title: In situ microscopy for in-line monitoring of the enzymatic hydrolysis of cellulose.
abstract: A new in-line method for the monitoring of enzymatic hydrolysis of cellulose is described. Using a new in situ microscope prototype, the noninvasive determination of particle size distributions was possible. For the automated analysis of the acquired images, a new processing algorithm called CelluloseAnalyzer was developed. It enabled tracking of the number of particles and moreover allowed monitoring of the proportions of particle size fractions during the course of enzymatic hydrolysis reactions. Using this technique, significant differences between hydrolysis with endoglucanases and cellulase mixtures were observed. Furthermore, the in situ microscopy results were compared with results from off-line measurements with laser diffraction spectroscopy and gel permeation chromatography.
authors.affiliation: Institute of Technical Biocatalysis, Hamburg University of Technology, Denickestrasse 15, 21073 Hamburg, Germany.
authors.forename: Britta
authors.initials: B
authors.lastname: Opitz
authors.forename: Andreas
authors.initials: A
authors.lastname: Prediger
authors.forename: Christian
authors.initials: C
authors.lastname: Lüder
authors.forename: Marrit
authors.initials: M
authors.lastname: Eckstein
authors.forename: Lutz
authors.initials: L
authors.lastname: Hilterhaus
authors.forename: Patrick
authors.initials: P
authors.lastname: Lindner
authors.forename: Sascha
authors.initials: S
authors.lastname: Beutel
authors.forename: Thomas
authors.initials: T
authors.lastname: Scheper
authors.forename: Andreas
authors.initials: A
authors.lastname: Liese
mesh_terms: D002482:Cellulose / Q000378:metabolism*; D002850:Chromatography, Gel / Q000379:methods; D057075:Enzyme Assays / Q000379:methods*; D006868:Hydrolysis; D008853:Microscopy / Q000379:methods*
chemical_list: D002482:Cellulose