title: Development of serum copper-based biological dosimetry in whole body gamma irradiation of mice.
abstract: A new biological dosimeter based on serum copper has been developed. Serum copper in mice subjected to a 60Co source at a dose rate of 0.5 Gy min-1 was detected using the bis(cyclohexanone) oxaldihydrazone colorimetric method. The dose range was from 0.5–7 Gy. The results demonstrate that serum copper decreases with increasing dose. A linear dose response is obtained. The detection limit based on serum copper is the same as that with the lower limit of dose assessment; i.e., about 1 Gy. The decrease in serum copper continues until the 28th day after gamma radiation. The absorbed doses in mice assessed using the linear curve are close to “blind” doses of 4 and 6 Gy. Therefore, serum copper is a quick, simple, and accurate biomarker for early assessment of radiation exposure of mice in the range of 0.5–7 Gy.
authors: Department of Nuclear Science and Engineering, Nanjing University of Aeronautics and Astronautics, Nanjing 210006, PR China.
authors: Xiao-hong
authors: XH
authors: Zhang
authors: Xuan-yu
authors: XY
authors: Min
authors: Ai-lian
authors: AL
authors: Wang
authors: Zhi-chao
authors: ZC
authors: Lou
authors: Ya-nan
authors: YN
authors: Zhang
authors: Xiao-dan
authors: XD
authors: Hu
authors: Hai-qian
authors: HQ
authors: Zhang
mesh_terms: D000818:Animals; D003037:Cobalt Radioisotopes / Q000009:adverse effects; D003124:Colorimetry; D003300:Copper / Q000097:blood*; D004305:Dose-Response Relationship, Drug; D005720:Gamma Rays / Q000009:adverse effects; D008297:Male; D051379:Mice; D011874:Radiometry / Q000379:methods*; D014916:Whole-Body Irradiation / Q000009:adverse effects
chemical_list: D003037:Cobalt Radioisotopes; D003300:Copper