Hemoglobin as a major binding protein for methylmercury in white-sided dolphin liver.
As methylmercury (MeHg) can be bioaccumulated and biomagnified in the trophic web, its toxicity for marine mammals is of major concern. Mercury speciation in marine biota has been widely studied, mainly focused on the discrimination and quantification of inorganic Hg and MeHg. Less attention has been paid to the interactions of Hg with biomolecules and the characterization of its specific binding, which play a key role in metabolic pathways controlling its uptake, transformation, and toxicity. In the studied white-sided dolphin (Lagenorhynchus acutus) liver homogenate (QC04LH4) sample, approximately 60% of the total MeHg was found in the water soluble fraction, specifically associated with high molecular weight biomolecules. The identity of the involved proteins was investigated (after tryptic digestion of the fraction) by μRPLC with parallel detection by ICP-MS and ESI-MS/MS. Molecular mass spectrometry experiments were carried out at high resolution (100000) to ensure accurate protein identification and determination of the MeHg binding sites. Cysteine residue on the dolphin hemoglobin β chain was found to be the main MeHg binding site, suggesting that hemoglobin is a major MeHg binding protein in this marine mammal and could be a potential carrier of this MeHg from blood to liver prior to its degradation in this organ. In parallel, a significant proportion of selenium was found to be present as selenoneine and a potential role for this compound in Hg detoxification is discussed.
Laboratoire de Chimie Analytique Bio-Inorganique et Environnement, CNRS UMR 5254 IPREM, Université de Pau et des Pays de l'Adour, 2 Avenue Pierre Angot, 64053, Pau, France.
Zoyne Pedrero
ZP
Zayas
Laurent
L
Ouerdane
Sandra
S
Mounicou
Ryszard
R
Lobinski
Mathilde
M
Monperrus
David
D
Amouroux
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004289:Dolphins / Q000378:metabolism*; D006454:Hemoglobins / Q000378:metabolism*; D008099:Liver / Q000737:chemistry / Q000378:metabolism*; D008767:Methylmercury Compounds / Q000032:analysis / Q000378:metabolism*; D011485:Protein Binding; D014874:Water Pollutants, Chemical / Q000032:analysis / Q000378:metabolism
D006454:Hemoglobins; D008767:Methylmercury Compounds; D014874:Water Pollutants, Chemical