title: Chemical introduction of the green fluorescence: imaging of cysteine cathepsins by an irreversibly locked GFP fluorophore.
abstract: An activity-based probe, containing an irreversibly locked GFP-like fluorophore, was synthesized and evaluated as an inhibitor of human cathepsins and, as exemplified with cathepsin K, it proved to be suitable for ex vivo imaging and quantification of cysteine cathepsins by SDS-PAGE.
authors.affiliation: Pharmaceutical Institute, Pharmaceutical Chemistry I, University of Bonn, An der Immenburg 4, 53121 Bonn, Germany. guetschow@uni-bonn.de.
authors.forename: Maxim
authors.initials: M
authors.lastname: Frizler
authors.forename: Ilia V
authors.initials: IV
authors.lastname: Yampolsky
authors.forename: Mikhail S
authors.initials: MS
authors.lastname: Baranov
authors.forename: Marit
authors.initials: M
authors.lastname: Stirnberg
authors.forename: Michael
authors.initials: M
authors.lastname: Gütschow
mesh_terms: D056657:Cathepsin K / Q000032:analysis; D002403:Cathepsins / Q000032:analysis*; D003545:Cysteine / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000032:analysis*; D049452:Green Fluorescent Proteins / Q000032:analysis*; D057809:HEK293 Cells; D006801:Humans
chemical_list: D005456:Fluorescent Dyes; D049452:Green Fluorescent Proteins; D002403:Cathepsins; D056657:Cathepsin K; D003545:Cysteine