title: Antioxidant properties and preliminary evaluation of phytochemical composition of different anatomical parts of amaranth.
abstract: Antioxidant properties of amaranth extracts isolated sequentially by acetone and methanol/water from defatted plant leaves, flowers, stems and seeds were assessed by ABTS(+•), DPPH(•), ORAC and total phenols content (TPC) assays. In addition, antioxidant properties of solid plant material were evaluated by the direct QUENCHER method using the same assays. Leaves and flowers of amaranth as well as their extracts possessed the highest antioxidant activities. Radical scavenging capacity in ABTS(+•) assay for leaves, flowers, stems and seeds evaluated by QUENCHER method were 144.24 ± 2.41, 112.33 ± 7.45, 19.05 ± 1.13 and 21.82 ± 1.06 μmol trolox equivalents in 1 g of dry weight, respectively. On-line HPLC-DPPH(•) assay was used to determine the activity of separated compounds and it was observed that rutin was the main radical scavenger in amaranth extracts. Preliminary screening of extract composition was performed by UPLC/ESI-QTOF-MS and rutin, nicotiflorin, isoquercitrin, 4-hydroxybenzoic and p-coumaric acids were identified by measuring their accurate mass and retention time.
authors.affiliation: Department of Food Technology, Kaunas University of Technology, Kaunas, Lithuania.
authors.forename: Paulius
authors.initials: P
authors.lastname: Kraujalis
authors.forename: Petras R
authors.initials: PR
authors.lastname: Venskutonis
authors.forename: Vaida
authors.initials: V
authors.lastname: Kraujalienė
authors.forename: Audrius
authors.initials: A
authors.lastname: Pukalskas
mesh_terms: D027721:Amaranthus / Q000033:anatomy & histology* / Q000737:chemistry*; D000975:Antioxidants / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D035264:Flowers / Q000737:chemistry; D016166:Free Radical Scavengers; D010636:Phenols / Q000032:analysis; D010936:Plant Extracts / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry; D018547:Plant Stems / Q000737:chemistry; D012431:Rutin / Q000032:analysis; D012639:Seeds; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D000975:Antioxidants; D016166:Free Radical Scavengers; D010636:Phenols; D010936:Plant Extracts; D012431:Rutin
references.citation: J Ethnopharmacol. 2009 Sep 7;125(2):364-6
references.pmid: 19465104
references.citation: Nahrung. 2002 Jun;46(3):184-6
references.pmid: 12108218
references.citation: J Food Sci. 2012 Apr;77(4):R93-104
references.pmid: 22515252
references.citation: Free Radic Biol Med. 1999 May;26(9-10):1231-7
references.pmid: 10381194
references.citation: Plant Foods Hum Nutr. 2009 Mar;64(1):39-45
references.pmid: 18985454
references.citation: J Food Sci. 2011 May;76(4):C560-7
references.pmid: 22417336
references.citation: Plant Foods Hum Nutr. 2009 Mar;64(1):68-74
references.pmid: 19067170
references.citation: J Agric Food Chem. 2007 Sep 19;55(19):7676-81
references.pmid: 17708649
references.citation: J Agric Food Chem. 2005 Mar 23;53(6):1841-56
references.pmid: 15769103
references.citation: J Agric Food Chem. 2003 May 21;51(11):3273-9
references.pmid: 12744654
references.citation: Plant Foods Hum Nutr. 2001;56(4):359-64
references.pmid: 11678441