title: Arabidopsis thaliana Yellow Stripe1-Like4 and Yellow Stripe1-Like6 localize to internal cellular membranes and are involved in metal ion homeostasis.
abstract: Several members of the Yellow Stripe1-Like (YSL) family of transporter proteins are able to transport metal-nicotianamine (NA) complexes. Substantial progress has been made in understanding the roles of the Arabidopsis YSLs that are most closely related to the founding member of the family, ZmYS1 (e.g., AtYSL1, AtYSL2 and AtYSL3), but there is little information concerning members of the other two well-conserved YSL clades. Here, we provide evidence that AtYSL4 and AtYSL6, which are the only genes in Arabidopsis belong to YSL Group II, are localized to vacuole membranes and to internal membranes resembling endoplasmic reticulum. Both single and double mutants for YSL4 and YSL6 were rigorously analyzed, and have surprisingly mild phenotypes, in spite of the strong and wide-ranging expression of YSL6. However, in the presence of toxic levels of Mn and Ni, plants with mutations in YSL4 and YSL6 and plants overexpressing GFP-tagged YSL6 showed growth defects, indicating a role for these transporters in heavy metal stress responses.
authors.affiliation: Biology, University of Massachusetts Amherst, MA, USA.
authors.forename: S S
authors.initials: SS
authors.lastname: Conte
authors.forename: H H
authors.initials: HH
authors.lastname: Chu
authors.forename: D C
authors.initials: DC
authors.lastname: Rodriguez
authors.forename: T
authors.initials: T
authors.lastname: Punshon
authors.forename: K A
authors.initials: KA
authors.lastname: Vasques
authors.forename: D E
authors.initials: DE
authors.lastname: Salt
authors.forename: E L
authors.initials: EL
authors.lastname: Walker
keywords: endomembrane; iron; manganese; metal transporters; nickel; nicotianamine; tonoplast; yellow stripe-like
references.citation: J Biol Chem. 2004 Mar 5;279(10):9091-6
references.pmid: 14699112
references.citation: Plant Mol Biol. 2009 Aug;70(6):681-92
references.pmid: 19468840
references.citation: Plant Cell Physiol. 2011 Jul;52(7):1142-52
references.pmid: 21613277
references.citation: FEBS Lett. 2007 Sep 4;581(22):4298-302
references.pmid: 17707820
references.citation: Nat Biotechnol. 2003 Oct;21(10):1215-21
references.pmid: 12949535
references.citation: J Biol Chem. 2010 Nov 26;285(48):37263-70
references.pmid: 20876528
references.citation: Plant Cell Physiol. 2004 Dec;45(12):1749-58
references.pmid: 15653794
references.citation: Plant J. 2007 Jan;49(1):1-15
references.pmid: 17144893
references.citation: Trends Plant Sci. 2002 May;7(5):193-5
references.pmid: 11992820
references.citation: Plant J. 2007 Jul;51(2):198-210
references.pmid: 17559518
references.citation: Plant J. 2004 Aug;39(3):403-14
references.pmid: 15255869
references.citation: Plant Physiol. 2004 May;135(1):112-20
references.pmid: 15107503
references.citation: Mol Cell Proteomics. 2007 Mar;6(3):394-412
references.pmid: 17151019
references.citation: Microbiology (Reading). 2001 Nov;147(Pt 11):2881-3
references.pmid: 11700339
references.citation: Proc Natl Acad Sci U S A. 1996 May 28;93(11):5624-8
references.pmid: 8643627
references.citation: Plant Physiol. 2010 Sep;154(1):197-210
references.pmid: 20625001
references.citation: Plant Physiol. 2006 Aug;141(4):1446-58
references.pmid: 16815956
references.citation: Plant J. 2004 Aug;39(3):415-24
references.pmid: 15255870
references.citation: Plant J. 1998 Dec;16(6):735-43
references.pmid: 10069079
references.citation: FEBS Lett. 2005 Aug 1;579(19):4165-74
references.pmid: 16038907
references.citation: Plant J. 2006 May;46(4):563-72
references.pmid: 16640594
references.citation: Plant J. 2010 May;62(3):379-90
references.pmid: 20128878
references.citation: Ann Bot. 2009 Jan;103(1):1-11
references.pmid: 18977764
references.citation: Plant Physiol. 2010 Apr;152(4):1986-99
references.pmid: 20181755
references.citation: Ann Bot. 2011 Oct;108(5):821-33
references.pmid: 21831857
references.citation: J Biol Chem. 2009 Feb 6;284(6):3470-9
references.pmid: 19049971
references.citation: Science. 2003 Aug 1;301(5633):653-7
references.pmid: 12893945
references.citation: Plant J. 2006 Jun;46(5):861-79
references.pmid: 16709200
references.citation: Plant Cell. 2000 Dec;12(12):2395-2408
references.pmid: 11148286
references.citation: EMBO J. 2005 Dec 7;24(23):4041-51
references.pmid: 16270029
references.citation: Plant Physiol. 2011 Dec;157(4):1832-40
references.pmid: 21969384
references.citation: Science. 2006 Nov 24;314(5803):1295-8
references.pmid: 17082420
references.citation: Plant Mol Biol. 1999 May;40(1):37-44
references.pmid: 10394943
references.citation: Plant Cell. 2012 Feb;24(2):724-37
references.pmid: 22374397
references.citation: Proc Natl Acad Sci U S A. 2008 Aug 19;105(33):12081-6
references.pmid: 18697928
references.citation: Plant Mol Biol. 1998 Mar;36(4):521-8
references.pmid: 9484447
references.citation: Plant Methods. 2009 Nov 24;5:16
references.pmid: 19930690
references.citation: Plant J. 2005 Dec;44(5):769-82
references.pmid: 16297069
references.citation: J Cell Biol. 2001 Dec 10;155(6):991-1002
references.pmid: 11739409
references.citation: Plant Cell. 2012 Sep;24(9):3767-82
references.pmid: 23012434
references.citation: Plant Cell. 2012 Jun;24(6):2380-400
references.pmid: 22706286
references.citation: Plant Cell Physiol. 2002 Sep;43(9):965-73
references.pmid: 12354913
references.citation: Plant Physiol. 2009 Nov;151(3):1329-38
references.pmid: 19726572
references.citation: Plant Cell Physiol. 2011 May;52(5):765-74
references.pmid: 21266459
references.citation: Plant Cell. 2013 Mar;25(3):1040-55
references.pmid: 23512854
references.citation: Nature. 2001 Jan 18;409(6818):346-9
references.pmid: 11201743
references.citation: Planta. 2001 Oct;213(6):967-76
references.pmid: 11722133
references.citation: Plant Physiol. 2009 Jun;150(2):786-800
references.pmid: 19376836