Grape polyphenols increase the activity of HDL enzymes in old and obese rats.
HDL particles are protein-rich particles that act as a vehicle for reverse cholesterol transport from tissues to the liver. The purpose of this study was to investigate age-dependent changes in the functional activity of HDL and the effect of high-energy diet on this index, as well as to correct it under the influence of grape polyphenols from "Enoant" obtained from Vitis vinifera grapes. We observed the age-dependent composition changes in HDL particle. It was shown that total lipids and triacylglycerol (TG) levels were higher in 24-month-old animals. In obese rats, HDL total lipids and TG levels were higher in 24-month-old than in the 3-month-old and 12-month-old groups but did not differ from 24-month-old group. The plasma HDL paraoxonase (PON) and lecithin:cholesterol acyltransferase (LCAT) activity levels were decreased in old-aged rats, and cholesteryl ester transfer protein (CETP) activity was higher in old rats. Keeping 12-month-old animals on high-fructose diet completely leveled the age differences in the data that have been measured between 12-month-old and 24-month-old rats. After "Enoant" administration, an increase of HDL PON and LCAT activity levels and a reduction of CETP activity were found in 24-month-old and obese rats.
Biochemistry Department, National University of Pharmacy, Melnikova street, 12, Kharkiv 61002, Ukraine. andrey.zagayko@gmail.com
Andriy L
AL
Zagayko
Ganna B
GB
Kravchenko
Oksana A
OA
Krasilnikova
Yuri O
YO
Ogai
D000818:Animals; D043303:Aryldialkylphosphatase / Q000097:blood / Q000378:metabolism*; D053480:Cholesterol Ester Transfer Proteins / Q000097:blood / Q000378:metabolism*; D008075:Lipoproteins, HDL / Q000097:blood; D008297:Male; D059808:Polyphenols / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D014280:Triglycerides / Q000097:blood; D027843:Vitis / Q000737:chemistry*
D053480:Cholesterol Ester Transfer Proteins; D008075:Lipoproteins, HDL; D059808:Polyphenols; D014280:Triglycerides; D043303:Aryldialkylphosphatase