title: Evaluation of genotoxicity and antigenotoxicity of artepillin C in V79 cells by the comet and micronucleus assays.
abstract: Artepillin C (3,5-diprenyl-p-coumaric acid) is one of the major phenolic compounds found in Brazilian green propolis, as well as in its botanical source, Baccharis dracunculifolia DC (Asteraceae). The present study evaluated the possible genotoxic and protective activities of artepillin C, in vitro, using methyl methanesulfonate (MMS) as a positive control, by comet and micronucleus assays. The cultures of Chinese hamster lung fibroblasts (V79 cells) were treated with different concentrations of artepillin C (2.5, 5.0, 10.0, and 20 μM). In antigenotoxicity assessment, the 3 concentrations of artepillin C (2.5, 5.0, and 10.0 μM) were associated with MMS (200 μM-comet assay and 400 μM-micronucleus assay). A statistically significant increase in the DNA damage and micronucleus frequencies was observed in the culture treated with the highest concentration of the artepillin C in comparison to the control group. All concentrations of artepillin C showed protective activity in relation to MMS-induced genotoxicity, which may be due to its antioxidant properties.
authors.affiliation: a Universidade de Franca , Franca , São Paulo , Brazil.
authors.forename: Pollyanna Francielli
authors.initials: PF
authors.lastname: de Oliveira
authors.forename: Ildercílio Mota de Souza
authors.initials: IM
authors.lastname: Lima
authors.forename: Moacir de Azevedo Bentes
authors.initials: Mde A
authors.lastname: Monteiro Neto
authors.forename: Jairo Kenupp
authors.initials: JK
authors.lastname: Bastos
authors.forename: Ademar Alves
authors.initials: AA
authors.lastname: da Silva Filho
authors.forename: Denise Crispim
authors.initials: DC
authors.lastname: Tavares
mesh_terms: D000818:Animals; D016587:Antimutagenic Agents / Q000494:pharmacology; D000975:Antioxidants / Q000494:pharmacology; D001938:Brazil; D020552:Comet Assay; D006224:Cricetinae; D004249:DNA Damage / Q000187:drug effects*; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects*; D008168:Lung / Q000166:cytology / Q000187:drug effects; D008741:Methyl Methanesulfonate / Q000378:metabolism; D015162:Micronucleus Tests; D010666:Phenylpropionates / Q000494:pharmacology*
chemical_list: D016587:Antimutagenic Agents; D000975:Antioxidants; D010666:Phenylpropionates; C102259:artepillin C; D008741:Methyl Methanesulfonate