A donor cornea with metastatic cells from a cutaneous malignant melanoma.
OBJECTIVE
To describe the case of a donor cornea that showed hematogenous metastatic spread of cutaneous melanoma to the sclerocorneal limbus.


METHODS
Corneal tissue obtained from a donor with cutaneous malignant melanoma was evaluated for endothelial cell density, corneal transparency, and epithelial morphology. Subsequently, hematoxylin and eosin staining and immunohistochemical characterization using S100, HMB45, Melan-A, and CD34 antibodies were performed on the corneal sections.


RESULTS
The corneal tissue was transparent with high endothelial cell density; it was graded as being suitable for transplantation according to the current eye bank criteria. However, the aggressiveness of the donor's cancer and the diffuse melanosis of the sclera led to the suspicion of malignant melanoma metastasis to the cornea. Histochemical analysis of the corneoscleral rim showed small aggregates rich in pigmented cells that were localized in cleft-like structures in the sclera, at the sclerocorneal interface and in the peripheral avascular portion of the cornea. The aggregates were positive for the melanocytic tumor markers S100, HMB45, and Melan-A; the rims of the clefts expressed the panvascular CD34 antigen, which was suggestive of neovascularization.


CONCLUSIONS
Corneal tissue from a donor with malignant cutaneous melanoma displayed neoplastic lesions of melanocytic origin that had spread from a primitive melanoma through hematogenous routes to the sclerocorneal limbus. On the basis of this finding, we believe that having a metastatic cutaneous malignant melanoma could in some cases be reviewed as an exclusionary criterion for undergoing cornea transplantation.
*Monza Eye Bank Unit, San Gerardo Hospital, Monza, Italy; †Department of Pathology, San Gerardo Hospital, Monza, Italy; and ‡Research and Development, AL.CHI.MI.A. S.r.l., Ponte San Nicolò, Italy.
Marino
M
Campanelli
Raffaela
R
Mistò
Anna
A
Limongelli
Maria G
MG
Valente
Maria S
MS
Cuttin
Jana
J
D'Amato Tóthová
D000368:Aged; D003316:Corneal Diseases / Q000473:pathology*; D016039:Corneal Transplantation; D005134:Eye Neoplasms / Q000556:secondary*; D006801:Humans; D008297:Male; D008545:Melanoma / Q000556:secondary*; D012878:Skin Neoplasms / Q000473:pathology*; D014019:Tissue Donors