title: Stabilizing gold nanoparticle bioconjugates in physiological conditions by PEGylation.
abstract: Stability of gold nanoparticles (AuNPs) is often compromised in physiological conditions. The loss of colloidal stability might lead to undesired biological responses for drug delivery nanosystems. Here a methodology to confer additional stability to the AuNPs by the addition of PEG is presented. Also, protocols to prepare and characterize the composition and conformation of mixed layers with PEG and alkanethiols are described here. Finally, methods to assay the stability of the link between the NP conjugates and a model drug are shown.
authors.affiliation: Catalan Institute of Nanotechnology, Universitat Autònoma de Barcelona, Barcelona, Spain.
authors.forename: Joan
authors.initials: J
authors.lastname: Comenge
authors.forename: Víctor F
authors.initials: VF
authors.lastname: Puntes
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry; D002945:Cisplatin / Q000737:chemistry; D016503:Drug Delivery Systems; D006046:Gold / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D008968:Molecular Conformation; D053758:Nanoparticles / Q000737:chemistry*; D036103:Nanotechnology / Q000379:methods*; D011092:Polyethylene Glycols / Q000737:chemistry
chemical_list: D001672:Biocompatible Materials; D011092:Polyethylene Glycols; D006046:Gold; D002945:Cisplatin