Peimine impairs pro-inflammatory cytokine secretion through the inhibition of the activation of NF-κB and MAPK in LPS-induced RAW264.7 macrophages.
In the previous study, we found that peimine has good anti-inflammatory effects in vivo. However, the anti-inflammatory mechanism of peimine remains unclear. We, therefore, assessed the effects of peimine on inflammatory cytokines in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages. We found that peimine (0-25 mg/L) significantly inhibited tumor necrosis factor (TNF)-α, interleukin (IL)-6, IL-1β, and increased IL-10 production. Furthermore, peimine significantly inhibited the phosphorylation of p38, ERK and c-jun N-terminal kinase (JNK) as well as decreased p65 and IκB. The present results indicate that peimine inhibits the production of inflammatory cytokines induced by LPS through blocking MAPKs and NF-κB signaling pathways.
Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Jilin University , Changchun, Jilin , China .
Peng-Fei
PF
Yi
Yi-Chun
YC
Wu
Hai-Bing
HB
Dong
Yang
Y
Guo
Qian
Q
Wei
Cui
C
Zhang
Zhou
Z
Song
Qian-Qian
QQ
Qin
Shuang
S
Lv
Shuai-Cheng
SC
Wu
Ben-Dong
BD
Fu
D000818:Animals; D002460:Cell Line; D002595:Cevanes / Q000494:pharmacology*; D016207:Cytokines / Q000276:immunology* / Q000378:metabolism; D048049:Extracellular Signal-Regulated MAP Kinases / Q000276:immunology; D051550:I-kappa B Kinase / Q000276:immunology; D008070:Lipopolysaccharides / Q000633:toxicity; D020935:MAP Kinase Signaling System / Q000187:drug effects* / Q000276:immunology; D008264:Macrophages / Q000276:immunology* / Q000378:metabolism; D051379:Mice; D051996:Transcription Factor RelA / Q000276:immunology*
D002595:Cevanes; D016207:Cytokines; D008070:Lipopolysaccharides; C498144:Rela protein, mouse; D051996:Transcription Factor RelA; C014242:verticine; D051550:I-kappa B Kinase; D048049:Extracellular Signal-Regulated MAP Kinases