title: A peroxisome biogenesis deficiency prevents the binding of alpha-synuclein to lipid droplets in lipid-loaded yeast.
abstract: Using a yeast model of Parkinson's disease, we found that alpha-synuclein (αS) binds to lipid droplets in lipid-loaded, wild-type yeast cells but not to lipid droplets in lipid-loaded, peroxisome-deficient cells (pex3Δ). Our analysis revealed that pex3Δ cells have both fewer lipid droplets and smaller lipid droplets than wild-type cells, and that the acyl chains of the phospholipids on the surface of the lipid droplets from pex3Δ cells are on average shorter (C16) than those (C18) on the surface of lipid droplets from wild-type cells. We propose that the shift to shorter (C18→C16) acyl chains contributes to the reduced binding of αS to lipid droplets in pex3Δ cells.
authors.affiliation: Department of Biochemistry and Molecular Biology, Louisiana State University Health Sciences Center, Shreveport, LA 71130, USA.
authors.forename: Shaoxiao
authors.initials: S
authors.lastname: Wang
authors.forename: Patrick J
authors.initials: PJ
authors.lastname: Horn
authors.forename: Liang-Chun
authors.initials: LC
authors.lastname: Liou
authors.forename: Martin I
authors.initials: MI
authors.lastname: Muggeridge
authors.forename: Zhaojie
authors.initials: Z
authors.lastname: Zhang
authors.forename: Kent D
authors.initials: KD
authors.lastname: Chapman
authors.forename: Stephan N
authors.initials: SN
authors.lastname: Witt
mesh_terms: D005656:Fungal Proteins / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000378:metabolism; D057927:Hydrophobic and Hydrophilic Interactions; D008055:Lipids / Q000737:chemistry; D008565:Membrane Proteins / Q000235:genetics; D010300:Parkinson Disease / Q000378:metabolism; D000074401:Peroxins; D020675:Peroxisomes / Q000378:metabolism*; D010743:Phospholipids / Q000737:chemistry; D010957:Plasmids; D011485:Protein Binding; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics; D013499:Surface Properties; D051844:alpha-Synuclein / Q000378:metabolism*
chemical_list: D005656:Fungal Proteins; D008055:Lipids; D008565:Membrane Proteins; C070895:PEX3 protein, S cerevisiae; D000074401:Peroxins; D010743:Phospholipids; D029701:Saccharomyces cerevisiae Proteins; D051844:alpha-Synuclein; D049452:Green Fluorescent Proteins
keywords: Alpha-synuclein; Lipid droplets; Parkinson’s disease; Peroxisome
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