title: Optimization of a bioactive exopolysaccharide production from endophytic Fusarium solani SD5.
abstract: Endophytic fungi were less investigated for exopolysaccharide production. In this study endophytic Fusarium solani SD5 was used for optimization of exopolysaccharide production. One variable at a time method and response surface methodology were employed to explore the optimum medium compositions and fermentation conditions. The organism produced maximum exopolysaccharide after 13.68 days of incubation at 28 °C in potato dextrose broth supplemented with (g%/l) glucose, 9.8; yeast extract, 0.69; KCl, 0.05; KH₂PO₄, 0.05 with medium pH 6.46. Use of 50 ml medium in 250 ml Erlenmeyer flask gives highest exopolysaccharide production. The organism produced more than two times higher exopolysaccharide (2.276 ± 0.032 g/l EPS) at optimized condition compared to pre-optimized condition (0.96 ± 0.021). In vivo toxicity test established nontoxic nature of the EPS (≤400 mg EPS/Kg of body weight). The EPS slightly altered intestinal indigenous bacteria and influenced the growth of beneficial Lactobacillus spp.
authors.affiliation: Microbiology Laboratory, Department of Botany and Forestry, Vidyasagar University, Midnapore 721 102, West Bengal, India.
authors.forename: Subhadip
authors.initials: S
authors.lastname: Mahapatra
authors.forename: Debdulal
authors.initials: D
authors.lastname: Banerjee
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D018533:Biomass; D002244:Carbon / Q000494:pharmacology; D003470:Culture Media / Q000494:pharmacology; D060026:Endophytes / Q000737:chemistry*; D005285:Fermentation / Q000187:drug effects; D005670:Fusarium / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration / Q000187:drug effects; D007422:Intestines / Q000187:drug effects / Q000382:microbiology; D008297:Male; D025282:Mycelium / Q000254:growth & development; D009584:Nitrogen / Q000494:pharmacology; D010100:Oxygen / Q000032:analysis; D010710:Phosphates / Q000494:pharmacology; D011134:Polysaccharides / Q000096:biosynthesis* / Q000494:pharmacology / Q000633:toxicity; D051381:Rats; D017208:Rats, Wistar; D012492:Salts / Q000494:pharmacology; D013696:Temperature; D013997:Time Factors
chemical_list: D003470:Culture Media; D010710:Phosphates; D011134:Polysaccharides; D012492:Salts; D002244:Carbon; D009584:Nitrogen; D010100:Oxygen
keywords: Biomass; Endophytic fungi; Exopolysaccharide; Fusarium solani; Optimization; Submerged fermentation