title: Graphene oxide-based fluorescent "on/off" switch for visual bioassay using "molecular beacon"-hosted Hoechst dyes.
abstract: In this study, the fluorescence of Hoechst dyes is significantly lit up by addition of our designed MB probe, forming a complex of "molecular beacon"-hosted Hoechst dyes (HMB). Combined with this property, a novel graphene oxide (GO)-based fluorescent "on/off" switch was developed to visually follow bioassay utilizing HMB as signal indicators and GO as an excellent energy acceptor to efficiently quench the fluorescence of HMB in a label-easy format. We have demonstrated its application for label-easy fluorescence "turn on" detection of sequence-specific DNA and "turn off" detection of exonuclease with sensitivity and selectivity in a single step in homogeneous solution. Compared to traditional molecular beacons, the proposed design is cost-effective and simple to prepare without fluorescence labeling or chemical modification.
authors: Lab of Biosystems and Microanalysis, State Key Laboratory of Bioreactor Engineering, East China University of Science & Technology , Meilong Road 130, Shanghai 200237, China.
authors: Min
authors: M
authors: Zhang
authors: Huynh-Nhu
authors: HN
authors: Le
authors: Bang-Ce
authors: BC
authors: Ye
mesh_terms: D001483:Base Sequence; D004247:DNA / Q000032:analysis; D057075:Enzyme Assays; D005092:Exonucleases / Q000378:metabolism; D005456:Fluorescent Dyes / Q000737:chemistry*; D006108:Graphite / Q000737:chemistry*; D010087:Oxides / Q000737:chemistry; D013050:Spectrometry, Fluorescence
chemical_list: D005456:Fluorescent Dyes; D010087:Oxides; D006108:Graphite; D004247:DNA; D005092:Exonucleases