Role of tumor necrosis factor-α, interferon-γ and Fas-ligand on in vitro nitric oxide activity in the corpus luteum.
Normal reproductive function involves the expression of inflammatory mediators. Regarding the corpus luteum (CL), cytokines promote the cross-talk between immune, vascular and steroidogenic cells, among others. Moreover, TNF, IFNG and FASL were shown to regulate equine CL establishment and regression. We hypothesized that cytokines action on equine CL may be mediated by nitric oxide (NO), through the regulation of endothelial NO synthase (eNOS) expression. TNF increased eNOS mRNA level and NO metabolite (nitrite) production during CL growth. Cytokines combined action (TNF+IFNG+FASL) promoted eNOS protein upregulation in mid-CL and nitrite production in mid and late-CL. However, in late-CL, TNF alone decreased nitrite secretion. These results indicate that in equine CL, cytokines TNF, IFNG and FASL regulate NO activity, via eNOS expression modulation.
CIISA, Faculty of Veterinary Medicine, Technical University of Lisbon, Lisbon, Portugal; Department of Reproductive Immunology and Pathology, Institute of Animal Reproduction and Food Research, Olsztyn, Poland. Electronic address: agalvao@fmv.utl.pt.
A M
AM
Galvão
Anna Z
AZ
Szóstek
Dariusz J
DJ
Skarzynski
Graça M
GM
Ferreira-Dias
D000818:Animals; D002478:Cells, Cultured; D003338:Corpus Luteum / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism*; D053222:Fas Ligand Protein / Q000378:metabolism*; D005260:Female; D006736:Horses; D007371:Interferon-gamma / Q000378:metabolism*; D009569:Nitric Oxide / Q000378:metabolism*; D052250:Nitric Oxide Synthase Type III / Q000235:genetics / Q000378:metabolism; D009573:Nitrites / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*; D015854:Up-Regulation
D053222:Fas Ligand Protein; D009573:Nitrites; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D009569:Nitric Oxide; D007371:Interferon-gamma; D052250:Nitric Oxide Synthase Type III
Corpus luteum; Fas-ligand; IFN-γ; Nitric oxide; TNF-α