title: The protective effect of grape seed procyanidin extract against cadmium-induced renal oxidative damage in mice.
abstract: As an important environmental pollutant, cadmium (Cd) can lead to serious renal damage. Grape seed procyanidins extract (GSPE), a biological active component of grape seed, has been shown to possess antioxidative effects. Here, we assessed the protective effect of GSPE on Cd-induced renal damage using animal experiment. After 30 days, the oxidative damage of kidney was evaluated through measurement of superoxide dismutase (SOD), glutathione peroxidation (GSH-Px) and malondialdehyde (MDA). Since, oxidative stress could lead to apoptosis, the renal apoptosis was measured using flow cytometer. Moreover, the expression of apoptosis-related protein Bax and Bcl-2 was analyzed by immunohistochemistry and Western blot. The results showed that Cd led to the decrease of SOD and GSH-Px activities, and the increase of MDA level, induced renal apoptosis. However, the coadministration of GSPE attenuated Cd-induced lipid peroxidation, and antagonized renal apoptosis, probably associated with the expression of Bax and Bcl-2. These data suggested that GSPE has protective effect against renal oxidative damage induced by Cd, which provide a potential natural chemopreventive agent against Cd-poisoning.
authors: Department of Reproductive Medicine, First Affiliated Hospital of the Medical College, Xi'an Jiaotong University, Xi'an, Shanxi, PR China; Department of Biochemistry and Molecular Biology, Medical College of Xi'an Jiaotong University, Yanta West Road 76, Xi'an, 710061 Shanxi, PR China; Department of Toxicology, School of Public Health, Hebei Medical University, Zhongshan East Road 361, Shijiazhuang, 050017 Hebei, PR China.
authors: Qing
authors: Q
authors: Chen
authors: Rong
authors: R
authors: Zhang
authors: Wei-min
authors: WM
authors: Li
authors: Yu-jie
authors: YJ
authors: Niu
authors: Hui-cai
authors: HC
authors: Guo
authors: Xue-hui
authors: XH
authors: Liu
authors: Yu-chun
authors: YC
authors: Hou
authors: Li-juan
authors: LJ
authors: Zhao
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D044946:Biflavonoids / Q000737:chemistry / Q000494:pharmacology*; D015153:Blotting, Western; D001835:Body Weight / Q000187:drug effects; D002105:Cadmium Poisoning / Q000473:pathology / Q000517:prevention & control*; D002392:Catechin / Q000737:chemistry / Q000494:pharmacology*; D005434:Flow Cytometry; D005979:Glutathione Peroxidase / Q000378:metabolism; D007150:Immunohistochemistry; D007668:Kidney / Q000473:pathology*; D007674:Kidney Diseases / Q000139:chemically induced / Q000473:pathology* / Q000517:prevention & control; D008315:Malondialdehyde / Q000378:metabolism; D051379:Mice; D009929:Organ Size / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects; D010936:Plant Extracts / Q000494:pharmacology*; D044945:Proanthocyanidins / Q000737:chemistry / Q000494:pharmacology*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000096:biosynthesis; D012639:Seeds / Q000737:chemistry; D013482:Superoxide Dismutase / Q000378:metabolism; D027843:Vitis / Q000737:chemistry*; D051028:bcl-2-Associated X Protein / Q000096:biosynthesis
chemical_list: D000975:Antioxidants; D044946:Biflavonoids; D010936:Plant Extracts; D044945:Proanthocyanidins; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein; C017674:procyanidin; D008315:Malondialdehyde; D002392:Catechin; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase
keywords: Cadmium; Grape seed procyanidin extract; Oxidative stress; Renal damage