title: Quantitative analysis of the major constituents in Chinese medicinal preparation SuoQuan formulae by ultra fast high performance liquid chromatography/quadrupole tandem mass spectrometry.
abstract: BACKGROUND
  The SuoQuan formulae containing Fructus Alpiniae Oxyphyllae has been used to combat the urinary incontinence symptoms including frequency, urgency and nocturia for hundreds of years in China. However, the chemical information was not well characterized. The quality control marker constituent only focused on one single compound in the current Chinese Pharmacopeia. Hence it is prudent to identify and quantify the main constituents in this herbal product. This study aimed to analyze the main constituents using ultra-fast performance liquid chromatography coupled to tandem mass spectrometry (UFLC-MS/MS).
  
  
  RESULTS
  Fourteen phytochemicals originated from five chemical classes constituents were identified by comparing the molecular mass, fragmentation pattern and retention time with those of the reference standards. A newly developed UFLC-MS/MS was validated demonstrating that the new assay was valid, reproducible and reliable. This method was successfully applied to simultaneously quantify the fourteen phytochemicals. Notably, the content of these constituents showed significant differences in three pharmaceutical preparations. The major constituent originated from each of chemical class was isolinderalactone, norisoboldine, nootkatone, yakuchinone A and apigenin-4',7-dimethylther, respectively. The variation among these compounds was more than 1000 times. Furthermore, the significant content variation between the two different Suoquan pills was also observed.
  
  
  CONCLUSIONS
  The proposed method is sensitive and reliable; hence it can be used to analyze a variety of SuoQuan formulae products produced by different pharmaceutical manufacturers.
authors.affiliation: School of Pharmacy, Hainan Medical University, Hainan Provincial Key Laboratory of R&D of Tropical Herbs, Haikou 571101, China. cy.chen508@gmail.com.
authors.forename: Feng
authors.initials: F
authors.lastname: Chen
authors.forename: Hai-Long
authors.initials: HL
authors.lastname: Li
authors.forename: Yong-Hui
authors.initials: YH
authors.lastname: Li
authors.forename: Yin-Feng
authors.initials: YF
authors.lastname: Tan
authors.forename: Jun-Qing
authors.initials: JQ
authors.lastname: Zhang
references.citation: J Sep Sci. 2010 Sep;33(17-18):2734-42
references.pmid: 20730831
references.citation: Nature. 2007 Jul 12;448(7150):126-8
references.pmid: 17625539
references.citation: Drug Test Anal. 2014 Mar;6(3):239-45
references.pmid: 23408659
references.citation: J Ethnopharmacol. 2010 Nov 11;132(2):443-9
references.pmid: 20723592
references.citation: J Environ Pathol Toxicol Oncol. 2002;21(2):131-9
references.pmid: 12086399
references.citation: N Engl J Med. 2008 Mar 6;358(10):1029-36
references.pmid: 18322284
references.citation: Inflammation. 2010 Dec;33(6):389-97
references.pmid: 20352482
references.citation: J Cell Biochem. 2012 Aug;113(8):2785-95
references.pmid: 22473817
references.citation: Lancet. 2006 Jan 7;367(9504):57-67
references.pmid: 16399154
references.citation: J Ethnopharmacol. 2007 May 4;111(2):322-8
references.pmid: 17204385
references.citation: J Chromatogr A. 2008 Nov 28;1212(1-2):76-81
references.pmid: 18951552
references.citation: Arch Pharm Res. 2012 Dec;35(12):2143-6
references.pmid: 23263808
references.citation: Oncol Res. 2002;13(1):37-45
references.pmid: 12201673
references.citation: Iran Biomed J. 2011;15(4):134-42
references.pmid: 22395138
references.citation: Eur Urol. 2011 Mar;59(3):387-400
references.pmid: 21130559
references.citation: Acta Pharmacol Sin. 2013 Mar;34(3):403-13
references.pmid: 23396374
references.citation: Cell Mol Neurobiol. 2012 Jan;32(1):27-40
references.pmid: 21744117
references.citation: J Sep Sci. 2010 Apr;33(8):1072-8
references.pmid: 20187034
references.citation: J Nat Prod. 2011 Dec 27;74(12):2489-96
references.pmid: 22148193