Reassessment of MLST schemes for Leptospira spp. typing worldwide.
Leptospirosis is a neglected zoonosis of global importance. Several multilocus sequence typing (MLST) methods have been developed for Leptospira spp., the causative agent of leptospirosis. In this study we reassessed the most commonly used MLST schemes in a set of worldwide isolates, in order to select the loci that achieve the maximum power of discrimination for typing Leptospira spp. Global eBURST algorithm was used to detect clonal complexes among STs and phylogenetic relationships among concatenated and individual sequences were inferred through maximum likelihood (ML) analysis. The evaluation of 12 loci combined to type a subset of strains rendered 57 different STs. Seven of these loci were selected into a final scheme upon studying the number of alleles and polymorphisms, the typing efficiency, the discriminatory power and the ratio dN/dS per nucleotide site for each locus. This new 7-locus scheme was applied to a wider collection of worldwide strains. The ML tree constructed from concatenated sequences of the 7 loci identified 6 major clusters corresponding to 6 Leptospira species. Global eBURST established 8 CCs, which showed that genotypes were clearly related by geographic origin and host. ST52 and ST47, represented mostly by Argentinian isolates, grouped the higher number of isolates. These isolates were serotyped as serogroups Pomona and Icterohaemorrhagiae, showing a unidirectional correlation in which the isolates with the same ST belong to the same serogroup. In summary, this scheme combines the best loci from the most widely used MLST schemes for Leptospira spp. and supports worldwide strains classification. The Argentinian isolates exhibited congruence between allelic profile and serogroup, providing an alternative to serological methods.
Biotechnology Institute, INTA Castelar, Buenos Aires, Argentina.
Vanina
V
Varni
Biotechnology Institute, INTA Castelar, Buenos Aires, Argentina.
Paula
P
Ruybal
Molecular Epidemiology Unit, School of Health Sciences, National University of Salta, Salta, Argentina.
Juan José
JJ
Lauthier
Molecular Epidemiology Unit, School of Health Sciences, National University of Salta, Salta, Argentina.
Nicolás
N
Tomasini
Pathobiology Institute, INTA Castelar, Buenos Aires, Argentina.
Bibiana
B
Brihuega
Biogenesis Bagó, Garín, Buenos Aires, Argentina.
Ariel
A
Koval
Biotechnology Institute, INTA Castelar, Buenos Aires, Argentina. Electronic address: kcaimi@cnia.inta.gov.ar.
Karina
K
Caimi
D000818:Animals; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D006801:Humans; D007919:Leptospira / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D007922:Leptospirosis / Q000175:diagnosis* / Q000382:microbiology; D058885:Multilocus Sequence Typing / Q000379:methods*; D058069:Neglected Diseases / Q000175:diagnosis / Q000382:microbiology; D015047:Zoonoses / Q000175:diagnosis / Q000382:microbiology
D004269:DNA, Bacterial
Leptospira spp.; Multilocus sequence typing; Reassessment