Polyamines induce adaptive responses in water deficit stressed cucumber roots.
The aim of this study was to investigate the effect of exogenous polyamines (PAs) on the membrane status and proline level in roots of water stressed cucumber (Cucumis sativus cv. Dar) seedlings. It was found that water shortage resulted in an increase of membrane injury, lipoxygenase (LOX) activity, lipid peroxidation and proline concentration in cucumber roots during progressive dehydration. PA pretreatment resulted in a distinct reduction of the injury index, and this effect was reflected by a lower stress-evoked LOX activity increase and lipid peroxide levels at the end of the stress period. In contrast, PA-supplied stressed roots displayed a higher proline accumulation. The presented results suggest that exogenous PAs are able to alleviate water deficit-induced membrane permeability and diminish LOX activity. Observed changes were accompanied by an accumulation of proline, suggesting that the accumulation of this osmolyte might be another possible mode of action for PAs to attain higher membrane stability, and in this way mitigate water deficit effects in roots of cucumber seedlings.
Department of Plant Physiology, Poznań University of Life Sciences, Wołyńska 35, 60-637, Poznań, Poland, jkubis@jay.up.poznan.pl.
Jan
J
Kubiś
Jolanta
J
Floryszak-Wieczorek
Magdalena
M
Arasimowicz-Jelonek
D002462:Cell Membrane / Q000187:drug effects; D018553:Cucumis sativus / Q000187:drug effects / Q000502:physiology*; D003681:Dehydration; D015227:Lipid Peroxidation; D008084:Lipoxygenase / Q000378:metabolism; D010940:Plant Proteins / Q000378:metabolism; D018517:Plant Roots / Q000187:drug effects / Q000502:physiology; D011073:Polyamines / Q000494:pharmacology*; D011392:Proline / Q000032:analysis / Q000378:metabolism*; D036226:Seedlings / Q000187:drug effects / Q000502:physiology; D013312:Stress, Physiological / Q000187:drug effects*; D014867:Water / Q000502:physiology*
D010940:Plant Proteins; D011073:Polyamines; D014867:Water; D011392:Proline; D008084:Lipoxygenase