title: Occurrence and potential causes of androgenic activities in source and drinking water in China.
abstract: The increased incidences of disorders of male reproductive tract as well as testicular and prostate cancers have been attributed to androgenic pollutants in the environment. Drinking water is one pathway of exposure through which humans can be exposed. In this study, both potencies of androgen receptor (AR) agonists and antagonists were determined in organic extracts of raw source water as well as finished water from waterworks, tap water, boiled water, and poured boiled water in eastern China. Ten of 13 samples of source water exhibited detectable AR antagonistic potencies with AR antagonist equivalents (Ant-AR-EQs) ranging from <15.3 (detection limit) to 140 μg flutamide/L. However, no AR agonistic activity was detected in any source water. All finished water from waterworks, tap water, boiled water, and poured boiled water exhibited neither AR agonistic nor antagonistic activity. Although potential risks are posed by source water, water treatment processes effectively removed AR antagonists. Boiling and pouring of water further removed these pollutants. Phthalate esters (PAEs) including diisobutyl phthalate (DIBP) and dibutyl phthalate (DBP) were identified as major contributors to AR antagonistic potencies in source waters. Metabolites of PAEs exhibited no AR antagonistic activity and did not increase potencies of PAEs when they coexist.
authors.affiliation: State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University , Nanjing, People's Republic of China.
authors.forename: Xinxin
authors.initials: X
authors.lastname: Hu
authors.forename: Wei
authors.initials: W
authors.lastname: Shi
authors.forename: Si
authors.initials: S
authors.lastname: Wei
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Zhang
authors.forename: Jianfang
authors.initials: J
authors.lastname: Feng
authors.forename: Guanjiu
authors.initials: G
authors.lastname: Hu
authors.forename: Sulan
authors.initials: S
authors.lastname: Chen
authors.forename: John P
authors.initials: JP
authors.lastname: Giesy
authors.forename: Hongxia
authors.initials: H
authors.lastname: Yu
mesh_terms: D000726:Androgen Antagonists / Q000032:analysis*; D000728:Androgens / Q000032:analysis; D045744:Cell Line, Tumor; D002681:China; D060766:Drinking Water / Q000032:analysis*; D004784:Environmental Monitoring; D006801:Humans; D010575:Pesticides / Q000032:analysis; D010636:Phenols / Q000032:analysis; D010795:Phthalic Acids / Q000032:analysis; D014874:Water Pollutants, Chemical / Q000032:analysis*
chemical_list: D000726:Androgen Antagonists; D000728:Androgens; D060766:Drinking Water; D010575:Pesticides; D010636:Phenols; D010795:Phthalic Acids; D014874:Water Pollutants, Chemical