Non-invasive ultrasonic surgery of the brain in non-human primates.
High-intensity focused ultrasound causes selective tissue necrosis efficiently and safely, namely, in the prostate, liver, and uterine fibroid. Nevertheless, ablation of brain tissue using focused ultrasound remains limited due to strong aberrations induced by the skull. To achieve ultrasonic transcranial brain ablation, such aberrations have to be compensated. In this study, non-invasive therapy was performed on monkeys using adaptive correction of the therapeutic beam and 3D simulations of transcranial wave propagation based on 3D computed tomographic (CT) scan information. The aim of the study was two-fold: induce lesions in a non-human primate brain non-invasively and investigate the potential side effects. Stereotactic targeting was performed on five Macaca fascicularis individuals. Each hemisphere was treated separately with a 15-day interval and animals were sacrificed two days after the last treatment. The ultrasonic dose delivered at the focus was increased from one treatment location to the other to estimate the thermal dose for tissue alteration. Thermal doses in the brain were determined by numerical computations. Treatment efficiency and safety were evaluated histologically. The threshold for tissue damage in the brain was measured to be between 90 and 280 cumulative equivalent minutes at 43 °C. Intravenous injection of corticoids before the treatment limited the side effects.
Institut Langevin, ESPCI-ParisTech, CNRS UMR7587, INSERM U979, Paris, France.
Fabrice
F
Marquet
Anne-Laure
AL
Boch
Mathieu
M
Pernot
Gabriel
G
Montaldo
Danielle
D
Seilhean
Mathias
M
Fink
Mickael
M
Tanter
Jean-Francois
JF
Aubry
D000305:Adrenal Cortex Hormones / Q000008:administration & dosage; D000818:Animals; D054022:Cerebrum / Q000000981:diagnostic imaging / Q000187:drug effects / Q000473:pathology / Q000601:surgery*; D003198:Computer Simulation; D004334:Drug Administration Schedule; D004867:Equipment Design; D006358:Hot Temperature; D021621:Imaging, Three-Dimensional; D007275:Injections, Intravenous; D008252:Macaca fascicularis; D023421:Models, Animal; D019635:Neurosurgical Procedures / Q000009:adverse effects / Q000295:instrumentation / Q000379:methods*; D009716:Numerical Analysis, Computer-Assisted; D011300:Preoperative Care; D011857:Radiographic Image Interpretation, Computer-Assisted; D013238:Stereotaxic Techniques; D025321:Surgery, Computer-Assisted; D013997:Time Factors; D014057:Tomography, X-Ray Computed; D014159:Transducers; D059708:Ultrasonic Surgical Procedures / Q000009:adverse effects / Q000295:instrumentation / Q000379:methods*
D000305:Adrenal Cortex Hormones