L-Arginine supplementation improves antioxidant defenses through L-arginine/nitric oxide pathways in exercised rats.
l-Arginine (l-Arg) supplementation has been shown to enhance physical exercise capacity and delay onset of fatigue. This work investigated the potential beneficial mechanism(s) of l-Arg supplementation by examining its effect on the cellular oxidative and nitrosative stress pathways in the exercised rats. Forty-eight rats were randomly divided into six groups: sedentary control; sedentary control with l-Arg treatment; endurance training (daily swimming training for 8 wk) control; endurance training with l-Arg treatment; an exhaustive exercise (one time swimming to fatigue) control; and an exhaustive exercise with l-Arg treatment. l-Arg (500 mg/kg body wt) or saline was given to rats by intragastric administration 1 h before the endurance training and the exhaustive swimming test. Expression levels and activities of the l-Arg/nitric oxide (NO) pathway components and parameters of the oxidative stress and antioxidant defense capacity were investigated in l-Arg-treated and control rats. The result show that the l-Arg supplementation completely reversed the exercise-induced activation of NO synthase and superoxide dismutase, increased l-Arg transport capacity, and increased NO and anti-superoxide anion levels. These data demonstrate that l-Arg supplementation effectively reduces the exercise-induced imbalance between oxidative stress and antioxidant defense capacity, and this modulation is likely mediated through the l-Arg/NO pathways. The findings of this study improved our understanding of how l-Arg supplementation prevents elevations of reactive oxygen species and favorably enhances the antioxidant defense capacity during physical exercise.
Department of Biochemistry Pharmacy, School of Chemistry and Life Science, SuZhou University, SuZhou, China.
Lingling
L
Shan
Bin
B
Wang
Guizhen
G
Gao
Wengen
W
Cao
Yunkun
Y
Zhang
D026923:Amino Acid Transport Systems, Basic / Q000378:metabolism; D000818:Animals; D000975:Antioxidants / Q000378:metabolism / Q000494:pharmacology*; D001012:Aorta, Abdominal / Q000187:drug effects* / Q000378:metabolism; D001120:Arginine / Q000378:metabolism / Q000494:pharmacology*; D019587:Dietary Supplements; D019344:Lactic Acid / Q000097:blood; D008297:Male; D018763:Muscle Fatigue / Q000187:drug effects; D018482:Muscle, Skeletal / Q000187:drug effects* / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism*; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D010805:Physical Conditioning, Animal; D005082:Physical Exertion; D051381:Rats; D017207:Rats, Sprague-Dawley; D057185:Sedentary Behavior; D015398:Signal Transduction / Q000187:drug effects*; D013482:Superoxide Dismutase / Q000378:metabolism; D013550:Swimming; D013997:Time Factors; D014443:Tyrosine / Q000031:analogs & derivatives / Q000378:metabolism
D026923:Amino Acid Transport Systems, Basic; D000975:Antioxidants; C490274:Slc7a2 protein, rat; D009569:Nitric Oxide; D019344:Lactic Acid; C002744:3-nitrotyrosine; D014443:Tyrosine; D001120:Arginine; D052247:Nitric Oxide Synthase Type II; C496318:Nos2 protein, rat; D013482:Superoxide Dismutase
an exhaustive exercise; endurance training; l-arginine; supplementation effect