title: Determination of the carbohydrates from Notopterygium forbesii Boiss by HPLC with fluorescence detection.
abstract: A sensitive pre-column derivatization method was developed for analysis of carbohydrates by HPLC with fluorescence detection. The introduction of 2-(12-benzo[b]acridin-5(12H)-yl)-acetohydrazide (BAAH) with excellent fluorescence property into the molecules of monosaccharides greatly enhanced the HPLC sensitivity of the analytes. Meanwhile, derivatization with BAAH also greatly increased the hydrophobicity of the monosaccharides and made them elute at increased retention times. The monosaccharides with similar properties therefore could be completely separated due to the increased interaction between the analytes and the column. Component monosaccharides of the polysaccharides obtained from the roots, stems and leaves of Notopterygium forbesii Boiss (NF) were analyzed by the developed method. The results indicated that the polysaccharides of NF were mainly composed of d-galactose and d-glucose. This is the first systematic study of the sugar composition of the polysaccharides of NF. It will be helpful for the quality control of NF.
authors.affiliation: Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of Science, Xining, PR China.
authors.forename: Shijuan
authors.initials: S
authors.lastname: Zhang
authors.forename: Chunli
authors.initials: C
authors.lastname: Li
authors.forename: Guoying
authors.initials: G
authors.lastname: Zhou
authors.forename: Guodong
authors.initials: G
authors.lastname: Che
authors.forename: Jinmao
authors.initials: J
authors.lastname: You
authors.forename: Yourui
authors.initials: Y
authors.lastname: Suo
mesh_terms: D019342:Acetic Acid / Q000737:chemistry; D019661:Apiaceae / Q000737:chemistry*; D002241:Carbohydrates / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005453:Fluorescence; D006835:Hydrazones / Q000737:chemistry; D057230:Limit of Detection; D009005:Monosaccharides / Q000737:chemistry; D013696:Temperature
chemical_list: D002241:Carbohydrates; D006835:Hydrazones; D009005:Monosaccharides; D019342:Acetic Acid
keywords: Fluorescence detection; HPLC; Monosaccharides; Notopterygium forbesii Boiss; Polysaccharides