title: Advances in kinetic isotope effect measurement techniques for enzyme mechanism study.
abstract: Kinetic isotope effects (KIEs) are a very powerful tool for investigating enzyme mechanisms. Precision of measurement is the most important factor for KIE determinations, especially for small heavy atom KIEs. Internal competition is commonly used to measure small KIEs on V/K. Several methods, including such as liquid scintillation counting, mass spectrometry, nuclear magnetic resonance spectroscopy and polarimetry have been used to determine KIEs. In this paper, which does not aspire to be an exhaustive review, we briefly review different experimental approaches for the measurement of KIEs on enzymatic reaction with an emphasis on newer techniques employing mass spectrometry and nuclear magnetic resonance spectrometry as well as some corresponding examples.
authors.affiliation: Department of Biochemistry, Case Western Reserve University School of Medicine, 10900 Euclid Ave., Cleveland, OH 44106, USA. hxg95@case.edu
authors.forename: Hong
authors.initials: H
authors.lastname: Gu
authors.forename: Shuming
authors.initials: S
authors.lastname: Zhang
mesh_terms: D004798:Enzymes / Q000737:chemistry*; D007554:Isotopes / Q000737:chemistry*; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D013058:Mass Spectrometry / Q000379:methods*; D012588:Scintillation Counting
chemical_list: D004798:Enzymes; D007554:Isotopes
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