title: DNA methylation of dermal MSCs in psoriasis: identification of epigenetically dysregulated genes.
abstract: BACKGROUND
  Mesenchymal stem cells (MSCs) are likely involved in pathological processes of immune-related diseases, including psoriasis, because of their immunoregulatory and pro-angiogenic effects. DNA methylation plays an essential role in regulating gene expression and maintaining cell function.
  
  
  OBJECTIVE
  This study aimed to investigate the gene methylation profile of dermal MSCs from patients with psoriasis.
  
  
  METHODS
  We isolated and expanded dermal MSCs from psoriatic patients and normal controls using the attachment assay and conducted genome-wide DNA methylation profile and gene ontology analyses using microarray.
  
  
  RESULTS
  The cultured cells were indentified as MSCs by surface marker and differentiation assays. The genome-wide promoter methylation profile of MSCs from psoriatic derma was markedly different from the normal derma derived MSCs. Genes involved in cell communication, surface receptor signaling pathway, cellular response to stimulus, and cell migration were differently methylated. Several aberrantly methylated genes related epidermal proliferation, angiogenesis, and inflammation were found differently expressed in psoriatic patients.
  
  
  CONCLUSIONS
  These results indicated that the MSCs from dermal of psoriasis are probably participant in the pathogenesis and development of psoriasis through an extraordinarily complex mechanism.
authors.affiliation: Institute of Dermatology, Taiyuan City Central Hospital, 1 Dong San Dao Xiang, Taiyuan 030009, Shanxi Province, China.
authors.forename: Ruixia
authors.initials: R
authors.lastname: Hou
authors.forename: Guohua
authors.initials: G
authors.lastname: Yin
authors.forename: Peng
authors.initials: P
authors.lastname: An
authors.forename: Chunfang
authors.initials: C
authors.lastname: Wang
authors.forename: Ruifeng
authors.initials: R
authors.lastname: Liu
authors.forename: Yuanwen
authors.initials: Y
authors.lastname: Yang
authors.forename: Xin
authors.initials: X
authors.lastname: Yan
authors.forename: Junqin
authors.initials: J
authors.lastname: Li
authors.forename: Xinhua
authors.initials: X
authors.lastname: Li
authors.forename: Kaiming
authors.initials: K
authors.lastname: Zhang
mesh_terms: D017667:Adipocytes / Q000166:cytology; D000293:Adolescent; D000328:Adult; D002454:Cell Differentiation; D002462:Cell Membrane / Q000378:metabolism; D049109:Cell Proliferation; D019175:DNA Methylation; D044127:Epigenesis, Genetic; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D007107:Immune System; D007249:Inflammation; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D008875:Middle Aged; D009389:Neovascularization, Pathologic; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis; D010006:Osteoblasts / Q000166:cytology; D011401:Promoter Regions, Genetic; D011565:Psoriasis / Q000378:metabolism*; D012867:Skin / Q000378:metabolism; D055815:Young Adult
keywords: Dermal tissue; Gene dysfunction; Mesenchymal stem cells; Microarray; Promoter methylation; Psoriasis