Microwave-assisted solid-phase peptide synthesis using the biotage Syro Wave™.
Fast and precise heating by microwave irradiation during solid-phase peptide synthesis (SPPS) can reduce reaction times as well as provide better purities and greater yields for the synthesis of difficult peptides. Microwave- assisted SPPS has proven to be a useful and reliable tool for the synthesis of peptides as well as small proteins. It is particularly well suited for sequences with a high propensity to form β-sheet-type structures and for sterically difficult couplings. In this protocol, conditions and detailed procedures are described for performing microwave-assisted SPPS using the Syro Wave™. Here we describe the synthesis of two difficult peptide sequences: the first is derived from the C-terminus of the MuLV CTL epitope, the second is a de novo designed peptide with a C-terminal alkyne.
Department of Chemistry, Faculty of Sciences, University of Copenhagen, Frederiksberg, Denmark.
Leila
L
Malik
Knud J
KJ
Jensen
D008872:Microwaves; D010455:Peptides / Q000138:chemical synthesis*; D060327:Solid-Phase Synthesis Techniques / Q000295:instrumentation* / Q000379:methods*
D010455:Peptides