title: Backbone resonance assignments of the 42 kDa enzyme arginine kinase in the transition state analogue form.
abstract: Nearly complete backbone resonance assignments for the 357 residue, 42 kDa enzyme arginine kinase in a transition state analogue (TSA) complex are presented. The TSA is a quaternary complex of arginine kinase, MgADP, arginine, and nitrate. About 93% (320 of 344) of the non-proline backbone amides were assigned using an enzyme enriched with (2)H, (13)C, and (15)N in combination with three enzyme samples prepared with a single (15)N-labeled amino acid (K, L, and R). The amide assignments will provide the foundation for investigating the dynamics of arginine kinase when in a TSA complex.
authors.affiliation: Department of Biochemistry and Molecular Biology, Oregon Health and Science University, 3181 S.W. Sam Jackson Park Road, Portland, OR, 97239-3098, USA.
authors.forename: Omar
authors.initials: O
authors.lastname: Davulcu
authors.forename: Xiaogang
authors.initials: X
authors.lastname: Niu
authors.forename: Lei
authors.initials: L
authors.lastname: Brüschweiler-Li
authors.forename: Rafael
authors.initials: R
authors.lastname: Brüschweiler
authors.forename: Jack J
authors.initials: JJ
authors.lastname: Skalicky
authors.forename: Michael S
authors.initials: MS
authors.lastname: Chapman
mesh_terms: D001122:Arginine Kinase / Q000737:chemistry* / Q000378:metabolism; D055162:Biocatalysis; D004926:Escherichia coli / Q000201:enzymology; D019906:Nuclear Magnetic Resonance, Biomolecular
chemical_list: D001122:Arginine Kinase
references.citation: Annu Rev Physiol. 2001;63:289-325
references.pmid: 11181958
references.citation: J Biomol NMR. 2005 Jun;32(2):178
references.pmid: 16034675
references.citation: Structure. 2009 Oct 14;17(10):1356-67
references.pmid: 19836335
references.citation: Biochemistry. 2011 May 17;50(19):4011-8
references.pmid: 21425868
references.citation: Protein Sci. 2003 Jan;12(1):103-11
references.pmid: 12493833
references.citation: J Mol Biol. 2011 Jan 14;405(2):479-96
references.pmid: 21075117
references.citation: J Biomol NMR. 1998 Jul;12(1):1-23
references.pmid: 9729785
references.citation: Proc Natl Acad Sci U S A. 1998 Jul 21;95(15):8449-54
references.pmid: 9671698
references.citation: J Biol Chem. 2003 Jul 18;278(29):26952-7
references.pmid: 12732621
references.citation: J Biomol NMR. 1994 Mar;4(2):171-80
references.pmid: 8019132