title: N-acetylcysteine and deferrioxamine protects against acute renal failure induced by ischemia/reperfusion in rats.
abstract: OBJECTIVE
  Antioxidants are widely used in animal models to prevent renal injury after ischemia/reperfusion, but it is unknown if the benefits of antioxidants are additive. In this study, we aimed to investigate the protective effects of N-acetylcysteine plus deferoxamine in an animal model of kidney ischemia/reperfusion injury.
  
  
  METHODS
  Bilateral kidney ischemia was mastintained for 45 minutes. N-acetylcysteine, deferoxamine or both were administered into the aorta above the renal arteries immediately prior to induction of ischemia. Five rats from each group were sacrificed 1, 6 or 12 hours after reperfusion for the determination of blood creatinine, kidney oxidative damage parameters and myeloperoxidase activity.
  
  
  RESULTS
  The combination of N-acetylcysteine and deferoxamine, but not their isolated use, prevented the increase in creatinine after ischemia/reperfusion. This prevention was followed by a consistent decrease in myeloperoxidase activity and oxidative damage parameters both in the kidney cortex and medulla.
  
  
  CONCLUSIONS
  Treatment with N-acetylcysteine and deferoxamine was superior to the isolated use of either compound in an animal model of kidney ischemia/reperfusion.
authors.forename: Roberto Meister
authors.initials: RM
authors.lastname: Bernardi
authors.forename: Larissa
authors.initials: L
authors.lastname: Constantino
authors.forename: Roberta Albino
authors.initials: RA
authors.lastname: Machado
authors.forename: Francieli
authors.initials: F
authors.lastname: Vuolo
authors.forename: Patricia
authors.initials: P
authors.lastname: Budni
authors.forename: Cristiane
authors.initials: C
authors.lastname: Ritter
authors.forename: Felipe
authors.initials: F
authors.lastname: Dal-Pizzol