Immobilization of whole cells by chemical vapor deposition of silica.
Effective entrapment of whole bacterial cells onto solid-phase materials can significantly improve bioprocessing and other biotechnology applications. Cell immobilization allows integration of biocatalysts in a manner that maintains long-term cell viability and typically enhances process output. A wide variety of functionalized materials have been explored for microbial cell immobilization, and specific advantages and limitations were identified. The method described here is a simple, versatile, and scalable one-step process for the chemical vapor deposition of silica to encapsulate and stabilize viable, whole bacterial cells. The immobilized bacterial population is prepared and captured at a predefined physiological state so as to affix bacteria with a selected metabolic or catalytic capability to compatible materials and surfaces. Immobilization of Shewanella oneidensis to carbon electrodes and immobilization of Acinetobacter venetianus to adsorbent mats are described as model systems.
Universal Technology Corporation, Dayton, OH, USA.
Susan R
SR
Sizemore
Robert
R
Nichols
Randi
R
Tatum
Plamen
P
Atanassov
Glenn R
GR
Johnson
Heather R
HR
Luckarift
D000150:Acinetobacter / Q000166:cytology / Q000502:physiology; D000255:Adenosine Triphosphate / Q000096:biosynthesis; D000327:Adsorption; D055162:Biocatalysis; D018441:Biofilms; D018914:Cells, Immobilized / Q000737:chemistry; D004566:Electrodes; D006108:Graphite / Q000737:chemistry; D050296:Microbial Viability; D020592:Shewanella / Q000166:cytology / Q000502:physiology; D012822:Silicon Dioxide / Q000737:chemistry*; D014835:Volatilization
D012822:Silicon Dioxide; D006108:Graphite; D000255:Adenosine Triphosphate