Chinese goose (Anser cygnoides) CD8a: cloning, tissue distribution and immunobiological in splenic mononuclear cells.
CD8 molecule is a cell membrane glycoprotein, which plays an important role in cell-mediated immunity. Here, we identified Chinese goose CD8α (goCD8α) gene for the first time. The full-length cDNA of goCD8α is 1459bp in length and contains a 711bp open reading frame. Phylogenetic analysis shows that the waterfowl CD8α formed a monophyletic group. Semi-quantitative RT-PCR analysis showed that transcripts of goCD8α mRNA were high in the immune-related organs and mucosal immune system in gosling, and high in thymus and spleen comparing to other immune-related tissues in goose. The obvious increase of CD8α expression was observed in spleen of acute new type gosling viral enteritis virus (NGVEV) infected bird, while the increase of CD8α were observed in the thymus, bursa of fabricius, and cecum of chronic infected bird. The CD8α mRNA transcription level in spleen mononuclear cells was significantly up-regulated when stimulated by phytohemagglutinin, but not by lipopolysaccharide in vitro.
Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan 611130, PR China; Avian Disease Research Center, College of Veterinary Medicine of Sichuan Agricultural University, 46 Xinkang Road, Ya'an, Sichuan 625014, PR China.
Qiurong
Q
Zhao
Fei
F
Liu
Shun
S
Chen
Xiaoling
X
Yan
Yulin
Y
Qi
Mingshu
M
Wang
Renyong
R
Jia
Dekang
D
Zhu
Mafeng
M
Liu
Xiaoyue
X
Chen
Anchun
A
Cheng
D000595:Amino Acid Sequence; D000818:Animals; D030161:Avian Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D016827:CD8 Antigens / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D002432:Cecum / Q000378:metabolism; D003001:Cloning, Molecular; D005777:Geese / Q000235:genetics* / Q000276:immunology / Q000378:metabolism / Q000821:virology; D007963:Leukocytes, Mononuclear / Q000276:immunology*; D008070:Lipopolysaccharides / Q000276:immunology; D008969:Molecular Sequence Data; D009928:Organ Specificity; D010321:Parvoviridae / Q000276:immunology; D010835:Phytohemagglutinins / Q000276:immunology; D012333:RNA, Messenger / Q000378:metabolism; D013154:Spleen / Q000276:immunology / Q000378:metabolism; D013950:Thymus Gland / Q000378:metabolism; D014018:Tissue Distribution; D014158:Transcription, Genetic
D030161:Avian Proteins; D016827:CD8 Antigens; D008070:Lipopolysaccharides; D010835:Phytohemagglutinins; D012333:RNA, Messenger
AAP; AP; AUAP; Adapter Primer; BF; BLAST; Basic Local Alignment Search Tool; CD8α; CHCD8α; CIS; CMC; CMI; CT; CTLs; GSP; Goose; HG; IEL; Immunobiological activity; LD(50); LPS; MHC; MIS; MNCs; Molecular cloning; NGVEV; NGVEV infection; OFR; PHA; PI; RACE; RT-PCR; T cell antigen receptor; TCID50; TCR; Tissue distribution; TuCD8α; UTR; abridged anchor primer; abridged universal amplification primer; bursa of fabricius; cDNA; cecal tonsil; cell-mediated cytotoxicity; cell-mediated immune; chicken CD8α; complementary to RNA; cutaneous immune system; cytotoxic T lymphocytes; gene specific primer; goCD8α; goose CD8α; harderian gland; intestinal intraepithelial lymphocytes; lethal dose; lipopolysaccharide; major histocompatibility complex; monocolor cells; mucosal immune system; new type gosling viral enteritis virus; open reading frame; phytohemagglutinin; post infection; qPCR; quantitative PCR; rapid amplification of cDNA ends; reverse transcription-polymerase chain reaction; tissue culture infective dose; turkey CD8α; untranslated region