title: Mariniflexile soesokkakense sp. nov., a member of the family Flavobacteriaceae isolated from the junction between the North Pacific Ocean and a freshwater spring, and emended description of the genus Mariniflexile.
abstract: A Gram-negative, non-flagellated and rod-shaped bacterial strain able to move by gliding, designated RSSK-9(T), was isolated from the junction between the North Pacific Ocean and a freshwater spring at Jeju island, South Korea. Strain RSSK-9(T) was found to grow optimally at 30 °C, at pH 7.0-8.0 and in the presence of 2.0 % (w/v) NaCl. Phylogenetic trees based on 16S rRNA gene sequences revealed that strain RSSK-9(T) fell within the clade comprising Mariniflexile species. Strain RSSK-9(T) exhibits 16S rRNA gene sequence similarity values of 97.1-98.8 % to the type strains of Mariniflexile species and of less than 95.6 % sequence similarity to the type strains of the other recognized species. Strain RSSK-9(T) was found to contain MK-6 as the predominant menaquinone and iso-C15:0, iso-C15:1 G, iso-C17:0 3-OH and iso-C15:0 3-OH as the major fatty acids. The major polar lipids of strain RSSK-9(T) were identified as phosphatidylethanolamine and one unidentified lipid. The DNA G+C content of strain RSSK-9(T) was determined to be 34.6 mol% and its DNA-DNA relatedness values with the type strains of the four Mariniflexile species were in the range 11-28 %. The differential phenotypic properties, together with the phylogenetic and genetic distinctiveness, revealed that strain RSSK-9(T) is separate from other Mariniflexile species. On the basis of the data presented, strain RSSK-9(T) is considered to represent a novel species of the genus Mariniflexile, for which the name Mariniflexile soesokkakense sp. nov. is proposed. The type strain is RSSK-9(T) (= KCTC 32427(T) = CECT 8369(T)). An emended description of the genus Mariniflexile is given.
authors.affiliation: Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, South Korea.
authors.forename: Sooyeon
authors.initials: S
authors.lastname: Park
authors.forename: Yong-Taek
authors.initials: YT
authors.lastname: Jung
authors.forename: Jung-Hoon
authors.initials: JH
authors.lastname: Yoon
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D041965:Flavobacteriaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D005618:Fresh Water / Q000382:microbiology*; D006863:Hydrogen-Ion Concentration; D007723:Korea; D008124:Locomotion; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010140:Pacific Ocean; D010802:Phylogeny; D011809:Quinones / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride / Q000378:metabolism; D013696:Temperature
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D011809:Quinones; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride