title: X-ray crystallographic evidence for the presence of the cysteine tryptophylquinone cofactor in L-lysine ε-oxidase from Marinomonas mediterranea.
abstract: We have determined the x-ray crystal structure of L-lysine ε-oxidase from Marinomonas mediterranea in its native and L-lysine-complex forms at 1.94- and 1.99-Å resolution, respectively. In the native enzyme, electron densities clearly indicate the presence of cysteine tryptophylquinone (CTQ) previously identified in quinohemoprotein amine dehydrogenase. In the L-lysine-complex, an electron density corresponding to the bound L-lysine shows that its ε-amino group is attached to the C6 carbonyl group of CTQ, suggesting the formation of a Schiff-base intermediate. Collectively, the present crystal structure provides the first example of an enzyme employing a tryptophylquinone cofactor in an amine oxidase.
authors.affiliation: Biotechnology Research Center and Department of Biotechnology, Toyama Prefectural University, 5180 Kurokawa, Imizu, Toyama 939-0398, Japan.
authors.forename: Seiji
authors.initials: S
authors.lastname: Okazaki
authors.forename: Shogo
authors.initials: S
authors.lastname: Nakano
authors.forename: Daisuke
authors.initials: D
authors.lastname: Matsui
authors.forename: Shusaku
authors.initials: S
authors.lastname: Akaji
authors.forename: Kenji
authors.initials: K
authors.lastname: Inagaki
authors.forename: Yasuhisa
authors.initials: Y
authors.lastname: Asano
mesh_terms: D000594:Amino Acid Oxidoreductases / Q000737:chemistry*; D001426:Bacterial Proteins / Q000737:chemistry*; D020134:Catalytic Domain; D003067:Coenzymes / Q000737:chemistry*; D018360:Crystallography, X-Ray; D004151:Dipeptides / Q000737:chemistry*; D004583:Electrons; D045563:Indolequinones / Q000737:chemistry*; D007700:Kinetics; D053544:Marinomonas / Q000737:chemistry* / Q000201:enzymology; D008958:Models, Molecular; D012545:Schiff Bases / Q000737:chemistry
chemical_list: D001426:Bacterial Proteins; D003067:Coenzymes; D004151:Dipeptides; D045563:Indolequinones; D012545:Schiff Bases; C447619:cysteine tryptophylquinone; D000594:Amino Acid Oxidoreductases; C041123:L-lysine oxidase
keywords: CTQ; crystal structure; iodide SAD; l-lysine; oxidative deamination