title: Detection of an estrogen derivative in two breast cancer cell lines using a single core multimodal probe for imaging (SCoMPI) imaged by a panel of luminescent and vibrational techniques.
abstract: 3-Methoxy-17α-ethynylestradiol or mestranol is a prodrug for ethynylestradiol and the estrogen component of some oral contraceptive formulations. We demonstrate here that a single core multimodal probe for imaging - SCoMPI - can be efficiently grafted onto mestranol allowing its tracking in two breast cancer cell lines, MDA-MB-231 and MCF-7 fixed cells. Correlative imaging studies based on luminescence (synchrotron UV spectromicroscopy, wide field and confocal fluorescence microscopies) and vibrational (AFMIR, synchrotron FTIR spectromicroscopy, synchrotron-based multiple beam FTIR imaging, confocal Raman microspectroscopy) spectroscopies were consistent with one another and showed a Golgi apparatus distribution of the SCoMPI-mestranol conjugate in both cell lines.
authors.affiliation: Ecole Normale Supérieure, Département de chimie, 24, rue Lhomond, 75005 Paris, France. clotilde.policar@ens.fr.
authors.forename: Sylvain
authors.initials: S
authors.lastname: Clède
authors.forename: François
authors.initials: F
authors.lastname: Lambert
authors.forename: Christophe
authors.initials: C
authors.lastname: Sandt
authors.forename: Slavka
authors.initials: S
authors.lastname: Kascakova
authors.forename: Miriam
authors.initials: M
authors.lastname: Unger
authors.forename: Etienne
authors.initials: E
authors.lastname: Harté
authors.forename: Marie-Aude
authors.initials: MA
authors.lastname: Plamont
authors.forename: Rénette
authors.initials: R
authors.lastname: Saint-Fort
authors.forename: Ariane
authors.initials: A
authors.lastname: Deniset-Besseau
authors.forename: Zoher
authors.initials: Z
authors.lastname: Gueroui
authors.forename: Carol
authors.initials: C
authors.lastname: Hirschmugl
authors.forename: Sophie
authors.initials: S
authors.lastname: Lecomte
authors.forename: Alexandre
authors.initials: A
authors.lastname: Dazzi
authors.forename: Anne
authors.initials: A
authors.lastname: Vessières
authors.forename: Clotilde
authors.initials: C
authors.lastname: Policar
mesh_terms: D001943:Breast Neoplasms / Q000473:pathology; D045744:Cell Line, Tumor; D004967:Estrogens / Q000032:analysis*; D005260:Female; D006801:Humans; D008163:Luminescent Measurements / Q000379:methods*; D061986:MCF-7 Cells; D064847:Multimodal Imaging / Q000379:methods*; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods; D014732:Vibration
chemical_list: D004967:Estrogens