title: Astragalus polysaccharide reduces inflammatory response by decreasing permeability of LPS-infected Caco2 cells.
abstract: As the major constituent of Radix Astragali, Astragalus polysaccharide (APS) is known for its anti-inflammation and immunomodulatory functions. The objective of this study was to investigate the effect of APS on inflammatory response and structural changes in lipopolysaccharide (LPS)-infected Caco2 cells. Caco2 cells were co-cultured with APS and LPS, with APS added after the addition of LPS (post-addition), before the addition of LPS (pre-addition), or simultaneously with the addition of LPS (simultaneous addition). The mRNA expression of inflammatory indicators and tight junctions was measured by RT-qPCR. Short circuit current (Isc) was recorded by an Ussing chamber system. Addition of APS significantly down-regulated the expression of TNF-α, IL-1β and IL-8 (P<0.05) and the Isc levels (P<0.05) of LPS-infected Caco2 cells for all three administration treatments. The minimum anti-inflammatory concentration of APS was 50, 100, and 100 μg/mL for pre-, post-, and simultaneous additions of APS, respectively. The mRNA expression of zonula occludens-1 (ZO-1) and occludin was significantly up-regulated for post- and pre-additions of APS, respectively (P<0.05). Results suggested that APS had anti-inflammatory and structure protective properties for LPS-infected Caco2 cells, and may be used as a preventative treatment for intestine cells.
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, 712100, China.
authors.forename: Xiaofei
authors.initials: X
authors.lastname: Wang
authors.forename: Yulong
authors.initials: Y
authors.lastname: Li
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Yang
authors.forename: Junhu
authors.initials: J
authors.lastname: Yao
mesh_terms: D000893:Anti-Inflammatory Agents / Q000494:pharmacology* / Q000633:toxicity; D027883:Astragalus Plant / Q000737:chemistry*; D018938:Caco-2 Cells; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D008070:Lipopolysaccharides; D062793:Occludin / Q000235:genetics / Q000378:metabolism; D010539:Permeability / Q000187:drug effects; D011134:Polysaccharides / Q000494:pharmacology* / Q000633:toxicity; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D062826:Zonula Occludens-1 Protein / Q000235:genetics / Q000378:metabolism
chemical_list: D000893:Anti-Inflammatory Agents; D008070:Lipopolysaccharides; D062793:Occludin; D011134:Polysaccharides; D012333:RNA, Messenger; D062826:Zonula Occludens-1 Protein
keywords: Astragalus polysaccharide; Inflammatory response; Lipopolysaccharide