title: Dietary glutamine supplementation prevents mucosal injury and modulates intestinal epithelial restitution following acetic acid induced intestinal injury in rats.
abstract: Beneficial effects of glutamine (GLN) have been described in many gastrointestinal disorders. The aim of the present study was to evaluate the preventative effect of oral GLN supplementation against acetic acid (AA) induced intestinal injury in a rat. Male Sprague-Dawley rats were divided into four experimental groups: control (CONTR) rats underwent laparotomy, control-glutamine (CONTR-GLN) rats were treated with enteral glutamine given in drinking water (2%) 48 hours before and five days following laparotomy, AA rats underwent laparotomy and injection of AA into an isolated jejunal loop, and acetic acid-glutamine (AA-GLN) rats underwent AA-induced injury and were treated with enteral GLN 48 hours before and 5 days following laparotomy. Intestinal mucosal damage (Park's injury score), mucosal structural changes, enterocyte proliferation and enterocyte apoptosis were determined five days following intestinal injury. Western blotting was used to determine p-ERK and bax protein levels. AA-induced intestinal injury resulted in a significantly increased intestinal injury score with concomitant inhibition of cell turnover (reduced proliferation and enhanced apoptosis). Treatment with dietary GLN supplementation resulted in a decreased intestinal injury score with concomitant stimulation of cell turnover (enhanced proliferation and reduced apoptosis). In conclusion, pre-treatment with oral GLN prevents mucosal injury and improves intestinal recovery following AA-induced intestinal injury in rats.
authors.affiliation: Department of Surgery, Bnai Zion Medical Center, Haifa, Israel.
authors.forename: Forat
authors.initials: F
authors.lastname: Swaid
authors.affiliation: Laboratory of Intestinal Adaptation and Recovery, The Bruce Rappaport Faculty of Medicine, Technion-Israel Institute of Technology, Haifa, Israel.|Department Pediatric Surgery, Bnai Zion Medical Center, 47 Golomb St., POB 4940, Haifa 31048, Israel.
authors.forename: Igor
authors.initials: I
authors.lastname: Sukhotnik
authors.affiliation: Department of Surgery, Bnai Zion Medical Center, Haifa, Israel.
authors.forename: Ibrahim
authors.initials: I
authors.lastname: Matter
authors.affiliation: Department of Gastroenterology, Bnai Zion Medical Center, Haifa, Israel.
authors.forename: Drora
authors.initials: D
authors.lastname: Berkowitz
authors.affiliation: Elderly Care Department, Queen Elizabeth II Hospital, Welwyn Garden City, United Kingdom.
authors.forename: Christopher
authors.initials: C
authors.lastname: Hadjittofi
authors.affiliation: Laboratory of Intestinal Adaptation and Recovery, The Bruce Rappaport Faculty of Medicine, Technion-Israel Institute of Technology, Haifa, Israel.
authors.forename: Yulia
authors.initials: Y
authors.lastname: Pollak
authors.affiliation: Department of Gastroenterology, Bnai Zion Medical Center, Haifa, Israel.
authors.forename: Alexandra
authors.initials: A
authors.lastname: Lavy
keywords: Acetic acid; Colitis; Crohn’s; Enterocyte apoptosis; Enterocyte proliferation; Glutamine; Inflammation; Intestine
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