Quantitative evaluation of contrast-enhanced ultrasonography in the diagnosis of chronic ischemic renal disease in a dog model.
OBJECTIVE
The aim of this feasibility study was to prospectively explore in a dog model of chronic ischemic renal disease (CIRD) the hypothesis that real-time contrast-enhanced ultrasonography (CEUS) can quantitatively evaluate the early perfusion changes of renal cortex.


METHODS
In this animal care and use committee-approved study, the model of CIRD was carried out in healthy dogs (10.0~12.0 kg, n=5), by placing the Ameroid ring constrictors on the distal portion of right renal artery through operation. CEUS monitoring of right kidney perfusion was performed by intravenous bolus injection of 0.6 ml Sulfur hexafluoride filled microbubbles (SonoVue; Bracco S.P.A., Milan, Italy) every week after operation. The slope rate of ascending curve (A) and descending curve (α), area under curve (AUC), derived peak intensity (DPI), and time to peak (TTP) were measured in renal cortex using commercial quantification software (Q-LAB version 6; Philips Medical Systems, Bothell,WA,USA). The sensitivity of CEUS was compared with blood serum urea nitrogen (BUN) and serum creatinine (SCr) level.


RESULTS
With the progression of CIRD, dogs showed delayed enhancement and perfusion in renal CEUS curve. Earliest significant changes happened 4 weeks after operation on DPI and TTP which changed from 13.04 ± 2.71 to 15.58 ± 4.75 dB and 9.03 ± 2.01 to 10.62 ± 6.04 sec, respectively (P<.05).


CONCLUSIONS
CEUS can display the perfusion changes of CIRD in the early period.
Department of Ultrasound, Zhongshan Hospital, Fudan University, Shanghai, China.
Yi
Y
Dong
Wenping
W
Wang
Jiaying
J
Cao
Peili
P
Fan
Xiyuan
X
Lin
D000818:Animals; D001774:Blood Chemical Analysis; D003287:Contrast Media; D004195:Disease Models, Animal; D004285:Dogs; D007511:Ischemia; D007672:Kidney Cortex / Q000098:blood supply; D011446:Prospective Studies; D012039:Regional Blood Flow; D051436:Renal Insufficiency, Chronic / Q000097:blood / Q000000981:diagnostic imaging* / Q000473:pathology / Q000503:physiopathology*; D014463:Ultrasonography
D003287:Contrast Media