DNA modification under mild conditions by Suzuki-Miyaura cross-coupling for the generation of functional probes.
Quick and clean: A method for Pd-catalyzed Suzuki-Miyaura cross-coupling to iododeoxyuridine (IdU) in DNA is described. Key to the reactivity is the choice of the ligand and the buffer. A covalent [Pd]-DNA intermediate was isolated and characterized. Photocrosslinking probes were generated to trap proteins that bind to epigenetic DNA modifications.
Department of Chemistry, University of Oxford, Chemistry Research Laboratory, Mansfield Road, Oxford OX1 3TA (UK).
Lukas
L
Lercher
Joanna F
JF
McGouran
Benedikt M
BM
Kessler
Christopher J
CJ
Schofield
Benjamin G
BG
Davis
D002384:Catalysis; D004247:DNA / Q000737:chemistry* / Q000235:genetics; D007065:Idoxuridine / Q000737:chemistry; D015335:Molecular Probes / Q000737:chemistry*; D009841:Oligonucleotides / Q000737:chemistry* / Q000235:genetics; D010165:Palladium / Q000737:chemistry; D019920:Photoaffinity Labels / Q000737:chemistry
D015335:Molecular Probes; D009841:Oligonucleotides; D019920:Photoaffinity Labels; D010165:Palladium; D004247:DNA; D007065:Idoxuridine
DNA; cross-coupling; oligonucleotides; palladium; photoaffinity labeling