In-depth proteomic analysis of mouse microglia using a combination of FASP and StageTip-based, high pH, reversed-phase fractionation.
Microglia are major immune cells in the central nervous system. A characterization of microglia proteome would facilitate on the study of microglial functions in association with various neurodegenerative diseases. To build a reference proteome, we established a BV-2 microglial proteome to a depth of 5494 unique protein groups using a novel strategy that combined FASP, StageTip-based high pH fractionation, and high-resolution MS quickly and cost efficiently. By bioinformatics analysis, the BV-2 proteome is a valuable resource for studies of microglial function, such as in the immune response, inflammatory response, and phagocytosis. All MS data have been deposited in the ProteomeXchange with identifier PXD000168.
Department of Biomedical Engineering, Seoul National University College of Medicine, Seoul, Korea; Institute of Medical & Biological Engineering, Medical Research Center, Seoul National University College of Medicine, Seoul, Korea.
Dohyun
D
Han
Sungyoon
S
Moon
Yikwon
Y
Kim
Jihye
J
Kim
Jonghwa
J
Jin
Youngsoo
Y
Kim
D053000:Analytic Sample Preparation Methods; D000818:Animals; D002460:Cell Line; D005591:Chemical Fractionation / Q000379:methods*; D002853:Chromatography, Liquid; D056148:Chromatography, Reverse-Phase / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D051379:Mice; D017628:Microglia / Q000378:metabolism*; D020543:Proteome / Q000378:metabolism; D040901:Proteomics / Q000379:methods*; D015398:Signal Transduction
D020543:Proteome
Cell biology; High pH reversed-phase fractionation; Microglia; Protein profile; Technology