Isolation and enrichment of pathogens with a surface-modified aluminium chip for Raman spectroscopic applications.
We developed a Raman-compatible chip for isolating microorganisms from complex media. The isolation of bacteria is achieved by using antibodies as capture molecules. Due to the very specific interaction with the targets, this approach is promising for isolation of bacteria even from complex matrices such as body fluids. Our choice of capture molecules also enabled the investigation of samples containing yet unidentified bacteria, as the antibodies can capture a large variety of bacteria based on their analogue cell wall surface structures. The capability of our system is demonstrated for a broad range of different Gram-positive and Gram-negative germs. Subsequent identification is done by recording Raman spectra of the bacteria. Further, it is shown that classification with chemometric methods is possible.
Spectroscopy and Imaging, Institute of Photonic Technology, Albert-Einstein-Str. 9, 07745 Jena (Germany), Fax: (+49) (0)3641 206 399; Institute of Physical Chemistry, Friedrich-Schiller-University Jena, Helmholtzweg 4, 07743 Jena (Germany).
Susanne
S
Pahlow
Sandra
S
Kloß
Verena
V
Blättel
Konstantin
K
Kirsch
Uwe
U
Hübner
Dana
D
Cialla
Petra
P
Rösch
Karina
K
Weber
Jürgen
J
Popp
D000535:Aluminum / Q000737:chemistry*; D000906:Antibodies / Q000276:immunology; D015374:Biosensing Techniques; D016002:Discriminant Analysis; D006090:Gram-Negative Bacteria / Q000276:immunology / Q000302:isolation & purification*; D006094:Gram-Positive Bacteria / Q000276:immunology / Q000302:isolation & purification*; D008828:Microbiological Techniques / Q000379:methods*; D025341:Principal Component Analysis; D012821:Silanes / Q000737:chemistry; D013059:Spectrum Analysis, Raman; D013499:Surface Properties
D000906:Antibodies; D012821:Silanes; D000535:Aluminum
Raman spectroscopy; aluminium; antibodies; biosensors; silanes