[Choline promotes angiogenesis in chick embryo chorioallantoic membrane].
OBJECTIVE
To investigate choline promoting angiogenesis on chick embryo chorioallantoic membrane (CAM).


METHODS
CAM model was prepared, the choline chloride, human vascular endothelial growth factor (hVEGF) and normal saline were added respectively onto the carrier on the CAM, the state of angiogenesis was observed and the number of new blood vessels was counted.


RESULTS
Choline chloride was tested at the concentrations of 0.5 nmol/L - 1 mmol/L in this experiment, when its concentrations were increased to 0.01 micromol/L - 1 000 micromol/L, it could stimulate angiogenesis, the minimum effective concentration was tested as 0.01 micromol/L, and its effect for promoting the angiogenesis was equivalent to that of hVEGF, the potent stimulator for angiogenesis.


CONCLUSIONS
The result shows that choline can promote angiogenesis in the chick embryo chorioallantoic membrane.
The Affiliated Hospital, Academy of Military Medical Sciences, Beijing 100071, China.
Hui
H
Wang
Xiang-Zhi
XZ
Zeng
Wen-Yu
WY
Cui
Lian
L
Duan
D000818:Animals; D002642:Chick Embryo / Q000098:blood supply / Q000187:drug effects*; D002794:Choline / Q000494:pharmacology*; D049033:Chorioallantoic Membrane / Q000098:blood supply / Q000187:drug effects*; D018919:Neovascularization, Physiologic / Q000187:drug effects*
D002794:Choline