In vivo evaluation of chitosan as an adjuvant in subcutaneous vaccine formulations.
Vaccines utilising pure antigens instead of whole pathogens and alternative administration routes require the use of potent adjuvants and effective antigen delivery systems. Chitosan has been reported to act as both an adjuvant as well as a matrix for delivery systems. Chitosan is a natural product produced predominantly from crab shell and commercially available preparations vary in molecular weight, degree of deacetylation and purity. In this study, the impact of chitosan characteristics (molecular weight, degree of deacetylation, particle size, viscosity and impurities) on adjuvant activity were examined. It could be shown that the degree of immune response differed if different chitosan qualities were used and this could be attributed to different characteristics of the chitosan qualities: the immunoadjuvant effect of chitosan probably is a result of an interplay between chemical properties such as molecular weight and degree of deacetylation and physical properties such as particle size and preparation technique, which impacts characteristics such as solubility and viscosity. Hence, the chitosan quality to be used as adjuvant in vaccine preparations needs to be selected carefully.
Department of Pharmaceutics and Biopharmaceutics, Christian Albrecht University, Grasweg 9a, 24118 Kiel, Germany. Electronic address: rscherliess@pharmazie.uni-kiel.de.
Regina
R
Scherließ
Simon
S
Buske
Katherine
K
Young
Benjamin
B
Weber
Thomas
T
Rades
Sarah
S
Hook
D000107:Acetylation; D000276:Adjuvants, Immunologic / Q000008:administration & dosage*; D000818:Animals; D048271:Chitosan / Q000008:administration & dosage* / Q000737:chemistry / Q000378:metabolism; D007279:Injections, Subcutaneous; D051379:Mice; D008810:Mice, Inbred C57BL; D008970:Molecular Weight; D014612:Vaccines / Q000008:administration & dosage* / Q000276:immunology*; D014783:Viscosity
D000276:Adjuvants, Immunologic; D014612:Vaccines; D048271:Chitosan
C57Bl/6 mice; Contaminants; Degree of deacetylation; Local depot; Molecular weight; Ovalbumin; Viscosity