Online correction of licking-induced brain motion during two-photon imaging with a tunable lens.
Two-photon calcium imaging in awake, head-fixed animals enables the measurement of neuronal activity during behaviour. Often, licking for the retrieval of water reward is used as a measurable report of the animal's decision during reward-driven behaviour. However, licking behaviour can induce severe motion artifacts that interfere with two-photon imaging of cellular activity. Here, we describe a simple method for the online correction of licking-induced focus shifts for two-photon calcium imaging of neocortical neurons in the head-fixed mouse. We found that licking causes a stereotyped drop of neocortical tissue, shifting neurons up to 20 μm out of focus. Based on the measurement of licking with a piezo film sensor, we developed a feedback model, which provides a corrective signal for fast optical focus adjustments with an electrically tunable lens. Using online correction with this feedback model, we demonstrate a reduction of licking-related focus changes below 3 μm, minimizing motion artifact contamination of cellular calcium signals. Focus correction with a tunable lens is a simple and effective method to improve the ability to monitor neuronal activity during reward-based behaviour.
J. L. Chen: University of Zurich, Brain Research Institute, Winterthurerstrasse 190, 55H64, Zurich, CH-8057, Switzerland. jerry.chen@hifo.uzh.ch.
Jerry L
JL
Chen
Oliver A
OA
Pfäffli
Fabian F
FF
Voigt
David J
DJ
Margolis
Fritjof
F
Helmchen
D000818:Animals; D016477:Artifacts; D001522:Behavior, Animal; D020013:Calcium Signaling; D007091:Image Processing, Computer-Assisted; D051379:Mice; D008810:Mice, Inbred C57BL; D036641:Microscopy, Fluorescence, Multiphoton / Q000379:methods*; D009038:Motion; D019579:Neocortex / Q000166:cytology / Q000502:physiology*; D009474:Neurons / Q000378:metabolism / Q000502:physiology; D061848:Optical Imaging / Q000379:methods; D012201:Reward