Inhibition of stearoyl-CoA desaturase 1 reduces lipogenesis in primary bovine adipocytes.
The objectives were to determine the effect of stearoyl-CoA desaturase (SCD1) inhibition on adipocyte proliferation, differentiation and cellular lipid metabolism in bovine primary adipocytes. Inhibition of SCD1 activity by sterculic acid (SA) or conjugated linoleic acid, trans-10 cis-12 isomer, (t10, c12-CLA) did not alter adipocyte cellular proliferation, viability or differentiation. In 1,2-[(13)C]-acetate supplemented cells, the mass isotopomer distribution analysis showed that the fractional synthesis rate of [(13)C]-16:0 was reduced (P < 0.01) in SA and t10, c12-CLA treatments compared to control. Of the lipogenic genes, t10, c12-CLA treatment decreased (P < 0.05) the expression of SCD1, acetyl-CoA carboxylase (ACC), fatty acid synthase; whereas SA supplementation decreased (P < 0.05) the expression of ACC. Both SA and t10, c12-CLA increased (P < 0.05) the expression of hormone-sensitive lipase and carnitine palmitoyl transferase involved in lipolysis and oxidation. Inhibition of SCD1 in bovine adipocytes decreases de novo fatty acid synthesis by down-regulating genes involved in lipogenesis and up-regulating genes involved in lipolysis and oxidation.
Clemson University, Clemson, SC, USA.
A K G
AK
Kadegowda
T A
TA
Burns
S L
SL
Pratt
S K
SK
Duckett
D017667:Adipocytes / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology; D000818:Animals; D002417:Cattle; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003521:Cyclopropanes / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005227:Fatty Acids / Q000096:biosynthesis; D005229:Fatty Acids, Monounsaturated / Q000494:pharmacology; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects*; D044243:Linoleic Acids, Conjugated / Q000494:pharmacology; D050356:Lipid Metabolism / Q000187:drug effects; D008055:Lipids; D050155:Lipogenesis / Q000187:drug effects*; D013230:Stearoyl-CoA Desaturase / Q000037:antagonists & inhibitors*; D002787:Sterol Esterase / Q000235:genetics; D014166:Transferases / Q000235:genetics
D003521:Cyclopropanes; D005227:Fatty Acids; D005229:Fatty Acids, Monounsaturated; D044243:Linoleic Acids, Conjugated; D008055:Lipids; C496197:trans-10,cis-12-conjugated linoleic acid; C523776:SCD1 protein, human; D013230:Stearoyl-CoA Desaturase; D014166:Transferases; D002787:Sterol Esterase; C003345:sterculic acid