title: Transglutaminase 2 and phospholipase A₂ interactions in the inflammatory response in human Thp-1 monocytes.
abstract: Several experimental approaches have demonstrated that transglutaminase 2 (TG2) increased activity is involved in monocyte activation and inflammatory response. Preliminary results also demonstrate a TG-mediated post-translational modification of phospholipase A2 (PLA2), which catalyzes the release of arachidonic acid from its lipid storage sites. The control of PLA2-mediated production of eicosanoids has been found to be of great benefit for inflammatory disease treatment. However, the identification of the mechanisms of PLA2 activation is a very complex issue, because of the presence of multiple PLA2 forms. The aim of this study was to characterize the interactions between TG2 and sPLA2 in LPS-stimulated THP-1 cells, which were treated with TPA to induce early differentiated macrophage-type model. We demonstrated that increases in TG2 enzyme activity and protein expression may be considered an early event in monocyte/macrophage activation by LPS. Under these conditions, TG2 protein was co-immunoprecipitated with PLA2 by monoclonal antibody directed against the secretory form of the enzyme (sPLA2-V). Concomitantly, the PLA2 enzyme activity increased in TPA-treated cells exposed to LPS; these high levels of enzyme activity were significant reduced by R283, a site-specific inhibitor of TG2. Moreover, confocal laser scanning microscopy analysis of double-immunostained cytochemical specimens confirmed a co-localization of BAPA-labeled proteins and sPLA2-V in LPS-treated cells. These findings give evidence of a complex TG2/sPLA2-V, suggesting the possibility that sPLA2-V is a substrate for TG2. These results demonstrated that TG2 increases produced a sustained activation of PLA2 activity, suggesting a functional interaction between these enzymes in the regulation of inflammatory response.
authors.affiliation: Department of Biomedical Sciences and Morphological and Functional Imaging, University of Messina, AOU Policlinico "G. Martino", Via C. Valeria, 98125, Messina, Italy.
authors.forename: Monica
authors.initials: M
authors.lastname: Currò
authors.forename: Nadia
authors.initials: N
authors.lastname: Ferlazzo
authors.forename: Roberto
authors.initials: R
authors.lastname: Risitano
authors.forename: Salvatore
authors.initials: S
authors.lastname: Condello
authors.forename: Mercurio
authors.initials: M
authors.lastname: Vecchio
authors.forename: Daniela
authors.initials: D
authors.lastname: Caccamo
authors.forename: Riccardo
authors.initials: R
authors.lastname: Ientile
mesh_terms: D002478:Cells, Cultured; D019204:GTP-Binding Proteins / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D007249:Inflammation / Q000378:metabolism; D009000:Monocytes / Q000378:metabolism*; D054467:Phospholipases A2 / Q000737:chemistry / Q000378:metabolism*; D011485:Protein Binding; D000091345:Protein Glutamine gamma Glutamyltransferase 2; D011503:Transglutaminases / Q000737:chemistry / Q000378:metabolism*
chemical_list: D000091345:Protein Glutamine gamma Glutamyltransferase 2; D011503:Transglutaminases; D054467:Phospholipases A2; D019204:GTP-Binding Proteins