title: [Proliferation characteristics of a PK-15 cell-adapted strain of porcine parvovirus].
abstract: To study the proliferation characteristics of PPV in differently infected way and the variance of concentrations in different cells. A strain of porcine parvovirus(PPV) was adapted to PK-15 cells, and a Real-time fluorescent quantitative PCR (FQ-PCR) assay was developed based on the specific region of the NS1 gene of PPV to quantify the PPV. The FQ-PCR was used to measure the viral concentration of virus-infected cells by simultaneous or step by step inoculation and plot one-step growth curves. The proliferation characteristics of PPV strain in different cells lines (HeLa, MDBK, PK-15 ,ST, F81, BHK-21 and Marc-145) was also compared. The results showed the PK-15 cell -adapted strain of PPV produced CPE after 12 passages, and maintained stable CPE at the following 10 messages. The one-step growth curve showed that the virus concentration of simultaneous inoculation was higher than that of the step-by-step inoculation, and the proliferation cycle of step-by-step inoculation was shorter. The proliferation ability of PPV strain in different cells showed that CPE appeared first inPK-15, followed by ST, HeLa and MDBK, and the virus concentration was highest in ST, followed byPK-15, MDBK and HeLa. NO proliferation was observed in F81, BHK-21 and Marc-145 cells. These findings lay a material foundation for the basic researches on PPV and the development of vaccine.
authors.affiliation: Animal Biotechnology Center, Ya'an 625014, China. wyf198674@163.com
authors.forename: Yun-Fei
authors.initials: YF
authors.lastname: Wu
authors.forename: Ling
authors.initials: L
authors.lastname: Zhu
authors.forename: Zhi-Wen
authors.initials: ZW
authors.lastname: Xu
authors.forename: Meng-Jin
authors.initials: MJ
authors.lastname: Fu
authors.forename: Lei
authors.initials: L
authors.lastname: Chen
authors.forename: Ai-Guo
authors.initials: AG
authors.lastname: Yang
authors.forename: Wan-Zhu
authors.initials: WZ
authors.lastname: Guo
mesh_terms: D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D003588:Cytopathogenic Effect, Viral; D004279:DNA, Viral / Q000235:genetics; D005260:Female; D000882:Haplorhini; D006801:Humans; D008297:Male; D010322:Parvoviridae Infections / Q000821:virology*; D028323:Parvovirus, Porcine / Q000235:genetics / Q000502:physiology*; D060888:Real-Time Polymerase Chain Reaction; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013552:Swine; D014764:Viral Proteins / Q000235:genetics*; D014779:Virus Replication
chemical_list: D004279:DNA, Viral; D014764:Viral Proteins