title: 3D spectral imaging with synchrotron Fourier transform infrared spectro-microtomography.
abstract: We report Fourier transform infrared spectro-microtomography, a nondestructive three-dimensional imaging approach that reveals the distribution of distinctive chemical compositions throughout an intact biological or materials sample. The method combines mid-infrared absorption contrast with computed tomographic data acquisition and reconstruction to enhance chemical and morphological localization by determining a complete infrared spectrum for every voxel (millions of spectra determined per sample).
authors.affiliation: Advanced Light Source Division, Lawrence Berkeley National Laboratory, Berkeley, California, USA. MCMartin@lbl.gov
authors.forename: Michael C
authors.identifier: 0000-0002-6611-070X
authors.initials: MC
authors.lastname: Martin
authors.forename: Charlotte
authors.initials: C
authors.lastname: Dabat-Blondeau
authors.forename: Miriam
authors.initials: M
authors.lastname: Unger
authors.forename: Julia
authors.initials: J
authors.lastname: Sedlmair
authors.forename: Dilworth Y
authors.initials: DY
authors.lastname: Parkinson
authors.forename: Hans A
authors.initials: HA
authors.lastname: Bechtel
authors.forename: Barbara
authors.initials: B
authors.lastname: Illman
authors.forename: Jonathan M
authors.initials: JM
authors.lastname: Castro
authors.forename: Marco
authors.initials: M
authors.lastname: Keiluweit
authors.forename: David
authors.initials: D
authors.lastname: Buschke
authors.forename: Brenda
authors.initials: B
authors.lastname: Ogle
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Nasse
authors.forename: Carol J
authors.initials: CJ
authors.lastname: Hirschmugl
mesh_terms: D000818:Animals; D053595:Embryonic Stem Cells / Q000648:ultrastructure; D006197:Hair / Q000648:ultrastructure; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D021621:Imaging, Three-Dimensional; D051379:Mice; D032107:Populus / Q000648:ultrastructure; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*; D017356:Synchrotrons; D014934:Wood / Q000648:ultrastructure; D055114:X-Ray Microtomography / Q000379:methods*
references.citation: J Biomed Opt. 2012 Nov;17(11):116013
references.pmid: 23117808
references.citation: IEEE Trans Med Imaging. 2000 Jun;19(6):601-15
references.pmid: 11026463
references.citation: Nat Methods. 2011 May;8(5):413-6
references.pmid: 21423192
references.citation: Science. 2010 Oct 8;330(6001):204-8
references.pmid: 20736401
references.citation: Anal Chem. 2010 Nov 1;82(21):8757-65
references.pmid: 20839782
references.citation: Phys Med Biol. 1999 Nov;44(11):2835-51
references.pmid: 10588288
references.citation: Cell. 2003 Oct 31;115(3):281-92
references.pmid: 14636556
references.citation: Neuroimage. 2012 Mar;60(1):376-83
references.pmid: 22197789
references.citation: Analyst. 2012 Oct 21;137(20):4774-84
references.pmid: 22946081
references.citation: Plant Physiol. 1980 Jan;65(1):57-60
references.pmid: 16661142
references.citation: Dev Dyn. 2012 May;241(5):953-64
references.pmid: 22434721
references.citation: Stem Cells. 2008 May;26(5):1146-54
references.pmid: 18308941
references.citation: Appl Spectrosc. 2012 May;66(5):475-91
references.pmid: 22524953
references.citation: Mech Dev. 1993 Nov;44(1):41-50
references.pmid: 8155574
references.citation: Analyst. 2010 Dec;135(12):3126-32
references.pmid: 20953512
references.citation: IEEE Trans Med Imaging. 1999 Sep;18(9):801-14
references.pmid: 10571385
references.citation: J Biol Phys. 2003 Jun;29(2-3):275-86
references.pmid: 23345845
references.citation: Nature. 2009 Oct 8;461(7265):780-3
references.pmid: 19812671
references.citation: Biochim Biophys Acta. 2006 Jul;1758(7):846-57
references.pmid: 16781664
references.citation: IEEE Trans Med Imaging. 1997 Apr;16(2):166-75
references.pmid: 9101326
references.citation: Nat Rev Mol Cell Biol. 2007 Oct;8(10):839-45
references.pmid: 17684528
references.citation: J Synchrotron Radiat. 2008 Jul;15(Pt 4):323-8
references.pmid: 18552422
references.citation: Plant Physiol. 2010 Sep;154(1):121-33
references.pmid: 20592039
references.citation: Faraday Discuss. 2004;126:289-302; discussion 303-11
references.pmid: 14992414
references.citation: Proc Natl Acad Sci U S A. 2010 Sep 7;107(36):15676-80
references.pmid: 20720164
references.citation: IEEE Trans Image Process. 1996;5(9):1346-58
references.pmid: 18285223