title: Fusarium oxysporum infection assays in Arabidopsis.
abstract: Increased susceptibility to Fusarium oxysporum is one of the most conspicuous characteristics of the Arabidopsis mutants lacking the heterotrimeric G protein β and γ1 subunits. The molecular mechanisms placing these G proteins in the plant innate immunity network are yet to be discovered. However, a method to test susceptibility to and disease progression of an important plant pathogen, such as F. oxysporum, is of central importance to many plant defense studies. The optimized protocol presented here allows the routine processing and analysis of symptom progression in young Arabidopsis soil-grown seedlings and yields highly reproducible results.
authors.affiliation: Plant Genetic Engineering Laboratory, School of Agriculture and Food Sciences, University of Queensland, Brisbane, QLD, Australia.
authors.forename: Yuri
authors.initials: Y
authors.lastname: Trusov
authors.forename: David
authors.initials: D
authors.lastname: Chakravorty
authors.forename: Jose Ramon
authors.initials: JR
authors.lastname: Botella
mesh_terms: D017360:Arabidopsis / Q000235:genetics* / Q000382:microbiology; D018450:Disease Progression; D004198:Disease Susceptibility; D060585:Fusariosis / Q000235:genetics / Q000382:microbiology; D005670:Fusarium / Q000472:pathogenicity; D018506:Gene Expression Regulation, Plant; D020962:Heterotrimeric GTP-Binding Proteins / Q000235:genetics*; D009154:Mutation; D010935:Plant Diseases / Q000235:genetics*; D021122:Protein Subunits / Q000235:genetics; D015398:Signal Transduction; D012988:Soil Microbiology
chemical_list: D021122:Protein Subunits; D020962:Heterotrimeric GTP-Binding Proteins