Combined effects of dietary fructooligosaccharide and Bacillus licheniformis on innate immunity, antioxidant capability and disease resistance of triangular bream (Megalobrama terminalis).
This study was conducted to investigate the effects of fructooligosaccharide (FOS) and Bacillus licheniformis (B. licheniformis) and their interaction on innate immunity, antioxidant capability and disease resistance of triangular bream Megalobrama terminalis (average initial weight 30.5 ± 0.5 g). Nine experimental diets were formulated to contain three FOS levels (0, 0.3% and 0.6%) and three B. licheniformis levels (0, 1 × 10(7), 5 × 10(7) CFU g(-1)) according to a 3 × 3 factorial design. At the end of the 8-week feeding trial, fish were challenged by Aeromonas hydrophila (A. hydrophila) and survival rate was recorded for the next 7 days. The results showed that leucocyte counts, alternative complement activity as well as total serum protein and globulin contents all increased significantly (P < 0.05) as dietary B. licheniformis levels increased from 0 to 1 × 10(7) CFU g(-1), while little difference (P > 0.05) was observed in these parameters in terms of dietary FOS levels. Both plasma alkaline phosphatase and phenoloxidase activities were significantly (P < 0.05) affected only by dietary FOS levels with the highest values observed in fish fed 0.6 and 0.3% FOS, respectively. Both immunoglobulin M content and liver superoxide dismutase (SOD) activity were significantly affected (P > 0.05) by both FOS and B. licheniformis. Liver catalase, glutathione peroxidase as well as plasma SOD activities of fish fed 1 × 10(7) CFU g(-1)B. licheniformis were all significantly (P < 0.05) higher than that of the other groups, whereas the opposite was true for malondialdehyde content. After A. hydrophila challenge, survival rate was not affected (P > 0.05) by either FOS levels or B. licheniformis contents, whereas a significant (P < 0.05) interaction between these two substances was observed with the highest value observed in fish fed 0.3% FOS and 1 × 10(7) CFU g(-1)B. licheniformis. The results of this study indicated that dietary FOS and B. licheniformis could significantly enhance the innate immunity and antioxidant capability of triangular bream, as well as improve its disease resistance. The best combination of these two prebiotics and/or probiotics was 0.3% FOS and 1 × 10(7) CFU g(-1)B. licheniformis.
Key Laboratory of Aquatic Nutrition and Feed Science of Jiangsu Province, College of Animal Science and Technology, Nanjing Agricultural University, No. 1 Weigang Road, Nanjing 210095, PR China.
Chun-Nuan
CN
Zhang
Xiang-Fei
XF
Li
Wei-Na
WN
Xu
Guang-Zhen
GZ
Jiang
Kang-Le
KL
Lu
Li-Na
LN
Wang
Wen-Bin
WB
Liu
D000135:Acid Phosphatase / Q000378:metabolism; D016980:Aeromonas hydrophila / Q000276:immunology; D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D017756:Aquaculture / Q000379:methods; D001407:Bacillus / Q000276:immunology*; D003530:Cyprinidae / Q000276:immunology* / Q000378:metabolism / Q000382:microbiology; D004040:Dietary Carbohydrates / Q000494:pharmacology*; D060467:Disease Resistance / Q000187:drug effects / Q000276:immunology*; D004305:Dose-Response Relationship, Drug; D007113:Immunity, Innate / Q000187:drug effects / Q000276:immunology*; D016014:Linear Models; D009844:Oligosaccharides / Q000494:pharmacology; D016019:Survival Analysis
D000975:Antioxidants; D004040:Dietary Carbohydrates; D009844:Oligosaccharides; C116580:fructooligosaccharide; D000135:Acid Phosphatase
Antioxidant capability; Disease resistance; Fructooligosaccharide; Innate immunity; Megalobrama terminalis