title: Ozone oxidative postconditioning ameliorates joint damage and decreases pro-inflammatory cytokine levels and oxidative stress in PG/PS-induced arthritis in rats.
abstract: Rheumatoid Arthritis (RA) is the most prevalent chronic condition present in ~1% of the adult population. Many pro-inflammatory mediators are increased in RA, including Reactive Oxygen Species such as nitric oxide NO, pro-inflammatory cytokines as tumor necrosis factor alpha (TNF-α), interleukin-1beta (IL-1β) and other molecules. Ozone oxidative postconditioning has regulatory effects on some pathological targets associated with RA. Thus, the aim of this study was to investigate the efficacy of ozone therapy in PG/PS-induced arthritis in rats in point of joints inflammation and morphology. Moreover, cytokines, nitric oxide and oxidative stress levels in spleen homogenates were evaluated. Ozone treatment ameliorated joint damage, reduced TNF-α concentrations as well as TNF-α and IL-1β mRNA levels. Besides, cellular redox balance, nitric oxide and fructolysine levels were reestablished after ozone oxidative postconditioning. It was concluded that pleiotropic ozone's effects clarify its therapeutic efficacy in RA. Decreasing inflammation and joint injury, reduction of pro-inflammatory cytokines, TNF-α and IL-1β transcripts and re-establishment of cellular redox balance after ozone treatment were demonstrated.
authors.affiliation: Pharmacy and Food Institute, University of Havana, Havana 10 400, Cuba.
authors.forename: Jaqueline Dranguet
authors.initials: JD
authors.lastname: Vaillant
authors.forename: Angela
authors.initials: A
authors.lastname: Fraga
authors.forename: María Teresa
authors.initials: MT
authors.lastname: Díaz
authors.forename: A
authors.initials: A
authors.lastname: Mallok
authors.forename: Renate
authors.initials: R
authors.lastname: Viebahn-Hänsler
authors.forename: Ziad
authors.initials: Z
authors.lastname: Fahmy
authors.forename: Ariana
authors.initials: A
authors.lastname: Barberá
authors.forename: Liván
authors.initials: L
authors.lastname: Delgado
authors.forename: Silvia
authors.initials: S
authors.lastname: Menéndez
authors.forename: Olga Sonia León
authors.initials: OS
authors.lastname: Fernández
mesh_terms: D000818:Animals; D001172:Arthritis, Rheumatoid / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism; D016207:Cytokines / Q000378:metabolism*; D005260:Female; D007249:Inflammation / Q000378:metabolism; D007596:Joints / Q000187:drug effects* / Q000378:metabolism; D010084:Oxidation-Reduction / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects*; D010126:Ozone / Q000494:pharmacology* / Q000627:therapeutic use; D010457:Peptidoglycan / Q000494:pharmacology*; D011134:Polysaccharides / Q000494:pharmacology*; D051381:Rats
chemical_list: D016207:Cytokines; D010457:Peptidoglycan; D011134:Polysaccharides; D010126:Ozone
keywords: Arthritis; Inflammation; Oxidative stress; Ozone oxidative postconditioning