title: Ciceribacter lividus gen. nov., sp. nov., isolated from rhizosphere soil of chick pea (Cicer arietinum L.).
abstract: The taxonomic position of strain MSSRFBL1(T), isolated from chickpea rhizosphere soil from Kannivadi, India, was determined. Strain MSSRFBL1(T) formed bluish black colonies, stained Gram-negative and was motile, aerobic, capable of fixing dinitrogen, oxidase-negative and catalase-positive. Q-10 was the major respiratory quinone. Major fatty acids of strain MSSRFBL1(T) were C18 : 1ω7c and C19 : 0cycloω8c. Minor amounts of C18 : 0, C12 : 0, C14 : 0 3-OH, C18 : 0 3-OH, C16 : 0, C16 : 1ω6c/C16 : 1ω7c, C17 : 0 3-OH and C20 : 1ω7c were also present. Polar lipids included diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylmethylethanolamine, phosphatidylcholine and two unidentified glycolipids. Bacteriohopane derivatives (BHD1 and 2), diplopterol, diploptene, bishomohopanediol, adenosylhopane and 2β-methyl bacteriohopanetetrol were the major hopanoids of strain MSSRFBL1(T). The genomic DNA G+C content was 71 mol%. EzTaxon-e-based blast analysis of the 16S rRNA gene indicated the highest similarity of strain MSSRFBL1(T) to Ensifer adhaerens LMG 20216(T) (97.3 %) and other members of the genus Ensifer (<96.9 %) in the family Rhizobiaceae of the class Alphaproteobacteria. However, phylogenetic analysis based on 16S rRNA, recA, thrC and dnaK gene sequences showed distinct out-grouping from the recognized genera of the family Rhizobiaceae. Based on phenotypic, genotypic and chemotaxonomic characters, strain MSSRFBL1(T) represents a novel species in a new genus in the family Rhizobiaceae for which the name Ciceribacter lividus gen. nov., sp. nov. is proposed. The type strain of Ciceribacter lividus is MSSRFBL1(T) ( = DSM 25528(T) = KCTC 32403(T)).
authors.affiliation: Microbiology Department, M. S. Swaminathan Research Foundation, 3rd Cross street, Taramani institutional area, Chennai 600113, India.
authors.forename: R
authors.initials: R
authors.lastname: Kathiravan
authors.affiliation: Microbiology Department, M. S. Swaminathan Research Foundation, 3rd Cross street, Taramani institutional area, Chennai 600113, India.
authors.forename: S
authors.initials: S
authors.lastname: Jegan
authors.affiliation: Microbiology Department, M. S. Swaminathan Research Foundation, 3rd Cross street, Taramani institutional area, Chennai 600113, India.
authors.forename: V
authors.initials: V
authors.lastname: Ganga
authors.affiliation: Microbiology Department, M. S. Swaminathan Research Foundation, 3rd Cross street, Taramani institutional area, Chennai 600113, India.
authors.forename: V R
authors.initials: VR
authors.lastname: Prabavathy
authors.affiliation: Department of Plant Sciences, School of Life Sciences, University of Hyderabad, PO Central University, Gachibowli, Hyderabad 500046, India.
authors.forename: L
authors.initials: L
authors.lastname: Tushar
authors.affiliation: Bacterial Discovery laboratory, Centre for Environment, Institute of Science and Technology, JNT University Hyderabad, Kukatpally, Hyderabad 500085, India.
authors.forename: Ch
authors.initials: C
authors.lastname: Sasikala
authors.affiliation: Department of Plant Sciences, School of Life Sciences, University of Hyderabad, PO Central University, Gachibowli, Hyderabad 500046, India.
authors.forename: Ch V
authors.initials: CV
authors.lastname: Ramana
mesh_terms: D020561:Alphaproteobacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D001482:Base Composition; D029863:Cicer / Q000382:microbiology*; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D005798:Genes, Bacterial; D006017:Glycolipids / Q000737:chemistry; D007194:India; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D058441:Rhizosphere; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D014315:Triterpenes / Q000737:chemistry; D014451:Ubiquinone / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D006017:Glycolipids; D012336:RNA, Ribosomal, 16S; D014315:Triterpenes; D014451:Ubiquinone; C025204:Ubiquinone Q2