title: Plant regeneration methods for rapid generation of a large scale Ds transposant population in rice.
abstract: To mutagenize rice genomes, a two-element system is utilized. This system comprises an immobile Ac element driven by the CaMV 35S promoter, and a gene trap Ds carrying a partial intron with alternative splice acceptors fused to the GUS coding region. Rapid, large-scale generation of a Ds transposant population was achieved using a plant regeneration procedure involving the tissue culture of seed-derived calli carrying Ac and Ds elements. During tissue cultures, Ds mobility accompanies changes in methylation patterns of a terminal region of Ds, where over 70% of plants contained independent Ds insertions. In the transposon population, around 12% of plants expressed GUS at the early seedling stage. A flanking-sequence-tag (FST) database has been established by cloning over 19,968 Ds insertion sites and the Ds map shows relatively uniform distribution across the rice chromosomes.
authors.affiliation: Division of Applied Life Science (BK21 Program), Plant Molecular Biology and Biotechnology Research Center (PMBBRC), Gyeongsang National University, Jinju, Korea.
authors.forename: Yuan Hu
authors.initials: YH
authors.lastname: Xuan
authors.forename: Jin
authors.initials: J
authors.lastname: Huang
authors.forename: Gihwan
authors.initials: G
authors.lastname: Yi
authors.forename: Dong-Soo
authors.initials: DS
authors.lastname: Park
authors.forename: Soo Kwon
authors.initials: SK
authors.lastname: Park
authors.forename: Moo Young
authors.initials: MY
authors.lastname: Eun
authors.forename: Doh Won
authors.initials: DW
authors.lastname: Yun
authors.forename: Gang-Seob
authors.initials: GS
authors.lastname: Lee
authors.forename: Tae Ho
authors.initials: TH
authors.lastname: Kim
authors.forename: Chang-deok
authors.initials: CD
authors.lastname: Han
mesh_terms: D001483:Base Sequence; D004251:DNA Transposable Elements / Q000235:genetics*; D018744:DNA, Plant / Q000235:genetics; D005818:Genetic Engineering / Q000379:methods*; D023281:Genomics; D016296:Mutagenesis; D012275:Oryza / Q000235:genetics* / Q000254:growth & development*; D030821:Plants, Genetically Modified; D012038:Regeneration; D013997:Time Factors; D046509:Tissue Culture Techniques
chemical_list: D004251:DNA Transposable Elements; D018744:DNA, Plant