A computational module assembled from different protease family motifs identifies PI PLC from Bacillus cereus as a putative prolyl peptidase with a serine protease scaffold.
Proteolytic enzymes have evolved several mechanisms to cleave peptide bonds. These distinct types have been systematically categorized in the MEROPS database. While a BLAST search on these proteases identifies homologous proteins, sequence alignment methods often fail to identify relationships arising from convergent evolution, exon shuffling, and modular reuse of catalytic units. We have previously established a computational method to detect functions in proteins based on the spatial and electrostatic properties of the catalytic residues (CLASP). CLASP identified a promiscuous serine protease scaffold in alkaline phosphatases (AP) and a scaffold recognizing a β-lactam (imipenem) in a cold-active Vibrio AP. Subsequently, we defined a methodology to quantify promiscuous activities in a wide range of proteins. Here, we assemble a module which encapsulates the multifarious motifs used by protease families listed in the MEROPS database. Since APs and proteases are an integral component of outer membrane vesicles (OMV), we sought to query other OMV proteins, like phospholipase C (PLC), using this search module. Our analysis indicated that phosphoinositide-specific PLC from Bacillus cereus is a serine protease. This was validated by protease assays, mass spectrometry and by inhibition of the native phospholipase activity of PI-PLC by the well-known serine protease inhibitor AEBSF (IC50 = 0.018 mM). Edman degradation analysis linked the specificity of the protease activity to a proline in the amino terminal, suggesting that the PI-PLC is a prolyl peptidase. Thus, we propose a computational method of extending protein families based on the spatial and electrostatic congruence of active site residues.
Unidad de Biofísica, Consejo Superior de Investigaciones Científicas, Universidad del Pais Vasco/Euskal Herriko Unibertsitatea and Departamento de Bioquímica, Universidad del País Vasco, Bilbao, Spain.
Adela
A
Rendón-Ramírez
Manish
M
Shukla
Masataka
M
Oda
Sandeep
S
Chakraborty
Renu
R
Minda
Abhaya M
AM
Dandekar
Bjarni
B
Ásgeirsson
Félix M
FM
Goñi
Basuthkar J
BJ
Rao
D020816:Amino Acid Motifs; D001409:Bacillus cereus / Q000201:enzymology*; D001426:Bacterial Proteins / Q000737:chemistry*; D020134:Catalytic Domain; D003198:Computer Simulation; D008958:Models, Molecular; D054801:Phosphoinositide Phospholipase C / Q000737:chemistry*; D000085822:Prolyl Oligopeptidases; D012697:Serine Endopeptidases / Q000737:chemistry*
D001426:Bacterial Proteins; D054801:Phosphoinositide Phospholipase C; D012697:Serine Endopeptidases; D000085822:Prolyl Oligopeptidases