Generating hypoimmunogenic human embryonic stem cells by the disruption of beta 2-microglobulin.
Immune rejection hinders the application of human embryonic stem cells (hESCs) in transplantation therapy. Human leukocyte antigens (HLAs) on the cell surface are the major cause of graft rejection. In this study, we generated HLA class I-deficient hESCs via disruption of beta 2-microglobulin (β2m), the light chain of HLA Class I. We found that HLA class I proteins were not present on the cell surface of β2m-null hESCs. These cells showed the same pluripotency as wildtype hESCs and demonstrated hypoimmunogenicity. Thus, HLA class I-deficient hESCs might serve as an unlimited cell source for the generation of universally compatible "off-the-shelf" cell grafts, tissues or organs in the future.
Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, 200031, People's Republic of China.
Pengfei
P
Lu
Jijun
J
Chen
Lixiazi
L
He
Jiangtao
J
Ren
Haide
H
Chen
Lingjun
L
Rao
Qinggang
Q
Zhuang
Hui
H
Li
Lei
L
Li
Lei
L
Bao
Ji
J
He
Wei
W
Zhang
Faming
F
Zhu
Chun
C
Cui
Lei
L
Xiao
D001483:Base Sequence; D053595:Embryonic Stem Cells / Q000166:cytology / Q000276:immunology*; D015395:Histocompatibility Antigens Class I / Q000378:metabolism; D006801:Humans; D008969:Molecular Sequence Data; D039904:Pluripotent Stem Cells / Q000166:cytology / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism; D001613:beta 2-Microglobulin / Q000172:deficiency / Q000378:metabolism*
D015395:Histocompatibility Antigens Class I; D014157:Transcription Factors; D001613:beta 2-Microglobulin