title: Native/Denaturing two-dimensional DNA electrophoresis and its application to the analysis of recombination intermediates.
abstract: Two-dimensional (2D) gel electrophoresis employs distinct electrophoretic conditions to better resolve complex mixtures of molecules. In combination with Southern analysis, 2D agarose gel electrophoresis is routinely employed to detect and analyze DNA intermediates that arise during the replication and repair of chromosomes. By separating intermediates into their component single-strands, native/denaturing 2D gels can reveal structure that is not apparent under native conditions alone. Here, we describe a general method for native/denaturing two-dimensional gel electrophoresis and its application to understanding the DNA strand-composition of recombination intermediates formed during meiosis.
authors.affiliation: Howard Hughes Medical Institute and the Departments of Microbiology & Molecular Genetics, Molecular & Cellular Biology and Cell Biology & Human Anatomy, University of California Davis, Davis, CA, USA.
authors.forename: Jessica P
authors.initials: JP
authors.lastname: Lao
authors.forename: Shangming
authors.initials: S
authors.lastname: Tang
authors.forename: Neil
authors.initials: N
authors.lastname: Hunter
mesh_terms: D004247:DNA / Q000737:chemistry*; D004261:DNA Replication; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D009690:Nucleic Acid Conformation; D009691:Nucleic Acid Denaturation; D011995:Recombination, Genetic / Q000235:genetics*
chemical_list: D004247:DNA