title: Structural elucidation of polysaccharide fractions from brown seaweed Sargassum pallidum.
abstract: The structural characteristics of two purified fractions of polysaccharides from Sargassum pallidum (SPS) were investigated in the present study. As results, the molecular weights of the two polysaccharide fractions, SPS-3-1 and SPS-3-2, were determined to be 5.87 and 7.25 kDa, respectively. SPS-3-1 was composed of glucose, mannose and galactose in a molar ratio of 11.18:1.00:0.96, while SPS-3-2 was composed of fucose, xylose, mannose, glucose and galactose in a molar ratio of 2.53:0.61:1.00:0.46:0.92. Both SPS-3-1 and SPS-3-2 exhibited the characteristics of polysaccharide in the frequency range of 4000-400 cm(-1) based on their Fourier-transform infrared spectra. Furthermore, the results of periodic acid oxidation, Smith degradation, methylation analysis and nuclear magnetic resonance spectroscopic analysis suggested that SPS-3-2 was composed of (1→4)-linked fucopyranosyl backbone and (1→3)-linked galactopyranosyl, (1→3)-linked mannopyranosyl, (1→2)-linked xylopyranosyl and (1→6)-linked glucopyranosyl branch chains.
authors.affiliation: College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Hong
authors.initials: H
authors.lastname: Ye
authors.forename: Chunhong
authors.initials: C
authors.lastname: Zhou
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.forename: Bing
authors.initials: B
authors.lastname: Hu
authors.forename: Xiaoqing
authors.initials: X
authors.lastname: Wang
authors.forename: Xiaoxiong
authors.initials: X
authors.lastname: Zeng
mesh_terms: D005591:Chemical Fractionation; D002851:Chromatography, High Pressure Liquid; D008401:Gas Chromatography-Mass Spectrometry; D009682:Magnetic Resonance Spectroscopy; D009005:Monosaccharides / Q000032:analysis; D011134:Polysaccharides / Q000737:chemistry*; D044647:Sargassum / Q000737:chemistry*; D012624:Seaweed / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D009005:Monosaccharides; D011134:Polysaccharides
keywords: Characterization; Polysaccharide; Sargassum pallidum; Structure