Measuring the orientation of taurine in the active site of the non-heme Fe(II)/α-ketoglutarate-dependent taurine hydroxylase (TauD) using electron spin echo envelope modulation (ESEEM) spectroscopy.
The position and orientation of taurine near the non-heme Fe(II) center of the α-ketoglutarate (α-KG)-dependent taurine hydroxylase (TauD) was measured using Electron Spin Echo Envelope Modulation (ESEEM) spectroscopy. TauD solutions containing Fe(II), α-KG, and natural abundance taurine or specifically deuterated taurine were prepared anaerobically and treated with nitric oxide (NO) to make an S = 3/2 {FeNO}(7) complex that is suitable for robust analysis with EPR spectroscopy. Using ratios of ESEEM spectra collected for TauD samples having natural abundance taurine or deuterated taurine, (1)H and (14)N modulations were filtered out of the spectra and interactions with specific deuterons on taurine could be studied separately. The Hamiltonian parameters used to calculate the amplitudes and line shapes of frequency spectra containing isolated deuterium ESEEM were obtained with global optimization algorithms. Additional statistical analysis was performed to validate the interpretation of the optimized parameters. The strongest (2)H hyperfine coupling was to a deuteron on the C1 position of taurine and was characterized by an effective dipolar distance of 3.90 ± 0.25 Å from the {FeNO}(7) paramagnetic center. The principal axes of this C1-(2)H hyperfine coupling and nuclear quadrupole interaction tensors were found to make angles of 26 ± 5 and 52 ± 17°, respectively, with the principal axis of the {FeNO}(7) zero-field splitting tensor. These results are discussed within the context of the orientation of substrate taurine prior to the initiation of hydrogen abstraction.
Department of Chemistry, ‡Department of Microbiology and Molecular Genetics, and Department of Biochemistry and Molecular Biology, Michigan State University , East Lansing, Michigan, 48824.
Thomas M
TM
Casey
Piotr K
PK
Grzyska
Robert P
RP
Hausinger
John
J
McCracken
D000465:Algorithms; D055162:Biocatalysis; D020134:Catalytic Domain; D056831:Coordination Complexes / Q000737:chemistry; D003903:Deuterium / Q000737:chemistry; D004578:Electron Spin Resonance Spectroscopy; D005296:Ferrous Compounds / Q000737:chemistry; D007656:Ketoglutaric Acids / Q000737:chemistry; D006899:Mixed Function Oxygenases / Q000737:chemistry* / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism; D013654:Taurine / Q000737:chemistry* / Q000378:metabolism
D056831:Coordination Complexes; D005296:Ferrous Compounds; D007656:Ketoglutaric Acids; D013654:Taurine; D009569:Nitric Oxide; D003903:Deuterium; D006899:Mixed Function Oxygenases; C433064:taurine-alpha-ketoglutarate dioxygenase