title: The production of class III plant peroxidases in transgenic callus cultures transformed with the rolB gene of Agrobacterium rhizogenes.
abstract: The production of plant peroxidases by plant cell cultures is of great interest because of the potential for industrial applications. We used plant cell cultures overexpressing the rolB gene to produce increased amounts of plant class III peroxidases. The rolB gene ensured the stable and permanent activation of peroxidase activity in the transformed callus cultures of different plants. In particular, the total peroxidase activity in transformed Rubia cordifolia cells was increased 23-86-fold, and the abundance of the major peroxidase gene transcripts was increased 17-125-fold (depending on the level of rolB expression) compared with non-transformed control calli. The peroxidase-activating effect of rolB was greater than that of other peroxidase inducers, such as external stresses and methyl jasmonate.
authors: Institute of Biology and Soil Science of the Far East Branch of the Russian Academy of Sciences, Vladivostok 690022, Russia; Far Eastern Federal University, Vladivostok 690950, Russia.
authors: Y N
authors: YN
authors: Shkryl
authors: G N
authors: GN
authors: Veremeichik
authors: V P
authors: VP
authors: Bulgakov
authors: T V
authors: TV
authors: Avramenko
authors: E A
authors: EA
authors: Günter
authors: Y S
authors: YS
authors: Ovodov
authors: T I
authors: TI
authors: Muzarok
authors: Y N
authors: YN
authors: Zhuravlev
mesh_terms: D060054:Agrobacterium / Q000201:enzymology* / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics*; D010544:Peroxidases / Q000096:biosynthesis* / Q000235:genetics; D010940:Plant Proteins / Q000096:biosynthesis* / Q000235:genetics; D030821:Plants, Genetically Modified / Q000201:enzymology* / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D010940:Plant Proteins; D010544:Peroxidases
keywords: Agrobacterium rhizogenes; Peroxidase activity; Peroxidase production; Plant cell culture; rolB gene