title: Dientamoeba fragilis DNA detection in Enterobius vermicularis eggs.
abstract: Dientamoeba fragilis is an intestinal protozoan suspected of causing gastrointestinal symptoms, and its mode of transmission is unknown, although first described almost a century ago. A hypothesis is that Enterobius vermicularis is a vector for D. fragilis, and recently, D. fragilis DNA was detected within surface-sterilized eggs of E. vermicularis. Using real-time PCR, we detected D. fragilis DNA in 18 (85%) of 21 samples of E. vermicularis eggs collected from patients harbouring D. fragilis in faeces. This finding supports the hypothesis that E. vermicularis may have an important role in the transmission of D. fragilis.
authors.affiliation: Clinical Microbiology Laboratory, Department of Laboratory Medicine, Division of Medical Services, County Hospital Ryhov, Jönköping, Sweden.
authors.forename: Jessica
authors.initials: J
authors.lastname: Ögren
authors.forename: Olaf
authors.initials: O
authors.lastname: Dienus
authors.forename: Sture
authors.initials: S
authors.lastname: Löfgren
authors.forename: Peter
authors.initials: P
authors.lastname: Iveroth
authors.forename: Andreas
authors.initials: A
authors.lastname: Matussek
mesh_terms: D000818:Animals; D016054:DNA, Protozoan / Q000235:genetics / Q000302:isolation & purification*; D004029:Dientamoeba / Q000235:genetics / Q000302:isolation & purification*; D004030:Dientamoebiasis / Q000150:complications / Q000469:parasitology; D017229:Enterobiasis / Q000150:complications / Q000469:parasitology; D004757:Enterobius / Q000469:parasitology*; D060888:Real-Time Polymerase Chain Reaction; D015053:Zygote / Q000469:parasitology*
chemical_list: D016054:DNA, Protozoan
keywords: DNA; Dientamoeba fragilis; Entrobius vermicularis; transmission
references.citation: Parasitology. 2011 Apr;138(5):557-72
references.pmid: 21349214
references.citation: Parasitol Int. 2008 Mar;57(1):72-5
references.pmid: 17921047
references.citation: Gut Microbes. 2011 Jan-Feb;2(1):3-12
references.pmid: 21637013
references.citation: Mol Cell Probes. 2007 Oct-Dec;21(5-6):400-4
references.pmid: 17587544
references.citation: Clin Microbiol Infect. 2003 Feb;9(2):110-3
references.pmid: 12588330
references.citation: Clin Microbiol Rev. 2004 Jul;17(3):553-70, table of contents
references.pmid: 15258093
references.citation: Exp Parasitol. 2013 Jan;133(1):57-61
references.pmid: 23116599
references.citation: Am J Trop Med Hyg. 2010 Apr;82(4):614-9
references.pmid: 20348509
references.citation: Am J Trop Med Hyg. 1956 Mar;5(2):258-65
references.pmid: 13302621