title: In vitro myristoylation assay of Arabidopsis proteins.
abstract: Myristoylation is a lipid modification conserved among eukaryotes and involves the addition of a 14-carbon myristoyl moiety to a glycine at the N-terminus of cargo proteins. Since not every protein with an N-terminal glycine is myristoylated, experimental verification is necessary to determine which proteins are indeed myristoylated. Here we describe an in vitro myristoylation assay for the Arabidopsis heterotrimeric G protein alpha subunit, GPA1, as well as the Arabidopsis SALT OVERLY SENSITIVE3. This method can be easily adopted to other proteins of interest.
authors.affiliation: Monsanto Company, St. Louis, MO, USA.
authors.forename: Xuehui
authors.initials: X
authors.lastname: Feng
authors.forename: Wan
authors.initials: W
authors.lastname: Shi
authors.forename: Xuejun
authors.initials: X
authors.lastname: Wang
authors.forename: Mark P
authors.initials: MP
authors.lastname: Running
mesh_terms: D029681:Arabidopsis Proteins / Q000378:metabolism*; D005229:Fatty Acids, Monounsaturated / Q000378:metabolism*; D044385:GTP-Binding Protein alpha Subunits / Q000378:metabolism*; D005998:Glycine / Q000378:metabolism; D050356:Lipid Metabolism; D011499:Protein Processing, Post-Translational
chemical_list: D029681:Arabidopsis Proteins; D005229:Fatty Acids, Monounsaturated; C429765:GPA1 protein, Arabidopsis; D044385:GTP-Binding Protein alpha Subunits; C054211:9-tetradecenoic acid; D005998:Glycine