Effect of Lutein on L-NAME-Induced Hypertensive Rats.
We investigated the antihypertensive effect of lutein on N(G) -nitro-L-arginine methyl ester hydrochloride (L-NAME)-induced hypertensive rats. Daily oral administration of L-NAME (40 mg/kg)-induced a rapid progressive increase in mean arterial pressure (MAP). L-NAME significantly increased MAP from the first week compared to that in the control and reached 193.3±9.6 mmHg at the end of treatment. MAP in the lutein groups was dose-dependently lower than that in the L-NAME group. Similar results were observed for systolic and diastolic blood pressure of L-NAME-induced hypertensive rats. The control group showed little change in heart rate for 3 weeks, whereas L-NAME significantly reduced heart rate from 434±26 to 376±33 beats/min. Lutein (2 mg/kg) significantly prevented the reduced heart rate induced by L-NAME. L-NAME caused hypertrophy of heart and kidney, and increased plasma lipid peroxidation four-fold but significantly reduced plasma nitrite and glutathione concentrations, which were significantly prevented by lutein in a dose-dependent manner. These findings suggest that lutein affords significant antihypertensive and antioxidant effects against L-NAME-induced hypertension in rats.
College of Pharmacy, Chung-Ang University, Seoul 156-756, Korea.
Ji Hoon
JH
Sung
Young Soo
YS
Jo
Su Jin
SJ
Kim
Jeong Soo
JS
Ryu
Myung Chul
MC
Kim
Hyun Ju
HJ
Ko
Sang Soo
SS
Sim
Antioxidant; Hypertension; L-NAME; Lipid peroxidation; Lutein