Preparation of C-terminally modified chemokines by expressed protein ligation.
In order to link structural features on a molecular level to the function of chemokines, site-specific modification strategies are strongly required. These can be used to incorporate fluorescent dyes and/or physical probes to allow investigations in a wide range of biological and physical techniques, e.g., nuclear magnetic resonance (NMR) spectroscopy, fluorescence microscopy, fluorescence resonance energy transfer (FRET), or fluorescence correlation spectroscopy (FCS). Only a limited number of functional groups within the 20 canonical amino acids allow ligation strategies that can be helpful to introduce novel functionalities, which in turn expand the scope of chemoselective and orthogonal reactivity of (semi)synthetic chemokines. In the present chapter we mainly focus on the fabulous history of native chemical ligation (NCL) and provide a general protocol for the preparation of C-terminally modified SDF-1α including tips and tricks for practical work. We believe that this protocol can be easily adapted to other chemokines and many proteins in general.
Institute of Biochemistry, Universität Leipzig, Leipzig, Germany.
Lars
L
Baumann
Max
M
Steinhagen
Annette G
AG
Beck-Sickinger
D018925:Chemokines / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D010446:Peptide Fragments / Q000138:chemical synthesis; D058849:Protein Refolding; D011506:Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D060327:Solid-Phase Synthesis Techniques / Q000379:methods
D018925:Chemokines; D010446:Peptide Fragments; D011506:Proteins