title: Genetic and molecular analyses of UniformMu transposon insertion lines.
abstract: The UniformMu transposon population is a large public resource for reverse genetics and functional genomics of maize. Users access the collection of UniformMu genetic stocks that are freely distributed by the Maize Cooperation Stock Center using online tools maintained at MaizeGDB.org. Genetic and molecular analyses of UniformMu stocks (UFMu insertion lines) typically require development of genotyping assays that use a gene-specific polymerase chain reaction (PCR) to follow segregation of transposon insertions in genes of interest. Here we describe methods for accessing the resource and recommended protocols for genotyping of transposon insertion alleles.
authors.affiliation: Horticultural Sciences Department, Plant Molecular and Cellular Biology Program, University of Florida, Gainesville, FL, USA.
authors.forename: Donald R
authors.initials: DR
authors.lastname: McCarty
authors.forename: Masaharu
authors.initials: M
authors.lastname: Suzuki
authors.forename: Charles
authors.initials: C
authors.lastname: Hunter
authors.forename: Joseph
authors.initials: J
authors.lastname: Collins
authors.forename: Wayne T
authors.initials: WT
authors.lastname: Avigne
authors.forename: Karen E
authors.initials: KE
authors.lastname: Koch
mesh_terms: D004251:DNA Transposable Elements / Q000235:genetics*; D060005:Genotyping Techniques / Q000379:methods*; D016254:Mutagenesis, Insertional / Q000235:genetics*; D054817:Pollination; D016133:Polymerase Chain Reaction; D003313:Zea mays / Q000235:genetics / Q000254:growth & development / Q000502:physiology
chemical_list: D004251:DNA Transposable Elements