Microfluidic chip integrated with flexible PDMS-based electrochemical cytosensor for dynamic analysis of drug-induced apoptosis on HeLa cells.
A novel microfluidic platform integrated with a flexible PDMS-based electrochemical cytosensor was developed for real-time monitoring of the proliferation and apoptosis of HeLa cells. The PDMS-gold film, which had a conductive smooth surface and was semi-transparent, facilitated electrochemical measurements and optical microscope observations. We observed distinct increases and decreases in peak current intensity, corresponding to cell proliferation in culture medium and apoptosis in the presence of an anticancer drug, respectively. This electrochemical analysis method permitted real-time, label-free monitoring of cell behavior, and the electrochemical results were confirmed with optical microscopy. The flexible microfluidic electrochemical platform presented here is suitable for on-site monitoring of cell behavior in microenvironments.
State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, PR China.
Jun-Tao
JT
Cao
Ying-Di
YD
Zhu
Rohit Kumar
RK
Rana
Jun-Jie
JJ
Zhu
D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D015374:Biosensing Techniques / Q000295:instrumentation*; D004129:Dimethylpolysiloxanes / Q000737:chemistry*; D004354:Drug Screening Assays, Antitumor / Q000295:instrumentation*; D055664:Electrochemical Techniques / Q000295:instrumentation; D004867:Equipment Design; D006367:HeLa Cells; D006801:Humans; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*
D000970:Antineoplastic Agents; D004129:Dimethylpolysiloxanes
Apoptosis; Dynamic analysis; Electrochemical cytosensing; Flexible PDMS; Microfluidic chip