Association of progesterone receptor gene polymorphism with male infertility and clinical outcome of ICSI.
OBJECTIVE
To investigate the association of Progesterone Receptor (PR) gene variations and male infertility


METHODS
DNA extraction, PCR and sequencing of PR gene, PROGINS insertion by PCR. Association of the variations with seminal parameters and outcomes of ICSI.


RESULTS
Four known SNPs in the PR gene were identified in the study of which three (rs3740753, rs1042838, rs104283) were co-inherited and in complete linkage disequilibrium with the PROGINS Alu insertion. There were no differences in their frequencies between fertile and infertile males. The rs2020880 was found at a very low frequency only in the controls but not in the infertile subjects. The sperm counts, fertilization rate, embryo quality or pregnancy rates were not different in individuals with or without PROGINS allele.


CONCLUSIONS
PR gene alterations are not associated with male infertility or ICSI outcome.
National Institute for Research in Reproductive Health (ICMR), J. M. Street, Parel, Mumbai, 400012, Maharashtra, India.
Sanjukta
S
Sen
Abhijit
A
Dixit
Chitra
C
Thakur
Jyotsna
J
Gokral
Indira
I
Hinduja
Kusum
K
Zaveri
Kumarasamy
K
Thangaraj
Deepak
D
Modi
D000483:Alleles; D005260:Female; D005787:Gene Frequency; D056726:Genetic Association Studies; D006801:Humans; D054643:INDEL Mutation / Q000235:genetics; D007248:Infertility, Male / Q000235:genetics*; D015810:Linkage Disequilibrium; D008297:Male; D011247:Pregnancy; D018873:Pregnancy Rate; D011980:Receptors, Progesterone / Q000235:genetics*; D020554:Sperm Injections, Intracytoplasmic
D011980:Receptors, Progesterone