title: Chemometrical development and comprehensive validation of a solid phase microextraction/gas chromatography-mass spectrometry methodology for the determination of important free and bound primary aromatics in Greek wines.
abstract: In this work, a solid-phase microextraction/gas chromatography-mass spectrometry (SPME/GC-MS) methodology was developed for the determination of 21 free and glycosidically-bound volatile varietal aroma compounds in wines. Initially, a comparison was made of 5 commercially available SPME fibers for the isolation/preconcentration of the target compounds in the headspace (HS) and direct immersion (DI) modes. The statistical significance of the microextraction variables was evaluated using a 2-level Plackett-Burman experimental design; the most significant variables were further optimized using a modified Simplex procedure. Using the selected conditions, a GC-MS method was fully validated for the quantitative determination of the 21 free primary aroma compounds. The hydrophilic bound precursors were isolated by solid-phase extraction (SPE), enzymatically hydrolyzed to liberate them as the free compounds and further detected by SPME/GC-MS. The method has been successfully applied to the analysis of 20 Greek white wine samples.
authors.affiliation: Athens Wine Institute, Hellenic Agricultural Organisation DEMETER, 1 S. Venizelou Street, Lycovryssi, Athens 141 23, Greece.
authors.forename: Maria
authors.initials: M
authors.lastname: Metafa
authors.forename: Anastasios
authors.initials: A
authors.lastname: Economou
mesh_terms: D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006115:Greece; D057230:Limit of Detection; D012015:Reference Standards; D052617:Solid Phase Microextraction / Q000379:methods*; D014920:Wine / Q000032:analysis*
keywords: Chemometrics; Gas chromatography–mass spectrometry; Primary volatile aroma compounds; Solid-phase microextraction; Validation; Wine