title: Lgr4 is a key regulator of prostate development and prostate stem cell differentiation.
abstract: Mechanisms modulating prostate cell fate determination remain unexplored. The leucine-rich repeat containing G-protein-coupled receptors (Lgr) have been identified as important stem cell markers in various tissues. Here, we investigated the roles of Lgr4/Gpr48 in prostate stem cells (PSCs) and development. Lgr4 was ubiquitously expressed during early prostate development prior to lineage specification, with adult expression restricted to a few basal cells (principally Lin(-)Sca1(+)CD49f(+)). Lgr4(-/-) mice had compromised branching morphogenesis and delayed epithelial differentiation, leading to decreased prostate size and impaired luminal cell function. In vitro prostate sphere culture revealed that Lgr4(-/-) Lin(-)/Sca1(+)/CD49f(+) cells failed to generate p63(low) cells, indicating a differentiation deficiency. Furthermore, Lgr4 ablation arrested PSC differentiation of in vivo kidney capsule prostate grafts, suggesting that Lgr4 modulates PSC properties independent of hormonal and mesenchymal effects. Analysis of neonatal prostates and prostate spheres revealed a decrease in Wnt, Sonic Hedgehog, and Notch1 expression in Lgr4(-/-) cells. Lgr4 loss blocked differentiation of prostate sphere p63(hi) cells to p63(low). Treatment with exogenous Sonic Hedgehog partially restored the differentiation of p63(hi) cells in Lgr4(-/-) spheres. Taken together, our data revealed the roles of Lgr4 in early prostate development and in stem cell differentiation through regulation of the Wnt, Notch, and Sonic Hedgehog signaling pathways.
authors.affiliation: Center for Cancer and Stem Cell Biology, Institute of Biosciences and Technology, TexasA&M University Health Science Center, Houston, Texas, USA.
authors.forename: Weijia
authors.initials: W
authors.lastname: Luo
authors.forename: Melissa
authors.initials: M
authors.lastname: Rodriguez
authors.forename: Joseph M
authors.initials: JM
authors.lastname: Valdez
authors.forename: Xinglei
authors.initials: X
authors.lastname: Zhu
authors.forename: Kunrong
authors.initials: K
authors.lastname: Tan
authors.forename: Dali
authors.initials: D
authors.lastname: Li
authors.forename: Stefan
authors.initials: S
authors.lastname: Siwko
authors.forename: Li
authors.initials: L
authors.lastname: Xin
authors.forename: Mingyao
authors.initials: M
authors.lastname: Liu
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000502:physiology; D004195:Disease Models, Animal; D006801:Humans; D008297:Male; D051379:Mice; D011467:Prostate / Q000166:cytology / Q000254:growth & development* / Q000378:metabolism; D043562:Receptors, G-Protein-Coupled / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction
chemical_list: C496200:LGR4 protein, mouse; D043562:Receptors, G-Protein-Coupled
keywords: Gpr48; Lgr4; Prostate development; Prostate stem cells
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