title: Evaluation of immunohistochemical expression of ghrelin-producing rectal cells in Wistar rats receiving the cafeteria diet.
abstract: OBJECTIVE
  To investigate the impact of cafeteria diet on ghrelin expression in rectal tissue and identify the morphologic cell type.
  
  
  METHODS
  Twenty-four male Wistar rats were divided into four subgroups of six animals each: RC1 (rat chow 1) and CAF1 (cafeteria diet 1) for a period of 30 days; RC2 (rat chow 2) and CAF2 (cafeteria diet 2) for a period of 60 days. The animal and rectal weight, the number and the type of immunoreactive ghrelin cells were recorded and compared between the subgroups. The statistical study was established by ANOVA and Student's t test.
  
  
  RESULTS
  There was no difference in the total of immunoreactive cells (p=0.685) between the subgroups nor between weight and presence or absence of ghrelin expression (p=0.993). All the immunoreactive cells identified were closed-type.
  
  
  CONCLUSIONS
  The cafeteria diet did not have influence on the amount of immunoreactive rectal cells of ghrelin and only one type (closed-type) of immunoreactive cells was expressed in the rectum.
authors.affiliation: Postgraduate Program in Medical Sciences, Department of Surgery, University Hospital, Federal University of Santa Catarina, Florianopolis, SC, Brazil. humbertolyrajr@gmail.com
authors.forename: Humberto Fenner
authors.initials: HF
authors.lastname: Lyra
authors.forename: Leonardo de Lucca
authors.initials: Lde L
authors.lastname: Schiavon
authors.forename: Daniella Serafin Couto
authors.initials: DS
authors.lastname: Vieira
authors.forename: André Muller
authors.initials: AM
authors.lastname: Teive
authors.forename: Aline
authors.initials: A
authors.lastname: Costa
authors.forename: Tiago Rafael
authors.initials: TR
authors.lastname: Onzi
authors.forename: Angélica Luciana
authors.initials: AL
authors.lastname: Nau
authors.forename: Armando José
authors.initials: AJ
authors.lastname: d'Acampora
mesh_terms: D000818:Animals; D002452:Cell Count; D004032:Diet; D004040:Dietary Carbohydrates / Q000378:metabolism*; D054439:Ghrelin / Q000096:biosynthesis*; D007150:Immunohistochemistry; D008297:Male; D009929:Organ Size; D051381:Rats; D017208:Rats, Wistar; D012007:Rectum / Q000166:cytology*; D012016:Reference Values; D013997:Time Factors
chemical_list: D004040:Dietary Carbohydrates; D054439:Ghrelin