Application of DNA microarray technology to gerontological studies.
Gene expression patterns change dramatically in aging and age-related events. The DNA microarray is now recognized as a useful device in molecular biology and widely used to identify the molecular mechanisms of aging and the biological effects of drugs for therapeutic purpose in age-related diseases. Recently, numerous technological advantages have led to the evolution of DNA microarrays and microarray-based techniques, revealing the genomic modification and all transcriptional activity. Here, we show the step-by-step methods currently used in our lab to handling the oligonucleotide microarray and miRNA microarray. Moreover, we introduce the protocols of ribonucleoprotein [RNP] immunoprecipitation followed by microarray analysis (RIP-chip) which reveal the target mRNA of age-related RNA-binding proteins.
Department of Stress Science, Institute of Health Biosciences, The University of Tokushima Graduate School, Tokushima, Japan.
Kiyoshi
K
Masuda
Yuki
Y
Kuwano
Kensei
K
Nishida
Kazuhito
K
Rokutan
D000375:Aging / Q000235:genetics*; D015870:Gene Expression / Q000235:genetics; D006801:Humans; D047468:Immunoprecipitation / Q000379:methods; D035683:MicroRNAs / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D016601:RNA-Binding Proteins / Q000235:genetics; D012261:Ribonucleoproteins / Q000032:analysis / Q000378:metabolism
D035683:MicroRNAs; D016601:RNA-Binding Proteins; D012261:Ribonucleoproteins