Whole cell entrapment techniques.
Microbial whole cells are efficient, ecological, and low-cost catalysts that have been successfully applied in the pharmaceutical, environmental, and alimentary industries, among others. Microorganism immobilization is a good way to carry out the bioprocess under preparative conditions. The main advantages of this methodology lie in their high operational stability, easy upstream separation and bioprocess scale-up feasibility. Cell entrapment is the most widely used technique for whole cell immobilization. This technique-in which the cells are included within a rigid network-is porous enough to allow the diffusion of substrates and products, protects the selected microorganism from the reaction medium, and has high immobilization efficiency (100 % in most cases).
Laboratorio de Investigaciones en Biotecnología Sustentable, Universidad Nacional de Quilmes, Bernal, Argentina.
Jorge A
JA
Trelles
Cintia W
CW
Rivero
D000180:Acrylic Resins / Q000737:chemistry; D000362:Agar / Q000737:chemistry; D000464:Alginates / Q000737:chemistry; D001419:Bacteria / Q000166:cytology; D055162:Biocatalysis; D019149:Bioreactors; D018914:Cells, Immobilized / Q000737:chemistry; D048271:Chitosan / Q000737:chemistry; D005782:Gels / Q000737:chemistry; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D010938:Plant Oils / Q000737:chemistry; D058105:Polymerization; D012685:Sepharose / Q000737:chemistry; D000074242:Sunflower Oil
D000180:Acrylic Resins; D000464:Alginates; D005782:Gels; D006603:Hexuronic Acids; D010938:Plant Oils; D000074242:Sunflower Oil; C016680:polyacrylamide gels; D020723:Glucuronic Acid; D000362:Agar; D012685:Sepharose; D048271:Chitosan