Oxidative stress in paracetamol-induced pathogenesis: (I). Renal damage.
The effect of administration of paracetamol (1 g/kg body wt) on oxidative damage to proteins and lipids in the kidney was studied at various time intervals in adult male Wistar rats. Iindicators of oxidative stress, such as protein thiol, protein carbonyl content and lipid peroxide levels were assayed along with thiol-dependent enzyme activities, glutamine synthase and glyceraldehyde-3-phosphate dehydrogenase. Paracetamol-induced renal damage after 4 hr of administration was evidenced by elevation in plasma creatinine levels and the presence of acute tubular necrosis on histological examination of the kidney. No significant change in any other parameters was observed, except for decreased glutathione level. An increase in lipid peroxide level was observed at 24 hr after treatment. The results suggest that oxidative stress may not play a causative role, but contribute to the pathogenesis of paracetamol-induced renal damage.
Department of Biochemistry, Christian Medical College, Bagayam, Vellore 632 002, Tamil Nadu, India. premilaabraham@yahoo.com
Premila
P
Abraham
D000082:Acetaminophen / Q000633:toxicity*; D058186:Acute Kidney Injury / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology*; D018712:Analgesics, Non-Narcotic / Q000633:toxicity*; D000818:Animals; D003404:Creatinine / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008054:Lipid Peroxides / Q000378:metabolism; D008297:Male; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000187:drug effects*; D050050:Protein Carbonylation / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar
D018712:Analgesics, Non-Narcotic; D008054:Lipid Peroxides; D000082:Acetaminophen; D003404:Creatinine; D005978:Glutathione