Effect of CLC-2 on the cytoskeleton in human trabecular meshwork cells.
The chloride channel protein 2 (CLC‑2) is important in maintaining the volume of trabecular meshwork cells by adjusting the outflow of aqueous solutions and maintaining the fluid balance. However, little is known concerning the functions of CLC‑2 in the cytoskeleton, specifically in human trabecular meshwork (HTM) cells. In the present study, two CLC-2 specific siRNAs (siRNA1 and siRNA2) that target CLC-2 mRNA were designed. The siRNAs were transfected into the HTM cells and the results showed that siRNA1 in particular decreased the expression of CLC‑2 by ~45%. Furthermore, an siRNA1‑mediated CLC-2 knockdown significantly reconstructed the actin cytoskeleton and formed cross‑linked actin networks. In addition, the downregulation of the expression of CLC‑2 was associated with increased TGF‑β and Smad2 activities in the HTM cells following 24 h of transfection. In conclusion, these results suggest that CLC‑2 knockdown promotes trabecular meshwork cytoskeletal disorders and may activate the TGF‑β/Smad signaling pathway. Thus, CLC‑2 may be a promising and potential novel therapeutic strategy for combating primary open‑angle glaucoma.
Department of Ophthalmology, The Second Hospital of Jilin University, Jilin University, Changchun, Jilin 130041, P.R. China.
Hong Wei
HW
Wang
Ya Juan
YJ
Zheng
D000199:Actins / Q000378:metabolism; D000090007:CLC-2 Chloride Channels; D002478:Cells, Cultured; D018118:Chloride Channels / Q000235:genetics / Q000378:metabolism*; D003599:Cytoskeleton / Q000378:metabolism; D015870:Gene Expression; D055785:Gene Knockdown Techniques; D005901:Glaucoma / Q000473:pathology; D006801:Humans; D034741:RNA, Small Interfering / Q000235:genetics; D015398:Signal Transduction; D051785:Smad Proteins / Q000378:metabolism; D022502:Stress Fibers / Q000378:metabolism*; D014129:Trabecular Meshwork / Q000473:pathology*; D016212:Transforming Growth Factor beta / Q000378:metabolism; D016596:Vinculin / Q000378:metabolism; D051176:beta Catenin / Q000378:metabolism
D000199:Actins; D000090007:CLC-2 Chloride Channels; C495270:CTNNB1 protein, human; D018118:Chloride Channels; D034741:RNA, Small Interfering; D051785:Smad Proteins; D016212:Transforming Growth Factor beta; C488955:VCL protein, human; D051176:beta Catenin; D016596:Vinculin