title: Expression of CD4+ T cell cytokine genes in the colorectal mucosa of inflammatory colorectal polyps in miniature dachshunds.
abstract: Inflammatory colorectal polyps (ICRPs) in miniature dachshunds are recently recognized as a major cause of large bowel diarrhea in this dog breed in Japan. ICRPs are characterized by the formation of multiple small polyps and a space-occupying large polyp in the colorectal area, and are thought to be a novel form of inflammatory bowel disease (IBD). In humans, specific cytokine patterns attributed to T helper (Th)1, Th17 and regulatory T cells have important roles in the pathogenesis of IBD. Thus, the aim of the present study was to assess the gene expression of cytokines of T cell subsets in the colorectal mucosa from dogs with ICRPs. Colorectal mucosal specimens from 10 dogs with ICRPs and 14 control dogs were used in this study. Interferon (IFN)-γ, interleukin (IL)-4, IL-17A and IL-10 mRNA expression was assessed using quantitative real-time PCR. IL-17A mRNA expression was significantly increased in large polyps compared to small polyps and controls. IFN-γ and IL-10 mRNA expression in large polyps were significantly higher than in controls. There was no significant difference in IL-4 mRNA expression among the three groups. IL-17A is thought to play important roles in the pathogenesis of ICRPs. IL-10 up-regulation could oppose the proinflammatory function of IL-17A.
authors: Laboratory of Veterinary Internal Medicine, Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan. Electronic address: h-ohta@vetmed.hokudai.ac.jp.
authors: Hiroshi
authors: H
authors: Ohta
authors: Kanae
authors: K
authors: Takada
authors: Shidow
authors: S
authors: Torisu
authors: Masashi
authors: M
authors: Yuki
authors: Yu
authors: Y
authors: Tamura
authors: Nozomu
authors: N
authors: Yokoyama
authors: Tatsuyuki
authors: T
authors: Osuga
authors: Sue Yee
authors: SY
authors: Lim
authors: Masahiro
authors: M
authors: Murakami
authors: Noboru
authors: N
authors: Sasaki
authors: Kensuke
authors: K
authors: Nakamura
authors: Masahiro
authors: M
authors: Yamasaki
authors: Mitsuyoshi
authors: M
authors: Takiguchi
mesh_terms: D000818:Animals; D001706:Biopsy / Q000662:veterinary; D015179:Colorectal Neoplasms / Q000235:genetics / Q000276:immunology / Q000473:pathology / Q000662:veterinary*; D016207:Cytokines / Q000235:genetics / Q000276:immunology*; D004283:Dog Diseases / Q000276:immunology* / Q000473:pathology; D004285:Dogs; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods / Q000662:veterinary; D015212:Inflammatory Bowel Diseases / Q000235:genetics / Q000276:immunology / Q000473:pathology / Q000662:veterinary*; D007413:Intestinal Mucosa / Q000276:immunology / Q000473:pathology; D007564:Japan; D008297:Male; D011127:Polyps / Q000235:genetics / Q000276:immunology / Q000473:pathology / Q000662:veterinary*; D012333:RNA, Messenger / Q000737:chemistry / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary; D018709:Statistics, Nonparametric; D016176:T-Lymphocyte Subsets / Q000276:immunology*
chemical_list: D016207:Cytokines; D012333:RNA, Messenger
keywords: Cytokine; Inflammatory colorectal polyps; Miniature dachshund; T helper cell