Physiological control of bioluminescence in a deep-sea planktonic worm, Tomopteris helgolandica.
Tomopteris helgolandica Greeff 1879 (Tomopteridae) is a transparent holoplanktonic polychaete that can emit a bright light. In this study, we investigated the emission pattern and control of this deep-sea worm's luminescence. Potassium chloride depolarisation applied on anaesthetised specimens triggered a maximal yellow light emission from specific parapodial sites, suggesting that a nervous control pathway was involved. Pharmacological screening revealed a sensitivity to carbachol, which was confirmed by a dose-light response associated with a change in the light emission pattern, where physiological carbachol concentrations induced flashes and higher concentrations induced glows. The light response induced by its hydrolysable agonist, acetylcholine, was significantly weaker but was facilitated by eserine pretreatment. In addition, a specific inhibitory effect of tubocurarine was observed on carbachol-induced emission. Lastly, KCl- and carbachol-induced light responses were significantly reduced when preparations were pre-incubated in Ca(2+)-free artificial seawater or in different calcium channel blockers (verapamil, diltiazem) and calmodulin inhibitor (trifluoperazine) solutions. All of these results strongly suggest that T. helgolandica produces its light flashes via activation of nicotinic cholinergic receptors and a calcium-dependent intracellular mechanism involving L-type calcium channels.
Marine Biology Laboratory, Earth and Life Institute, Catholic University of Louvain, Place Croix du Sud 3, Louvain-la-Neuve 1348, Belgium.
Anaïd
A
Gouveneaux
Jérôme
J
Mallefet
D000109:Acetylcholine / Q000494:pharmacology; D000704:Analysis of Variance; D000818:Animals; D001267:Atlantic Ocean; D002217:Carbachol / Q000819:agonists / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D008163:Luminescent Measurements; D009664:Norway; D011077:Polychaeta / Q000502:physiology*; D011189:Potassium Chloride / Q000378:metabolism*; D011978:Receptors, Nicotinic / Q000378:metabolism*; D012623:Seawater / Q000737:chemistry; D014403:Tubocurarine / Q000494:pharmacology; D015048:Zooplankton / Q000502:physiology*
D011978:Receptors, Nicotinic; D011189:Potassium Chloride; D002217:Carbachol; D000109:Acetylcholine; D014403:Tubocurarine
Tomopteridae; annelid; cholinergic control; nervous control; plankton; yellow light