title: Microcontact imprinted surface plasmon resonance sensor for myoglobin detection.
abstract: In this study, we prepared surface plasmon resonance (SPR) sensor using the molecular imprinting technique for myoglobin detection in human serum. For this purpose, we synthesized myoglobin imprinted poly(hydroxyethyl methacrylate-N-methacryloyl-l-tryptophan methyl ester) [poly(HEMA-MATrp)] nanofilm on the surface of SPR sensor. We also synthesized non-imprinted poly(HEMA-MATrp) nanofilm without myoglobin for the control experiments. The SPR sensor was characterized with contact angle measurements, atomic force microscopy, X-ray photoelectron spectroscopy, and ellipsometry. We investigated the effectiveness of the sensor using the SPR system. We evaluated the ability of SPR sensor to sense myoglobin with myoglobin solutions (pH7.4, phosphate buffer) in different concentration range and in the serum taken from a patient with acute myocardial infarction. We found that the Langmuir adsorption model was the most suitable for the sensor system. The detection limit was 87.6 ng/mL. In order to show the selectivity of the SPR sensor, we investigated the competitive detection of myoglobin, lysozyme, cytochrome c and bovine serum albumin. The results showed that the SPR sensor has high selectivity and sensitivity for myoglobin.
authors.affiliation: Uludag University, Department of Chemistry, Bursa, Turkey.
authors.forename: Bilgen
authors.initials: B
authors.lastname: Osman
authors.forename: Lokman
authors.initials: L
authors.lastname: Uzun
authors.forename: Necati
authors.initials: N
authors.lastname: Beşirli
authors.forename: Adil
authors.initials: A
authors.lastname: Denizli
mesh_terms: D000818:Animals; D002417:Cattle; D003199:Computer Systems; D006801:Humans; D007700:Kinetics; D054802:Molecular Imprinting / Q000295:instrumentation* / Q000379:methods*; D009211:Myoglobin / Q000032:analysis*; D059027:Recycling; D012710:Serum Albumin, Bovine / Q000032:analysis; D020349:Surface Plasmon Resonance / Q000295:instrumentation*; D013499:Surface Properties; D013696:Temperature
chemical_list: D009211:Myoglobin; D012710:Serum Albumin, Bovine
keywords: Microcontact imprinting; Molecularly imprinted polymer; Myoglobin; Nanofilm; SPR sensor