Association of MTHFR C677T polymorphism with bone mineral density of osteoporosis in postmenopausal Thai women.
BACKGROUND
Osteoporosis and osteopenia is rising with the increase in numbers of postmenopausal women. Methylenetetrahydrofolate reductase (MTHFR), a homocysteine catabolizing enzyme, is involved in the regulation of bone mineral density (BMD). The association between MTHFR C677T polymorphism with osteoporosis in postmenopausal Thai women is hitherto unclear.


OBJECTIVE
To investigate the association between MTHFR C677T and BMD in postmenopausal Thai women.


METHODS
The study subjects consisted of 346 postmenopausal Thai women volunteers. Standard dual-energy X-ray absorptiometry (DXA) was used for measurement of BMD T-score. Restriction fragment length polymorphism (RFLP) analysis was used for measurement of MTHFR C677T polymorphism.


RESULTS
In the evaluation of 346 postmenopausal Thai women heterozygous (CT) genotype had a risk of osteopenia than normal control (odds ratio (OR) = 5.66, p < 0.001). BMD T-scores at each bone position revealed that heterozygous (CT) genotype had increased risk of osteopenic bones than normal controls at lumbar spines 1, 2, and 4 (OR = 2.48, p < 0.001, OR = 1.98, p = 0.008 and OR = 1.83, p = 0.016 respectively), ward's triangle (OR = 2.08, p = 0.008), and head of radius (OR = 2.95, p = 0.008).


CONCLUSIONS
These results indicate the possibility of using MTHFR C677T polymorphism to identify postmenopausal Thai women at high risk of osteopenia.
Department of Tropical Nutrition and Food Science, Faculty of Tropical Medicine, Mahidol University Bangkok, Thailand.
Chutaporn
C
Tongboonchoo
Anchalee
A
Tungtrongchitr
Benjaluck
B
Phonrat
Sangchai
S
Preutthipan
Rungsunn
R
Tungtrongchitr
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015519:Bone Density / Q000502:physiology; D001851:Bone Diseases, Metabolic / Q000235:genetics; D005260:Female; D006801:Humans; D042965:Methylenetetrahydrofolate Reductase (NADPH2) / Q000235:genetics*; D015663:Osteoporosis, Postmenopausal / Q000235:genetics*; D010641:Phenotype; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D013785:Thailand
C573423:MTHFR protein, human; D042965:Methylenetetrahydrofolate Reductase (NADPH2)