The immunologic profile of adoptively transferred lymphocytes influences stroke outcome of recipients.
Animals that have myelin basic protein (MBP) specific lymphocytes with a Th1(+) phenotype have worse stroke outcome than those that do not. Whether these MBP specific cells contribute to worsened outcome or are merely a consequence of worse outcome is unclear. In these experiments, lymphocytes were obtained from donor animals one month after stroke and transferred to naïve recipient animals at the time of cerebral ischemia. The MBP specific phenotype of donor cells was determined prior to transfer. Animals that received either MBP specific Th1(+) or Th17(+) cells experienced worse neurological outcome, and the degree of impairment correlated with the robustness of MBP specific Th1(+) and Th17(+) responses. These data demonstrate that the immunologic phenotype of antigen specific lymphocytes influences stroke outcome.
University of Washington School of Medicine, Seattle, WA, USA.
Dannielle
D
Zierath
Juliane
J
Schulze
Allison
A
Kunze
Olga
O
Drogomiretskiy
Derek
D
Nhan
Brett
B
Jaspers
Alexander
A
Dressel
Kyra
K
Becker
D019264:Adoptive Transfer / Q000379:methods*; D000818:Animals; D002478:Cells, Cultured; D016130:Immunophenotyping; D016131:Lymphocyte Subsets / Q000276:immunology* / Q000378:metabolism* / Q000637:transplantation; D017710:Lymphocyte Transfusion / Q000379:methods*; D008297:Male; D051381:Rats; D011917:Rats, Inbred Lew; D013154:Spleen / Q000276:immunology; D020521:Stroke / Q000276:immunology* / Q000601:surgery*; D016896:Treatment Outcome
Immune response; Lymphocyte; Stroke; Th1; Th17