[Dynamic effect of curcumin on urine concentration of neuronal thread protein in AD mice].
OBJECTIVE
Through the dynamic detection of the concentration change of the urine Alzheimer-associated neuronal thread protein (AD7C-NTP) in the curcumin treated Alzheimer's disease (AD) model (APP/PS1 double transgenic) mice, the therapeutic effect of curcumin in AD was determined.


METHODS
Thirty three-month-old APP /PS1 double transgenic mice were randomly divided into 5 groups, 6 in each group, the model group, rosiglitazone group(10 mg . kg-1 . d-1) , high(400 mg . kg -1 . d-1) , medium(200 mg . kg-1. d-1) and low(100 mg . kg-1 . d-1) dose curcumin groups. Six C57BL/6J mice in the same age and genetic background were used as normal control group. All the 6 groups of mice were intragastrically administered for 6 months. Urine samples were collected on 4 month, 5 month and 6 month after intragastric administration, respectively. The changes of urinary AD7C-NTP concentration were detected by enzyme-linked immunosorbent assay (ELISA).


RESULTS
The concentration of AD7C-NTP of each group was compared at the same time point, the concentration of model group is higher than normal control group (P <0.05) ; the concentration of other groups is lower than model group. The concentration of high curcumin dose group with 4 months treatment, has no statistical difference compared with model group. The AD7C-NTP concentration of each group was elevated with the age growth, and all concentrations of the treatment groups were lower than the model group at the same period. With the treatment of 4, 5 and 6 months, the concentration of the normal control group has significant difference with the treatment groups(P <0. 01). There have no statistical difference between all the groups with the treatment of 6 months compared with 5 months.


CONCLUSIONS
With the progression of the disease in AD mice, there are fluctuations in urinary AD7C-NTP concentration, the compound curcumin from traditional Chinese medicine can delay the progression of AD.
Key Laboratory of Pharmacology of Dongzhimen Hospital, Beijing University of Chinese Medicine, State Administration of Traditional Chinese Medicine, Bei4ing 100700, China. pingqingsong15@163.com
Xiao-Pei
XP
Chen
Rui-Sheng
RS
Li
Hong
H
Wang
Ying
Y
Ren
Hai-Yun
HY
Sun
Jin-Duo
JD
Yang
Peng-Wen
PW
Wang
D000544:Alzheimer Disease / Q000188:drug therapy* / Q000652:urine*; D000818:Animals; D003474:Curcumin / Q000627:therapeutic use*; D004797:Enzyme-Linked Immunosorbent Assay; D051379:Mice; D008822:Mice, Transgenic; D009419:Nerve Tissue Proteins / Q000652:urine*; D000077154:Rosiglitazone; D045162:Thiazolidinediones / Q000627:therapeutic use
C109804:AD7c-NTP; D009419:Nerve Tissue Proteins; D045162:Thiazolidinediones; D000077154:Rosiglitazone; D003474:Curcumin