An improved protocol for electroporation in members of the genus Gordonia.
Gordonia are high GC gram-positive bacteria that have not yet been exploited well for biotechnological purposes because of the limited genetic tools. Described here is an improved protocol for electroporation, which is useful for several Gordonia species. The maximum transformation efficiency obtained was 2.8 × 10(4)/μg (Gordonia rubropertinctus, Gordonia sp), and 1.7 × 10(3)/μg (Gordonia amarae).
Department of Biochemical Engineering and Biotechnology, Indian Institute of Technology, Delhi, Hauz Khas, New Delhi 110016, India.
Pooja
P
Singh
Preeti
P
Srivastava
D001709:Biotechnology; D018274:Electroporation / Q000379:methods*; D033601:Gordonia Bacterium / Q000235:genetics*; D010957:Plasmids / Q000235:genetics
Gordonia; Plasmid pRSG43; Transformation