title: Adiponectin protein exists in aortic endothelial cells.
abstract: OBJECTIVE
  Inflammation is closely associated with the development of atherosclerosis and metabolic syndrome. Adiponectin, an adipose-derived secretory protein, possesses an anti-atherosclerotic property. The present study was undertaken to elucidate the presence and significance of adiponectin in vasculature.
  
  
  RESULTS
  Immunofluorescence staining was performed in aorta of wild-type (WT) mice and demonstrated that adiponectin was co-stained with CD31. Thoracic aorta was cut through and then aortic intima was carefully shaved from aorta. Western blotting showed the existence of adiponectin protein in aortic intima, while there was no adiponectin mRNA expression. Adiponectin knockout (Adipo-KO) and WT mice were administered with a low-dose and short-term lipopolysaccharide (LPS) (1 mg/kg of LPS for 4 hours). The endothelium vascular adhesion molecule-1 (VCAM-1) and intercellular adhesion molecule-1 (ICAM-1) were highly increased in Adipo-KO mice compared to WT mice after LPS administration.
  
  
  CONCLUSIONS
  Adiponectin protein exists in aortic endothelium under steady state and may protect vasculature from the initiation of atherosclerosis.
authors: Department of Metabolic Medicine, Graduate School of Medicine, Osaka University, Suita, Osaka, Japan.
authors: Noriyuki
authors: N
authors: Komura
authors: Norikazu
authors: N
authors: Maeda
authors: Takuya
authors: T
authors: Mori
authors: Shinji
authors: S
authors: Kihara
authors: Hideaki
authors: H
authors: Nakatsuji
authors: Ayumu
authors: A
authors: Hirata
authors: Yoshihiro
authors: Y
authors: Tochino
authors: Tohru
authors: T
authors: Funahashi
authors: Iichiro
authors: I
authors: Shimomura
mesh_terms: D052242:Adiponectin / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D001011:Aorta / Q000378:metabolism*; D015815:Cell Adhesion Molecules / Q000235:genetics / Q000378:metabolism; D042783:Endothelial Cells / Q000378:metabolism*; D004730:Endothelium, Vascular / Q000378:metabolism*; D007150:Immunohistochemistry; D008070:Lipopolysaccharides; D051379:Mice; D018345:Mice, Knockout; D017539:Tunica Intima / Q000378:metabolism
chemical_list: D052242:Adiponectin; D015815:Cell Adhesion Molecules; D008070:Lipopolysaccharides
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