title: Temporally dimorphic recruitment of dopamine neurons into stress response circuitry in Drosophila.
abstract: Many studies have pointed to vulnerability to stress and stress-related pathologies at different timepoints during an individual's life span. These sensitive windows are usually during periods of neural development, such as embryogenesis, infancy, and adolescence. It is critical to understand how neural circuitry may change as an individual ages in ways that could affect susceptibility to stress. Here we compare two stages in Drosophila melanogaster: sexual immaturity and sexual maturity. We used the genetic resources available in Drosophila to manipulate pre- and postsynaptic dopamine signaling in sexually immature and mature animals that were then assayed for heart rate and locomotor behavior in response to starvation and oxidative stress. Our results show significant differences in the stress response for sexually immature and mature animals for heart rate, periods of high mobility, mean velocity, and several other parameters of locomotor behavior. Our data show that dopamine neurons are differentially recruited into the stress response circuitry for sexually immature and mature individuals. By observing behaviors that have been previously shown in mammalian models to be affected by stress and altered in models of affective disorders, we provide a genetically tractable model for development and maintenance of the stress response circuitry during sexual maturation.
authors.affiliation: Department of Pharmacological and Physiological Science, St. Louis University School of Medicine.
authors.forename: Kathryn J
authors.initials: KJ
authors.lastname: Argue
authors.forename: Wendi S
authors.initials: WS
authors.lastname: Neckameyer
mesh_terms: D000818:Animals; D059290:Dopaminergic Neurons / Q000502:physiology*; D004330:Drosophila; D005260:Female; D008297:Male; D011954:Receptors, Dopamine / Q000378:metabolism; D012727:Sex Characteristics; D012741:Sexual Maturation / Q000502:physiology; D013312:Stress, Physiological
chemical_list: D011954:Receptors, Dopamine
references.citation: Endocrinology. 2006 Apr;147(4):1664-74
references.pmid: 16410296
references.citation: J Neurosci. 1993 May;13(5):1852-61
references.pmid: 8478679
references.citation: Mol Psychiatry. 2009 Oct;14(10):959-67
references.pmid: 19255582
references.citation: Maturitas. 2011 Oct;70(2):141-5
references.pmid: 21802226
references.citation: Mol Psychiatry. 2006 Nov;11(11):992-1002
references.pmid: 16801951
references.citation: J Gen Physiol. 1977 Jun;69(6):705-24
references.pmid: 894240
references.citation: Pharmacol Biochem Behav. 2007 Feb;86(2):220-33
references.pmid: 16901532
references.citation: BMC Neurosci. 2012 Mar 13;13:26
references.pmid: 22413901
references.citation: Nat Neurosci. 1999 Oct;2(10):859-61
references.pmid: 10491602
references.citation: ACS Chem Neurosci. 2011 Dec 21;2(12):723-729
references.pmid: 22308204
references.citation: J Comp Neurol. 2003 Oct 27;465(4):560-78
references.pmid: 12975816
references.citation: J Child Adolesc Psychopharmacol. 2011 Aug;21(4):299-309
references.pmid: 21851188
references.citation: Behav Brain Res. 2008 Dec 1;194(1):86-91
references.pmid: 18640158
references.citation: Dev Psychol. 2009 Jan;45(1):45-55
references.pmid: 19209989
references.citation: Neuroscience. 2008 Oct 28;156(4):841-56
references.pmid: 18790015
references.citation: Brain Res. 2007 Jun 25;1155:196-207
references.pmid: 17493592
references.citation: Nat Rev Neurosci. 2009 Jun;10(6):434-45
references.pmid: 19401723
references.citation: Eur Rev Med Pharmacol Sci. 2012 Feb;16(2):270-5
references.pmid: 22428481
references.citation: Stress. 2005 Jun;8(2):117-31
references.pmid: 16019603
references.citation: Neuroscientist. 2008 Aug;14(4):326-38
references.pmid: 18612087
references.citation: Genesis. 2002 Sep-Oct;34(1-2):1-15
references.pmid: 12324939
references.citation: Brain Res. 1987 Jun 9;413(1):53-9
references.pmid: 2885075