Inflammatory effect of monopolar radiofrequency treatment on collagen fibrils in rabbit skins.
This study quantitatively examined the effects of monopolar radiofrequency (RF) treatment on the progressive nanostructural changes in the inflammatory effect of in vivo rabbit dermal collagen fibrils during postoperative 7 days. Conventional histologic analysis and atomic force microscopy (AFM) were employed to assess the nanostructural dermal response in 6 RF groups that underwent 2 passes of RF treatments (10 and 20 W), and 1 untreated control rabbit. After monopolar RF treatment, the rabbit skin clearly showed changes in the collagen network structure. The RF-treated group showed regular parallel arrangement of collagen fibrils whereas normal group showed tangled nanostructures. This phenomenon was remarkable at postoperative 7 days. Therefore, monopolar RF treatment leads to underlying collagen contracture and promotes new collagen formation. A multi-pass treatment of low-energy RF led to the highest contraction of collagen fibrils at the nanostructural level, compared to a single pass of high-energy RF.
Department of Biomedical Engineering and Healthcare Industry Research Institute, College of Medicine, Kyung Hee University, Seoul 130-701, Republic of Korea.
Samjin
S
Choi
Youjin
Y
Cheong
Jae-Ho
JH
Shin
Kyung-Hyun
KH
Jin
Hun-Kuk
HK
Park
D000818:Animals; D001706:Biopsy; D003094:Collagen / Q000378:metabolism / Q000528:radiation effects*; D020405:Dermis / Q000378:metabolism / Q000473:pathology / Q000528:radiation effects; D007249:Inflammation / Q000209:etiology* / Q000378:metabolism / Q000473:pathology; D018836:Inflammation Mediators / Q000378:metabolism; D008297:Male; D020894:Microfibrils / Q000378:metabolism / Q000528:radiation effects*; D018625:Microscopy, Atomic Force; D061208:Pulsed Radiofrequency Treatment / Q000009:adverse effects; D011817:Rabbits; D011846:Radio Waves / Q000009:adverse effects*; D012867:Skin / Q000378:metabolism / Q000473:pathology / Q000528:radiation effects* / Q000648:ultrastructure
D018836:Inflammation Mediators; D003094:Collagen