title: Effects of antimicrobial coatings and cryogenic freezing on survival and growth of Listeria innocua on frozen ready-to-eat shrimp during thawing.
abstract: Foodborne pathogens such as Listeria monocytogenes could pose a health risk on frozen ready-to-eat (RTE) shrimp as the pathogen could grow following thawing. In this study, antimicrobial-coating treatments alone, or in combination with cryogenic freezing, were evaluated for their ability to inhibit the growth of Listeria innocua, a surrogate for L. monocytogenes, on RTE shrimp. Cooked RTE shrimp were inoculated with L. innocua at 3 population levels and treated with coating solutions consisting of chitosan, allyl isothiocyanate (AIT), or lauric arginate ester (LAE). The treated shrimp were then stored at -18 °C for 6 d before being thawed at 4, 10, or 22 °C for either 24 or 48 h. Results revealed that antimicrobial coatings achieved approximately 5.5 to 1 log CFU/g reduction of L. innocua on RTE shrimp after the treatments, depending on the inoculated population levels. The coating-treated shrimp samples had significantly (P < 0.05) less L. innocua than controls at each thawing temperature and time. Cryogenic freezing in combination with coating treatments did not achieve synergistic effects against L. innocua. Antimicrobial coatings can help to improve product safety by reducing Listeria on RTE shrimp.
authors: Jiangnan Univ., 1800 Lihu Ave., Wuxi, Jiangsu 214122, China.
authors: Mingming
authors: M
authors: Guo
authors: Tony Z
authors: TZ
authors: Jin
authors: O Joseph
authors: OJ
authors: Scullen
authors: Christopher H
authors: CH
authors: Sommers
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D015169:Colony Count, Microbial; D003257:Consumer Product Safety; D005506:Food Contamination / Q000517:prevention & control*; D005516:Food Microbiology; D018857:Food Packaging / Q000379:methods; D005519:Food Preservation / Q000379:methods; D005615:Freezing; D008087:Listeria / Q000187:drug effects* / Q000254:growth & development*; D033561:Penaeidae / Q000382:microbiology; D017747:Seafood / Q000382:microbiology*; D013696:Temperature
chemical_list: D000900:Anti-Bacterial Agents
keywords: Listeria innocua; antimicrobial coating; cryogenic freezing; ready-to-eat shrimp; thawing