Co-infection with Arsenophonus nasoniae and Orientia tsutsugamushi in a traveler.
Here we report a case of co-infection with Orientia tsutsugamushi, the causative agent of scrub typhus, and Arsenophonus nasoniae in a woman with a rash and an eschar who returned from a trip to Southeast Asia. A. nasoniae was previously considered to be a secondary insect and tick endosymbiont of unknown pathogenicity in humans. We amplified both O. tsutsugamushi and A. nasoniae DNA from a skin eschar with qPCR, and a seroconversion for O. tsutsugamushi and A. nasoniae was observed with immunofluorescence assays and western blotting for this patient. And we used 2-D western blotting with an A. nasoniae antigen and polyclonal mouse anti-A. nasoniae antibodies produced in our laboratory to detect the specific antigenic A. nasoniae proteins.
Aix Marseille Université, URMITE, UM63, CNRS 7278, IRD 198, Inserm 1095, Marseille, France.
Sophie
S
Edouard
Geetha
G
Subramanian
Benjamin
B
Lefevre
Anabelle
A
Dos Santos
Pascal
P
Pouedras
Yves
Y
Poinsignon
Oleg
O
Mediannikov
Didier
D
Raoult
D000328:Adult; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood* / Q000276:immunology; D000942:Antigens, Bacterial / Q000276:immunology / Q000302:isolation & purification; D001210:Asia, Southeastern; D001426:Bacterial Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D001706:Biopsy; D060085:Coinfection; D004755:Enterobacteriaceae / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D004756:Enterobacteriaceae Infections / Q000150:complications* / Q000175:diagnosis / Q000382:microbiology; D005260:Female; D005602:France; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D012285:Orientia tsutsugamushi / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction; D012612:Scrub Typhus / Q000150:complications* / Q000175:diagnosis / Q000382:microbiology; D012867:Skin / Q000382:microbiology* / Q000473:pathology; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014195:Travel
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001426:Bacterial Proteins