title: Fed-batch semi-simultaneous saccharification and fermentation of reed pretreated with liquid hot water for bio-ethanol production using Saccharomyces cerevisiae.
abstract: Reed was pretreated with liquid hot water (LHW) and then subjected to fed-batch semi-simultaneous saccharification and fermentation (S-SSF) to obtain high ethanol concentration and yield. Results show that water-insoluble solid (WIS) produced from reed pretreated at 180 and 210°C could be effectively converted to ethanol by using Saccharomyces cerevisiae. The optimum conditions for bio-ethanol production are as follows: fermentation temperature of 36°C, pH of 4.8 with cellulase loading of 40 filter paper activity units/g oven-dried WIS, and 18 h pre-hydrolysis at 50°C. Approximately 6.4% (w/v) fed-batch substrate was added after 6 h of the 18 h enzymatic pre-hydrolysis. The highest ethanol concentration of 39.4 g/L was achieved. The conversion of glucan in the WIS to ethanol reached 79.1% (180°C) and 75.1% (210°C) respectively. The ethanol yields per kg of oven-dried reed were 283 g/L at 180°C and 244 g/L at 210°C.
authors.affiliation: Dalian Polytechnic University, Dalian 116034, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Lu
authors.forename: Xuezhi
authors.initials: X
authors.lastname: Li
authors.forename: Ruifeng
authors.initials: R
authors.lastname: Yang
authors.forename: Lei
authors.initials: L
authors.lastname: Yang
authors.forename: Jian
authors.initials: J
authors.lastname: Zhao
authors.forename: Yanjun
authors.initials: Y
authors.lastname: Liu
authors.forename: Yinbo
authors.initials: Y
authors.lastname: Qu
mesh_terms: D061249:Batch Cell Culture Techniques / Q000379:methods*; D056804:Biofuels; D050260:Carbohydrate Metabolism / Q000187:drug effects*; D002480:Cellulase / Q000378:metabolism; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation / Q000187:drug effects*; D005947:Glucose / Q000378:metabolism; D006358:Hot Temperature; D006868:Hydrolysis; D006109:Poaceae / Q000187:drug effects / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000378:metabolism*; D012995:Solubility; D013379:Substrate Specificity; D013997:Time Factors; D014867:Water / Q000494:pharmacology*
chemical_list: D056804:Biofuels; D014867:Water; D000431:Ethanol; D002480:Cellulase; D005947:Glucose
keywords: Enzymatic hydrolysis; Ethanol; Liquid hot water pretreatment; Reed; Semi-simultaneous saccharification and fermentation