Mild hypothermia inhibits differentiation of human embryonic and induced pluripotent stem cells.
Culture of pluripotent stem cells at 35°C strikingly reduces unwanted spontaneous differentiation during hESC and iPSC maintenance compared with 37°C. Growth at 35°C did not affect expression of pluripotency mRNAs nor induce expression of cold-inducible genes. Colony size was somewhat reduced at 35°C. Thus, growth at 35°C is a convenient, simple method to reduce the labor of removing spontaneously differentiated colonies when maintaining pluripotent cells.
Department of Neuroscience, University of Connecticut Health Center, Farmington, CT, USA. belinsky@uchc.edu
Glenn S
GS
Belinsky
Srdjan D
SD
Antic
D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation; D002460:Cell Line; D053595:Embryonic Stem Cells / Q000502:physiology*; D005786:Gene Expression Regulation; D006801:Humans; D007035:Hypothermia; D057026:Induced Pluripotent Stem Cells / Q000502:physiology*; D013696:Temperature