Mathematical model and dynamic computer simulation on free flow zone electrophoresis.
In this paper, a mathematical model was proposed for calculating physico-chemical parameters and simulating the separation process of solutes in free-flow zone electrophoresis (FFZE). Computer software was developed and implemented in a Delphi XE2 environment based on the model, which comprises zone electrophoresis, electrolyte solution, hydrodynamics and diffusion as well as conversion equations. The simulation results reveal that (i) the software is capable of simulating a dynamic process of FFZE properly; (ii) the software can simulate operation parameters (e.g., electric field, flow rate and pH value) for experimental optimization; (iii) the simulator is able to display the final electropherogram of numerous analytes in FFZE; and (iv) the electropherogram of FFZE can be automatically converted to that of capillary zone electrophoresis. In order to verify the rationality and reliability of this software, the relevant experiments were conducted and further compared with the simulations. The comparisons demonstrated the high agreement between the simulations and the experiments as well as those cited from the relevant references. In addition, effective mobility, diffusion coefficient and concentration of solutes can be conveniently gained via the software. The developed mathematical model and designed software have evident significance for the optimization of experimental conditions and basic physico-chemical parameters in FFZE.
Laboratory of Bioseparation and Analytical Biochemistry, State Key Laboratory of Microbial Metabolism, School of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China. cxcao@sjtu.edu.cn.
Jie
J
Zhang
Jian
J
Yan
Si
S
Li
Bo
B
Pang
Cheng-Gang
CG
Guo
Cheng-Xi
CX
Cao
Xin-Qiao
XQ
Jin
D000596:Amino Acids / Q000032:analysis / Q000737:chemistry; D003198:Computer Simulation; D019075:Electrophoresis, Capillary / Q000379:methods; D008962:Models, Theoretical
D000596:Amino Acids