Effect of newly isolated Lactobacillus ingluviei ADK10, from chicken intestinal tract on acetaminophen induced oxidative stress in Wistar rats.
The total antioxidative activity of L. ingluviei ADK10 isolated from chicken intestine intact cells and cell free culture supernatant (CFCS) was 54- 67.95%. The ability to scavenge a,a-Diphenyl-b-Picrylhydrazyl free radical ranged from 71 and 64% in intact cells and CFCS respectively. Total reducing activity of bacteria was equivalent to 290 microM/L of cysteine. Reducing glutathione activity was equivalent to 93.95 microg/mL. Oral administration of the strain at a dose of 10(9) cfu/kg body weight to acetaminophen induced oxidative stress in rats increased catalase, glutathione and superoxide dismutase activity in the blood, liver and kidney and lowered malondialdehyde level. The results indicate that L. ingluviei ADK10 has potential free radical scavenging activity for the treatment of oxidative stress related disease.
Department of Microbiology, Nutrition, and Human Physiology, Raja N L Khans Women's College, Midnapore 721 102, India.
Arpita
A
Mandal
Tanmay
T
Paul
Suchismita
S
Roy
Shreya
S
Mandal
Shrabani
S
Pradhan
Keshab Ch
KCh
Mondal
Dilip Kumar
DK
Nandi
D000082:Acetaminophen; D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D002374:Catalase / Q000097:blood; D002645:Chickens / Q000382:microbiology*; D005978:Glutathione / Q000097:blood; D007422:Intestines / Q000382:microbiology*; D007668:Kidney / Q000201:enzymology; D007778:Lactobacillus / Q000145:classification / Q000302:isolation & purification* / Q000378:metabolism*; D008099:Liver / Q000201:enzymology / Q000473:pathology; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D010802:Phylogeny; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000097:blood
D000975:Antioxidants; D000082:Acetaminophen; D008315:Malondialdehyde; D002374:Catalase; D013482:Superoxide Dismutase; D005978:Glutathione