Flow-through sensor array applied to cytotoxicity assessment in cell cultures for drug-testing purposes.
The viability of cells cultured in microsystems for drug screening purposes is usually tested with a variety of colorimetric/fluorescent methods. In this work we propose an alternative way of assessing cell viability-flow-through sensor array that can be connected in series with cell microbioreactors as compatible detection system. It is shown, that the presented device is capable of cytotoxic effect detection and estimation of cell viability after treatment with 1,4-dioxane and 5-fluorouracil, which proves that it can be used for truly non-invasive, fast, reliable, continuous cell culture monitoring in microscale.
Warsaw University of Technology, Faculty of Chemistry, Noakowskiego 3, 00-664 Warsaw, Poland.
Emilia
E
Witkowska Nery
Elżbieta
E
Jastrzębska
Kamil
K
Żukowski
Wojciech
W
Wróblewski
Michał
M
Chudy
Patrycja
P
Ciosek
D000964:Antimetabolites, Antineoplastic / Q000494:pharmacology; D015374:Biosensing Techniques / Q000295:instrumentation; D018929:Cell Culture Techniques / Q000295:instrumentation*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects*; D004146:Dioxanes / Q000494:pharmacology; D004353:Drug Evaluation, Preclinical / Q000295:instrumentation*; D004867:Equipment Design; D005472:Fluorouracil / Q000494:pharmacology; D006801:Humans; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*
D000964:Antimetabolites, Antineoplastic; D004146:Dioxanes; C025223:1,4-dioxane; D005472:Fluorouracil
Cell culture; Cytotoxicity; Flow analysis; Microsystem; Sensor array