Disintegration of aerobic granules: role of second messenger cyclic di-GMP.
Loss of structural stability of aerobic granular process is the challenge for its field applications to treat wastewaters. The second messenger, cyclic diguanylate (c-di-GMP), is widely used by bacteria to regulate the synthesis of exopolysaccharide. This study for the first time confirmed the correlation between concentration of intracellular c-di-GMP and the granular stability under sequencing batch reactor (MBR) mode. In the presence of manganese ions (Mn(2+)), the concentrations of intracellular c-di-GMP and of extracellular polysaccharides and proteins in granules were declined. Clone library study revealed that the polysaccharide producers. Acinetobacter sp., Thauera sp., Bdellovibrio sp. and Paracoccus sp. were lost after Mn(2+) addition. The findings reported herein confirmed that the c-di-GMP is a key chemical factor epistatic to quorum sensing to determine granular stability. Stimulation of synthesis of intracellular c-di-GMP presents a potential way to enhance long-term stability of aerobic granules.
Department of Environmental Science and Engineering, Fudan University, Shanghai 200433, China.
Chunli
C
Wan
Department of Environmental Science and Engineering, Fudan University, Shanghai 200433, China.
Peng
P
Zhang
Department of Environmental Science and Engineering, Fudan University, Shanghai 200433, China; Laboratory of Environmental Pollution and Bioremediation, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi, Xinjiang 830011, China; Department of Chemical Engineering, National Taiwan University, Taipei 106, Taiwan; Department of Chemical Engineering, National Taiwan University of Science and Technology, Taipei 106, Taiwan. Electronic address: djlee@ntu.edu.tw.
Duu-Jong
DJ
Lee
Laboratory of Environmental Pollution and Bioremediation, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi, Xinjiang 830011, China.
Xue
X
Yang
Department of Environmental Science and Engineering, Fudan University, Shanghai 200433, China.
Xiang
X
Liu
Department of Environmental Science and Engineering, Fudan University, Shanghai 200433, China.
Supu
S
Sun
Laboratory of Environmental Pollution and Bioremediation, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi, Xinjiang 830011, China.
Xiangliang
X
Pan
D000150:Acinetobacter / Q000378:metabolism; D000332:Aerobiosis; D001426:Bacterial Proteins / Q000737:chemistry; D001501:Bdellovibrio / Q000378:metabolism; D019149:Bioreactors; D006152:Cyclic GMP / Q000031:analogs & derivatives* / Q000737:chemistry; D004926:Escherichia coli / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D007220:Industrial Waste; D007477:Ions; D008345:Manganese / Q000737:chemistry; D010230:Paracoccus / Q000378:metabolism; D011134:Polysaccharides / Q000737:chemistry; D011506:Proteins / Q000737:chemistry; D053038:Quorum Sensing / Q000235:genetics / Q000502:physiology; D012336:RNA, Ribosomal, 16S / Q000378:metabolism; D015290:Second Messenger Systems / Q000235:genetics*; D012722:Sewage / Q000737:chemistry*; D020628:Thauera / Q000378:metabolism; D013997:Time Factors; D014865:Waste Disposal, Fluid; D062065:Wastewater
D001426:Bacterial Proteins; D006152:Cyclic GMP; D007220:Industrial Waste; D007477:Ions; D008345:Manganese; D011134:Polysaccharides; D011506:Proteins; D012336:RNA, Ribosomal, 16S; D012722:Sewage; D062065:Wastewater; C062025:bis(3',5')-cyclic diguanylic acid
Aerobic granule; Clone libraries; Cyclic di-GMP; Disintegration; Polysaccharide