Silver nanoparticle-specific mitotoxicity in Daphnia magna.
Silver nanoparticles (Ag NPs) are gaining popularity as bactericidal agents in commercial products; however, the mechanisms of toxicity (MOT) of Ag NPs to other organisms are not fully understood. It is the goal of this research to determine differences in MOT induced by ionic Ag(+) and Ag NPs in Daphnia magna, by incorporating a battery of traditional and novel methods. Daphnia embryos were exposed to sublethal concentrations of AgNO3 and Ag NPs (130-650 ng/L), with uptake of the latter confirmed by confocal reflectance microscopy. Mitochondrial function was non-invasively monitored by measuring proton flux using self-referencing microsensors. Proton flux measurements revealed that while both forms of silver significantly affected proton efflux, the change induced by Ag NPs was greater than that of Ag(+). This could be correlated with the effects of Ag NPs on mitochondrial dysfunction, as determined by confocal fluorescence microscopy and JC-1, an indicator of mitochondrial permeability. However, Ag(+) was more efficient than Ag NPs at displacing Na(+) within embryonic Daphnia, based on inductively coupled plasma-mass spectroscopy (ICP-MS) analysis. The abnormalities in mitochondrial activity for Ag NP-exposed organisms suggest a nanoparticle-specific MOT, distinct from that induced by Ag ions. We propose that the MOT of each form of silver are complementary, and can act in synergy to produce a greater toxic response overall.
Department of Agriculture and Biological Engineering, Purdue University , West Lafayette, IN , USA.
Matthew C
MC
Stensberg
Rajtarun
R
Madangopal
Gowri
G
Yale
Qingshan
Q
Wei
Hugo
H
Ochoa-Acuña
Alexander
A
Wei
Eric S
ES
McLamore
Jenna
J
Rickus
D Marshall
DM
Porterfield
Maria S
MS
Sepúlveda
D000818:Animals; D003621:Daphnia / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism; D007928:Lethal Dose 50; D053768:Metal Nanoparticles / Q000737:chemistry / Q000633:toxicity*; D051336:Mitochondrial Membranes / Q000187:drug effects*; D010539:Permeability / Q000187:drug effects; D011522:Protons; D012834:Silver / Q000737:chemistry / Q000493:pharmacokinetics / Q000633:toxicity*; D012964:Sodium / Q000378:metabolism; D018675:Toxicity Tests
D011522:Protons; D012834:Silver; D012964:Sodium