title: The current status of laboratory diagnosis of Angiostrongylus cantonensis infections in humans using serologic and molecular methods.
abstract: Laboratory diagnosis of angiostrongyliasis relies on serological techniques, since definitive diagnosis is insensitive. Modern antibody detection methods focus on antibodies to the 29 and 31 kDa proteins of the parasite. Antigen detection may ultimately prove to be more reliable than antibody detection but no method has been adopted for clinical diagnostic use. Diagnosis using PCR amplification of DNA sequences specific to Angiostrongylus cantonensis have been developed but have not yet been validated for clinical use. Diagnostic tests have not been developed commercially and in the United States tests developed experimentally by non-commercial laboratories have to be approved by the Food and Drug Administration before they can be sold to other laboratories for diagnostic purposes.
authors.affiliation: Division of Parasitic Diseases & Malaria, Centers for Disease Control & Prevention, Atlanta, GA 30329 , USA. pwilkins@cdc.gov
authors.forename: Patricia P
authors.initials: PP
authors.lastname: Wilkins
authors.forename: Yvonne
authors.initials: Y
authors.lastname: Qvarnstrom
authors.forename: A Christian
authors.initials: AC
authors.lastname: Whelen
authors.forename: Caitlin
authors.initials: C
authors.lastname: Saucier
authors.forename: Alexandre J
authors.initials: AJ
authors.lastname: da Silva
authors.forename: Praphathip
authors.initials: P
authors.lastname: Eamsobhana
mesh_terms: D017185:Angiostrongylus cantonensis / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification*; D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood* / Q000134:cerebrospinal fluid; D000947:Antigens, Helminth / Q000097:blood* / Q000134:cerebrospinal fluid; D004247:DNA / Q000134:cerebrospinal fluid; D057069:Diagnostic Test Approval; D006801:Humans; D016133:Polymerase Chain Reaction; D012698:Serologic Tests; D017206:Strongylida Infections / Q000175:diagnosis*; D014481:United States; D014486:United States Food and Drug Administration
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D004247:DNA
keywords: Angiostrongyliasis; Diagnosis; Eosinophilic meningitis; Molecular methods; Serological methods
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