Comparative proteomics of inhaled silver nanoparticles in healthy and allergen provoked mice.
BACKGROUND
Silver nanoparticles (AgNPs) have been associated with the exacerbation of asthma; however, the immunological basis for the adjuvant effects of AgNPs is not well understood.


OBJECTIVE
The aim of the study reported here was to investigate the allergic effects of AgNP inhalation using proteomic approaches.


METHODS
Allergen provoked mice were exposed to 33 nm AgNPs at 3.3 mg/m(3). Following this, bronchoalveolar lavage fluid (BALF) and plasma were collected to determine protein profiles.


RESULTS
In total, 106 and 79 AgNP-unique proteins were identified in the BALF of control and allergic mice, respectively. Additionally, 40 and 26 AgNP-unique proteins were found in the plasma of control and allergic mice, respectively. The BALF and plasma protein profiles suggested that metabolic, cellular, and immune system processes were associated with pulmonary exposure to AgNPs. In addition, we observed 18 proteins associated with systemic lupus erythematosus that were commonly expressed in both control and allergic mice after AgNP exposure. Significant allergy responses were observed after AgNP exposure in control and allergic mice, as determined by ovalbumin-specific immunoglobulin E.


CONCLUSIONS
Inhaled AgNPs may regulate immune responses in the lungs of both control and allergic mice. Our results suggest that immunology is a vital response to AgNPs.
Division of Pulmonary Medicine, Department of Internal Medicine, Shuang Ho Hospital, School of Respiratory Therapy, Taipei Medical University, Taipei, Taiwan.
Chien-Ling
CL
Su
Tzu-Tao
TT
Chen
Chih-Cheng
CC
Chang
Kai-Jen
KJ
Chuang
Cheng-Kuan
CK
Wu
Wen-Te
WT
Liu
Kin Fai
KF
Ho
Kang-Yun
KY
Lee
Shu-Chuan
SC
Ho
Hsiu-Er
HE
Tseng
Hsiao-Chi
HC
Chuang
Tsun-Jen
TJ
Cheng
D000280:Administration, Inhalation; D000485:Allergens / Q000633:toxicity*; D000818:Animals; D001798:Blood Proteins / Q000032:analysis / Q000737:chemistry; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry; D005260:Female; D053768:Metal Nanoparticles / Q000008:administration & dosage / Q000737:chemistry / Q000633:toxicity*; D051379:Mice; D008807:Mice, Inbred BALB C; D010047:Ovalbumin / Q000633:toxicity; D020543:Proteome / Q000032:analysis / Q000737:chemistry / Q000187:drug effects*; D040901:Proteomics; D012834:Silver / Q000008:administration & dosage / Q000737:chemistry / Q000633:toxicity*
D000485:Allergens; D001798:Blood Proteins; D020543:Proteome; D012834:Silver; D010047:Ovalbumin
bronchoalveolar lavage; immunotoxicology; proteome; serum; systemic lupus erythematosus