title: Transdentinal cytotoxicity of experimental adhesive systems of different hydrophilicity applied to ethanol-saturated dentin.
abstract: The aim of this study was to evaluate the transdentinal cytotoxicity of experimental adhesive systems (EASs) with different hydrophilicity and dentin saturation solutions on odontoblast-like cells. One hundred 0.4-mm-thick dentin discs were mounted in in vitro pulp chambers and assigned to 10 groups. MDPC-23 cells were seeded onto the pulpal side of the discs, incubated for 48h. The EASs with increasing hydrophilicity (R1, R2, R3 and R4) were applied to the occlusal side after etching and saturation of etched dentin with water or ethanol. R0 (no adhesive) served as controls. R1 is a non-solvated hydrophobic blend, R2 is similar to a simplified etch-and-rinse adhesive system and R3 and R4 are similar to self-etching adhesives. After 24h, cell metabolism was evaluated by MTT assay (n=8 discs) and cell morphology was examined by SEM (n=2 discs). Type of cell death was identified by flow cytometry and the degree of monomer conversion (%DC) was determined by infrared spectroscopy (FTIR) after 10s or 20s of photoactivation. Data were analyzed by the Kruskal-Wallis and Mann-Whitney tests (α=0.05). Dentin saturation with ethanol resulted in higher necrotic cell death ratios for R2, R3 and R4 compared with water saturation, although R2 and R3 induced higher SDH production. Photoactivation for 20s significantly improved the %DC of all EASs compared with 10s. A significant positive correlation was observed between the degree of hydrophilicity and %DC. In conclusion, except for R1, dentin saturation with ethanol increased the cytotoxicity of EASs, as expressed by the induction of necrotic cell death.
authors.affiliation: UNESP - Univ. Estadual Paulista, Araraquara School of Dentistry, Department of Pediatric Dentistry and Orthodontics, Araraquara, SP, Brazil.
authors.forename: Luciana
authors.initials: L
authors.lastname: Bianchi
authors.forename: Ana Paula Dias
authors.initials: AP
authors.lastname: Ribeiro
authors.forename: Marcela Rocha
authors.initials: MR
authors.lastname: de Oliveira Carrilho
authors.forename: David H
authors.initials: DH
authors.lastname: Pashley
authors.forename: Carlos Alberto
authors.initials: CA
authors.lastname: de Souza Costa
authors.forename: Josimeri
authors.initials: J
authors.lastname: Hebling
mesh_terms: D000134:Acid Etching, Dental / Q000379:methods; D000818:Animals; D017438:Bisphenol A-Glycidyl Methacrylate / Q000737:chemistry / Q000633:toxicity; D018929:Cell Culture Techniques; D016923:Cell Death / Q000187:drug effects; D002460:Cell Line; D048430:Cell Shape / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D001840:Dental Bonding / Q000379:methods; D003804:Dentin / Q000187:drug effects*; D003806:Dentin Permeability / Q000187:drug effects; D017220:Dentin-Bonding Agents / Q000737:chemistry / Q000633:toxicity*; D000431:Ethanol / Q000494:pharmacology*; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D055115:Light-Curing of Dental Adhesives; D008689:Methacrylates / Q000737:chemistry / Q000633:toxicity; D051379:Mice; D009336:Necrosis; D009804:Odontoblasts / Q000187:drug effects*; D010755:Organophosphates / Q000737:chemistry / Q000633:toxicity; D011092:Polyethylene Glycols / Q000737:chemistry / Q000633:toxicity; D058105:Polymerization; D011109:Polymethacrylic Acids / Q000737:chemistry / Q000633:toxicity; D012997:Solvents / Q000494:pharmacology*; D014867:Water / Q000737:chemistry; D062366:para-Aminobenzoates / Q000737:chemistry / Q000633:toxicity
chemical_list: C000595499:2-methacryloyloxyethyl dihydrogen phosphate; D017220:Dentin-Bonding Agents; D008689:Methacrylates; D010755:Organophosphates; D011109:Polymethacrylic Acids; D012997:Solvents; C523330:ethyl 4-N,N-dimethylaminobenzoate; D062366:para-Aminobenzoates; D014867:Water; C020946:triethylene glycol dimethacrylate; D000431:Ethanol; D011092:Polyethylene Glycols; D017438:Bisphenol A-Glycidyl Methacrylate; C005044:hydroxyethyl methacrylate
keywords: Adhesive systems; Cytotoxicity; Dentin; Ethanol; Odontoblast-like cells
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