Application of microsatellite primers developed for Polistes in the independent-founding wasp Polists satan Bequaert (Hymenoptera: Vespidae).
Microsatellite primers developed for a given species are sometimes useful for another in the same genus and in other genera within the same family, making possible to search for pre-existing suitable primers in the databanks such as GenBank. We examined whether existing primers developed for Polistes could be used for Polistes satan Bequaert. We tested 50 microsatellite primers from three Polistes species and found that six microsatellite loci show polymorphism in size in P. satan. These six loci were highly polymorphic, having four to 15 alleles in P. satan with an expected heterozygosity of 0.525-0.832. These loci can be used to study parameters concerning genetic relatedness such as social interactions in colonies and genetic conflicts of interest among nestmate individuals.
Lab of Insect Ecology, Fac of Education, Niigata Univ, Niigata, Japan.
K
K
Komatsu
S
S
Mateus
R
R
Zucchi
F
F
Nascimento
K
K
Kudô
D000818:Animals; D017931:DNA Primers; D005260:Female; D008297:Male; D018895:Microsatellite Repeats / Q000235:genetics*; D014863:Wasps / Q000235:genetics*
D017931:DNA Primers