New opportunities for immobilization of enzymes.
In this chapter, as a general introduction, we summarize our personal point of view on immobilization technique in order to prepare optimal and cost-effective biocatalysts. Special attention is paid to the improvement of enzyme properties via immobilization techniques. From the stabilization by multipoint covalent attachment to the generation of hydrophilic environments via post-immobilization techniques are here discussed. Immobilization techniques, a necessary tool to reuse enzyme, have to be simple and, if possible, may become a very powerful tool to greatly improve properties of every kind of enzymes: monomeric, multimeric, stable, labile, poorly selective, etc.
Instituto de Catalisis y Petroleoquimica, CSIC, Madrid, Spain.
Jose M
JM
Guisan
D055162:Biocatalysis; D019149:Bioreactors; D003432:Cross-Linking Reagents / Q000737:chemistry; D003460:Crystallization; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry*; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D046912:Multiprotein Complexes / Q000737:chemistry; D011485:Protein Binding
D003432:Cross-Linking Reagents; D004800:Enzymes, Immobilized; D046912:Multiprotein Complexes