title: Bioethanol production from Ipomoea carnea biomass using a potential hybrid yeast strain.
abstract: The paper deals with the exploitation of Ipomoea carnea as a feedstock for the production of bioethanol. Dilute acid pretreatment under optimum conditions (3%H2SO4, 120 °C for 45 min) produced 17.68 g L(-1) sugars along with 1.02 g L(-1) phenolics and 1.13 g L(-1) furans. A combination of overliming and activated charcoal adsorption facilitated the removal of 91.9% furans and 94.7% phenolics from acid hydrolysate. The pretreated biomass was further treated with a mixture of sodium sulphite and sodium chlorite and, a maximum lignin removal of 81.6% was achieved. The enzymatic saccharification of delignified biomass resulted in 79.4% saccharification with a corresponding sugar yield of 753.21 mg g(-1). Equal volume of enzymatic hydrolysate and acid hydrolysate were mixed and used for fermentation with a hybrid yeast strain RPRT90. Fermentation of mixed detoxified hydrolysate at 30 °C for 28 h produced ethanol with a yield of 0.461 g g(-1). A comparable ethanol yield (0.414 g g(-1)) was achieved using a mixture of enzymatic hydrolysate and undetoxified acid hydrolysate. Thus, I. carnea biomass has been demonstrated to be a potential feedstock for bioethanol production, and the use of hybrid yeast may pave the way to produce bioethanol from this biomass.
authors.affiliation: Department of Biotechnology and Medical Engineering, National Institute of Technology, Rourkela, Odisha, India, rajni.nitrkl@gmail.com.
authors.forename: Rajni
authors.initials: R
authors.lastname: Kumari
authors.forename: Krishna
authors.initials: K
authors.lastname: Pramanik
mesh_terms: D000143:Acids; D056804:Biofuels; D018533:Biomass; D002678:Chimera; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation; D005947:Glucose / Q000378:metabolism; D006868:Hydrolysis; D027741:Ipomoea / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism; D014994:Xylose / Q000378:metabolism
chemical_list: D000143:Acids; D056804:Biofuels; D000431:Ethanol; D014994:Xylose; D005947:Glucose