title: Effects of non-surgical periodontal treatment on the L-arginine-nitric oxide pathway and oxidative status in platelets.
abstract: Several studies have suggested an increase of cardiovascular disease (CVD) risk on periodontitis patients. An enhancement has been demonstrated on both platelet activation and oxidative stress on periodontitis patients, which may contribute for this association. Therefore, the aim of this study was to evaluate the effects of non-surgical periodontal treatment on the l-arginine-nitric oxide (NO)-cyclic guanosine monophosphate (cGMP) pathway and oxidative status in platelets. A total of eight periodontitis patients and eight controls were included in this study. Clinical, laboratory and experimental evaluations were performed on baseline and 90 days after periodontal treatment (except for western blot analysis). The clinical periodontal evaluation included measurements of probing pocket depth (PPD), clinical attachment loss (CAL), % of sites with plaque and % of sites with bleeding on probing. We evaluated: l-[(3)H]arginine influx; nitric oxide synthase (NOS) and arginase enzymes activity and expression; expression of guanylate cyclase and phosphodiesterase-5 enzymes; cGMP levels; platelet aggregation; oxidative status through superoxide dismutase (SOD) and catalase activities, and measurement of reactive oxygen species (ROS) levels and C-reactive protein (CRP) levels. The initial results showed an activation of both l-arginine influx and via system y (+ )L associated with reduced intraplatelet cGMP levels in periodontitis patients and increased systemic levels of CRP. After periodontal treatment, there was a significant reduction of the % of sites with PPD 4-5mm, % of sites with CAL 4-5 mm, and an enhancement in cGMP levels and SOD activity. Moreover, CRP levels were reduced after treatment. Therefore, alterations in the intraplatelet l-arginine-NO-cGMP pathway and oxidant-antioxidant balance associated with a systemic inflammatory response may lead to platelet dysfunction, which may contribute to a higher risk of CVD in periodontitis.
authors.affiliation: Department of Periodontology, Faculty of Odontology, Rio de Janeiro State University, Rio de Janeiro, CEP: 20551-030, Brazil.
authors.forename: Mariana Alves de Sá
authors.initials: MA
authors.lastname: Siqueira
authors.forename: Ricardo Guimarães
authors.initials: RG
authors.lastname: Fischer
authors.forename: Natália Rodrigues
authors.initials: NR
authors.lastname: Pereira
authors.forename: Marcela Anjos
authors.initials: MA
authors.lastname: Martins
authors.forename: Monique Bandeira
authors.initials: MB
authors.lastname: Moss
authors.forename: Antônio Cláudio
authors.initials: AC
authors.lastname: Mendes-Ribeiro
authors.forename: Carlos Marcelo da Silva
authors.initials: CM
authors.lastname: Figueredo
authors.forename: Tatiana Marlowe Cunha
authors.initials: TM
authors.lastname: Brunini
mesh_terms: D000328:Adult; D001120:Arginine / Q000378:metabolism*; D001792:Blood Platelets / Q000378:metabolism*; D002097:C-Reactive Protein / Q000378:metabolism; D002318:Cardiovascular Diseases / Q000150:complications / Q000378:metabolism; D006152:Cyclic GMP / Q000378:metabolism; D006801:Humans; D008875:Middle Aged; D009569:Nitric Oxide / Q000378:metabolism*; D019001:Nitric Oxide Synthase / Q000378:metabolism; D018384:Oxidative Stress / Q000502:physiology*; D010518:Periodontitis / Q000150:complications / Q000378:metabolism*; D015539:Platelet Activation / Q000502:physiology; D010974:Platelet Aggregation / Q000502:physiology; D015398:Signal Transduction / Q000502:physiology
chemical_list: D009569:Nitric Oxide; D002097:C-Reactive Protein; D001120:Arginine; D019001:Nitric Oxide Synthase; D006152:Cyclic GMP
keywords: l-arginine-NO-cGMP pathway; oxidative status; periodontitis; platelets