Hydrogen/deuterium exchange mass spectrometry for probing higher order structure of protein therapeutics: methodology and applications.
The higher order structure of protein therapeutics can be interrogated with hydrogen/deuterium exchange mass spectrometry (HDX-MS). HDX-MS is now a widely used tool in the structural characterization of protein therapeutics. In this review, HDX-MS based workflows designed for protein therapeutic discovery and development processes are presented, focusing on the specific applications of epitope mapping for protein/drug interactions and biopharmaceutical comparability studies. Future trends in the application of HDX-MS in protein therapeutics characterization are also described.
Bioanalytical and Discovery Analytical Sciences, Research and Development, Bristol-Myers Squibb, Princeton, NJ, USA.
Hui
H
Wei
Bioanalytical and Discovery Analytical Sciences, Research and Development, Bristol-Myers Squibb, Princeton, NJ, USA.
Jingjie
J
Mo
Biologics Manufacturing and Process Development, Global Manufacturing and Supply, Bristol-Myers Squibb, Hopewell, NJ, USA.
Li
L
Tao
Biologics Manufacturing and Process Development, Global Manufacturing and Supply, Bristol-Myers Squibb, Bloomsbury, NJ, USA.
Reb J
RJ
Russell
Bioanalytical and Discovery Analytical Sciences, Research and Development, Bristol-Myers Squibb, Princeton, NJ, USA.
Adrienne A
AA
Tymiak
Bioanalytical and Discovery Analytical Sciences, Research and Development, Bristol-Myers Squibb, Princeton, NJ, USA. Electronic address: Guodong.Chen@bms.com.
Guodong
G
Chen
Department of Chemistry & Chemical Biology, Northeastern University, Boston, MA, USA.
Roxana E
RE
Iacob
Department of Chemistry & Chemical Biology, Northeastern University, Boston, MA, USA. Electronic address: J.Engen@neu.edu.
John R
JR
Engen
D000818:Animals; D003903:Deuterium / Q000737:chemistry*; D006801:Humans; D006859:Hydrogen / Q000737:chemistry*; D013058:Mass Spectrometry / Q000379:methods*; D011485:Protein Binding / Q000502:physiology; D060066:Protein Interaction Maps / Q000502:physiology*; D017433:Protein Structure, Secondary; D021381:Protein Transport / Q000502:physiology
D006859:Hydrogen; D003903:Deuterium