Central region component1, a novel synaptonemal complex component, is essential for meiotic recombination initiation in rice.
In meiosis, homologous recombination entails programmed DNA double-strand break (DSB) formation and synaptonemal complex (SC) assembly coupled with the DSB repair. Although SCs display extensive structural conservation among species, their components identified are poorly conserved at the sequence level. Here, we identified a novel SC component, designated central region component1 (CRC1), in rice (Oryza sativa). CRC1 colocalizes with ZEP1, the rice SC transverse filament protein, to the central region of SCs in a mutually dependent fashion. Consistent with this colocalization, CRC1 interacts with ZEP1 in yeast two-hybrid assays. CRC1 is orthologous to Saccharomyces cerevisiae pachytene checkpoint2 (Pch2) and Mus musculus THYROID receptor-interacting protein13 (TRIP13) and may be a conserved SC component. Additionally, we provide evidence that CRC1 is essential for meiotic DSB formation. CRC1 interacts with homologous pairing aberration in rice meiosis1 (PAIR1) in vitro, suggesting that these proteins act as a complex to promote DSB formation. PAIR2, the rice ortholog of budding yeast homolog pairing1, is required for homologous chromosome pairing. We found that CRC1 is also essential for the recruitment of PAIR2 onto meiotic chromosomes. The roles of CRC1 identified here have not been reported for Pch2 or TRIP13.
State Key Laboratory of Plant Genomics and Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
Chunbo
C
Miao
Ding
D
Tang
Honggen
H
Zhang
Mo
M
Wang
Yafei
Y
Li
Shuzhu
S
Tang
Hengxiu
H
Yu
Minghong
M
Gu
Zhukuan
Z
Cheng
D032461:Chromosomes, Plant / Q000235:genetics; D003001:Cloning, Molecular; D053903:DNA Breaks, Double-Stranded; D018076:DNA, Complementary / Q000235:genetics; D008540:Meiosis / Q000235:genetics*; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D012275:Oryza / Q000166:cytology* / Q000235:genetics* / Q000378:metabolism; D010641:Phenotype; D051479:Plant Infertility / Q000235:genetics; D010940:Plant Proteins / Q000378:metabolism*; D011485:Protein Binding; D021381:Protein Transport; D011995:Recombination, Genetic; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D020539:Sequence Analysis, Protein; D013573:Synaptonemal Complex / Q000378:metabolism*
D018076:DNA, Complementary; D010940:Plant Proteins