title: C-type natriuretic peptide transcriptomic profiling increases in human leukocytes of patients with chronic heart failure as a function of clinical severity.
abstract: The aim of this study was to evaluate the transcriptomic profiling of C-type natriuretic peptide (CNP) and of its specific receptor, NPR-B in human leukocytes of heart failure (HF) patients as a function of clinical severity, assessing the possible changes with respect to healthy subjects (C). mRNA expression was evaluated by Real-Time PCR and total RNA was extracted from leukocytes of C (n=8) and of HF patients (NYHA I-II, n=7; NYHA III-IV, n=13) with PAXgene Blood RNA Kit. Significantly higher levels of CNP mRNA expression were found in HF patients as a function of clinical severity (C=0.23±0.058, NYHA I-II=0.47±0.18, NYHA III-IV=2.58±0.71, p=0.005 C vs NYHA III-IV, p=0.017 NYHA I-II vs NYHA III-IV) and NPR-B transcript levels resulted down-regulated in HF patients with higher NYHA class (C=2.2±0.61, NYHA I-II=2.76±0.46, NYHA III-IV=0.29±0.13, p=0.001 C vs NYHA III-IV, p<0.0001 NYHA I-II vs NYHA III-IV). A significant negative correlation between CNP and NPR-B mRNA expression (r=0.5, p=0.03) was also observed. These results suggest a co-regulation of NPR-B and CNP expression supporting the relevance of this receptor in human disease characterized by a marked inflammatory/immune component and suggesting the possibility of manipulating inflammation via pharmacological agents selective for this receptor.
authors.affiliation: CNR Institute of Clinical Physiology, Laboratory of Cardiovascular Biochemistry, Pisa, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Cabiati
authors.forename: L
authors.initials: L
authors.lastname: Sabatino
authors.forename: R
authors.initials: R
authors.lastname: Caruso
authors.forename: A
authors.initials: A
authors.lastname: Verde
authors.forename: C
authors.initials: C
authors.lastname: Caselli
authors.forename: T
authors.initials: T
authors.lastname: Prescimone
authors.forename: D
authors.initials: D
authors.lastname: Giannessi
authors.forename: S
authors.initials: S
authors.lastname: Del Ry
mesh_terms: D000328:Adult; D002908:Chronic Disease; D005260:Female; D020869:Gene Expression Profiling; D006333:Heart Failure / Q000097:blood / Q000235:genetics* / Q000503:physiopathology; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D020098:Natriuretic Peptide, C-Type / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism; D017461:Receptors, Atrial Natriuretic Factor / Q000235:genetics* / Q000378:metabolism; D012720:Severity of Illness Index; D000099242:Atrial Natriuretic Factor Receptor B
chemical_list: D020098:Natriuretic Peptide, C-Type; D012333:RNA, Messenger; D017461:Receptors, Atrial Natriuretic Factor; D000099242:Atrial Natriuretic Factor Receptor B
keywords: Blood; C-type natriuretic peptide; Heart failure; Human leukocytes; Natriuretic peptide receptor; Real-Time PCR; mRNA expression