Tip-enhanced Raman spectroscopy for the base interrogation of DNA.
A combination of scanning probe and Raman microscopies allows for the label-free chemical mapping of surfaces with spatial resolutions one to two orders of magnitude below the diffraction limit. Because Raman spectroscopy provides a highly specific molecular "fingerprint," the resulting spectra allow speciation of even highly similar chemical compounds. The surface enhancement phenomenon allows Raman signals from a species to be enhanced by many orders of magnitude within the vicinity (~10nm) of a source of free electrons such as a noble metal. Presenting the metal surface as a single highly localized point, and moving this point with angstrom accuracy across a distributed, heterogenous surface while collecting Raman spectra, is what is known as tip-enhanced Raman spectroscopy, or TERS.
Radiation Monitoring Devices, Watertown, Massachusetts, USA.
Noah
N
Kolodziejski
D001483:Base Sequence; D004247:DNA / Q000235:genetics* / Q000648:ultrastructure; D018736:DNA Adducts / Q000235:genetics / Q000648:ultrastructure; D006801:Humans; D018625:Microscopy, Atomic Force / Q000295:instrumentation / Q000379:methods; D010084:Oxidation-Reduction; D017422:Sequence Analysis, DNA / Q000295:instrumentation / Q000379:methods*; D013059:Spectrum Analysis, Raman / Q000295:instrumentation / Q000379:methods
D018736:DNA Adducts; D004247:DNA
DNA oxidation; SERS; Single-molecule sequencing; Super-resolution; TERS; Tip-enhanced Raman spectroscopy