The novel structure of the core oligosaccharide backbone of the lipopolysaccharide from the Plesiomonas shigelloides strain CNCTC 80/89 (serotype O13).
The new structure of the core oligosaccharide of Plesiomonas shigelloides CNCTC 80/89 (serotype O13) lipopolysaccharide has been investigated by chemical methods, (1)H and (13)C NMR spectroscopy and matrix-assisted laser-desorption/ionization time of flight (MALDI-TOF). It was concluded that the core oligosaccharide of P. shigelloides CNCTC 80/89 is a nonasaccharide with the following structure: The position of glycine was determined by MALDI-TOF MS/MS analyses.
Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, R. Weigla 12, PL-53-114 Wroclaw, Poland. Electronic address: Marta.Kaszowska@iitd.pan.wroc.pl.
Marta
M
Kaszowska
Wojciech
W
Jachymek
Tomasz
T
Niedziela
Sabina
S
Koj
Lennart
L
Kenne
Czeslaw
C
Lugowski
D002240:Carbohydrate Sequence; D008070:Lipopolysaccharides / Q000737:chemistry*; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry*; D016981:Plesiomonas / Q000737:chemistry*
D008070:Lipopolysaccharides; D009844:Oligosaccharides
CNCTC; COSY; Core oligosaccharide; Czech National Collection of Type Cultures; DEPT; GC; Glycine; HMBC; HSQC; LPS; Lipopolysaccharide; MALDI-TOF; MALDI-TOF MS/MS; MS; NMR; NOESY; OS; Plesiomonas shigelloides; TOCSY; correlated spectroscopy; distortionless enhancement by polarization transfer; gas chromatography; heteronuclear multiple bond correlation; heteronuclear single quantum coherence; lipopolysaccharide; mass spectrometry; matrix-assisted laser-desorption/ionization time-of-flight; nuclear Overhauser effect spectroscopy; nuclear magnetic resonance; oligosaccharide; total correlation spectroscopy