title: Simultaneous determination of methocarbamol and Ibuprofen by first derivative synchronous fluorescence spectroscopic method in their binary mixture and spiked human plasma.
abstract: Methocarbamol is formulated with Ibuprofen for treatment of alleviated pain associated with muscle spasm. This manuscript describes a sensitive and selective first derivative synchronous spectrofluorimetric method for simultaneous determination of both drugs. Factors affecting method selectivity were studied where best results were obtained upon using Δ λ = 20 and water as a solvent. Methocartbamol was determined at 283 nm while Ibuprofen at 285.5 nm in the concentration ranges of 0.4-5 and 0.2-4.8 μg/mL, respectively. The applicability of the proposed method was ascertained by application to different laboratory prepared mixtures and marketed formulation. The high sensitivity achieved by the proposed method permitted its application for determination of the drugs in human plasma spiked with pure drugs and their combined tablets. The proposed method showed no significant difference when compared with the reported HPLC method using student's t-test and F-ratio test.
authors.affiliation: Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Bani-Suef University, Alshaheed Shehata Ahmad Hegazy St, 62111, Beni-Suef, Egypt, nadasayed2003@yahoo.com.
authors.forename: Nada S
authors.initials: NS
authors.lastname: Abdelwahab
authors.forename: Maha M
authors.initials: MM
authors.lastname: Abdelrahman
mesh_terms: D006801:Humans; D007052:Ibuprofen / Q000097:blood*; D008721:Methocarbamol / Q000097:blood*; D013050:Spectrometry, Fluorescence
chemical_list: D008721:Methocarbamol; D007052:Ibuprofen
references.citation: Talanta. 2009 Dec 15;80(2):880-8
references.pmid: 19836568
references.citation: Chem Cent J. 2011 Nov 07;5(1):70
references.pmid: 22059735
references.citation: Acta Pharm. 2012 Jun;62(2):191-200
references.pmid: 22750817
references.citation: Int J Biomed Sci. 2009 Jun;5(2):136-45
references.pmid: 23675128
references.citation: J Fluoresc. 2009 Sep;19(5):817-28
references.pmid: 19408106