[The expressions of NLRP3 inflammasome and its downstream molecules in the mouse model of Helicobacter pylori infection].
OBJECTIVE
To study the expressions of NLRP3 inflammasome and its downstream molecules in the gastric tissues and sera of Helicobacter pylori (H.pylori)-infected C57BL/6 mice, and to preliminary explore the role of NLRP3 inflammasome signalling pathway in the pathogenesis of H.pylori infection.


METHODS
C57BL/6 mice were randomly divided into H.pylori infection group and PBS control group, and mice were sacrificed at different time points after H.pylori infection. The mRNA expressions of NLRP3 inflammasome and downstream signalling pathway related molecules in mouse gastric tissues were detected by RT-PCR, and the protein level of caspase-1 was analyzed by Western blotting. The contents of IL-1β, IL-18 and IL-33 in the sera were measured with ELISA.


RESULTS
Chronic inflammatory response was observed in the gastric mucosal tissues of H.pylori-infected mice and it gradually aggravated. Compared with the control mice, the mRNA expressions of NLRP3 inflammasome signalling pathway related molecules and the protein level of caspase-1 increased markedly in the gastric tissues of H.pylori-infected mice. Moreover, the contents of IL-1β, IL-18 and IL-33 in the sera of H.pylori-infected mice were also significantly elevated.


CONCLUSIONS
NLRP3 inflammasome signalling pathway could be activated by H.pylori infection.
Institute of Pathobiology, University of South China, Hengyang, China. jjyhebei@126.com
Jianye
J
Jiang
Sheng
S
Liu
Jingjing
J
Luo
Xiang
X
Li
Shuangyang
S
Tang
Minjun
M
Yu
Hengling
H
Cai
Tian
T
Tian
Yan
Y
Zhang
D000818:Animals; D002352:Carrier Proteins / Q000096:biosynthesis* / Q000235:genetics* / Q000276:immunology; D016481:Helicobacter Infections / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D016480:Helicobacter pylori / Q000276:immunology*; D058847:Inflammasomes / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D007378:Interleukins / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D000071199:NLR Family, Pyrin Domain-Containing 3 Protein; D015398:Signal Transduction
D002352:Carrier Proteins; D058847:Inflammasomes; D007378:Interleukins; D000071199:NLR Family, Pyrin Domain-Containing 3 Protein; C492716:Nlrp3 protein, mouse