title: Phyto-SERM constitutes from Flemingia macrophylla.
abstract: The methanolic extract of Flemingia macrophylla roots exhibited significant estrogenic activity in the transgenic plant assay system which was comparable to the activity of soybean extract. Utilizing estrogenic activity-guided fractionation, one new compound, fleminigin, together with 23 known compounds were isolated from F. macrophylla roots' methanolic extract. The structure of the new compound was identified based on intensive spectroscopic analysis and the full spectral data for one of the isolated compounds, flemichin E, was introduced for the first time in the current investigation. The estrogenic and anti-estrogenic activities of the isolated compounds were evaluated revealing that the isolated isoflavonoids may act as partial estrogen agonists, as well as antagonists. Additionally, the anti-inflammatory and the cytotoxic activities of the isolated compounds were studied. These results suggested the potential applications of F. macrophylla extract and its isolated compounds as selective estrogen receptor modulators (SERMs).
authors.affiliation: Graduate Institute of Natural Products, College of Pharmacy, Kaohsiung Medical University, Kaohsiung 80708, Taiwan. stellapple7@gmail.com
authors.forename: Wan-Chun
authors.initials: WC
authors.lastname: Lai
authors.forename: Ya-Ting
authors.initials: YT
authors.lastname: Tsui
authors.forename: Abdel Nasser B
authors.initials: AN
authors.lastname: Singab
authors.forename: Mohamed
authors.initials: M
authors.lastname: El-Shazly
authors.forename: Ying-Chi
authors.initials: YC
authors.lastname: Du
authors.forename: Tsong-Long
authors.initials: TL
authors.lastname: Hwang
authors.forename: Chin-Chung
authors.initials: CC
authors.lastname: Wu
authors.forename: Ming-Hong
authors.initials: MH
authors.lastname: Yen
authors.forename: Ching-Kuo
authors.initials: CK
authors.lastname: Lee
authors.forename: Ming-Feng
authors.initials: MF
authors.lastname: Hou
authors.forename: Yang-Chang
authors.initials: YC
authors.lastname: Wu
authors.forename: Fang-Rong
authors.initials: FR
authors.lastname: Chang
mesh_terms: D000893:Anti-Inflammatory Agents / Q000737:chemistry* / Q000302:isolation & purification / Q000633:toxicity; D002470:Cell Survival / Q000187:drug effects; D007887:Fabaceae / Q000737:chemistry*; D006801:Humans; D061986:MCF-7 Cells; D009682:Magnetic Resonance Spectroscopy; D008968:Molecular Conformation; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D018517:Plant Roots / Q000737:chemistry; D011960:Receptors, Estrogen / Q000235:genetics / Q000378:metabolism*; D013481:Superoxides / Q000378:metabolism; D014158:Transcription, Genetic / Q000187:drug effects
chemical_list: D000893:Anti-Inflammatory Agents; D010936:Plant Extracts; D011960:Receptors, Estrogen; D013481:Superoxides
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