Cell growth and accumulation of polyhydroxyalkanoates from CO2 and H2 of a hydrogen-oxidizing bacterium, Cupriavidus eutrophus B-10646.
Synthesis of polyhydroxyalkanoates (PHAs) by a new strain of Cupriavidus - Cupriavidus eutrophus B-10646 - was investigated under autotrophic growth conditions. Under chemostat, at the specific flow rate D=0.1h(-1), on sole carbon substrate (CO2), with nitrogen, sulfur, phosphorus, potassium, and manganese used as growth limiting elements, the highest poly(3-hydroxybutyrate) [P(3HB)] yields were obtained under nitrogen deficiency. In batch autotrophic culture, in the fermenter with oxygen mass transfer coefficient 0.460 h(-1), P(3HB) yields reached 85% of dry cell weight (DCW) and DCW reached 50 g/l. Concentrations of supplementary PHA precursor substrates (valerate, hexanoate, γ-butyrolactone) and culture conditions were varied to produce, for the first time under autotrophic growth conditions, PHA ter- and tetra-polymers with widely varying major fractions of 3-hydroxybutyrate, 4-hydroxybutyrate, 3-hydroxyvalerate, and 3-hydroxyhexanoate monomer units. Investigation of the high-purity PHA specimens showed significant differences in their physicochemical and physicomechanical properties.
Institute of Biophysics of Siberian Branch of Russian Academy of Sciences, Krasnoyarsk, Russian Federation; Siberian Federal University, Krasnoyarsk, Russian Federation. Electronic address: volova45@mail.ru.
Tatiana G
TG
Volova
Institute of Biophysics of Siberian Branch of Russian Academy of Sciences, Krasnoyarsk, Russian Federation.
Evgeniy G
EG
Kiselev
Institute of Biophysics of Siberian Branch of Russian Academy of Sciences, Krasnoyarsk, Russian Federation; Siberian Federal University, Krasnoyarsk, Russian Federation.
Ekaterina I
EI
Shishatskaya
Institute of Biophysics of Siberian Branch of Russian Academy of Sciences, Krasnoyarsk, Russian Federation.
Natalia O
NO
Zhila
Institute of Biophysics of Siberian Branch of Russian Academy of Sciences, Krasnoyarsk, Russian Federation.
Anatoly N
AN
Boyandin
Siberian Federal University, Krasnoyarsk, Russian Federation.
Daria A
DA
Syrvacheva
Siberian Federal University, Krasnoyarsk, Russian Federation.
Olga N
ON
Vinogradova
Institute of Biophysics of Siberian Branch of Russian Academy of Sciences, Krasnoyarsk, Russian Federation.
Galina S
GS
Kalacheva
L.V. Kirenckii Institute of Physics of Siberian Branch of Russian Academy of Sciences, Krasnoyarsk, Russian Federation.
Alexander D
AD
Vasiliev
Institute of Chemistry and Chemical Technology of Siberian Branch of Russian Academy of Sciences, Krasnoyarsk, Russian Federation.
Ivan V
IV
Peterson
D020155:3-Hydroxybutyric Acid / Q000737:chemistry; D001673:Biodegradation, Environmental; D019149:Bioreactors; D002244:Carbon / Q000737:chemistry; D002245:Carbon Dioxide / Q000737:chemistry*; D046970:Clostridium beijerinckii / Q000378:metabolism*; D003460:Crystallization; D005285:Fermentation; D005740:Gases; D006859:Hydrogen / Q000737:chemistry*; D010100:Oxygen / Q000737:chemistry; D054813:Polyhydroxyalkanoates / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry; D012037:Refuse Disposal / Q000379:methods; D013696:Temperature
D005740:Gases; D054813:Polyhydroxyalkanoates; D011108:Polymers; D002245:Carbon Dioxide; D002244:Carbon; D006859:Hydrogen; D010100:Oxygen; D020155:3-Hydroxybutyric Acid
Autotrophic synthesis; Cupriavidus eutrophus; Polyhydroxyalkanoates