Characterization of the bacterioplankton community and its antibiotic resistance genes in the Baltic Sea.
The residues from human environments often contain antibiotics and antibiotic resistance genes (ARGs) that can contaminate natural environments; the clearest consequence of that is the selection of antibiotic-resistant bacteria. The Baltic Sea is the second largest isolated brackish water reservoir on Earth, serving as a drainage area for people in 14 countries, which differ from one another in antibiotic use and sewage treatment policies. The aim of this study was to characterize the bacterioplankton structure and quantify ARGs (tetA, tetB, tetM, ermB, sul1, blaSHV, and ampC) within the bacterioplankton community of the Baltic Sea. Quantitative polymerase chain reaction was applied to quantify ARGs from four different sampling sites of the Baltic Sea over 2 years, and the bacterial communities were profiled sequencing the V6 region of the 16S rRNA gene on Illumina HiSeq2000. The results revealed that all the resistance genes targeted in the study were detectable from the Baltic Sea bacterioplankton. The percentage of tetA, tetB, tetM, ermB, and sul1 genes in the sea bacterial community varied between 0.0077% and 0.1089%, 0.0003% and 0.0019%, 0.0001% and 0.0105%, 0% and 0.0136%, and 0.0001% and 0.0438%, respectively. The most numerous ARG detected was the tetA gene and this gene also had the highest proportion in the whole microbial community. A strong association between bacterioplankton ARGs' abundance data and community phylogenetic composition was found, implying that the abundance of most of the studied ARGs in the Baltic Sea is determined by fluctuations in its bacterial community structure.
Institute of Ecology and Earth Sciences, Faculty of Science and Technology, University of Tartu, Tartu, Estonia.
Kertu
K
Tiirik
Hiie
H
Nõlvak
Kristjan
K
Oopkaup
Marika
M
Truu
Jens-Konrad
JK
Preem
Ain
A
Heinaru
Jaak
J
Truu
D001419:Bacteria / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification*; D004352:Drug Resistance, Microbial / Q000235:genetics*; D005798:Genes, Bacterial / Q000235:genetics*; D009792:Oceans and Seas; D010933:Plankton / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics
D012336:RNA, Ribosomal, 16S
Baltic Sea; antibiotic resistance genes; next-generation sequencing; qPCR