Labeling of human hepatocellular carcinoma cells by hexamethylene diamine modified fluorescent carbon dots.
Fluorescent carbon dots (CDs) were synthesized by a solvothermal method with glucose as carbon source and surface-modified with 1,6-hexamethylene diamine. In this hybrid CDs, the modification played important role for improving the fluorescent performance by introducing nitrogenous compound to passivate CD's surface, making the CDs emit strong fluorescence. The as-prepared CDs were linked with mouse anti-human Alpha fetoprotein (AFP) antibody and goat anti-mouse immunoglobulin (IgG) to directly and indirectly label fixed human hepatocellular carcinoma cells, respectively. The cytotoxicity of these CDs were also tested using the human hepatocellular carcinoma cells. No apparent cytotoxicity was observed, which suggested the potential application of the as-prepared CDs in bioimaging.
Department of Chemistry, Shenyang Medical College, Shenyang 110034, China. Electronic address: dongwei5873@126.com.
Wei
W
Dong
Yan
Y
Dong
Ying
Y
Wang
Shiqi
S
Zhou
Xin
X
Ge
Lili
L
Sui
Jingwen
J
Wang
D000818:Animals; D000906:Antibodies / Q000737:chemistry / Q000276:immunology; D006528:Carcinoma, Hepatocellular / Q000175:diagnosis*; D045744:Cell Line, Tumor; D003959:Diamines / Q000737:chemistry / Q000633:toxicity; D005456:Fluorescent Dyes / Q000737:chemistry / Q000633:toxicity; D006801:Humans; D008113:Liver Neoplasms / Q000175:diagnosis*; D051379:Mice; D045663:Quantum Dots / Q000737:chemistry / Q000633:toxicity; D000509:alpha-Fetoproteins / Q000276:immunology
D000906:Antibodies; D003959:Diamines; D005456:Fluorescent Dyes; D000509:alpha-Fetoproteins
Cytotoxicity; Fluorescence spectra; Fluorescent carbon dots; Human hepatocellular carcinoma cells