Proteomic analysis of kidneys from selenoprotein M transgenic rats in response to increased bioability of selenium.
BACKGROUND
To characterize changes in global protein expression in kidneys of transgenic rats overexpressing human selenoprotein M (SelM) in response to increased bioabivility of selenium (Sel), total proteins extracted from kidneys of 10-week-old CMV/hSelM Tg and wild-type rats were separated by 2-dimensional gel electrophoresis and measured for changes in expression.


RESULTS
Ten and three proteins showing high antioxidant enzymatic activity were up- and down-regulated, respectively, in SelM-overexpressing CMV/hSelM Tg rats compared to controls based on an arbitrary 2-fold difference. Up-regulated proteins included LAP3, BAIAP2L1, CRP2, CD73 antigen, PDGF D, KIAA143 homolog, PRPPS-AP2, ZFP313, HSP-60, and N-WASP, whereas down-regulated proteins included ALKDH3, rMCP-3, and STC-1. After Sel treatment, five of the up-regulated proteins were significantly increased in expression in wild-type rats, whereas there were no changes in CMV/hSelM Tg rats. Only two of the down-regulated proteins showed reduced expression in wild-type and Tg rats after Sel treatment.


CONCLUSIONS
These results show the primary novel biological evidences that new functional protein groups and individual proteins in kidneys of Tg rats relate to Sel biology including the response to Sel treatment and SelM expression.
Department of Biomaterials Science, College of Natural Resources & Life Science, Life and Industry Convergence Research Institute, Pusan National University, Miryang 627-706, South Korea.
Jun Seo
JS
Goo
Laboratory of Developmental Biology and Genomics, College of Veterinary Medicine, BK21 Program for Veterinary Science, Seoul National University, Seoul 151-742, South Korea.
Yo Na
YN
Kim
Laboratory of Developmental Biology and Genomics, College of Veterinary Medicine, BK21 Program for Veterinary Science, Seoul National University, Seoul 151-742, South Korea.
Kyung Mi
KM
Choi
Department of Biomaterials Science, College of Natural Resources & Life Science, Life and Industry Convergence Research Institute, Pusan National University, Miryang 627-706, South Korea.
In Sik
IS
Hwang
Department of Biomaterials Science, College of Natural Resources & Life Science, Life and Industry Convergence Research Institute, Pusan National University, Miryang 627-706, South Korea.
Ji Eun
JE
Kim
Department of Biomaterials Science, College of Natural Resources & Life Science, Life and Industry Convergence Research Institute, Pusan National University, Miryang 627-706, South Korea.
Young Ju
YJ
Lee
Department of Biomaterials Science, College of Natural Resources & Life Science, Life and Industry Convergence Research Institute, Pusan National University, Miryang 627-706, South Korea.
Moon Hwa
MH
Kwak
Department of Laboratory Animal Resources, National Institute of Food and Drug Safety, Korea FDA, Osong 363-700, Korea.
Sun Bo
SB
Shim
Department of Laboratory Animal Resources, National Institute of Food and Drug Safety, Korea FDA, Osong 363-700, Korea.
Seung Wan
SW
Jee
Department of Laboratory Animal Resources, National Institute of Food and Drug Safety, Korea FDA, Osong 363-700, Korea.
Chul Joo
CJ
Lim
Laboratory of Developmental Biology and Genomics, College of Veterinary Medicine, BK21 Program for Veterinary Science, Seoul National University, Seoul 151-742, South Korea.|Interdisciplinary Program for Bioinformatics, Program or Cancer Biology and BIO-MAX Institute, Seoul National University, Seoul 151-742, South Korea.
Je Kyung
JK
Seong
Department of Biomaterials Science, College of Natural Resources & Life Science, Life and Industry Convergence Research Institute, Pusan National University, Miryang 627-706, South Korea.
Dae Youn
DY
Hwang