Climate drives vein anatomy in Proteaceae.
OBJECTIVE
The mechanisms by which plants tolerate water deficit are only just becoming clear. One key factor in drought tolerance is the ability to maintain the capacity to conduct water through the leaves in conditions of water stress. Recent work has shown that a simple feature of the leaf xylem cells, the cube of the thickness of cell walls divided by the lumen width (t/b)(3), is strongly correlated with this ability.


METHODS
Using ecologically, phylogenetically, and anatomically diverse members of Proteaceae, we tested the relationships between (t/b)(3) and climate, leaf mass per unit area, leaf area, and vein density. To test relationships at high phylogenetic levels (mostly genus), we used phylogenetic and nonphylogenetic single and multiple regressions based on data from 50 species. We also used 14 within-genus species pairs to test for relationships at lower phylogenetic levels.


RESULTS
All analyses revealed that climate, especially mean annual precipitation, was the best predictor of (t/b)(3). The variation in (t/b)(3) was driven by variation in both lumen diameter and wall thickness, implying active control of these dimensions. Total vein density was weakly related to (t/b)(3) but unrelated to either leaf area or climate.


CONCLUSIONS
We conclude that xylem reinforcement is a fundamental adaptation for water stress tolerance and, among evergreen woody plants, drives a strong association between rainfall and xylem anatomy. The strong association between (t/b)(3) and climate cannot be explained by autocorrelation with other aspects of leaf form and anatomy that vary along precipitation gradients.
School of Plant Science, University of Tasmania, Private Bag 55, Hobart, Tasmania 7001, Australia. greg.jordan@utas.edu.au
Gregory J
GJ
Jordan
Timothy J
TJ
Brodribb
Christopher J
CJ
Blackman
Peter H
PH
Weston
D000222:Adaptation, Physiological; D002980:Climate; D055864:Droughts; D017753:Ecosystem; D010641:Phenotype; D018515:Plant Leaves / Q000033:anatomy & histology / Q000502:physiology; D039105:Proteaceae / Q000033:anatomy & histology* / Q000502:physiology; D011891:Rain; D012044:Regression Analysis; D014867:Water / Q000502:physiology; D014934:Wood / Q000033:anatomy & histology / Q000502:physiology; D052584:Xylem / Q000033:anatomy & histology* / Q000502:physiology
D014867:Water
Proteaceae; anatomy; climate; collapse; hydraulic vulnerability; vein density; xeromorphy; xylem