title: Chromophobe renal cell carcinoma--chromosomal aberration variability and its relation to Paner grading system: an array CGH and FISH analysis of 37 cases.
abstract: Genetically, chromophobe renal cell carcinoma (ChRCC) is characterized by multiple chromosomal changes, especially losses. The most common losses include chromosomes 1, 2, 6, 10, 13, 17, and 21. The Fuhrman grading system lacks prognostic relevance for ChRCC, and recently, a new grading system for ChRCC was proposed by Paner. The objective of this study was to map the spectrum of chromosomal aberrations (extent and location) in a large cohort of ChRCCs and relate these findings to the Paner grading system (PGS). A large cohort of ChRCC was reviewed and graded according to the PGS. All the cases were reevaluated and separated into groups according to their PGS. The final study set was 37 patients. ChRCCs were divided into PG 1-3, sarcomatoid, and aggressive groups. "Aggressive ChRCCs" were designated cases with known metastatic activity, local recurrence, aggressive growth to the adjacent organs, or invasive growth into the renal sinus (with/without angioinvasion). Sarcomatoid tumors were divided into their epithelial and sarcomatoid component (further molecular genetic analyses were performed separately). Array comparative genome hybridization and/or fluorescence in situ hybridization analysis was applied to 42 samples from the 37 cases. Multiple losses, as well as gains, were detected in different chromosomes. Regardless of the PGS groups, the most frequently detected losses involved chromosomes 1 (27/37), 2 (26/37), 6 (23/37), 10 (26/37), 13 (19/37), and 17 (24/37). Loss of chromosome 21 was found in 12/37 cases. The most frequently detected gains were found on chromosomes 4 (22/37), 7 (24/37), 15 (20/37), 19 (22/37), and 20 (21/37). Cluster analysis showed that there is no relation between PGS and particular pattern of chromosomal changes (losses or gains) in ChRCCs. Conclusions are as follows: (1) ChRCCs showed a significantly broader spectrum of chromosomal aberrations than previously recognized. While previously published chromosomal losses were found in our cohort, gains of multiple chromosomes were also identified in a high percentage. The most frequently detected gains involved chromosomes 4, 7, 15, 19, and 20. (2) There is no relation between chromosomal numerical changes and Paner grading system.
authors.affiliation: Department of Pathology, East University, Riga, Latvia.
authors.forename: Maris
authors.initials: M
authors.lastname: Sperga
authors.forename: Petr
authors.initials: P
authors.lastname: Martinek
authors.forename: Tomas
authors.initials: T
authors.lastname: Vanecek
authors.forename: Petr
authors.initials: P
authors.lastname: Grossmann
authors.forename: Kevin
authors.initials: K
authors.lastname: Bauleth
authors.forename: Delia
authors.initials: D
authors.lastname: Perez-Montiel
authors.forename: Isabel
authors.initials: I
authors.lastname: Alvarado-Cabrero
authors.forename: Kristine
authors.initials: K
authors.lastname: Nevidovska
authors.forename: Vilnis
authors.initials: V
authors.lastname: Lietuvietis
authors.forename: Milan
authors.initials: M
authors.lastname: Hora
authors.forename: Michal
authors.initials: M
authors.lastname: Michal
authors.forename: Fredrik
authors.initials: F
authors.lastname: Petersson
authors.forename: Naoto
authors.initials: N
authors.lastname: Kuroda
authors.forename: Saul
authors.initials: S
authors.lastname: Suster
authors.forename: Jindrich
authors.initials: J
authors.lastname: Branzovsky
authors.forename: Ondrej
authors.initials: O
authors.lastname: Hes
mesh_terms: D002292:Carcinoma, Renal Cell / Q000235:genetics* / Q000473:pathology*; D002869:Chromosome Aberrations; D016000:Cluster Analysis; D055028:Comparative Genomic Hybridization; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007680:Kidney Neoplasms / Q000235:genetics* / Q000473:pathology*; D060787:Neoplasm Grading / Q000379:methods*; D020411:Oligonucleotide Array Sequence Analysis
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