title: Toll-like receptor 4 is involved in myocardial damage following paraquat poisoning in mice.
abstract: The ingestion of the herbicide paraquat (PQ) can cause multiple organ injury including cardiac lesions. However, the underlying mechanism of myocardial damage is not known. Toll-like receptor 4 (TRL4) is a pattern-recognition receptor in the innate immune response to microbial pathogens. TLR4 is involved in heart dysfunction such as septic shock or myocardial ischemia. We investigated whether TLR4 would be linked to the pathogenesis of heart disease due to PQ exposure. Wild type mice (WT) and TLR4-deficient mice were injected intraperitoneally with 75mg/kg of PQ to induce myocardial damage and tested for echocardiographic assessment, histopathology, pro-inflammatory cytokine and TLR4 expression. WT mice after PQ exposure displayed deteriorate cardiac function, pathological damages, increased TLR4 mRNA and protein levels as well as myocardial TNF-α and IL-1β levels. Compared with WT mice, TLR4-deficient mice were significantly resistant to the PQ-induced injury. We concluded that the TLR4 was required as a mediator and played an important role in myocardial damage due to PQ.
authors: Department of Emergency, The First Affiliated Hospital, China Medical University, Shenyang, 110001, China.
authors: Xue-Song
authors: XS
authors: Dong
authors: Xiao-Yang
authors: XY
authors: Xu
authors: Yu-Qiang
authors: YQ
authors: Sun
authors: Wei-Liu
authors: Zhe-Hui
authors: ZH
authors: Jiang
authors: Zhi
authors: Z
authors: Liu
mesh_terms: D000818:Animals; D004562:Electrocardiography / Q000187:drug effects; D006321:Heart / Q000187:drug effects*; D006540:Herbicides / Q000633:toxicity*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009206:Myocardium / Q000473:pathology / Q000648:ultrastructure; D010269:Paraquat / Q000633:toxicity*; D017382:Reactive Oxygen Species / Q000378:metabolism; D015398:Signal Transduction; D051197:Toll-Like Receptor 4 / Q000235:genetics / Q000502:physiology*
chemical_list: D006540:Herbicides; D017382:Reactive Oxygen Species; C493487:Tlr4 protein, mouse; D051197:Toll-Like Receptor 4; D010269:Paraquat
keywords: Cardiomyocytes; Interleukin-1β; Paraquat; Pathology; Toll-like receptor 4; Toxicity; Tumor necrosis factor-α