title: Characterizing the normal proteome of human ciliary body.
abstract: BACKGROUND
  The ciliary body is the circumferential muscular tissue located just behind the iris in the anterior chamber of the eye. It plays a pivotal role in the production of aqueous humor, maintenance of the lens zonules and accommodation by changing the shape of the crystalline lens. The ciliary body is the major target of drugs against glaucoma as its inhibition leads to a drop in intraocular pressure. A molecular study of the ciliary body could provide a better understanding about the pathophysiological processes that occur in glaucoma. Thus far, no large-scale proteomic investigation has been reported for the human ciliary body.
  
  
  RESULTS
  In this study, we have carried out an in-depth LC-MS/MS-based proteomic analysis of normal human ciliary body and have identified 2,815 proteins. We identified a number of proteins that were previously not described in the ciliary body including importin 5 (IPO5), atlastin-2 (ATL2), B-cell receptor associated protein 29 (BCAP29), basigin (BSG), calpain-1 (CAPN1), copine 6 (CPNE6), fibulin 1 (FBLN1) and galectin 1 (LGALS1). We compared the plasma proteome with the ciliary body proteome and found that the large majority of proteins in the ciliary body were also detectable in the plasma while 896 proteins were unique to the ciliary body. We also classified proteins using pathway enrichment analysis and found most of proteins associated with ubiquitin pathway, EIF2 signaling, glycolysis and gluconeogenesis.
  
  
  CONCLUSIONS
  More than 95% of the identified proteins have not been previously described in the ciliary body proteome. This is the largest catalogue of proteins reported thus far in the ciliary body that should provide new insights into our understanding of the factors involved in maintaining the secretion of aqueous humor. The identification of these proteins will aid in understanding various eye diseases of the anterior segment such as glaucoma and presbyopia.
authors.affiliation: Institute of Bioinformatics, International Technology Park, Bangalore 560 066, India.|Department of Biotechnology, Kuvempu University, Shankaraghatta, Shimoga 577 451, Karnataka, India.
authors.forename: Renu
authors.initials: R
authors.lastname: Goel
authors.affiliation: Institute of Bioinformatics, International Technology Park, Bangalore 560 066, India.|Amrita School of Biotechnology, Amrita Vishwa Vidyapeetham, Kollam 690 525, Kerala, India.|Vittala International Institute Of Ophthalmology, Bangalore 560 085, Karnataka, India.
authors.forename: Krishna R
authors.initials: KR
authors.lastname: Murthy
authors.affiliation: Institute of Bioinformatics, International Technology Park, Bangalore 560 066, India.|Centre of Excellence in Bioinformatics, Bioinformatics Centre, School of Life Sciences, Pondicherry University, Puducherry 605 014, India.
authors.forename: Srinivas M
authors.initials: SM
authors.lastname: Srikanth
authors.affiliation: Institute of Bioinformatics, International Technology Park, Bangalore 560 066, India.|Manipal University, Madhav Nagar, Manipal 576104, Karnataka, India.
authors.forename: Sneha M
authors.initials: SM
authors.lastname: Pinto
authors.affiliation: Institute of Bioinformatics, International Technology Park, Bangalore 560 066, India.|Amrita School of Biotechnology, Amrita Vishwa Vidyapeetham, Kollam 690 525, Kerala, India.
authors.forename: Mitali
authors.initials: M
authors.lastname: Bhattacharjee
authors.affiliation: Institute of Bioinformatics, International Technology Park, Bangalore 560 066, India.|Amrita School of Biotechnology, Amrita Vishwa Vidyapeetham, Kollam 690 525, Kerala, India.
authors.forename: Dhanashree S
authors.initials: DS
authors.lastname: Kelkar
authors.affiliation: Institute of Bioinformatics, International Technology Park, Bangalore 560 066, India.
authors.forename: Anil K
authors.initials: AK
authors.lastname: Madugundu
authors.affiliation: Institute of Bioinformatics, International Technology Park, Bangalore 560 066, India.
authors.forename: Gourav
authors.initials: G
authors.lastname: Dey
authors.affiliation: Institute of Bioinformatics, International Technology Park, Bangalore 560 066, India.|Department of Biotechnology, Kuvempu University, Shankaraghatta, Shimoga 577 451, Karnataka, India.|Research Unit for Immunoinformatics, RIKEN Research Center for Allergy and Immunology, RIKEN Yokohama Institute, Kanagawa 230 0045, Japan.
authors.forename: Sujatha S
authors.initials: SS
authors.lastname: Mohan
authors.affiliation: Department of Biotechnology, Kuvempu University, Shankaraghatta, Shimoga 577 451, Karnataka, India.
authors.forename: Venkatarangaiah
authors.initials: V
authors.lastname: Krishna
authors.affiliation: Institute of Bioinformatics, International Technology Park, Bangalore 560 066, India.|Amrita School of Biotechnology, Amrita Vishwa Vidyapeetham, Kollam 690 525, Kerala, India.|Manipal University, Madhav Nagar, Manipal 576104, Karnataka, India.
authors.forename: Ts Keshava
authors.initials: TK
authors.lastname: Prasad
authors.affiliation: Johns Hopkins University School of Medicine, Baltimore 21205, MD, USA.|Department of Cell Biology, Johns Hopkins School of Medicine, Baltimore, MD, USA.|Department of Ophthalmology, Johns Hopkins School of Medicine, Baltimore, MD, USA.
authors.forename: Shukti
authors.initials: S
authors.lastname: Chakravarti
authors.affiliation: Institute of Bioinformatics, International Technology Park, Bangalore 560 066, India.
authors.forename: H C
authors.initials: HC
authors.lastname: Harsha
authors.affiliation: Johns Hopkins University School of Medicine, Baltimore 21205, MD, USA.|McKusick-Nathans Institute of Genetic Medicine, Departments of Biological Chemistry, Oncology and Pathology, Johns Hopkins University School of Medicine, Baltimore 21205, MD, USA.
authors.forename: Akhilesh
authors.initials: A
authors.lastname: Pandey
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