Characterization of leucocin B-KM432Bz from Leuconostoc pseudomesenteroides isolated from boza, and comparison of its efficiency to pediocin PA-1.
A bacteriocin-producing bacterium was isolated from boza and identified as Leuconostoc pseudomesenteroides KM432Bz. The antimicrobial peptide was purified and shown to be identical to other class IIa bacteriocins: leucocin A from Leuconostoc gelidum UAL-187 and Leuconostoc pseudomesenteroides QU15 and leucocin B from Leuconostoc carnosum Ta11a. The bacteriocin was named leucocin B-KM432Bz. Leucocin B-KM432Bz gene cluster encodes the bacteriocin precursor (lcnB), the immunity protein (lcnI) and the dedicated export machinery (lcnD and lcnE). A gene of unknown and non-essential function (lcnC), which is interrupted by an insertion sequence of the IS30 family, is localized between lcnB and lcnD. The activity of leucocin B-KM432Bz requires subunit C of the EII(t) Man mannose permease, which is the receptor for entry into target cells. The determination of the minimum inhibitory concentrations revealed the lowest values for leucocin B-KM432Bz over Listeria strains, with 4 to 32 fold better efficiency than pediocin PA-1.
Communication Molecules and Adaptation of Microorganisms (MCAM), UMR 7245 CNRS-MNHN, Muséum National d'Histoire Naturelle, Paris, France.
Kahina Maya
KM
Makhloufi
Alyssa
A
Carré-Mlouka
Jean
J
Peduzzi
Carine
C
Lombard
Carol Ann
CA
van Reenen
Leon Milner Theodore
LM
Dicks
Sylvie
S
Rebuffat
D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D001430:Bacteriocins / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D001628:Beverages / Q000382:microbiology*; D005520:Food Preservatives / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology; D007700:Kinetics; D007934:Leuconostoc / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D008969:Molecular Sequence Data; D005810:Multigene Family; D000070017:Pediocins; D017421:Sequence Analysis
D000900:Anti-Bacterial Agents; D001430:Bacteriocins; D005520:Food Preservatives; D000070017:Pediocins; C074288:pediocin PA-1