Efficient detection of hydrogen bonds in dynamic regions of RNA by sensitivity-optimized NMR pulse sequences.
Improved Sensitivity: Efficient NMR experiments are presented for determining the secondary structure in large and dynamic RNAs using J-couplings across hydrogen bonds. The experiments provide up to eight-fold improved sensitivity and thus enable detection of base pairs in dynamic regions even in large RNAs.
Institute of Structural Biology, Helmholtz Zentrum München, Ingolstädter Landstr. 1, 85764 Neuherberg (Germany) http://www.nmr.ch.tum.de/; Center for Integrated Protein Science Munich and Chair of Biomolecular NMR, TU München, Lichtenbergstr. 4, 85747 Garching (Germany).
Andre
A
Dallmann
Bernd
B
Simon
Malgorzata M
MM
Duszczyk
Hamed
H
Kooshapur
Arthur
A
Pardi
Wolfgang
W
Bermel
Michael
M
Sattler
D001483:Base Sequence; D006860:Hydrogen Bonding; D008969:Molecular Sequence Data; D008970:Molecular Weight; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D009690:Nucleic Acid Conformation; D012313:RNA / Q000737:chemistry*
D012313:RNA
NMR spectroscopy; RNA dynamics; RNA secondary structure; base pairs; hydrogen bonds