LEGO-NMR spectroscopy: a method to visualize individual subunits in large heteromeric complexes.
Seeing the big picture: Asymmetric macromolecular complexes that are NMR active in only a subset of their subunits can be prepared, thus decreasing NMR spectral complexity. For the hetero heptameric LSm1-7 and LSm2-8 rings NMR spectra of the individual subunits of the complete complex are obtained, showing a conserved RNA binding site. This LEGO-NMR technique makes large asymmetric complexes accessible to detailed NMR spectroscopic studies.
Max Planck Institute for Developmental Biology, Spemannstrasse 35, 72076 Tübingen (Germany); Present address: European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg (Germany).
Markus
M
Mund
Jan H
JH
Overbeck
Janina
J
Ullmann
Remco
R
Sprangers
D046911:Macromolecular Substances; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008958:Models, Molecular; D020836:Protein Structure, Quaternary
D046911:Macromolecular Substances
LSm complex; NMR spectroscopy; isotopic labeling; methyl TROSY; protein structures