Nanostructural response of mitomycin C application on human scleral tissues.
This study quantitatively examined short-term effects of 0.02% Mitomycin C (MMC) treatment on the nanostructural changes in human scleral collagen fibrils. Histologic analysis and non-contact mode atomic force microscopy (AFM) were employed to assess the ultrastructural changes in the morphological characteristics of human sclera before and after 0.02% MMC application for 1 and 3 min. The scleral collagen fibrils treated with 0.02% MMC for 1 min showed no significant change in the morphology of collagen fibrils, and a significant change (p < 0.05) in the thickness of scleral tissues and collagen density, compared to the controls. 0.02% MMC application for 3 min led to a significant increase (p < 0.001) in the mean fibril diameter (185.43 +/- 22.64 nm vs. 140.72 +/- 18.06 nm), thickness (0.81 +/- 0.03 mm vs. 0.54 +/- 0.05 mm) and collagen density (1.16 times), compared to the controls This study examined the nanostructural changes in the scleral collagen fibrils before and after MMC application by AFM technique combined with conventional histological analysis (Hematoxylin-eosin and Masson's trichrome). This result indirectly suggests that long-term MMC application might increase the incidence of complications like a scleromalcia.
Department of Biomedical Engineering and Healthcare Industry Research Institute, College of Medicine, Kyung Hee University, Seoul 130-701, Republic of Korea.
Samjin
S
Choi
Seung-Chan
SC
Lee
Youjin
Y
Cheong
Jae-Ho
JH
Shin
Kyung-Hyun
KH
Jin
Hun-Kuk
HK
Park
D000328:Adult; D000477:Alkylating Agents / Q000008:administration & dosage* / Q000009:adverse effects; D003094:Collagen / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D006801:Humans; D008297:Male; D020894:Microfibrils / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D018625:Microscopy, Atomic Force; D016685:Mitomycin / Q000008:administration & dosage* / Q000009:adverse effects; D009929:Organ Size / Q000187:drug effects; D009994:Osmolar Concentration; D012590:Sclera / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure
D000477:Alkylating Agents; D016685:Mitomycin; D003094:Collagen