Specific detection of inflamed cells using TLR1 antibody and its secondary antibody-conjugated nano-beads.
Chronic inflammation can lead to several diseases, thus analysis of the inflammation state of live cells may have important clinical applications. However, there is not currently well-established method for specific detection of inflamed cells via live cell imaging. In this study, we developed an effective antibody (Ab)-based cell imaging method for the detection of inflamed cells using Ab-conjugated nano-beads. Several receptors were tested as potential biomarkers for cell inflammation, and corresponding fluorescence-labeled Abs and/or Ab-conjugated nano-beads were used to detect inflamed cells via fluorescence imaging. Interestingly, when we employed sequential use of TLR1 primary Ab and size-optimized nano-beads conjugated with secondary Ab, we were able to clearly discriminate inflamed cells from normal ones. The Ab-based cell-imaging method described herein provides an important basis for the development of high-throughput analysis of cell inflammation, potentially leading to the identification of factors involved in inflammation and anti-inflammatory drug candidates.
Department of Biotechnology and Bioinformatics, Korea University, Jochiwon, Sejong, Republic of Korea.
Ki Baek
KB
Yeo
Unhwan
U
Ha
Yong Woo
YW
Jung
Taek Jin
TJ
Kang
Seung Pil
SP
Pack
D000918:Antibody Specificity; D015415:Biomarkers / Q000378:metabolism; D002460:Cell Line; D005456:Fluorescent Dyes; D006801:Humans; D007249:Inflammation / Q000175:diagnosis* / Q000276:immunology*; D058726:Nanoconjugates; D051194:Toll-Like Receptor 1 / Q000276:immunology / Q000378:metabolism*
D015415:Biomarkers; D005456:Fluorescent Dyes; D058726:Nanoconjugates; D051194:Toll-Like Receptor 1
1°Ab(TLR1); 2°Ab(fluorescent dye); DMSO; DSG; FBS; Inflammation; Nano-sized beads; PBS; TLR1; TLR1 antibody; TLR1 primary antibody; Toll-like receptor 1; dimethyl sulfoxide; disuccinimidyl glutarate; fetal bovine serum; phosphate-buffered saline; secondary antibody labeled with fluorescent dye