[Differential expressions of MDR-1, RRM-1, EGFR and ERCC-1 in primary tumors and metastatic lymph nodes of NSCLC patients].
OBJECTIVE
To investigate the differential expressions of multidrug resistance protein 1 (MDR-1), ribonucleotide reductase M1(RRM-1), epidermal growth factor receptor(EGFR), excision repair cross-complementation group 1 (ERCC-1) in primary tumors and metastatic lymph nodes of patients with non-small cell lung cancer (NSCLC), and analyze the relationship between the differential expressions and pathological types of NSCLC. Methods The expressions of MDR-1, RRM-1, EGFR and ERCC-1 were detected by immunohistochemistry in primary tumors and metastatic lymph nodes of 46 cases NSCLC patients. Wilcoxon signed ranks test was used to compare the differences in the expressions of these four molecular markers in primary lesions and metastatic lymph nodes. Fisher's exact test was used to analyze the relationship between differential expressions and pathological types of NSCLC.


RESULTS
The expressions of MDR-1, RRM-1, EGFR, ERCC-1 were observed in a variety of pathological types of NSCLC. The expression of ERCC-1 was significantly different between primary tumors and metastatic lymph nodes for lung adenocarcinoma (P=0.026). But the expressions of MDR-1, RRM-1, EGFR were not significantly different between primary tumors and metastatic lymph nodes for all pathological types of NSCLC (P>0.05). Conclusion The expression of MDR-1, RRM-1, EGFR, ERCC-1 of primary tumors and metastatic lymph nodes is found in all NSCLC. The expression of ERCC-1 is significantly different in primary tumors from in metastatic lymph nodes of lung adenocarcinoma.
Department of Cardiothoracic Surgery, General Hospital of Chinese PLA, Beijing, China. gnn304@163.com
Nannan
N
Guo
Shanshan
S
Li
Wen
W
Zhang
Changhai
C
Yu
Hongwei
H
Wang
Yiming
Y
Zhang
Jianqi
J
Yu
D018435:ATP Binding Cassette Transporter, Subfamily B; D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D000328:Adult; D000368:Aged; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D004268:DNA-Binding Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D004720:Endonucleases / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D066246:ErbB Receptors / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D005260:Female; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D012262:Ribonucleoside Diphosphate Reductase; D025521:Tumor Suppressor Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism
C513055:ABCB1 protein, human; D018435:ATP Binding Cassette Transporter, Subfamily B; D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D004268:DNA-Binding Proteins; D025521:Tumor Suppressor Proteins; C475286:RRM1 protein, human; D012262:Ribonucleoside Diphosphate Reductase; C512478:EGFR protein, human; D066246:ErbB Receptors; C071447:ERCC1 protein, human; D004720:Endonucleases