title: Recruitment of the host plant heat shock protein 70 by Tomato yellow leaf curl virus coat protein is required for virus infection.
abstract: A functional capsid protein (CP) is essential for host plant infection and insect transmission of Tomato yellow leaf curl virus (TYLCV) and other monopartite begomoviruses. We have previously shown that TYLCV CP specifically interacts with the heat shock protein 70 (HSP70) of the virus insect vector, Bemisia tabaci. Here we demonstrate that during the development of tomato plant infection with TYLCV, a significant amount of HSP70 shifts from a soluble form into insoluble aggregates. CP and HSP70 co-localize in these aggregates, first in the cytoplasm, then in the nucleus of cells associated with the vascular system. CP-HSP70 interaction was demonstrated by co-immunopreciptation in cytoplasmic - but not in nuclear extracts from leaf and stem. Inhibition of HSP70 expression by quercetin caused a decrease in the amount of nuclear CP aggregates and a re-localization of a GFP-CP fusion protein from the nucleus to the cytoplasm. HSP70 inactivation resulted in a decrease of TYLCV DNA levels, demonstrating the role of HSP70 in TYLCV multiplication in planta. The current study reveals for the first time the involvement of plant HSP70 in TYLCV CP intracellular movement. As described earlier, nuclear aggregates contained TYLCV DNA-CP complexes and infectious virions. Showing that HSP70 localizes in these large nuclear aggregates infers that these structures operate as nuclear virus factories.
authors.affiliation: Institute of Plant Sciences and Genetics in Agriculture, Robert H. Smith Faculty of Agriculture, Food and Environment, Hebrew University of Jerusalem, Rehovot, Israel. gorovits@agri.huji.ac.il
authors.forename: Rena
authors.initials: R
authors.lastname: Gorovits
authors.forename: Adi
authors.initials: A
authors.lastname: Moshe
authors.forename: Murad
authors.initials: M
authors.lastname: Ghanim
authors.forename: Henryk
authors.initials: H
authors.lastname: Czosnek
mesh_terms: D000975:Antioxidants / Q000494:pharmacology; D053839:Begomovirus / Q000378:metabolism / Q000502:physiology*; D036022:Capsid Proteins / Q000378:metabolism*; D002467:Cell Nucleus / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism / Q000821:virology; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics* / Q000378:metabolism*; D018551:Solanum lycopersicum / Q000235:genetics / Q000378:metabolism / Q000821:virology*; D018515:Plant Leaves / Q000378:metabolism / Q000821:virology; D018547:Plant Stems / Q000378:metabolism / Q000821:virology; D011485:Protein Binding; D021381:Protein Transport / Q000187:drug effects; D011794:Quercetin / Q000494:pharmacology; D014760:Viral Fusion Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D000975:Antioxidants; D036022:Capsid Proteins; D018840:HSP70 Heat-Shock Proteins; D014760:Viral Fusion Proteins; D049452:Green Fluorescent Proteins; D011794:Quercetin
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