Critical role of interleukin-5 in the development of a mite antigen-induced chronic bronchial asthma model.
OBJECTIVE
Asthma is associated with eosinophilic airway inflammation and characterized by enhanced airway sensitivity. Interleukin (IL)-5 plays an important role in the pathogenesis of asthma. The involvement of IL-5 receptor-mediated cellular signals in the pathogenesis of a mite antigen-induced chronic asthma model was investigated.


METHODS
In this study, 48 female C57BL/6J (WT) mice and IL-5 receptor-deficient (IL-5RKO) mice were used.


METHODS
Mite antigen (50 μl) was intranasally administered 13 times to WT and IL-5RKO mice.


METHODS
Airway hypersensitivity (Mch PC200) and specific antigen exposure tests were performed, and lung tissue, bronchoalveolar lavage fluid (BALF), and blood were collected to investigate the asthma pathology and differences in the local pulmonary levels of cytokines and chemokines.


RESULTS
Airway sensitivity was enhanced and antigen-specific airway resistance was increased in WT mice. In addition, the number of eosinophils and Th2 cytokine levels in the BALF were increased. In contrast, IL-5RKO mice did not acquire the asthma pathology, such as antigen-specific airway resistance and eosinophilic airway inflammation. Mch PC200 was significantly correlated with cysteinyl leukotriene levels in WT mice.


CONCLUSIONS
These findings suggested that both IL-5 induced eosinophils and cysteinyl leukotrienes are involved in the pathology of this mite antigen-induced chronic asthma model.
Department of Respiratory Medicine, Kawasaki Medical School, 577 Matsushima, Kurashiki, Okayama, 701-0192, Japan, shimizu@med.kawasaki-m.ac.jp.
Hiroki
H
Shimizu
Yasushi
Y
Obase
Shigeki
S
Katoh
Keiji
K
Mouri
Yoshihiro
Y
Kobashi
Mikio
M
Oka
D000403:Airway Resistance / Q000276:immunology; D000485:Allergens / Q000276:immunology*; D000818:Animals; D039741:Antigens, Dermatophagoides / Q000276:immunology*; D001249:Asthma / Q000209:etiology / Q000276:immunology* / Q000503:physiopathology; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology / Q000276:immunology; D016207:Cytokines / Q000276:immunology; D004804:Eosinophils / Q000166:cytology; D005260:Female; D007073:Immunoglobulin E / Q000097:blood; D007074:Immunoglobulin G / Q000097:blood; D015848:Interleukin-5 / Q000276:immunology*; D007958:Leukocyte Count; D015289:Leukotrienes / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D053649:Receptors, Interleukin-5 / Q000235:genetics / Q000276:immunology
D000485:Allergens; D039741:Antigens, Dermatophagoides; D016207:Cytokines; D007074:Immunoglobulin G; D015848:Interleukin-5; D015289:Leukotrienes; D053649:Receptors, Interleukin-5; D007073:Immunoglobulin E