title: Immunohistochemical markers in the diagnosis of papillary thyroid carcinomas: The promising role of combined immunostaining using HBME-1 and CD56.
abstract: We aimed to evaluate the expression and diagnostic value of five immunohistochemical markers (HBME-1, Galectin-3, CK19, CD56 and p63) in a very large series of unequivocal papillary thyroid carcinoma (PTC) cases, including both the classic (CPTC) and the follicular variant (FVPTC). We performed an immunohistochemical analysis on a tissue micro-array of 204 PTCs (98 CPTCs, 90 FVPTCs, and 16 other variants). HBME-1 was the most sensitive marker, staining 95.9% of CPTCs and 81.1% of FVPTCs. CD56, a marker whose expression is reduced or absent in thyroid carcinoma, revealed a negative, "malignant" profile in 93.9% of CPTCs and 73.3% of FVPTCs. Galectin-3, CK19 and p63 were positive in 64.7%, 45.6% and 6.9% of PTCs, respectively. The immunopanel consisting of HBME-1, CD56 and/or CK19 reached the highest sensitivity (95.6%). The co-expression of 2 or more proteins was observed in 88.2% of PTCs, with HBME-1 and CD56 being the most frequent positive association (79.4%). We report a new panel of antibodies consisting of HBME-1, CK19 and CD56 that was found to be highly sensitive for both CPTC and FVPTC. This panel could be recommended as a supplement to the morphological criteria in the diagnosis of difficult FVPTC cases.
authors.affiliation: Department of Pathology, University of Medicine and Pharmacy, 38 Gheorghe Marinescu Street, Târgu-Mures 540000, Romania.
authors.forename: Adela
authors.initials: A
authors.lastname: Nechifor-Boila
authors.forename: Angela
authors.initials: A
authors.lastname: Borda
authors.forename: Geneviève
authors.initials: G
authors.lastname: Sassolas
authors.forename: Zakia
authors.initials: Z
authors.lastname: Hafdi-Nejjari
authors.forename: Françoise
authors.initials: F
authors.lastname: Borson-Chazot
authors.forename: Jean Christophe
authors.initials: JC
authors.lastname: Lifante
authors.forename: Nathalie
authors.initials: N
authors.lastname: Sturm
authors.forename: Marie Hélène
authors.initials: MH
authors.lastname: Lavérriere
authors.forename: Nicole
authors.initials: N
authors.lastname: Berger
authors.forename: Myriam
authors.initials: M
authors.lastname: Decaussin-Petrucci
mesh_terms: D000231:Adenocarcinoma, Papillary / Q000175:diagnosis* / Q000378:metabolism; D000328:Adult; D014408:Biomarkers, Tumor / Q000032:analysis* / Q000096:biosynthesis; D019002:CD56 Antigen / Q000032:analysis / Q000096:biosynthesis*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D053539:Keratin-19 / Q000032:analysis / Q000096:biosynthesis; D008297:Male; D012680:Sensitivity and Specificity; D013964:Thyroid Neoplasms / Q000175:diagnosis* / Q000378:metabolism; D046888:Tissue Array Analysis; D000951:Antigens, Neoplasm
chemical_list: D014408:Biomarkers, Tumor; D019002:CD56 Antigen; D053539:Keratin-19; C432845:HBME-1 antigen; D000951:Antigens, Neoplasm; C522918:NCAM1 protein, human
keywords: CD56 antigen; HBME-1 antigen; Immunohistochemistry; Papillary; Thyroid cancer