title: Epigallocatechin-3-gallate exerts protective effects against heat stress through modulating stress-responsive transcription factors in poultry.
abstract: 1. The aim of the study was to describe the effect of Epigallocatechin-3-gallate (EGCG), a polyphenol derived from green tea, on activator protein-1 (AP-1) components (phospho-c-Jun and c-Fos), cyclooxygenase-2 (COX-2) and heat shock proteins (HSPs) in the liver of heat-stressed quails. 2. A total of 180 5-week-old female Japanese quails were reared either at 22°C for 24 h/d (thermoneutral, TN) or 34°C for 8 h/d (heat stress, HS) for 12 weeks. Birds in both environments were randomly given 1 of 3 diets: basal diet and basal diet with 200 or 400 mg of EGCG added per kilogram of diet. 3. The hepatic c-Jun, c-Fos, COX-2 and HSPs gene expression for quails reared under the HS environment was greater than those reared under the TN environment. Supplemental EGCG decreased hepatic expression of these proteins at a greater extent under HS than TN. 4. In conclusion, suppression of AP-1 COX-2 and HSPs may partly account for the inhibitory effect of EGCG in heat-stressed quail.
authors.affiliation: Department of Animal Nutrition , Faculty of Veterinary Medicine, Firat University, 23119 Elazig, Turkey.
authors.forename: C
authors.initials: C
authors.lastname: Orhan
authors.forename: M
authors.initials: M
authors.lastname: Tuzcu
authors.forename: H
authors.initials: H
authors.lastname: Gencoglu
authors.forename: N
authors.initials: N
authors.lastname: Sahin
authors.forename: A
authors.initials: A
authors.lastname: Hayirli
authors.forename: K
authors.initials: K
authors.lastname: Sahin
mesh_terms: D000821:Animal Feed / Q000032:analysis; D000818:Animals; D000906:Antibodies; D000975:Antioxidants / Q000008:administration & dosage / Q000378:metabolism*; D002392:Catechin / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000378:metabolism; D002645:Chickens; D003370:Coturnix / Q000254:growth & development / Q000502:physiology*; D051546:Cyclooxygenase 2 / Q000378:metabolism; D004032:Diet / Q000662:veterinary; D019587:Dietary Supplements / Q000032:analysis; D004305:Dose-Response Relationship, Drug; D005260:Female; D006360:Heat-Shock Proteins / Q000378:metabolism; D018869:Heat-Shock Response / Q000187:drug effects*; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D016760:Proto-Oncogene Proteins c-fos / Q000378:metabolism; D016755:Proto-Oncogene Proteins c-jun / Q000378:metabolism; D018808:Transcription Factor AP-1 / Q000378:metabolism
chemical_list: D000906:Antibodies; D000975:Antioxidants; D006360:Heat-Shock Proteins; D016760:Proto-Oncogene Proteins c-fos; D016755:Proto-Oncogene Proteins c-jun; D018808:Transcription Factor AP-1; D002392:Catechin; C045651:epigallocatechin gallate; D051546:Cyclooxygenase 2