Production of 2,3-butanediol by engineered Saccharomyces cerevisiae.
In order to produce 2,3-butanediol (2,3-BD) with a high titer, it is necessary to engineer Saccharomyces cerevisiae by deleting the competing pathway and overexpressing the 2,3-BD biosynthetic pathway. A pyruvate decarboxylase (Pdc)-deficient mutant was constructed and evolved for rapid glucose consumption without ethanol production. Genome re-sequencing of the evolved strain (SOS4) revealed a point mutation (A81P) in MTH1 coding for a transcriptional regulator involved in glucose sensing, unlike the previously reported Pdc-deficient mutant which had internal deletion in MTH1. When alsS and alsD genes from Bacillus subtilis, and endogenous BDH1 gene were overexpressed in SOS4, the resulting strain (BD4) not only produced 2,3-BD efficiently, but also consumed glucose faster than the parental strain. In fed-batch fermentation with optimum aeration, 2,3-BD concentration increased up to 96.2 g/L. These results suggest that S. cerevisiae might be a promising host for producing 2,3-BD for industrial applications.
Department of Agricultural Biotechnology, Seoul National University, Seoul 151-921, South Korea; Center for Food and Bioconvergence, Seoul National University, Seoul 151-921, South Korea.
Soo-Jung
SJ
Kim
Department of Food Science and Human Nutrition, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA; Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.
Seung-Oh
SO
Seo
Department of Food Science and Human Nutrition, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA; Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA. Electronic address: ysjin@illinois.edu.
Yong-Su
YS
Jin
Department of Agricultural Biotechnology, Seoul National University, Seoul 151-921, South Korea; Center for Food and Bioconvergence, Seoul National University, Seoul 151-921, South Korea. Electronic address: jhseo94@snu.ac.kr.
Jin-Ho
JH
Seo
D000095:Acetolactate Synthase / Q000235:genetics; D001412:Bacillus subtilis / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D002072:Butylene Glycols / Q000737:chemistry*; D002262:Carboxy-Lyases / Q000235:genetics; D005285:Fermentation; D017353:Gene Deletion; D005798:Genes, Bacterial; D005818:Genetic Engineering; D005838:Genotype; D005947:Glucose / Q000378:metabolism; D007218:Industrial Microbiology; D008969:Molecular Sequence Data; D009154:Mutation; D010957:Plasmids / Q000378:metabolism; D017354:Point Mutation; D011767:Pyruvate Decarboxylase / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism*; D017422:Sequence Analysis, DNA; D013997:Time Factors
D002072:Butylene Glycols; C026978:2,3-butylene glycol; D000095:Acetolactate Synthase; D002262:Carboxy-Lyases; D011767:Pyruvate Decarboxylase; C023746:acetolactate decarboxylase; D005947:Glucose
2,3-Butanediol (2,3-BD); MTH1; Pyruvate decarboxylase-deficient mutant; Saccharomyces cerevisiae