title: Preliminary X-ray crystallographic studies of the short form of the human TRAF4 TRAF domain.
abstract: TNF (tumour necrosis factor) receptor-associated factor 4 (TRAF4) is a unique TRAF protein that participates in morphogenetic and developmental function and cell migration. TRAF-family proteins contain a TRAF domain for target interaction. In this study, the short form of the human TRAF4 TRAF domain, corresponding to amino acids 290-462, was overexpressed in Escherichia coli using engineered C-terminal His tags. The short form of the TRAF4 TRAF domain was purified to homogeneity and crystallized at 293 K. Finally, X-ray diffraction data were collected to a resolution of 4.2 Å from a crystal belonging to the hexagonal space group P3₂, with unit-cell parameters a = b = 147.17, c = 202.69 Å.
authors.affiliation: School of Biotechnology and Graduate School of Biochemistry, Yeungnam University, Gyeongsan, Republic of Korea.
authors.forename: Jong Hwan
authors.initials: JH
authors.lastname: Yoon
authors.forename: Hyun Ho
authors.initials: HH
authors.lastname: Park
mesh_terms: D018360:Crystallography, X-Ray; D006801:Humans; D010446:Peptide Fragments / Q000737:chemistry / Q000235:genetics; D017434:Protein Structure, Tertiary / Q000235:genetics; D053449:TNF Receptor-Associated Factor 4 / Q000737:chemistry* / Q000235:genetics*
chemical_list: D010446:Peptide Fragments; D053449:TNF Receptor-Associated Factor 4; C506367:TRAF4 protein, human
keywords: TRAF4 TRAF domain
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