title: [Optimize extraction process of polysaccharide in Trillium tschonoskii].
abstract: OBJECTIVE
  To optimize the extraction process of polysaccharide in Trillium tschonoskii.
  
  
  METHODS
  The influence of temperature, time, solid-liquid ratio and extraction times on extraction yield of the polysaccharide in fleshy roots of Trillium tschonokii were discussed with orthogonal test method.
  
  
  RESULTS
  The impact sequence of the factors on the extraction rate of polysaccharide in Trillium tschonoskii was as follows: extraction times > time > solid-liquid ratio > temperature; The optimal extraction condition was extraction temperature of 80 degrees C, extraction time of 4.5h, solid-liquid ratio of 1:40 and extracted three times.
  
  
  CONCLUSIONS
  Under these optimal conditions, the extracting rates of polysaccharide in Trillium tschonoskii is 6.75%. The content of polysaccharide is high which can be exploited and utilized as another new ingredient.
authors.affiliation: Key Laboratory of Biological Resources Protection and Utilization of Hubei Province, Enshi 445000, China. 876478477@qq.com
authors.forename: Jing-Jing
authors.initials: JJ
authors.lastname: Xu
authors.forename: Xun-Ru
authors.initials: XR
authors.lastname: Ai
authors.forename: Qin
authors.initials: Q
authors.lastname: Li
authors.forename: Hao
authors.initials: H
authors.lastname: Xiao
authors.forename: Xiao-Jiang
authors.initials: XJ
authors.lastname: Zheng
mesh_terms: D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification*; D011134:Polysaccharides / Q000032:analysis / Q000302:isolation & purification*; D011786:Quality Control; D015203:Reproducibility of Results; D027343:Rhizome / Q000737:chemistry; D013678:Technology, Pharmaceutical / Q000379:methods*; D013696:Temperature; D013997:Time Factors; D031446:Trillium / Q000737:chemistry*
chemical_list: D004365:Drugs, Chinese Herbal; D011134:Polysaccharides