title: Cloning and expression analysis of nonspecific cytotoxic cell receptor 1 (Ls-NCCRP1) from red snapper (Lutjanus sanguineus).
abstract: It is well known that nonspecific cytotoxic cells (NCCs) are kinds of natural killer cell mediated innate immune responses in teleosts. The nonspecific cytotoxic cell receptor protein 1 (NCCRP-1) is an important cell surface protein on NCC, which serves crucial functions in target cell recognition and cytotoxicity activation. In the present study, a nonspecific cytotoxic cell receptor protein NCCRP-1 (Ls-NCCRP1) was cloned from red snapper, Lutjanus sanguineus. The Ls-NCCRP1 cDNA is composed of 986bp with a 43bp of 5'-UTR, 702bp open reading frame (ORF) and 241bp 3'-UTR, encoding a polypeptide of 233 amino acids (GenBank accession no: ADK32635). Phylogenetic analysis revealed that Ls-NCCRP1 showed highest similarity to sea bream NCCRP-1. Quantitative real-time PCR (qRT-PCR) analysis showed that Ls-NCCRP1 had relatively high expression level in the head kidney, spleen and liver. After Vibrio alginolyticus infection, transcripts of Ls-NCCRP1 increased and reached its peak at 4h p.i. These results indicated that Ls-NCCRP1 may play an important role in innate immune response to bacteria.
authors.affiliation: College of Fishery, Guangdong Ocean University, Zhanjiang 524088, China; Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals, Zhanjiang 524088, China; Guangdong Key Laboratory of Control for Diseases of Aquatic Economic Animals, Zhanjiang 524088, China.
authors.forename: Jia
authors.initials: J
authors.lastname: Cai
authors.forename: Shina
authors.initials: S
authors.lastname: Wei
authors.forename: Bei
authors.initials: B
authors.lastname: Wang
authors.forename: Yucong
authors.initials: Y
authors.lastname: Huang
authors.forename: Jufen
authors.initials: J
authors.lastname: Tang
authors.forename: Yishan
authors.initials: Y
authors.lastname: Lu
authors.forename: Zaohe
authors.initials: Z
authors.lastname: Wu
authors.forename: Jichang
authors.initials: J
authors.lastname: Jian
mesh_terms: D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D029941:Fish Proteins / Q000235:genetics*; D007113:Immunity, Innate; D008969:Molecular Sequence Data; D010473:Perciformes / Q000235:genetics*; D010802:Phylogeny; D011946:Receptors, Antigen / Q000235:genetics*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D059467:Transcriptome; D044149:Vibrio alginolyticus
chemical_list: D018076:DNA, Complementary; D029941:Fish Proteins; D011946:Receptors, Antigen; C110644:nonspecific cytotoxic cell receptor protein 1
keywords: Innate immune; NCCRP-1; Red snapper; Vibrio alginolyticus