[MMP-3 and MMP-8 levels in patients with chronic periodontitis before and after nonsurgical periodontal therapy].
Immune-inflammatory processes are trigged in chronic periodontitis (CP), where matrix metalloproteinases (MMPs) are released and involved in the degradation of the extracellular matrix components that can be detected in the gingival crevicular fluid (GCF). The purpose of the study was to determine the levels of MMP-3 and MMP-8 in GCF, before and after nonsurgical periodontal treatment (NSPT), to evaluate disease activity and therapy response. Eleven patients with PC and eleven healthy controls were selected. Clinical measurements to evaluate gingival index (GI), plaque index (PI), probing depth (PD) and clinical attachment loss (CAL) were made in all the teeth of each individual and in six sites per tooth. GCF samples were taken from one tooth per quadrant, with a pocket depth > or =4 mm and a clinical attachment loss > or =5 mm, and the levels of MMP-3 and MMP-8 measured using an ELISA test. Statistically significant differences in clinical parameters were observed (p < 0.05) between patients with CP and control groups before the periodontal treatment, with significant decrease in all indexes after the NSPT. The initial concentrations of MMP-3 and MMP-8 were significantly higher than those obtained after the NSPT and in the control group, without observing a correlation between the clinical parameters and the levels of MMPs. Increased levels of MMP-3 and MMP-8 in the GCF of patients with PC declined significantly after NSPT, and the difference between the levels in healthy individuals and patients, suggests the important participation of these MMPs in tissue destruction in PC disease..
Instituto de Investigaciones Odontológicas Raúl Vincentelli, Facultad de Odontología, Universidad Central de Venezuela, Caracas, Venezuela. adriromeror@gmail.com
Adriana M
AM
Romero
Patrizzia
P
Mastromatteo-Alberga
Laura
L
Escalona
María
M
Correnti
D000328:Adult; D015415:Biomarkers; D002908:Chronic Disease; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005883:Gingival Crevicular Fluid / Q000201:enzymology*; D006801:Humans; D008297:Male; D019278:Matrix Metalloproteinase 3 / Q000032:analysis*; D020784:Matrix Metalloproteinase 8 / Q000032:analysis*; D008875:Middle Aged; D010512:Periodontal Index; D010518:Periodontitis / Q000201:enzymology* / Q000628:therapy
D015415:Biomarkers; C511768:MMP3 protein, human; D019278:Matrix Metalloproteinase 3; C488762:MMP8 protein, human; D020784:Matrix Metalloproteinase 8