Labeling Cytosolic Targets in Live Cells with Blinking Probes.
With the advent of superresolution imaging methods, fast dynamic imaging of biological processes in live cells remains a challenge. A subset of these methods requires the cellular targets to be labeled with spontaneously blinking probes. The delivery and specific targeting of cytosolic targets and the control of the probes' blinking properties are reviewed for three types of blinking probes: quantum dots, synthetic dyes, and fluorescent proteins.
Department of Chemistry & Biochemistry, University of California Los Angeles, Los Angeles CA 90095.
Jianmin
J
Xu
Jason
J
Chang
Qi
Q
Yan
Thomas
T
Dertinger
Marcel
M
Bruchez
Shimon
S
Weiss
Fluorescent proteins; Optical nanoscopy; Quantum dots; Single molecule localization microscopy; Superresolution imaging; Synthetic dyes