Rapid Detection of Bovine Respiratory Syncytial Virus in Poland Using a Human Patient-Side Diagnostic Assay.
Bovine respiratory syncytial virus (BRSV) plays a significant role in the etiopathogenesis of the respiratory syndrome in young cattle during their first year of life. Development of rapid and accurate BRSV diagnostic tools would aid in the appropriate control of this important pathogen. The objective of this study was to characterize infections induced by BRSV by means of rapid patient-side immunomigration assays used for diagnosis of human respiratory syncytial virus (hRSV) in humans. Nasal and tracheal swabs were obtained from healthy calves of various beef and dairy breeds - Holstein-Friesian, Simmental, Charolais, Belgian Blue and Limousin, between the ages of 5 and 12 months, from 26 farms. BRSV was identified using two rapid immunomigration assays, TruRSV® and Clearview® RSV, and compared with RT-PCR as a reference technique. BRSV was found in 73.1% of all the herds tested. High agreement with RT-PCR was obtained for TruRSV® (κ = 0.824), while in the case of the Clearview® RSV test, agreement with PCR was moderate (κ = 0.420). The results demonstrate that rapid patient-side immunomigration assays designed to detect hRSV can be used to accurately detect BRSV in field samples collected from cattle.
Sub-department of Veterinary Prevention, Institute of Biological Bases of Animal Diseases, Faculty of Veterinary Medicine, University of Life Sciences, Lublin, Poland.
R
R
Urban-Chmiel
Sub-department of Veterinary Prevention, Institute of Biological Bases of Animal Diseases, Faculty of Veterinary Medicine, University of Life Sciences, Lublin, Poland.
A
A
Wernicki
Department of Large Animal Clinical Sciences, College of Veterinary Medicine, Michigan State University, East Lansing, MI, USA.
D L
DL
Grooms
Department of Large Animal Clinical Sciences, College of Veterinary Medicine, Michigan State University, East Lansing, MI, USA.
N I
NI
Barbu
Department of Virology, National Veterinary Research Institute, Pulawy, Poland.
J
J
Rola
Department of Virology, National Veterinary Research Institute, Pulawy, Poland.
W
W
Socha
D000818:Animals; D000956:Antigens, Viral / Q000145:classification / Q000302:isolation & purification; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis* / Q000276:immunology / Q000821:virology; D007118:Immunoassay / Q000379:methods*; D009297:Nasal Mucosa / Q000821:virology; D011044:Poland; D016133:Polymerase Chain Reaction; D011933:Reagent Kits, Diagnostic; D018357:Respiratory Syncytial Virus Infections / Q000175:diagnosis / Q000276:immunology / Q000662:veterinary*; D018112:Respiratory Syncytial Virus, Bovine / Q000276:immunology / Q000302:isolation & purification*; D014132:Trachea / Q000821:virology
D000956:Antigens, Viral; D011933:Reagent Kits, Diagnostic
BRSV; calves; strip tests