Effect of intermittent low-frequency electrical stimulation on the rat gastrocnemius muscle.
Low-frequency neuromuscular electrical stimulation (NMES) has been used as an endurance exercise model. This study aimed to test whether low-frequency NMES increases the phosphorylation of anabolic signaling molecules and induces skeletal muscle hypertrophy, as seen with high-frequency NMES. Using Sprague-Dawley rats, 1 bout of exercise (with dissection done immediately (Post0) and 3 h (Post3) after exercise) and another 6 sessions of training were performed. All experimental groups consisted of high- and low-frequency stimulation (HFS: 100 Hz; LFS: 10 Hz). Periodic acid-Schiff (PAS) staining was conducted to investigate type II fiber activation, and western blot analysis (WB) was conducted to examine whether NMES leads to anabolic intracellular signaling. At first, we examined the acute effect of exercise. PAS staining revealed that glycogen depletion occurred in both type I and type II fibers. WB results demonstrated that p70S6K phosphorylation was significantly increased by HFS, but there was no significant difference with LFS. In contrast, ERK 1/2 phosphorylation was increased by LFS at Post0. In the 6-session training, the wet weight and myofibrillar protein were significantly increased by both HFS and LFS. In conclusion, LFS has a similar anabolic effect for skeletal muscle hypertrophy as HFS, but the mediating signaling pathway might differ.
Graduate School of Health and Sport Science, Nippon Sport Science University, Tokyo 158-8508, Japan. tsutaki.a@gmail.com
Arata
A
Tsutaki
Riki
R
Ogasawara
Koji
K
Kobayashi
Kihyuk
K
Lee
Karina
K
Kouzaki
Koichi
K
Nakazato
D000818:Animals; D004558:Electric Stimulation; D006984:Hypertrophy / Q000473:pathology; D048052:Mitogen-Activated Protein Kinase 3 / Q000378:metabolism; D009124:Muscle Proteins / Q000378:metabolism; D018482:Muscle, Skeletal / Q000528:radiation effects*; D010766:Phosphorylation; D010805:Physical Conditioning, Animal; D010807:Physical Endurance / Q000528:radiation effects; D051381:Rats; D015398:Signal Transduction / Q000528:radiation effects
D009124:Muscle Proteins; D048052:Mitogen-Activated Protein Kinase 3