The secretome of Trametes versicolor grown on tomato juice medium and purification of the secreted oxidoreductases including a versatile peroxidase.
The present work was carried out with the aim to analyze the secretome of Trametes versicolor BAFC 2234 grown on tomato juice medium supplemented with copper and manganese. T. versicolor BAFC 2234 was selected among diverse wood dwelling agaricomycetes from Argentina by its ability to cause a strong white rot on hardwood and in addition to show high tolerance toward phenolic compounds. A considerable number of the identified proteins were related to the degradation/modification of lignocelluloses. Hydrolases, peroxidases and phenoloxidases were the most abundant enzymes produced under the above-mentioned culture conditions. The lignin-modifying oxidoreductases laccase, manganese peroxidase (MnP) and versatile peroxidase (VP) were successfully purified - the latter for the first time from T. versicolor. The native VP protein has a molecular mass of 45kDa and an isoelectric point of pH 3.7. The study clearly shows that complex plant-based media being rich in phenolics, such as tomato juice, can stimulate the secretion of a broad set of extracellular lignocellulolytic enzymes. Using such natural products as fungal culture media may give the opportunity to investigate plant biomass decomposition as well as the biodegradation of organic pollutants in an environment close to nature.
TU Dresden, International Institute Zittau, Department of Bio- and Environmental Sciences, Markt 23, 02763 Zittau, Germany; University of Buenos Aires, Faculty of Exact and Natural Sciences, Department of Biodiversity and Experimental Biology, Intendente Güiraldes 2160, Ciudad Universitaria, C1428EGA, Argentina. Electronic address: mcarabajal@conicet.gov.ar.
Maira
M
Carabajal
Harald
H
Kellner
Laura
L
Levin
Nico
N
Jehmlich
Martin
M
Hofrichter
René
R
Ullrich
D003470:Culture Media / Q000494:pharmacology; D006867:Hydrolases / Q000378:metabolism; D018551:Solanum lycopersicum / Q000737:chemistry*; D014442:Monophenol Monooxygenase / Q000378:metabolism; D010088:Oxidoreductases / Q000378:metabolism*; D009195:Peroxidase / Q000378:metabolism*; D055454:Trametes / Q000187:drug effects / Q000201:enzymology*
D003470:Culture Media; D010088:Oxidoreductases; D009195:Peroxidase; D014442:Monophenol Monooxygenase; D006867:Hydrolases
Laccase; Manganese peroxidase; Secretome; Trametes versicolor; Versatile peroxidase