Enhanced gellan gum production by hydrogen peroxide (H2O 2) induced oxidative stresses in Sphingomonas paucimobilis.
In this study, the effect of H2O2-induced oxidative stress on gellan gum production and cell growth were investigated. Gellan gum production was improved and cell growth was inhibited by H2O2. A multiple H2O2 stresses with different concentrations were developed to optimize gellan gum production. A maximal gellan gum yield (22.52 g/L), which was 35.58 % higher than the control, was observed with 2, 2, 3, 4 mmol/L H2O2 added at 6, 12, 18, 24 h, respectively. Moreover, UDP-glucose pyrophosphorylase activity and glucosyltransferase activity were increased with H2O2 stresses. This new strategy of multiple H2O2-induced oxidative stresses would be further applied to gellan gum production in future study.
Department of Life Science, Hefei Normal University, Hefei, AnHui, 230061, China, zhu_guilan@yahoo.com.cn.
Guilan
G
Zhu
Long
L
Sheng
Qunyi
Q
Tong
D006861:Hydrogen Peroxide / Q000494:pharmacology*; D016877:Oxidants / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects*; D011135:Polysaccharides, Bacterial / Q000378:metabolism*; D020578:Sphingomonas / Q000378:metabolism*
D016877:Oxidants; D011135:Polysaccharides, Bacterial; C048288:gellan gum; D006861:Hydrogen Peroxide