Temporal analysis of oxidative effects on the pulmonary inflammatory response in mice exposed to cigarette smoke.
The most common factor related to the chronic obstructive pulmonary disease (COPD) development is the chronic smoking habit. Our study describes the temporal kinesis of pulmonary cellular influx through BALF analyses of mice acutely exposed to cigarette smoke (CS), the oxidative damage and antioxidative enzyme activities. Thirty-six mice (C57BL/6, 8weeks old, male) were divided in 6 groups: the control group (CG), exposed to ambient air, and the other 30 mice were exposed to CS. Mice exposed to CS presented, especially after the third day of exposure, different cellular subpopulations in BALF. The oxidative damage was significantly higher in CS exposed groups compared to CG. Our data showed that the evaluated inflammatory cells, observed after three days of CS exposure, indicate that this time point could be relevant to studies focusing on these cellular subpopulation activities and confirm the oxidative stress even in a short term CS exposure.
Laboratory of Metabolic Biochemistry (LBM), Department of Biological Sciences (DECBI), Center of Research in Biological Sciences (NUPEB), Federal University of Ouro Preto, Ouro Preto, MG, Brazil.
Keila Karine Duarte
KK
Campos
Rafaela Gontijo
RG
Manso
Evandro Guedes
EG
Gonçalves
Marcelo Eustáquio
ME
Silva
Wanderson Geraldo
WG
de Lima
Cristiane Alves Silva
CA
Menezes
Frank Silva
FS
Bezerra
D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry / Q000166:cytology; D002374:Catalase / Q000378:metabolism; D005434:Flow Cytometry; D005979:Glutathione Peroxidase / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018384:Oxidative Stress / Q000276:immunology*; D029424:Pulmonary Disease, Chronic Obstructive / Q000201:enzymology / Q000209:etiology* / Q000276:immunology / Q000378:metabolism; D012907:Smoking / Q000009:adverse effects / Q000276:immunology* / Q000378:metabolism; D018709:Statistics, Nonparametric; D013482:Superoxide Dismutase / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis; D013997:Time Factors
D017392:Thiobarbituric Acid Reactive Substances; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase
Cigarette smoke; Flow cytometry; Lung inflammation; Mice; Oxidative stress