title: [Activities of treg cells stimulated by soluble adult worm antigen and egg antigen of Schistosoma japonicum].
abstract: OBJECTIVE
  To observe and compare the effects of soluble adult worm antigen (SWA) and soluble egg antigen (SEA) of Schistosoma japonicum on the induction of Treg cells and the suppressive activity of Treg cells.
  
  
  METHODS
  Splenocytes were prepared from mice treated with PBS, SWA, and SEA, respectively, and then the proportions of Treg cells and the levels of IL-10 and TGF-beta in Treg cells were determined by FACS. The purified Treg cells from the mice treated as above-mentioned were detected for their immunosuppressive activities by incorporation of [3H] thymidine for the final 16 h of culture.
  
  
  RESULTS
  Compared to SWA, SEA induced the higher proportion of Treg cells with a stronger suppressive activity, which produced the higher levels of IL-10 and TGF-beta (P < 0.05).
  
  
  CONCLUSIONS
  SEA significantly induces Treg cells and enhances their immunosuppressive activity.
authors.affiliation: Department of Pathogen Biology, Nanjing Medical University, Nanjing 210029, China.
authors.forename: Xiao-Xiao
authors.initials: XX
authors.lastname: Dong
authors.forename: Cui
authors.initials: C
authors.lastname: Zhang
authors.forename: Xiao-Wei
authors.initials: XW
authors.lastname: Yang
authors.forename: Yong
authors.initials: Y
authors.lastname: Li
authors.forename: Xiao-Jun
authors.initials: XJ
authors.lastname: Chen
authors.forename: Xue
authors.initials: X
authors.lastname: Xue
authors.forename: Wei-Wei
authors.initials: WW
authors.lastname: Zhang
authors.forename: Zhi-Peng
authors.initials: ZP
authors.lastname: Xu
authors.forename: Wen-Jun
authors.initials: WJ
authors.lastname: Kong
authors.forename: Ji-Feng
authors.initials: JF
authors.lastname: Zhu
authors.forename: Sha
authors.initials: S
authors.lastname: Zhou
authors.forename: Feng
authors.initials: F
authors.lastname: Liu
authors.forename: Chuan
authors.initials: C
authors.lastname: Su
mesh_terms: D000818:Animals; D000947:Antigens, Helminth / Q000276:immunology*; D005260:Female; D016753:Interleukin-10 / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D010063:Ovum / Q000276:immunology*; D012549:Schistosoma japonicum / Q000276:immunology*; D012554:Schistosomiasis japonica / Q000276:immunology* / Q000469:parasitology; D013154:Spleen / Q000276:immunology / Q000469:parasitology; D050378:T-Lymphocytes, Regulatory / Q000276:immunology* / Q000469:parasitology; D016212:Transforming Growth Factor beta / Q000276:immunology
chemical_list: D000947:Antigens, Helminth; D016212:Transforming Growth Factor beta; D016753:Interleukin-10