Isolation and culture of precursor cells from the adult human spinal cord.
Our group recently provided evidence for the presence of neural stem cells and/or progenitor cells in the adult human spinal cord. In this chapter, we review materials and methods to harvest high-quality samples of thoracolumbar, lumbar, and sacral adult human spinal cord from brain-dead patients who had agreed to donate their bodies to science for therapeutic and scientific advances. The methods to culture precursor cells from the adult human spinal cord are also described.
Département de Neurochirurgie, Centre Hospitalier Universitaire, Hôpital Gui de Chauliac, Montpellier, France.
Luc
L
Bauchet
Nicolas
N
Lonjon
Florence
F
Vachiery-Lahaye
Alain
A
Boularan
Alain
A
Privat
Jean-Philippe
JP
Hugnot
D000328:Adult; D002469:Cell Separation; D004210:Dissection; D006801:Humans; D058953:Neural Stem Cells / Q000166:cytology*; D061251:Primary Cell Culture; D018874:Spheroids, Cellular / Q000166:cytology; D013116:Spinal Cord / Q000166:cytology*; D009927:Tissue and Organ Procurement