[Optinization of rapid propagation technique and induction and identification of autotetraploid of Polygonum multiflorum].
OBJECTIVE
To establish and optimize the rapid propagation system of Polygonum multiflorum, as well as explore method for induction and identification of autotetraploid.


METHODS
Propagation medium was optimized by orthogonal test. The buds were immersed in colchicine solution with different concentrations for different time to select induction conditions for autotetraploid of P. multiflorum.


RESULTS
The most appropriate propagation medium was MS medium supplemented with 1.0 mg x L(-1) 6-BA, 0.3 mg x L(-1) NAA, and 0.4 mg x L(-1) PP333. That the buds were soaked in 0.2% colchicine solution for 30 h, or soaked in 0.3% colchicine solution for 18 h, was optimal condition to induce autopolyploid of P. multiflorum with induction rate as high as 16.7%.


CONCLUSIONS
Rapid propagation of P. multiflorum could be achieved by tissue culture. Furthermore, colchicine was an effective inducer of polyploidy, and 25 tetraploid lines were obtained through chromosome identification. The experiment laid a foundation for the wild resource conservation, superior varieties breeding of P. multiflorum.
Anhui University of Traditional Chinese Medicine, Hefei 230031, China.
He-Ping
HP
Huang
Shan-Lin
SL
Gao
Jian
J
Wang
Lu-Qi
LQ
Huang
Peng
P
Huang
D032461:Chromosomes, Plant / Q000235:genetics; D003470:Culture Media / Q000378:metabolism; D028502:Polygonum / Q000235:genetics* / Q000254:growth & development* / Q000378:metabolism; D057891:Tetraploidy; D046509:Tissue Culture Techniques / Q000379:methods*
D003470:Culture Media