Mitochondria-targeted antiaging gene therapy with adeno-associated viral vectors.
Transgenic expression of catalase in mitochondria using a transgenic strategy extends life span and prevents aging-related pathology in mice. However, transgenic overexpression is not suitable for a clinical application. Adeno-associated virus (AAV) is the most promising gene delivery vehicle. Here we outline strategies on the generation of an AAV vector expressing the mitochondria-targeted catalase gene (AV.RSV.MCAT). We also describe methods for evaluating physiological impact of AV.RSV.MCAT on muscle contractility and running performance in mice.
Department of Occupational Environmental Health, School of Public Health, Wuhan University, Wuhan, People's Republic of China.
Dejia
D
Li
Dongsheng
D
Duan
D000375:Aging / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D002374:Catalase / Q000235:genetics* / Q000378:metabolism; D002460:Cell Line; D000229:Dependovirus / Q000235:genetics; D018014:Gene Transfer Techniques; D015316:Genetic Therapy / Q000379:methods*; D005822:Genetic Vectors; D057809:HEK293 Cells; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL; D008928:Mitochondria / Q000235:genetics*; D009043:Motor Activity / Q000235:genetics; D009119:Muscle Contraction / Q000235:genetics; D017382:Reactive Oxygen Species / Q000378:metabolism
D017382:Reactive Oxygen Species; D002374:Catalase