Linkers, resins, and general procedures for solid-phase peptide synthesis.
This chapter describes the basic protocols for solid-phase peptide synthesis using the Fmoc group as the N (α)-protecting group (Fmoc-SPPS). The chapter introduces resins and their handling, choice of linkers, and the most common methods for peptide chain assembly. The proper choice of resins and linkers for solid-phase synthesis is a key parameter for successful peptide synthesis. This chapter provides an overview of the most common and useful resins and linkers for the synthesis of peptides with C-terminal amides, carboxylic acids, and more. The chapter finishes with robust protocols for general solid-phase peptide synthesis, i.e., the standard operations.
IGM, Faculty of Life Sciences, University of Copenhagen, Zealand Pharma, Glostrup, Denmark.
Pernille Tofteng
PT
Shelton
Knud J
KJ
Jensen
D003432:Cross-Linking Reagents / Q000737:chemistry*; D010455:Peptides / Q000138:chemical synthesis* / Q000737:chemistry; D012117:Resins, Synthetic / Q000737:chemistry*; D060327:Solid-Phase Synthesis Techniques
D003432:Cross-Linking Reagents; D010455:Peptides; D012117:Resins, Synthetic