title: Antifungal effect and pore-forming action of lactoferricin B like peptide derived from centipede Scolopendra subspinipes mutilans.
abstract: The centipede Scolopendra subspinipes mutilans has been a medically important arthropod species by using it as a traditional medicine for the treatment of various diseases. In this study, we derived a novel lactoferricin B like peptide (LBLP) from the whole bodies of adult centipedes, S. s. mutilans, and investigated the antifungal effect of LBLP. LBLP exerted an antifungal and fungicidal activity without hemolysis. To investigate the antifungal mechanism of LBLP, a membrane study with propidium iodide was first conducted against Candida albicans. The result showed that LBLP caused fungal membrane permeabilization. The assays of the three dimensional flow cytometric contour plot and membrane potential further showed cell shrinkage and membrane depolarization by the membrane damage. Finally, we confirmed the membrane-active mechanism of LBLP by synthesizing model membranes, calcein and FITC-dextran loaded large unilamellar vesicles. These results showed that the antifungal effect of LBLP on membrane was due to the formation of pores with radii between 0.74nm and 1.4nm. In conclusion, this study suggests that LBLP exerts a potent antifungal activity by pore formation in the membrane, eventually leading to fungal cell death.
authors.affiliation: School of Life Sciences and Biotechnology, College of Natural Sciences, Kyungpook National University, Daehak-ro 80, Buk-gu, Daegu 702-701, Republic of Korea.
authors.forename: Hyemin
authors.initials: H
authors.lastname: Choi
authors.forename: Jae-Sam
authors.initials: JS
authors.lastname: Hwang
authors.forename: Dong Gun
authors.initials: DG
authors.lastname: Lee
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000935:Antifungal Agents / Q000737:chemistry / Q000494:pharmacology*; D001181:Arthropods; D005452:Fluoresceins / Q000378:metabolism; D006461:Hemolysis / Q000187:drug effects; D007700:Kinetics; D007781:Lactoferrin / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D017386:Sequence Homology, Amino Acid
chemical_list: D000935:Antifungal Agents; D005452:Fluoresceins; D010455:Peptides; C082171:lactoferricin B; D007781:Lactoferrin; C007740:fluorexon
keywords: AMP; ATCC; Acetonitrile; American Type Culture Collection; Antifungal activity; Antimicrobial peptide; Antimicrobial peptides; Bis-(1,3-dibutylbarbituric acid trimethine oxonol; CFU; CH(3)CN; Centipede; Colony-forming unit; DiBAC(4)(3); FD; FITC; FITC-labeled dextran; Fluorescein isothiocyanate; KCTC; Korean Collection for Type Cultures; LBLP; LUV; Lactoferricin B like peptide; Large unilamellar vesicle; MIC; Minimum inhibitory concentration; PBS; PC; PE; PI; Phosphate buffered saline; Phosphatidylcholine; Phosphatidylethanolamine; Phosphatidylinositol; Pore-forming action; S. s. mutilans Assembled EST; SD; SmAE; Standard deviation