Transgenes and protein localization: myths and legends.
Fluorescent protein (FP) fusions are frequently used to localize and follow the movement of proteins in living cells. However, a consensus is missing about the experimental design and controls that guarantee the reliability of the results. Here, we discuss possible artifacts and try to navigate through the many methods, preferences, and assumptions that surround protein localization in plants that make it difficult to design a universal approach to achieve reliable results.
Department of Molecular Cell Biology, Graduate School of Experimental Plant Sciences, VU-University, Amsterdam, The Netherlands. f.m.quattrocchio@vu.nl
Francesca M
FM
Quattrocchio
Cornelis
C
Spelt
Ronald
R
Koes
D015870:Gene Expression; D005819:Genetic Markers; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins; D059828:Plant Cells / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D010944:Plants / Q000235:genetics / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D021381:Protein Transport; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism*; D015203:Reproducibility of Results; D012107:Research Design; D019076:Transgenes
D005819:Genetic Markers; D010940:Plant Proteins; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins
CaMV35S promoter; GFP fusions; protein localization; transgenes