Development of a peptide based latex agglutination assay for serotype identification of foot and mouth disease virus.
Out of 200 serum samples collected from cattle (142) and buffaloes (58) of various ages and sexand subjected to latex agglutination test (LAT) using serotype specific peptides (O, A, Asia 1) and also with peptide for non-structural protein 2B (NSP-2B), 114 (70%) samples were positive against FMDV type 'O', 102 (51%) against serotype 'A' and 104 (52%) against serotype 'Asia 1'. With NSP-2B peptide a total of 71 (35.5%) samples were positive. The results suggest that LAT could be used for the diagnosis of foot and mouth disease virus as it is easy, cheap and effective test.
Department of Veterinary Microbiology, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana 141004, India.
Dilpreet
D
Kaur
Gurpreet
G
Kaur
Mudit
M
Chandra
Hari M
HM
Saxena
Padam N
PN
Dwivedi
D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D005536:Foot-and-Mouth Disease / Q000276:immunology / Q000821:virology; D029544:Foot-and-Mouth Disease Virus / Q000145:classification*; D007841:Latex Fixation Tests / Q000379:methods*; D008863:Microspheres; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry / Q000276:immunology*; D012703:Serotyping; D014611:Vaccination; D017361:Viral Nonstructural Proteins / Q000276:immunology
D010455:Peptides; D017361:Viral Nonstructural Proteins