Simultaneous determination of six resorcylic acid lactones in feed using liquid chromatography-tandem mass spectrometry and multi-walled carbon nanotubes as a dispersive solid phase extraction sorbent.
A simple and cost-effective pre-treatment procedure was developed for six resorcylic acid lactones (RALs) in feed using dispersive solid phase extraction (dSPE) with multi-walled carbon nanotubes (MWCNTs). The sample was analysed after purification by ultra-high performance liquid chromatography-negative electrospray ionisation tandem mass spectrometry (UHPLC-ESI-MS/MS). After extraction with acetonitrile/water (80:20, v/v) and dilution with water, a dSPE procedure was carried out with MWCNTs. The pH value of the extract, the extraction time for MWCNTs, the type and amount of MWCNTs and the type of eluent were optimised to increase the sample throughput and the sensitivity. The samples were quantified using the internal standard zearalenone-D6. The absolute recoveries of the target compounds from feed samples were most efficient when using 100mg of MWCNTs with an outer diameter of less than 8nm and a length of 10-30μm, and ethyl acetate was shown to be the most suitable solvent for desorbing the target compounds from the MWCNTs. The mean recoveries from fortified swine mixed feed samples ranged from 95.3% to 107.2% and had relative standard deviations lower than 10%; the limits of detection and quantification for RALs were in the ranges of 0.20-0.29μg/kg and 0.54-0.78μg/kg, respectively.
Feed Science Institute, Zhejiang University, Hangzhou 310029, PR China.
Yong-Fei
YF
Ying
Yin-Liang
YL
Wu
Yi
Y
Wen
Ting
T
Yang
Xiu-Qin
XQ
Xu
Yi-Zhen
YZ
Wang
D000085:Acetates / Q000737:chemistry; D000097:Acetonitriles / Q000737:chemistry; D000821:Animal Feed / Q000032:analysis; D002853:Chromatography, Liquid / Q000379:methods*; D004968:Estrogens, Non-Steroidal / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D006863:Hydrogen-Ion Concentration; D057230:Limit of Detection; D016014:Linear Models; D037742:Nanotubes, Carbon / Q000737:chemistry; D015203:Reproducibility of Results; D052616:Solid Phase Extraction / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods*; D015025:Zearalenone / Q000031:analogs & derivatives* / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D015029:Zeranol / Q000031:analogs & derivatives* / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification
D000085:Acetates; D000097:Acetonitriles; D004968:Estrogens, Non-Steroidal; D037742:Nanotubes, Carbon; C029659:zearalenol; D015025:Zearalenone; C007650:ethyl acetate; D015029:Zeranol; C032159:acetonitrile
Feed; LC–MS/MS; MWCNTs; Resorcylic acid lactones; dSPE