title: Effect of laminin 332 on motility and invasion in bladder cancer.
abstract: We examined the correlation between laminin 332 and malignancy in bladder cancer patients, and, using a strain of invasive bladder cancer cells, determined whether laminin 332 causes bladder cancer motility and invasion. To investigate the correlation between laminin 332 g2 distribution and patient outcome, we performed a semiquantitative immunohistochemical analysis of 35 paraffin-embedded samples using the antibody D4B5, which is specific for the laminin 5 γ2 chain. To evaluate the role of laminin 332 in NBT-II cell motility and invasion, we used a scratch assay and the Boyden chamber chemoinvasion system. Tumor stage and grade were significantly correlated with a loss of laminin 332 γ2 chain from the basement membrane (p = 0.001) and its retention in the cytoplasm (p = 0.001) (Kruskal-Wallis test). Kaplan-Meier survival curves revealed an association between the risk of progression and cytoplasmic retention of the laminin 332 γ2 chain. In addition, an in vitro scratch assay showed an increase in the migration of cells treated with laminin 332 from their cluster. The Boyden chamber assay showed that laminin 332 potentiated NBT-II cell invasion. Immunohistochemistry results showed that bladder cancer patients with a higher malignancy expressed more laminin 332. The in vitro scratch and invasion assay showed that laminin 332 stimulated the motility and invasion of bladder cancer cells. The invasion assay explains the correlation between laminin 332 expression and bladder cancer malignancy.
authors.affiliation: Department of Urology, Korea University School of Medicine, Seoul, South Korea.
authors.forename: Sung-Gu
authors.initials: SG
authors.lastname: Kang
authors.forename: Young-Ran
authors.initials: YR
authors.lastname: Ha
authors.forename: Young-Hwii
authors.initials: YH
authors.lastname: Ko
authors.forename: Seok-Ho
authors.initials: SH
authors.lastname: Kang
authors.forename: Kwan-Joong
authors.initials: KJ
authors.lastname: Joo
authors.forename: Hyun-Yee
authors.initials: HY
authors.lastname: Cho
authors.forename: Hong-Seok
authors.initials: HS
authors.lastname: Park
authors.forename: Chul-Hwan
authors.initials: CH
authors.lastname: Kim
authors.forename: Soon-Young
authors.initials: SY
authors.lastname: Kwon
authors.forename: Je-Jong
authors.initials: JJ
authors.lastname: Kim
authors.forename: Jun
authors.initials: J
authors.lastname: Cheon
authors.forename: Jeong-Gu
authors.initials: JG
authors.lastname: Lee
mesh_terms: D000368:Aged; D000818:Animals; D002277:Carcinoma / Q000188:drug therapy* / Q000401:mortality / Q000473:pathology*; D015815:Cell Adhesion Molecules / Q000494:pharmacology*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects*; D005260:Female; D006801:Humans; D053208:Kaplan-Meier Estimate; D008297:Male; D009361:Neoplasm Invasiveness; D011379:Prognosis; D051381:Rats; D012307:Risk Factors; D001749:Urinary Bladder Neoplasms / Q000188:drug therapy* / Q000401:mortality / Q000473:pathology*; D019459:Urothelium / Q000187:drug effects / Q000473:pathology; D000097794:Kalinin
chemical_list: D015815:Cell Adhesion Molecules; D000097794:Kalinin
keywords: Invasion; Laminin 332; Prognosis; Urothelial cell carcinoma
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