Classification of type 2 diabetes rats based on urine amino acids metabolic profiling by liquid chromatography coupled with tandem mass spectrometry.
An analytical method for quantifying underivatized amino acids (AAs) in urine samples of rats was developed by using liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS). Classification of type 2 diabetes rats was based on urine amino acids metabolic profiling. LC-MS/MS analysis was applied through chromatographic separation and multiple reactions monitoring (MRM) transitions of MS/MS. Multivariate profile-wide predictive models were constructed using partial least squares discriminant analysis (PLS-DA) by SIMAC-P 11.5 version software package and hierarchical cluster analysis (HCA) by SPSS 18.0 version software. Some amino acids in urine of rats have significant change. The results of the present study prove that this method could perform the quantification of free AAs in urine of rats by using LC-MS/MS. In summary, the PLS-DA and HCA statistical analysis in our research were preferable to differentiate healthy rats and type 2 diabetes rats by the quantification of AAs in their urine samples. In addition, comparing with health group the seven increased amino acids in urine of type 2 rats were returned to normal under the treatment of acarbose.
Chemical Biology Laboratory & Changchun Center of Mass Spectrometry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China.
Chunyan
C
Wang
Hongbin
H
Zhu
Zifeng
Z
Pi
Fengrui
F
Song
Zhiqiang
Z
Liu
Shuying
S
Liu
D000596:Amino Acids / Q000652:urine*; D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D016000:Cluster Analysis; D003921:Diabetes Mellitus, Experimental / Q000145:classification / Q000378:metabolism / Q000652:urine*; D003924:Diabetes Mellitus, Type 2 / Q000145:classification / Q000378:metabolism / Q000652:urine*; D016018:Least-Squares Analysis; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry / Q000379:methods*
D000596:Amino Acids
LC–MS/MS; Rat urine; Type 2 diabetes; Underivatized amino acid