicine, University of Buenos Aires, Argentina. Electronic address: manuwolfson@gmail.com.
authors.forename: Manuel L
authors.initials: ML
authors.lastname: Wolfson
authors.forename: Julieta
authors.initials: J
authors.lastname: Aisemberg
authors.forename: Ana I
authors.initials: AI
authors.lastname: Salazar
authors.forename: Ana P
authors.initials: AP
authors.lastname: Domínguez Rubio
authors.forename: Claudia A
authors.initials: CA
authors.lastname: Vercelli
authors.forename: Ana M
authors.initials: AM
authors.lastname: Franchi
mesh_terms: D000581:Amidohydrolases / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D005260:Female; D015870:Gene Expression; D007963:Leukocytes, Mononuclear / Q000201:enzymology / Q000276:immunology; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D011247:Pregnancy; D011374:Progesterone / Q000502:physiology*; D011980:Receptors, Progesterone / Q000235:genetics / Q000378:metabolism; D013601:T-Lymphocytes / Q000201:enzymology* / Q000276:immunology; D000099252:Fatty Acid Amide Hydrolases
chemical_list: D000581:Amidohydrolases; D008070:Lipopolysaccharides; D011374:Progesterone; D011980:Receptors, Progesterone; D000099252:Fatty Acid Amide Hydrolases
keywords: 2-AG; 2-arachidonoylglycerol; AEA; CB; Fatty acid amide hydrolase; LONA; LPS; Lipopolysaccharide; Lonaprisan; MAGL; Mouse; N-arachidonoyl phosphatidylethanolamine; NAPE-PLD; P; PBMC; PR; Peripheral blood mononuclear cells; Pregnant; Progesterone; anandamide; cannabinoid receptor EmRe: embryonic resorption FAAH: fatty acid amide hydrolase; lipopolysacharide; monoacylglycerol lipase; peripheral blood mononuclear cells; progesterone; progesterone receptor