Molecular cloning and functional characterization of a short peptidoglycan recognition protein (HcPGRPS1) from the freshwater mussel, Hyriopsis cumingi.
Peptidoglycan recognition proteins (PGRPs), which are evolutionarily conserved from invertebrates to vertebrates, function as pattern-recognition and effector molecules in innate immunity. In the present study, a short-form PGRP, designated as HcPGRPS1 was identified from freshwater mussel Hyriopsis cumingi. The deduced amino acid sequence of HcPGRPS1 is composed of 235 residues which contains a conserved PGRP domain at the C-terminus. Sequence analysis showed that HcPGRPS1 shared high identities with other known PGRPs. The mRNA of HcPGRPS1 is constitutively expressed in a wide range of all tested tissues, with highest expression level in hepatopancreas, and its expression in tissues (gonad, nephridium, gill and foot) was up-regulated significantly after LPS or PGN stimulation (P<0.05). The recombinant protein of HcPGRPS1 exhibited binding activity and peptidoglycan-lytic amidase activity toward Lys-PGN from Staphylococcus aureus and DAP-PGN from Bacillus subtilis. Furthermore, recombinant HcPGRPS1 displayed strong antibacterial activity to both Gram-negative bacteria Escherichia coli, Aeromonas hydrophila, Aeromonas sobria and Gram-positive bacteria S. aureus in the presence of Zn(2+). These results suggested that HcPGRPS1 plays a multifunctional role in the defense and protection mechanisms of mussel innate immunity against infections.
School of Environmental Science and Engineering, Huazhong University of Science and Technology, Wuhan, Hubei Province 430074, China; School of Environment and Municipal Engineering, North China University of Water Resources and Electric Power, Zhengzhou, Henan Province 450011, China.
Ziyan
Z
Yang
Junhua
J
Li
Ying
Y
Li
Hongjuan
H
Wu
Xiaoyan
X
Wang
D000581:Amidohydrolases / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D049872:Bivalvia / Q000235:genetics* / Q000378:metabolism; D015153:Blotting, Western; D002352:Carrier Proteins / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D005618:Fresh Water; D015870:Gene Expression / Q000187:drug effects; D020869:Gene Expression Profiling; D006090:Gram-Negative Bacteria / Q000187:drug effects; D006094:Gram-Positive Bacteria / Q000187:drug effects; D043143:Hepatopancreas / Q000378:metabolism*; D007155:Immunologic Factors / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology; D008969:Molecular Sequence Data; D010457:Peptidoglycan / Q000378:metabolism / Q000494:pharmacology; D010802:Phylogeny; D011485:Protein Binding; D011506:Proteins / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D011817:Rabbits; D011994:Recombinant Proteins / Q000276:immunology / Q000494:pharmacology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
D002352:Carrier Proteins; D018076:DNA, Complementary; D007155:Immunologic Factors; D008070:Lipopolysaccharides; D010457:Peptidoglycan; D011506:Proteins; D011994:Recombinant Proteins; C118897:peptidoglycan recognition protein; D000581:Amidohydrolases; C019665:amidase
Amidase activity; Antibacterial activity; Hyriopsis cumingi; Innate immunity; Peptidoglycan recognition protein; Peptidoglycan-binding activity