Effect of the interaction between periodontitis and type 1 diabetes mellitus on alveolar bone, mandibular condyle and tibia.
OBJECTIVE
This study examined the effect of the interaction between periodontitis and type 1 diabetes mellitus on alveolar bone, mandibular condyle and tibia in animal models.


METHODS
Rats were divided into normal, periodontitis, diabetic and diabetic with periodontitis groups. After injection of streptozotocin to induce diabetes, periodontitis was induced by ligation of both lower-side first molars for 30 days. Alveolar bone loss and trabecular bone volume fraction (BVF) of the mandibular condyle and tibia were estimated via hematoxylin and eosin staining and micro-computed tomography, respectively. Osteoclastogenesis of bone marrow cells isolated from tibia and femur was assayed using tartrate-resistant acid phosphatase staining.


RESULTS
The cemento-enamel junction to the alveolar bone crest distance and ratio of periodontal ligament area in the diabetic with periodontitis group were significantly increased compared to those of the periodontitis group. Mandibular condyle BVF did not differ among groups. The BVF of tibia in the diabetic and diabetic with periodontitis groups was lower than that of the normal and periodontitis groups. Osteoclastogenesis of bone marrow cells in the diabetic groups was higher than that in the non-diabetic groups. However, the BVF of tibia and osteoclastogenesis in the diabetic with periodontitis group were not significantly different than those in the diabetic group.


CONCLUSIONS
Type 1 diabetes mellitus aggravates alveolar bone loss induced by periodontitis, but periodontitis does not alter the mandibular condyle and tibia bone loss induced by diabetes. Alveolar bone, mandibular condyle and tibia may have different responses to bone loss stimuli in the diabetic environment.
Department of Applied Life Science, The Graduate School, Yonsei University , Seoul , Republic of Korea.
Ji-Hye
JH
Kim
Dong-Eun
DE
Lee
K S Niluka Darshani
KS
Gunawardhana
Seong-Ho
SH
Choi
Gye-Hyeong
GH
Woo
Jeong-Heon
JH
Cha
Eun-Jung
EJ
Bak
Yun-Jung
YJ
Yoo
D001483:Base Sequence; D017931:DNA Primers; D003922:Diabetes Mellitus, Type 1 / Q000150:complications* / Q000473:pathology; D006801:Humans; D008335:Mandibular Condyle / Q000473:pathology*; D010518:Periodontitis / Q000150:complications* / Q000473:pathology; D060888:Real-Time Polymerase Chain Reaction; D013977:Tibia / Q000473:pathology*; D055114:X-Ray Microtomography
D017931:DNA Primers