The expression and release of Hsp60 in 6-OHDA induced in vivo and in vitro models of Parkinson's disease.
In Parkinson's disease, dopaminergic neuron damage/death causes the release of soluble substances that are selectively toxic to neighboring/additional dopaminergic neurons through the activation of microglia. Hsp60 can be released from injured cells of central nervous system to activate microglia. However, its expression and role in Parkinson's disease has not been well understood. Here, we performed a 6-OHDA treated Parkinson's disease model in adult rats. Western blot analysis showed a time-course expression of Hsp60, which decreased gradually and then rose back. Immunofluorescence staining showed that Hsp60 was decreased in dopaminergic neuron, and most Hsp60 located on the surface of activated microglia. Furthermore, in cellular Parkinson's disease model, Hsp60 was obviously detected in the culture supernatants after 6-OHDA treatment, and a concomitant decrease in cell extracts. Taken together, our results suggested that Hsp60 could be released extracellularly to activate microglia in Parkinson's disease model.
Department of Geriatrics, The Second Affiliated Hospital, Nanjing Medical University, Nanjing, 210011, Jiangsu, People's Republic of China, mjfeng416@yahoo.com.cn.
Mei jiang
Mj
Feng
Ling
L
Zhang
Zhengxia
Z
Liu
Ping
P
Zhou
Xiang
X
Lu
D000818:Animals; D018834:Chaperonin 60 / Q000096:biosynthesis* / Q000378:metabolism; D004195:Disease Models, Animal; D059290:Dopaminergic Neurons / Q000378:metabolism; D008297:Male; D017628:Microglia / Q000187:drug effects; D024101:Mitochondrial Proteins / Q000096:biosynthesis* / Q000378:metabolism; D016627:Oxidopamine; D016716:PC12 Cells; D010300:Parkinson Disease / Q000503:physiopathology*; D051381:Rats
D018834:Chaperonin 60; C557067:Hspd1 protein, rat; D024101:Mitochondrial Proteins; D016627:Oxidopamine