Antioxidant, antibrowning, and cytoprotective activities of Ligustrum robustum (Rxob.) Blume extract.
The antioxidant, antibrowning, and cytoprotective activities of Ligustrum robustum (Rxob.) Blume extract (LRE) were investigated and the main antioxidant component was isolated and identified. With its high content of phenols and flavonoids, the LRE showed remarkable antioxidant capacity to scavenge free radicals in vitro and to inhibit oil oxidation in a peanut oil system. Moreover, LRE was observed to inhibit tyrosinase action and browning of fresh-cut apple slices effectively. Furthermore, the cytoprotective activity of LRE was evaluated in a human intestine model using Caco-2 cell lines. According to the activity-guided isolation and identification, by using column chromatography, high-performance liquid chromatography, time-of-flight mass spectrometry, and nuclear magnetic resonance analyses, ursolic acid was characterized as the main antioxidant component of LRE; it showed the strongest free radical-scavenging activity. The results suggested that L. robustum (Rxob.) Blume could be a new resource for preparing functional food and nutraceutical products for use in food and pharmacology industries.
Dept. of Food Engineering, Sichuan Univ. Chengdu, 610065, PR China.
Zhi-Long
ZL
Yu
Wei-Cai
WC
Zeng
D000975:Antioxidants / Q000737:chemistry*; D018938:Caco-2 Cells; D002851:Chromatography, High Pressure Liquid; D019610:Cytoprotection; D005419:Flavonoids / Q000032:analysis / Q000737:chemistry; D005524:Food Technology; D005638:Fruit / Q000737:chemistry; D006801:Humans; D006861:Hydrogen Peroxide; D031659:Ligustrum / Q000737:chemistry*; D050356:Lipid Metabolism; D009682:Magnetic Resonance Spectroscopy; D015416:Maillard Reaction; D027845:Malus / Q000737:chemistry; D013058:Mass Spectrometry; D014442:Monophenol Monooxygenase / Q000037:antagonists & inhibitors / Q000378:metabolism; D010636:Phenols / Q000032:analysis / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry*; D014315:Triterpenes / Q000737:chemistry; D000097245:Ursolic Acid
D000975:Antioxidants; D005419:Flavonoids; D006861:Hydrogen Peroxide; D014442:Monophenol Monooxygenase; D010636:Phenols; D010936:Plant Extracts; D014315:Triterpenes; D000097245:Ursolic Acid
Ligustrum robustum (Rxob.) Blume; antibrowning activity; antioxidant activity; cytoprotective activity; ursolic acid