title: [Using an antibiotic sensitivity assay on urine cultures from patients attending general practice healthcare institutions in Pereira].
abstract: OBJECTIVE
  Describing the bacteria most frequently isolated from and antibiotic resistance patterns in urine cultures taken from patients attending general practice healthcare institutions in Pereira as urinary tract infections are a frequent cause for primary healthcare attention.
  
  
  METHODS
  A descriptive cross-sectional observational study of 131,608 members of the health social security system was carried out between January 1st 2010 and April 7th 2011;the bacteria isolated from urine cultures having more than 105 colony-forming units (CFU) were assessed, as were the sensitivity and antibacterial agents' resistance results. Resistance was evaluated in line with Clinical and Laboratory Standards Institute (CLSI) parameters by means of the Kirby-Bauer disk diffusion manual method with Beckton Dickinson diagnostic disks.
  
  
  RESULTS
  5,226 urine cultures were analysed, 1,058 of which showed uropathogengrowth. Bacterial growth was reported in 792 cultures (74.9 %), having more than 105 CFU. The most frequently isolated microorganisms were Escherichia coli (67.2 %), Klebsiella sp (19.2 %) and Enterococcus sp (7.8 %).Escherichia colishowed high sensitivity to amoxicillin / clavulanate (100 %), nitrofurantoin (94.8 %), ceftriaxone (86.3 %), ciprofloxacin (71.0 %) and high resistance to ampicillin (54.7 %), amoxicillin (50.0 %), trimethoprim-sulfamethoxazole (43.8 %) and cephalothin (42.8 %).
  
  
  CONCLUSIONS
  Nitrofurantoin may be the best therapeutic option in first-line management of lower urinary tract infection and ciprofloxacin for pyelonephritis due to its effectiveness and low resistance as seen in primary healthcare practice in Pereira.
authors.affiliation: Departamento de Farmacoepidemiología, Audifarma S.A, Grupo de Investigación en Farmacoepidemiología y Farmacovigilancia, Facultad de Ciencias de la Salud, Universidad Tecnológica de Pereira, Colombia. machado@utp.edu.co
authors.forename: Jorge E
authors.initials: JE
authors.lastname: Machado-Alba
authors.forename: María M
authors.initials: MM
authors.lastname: Murillo-Muñoz
mesh_terms: D000900:Anti-Bacterial Agents; D003105:Colombia; D003430:Cross-Sectional Studies; D006268:Health Facilities; D006801:Humans; D008826:Microbial Sensitivity Tests; D011320:Primary Health Care; D014552:Urinary Tract Infections / Q000382:microbiology* / Q000652:urine*; D014556:Urine / Q000382:microbiology
chemical_list: D000900:Anti-Bacterial Agents