Ultra high performance liquid chromatography with ion-trap TOF-MS for the fast characterization of flavonoids in Citrus bergamia juice.
We have developed a fast ultra HPLC with ion-trap TOF-MS method for the analysis of flavonoids in Citrus bergamia juice. With respect to the typical methods for the analysis of these matrices based on conventional HPLC techniques, a tenfold faster separation was attained. The use of a core-shell particle column ensured high resolution within the fast analysis time of only 5 min. Unambiguous determination of flavonoid identity was obtained by the employment of a hybrid ion-trap TOF mass spectrometer with high mass accuracy (average error 1.69 ppm). The system showed good retention time and peak area repeatability, with maximum RSD% values of 0.36 and 3.86, respectively, as well as good linearity (R(2) ≥ 0.99). Our results show that ultra HPLC can be a useful tool for ultra fast qualitative/quantitative analysis of flavonoid compounds in citrus fruit juices.
Department of Pharmaceutical and Biomedical Sciences, University of Salerno, Fisciano, (SA), Italy.
Eduardo
E
Sommella
Giacomo
G
Pepe
Francesco
F
Pagano
Gian Carlo
GC
Tenore
Paola
P
Dugo
Michele
M
Manfra
Pietro
P
Campiglia
D001628:Beverages / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002957:Citrus / Q000737:chemistry*; D005419:Flavonoids / Q000737:chemistry*; D005638:Fruit / Q000737:chemistry; D013058:Mass Spectrometry / Q000379:methods*; D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry*
D005419:Flavonoids; D010936:Plant Extracts
Bergamot; Citrus; Core-Shell; Flavonoids; Mass accuracy