RHD PCR of D-Negative Blood Donors.
RHD PCR of blood donors may be used to reveal weak D, partial D, DEL and chimeric D+/D- donors among presumed D-negative blood donors. Units donated by such donors pose a definite yet low risk for anti-D immunization of transfusion recipients. The frequency of DEL donors among D-negative donors is 1:350 to 1:2,000 in Europe and up to 1:5 in Asian countries. Different strategies for RHD PCR of blood donors have been used. Probably, the most cost-efficient implementation is replacement of sensitive D antigen testing with the indirect antiglobulin test by RHD PCR in pools which might even reduce total testing cost.
DRK Blutspendedienst NSTOB, Springe, Germany.
Franz F
FF
Wagner
Antigen D; Blood donors; DEL; Indirect antiglobulin test; RHD PCR; Rh blood group; Weak D