Human rhinovirus subviral a particle binds to lipid membranes over a twofold axis of icosahedral symmetry.
Minor group human rhinoviruses bind low-density lipoprotein (LDL) receptors for endocytosis. Once they are inside endosomes, the acidic pH triggers their dissociation from the receptors and conversion into hydrophobic subviral A particles; these attach to the membrane and transfer their single-strand, positive-sense RNA genome into the cytosol. Here, we allowed human rhinovirus 2 (HRV2) A particles, produced in vitro by incubation at pH 5.4, to attach to liposomes; cryo-electron microscopy 3-dimensional single-particle image reconstruction revealed that they bind to the membrane around a 2-fold icosahedral symmetry axis.
Max F. Perutz Laboratories, Department of Medical Biochemistry, Medical University of Vienna, Vienna, Austria.
Mohit
M
Kumar
Dieter
D
Blaas
D002462:Cell Membrane / Q000821:virology*; D020285:Cryoelectron Microscopy; D006801:Humans; D021621:Imaging, Three-Dimensional; D008081:Liposomes / Q000378:metabolism; D046911:Macromolecular Substances / Q000378:metabolism* / Q000648:ultrastructure; D008563:Membrane Lipids / Q000378:metabolism*; D012229:Rhinovirus / Q000502:physiology* / Q000648:ultrastructure; D053585:Virus Attachment
D008081:Liposomes; D046911:Macromolecular Substances; D008563:Membrane Lipids