Immobilization of enzymes on monofunctional and heterofunctional epoxy-activated supports.
The immobilization of proteins on epoxy activated supports is discussed in this chapter. Immobilization on epoxy supports is carried out as a two-step mechanism: in the first step the adsorption of the protein is promoted and in the second step the intramolecular covalent linkage among epoxy groups and nucleophiles of the protein is produced. Based on this mechanism of the need of a first adsorption of the protein on the support, different epoxy supports are described. The different supports are able to immobilize proteins through different orientations being obtained catalysts with different properties of activity, stability, and selectivity.
Institute of Catalysis, CSIC, CAMPUS UAM-Cantoblanco, Madrid, Spain.
Cesar
C
Mateo
Valeria
V
Grazu
Jose M
JM
Guisan
D000327:Adsorption; D003432:Cross-Linking Reagents / Q000737:chemistry*; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry*; D004852:Epoxy Compounds / Q000737:chemistry*; D029968:Escherichia coli Proteins / Q000737:chemistry; D005656:Fungal Proteins / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007474:Ion Exchange; D010399:Penicillin Amidase / Q000737:chemistry; D011108:Polymers / Q000737:chemistry; D001616:beta-Galactosidase / Q000737:chemistry
D003432:Cross-Linking Reagents; D004800:Enzymes, Immobilized; D004852:Epoxy Compounds; D029968:Escherichia coli Proteins; D005656:Fungal Proteins; D011108:Polymers; C065843:Eupergit C; D001616:beta-Galactosidase; D010399:Penicillin Amidase