Plasma protein denaturation with graded heat exposure.
During cardiopulmonary bypass (CPB), perfusion at tepid temperatures (33-35 °C) is recommended to avoid high temperature cerebral hyperthermia during and after the operation. However, the ideal temperature for uncomplicated adult cardiac surgery is an unsettled question. Typically, the heat exchanger maximum temperature is monitored between 40-42 °C to prevent denaturation of plasma proteins, but studies have not been performed to make these conclusions. Therefore, our hypothesis was to determine the temperature in which blood plasma protein degradation occurs after 2 hours of heat exposure. As a result, blood plasma proteins were exposed to heat in the 37-50 °C range for 2 hours. Plasma protein samples were loaded onto an 8-12% gradient gel for SDS-PAGE and low molecular weight plasma protein degradation was detected with graded heat exposure. Protein degradation was first detected between 43-45 °C of heat exposure. This study supports the practice of monitoring the heat exchanger between 40-42 °C to prevent denaturation of plasma proteins.
Sarver Heart Center, College of Medicine, The University of Arizona, Tucson, AZ.
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R
Vazquez
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DF
Larson
D000818:Animals; D001798:Blood Proteins / Q000378:metabolism*; D002315:Cardiopulmonary Bypass / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D006358:Hot Temperature; D051379:Mice; D008810:Mice, Inbred C57BL; D010477:Perfusion / Q000379:methods; D011489:Protein Denaturation
D001798:Blood Proteins
SDS-PAGE; degradation; denaturation; heat exposure; plasma protein