A ferrofluid-based homogeneous assay for highly sensitive and selective detection of single-nucleotide polymorphisms.
A simple and low-cost colorimetric assay utilizing ferrofluidic nanoparticulate probes (FNPs) and a ligase for single-nucleotide polymorphism genotyping is described. Excellent sensitivity and selectivity were accomplished through the engagement of the FNPs and a ligase chain reaction.
Department of Chemistry, National University of Singapore, Singapore.
Wei
W
Shen
Cai Le
CL
Lim
Zhiqiang
Z
Gao
D001681:Biological Assay / Q000379:methods*; D003124:Colorimetry / Q000191:economics / Q000379:methods; D008025:Ligases / Q000737:chemistry; D057230:Limit of Detection; D008280:Magnetics; D053758:Nanoparticles / Q000737:chemistry; D020641:Polymorphism, Single Nucleotide
D008025:Ligases