Effect of erythropoietin on healing of corneal epithelial defects in rabbits.
OBJECTIVE
Corneal epithelial defects may heal slowly in patients with diabetes, limbal stem cell deficiency, extensive chemical burns or anesthetized corneas. Studies have shown that erythropoietin, a glycoprotein hormone that promotes red blood cell proliferation and inhibits apoptosis of erythroid progenitors, may also exert a cytoprotective, antiapoptotic effect on nonhematopoietic cells. The aim of the study was to examine the effect of erythropoietin on the healing process of corneal epithelial erosions in rabbit eyes.


METHODS
Fifteen New Zealand albino rabbits were divided into 3 groups following induction of unilateral uniform corneal epithelial erosions. The first group received local treatment with erythropoietin-containing cellulose-based gel 4 times daily; the second group received treatment with cellulose-based gel without erythropoietin 4 times daily, and the third group received no treatment. The healing process was monitored twice daily using cobalt-blue-filtered slit lamp photography and digital images of fluorescein-stained corneas until complete re-epithelization was achieved. Following re-epithelization, corneas were removed for histologic processing. One-way analysis of variance and Mann-Whitney tests were used for statistical analysis.


RESULTS
Mean ± SD time to complete re-epithelization was 55 ± 2.19 h in the group treated with erythropoietin-containing cellulose-based gel, 66.5 ± 14.25 h in the group treated with gel only and 62.2 ± 9.09 h in the untreated group (p = 0.16, not significant). There was no significant difference among the groups in the time to complete re-epithelization or the rate of epithelial healing. Histologic corneal evaluation revealed stromal vascularization in 2 of the 6 erythropoietin-treated rabbits and in neither of the control groups.


CONCLUSIONS
Erythropoietin has no beneficial effect on the rate of healing of corneal epithelial erosions in rabbit eyes, and corneal stroma neovascularization seems to be a significant adverse effect.
Department of Ophthalmology, Rabin Medical Center, Petach Tikva, Israel. eitanlivny @ gmail.com
Eitan
E
Livny
Tami
T
Livnat
Maxim
M
Yakimov
Muhannad
M
Masoud
Dov
D
Weinberger
Irit
I
Bahar
D000818:Animals; D003316:Corneal Diseases / Q000175:diagnosis / Q000188:drug therapy* / Q000503:physiopathology; D016510:Corneal Neovascularization / Q000139:chemically induced; D003319:Corneal Stroma / Q000098:blood supply; D004195:Disease Models, Animal; D019573:Epithelium, Corneal / Q000187:drug effects* / Q000293:injuries / Q000503:physiopathology; D004921:Erythropoietin / Q000009:adverse effects / Q000494:pharmacology*; D015590:Fluorophotometry; D011817:Rabbits; D011994:Recombinant Proteins; D013997:Time Factors; D014945:Wound Healing / Q000187:drug effects*
D011994:Recombinant Proteins; D004921:Erythropoietin