Advanced techniques for detection and identification of microbial agents of gastroenteritis.
Gastroenteritis persists as a worldwide problem, responsible for approximately 2 million deaths annually. Traditional diagnostic methods used in the clinical microbiology laboratory include a myriad of tests, such as culture, microscopy, and immunodiagnostics, which can be labor intensive and suffer from long turnaround times and, in some cases, poor sensitivity. [corrected]. This article reviews recent advances in genomic and proteomic technologies that have been applied to the detection and identification of gastrointestinal pathogens. These methods simplify and speed up the detection of pathogenic microorganisms, and their implementation in the clinical microbiology laboratory has potential to revolutionize the diagnosis of gastroenteritis.
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Sherry A
SA
Dunbar
Hongwei
H
Zhang
Yi-Wei
YW
Tang
D016360:Clostridioides difficile / Q000235:genetics* / Q000302:isolation & purification; D005243:Feces / Q000382:microbiology; D005759:Gastroenteritis / Q000175:diagnosis* / Q000382:microbiology; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D040901:Proteomics / Q000379:methods; D017422:Sequence Analysis, DNA
Clostridium difficile; Gastroenteritis; Multiplex polymerase chain reaction; Next-generation sequencing; Suspension array