title: Electron cryo-microscopy of TPPS4⋅2HCl tubes reveals a helical organisation explaining the origin of their chirality.
abstract: A widely studied achiral porphyrin, which is highly soluble in aqueous solutions (TPPS4), is shown to self-assemble into helical nanotubes. These were imaged by electron cryo-microscopy and a state-of-the-art image analysis allows building a map at ∼5 Å resolution, one of the highest obtained so far for molecular materials. The authors were able to trace the apparent symmetry breaking to existing nuclei in the "as received samples", while carefully purified samples show that both handnesses occur in equal amounts.
authors.affiliation: Division of Structural Studies, MRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge, CB1 0QH (UK). jms@mrc-lmb.cam.ac.uk.
authors.forename: Judith M
authors.initials: JM
authors.lastname: Short
authors.forename: John A
authors.initials: JA
authors.lastname: Berriman
authors.forename: Christian
authors.initials: C
authors.lastname: Kübel
authors.forename: Zoubir
authors.initials: Z
authors.lastname: El-Hachemi
authors.forename: Jean-Valère
authors.initials: JV
authors.lastname: Naubron
authors.forename: Teodor Silviu
authors.initials: TS
authors.lastname: Balaban
mesh_terms: D020285:Cryoelectron Microscopy / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D043942:Nanotubes / Q000737:chemistry; D011166:Porphyrins / Q000737:chemistry*; D012996:Solutions / Q000737:chemistry
chemical_list: D011166:Porphyrins; D012996:Solutions; C016136:tetraphenylporphine sulfonate
keywords: J‐aggregates/H‐aggregates; chirality; electron cryo‐microscopy; helical reconstruction; porphyrinoids; self‐assembly
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