title: A single-stranded junction modulates nanosecond motional ordering of the substrate recognition duplex of a group I ribozyme.
abstract: Rigid spinning: Site-directed spin-labeling studies using a rigid nitroxide spin label (Ç) reveal that both length and sequence of a single-stranded junction (J1/2) modulate nanosecond motional ordering of the substrate-recognition duplex (P1) of the 120 kD group I ribozyme. The studies demonstrate an approach for experimental measurements of nanosecond dynamics in high-molecular-weight RNA complexes.
authors.affiliation: Department of Chemistry, University of Southern California, LJS-251, 840 Downey Way, Los Angeles, CA 90089-0744 (USA) http://pzqin.usc.edu/pzqhome.
authors.forename: Phuong
authors.initials: P
authors.lastname: Nguyen
authors.forename: Xuesong
authors.initials: X
authors.lastname: Shi
authors.forename: Snorri Th
authors.initials: ST
authors.lastname: Sigurdsson
authors.forename: Daniel
authors.initials: D
authors.lastname: Herschlag
authors.forename: Peter Z
authors.initials: PZ
authors.lastname: Qin
mesh_terms: D004578:Electron Spin Resonance Spectroscopy; D012313:RNA / Q000737:chemistry / Q000378:metabolism; D016337:RNA, Catalytic / Q000737:chemistry* / Q000378:metabolism; D013113:Spin Labels; D013379:Substrate Specificity; D013768:Tetrahymena / Q000378:metabolism; D013997:Time Factors
chemical_list: C116167:GIR1 ribozyme; D016337:RNA, Catalytic; D013113:Spin Labels; D012313:RNA
keywords: EPR spectroscopy; RNA; dynamics; motional ordering; spin labeling
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