title: Effect of a calcium-phosphate based desensitizer on dentin surface characteristics.
abstract: This study aimed to evaluate the ability of a newly developed calcium-phosphate desensitizer in dentin permeability reduction and its integration with dentin surface before and after immersion in artificial saliva (AS) under two different dentin surface characteristics; with or without the collagen exposure.Humandentin discs treated by EDTA to expose collagen fibrils or EDTA/NaOCl to expose plain dentin surface were subjected to a calcium-phosphate desensitizer (Teethmate Desensitizer; TMD), while non-desensitizer treatment served as control. TMD application showed the occlusion in dentinal tubules and reduction in dentin permeability up to 92%, regardless of dentin surface characteristics. After AS immersion, permeability reduction percent (PR%) significantly increased in EDTA/NaOCl pretreatment (p<0.05). Newly-formed crystallites were observed on desensitizer treated dentin and EDTA/NaOCl pretreatment control group, whereas the crystallites did not exist on EDTA pretreatment control group. Ultrasonication revealed the integration of the calcium-phosphate rich layer of desensitizer on dentin surface after AS immersion.
authors.affiliation: Cariology and Operative Dentistry, Department of Oral Health Sciences, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, 1-5-45, Yushima, Bunkyo-ku, Tokyo 113-8549, Japan.
authors.forename: Ornnicha
authors.initials: O
authors.lastname: Thanatvarakorn
authors.forename: Syozi
authors.initials: S
authors.lastname: Nakashima
authors.forename: Alireza
authors.initials: A
authors.lastname: Sadr
authors.forename: Taweesak
authors.initials: T
authors.lastname: Prasansuttiporn
authors.forename: Suppason
authors.initials: S
authors.lastname: Thitthaweerat
authors.forename: Junji
authors.initials: J
authors.lastname: Tagami
mesh_terms: D002130:Calcium Phosphates / Q000494:pharmacology*; D002614:Chelating Agents / Q000494:pharmacology; D003094:Collagen / Q000187:drug effects / Q000648:ultrastructure; D003460:Crystallization; D003804:Dentin / Q000187:drug effects* / Q000648:ultrastructure; D057133:Dentin Desensitizing Agents / Q000494:pharmacology*; D003806:Dentin Permeability / Q000187:drug effects; D004492:Edetic Acid / Q000494:pharmacology; D006801:Humans; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D016877:Oxidants / Q000494:pharmacology; D012464:Saliva, Artificial / Q000737:chemistry; D016369:Smear Layer; D012973:Sodium Hypochlorite / Q000494:pharmacology; D014465:Ultrasonics
chemical_list: D002130:Calcium Phosphates; D002614:Chelating Agents; D057133:Dentin Desensitizing Agents; D016877:Oxidants; D012464:Saliva, Artificial; C485830:tetracalcium phosphate; D003094:Collagen; C020243:calcium phosphate; D004492:Edetic Acid; D012973:Sodium Hypochlorite; C485829:calcium phosphate, dibasic, anhydrous