Molecular characterization of a duck Tembusu virus from China.
A new emerging flavivirus caused severe egg-drop in poultry and spread quickly across most duck-producing regions of China in 2010. Complete genome sequencing indicated that the virus genome is 10,989 nucleotides in length and possesses typical flavivirus genome organization, 5' untranslated region (UTR)-Cv-Ci-prM-M-E-NS1-NS2A-NS2B-NS3-NS4A-2K-NS4B-NS5-3'-UTR. The long open reading frame (ORF) encodes 3,425 amino acids (95-10,372 nt). The 94-nucleotide 5'-UTR is of intermediate size and the 617-nucleotide 3'-UTR is quite long relative to those of other flaviviruses. The polyprotein cleavage sites, potential glycosylation sites, distribution of cysteine residues, and 3'-UTR secondary structure were characterized. Phylogenetic analysis of the polyprotein sequences indicates that the HN isolate is closely related to Tembusu viruses of the Ntaya virus group.
State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, No. 427 Maduan Street, Harbin, 150001, People's Republic of China.
XiaoFei
X
Bai
Rang
R
Lv
ChunGuo
C
Liu
Na
N
Qiu
Yilong
Y
He
XiuChen
X
Yin
XiaoJun
X
Li
Ming
M
Liu
Yun
Y
Zhang
D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D000818:Animals; D002681:China; D004372:Ducks; D005416:Flavivirus / Q000737:chemistry / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D018177:Flavivirus Infections / Q000662:veterinary* / Q000821:virology; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D010802:Phylogeny; D011201:Poultry Diseases / Q000821:virology*; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics
D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D012367:RNA, Viral; D014764:Viral Proteins