Binding mechanism of the peptidoglycan hydrolase Acm2: low affinity, broad specificity.
Peptidoglycan hydrolases are bacterial secreted enzymes that cleave covalent bonds in the cell-wall peptidoglycan, thereby fulfilling major physiological functions during cell growth and division. Although the molecular structure and functional roles of these enzymes have been widely studied, the molecular details underlying their interaction with peptidoglycans remain largely unknown, mainly owing to the paucity of appropriate probing techniques. Here, we use atomic force microscopy to explore the binding mechanism of the major autolysin Acm2 from the probiotic bacterium Lactobacillus plantarum. Atomic force microscopy imaging shows that incubation of bacterial cells with Acm2 leads to major alterations of the cell-surface nanostructure, leading eventually to cell lysis. Single-molecule force spectroscopy demonstrates that the enzyme binds with low affinity to structurally different peptidoglycans and to chitin, and that glucosamine in the glycan chains is the minimal binding motif. We also find that Acm2 recognizes mucin, the main extracellular component of the intestinal mucosal layer, thereby suggesting that this enzyme may also function as a cell adhesion molecule. The binding mechanism (low affinity and broad specificity) of Acm2 may represent a generic mechanism among cell-wall hydrolases for guiding cell division and cell adhesion.
Université catholique de Louvain, Institute of Life Sciences, Croix du Sud, Louvain-la-Neuve, Belgium.
Audrey
A
Beaussart
Thomas
T
Rolain
Marie-Clémence
MC
Duchêne
Sofiane
S
El-Kirat-Chatel
Guillaume
G
Andre
Pascal
P
Hols
Yves F
YF
Dufrêne
D020816:Amino Acid Motifs; D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism*; D001665:Binding Sites; D002462:Cell Membrane / Q000378:metabolism / Q000648:ultrastructure; D002686:Chitin / Q000378:metabolism; D005944:Glucosamine / Q000378:metabolism; D048191:Lactiplantibacillus plantarum / Q000201:enzymology*; D018625:Microscopy, Atomic Force; D009238:N-Acetylmuramoyl-L-alanine Amidase / Q000737:chemistry / Q000378:metabolism*; D011135:Polysaccharides, Bacterial / Q000378:metabolism; D011485:Protein Binding; D013379:Substrate Specificity
D001426:Bacterial Proteins; D011135:Polysaccharides, Bacterial; D002686:Chitin; D009238:N-Acetylmuramoyl-L-alanine Amidase; D005944:Glucosamine