[Role of Rab23 in invasion and migration of human breast cancer Bcap-37 cells].
OBJECTIVE
To investigate the effects of Rab23 over-expression and Rab23 knock-out on the migration and invasion of breast cancer cell line Bcap-37.


METHODS
Western blotting was employed to detect the expression of Rab23 in breast cell line HBL-100 and cancer cell lines Bcap-37, MDA-MB-231, and MCF-7. Lentiviral vectors with Rab23 genes and with Rab23 RNAi were respectively transfected into breast cancer cell line Bcap-37, and the cells presenting the stable over-expression of Rab23 and the knock-out of Rab23 were respectively selected. Scratch healing assay and Transwell(R); invasion assay were used to examine the alterations of Bcap-37 cell migration and invasion potentials after Rab23 over-expression and Rab23 knock-out.


RESULTS
Western blotting indicated that Rab23 was expressed in all breast cancer cell lines and normal breast cell line. And Rab23 expression level was the highest in Bcap-37 cells, which was statistically different as compared with HBL-100 cells (P<0.01). Compared with the control group, Bcap-37 cell line with Rab23 over-expression had the dramatically enhanced migration and invasion potentials (P<0.01). On the other hand, Bcap-37 cell line with Rab23 knock-out showed the significantly attenuated migration and invasion potentials (P<0.01).


CONCLUSIONS
Rab23 plays a pivotal role in the migration and invasion of Bcap-37 cells.
Department of Physiology, Fourth Military Medical University, Xi'an, China.
Jie
J
Zhao
Yali
Y
Liu
Qiang
Q
Jian
Juan
J
Li
Sumin
S
Chi
D001943:Breast Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000502:physiology*; D005260:Female; D055786:Gene Knockout Techniques; D006801:Humans; D009361:Neoplasm Invasiveness; D020691:rab GTP-Binding Proteins / Q000096:biosynthesis / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*
C486614:RAB23 protein, human; D020691:rab GTP-Binding Proteins