title: Cenostigma macrophyllum Tul. on the healing of skin wounds in rats with diabetes mellitus.
abstract: OBJECTIVE
  To investigate the effect of Cenostigma macrophyllum Tul. in the tissue repair process of skin lesions in rats with induced Diabetes mellitus.
  
  
  METHODS
  Sixty-three male Wistar rats were distributed into three groups: control (C), diabetic (D), and diabetic treated with an oil-in-water emulsion of the plant (DPL) subdivided according to time of observation (seven, 14, and 28 days). Diabetes was induced by administration of by injection of streptozotocin (50 mg/kg, after a 12-h fast) into the penile vein and confirmed by glucose levels exceeding 240 mg/dL. Held surgical lesion (2.5 cm in diameter) on the back of the animals. The DPL received topical application of group of the oil-in-water emulsion plant (0.5 ml). To evaluate the levels of nitric oxide, was collected 5 ml of blood from the abdominal aorta, after his euthanasia. The samples of interest were sent for routine histological processing.
  
  
  RESULTS
  A reduction in the percentage of inflammatory cells and increased numbers of fibroblasts in the group DPL, seven days, compared to the other groups. At 14 days, the DPL group also showed a higher concentration of nitric oxide (p<0.01) than in groups C and D.
  
  
  CONCLUSIONS
  The oil-in-water emulsion of C. macrophyllum Tul accelerated wound healing in diabetic rats.
authors.affiliation: Postgraduate Program in Biomedical Engineering, Vale do Paraiba University, Sao Jose dos Campos, SP, Brazil. nayanamachado@oi.com.br
authors.forename: Nayana Pinheiro Machado de Freitas
authors.initials: NP
authors.lastname: Coelho
authors.forename: Veruska Cronemberger
authors.initials: VC
authors.lastname: Nogueira
authors.forename: Maria Angélica Gargione
authors.initials: MA
authors.lastname: Cardoso
authors.forename: Luciano da Silva
authors.initials: Lda S
authors.lastname: Lopes
authors.forename: Paulo Pedro do
authors.initials: PP
authors.lastname: Nascimento
authors.forename: Evair dos Santos
authors.initials: Edos S
authors.lastname: Rocha
authors.forename: Caio Leôncio Probo da
authors.initials: CL
authors.lastname: Silva
authors.forename: Emília Ângela Loschiavo
authors.initials: EÂ
authors.lastname: Arisawa
mesh_terms: D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000139:chemically induced / Q000503:physiopathology*; D004655:Emulsions; D007887:Fabaceae / Q000737:chemistry*; D005347:Fibroblasts; D007249:Inflammation / Q000188:drug therapy; D008297:Male; D009569:Nitric Oxide / Q000097:blood; D010936:Plant Extracts / Q000494:pharmacology*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D015203:Reproducibility of Results; D012867:Skin / Q000293:injuries* / Q000473:pathology; D013997:Time Factors; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D004655:Emulsions; D010936:Plant Extracts; D009569:Nitric Oxide