title: An interleukin-33/ST2 signaling deficiency reduces overt pain-like behaviors in mice.
abstract: Interleukin (IL)-33, the most recent member of the IL family of cytokines, signals through the ST2 receptor. IL-33/ST2 signaling mediates antigen challenge-induced mechanical hyperalgesia in the joints and cutaneous tissues of immunized mice. The present study asked whether IL-33/ST2 signaling is relevant to overt pain-like behaviors in mice. Acetic acid and phenyl-p-benzoquinone induced significant writhing responses in wild-type (WT) mice; this overt nociceptive behavior was reduced in ST2-deficient mice. In an antigen-challenge model, ST2-deficient immunized mice had reduced induced flinch and licking overt pain-like behaviors. In the formalin test, ST2-deficient mice also presented reduced flinch and licking responses, compared with WT mice. Naive WT and ST2-deficient mice presented similar responses in the rota-rod, hot plate, and electronic von Frey tests, indicating no impairment of motor function or alteration in basal nociceptive responses. The results demonstrate that IL-33/ST2 signaling is important in the development of overt pain-like behaviors.
authors.affiliation: Universidade de São Paulo, Departamento de Farmacologia, Faculdade de Medicina de Ribeirão Preto, Ribeirão PretoSP, Brasil.
authors.forename: D A C
authors.initials: DA
authors.lastname: Magro
authors.forename: M S N
authors.initials: MS
authors.lastname: Hohmann
authors.forename: S S
authors.initials: SS
authors.lastname: Mizokami
authors.forename: T M
authors.initials: TM
authors.lastname: Cunha
authors.forename: J C
authors.initials: JC
authors.lastname: Alves-Filho
authors.forename: R
authors.initials: R
authors.lastname: Casagrande
authors.forename: S H
authors.initials: SH
authors.lastname: Ferreira
authors.forename: F Y
authors.initials: FY
authors.lastname: Liew
authors.forename: F Q
authors.initials: FQ
authors.lastname: Cunha
authors.forename: W A
authors.initials: WA
authors.lastname: Verri
mesh_terms: D019342:Acetic Acid; D000818:Animals; D016227:Benzoquinones; D006720:Homozygote; D006358:Hot Temperature; D006930:Hyperalgesia / Q000378:metabolism*; D000072179:Interleukin-1 Receptor-Like 1 Protein; D000067596:Interleukin-33; D007378:Interleukins / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D009043:Motor Activity / Q000502:physiology; D059225:Nociception / Q000502:physiology; D059226:Nociceptive Pain / Q000139:chemically induced / Q000503:physiopathology*; D010047:Ovalbumin / Q000276:immunology; D010147:Pain Measurement / Q000379:methods*; D018123:Receptors, Interleukin / Q000172:deficiency*; D045442:Rotarod Performance Test; D015398:Signal Transduction
chemical_list: D016227:Benzoquinones; C486189:Il1rl1 protein, mouse; C506535:Il33 protein, mouse; D000072179:Interleukin-1 Receptor-Like 1 Protein; D000067596:Interleukin-33; D007378:Interleukins; D018123:Receptors, Interleukin; C029322:phenylbenzoquinone; D010047:Ovalbumin; D019342:Acetic Acid
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