title: Identification and characterization of a novel IκB-ε-like gene from lamprey (Lampetra japonica) with a role in immune response.
abstract: Nuclear factor of kappa B (NF-κB) is a stimuli-activated transcription factor, regulates the expression of a diverse array of genes. Inhibitor of kappa B-epsilon (IκB-ε) is an inhibitor of NF-κB, which retains NF-κB in an inactive state in the cytoplasm. Lampreys (Lampetra japonica) belong to the lowest class of vertebrates with little information about its IκBs. We have identified a cDNA sequence IκB-ε-like in the lamprey and the deduced amino acid sequence of IκB-ε-like. It contains a conserved DSGxxS motif and six consecutive ankyrin repeats, which are necessary for signal-induced degradation of the molecule. Phylogenetic analysis indicated it had high sequence homology with IκB-εs from other vertebrates. FACS analysis showed that IκB-ε-like located in cytoplasm of leukocytes. The degradation of IκB-ε-like could be observed in leukocytes of L. japonica stimulated with lipopolysaccharide. These results indicate that IκB-ε proteins are conserved across vertebrates and the NF-κB-like signaling pathway may exist in the oldest agnatha.
authors.affiliation: College of Life Science, Liaoning Normal University, Dalian 116029, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Su
authors.forename: Xin
authors.initials: X
authors.lastname: Liu
authors.forename: Yinglun
authors.initials: Y
authors.lastname: Han
authors.forename: Zhen
authors.initials: Z
authors.lastname: Zheng
authors.forename: Ge
authors.initials: G
authors.lastname: Liu
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.forename: Qingwei
authors.initials: Q
authors.lastname: Li
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015153:Blotting, Western / Q000662:veterinary; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics / Q000378:metabolism; D029941:Fish Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D020672:I-kappa B Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D007798:Lampreys / Q000235:genetics* / Q000276:immunology* / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000662:veterinary; D017434:Protein Structure, Tertiary; D012313:RNA / Q000235:genetics / Q000378:metabolism; D011817:Rabbits; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment / Q000662:veterinary; D015398:Signal Transduction
chemical_list: D018076:DNA, Complementary; D029941:Fish Proteins; D020672:I-kappa B Proteins; D008070:Lipopolysaccharides; D011994:Recombinant Proteins; D012313:RNA
keywords: IκB-ɛ; Lamprey; NF-κB; Signaling pathway