Characterization and validation of an isotope-dilution LC-MS/MS method for quantification of total desmosine and isodesmosine in plasma and serum.
BACKGROUND
Desmosine/isodesmosine (DES/IDS) is a promising biomarker for estimating activity of elastin degradation. RESULTS/METHODOLOGY: A stable isotope dilution LC-MS/MS method for measuring serum/plasma DES/IDS was developed and validated. The reportable range of this assay was 0.1-160 ng/ml. Serum/plasma DES/IDS level was stable at room temperature or 4°C for 20 h, and for three freeze-thaw cycles. Interferences from endogenous compounds and ion suppression/enhancing effect were also evaluated. Our results suggest the absolute necessity of using an IS in the measurement. We found that serum/plasma DES/IDS levels from patients with chronic obstructive pulmonary disease and cystic fibrosis were significantly higher compared with healthy smokers.


CONCLUSIONS
These results demonstrate that the LC-MS/MS method provides sensitive, reproducible and accurate quantification of total serum/plasma DES/IDS.
Translational Medicine Research Collaboration, TMRC Laboratory, James Arrott Drive, Dundee, DD1 9SY, UK.
Osama
O
Albarbarawi
Alun
A
Barton
Douglas
D
Miller
Charles
C
McSharry
Rekha
R
Chaudhuri
Neil C
NC
Thomson
Colin Na
CN
Palmer
Graham
G
Devereux
Jeffrey T-J
JT
Huang
D002853:Chromatography, Liquid; D003895:Desmosine / Q000097:blood*; D006801:Humans; D007201:Indicator Dilution Techniques; D007524:Isodesmosine / Q000097:blood*; D053719:Tandem Mass Spectrometry
D003895:Desmosine; D007524:Isodesmosine