Role of nuclear localization of PSMB1 in transcriptional activation.
The production of retinol binding protein 4 (RBP4) is higher in adipose tissue in type 2 diabetes. We have found that proteasome subunit beta type 1 (PSMB1) is a transcriptional activator of Rbp4. In the present study, the putative tyrosine phosphorylation site in PSMB1 was mutated to phenylalanine. The mutated form of PSMB1 displayed increased nuclear translocation, resulting in activation of transcription in adipocytes.
Section of Food and Nutrition Labeling, Department of Food Function and Labeling, National Institute of Health and Nutrition.
Jun
J
Yamauchi
Mariko
M
Sekiguchi
Tomomi
T
Shirai
Mami
M
Yamada
Yoshiko
Y
Ishimi
D017667:Adipocytes / Q000378:metabolism; D003924:Diabetes Mellitus, Type 2 / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D053652:Nuclear Transfer Techniques; D046988:Proteasome Endopeptidase Complex / Q000235:genetics / Q000378:metabolism*; D054839:Retinol-Binding Proteins, Plasma / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction; D015533:Transcriptional Activation
C101326:RBP4 protein, human; D054839:Retinol-Binding Proteins, Plasma; C577981:PSMB1 protein, human; D046988:Proteasome Endopeptidase Complex