Astragalin suppresses inflammatory responses via down-regulation of NF-κB signaling pathway in lipopolysaccharide-induced mastitis in a murine model.
Mastitis is a prevalent and economic disease around the world and defined as infection and inflammation of the mammary gland. Astragalin, a bioactive component isolated from persimmon or Rosa agrestis, has been reported to have anti-inflammatory properties. To investigate the potential therapeutic effect of astragalin in mastitis, a murine model of mastitis was induced by administration of LPS in mammary gland. Astragalin was applied 1h before and 12h after LPS treatment. The results showed that astragalin attenuated the infiltration of inflammatory cells, the activity of myeloperoxidase (MPO) and the expression of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and interleukin-1β (IL-1β) in a dose-dependent manner. Additionally, Western blotting results showed that astragalin efficiently blunt decreased nuclear factor-kappaB (NF-κB) activation by inhibiting the degradation and phosphorylation of IκBα and the nuclear translocation of p65. These results suggested that astragalin exerts anti-inflammatory properties in LPS-mediated mastitis, possibly through inhibiting inhibition of the NF-κB signaling pathway, which mediates the expression of pro-inflammatory cytokines. Astragalin may be a potential therapeutic agent against mastitis.
Department of Clinical Veterinary Medicine, Jilin University, Changchun, Jilin Province, People's Republic of China.
Fengyang
F
Li
Dejie
D
Liang
Zhengtao
Z
Yang
Tiancheng
T
Wang
Wei
W
Wang
Xiaojing
X
Song
Mengyao
M
Guo
Ershun
E
Zhou
Depeng
D
Li
Yongguo
Y
Cao
Naisheng
N
Zhang
D000818:Animals; D002478:Cells, Cultured; D004195:Disease Models, Animal; D015536:Down-Regulation; D005260:Female; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D044949:Kaempferols / Q000494:pharmacology*; D008070:Lipopolysaccharides / Q000276:immunology; D008413:Mastitis / Q000139:chemically induced / Q000188:drug therapy* / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D016328:NF-kappa B / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects
D007166:Immunosuppressive Agents; D044949:Kaempferols; D008070:Lipopolysaccharides; D016328:NF-kappa B; C001579:astragalin
Astragalin; Cytokine; Lipopolysaccharide (LPS); Mastitis; Nuclear factor-kappaB (NF-κB)