BOD biosensor based on the yeast Debaryomyces hansenii immobilized in poly(vinyl alcohol) modified by N-vinylpyrrolidone.
An amperometric biosensor for assessing the biochemical oxygen demand (BOD) was formed by immobilizing Debaryomyces hansenii VKM Y-2482 yeast cells in poly(vinyl alcohol) modified by N-vinylpyrrolidone. Modification provided for a high sensitivity and stability of the bioreceptor. A high oxidative activity of the receptor element and the absence of any toxic effect of assayed compounds were shown for 34 substrates (alcohols, carbohydrates, carboxylic acids, amino acids, nitrophenols and surfactants) that may occur in wastewaters. Estimates of the measurement range and region of the linear dependence of signals on the BOD level, pH and temperature sensitivities, dependences of signals on concentrations of salts, stability, Michaelis kinetic constants and assay rates were obtained. The BOD values determined by the biosensor in assayed wastewater samples were shown to have a high correlation with those obtained by the standard dilution method.
Federal State Budgetary Educational Institution of Higher Professional Education, Tula State University, Russia.
V A
VA
Arlyapov
N Yu
NY
Yudina
L D
LD
Asulyan
S V
SV
Alferov
V A
VA
Alferov
A N
AN
Reshetilov
D057919:Biological Oxygen Demand Analysis / Q000379:methods*; D015374:Biosensing Techniques / Q000379:methods*; D018914:Cells, Immobilized / Q000378:metabolism; D055165:Debaryomyces / Q000378:metabolism*; D011142:Polyvinyl Alcohol; D011760:Pyrrolidinones; D062065:Wastewater / Q000032:analysis
D011142:Polyvinyl Alcohol; D011760:Pyrrolidinones; D062065:Wastewater; C042670:N-vinyl-2-pyrrolidinone
BOD biosensor; Immobilization in poly(vinyl alcohol) modified by N-vinylpyrrolidone; Yeast Debaryomyces hansenii