Microbial community shifts and biogas conversion computation during steady, inhibited and recovered stages of thermophilic methane fermentation on chicken manure with a wide variation of ammonia.
The thermophilic methane fermentation of chicken manure (10% TS) was investigated within a wide range of ammonia. Microbiological analysis showed significant shifts in Archaeal and Bacterial proportions with VFA accmulation and CH4 formation before and after inhibition. VFA accumulated sharply with lower methane production, 0.29 L/g VS, than during the steady stage, 0.32 L/g VS. Biogas production almost ceased with the synergy inhibition of TAN (8000 mg/L) and VFA (25,000 mg/L). Hydrogenotrophic Methanothermobacter thermautotrophicus str. was the dominate archaea with 95% in the inhibition stage and 100% after 40 days recovery compared to 9.3% in the steady stage. Aceticlastic Methanosarcina was not encountered with coincided phenomenal of high VFA in the inhibition stage as well as recovery stage. Evaluation of the microbial diversity and functional bacteria indicated the dominate phylum of Firmicutes were 94.74% and 84.4% with and without inhibition. The microbial community shifted significantly with elevated ammonia concentration affecting the performance.
Graduate School of Environmental Studies, Tohoku University, 6-6-06 Aza-Aoba, Aramaki, Aoba-ku, Sendai, Miyagi 980-8579, Japan.
Qigui
Q
Niu
Graduate School of Environmental Studies, Tohoku University, 6-6-06 Aza-Aoba, Aramaki, Aoba-ku, Sendai, Miyagi 980-8579, Japan; College of Chemical Engineering, China University of Petroleum, Beijing 102249, China.
Wei
W
Qiao
College of Resources and Environment Northwest A&F University, Yangling 712100, China.
Hong
H
Qiang
Graduate School of Environmental Studies, Tohoku University, 6-6-06 Aza-Aoba, Aramaki, Aoba-ku, Sendai, Miyagi 980-8579, Japan; Department of Civil and Environmental Engineering, Graduate School of Engineering Tohoku University, 6-6-06 Aza-Aoba, Aramaki, Aoba-ku, Sendai, Miyagi 980-8579, Japan; Key Lab of Northwest Water Resource, Environment and Ecology, MOE, Xi'an University of Architecture and Technology, Xi'an, 710055, PR China. Electronic address: yyli@epli.civil.tohoku.ac.jp.
Yu-You
YY
Li
D000383:Agriculture; D000641:Ammonia / Q000737:chemistry*; D000818:Animals; D001105:Archaea; D056804:Biofuels; D019149:Bioreactors / Q000382:microbiology*; D002645:Chickens; D005285:Fermentation; D006868:Hydrolysis; D008372:Manure; D008697:Methane / Q000737:chemistry*; D017013:Methanobacteriaceae / Q000378:metabolism; D010802:Phylogeny; D025341:Principal Component Analysis; D012037:Refuse Disposal / Q000379:methods; D013696:Temperature
D056804:Biofuels; D008372:Manure; D000641:Ammonia; D008697:Methane
Ammonia inhibition; Chicken manure; Methane fermentation; Microbial community shift; Thermophilic