title: Proteomic and phytohormone analysis of the response of maize (Zea mays L.) seedlings to sugarcane mosaic virus.
abstract: BACKGROUND
  Sugarcane mosaic virus (SCMV) is an important virus pathogen in crop production, causing serious losses in grain and forage yields in susceptible cultivars. Control strategies have been developed, but only marginal successes have been achieved. For the efficient control of this virus, a better understanding of its interactions and associated resistance mechanisms at the molecular level is required.
  
  
  RESULTS
  The responses of resistant and susceptible genotypes of maize to SCMV and the molecular basis of the resistance were studied using a proteomic approach based on two-dimensional polyacrylamide gel electrophoresis (2-DE) and matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS/MS) analysis. Ninety-six protein spots showed statistically significant differences in intensity after SCMV inoculation. The classification of differentially expressed proteins showed that SCMV-responsive proteins were mainly involved in energy and metabolism, stress and defense responses, and photosynthesis. Most of the proteins identified were located in chloroplasts, chloroplast membranes, and the cytoplasm. Analysis of changes in phytohormone levels after virus inoculation suggested that salicylic acid, abscisic acid, jasmonic acid, and azelaic acid may played important roles in the maize response to SCMV infection.
  
  
  CONCLUSIONS
  Among these identified proteins, 19 have not been identified previously as virus-responsive proteins, and seven were new and did not have assigned functions. These proteins may be candidate proteins for future investigation, and they may present new biological functions and play important roles in plant-virus interactions. The behavioural patterns of the identified proteins suggest the existence of defense mechanisms operating during the early stages of infection that differed in two genotypes. In addition, there are overlapping and specific phytohormone responses to SCMV infection between resistant and susceptible maize genotypes. This study may provide important insights into the molecular events during plant responses to virus infection.
authors.affiliation: Henan Agricultural University and Synergetic Innovation Center of Henan Grain Crops, Zhengzhou, China.
authors.forename: Liuji
authors.initials: L
authors.lastname: Wu
authors.forename: Shunxi
authors.initials: S
authors.lastname: Wang
authors.forename: Xiao
authors.initials: X
authors.lastname: Chen
authors.forename: Xintao
authors.initials: X
authors.lastname: Wang
authors.forename: Liancheng
authors.initials: L
authors.lastname: Wu
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Zu
authors.forename: Yanhui
authors.initials: Y
authors.lastname: Chen
mesh_terms: D060387:Ascorbate Peroxidases / Q000235:genetics / Q000378:metabolism; D015153:Blotting, Western; D060467:Disease Resistance / Q000235:genetics*; D018506:Gene Expression Regulation, Plant; D054464:Peroxiredoxins / Q000235:genetics / Q000378:metabolism; D010641:Phenotype; D010937:Plant Growth Regulators / Q000235:genetics / Q000378:metabolism*; D010940:Plant Proteins / Q000737:chemistry; D017800:Potyvirus / Q000502:physiology*; D021381:Protein Transport; D040901:Proteomics; D036226:Seedlings / Q000821:virology*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism; D003313:Zea mays / Q000235:genetics* / Q000821:virology*
chemical_list: D010937:Plant Growth Regulators; D010940:Plant Proteins; D060387:Ascorbate Peroxidases; D054464:Peroxiredoxins; D013482:Superoxide Dismutase
references.citation: Electrophoresis. 2000 Nov;21(17):3666-72
references.pmid: 11271485
references.citation: Curr Opin Plant Biol. 2005 Aug;8(4):361-8
references.pmid: 15936244
references.citation: Phytochemistry. 2004 Feb;65(3):293-306
references.pmid: 14751300
references.citation: Photosynth Res. 2010 Jan;103(1):31-45
references.pmid: 19823941
references.citation: Proteomics. 2010 Aug;10(16):3051-5
references.pmid: 20662101
references.citation: J Proteome Res. 2007 Sep;6(9):3581-603
references.pmid: 17711327
references.citation: Plant J. 2003 Mar;33(6):975-87
references.pmid: 12631323
references.citation: Trends Plant Sci. 2002 Sep;7(9):405-10
references.pmid: 12234732
references.citation: PLoS Pathog. 2008 Feb 8;4(2):e32
references.pmid: 18282095
references.citation: PLoS One. 2011;6(7):e22255
references.pmid: 21811579
references.citation: Proc Natl Acad Sci U S A. 2004 Jun 29;101(26):9927-32
references.pmid: 15210989
references.citation: Plant J. 2011 Jul;67(1):105-18
references.pmid: 21426425
references.citation: BMB Rep. 2008 Sep 30;41(9):645-50
references.pmid: 18823588
references.citation: Proc Natl Acad Sci U S A. 2010 Feb 2;107(5):2343-8
references.pmid: 20133878
references.citation: Science. 2009 Nov 20;326(5956):1112-5
references.pmid: 19965430
references.citation: Proteomics. 2008 Nov;8(22):4791-807
references.pmid: 18924108
references.citation: Plant Physiol. 2012 Sep;160(1):365-78
references.pmid: 22822212
references.citation: Nature. 1998 Jan 29;391(6666):485-8
references.pmid: 9461215
references.citation: Oncogene. 2006 Feb 2;25(5):713-21
references.pmid: 16186798
references.citation: Trends Microbiol. 1999 Jun;7(6):237-41
references.pmid: 10366860
references.citation: Plant Physiol. 2005 Jul;138(3):1481-90
references.pmid: 15937273
references.citation: Curr Opin Plant Biol. 2002 Aug;5(4):325-31
references.pmid: 12179966
references.citation: Mol Cell Proteomics. 2011 Aug;10(8):M110.006098
references.pmid: 21593212
references.citation: J Virol. 2011 Nov;85(22):11646-54
references.pmid: 21917952
references.citation: Plant J. 2006 Sep;47(6):851-63
references.pmid: 16889645
references.citation: Mol Plant Microbe Interact. 2011 Jan;24(1):7-12
references.pmid: 21138374
references.citation: Gene. 2011 Oct 10;485(2):106-13
references.pmid: 21708230
references.citation: Proc Natl Acad Sci U S A. 2011 Sep 13;108(37):15492-7
references.pmid: 21896747
references.citation: Proteomics. 2008 Sep;8(17):3577-87
references.pmid: 18686304
references.citation: Plant Mol Biol. 2002 Jul;49(5):515-32
references.pmid: 12090627
references.citation: Plant Physiol. 2008 Mar;146(3):839-44
references.pmid: 18316638
references.citation: Proc Natl Acad Sci U S A. 2003 Jun 24;100(13):7824-9
references.pmid: 12810953
references.citation: Biotechnol Adv. 2011 Nov-Dec;29(6):961-71
references.pmid: 21872654
references.citation: Science. 2009 Apr 3;324(5923):89-91
references.pmid: 19342588
references.citation: Phytopathology. 1999 Aug;89(8):660-7
references.pmid: 18944678
references.citation: Plant Mol Biol. 2005 Dec;59(6):981-94
references.pmid: 16307370
references.citation: J Proteome Res. 2009 Jan;8(1):82-93
references.pmid: 18998720
references.citation: BMC Plant Biol. 2009 Feb 02;9:15
references.pmid: 19187556
references.citation: Adv Virus Res. 1987;33:205-55
references.pmid: 3296694
references.citation: Virology. 2006 Jan 5;344(1):169-84
references.pmid: 16364748
references.citation: Annu Rev Plant Physiol Plant Mol Biol. 1996 Jun;47:127-158
references.pmid: 15012285
references.citation: Nature. 2000 Jun 15;405(6788):837-46
references.pmid: 10866210
references.citation: Virology. 1995 Feb 1;206(2):1007-16
references.pmid: 7856075
references.citation: Plant Physiol. 2006 Nov;142(3):901-10
references.pmid: 16950862
references.citation: J Proteome Res. 2010 Nov 5;9(11):5684-97
references.pmid: 20815412
references.citation: Sci Signal. 2009 May 12;2(70):pe31
references.pmid: 19436056
references.citation: J Exp Bot. 2011 Oct;62(14):4763-72
references.pmid: 21705388
references.citation: J Biol Chem. 2012 Jun 15;287(25):21034-44
references.pmid: 22547063
references.citation: Plant Physiol. 2002 Feb;128(2):354-62
references.pmid: 11842139
references.citation: Mol Genet Genomics. 2005 Jul;273(6):450-61
references.pmid: 15891912
references.citation: Proteomics. 2011 May;11(10):1985-2002
references.pmid: 21500340
references.citation: Plant Cell Rep. 2010 Nov;29(11):1261-8
references.pmid: 20734050