Immobilization of Candida rugosa lipase on superparamagnetic Fe3O4 nanoparticles for biocatalysis in low-water media.
A simple immobilization method for Candida rugosa lipase on superparamagnetic Fe3O4 nanoparticles is described. The Fe3O4 nanoparticles were coated with PEI and Candida rugosa lipase was adsorbed on these particles via electrostatic interactions. The immobilization resulted in marginal simultaneous purification. However, the immobilized preparation showed 110× higher transesterification activity in low-water media. It was also efficient in kinetic resolution of (±)-1-phenylethanol with eep of 99 % and E = 412 within 24 h.
Department of Chemistry, Indian Institute of Technology Delhi, New Delhi, India.
Joyeeta
J
Mukherjee
Kusum
K
Solanki
Munishwar Nath
MN
Gupta
D000327:Adsorption; D055162:Biocatalysis; D002175:Candida / Q000201:enzymology*; D057075:Enzyme Assays; D004800:Enzymes, Immobilized / Q000737:chemistry; D004951:Esterification; D052203:Ferrosoferric Oxide / Q000737:chemistry*; D005656:Fungal Proteins / Q000737:chemistry*; D008049:Lipase / Q000737:chemistry*; D060328:Magnetic Phenomena; D053758:Nanoparticles / Q000737:chemistry*; D011485:Protein Binding; D012996:Solutions; D014867:Water / Q000737:chemistry
D004800:Enzymes, Immobilized; D005656:Fungal Proteins; D012996:Solutions; D014867:Water; D008049:Lipase; D052203:Ferrosoferric Oxide