title: Protective effect of soybeans as protein source in the diet against cadmium-aorta redox and morphological alteration.
abstract: We investigated the effects of cadmium exposition on thoracic aorta redox status and morphology, and the putative protective effect of soybeans in the diet. Male Wistar rats were separated into 6 groups: 3 fed with a diet containing casein and 3 containing soybeans, as protein source. Within each protein group, one was given tap water (control) and the other two tap water containing 15 and 100 ppm of Cd(2+), respectively, for two months. In rats fed with casein diet, 15 ppm of Cd induced an increase of thiobarbituric acid-reactive substances (TBARS), and of the catalase (CAT) and glutathione peroxidase (GPx) activities, which were even higher with 100 ppm of Cd(2+), in aorta. Also, 100 ppm Cd(2+) exposure increased superoxide dismutase (CuZnSOD) activity; CAT, GPX, SOD, Nrf2 and metallothioneine II mRNA expressions and CAT, GPx and NOX-2 protein levels, compared with control. Aorta endothelial and cytoplasmic alterations were observed. However, with the soybeans diet, 15 and 100 ppm of Cd(2+) did not modify TBARS levels; CAT, GPX and Nrf2 mRNA expressions; CAT, GPx and NOX-2 protein; and the aorta morphology, compared with control. The soybean diet attenuates the redox changes and protects against morphological alterations induced, in a dose-dependent way, by Cd in aorta.
authors.affiliation: Department of Biochemistry and Biological Sciences, Faculty of Chemistry, Biochemistry and Pharmacy, National University of San Luis, Argentina; IMIBIO-San Luis CONICET, Argentina.
authors.forename: Matías F F
authors.initials: MF
authors.lastname: Pérez Díaz
authors.forename: Mariano
authors.initials: M
authors.lastname: Acosta
authors.forename: Fabián H
authors.initials: FH
authors.lastname: Mohamed
authors.forename: Mariana L
authors.initials: ML
authors.lastname: Ferramola
authors.forename: Liliana B
authors.initials: LB
authors.lastname: Oliveros
authors.forename: María S
authors.initials: MS
authors.lastname: Gimenez
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage*; D001013:Aorta, Thoracic / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology*; D002104:Cadmium / Q000633:toxicity*; D019610:Cytoprotection / Q000187:drug effects* / Q000502:physiology*; D004044:Dietary Proteins / Q000008:administration & dosage*; D004305:Dose-Response Relationship, Drug; D008297:Male; D010084:Oxidation-Reduction / Q000187:drug effects; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D013025:Glycine max / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D004044:Dietary Proteins; D002104:Cadmium
keywords: Antioxidant enzymes; Aorta; Cadmium; Casein; Soybeans