Entire sequence of the colonization factor coli surface antigen 6-encoding plasmid pCss165 from an enterotoxigenic Escherichia coli clinical isolate.
Coli surface antigen 6 (CS6) is one of the most prevalent colonization factors among enterotoxigenic Escherichia coli (ETEC) isolated in developing countries. Although it is known that CS6 is encoded by a plasmid, there are no reports on the sequence analysis of the CS6-encoding plasmid or genes exhibiting similar behavior to CS6. Here, we report the isolation of the CS6-encoding plasmid, pCss165Kan, from 4266 ΔcssB::kanamycin (Km) and its complete nucleotide sequence. This plasmid consisted of 165,311bp and 222 predicted coding sequences. Remarkably, there were many insertion sequence (IS) elements, which comprised 24.4% of the entire sequence. Virulence-associated genes such as heat-stable enterotoxin, homologues of ATP-binding cassette transporter in enteroaggregative E. coli (EAEC), and ETEC autotransporter A were also present, although the ETEC autotransporter A gene was disrupted by the integration of IS629. We found that 2 transcriptional regulators belonging to the AraC family were not involved in CS6 expression. Interestingly, pCss165 had conjugative transfer genes, as well as 3 toxin-antitoxin systems that potentially exclude other plasmid-free host bacteria. These genes might be involved in the prevalence of CS6 among ETEC isolates.
Research Institute, National Center for Global Health and Medicine, 1-21-1, Toyama, Shinjuku, Tokyo 162-8655, Japan; Graduate School of Comprehensive Human Sciences, University of Tsukuba, 1-1-1, Tennodai, Tsukuba, Ibaraki 305-8577, Japan.
Takeaki
T
Wajima
Subrata
S
Sabui
Shigeyuki
S
Kano
Thandavarayan
T
Ramamurthy
Nabendu Sekhar
NS
Chatterjee
Takashi
T
Hamabata
D018528:ATP-Binding Cassette Transporters / Q000235:genetics; D000942:Antigens, Bacterial / Q000235:genetics*; D004251:DNA Transposable Elements; D054307:Enterotoxigenic Escherichia coli / Q000235:genetics* / Q000302:isolation & purification; D004768:Enterotoxins / Q000235:genetics; D004927:Escherichia coli Infections / Q000382:microbiology; D029968:Escherichia coli Proteins / Q000235:genetics*; D036781:Fimbriae Proteins / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial; D006801:Humans; D007612:Kanamycin / Q000737:chemistry; D016366:Open Reading Frames; D010957:Plasmids; D014158:Transcription, Genetic; D037521:Virulence Factors / Q000235:genetics*
D018528:ATP-Binding Cassette Transporters; D000942:Antigens, Bacterial; C098406:CS6 antigen, E coli; D004251:DNA Transposable Elements; D004768:Enterotoxins; D029968:Escherichia coli Proteins; D037521:Virulence Factors; C022924:colonization factor antigens; D036781:Fimbriae Proteins; D007612:Kanamycin
BLAST; CDSs; CFA; CFs; CS; CS6; Colonization factor; DIG; Enterotoxigenic Escherichia coli; IS; Km and Kan; PBS; PHD; ST; Virulence plasmid; basic local alignment search tool; coding sequences; coli surface antigen; colonization factor antigen; colonization factors; digoxigenin; heat-stable enterotoxin; insertion sequence; kanamycin; phosphate buffered saline; prevent host death