A simple method to extract DNA from hair shafts using enzymatic laundry powder.
A simple method to extract DNA from hair shafts was developed by using enzymatic laundry powder at the first step of the process. The whole extraction can be finished in less than 2 hours. The simple extraction reagent proposed here contains only two cheap components: ordinary enzymatic laundry powder and PCR buffer. After extraction, an ultra sensitive fluorescent nucleic acid stain, PicoGreen, was used for quantifying trace amount of double-stranded DNA in the solution extracted. For further validation of DNA extraction, four primers were employed to amplify DNA microsatellite loci. Both fluorescence spectroscopy and PCR results suggested that this method can extract DNA from hair shafts with good efficiency and repeatability. The study will greatly facilitate the use of hair shafts in future for DNA analyses on genome-wide scale.
Institute of Quality Standards and Testing Technology for Agro-products, Key Laboratory of Agro-product Quality and Safety, Chinese Academy of Agricultural Sciences, Beijing, China.
Zheng
Z
Guan
Yu
Y
Zhou
Jinchuan
J
Liu
Xiaoling
X
Jiang
Sicong
S
Li
Shuming
S
Yang
Ailiang
A
Chen
D000818:Animals; D002417:Cattle; D004247:DNA / Q000737:chemistry* / Q000302:isolation & purification; D003902:Detergents / Q000737:chemistry*; D006197:Hair / Q000737:chemistry*; D009930:Organic Chemicals / Q000737:chemistry; D016133:Polymerase Chain Reaction; D013050:Spectrometry, Fluorescence
D003902:Detergents; D009930:Organic Chemicals; C099476:PicoGreen; D004247:DNA