title: Expression of tumor necrosis factor-like weak inducer of apoptosis in human middle ear cholesteatoma.
abstract: Tumor necrosis factor (TNF)-like weak inducer of apoptosis (TWEAK) was recently identified as a member of the TNF superfamily of structurally related cytokines. It regulates TNF-α and numerous cellular responses. We investigated whether TWEAK is upregulated in human middle ear cholesteatoma compared to the skin of the normal external auditory canal (EAC). The expression of TWEAK was analyzed using reverse transcription-polymerase chain reaction, Western blotting and immunohistochemical staining. TWEAK expression was correlated with that of TNF-α as determined by Western blotting. The expression of TWEAK and TNF-α protein was stronger in cholesteatoma tissue than in EAC skin. TWEAK was expressed to a greater degree in the suprabasal layer in the cholesteatoma than in EAC skin. The expression of TWEAK was correlated more closely with single-stranded DNA than Ki-67 immunohistochemically. These findings imply that TWEAK plays an important role in modulating TNF-α expression and apoptosis in cholesteatoma.
authors.affiliation: Department of Otolaryngology, Head and Neck Surgery, Soonchunhyang University College of Medicine, Bucheon, Republic of Korea.
authors.forename: M
authors.initials: M
authors.lastname: Park
authors.forename: B
authors.initials: B
authors.lastname: Lee
authors.forename: J
authors.initials: J
authors.lastname: Lee
mesh_terms: D000328:Adult; D015153:Blotting, Western; D018424:Cholesteatoma, Middle Ear / Q000378:metabolism*; D004424:Ear Canal / Q000378:metabolism*; D004432:Ear, Middle / Q000378:metabolism* / Q000473:pathology; D005260:Female; D005786:Gene Expression Regulation / Q000502:physiology*; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D012333:RNA, Messenger / Q000378:metabolism; D018124:Receptors, Tumor Necrosis Factor / Q000235:genetics* / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D000074050:TWEAK Receptor; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D012333:RNA, Messenger; D018124:Receptors, Tumor Necrosis Factor; D000074050:TWEAK Receptor; D014409:Tumor Necrosis Factor-alpha