Synthesis of leukotrienes in porcine uteri with endometritis induced by infection with Escherichia coli.
Leukotrienes (LTs) are lipid mediators that play a significant role in the inflammatory process. Their production in inflamed uteri is not fully understood. The present experiment aimed to determine LTB4 and LTC4 amounts, 5-lipooxygenase (5-LO), LTA4 hydrolase (LTAH) and LTC4 synthase (LTCS) mRNA levels and protein expression in inflamed porcine uteri. On Day 3 of the oestrous cycle (Day 0 of the study), either Escherichia coli suspension or saline were infused into uterine horns. Collection of uterine tissues and washings took place eight or sixteen days later. In gilts suffering from endometritis increased LTB4 and LTC4 levels in the endometrium and washings and 5-LO mRNA levels in the myometrium on Days 8 and 16, 5-LO protein levels in the endometrium and myometrium on Day 8, LTAH mRNA and protein levels in the endometrium and myometrium on Days 8 and 16, respectively. Although LTCS mRNA and protein expression in the myometrium and LTCS protein expression in the endometrium were enhanced on Day 16 after Escherichia coli inoculation, LTCS mRNA levels decreased on Day 8 in both tissues. Our study shows the upregulation of LT production in inflamed porcine uteri, which suggests the importance of these factors to the process of uterine inflammation.
Division of Reproductive Biology, Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences, Tuwima 10, 10-748 Olsztyn, Poland.
Barbara
B
Jana
Division of Reproductive Biology, Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences, Tuwima 10, 10-748 Olsztyn, Poland.
Joanna
J
Czarzasta
Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, University of Warmia and Mazury, Oczapowskiego 13, 10-719 Olsztyn, Poland.
Jerzy
J
Jaroszewski
D000818:Animals; D001094:Arachidonate 5-Lipoxygenase / Q000032:analysis / Q000235:genetics; D004716:Endometritis / Q000378:metabolism / Q000382:microbiology / Q000662:veterinary*; D004717:Endometrium / Q000737:chemistry; D004851:Epoxide Hydrolases / Q000032:analysis / Q000235:genetics; D004927:Escherichia coli Infections / Q000378:metabolism / Q000662:veterinary*; D005260:Female; D005982:Glutathione Transferase / Q000032:analysis / Q000235:genetics; D007975:Leukotriene B4 / Q000032:analysis; D017997:Leukotriene C4 / Q000032:analysis; D015289:Leukotrienes / Q000096:biosynthesis*; D009215:Myometrium / Q000737:chemistry; D012333:RNA, Messenger / Q000032:analysis; D034421:Sus scrofa; D013552:Swine / Q000378:metabolism* / Q000382:microbiology; D013553:Swine Diseases / Q000378:metabolism*; D007507:Therapeutic Irrigation / Q000662:veterinary; D013997:Time Factors; D015854:Up-Regulation
D015289:Leukotrienes; D012333:RNA, Messenger; D007975:Leukotriene B4; D017997:Leukotriene C4; D001094:Arachidonate 5-Lipoxygenase; D005982:Glutathione Transferase; D004851:Epoxide Hydrolases; C041116:leukotriene-C4 synthase; C043134:leukotriene A4 hydrolase