title: Increases of ferrous iron oxidation activity and arsenic stressed cell growth by overexpression of Cyc2 in Acidithiobacillus ferrooxidans ATCC19859.
abstract: Acidithiobacillus ferrooxidans plays an important role in bioleaching in reproducing the mineral oxidant of ferric iron (Fe(3+) ) by oxidization of ferrous iron (Fe(2+) ). The high-molecular-weight c-type cytochrome Cyc2 that is located in the external membrane is postulated as the first electron carrier in the Fe(2+) oxidation respiratory pathway of A. ferrooxidans. To increase ferrous iron oxidation activity, a recombinant plasmid pTCYC2 containing cyc2 gene under the control of Ptac promoter was constructed and transferred into A. ferrooxidans ATCC19859. The transcriptional level of cyc2 gene was increased by 2.63-fold and Cyc2 protein expression was observed in the recombinant strain compared with the control. The ferrous iron oxidation activity and the arsenic stressed cell growth of the recombinant strain were also elevated.
authors: State Key Laboratory of Microbial Technology, Shandong University, Jinan, People's Republic of China; Institute of Agro-Food Science and Technology, Shandong Academy of Agricultural Sciences, Jinan, People's Republic of China.
authors: Wei
authors: W
authors: Liu
authors: Jianqun
authors: J
authors: Lin
authors: Xin
authors: X
authors: Pang
authors: Shuang
authors: S
authors: Mi
authors: Shuang
authors: S
authors: Cui
authors: Jianqiang
authors: J
authors: Lin
mesh_terms: D042763:Acidithiobacillus / Q000166:cytology* / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D001151:Arsenic / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects; D045304:Cytochromes c / Q000235:genetics*; D015870:Gene Expression; D005818:Genetic Engineering / Q000379:methods*; D007501:Iron / Q000378:metabolism*; D010084:Oxidation-Reduction / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects*; D010957:Plasmids / Q000235:genetics; D014158:Transcription, Genetic
chemical_list: D045304:Cytochromes c; D007501:Iron; D001151:Arsenic
keywords: Acidithiobacillus ferrooxidans; bioleaching; conjugation; cyc2; ferrous iron oxidation