title: Crystallization and preliminary X-ray crystallographic analysis of cycloisomaltooligosaccharide glucanotransferase from Bacillus circulans T-3040.
abstract: Bacillus circulans T-3040 cycloisomaltooligosaccharide glucanotransferase (BcCITase) catalyses an intramolecular transglucosylation reaction and produces cycloisomaltooligosaccharides from dextran. BcCITase was overexpressed in Escherichia coli in two different forms and crystallized by the sitting-drop vapour-diffusion method. The crystal of BcCITase bearing an N-terminal His₆ tag diffracted to a resolution of 2.3 Å and belonged to space group P3₁21, containing a single molecule in the asymmetric unit. The crystal of BcCITase bearing a C-terminal His6 tag diffracted to a resolution of 1.9 Å and belonged to space group P2₁2₁2₁, containing two molecules in the asymmetric unit.
authors.affiliation: Biomolecular Research Unit, National Institute of Agrobiological Sciences, 2-1-2 Kannondai, Tsukuba 305-8602, Japan.
authors.forename: Nobuhiro
authors.initials: N
authors.lastname: Suzuki
authors.forename: Young Min
authors.initials: YM
authors.lastname: Kim
authors.forename: Mitsuru
authors.initials: M
authors.lastname: Momma
authors.forename: Zui
authors.initials: Z
authors.lastname: Fujimoto
authors.forename: Mikihiko
authors.initials: M
authors.lastname: Kobayashi
authors.forename: Atsuo
authors.initials: A
authors.lastname: Kimura
authors.forename: Kazumi
authors.initials: K
authors.lastname: Funane
mesh_terms: D001407:Bacillus / Q000201:enzymology*; D003460:Crystallization; D018360:Crystallography, X-Ray; D005964:Glucosyltransferases / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: D005964:Glucosyltransferases; C087517:cycloisomaltooligosaccharide glucanotransferase
keywords: Bacillus circulans; cycloisomaltooligosaccharide glucanotransferase; dextran; glycoside hydrolase family 66
references.citation: Acta Crystallogr D Biol Crystallogr. 2010 Jan;66(Pt 1):22-5
references.pmid: 20057045
references.citation: FEBS Lett. 1994 May 30;345(2-3):135-8
references.pmid: 7515357
references.citation: Biochem J. 1996 Jun 1;316 ( Pt 2):695-6
references.pmid: 8687420
references.citation: Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 Dec 1;67(Pt 12):1542-4
references.pmid: 22139161
references.citation: Methods Enzymol. 1997;276:307-26
references.pmid: 27754618
references.citation: Nucleic Acids Res. 2009 Jan;37(Database issue):D233-8
references.pmid: 18838391
references.citation: Biochem J. 1997 May 1;323 ( Pt 3):859-61
references.pmid: 9169623
references.citation: J Biol Chem. 2012 Jun 8;287(24):19916-26
references.pmid: 22337884
references.citation: FEBS J. 2012 Sep;279(17):3185-91
references.pmid: 22776355
references.citation: Biosci Biotechnol Biochem. 1995 Oct;59(10):1861-5
references.pmid: 8534976
references.citation: J Synchrotron Radiat. 2012 May;19(Pt 3):450-4
references.pmid: 22514184
references.citation: Appl Microbiol Biotechnol. 2011 Jul;91(2):329-39
references.pmid: 21479716
references.citation: J Biotechnol. 2007 Jun 15;130(2):188-92
references.pmid: 17433485
references.citation: Biochim Biophys Acta. 2012 Jul;1824(7):919-24
references.pmid: 22542750
references.citation: J Mol Biol. 1968 Apr 28;33(2):491-7
references.pmid: 5700707
references.citation: Biosci Biotechnol Biochem. 2006 Aug;70(8):1947-53
references.pmid: 16926507
references.citation: Biochim Biophys Acta. 2011 Mar;1814(3):428-34
references.pmid: 21193067
references.citation: Biosci Biotechnol Biochem. 2008 Dec;72(12):3277-80
references.pmid: 19060390
references.citation: J Biol Chem. 2012 Jun 8;287(24):19927-35
references.pmid: 22461618
references.citation: Biosci Biotechnol Biochem. 1993 Jan;57(7):1225-7
references.pmid: 27281012