title: Fabrication and characterization of scaffold from cadaver goat-lung tissue for skin tissue engineering applications.
abstract: The present study aims to fabricate scaffold from cadaver goat-lung tissue and evaluate it for skin tissue engineering applications. Decellularized goat-lung scaffold was fabricated by removing cells from cadaver goat-lung tissue enzymatically, to have cell-free 3D-architecture of natural extracellular matrix. DNA quantification assay and Hematoxylin and eosin staining confirmed the absence of cellular material in the decellularized lung-tissue. SEM analysis of decellularized scaffold shows the intrinsic porous structure of lung tissue with well-preserved pore-to-pore interconnectivity. FTIR analysis confirmed non-denaturation and well maintainance of collagenous protein structure of decellularized scaffold. MTT assay, SEM analysis and H&E staining of human skin-derived Mesenchymal Stem cell, seeded over the decellularized scaffold, confirms stem cell attachment, viability, biocompatibility and proliferation over the decellularized scaffold. Expression of Keratin18 gene, along with CD105, CD73 and CD44, by human skin-derived Mesenchymal Stem cells over decellularized scaffold signifies that the cells are viable, proliferating and migrating, and have maintained their critical cellular functions in the presence of scaffold. Thus, overall study proves the applicability of the goat-lung tissue derived decellularized scaffold for skin tissue engineering applications.
authors.affiliation: Department of Polymer and Process Engineering, Indian Institute of Technology, Roorkee, India.
authors.forename: Sweta K
authors.initials: SK
authors.lastname: Gupta
authors.forename: Amit K
authors.initials: AK
authors.lastname: Dinda
authors.forename: Pravin D
authors.initials: PD
authors.lastname: Potdar
authors.forename: Narayan C
authors.initials: NC
authors.lastname: Mishra
mesh_terms: D000818:Animals; D002102:Cadaver; D002448:Cell Adhesion; D049109:Cell Proliferation; D002470:Cell Survival; D004247:DNA / Q000378:metabolism; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006041:Goats; D006801:Humans; D008168:Lung / Q000166:cytology* / Q000648:ultrastructure; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism; D015203:Reproducibility of Results; D012867:Skin / Q000166:cytology*; D017550:Spectroscopy, Fourier Transform Infrared; D013194:Staining and Labeling; D013242:Sterilization; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D004247:DNA
keywords: Decellularization; Goat-lung; Scaffold; Skin; Stem cell; Tissue engineering