Isolation and sequence determination of tetrapeptide from Selaginella bryopteris.
A simple and inexpensive paper chromatographic method was developed for the isolation of a small peptide from Selaginella bryopteris fronds. The content of peptides is low in most plants and the isolation and purification procedure is troublesome. This method may be used as a first step for the detection of small peptides in the plant extracts. De novo sequence determination by tandem mass spectrometry indicated that the peptide is a tetrapeptide.
a Department of Biochemistry , PSG College of Arts and Science , Civil Aerodrome Post, Coimbatore Tamil Nadu 641014 , India.
M Rajeswari
MR
Prabha
B
B
Ramachandramurty
D002854:Chromatography, Paper; D007202:Indicators and Reagents / Q000138:chemical synthesis; D015394:Molecular Structure; D009842:Oligopeptides / Q000737:chemistry / Q000302:isolation & purification*; D010936:Plant Extracts / Q000737:chemistry; D032503:Selaginellaceae / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared
D007202:Indicators and Reagents; D009842:Oligopeptides; D010936:Plant Extracts