title: Short communication: quantitative comparison of iodothyronine deiodinase I and II mRNA expression in ovine tissues.
abstract: Iodothyronine deiodinases I and II (DIO1 and DIO2) remove iodine from T4 to convert it to a more biologically active T3. The relative contribution of different tissue deiodinases to the establishment of a euthyroid state in sheep is not known. The objective of this study was to quantitate the amounts of transcription of DIO1 and DIO2 deiodinases in different ovine tissues. Using RT-qPCR, we found that DIO1 deiodinase is transcribed in skeletal muscle, kidney, and heart, more than thyroid, in diaphragm in quantities very similar to thyroid, and in liver, spleen, lung, and mammary gland lower than thyroid. We also found that the level of DIO2 transcription in all other tissues was lower than that in thyroid. In clinical settings, measurement of DIO1 and DIO2 expression in a given tissue may provide important clues on the intensity of selenium deficiency and its effects on the metabolism of thyroid hormones.
authors.affiliation: Department of Basic Science, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad 91779-48974, Iran. Electronic address: mohamadj_foroughi@yahoo.com.
authors.forename: Mohammad Ali
authors.initials: MA
authors.lastname: Foroughi
authors.forename: Hesam
authors.initials: H
authors.lastname: Dehghani
mesh_terms: D000818:Animals; D007453:Iodide Peroxidase / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D007668:Kidney / Q000378:metabolism; D018482:Muscle, Skeletal / Q000378:metabolism; D009206:Myocardium / Q000378:metabolism; D012333:RNA, Messenger / Q000737:chemistry / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary; D012756:Sheep / Q000378:metabolism; D014018:Tissue Distribution; D000097564:Iodothyronine Deiodinase Type II
chemical_list: D007453:Iodide Peroxidase; D012333:RNA, Messenger; D000097564:Iodothyronine Deiodinase Type II; C109812:iodothyronine deiodinase type I
keywords: 5′mono deiodinase type I; 5′mono deiodinase type II; DIO1; DIO2; Iodothyronine deiodinases; RT-qPCR; Sheep; Tissue expression; Transcription; real-time reverse transcription polymerase chain reaction