[Effect of hypoxia on gastric cancer cell proliferation and LINE-1 endonuclease variant GCRG213 expression].
OBJECTIVE
To investigate the change of gastric cancer cell proliferation and the expression of gastric cancer related gene 213 (GCRG213), a long interspersed nuclear element-1 (LINE-1) endonuclease variant, during hypoxia.


METHODS
Normal gastric mucosa cell GES-1 and gastric cancer cell BGC-823 were cultured in 20% or 3% oxygen concentrations, respectively. MTT test was used to analyze the proliferation of the GES-1 and BGC-823 cells. The change of GCRG213 mRNA and protein expression in GES-1 and BGC-823 cells was detected by using RT-PCR and Western blot analysis. Blast was used at the NCBI Blast server to identify GCRG213 sequence to any alignment in the GeneBank databases.


RESULTS
Compared with 20% oxygen condition, 3% oxygen concentration could promote cell growth. Mean-while, the expression of GCRG213 at mRNA and protein levels was increased. GCRG213 sequence shared high homology with LINE-1 endonuclease sequence.


CONCLUSIONS
GCRG213 is a variant of LINE-1 endonuclease. Hypoxia as in 3% oxygen condition can promote cell proliferation and lead to GCRG213 overexpression.
Department of Geriatric Gastroenterology, China PLA General Hospital, Beijing 100853, China.
Yu
Y
Tian
Xin
X
Huang
Tong
T
Zhao
Ling-Ling
LL
Zhu
Gang-Shi
GS
Wang
D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002478:Cells, Cultured; D003850:Deoxyribonuclease I / Q000235:genetics*; D005753:Gastric Mucosa / Q000166:cytology; D015870:Gene Expression; D006801:Humans; D000860:Hypoxia; D036361:Peptide Hormones / Q000235:genetics*; D013274:Stomach Neoplasms / Q000235:genetics* / Q000473:pathology*
C477000:GKN1 protein, human; D036361:Peptide Hormones; D003850:Deoxyribonuclease I; C538849:LINE-1 endonuclease, human