Selective fluorescent-free detection of biomolecules on nanobiochips by wavelength dependent-enhanced dark field illumination.
Individual silver nanoparticle-conjugated target protein (cTnI) molecules on gold-nanopatterned chip were selectively detected by wavelength dependent-enhanced dark field illumination. Using specific nanoparticles with unique sizes and materials, the immunotargeted nanoparticle on the chips was detected at the single-molecule level by monitoring changes in the plasmonic resonance based on wavelength dependence.
Department of Applied Chemistry, Kyung Hee University, Yongin-si, Gyeonggi-do 446-701, Republic of Korea. shkang@khu.ac.kr.
Seungah
S
Lee
Hyunung
H
Yu
Seong Ho
SH
Kang
D055910:Antibodies, Immobilized / Q000737:chemistry; D004867:Equipment Design; D006046:Gold / Q000737:chemistry*; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation; D008027:Light; D053758:Nanoparticles / Q000737:chemistry*; D012834:Silver / Q000737:chemistry*; D020349:Surface Plasmon Resonance / Q000295:instrumentation*; D019210:Troponin I / Q000032:analysis*
D055910:Antibodies, Immobilized; D019210:Troponin I; D012834:Silver; D006046:Gold