Monochloramine suppresses the proliferation of colorectal cancer cell line Caco-2 by both apoptosis and G2/M cell cycle arrest.
The aim of this study was to assess a possible role of monochloramine (NH2 Cl), one of the reactive chlorine species, which induce oxidative stress, on the proliferation of colorectal cancer cell line Caco-2. At concentrations ranging from 10 to 200 μM, NH2 Cl (14-61% inhibition), but not hypochlorous acid, dose-dependently inhibited the cell viability of Caco-2 cells. Experiments utilizing methionine (a scavenger of NH2 Cl), taurine-chloramine and glutamine-chloramine revealed that only NH2 Cl affects the cancer cell proliferation among reactive chlorine species, with a relative specificity. Furthermore, flow-cytometry experiments showed that the anti-proliferative effect of NH2 Cl is partially attributable to both apoptosis and G2/M cell cycle arrest. These results suggest that NH2 Cl has the potential to suppress colorectal cancer cell proliferation.
Laboratory of Physiological Chemistry, Osaka University of Pharmaceutical Sciences, Takatsuki, Osaka, Japan.
Tetsuya
T
Kohda
Satoru
S
Sakuma
Muneyuki
M
Abe
Yohko
Y
Fujimoto
D017209:Apoptosis / Q000187:drug effects*; D018938:Caco-2 Cells; D049109:Cell Proliferation / Q000187:drug effects*; D002700:Chloramines / Q000378:metabolism / Q000494:pharmacology*; D015179:Colorectal Neoplasms / Q000473:pathology*; D059565:G2 Phase Cell Cycle Checkpoints / Q000187:drug effects*; D006801:Humans
D002700:Chloramines; C030816:chloramine
colon cancer; monochloramine; oxidative stress; proliferation; reactive chlorine species