title: Molecular cloning, genomic structure, and tissue distribution of EW135, a novel chicken egg white protein with group B scavenger receptor cysteine-rich domains.
abstract: Approximately 80 proteins are reported to be present in chicken egg white. The major function of egg white proteins isolated so far is to defend the egg yolk against infections. We recently isolated a novel protein termed EW135 from chicken egg white. In this paper, we have determined the complete amino acid sequence of EW135 based on cDNA cloning. EW135 consists of 970 amino acids with a putative signal peptide of 17 amino acids. It is composed exclusively of tandem repeats of nine group B scavenger receptor cysteine-rich (SRCR) domains separated by eight seven-amino acid peptides. The features of consensus sequences found in the group B SRCR domain were well conserved in EW135. The EW135 gene consists of putative 11 exons, with each SRCR domain being encoded by a single exon. Reverse transcription PCR showed that EW135 is expressed in only the oviduct among the 11 types of tissues tested. EW135 is a second soluble protein belonging to the group B SRCR domain superfamily identified in chickens. One of the important functions of proteins belonging to the group B SRCR domain superfamily is to recognize pathogens in innate immunity. It is, therefore, conceivable that EW135 could be involved in host defense in egg white.
authors.affiliation: Department of Applied Biochemistry, Tokai University, Hiratsuka, 259-1292, Japan.
authors.forename: Whayoung
authors.initials: W
authors.lastname: Yoo
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Nakamura
authors.forename: Hideki
authors.initials: H
authors.lastname: Asanuma
authors.forename: Misao
authors.initials: M
authors.lastname: Matsushita
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002645:Chickens; D003001:Cloning, Molecular; D016384:Consensus Sequence; D003545:Cysteine; D018076:DNA, Complementary / Q000235:genetics; D004527:Egg Proteins / Q000235:genetics / Q000378:metabolism*; D005091:Exons / Q000235:genetics; D005260:Female; D023281:Genomics; D007438:Introns / Q000235:genetics; D008969:Molecular Sequence Data; D010057:Oviducts / Q000166:cytology / Q000378:metabolism; D016133:Polymerase Chain Reaction; D017434:Protein Structure, Tertiary; D051122:Scavenger Receptors, Class B / Q000235:genetics / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D014018:Tissue Distribution
chemical_list: D018076:DNA, Complementary; D004527:Egg Proteins; D051122:Scavenger Receptors, Class B; D003545:Cysteine
references.citation: Proteomics. 2007 Oct;7(19):3558-68
references.pmid: 17722208
references.citation: J Virol. 2007 Jul;81(14):7371-9
references.pmid: 17494075
references.citation: Infect Immun. 1987 Oct;55(10):2509-17
references.pmid: 3115900
references.citation: Proc Natl Acad Sci U S A. 2006 Mar 14;103(11):4168-73
references.pmid: 16537503
references.citation: J Biol Chem. 1997 May 23;272(21):13743-9
references.pmid: 9153228
references.citation: J Immunol. 2011 Feb 15;186(4):2344-54
references.pmid: 21217009
references.citation: Biochem J. 1983 Apr 1;211(1):129-38
references.pmid: 6409085
references.citation: Blood. 2009 Jan 22;113(4):887-92
references.pmid: 18849484
references.citation: J Biol Chem. 2001 Mar 23;276(12):9400-5
references.pmid: 11136738
references.citation: Pharmacol Rev. 2011 Dec;63(4):967-1000
references.pmid: 21880988
references.citation: BMC Evol Biol. 2010 Jun 15;10:181
references.pmid: 20550670
references.citation: Curr Pharm Des. 2007;13(9):875-84
references.pmid: 17430187
references.citation: J Biol Chem. 2004 Dec 3;279(49):51561-7
references.pmid: 15448162
references.citation: Trends Biochem Sci. 1994 Jan;19(1):5-8
references.pmid: 8140623
references.citation: Immunogenetics. 2002 Dec;54(9):621-34
references.pmid: 12466895
references.citation: Eur J Biochem. 1998 Dec 15;258(3):1022-31
references.pmid: 9990321
references.citation: J Immunol. 1998 May 15;160(10):4943-50
references.pmid: 9590242
references.citation: Crit Rev Immunol. 2004;24(1):1-37
references.pmid: 14995912
references.citation: Biosci Biotechnol Biochem. 1996 Sep;60(9):1505-6
references.pmid: 8987604
references.citation: Biochemistry. 1987 Jan 13;26(1):193-201
references.pmid: 3548816
references.citation: Arch Biochem Biophys. 1966 Sep 9;115(3):536-44
references.pmid: 5970480
references.citation: Nature. 2001 Aug 23;412(6849):835-8
references.pmid: 11518970
references.citation: J Biol Chem. 2005 Oct 21;280(42):35391-8
references.pmid: 16030018
references.citation: Biochim Biophys Acta. 2012 Mar;1820(3):218-25
references.pmid: 21854833
references.citation: J Agric Food Chem. 2006 May 31;54(11):3901-10
references.pmid: 16719513
references.citation: J Exp Med. 1999 Jan 18;189(2):413-22
references.pmid: 9892623
references.citation: Nat Genet. 1997 Sep;17(1):32-9
references.pmid: 9288095
references.citation: J Biol Chem. 1997 Mar 7;272(10):6151-8
references.pmid: 9045627
references.citation: Comp Biochem Physiol B. 1991;100(1):1-9
references.pmid: 1756612
references.citation: Eur J Immunol. 1993 Sep;23(9):2320-5
references.pmid: 8370408