Analysis of herpes simplex virion tegument ICP4 derived from infected cells and ICP4-expressing cells.
ICP4 is the major transcriptional regulatory protein of herpes simplex virus (HSV). It is expressed in infected cells with immediate early kinetics and is essential for viral growth. ICP4 is also a structural component of the virion tegument layer. Herpesviral tegument proteins exert regulatory functions important for takeover of the host cell. Tegument ICP4 has not been well characterized. We examined the ICP4 present in HSV-1 virions that were either derived from wild type infected cells or from ICP4-expressing (E5) cells infected with ICP4 deletion virus d120. Limited proteolysis demonstrated that virion-associated ICP4 from particles derived from E5 cells was indeed an internal component of the virion. A similar subset of virion structural proteins was detected in viral particles regardless of the cellular origin of ICP4. Genotypically ICP4-negative virions complemented with tegument ICP4 entered cells via a proteasome-dependent, pH-dependent pathway similar to wild type virions. In infected cells, ICP4 was distributed predominantly in intranuclear replication compartments regardless of whether it was expressed from a transgene or from the HSV genome.
Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington, United States of America.
Suzanne M
SM
Pritchard
Cristina W
CW
Cunha
Anthony V
AV
Nicola
D000818:Animals; D016466:CHO Cells; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D006224:Cricetinae; D003412:Cricetulus; D006561:Herpes Simplex / Q000235:genetics / Q000473:pathology* / Q000821:virology; D006801:Humans; D017874:Immediate-Early Proteins / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D018139:Simplexvirus / Q000235:genetics* / Q000378:metabolism; D014018:Tissue Distribution; D019076:Transgenes; D014709:Vero Cells; D014771:Virion / Q000378:metabolism
D017874:Immediate-Early Proteins; C048925:herpes simplex virus, type 1 protein ICP4