Zero effect of Bt rice on expression of genes coding for digestion, detoxification and immune responses and developmental performances of brown planthopper Nilaparvata lugens (Stål).
Transgenic Cry1Ac, Cry2Aa and Cry1Ca (Bt toxins) rice lines are well developed to manage lepidopteron pests in China. The impact of transgenic Bt rice on the non-target Brown Planthopper (BPH) has become an essential part of environmental risk assessment, however, scanty evidence is found addressing on developmental and molecular responses of BPH to the ingestion of Bt protein from transgenic rice. The focus of the current study is to examine the developmental characteristics and the expression profiles of gene in relation to digestion, detoxification and immune responses were examined. Our study strongly revealed that the tested Bt rice strains have no unfavorable effect on fecundity, survival and growth of BPH. Furthermore, each of the tested genes did not exhibit distinct expression pattern responding to non Bt parental cultivar, thus, it could be concluded that Bt rice have no detrimental effects on the physiological processes of digestion, detoxification and immune responses of BPH.
Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, Huazhong Agricultural University, Wuhan, 430070 Hubei, China; Department of Agricultural Biology, Faculty of Agriculture, University of Ruhuna, Kamburupitiya 81100, Sri Lanka.
Amani
A
Mannakkara
Lin
L
Niu
Weihua
W
Ma
Chaoliang
C
Lei
D000818:Animals; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000633:toxicity*; D004063:Digestion / Q000187:drug effects; D004731:Endotoxins / Q000633:toxicity*; D005260:Female; D015870:Gene Expression / Q000187:drug effects*; D006430:Hemiptera / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D006460:Hemolysin Proteins / Q000633:toxicity*; D007109:Immunity / Q000187:drug effects; D008658:Inactivation, Metabolic; D008297:Male; D012275:Oryza; D030821:Plants, Genetically Modified
D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D004731:Endotoxins; D006460:Hemolysin Proteins; C030956:insecticidal crystal protein, Bacillus Thuringiensis
Gene expression; Insect performance; Nilaparvata lugens; Quantitative real-time PCR; Transgenic Bt rice