The inhibitory effect of naringenin on atopic dermatitis induced by DNFB in NC/Nga mice.
OBJECTIVE
Atopic dermatitis (AD) is a chronic and relapsing inflammatory dermatitis characterized by pruritic and eczematous skin lesions. Here, we investigated the therapeutic effect of the fruit flavonoid naringenin on DNFB induced atopic dermatitis mice model.


METHODS
AD-like skin lesion was induced by repetitive skin contact with DNFB in NC/Nga mice and the effects of the fruit flavonoid naringenin were evaluated on the basis of histopathological findings of skin, ear swelling and cytokine production of CD4(+)T cells.


RESULTS
Intraperitoneal injection of naringenin for one week after DNFB challenge significantly lowered ear swelling and improved back skin lesions. In addition, naringenin significantly suppressed production of interferon-gamma (IFN-γ) by activated CD4(+) T cells and serum IgE level. Furthermore, naringenin reduced DNFB-induced infiltration of eosinophils, mast cells, CD4(+) T cells, and CD8(+) T cells in skin lesions.


CONCLUSIONS
Naringenin may suppress the development of AD-like skin lesions in DNFB-treated NC/Nga mice by reducing IFN-γ production of activated CD4(+) T cells, serum IgE levels and infiltration of immune cells to skin lesion.
Department of Microbiology, School of Medicine, Kyung Hee University, Seoul, Republic of Korea.
Tae-Ho
TH
Kim
Gun-Dong
GD
Kim
Hyun-Jong
HJ
Ahn
Jeong-Je
JJ
Cho
Yong Seek
YS
Park
Cheung-Seog
CS
Park
D000818:Animals; D000897:Anti-Ulcer Agents / Q000627:therapeutic use*; D015496:CD4-Positive T-Lymphocytes / Q000378:metabolism; D016207:Cytokines / Q000096:biosynthesis; D003876:Dermatitis, Atopic / Q000097:blood / Q000139:chemically induced* / Q000188:drug therapy* / Q000473:pathology; D004139:Dinitrofluorobenzene / Q000009:adverse effects*; D004423:Ear / Q000473:pathology; D004487:Edema / Q000188:drug therapy; D044950:Flavanones / Q000627:therapeutic use*; D007073:Immunoglobulin E / Q000097:blood; D007249:Inflammation / Q000188:drug therapy*; D007371:Interferon-gamma / Q000096:biosynthesis; D008297:Male; D051379:Mice; D012867:Skin / Q000187:drug effects / Q000473:pathology
D000897:Anti-Ulcer Agents; D016207:Cytokines; D044950:Flavanones; D007073:Immunoglobulin E; D007371:Interferon-gamma; D004139:Dinitrofluorobenzene; C005273:naringenin
2,4-dinitrofluorobenzene; Atopic dermatitis; NC; Naringenin; Nga mice; Skin barrier