Catalog of mRNA expression patterns for DNA methylating and demethylating genes in developing mouse lower urinary tract.
The mouse prostate develops from a component of the lower urinary tract (LUT) known as the urogenital sinus (UGS). This process requires androgens and signaling between mesenchyme and epithelium. Little is known about DNA methylation during prostate development, including which factors are expressed, whether their expression changes over time, and if DNA methylation contributes to androgen signaling or influences signaling between mesenchyme and epithelium. We used in situ hybridization to evaluate the spatial and temporal expression pattern of mRNAs which encode proteins responsible for establishing, maintaining or remodeling DNA methylation. These include DNA methyltransferases, DNA deaminases, DNA glycosylases, base excision repair and mismatch repair pathway members. The mRNA expression patterns were compared between male and female LUT prior to prostatic bud formation (14.5 days post coitus (dpc)), during prostatic bud formation (17.5 dpc) and during prostatic branching morphogenesis (postnatal day (P) 5). We found dramatic changes in the patterns of these mRNAs over the course of prostate development and identified examples of sexually dimorphic mRNA expression. Future investigation into how DNA methylation patterns are established, maintained and remodeled during the course of embryonic prostatic bud formation may provide insight into prostate morphogenesis and disease.
Department of Comparative Biosciences, University of Wisconsin-Madison, 1656 Linden Dr., Madison, WI 53706, USA.
Kimberly P
KP
Keil
Helene M
HM
Altmann
Vatsal
V
Mehta
Lisa L
LL
Abler
Erik A
EA
Elton
Chad M
CM
Vezina
D000071479:APOBEC-1 Deaminase; D000251:Adenosine Triphosphatases / Q000235:genetics / Q000378:metabolism; D000818:Animals; D003564:Cytidine Deaminase / Q000235:genetics / Q000378:metabolism; D004248:DNA (Cytosine-5-)-Methyltransferases / Q000235:genetics / Q000378:metabolism; D045647:DNA Glycosylases / Q000235:genetics / Q000378:metabolism; D045643:DNA Repair Enzymes / Q000235:genetics / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D004706:Endodeoxyribonucleases / Q000235:genetics / Q000378:metabolism; D005260:Female; D018507:Gene Expression Regulation, Developmental; D015971:Gene Expression Regulation, Enzymologic; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D000070976:Mismatch Repair Endonuclease PMS2; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D059467:Transcriptome; D014551:Urinary Tract / Q000196:embryology / Q000378:metabolism*; D000098830:AICDA (Activation-Induced Cytidine Deaminase)
C514205:Chd2 protein, mouse; D004268:DNA-Binding Proteins; D012333:RNA, Messenger; D004248:DNA (Cytosine-5-)-Methyltransferases; D004706:Endodeoxyribonucleases; D045647:DNA Glycosylases; D000098830:AICDA (Activation-Induced Cytidine Deaminase); D000071479:APOBEC-1 Deaminase; C000605090:Apobec1 protein, mouse; D003564:Cytidine Deaminase; D000251:Adenosine Triphosphatases; C499533:Pms2 protein, mouse; D000070976:Mismatch Repair Endonuclease PMS2; D045643:DNA Repair Enzymes
AR; DNA demethylation; DNA methylation; Epigenetics; IHC; ISH; LUT; Lower urinary tract; P; Prostate; UGS; Urogenital sinus; androgen receptor; days post coitus; dpc; immunohistochemistry; in situ hybridization; lower urinary tract; postnatal day; urogenital sinus