Isolation of mesenchymal stem-like cells in meningioma specimens.
Cells resembling bone marrow mesenchymal stem cells (BM-MSCs) have been isolated from glioma specimens; however, little is known about the existence of mesenchymal stem-like cells (MSLCs) in meningioma. Here, we hypothesized that cells similar to BM-MSCs exist in meningioma specimens and sought to investigate whether these putative meningioma stroma MSLCs (MS-MSLCs) could be isolated. To this end, we cultured fresh meningioma specimens using the same protocols as used previously to isolate BM-MSC. Cultured cells were analyzed for surface markers associated with BM-MSCs by fluorescence-activated cell sorting (FACS) and candidate cells were exposed to mesenchymal differentiation conditions. Possible locations of MS-MSLCs were determined by immunohistochemical analysis of sections of meningioma specimens. Spindle-shaped and, adherent cells similar to BM-MSCs were isolated in 2 of 20 meningioma specimens. FACS analysis showed that the surface markers of MS-MSLCs were similar to those of BM-MSCs and the chosen cells demonstrated an ability to differentiate into osteogenic, adipogenic and chondrogenic cells. The tumorigenicity of MS-MSLCs was tested by injection of these cells into the brain of athymic nude mice; no tumors were subsequently discovered. Immunohistochemical analyses indicated that CD105+ cells were closely associated with endothelial cells and pericytes in meningioma specimens. Our results established for the first time that cells similar to BM-MSCs exist in meningioma specimens. These cells, termed MS-MSLCs, could be one component of the meningioma cellular microenvironment.
Department of Neurosurgery, Severance Hospital, Yonsei University College of Medicine, Seoul, Republic of Korea.
Hyo-Yeol
HY
Lim
Kyung Min
KM
Kim
Bo Kyung
BK
Kim
Jin-Kyoung
JK
Shim
Ji-Hyun
JH
Lee
Yong-Min
YM
Huh
Se-Hoon
SH
Kim
Eui-Hyun
EH
Kim
Eun-Kyung
EK
Park
Kyu-Won
KW
Shim
Jong Hee
JH
Chang
Dong-Seok
DS
Kim
Sun Ho
SH
Kim
Yong-Kil
YK
Hong
Su-Jae
SJ
Lee
Seok-Gu
SG
Kang
D050156:Adipogenesis / Q000235:genetics; D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D001854:Bone Marrow Cells / Q000166:cytology* / Q000378:metabolism; D002454:Cell Differentiation / Q000235:genetics*; D002469:Cell Separation; D002478:Cells, Cultured; D020219:Chondrogenesis / Q000235:genetics; D000071063:Endoglin; D005434:Flow Cytometry; D006801:Humans; D008579:Meningioma / Q000235:genetics* / Q000473:pathology; D059630:Mesenchymal Stem Cells / Q000473:pathology*; D051379:Mice; D010012:Osteogenesis / Q000235:genetics; D011956:Receptors, Cell Surface / Q000378:metabolism
D015703:Antigens, CD; C064206:ENG protein, human; D000071063:Endoglin; D011956:Receptors, Cell Surface