title: The selectable antibiotic marker, tetA(C), increases Pseudomonas aeruginosa susceptibility to the herbicide/superoxide generator, paraquat.
abstract: The presence of the widely used selectable antibiotic marker, tetA(C), unexpectedly increased the sensitivity of Pseudomonas aeruginosa PAO1 to the superoxide-generating herbicide, paraquat. A DNA fragment spanning the first 99 amino acids of TetA(C) was sufficient to confer paraquat sensitivity. The TetA(C)-induced paraquat sensitive phenotype was observed in other Gram-negative bacteria such as Agrobacterium tumefaciens, Salmonella enterica ser. Typhimurium and Xanthomonas campestris suggesting that this is a general property of tetA(C). This finding serves as a cautionary note for those using tetA(C) as a selectable marker for genetic manipulations in studies using paraquat either as a superoxide stress generator or a redox cycling drug.
authors.affiliation: Molecular Medicine Graduate Program, Faculty of Science, Mahidol University, Bangkok, Thailand.
authors.forename: Panithi
authors.initials: P
authors.lastname: Leesukon
authors.forename: Wilaiwan
authors.initials: W
authors.lastname: Wirathorn
authors.forename: Tatsanee
authors.initials: T
authors.lastname: Chuchue
authors.forename: Nisanart
authors.initials: N
authors.lastname: Charoenlap
authors.forename: Skorn
authors.initials: S
authors.lastname: Mongkolsuk
mesh_terms: D017920:Antiporters / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D006540:Herbicides / Q000494:pharmacology*; D010084:Oxidation-Reduction; D010269:Paraquat / Q000378:metabolism* / Q000494:pharmacology; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000378:metabolism*; D013481:Superoxides / Q000378:metabolism
chemical_list: D017920:Antiporters; D001426:Bacterial Proteins; D006540:Herbicides; C079051:tetA protein, Bacteria; D013481:Superoxides; D010269:Paraquat