title: Use of a new generation of capillary electrophoresis to quantify circulating free DNA in non-small cell lung cancer.
abstract: Circulating free DNA (cfDNA) is present in higher concentration in non-small-cell lung cancer (NSCLC) patients than in controls. This study was designed to assess the sensitivity and specificity of Agilent 2100 Bioanalyzer to identify patients with NSCLC and to compare it with quantitative RealTime-PCR (RT-qPCR) assay. 30 NSCLC patients and 26 controls were analyzed. The amount of cfDNA was determined both through quantitative RT-PCR targeting the human β-actin gene and by Agilent 2100 Bioanalyzer. Performances of the assays were calculated by the receiver operating characteristic (ROC) curves. The mean cfDNA concentration, obtained through the use of Agilent 2100 Bioanalyzer, in NSCLC patients (94.5 ng/mL) was almost twice the concentration detected in controls (42.8 ng/mL) as well as found by RT-qPCR (22.5 ng/mL vs 7.1 ng/mL, p<0.001). The area under curve of Agilent 2100 Bioanalyzer and RT-PCR showed that there are no statistically significant differences between these tests (p<0.92). This study shows that Agilent 2100 Bioanalyzer is an effective diagnostic tool to discriminate NSCLC patients from healthy individuals and suggests a new approach for early detection of NSCLC.
authors.affiliation: Department of Medico-Surgical Sciences and Biotechnologies, Faculty of Medicine and Pharmacy, Sapienza University, Polo Pontino, Corso Della Repubblica 79, Latina, Italy.
authors.forename: Caterina
authors.initials: C
authors.lastname: Chiappetta
authors.forename: Marco
authors.initials: M
authors.lastname: Anile
authors.forename: Martina
authors.initials: M
authors.lastname: Leopizzi
authors.forename: Federico
authors.initials: F
authors.lastname: Venuta
authors.forename: Carlo
authors.initials: C
authors.lastname: Della Rocca
mesh_terms: D000199:Actins / Q000097:blood / Q000235:genetics; D000328:Adult; D000368:Aged; D019540:Area Under Curve; D057205:Automation, Laboratory; D014408:Biomarkers, Tumor / Q000097:blood*; D002289:Carcinoma, Non-Small-Cell Lung / Q000097:blood* / Q000175:diagnosis / Q000235:genetics / Q000601:surgery; D016022:Case-Control Studies; D004247:DNA / Q000097:blood*; D042241:Early Diagnosis; D019075:Electrophoresis, Capillary; D005260:Female; D015870:Gene Expression; D006801:Humans; D008175:Lung Neoplasms / Q000097:blood* / Q000175:diagnosis / Q000235:genetics / Q000601:surgery; D008297:Male; D008875:Middle Aged; D012372:ROC Curve; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D000199:Actins; D014408:Biomarkers, Tumor; D004247:DNA
keywords: Circulating free DNA; Early diagnosis; Non-small cell lung cancer