title: Cloning of a sesquiterpene synthase from Lavandula x intermedia glandular trichomes.
abstract: The essential oil (EO) of Lavandula is dominated by monoterpenes, but can also contain small amounts of sesquiterpenes, depending on species and environmental conditions. For example, the sesquiterpene 9-epi-caryophyllene can make up to 8 % of the EO in a few species, including those commercially propagated for EO production. Here, we report the cloning and functional characterization of 9-epi-caryophyllene synthase (LiCPS) from the glandular trichomes of Lavandula x intermedia, cv. Grosso. The 1,617 bp open reading frame of LiCPS, which did not encode a transit peptide, was expressed in Escherichia coli and the recombinant protein purified by Ni-NTA agarose affinity chromatography. The ca. 60 kDa recombinant protein specifically converted farnesyl diphosphate to 9-epi-caryophyllene. LiCPS also produced a few monoterpenes when assayed with the monoterpene precursor geranyl diphosphate (GPP), but--unlike most monoterpene synthases--was not able to derive detectable amounts of any products from the cis isomer of GPP, neryl diphosphate. The LiCPS transcripts accumulated in developing L. x intermedia flowers and were highly enriched in glandular trichomes, but were not detected in leaves suggesting that the transcriptional expression of this gene is spatially and developmentally regulated.
authors.affiliation: University of British Columbia, Kelowna, BC, Canada.
authors.forename: Lukman S
authors.initials: LS
authors.lastname: Sarker
authors.forename: Zerihun A
authors.initials: ZA
authors.lastname: Demissie
authors.forename: Soheil S
authors.initials: SS
authors.lastname: Mahmoud
mesh_terms: D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D003433:Crosses, Genetic; D008401:Gas Chromatography-Mass Spectrometry; D018506:Gene Expression Regulation, Plant; D027523:Lavandula / Q000201:enzymology* / Q000235:genetics*; D008969:Molecular Sequence Data; D020411:Oligonucleotide Array Sequence Analysis; D009928:Organ Specificity / Q000235:genetics; D010802:Phylogeny; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid; D012717:Sesquiterpenes / Q000378:metabolism*; D064201:Trichomes / Q000201:enzymology* / Q000235:genetics*
chemical_list: D010940:Plant Proteins; D012333:RNA, Messenger; D011994:Recombinant Proteins; D012717:Sesquiterpenes
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