title: Nerve regeneration across cryopreserved allografts from cadaveric donors: a novel approach for peripheral nerve reconstruction.
abstract: OBJECTIVE
  The use of allografts from cadaveric donors has attracted renewed interest in recent years, and pretreatment with cryopreservation and immunosuppression methods has been investigated to maximize axonal regrowth and minimize allograft rejection. The authors wanted to assess the outcome of treatments of brachial plexus stretch injuries with cryopreserved allografts from cadaveric donors in nonimmunosuppressed patients.
  
  
  METHODS
  Ten patients with brachial plexus lesions were submitted to electromyography (EMG) testing 1 and 3 months after a traumatic event and 1 week before surgery to localize and identify the type of lesion. Intraoperative EMG recordings were performed for intraoperative monitoring to select the best surgical strategy, and postoperative EMG was used to follow up patients and determine surgical outcomes. If nerve action potentials (NAPs) were present intraoperatively, neurolysis was performed, whereas muscular/nerve neurotization was performed if NAPs were absent. Cryopreserved allografts obtained from selected cadaveric donors and provided by the tissue bank of Treviso were used for nerve reconstruction in patients who were not treated with immunosuppressive drugs.
  
  
  RESULTS
  The surgical strategy was selected according to the type and site of the nerve lesion and on the basis of IOM results: 14 cryopreserved allografts were used for 7 muscular neurotizations and for 7 nerve neurotizations, and 5 neurolysis procedures were performed. All of the patients had regained motor function at the 1- and 2-year follow-ups.
  
  
  CONCLUSIONS
  Some variables may affect functional recovery after allograft surgery, and the outcome of peripheral nerve reconstruction is more favorable when patients are carefully evaluated and selected for the surgery. The authors demonstrated that using cryopreserved allografts from cadaveric donors is a valid surgical strategy to restore function of the damaged nerve without the need for any immunosuppressive treatments. This approach offers new perspectives on procedures for extensive reconstruction of brachial and lumbosacral plexuses.
authors.affiliation: Unità Operativa Neurologia, Azienda Ospedaliera Universitaria Integrata, Verona;
authors.forename: Giovanna
authors.initials: G
authors.lastname: Squintani
authors.forename: Bruno
authors.initials: B
authors.lastname: Bonetti
authors.forename: Adolfo
authors.initials: A
authors.lastname: Paolin
authors.forename: Daniela
authors.initials: D
authors.lastname: Vici
authors.forename: Elisa
authors.initials: E
authors.lastname: Cogliati
authors.forename: Bruno
authors.initials: B
authors.lastname: Murer
authors.forename: Giorgio
authors.initials: G
authors.lastname: Stevanato
mesh_terms: D064591:Allografts; D001917:Brachial Plexus / Q000293:injuries / Q000503:physiopathology / Q000601:surgery*; D020516:Brachial Plexus Neuropathies / Q000503:physiopathology / Q000601:surgery*; D015925:Cryopreservation; D004576:Electromyography; D006801:Humans; D009416:Nerve Regeneration / Q000502:physiology*; D016067:Nerve Transfer / Q000379:methods*; D019651:Plastic Surgery Procedures; D020127:Recovery of Function / Q000502:physiology*