Bacterial strain typing.
Over the course of the past several decades, rapid advancements in molecular technologies have revolutionized the practice of public health microbiology, and have fundamentally changed the nature, accuracy, and timeliness of laboratory data for outbreak investigation and response. Whole-genome sequencing, in particular, is becoming an increasingly feasible and cost-effective approach for near real-time high-resolution strain typing, genomic characterization, and comparative analyses. This review discusses the current state of the art in bacterial strain typing for outbreak investigation and infectious disease surveillance, and the impact of emerging genomic technologies on the field of public health microbiology.
National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road Northeast, MS-C12, Atlanta, GA 30333, USA. dmaccannell@cdc.gov
Duncan
D
MacCannell
D015373:Bacterial Typing Techniques; D016521:Electrophoresis, Gel, Pulsed-Field / Q000379:methods; D016680:Genome, Bacterial; D006801:Humans; D017422:Sequence Analysis, DNA / Q000379:methods
Bacterial typing techniques; DNA sequence analysis; Genomics; Molecular epidemiology; Multilocus sequence typing; Pulsed-field gel electrophoresis