title: [11R-VIVIT inhibits the expression of urokinase-type plasminogen activator receptor in podocytes].
abstract: OBJECTIVE
  To observe the effect of 11R-VIVIT on lipopolysaccharide (LPS)-induced expression of urokinase-type plasminogen activator receptor (uPAR) in podocytes.
  
  
  METHODS
  A LPS-induced proteinuria mouse model and in vitro cultured podocytes treated with LPS were both divided into control group, LPS group and LPS+11 R-VIVIT group. The mRNA and protein expressions of uPAR in mouse kidney tissues and the podocytes were measured by real-time qPCR, laser scanning confocal microscopy and Western blotting.
  
  
  RESULTS
  Compared with LPS group, LPS+11 R-VIVIT group showed a significantly lowered urine albumin/creatinine ratio (P<0.001) and markedly reduced mRNA and protein expressions of uPAR (PuPAR mRNA<0.001; PuPAR=0.001).
  
  
  CONCLUSIONS
  11R-VIVIT can ameliorate proteinuria probably by decreasing the expression of uPAR in podocytes.
authors.affiliation: Department of Nephrology, Guangdong Academy of Medical Sciences, Guangzhou, China. 15920309398@139.com
authors.forename: Ruizhao
authors.initials: R
authors.lastname: Li
authors.forename: Wei
authors.initials: W
authors.lastname: Shi
authors.forename: Juan
authors.initials: J
authors.lastname: Ma
authors.forename: Bin
authors.initials: B
authors.lastname: Zhang
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.forename: Xinling
authors.initials: X
authors.lastname: Liang
authors.forename: Yuanhan
authors.initials: Y
authors.lastname: Chen
authors.forename: Shuangxin
authors.initials: S
authors.lastname: Liu
authors.forename: Wenjian
authors.initials: W
authors.lastname: Wang
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D008070:Lipopolysaccharides / Q000009:adverse effects; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009842:Oligopeptides / Q000494:pharmacology*; D050199:Podocytes / Q000187:drug effects* / Q000378:metabolism; D011507:Proteinuria / Q000188:drug therapy* / Q000378:metabolism; D055293:Receptors, Urokinase Plasminogen Activator / Q000378:metabolism*
chemical_list: D008070:Lipopolysaccharides; D009842:Oligopeptides; D055293:Receptors, Urokinase Plasminogen Activator; C121611:VIVIT peptide