title: Autophosphorylation of gatekeeper tyrosine by symbiosis receptor kinase.
abstract: Plant receptor-like kinases (RLKs) share their evolutionary origin with animal interleukin-1 receptor-associated kinase (IRAK)/Pelle family of soluble kinases and are distinguished by having tyrosine as 'gatekeeper'. This position is adjacent to the hinge region and is hidden in a hydrophobic pocket of the catalytic cleft of protein kinases and is therefore least probable to be a target for any modification. This communication illustrates the accessibility of the gatekeeper site (Y670) towards both autophosphorylation and dephosphorylation in the recombinant cytoplasmic domain of symbiosis receptor kinase from Arachis hypogaea (AhSYMRK). Autophosphorylation on gatekeeper tyrosine was detected prior to extraction but never under in vitro conditions. We hypothesize gatekeeper phosphorylation to be associated with synthesis/maturation of AhSYMRK and this phenomenon may be prevalent among RLKs.
authors: Department of Biochemistry, University of Calcutta, Kolkata, India.
authors: Sandip
authors: S
authors: Samaddar
authors: Ayan
authors: A
authors: Dutta
authors: Senjuti
authors: S
authors: Sinharoy
authors: Anindita
authors: A
authors: Paul
authors: Avisek
authors: A
authors: Bhattacharya
authors: Sudip
authors: S
authors: Saha
authors: Ko-yi
authors: KY
authors: Chien
authors: Michael B
authors: MB
authors: Goshe
authors: Maitrayee
authors: M
authors: DasGupta
mesh_terms: D020816:Amino Acid Motifs; D010367:Arachis / Q000235:genetics / Q000378:metabolism*; D020134:Catalytic Domain; D002460:Cell Line; D018506:Gene Expression Regulation, Plant; D009154:Mutation; D010766:Phosphorylation; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D020794:Receptor Protein-Tyrosine Kinases / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction; D014443:Tyrosine / Q000235:genetics / Q000378:metabolism*
chemical_list: D010940:Plant Proteins; D011994:Recombinant Proteins; D014443:Tyrosine; D020794:Receptor Protein-Tyrosine Kinases
keywords: Autophosphorylation; CD; CIAP; Gatekeeper residue; IRAK; KD; RLK; Receptor-like kinase; SYMRK; Symbiosis receptor kinase; TKL; Trx; Tyrosine kinase-like; calf intestinal alkaline phosphatase; cytoplasmic domain; interleukin-1 receptor-associated kinase; kinase domain; receptor-like kinase; symbiosis receptor kinase; thioredoxin; tyrosine-kinase like