title: Chryseobacterium gwangjuense sp. nov., isolated from soil.
abstract: A Gram-stain-negative, strictly aerobic, non-motile, rod-shaped bacterial strain, THG-A18(T), was isolated from soil of Gwangju province in South Korea. Strain THG-A18(T) grew optimally at 25-30 °C, at pH 7.0-8.0 and in the absence of NaCl. Strain THG-A18(T) displayed β-glucosidase activity, which enabled it to convert ginsenoside Rb1 to Rd. According to 16S rRNA gene sequence analysis, strain THG-A18(T) was shown to belong to the genus Chryseobacterium. The closest phylogenetic neighbours were Chryseobacterium ginsenosidimutans THG 15(T) (97.9 % 16S rRNA gene sequence similariity), C. defluvii B2(T) (97.7 %), C. daeguense K105(T) (97.6 %), C. taiwanense BCRC 17412(T) (97.5 %), C. indoltheticum LMG 4025(T) (97.4 %), C. gregarium P 461/12(T) (97.4 %) and C. lathyri RBA2-6(T) (97.3 %), but DNA-DNA relatedness values between these strains and strain THG-A18(T) were below 41.9 %. The G+C content of the genomic DNA was 36.4 mol%. The major respiratory quinone (MK-6) and fatty acids [iso-C15 : 0, iso-C17 : 0 3-OH, summed feature 3 (comprising C16 : 1ω7c and/or C16 : 1ω6c) and summed feature 9 (comprising iso-C17 : 1ω9c and/or 10-methyl C16 : 0)] supported the affiliation of strain THG-A18(T) with the genus Chryseobacterium. The polar lipids of strain THG-A18(T) were phosphatidylethanolamine, four unidentified aminolipids and seven unidentified lipids. A number of physiological and biochemical tests allowed phenotypic differentiation of strain THG-A18(T) from recognized species of the genus Chryseobacterium. The name Chryseobacterium gwangjuense sp. nov. is proposed, with THG-A18(T) ( = KACC 16227(T) = LMG 26579(T)) as the type strain.
authors.affiliation: Department of Oriental Medicinal Material & Processing College of Life Science, KyungHee University, Yongin, Kyunggi 446-701, Republic of Korea.
authors.forename: Yong Jin
authors.initials: YJ
authors.lastname: Park
authors.affiliation: Department of Oriental Medicinal Material & Processing College of Life Science, KyungHee University, Yongin, Kyunggi 446-701, Republic of Korea.
authors.forename: Heung-Min
authors.initials: HM
authors.lastname: Son
authors.affiliation: Department of Oriental Medicinal Material & Processing College of Life Science, KyungHee University, Yongin, Kyunggi 446-701, Republic of Korea.
authors.forename: Eun-Hee
authors.initials: EH
authors.lastname: Lee
authors.affiliation: Department of Oriental Medicinal Material & Processing College of Life Science, KyungHee University, Yongin, Kyunggi 446-701, Republic of Korea.
authors.forename: Ju Han
authors.initials: JH
authors.lastname: Kim
authors.affiliation: Department of Oriental Medicinal Material & Processing College of Life Science, KyungHee University, Yongin, Kyunggi 446-701, Republic of Korea.
authors.forename: Gafurjon T
authors.initials: GT
authors.lastname: Mavlonov
authors.affiliation: Division of Respiratory Viruses, Center for Infectious Diseases, National Institute of Health, South Korea CDC, Osong Health Technology Administration Complex, Cheongwon-gun, Chungbuk, 363-951, Republic of Korea.
authors.forename: Ki Ju
authors.initials: KJ
authors.lastname: Choi
authors.affiliation: Department of Oriental Medicinal Material & Processing College of Life Science, KyungHee University, Yongin, Kyunggi 446-701, Republic of Korea.
authors.forename: Hun-Sub
authors.initials: HS
authors.lastname: Shin
authors.affiliation: Department of Marine Biotechnology, Anyang University, Incheon 417-833, Republic of Korea.
authors.forename: MooChang
authors.initials: M
authors.lastname: Kook
authors.affiliation: Department of Oriental Medicinal Material & Processing College of Life Science, KyungHee University, Yongin, Kyunggi 446-701, Republic of Korea.
authors.forename: Tae-Hoo
authors.initials: TH
authors.lastname: Yi
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D045247:Chryseobacterium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D036145:Ginsenosides / Q000378:metabolism; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010714:Phosphatidylethanolamines / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D036145:Ginsenosides; D010714:Phosphatidylethanolamines; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C483858:phosphatidylethanolamine; C042455:menaquinone 6; C442759:ginsenoside Rb1