Method development and validation for determining 1,3-butadiene in human blood by gas chromatography-mass spectrometry and head-space gas chromatography.
To develop a simple, validated method for identifying and quantifying 1,3-butadiene (BD) in human blood by gas chromatography-mass spectrometry (GC-MS) and head-space gas chromatography (HS-GC). BD was identified by GC-MS and HS-GC, and quantified by HS-GC. The method showed that BD had a good linearity from 50 to 500 microg/mL (r > 0.99). The limits of detection and quantification were 10 microg/mL and 50 microg/mL, respectively. Both the intra-day precision and inter-day precision were < 6.08%, and the accuracy was 96.98%-103.81%. The method was applied to an actual case, and the concentration of BD in the case was 242 microg/mL in human blood. This simple method is found to be useful for the routine forensic analysis of acute exposure to BD.
College of Pharmaceutical Science, Soochow University, Suzhou 215123, China. ivy-yuonly@163.com
Su-Jing
SJ
Zhang
Bao-Hua
BH
Shen
Xian-Yi
XY
Zhuo
D000328:Adult; D002070:Butadienes / Q000097:blood* / Q000506:poisoning*; D053593:Forensic Toxicology / Q000379:methods; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D005739:Gas Poisoning; D006801:Humans; D008297:Male; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012997:Solvents / Q000737:chemistry; D013696:Temperature
D002070:Butadienes; D012997:Solvents; C031763:1,3-butadiene