title: Proteome analysis of the anti-inflammatory response of flavonoids isolated from Korean Citrus aurantium L. in lipopolysaccharide-induced L6 rat skeletal muscle cells.
abstract: Skeletal muscle is an important organ in our body and a dynamic composite of proteins. Citrus aurantium L. has been widely used in oriental medicine in Eastern Asia for a long time. It contains over 100 bioactive compounds and flavonoids that regulate the inflammatory response and tumorigenesis, through various mechanisms. In the present study, we investigated changes in the protein pattern using two-dimensional electrophoresis (2-DE) and matrix assisted laser desorption ionization time of flight mass spectroscopy (MALDI-TOF/MS) to assess the anti-inflammatory effect of flavonoids isolated from Korean C. aurantium L. in lipopolysaccharide (LPS)-induced L6 cells. L6 skeletal muscle cells were pretreated with flavonoids for 1 h and stimulated with LPS for 24 h. Proteins from the L6 cells of the control, LPS treated and flavonoid treated groups were extracted and resolved by 2-DE using pH 4-7 IPG strips loaded with 150 μg of protein. Forty-one differentially expressed protein spots were identified (more than two-fold was considered significant, p < 0.05), and 18 were detected by MALDI-TOF/MS. These results suggest that proteomics can be used to identify changes in the expression of marker proteins and the anti-inflammatory effect of flavonoids isolated from Korean C. aurantium L.
authors.affiliation: Korea National Animal Research Resource Center, Korea National Animal Bio-Resource Bank, Research Institute of Life Science, Gazwa, Jinju, Republic of Korea.
authors.forename: Jin-A
authors.initials: JA
authors.lastname: Kim
authors.forename: Hyeon-Soo
authors.initials: HS
authors.lastname: Park
authors.forename: Kwang-Il
authors.initials: KI
authors.lastname: Park
authors.forename: Gyeong-Eun
authors.initials: GE
authors.lastname: Hong
authors.forename: Arulkumar
authors.initials: A
authors.lastname: Nagappan
authors.forename: Jue
authors.initials: J
authors.lastname: Zhang
authors.forename: Dae-Yong
authors.initials: DY
authors.lastname: Han
authors.forename: Sung-Chul
authors.initials: SC
authors.lastname: Shin
authors.forename: Chung-Gil
authors.initials: CG
authors.lastname: Won
authors.forename: Eun-Hee
authors.initials: EH
authors.lastname: Kim
authors.forename: Gon-Sup
authors.initials: GS
authors.lastname: Kim
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D002460:Cell Line; D002957:Citrus; D004586:Electrophoresis; D004731:Endotoxins / Q000633:toxicity; D005419:Flavonoids / Q000494:pharmacology*; D008070:Lipopolysaccharides / Q000633:toxicity; D018485:Muscle Fibers, Skeletal / Q000187:drug effects* / Q000378:metabolism; D020543:Proteome / Q000187:drug effects* / Q000378:metabolism; D051381:Rats; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D000893:Anti-Inflammatory Agents; D004731:Endotoxins; D005419:Flavonoids; D008070:Lipopolysaccharides; D020543:Proteome