New bioactive fungal molecules with high antioxidant and antimicrobial capacity isolated from Cerrena unicolor idiophasic cultures.
Three bioactive fractions, extracellular laccase (ex-LAC), crude endopolysaccharides (c-EPL), and a low molecular subfraction of secondary metabolites (ex-LMS), were isolated from the idiophasic cultures of the white rot fungus Cerrena unicolor. For the first time, we determined the antioxidant properties of these samples by chemiluminometric measurement (a) and assessment of the scavenging effect on ABTS (b) and the DPPH reduction rate (c). The highest reducing capability was found for the ex-LMS fraction: 39-90% for (a), 20-90% for (b), and 10-59% for (c) at the concentration of 6.25-800 µg/mL. The scavenging abilities of the C. unicolor c-EPL were between 36 and 70% for (a), 2 and 60% for (b), and 28 and 32% for (c) at the concentration of 6.25-800 µg/mL. A very high prooxidative potential was observed for the ex-LAC probes. The preliminary toxicity tests were done using the Microtox system and revealed the following percentage of the toxic effect against Vibrio fischeri: 85.37% for c-EPL, 50.67% for ex-LAC, and 99.8% for ex-LMS, respectively. The ex-LAC sample showed the antibacterial activity against Escherichia coli, c-EPL against Staphylococcus aureus, and ex-LMS against both bacterial strains, respectively, but the stronger inhibitory effect was exerted on S. aureus.
Department of Biochemistry, Maria Curie-Skłodowska University, Lublin, Poland. magdalena.jaszek@poczta.umcs.lublin.pl
Magdalena
M
Jaszek
Monika
M
Osińska-Jaroszuk
Grzegorz
G
Janusz
Anna
A
Matuszewska
Dawid
D
Stefaniuk
Justyna
J
Sulej
Jolanta
J
Polak
Marta
M
Ruminowicz
Krzysztof
K
Grzywnowicz
Anna
A
Jarosz-Wilkołazka
D000890:Anti-Infective Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000378:metabolism* / Q000494:pharmacology; D004926:Escherichia coli / Q000187:drug effects*; D006801:Humans; D042845:Laccase / Q000737:chemistry / Q000378:metabolism; D011124:Polyporaceae / Q000737:chemistry / Q000378:metabolism; D013211:Staphylococcus aureus / Q000187:drug effects*
D000890:Anti-Infective Agents; D000975:Antioxidants; D042845:Laccase