title: DNA methylation profiles provide a viable index for porcine pluripotent stem cells.
abstract: Porcine induced pluripotent stem cells (iPSCs) provide useful information for translational research. The quality of iPSCs can be assessed by their ability to differentiate into various cell types after chimera formation. However, analysis of chimera formation in pigs is a labor-intensive and costly process, necessitating a simple evaluation method for porcine iPSCs. Our previous study identified mouse embryonic stem cell (ESC)-specific hypomethylated loci (EShypo-T-DMRs), and, in this study, 36 genes selected from these were used to evaluate porcine iPSC lines. Based on the methylation profiles of the 36 genes, the iPSC line, Porco Rosso-4, was found closest to mouse pluripotent stem cells among 5 porcine iPSCs. Moreover, Porco Rosso-4 more efficiently contributed to the inner cell mass (ICM) of blastocysts than the iPSC line showing the lowest reprogramming of the 36 genes (Porco Rosso-622-14), indicating that the DNA methylation profile correlates with efficiency of ICM contribution. Furthermore, factors known to enhance iPSC quality (serum-free medium with PD0325901 and CHIR99021) improved the methylation status at the 36 genes. Thus, the DNA methylation profile of these 36 genes is a viable index for evaluation of porcine iPSCs.
authors.affiliation: Department of Life Sciences, Laboratory of Developmental Engineering, School of Agriculture, Meiji University, Kanagawa, Japan.
authors.forename: Yoshikazu
authors.initials: Y
authors.lastname: Arai
authors.forename: Jun
authors.initials: J
authors.lastname: Ohgane
authors.forename: Shuh-hei
authors.initials: SH
authors.lastname: Fujishiro
authors.forename: Kazuaki
authors.initials: K
authors.lastname: Nakano
authors.forename: Hitomi
authors.initials: H
authors.lastname: Matsunari
authors.forename: Masahito
authors.initials: M
authors.lastname: Watanabe
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Umeyama
authors.forename: Dai
authors.initials: D
authors.lastname: Azuma
authors.forename: Naomi
authors.initials: N
authors.lastname: Uchida
authors.forename: Nozomu
authors.initials: N
authors.lastname: Sakamoto
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Makino
authors.forename: Shintaro
authors.initials: S
authors.lastname: Yagi
authors.forename: Kunio
authors.initials: K
authors.lastname: Shiota
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Hanazono
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Nagashima
mesh_terms: D000818:Animals; D053624:Blastocyst Inner Cell Mass / Q000378:metabolism; D002460:Cell Line; D002678:Chimera; D019175:DNA Methylation; D004622:Embryo, Mammalian; D053595:Embryonic Stem Cells / Q000378:metabolism*; D015870:Gene Expression; D005796:Genes; D056426:Genetic Loci; D057026:Induced Pluripotent Stem Cells / Q000378:metabolism*; D051379:Mice; D013552:Swine / Q000196:embryology*; D013556:Swine, Miniature / Q000196:embryology*; D057170:Translational Research, Biomedical
keywords: epigenetics; induced pluripotent stem cells; translational research
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