title: Characterization and identification of PARM-1 as a new potential oncogene.
abstract: BACKGROUND
  The Graffi murine retrovirus is a powerful tool to find leukemia associated oncogenes. Using DNA microarrays, we recently identified several genes specifically deregulated in T- and B-leukemias induced by this virus.
  
  
  RESULTS
  In the present study, probsets associated with T-CD8+ leukemias were analyzed and we validated the expression profile of the Parm-1 gene. PARM-1 is a member of the mucin family. We showed that human PARM-1 is an intact secreted protein accumulating predominantly, such as murine PARM-1, at the Golgi and in the early and late endosomes. PARM-1 colocalization with α-tubulin suggests that its trafficking within the cell involves the microtubule cytoskeleton. Also, the protein co-localizes with caveolin-1 which probably mediates its internalization. Transient transfection of both mouse and human Parm-1 cDNAs conferred anchorage- and serum-independent growth and enhanced cell proliferation. Moreover, deletion mutants of human PARM-1 without either extracellular or cytoplasmic portions seem to retain the ability to induce anchorage-independent growth of NIH/3T3 cells. In addition, PARM-1 increases ERK1/2, but more importantly AKT and STAT3 phosphorylation.
  
  
  CONCLUSIONS
  Our results strongly suggest the oncogenic potential of PARM-1.
authors.affiliation: Laboratoire de Biologie Moléculaire, Département des Sciences Biologiques, Centre BioMed, Université du Québec à Montréal, Case Postale 8888, Succursale Centre-ville, Montréal, QC H3C-3P8, Canada.
authors.forename: Cyndia
authors.initials: C
authors.lastname: Charfi
authors.forename: Louis-Charles
authors.initials: LC
authors.lastname: Levros
authors.forename: Elsy
authors.initials: E
authors.lastname: Edouard
authors.forename: Eric
authors.initials: E
authors.lastname: Rassart
mesh_terms: D000727:Androgen-Binding Protein / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D018414:CD8-Positive T-Lymphocytes / Q000378:metabolism / Q000473:pathology; D051242:Caveolin 1; D049109:Cell Proliferation; D002471:Cell Transformation, Neoplastic / Q000235:genetics; D011992:Endosomes / Q000378:metabolism; D015870:Gene Expression; D020869:Gene Expression Profiling; D006056:Golgi Apparatus / Q000378:metabolism; D006801:Humans; D015458:Leukemia, T-Cell / Q000235:genetics / Q000378:metabolism; D051379:Mice; D019950:Mitogen-Activated Protein Kinase 1 / Q000378:metabolism; D048052:Mitogen-Activated Protein Kinase 3 / Q000378:metabolism; D009154:Mutation; D041681:NIH 3T3 Cells; D009857:Oncogenes; D011485:Protein Binding; D021381:Protein Transport; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D050796:STAT3 Transcription Factor / Q000378:metabolism; D015398:Signal Transduction; D014404:Tubulin / Q000378:metabolism
chemical_list: D000727:Androgen-Binding Protein; D051242:Caveolin 1; D019950:Mitogen-Activated Protein Kinase 1; D048052:Mitogen-Activated Protein Kinase 3; D051057:Proto-Oncogene Proteins c-akt; D050796:STAT3 Transcription Factor; D014404:Tubulin; C527680:PARM1 protein, human
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