[Study on protective effect of cerebrospinal fluid containing Qingxin Kaiqiao Fang on sodium dithionite-induced PC12 cell injury].
OBJECTIVE
To study the protective effect of cerebrospinal fluid containing Qingxin Kaiqiao Fang on sodium dithionite (Na2S2O4)-induced PC12 cell injury, in order to provide basis for clinical application of the prescription.


METHODS
SD rats were orally administered with water decoction of Qingxin Kaiqiao Fang (7. 9 g . kg-1) once every 12 h, for a total of 7 times, in order to prepare cerebrospinal fluid containing Qingxin Kaiqiao Fang. The neurocyte injury model was established by adding Na2S2O4 with the final concentration of 8 m mol . L-1 into PC12 cells. With nimodipine (1 x 10(7)mol . L-1 ) as the positive control group, MTT method test was adopted to detect the impact of cerebrospinal fluid containing Qingxin Kaiqiao Fang on the activity of PC12 cells. The expression of Bax, Bel-2 and Caspase-3 mRNA was detected by RT-PCR.


RESULTS
The cerebrospinal fluid containing Qingxin Kaiqiao Fang groups showed a significantly higher activity in PC12 cells than the model group, with decrease in expressions of Bax mRNA and Caspase-3 mRNA and increase in expression of Bel-2 mRNA. There were significant differences compared with the model group (P< 0. 05,P <0. 01).


CONCLUSIONS
Qingxin Kaiqiao Fang shows a notable protective effect on Na2S2 04-induced neurocyte injury.
The Second Clinical Medical College of Wenzhou Medical College, Wenzhou 325003, China. wyzhongyixi@163.com
Hai-Yan
HY
Hu
Zhi-Hui
ZH
Cui
Xiang
X
Chen
Wen-Hua
WH
Wang
Xiao-Yan
XY
Zhang
D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002555:Cerebrospinal Fluid / Q000737:chemistry*; D004227:Dithionite / Q000633:toxicity*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D016716:PC12 Cells; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley
D004365:Drugs, Chinese Herbal; D019253:Proto-Oncogene Proteins c-bcl-2; D004227:Dithionite