Culturing fetal precursor cells using free floating serum-free conditions.
The propagation of neural precursors in culture is an essential tool for the study of the signaling matrix that regulates their proliferation, self-renewal, and generation of terminally differentiated progeny. Neural precursors can be expanded in vitro using both adherent and non-adherent culture protocols. The culture of fetal human neural precursors in the absence of serum as free-floating clusters of cells termed neurospheres is described here.
Department of Cell Biology and Anatomy, Faculty of Medicine, Hotchkiss Brain Institute, University of Calgary, Calgary, AB, Canada.
Andrew
A
Chojnacki
Samuel
S
Weiss
D000818:Animals; D002454:Cell Differentiation; D016895:Culture Media, Serum-Free; D004815:Epidermal Growth Factor / Q000502:physiology; D005333:Fetus / Q000166:cytology; D006801:Humans; D058953:Neural Stem Cells / Q000502:physiology*; D061251:Primary Cell Culture; D018874:Spheroids, Cellular / Q000502:physiology
D016895:Culture Media, Serum-Free; D004815:Epidermal Growth Factor