Culturing and expansion of "clinical grade" precursors cells from the fetal human central nervous system.
NSCs have been demonstrated to be very useful in grafts into the mammalian central nervous system to investigate the exploitation of NSC for the therapy of neurodegenerative disorders in animal models of neurodegenerative diseases. To push cell therapy in CNS on stage of clinical application, it is necessary to establish a continuous and standardized, clinical grade (i.e., produced following the good manufacturing practice guidelines) human neural stem cell lines. In this chapter, we illustrate some of the protocols routinely used into our GMP cell bank for the production of "clinical grade" human neural stem cell lines.
Laboratorio Cellule Staminali, Cell Factory e Biobanca Azienda Ospedaliera Santa Maria di Terni, Terni, Italy.
Maurizio
M
Gelati
Daniela
D
Profico
Massimo
M
Projetti-Pensi
Gianmarco
G
Muzi
Giada
G
Sgaravizzi
Angelo Luigi
AL
Vescovi
D000818:Animals; D049109:Cell Proliferation; D002490:Central Nervous System / Q000166:cytology; D015925:Cryopreservation; D003470:Culture Media; D005333:Fetus / Q000166:cytology; D006801:Humans; D007621:Karyotyping; D051379:Mice; D008819:Mice, Nude; D058953:Neural Stem Cells / Q000502:physiology* / Q000637:transplantation; D061251:Primary Cell Culture / Q000592:standards*; D018874:Spheroids, Cellular / Q000502:physiology; D055153:Stem Cell Niche; D014015:Tissue Banks
D003470:Culture Media