Combined two-photon microscopy and angiographic optical coherence tomography.
A combined two-photon microscopy (TPM) and angiographic optical coherence tomography (OCT) is developed, which can provide molecular, cellular, structural, and vascular information of tissue specimens in vivo. This combined system is implemented by adding an OCT vasculature visualization method to the previous combined TPM and OCT, and then is applied to in vivo tissue imaging. Two animal models, a mouse brain cranial window model and a mouse ear cancer model, are used. Both molecular, cellular information at local regions of tissues, and structural, vascular information at relatively larger regions are visualized in the same sections. In vivo tissue microenvironments are better elucidated by the combined TPM and angiographic OCT.
Bumju
B
Kim
Tae Jun
TJ
Wang
Qingyun
Q
Li
Jutaek
J
Nam
Sekyu
S
Hwang
Euiheon
E
Chung
Sungjee
S
Kim
Ki Hean
KH
Kim
D000792:Angiography / Q000379:methods*; D000818:Animals; D045744:Cell Line, Tumor; D004428:Ear Neoplasms / Q000098:blood supply* / Q000150:complications / Q000473:pathology*; D007090:Image Interpretation, Computer-Assisted / Q000379:methods; D051379:Mice; D036641:Microscopy, Fluorescence, Multiphoton / Q000379:methods*; D064847:Multimodal Imaging / Q000379:methods*; D009389:Neovascularization, Pathologic / Q000150:complications / Q000473:pathology*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013382:Subtraction Technique; D041623:Tomography, Optical Coherence / Q000379:methods*