title: [Improved production of microbial lipids in the two-liquid phase fermentation system].
abstract: In the present study, we developed a two-liquid phase fermentation system by adding 1% n-dodecane as oxygen-vector to enhance the microbial lipids productivity of Trichosporon fermentans using cassava starch hydrolysate. Results suggest that the oxygen-vector could alleviate the oxygen shortage in flask fermentation. The cell mass and lipids concentration were 101.2 g/L and 50.28 respectively in 2 L fermenter with the presence of 1% n-dodecane. Additionally, gas chromatography analysis also reveals that the microbial lipids produced by T. fermentans contained a higher percentage of saturated fatty acid in the oxygen-vector case.
authors.affiliation: Key Laboratory of Protection and Utilization of Subtropic Plant Resources of Jiangxi Province, College of Life Sciences, Jiangxi Normal University, Nanchang 330022, Jiangxi, China.
authors.forename: Riming
authors.initials: R
authors.lastname: Yan
authors.forename: Zuozuo
authors.initials: Z
authors.lastname: Ai
authors.forename: Ya
authors.initials: Y
authors.lastname: Wang
authors.forename: Zhibin
authors.initials: Z
authors.lastname: Zhang
authors.forename: Qinggui
authors.initials: Q
authors.lastname: Zeng
authors.forename: Zhu
authors.initials: Z
authors.lastname: Du
mesh_terms: D000473:Alkanes / Q000737:chemistry; D056804:Biofuels; D005285:Fermentation; D007218:Industrial Microbiology / Q000379:methods; D008055:Lipids / Q000096:biosynthesis*; D002365:Manihot / Q000378:metabolism*; D013213:Starch / Q000378:metabolism; D014250:Trichosporon / Q000235:genetics / Q000378:metabolism*
chemical_list: D000473:Alkanes; D056804:Biofuels; D008055:Lipids; C007548:n-dodecane; D013213:Starch