title: Green targeting predictor and ambiguous targeting predictor 2: the pitfalls of plant protein targeting prediction and of transient protein expression in heterologous systems.
abstract: The challenges of plant protein targeting prediction are the existence of dual subcellular targets and the bias of experimentally confirmed data towards few and mostly nonplant model species. To assess whether training with proteins from evolutionarily distant species has a negative impact on prediction accuracy, we developed the Green Targeting Predictor tool, which was trained with a species-specific data set for Physcomitrella patens. Its performance was compared with that of the same tool trained with a mixed data set. In addition, we updated the Ambiguous Targeting Predictor. We found that predictions deviated from in vivo observations predominantly for proteins diverging within the green lineage, as well as for dual targeted proteins. To evaluate the usefulness of heterologous expression systems, selected proteins were subjected to localization studies in P. patens, Arabidopsis thaliana and Nicotiana tabacum. Four out of six proteins that show dual targeting in the original plant system were located only in a single compartment in one or both heterologous systems. We conclude that targeting signals of divergent plant species exhibit differences, calling for custom in silico and in vivo approaches when aiming to unravel the actual distribution patterns of proteins within a plant cell.
authors.affiliation: Department of Arctic and Marine Biology, University of Tromsø, Dramsvegen 201, N-9037, Tromsø, Norway.
authors.forename: Janina
authors.initials: J
authors.lastname: Fuss
authors.forename: Oliver
authors.initials: O
authors.lastname: Liegmann
authors.forename: Kirsten
authors.initials: K
authors.lastname: Krause
authors.forename: Stefan A
authors.initials: SA
authors.lastname: Rensing
mesh_terms: D017360:Arabidopsis / Q000378:metabolism; D019068:Bryopsida / Q000378:metabolism*; D003198:Computer Simulation; D030562:Databases, Protein; D015870:Gene Expression; D010940:Plant Proteins / Q000378:metabolism*; D010944:Plants / Q000378:metabolism*; D021382:Protein Sorting Signals; D021381:Protein Transport; D015203:Reproducibility of Results; D012984:Software; D013045:Species Specificity; D014026:Nicotiana / Q000378:metabolism
chemical_list: D010940:Plant Proteins; D021382:Protein Sorting Signals
keywords: chloroplasts; dual targeting; evolution; mitochondria; protein import; targeting prediction