Deciphering the metabolic pathways influencing heat and cold responses during post-harvest physiology of peach fruit.
Peaches are highly perishable and deteriorate quickly at ambient temperature. Cold storage is commonly used to prevent fruit decay; however, it affects fruit quality causing physiological disorders collectively termed 'chilling injury' (CI). To prevent or ameliorate CI, heat treatment is often applied prior to cold storage. In the present work, metabolic profiling was performed to determine the metabolic dynamics associated with the induction of acquired CI tolerance in response to heat shock. 'Dixiland' peach fruits exposed to 39 °C, cold stored, or after a combined treatment of heat and cold, were compared with fruits ripening at 20 °C. Dramatic changes in the levels of compatible solutes such as galactinol and raffinose were observed, while amino acid precursors of the phenylpropanoid pathway were also modified due to the stress treatments, as was the polyamine putrescine. The observed responses towards temperature stress in peaches are composed of both common and specific response mechanisms to heat and cold, but also of more general adaptive responses that confer strategic advantages in adverse conditions such as biotic stresses. The identification of such key metabolites, which prime the fruit to cope with different stress situations, will likely greatly accelerate the design and the improvement of plant breeding programs.
Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI), Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Rosario, 2000, Argentina.
Martin A
MA
Lauxmann
Julia
J
Borsani
Sonia
S
Osorio
Verónica A
VA
Lombardo
Claudio O
CO
Budde
Claudia A
CA
Bustamante
Laura L
LL
Monti
Carlos S
CS
Andreo
Alisdair R
AR
Fernie
María F
MF
Drincovich
María V
MV
Lara
D003080:Cold Temperature; D005638:Fruit / Q000235:genetics / Q000378:metabolism* / Q000502:physiology*; D008401:Gas Chromatography-Mass Spectrometry; D018506:Gene Expression Regulation, Plant; D006358:Hot Temperature; D053858:Metabolic Networks and Pathways / Q000235:genetics; D055442:Metabolome / Q000235:genetics; D055432:Metabolomics; D009584:Nitrogen / Q000378:metabolism; D025341:Principal Component Analysis; D027861:Prunus / Q000235:genetics / Q000378:metabolism* / Q000502:physiology*; D019655:Quantitative Trait, Heritable; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011887:Raffinose / Q000378:metabolism
D012333:RNA, Messenger; D011887:Raffinose; D009584:Nitrogen
Prunus persica; chilling injury; stress