title: Reversed-phase High-performance Liquid Chromatography Method for Analysis of Curcuminoids and Curcuminoid-loaded Liposome Formulation.
abstract: A simple, sensitive, precise and specific method for the determination of curcuminoids and curcuminoid-loaded liposome formulation was developed using reverse-phase high-performance liquid chromatography method. The analysis was performed isocratically on Zorbax Eclipse XDB-C18 column (150×4 mm, 5 μm), analytical column using UV detector and mobile phase consisting of 0.1% orthophosphoric acid and acetonitrile. The proposed method for curcuminoids was validated for linearity in the range from 50 to 300 ΅g/ml with correlation coefficient above 0.997. Intraday and interday precision studies showed the relative standard deviation less than 2%. The limit of detection and limit of quantitation values were 2.5 and 8.25 ΅g/ml, respectively. Forced degradation study for curcuminoids and liposomal curcuminoids sample was carried out and observed that proposed method was also suitable for finding degradation products in the sample. Proposed method was successfully applied to estimate curcuminoids content without any interference of other excipients from liposomal formulation. Therefore, the method developed is well suited for curcuminoids and its liposome estimation.
authors.affiliation: Department of Chemical Engineering, Advanced Drying Laboratory, Institute of Chemical Technology (Formerly UDCT), N. P. Road, Matunga (E), Mumbai-400 019, India.
authors.forename: R D
authors.initials: RD
authors.lastname: Jangle
authors.forename: B N
authors.initials: BN
authors.lastname: Thorat
keywords: Curcuminoids; degradation study; liposome; reversed phase-high performance liquid chromatography
references.citation: Indian J Pharm Sci. 2010 Mar;72(2):149-54
references.pmid: 20838516
references.citation: Cancer Lett. 2008 Aug 18;267(1):133-64
references.pmid: 18462866
references.citation: J Nanobiotechnology. 2007 Apr 17;5:3
references.pmid: 17439648
references.citation: Eur J Pharm Sci. 2009 Jun 28;37(3-4):395-404
references.pmid: 19491031
references.citation: Indian J Pharm Sci. 2008 Jul-Aug;70(4):445-9
references.pmid: 20046768
references.citation: AAPS J. 2009 Sep;11(3):495-510
references.pmid: 19590964
references.citation: Int J Cancer. 2009 Jul 1;125(1):1-8
references.pmid: 19326431
references.citation: Int J Pharm. 2009 Jan 21;366(1-2):133-9
references.pmid: 18840516
references.citation: J Chromatogr B Analyt Technol Biomed Life Sci. 2003 Nov 5;796(2):339-46
references.pmid: 14581073
references.citation: Eur J Pharm Sci. 2009 Jun 28;37(3-4):223-30
references.pmid: 19491009
references.citation: J Agric Food Chem. 2002 Jun 19;50(13):3668-72
references.pmid: 12059141
references.citation: J Chromatogr A. 2002 Jul 12;962(1-2):117-25
references.pmid: 12198956
references.citation: Biochem Pharmacol. 2008 Feb 15;75(4):787-809
references.pmid: 17900536
references.citation: J Pharm Biomed Anal. 2005 Sep 1;39(1-2):132-8
references.pmid: 15941643
references.citation: Drug Metab Dispos. 1999 Apr;27(4):486-94
references.pmid: 10101144
references.citation: Biochem Pharmacol. 2008 Dec 1;76(11):1590-611
references.pmid: 18775680
references.citation: Int J Pharm. 2009 Apr 17;371(1-2):148-55
references.pmid: 19124065
references.citation: Cancer. 2005 Sep 15;104(6):1322-31
references.pmid: 16092118
references.citation: Antioxid Redox Signal. 2008 Mar;10(3):511-45
references.pmid: 18370854
references.citation: Planta Med. 2000 May;66(4):396-8
references.pmid: 10865470
references.citation: Clin Cancer Res. 2008 Jul 15;14(14):4491-9
references.pmid: 18628464
references.citation: Mol Pharm. 2007 Nov-Dec;4(6):807-18
references.pmid: 17999464
references.citation: Int J Biochem Cell Biol. 2009 Jan;41(1):40-59
references.pmid: 18662800
references.citation: J Pharm Pharmacol. 2009 Mar;61(3):311-21
references.pmid: 19222903
references.citation: Biomed Chromatogr. 2009 Nov;23(11):1201-7
references.pmid: 19488971
references.citation: J Pharm Biomed Anal. 2005 Jun 1;38(1):133-8
references.pmid: 15907631