[Protective effects of paeoniflorin against aBETA25-35-induced oxidative stress in PC12 cells].
OBJECTIVE
To investigate the neuroprotective effects and mechanism of paeoniflorin on Abeta25-35-induced oxidative stress in PC12 cells.


METHODS
The proliferation of induced PC12 cells were investigated by the MTT method. The leakage rate of lactate dehydrogenase (LDH), the intracellular content of malondialdehyde (MDA), superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) were also measured. The changes of the intracellular reactive oxygen species (ROS) level and the mitochondrial membrane potential (MMP) were investigated by flow cytometry using DCFH-DA and Rh123 staining. The protein expression of HO-1, Cyt C and cleaved Caspase-3 was detected by Western blot.


RESULTS
The results showed that paeoniflorin at different doses (5, 10, 20 micromol . L-1) could increase the cell viability and activities of antioxidant enzyme (GSH-Px, SOD and HO-1), prevent LDH release and lipid peroxidation (MDA) production, decrease the level of intracellular ROS, increase MMP, inhibit the release of cytochrome c from the mitochondria and attenuate activation of Caspase-3.


CONCLUSIONS
Paeoniflorin can protect PC12 cells from Abeta25-35 oxidative stress injuries. The mechanism may be related to its antioxidant action and inhibition of mitochondria-mediated caspase signaling pathways.
Department of Pharmacology, Henan University of Science and Technology, Luoyang 471003, China. liuling921@126.com
Ling
L
Liu
Shu-Ying
SY
Wang
D016229:Amyloid beta-Peptides / Q000633:toxicity*; D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D001565:Benzoates / Q000494:pharmacology*; D001952:Bridged-Ring Compounds / Q000494:pharmacology*; D053148:Caspase 3 / Q000378:metabolism; D005960:Glucosides / Q000494:pharmacology*; D005979:Glutathione Peroxidase / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008315:Malondialdehyde / Q000378:metabolism; D039821:Monoterpenes; D018384:Oxidative Stress / Q000187:drug effects; D016716:PC12 Cells; D010446:Peptide Fragments / Q000633:toxicity*; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism; D006027:Glycosides
D016229:Amyloid beta-Peptides; D000975:Antioxidants; D001565:Benzoates; D001952:Bridged-Ring Compounds; D053148:Caspase 3; D005960:Glucosides; D005979:Glutathione Peroxidase; D008315:Malondialdehyde; D039821:Monoterpenes; D010446:Peptide Fragments; D017382:Reactive Oxygen Species; D013482:Superoxide Dismutase; C074636:amyloid beta-protein (25-35); C015423:peoniflorin; D006027:Glycosides