title: Effects of administration of subtoxic doses of acetaminophen on liver and blood levels of insulin-like growth factor-1 in rats.
abstract: Acetaminophen (APAP) is widely used in the treatment of pain. Toxic doses of APAP cause acute liver failure, but therapeutic doses are believed to be safe. The purpose of this study is to investigate the effects of administration of subtoxic doses of APAP on liver and blood levels of insulin-like growth factor-1 (IGF-1) in rats. Low dose (100 mg/kg) and high dose (250 mg/kg) of APAP were intraperitoneally injected into Wistar albino rats. Following administration of therapeutic doses of APAP, there were no significant changes in serum transaminases and liver glutathione levels. Both doses of APAP induced a decrease in liver and blood levels of IGF-1 when compared with the controls. There was no significant difference in liver IGF-1 levels between the high-dose and low-dose APAP groups; however, there was a significant difference in blood IGF-1 levels between both the groups. The histological examination showed that low dose of APAP induced mild degree of structural change, while high dose of APAP induced severe structural damage. In conclusion, these results suggest that blood IGF-1 levels may have a value in predicting hepatic damage resulting from therapeutic doses of APAP.
authors.affiliation: Department of Pediatrics, Faculty of Medicine, Dokuz Eylul University, Izmir, Turkey.
authors.forename: Durgul
authors.initials: D
authors.lastname: Ozdemir
authors.affiliation: Department of Physiology, Faculty of Medicine, Dokuz Eylul University, Izmir, Turkey.
authors.forename: Ilkay
authors.initials: I
authors.lastname: Aksu
authors.affiliation: Department of Histology and Embriyology, High School of Physical Therapy and Rehabilitation, Dokuz Eylul University, Izmir, Turkey.
authors.forename: Basak
authors.initials: B
authors.lastname: Baykara
authors.affiliation: Department of Pharmacology, Faculty of Medicine, Vocational School of Health Services, Dokuz Eylul University, Izmir, Turkey.
authors.forename: Mehmet
authors.initials: M
authors.lastname: Ates
authors.affiliation: Department of Biochemistry, Faculty of Medicine, Dokuz Eylul University, Izmir, Turkey.
authors.forename: Ali Riza
authors.initials: AR
authors.lastname: Sisman
authors.affiliation: Department of Physiology, Faculty of Medicine, Dokuz Eylul University, Izmir, Turkey.
authors.forename: Muge
authors.initials: M
authors.lastname: Kiray
authors.affiliation: Department of Pathology, St Luke's-Roosevelt Hospital, New York, NY, USA College of Physicians and Surgeons, Columbia University, New York, NY, USA.
authors.forename: Arzu
authors.initials: A
authors.lastname: Buyuk
authors.affiliation: Department of Physiology, Faculty of Medicine, Dokuz Eylul University, Izmir, Turkey nazan.uysal@deu.edu.tr.
authors.forename: Nazan
authors.initials: N
authors.lastname: Uysal
mesh_terms: D000082:Acetaminophen / Q000008:administration & dosage / Q000494:pharmacology*; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D056486:Chemical and Drug Induced Liver Injury / Q000473:pathology; D004305:Dose-Response Relationship, Drug; D005978:Glutathione / Q000378:metabolism; D007334:Insulin-Like Growth Factor I / Q000378:metabolism*; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000975:Antioxidants; C548904:insulin-like growth factor-1, rat; D000082:Acetaminophen; D007334:Insulin-Like Growth Factor I; D005978:Glutathione
keywords: APAP; IGF-1; apoptosis; glutathione; liver damage