Preimplantation genetic screening (PGS) with Comparative genomic hybridization (CGH) following day 3 single cell blastomere biopsy markedly improves IVF outcomes while lowering multiple pregnancies and miscarriages.
OBJECTIVE
To determine benefits of cleavage-stage preimplantation genetic screening (PGS) by array comparative genomic hybridization (CGH).


METHODS
A retrospective case-control study was performed at a tertiary care university-affiliated medical center. Implantation rate was looked at as a primary outcome. Secondary outcomes included clinical and ongoing pregnancy rates, as well as multiple pregnancy and miscarriage rates. Thirty five patients underwent 39 fresh cycles with PGS by aCGH and 311 similar patients underwent 394 invitro fertilization cycles.


RESULTS
The implantation rate in the CGH group doubled when compared to the control group (52.63 % vs. 19.15 %, p = < 0.001), clinical pregnancy rate was higher (69.23 % vs. 43.91 %, p = 0.0002), ongoing pregnancy rate almost doubled (61.54 % vs. 32.49 %, p = < 0.0001), multiple pregnancy rate decreased (8.33 % vs. 34.38 %, p = 0.0082) and miscarriage rate trended lower (11.11 % vs. 26.01 %, p = 0.13).


CONCLUSIONS
Cleavage stage PGS with CGH is a feasible and safe option for aneuploidy screening that shows excellent outcomes when used in fresh cycles. This is the first report of cleavage stage PGS by CGH showing improved ongoing pregnancy rates.
Continuum Reproductive Center, Department of Obstetrics and Gynecology, St. Luke's-Roosevelt Hospital Center, Columbia University, College of Physicians and Surgeons, New York, NY, USA, mdkeltz@aol.com.
Martin D
MD
Keltz
Mario
M
Vega
Ido
I
Sirota
Matthew
M
Lederman
Erin L
EL
Moshier
Eric
E
Gonzales
Daniel
D
Stein
D000022:Abortion, Spontaneous; D000328:Adult; D001757:Blastomeres / Q000166:cytology; D016022:Case-Control Studies; D055028:Comparative Genomic Hybridization; D010064:Embryo Implantation; D004624:Embryo Transfer; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods*; D006801:Humans; D050498:Live Birth; D011247:Pregnancy; D018873:Pregnancy Rate; D011272:Pregnancy, Multiple; D019836:Preimplantation Diagnosis; D012189:Retrospective Studies