title: Albumin-conjugated pH/thermo responsive poly(amino urethane) multiblock copolymer as an injectable hydrogel for protein delivery.
abstract: Albumin-conjugated pH and thermo responsive poly(amino urethane) multiblock copolymers are synthesized. The aqueous solution of the conjugate exhibits pH/temperature dependent sol-gel phase transitions that can be tuned by changing solution concentration and albumin composition. The gel window well covers the physiological condition allowing the flowing liquid to readily form the solid gel under the back of the Sprangue-Dawley (SD) rats. The release profile of lysozyme, as a model protein, shows that the conjugate hydrogel is able to effectively reduce the burst effect, in vivo, and maintain the sustained release of lysozyme over 4 and 2 weeks in the in vitro and in vivo experiments, respectively. Such conjugate hydrogel can be considered as a potential candidate for long-term delivery of proteins.
authors.affiliation: Faculty of Science, Department of Chemistry and Materials Science Research Center, Chiang Mai University, Chiang Mai, 50200, Thailand.
authors.forename: Kiattikhun
authors.initials: K
authors.lastname: Manokruang
authors.forename: Doo Sung
authors.initials: DS
authors.lastname: Lee
mesh_terms: D000418:Albumins / Q000737:chemistry*; D000818:Animals; D002417:Cattle; D002645:Chickens; D016503:Drug Delivery Systems; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007267:Injections; D009113:Muramidase / Q000097:blood / Q000378:metabolism*; D044367:Phase Transition; D011092:Polyethylene Glycols / Q000138:chemical synthesis* / Q000737:chemistry; D011140:Polyurethanes / Q000138:chemical synthesis* / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D012710:Serum Albumin, Bovine / Q000378:metabolism; D012996:Solutions; D017550:Spectroscopy, Fourier Transform Infrared; D055672:Static Electricity; D013696:Temperature
chemical_list: D000418:Albumins; D011140:Polyurethanes; D012996:Solutions; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D012710:Serum Albumin, Bovine; D011092:Polyethylene Glycols; D009113:Muramidase
keywords: injectable hydrogels; polymer-protein conjugates; polyurethanes; stimuli responsive polymers