Enhanced interpretation of adsorption data generated by liquid chromatography and by modern biosensors.
In this study we demonstrate the importance of proper data processing in adsorption isotherm estimations. This was done by investigating and reprocessing data from five cases on two closely related platforms: liquid chromatography (LC) and biosensors. The previously acquired adsorption data were reevaluated and reprocessed using a three-step numerical procedure: (i) preprocessing of adsorption data, (ii) adsorption data analysis and (iii) final rival model fit. For each case, we will discuss what we really measure and what additional information can be obtained by numerical processing of the data. These cases clearly demonstrate that numerical processing of LC and biosensor data can be used to gain deeper understanding of molecular interactions with adsorption media. This is important because adsorption data, especially from biosensors, is often processed using old and simplified methods.
Department of Chemistry - BMC, Uppsala University, P.O. Box 579, SE-751 23 Uppsala, Sweden.
Víctor
V
Agmo Hernández
Jörgen
J
Samuelsson
Patrik
P
Forssén
Torgny
T
Fornstedt
D000327:Adsorption; D002853:Chromatography, Liquid; D005470:Fluorometry; D058525:Quartz Crystal Microbalance Techniques; D020349:Surface Plasmon Resonance; D013816:Thermodynamics
Adsorption energy distribution; Adsorption isotherm; Data processing; Fluorescence; Quartz Crystal Microbalance; Surface Plasmon Resonance