title: The receptor-like protein ReMAX of Arabidopsis detects the microbe-associated molecular pattern eMax from Xanthomonas.
abstract: As part of their immune system, plants have pattern recognition receptors (PRRs) that can detect a broad range of microbe-associated molecular patterns (MAMPs). Here, we identified a PRR of Arabidopsis thaliana with specificity for the bacterial MAMP eMax from xanthomonads. Response to eMax seems to be restricted to the Brassicaceae family and also varied among different accessions of Arabidopsis. In crosses between sensitive accessions and the insensitive accession Shakhdara, eMax perception mapped to receptor-like protein1 (RLP1). Functional complementation of rlp1 mutants required gene constructs that code for a longer version of RLP1 that we termed ReMAX (for receptor of eMax). ReMAX/RLP1 is a typical RLP with structural similarity to the tomato (Solanum lycopersicum) RLP Eix2, which detects fungal xylanase as a MAMP. Attempts to demonstrate receptor function by interfamily transfer of ReMAX to Nicotiana benthamiana were successful after using hybrid receptors with the C-terminal part of ReMAX replaced by that of Eix2. These results show that ReMAX determines specificity for eMax. They also demonstrate hybrid receptor technology as a promising tool to overcome problems that impede interfamily transfer of PRRs to enhance pathogen detection in crop plants.
authors.affiliation: Zentrum für Molekularbiologie der Pflanzen, University Tübingen, 72076 Tuebingen, Germany.
authors.forename: Anna Kristina
authors.initials: AK
authors.lastname: Jehle
authors.forename: Martin
authors.initials: M
authors.lastname: Lipschis
authors.forename: Markus
authors.initials: M
authors.lastname: Albert
authors.forename: Vahid
authors.initials: V
authors.lastname: Fallahzadeh-Mamaghani
authors.forename: Ursula
authors.initials: U
authors.lastname: Fürst
authors.forename: Katharina
authors.initials: K
authors.lastname: Mueller
authors.forename: Georg
authors.initials: G
authors.lastname: Felix
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism / Q000382:microbiology; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism; D054884:Host-Pathogen Interactions / Q000235:genetics; D018551:Solanum lycopersicum / Q000235:genetics / Q000378:metabolism / Q000382:microbiology; D008957:Models, Genetic; D008969:Molecular Sequence Data; D009154:Mutation; D018515:Plant Leaves / Q000235:genetics / Q000378:metabolism / Q000382:microbiology; D011523:Protoplasts / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D014026:Nicotiana / Q000235:genetics / Q000378:metabolism / Q000382:microbiology; D014974:Xanthomonas / Q000235:genetics* / Q000378:metabolism / Q000502:physiology
chemical_list: D029681:Arabidopsis Proteins; D001426:Bacterial Proteins; D002352:Carrier Proteins; C586779:RLP1 protein, Arabidopsis
references.citation: Plant Physiol. 2008 Jun;147(2):503-17
references.pmid: 18434605
references.citation: Proc Natl Acad Sci U S A. 2012 Mar 27;109(13):5110-5
references.pmid: 22416119
references.citation: Science. 1994 Nov 4;266(5186):789-93
references.pmid: 7973631
references.citation: Proc Natl Acad Sci U S A. 2011 Dec 6;108(49):19824-9
references.pmid: 22106285
references.citation: Nat Protoc. 2007;2(7):1565-72
references.pmid: 17585298
references.citation: Plant Cell. 2006 Mar;18(3):764-79
references.pmid: 16461584
references.citation: Mol Biol Evol. 2012 Jun;29(6):1655-67
references.pmid: 22319159
references.citation: Plant Physiol. 1999 Oct;121(2):345-51
references.pmid: 10517825
references.citation: Cell. 2006 May 19;125(4):749-60
references.pmid: 16713565
references.citation: Science. 2007 Jul 20;317(5836):338-42
references.pmid: 17641193
references.citation: Proc Natl Acad Sci U S A. 2007 Dec 4;104(49):19613-8
references.pmid: 18042724
references.citation: Plant Physiol. 2005 Jun;138(2):611-23
references.pmid: 15955925
references.citation: Plant Physiol. 2004 May;135(1):432-43
references.pmid: 15122038
references.citation: Mol Gen Genet. 1989 Jul;218(1):127-36
references.pmid: 2550761
references.citation: Plant Cell. 1999 Oct;11(10):1925-34
references.pmid: 10521522
references.citation: Cell. 1997 Sep 5;90(5):929-38
references.pmid: 9298904
references.citation: Annu Rev Phytopathol. 2000 Sep;38:241-261
references.pmid: 11701843
references.citation: Mol Cell. 2000 Jun;5(6):1003-11
references.pmid: 10911994
references.citation: Science. 2002 May 31;296(5573):1697-700
references.pmid: 12040198
references.citation: Plant Cell. 2003 Nov;15(11):2551-65
references.pmid: 14555693
references.citation: Arabidopsis Book. 2002;1:e0039
references.pmid: 22303207
references.citation: Plant Physiol. 2000 Aug;123(4):1247-56
references.pmid: 10938344
references.citation: Annu Rev Plant Biol. 2009;60:379-406
references.pmid: 19400727
references.citation: Plant Physiol. 2010 Jan;152(1):166-76
references.pmid: 19933383
references.citation: Nat Biotechnol. 2010 Apr;28(4):365-9
references.pmid: 20231819
references.citation: Plant Cell. 2005 Mar;17(3):1000-15
references.pmid: 15722474
references.citation: Plant Cell. 2004 Jun;16(6):1604-15
references.pmid: 15155877
references.citation: Plant J. 2003 Mar;33(5):949-56
references.pmid: 12609035
references.citation: Plant Mol Biol. 1994 Sep;25(6):989-94
references.pmid: 7919218
references.citation: Plant Physiol. 2011 Aug;156(4):2255-65
references.pmid: 21617027
references.citation: J Biotechnol. 2006 Oct 20;126(1):78-86
references.pmid: 16603270
references.citation: Genome Res. 2006 Jun;16(6):787-95
references.pmid: 16702412
references.citation: Plant Physiol. 1999 Oct;121(2):391-7
references.pmid: 10517830
references.citation: J Biol Chem. 2010 Jun 18;285(25):19035-42
references.pmid: 20410299
references.citation: J Biol Chem. 2007 Nov 2;282(44):32338-48
references.pmid: 17761682
references.citation: Plant Physiol. 2010 Jan;152(1):320-31
references.pmid: 19897604
references.citation: Theor Appl Genet. 2002 May;104(6-7):1173-1184
references.pmid: 12582628
references.citation: Plant J. 1999 May;18(3):265-76
references.pmid: 10377992
references.citation: Cell. 2006 Feb 24;124(4):783-801
references.pmid: 16497588
references.citation: Plant Cell. 2004 Dec;16(12):3496-507
references.pmid: 15548740
references.citation: Plant J. 1999 May;18(3):277-84
references.pmid: 10377993
references.citation: Plant Cell. 2012 May;24(5):2213-24
references.pmid: 22634763
references.citation: Nature. 2006 Nov 16;444(7117):323-9
references.pmid: 17108957
references.citation: EMBO J. 2009 Nov 4;28(21):3428-38
references.pmid: 19763086
references.citation: Nature. 2002 May 23;417(6887):459-63
references.pmid: 12024217
references.citation: Nature. 2004 Apr 15;428(6984):764-7
references.pmid: 15085136
references.citation: Nature. 2002 Feb 28;415(6875):977-83
references.pmid: 11875555
references.citation: Mol Plant Microbe Interact. 2009 Oct;22(10):1203-13
references.pmid: 19737094