Custom-fit ruthenium(II) metallopeptides: a new twist to DNA binding with coordination compounds.
A new bipyridine building block has been used for the solid-phase synthesis of dinuclear DNA-binding ruthenium(II) metallopeptides. Detailed spectroscopic studies suggest that these compounds bind to the DNA by insertion into the DNA minor groove. Moreover, the potential of the solid-phase peptide synthesis approach is demonstrated by the straightforward synthesis of an octaarginine derivative that shows effective cellular internalization and cytotoxicity linked with strong DNA interaction, as evidenced by steady-state fluorescence spectroscopy and AFM studies.
Departamento de Química Inorgánica y Centro Singular de Investigación en Química Biolóxica e Materiais Moleculares, Universidade de Santiago de Compostela, 15782 Santiago de Compostela (Spain).
Ilaria
I
Gamba
Iria
I
Salvadó
Gustavo
G
Rama
Miriam
M
Bertazzon
Mateo I
MI
Sánchez
Víctor M
VM
Sánchez-Pedregal
José
J
Martínez-Costas
Rosa F
RF
Brissos
Patrick
P
Gamez
José L
JL
Mascareñas
Miguel
M
Vázquez López
M Eugenio
ME
Vázquez
D015082:2,2'-Dipyridyl / Q000737:chemistry*; D001665:Binding Sites; D056831:Coordination Complexes / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D008667:Metalloproteins / Q000737:chemistry*; D012428:Ruthenium / Q000737:chemistry*; D060327:Solid-Phase Synthesis Techniques; D013050:Spectrometry, Fluorescence
D056831:Coordination Complexes; D008667:Metalloproteins; D015082:2,2'-Dipyridyl; D012428:Ruthenium; D004247:DNA
DNA recognition; fluorescence; metallopeptides; molecular recognition; supramolecular chemistry