Oriented covalent immobilization of enzymes on heterofunctional-glyoxyl supports.
Novel heterofunctional glyoxyl-agarose supports were prepared. These supports contained the maximal concentration of glyoxyl groups to promote maximization of covalent immobilization and groups' capability to adsorb proteins by various mechanisms (e.g., ionic exchange, metal-chelate formation). Immobilization on various supports makes it possible to orientate and rigidify an enzyme in various regions of its surface. The use of different heterofunctional supports allowed for obtaining catalysts with different activity, stability, and selectivity properties.
Institute of Catalysis, CSIC, CAMPUS UAM-Cantoblanco, Madrid, Spain.
Cesar
C
Mateo
Gloria
G
Fernandez-Lorente
Javier
J
Rocha-Martin
Juan M
JM
Bolivar
Jose M
JM
Guisan
D000327:Adsorption; D000818:Animals; D001426:Bacterial Proteins / Q000737:chemistry; D002265:Carboxylic Ester Hydrolases / Q000737:chemistry; D002918:Chymotrypsin / Q000737:chemistry; D003432:Cross-Linking Reagents / Q000737:chemistry*; D057075:Enzyme Assays; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry*; D006038:Glyoxylates / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D008049:Lipase / Q000737:chemistry; D012685:Sepharose / Q000737:chemistry*; D013237:Stereoisomerism; D013379:Substrate Specificity
D001426:Bacterial Proteins; D003432:Cross-Linking Reagents; D004800:Enzymes, Immobilized; D006038:Glyoxylates; C070399:glyoxyl agarose; D012685:Sepharose; D002265:Carboxylic Ester Hydrolases; C022711:tannase; D008049:Lipase; D002918:Chymotrypsin