title: Methods for analysis of disease resistance and the defense response in Arabidopsis.
abstract: Many G proteins are involved in the plant defense responses against pathogen infection. With Arabidopsis as a model, this chapter describes the protocols commonly used for inoculating plants with Pseudomonas syringae (a bacterial pathogen) and Botrytis cinerea (a fungal pathogen) for analyzing disease resistance phenotypes caused by these pathogens. In addition, the procedures are provided for observation of the hypersensitive response triggered by avirulent strains of P. syringae and for analyzing pathogen-responsive gene expression and MAPK activation.
authors.affiliation: Department of Biology, Hong Kong Baptist University, Kowloon Tong, Kowloon, Hong Kong.
authors.forename: Guojing
authors.initials: G
authors.lastname: Li
authors.forename: Xiujuan
authors.initials: X
authors.lastname: Zhang
authors.forename: Dongli
authors.initials: D
authors.lastname: Wan
authors.forename: Shuqun
authors.initials: S
authors.lastname: Zhang
authors.forename: Yiji
authors.initials: Y
authors.lastname: Xia
mesh_terms: D017360:Arabidopsis / Q000235:genetics*; D020171:Botrytis / Q000472:pathogenicity*; D060467:Disease Resistance / Q000235:genetics*; D019204:GTP-Binding Proteins / Q000235:genetics / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D020929:Mitogen-Activated Protein Kinase Kinases / Q000235:genetics; D010935:Plant Diseases / Q000235:genetics / Q000382:microbiology; D018515:Plant Leaves / Q000235:genetics / Q000382:microbiology; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism; D044224:Pseudomonas syringae / Q000235:genetics / Q000472:pathogenicity*
chemical_list: D020929:Mitogen-Activated Protein Kinase Kinases; D019204:GTP-Binding Proteins