Automethylation of protein arginine methyltransferase 8 (PRMT8) regulates activity by impeding S-adenosylmethionine sensitivity.
Protein arginine methyltransferase (PRMT) 8 is unique among the PRMTs, as it has a highly restricted tissue expression pattern and an N terminus that contains two automethylation sites and a myristoylation site. PRMTs catalyze the transfer of a methyl group from S-adenosylmethionine (AdoMet) to a peptidylarginine on a protein substrate. Currently, the physiological roles, regulation, and cellular substrates of PRMT8 are poorly understood. However, a thorough understanding of PRMT8 kinetics should provide insights into each of these areas, thereby enhancing our understanding of this unique enzyme. In this study, we determined how automethylation regulates the enzymatic activity of PRMT8. We found that preventing automethylation with lysine mutations (preserving the positive charge of the residue) increased the turnover rate and decreased the Km of AdoMet but did not affect the Km of the protein substrate. In contrast, mimicking automethylation with phenylalanine (i.e. mimicking the increased hydrophobicity) decreased the turnover rate. The inhibitory effect of the PRMT8 N terminus could be transferred to PRMT1 by creating a chimeric protein containing the N terminus of PRMT8 fused to PRMT1. Thus, automethylation of the N terminus likely regulates PRMT8 activity by decreasing the affinity of the enzyme for AdoMet.
From the Department of Chemical Physiology, The Scripps Research Institute, La Jolla, California 92037 and.
Myles B C
MB
Dillon
Heather L
HL
Rust
Paul R
PR
Thompson
Kerri A
KA
Mowen
D002384:Catalysis; D006367:HeLa Cells; D006801:Humans; D008239:Lysine / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism*; D008745:Methylation; D016297:Mutagenesis, Site-Directed; D009154:Mutation; D010649:Phenylalanine / Q000378:metabolism; D011485:Protein Binding; D011499:Protein Processing, Post-Translational; D017434:Protein Structure, Tertiary; D011484:Protein-Arginine N-Methyltransferases / Q000378:metabolism*; D012436:S-Adenosylmethionine / Q000378:metabolism*
D008565:Membrane Proteins; D010649:Phenylalanine; D012436:S-Adenosylmethionine; C505447:PRMT8 protein, human; D011484:Protein-Arginine N-Methyltransferases; D008239:Lysine
Enzyme Catalysis; Enzyme Inactivation; Enzyme Kinetics; Histone Methylation; Protein Methylation; S-Adenosylmethionine (SAM)