title: In vivo localization in Arabidopsis protoplasts and root tissue.
abstract: In eukaryotic cells, a large number of proteins are transported to their final destination after translation by a process called intracellular trafficking. Transient gene expression, either in plant protoplasts or in specific plant tissues, is a fast, flexible, and reproducible approach to study the cellular function of proteins, protein subcellular localizations, and protein-protein interactions. Here we describe the general method of protoplast isolation, polyethylene glycol-mediated protoplast transformation and immunostaining of protoplast or intact root tissues for studying the localization of protein in Arabidopsis.
authors.affiliation: Division of Integrative Biosciences and Biotechnology, Pohang University of Science and Technology, Pohang, South Korea.
authors.forename: Myoung Hui
authors.initials: MH
authors.lastname: Lee
authors.forename: Yongjik
authors.initials: Y
authors.lastname: Lee
authors.forename: Inhwan
authors.initials: I
authors.lastname: Hwang
mesh_terms: D017360:Arabidopsis / Q000648:ultrastructure; D029681:Arabidopsis Proteins / Q000648:ultrastructure; D008967:Molecular Biology / Q000379:methods*; D018517:Plant Roots / Q000737:chemistry / Q000648:ultrastructure*; D011523:Protoplasts / Q000378:metabolism / Q000648:ultrastructure*
chemical_list: D029681:Arabidopsis Proteins