Effect of exogenous sucrose on the enzymes of starch degradation and sucrose metabolism in cowpea (Vigna unguiculata L.) seedlings.
Addition of exogenous sucrose and mannitol in the growth medium decreased the germination and growth of cowpea (Vigna unguiculata L. cv C-88) seedlings. The reduced seedling growth appeared to be due to the decreased acid invertase activity in growing parts of the seedlings. An exogenous supply of sucrose upregulated the sucrose phosphate synthase (SPS) activity in different parts of seedlings. Decreased amylase activity in cotyledons and the mobilization of starch from cotyledons to the growing axis was observed in the presence of exogenous sucrose and mannitol. High sucrose content observed in different tissues in the presence of exogenous sucrose and mannitol was possibly due to high SPS and low acid invertase activities and reduced conversion of sucrose to starch. It appears that exogenous sucrose acts mainly as an osmoticum, rather than a source of carbon for the growing seedlings.
Department of Biochemistry and Chemistry, Punjab Agricultural University, Ludhiana 141 004, India. nkaur@rediffmail.com
Narinder
N
Kaur
Harpreet
H
Kaur
Anil K
AK
Gupta
D000681:Amylases / Q000378:metabolism*; D018548:Cotyledon / Q000187:drug effects / Q000201:enzymology* / Q000254:growth & development; D007887:Fabaceae / Q000187:drug effects / Q000201:enzymology* / Q000254:growth & development; D018525:Germination; D005964:Glucosyltransferases / Q000378:metabolism*; D008353:Mannitol / Q000494:pharmacology; D036226:Seedlings / Q000187:drug effects / Q000201:enzymology* / Q000254:growth & development; D013213:Starch / Q000378:metabolism*; D013395:Sucrose / Q000494:pharmacology*; D013549:Sweetening Agents / Q000494:pharmacology
D013549:Sweetening Agents; D008353:Mannitol; D013395:Sucrose; D013213:Starch; D005964:Glucosyltransferases; C022698:sucrose-phosphate synthase; D000681:Amylases