Nitric oxide mediates coral bleaching through an apoptotic-like cell death pathway: evidence from a model sea anemone-dinoflagellate symbiosis.
Coral bleaching (involving the loss of symbiotic algae from the cnidarian host) is a major threat to coral reefs and appears to be mediated at the cellular level by nitric oxide (NO). In this study, we examined the specific role of NO in bleaching using the sea anemone Aiptasia pulchella, a model system for the study of corals. Exposure of A. pulchella to high-temperature shock (26-33°C over <1 h) or an NO donor (S-nitrosoglutathione) resulted in significant increases in host caspase-like enzyme activity. These responses were reflected in the intensities of bleaching, which were significantly higher in heat- or NO-treated specimens than in controls maintained in seawater at 26°C. Notably, the inhibition of caspase-like activity prevented bleaching even in the presence of an NO donor or at elevated temperature. The additional use of an NO scavenger controlled for effects mediated by agents other than NO. We also exposed A. pulchella to a more ecologically relevant treatment (an increase from 26 to 33°C over 6-7 d). Again, host NO synthesis correlated with the activation of caspase-like enzyme activity. Therefore, we conclude that NO's involvement in cnidarian bleaching arises through the regulation of host apoptotic pathways.
1School of Biological Sciences, Victoria University of Wellington, Kelburn Parade, Wellington, 6140, New Zealand. Email: simon.davy@vuw.ac.nz.
Thomas D
TD
Hawkins
Benjamin J
BJ
Bradley
Simon K
SK
Davy
D000818:Animals; D017209:Apoptosis; D020169:Caspases / Q000378:metabolism; D004141:Dinoflagellida / Q000378:metabolism* / Q000502:physiology; D006358:Hot Temperature; D009569:Nitric Oxide / Q000096:biosynthesis* / Q000378:metabolism; D026422:S-Nitrosoglutathione / Q000494:pharmacology; D012615:Sea Anemones / Q000378:metabolism* / Q000502:physiology; D013559:Symbiosis
D009569:Nitric Oxide; D026422:S-Nitrosoglutathione; D020169:Caspases
Symbiodinium; heat stress; innate immunity; zooxanthellae