title: Labeling primary amine groups in peptides and proteins with N-hydroxysuccinimidyl ester modified Fe3O4@SiO2 nanoparticles containing cleavable disulfide-bond linkers.
abstract: The surface of superparamagnetic silica coated iron oxide (Fe3O4@SiO2) nanoparticles was functionalized with a disulfide bond linked N-hydroxysuccinimidyl (NHS) ester group in order to develop a method for labeling primary amines in peptides/proteins. The nanoparticle labeled proteins/peptides formed after NHS ester reaction with the primary amine groups were isolated using a magnet without any additional purification step. Nanoparticle moieties conjugated to peptides/proteins were then trimmed by cleavage at the disulfide linker with a reducing agent. The labeled peptides were analyzed by LC-MS/MS to determine their sequences and the sites of NHS ester labeling. This novel approach allowed characterization of lysine residues on the solvent accessible surface of native bovine serum albumin. Low cost, rapid magnetic separation, and specificity toward primary amine groups make NHS ester coated Fe3O4@SiO2 nanoparticles a potential labeling probe to study proteins on living cell surfaces.
authors.affiliation: Department of Chemistry and ‡Advanced Materials Research Institute, University of New Orleans , 2000 Lakeshore Drive, New Orleans, Louisiana 70148, United States.
authors.forename: Ujwal S
authors.initials: US
authors.lastname: Patil
authors.forename: Haiou
authors.initials: H
authors.lastname: Qu
authors.forename: Daniela
authors.initials: D
authors.lastname: Caruntu
authors.forename: Charles J
authors.initials: CJ
authors.lastname: O'Connor
authors.forename: Arjun
authors.initials: A
authors.lastname: Sharma
authors.forename: Yang
authors.initials: Y
authors.lastname: Cai
authors.forename: Matthew A
authors.initials: MA
authors.lastname: Tarr
mesh_terms: D000588:Amines / Q000032:analysis*; D000818:Animals; D002417:Cattle; D002853:Chromatography, Liquid / Q000379:methods; D004220:Disulfides / Q000737:chemistry; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D008958:Models, Molecular; D010455:Peptides / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry; D013194:Staining and Labeling / Q000379:methods; D013388:Succinimides / Q000737:chemistry*; D053719:Tandem Mass Spectrometry / Q000379:methods
chemical_list: D000588:Amines; D004220:Disulfides; D058185:Magnetite Nanoparticles; D010455:Peptides; D013388:Succinimides; D012710:Serum Albumin, Bovine; D012822:Silicon Dioxide
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