title: Transgelin: an androgen-dependent protein identified in the seminal vesicles of three Saharan rodents.
abstract: During the breeding season, a major androgen-dependent protein with an apparent molecular weight of 21 kDa was isolated and purified from the seminal vesicles of three Saharan rodents (MLVSP21 from Meriones libycus, MSVSP21 from Meriones shawi, and MCVSP21 from Meriones crassus). The 21-kDa protein was isolated and purified from soluble seminal vesicle proteins of homogenate by one-dimensional polyacrylamide gel electrophoresis (SDS-PAGE). Using polyclonal antibodies directed against POSVP21 (Psammomys obesus seminal vesicles protein of 21 kDa), a major androgen-dependent secretory protein from sand rat seminal vesicles, identified previously as transgelin, we showed an immunological homology with POSVP21 by immunoblotting. These three major androgen-dependent proteins with a same apparent molecular weight of 21 kDa designated as MLVSP21 (Meriones libycus seminal vesicles protein of 21 kDa), MSVSP21 (Meriones shawi seminal vesicles protein of 21 kDa), and MCVSP21 (Meriones crassus seminal vesicles protein of 21 kDa) were localized by immunohistochemistry and identified by applying a proteomic approach. Our results indicated that the isolated proteins MLSVP21, MSSVP21, and MCSVP21 seem to correspond to the same protein: the transgelin. So that transgelin can be used as a specific marker of these rodent physiological reproduction mechanisms.
authors.affiliation: Laboratory of Arid Areas, Biological Sciences Institute, USTHB, Algiers, Algeria. Electronic address: kacinaima@yahoo.fr.
authors.forename: Naïma
authors.initials: N
authors.lastname: Kaci-Ouchfoun
authors.forename: Djamila
authors.initials: D
authors.lastname: Izemrane
authors.forename: Abdelkrim
authors.initials: A
authors.lastname: Boudrissa
authors.forename: Thérèse
authors.initials: T
authors.lastname: Gernigon
authors.forename: Farida
authors.initials: F
authors.lastname: Khammar
authors.forename: Jean Marie
authors.initials: JM
authors.lastname: Exbrayat
mesh_terms: D000818:Animals; D005849:Gerbillinae / Q000378:metabolism*; D008297:Male; D008840:Microfilament Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D009124:Muscle Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D012669:Seminal Vesicles / Q000378:metabolism*; D013045:Species Specificity
chemical_list: D008840:Microfilament Proteins; D009124:Muscle Proteins; C051615:transgelin
keywords: Androgen-dependent proteins; Desert; Reproduction; Rodent; Seminal vesicles; Transgelin