title: Deletion of arcD in Streptococcus pneumoniae D39 impairs its capsule and attenuates virulence.
abstract: The arginine deiminase system (ADS) is associated with arginine catabolism and plays a role in virulence of several pathogenic bacteria. In Streptococcus pneumoniae, the ADS genes exist as a locus consisting of arcABCDT. A recent genome-wide mutagenesis approach revealed that both arcD and arcT are potentially essential in a chinchilla otitis media (OM) model. In the present study, we generated ΔarcD, ΔarcT, and ΔarcDT mutants by homologous recombination and evaluated their infectivity. Our results showed that only arcD, and not arcT, of an OM isolate is required during chinchilla middle ear infection. Additionally, D39 ΔarcD exhibited enhanced nasopharyngeal colonization and was attenuated in both mouse pneumonia and bacteremia models. In vitro, D39 ΔarcD displayed enhanced adherence to A549 epithelial cells and increased phagocytosis by J774A.1 macrophages compared to those with the parental strain. This mutant also exhibited an impaired capsule, as detected using electron microscopy, immunofluorescence, and a capsule assay. We demonstrated that the capsule defect in the D39 ΔarcD mutant may not be associated with a deficiency in arginine but rather is likely caused by a loss of interaction between the capsule and the transmembrane protein ArcD.
authors.affiliation: Center for Immunology and Microbial Disease, Albany Medical College, Albany, New York, USA.
authors.forename: Radha
authors.initials: R
authors.lastname: Gupta
authors.forename: Jun
authors.initials: J
authors.lastname: Yang
authors.forename: Yimin
authors.initials: Y
authors.lastname: Dong
authors.forename: Edwin
authors.initials: E
authors.lastname: Swiatlo
authors.forename: Jing-Ren
authors.initials: JR
authors.lastname: Zhang
authors.forename: Dennis W
authors.initials: DW
authors.lastname: Metzger
authors.forename: Guangchun
authors.initials: G
authors.lastname: Bai
mesh_terms: D026905:Amino Acid Transport Systems / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D017920:Antiporters / Q000235:genetics / Q000378:metabolism*; D016470:Bacteremia / Q000382:microbiology; D016667:Bacterial Capsules; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D002682:Chinchilla; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology*; D006801:Humans; D051379:Mice; D009154:Mutation; D010033:Otitis Media / Q000382:microbiology; D018410:Pneumonia, Bacterial / Q000382:microbiology; D013296:Streptococcus pneumoniae / Q000235:genetics / Q000378:metabolism* / Q000472:pathogenicity*; D014774:Virulence
chemical_list: D026905:Amino Acid Transport Systems; D017920:Antiporters; C063930:ArcD protein, Bacteria; D001426:Bacterial Proteins
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