[Observations of in vitro pollen germination of Prunella vulgaris].
To determine the optimal condition of pollen germination. The pollen of Prunella vulgaris was cultured in vitro. Pollen germination rates were recorded using 10% H3BO4, 30% Ca(NO3)2, 20% MgSO4 and 10% KNO3 as the basic mineral medium with PEG of different molecular weight, sucrose of various density and multiple pH value. The rates were also measured under different cultivation temperature and pollen acquisition time. The optimal condition of pollen germination is 10% H3 BO4, 30% Ca(NO3)2, 20% MgSO4, 10% KNO3, and 25% PEG-4000 as the medium, with pH about 6. 5 and pollen acquired at the beginning of blossom.
Institute of Chinese Medicinal, Nanjing Agricultural University, Nanjing 210095, China.
Yi-Min
YM
Li
De-Qian
DQ
Wan
Qiao-Sheng
QS
Guo
Yue-Sheng
YS
Xie
Man
M
Zhou
D035264:Flowers / Q000502:physiology; D011058:Pollen / Q000502:physiology*; D031347:Prunella / Q000502:physiology*