Biochemical changes in erythrocyte membrane in uncontrolled type 2 diabetes mellitus.
The levels of lipid peroxidation and alterations in lipid composition and ATPase activities were determined in erythrocyte plasma membrane of uncontrolled type 2 diabetes mellitus (DM) patients. The study groups consisted of 30 patients (16 males, 14 females) attending the Out Patients' Department of Lokmanya Tilak Municipal General Hospital, Mumbai, and 23 age- and sex-matched control subjects (15 males, 8 females). Glycated haemoglobin (an index of long-term glycaemic control), erythrocyte membrane cholesterol, phospholipid and cholesterol to phospholipid molar ratio, lipid peroxidation products in the form of thiobarbituric acid-reactive substances (TBARS), and Na(+)-K(+)-ATPase activity were found to be significantly increased, and Mg(2+)-ATPase activity significantly decreased, in the diabetic subjects, as compared to controls. The study suggests that biochemical changes in the erythrocyte membrane may be involved in the pathophysiology of type 2 DM.
Department of Biochemistry, Lokmanya Tilak Municipal Medical College and General Hospital, Sion, Mumbai 400022.
Shamlan M S
SM
Reshamwala
Neela D
ND
Patil
D015415:Biomarkers / Q000032:analysis*; D001786:Blood Glucose / Q000378:metabolism; D017301:Ca(2+) Mg(2+)-ATPase / Q000378:metabolism; D016022:Case-Control Studies; D002784:Cholesterol / Q000378:metabolism; D003924:Diabetes Mellitus, Type 2 / Q000503:physiopathology*; D004910:Erythrocyte Membrane / Q000378:metabolism* / Q000473:pathology; D005260:Female; D006442:Glycated Hemoglobin / Q000378:metabolism; D006801:Humans; D015227:Lipid Peroxidation; D008297:Male; D008875:Middle Aged; D010743:Phospholipids / Q000378:metabolism; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
D015415:Biomarkers; D001786:Blood Glucose; D017301:Ca(2+) Mg(2+)-ATPase; D002784:Cholesterol; D006442:Glycated Hemoglobin; D010743:Phospholipids; D000254:Sodium-Potassium-Exchanging ATPase; D017392:Thiobarbituric Acid Reactive Substances