title: Comparison of the salivary and the serum nitric oxide levels in chronic and aggressive periodontitis: a biochemical study.
abstract: OBJECTIVE
  Nitric oxide (NO) is a ubiquitous intercellular messenger molecule with important cardiovascular, neurological, and immune functions. In addition, it has been postulated that the pharmacological inhibition of NO or its actions may be therapeutically valuable in the disease management. The levels of nitric oxide may provide clues about the severity and the state of the underlying disease process. It could be an inflammatory biomarker that may enable clinicians to direct the environmentally based prevention or treatment programmes and to establish whether NO plays a role in the pathogenesis of periodontitis or not. Hence, the aim of the present study was to evaluate the salivary and the serum levels of NO in generalized chronic and aggressive periodontitis. The Study Design: Unstimulated whole saliva and serum samples were collected from a total of 60 subjects who were in the age group of 18-45 years, who participated in this study. They were divided into three equal groups with 20 subjects in each group; group A (healthy controls), group B (chronic periodontitis) and group C (aggressive periodontitis). The clinical parameters were assessed, based on the oral hygiene index simplified (OHI-S), the gingival index (GI), the probing pocket depth and the clinical attachment loss (CAL). A biochemical analysis was performed to evaluate and compare the salivary and the serum nitric oxide levels of the above groups. Statistical Analysis and Results: The statistical comparisons were done under the Griess Reaction. There were statistically significant salivary and serum levels of NO in the groups of periodontitis (group B and C) as compared to those in the healthy controls (group A). A significant positive correlation was found between the values of the salivary and the serum NO levels in chronic and aggressive periodontitis.
  
  
  CONCLUSIONS
  Nitric oxide is a potent modulator of the inflammatory disease processes and under pathological conditions, NO has damaging effects. As there is a paucity in the studies which have compared chronic and aggressive periodontitis, this study paved an interest for combining the serum and the salivary analysis in comparing the levels of nitric oxide in chronic and aggressive periodontitis.
authors.affiliation: Assistant Professor,Department of Periodontics, Sree Balaji Dental College , Chennai, India .
authors.forename: N Mani
authors.initials: NM
authors.lastname: Sundar
authors.forename: V
authors.initials: V
authors.lastname: Krishnan
authors.forename: S
authors.initials: S
authors.lastname: Krishnaraj
authors.forename: V T
authors.initials: VT
authors.lastname: Hemalatha
authors.forename: Md Nazish
authors.initials: MN
authors.lastname: Alam
keywords: Aggressive Periodontitis; Chronic Periodontitis; Nitric oxide (NO); Saliva; Serum
references.citation: J Clin Periodontol. 2001 Jun;28(6):565-8
references.pmid: 11350524
references.citation: J Clin Invest. 1997 Nov 1;100(9):2146-52
references.pmid: 9410890
references.citation: Clin Pharmacol Ther. 2001 Mar;69(3):89-95
references.pmid: 11240971
references.citation: J Periodontal Res. 2005 Apr;40(2):168-75
references.pmid: 15733152
references.citation: J Periodontal Res. 1998 Nov;33(8):517-8
references.pmid: 9879526
references.citation: Indian J Dent Res. 2006 Jul-Sep;17(3):117-20
references.pmid: 17176827
references.citation: Infect Immun. 2006 Dec;74(12):7010-3
references.pmid: 17015454
references.citation: J Oral Pathol Med. 2002 Nov;31(10):569-84
references.pmid: 12406302
references.citation: Clin Chim Acta. 2006 Apr;366(1-2):90-100
references.pmid: 16387291
references.citation: Periodontol 2000. 1997 Jun;14:9-11
references.pmid: 9567963
references.citation: Br J Rheumatol. 1998 Mar;37(3):246-57
references.pmid: 9566663
references.citation: Nitric Oxide. 2006 Mar;14(2):130-6
references.pmid: 16122953
references.citation: J Oral Sci. 2007 Dec;49(4):271-6
references.pmid: 18195509
references.citation: J Am Dent Assoc. 2006 Mar;137(3):313-21
references.pmid: 16570464
references.citation: J Periodontol. 2001 May;72(5):590-7
references.pmid: 11394393
references.citation: Ann Periodontol. 1999 Dec;4(1):1-6
references.pmid: 10863370
references.citation: Crit Rev Oral Biol Med. 1992;3(1-2):31-60
references.pmid: 1730070
references.citation: Arthritis Rheum. 1998 Jul;41(7):1141-51
references.pmid: 9663469
references.citation: Biochem Pharmacol. 1991 Oct 24;42(10):1849-57
references.pmid: 1720618
references.citation: J Indian Soc Periodontol. 2009 May;13(2):75-8
references.pmid: 20407654
references.citation: Crit Rev Oral Biol Med. 2002;13(2):197-212
references.pmid: 12097361