Fabrication of an electrochemical biosensor array for simultaneous detection of L-glutamate and acetylcholine.
Using two nano-porous pseudo carbon paste electrodes (Nano-PPCPEs) as the working electrodes, a saturated calomel electrode as the reference electrode, and a Pt electrode as the counter electrode to form a four-electrode system, a novel type of glutamate-acetylcholine electrochemical biosensor array was successfully fabricated. It was shown that the biosensor array displayed a high selectivity and sensitivity and the selective and simultaneous determination of glutamate and acetylcholine (ATCh) is feasible. For L-glutamate, a linear range from 5 x 10(-7) M to 1 x 10(-5) M with the detection limit of 2.5 x 10(-7) M was demonstrated, while for ATChl the linear range is from 5 x 10(-6) M to 2 x 10(-4) M with the detection limit of 1.5 x 10(-7) M. This research means a possibility to simultaneously detect more neurotransmitters with a nano-PPCPEs electrochemical biosensor array.
Key Laboratory of Green Packaging and Application of Biological Nanotechnology, Hunan University of Technology, Zhuzhou 412007, China. dy_dengyan@yahoo.com.cn
Yan
Y
Deng
Wei
W
Wang
Chao
C
Ma
Zhiyang
Z
Li
D000109:Acetylcholine / Q000032:analysis*; D015374:Biosensing Techniques / Q000295:instrumentation*; D002244:Carbon / Q000737:chemistry; D055664:Electrochemical Techniques / Q000295:instrumentation* / Q000379:methods; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000378:metabolism; D005240:Feasibility Studies; D018698:Glutamic Acid / Q000032:analysis*; D057230:Limit of Detection; D008855:Microscopy, Electron, Scanning; D055616:Microtechnology / Q000379:methods; D049329:Nanostructures / Q000737:chemistry; D010088:Oxidoreductases / Q000378:metabolism; D016062:Porosity; D040081:Protein Array Analysis / Q000295:instrumentation*; D012680:Sensitivity and Specificity
D004800:Enzymes, Immobilized; D018698:Glutamic Acid; D002244:Carbon; D010088:Oxidoreductases; D000109:Acetylcholine