Antioxidant and genoprotective effects of spent coffee extracts in human cells.
Spent coffee has been shown as a good source of hydrophilic antioxidant compounds. The ability of two spent coffee extracts rich in caffeoylquinic acids, mainly dicaffeoylquinic acids, and caffeine (Arabica filter and Robusta espresso) to protect against oxidation and DNA damage in human cells (HeLa) was evaluated at short (2 h) and long (24 h) exposure times. Cell viability (MTT) was not affected by spent coffee extracts (>80%) up to 1000 μg/mL after 2 h. Both spent coffee extracts significantly reduced the increase of ROS level and DNA strand breaks (29-73% protection by comet assay) induced by H₂O₂. Pretreatment of cells with robusta spent coffee extract also decreased Ro photosensitizer-induced oxidative DNA damage after 24 h exposure. The higher effectiveness of Robusta spent coffee extract, with less caffeoylquinic acids and melanoidins, might be due to other antioxidant compounds, such as caffeine and other Maillard reaction products. This work evidences the potential antioxidant and genoprotective properties of spent coffee in human cells.
Department of Nutrition, Food Science and Physiology, School of Pharmacy, University of Navarra, E-31008 Pamplona, Spain.
Jimena
J
Bravo
Leire
L
Arbillaga
M Paz
MP
de Peña
Concepcion
C
Cid
D016587:Antimutagenic Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D002110:Caffeine / Q000032:analysis / Q000494:pharmacology; D002470:Cell Survival / Q000187:drug effects; D003069:Coffee / Q000737:chemistry*; D020552:Comet Assay; D004249:DNA Damage / Q000187:drug effects*; D006367:HeLa Cells; D006801:Humans; D006861:Hydrogen Peroxide / Q000009:adverse effects; D015416:Maillard Reaction; D018384:Oxidative Stress / Q000187:drug effects; D010936:Plant Extracts / Q000494:pharmacology*; D011801:Quinic Acid / Q000031:analogs & derivatives / Q000032:analysis / Q000494:pharmacology; D017382:Reactive Oxygen Species
D016587:Antimutagenic Agents; D000975:Antioxidants; D003069:Coffee; D010936:Plant Extracts; D017382:Reactive Oxygen Species; C472707:caffeoylquinic acid; D011801:Quinic Acid; D002110:Caffeine; D006861:Hydrogen Peroxide
(3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyltetrazolium bromide); 2′,7′-dichlorfluorescein-diacetate; 4,6-diamidino-2-phenylindole; 8-oxoGua; 8-oxoguanine; Antigenotoxicity; Antioxidant; CQAs; Coffee; Comet assay; DAPI; DNA damage; FPG; H(2)DCF-DA; H(2)O(2); MRPs; MTT; Maillard reaction products; ROS; SBs; Spent coffee; caffeoylquinic acids; diCQAs; dicaffeoylquinic acids; formamidopyrimidine DNA glycosylase; hydrogen peroxide; reactive oxygen species; strand breaks