title: Drug-to-antibody ratio (DAR) and drug load distribution by LC-ESI-MS.
abstract: This chapter describes an LC-ESI-MS method for the DAR and drug load distribution analysis that is suitable for lysine-linked ADCs. The ADC sample is desalted using a reversed-phase LC column with an acetonitrile gradient prior to online MS analysis. The MS spectrum is processed (deconvoluted) and converted to a series of zero charge state masses that corresponds to the increasing number of drugs in the ADC. Integration of the mass peak area allows the calculation of the DAR and drug load distribution of ADCs.
authors.affiliation: Protein Analytical Chemistry, Genentech Inc., South San Francisco, CA, USA.
authors.forename: Louisette
authors.initials: L
authors.lastname: Basa
mesh_terms: D053000:Analytic Sample Preparation Methods; D002853:Chromatography, Liquid / Q000379:methods*; D018796:Immunoconjugates / Q000032:analysis* / Q000737:chemistry*; D004364:Pharmaceutical Preparations / Q000032:analysis* / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
chemical_list: D018796:Immunoconjugates; D004364:Pharmaceutical Preparations