Flavonoids from Tetrastigma obtectum enhancing glucose consumption in insulin-resistance HepG2 cells via activating AMPK.
Four new flavonoids including three C-glycosidic flavonoids, named tetrastigma A-D (1-4) and five known flavones (5-9) were isolated from the herbs of Tetrastigma obtectum (Wall.) Planch. The structures of 1-4 were elucidated by 1D- and 2D-NMR and HR mass spectrometric methods. Compound 1 caused significant enhancement in glucose consumption by insulin-resistant HepG2 cells compared with control cells. In addition, compound 1 stimulated phosphorylation of AMPK, which plays an important role in glycometabolism.
Tianjin Key Laboratory on Technologies Enabling Development Clinical Therapeutics and Diagnostics (Theranostics), School of Pharmacy, Tianjin Medical University, Tianjin 300070, People's Republic of China.
Mei-Na
MN
Jin
Guo-Ru
GR
Shi
Sheng-An
SA
Tang
Nan-Qin
Wei
W
Qiao
Hong-Quan
HQ
Duan
D055372:AMP-Activated Protein Kinases / Q000378:metabolism; D005419:Flavonoids / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D005947:Glucose / Q000378:metabolism*; D056945:Hep G2 Cells; D006801:Humans; D007004:Hypoglycemic Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D007333:Insulin Resistance; D015394:Molecular Structure; D010766:Phosphorylation; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D027844:Vitaceae / Q000737:chemistry*
D005419:Flavonoids; D007004:Hypoglycemic Agents; D010936:Plant Extracts; D055372:AMP-Activated Protein Kinases; D005947:Glucose
AMPK; Flavonoid; HepG2 glucose consumption; Tetrastigma obtectum