title: Role of a GntR-family response regulator LbrA in Listeria monocytogenes biofilm formation.
abstract: The formation of Listeria monocytogenes biofilms contributes to persistent contamination in food processing facilities. A microarray comparison of L. monocytogenes between the transcriptome of the strong biofilm forming strain (Bfm(s)) Scott A and the weak biofilm forming (Bfm(w)) strain F2365 was conducted to identify genes potentially involved in biofilm formation. Among 951 genes with significant difference in expression between the two strains, a GntR-family response regulator encoding gene (LMOf2365_0414), designated lbrA, was found to be highly expressed in Scott A relative to F2365. A Scott A lbrA-deletion mutant, designated AW3, formed biofilm to a much lesser extent as compared to the parent strain by a rapid attachment assay and scanning electron microscopy. Complementation with lbrA from Scott A restored the Bfm(s) phenotype in the AW3 derivative. A second microarray assessment using the lbrA deletion mutant AW3 and the wild type Scott A revealed a total of 304 genes with expression significantly different between the two strains, indicating the potential regulatory role of LbrA in L. monocytogenes. A cloned copy of Scott A lbrA was unable to confer enhanced biofilm forming potential in F2365, suggesting that additional factors contributed to weak biofilm formation by F2365.
authors.affiliation: Department of Food Science and Technology, The Ohio State University, Columbus, Ohio, USA.
authors.forename: Andrew
authors.initials: A
authors.lastname: Wassinger
authors.forename: Lu
authors.initials: L
authors.lastname: Zhang
authors.forename: Erin
authors.initials: E
authors.lastname: Tracy
authors.forename: Robert S
authors.initials: RS
authors.lastname: Munson
authors.forename: Sophia
authors.initials: S
authors.lastname: Kathariou
authors.forename: Hua H
authors.initials: HH
authors.lastname: Wang
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism*; D018441:Biofilms; D005516:Food Microbiology; D015964:Gene Expression Regulation, Bacterial; D005816:Genetic Complementation Test; D008089:Listeria monocytogenes / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D017384:Sequence Deletion / Q000235:genetics; D059467:Transcriptome
chemical_list: D001426:Bacterial Proteins
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