Characterization of a Yariv precipitated arabinogalactan-protein from fruits of rye (Secale cereale L.).
An arabinogalactan-protein (AGP) with a molecular mass of 110 kDa was isolated from whole grain of rye (Secale cereale L.) by double precipitation with (beta-D-Glc)3-Yariv-phenylglycoside (3GlcY) and its structure was analyzed. The AGP consists of a hydroxyproline-rich protein backbone of about 7 % and an arabinogalactan moiety of about 93%. By alkaline hydrolysis, hydroxyproline was identified as the main amino acid responsible for the binding between the protein and the carbohydrate subunits via an O-glycosidic linkage. The arabinogalactan moieties are highly branched consisting of 1,3-linked Galp residues, some of them linked in position 6 to 1,6-Galp side chains, terminating in Araf residues. With regard to its structure, the rye AGP is comparable to other cereal AGPs like those from oat or wheat grain.
Pharmaceutical Institute, Department of Pharmaceutical Biology, Christian-Albrechts-University of Kiel, Germany. mgoellner@pharmazie.uni-kiel.de
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Blaschek
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Classen
D000596:Amino Acids / Q000032:analysis; D002241:Carbohydrates / Q000737:chemistry; D002850:Chromatography, Gel; D002523:Edible Grain / Q000737:chemistry; D005504:Food Analysis; D005685:Galactans / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D005960:Glucosides / Q000737:chemistry*; D006868:Hydrolysis; D006909:Hydroxyproline / Q000032:analysis; D005779:Immunodiffusion; D008745:Methylation; D008970:Molecular Weight; D009005:Monosaccharides / Q000032:analysis; D010696:Phloroglucinol / Q000031:analogs & derivatives* / Q000737:chemistry; D012434:Secale / Q000737:chemistry*
D000596:Amino Acids; D002241:Carbohydrates; D005685:Galactans; D005960:Glucosides; D009005:Monosaccharides; C104977:Yariv reagent; D010696:Phloroglucinol; D006909:Hydroxyproline; C005653:arabinogalactan