TaCIPK29, a CBL-interacting protein kinase gene from wheat, confers salt stress tolerance in transgenic tobacco.
Calcineurin B-like protein-interacting protein kinases (CIPKs) have been found to be responsive to abiotic stress. However, their precise functions and the related molecular mechanisms in abiotic stress tolerance are not completely understood, especially in wheat. In the present study, TaCIPK29 was identified as a new member of CIPK gene family in wheat. TaCIPK29 transcript increased after NaCl, cold, methyl viologen (MV), abscisic acid (ABA) and ethylene treatments. Over-expression of TaCIPK29 in tobacco resulted in increased salt tolerance, which was demonstrated by higher germination rates, longer root lengths and better growth status of transgenic tobacco plants compared to controls when both were treated with salt stress. Physiological measurements indicated that transgenic tobacco seedlings retained high K(+)/Na(+) ratios and Ca(2+) content by up-regulating some transporter genes expression and also possessed lower H2O2 levels and reduced membrane injury by increasing the expression and activities of catalase (CAT) and peroxidase (POD) under salt stress. Moreover, transgenic lines conferred tolerance to oxidative stress by increasing the activity and expression of CAT. Finally, TaCIPK29 was located throughout cells and it preferentially interacted with TaCBL2, TaCBL3, NtCBL2, NtCBL3 and NtCAT1. Taken together, our results showed that TaCIPK29 functions as a positive factor under salt stress and is involved in regulating cations and reactive oxygen species (ROS) homeostasis.
The Genetic Engineering International Cooperation Base of Chinese Ministry of Science and Technology, Chinese National Center of Plant Gene Research (Wuhan) HUST Part, Key Laboratory of Molecular Biophysics MoE, College of Life Science and Technology, Huazhong University of Science & Technology (HUST), Wuhan, China.
Xiaomin
X
Deng
Wei
W
Hu
Shuya
S
Wei
Shiyi
S
Zhou
Fan
F
Zhang
Jiapeng
J
Han
Lihong
L
Chen
Yin
Y
Li
Jialu
J
Feng
Bin
B
Fang
Qingchen
Q
Luo
Shasha
S
Li
Yunyi
Y
Liu
Guangxiao
G
Yang
Guangyuan
G
He
D000040:Abscisic Acid / Q000494:pharmacology; D002374:Catalase / Q000378:metabolism; D005030:Ethylenes / Q000494:pharmacology; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D006861:Hydrogen Peroxide / Q000378:metabolism; D010269:Paraquat / Q000494:pharmacology; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D012965:Sodium Chloride / Q000494:pharmacology*; D014026:Nicotiana / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism*; D014908:Triticum / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism*
D005030:Ethylenes; D010940:Plant Proteins; D017382:Reactive Oxygen Species; D012965:Sodium Chloride; D000040:Abscisic Acid; C036216:ethylene; D006861:Hydrogen Peroxide; D002374:Catalase; D010269:Paraquat