title: High-resolution temporal response patterns to influenza vaccine reveal a distinct human plasma cell gene signature.
abstract: To identify sources of inter-subject variation in vaccine responses, we performed high-frequency sampling of human peripheral blood cells post-vaccination, followed by a novel systems biology analysis. Functional principal component analysis was used to examine time varying B cell vaccine responses. In subjects vaccinated within the previous three years, 90% of transcriptome variation was explained by a single subject-specific mathematical pattern. Within individual vaccine response patterns, a common subset of 742 genes was strongly correlated with migrating plasma cells. Of these, 366 genes were associated with human plasmablasts differentiating in vitro. Additionally, subject-specific temporal transcriptome patterns in peripheral blood mononuclear cells identified migration of myeloid/dendritic cell lineage cells one day after vaccination. Upstream analyses of transcriptome changes suggested both shared and subject-specific transcription groups underlying larger patterns. With robust statistical methods, time-varying response characteristics of individual subjects were effectively captured along with a shared plasma cell gene signature.
authors.affiliation: Department of Medicine, Division of Nephrology, University of Rochester School of Medicine and Dentistry, Rochester, New York, USA.
authors.forename: Alicia D
authors.initials: AD
authors.lastname: Henn
authors.forename: Shuang
authors.initials: S
authors.lastname: Wu
authors.forename: Xing
authors.initials: X
authors.lastname: Qiu
authors.forename: Melissa
authors.initials: M
authors.lastname: Ruda
authors.forename: Michael
authors.initials: M
authors.lastname: Stover
authors.forename: Hongmei
authors.initials: H
authors.lastname: Yang
authors.forename: Zhiping
authors.initials: Z
authors.lastname: Liu
authors.forename: Stephen L
authors.initials: SL
authors.lastname: Welle
authors.forename: Jeanne
authors.initials: J
authors.lastname: Holden-Wiltse
authors.forename: Hulin
authors.initials: H
authors.lastname: Wu
authors.forename: Martin S
authors.initials: MS
authors.lastname: Zand
mesh_terms: D001402:B-Lymphocytes / Q000187:drug effects* / Q000276:immunology*; D001798:Blood Proteins / Q000235:genetics / Q000276:immunology; D002478:Cells, Cultured; D005786:Gene Expression Regulation / Q000187:drug effects / Q000235:genetics* / Q000276:immunology*; D006801:Humans; D007252:Influenza Vaccines / Q000008:administration & dosage* / Q000276:immunology*; D059467:Transcriptome / Q000187:drug effects / Q000276:immunology*
chemical_list: D001798:Blood Proteins; D007252:Influenza Vaccines
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