title: Biomineralization on chemically synthesized collagen containing immobilized poly-γ-glutamic acid.
abstract: Chemically synthesized collagen with a triple helix structure similar to that of natural collagen is attractive as a safe biomaterial. Hybrids of chemically synthesized collagen and apatite are proposed for novel bone substitutes. However their apatite-forming ability in simulated body fluid is still quite low. We examined acceleration of apatite formation on collagen by immobilized poly-γ-glutamic acid (PGA), which has excellent apatite-forming ability. Apatite was formed within 3 days when collagen was treated with PGA solutions containing an appropriate amount of CaCl2. A mixture of apatite and calcite was formed at high CaCl2 concentration. The present results indicate the possibility of preparing hybrid materials, with tailored mechanical and biological properties, based on chemically synthesized collagen.
authors.affiliation: Graduate School of Life Science and Systems Engineering, Kyushu Institute of Technology, 2-4 Hibikino, Wakamatsu-ku, Kitakyushu 808-0196, Japan. tmiya@life.kyutech.ac.jp
authors.forename: Toshiki
authors.initials: T
authors.lastname: Miyazaki
authors.forename: Akimasa
authors.initials: A
authors.lastname: Kuramoto
authors.forename: Atsuhide
authors.initials: A
authors.lastname: Hirakawa
authors.forename: Yuki
authors.initials: Y
authors.lastname: Shirosaki
authors.forename: Chikara
authors.initials: C
authors.lastname: Ohtsuki
mesh_terms: D001031:Apatites / Q000138:chemical synthesis* / Q000737:chemistry; D040761:Biomimetic Materials / Q000138:chemical synthesis / Q000737:chemistry; D001826:Body Fluids / Q000737:chemistry; D002122:Calcium Chloride / Q000737:chemistry; D002234:Carbodiimides / Q000737:chemistry; D003094:Collagen / Q000138:chemical synthesis* / Q000737:chemistry; D003432:Cross-Linking Reagents / Q000737:chemistry; D006801:Humans; D006863:Hydrogen-Ion Concentration; D055767:Immobilized Proteins / Q000737:chemistry; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D011099:Polyglutamic Acid / Q000138:chemical synthesis* / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared; D013997:Time Factors; D014961:X-Ray Diffraction
chemical_list: C518491:1-ethyl-3-(3-(diethylamino)propyl)carbodiimide; D001031:Apatites; D002234:Carbodiimides; D003432:Cross-Linking Reagents; D055767:Immobilized Proteins; D011099:Polyglutamic Acid; D003094:Collagen; D002122:Calcium Chloride