Co-expression pattern of dopamine beta-hydroxylase (DβH) and neuropeptide Y (NPY) within sympathetic innervation of ovary and umbilical cord of the European bison (Bison bonasus L.).
Co-expression of dopamine β-hydroxylase (DβH) and neuropeptide Y (NPY) has never been examined in ovary (OV) and umbilical cord (UC) of the European bison (Eb), the endangered wild species. The OV and UC samples were harvested from seasonally eliminated Eb females (45-120 days post coitum). Frozen histological sections were examined by double fluorescent immunohistochemistry (dF-IHC), using the primary mouse anti-DβH monoclonals and rabbit anti-NPY polyclonals and then the immunocomplexes were visualized with FITC and CY3 fluorophores, respectively. Numerous DβH immunoreactive nerve fibers (DβH-IRs) and a little less frequent NPY-IRs were found in the bundle-like structures, innervating mainly perivascular regions of the OV. The NPY-IRs constantly co-expressed DβH, while some DβH-IRs did not express NPY. This specific pattern of innervation was observed both in the stromal and cortical regions of the OV. The simultaneous co-expression of DβH and NPY were also detected in the UC, in which specific single or bundle-like structures ran along the smooth muscles of blood vessels. The spatial-specific co-expression of DβH and NPY in OV and UC, may suggest that these markers are involved in the control of vascularization that regulates nourishing blood circulation required for proper pregnancy maintenance and efficient embryo/fetus development in the Eb.
Department of Pathology and Laboratory Medicine University of Tennessee Health Science Center, Memphis, TN 38163, USA.
Cezary
C
Skobowiat
Grzegorz
G
Panasiewicz
Zygmunt
Z
Gizejewski
Bozena
B
Szafranska
D000818:Animals; D016164:Bison; D001775:Blood Circulation; D004299:Dopamine beta-Hydroxylase / Q000096:biosynthesis*; D004622:Embryo, Mammalian; D047108:Embryonic Development / Q000235:genetics; D005260:Female; D018919:Neovascularization, Physiologic / Q000235:genetics*; D009412:Nerve Fibers / Q000502:physiology; D009478:Neuropeptide Y / Q000096:biosynthesis*; D010053:Ovary / Q000294:innervation*; D014470:Umbilical Cord / Q000294:innervation*
D009478:Neuropeptide Y; D004299:Dopamine beta-Hydroxylase
Bison bonasus L.; DβH; Eb; NPY; Ovary; Umbilical cord