Beyond identification: emerging and future uses for MALDI-TOF mass spectrometry in the clinical microbiology laboratory.
The routine use of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has revolutionized microorganism identification in the clinical microbiology laboratory. Building from these now common microorganism identification strategies, this review explores future clinical applications of MALDI-TOF MS. This includes practical approaches for laboratorians interested in implementing direct identification processing methods for MALDI-TOF detection of microbes in bloodstream infection (BSI) and urinary tract infection (UTI), as well as, post-analytical approaches for classifying MALDI-TOF spectral data to detect characteristics other and species-level identification (e.g. strain-level classification, typing, and resistance mechanisms).
Department of Pathology and Laboratory Medicine, University of British Columbia, St. Paul's Hospital, Vancouver, BC, V6Z 1Y6, Canada.
Mari L
ML
DeMarco
Bradley A
BA
Ford
D016470:Bacteremia / Q000175:diagnosis; D001424:Bacterial Infections / Q000175:diagnosis; D015373:Bacterial Typing Techniques / Q000639:trends; D006801:Humans; D007753:Laboratories; D040901:Proteomics / Q000295:instrumentation / Q000379:methods / Q000639:trends; D013048:Specimen Handling; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000295:instrumentation / Q000379:methods / Q000639:trends*; D014552:Urinary Tract Infections / Q000175:diagnosis / Q000382:microbiology
Antimicrobial susceptibility testing; Bacteremia; Bloodstream infection; Direct identification; MALDI-TOF; Machine learning; Mass spectrometry; Urinary tract infection