title: Review on Cardamine diphylla (Michx.) A. wood (Brassicaceae): ethnobotany and glucosinolate chemistry.
abstract: BACKGROUND
  Cardamine diphylla (Michx.) A. Wood, commonly called toothwort, is a spring perennial herb belonging to the Brassicaceae family. This endemic plant of Eastern North America has been widely used by multiple American First Nations (i.e. indigenous people of North America) for food and medicine for centuries.
  
  
  METHODS
  The aim of the review is to describe the botany, ethnopharmacology, phytochemistry, and bioactivity of Cardamine diphylla. The review covers literature on Cardamine diphylla, and the alternative name Dentaria diphylla, from English and French language sources.
  
  
  RESULTS
  Multiple traditional uses of Cardamine diphylla by American First Nations are well documented. Initial health studies showed that the tested concentrations of the extract were not toxic against brine shrimp larvae and the same extract had a weak free-radical scavenging activity. However, bioactive compounds in the form of aliphatic and indole glucosinolates and some indole alkaloids have been isolated from this plant. Ecological research regarding Cardamine diphylla-insect interactions (such as feeding and oviposition) is also available in the literature.
  
  
  CONCLUSIONS
  The wide range of traditional uses by multiple American First Nations suggests that the antibacterial, antiviral, immunostimulant, analgesic, antipyretic, and anti-inflammatory activities of this plant should be explored in in vitro and in vivo tests. Traditional modes of preparation of the plant suggest that some of the medicinal properties could certainly be attributed to glucosinolate degradation products (i.e. isothiocyanates), but a clear assignment of active molecules and mechanisms of action remain to be elucidated. The presence of glucosinolates indicates that the plant could be probed for cancer chemopreventive properties. Overall, the review shows that more investigation is necessary to determine the possible benefits of Cardamine diphylla extracts to pharmaceutical companies as a nutraceutic specialty phytotherapeutic agent against respiratory (cold and sore throat) or gastrointestinal problems.
authors.affiliation: Department of Chemistry & Biochemistry, Biomolecular Sciences Programme, Laurentian University, Sudbury, ON, Canada. smontaut@laurentian.ca
authors.forename: Sabine
authors.initials: S
authors.lastname: Montaut
authors.forename: René S
authors.initials: RS
authors.lastname: Bleeker
mesh_terms: D000818:Animals; D031217:Cardamine / Q000033:anatomy & histology / Q000737:chemistry; D019448:Ethnobotany; D005961:Glucosinolates / Q000302:isolation & purification; D006801:Humans; D007198:Indians, North American; D008519:Medicine, Traditional; D009656:North America; D010936:Plant Extracts / Q000494:pharmacology
chemical_list: D005961:Glucosinolates; D010936:Plant Extracts
keywords: 2,2′-Azino-bis(3-ethylbenzthiazoline-6-sulfonic acid); ABTS(+•); Alkaloids; Brassicaceae; CYP; Cardamine diphylla; Cytochrome P450; DPPH(•); Diphenylpicrylhydrazyl radical; Free-radical scavenging activity; GC; GL; GLs; Gas chromatography; Glucosinolate; Glucosinolates; HPLC; Half maximal scavenging activity; High performance liquid chromatography; ITC; ITCs; Isothiocyanate; Isothiocyanates; LC–MS; Liquid chromatography–mass spectrometry; SC(50)