title: Partial genetic characterization of Sedlec virus (Orthobunyavirus, Bunyaviridae).
abstract: Sedlec virus (SEDV) was isolated from the blood of a reed warbler (Acrocephalus scirpaceus) in July 1984 in South Moravia, Czech Republic. In this study first genetic data of SEDV are presented which allow an estimate on its phylogenetic and taxonomic positioning within the genus Orthobunyavirus. The phylogenetic analysis of a 369 nt long stretch within the S segment (nucleocapsid protein gene and non-structural S protein gene) indicates genetic relatedness of SEDV to Leanyer virus and Simbu group viruses, while the phylogenetic tree based on 1796nt long sequences of the L segment (RNA-dependent RNA polymerase gene) demonstrates genetic relationship of SEDV to two yet unclassified orthobunyaviruses: I612045 virus (isolated in India in 1961) and Oyo virus (isolated in Nigeria in 1964). Considering the genetic distances and the phylogenetic analyses, SEDV might represent a novel serogroup of the Orthobunyavirus genus.
authors.affiliation: Department of Microbiology and Infectious Diseases, Faculty of Veterinary Science, Szent István University, Hungária krt. 23-25, H-1143 Budapest, Hungary; Viral Zoonoses, Emerging and Vector-Borne Infections Group, Institute of Virology, University of Veterinary Medicine Vienna, Veterinaerplatz 1, 1210 Vienna, Austria. Electronic address: Tamas.Bakonyi@vetmeduni.ac.at.
authors.forename: Tamás
authors.initials: T
authors.lastname: Bakonyi
authors.forename: Jolanta
authors.initials: J
authors.lastname: Kolodziejek
authors.forename: Ivo
authors.initials: I
authors.lastname: Rudolf
authors.forename: Rebeka
authors.initials: R
authors.lastname: Berčič
authors.forename: Norbert
authors.initials: N
authors.lastname: Nowotny
authors.forename: Zdenek
authors.initials: Z
authors.lastname: Hubálek
mesh_terms: D000818:Animals; D002044:Bunyaviridae Infections / Q000821:virology; D018153:Czech Republic; D051379:Mice; D016851:Orthobunyavirus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics; D020308:Songbirds / Q000821:virology; D014776:Virus Cultivation
chemical_list: D012367:RNA, Viral
keywords: Acrocephalus; Leanyer virus; Orthobunyavirus; Sedlec virus; Simbu group