title: Altered expression of adhesion molecules on peripheral blood leukocytes in feline infectious peritonitis.
abstract: Feline infectious peritonitis (FIP) is a fatal, coronavirus-induced systemic disease in domestic and wild felids. The pathology associated with FIP (multifocal granulomatous vasculitis) is considered to be elicited by exaggerated activation and subsequent extravasation of leukocytes. As changes in the expression of adhesion molecules on circulating leukocytes precede their margination and emigration, we reasoned that the expression of leukocyte adhesion molecules may be altered in FIP. In present study, the expression of principal adhesion molecules involved in leukocyte transmigration (CD15s, CD11a, CD11b, CD18, CD49d, and CD54) on peripheral blood leukocytes from cats with naturally occurring FIP (n=15) and controls (n=12) was quantified by flow cytometry using a formaldehyde-based rapid leukocyte preparation technique. T- and B-lymphocytes from FIP patients exhibit higher expression of both subunits (CD11a and CD18) composing the β2 integrin lymphocyte function-associated antigen (LFA)-1. In addition, the expression of the α4 subunit (CD49d) of the β1 integrin very late antigen (VLA)-4 was elevated on B-lymphocytes from FIP patients. The expression of CD11b and CD18, that combine to form the β2 integrin macrophage-1 antigen (Mac-1), was elevated on monocytes, whereas the density of CD49d was reduced on this population in FIP. Granulocytes of FIP cats displayed an increased expression of the α chain of Mac-1 (CD11b). These observations suggest that leukocytes from FIP patients show signs of systemic activation causing them to extravasate into surrounding tissues and ultimately contribute to pyogranuloma formation seen in FIP.
authors.affiliation: Department of Virology, Parasitology and Immunology, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, B-9820 Merelbeke, Belgium.
authors.forename: Dominique A J
authors.initials: DA
authors.lastname: Olyslaegers
authors.forename: Annelike
authors.initials: A
authors.lastname: Dedeurwaerder
authors.forename: Lowiese M B
authors.initials: LM
authors.lastname: Desmarets
authors.forename: Ben L
authors.initials: BL
authors.lastname: Vermeulen
authors.forename: Hannah L
authors.initials: HL
authors.lastname: Dewerchin
authors.forename: Hans J
authors.initials: HJ
authors.lastname: Nauwynck
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology / Q000378:metabolism; D002415:Cats; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D002478:Cells, Cultured; D016766:Feline Infectious Peritonitis / Q000276:immunology*; D005260:Female; D005434:Flow Cytometry; D007958:Leukocyte Count; D007962:Leukocytes / Q000166:cytology / Q000276:immunology / Q000378:metabolism*; D008297:Male
chemical_list: D015815:Cell Adhesion Molecules
keywords: Adhesion molecules; FIP; Flow cytometry; Peripheral blood leukocytes
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