Evaluation of two commercial enzyme-linked immunosorbent assay kits for the detection of serum antibodies against Akabane virus in cattle.
In New Zealand, an arbovirus surveillance program has been operating for more than 20 years, which includes testing of cattle with the Akabane virus neutralization test. With the aim to replace this laborious test by an easier-to-perform enzyme-linked immunosorbent assay (ELISA), 2 commercial ELISA kits, ELISA-1 from France (originally from Australia) and ELISA-2 from Japan, were compared, using 334 serum samples from noninfected New Zealand cattle, and 548 serum samples from naturally infected cattle herds in Australia. Diagnostic specificities for the test methods were high, ranging from 99.4% to 100%. The diagnostic sensitivities varied considerably between the test methods and differed from the values reported by the manufacturers (94% for each ELISA). The diagnostic sensitivities relative to the virus neutralization test (n = 378) were 96.0% for ELISA-1 or 98.9% when suspect samples were included, and 78.0% for ELISA-2. Differences in the commercial ELISA kits may be explained by the presence of other Simbu serogroup viruses in Australian cattle herds, causing cross-reactions in ELISA-1. Both commercial ELISA kits would be fit for purpose and could replace the virus neutralization test for Akabane virus surveillance in New Zealand. ELISA-1 may be able to detect other Simbu serogroup viruses, should they be present. The current study shows that despite comparable ELISA test characteristics given by the manufacturers, evaluation on the target population revealed marked differences in the ELISA kits test methods' characteristics.
1Reinhold Kittelberger, Investigation and Diagnostic Centres and Response, Wallaceville, Ministry for Primary Industries, PO Box 40742, Upper Hutt 5140, New Zealand.reinhold.kittelberger@mpi.govt.nz.
Reinhold
R
Kittelberger
Andrew M J
AM
McFadden
Peter D
PD
Kirkland
Michaela J
MJ
Hannah
Della
D
Orr
Rudolfo
R
Bueno
Richard
R
Swainsbury
Denise
D
Keen
Judy
J
Jenner
Jennifer
J
French
Clive J
CJ
Pigott
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D002044:Bunyaviridae Infections / Q000097:blood / Q000662:veterinary* / Q000821:virology; D002417:Cattle; D002418:Cattle Diseases / Q000097:blood / Q000821:virology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000592:standards / Q000662:veterinary*; D009520:New Zealand; D016851:Orthobunyavirus / Q000302:isolation & purification*; D012372:ROC Curve; D012680:Sensitivity and Specificity
D000914:Antibodies, Viral
Akabane virus; Simbu virus; cross-reactivity; enzyme-linked immunosorbent assay; evaluation; sensitivity; specificity; virus neutralization test