[A method for the primary culture of fibroblasts isolated from human airway granulation tissues].
OBJECTIVE
To establish a feasible method to culture primary fibroblasts isolated from human airway granulation tissues, and therefore to provide experimental data for the investigation of the pathogenesis of benign airway stenosis.


METHODS
The granulation tissues were collected from 6 patients during routine bronchoscopy at our department of Beijing Tiantan Hospital from April to June 2011. Primary fibroblasts were obtained by culturing the explanted tissues. Cell growth was observed under inverted microscope.


RESULTS
All of these 6 primary cultures were successful. Fibroblast-like cells were observed to migrate from the tissue pieces 3 d after inoculation. After 9-11 d of culture, cells reached to 90% confluence and could be sub-cultured. After passage, the cells were still in a typical elongated spindle-shape and grew well. The cells could be sub-cultured further when they formed a monolayer.


CONCLUSIONS
Explant culture is a reliable method for culturing primary fibroblasts from human airway granulation tissues.
Department of Pulmonary Medicine, Beijing Tiantan Hospital, Capital Medical University, Beijing 100050, China.
Nan
N
Chen
Jie
J
Zhang
Min
M
Xu
Yu-ling
YL
Wang
Ying-hua
YH
Pei
D001980:Bronchi / Q000166:cytology; D018929:Cell Culture Techniques / Q000379:methods*; D049109:Cell Proliferation; D002469:Cell Separation / Q000379:methods; D002478:Cells, Cultured; D003470:Culture Media; D005260:Female; D005347:Fibroblasts / Q000166:cytology* / Q000648:ultrastructure; D006097:Granulation Tissue / Q000166:cytology*; D006801:Humans; D008297:Male; D014135:Tracheal Stenosis / Q000473:pathology*
D003470:Culture Media