[Expression of BNP in rat myocardial tissue after acute cardiac dysfunction].
OBJECTIVE
To investigate the expression of brain natriuretic peptide (BNP) in rat myocardial tissue after acute cardiac dysfunction and to explore the role of BNP in diagnosis of cardiac dysfunction in forensic practice.


METHODS
Rat models of acute cardiac dysfunction were established. The expression of BNP protein and BNP mRNA in myocardial tissue after cardiac dysfunction were detected by immunohistochemistry, Western blotting and real-time RT-PCR.


RESULTS
The extent of positive staining of BNP increased over the time course during cardiac dysfunction. The expression of BNP showed mild positive in cardiomyocytes from 1 h to 2 h. From 4 h to 6 h, the expression was moderate positive. From 10 h to 12 h, the BNP showed a strongest positive expression. The expression of BNP presented a significant raise with the increasing time of cardiac dysfunction by Western blotting and real-time RT-PCR. The expression of BNP mRNA increased significantly 1 h after cardiac dysfunction.


CONCLUSIONS
Investigating the expression of BNP protein and BNP mRNA in myocardial tissue may provide a new approach to evaluate the cardiac function for forensic pathologists.
School of Forensic Medicine, China Medical University, Shenyang 110001, China. shangyi-gaoweimin@126.com
Wei-Min
WM
Gao
Zhi-Peng
ZP
Cao
Li
L
Mi
Zhong-Bo
ZB
Du
Hitoshi
H
Maeda
Bao-Li
BL
Zhu
D000818:Animals; D004195:Disease Models, Animal; D049429:Forensic Pathology; D005786:Gene Expression Regulation; D006352:Heart Ventricles / Q000378:metabolism; D006439:Hemodynamics; D008297:Male; D009203:Myocardial Infarction / Q000378:metabolism* / Q000473:pathology; D009206:Myocardium / Q000378:metabolism* / Q000473:pathology; D020097:Natriuretic Peptide, Brain / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors
D012333:RNA, Messenger; D020097:Natriuretic Peptide, Brain