A universal real-time PCR assay for rapid quantification of microRNAs via the enhancement of base-stacking hybridization.
Via the base-stacking hybridization strategy, we have developed a universal, one-step real-time quantitative PCR assay for sensitive and selective detection of microRNAs. This proposed assay has several intrinsic features including rapid response, low cost, simple handling procedures, etc.
Lab of Biosystem and Microanalysis, East China University of Science & Technology, Shanghai 200237, PR China.
Cui-Yuan
CY
Yu
Bin-Cheng
BC
Yin
Bang-Ce
BC
Ye
D020029:Base Pairing; D045744:Cell Line, Tumor; D006367:HeLa Cells; D006801:Humans; D035683:MicroRNAs / Q000032:analysis* / Q000737:chemistry / Q000235:genetics; D021141:Nucleic Acid Amplification Techniques; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity
D035683:MicroRNAs