Genome-wide identification and expression analysis of MAPK and MAPKK gene family in Malus domestica.
MAPK signal transduction modules play crucial roles in regulating many biological processes in plants, which are composed of three classes of hierarchically organized protein kinases, namely MAPKKKs, MAPKKs, and MAPKs. Although genome-wide analysis of this family has been carried out in some species, little is known about MAPK and MAPKK genes in apple (Malus domestica). In this study, a total of 26 putative apple MAPK genes (MdMPKs) and 9 putative apple MAPKK genes (MdMKKs) have been identified and located within the apple genome. Phylogenetic analysis revealed that MdMAPKs and MdMAPKKs could be divided into 4 subfamilies (groups A, B, C and D), respectively. The predicted MdMAPKs and MdMAPKKs were distributed across 13 out of 17 chromosomes with different densities. In addition, analysis of exon-intron junctions and of intron phase inside the predicted coding region of each candidate gene has revealed high levels of conservation within and between phylogenetic groups. According to the microarray and expressed sequence tag (EST) analysis, the different expression patterns indicate that they may play different roles during fruit development and rootstock-scion interaction process. Moreover, MAPK and MAPKK genes were performed expression profile analyses in different tissues (root, stem, leaf, flower and fruit), and all of the selected genes were expressed in at least one of the tissues tested, indicating that the MAPKs and MAPKKs are involved in various aspects of physiological and developmental processes of apple. To our knowledge, this is the first report of a genome-wide analysis of the apple MAPK and MAPKK gene family. This study provides valuable information for understanding the classification and putative functions of the MAPK signal in apple.
National Research Center for Apple Engineering and Technology, College of Horticulture Science and Technology, State Key Laboratory of Crop Biology, Shandong Agricultural University, Taian, Shandong 271018, PR China.
Shizhong
S
Zhang
Ruirui
R
Xu
Xiaocui
X
Luo
Zesheng
Z
Jiang
Huairui
H
Shu
D002874:Chromosome Mapping; D032461:Chromosomes, Plant; D005091:Exons / Q000235:genetics; D048049:Extracellular Signal-Regulated MAP Kinases / Q000235:genetics*; D020869:Gene Expression Profiling; D015971:Gene Expression Regulation, Enzymologic; D018506:Gene Expression Regulation, Plant; D055106:Genome-Wide Association Study; D007438:Introns / Q000235:genetics; D027845:Malus / Q000201:enzymology / Q000235:genetics*; D046228:Microarray Analysis; D020929:Mitogen-Activated Protein Kinase Kinases / Q000235:genetics*; D005810:Multigene Family / Q000235:genetics; D010802:Phylogeny
D048049:Extracellular Signal-Regulated MAP Kinases; D020929:Mitogen-Activated Protein Kinase Kinases
Apple GFDB database; Apple Gene Function and Gene Family Database; BLAST; Basic Local Alignment Search Tool; GDR; GSDS; GWD; Gene Structure Display Server; Gene family; Genome Database for Rosaceae; Genome-Wide Duplication; HMM; Hidden Markov Model; MAPK; MAPK kinase; MAPKK; MEGA; MUSCLE; MW; Malus domestica; Molecular Evolutionary Genetics Analysis; Multiple Sequence Comparison by Log-Expectation; NCBI; NJ; National Center for Biotechnology Information; ORF; Pfam; Protein family; SMART; Simple Modular Architecture Research Tool; aa; amino acid; isoelectric points; mitogen-activated protein kinase; molecular weight; neighbor-joining; open reading frame; pIs