title: Impacts of mariculture on the diversity of bacterial communities within intertidal sediments in the Northeast of China.
abstract: Mariculture is one of the major seafood supplies worldwide and has caused serious environmental concerns on the coastal zone. Its rapid development has been shown to disrupt the sediment ecosystems and thus influence the benthic bacterial communities. Bacterial diversity and community structure within both adjacent farms and non-cultured zones intertidal sediments along the coasts of Qinhuangdao and Dalian, China, were investigated using full-length 16S rRNA gene-based T-RFLP analyses and clone library construction. Richness and Shannon-Wiener index were significantly increased at sites adjacent the mariculture farm with mean values of 29 and 2.97 from peak profiles of T-RFLP result. Clustering analyses suggested that impacts of mariculture on bacterial diversity of sediment were significantly larger than those resulted from temporal and spatial scales. Upon comparisons of RFLP patterns from 602 clones from libraries of the selected five samples, 137 OTUs were retrieved. Members of γ- and δ-Proteobacteria, Bacilli, Flavobacteria, and Actinobacteria were recorded in all libraries. In addition, γ-Proteobacteria were dominant in all samples (21.7~45.0 %). Redundancy analysis revealed that the distribution of bacterial composition seemed to be determined by the variables of salinity, PO4 (3-)-P, NH4 (+)-N, and Chlorophyll a content. The phyla of γ-Proteobacteria, Clostridia, Flavobacteria, Bacilli, and Planctomycetes were principal components to contribute to the bacterial differences of clone libraries. Our finding demonstrated that these phyla could display variations of bacterial composition linked to environmental disturbance resulted from mariculture.
authors: Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai, 264003, China.
authors: Jialin
authors: J
authors: Li
authors: Fuchao
authors: F
authors: Li
authors: Shuxian
authors: S
authors: Yu
authors: Song
authors: S
authors: Qin
authors: Guangyi
authors: G
authors: Wang
mesh_terms: D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D044822:Biodiversity; D002681:China; D017753:Ecosystem; D005398:Fisheries; D019015:Geologic Sediments / Q000032:analysis / Q000382:microbiology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012822:Silicon Dioxide / Q000032:analysis
chemical_list: D012822:Silicon Dioxide
references: FEMS Microbiol Ecol. 2008 May;64(2):175-86
references: 18336555
references: ISME J. 2010 Aug;4(8):1060-70
references: 20237512
references: Mol Biol Evol. 2005 Mar;22(3):520-32
references: 15525700
references: Appl Environ Microbiol. 2007 Aug;73(16):5261-7
references: 17586664
references: Philos Trans R Soc Lond B Biol Sci. 2010 Sep 27;365(1554):2897-912
references: 20713392
references: BMC Bioinformatics. 2009 Jun 06;10:171
references: 19500385
references: Mar Environ Res. 2010 Jul;70(1):56-64
references: 20359741
references: Mar Pollut Bull. 2009 Aug;58(3):384-95
references: 19062049
references: Microb Ecol. 2009 Oct;58(3):558-68
references: 19629578
references: Environ Sci Pollut Res Int. 2012 Jan;19(1):161-8
references: 21701862
references: Microb Ecol. 2005 Apr;49(3):451-60
references: 16003468
references: Mar Pollut Bull. 2008;57(1-5):68-77
references: 18036622
references: Environ Pollut. 2009 Jun;157(6):1745-52
references: 19286291
references: Appl Environ Microbiol. 2010 Jul;76(14):4691-702
references: 20511433
references: Microb Ecol. 2008 Oct;56(3):420-6
references: 18204798
references: FEMS Microbiol Ecol. 2005 Jun 1;53(1):141-55
references: 16329936
references: ISME J. 2010 Apr;4(4):472-87
references: 20054356
references: Environ Microbiol. 2007 May;9(5):1253-66
references: 17472638
references: Appl Microbiol Biotechnol. 2002 Mar;58(4):547-53
references: 11954805
references: FEMS Microbiol Ecol. 2009 Nov;70(2):80-92
references: 19780829
references: Mar Pollut Bull. 2008;57(6-12):707-15
references: 18439626
references: FEMS Microbiol Ecol. 2001 Jan;34(3):243-253
references: 11137604
references: J Appl Microbiol. 2012 Jan;112(1):79-89
references: 22044641
references: Microb Ecol. 2005 Oct;50(3):447-62
references: 16328662
references: Appl Microbiol Biotechnol. 2008 Sep;80(3):365-80
references: 18648804
references: ISME J. 2010 Feb;4(2):159-70
references: 19829317
references: Appl Environ Microbiol. 1999 Jan;65(1):213-20
references: 9872782
references: Syst Appl Microbiol. 2008 Aug;31(3):231-40
references: 18572341
references: Environ Int. 2009 Apr;35(3):632-46
references: 19095305
references: Chemosphere. 2011 Oct;85(6):899-919
references: 21821276
references: Microbiology (Reading). 2008 Jul;154(Pt 7):2084-2095
references: 18599836
references: FEMS Microbiol Ecol. 2003 Dec 1;46(3):233-46
references: 19719555
references: Can J Microbiol. 2011 Jun;57(6):493-503
references: 21635217
references: Proc Natl Acad Sci U S A. 2007 Dec 18;104(51):20404-9
references: 18077371
references: FEMS Microbiol Ecol. 2006 Jan;55(1):48-56
references: 16420614
references: Microb Ecol. 2005 Jan;49(1):163-75
references: 15688258
references: ISME J. 2009 Jul;3(7):780-91
references: 19340087
references: J Clin Microbiol. 1996 Mar;34(3):537-42
references: 8904410
references: Appl Environ Microbiol. 2011 Jul;77(14):4811-21
references: 21602381
references: Environ Health Perspect. 2001 Oct;109 Suppl 5:699-706
references: 11677178
references: Environ Microbiol. 2011 Oct;13(10):2752-67
references: 21883795
references: Microb Ecol. 2010 Aug;60(2):394-405
references: 20652237
references: Appl Environ Microbiol. 2000 Jul;66(7):2943-50
references: 10877790
references: Ecology. 2007 Sep;88(9):2154-61
references: 17918394