title: Determination of neurotransmitter amino acids in mouse central nervous system by CE-LIF.
abstract: An MEKC method with LIF detection has been developed for the determination of seven neurotransmitter amino acids (NAAs) using 1,3,5,7-tetramethyl-8-(N-hydroxysuccinimidyl butyric ester)difluoroboradiaza-S-indacene as the labeling reagent. After derivatization at room temperature for 30 min, the seven target NAAs including glycine, alanine, γ-aminobutyric acid, taurine, glutamine, glutamic acid, and aspartic acid were separated in running buffer, which consisted of 70 mM pH 4.00 H3 PO4 /Na3 PO4 buffer, 5.5 mM cetyltrimethyl ammonium bromide and 20% v/v acetonitrile within 17 min. The LODs were 2 ~ 14 × 10(-10) M without interference from other coexisting amino acids. The proposed method has been applied to the analysis of NAAs in the central nervous systems of healthy mice and those with Alzheimer's disease with recoveries of 92-104%.
authors.affiliation: Hubei Key Laboratory of Economic Forest Germplasm Improvement and Resources Comprehensive Utilization, Huanggang Normal University, Huangzhou, China.
authors.forename: Xu-Jie
authors.initials: XJ
authors.lastname: Xiong
authors.forename: Xiao-Feng
authors.initials: XF
authors.lastname: Guo
authors.forename: Xu-Xia
authors.initials: XX
authors.lastname: Ge
authors.forename: Hong
authors.initials: H
authors.lastname: Wang
authors.forename: Hua-Shan
authors.initials: HS
authors.lastname: Zhang
mesh_terms: D000596:Amino Acids / Q000032:analysis*; D000818:Animals; D002490:Central Nervous System / Q000737:chemistry*; D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods*; D005456:Fluorescent Dyes / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D051379:Mice; D018377:Neurotransmitter Agents / Q000032:analysis*; D013050:Spectrometry, Fluorescence / Q000295:instrumentation / Q000379:methods*
chemical_list: D000596:Amino Acids; D005456:Fluorescent Dyes; D018377:Neurotransmitter Agents
keywords: Central nervous system; Fluorescent probes; MEKC; Neurotransmitter amino acids