title: [The prevalence of dupA (duodenal ulcer-promoting gene) of Helicobacter pylori in children and adolescents--own observation].
abstract: The strains of Helicobacter pylori are described by many common features which determine their virulence. The genes which are connected with much higher virulence of some strains are vacA, cagA, oipA, dupA. Duodenal Ulcer Promoting Gene--dupA is the new virulence factor coexisting with a duodenum ulcer. There is a rationale that shows a protective character of dupA with reference to a stomach cancer. The dupA gene probably causes increasingly higher releasing of pro-infectious IL-8 via stomach cells and it influences the production of IL-12 and other cytokines. The aim of the study was to determine the frequency of dupA gene's appearance in the Polish children's group and in the Polish teenagers' group infected with H. pylori. The research was also aimed to determine the coexistence of dupA gene and duodenum ulcer disease or erosion infection of duodenum's mucous membrane.
  
  
  METHODS
  The endoscopic examination of the upper part of digestive duct was performed in 119 qualified patients with dyspeptic symptoms and with suspicion of stomach and duodenum's mucous membrane infection. The segments were taken for histopathological identification of H. pylori and for genetic indicating via PCR method. To confirm the presence of H. pylori in the extract the amplification of DNA fragment sized 860 pz was used. The presence of dupA gene was detected by PCR reaction with using the starters which include the fragment of jhp0917-jhp0918 sequence in the plastic H. pylori's genome area. To confirm the infection the urea breathing test was taken.
  
  
  RESULTS
  88 patients confirm the infection of H. pylori. The presence of dupA gene was found in 20 patients--a group A (22.7%), whereas in 68 patients dupA gene was not found--a group B (77.2%). Pathological changes in duodenum was found in 20 patients infected with H. pylori (22.7%), included 4 patients in the group A (20%) and 16 in the group B (23.5%). There was an infection (swelling, redness, congestion) in duodenum was found in the group A in all cases and there was an erosion presented in 3 patients. In the group B in 2 patients the duodenum ulcer disease was diagnosed. The infectious changes in duodenum were found in 7 patients but they were not infected with H. pylori; 1 patient was diagnosed with the duodenum ulcer disease.
  
  
  CONCLUSIONS
  The presence of dupA gene in the Polish children population infected with H. pylori is quite frequent but there is no clinical correlation with the duodenum ulcer disease.
authors.affiliation: Zakład Endoskopii i Badań Czynnościowych Przewodu Pokarmowego Wieku Rozwojowego, Uniwersytet Mikołaja Kopernika w Toruniu, Collegium Medicum im. Ludwika Rydygiera w Bydgoszczy. zendogasped@cm.umk.pl
authors.forename: Monika
authors.initials: M
authors.lastname: Parzecka
authors.forename: Anna
authors.initials: A
authors.lastname: Szaflarska-Popławska
authors.forename: Joanna
authors.initials: J
authors.lastname: Gasiorowska
authors.forename: Marta
authors.initials: M
authors.lastname: Gorzkiewicz
authors.forename: Tomasz
authors.initials: T
authors.lastname: Grzybowski
mesh_terms: D000293:Adolescent; D015984:Causality; D002648:Child; D002675:Child, Preschool; D015897:Comorbidity; D004381:Duodenal Ulcer / Q000453:epidemiology* / Q000235:genetics; D004386:Duodenum / Q000382:microbiology / Q000473:pathology; D005260:Female; D005753:Gastric Mucosa / Q000382:microbiology / Q000473:pathology; D016481:Helicobacter Infections / Q000453:epidemiology* / Q000235:genetics*; D016480:Helicobacter pylori / Q000235:genetics*; D006801:Humans; D008297:Male; D011044:Poland / Q000453:epidemiology; D015995:Prevalence; D037521:Virulence Factors / Q000302:isolation & purification*
chemical_list: C528428:DupA protein, Helicobacter pylori; D037521:Virulence Factors