Multilayered structure based on poly(N,N-dimethyl-acrylamide-co-3,9-divinyl-2,4,8,10-tetraoxaspiro (5.5) undecane) prepared in a multiphase gelation process.
The study is devoted to the preparation of a multi-membrane gel system based on poly(N,N-dimethyl-acrylamide-co-3,9-divinyl-2,4,8,10-tetraoxaspiro (5.5) undecane) copolymer. The complex supra-structure was realized by layer by layer deposition technique, from the gel-core to the periphery by crosslinking poly(N,N-dimethyl-acrylamide-co-3,9-divinyl-2,4,8,10-tetraoxaspiro (5.5) undecane) (P(DMA-co-U)) with N,N'-methylene-bis-acrylamide and using as well β-cyclodextrin with double purpose as compound for constructing further the edge of the interfacial supramolecular assemblies and to improve the host-guest complexation of further bioactive compounds. Basic copolymer has dual thermo- and pH sensitive character. The multi-membrane 'onion-like' gel system was tested as matrix for embedding Norfloxacin. Hematologic, biochemical, immunologic studies used to determine the biocompatibility of multi-membrane gel structures loaded with drug that directly contact living tissue provided a reasonable assessment of safety as implants. Histopathologic exams did not reveal any disturbances of the normal liver architecture in animals with multi-membrane gel system containing Norfloxacin compared to control group with sterile cotton pellets implant. Good biocompatibility for the implants based on multi-membrane gel structures loaded with drug were achieved from the in vivo tests these suggesting that biomaterials may be suitable for in vivo use as a local drug delivery system.
"Petru Poni" Institute of Macromolecular Chemistry, Grigore Ghica Voda Alley No. 41-A, 700487 IASI, Romania. Electronic address: achiriac1@yahoo.com.
Aurica P
AP
Chiriac
Loredana E
LE
Nita
Manuela T
MT
Nistor
Liliana
L
Tartau
D000178:Acrylamides / Q000737:chemistry*; D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000737:chemistry; D001672:Biocompatible Materials / Q000737:chemistry* / Q000494:pharmacology; D016503:Drug Delivery Systems; D005782:Gels; D008099:Liver / Q000187:drug effects / Q000473:pathology; D009643:Norfloxacin / Q000008:administration & dosage / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar; D047392:beta-Cyclodextrins / Q000737:chemistry*
D000178:Acrylamides; D000900:Anti-Bacterial Agents; D001672:Biocompatible Materials; D005782:Gels; D047392:beta-Cyclodextrins; C429790:poly(N,N-dimethylacrylamide); C021221:N,N'-methylenebisacrylamide; C031215:betadex; D009643:Norfloxacin
In vivo tests; Multi-membrane gel; Norfloxacin; Poly(N,N-dimethyl-acrylamide-co-3,9-divinyl-2,4,8,10-tetraoxaspiro (5.5) undecane); β-cyclodextrin