Determination of acarbose by capillary zone electrophoresis.
Acarbose (Glucobay, Bayer AG) acts as a potent alpha-glucosidase-inhibitor, which delays the intestinal starch digestion resulting in a reduction of postprandial blood glucose and insulin levels. Acarbose is a pseudo-tetrasaccharide, with two D-glucose units linked via an alpha 1-->4 glycosidic bond to acarviosin, which is a N-glycoside composed of an unsaturated cyclitol and 4-amino-4,6-dideoxy-alpha-D-glucopyranose. Several methods for the determination of acarbose by capillary electrophoresis can be found in literature. They are based either on the derivatisation with 7-aminonaphthalene-1,3-disulfonic acid (ANDS) or on the detection of the unsaturated cyclitol at wavelengths below 200 nm. The aim of our work was the determination of acarbose making use of a previously developed method based on reductive amination with S-phenylethylamine. The aminoalditols generated in the reaction formed differently charged borate-complexes depending on the configuration of the sugar. After successful method optimisation we were able to separate two potential impurities of acarbose, D-maltose und D-glucose. For the quantitation of acarbose in Glucobay tablets an additional borate-buffer system was established, reducing the total time of analysis to less than 10 min.
Department of Medicinal Chemistry, University of Vienna, Austria.
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D020909:Acarbose / Q000032:analysis*; D001881:Borates / Q000737:chemistry; D002021:Buffers; D002138:Calibration; D002626:Chemistry, Pharmaceutical; D019075:Electrophoresis, Capillary / Q000379:methods*; D004791:Enzyme Inhibitors / Q000032:analysis*; D065089:Glycoside Hydrolase Inhibitors; D007202:Indicators and Reagents; D010627:Phenethylamines / Q000737:chemistry; D012015:Reference Standards; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet
D001881:Borates; D002021:Buffers; D004791:Enzyme Inhibitors; D065089:Glycoside Hydrolase Inhibitors; D007202:Indicators and Reagents; D010627:Phenethylamines; D020909:Acarbose