Synthesis of C-terminal peptide thioesters using Fmoc-based solid-phase peptide chemistry.
This chapter provides two protocols for the solid-phase synthesis of peptide thioesters using N (α) -Fmoc-protected amino acids. The first protocol is based on a so-called safety-catch linker, while the second relies on a backbone amide linker.
IGM, Faculty of Life Sciences, University of Copenhagen, Zealand Pharma, Glostrup, Denmark.
Pernille Tofteng
PT
Shelton
Knud J
KJ
Jensen
D000596:Amino Acids / Q000737:chemistry; D004952:Esters / Q000737:chemistry; D005449:Fluorenes / Q000737:chemistry; D010455:Peptides / Q000138:chemical synthesis* / Q000737:chemistry; D060327:Solid-Phase Synthesis Techniques / Q000379:methods*; D013438:Sulfhydryl Compounds / Q000737:chemistry
D000596:Amino Acids; D004952:Esters; D005449:Fluorenes; D010455:Peptides; D013438:Sulfhydryl Compounds