title: Pneumocystis jirovecii haplotypes at the internal transcribed spacers of the rRNA operon in French HIV-negative patients with diverse clinical presentations of Pneumocystis infections.
abstract: Pneumocystis jirovecii, a transmissible fungus, is the causative agent of pulmonary infections. Its genomic diversity has appeared in reports from around the world but data on P. jirovecii genotypes in France are still limited. This study describes the typing of P. jirovecii isolates from 81 HIV-negative patients monitored at Brest University Hospital, Brittany, France, 40 of whom developed Pneumocystis pneumonia (PcP), and remaining 41 patients were colonized by the fungus. The isolates were assayed at the internal transcribed spacer (ITS)1 and ITS2 under improved amplification conditions to avoid in vitro ITS recombination. P. jirovecii ITS haplotypes were identified in 56/81 patients (31 PcP patients and 25 patients who were colonized) which revealed a high diversity in that 27 different haplotypes were identified. Eg was the most frequent haplotype (31/56, 55.3%), followed by Ec and Ai (5/56, 8.9% each). In contrast, Ne, usually the second most frequent haplotype in Europe and the USA, was observed in only 2/56 patients (3.6%). Mixed infections were detected in 18/56 patients (32.1%; 12 PcP patients and six who were colonized). No significant differences were observed in haplotype diversity, frequency of peculiar haplotypes, and mixed infection occurrence, between the two patient populations. The study, conducted with the largest HIV-negative patient population investigated so far, shows that ITS typing remains an efficient method for characterizing P. jirovecii among human populations, whatever their clinical presentation of Pneumocystis infections.
authors: * University of Brest , LUBEM EA 3882, Brest.
authors: Solene
authors: S
authors: Le Gal
authors: Amelie
authors: A
authors: Rouille
authors: Paul
authors: P
authors: Gueguen
authors: Michele
authors: M
authors: Virmaux
authors: Christian
authors: C
authors: Berthou
authors: Gaëlle
authors: G
authors: Guillerm
authors: Francis
authors: F
authors: Couturaud
authors: Yann
authors: Y
authors: Le Meur
authors: Celine
authors: C
authors: Damiani
authors: Anne
authors: A
authors: Totet
authors: Gilles
authors: G
authors: Nevez
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002648:Child; D002675:Child, Preschool; D021903:DNA, Ribosomal Spacer / Q000235:genetics*; D005260:Female; D005602:France / Q000453:epidemiology; D014644:Genetic Variation; D006239:Haplotypes; D006801:Humans; D008297:Male; D008875:Middle Aged; D017720:Molecular Epidemiology; D016720:Pneumocystis Infections / Q000453:epidemiology / Q000382:microbiology*; D045363:Pneumocystis carinii / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D055815:Young Adult
chemical_list: D021903:DNA, Ribosomal Spacer