Regulation of long-range planar cell polarity by Fat-Dachsous signaling.
Fat (Ft) and Dachsous (Ds) are large cadherins that bind each other and have conserved roles in regulating planar cell polarity (PCP). We quantitatively analyzed Ft-Ds pathway mutant clones for their effects on ommatidial polarity in the Drosophila eye. Our findings suggest that the Ft-Ds pathway regulates PCP propagation independently of asymmetric cellular accumulation of Ft or Ds. We find that the Ft effector Atrophin has a position-specific role in regulating polarity in the eye, and that asymmetric accumulation of the atypical myosin Dachs is not essential for production and propagation of a long-range PCP signal. Our observations suggest that Ft and Ds interact to modulate a secondary signal that regulates long-range polarity, that signaling by the Ds intracellular domain is dependent on Ft, and that ommatidial fate specification is genetically separable from long-range signaling.
Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, ON M5G 1X5, Canada.
Praveer
P
Sharma
Helen
H
McNeill
D000818:Animals; D015820:Cadherins / Q000737:chemistry / Q000378:metabolism*; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D016764:Cell Polarity; D002999:Clone Cells; D029721:Drosophila Proteins / Q000737:chemistry / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000166:cytology* / Q000378:metabolism*; D005123:Eye / Q000166:cytology / Q000378:metabolism; D008954:Models, Biological; D009154:Mutation / Q000235:genetics; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D021381:Protein Transport
D015820:Cadherins; D015815:Cell Adhesion Molecules; D029721:Drosophila Proteins; C094449:ds protein, Drosophila; C472307:ft protein, Drosophila
Atrophin; Dachsous; Drosophila; Fat; PCP; Polarity