title: [Study on ultrasonic extraction and macroporous resin purification techniques of total flavones from Sedum lineare].
abstract: OBJECTIVE
  To optimize the ultrasonic extraction condition and purification process of total flavonoids in Sedum lineare by macroporous resin.
  
  
  METHODS
  Optimum ultrasonic extraction conditions (ethanol concentration, solid/liquid, ultrasonic time, working temperature) were obtained through orthogonal design. The adsorption ratio and eluting ratio of total flavones were selected as indexes.
  
  
  RESULTS
  The optimum ultrasonic extraction condition was 80% ethanol, solid/liquid 1:40, ultrasonic 20 min at 30 degrees C. The adsorbed AB-8 macroporous resin column was eluted by 80% ethanol at the eluting velocity of 2 mL/min, the eluting ratio and purity of total flavones was 94.72% and 43.51%, respectively.
  
  
  CONCLUSIONS
  The method is simple and low cost.
authors.affiliation: School of Pharmaceutical Sciences, Southern Medical University, Guangzhou 510515, China. sujianwei-202@163.com
authors.forename: Jian-Wei
authors.initials: JW
authors.lastname: Su
authors.forename: Zhi-Min
authors.initials: ZM
authors.lastname: Jia
authors.forename: Yang
authors.initials: Y
authors.lastname: Xie
mesh_terms: D000327:Adsorption; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification*; D000431:Ethanol / Q000737:chemistry; D047309:Flavones / Q000737:chemistry / Q000302:isolation & purification*; D015203:Reproducibility of Results; D012117:Resins, Synthetic / Q000737:chemistry*; D031963:Sedum / Q000737:chemistry*; D012995:Solubility; D013678:Technology, Pharmaceutical / Q000379:methods; D013696:Temperature; D013997:Time Factors; D014465:Ultrasonics
chemical_list: D004365:Drugs, Chinese Herbal; D047309:Flavones; D012117:Resins, Synthetic; D000431:Ethanol