Antibacterial activity of the recombinant antimicrobial peptide Ib-AMP4 from Impatiens balsamina and its synergy with other antimicrobial agents against drug resistant bacteria.
Ib-AMP4 is an antimicrobial peptide of Impatiens balsamina (Balsaminaceae). Ib-AMP4 was produced as a recombinant peptide and in this study its antimicrobial activity against human bacterial pathogens was investigated. Ib-AMP4 was bactericidal against both Gram positive and Gram negative bacteria with MIC values between 0.49 and 3.5 microM in sensitive species. A genuine synergistic effect was achieved when IB-AMP4 was employed in combination with the plant monoterpene thymol against drug-resistant Klebsiella pneumoniae (KPC) ATCC700603, or with the antibiotics vancomycin or oxacillin against Enterococcus faecalis (VRE) ATCC51299.
Institut für Pharmazie und Molekulare Biotechnologie, Universitit Heidelberg, Germany.
X
X
Fan
J
J
Reichling
M
M
Wink
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D023181:Antimicrobial Cationic Peptides / Q000378:metabolism* / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects; D003470:Culture Media; D024881:Drug Resistance, Bacterial; D004357:Drug Synergism; D005347:Fibroblasts / Q000187:drug effects; D006094:Gram-Positive Bacteria / Q000187:drug effects; D029069:Impatiens / Q000378:metabolism*; D007700:Kinetics; D055624:Methicillin-Resistant Staphylococcus aureus / Q000187:drug effects; D008826:Microbial Sensitivity Tests
D000900:Anti-Bacterial Agents; D023181:Antimicrobial Cationic Peptides; D003470:Culture Media; C504508:Ib-AMP4 peptide, Impatiens balsamina