Nitrogen deposition weakens plant-microbe interactions in grassland ecosystems.
Soil carbon (C) and nitrogen (N) stoichiometry is a main driver of ecosystem functioning. Global N enrichment has greatly changed soil C : N ratios, but how altered resource stoichiometry influences the complexity of direct and indirect interactions among plants, soils, and microbial communities has rarely been explored. Here, we investigated the responses of the plant-soil-microbe system to multi-level N additions and the role of dissolved organic carbon (DOC) and inorganic N stoichiometry in regulating microbial biomass in semiarid grassland in northern China. We documented a significant positive correlation between DOC and inorganic N across the N addition gradient, which contradicts the negative nonlinear correlation between nitrate accrual and DOC availability commonly observed in natural ecosystems. Using hierarchical structural equation modeling, we found that soil acidification resulting from N addition, rather than changes in the plant community, was most closely related to shifts in soil microbial community composition and decline of microbial respiration. These findings indicate a down-regulating effect of high N availability on plant-microbe interactions. That is, with the limiting factor for microbial biomass shifting from resource stoichiometry to soil acidity, N enrichment weakens the bottom-up control of soil microorganisms by plant-derived C sources. These results highlight the importance of integratively studying the plant-soil-microbe system in improving our understanding of ecosystem functioning under conditions of global N enrichment.
State Key Laboratory of Vegetation and Environmental Change, Institute of Botany, Chinese Academy of Sciences, Beijing, 100093, China; University of Chinese Academy of Sciences, Beijing, 100049, China.
Cunzheng
C
Wei
Qiang
Q
Yu
Edith
E
Bai
Xiaotao
X
Lü
Qi
Q
Li
Jianyang
J
Xia
Paul
P
Kardol
Wenju
W
Liang
Zhengwen
Z
Wang
Xingguo
X
Han
D002244:Carbon / Q000378:metabolism*; D002681:China; D004777:Environment; D009584:Nitrogen / Q000378:metabolism*; D018521:Plant Physiological Phenomena; D012987:Soil / Q000737:chemistry*; D012988:Soil Microbiology
D012987:Soil; D002244:Carbon; D009584:Nitrogen
N saturation; aboveground-belowground linkages; compensatory effects; microbial carbon limitation; resource stoichiometry; structural equation modeling