Boronic acid-functionalized core-shell-shell magnetic composite microspheres for the selective enrichment of glycoprotein.
In this work, core-shell-shell-structured boronic acid-functionalized magnetic composite microspheres Fe3O4@SiO2@poly (methyl methacrylate-co-4-vinylphenylbornoic acid) (Fe3O4@SiO2@P(MMA-co-VPBA)) with a uniform size and fine morphology were synthesized. Here, Fe3O4 magnetic particles were prepared by a solvothermal reaction, whereas the Fe3O4@SiO2 microspheres with a core-shell structure were obtained by a sol-gel process. 3-(Trimethoxysilyl) propyl methacrylate (MPS)-modified Fe3O4@SiO2 was used as the seed in the emulsion polymerization of MMA and VPBA to form the core-shell-shell-structured magnetic composite microspheres. As the boronic acid groups on the surface of Fe3O4@SiO2@P(MMA-co-VPBA) could form tight yet reversible covalent bonds with the cis-1,2-diols groups of glycoproteins, the magnetic composite microspheres were applied to enrich a standard glycoprotein, horseradish peroxidase (HRP), and the results demonstrated that the composite microspheres have a higher affinity for the glycoproteins in the presence of the nonglycoprotein bovine serum albumin (BSA) over HRP. Additionally, different monomer mole ratios of MMA/VPBA were studied, and the results implied that using MMA as the major monomer could reduce the amount of VPBA with a similar glycoprotein enrichment efficiency but a lower cost.
State Key Laboratory of Molecular Engineering of Polymers and Department of Macromolecular Science, Fudan University , 220 Handan Road, Shanghai 200433, China.
Miaorong
M
Pan
Yangfei
Y
Sun
Jin
J
Zheng
Wuli
W
Yang
D000818:Animals; D001897:Boronic Acids / Q000737:chemistry*; D002417:Cattle; D004591:Electrophoresis, Polyacrylamide Gel; D052203:Ferrosoferric Oxide / Q000737:chemistry; D006735:Horseradish Peroxidase / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D008280:Magnetics; D008863:Microspheres; D011108:Polymers / Q000737:chemistry; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry
D001897:Boronic Acids; D011108:Polymers; D012710:Serum Albumin, Bovine; D012822:Silicon Dioxide; D006735:Horseradish Peroxidase; D052203:Ferrosoferric Oxide