title: (1→3)-β-D-Glucan reduces the damages caused by reactive oxygen species induced in human platelets by lipopolysaccharides.
abstract: LPS (lipopolysaccharide) induces platelet activation and is a well-known fundamental agent of septic shock and disseminated intravascular coagulation (DIC). Biological activity of (1→3)-β-D-glucan is related due to its anti-inflammatory, antioxidant, and antitumor properties. We focus our attention on the (1→3)-β-D-glucan (antiplatelet) properties. The main purpose of our study was to evaluate the influence of (1→3)-β-D-glucan from Saccharomyces cerevisiae on destructive activity of LPS (from Escherichia coli and Pseudomonas aeruginosa) on human blood platelets. We assess biochemically in vitro if (1→3)-β-D-glucan might combat the oxidative stress caused by LPS stroke associated with nitrative and oxidative damages of human platelet biomolecules. We also make an attempt by in silico molecular docking to determine the interactions between the molecules of (1→3)-β-D-glucan and LPS. Our conclusion is that protective mechanism of (1→3)-β-D-glucan against LPS action on blood platelets is due to as well: its antioxidant properties, as to its interaction with LPS-binding region of TLR4-MD-2 complex.
authors.affiliation: Department of General Biochemistry, Faculty of Biology and Environmental Protection, University of Lodz, Pomorska 141/143, 90-236 Lodz, Poland. juszczak@biol.uni.lodz.pl
authors.forename: Joanna
authors.initials: J
authors.lastname: Saluk
authors.forename: Michał
authors.initials: M
authors.lastname: Bijak
authors.forename: Michał Błażej
authors.initials: MB
authors.lastname: Ponczek
authors.forename: Paweł
authors.initials: P
authors.lastname: Nowak
authors.forename: Barbara
authors.initials: B
authors.lastname: Wachowicz
mesh_terms: D000328:Adult; D001792:Blood Platelets / Q000187:drug effects / Q000378:metabolism*; D001798:Blood Proteins / Q000378:metabolism; D019295:Computational Biology; D006801:Humans; D008070:Lipopolysaccharides / Q000737:chemistry / Q000633:toxicity*; D008297:Male; D008958:Models, Molecular; D015538:Nitrosation / Q000187:drug effects; D011509:Proteoglycans; D017382:Reactive Oxygen Species / Q000633:toxicity*; D013481:Superoxides / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D051197:Toll-Like Receptor 4 / Q000737:chemistry / Q000378:metabolism; D014443:Tyrosine / Q000378:metabolism; D055815:Young Adult; D047071:beta-Glucans / Q000494:pharmacology*
chemical_list: D001798:Blood Proteins; D008070:Lipopolysaccharides; D011509:Proteoglycans; D017382:Reactive Oxygen Species; C495345:TLR4 protein, human; D017392:Thiobarbituric Acid Reactive Substances; D051197:Toll-Like Receptor 4; D047071:beta-Glucans; D013481:Superoxides; C010770:polysaccharide-K; D014443:Tyrosine
keywords: (1→3)-β-d-Glucan; Blood platelets; Endotoxin; Lipopolysaccharide; TLR-4