iTRAQ-based quantitative proteomic analysis reveals new metabolic pathways of wheat seedling growth under hydrogen peroxide stress.
As an abundant ROS, hydrogen peroxide (H2 O2 ) plays pivotal roles in plant growth and development. In this work, we conducted for the first time an iTRAQ-based quantitative proteomic analysis of wheat seedling growth under different exogenous H2 O2 treatments. The growth of seedlings and roots was significantly restrained by increased H2 O2 concentration stress. Malondialdehyde, soluble sugar, and proline contents as well as peroxidase activity increased with increasing H2 O2 levels. A total of 3,425 proteins were identified by iTRAQ, of which 157 showed differential expression and 44 were newly identified H2 O2 -responsive proteins. H2 O2 -responsive proteins were mainly involved in stress/defense/detoxification, signal transduction, and carbohydrate metabolism. It is clear that up-regulated expression of signal transduction and stress/defence/detoxification-related proteins under H2 O2 stress, such as plasma membrane intrinsic protein 1, fasciclin-like arabinogalactan protein, and superoxide dismutase, could contribute to H2 O2 tolerance of wheat seedlings. Increased gluconeogenesis (phosphoenol-pyruvate carboxykinase) and decreased pyruvate kinase proteins are potentially related to the higher H2 O2 tolerance of wheat seedlings. A metabolic pathway of wheat seedling growth under H2 O2 stress is presented.
College of Life Sciences, Capital Normal University, Beijing, China.
Pei
P
Ge
Pengchao
P
Hao
Min
M
Cao
Guangfang
G
Guo
Dongwen
D
Lv
Saminathan
S
Subburaj
Xiaohui
X
Li
Xing
X
Yan
Jitian
J
Xiao
Wujun
W
Ma
Yueming
Y
Yan
D016000:Cluster Analysis; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D006861:Hydrogen Peroxide / Q000633:toxicity*; D007553:Isotope Labeling / Q000379:methods*; D053858:Metabolic Networks and Pathways / Q000187:drug effects*; D018515:Plant Leaves / Q000187:drug effects / Q000378:metabolism; D010940:Plant Proteins / Q000145:classification / Q000378:metabolism; D018517:Plant Roots / Q000187:drug effects / Q000378:metabolism; D020543:Proteome / Q000378:metabolism; D040901:Proteomics / Q000379:methods*; D036226:Seedlings / Q000187:drug effects / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D013312:Stress, Physiological / Q000187:drug effects* / Q000235:genetics; D014158:Transcription, Genetic / Q000187:drug effects; D014908:Triticum / Q000187:drug effects / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*
D010940:Plant Proteins; D020543:Proteome; D006861:Hydrogen Peroxide
H2O2 stress; Metabolic network; Plant proteomics; Seedlings; Wheat; iTRAQ