[Optimization of extraction process for Baizhu Shaoyao San by multi-index orthogonal experiment].
Baizhu Shaoyao San is a famous traditional Chinese medicine formula, which is efficient to diarrhea clinically. However, there are very few reports for extraction process of Baizhu Shaoyao San at present. In this article, an orthogonal test was conducted, with 10 major components and extractum of Baizhu Shaoyao San as indexes, and data was processed by the compound weight method, in order to detect four factors, including the concentration of ethanol, the dosage of ethanol, the soaking and extracting times, and optimize the extraction process of Baizhu Shaoyao San. The results showed that the optimal extraction process was adding 12-fold ethanol of 70% concentration, soaking for 1 hours, and extracting for twice, 2 hours each time. Among the indexes, the extracting time made significant impact. The optimized method is stable and reliable, and can provide reference for further development and utilization of the formula.
Engineering Center for Chinese Medicine Processing under State Ministry of Education, Nanjing University of Chinese Medicine, Nanjing 210029, China.
Li-Juan
LJ
Zheng
Kun-Ming
KM
Qin
Hao
H
Cai
Gang
G
Cao
Bao-Chang
BC
Cai
D005591:Chemical Fractionation / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000032:analysis / Q000302:isolation & purification*; D010946:Plants, Medicinal / Q000737:chemistry*
D004365:Drugs, Chinese Herbal