Occurrence of trypsin-like protease in cardamom (Elettaria cardamomum Maton).
Occurrence of trypsin-like protease in fresh cardamom (Elettaria cardamomum Maton) seeds, as evidenced by the benzoyl-arg-p-nitroanilide (BApNA) hydrolyzing ability of the seed enzyme preparation under alkaline condition is reported for the first time. The enzyme has a pH and temperature optima as 8 and 45 degrees C, respectively. It is inhibited by aprotinin and phenylmethyl sulfonyl fluoride (PMSF) in a dose-dependent manner, suggesting the presence of serine residues at the active site. The enzyme had a V(max) of 98.01 nmoles p-nitroaniline released per min per mg protein and K(m) of 0.0684 mM with BApNA as substrate. Addition of aprotinin (75.75 microM) increased K(m) value by three-folds, whereas the V(max) was reduced by 23%.
Cardamom Research Station, Pampadumpara 685 556, Idukki district, Kerala, India. entojoe2003@yahoo.co.in
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Josephrajkumar
Romit
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Chakrabarty
George
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Thomas
D000814:Aniline Compounds / Q000378:metabolism; D007611:Aprotinin / Q000494:pharmacology; D028523:Elettaria / Q000187:drug effects / Q000201:enzymology*; D010664:Phenylmethylsulfonyl Fluoride / Q000494:pharmacology; D012639:Seeds / Q000187:drug effects / Q000201:enzymology*; D013379:Substrate Specificity; D014357:Trypsin / Q000737:chemistry / Q000378:metabolism*
D000814:Aniline Compounds; C008625:nitroaniline; D010664:Phenylmethylsulfonyl Fluoride; D007611:Aprotinin; D014357:Trypsin