title: Axicon lens-based cone shell configuration for depth-sensitive fluorescence measurements in turbid media.
abstract: We have developed a novel noncontact setup to implement a cone shell illumination and detection configuration using axicon lenses for depth-sensitive fluorescence measurements. The setup was demonstrated experimentally to be capable of detecting fluorescence from a two-layered turbid agar phantom with a larger sensitivity to the deep layer and a larger range of sensitivity to either layer than a conventional cone configuration implemented by a microscope objective lens. Furthermore, the axicon lens-based setup eliminates the need of moving the objective lens up or down to achieve depth-sensitive measurements, which effectively improves the consistency of optical coupling and thus would be preferred in a clinical setting.
authors.affiliation: School of Chemical and Biomedical Engineering, Nanyang Technological University, Singapore.
authors.forename: Yi Hong
authors.initials: YH
authors.lastname: Ong
authors.forename: Quan
authors.initials: Q
authors.lastname: Liu
mesh_terms: D007909:Lenses; D008029:Lighting; D008853:Microscopy / Q000295:instrumentation*; D013050:Spectrometry, Fluorescence / Q000295:instrumentation*