A constrained tricyclic nucleic acid analogue of α-L-LNA: investigating the effects of dual conformational restriction on duplex thermal stability.
A constrained tricyclic analogue of α-L-LNA (2), which contains dual modes of conformational restriction about the ribose sugar moiety, has been synthesized and characterized by X-ray crystallography. Thermal denaturation experiments of oligonucleotide sequences containing this tricyclic α-L-LNA analogue (α-L-TriNA 2, 5) indicate that this modification is moderately stabilizing when paired with complementary DNA and RNA, but less stabilizing than both α-L-LNA (2) and α-L-TriNA 1 (4).
Department of Chemistry, Université de Montréal , Montréal, QC, H3C 3J7, Canada.
Stephen
S
Hanessian
Jernej
J
Wagger
Bradley L
BL
Merner
Robert D
RD
Giacometti
Michael E
ME
Ostergaard
Eric E
EE
Swayze
Punit P
PP
Seth
D018360:Crystallography, X-Ray; D008958:Models, Molecular; D008968:Molecular Conformation; D009691:Nucleic Acid Denaturation; D009841:Oligonucleotides / Q000138:chemical synthesis / Q000737:chemistry*; D013696:Temperature
D009841:Oligonucleotides; C477371:locked nucleic acid