title: Assessment of an elastin binding molecule for PET imaging of atherosclerotic plaques.
abstract: Elastin is considered as a key player in human vascular diseases and it might contribute to the development of atherosclerosis. The elastin binding radiotracer, [(18)F]AlF-NOTA-EBM ([(18)F]2), was evaluated in a wild type mouse to determine its in vivo distribution and on human carotid atherosclerotic plaque tissues to assess its utility as a PET imaging agent for visualizing human atherosclerotic plaque lesions. The free ligand NOTA-EBM, which served as the precursor, was obtained in 25% chemical yield. The radiosynthesis of [(18)F]2 was accomplished by coordination of Al(18)F to NOTA-EBM in 8-13% decay corrected radiochemical yield (n = 7) and specific radioactivity of 59 ± 12 GBq/μmol. A dynamic in vivo PET scan in a healthy wild type mouse (C57BL/6) showed high accumulation of radioactivity in heart and lungs, organs reported to have high elastin content. Excretion of [(18)F]2 proceeded via the renal pathway and through the hepatobiliary system as indicated by a high uptake of radioactivity in the liver, intestines and gall bladder. In vitro autoradiography on human atherosclerotic plaque sections showed a heterogeneous distribution of [(18)F]2 with an elevated accumulation in stable and vulnerable atherosclerotic plaques compared to control samples of normal arteries. However, there was no statistical significance between the different plaque phenotypes and control samples. Competition experiments with 10.000-fold excess of free ligand NOTA-EBM resulted in a marked decrease of radioactivity accumulation, consistent with a target-specific ligand.
authors.affiliation: Center for Radiopharmaceutical Sciences of ETH, PSI and USZ, Department of Chemistry and Applied Biosciences of ETH Zurich Wolfgang-Pauli Strasse 10, CH-8093 Zurich, Switzerland.
authors.forename: Cindy R
authors.initials: CR
authors.lastname: Fischer
authors.forename: Adrienne
authors.initials: A
authors.lastname: Müller
authors.forename: Bianca
authors.initials: B
authors.lastname: Bochsler
authors.forename: Zoran
authors.initials: Z
authors.lastname: Rancic
authors.forename: Philipp
authors.initials: P
authors.lastname: Kaufmann
authors.forename: Roger
authors.initials: R
authors.lastname: Schibli
authors.forename: Simon M
authors.initials: SM
authors.lastname: Ametamey
keywords: Al18F; Elastin; PET imaging; atherosclerotic plaques; autoradiography
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