In vitro lymphocyte stimulation by recombinant hepatitis B surface antigen: a tool to detect the persistence of cellular immunity after vaccination.
The study of the human immune response to hepatitis B virus (HBV) has been hampered by the lack of an adequate model to evaluate the hepatitis B surface antigen (HBsAg) specific cell response. Thus, this study was conducted to perform an in vitro analysis of the antigenic properties of recombinant HBsAg and demonstrate the influence of variables such as culture time, antigen concentration and cell density on lymphocyte proliferation. Peripheral blood mononuclear cells (PBMCs) were isolated from the venous blood of vaccinated individuals, and in vitro cellular immune responses were evaluated using an HBsAg-specific proliferation assay. Lymphoproliferative responses were detected in culture systems, despite the lack of serum antibodies. Optimal results were obtained when lymphocytes were stimulated at a seeding density of 4×10(6) cells/mL, with 50 ng/mL of recombinant HBsAg protein vaccine for 3 days. Data from the present study may contribute to the development of an adequate system to evaluate the cellular immune responses to HBsAg in vaccine recipients.
Post-Graduation Program in Pharmacy, Health Sciences Center, Federal University of Santa Catarina, University Campus, Florianópolis, Santa Catarina, Brazil.
Andréa
A
do Livramento
Janaína
J
Sampaio
Júnia
J
Schultz
Keila Zaniboni Siqueira
KZ
Batista
Arício
A
Treitinger
Caio Maurício Mendes
CM
de Cordova
Celso
C
Spada
D000293:Adolescent; D000328:Adult; D000368:Aged; D049109:Cell Proliferation; D002478:Cells, Cultured; D005260:Female; D064368:Healthy Volunteers; D006509:Hepatitis B / Q000276:immunology / Q000517:prevention & control*; D006514:Hepatitis B Surface Antigens / Q000276:immunology*; D017325:Hepatitis B Vaccines / Q000008:administration & dosage / Q000276:immunology*; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D007963:Leukocytes, Mononuclear / Q000276:immunology; D008213:Lymphocyte Activation; D008297:Male; D008875:Middle Aged; D055815:Young Adult
D006514:Hepatitis B Surface Antigens; D017325:Hepatitis B Vaccines
HBV vaccine; In vitro cellular response; Recombinant HBV surface antigen