Aging studies in Drosophila melanogaster.
Drosophila is a genetically tractable system ideal for investigating the mechanisms of aging and developing interventions for promoting healthy aging. Here we describe methods commonly used in Drosophila aging research. These include basic approaches for preparation of diets and measurements of lifespan, food intake, and reproductive output. We also describe some commonly used assays to measure changes in physiological and behavioral functions of Drosophila in aging, such as stress resistance and locomotor activity.
Translational Gerontology Branch, National Institute on Aging, Baltimore, MD, USA.
Yaning
Y
Sun
Jason
J
Yolitz
Cecilia
C
Wang
Edward
E
Spangler
Ming
M
Zhan
Sige
S
Zou
D000375:Aging / Q000502:physiology*; D000818:Animals; D031204:Caloric Restriction; D004032:Diet; D029721:Drosophila Proteins / Q000235:genetics; D004331:Drosophila melanogaster / Q000235:genetics* / Q000502:physiology*; D004435:Eating; D008136:Longevity / Q000235:genetics*; D009043:Motor Activity / Q000502:physiology; D018384:Oxidative Stress / Q000502:physiology; D043562:Receptors, G-Protein-Coupled / Q000235:genetics; D012098:Reproduction; D013312:Stress, Physiological / Q000502:physiology
D029721:Drosophila Proteins; D043562:Receptors, G-Protein-Coupled; C115381:mth protein, Drosophila