Enzymatic hydrolysis, adsorption, and recycling during hydrolysis of bagasse sulfite pulp.
The high costs of enzymatic hydrolysis along with the high enzyme dosage are often considered as the major bottlenecks in lignocellulosic bioconversion. This study investigated the hydrolysis efficiency, cellulase adsorption and enzyme recycling during the hydrolysis of bagasse sulfite pulp (BSP). After 48 h of hydrolysis, more than 70% of the cellulose was hydrolyzed, while the protein concentration and cellulase activity in solution remained 31% and 17% of the initial value, respectively. The cellulase adsorption on the fresh BSP was better fitted by a Sips model, suggesting the occurrence of a multilayer adsorption at low cellulase concentration and monolayer adsorption at high concentration on the BSP surfaces. Desorption profile studies showed that the optimum desorption condition was at pH 4.8 and 40 °C. Moreover, considering the limited ability to desorption, directly empolying the bound enzyme with residual substrate is more effective method to recover cellulase during the hydrolysis of BSP.
Jiangsu Key Lab of Biomass-Based Green Fuels and Chemicals, Nanjing 210037, People's Republic of China. Electronic address: hgouyj@njfu.edu.cn.
Jia
J
Ouyang
College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, People's Republic of China.
Baotian
B
Liu
College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, People's Republic of China.
Min
M
Zhang
College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, People's Republic of China.
Zhaojuan
Z
Zheng
College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, People's Republic of China.
Heng
H
Yu
D000327:Adsorption; D001234:Aspergillus niger / Q000378:metabolism; D056804:Biofuels; D018533:Biomass; D002480:Cellulase / Q000737:chemistry*; D002482:Cellulose / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D035901:Hypocrea / Q000378:metabolism; D008031:Lignin / Q000737:chemistry; D059027:Recycling; D013379:Substrate Specificity; D013447:Sulfites / Q000737:chemistry*; D013696:Temperature; D013997:Time Factors
D056804:Biofuels; D013447:Sulfites; C036909:lignocellulose; D002482:Cellulose; D008031:Lignin; C027433:bagasse; D002480:Cellulase
Bagasse sulfite pulp; Cellulase; Cellulase adsorption; Enzymatic hydrolysis; Enzyme recycling