title: Preliminary toxicological assessment of phthalate esters from drinking water consumed in Portugal.
abstract: This paper reports, for the first time, the concentrations of selected phthalates in drinking water consumed in Portugal. The use of bottled water in Portugal has increased in recent years. The main material for bottles is polyethylene terephthalate (PET). Its plasticizer components can contaminate water by leaching, and several scientific studies have evidenced potential health risks of phthalates to humans of all ages. With water being one of the most essential elements to human health and because it is consumed by ingestion, the evaluation of drinking water quality, with respect to phthalate contents, is important. This study tested seven commercial brands of bottled water consumed in Portugal, six PET and one glass (the most consumed) bottled water. Furthermore, tap water from Lisbon and three small neighbor cities was analyzed. Phthalates (di-n-butyl phthalate ester (DnBP), bis(2-ethylhexyl) phthalate ester (DEHP), and di-i-butyl phthalate ester (DIBP)) in water samples were quantified (PET and glass) by means of direct immersion solid-phase microextraction and ionic liquid gas chromatography associated with flame ionization detection or mass spectrometry due to their high boiling points and water solubility. The method utilized in this study showed a linear range for target phthalates between 0.02 and 6.5 μg L(-1), good precision and low limits of detection that were between 0.01 and 0.06 μg L(-1), and quantitation between 0.04 and 0.19 μg L(-1). Only three phthalates were detected in Portuguese drinking waters: dibutyl (DnBP), diisobutyl (DIBP), and di(ethylhexyl) phthalate (DEHP). Concentrations ranged between 0.06 and 6.5 μg L(-1) for DnBP, between 0.02 and 0.16 μg L(-1) for DEHP, and between 0.1 and 1.89 μg L(-1) for DIBP. The concentration of DEHP was found to be up to five times higher in PET than in glass bottled water. Surprisingly, all the three phthalates were detected in glass bottled water with the amount of DnBP being higher (6.5 μg L(-1)) than in PET bottled water. These concentrations do not represent direct risk to human health. Regarding potable tap water, only DIBP and DEHP were detected. Two of the cities showed concentration of all three phthalates in their water below the limits of detection of the method. All the samples showed phthalate concentrations below 6 μg L(-1), the maximum admissible concentration in water established by the US Environmental Protection Agency. The concentrations measured in Portuguese bottled waters do not represent any risk for adult's health.
authors.affiliation: Departamento de Química, CQFB-Requimte, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, Quinta da Torre, Caparica, 2825-114, Lisbon, Portugal.
authors.forename: José
authors.initials: J
authors.lastname: Santana
authors.forename: Clara
authors.initials: C
authors.lastname: Giraudi
authors.forename: Emílio
authors.initials: E
authors.lastname: Marengo
authors.forename: Elisa
authors.initials: E
authors.lastname: Robotti
authors.forename: Sara
authors.initials: S
authors.lastname: Pires
authors.forename: Inês
authors.initials: I
authors.lastname: Nunes
authors.forename: Elvira M
authors.initials: EM
authors.lastname: Gaspar
mesh_terms: D000328:Adult; D003993:Dibutyl Phthalate / Q000032:analysis / Q000633:toxicity; D004032:Diet / Q000706:statistics & numerical data; D004051:Diethylhexyl Phthalate / Q000032:analysis / Q000633:toxicity; D060766:Drinking Water / Q000737:chemistry*; D004781:Environmental Exposure / Q000032:analysis / Q000706:statistics & numerical data; D005506:Food Contamination / Q000032:analysis / Q000706:statistics & numerical data*; D006801:Humans; D010795:Phthalic Acids / Q000032:analysis / Q000633:toxicity*; D010968:Plasticizers / Q000032:analysis / Q000633:toxicity; D011093:Polyethylene Terephthalates / Q000737:chemistry; D011174:Portugal; D052617:Solid Phase Microextraction
chemical_list: D060766:Drinking Water; D010795:Phthalic Acids; D010968:Plasticizers; D011093:Polyethylene Terephthalates; D003993:Dibutyl Phthalate; C032279:phthalic acid; D004051:Diethylhexyl Phthalate
references.citation: Environ Int. 2012 Jul;42:78-83
references.pmid: 21524797
references.citation: J Chromatogr A. 2008 Jan 18;1178(1-2):231-8
references.pmid: 18082753
references.citation: Environ Health Perspect. 2010 Apr;118(4):445-8
references.pmid: 20368129
references.citation: J Chromatogr B Analyt Technol Biomed Life Sci. 2005 Feb 25;816(1-2):269-80
references.pmid: 15664359
references.citation: Anal Chim Acta. 2010 Jun 11;669(1-2):32-8
references.pmid: 20510900
references.citation: Int J Hyg Environ Health. 2011 Nov;214(6):442-8
references.pmid: 21885335
references.citation: J Chromatogr A. 2012 Sep 14;1255:130-44
references.pmid: 22621883
references.citation: J Chromatogr A. 2001 Jul 13;922(1-2):377-84
references.pmid: 11486887
references.citation: J Chromatogr A. 2005 Apr 22;1072(1):63-72
references.pmid: 15881460
references.citation: J Chromatogr A. 2000 Mar 3;872(1-2):191-201
references.pmid: 10749497
references.citation: J Steroid Biochem Mol Biol. 2011 Nov;127(3-5):204-15
references.pmid: 21899826
references.citation: J Toxicol Environ Health B Crit Rev. 2009 Feb;12(2):157-74
references.pmid: 19235623
references.citation: Water Res. 2008 Dec;42(20):5054-60
references.pmid: 18929387
references.citation: Environ Health Perspect. 1982 Nov;45:129-33
references.pmid: 7140685
references.citation: J Chromatogr A. 2002 Nov 29;978(1-2):165-75
references.pmid: 12458953
references.citation: Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2008 Apr;25(4):511-8
references.pmid: 18348049