title: SbcCD-mediated processing of covalent gyrase-DNA complex in Escherichia coli.
abstract: Quinolones trap the covalent gyrase-DNA complex in Escherichia coli, leading to cell death. Processing activities for trapped covalent complex have not been characterized. A mutant strain lacking SbcCD nuclease activity was examined for both accumulation of gyrase-DNA complex and viability after quinolone treatment. Higher complex levels were found in ΔsbcCD cells than in wild-type cells after incubation with nalidixic acid and ciprofloxacin. However, SbcCD activity protected cells against the bactericidal action of nalidixic acid but not ciprofloxacin.
authors.affiliation: Department of Biochemistry & Molecular Biology, New York Medical College, Valhalla, New York, USA.
authors.forename: Sandra
authors.initials: S
authors.lastname: Aedo
authors.forename: Yuk-Ching
authors.initials: YC
authors.lastname: Tse-Dinh
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002939:Ciprofloxacin / Q000494:pharmacology; D027081:DNA Gyrase / Q000378:metabolism*; D004269:DNA, Bacterial / Q000378:metabolism*; D003851:Deoxyribonucleases / Q000378:metabolism*; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology* / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000378:metabolism*; D005092:Exonucleases / Q000378:metabolism*; D009268:Nalidixic Acid / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D029968:Escherichia coli Proteins; C489229:SbcC protein, E coli; D009268:Nalidixic Acid; D002939:Ciprofloxacin; D003851:Deoxyribonucleases; D005092:Exonucleases; C081229:sbcD protein, E coli; D027081:DNA Gyrase
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