Oligoclonal expansions of mucosal T cells in Crohn's disease predominate in NKG2D-expressing CD4 T cells.
Crohn's disease (CD) is an inflammatory pathology of the mucosal intestine that results from uncontrolled immune response towards commensal microbes. Clonal expansions of T cells have been found in patients with CD suggesting an antigen-specific stimulation of pathogenic T cells. Here we show, using T-cell receptor repertoire analysis by real-time PCR, that oligoclonal expansions are found in both CD8+ and CD4+ T cells in the blood and intestinal mucosa of CD patients. The majority of CD4+ T-cell-expanded clones are CD4+NKG2D+ T cells. These clonal expansions were found in both inflamed and neighboring healthy tissue and were persisting during the course of the disease. The presence of these CD4+NKG2D+ T-cell clones at the macroscopically normal edge of the surgical resection might be predictive of inflammation relapse post surgery.
1] AVENIR INSERM, Paris, France [2] INSERM U940, Institut Universitaire d'Hématologie Hôpital Saint-Louis, Paris, France.
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Camus
Immunobiology Center, Mount Sinai School of Medicine, New York, New York, USA.
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Esses
1] AVENIR INSERM, Paris, France [2] Gastroenterology Department, AP-HP, Hôpital Saint-Louis, Paris, France.
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Pariente
1] AVENIR INSERM, Paris, France [2] INSERM U940, Institut Universitaire d'Hématologie Hôpital Saint-Louis, Paris, France.
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Le Bourhis
1] INSERM U940, Institut Universitaire d'Hématologie Hôpital Saint-Louis, Paris, France [2] Université Paris Diderot, Paris, France.
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Douay
1] AVENIR INSERM, Paris, France [2] INSERM U940, Institut Universitaire d'Hématologie Hôpital Saint-Louis, Paris, France.
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Chardiny
1] AVENIR INSERM, Paris, France [2] INSERM U940, Institut Universitaire d'Hématologie Hôpital Saint-Louis, Paris, France.
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Mocan
1] INSERM U940, Institut Universitaire d'Hématologie Hôpital Saint-Louis, Paris, France [2] Université Paris Diderot, Paris, France.
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Benlagha
1] INSERM U940, Institut Universitaire d'Hématologie Hôpital Saint-Louis, Paris, France [2] Université Paris Diderot, Paris, France.
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Clave
1] INSERM U940, Institut Universitaire d'Hématologie Hôpital Saint-Louis, Paris, France [2] Université Paris Diderot, Paris, France.
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Toubert
Immunobiology Center, Mount Sinai School of Medicine, New York, New York, USA.
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Mayer
1] AVENIR INSERM, Paris, France [2] INSERM U940, Institut Universitaire d'Hématologie Hôpital Saint-Louis, Paris, France [3] Gastroenterology Department, AP-HP, Hôpital Saint-Louis, Paris, France [4] Université Paris Diderot, Paris, France.
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Allez
D000328:Adult; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology* / Q000378:metabolism*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D016022:Case-Control Studies; D003424:Crohn Disease / Q000276:immunology* / Q000378:metabolism* / Q000601:surgery; D005260:Female; D006801:Humans; D007082:Ileum / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D007413:Intestinal Mucosa / Q000276:immunology* / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D055655:NK Cell Lectin-Like Receptor Subfamily K / Q000378:metabolism*; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000378:metabolism; D012008:Recurrence; D016176:T-Lymphocyte Subsets / Q000276:immunology / Q000378:metabolism; D055815:Young Adult
D055655:NK Cell Lectin-Like Receptor Subfamily K; D016693:Receptors, Antigen, T-Cell, alpha-beta