title: A high-sensitive and non-radioisotopic fluorescence dye method for evaluating bacterial adhesion to denture materials.
abstract: Oral bacteria adhered to dental material surfaces are known to cause various oral diseases. This study aimed to develop a highsensitive and non-radioisotopic fluorescence dye method for quantification of oral bacteria (Streptococcus, Actinomyces and Veillonella) adhered to denture material surfaces. The amount of adhered bacteria was estimated from the fluorescence intensity derived from resazurin, which is reduced by bacterial metabolic reactions. The addition of bacterial metabolic substrates (glucose for Streptococcus and Actinomyces and sodium lactate for Veillonella) to the reaction mixture increased the fluorescence intensity by 2.3-110 times, subsequently improved the sensitivity. Furthermore, an experimental device having silicon wells containing test material was carefully designed for accurate quantification of bacteria adhered to test materials. The improved resazurin method using a new experimental device successfully enabled the quantification of bacterial adhesion to polymethyl methacrylate and other three conventional denture materials.
authors.affiliation: Division of Oral Ecology and Biochemistry Tohoku University Graduate School of Dentistry, 4-1 Seiryo-machi, Aoba-ku, Sendai 980-8575 Japan.
authors.forename: Yoko
authors.initials: Y
authors.lastname: Sakuma
authors.forename: Jumpei
authors.initials: J
authors.lastname: Washio
authors.forename: Keiichi
authors.initials: K
authors.lastname: Sasaki
authors.forename: Nobuhiro
authors.initials: N
authors.lastname: Takahashi
mesh_terms: D000190:Actinomyces / Q000302:isolation & purification / Q000378:metabolism; D000328:Adult; D001419:Bacteria / Q000302:isolation & purification*; D001422:Bacterial Adhesion; D003764:Dental Materials / Q000737:chemistry*; D003778:Dentures / Q000382:microbiology*; D005260:Female; D005456:Fluorescent Dyes; D005947:Glucose / Q000378:metabolism; D006801:Humans; D010078:Oxazines / Q000378:metabolism; D010084:Oxidation-Reduction; D019904:Polymethyl Methacrylate / Q000737:chemistry; D012463:Saliva / Q000382:microbiology / Q000502:physiology; D019354:Sodium Lactate / Q000378:metabolism; D013295:Streptococcus mutans / Q000302:isolation & purification / Q000378:metabolism; D013298:Streptococcus sanguis / Q000302:isolation & purification / Q000378:metabolism; D013499:Surface Properties; D014678:Veillonella / Q000302:isolation & purification / Q000378:metabolism; D014966:Xanthenes
chemical_list: D003764:Dental Materials; D005456:Fluorescent Dyes; D010078:Oxazines; D014966:Xanthenes; C020456:phenoxazinone; C005843:resazurin; D019904:Polymethyl Methacrylate; D005947:Glucose; D019354:Sodium Lactate