title: gH625 is a viral derived peptide for effective delivery of intrinsically disordered proteins.
abstract: A genetically modified recombinant gH625-c-prune was prepared through conjugation of c-prune with gH625, a peptide encompassing 625-644 residues of the glycoprotein H of herpes simplex virus 1, which has been proved to possess the ability to carry cargo molecules across cell membranes. C-prune is the C-terminal domain of h-prune, overexpressed in breast, colorectal, and gastric cancers, interacting with multiple partners, and representing an ideal target for inhibition of cancer development. Its C-terminal domain results in an intrinsically disordered domain (IDD), and the peculiar properties of gH625 render it an optimal candidate to act as a carrier for this net negatively charged molecule by comparison with the positively charged TAT. A characterization of the recombinant gH625-c-prune fusion protein was conducted by biochemical, cellular biology and confocal microscopy means in comparison with TAT-c-prune. The results showed that the gH625-c-prune exhibited the ability to cross biomembranes, opening a new scenario on the use of gH625 as a novel multifunctional carrier.
authors.affiliation: Institute of Biostructures and Bioimaging, National Research Council, Naples, Italy.
authors.forename: Giovanni
authors.initials: G
authors.lastname: Smaldone
authors.forename: Annarita
authors.initials: A
authors.lastname: Falanga
authors.forename: Domenica
authors.initials: D
authors.lastname: Capasso
authors.forename: Daniela
authors.initials: D
authors.lastname: Guarnieri
authors.forename: Stefania
authors.initials: S
authors.lastname: Correale
authors.forename: Massimiliano
authors.initials: M
authors.lastname: Galdiero
authors.forename: Paolo A
authors.initials: PA
authors.lastname: Netti
authors.forename: Massimo
authors.initials: M
authors.lastname: Zollo
authors.forename: Stefania
authors.initials: S
authors.lastname: Galdiero
authors.forename: Sonia
authors.initials: S
authors.lastname: Di Gaetano
authors.forename: Emilia
authors.initials: E
authors.lastname: Pedone
mesh_terms: D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D002942:Circular Dichroism; D004337:Drug Carriers / Q000737:chemistry / Q000493:pharmacokinetics*; D004705:Endocytosis; D006367:HeLa Cells; D006801:Humans; D064267:Intrinsically Disordered Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D009363:Neoplasm Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D010455:Peptides / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D010744:Phosphoric Monoester Hydrolases; D017434:Protein Structure, Tertiary; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D014759:Viral Envelope Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
chemical_list: D002352:Carrier Proteins; D004337:Drug Carriers; D064267:Intrinsically Disordered Proteins; D009363:Neoplasm Proteins; D010455:Peptides; D011993:Recombinant Fusion Proteins; D014759:Viral Envelope Proteins; C044200:glycoprotein H, herpes simplex virus type 1; C438732:PRUNE1 protein, human; D010744:Phosphoric Monoester Hydrolases
keywords: IDP; delivery
references.citation: Biochemistry. 1990 Sep 11;29(36):8229-40
references.pmid: 2252886
references.citation: Biochim Biophys Acta. 2010 Mar;1798(3):579-91
references.pmid: 20085747
references.citation: Biochemistry. 1990 Oct 16;29(41):9638-43
references.pmid: 2271606
references.citation: J Biol Chem. 2005 Aug 5;280(31):28632-43
references.pmid: 15937337
references.citation: J Biol Chem. 2010 May 28;285(22):17123-36
references.pmid: 20348105
references.citation: Small. 2013 Mar 25;9(6):853-62
references.pmid: 23135878
references.citation: Ann Surg Oncol. 2009 May;16(5):1390-6
references.pmid: 17896143
references.citation: Biosci Rep. 2009 Nov 23;30(2):125-34
references.pmid: 19438410
references.citation: Protein Sci. 2005 Aug;14(8):1975-92
references.pmid: 16046624
references.citation: Trends Pharmacol Sci. 2000 Feb;21(2):45-8
references.pmid: 10664605
references.citation: Cell Mol Life Sci. 2005 Aug;62(16):1839-49
references.pmid: 15968462
references.citation: Plant Cell Physiol. 2005 Mar;46(3):482-8
references.pmid: 15695452
references.citation: BMC Cancer. 2010 Aug 24;10:458
references.pmid: 20735841
references.citation: Adv Drug Deliv Rev. 2005 Feb 28;57(4):529-45
references.pmid: 15722162
references.citation: Cancer Sci. 2007 Aug;98(8):1198-205
references.pmid: 17532757
references.citation: Mol Cell Biol. 2006 Feb;26(3):898-911
references.pmid: 16428445
references.citation: Sci Rep. 2013;3:1351
references.pmid: 23448979
references.citation: Cell Cycle. 2004 Jun;3(6):758-61
references.pmid: 15254413
references.citation: Science. 1999 Sep 3;285(5433):1569-72
references.pmid: 10477521
references.citation: PLoS One. 2012;7(6):e38372
references.pmid: 22685563
references.citation: Traffic. 2007 Jul;8(7):848-66
references.pmid: 17587406
references.citation: Nanomedicine. 2011 Dec;7(6):925-34
references.pmid: 21664490
references.citation: Clin Exp Metastasis. 2007;24(8):637-45
references.pmid: 17952613
references.citation: Biochemistry. 2012 Apr 10;51(14):3121-8
references.pmid: 22397737
references.citation: J Biol Chem. 1997 Jun 20;272(25):16010-7
references.pmid: 9188504
references.citation: Biochim Biophys Acta. 1985 Jan 10;812(1):55-65
references.pmid: 23008845
references.citation: Curr Opin Struct Biol. 2010 Oct;20(5):640-8
references.pmid: 20801639
references.citation: Biochemistry. 1998 Oct 27;37(43):15114-9
references.pmid: 9790674
references.citation: Chemistry. 2011 Nov 4;17(45):12659-68
references.pmid: 21956538
references.citation: J Biol Chem. 2008 Oct 31;283(44):29993-30009
references.pmid: 18678872
references.citation: J Biol Chem. 2002 Jan 25;277(4):2437-43
references.pmid: 11711547
references.citation: Curr Drug Metab. 2012 Jan;13(1):93-104
references.pmid: 22292812
references.citation: Cancer Cell. 2004 Feb;5(2):137-49
references.pmid: 14998490
references.citation: Mol Cell Biochem. 2009 Sep;329(1-2):149-59
references.pmid: 19390954
references.citation: Proteins. 2006 Jan 1;62(1):24-45
references.pmid: 16287116
references.citation: Chemistry. 2012 Oct 22;18(43):13678-85
references.pmid: 22968943