Description of Rhabdias breviensis n. sp. (Rhabditoidea: Rhabdiasidae) in two Neotropical frog species.
Nematodes of the genus Rhabdias Stiles & Hassall, 1905 (Rhabditoidea: Rhabdiasidae) have a dioecious free-living stage and a hermaphroditic stage that parasitises the lungs of amphibians and reptiles. Approximately 94 species of Rhabdias have been described. Because the similar morphological characteristics such as the labial structures, the location of the vulva and the shape of the tail of Rhabdias spp. hinder their identification, molecular biology techniques and scanning electron microscopy have been employed to diagnose species of this genus. This study describes Rhabdias breviensis n. sp., parasitic in the lungs of two Neotropical frog species Leptodactylus petersii Steindachner and Leptodactylus macrosternum Miranda-Ribeiro. The description of this species integrates classical taxonomy, scanning electron microscopy and a molecular analysis of the mitochondrial COI gene. The new species differs from all other Rhabdias species parasitic in Neotropical hosts in certain morphometric parameters, the position of the vulva, the host group and the cephalic characters.
Laboratório de Biologia Celular e Helmintologia Profa. Dra. Reinalda Marisa Lanfredi, Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém 66075-110, Brazil.
Luciana de Cássia Silva
Lde C
do Nascimento
Evonnildo Costa
EC
Gonçalves
Francisco Tiago de Vasconcelos
FT
Melo
Elane Guerreiro
EG
Giese
Adriano Penha
AP
Furtado
Jeannie Nascimento
JN
dos Santos
D000818:Animals; D001001:Anura / Q000469:parasitology*; D001483:Base Sequence; D001938:Brazil; D018509:DNA, Helminth / Q000737:chemistry / Q000235:genetics; D005260:Female; D050259:Genes, Mitochondrial / Q000235:genetics; D008168:Lung / Q000469:parasitology; D008855:Microscopy, Electron, Scanning / Q000662:veterinary; D008969:Molecular Sequence Data; D017196:Rhabditida Infections / Q000469:parasitology*; D017172:Rhabditoidea / Q000033:anatomy & histology / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D017422:Sequence Analysis, DNA / Q000662:veterinary
D018509:DNA, Helminth