Evaluating the antioxidative activity of diselenide containing compounds in human blood.
This study was designed to determine and compare the antioxidant effects of synthetic organoselenium compounds. In experimental trials three different diselenides were used: bis(2-hydroxyphenyl) diselenide, bis{[2-(4-hydroxybenzyl)imino]phenyl} diselenide and bis[2-(4-methylphenylsulfonylamino)phenyl] diselenide. The compounds were screened for antioxidant activities in human blood under oxidation stress conditions. Oxidative stress was induced in vitro in human blood platelet samples and in plasma by 0.1 mM peroxynitrite (ONOO(-)) or by Fe(2+). In experimental trials the levels of chosen oxidative stress markers (TBARS, O2(-), and protein carbonyl groups) were significantly decreased by the action of the tested compounds. The antioxidative properties and the changes in proteins and lipids in the presence of new synthesized selenoorganic compounds were studied in vitro and compared with activity of ebselen (2-phenyl-1,2-benzisoselenazol-3(2H)-one)--a classical antioxidant, well known as the most important glutathione peroxidase mimetic agent. Our results indicate that the tested diselenides have distinctly protective effects against oxidative alterations of biomolecules caused by ONOO(-) and Fe(2+) in blood platelets and in plasma. Therefore it seems that not only ebselen with a wide spectrum of therapeutic actions but also other organoselenium compounds can be considered in the future as active pharmacological agents.
Department of General Biochemistry, Faculty of Biology and Environmental Protection, University of Lodz, Pomorska 141/143, 90-236 Lodz, Poland. Electronic address: juszczak@biol.uni.lodz.pl.
Joanna
J
Saluk
Michał
M
Bijak
Paweł
P
Nowak
Barbara
B
Wachowicz
D000975:Antioxidants / Q000032:analysis / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D001792:Blood Platelets / Q000737:chemistry*; D004305:Dose-Response Relationship, Drug; D005296:Ferrous Compounds / Q000037:antagonists & inhibitors / Q000494:pharmacology; D006801:Humans; D016566:Organoselenium Compounds / Q000097:blood* / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects; D030421:Peroxynitrous Acid / Q000037:antagonists & inhibitors / Q000494:pharmacology; D013329:Structure-Activity Relationship
D000975:Antioxidants; D005296:Ferrous Compounds; D016566:Organoselenium Compounds; D030421:Peroxynitrous Acid
Antioxidant; Diselenide derivatives; Ebselen