title: Fluorescence spectroscopy: a new approach in cervical cancer.
abstract: OBJECTIVE
  To study the diagnostic potential of fluorescence spectroscopy and its comparison with different screening methods, including Pap smear and colposcopy, in detecting early cervical neoplasia.
  
  
  METHODS
  The study was conducted on patients with gynecological complaints. A full gynecological workup of the patients was done along with Pap smear and colposcopy. Cervical biopsy was done in suspected cases and fresh tissue was sent to IIT for spectroscopy.
  
  
  RESULTS
  There is a definite increase in NADH fluorescence (67.4 %) and a decrease in collagen fluorescence (74 %) in dysplastic tissues. When epithelial fluorescence and stromal fluorescence are considered together, diagnostic accuracy is increased to 96.5 %.
  
  
  CONCLUSIONS
  The clinical diagnosis of cervical neoplasia by spectroscopic methods is potentially a reliable, fast, and cost-effective alternative to the conventional smear test which needs trained personnel for its interpretation. Research is still continuing to obtain a statistically significant cutoff value from in vitro studies and then use them for in vivo study.
authors.affiliation: Department of Obstetrics & Gynaecology, G S V M Medical College, New Type IV/7, Medical College Campus, Kanpur, 208 002 India.
authors.forename: Kiran
authors.initials: K
authors.lastname: Pandey
authors.forename: Asima
authors.initials: A
authors.lastname: Pradhan
authors.forename: Asha
authors.initials: A
authors.lastname: Agarwal
authors.forename: Ajay
authors.initials: A
authors.lastname: Bhagoliwal
authors.forename: Nidhi
authors.initials: N
authors.lastname: Agarwal
keywords: Cervical cancer; Fluorescence spectroscopy; Nicotinamide adenine dinucleotide dehydrogenase (NADH)
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