Reprint of "new complete structure of Hafnia alvei clinical isolate strain PCM 2670 semi-rough lipopolysaccharide".
Hafnia alvei strain PCM 2670 is a clinical isolate from a patient with chronic reproductive tract infection. The novel structure of the semi-rough lipopolysaccharide was established with the use of NMR spectroscopy and mass spectrometry as well as immunochemical techniques. According to the mass spectrometry data, heptose in the oligosaccharide is partially substituted by glycine. H. alvei PCM 2670 core structure encompasses the common core of H. alvei which is modified with two additional galactose units. [formula see text] The 6-substituted galactose is the O-antigen repeating unit substitution residue. The repeating unit consists of five monosaccharide residues and has the following structure: →2)-β-Galp-(1→6)-α-Glcp-(1→6)-αGlcpNAc3OAc-(1→4)-α-GalpA-(1→3)-β-GlcpNAc6OAc-(1→6)-core.
Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, R. Weigla 12, PL-53-114 Wroclaw, Poland.
Ewelina
E
Bobko
Michal
M
Tyras
Wojciech
W
Jachymek
D002240:Carbohydrate Sequence; D005998:Glycine; D020611:Hafnia alvei / Q000737:chemistry*; D006801:Humans; D008050:Lipid A / Q000737:chemistry; D008070:Lipopolysaccharides / Q000737:chemistry*; D008969:Molecular Sequence Data
D008050:Lipid A; D008070:Lipopolysaccharides; D005998:Glycine
ATCC; American Type Culture Collection; DEPT; ESI-(Q)-TOF; ESIMS; GC–MS; Glycine; H. alvei common core oligosaccharide tetanus toxoid conjugate; H. alvei–OS-TT; HMBC; HSQC; Hafnia alvei; MALDI-TOFMS; MS; MS(2); NMR; NOESY; Nuclear Overhauser Effect spectroscopy; OS; P-PEtN; PCM; PS; Polish Collection of Microorganisms; SDS–PAGE; SR; Semi-rough lipopolysaccharide; TOCSY; distortionless enhancement by polarization transfer; double quantum filtered correlation spectroscopy; dqf COSY; electrospray ionization mass spectrometry; electrospray ionization quadrupole time of flight; gas chromatography–mass spectrometry; heteronuclear multiple bond correlation; heteronuclear single quantum coherence; mass spectrometry; matrix assisted laser desorption/ionization time of flight mass spectrometry; nuclear magnetic resonance; oligosaccharide; polysaccharide; pyrophosphoethanolamine; semi-rough; sodium dodecyl sulfate–polyacrylamide gel electrophoresis; tandem mass spectrometry; total correlation spectroscopy