title: Production and in vitro characterization of 3D porous scaffolds made of magnesium carbonate apatite (MCA)/anionic collagen using a biomimetic approach.
abstract: 3D porous scaffolds are relevant biomaterials to bone engineering as they can be used as templates to tissue reconstruction. The aim of the present study was to produce and characterize in vitro 3D magnesium-carbonate apatite/collagen (MCA/col) scaffolds. They were prepared by using biomimetic approach, followed by cross-linking with 0.25% glutaraldehyde solution (GA) and liofilization. Results obtained with Fourier-transform infrared spectroscopy (FT-IR) confirmed the type-B carbonate substitution, while by X-ray diffraction (XRD), a crystallite size of ~10nm was obtained. Optical and electron microscopy showed that the cylindrical samples exhibited an open-porous morphology, with apatite nanocrystals precipitated on collagen fibrils. The cross-linked 3D scaffolds showed integrity when immersed in culture medium up to 14 days. Also, the immersion of such samples into an acid buffer solution, to mimic the osteoclastic resorption environment, promotes the release of important ions for bone repair, such as calcium, phosphorus and magnesium. Bone cells (SaOs2) adhered, and proliferated on the 3D composite scaffolds, showing that synthesis and the cross-linking processes did not induce cytotoxicity.
authors.affiliation: Prog. Engenharia Metalúrgica e Materiais, COPPE/UFRJ, RJ, Brazil. msader@metalmat.ufrj.br
authors.forename: Marcia S
authors.initials: MS
authors.lastname: Sader
authors.forename: Virginia C A
authors.initials: VC
authors.lastname: Martins
authors.forename: Santiago
authors.initials: S
authors.lastname: Gomez
authors.forename: Racquel Z
authors.initials: RZ
authors.lastname: LeGeros
authors.forename: Gloria A
authors.initials: GA
authors.lastname: Soares
mesh_terms: D000818:Animals; D000838:Anions; D001031:Apatites / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D032701:Biomimetics / Q000379:methods*; D002417:Cattle; D002460:Cell Line; D003094:Collagen / Q000494:pharmacology*; D006801:Humans; D008855:Microscopy, Electron, Scanning; D016062:Porosity; D011208:Powders; D017550:Spectroscopy, Fourier Transform Infrared; D017154:Stromal Cells / Q000166:cytology / Q000187:drug effects / Q000648:ultrastructure; D054457:Tissue Scaffolds / Q000737:chemistry*; D014961:X-Ray Diffraction
chemical_list: D000838:Anions; D001031:Apatites; D011208:Powders; C586530:magnesium carbonate apatite; D003094:Collagen
keywords: 3D scaffold; Biomimetic approach; Collagen; Substituted apatite