Ionic liquid-mediated molecularly imprinted solid-phase extraction coupled with gas chromatography-electron capture detector for rapid screening of dicofol in vegetables.
New ionic liquid-mediated molecularly imprinted polymers (IL-MIPs) were prepared by precipitation polymerization using 1-butyl-3-methylimidazolium hexafluorophosphate (BMIM(+)PF6(-)) as the auxiliary solvent, α-chloro-DDT as the dummy template, and they were successfully applied as the sorbents of solid-phase extraction (SPE) for rapid screening of dicofol from cabbage, tomato, and carrot samples. The IL-MIPs were characterized by FTIR, FE-SEM, static adsorption and chromatographic evaluation, and the results revealed that the IL-MIPs had higher adsorption capacity and selectivity to dicofol in aqueous solution than that of ionic liquid-mediated non-imprinted polymers (IL-NIPs) and non-imprinted polymers (NIPs). Under the optimized conditions, the IL-MIPs-SPE-GC method offered good linearity (0.4-40.0ngg(-1), r(2)=0.9995) and the average recoveries of dicofol at three spiked levels were in a range of 84.6-104.1% (n=3) with RSD≤7.6%. The proposed method obviously improved the selectivity and purification effect, and eliminated the effect of template leakage on dicofol quantitative analysis.
Key Laboratory of Medicinal Chemistry and Molecular Diagnosis, Ministry of Education, College of Pharmaceutical Sciences, Hebei University, Baoding 071002, China. yanhy@hbu.edu.cn
Hongyuan
H
Yan
Ning
N
Sun
Yehong
Y
Han
Chen
C
Yang
Mingyu
M
Wang
Ruijun
R
Wu
D000097:Acetonitriles / Q000737:chemistry; D000327:Adsorption; D002849:Chromatography, Gas / Q000379:methods; D004010:Dicofol / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D006863:Hydrogen-Ion Concentration; D007093:Imidazoles / Q000737:chemistry; D052578:Ionic Liquids / Q000737:chemistry*; D054802:Molecular Imprinting / Q000379:methods*; D010573:Pesticide Residues / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D058105:Polymerization; D015203:Reproducibility of Results; D052616:Solid Phase Extraction / Q000379:methods*; D014675:Vegetables / Q000737:chemistry*
C412621:1-butyl-3-methylimidazolium hexafluorophosphate; D000097:Acetonitriles; D007093:Imidazoles; D052578:Ionic Liquids; D010573:Pesticide Residues; D004010:Dicofol; C032159:acetonitrile
Dicofol; Dummy imprinted polymers; Ionic liquid; Solid-phase extraction; Vegetables