Changes in the expression of Heat Shock Proteins in ovaries from bovines with cystic ovarian disease induced by ACTH.
Cystic ovarian disease (COD), which is considered one of the most important causes of reproductive failure in dairy cattle, induces intraovarian changes in the expression of numerous genes. The purpose of this study was to analyze the changes in the expression of Heat Shock Proteins (HSPs) in ovaries from bovines with cystic ovarian disease induced by ACTH. Immunoreactivity for Heat Shock Proteins (HSPs) in ovaries of cows with induced COD showed differential expression patterns in growing follicles from the control group. The immunopositive area for Hsp27 and Hsp60 in granulosa cells showed significant differences between tertiary follicles from normal cycling animals and those from animals with induced COD. The cysts showed increased Hsp27 immunostaining in theca cells in relation to tertiary follicles from normal cycling cows. Hsp70 immunostaining was more intense in cystic follicles than in other follicular categories from animals with induced COD, in both granulosa and theca cells. In granulosa cells, tertiary follicles from the control group showed higher levels of Hsp90 than cysts. These results demonstrate that there are differences in HSP protein expression when COD is induced. In fact, HSP expression would be part of the functional response to the changes in hormones and neurotransmitters induced by stress, indicating that HSPs can control hormonal functions and vice versa.
Laboratorio de Biología Celular y Molecular Aplicada, Facultad de Ciencias Veterinarias, Universidad Nacional del Litoral, Argentina; Instituto de Ciencias Veterinarias del Litoral (ICiVet-Litoral), Consejo Nacional de Investigaciones Científicas y Tecnológicas, (CONICET), Argentina.
Melisa M L
MM
Velázquez
Natalia R
NR
Salvetti
Ayelen N
AN
Amweg
Pablo U
PU
Díaz
Valentina
V
Matiller
Hugo H
HH
Ortega
D000324:Adrenocorticotropic Hormone / Q000494:pharmacology*; D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000139:chemically induced / Q000378:metabolism*; D018834:Chaperonin 60 / Q000096:biosynthesis / Q000502:physiology; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects / Q000502:physiology; D006107:Granulosa Cells / Q000187:drug effects / Q000378:metabolism; D055551:HSP27 Heat-Shock Proteins / Q000096:biosynthesis / Q000502:physiology; D006360:Heat-Shock Proteins / Q000096:biosynthesis* / Q000502:physiology; D010048:Ovarian Cysts / Q000139:chemically induced / Q000378:metabolism / Q000662:veterinary*; D006080:Ovarian Follicle / Q000187:drug effects / Q000378:metabolism; D010053:Ovary / Q000187:drug effects / Q000378:metabolism*; D013799:Theca Cells / Q000187:drug effects / Q000378:metabolism
D018834:Chaperonin 60; D055551:HSP27 Heat-Shock Proteins; D006360:Heat-Shock Proteins; D000324:Adrenocorticotropic Hormone
Bovine; Cystic ovarian disease (COD); HSP; Ovary