title: Fatty acid profiles and adipogenic gene expression of various fat depots in Japanese Black and Holstein steers.
abstract: Objective of the study was to assess the breed effect on fatty acid (FA) composition of different adipose tissues and on mRNA expression of genes involved in adipogenesis and fat metabolism. Japanese Black (JB) and Holstein (HS) steers were kept under equivalent conditions with high energy intake resulting in large differences in intramuscular fat (IMF) accumulation in longissimus muscle (LM). The relative FA composition of muscle, intermuscular fat, visceral fat, and perirenal fat was comparable between JB and HS steers. Circulating fatty acids were also similar in both breeds. Most relevant breed effects were identified in IMF, underlining the uniqueness of this adipose tissue site. JB steers had more monounsaturated FA and less saturated FA. Perilipin 1 and adipose differentiation-related protein (ADFP) mRNA levels were higher in IMF of JB. The results suggest advanced maturity of IMF cells in JB and altered local conditions in muscle influencing IMF accumulation and composition.
authors.affiliation: Laboratory of Nutrition Chemistry, Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School of Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan.
authors.forename: Bungo
authors.initials: B
authors.lastname: Shirouchi
authors.forename: Elke
authors.initials: E
authors.lastname: Albrecht
authors.forename: Gerd
authors.initials: G
authors.lastname: Nuernberg
authors.forename: Steffen
authors.initials: S
authors.lastname: Maak
authors.forename: Samadmanivong
authors.initials: S
authors.lastname: Olavanh
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Nakamura
authors.forename: Masao
authors.initials: M
authors.lastname: Sato
authors.forename: Takafumi
authors.initials: T
authors.lastname: Gotoh
authors.forename: Karin
authors.initials: K
authors.lastname: Nuernberg
mesh_terms: D017667:Adipocytes / Q000378:metabolism; D050156:Adipogenesis / Q000235:genetics*; D000273:Adipose Tissue / Q000378:metabolism*; D000818:Animals; D001947:Breeding; D002417:Cattle; D005227:Fatty Acids / Q000097:blood*; D005229:Fatty Acids, Monounsaturated / Q000097:blood*; D005231:Fatty Acids, Unsaturated / Q000097:blood*; D015870:Gene Expression; D050152:Intra-Abdominal Fat / Q000378:metabolism; D050356:Lipid Metabolism / Q000235:genetics; D008297:Male; D010641:Phenotype; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D015854:Up-Regulation
chemical_list: D005227:Fatty Acids; D005229:Fatty Acids, Monounsaturated; D005231:Fatty Acids, Unsaturated; D012333:RNA, Messenger
keywords: Adipogenesis; Adipose tissue; Fatty acid profile; Holstein; Japanese Black