Molecular cloning and functional characterization of the dual oxidase (BmDuox) gene from the silkworm Bombyx mori.
Reactive oxygen species (ROS) from nicotinamide adenine dinucleotide phosphate (NADPH) oxidases and their related dual oxidases are known to have significant roles in innate immunity and cell proliferation. In this study, the 5,545 bp cDNA of the silkworm Bombyx mori dual oxidase (BmDuox) gene containing a full-length open reading frame was cloned. It was shown to include an N-terminal signal peptide consisting of 28 amino acid residues, a 240 bp 5'-terminal untranslated region (5'-UTR), an 802 bp 3'-terminal region (3'-UTR), which contains nine ATTTA motifs, and a 4,503 bp open reading frame encoding a polypeptide of 1,500 amino acid residues. Structural analysis indicated that BmDuox contains a typical peroxidase domain at the N-terminus followed by a calcium-binding domain, a ferric-reducing domain, six transmembrane regions and binding domains for flavin adenine dinucleotide (FAD) and nicotinamide adenine dinucleotide (NAD). Transcriptional analysis revealed that BmDuox mRNA was expressed more highly in the head, testis and trachea compared to the midgut, hemocyte, Malpighian tube, ovary, fat bodies and silk glands. BmDuox mRNA was expressed during all the developmental stages of the silkworm. Subcellular localization revealed that BmDoux was present mainly in the periphery of the cells. Some cytoplasmic staining was detected, with rare signals in the nucleus. Expression of BmDuox was induced significantly in the larval midgut upon challenge by Escherichia coli and Bombyx mori nucleopolyhedrovirus (BmNPV). BmDuox-deleted larvae showed a marked increase in microbial proliferation in the midgut after ingestion of fluorescence-labeled bacteria compared to the control. We conclude that reducing BmDuox expression greatly increased the bacterial load, suggesting BmDuox has an important role in inhibiting microbial proliferation and the maintenance of homeostasis in the silkworm midgut.
College of Animal Sciences, Zhejiang University, Hangzhou, China.
Xiaolong
X
Hu
Rui
R
Yang
Xing
X
Zhang
Lin
L
Chen
Xingwei
X
Xiang
Chengliang
C
Gong
Xiaofeng
X
Wu
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015153:Blotting, Western; D012831:Bombyx / Q000201:enzymology* / Q000235:genetics / Q000382:microbiology; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D004926:Escherichia coli / Q000472:pathogenicity; D004927:Escherichia coli Infections / Q000201:enzymology / Q000235:genetics / Q000382:microbiology*; D005182:Flavin-Adenine Dinucleotide / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D018507:Gene Expression Regulation, Developmental; D019476:Insect Proteins / Q000235:genetics / Q000378:metabolism*; D007814:Larva / Q000254:growth & development / Q000378:metabolism* / Q000382:microbiology; D008969:Molecular Sequence Data; D031625:Morus / Q000737:chemistry; D009243:NAD / Q000378:metabolism; D019255:NADPH Oxidases / Q000235:genetics / Q000378:metabolism*; D010802:Phylogeny; D018515:Plant Leaves / Q000201:enzymology / Q000382:microbiology; D012333:RNA, Messenger / Q000235:genetics; D017382:Reactive Oxygen Species / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013347:Subcellular Fractions
D018076:DNA, Complementary; D019476:Insect Proteins; D012333:RNA, Messenger; D017382:Reactive Oxygen Species; D009243:NAD; D005182:Flavin-Adenine Dinucleotide; D019255:NADPH Oxidases