title: Synthesis of BSA/Fe3O4 magnetic composite microspheres for adsorption of antibiotics.
abstract: BSA/Fe3O4 magnetic composite microspheres with high saturation magnetization and paramagnetic property were prepared via inverse emulsion technology at room temperature, bovine serum albumin (BSA, 60 KD), magnetic nanoparticles (Fe3O4) and glutaraldehyde as macromonomer, inorganic particles and cross-linking agent, respectively. Fourier transform infrared (FTIR), scanning electron microscope (SEM), metalloscope, and particle size analyzer were used to characterize morphology and structure of composite microspheres. Vibrating sample magnetometer (VSM) and thermogravimetric analysis (TGA) were used to test magnetic properties of the synthesized samples, adsorption capacity of microspheres was determined by ultraviolet spectrophotometer (UV). The results showed that BSA/Fe3O4 microspheres were 43 μm with relatively narrow particle size distribution, perfect sphere-shaped morphologies, superparamagnetism with a saturation magnetization of 11 emu/g, and high magnetic content with a value of 57.29%. The main factors influencing properties of microspheres including raw material ratio, the amount of emulsifier and cross-linking agent, agitation speed were investigated and optimized. Furthermore, these microspheres accompanying with high separable and reusable efficient may have great potential application in the field of separation, in particular, removal of antibiotics. Adsorption capacities of the microspheres of four different kinds of antibiotics (erythromycin, streptomycin, tetracycline and chloramphenicol) ranging from 69.35 mg/g to 147.83 mg/g were obtained, and Langmuir isotherm model coincided with equilibrium data than that of the Freundlich model.
authors.affiliation: Department of Applied Chemistry, College of Science, Northwestern Polytechnical University, Xi'an 710072, China.
authors.forename: Baoliang
authors.initials: B
authors.lastname: Zhang
authors.forename: Hepeng
authors.initials: H
authors.lastname: Zhang
authors.forename: Xiangjie
authors.initials: X
authors.lastname: Li
authors.forename: Xingfeng
authors.initials: X
authors.lastname: Lei
authors.forename: Chunmei
authors.initials: C
authors.lastname: Li
authors.forename: Dezhong
authors.initials: D
authors.lastname: Yin
authors.forename: Xinlong
authors.initials: X
authors.lastname: Fan
authors.forename: Qiuyu
authors.initials: Q
authors.lastname: Zhang
mesh_terms: D000327:Adsorption; D000818:Animals; D000900:Anti-Bacterial Agents / Q000302:isolation & purification*; D002417:Cattle; D003432:Cross-Linking Reagents / Q000737:chemistry; D004655:Emulsions; D052203:Ferrosoferric Oxide / Q000138:chemical synthesis*; D060328:Magnetic Phenomena; D008863:Microspheres; D053758:Nanoparticles / Q000648:ultrastructure; D010316:Particle Size; D012710:Serum Albumin, Bovine / Q000378:metabolism*; D013056:Spectrophotometry, Ultraviolet; D017550:Spectroscopy, Fourier Transform Infrared; D013696:Temperature; D013818:Thermogravimetry
chemical_list: D000900:Anti-Bacterial Agents; D003432:Cross-Linking Reagents; D004655:Emulsions; D012710:Serum Albumin, Bovine; D052203:Ferrosoferric Oxide
keywords: Adsorption; Antibiotic; BSA; Magnetic composite microspheres; Protein; Waste-water treatment