Application of a low-level laser therapy and the purified protein from natural latex (Hevea brasiliensis) in the controlled crush injury of the sciatic nerve of rats: a morphological, quantitative, and ultrastructural study.
This study analyzed the effects of a low-level laser therapy (LLLT, 15 J/cm(2), 780 nm wavelength) and the natural latex protein (P1, 0.1%) in sciatic nerve after crush injury (15 Kgf, axonotmesis) in rats. Sixty rats (male, 250 g) were allocated into the 6 groups (n = 10): CG-control group; EG-nerve exposed; IG-injured nerve without treatment; LG-crushed nerve treated with LLLT; PG-injured nerve treated with P1; and LPG-injured nerve treated with LLLT and P1. After 4 or 8 weeks, the nerve samples were processed for morphological, histological quantification and ultrastructural analysis. After 4 weeks, the myelin density and morphological characteristics improved in groups LG, PG, and LPG compared to IG. After 8 weeks, PG, and LPG were similar to CG and the capillary density was higher in the LG, PG, and LPG. In the ultrastructural analysis the PG and LPG had characteristics that were similar to the CG. The application of LLLT and/or P1 improved the recovery from the nerve crush injury, and in the long term, the P1 protein was the better treatment used, since only the application of LLLT has not reached the same results, and these treatments applied together did not potentiate the recovery.
Department of Anatomy, Institute of Biomedical Sciences, University of Sao Paulo, Sao Paulo, SP, Brazil.
Fernando José
FJ
Dias
João Paulo Mardegan
JP
Issa
Mamie Mizusaki
MM
Iyomasa
Joaquim
J
Coutinho-Netto
Ricardo Alexandre Junqueria
RA
Calzzani
Daniela Mizusaki
DM
Iyomasa
Luiz Gustavo
LG
Sousa
Sonia Regina Yokomizo
SR
de Almeida
Diego Pulzatto
DP
Cury
Ii-sei
IS
Watanabe
D000818:Animals; D028482:Hevea / Q000737:chemistry; D007840:Latex / Q000008:administration & dosage / Q000737:chemistry; D028022:Low-Level Light Therapy; D008297:Male; D009409:Nerve Crush; D010940:Plant Proteins / Q000008:administration & dosage / Q000737:chemistry / Q000302:isolation & purification; D051381:Rats; D017208:Rats, Wistar; D012584:Sciatic Nerve / Q000187:drug effects* / Q000293:injuries* / Q000528:radiation effects / Q000648:ultrastructure
D007840:Latex; D010940:Plant Proteins