title: Molecular characterization of a short peptidoglycan recognition protein (PGRP-S) from Asian corn borer (Ostrinia furnacalis) and its role in triggering proPO activity.
abstract: Peptidoglycan recognition proteins (PGRPs) are non-specific immune molecules of insects, and vertebrates etc., but are not present in plants and nematodes. In the current experiment, a PGRP DNA sequence (2,910 bp containing four exons) was identified from genomic DNA library of Asian corn borer, Ostrinia furnacalis, and a full-length cDNA programming PGRP was cloned (designed as OfPGRP-S) with an open reading frame of 579 bp, having 192 amino acid. This inferred amino acid sequence showed maximum similarity to known lepidopteran PGRPs. Quantitative real-time PCR investigation disclosed the level of mRNA of OfPGRP-S to be constitutively expressed in the whole developmental stages and with higher expression in the mature larvae. Even more the OfPGRP-S was mainly expressed in immune capable organs i.e., fat body and midgut, and was strongly induced by injecting gram-positive bacteria i.e., Staphylococus aureus. Recombinant protein OfPGRP-S could bind to S. aureus and Bacillus thuringiensis which enhance proPO activation in the presence of these microbes. The results indicated that OfPGRP-S is an inducible protein acting as a receptor-type PGRP for enhancing the proPO activation on exposure to bacteria.
authors.affiliation: Engineering Research Center of Biological Control, Ministry of Education, South China Agricultural University, Guangzhou, 510642, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Sun
authors.forename: Xiao-Xia
authors.initials: XX
authors.lastname: Xu
authors.forename: Shoaib
authors.initials: S
authors.lastname: Freed
authors.forename: Wan-Jun
authors.initials: WJ
authors.lastname: Huang
authors.forename: Zhihua
authors.initials: Z
authors.lastname: Zheng
authors.forename: Shuang
authors.initials: S
authors.lastname: Wang
authors.forename: Shun-Xiang
authors.initials: SX
authors.lastname: Ren
authors.forename: Feng-Liang
authors.initials: FL
authors.lastname: Jin
mesh_terms: D000818:Animals; D001413:Bacillus thuringiensis / Q000378:metabolism; D002352:Carrier Proteins / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D004156:Catechol Oxidase / Q000378:metabolism*; D004792:Enzyme Precursors / Q000378:metabolism*; D020869:Gene Expression Profiling; D007915:Lepidoptera / Q000737:chemistry* / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D011485:Protein Binding; D060888:Real-Time Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D013211:Staphylococcus aureus / Q000378:metabolism
chemical_list: D002352:Carrier Proteins; D004792:Enzyme Precursors; D011994:Recombinant Proteins; C118897:peptidoglycan recognition protein; C038451:pro-phenoloxidase; D004156:Catechol Oxidase
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