title: Effect of diclofenac and antidepressants on the inflammatory response in astrocyte cell culture.
abstract: Central nervous system (CNS) has a completely separate immune system that communicates with the neurons by small molecules called cytokines. Cytokines are involved in many crucial processes in neuron including cell metabolism and neurotransmitter synthesis. It has been reported that cytokine imbalance is involved in the progression of many CNS diseases such as neuropsychiatric disorders (depression, schizophrenia, autism, and bipolar disorder) and neurodegenerative disorders (Parkinson's and Alzheimer's disease). Here, the effects of diclofenac, different antidepressants (sertraline, venlafaxine, and fluvoxamine), and vitamin B₆ (pyridoxine) on IL-10 and tumor necrosis factor-α (TNF-α) change with and without immune challenges with lipopolysaccharide (LPS) were investigated in in vitro culture of astrocytes from 2-day-old Swiss-Albino mice. Diclofenac and Sertraline significantly (p < 0.05) improves anti-inflammatory cytokine (IL-10) while suppress (p < 0.05) LPS-induced elevated level of pro-inflammatory mediators (TNF-α) in astrocyte culture. Pyridoxine was not able to reduce (p > 0.05) TNF-α in the astrocyte culture. Antidepressant (sertraline) showed positive effects (increased IL-10 and reduced TNF-α level) possibly through the suppression of Th1 lymphocytes and monocytes and stimulation of Th2 lymphocytes and monocytes/macrophages. NSAID (diclofenac) showed positive immune regulation effect possibly through the inhibition of cyclo-oxygenase enzyme. Based on these findings, it may conclude that, diclofenac and antidepressants (sertraline) may positively contribute in the cytokine production in astrocyte cell culture.
authors.affiliation: Department of Pharmacy, North South University, Plot-15, Block-B, Bashundhara, Dhaka, 1229, Bangladesh, alamin@northsouth.edu.
authors.forename: Md Mamun
authors.initials: MM
authors.lastname: Al-Amin
authors.forename: Mir Muhammad Nasir
authors.initials: MM
authors.lastname: Uddin
authors.forename: Md Mahbubur
authors.initials: MM
authors.lastname: Rahman
authors.forename: Hasan Mahmud
authors.initials: HM
authors.lastname: Reza
authors.forename: Md Sohel
authors.initials: MS
authors.lastname: Rana
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D000928:Antidepressive Agents / Q000494:pharmacology*; D001253:Astrocytes / Q000166:cytology / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D002478:Cells, Cultured; D003511:Cyclohexanols / Q000494:pharmacology; D004008:Diclofenac / Q000494:pharmacology*; D004797:Enzyme-Linked Immunosorbent Assay; D016666:Fluvoxamine / Q000494:pharmacology; D016753:Interleukin-10 / Q000819:agonists / Q000037:antagonists & inhibitors / Q000378:metabolism; D008070:Lipopolysaccharides / Q000037:antagonists & inhibitors / Q000633:toxicity; D051379:Mice; D011736:Pyridoxine / Q000378:metabolism; D017367:Selective Serotonin Reuptake Inhibitors / Q000494:pharmacology; D020280:Sertraline / Q000494:pharmacology*; D014409:Tumor Necrosis Factor-alpha / Q000819:agonists / Q000037:antagonists & inhibitors / Q000378:metabolism; D000069470:Venlafaxine Hydrochloride
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D000928:Antidepressive Agents; D003511:Cyclohexanols; D004008:Diclofenac; D016666:Fluvoxamine; D016753:Interleukin-10; D008070:Lipopolysaccharides; D011736:Pyridoxine; D017367:Selective Serotonin Reuptake Inhibitors; D020280:Sertraline; D014409:Tumor Necrosis Factor-alpha; D000069470:Venlafaxine Hydrochloride; C556062:IL10 protein, mouse
references.citation: Neurosci Lett. 2008 Aug 15;441(1):29-34
references.pmid: 18584961
references.citation: Glia. 2012 Nov;60(11):1773-84
references.pmid: 22847996
references.citation: Methods Mol Biol. 2012;814:235-49
references.pmid: 22144311
references.citation: Depress Anxiety. 2009;26(7):607-11
references.pmid: 19496103
references.citation: J Immunother. 2009 Jan;32(1):22-8
references.pmid: 19307990
references.citation: Arthritis Res Ther. 2005;7(6):R1404-11
references.pmid: 16277693
references.citation: J Neuroinflammation. 2013 Apr 01;10:43
references.pmid: 23547920
references.citation: Int J Neuropsychopharmacol. 2002 Dec;5(4):375-88
references.pmid: 12466036
references.citation: Immunopharmacology. 1996 Aug;34(1):27-37
references.pmid: 8880223
references.citation: Neuropsychopharmacology. 1999 Apr;20(4):370-9
references.pmid: 10088138
references.citation: Clin Dev Immunol. 2007;2007:76396
references.pmid: 18317531
references.citation: J Neurochem. 1999 Apr;72(4):1466-71
references.pmid: 10098850
references.citation: Infect Immun. 2011 Aug;79(8):3338-47
references.pmid: 21576329
references.citation: J Psychiatr Res. 2008 Dec;43(2):89-97
references.pmid: 18394646
references.citation: Eur J Pharmacol. 2009 Jun 10;612(1-3):54-60
references.pmid: 19356723