title: A method for the ultrastructural preservation of tiny percutaneous needle biopsy material from skeletal muscle.
abstract: Skeletal muscle biopsies require transecting the muscle fibers resulting, in structural damage near the cut ends. Classically, the optimal ultrastructural preservation has been obtained by the use of relatively large biopsies in which the tissue fibers are restrained by ligating to a suitable retaining support prior to excision, and by examining regions at some distance from the cut ends. However, these methods require invasive surgical procedures. In the present study, we present and substantiate an alternative approach that allows for the excellent ultrastructural preservation of needle biopsy samples, even the very small samples obtained through tiny percutaneous needle biopsy (TPNB). TPNB represents an advantage, relative to standard muscle biopsy techniques and to other needle biopsies currently in use, as in addition to not requiring a skin incision, it leaves no scars in the muscle and requires an extremely brief recovery period. It is most appropriate for obtaining repeated samples in horizontal studies, e.g., in order to follow changes with athletic training and/or aging in a single individual and for studies of sarcopenic muscles in elderly patients. Due to the small size of the sample, TPNB may present limited usefulness for classical pathology diagnostics. However, it offers the major advantage of allowing multiple samples within a single session and this may be useful under specific circumstances.
authors.affiliation: Department of Neuroscience and Imaging, Section of Physiology and Physiopathology, G. d'Annunzio University of Chieti-Pescara and Clinical Research Center of G. d'Annunzio Foundation, Chieti I-66100, Italy.
authors.forename: Tiziana
authors.initials: T
authors.lastname: Pietrangelo
authors.forename: Stefano
authors.initials: S
authors.lastname: Perni
authors.forename: Guglielmo
authors.initials: G
authors.lastname: Di Tano
authors.forename: Giorgio
authors.initials: G
authors.lastname: Fanò-Illic
authors.forename: Clara
authors.initials: C
authors.lastname: Franzini-Armstrong
mesh_terms: D000328:Adult; D000368:Aged; D001707:Biopsy, Needle / Q000379:methods*; D006801:Humans; D008297:Male; D008846:Micromanipulation / Q000379:methods*; D008854:Microscopy, Electron; D008875:Middle Aged; D018482:Muscle, Skeletal / Q000473:pathology* / Q000648:ultrastructure
references.citation: J Physiol. 1959 Oct;148:127-60
references.pmid: 14402240
references.citation: Int J Mol Med. 2009 Oct;24(4):503-12
references.pmid: 19724891
references.citation: J Biol Regul Homeost Agents. 2011 Oct-Dec;25(4):635-45
references.pmid: 22217995
references.citation: Scand J Clin Lab Invest. 1975 Nov;35(7):609-16
references.pmid: 1108172
references.citation: Mol Biol Cell. 2009 Feb;20(3):1058-67
references.pmid: 19037102
references.citation: Science. 1969 Dec 5;166(3910):1297-8
references.pmid: 5350329
references.citation: Aging Cell. 2010 Dec;9(6):958-70
references.pmid: 20961389
references.citation: Exp Gerontol. 2004 Jan;39(1):17-24
references.pmid: 14724060
references.citation: Int J Immunopathol Pharmacol. 2009 Jul-Sep;22(3):795-807
references.pmid: 19822097
references.citation: Int J Mol Med. 2011 Mar;27(3):361-7
references.pmid: 21165550