title: Development and validation of RP-HPLC method for sildenafil citrate in rat plasma-application to pharmacokinetic studies.
abstract: Sildenafil citrate (SIL) is used in the treatment of erectile dysfunction and other chronic disorders. For the pharmacokinetic investigation of SIL we developed a simple and sensitive method for the estimation of SIL in rat plasma by reverse phase high-performance liquid chromatography (RP-HPLC). The drug samples were extracted by liquid-liquid extraction with 300 μl of acetonitrile and 5 ml of diethyl ether. Chromatographic separation was achieved on C18 column using methanol:water (85:15 v/v) as mobile phase at a flow rate of 1 ml/min and UV detection at 230 nm. The retention time of SIL was found to be 4.0 min having a separation time less than 5 min. The developed method was validated for accuracy, precision, linearity and recovery. Linearity studies were found to be acceptable over the range of 0.1-6 μg/ml. The method was successfully applied for the analysis of rat plasma sample for the application in pharmacokinetic study, drug interaction, bioavailability and bioequivalence.
authors: Department of Pharmacology, P. Wadhwani College of Pharmacy, Yavatmal, Maharashtra 445001, India.
authors: A S
authors: AS
authors: Tripathi
authors: I
authors: I
authors: Sheikh
authors: A P
authors: AP
authors: Dewani
authors: P G
authors: PG
authors: Shelke
authors: R L
authors: RL
authors: Bakal
authors: A V
authors: AV
authors: Chandewar
authors: P M
authors: PM
authors: Mazumder
keywords: % RSD, % relative standard deviation; DAD, diode-array detection; RP-HPLC; Rat plasma; SIL, sildenafil citrate; Sildenafil citrate
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