¹H, ¹⁵N, and ¹³C chemical shift assignments for the winged helix domains of two archeal MCM C-termini.
High-fidelity replication guarantees the stable inheritance of genetic information stored in the DNA of living organisms. The minichromosome maintenance (MCM) complex functions as replicative DNA-unwinding helicase and has been identified as one key player in the replication process of archea and eukarya. Despite the availability of considerable structural information on archeal MCMs, such information was missing for their C-terminal domain. In order to obtain more detailed structural information, we assigned the NMR chemical shifts for backbone and side chain nuclei for the MCM C-terminal winged helix domains of the archeal species Methanothermobacter thermautrophicus and Sulfolobus solfataricus.
Biomolecular NMR Spectroscopy, Leibniz Institute for Age Research-Fritz Lipman Institute, Beutenbergstr. 11, 07745, Jena, Germany, cwiede@fli-leibniz.de.
Christoph
C
Wiedemann
Oliver
O
Ohlenschläger
Barbara
B
Medagli
Silvia
S
Onesti
Matthias
M
Görlach
D000595:Amino Acid Sequence; D017013:Methanobacteriaceae / Q000201:enzymology*; D064110:Minichromosome Maintenance Proteins / Q000737:chemistry*; D008969:Molecular Sequence Data; D019906:Nuclear Magnetic Resonance, Biomolecular; D017434:Protein Structure, Tertiary; D048229:Sulfolobus solfataricus / Q000201:enzymology*
D064110:Minichromosome Maintenance Proteins