title: HI-6 modulates immunization efficacy in a BALB/c mouse model.
abstract: HI-6 is used as an antidote to nerve agents. It can also act as an antagonist to acetylcholine receptors (AChRs) including the nicotinic receptor, α 7 nAChR which is involved in regulating the immune response through macrophages. This experiment investigated the efficacy of HI-6 to regulate the immune response. Laboratory BALB/c mice received HI-6 and/or keyhole limpet hemocyanin (KLH) as an antigen. Antibody production was investigated after either 21 or 65 days when either single or repeated dose of antigen was applied. We confirmed that HI-6 significantly improved vaccination efficacy when KLH was given in a dose of 1mg/kg. The effect was dose dependent. A combination of HI-6 and KLH produced a vaccination of almost the same efficacy as that for Freund's complete adjuvant. The findings point at the suitability of HI-6 for improving vaccination efficacy at the level of immunity regulation by the nervous system.
authors: Faculty of Military Health Sciences, University of Defense, Trebesska 1575, 50001 Hradec Kralove, Czech Republic; Karel Englis College in Brno, Sujanovo namesti 356/1, 60200 Brno, Czech Republic. Electronic address: miroslav.pohanka@gmail.com.
authors: Miroslav
authors: M
authors: Pohanka
mesh_terms: D000276:Adjuvants, Immunologic / Q000494:pharmacology; D000818:Animals; D000906:Antibodies / Q000032:analysis; D000917:Antibody Formation / Q000187:drug effects; D000931:Antidotes / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005620:Freund's Adjuvant; D006433:Hemocyanins / Q000276:immunology; D007109:Immunity / Q000187:drug effects*; D007114:Immunization; D015850:Interleukin-6 / Q000032:analysis / Q000096:biosynthesis; D051379:Mice; D008807:Mice, Inbred BALB C; D010091:Oximes / Q000494:pharmacology*; D011726:Pyridinium Compounds / Q000494:pharmacology*; D058408:Th1-Th2 Balance / Q000187:drug effects
chemical_list: D000276:Adjuvants, Immunologic; D000906:Antibodies; D000931:Antidotes; D015850:Interleukin-6; D010091:Oximes; D011726:Pyridinium Compounds; D005620:Freund's Adjuvant; D006433:Hemocyanins; C032808:keyhole-limpet hemocyanin; C022870:asoxime chloride
keywords: Acetylcholine receptor; Acetylcholinesterase; Antibody; Cholinergic anti-inflammatory pathway; Inflammation; Vaccine