title: Phylogeography and postglacial recolonization of Europe by Rhinolophus hipposideros: evidence from multiple genetic markers.
abstract: The demographic history of Rhinolophus hipposideros (lesser horseshoe bat) was reconstructed across its European, North African and Middle-Eastern distribution prior to, during and following the most recent glaciations by generating and analysing a multimarker data set. This data set consisted of an X-linked nuclear intron (Bgn; 543 bp), mitochondrial DNA (cytb-tRNA-control region; 1630 bp) and eight variable microsatellite loci for up to 373 individuals from 86 localities. Using this data set of diverse markers, it was possible to determine the species' demography at three temporal stages. Nuclear intron data revealed early colonization into Europe from the east, which pre-dates the Quaternary glaciations. The mtDNA data supported multiple glacial refugia across the Mediterranean, the largest of which were found in the Ibero-Maghreb region and an eastern location (Anatolia/Middle East)-that were used by R. hipposideros during the most recent glacial cycles. Finally, microsatellites provided the most recent information on these species' movements since the Last Glacial Maximum and suggested that lineages that had diverged into glacial refugia, such as in the Ibero-Maghreb region, have remained isolated. These findings should be used to inform future conservation management strategies for R. hipposideros and show the power of using a multimarker data set for phylogeographic studies.
authors.affiliation: School of Biology and Environmental Science, University College Dublin, Belfield, Dublin 4, Ireland.
authors.forename: Serena E
authors.initials: SE
authors.lastname: Dool
authors.forename: Sébastien J
authors.initials: SJ
authors.lastname: Puechmaille
authors.forename: Christian
authors.initials: C
authors.lastname: Dietz
authors.forename: Javier
authors.initials: J
authors.lastname: Juste
authors.forename: Carlos
authors.initials: C
authors.lastname: Ibáñez
authors.forename: Pavel
authors.initials: P
authors.lastname: Hulva
authors.forename: Stéphane G
authors.initials: SG
authors.lastname: Roué
authors.forename: Eric J
authors.initials: EJ
authors.lastname: Petit
authors.forename: Gareth
authors.initials: G
authors.lastname: Jones
authors.forename: Danilo
authors.initials: D
authors.lastname: Russo
authors.forename: Roberto
authors.initials: R
authors.lastname: Toffoli
authors.forename: Andrea
authors.initials: A
authors.lastname: Viglino
authors.forename: Adriano
authors.initials: A
authors.lastname: Martinoli
authors.forename: Stephen J
authors.initials: SJ
authors.lastname: Rossiter
authors.forename: Emma C
authors.initials: EC
authors.lastname: Teeling
mesh_terms: D000818:Animals; D001483:Base Sequence; D058578:Biglycan / Q000235:genetics; D002685:Chiroptera / Q000235:genetics*; D045303:Cytochromes b / Q000235:genetics; D004272:DNA, Mitochondrial / Q000235:genetics*; D005060:Europe; D019143:Evolution, Molecular; D005787:Gene Frequency; D005819:Genetic Markers / Q000235:genetics; D014644:Genetic Variation; D006239:Haplotypes / Q000235:genetics; D018895:Microsatellite Repeats / Q000235:genetics*; D058974:Phylogeography; D017422:Sequence Analysis, DNA
chemical_list: D058578:Biglycan; D004272:DNA, Mitochondrial; D005819:Genetic Markers; D045303:Cytochromes b
keywords: Anatolia; Chiroptera; Maghreb; Quaternary ice ages; Western Palearctic; glacial refugia