Modulation of Pleurodeles waltl DNA polymerase mu expression by extreme conditions encountered during spaceflight.
DNA polymerase µ is involved in DNA repair, V(D)J recombination and likely somatic hypermutation of immunoglobulin genes. Our previous studies demonstrated that spaceflight conditions affect immunoglobulin gene expression and somatic hypermutation frequency. Consequently, we questioned whether Polμ expression could also be affected. To address this question, we characterized Polμ of the Iberian ribbed newt Pleurodeles waltl and exposed embryos of that species to spaceflight conditions or to environmental modifications corresponding to those encountered in the International Space Station. We noted a robust expression of Polμ mRNA during early ontogenesis and in the testis, suggesting that Polμ is involved in genomic stability. Full-length Polμ transcripts are 8-9 times more abundant in P. waltl than in humans and mice, thereby providing an explanation for the somatic hypermutation predilection of G and C bases in amphibians. Polμ transcription decreases after 10 days of development in space and radiation seem primarily involved in this down-regulation. However, space radiation, alone or in combination with a perturbation of the circadian rhythm, did not affect Polμ protein levels and did not induce protein oxidation, showing the limited impact of radiation encountered during a 10-day stay in the International Space Station.
Stress Immunity Pathogens Laboratory, EA7300, Lorraine University, Vandœuvre-lès-Nancy, France.
Véronique
V
Schenten
Nathan
N
Guéguinou
Sarah
S
Baatout
Jean-Pol
JP
Frippiat
D000818:Animals; D015153:Blotting, Western; D002940:Circadian Rhythm; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D004259:DNA-Directed DNA Polymerase / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental / Q000528:radiation effects; D015971:Gene Expression Regulation, Enzymologic / Q000528:radiation effects; D007814:Larva / Q000201:enzymology / Q000235:genetics / Q000528:radiation effects; D008297:Male; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction / Q000528:radiation effects; D010802:Phylogeny; D010999:Pleurodeles / Q000196:embryology / Q000235:genetics* / Q000254:growth & development; D050050:Protein Carbonylation / Q000528:radiation effects; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D013026:Space Flight; D013737:Testis / Q000196:embryology / Q000254:growth & development / Q000378:metabolism; D059467:Transcriptome / Q000235:genetics* / Q000528:radiation effects
D018076:DNA, Complementary; C407743:DNA polymerase mu; D004259:DNA-Directed DNA Polymerase