Atomic force microscopy study of the effects of water-soluble fullerenes on the elasticity of living plant cells.
In this work, atomic force microscopy (AFM) was employed to characterize the elastic properties of a living suspension of Nicotiana tabacum L. cv. Bright Yellow (BY-2) cells and to investigate the changes in plant-cell elasticity that were induced by water-soluble C70 fullerene derivatives. The results revealed different effects of the three fullerene derivatives that had different numbers of carboxylic groups on the cell elasticity. BY-2 cells that were repressed by dimalonic-acid-modified C70 fullerenes (DiF70) and trimalonic-acid-modified C70 fullerenes (TriF70) showed a clear decrease in their Young's modulus. However, the Young's modulus of cells that were treated with tetramalonic-acid-modified C70 fullerenes (TetraF70) increased. Disruption of the actin cytoskeleton arrangement was observed following treatment with DiF70 and TriF70, but not with TetraF70. Moreover, the fullerene-induced cell-elasticity change was consistent with the change in cell-proliferation rate. This work provides a new approach and valuable information for the study of the biological effect of nanomaterials on plant cells.
Beijing National Laboratory for Molecular Sciences, Key Laboratory of Molecular Nanostructures and Nanotechnology, Institute of Chemistry, Chinese Academy of Sciences, 2 Zhongguancun Beiyijie, Beijing 100190 (P. R. China), Fax: (+86) 10-62653083.
Xuejie
X
Zhang
Qiaoling
Q
Liu
Tie
T
Xia
Nan
N
Li
Kangmin
K
He
Chunru
C
Wang
Weihong
W
Tan
Xiaohong
X
Fang
D055119:Elastic Modulus / Q000187:drug effects; D037741:Fullerenes / Q000737:chemistry* / Q000633:toxicity; D008314:Malonates / Q000737:chemistry; D018625:Microscopy, Atomic Force; D059828:Plant Cells / Q000187:drug effects; D014026:Nicotiana / Q000166:cytology / Q000187:drug effects*; D014867:Water / Q000737:chemistry
D037741:Fullerenes; D008314:Malonates; D014867:Water; C069836:fullerene C70; C030290:malonic acid
atomic force microscopy; cytotoxity; elasticity; fullerenes; nanoparticles