title: [Isolation and characterization of a human enterovirus 74 isolated in Shandong Province, China].
abstract: Human Enterovirus HEV 74 is a new member of species Human enterovirus B (HEV-B). To understand its evolution and restructuring characteristics, we report the complete genome sequence of a HEV74 strain 05293/SD/CHN/2005(abbreviated as 05293) isolated from an acute flaccid paralysis (AFP) case in Shangdong Province, China, 2005. Analysis of the complete genomic sequence of 05293 showed that its genome was collinear with that of previously described 2 HEV74 strains, except for insertions and deletions at the 5'NTR and the 3 NTR regions. The complete genome sequence of strain 05293 displayed 80. 8% nucleotide and 96% amino acid identity to the prototype strain USA/CA75-10213, and 80. 6% and 95. 9% to another isolated strain Rikaze-136. The P1, P2 and P3 coding regions of strain 05293 displayed 81. 5%, 80. 0%, 79. 7% nucleotide and 95. 9%, 96. 0%, 96.2% amino acid identity to the prototype strain USA/CA75-10213, and 81. 9%, 78. 8%, 79. 5% and 95. 9%, 96. 1%, 95. 7% to strain Rikaze-136, respectively. The phylogenetic tree and Simplot analysis on 05293 and HEV-B genome sequences were performed, and the result indicated frequent recombination within HEV-B.
authors.affiliation: School of Public Health of Shandong University, Jinan 250012, China. 190165446@qq.com
authors.forename: Xiao-Lin
authors.initials: XL
authors.lastname: Liu
authors.forename: Ze-Xin
authors.initials: ZX
authors.lastname: Tao
authors.forename: Hai-Yan
authors.initials: HY
authors.lastname: Wang
authors.forename: Yao
authors.initials: Y
authors.lastname: Liu
authors.forename: Li-Zhi
authors.initials: LZ
authors.lastname: Song
authors.forename: Feng
authors.initials: F
authors.lastname: Ji
authors.forename: Tong-Zhan
authors.initials: TZ
authors.lastname: Wang
authors.forename: Xiao-Juan
authors.initials: XJ
authors.lastname: Lin
authors.forename: Su-Ting
authors.initials: ST
authors.lastname: Wang
authors.forename: Ai-Qiang
authors.initials: AQ
authors.lastname: Xu
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics; D020121:5' Untranslated Regions / Q000235:genetics; D001483:Base Sequence; D002681:China; D029822:Enterovirus B, Human / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D004769:Enterovirus Infections / Q000821:virology*; D019143:Evolution, Molecular; D016679:Genome, Viral / Q000235:genetics*; D006801:Humans; D009123:Muscle Hypotonia; D010243:Paralysis / Q000821:virology*; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics; D011995:Recombination, Genetic; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
chemical_list: D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D012367:RNA, Viral