title: Serum samples can be substituted by plasma samples for the diagnosis of paratuberculosis.
abstract: Employing plasma samples rather than serum samples for serological paratuberculosis diagnosis is practical, especially when bovine TB is assessed in the same cattle herd with the gamma interferon bovine avian (IFN-γ BA) test. We demonstrate that antibody titers in serum and plasma samples, utilizing the PARACHECK(®) ELISA kit, are highly comparable (Cohen's kappa test, k=0.955). We conclude that serum can be replaced with plasma in this commercially available antibody detection assay resulting in working hour savings for sampling and blood sample work-up and cost reductions for materials and sample storage.
authors.affiliation: Instituto de Investigaciones Científicas y Servicios de Alta Tecnología INDICASAT-AIP, City of Knowledge, Panama. amadorgj@yahoo.com
authors.forename: Amador
authors.initials: A
authors.lastname: Goodridge
authors.forename: Ricardo
authors.initials: R
authors.lastname: Correa
authors.forename: Paul
authors.initials: P
authors.lastname: Castro
authors.forename: Cecilia
authors.initials: C
authors.lastname: Escobar
authors.forename: Jacobus H
authors.initials: JH
authors.lastname: de Waard
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis* / Q000382:microbiology; D004797:Enzyme-Linked Immunosorbent Assay / Q000191:economics / Q000379:methods* / Q000662:veterinary; D005260:Female; D016927:Mycobacterium avium subsp. paratuberculosis / Q000302:isolation & purification*; D010176:Panama; D010283:Paratuberculosis / Q000175:diagnosis* / Q000382:microbiology; D010949:Plasma / Q000382:microbiology*; D044967:Serum / Q000382:microbiology*
chemical_list: D000907:Antibodies, Bacterial
keywords: Biomarker; Bovine tuberculosis; Diagnosis; ELISA; PARACHECK(®); Paratuberculosis