In vitro evaluation of plant-derived agents to preserve dentin collagen.
OBJECTIVE
Biomodification of dentin by a natural crosslinker has been recommended to improve a mechanical property of demineralized dentin. This study investigated the effect of various plant-derived agents (hesperidin, proanthocyanidin, epigallocatechin gallate and genipin) on the stability of dentin collagen matrix to resist collagenase degradation.


METHODS
The dentin specimens were treated with glutaraldehyde (0.5% and 5.0%) and each plant-derived test solution (0.5%). They were subjected to ultimate tensile strength (UTS) and swelling ratio measurements. Demineralized human dentin powder was incubated with 0.02%, 0.1% and 0.5% of each test agent and followed by bacterial collagenase digestion. The extent of collagen degradation was investigated using hydroxyproline assay.


RESULTS
The UTS and swelling ratio measurements revealed that the mechanical property of dentin was improved by the use of these natural agents. The greatest reduction in collagen degradation was shown following the use of hesperidin, proanthocyanidin, and epigallocatechin gallate at 0.5%.


CONCLUSIONS
The use of hesperidin, proanthocyanidin, and epigallocatechin gallate could improve the mechanical properties of collagen and resist enzymatic degradation, leading to functional repair of pathological dentin lesion.
Cariology and Operative Dentistry, Department of Oral Health Sciences, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Tokyo, Japan. Electronic address: hiraope@tmd.ac.jp.
Noriko
N
Hiraishi
Ryohei
R
Sono
Islam
I
Sofiqul
Cynthia
C
Yiu
Hiroyuki
H
Nakamura
Masayuki
M
Otsuki
Tsutomu
T
Takatsuka
Junji
J
Tagami
D003094:Collagen / Q000737:chemistry*; D003804:Dentin / Q000737:chemistry*; D006801:Humans; D066298:In Vitro Techniques; D010944:Plants / Q000737:chemistry*; D013718:Tensile Strength
D003094:Collagen
Dentin collagen; Epigallocatechin gallate; Genipin; Glutaraldehyde; Hesperidin; Proanthocyanidin