Metabolomic assays of amoxicillin, cephapirin and ceftiofur in chicken muscle: application to treated chicken samples by liquid chromatography quadrupole time-of-flight mass spectrometry.
The aim of this study was to identity metabolites and transformation products (TPs) in chicken muscle from amoxicillin (AMX), cephapirin (PIR) and ceftiofur (TIO), which are antibiotics of the β-lactam family. Liquid chromatography coupled to quadrupole time-of-flight (QqTOF) mass spectrometry was utilized due to its high resolution, high mass accuracy and MS/MS capacity for elemental composition determination and structural elucidation. Amoxicilloic acid (AMA) and amoxicillin diketopiperazine (DKP) were found as transformation products from AMX. Desacetylcephapirin (DAC) was detected as a metabolite of PIR. Desfuroylceftiofur (DFC) and its conjugated compound with cysteine (DFC-S-Cys) were detected as a result of TIO in contact with chicken muscle tissue. The metabolites and transformation products were also monitored during the in vivo AMX treatment and slaughtering period. It was found that two days were enough to eliminate AMX and associated metabolites/transformation products after the end of administration.
Department de Química Analitica, Campus de l'Alimentació de Torribera, University of Barcelona, Avda. Prat de la Riba, 171, Sta. Coloma de Gramenet, E-08921 Barcelona, Spain.
M P
MP
Hermo
P
P
Gómez-Rodríguez
J
J
Barbosa
D
D
Barrón
D000658:Amoxicillin / Q000378:metabolism*; D000818:Animals; D001711:Biotransformation; D002511:Cephalosporins / Q000378:metabolism*; D002514:Cephapirin / Q000378:metabolism*; D002645:Chickens / Q000378:metabolism*; D002853:Chromatography, Liquid / Q000379:methods*; D004355:Drug Stability; D006863:Hydrogen-Ion Concentration; D055432:Metabolomics / Q000379:methods*; D009132:Muscles / Q000378:metabolism*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D013696:Temperature
D002511:Cephalosporins; D000658:Amoxicillin; C053503:ceftiofur; D002514:Cephapirin
Chicken muscle; Metabolites; Quadrupole time-of-flight mass spectrometry; Transformation products; β-Lactams