title: The RNA chaperone and protein chaperone activity of Arabidopsis glycine-rich RNA-binding protein 4 and 7 is determined by the propensity for the formation of high molecular weight complexes.
abstract: RNA chaperones and protein chaperones are cellular proteins that can aid the correct folding of target RNAs and proteins, respectively. Although many proteins possessing RNA chaperone or protein chaperone activity have been demonstrated in diverse organisms, report evaluating the RNA chaperone and protein chaperone activity of a given protein is severely limited. Here, two glycine-rich RNA-binding proteins in Arabidopsis thaliana (AtGRPs), AtGRP7 exhibiting RNA chaperone activity and AtGRP4 exhibiting no RNA chaperone activity, were investigated for their protein chaperone activity. The heat-induced thermal aggregation of a substrate protein was significantly decreased with the addition of AtGRP4 depending on protein concentration, whereas the thermal aggregation of a substrate protein was further increased with the addition of AtGRP7, demonstrating that AtGRP4 but not AtGRP7 possesses protein chaperone activity. Size exclusion chromatography and electron microscopy analyses revealed that the formation of high molecular weight (HMW) complexes is closely related to the protein chaperone activity of AtGRP4. Importantly, the additional 25 amino acids at the N-terminus of AtGRP4 are crucial for HMW complex formation and protein chaperone activity. Taken together, these results show that the formation of HMW complexes is important for determining the RNA chaperone and protein chaperone activity of AtGRP4 and AtGRP7.
authors.affiliation: Department of Plant Biotechnology, College of Agriculture and Life Sciences, Chonnam National University, 300 Yongbong-dong, Buk-gu, Kwangju, 500-757, Korea.
authors.forename: Ji Hoon
authors.initials: JH
authors.lastname: Han
authors.forename: Young Jun
authors.initials: YJ
authors.lastname: Jung
authors.forename: Hyun-Ju
authors.initials: HJ
authors.lastname: Lee
authors.forename: Hyun Suk
authors.initials: HS
authors.lastname: Jung
authors.forename: Kyun Oh
authors.initials: KO
authors.lastname: Lee
authors.forename: Hunseung
authors.initials: H
authors.lastname: Kang
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D018832:Molecular Chaperones / Q000737:chemistry / Q000378:metabolism*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D011485:Protein Binding; D017510:Protein Folding; D018749:RNA, Plant / Q000737:chemistry* / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; D018832:Molecular Chaperones; D018749:RNA, Plant; D016601:RNA-Binding Proteins; C118496:GRP7 protein, Arabidopsis; C506809:glycine-rich RNA-binding protein 4, Arabidopsis
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