CD40 signal regulates CXCR4 mediating ovarian carcinoma cell migration: implications for extrapelvic metastastic factors.
Ovarian carcinomas are highly invasive, especially in the peritoneal cavity. SDF-1α and its receptor, CXCR4, play a crucial role in migration of cancer cells. Here, SDF-1α directed HO8910 cell migration, but not SKOV3 cells. After being educated to express CXCR4 in vivo or by treating with sCD40L, SDF-1α reexhibited the ability of directing SKOV3 cell migration, which could be antagonized by CXCR4-neutralizing antibody. Furthermore, concomitant expression of CXCR4/CD40 in ovarian carcinoma tissues had stronger correlation with pelvic metastasis than did each alone. It is suggest that SDF-1α acts through CXCR4 to induce ovarian cancer cell migration, which could be facilitated by CD40 activation. Simultaneously examining the expression of CXCR4 and CD40 will provide valuable diagnosis of pelvic metastasis for ovarian carcinomas.
Clinical Immunology Institute, The First Affiliated Hospital of Soochow University, Suzhou, China.
Qiu-Xia
QX
Qu
Qin
Q
Huang
Jian
J
Xu
Li-Ting
LT
Duan
Yi-Bei
YB
Zhu
Xue-Guang
XG
Zhang
D000818:Animals; D014408:Biomarkers, Tumor; D019013:CD40 Antigens / Q000032:analysis / Q000502:physiology*; D002277:Carcinoma / Q000737:chemistry / Q000473:pathology* / Q000556:secondary; D045744:Cell Line, Tumor / Q000378:metabolism / Q000473:pathology / Q000637:transplantation; D002465:Cell Movement; D054377:Chemokine CXCL12 / Q000502:physiology*; D018920:Coculture Techniques; D005260:Female; D006801:Humans; D008207:Lymphatic Metastasis; D051379:Mice; D008819:Mice, Nude; D018613:Microscopy, Confocal; D009362:Neoplasm Metastasis / Q000503:physiopathology*; D009363:Neoplasm Proteins / Q000032:analysis / Q000502:physiology*; D010051:Ovarian Neoplasms / Q000737:chemistry / Q000473:pathology*; D010386:Pelvic Neoplasms / Q000737:chemistry / Q000556:secondary; D012333:RNA, Messenger / Q000235:genetics; D012334:RNA, Neoplasm / Q000235:genetics; D019718:Receptors, CXCR4 / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*
D014408:Biomarkers, Tumor; D019013:CD40 Antigens; C515255:CXCL12 protein, human; C527276:CXCR4 protein, human; D054377:Chemokine CXCL12; D009363:Neoplasm Proteins; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D019718:Receptors, CXCR4