Hydrocarbon biodegradation and dynamic laser speckle for detecting chemotactic responses at low bacterial concentration.
We report on the biodegradation of pure hydrocarbons and chemotaxis towards these compounds by an isolated chlorophenol degrader, Pseudomonas strain H. The biochemical and phylogenetic analysis of the 16S rDNA sequence identified Pseudomonas strain H as having 99.56% similarity with P. aeruginosa PA01. This strain was able to degrade n-hexadecane, 1-undecene, 1-nonene, 1-decene, 1-dodecene and kerosene. It grew in the presence of 1-octene, while this hydrocarbons is toxic to other hydrocarbons degraders. Pseudomonas strain H was also chemotactic towards n-hexadecane, kerosene, 1-undecene and 1-dodecene. These results show that this Pseudomonas strain H is an attractive candidate for hydrocarbon-containing wastewater bioremediation in controlled environments. Since the classical standard techniques for detecting chemotaxis are not efficient at low bacterial concentrations, we demonstrate the use of the dynamic speckle laser method, which is simple and inexpensive, to confirm bacterial chemotaxis at low cell concentrations (less than 10(5) colony-forming unit per millilitre (CFU/mL)) when hydrocarbons are the attractants.
National University of Mar del Plata, Faculty of Engineering, Department of Chemistry, Biochemical Engineering Group, Juan B. Justo 4302, (7600), Mar del Plata, Argentina. melinanisenbaum@gmail.com
Melina
M
Nisenbaum
Gonzalo Hernán
GH
Sendra
Gastón Alfredo Cerdá
GA
Gilbert
Marcelo
M
Scagliola
Jorge Froilán
JF
González
Silvia Elena
SE
Murialdo
D001673:Biodegradation, Environmental / Q000187:drug effects; D002244:Carbon / Q000494:pharmacology; D002633:Chemotaxis / Q000187:drug effects*; D005110:Extracellular Space / Q000737:chemistry; D006838:Hydrocarbons / Q000494:pharmacology*; D007834:Lasers; D011549:Pseudomonas / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism* / Q000648:ultrastructure; D013501:Surface-Active Agents / Q000494:pharmacology
D006838:Hydrocarbons; D013501:Surface-Active Agents; D002244:Carbon