Expression of multiple Transient Receptor Potential channel genes in murine 3T3-L1 cell lines and adipose tissue.
BACKGROUND
Calcium and its signaling have a role in adipogenesis. Transient Receptor Potential (TRP) channels are non-selective cation channels with a high permeability to calcium.


METHODS
In the present study the expression of multiple TRP channels on mouse 3T3-L1 preadipocyte and adipocyte cells, white (WAT) and brown (BAT) adipose tissues was investigated using real time PCR (RT-PCR).


RESULTS
TRPV1, TRPV3, TRPM8, TRPC4, TRPC6 were differentially expressed in preadipocytes and adipocytes suggesting their significance in adipogenesis. Genes for multiple TRP channels were also expressed in murine WAT and BAT, out of which TRPV4, TRPV6 and TRPC6 showed differential expression.


CONCLUSIONS
Present study demonstrates the expression of TRP channels in mouse cell lines and adipose tissues.
National Agri-Food Biotechnology Institute (NABI), SAS Nagar (Mohali)-160071, Punjab, India. mbishnoi@gmail.com
Mahendra
M
Bishnoi
Kanthi Kiran
KK
Kondepudi
Aakriti
A
Gupta
Aniket
A
Karmase
Ravneet K
RK
Boparai
D041721:3T3-L1 Cells; D017667:Adipocytes / Q000378:metabolism*; D050156:Adipogenesis / Q000235:genetics*; D000273:Adipose Tissue / Q000378:metabolism*; D000818:Animals; D002460:Cell Line; D051379:Mice; D050051:Transient Receptor Potential Channels / Q000235:genetics* / Q000378:metabolism*
D050051:Transient Receptor Potential Channels