Ageing and colony-forming efficiency of human hair follicle keratinocytes.
The decline of tissue regenerative potential of skin and hair is a hallmark of physiological ageing and may be associated with age-related changes in tissue-specific stem cells and/or their environment. Human hair follicles (hHF) contain keratinocytes having the property of stem cells such as clonogenic potential. Growth capacity of hHF keratinocytes shows that most of the colony-forming cells are classified as holoclones, meroclones or paraclones when analysed in a clonal assay (Cell, Volume 76, page 1063). Despite the well-known impact of ageing on human hair growth, little is known about changes in hHF keratinocyte clonogenic potential with age. This study aimed at assessing the clone-forming efficiency (CFE) of hHF keratinocytes from three age groups of human donors. It demonstrates that ageing affects hHF keratinocyte CFE.
Department of Biological and Clinical Research, L'Oreal Advanced Research, Clichy, France.
Jennifer
J
Lecardonnel
Nathalie
N
Deshayes
Gaïanne
G
Genty
Nathalie
N
Parent
Bruno A
BA
Bernard
Michelle
M
Rathman-Josserand
Maryline
M
Paris
D000293:Adolescent; D000328:Adult; D053687:Adult Stem Cells / Q000166:cytology; D000368:Aged; D000375:Aging / Q000473:pathology*; D003114:Colony-Forming Units Assay; D018859:Hair Follicle / Q000166:cytology*; D006801:Humans; D015603:Keratinocytes / Q000166:cytology*; D008875:Middle Aged; D055815:Young Adult
ageing; clone-forming efficiency; holoclones; human hair follicle keratinocytes