title: Thymic stromal lymphopoietin induces migration in human airway smooth muscle cells.
abstract: Airway remodeling due to increased airway smooth muscle (ASM) mass, likely due to enhanced migration and proliferation, has been shown to be highly associated with decline in lung function in asthma. Thymic stromal lymphopoietin (TSLP) is an IL-7-like, pro-allergic cytokine that has been shown to be necessary and sufficient for the development of allergic asthma. Human ASM (HASM) cells express TSLP receptor (TSLPR), the activation of which leads to enhanced release of proinflammatory mediators such as IL-6, CCL11/eotaxin-1, and CXCL8/IL-8. We show here that TSLP induces HASM cell migration through STAT3 activation since lentiviral-shRNA inhibition of STAT3 abrogated the TSLP-induced cell migration. Moreover, TSLP induced multiple cytoskeleton changes in HASM cells such as actin polymerization, cell polarization, and activation of small GTPase Rac1. Collectively, our data suggest a pro-migratory function of TSLP in ASM remodeling and provides better rationale for targeting TSLP/TSLPR pathway for therapeutic approaches in allergic asthma.
authors.affiliation: Department of Immunology, Faculty of Medicine, University of Manitoba, Winnipeg, MB, Canada.
authors.forename: Naresh Singh
authors.initials: NS
authors.lastname: Redhu
authors.forename: Lianyu
authors.initials: L
authors.lastname: Shan
authors.forename: Hesam
authors.initials: H
authors.lastname: Movassagh
authors.forename: Abdelilah S
authors.initials: AS
authors.lastname: Gounni
mesh_terms: D056151:Airway Remodeling; D001249:Asthma / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D002465:Cell Movement / Q000187:drug effects*; D016207:Cytokines / Q000494:pharmacology*; D003599:Cytoskeleton / Q000378:metabolism / Q000473:pathology; D006801:Humans; D032389:Myocytes, Smooth Muscle / Q000187:drug effects* / Q000378:metabolism*; D050796:STAT3 Transcription Factor / Q000378:metabolism; D020830:rac1 GTP-Binding Protein / Q000378:metabolism; D000094632:Thymic Stromal Lymphopoietin
chemical_list: D016207:Cytokines; D050796:STAT3 Transcription Factor; D020830:rac1 GTP-Binding Protein; D000094632:Thymic Stromal Lymphopoietin
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