title: Generation and characterization of a tamoxifen-inducible Eomes(CreER) mouse line.
abstract: Transgenic mouse lines expressing inducible forms of Cre-recombinase in a tissue-specific manner are powerful genetic tools for studying aspects of development and various processes in the adult. The T-box transcription factor eomesodermin (Eomes) plays critical roles for maintenance and differentiation of different pools of stem and progenitor cells from early embryonic stages to adulthood. These include trophoblast stem cells, epiblast cells during the generation of the primary germ layers, neurogenic intermediate progenitor cells in embryonic and adult cortical neurogenesis, and maturing natural killer and T cells. Here, we report on the generation and analysis of an Eomes(CreER) -targeted allele by placing the tamoxifen-activatable Cre-recombinase (CreER) under the control of the Eomes genomic locus. We demonstrate that CreER expression recapitulates endogenous Eomes transcription within different progenitor cell populations. Tamoxifen administration specifically labels Eomes-expressing cells and their progeny as demonstrated by crossing Eomes(CreER) animals to different Cre-inducible reporter strains. In summary, this novel Eomes(CreER) allele can be used as elegant genetic tool that allows to follow the fate of Eomes-positive cells and to genetically manipulate them in a temporal specific manner.
authors.affiliation: University Medical Centre, Renal Department, Centre for Clinical Research, Freiburg, Germany.
authors.forename: Inga-Marie
authors.initials: IM
authors.lastname: Pimeisl
authors.forename: Yakup
authors.initials: Y
authors.lastname: Tanriver
authors.forename: Ray A
authors.initials: RA
authors.lastname: Daza
authors.forename: Franz
authors.initials: F
authors.lastname: Vauti
authors.forename: Robert F
authors.initials: RF
authors.lastname: Hevner
authors.forename: Hans-Henning
authors.initials: HH
authors.lastname: Arnold
authors.forename: Sebastian J
authors.initials: SJ
authors.lastname: Arnold
mesh_terms: D000483:Alleles; D000818:Animals; D005260:Female; D019426:Integrases / Q000235:genetics*; D008099:Liver / Q000196:embryology / Q000378:metabolism; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D023421:Models, Animal; D013154:Spleen / Q000196:embryology / Q000378:metabolism; D020825:T-Box Domain Proteins / Q000235:genetics*; D013629:Tamoxifen / Q000494:pharmacology*
chemical_list: C491198:Eomes protein, mouse; D020825:T-Box Domain Proteins; D013629:Tamoxifen; C045073:Cre recombinase; D019426:Integrases
keywords: Eomesodermin; Tbr2; lineage tracing; mouse; tamoxifen-activatable CreER
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