title: Casein kinase 2 is essential for mitophagy.
abstract: Mitophagy is a process that selectively degrades mitochondria. When mitophagy is induced in yeast, the mitochondrial outer membrane protein Atg32 is phosphorylated, interacts with the adaptor protein Atg11 and is recruited into the vacuole with mitochondria. We screened kinase-deleted yeast strains and found that CK2 is essential for Atg32 phosphorylation, Atg32-Atg11 interaction and mitophagy. Inhibition of CK2 specifically blocks mitophagy, but not macroautophagy, pexophagy or the Cvt pathway. In vitro, CK2 phosphorylates Atg32 at serine 114 and serine 119. We conclude that CK2 regulates mitophagy by directly phosphorylating Atg32.
authors.affiliation: Laboratory of Biosignaling, Niigata University Graduate School of Medical and Dental Sciences, Niigata 951-8510.
authors.forename: Tomotake
authors.initials: T
authors.lastname: Kanki
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Kurihara
authors.forename: Xiulian
authors.initials: X
authors.lastname: Jin
authors.forename: Tadahiro
authors.initials: T
authors.lastname: Goda
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Ono
authors.forename: Masamune
authors.initials: M
authors.lastname: Aihara
authors.forename: Yuko
authors.initials: Y
authors.lastname: Hirota
authors.forename: Tetsu
authors.initials: T
authors.lastname: Saigusa
authors.forename: Yoshimasa
authors.initials: Y
authors.lastname: Aoki
authors.forename: Takeshi
authors.initials: T
authors.lastname: Uchiumi
authors.forename: Dongchon
authors.initials: D
authors.lastname: Kang
mesh_terms: D000071183:Autophagy-Related Proteins; D047390:Casein Kinase II / Q000235:genetics / Q000378:metabolism*; D008928:Mitochondria / Q000378:metabolism; D063306:Mitophagy; D018160:Receptors, Cytoplasmic and Nuclear / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism; D014617:Vacuoles / Q000378:metabolism; D033921:Vesicular Transport Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: C502923:Atg11 protein, S cerevisiae; C543183:Atg32 protein, S cerevisiae; D000071183:Autophagy-Related Proteins; D018160:Receptors, Cytoplasmic and Nuclear; D029701:Saccharomyces cerevisiae Proteins; D033921:Vesicular Transport Proteins; D047390:Casein Kinase II
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