A suspension-cell biosensor for real-time determination of binding kinetics of protein-carbohydrate interactions on cancer cell surfaces.
A novel lectin-based suspension-cell biosensor for label-free determination of binding kinetics of protein-carbohydrate interactions on cancer cell surfaces using QCM is described. This cell-biosensor facilitates evaluation of glycosylation in real time on suspension cancer cell surfaces, where binding events take place, more closely mimicking a native environment compared with traditional biosensors.
State Key Laboratory of Crop Stress Biology in Arid Areas and College of Science, Northwest A&F University, Yangling, Shaanxi 712100, People's Republic of China. peizc@nwafu.edu.cn peiyx@nwafu.edu.cn.
Xueming
X
Li
Yuxin
Y
Pei
Ruina
R
Zhang
Qi
Q
Shuai
Feng
F
Wang
Teodor
T
Aastrup
Zhichao
Z
Pei
D015374:Biosensing Techniques; D002241:Carbohydrates / Q000737:chemistry*; D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000737:chemistry*; D006801:Humans; D020014:K562 Cells; D007700:Kinetics; D061848:Optical Imaging; D011485:Protein Binding; D011506:Proteins / Q000737:chemistry*; D013535:Suspensions
D002241:Carbohydrates; D011506:Proteins; D013535:Suspensions