Site-specific immobilization of biomolecules by a biocompatible reaction between terminal cysteine and 2-cyanobenzothiazole.
We report herein a new site-specific microarray immobilization method based on a biocompatible reaction between terminal cysteine and 2-cyanobenzothiazole (CBT). This immobilization strategy has been successfully applied to anchor small molecules, peptides and proteins onto microarrays.
Department of Biology and Chemistry, City University of Hong Kong, 83 Tat Chee Avenue, Kowloon, Hong Kong, P. R. China. hongysun@cityu.edu.hk.
Ping
P
Wang
Chong-Jing
CJ
Zhang
Ganchao
G
Chen
Zhenkun
Z
Na
Shao Q
SQ
Yao
Hongyan
H
Sun
D000818:Animals; D052160:Benzothiazoles / Q000737:chemistry*; D002448:Cell Adhesion; D003545:Cysteine / Q000737:chemistry*; D055767:Immobilized Proteins / Q000737:chemistry*; D051379:Mice; D008958:Models, Molecular; D041681:NIH 3T3 Cells; D009570:Nitriles / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry*; D040081:Protein Array Analysis; D011506:Proteins / Q000737:chemistry*; D054852:Small Molecule Libraries / Q000737:chemistry*
C578355:2-cyanobenzothiazole; D052160:Benzothiazoles; D055767:Immobilized Proteins; D009570:Nitriles; D010455:Peptides; D011506:Proteins; D054852:Small Molecule Libraries; D003545:Cysteine