title: Motility-, autocorrelation-, and polarization-sensitive optical coherence tomography discriminates cells and gold nanorods within 3D tissue cultures.
abstract: We propose a method for differentiating classes of light scatterers based upon their temporal and polarization properties computed from time series of polarization-sensitive optical coherence tomography (PS-OCT) images. The amplitude (motility) and time scale (autocorrelation decay time) of the speckle fluctuations are combined with the cross-polarization pixel-wise to render Motility-, autocorrelation-, and polarization-sensitive (MAPS) OCT contrast images. This combination of metrics provides high specificity for discriminating diffusive gold nanorods and mammary epithelial cell spheroids within 3D tissue culture, based on their unique MAPS signature. This has implications toward highly specific contrast in molecular (nanoparticle-based) and functional (cellular activity) imaging using standard PS-OCT hardware.
authors.affiliation: Department of Physics and Astronomy, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599-3255, USA. aold@physics.unc.edu
authors.forename: Amy L
authors.initials: AL
authors.lastname: Oldenburg
authors.forename: Raghav K
authors.initials: RK
authors.lastname: Chhetri
authors.forename: Jason M
authors.initials: JM
authors.lastname: Cooper
authors.forename: Wei-Chen
authors.initials: WC
authors.lastname: Wu
authors.forename: Melissa A
authors.initials: MA
authors.lastname: Troester
authors.forename: Joseph B
authors.initials: JB
authors.lastname: Tracy
mesh_terms: D006046:Gold / Q000737:chemistry*; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D008027:Light; D042361:Mammary Glands, Human / Q000166:cytology*; D043942:Nanotubes; D012542:Scattering, Radiation; D046509:Tissue Culture Techniques; D041623:Tomography, Optical Coherence / Q000379:methods*
chemical_list: D006046:Gold
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