title: Improved thermal stability of lipase in W/O microemulsion by temperature-sensitive polymers.
abstract: Lipase is active at the water-oil interface and thus very useful for many applications in non-aqueous media. However, the use of lipase is often limited due to the heat inactivation which is mainly caused by the irreversible aggregation among lipase molecules. The temperature-sensitive polymers can spontaneously form complexes with lipases at higher temperature in the confined spaces of the water in oil microemulsion. With cooling, lipases are released from the complexes and refold into the native state. In this way, the thermal stability of lipase in a microemulsion is effectively improved, and so is the stability of lipase at ambient temperature. Apart from proving the effectiveness and generality of this method, the temperature-sensitive polymers/lipase microemulsion represents a simple and efficient system which could be used in practical applications, since lipase retains the interfacial activity in this system. Moreover, the influences of some factors on the improvement are discussed and the mechanism of this method is suggested after exploring the process by dynamic light scattering and fluorescence measurements.
authors.affiliation: Key Laboratory of Functional Polymer Materials, Ministry of Education, Institute of Polymer Chemistry, College of Chemistry, Nankai University, Tianjin 300071, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Tao
authors.affiliation: Key Laboratory of Functional Polymer Materials, Ministry of Education, Institute of Polymer Chemistry, College of Chemistry, Nankai University, Tianjin 300071, China.
authors.forename: Ang
authors.initials: A
authors.lastname: Li
authors.affiliation: Key Laboratory of Functional Polymer Materials, Ministry of Education, Institute of Polymer Chemistry, College of Chemistry, Nankai University, Tianjin 300071, China.
authors.forename: Xue
authors.initials: X
authors.lastname: Liu
authors.affiliation: Key Laboratory of Functional Polymer Materials, Ministry of Education, Institute of Polymer Chemistry, College of Chemistry, Nankai University, Tianjin 300071, China.
authors.forename: Hongjun
authors.initials: H
authors.lastname: Gao
authors.affiliation: Key Laboratory of Functional Polymer Materials, Ministry of Education, Institute of Polymer Chemistry, College of Chemistry, Nankai University, Tianjin 300071, China.
authors.forename: Zhenkun
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Functional Polymer Materials, Ministry of Education, Institute of Polymer Chemistry, College of Chemistry, Nankai University, Tianjin 300071, China.
authors.forename: Rujiang
authors.initials: R
authors.lastname: Ma
authors.affiliation: Key Laboratory of Functional Polymer Materials, Ministry of Education, Institute of Polymer Chemistry, College of Chemistry, Nankai University, Tianjin 300071, China.
authors.forename: Yingli
authors.initials: Y
authors.lastname: An
authors.affiliation: Key Laboratory of Functional Polymer Materials, Ministry of Education, Institute of Polymer Chemistry, College of Chemistry, Nankai University, Tianjin 300071, China. Electronic address: shilinqi@nankai.edu.cn.
authors.forename: Linqi
authors.initials: L
authors.lastname: Shi
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry; D002175:Candida / Q000201:enzymology; D004655:Emulsions; D004795:Enzyme Stability; D057446:Hydrodynamics; D008049:Lipase / Q000378:metabolism*; D009821:Oils / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013696:Temperature; D014867:Water / Q000737:chemistry*
chemical_list: D000180:Acrylic Resins; D004655:Emulsions; D009821:Oils; D011108:Polymers; D014867:Water; C052970:poly-N-isopropylacrylamide; D008049:Lipase
keywords: Interfacial activity; Lipase; Microemulsion; Temperature-sensitive polymer; Thermal stability