title: Technical aspects of the mouse aortocaval fistula.
abstract: Technical aspects of creating an arteriovenous fistula in the mouse are discussed. Under general anesthesia, an abdominal incision is made, and the aorta and inferior vena cava (IVC) are exposed. The proximal infrarenal aorta and the distal aorta are dissected for clamp placement and needle puncture, respectively. Special attention is paid to avoid dissection between the aorta and the IVC. After clamping the aorta, a 25 G needle is used to puncture both walls of the aorta into the IVC. The surrounding connective tissue is used for hemostatic compression. Successful creation of the AVF will show pulsatile arterial blood flow in the IVC. Further confirmation of successful AVF can be achieved by post-operative Doppler ultrasound.
authors.affiliation: Department of Surgery and the Interdepartmental Program in Vascular Biology and Therapeutics, Yale University, USA.
authors.forename: Kota
authors.initials: K
authors.lastname: Yamamoto
authors.forename: Xin
authors.initials: X
authors.lastname: Li
authors.forename: Chang
authors.initials: C
authors.lastname: Shu
authors.forename: Tetsuro
authors.initials: T
authors.lastname: Miyata
authors.forename: Alan
authors.initials: A
authors.lastname: Dardik
mesh_terms: D000818:Animals; D001164:Arteriovenous Fistula; D001166:Arteriovenous Shunt, Surgical / Q000379:methods*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL
references.citation: Circulation. 2001 Feb 20;103(7):1012-6
references.pmid: 11181478
references.citation: Am J Pathol. 2003 Jun;162(6):2079-90
references.pmid: 12759262
references.citation: Kidney Int. 2006 Jul;70(2):315-20
references.pmid: 16760906
references.citation: J Invest Surg. 2005 Nov-Dec;18(6):325-34
references.pmid: 16319054
references.citation: Surg Neurol. 2007 Sep;68(3):277-84; discussion 284
references.pmid: 17719966
references.citation: Arterioscler Thromb Vasc Biol. 2007 Jul;27(7):1562-71
references.pmid: 17463332
references.citation: Clin J Am Soc Nephrol. 2007 Jul;2(4):786-800
references.pmid: 17699495
references.citation: Am J Physiol Renal Physiol. 2007 Aug;293(2):F470-5
references.pmid: 17344190
references.citation: J Exp Med. 2011 Mar 14;208(3):561-75
references.pmid: 21339325
references.citation: Am J Pathol. 2010 May;176(5):2530-41
references.pmid: 20363917
references.citation: J Nephrol. 2007 Mar-Apr;20(2):150-63
references.pmid: 17514619
references.citation: Kidney Int. 2006 Oct;70(8):1413-22
references.pmid: 16883317
references.citation: J Vasc Interv Radiol. 2008 Apr;19(4):587-94
references.pmid: 18375305
references.citation: J Surg Res. 2002 Nov;108(1):112-21
references.pmid: 12443723