Fluorescent dye cocktail for multiplex drug-site mapping on human serum albumin.
Elucidating how molecules bind to HSA is fundamental for predicting drug incompatibilities. Through combinatorial screening, we identified a novel fluorescent dye (BD140) with turn-on fluorescence emission and specific binding at HSA drug site 2. We further combined it with dansylamide to develop a fluorescent dye cocktail for high-throughput mapping of the interaction between therapeutics at HSA drug-binding sites.
Department of Chemistry, National University of Singapore , 3 Science Drive 2, 117543 Singapore.
Jun Cheng
JC
Er
Marc
M
Vendrell
Mui Kee
MK
Tang
Duanting
D
Zhai
Young-Tae
YT
Chang
D001665:Binding Sites; D005456:Fluorescent Dyes / Q000737:chemistry*; D057166:High-Throughput Screening Assays; D006801:Humans; D008958:Models, Molecular; D015394:Molecular Structure; D004364:Pharmaceutical Preparations / Q000737:chemistry*; D012709:Serum Albumin / Q000737:chemistry*
D005456:Fluorescent Dyes; D004364:Pharmaceutical Preparations; D012709:Serum Albumin