Generation of a chemical gradient across an array of 256 cell cultures in a single chip.
A microfluidic diffusion diluter to create stable chemical gradients across an array of cell cultures was demonstrated. The device enabled concentration based studies to be conducted at 256 different concentrations across individual, low shear cell cultures. A gradient of staurosporine on cells stained with Mitotracker Deep Red (MTDR) showed a concentration-based effect on cell apoptosis across the cell culture array.
Department of Chemistry and Biochemistry, Texas Tech University, Lubbock, TX 79409, USA. d.pappas@ttu.edu.
Himali
H
Somaweera
Akif
A
Ibragimov
Dimitri
D
Pappas
D018929:Cell Culture Techniques / Q000295:instrumentation / Q000379:methods*; D002470:Cell Survival / Q000502:physiology; D006801:Humans; D046228:Microarray Analysis / Q000295:instrumentation / Q000379:methods*; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods*