ABA-insensitive (ABI) 4 and ABI5 synergistically regulate DGAT1 expression in Arabidopsis seedlings under stress.
Triacylglycerol (TAG) accumulation is essential for seed maturation in plants. Diacylglycerol acyltransferase 1 (DGAT1) is the rate-limiting enzyme in TAG biosynthesis. In this study, we show that TAG accumulation in Arabidopsis seedlings is correlated with environmental stress, and both ABI4 and ABI5 play important roles in regulating DGAT1 expression. Tobacco transient assays revealed the synergistic effect of ABI4 with ABI5 in regulating DGAT1 expression. Taken together, our findings indicate ABI5 is an important accessory factor with ABI4 in the activation of DGAT1 in Arabidopsis seedlings under stress.
State Key Laboratory of Protein and Plant Gene Research, College of Life Sciences, Peking University, Beijing 100871, People's Republic of China.
Yinfei
Y
Kong
Songbin
S
Chen
Yang
Y
Yang
Chengcai
C
An
D000040:Abscisic Acid / Q000494:pharmacology; D016960:Agrobacterium tumefaciens / Q000235:genetics / Q000378:metabolism; D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D050976:Basic-Leucine Zipper Transcription Factors / Q000235:genetics* / Q000378:metabolism; D051048:Diacylglycerol O-Acyltransferase / Q000235:genetics* / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D017930:Genes, Reporter; D018525:Germination / Q000187:drug effects; D008156:Luciferases; D010937:Plant Growth Regulators / Q000494:pharmacology; D030821:Plants, Genetically Modified / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D036226:Seedlings / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D013312:Stress, Physiological; D014026:Nicotiana / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism; D014280:Triglycerides / Q000096:biosynthesis
C470421:ABI4 protein, Arabidopsis; C409743:ABI5 protein, Arabidopsis; D029681:Arabidopsis Proteins; D050976:Basic-Leucine Zipper Transcription Factors; D010937:Plant Growth Regulators; D014157:Transcription Factors; D014280:Triglycerides; D000040:Abscisic Acid; D008156:Luciferases; C579477:DGAT1 protein, Arabidopsis; D051048:Diacylglycerol O-Acyltransferase
ABI4; ABI5; DGAT1; Synergistic effect; Triacylglycerol