title: C5a receptor-targeting ligand-mediated delivery of dengue virus antigen to M cells evokes antigen-specific systemic and mucosal immune responses in oral immunization.
abstract: Oral mucosal immunization is a feasible and economic vaccination strategy. In order to achieve a successful oral mucosal vaccination, antigen delivery to gut immune inductive site and avoidance of oral tolerance induction should be secured. One promising approach is exploring the specific molecules expressed on the apical surfaces of M cells that have potential for antigen uptake and immune stimulation. We previously identified complement 5a receptor (C5aR) expression on human M-like cells and mouse M cells and confirmed its non-redundant role as a target receptor for antigen delivery to M cells using a model antigen. Here, we applied the OmpH ligand, which is capable of targeting the ligand-conjugated antigen to M cells to induce specific mucosal and systemic immunities against the EDIII of dengue virus (DENV). Oral immunization with the EDIII-OmpH efficiently targeted the EDIII to M cells and induced EDIII-specific immune responses comparable to those induced by co-administration of EDIII with cholera toxin (CT). Also, the enhanced responses by OmpH were characterized as Th2-skewed responses. Moreover, oral immunization using EDIII-OmpH did not induce systemic tolerance against EDIII. Collectively, we suggest that OmpH-mediated targeting of antigens to M cells could be used for an efficient oral vaccination against DENV infection.
authors.affiliation: Department of Molecular Biology, Chonbuk National University, Jeonju 561-756, Republic of Korea; Institute for Molecular Biology and Genetics, Chonbuk National University, Jeonju 561-756, Republic of Korea.
authors.forename: Sae-Hae
authors.initials: SH
authors.lastname: Kim
authors.forename: In-Young
authors.initials: IY
authors.lastname: Yang
authors.forename: Sun-Hee
authors.initials: SH
authors.lastname: Jang
authors.forename: Ju
authors.initials: J
authors.lastname: Kim
authors.forename: Thang Thua
authors.initials: TT
authors.lastname: Truong
authors.forename: Thuc
authors.initials: T
authors.lastname: Van Pham
authors.forename: Ninh Uyen
authors.initials: NU
authors.lastname: Truong
authors.forename: Kyung-Yeol
authors.initials: KY
authors.lastname: Lee
authors.forename: Yong-Suk
authors.initials: YS
authors.lastname: Jang
mesh_terms: D000284:Administration, Oral; D000818:Animals; D000956:Antigens, Viral / Q000235:genetics / Q000276:immunology*; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000276:immunology*; D053059:Dengue Vaccines / Q000008:administration & dosage* / Q000276:immunology; D003716:Dengue Virus / Q000276:immunology*; D007114:Immunization / Q000379:methods*; D051379:Mice; D011485:Protein Binding; D044087:Receptor, Anaphylatoxin C5a / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology; D018418:Th2 Cells / Q000276:immunology
chemical_list: D000956:Antigens, Viral; D001425:Bacterial Outer Membrane Proteins; D053059:Dengue Vaccines; D044087:Receptor, Anaphylatoxin C5a; D011993:Recombinant Fusion Proteins; C067128:ompH protein, bacteria
keywords: C5a receptor; C5aR; CLSM; CT; DENV; Dengue virus antigen; EDIII; ELISA; ELISPOT; FAE; FITC; GP2; LP; M cell; Mucosal immunity; PP; PPL; Peyer's patch; Peyer's patch lymphocyte; SE; SPL; UEA-1; Ulex europaeus agglutinin 1; Vaccine; WGA; cholera toxin; complement 5a receptor; confocal laser scanning microscopy; dengue virus; envelope domain III; enzyme-linked immunosorbent assay; enzyme-linked immunosorbent spot; fluorescein isothiocyanate; follicle-associated epithelium; glycoprotein 2; lamina propria; splenic lymphocyte; standard error; wheat germ agglutinin