title: Genotyping of IL-8-251 T > A yields prognostic information in patients with gastric carcinoma.
abstract: This study was designed to investigate the association of the IL-8-251 T > A gene polymorphism with clinicopathological features and the prognostic role of the gene polymorphism in patients with gastric adenocarcinoma. The gene polymorphism was detected by the polymerase chain reaction-restriction fragment length polymorphism method, followed by univariate and multivariate analyses to elicit its prognostic role. The frequency of IL-8-251 A/A, A/T and T/T genotypes were 11.0% (23/210), 43.8% (92/210) and 45.2% (95/210), respectively. The IL-8-251 gene polymorphism was closely correlated with depth of invasion (p = 0.007), grade of differentiation (p = 0.002) and TNM stage (p = 0.009). A/A genotype carriers showed more frequency of serosa involvement, low grade of differentiation and advanced stage of gastric carcinoma. IL-8-251 T > A gene polymorphism have no significant correlation with other clinicopathological features. The 5-year overall survival of IL-8-251 A/A genotype and T allele carriers were 30.8% and 59.2%, respectively. There is a significant discrepancy among the different genotype carriers. Multivariate analysis with the Cox regression model revealed that the IL-8-251 A/A genotype is an independent prognostic indicator (HR = 2.285, 95% Confidence Interval = 1.06-4.93, p = 0.035). We conclude that the IL-8-251 A/A genotype may indicate a poor prognosis for gastric adenocarcinoma patients.
authors: State Key Laboratory of Oncology in South China, Sun Yat-sen University Cancer Center , GuangZhou , China.
authors: Xiuyu
authors: X
authors: Cai
authors: Weihan
authors: W
authors: Hu
authors: Bei
authors: B
authors: Zhang
authors: Ni
authors: N
authors: Dai
authors: Ruihua
authors: R
authors: Xu
authors: Huijuan
authors: H
authors: Qiu
authors: Deshen
authors: D
authors: Wang
authors: Zhiming
authors: Z
authors: Li
authors: Wenqi
authors: W
authors: Jiang
mesh_terms: D000230:Adenocarcinoma / Q000175:diagnosis / Q000235:genetics* / Q000401:mortality; D000328:Adult; D000368:Aged; D005260:Female; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D005838:Genotype; D060005:Genotyping Techniques; D006801:Humans; D016209:Interleukin-8 / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D015999:Multivariate Analysis; D020641:Polymorphism, Single Nucleotide; D011379:Prognosis; D016016:Proportional Hazards Models; D017422:Sequence Analysis, DNA; D013274:Stomach Neoplasms / Q000175:diagnosis / Q000235:genetics* / Q000401:mortality
chemical_list: C508606:CXCL8 protein, human; D016209:Interleukin-8
references: Ginekol Pol. 2011 Jul;82(7):498-502
references: 21913426
references: Nat Rev Cancer. 2001 Nov;1(2):151-7
references: 11905806
references: Med Oncol. 2007;24(2):163-8
references: 17848739
references: Mol Cell Biochem. 2011 Jan;347(1-2):89-93
references: 20953818
references: Cancer Lett. 2005 Jul 28;225(2):207-14
references: 15978325
references: Cancer Sci. 2003 Aug;94(8):735-40
references: 12901801
references: Arthritis Rheum. 2002 Sep;46(9):2402-9
references: 12355488
references: Ann Oncol. 2010 Jan;21(1):78-86
references: 19622587
references: Biomarkers. 2006 Mar-Apr;11(2):184-93
references: 16766394
references: Mol Biol Rep. 2010 Dec;37(8):3983-9
references: 20300863
references: Eur Surg Res. 2005 May-Jun;37(3):166-72
references: 16088182
references: Clin Cancer Res. 2008 Jul 1;14(13):4111-8
references: 18593988
references: J Clin Gastroenterol. 2009 May-Jun;43(5):420-8
references: 19077731
references: Cytokine. 2010 Aug;51(2):158-65
references: 20621718
references: J Gastroenterol Hepatol. 2010 Oct;25(10):1604-17
references: 20880168
references: Egypt J Immunol. 2006;13(2):61-8
references: 18689272
references: Rev Med Chil. 2009 Jan;137(1):63-70
references: 19399323
references: Am J Pathol. 1998 Jan;152(1):93-100
references: 9422527
references: Clin Cancer Res. 2008 Nov 15;14(22):7554-63
references: 19010874
references: Arq Gastroenterol. 2011 Jan-Mar;48(1):46-51
references: 21537542
references: J Natl Cancer Inst. 2008 Mar 5;100(5):359-72
references: 18314475
references: Gut. 2005 Mar;54(3):330-5
references: 15710978
references: Clin Cancer Res. 2004 Nov 1;10(21):7157-62
references: 15534087
references: CA Cancer J Clin. 2011 Mar-Apr;61(2):69-90
references: 21296855
references: Eur J Cancer Prev. 2008 Feb;17(1):28-32
references: 18090907
references: Carcinogenesis. 2005 Mar;26(3):631-6
references: 15579481
references: Pharmacogenetics. 2003 Aug;13(8):501-7
references: 12893988
references: Cytokine. 2010 Jun;50(3):328-34
references: 20363644
references: Eur J Cancer. 2001 Aug;37(12):1482-7
references: 11506954
references: Int J Mol Sci. 2011;12(3):1505-18
references: 21673904
references: Cancer Epidemiol Biomarkers Prev. 2005 Nov;14(11 Pt 1):2487-93
references: 16284368
references: Med Oncol. 2011 Mar;28(1):133-6
references: 20087693
references: Biochem Biophys Res Commun. 2001 Jun 8;284(2):443-9
references: 11394899
references: Oncogene. 2003 Jan 16;22(2):256-65
references: 12527894
references: Dig Dis Sci. 2010 Jul;55(7):1818-23
references: 19777350
references: Dig Dis Sci. 2011 Jul;56(7):2017-23
references: 21243433
references: Clin Biochem. 2004 May;37(5):363-9
references: 15087251
references: Methods Mol Biol. 2011;760:1-17
references: 21779987
references: Carcinogenesis. 2009 Feb;30(2):295-9
references: 19073878
references: Chirurgia (Bucur). 2011 Mar-Apr;106(2):177-85
references: 21696062
references: J Clin Oncol. 2005 Apr 1;23(10):2339-45
references: 15800325
references: Clin Biochem. 2004 Aug;37(8):673-8
references: 15302609
references: Eur J Immunogenet. 2004 Aug;31(4):167-73
references: 15265021
references: Hum Immunol. 2011 Nov;72(11):1128-36
references: 21871937
references: Int J Cancer. 2007 Feb 1;120(3):552-62
references: 17096351
references: J Gastroenterol Hepatol. 2007 Sep;22(9):1443-9
references: 17716348
references: Br J Cancer. 1999 Oct;81(4):647-53
references: 10574250
references: Mol Biol Rep. 2012 Mar;39(3):2831-41
references: 21681427
references: Int J Cancer. 2011 May 1;128(9):2038-49
references: 20648559
references: Ann Oncol. 2008 Oct;19(10):1734-41
references: 18550579
references: Cytokine. 2008 Jul;43(1):71-5
references: 18502145
references: Scand J Gastroenterol. 2009;44(3):339-45
references: 19031173
references: Oncology. 2004;66(3):210-7
references: 15218312
references: J Gastroenterol Hepatol. 2008 Oct;23(10):1567-73
references: 18761558
references: Hum Immunol. 2006 Jan-Feb;67(1-2):13-21
references: 16698420
references: J Clin Gastroenterol. 2009 Mar;43(3):233-9
references: 18542040
references: Clin Cancer Res. 2003 Dec 1;9(16 Pt 1):5996-6001
references: 14676125
references: Am J Gastroenterol. 2010 Mar;105(3):557-64
references: 19904240
references: J Gastroenterol Hepatol. 2007 Feb;22(2):234-9
references: 17295877
references: Clin Endocrinol (Oxf). 2005 Apr;62(4):434-43
references: 15807874