Different types of aqueous two-phase systems for biomolecule and bioparticle extraction and purification.
Upstream improvements have led to significant advances in the productivity of biomolecules and bioparticles. Today, downstream processes are the bottleneck in the production of some biopharmaceuticals, a change from previous years. Current purification platforms will reach their physical limits at some point, indicating the need for new approaches. This article reviews an alternative method to extract and purify biomolecules/bioparticles named aqueous two-phase system (ATPS). Biocompatibility and readiness to scale up are some of the ATPS characteristics. We also discuss some of ATPS applications in the biotechnology field.
Dept. of Biochemical and Pharmaceutical Technology, Faculty of Pharmaceutical Sciences, University of São Paulo, Av. Prof. Lineu Prestes, 580, Block 16, Cidade Universitária, São Paulo, 05508-000, Brazil.
João Vitor Dutra
JV
Molino
Daniela de Araújo
Dde A
Viana Marques
Adalberto Pessoa
AP
Júnior
Priscila Gava
PG
Mazzola
Maria Silvia Viccari
MS
Gatti
D001702:Biopharmaceutics; D001709:Biotechnology; D006801:Humans; D011506:Proteins / Q000737:chemistry / Q000302:isolation & purification*; D014867:Water / Q000737:chemistry*
D011506:Proteins; D014867:Water
aqueous two-phase system; biomolecules; bioparticles; liquid-liquid extraction