title: Multilayer nanoparticles for sustained delivery of exenatide to treat type 2 diabetes mellitus.
abstract: A method for the sustained delivery of exenatide was proposed using nanoparticles (NPs) with a core/shell structure. The interactions between lipid bilayers and Pluronics were utilized to form various NPs using a layer-by-layer approach. Transmittance electron microscopy and dynamic light scattering were used to examine the morphology of the NPs. The in vitro release pattern was observed as a function of changes in the structure of the NPs, and the structural integrity of exenatide released was examined by SDS-PAGE analysis. Pharmacokinetics and antidiabetic effects were also observed with the structural change of NPs using in vivo animal models. In vitro-in vivo correlation was discussed in relation to manipulation of the NP structures.
authors.affiliation: College of Pharmacy, Korea University, 2511 Sejongro, Sejong 339-700, Republic of Korea.
authors.forename: Jae Yeon
authors.initials: JY
authors.lastname: Kim
authors.forename: Hwanbum
authors.initials: H
authors.lastname: Lee
authors.forename: Keun Sang
authors.initials: KS
authors.lastname: Oh
authors.forename: Seho
authors.initials: S
authors.lastname: Kweon
authors.forename: Ok-cheol
authors.initials: OC
authors.lastname: Jeon
authors.forename: Youngro
authors.initials: Y
authors.lastname: Byun
authors.forename: Kwangmeyung
authors.initials: K
authors.lastname: Kim
authors.forename: Ick Chan
authors.initials: IC
authors.lastname: Kwon
authors.forename: Sang Yoon
authors.initials: SY
authors.lastname: Kim
authors.forename: Soon Hong
authors.initials: SH
authors.lastname: Yuk
mesh_terms: D000818:Animals; D003924:Diabetes Mellitus, Type 2 / Q000188:drug therapy*; D004591:Electrophoresis, Polyacrylamide Gel; D000077270:Exenatide; D008051:Lipid Bilayers / Q000737:chemistry; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D053758:Nanoparticles / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry / Q000627:therapeutic use*; D014688:Venoms / Q000737:chemistry / Q000627:therapeutic use*
chemical_list: D008051:Lipid Bilayers; D010455:Peptides; D014688:Venoms; D000077270:Exenatide
keywords: Core/shell nanoparticles; Layer-by-layer approach; Lipid bilayers; Pluronics; Sustained delivery of exenatide