title: SpoIIIE protein achieves directional DNA translocation through allosteric regulation of ATPase activity by an accessory domain.
abstract: Bacterial chromosome segregation utilizes highly conserved directional translocases of the SpoIIIE/FtsK family. These proteins employ an accessory DNA-binding domain (γ) to dictate directionality of DNA transport. It remains unclear how the interaction of γ with specific recognition sequences coordinates directional DNA translocation. We demonstrate that the γ domain of SpoIIIE inhibits ATPase activity of the motor domain in the absence of DNA but stimulates ATPase activity through sequence-specific DNA recognition. Furthermore, we observe that communication between γ subunits is necessary for both regulatory roles. Consistent with these findings, the γ domain is necessary for robust DNA transport along the length of the chromosome in vivo. Together, our data reveal that directional activation involves allosteric regulation of ATP turnover through coordinated action of γ domains. Thus, we propose a coordinated stimulation model in which γ-γ communication is required to translate DNA sequence information from each γ to its respective motor domain.
authors: From the Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts 02138.
authors: Marina
authors: M
authors: Besprozvannaya
authors: Valerie L
authors: VL
authors: Pivorunas
authors: Zachary
authors: Z
authors: Feldman
authors: Briana M
authors: BM
authors: Burton
mesh_terms: D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000494:Allosteric Regulation; D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D001412:Bacillus subtilis / Q000201:enzymology*; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D001692:Biological Transport; D004269:DNA, Bacterial / Q000378:metabolism*; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D021122:Protein Subunits / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D013329:Structure-Activity Relationship
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D021122:Protein Subunits; C030069:spore-specific proteins, Bacillus; D000251:Adenosine Triphosphatases
keywords: ATPases; Allosteric Regulation; Chromosomes; DNA-Protein Interaction; Molecular Motors
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