Graft union formation in artichoke grafting onto wild and cultivated cardoon: an anatomical study.
In order to develop a non-chemical method such as grafting effective against well-known artichoke soil borne diseases, an anatomical study of union formation in artichoke grafted onto selected wild and cultivated cardoon rootstocks, both resistant to Verticillium wilt, was performed. The cardoon accessions Belgio (cultivated cardoon) and Sardo (wild cardoon) were selected as rootstocks for grafting combinations with the artichoke cv. Romolo. Grafting experiments were carried out in the autumn and spring. The anatomical investigation of grafting union formation was conducted by scanning electron microscopy (SEM) on the grafting portions at the 3rd, 6th, 10th, 12th day after grafting. For the autumn experiment only, SEM analysis was also performed at 30 d after grafting. A high affinity between artichoke scion and cardoon rootstocks was observed, with some genotype differences in healing time between the two bionts. SEM images of scion/rootstock longitudinal sections revealed the appearance of many interconnecting structures between the two grafting components just 3d after grafting, followed by a vascular rearrangement and a callus development during graft union formation. De novo formation of many plasmodesmata between scion and rootstock confirmed their high compatibility, particularly in the globe artichoke/wild cardoon combination. Moreover, the duration of the early-stage grafting process could be influenced not only by the scion/rootstock compatibility, but also by the seasonal conditions, being favored by lower temperatures and a reduced light/dark photoperiod.
Consiglio per la Ricerca e la Sperimentazione in Agricoltura - Centro di Ricerca per lo Studio delle Relazioni tra Pianta e Suolo, Via della Navicella 2, 00184 Roma, Italy. Electronic address: alessandra.trinchera@entecra.it.
Alessandra
A
Trinchera
Gianmarco
G
Pandozy
Simona
S
Rinaldi
Paola
P
Crinò
Olindo
O
Temperini
Elvira
E
Rea
D000383:Agriculture; D031943:Cynara scolymus / Q000033:anatomy & histology* / Q000254:growth & development* / Q000648:ultrastructure; D012621:Seasons; D046509:Tissue Culture Techniques / Q000379:methods*
A; BSE; C; CC; EHT; Grafting compatibility; Plasmodesmata; R; R/CC; R/R; R/WC; Romolo; Romolo/Belgio; Romolo/Romolo; Romolo/Sardo; Rootstock/scion section; SE; SEM; Scanning electron microscopy; Tongue grafting; VP; WC; WD; artichoke; back-scattered electron; c; callus; cardoon; cultivated cardoon; electron high tension; gj; graft junction; hb; horizontal bridges; p; plasmodesmata; scanning electron microscopy; secondary electron; variable pressure; wild cardoon; working distance