title: [8]-Gingerol inhibits melanogenesis in murine melanoma cells through down-regulation of the MAPK and PKA signal pathways.
abstract: [8]-Gingerol is an active component of Zinger and shows several pharmacological activities, such as antipyretic and anti-inflammation characteristics. To identify a potential skin-whitening agent, the inhibitory effects of [8]-gingerol on melanogenesis and its mechanism of action were investigated. In the present study, the effects of [8]-gingerol on mushroom tyrosinase, tyrosinase activity and melanin content were determined spectrophotometrically; the expression of melanogenesis-related proteins in B16F10 and B16F1 melanoma cells were determined by Western blotting. Furthermore, the possible signaling pathways involved in [8]-gingerol-mediated depigmentation were also investigated using specific inhibitors. The results revealed that [8]-gingerol (5-100μM) effectively suppressed intracellular tyrosinase activity and decreased the amount of melanin in B16F10 and B16F1 cells. In addition, [8]-gingerol also effectively decreased intracellular reactive species (RS) and reactive oxygen species (ROS) levels at the same dose range. Our results indicated that [8]-gingerol inhibited melanogenesis in B16F10 and B16F1 cells by down-regulation of both mitogen-activated protein kinases (MAPK) and protein kinase A (PKA) signaling pathways or through its antioxidant properties. Hence, [8]-gingerol could be used as an effective skin-whitening agent.
authors.affiliation: Department of Medical Laboratory Science and Biotechnology, China Medical University, No. 91 Hsueh-Shih Road, Taichung 40402, Taiwan, ROC. lchuang@mail.cmu.edu.tw
authors.forename: Huey-Chun
authors.initials: HC
authors.lastname: Huang
authors.forename: Yin-Chun
authors.initials: YC
authors.lastname: Chou
authors.forename: Chia-Yin
authors.initials: CY
authors.lastname: Wu
authors.forename: Tsong-Min
authors.initials: TM
authors.lastname: Chang
mesh_terms: D000818:Animals; D002396:Catechols / Q000494:pharmacology*; D045744:Cell Line, Tumor; D002470:Cell Survival; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism*; D015536:Down-Regulation; D005233:Fatty Alcohols / Q000494:pharmacology*; D015971:Gene Expression Regulation, Enzymologic; D019746:Intramolecular Oxidoreductases / Q000037:antagonists & inhibitors; D020935:MAP Kinase Signaling System; D008543:Melanins / Q000096:biosynthesis*; D008545:Melanoma / Q000378:metabolism*; D008546:Melanoma, Experimental; D051379:Mice; D051739:Microphthalmia-Associated Transcription Factor / Q000037:antagonists & inhibitors; D014442:Monophenol Monooxygenase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D017382:Reactive Oxygen Species; D044102:Receptor, Melanocortin, Type 1 / Q000037:antagonists & inhibitors; D015398:Signal Transduction
chemical_list: D002396:Catechols; D005233:Fatty Alcohols; D008543:Melanins; D051739:Microphthalmia-Associated Transcription Factor; C497235:Mitf protein, mouse; D017382:Reactive Oxygen Species; D044102:Receptor, Melanocortin, Type 1; C007845:gingerol; D014442:Monophenol Monooxygenase; D017868:Cyclic AMP-Dependent Protein Kinases; D019746:Intramolecular Oxidoreductases; C042501:dopachrome isomerase
keywords: Melanin; Melanogenesis; Tyrosinase; [8]-Gingerol