title: Salmonella enterica serovar Typhimurium BaeSR two-component system positively regulates sodA in response to ciprofloxacin.
abstract: In response to antibiotics, bacteria activate regulatory systems that control the expression of genes that participate in detoxifying these compounds, like multidrug efflux systems. We previously demonstrated that the BaeSR two-component system from Salmonella enterica serovar Typhimurium (S. Typhimurium) participates in the detection of ciprofloxacin, a bactericidal antibiotic, and in the positive regulation of mdtA, an efflux pump implicated in antibiotic resistance. In the present work, we provide further evidence for a role of the S. Typhimurium BaeSR two-component system in response to ciprofloxacin treatment and show that it regulates sodA expression. We demonstrate that, in the absence of BaeSR, the transcript levels of sodA and the activity of its gene product are lower. Using electrophoretic mobility shift assays and transcriptional fusions, we demonstrate that BaeR regulates sodA by a direct interaction with the promoter region.
authors.affiliation: Laboratorio de Microbiología Molecular, Departamento de Ciencias Biológicas, Facultad de Ciencias Biológicas, Universidad Andres Bello, Santiago, Chile.
authors.forename: P
authors.initials: P
authors.lastname: Guerrero
authors.affiliation: Laboratorio de Microbiología Molecular, Departamento de Ciencias Biológicas, Facultad de Ciencias Biológicas, Universidad Andres Bello, Santiago, Chile.
authors.forename: B
authors.initials: B
authors.lastname: Collao
authors.affiliation: Laboratorio de Microbiología Molecular, Departamento de Ciencias Biológicas, Facultad de Ciencias Biológicas, Universidad Andres Bello, Santiago, Chile.
authors.forename: R
authors.initials: R
authors.lastname: Álvarez
authors.affiliation: Laboratorio de Microbiología Molecular, Departamento de Ciencias Biológicas, Facultad de Ciencias Biológicas, Universidad Andres Bello, Santiago, Chile.
authors.forename: H
authors.initials: H
authors.lastname: Salinas
authors.affiliation: DOE Great Lakes Bioenergy Research Center, University of Wisconsin-Madison, USA.|Department of Biomolecular Chemistry, University of Wisconsin-Madison, USA.
authors.forename: E H
authors.initials: EH
authors.lastname: Morales
authors.affiliation: Laboratorio de Microbiología Molecular, Departamento de Ciencias Biológicas, Facultad de Ciencias Biológicas, Universidad Andres Bello, Santiago, Chile.
authors.forename: I L
authors.initials: IL
authors.lastname: Calderón
authors.affiliation: Laboratorio de Microbiología Molecular, Departamento de Ciencias Biológicas, Facultad de Ciencias Biológicas, Universidad Andres Bello, Santiago, Chile.
authors.forename: C P
authors.initials: CP
authors.lastname: Saavedra
authors.affiliation: Laboratorio de Microbiología Molecular, Departamento de Ciencias Biológicas, Facultad de Ciencias Biológicas, Universidad Andres Bello, Santiago, Chile.
authors.forename: F
authors.initials: F
authors.lastname: Gil
mesh_terms: D000900:Anti-Bacterial Agents / Q000378:metabolism*; D019677:Artificial Gene Fusion; D001426:Bacterial Proteins / Q000096:biosynthesis*; D002939:Ciprofloxacin / Q000378:metabolism*; D004269:DNA, Bacterial / Q000378:metabolism; D024202:Electrophoretic Mobility Shift Assay; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial; D055786:Gene Knockout Techniques; D027425:ATP-Binding Cassette, Sub-Family C Proteins / Q000235:genetics / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D011494:Protein Kinases / Q000235:genetics / Q000378:metabolism*; D012486:Salmonella typhimurium / Q000187:drug effects*; D013482:Superoxide Dismutase / Q000096:biosynthesis*; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D002939:Ciprofloxacin; D004269:DNA, Bacterial; D027425:ATP-Binding Cassette, Sub-Family C Proteins; D011494:Protein Kinases; D013482:Superoxide Dismutase; D015534:Trans-Activators; C525553:BaeR protein, Salmonella typhimurium; C095500:SodA protein, Bacteria; C525552:BaeS protein, Salmonella typhimurium
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