Dectin-2 is predominately macrophage restricted and exhibits conspicuous expression during Aspergillus fumigatus invasion in human lung.
We investigated the features of Dectin-2 expression both at transcriptional and translational levels during Aspergillus fumigatus infection in human lung. Simultaneously, the expression of CD206 was assayed as an activated marker of alveolar macrophages. The characteristic of Dectin-2 expression were then confirmed in Monocyte-derived macrophages (MDM) after A. fumigatus stimulation by Flow Cytometry. We found that the expression of Dectin-2 was low in normal lung, while it revealed a markedly up-regulation during A. fumigatus invasion. Dectin-2 expression was predominantly restricted to CD206 positive cells. There was salient positive correlation between Dectin-2 expression and CD206. We conclude that Dectin-2 expression is largely restricted to alveolar macrophages in human lung. The conspicuous expression of Dectin-2 during A. fumigatus invasion suggests its notable contribution to antifungal defenses in pulmonary aspergillosis.
Department of Respiratory and Critical Care Medicine, Jinling Hospital, Clinical School of Nanjing, Second Military Medical University, Nanjing 210002, China.
He
H
Sun
Xiao-Yong
XY
Xu
Hong-Tao
HT
Shao
Xin
X
Su
Xiao-Dong
XD
Wu
Quan
Q
Wang
Yi
Y
Shi
D000328:Adult; D000368:Aged; D001232:Aspergillus fumigatus / Q000235:genetics / Q000276:immunology*; D015153:Blotting, Western; D005260:Female; D005434:Flow Cytometry; D015966:Gene Expression Regulation, Fungal / Q000276:immunology*; D006801:Humans; D007150:Immunohistochemistry; D037181:Lectins, C-Type / Q000235:genetics / Q000276:immunology*; D016676:Macrophages, Alveolar / Q000276:immunology* / Q000382:microbiology; D008297:Male; D008875:Middle Aged; D055732:Pulmonary Aspergillosis / Q000276:immunology* / Q000382:microbiology; D012331:RNA, Fungal / Q000737:chemistry / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D018709:Statistics, Nonparametric
D037181:Lectins, C-Type; D012331:RNA, Fungal; C445340:dectin-2, mouse
Aspergillus fumigatus; Dectin-2; Mannose receptor; Monocyte-derived macrophage