title: High throughput screening to investigate the interaction of stem cells with their extracellular microenvironment.
abstract: Stem cells in vivo are housed within a functional microenvironment termed the "stem cell niche." As the niche components can modulate stem cell behaviors like proliferation, migration and differentiation, evaluating these components would be important to determine the most optimal platform for their maintenance or differentiation. In this review, we have discussed methods and technologies that have aided in the development of high throughput screening assays for stem cell research, including enabling technologies such as the well-established multiwell/microwell plates and robotic spotting, and emerging technologies like microfluidics, micro-contact printing and lithography. We also discuss the studies that utilized high throughput screening platform to investigate stem cell response to extracellular matrix, topography, biomaterials and stiffness gradients in the stem cell niche. The combination of the aforementioned techniques could lay the foundation for new perspectives in further development of high throughput technology and stem cell research.
authors.affiliation: Department of Bioengineering; National University of Singapore; Singapore; Duke-NUS Graduate Medical School; Singapore.
authors.forename: Soneela
authors.initials: S
authors.lastname: Ankam
authors.forename: Benjamin K K
authors.initials: BK
authors.lastname: Teo
authors.forename: Marek
authors.initials: M
authors.lastname: Kukumberg
authors.forename: Evelyn K F
authors.initials: EK
authors.lastname: Yim
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002478:Cells, Cultured; D005109:Extracellular Matrix; D057166:High-Throughput Screening Assays; D006801:Humans; D051379:Mice; D051381:Rats; D055153:Stem Cell Niche; D057905:Stem Cell Research; D013234:Stem Cells
keywords: biomaterials; biotechnology; differentiation; extracellular matrix; maintenance; microarray; niche; proliferation; stiffness; topography
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