title: Fabrication of DNA microarrays on polydopamine-modified gold thin films for SPR imaging measurements.
abstract: Polydopamine (PDA) films were fabricated on thin film gold substrates in a single-step polymerization-deposition process from dopamine solutions and then employed in the construction of robust DNA microarrays for the ultrasensitive detection of biomolecules with nanoparticle-enhanced surface plasmon resonance (SPR) imaging. PDA multilayers with thicknesses varying from 1 to 5 nm were characterized with a combination of scanning angle SPR and AFM experiments, and 1.3 ± 0.2 nm PDA multilayers were chosen as an optimal thickness for the SPR imaging measurements. DNA microarrays were then fabricated by the reaction of amine-functionalized single-stranded DNA (ssDNA) oligonucleotides with PDA-modified gold thin film microarray elements, and were subsequently employed in SPR imaging measurements of DNA hybridization adsorption and protein-DNA binding. Concurrent control experiments with non-complementary ssDNA sequences demonstrated that the adhesive PDA multilayer was also able to provide good resistance to the nonspecific binding of biomolecules. Finally, a series of SPR imaging measurements of the hybridization adsorption of DNA-modified gold nanoparticles onto mixed sequence DNA microarrays were used to confirm that the use of PDA multilayer films is a simple, rapid, and versatile method for fabricating DNA microarrays for ultrasensitive nanoparticle-enhanced SPR imaging biosensing.
authors.affiliation: Department of Chemistry, University of California-Irvine, Irvine, California 92697, United States.
authors.forename: Jennifer B
authors.initials: JB
authors.lastname: Wood
authors.forename: Megan W
authors.initials: MW
authors.lastname: Szyndler
authors.forename: Aaron R
authors.initials: AR
authors.lastname: Halpern
authors.forename: Kyunghee
authors.initials: K
authors.lastname: Cho
authors.forename: Robert M
authors.initials: RM
authors.lastname: Corn
mesh_terms: D006046:Gold / Q000737:chemistry*; D007211:Indoles / Q000737:chemistry*; D008567:Membranes, Artificial; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D011108:Polymers / Q000737:chemistry*; D020349:Surface Plasmon Resonance
chemical_list: D007211:Indoles; D008567:Membranes, Artificial; D011108:Polymers; C568283:polydopamine; D006046:Gold
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