Assessing competence of broccoli consumption on inflammatory and antioxidant pathways in restraint-induced models: estimation in rat hippocampus and prefrontal cortex.
A growing body of evidence advocated the protective and therapeutic potential of natural compounds and phytochemicals used in diets against pathological conditions. Herein, the outcome of dietary whole broccoli consumption prior to restraint stress has been investigated in the hippocampus and prefrontal cortex of male rats, two important regions involved in the processing of responses to stressful events. Interestingly, a region-specific effect was detected regarding some of antioxidant defense system factors: nuclear factor erythroid-derived 2-related factor 2 (Nrf-2) antioxidant pathway, mitochondrial prosurvival proteins involved in mitochondrial biogenesis, and apoptotic cell death proteins. Dietary broccoli supplementation modulated the restraint-induced changes towards a consistent overall protection in the hippocampus. In the prefrontal cortex, however, despite activation of most of the protective factors, presumably as an attempt to save the system against the stress insult, some detrimental outcomes such as induced malate dehydrogenase (MDA) level and cleaved form of caspase-3 were detectable. Such diversity may be attributed in one hand to the different basic levels and/or availability of defensive mechanisms within the two studied cerebral regions, and on the other hand to the probable dose-dependent and hormetic effects of whole broccoli. More experiments are essential to demonstrate these assumptions.
Physiology and Pharmacology Department, Medical School, Alborz University of Medical Sciences, Karaj, Iran. lkhalaj@yahoo.com
Leila
L
Khalaj
Sara Chavoshi
SC
Nejad
Marzieh
M
Mohammadi
Sadaf
S
Sarraf Zadeh
Marieh Hossein
MH
Pour
Ghorbangol
G
Ashabi
Fariba
F
Khodagholi
Abolhassan
A
Ahmadiani
D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D001937:Brassica; D004032:Diet; D006624:Hippocampus / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D007249:Inflammation / Q000178:diet therapy* / Q000473:pathology; D008291:Malate Dehydrogenase / Q000378:metabolism; D008297:Male; D053858:Metabolic Networks and Pathways; D051267:NF-E2-Related Factor 2 / Q000378:metabolism; D017397:Prefrontal Cortex / Q000378:metabolism / Q000473:pathology; D051381:Rats; D012149:Restraint, Physical; D013315:Stress, Psychological / Q000378:metabolism
D000975:Antioxidants; D051267:NF-E2-Related Factor 2; C495637:Nfe2l2 protein, rat; D008291:Malate Dehydrogenase