[Accurate identification of Psammosilene tunicoides and its confused species by systematic identification method].
OBJECTIVE
To develop an effective identification method for accurately discriminating Psammosilene tunicoides and its confused species by the combined method of microscopic identification and molecular identification, so-called systematic identification of Chinese materia medica (SICMM).


METHODS
P. tunicoides and its confused species were accurately discriminated by SICMM method, which was established by comprehensively use of microscopic identification and DNA identification method. The DNA identification included the following analysis: the BLAST alignment, specific bases and N-J phylogenetic tree analysis.


RESULTS
The cluster crystals were not observed in P. tunicoides, but great deals of them were found in Silene viscidula. Further more, big differences of ITS sequence were observed and analyzed between P. tunicoides and its confused specie of S. viscidula.


CONCLUSIONS
The system method is a scientific and accurate method for the identification of P. tunicoides and its counterfeit species.
School of Chinese Materia Medica, Beijing University of Traditional Chinese Medicine, Beijing 100102, China.
Cai-Li
CL
Liao
Chun-Sheng
CS
Liu
Yuan-Yuan
YY
Zhang
Hao-Zhong
HZ
Wu
Xue-Yong
XY
Wang
Xiao-Li
XL
Cheng
Guo-Jie
GJ
Xu
D001483:Base Sequence; D029748:Caryophyllaceae / Q000737:chemistry / Q000145:classification* / Q000166:cytology / Q000235:genetics*; D021901:DNA, Intergenic; D010641:Phenotype; D010802:Phylogeny; D016415:Sequence Alignment
D021901:DNA, Intergenic