Impact of Bacillus Calmette-Guérin Moreau vaccine on lung remodeling in experimental asthma.
We analyzed the effects of different administration routes and application times of the BCG-Moreau strain on airway and lung inflammation and remodeling in a murine model of allergic asthma. BALB/c mice (n=168) were divided into two groups. The first group received BCG-Moreau strain while the second group received saline using the same protocol. BCG or saline were intradermally or intranasally injected one or two months before the induction of asthma. Mice were further sensitized and challenged with ovalbumin or received saline. Twenty-four hours after the last challenge, BCG prevented the triggering of pro-inflammatory cytokines, probably by increasing Foxp3 and interleukin (IL)-10, modulating eosinophil infiltration and collagen fiber deposition, thus reducing airway hyperresponsiveness. In conclusion, BCG-Moreau prevented lung remodeling in the present model of allergic asthma, regardless of administration route and time of vaccination. These beneficial effects may be related to the increase in regulatory T cells and to IL-10 production in tandem with decreased Th2 cytokines (IL-4, IL-5, and IL-13).
Laboratory of Pulmonary Investigation, Carlos Chagas Filho Biophysics Institute, Federal University of Rio de Janeiro, Av. Carlos Chagas Filho, 373, Bloco G, Sala G1-019, Ilha do Fundão, 21941-902 Rio de Janeiro, RJ, Brazil.
Cynthia dos Santos
Cdos S
Samary
Mariana Alves
MA
Antunes
Johnatas Dutra
JD
Silva
Adriana Lopes da
AL
Silva
Carla Cristina de
CC
Araújo
Ilka
I
Bakker-Abreu
Bruno Lourenço
BL
Diaz
Sandra
S
Fernezlian
Edwin Roger
ER
Parra
Vera Luiza
VL
Capelozzi
Pedro Leme
PL
Silva
José Roberto
JR
Lapa e Silva
Patricia Rieken Macedo
PR
Rocco
D056151:Airway Remodeling / Q000187:drug effects* / Q000276:immunology; D000818:Animals; D000831:Animals, Newborn; D001249:Asthma / Q000276:immunology / Q000473:pathology / Q000517:prevention & control; D001500:BCG Vaccine / Q000494:pharmacology / Q000627:therapeutic use*; D001992:Bronchoalveolar Lavage Fluid; D016084:Bronchoconstriction / Q000187:drug effects; D004195:Disease Models, Animal; D051858:Forkhead Transcription Factors / Q000235:genetics / Q000378:metabolism; D005786:Gene Expression Regulation; D008168:Lung / Q000187:drug effects* / Q000473:pathology / Q000503:physiopathology / Q000648:ultrastructure; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D046529:Microscopy, Electron, Transmission; D011175:Positive-Pressure Respiration; D012132:Respiratory Muscles / Q000473:pathology / Q000648:ultrastructure
D001500:BCG Vaccine; D051858:Forkhead Transcription Factors; C498833:Foxp3 protein, mouse
Airway remodeling; Airway resistance; Asthma; BCG vaccine; Eosinophilia