title: Ethanol alters gene expression and cell organization during optic vesicle evagination.
abstract: Ethanol has been described as a teratogen in vertebrate development. During early stages of brain formation, ethanol affects the evagination of the optic vesicles, resulting in synophthalmia or cyclopia, phenotypes where the optic vesicles partially or totally fuse. The mechanisms by which ethanol affects the morphogenesis of the optic vesicles are however largely unknown. In this study we make use of in situ hybridization, electron microscopy and immunohistochemistry to show that ethanol has profound effects on cell organization and gene expression during the evagination of the optic vesicles. Exposure to ethanol during early eye development alters the expression patterns of some genes known to be important for eye morphogenesis, such as rx3/1 and six3a. Furthermore, exposure to ethanol interferes with the acquisition of neuroepithelial features by the eye field cells, which is clear at ultrastructual level. Indeed, ethanol disrupts the acquisition of fusiform cellular shapes within the eye field. In addition, tight junctions do not form and retinal progenitors do not properly polarize, as suggested by the mis-localization and down-regulation of zo1. We also show that the ethanol-induced cyclopic phenotype is significantly different to that observed in cyclopic mutants, suggesting a complex effect of ethanol on a variety of targets. Our results show that ethanol not only disrupts the expression pattern of genes involved in retinal morphogenesis, such as rx3 and rx1, but also disrupts the changes in cell polarity that normally occur during eye field splitting. Thus, ethylic teratology seems to be related not only to modifications in gene expression and cell death but also to alterations in cell morphology.
authors.affiliation: Departamento de Biología Celular y Patología, IBSAL-Instituto de Neurociencias de Castilla y León, Universidad de Salamanca, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Santos-Ledo
authors.forename: F
authors.initials: F
authors.lastname: Cavodeassi
authors.forename: H
authors.initials: H
authors.lastname: Carreño
authors.forename: J
authors.initials: J
authors.lastname: Aijón
authors.forename: R
authors.initials: R
authors.lastname: Arévalo
mesh_terms: D000818:Animals; D016923:Cell Death; D002492:Central Nervous System Depressants / Q000494:pharmacology*; D004625:Embryo, Nonmammalian; D000431:Ethanol / Q000494:pharmacology*; D005123:Eye / Q000187:drug effects / Q000254:growth & development*; D005136:Eye Proteins / Q000096:biosynthesis* / Q000235:genetics; D015870:Gene Expression / Q000187:drug effects*; D018507:Gene Expression Regulation, Developmental; D007091:Image Processing, Computer-Assisted; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D020287:In Situ Nick-End Labeling; D008854:Microscopy, Electron; D009024:Morphogenesis; D009154:Mutation / Q000502:physiology; D010641:Phenotype; D060888:Real-Time Polymerase Chain Reaction; D019108:Tight Junctions / Q000235:genetics / Q000502:physiology; D014794:Visual Fields / Q000502:physiology; D015027:Zebrafish; D062826:Zonula Occludens-1 Protein / Q000096:biosynthesis / Q000235:genetics
chemical_list: D002492:Central Nervous System Depressants; D005136:Eye Proteins; D062826:Zonula Occludens-1 Protein; D000431:Ethanol
keywords: ISH; MET; MHB; SEM; TUNEL; ZO-1; cell polarity; cyclopic mutants; eye specification; hours post-fertilization; hpf; in situ hybridization; mesenchymal–epithelial transition; midbrain–hindbrain boundary; morphogenesis; oep; one-eye pinhead; qRT-PCR; quantitative real-time polymerase chain reaction; somites stage; ss; standard error of mean; terminal deoxynucleotidyl transferase dUTP nick end labeling; zonula-occludens-1
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