title: Psammaplysin F: a unique inhibitor of bacterial chromosomal partitioning.
abstract: Described is the antibiotic activity of a marine natural product. Psammaplysin F (1) inhibited the growth of four Gram-positive strains by >80% at 50μM, and the amine at position C-20 is responsible for the observed antibacterial activity. When tested against two strains of methicillin resistant Staphylococcus aureus (MRSA), the minimum inhibitory concentrations (MICs) for psammaplysin F (40-80μM) were similar to the structurally-related alkaloid psammaplysin H (2). Psammaplysin F (1) increased membrane permeability by two to four-fold compared to psammaplysin H (2) or control-treated bacteria, respectively. Unlike psammaplysin H (2), we show that psammaplysin F (1) inhibits equal partitioning of DNA into each daughter cell, suggesting that this natural product is a unique prokaryotic cell division inhibitor.
authors.affiliation: Department of Chemistry, University of New South Wales, Sydney, NSW 2052, Australia.
authors.forename: Deborah M
authors.initials: DM
authors.lastname: Ramsey
authors.forename: Md
authors.initials: M
authors.lastname: Amirul Islam
authors.forename: Lynne
authors.initials: L
authors.lastname: Turnbull
authors.forename: Rohan A
authors.initials: RA
authors.lastname: Davis
authors.forename: Cynthia B
authors.initials: CB
authors.lastname: Whitchurch
authors.forename: Shelli R
authors.initials: SR
authors.lastname: McAlpine
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D002876:Chromosomes, Bacterial / Q000378:metabolism*; D004269:DNA, Bacterial / Q000378:metabolism; D006801:Humans; D055624:Methicillin-Resistant Staphylococcus aureus / Q000166:cytology / Q000187:drug effects*; D011161:Porifera / Q000737:chemistry; D013141:Spiro Compounds / Q000737:chemistry / Q000494:pharmacology*; D013203:Staphylococcal Infections / Q000188:drug therapy / Q000382:microbiology; D014443:Tyrosine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D013141:Spiro Compounds; C107776:psammaplysin F; D014443:Tyrosine
keywords: Amine; Antibiotics; Cell division; DNA; Natural products