TDAG51 deficiency promotes oxidative stress-induced apoptosis through the generation of reactive oxygen species in mouse embryonic fibroblasts.
Apoptosis has an important role in maintaining tissue homeostasis in cellular stress responses such as inflammation, endoplasmic reticulum stress, and oxidative stress. T-cell death-associated gene 51 (TDAG51) is a member of the pleckstrin homology-like domain family and was first identified as a pro-apoptotic gene in T-cell receptor-mediated cell death. However, its pro-apoptotic function remains controversial. In this study, we investigated the role of TDAG51 in oxidative stress-induced apoptotic cell death in mouse embryonic fibroblasts (MEFs). TDAG51 expression was highly increased by oxidative stress responses. In response to oxidative stress, the production of intracellular reactive oxygen species was significantly enhanced in TDAG51-deficient MEFs, resulting in the activation of caspase-3. Thus, TDAG51 deficiency promotes apoptotic cell death in MEFs, and these results indicate that TDAG51 has a protective role in oxidative stress-induced cell death in MEFs.
Department of Microbiology and Molecular Biology, College of Bioscience and Biotechnology, Chungnam National University, Daejeon, Korea.
Eui-Soon
ES
Park
Juhyeok
J
Kim
Tae-uk
TU
Ha
Jong-Soon
JS
Choi
Kwan
K
Soo Hong
Jaerang
J
Rho
D000818:Animals; D017209:Apoptosis; D004622:Embryo, Mammalian / Q000166:cytology*; D005347:Fibroblasts / Q000201:enzymology / Q000378:metabolism* / Q000473:pathology; D005786:Gene Expression Regulation; D042541:Intracellular Space / Q000378:metabolism; D051379:Mice; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D016328:NF-kappa B / Q000378:metabolism; D018384:Oxidative Stress / Q000235:genetics; D017382:Reactive Oxygen Species / Q000378:metabolism*; D015398:Signal Transduction; D014157:Transcription Factors / Q000172:deficiency* / Q000235:genetics / Q000378:metabolism
D016328:NF-kappa B; C114072:Phlda1 protein, mouse; D017382:Reactive Oxygen Species; D014157:Transcription Factors; D020928:Mitogen-Activated Protein Kinases