title: LMO4 modulates proliferation and differentiation of 3T3-L1 preadipocytes.
abstract: Previous microarray analyses revealed that LMO4 is expressed in 3T3-L1 preadipocytes, however, its roles in adipogenesis are unknown. In the present study, using RT-PCR sequencing and quantitative real-time RT-PCR, we confirmed that LMO4 gene is expressed in 3T3-L1 preadipocytes and its expression peaks at the early stage of 3T3-L1 preadipocyte differentiation. Further analyses showed that LMO4 knockdown decreased the proliferation of 3T3-L1 preadipocytes, and attenuated the differentiation of 3T3-L1 preadipocytes, as evidenced by reduced lipid accumulation and down-regulation of PPARγ gene expression. Collectively, our findings indicate that LMO4 is a novel modulator of adipogenesis.
authors.affiliation: College of Animal Science and Technology, Northeast Agricultural University, No. 59 Mucai Street, Xiangfang District, Harbin, Heilongjiang 150030, China. wangning@neau.edu.cn
authors.forename: Ning
authors.initials: N
authors.lastname: Wang
authors.forename: Xichen
authors.initials: X
authors.lastname: Wang
authors.forename: Mingxin
authors.initials: M
authors.lastname: Shi
authors.forename: Hongyan
authors.initials: H
authors.lastname: Shi
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Yan
authors.forename: Hui
authors.initials: H
authors.lastname: Li
authors.forename: Shouzhi
authors.initials: S
authors.lastname: Wang
authors.forename: Yuxiang
authors.initials: Y
authors.lastname: Wang
mesh_terms: D041721:3T3-L1 Cells; D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics* / Q000378:metabolism; D017667:Adipocytes / Q000166:cytology / Q000378:metabolism*; D050156:Adipogenesis / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D002454:Cell Differentiation; D049109:Cell Proliferation; D005786:Gene Expression Regulation; D060588:LIM Domain Proteins / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D047495:PPAR gamma / Q000235:genetics* / Q000378:metabolism; D015398:Signal Transduction
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D060588:LIM Domain Proteins; C491976:Lmo4 protein, mouse; D047495:PPAR gamma
keywords: 3T3-L1 preadipocyte; Cell proliferation; LMO4; Preadipocyte differentiation