title: Characterization of polysaccharides with marked inhibitory effect on lipid accumulation in Pleurotus eryngii.
abstract: Mushrooms have a great potential for the production of useful bioactive metabolites. To explore the bioactive compounds from edible mushrooms for interfering with the development of macrophage-derived foam cells, which is recognized as the hallmark of early atherosclerosis, eight types of mushrooms polysaccharides had been selected to be tested. Consequently, different mushrooms polysaccharides displayed diverse component profiles. Of polysaccharides that we tested, the Pleurotus eryngii polysaccharide had the strongest inhibitory effect on lipid accumulation. Furthermore, through fractionation of DEAE-52 and Sephadex G-100, the polysaccharide from P. eryngii had been successfully purified and identified. By the analysis of IR, GC, and HPLC, the purified polysaccharide was estimated to be 30-38 kDa for the average molecular weight with the monosaccharide composition mainly composed of D-types of mannose, glucose and galactose. Findings presented in this report firstly provide direct evidence, which links the purified polysaccharide moiety with the biological function in foam-cell model.
authors.affiliation: Jiangsu Key Laboratory for Microbes and Functional Genomics, Jiangsu Engineering and Technology Research Center for Microbiology, China.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Chen
authors.forename: Yangyang
authors.initials: Y
authors.lastname: Yong
authors.forename: Meichun
authors.initials: M
authors.lastname: Xing
authors.forename: Yifan
authors.initials: Y
authors.lastname: Gu
authors.forename: Zhao
authors.initials: Z
authors.lastname: Zhang
authors.forename: Shizhu
authors.initials: S
authors.lastname: Zhang
authors.forename: Ling
authors.initials: L
authors.lastname: Lu
mesh_terms: D000818:Animals; D002460:Cell Line; D002851:Chromatography, High Pressure Liquid; D005487:Foam Cells / Q000187:drug effects / Q000378:metabolism; D048690:Fruiting Bodies, Fungal / Q000737:chemistry; D050356:Lipid Metabolism / Q000187:drug effects*; D008077:Lipoproteins, LDL / Q000494:pharmacology; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D008970:Molecular Weight; D009005:Monosaccharides / Q000032:analysis; D020076:Pleurotus / Q000737:chemistry*; D011134:Polysaccharides / Q000494:pharmacology*; D013055:Spectrophotometry, Infrared
chemical_list: D008077:Lipoproteins, LDL; D009005:Monosaccharides; D011134:Polysaccharides; C099252:oxidized low density lipoprotein
keywords: GC; HPLC; IR; Lipid accumulation; Macrophage-derived foam cell; Mushroom; NMR; PEPE; Pleurotus eryngii; Polysaccharide; gas chromatography; high-performance liquid chromatography; infrared; nuclear magnetic resonance; oxLDL; oxidized low density lipoprotein; polysaccharide from P. eryngii fruiting body