Enhancement of 4-acetylantroquinonol B production by supplementation of its precursor during submerged fermentation of Antrodia cinnamomea.
The antiproliferation activity of the ethanol extract of A. cinnamomea mycelium on hepatocellular cancer cells HepG2 was found to be associated with aroma intensity of the broth during fermentation. We hypothesized that some of the volatile compounds are the precursors of the key bioactive component 4-acetylantroquinonol B of this fungus. The major volatile compounds of A. cinnamomea were identified by GC/MS, and they are oct-1-en-3-ol, linalool, methyl phenylacetate, nerolidol, γ-cadinene and 2,4,5-trimethoxybenzaldehyde (TMBA). TMBA and nerolidol were further selected and used as supplements during fermentation. It was found that both of them could increase the production of 4-acetylantroquinonol B and enhance the antiproliferation activity of the fungus. In addition, the TMBA was identified as the most promising supplement for increasing the bioactivity of A. cinnamomea during cultivation.
Institute of Food Science and Technology, National Taiwan University , Taipei 106, Taiwan.
Chien-Chi
CC
Chiang
Tzu-Ning
TN
Huang
Yu-Wei
YW
Lin
Kai-Hsien
KH
Chen
Been-Huang
BH
Chiang
D015107:4-Butyrolactone / Q000031:analogs & derivatives* / Q000032:analysis / Q000096:biosynthesis / Q000494:pharmacology; D000970:Antineoplastic Agents / Q000032:analysis / Q000378:metabolism*; D055491:Antrodia / Q000737:chemistry / Q000378:metabolism*; D049109:Cell Proliferation / Q000187:drug effects; D003470:Culture Media / Q000737:chemistry / Q000378:metabolism; D003512:Cyclohexanones / Q000032:analysis / Q000494:pharmacology; D005285:Fermentation; D008401:Gas Chromatography-Mass Spectrometry; D056945:Hep G2 Cells; D006801:Humans
D000970:Antineoplastic Agents; D003470:Culture Media; D003512:Cyclohexanones; C555021:4-acetylantroquinonol B; D015107:4-Butyrolactone