title: Continuous lipid extraction of microalgae using high-pressure carbon dioxide.
abstract: Sequestering carbon, purifying water, and creating biofuel materials using microalgae are of global interest in the R&D field. However, extracting algal oil consumes a high amount of energy, which is an obstacle for the biofuel market. Nontoxic and recyclable high-pressure CO2 extraction processes are being developed by numerous researchers; however, most of these processes use batch operations mixed with a large amount of co-solvent and require improvement. We fabricated a continuous high-pressure CO2 extraction system, evaluating the optimal parameters for the extraction process. The various parameter tests included temperature, pressure, pretreatment methods, ratio, and the species of co-solvent. We integrated the optimal parameters from previous tests, using a 5-d continuous operation. Compared with traditional solvent extraction, a 90.56% extraction yield ratio was achieved using this continuous extraction method. This shows the stable, high extraction yields of this continuous high-pressure CO2 extraction system.
authors.affiliation: Green Energy and Environmental Laboratory, Industrial Technology Research Institute, Taiwan, ROC. Electronic address: ktchen@itri.org.tw.
authors.forename: Kuo-Ti
authors.initials: KT
authors.lastname: Chen
authors.affiliation: Green Energy and Environmental Laboratory, Industrial Technology Research Institute, Taiwan, ROC.
authors.forename: Chen-Hsi
authors.initials: CH
authors.lastname: Cheng
authors.affiliation: Green Energy and Environmental Laboratory, Industrial Technology Research Institute, Taiwan, ROC.
authors.forename: Yi-Huei
authors.initials: YH
authors.lastname: Wu
authors.affiliation: Green Energy and Environmental Laboratory, Industrial Technology Research Institute, Taiwan, ROC.
authors.forename: Wen-Chang
authors.initials: WC
authors.lastname: Lu
authors.affiliation: Green Energy and Environmental Laboratory, Industrial Technology Research Institute, Taiwan, ROC.
authors.forename: Yun-Huin
authors.initials: YH
authors.lastname: Lin
authors.affiliation: Green Energy and Environmental Laboratory, Industrial Technology Research Institute, Taiwan, ROC.
authors.forename: Hom-Ti
authors.initials: HT
authors.lastname: Lee
mesh_terms: D056804:Biofuels; D002244:Carbon / Q000737:chemistry*; D002245:Carbon Dioxide / Q000737:chemistry*; D002849:Chromatography, Gas; D025924:Chromatography, Supercritical Fluid / Q000379:methods; D004867:Equipment Design; D008055:Lipids / Q000302:isolation & purification*; D058086:Microalgae / Q000378:metabolism*; D011312:Pressure; D012997:Solvents / Q000737:chemistry; D013696:Temperature; D013997:Time Factors
chemical_list: D056804:Biofuels; D008055:Lipids; D012997:Solvents; D002245:Carbon Dioxide; D002244:Carbon
keywords: Biofuel; FAME; Microalgae