Femtosecond laser-induced micropattern and Ca/P deposition on Ti implant surface and its acceleration on early osseointegration.
Surface microstructure and chemical composition of the implant are very important for its osseointegration in vivo. In this paper, a hierarchical micropattern covered with calcium phosphate (Ca/P phase) was obtained on titanium (Ti) implant surface by femtosecond lasers (FSL) irradiation in hydroxyapatite suspension. The hierachical micropattern as well as Ca/P phase increased osteoblastic cell adhesion. Higher expression of osteogenic markers (osteocalcin, osteopontin, and runt related transcription factor-2) on the surface treated by FSL of 2.55 J/cm(2) indicated the favorable effect of laser treatment on cell differentiation. In vivo studies were carried out to evaluate the effect of laser treatment and Ca/P deposition on the osseointegration. It showed that the binding capacity between bone and FSL-treated Ti implants was obviously stronger than that between bone and polished or sand blasting and acid etching (SLA) Ti implants. Bone trabecula surrounded the FSL-treated implants without fibrous tissue after 8-week implantation. Also, higher bone mineral density was seen surrounding the FSL-treated implants. Our in vitro and in vivo studies demonstrated that the FSL induced micropattern and Ca/P phase had positive effects on the acceleration of early osseointegration of Ti implants with bone tissue.
School of Materials Science and Engineering, Hebei University of Technology, Tianjin 300130, China. Liangchunyong@126.com
Chunyong
C
Liang
Hongshui
H
Wang
Jianjun
J
Yang
Yanli
Y
Cai
Xin
X
Hu
Yang
Y
Yang
Baoe
B
Li
Hongjie
H
Li
Haipeng
H
Li
Changyi
C
Li
Xianjin
X
Yang
D015519:Bone Density; D001842:Bone and Bones / Q000293:injuries; D002130:Calcium Phosphates / Q000737:chemistry; D020099:Coated Materials, Biocompatible; D017886:Durapatite / Q000737:chemistry; D007834:Lasers; D016348:Osseointegration; D019736:Prostheses and Implants; D013499:Surface Properties / Q000528:radiation effects; D014025:Titanium / Q000737:chemistry*
D002130:Calcium Phosphates; D020099:Coated Materials, Biocompatible; D017886:Durapatite; D014025:Titanium