Evaluating optical aberrations using fluorescent microspheres: methods, analysis, and corrective actions.
Obtaining optimal performance from a microscopy system requires careful evaluation of the entire optics train of the imaging system. This evaluation starts with a fundamental evaluation of the optical components in the microscope. Concise and visual methods are provided for understanding the optical performance of the microscope as a system using subdiffraction fluorescent microspheres to evaluate both monochromatic and polychromatic aberrations. Further practical guides are given to troubleshooting optical problems and final comments are made on optimizing sample preparation.
GE Healthcare, Issaquah, Washington, USA.
Paul C
PC
Goodwin
D000818:Animals; D002478:Cells, Cultured; D005069:Evaluation Studies as Topic; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D008856:Microscopy, Fluorescence / Q000379:methods; D008863:Microspheres
D005456:Fluorescent Dyes
Aberration; Astigmatism; Fluorescent microsphere; Optics; PSF