In vitro monitoring of the formation of pentamers from the monomer of GST fused HPV 16 L1.
The recombinant GST fusion protein HPV 16 L1 from E. coli was proved to exist as a monomer rather than a pentamer, providing the possibility of real-time monitoring of the pentamer formation in vitro. Time-dependent kinetic studies of the process were performed for the first time by using static light scattering and western blot analysis, where the essential factors were revealed, offering a new biotechnical approach for virus control and/or the development of anti-viral agents.
State Key Laboratory of Supramolecular Structure and Materials, Jilin University, Changchun, 130012, China. yqwu@jlu.edu.cn.
Dong-Dong
DD
Zheng
Dong
D
Pan
Xiao
X
Zha
Yuqing
Y
Wu
Chunlai
C
Jiang
Xianghui
X
Yu
D005982:Glutathione Transferase / Q000737:chemistry / Q000378:metabolism*; D052162:Human papillomavirus 16 / Q000737:chemistry / Q000378:metabolism*; D007700:Kinetics; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D013997:Time Factors
D011994:Recombinant Proteins; D005982:Glutathione Transferase