[Expression down-regulation of c-Cbl and Cbl-b genes in peripheral blood mononuclear cells from multiple myeloma patients].
OBJECTIVE
To investigate c-Cbl and Cbl-b gene expressions in peripheral blood mononuclear cells (PBMCs) from multiple myeloma (MM) patients.


METHODS
SYBR(R); Green PCR technique was used to detect c-Cbl and Cbl-b gene expressions in PBMCs from 23 MM patients and 22 healthy individuals, and RT-PCR and DNA sequence analysis were performed to analyze the mutations of 7-10 exons of c-Cbl.


RESULTS
The expression of c-Cbl gene in MM patients (median: 0.798%) significantly decreased as compared with that in healthy controls (median: 2.443%) (P<0.05). The expression of Cbl-b gene in MM patients (median: 0.714%) also dropped significantly as compared with that in healthy controls (median: 2.179%) (P<0.05). The 7-10 exons of c-Cbl gene had two different sizes of fragments in 2 MM patients: 483 bp and 148 bp which were wild-type and deletion mutants type of c-Cbl gene. c-Cbl gene mutations were not found in all MM patients.


CONCLUSIONS
The expressions of c-Cbl and Cbl-b genes in PBMCs from MM patients are down-regulated.
Institute of Hematology, Jinan University, Guangzhou, China.
Su
S
Fang
Xiangyu
X
Guo
Xiuxiu
X
Leng
Bo
B
Li
Yuzhe
Y
Niu
Shaohua
S
Chen
Lijian
L
Yang
Yangqiu
Y
Li
D000328:Adult; D000368:Aged; D001483:Base Sequence; D015536:Down-Regulation; D005260:Female; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000378:metabolism / Q000502:physiology*; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D009101:Multiple Myeloma / Q000097:blood* / Q000235:genetics*; D050721:Proto-Oncogene Proteins c-cbl / Q000096:biosynthesis* / Q000097:blood / Q000235:genetics*; D055815:Young Adult
D050721:Proto-Oncogene Proteins c-cbl; C067419:CBL protein, human