[The migration of CXCR4(+); BMSCs and the ox-LDL-induced injury in endothelial cells].
OBJECTIVE
To study whether the stromal cell-derived factor 1 (SDF-1) secreted by endothelial cells affects the CXCR4(+); stem cell migration.


METHODS
CXCR4(+); bone marrow-derived mesenchymal stem cells (BMSCs) were isolated from mouse bone marrow. Human umbilical vein endothelial cells (HUVECs) were stimulated with oxidized low-density lipoprotein (ox-LDL), and the cell proliferation was detected by MTT assay, the expressions of SDF-1α mRNA and protein were detected by RT-PCR and ELISA, respectively. The migration of CXCR4(+); BMSCs was analyzed by Transwell(R); chamber assay.


RESULTS
The stimulation of ox-LDL affected the proliferation and increased significantly the expression levels of SDF-1α mRNA and protein in HUVECs, and the medium supernatant promoted the migratory response of CXCR4(+); BMSCs. When the neutralizing CXCR4 antibody eliminated the secreted SDF-1α, the migratory activity markedly decreased.


CONCLUSIONS
CXCR4(+); BMSCs might migrate to endothelial cells by SDF-1α/CXCR4 axis in the atherosclerosis process.
School of Basic Medicine, Xianning Central Hospital, Hubei University of Science and Technology, Xianning, China. yugang428@yahoo.com.cn
Gang
G
Yu
Hongli
H
Xia
Mincai
M
Li
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000378:metabolism / Q000502:physiology; D002465:Cell Movement / Q000235:genetics / Q000502:physiology*; D002478:Cells, Cultured; D054377:Chemokine CXCL12 / Q000235:genetics / Q000378:metabolism; D042783:Endothelial Cells / Q000166:cytology* / Q000378:metabolism*; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D008077:Lipoproteins, LDL / Q000235:genetics / Q000378:metabolism*; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism / Q000502:physiology; D051379:Mice; D008810:Mice, Inbred C57BL; D019718:Receptors, CXCR4 / Q000235:genetics / Q000378:metabolism*
D054377:Chemokine CXCL12; D008077:Lipoproteins, LDL; D019718:Receptors, CXCR4; C099252:oxidized low density lipoprotein