Highly efficient biotransformation of polydatin to resveratrol by snailase hydrolysis using response surface methodology optimization.
Resveratrol (RV), a dietary antioxidant polyphenolic compound found in grapes and red wine, exerts a wide variety of pharmacological activities. However, lower content in plants compared with polydatin (PD, the glucoside of RV) limits its application in the food and pharmaceutical industries. In this paper, we carried out efficient biotransformation of PD to RV with 100% conversion yield by snailase hydrolysis. Moreover, response surface methodology (RSM) was used to optimize the effects of the reaction temperature, enzyme load, and reaction time on the conversion process. Validation of the RSM model was verified by the good agreement between the experimental and the predicted RV yield values. The optimum preparation conditions were as follows: temperature of 62.0 °C, enzyme load of 6.6%, and reaction time of 96 min. The proposed method may be highly applicable for the enzymatic preparation of RV for medicinal purposes.
College of Chinese Medicinal Materials, Jilin Agricultural University, Changchun 130118, China.
Zi
Z
Wang
Li-Chun
LC
Zhao
Wei
W
Li
Lian-Xue
LX
Zhang
Jing
J
Zhang
Jian
J
Liang
D000975:Antioxidants / Q000737:chemistry; D001711:Biotransformation; D005960:Glucosides / Q000737:chemistry*; D006801:Humans; D006868:Hydrolysis; D000077185:Resveratrol; D013267:Stilbenes / Q000737:chemistry*; D013499:Surface Properties; D013696:Temperature; D027843:Vitis / Q000737:chemistry
D000975:Antioxidants; D005960:Glucosides; D013267:Stilbenes; D000077185:Resveratrol; C058229:polydatin