title: Overexpression of Aldo-keto reductase family 1 B10 protein in ductal carcinoma in situ of the breast correlates with HER2 positivity.
abstract: BACKGROUND
  Ductal carcinoma in situ of the breast constitutes the early stage of breast cancer when cancer cells are confined by the intact myoepithelial cell layer. Transition from DCIS to invasive carcinoma is a process yet poorly understood.
  
  
  METHODS
  By liquid chromatography (LC) and mass spectrometry (MS/MS) methods, we analyzed this early event using paired samples of micro-dissected cells overlaid with focally disrupted myoepithelial layers and their adjacent counterparts within the intact duct from formalin-fixed paraffin-embedded blocks.
  
  
  RESULTS
  AKR1B10, a member of Aldo-keto reductase family, was shown to be abundantly located in the filtering cells among a catalog of proteins. Moreover, strong correlation between AKR1B10 and HER2 positivity was found in an independent cohort of DCIS samples.
  
  
  CONCLUSIONS
  AKR1B10 could become a potential diagnosis and therapeutic marker for early breast cancers with HER2 overexpression and poor prognosis.
authors.affiliation: College of Animal Science and Veterinary Medicine, Jilin University, Changchun, Jilin, China.
authors.forename: Zezhong
authors.initials: Z
authors.lastname: Li
authors.forename: Xin
authors.initials: X
authors.lastname: He
authors.forename: Shenyang
authors.initials: S
authors.lastname: Xing
authors.forename: Jinsong
authors.initials: J
authors.lastname: Ni
authors.forename: Weihong
authors.initials: W
authors.lastname: Zhang
authors.forename: Xiaofang
authors.initials: X
authors.lastname: Xu
authors.forename: Feng
authors.initials: F
authors.lastname: Gao
authors.forename: Jinhong
authors.initials: J
authors.lastname: Gai
authors.forename: Zhihui
authors.initials: Z
authors.lastname: Zhao
authors.forename: Jianhua
authors.initials: J
authors.lastname: Li
authors.forename: Pengtao
authors.initials: P
authors.lastname: Gong
authors.forename: Guocai
authors.initials: G
authors.lastname: Zhang
authors.forename: Xichen
authors.initials: X
authors.lastname: Zhang
mesh_terms: D000449:Aldehyde Reductase / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D000074408:Aldo-Keto Reductases; D014408:Biomarkers, Tumor / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D001943:Breast Neoplasms / Q000201:enzymology* / Q000473:pathology; D063646:Carcinogenesis; D002278:Carcinoma in Situ / Q000201:enzymology / Q000473:pathology; D018270:Carcinoma, Ductal, Breast / Q000201:enzymology* / Q000473:pathology; D002853:Chromatography, Liquid; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D013058:Mass Spectrometry; D042282:Microdissection; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism
chemical_list: D000449:Aldehyde Reductase; D000074408:Aldo-Keto Reductases; D014408:Biomarkers, Tumor; D018719:Erb-b2 Receptor Tyrosine Kinases; C120471:AKR1B10 protein, human; C508053:ERBB2 protein, human