A highly sensitive europium nanoparticle-based lateral flow immunoassay for detection of chloramphenicol residue.
A europium nanoparticle-based lateral flow immunoassay for highly sensitive detection of chloramphenicol residue was developed. The detection result could be either qualitatively resolved with naked eye or quantitatively analyzed with the assistance of a digital camera. In the qualitative mode, the limit of detection (LOD) was found to be 0.25 ng/mL. In the quantitative mode, the half-maximal inhibition concentration (IC50) was determined to be 0.45 ng/mL and the LOD can reach an ultralow level of 0.03 ng/mL, which is ~100 times lower than that of the conventional colloidal gold-based lateral flow immunoassay. Potential application of the established method was demonstrated by analyzing representative cow milk samples.
Engineering Research Centre of Molecular Diagnostics, Ministry of Education, Department of Biomedical Sciences, School of Life Sciences, Xiamen University, Xiamen, Fujian, 361005, China.
Xiaohu
X
Xia
Ye
Y
Xu
Rongqin
R
Ke
Heng
H
Zhang
Mingqiang
M
Zou
Wei
W
Yang
Qingge
Q
Li
D000818:Animals; D000906:Antibodies / Q000737:chemistry / Q000302:isolation & purification; D002701:Chloramphenicol / Q000032:analysis*; D005063:Europium / Q000737:chemistry; D006433:Hemocyanins / Q000737:chemistry; D007118:Immunoassay; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry*; D008892:Milk / Q000737:chemistry*; D011817:Rabbits
D000906:Antibodies; D005063:Europium; D002701:Chloramphenicol; D006433:Hemocyanins; C032808:keyhole-limpet hemocyanin