TGFβ-pathway is down-regulated in a uterine carcinosarcoma: a case study.
Data assessing the role of various genetic alterations in uterine carcinosarcoma (CS), particularly the transforming growth factors-β (TGFβ) that play a crucial role in many cellular processes, including proliferation, differentiation, adhesion and migration, are scarce. TGFβ exert their effects through specific receptors and associated auxiliary receptors. In the current study, we investigated the expression of TGFβ isoforms and their receptors, as well as selected genes in a case of CS. We applied the real-time fluorescence detection PCR method with FAM dye-labeled TaqMan specific probes. In a comparison to the normal counterpart, TGFB1, TGFB2, TGFBRII, TGFBR3, ENG and CD109 were all down-regulated in uterine CS samples at different extents. BIRC5 and hTERT, markers of tumor survival, were up-regulated in CS as compared with normal counterparts. A concomitant increase of the hypoxia marker HIF1A expression pattern was noted, whereas the expression of GPR120, responsible for free fatty acids sensing, was not different in both counterparts evaluated. In conclusion, deregulation of various cellular mechanisms in uterine CS is associated with alterations at many levels - cell growth and proliferation, apoptosis, and impaired response to stimuli from extracellular environment.
IInd Department of Gynecology, Lublin Medical University, Lublin, Poland. Electronic address: andrzej.semczuk@am.lublin.pl.
Andrzej
A
Semczuk
Piotr K
PK
Zakrzewski
Ewa
E
Forma
Adam I
AI
Cygankiewicz
Anna
A
Semczuk-Sikora
Magdalena
M
Bryś
Tomasz
T
Rechberger
Wanda M
WM
Krajewska
D000368:Aged; D017209:Apoptosis / Q000235:genetics; D002296:Carcinosarcoma / Q000235:genetics* / Q000473:pathology; D049109:Cell Proliferation; D015536:Down-Regulation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D012333:RNA, Messenger / Q000032:analysis; D015398:Signal Transduction / Q000235:genetics*; D016212:Transforming Growth Factor beta / Q000235:genetics*; D014594:Uterine Neoplasms / Q000235:genetics* / Q000473:pathology
D012333:RNA, Messenger; D016212:Transforming Growth Factor beta
CS; DγC; EC; LVSI; RT-PCR; TGFβ; Uterine carcinosarcoma; carcinosarcoma; dilatation and curettage; endometrial cancer; lymphovascular space invasion