Co-administration of flagellin augments immune responses to inactivated foot-and-mouth disease virus (FMDV) antigen.
Foot-and-mouth disease virus (FMDV) is one of the most contagious animal virus known that affects livestock health and production. This study aimed to investigate the effect of flagellin, a toll-like receptor 5 agonist, on the immune responses to inactivated FMDV antigen in guinea pig model. Our results showed that the co-administration of flagellin with FMDV antigen through intradermal route induces earlier and higher anti-FMDV neutralizing antibody responses as compared to FMDV antigen alone. Both IgG1 and IgG2 antibody-isotype responses were enhanced, but the IgG1/IgG2 ratios were relatively low, indicative of TH1 type of immune activation. On live viral challenge, flagellin+FMDV immunized guinea pigs showed 70% (7 out of 10) protection rate as compared to 40% (4 out of 10) in FMDV alone immunized guinea pigs. The results demonstrate that the co-administration of flagellin augments immune responses (preferably TH1 type) and protective efficacy against FMDV in guinea pigs.
FMD Research Centre, Indian Veterinary Research Institute, Bangalore 560024, India.
Irshad A
IA
Hajam
Pervaiz A
PA
Dar
Shanmugam
S
Chandrasekar
Raj Kishore
RK
Nanda
Subodh
S
Kishore
Veerakyathappa
V
Bhanuprakash
Kondabattula
K
Ganesh
D000818:Animals; D057134:Antibodies, Neutralizing / Q000276:immunology; D000956:Antigens, Viral / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005260:Female; D005408:Flagellin / Q000276:immunology / Q000494:pharmacology*; D029544:Foot-and-Mouth Disease Virus / Q000276:immunology*; D006168:Guinea Pigs; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D008297:Male; D009500:Neutralization Tests / Q000662:veterinary; D011994:Recombinant Proteins / Q000276:immunology
D057134:Antibodies, Neutralizing; D000956:Antigens, Viral; D000949:Histocompatibility Antigens Class II; D011994:Recombinant Proteins; D005408:Flagellin
Flagellin; Foot-and-mouth disease virus; Guinea pig; Immune response; Molecular adjuvant