Induction of lipids and resultant FAME profiles of microalgae from coastal waters of Pearl River Delta.
This article presents a study on identification, cultivation and characterisation of microalgal strains from the coastal waters of the Pearl River Delta in Guangdong, China. Thirty-seven identified strains belong to the families: Chlorellaceae, Scotiellocystoidaceae, Scenedesmaceae,Selenastraceae,Micractiniaceae, Coccomyxaceae, Trebouxiaceae and Chlorococcaceae. Of isolated strains, Hindakia PKUAC 169 was selected for lipid induction using two methods: nitrogen starvation and salt stress. After derivatisation of algal lipids through in situ transesterification, lipid profiles of the alga under the two methods were analysed. The results have shown that both lipid yield and fatty acid profiles vary with the methods. Of the two tested methods of inducing lipid production, salt stress yielded three-fold higher lipid productivity than nitrogen starvation. The lipids are predominantly composed of C14-C18 fatty acids, which are favourable for biodiesel production. Moreover, the content of polyunsaturated fatty acids was below the limit of 12% set by EN14214 biodiesel standard.
Shenzhen Engineering Laboratory for Algal Biofuel Technology Development and Application, School of Environment and Energy, Peking University-Shenzhen Graduate School, Shenzhen 518055, China.
Maurycy
M
Daroch
Shenzhen Engineering Laboratory for Algal Biofuel Technology Development and Application, School of Environment and Energy, Peking University-Shenzhen Graduate School, Shenzhen 518055, China.
Congcong
C
Shao
Shenzhen Engineering Laboratory for Algal Biofuel Technology Development and Application, School of Environment and Energy, Peking University-Shenzhen Graduate School, Shenzhen 518055, China.
Ying
Y
Liu
Shenzhen Engineering Laboratory for Algal Biofuel Technology Development and Application, School of Environment and Energy, Peking University-Shenzhen Graduate School, Shenzhen 518055, China.
Shu
S
Geng
Shenzhen Engineering Laboratory for Algal Biofuel Technology Development and Application, School of Environment and Energy, Peking University-Shenzhen Graduate School, Shenzhen 518055, China; Department of Biological and Agricultural Engineering, North Carolina State University, Raleigh, NC 27695, USA. Electronic address: chengjy@pkusz.edu.cn.
Jay J
JJ
Cheng
D044822:Biodiversity; D056804:Biofuels; D018533:Biomass; D002681:China; D004952:Esters / Q000737:chemistry*; D005227:Fatty Acids / Q000737:chemistry; D005231:Fatty Acids, Unsaturated / Q000737:chemistry; D016013:Likelihood Functions; D008055:Lipids / Q000737:chemistry*; D058086:Microalgae / Q000235:genetics / Q000378:metabolism*; D009584:Nitrogen / Q000737:chemistry; D009821:Oils; D010802:Phylogeny; D012337:RNA, Ribosomal, 18S / Q000378:metabolism; D045483:Rivers; D012492:Salts / Q000737:chemistry
D056804:Biofuels; D004952:Esters; D005227:Fatty Acids; D005231:Fatty Acids, Unsaturated; D008055:Lipids; D009821:Oils; D012337:RNA, Ribosomal, 18S; D012492:Salts; D009584:Nitrogen
Algal biodiesel; Cultivation; Diversity; FAME; Microalgae; Oil accumulation; PKUAC; Peking University Algae Collection; RFLP; fatty acid methyl esters; restriction fragment length polymorphism