title: Magnetomotive optical coherence tomography for the assessment of atherosclerotic lesions using αvβ3 integrin-targeted microspheres.
abstract: OBJECTIVE
  We investigated the early-stage fatty streaks/plaques detection using magnetomotive optical coherence tomography (MM-OCT) in conjunction with αvβ3 integrin-targeted magnetic microspheres (MSs). The targeting of functionalized MSs was investigated by perfusing ex vivo aortas from an atherosclerotic rabbit model in a custom-designed flow chamber at physiologically relevant pulsatile flow rates and pressures.
  
  
  METHODS
  Aortas were extracted and placed in a flow chamber. Magnetic MS contrast agents were perfused through the aortas and MM-OCT, fluorescence confocal, and bright field microscopy were performed on the ex vivo aorta specimens for localizing the MSs.
  
  
  RESULTS
  The results showed a statistically significant and stronger MM-OCT signal (3.30 ± 1.73 dB) from the aorta segment perfused with targeted MSs, compared with the nontargeted MSs (1.18 ± 0.94 dB) and control (0.78 ± 0.41 dB) aortas. In addition, there was a good co-registration of MM-OCT signals with confocal microscopy.
  
  
  CONCLUSIONS
  Early-stage fatty streaks/plaques have been successfully detected using MM-OCT in conjunction with αvβ3 integrin-targeted magnetic MSs.
authors.affiliation: Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana-Champaign, 405 North Mathews Avenue, Urbana, IL, 61801, USA.
authors.forename: Jongsik
authors.initials: J
authors.lastname: Kim
authors.forename: Adeel
authors.initials: A
authors.lastname: Ahmad
authors.forename: Marina
authors.initials: M
authors.lastname: Marjanovic
authors.forename: Eric J
authors.initials: EJ
authors.lastname: Chaney
authors.forename: Joanne
authors.initials: J
authors.lastname: Li
authors.forename: Jonathan
authors.initials: J
authors.lastname: Rasio
authors.forename: Zita
authors.initials: Z
authors.lastname: Hubler
authors.forename: Darold
authors.initials: D
authors.lastname: Spillman
authors.forename: Kenneth S
authors.initials: KS
authors.lastname: Suslick
authors.forename: Stephen A
authors.initials: SA
authors.lastname: Boppart
mesh_terms: D000818:Animals; D001011:Aorta / Q000473:pathology; D050197:Atherosclerosis / Q000175:diagnosis*; D039302:Integrin alphaVbeta3 / Q000378:metabolism*; D060328:Magnetic Phenomena; D008297:Male; D008863:Microspheres; D011817:Rabbits; D041623:Tomography, Optical Coherence / Q000379:methods*
chemical_list: D039302:Integrin alphaVbeta3
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