Fast flexible multiphoton fluorescence lifetime imaging using acousto-optic deflector.
We present a fast and flexible fluorescence lifetime imaging microscopy which uses a two-dimensional acousto-optic deflector to achieve fast beam scanning across the sample and provides random access to the regions of interests (ROI). Experimental results using standard fluorescent dye and biological samples show that this system can make addressable fluorescence lifetime measurements and perform fast and flexible fluorescence lifetime imaging particularly to the discontinuous ROI in the sample.
Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Optoelectronic Engineering, Shenzhen University, Shenzhen, China.
Jing
J
Qi
Yonghong
Y
Shao
Lixin
L
Liu
Kaige
K
Wang
Tongsheng
T
Chen
Junle
J
Qu
Hanben
H
Niu
D000162:Acoustics / Q000295:instrumentation*; D000818:Animals; D051379:Mice; D036641:Microscopy, Fluorescence, Multiphoton / Q000295:instrumentation*; D055096:Optical Devices