title: Hepatoprotective effect of commercial herbal extracts on carbon tetrachloride-induced liver damage in Wistar rats.
abstract: BACKGROUND
  Various hepatoprotective herbal products from plants are available in Mexico, where up to 85% of patients with liver disease use some form of complementary and alternative medicine. However, only few studies have reported on the biological evaluation of these products.
  
  
  OBJECTIVE
  Using a model of carbon tetrachloride (CCl4)-induced hepatotoxicity in rats, we evaluated the effects of commercial herbal extracts used most commonly in the metropolitan area of Monterrey, Mexico.
  
  
  METHODS
  The commercial products were identified through surveys in public areas. The effect of these products given with or without CCl4 in rats was evaluated by measuring the serum concentrations of aspartate amino transferase (AST) and alanine amino transferase (ALT), and histopathological analysis. Legalon(®) was used as the standard drug.
  
  
  RESULTS
  The most commonly used herbal products were Hepatisan(®) capsules, Boldo capsules, Hepavida(®) capsules, Boldo infusion, and milk thistle herbal supplement (80% silymarin). None of the products tested was hepatotoxic according to transaminase and histological analyses. AST and ALT activities were significantly lower in the Hepavida+CCl4-treated group as compared with the CCl4-only group. AST and ALT activities in the silymarin, Hepatisan, and Boldo tea groups were similar to those in the CCl4 group. The CCl4 group displayed submassive confluent necrosis and mixed inflammatory infiltration. Both the Hepatisan+CCl4 and Boldo tea+CCl4 groups exhibited ballooning degeneration, inflammatory infiltration, and lytic necrosis. The silymarin+CCl4 group exhibited microvesicular steatosis. The Hepavida+CCl4- and Legalon+CCL4-treated groups had lower percentages of necrotic cells as compared with the CCl4-treated group; this treatment was hepatoprotective against necrosis.
  
  
  CONCLUSIONS
  Only Hepavida had a hepatoprotective effect.
authors.affiliation: Liver Unit, Gastroenterology Service from Department of Internal Medicine, Universidad Autónoma de Nuevo León, Av. Gonzalitos S/N Col. Mitras Centro C.P., Monterrey, Nuevo León, México.
authors.forename: Paula
authors.initials: P
authors.lastname: Cordero-Pérez
authors.forename: Liliana
authors.initials: L
authors.lastname: Torres-González
authors.forename: Marcelino
authors.initials: M
authors.lastname: Aguirre-Garza
authors.forename: Carlos
authors.initials: C
authors.lastname: Camara-Lemarroy
authors.forename: Francisco
authors.initials: F
authors.lastname: Guzmán-de la Garza
authors.forename: Gabriela
authors.initials: G
authors.lastname: Alarcón-Galván
authors.forename: Homero
authors.initials: H
authors.lastname: Zapata-Chavira
authors.forename: Ma
authors.initials: M
authors.lastname: de Jesús Sotelo-Gallegos
authors.forename: Cipactli
authors.initials: C
authors.lastname: Nadjedja Torres-Esquivel
authors.forename: Ethel
authors.initials: E
authors.lastname: Sánchez-Fresno
authors.forename: Daniel
authors.initials: D
authors.lastname: Cantú-Sepúlveda
authors.forename: Gerardo
authors.initials: G
authors.lastname: González-Saldivar
authors.forename: Judith
authors.initials: J
authors.lastname: Bernal-Ramirez
authors.forename: Linda
authors.initials: L
authors.lastname: E Muñoz-Espinosa
keywords: Alanine transferase; aspartate transferase; hepatoprotection; liver injury; natural products
references.citation: Gen Pharmacol. 1999 Jun;32(6):661-7
references.pmid: 10401991
references.citation: Toxicol Lett. 2009 Sep 10;189(2):159-65
references.pmid: 19481141
references.citation: Altern Med Rev. 1999 Jun;4(3):178-88
references.pmid: 10383482
references.citation: Pharmacol Ther. 1989;43(1):139-54
references.pmid: 2675128
references.citation: Altern Med Rev. 1998 Dec;3(6):410-21
references.pmid: 9855566
references.citation: Baillieres Clin Gastroenterol. 1988 Apr;2(2):481-500
references.pmid: 3044472
references.citation: J Viral Hepat. 2005 Nov;12(6):559-67
references.pmid: 16255756
references.citation: Phytomedicine. 2007 Feb;14(2-3):129-35
references.pmid: 16638633
references.citation: N Engl J Med. 2002 Dec 19;347(25):2046-56
references.pmid: 12490687
references.citation: J Clin Gastroenterol. 2005 Jul;39(6):520-8
references.pmid: 15942440
references.citation: J Pharmacol Toxicol Methods. 2002 Jul-Aug;48(1):41-4
references.pmid: 12750040
references.citation: Gastroenterology. 2008 May;134(6):1682-98
references.pmid: 18471547
references.citation: ScientificWorldJournal. 2011;11:1886-92
references.pmid: 22125445
references.citation: Salud Publica Mex. 1999 May-Jun;41(3):216-20
references.pmid: 10420791
references.citation: Semin Liver Dis. 2009 May;29(2):211-21
references.pmid: 19387920
references.citation: Semin Liver Dis. 2008 Nov;28(4):396-406
references.pmid: 18956296
references.citation: Liver Int. 2009 Jan;29 Suppl 1:9-14
references.pmid: 19207960
references.citation: Am J Gastroenterol. 2002 Sep;97(9):2391-7
references.pmid: 12358262
references.citation: Life Sci. 2003 Apr 4;72(20):2273-83
references.pmid: 12628447
references.citation: Liver Int. 2007 Sep;27(7):879-90
references.pmid: 17696925
references.citation: Semin Liver Dis. 2002;22(2):195-206
references.pmid: 12016550
references.citation: Am J Gastroenterol. 1998 Feb;93(2):139-43
references.pmid: 9468229
references.citation: Ann Intern Med. 1992 Jul 15;117(2):129-32
references.pmid: 1605427
references.citation: J Ethnopharmacol. 2007 May 30;112(1):145-51
references.pmid: 17391882
references.citation: Hepatology. 2009 May;49(5 Suppl):S174-84
references.pmid: 19399794
references.citation: J Hepatol. 1997;26 Suppl 1:47-51
references.pmid: 9138129
references.citation: Planta Med. 1991 Apr;57(2):110-5
references.pmid: 1891491
references.citation: Liver Int. 2007 Feb;27(1):17-25
references.pmid: 17241377
references.citation: J Viral Hepat. 2008 Jul;15(7):508-14
references.pmid: 18397225
references.citation: Ann Hepatol. 2009;8 Suppl 1:S51-9
references.pmid: 19381125
references.citation: World J Gastroenterol. 2009 Aug 21;15(31):3901-7
references.pmid: 19701970
references.citation: Phytother Res. 2009 Jul;23(7):1024-7
references.pmid: 19145575
references.citation: Mutat Res. 2010 Jan;695(1-2):55-60
references.pmid: 19945544
references.citation: Ann Intern Med. 2001 Aug 21;135(4):262-8
references.pmid: 11511141
references.citation: BMJ. 1989 Nov 4;299(6708):1156-7
references.pmid: 2513032