title: Mitochondrial dysfunction contributes to the cytotoxicity induced by tentacle extract from the jellyfish Cyanea capillata in rat renal tubular epithelial NRK-52E cells.
abstract: Our previous studies have shown that tentacle extract (TE) from the jellyfish Cyanea capillata could induce a delayed jellyfish envenomation syndrome with severe multiple organ dysfunctions, among which renal injury with tubular necrosis seemed to be most serious. So, in this study, we aimed to explore the toxic effect of TE on rat renal tubular epithelial NRK-52E cells. Based on the previous findings that TE could cause oxidative damage in erythrocytes, the effects of TE on cell oxidative stress conditions, including ROS production and lipid peroxidation, and mitochondrial dysfunction associated with cell death were investigated in NRK-52E cells. The results showed that TE caused cell morphological change and decreased cell viability through induction of apoptosis and necrosis in NRK-52E cells. Meanwhile, ROS overproduction and mitochondrial membrane potential decrease were found before the cell death occurred. It was concluded that TE could induce cytotoxicity, especially apoptosis and necrosis, in NRK-52E cells, and mitochondrial dysfunction and ROS overproduction might play important roles in the process of cell injury and death.
authors.affiliation: Department of Marine Biotechnology, Faculty of Naval Medicine, Second Military Medical University, Shanghai 200433, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Wang
authors.forename: Qian
authors.initials: Q
authors.lastname: He
authors.forename: Liang
authors.initials: L
authors.lastname: Xiao
authors.forename: Qianqian
authors.initials: Q
authors.lastname: Wang
authors.forename: Bo
authors.initials: B
authors.lastname: Zhang
authors.forename: Beilei
authors.initials: B
authors.lastname: Wang
authors.forename: Guoyan
authors.initials: G
authors.lastname: Liu
authors.forename: Jiemin
authors.initials: J
authors.lastname: Zheng
authors.forename: Bentong
authors.initials: B
authors.lastname: Yu
authors.forename: Liming
authors.initials: L
authors.lastname: Zhang
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D003064:Cnidarian Venoms / Q000633:toxicity*; D004847:Epithelial Cells / Q000187:drug effects*; D007684:Kidney Tubules / Q000166:cytology; D015227:Lipid Peroxidation / Q000187:drug effects; D008928:Mitochondria / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D009336:Necrosis / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism; D037401:Scyphozoa / Q000737:chemistry*
chemical_list: D003064:Cnidarian Venoms; D017382:Reactive Oxygen Species
keywords: Cyanea capillata; Cytotoxicity; Jellyfish; Mitochondrial membrane potential; ROS; Renal tubular epithelial cells