Modulatory effects of sesamol in dinitrochlorobenzene-induced inflammatory bowel disorder in albino rats.
BACKGROUND
Inflammatory bowel disease (IBD) is a chronic inflammatory condition of gastrointestinal tract of immune, genetic and environmental origin. In the present study, we examined the effect of sesamol (SES), the main anti-oxidative constituent of Sesamum indicum (sesame seed) Linn. in the dinitrochlorobenzene (DNCB)-induced model for IBD in rats.


METHODS
The groups were divided into normal control, DNCB control, SES and sulfasalazine (SS). On day 24, the rats were killed, colon removed and the macroscopic, biochemical and histopathological evaluations were performed.


RESULTS
The levels of MPO, TBARS and nitrite increased significantly (p < 0.05) in the DNCB group, whereas reduced significantly in the SES, SS treated groups. Serum nitrite levels were found to be insignificant between the different groups. IL-6 and TNF-α levels were significantly high in the DNCB group.


CONCLUSIONS
We conclude the mucosal protective effect of SES on colon due to its potent antioxidant actions. Further investigation is required in a chronic model of different rodent strain for its role involved in the cytokine pathway.
Department of Pharmacology, Manipal College of Pharmaceutical Sciences, Manipal University, Manipal-576104, Karnataka, India.
Phani Krishna
PK
Kondamudi
Hemalatha
H
Kovelamudi
Geetha
G
Mathew
Pawan G
PG
Nayak
C Mallikarjuna
CM
Rao
Rekha R
RR
Shenoy
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D052117:Benzodioxoles / Q000494:pharmacology*; D001835:Body Weight / Q000187:drug effects; D003106:Colon; D004137:Dinitrochlorobenzene / Q000009:adverse effects*; D016179:Granulocyte Colony-Stimulating Factor / Q000378:metabolism; D015212:Inflammatory Bowel Diseases / Q000097:blood / Q000139:chemically induced* / Q000188:drug therapy* / Q000378:metabolism; D007377:Interleukin-3 / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D008297:Male; D009573:Nitrites / Q000097:blood / Q000378:metabolism; D010636:Phenols / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D000975:Antioxidants; D052117:Benzodioxoles; D004137:Dinitrochlorobenzene; D007377:Interleukin-3; D015850:Interleukin-6; D009573:Nitrites; D010636:Phenols; D011993:Recombinant Fusion Proteins; D017392:Thiobarbituric Acid Reactive Substances; D014409:Tumor Necrosis Factor-alpha; C404145:myelopoietin; D016179:Granulocyte Colony-Stimulating Factor; C025583:sesamol