Age-related changes in the elemental constituents and molecular behaviour of bone.
Age-related changes in bone composition within the age groups of 30, 60, 120 and 180 days in rats have been studied using thermogravimetery, elemental analysis and energy dispersive Xray (ED X-ray). The structural changes in rats the bone samples were monitored by IR spectroscopy. The main constituents of hydroxyapatite, namely Ca, P and their oxides were analyzed. Organic changes, such as C and N contents in the matrix were found to have a predominant role in the initial development of the bone. An incremental increase in the mineral content of bone with advancing age was also observed. Elemental composition (C and N contents) was observed to be independent of age at the initial stages. The amount of Ca and its oxide content was found to increase, and the P and its oxides showed a decreasing trend, with the advancing age. IR spectra revealed that the mineral phase comprised both amorphous and crystalline hydroxyapatite, even at maturity; the amorphous content being higher at the earlier stages (14.09%, at 30 days), but was gradually replaced by crystalline component with advancing age (63.09% at 180 days). The present data may be useful in explaining the ageing phenomenon and helpful in understanding the bone growth and remodeling.
Department of Biophysics, Panjab University, Chandigarh 160 014, India. dvrai@pu.ac.in
D V
DV
Rai
R
R
Darbari
L M
LM
Aggarwal
D000375:Aging / Q000502:physiology*; D000818:Animals; D015519:Bone Density / Q000502:physiology*; D001846:Bone Development / Q000502:physiology*; D001842:Bone and Bones / Q000737:chemistry*; D002118:Calcium / Q000032:analysis; D017886:Durapatite / Q000032:analysis; D004602:Elements; D008297:Male; D010087:Oxides / Q000032:analysis; D010710:Phosphates / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar; D013055:Spectrophotometry, Infrared; D014961:X-Ray Diffraction
D004602:Elements; D010087:Oxides; D010710:Phosphates; D017886:Durapatite; D002118:Calcium