title: Gene expression profiling of bovine bronchial epithelial cells exposed in vitro to bovine herpesvirus 1 and Mannheimia haemolytica.
abstract: Bovine respiratory disease (BRD) often occurs when active respiratory virus infections (BHV-1, etc.) impair resistance to Mannheimia haemolytica infection in the lower respiratory tract. The interactions that occur when the respiratory epithelium encounters these viral and bacterial pathogens are poorly understood. We used Agilent bovine gene microarray chips containing 44,000 transcripts to elucidate bovine bronchial epithelial cell (BBEC) responses following in vitro exposure to BHV-1 alone, M. haemolytica alone, or both BHV-1 and M. haemolytica. Microarray analysis revealed differential regulation (>2-fold) of 978 transcripts by BHV-1 alone, 2040 transcripts by M. haemolytica alone, and 2189 genes by BHV-1 and M. haemolytica in combination. M. haemolytica treatment produced significantly greater inductions (>10-fold) of several inflammation associated genes, such as CXCL2, IL-6, IL-1α, e-selectin, and IL-8, than to BHV-1 alone. Functional analysis of the microarray data revealed a significant upregulation of genes involved in important biological processes such as inflammation (TNF-α, IL-8, Tlr-2, IL-1, CXCL2, CSF2), vascular functions (VEGF, EDN2) and leukocyte migration (ICAM1, IL-16) during a co-infection with BHV-1 and M. haemolytica compared to either pathogen alone. This study provides evidence to support that lung epithelial cells are a source of mediators that may promote inflammatory changes observed during bovine respiratory disease.
authors.affiliation: Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, 2015 Linden Drive-West, WI 52706, United States.
authors.forename: Alhaji U
authors.initials: AU
authors.lastname: N'jai
authors.forename: Jose
authors.initials: J
authors.lastname: Rivera
authors.forename: Dhammika N
authors.initials: DN
authors.lastname: Atapattu
authors.forename: Kwadwo
authors.initials: K
authors.lastname: Owusu-Ofori
authors.forename: Charles J
authors.initials: CJ
authors.lastname: Czuprynski
mesh_terms: D000818:Animals; D001980:Bronchi / Q000378:metabolism*; D002417:Cattle; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000378:metabolism; D020869:Gene Expression Profiling; D007242:Herpesvirus 1, Bovine / Q000472:pathogenicity*; D016978:Mannheimia haemolytica / Q000472:pathogenicity*; D020411:Oligonucleotide Array Sequence Analysis; D016133:Polymerase Chain Reaction
keywords: Bovine herpesvirus 1; Bronchial epithelial cells; Mannheimia haemolytica; Microarray
references.citation: Vet Immunol Immunopathol. 2005 Aug 15;107(1-2):119-30
references.pmid: 15979157
references.citation: Vet Immunol Immunopathol. 2005 May 15;105(3-4):277-87
references.pmid: 15808306
references.citation: New Microbiol. 2002 Oct;25(4):427-36
references.pmid: 12437222
references.citation: Vet Immunol Immunopathol. 2004 Jun;99(3-4):193-202
references.pmid: 15135985
references.citation: Infect Immun. 2002 Aug;70(8):4336-43
references.pmid: 12117943
references.citation: Vet Immunol Immunopathol. 1988 Oct;19(3-4):273-84
references.pmid: 2855289
references.citation: Clin Sci (Lond). 1993 Dec;85(6):753-60
references.pmid: 8287669
references.citation: J Cardiovasc Dis Res. 2010 Jan;1(1):29-36
references.pmid: 21188088
references.citation: Infect Immun. 2009 Apr;77(4):1426-41
references.pmid: 19139196
references.citation: Lancet. 2004 Jan 3;363(9402):62-4
references.pmid: 14723997
references.citation: Infect Immun. 2000 Jul;68(7):3916-22
references.pmid: 10858203
references.citation: Br J Pharmacol. 2000 Jan;129(1):170-6
references.pmid: 10694217
references.citation: Virus Res. 1998 Jan;53(1):91-6
references.pmid: 9617772
references.citation: Am J Pathol. 1991 May;138(5):1191-8
references.pmid: 2024707
references.citation: Vet Immunol Immunopathol. 2009 Oct 15;131(3-4):167-76
references.pmid: 19406483
references.citation: Comp Funct Genomics. 2005;6(4):244-50
references.pmid: 18629190
references.citation: Vet Pathol. 2001 May;38(3):297-310
references.pmid: 11355660
references.citation: Cancer Metastasis Rev. 2006 Sep;25(3):373-85
references.pmid: 17006765
references.citation: Clin Immunol Immunopathol. 1991 Aug;60(2):153-70
references.pmid: 1649026
references.citation: Infect Immun. 2009 Jan;77(1):446-55
references.pmid: 18981250
references.citation: Infect Immun. 1997 Jul;65(7):2593-8
references.pmid: 9199425
references.citation: Expert Rev Gastroenterol Hepatol. 2010 Dec;4(6):723-31
references.pmid: 21108592
references.citation: J Am Vet Med Assoc. 2001 Oct 15;219(8):1122-7
references.pmid: 11700712
references.citation: Am J Vet Res. 2001 Jan;62(1):136-41
references.pmid: 11197552
references.citation: Infect Immun. 1982 Mar;35(3):1048-57
references.pmid: 6175574
references.citation: Infect Immun. 2000 Jul;68(7):4274-81
references.pmid: 10858245
references.citation: J Gen Virol. 1994 Sep;75 ( Pt 9):2303-9
references.pmid: 8077928
references.citation: Vet Immunol Immunopathol. 2005 Feb 10;103(3-4):187-93
references.pmid: 15621305
references.citation: Vet Microbiol. 1996 Nov;53(1-2):31-42
references.pmid: 9010996
references.citation: Am J Vet Res. 1990 Oct;51(10):1616-22
references.pmid: 2173445
references.citation: Can J Comp Med. 1982 Jul;46(3):225-63
references.pmid: 6290011
references.citation: Vet Immunol Immunopathol. 1984 Jan;5(3):259-71
references.pmid: 6719822
references.citation: J Infect Dis. 1985 May;151(5):937-47
references.pmid: 2580916