Adaptation of Dekkera bruxellensis to lignocellulose-based substrate.
Adaptation of Dekkera bruxellensis to lignocellulose hydrolysate was investigated. Cells of D. bruxellensis were grown for 72 and 192 H in batch and continuous culture, respectively (adapted cells). Cultivations in semisynthetic medium were run as controls (nonadapted cells). To test the adaptation, cells from these cultures were reinoculated in the lignocellulose medium, and growth and ethanol production characteristics were monitored. Cells adapted to lignocellulose hydrolysate had a shorter lag phase, grew faster, and produced a higher ethanol concentration as compared with nonadapted cells. A stability test showed that after cultivation in rich medium, cells partially lost the adapted phenotype but still showed faster growth and higher ethanol production as compared with nonadapted cells. Because alcohol dehydrogenase genes have been described to be involved in the adaptation to furfural in Saccharomyces cerevisiae, an analogous mechanism of adaptation to lignocelluloses hydrolysate of D. bruxellensis was hypothesized. However, gene expression analysis showed that genes homologous to S. cerevisiae ADH1 were not involved in the adaptation to lignocelluloses hydrolysate in D. bruxellensis.
Uppsala Biocenter, Department of Microbiology, Swedish University of Agricultural Sciences, Box 7025750 07, Uppsala, Sweden.
Ievgeniia A
IA
Tiukova
Will
W
de Barros Pita
David
D
Sundell
Majid
M
Haddad Momeni
Svein Jarle
SJ
Horn
Jerry
J
Ståhlberg
Marcos Antonio
MA
de Morais
Volkmar
V
Passoth
D000222:Adaptation, Physiological; D000426:Alcohol Dehydrogenase / Q000235:genetics; D061249:Batch Cell Culture Techniques; D001709:Biotechnology; D055167:Dekkera / Q000166:cytology* / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D000431:Ethanol / Q000378:metabolism; D005285:Fermentation; D006868:Hydrolysis; D008031:Lignin / Q000378:metabolism*; D010641:Phenotype; D014158:Transcription, Genetic
C036909:lignocellulose; D000431:Ethanol; D008031:Lignin; D000426:Alcohol Dehydrogenase
Dekkera bruxellensis; adaptation; fermentation; lignocellulose