Protective effect of detoxified Rhus verniciflua stokes on human keratinocytes and dermal fibroblasts against oxidative stress and identification of the bioactive phenolics.
Oxidative stress due to the over-production of reactive oxygen species (ROS) is associated with human skin aging. This study was designed to identify the bioactive phenolics in detoxified Rhus verniciflua Stokes (DRVS) that may protect human skin against oxidative stress. Under oxidative stress caused by H₂O₂, the 40% (v/v) aqueous methanol extract of DRVS protected human keratinocytes in a dose-dependent manner. The expression of matrix metalloproteinase-1 (MMP-1) was also inhibited by the DRVS extract in human dermal fibroblasts-neonatal cells exposed to ultraviolet A. The major bioactive phenolics of DRVS were tentatively identified by LC/Q-TOF-ESI-MS/MS, and included gallic acid, 2-(ethoxymethoxy)-3-hydroxyphenol, fustin, a fustin isomer, tetragalloyl glucose, pentagalloyl glucose, fisetin, sulfuretin, a sulfuretin isomer, and butein. The results suggest that a DRVS extract may be effective in slowing skin aging through its antioxidative properties and by down-regulating MMP-1 expression. Further studies are needed to examine whether this effect would be mediated by the phenolics identified in this study.
Department of Food Science and Biotechnology, Kyung Hee University.
Chun-Shan
CS
Liu
Tae-Gyu
TG
Nam
Min-Woo
MW
Han
Soo-mi
SM
Ahn
Han Seok
HS
Choi
Tae Young
TY
Kim
Ock K
OK
Chun
Sung I
SI
Koo
Dae-Ok
DO
Kim
D002460:Cell Line; D005347:Fibroblasts / Q000187:drug effects* / Q000528:radiation effects; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D006861:Hydrogen Peroxide / Q000633:toxicity; D015603:Keratinocytes / Q000187:drug effects* / Q000528:radiation effects; D020781:Matrix Metalloproteinase 1 / Q000096:biosynthesis; D018384:Oxidative Stress / Q000187:drug effects / Q000528:radiation effects; D010636:Phenols / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D017382:Reactive Oxygen Species; D053719:Tandem Mass Spectrometry; D012251:Toxicodendron / Q000737:chemistry*; D014466:Ultraviolet Rays
D010636:Phenols; D010936:Plant Extracts; D017382:Reactive Oxygen Species; D006861:Hydrogen Peroxide; D020781:Matrix Metalloproteinase 1