title: The changes in MUC5AC expression in gastric cancer before and after Helicobacter pylori eradication.
abstract: OBJECTIVE
  To investigate MUC5AC expression in gastric cancer before and after Hp eradication.
  
  
  METHODS
  The MUC5AC protein and mRNA were detected in gastric cancer tissue by western blot and real time PCR protocols before and after Hp eradication (Hp positive group). Gastric cancer tissue without Hp infection served as the control group (Hp negative group).
  
  
  RESULTS
  The MUC5AC protein and mRNA expression was more significantly increased in gastric cancer after Hp eradication as compared to that before Hp eradication, but it was significantly lower than of the control group. The relative amount of MUC5AC in the well-differentiated cancer was higher than that of the moderately or poorly-differentiated cancer, in either Hp positive or control groups. The relative amount of MUC5AC in cancer tissues with more than five metastatic lymph nodes was significantly lower than that of the cancer tissues with five or less metastatic lymph nodes, and was significantly lower in the Hp positive group as compared to that of the control group.
  
  
  CONCLUSIONS
  The reduction of the MUC5AC might be related to gastric carcinogenesis caused by Hp and the progression of gastric cancer.
authors.affiliation: Department of Gastroenterology, First People's Hospital of Yuhang District, Hangzhou 31100, Zhejiang Province, China. Electronic address: shidingyuhang@163.com.
authors.forename: Ding
authors.initials: D
authors.lastname: Shi
authors.affiliation: Department of Gastroenterology, First People's Hospital of Yuhang District, Hangzhou 31100, Zhejiang Province, China. Electronic address: Xinmin2005_q@sohu.com.
authors.forename: Xin-Min
authors.initials: XM
authors.lastname: Qiu
authors.affiliation: Department of General Surgery, Central Hospital of Yiwu city, Jinhua 32100, Zhejiang Province, China. Electronic address: Xiao_xiao_yu118@tom.com.
authors.forename: Xing-Jun
authors.initials: XJ
authors.lastname: Yan
mesh_terms: D000230:Adenocarcinoma / Q000378:metabolism* / Q000473:pathology; D000328:Adult; D000368:Aged; D015153:Blotting, Western; D016022:Case-Control Studies; D005260:Female; D016481:Helicobacter Infections / Q000188:drug therapy*; D016480:Helicobacter pylori; D006801:Humans; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D055271:Mucin 5AC / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction; D013274:Stomach Neoplasms / Q000378:metabolism* / Q000473:pathology
chemical_list: C524678:MUC5AC protein, human; D055271:Mucin 5AC; D012333:RNA, Messenger