Multiclass method for antimicrobial analysis in animal feeds by liquid chromatography-tandem mass spectrometry.
A rapid multiclass method that covers 50 antimicrobials from 13 different families in animal feeds was developed. Samples were extracted using a mixture of methanol, acetonitrile and a McIlvaine buffer combined with sonication. Feed extracts were simply diluted prior to injection, since the clean-up strategies that were tested, based on either solid-phase extraction or dispersive solid-phase extraction, were ineffective at minimizing matrix-related signal suppression/enhancement. Analysis was carried out by liquid chromatography coupled to tandem mass spectrometry using an electrospray ionization source operating in positive and negative modes. For the quantification, matrix-fortified standard calibration curves were used to compensate for matrix effects and losses in sample preparation. The method was validated in-house in pig, poultry and cattle feed matrices and showed satisfactory performance characteristics. Thus, the proposed approach was suitable for application in a routine high-throughput laboratory for the official control of feeds.
Departament de Química Analítica, Universitat de Barcelona, 08028, Barcelona, Spain.
S
S
Borràs
R
R
Companyó
J
J
Guiteras
J
J
Bosch
M
M
Medina
S
S
Termes
D000821:Animal Feed / Q000032:analysis*; D000818:Animals; D000890:Anti-Infective Agents / Q000032:analysis*; D002417:Cattle; D002853:Chromatography, Liquid / Q000379:methods*; D057230:Limit of Detection; D011200:Poultry; D013552:Swine; D053719:Tandem Mass Spectrometry / Q000379:methods*
D000890:Anti-Infective Agents