Vaccinia virus outperforms a panel of other poxviruses as a potent oncolytic agent for the control of head and neck squamous cell carcinoma cell lines.
BACKGROUND
Head and neck squamous cell carcinoma (HNSCC) is the fifth most common cancer worldwide. Existing therapies for advanced tumors have high failure rates and can have severe consequences in terms of pain, disfigurement, and poor speech and swallowing function. New treatment strategies are needed to improve outcomes for patients suffering with this disease and oncolytic viruses represent a promising approach.


METHODS
We infected six well-characterized HNSCC cell lines (Cal27, Detroit562, FaDu, SCC4, SCC15, SCC25), with increasing doses of a panel of poxviruses (including myxoma, vaccinia, raccoonpox and tanapox viruses) modified to express green fluorescence protein to determine which virus was the most effective oncolytic agent in cell-based assays.


RESULTS
While myxoma, raccoonpox and tanapox displayed differing efficacy in the panel of cell lines, vaccinia virus was the most potent of the tested poxviruses and was highly effective in controlling cell growth in all cell lines.


CONCLUSIONS
Oncolytic poxviruses, particularly vaccinia virus, were effective in killing HNSCC in vitro and hold promise as potential treatments for patients with HNSCC.
Department of Otolaryngology Head and Neck Surgery, Western University, London, Ont., Canada.
Anthony C
AC
Nichols
John
J
Yoo
Sung
S
Um
Neil
N
Mundi
David A
DA
Palma
Kevin
K
Fung
S Danielle
SD
Macneil
James
J
Koropatnick
Joe S
JS
Mymryk
John W
JW
Barrett
D001691:Biological Therapy / Q000379:methods; D002294:Carcinoma, Squamous Cell / Q000628:therapy; D045744:Cell Line, Tumor; D002470:Cell Survival; D006258:Head and Neck Neoplasms / Q000628:therapy; D006801:Humans; D050504:Oncolytic Viruses / Q000254:growth & development*; D011212:Poxviridae / Q000254:growth & development*