title: The transience of transient overexpression.
abstract: Much of what is known about mammalian cell regulation has been achieved with the aid of transiently transfected cells. However, overexpression can violate balanced gene dosage, affecting protein folding, complex assembly and downstream regulation. To avoid these problems, genome engineering technologies now enable the generation of stable cell lines expressing modified proteins at (almost) native levels.
authors.affiliation: Structural and Computational Biology Unit, European Molecular Biology Laboratory, Heidelberg, Germany. toby.gibson@embl.de
authors.forename: Toby J
authors.initials: TJ
authors.lastname: Gibson
authors.forename: Markus
authors.initials: M
authors.lastname: Seiler
authors.forename: Reiner A
authors.initials: RA
authors.lastname: Veitia
mesh_terms: D000818:Animals; D002460:Cell Line; D018628:Gene Dosage; D005786:Gene Expression Regulation; D016678:Genome; D006801:Humans; D008958:Models, Molecular; D009154:Mutation; D015202:Protein Engineering / Q000379:methods; D015398:Signal Transduction / Q000235:genetics; D014162:Transfection / Q000379:methods*
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