Detoxification of aflatoxin B1 by an aqueous extract from leaves of Adhatoda vasica Nees.
The effectiveness of aqueous extracts of various medicinal plants in detoxification of aflatoxin B1 (AFB1) was tested in vitro by thin-layer chromatography and enzyme-linked immunosorbent assay (ELISA). Among the different plant extracts, the leaf extract of Vasaka (Adhatoda vasica Nees) showed the maximum degradation of AFB1 (≥ 98%) after incubation for 24h at 37 °C. The aflatoxin detoxifying activity of the A. vasica leaf extract was significantly reduced by heating to 100 °C for 10 min or autoclaving at 121 °C for 20 min. Dialysis had no effect on aflatoxin detoxifying ability of A. vasica extract and the dialyzed extract showed similar level of detoxification of AFB1 as that of the untreated extract. A time course study of aflatoxin detoxification by A. vasica extract showed that 69% of the toxin was degraded within 6h and ≥ 95% degradation was observed after 24h of incubation. Detoxification of AFB1 by A. vasica extract was further confirmed by liquid chromatography-mass spectrometry (LC-MS) analysis. Phytochemical analysis revealed the presence of alkaloids in methanolic extract of A. vasica leaves. A partially purified alkaloid from A. vasica leaves by preparative TLC exhibited strong AFB1 detoxification activity.
Department of Plant Pathology, Centre for Plant Protection Studies, Tamil Nadu Agricultural University, Coimbatore 641 003, Tamil Nadu, India.
S
S
Vijayanandraj
Department of Food and Agricultural Processing Engineering, Agricultural Engineering College and Research Institute, Tamil Nadu Agricultural University, Coimbatore 641003, Tamil Nadu, India.
R
R
Brinda
Department of Plant Pathology, Centre for Plant Protection Studies, Tamil Nadu Agricultural University, Coimbatore 641 003, Tamil Nadu, India.
K
K
Kannan
Department of Plant Pathology, Centre for Plant Protection Studies, Tamil Nadu Agricultural University, Coimbatore 641 003, Tamil Nadu, India.
R
R
Adhithya
Department of Plant Pathology, Centre for Plant Protection Studies, Tamil Nadu Agricultural University, Coimbatore 641 003, Tamil Nadu, India.
S
S
Vinothini
Department of Agricultural Entomology, Centre for Plant Protection Studies, Tamil Nadu Agricultural University, Coimbatore 641 003, Tamil Nadu, India.
K
K
Senthil
Jawaharlal Nehru Technological University Hyderabad, Kukatpally, Hyderabad 500085, Andhra Pradesh, India.
Ramakoteswara Rao
RR
Chinta
Department of Plant Pathology, Centre for Plant Protection Studies, Tamil Nadu Agricultural University, Coimbatore 641 003, Tamil Nadu, India.
V
V
Paranidharan
Department of Plant Pathology, Centre for Plant Protection Studies, Tamil Nadu Agricultural University, Coimbatore 641 003, Tamil Nadu, India. Electronic address: velazhahan@hotmail.com.
R
R
Velazhahan
D016604:Aflatoxin B1 / Q000037:antagonists & inhibitors*; D000470:Alkaloids / Q000302:isolation & purification / Q000378:metabolism; D002853:Chromatography, Liquid; D002855:Chromatography, Thin Layer; D004797:Enzyme-Linked Immunosorbent Assay; D027902:Justicia / Q000737:chemistry*; D007700:Kinetics; D013058:Mass Spectrometry; D010936:Plant Extracts / Q000302:isolation & purification / Q000378:metabolism*; D018515:Plant Leaves / Q000737:chemistry; D013696:Temperature; D013997:Time Factors
D000470:Alkaloids; D010936:Plant Extracts; D016604:Aflatoxin B1
Adhatoda vasica; Aflatoxin B1; Detoxification; Medicinal plant