Comparing brain-derived neurotrophic factor and ciliary neurotrophic factor secretion of induced neurotrophic factor secreting cells from human adipose and bone marrow-derived stem cells.
Adipose derived stem cells (ADSCs) and bone marrow stem cells (BMSCs) may be equally beneficial in treating neurodegenerative diseases. However, ADSCs have practical advantages. In this study, we aimed to induce neurotrophic factors secreting cells in human ADSCs. Then, we compared the level of brain-derived neurotrophic factor (BDNF) and ciliary neurotrophic factor (CNTF) secretion in neurotrophic factors secreting cells from human adipose and bone marrow-derived stem cells. Isolated human ADSCs and BMSCs were induced to neurotrophic factor (NTF)-secreting cells. The levels of expression and secretion of BDNF and CTNF of induced cells were assessed using immunocytochemical, Real-Time polymerase chain reaction, and enzyme linked immunosorbent assay (ELISA). The level of BDNF significantly increased in both the induced mesenchymal stem cells (MSCs) relative to ADSCs and the BMSCs (P < 0.01). Moreover, ELISA analysis showed that the release of BDNF in the induced BMSCs was almost twofold more than the induced ADSCs. Overall, NTF-secreting factor cells derived BMSCs and ADSCs could secret a range of different growth factors. Therefore, the variation in neurotrophic factors of different induced MSC populations suggest the possible beneficial effect of each specific kind of neurotrophic factor secreting cells for the treatment of a particular neurodegenerative disease.
Department of Anatomical Sciences and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran. razavi@med.mui.ac.ir
Shahnaz
S
Razavi
Mohamad Reza
MR
Razavi
Hamid
H
Zarkesh Esfahani
Mohammad
M
Kazemi
Fatemeh Sadat
FS
Mostafavi
D000273:Adipose Tissue / Q000166:cytology / Q000378:metabolism*; D001854:Bone Marrow Cells / Q000166:cytology*; D019208:Brain-Derived Neurotrophic Factor / Q000378:metabolism*; D020934:Ciliary Neurotrophic Factor / Q000378:metabolism*; D006801:Humans; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism*
D019208:Brain-Derived Neurotrophic Factor; D020934:Ciliary Neurotrophic Factor
bone marrow stem cells; human adipose-derived stem cells; neurodegenerative; neurotrophic factor secreting cell