title: Characterization of the late endosomal ESCRT machinery in Trypanosoma brucei.
abstract: The multivesicular body (MVB) is a specialized Rab7+ late endosome (LE) containing multiple intralumenal vesicles that function in targeting ubiquitinylated cell surface proteins to the lysosome for degradation. African trypanosomes lack a morphologically well-defined MVB, but contain orthologs of the ESCRT (Endosomal Sorting Complex Required for Transport) machinery that mediates MVB formation. We investigate the role of TbVps23, an early ESCRT component, and TbVps4, the terminal ESCRT ATPase, in lysosomal trafficking in bloodstream form trypanosomes. Both localize to the TbRab7+ LE and RNAi silencing of each rapidly blocks growth. TbVps4 silencing results in approximately threefold accumulation of TbVps23 at the LE, consistent with blocking terminal ESCRT disassembly. Trafficking of endocytic and biosynthetic cargo, but not default lysosomal reporters, is also negatively affected. Others reported that TbVps23 mediates ubiquitin-dependent lysosomal degradation of invariant surface glycoproteins (ISG65) (Leung et al., Traffic 2008;9:1698-1716). In contrast, we find that TbVps23 ablation does not affect ISG65 turnover, while TbVps4 silencing markedly enhances lysosomal degradation. We propose several models to accommodate these results, including that the ESCRT machinery actually retrieves ISG65 from the LE to earlier endocytic compartments, and in its absence ISG65 traffics more efficiently to the lysosome. Overall, these results confirm that the ESCRT machinery is essential in Trypanosoma brucei and plays important and novel role(s) in LE function in trypanosomes.
authors.affiliation: Department of Microbiology and Immunology, School of Medicine and Biomedical Sciences, University at Buffalo (SUNY), 138 Farber Hall, 3435 Main Street, Buffalo, NY, 14214, USA; Current address: Monsanto Company, Mailstop LS2A, 800 N Lindbergh Blvd, Saint Louis, MO 63167, USA.
authors.forename: Jason S
authors.initials: JS
authors.lastname: Silverman
authors.forename: Katherine A
authors.initials: KA
authors.lastname: Muratore
authors.forename: James D
authors.initials: JD
authors.lastname: Bangs
mesh_terms: D056827:Endosomal Sorting Complexes Required for Transport / Q000378:metabolism*; D011992:Endosomes / Q000378:metabolism*; D008247:Lysosomes / Q000378:metabolism; D008562:Membrane Glycoproteins / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism; D057146:Multivesicular Bodies / Q000378:metabolism; D021381:Protein Transport; D022161:Transport Vesicles / Q000378:metabolism; D014346:Trypanosoma brucei brucei / Q000378:metabolism* / Q000502:physiology; D025801:Ubiquitin / Q000378:metabolism
chemical_list: D056827:Endosomal Sorting Complexes Required for Transport; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D025801:Ubiquitin
keywords: ESCRT; MVB; invariant surface glycoprotein; late endosome; lysosome; trypanosome
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