title: Cell-bound exopolysaccharides of Lactobacillus brevis KB290: protective role and monosaccharide composition.
abstract: We examined the survivability of Lactobacillus brevis KB290 and derivative strain KB392 in artificial digestive juices and bile salts. The strains have similar membrane fatty acids but different amounts of cell-bound exopolysaccharides (EPS). In artificial digestive juices, KB290 showed significantly higher survivability than KB392, and homogenization, which reduced the amount of EPS in KB290 but not in KB392, reduced the survivability only of KB290. In bile salts, KB290 showed significantly higher survivability than KB392, and cell-bound EPS extraction with EDTA reduced the survivability of only KB290. Transmission electron microscopy showed there to be a greater concentration of cell-bound EPS in KB290 than in either KB392 or EDTA-treated or homogenized KB290. We conclude that KB290's cell-bound EPS (which high performance liquid chromatography showed to be made up of glucose and N-acetylglucosamine) played an important role in bile salt tolerance.
authors.affiliation: Research and Development Division, Kagome Co., Ltd., 17 Nishitomiyama, Nasushiobara 329-2762, Japan. Shigenori_Suzuki@kagome.co.jp
authors.forename: Shigenori
authors.initials: S
authors.lastname: Suzuki
authors.forename: Takafumi
authors.initials: T
authors.lastname: Yakabe
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Suganuma
authors.forename: Masanori
authors.initials: M
authors.lastname: Fukao
authors.forename: Tadao
authors.initials: T
authors.lastname: Saito
authors.forename: Nobuhiro
authors.initials: N
authors.lastname: Yajima
mesh_terms: D001647:Bile Acids and Salts / Q000494:pharmacology; D002614:Chelating Agents / Q000494:pharmacology; D004492:Edetic Acid / Q000494:pharmacology; D005750:Gastric Juice / Q000382:microbiology; D052196:Levilactobacillus brevis / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D008563:Membrane Lipids / Q000737:chemistry*; D050296:Microbial Viability / Q000187:drug effects; D009005:Monosaccharides / Q000032:analysis*; D011135:Polysaccharides, Bacterial / Q000737:chemistry* / Q000378:metabolism*
chemical_list: D001647:Bile Acids and Salts; D002614:Chelating Agents; D008563:Membrane Lipids; D009005:Monosaccharides; D011135:Polysaccharides, Bacterial; D004492:Edetic Acid