SEF14 fimbriae from Salmonella enteritidis play a role in pathogenitic to cell model in vitro and host in vivo.
The role of SEF14 fimbriae in virulence remains to be elucidated and in this study, we showed that sefA mutant constructed in the wild-type (WT) Salmonella enteritidis strain 50336 displayed increased invasion to IPEC-J2 cell lines and survival in mouse peritoneal macrophages, and the lethal dose 50% (LD50) in 6-week-old Balb/c mice intra-peritoneally injected with WT S. enteritidis strain decreased significantly upon deletion of sefA indicating their role in virulence. Overall, these results demonstrated that expression of sefA of SEF14 fimbriae enhances S. enteritidis adhesion to epithelial cells and survival in macrophages and contributes to S. enteritidis virulence in mice.
Jiangsu Institute of Poultry Science, Yangzhou 225125, China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, China.
Chunhong
C
Zhu
Xia
X
Meng
Xiaoli
X
Duan
Zhiyun
Z
Tao
Jiansen
J
Gong
Huayan
H
Hou
Guoqiang
G
Zhu
D000818:Animals; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000382:microbiology; D036781:Fimbriae Proteins / Q000378:metabolism*; D007928:Lethal Dose 50; D017737:Macrophages, Peritoneal / Q000382:microbiology; D051379:Mice; D008807:Mice, Inbred BALB C; D012481:Salmonella Infections, Animal; D012477:Salmonella enteritidis / Q000472:pathogenicity*; D013552:Swine; D014774:Virulence; D037521:Virulence Factors / Q000378:metabolism*
D037521:Virulence Factors; C080931:sefA protein, Salmonella enteritidis; D036781:Fimbriae Proteins
Pathogenesis; SEF14 fimbriae; Salmonella enteritidis; sefA gene