title: High-throughput micro-solid phase extraction on 96-well plate using dodecyl methacrylate-ethylen glycol dimethacrylate monolithic copolymer.
abstract: A novel high-throughput device based on 96-micro-solid phase extraction (96-μ-SPE) system was constructed for multiresidue determination of nine pesticides in aquatic samples. The extraction procedure was performed on a commercially available 96-well plate system. The extraction module consisted of 96 pieces of 1cm×3cm of cylindrically shaped stainless steel meshes. The prepared meshes were fixed in a home-made polytetrafluoroethylene-based constructed ninety-six holes block for possible simultaneous immersion of meshes into the center of individual wells. Dodecyl methacrylate and ethylene glycol dimethacrylate was copolymerized as a monolithic polymer and placed in the cylindrically shaped stainless steel meshes as extracting medium. A volume of 1mL of the aquatic sample was transferred into the 96-well plate and the 96-μ-SPE device was applied for the extraction of the selected pesticides. Subsequently, the extracted analytes were analyzed by gas chromatography-mass spectrometry. Influential parameters such as polymer synthesis conditions, sorbent-to-sorbent reproducibility, ionic strength and extraction time were optimized. Intra and inter-sorbent reproducibility on 96-μ-SPE device were evaluated and results revealed that extraction yields are rather similar. Limits of detection were below 4μg L(-1) and the coefficient of determination was satisfactory (r(2)>0.99) for all the studied analytes. The developed method was successfully applied to the extraction and determination of the selected pesticides in surface water samples.
authors.affiliation: Environmental and Bio-Analytical Laboratories, Department of Chemistry, Sharif University of Technology, P.O. Box 11365-9516, Tehran, Iran. bagheri@sharif.edu
authors.forename: Habib
authors.initials: H
authors.lastname: Bagheri
authors.forename: Ali
authors.initials: A
authors.lastname: Es'haghi
authors.forename: Ali
authors.initials: A
authors.lastname: Es-haghi
authors.forename: Elham
authors.initials: E
authors.lastname: Mohammadkhani
mesh_terms: D004784:Environmental Monitoring / Q000379:methods*; D008689:Methacrylates / Q000032:analysis*; D008855:Microscopy, Electron, Scanning; D010573:Pesticide Residues / Q000032:analysis*; D045483:Rivers / Q000737:chemistry*; D052616:Solid Phase Extraction; D014874:Water Pollutants, Chemical / Q000032:analysis*
chemical_list: D008689:Methacrylates; D010573:Pesticide Residues; D014874:Water Pollutants, Chemical; C115846:dodecyl methacrylate; C004919:ethylene dimethacrylate
keywords: 96-Well plate; High-throughput; Micro-solid phase extraction; Monolithic polymer; Pesticide multiresidue