An in vitro study reveals nutraceutical properties of Ananas comosus (L.) Merr. var. Mauritius fruit residue beneficial to diabetes.
BACKGROUND
Rapid urbanisation and nutritional transition is fuelling the increased global incidence of type 2 diabetes. Pineapple fruit residue was explored for its nutraceutical properties as an alternative or adjunct to currently available treatment regime. Ethyl acetate and methanolic extracts of pineapple fruit residue were evaluated for anti-diabetic activity in cell free and cell based systems. Specifically, we assessed: (1) antioxidant potential, (2) anti-glycation potential, (3) carbohydrate digestive enzyme inhibition, and (4) lipid accumulation and glycerol-3-phosphate dehydrogenase activity in differentiating 3T3-L1 cells.


RESULTS
The active components in the ethyl acetate and methanolic extracts were identified as sinapic acid, daucosterol, 2-methylpropanoate, 2,5-dimethyl-4-hydroxy-3(2H)-furanone, methyl 2-methylbutanoate and triterpenoid ergosterol using DART/HRMS and ESI/HRMS. Micronutrient analysis revealed the presence of magnesium, potassium and calcium. Adipogenic potential, anti-glycation property of the ethyl acetate extract, and DNA damage protection capacity of the methanolic extract are promising.


CONCLUSIONS
Results from this study clearly indicate that pineapple fruit residue could be utilised as a nutraceutical against diabetes and related complications.
Agroprocessing and Natural Products Division, Council of Scientific and Industrial Research, National Institute for Interdisciplinary Science and Technology (CSIR-NIIST), Thiruvananthapuram-, 695019, Kerala, India.
Mariam Philip
MP
Riya
Kalathookunnel Antony
KA
Antu
Thankamony
T
Vinu
Karuvakandy Chandrasekharan
KC
Chandrakanth
Karunakaran Sasikala
KS
Anilkumar
Kozhiparambil Gopalan
KG
Raghu
D041721:3T3-L1 Cells; D052438:Adipocytes, White / Q000166:cytology / Q000378:metabolism*; D050156:Adipogenesis; D031230:Ananas / Q000737:chemistry*; D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000191:economics / Q000302:isolation & purification; D019587:Dietary Supplements; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000191:economics / Q000302:isolation & purification; D005525:Food-Processing Industry / Q000191:economics; D005638:Fruit / Q000737:chemistry*; D005993:Glycerolphosphate Dehydrogenase / Q000037:antagonists & inhibitors / Q000378:metabolism; D065089:Glycoside Hydrolase Inhibitors / Q000737:chemistry / Q000191:economics / Q000302:isolation & purification; D006031:Glycosylation; D007004:Hypoglycemic Agents / Q000737:chemistry / Q000191:economics / Q000302:isolation & purification*; D007194:India; D007220:Industrial Waste / Q000032:analysis* / Q000191:economics; D008082:Lipotropic Agents / Q000737:chemistry / Q000191:economics / Q000302:isolation & purification; D051379:Mice; D010936:Plant Extracts / Q000737:chemistry / Q000191:economics / Q000302:isolation & purification*; D012997:Solvents / Q000737:chemistry; D000516:alpha-Amylases / Q000037:antagonists & inhibitors / Q000378:metabolism
D000975:Antioxidants; D004791:Enzyme Inhibitors; D065089:Glycoside Hydrolase Inhibitors; D007004:Hypoglycemic Agents; D007220:Industrial Waste; D008082:Lipotropic Agents; D010936:Plant Extracts; D012997:Solvents; D005993:Glycerolphosphate Dehydrogenase; D000516:alpha-Amylases
Ananas comosus (L.) Merr. var. Mauritius; adipogenesis; anti-glycation; antioxidant; bromeliaceae; pineapple fruit residue; α-glucosidase