Diagnostic assays for identification of microorganisms and antimicrobial resistance determinants directly from positive blood culture broth.
The detection of blood stream infections is one of the most important functions of the clinical microbiology laboratory. Sepsis is a clinical emergency, and mortality increases if commencement of appropriate antimicrobial therapy is delayed. Automated blood culture systems are the most sensitive approach for detection of the causative agent of sepsis. Several laboratory methods have been developed to expedite identification of organisms directly from positive blood culture broth. The principle and analytical performance characteristics of these methods are described in this review.
Division of Laboratory and Genomic Medicine, Department of Pathology and Immunology, Washington University School of Medicine, Washington University in St Louis, 660 South Euclid Avenue, Campus Box 8118, St Louis, MO 63110, USA.
Morgan A
MA
Pence
Erin
E
McElvania TeKippe
Carey-Ann D
CA
Burnham
D001331:Automation / Q000295:instrumentation; D001769:Blood / Q000382:microbiology*; D004352:Drug Resistance, Microbial; D004797:Enzyme-Linked Immunosorbent Assay; D006094:Gram-Positive Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D055624:Methicillin-Resistant Staphylococcus aureus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D008828:Microbiological Techniques; D016133:Polymerase Chain Reaction / Q000295:instrumentation / Q000379:methods; D021241:Spectrometry, Mass, Electrospray Ionization; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013203:Staphylococcal Infections / Q000175:diagnosis / Q000382:microbiology
Bacteremia; Blood culture; MRSA; Molecular diagnostics; Rapid pathogen detection; Staphylococcus aureus