title: Identification of three novel avian beta-defensins from goose and their significance in the pathogenesis of Salmonella.
abstract: Here, we report the characterization of three avian β-defensins (AvBDs) from the goose, named anser_AvBD1, AvBD3, and AvBD6, respectively. All of anser_AvBDs exhibited broad antibacterial activity. In addition, the antibacterial activity of all of the AvBDs against Staphylococcus aureus and Proteus mirabilis decreased significantly in the presence of 100mM NaCl (P<0.01). None of the AvBDs showed hemolytic activity. In order to assess the significance of these anser_AvBDs in the infection of Salmonella enteritidis, mRNA expression of Toll-like receptor (TLR) 4 and anser_AvBDs in tissues of both control and infected geese was evaluated. We observed a significant up-regulation of TLR4, anser_AvBD1, 3, and 6 in some immune tissues evaluated, in response to S. enteritidis infection. These data demonstrated that TLR4 may serve a possible role in eliciting host immune responses to pathogens, and β-defensins may play a pivotal role in the host defense mechanisms of the goose.
authors.affiliation: College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, PR China. Electronic address: mdy296@sohu.com.
authors.forename: Deying
authors.initials: D
authors.lastname: Ma
authors.forename: Mingyue
authors.initials: M
authors.lastname: Zhang
authors.forename: Kexin
authors.initials: K
authors.lastname: Zhang
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Liu
authors.forename: Zongxi
authors.initials: Z
authors.lastname: Han
authors.forename: Yuhao
authors.initials: Y
authors.lastname: Shao
authors.forename: Shengwang
authors.initials: S
authors.lastname: Liu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D030161:Avian Proteins / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005777:Geese / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D015870:Gene Expression; D006090:Gram-Negative Bacteria / Q000187:drug effects; D006094:Gram-Positive Bacteria / Q000187:drug effects; D054884:Host-Pathogen Interactions / Q000276:immunology; D050296:Microbial Viability / Q000187:drug effects; D008969:Molecular Sequence Data; D020033:Protein Isoforms / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D011994:Recombinant Proteins / Q000494:pharmacology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012481:Salmonella Infections, Animal / Q000276:immunology* / Q000382:microbiology; D012477:Salmonella enteritidis / Q000276:immunology* / Q000502:physiology; D017386:Sequence Homology, Amino Acid; D051193:Toll-Like Receptors / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D023083:beta-Defensins / Q000235:genetics / Q000276:immunology* / Q000378:metabolism
chemical_list: D030161:Avian Proteins; D020033:Protein Isoforms; D011994:Recombinant Proteins; D051193:Toll-Like Receptors; D023083:beta-Defensins
keywords: Antibacterial activity; Avian β-defensins; Goose; S. enteritidis infection; TLR4