title: The peptidoglycan hydrolase of Staphylococcus aureus bacteriophage 11 plays a structural role in the viral particle.
abstract: The role of virion-associated peptidoglycan hydrolases (VAPGHs) in the phage infection cycle is not clear. gp49, the VAPGH from Staphylococcus aureus phage 11, is not essential for phage growth but stabilizes the viral particles. 11Δ49 phages showed a reduced burst size and delayed host lysis. Complementation of gp49 with HydH5 from bacteriophage vB_SauS-phiIPLA88 restored the wild-type phenotype.
authors.affiliation: DairySafe Group, Department of Technology and Biotechnology of Dairy Products, Instituto de Productos Lácteos de Asturias (IPLA-CSIC), Villaviciosa, Asturias, Spain.
authors.forename: Lorena
authors.initials: L
authors.lastname: Rodríguez-Rubio
authors.forename: Nuria
authors.initials: N
authors.lastname: Quiles-Puchalt
authors.forename: Beatriz
authors.initials: B
authors.lastname: Martínez
authors.forename: Ana
authors.initials: A
authors.lastname: Rodríguez
authors.forename: José R
authors.initials: JR
authors.lastname: Penadés
authors.forename: Pilar
authors.initials: P
authors.lastname: García
mesh_terms: D001433:Bacteriolysis; D017353:Gene Deletion; D005816:Genetic Complementation Test; D009238:N-Acetylmuramoyl-L-alanine Amidase / Q000235:genetics / Q000378:metabolism*; D013204:Staphylococcus Phages / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development*; D013211:Staphylococcus aureus / Q000821:virology*; D015678:Viral Structural Proteins / Q000235:genetics / Q000378:metabolism*; D014771:Virion / Q000235:genetics / Q000378:metabolism*
chemical_list: D015678:Viral Structural Proteins; D009238:N-Acetylmuramoyl-L-alanine Amidase
references.citation: Appl Environ Microbiol. 2004 Oct;70(10):6076-85
references.pmid: 15466553
references.citation: Mol Microbiol. 2000 Jul;37(2):345-55
references.pmid: 10931329
references.citation: Mol Microbiol. 2006 Dec;62(6):1569-85
references.pmid: 17083467
references.citation: Virology. 1967 Sep;33(1):155-66
references.pmid: 4227577
references.citation: Bacteriophage. 2011 Jan;1(1):46-49
references.pmid: 21687534
references.citation: Gene. 2002 May 1;289(1-2):109-18
references.pmid: 12036589
references.citation: Comput Biol Chem. 2008 Oct;32(5):367-9
references.pmid: 18479969
references.citation: J Biol Chem. 2013 Feb 22;288(8):5581-90
references.pmid: 23300085
references.citation: Mol Microbiol. 2005 May;56(3):836-44
references.pmid: 15819636
references.citation: J Mol Biol. 2005 Mar 4;346(4):1013-20
references.pmid: 15701513
references.citation: Int J Food Microbiol. 2003 Sep 15;86(3):213-22
references.pmid: 12915032
references.citation: J Biol Chem. 2008 May 16;283(20):13556-64
references.pmid: 18348984
references.citation: Nature. 1983 Oct 20-26;305(5936):709-12
references.pmid: 6226876
references.citation: Virol J. 2010 Dec 03;7:355
references.pmid: 21129200
references.citation: J Bacteriol. 2004 Jun;186(11):3480-91
references.pmid: 15150235
references.citation: Appl Microbiol Biotechnol. 2011 Oct;92(1):1-11
references.pmid: 21796380
references.citation: J Bacteriol. 1985 Jan;161(1):238-42
references.pmid: 3881387
references.citation: Crit Rev Microbiol. 2013 Nov;39(4):427-34
references.pmid: 22991936
references.citation: J Theor Biol. 2007 Dec 7;249(3):582-92
references.pmid: 17919662
references.citation: Mol Microbiol. 2000 Jul;37(2):356-63
references.pmid: 10931330
references.citation: Structure. 2012 Feb 8;20(2):326-39
references.pmid: 22325780
references.citation: Mol Microbiol. 2004 Feb;51(4):1169-83
references.pmid: 14763988
references.citation: BMC Microbiol. 2011 Aug 09;11:181
references.pmid: 21827665
references.citation: Appl Environ Microbiol. 2009 Dec;75(24):7663-73
references.pmid: 19837832
references.citation: BMC Microbiol. 2011 Jun 17;11:138
references.pmid: 21682850