Deoxyribozyme-loaded nano-graphene oxide for simultaneous sensing and silencing of the hepatitis C virus gene in liver cells.
The multifunctional DNAzyme (Dz) delivery system is developed based on nano-sized graphene oxide (nGO) for simultaneous detection and knockdown of the target gene. The Dz/nGO complex system allowed convenient monitoring of HCV mRNA in living cells and silencing of the HCV gene expression by Dz-mediated catalytic cleavage concurrently.
Center for RNA Research, Institute for Basic Science, Department of Chemistry, Seoul National University, Seoul, 151-747, Republic of Korea.
Seongchan
S
Kim
Soo-Ryoon
SR
Ryoo
Hee-Kyung
HK
Na
Young-Kwan
YK
Kim
Byong-Seok
BS
Choi
Younghoon
Y
Lee
Dong-Eun
DE
Kim
Dal-Hee
DH
Min
D000998:Antiviral Agents / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D055162:Biocatalysis; D021881:DNA, Catalytic / Q000737:chemistry / Q000378:metabolism*; D015967:Gene Expression Regulation, Viral / Q000187:drug effects; D020868:Gene Silencing / Q000187:drug effects; D018014:Gene Transfer Techniques; D006108:Graphite / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D016174:Hepacivirus / Q000187:drug effects / Q000235:genetics*; D006801:Humans; D008099:Liver / Q000378:metabolism / Q000473:pathology / Q000821:virology; D008826:Microbial Sensitivity Tests; D049329:Nanostructures / Q000737:chemistry*; D010087:Oxides / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D010316:Particle Size; D012333:RNA, Messenger / Q000187:drug effects / Q000235:genetics; D013329:Structure-Activity Relationship; D013499:Surface Properties
D000998:Antiviral Agents; D021881:DNA, Catalytic; D010087:Oxides; D012333:RNA, Messenger; D006108:Graphite