Early exercise training improves ischemic outcome in rats by cerebral hemodynamics.
This study examined whether very early initiated physical rehabilitation (VEIPR), as a recommended therapy for postischemia, could improve motor performance and cerebral blood flow (CBF). Adult male rats with ischemic injury caused by middle cerebral artery occlusion (MCAO) were trained to run on a treadmill for 30min per day at 12m/min. Through such exercise training for 3 days, the ischemic rats exhibited increased motor function and decreased infarct volume, as measured by a behavioral score and 2,3,5-triphenyltetrazolium chloride (TTC) staining method, as well as accelerated CBF, as detected with laser speckle imaging (LSI). Furthermore, to determine whether the observed improved CBF provided the protective factor for motor function recovery, we investigated the apoptosis of ischemic rat brain microvascular endothelial cells (rBMECs), which accepted the mechanical force of CBF directly, under flow intervention. The findings indicated that a modest flow decreased cell apoptosis in the ischemic condition and that this effect is magnitude dependent, as excessive flow increased apoptosis.
Department of Rehabilitation, Huashan Hospital, Fudan University, Shanghai 200040, China.
Shan
S
Tian
Youcan
Y
Zhang
Song
S
Tian
Xiaojiao
X
Yang
Kewei
K
Yu
Yuling
Y
Zhang
Xiafeng
X
Shen
Liping
L
Zhang
Yu
Y
Sun
Hongyu
H
Xie
Zhijie
Z
He
Zhenzhen
Z
Guo
Jie
J
Jia
Yi
Y
Wu
Yulong
Y
Bai
Yulian
Y
Zhu
Yong
Y
Cheng
Xinggang
X
Wang
Junfa
J
Wu
Nianhong
N
Wang
Huixian
H
Yu
Yongshan
Y
Hu
D000818:Animals; D017209:Apoptosis; D001921:Brain / Q000098:blood supply* / Q000503:physiopathology*; D002545:Brain Ischemia / Q000628:therapy*; D042783:Endothelial Cells / Q000473:pathology; D005081:Exercise Therapy; D006439:Hemodynamics; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D012039:Regional Blood Flow; D016896:Treatment Outcome
Apoptosis; Brain microvascular endothelial cells; Cerebral blood flow; Ischemic stroke; Very early initiated physical rehabilitation