Increase in stearidonic acid by increasing the supply of histidine to oleaginous Saccharomyces cerevisiae.
Increasing concentration of histidine significantly increased stearidonic acid production and cell growth in oleaginous Saccharomyces cerevisiae that has been genetically modified by Δsnf2 disruption, DGA1 and Δ6 desaturase gene overexpression, and LEU2 expression. High concentration of histidine in wild-type transformant and HIS3 expression in Δsnf2 transformant also increased stearidonic acid.
Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Higashi 1-1-1, Tsukuba, Ibaraki 305-8566, Japan. Electronic address: kykimura@ni.aist.go.jp.
Kazuyoshi
K
Kimura
Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Higashi 1-1-1, Tsukuba, Ibaraki 305-8566, Japan.
Yasushi
Y
Kamisaka
Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Higashi 1-1-1, Tsukuba, Ibaraki 305-8566, Japan.
Hiroshi
H
Uemura
Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Higashi 1-1-1, Tsukuba, Ibaraki 305-8566, Japan.
Masakazu
M
Yamaoka
D050539:3-Isopropylmalate Dehydrogenase / Q000235:genetics / Q000378:metabolism; D000251:Adenosine Triphosphatases / Q000235:genetics / Q000378:metabolism; D051048:Diacylglycerol O-Acyltransferase / Q000235:genetics / Q000378:metabolism; D015525:Fatty Acids, Omega-3 / Q000378:metabolism*; D006639:Histidine / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
D015525:Fatty Acids, Omega-3; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D006639:Histidine; D050539:3-Isopropylmalate Dehydrogenase; C524993:LEU2 protein, S cerevisiae; C494880:DGA1 protein, S cerevisiae; D051048:Diacylglycerol O-Acyltransferase; D000251:Adenosine Triphosphatases; C499333:SNF2 protein, S cerevisiae; C062895:stearidonic acid
DGA1; Histidine; Polyunsaturated fatty acid; Stearidonic acid; Δ6 Desaturase; Δsnf2; α-Linolenic acid