Synthesis of O-glycopeptides and construction of glycopeptide microarrays.
O-glycosylation of proteins is an important modification which affects biological function and immunity. In this chapter, we provide protocols for efficient solid-phase O-glycopeptide synthesis (SPGPS) and protocols for the construction of glycopeptide microarray chips for screening applications. This will be exemplified for mucin-type glycopeptides and the construction of glycopeptide microarrays. To this end, the protocols provided are particularly suited for small-scale robotic parallel synthesis. N-Terminal amine capping of deletion peptides during synthesis stands out as vital to this strategy. It allows for direct on-slide enrichment of the full-length target product and thereby bypasses tedious isolation and purification procedures.
Department of Chemistry, Faculty of Sciences, University of Copenhagen, Copenhagen, Denmark.
Ola
O
Blixt
Emiliano
E
Cló
D000596:Amino Acids / Q000737:chemistry; D005449:Fluorenes / Q000737:chemistry; D006020:Glycopeptides / Q000138:chemical synthesis* / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry; D040081:Protein Array Analysis / Q000379:methods*; D060327:Solid-Phase Synthesis Techniques / Q000379:methods*
D000596:Amino Acids; D005449:Fluorenes; D006020:Glycopeptides; D011134:Polysaccharides