title: [Heterologous expression and enzymatic analysis of Streptomyces griseus trypsin in Streptomyces lividans].
abstract: Trypsin as an important serine protease has been widely used in food, pharmaceutical and tanning industries. In this study, we successfully expressed trypsin (cloning from Streptomyces griseus ATCC10137) in Streptomyces lividans TK24 and comparatively investigated its enzymatic properties. Specifically, applying S. griseus ATCC 10137 genome as template, we obtained the sprT gene and sub-cloned it into the expression plasmid pIJ86, generating the recombinant strain S. lividans TK24/pIJ86-sprT. When cultivated in R2YE and SELF, the activity of rSGT reached 9.21 U/mL and 8.61 U/mL, respectively. Meanwhile, the results of the enzymatic analysis showed that rSGT exhibited a higher acid tolerance and a higher specificity to hydrolyze amide bonds compared with bovine trypsin (BT). In addition, Zn2+ and organic solvents up-regulated esterase and amidase of rSGT. Taken together, the results obtained herein provide meaningful information for further modification of rSGT and its industrial application.
authors.affiliation: Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, Jiangsu, China.
authors.forename: Tengbo
authors.initials: T
authors.lastname: Ma
authors.forename: Zhenmin
authors.initials: Z
authors.lastname: Ling
authors.forename: Zhen
authors.initials: Z
authors.lastname: Kang
authors.forename: Jianghua
authors.initials: J
authors.lastname: Li
authors.forename: Guocheng
authors.initials: G
authors.lastname: Du
authors.forename: Jian
authors.initials: J
authors.lastname: Chen
mesh_terms: D005285:Fermentation; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D013305:Streptomyces griseus / Q000201:enzymology*; D048170:Streptomyces lividans / Q000235:genetics / Q000378:metabolism*; D014357:Trypsin / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D011994:Recombinant Proteins; D014357:Trypsin