title: Classical activation of microglia in CD200-deficient mice is a consequence of blood brain barrier permeability and infiltration of peripheral cells.
abstract: The interaction between CD200, expressed on several cell types, and its receptor CD200R, expressed on cells of the myeloid lineage, has been shown to be an important factor in modulating inflammation in macrophage function in several conditions including colitis and arthritis. More recently its modulatory effect on microglial activation has been identified and CD200-deficiency has been associated with increased microglial activation accompanied by increased production of inflammatory cytokines. The response of glia prepared from CD200-deficient mice to stimuli like lipopolysaccharide (LPS) is markedly greater than the response of cells prepared from wildtype mice and, consistent with this, is the recent observation that expression of Toll-like receptor (TLR)4 and signalling through NFκB are increased in microglia prepared from CD200-deficient mice. Here we show that glia from CD200-deficient mice are also more responsive to interferon-γ (IFNγ) which triggers classical activation of microglia. We investigated the effects of CD200-deficiency in vivo and report that there is an increase in expression of several markers of microglial activation including tumor necrosis factor (TNF)-α, which is a hallmark of classically-activated microglia. These changes are accompanied by increased IFNγ, and the evidence suggests that this is produced by infiltrating cells including T cells and macrophages. We propose that these cells enter the brain as a consequence of increased blood brain barrier (BBB) permeability in CD200-deficient mice and that infiltration is assisted by increased expression of the chemokines, monocyte chemotactic protein-1 (MCP-1), IFNγ-induced protein-10 (IP-10) and RANTES. This may have implications in neurodegenerative diseases where BBB permeability is compromised.
authors.affiliation: Trinity College Institute for Neuroscience, Trinity College, Dublin 2, Ireland.
authors.forename: Stephanie
authors.initials: S
authors.lastname: Denieffe
authors.forename: Ronan J
authors.initials: RJ
authors.lastname: Kelly
authors.forename: Claire
authors.initials: C
authors.lastname: McDonald
authors.forename: Anthony
authors.initials: A
authors.lastname: Lyons
authors.forename: Marina A
authors.initials: MA
authors.lastname: Lynch
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000235:genetics*; D001812:Blood-Brain Barrier / Q000276:immunology* / Q000378:metabolism; D002540:Cerebral Cortex / Q000378:metabolism; D006624:Hippocampus / Q000378:metabolism; D051379:Mice; D018345:Mice, Knockout; D017628:Microglia / Q000276:immunology* / Q000378:metabolism; D010539:Permeability
chemical_list: D015703:Antigens, CD; C042739:antigens, CD200
keywords: Blood brain barrier permeability; CD200; Claudin; Inflammation; Lipopolysaccharide; Microglial activation