Tendon gradient mineralization for tendon to bone interface integration.
Tendon-to-bone integration is a great challenge for tendon or ligament reconstruction regardless of use of autograft or allograft tendons. We mineralized the tendon, thus transforming the tendon-to-bone into a "bone-to-bone" interface for healing. Sixty dog flexor digitorum profundus (FDP) tendons were divided randomly into five groups: (1) normal FDP tendon, (2) CaP (non-extraction and mineralization without fetuin), (3) CaPEXT (Extraction by Na2 HPO4 and mineralization without fetuin), (4) CaPFetuin (non-extraction and mineralization with fetuin), and (5) CaPEXTFetuin (extraction and mineralization with fetuin). The calcium and phosphate content significantly increased in tendons treated with combination of extraction and fetuin compared to the other treatments. Histology also revealed a dense mineral deposition throughout the tendon outer layers and penetrated into the tendon to a depth of 200 µm in a graded manner. Compressive moduli were significantly lower in the four mineralized groups compared with normal control group. No significant differences in maximum failure strength or stiffness were found in the suture pull-out test among all groups. Mineralization of tendon alters the interface from tendon to bone into mineralized tendon to bone, which may facilitate tendon-to-bone junction healing following tendon or ligament reconstruction.
Division of Orthopedic Research, Department of Orthopedic Surgery, Biomechanics & Tendon and Soft Tissue Laboratories, Mayo Clinic, 200 First Street SW, Rochester, Minnesota, 55905.
Jin
J
Qu
Andrew R
AR
Thoreson
Qingshan
Q
Chen
Kai-Nan
KN
An
Peter C
PC
Amadio
Chunfeng
C
Zhao
D064591:Allografts; D000818:Animals; D001696:Biomechanical Phenomena; D001842:Bone and Bones / Q000601:surgery*; D002113:Calcification, Physiologic; D002118:Calcium / Q000032:analysis; D004285:Dogs; D008297:Male; D010710:Phosphates / Q000032:analysis; D019651:Plastic Surgery Procedures / Q000379:methods*; D013710:Tendons / Q000737:chemistry / Q000637:transplantation* / Q000648:ultrastructure; D014945:Wound Healing
D010710:Phosphates; D002118:Calcium
graded mineral; tendon allografts; tendon mineralization; tendon-to-bone healing