title: Capturing intercellular sugar-mediated ligand-receptor recognitions via a simple yet highly biospecific interfacial system.
abstract: Intercellular ligand-receptor recognitions are crucial natural interactions that initiate a number of biological and pathological events. We present here the simple construction of a unique class of biomimetic interfaces based on a graphene-mediated self-assembly of glycosyl anthraquinones to a screen-printed electrode for the detection of transmembrane glycoprotein receptors expressed on a hepatoma cell line. We show that an electroactive interface confined with densely clustered galactosyl ligands is able to ingeniously recognize the asialoglycoprotein receptors on live Hep-G2 cells employing simple electrochemical techniques. The only facility used is a personal laptop in connection with a cheap and portable electrochemical workstation.
authors.affiliation: State Key Laboratory of Bioreactor Engineering, and Key Laboratory for Advanced Materials & Institute of Fine Chemicals, East China University of Science and Technology, Shanghai 200237, PR China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Li
authors.forename: Si-Si
authors.initials: SS
authors.lastname: Deng
authors.forename: Yi
authors.initials: Y
authors.lastname: Zang
authors.forename: Zhen
authors.initials: Z
authors.lastname: Gu
authors.forename: Xiao-Peng
authors.initials: XP
authors.lastname: He
authors.forename: Guo-Rong
authors.initials: GR
authors.lastname: Chen
authors.forename: Kaixian
authors.initials: K
authors.lastname: Chen
authors.forename: Tony D
authors.initials: TD
authors.lastname: James
authors.forename: Jia
authors.initials: J
authors.lastname: Li
authors.forename: Yi-Tao
authors.initials: YT
authors.lastname: Long
mesh_terms: D000880:Anthraquinones / Q000737:chemistry; D040761:Biomimetic Materials; D032701:Biomimetics; D015374:Biosensing Techniques; D050260:Carbohydrate Metabolism; D002241:Carbohydrates / Q000737:chemistry*; D045744:Cell Line, Tumor; D004563:Electrochemistry; D004566:Electrodes; D006108:Graphite / Q000737:chemistry / Q000378:metabolism; D056945:Hep G2 Cells; D006801:Humans; D007700:Kinetics; D037102:Lectins / Q000737:chemistry / Q000378:metabolism; D008024:Ligands; D018625:Microscopy, Atomic Force; D010087:Oxides / Q000737:chemistry; D011485:Protein Binding; D011956:Receptors, Cell Surface / Q000737:chemistry / Q000378:metabolism; D018160:Receptors, Cytoplasmic and Nuclear / Q000737:chemistry / Q000378:metabolism*
chemical_list: D000880:Anthraquinones; D002241:Carbohydrates; D037102:Lectins; D008024:Ligands; D010087:Oxides; D011956:Receptors, Cell Surface; D018160:Receptors, Cytoplasmic and Nuclear; D006108:Graphite
references.citation: Chem Biol. 2002 Apr;9(4):443-54
references.pmid: 11983333
references.citation: Small. 2010 Mar 22;6(6):711-23
references.pmid: 20225186
references.citation: J Biol Chem. 1996 Aug 30;271(35):21160-6
references.pmid: 8702886
references.citation: J Am Chem Soc. 2008 May 7;130(18):5856-7
references.pmid: 18399634
references.citation: Biosens Bioelectron. 2011 Mar 15;26(7):3181-6
references.pmid: 21255992
references.citation: Angew Chem Int Ed Engl. 2009;48(42):7752-77
references.pmid: 19784976
references.citation: Chem Commun (Camb). 2011 Jun 14;47(22):6201-7
references.pmid: 21503322
references.citation: Cell. 2006 Sep 8;126(5):855-67
references.pmid: 16959566
references.citation: Nature. 2007 Apr 26;446(7139):1017-22
references.pmid: 17460662
references.citation: Science. 2004 Oct 22;306(5696):666-9
references.pmid: 15499015
references.citation: J Org Chem. 2002 May 3;67(9):3057-64
references.pmid: 11975567
references.citation: Science. 2009 Jun 19;324(5934):1530-4
references.pmid: 19541989
references.citation: Curr Opin Struct Biol. 2003 Oct;13(5):637-45
references.pmid: 14568620
references.citation: Anal Chem. 2012 Jan 3;84(1):241-6
references.pmid: 22035288
references.citation: Angew Chem Int Ed Engl. 2002 Jul 15;41(14):2596-9
references.pmid: 12203546
references.citation: J Viral Hepat. 2006 Mar;13(3):158-65
references.pmid: 16475991
references.citation: J Am Chem Soc. 2011 Mar 16;133(10):3649-57
references.pmid: 21341799
references.citation: Chem Rev. 1998 Apr 2;98(2):637-674
references.pmid: 11848911
references.citation: Nat Mater. 2007 Mar;6(3):183-91
references.pmid: 17330084
references.citation: Nature. 2007 Apr 26;446(7139):1030-7
references.pmid: 17460664
references.citation: Science. 2001 Mar 23;291(5512):2357-64
references.pmid: 11269316
references.citation: Nat Rev Drug Discov. 2009 Aug;8(8):661-77
references.pmid: 19629075
references.citation: Angew Chem Int Ed Engl. 2002 Oct 4;41(19):3583-6, 3513
references.pmid: 12370898
references.citation: Nat Nanotechnol. 2009 Apr;4(4):217-24
references.pmid: 19350030
references.citation: Nat Biotechnol. 2002 Mar;20(3):275-81
references.pmid: 11875429
references.citation: Cell. 2010 Nov 24;143(5):672-6
references.pmid: 21111227
references.citation: Nat Chem Biol. 2006 May;2(5):238-48
references.pmid: 16619023
references.citation: Glycobiology. 1993 Apr;3(2):97-130
references.pmid: 8490246
references.citation: Nat Rev Cancer. 2005 Jan;5(1):29-41
references.pmid: 15630413
references.citation: Curr Opin Struct Biol. 2004 Oct;14(5):631-7
references.pmid: 15465325
references.citation: Chem Rev. 2010 Dec 8;110(12):PR1-40
references.pmid: 21192326