Easy upgrade of the TRACERLab FX C Pro for [¹¹C]carboxylation reactions: application to the routine production of [1-¹¹C]acetate.
Carbon-11-labeled acetate ([1-(11)C]acetate) is a radiopharmaceutical of importance in clinical practice as well as in preclinical research in cardiology and oncology. Its preparation is based on the [(11)C]carboxylation reaction of a Grignard reagent with [(11)C]CO2. Most of the commercially available synthesizers are only dedicated to the preparation of [(11)C]methyl iodide (or [(11)C]methyl triflate) for the radiomethylation of an appropriate precursor but not for the direct use of cyclotron-produced [(11)C]CO2. Based on the classical [(11)C]carboxylation reaction and SPE purification, we propose in this technical note a detailed, simple, easy-to-handle and fully reversible modification of the TRACERLab FX C Pro to operate, on demand, [(11)C]carboxylation reactions, exemplified herein by the production of [1-(11)C]acetate, or [(11)C]radiomethylation reactions. This also opens new prospects to other type of radiochemical reactions involving [(11)C]CO2.
CEA, I²BM, Service Hospitalier Frédéric Joliot, Orsay, France.
Stéphane
S
Le Helleix
Frédéric
F
Dollé
Bertrand
B
Kuhnast
D000085:Acetates / Q000138:chemical synthesis*; D002244:Carbon; D002245:Carbon Dioxide; D002250:Carbon Radioisotopes; D002851:Chromatography, High Pressure Liquid; D004867:Equipment Design; D006801:Humans; D011854:Radiochemistry / Q000295:instrumentation*; D019275:Radiopharmaceuticals / Q000138:chemical synthesis*
D000085:Acetates; D002250:Carbon Radioisotopes; D019275:Radiopharmaceuticals; C438206:carbon-11 acetate; D002245:Carbon Dioxide; D002244:Carbon
Carboxylation; PET; Positron emission tomography; TRACERLab FX C Pro; [1-(11)C]acetate