Isoflavonoid-Rich Flemingia macrophylla Extract Attenuates UVB-Induced Skin Damage by Scavenging Reactive Oxygen Species and Inhibiting MAP Kinase and MMP Expression.
In this study, we investigated the antioxidant activity and anti-photoaging properties of an extract of Flemingia macrophylla, a plant rich in isoflavonoid content. Pretreatment of fibroblasts with Flemingia macrophylla extract (FME) inhibited elastase activity, promoted the protein expression of type I procollagen, and attenuated the phosphorylation of mitogen-activated protein (MAP) kinase and the protein expression of matrix-metalloproteinase- (MMP-) 1, 3, and 9. The IC50 values were 2.1  μ g/mL for DPPH radical scavenging ability, 366.8  μ g/mL for superoxide anion scavenging ability, 178.9  μ g/mL for hydrogen peroxide scavenging ability, and 230.9  μ g/mL for hydroxyl radical scavenging ability. Also, exposure of erythrocytes to various concentrations of FME (50-500  μ g/mL) resulted in a dose- and time-dependent inhibition of AAPH-induced hemolysis. In human fibroblasts, FME at 10  μ g/mL was shown to be a potent scavenger of UV-induced reactive oxygen species (ROS). The antioxidant and anti-photoaging properties of FME make it an ideal anti-intrinsic aging and anti-photoaging agent.
Department of Cosmeceutics, China Medical University, Taichung 404, Taiwan.
Hsiu-Mei
HM
Chiang
Hua-Hsien
HH
Chiu
Sue-Tsai
ST
Liao
Yen-Ting
YT
Chen
Hsien-Chang
HC
Chang
Kuo-Ching
KC
Wen