title: Molecular characterization of Giardia duodenalis isolates from police and farm dogs in China.
abstract: To assess the potential zoonotic transmission of giardiasis from dogs in China, a total of 205 fecal specimens from dogs were screened for Giardia duodenalis using PCR and sequence analysis of the triosephosphate isomerase gene. The prevalence of G. duodenalis in dogs was 13.2% (27/205). The potentially zoonotic assemblage A and the dog-specific assemblage C was identified in 25 (12.2%) and two (1.0%) dogs, respectively. All assemblage A isolates belonged to sub-assemblage AI, genotype AI-1. Likewise, one subtype was found in assemblage C. The high occurrence of potentially zoonotic G. duodenalis subtype AI-1 in dogs that are in close contact with humans is of public health concern.
authors.affiliation: College of Veterinary Medicine, Northeast Agricultural University, 59 Mucai Street, Harbin 150030, China; College of Animal Science and Veterinary Medicine, Jilin University, 5333 Xi'an Road, Changchun 130062, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.forename: Chengwu
authors.initials: C
authors.lastname: Liu
authors.forename: Yuqiang
authors.initials: Y
authors.lastname: Yu
authors.forename: Jianhua
authors.initials: J
authors.lastname: Li
authors.forename: Pengtao
authors.initials: P
authors.lastname: Gong
authors.forename: Mingxin
authors.initials: M
authors.lastname: Song
authors.forename: Lihua
authors.initials: L
authors.lastname: Xiao
authors.forename: Xichen
authors.initials: X
authors.lastname: Zhang
mesh_terms: D000818:Animals; D001483:Base Sequence; D002681:China / Q000453:epidemiology; D016054:DNA, Protozoan / Q000737:chemistry / Q000302:isolation & purification; D004283:Dog Diseases / Q000453:epidemiology / Q000469:parasitology*; D004285:Dogs; D005243:Feces / Q000469:parasitology; D005260:Female; D005819:Genetic Markers; D005838:Genotype; D016829:Giardia lamblia / Q000145:classification / Q000235:genetics*; D005873:Giardiasis / Q000453:epidemiology / Q000469:parasitology / Q000662:veterinary*; D008297:Male; D016133:Polymerase Chain Reaction / Q000662:veterinary; D014305:Triose-Phosphate Isomerase / Q000235:genetics
chemical_list: D016054:DNA, Protozoan; D005819:Genetic Markers; D014305:Triose-Phosphate Isomerase
keywords: Dog; Genotyping; Giardia duodenalis; Subtyping; Triosephosphate isomerase