title: [Simultaneous determination of six organic acids in Lonicerae japonicae flos and Lonicerae flos in different habitats by HPLC].
abstract: OBJECTIVE
  To establish an HPLC method for simultaneous determination of six organic acids in Lonicerae japonicae Flos and Lonicerae Flos in different habitats.
  
  
  METHODS
  The chromatographic separation was carried out on a Waters C18 column (4. 6 mm x 250 mm, 5 microm) with gradient elution of acetonitrile and 0.1% phosphoric acid at a flow rate of 1 mL/min. The detection wave-length was 326 nm.
  
  
  RESULTS
  The contents of neochlorogenic acid and cryptochlorogenic acid in Lonicerae japonicae Flos in different habitats were very low. The contents of chlorogenic acid in Lonicerae japonicae Flos in Pingyi, Fei County, Fengqiu and Jvlu were significantly higher than that in Shangluo, but the contents of other organic acids were similar. The contents of six kinds of organic acids in Lonicerae Flos in different origins were obviously higher than those in Lonicerae japonicae Flos.
  
  
  CONCLUSIONS
  A simple, accurate and credible method is developed and validated for quality control of six organic acids from Lonicerae japonicae Flos and Lonicerae Flos.
authors.affiliation: Shijiazhuang Yiling Pharmaceutical Co., Ltd., Shijiazhuang 050035, China. 479699662@qq.com
authors.forename: Min-Yan
authors.initials: MY
authors.lastname: Liu
authors.forename: Shu-Li
authors.initials: SL
authors.lastname: Gao
authors.forename: Li-Hua
authors.initials: LH
authors.lastname: Liu
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhang
authors.forename: Li-Jun
authors.initials: LJ
authors.lastname: Nian
authors.forename: Xiao-Yan
authors.initials: XY
authors.lastname: Liu
mesh_terms: D002726:Chlorogenic Acid / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004365:Drugs, Chinese Herbal / Q000032:analysis* / Q000737:chemistry; D035264:Flowers / Q000737:chemistry* / Q000254:growth & development; D031239:Lonicera / Q000737:chemistry* / Q000145:classification / Q000254:growth & development; D011786:Quality Control; D011801:Quinic Acid / Q000031:analogs & derivatives* / Q000032:analysis; D015203:Reproducibility of Results
chemical_list: C459206:1'-O-chlorogenoylneochlorogenic acid; D004365:Drugs, Chinese Herbal; D011801:Quinic Acid; D002726:Chlorogenic Acid