title: Standardization of multilocus sequence typing scheme for Mycobacterium abscessus and Mycobacterium massiliense.
abstract: This study aims to develop a multilocus sequence typing (MLST) scheme for Mycobacterium abscessus complex for the typing of stains within each species. A total of 89 clinical isolates of M. abscessus complex from 71 patients of 2 tertiary care hospitals in South Korea were included. Forty-two isolates were identified as M. abscessus, and 29, as Mycobacterium massiliense through sequencing of 8 housekeeping genes and rpoB. The MLST scheme identified 26 different sequence types(STs) and 13 different clonal complexes (CCs) in M. abscessus and 12 different STs and 6 different CCs in M. massiliense. The MLST data showed high concordance with the XbaI-macrorestriction patterns of pulsed-field gel electrophoresis in the duplicated isolates. Our MLST schemes could identify different strains of M. abscessus and M. massiliense, and the schemes also showed a reliable reproducibility. Therefore, our MLST schemes may be useful in studying the epidemiology of M. abscessus and M. massiliense infections.
authors.affiliation: Division of Pulmonology, Department of Internal Medicine, Yonsei University College of Medicine, Seoul 120-752, Republic of Korea.
authors.forename: Song Yee
authors.initials: SY
authors.lastname: Kim
authors.forename: Young Ae
authors.initials: YA
authors.lastname: Kang
authors.forename: Il Kwon
authors.initials: IK
authors.lastname: Bae
authors.forename: Jae-Joon
authors.initials: JJ
authors.lastname: Yim
authors.forename: Moo Suk
authors.initials: MS
authors.lastname: Park
authors.forename: Young Sam
authors.initials: YS
authors.lastname: Kim
authors.forename: Se Kyu
authors.initials: SK
authors.lastname: Kim
authors.forename: Joon
authors.initials: J
authors.lastname: Chang
authors.forename: Seok Hoon
authors.initials: SH
authors.lastname: Jeong
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D005260:Female; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D058885:Multilocus Sequence Typing / Q000379:methods* / Q000592:standards*; D009161:Mycobacterium / Q000145:classification* / Q000235:genetics; D009164:Mycobacterium Infections / Q000382:microbiology*; D012189:Retrospective Studies
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial
keywords: Multilocus sequence analysis; Multilocus sequence typing; Mycobacterium abscessus; Mycobacterium massiliense; rpoB