Biomarkers and prognosis after R0 resection of colorectal liver metastases.
BACKGROUND
Evaluation of biomarkers and clinical factors associated with cancer-specific survival after curative resection for colorectal cancer liver metastases (LM).


METHODS
All patients who had an R0 resection for LM between 2000-2006 were reviewed. Clinical and histological data were assessed; p53 expression was studied by IHC. ERCC1 codon 118 and XRCC1 codon 399 were analyzed by PCR-RFLP using BsrDI and HpaII, respectively.


RESULTS
Out of 119 patients included (80 synchronous LM (67.2%), median number 2 (1-18)), 104 patients (87.4%) received chemotherapy before recurrence; 60 patients (50.4%) had a p53 negative tumor. ERCC1 distribution was: 31(26%) AAC/AAC, 44(37%) AAC/AAT and 44(37%) AAT/AAT. XRCC1 distribution was: 46(39%) CGG/CGG, 53(44.9%) CGG/CAG and 19(16.1%) CAG/CAG. Three and 5-years disease free survival (DFS) and overall survival (OS) were 31%, 22.7%, 77.4%, and 66.6%, respectively. Node ratio >0.2 (p = 0.0042), LM number >3 (p <0.0001), bilobar localization (p = 0.0074) and preoperative chemotherapy (p = 0.0036) were associated with a shorter DFS. None of the biomarkers was found to influence DFS. In multivariate analysis, a number of LM >3 was the only independent factor. No factor was found to influence OS.


CONCLUSIONS
The studied biomarkers had no significant impact on prognosis. For routine practice, clinical factors remain the only usable available tools.
François
F
Radais
Aziz
A
Zaanan
Jérémie H
JH
Lefévre
Jean-Francois
JF
Fléjou
Pierre
P
Balladur
François
F
Paye
Emmanuel
E
Tiret
Alex
A
Duva
Francoise
F
Praz
Yann
Y
Parc
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015415:Biomarkers; D003062:Codon; D015179:Colorectal Neoplasms / Q000473:pathology*; D004268:DNA-Binding Proteins / Q000235:genetics; D004720:Endonucleases / Q000235:genetics; D005260:Female; D006498:Hepatectomy; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics / Q000401:mortality / Q000556:secondary* / Q000601:surgery*; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic; D011379:Prognosis; D016159:Tumor Suppressor Protein p53 / Q000032:analysis; D000076105:X-ray Repair Cross Complementing Protein 1
D015415:Biomarkers; D003062:Codon; D004268:DNA-Binding Proteins; D016159:Tumor Suppressor Protein p53; D000076105:X-ray Repair Cross Complementing Protein 1; C000619371:XRCC1 protein, human; C071447:ERCC1 protein, human; D004720:Endonucleases