Valency-dependent affinity of bioactive hydroxyapatite-binding dendrons.
Hydroxyapatite (HA)-coated surfaces are used widely as stationary phase for protein and enzyme purification, coatings for dental and orthopedic implants, and composite materials for tissue engineering substrates. More advanced applications are envisioned, but progress has been slowed by the limited ability to controllably functionalize the surface of HA with biomolecules in a translationally relevant manner. Herein we report the synthesis and characterization of a series of multivalent, HA-binding peptide bioconjugates with variable valency and tether length which afford the ability to precisely tune the desired binding behavior. The respective binding affinities of the multivalent constructs to HA surface were characterized by quartz crystal microbalance with dissipation monitoring (QCM-D) techniques, and the relationship between dendron structure and binding affinity was revealed. Tetravalent constructs of HA-binding peptides show a 100-fold enhancement in binding affinity compared to HA-binding peptide sequences reported previously. Both biotin and bone morphogenic protein-2 (BMP-2) derivative peptide were successfully linked to the focal point as initial demonstrations.
Department of Polymer Science, The University of Akron, Akron, Ohio 44325, United States.
Wen
W
Tang
Yanrui
Y
Ma
Sibai
S
Xie
Kai
K
Guo
Bryan
B
Katzenmeyer
Chrys
C
Wesdemiotis
Matthew L
ML
Becker
D000327:Adsorption; D000595:Amino Acid Sequence; D001710:Biotin / Q000737:chemistry; D055396:Bone Morphogenetic Protein 2 / Q000737:chemistry; D020099:Coated Materials, Biocompatible / Q000737:chemistry; D061565:Cycloaddition Reaction; D050091:Dendrimers / Q000737:chemistry*; D006882:Hydroxyapatites / Q000737:chemistry*; D055767:Immobilized Proteins / Q000737:chemistry; D008968:Molecular Conformation; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry; D011485:Protein Binding; D058525:Quartz Crystal Microbalance Techniques; D013499:Surface Properties; D013816:Thermodynamics; D023822:Tissue Engineering
C525718:BMP2 protein, human; D055396:Bone Morphogenetic Protein 2; D020099:Coated Materials, Biocompatible; D050091:Dendrimers; D006882:Hydroxyapatites; D055767:Immobilized Proteins; D010455:Peptides; D001710:Biotin