[Effects of endothelial progenitor cell-conditioned medium on the proliferation, adhesion and migration of vascular smooth muscle cells].
OBJECTIVE
To investigate the effects of endothelia progenitor cells conditioned medium (EPC-CM) on the migration, adhesion and proliferation of vascular smooth muscle cells (VSMCs).


METHODS
Mononuclear cells were isolated from rat bone marrow by density gradient centrifugation,plated on dishes precoated with 5% fibronectin, and then cultured with complete M199 medium (including 15% fetal calf serum, 10 microg/L VEGF and 5 microg/L bFGF). EPC-CM was collected and used to incubate VSMCs isolated from rat arteriae aorta. After 24 h, VSMCs proliferation, adhesion and migration were assayed with CCK-8, adhesion test and modified Boyden chamber assay, respectively.


RESULTS
The proliferation, adhesion and migration of VSMCs were obviously decreased when the cells were cultured with EPC-CM.


CONCLUSIONS
EPC-CM could inhibit VSMC functions, which would be one of the mechanisms against atherosclerosis by EPCs.
Department of Anesthesiology, Weifang Medical University, Weifang 261053, China.
Hai-Yan
HY
Wu
Hai-Rong
HR
Chu
Hong
H
Li
Ke-Xin
KX
Tang
Xu
X
Jing
Jin-Long
JL
Sun
Qing-Ling
QL
Yin
Min
M
Cheng
D000818:Animals; D002448:Cell Adhesion; D002465:Cell Movement; D049109:Cell Proliferation; D002478:Cells, Cultured; D017077:Culture Media, Conditioned / Q000737:chemistry*; D042783:Endothelial Cells / Q000166:cytology*; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000166:cytology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013234:Stem Cells / Q000166:cytology*
D017077:Culture Media, Conditioned