Discrimination of malignant neutrophils of chronic myelogenous leukemia from normal neutrophils by support vector machine.
Malignant neutrophils of chronic myelogenous leukemia (CML) have similar antigen expression patterns compared to their normal counterparts, thus making the cells difficult to distinguish by clinical flow cytometry. In this study, we applied the support vector machine method to build a malignant neutrophil prediction model based on nine CML patients and nine healthy donors. This approach effectively differentiated between malignant and normal neutrophils with high specificity and sensitivity (≤95.80% and ≤95.30%, respectively). This approach may broaden the application of flow cytometry for differentiation between CML and normal neutrophils and become an important diagnostic tool in CML.
Institute of Hematology, The First Affiliated Hospital, College of Medicine, Zhejiang University, 79# Qingchun Road, Hangzhou 310003, PR China.
Wanmao
W
Ni
Xiangmin
X
Tong
Wenbin
W
Qian
Jie
J
Jin
Hongchan
H
Zhao
D000328:Adult; D000368:Aged; D000951:Antigens, Neoplasm / Q000096:biosynthesis*; D005260:Female; D005434:Flow Cytometry / Q000379:methods*; D015973:Gene Expression Regulation, Leukemic; D006801:Humans; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000097:blood / Q000175:diagnosis / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009504:Neutrophils / Q000378:metabolism / Q000473:pathology; D060388:Support Vector Machine
D000951:Antigens, Neoplasm
ABL; Abelson murine leukemia; BCR; CML; FCS; Flow cytometry; Immunophenotyping; LIBLINEAR; Mature neutrophils; PCR; ROC; SVM; Support vector machine; breakpoint cluster region; chronic myelogenous leukemia; flow cytometry standard; polymerase chain reaction; receiver operating characteristic; support vector machine