Boron-doped nanocrystalline diamond microelectrode arrays monitor cardiac action potentials.
The expansion of diamond-based electronics in the area of biological interfacing has not been as thoroughly explored as applications in electrochemical sensing. However, the biocompatibility of diamond, large safe electrochemical window, stability, and tunable electronic properties provide opportunities to develop new devices for interfacing with electrogenic cells. Here, the fabrication of microelectrode arrays (MEAs) with boron-doped nanocrystalline diamond (BNCD) electrodes and their interfacing with cardiomyocyte-like HL-1 cells to detect cardiac action potentials are presented. A nonreductive means of structuring doped and undoped diamond on the same substrate is shown. The resulting BNCD electrodes show high stability under mechanical stress generated by the cells. It is shown that by fabricating the entire surface of the MEA with NCD, in patterns of conductive doped, and isolating undoped regions, signal detection may be improved up to four-fold over BNCD electrodes passivated with traditional isolators.
ICS/PGI-8 Bioelectronics, Forschungszentrum Jülich GmbH, Jülich, 52425, Germany.
Vanessa
V
Maybeck
Robert
R
Edgington
Alexandre
A
Bongrain
Joseph O
JO
Welch
Emanuel
E
Scorsone
Philippe
P
Bergonzo
Richard B
RB
Jackman
Andreas
A
Offenhäusser
D000200:Action Potentials / Q000502:physiology*; D001895:Boron / Q000737:chemistry*; D002460:Cell Line; D018130:Diamond / Q000737:chemistry*; D006801:Humans; D008839:Microelectrodes
D018130:Diamond; D001895:Boron
action potential; diamond microelectrode array; nanocrystalline diamond