Generating a non-integrating human induced pluripotent stem cell bank from urine-derived cells.
Induced pluripotent stem cell (iPS cell) holds great potential for applications in regenerative medicine, drug discovery, and disease modeling. We describe here a practical method to generate human iPS cells from urine-derived cells (UCs) under feeder-free, virus-free, serum-free condition and without oncogene c-MYC. We showed that this approach could be applied in a large population with different genetic backgrounds. UCs are easily accessible and exhibit high reprogramming efficiency, offering advantages over other cell types used for the purpose of iPS generation. Using the approach described in this study, we have generated 93 iPS cell lines from 20 donors with diverse genetic backgrounds. The non-viral iPS cell bank with these cell lines provides a valuable resource for iPS cells research, facilitating future applications of human iPS cells.
CAS Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, Guangdong, China.
Yanting
Y
Xue
Xiujuan
X
Cai
Linli
L
Wang
Baojian
B
Liao
Hui
H
Zhang
Yongli
Y
Shan
Qianyu
Q
Chen
Tiancheng
T
Zhou
Xirui
X
Li
Jundi
J
Hou
Shubin
S
Chen
Rongping
R
Luo
Dajiang
D
Qin
Duanqing
D
Pei
Guangjin
G
Pan
D000293:Adolescent; D000328:Adult; D018929:Cell Culture Techniques / Q000379:methods*; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology*; D008297:Male; D008875:Middle Aged; D014556:Urine / Q000166:cytology*; D055815:Young Adult