Analysis of major constituents in Fructus aurantii-Magnolia bark decoction by UPLC-PDA.
A ultraperformance liquid chromatography (UPLC)-photodiode array (PDA) detection method was established for the simultaneous determination of seven constituents in Fructus aurantii-Magnolia bark decoction: naringin, hesperidin, neohesperidin, narirutin, meranzin hydrate, honokiol and magnolol. These were separated in <17 min using a C18 column with gradient elution using (a) acetonitrile, (b) water and (c) acetic acid at a flow rate of 0.3 mL/min, and with a PDA detector. All calibration curves showed good linear regression (r(2) > 0.9992) within the test ranges. The method was validated for speciﬁcity, accuracy, precision and limits of detection. The proposed method was found to be suitable for the quality assessment of the formula.
Laboratory of Ethnopharmacology, Institute of Integrated Traditional Medicine and Western Medicine, Xiangya Hospital, Central South University, No. 87 Xiangya Road, Changsha 410008, Hunan Province, China National Key Clinical Specialist Vocational School of TCM Encephalopathy, Xiangya Hospital, Central South University, Changsha, China.
Zhi-hua
ZH
Xing
Laboratory of Ethnopharmacology, Institute of Integrated Traditional Medicine and Western Medicine, Xiangya Hospital, Central South University, No. 87 Xiangya Road, Changsha 410008, Hunan Province, China National Key Clinical Specialist Vocational School of TCM Encephalopathy, Xiangya Hospital, Central South University, Changsha, China.
Wei-jun
WJ
Peng
Laboratory of Ethnopharmacology, Institute of Integrated Traditional Medicine and Western Medicine, Xiangya Hospital, Central South University, No. 87 Xiangya Road, Changsha 410008, Hunan Province, China National Key Clinical Specialist Vocational School of TCM Encephalopathy, Xiangya Hospital, Central South University, Changsha, China TCM Pharmacogenetics Laboratory, Central South University, Changsha, China huangweidavid@aliyun.com.
Wei
W
Huang
Laboratory of Ethnopharmacology, Institute of Integrated Traditional Medicine and Western Medicine, Xiangya Hospital, Central South University, No. 87 Xiangya Road, Changsha 410008, Hunan Province, China National Key Clinical Specialist Vocational School of TCM Encephalopathy, Xiangya Hospital, Central South University, Changsha, China TCM Pharmacogenetics Laboratory, Central South University, Changsha, China.
Xi
X
Huang
Laboratory of Ethnopharmacology, Institute of Integrated Traditional Medicine and Western Medicine, Xiangya Hospital, Central South University, No. 87 Xiangya Road, Changsha 410008, Hunan Province, China National Key Clinical Specialist Vocational School of TCM Encephalopathy, Xiangya Hospital, Central South University, Changsha, China.
Wei-ping
WP
Liu
D002853:Chromatography, Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000032:analysis*; D031566:Magnolia / Q000737:chemistry*; D015203:Reproducibility of Results
D004365:Drugs, Chinese Herbal