Portable, miniaturized, fibre delivered, multimodal CARS exoscope.
We demonstrate for the first time, a portable multimodal coherent anti-Stokes Raman scattering microscope (exoscope) for minimally invasive in-vivo imaging of tissues. This device is based around a micro-electromechanical system scanning mirror and miniaturized optics with light delivery accomplished by a photonic crystal fibre. A single Ti:sapphire femtosecond pulsed laser is used as the light source to produce CARS, two photon excitation fluorescence and second harmonic generation images. The high resolution and distortion-free images obtained from various resolution and bio-samples, particularly in backward direction (epi) successfully demonstrate proof of concept, and pave the path towards future non or minimally-invasive in vivo imaging.
School of Electrical Engineering and Computer Science, University of Ottawa, 800 King Edward, PO Box450 Stn A, Ottawa, Ontario K1N 6N5, Canada. bsmit021@uottawa.ca
Brett
B
Smith
Majid
M
Naji
Sangeeta
S
Murugkar
Emilio
E
Alarcon
Craig
C
Brideau
Peter
P
Stys
Hanan
H
Anis
D004867:Equipment Design; D019544:Equipment Failure Analysis; D005336:Fiber Optic Technology / Q000295:instrumentation*; D007834:Lasers; D055617:Micro-Electrical-Mechanical Systems / Q000295:instrumentation*; D036641:Microscopy, Fluorescence, Multiphoton / Q000379:methods*; D008904:Miniaturization; D064847:Multimodal Imaging / Q000295:instrumentation*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013059:Spectrum Analysis, Raman / Q000295:instrumentation*