Ectomycorrhizas with Paxillus involutus enhance cadmium uptake and tolerance in Populus × canescens.
Ectomycorrhizas (EMs), which are symbiotic organs formed between tree roots and certain fungi, can mediate cadmium (Cd) tolerance of host plants, but the underlying physiological and molecular mechanisms are not fully understood. To investigate EMs mediated Cd tolerance in woody plants, Populus × canescens was inoculated with Paxillus involutus (strain MAJ) to establish mycorrhizal roots. Mycorrhizal poplars and non-mycorrhizal controls were exposed to 0 or 50 μM CdSO4 . EMs displayed higher net Cd(2+) influx than non-mycorrhizal roots. Net Cd(2+) influx was coupled with net H(+) efflux and inactivation of plasma membrane (PM) H(+) -ATPases reduced Cd(2+) uptake of EMs less than of non-mycorrhizal roots. Consistent with higher Cd(2+) uptake in EMs, in most cases, transcript levels of genes involved in Cd(2+) uptake, transport and detoxification processes were increased in EMs compared to non-mycorrhizal roots. Higher CO2 assimilation, improved nutrient and carbohydrate status, and alleviated oxidative stress were found in mycorrhizal compared to non-mycorrhizal poplars despite higher Cd(2+) accumulation. These results indicate that mycorrhizas increase Cd(2+) uptake, probably by an enlarged root volume and overexpression of genes involved in Cd(2+) uptake and transport, and concurrently enhance Po. × canescens Cd tolerance by increased detoxification, improved nutrient and carbohydrate status and defence preparedness.
College of Forestry and State Key Laboratory of Crop Stress Biology for Arid Areas.
Yonglu
Y
Ma
Jiali
J
He
Chaofeng
C
Ma
Jie
J
Luo
Hong
H
Li
Tongxian
T
Liu
Andrea
A
Polle
Changhui
C
Peng
Zhi-Bin
ZB
Luo
D000222:Adaptation, Physiological / Q000235:genetics; D001487:Basidiomycota / Q000502:physiology*; D001692:Biological Transport / Q000235:genetics; D002104:Cadmium / Q000378:metabolism*; D050260:Carbohydrate Metabolism / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant / Q000235:genetics; D005978:Glutathione / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000378:metabolism; D008658:Inactivation, Metabolic; D008315:Malondialdehyde / Q000378:metabolism; D018519:Meristem / Q000382:microbiology; D008954:Models, Biological; D038821:Mycorrhizae / Q000502:physiology*; D018384:Oxidative Stress / Q000235:genetics; D024301:Plant Bark / Q000378:metabolism; D018515:Plant Leaves / Q000378:metabolism; D032107:Populus / Q000235:genetics / Q000254:growth & development / Q000378:metabolism* / Q000382:microbiology*; D025341:Principal Component Analysis; D011392:Proline / Q000378:metabolism; D011522:Protons; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017550:Spectroscopy, Fourier Transform Infrared; D013438:Sulfhydryl Compounds / Q000378:metabolism; D013481:Superoxides / Q000378:metabolism; D014934:Wood / Q000378:metabolism
D011522:Protons; D012333:RNA, Messenger; D013438:Sulfhydryl Compounds; D002104:Cadmium; D013481:Superoxides; D008315:Malondialdehyde; D011392:Proline; D006861:Hydrogen Peroxide; D005978:Glutathione
carbohydrates; gene expression; ion flux; mycorrhiza; nutrients; oxidative stress; plasma membrane proton-ATPases; poplar