Molecular diagnostics for the detection of Bokeloh bat lyssavirus in a bat from Bavaria, Germany.
A brain sample of a Natterer's bat tested positive for rabies with classical virological techniques. Molecular techniques confirmed the presence of Bokeloh bat lyssavirus (BBLV) in Germany for the second time. Sequence analysis revealed a close genetic relationship to the initial German BBLV case. Using a TaqMan RT-PCR specific for BBLV viral RNA was detected in various other organs albeit with differences in the relative viral load.
Friedrich-Loeffler-Institut (FLI), Federal Research Institute for Animal Health, Institute of Molecular Biology, WHO Collaborating Centre for Rabies Surveillance and Research, D-17493 Greifswald, Insel Riems, Germany.
Conrad M
CM
Freuling
Björn
B
Abendroth
Martin
M
Beer
Melina
M
Fischer
Dennis
D
Hanke
Bernd
B
Hoffmann
Dirk
D
Höper
Frank
F
Just
Thomas C
TC
Mettenleiter
Juliane
J
Schatz
Thomas
T
Müller
D000818:Animals; D001483:Base Sequence; D002685:Chiroptera / Q000821:virology*; D005858:Germany; D018114:Lyssavirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D010802:Phylogeny; D018353:Rhabdoviridae Infections / Q000175:diagnosis / Q000662:veterinary* / Q000821:virology
Bat rabies; Bokeloh bat lyssavirus; Lyssaviruses; Microarray; Myotis nattereri; Realtime PCR