[DNA extraction from bones and teeth using AutoMate Express forensic DNA extraction system].
OBJECTIVE
To explore a new method in order to extract DNA from bones and teeth automatically.


METHODS
Samples of 33 bones and 15 teeth were acquired by freeze-mill method and manual method, respectively. DNA materials were extracted and quantified from the triturated samples by AutoMate Express forensic DNA extraction system.


RESULTS
DNA extraction from bones and teeth were completed in 3 hours using the AutoMate Express forensic DNA extraction system. There was no statistical difference between the two methods in the DNA concentration of bones. Both bones and teeth got the good STR typing by freeze-mill method, and the DNA concentration of teeth was higher than those by manual method.


CONCLUSIONS
AutoMate Express forensic DNA extraction system is a new method to extract DNA from bones and teeth, which can be applied in forensic practice.
Institute of Criminal Science and Technology, Hangzhou Public Security Bureau, Hangzhou 310004, China. myhappyfamily_gao@163.com
Lin-Lin
LL
Gao
Nian-Lai
NL
Xu
Wei
W
Xie
Shao-Cheng
SC
Ding
Dong-Jing
DJ
Wang
Li-Qin
LQ
Ma
You-Ying
YY
Li
D001331:Automation; D001842:Bone and Bones / Q000737:chemistry*; D004247:DNA / Q000302:isolation & purification*; D016172:DNA Fingerprinting / Q000295:instrumentation / Q000379:methods*; D005554:Forensic Medicine / Q000379:methods*; D006801:Humans; D018895:Microsatellite Repeats; D016133:Polymerase Chain Reaction; D013048:Specimen Handling / Q000379:methods; D014070:Tooth / Q000737:chemistry*
D004247:DNA