[Study on HPLC chromatograms of different processed Euphorbia ebracteolata products and content change of three chemical components].
To prepare processed products with different methods, in order to study the impact of auxiliary materials and temperature on chemical components of Euphorbia ebracteolata, and establish specific chromatograms of different processed products. Wel-chorm-C18 column (4.6 mm x 250 mm, 5 microm) was used and eluted with a gradient program, with acetonitrile (A)-water(B). The column temperature was 25 degrees C, and the detection wave length was set at 226 nm. The aim was to determine the content of effective components in different processed products--ebracteolata cpd B, ebracteolata cpd C and jolkinolide B and establish respective characteristic fingerprints to compare with similarity. The results showed that the content of ebracteolata cpd B, ebracteolata cpd C first increased and then decreased with the rise in temperature. Different processed products showed significant difference in HPLC spectrograms, with a low similarity. This study showed great impacts of auxiliary materials and temperature on chemical components of E. ebracteolata. As the vinegar processing method had higher attenuation and and synergistic effects than other methods, the auxiliary material vinegar cannot be replaced by chemical reagent acetic acid.
Nanjing University of Traditional Chinese Medicine, Jiangsu Key Laboratory of Chinese Medicine Processing, Nanjing 210046, China.
Guo
G
Zhuang
Jun-Song
JS
Li
Bao-Chang
BC
Cai
D000098:Acetophenones / Q000032:analysis*; D002626:Chemistry, Pharmaceutical / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D004224:Diterpenes / Q000032:analysis*; D004365:Drugs, Chinese Herbal / Q000032:analysis*; D028481:Euphorbia / Q000737:chemistry*
C556516:4-dihydroxy-6-methoxy-3-methylacetophenone; D000098:Acetophenones; D004224:Diterpenes; D004365:Drugs, Chinese Herbal; C057914:jolkinolide B