Synthesis of homogeneous MUC1 oligomers via a bi-directional ligation strategy.
The efficient synthesis of homogeneous MUC1 peptide oligomers using sequential ligation reactions in the N-to-C and C-to-N directions is reported. The bi-directional ligation strategy makes use of thioester formation via N → S acyl shift chemistry in combination with peptide ligation reactions and was used to prepare a library of peptide oligomers ranging in molecular mass from 3.8-9.4 kDa, comprised of between 2 and 5 repeats of the MUC1 variable number tandem repeat sequence.
School of Chemistry, The University of Sydney, Sydney, NSW 2006, Australia.
Dima
D
Al Sheikha
Brendan L
BL
Wilkinson
Gajan
G
Santhakumar
Morten
M
Thaysen-Andersen
Richard J
RJ
Payne
D008968:Molecular Conformation; D018396:Mucin-1 / Q000737:chemistry*; D010455:Peptides / Q000138:chemical synthesis* / Q000737:chemistry; D013438:Sulfhydryl Compounds / Q000737:chemistry
D018396:Mucin-1; D010455:Peptides; D013438:Sulfhydryl Compounds