title: Dexamethasone reduces IL-17 and Tim-3 expression in BALF of asthmatic mice.
abstract: This study investigated the expression of interleukin-17 (IL-17) and T cell immunoglobulin mucin and domain-containing molecule-3 (Tim-3) in bronchoalveolar lavage fluid (BALF) of asthmatic mice and the effect of dexamethasone (DEX) on these factors. Thirty-six mice were randomly divided into three groups: normal group, asthmatic group and DEX group. The mouse model of asthma was established by sensitization with ovalbumin in both the asthmatic and DEX groups. The levels of IL-6, IL-10, IL-17 and TGF-β were measured in BALF by enzyme-linked immunesorbent assay (ELISA). The mRNA expression level of Tim-3 was detected by reverse transcription polymerase chain reaction (RT-PCR). The ratio of Tim-3+CD4+ cells to total CD4+ cells in BALF was determined by flow cytometry. Differential inflammatory cells in BALF were detected. The correlations among IL-17, IL-6, IL-10, Tim-3 and inflammatory cells were analyzed. The results showed that the levels of IL-17, IL-6 and Tim-3 were substantially increased and the IL-10 level decreased in BALF in the asthmatic mice, which was significantly reversed by DEX treatment. IL-17 expression was positively correlated with IL-6 and Tim-3 expression and the number of inflammatory cells but negatively with IL-10 expression. These results indicate that the increased expression of IL-17 and Tim-3 in BALF may be implicated in the occurrence and development of asthmatic inflammation; the mechanism by which DEX suppresses asthmatic airway inflammation involves down-regulation of IL-17 and Tim-3 levels.
authors.affiliation: Department of Pulmonary Medicine, Wuhan Women and Children Health Care Center, Wuhan, 430030, China. lusi74@163.com.
authors.forename: Xiao-Xia
authors.initials: XX
authors.lastname: Lu
authors.affiliation: Department of Pediatrics, Division of Pulmonary Medicine, Nationwide Children's Hospital and The Ohio State University School of Medicine and Public Health, Columbus, 43085, USA.
authors.forename: Karen S
authors.initials: KS
authors.lastname: McCoy
authors.affiliation: Department of Ophthalmology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China.
authors.forename: Wei-Kun
authors.initials: WK
authors.lastname: Hu
authors.affiliation: Department of Pulmonary Medicine, Wuhan Women and Children Health Care Center, Wuhan, 430030, China.
authors.forename: Jia-Li
authors.initials: JL
authors.lastname: Xu
authors.affiliation: Department of Pulmonary Medicine, Wuhan Women and Children Health Care Center, Wuhan, 430030, China.
authors.forename: Hai-Qin
authors.initials: HQ
authors.lastname: Wang
authors.affiliation: Department of Pulmonary Medicine, Wuhan Women and Children Health Care Center, Wuhan, 430030, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Chen
authors.affiliation: Department of Pulmonary Medicine, Wuhan Women and Children Health Care Center, Wuhan, 430030, China.
authors.forename: He-Bin
authors.initials: HB
authors.lastname: Chen
mesh_terms: D000818:Animals; D001249:Asthma / Q000188:drug therapy / Q000235:genetics / Q000378:metabolism*; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry; D003907:Dexamethasone / Q000494:pharmacology*; D005260:Female; D015870:Gene Expression / Q000187:drug effects* / Q000235:genetics; D000072597:Hepatitis A Virus Cellular Receptor 2; D020381:Interleukin-17 / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D011991:Receptors, Virus / Q000235:genetics / Q000378:metabolism*
chemical_list: C442241:Havcr2 protein, mouse; D000072597:Hepatitis A Virus Cellular Receptor 2; D020381:Interleukin-17; D011991:Receptors, Virus; D003907:Dexamethasone
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