title: One-stop genomic DNA extraction by salicylic acid-coated magnetic nanoparticles.
abstract: Salicylic acid-coated magnetic nanoparticles were prepared via a modified one-step synthesis and used for a one-stop extraction of genomic DNA from mammalian cells. The synthesized magnetic particles were used for magnetic separation of cells from the media by nonspecific binding of the particles as well as extraction of genomic DNA from the lysate. The quantity and quality were confirmed by agarose gel electrophoresis and polymerase chain reaction. The entire process of extraction and isolation can be completed within 30 min. Compared with traditional methods based on centrifugation and filtration, the established method is fast, simple, reliable, and environmentally friendly.
authors.affiliation: Bindley Bioscience Center, Purdue University, West Lafayette, IN 47906, USA; Birck Nanotechnology Center, Purdue University, West Lafayette, IN 47906, USA.
authors.forename: Zhongwu
authors.initials: Z
authors.lastname: Zhou
authors.forename: Ulhas S
authors.initials: US
authors.lastname: Kadam
authors.forename: Joseph
authors.initials: J
authors.lastname: Irudayaraj
mesh_terms: D005591:Chemical Fractionation / Q000379:methods*; D004247:DNA / Q000235:genetics / Q000302:isolation & purification*; D016678:Genome / Q000235:genetics*; D006367:HeLa Cells; D006801:Humans; D059346:Magnets / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry*; D020156:Salicylic Acid / Q000737:chemistry*; D013997:Time Factors
chemical_list: D004247:DNA; D020156:Salicylic Acid
keywords: Extraction; Genomic DNA; Magnetic nanoparticles; Salicylic acid; Simple and rapid
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