Bacterial cyclic AMP-phosphodiesterase activity coordinates biofilm formation.
Biofilm-related infections are a major contributor to human disease, and the capacity for surface attachment and biofilm formation are key attributes for the pathogenesis of microbes. Serratia marcescens type I fimbriae-dependent biofilms are coordinated by the adenylate cyclase, CyaA, and the cyclic 3',5'-adenosine monophosphate (cAMP)-cAMP receptor protein (CRP) complex. This study uses S. marcescens as a model system to test the role of cAMP-phosphodiesterase activity in controlling biofilm formation. Herein we describe the characterization of a putative S. marcescens cAMP-phosphodiesterase gene (SMA3506), designated as cpdS, and demonstrated to be a functional cAMP-phosphodiesterase both in vitro and in vivo. Deletion of cpdS resulted in defective biofilm formation and reduced type I fimbriae production, whereas multicopy expression of cpdS conferred a type I fimbriae-dependent hyper-biofilm. Together, these results support a model in which bacterial cAMP-phosphodiesterase activity modulates biofilm formation.
Charles T. Campbell Laboratory of Ophthalmic Microbiology, Department of Ophthalmology, University of Pittsburgh, Pittsburgh, Pennsylvania, Unites States of America.
Eric J
EJ
Kalivoda
Kimberly M
KM
Brothers
Nicholas A
NA
Stella
Matthew J
MJ
Schmitt
Robert M Q
RM
Shanks
D001419:Bacteria / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism*; D018441:Biofilms; D000242:Cyclic AMP / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism; D010861:Fimbriae, Bacterial / Q000378:metabolism / Q000648:ultrastructure; D015964:Gene Expression Regulation, Bacterial; D023061:Gene Order; D016680:Genome, Bacterial; D006868:Hydrolysis; D008969:Molecular Sequence Data; D009154:Mutation; D011953:Receptors, Cyclic AMP / Q000378:metabolism*; D012706:Serratia marcescens / Q000235:genetics / Q000254:growth & development / Q000378:metabolism
D029968:Escherichia coli Proteins; D011953:Receptors, Cyclic AMP; D000242:Cyclic AMP