A novel method for rapid hybridization of DNA to a solid support.
Here we present a novel approach entitled Magnetic Forced Hybridization (MFH) that provides the means for efficient and direct hybridization of target nucleic acids to complementary probes immobilized on a glass surface in less than 15 seconds at ambient temperature. In addition, detection is carried out instantly since the beads become visible on the surface. The concept of MFH was tested for quality control of array manufacturing, and was combined with a multiplex competitive hybridization (MUCH) approach for typing of Human Papilloma Virus (HPV). Magnetic Forced Hybridization of bead-DNA constructs to a surface achieves a significant reduction in diagnostic testing time. In addition, readout of results by visual inspection of the unassisted eye eliminates the need for additional expensive instrumentation. The method uses the same set of beads throughout the whole process of manipulating and washing DNA constructs prior to detection, as in the actual detection step itself.
Division of Gene Technology, Science for Life Laboratory, Royal Institute of Technology, Stockholm, Sweden.
Erik
E
Pettersson
Afshin
A
Ahmadian
Patrik L
PL
Ståhl
D004247:DNA; D060005:Genotyping Techniques / Q000379:methods; D006801:Humans; D060526:Magnetic Fields; D009693:Nucleic Acid Hybridization / Q000379:methods*; D015345:Oligonucleotide Probes; D027383:Papillomaviridae / Q000235:genetics; D012680:Sensitivity and Specificity
D015345:Oligonucleotide Probes; D004247:DNA