MiR-200a enhances the migrations of A549 and SK-MES-1 cells by regulating the expression of TSPAN1.
MicroRNA-200a (miR-200a) has been reported to regulate tumour progression in several tumours; however, little is known about its role in non-small cell lung cancer cells (NSCLCs). Here, we found that miR-200a was up-regulated in A549 and SK-MES-1 cells compared with normal lung cells HELF. By a series of gain-of-function and loss-offunction studies, over-expression of miR-200a was indicated to enhance cells migration, and its knock-down inhibited migration of cells in NSCLC cell lines. Furthermore, miR-200a was identified to induce TSPAN1 expression which was related to migration. TSPAN1 was proved to induce migration, and so up-regulation of TSPAN1 by miR-200a may explain why over-expressing miR-200a promotes NSCLC cells migration.
School of Life Science and Technology, China Pharmaceutical University, Nanjing, 210009, China.
Yaqing
Y
Chen
Wei
W
Peng
Yixiang
Y
Lu
Jianxin
J
Chen
York Yuanyuan
YY
Zhu
Tao
T
Xi
D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics* / Q000378:metabolism; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics*; D049109:Cell Proliferation; D015536:Down-Regulation; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D059470:Tetraspanins / Q000235:genetics* / Q000378:metabolism; D015854:Up-Regulation
C533896:MIRN200 microRNA, human; D035683:MicroRNAs; C508125:TSPAN1 protein, human; D059470:Tetraspanins