Rapid, isothermal DNA self-replication induced by a destabilizing lesion.
You spin me round: Using a destabilizing abasic site and high concentration of ligase, rapid DNA self-replication in an isothermal ligase chain reaction (LCR) was produced. Both destabilization and rapid ligation are essential for proper LCR replication. This method also provides insight into prebiotic nucleotide replication and is a potential amplification method for biodiagnostics.
Department of Chemistry, University of Alberta, Edmonton, AB, T6G 2G2 (Canada) http://www.chem.ualberta.ca/∼gibbsdavis.
Abu
A
Kausar
Catherine J
CJ
Mitran
Yimeng
Y
Li
Julianne M
JM
Gibbs-Davis
D002384:Catalysis; D004247:DNA / Q000096:biosynthesis / Q000138:chemical synthesis / Q000737:chemistry*; D004261:DNA Replication; D006801:Humans; D022521:Ligase Chain Reaction / Q000379:methods*; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D009841:Oligonucleotides / Q000737:chemistry*
D009841:Oligonucleotides; D004247:DNA
DNA replication; autonomous replication; isothermal DNA amplification; oligonucleotides; origins of life