title: Cell-repellant dextran coatings of porous titania using mussel adhesion chemistry.
abstract: The resistance of bioceramics against non-specific adsorption of serum proteins is critical for a wide range of biomedical applications. Some polysaccharides serve as natural protein-resistant molecules in extracellular matrices; however, the stable adhesion of polysaccharides to ceramic biomaterials in an aqueous solution is very challenging because chemical linkages at organic/inorganic interfaces are susceptible to hydrolytic degradation. Here, a catechol-grafted dextran, which strongly binds to titania (TiO2 ) in an aqueous milieu to effectively suppress cell adhesion through anti-fouling activity against non-specific protein adsorption, is introduced. Catechol is conjugated approximately to 6.7 mol% of glucose units of dextran via a carbamate ester linkage, corresponding to roughly three catechols per dextran chain having an average molecular weight of 6 kDa. Multivalent interactions of catechols with a titanium atom, enabled by the graft-type structure, provide a very stable coating of dextran on this inorganic surface. The adhesion of HeLa cells on the dextran-coated titania surface is reduced by 2.4-fold compared to that on a pristine titania surface. These results suggest that the graft-type incorporation of a small number of catechol moieties along a dextran backbone is an effective means of producing a stable anti-fouling interface on inorganic biomaterials in an aqueous environment.
authors.affiliation: Department of Material Science and Engineering, Korea Advanced Institute of Science and Technology, 291 Daehak-ro Yuseong-gu, Daejeon, 305-701, Republic of Korea.
authors.forename: Jae Yoon
authors.initials: JY
authors.lastname: Park
authors.forename: Jihyeon
authors.initials: J
authors.lastname: Yeom
authors.forename: Jee Seon
authors.initials: JS
authors.lastname: Kim
authors.forename: Mihyun
authors.initials: M
authors.lastname: Lee
authors.forename: Haeshin
authors.initials: H
authors.lastname: Lee
authors.forename: Yoon Sung
authors.initials: YS
authors.lastname: Nam
mesh_terms: D000818:Animals; D001798:Blood Proteins / Q000037:antagonists & inhibitors / Q000737:chemistry; D002240:Carbohydrate Sequence; D002396:Catechols / Q000737:chemistry*; D002448:Cell Adhesion / Q000187:drug effects; D020099:Coated Materials, Biocompatible / Q000138:chemical synthesis* / Q000494:pharmacology; D003911:Dextrans / Q000737:chemistry*; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D018625:Microscopy, Atomic Force; D008855:Microscopy, Electron, Scanning; D008969:Molecular Sequence Data; D016062:Porosity; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry*
chemical_list: D001798:Blood Proteins; D002396:Catechols; D020099:Coated Materials, Biocompatible; D003911:Dextrans; D049452:Green Fluorescent Proteins; C009495:titanium dioxide; D014025:Titanium; C034221:catechol
keywords: anti-fouling; catechol; cell adhesion; dextran; titanium dioxide