title: Oligomers matrix-assisted dispersion of high content of carbon nanotubes into monolithic column for online separation and enrichment of proteins from complex biological samples.
abstract: In this work, a new oligomer matrix-assisted dispersion (OMAD) method for the preparation of homogeneous dispersion of multi-walled carbon nanotubes (MWNTs) incorporated monolithic column was developed. Oligomers matrix as a scaffold could allow MWNTs to entangle with it instead of self-aggregation, so the MWNTs remain in the polymer network followed by in situ self-solidification. The OMAD method not only greatly enlarged the BET surface area of MWNTs incorporated monolithic column from 13.8 m(2) g(-1) to 85.5 m(2) g(-1) without a significant effect on the surface chemistry of the MWNTs, but also improved the dispersion of MWNTs making its content up to 5 wt% (with respect to monomers). The synthesized materials combine the favorable attributes of both high permeability and large surface area, making them excellent candidates for on-line separation and enrichment of proteins. The oligomer matrix-assisted dispersion MWNTs incorporated monolithic columns (OMAD-MMC) exhibited higher enrichment factors and the adsorption capacity is about 5-fold for basic proteins compared with MWNTs incorporated monolithic columns (MMC) prepared by the conventional in situ polymerization. The practical application of OMAD-MMC was proven by selective extraction of hemoglobin in human whole blood samples with SDS-PAGE. On the basis of the results, OMAD as a simple and effective method for dispersion high content MWNTs into monolithic columns shows great promise.
authors.affiliation: School of Chemistry and Chemical Engineering, Sun Yat-sen University, Guangzhou, 510275, China. cesgkl@mail.sysu.edu.cn duzh@mail.sysu.edu.cn.
authors.forename: Chanyuan
authors.initials: C
authors.lastname: Zhou
authors.forename: Zhuo
authors.initials: Z
authors.lastname: Du
authors.forename: Gongke
authors.initials: G
authors.lastname: Li
authors.forename: Yukui
authors.initials: Y
authors.lastname: Zhang
authors.forename: Zongwei
authors.initials: Z
authors.lastname: Cai
mesh_terms: D045304:Cytochromes c / Q000032:analysis* / Q000378:metabolism; D016326:Extracellular Matrix Proteins / Q000032:analysis / Q000378:metabolism; D006454:Hemoglobins / Q000032:analysis* / Q000378:metabolism; D006801:Humans; D037742:Nanotubes, Carbon / Q000737:chemistry*; D058105:Polymerization; D011485:Protein Binding / Q000502:physiology
chemical_list: D016326:Extracellular Matrix Proteins; D006454:Hemoglobins; D037742:Nanotubes, Carbon; D045304:Cytochromes c