CD11b(+) cells in donor-specific transfusion prolonged allogenic skin graft survival through indoleamine 2,3-dioxygenase.
The aim of this study is to show the effect of donor-specific transfusion (DST) in inducing immunological tolerance mediated by regulatory T cells (Treg) and indoleamine 2,3-dioxygenase (IDO). Skin grafts from H2(d) Balb/c were transplanted into H2(k) C3H/He 7days after the infusion of donor splenocytes, isolated each immune cell populations. Graft survival prolonged in recipients who received splenocytes, MHC class II(+) CD90(-) cells and CD3(-)CD19(-) cells (p<0.001, p<0.05 and p<0.01, respectively). CD11b(+) cell infusion resulted in prolongation of graft survival when compared to CD11c(+) cell infusion (p<0.01). Foxp3(+)CD4(+)CD25(+) T cells were increased after the transplant in recipients infused with CD11b(+) cells (p<0.05). The mixed lymphocyte reaction showed donor-specificity (p<0.001). High IDO expression was observed in CD11b(+) cell infusion group. Graft survival with DST using IDO antagonist (1MT) were not prolonged. In conclusion, DST allows induction of donor-specific tolerance which involves Foxp3(+)CD4(+)CD25(+) T cells and IDO expression.
Baylor Institute for Immunology Research, Baylor Research Institute, 3434 Live Oak, Dallas, TX 75204, USA. tikemoto@clin.med.tokushima-u.ac.jp
Tetsuya
T
Ikemoto
Morihito
M
Takita
Marlon F
MF
Levy
Mitsuo
M
Shimada
Bashoo
B
Naziruddin
D000818:Animals; D001803:Blood Transfusion; D039481:CD11b Antigen / Q000276:immunology / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D006085:Graft Survival / Q000276:immunology*; D007108:Immune Tolerance / Q000276:immunology*; D007150:Immunohistochemistry; D050503:Indoleamine-Pyrrole 2,3,-Dioxygenase / Q000276:immunology* / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D016038:Skin Transplantation / Q000276:immunology*; D050378:T-Lymphocytes, Regulatory / Q000276:immunology; D014184:Transplantation, Homologous
D039481:CD11b Antigen; D050503:Indoleamine-Pyrrole 2,3,-Dioxygenase
5-bromo-2′-deoxyuridine; APC; BrdU; DC; DST; Donor-specific transfusion; IDO; IL; INF; Immunological tolerance; Indoleamine 2,3-doxygenase; MACS; MLR; NK; Regulatory T cell; antigen-presenting cells; dendritic cell; donor-specific transfusion; indoleamine 2,3-dioxygenase; interferon; interleukin; magnetic cell sorting; mixed lymphocyte reaction; natural killer