title: Classification of Achromobacter genogroups 2, 5, 7 and 14 as Achromobacter insuavis sp. nov., Achromobacter aegrifaciens sp. nov., Achromobacter anxifer sp. nov. and Achromobacter dolens sp. nov., respectively.
abstract: The phenotypic and genotypic characteristics of seventeen Achromobacter strains representing MLST genogroups 2, 5, 7 and 14 were examined. Although genogroup 2 and 14 strains shared a DNA-DNA hybridization level of about 70%, the type strains of both genogroups differed in numerous biochemical characteristics and all genogroup 2 and 14 strains could by distinguished by nitrite reduction, denitrification and growth on acetamide. Given the MLST sequence divergence which identified genogroups 2 and 14 as clearly distinct populations, the availability of nrdA sequence analysis as a single locus identification tool for all Achromobacter species and genogroups, and the differential phenotypic characteristics, we propose to formally classify Achromobacter genogroups 2, 5, 7 and 14 as four novel Achromobacter species for which we propose the names Achromobacter insuavis sp. nov. (with strain LMG 26845(T) [=CCUG 62426(T)] as the type strain), Achromobacter aegrifaciens sp. nov. (with strain LMG 26852(T) [=CCUG 62438(T)] as the type strain), Achromobacter anxifer sp. nov. (with strain LMG 26857(T) [=CCUG 62444(T)] as the type strain), and Achromobacter dolens sp. nov. (with strain LMG 26840(T) [=CCUG 62421(T)] as the type strain).
authors.affiliation: Laboratorium voor Microbiologie, Vakgroep Biochemie en microbiologie, Faculteit Wetenschappen, Universiteit Gent, Gent, Belgium. Electronic address: Peter.Vandamme@ugent.be.
authors.forename: Peter
authors.initials: P
authors.lastname: Vandamme
authors.forename: Edward R B
authors.initials: ER
authors.lastname: Moore
authors.forename: Margo
authors.initials: M
authors.lastname: Cnockaert
authors.forename: Charlotte
authors.initials: C
authors.lastname: Peeters
authors.forename: Liselott
authors.initials: L
authors.lastname: Svensson-Stadler
authors.forename: Kurt
authors.initials: K
authors.lastname: Houf
authors.forename: Theodore
authors.initials: T
authors.lastname: Spilker
authors.forename: John J
authors.initials: JJ
authors.lastname: LiPuma
mesh_terms: D042402:Achromobacter / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000502:physiology; D015373:Bacterial Typing Techniques; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D005838:Genotype; D008969:Molecular Sequence Data; D058885:Multilocus Sequence Typing; D010802:Phylogeny
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids
keywords: Achromobacter; Cystic fibrosis; Multilocus sequence analysis