title: The plasma mitochondrial DNA is an independent predictor for post-traumatic systemic inflammatory response syndrome.
abstract: OBJECTIVE
  Mitochondrial DNA (mtDNA), a newly identified damage-associated molecular pattern, has been observed in trauma patients, however, little is known concerning the relationship between plasma mtDNA levels and concrete post-traumatic complications, particularly systemic inflammatory response syndrome (SIRS). The aim of this study is to determine whether plasma mtDNA levels are associated with injury severity and cloud predict post-traumatic SIRS in patients with acute traumatic injury.
  
  
  METHODS
  Eighty-six consecutive patients with acute traumatic injury were prospectively enrolled in this study. The plasma mtDNA concentration was measured by a real-time, quantitative PCR assay for the human ND2 gene. The study population's clinical and laboratory data were analyzed.
  
  
  RESULTS
  The median plasma mtDNA was higher in trauma patients than in healthy controls (865.196 (251.042-2565.40)pg/ml vs 64.2147 (43.9049-80.6371)pg/ml, P<0.001) and was independently correlated with the ISS score (r=0.287, P<0.001). The plasma mtDNA concentration was also significantly higher in patients who developed post-traumatic SIRS than in patients who did not (1774.03 (564.870-10901.3)pg/ml vs 500.496 (145.415-1285.60)pg/ml, P<0.001). Multiple logistic regression analysis revealed that the plasma mtDNA was an independent predictors for post-traumatic SIRS (OR, 1.183 (95%CI, 1.015-1.379), P=0.032). Further ROC analysis demonstrated that a high plasma mtDNA level predicted post-traumatic SIRS with a sensitivity of 67% and a specificity of 76%, with a cut-off value of 1.3185 µg/ml being established, and the area under the ROC curves (AUC) was 0.725 (95% CI 0.613-0.837).
  
  
  CONCLUSIONS
  Plasma mtDNA was an independent indictor with moderate discriminative power to predict the risk of post-traumatic SIRS.
authors: Department of Respiratory Medicine, Jinling Hospital, Nanjing University School of Medicine, Nanjing, China.
authors: Xiaoling
authors: X
authors: Gu
authors: Yanwen
authors: Y
authors: Yao
authors: Guannan
authors: G
authors: Wu
authors: Tangfeng
authors: T
authors: Lv
authors: Liang
authors: L
authors: Luo
authors: Yong
authors: Y
authors: Song
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002097:C-Reactive Protein / Q000378:metabolism; D016022:Case-Control Studies; D004272:DNA, Mitochondrial / Q000097:blood*; D005260:Female; D006801:Humans; D007958:Leukocyte Count; D016015:Logistic Models; D008297:Male; D008875:Middle Aged; D011237:Predictive Value of Tests; D012372:ROC Curve; D012720:Severity of Illness Index; D018746:Systemic Inflammatory Response Syndrome / Q000097:blood* / Q000209:etiology*; D014947:Wounds and Injuries / Q000097:blood* / Q000150:complications*; D055815:Young Adult
chemical_list: D004272:DNA, Mitochondrial; D002097:C-Reactive Protein
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