Improvement of solvent production from xylose mother liquor by engineering the xylose metabolic pathway in Clostridium acetobutylicum EA 2018.
Xylose mother liquor (XML) is a by-product of xylose production through acid hydrolysis from corncobs, which can be used potentially for alternative fermentation feedstock. Sixteen Clostridia including 13 wild-type, 1 industrial strain, and 2 genetically engineered strains were screened in XML, among which the industrial strain Clostridium acetobutylicum EA 2018 showed the highest titer of solvents (12.7 g/L) among non-genetic populations, whereas only 40% of the xylose was consumed. An engineered strain (2018glcG-TBA) obtained by combination of glcG disruption and expression of the D-xylose proton-symporter, D-xylose isomerase, and xylulokinase was able to completely utilize glucose and L-arabinose, and 88% xylose in XML. The 2018glcG-TBA produced total solvents up to 21 g/L with a 50% enhancement of total solvent yield (0.33 g/g sugar) compared to that of EA 2018 (0.21 g/g sugar) in XML. This XML-based acetone-butanol-ethanol fermentation using recombinant 2018glcG-TBA was estimated to be economically promising for future production of solvents.
Key Laboratory of Synthetic Biology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, 200032, China.
Zhilin
Z
Li
Han
H
Xiao
Weihong
W
Jiang
Yu
Y
Jiang
Sheng
S
Yang
D019747:Aldose-Ketose Isomerases / Q000378:metabolism; D000440:Butanols / Q000378:metabolism*; D046969:Clostridium acetobutylicum / Q000235:genetics* / Q000378:metabolism*; D005285:Fermentation; D005818:Genetic Engineering; D005947:Glucose / Q000378:metabolism; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000378:metabolism; D012997:Solvents / Q000378:metabolism*; D014994:Xylose / Q000378:metabolism*; D003313:Zea mays / Q000737:chemistry
D000440:Butanols; D012997:Solvents; D014994:Xylose; D017853:Phosphotransferases (Alcohol Group Acceptor); C032700:xylulokinase; D019747:Aldose-Ketose Isomerases; C023298:xylose isomerase; D005947:Glucose