title: Sinorhodobacter ferrireducens gen. nov., sp. nov., a non-phototrophic iron-reducing bacterium closely related to phototrophic Rhodobacter species.
abstract: An Fe(III) oxide-reducing bacterium designated as SgZ-3(T), which could couple glucose oxidation to Fe(III) oxide reduction for energy conservation, was isolated from an electrochemical biofilm. The isolate was Gram-negative, catalase-positive and oxidase-negative, and facultative anaerobic. NaCl was not required for growth, but NaCl concentrations up to 5 % (w/v) were tolerated. Growth occurred in TSB (tryptic soy broth) at 20-40 °C (optimum 30 °C) and at pH 6.0-7.5 (optimum 7.0). Phototrophic growth could not be demonstrated. No vesicular photosynthetic membrane was observed. Bacteriochlorophyll α and carotenoids were absent. Biotin and thiamine were required as growth factors for the isolate. Phylogenetic analysis of the 16S rRNA gene sequence placed strain SgZ-3(T) within the family Rhodobacteraceae and affiliated with an phototrophic genera Rhodobacter. The G+C content of the genomic DNA was 68.6 mol%. Strain SgZ-3(T) contained Q-10 as the predominant quinone. The major cellular fatty acids were C18:1 ω6c and/or C18:1 ω7c (66.9 %) and C16:0 (9.5 %). Based on its phenotypic and phylogenetic properties, chemotaxonomic analysis and the results of physiological and biochemical tests, strain SgZ-3(T) (=KACC 16603(T) = CCTCC AB2012026(T)) was designated as the type strain of a new genus and a novel species of the family Rhodobacteraceae, for which the name Sinorhodobacter ferrireducens sp. nov. was proposed.
authors.affiliation: Guangdong Institute of Eco-Environmental and Soil Sciences, Guangzhou, 510650, People's Republic of China.
authors.forename: Guiqin
authors.initials: G
authors.lastname: Yang
authors.forename: Ming
authors.initials: M
authors.lastname: Chen
authors.forename: Shungui
authors.initials: S
authors.lastname: Zhou
authors.forename: Zhi
authors.initials: Z
authors.lastname: Liu
authors.forename: Yong
authors.initials: Y
authors.lastname: Yuan
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D002374:Catalase / Q000378:metabolism; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D004783:Environmental Microbiology; D005227:Fatty Acids / Q000032:analysis; D005947:Glucose / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007501:Iron / Q000378:metabolism*; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000378:metabolism; D052817:Phototrophic Processes; D010802:Phylogeny; D011809:Quinones / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D041902:Rhodobacteraceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride / Q000378:metabolism; D013696:Temperature
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D011809:Quinones; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride; D007501:Iron; D010088:Oxidoreductases; D002374:Catalase; D005947:Glucose