title: Effect of zeta potentials on bovine serum albumin adsorption to hydroxyapatite surfaces.
abstract: The aim of the present study was to examine the adsorption of bovine serum albumin (BSA) to hydroxyapatite surfaces by means of zeta potential. The electrophoretic mobility of both hydroxyapatite and BSA were negative, with BSA itself less negative than hydroxyapatite. The zeta potential of the surface of BSA-adsorbed hydroxyapatite was significantly more negative than that of hydroxyapatite alone (p<0.0001). The BSA histogram indicated two negative peaks, and the zeta potential of BSA-adsorbed hydroxyapatite also showed two similar negative peaks. These results suggest that BSA adsorption to hydroxyapatite surfaces is related to electrostatic interaction.
authors.affiliation: Division of Prosthodontics, Department of Clinical Oral Health Science, Tokyo Dental College, 2-9-18 Misaki-cho, Chiyoda-ku, Tokyo 101-0061, Japan. miyake@tdc.ac.jp
authors.forename: Nahoko
authors.initials: N
authors.lastname: Miyake
authors.forename: Toru
authors.initials: T
authors.lastname: Sato
authors.forename: Yoshinobu
authors.initials: Y
authors.lastname: Maki
mesh_terms: D000327:Adsorption; D000818:Animals; D002417:Cattle; D017886:Durapatite / Q000737:chemistry*; D024202:Electrophoretic Mobility Shift Assay; D008863:Microspheres; D011137:Polystyrenes / Q000737:chemistry; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D012965:Sodium Chloride / Q000737:chemistry; D013053:Spectrophotometry / Q000379:methods; D055672:Static Electricity; D013499:Surface Properties
chemical_list: D011137:Polystyrenes; D012710:Serum Albumin, Bovine; D012965:Sodium Chloride; C024075:styrofoam; D017886:Durapatite