Coupling of random amplified polymorphic DNA profiles analysis and high resolution capillary electrophoresis system for the assessment of chemical genotoxicity.
Cadmium (Cd) can be toxic to terrestrial snails, but few data are available about its genotoxic effects on early life stages (ELS). The aim of this study was to investigate the genotoxic potential of Cd in embryos of Helix aspersa using a new approach that couples Random Amplified Polymorphic DNA (RAPD) and a high-resolution capillary electrophoresis system (HRS). Clutches of H. aspersa were exposed to Cd solutions (2, 4, and 6 mg/L) from the beginning of their embryonic development. In addition to a dose-dependent effect of Cd on hatching rate, DNA fragmentation was observed in embryos that were exposed to 6 mg Cd/L. The analysis of RAPD products with HRS showed differences between the profiles of exposed and nonexposed embryos, starting at 2 mg Cd/L. In comparison to the profiles of the control samples, all profiles from the exposed snails exhibited an additional 270 bp DNA fragment and lacked a 450 bp DNA fragment. These profile modifications are related to the genotoxic effect of Cd on the ELS of H. aspersa . Our study demonstrates the efficacy of coupling RAPD and HRS for a rapid and efficient screening of the effects of chemicals on DNA.
Chrono-Environment, UMR 6249, University of Franche-Comté/CNRS , Place Leclerc, 25000 Besançon, France.
Pierre-Emmanuel
PE
Baurand
Annette
A
de Vaufleury
Renaud
R
Scheifler
Nicolas
N
Capelli
D000818:Animals; D002104:Cadmium / Q000633:toxicity*; D019075:Electrophoresis, Capillary; D004625:Embryo, Nonmammalian; D006372:Helix, Snails; D009152:Mutagenicity Tests; D019105:Random Amplified Polymorphic DNA Technique; D018675:Toxicity Tests
D002104:Cadmium