title: Two mammalian MAGOH genes contribute to exon junction complex composition and nonsense-mediated decay.
abstract: The exon junction complex (EJC) participates in the regulation of many post-transcriptional steps of gene expression. EJCs are deposited on messenger RNAs (mRNAs) during splicing and their core consists of eIF4A3, MLN51, Y14, and MAGOH. Here, we show that two genes encoding MAGOH paralogs (referred to as MAGOH and MAGOHB) are expressed in mammals. In macrophages, the expression of MAGOHB, but not MAGOH mRNA, increases rapidly after LPS stimulation. Both MAGOH proteins interact with other EJC components, incorporate into mRNA-bound EJCs, and activate nonsense-mediated decay. Furthermore, the simultaneous depletion of MAGOH and MAGOHB, but not individual depletions, impair nonsense-mediated decay in human cells. Hence, our results establish that the core composition of mammalian EJCs is more complex than previously recognized.
authors.affiliation: University of Cologne; Institute for Genetics; Cologne, Germany.
authors.forename: Kusum K
authors.initials: KK
authors.lastname: Singh
authors.forename: Laurens
authors.initials: L
authors.lastname: Wachsmuth
authors.forename: Andreas E
authors.initials: AE
authors.lastname: Kulozik
authors.forename: Niels H
authors.initials: NH
authors.lastname: Gehring
mesh_terms: D000818:Animals; D019143:Evolution, Molecular; D005091:Exons; D006367:HeLa Cells; D006801:Humans; D008264:Macrophages / Q000378:metabolism; D051379:Mice; D059365:Nonsense Mediated mRNA Decay / Q000235:genetics*; D009687:Nuclear Proteins / Q000235:genetics* / Q000378:metabolism*; D012326:RNA Splicing; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000378:metabolism; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
chemical_list: C491494:MAGOH protein, human; C491493:Magoh protein, mouse; D009687:Nuclear Proteins; D012333:RNA, Messenger; D016601:RNA-Binding Proteins
keywords: EJC; MAGOHB; NMD; gene duplication; mago nashi homolog
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