title: Attenuation of fibroblast growth factor signaling by poly-N-acetyllactosamine type glycans.
abstract: Fibroblast growth factors (FGFs) and their receptors are expressed in a variety of mammalian tissues, playing a role in development and cell proliferation. While analyzing human sperm motility, we found that sperm treated with endo-β-galactosidase (EBG), which specifically hydrolyzes poly-N-acetyllactosamine type glycans (polyLacs), enhanced motility. Mass spectrometry analysis revealed that sperm-associated polyLacs are heavily fucosylated, consistent with Lewis Y antigen. Immunohistochemistry of epididymis using an anti-Lewis Y antibody before and after EBG treatment suggested that polyLacs carrying the Lewis Y epitope are synthesized in epididymal epithelia and secreted to seminal fluid. EBG-treated sperm elevated cAMP levels and calcium influx, indicating activation of fibroblast growth factor signaling. Seminal fluid polyLacs bound to FGFs in vitro, and impaired FGF-mediated signaling in HEK293T cells.
authors: Department of Gynecology and Obstetrics, Hamamatsu University School of Medicine, Hamamatsu City, Shizuoka, Japan. Electronic address: sugihara@hama-med.ac.jp.
authors: Kazuhiro
authors: K
authors: Sugihara
authors: Toshiaki K
authors: TK
authors: Shibata
authors: Kayoko
authors: K
authors: Takata
authors: Takako
authors: T
authors: Kimura
authors: Naohiro
authors: N
authors: Kanayama
authors: Roy
authors: R
authors: Williams
authors: Shingo
authors: S
authors: Hatakeyama
authors: Tomoya O
authors: TO
authors: Akama
authors: Chu-Wei
authors: CW
authors: Kuo
authors: Kay-Hooi
authors: KH
authors: Khoo
authors: Michiko N
authors: MN
authors: Fukuda
mesh_terms: D005346:Fibroblast Growth Factors / Q000378:metabolism*; D006026:Glycoside Hydrolases / Q000494:pharmacology; D057809:HEK293 Cells; D006801:Humans; D008297:Male; D011134:Polysaccharides / Q000378:metabolism / Q000502:physiology*; D011485:Protein Binding; D015398:Signal Transduction / Q000502:physiology*; D013081:Sperm Motility / Q000187:drug effects; D013094:Spermatozoa / Q000378:metabolism
chemical_list: D011134:Polysaccharides; D005346:Fibroblast Growth Factors; C037199:poly-N-acetyllactosamine; D006026:Glycoside Hydrolases; C026093:keratan-sulfate endo-1,4-beta-galactosidase
keywords: Glycan array; Heparin; Keratan sulfate; MALDI-MS; Tyrosine phosphorylation
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