title: [Isolation and preliminary identification of stem-like cells in pituitary adenoma].
abstract: OBJECTIVE
  To isolate and identify the pituitary adenoma stem-like cells from pituitary adenoma tissue.
  
  
  METHODS
  Total RNA was prepared with 42 cases of pituitary adenoma tissue samples frozen in liquid nitrogen, the expression of Nestin was detected by RT-PCR. Tumor spheres were cultured in serum-free conditions and the immunefluorescence were used to detect the expression of stem cell markers such as Nestin, Sox2.
  
  
  RESULTS
  The positive rate of Nestin mRNA expression was 88.1% (37/42). The cultured tumor spheres were found positive for Nestin, Sox2 by immunefluorescence detection.
  
  
  CONCLUSIONS
  Stem cell markers are expressed in the pituitary adenoma tissue and the pituitary adenoma stem-like cells can be cultured in serum-free condition.
authors.affiliation: Department of Neurosurgery, West China Hospital, Sichuan University, Chengdu 610041, China.
authors.forename: Pei-Zhi
authors.initials: PZ
authors.lastname: Zhou
authors.forename: Wei-Chao
authors.initials: WC
authors.lastname: Ma
authors.forename: Xiu-Jie
authors.initials: XJ
authors.lastname: Wang
authors.forename: Shu-Wen
authors.initials: SW
authors.lastname: Cheng
authors.forename: Shu
authors.initials: S
authors.lastname: Jiang
mesh_terms: D000236:Adenoma / Q000378:metabolism / Q000473:pathology*; D000328:Adult; D000368:Aged; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D014411:Neoplastic Stem Cells / Q000166:cytology*; D064231:Nestin / Q000235:genetics / Q000378:metabolism; D010911:Pituitary Neoplasms / Q000378:metabolism / Q000473:pathology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D055748:SOXB1 Transcription Factors / Q000235:genetics / Q000378:metabolism
chemical_list: C577484:NES protein, human; D064231:Nestin; D012333:RNA, Messenger; C527782:SOX2 protein, human; D055748:SOXB1 Transcription Factors