title: Double-membraned liposomes sculpted by poliovirus 3AB protein.
abstract: Infection with many positive-strand RNA viruses dramatically remodels cellular membranes, resulting in the accumulation of double-membraned vesicles that resemble cellular autophagosomes. In this study, a single protein encoded by poliovirus, 3AB, is shown to be sufficient to induce the formation of double-membraned liposomes via the invagination of single-membraned liposomes. Poliovirus 3AB is a 109-amino acid protein with a natively unstructured N-terminal domain. HeLa cells transduced with 3AB protein displayed intracellular membrane disruption; specifically, the formation of cytoplasmic invaginations. The ability of a single viral protein to produce structures of similar topology to cellular autophagosomes should facilitate the understanding of both cellular and viral mechanisms for membrane remodeling.
authors.affiliation: Department of Physiology and Biophysics, Boston University School of Medicine, Boston, Massachusetts 02118.
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, California 94301.
authors.forename: Jennifer B
authors.initials: JB
authors.lastname: Ptacek
authors.affiliation: Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, California 94301. Electronic address: karlak@stanford.edu.
authors.forename: Karla
authors.initials: K
authors.lastname: Kirkegaard
authors.affiliation: Department of Physiology and Biophysics, Boston University School of Medicine, Boston, Massachusetts 02118. Electronic address: bullitt@bu.edu.
authors.forename: Esther
authors.initials: E
authors.lastname: Bullitt
mesh_terms: D002462:Cell Membrane / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D008081:Liposomes / Q000737:chemistry* / Q000378:metabolism; D008565:Membrane Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D017955:Poliovirus; D017434:Protein Structure, Tertiary; D017361:Viral Nonstructural Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism
chemical_list: C520978:3AB protein, poliovirus; D008081:Liposomes; D008565:Membrane Proteins; D017361:Viral Nonstructural Proteins
keywords: Lipoprotein; Liposomes; Membrane Bilayer; Membrane Biophysics; Membrane Reconstitution; Positive-strand RNA Viruses; Viral Protein; Viral Replication
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