Anti-Toxoplasma gondii antibody detection in serum and urine samples by enzyme-linked immunosorbent assay in HIV-infected patients.
BACKGROUND
Toxoplasmosis is a common parasitic infection of man, and reactivation of latent disease in HIV-infected patients can cause fatal encephalitis. Diagnosis depends on demonstration of parasite-specific antibodies in serum. In HIV-infected patients, IgM is often undetectable, whereas IgG remains detectable in the majority. Urine sample is very easily available and has not been evaluated for immunodiagnosis of toxoplasmosis.


OBJECTIVE
The study was an effort to find whether urine sample can be used in place of serum for immunodiagnosis of toxoplasmosis.


METHODS
Enzyme-linked immunosorbent assay (ELISA) was carried out in serum and urine samples collected from 100 HIV-infected patients to detect anti-toxoplasma IgG and IgM antibodies and whether positivity correlated with the CD4 T-cell counts of patients.


RESULTS
In this study, we observed that there was no significant difference in positivity of anti-toxoplasma IgM and IgG between serum and urine samples of HIV-infected patients by ELISA. There was a negative correlation between CD4 count and seropositivity.


CONCLUSIONS
Urine sample can be satisfactorily used in place of serum for immunodiagnosis of toxoplasmosis.
Department of Microbiology, KGMU, Lucknow, Uttar Pradesh, India.
Sayan
S
Bhattacharyya
Sumeeta
S
Khurana
Mohan Lal
ML
Dubey
D000293:Adolescent; D000328:Adult; D000913:Antibodies, Protozoan / Q000097:blood / Q000652:urine*; D018791:CD4 Lymphocyte Count; D019411:Clinical Laboratory Techniques / Q000379:methods*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D015658:HIV Infections / Q000150:complications*; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood / Q000652:urine; D007075:Immunoglobulin M / Q000097:blood / Q000652:urine; D008297:Male; D008875:Middle Aged; D044967:Serum / Q000737:chemistry; D014122:Toxoplasma / Q000276:immunology*; D014123:Toxoplasmosis / Q000175:diagnosis*; D014556:Urine / Q000737:chemistry*; D055815:Young Adult
D000913:Antibodies, Protozoan; D007074:Immunoglobulin G; D007075:Immunoglobulin M