title: Nanoparticles-based multi-adjuvant whole cell tumor vaccine for cancer immunotherapy.
abstract: Whole cell tumor vaccine (WCTV), as a potential treatment modality, elicits limited immune responses because of the poor immunogenicity. To address this issue, researchers have attempted to transduce a cytokine adjuvant into tumor cells, but these single-adjuvant WCTVs curtail the high expectations. In present study, we constructed a multi-adjuvant WCTV based on the nanoparticles modified with cell penetrating peptide, which could facilitate the transportation of granulocyte macrophage colony-stimulating factor (GM-CSF) and interleukin 2 (IL-2) into tumor cells. After inactivation, as-designed multi-adjuvant WCTV exhibited programmed promotions on DC recruitment, antigen presentation, and T-cell activation. In vivo evaluations demonstrated the satisfactory effects on tumor growth suppression, metastasis inhibition, and recurrence prevention. Therefore, the nanoparticles-based multi-adjuvant WCTV may serve as a high-performance treatment for anti-tumor immunotherapy.
authors.affiliation: National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, PR China.
authors.forename: Shi-Ying
authors.initials: SY
authors.lastname: Liu
authors.forename: Wei
authors.initials: W
authors.lastname: Wei
authors.forename: Hua
authors.initials: H
authors.lastname: Yue
authors.forename: De-Zhi
authors.initials: DZ
authors.lastname: Ni
authors.forename: Zhan-Guo
authors.initials: ZG
authors.lastname: Yue
authors.forename: Shuang
authors.initials: S
authors.lastname: Wang
authors.forename: Qiang
authors.initials: Q
authors.lastname: Fu
authors.forename: Yue-Qi
authors.initials: YQ
authors.lastname: Wang
authors.forename: Guang-Hui
authors.initials: GH
authors.lastname: Ma
authors.forename: Zhi-Guo
authors.initials: ZG
authors.lastname: Su
mesh_terms: D000818:Animals; D019496:Cancer Vaccines / Q000627:therapeutic use*; D045744:Cell Line, Tumor; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000008:administration & dosage / Q000627:therapeutic use*; D006801:Humans; D007167:Immunotherapy / Q000379:methods*; D007376:Interleukin-2 / Q000008:administration & dosage / Q000627:therapeutic use*; D051379:Mice; D008810:Mice, Inbred C57BL; D053758:Nanoparticles / Q000008:administration & dosage / Q000737:chemistry*; D011897:Random Allocation
chemical_list: D019496:Cancer Vaccines; D007376:Interleukin-2; D016178:Granulocyte-Macrophage Colony-Stimulating Factor
keywords: Adjuvant; Cytokine; Nanoparticle; Whole cell tumor vaccine