title: Production and application of an enzyme blend from Chrysoporthe cubensis and Penicillium pinophilum with potential for hydrolysis of sugarcane bagasse.
abstract: Blending of the enzyme extracts produced by different fungi can result in favorable synergetic enhancement of the enzyme blend with regards to the main cellulase activities, as well as the inclusion of accessory enzymes that may not be as abundant in enzyme extracts produced by predominantly cellulase producing fungi. The Chrysoporthe cubensis:Penicillium pinophilum 50:50 (v/v) blend produced herein presented good synergy, especially for FPase and endoglucanase activities which were 76% and 48% greater than theoretical, respectively. This enzyme blend was applied to sugarcane bagasse previously submitted to a simple alkali pretreatment. Glucan hydrolysis efficiency reached an excess of 60% and xylan conversion exceeded 90%. Increasing the hydrolysis temperature from 45 to 50°C also resulted in a 16-20% increase in conversion of both glucan and xylan fractions. The blended enzyme extract obtained therefore showed great potential for application in the lignocellulose hydrolysis process.
authors.affiliation: Departamento de Bioquímica e Biologia Molecular, Universidade Federal de Viçosa, Campus Universitário, Viçosa, MG, Brazil. evanvisser@gmail.com
authors.forename: Evan Michael
authors.initials: EM
authors.lastname: Visser
authors.forename: Daniel Luciano
authors.initials: DL
authors.lastname: Falkoski
authors.forename: Maíra Nicolau
authors.initials: MN
authors.lastname: de Almeida
authors.forename: Gabriela Piccolo
authors.initials: GP
authors.lastname: Maitan-Alfenas
authors.forename: Valéria Monteze
authors.initials: VM
authors.lastname: Guimarães
mesh_terms: D001203:Ascomycota / Q000201:enzymology*; D018533:Biomass; D001709:Biotechnology / Q000379:methods*; D050260:Carbohydrate Metabolism; D002482:Cellulose / Q000378:metabolism*; D005656:Fungal Proteins / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D006868:Hydrolysis; D007700:Kinetics; D010407:Penicillium / Q000201:enzymology*; D031786:Saccharum / Q000378:metabolism*; D013696:Temperature; D014994:Xylose / Q000378:metabolism
chemical_list: D005656:Fungal Proteins; D002482:Cellulose; C027433:bagasse; D014994:Xylose; D005947:Glucose
keywords: Chrysoporthe cubensis; Penicillium pinophilum; Saccharification; Sugarcane bagasse; Synergy