title: Three-dimensional structure of RBcel1, a metagenome-derived psychrotolerant family GH5 endoglucanase.
abstract: RBcel1 is an endoglucanase belonging to glycoside hydrolase family 5 subfamily 5 (GH5_5) that was recently identified from a soil metagenome library from the Antarctic. Unlike its closest structural homologue (Cel5A from Thermoascus aurantiacus), this enzyme was reported to be able to catalyze transglycosylation reactions and has putatively been implicated in the bacterial cellulose-synthesis process. Here, the structure of RBcel1 at 1.4 Å resolution, solved by molecular replacement, is reported. The structure and putative substrate-binding site are described and compared with those of other GH5_5 subfamily members.
authors.affiliation: Centre d'Ingénierie des Protéines, Laboratoire de Macromolécules Biologiques, Université de Liège (ULg), Bâtiment B6, Allée de la Chimie 3, 4000 Liège, Belgium.
authors.forename: Maud
authors.initials: M
authors.lastname: Delsaute
authors.forename: Renaud
authors.initials: R
authors.lastname: Berlemont
authors.forename: Dominique
authors.initials: D
authors.lastname: Dehareng
authors.forename: Dany
authors.initials: D
authors.lastname: Van Elder
authors.forename: Moreno
authors.initials: M
authors.lastname: Galleni
authors.forename: Cédric
authors.initials: C
authors.lastname: Bauvois
mesh_terms: D000595:Amino Acid Sequence; D001665:Binding Sites / Q000502:physiology; D002480:Cellulase / Q000737:chemistry* / Q000235:genetics*; D003460:Crystallization; D006026:Glycoside Hydrolases / Q000737:chemistry* / Q000235:genetics*; D054892:Metagenome / Q000235:genetics*; D008968:Molecular Conformation; D008969:Molecular Sequence Data; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary
chemical_list: D006026:Glycoside Hydrolases; D002480:Cellulase
keywords: bacterial cellulose synthesis; endoglucanases; family 5 glycosyl hydrolases; psychrotolerant; uncultured bacteria
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