Translational activation of developmental messenger RNAs during neonatal mouse testis development.
The basic tenets of germ cell development are conserved among metazoans. Following lineage commitment in the embryo, germ cells proliferate, transition into meiosis, and then differentiate into gametes capable of fertilization. In lower organisms such as Drosophila and C. elegans, germline stem cells make the decision to proliferate or enter meiosis based in large part on the regulated expression of genes by translational control. This study undertakes a direct characterization of mRNAs that experience translational control and their involvement in similar decisions in the mammalian testis. We previously showed that translation of mRNA encoding the germ cell-specific gene Rhox13 was suppressed in the fetal and neonatal testis. By investigating changes in message utilization during neonatal testis development, we found that a large number of mRNAs encoding both housekeeping and germ cell-specific proteins experience enhanced translational efficiency, rather than increase in abundance, in the testis as quiescent gonocytes transition to mitotic spermatogonia. Our results indicate that translational control is a significant regulator of the germ cell proteome during neonatal testis development.
Department of Anatomy and Cell Biology, Brody School of Medicine, East Carolina University, Greenville, North Carolina.
Vesna A
VA
Chappell
Jonathan T
JT
Busada
Brett D
BD
Keiper
Christopher B
CB
Geyer
D048868:Adaptor Proteins, Signal Transducing; D000818:Animals; D000831:Animals, Newborn; D002352:Carrier Proteins / Q000378:metabolism; D018797:Cell Cycle Proteins; D039642:Eukaryotic Initiation Factors; D018507:Gene Expression Regulation, Developmental; D050437:Genes, Developmental; D008297:Male; D051379:Mice; D010750:Phosphoproteins / Q000378:metabolism; D011132:Polyribosomes / Q000378:metabolism; D014176:Protein Biosynthesis / Q000235:genetics*; D012333:RNA, Messenger / Q000378:metabolism*; D038762:Ribosomal Protein S6 Kinases, 70-kDa / Q000378:metabolism; D013091:Spermatogenesis / Q000235:genetics; D013093:Spermatogonia / Q000378:metabolism / Q000502:physiology; D013737:Testis / Q000254:growth & development* / Q000378:metabolism
D048868:Adaptor Proteins, Signal Transducing; D002352:Carrier Proteins; D018797:Cell Cycle Proteins; C499527:Eif4ebp1 protein, mouse; D039642:Eukaryotic Initiation Factors; D010750:Phosphoproteins; D012333:RNA, Messenger; D038762:Ribosomal Protein S6 Kinases, 70-kDa; C448901:ribosomal protein S6 kinase, 70kD, polypeptide 1
gonocyte; spermatogenesis; spermatogonia; testis; translation