title: Genome wide microarray based expression profiles during early embryogenesis in diapause induced and non-diapause eggs of polyvoltine silkworm Bombyx mori.
abstract: Diapause was induced in polyvoltine silkworm B. mori eggs and the molecular mechanism involved in diapause was investigated using a genome wide microarray. In diapause eggs, 638 and 675 genes were upregulated, while, in non-diapause eggs 1136 and 595 genes were upregulated at 18 h and 30 h, respectively after oviposition. Real-time qPCR analysis confirmed the expression of 20 genes, and the relative expression levels of the Aquaporin gene was highest among the 20 genes, followed by Sorbitol dehydrogenase-2 and Cytochrome b5 in diapause eggs, while, Kruppel homolog, Period and Relish were higher in non-diapause eggs. The upregulation of SDH-2 and cytochrome b5 indicates increased metabolic rate in diapause-destined embryos prior to the onset of diapause within 36 h as a preparatory phase. This study provides an insight into the early molecular events for the induction and maintenance of diapause in B. mori.
authors.affiliation: Genomics Division, Seribiotech Research Laboratory, Carmelaram Post, Kodathi, Bangalore, 560 035, India.
authors.forename: Sirigineedi
authors.initials: S
authors.lastname: Sasibhushan
authors.forename: Rao
authors.initials: R
authors.lastname: C G P
authors.forename: Kangayam M
authors.initials: KM
authors.lastname: Ponnuvel
mesh_terms: D000818:Animals; D020346:Aquaporins / Q000235:genetics; D012831:Bombyx / Q000235:genetics* / Q000254:growth & development*; D015786:Cytochromes b5 / Q000235:genetics; D064666:Diapause, Insect / Q000235:genetics*; D004625:Embryo, Nonmammalian; D047108:Embryonic Development / Q000235:genetics; D005260:Female; D018507:Gene Expression Regulation, Developmental; D017344:Genes, Insect; D049750:Genome, Insect; D019476:Insect Proteins / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis; D010058:Oviposition / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D020346:Aquaporins; D019476:Insect Proteins; D015786:Cytochromes b5
keywords: Bombyx mori; Diapause; Gene expression; Microarray