[Relation between expression of cerebral beta-APP in the chronic alcoholism rats and death caused by TSAH].
OBJECTIVE
By observing the cerebral beta-amyloid precursor protein (beta-APP) expression in the chronic alcoholism rats with slight cerebral injury, to discuss the correlation of chronic alcoholism and death caused by traumatic subarachnoid haemorrhage (TSAH).


METHODS
Sixty male SD rats were randomly divided into watering group, watering group with strike, alcoholism group and alcoholism group with strike. Among them, the alcohol was used for continuous 4 weeks in alcoholism groups and the concussion was made in groups with strike. In each group, HE staining and immunohistochemical staining of the cerebral tissues were done and the results were analyzed by the histopathologic image system.


RESULTS
In watering group, there was no abnormal. In watering group with strike, mild neuronic congestion was found. In alcoholism group, vascular texture on cerebral surface was found. And the neurons arranged in disorder with dilated intercellular space. In alcoholism group with strike, diffuse congestion on cerebral surface was found. And there was TSAH with thick-layer patches around brainstem following irregular axonotmesis. The quantity of beta-APP IOD in alcoholism group was significantly higher in the frontal lobe, hippocampus, cerebellum, brainstem than those in watering group with strike and alcoholism group with strike.


CONCLUSIONS
The cerebral tissues with chronic alcoholism, due to the decreasing tolerance, could cause fatal TSAH and pathological changes in cerebral tissues of rats under slight cerebral injury.
Department of Forensic Medicine, Hubei University of Medicine, Shiyan 442000, China. weilai1226@126.com
Lai
L
Wei
Huai-Cheng
HC
Lei
Xiao-Jun
XJ
Yu
Xiao-Ping
XP
Lai
Hong
H
Qian
Xiao-Hu
XH
Xu
Fang-Cheng
FC
Zhu
D000437:Alcoholism / Q000150:complications* / Q000378:metabolism / Q000473:pathology; D016564:Amyloid beta-Protein Precursor / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000378:metabolism* / Q000473:pathology; D001924:Brain Concussion / Q000150:complications* / Q000378:metabolism / Q000473:pathology; D004195:Disease Models, Animal; D000431:Ethanol / Q000009:adverse effects; D008297:Male; D009474:Neurons / Q000378:metabolism / Q000473:pathology; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D020206:Subarachnoid Hemorrhage, Traumatic / Q000209:etiology* / Q000401:mortality / Q000473:pathology
D016564:Amyloid beta-Protein Precursor; D000431:Ethanol