title: Nonthermal atmospheric argon plasma jet effects on Escherichia coli biomacromolecules.
abstract: Nonthermal atmospheric plasma jet, a promising technology based on ionized gas at low temperatures, can be applied for disinfection of contaminated surfaces. In this study, Escherichia coli cells and their macromolecules were exposed to the nonthermal atmospheric argon plasma jet for different time durations. Total protein, genomic DNA, and malondialdehyde (MDA) levels of E. coli were assessed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and silver staining; agarose gel electrophoresis; and measurement of absorbance at 534 nm, respectively. After exposure, the spectroscopic results of liquid samples indicated that the survival reduction of E. coli can reach to 100 % in an exposure time of 600 s. Moreover, inactivation zones of E. coli, DNA degradation, and MDA levels were significantly increased. Additionally, banding patterns of total protein were changed and amino acid concentrations increased following ninhydrin test. The experimental results suggest that the nonthermal plasma could serve as an effective instrument for both sterilizing E. coli and degrading macromolecules from the surface of the objects being sterilized.
authors: Department of Molecular and Cell Biology, Faculty of Basic Sciences, University of Mazandaran, Babolsar, 47416-95447, Mazandaran, Iran, ahcolagar@umz.ac.ir.
authors: Abasalt
authors: A
authors: Hosseinzadeh Colagar
authors: Hamed
authors: H
authors: Memariani
authors: Farshad
authors: F
authors: Sohbatzadeh
authors: Azadeh
authors: A
authors: Valinataj Omran
mesh_terms: D001128:Argon / Q000737:chemistry* / Q000494:pharmacology*; D001272:Atmosphere; D004269:DNA, Bacterial / Q000378:metabolism; D004926:Escherichia coli / Q000187:drug effects / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000378:metabolism; D050356:Lipid Metabolism / Q000187:drug effects; D046911:Macromolecular Substances / Q000378:metabolism*; D008315:Malondialdehyde / Q000378:metabolism; D009555:Ninhydrin / Q000378:metabolism; D058626:Plasma Gases / Q000737:chemistry*; D013696:Temperature
chemical_list: D004269:DNA, Bacterial; D029968:Escherichia coli Proteins; D046911:Macromolecular Substances; D058626:Plasma Gases; D008315:Malondialdehyde; D001128:Argon; D009555:Ninhydrin