[The expression of CARD18 in apoptin-transfected gastric cancer cells and gastric adenocarcinoma tissues].
OBJECTIVE
To study the expression of caspase recruitment domain family member 18 (CARD18) in apoptin-induced apoptosis of gastric cancer cells and its role in the development of gastric cancer.


METHODS
After gastric cancer cells were transfected with apoptin, differentially expressed proteins between the apoptin-expression SGC7901 group and the control group were seperated using two-dimensional gel electrophoresis and identified using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) and Mascot database (http: //www.matrixscience.com/). The expression level and location of CARD18 in 56 cases of gastric adenocarcinoma and adjacent cancer-free tissues were respectively detected by RT-PCR, Western blotting and immunohistochemistry to analyze the relationship between CARD18 and the clinical pathological characteristics.


RESULTS
RT-PCR and Western blotting showed that the level of CARD18 mRNA and protein was down-regulated the most significantly after apoptin treatment. The expression of CARD18 in gastric adenocarcinoma tissues was significantly higher than that in adjacent cancer-free tissues (P<0.05), and it was proved that the CARD18 expression was related to the gastric cancer lymph node metastasis and TNM stage (P<0.05). CONCLUSION CARD18 may be both a promising marker for prognosis and a target protein for treatment of gastric cancer.
Chongqing Medical University, Chongqing, China.
Cui
C
Tan
Shaoning
S
Liu
Zhanhua
Z
Xiang
D000230:Adenocarcinoma / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D017209:Apoptosis / Q000502:physiology; D051017:Apoptosis Regulatory Proteins / Q000096:biosynthesis* / Q000235:genetics; D045744:Cell Line, Tumor; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D013274:Stomach Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology
D051017:Apoptosis Regulatory Proteins