BmCREC is an endoplasmic reticulum (ER) resident protein and required for ER/Golgi morphology.
Silkworm posterior silkgland is a model for studying intracellular trafficking. Here, using this model, we identify several potential cargo proteins of BmKinesin-1 and focus on one candidate, BmCREC. BmCREC (also known as Bombyx mori DNA supercoiling factor, BmSCF) was previously proposed to supercoil DNA in the nucleus. However, we show here that BmCREC is localized in the ER lumen. Its C-terminal tetrapeptide HDEF is recognized by the KDEL receptor, and subsequently it is retrogradely transported by coat protein I (COPI) vesicles to the ER. Lacking the HDEF tetrapeptide of BmCREC or knocking down COPI subunits results in decreased ER retention and simultaneously increased secretion of BmCREC. Furthermore, we find that BmCREC knockdown markedly disrupts the morphology of the ER and Golgi apparatus and leads to a defect of posterior silkgland tube expansion. Together, our results clarify the ER retention mechanism of BmCREC and reveal that BmCREC is indispensable for maintaining ER/Golgi morphology.
From the Key Laboratory of Cell Proliferation and Differentiation of the Ministry of Education and State Key Laboratory of Biomembrane and Membrane Bioengineering, College of Life Sciences, Peking University, Beijing 100871, China.
Qiao
Q
Wang
Birong
B
Shen
Pengli
P
Zheng
Hui
H
Feng
Yige
Y
Guo
Wenyuan
W
Cao
Liang
L
Chen
Xiao
X
Liu
Guodong
G
Zhao
Sizheng
S
Xu
Weide
W
Shen
Jianguo
J
Chen
Junlin
J
Teng
D020816:Amino Acid Motifs; D000818:Animals; D012831:Bombyx; D002467:Cell Nucleus / Q000378:metabolism; D020755:Coat Protein Complex I / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D004278:DNA, Superhelical / Q000737:chemistry / Q000235:genetics*; D004721:Endoplasmic Reticulum / Q000378:metabolism*; D031541:Fluorescence Resonance Energy Transfer; D006056:Golgi Apparatus / Q000378:metabolism*; D019476:Insect Proteins / Q000235:genetics / Q000502:physiology*; D016547:Kinesins / Q000378:metabolism; D017434:Protein Structure, Tertiary; D021381:Protein Transport; D014162:Transfection
D020755:Coat Protein Complex I; D004278:DNA, Superhelical; D019476:Insect Proteins; D016547:Kinesins
BmCREC; COPI; Endoplasmic Reticulum (ER); Golgi; Intracellular Trafficking; KDEL Receptor; Kinesin; PSG Tube Expansion; Vesicles