[Study on all-time multi-wavelength fusion fingerprint of Qizhiweitong granules and multi-component quantitative analysis].
OBJECTIVE
To control the quality of Qizhiweitong granules with the all-time multi-wavelength fusion fingerprint quantification as the major technique.


METHODS
Agilent TC-C18 (4.6 mm x 250 mm, 5 microm) chromatographic column was adopted, with 0.02% formic acid water-acetonitrile as the mobile phase for linear gradient elution. The flow rate was 1 mL x min(-1), column temperature was 30 degrees C, and detector wavelength was 230, 254, 283 nm. Matlab was adopted for all-time multiple-wavelength fusion for data in dif format.


RESULTS
A good relationship was shown for albiflorin in 56.5-452 mg x L(-1) (r = 0.999 8), paeoniflorin in 107-856 mg x L(-1) (r = 0.999 8), licorice glycoside in 73.4-687 mg x L(-1) (r = 0.999 8), naringin in 109-872 mg x L(-1) (r = 0.999 8), neohesperidin in 48.0-384 mg L(-1) (r = 0.999 8), and glycyrrhizic acid in 38.6-308 mg x L(-1) (r = 0.999 8), with recoveries of 0.999 8.


CONCLUSIONS
The method is simple, accurate and highly reproducible, and can provide basis for quality control of Qizhiweitong granules.
College of Pharmacy, Liaoning University of Traditional Chinese Medicine, Dalian 116600, China. yaodong_2010@163.com
Dong
D
Yao
Xian-Sheng
XS
Meng
Shuai
S
Wang
Yong-Rui
YR
Bao
Ying
Y
Pan
Ling
L
Han
D001565:Benzoates / Q000032:analysis; D001952:Bridged-Ring Compounds / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000032:analysis*; D005960:Glucosides / Q000032:analysis; D006027:Glycosides / Q000032:analysis; D039821:Monoterpenes; D011786:Quality Control
D001565:Benzoates; D001952:Bridged-Ring Compounds; D004365:Drugs, Chinese Herbal; D005960:Glucosides; D006027:Glycosides; D039821:Monoterpenes; C015423:peoniflorin; C014959:albiflorin