title: Fluorescence detection of cationic amyloid fibrils in human semen.
abstract: Cationic amyloid fibrils, including the Semen Enhancer of Virus Infection (SEVI), have recently been described in human semen. Simple methods for quantitating these fibrils are needed to improve our understanding of their biological function. We performed high-throughput screening to identify molecules that bind SEVI, and identified a small molecule (8E2), that fluoresced brightly in the presence of SEVI and other cationic fibrils. 8E2 bound SEVI with almost 40-fold greater affinity than thioflavin-T, and could efficiently detect high molecular weight fibrils in human seminal fluid.
authors.affiliation: Department of Microbiology and Immunology, University of Rochester, Rochester, NY 14642, USA.
authors.forename: David
authors.initials: D
authors.lastname: Easterhoff
authors.forename: John T M
authors.initials: JT
authors.lastname: DiMaio
authors.forename: Wathsala
authors.initials: W
authors.lastname: Liyanage
authors.forename: Chi-Wen
authors.initials: CW
authors.lastname: Lo
authors.forename: Woori
authors.initials: W
authors.lastname: Bae
authors.forename: Todd M
authors.initials: TM
authors.lastname: Doran
authors.forename: Alan
authors.initials: A
authors.lastname: Smrcka
authors.forename: Bradley L
authors.initials: BL
authors.lastname: Nilsson
authors.forename: Stephen
authors.initials: S
authors.lastname: Dewhurst
mesh_terms: D000682:Amyloid / Q000032:analysis*; D002412:Cations / Q000032:analysis; D006801:Humans; D015394:Molecular Structure; D012661:Semen / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
chemical_list: D000682:Amyloid; D002412:Cations
keywords: Amyloid; Fluorescence; SEVI; Semen; Thioflavin T
references.citation: J Virol. 2012 Jan;86(2):1244-9
references.pmid: 22090109
references.citation: J Am Chem Soc. 2012 Oct 24;134(42):17338-41
references.pmid: 22866977
references.citation: J Phys Chem B. 2008 Dec 11;112(49):15893-902
references.pmid: 19367903
references.citation: Cell. 2007 Dec 14;131(6):1059-71
references.pmid: 18083097
references.citation: J Struct Biol. 2000 Jun;130(2-3):88-98
references.pmid: 10940217
references.citation: Biochim Biophys Acta. 2011 Apr;1808(4):1161-9
references.pmid: 21262195
references.citation: J Struct Biol. 2008 Jun;162(3):387-96
references.pmid: 18406172
references.citation: Proc Natl Acad Sci U S A. 2009 Jun 2;106(22):9033-8
references.pmid: 19451623
references.citation: PeerJ. 2013 Feb 12;1:e5
references.pmid: 23638387
references.citation: ACS Chem Neurosci. 2011 May 18;2(5):249-255
references.pmid: 21743829
references.citation: Retrovirology. 2010 Jun 23;7:55
references.pmid: 20573198
references.citation: J Am Chem Soc. 2013 May 22;135(20):7503-10
references.pmid: 23611538
references.citation: Nat Struct Mol Biol. 2008 Jun;15(6):558-66
references.pmid: 18511942
references.citation: J Phys Chem B. 2012 Mar 22;116(11):3650-8
references.pmid: 22360607
references.citation: Biochemistry. 2012 Oct 16;51(41):8154-62
references.pmid: 22998665
references.citation: J Virol. 2009 Jan;83(1):73-80
references.pmid: 18945786
references.citation: Cell Host Microbe. 2011 Dec 15;10(6):541-50
references.pmid: 22177559
references.citation: Anal Biochem. 1989 Mar;177(2):244-9
references.pmid: 2729542
references.citation: Biophys J. 2011 Mar 2;100(5):1325-34
references.pmid: 21354406
references.citation: J Mol Biol. 2009 Jan 30;385(4):1052-63
references.pmid: 19038267