title: Ovarian cyst fluids are a cache of tumor biomarkers that include calgranulin A and calgranulin B isoforms.
abstract: SELDI-TOF MS analysis of cyst fluids identified 95 peaks that discriminate malignant, borderline, and benign ovarian tumors. Three prominent peaks, which correspond to calgranulin A (m/z 10847) and two isoforms of calgranulin B (m/z 12717 and 13294), have higher concentrations in borderline and malignant cyst fluids. Together, calgranulin A and B distinguish borderline and malignant tumors from benign tumors with 28.6% and 63.6% sensitivity for early stage disease, respectively, at 95% specificity and with 74.8% accuracy. Ovarian cyst fluids are useful for discovering discriminatory biomarkers, such as calgranulin, which may have utility for detecting, diagnosing, and biochemically classifying ovarian tumors.
authors.affiliation: Mylan Pharmaceuticals Inc., Morgantown, West Virginia, USA.
authors.forename: Hollie S
authors.initials: HS
authors.lastname: Skaggs
authors.forename: Brook A
authors.initials: BA
authors.lastname: Saunders
authors.forename: Rachel W
authors.initials: RW
authors.lastname: Miller
authors.forename: Scott T
authors.initials: ST
authors.lastname: Goodrich
authors.forename: Monica S
authors.initials: MS
authors.lastname: King
authors.forename: Kimberly D
authors.initials: KD
authors.lastname: Kimbler
authors.forename: Adam J
authors.initials: AJ
authors.lastname: Branscum
authors.forename: Eric T
authors.initials: ET
authors.lastname: Fung
authors.forename: Paul D
authors.initials: PD
authors.lastname: DePriest
authors.forename: John R
authors.initials: JR
authors.lastname: van Nagell
authors.forename: Frederick R
authors.initials: FR
authors.lastname: Ueland
authors.forename: Andre T
authors.initials: AT
authors.lastname: Baron
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000032:analysis*; D015153:Blotting, Western; D040501:Calgranulin A / Q000032:analysis* / Q000096:biosynthesis; D040502:Calgranulin B / Q000032:analysis* / Q000096:biosynthesis; D020420:Cyst Fluid / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D008875:Middle Aged; D010048:Ovarian Cysts / Q000737:chemistry*; D010051:Ovarian Neoplasms / Q000175:diagnosis* / Q000378:metabolism; D020033:Protein Isoforms / Q000032:analysis / Q000096:biosynthesis; D012680:Sensitivity and Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D014408:Biomarkers, Tumor; D040501:Calgranulin A; D040502:Calgranulin B; D020033:Protein Isoforms