Downregulation of CPPED1 expression improves glucose metabolism in vitro in adipocytes.
We have previously demonstrated that the expression of calcineurin-like phosphoesterase domain containing 1 (CPPED1) decreases in adipose tissue (AT) after weight reduction. However, the function of CPPED1 in AT is unknown. Therefore, we investigated whether the change in CPPED1 expression is connected to changes in adipocyte glucose metabolism. First, we confirmed that the expression of CPPED1 decreased after weight loss in subcutaneous AT. Second, the expression of CPPED1 did not change during adipocyte differentiation. Third, CPPED1 knockdown with small interfering RNA increased expression of genes involved in glucose metabolism (adiponectin, adiponectin receptor 1, and GLUT4) and improved insulin-stimulated glucose uptake. To conclude, CPPED1 is a novel molecule involved in AT biology, and CPPED1 is involved in glucose uptake in adipocytes.
Institute of Public Health and Clinical Nutrition, Department of Clinical Nutrition, University of Eastern Finland, Kuopio, Finland.
Maija
M
Vaittinen
Dorota
D
Kaminska
Pirjo
P
Käkelä
Matti
M
Eskelinen
Marjukka
M
Kolehmainen
Jussi
J
Pihlajamäki
Matti
M
Uusitupa
Leena
L
Pulkkinen
D017667:Adipocytes / Q000378:metabolism*; D000273:Adipose Tissue / Q000378:metabolism; D000328:Adult; D000368:Aged; D019703:Calcineurin / Q000096:biosynthesis / Q000235:genetics*; D002454:Cell Differentiation / Q000502:physiology; D002478:Cells, Cultured; D015536:Down-Regulation; D055785:Gene Knockdown Techniques; D005947:Glucose / Q000378:metabolism*; D006801:Humans; D007328:Insulin / Q000494:pharmacology; D008875:Middle Aged; D034741:RNA, Small Interfering / Q000494:pharmacology; D015431:Weight Loss / Q000502:physiology*
D007328:Insulin; D034741:RNA, Small Interfering; C585177:CPPED1 protein, human; D019703:Calcineurin; D005947:Glucose