Metagenomic analysis of fungal communities inhabiting the fairy ring zone of Tricholoma matsutake.
Tricholoma matsutake, an ectomycorrhiza that has mutual relationships with the rootlet of Pinus denisflora, forms a fruiting body that serves as a valuable food in Asia. However, the artificial culture of this fungus has not been successful. Soil fungi, including T. matsutake, coexist with many other microorganisms and plants; therefore, complex microbial communities have an influence on the fruiting body formation of T. matsutake. Here, we report on the structures of fungal communities associated with the fairy ring of T. matsutake through the pyrosequencing method. Soil samples were collected inside the fairy ring zone, in the fairy ring zone, and outside the fairy ring zone. A total of 37,125 sequencing reads were obtained and 728 to 1,962 operational taxonomic units (OTUs) were observed in the sampling zones. The fairy ring zone had the lowest OTUs and the lowest fungal diversity of all sampling zones. The number of OTUs and fungal taxa inside and outside the fairy ring zone was, respectively, about 2 times and 1.5 times higher than the fairy ring. Taxonomic analysis showed that each sampling zone has different fungal communities. In particular, out of 209 genera total, 6 genera in the fairy ring zone, such as Hemimycena, were uniquely present and 31 genera, such as Mycena, Boletopsis, and Repetophragma, were specifically absent. The results of metagenomic analysis based on the pyrosequencing indicate a decrease of fungal communities in the fairy ring zone and changes of fungal communities depending on the fairy ring growth of T. matsutake.
Department of Life Sciences and Biotechnology, Kyungpook National University, Daegu 702-701, Republic of Korea.
Miae
M
Kim
Hyeokjun
H
Yoon
Young-Hyun
YH
You
Ye-Eun
YE
Kim
Ju-Ri
JR
Woo
Yeonggyo
Y
Seo
Gyeong-Min
GM
Lee
Young Ja
YJ
Kim
Won-Sik
WS
Kong
Jong-Guk
JG
Kim
D001208:Asia; D058448:Biota; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D056186:Metagenomics; D008969:Molecular Sequence Data; D038821:Mycorrhizae / Q000145:classification* / Q000235:genetics*; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D055439:Tricholoma / Q000145:classification* / Q000235:genetics*
D004271:DNA, Fungal