Association of polymorphism of ser311cys paraoxonase-2 gene with type 2 diabetes mellitus in iran.
BACKGROUND
It is believed that paraoxonase-2 gene polymorphism is associated with type 2 diabetes. This study is aimed to investigate the association between paraoxonase-2 gene polymorphism and type 2 diabetes in an Iranian population.


METHODS
This study was performed on 200 individuals including 100 diabetics and 100 healthy subjects. Genomic DNA was extracted from peripheral blood leukocytes, and PCR-RFLP was carried out. Palindromic sequence in PON2 gene was recognized by Dde1 restriction endonuclease. In order to visualize restriction products, electrophoresis was carried out using polyacrylamide gel (8%) and ethidium bromide staining.


RESULTS
The expected PCR product of 331 bp was obtained. Digestion of this product with DdeI showed four Ser homozygotes, three Cys homozygotes, and five Ser311 Cys heterozygotes. The gene frequency of Cys (C) in diabetic subjects was significantly higher than in healthy subjects.


CONCLUSIONS
This study suggests that an association exists between Ser311 Cys polymorphism and type 2 diabetes mellitus.
Neurosciences Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
Sasan
S
Andalib
Golnaz
G
Vaseghi
Azadeh
A
Motavallian
Hamid Mirmohamad
HM
Sadeghi
Azadeh
A
Eshraghi
Masoud
M
Amini
Amir-Reza
AR
Majlesi
Iranian population; paraxonase-2 gene; polymorphism; type 2 diabetes