title: HHV-6 is ubiquitously found using Western blot in tonsils and adenoid tissues of healthy people.
abstract: Few studies have examined the prevalence and cellular proclivity of latent human herpesvirus 6 (HHV-6) in healthy populations. Difficulties in detection of HHV-6 genome in different tissues using polymerase chain reaction (PCR) and immunohistochemistry (IHC) techniques have been reported by various researchers. We examined tonsils and adenoid tissues of 54 patients who had undergone tonsillectomy or adenoidectomy without any evidence of acute infection for the presence of latent HHV-6 infection. While we were investigating the prevalence of HHV-6, we tested the efficiency of PCR, IHC and Western Blot (WB) for detection of HHV-6 in tonsil tissues. We found that 100% of tonsil tissues were positive for HHV-6 with WB, 40% of tonsils were positive with PCR and no tonsil was positive with IHC. This result correlates well with most studies claiming HHV-6 is a ubiquitous organism in various populations and tissues. Western blot may be a good choice for detecting HHV-6 in tissues. Expression of the HHV-6 gp60/110 envelope protein disclosed by WB may indicate that HHV-6 does not have true latency. To our knowledge, this is the first report to use WB to test for HHV-6 in tissues.
authors.affiliation: Yuzuncu Yil University, School of Medicine, Department of Medical Biology, Turkey.
authors.forename: Mehmet
authors.initials: M
authors.lastname: Taspinar
authors.forename: Nilgun
authors.initials: N
authors.lastname: Cetin
authors.forename: Devran
authors.initials: D
authors.lastname: Gerceker
authors.forename: Djursun
authors.initials: D
authors.lastname: Karasartova
authors.forename: Buse
authors.initials: B
authors.lastname: Turegun
authors.forename: Sibel
authors.initials: S
authors.lastname: Ozturk
authors.forename: Fikret
authors.initials: F
authors.lastname: Sahin
mesh_terms: D000234:Adenoids / Q000821:virology*; D000293:Adolescent; D000328:Adult; D000956:Antigens, Viral / Q000276:immunology*; D015153:Blotting, Western / Q000379:methods*; D002648:Child; D002675:Child, Preschool; D004279:DNA, Viral / Q000235:genetics; D005260:Female; D006566:Herpesviridae Infections / Q000821:virology*; D015654:Herpesvirus 6, Human / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D009928:Organ Specificity; D014066:Palatine Tonsil / Q000821:virology*; D016133:Polymerase Chain Reaction; D015995:Prevalence; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA; D055815:Young Adult
chemical_list: D000956:Antigens, Viral; D004279:DNA, Viral