title: Cardiorespiratory responses to graded hypoxia in the neotropical fish matrinxã (Brycon amazonicus) and traíra (Hoplias malabaricus) after waterborne or trophic exposure to inorganic mercury.
abstract: The growing Hg input in aquatic environments results in high accumulation of mercury in fish tissue and their consumers, which poses a serious risk to humans and ecosystems. The aim of this study was to evaluate the effects of the inorganic mercury exposure on cardiorespiratory responses in two species of neotropical fish ecologically distinct, matrinxã (Brycon amazonicus) and traíra (Hoplias malabaricus). Matrinxãs were exposed to a nominal and sublethal concentration of 0.15 mgL(-1) of HgCl2 for 96 h. Traíras were exposed to trophic doses (each 4 days, during 30 days) of inorganic Hg (0.45 mg as total Hg) using juvenile B. amazonicus as prey vehicle. The metabolic rate (VO2), critical oxygen tensions (PcO2), gill ventilation (VG), tidal volume (VT), respiratory frequency (fR), O2 extraction from the ventilatory current (EO2), and heart rate (fH) were measured under normoxia (140 mm Hg) and graded hypoxia (120, 100, 80, 60, 40, 20, and 10 mm Hg). Regarding matrinxã specifically, the critical point highlighted was tachypnea. In traíras, bradypnea, decreased metabolic rate and O2 extraction, severe bradycardia, and elevated tidal volume were observed in normoxia. Both acute and sub-chronic exposures increased the critical tension of O2 values in more than 100%. In addition, Hg exposures modulated hypoxia-induced responses resulting in impairment of cardio-respiratory system of both species. Thus, mercury, via food or water, decreases the plasticity of the cardiorespiratory responses reducing the survival chances of B. amazonicus and H. malabaricus under hypoxic conditions frequently observed in theirs wild habitats.
authors.affiliation: Department of Physiological Sciences, Federal University of São Carlos, São Carlos, São Paulo, Brazil.
authors.forename: Diana Amaral
authors.initials: DA
authors.lastname: Monteiro
authors.forename: Juliana Montovani
authors.initials: JM
authors.lastname: Thomaz
authors.forename: Francisco Tadeu
authors.initials: FT
authors.lastname: Rantin
authors.forename: Ana Lúcia
authors.initials: AL
authors.lastname: Kalinin
mesh_terms: D000818:Animals; D001481:Basal Metabolism / Q000187:drug effects; D060126:Characidae / Q000502:physiology*; D005880:Gills / Q000187:drug effects; D006321:Heart / Q000187:drug effects*; D006339:Heart Rate / Q000187:drug effects; D000860:Hypoxia / Q000503:physiopathology; D008628:Mercury / Q000633:toxicity*; D010101:Oxygen Consumption / Q000187:drug effects; D056152:Respiratory Rate / Q000187:drug effects; D012137:Respiratory System / Q000187:drug effects*
chemical_list: D008628:Mercury
keywords: Heart rate; Inorganic mercury; Metabolic rate; Oxygen extraction; Respiratory responses