Kinetic study of a novel thermo-stable α-carbonic anhydrase for biomimetic CO2 capture.
Biomimetic CO2 capture includes environmentally friendly solutions based on carbonic anhydrase (CA), an enzyme that increases CO2 absorption rate in conventional acid-gas scrubbing processes. The present contribution reports the characterization of a new recombinant carbonic anhydrase, SspCA, isolated from the thermophile bacterium Sulphurhydrogenibium yellowstonense sp. YO3AOP1. The kinetics of SspCA was characterized in terms of first order CO2 hydration rate according to a procedure based on CO2 absorption tests in a stirred cell apparatus. The first order kinetic constant at 25°C was 9.16 × 10(6) L/(mols). An appropriate investigation on SspCA stability was carried out to assess its long-term resistance to high temperatures as in all capture processes based on absorption/vacuum-desorption cycles. Its half-life was 53 and 8 days at 40 °C and 70 °C, respectively.
Istituto di Ricerche sulla Combustione - Consiglio Nazionale delle Ricerche, Napoli, Italy.
Maria Elena
ME
Russo
Giuseppe
G
Olivieri
Clemente
C
Capasso
Viviana
V
De Luca
Antonio
A
Marzocchella
Piero
P
Salatino
Mosè
M
Rossi
D001419:Bacteria / Q000201:enzymology / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D032701:Biomimetics / Q000379:methods; D002245:Carbon Dioxide / Q000378:metabolism*; D002256:Carbonic Anhydrases / Q000235:genetics / Q000378:metabolism*; D004795:Enzyme Stability; D006358:Hot Temperature; D007700:Kinetics; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism
D001426:Bacterial Proteins; D011994:Recombinant Proteins; D002245:Carbon Dioxide; D002256:Carbonic Anhydrases
Absorption; CO(2) capture; Carbonic anhydrase; Thermo-stable enzyme