title: Cell detachment: post-isolation challenges.
abstract: Rare cells already have become established indicators for disease diagnosis, to help track prognosis, and in developing personalized therapy. Numerous techniques have been developed to effectively and specifically detect and sort rare cells and cell isolation techniques have gained much attention among researchers in the last few decades. Recent developments in nanotechnologies and microfluidics have been used with great promise towards these goals. The research emphasis has also shifted from simple detection with microfluidic devices to comprehensive isolation, collection and subsequent analysis with integrated and automated systems. The first challenge in post-isolation analysis is cell detachment from substrates, while keeping cells viable and unperturbed. In this review, various methods used for cell detachments are discussed. For effective cell sorting, the detachment is identified as critical criteria for selecting substrates and methods.
authors: Department of Oncology, The Second Affiliated Hospital of Southeast University, Nanjing, Jiangsu 210003, PR China.
authors: Qin
authors: Q
authors: Zheng
authors: Samir M
authors: SM
authors: Iqbal
authors: Yuan
authors: Y
authors: Wan
mesh_terms: D000818:Animals; D019556:COS Cells; D002448:Cell Adhesion; D002468:Cell Physiological Phenomena; D002469:Cell Separation; D002470:Cell Survival; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D006801:Humans; D051379:Mice; D046210:Microfluidic Analytical Techniques; D011108:Polymers
chemical_list: D011108:Polymers
keywords: Aptamers; Cell collection; Cellular viability; Enzymolysis; Microfluidic devices; Rare cells; Stimuli-responsive polymers