Increased Th22 cells are independently associated with Th17 cells in type 1 diabetes.
Type 1 diabetes (T1D) is perceived as an autoimmune disease caused by T cell-mediated destruction of the insulin-producing pancreatic β cells. However, the number of inflammatory T cells in blood, as well as the relative importance of each cell type is unclear. Forty-two patients with T1D and 30 controls were enrolled. Circulating primary CD4(+) or CD8(+) T cells were quantified with 5-color flow cytometry. Serum IL-22 and IL-17 levels were examined by ELISA. Serum autoantibodies were measured by radio-binding assays, using (35)S-labeled glutamic acid decarboxylase-65 (GAD65), protein tyrosine phosphatase-2 (IA-2), and zinc transporter 8 (ZnT8). Th17-Th22 and Tc1-Tc17 were significantly elevated in patients with T1D compared to control subjects, while there were no significant differences in Th1 cells. The levels of these T cells in different stages of T1D were investigated. Th22 cells showed a positive correlation with Th17 cells in T1D patients. However, we did not find any correlation between IL-17 and IL-22 in sera. Autoantibodies were not significantly different between patients with early T1D and those who have had it for a longer duration. This study indicates that Th22 may contribute to the pathogenesis of T1D. Blockade of Th22 cells might be of clinical profit in T1D patients.
Department of Endocrinology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, 300 Guangzhou Road, Nanjing, 210029, Jiangsu, China.
Xinyu
X
Xu
Shuai
S
Zheng
Fan
F
Yang
Yun
Y
Shi
Yong
Y
Gu
Heng
H
Chen
Mei
M
Zhang
Tao
T
Yang
D000328:Adult; D001323:Autoantibodies / Q000032:analysis; D015496:CD4-Positive T-Lymphocytes / Q000473:pathology; D018414:CD8-Positive T-Lymphocytes / Q000473:pathology; D003922:Diabetes Mellitus, Type 1 / Q000473:pathology*; D005260:Female; D006801:Humans; D007378:Interleukins / Q000378:metabolism*; D007515:Islets of Langerhans / Q000276:immunology; D018655:Lymphocyte Count; D008297:Male; D013601:T-Lymphocytes / Q000378:metabolism / Q000473:pathology*; D013602:T-Lymphocytes, Cytotoxic / Q000473:pathology; D018417:Th1 Cells / Q000473:pathology; D058504:Th17 Cells / Q000378:metabolism / Q000473:pathology*; D055815:Young Adult; D000097043:Interleukin-22
D001323:Autoantibodies; D007378:Interleukins; D000097043:Interleukin-22