[Attenuation of cardiac ischemia/reperfusion injury by transient low hydroperoxide pretreatment in rat].
OBJECTIVE
To investigate how transient low dose of hydroperoxide pretreatment prevents cardiac ischemia/reperfusion injury.


METHODS
SD rats were divided into 4 groups: sham operation (Sham), standard ischemia/reperfusion (I/R), ischemic preconditioning (IPC) and IR preceded by low H2O2 treatment. Cardiac function and injury parameter were compared among groups.


RESULTS
IPC protected reperfusion injury and improved cardiac function. Low H2O2 treatment played a role in cardioprotection similar to IPC. Low H2O2 was indeed generated in the early phase of simulated ischemia and attenuated cytochrome c release induced by high Ca2+ in isolated mitochondria.


CONCLUSIONS
Low H2O2 plays a critical role in cardioprotection probably by inhibiting mitochondrial permeability transition.
Tianjin Medical College, Tianjin 300222, China.
Run-He
RH
Feng
Jin-Cai
JC
Zhang
Xiao-Qian
XQ
Li
Hai-Li
HL
Sun
Bin
B
Liu
Yun-Zhen
YZ
Zhao
Xiang-Dong
XD
Tang
Jing
J
Li
D000818:Animals; D006861:Hydrogen Peroxide / Q000008:administration & dosage*; D019194:Ischemic Preconditioning / Q000379:methods*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000517:prevention & control*
D006861:Hydrogen Peroxide