title: Genetic polymorphisms in Matrix Metalloproteinases -1 and -7 and susceptibility to gastric cancer: an association study and meta-analysis.
abstract: Matrix Metalloproteinases (MMPs) play an important role in gastric cancer (GC). Accumulated evidence suggests that functional MMP-1 and MMP-7 gene polymorphisms are associated with several tumors. The aim of this study was to investigate two single nucleotide polymorphisms, MMP-1 -1607 1G/2G and MMP-7 -181 A/G, and their potential relationship with GC. We examined 246 GC patients and 252 age-and sex-matched controls from Sichuan province in China. Genotypes were determined using a polymerase chain reaction-restriction fragment length polymorphism strategy and DNA sequencing. We also performed a meta-analysis of relevant studies, involving 1084 cases and 1721 controls, to place our findings in a broader context. No significant relationship was observed between the MMP-1 -1607 1G/2G alleles and genotypes and the risk of GC. There were significant differences in the genotypes and allele distributions of the -181 A/G polymorphism of the MMP-7 gene between cases and controls. The -181 A allele carriers had a significantly increased risk of GC compared with -181 G allele carriers (OR=3.051, 95% CI, 1.475-6.310, P=0.002), and the AA genotype of -181 A/G was associated with an increased risk of GC compared with the AG genotype (OR=3.189, 95% CI, 1.523-6.676, P=0.001). A meta-analysis of six studies also showed a significant risk of GC associated with MMP-7 polymorphism.
authors.affiliation: Central Laboratory of Clinical College, Anhui Medical University, Hefei 230601, P. R. China. fangxiaokai@126.com.
authors.forename: Wen-Liang
authors.initials: WL
authors.lastname: Fang
authors.forename: Wei-Bo
authors.initials: WB
authors.lastname: Liang
authors.forename: Lin-Bo
authors.initials: LB
authors.lastname: Gao
authors.forename: Bin
authors.initials: B
authors.lastname: Zhou
authors.forename: Feng-Li
authors.initials: FL
authors.lastname: Xiao
authors.forename: Lin
authors.initials: L
authors.lastname: Zhang
mesh_terms: D000328:Adult; D000368:Aged; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D008297:Male; D020781:Matrix Metalloproteinase 1 / Q000235:genetics*; D020783:Matrix Metalloproteinase 7 / Q000235:genetics*; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D013274:Stomach Neoplasms / Q000201:enzymology / Q000235:genetics*
chemical_list: C060932:MMP7 protein, human; D020783:Matrix Metalloproteinase 7; C522360:MMP1 protein, human; D020781:Matrix Metalloproteinase 1