[Study on effects and mechanism of traditional Mongolian medicine wuweifengshi capsule on adjuvant arthritis in rats].
OBJECTIVE
To study the effects and immunoregulation mechanism of the traditional Mongolian medicine Wuweifengshi capsule on adjuvant arthritis (AA).


METHODS
Wister rats were divided into several groups: normal group, AA model group, Wuweifengshi capsule groups (with low, moderate, high dose of 0.2, 0.4, 0.8 g x kg(-1) x d(-1) respectively), and Zhonglun-5 group (original dose of 1.68 g x kg(-1) x d(-1)). The edema degree, the level of IL-1beta, TNF-alpha, PGE2, NO and MDA and the activity of SOD in serum were detected. Through cell culture, the effects of the medicine on AA rat's splenic cell's multiplication capacity were studied. The influence of celiac macrophage cell culture fluid of AA rats' on C57BL/6J mice thymic cell multiplication capacity under the medicine was evaluated.


RESULTS
Wuweifengshi capsule showed an inhibiting function on the level of IL-1beta, TNF-alpha, PGE2, NO and increased the activity of SOD in serum, but showed no significant influence on MDA. It also inhibited the AA rat's splenic cell's multiplication capacity and the influence of celiac macrophage cell culture fluid of AA rat's on C57BL/6J mice thymic cell multiplication capacity.


CONCLUSIONS
The anti-AA effect of Wuweifengshi capsule is possibly due to its inhibition of relevant cytokines and its adjustment of corresponding enzyme's activity and immunization organ's cell multiplication capacity.
College of Mongolian Medicine and Pharmacy, Inner Mongolian University of Nationality, Tongliao 028300, China. tlmdwxl@163.com
Xiu-Lan
XL
Wang
Wan-Xue
WX
Huo
Ren-Jie
RJ
Lu
Serguleng
S
Han
Chun-Xia
CX
Bao
D000818:Animals; D001169:Arthritis, Experimental / Q000188:drug therapy* / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D002214:Capsules; D003683:Dehydroascorbic Acid / Q000031:analogs & derivatives / Q000097:blood; D015232:Dinoprostone / Q000378:metabolism; D004195:Disease Models, Animal; D004487:Edema / Q000188:drug therapy; D005260:Female; D053583:Interleukin-1beta / Q000378:metabolism; D008214:Lymphocytes / Q000276:immunology / Q000378:metabolism; D017737:Macrophages, Peritoneal / Q000378:metabolism; D008297:Male; D056785:Medicine, Mongolian Traditional; D051379:Mice; D009569:Nitric Oxide / Q000378:metabolism; D051381:Rats; D013154:Spleen / Q000166:cytology / Q000378:metabolism; D013482:Superoxide Dismutase / Q000097:blood; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D002214:Capsules; D053583:Interleukin-1beta; D014409:Tumor Necrosis Factor-alpha; D009569:Nitric Oxide; C000820:semidehydroascorbic acid; D013482:Superoxide Dismutase; D015232:Dinoprostone; D003683:Dehydroascorbic Acid