title: Monophosphoryl lipid A induces bone marrow precursor cells to differentiate into myeloid-derived suppressor cells.
abstract: Myeloid-derived suppressor cells (MDSCs) and dendritic cells (DCs) are important in the immune response. In vitro, DCs are derived from myeloid precursors by stimulation with granulocyte macrophage colony‑stimulating factor and interleukin‑4. Previous studies demonstrated that lipopolysaccharide (LPS) in combination with interferon‑γ inhibited DC development but enhanced MDSC functions. Monophosphoryl lipid A (MPL), derived from LPS, is a unique immunomodulatory Toll‑like receptor 4 agonist. In the present study, MPL was used to disturb DC differentiation from myeloid precursors and it was observed that prolonged stimulation with MPL led to the accumulation of MDSCs in vitro and in vivo. In conclusion, it was demonstrated that stimulation by MPL from the beginning of cell differentiation disturbed the development of DCs and led to the accumulation of MDSCs.
authors: Department of Ophthalmology, 117th Hospital of PLA, Hangzhou, Zhejiang 310013, P.R. China.
authors: Jie
authors: J
authors: Chen
authors: Baomu
authors: B
authors: Sun
authors: Xiuhua
authors: X
authors: Zhao
authors: Dong
authors: D
authors: Liang
authors: Junxia
authors: J
authors: Liu
authors: Yong
authors: Y
authors: Huang
authors: Wanke
authors: W
authors: Lei
authors: Maomao
authors: M
authors: Chen
authors: Weimin
authors: W
authors: Sun
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000187:drug effects* / Q000502:physiology; D002454:Cell Differentiation / Q000187:drug effects* / Q000276:immunology; D049109:Cell Proliferation; D002478:Cells, Cultured; D016207:Cytokines / Q000378:metabolism; D003713:Dendritic Cells / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D005260:Female; D008050:Lipid A / Q000031:analogs & derivatives* / Q000494:pharmacology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D022423:Myeloid Cells / Q000187:drug effects / Q000502:physiology*; D010587:Phagocytosis; D010641:Phenotype; D013601:T-Lymphocytes / Q000502:physiology; D051197:Toll-Like Receptor 4 / Q000819:agonists / Q000378:metabolism
chemical_list: D016207:Cytokines; D008050:Lipid A; C493487:Tlr4 protein, mouse; D051197:Toll-Like Receptor 4; C048436:monophosphoryl lipid A