title: Fatty acid remodeling in cellular glycerophospholipids following the activation of human T cells.
abstract: Changes in fatty acid (FA) and glycerophospholipid (GPL) metabolism associated with cell cycle entry are not fully understood. In this study FA-GPL remodeling was investigated in resting and proliferating primary human T cells. Significant changes were measured in the composition and distribution of FAs in GPLs following receptor activation of human T cells. The FA distribution of proliferating T cells was very similar to that of the human Jurkat T cell line and when the stimulus was removed from proliferating T cells, they stopped proliferating and the FA distribution largely reverted back to that of resting T cells. The cellular content of saturated and monounsaturated FAs was significantly increased in proliferating cells, which was associated with an induction of FA synthase and stearoyl-CoA desaturase-1 gene expression. Additionally, cellular arachidonate was redistributed in GPLs in a distinct pattern that was unlike any other FAs. This redistribution was associated with an induction of CoA-dependent and CoA-independent remodeling. Accordingly, significant changes in the expression of several acyl-CoA synthetases, lysophospholipid acyltransferases, and phospholipase A2 were measured. Overall, these results suggest that metabolic pathways are activated in proliferating T cells that may represent fundamental changes associated with human cell proliferation.
authors.affiliation: Department of Chemistry and Biochemistry, Université de Moncton, Moncton, NB E1A 3E9, Canada; and.
authors.forename: Philippe Pierre
authors.initials: PP
authors.lastname: Robichaud
authors.forename: Katherine
authors.initials: K
authors.lastname: Boulay
authors.forename: Jean Éric
authors.initials: JÉ
authors.lastname: Munganyiki
authors.forename: Marc E
authors.initials: ME
authors.lastname: Surette
mesh_terms: D001095:Arachidonic Acids / Q000378:metabolism*; D002478:Cells, Cultured; D054890:Fatty Acid Synthase, Type I / Q000235:genetics / Q000378:metabolism; D005227:Fatty Acids / Q000378:metabolism; D015870:Gene Expression; D020404:Glycerophospholipids / Q000378:metabolism*; D006801:Humans; D008213:Lymphocyte Activation; D013230:Stearoyl-CoA Desaturase / Q000235:genetics / Q000378:metabolism; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism*
chemical_list: D001095:Arachidonic Acids; D005227:Fatty Acids; D020404:Glycerophospholipids; C523776:SCD1 protein, human; D013230:Stearoyl-CoA Desaturase; C518729:FASN protein, human; D054890:Fatty Acid Synthase, Type I
keywords: acyl-CoA synthetase; arachidonic acid; fatty acid synthase; lysophospholipid acyltransferase; phospholipase A2; stearoyl-CoA desaturase 1
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