Determination of zeranol and its metabolites in bovine muscle and liver by a chemiluminescence enzyme immunoassay: compared to an ultraperformance liquid chromatography tandem mass spectroscopy method.
A chemiluminescent enzyme immunoassay (CLEIA) was compared to an ultraperformance liquid chromatography tandem mass spectroscopy (UPLC-MS/MS) procedure for the analysis of zeranol and its metabolites in bovine tissue samples. Apparent recoveries from fortified samples by both methods were comparable at 0.5-4.0 µg/kg and a significant correlation was obtained. For CLEIA analysis, hapten mimicking the analyte was first synthesized and conjugated with the carrier protein bovine serum albumin as the immunogen to produce monoclonal antibody. The obtained antibody showed extensive cross-reactivity toward zeranol metabolites (zearalanone). The limit of detection of CLEIA and UPLC-MS/MS was 0.05 µg/kg and 0.5 µg/kg, respectively. Recoveries of both methods for fortified samples were higher than 75.0% with the coefficient of variation less than 15%. These results indicated that the combination of screening with CLEIA and confirmation with UPLC-MS/MS for zeranol and its metabolites would be a reliable method for a large number of bovine samples.
Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, China Agricultural University, Yuan Ming Yuan West Road No.2, Beijing, 100193, China.
Jiang
J
Haiyang
Wang
W
Wenjun
Zhu
Z
Jinghui
Tao
T
Xiaoqi
Li
L
Jiancheng
Xia
X
Xi
Wen
W
Kai
Xu
X
Fei
Wang
W
Zhaopeng
Chen
C
Min
Li
L
Xiangmei
Wu
W
Xiaoping
Wang
W
Shien
Ding
D
Shuangyang
D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D013006:Growth Hormone / Q000032:analysis / Q000378:metabolism; D007124:Immunoenzyme Techniques / Q000379:methods*; D008099:Liver / Q000737:chemistry* / Q000378:metabolism; D008163:Luminescent Measurements / Q000379:methods*; D018482:Muscle, Skeletal / Q000737:chemistry* / Q000378:metabolism; D053719:Tandem Mass Spectrometry / Q000379:methods*; D015029:Zeranol / Q000032:analysis* / Q000378:metabolism
D015029:Zeranol; D013006:Growth Hormone
UPLC-MS/MS; Zeranol (ZAL); bovine muscle and liver; chemiluminescent enzyme immunoassay (CLEIA)