Response surface modeling and optimization of ultrasound-assisted extraction of three flavonoids from tartary buckwheat (Fagopyrum tataricum).
BACKGROUND
Buckwheat (Fagopyrum spp., Polygonaceae) is a widely planted food crop. Flavonoids, including quercetin, rutin, and kaempferol, are the main bioactive components in tartary buckwheat (Fagopyrum tataricum (L.) Gaertn). From the nutriological and pharmacological perspectives, flavonoids have great value in controlling blood glucose and blood pressure levels, and they also have antioxidant properties.


OBJECTIVE
To optimize the conditions for extraction of quercetin, rutin, and kaempferol from F. tataricum.


METHODS
A combination of ultrasound-assisted extraction (UAE) and response surface methodology (RSM) was used for flavonoid extraction and yield assessment. The RSM was based on a three-level, three-variable Box-Behnken design.


RESULTS
Flavonoids were optimally extracted from F. tataricum by using 72% methanol, at 60°C, for 21 minutes. Under these conditions, the obtained extraction yield of the total flavonoids was 3.94%.


CONCLUSIONS
The results indicated that the UAE method was effective for extraction of flavonoids from tartary buckwheat.
College of Biotechnology Industry, Chengdu University, Chengluo Road, Longquanyi District, Chengdu 610106, Sichuan, China.
Lian-Xin
LX
Peng
Liang
L
Zou
Jiang-Lin
JL
Zhao
Da-Bing
DB
Xiang
Peng
P
Zhu
Gang
G
Zhao
Buckwheat; Fagopyrum tataricum; flavonoids; response surface methodology; ultrasound-assisted extraction