title: Osteogenetic properties of electrospun nanofibrous PCL scaffolds equipped with chitosan-based nanoreservoirs of growth factors.
abstract: Bioactive implants intended for rapid, robust, and durable bone tissue regeneration are presented. The implants are based on nanofibrous 3D-scaffolds of bioresorbable poly-ϵ-caprolactone mimicking the fibrillar architecture of bone matrix. Layer-by-layer nanoimmobilization of the growth factor BMP-2 in association with chitosan (CHI) or poly-L-lysine over the nanofibers is described. The osteogenetic potential of the scaffolds coated with layers of CHI and BMP-2 is demonstrated in vitro, and in vivo in mouse calvaria, through enhanced osteopontin gene expression and calcium phosphate biomineralization. The therapeutic strategy described here contributes to the field of regenerative medicine, as it proposes a route toward efficient repair of bone defects at reduced risk and cost level.
authors: INSERM UMR 1109, Osteoarticular and Dental Regenerative Nanomedicine, 11 rue Humann, 67085, Strasbourg Cedex, France; Institut de Chimie et Procédés pour l'Énergie, l'Environnement et la Santé, ICPEES-UMR 7515, Université de Strasbourg, CNRS, Institut Carnot MICA, École Européenne de Chimie, Polymères et Matériaux, 25 rue Becquerel, 67087, Strasbourg, cedex 2, France.
authors: Alice
authors: A
authors: Ferrand
authors: Sandy
authors: S
authors: Eap
authors: Ludovic
authors: L
authors: Richert
authors: Stéphanie
authors: S
authors: Lemoine
authors: Deepak
authors: D
authors: Kalaskar
authors: Sophie
authors: S
authors: Demoustier-Champagne
authors: Hassan
authors: H
authors: Atmani
authors: Yves
authors: Y
authors: Mély
authors: Florence
authors: F
authors: Fioretti
authors: Guy
authors: G
authors: Schlatter
authors: Liisa
authors: L
authors: Kuhn
authors: Guy
authors: G
authors: Ladam
authors: Nadia
authors: N
authors: Benkirane-Jessel
mesh_terms: D000818:Animals; D040761:Biomimetic Materials; D055396:Bone Morphogenetic Protein 2 / Q000737:chemistry / Q000494:pharmacology*; D001861:Bone Regeneration / Q000502:physiology; D002130:Calcium Phosphates / Q000378:metabolism; D002478:Cells, Cultured; D048271:Chitosan / Q000737:chemistry*; D006801:Humans; D055767:Immobilized Proteins / Q000737:chemistry*; D051379:Mice; D008819:Mice, Nude; D008855:Microscopy, Electron, Scanning; D057139:Nanofibers / Q000737:chemistry; D010006:Osteoblasts; D010012:Osteogenesis / Q000187:drug effects; D053495:Osteopontin / Q000235:genetics; D011091:Polyesters / Q000737:chemistry; D011107:Polylysine; D012886:Skull / Q000166:cytology* / Q000502:physiology; D054457:Tissue Scaffolds
chemical_list: D055396:Bone Morphogenetic Protein 2; D002130:Calcium Phosphates; D055767:Immobilized Proteins; D011091:Polyesters; C506896:Spp1 protein, mouse; D053495:Osteopontin; C016240:polycaprolactone; D011107:Polylysine; D048271:Chitosan; C020243:calcium phosphate
keywords: biomimetic; bone morphogenetic protein; bone regeneration; layer-by-layer; poly(ϵ-caprolactone)