Ketoprofen combined with artery graft entubulization improves functional recovery of transected peripheral nerves.
The objective was to assess the local effect of ketoprofen on sciatic nerve regeneration and functional recovery. Eighty healthy male white Wistar rats were randomized into four experimental groups of 20 animals each: In the transected group (TC), the left sciatic nerve was transected and nerve cut ends were fixed in the adjacent muscle. In the treatment group the defect was bridged using an artery graft (AG/Keto) filled with 10 microliter ketoprofen (0.1 mg/kg). In the artery graft group (AG), the graft was filled with phosphated-buffer saline alone. In the sham-operated group (SHAM), the sciatic nerve was exposed and manipulated. Each group was subdivided into four subgroups of five animals each and regenerated nerve fibres were studied at 4, 8, 12 and 16 weeks post operation. Behavioural testing, sciatic nerve functional study, gastrocnemius muscle mass and morphometric indices showed earlier regeneration of axons in AG/Keto than in AG group (p < 0.05). Immunohistochemical study clearly showed more positive location of reactions to S-100 in AG/Keto than in AG group. When loaded in an artery graft, ketoprofen improved functional recovery and morphometric indices of the sciatic nerve. Local usage of this easily accessible therapeutic medicine is cost saving and avoids the problems associated with systemic administration.
Department of Clinical Sciences, Faculty of Veterinary Medicine, Urmia University, Nazloo Road, Urmia 57153 1177, Iran; Department of Clinical Sciences, Faculty of Veterinary Medicine, Urmia Branch, Islamic Azad University, Urmia, Iran. Electronic address: r.mohammadi@urmia.ac.ir.
Rahim
R
Mohammadi
Department of Clinical Sciences, Faculty of Veterinary Medicine, Urmia University, Nazloo Road, Urmia 57153 1177, Iran.
Moein
M
Mehrtash
Department of Clinical Sciences, Faculty of Veterinary Medicine, Urmia Branch, Islamic Azad University, Urmia, Iran.
Nima
N
Nikonam
Department of Clinical Sciences, Faculty of Veterinary Medicine, Urmia Branch, Islamic Azad University, Urmia, Iran.
Moied
M
Mehrtash
Department of Veterinary Pathology, Western College of Veterinary Medicine, University of Saskatchewan, 52 Campus Drive, Saskatoon, Saskatchewan S7N 5B4, Canada.
Keyvan
K
Amini
D000287:Administration, Topical; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000008:administration & dosage / Q000627:therapeutic use*; D001012:Aorta, Abdominal / Q000637:transplantation*; D001369:Axons / Q000187:drug effects / Q000648:ultrastructure; D007660:Ketoprofen / Q000008:administration & dosage / Q000627:therapeutic use*; D008124:Locomotion / Q000502:physiology; D008297:Male; D018482:Muscle, Skeletal / Q000294:innervation / Q000473:pathology; D009412:Nerve Fibers / Q000187:drug effects / Q000648:ultrastructure; D009416:Nerve Regeneration / Q000187:drug effects*; D019635:Neurosurgical Procedures / Q000379:methods; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D020127:Recovery of Function / Q000187:drug effects / Q000502:physiology; D009418:S100 Proteins / Q000032:analysis / Q000187:drug effects; D012584:Sciatic Nerve / Q000187:drug effects / Q000601:surgery*; D013997:Time Factors; D054457:Tissue Scaffolds
D000894:Anti-Inflammatory Agents, Non-Steroidal; D009418:S100 Proteins; D007660:Ketoprofen
Ketoprofen; Nerve repair; Sciatic; Topical