Initial characterization of histone H3 serine 10 O-acetylation.
In eukaryotic organisms, histone posttranslational modifications (PTMs) are indispensable for their role in maintaining cellular physiology, often through their mediation of chromatin-related processes such as transcription. Targeted investigations of this ever expanding network of chemical moieties continue to reveal genetic, biochemical, and cellular nuances of this complex landscape. In this study, we present our findings on a novel class of histone PTMs: Serine, Threonine, and Tyrosine O-acetylation. We have combined highly sensitive nano-LC-MS/MS experiments and immunodetection assays to identify and validate these unique marks found only on histone H3. Mass spectrometry experiments have determined that several of these O-acetylation marks are conserved in many species, ranging from yeast to human. Additionally, our investigations reveal that histone H3 serine 10 acetylation (H3S10ac) is potentially linked to cell cycle progression and cellular pluripotency. Here, we provide a glimpse into the functional implications of this H3-specific histone mark, which may be of high value for further studies of chromatin.
Department of Molecular Biology; Princeton University; Princeton, NJ USA; Epigenetics Program; Department of Biochemistry and Biophysics; Perelman School of Medicine; University of Pennsylvania; Philadelphia, PA USA.
Laura-Mae P
LM
Britton
Molecular and Cellular Oncogenesis Program; The Wistar Institute; Philadelphia, PA USA.
Alyshia
A
Newhart
Epigenetics Program; Department of Biochemistry and Biophysics; Perelman School of Medicine; University of Pennsylvania; Philadelphia, PA USA.
Natarajan V
NV
Bhanu
University of California Los Angeles; David Geffen School of Medicine; Department of Biological Chemistry; Jonsson Comprehensive Cancer Center; Molecular Biology Institute; Bioinformatics Interdepartmental Degree Program; Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research; Los Angeles, CA USA; Current affiliation: Wisconsin Institute for Discovery; Department of Cell and Regenerative Biology; University of Wisconsin; Madison, WI USA.
Rupa
R
Sridharan
Department of Molecular Biology; Princeton University; Princeton, NJ USA; Epigenetics Program; Department of Biochemistry and Biophysics; Perelman School of Medicine; University of Pennsylvania; Philadelphia, PA USA.
Michelle
M
Gonzales-Cope
University of California Los Angeles; David Geffen School of Medicine; Department of Biological Chemistry; Jonsson Comprehensive Cancer Center; Molecular Biology Institute; Bioinformatics Interdepartmental Degree Program; Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research; Los Angeles, CA USA.
Kathrin
K
Plath
Molecular and Cellular Oncogenesis Program; The Wistar Institute; Philadelphia, PA USA.
Susan M
SM
Janicki
Epigenetics Program; Department of Biochemistry and Biophysics; Perelman School of Medicine; University of Pennsylvania; Philadelphia, PA USA.
Benjamin A
BA
Garcia
D000107:Acetylation; D000818:Animals; D002453:Cell Cycle; D002843:Chromatin / Q000378:metabolism*; D002853:Chromatography, Liquid; D004330:Drosophila / Q000378:metabolism; D053595:Embryonic Stem Cells / Q000378:metabolism; D005260:Female; D057809:HEK293 Cells; D006367:HeLa Cells; D006657:Histones / Q000378:metabolism*; D006801:Humans; D008297:Male; D051379:Mice; D039904:Pluripotent Stem Cells / Q000378:metabolism; D011499:Protein Processing, Post-Translational; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D012694:Serine / Q000378:metabolism*; D013045:Species Specificity; D053719:Tandem Mass Spectrometry; D016808:Tetrahymena thermophila / Q000378:metabolism
D002843:Chromatin; D006657:Histones; D012694:Serine
chromatin; epigenetics; histone; mass spectrometry; post-translational modifications; proteomics; quantitative; stem cells