title: CHO microRNA engineering is growing up: recent successes and future challenges.
abstract: microRNAs with their ability to regulate complex pathways that control cellular behavior and phenotype have been proposed as potential targets for cell engineering in the context of optimization of biopharmaceutical production cell lines, specifically of Chinese Hamster Ovary cells. However, until recently, research was limited by a lack of genomic sequence information on this industrially important cell line. With the publication of the genomic sequence and other relevant data sets for CHO cells since 2011, the doors have been opened for an improved understanding of CHO cell physiology and for the development of the necessary tools for novel engineering strategies. In the present review we discuss both knowledge on the regulatory mechanisms of microRNAs obtained from other biological models and proof of concepts already performed on CHO cells, thus providing an outlook of potential applications of microRNA engineering in production cell lines.
authors.affiliation: Department of Biotechnology, University of Natural Resources and Life Sciences, Vienna, Austria.
authors.forename: Vaibhav
authors.initials: V
authors.lastname: Jadhav
authors.forename: Matthias
authors.initials: M
authors.lastname: Hackl
authors.forename: Aliaksandr
authors.initials: A
authors.lastname: Druz
authors.forename: Smriti
authors.initials: S
authors.lastname: Shridhar
authors.forename: Cheng-Yu
authors.initials: CY
authors.lastname: Chung
authors.forename: Kelley M
authors.initials: KM
authors.lastname: Heffner
authors.forename: David P
authors.initials: DP
authors.lastname: Kreil
authors.forename: Mike
authors.initials: M
authors.lastname: Betenbaugh
authors.forename: Joseph
authors.initials: J
authors.lastname: Shiloach
authors.forename: Niall
authors.initials: N
authors.lastname: Barron
authors.forename: Johannes
authors.initials: J
authors.lastname: Grillari
authors.forename: Nicole
authors.initials: N
authors.lastname: Borth
mesh_terms: D000818:Animals; D001709:Biotechnology; D016466:CHO Cells; D060846:Cell Engineering; D006224:Cricetinae; D003412:Cricetulus; D035683:MicroRNAs; D011994:Recombinant Proteins
chemical_list: D035683:MicroRNAs; D011994:Recombinant Proteins
keywords: Bioprocess relevant properties; Chinese Hamster Ovary cells; MicroRNA engineering
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