title: PCR-mediated site-directed mutagenesis.
abstract: Unlike traditional site-directed mutagenesis, this protocol requires only a single PCR step using full plasmid amplification to generate point mutants. The method can introduce small mutations into promoter sites and is even better suited for introducing single or double mutations into proteins. It is elegant in its simplicity and can be applied quite easily in any laboratory using standard protein expression vectors and commercially available reagents.
authors.forename: Michael F
authors.initials: MF
authors.lastname: Carey
authors.forename: Craig L
authors.initials: CL
authors.lastname: Peterson
authors.forename: Stephen T
authors.initials: ST
authors.lastname: Smale
mesh_terms: D016297:Mutagenesis, Site-Directed / Q000379:methods*; D016366:Open Reading Frames; D010957:Plasmids; D017354:Point Mutation; D016133:Polymerase Chain Reaction / Q000379:methods*; D011401:Promoter Regions, Genetic