In vitro evaluation of schistosomicidal activity of essential oil of Mentha x villosa and some of its chemical constituents in adult worms of Schistosoma mansoni.
This study aimed to determine the composition of the essential oil of Mentha x villosa and to evaluate its biological effects in vitro on adult worms of S. mansoni. Rotundifolone (70.96 %), limonene (8.75 %), trans-caryophyllene (1.46 %), and β-pinene (0.81 %) were shown to be the major constituents of this oil. Adult worms of S. mansoni were incubated with different concentrations of the essential oil (1, 10, 100, 250, 500, and 1000 µg/mL) and of its constituents rotundifolone (0.7, 3.54, 7.09, 70.96, 177.4, 354.8, and 700.96 µg/mL), limonene (43.75 µg/mL), trans-caryophyllene (7.3 µg/mL), and β-pinene (4.03 µg/mL). No schistosomicidal activity was identified at the trans-caryophyllene and β-pinene concentrations studied. However, use of the essential oil (10 µg/mL), rotundifolone (7.09 µg/mL), and limonene (43.75 µg/mL) resulted in decreased worm motility continuing until 96 hours of observation. At higher concentrations (100 and 70.96 µg/mL, respectively), both the essential oil and rotundifolone caused mortality among adult worms of S. mansoni. The positive control praziquantel caused the death of all parasites after 24 h of evaluation. The results from this study suggest that the essential oil of Mentha x villosa presents schistosomicidal efficacy.
Molecular and Cell Biology Laboratory, Department of Parasitology, Aggeu Magalhães Research Center, Oswaldo Cruz Foundation, Recife, Pernambuco, Brazil.
Thiago José
TJ
Matos-Rocha
Marília Gabriela
MG
dos Santos Cavalcanti
José Maria
JM
Barbosa-Filho
Ana Silvia Suassuna Carneiro
AS
Lúcio
Dyana Leal
DL
Veras
Ana Paula Sampaio
AP
Feitosa
José Pinto
JP
de Siqueira Júnior
Reinaldo Nóbrega
RN
de Almeida
Márcia Ortiz Mayo
MO
Marques
Luiz Carlos
LC
Alves
Fábio André
FA
Brayner
D000818:Animals; D000081008:Bicyclic Monoterpenes; D001643:Bridged Bicyclo Compounds / Q000032:analysis / Q000494:pharmacology; D053138:Cyclohexenes / Q000032:analysis / Q000494:pharmacology; D000077222:Limonene; D027541:Mentha / Q000737:chemistry*; D039821:Monoterpenes / Q000032:analysis / Q000494:pharmacology; D009822:Oils, Volatile / Q000737:chemistry / Q000494:pharmacology*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D000081223:Polycyclic Sesquiterpenes; D012550:Schistosoma mansoni / Q000187:drug effects*; D012556:Schistosomicides / Q000494:pharmacology*; D012717:Sesquiterpenes / Q000032:analysis / Q000494:pharmacology; D013729:Terpenes / Q000032:analysis / Q000494:pharmacology
D000081008:Bicyclic Monoterpenes; D001643:Bridged Bicyclo Compounds; D053138:Cyclohexenes; D039821:Monoterpenes; D009822:Oils, Volatile; D010936:Plant Extracts; D000081223:Polycyclic Sesquiterpenes; D012556:Schistosomicides; D012717:Sesquiterpenes; D013729:Terpenes; C010789:beta-pinene; C467828:rotundifolone; D000077222:Limonene; C024714:caryophyllene