title: Spirosoma endophyticum sp. nov., isolated from Zn- and Cd-accumulating Salix caprea.
abstract: A Gram-reaction-negative, yellow-pigmented strain, designated EX36(T), was characterized using a polyphasic approach comprising phylogenetic, morphological and genotypic analyses. The endophytic strain was isolated from Zn/Cd-accumulating Salix caprea in Arnoldstein, Austria. Analysis of the 16S rRNA gene demonstrated that the novel strain is most closely related to members of the genus Spirosoma (95 % sequence similarity with Spirosoma linguale). The genomic DNA G+C content was 47.2 mol%. The predominant quinone was and the major cellular fatty acids were summed feature 3 (iso-C15 : 0 2-OH and/or C16 : 1ω7c), C16 : 1ω5c, iso-C17 : 0 3-OH and iso-C15 : 0. On the basis of its phenotypic and genotypic properties, strain EX36(T) should be classified as a novel species of the genus Spirosoma, for which the name Spirosoma endophyticum sp. nov. is proposed. The type strain is EX36(T) ( = DSM 26130(T) = LMG 27272(T)).
authors.affiliation: AIT Austrian Institute of Technology GmbH, Bioresources Unit, Tulln, Austria.
authors.forename: Julia
authors.initials: J
authors.lastname: Fries
authors.affiliation: AIT Austrian Institute of Technology GmbH, Bioresources Unit, Tulln, Austria.
authors.forename: Stefan
authors.initials: S
authors.lastname: Pfeiffer
authors.affiliation: AIT Austrian Institute of Technology GmbH, Bioresources Unit, Tulln, Austria.
authors.forename: Melanie
authors.initials: M
authors.lastname: Kuffner
authors.affiliation: AIT Austrian Institute of Technology GmbH, Bioresources Unit, Tulln, Austria.
authors.forename: Angela
authors.initials: A
authors.lastname: Sessitsch
mesh_terms: D001317:Austria; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002104:Cadmium / Q000378:metabolism; D041964:Cytophagaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D032108:Salix / Q000378:metabolism / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry; D015032:Zinc / Q000378:metabolism
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D002104:Cadmium; D024482:Vitamin K 2; C062629:menaquinone 7; D015032:Zinc
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