[Expression of circulating microRNAs in patients with bladder urothelial carcinoma].
OBJECTIVE
To search for differentially expressed microRNAs in circulation and to explore their potential application as non-invasive biomarkers for bladder urothelial carcinoma.


METHODS
Six bladder urothelial carcinoma patients were recruited into this study, and blood from seven non-tumor patients was included as the controls. Total small RNAs were isolated from the blood. By using high-throughput sequencing technologies, we provided microRNA expression profiles of bladder urothelial carcinoma patients and the control group. The data were analyzed using T test of the SPSS17.0 software to study the expression differences of microRNAs between the two groups.


RESULTS
The work identified some microRNAs with differential expression in circulation between the bladder urothelial carcinoma patients and the non-tumor patients. Five microRNAs (hsa-miR-378g, hsa-miR-942, hsa-miR-106a-5p, hsa-miR-142-3p and hsa-miR-374a) were identified to be upregulated in the patients, and the expressions of many other microRNAs were significantly downregulated.


CONCLUSIONS
The study reveals that there is a variance of microRNA expression profile in circulation between bladder urothelial and non neoplastic populations,The results need further study by large samples.
Department of Urology, Peking University Third Hospital, Beijing 100191, China.
Zhi-cao
ZC
Zhang
Yi
Y
Huang
Xiu-jie
XJ
Wang
Meng
M
Wang
Lu-lin
LL
Ma
D002295:Carcinoma, Transitional Cell / Q000235:genetics*; D015536:Down-Regulation; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D059467:Transcriptome; D015854:Up-Regulation; D001749:Urinary Bladder Neoplasms / Q000235:genetics*
D035683:MicroRNAs