Recombinant flagellin and its cross-talk with lipopolysaccharide--effect on pooled chicken peripheral blood mononuclear cells.
Toll-like receptors (TLRs) are one of the types of pattern recognition receptors (PRRs) that recognize conserved pathogen molecules. TLRs link innate and adaptive arms of immune system and are implicated in the development of defense against invading pathogens. Lipopolysaccharide (LPS) and flagellin are recognized by TLR4 and TLR5, respectively. In this study, the effect of flagellin and lipopolysaccharide alone and in combination on chicken peripheral blood mononuclear cells (PBMCs) was investigated. The FliC gene of Salmonella typhimurium was expressed in a prokaryotic expression system and the recombinant flagellin was used to stimulate the chicken PBMCs. A combination of recombinant flagellin and LPS synergistically upregulated nitric oxide production, IL-12 and IL-6 expression but antagonistically down regulated IL-4 expression in comparison to recombinant flagellin alone. The results indicate that these agonists synergistically interact and enhance macrophage function and promote Th1 immune response in chicken PBMCs.
Recombinant DNA Laboratory, Division of Veterinary Biotechnology, Indian Veterinary Research Institute, Izatnagar, Bareilly 243 122, Uttar Pradesh, India. Electronic address: shishirgupta.biotech@gmail.com.
Shishir Kumar
SK
Gupta
Rajib
R
Deb
Satish
S
Gaikwad
R
R
Saravanan
C Madhan
CM
Mohan
Sohini
S
Dey
D000818:Animals; D002645:Chickens; D005408:Flagellin / Q000276:immunology*; D018664:Interleukin-12 / Q000378:metabolism; D015847:Interleukin-4 / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000276:immunology*; D008070:Lipopolysaccharides / Q000276:immunology*; D009569:Nitric Oxide / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary; D020239:Receptor Cross-Talk / Q000276:immunology; D011994:Recombinant Proteins / Q000276:immunology / Q000378:metabolism; D012486:Salmonella typhimurium / Q000276:immunology
D015850:Interleukin-6; D008070:Lipopolysaccharides; D011994:Recombinant Proteins; D005408:Flagellin; D018664:Interleukin-12; D015847:Interleukin-4; D009569:Nitric Oxide
Adjuvant; Agonist; Antagonism; Chicken PBMCs; Cross talk; Cytokine; Flagellin; Lipopolysaccharide; Synergism; Toll-like receptor