[Study on preparation of quercetin nanostructured lipid carriers and their physicochemical properties].
OBJECTIVE
To prepare quercetin nanostuctured lipid carriers (QT-NLC), and detect their physicochemical properties.


METHODS
QT-NLC was prepared by emulsification ultrasonic dispersion method, and the optimum prescription was screened out by orthogonal design. Transmission electron microscope was used to observe QT-NLC morphology. Granulometer was applied to determine zeta potential, particle size and distribution. DSC was adopted for phase analysis. Centrifugal ultra-filtration method was used to determine entrapment efficiency. Dialysis method was adopted to detect drug release in vitro of preparations.


RESULTS
QT-NLC prepared under optimum conditions was mostly spherical grains, with the average particle size of (175 +/- 25) nm, which were distributed evenly, and zeta potential was (-23 +/- 0.3) mV. DSC results indicated that the drug was dispersed in nano-particles in a non-crystalline state, with an entrapment efficiency of (95.43 +/- 0.23)% and a drug-loading capacity of (2.38 +/- 0.24)%. The in vitro drug release was 32.2% in 2 hours, which was followed by a sustained release.


CONCLUSIONS
Emulsification ultrasonic dispersion method is applicable for preparing QT-NLC, as nano-particles are distributed evenly, with good reliability. This processing technology is safe, reliable and highly reproducible.
Key Laboratory of Biological Formulations of Colleges in Heilongjiang Province, School of Pharmacy, Jiamusi University, Jiamusi 154007, China. jdyulian@163.com
Lian
L
Yu
Jin-Ru
JR
Yang
Yang
Y
Liu
Tong
T
Zhou
Yu-Ming
YM
Kuang
Shan-Shan
SS
Lu
Teng-Yuan
TY
Zhang
Yu
Y
Guo
D003692:Delayed-Action Preparations; D004337:Drug Carriers; D004655:Emulsions; D008055:Lipids / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size; D011794:Quercetin / Q000737:chemistry*
D003692:Delayed-Action Preparations; D004337:Drug Carriers; D004655:Emulsions; D008055:Lipids; D011794:Quercetin