Reassortment and migration analysis of Crimean-Congo haemorrhagic fever virus.
Crimean-Congo haemorrhagic fever virus (CCHFV) is a tick-borne virus with high pathogenicity to humans. CCHFV contains a three-segment [small (S), medium (M) and large (L)] genome and is prone to reassortment. Investigation of identified reassortment events can yield insight into the evolutionary history of the virus, while migration events reflect its geographical dissemination. While many studies have already considered these issues, they have investigated small numbers of isolates and lack statistical support for their findings. Here, we consider a larger set of 30 full genomes to investigate reassortment using recombination methods, as well as two sets of partial S segments comprising 393 isolates, reflecting a broader geographical range, to investigate migration events. Phylogenetic analysis revealed that the S segment showed strong geographical subdivision, but this was less apparent in the M and L segments. A total of 16 reassortment events with 22 isolates were identified with strong statistical support. Migration analysis on the partial S segments identified both long- and short-range migration events that spanned the entire geographical region in which the CCHFV has been isolated, reflecting the complex processes associated with the dissemination of the virus.
State Key Laboratory of Virology and China Center for Virus Culture Collection, Chinese Academy of Sciences, Wuhan 430071, PR China.
Zhaorui
Z
Zhou
State Key Laboratory of Virology and China Center for Virus Culture Collection, Chinese Academy of Sciences, Wuhan 430071, PR China.
Fei
F
Deng
State Key Laboratory of Virology and China Center for Virus Culture Collection, Chinese Academy of Sciences, Wuhan 430071, PR China.
Na
N
Han
State Key Laboratory of Virology and China Center for Virus Culture Collection, Chinese Academy of Sciences, Wuhan 430071, PR China.
Hualin
H
Wang
Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumuqi, Xinjiang 830046, PR China.
Surong
S
Sun
Center for Disease Control and Prevention of Xinjiang Uygur Autonomous Region, 4 Jianquan First Street, Urumuqi, Xinjiang 830046, PR China.
Yujiang
Y
Zhang
State Key Laboratory of Virology and China Center for Virus Culture Collection, Chinese Academy of Sciences, Wuhan 430071, PR China.
Zhihong
Z
Hu
State Key Laboratory of Virology and China Center for Virus Culture Collection, Chinese Academy of Sciences, Wuhan 430071, PR China.
Simon
S
Rayner
D025041:Animal Migration; D000818:Animals; D001717:Birds / Q000469:parasitology / Q000502:physiology*; D019295:Computational Biology / Q000379:methods; D016679:Genome, Viral / Q000235:genetics*; D003225:Hemorrhagic Fever Virus, Crimean-Congo / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D006479:Hemorrhagic Fever, Crimean / Q000635:transmission* / Q000821:virology; D006801:Humans; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics; D016865:Reassortant Viruses / Q000235:genetics*; D011995:Recombination, Genetic / Q000235:genetics; D013984:Tick Infestations / Q000469:parasitology* / Q000635:transmission; D017282:Tick-Borne Diseases / Q000635:transmission* / Q000821:virology; D013987:Ticks / Q000821:virology
D012367:RNA, Viral