Galangin induces human colon cancer cell death via the mitochondrial dysfunction and caspase-dependent pathway.
Galangin is a member of flavonols and found in Alpinia officinarum, galangal root, and propolis. Previous studies have demonstrated that galangin has anti-cancer effects on several cancers, including melanoma, hepatoma, and leukaemia cells. However, anti-cancer activity of galangin on human colon cancer has not been established yet. In this study, we investigated the anti-cancer effects of galangin on two types of human colon cancer cells (HCT-15 and HT-29). We found that galangin induced apoptosis and DNA condensation of human colon cancer cells in a dose-dependent manner. We also determined that galangin increased the activation of caspase-3 and -9, and release of apoptosis inducing factor from the mitochondria into the cytoplasm by Western blot analysis. In addition, galangin induced human colon cancer cell death through the alteration of mitochondria membrane potential and dysfunction. These results suggest that galangin induces apoptosis of HCT-15 and HT-29 human colon cancer cells and may prove useful in the development of therapeutic agents for human colon cancer.
Department of Surgery, Inje University College of Medicine, Busan Paik Hospital, Busan 633-165, South Korea.
Tae Kwun
TK
Ha
Mi Eun
ME
Kim
Ju Hwa
JH
Yoon
Sung Jin
SJ
Bae
Jihye
J
Yeom
Jun Sik
JS
Lee
D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3 / Q000378:metabolism; D053453:Caspase 9 / Q000378:metabolism; D045744:Cell Line, Tumor; D003110:Colonic Neoplasms / Q000473:pathology*; D003593:Cytoplasm / Q000378:metabolism; D005260:Female; D005419:Flavonoids / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D008297:Male; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D008928:Mitochondria / Q000187:drug effects*; D015398:Signal Transduction
D000972:Antineoplastic Agents, Phytogenic; D005419:Flavonoids; C037032:galangin; D053148:Caspase 3; D053453:Caspase 9
Human colon cancer; apoptosis; caspase activation; chromatin condensation; galangin