title: Phagocyte dysfunction in polymyalgia rheumatica and other age-related, chronic, inflammatory conditions.
abstract: This study was conducted to evaluate phagocyte function in patients with age-related chronic inflammatory conditions. It included 95 patients with PMR, 17 with GCA, 40 with EORA, and 25 age-matched HCs. Serum IL-8 was determined with a bead array. The chemotactic capacity, phagocytic ability, and oxidative burst activity of circulating leukocytes were determined with flow cytometry kits. Patients with active chronic inflammatory diseases showed a significant increase in circulating levels of IL-8 that remained elevated in patients with PMR or EORA, despite treatment. No correlation was found between circulating IL-8 and the migratory capacity of neutrophils. Neutrophils from patients with active EORA without stimulus and after fMLP stimuli showed a higher capacity to migrate than those of the HCs (P=0.033). The phagocytic activity of granulocytes in the patients with GCA was significantly higher than in the HCs and the patients with PMR or EORA (P<0.05). The percentage and MFI of phagocytes that produce ROIs when stimulated with Escherichia coli was significantly reduced in neutrophils and monocytes from the patients with age-restricted inflammatory conditions. We concluded that the effector functions of phagocytes, determined to be chemotaxis, phagocytosis, and oxidative burst, are deregulated in age-restricted inflammatory disorders and may have a pathogenic role.
authors.affiliation: 1.Facultad de Medicina. Universidad de Cantabria, Avda. Valdecilla s/n. 39008. Santander, Spain. vmartinezt@medynet.com.
authors.forename: Lorena
authors.initials: L
authors.lastname: Álvarez-Rodríguez
authors.forename: Marcos
authors.initials: M
authors.lastname: López-Hoyos
authors.forename: Jaime
authors.initials: J
authors.lastname: Calvo-Alén
authors.forename: Elena
authors.initials: E
authors.lastname: Aurrecoechea
authors.forename: Ignacio
authors.initials: I
authors.lastname: Villa
authors.forename: Víctor Manuel
authors.initials: VM
authors.lastname: Martínez-Taboada
mesh_terms: D000210:Acute-Phase Reaction; D000367:Age Factors; D000368:Aged; D000369:Aged, 80 and over; D001172:Arthritis, Rheumatoid / Q000276:immunology*; D002634:Chemotaxis, Leukocyte; D002908:Chronic Disease; D005260:Female; D013700:Giant Cell Arteritis / Q000276:immunology*; D006801:Humans; D016209:Interleukin-8 / Q000097:blood; D008297:Male; D008875:Middle Aged; D009504:Neutrophils / Q000276:immunology; D010586:Phagocytes / Q000276:immunology*; D011111:Polymyalgia Rheumatica / Q000276:immunology*; D016897:Respiratory Burst
chemical_list: D016209:Interleukin-8
keywords: aging; burst; innate immunity; interleukin 8; phagocytosis; rheumatoid arthritis