title: Silencing Agrobacterium oncogenes in transgenic grapevine results in strain-specific crown gall resistance.
abstract: CONCLUSIONS
  Grapevine rootstock transformed with an Agrobacterium oncogene-silencing transgene was resistant to certain Agrobacterium strains but sensitive to others. Thus, genetic diversity of Agrobacterium oncogenes may limit engineering crown gall resistance.
  Crown gall disease of grapevine induced by Agrobacterium vitis or Agrobacterium tumefaciens causes serious economic losses in viticulture. To establish crown gall-resistant lines, somatic proembryos of Vitis berlandieri × V. rupestris cv. 'Richter 110' rootstock were transformed with an oncogene-silencing transgene based on iaaM and ipt oncogene sequences from octopine-type, tumor-inducing (Ti) plasmid pTiA6. Twenty-one transgenic lines were selected, and their transgenic nature was confirmed by polymerase chain reaction (PCR). These lines were inoculated with two A. tumefaciens and three A. vitis strains. Eight lines showed resistance to octopine-type A. tumefaciens A348. Resistance correlated with the expression of the silencing genes. However, oncogene silencing was mostly sequence specific because these lines did not abolish tumorigenesis by A. vitis strains or nopaline-type A. tumefaciens C58.
authors.affiliation: Department of Genetics and Molecular Biology, Faculty of Sciences, University of Pécs, Ifjúság útja 6, Pécs, 7624, Hungary.
authors.forename: A
authors.initials: A
authors.lastname: Galambos
authors.forename: A
authors.initials: A
authors.lastname: Zok
authors.forename: A
authors.initials: A
authors.lastname: Kuczmog
authors.forename: R
authors.initials: R
authors.lastname: Oláh
authors.forename: P
authors.initials: P
authors.lastname: Putnoky
authors.forename: W
authors.initials: W
authors.lastname: Ream
authors.forename: E
authors.initials: E
authors.lastname: Szegedi
mesh_terms: D060054:Agrobacterium / Q000235:genetics* / Q000472:pathogenicity; D003433:Crosses, Genetic; D004269:DNA, Bacterial / Q000235:genetics; D060467:Disease Resistance; D020868:Gene Silencing; D005798:Genes, Bacterial; D009857:Oncogenes / Q000235:genetics*; D010941:Plant Tumors / Q000382:microbiology*; D030821:Plants, Genetically Modified; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic / Q000235:genetics; D018749:RNA, Plant / Q000235:genetics / Q000378:metabolism; D013045:Species Specificity; D014170:Transformation, Genetic; D019076:Transgenes / Q000235:genetics; D014774:Virulence; D027843:Vitis / Q000235:genetics* / Q000382:microbiology*
chemical_list: D004269:DNA, Bacterial; D018749:RNA, Plant; C036483:T-DNA
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