[Alterations in the protein content and dysfunction of high-density lipoproteins from hyperhomocysteinemic mice].
OBJECTIVE
The aim of this study was to evaluate the proteic changes in high-density lipoproteins (HDL) induced by methionine-induced hyperhomocysteinemia in mice and its relationship with two of their main antiatherogenic properties.


RESULTS
The oral administration of methionine resulted in an elevation (~8 times) in the plasma concentration of homocysteine. Hyperhomocysteinemia was inversely correlated with the plasma concentration of HDL cholesterol and its main protein component of HDL, apolipoprotein (apo) A-I, respectively. The cholesterol efflux in vivo from macrophages to HDL was decreased in hyperhomocysteinemic mice compared with the control mice. However, the reverse cholesterol transport from macrophages to feces remained unchanged. On the other hand, the ability of HDL from hyperhomocysteinemic mice to prevent the oxidative modification of low-density lipoproteins (LDL) was found decreased and associated with a concomitant reduction in the plasma activity of paraoxonase-1 (PON1) and the plasma concentration of apoA-I, and with a relative reduction in the apoA-IV content (~1.5 times) in the hyperhomocysteinemic HDL, respectively.


CONCLUSIONS
The decrease in the ability of HDL from hyperhomocysteinemic mice to prevent LDL from oxidation was associated with a decrease in the apoA-I, PON1 and apoA-IV.
Institut d'Investigació Biomèdica Sant Pau (IIB-Sant Pau), Barcelona, España; CIBER de Diabetes y Enfermedades Metabólicas Asociadas, CIBERDEM, España; Departament de Bioquímica i Biologia Molecular, Universitat Autònoma de Barcelona, Barcelona, España.
Josep
J
Julve
Teresa Laura
TL
Errico
Xiangyu
X
Chen
David
D
Santos
Júlia
J
Freixa
Inmaculada
I
Porcel
Esther
E
Cubero
Joan Carles
JC
Escolà-Gil
Francisco
F
Blanco-Vaca
D000818:Animals; D016632:Apolipoprotein A-I / Q000097:blood*; D001054:Apolipoproteins A / Q000097:blood; D043303:Aryldialkylphosphatase / Q000097:blood; D001692:Biological Transport; D002784:Cholesterol / Q000378:metabolism; D008076:Cholesterol, HDL / Q000097:blood*; D005243:Feces / Q000737:chemistry; D006710:Homocysteine / Q000097:blood; D020138:Hyperhomocysteinemia / Q000097:blood / Q000378:metabolism*; D008077:Lipoproteins, LDL / Q000097:blood*; D008264:Macrophages / Q000378:metabolism; D008297:Male; D008715:Methionine / Q000494:pharmacology; D051379:Mice; D008810:Mice, Inbred C57BL; D010084:Oxidation-Reduction
D016632:Apolipoprotein A-I; D001054:Apolipoproteins A; D008076:Cholesterol, HDL; D008077:Lipoproteins, LDL; C022205:apolipoprotein A-IV; D006710:Homocysteine; D002784:Cholesterol; D008715:Methionine; D043303:Aryldialkylphosphatase
High-density lipoproteins; Hiperhomocisteinemia; Hyperhomocysteinemia; Lipoproteínas de alta densidad; Proteomics; Proteómica