title: Study on the in vitro effects of the mixtures of polycyclic aromatic hydrocarbons (PAHs) and heavy metals on ethoxyresorufin-O-deethylase (EROD) activity in Mossambica tilapia liver.
abstract: This paper reports in vitro effects of individual heavy metals (Cd(2+), Cu(2+) and Hg(2+)), and PAHs, including benzo[a]pyrene(BaP), indeno[1,2,3-cd]pyrene (IP) and fluoranthene (FL), and their mixtures on ethoxyresorufin-O-deethylase (EROD) activities using a plate-reader method. The results showed that all three metals inhibited EROD activity, while BaP/IP significantly induced the enzyme. However, FL alone decreased EROD activity. Moreover, co-treatment with BaP/IP and heavy metals inhibited PAH-induced EROD activities, while combined exposure to FL and heavy metals induced FL-inhibited EROD activities.
authors.affiliation: School of Pharmaceutical Sciences, Xiamen University, Xiamen, 361005, China.
authors.forename: Da Xiong
authors.initials: da X
authors.lastname: Han
authors.forename: Hai Yan
authors.initials: HY
authors.lastname: Wang
authors.forename: Hong Wei
authors.initials: HW
authors.lastname: Yue
authors.forename: Su Min
authors.initials: SM
authors.lastname: Wang
mesh_terms: D000818:Animals; D019363:Cytochrome P-450 CYP1A1 / Q000378:metabolism*; D008099:Liver / Q000187:drug effects / Q000201:enzymology*; D019216:Metals, Heavy / Q000633:toxicity*; D011084:Polycyclic Aromatic Hydrocarbons / Q000633:toxicity*; D017210:Tilapia / Q000502:physiology; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D019216:Metals, Heavy; D011084:Polycyclic Aromatic Hydrocarbons; D014874:Water Pollutants, Chemical; D019363:Cytochrome P-450 CYP1A1