title: Nanowire pellicles for eukaryotic cells: nanowire coating and interaction with cells.
abstract: OBJECTIVE
  To construct a new robust nanowire-based pellicle for eukaryotic cells, to investigate the interactions between nanowires (NWs) and cell surfaces and the internalization of nanowires, and to demonstrate for isolation of the plasma membrane with improved enrichment of transmembrane proteins.
  
  
  METHODS
  Silica NWs were coated with alumina to give positive charges on their surface. Multiple myeloma cells were coated with the positively charged NWs by dropping the cells into a buffered suspension of NWs. After the NW-coated cells were lysed, plasma membrane fragments were enriched by differential centrifugation for proteomic studies.
  
  
  RESULTS
  Here we demonstrate complete cell coating with positively charged, alumina-coated silica NWs via nonspecific electrostatic interactions, and characterize a robust pellicle and little/no uptake of NWs.
  
  
  CONCLUSIONS
  Robust pellicles provide a new platform for therapeutic, diagnostic and biochemical interactions of nanostructures with eukaryotic cells.
authors.affiliation: Department of Chemistry & Biochemistry, University of Maryland, College Park, MD 20742, USA.
authors.forename: Sung-Kyoung
authors.initials: SK
authors.lastname: Kim
authors.forename: Waeowalee
authors.initials: W
authors.lastname: Choksawangkarn
authors.forename: Rebecca
authors.initials: R
authors.lastname: Rose
authors.forename: Catherine
authors.initials: C
authors.lastname: Fenselau
authors.forename: Sang Bok
authors.initials: SB
authors.lastname: Lee
mesh_terms: D000327:Adsorption; D000537:Aluminum Oxide / Q000737:chemistry*; D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000737:chemistry; D006801:Humans; D008565:Membrane Proteins / Q000032:analysis*; D009101:Multiple Myeloma / Q000737:chemistry*; D053770:Nanowires / Q000737:chemistry* / Q000648:ultrastructure; D012822:Silicon Dioxide / Q000737:chemistry*
chemical_list: D008565:Membrane Proteins; D012822:Silicon Dioxide; D000537:Aluminum Oxide
keywords: anodic aluminum oxide; cell coating; electrostatic interactions; mass spectrometry; multiple myeloma cells; nanowire; pellicles; plasma membrane; proteomics; transmembrane proteins
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