Effects of push/pull perfusion and ultrasonication on the extraction efficiencies of phthalate esters in sports drink samples using on-line hollow-fiber liquid-phase microextraction.
In previous studies, we developed a process, on-line ultrasound-assisted push/pull perfusion hollow-fiber liquid-phase microextraction (UA-PPP-HF-LPME), combining the techniques of push/pull perfusion (PPP) and ultrasonication with hollow-fiber liquid-phase microextraction (HF-LPME), to achieve rapid extraction of acidic phenols from water samples. In this present study, we further evaluated three more-advanced and novel effects of PPP and ultrasonication on the extraction efficiencies of neutral high-molecular-weight phthalate esters (HPAEs) in sports drinks. First, we found that inner-fiber fluid leakage occurs only in push-only perfusion-based and pull-only perfusion-based HF-LPME, but not in the PPP mode. Second, we identified a significant negative interaction between ultrasonication and temperature. Third, we found that the extraction time of the newly proposed system could be shortened by more than 93%. From an investigation of the factors affecting UA-PPP-HF-LPME, we established optimal extraction conditions and achieved acceptable on-line enrichment factors of 92-146 for HPAEs with a sampling time of just 2 min.
Department of Public Health, College of Health Science, Kaohsiung Medical University, Kaohsiung 886-7, Taiwan.
Yu-Ying
YY
Chao
Chien-Hung
CH
Lee
Tzu-Yang
TY
Chien
Yu-Hsuan
YH
Shih
Yin-An
YA
Lu
Ting-Hsuan
TH
Kuo
Yeou-Lih
YL
Huang
D001628:Beverages / Q000032:analysis*; D004952:Esters / Q000737:chemistry / Q000302:isolation & purification*; D059627:Liquid Phase Microextraction / Q000295:instrumentation / Q000379:methods*; D008970:Molecular Weight; D010795:Phthalic Acids / Q000737:chemistry / Q000302:isolation & purification*; D013010:Sonication / Q000379:methods*
D004952:Esters; D010795:Phthalic Acids; C032279:phthalic acid