title: The effect of laminar air flow and door openings on operating room contamination.
abstract: We evaluate the association of laminar airflow (LAF) and OR traffic with intraoperative contamination rates. Two sterile basins were placed in each room during 81 cases, one inside and one outside the LAF. One Replicate Organism Detection and Counting (RODAC) plate from each basin was sent for culture at successive 30-minute intervals from incision time until wound closure. At successive 30-minute intervals more plates were contaminated outside than inside the LAF. A negative binomial model showed that the bacteria colony forming units (CFU) depended on whether there were any door openings (P=0.02) and the presence of LAF (P=0.003). LAF decreases CFU by 36.6%. LAF independently reduces the risk of contamination and microbial counts for surgeries lasting 90 minutes or less.
authors.affiliation: Orthopaedic Surgery at the Rothman Institute, Bryn Mawr Hospital, Bryn Mawr, Pennsylvania.
authors.forename: Eric B
authors.initials: EB
authors.lastname: Smith
authors.forename: Ibrahim J
authors.initials: IJ
authors.lastname: Raphael
authors.forename: Mitchell G
authors.initials: MG
authors.lastname: Maltenfort
authors.forename: Sittisak
authors.initials: S
authors.lastname: Honsawek
authors.forename: Kyle
authors.initials: K
authors.lastname: Dolan
authors.forename: Elizabeth A
authors.initials: EA
authors.lastname: Younkins
mesh_terms: D004780:Environment, Controlled; D004866:Equipment Contamination / Q000517:prevention & control*; D006801:Humans; D017053:Infection Control / Q000379:methods*; D008828:Microbiological Techniques; D009873:Operating Rooms / Q000458:organization & administration / Q000592:standards*; D019637:Orthopedic Procedures / Q000592:standards*; D011446:Prospective Studies; D014691:Ventilation
keywords: OR traffic; RODAC; arthroplasty; contamination; laminar airflow