title: Evaluation of antibiotic releasing porous polymethylmethacrylate space maintainers in an infected composite tissue defect model.
abstract: This study evaluated the in vitro and in vivo performance of antibiotic-releasing porous polymethylmethacrylate (PMMA)-based space maintainers comprising a gelatin hydrogel porogen and a poly(dl-lactic-co-glycolic acid) (PLGA) particulate carrier for antibiotic delivery. Colistin was released in vitro from either gelatin or PLGA microparticle loaded PMMA constructs, with gelatin-loaded constructs releasing colistin over approximately 7 days and PLGA microparticle-loaded constructs releasing colistin for up to 8 weeks. Three formulations with either burst release or extended release at different doses were tested in a rabbit mandibular defect inoculated with Acinetobacter baumannii (2×10(7) colony forming units ml(-1)). In addition, one material control that released antibiotic but was not inoculated with A. baumannii was tested. A. baumannii was not detectable in any animal after 12 weeks on culture of the defect, saliva, or blood. Defects with high dose extended release implants had greater soft tissue healing compared with defects with burst release implants, with 8 of 10 animals showing healed mucosae compared with 2 of 10 respectively. Extended release of locally delivered colistin via a PLGA microparticle carrier improved soft tissue healing compared with implants with burst release of colistin from a gelatin carrier.
authors.affiliation: Department of Bioengineering, Rice University, MS-142, 6100 Main Street, Houston, TX 77005, USA.
authors.forename: Patrick P
authors.initials: PP
authors.lastname: Spicer
authors.forename: Sarita R
authors.initials: SR
authors.lastname: Shah
authors.forename: Allan M
authors.initials: AM
authors.lastname: Henslee
authors.forename: Brendan M
authors.initials: BM
authors.lastname: Watson
authors.forename: Lucas A
authors.initials: LA
authors.lastname: Kinard
authors.forename: James D
authors.initials: JD
authors.lastname: Kretlow
authors.forename: Kristin
authors.initials: K
authors.lastname: Bevil
authors.forename: Lauren
authors.initials: L
authors.lastname: Kattchee
authors.forename: George N
authors.initials: GN
authors.lastname: Bennett
authors.forename: Nagi
authors.initials: N
authors.lastname: Demian
authors.forename: Katrin
authors.initials: K
authors.lastname: Mende
authors.forename: Clinton K
authors.initials: CK
authors.lastname: Murray
authors.forename: John A
authors.initials: JA
authors.lastname: Jansen
authors.forename: Mark E
authors.initials: ME
authors.lastname: Wong
authors.forename: Antonios G
authors.initials: AG
authors.lastname: Mikos
authors.forename: F Kurtis
authors.initials: FK
authors.lastname: Kasper
mesh_terms: D000150:Acinetobacter; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology / Q000627:therapeutic use*; D001424:Bacterial Infections / Q000097:blood / Q000188:drug therapy* / Q000382:microbiology* / Q000503:physiopathology; D001806:Blood Urea Nitrogen; D003091:Colistin / Q000494:pharmacology / Q000627:therapeutic use*; D003404:Creatinine / Q000097:blood; D004195:Disease Models, Animal; D006801:Humans; D007677:Kidney Function Tests; D008297:Male; D008334:Mandible / Q000187:drug effects / Q000382:microbiology* / Q000473:pathology* / Q000601:surgery; D008826:Microbial Sensitivity Tests; D009061:Mouth Mucosa / Q000187:drug effects / Q000382:microbiology / Q000473:pathology / Q000601:surgery; D019904:Polymethyl Methacrylate / Q000737:chemistry*; D016062:Porosity; D019736:Prostheses and Implants; D011817:Rabbits
chemical_list: D000900:Anti-Bacterial Agents; D019904:Polymethyl Methacrylate; D003404:Creatinine; D003091:Colistin
keywords: Antibiotic delivery; Composite tissue defect; Craniofacial reconstruction; Infected defect; Space maintainer