Changes in ADF/destrin expression in the development of hair cells following Atoh1-induced ectopic regeneration.
The aim of this study was to investigate the effects of actin depolymerizing factor (ADF)/destrin and position changes of kinetosomes in the development of hair cells following Atoh1-induced ectopic regeneration in the basilar membrane of mice. We observed through immunofluorescence at various time-points the expression of ADF/destrin and the specific kinetosome marker, γ-tubulin, in hair cells following ectopic regeneration induced by adenovirus transfection, overexpression of Atoh1 andin vitroculture. Changes of ADF/destrin distribution and kinetosome position duringin vitroculture of new hair cells [Myo7a(+)] following Atoh1-induced ectopic regeneration are consistent with the changes in ADF/destrin expression and the polar migration of kinetosomes in hair cells of the cochlear sensory epithelium in normal development. ADF/destrin is involved in the development of the auditory epithelium and the development and structural rearrangement of ectopically regenerated hair cells in mammals. The kinetosomes of hair cells following Atoh1-induced ectopic regeneration show positional changesin vitroat different time-points.
Department of Otolaryngology, Otology and Skull Base Surgery;
Kai
K
Jin
Dong-Dong
DD
Ren
Fang-Lu
FL
Chi
Juan-Mei
JM
Yang
Yi-Bo
YB
Huang
Wen
W
Li
actin depolymerizing factor/destrin; hair cell; kinocilium; planar cell polarity