The molecular characterisation of a Sida-infecting begomovirus from Jamaica.
The complete DNA sequence of both genome components of a new begomovirus (Sida golden mosaic Buckup virus-[Jamaica:St. Elizabeth:2004]; SiGMBuV-[JM:SE:04]) was determined from a field-infected Sida sp. sample from Buckup, St. Elizabeth, Jamaica. Phylogenetically, both genome components of SiGMBuV-[JM:SE:04] are most closely related to malvaceous weed-infecting Floridian and Mexican begomoviruses. Its DNA-B is a recombinant molecule, the majority of which was derived from a virus resembling Sida yellow mosaic Yucatan virus-[Mexico:Yucatan:2005] (SiYMYuV-[MX:Yuc:05]), while nucleotides 43-342 were derived from a virus resembling Sida golden mosaic virus-[United States of America:Florida] (SiGMV-[US:Flo]). Symptomatic infectivity of our cloned SiGMBuV-[JM:SE:04] components was confirmed in Nicotiana benthamiana.
Biotechnology Centre, 2 St. John's Close, University of the West Indies, Mona, Kingston 7, Jamaica, cherylsstewart@gmail.com.
Cheryl
C
Stewart
Tatsuya
T
Kon
Maria
M
Rojas
André
A
Graham
Darren
D
Martin
Robert
R
Gilbertson
Marcia
M
Roye
D053839:Begomovirus / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity; D016000:Cluster Analysis; D004279:DNA, Viral / Q000737:chemistry* / Q000235:genetics*; D016679:Genome, Viral; D007563:Jamaica; D019660:Malvaceae / Q000821:virology; D008969:Molecular Sequence Data; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology; D011995:Recombination, Genetic; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D014026:Nicotiana / Q000821:virology
D004279:DNA, Viral