[Development and identification of monoclonal antibodies of cape jasmine proteins in Reduning injection].
Liposoluble cape jasmine proteins were used to immunize BALB/C mice. Indirect ELISA was utilized to develop one monoclonal antibody by integrating SP2/0 cells and spleen cells from immunized BALB/C mice. The subclass of the monoclonal antibody was identified as IgG2b, with Kappa chain as its light chain. The ascite titer of 2H8 monoclonal antibody was 1:204 080. Western-blot analysis proved that 2H8 reacted with cape jasmine proteins to identify specific liposoluble protein with molecuar weight of around 58.5 kDa. Dot-ELISA was established with 2H8 ascites as the primary antibody, showing the minimum detectable amount of 19.5 ng. This study lays a foundation for the development of protein kits of Reduning injection.
Jiangsu Kanion Pharmaceutical Co., Ltd., State Key Laboratory of New Pharmaceutical Process for Traditional Chinese Medicine, Enterprises Academician Workstations in Jiangsu Province, Lianyungang 222001, China. lf198098@126.com
Fang
F
Li
Jian-Ming
JM
Zhou
Hong-Mei
HM
Wang
Jun
J
Zhou
Yu-An
YA
Bi
Zhen-Zhong
ZZ
Wang
Wei
W
Xiao
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis / Q000276:immunology*; D000918:Antibody Specificity; D004342:Drug Hypersensitivity / Q000276:immunology*; D004365:Drugs, Chinese Herbal / Q000009:adverse effects* / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005260:Female; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D010940:Plant Proteins / Q000032:analysis / Q000276:immunology*; D011933:Reagent Kits, Diagnostic
D000911:Antibodies, Monoclonal; D004365:Drugs, Chinese Herbal; D010940:Plant Proteins; D011933:Reagent Kits, Diagnostic