Association of a novel polymorphism of the β2-chimaerin gene (CHN2) with smoking.
OBJECTIVE
The CHN2 gene encodes the β2-chimaerin, a Rac-specific guanosine-5'-triphosphatase activating protein with an important role in the establishment of functional brain circuitry by controlling axon pruning. Genetic studies suggest that the CHN2 gene harbors variants that contribute to addiction vulnerability and smoking behavior. To further evaluate the role of β2-chimaerin in nicotine addiction, we investigated the association of 3 individual polymorphisms of the CHN2 gene with smoking dependence.


METHODS
Three hundred sixty-one healthy volunteers, 173 smokers (mean ± SD age, 60.4 ± 1.4 years) and 188 control subjects (mean ± SD age, 45.9 ± 1.4 years) were genotyped for 3 single-nucleotide polymorphisms in the CHN2 gene (rs3750103, rs12112301, and rs186911567). The association of these polymorphisms with smoking habits was analyzed.


RESULTS
There was no significant association of polymorphisms rs12112301 and rs3750103 with smoking. However, there was a significant difference in the frequency of the rs186911567 polymorphism between the smokers and the controls (P = 0.003).


CONCLUSIONS
We report for the first time a significant association of the novel rs186911567 polymorphism of the CHN2 gene with smoking.
From the *Unidad de Medicina Molecular, Facultad de Medicina, Hospital Universitario de Salamanca and IBMCC (CSIC/Universidad de Salamanca), Salamanca, Spain; and †Instituto de Biología y Genética Molecular (IBGM), Consejo Superior de Investigaciones Científicas (CSIC), Valladolid. Spain.
Laura
L
Barrio-Real
Miguel
M
Barrueco
Rogelio
R
González-Sarmiento
María J
MJ
Caloca
D000328:Adult; D003430:Cross-Sectional Studies; D005260:Female; D056726:Genetic Association Studies / Q000379:methods; D006801:Humans; D008297:Male; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D012907:Smoking / Q000453:epidemiology / Q000235:genetics*
D009363:Neoplasm Proteins; C079844:beta-chimaerin