Interaction of lobed kudzuvine root, rhizoma chuanxiong with both acetylcholinesterase and beta-amyloid (Aβ1-42).
BACKGROUND
Lobed kudzuvine root and rhizoma chuanxiong are effective drugs in traditional Chinese medicine.


OBJECTIVE
Extracts of the two medicines were investigated for their in vitro of beta-amyloid (Aβ1-42)-aggregation-and acetylcholinesterase (AChE)-inhibitory activities.


METHODS
The interaction of lobed kudzuvine root, rhizoma chuanxiong with both acetylcholinesterase and beta-amyloid (Aβ1-42) were studied by Michaelis-Menten equations, Thioflavin T (ThT) fluorescence analysis and transmission electron microscope (TEM).


RESULTS
Inhibition of acetylcholinesterase showed that 1-butanol fraction of the two medicines were noncompetitive inhibition, apparent inhibition constants were 9.947 and 7.1523. ThT fluorescence analysis and TEM results indicated that inhibition of the water fraction and 1-butanol fraction (both lobed kudzuvine root and rhizoma chuanxiong) was better.


CONCLUSIONS
The result supported further research on chemical constituents and pharmacological mechanisms.
College of Pharmacy, South-Central University for Nationalities, Minyuan Road 708, Hongshan District, Wuhan, Hubei, 430074, China.
Li
L
Shuai
Zhi
Z
Chen
Ruixi
R
Gao
Tianming
T
Yang
Acetylcholinesterase; Beta-amyloid; lobed kudzuvine root; rhizoma chuanxiong