title: Influences of NR2B-containing NMDA receptors knockdown on neural activity in hippocampal newborn neurons.
abstract: Adult-born neurons undergo a transient period of plasticity during their integration into the neural circuit. This transient plasticity may involve NMDA receptors containing NR2B, the major subunit expressed at early developmental stages. The main objective of the present study was to investigate the effects of NR2B gene knockdown on the functional integration of the adult-born granule cells generated from the subgranule zone (SGZ) in the hippocampus. The small interfering RNA (siRNA) was used to knock down the NR2B gene in the adult-born hippocampal neurons. In the functional integration test, the mice were exposed to a novel environment (open field arena), and the expression of c-fos was immunohistochemically detected in the hippocampus. After exposure to the novel environment, siRNA-NR2B mice were significantly different from control mice in either the number of squares or the number of rears they crossed, showing decreased horizontal and vertical activity (P<0.05). Moreover, the c-fos expression was increased in both control and siRNA-NR2B mice after open field test. But, it was significantly lower in siRNA-NR2B neurons than in control neurons. It was concluded that the neural activity of newborn neurons is regulated by their own NR2B-containing NMDA glutamate receptors during a short, critical period after neuronal birth.
authors.affiliation: Department of Neurology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China. silled@126.com.
authors.forename: Zhi-Jun
authors.initials: ZJ
authors.lastname: Li
authors.affiliation: Department of Neurology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China.|Department of Function Examination, The Second Affiliated Hospital, Nanhua University, Hengyang, 421001, China.
authors.forename: Hui-Wen
authors.initials: HW
authors.lastname: Zhang
authors.affiliation: Department of Neurology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China.
authors.forename: Na
authors.initials: N
authors.lastname: Tang
mesh_terms: D000818:Animals; D005260:Female; D055785:Gene Knockdown Techniques; D006624:Hippocampus / Q000502:physiology*; D008297:Male; D051379:Mice; D009474:Neurons / Q000502:physiology*; D016194:Receptors, N-Methyl-D-Aspartate / Q000235:genetics*
chemical_list: C121001:NR2B NMDA receptor; D016194:Receptors, N-Methyl-D-Aspartate
references.citation: Gene Ther. 2005 Jan;12(1):59-66
references.pmid: 15470478
references.citation: Nat Neurosci. 2008 Oct;11(10):1153-61
references.pmid: 18758458
references.citation: Nat Neurosci. 2003 May;6(5):507-18
references.pmid: 12704391
references.citation: J Neurosci. 2005 Nov 2;25(44):10074-86
references.pmid: 16267214
references.citation: Nature. 2006 Aug 24;442(7105):929-33
references.pmid: 16906136
references.citation: Hippocampus. 1993 Apr;3(2):165-82
references.pmid: 8353604
references.citation: Science. 1988 Jun 3;240(4857):1328-31
references.pmid: 3131879
references.citation: Neuron. 2007 May 24;54(4):559-66
references.pmid: 17521569
references.citation: Behav Neurosci. 2005 Feb;119(1):145-53
references.pmid: 15727520
references.citation: J Neurosci. 2006 Jan 4;26(1):3-11
references.pmid: 16399667
references.citation: Cell Stem Cell. 2012 Jan 6;10(1):76-87
references.pmid: 22226357
references.citation: Annu Rev Neurosci. 2010;33:131-49
references.pmid: 20572770
references.citation: Science. 1987 Jul 10;237(4811):192-7
references.pmid: 3037702
references.citation: Eur J Neurosci. 2002 Jul;16(1):129-36
references.pmid: 12153537
references.citation: J Neurosci. 2005 Nov 16;25(46):10729-39
references.pmid: 16291946
references.citation: J Neurosci. 2012 Sep 5;32(36):12603-11
references.pmid: 22956849
references.citation: Neuron. 2010 Jan 14;65(1):32-9
references.pmid: 20152111
references.citation: Science. 1994 May 20;264(5162):1145-8
references.pmid: 8178174
references.citation: J Neurosci. 2009 Sep 23;29(38):11852-8
references.pmid: 19776271
references.citation: J Neurosci. 2006 Oct 11;26(41):10508-13
references.pmid: 17035535
references.citation: Philos Trans R Soc Lond B Biol Sci. 1971 Jul 1;262(841):23-81
references.pmid: 4399412
references.citation: Science. 2007 Jul 6;317(5834):94-9
references.pmid: 17556551
references.citation: Proc Natl Acad Sci U S A. 2006 Nov 14;103(46):17501-6
references.pmid: 17088541
references.citation: Annu Rev Neurosci. 1991;14:453-501
references.pmid: 2031577
references.citation: J Neurosci. 2009 Oct 28;29(43):13532-42
references.pmid: 19864566
references.citation: Nat Neurosci. 2007 Mar;10(3):355-62
references.pmid: 17277773