Selective sensing of Hg²⁺ using rhodamine-thiophene conjugate: red light emission and visual detection of intracellular Hg²⁺ at nanomolar level.
Rhodamine-thiophene conjugate (L) has been synthesized and characterized by (1)H NMR, FTIR and mass spectra. L shows a large enhancement in emission intensity in presence of Hg(2+). Moreover, naked eye color of L becomes intense red in presence of Hg(2+). The lowest detection limit for Hg(2+) is 1 × 10(-9)M in HEPES buffer (0.1M in EtOH/water, 1/1, v/v, pH 7.4). Hg(2+) induced chelation enhanced fluorescence (CHEF) is associated with spirolacram ring opening of the rhodamine unit. Trace level intracellular Hg(2+) is visualized under fluorescence microscope.
Department of Chemistry, The University of Burdwan, Burdwan 713104, India.
Sandip
S
Mandal
Arnab
A
Banerjee
Sisir
S
Lohar
Amarnath
A
Chattopadhyay
Bidisha
B
Sarkar
Subhra Kanti
SK
Mukhopadhyay
Animesh
A
Sahana
Debasis
D
Das
D029062:Apocynaceae / Q000378:metabolism; D002176:Candida albicans / Q000378:metabolism; D005456:Fluorescent Dyes / Q000737:chemistry*; D008027:Light; D008628:Mercury / Q000032:analysis* / Q000378:metabolism; D011058:Pollen / Q000378:metabolism; D012235:Rhodamines / Q000737:chemistry*; D013876:Thiophenes / Q000737:chemistry*
D005456:Fluorescent Dyes; D012235:Rhodamines; D013876:Thiophenes; D008628:Mercury
Cell imaging; Fluorescence; Mercury; Naked eye detection; Real sample analysis; Rhodamine–thiophene