Specific biomolecule corona is associated with ring-shaped organization of silver nanoparticles in cells.
We correlate the localization of silver nanoparticles inside cells with respect to the cellular architecture with the molecular information in the vicinity of the particle surface by combining nanoscale 3D cryo-soft X-ray tomography (cryo-SXT) with surface-enhanced Raman scattering (SERS). The interaction of the silver nanoparticle surface with small molecules and biopolymers was monitored by SERS in vitro over time in living cells. The spectra indicate a stable, time-independent surface composition of silver nanoparticles, despite the changing environment in the endosomal structure. Cryo-SXT reveals a characteristic ring-shaped organization of the silver nanoparticles in endosomes of different cell types. The ring-like structures inside the endosomes suggest a strong association among silver particles and with membrane structures. The comparison of the data with those obtained with gold nanoparticles suggests that the interactions between the nanoparticles and with the endosomal component are influenced by the molecular composition of the corona.
Humboldt-Universität zu Berlin, Department of Chemistry, Brook-Taylor-Str. 2, 12489 Berlin, Germany. janina.kneipp@chemie.hu-berlin.de.
Daniela
D
Drescher
Peter
P
Guttmann
Tina
T
Büchner
Stephan
S
Werner
Gregor
G
Laube
Andrea
A
Hornemann
Basel
B
Tarek
Gerd
G
Schneider
Janina
J
Kneipp
D016475:3T3 Cells; D000818:Animals; D002460:Cell Line; D011992:Endosomes / Q000737:chemistry / Q000378:metabolism; D053768:Metal Nanoparticles / Q000737:chemistry*; D051379:Mice; D046529:Microscopy, Electron, Transmission; D012834:Silver / Q000737:chemistry*; D013059:Spectrum Analysis, Raman
D012834:Silver