A mutation in the gene for polynucleotide kinase of bacteriophage T4 K10 affects mRNA processing.
The bacteriophage T4 insertion-substitution (I/S) vector system has become one of the most important tools for the introduction of site-directed mutations into the T4 genome. In this study, we show that the I/S phage T4 K10 carries two point mutations within the gene for polynucleotide kinase pseT, resulting in amino acid substitutions G14D and R229H. The G14D mutation impairs 5'-kinase activity in vivo as well as in vitro and leads to diminished processing at secondary sites of several RegB-cleaved transcripts.
Department of Molecular Microbiology and Biotechnology, Institute of Biochemistry of Vilnius University, Mokslininkų 12, 08662, Vilnius, Lithuania.
Živilė
Ž
Strazdaitė-Žielienė
Aurelija
A
Zajančkauskaitė
Laura
L
Kalinienė
Rolandas
R
Meškys
Lidija
L
Truncaitė
D019943:Amino Acid Substitution; D017122:Bacteriophage T4 / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism*; D050505:Mutant Proteins / Q000235:genetics / Q000378:metabolism; D020125:Mutation, Missense; D011116:Polynucleotide 5'-Hydroxyl-Kinase / Q000235:genetics* / Q000378:metabolism*; D012323:RNA Processing, Post-Transcriptional; D012333:RNA, Messenger / Q000378:metabolism*
D050505:Mutant Proteins; D012333:RNA, Messenger; D011116:Polynucleotide 5'-Hydroxyl-Kinase