Selective fluorescent detection of aspartic acid and glutamic acid employing dansyl hydrazine dextran conjugate.
Highly water soluble polymer (DD) was prepared and evaluated for its fluorescence response towards various amino acids. The polymer consists of dansyl hydrazine unit conjugated into dextran template. The conjugation enhances higher water solubility of dansyl hydrazine moiety. Of screened amino acids, DD exhibited selective fluorescence quenching in the presence of aspartic acid (Asp) and glutamic acid (Glu). A plot of fluorescence intensity change of DD against the concentration of corresponding amino acids gave a good linear relationship in the range of 1 × 10(-4) M to 25 × 10(-3) M. This establishes DD as a potential polymeric sensor for selective sensing of Asp and Glu.
Department of Chemistry, Faculty of Science, Mahidol University, Rama 6 Road, Phyathai, Bangkok, 10400, Thailand.
Weerachai
W
Nasomphan
Pramuan
P
Tangboriboonrat
Sutipa
S
Tanapongpipat
Srung
S
Smanmoo
D001224:Aspartic Acid / Q000032:analysis*; D003619:Dansyl Compounds / Q000737:chemistry*; D003911:Dextrans / Q000737:chemistry*; D005453:Fluorescence; D018698:Glutamic Acid / Q000032:analysis*; D006834:Hydrazines / Q000737:chemistry*; D015394:Molecular Structure; D013050:Spectrometry, Fluorescence
D003619:Dansyl Compounds; D003911:Dextrans; D006834:Hydrazines; D001224:Aspartic Acid; C027913:dansyl hydrazine; D018698:Glutamic Acid