title: N-containing functional groups induced superior cytocompatible and hemocompatible graphene by NH₂ ion implantation.
abstract: Graphene is functionalized with amine by NH2 ion implantation at room temperature in vacuum. The reaction is featured by nucleophilic substitution of C-O groups by the ammonia radicals. The presence of N-containing functional groups in graphene is identified by Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy. N element was successfully introduced to graphene, the atomic ratio of N to C rose to 3.12 %. NH2 ion implanted graphene (G-NH2) is a better hydrophilic material than pristine grahene according to the contact angle experiment. Mouse fibroblast cells and human endothelial cells cultured on G-NH2 displayed superior cell-viability, proliferation and stretching over that on pristine graphene. Platelet adhesion, hemolysis and Kinetic-clotting time were measured on G-NH2, showing excellent anticoagulation, with as good hemolysis as pristine graphene.
authors.affiliation: College of Physics and Electronic Information Science, Tianjin Normal University, Tianjin, 300387, China.
authors.forename: Meixian
authors.initials: M
authors.lastname: Guo
authors.forename: Minsi
authors.initials: M
authors.lastname: Li
authors.forename: Xiaoqi
authors.initials: X
authors.lastname: Liu
authors.forename: Mengli
authors.initials: M
authors.lastname: Zhao
authors.forename: Dejun
authors.initials: D
authors.lastname: Li
authors.forename: Dongsheng
authors.initials: D
authors.lastname: Geng
authors.forename: Xueliang
authors.initials: X
authors.lastname: Sun
authors.forename: Hanqing
authors.initials: H
authors.lastname: Gu
mesh_terms: D000588:Amines / Q000737:chemistry*; D000818:Animals; D000925:Anticoagulants / Q000737:chemistry; D001672:Biocompatible Materials / Q000737:chemistry; D001777:Blood Coagulation / Q000187:drug effects; D002448:Cell Adhesion; D049109:Cell Proliferation; D002470:Cell Survival; D042783:Endothelial Cells / Q000187:drug effects; D005347:Fibroblasts / Q000187:drug effects; D006108:Graphite / Q000737:chemistry*; D006461:Hemolysis; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D007477:Ions; D007700:Kinetics; D051379:Mice; D009584:Nitrogen / Q000737:chemistry*; D010973:Platelet Adhesiveness; D011817:Rabbits
chemical_list: D000588:Amines; D000925:Anticoagulants; D001672:Biocompatible Materials; D007477:Ions; D006108:Graphite; D009584:Nitrogen
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