title: The role of 3D structure and protein conformation on the innate and adaptive immune responses to silk-based biomaterials.
abstract: We have investigated monocyte and T cell responsiveness to silk based biomaterials of different physico-chemical characteristics. Here we report that untransformed CD14+ human monocytes respond to overnight exposure to silk fibroin-based biomaterials in tridimensional form by IL-1β and IL-6, but not IL-10 gene expression and protein production. In contrast, fibroin based materials in bidimensional form are unable to stimulate monocyte responsiveness. The elicitation of these effects critically requires contact between biomaterials and responding cells, is not sustained and becomes undetectable in longer term cultures. We also observed that NF-κβ and p38 MAP kinase play key roles in monocyte activation by silk-based biomaterials. On the other hand, fibroin based materials, irrespective of their physico-chemical characteristics appeared to be unable to induce the activation of peripheral blood T cells from healthy donors, as evaluated by the expression of activation markers and IFN-γ gene.
authors.affiliation: Department of Textile Technology, Indian Institute of Technology, New Delhi 110016, India.
authors.forename: Maumita
authors.initials: M
authors.lastname: Bhattacharjee
authors.forename: Elke
authors.initials: E
authors.lastname: Schultz-Thater
authors.forename: Emanuele
authors.initials: E
authors.lastname: Trella
authors.forename: Sylvie
authors.initials: S
authors.lastname: Miot
authors.forename: Sanskrita
authors.initials: S
authors.lastname: Das
authors.forename: Marko
authors.initials: M
authors.lastname: Loparic
authors.forename: Alok R
authors.initials: AR
authors.lastname: Ray
authors.forename: Ivan
authors.initials: I
authors.lastname: Martin
authors.forename: Giulio C
authors.initials: GC
authors.lastname: Spagnoli
authors.forename: Sourabh
authors.initials: S
authors.lastname: Ghosh
mesh_terms: D056704:Adaptive Immunity / Q000187:drug effects*; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry* / Q000494:pharmacology*; D002478:Cells, Cultured; D016207:Cytokines / Q000378:metabolism; D006801:Humans; D007113:Immunity, Innate / Q000187:drug effects*; D016753:Interleukin-10 / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D018625:Microscopy, Atomic Force; D009000:Monocytes / Q000187:drug effects / Q000378:metabolism; D016328:NF-kappa B / Q000378:metabolism; D047011:Silk / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D001672:Biocompatible Materials; D016207:Cytokines; D015850:Interleukin-6; D016328:NF-kappa B; D047011:Silk; D016753:Interleukin-10
keywords: Biocompatibility; Cytokines; Monocyte activation; Silk-based biomaterials; Structural architectures