title: Polypeptide/doxorubicin hydrochloride polymersomes prepared through organic solvent-free technique as a smart drug delivery platform.
abstract: Rapid and efficient side-chain functionalization of polypeptide with neighboring carboxylgroups is achieved via the combination of ring-opening polymerization and subsequent thiol-yne click chemistry. The spontaneous formation of polymersomes with uniform size is found to occur in aqueous medium via electrostatic interaction between the anionic polypeptide and cationic doxorubicin hydrochloride (DOX·HCl). The polymersomes are taken up by A549 cells via endocytosis, with a slightly lower cytotoxicity compared with free DOX ·HCl. Moreover, the drug-loaded polymersomes exhibit the enhanced therapeutic efficacy, increase apoptosis in tumor tissues, and reduce systemic toxicity in nude mice bearing A549 lung cancer xenograft, in comparison with free DOX ·HCl.
authors.affiliation: Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, P. R. China; University of Chinese Academy of Sciences, Beijing, 100039, P. R. China.
authors.forename: Mingqiang
authors.initials: M
authors.lastname: Li
authors.forename: Shixian
authors.initials: S
authors.lastname: Lv
authors.forename: Zhaohui
authors.initials: Z
authors.lastname: Tang
authors.forename: Wantong
authors.initials: W
authors.lastname: Song
authors.forename: Haiyang
authors.initials: H
authors.lastname: Yu
authors.forename: Hai
authors.initials: H
authors.lastname: Sun
authors.forename: Huaiyu
authors.initials: H
authors.lastname: Liu
authors.forename: Xuesi
authors.initials: X
authors.lastname: Chen
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D045744:Cell Line, Tumor; D004317:Doxorubicin / Q000494:pharmacology*; D016503:Drug Delivery Systems / Q000379:methods*; D004705:Endocytosis / Q000187:drug effects; D005434:Flow Cytometry; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D057446:Hydrodynamics; D006863:Hydrogen-Ion Concentration / Q000187:drug effects; D007150:Immunohistochemistry; D042541:Intracellular Space / Q000187:drug effects / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D051379:Mice; D008819:Mice, Nude; D008856:Microscopy, Fluorescence; D009930:Organic Chemicals / Q000737:chemistry*; D010455:Peptides / Q000494:pharmacology*; D011092:Polyethylene Glycols / Q000737:chemistry; D011108:Polymers / Q000138:chemical synthesis* / Q000737:chemistry; D011817:Rabbits; D012997:Solvents; D013997:Time Factors; D016896:Treatment Outcome
chemical_list: D009930:Organic Chemicals; D010455:Peptides; D011108:Polymers; D012997:Solvents; D011092:Polyethylene Glycols; D004317:Doxorubicin; C028210:monomethoxypolyethylene glycol
keywords: drug delivery system; pH-responsive; polypeptide; self-assembly; thiol-yne photochemistry