Decrease of miR-202-3p expression, a novel tumor suppressor, in gastric cancer.
Emerging studies have indicated that microRNAs are involved in the development and progression of cancer. Here we found that miR-202-3p was frequently down-regulated in gastric cancer tissues. Overexpression of miR-202-3p in gastric cancer cells MKN-28 and BGC-823, markedly suppressed cell proliferation and induced cell apoptosis both in vitro and in vivo. Furthermore, Gli1 expression was frequently positive in gastric cancer tissues and inversely correlated with miR-133b expression. We demonstrate that the transcriptional factor Gli1 was a target of miR-202-3p and plays an essential role as a mediator of the biological effects of miR-202-3p in gastric cancer. MiR-202-3p also inhibited the expression of γ-catenin and BCL-2. Taken together, these findings suggest that miR-202-3p may function as a novel tumor suppressor in gastric cancer and its anti-tumor activity may attribute the direct targeting and inhibition of Gli1.
Shanghai Key Laboratory of Gastric Neoplasms, Department of Surgery, Shanghai Institute of Digestive Surgery, Ruijin Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.
Yu
Y
Zhao
Chenglong
C
Li
Ming
M
Wang
Liping
L
Su
Ying
Y
Qu
Jianfang
J
Li
Beiqin
B
Yu
Min
M
Yan
Yingyan
Y
Yu
Bingya
B
Liu
Zhenggang
Z
Zhu
D000368:Aged; D000818:Animals; D017209:Apoptosis; D002277:Carcinoma / Q000235:genetics* / Q000378:metabolism / Q000473:pathology / Q000601:surgery; D045744:Cell Line, Tumor; D005260:Female; D005743:Gastrectomy; D015972:Gene Expression Regulation, Neoplastic; D005822:Genetic Vectors; D006801:Humans; D008297:Male; D051379:Mice; D008819:Mice, Nude; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D008875:Middle Aged; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D012190:Retroviridae / Q000235:genetics; D013274:Stomach Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology / Q000601:surgery; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism; D047368:Tumor Burden; D000071676:Zinc Finger Protein GLI1; D051185:gamma Catenin / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism
C400551:GLI1 protein, human; C548234:MIRN133 microRNA, human; C569392:MIRN202 microRNA, human; D035683:MicroRNAs; D019253:Proto-Oncogene Proteins c-bcl-2; D014157:Transcription Factors; D000071676:Zinc Finger Protein GLI1; D051185:gamma Catenin