title: Methods for conjugating antibodies to nanocarriers.
abstract: Antibodies are one of the most commonly used targeting ligands for nanocarriers, mainly because they are specific, have a strong binding affinity, and are available for a number of disease biomarkers. The bioconjugation chemistry can be a crucial factor in determining the targeting efficiency of drug delivery and should be chosen on a case-by-case basis. An antibody consists of a number of functional groups which offer many flexible options for bioconjugation. This chapter focuses on discussing some of the approaches including periodate oxidation, carbodiimide, maleimide, and heterofunctional linkers, for conjugating antibodies to different nanocarriers. The advantages and limitations are described herein. Specific examples are selected to demonstrate the experimental procedures and to illustrate the potential for applying to other nanocarrier system.
authors.affiliation: Department of Pharmaceutical Sciences, College of Pharmacy, Nursing and Allied Sciences, North Dakota State University, Fargo, ND, USA.
authors.forename: Anil
authors.initials: A
authors.lastname: Wagh
authors.forename: Benedict
authors.initials: B
authors.lastname: Law
mesh_terms: D000588:Amines / Q000737:chemistry; D000906:Antibodies / Q000737:chemistry*; D002234:Carbodiimides / Q000737:chemistry; D002241:Carbohydrates / Q000737:chemistry; D002264:Carboxylic Acids / Q000737:chemistry; D004337:Drug Carriers / Q000737:chemistry*; D006834:Hydrazines / Q000737:chemistry; D007074:Immunoglobulin G / Q000737:chemistry; D049329:Nanostructures / Q000737:chemistry*; D010084:Oxidation-Reduction; D010504:Periodic Acid / Q000737:chemistry; D013388:Succinimides / Q000737:chemistry; D013438:Sulfhydryl Compounds / Q000737:chemistry
chemical_list: D000588:Amines; D000906:Antibodies; D002234:Carbodiimides; D002241:Carbohydrates; D002264:Carboxylic Acids; D004337:Drug Carriers; D006834:Hydrazines; D007074:Immunoglobulin G; D013388:Succinimides; D013438:Sulfhydryl Compounds; D010504:Periodic Acid; C018151:N-succinimidyl 3-(2-pyridyldithio)propionate; C009288:metaperiodate