Vinylsulfonamide and acrylamide modification of DNA for cross-linking with proteins.
Bioorthogonal covalent cross-linking of DNA-binding proteins (p53) to DNA was achieved through novel DNA probes bearing a reactive vinylsulfonamide (VS) group. The VS-modified dCTP served as building block for polymerase synthesis of modified DNA, which was readily conjugated with cysteine-containing peptides and proteins by Michael addition.
Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, Gilead Sciences & IOCB Research Center, Flemingovo nám. 2, 16610 Prague 6 (Czech Republic) http://www.uochb.cas.cz/hocekgroup.
Jitka
J
Dadová
Petr
P
Orság
Radek
R
Pohl
Marie
M
Brázdová
Miroslav
M
Fojta
Michal
M
Hocek
D020106:Acrylamide / Q000138:chemical synthesis / Q000737:chemistry*; D003432:Cross-Linking Reagents / Q000737:chemistry; D004247:DNA / Q000138:chemical synthesis / Q000737:chemistry*; D004259:DNA-Directed DNA Polymerase / Q000737:chemistry; D005030:Ethylenes / Q000737:chemistry; D008958:Models, Molecular; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism; D013449:Sulfonamides / Q000138:chemical synthesis / Q000737:chemistry*; D013451:Sulfonic Acids / Q000737:chemistry; D014753:Vinyl Compounds / Q000138:chemical synthesis / Q000737:chemistry*
D003432:Cross-Linking Reagents; D005030:Ethylenes; D011506:Proteins; D013449:Sulfonamides; D013451:Sulfonic Acids; D014753:Vinyl Compounds; D020106:Acrylamide; D004247:DNA; D004259:DNA-Directed DNA Polymerase; C085726:ethylenesulfonic acid
DNA; DNA polymerase; Michael additions; bioorthogonal chemistry; proteins