Naegleria fowleri glycoconjugates with residues of α-D-mannose are involved in adherence of trophozoites to mouse nasal mucosa.
We analyzed the possible role of glycoconjugates containing α-D-mannose and α-D-glucose residues in adherence of trophozoites to mouse nasal epithelium. Trophozoites incubated with 20 μg of one of three different lectins which preferentially recognized these residues were inoculated intranasally in Balb/c mice. Mouse survival was 40% with Pisum sativum and Canavalia ensiformis and 20% with Galanthus nivalis amebic pretreatment, compared with 0% survival for control animals administered trophozoites without pretreatment. Possibly some of the glycoproteins found in Naegleria fowleri represent an adherence factor. Differences in the saccharide sequences of the Naegleria species, even on the same glycoconjugate structure, could explain the different results corresponding to the distinct pretreatments (C. ensiformis, G. nivalis, and P. sativum). We found a higher expression of glycoconjugates recognized by P. sativum in Naegleria lovaniensis than N. fowleri, probably due to the higher number of oligosaccharides containing an α-1,6-linked fucose moiety expressed on the former species.
Sección de Estudios de Postgrado e Investigación, Escuela Superior de Medicina, Instituto Politécnico Nacional, Plan de San Luís y Díaz Mirón, México, D.F, Mexico.
Maricela
M
Carrasco-Yepez
Rafael
R
Campos-Rodriguez
Marycarmen
M
Godinez-Victoria
Marco Aurelio
MA
Rodriguez-Monroy
Adriana
A
Jarillo-Luna
Patricia
P
Bonilla-Lemus
Arturo Contis-Montes
AC
De Oca
Saul
S
Rojas-Hernandez
D000818:Animals; D002448:Cell Adhesion / Q000502:physiology*; D005434:Flow Cytometry; D005947:Glucose; D006001:Glycoconjugates / Q000737:chemistry / Q000378:metabolism*; D037102:Lectins / Q000737:chemistry / Q000378:metabolism; D008297:Male; D008358:Mannose / Q000737:chemistry*; D051379:Mice; D008807:Mice, Inbred BALB C; D009253:Naegleria / Q000502:physiology*; D009297:Nasal Mucosa / Q000378:metabolism*; D053058:Trophozoites / Q000502:physiology*
D006001:Glycoconjugates; D037102:Lectins; D005947:Glucose; D008358:Mannose