Generation of bone marrow and fetal liver chimeric mice.
The creation of bone marrow and fetal liver chimeric mice has proven to be a valuable tool in the field of immunology. Chimeric mice are used to study the contribution of various cell types of hematopoietic versus non-hematopoietic origin in the course of an immune response. In this chapter, we describe a detailed method to obtain bone marrow or fetal liver chimeric mice and assess the efficiency of donor cells to repopulate the hematopoietic compartment of recipient mice.
Department of Surgery, Duke University, Durham, NC, USA.
Eda K
EK
Holl
D000818:Animals; D000906:Antibodies / Q000276:immunology; D001854:Bone Marrow Cells / Q000276:immunology; D016026:Bone Marrow Transplantation / Q000379:methods*; D002678:Chimera / Q000276:immunology*; D005333:Fetus / Q000276:immunology; D006801:Humans; D016031:Liver Transplantation / Q000379:methods*; D051379:Mice
D000906:Antibodies