Enantioseparation of tartaric acid by ligand-exchange capillary electrophoresis using contactless conductivity detection.
A method for the determination of tartaric acid enantiomers using CE with contactless conductivity detection has been developed. Cu(II) as a central metal ion together with L-hydroxyproline were used as a chiral selector, the BGE was composed of 7 mM CuCl2, 14 mM trans-4-hydroxy-L-proline, and 100 mM ε-aminocaproic acid; the pH was adjusted to 5 by hydrochloric acid. Separation with a resolution of 1.9 was achieved in 9 min in a polyacrylamide-coated capillary to suppress the EOF. Various counterions of the BGE were studied, and migration order reversal was achieved when switching from ε-aminocaproic acid to L-histidine. With detection limits of about 20 μM, the method was applied to the analysis of wine and grape samples; only L-tartaric acid was found.
Department of Analytical Chemistry, Regional Centre of Advanced Technologies and Materials, Faculty of Science, Palacky University in Olomouc, Olomouc, Czech Republic.
Radim
R
Knob
Jan
J
Petr
Juraj
J
Sevčík
Vítězslav
V
Maier
D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods*; D010936:Plant Extracts / Q000737:chemistry*; D013237:Stereoisomerism; D013644:Tartrates / Q000737:chemistry*; D027843:Vitis / Q000737:chemistry*; D014920:Wine / Q000032:analysis*
D010936:Plant Extracts; D013644:Tartrates; C029768:tartaric acid
Capillary electrophoresis; Contactless conductivity detection; Enantioseparation; Ligand exchange; Tartaric acid