[The combined effects of beta-sheet breaker and hUCMSC on APP transgenic mice].
OBJECTIVE
To study the effect of combining the injection of beta-sheet breaker H102 with human umbilical cord mesenchymal stem cell (hUCMSC) on APP transgenic mice behavior, P-tau, apoptosis and the expression of relevant enzymes in the brain.


METHODS
APP transgenic mice were randomly divided into model group, hUCMSC group, H102 group, H102 with hUCMSC group and a group of C57BL/6J mice with the same age and background was set as normal. After two weeks and four weeks, the ability of spatial reference memory was tested by Morris Water Maze. After four weeks, immunohistochemical stain and Western blot were done to detect the content of Bad, Bax, Bcl-2, Bcl-xl, P-tau, GSK-3beta, PP-2A and PP-1 in mice brain.


RESULTS
The ability of memory of hUCMSC in 2 weeks group was slightly improved than that in the model group. hUCMSC in four weeks group, H102 group and H102 with hUCMSC group significantly improved the ability of and memory, and reduced the phosphorylation of tau and brain cell's apoptosis of the Alzheimer disease (AD) mice.


CONCLUSIONS
Beta-sheet breaker H102 together with transplanting hUCMSC is an effective therapeutic strategy for AD.
Department of Physiology, Tianjin Medical University, Tianjin 300070, China.
Feng-Xian
FX
Sun
Man
M
Wang
Yan-Ling
YL
Xu
Lai-Xiang
LX
Lin
Shu-Mei
SM
Xu
D000544:Alzheimer Disease / Q000628:therapy*; D016564:Amyloid beta-Protein Precursor / Q000235:genetics*; D000818:Animals; D004195:Disease Models, Animal; D006801:Humans; D018782:Maze Learning; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D010455:Peptides / Q000627:therapeutic use*; D014470:Umbilical Cord / Q000166:cytology
D016564:Amyloid beta-Protein Precursor; D010455:Peptides