title: Infectivity of a genotype 4 hepatitis E virus cDNA clone by intrahepatic inoculation of laboratory rats.
abstract: A cDNA clone of a genotype 4 swine hepatitis E virus (HEV) strain (SAAS-FX17), identified in Shanghai, has been constructed. Capped RNA transcripts were prepared in vitro and shown to be replication-competent in Huh7 cells. Sprague-Dawley (SD) rats administered the RNA transcripts by intrahepatic inoculation developed active infections as evidenced by fecal virus shedding and sero-conversion to anti-HEV. The former was first detected between 23 and 30 days post-inoculation (dpi) and persisted until 45 dpi. Sera of rats inoculated with RNA transcripts became anti-HEV positive between 30 and 40 dpi, and reverted to anti-HEV negative at 52 dpi. Our data indicate for the first time that intrahepatic inoculation of rats with RNA transcripts of an HEV cDNA clone may serve as an alternative animal model for HEV research.
authors.affiliation: Institute of Animal Science and Veterinary Medicine, Shanghai Academy of Agricultural Sciences, Shanghai, China.
authors.forename: Yumin
authors.initials: Y
authors.lastname: Zhu
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Yu
authors.forename: Yuanshu
authors.initials: Y
authors.lastname: Zhang
authors.forename: Yanyan
authors.initials: Y
authors.lastname: Ni
authors.forename: Fusheng
authors.initials: F
authors.lastname: Si
authors.forename: Ruisong
authors.initials: R
authors.lastname: Yu
authors.forename: Shijuan
authors.initials: S
authors.lastname: Dong
authors.forename: Yaowei
authors.initials: Y
authors.lastname: Huang
authors.forename: Zhen
authors.initials: Z
authors.lastname: Li
mesh_terms: D000818:Animals; D000914:Antibodies, Viral; D002681:China; D018076:DNA, Complementary / Q000235:genetics / Q000378:metabolism; D004279:DNA, Viral / Q000235:genetics* / Q000378:metabolism; D004195:Disease Models, Animal; D005260:Female; D005838:Genotype; D016751:Hepatitis E / Q000276:immunology / Q000662:veterinary* / Q000821:virology; D016752:Hepatitis E virus / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity* / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013552:Swine; D013553:Swine Diseases / Q000276:immunology / Q000821:virology*; D017201:Virus Shedding
chemical_list: D000914:Antibodies, Viral; D018076:DNA, Complementary; D004279:DNA, Viral
keywords: Dpi; Genotype 4; HEV; Hepatitis E virus (HEV); IFA; Infectious clone; Rat animal model; days post-inoculation; hepatitis E virus; immunofluorescence assay