title: Activated platelet-rich plasma improves adipose-derived stem cell transplantation efficiency in injured articular cartilage.
abstract: BACKGROUND
  Adipose-derived stem cells (ADSCs) have been isolated, expanded, and applied in the treatment of many diseases. ADSCs have also been used to treat injured articular cartilage. However, there is controversy regarding the treatment efficiency. We considered that ADSC transplantation with activated platelet-rich plasma (PRP) may improve injured articular cartilage compared with that of ADSC transplantation alone. In this study, we determined the role of PRP in ADSC transplantation to improve the treatment efficiency.
  
  
  METHODS
  ADSCs were isolated and expanded from human adipose tissue. PRP was collected and activated from human peripheral blood. The effects of PRP were evaluated in vitro and in ADSC transplantation in vivo. In vitro, the effects of PRP on ADSC proliferation, differentiation into chondrogenic cells, and inhibition of angiogenic factors were investigated at three concentrations of PRP (10%, 15% and 20%). In vivo, ADSCs pretreated with or without PRP were transplanted into murine models of injured articular cartilage.
  
  
  RESULTS
  PRP promoted ADSC proliferation and differentiation into chondrogenic cells that strongly expressed collagen II, Sox9 and aggrecan. Moreover, PRP inhibited expression of the angiogenic factor vascular endothelial growth factor. As a result, PRP-pretreated ADSCs improved healing of injured articular cartilage in murine models compared with that of untreated ADSCs.
  
  
  CONCLUSIONS
  Pretreatment of ADSCs with PRP is a simple method to efficiently apply ADSCs in cartilage regeneration. This study provides an important step toward the use of autologous ADSCs in the treatment of injured articular cartilage.
authors.forename: Phuc
authors.initials: P
authors.lastname: Van Pham
authors.forename: Khanh Hong-Thien
authors.initials: KH
authors.lastname: Bui
authors.forename: Dat Quoc
authors.initials: DQ
authors.lastname: Ngo
authors.forename: Ngoc Bich
authors.initials: NB
authors.lastname: Vu
authors.forename: Nhung Hai
authors.initials: NH
authors.lastname: Truong
authors.forename: Nhan Lu-Chinh
authors.initials: NL
authors.lastname: Phan
authors.forename: Dung Minh
authors.initials: DM
authors.lastname: Le
authors.forename: Triet Dinh
authors.initials: TD
authors.lastname: Duong
authors.forename: Thanh Duc
authors.initials: TD
authors.lastname: Nguyen
authors.forename: Vien Tuong
authors.initials: VT
authors.lastname: Le
authors.forename: Ngoc Kim
authors.initials: NK
authors.lastname: Phan
mesh_terms: D000273:Adipose Tissue / Q000166:cytology / Q000378:metabolism*; D000818:Animals; D002358:Cartilage, Articular; D002454:Cell Differentiation; D006801:Humans; D016130:Immunophenotyping; D051379:Mice; D053657:Platelet-Rich Plasma; D012038:Regeneration; D033581:Stem Cell Transplantation; D023822:Tissue Engineering / Q000379:methods*
references.citation: Tohoku J Exp Med. 2012;226(3):231-41
references.pmid: 22450704
references.citation: Am J Pathol. 2003 Jan;162(1):171-81
references.pmid: 12507900
references.citation: Biomaterials. 2012 Oct;33(29):7008-18
references.pmid: 22818985
references.citation: Stem Cells. 2007 May;25(5):1270-8
references.pmid: 17255520
references.citation: Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2009 Jun;23(6):732-6
references.pmid: 19594024
references.citation: J Med Eng. 2013;2013:832396
references.pmid: 27006923
references.citation: Cell Transplant. 2009;18(2):145-58
references.pmid: 19499703
references.citation: J Bone Joint Surg Am. 2012 Apr 4;94(7):609-17
references.pmid: 22488617
references.citation: Transfusion. 2012 Dec;52(12):2551-8
references.pmid: 22486546
references.citation: Ann Rheum Dis. 2001 Nov;60(11):1070-3
references.pmid: 11602483
references.citation: Cell Transplant. 2012;21(12):2753-64
references.pmid: 22776464
references.citation: Nan Fang Yi Ke Da Xue Xue Bao. 2011 Mar;31(3):525-8
references.pmid: 21421498
references.citation: Cytotherapy. 2011 Sep;13(8):933-43
references.pmid: 21623669
references.citation: Int J Mol Med. 2011 Oct;28(4):565-72
references.pmid: 21687931
references.citation: Int J Mol Med. 2012 Jan;29(1):32-6
references.pmid: 21964487
references.citation: Biomaterials. 2012 Mar;33(7):2016-24
references.pmid: 22189147
references.citation: Stem Cell Res Ther. 2012 Aug 24;3(4):35
references.pmid: 22920724
references.citation: J Card Fail. 2010 Apr;16(4):357-66
references.pmid: 20350704
references.citation: Cell Transplant. 2012;21(12):2723-33
references.pmid: 22524986
references.citation: Knee Surg Sports Traumatol Arthrosc. 2014 Jun;22(6):1225-34
references.pmid: 23563814
references.citation: J Orthop Res. 2009 Dec;27(12):1675-80
references.pmid: 19544397
references.citation: Curr Stem Cell Res Ther. 2012 Sep;7(5):347-55
references.pmid: 22563658
references.citation: Vet Ther. 2007 Winter;8(4):272-84
references.pmid: 18183546
references.citation: J Med Case Rep. 2011 Jul 07;5:296
references.pmid: 21736710
references.citation: Tissue Eng. 2001 Apr;7(2):211-28
references.pmid: 11304456
references.citation: Cell Prolif. 2012 Apr;45(2):111-20
references.pmid: 22260253
references.citation: Eur J Orthop Surg Traumatol. 2013 Jul;23(5):581-7
references.pmid: 23412171
references.citation: J Bone Joint Surg Am. 2012 Dec 5;94(23):e1721-7
references.pmid: 23224392
references.citation: Am J Vet Res. 2007 Sep;68(9):941-5
references.pmid: 17764407
references.citation: Arthritis Rheum. 2012 Nov;64(11):3604-13
references.pmid: 22961401
references.citation: Am J Phys Med Rehabil. 2012 May;91(5):411-7
references.pmid: 22513879
references.citation: Osteoarthritis Cartilage. 2013 Jan;21(1):175-85
references.pmid: 23041435
references.citation: Int J Neurosci. 2011 Apr;121(4):176-80
references.pmid: 21244302
references.citation: Plast Reconstr Surg. 2008 Nov;122(5):1352-1360
references.pmid: 18971718
references.citation: Cytometry A. 2013 Jan;83(1):134-40
references.pmid: 23184564
references.citation: Vet Ther. 2008 Fall;9(3):192-200
references.pmid: 19003780
references.citation: Cytotherapy. 2006;8(4):315-7
references.pmid: 16923606
references.citation: Knee. 2011 Mar;18(2):71-5
references.pmid: 20591677
references.citation: Biochem Biophys Res Commun. 2012 Oct 5;426(4):544-50
references.pmid: 22982316
references.citation: Arthroscopy. 2013 Apr;29(4):748-55
references.pmid: 23375182
references.citation: Tissue Eng. 2004 Mar-Apr;10(3-4):575-84
references.pmid: 15165474
references.citation: Ann Dermatol. 2011 Nov;23(4):424-31
references.pmid: 22148008
references.citation: Arthritis Rheum. 2003 Dec;48(12):3464-74
references.pmid: 14673997
references.citation: Ann Rehabil Med. 2012 Aug;36(4):458-65
references.pmid: 22977770
references.citation: Eur J Orthop Surg Traumatol. 2013 Jul;23(5):573-80
references.pmid: 23412170
references.citation: Clin Lab. 2012;58(9-10):897-903
references.pmid: 23163104
references.citation: Osteoarthritis Cartilage. 2001 Feb;9(2):112-8
references.pmid: 11237658
references.citation: Am J Sports Med. 2013 Feb;41(2):356-64
references.pmid: 23299850
references.citation: J Mech Behav Biomed Mater. 2012 Jul;11:102-11
references.pmid: 22658159
references.citation: Cell Transplant. 2013;22(5):779-95
references.pmid: 22526170
references.citation: Stem Cells Transl Med. 2012 Nov;1(11):771-82
references.pmid: 23197689
references.citation: J Transl Med. 2009 Apr 24;7:29
references.pmid: 19393041
references.citation: Biotechnol Appl Biochem. 2011 Jul-Aug;58(4):271-6
references.pmid: 21838802
references.citation: J Pathol. 2004 Mar;202(3):367-74
references.pmid: 14991903
references.citation: Biochem Biophys Res Commun. 2012 Jul 27;424(2):234-8
references.pmid: 22728881
references.citation: Knee. 2012 Dec;19(6):902-7
references.pmid: 22583627
references.citation: Cell Biol Int. 2012 Feb;36(2):189-94
references.pmid: 21936851
references.citation: Stem Cells Int. 2013;2013:246134
references.pmid: 23533440
references.citation: Acta Cir Bras. 2012 Jul;27(7):471-6
references.pmid: 22760832
references.citation: Biomacromolecules. 2008 Mar;9(3):927-34
references.pmid: 18269244
references.citation: In Vitro Cell Dev Biol Anim. 2011 Jan;47(1):54-63
references.pmid: 21082287
references.citation: Am J Phys Med Rehabil. 2010 Oct;89(10):854-61
references.pmid: 20855985
references.citation: Arthritis Rheum. 2011 Nov;63(11):3344-53
references.pmid: 21769848