A unique family of rigid analogues of the GFP chromophore with tunable two-photon action cross-sections for biological imaging.
Conformationally restricted analogues of the GFP chromophore have been synthesized. The spectroscopic properties of GCTPOC, a unique family with tunable two-photon action cross-sections, were investigated. GCTPOC could be used as a robust two-photon platform for development of a two-photon fluorescent thiol sensor, which could stain endogenous thiols both in vitro and in vivo.
State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, Hunan 410082 (China).
Lin
L
Yuan
Weiying
W
Lin
Hua
H
Chen
Sasa
S
Zhu
Longwei
L
He
D005456:Fluorescent Dyes / Q000737:chemistry*; D049452:Green Fluorescent Proteins / Q000737:chemistry*; D008968:Molecular Conformation; D017785:Photons; D013057:Spectrum Analysis
D005456:Fluorescent Dyes; D049452:Green Fluorescent Proteins
GFP chromophore analogues; fluorescence imaging; fluorescent dyes; fluorescent probes; two-photon dyes