title: Influence of the addition of antibiotics on survival of herpesvirus of turkeys.
abstract: To determine the influence of the antibiotics ceftiofur sodium from two different laboratories (A and B) and gentamycin sulfate on a Marek's disease commercial vaccine herpesvirus of turkey (HVT), samples were assayed by titration in chicken embryo fibroblasts (CEF). Viruses were tested in vitro to establish the average number of plaque-forming units before and after different periods of incubation with the addition of the antibiotic. These tests showed no effect of gentamycin or ceftiofur A or B on HVT titers when treatments were for 1 hr or less. However, ceftiofur B decreased the titer at 2 hr. The in vivo effects of the antibiotics were determined by vaccinating 15 one-day-old chickens with HVT plus gentamycin or ceftiofur A or B. Birds were considered viremic at 1 wk postvaccination when one or more plaques were detected in CEF 5 days after inoculation of peripheral blood lymphocytes. Viremia levels were similar between 1 and 16 wk after vaccination with HVT with ceftiofur A or B. The pH values (7.5) were the same in vaccines with and without antibiotics.
authors.affiliation: Comisión de Investigaciones Científicas de la Provincia Buenos Aires, Argentina. cb235@yahoo.com
authors.forename: Celina
authors.initials: C
authors.lastname: Buscaglia
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage*; D002478:Cells, Cultured; D002511:Cephalosporins / Q000008:administration & dosage*; D002642:Chick Embryo; D002645:Chickens; D005839:Gentamicins / Q000008:administration & dosage*; D026002:Herpesvirus 1, Meleagrid / Q000235:genetics / Q000276:immunology*; D022501:Marek Disease Vaccines / Q000008:administration & dosage / Q000276:immunology*; D013047:Specific Pathogen-Free Organisms; D013997:Time Factors
chemical_list: D000900:Anti-Bacterial Agents; D002511:Cephalosporins; D005839:Gentamicins; D022501:Marek Disease Vaccines; C053503:ceftiofur