[Growth and accumulation of shikonin compounds of two kinds of cells in suspension culture of Arnebia euchroma].
Via studying the phenotype, growth curve and secondary metabolites of two kinds of suspension culture cell of Arnebia euchroma, the kinetics parameters of growth and accumulation of shikonin compounds in cell suspension culture of A. euchroma was obtained through simulating and modeling. This Study found that the red high-yielding one was a fine cell line for producing shikonin compounds, and the white low-yielding one may be a mutant. The first-order and second-order derivative of the fitting function were obtained by fitting the Logistic model of growth curve to get the growth rate and growth acceleration curve of the suspended cells. It is found that the best period to subculture was the 15th day cultured in fresh medium, and the best period of the induction process was the 13th-14th day. When compared the growth rate of the red line and the shikonin compounds accumulation curve, it is found that the rapid growth of the biomass of cells was not conducive to the synthesis and accumulation of shikonin compounds.
National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China. mmcniu@163.com
Sheng
S
Wang
Teng
T
Xie
He-Chun
HC
Ye
Shu-Fang
SF
Lin
Yan
Y
Zhang
Mei-Lan
ML
Chen
Lu-Qi
LQ
Huang
Lan-Ping
LP
Guo
D028082:Boraginaceae / Q000737:chemistry / Q000166:cytology* / Q000378:metabolism*; D018929:Cell Culture Techniques; D049109:Cell Proliferation; D009285:Naphthoquinones / Q000378:metabolism*; D059828:Plant Cells
D009285:Naphthoquinones; C016101:shikonin