Overexpression of GmHsp90s, a heat shock protein 90 (Hsp90) gene family cloning from soybean, decrease damage of abiotic stresses in Arabidopsis thaliana.
Hsp90 is one of the most conserved and abundant molecular chaperones and is an essential component of the protective stress response; however, its roles in abiotic stress responses in soybean (Glycine max) remain obscure. Here, 12 GmHsp90 genes from soybean were identified and found to be expressed and to function differentially under abiotic stresses. The 12 GmHsp90 genes were isolated and named GmHsp90A1-GmHsp90A6, GmHsp90B1, GmHsp90B2, GmHsp90C1.1, GmHsp90C1.2, GmHsp90C2.1 and GmHsp90C2.2 based on their characteristics and high homology to other Hsp90s according to a new nomenclature system. Quantitative real-time PCR expression data revealed that all the genes exhibited higher transcript levels in leaves and could be strongly induced under heat, osmotic and salt stress but not cold stress. Overexpression of five typical genes (GmHsp90A2, GmHsp90A4, GmHsp90B1, GmHsp90C1.1 and GmHsp90C2.1) in Arabidopsis thaliana provided useful evidences that GmHsp90 genes can decrease damage of abiotic stresses. In addition, an abnormal accumulation of proline was detected in some transgenic Arabidopsis plants suggested overexpressing GmHsp90s may affect the synthesis and response system of proline. Our work represents a systematic determination of soybean genes encoding Hsp90s, and provides useful evidence that GmHsp90 genes function differently in response to abiotic stresses and may affect the synthesis and response system of proline.
Key Laboratory of Biology and Genetics and Breeding for Soybean/National Key Laboratory of Crop Genetics and Germplasm Enhancement, Ministry of Agriculture/National Center for Soybean Improvement, Nanjing Agricultural University, Nanjing, PR China.
Jinyan
J
Xu
Chenchen
C
Xue
Dong
D
Xue
Jinming
J
Zhao
Junyi
J
Gai
Na
N
Guo
Han
H
Xing
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development; D003001:Cloning, Molecular; D018506:Gene Expression Regulation, Plant; D018841:HSP90 Heat-Shock Proteins / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D006358:Hot Temperature; D008353:Mannitol / Q000494:pharmacology; D008969:Molecular Sequence Data; D009994:Osmolar Concentration; D010802:Phylogeny; D018515:Plant Leaves / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development; D010940:Plant Proteins / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D030821:Plants, Genetically Modified; D011392:Proline / Q000378:metabolism; D020033:Protein Isoforms / Q000145:classification / Q000235:genetics / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D016415:Sequence Alignment; D012965:Sodium Chloride / Q000494:pharmacology; D013025:Glycine max / Q000737:chemistry / Q000235:genetics*; D013312:Stress, Physiological
D018841:HSP90 Heat-Shock Proteins; D010940:Plant Proteins; D020033:Protein Isoforms; D008353:Mannitol; D012965:Sodium Chloride; D011392:Proline