title: [Chuju total flavonoids control the SFRP4 expression in Wnt pathway in rheumatoid arthritis model rats].
abstract: OBJECTIVE
  To determine the effect of Chuju total flavonoids (CJTF) on the secreted frizzledrelated protein 4 (SFRP4) expression in Wnt pathway in rheumatoid arthritis (RA) model rats.
  
  
  METHODS
  The role of CJTF in the treatment of RA model rats was evaluated by rat arthritis score and paw edema score. The expression regulation of the SFRP4, β-catenin and C-myc in Wnt pathway in RA model rats was detected by RT-PCR and Western blot after CJTF gavage treatment.
  
  
  RESULTS
  After CJTF treatment, the rat arthritis score and paw edema score in RA model rats were significantly decreased when the RA model rats were treated with CJTF, the SFRP4 expression was significantly up-regulated, while the β-catenin and C-myc gene expression were significantly downregulated in RA model rat synovial tissues.
  
  
  CONCLUSIONS
  CJTF has significant therapeutic effect and inhibitory effect on Wnt pathway activation by targeting SFRP4 in RA model rat synovium.
authors.affiliation: Poultry Disease Monitoring Key Laboratory; Food and Drug College; Life Science College, Anhui Science and Technology University, Bengbu Anhui 233100, China fensheng1981@126.com.
authors.forename: Chenggui
authors.initials: C
authors.lastname: Miao
authors.forename: Jian
authors.initials: J
authors.lastname: Liu
authors.forename: Yonghe
authors.initials: Y
authors.lastname: Zhang
authors.forename: Min
authors.initials: M
authors.lastname: Gao
authors.forename: Qingyu
authors.initials: Q
authors.lastname: Chen
authors.forename: Huaqi
authors.initials: H
authors.lastname: He
mesh_terms: D000818:Animals; D001172:Arthritis, Rheumatoid / Q000188:drug therapy* / Q000378:metabolism; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000627:therapeutic use; D005419:Flavonoids / Q000302:isolation & purification / Q000627:therapeutic use*; D008297:Male; D011518:Proto-Oncogene Proteins / Q000235:genetics / Q000378:metabolism*; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013583:Synovial Membrane / Q000378:metabolism; D060449:Wnt Signaling Pathway / Q000187:drug effects*; D051176:beta Catenin / Q000235:genetics / Q000378:metabolism; D000094742:Secreted Frizzled-Related Proteins
chemical_list: D004365:Drugs, Chinese Herbal; D005419:Flavonoids; D011518:Proto-Oncogene Proteins; D016271:Proto-Oncogene Proteins c-myc; D012333:RNA, Messenger; D051176:beta Catenin; C495268:Ctnnb1 protein, rat; C498455:Sfrp4 protein, rat; D000094742:Secreted Frizzled-Related Proteins