Thylakoid membrane maturation and PSII activation are linked in greening Synechocystis sp. PCC 6803 cells.
Thylakoid membranes are typical and essential features of both chloroplasts and cyanobacteria. While they are crucial for phototrophic growth of cyanobacterial cells, biogenesis of thylakoid membranes is not well understood yet. Dark-grown Synechocystis sp. PCC 6803 cells contain only rudimentary thylakoid membranes but still a relatively high amount of phycobilisomes, inactive photosystem II and active photosystem I centers. After shifting dark-grown Synechocystis sp. PCC 6803 cells into the light, "greening" of Synechocystis sp. PCC 6803 cells, i.e. thylakoid membrane formation and recovery of photosynthetic electron transport reactions, was monitored. Complete restoration of a typical thylakoid membrane system was observed within 24 hours after an initial lag phase of 6 to 8 hours. Furthermore, activation of photosystem II complexes and restoration of a functional photosynthetic electron transport chain appears to be linked to the biogenesis of organized thylakoid membrane pairs.
Institut für Pharmazie und Biochemie, Johannes Gutenberg-Universität Mainz, 55128 Mainz, Germany.
Sandra
S
Barthel
Gábor
G
Bernát
Tobias
T
Seidel
Eva
E
Rupprecht
Uwe
U
Kahmann
Dirk
D
Schneider
D002734:Chlorophyll / Q000378:metabolism; D004579:Electron Transport / Q000528:radiation effects; D052836:Heterotrophic Processes / Q000528:radiation effects; D015151:Immunoblotting; D007700:Kinetics; D008027:Light; D010100:Oxygen / Q000378:metabolism; D010788:Photosynthesis / Q000528:radiation effects; D045331:Photosystem I Protein Complex / Q000378:metabolism; D045332:Photosystem II Protein Complex / Q000378:metabolism*; D052817:Phototrophic Processes / Q000528:radiation effects; D021122:Protein Subunits / Q000378:metabolism; D013050:Spectrometry, Fluorescence; D046939:Synechocystis / Q000166:cytology* / Q000378:metabolism* / Q000528:radiation effects / Q000648:ultrastructure; D020524:Thylakoids / Q000378:metabolism* / Q000528:radiation effects / Q000648:ultrastructure; D013997:Time Factors
D045331:Photosystem I Protein Complex; D045332:Photosystem II Protein Complex; D021122:Protein Subunits; D002734:Chlorophyll; D010100:Oxygen