Epithelial expression of extracellular matrix metalloproteinase inducer/CD147 and matrix metalloproteinase-2 in neoplasms and precursor lesions derived from cutaneous squamous cells: An immunohistochemical study.
Extracellular matrix metalloproteinase inducer (CD147) is a transmembrane glycoprotein involved in the regulation of matrix metalloproteinases (MMPs). The study investigated CD147 and MMP-2 expression in epidermis of cutaneous squamous lesions. CD147 and MMP-2 expressions were evaluated immunohistochemically in 44 specimens: 18 actinic keratoses (AK), 6 squamous cell carcinomas in situ (SCCIS), 13 squamous cell carcinomas (SCC; peritumoral and invasive portions assessed), and 7 normal skins. Patterns of expression were assessed, with MMP-2 in nuclei (MMP-2n) and cytoplasm (MMP-2c) evaluated separately. The expression of each marker was quantified using a calculated immunohistochemical/histologic score (H-score). Correlations were analyzed for the marker H-scores in each study group. Associations between H-scores and histopathologic parameters were also evaluated. CD147 H-score was the highest in SCC (invasive islands), followed by AK, SCCIS, and control specimens, respectively. MMP-2n and MMP-2c H-scores were the highest in AK, followed by SCCIS, SCC, and control specimens, respectively. MMP-2c and MMP-2n H-scores were significantly higher in peritumoral epidermis than in invasive islands of SCC. MMP-2c and CD147 H-scores were positively correlated in the peritumoral SCCs. CD147 H-score was positively correlated with tumor differentiation in SCC. The findings suggest that overexpression of CD147 plays a role in the development of SCC.
Department of Pathology, Baskent University School of Medicine, 79. sok 7/4 Bahcelievler, 06490 Ankara, Turkey. Electronic address: ekupana@yahoo.com.
Sebnem Kupana
SK
Ayva
Ayse Anil
AA
Karabulut
Ayşe Nur
AN
Akatli
Pinar
P
Atasoy
Onder
O
Bozdogan
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D051926:Basigin / Q000032:analysis / Q000096:biosynthesis*; D014408:Biomarkers, Tumor / Q000032:analysis; D002294:Carcinoma, Squamous Cell / Q000378:metabolism*; D004847:Epithelial Cells / Q000378:metabolism*; D005110:Extracellular Space / Q000201:enzymology; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D055623:Keratosis, Actinic / Q000378:metabolism; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000032:analysis / Q000096:biosynthesis*; D008875:Middle Aged; D011230:Precancerous Conditions / Q000378:metabolism*; D012878:Skin Neoplasms / Q000378:metabolism*
C497886:BSG protein, human; D014408:Biomarkers, Tumor; D051926:Basigin; D020778:Matrix Metalloproteinase 2
Actinic keratosis; Basigin; CD147; Cutaneous squamous cell carcinoma; Metalloproteinases