Association between cytosolic glycerol 3-phosphate dehydrogenase gene expression in human subcutaneous adipose tissue and BMI.
OBJECTIVE
Cytosolic glycerol 3-phosphate dehydrogenase (cGPDH) is a key enzyme providing glycerol 3-phosphate for triacylglycerol synthesis in adipose tissue and is regarded as a marker for adipocyte differentiation. The aim of this study was to test the hypothesis that an increase in cGPDH gene expression in subcutaneous adipose tissue is associated with obesity.


METHODS
mRNA levels in human subcutaneous adipose tissue were analysed by Real-Time PCR.


RESULTS
We found that human subcutaneous adipose tissue cGPDH activity and cGPDH mRNA level were greater in obese patients than in lean subjects and were positively correlated with BMI and fat mass. Moreover, a strong positive correlation between subcutaneous adipose tissue cGPDH mRNA level and cGPDH activity was found. The data presented here indicates also that PPARγ mRNA level is positively correlated with body mass index and fat mass as well as with adipose tissue cGPDH mRNA level. Moreover, the association between subcutaneous adipose tissue cGPDH mRNA level and fatty acid translocase (FAT/CD36) mRNA level was also observed.


CONCLUSIONS
The obtained results suggest that in comparison to lean subjects the increase in subcutaneous adipose tissue cGPDH gene expression in the obese, is probably the result of adipose tissue expansion during obesity.
Department of Pharmaceutical Biochemistry, Medical University of Gdansk, Gdansk, Poland.
Tomasz
T
Sledzinski
Justyna
J
Korczynska
Elzbieta
E
Goyke
Tomasz
T
Stefaniak
Monika
M
Proczko-Markuszewska
Lukasz
L
Kaska
Julian
J
Swierczynski
D000328:Adult; D001786:Blood Glucose / Q000032:analysis; D015992:Body Mass Index; D003600:Cytosol / Q000201:enzymology*; D005260:Female; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D005993:Glycerolphosphate Dehydrogenase / Q000235:genetics* / Q000378:metabolism*; D005994:Glycerophosphates / Q000378:metabolism; D006801:Humans; D007328:Insulin / Q000097:blood / Q000737:chemistry; D008297:Male; D008875:Middle Aged; D009765:Obesity / Q000201:enzymology; D050151:Subcutaneous Fat / Q000201:enzymology*
D001786:Blood Glucose; D005994:Glycerophosphates; D007328:Insulin; C029620:alpha-glycerophosphoric acid; D005993:Glycerolphosphate Dehydrogenase