title: ClpB1 overproduction in Synechocystis sp. strain PCC 6803 increases tolerance to rapid heat shock.
abstract: ClpB1 is a heat shock protein known to disaggregate large protein complexes. Constitutive, 16-fold ClpB1 overproduction in the cyanobacterium Synechocystis sp. strain PCC 6803 increased cell survival by 20-fold when cultures were heated quickly (1°C/s) to 50°C and delayed cell death by an average of 3 min during incubation at high temperatures (>46°C). Cooverexpression of ClpB1 and another heat shock protein, DnaK2, further increased cell survival. According to immunocytochemistry results, ClpB1 is dispersed throughout the cytoplasm but is concentrated in specific areas and is more prevalent near thylakoid membranes. However, ClpB1 overproduction does not lead to a change in the morphology, chlorophyll content, or photosystem ratio. Whereas electron microscopy demonstrated that apparent protein aggregation occurred after heat treatment in the control strain, protein aggregate size was maintained in the ClpB1 overexpresser. Constitutive ClpB1 overproduction allows an earlier response to heat shock and protects from rapid heating of cultures.
authors.affiliation: School of Life Sciences and Center for Bioenergy and Photosynthesis, Arizona State University, Tempe, Arizona, USA.
authors.forename: C Raul
authors.initials: CR
authors.lastname: Gonzalez-Esquer
authors.forename: Wim F J
authors.initials: WF
authors.lastname: Vermaas
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D003600:Cytosol / Q000737:chemistry; D015870:Gene Expression; D006360:Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism*; D006358:Hot Temperature; D007150:Immunohistochemistry; D050296:Microbial Viability / Q000528:radiation effects; D008854:Microscopy, Electron; D013312:Stress, Physiological; D046939:Synechocystis / Q000201:enzymology* / Q000528:radiation effects*
chemical_list: D001426:Bacterial Proteins; D006360:Heat-Shock Proteins
references.citation: mBio. 2012 Oct 02;3(5):
references.pmid: 23033472
references.citation: J Lipid Res. 2006 Apr;47(4):804-14
references.pmid: 16443931
references.citation: J Biotechnol. 2012 Nov 30;162(1):50-6
references.pmid: 22446641
references.citation: Annu Rev Genet. 1988;22:631-77
references.pmid: 2853609
references.citation: Plant Cell Physiol. 2001 Oct;42(10):1140-8
references.pmid: 11673630
references.citation: J Bacteriol. 1998 Oct;180(19):5173-82
references.pmid: 9748452
references.citation: J Electron Microsc (Tokyo). 1986;35(3):304-6
references.pmid: 2440973
references.citation: PLoS One. 2010 May 06;5(5):e10511
references.pmid: 20463904
references.citation: Plant Physiol. 2005 Jul;138(3):1409-21
references.pmid: 15965020
references.citation: Plant Mol Biol. 1987 Jul;8(4):317-26
references.pmid: 24301194
references.citation: J Exp Bot. 2006;57(7):1573-8
references.pmid: 16574748
references.citation: DNA Res. 1996 Jun 30;3(3):109-36
references.pmid: 8905231
references.citation: Cell. 1988 Nov 4;55(3):459-66
references.pmid: 2846183
references.citation: J Bacteriol. 2001 Dec;183(24):7392-6
references.pmid: 11717299
references.citation: Microbiology (Reading). 2004 Jan;150(Pt 1):247-259
references.pmid: 14702418
references.citation: Cell. 2003 Oct 17;115(2):229-40
references.pmid: 14567920
references.citation: J Bacteriol. 1996 Aug;178(16):4839-46
references.pmid: 8759846
references.citation: Appl Microbiol Biotechnol. 2004 Nov;65(6):635-48
references.pmid: 15300417
references.citation: Metab Eng. 2002 Jul;4(3):202-16
references.pmid: 12616690
references.citation: J Bacteriol. 2008 Oct;190(20):6758-68
references.pmid: 18689473
references.citation: Trends Biochem Sci. 1996 Aug;21(8):289-96
references.pmid: 8772382
references.citation: Mol Microbiol. 2003 Oct;50(2):585-95
references.pmid: 14617181
references.citation: J Mol Biol. 2009 Sep 25;392(3):589-601
references.pmid: 19596340
references.citation: J Biol Chem. 1957 May;226(1):497-509
references.pmid: 13428781
references.citation: Cell. 2006 Feb 10;124(3):535-47
references.pmid: 16469700
references.citation: Science. 2013 Mar 1;339(6123):1080-3
references.pmid: 23393091
references.citation: J Biol Chem. 2002 Nov 29;277(48):46310-8
references.pmid: 12297515
references.citation: FEBS Lett. 2005 Aug 15;579(20):4235-41
references.pmid: 16038902
references.citation: J Bacteriol. 2000 Dec;182(24):7092-6
references.pmid: 11092876
references.citation: Appl Environ Microbiol. 2000 Jan;66(1):64-72
references.pmid: 10618204
references.citation: J Bacteriol. 1991 Jul;173(14):4254-62
references.pmid: 2066329
references.citation: Plant J. 2006 Oct;48(2):249-60
references.pmid: 16995899
references.citation: FEMS Microbiol Lett. 2001 Nov 13;204(2):355-60
references.pmid: 11731148