title: PLC-δ1-Lf, a novel N-terminal extended phospholipase C-δ1.
abstract: Phospholipase C-δ (PLC-δ), a key enzyme in phosphoinositide turnover, is involved in a variety of physiological functions. The widely expressed PLC-δ1 isoform is the best characterized and the most well understood phospholipase family member. However, the functional and molecular mechanisms of PLC-δ1 remain obscure. Here, we identified that the N-terminal region of mouse PLC-δ1 gene has two variants, a novel alternative splicing form, named as long form (mPLC-δ1-Lf) and the previously reported short form (mPLC-δ1-Sf), having exon 2 and exon 1, respectively, while both the gene variants share exons 3-16 for RNA transcription. Furthermore, the expression, identification and enzymatic characterization of the two types of PLC-δ1 genes were compared. Expression of mPLC-δ1-Lf was found to be tissue specific, whereas mPLC-δ1-Sf was widely distributed. The recombinant mPLC-δ1-Sf protein exhibited higher activity than recombinant mPLC-δ1-Lf protein. Although, the general catalytic and regulatory properties of mPLC-δ1-Lf are similar to those of PLC-δ1-Sf isozyme, the mPLC-δ1-Lf showed some distinct regulatory properties, such as tissue-specific expression and lipid binding specificity, particularly for phosphatidylserine.
authors.affiliation: Department of Aquatic Life Medicine, Pukyong National University, Busan 608-737, South Korea.
authors.forename: Na Young
authors.initials: NY
authors.lastname: Kim
authors.forename: Sang Jung
authors.initials: SJ
authors.lastname: Ahn
authors.forename: Moo-Sang
authors.initials: MS
authors.lastname: Kim
authors.forename: Jung Soo
authors.initials: JS
authors.lastname: Seo
authors.forename: Bo Seong
authors.initials: BS
authors.lastname: Kim
authors.forename: Hye Jin
authors.initials: HJ
authors.lastname: Bak
authors.forename: Jin Young
authors.initials: JY
authors.lastname: Lee
authors.forename: Myoung-Ae
authors.initials: MA
authors.lastname: Park
authors.forename: Ju Hyeon
authors.initials: JH
authors.lastname: Park
authors.forename: Hyung Ho
authors.initials: HH
authors.lastname: Lee
authors.forename: Joon Ki
authors.initials: JK
authors.lastname: Chung
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002118:Calcium / Q000737:chemistry; D005091:Exons; D005260:Female; D015870:Gene Expression; D006863:Hydrogen-Ion Concentration; D007527:Isoenzymes / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D009928:Organ Specificity; D010718:Phosphatidylserines / Q000737:chemistry; D054803:Phospholipase C delta / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding
chemical_list: D007527:Isoenzymes; D010718:Phosphatidylserines; D054803:Phospholipase C delta; C518025:Plcd1 protein, mouse; D002118:Calcium
keywords: DNA complementary to RNA; Immunohistochemistry; PI-PLC; PLC-δ1; Phosphatidylserine; RT-PCR; SDS–PAGE; cDNA; mPLC-δ1-Lf; reverse transcription polymerase chain reaction; sodium dodecyl sulfate polyacrylamide gel electrophoresis