Occurrence of Human Cosavirus in Wastewater and River Water in Japan.
Human cosavirus was detected using reverse transcription-quantitative polymerase chain reaction in 71 % of raw sewage and 29 % each of secondary-treated sewage and river water samples in the Kofu Basin, Japan. The highest concentration was 2.80 × 10(6) copies/l. Nucleotide sequence analysis revealed multiple genotypes of the virus in wastewater.
International Research Center for River Basin Environment, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, 4-3-11 Takeda, Kofu, Yamanashi, 400-8511, Japan. eharamoto@yamanashi.ac.jp.
Eiji
E
Haramoto
International Research Center for River Basin Environment, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, 4-3-11 Takeda, Kofu, Yamanashi, 400-8511, Japan.
Mikie
M
Otagiri
D005838:Genotype; D007564:Japan; D010802:Phylogeny; D010849:Picornaviridae / Q000145:classification / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction; D045483:Rivers / Q000821:virology*; D012621:Seasons; D012722:Sewage / Q000821:virology; D062065:Wastewater / Q000821:virology*
D012722:Sewage; D062065:Wastewater
Human cosavirus; Picornavirus; Public health; River water; Wastewater