title: A role for SIRT2-dependent histone H3K18 deacetylation in bacterial infection.
abstract: Pathogens dramatically affect host cell transcription programs for their own profit during infection, but in most cases, the underlying mechanisms remain elusive. We found that during infection with the bacterium Listeria monocytogenes, the host deacetylase sirtuin 2 (SIRT2) translocates to the nucleus, in a manner dependent on the bacterial factor InlB. SIRT2 associates with the transcription start site of a subset of genes repressed during infection and deacetylates histone H3 on lysine 18 (H3K18). Infecting cells in which SIRT2 activity was blocked or using SIRT2(-/-) mice resulted in a significant impairment of bacterial infection. Thus, SIRT2-mediated H3K18 deacetylation plays a critical role during infection, which reveals an epigenetic mechanism imposed by a pathogenic bacterium to reprogram its host.
authors.affiliation: Unité des Interactions Bactéries-Cellules, Institut Pasteur, Paris, France.
authors.forename: Haig A
authors.initials: HA
authors.lastname: Eskandarian
authors.forename: Francis
authors.initials: F
authors.lastname: Impens
authors.forename: Marie-Anne
authors.initials: MA
authors.lastname: Nahori
authors.forename: Guillaume
authors.initials: G
authors.lastname: Soubigou
authors.forename: Jean-Yves
authors.initials: JY
authors.lastname: Coppée
authors.forename: Pascale
authors.initials: P
authors.lastname: Cossart
authors.forename: Mélanie A
authors.initials: MA
authors.lastname: Hamon
mesh_terms: D000107:Acetylation; D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics; D002467:Cell Nucleus / Q000378:metabolism; D002843:Chromatin / Q000378:metabolism; D003600:Cytosol / Q000378:metabolism; D006367:HeLa Cells; D006657:Histones / Q000235:genetics / Q000378:metabolism*; D054884:Host-Pathogen Interactions; D006801:Humans; D008089:Listeria monocytogenes / Q000235:genetics / Q000472:pathogenicity*; D008088:Listeriosis / Q000235:genetics* / Q000378:metabolism* / Q000382:microbiology; D008239:Lysine / Q000235:genetics / Q000378:metabolism; D008565:Membrane Proteins / Q000235:genetics; D051379:Mice; D008807:Mice, Inbred BALB C; D018345:Mice, Knockout; D011499:Protein Processing, Post-Translational; D019859:Proto-Oncogene Proteins c-met / Q000378:metabolism; D056565:Sirtuin 2 / Q000235:genetics / Q000378:metabolism*; D024363:Transcription Initiation Site; D015533:Transcriptional Activation
chemical_list: D001426:Bacterial Proteins; D002843:Chromatin; D006657:Histones; D008565:Membrane Proteins; C095768:inlB protein, Listeria monocytogenes; D019859:Proto-Oncogene Proteins c-met; C474199:SIRT2 protein, human; C418743:Sirt2 protein, mouse; D056565:Sirtuin 2; D008239:Lysine