title: Random-access optical-resolution photoacoustic microscopy using a digital micromirror device.
abstract: We developed random-access optical-resolution photoacoustic microscopy using a digital micromirror device. This system can rapidly scan arbitrarily shaped regions of interest within a 40 μm×40 μm imaging area with a lateral resolution of 3.6 μm. To identify a region of interest, a global structural image is first acquired, then the selected region is scanned. The random-access ability was demonstrated by imaging two static samples, a carbon fiber cross and a monolayer of red blood cells, with an acquisition rate up to 4 kHz. The system was then used to monitor blood flow in vivo in real time within user-selected capillaries in a mouse ear. By imaging only the capillary of interest, the frame rate was increased by up to 9.2 times.
authors.affiliation: Optical Imaging Laboratory, Department of Biomedical Engineering, Washington University in St. Louis, St. Louis, Missouri 63130, USA.
authors.forename: Jinyang
authors.initials: J
authors.lastname: Liang
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhou
authors.forename: Amy W
authors.initials: AW
authors.lastname: Winkler
authors.forename: Lidai
authors.initials: L
authors.lastname: Wang
authors.forename: Konstantin I
authors.initials: KI
authors.lastname: Maslov
authors.forename: Chiye
authors.initials: C
authors.lastname: Li
authors.forename: Lihong V
authors.initials: LV
authors.lastname: Wang
mesh_terms: D000818:Animals; D004423:Ear / Q000098:blood supply; D051379:Mice; D008853:Microscopy / Q000295:instrumentation*; D055096:Optical Devices; D061088:Photoacoustic Techniques / Q000295:instrumentation*
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