title: Vitronectin alters fibronectin organization at the cell-material interface.
abstract: Cells assemble fibronectin (FN) into fibrils in a process mediated by integrins. For this process to occur, it is known that the presence of other serum proteins is necessary. However, the individual effect of these proteins on FN fibrillogenesis has not been addressed so far. In this study, the effect of vitronectin (VN), an ECM adhesion protein, on material-driven FN fibrillogenesis and cell-mediated FN reorganization is investigated. Poly(ethyl acrylate), PEA, which has previously shown the ability to induce the organization of FN into well-developed physiological-like networks upon adsorption, was employed as a material substrate. FN adsorption, cell adhesion and cellular FN reorganization in the presence or absence of VN were studied. Both FN surface density, quantified via western blot, and its distribution on PEA surfaces, determined via atomic force microscopy, were altered when FN was adsorbed competitively with VN at certain compositions. Moreover, the presence of VN on the material surfaces enhanced cell-mediated FN reorganization and secretion, in comparison with the process which took place in the presence of serum proteins.
authors.affiliation: Center for Biomaterials and Tissue Engineering, Universitat Politècnica de València, Spain.
authors.forename: Cristina
authors.initials: C
authors.lastname: González-García
authors.affiliation: Division of Biomedical Engineering, School of Engineering, University of Glasgow, Glasgow G12 8LT, United Kingdom.
authors.forename: Marco
authors.initials: M
authors.lastname: Cantini
authors.affiliation: Center for Biomaterials and Tissue Engineering, Universitat Politècnica de València, Spain.
authors.forename: David
authors.initials: D
authors.lastname: Moratal
authors.affiliation: Institut de Bioenginyeria de Catalunya (IBEC), 08028 Barcelona, Spain; Institució Catalana de Recerca i Estudis Avançats (ICREA), 08010 Barcelona, Spain.
authors.forename: George
authors.initials: G
authors.lastname: Altankov
authors.affiliation: Division of Biomedical Engineering, School of Engineering, University of Glasgow, Glasgow G12 8LT, United Kingdom. Electronic address: Manuel.Salmeron-Sanchez@glasgow.ac.uk.
authors.forename: Manuel
authors.initials: M
authors.lastname: Salmerón-Sánchez
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry; D000327:Adsorption; D000818:Animals; D002448:Cell Adhesion; D005347:Fibroblasts / Q000166:cytology* / Q000378:metabolism*; D005353:Fibronectins / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D019012:Integrin beta1 / Q000378:metabolism; D051379:Mice; D041681:NIH 3T3 Cells; D011487:Protein Conformation; D019096:Vitronectin / Q000737:chemistry / Q000378:metabolism*
chemical_list: D000180:Acrylic Resins; D005353:Fibronectins; D019012:Integrin beta1; D019096:Vitronectin; C478961:poly(ethylacrylate)
keywords: Cell–material interface; Fibronectin; Fibronectin fibrillogenesis; Fibronectin reorganization; Vitronectin