In vivo ozone exposure does not increase DNA single-strand breaks in human peripheral lymphocytes.
In this randomized parallel study, we examined whether an acute ozone (O3) exposure leads to increased DNA strand breaks in human lymphocytes. The groups were exposed to 0.21 ppm O3 or filtered air for two hours. 30min and 4.5 h after exposure, DNA damage was determined in isolated lymphocytes using the Fast Micromethod. There was no detectable effect after O3 exposure. We conclude that an acute O3 exposure at the tested concentration does not lead to persistent DNA damage.
1Institute for Physiological Chemistry, University Medical Center, Johannes Gutenberg University Mainz, Mainz, Germany.
C
C
Finkenwirth
B
B
Roßbach
H C
HC
Schröder
A
A
Muttray
D004249:DNA Damage; D004277:DNA, Single-Stranded / Q000187:drug effects*; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects* / Q000378:metabolism; D010126:Ozone / Q000633:toxicity*
D004277:DNA, Single-Stranded; D010126:Ozone
DNA single-strand breaks; Fast Micromethod; Ozone; peripheral blood lymphocytes