title: Evaluation of antiplasmodial activity of green synthesized silver nanoparticles.
abstract: In the present study silver nanoparticles (silver(np)) were synthesized from AgNO3 through simple green routes using either purified Alpha Amylase or aqueous leaf extracts of Ashoka and Neem respectively. The use of plant extract/enzyme for synthesis of nanoparticles is a single-step, cost effective and eco-friendly process. The silver(np) obtained by these three different ways were characterized using UV-visible spectroscopy, DLS, TEM, XRD and FTIR. These nanoparticles were found to be antiplasmodial with IC50 (μg/ml) 3.75 (Amylase(np)), 8 (Ashoka(np)) and 30 (Neem(np)) whereas plant extracts or amylase alone did not show any activity up to 40 μg/ml. Although AgNO3 was also found to have intrinsic antiplasmodial activity (IC50 0.5 μg/ml), the hemolytic tendencies appeared to be higher for AgNO3 (MHC10: 10 μg/ml) against the nanoparticulate preparations (MHC10: >40 μg/ml).
authors.affiliation: Department of Biosciences, Jamia Millia Islamia, New Delhi 110025, India.
authors.forename: Abhijeet
authors.initials: A
authors.lastname: Mishra
authors.affiliation: Malaria Research Group, International Centre for Genetic Engineering and Biotechnology, New Delhi 110067, India.
authors.forename: Naveen Kumar
authors.initials: NK
authors.lastname: Kaushik
authors.affiliation: Department of Biosciences, Jamia Millia Islamia, New Delhi 110025, India. Electronic address: msardar@jmi.ac.in.
authors.forename: Meryam
authors.initials: M
authors.lastname: Sardar
authors.affiliation: Malaria Research Group, International Centre for Genetic Engineering and Biotechnology, New Delhi 110067, India. Electronic address: dinkar@icgeb.res.in.
authors.forename: Dinkar
authors.initials: D
authors.lastname: Sahal
mesh_terms: D000681:Amylases / Q000378:metabolism; D000818:Animals; D000962:Antimalarials / Q000494:pharmacology*; D055772:Green Chemistry Technology / Q000379:methods*; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010936:Plant Extracts / Q000494:pharmacology; D018515:Plant Leaves / Q000737:chemistry; D010963:Plasmodium falciparum / Q000187:drug effects / Q000254:growth & development; D012834:Silver / Q000737:chemistry* / Q000494:pharmacology*
chemical_list: D000962:Antimalarials; D010936:Plant Extracts; D012834:Silver; D000681:Amylases
keywords: Antimalarial activity; Green synthesis; Silver nanoparticles