title: Complete genomic sequence of Epstein-Barr virus in nasopharyngeal carcinoma cell line C666-1.
abstract: BACKGROUND
  Nasopharyngeal carcinoma is a distinct type of head and neck cancer which is consistently associated with Epstein-Barr virus (EBV). The C666-1 cell line is the only in vitro native EBV-infected NPC cell model commonly used for study of the viral-host interaction. Nevertheless, the complete EBV genome sequence in this in vitro EBV-infected NPC model has not been characterized.
  
  
  OBJECTIVE
  To determine the complete EBV genome sequence in C666-1 cells.
  
  
  METHODS
  The C666-1 genome was sequenced by 100-bases pair-end massive parallel sequencing. Bioinformatics analysis was performed to extract the EBV sequences and construct an EBV consensus sequence map. PCR amplification and Sanger DNA sequencing were used for sequence validation and gap filling. A phylogenetic analysis of EBV strain in C666-1 cells and other reported EBV strains was performed.
  
  
  RESULTS
  A 171,317 bp complete EBV genome of C666-1 was successfully constructed (GenBank accession number: KC617875). Phylogenetic analysis of EBV genome in C666-1 revealed that the C666-1 EBV strain is closely related to the reported strains in NPC primary tumors.
  
  
  CONCLUSIONS
  C666-1 contains a representative NPC-associated EBV genome and might serve as an important model for studying the roles or function of viral proteins in NPC tumorigenesis.
authors.affiliation: Department of Computer Science and Engineering, The Chinese University of Hong Kong, Hong Kong SAR, China.
authors.forename: Ken Kai-Yuen
authors.initials: KK
authors.lastname: Tso
authors.affiliation: Department of Computer Science and Engineering, The Chinese University of Hong Kong, Hong Kong SAR, China.
authors.forename: Kevin Yuk-Lap
authors.initials: KY
authors.lastname: Yip
authors.affiliation: Department of Anatomical and Cellular Pathology, State Key Laboratory in Oncology in South China, Prince of Wales Hospital, The Chinese University of Hong Kong, Hong Kong SAR, China.
authors.forename: Cathy Ka-Yan
authors.initials: CK
authors.lastname: Mak
authors.affiliation: Department of Anatomical and Cellular Pathology, State Key Laboratory in Oncology in South China, Prince of Wales Hospital, The Chinese University of Hong Kong, Hong Kong SAR, China.|Li Ka Shing Institute of Health Science, The Chinese University of Hong Kong, Hong Kong SAR, China.
authors.forename: Grace Tin-Yun
authors.initials: GT
authors.lastname: Chung
authors.affiliation: Department of Computer Science and Engineering, The Chinese University of Hong Kong, Hong Kong SAR, China.
authors.forename: Sau-Dan
authors.initials: SD
authors.lastname: Lee
authors.affiliation: Department of Surgery, University of Hong Kong, Hong Kong SAR, China.
authors.forename: Siu-Tim
authors.initials: ST
authors.lastname: Cheung
authors.affiliation: Department of Anatomical and Cellular Pathology, State Key Laboratory in Oncology in South China, Prince of Wales Hospital, The Chinese University of Hong Kong, Hong Kong SAR, China.|Li Ka Shing Institute of Health Science, The Chinese University of Hong Kong, Hong Kong SAR, China.
authors.forename: Ka-Fai
authors.initials: KF
authors.lastname: To
authors.affiliation: Department of Anatomical and Cellular Pathology, State Key Laboratory in Oncology in South China, Prince of Wales Hospital, The Chinese University of Hong Kong, Hong Kong SAR, China.|Li Ka Shing Institute of Health Science, The Chinese University of Hong Kong, Hong Kong SAR, China.
authors.forename: Kwok-Wai
authors.initials: KW
authors.lastname: Lo
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