title: Aporphine alkaloids from the leaves of Phoebe grandis (Nees) Mer. (Lauraceae) and their cytotoxic and antibacterial activities.
abstract: The oxoaporphine alkaloid lysicamine (1), and three proaporphine alkaloids, litsericinone (2), 8,9,11,12-tetrahydromecambrine (3) and hexahydromecambrine A (4) were isolated from the leaves of Phoebe grandis (Nees) Merr. (Lauraceae). Compounds 2 and 3 were first time isolated as new naturally occurring compounds from plants. The NMR data for the compounds 2-4 have never been reported so far. Compounds 1 and 2 showed significant cytotoxic activity against a MCF7 (human estrogen receptor (ER+) positive breast cancer) cell line with IC₅₀ values of 26 and 60 µg/mL, respectively. Furthermore, in vitro cytotoxic activity against HepG2 (human liver cancer) cell line was evaluated for compounds 1-4 with IC₅₀ values of 27, 14, 81 and 20 µg/mL, respectively. Lysicamine (1) displayed strong antibacterial activity against Bacillus subtilis (B145), Staphylococcus aureus (S1434) and Staphylococus epidermidis (a clinically isolated strain) with inhibition zones of 15.50 ± 0.57, 13.33 ± 0.57 and 12.00 ± 0.00 mm, respectively. However, none of the tested pathogenic bacteria were susceptible towards compounds 2 and 3.
authors.affiliation: Chemistry Department, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia. hanita74@um.edu.my
authors.forename: Hanita
authors.initials: H
authors.lastname: Omar
authors.forename: Najihah Mohd
authors.initials: NM
authors.lastname: Hashim
authors.forename: Asdren
authors.initials: A
authors.lastname: Zajmi
authors.forename: Noraziah
authors.initials: N
authors.lastname: Nordin
authors.forename: Siddiq Ibrahim
authors.initials: SI
authors.lastname: Abdelwahab
authors.forename: Ainnul Hamidah Syahadah
authors.initials: AH
authors.lastname: Azizan
authors.forename: A Hamid A
authors.initials: AH
authors.lastname: Hadi
authors.forename: Hapipah Mohd
authors.initials: HM
authors.lastname: Ali
mesh_terms: D000470:Alkaloids / Q000008:administration & dosage* / Q000737:chemistry / Q000302:isolation & purification; D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000737:chemistry; D001060:Aporphines / Q000008:administration & dosage* / Q000737:chemistry / Q000302:isolation & purification; D001419:Bacteria / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D003603:Cytotoxins / Q000008:administration & dosage / Q000737:chemistry; D056945:Hep G2 Cells / Q000187:drug effects; D006801:Humans; D061986:MCF-7 Cells / Q000187:drug effects; D010936:Plant Extracts / Q000008:administration & dosage / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry; D018539:Saturn
chemical_list: D000470:Alkaloids; D000900:Anti-Bacterial Agents; D001060:Aporphines; D003603:Cytotoxins; D010936:Plant Extracts
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