title: A novel monoclonal antibody specific for cocaine.
abstract: Detection systems for the illegal drug cocaine need to have a high sensitivity and specificity for cocaine and to be relatively easy to use. In the current study, a monoclonal antibody (MAb) with a high specificity for cocaine was produced. Enzyme-linked immunosorbent assay and fluorescence quenching immunoassay were used to screen the hybridomas. The MAb S27Y (IgG1) was shown to be sensitive and specific for cocaine and quenched fluorescence. Thus, S27Y has the potential to be used in screening assays for the rapid and sensitive detection of cocaine.
authors.affiliation: Bioscience Technology Development Office, R&D Division, Panasonic Co., Soraku-gun, Japan. nakayama.healther@jp.panasonic.com
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Nakayama
authors.forename: Noriko
authors.initials: N
authors.lastname: Kenjyou
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Shigetoh
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology* / Q000302:isolation & purification; D000917:Antibody Formation; D000918:Antibody Specificity; D003042:Cocaine / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005453:Fluorescence; D006825:Hybridomas / Q000276:immunology*; D018796:Immunoconjugates / Q000276:immunology*; D051379:Mice; D008805:Mice, Inbred A; D012680:Sensitivity and Specificity
chemical_list: D000911:Antibodies, Monoclonal; D018796:Immunoconjugates; D003042:Cocaine