Fluorescent protein applications in microscopy.
The use of fluorescent proteins (FPs) in modern cell biology and microscopy has had an extraordinary impact on our ability to investigate dynamic processes in living cells. FPs are unique in that fluorescence is encoded solely by the primary amino acid sequence of the FP and does not require enzymatic modification or cofactors. This genetically encoded fluorescence enables the expression of FPs in diverse cells and organisms and the detection of that fluorescence in living systems. This chapter focuses on microscopy-based applications of FP detection to monitor protein localization, dynamics, interaction, and the cellular environment.
Department of Biochemistry, Stanford Medical School, Stanford University, Stanford, California, USA.
Whitney L
WL
Johnson
Aaron F
AF
Straight
D019943:Amino Acid Substitution; D000818:Animals; D002478:Cells, Cultured; D003001:Cloning, Molecular; D049452:Green Fluorescent Proteins / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics; D006801:Humans; D008856:Microscopy, Fluorescence; D008958:Models, Molecular; D016297:Mutagenesis, Site-Directed; D025941:Protein Interaction Mapping; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics; D059008:Time-Lapse Imaging
D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins
Chromophore; Fluorescent proteins; GFP; Photoactivation; Protein dynamics; Protein localization; Time-lapse imaging