title: The application of antimicrobial photodynamic therapy on S. aureus and E. coli using porphyrin photosensitizers bound to cyclodextrin.
abstract: Photodynamic therapy is usually used against malignant and non-malignant tumors. Nowadays, due to resistance of bacterial strains, we are looking for a new antimicrobial strategy to destroy bacteria with minimal invasive consequences. The worldwide increase in antibiotic resistance among different classes of gram-positive and gram-negative bacteria has led to the search for alternative anti-microbial therapies such as antimicrobial PDT (aPDT). Development antimicrobial technology combines a nontoxic compound, called photosensitizer, visible light of the appropriate wavelength, and the generation of reactive oxygen species. In this work, the photosensitizers TMPyP and ZnTPPS4 are investigated for photodynamic and antimicrobial photodynamic therapy. We tested these two porphyrins on two cell lines and two bacterial strains to compare effectiveness. In addition, we applied photosensitizers bound in the complex created with hp-β-cyclodextrin. The light-emitting diodes were used at the doses 0, 1, 5, 10 J/cm(2) for cells and 0, 150 J/cm(2) for bacteria. Tested concentrations for cells and microbes were from 0.5 to 50 μM and from 0.78 to 100 μM, respectively. From this work it can be concluded that TMPyP is a promising compound both in aPDT and in PDT, particularly in contrast to ZnTPPS4, which was efficient only in PDT. Furthermore, the eradication of gram-positive bacteria is possible only with higher concentrations of ZnTPPS4.
authors.affiliation: Department of Medical Biophysics, Faculty of Medicine and Dentistry and Institute of Molecular and Translational Medicine, Palacky University Olomouc, Czech Republic. Electronic address: a.hanakova@upol.cz.
authors.forename: Adela
authors.initials: A
authors.lastname: Hanakova
authors.affiliation: Department of Microbiology, Faculty of Medicine and Dentistry and Institute of Molecular and Translational Medicine, Palacky University Olomouc, Czech Republic.
authors.forename: Katerina
authors.initials: K
authors.lastname: Bogdanova
authors.affiliation: Department of Medical Biophysics, Faculty of Medicine and Dentistry and Institute of Molecular and Translational Medicine, Palacky University Olomouc, Czech Republic.
authors.forename: Katerina
authors.initials: K
authors.lastname: Tomankova
authors.affiliation: Department of Medical Biophysics, Faculty of Medicine and Dentistry and Institute of Molecular and Translational Medicine, Palacky University Olomouc, Czech Republic.
authors.forename: Klara
authors.initials: K
authors.lastname: Pizova
authors.affiliation: Department of Medical Biophysics, Faculty of Medicine and Dentistry and Institute of Molecular and Translational Medicine, Palacky University Olomouc, Czech Republic.
authors.forename: Jakub
authors.initials: J
authors.lastname: Malohlava
authors.affiliation: Department of Medical Biophysics, Faculty of Medicine and Dentistry and Institute of Molecular and Translational Medicine, Palacky University Olomouc, Czech Republic.
authors.forename: Svatopluk
authors.initials: S
authors.lastname: Binder
authors.affiliation: Department of Medical Biophysics, Faculty of Medicine and Dentistry and Institute of Molecular and Translational Medicine, Palacky University Olomouc, Czech Republic.
authors.forename: Robert
authors.initials: R
authors.lastname: Bajgar
authors.affiliation: Department of Medical Biophysics, Faculty of Medicine and Dentistry and Institute of Molecular and Translational Medicine, Palacky University Olomouc, Czech Republic.
authors.forename: Katerina
authors.initials: K
authors.lastname: Langova
authors.affiliation: Department of Microbiology, Faculty of Medicine and Dentistry and Institute of Molecular and Translational Medicine, Palacky University Olomouc, Czech Republic.
authors.forename: Milan
authors.initials: M
authors.lastname: Kolar
authors.affiliation: Department of Inorganic Chemistry, Faculty of Sciences, Charles University, Prague, Czech Republic.
authors.forename: Jiri
authors.initials: J
authors.lastname: Mosinger
authors.affiliation: Department of Medical Biophysics, Faculty of Medicine and Dentistry and Institute of Molecular and Translational Medicine, Palacky University Olomouc, Czech Republic.
authors.forename: Hana
authors.initials: H
authors.lastname: Kolarova
mesh_terms: D002460:Cell Line; D003505:Cyclodextrins / Q000737:chemistry / Q000494:pharmacology*; D004926:Escherichia coli / Q000187:drug effects* / Q000528:radiation effects*; D004927:Escherichia coli Infections / Q000188:drug therapy / Q000382:microbiology; D006801:Humans; D008027:Light; D010778:Photochemotherapy; D017319:Photosensitizing Agents / Q000737:chemistry / Q000494:pharmacology*; D011166:Porphyrins / Q000737:chemistry / Q000494:pharmacology*; D013203:Staphylococcal Infections / Q000188:drug therapy / Q000382:microbiology; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000528:radiation effects*
chemical_list: D003505:Cyclodextrins; D017319:Photosensitizing Agents; D011166:Porphyrins
keywords: Antimicrobial photodynamic therapy; Gram-negative bacteria; Gram-positive bacteria; Photodynamic therapy; Porphyrins