Accelerated degradation of exogenous indole by Burkholderia unamae strain CK43B exposed to pyrogallol-type polyphenols.
In modified Winogradsky's (MW) medium supplemented with excessive indole (1), Burkholderia unamae strain CK43B isolated from polyphenol-rich Shorea rhizosphere showed almost no cell growth, but it showed drastic cell growth given further supplementation of gallic acid, a simple plant polyphenol. This active cell growth of B. unamae CK43B was due to the stimulating effect of gallic acid on 1-degradation of bacterial cells, which acquired a nitrogen source in 1. Under aerobic culture conditions with appropriate concentrations (0.5-2.0 mM) of gallic acid, B. unamae CK43B started to decompose exogenous 1 in a dose-dependent manner, and finally accumulated catechol (5) via anthranilic acid (4). Pyrogallol also showed a cometabolic effect on decarboxylation-coupled oxidative deamination of B. unamae CK43B, producing 5 from 4, as gallic acid did. These results suggest that pyrogallol-type plant polyphenols act as stimulators on B. unamae CK43B, causing it to degrade an N-heterocyclic aromatic compound (NHAC) including nitrogen-containing humic substances.
Division of Applied Bioscience, Research Faculty of Agriculture, Hokkaido University.
Dongyeop
D
Kim
Atiqur
A
Rahman
Irnayuli R
IR
Sitepu
Yasuyuki
Y
Hashidoko
D019117:Burkholderia / Q000737:chemistry* / Q000254:growth & development; D053958:Dipterocarpaceae / Q000382:microbiology*; D005707:Gallic Acid / Q000737:chemistry / Q000378:metabolism; D047348:Hydrolyzable Tannins; D007211:Indoles / Q000737:chemistry / Q000378:metabolism*; D059808:Polyphenols / Q000494:pharmacology; D011748:Pyrogallol / Q000494:pharmacology; D058441:Rhizosphere; D012988:Soil Microbiology
D047348:Hydrolyzable Tannins; D007211:Indoles; D059808:Polyphenols; D011748:Pyrogallol; D005707:Gallic Acid; C030374:indole