title: [Genomic characteristics of coxsackievirus B1 MSH/KM9/2009 strain isolated in Yunnan, China].
abstract: To characterize the complete genome sequence of coxsackievirus B1 (CVB1) MSH/KM9/2009 strain isolated from Yunnan, China,2009. Eight overlapping clones covering the whole viral genome (excluding the poly-A tail) were obtained by RT-PCR and sequenced, and their nucleotide and amino acid sequences were compared with other known CVB1 strains. The genome of the CVB1 MSH/KM9/2009 strain had 7384 nucleotides in length, and contained a 741nt non-translated region (NTR) at the 5' end and a 94nt NTR at the 3' end. The entire open reading frame contained 6 549 nt, encoding a 2 183-aa polyprotein. In the coding region, there was no nucleotide deletion or insertion, but some changes of amino acid were unique. The complete genome sequence alignments showed that the CVB1 isolate MSH/KM9/2009 strain shared the highest nucleotide (80.9%, 81.6%, 80.5% and 80%) and amino acid (95.6%, 95.8%, 96.2% and 95.6%) identities to the CVB1 M16560, pmMC, Tucson B1 and CVB1Nm strain, respectively. Phylogenetic tree analysis showed that the MSH/KM9/2009, CVB1 M16560, pmMC, Tucson B1 and CVB1Nm strain clustered into same group. The newly isolated CVB1 strain MSH/KM9/2009 from Yunnan Province belonged to genotype CVB1.
authors.affiliation: Institute of Medical Biology ,Chinese Academy of Medical Sciences and Peking Union Medical College, Kuming 650118, China. ljsh3300@163.com
authors.forename: Jian-Sheng
authors.initials: JS
authors.lastname: Liu
authors.forename: Cong-Wen
authors.initials: CW
authors.lastname: Shao
authors.forename: Yue
authors.initials: Y
authors.lastname: Pan
authors.forename: Yan-Ju
authors.initials: YJ
authors.lastname: Zhu
authors.forename: Xin-Qiang
authors.initials: XQ
authors.lastname: Deng
authors.forename: Hong-Wei
authors.initials: HW
authors.lastname: Liao
authors.forename: Ya-Ling
authors.initials: YL
authors.lastname: Liu
authors.forename: Shao-Hui
authors.initials: SH
authors.lastname: Ma
mesh_terms: D001483:Base Sequence; D002675:Child, Preschool; D002681:China; D003384:Coxsackievirus Infections / Q000821:virology*; D004770:Enterovirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D005260:Female; D016679:Genome, Viral; D005838:Genotype; D006801:Humans; D007223:Infant; D008297:Male; D016366:Open Reading Frames; D010802:Phylogeny; D014764:Viral Proteins / Q000235:genetics
chemical_list: D014764:Viral Proteins