title: Effectiveness of a new method of disinfecting the root canal, using Er, Cr:YSGG laser to kill Enterococcus faecalis in an infected tooth model.
abstract: Some lasers have demonstrated to provide effective disinfection when used as adjunctive device to the conventional treatment. The aim of this in vitro study was to determine the effectiveness of the erbium, chromium:yttrium scandium gallium garnet (Er, Cr:YSGG) laser by measuring its bactericidal effect inside the root canal experimentally colonized with Enterococcus faecalis. The laser was tested at different irradiation times (30 and 60 s) and energy of impulses (75 and 25 mJ). A total of 52 single-rooted extracted human teeth were endodontically prepared with rotary instrumentation. All were sterilized and inoculated with a suspension of E. faecalis (105 bacteria/ml). The teeth were randomized into three treatment (group 1, group 2, and group 3) and one control groups. In all groups, teeth were chemically irrigated with 5.25% sodium hypochlorite and 17% ethylenediaminetetraacetic acid. Groups 1 and 2 were also irradiated at 30 and 60 s, respectively, with an Er, Cr:YSGG laser at 75 mJ. Teeth of group 3 were treated with laser for 60 s at 25 mJ. Samples were processed to detect the presence of E. faecalis. For all groups, a bactericidal effect was observed. The use of laser at 75 mJ with an irradiation time of 30 and 60 s eliminated a percentage of 92.3 and 100% of E. faecalis, respectively. In the control group, a reduction of 92.3% was observed. Lower percentage of reduction (46.1%) was obtained in teeth treated with laser at 25 mJ for 60 s. No statistical differences were observed between the groups (P = 0.543, Fisher's exact test). The results indicated a bactericidal effect of Er, Cr:YSGG laser irradiation at the settings used in this study. The highest bactericidal effect of this laser was observed at 60 s of irradiation time, using an energy pulse of 75 mJ.
authors.affiliation: Dipartimento delle Discipline Chirurgiche, Oncologiche e Stomatologiche (Di.Chir.On.S), University of Palermo, Via del Vespro 129, 90127, Palermo, Italy, licata@odonto.unipa.it.
authors.forename: M E
authors.initials: ME
authors.lastname: Licata
authors.forename: A
authors.initials: A
authors.lastname: Albanese
authors.forename: G
authors.initials: G
authors.lastname: Campisi
authors.forename: D M
authors.initials: DM
authors.lastname: Geraci
authors.forename: R
authors.initials: R
authors.lastname: Russo
authors.forename: G
authors.initials: G
authors.lastname: Gallina
mesh_terms: D003731:Dental Caries / Q000601:surgery; D003786:Dental Pulp Cavity / Q000601:surgery*; D004202:Disinfectants / Q000737:chemistry*; D004203:Disinfection / Q000379:methods; D013293:Enterococcus faecalis; D004871:Erbium; D016908:Gram-Positive Bacterial Infections / Q000601:surgery*; D006801:Humans; D007834:Lasers; D053844:Lasers, Solid-State / Q000627:therapeutic use*; D003765:Models, Dental; D010510:Periodontal Diseases / Q000601:surgery; D012390:Root Canal Therapy / Q000379:methods; D012973:Sodium Hypochlorite / Q000737:chemistry; D014092:Tooth Root / Q000601:surgery
chemical_list: D004202:Disinfectants; D004871:Erbium; D012973:Sodium Hypochlorite
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