Light-mediated in cell downregulation of G-quadruplex-containing genes using a photo-caged ligand.
The use of a caged G-quadruplex ligand allows for transcriptional control of quadruplex-containing genes using UV light as an external trigger. An important oncogene, SRC, involved in the initiation and proliferation of epithelial tumours is shown to be significantly downregulated in cells treated by the caged ligand in synergy with UV light treatment.
Department of Chemistry, The University of Cambridge, Lensfield Road, Cambridge, CB2 1EW, UK. sb10031@cam.ac.uk.
Pierre
P
Murat
Michael V
MV
Gormally
Debbie
D
Sanders
Marco
M
Di Antonio
Shankar
S
Balasubramanian
D000634:Aminoquinolines / Q000737:chemistry / Q000494:pharmacology*; D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D015536:Down-Regulation / Q000187:drug effects*; D004354:Drug Screening Assays, Antitumor; D054856:G-Quadruplexes / Q000187:drug effects*; D016391:Genes, src / Q000187:drug effects* / Q000235:genetics; D006801:Humans; D008024:Ligands; D015394:Molecular Structure; D009375:Neoplasms, Glandular and Epithelial / Q000188:drug therapy* / Q000235:genetics / Q000473:pathology; D055668:Photochemical Processes; D010848:Picolinic Acids / Q000737:chemistry / Q000494:pharmacology*; D013329:Structure-Activity Relationship; D014466:Ultraviolet Rays
D000634:Aminoquinolines; D000970:Antineoplastic Agents; D008024:Ligands; D010848:Picolinic Acids; C567962:pyridostatin