title: Transgene-free genome editing in Caenorhabditis elegans using CRISPR-Cas.
abstract: CRISPR-Cas is an efficient method for genome editing in organisms from bacteria to human cells. We describe a transgene-free method for CRISPR-Cas-mediated cleavage in nematodes, enabling RNA-homology-targeted deletions that cause loss of gene function; analysis of whole-genome sequencing indicates that the nuclease activity is highly specific.
authors: Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, California 91125.
authors: Hui
authors: H
authors: Chiu
authors: Hillel T
authors: HT
authors: Schwartz
authors: Igor
authors: I
authors: Antoshechkin
authors: Paul W
authors: PW
authors: Sternberg
mesh_terms: D000818:Animals; D064113:CRISPR-Cas Systems; D017173:Caenorhabditis elegans / Q000235:genetics*; D017238:Genes, Helminth; D005818:Genetic Engineering; D049751:Genome, Helminth; D006801:Humans; D009154:Mutation; D017393:RNA Editing / Q000235:genetics*; D018510:RNA, Helminth / Q000235:genetics
chemical_list: D018510:RNA, Helminth
keywords: CRISPR; Caenorhabditis elegans; genome resequencing; reverse genetics; targeted nuclease
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