Beneficial effect of rutin on oxonate-induced hyperuricemia and renal dysfunction in mice.
OBJECTIVE
The aim of the present study was to investigate the effects of rutin on potassium oxonate-induced hyperuricemia and renal dysfunction in mice.


METHODS
Rutin was administered orally 1 h after oxonate at doses of 25, 50 and 100 mg·kg(-1). Serum uric acid levels and kidney function parameters were assayed. Mouse uromodulin levels in serum, urine and kidney were determined by the ELISA method. Simultaneously, the expression levels of renal organic ion transporters were detected.


RESULTS
Rutin significantly decreased serum urate, creatinine and blood urea nitrogen, serum and kidney uromodulin levels, and increased urine uromodulin, urate and creatinine excretion in hyperuricemic mice. Rutin at 50 and 100 mg·kg(-1) significantly downregulated mRNA and protein levels of mouse glucose transporter 9 and urate transporter 1, and upregulated mRNA and protein levels of organic anion transporter 1 and organic cation/carnitine transporters in the kidney of hyperuricemic mice.


CONCLUSIONS
Rutin exerted its hypouricemic action and renal function improvement by the regulation of renal organic ion transporters.
State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing, PR China.
Yu-Sheng
YS
Chen
Qing-Hua
QH
Hu
Xian
X
Zhang
Qin
Q
Zhu
Ling-Dong
LD
Kong
D000818:Animals; D002352:Carrier Proteins / Q000378:metabolism; D051246:Glucose Transport Proteins, Facilitative / Q000235:genetics / Q000378:metabolism; D033461:Hyperuricemia / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism; D007674:Kidney Diseases / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism; D051379:Mice; D050811:Octamer Transcription Factor-1 / Q000378:metabolism; D027362:Organic Anion Transport Protein 1 / Q000235:genetics / Q000378:metabolism; D027361:Organic Anion Transporters / Q000235:genetics / Q000378:metabolism; D027701:Organic Cation Transport Proteins / Q000378:metabolism; D000075102:Organic Cation Transporter 2; D010094:Oxonic Acid; D012333:RNA, Messenger / Q000378:metabolism; D012431:Rutin / Q000494:pharmacology / Q000627:therapeutic use*; D000074058:Solute Carrier Family 22 Member 5; D027981:Symporters; D058949:Uromodulin / Q000235:genetics / Q000378:metabolism
D002352:Carrier Proteins; D051246:Glucose Transport Proteins, Facilitative; D008565:Membrane Proteins; D050811:Octamer Transcription Factor-1; D027362:Organic Anion Transport Protein 1; D027361:Organic Anion Transporters; D027701:Organic Cation Transport Proteins; D000075102:Organic Cation Transporter 2; C494166:Pou2f1 protein, mouse; D012333:RNA, Messenger; C109687:Slc22a12 protein, mouse; C491012:Slc22a2 protein, mouse; C491659:Slc22a4 protein, mouse; C492019:Slc22a5 protein, mouse; C105033:Slc22a6 protein, mouse; C498775:Slc2a9 protein, mouse; D000074058:Solute Carrier Family 22 Member 5; D027981:Symporters; D058949:Uromodulin; D012431:Rutin; D010094:Oxonic Acid