[The efficacy and safety of unmanipulated haploidentical peripheral blood stem cell transplantation on hematologic diseases].
OBJECTIVE
To evaluate the efficacy and safety of unmanipulated haploidentical allogeneic peripheral blood stem cells transplantation (PBSCT) on hematologic diseases.


METHODS
Patients who underwent unmanipulated HLA-mismatched/haploidentical PBSCT from July 2007 to December 2011 were investigated retrospectively.


RESULTS
Forty-nine patients with hematologic diseases underwent unmanipulated human leukocyte antigen (HLA)-mismatched/haploidentical PBSCT with myeloablative conditioning. All patients were mismatched at the allele level for HLA-A, HLA-B, HLA-Cw, HLA-DRB1 and HLA-DQ1. Fifteen patients were mismatched in 5 loci, 11 patients in 4 loci, 7 patients in 3 loci, 5 patients in 2 loci, and 11 patients in 1 locus. The median numbers of mononuclear cells and CD₃₄⁺ cells infused at transplantation were 10.01(7.05-25.34) × 10⁸/kg and 4.51 (2.01-11.47) × 10⁶/kg, respectively. Patients achieved myeloid and platelet engraftment at a median of 14 (10-25) days and 22 (10-135) days, respectively. The cumulative incidence of acute graft versus host disease (aGVHD) on day 100 was (61.6 ± 7.3)%, and the 2-year cumulative incidence of chronic graft versus host disease (cGVHD) was (42.6 ± 8.5)%. One hundred-day transplantation related mortality (TRM) rate and 2-year cumulative TRM rate were (14.7 ± 5.1)% and (30.9 ± 8.8)%, respectively. The 2-year cumulative incidence estimate of relapse was (25.4 ± 7.0)%. The 2-year cumulative overall survival rate was (58.1 ± 8.8)% and 2-year disease-free survival rate was (53.9 ± 8.4)% with an 11.5-months median follow-up.


CONCLUSIONS
Unmanipulated PBSCT is a promising protocol for patients with hematologic diseases in HLA-mismatched/haploidentical transplant settings.
Department of Hematology, Chinese PLA General Hospital, Beijing 100853, China.
Wen-rong
WR
Huang
Hong-hua
HH
Li
Xiang-shu
XS
Jin
Shu-hong
SH
Wang
Jian
J
Bo
Yu
Y
Zhao
Yu
Y
Jing
Hai-yan
HY
Zhu
Li-ping
LP
Dou
Bo-jun
BJ
Jia
Qun-shun
QS
Wang
Chun-ji
CJ
Gao
Li
L
Yu
D000293:Adolescent; D000328:Adult; D002648:Child; D002675:Child, Preschool; D005260:Female; D006680:HLA Antigens / Q000276:immunology; D006238:Haploidy; D006402:Hematologic Diseases / Q000276:immunology / Q000601:surgery*; D006648:Histocompatibility; D006801:Humans; D008297:Male; D008875:Middle Aged; D036102:Peripheral Blood Stem Cell Transplantation / Q000379:methods*; D012189:Retrospective Studies; D019172:Transplantation Conditioning; D016896:Treatment Outcome; D055815:Young Adult
D006680:HLA Antigens