title: Blood protein adsorption on sulfonated chitosan and κ-carrageenan films.
abstract: Many strategies have been reported to improve compatibility of biopolymers using chemical and physical modifications. One possibility is the introduction of sulfonate groups (R-SO3(-)) in the chitosan chain. Another biopolymer with similar characteristics to those of heparin is κ-carrageenan. This study proposed to investigate the application of these two polymers, based on their potential for globular protein adsorption (BSA and fibrinogen). Polymeric films of chitosan and κ-carrageenan were prepared; all films were characterized by elemental analyses, FTIR, XPS and SEM. Characterization techniques showed that the chitosan chain was modified and confirmed the existence of sulfonate groups, as well as in the κ-carrageenan chain, indicating surfaces with similar chemical properties to those of heparin. The effect of charge density was observed for each adsorption condition (BSA at pH 5.0 and 7.4). A more pronounced adsorption rate was observed at pH 5.0 than at pH 7.4 and equilibrium adsorption was achieved, in both cases, after approximately 20 min. The equilibrium data indicate a lower adsorption rate for the sulfonated chitosan film, in comparison to the other films. These results confirm the potential of modified chitosan for use in applications in which globular protein adsorption should be avoided.
authors.affiliation: Grupo de Pesquisas em Separações por Adsorção, Department of Chemical Engineering, Universidade Federal do Ceará, CEP 60455-760 Fortaleza, CE, Brazil.
authors.forename: Pedro H L
authors.initials: PH
authors.lastname: Lima
authors.affiliation: Grupo de Pesquisas em Separações por Adsorção, Department of Chemical Engineering, Universidade Federal do Ceará, CEP 60455-760 Fortaleza, CE, Brazil.
authors.forename: Saulo V A
authors.initials: SV
authors.lastname: Pereira
authors.affiliation: Departamento de Termofluidodinâmica, Faculdade de Engenharia Química, Universidade Estadual de Campinas, Caixa Postal 6066, CEP 13081-970 Campinas, SP, Brazil.
authors.forename: Rodrigo B
authors.initials: RB
authors.lastname: Rabello
authors.affiliation: Departamento de Química Inorgánica, Facultad de Ciencias, Universidad de Málaga, 29071 Málaga, Spain.
authors.forename: Enrique
authors.initials: E
authors.lastname: Rodriguez-Castellón
authors.affiliation: Departamento de Termofluidodinâmica, Faculdade de Engenharia Química, Universidade Estadual de Campinas, Caixa Postal 6066, CEP 13081-970 Campinas, SP, Brazil.
authors.forename: Marisa M
authors.initials: MM
authors.lastname: Beppu
authors.affiliation: Laboratory for Biomaterials and Bioengineering, Canada Research Chair I in Biomaterials and Bioengineering for the Innovation in Surgery, Department of Min-Met-Materials Engineering, Laval University & University Hospital Research Center, Quebec City G1V 0A6, Canada.
authors.forename: Pascale
authors.initials: P
authors.lastname: Chevallier
authors.affiliation: Laboratory for Biomaterials and Bioengineering, Canada Research Chair I in Biomaterials and Bioengineering for the Innovation in Surgery, Department of Min-Met-Materials Engineering, Laval University & University Hospital Research Center, Quebec City G1V 0A6, Canada.
authors.forename: Diego
authors.initials: D
authors.lastname: Mantovani
authors.affiliation: Grupo de Pesquisas em Separações por Adsorção, Department of Chemical Engineering, Universidade Federal do Ceará, CEP 60455-760 Fortaleza, CE, Brazil. Electronic address: rodrigo@gpsa.ufc.br.
authors.forename: Rodrigo S
authors.initials: RS
authors.lastname: Vieira
mesh_terms: D000327:Adsorption; D000818:Animals; D002351:Carrageenan / Q000737:chemistry*; D002417:Cattle; D048271:Chitosan / Q000737:chemistry*; D005340:Fibrinogen / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D018625:Microscopy, Atomic Force; D008855:Microscopy, Electron, Scanning; D012710:Serum Albumin, Bovine / Q000378:metabolism*; D017550:Spectroscopy, Fourier Transform Infrared; D013451:Sulfonic Acids / Q000737:chemistry*
chemical_list: D013451:Sulfonic Acids; D012710:Serum Albumin, Bovine; D002351:Carrageenan; D005340:Fibrinogen; D048271:Chitosan
keywords: Antithrombogenic; Blood device; Carrageenan; Chitosan; Protein adsorption