Transcriptome analysis of neuropeptides and G-protein coupled receptors (GPCRs) for neuropeptides in the brown planthopper Nilaparvata lugens.
The genes encoding neuropeptides, neurohormones and their putative G-protein coupled receptors were identified in the brown planthopper (BPH), Nilaparvata lugens (Stål) by transcriptome analysis (RNA-seq). Forty-eight candidate genes were found to encode neuropeptides or peptide hormones. These include all known insect neuropeptides and neurohormones, with the exception of neuropeptide-like precursor 2 (NPLP2) and trissin. The gene coding for prothoracicotropic hormone (PTTH) was first identified from hemimetabolous insect. A total of 57 putative neuropeptide GPCR genes were identified and phylogenetic analysis showed most of them to be closely related to insect GPCRs. A notable finding was the occurrence of vertebrate hormone receptors, thyrotropin-releasing hormone receptor (TRHR)-like GPCR and parathyroid hormone receptor (PTHR)-like GPCRs. These results suggest that N. lugens possesses the most comprehensive neuropeptide system yet found in insects. Moreover, our findings demonstrate the power of RNA-seq as a tool for analyzing the neuropeptide-related genes in the absence of whole genome sequence information.
Insect Growth Regulation Research Unit, Division of Insect Sciences, National Institute of Agribiological Sciences (NIAS), Tsukuba 3058634, Japan. Electronic address: yoshiaki@affrc.go.jp.
Yoshiaki
Y
Tanaka
Insect Genome Research Unit, Agrogenomics Research Center, NIAS, Tsukuba 3058634, Japan.
Yoshitaka
Y
Suetsugu
Insect Genome Research Unit, Agrogenomics Research Center, NIAS, Tsukuba 3058634, Japan.
Kimiko
K
Yamamoto
Division of Insect Sciences, NIAS, Tsukuba 3058634, Japan.
Hiroaki
H
Noda
Insect Growth Regulation Research Unit, Division of Insect Sciences, National Institute of Agribiological Sciences (NIAS), Tsukuba 3058634, Japan.
Tetsuro
T
Shinoda
D000818:Animals; D006430:Hemiptera / Q000235:genetics*; D007301:Insect Hormones / Q000235:genetics; D019476:Insect Proteins / Q000235:genetics*; D009479:Neuropeptides / Q000145:classification / Q000235:genetics*; D010802:Phylogeny; D043562:Receptors, G-Protein-Coupled / Q000145:classification / Q000235:genetics*
D007301:Insect Hormones; D019476:Insect Proteins; D009479:Neuropeptides; D043562:Receptors, G-Protein-Coupled; C009089:prothoracicotropic hormone
G-protein coupled receptor (GPCR); Neuropeptide; Nilaparvata lugens; Parathyroid hormone receptor (PTHR); Prothoracicotropic hormone (PTTH); Thyrotropin-releasing hormone receptor (TRHR)