Preliminary results of the application of gonadal tissue transfer in various chicken breeds in the poultry gene conservation.
The aim of the study was to devise a technique to allow transfer of ovarian and testicular tissues obtained from one day old chicks to recipient animals. The following combinations of chicken breeds were used: Tetra SL/Tetra SL, Tetra SL/Harco, Tetra SL/Black Transylvanian Naked Neck, Godollo New Hampshire/Speckled Transylvanian Naked Neck (recipient/donor), respectively. Only animals less than 24h old from hatching were used as either recipient or donor. Eggs yielding recipients were treated with busulfan to hinder the development of the recipient's own gonads. Gonads were transferred surgically to the new host which was then immunosuppressed for two months. The animals were checked at 8 or 16 weeks for the existence of implanted gonads, and if found, the gonads were removed for histology examination. Tetra SL/Tetra SL pairing resulted in successfully adhered and functioning gonads in most cases. In other combinations, no gonads originating from the donors were found in the recipients. We conclude that not all breeds seem to be suitable recipients; some breeds or individuals may show incompatibility to each other and further examination is needed to find the cause of incompatibility and to establish a suitable breed combination, which can be used for gonad transfer.
Institute for Small Animal Research and Co-ordination Centre for Gene Conservation, Isaszegi u. 200, H-2100 Godollo, Hungary. liptoi@katki.hu
Krisztina
K
Liptoi
Gabriella
G
Horvath
Janos
J
Gal
Eva
E
Varadi
Judit
J
Barna
D000818:Animals; D000831:Animals, Newborn; D002645:Chickens / Q000235:genetics* / Q000502:physiology*; D005260:Female; D008297:Male; D010053:Ovary / Q000502:physiology*; D013737:Testis / Q000502:physiology*; D014021:Tissue Preservation / Q000662:veterinary*; D016378:Tissue Transplantation / Q000379:methods / Q000662:veterinary*
Gene conservation; Gonadal grafting; Poultry