title: Crystal structures of 26kDa Clonorchis sinensis glutathione S-transferase reveal zinc binding and putative metal binding.
abstract: The crystal structures of CsGST in two different space groups revealed that Asp26 and His79 coordinate a zinc ion. In one space group, His46 of an adjacent molecule participates in the coordination within 2.0Å. In the other space group, Asp26, His79 and a water molecule coordinate a zinc ion. The CsGST-D26H structure showed that four histidine residues - His26 and His79 from one molecule and the same residues from a symmetry-related neighboring molecule - coordinate a zinc ion. The coordinated zinc ions are located between two molecules and mediate molecular contacts within the crystal.
authors.affiliation: Division of Magnetic Resonance Research, Korea Basic Science Institute (KBSI), Ochang, Chungbuk 363-883, Republic of Korea.
authors.forename: Young-Hyun
authors.initials: YH
authors.lastname: Han
authors.forename: Sung-Jong
authors.initials: SJ
authors.lastname: Hong
authors.forename: Hae-Kap
authors.initials: HK
authors.lastname: Cheong
authors.forename: Yong Je
authors.initials: YJ
authors.lastname: Chung
mesh_terms: D000818:Animals; D001665:Binding Sites; D003004:Clonorchis sinensis / Q000201:enzymology*; D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000378:metabolism; D005982:Glutathione Transferase / Q000737:chemistry*; D006639:Histidine / Q000737:chemistry; D008670:Metals / Q000737:chemistry*; D008958:Models, Molecular; D009154:Mutation; D017434:Protein Structure, Tertiary; D015032:Zinc / Q000737:chemistry*
chemical_list: D008670:Metals; D006639:Histidine; D005982:Glutathione Transferase; D015032:Zinc
keywords: Crystal packing; Crystal structure; CsGST; CsGST–D26H mutant; Zinc coordination