Identification of a novel chitin-binding spore wall protein (NbSWP12) with a BAR-2 domain from Nosema bombycis (microsporidia).
The spore wall of Nosema bombycis plays an important role in microsporidian pathogenesis. Protein fractions from germinated spore coats were analysed by two-dimensional polyacrylamide gel electrophoresis and MALDI-TOF/TOF mass spectrometry. Three protein spots were identified as the hypothetical spore wall protein NbHSWP12. A BAR-2 domain (e-value: 1.35e-03) was identified in the protein, and an N-terminal protein-heparin interaction motif, a potential N-glycosylation site, and 16 phosphorylation sites primarily activated by protein kinase C were also predicted. The sequence analysis suggested that Nbhswp12 and its homologous genes are widely distributed among microsporidia. Additionally, Nbhswp12 gene homologues share similar sequence features. An indirect immunofluorescence analysis showed that NbHSWP12 localized to the spore wall, and thus we renamed it spore wall protein 12 (NbSWP12). Moreover, NbSWP12 could adhere to deproteinized N. bombycis chitin coats that were obtained by hot alkaline treatment. This novel N. bombycis spore wall protein may function in a structural capacity to facilitate microsporidial spore maintenance.
State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, China.
Jie
J
Chen
Lina
L
Geng
Mengxian
M
Long
Tian
T
Li
Zhi
Z
Li
Donglin
D
Yang
Chao
C
Ma
Haijing
H
Wu
Zhengang
Z
Ma
Chunfeng
C
Li
Guoqing
G
Pan
Zeyang
Z
Zhou
D000595:Amino Acid Sequence; D002473:Cell Wall / Q000737:chemistry / Q000378:metabolism; D002686:Chitin / Q000378:metabolism*; D017124:Conserved Sequence; D015180:Electrophoresis, Gel, Two-Dimensional; D019084:Fluorescent Antibody Technique, Indirect; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D013058:Mass Spectrometry; D016818:Nosema / Q000737:chemistry / Q000166:cytology / Q000378:metabolism*; D010802:Phylogeny; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013172:Spores, Fungal; D014158:Transcription, Genetic
D005656:Fungal Proteins; D002686:Chitin