title: Spatial distribution of D1R- and D2R-expressing medium-sized spiny neurons differs along the rostro-caudal axis of the mouse dorsal striatum.
abstract: The striatum projection neurons are striatonigral and striatopallidal medium-sized spiny neurons (MSNs) that preferentially express D1 (D1R) and D2 (D2R) dopamine receptors, respectively. It is generally assumed that these neurons are physically intermingled, without cytoarchitectural organization although this has not been tested. To address this question we used BAC transgenic mice expressing enhanced green fluorescence (EGFP) under the control of Drd1a or Drd2 promoter and spatial point pattern statistics. We demonstrate that D1R- and D2R-expressing MSNs are randomly distributed in most of the dorsal striatum, whereas a specific region in the caudal striatum, adjacent to the GPe, lacks neurons expressing markers for indirect pathway neurons. This area comprises almost exclusively D1R-expressing MSNs. These neurons receive excitatory inputs from the primary auditory cortex and the medial geniculate thalamic nucleus and a rich dopamine innervation. This area contains cholinergic and GABAergic interneurons but apparently no D2R/A2aR modulation because no fluorescence was detected in the neuropil of Drd2-EGFP or Drd2-Cre, and Adora-Cre BAC transgenic mice crossed with reporter mice. This striatal area that expresses calbindin D28k, VGluT1 and 2, is poor in μ opiate receptors and preproenkephalin. Altogether, the differences observed in D1R-MSNs, D2R-MSNs, and interneurons densities, as well as the anatomical segregation of D1R- and D2R/A2aR-expressing MSNs suggest that there are regional differences in the organization of the striatum.
authors.affiliation: CNRS, UMR 5203, Institut de Génomique Fonctionnelle Montpellier, France ; INSERM, U661 Montpellier, France ; Universités de Montpellier 1 & 2, UMR 5203 Montpellier, France.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Gangarossa
authors.forename: Julie
authors.initials: J
authors.lastname: Espallergues
authors.forename: Philippe
authors.initials: P
authors.lastname: Mailly
authors.forename: Dimitri
authors.initials: D
authors.lastname: De Bundel
authors.forename: Alban
authors.initials: A
authors.lastname: de Kerchove d'Exaerde
authors.forename: Denis
authors.initials: D
authors.lastname: Hervé
authors.forename: Jean-Antoine
authors.initials: JA
authors.lastname: Girault
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Valjent
authors.forename: Patrik
authors.initials: P
authors.lastname: Krieger
mesh_terms: D000818:Animals; D003342:Corpus Striatum / Q000166:cytology / Q000378:metabolism*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D009474:Neurons / Q000378:metabolism*; D011897:Random Allocation; D017447:Receptors, Dopamine D1 / Q000096:biosynthesis*; D017448:Receptors, Dopamine D2 / Q000096:biosynthesis*
chemical_list: D017447:Receptors, Dopamine D1; D017448:Receptors, Dopamine D2
keywords: BAC transgenic mice; adenosine A2a receptor; dopamine D1 and D2 receptors; medium-sized spiny neurons
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