Expression of melatonin synthesizing enzymes in Helicobacter pylori infected gastric mucosa.
Helicobacter pylori colonization of gastric mucosa causes pain of unknown etiology in about 15-20% of infected subjects. The aim of the present work was to determine the level of expression of enzymes involved in the synthesis of melatonin in gastric mucosa of asymptomatic and symptomatic H. pylori infected patients. To diagnose H. pylori infection, histological analysis and the urea breath test (UBT C13) were performed. The levels of mRNA expression of arylalkylamine-N-acetyltransferase (AA-NAT) and acetylserotonin methyltransferase (ASMT) were estimated in gastric mucosa with RT-PCR. The level of AA-NAT expression and AMST was decreased in H. pylori infected patients and was increased after H. pylori eradication. We conclude that decreased expression of melatonin synthesizing enzymes, AA-NAT and ASMT, in patients with symptomatic H. pylori infection returns to normal level after H. pylori eradication.
Department of Gastroenterology, Medical University of Lodz, Lodz, Poland. gastrologia@umed.lodz.pl
Cezary
C
Chojnacki
Tomasz
T
Popławski
Janusz
J
Blasiak
Jan
J
Chojnacki
Russel J
RJ
Reiter
Grazyna
G
Klupinska
D000121:Acetylserotonin O-Methyltransferase / Q000096:biosynthesis*; D000328:Adult; D047068:Arylalkylamine N-Acetyltransferase / Q000096:biosynthesis*; D001944:Breath Tests; D005260:Female; D005753:Gastric Mucosa / Q000378:metabolism / Q000382:microbiology / Q000473:pathology; D015964:Gene Expression Regulation, Bacterial; D016481:Helicobacter Infections / Q000175:diagnosis* / Q000382:microbiology / Q000473:pathology; D016480:Helicobacter pylori / Q000201:enzymology / Q000472:pathogenicity*; D006801:Humans; D008297:Male; D008550:Melatonin / Q000096:biosynthesis*; D008875:Middle Aged; D012333:RNA, Messenger / Q000096:biosynthesis; D014508:Urea / Q000378:metabolism
D012333:RNA, Messenger; D014508:Urea; D000121:Acetylserotonin O-Methyltransferase; D047068:Arylalkylamine N-Acetyltransferase; D008550:Melatonin