title: Rapid detection of carbapenemase-producing Enterobacteriaceae from blood cultures.
abstract: The biochemical-based Carba NP test has been evaluated to detect carbapenemase-producing Enterobacteriaceae (n = 193) directly from spiked blood cultures. It was able to rapidly detect KPC (n = 50), IMP (n = 27), VIM (n = 37), NDM (n = 33) and OXA-48-like producers (n = 46) with sensitivity and specificity of 97.9% and 100%, respectively. This cost-effective technique may be implemented in any microbiology laboratory and offers a reliable test for an early identification of carbapenemase-producing Enterobacteriaceae directly from blood culture that could be useful for the management of infected patients.
authors.affiliation: Service de Bactériologie-Virologie, INSERM U914 "Emerging Resistance to Antibiotics", Hôpital de Bicêtre, Assistance Publique/Hôpitaux de Paris, Faculté de Médecine Paris Sud, K.-Bicêtre, France.
authors.forename: L
authors.initials: L
authors.lastname: Dortet
authors.forename: L
authors.initials: L
authors.lastname: Bréchard
authors.forename: L
authors.initials: L
authors.lastname: Poirel
authors.forename: P
authors.initials: P
authors.lastname: Nordmann
mesh_terms: D016470:Bacteremia / Q000382:microbiology*; D001426:Bacterial Proteins / Q000032:analysis*; D001431:Bacteriological Techniques / Q000191:economics / Q000379:methods*; D001769:Blood / Q000382:microbiology*; D003362:Cost-Benefit Analysis; D004755:Enterobacteriaceae / Q000201:enzymology* / Q000302:isolation & purification*; D004756:Enterobacteriaceae Infections / Q000382:microbiology*; D006801:Humans; D012680:Sensitivity and Specificity; D001618:beta-Lactamases / Q000032:analysis*
chemical_list: D001426:Bacterial Proteins; D001618:beta-Lactamases; C063912:carbapenemase
keywords: Carba NP test; Carbapenem; clinical sample