title: Drug-to-antibody ratio (DAR) by UV/Vis spectroscopy.
abstract: UV/Vis spectroscopy is a simple and convenient method to determine protein concentrations as well as the average number of drugs that are conjugated to the antibody in an antibody-drug conjugate (ADC). Using the measured absorbances of the ADC and the extinction coefficients of the antibody and the drug, the average drug-to-antibody ratio (DAR) can be determined.
authors.affiliation: Protein Analytical Chemistry, Genentech Inc., South San Francisco, CA, USA.
authors.forename: Yan
authors.initials: Y
authors.lastname: Chen
mesh_terms: D000042:Absorption; D018796:Immunoconjugates / Q000032:analysis* / Q000737:chemistry*; D004364:Pharmaceutical Preparations / Q000032:analysis* / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet
chemical_list: D018796:Immunoconjugates; D004364:Pharmaceutical Preparations