Sex-specific gonadal and gene expression changes throughout development in fathead minnow.
Although fathead minnows (Pimephales promelas) are commonly used as a model fish in endocrine disruption studies, past studies have not characterized sex-specific baseline expression of genes involved in sex differentiation during development in this species. Using a sex-linked DNA marker to verify gender, we evaluated the expression over time of genes involved in sex differentiation (dmrt1, cyp19a, cyp17, star, esr1, ar) in developing fathead minnows (10-45 days post hatch). Evaluation of these molecular markers in combination with gender identification help us to better understand the mechanisms regulating sex differentiation in fathead minnows and how endocrine-disrupting chemicals may alter these processes.
Department of Forestry and Natural Resources, Purdue University, West Lafayette, Ind., USA.
J K
JK
Leet
K E
KE
Lesteberg
H L
HL
Schoenfuss
A W
AW
Olmstead
J J
JJ
Amberg
G T
GT
Ankley
M S
MS
Sepúlveda
D000818:Animals; D003530:Cyprinidae / Q000235:genetics* / Q000254:growth & development*; D052244:Endocrine Disruptors / Q000494:pharmacology; D005260:Female; D015870:Gene Expression; D006066:Gonads / Q000254:growth & development*; D008297:Male; D010053:Ovary / Q000254:growth & development; D012727:Sex Characteristics; D012732:Sex Determination Analysis / Q000662:veterinary; D012733:Sex Differentiation / Q000187:drug effects / Q000235:genetics* / Q000502:physiology; D013737:Testis / Q000254:growth & development; D013997:Time Factors
D052244:Endocrine Disruptors