Nephroprotective effects of Isaria felina in rats with adenine-induced chronic renal failure.
OBJECTIVE
Chronic renal failure (CRF) is a progressive, life-threatening condition with limited treatment options. Cordyceps sinensis is a fungus that has nephroprotective effects, and Isaria felina (IF) is a fungus isolated from C. sinensis fruiting bodies. We evaluated IF efficacy using an adenine-induced CRF animal model.


METHODS
Forty male Sprague-Dawley rats were divided into normal control (n = 8) and adenine groups (n = 32; 100 mg/kg for 30 days). The adenine group was subdivided into a model control group (n = 7), a positive control group (200 mg/kg Jinshuibao capsule (JSB; n = 8), and two IF groups (200 mg/kg, n = 8; 100 mg/kg, n = 8). After treatment for 30 days, animals were narcotized and abdominal aortic blood was analysed. Kidney functions were evaluated.


RESULTS
Higher serum creatinine, blood urea nitrogen and uric acid levels, and lower creatinine clearance was observed in the model control group compared with JSB and IF groups (P < 0.05). Red blood cell count, haemoglobin and haematocrit levels in the 200 mg/kg IF group were higher than in the model control group (P < 0.05). Transforming growth factor-β1 mRNA expression in the model control group was higher than the normal control and 200 mg/kg IF groups (P < 0.05). Epidermal growth factor mRNA in the model control group was lower than in the normal control and both IF-treated groups (P < 0.05). Structural renal damage was observed in all adenine-treated rats, but was less severe in the JSB and IF groups.


CONCLUSIONS
IF may reverse the damaged kidney functions-induced with adenine in rats.
College of Chemistry and Chemical Engineering, Shanxi University, Taiyuan 030006, China.
Xihua
X
Yang
Shengwan
S
Zhang
Liansheng
L
Ren
Hong
H
Zhang
Xihua
X
Bai
D000225:Adenine; D000818:Animals; D001203:Ascomycota; D001688:Biological Products / Q000494:pharmacology / Q000627:therapeutic use*; D001806:Blood Urea Nitrogen; D032661:Cordyceps; D003404:Creatinine / Q000097:blood; D004195:Disease Models, Animal; D004815:Epidermal Growth Factor / Q000235:genetics / Q000378:metabolism; D004906:Erythrocyte Count; D048690:Fruiting Bodies, Fungal; D006400:Hematocrit; D006454:Hemoglobins / Q000378:metabolism; D007668:Kidney / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D007676:Kidney Failure, Chronic / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D008297:Male; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D053773:Transforming Growth Factor beta1 / Q000235:genetics / Q000378:metabolism; D014527:Uric Acid / Q000097:blood
D001688:Biological Products; D006454:Hemoglobins; D012333:RNA, Messenger; D053773:Transforming Growth Factor beta1; D014527:Uric Acid; D004815:Epidermal Growth Factor; D003404:Creatinine; D000225:Adenine
Isaria felina; adenine; chronic renal failure; rats