Insect protein digestion improves purity of Steinernema carpocapsae in vitro culture and reduces culture period.
Insect protein, used for in vitro culture media for entomopathogenic nematode, produces nematodes of high quality. However, the time-consuming culture and poor purity of nematodes hinder the commercial application of insect protein media. We show that hydrolyzed insect protein improves nematode purity in in vitro culture. The results revealed that nematode purity was increased by more than 90 %, and the culture period was reduced by 6 days. Estimated economic efficiency of using hydrolyzed insect protein medium was increased by 44.25 % over that obtained with non-hydrolyzed insect medium.
Department of Plant Pathology, China Agricultural University, Beijing, 100193, People's Republic of China.
Cuiling
C
Cao
Heng
H
Jian
Aiqin
A
Zhao
Xia
X
Jiang
Qian
Q
Liu
D000818:Animals; D003470:Culture Media / Q000737:chemistry*; D019476:Insect Proteins / Q000378:metabolism*; D010274:Parasitology / Q000379:methods*; D059748:Proteolysis; D017168:Rhabditida / Q000254:growth & development* / Q000378:metabolism*
D003470:Culture Media; D019476:Insect Proteins