title: [Functions of exogenous application of connective tissue growth factor in stimulating human dermal papilla cells and human hair follicle outer root sheath cells for reconstructive tissue-engineering hair follicles].
abstract: OBJECTIVE
  To explore the functions of connective tissue growth factor (CTGF) in the restoration of hair follicles with a mixture of human dermal papilla cells and human hair follicle outer root sheath cells in vitro in nude mice.
  
  
  METHODS
  Human hair follicle outer root sheath cells (hfORS) and human hair dermal papilla cells (hDP) were cultured in vitro and mixed in a fixed ratio (hfORS: hDP = 5:1). Flow cytometry was used to detect the content of CD200(+) cells in human hair follicle outer root sheath cells.And 8 nude mice were divided randomly into 2 groups according to a random number table and back wounds produced. Group A was transplanted with cell mixture plus 20 µg/L CTGF. Group B was transplanted with cell mixture alone. After 8 weeks of transplantation, the development of hair follicle formation was observed histologically.PCR was used to detect the expression of human specific DNA and mice DNA in transplants.
  
  
  RESULTS
  The portion of CD200(+) cells in cultured hfORS was 19.65%. At 8 weeks after implantation, hair follicle formation could be observed in Group A (268 ± 96) more than Group B (62 ± 20). The difference was statistically significant (P < 0.05). And PCR showed that there was human composition in transplant.
  
  
  CONCLUSIONS
  CTGF can induce the formation of hair follicle by promoting the interference between hDP and hfORS.
authors.affiliation: Department of Burns and Plastic Surgery, First Affiliated Hospital, General Hospital of PLA, Beijing, China.
authors.forename: Pei-pei
authors.initials: PP
authors.lastname: Zhang
authors.forename: Jia-ke
authors.initials: JK
authors.lastname: Chai
authors.forename: Ji-ping
authors.initials: JP
authors.lastname: Wang
authors.forename: Hong-jie
authors.initials: HJ
authors.lastname: Duan
authors.forename: Li
authors.initials: L
authors.lastname: Ma
authors.forename: Gui-ying
authors.initials: GY
authors.lastname: Zhu
mesh_terms: D000818:Animals; D017690:Cell Transplantation; D002478:Cells, Cultured; D055513:Connective Tissue Growth Factor / Q000494:pharmacology*; D018859:Hair Follicle / Q000166:cytology*; D006801:Humans; D008297:Male; D051379:Mice; D008819:Mice, Nude; D013234:Stem Cells / Q000166:cytology; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D055513:Connective Tissue Growth Factor