Zip1, Zip2, and Zip8 mRNA expressions were associated with growth hormone level during the growth hormone provocation test in children with short stature.
Short stature of children is affected by multiple factors. One of them is growth hormone (GH) deficiency. Growth hormone therapy can increase the final height of children with growth hormone deficiency. Zinc is found to induce dimerization and to enhance the bioactivity of human GH. Two gene families have been identified involved in zinc homeostasis. Previous studies in our laboratory have shown that Zip1, Zip2, Zip6, and ZnT1 mRNA were associated with zinc level in established human breast cancer in nude mice model; Zip8 was significantly lower in zinc-deficient Wistar rats in kidney. In this study, five zinc transporters: Zip1, Zip2, Zip6, Zip8, and ZnT1 were chosen. We aimed to investigate the mRNA expression of zinc transporters and to explore the relationship between zinc transporters and growth hormone in short stature children. Growth hormone provocation test is used to confirm the diagnosis of growth hormone deficiency. Six short children for the test were enrolled. At the same time, 15 sex- and age-matched normal children were enrolled as control. The expression levels of zinc transporters in peripheral blood mononuclear cells were determined by quantitative real-time PCR. Zip1 and Zip2 mRNA expression positively correlated with growth hormone level (r = 0.5133, P = 0.0371; r = 0.6719, P = 0.0032); Zip8 mRNA expression negatively correlated with growth hormone level (r = -0.5264, P = 0.0285) during the test in short stature children. The average expression level of Zip2 was significantly higher and Zip6, Zip8 mRNA levels were significantly lower in short stature children than in health controls at 0 min (P < 0.05, P < 0.05).
Department of Biochemistry and Molecular Biology, Shandong University School of Medicine, Jinan, Shandong, China.
Ping
P
Sun
Shifu
S
Wang
Yali
Y
Jiang
Yanting
Y
Tao
Yuanyuan
Y
Tian
Kai
K
Zhu
Haiyan
H
Wan
Lehai
L
Zhang
Lianying
L
Zhang
D027682:Cation Transport Proteins / Q000235:genetics*; D002648:Child; D002675:Child, Preschool; D005260:Female; D015870:Gene Expression; D006130:Growth Disorders / Q000097:blood / Q000175:diagnosis / Q000235:genetics*; D013006:Growth Hormone / Q000097:blood* / Q000172:deficiency; D006801:Humans; D007118:Immunoassay; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D008163:Luminescent Measurements; D008297:Male; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015032:Zinc / Q000097:blood
D027682:Cation Transport Proteins; D012333:RNA, Messenger; C498299:SLC30A1 protein, human; C403517:SLC39A1 protein, human; C498424:SLC39A2 protein, human; C530151:SLC39A8 protein, human; D013006:Growth Hormone; D015032:Zinc