[Effect of Shenlian extracts on blood flow and vessel pathological changes in rabbits carotid atherosclerosis model induced by low shear stress].
Lipid accumulation in the vessel wall and tunica intima vasorum pathological changes are important factors in the development of atherosclerosis, which are closely related with hemodynamics. In this paper, we established a model of local low shear stress in rabbits using carotid artery cannula and a high cholesterol diet for 2 weeks, 4 weeks and 8 weeks. The effects of Shenlian extract on blood flow, vascular pathology formation and lipid metabolism were assessed by electromagnetic blood flow meter and hematoxylin-eosin staining of the proximal end in carotid artery at different times. The results demonstrate that the relationship between blood flow and shear stress for control, atorvastatin, Shenlian extract high-dose, Shenlian extract middle-dose, and Shenlian extract low-dose were linearly related. The blood flow and the shear stress of proximal end in carotid artery of Shenlian extract (1.12, 2.24, 4.48 g x kg(-1)), and atorvastatin (4.7 x 10(-4) g x kg(-1)) were significantly (P < 0.05)increased compared with the control. Plasma total cholesterol (TC), low density lipoprotein cholesterol (LDL-C) ,and high density lipoprotein cholesterol (HDL-C) were markedly decreased with the increasing of dose and time. This study is the first to prove that the inhibition of Shenlian extract on low shear stress (LSS) induces rabbits carotid atherosclerosis with increasing blood flow and decreasing lipids and vessel pathological changes.
Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China. zyx-syww@sina.com
Shu-Yuan
SY
Zhou
Ying-Han
YH
Wang
Yu-Jie
YJ
Li
Qing
Q
Yang
Zi-Peng
ZP
Gong
Cong-Xiao
CX
Ruan
Xiao-Xi
XX
Kan
Rui-Jie
RJ
Zhang
Xiao-Xin
XX
Zhu
D000818:Animals; D001696:Biomechanical Phenomena; D001783:Blood Flow Velocity / Q000187:drug effects; D002339:Carotid Arteries / Q000737:chemistry / Q000187:drug effects / Q000473:pathology / Q000503:physiopathology; D002340:Carotid Artery Diseases / Q000188:drug therapy* / Q000473:pathology / Q000503:physiopathology; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage*; D006801:Humans; D008297:Male; D011817:Rabbits; D013314:Stress, Mechanical
D004365:Drugs, Chinese Herbal