title: New cytotoxic triterpenoid saponins from the whole plant of Clematis lasiandra Maxim.
abstract: Four new oleanane type triterpenoid saponins (1-4) and three known saponins (5-7) were isolated from the whole plant of Clematis lasiandra Maxim. The structures of the four new compounds were elucidated as 3-O-β-D-ribopyranosyl-(1 → 3)-α-L-rhamnopyranosyl-(1 → 2)-[β-D-glucopyranosyl-(1 → 4)]-β-D-xylopyranosyl hederagenin (1), 3-O-β-D-ribopyranosyl-(1 → 3)-α-L-rhamnopyranosyl-(1→2)-β-D-xylopyranosyl oleanolic acid 28-O-β-D-glucopyranosyl ester (2), 3-O-β-D-ribopyranosyl-(1 → 3)-α-L-rhamnopyranosyl-(1 → 2)-β-D-xylopyranosyl hederagenin (3) and 3-O-β-D-ribopyranosyl-(1 → 3)-α-L-rhamnopyranosyl-(1 → 2)-[β-D-glucopyranosyl-(1→4)]-α-L-arabinopyranosyl hederagenin (4) on the basis of extensive spectroscopic analysis and chemical evidence. Compounds 1-7 were evaluated for their cytotoxicity against human tumor cell lines HL-60, Hep-G2 and SGC-7901, and all of the evaluated saponins showed significant cytotoxicity to those three tumor cell lines with IC₅₀ in the range from 1.40 to 19.50 μmol/L except for compounds 2 and 6.
authors.affiliation: Research & Development Center of Biorational Pesticide, College of Plant Protection, Northwest A&F University, Yangling 712100, PR China.
authors.forename: Xiangrong
authors.initials: X
authors.lastname: Tian
authors.forename: Juntao
authors.initials: J
authors.lastname: Feng
authors.forename: Haifeng
authors.initials: H
authors.lastname: Tang
authors.forename: Mei
authors.initials: M
authors.lastname: Zhao
authors.forename: Yuan
authors.initials: Y
authors.lastname: Li
authors.forename: Wenli
authors.initials: W
authors.lastname: Hai
authors.forename: Xing
authors.initials: X
authors.lastname: Zhang
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D039324:Clematis / Q000737:chemistry*; D018922:HL-60 Cells; D056945:Hep G2 Cells; D006801:Humans; D015394:Molecular Structure; D009369:Neoplasms / Q000188:drug therapy*; D008517:Phytotherapy; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use*; D012503:Saponins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D014315:Triterpenes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D010936:Plant Extracts; D012503:Saponins; D014315:Triterpenes
keywords: Clematis lasiandra; Cytotoxicity; Ranunculaceae; Triterpenoid saponin