title: Serum concentrations of 25-hydroxyvitamin D and immunoglobulins in an older Swiss cohort: results of the Senior Labor Study.
abstract: BACKGROUND
  Vitamin D and the components of humoral immunity play important roles in human health. Older people have lower 25-hydroxyvitamin D (25(OH)D) serum levels than younger adults. We aimed to determine the levels of 25(OH)D serum concentrations in healthy senior citizens and to study their relationship to the levels of components of humoral immunity.
  
  
  METHODS
  A total of 1,470 healthy Swiss men and women, 60 years or older, were recruited for this study. A total of 179 subjects dropped out of the study because of elevated serum concentrations of C-reactive protein. Fasting blood sera were analyzed for 25(OH)D with the high-performance liquid chromatography (HPLC) and for parathyroid hormone (PTH), immunoglobulins and complement C4 and C3 concentrations with immunoassays. The percentage of participants in each of the four 25(OH)D deficiency groups--severely deficient (<10 ng/ml), deficient (10 to 20), insufficient (21 to 29 ng/ml) and normal (>=30 ng/ml)--were statistically compared. The relationship of the major components of the humoral system and age with 25(OH)D levels was also assessed.
  
  
  RESULTS
  About 66% of the subjects had insufficient levels of 25(OH)D. Normal levels of 25(OH)D were found in 26.1% of the subjects of which 21% were males and 30.5% were females (total study population). Severely deficient levels of 25(OH)D were found in 7.98% of the total study population. Low levels of 25(OH)D were positively associated with IgG2 (P = 0.01) and with C4 (P = 0.02), yet were inversely related to levels of IgG1 and IgA (P < 0.05) and C3 (P = 0.01). Serum levels of total IgA, IgG, IgG2 and IgG4 peaked together with 25(OH)D during late summer.
  
  
  CONCLUSIONS
  Approximately two-thirds of the healthy, older Swiss population presented with Vitamin D insufficiency. The incremental shift in IgA and C3 levels might not necessarily reflect a deranged humoral immune defense; however, given the high prevalence of vitamin D deficiency, the importance of this condition in humoral immunity will be worth looking at more closely. This study supports the role of vitamin D in the competent immune system.
authors.affiliation: Division of Clinical Chemistry, Labormedizinisches Zentrum Dr Risch, Waldeggstrasse 37, CH-3097 Liebefeld bei Bern, Switzerland.
authors.forename: Benjamin
authors.initials: B
authors.lastname: Sakem
authors.forename: Cristina
authors.initials: C
authors.lastname: Nock
authors.forename: Zeno
authors.initials: Z
authors.lastname: Stanga
authors.forename: Pedro
authors.initials: P
authors.lastname: Medina
authors.forename: Urs E
authors.initials: UE
authors.lastname: Nydegger
authors.forename: Martin
authors.initials: M
authors.lastname: Risch
authors.forename: Lorenz
authors.initials: L
authors.lastname: Risch
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D015415:Biomarkers / Q000097:blood; D015331:Cohort Studies; D005260:Female; D006801:Humans; D007136:Immunoglobulins / Q000096:biosynthesis / Q000097:blood*; D008297:Male; D008875:Middle Aged; D013557:Switzerland / Q000453:epidemiology; D014807:Vitamin D / Q000031:analogs & derivatives* / Q000097:blood; D014808:Vitamin D Deficiency / Q000097:blood* / Q000175:diagnosis / Q000453:epidemiology*
chemical_list: D015415:Biomarkers; D007136:Immunoglobulins; D014807:Vitamin D; C104450:25-hydroxyvitamin D
references.citation: J Clin Endocrinol Metab. 2012 Oct;97(10):3717-23
references.pmid: 22767638
references.citation: J Immunol. 2007 Aug 1;179(3):1634-47
references.pmid: 17641030
references.citation: J Clin Endocrinol Metab. 2009 Apr;94(4):1214-22
references.pmid: 19174492
references.citation: Proc Natl Acad Sci U S A. 2011 Nov 22;108(47):19013-7
references.pmid: 22025704
references.citation: J Nephrol. 2011 May-Jun;24 Suppl 18:S30-7
references.pmid: 21623580
references.citation: Dtsch Arztebl Int. 2012 Jan;109(3):33-8
references.pmid: 22334819
references.citation: Nat Med. 2012 Jan 29;18(2):267-73
references.pmid: 22286305
references.citation: Minerva Endocrinol. 2011 Sep;36(3):257-66
references.pmid: 22019754
references.citation: Mol Cell Endocrinol. 2010 Jun 10;321(2):103-11
references.pmid: 20156523
references.citation: J Steroid Biochem Mol Biol. 2010 Jul;121(1-2):314-6
references.pmid: 20138991
references.citation: Curr Osteoporos Rep. 2012 Mar;10(1):4-15
references.pmid: 22249582
references.citation: Isr Med Assoc J. 2010 Dec;12(12):751-6
references.pmid: 21348404
references.citation: Clin Chem. 2012 Mar;58(3):531-42
references.pmid: 22230812
references.citation: J Immunol. 2013 Apr 1;190(7):3687-95
references.pmid: 23436936
references.citation: Acta Neurol Scand. 2012 Jun;125(6):e28-31
references.pmid: 21781056
references.citation: J Immunol. 2009 Apr 1;182(7):4296-305
references.pmid: 19299729
references.citation: J Clin Endocrinol Metab. 2011 Jul;96(7):1931-42
references.pmid: 21677037
references.citation: Swiss Med Wkly. 2012 Sep 04;142:w13672
references.pmid: 22949267
references.citation: Clin Chem. 2010 May;56(5):729-31
references.pmid: 20348405
references.citation: Hum Genet. 2010 Feb;127(2):207-21
references.pmid: 19902255
references.citation: J Clin Endocrinol Metab. 2011 Jul;96(7):1911-30
references.pmid: 21646368
references.citation: Ann Intern Med. 2011 Dec 20;155(12):820-6
references.pmid: 22184689
references.citation: Prostate. 2011 Mar 1;71(4):368-72
references.pmid: 20812224
references.citation: J Clin Endocrinol Metab. 2012 Apr;97(4):1153-8
references.pmid: 22442274
references.citation: Am J Clin Nutr. 2003 Jan;77(1):204-10
references.pmid: 12499343
references.citation: Hepatology. 2012 Dec;56(6):2180-7
references.pmid: 22753133
references.citation: Nat Rev Immunol. 2008 Sep;8(9):685-98
references.pmid: 19172691
references.citation: Mayo Clin Proc. 2010 Aug;85(8):752-7; quiz 757-8
references.pmid: 20675513
references.citation: Clin Interv Aging. 2013;8:509-14
references.pmid: 23667311
references.citation: Adv Prev Med. 2011;2011:281863
references.pmid: 21991434
references.citation: PLoS One. 2011 Jan 31;6(1):e16709
references.pmid: 21304967
references.citation: J Clin Endocrinol Metab. 2012 Apr;97(4):1146-52
references.pmid: 22442278
references.citation: J Clin Invest. 1984 Aug;74(2):657-61
references.pmid: 6611355
references.citation: Immun Ageing. 2013 Apr 22;10(1):14
references.pmid: 23607926
references.citation: N Engl J Med. 2007 Jul 19;357(3):266-81
references.pmid: 17634462
references.citation: J Clin Virol. 2011 Mar;50(3):194-200
references.pmid: 21242105
references.citation: Arch Intern Med. 2009 Mar 23;169(6):626-32
references.pmid: 19307527
references.citation: Immunology. 1992 Dec;77(4):520-6
references.pmid: 1493924
references.citation: Aging Clin Exp Res. 2012 Oct;24(5):548-51
references.pmid: 22314303
references.citation: N Engl J Med. 2012 Jul 5;367(1):40-9
references.pmid: 22762317
references.citation: Curr Osteoporos Rep. 2009 Jul;7(2):58-63
references.pmid: 19631030
references.citation: Aging Cell. 2011 Dec;10(6):922-30
references.pmid: 21726404
references.citation: Arch Intern Med. 2007 Jun 11;167(11):1159-65
references.pmid: 17563024
references.citation: Vaccine. 2012 Jan 20;30(5):931-5
references.pmid: 22142584
references.citation: N Engl J Med. 2011 Jan 20;364(3):248-54
references.pmid: 21247315
references.citation: Nat Immunol. 2007 Mar;8(3):285-93
references.pmid: 17259988
references.citation: Ann N Y Acad Sci. 2013 May;1287:45-58
references.pmid: 23682710
references.citation: Proc Natl Acad Sci U S A. 2010 Dec 28;107(52):22593-8
references.pmid: 21149724
references.citation: Osteoporos Int. 2011 Jul;22(7):2107-18
references.pmid: 21207011
references.citation: Eur J Clin Nutr. 2011 Jan;65(1):102-9
references.pmid: 20859300
references.citation: J Leukoc Biol. 2012 May;91(5):829-38
references.pmid: 22345707
references.citation: Autoimmun Rev. 2011 Apr;10(6):317-24
references.pmid: 21156217