Comparative messenger RNA expression of FSHβ, LHβ, FSHR, LHR, and ERβ in high and low prolific goat breeds.
Follicle-stimulating hormone (FSH) and luteinizing hormone (LH) have a central role in follicle growth and maturation, but no clear differences between breeds with different ovulation rates have been found. Therefore, this study investigated mRNA expression of FSHβ, LHβ, FSH receptor (FSHR), LH receptor (LHR), and estrogen receptor-β (ERβ) genes in prolific Lezhi black (LB) goats and nonprolific Tibetan (TB) goats by real-time PCR. Follicles and pituitaries were recovered from goats at 12-24 h after onset of estrus. Real-time PCR analysis revealed that the expression levels of FSHβ and LHβ mRNA were significantly higher (p < 0.01) in pituitary of LB than in TB does, but the expression levels of FSHR and LHR mRNA in follicle of TB were greater (p < 0.05). Expression level of follicular ER β was not different between the two breeds. Data provide evidence that the greater ovulation rate in the LB goat as compared to the TB breed is associated with a greater gonadotropin expression during follicular phase.
a Sichuan Provincial Key-Laboratory of Protection and Utilization of Animal Genetic Resources in Qinghai-Tibet Plateau, Southwest University for Nationalities , Chengdu , P. R. China.
Xiang-Dong
XD
Zi
Lei
L
Huang
Yong
Y
Wang
Jian-Yuan
JY
Lu
D000818:Animals; D047629:Estrogen Receptor beta / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D005260:Female; D020869:Gene Expression Profiling; D006041:Goats / Q000235:genetics / Q000378:metabolism*; D006065:Gonadotropins, Pituitary / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D006080:Ovarian Follicle / Q000737:chemistry / Q000378:metabolism; D010060:Ovulation / Q000235:genetics / Q000502:physiology; D010902:Pituitary Gland / Q000737:chemistry / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D011967:Receptors, Gonadotropin / Q000032:analysis* / Q000235:genetics / Q000378:metabolism
D047629:Estrogen Receptor beta; D006065:Gonadotropins, Pituitary; D012333:RNA, Messenger; D011967:Receptors, Gonadotropin