Increased serum oxidative stress markers in women with uterine leiomyoma.
BACKGROUND
Uterine leiomyomas (fibroids) are the most common gynaecological benign tumors in premenopausal women. Evidences support the role of oxidative stress in the development of uterine leiomyoma. We have analysed oxidative stress markers (thiols, advanced oxidized protein products (AOPP), protein carbonyls and nitrates/nitrites) in preoperative sera from women with histologically proven uterine leiomyoma.


RESULTS
We conducted a laboratory study in a tertiary-care university hospital. Fifty-nine women with histologically proven uterine leiomyoma and ninety-two leiomyoma-free control women have been enrolled in this study. Complete surgical exploration of the abdominopelvic cavity was performed in each patient. Preoperative serum samples were obtained from all study participants to assay serum thiols, AOPP, protein carbonyls and nitrates/nitrites. Concentrations of serum protein carbonyl groups and AOPP were higher in leiomyoma patients than in the control group (p=0.005 and p<0.001, respectively). By contrast, serum thiol levels were lower in leiomyoma patients (p<0.001). We found positive correlations between serum AOPP concentrations and total fibroids weight (r=0.339; p=0.028), serum AOPP and serum protein carbonyls with duration of infertility (r=0.762; p=0.006 and r=0.683; p=0.021, respectively).


CONCLUSIONS
This study, for the first time, reveals a significant increase of protein oxidative stress status and reduced antioxidant capacity in sera from women with uterine leiomyoma.
Université Paris Descartes, Sorbonne Paris Cité,Faculté de Médecine, AP- HP, Hôpital Cochin, Department of GynecologyObstetrics II and Reproductive Medicine,75679 Paris, France. pietro.santulli@cch.aphp.fr
Pietro
P
Santulli
Bruno
B
Borghese
Herve
H
Lemaréchal
Mahaut
M
Leconte
Anne-Elodie
AE
Millischer
Frédéric
F
Batteux
Charles
C
Chapron
Didier
D
Borderie
D000328:Adult; D062107:Advanced Oxidation Protein Products / Q000097:blood*; D015415:Biomarkers / Q000097:blood; D001798:Blood Proteins / Q000378:metabolism; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D007889:Leiomyoma / Q000097:blood* / Q000175:diagnosis / Q000473:pathology; D008875:Middle Aged; D009566:Nitrates / Q000097:blood; D009573:Nitrites / Q000097:blood; D018384:Oxidative Stress; D017697:Premenopause / Q000097:blood; D050050:Protein Carbonylation; D013438:Sulfhydryl Compounds / Q000097:blood; D014594:Uterine Neoplasms / Q000097:blood* / Q000175:diagnosis / Q000473:pathology
D062107:Advanced Oxidation Protein Products; D015415:Biomarkers; D001798:Blood Proteins; D009566:Nitrates; D009573:Nitrites; D013438:Sulfhydryl Compounds