title: Binding of colicins A and El to purified TolA domains.
abstract: Colicins are divided into two groups according to the proteins required for their import into sensitive bacteria. The Tol and TonB pathways are involved in import of group A and group B colicins respectively. Because previous analyses have shown that colicin El and colicin A (two group A colicins) interact in vitro with the C-terminal domain of TolA (TolAlll) while colicin B (group B colicin)does not, attention was focused on these interactions with purified proteins.TolA has been described as a three-domain protein with an N-terminal inner-membrane anchor and a long periplasmic region formed by two domains(TolAII and TolAlll). TolAIII, TolAll and TolAII-Ill soluble domains with an N-terminal hexa-histidine extension were purified. The interactions of colicins with the purified TolA domains were analysed by overlay Western blotting,which indicated that both N-terminal domains of colicins A and E l interacted with TolAIII, while a gel shift procedure detected no interaction with colicin El.The binding kinetic values of the N-terminal domains of colicins A and E l to TolAlll were estimated by surface plasmon resonance and were shown to be similar.
authors.affiliation: Laboratoire d'Ingénierie et Dynamique des Systèmes Membranaires, UPR 9027, Institut de Biologie Structurale et Microbiologic, 13402 Marseille Cedex 20, France.
authors.forename: Rahmona
authors.initials: R
authors.lastname: Derouiche
authors.affiliation: Laboratoire d'Immunochimie des Peptides et Virus, UPR 9021, 67084 Strasbourg Cedex, France.
authors.forename: Gabrielle
authors.initials: G
authors.lastname: Zeder-Lutz
authors.affiliation: Laboratoire d'Ingénierie et Dynamique des Systèmes Membranaires, UPR 9027, Institut de Biologie Structurale et Microbiologic, 13402 Marseille Cedex 20, France.
authors.forename: Hélène
authors.initials: H
authors.lastname: Bénédetti
authors.affiliation: Laboratoire d'Ingénierie et Dynamique des Systèmes Membranaires, UPR 9027, Institut de Biologie Structurale et Microbiologic, 13402 Marseille Cedex 20, France.
authors.forename: Marthe
authors.initials: M
authors.lastname: Gavioli
authors.affiliation: Laboratoire d'Ingénierie et Dynamique des Systèmes Membranaires, UPR 9027, Institut de Biologie Structurale et Microbiologic, 13402 Marseille Cedex 20, France.
authors.forename: Alain
authors.initials: A
authors.lastname: Rigal
authors.affiliation: Laboratoire d'Ingénierie et Dynamique des Systèmes Membranaires, UPR 9027, Institut de Biologie Structurale et Microbiologic, 13402 Marseille Cedex 20, France.
authors.forename: Claude
authors.initials: C
authors.lastname: Lazdunski
authors.affiliation: Laboratoire d'Ingénierie et Dynamique des Systèmes Membranaires, UPR 9027, Institut de Biologie Structurale et Microbiologic, 13402 Marseille Cedex 20, France.
authors.forename: Roland
authors.initials: R
authors.lastname: LloubèAs
mesh_terms: D003087:Colicins / Q000737:chemistry / Q000378:metabolism*; D004926:Escherichia coli / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000378:metabolism*; D011485:Protein Binding
chemical_list: D003087:Colicins; D029968:Escherichia coli Proteins; C062093:tolA protein, E coli