Cell-selective metabolic labeling of biomolecules with bioorthogonal functionalities.
Metabolic labeling of biomolecules with bioorthogonal functionalities enables visualization, enrichment, and analysis of the biomolecules of interest in their physiological environments. This versatile strategy has found utility in probing various classes of biomolecules in a broad range of biological processes. On the other hand, metabolic labeling is nonselective with respect to cell type, which imposes limitations for studies performed in complex biological systems. Herein, we review the recent methodological developments aiming to endow metabolic labeling strategies with cell-type selectivity. The cell-selective metabolic labeling strategies have emerged from protein and glycan labeling. We envision that these strategies can be readily extended to labeling of other classes of biomolecules.
Beijing National Laboratory for Molecular Sciences, Department of Chemical Biology, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China.
Ran
R
Xie
Senlian
S
Hong
Xing
X
Chen
D002470:Cell Survival; D006801:Humans; D050356:Lipid Metabolism; D011134:Polysaccharides / Q000378:metabolism; D011506:Proteins / Q000378:metabolism*; D013379:Substrate Specificity
D011134:Polysaccharides; D011506:Proteins