title: Self-calibrated concentration measurements of polydisperse nanoparticles.
abstract: Quantitative characterization of nanoparticles, e.g. accurate estimation of concentration distributions, is critical to many pharmaceutical and biological applications. We present a method that enables for the first time highly accurate size and absolute concentration measurements of polydisperse nanoparticles in solution, based on fluorescence single particle tracking, that are self-calibrated in the sense that the detection region volume is estimated based on the tracking data. The method is evaluated using simulations and experimental data of polystyrene nanospheres in water/sucrose solution. In addition, the method is used to quantify aggregation and clearance of different types of liposomes after intravenous injection in rats, where additional and more accurate information can be obtained that was previously unavailable, which can help elucidate their usefulness as drug carriers.
authors.affiliation: Department of Mathematical Statistics, Chalmers University of Technology and Gothenburg University, Gothenburg, Sweden.
authors.forename: M
authors.initials: M
authors.lastname: Röding
authors.forename: H
authors.initials: H
authors.lastname: Deschout
authors.forename: K
authors.initials: K
authors.lastname: Braeckmans
authors.forename: A
authors.initials: A
authors.lastname: Särkkä
authors.forename: M
authors.initials: M
authors.lastname: Rudemo
mesh_terms: D061605:Administration, Intravenous; D000818:Animals; D008081:Liposomes / Q000008:administration & dosage* / Q000032:analysis*; D053758:Nanoparticles / Q000032:analysis*; D051381:Rats
chemical_list: D008081:Liposomes
keywords: Concentration; fluorescence single particle tracking; liposomal drug carriers; nanoparticles; polydispersity; size distribution