Carprofen-imprinted monolith prepared by reversible addition-fragmentation chain transfer polymerization in room temperature ionic liquids.
To obtain fast separation, ionic liquids were used as porogens first in combination with reversible addition-fragmentation chain transfer (RAFT) polymerization to prepare a new type of molecularly imprinted polymer (MIP) monolith. The imprinted monolithic column was synthesized using a mixture of carprofen (template), 4-vinylpyridine, ethylene glycol dimethacrylate, [BMIM]BF4, and chain transfer agent (CTA). Some polymerization factors, such as template-monomer molar ratio, the degree of crosslinking, the composition of the porogen, and the content of CTA, on the column efficiency and imprinting effect of the resulting MIP monolith were systematically investigated. Affinity screening of structurally similar compounds with the template can be achieved in 200 s on the MIP monolith due to high column efficiency (up to 12,070 plates/m) and good column permeability. Recognition mechanism of the imprinted monolith was also investigated.
Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnostics (Theranostics), School of Pharmacy, Tianjin Medical University, Tianjin, 300070, China.
Lu
L
Ban
Xu
X
Han
Xian-Hua
XH
Wang
Yan-Ping
YP
Huang
Zhao-Sheng
ZS
Liu
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000737:chemistry / Q000302:isolation & purification*; D002227:Carbazoles / Q000737:chemistry / Q000302:isolation & purification*; D002846:Chromatography, Affinity / Q000379:methods*; D005504:Food Analysis / Q000379:methods; D052578:Ionic Liquids / Q000737:chemistry*; D008689:Methacrylates / Q000737:chemistry; D008892:Milk / Q000737:chemistry; D054802:Molecular Imprinting / Q000379:methods*; D058105:Polymerization; D011725:Pyridines / Q000737:chemistry; D013696:Temperature
D000894:Anti-Inflammatory Agents, Non-Steroidal; D002227:Carbazoles; D052578:Ionic Liquids; D008689:Methacrylates; D011725:Pyridines; C004919:ethylene dimethacrylate; C007005:carprofen; C029351:4-vinylpyridine