title: [Effects of CXCR7-shRNA lentiviral vector on the growth and invasiveness of human hepatoma carcinoma cells in vitro].
abstract: OBJECTIVE
  To explore the effects of CXCR7 knock-down by CXCR7-shRNA lentiviral vector on the proliferation and invasion of human hepatoma carcinoma cells in vitro.
  
  
  METHODS
  CXCR7-shRNA lentiviral vector was transfected into hepatocellular carcinoma HCCLM3 cells. The changes in mRNA and protein expression of CXCR7 in the transfected cells were investigated using real-time PCR and Western blotting, respectively, and MTT assay was employed to assess the cell proliferation changes. In vitro adhesion assay and transwell chamber test were used to observe the adhesion and invasiveness of HCCLM3 cells, respectively.
  
  
  RESULTS
  Transfection of HCCLM3 cells with CXCR7-shRNA lentiviral vector resulted in a significantly decreased expression of CXCR7 at both mRNA and protein levels (P<0.01) and obvious suppression of the cell proliferative activity (P<0.05). CXCR7-shRNA also significantly suppressed the invasiveness and adhesion of HCCLM3 cells (P<0.01).
  
  
  CONCLUSIONS
  CXCR7 knock-down can significantly inhibit the proliferation and invasiveness of human hepatoma carcinoma cells in vitro, suggesting the value of CXCR7 as a potential target for hepatoma carcinoma therapy.
authors.affiliation: School of Biomedicine, Chengdu Medical College, Chengdu, China. xiaozhendai@yahoo.com.cn
authors.forename: Xiaozhen
authors.initials: X
authors.lastname: Dai
authors.forename: Xin
authors.initials: X
authors.lastname: Xiong
authors.forename: Lan
authors.initials: L
authors.lastname: Wang
authors.forename: Kejian
authors.initials: K
authors.lastname: Pan
authors.forename: Lang
authors.initials: L
authors.lastname: He
authors.forename: Hong
authors.initials: H
authors.lastname: Li
mesh_terms: D006528:Carcinoma, Hepatocellular / Q000235:genetics / Q000473:pathology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D005822:Genetic Vectors; D006801:Humans; D016086:Lentivirus / Q000235:genetics; D008113:Liver Neoplasms / Q000235:genetics / Q000473:pathology*; D034741:RNA, Small Interfering / Q000235:genetics; D054387:Receptors, CXCR / Q000235:genetics*; D014162:Transfection
chemical_list: C501896:ACKR3 protein, human; D034741:RNA, Small Interfering; D054387:Receptors, CXCR