Loss of PPARγ expression in mammary secretory epithelial cells creates a pro-breast tumorigenic environment.
Breast cancer is the leading cause of new cancer diagnoses among women. Using peroxisome proliferator-activated receptor (PPAR)γ((+/-)) mice, we showed normal expression of PPARγ was critical to stop 7,12-dimethylbenz[a]anthracene (DMBA)-induced breast tumorigenesis. PPARγ is expressed in many breast cell types including mammary secretory epithelial (MSE) cells. MSEs proliferate as required during pregnancy, and undergo apoptosis or reversible transdifferentiation during involution once lactation is complete. Thus, MSE-specific loss of PPARγ was hypothesized to enhance DMBA-mediated breast tumorigenesis. To test this, MSE cell-specific PPARγ knockout (PPARγ-MSE KO) and control (PPARγ-WT) mice were generated, mated and allowed to nurse for three days. One week after involution, dams were treated with DMBA to initiate breast tumors, and randomized on week 7 to continue receiving a normal chow diet (DMBA Only: PPARγ-WT, n = 15; PPARγ-MSE KO, n = 25) or one supplemented with a PPARγ activating drug (DMBA + ROSI: PPARγ-WT, n = 17; PPARγ-MSE KO, n = 24), and monitored for changes in breast tumor outcomes. PPARγ-MSE KOs had significantly lower overall survival and decreased mammary tumor latency as compared to PPARγ-WT controls. PPARγ activation significantly reduced DMBA-mediated malignant mammary tumor volumes irrespective of genotype. MSE-specific PPARγ loss resulted in decreased mammary gland expression of PTEN and Bax, increased superoxide anion production, and elevated serum eotaxin and RANTES, creating a protumorigenic environment. Moreover, PPARγ activation in MSEs delayed mammary tumor growth in part by down-regulating Cox-1, Cox-2 and cyclin D1. Collectively, these studies highlight a protective role of MSE-specific PPARγ during breast tumorigenesis, and support a novel chemotherapeutic role of PPARγ activation in breast cancer.
Department of Pathology and Molecular Medicine, Queen's University, Kingston, ON, Canada.
Anthony J
AJ
Apostoli
Graham E A
GE
Skelhorne-Gross
Rachel E
RE
Rubino
Nichole T
NT
Peterson
Michael A
MA
Di Lena
Mark M
MM
Schneider
Sandip K
SK
SenGupta
Christopher J B
CJ
Nicol
D015127:9,10-Dimethyl-1,2-benzanthracene; D000818:Animals; D004847:Epithelial Cells / Q000378:metabolism; D005260:Female; D008321:Mammary Glands, Animal / Q000378:metabolism*; D008325:Mammary Neoplasms, Experimental / Q000209:etiology* / Q000378:metabolism; D051379:Mice; D018345:Mice, Knockout; D047495:PPAR gamma / Q000502:physiology*; D051059:PTEN Phosphohydrolase / Q000032:analysis; D051028:bcl-2-Associated X Protein / Q000032:analysis
C494827:Bax protein, mouse; D047495:PPAR gamma; D051028:bcl-2-Associated X Protein; D015127:9,10-Dimethyl-1,2-benzanthracene; D051059:PTEN Phosphohydrolase; C487757:Pten protein, mouse
PPARγ; breast cancer; chemical carcinogenesis; chemotherapy; knockout mouse model; mammary secretory epithelial cells