Enhanced activity of demineralised bone matrix augmented with xenogeneic bone morphogenetic protein complex in rats.
BACKGROUND
Demineralised bone matrix (DBM) is an allograft material widely used as a bone filler and bone graft substitute. DBM contains bone morphogenetic proteins (BMPs), which induce and regulate bone formation during embryogenesis and in postnatal life.


OBJECTIVE
To investigate the osteoinductivity of DBM augmented with xenogeneic BMP-complex at different doses.


METHODS
Rat DBM was augmented with BMP-complex purified from porcine diaphyseal bone.


RESULTS
Dorsal subcutaneous implantation of 25 mg rat allogeneic DBM augmented with 0, 3, 6 and 12 mg BMP-complex per gram of DBM resulted in dose dependant up-regulation of bone formation on day 21, as scored histologically and biochemically.


CONCLUSIONS
Allogeneic DBM can be augmented with xenogeneically sourced BMP-complex to improve DBM performance in vivo. This work demonstrates the potential of BMP-complex augmented DBM to induce new bone formation with improved parameters of bone formation.
Department of Oral Medicine and Periodontology, School of Oral Health Sciences, University of the Witwatersrand, South Africa. mohangi@iafrica.com
G U
GU
Mohangi
B
B
Rothman
A W
AW
van Zyl
N
N
Duneas
D000469:Alkaline Phosphatase / Q000032:analysis; D000818:Animals; D001857:Bone Matrix / Q000637:transplantation*; D055396:Bone Morphogenetic Protein 2 / Q000008:administration & dosage / Q000627:therapeutic use; D055415:Bone Morphogenetic Protein 4 / Q000008:administration & dosage / Q000627:therapeutic use; D055419:Bone Morphogenetic Protein 7 / Q000008:administration & dosage / Q000627:therapeutic use; D019485:Bone Morphogenetic Proteins / Q000008:administration & dosage / Q000627:therapeutic use*; D004305:Dose-Response Relationship, Drug; D005205:Fascia / Q000473:pathology; D000071938:Fasciotomy; D008297:Male; D009999:Ossification, Heterotopic / Q000473:pathology; D010012:Osteogenesis / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D040521:Subcutaneous Tissue / Q000473:pathology / Q000601:surgery; D013552:Swine; D053773:Transforming Growth Factor beta1 / Q000008:administration & dosage / Q000627:therapeutic use; D014183:Transplantation, Heterologous; D015854:Up-Regulation / Q000187:drug effects
D055396:Bone Morphogenetic Protein 2; D055415:Bone Morphogenetic Protein 4; D055419:Bone Morphogenetic Protein 7; D019485:Bone Morphogenetic Proteins; D053773:Transforming Growth Factor beta1; D000469:Alkaline Phosphatase