Rapid determination of catecholamines in urine samples by nonaqueous microchip electrophoresis with LIF detection.
A method was developed for the rapid separation of catecholamines by nonaqueous microchip electrophoresis with LIF detection, A homemade pump-free negative pressure sampling device was used for rapid bias-free sampling in nonaqueous microchip electrophoresis, the injection time was 0.5 s and the electrophoresis separation conditions were optimized. Under the optimized conditions, the samples were separated completely in less than 1 min. The average migration times of the epinephrine, dopamine, and norepinephrine were 34.26, 43.81, and 50.07 s, with a relative standard deviation of 1.05, 1.26 and 0.89% (n = 7), respectively. The linearity of the method ranged from 0.0125 to 2.0 mg/L for epinephrine and 0.025∼4.0 mg/L for dopamine, and norepinephrine, with correlation coefficients ranging between 0.9978 and 0.9986. The detection limits of epinephrine, dopamine, and norepinephrine were 2.5, 5.0 and 5.0 μg/L, respectively. The recoveries of epinephrine, dopamine, and norepinephrine in spiked urine samples were between 86 and 103%, with relative standard deviations of 4.5∼6.8% (n = 5). The proposed nonaqueous microchip electrophoresis with laser induced fluorescence detection system combined with a pump-free negative pressure sampling device was a simple, inexpensive, energy efficient, miniaturized system that can be successfully applied for the determination of catecholamines in urine samples. This article is protected by copyright. All rights reserved.
Key Lab of Mariculture and Enhancement of Zhejiang Province, Marine Fishery Institute of Zhejiang Province, Zhoushan, 316100, China.
Hongmei
H
Hu
Zhenhua
Z
Li
Xiaoning
X
Zhang
Chunxiu
C
Xu
Yuanming
Y
Guo
Catecholamines; Method development; Microchip electrophoresis; Nonaqueous; urine samples