title: Escherichia coli F1Fo-ATP synthase with a b/δ fusion protein allows analysis of the function of the individual b subunits.
abstract: The "stator stalk" of F1Fo-ATP synthase is essential for rotational catalysis as it connects the nonrotating portions of the enzyme. In Escherichia coli, the stator stalk consists of two (identical) b subunits and the δ subunit. In mycobacteria, one of the b subunits and the δ subunit are replaced by a b/δ fusion protein; the remaining b subunit is of the shorter b' type. In the present study, it is shown that it is possible to generate a functional E. coli ATP synthase containing a b/δ fusion protein. This construct allowed the analysis of the roles of the individual b subunits. The full-length b subunit (which in this case is covalently linked to δ in the fusion protein) is responsible for connecting the stalk to the catalytic F1 subcomplex. It is not required for interaction with the membrane-embedded Fo subcomplex, as its transmembrane helix can be removed. Attachment to Fo is the function of the other b subunit which in turn has only a minor (if any at all) role in binding to δ. Also in E. coli the second b subunit can be shortened to a b' type.
authors.affiliation: From the Department of Chemistry and Biochemistry and the Center for Chemical Biology, Texas Tech University, Lubbock, Texas 79409 and the Center for Membrane Protein Research, Texas Tech University Health Sciences Center, Lubbock, Texas 79430.
authors.forename: Chathurada S
authors.initials: CS
authors.lastname: Gajadeera
authors.forename: Joachim
authors.initials: J
authors.lastname: Weber
mesh_terms: D025243:Bacterial Proton-Translocating ATPases / Q000378:metabolism*; D002384:Catalysis; D002462:Cell Membrane / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology*; D029968:Escherichia coli Proteins / Q000378:metabolism*; D010957:Plasmids / Q000378:metabolism; D017354:Point Mutation; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D006180:Proton-Translocating ATPases / Q000737:chemistry*; D011993:Recombinant Fusion Proteins / Q000378:metabolism
chemical_list: D029968:Escherichia coli Proteins; D011993:Recombinant Fusion Proteins; D025243:Bacterial Proton-Translocating ATPases; C557221:Escherichia coli Proton-Translocating ATPase; D006180:Proton-Translocating ATPases
keywords: ATP Synthase; Enzyme Catalysis; Enzyme Mechanisms; Membrane Proteins; Protein Assembly
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