title: The urokinase receptor homolog Haldisin is a novel differentiation marker of stratum granulosum in squamous epithelia.
abstract: Several members of the Ly-6/uPAR (LU)-protein domain family are differentially expressed in human squamous epithelia. In some cases, they even play important roles in maintaining skin homeostasis, as exemplified by the secreted single domain member, SLURP-1, the deficiency of which is associated with the development of palmoplantar hyperkeratosis in the congenital skin disorder Mal de Meleda. In the present study, we have characterized a new member of the LU-protein domain family, which we find to be predominantly expressed in the stratum granulosum of human skin, thus resembling the expression of SLURP-1. In accordance with its expression pattern, we denote this protein product, which is encoded by the LYPD5 gene, as Haldisin (human antigen with LU-domains expressed in skin). Two of the five human glycolipid-anchored membrane proteins with multiple LU-domains characterized so far are predominantly confined to squamous epithelia (i.e., C4.4A), to stratum spinosum, and Haldisin to stratum granulosum under normal homeostatic conditions. Whether Haldisin is a prognostic biomarker for certain epithelial malignancies, like C4.4A and SLURP-1, remains to be explored.
authors.affiliation: The Finsen Laboratory, Rigshospitalet & Biotech Research and Innovation Centre, Copenhagen Biocenter, Copenhagen, Denmark (HG,MCK,EPH,WAA,MP).
authors.forename: Henrik
authors.initials: H
authors.lastname: Gårdsvoll
authors.forename: Mette C
authors.initials: MC
authors.lastname: Kriegbaum
authors.forename: Emil P
authors.initials: EP
authors.lastname: Hertz
authors.forename: Warner
authors.initials: W
authors.lastname: Alpízar-Alpízar
authors.forename: Michael
authors.initials: M
authors.lastname: Ploug
mesh_terms: D000818:Animals; D003001:Cloning, Molecular; D004330:Drosophila; D004817:Epidermis / Q000378:metabolism* / Q000473:pathology*; D005455:Fluorescent Antibody Technique; D015870:Gene Expression; D015972:Gene Expression Regulation, Neoplastic; D057809:HEK293 Cells; D006801:Humans; D007150:Immunohistochemistry; D051379:Mice; D018307:Neoplasms, Squamous Cell / Q000235:genetics / Q000473:pathology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D055293:Receptors, Urokinase Plasminogen Activator / Q000032:analysis* / Q000235:genetics; D011994:Recombinant Proteins / Q000032:analysis / Q000235:genetics
chemical_list: C585232:LYPD5 protein, human; D055293:Receptors, Urokinase Plasminogen Activator; D011994:Recombinant Proteins
keywords: C4.4A; Haldisin; LU; LYPD5; Ly-6/uPAR protein domain family; PRO4356; differentiation marker; squamous epithelium; stratum granulosum; uPAR
references.citation: Protein Expr Purif. 2007 Apr;52(2):384-94
references.pmid: 17215141
references.citation: Biol Reprod. 2001 Mar;64(3):935-45
references.pmid: 11207211
references.citation: J Biol Chem. 2008 Dec 19;283(51):35976-82
references.pmid: 18854317
references.citation: J Leukoc Biol. 2013 Oct;94(4):585-94
references.pmid: 23543767
references.citation: EMBO J. 2005 May 4;24(9):1655-63
references.pmid: 15861141
references.citation: J Thorac Oncol. 2013 Feb;8(2):152-60
references.pmid: 23287851
references.citation: Cell Metab. 2010 Jul 7;12(1):42-52
references.pmid: 20620994
references.citation: Science. 2006 Feb 3;311(5761):656-9
references.pmid: 16456079
references.citation: Curr Drug Targets. 2011 Nov;12(12):1711-28
references.pmid: 21707479
references.citation: Toxicon. 2010 Nov;56(6):855-67
references.pmid: 20670641
references.citation: FEBS Lett. 2012 Jul 4;586(14):1860-70
references.pmid: 22651914
references.citation: Cell Mol Life Sci. 2008 Nov;65(21):3481-93
references.pmid: 18821057
references.citation: Genes Dev. 2013 Mar 1;27(5):459-84
references.pmid: 23475957
references.citation: Development. 2010 Jul;137(14):2427-37
references.pmid: 20570942
references.citation: FEBS Lett. 1994 Aug 1;349(2):163-8
references.pmid: 8050560
references.citation: Int J Cancer. 2012 Jun 1;130(11):2734-9
references.pmid: 21792890
references.citation: Lung Cancer. 2007 Nov;58(2):260-6
references.pmid: 17706320
references.citation: Protein Expr Purif. 2010 Jul;72(1):87-94
references.pmid: 20214994
references.citation: J Biol Chem. 2011 Dec 16;286(50):43515-26
references.pmid: 22025616
references.citation: J Histochem Cytochem. 2011 Feb;59(2):188-201
references.pmid: 21339181
references.citation: Cancer Sci. 2010 Oct;101(10):2269-77
references.pmid: 20825414
references.citation: Front Biosci. 2008 May 01;13:5441-61
references.pmid: 18508598
references.citation: J Biol Chem. 2011 Sep 23;286(38):33544-56
references.pmid: 21799009
references.citation: Br J Cancer. 2007 Oct 22;97(8):1146-56
references.pmid: 17912244
references.citation: Biochem J. 2004 Jun 15;380(Pt 3):845-57
references.pmid: 15012588
references.citation: J Biol Chem. 2012 Oct 5;287(41):34304-15
references.pmid: 22896701
references.citation: Proc Natl Acad Sci U S A. 2013 May 14;110(20):8111-6
references.pmid: 23633567
references.citation: J Neurosci. 2011 May 25;31(21):7876-85
references.pmid: 21613501
references.citation: J Invest Dermatol. 2007 Feb;127(2):301-8
references.pmid: 17008884
references.citation: Protein Expr Purif. 2004 Apr;34(2):284-95
references.pmid: 15003263
references.citation: Blood. 2013 Jan 3;121(1):129-35
references.pmid: 23149847
references.citation: Theranostics. 2013 Jun 24;3(7):467-76
references.pmid: 23843894