title: [Effects of gastrin on rat intestinal epithelial 1,25(OH)2D3-membrane associated rapid response steroid binding protein].
abstract: OBJECTIVE
  To explore the effects of gastrin on the expression of 1,25(OH)2D3-membrane associated rapid response steroid (1,25D3-MARRS) binding protein in rat intestinal epithelium.
  
  
  METHODS
  SD rats received intraperitoneal injections of gastrin, omeprazole or physiological saline. The protein expression of 1,25D3-MARRS binding protein in SD rat intestinal was determined with Western blotting and immunohistochemistry, and its mRNA levels determined by RT-PCR. The serum calcium and phosphate levels in the rats were also detected.
  
  
  RESULTS
  Immunohistochemistry showed that 1,25D3-MARRS binding protein was expressed mainly in the nuclei, cytoplasm and membrane of the intestinal epithelial cells. Both the protein and mRNA expression levels of 1,25D3-MARRS binding protein were up-regulated after treatments with gastrin and omeprazole (P<0.05), but the serum calcium and phosphate concentrations showed no obvious increase.
  
  
  CONCLUSIONS
  1,25D3-MARRS binding protein, which is widely expressed with versatile functionalities, is regulated by gastrin and shows high potentials in the study of gastrointestinal diseases.
authors.affiliation: Department of Gastroenterology, Southern Medical University, Guangzhou, China. 19872186@163.com
authors.forename: Fenfen
authors.initials: F
authors.lastname: Liang
authors.forename: Cuiping
authors.initials: C
authors.lastname: Liu
authors.forename: Lixuan
authors.initials: L
authors.lastname: Li
authors.forename: Yu
authors.initials: Y
authors.lastname: Guo
authors.forename: Lan
authors.initials: L
authors.lastname: Bai
mesh_terms: D000818:Animals; D002117:Calcitriol / Q000378:metabolism; D004847:Epithelial Cells / Q000187:drug effects* / Q000378:metabolism*; D005755:Gastrins / Q000494:pharmacology*; D007422:Intestines / Q000166:cytology* / Q000187:drug effects; D008297:Male; D019704:Protein Disulfide-Isomerases / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D005755:Gastrins; C513196:PDIA3 protein, rat; D019704:Protein Disulfide-Isomerases; D002117:Calcitriol