Specific RNA-protein interactions detected with saturation transfer difference NMR.
RNA, at the forefront of biochemical research due to its central role in biology, is recognized by proteins through various mechanisms. Analysis of the RNA-protein interface provides insight into the recognition determinants and function. As such, there is a demand for developing new methods to characterize RNA-protein interactions. Saturation transfer difference (STD) NMR can identify binding ligands for proteins in a rather short period of time, with data acquisitions of just a few hours. Two RNA-protein systems involved in RNA modification were studied using STD NMR. The N (6)-threonylcarbamoyltransferase, YrdC, with nucleoside-specific recognition, was shown to bind the anticodon stem-loop of tRNA(Lys)UUU. The points of contact on the RNA were assigned and a binding interface was identified. STD NMR was also applied to the interaction of the archaeal ribosomal protein, L7Ae, with the box C/D K-turn RNA. The distinctiveness of the two RNA-protein interfaces was evident. Both RNAs exhibited strong STD signals indicative of direct contact with the respective protein, but reflected the nature of recognition. Characterization of nucleic acid recognition determinants traditionally involves cost and time prohibitive methods. This approach offers significant insight into interaction interfaces fairly rapidly, and complements existing structural methods.
The RNA Institute; University at Albany; Albany, NY USA; Department of Biological Sciences; University at Albany; Albany, NY USA.
Kimberly A
KA
Harris
Alexander
A
Shekhtman
Paul F
PF
Agris
D000926:Anticodon; D001105:Archaea / Q000378:metabolism; D001665:Binding Sites; D002942:Circular Dichroism; D029968:Escherichia coli Proteins / Q000378:metabolism*; D008958:Models, Molecular; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D009690:Nucleic Acid Conformation; D011485:Protein Binding; D011506:Proteins / Q000378:metabolism*; D012313:RNA / Q000235:genetics / Q000378:metabolism*; D012329:RNA, Bacterial / Q000737:chemistry / Q000378:metabolism; D012357:RNA, Transfer, Lys / Q000737:chemistry / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000378:metabolism*
D000926:Anticodon; D029968:Escherichia coli Proteins; D011506:Proteins; D012329:RNA, Bacterial; D012357:RNA, Transfer, Lys; D016601:RNA-Binding Proteins; C426646:YrdC protein, E coli; D012313:RNA
L7Ae; RNA modification; YrdC; lysyl-tRNA; modification enzymes; nuclear magnetic resonance; snoRNA