Eclipsing thermal lens spectroscopy for fluorescence quantum yield measurement.
A modified spatial filtering method that improves the sensitivity of single-beam and mode-mismatched thermal lens spectroscopy (TLS) for fluorescence quantum yield measurement is presented. The method is based on the detection of the external part of a laser beam transmitted by the fluorescent sample (eclipsing detection mode). The experimental results show that the signal/noise (S/N) ratio of the absolute quantum yield of Rh6G can be enhanced up to ~1400% using the eclipsing detection mode on the TLS experimental setup. The method was evaluated by measuring the fluorescence quantum yield of varying concentration of ethanolic solutions of Rhodamine 6G.
Laboratory of Biomedical Optics and Imaging, Federal University of Pernambuco, Cidade Universitária, Recife-PE, 50740-530, Brazil.
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Estupiñán-López
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Tolentino Dominguez
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de Araujo
D017076:Computer-Aided Design; D004867:Equipment Design; D019544:Equipment Failure Analysis; D000431:Ethanol / Q000032:analysis*; D007909:Lenses; D008832:Microchemistry / Q000295:instrumentation*; D036103:Nanotechnology / Q000295:instrumentation*; D011789:Quantum Theory; D013050:Spectrometry, Fluorescence / Q000295:instrumentation*; D013696:Temperature
D000431:Ethanol