title: Inhibition of initial adhesion of oral bacteria through a lectin from Bauhinia variegata L. var. variegata expressed in Escherichia coli.
abstract: OBJECTIVE
  The aim of the present work was to study the in vitro effect of native and recombinant Bauhinia variegata var. variegata lectins in inhibiting early adhesion of Streptococcus mutans, Streptococcus sanguis and Streptococcus sobrinus to experimentally acquired pellicle.
  
  
  RESULTS
  Native lectin from B. variegata (BVL) was purified by affinity chromatography of extract of seeds. The recombinant lectin (rBVL-I) was expressed in E. coli strain BL21 (DE3) from a genomic clone encoding the mature B. variegata lectin gene using the vector pAE-bvlI. Recombinant protein deposited in inclusion bodies was solubilized and subsequently purified by affinity chromatography. The rBVL-I was compared to BVL for agglutination of erythrocytes and initial adherence of oral bacteria on a saliva-coated surface. The results revealed that rBVL-I acts similarly to BVL for agglutination of erythrocytes. Both lectins showed adhesion inhibition effect on Step. sanguis, Step. mutans and Step. sobrinus.
  
  
  CONCLUSIONS
  We report, for the first time, the inhibition of early adhesion of oral bacteria by a recombinant lectin.
  
  
  CONCLUSIONS
  Our results support the proposed biotechnological application of lectins in a strategy to reduce development of dental caries by inhibiting the initial adhesion and biofilm formation.
authors.affiliation: Núcleo Biotecnologia, Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas, Pelotas, Brazil; Faculdade de Medicina, Laboratório de Micologia, Universidade Federal de Rio Grande, Rio Grande, Brazil.
authors.forename: G B
authors.initials: GB
authors.lastname: Klafke
authors.forename: S
authors.initials: S
authors.lastname: Borsuk
authors.forename: R A
authors.initials: RA
authors.lastname: Gonçales
authors.forename: F V S
authors.initials: FV
authors.lastname: Arruda
authors.forename: V A
authors.initials: VA
authors.lastname: Carneiro
authors.forename: E H
authors.initials: EH
authors.lastname: Teixeira
authors.forename: A L
authors.initials: AL
authors.lastname: Coelho da Silva
authors.forename: B S
authors.initials: BS
authors.lastname: Cavada
authors.forename: O A
authors.initials: OA
authors.lastname: Dellagostin
authors.forename: L S
authors.initials: LS
authors.lastname: Pinto
mesh_terms: D000818:Animals; D001422:Bacterial Adhesion / Q000187:drug effects*; D036481:Bauhinia / Q000737:chemistry*; D018441:Biofilms / Q000187:drug effects; D002846:Chromatography, Affinity; D004926:Escherichia coli / Q000378:metabolism*; D006386:Hemagglutination Tests; D006801:Humans; D037102:Lectins / Q000302:isolation & purification / Q000494:pharmacology*; D010936:Plant Extracts / Q000737:chemistry; D011817:Rabbits; D011994:Recombinant Proteins / Q000494:pharmacology; D012463:Saliva / Q000737:chemistry; D012639:Seeds / Q000737:chemistry; D013291:Streptococcus / Q000187:drug effects* / Q000502:physiology
chemical_list: D037102:Lectins; D010936:Plant Extracts; D011994:Recombinant Proteins
keywords: Streptococcus mutans; Streptococcus sanguis; Streptococcus sobrinus; acquired pellicle; rBVL-I