title: Electrospun adherent-antiadherent bilayered membranes based on cross-linked hyaluronic acid for advanced tissue engineering applications.
abstract: A procedure to obtain electrospun mats of hyaluronic acid (HA) stable in aqueous media in one single step has been developed. It consists in combining an HA solution with a divinyl sulfone one as cross-linker in a three-way valve to immediately electroblow their mixture. Membranes obtained with this method, after sterilization and conditioning, are ready to use in cell culture without need of any additional post-treatment. HA nanofibers are deposited onto previously electrospun poly(l-lactic acid) (PLLA) mats in order to obtain stably joined bilayered membranes with an adherent face and the opposite face non-adherent, despite their different hydrophilicity and mechanical properties. These bilayered HA/PLLA membranes may be of use, for example, in applications seeking to transplant cells on a tissue surface and keep them protected from the environment: the PLLA nanofiber face is cell friendly and promotes cell attachment and spreading and can thus be used as a cell supply vehicle, while the HA face hinders cell adhesion and thus may prevent post-surgical adherences, a major issue in many surgeries.
authors.affiliation: Center for Biomaterials and Tissue Engineering, Universitat Politècnica de Valencia, C. de Vera s/n, 46022, Valencia, Spain.
authors.forename: M
authors.initials: M
authors.lastname: Arnal-Pastor
authors.forename: C
authors.initials: C
authors.lastname: Martínez Ramos
authors.forename: M
authors.initials: M
authors.lastname: Pérez Garnés
authors.forename: M
authors.initials: M
authors.lastname: Monleón Pradas
authors.forename: A
authors.initials: A
authors.lastname: Vallés Lluch
mesh_terms: D000268:Adhesiveness; D000818:Animals; D002460:Cell Line; D003432:Cross-Linking Reagents / Q000494:pharmacology*; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000648:ultrastructure; D006820:Hyaluronic Acid / Q000737:chemistry / Q000494:pharmacology*; D019344:Lactic Acid / Q000737:chemistry / Q000494:pharmacology; D008567:Membranes, Artificial; D051379:Mice; D008856:Microscopy, Fluorescence; D008970:Molecular Weight; D011091:Polyesters; D011108:Polymers / Q000737:chemistry / Q000494:pharmacology; D013818:Thermogravimetry; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D003432:Cross-Linking Reagents; D008567:Membranes, Artificial; D011091:Polyesters; D011108:Polymers; D019344:Lactic Acid; C033616:poly(lactide); D006820:Hyaluronic Acid
keywords: Cross-linking; Electrospinning; Hyaluronic acid; Membrane; Poly(l-lactic acid)