Performance and microbial community analysis of the anaerobic reactor with coke oven gas biomethanation and in situ biogas upgrading.
A new method for simultaneous coke oven gas (COG) biomethanation and in situ biogas upgrading in anaerobic reactor was developed in this study. The simulated coke oven gas (SCOG) (92% H2 and 8% CO) was injected directly into the anaerobic reactor treating sewage sludge through hollow fiber membrane (HFM). With pH control at 8.0, the added H2 and CO were fully consumed and no negative effects on the anaerobic degradation of sewage sludge were observed. The maximum CH4 content in the biogas was 99%. The addition of SCOG resulted in enrichment and dominance of homoacetogenetic genus Treponema and hydrogenotrophic genus Methanoculleus in the liquid, which indicated that H2 were converted to methane by both direct (hydrogenotrophic methanogenesis) and indirect (homoacetogenesis+aceticlastic methanogenesis) pathways in the liquid. However, the aceticlasitic genus Methanosaeta was dominant for archaea in the biofilm on the HFM, which indicated indirect (homoacetogenesis+aceticlastic methanogenesis) H2 conversion pathway on the biofilm.
State Key Laboratory of Pollution Control and Resources Reuse, Tongji University, 1239 Siping Road, Shanghai 200092, PR China; Department of Environmental Engineering, Technical University of Denmark, DK-2800 Kgs Lyngby, Denmark.
Wen
W
Wang
State Key Laboratory of Pollution Control and Resources Reuse, Tongji University, 1239 Siping Road, Shanghai 200092, PR China. Electronic address: sally.xieli@gmail.com.
Li
L
Xie
Department of Environmental Engineering, Technical University of Denmark, DK-2800 Kgs Lyngby, Denmark. Electronic address: gangl@env.dtu.dk.
Gang
G
Luo
State Key Laboratory of Pollution Control and Resources Reuse, Tongji University, 1239 Siping Road, Shanghai 200092, PR China.
Qi
Q
Zhou
Department of Environmental Engineering, Technical University of Denmark, DK-2800 Kgs Lyngby, Denmark. Electronic address: iria@env.dtu.dk.
Irini
I
Angelidaki
D000393:Air Pollutants / Q000737:chemistry*; D000693:Anaerobiosis; D018441:Biofilms; D056804:Biofuels; D019149:Bioreactors; D002248:Carbon Monoxide / Q000737:chemistry; D003077:Coke; D006863:Hydrogen-Ion Concentration; D007220:Industrial Waste; D008697:Methane / Q000737:chemistry; D019638:Methanomicrobiaceae / Q000378:metabolism; D012336:RNA, Ribosomal, 16S / Q000235:genetics / Q000378:metabolism; D012722:Sewage / Q000737:chemistry; D013997:Time Factors; D014208:Treponema / Q000378:metabolism; D014865:Waste Disposal, Fluid
D000393:Air Pollutants; D056804:Biofuels; D003077:Coke; D007220:Industrial Waste; D012336:RNA, Ribosomal, 16S; D012722:Sewage; D002248:Carbon Monoxide; D008697:Methane
Anaerobic digestion; Biogas upgrading; Biomethanation; Coke oven gas