High density microarrays on Blu-ray discs for massive screening.
The potential and the capabilities of Blu-ray technology (discs and drives) for massive screening applications are presented. High density microarrays are fabricated onto a Blu-ray disc and applied for the determination of microcystin residues and pathogenic microorganisms. Specific probes were physisorbed onto the BD surface and the biorecognition event was displayed using labeled secondary antibody solution and subsequent signal amplification. The attenuation of the reflected light caused by the reaction product is detected by the Blu-ray drive and inversely correlated with analyte concentration. BDs preserve the optical properties according to Blu-ray specifications, ensuring maximum accuracy and sensitivity of the drive during disc scanning. Detection limits of 0.4 μg/L for microcystin LR and 10(0) and 10(1) cfu/mL for Salmonella typhimurium and Cronobacter sakazakii respectively, were achieved, improving considerably the DVD performances and reaching similar sensitivity as real-time quantitative PCR. Blu-ray technology adapted to the analysis of high density arrays highlights the enormous capabilities (high sensitivity, speed-scanning, optical resolution, portability) for point-of-care settings, diagnostics, and high-throughput screening applications.
Centro de Reconocimiento Molecular y Desarrollo Tecnológico, Departamento de Química, Universitat Politècnica de València, Camino de Vera s/n, 46071 Valencia, Spain.
Tania
T
Arnandis-Chover
Sergi
S
Morais
Miguel Ángel
MÁ
González-Martínez
Rosa
R
Puchades
Ángel
Á
Maquieira
D015374:Biosensing Techniques / Q000295:instrumentation*; D044083:Cronobacter sakazakii / Q000235:genetics / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D004867:Equipment Design; D057230:Limit of Detection; D008387:Marine Toxins; D046228:Microarray Analysis / Q000295:instrumentation*; D052998:Microcystins / Q000032:analysis*; D009693:Nucleic Acid Hybridization; D012486:Salmonella typhimurium / Q000235:genetics / Q000302:isolation & purification
D004269:DNA, Bacterial; D008387:Marine Toxins; D052998:Microcystins; C057862:cyanoginosin LR
Blu-ray; High density arrays; Massive screening; Multiplex analysis; Point-of-care