[HPLC fingerprints establishment of chemical constituents in genus Chimonanthus leaves].
This study was aimed to establish the HPLC fingerprints of the genus Chimonanthus leaves and compare the constituents distribution among five Chimonanthus species . The analysis was conducted on a C15 column (4.6 mm x 250 mm, 5 microm) with the mobile phase containing acetonitrile-water in gradient program: acetonitrile (B), 0-20 min, 6%-20%; 20-30 min, 20%-25%; 30-40 min, 25%-45%; 40-50 min, 45%-80%; 50-80 min, 80%-85%; 80-90 min, 85%-100%; 90-110 min, 100%. Flow rate was 0.8 mL x min(-1) and detection wavelength was 228 nm. Column temperature was set at 30 degrees C. The HPLC fingerprints of the five Chimonanthus species have been established. Ch. praecox, Ch. nitens, Ch. salicifolius, Ch. Zhejiangensis and Ch. grammatus have significant difference in constituents distribution and contents. Five standard substances as common compounds were confirmed in chromatography fingerprints. The method can be used as quality evaluation and classicfication of the genus Chimonanthus.
The Nurturing Station for the State Key Laboratory of Subtropical Silviculture, Research and Development Center for Natural Medicine, Zhejiang Agricultural and Forestry University, Lin'an 311300, China.
Hong-Tao
HT
Liu
Ming-Pan
MP
Cao
Xin-Feng
XF
Zhang
Jin-Ping
JP
Si
D031237:Calycanthaceae / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000032:analysis*; D018515:Plant Leaves / Q000737:chemistry
D004365:Drugs, Chinese Herbal