Influence of bovine sperm DNA fragmentation and oxidative stress on early embryo in vitro development outcome.
Sperm chromatin fragmentation may be caused by a number of factors, the most significant of which is reactive oxygen species. However, little is known about the effect of sperm oxidative stress (OS) on DNA integrity, fertilization, and embryonic development in cattle. Therefore, the goal of this study was to evaluate the influence of sperm OS susceptibility on the DNA fragmentation rate and in vitro embryo production (IVP) in a population of bulls. Groups of cryopreserved sperm samples were divided into four groups, based on their susceptibility to OS (G1, low OS; G2, average OS; G3, high OS; and G4, highest OS). Our results demonstrated that the sperm DNA integrity was compromised in response to increased OS susceptibility. Furthermore, semen samples with lower susceptibility to OS were also less susceptible to DNA damage (G1, 4.06%; G2, 6.09%; G3, 6.19%; and G4, 6.20%). In addition, embryo IVP provided evidence that the embryo cleavage rate decreased as the OS increased (G1, 70.18%; G2, 62.24%; G3, 55.85%; and G4, 50.93%), but no significant difference in the blastocyst rate or the number of blastomeres was observed among the groups. The groups with greater sensitivity to OS were also associated with a greater percentage of apoptotic cells (G1, 2.6%; G2, 2.76%; G3, 5.59%; and G4, 4.49%). In conclusion, we demonstrated that an increased susceptibility to OS compromises sperm DNA integrity and consequently reduces embryo quality.
Centro de Ciências Naturais e Humanas (CCNH), Federal University of ABC, Rua Santa Adélia, 166, Bairro Bangu, Santo André, SP CEP 09210-170, Brazil.
Renata
R
Simões
Weber Beringui
WB
Feitosa
Adriano Felipe Perez
AF
Siqueira
Marcilio
M
Nichi
Fabíola Freitas
FF
Paula-Lopes
Mariana Groke
MG
Marques
Maria Angélica
MA
Peres
Valquíria Hyppolito
VH
Barnabe
José Antônio
JA
Visintin
Mayra Elena Ortiz
ME
Assumpção
D000003:Abattoirs; D000818:Animals; D017209:Apoptosis; D001755:Blastocyst / Q000166:cytology / Q000378:metabolism; D001757:Blastomeres / Q000166:cytology / Q000378:metabolism; D002417:Cattle / Q000502:physiology*; D002970:Cleavage Stage, Ovum / Q000166:cytology / Q000378:metabolism; D015925:Cryopreservation / Q000662:veterinary; D053938:DNA Fragmentation; D004477:Ectogenesis; D005260:Female; D005307:Fertilization in Vitro / Q000662:veterinary*; D059471:In Vitro Oocyte Maturation Techniques / Q000662:veterinary; D007700:Kinetics; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D018384:Oxidative Stress; D055101:Semen Analysis / Q000662:veterinary; D013094:Spermatozoa / Q000166:cytology / Q000378:metabolism*; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
D017392:Thiobarbituric Acid Reactive Substances; D008315:Malondialdehyde