title: Development of small diameter fibrous vascular grafts with outer wall multiscale architecture to improve cell penetration.
abstract: This work explains about the development of a unique tubular scaffold for vascular tissue engineering. The inner layer/layers was made up of aligned poly (lactic acid) (PLA) nano fibers and outer layers were composed of random multiscale fibers of poly(caprolactone) (PCL)/PLA providing larger pores for Smooth Muscle Cell (SMC) penetration. The fabricated scaffolds were characterized by SEM. Cell attachment and infiltration studies using SMCs on the multiscale fibers were characterized by SEM and confocal microscopy. Blood compatibility of the scaffold was analysed by haemolysis-coagulation assays, platelet activation studies and the effect of material/fiber alignment on the morphological stability of Red Blood Cells (RBCs) were evaluated using SEM. Since this hierarchically designed tubular scaffold closely mimics the morphology of native vessel, this could be a better candidate for vascular tissue engineering.
authors.affiliation: Amrita Centre for Nanosciences and Molecular Medicine, Amrita Institute of Medical Sciences and Research Centre, Amrita Vishwa Vidyapeetham University, Kochi 682041, Kerala, India.
authors.forename: K T
authors.initials: KT
authors.lastname: Shalumon
authors.forename: K P
authors.initials: KP
authors.lastname: Chennazhi
authors.forename: Shantikumar V
authors.initials: SV
authors.lastname: Nair
authors.forename: R
authors.initials: R
authors.lastname: Jayakumar
mesh_terms: D001807:Blood Vessel Prosthesis; D001808:Blood Vessels / Q000166:cytology / Q000254:growth & development*; D002478:Cells, Cultured; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006801:Humans; D032389:Myocytes, Smooth Muscle / Q000166:cytology* / Q000502:physiology*; D011108:Polymers / Q000737:chemistry*; D016062:Porosity; D023822:Tissue Engineering / Q000295:instrumentation*; D054457:Tissue Scaffolds
chemical_list: D011108:Polymers