Poly(N-isopropylacrylamide-co-acrylic acid) nanogels for tracing and delivering genes to human mesenchymal stem cells.
Drugs, proteins, and cells can be macro- and micro-encapsulated by unique materials that respond to specific stimuli. The phases and hydrophobic interactions of these materials are reversibly altered by environmental stimuli such as pH and temperature. These changes can lead to self-assembly of the materials, which enables controlled drug release and safe gene delivery into cells and tissues. The fate of stem cells delivered by such methods is of great interest. The formation of transgenic tissues requires genes to be delivered safely into stem cells. A cell tracing vehicle and a gene delivery carrier were simultaneously introduced into human mesenchymal stem cells (hMSCs). A thermo-sensitive hydrogel, poly(N-isopropylacrylamide-co-acrylic acid) (p(NiPAAm-co-AAc)), was created to generate self-assembled nanoparticles with nanogel characteristics. Hydrophobic interactions mediated the binding of the carboxyl group on the outside of p(NiPAAm-co-AAc) with the amine group of iron oxide. Nanogels carrying iron oxide and a fluorescent dye were complexed with specific genes. These nanogels could be internalized by hMSCs, and the transplantation of these cells into mice was monitored by in vivo imaging. Self-assembled p(NiPAAm-co-dAAc) nanogels complexed with green fluorescent protein were highly expressed in hMSCs and are a potential material for gene delivery.
Department of Biomedical Science, College of Life Science, CHA University, 3F, Yatap Acecore, 502 Yatap-dong Bundang-gu, Seongnam-si, Republic of Korea.
Ji Sun
JS
Park
Han Na
HN
Yang
Dae Gyun
DG
Woo
Su Yeon
SY
Jeon
Keun-Hong
KH
Park
D000178:Acrylamides / Q000737:chemistry*; D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D005260:Female; D005269:Femur / Q000166:cytology / Q000378:metabolism; D005290:Ferric Compounds / Q000378:metabolism; D018014:Gene Transfer Techniques; D057809:HEK293 Cells; D006367:HeLa Cells; D006801:Humans; D020100:Hydrogels / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008855:Microscopy, Electron, Scanning; D000080385:Nanogels; D010316:Particle Size; D011092:Polyethylene Glycols / Q000737:chemistry*; D011094:Polyethyleneimine / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D013696:Temperature; D014162:Transfection / Q000379:methods; D055815:Young Adult
D000178:Acrylamides; D005290:Ferric Compounds; D020100:Hydrogels; C090629:N-isopropylacrylamide-acrylic acid copolymer; D000080385:Nanogels; D011108:Polymers; C413692:polyethylene glycol polyethyleneimine nanogel; C000499:ferric oxide; D011092:Polyethylene Glycols; D011094:Polyethyleneimine
Gene; In vivo imaging; Stimuli-responsive; hMSC; p(NiPAAm-co-AAc)