Novel multifunctional biocompatible gelatin-oleic acid conjugate: self-assembled nanoparticles for drug delivery.
In this work, a novel, biocompatible conjugates of gelatin and oleic acid (GOC) were synthesized by a novel aqueous solvent-based method that overcame challenges of completely contrary solubility between gelatin and oleic acid (OA). The GO nanoparticles (GONs) and Paclitaxel encapsulated nanoparticles (PTX-GON) were prepared by self-assembly in water. These nanoparticles (NPs) were then conjugated with folic acid (FA) for targeting cervical cancer cells (Hela cells) and were characterized for their various physicochemical and pharmaceutical properties. Fourier transform infrared spectroscopy (FT-IR) and 1H NMR studies indicated the successful synthesis of GOC which showed low critical aggregation concentration in water (0.015 mg/ml). All NPs were stable in human blood serum and their mean diameters were below 300 nm suitable for passive targeting. Powder X-ray diffraction (PXRD) diffractograms showed the reduction in drug crystallinity and hence, leading to the solubility enhancement of PTX. The release of PTX from both PTX-GON and FA conjugated PTX-GON (PTX-FA-GON) was controlled for a long time. The cytotoxicity results demonstrated great advantages of PTX-FA-GON and PTX-GON over the conventional dosage form of pacliaxel (Taxol). These results, therefore, indicate that GOC is a promising material to prepare drug encapsulated NP as a controlled delivery system and PTX-FA-GON is a potential targeted delivery system for cancer therapy.
International University, Vietnam National University-Ho Chi Minh City 70000, Vietnam. thlphuong@hcmiu.edu.vn
Phuong Ha-Lien
PH
Tran
Thao Truong-Dinh
TT
Tran
Toi Van
TV
Vo
Chau Le-Ngoc
CL
Vo
Beom-Jin
BJ
Lee
D000970:Antineoplastic Agents / Q000008:administration & dosage* / Q000009:adverse effects / Q000493:pharmacokinetics; D001672:Biocompatible Materials / Q000737:chemistry*; D016503:Drug Delivery Systems; D004355:Drug Stability; D005780:Gelatin / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D008422:Materials Testing; D058726:Nanoconjugates / Q000737:chemistry*; D009369:Neoplasms / Q000188:drug therapy; D019301:Oleic Acid / Q000737:chemistry*
D000970:Antineoplastic Agents; D001672:Biocompatible Materials; D058726:Nanoconjugates; D019301:Oleic Acid; D005780:Gelatin