title: Presymptomatic prediction of preeclampsia with angiogenic factors, in high risk pregnant women.
abstract: BACKGROUND
  The aim of this study was to investigate the value of placental growth factor (PLGF), soluble fms-like tyrosine kinase-1 (sFlt-1) and sFlt-1/PLGF ratio, in predicting symptomatic preeclampsia (PE).
  
  
  METHODS
  A prospective longitudinal study was carried out on 71 high risk preeclamptic women cohort. All of them had normal blood pressure level (≤140/90 mmHg) at the time of enrolment, 26.8 ± 1.5 weeks. Maternal blood was collected and plasma was stored in a freezer at -80 °C. PE was defined according to the National High Blood Pressure Education Program Working Group Criteria. Accuracy of angiogenic factors in predicting PE was evaluated using Receiver-operating characteristics.
  
  
  RESULTS
  Maternal plasma concentrations of PLGF and sFlt-1 were able to predict PE (0.90, p < 001; 0.78, p = 0.003, area under the curve, respectively) but the sFlt-1/PLGF ratio presented the best prediction potential over the others (0.95, area under the curve, p < 0.001).
  
  
  CONCLUSIONS
  All angiogenesis factors were effective biomarkers in predicting PE during the second trimester, before the clinical onset of PE.
authors.affiliation: Fetal Medicine Center, Obstetrics and Gynecology Department, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil. patigt@terra.com.br
authors.forename: Patrícia G
authors.initials: PG
authors.lastname: Teixeira
authors.forename: Zilma S N
authors.initials: ZS
authors.lastname: Reis
authors.forename: Silvia P
authors.initials: SP
authors.lastname: Andrade
authors.forename: Cézar A
authors.initials: CA
authors.lastname: Rezende
authors.forename: Eura M
authors.initials: EM
authors.lastname: Lage
authors.forename: Elizabeth P
authors.initials: EP
authors.lastname: Velloso
authors.forename: Carolina A S
authors.initials: CA
authors.lastname: Santana
authors.forename: Antônio C V
authors.initials: AC
authors.lastname: Cabral
mesh_terms: D000293:Adolescent; D000328:Adult; D015415:Biomarkers / Q000097:blood; D005260:Female; D006801:Humans; D008137:Longitudinal Studies; D000072483:Placenta Growth Factor; D011225:Pre-Eclampsia / Q000097:blood*; D011237:Predictive Value of Tests; D011247:Pregnancy; D011257:Pregnancy Proteins / Q000097:blood*; D011262:Pregnancy Trimester, Second; D011446:Prospective Studies; D040281:Vascular Endothelial Growth Factor Receptor-1 / Q000097:blood*; D055815:Young Adult
chemical_list: D015415:Biomarkers; C000608036:PGF protein, human; D011257:Pregnancy Proteins; D000072483:Placenta Growth Factor; C501162:FLT1 protein, human; D040281:Vascular Endothelial Growth Factor Receptor-1