Light-inducible activation of target mRNA translation in mammalian cells.
A genetically encoded optogenetic system was constructed that activates mRNA translation in mammalian cells in response to light. Blue light induces the reconstitution of an RNA binding domain and a translation initiation domain, thereby activating target mRNA translation downstream of the binding sites.
Department of Chemical and Biological Engineering, Rensselaer Polytechnic Institute, Troy, New York 12180, USA.
Jicong
J
Cao
Manish
M
Arha
Chaitanya
C
Sudrik
Lukasz J
LJ
Bugaj
David V
DV
Schaffer
Ravi S
RS
Kane
D001665:Binding Sites / Q000528:radiation effects; D057809:HEK293 Cells; D006801:Humans; D008027:Light; D014176:Protein Biosynthesis / Q000528:radiation effects*; D012333:RNA, Messenger / Q000378:metabolism*
D012333:RNA, Messenger