In vitro activity of fosfomycin against a collection of clinical Pseudomonas aeruginosa isolates from 16 Spanish hospitals: establishing the validity of standard broth microdilution as susceptibility testing method.
The broth microdilution method for fosfomycin and Pseudomonas aeruginosa was assessed and compared with the approved agar dilution method in 206 genetically unrelated P. aeruginosa clinical isolates. Essential agreement between the two methods was 84%, and categorical agreement was 89.3%. Additionally, Etest and disk diffusion assays were performed. Results validate broth microdilution as a reliable susceptibility testing method for fosfomycin against P. aeruginosa. Conversely, unacceptable concordance was established between Etest and disk diffusion results with agar dilution results.
Servicio de Microbiología, Hospital Universitario Ramón y Cajal, and Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS), Madrid, Spain.
María
M
Díez-Aguilar
María-Isabel
MI
Morosini
Rosa
R
del Campo
María
M
García-Castillo
Javier
J
Zamora
Rafael
R
Cantón
D000362:Agar; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D003470:Culture Media; D005578:Fosfomycin / Q000494:pharmacology*; D006801:Humans; D016014:Linear Models; D008826:Microbial Sensitivity Tests; D011552:Pseudomonas Infections / Q000188:drug therapy / Q000382:microbiology; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000254:growth & development / Q000302:isolation & purification
D000900:Anti-Bacterial Agents; D003470:Culture Media; D005578:Fosfomycin; D000362:Agar