title: Proteasome inhibition leads to altered signaling in the proteome of cisplatin-resistant human ovarian carcinoma cell line.
abstract: To address a precise view into molecular mechanisms of apoptotic signaling pathways after single- or combinatory treatments with specific NF-κB- or proteasome inhibitors and/or cisplatin (CDDP), flow cytometry and western blotting of the cell proteome in human ovarian chemosensitive- and CDDP-resistant cell lines were used. We report here that proteasome inhibition (but not NF-κB inhibition) caused marked alterations in the cell proliferation and cell cycle, as well as in the levels of signaling anti- and pro-apoptotic proteins PARP, NF-κB, IκB-α, Bcl-2, Bax, and lysosome-associated LAMP-1 and ATP-7B molecules in particular proteome fractions. These findings refer to the possibility of regulation of CDDP resistance, inclusive the capacity of lysosomes to export CDDP in certain human ovarian cancer cells by proteasome inhibition.
authors.forename: J
authors.initials: J
authors.lastname: Duraj
authors.forename: M
authors.initials: M
authors.lastname: Pastorek
authors.forename: J
authors.initials: J
authors.lastname: Vitkovska
authors.forename: D
authors.initials: D
authors.lastname: Cholujova
authors.forename: P
authors.initials: P
authors.lastname: Gronesova
authors.forename: L
authors.initials: L
authors.lastname: Hunakova
authors.forename: J
authors.initials: J
authors.lastname: Sedlak
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis; D015153:Blotting, Western; D002453:Cell Cycle; D049109:Cell Proliferation; D002945:Cisplatin / Q000494:pharmacology*; D019008:Drug Resistance, Neoplasm; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D010051:Ovarian Neoplasms / Q000188:drug therapy / Q000378:metabolism* / Q000473:pathology; D046988:Proteasome Endopeptidase Complex / Q000737:chemistry* / Q000378:metabolism; D020543:Proteome / Q000032:analysis* / Q000187:drug effects*; D015398:Signal Transduction; D013347:Subcellular Fractions; D014407:Tumor Cells, Cultured
chemical_list: D000970:Antineoplastic Agents; D020543:Proteome; D046988:Proteasome Endopeptidase Complex; D002945:Cisplatin