title: Abundance and diversity of archaeal accA gene in hot springs in Yunnan Province, China.
abstract: It has been suggested that archaea carrying the accA gene, encoding the alpha subunit of the acetyl CoA carboxylase, autotrophically fix CO2 using the 3-hydroxypropionate/4-hydroxybutyrate pathway in low-temperature environments (e.g., soils, oceans). However, little new information has come to light regarding the occurrence of archaeal accA genes in high-temperature ecosystems. In this study, we investigated the abundance and diversity of archaeal accA gene in hot springs in Yunnan Province, China, using DNA- and RNA-based phylogenetic analyses and quantitative polymerase chain reaction. The results showed that archaeal accA genes were present and expressed in the investigated Yunnan hot springs with a wide range of temperatures (66-96 °C) and pH (4.3-9.0). The majority of the amplified archaeal accA gene sequences were affiliated with the ThAOA/HWCG III [thermophilic ammonia-oxidizing archaea (AOA)/hot water crenarchaeotic group III]. The archaeal accA gene abundance was very close to that of AOA amoA gene, encoding the alpha subunit of ammonia monooxygenase. These data suggest that AOA in terrestrial hot springs might acquire energy from ammonia oxidation coupled with CO2 fixation using the 3-hydroxypropionate/4-hydroxybutyrate pathway.
authors.affiliation: Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education, and Laboratory for Conservation and Utilization of Bio-resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, 650091, China.
authors.forename: Zhao-Qi
authors.initials: ZQ
authors.lastname: Song
authors.forename: Li
authors.initials: L
authors.lastname: Wang
authors.forename: Feng-Ping
authors.initials: FP
authors.lastname: Wang
authors.forename: Hong-Chen
authors.initials: HC
authors.lastname: Jiang
authors.forename: Jin-Quan
authors.initials: JQ
authors.lastname: Chen
authors.forename: En-Min
authors.initials: EM
authors.lastname: Zhou
authors.forename: Feng
authors.initials: F
authors.lastname: Liang
authors.forename: Xiang
authors.initials: X
authors.lastname: Xiao
authors.forename: Wen-Jun
authors.initials: WJ
authors.lastname: Li
mesh_terms: D000103:Acetyl-CoA Carboxylase / Q000235:genetics*; D001105:Archaea / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D019843:Archaeal Proteins / Q000235:genetics*; D002681:China; D019766:Genes, Archaeal; D045482:Hot Springs / Q000382:microbiology*; D064307:Microbiota; D010088:Oxidoreductases / Q000235:genetics; D010802:Phylogeny
chemical_list: D019843:Archaeal Proteins; D010088:Oxidoreductases; C045582:ammonia monooxygenase; D000103:Acetyl-CoA Carboxylase
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