Determination of the biochemical properties of full-length human PIF1 ATPase.
The PIF1 helicase family performs many cellular functions. To better understand the functions of the human PIF1 helicase, we characterized the biochemical properties of its ATPase. PIF1 is very sensitive to temperature, whereas it is not affected by pH, and the ATPase activity of human PIF1 is dependent on the divalent cations Mg (2+) and Mn (2+) but not Ca (2+) and Zn (2+). Inhibition was observed when single-stranded DNA was coated with RPA or SSB. Moreover, the ATPase activity of PIF1 proportionally decreased with decreasing oligonucleotide length due to a decreased binding ability. A minimum of 10 oligonucleotide bases are required for PIF1 binding and the hydrolysis of ATP. The analysis of the biochemical properties of PIF1 together with numerous genetic observations should aid in the understanding of its cellular functions.
Department of Radiation Toxicology and Oncology; Beijing Institute of Radiation Medicine; Beijing, PR China; School of Medicine; Shihezi University; Shihezi, PR China.
Yongqing
Y
Gu
Jianxiao
J
Wang
Shanshan
S
Li
Kenji
K
Kamiya
Xiaohua
X
Chen
Pingkun
P
Zhou
D000255:Adenosine Triphosphate / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D002413:Cations, Divalent / Q000737:chemistry* / Q000378:metabolism; D004265:DNA Helicases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D006801:Humans; D006868:Hydrolysis; D008274:Magnesium / Q000737:chemistry* / Q000378:metabolism; D008345:Manganese / Q000737:chemistry* / Q000378:metabolism; D009841:Oligonucleotides / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D011485:Protein Binding; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
D002413:Cations, Divalent; D009841:Oligonucleotides; D011994:Recombinant Proteins; D008345:Manganese; D000255:Adenosine Triphosphate; D004265:DNA Helicases; C508765:PIF1 protein, human; D008274:Magnesium
ATPase; PIF1 helicase; single-stranded DNA binding