Simultaneous determination of four bioactive compounds in Glechoma longituba extracts by high performance liquid chromatography.
BACKGROUND
Glechoma longituba, one of the long-practiced traditional Chinese medicines in history, is still commonly used nowadays in oriental countries. Previous study indicates that phenolic acid and flavonoids have considerable bioactivities, thus, chlorogenic acid, caffeic acid, apigenin-7-O-glucoside and rosmarinic acid were chosen as the marker components for the simultaneous determination to evaluate the intrinsic quality of G. longituba, and related high performance liquid chromatographic method was urgent to established.


METHODS
A HPLC method was established for simultaneous determination of four major active components in G. longituba, three batches which collected from different suppliers in Zhejiang, Anhui and Jiangsu Provinces were determined.


RESULTS
The contents of chlorogenic acid, caffeic acid, apigenin-7-O-glucoside and rosmarinic acid in G. longituba samples analyzed were 0.00225-0.00234%, 0.0238-0.0242%, 0.00271-0.00313%, 0.830-0.896%, respectively.


CONCLUSIONS
The developed method can be applied to the intrinsic quality control of G. longituba.
Engineering Center of State Ministry of Education for Standardization of Chinese Medicine Processing, Nanjing University of Chinese Medicine, Nanjing, China ; Research Center of TCM Processing Technology, Zhejiang Chinese Medical University, Hangzhou, P. R. China.
Qiyuan
Q
Shan
Gang
G
Cao
Hao
H
Cai
Baochang
B
Cai
Glechoma longituba; High performance liquid chromatography; quality control; simultaneous determination