title: Enhancement of electrochemical differentiation ability of nucleobases in phosphate buffer solution at pH 7.4.
abstract: The electrochemical behavior of nucleobases has been studied in 0.1 M phosphate buffer solution at pH 7.4, using a bare graphite electrode. Guanine and adenine produced well-defined oxidation peaks at about +0.63 and +0.91 V at 100 mV/s, respectively. Nucleobases exhibit an irreversible and hybrid-controlled electrochemical process, including adsorption and diffusion. The nucleobase oxidation peaks shift due to the selective interactions of nucleobases with each other. The oxidation peaks for three different pyrimidine bases, uracil, cytosine, and thymine, can be clearly identified at +1.26, +1.41, and +1.32 V, respectively. These differences in the electrochemical behavior among nucleobases can be attributed to their different chemical structures.
authors.affiliation: State Key Laboratory of Crystal Materials, Center of Bio & Micro/Nano Functional Materials, Shandong University, Jinan, PR China.
authors.forename: Jingjie
authors.initials: J
authors.lastname: Cui
authors.forename: Dehui
authors.initials: D
authors.lastname: Sun
authors.forename: Xiaoshan
authors.initials: X
authors.lastname: He
authors.forename: Guancong
authors.initials: G
authors.lastname: Wang
authors.forename: Hongshi
authors.initials: H
authors.lastname: Zhao
authors.forename: Hong
authors.initials: H
authors.lastname: Liu
authors.forename: Chenguo
authors.initials: C
authors.lastname: Hu
authors.forename: Zhen
authors.initials: Z
authors.lastname: Huang
authors.forename: Robert I
authors.initials: RI
authors.lastname: Boughton
authors.forename: Huaidong
authors.initials: H
authors.lastname: Jiang
mesh_terms: D004563:Electrochemistry; D006863:Hydrogen-Ion Concentration; D011687:Purines / Q000737:chemistry*; D011743:Pyrimidines / Q000737:chemistry*
chemical_list: D011687:Purines; D011743:Pyrimidines