The differently expressed proteins in MSCs of degenerative scoliosis.
OBJECTIVE
Degenerative scoliosis (DS) is an important degenerative lumbar disease causing spinal dysfunction. The true reason or pathogenesis of DS is still unknown. Bone marrow-derived mesenchymal stem cells (BM-MSCs) are the stem/progenitor cells of the osteoblasts. The diseases associated with osteogenesis could be caused by abnormality of the MSCs. The purpose of this study was to find the differential proteins expressed in MSCs of patients with DS.


METHODS
We collected and cultured the MSCs from 12 DS patients and 12 age- and gender-matched patients with lumbar spinal stenosis. Then the MSC samples were analyzed with 2D-DIGE and MALDI-TOF-MS to find the differential proteins which were further validated by Western blot.


RESULTS
We found 115 spots that were differently expressed in the MSC of DS patients with 2D-DIGE, and 44 proteins were identified from samples of DS and control using MALDI-TOF-MS. Of these proteins, PIAS2, NDUFA2, and TRIM 68, which were up-regulated in DS more than 4 times were validated by Western blot.


CONCLUSIONS
The information obtained with this proteomics analysis will be useful in understanding the pathophysiology of DS. Further investigations on the functioning pathway, the specificity and the mechanism of these proteins will be carried out.
Department of Orthopaedics, Provincial Hospital Affiliated to Shandong University, Jinan, China.
Shijie
S
Han
Yong
Y
Zhu
Zhihong
Z
Wu
Jianguo
J
Zhang
Guixing
G
Qiu
D000328:Adult; D000368:Aged; D000954:Antigens, Surface / Q000032:analysis*; D015153:Blotting, Western; D016022:Case-Control Studies; D002454:Cell Differentiation / Q000502:physiology; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D008875:Middle Aged; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics / Q000379:methods; D012016:Reference Values; D012600:Scoliosis / Q000378:metabolism* / Q000503:physiopathology*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D000954:Antigens, Surface; D020543:Proteome