Reproducibility of positive results for the detection of serum galactomannan by Platelia™ aspergillus EIA.
Galactomannan (GM) was recently included in consensus guidelines as an indirect mycological criterion for the diagnosis of invasive aspergillosis. Currently, there is an enzyme immunoassay available to detect GM in biological samples, the Platelia™ Aspergillus EIA. In this study, the reproducibility of positive results obtained using this assay was evaluated using serum samples from neutropenic patients. A trend toward lower values was observed, and 55 %(27/49) of positive results were negative after retesting. A low reproducibility of positive results for the detection of GM in serum was observed.
Immunology Service of Infectious Diseases (ISID), Federal University of Bahia, Salvador, Bahia, Brazil, kellypedroza@hotmail.com.
Kelly C M C
KC
Pedroza
Sócrates B
SB
de Matos
Daniel L
DL
de Moura
Mônica B B
MB
Oliveira
Marco A S
MA
Araújo
Roberto J M
RJ
Nascimento
Fernanda W M
FW
Lima
D001228:Aspergillosis / Q000175:diagnosis*; D005690:Galactose / Q000031:analogs & derivatives; D006801:Humans; D007124:Immunoenzyme Techniques / Q000379:methods*; D008351:Mannans / Q000097:blood*; D015203:Reproducibility of Results; D044967:Serum / Q000737:chemistry*
D008351:Mannans; C012990:galactomannan; D005690:Galactose