title: Antidiarrheal activity of 19-deoxyicetexone isolated from Salvia ballotiflora Benth in mice and rats.
abstract: The antidiarrheal properties of 19-deoxyicetexone, a diterpenoid isolated from Salvia ballotiflora were evaluated on castor oil-, arachidonic acid (AA)- and prostaglandin (PGE₂)-induced diarrhea in rodent models. The structure of 19-deoxyicetexone was determined by X-ray crystallography, mass spectrometry (EI-MS), as well as ultraviolet (UV-Vis), infrared (FT-IR) and nuclear magnetic resonance (NMR) spectroscopies. This compound significantly and dose-dependently reduced frequency of stooling in castor oil-induced diarrhea, and at dose of 25 mg/kg it also inhibited diarrhea induced with AA, while it had no effect on PGE₂-induced diarrhea. This compound at doses of 25 mg/kg also diminished castor oil-induced enteropooling and intestinal motility, and inhibited the contraction of the rats' ileum induced by carbachol chloride at a concentration of 100 µg/mL. 19-Deoxyicetexone did not present acute toxicity at doses of 625 mg/kg. Its antidiarrheal activity may be due to increased reabsorption of NaCl and water and inhibition of the release of prostaglandins, gastrointestinal motility and fluid accumulation in the intestinal tracts of rats. These findings suggest that 19-deoxyicetexone may be used in the treatment of diarrhea, although more studies must be carried out to confirm this.
authors.affiliation: Departamento de Sistemas Biológicos, Universidad Autónoma Metropolitana, Unidad Xochimilco, Calzada del Hueso 1100, Col. Villa Quietud, Coyoacán 04960, Mexico.
authors.forename: Salud
authors.initials: S
authors.lastname: Pérez-Gutiérrez
authors.forename: Daniel
authors.initials: D
authors.lastname: Zavala-Mendoza
authors.forename: Abigail
authors.initials: A
authors.lastname: Hernández-Munive
authors.forename: Angel
authors.initials: A
authors.lastname: Mendoza-Martínez
authors.forename: Cuauhtemoc
authors.initials: C
authors.lastname: Pérez-González
authors.forename: Ernesto
authors.initials: E
authors.lastname: Sánchez-Mendoza
mesh_terms: D000818:Animals; D000930:Antidiarrheals / Q000008:administration & dosage / Q000737:chemistry / Q000302:isolation & purification; D001892:Camphanes; D018360:Crystallography, X-Ray; D003967:Diarrhea / Q000139:chemically induced / Q000188:drug therapy* / Q000473:pathology; D004224:Diterpenes / Q000008:administration & dosage / Q000737:chemistry / Q000302:isolation & purification*; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000737:chemistry*; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D053723:Panax notoginseng; D051381:Rats; D027561:Salvia miltiorrhiza
chemical_list: C542301:19-deoxyicetexone; D000930:Antidiarrheals; D001892:Camphanes; D004224:Diterpenes; D004365:Drugs, Chinese Herbal; C000604051:danshen dripping pill
references.citation: Am J Physiol. 1989 Jun;256(6 Pt 1):C1224-30
references.pmid: 2472069
references.citation: J Pharm Pharmacol. 1992 Sep;44(9):771-2
references.pmid: 1360533
references.citation: Molecules. 2012 Dec 03;17(12):14288-97
references.pmid: 23208464
references.citation: Am J Dig Dis. 1975 Dec;20(12):1171-7
references.pmid: 1200010
references.citation: Planta Med. 1993 Aug;59(4):333-6
references.pmid: 8372151
references.citation: Fitoterapia. 2008 Jan;79(1):1-5
references.pmid: 17683879
references.citation: J Lipid Res. 2006 Jul;47(7):1416-23
references.pmid: 16585783
references.citation: Jpn J Pharmacol. 2000 Feb;82(2):168-70
references.pmid: 10877537
references.citation: Bull World Health Organ. 1982;60(4):605-13
references.pmid: 6982783
references.citation: Naunyn Schmiedebergs Arch Pharmacol. 1996 May;353(6):680-4
references.pmid: 8738301
references.citation: J Smooth Muscle Res. 2010;46(2):107-17
references.pmid: 20551591
references.citation: J Pharm Pharmacol. 1978 Jan;30(1):41-5
references.pmid: 22723
references.citation: J Pharmacol Exp Ther. 1997 Jul;282(1):403-9
references.pmid: 9223581
references.citation: Prostaglandins. 1976 May;11(5):809-28
references.pmid: 935512
references.citation: J Pharm Pharmacol. 1983 Mar;35(3):152-6
references.pmid: 6132971
references.citation: J Pharm Pharmacol. 1988 Jan;40(1):79-82
references.pmid: 2896789
references.citation: Br J Pharmacol. 1994 Dec;113(4):1127-30
references.pmid: 7889264
references.citation: Inflamm Bowel Dis. 2006 Mar;12(3):153-65
references.pmid: 16534415