title: Newly developed TGF-β2 knock down transgenic mouse lines express TGF-β2 differently and its distribution in multiple tissues varies.
abstract: BACKGROUND
  Transforming growth factor-betas (TGF-βs), including beta2 (TGF-β2), constitute a superfamily of multifunctional cytokines with important implications in morphogenesis, cell differentiation and tissue remodeling. TGF-β2 is thought to play important roles in multiple developmental processes and neuron survival. However, before we carried out these investigations, a TGF-β2 gene down-regulated transgenic animal model was needed. In the present study, expressional silencing TGF-β2 was achieved by select predesigning interference short hairpin RNAs (shRNAs) targeting mouse TGF-β2 genes.
  
  
  RESULTS
  Four homozygous transgenic offspring were generated by genetic manipulation and the protein expressions of TGF-β2 were detected in different tissues of these mice. The transgenic mice were designated as Founder 66, Founder 16, Founder 53 and Founder 41. The rates of TGF-β2 down-expression in different transgenic mice were evaluated. The present study showed that different TGF-β2 expressions were detected in multiple tissues and protein levels of TGF-β2 decreased at different rates relative to that of wild type mice. The expressions of TGF-β2 proteins in transgenic mice (Founder 66) reduced most by 52%.
  
  
  CONCLUSIONS
  The present study generated transgenic mice with TGF-β2 down-regulated, which established mice model for systemic exploring the possible roles of TGF-β2 in vivo in different pathology conditions.
authors.affiliation: Institute of Neuroscience, Kunming Medical University, 1168 West Chunrong Road, Yuhua Avenue, Chenggong District, Kunming 650500, Yunnan, China.
authors.forename: Yan-Bin
authors.initials: YB
authors.lastname: Xiyang
authors.forename: Fang
authors.initials: F
authors.lastname: Wang
authors.forename: Bao-Jiang
authors.initials: BJ
authors.lastname: Qian
authors.forename: Ling
authors.initials: L
authors.lastname: You
authors.forename: Bing-Tuan
authors.initials: BT
authors.lastname: Lu
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: Xiong-Zhi
authors.initials: XZ
authors.lastname: Quan
authors.forename: Wen-Ping
authors.initials: WP
authors.lastname: Ge
authors.forename: Su
authors.initials: S
authors.lastname: Liu
authors.forename: Lian-Feng
authors.initials: LF
authors.lastname: Zhang
authors.forename: Ting-Hua
authors.initials: TH
authors.lastname: Wang
mesh_terms: D000818:Animals; D005786:Gene Expression Regulation; D005838:Genotype; D006720:Homozygote; D051379:Mice; D008822:Mice, Transgenic; D010641:Phenotype; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000378:metabolism; D053781:Transforming Growth Factor beta2 / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*
chemical_list: D034741:RNA, Small Interfering; D053781:Transforming Growth Factor beta2
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