Tips for the functionalization of nanoparticles with antibodies.
Multiple antibody immobilization methodologies have been developed for several applications including affinity chromatography, immunosensing, and drug delivery. Most of them have been carried out without considering the orientation of the antigen binding site of the antibody, or after the chemical modification of the antibody. An efficient immobilization to improve the biological activity of the antibody is one of the key fundamental issues to pursue. A simple and effective methodology for well-oriented covalently immobilization of antibodies on nanoparticles is reported in this chapter.
Nanotherapy and Nanodiagnostics Group (GN2), Instituto de Nanociencia de Aragón, Universidad de Zaragoza, Zaragoza, Spain.
Ester
E
Polo
Sara
S
Puertas
María
M
Moros
Pilar
P
Batalla
José M
JM
Guisán
Jesús M
JM
de la Fuente
Valeria
V
Grazú
D000327:Adsorption; D055910:Antibodies, Immobilized / Q000737:chemistry*; D003102:Colloids; D003432:Cross-Linking Reagents / Q000737:chemistry; D006735:Horseradish Peroxidase / Q000276:immunology; D006863:Hydrogen-Ion Concentration; D007526:Isoelectric Point; D053758:Nanoparticles / Q000737:chemistry*; D011485:Protein Binding; D055550:Protein Stability
D055910:Antibodies, Immobilized; D003102:Colloids; D003432:Cross-Linking Reagents; D006735:Horseradish Peroxidase