Systematic analysis and comparison of nucleotide-binding site disease resistance genes in a diploid cotton Gossypium raimondii.
Plant disease resistance genes are a key component of defending plants from a range of pathogens. The majority of these resistance genes belong to the super-family that harbors a Nucleotide-binding site (NBS). A number of studies have focused on NBS-encoding genes in disease resistant breeding programs for diverse plants. However, little information has been reported with an emphasis on systematic analysis and comparison of NBS-encoding genes in cotton. To fill this gap of knowledge, in this study, we identified and investigated the NBS-encoding resistance genes in cotton using the whole genome sequence information of Gossypium raimondii. Totally, 355 NBS-encoding resistance genes were identified. Analyses of the conserved motifs and structural diversity showed that the most two distinct features for these genes are the high proportion of non-regular NBS genes and the high diversity of N-termini domains. Analyses of the physical locations and duplications of NBS-encoding genes showed that gene duplication of disease resistance genes could play an important role in cotton by leading to an increase in the functional diversity of the cotton NBS-encoding genes. Analyses of phylogenetic comparisons indicated that, in cotton, the NBS-encoding genes with TIR domain not only have their own evolution pattern different from those of genes without TIR domain, but also have their own species-specific pattern that differs from those of TIR genes in other plants. Analyses of the correlation between disease resistance QTL and NBS-encoding resistance genes showed that there could be more than half of the disease resistance QTL associated to the NBS-encoding genes in cotton, which agrees with previous studies establishing that more than half of plant resistance genes are NBS-encoding genes.
Key Laboratory of Crop Germplasm, Department of Agronomy, Zhejiang University, Hangzhou, Zhejiang, China.
Hengling
H
Wei
Wei
W
Li
Xiwei
X
Sun
Shuijin
S
Zhu
Jun
J
Zhu
D001665:Binding Sites; D004171:Diploidy; D060467:Disease Resistance / Q000235:genetics*; D019143:Evolution, Molecular; D020440:Gene Duplication; D017343:Genes, Plant / Q000235:genetics*; D023281:Genomics; D003368:Gossypium / Q000235:genetics* / Q000276:immunology* / Q000382:microbiology; D009711:Nucleotides / Q000378:metabolism*; D010802:Phylogeny; D010935:Plant Diseases / Q000382:microbiology; D040641:Quantitative Trait Loci / Q000235:genetics
D009711:Nucleotides