title: [Expression of IL-27 in multiple myeloma and its cell lines].
abstract: OBJECTIVE
  To study the expression and significance of IL-27 in patients with multiple myeloma (MM) and in the supernatant of MM cell lines U266 and RPMI8226 cells culture medium.
  
  
  METHODS
  A total of 20 MM patients and 20 controls were enrolled in this study. The expressions of IL-27 and IL-6 in MM patient's blood plasma, and the expression of IL-27 in U266 and RPMI8226 culture supernatant were measured by enzyme-linked immunosorbent assay (ELISA). The mRNA expression of IL-27 in mononuclear cells of MM patients' peripheral blood was measured by real-time quantitative polymerase chain reaction (RT-PCR).
  
  
  RESULTS
  The levels of IL-27 in plasma of MM patients and normal controls were (61.82 ± 8.01) ng/L and (8.29 ± 4.41) ng/L (P<0.05), and those of IL-6 were (45.62 ± 1.24) ng/L and (2.27 ± 0.18) ng/L (P<0.05), respectively. The levels of IL-27 in U266 and RPMI8226 culture supernatant were (50.06 ± 5.72) ng/L and (335.47 ± 41.88) ng/L. RT-PCR revealed that the levels of IL-27 mRNA were up-regulated in MM patients compared to controls.
  
  
  CONCLUSIONS
  High expression of IL-27 is observed in MM patients and MM cell lines U266 and RPMI8226 cells. IL-27 may play a important role in pathogenesis of MM.
authors.affiliation: Department of Hematology, Qinghai Provincial People's Hospital, Medical College of Qinghai University, Xining 810001, China.
authors.forename: Tian
authors.initials: T
authors.lastname: Xia
authors.forename: Jian-ping
authors.initials: JP
authors.lastname: Li
authors.forename: Wen-qian
authors.initials: WQ
authors.lastname: Li
authors.forename: Li
authors.initials: L
authors.lastname: Wang
authors.forename: Xing
authors.initials: X
authors.lastname: Hao
authors.forename: Fang-fang
authors.initials: FF
authors.lastname: Sun
authors.forename: Xin
authors.initials: X
authors.lastname: Dai
authors.forename: Xiang-ren
authors.initials: XR
authors.lastname: A
authors.forename: Hai
authors.initials: H
authors.lastname: Peng
authors.forename: Yu-jun
authors.initials: YJ
authors.lastname: Song
authors.forename: Jian-ming
authors.initials: JM
authors.lastname: Feng
mesh_terms: D000328:Adult; D000368:Aged; D016022:Case-Control Studies; D045744:Cell Line, Tumor; D005260:Female; D006801:Humans; D015850:Interleukin-6 / Q000097:blood; D007378:Interleukins / Q000378:metabolism*; D008297:Male; D009101:Multiple Myeloma / Q000097:blood / Q000378:metabolism* / Q000473:pathology*
chemical_list: C508600:IL6 protein, human; D015850:Interleukin-6; D007378:Interleukins; C464565:MYDGF protein, human