title: Planifilum composti sp. nov., a thermophile isolated from compost.
abstract: Two thermophilic bacteria, designated strains P8(T) and P12, were isolated from compost in Korea. The isolates grew aerobically from 50 to 75 °C (optimum at 55 °C) and at pH 4.0-9.0 (optimum pH 6.5). Aerial mycelia were not observed. Single spores were produced along the substrate hypha. The predominant menaquinone was MK-7. Major fatty acids were iso-C17 : 0, iso-C15 : 0 and iso-C16 : 0. The cell wall contained meso-diaminopimelic acid and the polar lipids were phosphatidylethanolamine, an aminophospholipid and sphingoglycolipid. The DNA G+C contents were 55.9-56.5 mol%. Phylogenetic analyses based on 16S rRNA gene sequences indicated that strains P8(T) and P12 belonged to the genus Planifilum in the family Thermoactinomycetaceae with sequence similarities of 96.1-97.2 %. Levels of DNA-DNA relatedness between strain P8(T) and the type strains of recognized species of the genus Planifilum ranged from 28.9 to 38.2 %. On the basis of data from the present polyphasic study, strains P8(T) and P12 are considered to represent a novel species of the genus Planifilum, for which the name Planifilum composti sp. nov. is proposed. The type strain is P8(T) ( = KACC 16581(T) = NBRC 108858(T)).
authors.affiliation: Department of Microbial & Nano Materials, College of Science & Technology, Mokwon University, Doan-dong 800, Seo-gu, Daejeon 302-729, Republic of Korea.
authors.forename: Song-Ih
authors.initials: SI
authors.lastname: Han
authors.affiliation: Institute of Microbial Ecology and Resources, Mokwon University, Doan-dong 800, Seo-gu, Daejeon 302-729, Republic of Korea.
authors.forename: Jae-Chan
authors.initials: JC
authors.lastname: Lee
authors.affiliation: Department of Microbial & Nano Materials, College of Science & Technology, Mokwon University, Doan-dong 800, Seo-gu, Daejeon 302-729, Republic of Korea.
authors.forename: Hyo-Jin
authors.initials: HJ
authors.lastname: Lee
authors.affiliation: Institute of Microbial Ecology and Resources, Mokwon University, Doan-dong 800, Seo-gu, Daejeon 302-729, Republic of Korea.|Department of Microbial & Nano Materials, College of Science & Technology, Mokwon University, Doan-dong 800, Seo-gu, Daejeon 302-729, Republic of Korea.
authors.forename: Kyung-Sook
authors.initials: KS
authors.lastname: Whang
mesh_terms: D056449:Bacillales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D003960:Diaminopimelic Acid / Q000737:chemistry; D005227:Fatty Acids / Q000737:chemistry; D006358:Hot Temperature; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; D003960:Diaminopimelic Acid; C062629:menaquinone 7