Beta amyloid peptide (25-35) leading to inflammation through Toll-like receptors and the anti-inflammatory effect of genistein in BV-2 cells.
Genistein, the main soy isoflavone component, has received much attention for its potential multifunction. Here, we reported that in BV-2 cells, genistein significantly inhibited beta amyloid peptides 25-35 (Aβ25-35)-induced inflammatory response. The results indicated that Aβ25-35-stimulated BV-2 cells upregulated Toll-like receptors 2 and 4, Myd88, and IKK gene expression with the increasing expression of IL-6 and decreasing expression of TGF-β and IL-10. Further, inhibiting TLR4 expression with small interfering RNA prevented the inflammatory response induced by Aβ25-35, indicating the key role of TLRs in Aβ-mediated inflammation. Genistein pre-treated BV-2 cells showed less inflammatory response when exposed to Aβ25-35. These results suggested that Aβ induced BV-2 cells inflammation though TLRs and genistein has an anti-inflammatory effect in vitro.
School of Public Health, Capital Medical University, No.10 Xitoutiao, You An Men, Beijing, People's Republic of China, yuhlzjl@ccmu.edu.cn.
Huan-Ling
HL
Yu
Xiao-Ying
XY
Li
Xin
X
Zhou
Lin-Hong
LH
Yuan
Wei-Wei
WW
Ma
Yuan-Di
YD
Xi
Xia
X
Zhao
Jian
J
Wu
Rong
R
Xiao
D016229:Amyloid beta-Peptides / Q000633:toxicity*; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D002460:Cell Line; D019833:Genistein / Q000494:pharmacology*; D051550:I-kappa B Kinase / Q000235:genetics / Q000378:metabolism; D007249:Inflammation / Q000139:chemically induced / Q000378:metabolism; D016753:Interleukin-10 / Q000235:genetics / Q000378:metabolism; D015850:Interleukin-6 / Q000235:genetics / Q000378:metabolism; D051379:Mice; D053594:Myeloid Differentiation Factor 88 / Q000235:genetics / Q000378:metabolism; D010446:Peptide Fragments / Q000633:toxicity*; D051197:Toll-Like Receptor 4 / Q000235:genetics / Q000378:metabolism*; D016212:Transforming Growth Factor beta / Q000235:genetics / Q000378:metabolism
D016229:Amyloid beta-Peptides; D000894:Anti-Inflammatory Agents, Non-Steroidal; C556062:IL10 protein, mouse; D015850:Interleukin-6; C507780:Myd88 protein, mouse; D053594:Myeloid Differentiation Factor 88; D010446:Peptide Fragments; C493487:Tlr4 protein, mouse; D051197:Toll-Like Receptor 4; D016212:Transforming Growth Factor beta; C074636:amyloid beta-protein (25-35); C577686:interleukin-6, mouse; D016753:Interleukin-10; D019833:Genistein; D051550:I-kappa B Kinase