title: Cell morphology variations of Klebsiella pneumoniae induced by acetate stress using biomimetic vesicle assay.
abstract: Supplementation with acetate under low levels was used as a novel approach to control the morphological development of Klebsiella pneumoniae aimed to improve 1,3-propanediol (1,3-PD) production. A full range of morphological types formed from rod shape to oval shape even round shape in response to different concentrations of acetate. The cell growth and 1,3-PD productions in the shake flasks with 0.5 g/L acetate addition were improved by 9.4 and 28.37%, respectively, as compared to the control, while the cell became shorter and began to lose its original shape. The cell membrane penetration by acetate was investigated by the biomimetic vesicles, while higher concentration of acetate led to more moderate colorimetric transitions. Moreover, the percentage composition of unsaturated fatty acid (UFA) was increased as well as the increased concentrations of acetate, whereas higher UFA percentage, higher fluidity of bacterial cell membrane.
authors.affiliation: State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, No. 5 Xinmofan Road, Nanjing, Jiangsu, 210009, People's Republic of China.
authors.forename: Shengguo
authors.initials: S
authors.lastname: Lu
authors.forename: Yuwang
authors.initials: Y
authors.lastname: Han
authors.forename: Xujia
authors.initials: X
authors.lastname: Duan
authors.forename: Fang
authors.initials: F
authors.lastname: Luo
authors.forename: Lingyan
authors.initials: L
authors.lastname: Zhu
authors.forename: Shuang
authors.initials: S
authors.lastname: Li
authors.forename: He
authors.initials: H
authors.lastname: Huang
mesh_terms: D000085:Acetates / Q000008:administration & dosage*; D032701:Biomimetics; D002462:Cell Membrane / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism; D005231:Fatty Acids, Unsaturated / Q000494:pharmacology; D007711:Klebsiella pneumoniae / Q000166:cytology* / Q000187:drug effects / Q000254:growth & development; D008560:Membrane Fluidity / Q000187:drug effects; D011409:Propylene Glycols / Q000378:metabolism*; D013312:Stress, Physiological
chemical_list: C493048:10, 12-pentacosadiynoic acid; D000085:Acetates; D005231:Fatty Acids, Unsaturated; D011409:Propylene Glycols; C041787:1,3-propanediol