The little elongation complex functions at initiation and elongation phases of snRNA gene transcription.
The small nuclear RNA (snRNA) genes have been widely used as a model system for understanding transcriptional regulation due to the unique aspects of their promoter structure, selectivity for either RNA polymerase (Pol) II or III, and because of their unique mechanism of termination that is tightly linked with the promoter. Recently, we identified the little elongation complex (LEC) in Drosophila that is required for the expression of Pol II-transcribed snRNA genes. Here, using Drosophila and mammalian systems, we provide genetic and molecular evidence that LEC functions in at least two phases of snRNA transcription: an initiation step requiring the ICE1 subunit, and an elongation step requiring ELL.
Stowers Institute for Medical Research, 1000 East 50th Street, Kansas City, MO 64110, USA.
Deqing
D
Hu
Edwin R
ER
Smith
Alexander S
AS
Garruss
Nima
N
Mohaghegh
Joseph M
JM
Varberg
Chengqi
C
Lin
Jessica
J
Jackson
Xin
X
Gao
Anita
A
Saraf
Laurence
L
Florens
Michael P
MP
Washburn
Joel C
JC
Eissenberg
Ali
A
Shilatifard
D000818:Animals; D047369:Chromatin Immunoprecipitation; D004330:Drosophila / Q000235:genetics* / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D005786:Gene Expression Regulation; D045325:HCT116 Cells; D006801:Humans; D047468:Immunoprecipitation; D011401:Promoter Regions, Genetic; D012319:RNA Polymerase II / Q000235:genetics / Q000378:metabolism; D012320:RNA Polymerase III / Q000235:genetics / Q000378:metabolism; D012342:RNA, Small Nuclear / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction; D012045:Regulatory Sequences, Nucleic Acid; D012469:Salivary Glands / Q000166:cytology / Q000378:metabolism; D061805:Transcription Elongation, Genetic; D061785:Transcription Initiation, Genetic
D012342:RNA, Small Nuclear; D012319:RNA Polymerase II; D012320:RNA Polymerase III