Technical considerations for analyzing EMT-MET data from surgical samples.
With an increase of neoadjuvant therapy, biopsy samples have become imperative for cancer research; however, what kind of difference between surgical and endoscopic biopsy samples in gene expression profiles was still unclear. Recently, we reported artificially induced epithelial-mesenchymal transition (aiEMT) in the surgical samples by comparison between gene expression profiles of both samples of the esophagus. This was also found in mouse epithelium under an ischemic condition for 4h. This study will evoke underestimation of the prognostic evaluation power of EMT related markers in past cancer research and prevalence of biopsy samples for in vivo expression profiling with low biases.
Division of Genetics, National Cancer Center Research Institute, Chuo-ku, Tokyo 104-0045, Japan.
Kazuhiko
K
Aoyagi
Masashi
M
Tamaoki
Takao
T
Nishumura
Hiroki
H
Sasaki
D000818:Animals; D001706:Biopsy / Q000379:methods*; D058750:Epithelial-Mesenchymal Transition; D004947:Esophagus / Q000473:pathology; D020869:Gene Expression Profiling / Q000379:methods*; D018398:Homeodomain Proteins / Q000235:genetics; D006801:Humans; D007511:Ischemia / Q000235:genetics; D051379:Mice; D009369:Neoplasms / Q000235:genetics / Q000473:pathology / Q000601:surgery*; D009687:Nuclear Proteins / Q000235:genetics; D012097:Repressor Proteins / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics; D051879:Twist-Related Protein 1 / Q000235:genetics; D000074165:Zinc Finger E-box Binding Homeobox 2; D000071799:Zinc Finger E-box-Binding Homeobox 1
D018398:Homeodomain Proteins; D009687:Nuclear Proteins; D012097:Repressor Proteins; C103173:TWIST1 protein, human; D014157:Transcription Factors; D051879:Twist-Related Protein 1; C486307:ZEB1 protein, human; C492363:ZEB2 protein, human; D000074165:Zinc Finger E-box Binding Homeobox 2; D000071799:Zinc Finger E-box-Binding Homeobox 1
Artificially induced EMT; Esophageal cancer; Expression profile; Surgical sample