title: Label free redox capacitive biosensing.
abstract: A surface confined redox group contributes to an interfacial charging (quantifiable by redox capacitance) that can be sensitively probed by impedance derived capacitance spectroscopy. In generating mixed molecular films comprising such redox groups, together with specific recognition elements (here antibodies), this charging signal is able to sensitively transduce the recognition and binding of specific analytes. This novel transduction method, exemplified here with C-reactive protein, an important biomarker of cardiac status and general trauma, is equally applicable to any suitably prepared interfacial combination of redox reporter and receptor. The assays are label free, ultrasensitive, highly specific and accompanied by a good linear range.
authors.affiliation: Instituto de Química, Universidade Estadual Paulista, CP 355, 14800-900 Araraquara, São Paulo, Brazil.
authors.forename: Flávio C Bedatty
authors.initials: FC
authors.lastname: Fernandes
authors.forename: Márcio S
authors.initials: MS
authors.lastname: Góes
authors.forename: Jason J
authors.initials: JJ
authors.lastname: Davis
authors.forename: Paulo R
authors.initials: PR
authors.lastname: Bueno
mesh_terms: D055910:Antibodies, Immobilized / Q000737:chemistry; D015374:Biosensing Techniques / Q000379:methods*; D002097:C-Reactive Protein / Q000032:analysis*; D025802:Electric Capacitance; D007118:Immunoassay / Q000379:methods; D057230:Limit of Detection; D008958:Models, Molecular; D010084:Oxidation-Reduction; D013057:Spectrum Analysis
chemical_list: D055910:Antibodies, Immobilized; D002097:C-Reactive Protein
keywords: Biosensor; Capacitance spectroscopy; Immunosensors; Redox capacitance