Application of multiple parallel perfused microbioreactors: Synthesis, characterization and cytotoxicity testing of the novel rare earth complexes with indole acid as a ligand.
Three novel complexes, [La(phen)2(IAA)2]·NO3 (1), [Sm(phen)2(IAA)2]·NO3 (2) and [Sm(IBA)3(phen)]·phen·HNO3·H2O (3) (phen: 1,10-phenanthroline, IAA: indole-3-acetic acid, IBA: indole-3-butyric acid), were synthesized and characterized with spectroscopy (infrared and UV-visible), X-ray crystal diffraction and elemental analysis. Structural analysis revealed that each lanthanide atom in complexes 1-3 held a distorted tricapped trigonal prism geometry in a nine-coordinate mode. There were two types of coordination modes of the IAA ligand in complexes 1 and 2: a μ2-η(1):η(2) bridging mode linking two lanthanide atoms and a μ2-η(1):η(1) double monodentate bridging mode. There were three types of coordination modes of the IBA ligand: a μ2-η(1):η(1) double monodentate bridging mode, a μ1-η(2) bridging mode and a μ2-η(1):η(2) bridging mode linking two lanthanide atoms. Adjacent Sm atoms were linked via the μ2-bridging carboxylate groups of the IBA ligands to generate a binuclear building unit. The biological activity of the complexes was evaluated in human adipose tissue-derived stem cells (hADSCs) and Chang liver cells using a multiple parallel perfused microbioreactor. The results showed that cytotoxicity increased as the concentrations of complexes 1-3 increased.
College of Chemistry and Chemical Engineering, Liaoning Normal University, No. 850, Huanghe Road, Dalian 116029, PR China; Regenerative Medicine Centre, First Affiliated Hospital of Dalian Medical University, No. 222 Zhongshan Road, Dalian 116011, PR China.
Qing-Lin
QL
Guan
Yong-Heng
YH
Xing
Jing
J
Liu
Wen-Juan
WJ
Wei
Rui
R
Zhang
Xuan
X
Wang
Feng-Ying
FY
Bai
D000273:Adipose Tissue / Q000166:cytology; D017209:Apoptosis / Q000187:drug effects; D019149:Bioreactors; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D056831:Coordination Complexes / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology*; D018360:Crystallography, X-Ray; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007210:Indoleacetic Acids / Q000737:chemistry*; D007211:Indoles / Q000737:chemistry*; D028581:Lanthanoid Series Elements / Q000737:chemistry*; D008099:Liver / Q000166:cytology; D008958:Models, Molecular; D015394:Molecular Structure; D010477:Perfusion; D013053:Spectrophotometry; D013234:Stem Cells / Q000166:cytology / Q000187:drug effects; D014961:X-Ray Diffraction
D056831:Coordination Complexes; D007210:Indoleacetic Acids; D007211:Indoles; D028581:Lanthanoid Series Elements; C014612:indolebutyric acid; C030737:indoleacetic acid
Cytotoxicity; Indole acid; Lanthanide complexes; Microbioreactors; Molecular structure