Myroides guanonis sp. nov., isolated from prehistoric paintings.
A novel psychrotolerant, strictly aerobic, non-motile, rod-shaped bacterial strain, designated IM13(T), was isolated from a sample taken from prehistoric guano paintings in Magura Cave, northwest Bulgaria and subjected to a polyphasic taxonomic study. Strain IM13(T) formed yellow colonies on LB agar plates and was Gram-staining-negative, heterotrophic and alkalitolerant. It grew optimally at pH 7.5 and 30 °C in the absence of NaCl. Phylogenetic analysis of the whole 16S rRNA gene revealed that strain IM13(T) branched with representatives of the genus Myroides with sequence similarity of 93-94 % with other species of the genus. The novel isolate contained iso-C15 : 0 (49.1 %), iso-C17 : 1ω9c (18.2 %) and iso-C17 : 0 3-OH (14.0 %) as dominant fatty acids. The DNA G+C content of strain IM13(T) was 33.5 mol%. Based on phylogenetic inference and phenotypic characteristics, it was concluded that strain IM13(T) represents a novel species of the genus Myroides, for which the name Myroides guanonis sp. nov. is proposed. The type strain is IM13(T) ( = DSM 26542(T) = NBIMCC 8736(T)).
Institute of Microbiology, Bulgarian Academy of Sciences, 'Acad. G. Bonchev' Str. 26, 1113 Sofia, Bulgaria.
Anna
A
Tomova
Institute of Microbiology, Bulgarian Academy of Sciences, 'Acad. G. Bonchev' Str. 26, 1113 Sofia, Bulgaria.
Iva
I
Tomova
Institute of Microbiology, Bulgarian Academy of Sciences, 'Acad. G. Bonchev' Str. 26, 1113 Sofia, Bulgaria.
Evgenia
E
Vasileva-Tonkova
Institute of Microbiology, Bulgarian Academy of Sciences, 'Acad. G. Bonchev' Str. 26, 1113 Sofia, Bulgaria.
Irina
I
Lazarkevich
Institute of Microbiology, Bulgarian Academy of Sciences, 'Acad. G. Bonchev' Str. 26, 1113 Sofia, Bulgaria.
Margarita
M
Stoilova-Disheva
Institute of Microbiology, Bulgarian Academy of Sciences, 'Acad. G. Bonchev' Str. 26, 1113 Sofia, Bulgaria.
Dimitrinka
D
Lyutskanova
Institute of Microbiology, Bulgarian Academy of Sciences, 'Acad. G. Bonchev' Str. 26, 1113 Sofia, Bulgaria.
Margarita
M
Kambourova
D015373:Bacterial Typing Techniques; D001482:Base Composition; D002031:Bulgaria; D059552:Caves / Q000382:microbiology*; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D041965:Flavobacteriaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D008969:Molecular Sequence Data; D010151:Paintings; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S