In vitro anti-oxidative activities and GC-MS analysis of various solvent extracts of Cassia singueana parts.
The present study was conducted to investigate the anti-oxidative activities of different solvent extracts of Cassia singueana parts. Our results indicate that all the extracts have reducing power (Fe3+ --> Fe2+) and DPPH radical scavenging abilities. However, the ethyl acetate extract of the stem bark has the highest total reducing power whilst the ethanol extract of the stem bark has more potent free radical scavenging activity than all the other extracts. The ethyl acetate extract of the stem bark exhibited more powerful hydroxyl radical scavenging activity than other extracts whilst the aqueous extract of the leaves displayed more potent nitric oxide inhibition activity than other extracts. The GC-MS analysis of the ethyl acetate extract of the stem bark and the ethanol extract of the root and leaves indicated that several aromatic compounds, including phenolics, fatty acids, amino acids and triterpenoids were present in these extracts. Data from this study suggest that the parts of C. singueana possessed anti-oxidative activities and can be used as a potential alternative medicine for oxidative stress related non-communicable chronic diseases. Further experimental and clinical studies in this regard are warranted.
School of Life Sciences, University of KwaZulu-Natal (Westville Campus), Durban 4000, South Africa.
Mohammed Auwal
MA
Ibrahim
Neil Anthony
NA
Koorbanally
Md Shahidul
MS
Islam
D000085:Acetates / Q000737:chemistry; D000975:Antioxidants / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D001713:Biphenyl Compounds / Q000737:chemistry; D002366:Cassia / Q000737:chemistry*; D002712:Chlorides / Q000737:chemistry; D000431:Ethanol / Q000737:chemistry; D005290:Ferric Compounds / Q000737:chemistry; D008401:Gas Chromatography-Mass Spectrometry; D017665:Hydroxyl Radical / Q000737:chemistry; D009569:Nitric Oxide / Q000737:chemistry; D010851:Picrates / Q000737:chemistry; D024301:Plant Bark; D010936:Plant Extracts / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D018515:Plant Leaves; D018517:Plant Roots; D010946:Plants, Medicinal; D012997:Solvents / Q000737:chemistry*; D014867:Water / Q000737:chemistry
D000085:Acetates; D000975:Antioxidants; D001713:Biphenyl Compounds; D002712:Chlorides; D005290:Ferric Compounds; D010851:Picrates; D010936:Plant Extracts; D012997:Solvents; D014867:Water; D009569:Nitric Oxide; D017665:Hydroxyl Radical; D000431:Ethanol; C007650:ethyl acetate; C004931:1,1-diphenyl-2-picrylhydrazyl; C024555:ferric chloride