title: DNA conjugation to nanoparticles.
abstract: Nanoparticle-DNA (NP-DNA) conjugates have been highlighted due to their versatility in diverse science and engineering fields. The protocol of DNA conjugation to gold nanoparticles (AuNPs), which are among the most popular NPs in bio-applications, is thus described here. This protocol also includes ligand exchange of AuNP to make AuNPs suitable for conjugation process and a fluorescence technique to evaluate the average number of DNA strands attached to single AuNP.
authors.affiliation: Dankook University, Yongin-si, Gyeonggi-do, South Korea.
authors.forename: Sunho
authors.initials: S
authors.lastname: Park
mesh_terms: D001665:Binding Sites; D004247:DNA / Q000737:chemistry*; D015180:Electrophoresis, Gel, Two-Dimensional; D005453:Fluorescence; D005612:Freeze Drying / Q000379:methods; D006046:Gold / Q000737:chemistry*; D008024:Ligands; D053768:Metal Nanoparticles / Q000737:chemistry*; D036103:Nanotechnology / Q000379:methods; D013194:Staining and Labeling / Q000379:methods; D013499:Surface Properties
chemical_list: D008024:Ligands; D006046:Gold; D004247:DNA