MicroRNA-10b promotes the migration of mouse bone marrow-derived mesenchymal stem cells and downregulates the expression of E-cadherin.
The ability of mesenchymal stem cells (MSCs) to migrate is an important determinant of the efficiency of MSC transplant therapy. MicroRNA-10b (miR-10b) has been positively involved in the migration of a number of tumor cells lineages. To date, it remains unknown whether miR-10b affects the migration of MSCs. In the current study, the effect of miR-10b on the migration of mouse bone marrow-derived MSCs (bmMSCs) was investigated. Third-passage bmMSCs were transfected with miR-10b mimic and negative control precursor miRNA using Lipofectamine™ 2000. miR-10b and E-cadherin expression and bmMSC migration were determined. The present results showed that primary bmMSCs exhibit a spindled or triangular morphology and that third‑passage bmMSCs present a typical fibroblast-like morphology, exhibiting CD90-positive and CD45-negative expression. Compared with the transfection of negative control miRNA, transfection of miR-10b mimic markedly upregulated miR-10b expression in bmMSCs, increased their migration and downregulated E-cadherin expression. The current observations indicate that the upregulation of miR-10b increases bmMSC migration ability, which may be involved in the downregulation of E-cadherin.
Department of Anatomy, Sanquan College, Xinxiang Medical University, Xinxiang, Henan 453003, P.R. China. fxzhang0824@gmail.com
Fenxi
F
Zhang
Suhua
S
Jing
Tongming
T
Ren
Juntang
J
Lin
D000818:Animals; D064200:Cdh1 Proteins / Q000235:genetics* / Q000378:metabolism; D002465:Cell Movement; D048430:Cell Shape; D002478:Cells, Cultured; D015536:Down-Regulation; D017493:Leukocyte Common Antigens / Q000378:metabolism; D059630:Mesenchymal Stem Cells / Q000502:physiology*; D051379:Mice; D035683:MicroRNAs / Q000502:physiology*; D034622:RNA Interference
D064200:Cdh1 Proteins; C577585:Fzr1 protein, mouse; C569259:MIRN10 microRNA, mouse; D035683:MicroRNAs; D017493:Leukocyte Common Antigens; C516770:Ptprc protein, mouse