Differential sensitivity of the species of Candida parapsilosis sensu lato complex against statins.
Candida parapsilosis sensu stricto, Candida orthopsilosis and Candida metapsilosis are human fungal pathogens with clinical importance. The recently reclassified three closely related species have significant variation in virulence, clinical prevalence and susceptibility characteristics to different antifungal compounds. The aim of this study was to investigate the in vitro activity of atorvastatin and fluvastatin against C. metapsilosis, C. orthopsilosis and C. parapsilosis. Susceptibility tests showed that C. parapsilosis was the most sensitive while C. orthopsilosis was the least susceptible species to both drugs. On the basis of the differential sensitivity, we developed a simple, reliable and highly cost-effective plate assay to distinguish these closely related species. Applying this method, 54 isolates belonging to the C. parapsilosis sensu lato complex deposited in Szeged Microbial Collection could be sorted into the three species with 100 % probability.
Department of Microbiology, Faculty of Science and Informatics, University of Szeged, Közép fasor 52, 6726, Szeged, Hungary.
Judit
J
Szenzenstein
Attila
A
Gácser
Zsuzsanna
Z
Grózer
Zoltán
Z
Farkas
Katalin
K
Nagy
Csaba
C
Vágvölgyi
János
J
Márki-Zay
Ilona
I
Pfeiffer
D000924:Anticholesteremic Agents / Q000494:pharmacology*; D000935:Antifungal Agents / Q000494:pharmacology*; D000069059:Atorvastatin; D002175:Candida / Q000145:classification / Q000187:drug effects* / Q000302:isolation & purification; D005229:Fatty Acids, Monounsaturated / Q000494:pharmacology*; D000077340:Fluvastatin; D006538:Heptanoic Acids / Q000494:pharmacology*; D006801:Humans; D007211:Indoles / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D008828:Microbiological Techniques / Q000379:methods; D011758:Pyrroles / Q000494:pharmacology*; D012680:Sensitivity and Specificity
D000924:Anticholesteremic Agents; D000935:Antifungal Agents; D005229:Fatty Acids, Monounsaturated; D006538:Heptanoic Acids; D007211:Indoles; D011758:Pyrroles; D000077340:Fluvastatin; D000069059:Atorvastatin