title: Transcriptomic profiling in childhood H1N1/09 influenza reveals reduced expression of protein synthesis genes.
abstract: We compared the blood RNA transcriptome of children hospitalized with influenza A H1N1/09, respiratory syncytial virus (RSV) or bacterial infection, and healthy controls. Compared to controls, H1N1/09 patients showed increased expression of inflammatory pathway genes and reduced expression of adaptive immune pathway genes. This was validated on an independent cohort. The most significant function distinguishing H1N1/09 patients from controls was protein synthesis, with reduced gene expression. Reduced expression of protein synthesis genes also characterized the H1N1/09 expression profile compared to children with RSV and bacterial infection, suggesting that this is a key component of the pathophysiological response in children hospitalized with H1N1/09 infection.
authors.affiliation: Section of Paediatrics.
authors.forename: Jethro A
authors.initials: JA
authors.lastname: Herberg
authors.forename: Myrsini
authors.initials: M
authors.lastname: Kaforou
authors.forename: Stuart
authors.initials: S
authors.lastname: Gormley
authors.forename: Edward R
authors.initials: ER
authors.lastname: Sumner
authors.forename: Sanjay
authors.initials: S
authors.lastname: Patel
authors.forename: Kelsey D J
authors.initials: KD
authors.lastname: Jones
authors.forename: Stéphane
authors.initials: S
authors.lastname: Paulus
authors.forename: Colin
authors.initials: C
authors.lastname: Fink
authors.forename: Federico
authors.initials: F
authors.lastname: Martinon-Torres
authors.forename: Giovanni
authors.initials: G
authors.lastname: Montana
authors.forename: Victoria J
authors.initials: VJ
authors.lastname: Wright
authors.forename: Michael
authors.initials: M
authors.lastname: Levin
mesh_terms: D000293:Adolescent; D001424:Bacterial Infections / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D002648:Child; D016000:Cluster Analysis; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype; D007251:Influenza, Human / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D014176:Protein Biosynthesis / Q000235:genetics*; D015203:Reproducibility of Results; D018357:Respiratory Syncytial Virus Infections / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D018113:Respiratory Syncytial Virus, Human; D015398:Signal Transduction
keywords: Peptide Chain Initiation; RSV; eIF-2 Kinase; gene expression profiling; influenza; microarray analysis; pediatric; respiratory tract infection
references.citation: Crit Care. 2010;14(5):R167
references.pmid: 20840779
references.citation: Nature. 2012 Mar 25;484(7395):519-23
references.pmid: 22446628
references.citation: Pediatr Infect Dis J. 2011 May;30(5):438-40
references.pmid: 21102362
references.citation: PLoS Genet. 2011 Aug;7(8):e1002234
references.pmid: 21901105
references.citation: Cell Cycle. 2008 May 1;7(9):1146-50
references.pmid: 18418049
references.citation: Mol Cell Biol. 1982 Dec;2(12):1644-8
references.pmid: 14582206
references.citation: Nature. 2010 Nov 18;468(7322):452-6
references.pmid: 21085181
references.citation: J Genet Genomics. 2011 Nov 20;38(11):533-7
references.pmid: 22133684
references.citation: EMBO J. 2000 Dec 15;19(24):6891-9
references.pmid: 11118224
references.citation: J Biol Chem. 2006 Nov 10;281(45):34064-71
references.pmid: 16973618
references.citation: Crit Care. 2012 Aug 16;16(4):R157
references.pmid: 22898401
references.citation: Pediatr Res. 2011 Jun;69(6):473-8
references.pmid: 21364490
references.citation: Virol J. 2011 Mar 25;8:140
references.pmid: 21439068
references.citation: Blood. 2007 Mar 1;109(5):2066-77
references.pmid: 17105821
references.citation: J Gen Virol. 2013 Jan;94(Pt 1):59-68
references.pmid: 23052390