Scale-up of the process to obtain functional ingredients based in plasma protein concentrates from porcine blood.
The feasibility of a scaled-up process to obtain two protein concentrates from porcine blood plasma, i.e. serum and albumin, for use as functional food ingredients was assessed. The process consisted of fractionating plasma proteins by salting out, concentrating and purifying fractions by means of membrane technology, and subsequently dehydrating through spray-drying. The fractionation process allowed a good isolation of the desired proteins, which were then concentrated and desalted in a tangential flow filtration (TFF) process combining ultra and diafiltration. Purification, pre-concentration and dehydration were successfully achieved. The functional properties of dehydrated serum and albumin were determined. As compared to the same hemoderivatives obtained by a lab-scale production system, serum maintained the gelling properties; albumin exhibited similar foaming properties; and both serum and albumin concentrates showed slightly improved emulsifying properties.
Institut de Tecnologia Agroalimentària (INTEA), XaRTA, Universitat de Girona, Escola Politècnica Superior, C/Maria Aurèlia Capmany 61, 17071 Girona, Spain.
Dolors
D
Parés
Mònica
M
Toldrà
Elena
E
Saguer
Carmen
C
Carretero
D000818:Animals; D001798:Blood Proteins / Q000737:chemistry*; D003890:Desiccation; D004591:Electrophoresis, Polyacrylamide Gel; D045703:Emulsifying Agents / Q000737:chemistry; D004655:Emulsions / Q000737:chemistry; D005240:Feasibility Studies; D005374:Filtration / Q000379:methods; D005503:Food Additives / Q000737:chemistry*; D005511:Food Handling / Q000379:methods*; D005782:Gels / Q000737:chemistry; D010949:Plasma / Q000737:chemistry*; D012492:Salts / Q000032:analysis; D012709:Serum Albumin / Q000032:analysis; D013552:Swine
D001798:Blood Proteins; D045703:Emulsifying Agents; D004655:Emulsions; D005503:Food Additives; D005782:Gels; D012492:Salts; D012709:Serum Albumin
Albumin; Functional properties; Salting out; Serum; Tangential flow filtration