The Upf factor complex interacts with aberrant products derived from mRNAs containing a premature termination codon and facilitates their proteasomal degradation.
Up-frameshift (Upf) factors eliminate aberrant mRNAs containing a specific premature termination codon (PTC). Here, we show that Upf complex facilitates the ubiquitin-dependent degradation of products derived from mRNA containing specific PTCs in Saccharomyces cerevisiae. The efficiency of recruitment of the Upf complex to a PTC product was correlated with the decay of the PTC product. Upf factors promoted the degradation of the human von Hippel-Lindau (VHL) protein, which is an unfolded protein in yeast cells, in a manner that depends on the presence of a faux 3'-UTR. Mass spectrometric analysis and Western blot analysis revealed that Hsp70 was associated with the PTC product. These findings suggest that the Upf complex may be recruited to ribosomes in a faux 3'-UTR-dependent manner and then associates with aberrant products to facilitate their degradation by the proteasome.
From the Graduate School of Pharmaceutical Sciences, Tohoku University, Sendai 980-8578 and.
Kazushige
K
Kuroha
Koji
K
Ando
Reiko
R
Nakagawa
Toshifumi
T
Inada
D020413:3' Untranslated Regions / Q000235:genetics; D018389:Codon, Nonsense / Q000378:metabolism*; D057809:HEK293 Cells; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism; D006801:Humans; D008954:Models, Biological; D046912:Multiprotein Complexes / Q000378:metabolism*; D010735:Phosphoglycerate Kinase / Q000378:metabolism; D046988:Proteasome Endopeptidase Complex / Q000378:metabolism*; D011485:Protein Binding; D058767:Protein Unfolding; D059748:Proteolysis; D012331:RNA, Fungal / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism*; D054875:Ubiquitination; D051794:Von Hippel-Lindau Tumor Suppressor Protein / Q000378:metabolism
D020413:3' Untranslated Regions; D018389:Codon, Nonsense; D018840:HSP70 Heat-Shock Proteins; D046912:Multiprotein Complexes; D012331:RNA, Fungal; D012333:RNA, Messenger; D029701:Saccharomyces cerevisiae Proteins; D051794:Von Hippel-Lindau Tumor Suppressor Protein; D010735:Phosphoglycerate Kinase; D046988:Proteasome Endopeptidase Complex; C497446:VHL protein, human
Gene Regulation; Protein Folding; Protein Turnover; Ribosomes; mRNA Decay