Sequential formation of regioselective disulfide bonds in synthetic peptides with multiple disulfide bonds.
Numerous methods have been developed for the formation of disulfide bonds in recombinant DNA-derived and chemically synthesized peptides and proteins, but only a few have found widespread acceptance. The choice of method(s) for formation of disulfide in synthetic peptides and proteins needs to be tailored for each individual polypeptide in such a way so that the reaction conditions are selective, efficient, and safe and give the maximum yield. Here we describe the sequential formation of three disulfide bonds regioselectively which has been optimized for the synthesis of two-chained, heterodimeric polypeptide members of the insulin-relaxin superfamily.
Florey Neuroscience Institutes, School of Chemistry, The University of Melbourne, Parkville, VIC, Australia.
Fazel
F
Shabanpoor
Mohammed Akhter
MA
Hossain
Feng
F
Lin
John D
JD
Wade
D000596:Amino Acids / Q000737:chemistry; D004220:Disulfides / Q000737:chemistry*; D010455:Peptides / Q000138:chemical synthesis* / Q000737:chemistry
D000596:Amino Acids; D004220:Disulfides; D010455:Peptides