Role of the Arabidopsis PIN6 auxin transporter in auxin homeostasis and auxin-mediated development.
Plant-specific PIN-formed (PIN) efflux transporters for the plant hormone auxin are required for tissue-specific directional auxin transport and cellular auxin homeostasis. The Arabidopsis PIN protein family has been shown to play important roles in developmental processes such as embryogenesis, organogenesis, vascular tissue differentiation, root meristem patterning and tropic growth. Here we analyzed roles of the less characterised Arabidopsis PIN6 auxin transporter. PIN6 is auxin-inducible and is expressed during multiple auxin-regulated developmental processes. Loss of pin6 function interfered with primary root growth and lateral root development. Misexpression of PIN6 affected auxin transport and interfered with auxin homeostasis in other growth processes such as shoot apical dominance, lateral root primordia development, adventitious root formation, root hair outgrowth and root waving. These changes in auxin-regulated growth correlated with a reduction in total auxin transport as well as with an altered activity of DR5-GUS auxin response reporter. Overall, the data indicate that PIN6 regulates auxin homeostasis during plant development.
Australian Research Council Centre of Excellence in Plant Energy Biology, College of Medicine, Biology and Environment, Research School of Biology, The Australian National University, Canberra, Australia. christopher.cazzonelli@anu.edu.au
Christopher I
CI
Cazzonelli
Marleen
M
Vanstraelen
Sibu
S
Simon
Kuide
K
Yin
Ashley
A
Carron-Arthur
Nazia
N
Nisar
Gauri
G
Tarle
Abby J
AJ
Cuttriss
Iain R
IR
Searle
Eva
E
Benkova
Ulrike
U
Mathesius
Josette
J
Masle
Jiří
J
Friml
Barry J
BJ
Pogson
D017360:Arabidopsis / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant / Q000235:genetics / Q000502:physiology; D007210:Indoleacetic Acids / Q000378:metabolism*; D026901:Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism*; D018517:Plant Roots / Q000235:genetics / Q000254:growth & development / Q000378:metabolism
D029681:Arabidopsis Proteins; D007210:Indoleacetic Acids; D026901:Membrane Transport Proteins; C581623:PIN6 protein, Arabidopsis