Responses of macrophages against Salmonella infection compared with phagocytosis.
To explore the responses of host cell after infection with live Salmonella compared with phagocytosis to dead bacteria, the responses of mouse macrophage after infection with Salmonella enteritidis C50041 and the fixed C50041 (C50041-d) were analyzed. Results indicated that the cytotoxicity induced by C50041 was stronger than C50041-d. Similar changing trends of mitochondrial membrane potential, intracellular concentration of calcium ions, reactive oxygen species and nitric oxide were found between C50041 and C50041-d infection. But the cell responses against C50041 were earlier and stronger than C50041-d. LC3 expression of macrophage induced by C50041 was lower than C50041-d. C50041 significantly inhibited the production of tumor necrosis factor and interleukin (IL)-6. Whereas intracellular caspase-1 activation and IL-1β release induced by C50041 were stronger than C50041-d, caspase-1 activation and IL-1β release are the innate defense responses of macrophage. Therefore, it will be beneficial to explore the use of this pathway in the control of Salmonella infection.
Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, 225009, People's Republic of China.
Maozhi
M
Hu
Yun
Y
Yang
Chuang
C
Meng
Zhiming
Z
Pan
Xinan
X
Jiao
D000818:Animals; D002118:Calcium / Q000378:metabolism; D020170:Caspase 1 / Q000378:metabolism; D005260:Female; D005434:Flow Cytometry; D053583:Interleukin-1beta / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008164:Luminescent Proteins; D008264:Macrophages / Q000276:immunology*; D053078:Membrane Potential, Mitochondrial / Q000502:physiology; D051379:Mice; D008807:Mice, Inbred BALB C; D009569:Nitric Oxide / Q000378:metabolism; D010587:Phagocytosis / Q000276:immunology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D012481:Salmonella Infections, Animal / Q000276:immunology*; D012477:Salmonella enteritidis / Q000276:immunology*; D000097573:Red Fluorescent Protein
D002118:Calcium; D020170:Caspase 1; D053583:Interleukin-1beta; D007770:L-Lactate Dehydrogenase; D008164:Luminescent Proteins; D009569:Nitric Oxide; D017382:Reactive Oxygen Species; D000097573:Red Fluorescent Protein