title: LipImage™ 815: novel dye-loaded lipid nanoparticles for long-term and sensitive in vivo near-infrared fluorescence imaging.
abstract: A new contrast agent, LipImage™ 815, has been designed and compared to previously described indocyanine green (ICG)-loaded lipid nanoparticles (ICG-lipidots®). Both contrast agents display similar size (50-nm diameter), zeta potential, high IC50 in cellular studies, near-infrared absorption and emission wavelengths in the "imaging window," long-term shelf colloidal and optical stabilities with high brightness (>106  L mol-1 cm-1) in ready-to-use storage conditions in aqueous buffer (4°C in dark), therefore being promising fluorescence contrast agents for in vivo imaging. However, while ICG-lipidots® display a relatively short plasma lifetime, LipImage™ 815 circulates in blood for longer times, allowing the efficient uptake of fluorescence signal in human prostate cancer cells implanted in mice. Prolonged tumor labeling is observed for more than 21 days.
authors.affiliation: CEA Grenoble, LETI-DTBS, MINATEC campus, F38054 Grenoble, France.
authors.forename: Aurélie
authors.initials: A
authors.lastname: Jacquart
authors.forename: Michelle
authors.initials: M
authors.lastname: Kéramidas
authors.forename: Julien
authors.initials: J
authors.lastname: Vollaire
authors.forename: Raphaël
authors.initials: R
authors.lastname: Boisgard
authors.forename: Géraldine
authors.initials: G
authors.lastname: Pottier
authors.forename: Emilie
authors.initials: E
authors.lastname: Rustique
authors.forename: Frédérique
authors.initials: F
authors.lastname: Mittler
authors.forename: Fabrice P
authors.initials: FP
authors.lastname: Navarro
authors.forename: Jérôme
authors.initials: J
authors.lastname: Boutet
authors.forename: Jean-Luc
authors.initials: JL
authors.lastname: Coll
authors.forename: Isabelle
authors.initials: I
authors.lastname: Texier
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D005260:Female; D005456:Fluorescent Dyes / Q000737:chemistry* / Q000493:pharmacokinetics / Q000633:toxicity; D006801:Humans; D008055:Lipids / Q000737:chemistry* / Q000493:pharmacokinetics; D008297:Male; D051379:Mice; D008819:Mice, Nude; D053758:Nanoparticles / Q000737:chemistry*; D061848:Optical Imaging / Q000379:methods*; D010316:Particle Size; D019265:Spectroscopy, Near-Infrared / Q000379:methods*; D014018:Tissue Distribution
chemical_list: D005456:Fluorescent Dyes; D008055:Lipids