A new omics data resource of Pleurocybella porrigens for gene discovery.
BACKGROUND
Pleurocybellaporrigens is a mushroom-forming fungus, which has been consumed as a traditional food in Japan. In 2004, 55 people were poisoned by eating the mushroom and 17 people among them died of acute encephalopathy. Since then, the Japanese government has been alerting Japanese people to take precautions against eating the P. porrigens mushroom. Unfortunately, despite efforts, the molecular mechanism of the encephalopathy remains elusive. The genome and transcriptome sequence data of P. porrigens and the related species, however, are not stored in the public database. To gain the omics data in P. porrigens, we sequenced genome and transcriptome of its fruiting bodies and mycelia by next generation sequencing.


RESULTS
Short read sequences of genomic DNAs and mRNAs in P. porrigens were generated by Illumina Genome Analyzer. Genome short reads were de novo assembled into scaffolds using Velvet. Comparisons of genome signatures among Agaricales showed that P. porrigens has a unique genome signature. Transcriptome sequences were assembled into contigs (unigenes). Biological functions of unigenes were predicted by Gene Ontology and KEGG pathway analyses. The majority of unigenes would be novel genes without significant counterparts in the public omics databases.


CONCLUSIONS
Functional analyses of unigenes present the existence of numerous novel genes in the basidiomycetes division. The results mean that the omics information such as genome, transcriptome and metabolome in basidiomycetes is short in the current databases. The large-scale omics information on P. porrigens, provided from this research, will give a new data resource for gene discovery in basidiomycetes.
Graduate School of Science and Technology, Shizuoka University, Suruga-ku, Shizuoka, Japan.
Tomohiro
T
Suzuki
Kaori
K
Igarashi
Hideo
H
Dohra
Takumi
T
Someya
Tomoyuki
T
Takano
Kiyonori
K
Harada
Saori
S
Omae
Hirofumi
H
Hirai
Kentaro
K
Yano
Hirokazu
H
Kawagishi
D000363:Agaricales / Q000235:genetics*; D001203:Ascomycota / Q000235:genetics; D004271:DNA, Fungal / Q000235:genetics; D030541:Databases, Genetic; D048690:Fruiting Bodies, Fungal / Q000235:genetics; D015966:Gene Expression Regulation, Fungal; D056726:Genetic Association Studies; D016681:Genome, Fungal / Q000235:genetics; D023281:Genomics; D006801:Humans; D053858:Metabolic Networks and Pathways / Q000235:genetics; D058977:Molecular Sequence Annotation; D025282:Mycelium / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017422:Sequence Analysis, DNA; D017423:Sequence Analysis, RNA; D013045:Species Specificity
D004271:DNA, Fungal; D012333:RNA, Messenger