Pluripotent lineage of CD133 stem cells isolated from human skin samples.
Skin stem cells are very important in cosmetics, pharmacological and regenerative medicine and burn cases. Foreskin samples surgically removed after circumcision from boys below 7 years were collected and primary epidermal cells were prepared by enzymatic and mechanical tituration method. Selecting CD133 (prominin-1) multipotent stem cell marker, enriched stem cells were analyzed by MACS using CD133 antibodies conjugated with magnetic beads. CD133 positive and negative cells with specific skin stem cells markers like - CD34 (Universal stem cells marker), CD29 (integrin beta-1) and CD49f (integrin alpha-6) immunophenotypes were screened and sorted in flowcytometer. Further the expression of four embryonic genes or transcription factors of pluripotent stem cells were analyzed for pluripotent character of sorted cells. It was found that skin stem cell markers associated with CD133 cells, differentially expressed CD34, CD29 and CD49f immunophenotyes on both positive and negative CD133 cells in FACS analysis. The embryonic stem cell markers (induced pluripotent stem cell markers) like Oct4, SOX2, Notch-2 and K19 genes were expressed in CD133 positive epidermal cells. It is therefore evident that foreskin derived epidermal stem cells showed pluripotent or multipotent nature. This finding opens up avenues for new uses of these stem cells for direct cell seeding in wound healing, surgical suturing and drug screening.
Genetics Department, Bhagwan Mahavir Medical Research Center, 10-1-1, Mahavir Marg, Masab Tank, Hyderabad 500 004, India.
Avvari Bhaskara
AB
Balaji
Kaiser
K
Jamil
Gangaraju Maruthi
GM
Ram
G Suryanarayana
GS
Raju
D000071916:AC133 Antigen; D015703:Antigens, CD / Q000378:metabolism*; D015415:Biomarkers / Q000378:metabolism; D019070:Cell Lineage / Q000235:genetics; D002469:Cell Separation; D002470:Cell Survival / Q000235:genetics; D002648:Child; D000078404:Epidermal Cells; D005434:Flow Cytometry; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006023:Glycoproteins / Q000378:metabolism*; D006801:Humans; D016130:Immunophenotyping; D008297:Male; D010455:Peptides / Q000378:metabolism*; D039904:Pluripotent Stem Cells / Q000166:cytology* / Q000378:metabolism; D011419:Propidium / Q000378:metabolism; D012867:Skin / Q000166:cytology*; D013194:Staining and Labeling
D000071916:AC133 Antigen; D015703:Antigens, CD; D015415:Biomarkers; D006023:Glycoproteins; C000606023:PROM1 protein, human; D010455:Peptides; D011419:Propidium