Comparative toxicity of CuO nanoparticles and CuSO4 in rainbow trout.
This study compared the toxicity and accumulation of two different Cu compounds, CuO nanoparticles (NPs) and soluble CuSO4, in erythrocytes and different tissues in rainbow trout (Oncorhynchus mykiss). The crystal structure of CuO NP analysed by XRD indicates that the NP are Tenorite, a monoclinic CuO. The in vitro toxicity results indicate that both Cu compounds increase the haemolysis rate in a dose-dependent way, but the effect was reduced treating cells with CuO NP. Moreover, both Cu compounds induce DNA damage and the entity of the damage, similarly to haemolysis, was more marked in cells treated with CuSO4. In vivo results, obtained after intraperitoneal injection, showed that Cu concentrations were significantly higher in gills (p<0.0001), kidney (p=0.007) and liver (p<0.05) of exposed fish with a significant increase in plasma Cu concentration 15h after CuSO4 treatment. Cu concentrations were significantly higher in fish exposed to CuSO4 than CuO in kidney (p<0.05) and gills (p<0.0001). Significant DNA damage with respect to controls was detected only when Cu was injected as CuSO4. The present data could serve to evaluate environmental Cu toxicity in fish depending on Cu speciation.
Department of Veterinary Medical Sciences, University of Bologna, Italy. Electronic address: gloria.isani@unibo.it.
Gloria
G
Isani
Maria Letizia
ML
Falcioni
Gianni
G
Barucca
Durairaj
D
Sekar
Giulia
G
Andreani
Emilio
E
Carpenè
Giancarlo
G
Falcioni
D000818:Animals; D003300:Copper / Q000633:toxicity*; D019327:Copper Sulfate / Q000633:toxicity*; D004249:DNA Damage / Q000187:drug effects; D004912:Erythrocytes / Q000187:drug effects; D005880:Gills / Q000187:drug effects; D006461:Hemolysis / Q000187:drug effects; D007668:Kidney / Q000187:drug effects; D008099:Liver / Q000187:drug effects; D053758:Nanoparticles / Q000633:toxicity* / Q000648:ultrastructure; D017686:Oncorhynchus mykiss / Q000502:physiology*; D014874:Water Pollutants, Chemical / Q000032:analysis / Q000633:toxicity*
D014874:Water Pollutants, Chemical; D003300:Copper; D019327:Copper Sulfate; C030973:cupric oxide
CuO nanoparticles; CuSO(4); DNA damage; Erythrocytes; Rainbow trout; Tissues