title: Trichloroethylene aerobic cometabolism by suspended and immobilized butane-growing microbial consortia: a kinetic study.
abstract: A kinetic study of butane uptake and trichloroethylene (TCE) aerobic cometabolism was conducted by two suspended-cell (15 and 30°C) and two attached-cell (15 and 30°C) consortia obtained from the indigenous biomass of a TCE-contaminated aquifer. The shift from suspended to attached cells resulted in an increase of butane (15 and 30°C) and TCE (15°C) biodegradation rates, and a significant decrease of butane inhibition on TCE biodegradation. The TCE 15°C maximum specific biodegradation rate was equal to 0.011 mg(TCE ) mg(protein)(-1) d(-1) with suspended cells and 0.021 mg(TCE) mg(protein)(-1) d(-1) with attached cells. The type of mutual butane/TCE inhibition depended on temperature and biomass conditions. On the basis of a continuous-flow simulation, a packed-bed PFR inoculated with the 15 or 30°C attached-cell consortium could attain a 99.96% conversion of the studied site's average TCE concentration with a 0.4-0.5-day hydraulic residence time, with a low effect of temperature on the TCE degradation performances.
authors.affiliation: Department of Civil, Chemical, Environmental and Materials Engineering, University of Bologna, Bologna, Italy. dario.frascari@unibo.it
authors.forename: Dario
authors.initials: D
authors.lastname: Frascari
authors.forename: Giulio
authors.initials: G
authors.lastname: Zanaroli
authors.forename: Giacomo
authors.initials: G
authors.lastname: Bucchi
authors.forename: Antonella
authors.initials: A
authors.lastname: Rosato
authors.forename: Nasrin
authors.initials: N
authors.lastname: Tavanaie
authors.forename: Serena
authors.initials: S
authors.lastname: Fraraccio
authors.forename: Davide
authors.initials: D
authors.lastname: Pinelli
authors.forename: Fabio
authors.initials: F
authors.lastname: Fava
mesh_terms: D000332:Aerobiosis; D001673:Biodegradation, Environmental; D019149:Bioreactors / Q000382:microbiology; D001711:Biotransformation; D002073:Butanes / Q000378:metabolism*; D018914:Cells, Immobilized / Q000378:metabolism; D003198:Computer Simulation; D007700:Kinetics; D059013:Microbial Consortia; D014241:Trichloroethylene / Q000378:metabolism*
chemical_list: D002073:Butanes; D014241:Trichloroethylene; C046888:butane
keywords: Aerobic cometabolism; Biofilm; Inhibition; Kinetic study; Trichloroethylene