An investigation into the relationship between metabolic responses and energy regulation in antibody-producing cell.
Energy-efficient metabolic responses were often noted in high-productive cultures. To better understand these metabolic responses, an investigation into the relationship between metabolic responses and energy regulation was conducted via a comparative analysis among cultures with different energy source supplies. Both glycolysis and glutaminolysis were studied through the kinetic analyses of major extracellular metabolites concerning the fast and slow cell growth stages, respectively, as well as the time-course profiles of intracellular metabolites. In three cultures showing distinct antibody productivities, the amino acid metabolism and energy state were further examined. Both the transition of lactate from production to consumption and steady intracellular pools of pyruvate and lactate were observed to be correlated with efficient energy regulation. In addition, an efficient utilization of amino acids as the replenishment for the TCA cycle was also found in the cultures with upregulated energy metabolism. It was further revealed that the inefficient energy regulation would cause low cell productivity based on the comparative analysis of cell growth and productivity in cultures having distinct energy regulation.
The State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, P. R. China.
Ya-Ting
YT
Sun
Liang
L
Zhao
Zhao-Yang
ZY
Ye
Li
L
Fan
Xu-Ping
XP
Liu
Wen-Song
WS
Tan
D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D000906:Antibodies / Q000378:metabolism*; D016466:CHO Cells; D003412:Cricetulus; D004734:Energy Metabolism; D005973:Glutamine / Q000378:metabolism; D006019:Glycolysis; D007773:Lactates / Q000378:metabolism; D011773:Pyruvates / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism
D000596:Amino Acids; D000906:Antibodies; D007773:Lactates; D011773:Pyruvates; D011994:Recombinant Proteins; D005973:Glutamine