Space-division multiplexing optical coherence tomography.
High speed, high resolution and high sensitivity are desirable for optical coherence tomography (OCT). Here, we demonstrate a space-division multiplexing (SDM) technology that translates long coherence length of a commercially available wavelength tunable laser into high OCT imaging speed. We achieved an effective 800,000 A-scans/s imaging speed using a 100,000 Hz tunable vertical cavity surface-emitting laser (VCSEL). A sensitivity of 94.6 dB and a roll-off of < 2 dB over ~30 mm imaging depth were measured from a single channel in the prototype SDM-OCT system. An axial resolution of ~11 μm in air (or ~8.3 μm in tissue) was achieved throughout the entire depth range. An in vivo, 3D SDM-OCT volume of an entire Drosophila larva consisting of 400 x 605 A-scans was acquired in 0.37 seconds. Synchronized cross-sectional OCT imaging of three different segments of a beating Drosophila larva heart is demonstrated. The SDM technology provides a new orthogonal dimension for further speed improvement for OCT with favorable cost scaling. SDM-OCT also preserves image resolution and allows synchronized cross-sectional and three-dimensional (3D) imaging of biological samples, enabling new biomedical applications.
Department of Electrical and Computer Engineering, Lehigh University, Bethlehem, Pennsylvania 18015, USA. chaozhou@lehigh.edu
Chao
C
Zhou
Aneesh
A
Alex
Janarthanan
J
Rasakanthan
Yutao
Y
Ma
D000818:Animals; D004331:Drosophila melanogaster / Q000033:anatomy & histology; D006321:Heart / Q000033:anatomy & histology; D007814:Larva / Q000033:anatomy & histology; D041623:Tomography, Optical Coherence / Q000379:methods*