title: Electrophoretic implementation of the solution-depletion method for measuring protein adsorption, adsorption kinetics, and adsorption competition among multiple proteins in solution.
abstract: The venerable solution-depletion method is perhaps the most unambiguous method of measuring solute adsorption from solution to solid particles, requiring neither complex instrumentation nor associated interpretive theory. We describe herein an SDS-gel electrophoresis implementation of the solution--depletion method for measuring protein adsorption and protein-adsorption kinetics. Silanized-glass particles with different surface chemistry/energy and hydrophobic sepharose-based chromatographic media are used as example adsorbents. Electrophoretic separation enables quantification of adsorption competition among multiple proteins in solution for the same adsorbent surface, demonstrated herein by adsorption--competition kinetics from binary solution.
authors.affiliation: Department of Optometry and Vision Science, Seoul National University and Technology, Seoul, South Korea.
authors.forename: Hyeran
authors.initials: H
authors.lastname: Noh
authors.forename: Naris
authors.initials: N
authors.lastname: Barnthip
authors.forename: Purnendu
authors.initials: P
authors.lastname: Parhi
authors.forename: Erwin A
authors.initials: EA
authors.lastname: Vogler
mesh_terms: D000327:Adsorption; D002847:Chromatography, Agarose; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D005898:Glass; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D007700:Kinetics; D008967:Molecular Biology / Q000379:methods; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism; D012709:Serum Albumin / Q000737:chemistry; D012996:Solutions / Q000737:chemistry*; D013499:Surface Properties; D014867:Water / Q000737:chemistry
chemical_list: D011506:Proteins; D012709:Serum Albumin; D012996:Solutions; D014867:Water