Kinetic studies of the effect of a 17-nucleotide difference in the 0.3-kb region containing the R-U5-5' leader sequence of Friend murine leukemia virus on viral gene expression.
In addition to the env gene, a 0.3-kb fragment containing the R-U5-5' leader sequence is essential for the induction of spongiform neurodegeneration by Friend murine leukemia virus (Fr-MLV) clone A8 and it also influences expression of the Env protein. Kinetic studies were carried out using two recombinant viruses, R7f, carrying the A8 0.3-kb fragment, and Rec5, carrying the 0.3-kb fragment of the non-neuropathogenic Fr-MLV clone 57. These analyses suggested that the 0.3-kb fragment influenced the expression level of the Env protein by regulating the amount of spliced env-mRNA rather than the amount of total viral mRNA or viral production.
Department of Bioinformatics, Faculty of Engineering, Soka University, 1-236, Tangi-machi, Hachioji-shi, Tokyo, 192-8577, Japan.
Yeng Cheng
YC
Choo
Yohei
Y
Seki
Sayaka
S
Takase-Yoden
D020121:5' Untranslated Regions; D000818:Animals; D005622:Friend murine leukemia virus / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D015967:Gene Expression Regulation, Viral; D015686:Gene Products, env / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D007700:Kinetics; D051379:Mice; D012326:RNA Splicing; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D012192:Retroviridae Infections / Q000662:veterinary* / Q000821:virology; D012376:Rodent Diseases / Q000821:virology*
D020121:5' Untranslated Regions; D015686:Gene Products, env; D012367:RNA, Viral
R-U5-5′ leader sequence; murine leukemia virus; neuropathogenicity; splicing