title: Molecular cloning and expression profile of a Halloween gene encoding Cyp307A1 from the seabuckthorn carpenterworm, Holcocerus hippophaecolus.
abstract: 20-Hydroxyecdyone, an active form of ecdysteroid, is the key hormone in insect growth and development. Halloween genes encode ecdysteroidogenic enzymes, including cytochrome P450 monooxygenase. CYP307A1 (spook) is accepted as an enzyme acting in the so-called 'black box' that includes a series of hypothetical and unproven reactions that finally result in the oxidation of 7-dehydrocholesterol to diketol. In this study, the Holcocerus hippophaecolus Hua (Lepidoptera: Cossidae) CYP307A1 (HhSpo) gene was identified and characterized. The obtained cDNA sequence was 2084 base pairs with an open reading frame of 537 animo acids, in which existed conserved motifs of CYP450 enzymes. The transcript profiles of HhSpo were analyzed in various tissues of final instar larvae. The highest expression was observed in the prothoracic gland, while expression level was low but significant in other tissues. These results suggest that the sequence character and expression profile of HhSpo were well conserved and provided the basic information for its functional analysis.
authors.affiliation: The Key Laboratory for Silviculture and Conservation, Ministry of Education, College of Forestry, Beijing Forestry University, Beijing 100083, P.R. China.
authors.forename: Jiao
authors.initials: J
authors.lastname: Zhou
authors.forename: Haolin
authors.initials: H
authors.lastname: Zhang
authors.forename: Juan
authors.initials: J
authors.lastname: Li
authors.forename: Xia
authors.initials: X
authors.lastname: Sheng
authors.forename: Shixiang
authors.initials: S
authors.lastname: Zong
authors.forename: Youqing
authors.initials: Y
authors.lastname: Luo
authors.forename: Kentaro
authors.initials: K
authors.lastname: Nagaoka
authors.forename: Qiang
authors.initials: Q
authors.lastname: Weng
authors.forename: Gen
authors.initials: G
authors.lastname: Watanabe
authors.forename: Kazuyoshi
authors.initials: K
authors.lastname: Taya
mesh_terms: D000818:Animals; D002417:Cattle; D003001:Cloning, Molecular; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics*; D015870:Gene Expression; D006801:Humans; D019476:Insect Proteins / Q000235:genetics*; D009036:Moths / Q000235:genetics*; D010802:Phylogeny; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D019476:Insect Proteins; D003577:Cytochrome P-450 Enzyme System
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