Electric migration of α-hemolysin in supported n-bilayers: a model for transmembrane protein microelectrophoresis.
Proteome analysis involves separating proteins as a preliminary step toward their characterization. This paper reports on the translational migration of a model transmembrane protein (α-hemolysin) in supported n-bilayers (n, the number of bilayers, varies from 1 to around 500 bilayers) when an electric field parallel to the membrane plane is applied. The migration changes in direction as the charge on the protein changes its sign. Its electrophoretic mobility is shown to depend on size and charge. The electrophoretic mobility varies as 1/R(2), with R the equivalent geometric radius of the embedded part of the protein. Measuring mobilities at differing pH in our system enables us to determine the pI and the charge of the protein. Establishing all these variations points to the feasibility of electrophoretic transport of a charged object in this medium and is a first step toward electrophoretic separation of membrane proteins in n-bilayer systems.
Aix-Marseille Université, CINaM, CNRS, Marseille, France.
Frédéric
F
Harb
Bernard
B
Tinland
D001427:Bacterial Toxins / Q000737:chemistry* / Q000302:isolation & purification; D004058:Diffusion; D004560:Electricity; D004586:Electrophoresis / Q000295:instrumentation*; D004867:Equipment Design; D006460:Hemolysin Proteins / Q000737:chemistry* / Q000302:isolation & purification; D008051:Lipid Bilayers / Q000737:chemistry*; D013211:Staphylococcus aureus / Q000737:chemistry*
D001427:Bacterial Toxins; D006460:Hemolysin Proteins; D008051:Lipid Bilayers; C021091:staphylococcal alpha-toxin
Membrane; Mobility; Proteins; Separation