Lectin interactions on surface-grafted glycostructures: influence of the spatial distribution of carbohydrates on the binding kinetics and rupture forces.
We performed quantitative analysis of the binding kinetics and affinity of carbohydrate-lectin binding and correlated them directly with the molecular and structural features of ligands presented at the nanoscale within the glycocalyx mimicking layers on surfaces. The surface plasmon resonance analysis identified that the mode of binding changed from multivalent to monovalent, which resulted in a near 1000-fold change in the equilibrium association constant, by varying the spatial distribution of carbohydrate ligands within the surface-grafted polymer layer. We identified, for the first time, that the manner in which the ligands presented on the surface has great influence on the binding at the first stage of bivalent chelating, not on the binding at the second stage. The rupture forces measured by atomic force microscope force spectroscopy also indicated that the mode of binding between lectin and ligands changed from multiple to single with variation in the ligand presentation. The dependence of lectin binding on the glycopolymer composition and grafting density is directly correlated with the nanoscale presentation of ligands on a surface, which is a determining factor in controlling the clustering and statistical effects contributing to the enhanced binding.
Centre for Blood Research and Department of Pathology & Laboratory Medicine, University of British Columbia, Vancouver, British Columbia, Canada.
Kai
K
Yu
A Louise
AL
Creagh
Charles A
CA
Haynes
Jayachandran N
JN
Kizhakkedathu
D002241:Carbohydrates / Q000737:chemistry*; D007700:Kinetics; D037102:Lectins / Q000737:chemistry*; D008024:Ligands; D018625:Microscopy, Atomic Force; D020349:Surface Plasmon Resonance; D013499:Surface Properties
D002241:Carbohydrates; D037102:Lectins; D008024:Ligands