Optical clearing-aided photoacoustic microscopy with enhanced resolution and imaging depth.
Due to strong light scattering in tissue, both the spatial resolution and maximum penetration depth of optical-resolution photoacoustic microscopy (OR-PAM) deteriorate sharply with depth. To reduce tissue scattering, we propose to use glycerol as an optical clearing agent in OR-PAM. Our results show that the imaging performance of OR-PAM can be greatly enhanced by optical clearing both in vitro and in vivo.
Optical Imaging Laboratory, Department of Biomedical Engineering, Washington University in St. Louis, St. Louis, Missouri 63130, USA.
Yong
Y
Zhou
Junjie
J
Yao
Lihong V
LV
Wang
D000818:Animals; D000078404:Epidermal Cells; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D051379:Mice; D008853:Microscopy / Q000379:methods*; D055620:Optical Phenomena; D019047:Phantoms, Imaging; D061088:Photoacoustic Techniques / Q000379:methods*; D059629:Signal-To-Noise Ratio