Evaluation of the presence of VEGF, BMP2 and CBFA1 proteins in autogenous bone graft: histometric and immunohistochemical analysis.
OBJECTIVE
The purpose of this study was to evaluate the expression of proteins that participate in the osteoinduction stage (VEGF, BMP2 and CBFA1) of the process of bone regeneration of defects created in rat calvariae and filled with autogenous bone block grafts.


METHODS
10 adult male rats (Rattus norvegicus albinus, Wistar) were used, who received two bone defects measuring 5 mm each in the calvariae. The bone defects constituted two experimental groups (n = 10): Control Group (CONT) (defects filled with a coagulum); Graft Group (GR) (defects filled with autogenous bone removed from the contralateral defect). The animals were submitted to euthanasia at 7 and 30 days post-operatively.


RESULTS
Quantitative analysis demonstrated significantly greater bone formation in Group GR, but the presence of the studied proteins was significantly greater in the CONT Group in both time intervals of observation.


CONCLUSIONS
It was not possible in this study in cortical bone block groups to detect the osteoinductive proteins in a significant amount during the repair process.
Dental School of Araçatuba, University of State of São Paulo "Júlio de Mesquita Filho" - UNESP, Rua José Bonifácio, 1193, CEP 16015-050 Araçatuba, SP, Brazil. Electronic address: mhguskuma@gmail.com.
Marcos Heidy
MH
Guskuma
Dental School of Araçatuba, University of State of São Paulo "Júlio de Mesquita Filho" - UNESP, Rua José Bonifácio, 1193, CEP 16015-050 Araçatuba, SP, Brazil.
Eduardo
E
Hochuli-Vieira
Dental School of Araçatuba, University of State of São Paulo "Júlio de Mesquita Filho" - UNESP, Rua José Bonifácio, 1193, CEP 16015-050 Araçatuba, SP, Brazil.
Flávia Priscila
FP
Pereira
Dental School of Araçatuba, University of State of São Paulo "Júlio de Mesquita Filho" - UNESP, Rua José Bonifácio, 1193, CEP 16015-050 Araçatuba, SP, Brazil.
Idelmo
I
Rangel-Garcia
Dental School of Araçatuba, University of State of São Paulo "Júlio de Mesquita Filho" - UNESP, Rua José Bonifácio, 1193, CEP 16015-050 Araçatuba, SP, Brazil.
Roberta
R
Okamoto
Dental School of Araçatuba, University of State of São Paulo "Júlio de Mesquita Filho" - UNESP, Rua José Bonifácio, 1193, CEP 16015-050 Araçatuba, SP, Brazil.
Tetuo
T
Okamoto
Dental School of Araçatuba, University of State of São Paulo "Júlio de Mesquita Filho" - UNESP, Rua José Bonifácio, 1193, CEP 16015-050 Araçatuba, SP, Brazil.
Osvaldo Magro
OM
Filho
D000818:Animals; D064592:Autografts / Q000737:chemistry* / Q000473:pathology / Q000637:transplantation; D001777:Blood Coagulation / Q000502:physiology; D001857:Bone Matrix / Q000737:chemistry / Q000473:pathology; D055396:Bone Morphogenetic Protein 2 / Q000032:analysis*; D001861:Bone Regeneration / Q000502:physiology; D016025:Bone Transplantation / Q000379:methods; D001842:Bone and Bones / Q000737:chemistry* / Q000473:pathology; D050659:Core Binding Factor Alpha 1 Subunit / Q000032:analysis*; D042783:Endothelial Cells / Q000737:chemistry / Q000473:pathology; D007091:Image Processing, Computer-Assisted / Q000379:methods; D007150:Immunohistochemistry; D008297:Male; D008853:Microscopy / Q000379:methods; D018919:Neovascularization, Physiologic / Q000502:physiology; D010006:Osteoblasts / Q000737:chemistry / Q000473:pathology; D010012:Osteogenesis / Q000502:physiology; D010294:Parietal Bone / Q000601:surgery; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors; D042461:Vascular Endothelial Growth Factor A / Q000032:analysis*
C525720:Bmp2 protein, rat; D055396:Bone Morphogenetic Protein 2; D050659:Core Binding Factor Alpha 1 Subunit; C493717:Runx2 protein, rat; D042461:Vascular Endothelial Growth Factor A; C467498:vascular endothelial growth factor A, rat
Bone Morphogenetic Protein 2; Bone transplant; Core Binding Factor Alpha 1 Subunit; Immunohistochemistry; Vascular Endothelial Growth Factor A