title: In vitro evaluation of osteoconductivity and cellular response of zirconia and alumina based ceramics.
abstract: Developed ceria/yttria stabilized zirconia and ceria/yttria stabilized zirconia toughened alumina supported formation of apatite layer when immersed in simulated body fluid without any prior surface treatment. The formed mineral layer was confirmed as hydroxyapatite through X-ray diffraction patterns. The calcium/phosphate atomic ratio obtained from energy dispersive X-ray spectroscopy was found to be little less (Ca/P=1.5) than that of pure hydroxyapatite (Ca/P=1.7) which indicates the probability of mixed type calcium-phosphate compound formation. The achieved thickness of apatite layer was estimated through a surface profilometer and as high as ~17 μm thickness was found after 28 days of soaking. The biocompatibility of the developed materials was ensured through in vitro human osteoblast like cell (MG63) culture on ceramic discs. The morphology of attached cells was characterized through scanning electron microscopy and fluorescent microscopy which show multilayered interconnected cell growth within 8 days of culture period. Moreover, differentiation of MG63 cells was evaluated through MTT assay, total protein content and alkaline phosphatase activity.
authors.affiliation: Department of Metallurgical and Materials Engineering, Indian Institute of Technology, Kharagpur - 721 302, India. ajoy100@gmail.com
authors.forename: Ajoy Kumar
authors.initials: AK
authors.lastname: Pandey
authors.forename: Falguni
authors.initials: F
authors.lastname: Pati
authors.forename: Debika
authors.initials: D
authors.lastname: Mandal
authors.forename: Santanu
authors.initials: S
authors.lastname: Dhara
authors.forename: Koushik
authors.initials: K
authors.lastname: Biswas
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000537:Aluminum Oxide / Q000494:pharmacology*; D001861:Bone Regeneration / Q000187:drug effects*; D002118:Calcium / Q000032:analysis; D002448:Cell Adhesion / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D002516:Ceramics / Q000494:pharmacology*; D002563:Cerium / Q000494:pharmacology; D017886:Durapatite / Q000737:chemistry; D006244:Hardness; D006801:Humans; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects / Q000201:enzymology / Q000648:ultrastructure; D010316:Particle Size; D010758:Phosphorus / Q000032:analysis; D014961:X-Ray Diffraction; D015019:Yttrium / Q000494:pharmacology; D015040:Zirconium / Q000494:pharmacology*
chemical_list: D010758:Phosphorus; D002563:Cerium; D015019:Yttrium; C030583:ceric oxide; D017886:Durapatite; D015040:Zirconium; D000469:Alkaline Phosphatase; D000537:Aluminum Oxide; C028541:zirconium oxide; D002118:Calcium
keywords: Bio-ceramic; Bioactivity; Cell culture; In vitro biocompatibility; Osteoconduction