Molecular cloning and bacterial expression of the catalytic domain of the SENP1 gene of Oryzias latipes.
In this study, we cloned the catalytic domain of the Oryzias latipes sentrin/SUMO-specific protease 1 (OlSENP1-CD) gene and produced the recombinant OlSENP1-CD protein in Escherichia coli. Experimental procedures designed to reveal the ability of the recombinant protein to show deSUMOylating activity in vitro should be helpful in future studies of other SENPs and the SUMO pathway.
Department of Biological Sciences, Graduate School of Science and Technology, Kumamoto University.
Shinpei
S
Obata
Eri
E
Yuasa
Daisuke
D
Seki
Takeshi
T
Kitano
Hisato
H
Saitoh
D000818:Animals; D020134:Catalytic Domain / Q000235:genetics; D003001:Cloning, Molecular; D010450:Endopeptidases / Q000235:genetics*; D004926:Escherichia coli; D009990:Oryzias / Q000235:genetics*; D011994:Recombinant Proteins / Q000235:genetics*; D025842:SUMO-1 Protein / Q000235:genetics; D013379:Substrate Specificity
D011994:Recombinant Proteins; D025842:SUMO-1 Protein; D010450:Endopeptidases