title: [Origin and the recombinant model of H7N9 virus prevailing in China].
abstract: OBJECTIVE
  To investigate the origin and the recombinant model of H7N9 virus prevailing in China by sequence analysis.
  
  
  METHODS
  The sequences of H7N9 virus were collected and analyzed with the software BLAST and MEGA 5.0. The phylogenetic trees were constructed after multiple sequences alignment. The homologous sequences of H7N9 segments were determined and the model was inferred according to the origin of H7N9 segments.
  
  
  RESULTS
  The most relevant sequences of HA, NA, NS and PB2 segments were located at one branch of the phylogenetic tree, while the closest relevant sequences of PB1, PA, NP and MP contained two H9N2 virus origins. According to the analysis of sequence origin, H7N9 viruses might be divided into 5 genotypes: namely A, B, A/Shanghai/1/2013-H7N9, A/Pigeon/Shanghai/S1069-H7N9 and A/Zhejiang/HZ1/2013-H7N9, and the genotype A consisted of A1 and A2 subtypes.
  
  
  CONCLUSIONS
  The prevailing H7N9 virus might be derived from 5 different viruses after 4 times of recombination, which resulted in the two major types of A and B. The subtypes of A1 and A2 were two different derivatives from one reassortant. The A/Pigeon/Shanghai/S1069-H7N9 strain might be the recombinant of type A H7N9 virus with a local H9N2 virus during the H7N9 epidemics. The A/Zhejiang/HZ1/2013-H7N9 strain could be the re-arrangement of subtype A2 with type B H7N9 virus.
authors.affiliation: Southern Medical University, Guangzhou, China. zhangsr@fimmu.com
authors.forename: Bao
authors.initials: B
authors.lastname: Zhang
authors.forename: Keyong
authors.initials: K
authors.lastname: Huang
authors.forename: Jinsong
authors.initials: J
authors.lastname: Guo
authors.forename: Xianbo
authors.initials: X
authors.lastname: Wu
authors.forename: Ling
authors.initials: L
authors.lastname: Li
authors.forename: Li
authors.initials: L
authors.lastname: Zhu
authors.forename: Chengsong
authors.initials: C
authors.lastname: Wan
authors.forename: Wei
authors.initials: W
authors.lastname: Zhao
mesh_terms: D002681:China; D016679:Genome, Viral; D005838:Genotype; D006801:Humans; D064766:Influenza A Virus, H7N9 Subtype / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D007251:Influenza, Human / Q000821:virology; D010802:Phylogeny; D016865:Reassortant Viruses / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D017423:Sequence Analysis, RNA; D014764:Viral Proteins / Q000235:genetics*
chemical_list: D014764:Viral Proteins