Effects of Cd, Zn and Cd + Zn combination on ATPase activitiy in the gill and muscle of tilapia (Oreochromis niloticus).
This study investigated the responses of Na(+)/K(+)-ATPase, Mg(2+)-ATPase and Ca(2+)-ATPase in the gill and muscle of a freshwater fish Oreochromis niloticus exposed to 1 μg/mL of Cd and Zn and their mixture for different periods (0, 7, 14, 21 and 28 days). At the end of experimental periods, the activities Na(+)/K(+)-ATPase, Mg(2+)-ATPase and Ca(2+)-ATPase in gill tissues and only Ca(2+)-ATPase activity in muscle tissues were measured. Gill Na(+)/K(+)-ATPase activity generally decreased following single metal exposures, whereas their combinations increased its activity. Gill Ca(2+)-ATPase activity decreased relative to the control at most exposure times for single exposures of Zn and Cd, as well as for the combined exposure. There was no gill Ca(2+)-ATPase activity after 28 days of exposure to Zn and Cd combined. Mg(2+)-ATPase activity was not affected significantly in gill tissue by exposure to Zn and Cd individually or in combination. Muscle Ca(2+)-ATPase activity also decreased significantly following metal exposure, but not as greatly as in the gill tissue. Tissue protein levels were mostly unaffected by metal exposures. This study showed that certain ATPases are highly sensitive to metal exposure whether the metals are essential or non essential, and suggests using gill tissue Na(+)/K(+)-ATPase and Ca(2+)-ATPase as sensitive biomarkers in metal contaminated waters.
Department of Biology, Faculty of Science and Letters, Çukurova University, Balcali, 01330, Adana, Turkey.
Ali
A
Eroglu
Mustafa
M
Canli
D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D023681:Cichlids / Q000378:metabolism*; D005880:Gills / Q000187:drug effects* / Q000378:metabolism; D008670:Metals / Q000378:metabolism / Q000633:toxicity*; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D014874:Water Pollutants, Chemical / Q000378:metabolism / Q000633:toxicity*
D015415:Biomarkers; D008670:Metals; D014874:Water Pollutants, Chemical; D000251:Adenosine Triphosphatases; D000254:Sodium-Potassium-Exchanging ATPase