Muscle-specific substrate use during cycle exercise at 1 G: implications for astronaut muscle health.
BACKGROUND
Studies of real and simulated microgravity exposure show the lower limb muscles atrophy to the greatest extent, with the calf muscles being most affected and most difficult to target with exercise countermeasures. This ground-based study examined the metabolic involvement of the thigh and calf muscles during two cycle exercise protocols (moderate and high intensity) central to the exercise countermeasures program on the International Space Station.


METHODS
Intramuscular glycogen and triglyceride levels were quantified in the vastus lateralis and soleus muscles before and after a moderate (current ISS prescription: 45 min at 55% VO(2max), 131 +/- 12 W) and high (proposed ISS prescription: 8 x 30-s intervals at 150% VO(2max), 459 +/- 34 W) intensity cycle exercise bout in nine individuals.


RESULTS
During moderate intensity cycling, glycogen was significantly reduced in the vastus lateralis (114 +/- 27 mmol x kg(-1) dry weight) and remained unchanged in the soleus. High intensity cycling significantly reduced glycogen in both muscles, but the vastus lateralis (151 +/- 25 mmol x kg(-1) dry weight) used significantly more (-160%) than the soleus (59 +/- 11 mmol x kg(-1) dry weight). Intramuscular triglycerides were unchanged in both muscles at both intensities.


CONCLUSIONS
These findings, coupled with other ground-based studies, provide strong support for high intensity cycling being a more appropriate component of the ISS prescription for upper and lower leg skeletal muscle health and cardiorespiratory fitness, although additional exercise paradigms that target the calf are warranted. These muscle-specific findings should be considered when designing exercise strategies for combating conditions of sarcopenia and muscle wasting on Earth.
Human Performance Laboratory, Ball State University, Muncie, IN 47306, USA.
Bridget E
BE
Lester
Robert A
RA
Standley
Jonah D
JD
Lee
William J
WJ
Fink
Scott W
SW
Trappe
Todd A
TA
Trappe
D015094:3-Hydroxyacyl CoA Dehydrogenases / Q000378:metabolism; D000328:Adult; D000337:Aerospace Medicine; D001834:Body Water / Q000378:metabolism; D002950:Citrate (si)-Synthase / Q000378:metabolism; D005080:Exercise Test; D005260:Female; D006003:Glycogen / Q000378:metabolism; D024981:Glycogen Phosphorylase / Q000378:metabolism; D006339:Heart Rate / Q000502:physiology; D006801:Humans; D035002:Lower Extremity / Q000502:physiology*; D008297:Male; D018482:Muscle, Skeletal / Q000378:metabolism*; D010101:Oxygen Consumption / Q000502:physiology; D005082:Physical Exertion / Q000502:physiology; D014280:Triglycerides / Q000378:metabolism; D014893:Weightlessness
D014280:Triglycerides; D006003:Glycogen; D015094:3-Hydroxyacyl CoA Dehydrogenases; D002950:Citrate (si)-Synthase; D024981:Glycogen Phosphorylase