title: Affinity flow fractionation of cells via transient interactions with asymmetric molecular patterns.
abstract: Flow fractionation of cells using physical fields to achieve lateral displacement finds wide applications, but its extension to surface molecule-specific separation requires labeling. Here we demonstrate affinity flow fractionation (AFF) where weak, short-range interactions with asymmetric molecular patterns laterally displace cells in a continuous, label-free process. We show that AFF can directly draw neutrophils out of a continuously flowing stream of blood with an unprecedented 400,000-fold depletion of red blood cells, with the sorted cells being highly viable, unactivated, and functionally intact. The lack of background erythrocytes enabled the use of AFF for direct enumeration of neutrophils by a downstream detector, which could distinguish the activation state of neutrophils in blood. The compatibility of AFF with capillary microfluidics and its ability to directly separate cells with high purity and minimal sample preparation will facilitate the design of simple and portable devices for point-of-care diagnostics and quick, cost-effective laboratory analysis.
authors.affiliation: Department of Mechanical Engineering, Massachusetts Institute of Technology, MA 02139, USA.
authors.forename: Suman
authors.initials: S
authors.lastname: Bose
authors.forename: Rishi
authors.initials: R
authors.lastname: Singh
authors.forename: Mikhail
authors.initials: M
authors.lastname: Hanewich-Hollatz
authors.forename: Chong
authors.initials: C
authors.lastname: Shen
authors.forename: Chia-Hua
authors.initials: CH
authors.lastname: Lee
authors.forename: David M
authors.initials: DM
authors.lastname: Dorfman
authors.forename: Jeffrey M
authors.initials: JM
authors.lastname: Karp
authors.forename: Rohit
authors.initials: R
authors.lastname: Karnik
mesh_terms: D001798:Blood Proteins / Q000378:metabolism*; D002458:Cell Fractionation / Q000379:methods*; D002478:Cells, Cultured; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D044085:Microfluidics / Q000379:methods*; D009504:Neutrophils / Q000166:cytology* / Q000502:physiology*
chemical_list: D001798:Blood Proteins
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