An integrative model of the control of ovule primordia formation.
Upon hormonal signaling, ovules develop as lateral organs from the placenta. Ovule numbers ultimately determine the number of seeds that develop, and thereby contribute to the final seed yield in crop plants. We demonstrate here that CUP-SHAPED COTYLEDON 1 (CUC1), CUC2 and AINTEGUMENTA (ANT) have additive effects on ovule primordia formation. We show that expression of the CUC1 and CUC2 genes is required to redundantly regulate expression of PINFORMED1 (PIN1), which in turn is required for ovule primordia formation. Furthermore, our results suggest that the auxin response factor MONOPTEROS (MP/ARF5) may directly bind ANT, CUC1 and CUC2 and promote their transcription. Based on our findings, we propose an integrative model to describe the molecular mechanisms of the early stages of ovule development.
Dipartimento di Bioscienze, Università degli Studi di Milano, Via Celoria 26, 20133, Milano, Italy.
Francesca
F
Galbiati
Dola
D
Sinha Roy
Sara
S
Simonini
Mara
M
Cucinotta
Luca
L
Ceccato
Candela
C
Cuesta
Maria
M
Simaskova
Eva
E
Benkova
Yuri
Y
Kamiuchi
Mitsuhiro
M
Aida
Dolf
D
Weijers
Rüdiger
R
Simon
Simona
S
Masiero
Lucia
L
Colombo
D017360:Arabidopsis / Q000196:embryology* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000378:metabolism / Q000502:physiology*; D004268:DNA-Binding Proteins / Q000378:metabolism; D049452:Green Fluorescent Proteins; D026901:Membrane Transport Proteins / Q000378:metabolism; D008954:Models, Biological; D056771:Ovule / Q000196:embryology*; D011401:Promoter Regions, Genetic; D014157:Transcription Factors / Q000378:metabolism / Q000502:physiology*
C099055:AINTEGUMENTA protein, Arabidopsis; D029681:Arabidopsis Proteins; C426007:CUC1 protein, Arabidopsis; C107294:CUC2 protein, Arabidopsis; D004268:DNA-Binding Proteins; C111371:MONOPTEROS protein, Arabidopsis; D026901:Membrane Transport Proteins; C116363:PIN1 protein, Arabidopsis; D014157:Transcription Factors; D049452:Green Fluorescent Proteins
AINTEGUMENTA; Arabidopsis; CUP-SHAPED COTYLEDON; MONOPTEROS; PIN1; ovule development