title: Development of three dimensional culture and expression of integrin heterodimers in human embryonic stem cells.
abstract: Extensive effort has been made to develop a three-dimensional (3D) system for the culture of pluripotent stem cells in human and model animals, which yields lots of benefits for monitoring cell-to-cell or cell-to-environment interaction and for suggesting alternative materials for clinical cases. Initial study using animal model moved toward supporting embryonic stem cells (ESCs) self-renewal in a synthetic scaffold conjugated with suitable peptide motifs. As results, the feeder-free, 3D acellular niche consisting of vinyl sulfone (VS)-functionalized polyethylene glycol (PEG)-based hydrogel binding with extracellular matrix analogs could support ESC self-renewal, but main stemness signals were switched in the 3D environment. We employed this PEG-based hydrogel for 3D culture of human ESCs and further adjustment of hydrogel constituent made it possible to support self-renewal of three ESC lines. In this study, we examined transcriptional and translational activity of integrin heterodimers for optimizing the 3D system by using peptide motifs and subsequently elucidated that transcription and translation of integrin α 5β 1, α 6β 1 and αVβ 5 were stronger than other heterodimers in a referenced human ESC line.
authors: Department of Agricultural Biotechnology; Seoul National University; Seoul, Korea; Department of Animal Biotechnology; Kangwon National University; Chuncheon, Korea.
authors: Seung Tae
authors: ST
authors: Lee
authors: Mi
authors: M
authors: Jang
authors: Gene
authors: G
authors: Lee
authors: Jeong
authors: J
authors: Mook Lim
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques / Q000295:instrumentation* / Q000379:methods; D002460:Cell Line; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000378:metabolism*; D006801:Humans; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D016023:Integrins / Q000378:metabolism*; D051379:Mice; D011092:Polyethylene Glycols; D021122:Protein Subunits / Q000378:metabolism
chemical_list: D016023:Integrins; D021122:Protein Subunits; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D011092:Polyethylene Glycols
keywords: 3D; acellular niche; human embryonic stem cells; hydrogel; integrin; polyethylene glycol; self-renewal
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