Methods for creating mutations in C. elegans that extend lifespan.
The principle of commonly used methods to create mutations in the nematode Caenorhabditis elegans (C. elegans) is straightforward. In general, worms are exposed to a dose of mutagen resulting in DNA damages and mutations. Screening the progeny of the mutagenized animals for a certain phenotype is the regular forward genetic approach in C. elegans. A mutant selected from such a population is stabilized to recover a pure homozygous strain. In this chapter, we categorize the protocol into mutagenesis, phenotype screen, and outcross and provide time-tested procedures for their implementation to create long-lived worm mutants.
Key Laboratory of Developmental Genes and Human Diseases in Ministry of Education, Medical School of Southeast University, Nanjing, People's Republic of China.
Dayong
D
Wang
Min
M
Cao
Jessica
J
Dinh
Yuqing
Y
Dong
D000375:Aging; D000818:Animals; D017173:Caenorhabditis elegans / Q000235:genetics* / Q000254:growth & development; D017238:Genes, Helminth / Q000235:genetics*; D008017:Life Expectancy; D008136:Longevity / Q000235:genetics*; D016296:Mutagenesis; D009153:Mutagens / Q000008:administration & dosage; D009154:Mutation / Q000235:genetics*
D009153:Mutagens