[Simultaneous quantitative analysis of five alkaloids in Sophora flavescens by multi-components assay by single marker].
OBJECTIVE
To establish a new method for quality evaluation and validate its feasibilities by simultaneous quantitative assay of five alkaloids in Sophora flavescens.


METHODS
The new quality evaluation method, quantitative analysis of multi-components by single marker (QAMS), was established and validated with S. flavescens. Five main alkaloids, oxymatrine, sophocarpine, matrine, oxysophocarpine and sophoridine, were selected as analytes to evaluate the quality of rhizome of S. flavescens, and the relative correction factor has good repeatibility. Their contents in 21 batches of samples, collected from different areas, were determined by both external standard method and QAMS. The method was evaluated by comparison of the quantitative results between external standard method and QAMS.


RESULTS
No significant differences were found in the quantitative results of five alkaloids in 21 batches of S. flavescens determined by external standard method and QAMS.


CONCLUSIONS
It is feasible and suitable to evaluate the quality of rhizome of S. flavescens by QAMS.
School of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou 510006, China. janechan_best@126.com
Jing
J
Chen
Shu-Mei
SM
Wang
Jiang
J
Meng
Fei
F
Sun
Sheng-Wang
SW
Liang
D000470:Alkaloids / Q000032:analysis*; D011807:Quinolizines / Q000032:analysis; D029909:Sophora / Q000737:chemistry*; D000093842:Matrines
D000470:Alkaloids; D011807:Quinolizines; C046246:oxysophocarpine; C035933:sophocarpine; C037573:oxymatrine; D000093842:Matrines