The force of transporting a single amino acid into the living cell measured using atomic force microscopy.
We used single molecule force spectroscopy (SMFS) to investigate the interacting force between single cysteine and amino acid transporters in eukaryotic cell membranes. We measured the transporting force of cysteine and found that its conformation on the AFM tip is important for discriminating the substrate in the transporting pathway.
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, PR China.
Xin
X
Shang
Yuping
Y
Shan
Yangang
Y
Pan
Mingjun
M
Cai
Junguang
J
Jiang
Hongda
H
Wang
D000596:Amino Acids / Q000378:metabolism*; D002470:Cell Survival; D005057:Eukaryotic Cells / Q000378:metabolism* / Q000648:ultrastructure*; D006367:HeLa Cells; D006801:Humans; D018625:Microscopy, Atomic Force; D015394:Molecular Structure
D000596:Amino Acids