title: Antigen discovery using whole-genome phage display libraries.
abstract: In the last two decades phage display technology has been used for investigating complex biological processes and isolating molecules of practical value in several applications. Bacteriophage lambda, representing a classical cloning and expression system, has also been exploited for generating display libraries of small peptides and protein domains. More recently, large cDNA and whole-genome lambda-display libraries of human pathogens have been generated for the discovery of new antigens for biomedical applications. Here, we describe the construction of a whole-genome library of a common pathogen-Streptococcus pneumoniae-and the use of this library for the molecular dissection of the human B-cell response against bacterial infection and colonization.
authors: Sigma-Tau Industrie Farmaceutiche Riunite SpA, Rome, Italy.
authors: Elisa
authors: E
authors: Beghetto
authors: Nicola
authors: N
authors: Gargano
mesh_terms: D000941:Antigens / Q000235:genetics* / Q000276:immunology; D018076:DNA, Complementary / Q000235:genetics; D006801:Humans; D019151:Peptide Library; D013296:Streptococcus pneumoniae / Q000235:genetics / Q000276:immunology
chemical_list: D000941:Antigens; D018076:DNA, Complementary; D019151:Peptide Library