title: [Xuefu zhuyu oral liquid intervened stress-stimulated depression model rats].
abstract: OBJECTIVE
  To observe effects of xuefu zhuyu Oral Liquid (XZOL) on the brain behavior and monoamine neurotransmitter 5-HT, and brain derived neurotrophic factor (BDNF) content on depression model rats.
  
  
  METHODS
  Male SD rats were randomly divided into the control group, the model group, the XZOL group, and the Deanxit Tablet group, 12 in each group. The depressive rat model was established by chronic unpredictable mild stress method. XZOL was administered to rats in the XZOL group by gastro-gavage, while Deanxit Tablet was given to those in the Deanxit Tablet group by gastrogavage. The intervention lasted for two weeks. The behavioral changes were observed by sucrose water consumption test and open-field test. The 5-HT and BDNF contents were detected using ELISA.
  
  
  RESULTS
  After chronic stress stimulus, experimental rats in the model group might have abnormal behavioral changes and lowered 5-HT content, showing statistical difference when compared with the control group (P <0.01). No obvious change in stimulated rats' behavior after intervention of XZOL and Deanxit Tablet. 5-HT content was not obviously reduced (P>0.05). Besides, XZOL was superior to Deanxit Tablet in increasing the 5-HT content (P<0.05). But the brain BDNF level of rats in the model group was not statistically different from that of rats in the model group (P >0.05), while the brain BDNF level of rats in the XZOL group and the Deanxit Tablet group was lower than that of rats in the model group (P <0.01).
  
  
  CONCLUSIONS
  Stress can lead to behavioral changes and lowered 5-HT content of rats. The intervention of XZOL could fight against depression-induced behavioral changes and increase 5-HT content. But it did not significantly affect the brain BDNF level. We inferred that it might not effect through the BDNF pathway.
authors.affiliation: Department of Traditional Chinese Medicine, Ruijin Hospital, Medical College of Shanghai Jiaotong University, Shanghai 200025, China
authors.forename: Ling-ling
authors.initials: LL
authors.lastname: Lu
authors.forename: Xiao-Heng
authors.initials: XH
authors.lastname: Shen
authors.forename: Jing-xian
authors.initials: JX
authors.lastname: Chen
mesh_terms: D000818:Animals; D001522:Behavior, Animal; D001921:Brain / Q000187:drug effects / Q000378:metabolism; D019208:Brain-Derived Neurotrophic Factor / Q000378:metabolism; D003863:Depression / Q000188:drug therapy*; D004195:Disease Models, Animal; D004365:Drugs, Chinese Herbal / Q000627:therapeutic use*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D012701:Serotonin / Q000378:metabolism; D013315:Stress, Psychological / Q000378:metabolism*
chemical_list: D019208:Brain-Derived Neurotrophic Factor; D004365:Drugs, Chinese Herbal; C513826:Xue-Fu-Zhu-Yu decoction; D012701:Serotonin