title: Diphlorethohydroxycarmalol attenuated cell damage against UVB radiation via enhancing antioxidant effects and absorbing UVB ray in human HaCaT keratinocytes.
abstract: Exposure of human skin to excessive ultraviolet B (UVB) radiation induces pathophysiological processes via the generation of reactive oxygen species (ROS) in skin cells, such as keratinocytes. This study investigated the ability of diphlorethohydroxycarmalol (DPHC) to protect human keratinocytes (HaCaT) against UVB-induced cell damage. DPHC restored cell viability that was reduced by UVB light. DPHC had an absorption maximum close to the UVB spectrum and decreased UVB-induced intracellular ROS levels, increased levels of reduced glutathione, activated superoxide dismutase and catalase. DPHC also decreased UVB-mediated damage to cellular components, including lipids, proteins, DNA, and attenuated UVB-induced apoptosis. These results suggest that DPHC safeguards human keratinocytes against UVB-induced cell damage by absorbing UVB ray, scavenging ROS and enhancing antioxidant system.
authors.affiliation: School of Medicine, Jeju National University, Jeju 690-756, Republic of Korea.
authors.forename: Mei Jing
authors.initials: MJ
authors.lastname: Piao
authors.affiliation: School of Medicine, Jeju National University, Jeju 690-756, Republic of Korea.
authors.forename: Kyoung Ah
authors.initials: KA
authors.lastname: Kang
authors.affiliation: School of Medicine, Jeju National University, Jeju 690-756, Republic of Korea.
authors.forename: Ki Cheon
authors.initials: KC
authors.lastname: Kim
authors.affiliation: Aging Research Center, Korea Institute of Oriental Medicine, Daejeon 305-811, Republic of Korea.
authors.forename: Sungwook
authors.initials: S
authors.lastname: Chae
authors.affiliation: Jeju Technopark Bioconvergence Center, Jeju 690-121, Republic of Korea.
authors.forename: Gi Ok
authors.initials: GO
authors.lastname: Kim
authors.affiliation: Laboratory of Veterinary Anatomy, College of Veterinary Medicine, Jeju National University, Jeju 690-756, Republic of Korea.
authors.forename: Taekyun
authors.initials: T
authors.lastname: Shin
authors.affiliation: Cancer Research Institute, Seoul National University College of Medicine, Seoul 110-799, Republic of Korea.
authors.forename: Hye Sun
authors.initials: HS
authors.lastname: Kim
authors.affiliation: School of Medicine, Jeju National University, Jeju 690-756, Republic of Korea. Electronic address: jinwonh@jejunu.ac.kr.
authors.forename: Jin Won
authors.initials: JW
authors.lastname: Hyun
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects / Q000528:radiation effects; D002374:Catalase / Q000378:metabolism; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D019610:Cytoprotection; D004249:DNA Damage / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005978:Glutathione / Q000378:metabolism; D006575:Heterocyclic Compounds, 3-Ring / Q000494:pharmacology*; D006801:Humans; D015603:Keratinocytes / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology / Q000528:radiation effects*; D018384:Oxidative Stress / Q000187:drug effects* / Q000528:radiation effects*; D017382:Reactive Oxygen Species / Q000378:metabolism; D013473:Sunscreening Agents / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000378:metabolism; D014466:Ultraviolet Rays / Q000009:adverse effects*
chemical_list: D000975:Antioxidants; D006575:Heterocyclic Compounds, 3-Ring; D017382:Reactive Oxygen Species; D013473:Sunscreening Agents; C513811:diphlorethohydroxycarmalol; D002374:Catalase; D013482:Superoxide Dismutase; D005978:Glutathione
keywords: Antioxidant system; Diphlorethohydroxycarmalol; Oxidative stress; Reactive oxygen species; Ultraviolet B absorption