Microcalorimetry assay for rapid detection of voriconazole resistance in Aspergillus fumigatus.
We describe a calorimetric assay for detection of voriconazole-resistant Aspergillus fumigatus within 8 h. Among 27 genetically distinct strains, all 21 resistant and all 6 susceptible strains were correctly identified by measurement of fungal heat production in the presence of voriconazole. This proof-of-concept study demonstrates the potential of microcalorimetry for rapid detection of azole resistance in A. fumigatus.
Unit of Septic Surgery, Lausanne University Hospital, Lausanne, Switzerland.
Ulrika
U
Furustrand Tafin
Jacques F
JF
Meis
Andrej
A
Trampuz
D000935:Antifungal Agents / Q000494:pharmacology*; D001232:Aspergillus fumigatus / Q000187:drug effects* / Q000254:growth & development; D002151:Calorimetry / Q000379:methods; D025141:Drug Resistance, Fungal; D008826:Microbial Sensitivity Tests; D011743:Pyrimidines / Q000494:pharmacology*; D013816:Thermodynamics; D013997:Time Factors; D014230:Triazoles / Q000494:pharmacology*; D065819:Voriconazole
D000935:Antifungal Agents; D011743:Pyrimidines; D014230:Triazoles; D065819:Voriconazole