Environmental surveillance of human parechoviruses in sewage in The Netherlands.
The circulation of human parechoviruses (HPeVs) in the population was studied by environmental surveillance comprising of molecular analyses of sewage samples (n = 89) that were collected from 15 different locations in The Netherlands. Samples were taken from sewage originating from schools (n = 9) or from parts of municipalities (n = 6) during the Dutch school year 2010-2011. At 13/15 locations HPeV1, HPeV3, or HPeV6 RNA was detected at least once; however, sequence diversity did not reflect associations in time or place. A higher percentage of positives was observed in the samples originating from the municipalities. It was demonstrated that HPeV circulated in the studied population to a higher extent than would be expected from the current knowledge on infections predominating in young children.
Laboratory for Zoonoses and Environmental Microbiology at the Centre for Infectious Disease Control Netherlands, National Institute of Public Health and the Environment (RIVM).
W J
WJ
Lodder
M
M
Wuite
A M
AM
de Roda Husman
S A
SA
Rutjes
D002947:Cities; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D008969:Molecular Sequence Data; D009426:Netherlands; D030061:Parechovirus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D012367:RNA, Viral / Q000235:genetics; D012574:Schools; D017422:Sequence Analysis, DNA; D012722:Sewage / Q000821:virology*
D012367:RNA, Viral; D012722:Sewage