Development of gateway binary vectors R4L1pGWB possessing the bialaphos resistance gene (bar) and the tunicamycin resistance gene as markers for promoter analysis in plants.
We have developed a new series of R4L1 Gateway binary vectors (R4L1pGWB), which carry the bialaphos resistance gene (bar) or the UDP-N-acetylglucosamine:dolichol phosphate N-acetylglucosamine-1-P transferase (GPT) gene as selection markers that confer BASTA® and tunicamycin resistance on plants respectively. R4L1pGWBs have an attR4-attL1-reporter and can accept an attL4-promoter-attR1 entry clone for easy construction of an attB4-promoter-attB1-reporter clone. The new R4L1pGWBs facilitate promoter:reporter analysis in pre-existing transgenic plants that are resistant to kanamycin or hygromycin.
Department of Molecular and Functional Genomics, Center for Integrated Research in Science, Shimane University.
Yuji
Y
Tanaka
Kenta
K
Shibahara
Tsuyoshi
T
Nakagawa
D015415:Biomarkers; D002934:Cinnamates / Q000494:pharmacology; D004352:Drug Resistance, Microbial / Q000235:genetics*; D005822:Genetic Vectors; D006921:Hygromycin B / Q000031:analogs & derivatives / Q000494:pharmacology; D007612:Kanamycin / Q000494:pharmacology; D009943:Organophosphorus Compounds / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000235:genetics*; D011401:Promoter Regions, Genetic; D014415:Tunicamycin / Q000235:genetics / Q000378:metabolism; D014539:Uridine Diphosphate Sugars / Q000235:genetics*
D015415:Biomarkers; D002934:Cinnamates; D009943:Organophosphorus Compounds; D014539:Uridine Diphosphate Sugars; C083732:uridine diphosphate N-acetyllactosamine; D014415:Tunicamycin; C003122:bialaphos; D006921:Hygromycin B; C026273:hygromycin A; D007612:Kanamycin