title: Use of thiamine pyrophosphate to prevent infertility developing in rats undergoing unilateral ovariectomy and with ischemia reperfusion induced in the contralateral ovary.
abstract: OBJECTIVE
  To investigate whether thiamine pyrophosphate can prevent infertility developing in rats undergoing unilateral ovariectomy and with ischemia reperfusion induced in the contralateral ovary. Biochemical examinations of the ovaries were also performed.
  
  
  METHODS
  Rats were divided into two main groups of three subgroups each. An ischemia reperfusion model was established in the first main group, while surgical unilateral ovariectomy was performed in the second. Thiamine pyrophosphate and melatonin were administered to the subgroups. No additional procedure was performed in the control groups. The rats were then left in laboratory environments and their fertility levels were determined. Malondialdehyde, total glutathione and DNA damage products were measured in those rats from which ovarian tissue was collected.
  
  
  RESULTS
  The results showed that thiamine pyrophosphate prevented ischemia/reperfusion injury-related infertility, but melatonin did not provide adequate prevention. However, reproduction in healthy animals receiving melatonin began earlier compared to those receiving thiamine pyrophosphate. Melatonin suppressed oxidative stress caused by ischemia/reperfusion in ovarian tissue significantly better than did thiamine pyrophosphate.
  
  
  CONCLUSIONS
  We think that different mechanisms, in addition to antioxidant activity, are involved in the prevention of reperfusion-associated infertility after ischemia.
authors.affiliation: Sorgun State Hospital, Obstetric and Gynecology, Yozgat, Turkey.
authors.forename: Omer E
authors.initials: OE
authors.lastname: Yapca
authors.forename: Mehmet I
authors.initials: MI
authors.lastname: Turan
authors.forename: Nihal
authors.initials: N
authors.lastname: Cetin
authors.forename: Bunyamin
authors.initials: B
authors.lastname: Borekci
authors.forename: Mehmet A
authors.initials: MA
authors.lastname: Gul
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000627:therapeutic use; D004195:Disease Models, Animal; D005260:Female; D007247:Infertility, Female / Q000209:etiology / Q000517:prevention & control*; D008550:Melatonin / Q000627:therapeutic use; D010049:Ovarian Diseases / Q000150:complications / Q000188:drug therapy*; D010052:Ovariectomy; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000150:complications / Q000517:prevention & control*; D013835:Thiamine Pyrophosphate / Q000627:therapeutic use*; D014803:Vitamin B Complex / Q000627:therapeutic use*
chemical_list: D000975:Antioxidants; D014803:Vitamin B Complex; D008550:Melatonin; D013835:Thiamine Pyrophosphate
keywords: Antioxidants; Ischemia; Ovary; Rat; Reperfusion; Thiamine pyrophosphate