RNase and DNase activities of antiviral proteins from leaves of Bougainvillea xbuttiana.
Antiviral proteins (AVPs) purified from the leaves of Bougainvillea xbuttiana cv Mahara exhibited RNase activity against viral RNA of the tobamoviruses, Tobacco mosaic virus (TMV) and Sunnhemp rosette virus (SRV). They caused complete degradation of viral RNAs in a concentration-dependent manner. RNase activity gel assay ruled out the possibility of the presence of contaminating nucleases. AVPs also showed DNase activity, as indicated by conversion of supercoiled form of plasmid DNA into relaxed and linear forms. The implications of these activities in controlling plant viruses are discussed.
Division of Biochemistry, Indian Agricultural Research Institute, New Delhi 110 012, India.
Shikha
S
Bhatia
M L
ML
Lodha
D000998:Antiviral Agents / Q000302:isolation & purification / Q000378:metabolism*; D004279:DNA, Viral / Q000235:genetics; D003851:Deoxyribonucleases / Q000378:metabolism*; D039281:Nyctaginaceae / Q000254:growth & development / Q000378:metabolism* / Q000821:virology; D018515:Plant Leaves / Q000254:growth & development / Q000378:metabolism* / Q000821:virology; D010940:Plant Proteins / Q000302:isolation & purification / Q000378:metabolism*; D010942:Plant Viruses / Q000201:enzymology / Q000235:genetics / Q000472:pathogenicity; D010957:Plasmids / Q000235:genetics; D012367:RNA, Viral / Q000235:genetics; D012260:Ribonucleases / Q000378:metabolism*; D014026:Nicotiana / Q000821:virology; D014027:Tobacco Mosaic Virus / Q000201:enzymology / Q000235:genetics / Q000472:pathogenicity
D000998:Antiviral Agents; D004279:DNA, Viral; D010940:Plant Proteins; D012367:RNA, Viral; D003851:Deoxyribonucleases; D012260:Ribonucleases