title: Differential expression patterns in chemosensory and non-chemosensory tissues of putative chemosensory genes identified by transcriptome analysis of insect pest the purple stem borer Sesamia inferens (Walker).
abstract: BACKGROUND
  A large number of insect chemosensory genes from different gene subfamilies have been identified and annotated, but their functional diversity and complexity are largely unknown. A systemic examination of expression patterns in chemosensory organs could provide important information.
  
  
  RESULTS
  We identified 92 putative chemosensory genes by analysing the transcriptome of the antennae and female sex pheromone gland of the purple stem borer Sesamia inferens, among them 87 are novel in this species, including 24 transcripts encoding for odorant binding proteins (OBPs), 24 for chemosensory proteins (CSPs), 2 for sensory neuron membrane proteins (SNMPs), 39 for odorant receptors (ORs) and 3 for ionotropic receptors (IRs). The transcriptome analyses were validated and quantified with a detailed global expression profiling by Reverse Transcription-PCR for all 92 transcripts and by Quantitative Real Time RT-PCR for selected 16 ones. Among the chemosensory gene subfamilies, CSP transcripts are most widely and evenly expressed in different tissues and stages, OBP transcripts showed a clear antenna bias and most of OR transcripts are only detected in adult antennae. Our results also revealed that some OR transcripts, such as the transcripts of SNMP2 and 2 IRs were expressed in non-chemosensory tissues, and some CSP transcripts were antenna-biased expression. Furthermore, no chemosensory transcript is specific to female sex pheromone gland and very few are found in the heads.
  
  
  CONCLUSIONS
  Our study revealed that there are a large number of chemosensory genes expressed in S. inferens, and some of them displayed unusual expression profile in non-chemosensory tissues. The identification of a large set of putative chemosensory genes of each subfamily from a single insect species, together with their different expression profiles provide further information in understanding the functions of these chemosensory genes in S. inferens as well as other insects.
authors.affiliation: Education Ministry, Key Laboratory of Integrated Management of Crop Diseases and Pests, College of Plant Protection, Nanjing Agricultural University, Nanjing, China.
authors.forename: Ya-Nan
authors.initials: YN
authors.lastname: Zhang
authors.forename: Jun-Yan
authors.initials: JY
authors.lastname: Jin
authors.forename: Rong
authors.initials: R
authors.lastname: Jin
authors.forename: Yi-Han
authors.initials: YH
authors.lastname: Xia
authors.forename: Jing-Jiang
authors.initials: JJ
authors.lastname: Zhou
authors.forename: Jian-Yu
authors.initials: JY
authors.lastname: Deng
authors.forename: Shuang-Lin
authors.initials: SL
authors.lastname: Dong
mesh_terms: D000818:Animals; D019476:Insect Proteins / Q000235:genetics; D007915:Lepidoptera / Q000235:genetics / Q000378:metabolism*; D018035:Receptors, Odorant / Q000235:genetics; D043563:Receptors, Pheromone / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D019476:Insect Proteins; D018035:Receptors, Odorant; D043563:Receptors, Pheromone; C045648:odorant-binding protein
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