Grafting of poly-L-lysine dendrigrafts onto polypropylene surface using plasma activation for ATP immobilization - Nanomaterial for potential applications in biotechnology.
The present work describes a new environmental friendly strategy for the development of surfaces with high amine density via the grafting of native or modified poly-L-lysine dendrigraft (DGL G3) onto plasma activated polypropylene (PP), polystyrene (PS), polyimide, and polytetrafluoroethylene (PTFE) surface. Modified DGL G3 was prepared by replacement of few peripheral amines by various functionalities. Grafting efficiency was determined by wettability measurements, IRTF, XPS, AFM, and by colorimetry using optimized Coomassie Brilliant Blue method tailored for surface analysis. It was shown that a 4-7nm DGL G3 monolayer with 4×10(14)aminecm(-)(2) was covalently grafted onto various surfaces. Immobilization of adenosine triphosphate on the DGL-g-PP material from dilute solution was studied by bioluminescence and proved the ability of the material to interact with polyanionic biological compounds: 1 ATP complex with 5 amine groups. So, this material has a potential use in diagnostic and more widely for biotechnology due to its high capacity for biomolecule immobilization.
Institut Charles Gerhardt, Montpellier, UMR 5253 CNRS-UM2-ENSCM-UM1, Equipe Ingénierie et Architectures Macromoléculaires, Université Montpellier 2, cc1702, Place Eugène Bataillon, 34095 Montpellier cedex 5, France.
Benoit
B
Couturaud
Andrea
A
Molero Bondia
Clément
C
Faye
Laurent
L
Garrelly
André
A
Mas
Jean Jacques
JJ
Robin
D000255:Adenosine Triphosphate / Q000737:chemistry*; D001709:Biotechnology; D009682:Magnetic Resonance Spectroscopy; D018625:Microscopy, Atomic Force; D049329:Nanostructures; D011107:Polylysine / Q000737:chemistry*; D011126:Polypropylenes / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D013499:Surface Properties; D017155:Wettability
D011126:Polypropylenes; D011107:Polylysine; D000255:Adenosine Triphosphate
Biological applications of polymers; Biomaterials; Dendrimers; Monolayer; Surfaces