title: Inactivation of putative PKS genes can double geldanamycin yield in Streptomyces hygroscopicus 17997.
abstract: The putative polyketide biosynthesis (PKS) genes cos10 and pg10 were inactivated by insertion of a kanamycin-resistance gene into the genome of the geldanamycin-producing strain, Streptomyces hygroscopicus 17997. The resultant inactivation were confirmed by PCR analysis. The abilities of the PKS gene inactivation strains to produce geldanamycin were compared with the natural geldanamycin- producing strain, S. hygroscopicus 17997. The cos10-inactivated strain exhibited an unchanged ability to produce geldanamycin, but the pg10- inactivated strain can produce twice the yield of the natural strain when grown under the same conditions. We propose that there is a sub-PKS pathway in the geldanamycin-producing strain, S. hygroscopicus 17997.
authors.affiliation: School of Life Sciences and Technology, Henan Cultivating Key Laboratory of Hereditary Disease Targeted Molecular Drugs, Xinxiang Medical University, Xinxiang, China.
authors.forename: Y H
authors.initials: YH
authors.lastname: Li
authors.forename: M
authors.initials: M
authors.lastname: Li
authors.forename: W Q
authors.initials: WQ
authors.lastname: He
authors.forename: Y G
authors.initials: YG
authors.lastname: Wang
authors.forename: R G
authors.initials: RG
authors.lastname: Shao
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D016227:Benzoquinones / Q000378:metabolism*; D003001:Cloning, Molecular; D017124:Conserved Sequence; D005798:Genes, Bacterial; D047029:Lactams, Macrocyclic / Q000378:metabolism*; D008969:Molecular Sequence Data; D005810:Multigene Family; D016254:Mutagenesis, Insertional; D048630:Polyketide Synthases / Q000235:genetics* / Q000378:metabolism*; D013302:Streptomyces / Q000235:genetics* / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D016227:Benzoquinones; D047029:Lactams, Macrocyclic; D048630:Polyketide Synthases; C001277:geldanamycin