Pulsed-focused ultrasound enhances boron drug accumulation in a human head and neck cancer xenograft-bearing mouse model.
OBJECTIVE
This study aims to demonstrate that pulsed high-intensity focused ultrasound (pulsed-HIFU) may enhance the fructose-conjugated 4-borono-L-phenylalanine (BPA-Fr) accumulation in tumor lesion using (18)F-FBPA-Fr microPET scans.


METHODS
To the mice bearing orthotopic SASC03 human tongue squamous carcinoma xenograft, a 2-min pulsed-HIFU was applied to tumor. Immediately after pulsed-HIFU treatment, (18)F-FBPA-Fr was intravenously injected, and biological characterizations including microPET imaging and biodistribution were conducted.


RESULTS
Both biodistribution studies and microPET imaging performed after intravenous injection of (18)F-FBPA-Fr revealed higher tumor uptake in HIFU-treated mice than that of the control. CD31 and Ki-67 histochemical staining of tumor sections and H&E staining of nearby normal tissues revealed no significant difference between the pulsed-HIFU-treated mice and the control.


CONCLUSIONS
This study demonstrated that pulsed-HIFU was beneficial to the accumulation of boron drug in the head and neck tumor lesion and may enhance the therapeutic efficacy of clinical BNCT.
Department of Biomedical Imaging and Radiological Sciences, National Yang-Ming University, No.155, Sec.2, Li-Nong St, 11217, Taipei, Taiwan.
Chun-Yi
CY
Wu
Pei-Chia
PC
Chan
Lin-Shan
LS
Chou
Chi-Wei
CW
Chang
Feng-Yi
FY
Yang
Ren-Shyan
RS
Liu
Shih-Hwa
SH
Chiou
Yi-Wei
YW
Chen
Sang-Hue
SH
Yen
Hsin-Ell
HE
Wang
D000818:Animals; D019540:Area Under Curve; D001896:Boron Compounds / Q000494:pharmacology; D002294:Carcinoma, Squamous Cell / Q000000981:diagnostic imaging / Q000473:pathology; D045744:Cell Line, Tumor; D006020:Glycopeptides / Q000494:pharmacology; D006258:Head and Neck Neoplasms / Q000000981:diagnostic imaging* / Q000473:pathology; D057086:High-Intensity Focused Ultrasound Ablation; D006801:Humans; D051379:Mice; D049268:Positron-Emission Tomography; D014018:Tissue Distribution / Q000187:drug effects; D014057:Tomography, X-Ray Computed; D014062:Tongue Neoplasms / Q000000981:diagnostic imaging / Q000473:pathology; D023041:Xenograft Model Antitumor Assays
C504600:4-borono-2-fluorophenylalaninefructose; D001896:Boron Compounds; D006020:Glycopeptides