Enhanced nanomagnetic gene transfection of human prenatal cardiac progenitor cells and adult cardiomyocytes.
Magnetic nanoparticle-based gene transfection has been shown to be an effective, non-viral technique for delivery of both plasmid DNA and siRNA into cells in culture. It has several advantages over other non-viral delivery techniques, such as short transfection times and high cell viability. These advantages have been demonstrated in a number of primary cells and cell lines. Here we report that oscillating magnet array-based nanomagnetic transfection significantly improves transfection efficiency in both human prenatal cardiac progenitor cells and adult cardiomyocytes when compared to static magnetofection, cationic lipid reagents and electroporation, while maintaining high cell viability. In addition, transfection of adult cardiomyocytes was improved further by seeding the cells onto Collagen I-coated plates, with transfection efficiencies of up to 49% compared to 24% with lipid reagents and 19% with electroporation. These results demonstrate that oscillating nanomagnetic transfection far outperforms other non-viral transfection techniques in these important cells.
nanoTherics Limited, Keele University Science and Business Park, Newcastle under Lyme, Staffordshire, United Kingdom.
Mahendran
M
Subramanian
Jenson
J
Lim
Jon
J
Dobson
D002448:Cell Adhesion / Q000235:genetics; D002470:Cell Survival / Q000235:genetics; D002478:Cells, Cultured; D004247:DNA / Q000737:chemistry / Q000235:genetics*; D018274:Electroporation / Q000379:methods; D004622:Embryo, Mammalian / Q000166:cytology; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D008055:Lipids / Q000737:chemistry; D008280:Magnetics; D008856:Microscopy, Fluorescence; D032383:Myocytes, Cardiac / Q000166:cytology / Q000378:metabolism*; D053758:Nanoparticles / Q000737:chemistry; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism*; D014162:Transfection / Q000379:methods*
D008055:Lipids; D049452:Green Fluorescent Proteins; D004247:DNA