title: Seroprevalence study of Equine rhinitis B virus (ERBV) in Australian weanling horses using serotype-specific ERBV enzyme-linked immunosorbent assays.
abstract: Respiratory infections are a major burden in the performance horse industry. Equine rhinitis B virus (ERBV) has been isolated from horses displaying clinical respiratory disease, and ERBV-neutralizing antibodies have been detected in 50-80% of horses in reported surveys. Current ERBV isolation and detection methods may underestimate the number of ERBV-positive animals and do not identify multiple serotype infections. The aim of the current study was to develop a serotyping ERBV antibody-detection enzyme-linked immunosorbent assay (ELISA) and examine the seroprevalence of ERBV in a group of Australian weanling horses. ELISAs with high sensitivity and specificity were developed. The seroprevalence of ERBV in the weanling horses was high (74-86%); ERBV-3 antibodies were most prevalent (58-62%) and ERBV-2 antibodies were least prevalent (10-16%). Many horses were seropositive to 2 or more serotypes. All 3 serotypes of ERBV were detected, and concurrent positivity to multiple serotypes was common.
authors: 1Carol A. Hartley, Centre for Equine Infectious Disease, Faculty of Veterinary Science, The University of Melbourne, Parkville, Victoria 3052, Australia. carolah@unimelb.edu.au.
authors: Jacquelyn
authors: J
authors: Horsington
authors: Carol A
authors: CA
authors: Hartley
authors: James R
authors: JR
authors: Gilkerson
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D001315:Australia / Q000453:epidemiology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000592:standards / Q000662:veterinary*; D052698:Erbovirus / Q000302:isolation & purification*; D006734:Horse Diseases / Q000097:blood / Q000453:epidemiology / Q000821:virology*; D006736:Horses; D009500:Neutralization Tests / Q000662:veterinary; D010850:Picornaviridae Infections / Q000097:blood / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D016036:Seroepidemiologic Studies
chemical_list: D000914:Antibodies, Viral
keywords: Equine rhinitis; seroprevalence