[Simultaneous determination of five constituents in eight Qingyedan species derived from Swertia plants by HPLC].
OBJECTIVE
To develop an HPLC method for simultaneous determination of swertiamarin, gentiopicroside, sweroside, mangiferin, erythrocentaurin, and to detect these five constituents in eight Qingyedans derived from Swertia mileensis, S. cincta, S. patens, S. punicea, S. delavayi, S. nervosa, S. macrosperma and S. yunnanensis.


METHODS
The separation was carried out on a Thermo BDS Hypersil C18 (4. 6 mm x 250 mm, 5 microm) column eluted with mobile phase of water containing 0. 1% phosphoric acid and methanol (B) in gradient program (0-10 min, 18%-20% B; 10-30 min, 20%-35% B; 30-35 min, 35%-60% B). The column temperature was 32 degrees C , and the detection wavelength was set at 250, 260, 225 nm. The flow rate was 0. 7 mL . min-1 from 0 to 30 min, and be increased to 1. 0 mL . min-1 in 35 min.


RESULTS
The five compounds were well separated. The linear response ranges of swertiamarin, gentiopicroside, sweroside, mangiferin, erythrocentaurin were 0. 072-13. 39, 0. 1204. 518, 0. 060-5. 050, 0. 025-1. 518, and 0. 031-0. 210 microg, respectively. The mean recoveries of five compounds were 97.03% -102. 7% (RSD 1. 8% -6.2% ). There are swertiamarin, gentiopicroside and sweroside in most samples, and mangiferin in half samples. But erythrocentaurin was only detected in a few samples. The contents of five compounds were different in different samples. The contents of swertiamarin in S. mileensis, S. patens, S. yunnanensis and S. delavayi are up to 34. 47-118.05 mg . g-1, the contents of gentiopicroside are up to 25. 91 mg . g-1 in S. cincta. In S. puncea all contents of swertiamarin, gentiopicroside, sweroside and mangiferin are higher, especially the content of sweroside. There are Xiao-Qingyedans and Da-Qingyedans called in markets, and they can be identified by the contents of swertiamarin, gentiopicroside and sweroside. S. punicea can be identified by the content of sweroside, and the ratio gentiopicroside/total content can be used for identification of S. cincta from other seven Qingyedan species.


CONCLUSIONS
The method was certified to be accurate and reliable and can be used for identification and quality evaluation of traditional Chinese medicine Qingyedan derived from Swertia species.
State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunmning Institute of Botany, Chinese Academy of Sciences, Kunming 650204, China. liyaoli123456@163.com
Yao-Li
YL
Li
Ming-Ying
MY
Shang
Chang-An
CA
Geng
Xue-Mei
XM
Zhang
Ji-Jun
JJ
Chen
Shao-Qing
SQ
Cai
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D057888:Iridoid Glucosides / Q000032:analysis; D011753:Pyrones / Q000032:analysis; D029967:Swertia / Q000737:chemistry*
D057888:Iridoid Glucosides; D011753:Pyrones; C012997:gentiopicroside; C013270:swertiamarin; C049412:sweroside