Involvement of miR-20a in promoting gastric cancer progression by targeting early growth response 2 (EGR2).
Gastric cancer (GC) is one of the most common cancers, with high incidences in East Asia. microRNAs (miRNAs) play essential roles in the carcinogenesis of GC. miR-20a was elevated in GC, while the potential function of miR-20a was poorly understood. miR-20a expression was examined in GC tissues and cell lines. The effects of miR-20a on the growth, migration, invasion, and chemoresistance of GC cells were examined. Luciferase reporter assay and Western blot were used to screen the target of miR-20a. miR-20a was increased in GC tissues and cell lines. miR-20a promoted the growth, migration and invasion of GC cells, enhanced the chemoresistance of GC cells to cisplatin and docetaxel. Luciferase activity and Western blot confirmed that miR-20a negatively regulated EGR2 expression. Overexpression of EGR2 significantly attenuated the oncogenic effect of miR-20a. miR-20a was involved in the carcinogenesis of GC through modulation of the EGR2 signaling pathway.
Department of General Surgery, Xinqiao Hospital, Third Military Medical University, Xinqiao Road, Chongqing 400037, China. xiangsonlee@163.com
Xiangsheng
X
Li
Zhichao
Z
Zhang
Ming
M
Yu
Liqi
L
Li
Guangsheng
G
Du
Weidong
W
Xiao
Hua
H
Yang
D000970:Antineoplastic Agents / Q000494:pharmacology; D063646:Carcinogenesis / Q000235:genetics*; D059447:Cell Cycle Checkpoints / Q000235:genetics; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation; D002945:Cisplatin / Q000494:pharmacology; D000077143:Docetaxel; D019008:Drug Resistance, Neoplasm / Q000235:genetics; D051767:Early Growth Response Protein 2 / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D005260:Female; D015870:Gene Expression; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D009361:Neoplasm Invasiveness / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis; D013274:Stomach Neoplasms / Q000235:genetics* / Q000378:metabolism; D043823:Taxoids / Q000494:pharmacology
D000970:Antineoplastic Agents; C497326:EGR2 protein, human; D051767:Early Growth Response Protein 2; C534451:MIRN20a microRNA, human; D035683:MicroRNAs; D012333:RNA, Messenger; D043823:Taxoids; D000077143:Docetaxel; D002945:Cisplatin