title: A computational workflow to identify allele-specific expression and epigenetic modification in maize.
abstract: Allele-specific expression refers to the preferential expression of one of the two alleles in a diploid genome, which has been thought largely attributable to the associated cis-element variation and allele-specific epigenetic modification patterns. Allele-specific expression may contribute to the heterosis (or hybrid vigor) effect in hybrid plants that are produced from crosses of closely-related species, subspecies and/or inbred lines. In this study, using Illumina high-throughput sequencing of maize transcriptomics, chromatic H3K27me3 histone modification and DNA methylation data, we developed a new computational framework to identify allele-specifically expressed genes by simultaneously tracking allele-specific gene expression patterns and the epigenetic modification landscape in the seedling tissues of hybrid maize. This approach relies on detecting nucleotide polymorphisms and any genomic structural variation between two parental genomes in order to distinguish paternally or maternally derived sequencing reads. This computational pipeline also incorporates a modified Chi-square test to statistically identify allele-specific gene expression and epigenetic modification based on the Poisson distribution.
authors.affiliation: Department of Basic Medicine, Medical College of Qinghai University, Xining 810016, China.
authors.forename: Xiaoxing
authors.initials: X
authors.lastname: Wei
authors.forename: Xiangfeng
authors.initials: X
authors.lastname: Wang
mesh_terms: D000483:Alleles; D019175:DNA Methylation; D044127:Epigenesis, Genetic; D057890:Epigenomics; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D018745:Genome, Plant; D012985:Software Design; D059467:Transcriptome; D057188:Workflow; D003313:Zea mays / Q000235:genetics* / Q000378:metabolism
keywords: Allele-specific expression; Epigenetic modification; Hybrid maize; Illumina sequencing; Poisson model
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