title: Complete genome sequence of a Pantón-Valentine leukocidin-negative community-associated methicillin-resistant Staphylococcus aureus strain of sequence type 72 from Korea.
abstract: In the past decade, community-associated (CA-) infections with methicillin-resistant Staphylococcus aureus (MRSA) have emerged throughout the world. Different CA-MRSA strains dominate in different geographical locations. Many CA-MRSA lineages contain genes coding for the Pantón-Valentine leukocidin. However, the role of this leukotoxin in CA-MRSA pathogenesis is still controversial. The genome sequences of two key PVL-positive CA-MRSA strains (USA300, USA400) have been reported, but we lack information on the more recently found PVL-negative CA-MRSA strains. One such strain is the PVL-negative ST72, the main cause of CA-MRSA infections in Korea. Here, we report the entire genome sequence of CA-MRSA ST72 and analyze its gene content with a focus on virulence factors. Our results show that this strain does not have considerable differences in virulence factor content compared to other CA-MRSA strains (USA300, USA400), indicating that other toxins do not substitute for the lack of PVL in ST72. This finding is in accordance with the notion that differential expression of widespread virulence determinants, rather than the acquisition of additional virulence factors on mobile genetic elements, such as PVL, is responsible for the increased virulence of CA- compared to hospital-associated MRSA.
authors: Pathogen Molecular Genetics Section, Laboratory of Human Bacterial Pathogenesis, National Institute of Allergy and Infectious Diseases, US National Institutes of Health, Bethesda, Maryland, United States of America.
authors: Yan
authors: Y
authors: Chen
authors: Som S
authors: SS
authors: Chatterjee
authors: Stephen F
authors: SF
authors: Porcella
authors: Yun-Song
authors: YS
authors: Yu
authors: Michael
authors: M
authors: Otto
mesh_terms: D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry; D001427:Bacterial Toxins / Q000235:genetics*; D001483:Base Sequence; D017714:Community-Acquired Infections / Q000382:microbiology; D018899:CpG Islands / Q000235:genetics; D005098:Exotoxins / Q000235:genetics*; D005798:Genes, Bacterial; D016680:Genome, Bacterial / Q000235:genetics*; D006801:Humans; D007956:Leukocidins / Q000235:genetics*; D055624:Methicillin-Resistant Staphylococcus aureus / Q000235:genetics* / Q000821:virology; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics; D039002:Prophages / Q000235:genetics; D056910:Republic of Korea; D017422:Sequence Analysis, DNA; D037521:Virulence Factors / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D001427:Bacterial Toxins; D005098:Exotoxins; D007956:Leukocidins; C078284:Panton-Valentine leukocidin; D037521:Virulence Factors
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