The fruits of Maclura pomifera extracts inhibits glioma stem-like cell growth and invasion.
Glioma is the most common primary intracranial tumour. Recently, growing evidence showed that glioma possesses stem-like cells, which are thought to be chemo- and radio-resistant and believed to contribute to the poor clinical outcomes of these tumours. In this study, we found that stem-like glioma cells (CD133+) were significantly increased in neurosphere cells, which are highly invasive and resistant to multiple chemotherapeutic agents. From our natural products library, we screened 48 natural products and found one compound, Pomiferin, which was of particular interest. Our results showed that Pomiferin could inhibit cell viability, CD133+ cell population, sphere formation, and invasion ability of glioma neurosphere cells. We also found that multiple stemness-associated genes (BIM1, Nestin, and Nanog) were down-regulated by Pomiferin treatment of glioma neurosphere cells. Taken together, our results suggest that Pomiferin could kill the cancer stem-like cells in glioma and may serve as a potential therapeutic agent in the future.
Department of Neurosurgery, Zhangjiagang Hospital, Suzhou University, Zhangjiagang, 215600, China.
Dan
D
Zhao
Chengyun
C
Yao
Xiaobing
X
Chen
Hongping
H
Xia
Li
L
Zhang
Huixiang
H
Liu
Xiaochun
X
Jiang
Yi
Y
Dai
Jun
J
Liu
D000071916:AC133 Antigen; D015703:Antigens, CD; D001578:Benzopyrans / Q000494:pharmacology / Q000627:therapeutic use*; D001932:Brain Neoplasms / Q000188:drug therapy; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D005638:Fruit / Q000737:chemistry; D005910:Glioma / Q000188:drug therapy* / Q000276:immunology; D006023:Glycoproteins; D006801:Humans; D007529:Isoflavones / Q000494:pharmacology / Q000627:therapeutic use*; D031624:Maclura / Q000737:chemistry*; D009361:Neoplasm Invasiveness / Q000517:prevention & control; D014411:Neoplastic Stem Cells / Q000187:drug effects; D010455:Peptides; D008517:Phytotherapy
D000071916:AC133 Antigen; D015703:Antigens, CD; D001578:Benzopyrans; D006023:Glycoproteins; D007529:Isoflavones; C000606023:PROM1 protein, human; D010455:Peptides; C474837:pomiferin