title: Superresolution by image scanning microscopy using pixel reassignment.
abstract: The effect of detector array size on resolution and signal collection efficiency of image scanning microscopy based on pixel reassignment is studied. It is shown how the method can also be employed if there is a Stokes shift in fluorescence emission wavelength. With no Stokes shift, the width of the point spread function can be sharpened by a factor of 1.53, and its peak intensity increased by a factor of 1.84.
authors.affiliation: Nanophysics, Istituto Italiano di Tecnologia, Genova, Italy. colinjrsheppard@gmail.com
authors.forename: Colin J R
authors.initials: CJ
authors.lastname: Sheppard
authors.forename: Shalin B
authors.initials: SB
authors.lastname: Mehta
authors.forename: Rainer
authors.initials: R
authors.lastname: Heintzmann
mesh_terms: D005456:Fluorescent Dyes; D008853:Microscopy / Q000379:methods*
chemical_list: D005456:Fluorescent Dyes