title: Crystallization and preliminary X-ray diffraction studies of cyanuric acid hydrolase from Azorhizobium caulinodans.
abstract: Cyanuric acid is synthesized industrially and forms during the microbial metabolism of s-triazine herbicides. Cyanuric acid is metabolized by some microorganisms via cyanuric acid hydrolase (CAH), which opens the s-triazine ring as a prelude to further metabolism. CAH is a member of the rare cyanuric acid hydrolase/barbiturase family. Here, the crystallization and preliminary X-ray diffraction analysis of CAH from Azorhizobium caulinodans are reported. CAH was cocrystallized with barbituric acid, a close analog of cyanuric acid that is a tight-binding competitive inhibitor. Crystals suitable for X-ray diffraction experiments were grown in conditions containing PEG 8K or magnesium sulfate as precipitants. An X-ray diffraction data set was collected from CAH-barbituric acid crystals to 2.7 Å resolution. The crystals were found to belong to space group I4₁22, with unit-cell parameters a = b = 237.9, c = 105.3 Å, α = β = γ = 90°.
authors.affiliation: Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, Minneapolis, MN 55455, USA.
authors.forename: Seunghee
authors.initials: S
authors.lastname: Cho
authors.forename: Ke
authors.initials: K
authors.lastname: Shi
authors.forename: Lawrence P
authors.initials: LP
authors.lastname: Wackett
authors.forename: Hideki
authors.initials: H
authors.lastname: Aihara
mesh_terms: D000581:Amidohydrolases / Q000737:chemistry* / Q000235:genetics; D000595:Amino Acid Sequence; D020599:Azorhizobium caulinodans / Q000201:enzymology* / Q000235:genetics; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics; D003460:Crystallization; D008969:Molecular Sequence Data; D014961:X-Ray Diffraction
chemical_list: D001426:Bacterial Proteins; D000581:Amidohydrolases; C067141:cyanuric acid amidohydrolase
keywords: Azorhizobium caulinodans; barbituric acid; cyanuric acid; cyanuric acid hydrolase; triazine
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