Nicotine induces alteration of H3K27 demethylase UTX in kidney cancer cell.
Cigarette smoking is one of the most important risk factors for kidney cancer, but the molecular mechanism is poorly understood. To examine the expression change of histone H3 on lysine 27 trimethylase (H3K27me3) demethylases ubiquitously transcribed TPR gene on the X chromosome (UTX) in kidney cancer cell line 786-O after nicotine treatment, quantitative real-time-polymerase chain reaction and western blotting analysis were carried out. These results showed that nicotine can increase UTX messenger RNA and protein levels and also decrease the content of H3K27me3. The decreased content of H3K27me3 may activate specific gene expression and lead to kidney cancer. Future investigation on nicotine induced UTX expression and its epigenetic effect would deepen our understanding on nicotine toxicity and carcinogenicity.
1Shenzhen Key Laboratory of Genitourinary Tumor, Shenzhen Second People's Hospital, Zhongshan School of Medicine, Sun Yat-sen University, First Affiliated Hospital of Shenzhen University, Shenzhen, Guangdong, People's Republic of China.
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Guo
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Li
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Wang
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Tian
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Duan
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Cai
D015153:Blotting, Western; D045744:Cell Line, Tumor; D015536:Down-Regulation / Q000187:drug effects; D000071221:Enhancer of Zeste Homolog 2 Protein; D056466:Histone Demethylases / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D056484:Jumonji Domain-Containing Histone Demethylases / Q000187:drug effects* / Q000378:metabolism*; D007680:Kidney Neoplasms / Q000473:pathology; D009538:Nicotine / Q000494:pharmacology*; D018722:Nicotinic Agonists / Q000494:pharmacology*; D009687:Nuclear Proteins / Q000187:drug effects / Q000378:metabolism*; D063151:Polycomb Repressive Complex 2 / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D015854:Up-Regulation / Q000187:drug effects
D018722:Nicotinic Agonists; D009687:Nuclear Proteins; D012333:RNA, Messenger; D009538:Nicotine; D056466:Histone Demethylases; D056484:Jumonji Domain-Containing Histone Demethylases; C523998:KDM6A protein, human; C524220:KDM6B protein, human; C498409:EZH2 protein, human; D000071221:Enhancer of Zeste Homolog 2 Protein; D063151:Polycomb Repressive Complex 2
Nicotine; UTX; histone demethylase; kidney cancer