title: Increased collagen accumulation in eggshell membrane after feeding with dietary wood charcoal powder and vinegar.
abstract: Collagen in an eggshell membrane is important for egg preservation, medical burn treatment and manufacturing of cosmetics. Because collagen in the membrane is little, it is a need to improve the accumulation in the membrane to develop these applications. Wood charcoal powder with vinegar (WCV) is a natural substance that improves poultry production. In hen fed with WCV, total collagen in the eggshell membrane increased with an increase in dietary WCV and significantly increased in the 1.0% WCV group (p < 0.05). Scanning and light microscopic images revealed that this group had thicker eggshell membranes and a fine mesh structure composed of finer and more densely distributed fibres than in the control. Eggs from WCV group showed slow Haugh unit decrease during egg storage and the decrease correlated with total collagen in eggshell membrane. In intact chicken, type I and type III collagens were found in different specific locations in the oviduct but not in the membrane. The finding that collagen accumulates in the eggshell membrane under WCV feeding suggests that feeding chicken with WCV will permit long-term storage of eggs in poultry production, and the increased volume of total collagen will facilitate its application in medicine and cosmetics.
authors: Miyazaki Midori Pharms. Inc. , Miyazaki , Japan .
authors: Kohsyo
authors: K
authors: Yamauchi
authors: Noboru
authors: N
authors: Manabe
authors: Yoshiki
authors: Y
authors: Matsumoto
authors: Koh-En
authors: KE
authors: Yamauchi
mesh_terms: D019342:Acetic Acid / Q000494:pharmacology*; D000818:Animals; D015153:Blotting, Western; D002606:Charcoal / Q000494:pharmacology*; D002645:Chickens / Q000378:metabolism*; D024042:Collagen Type I / Q000378:metabolism*; D024061:Collagen Type III / Q000378:metabolism*; D004032:Diet / Q000662:veterinary*; D004528:Egg Shell / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D005260:Female; D005455:Fluorescent Antibody Technique; D008566:Membranes / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D010057:Oviducts / Q000187:drug effects / Q000378:metabolism; D011208:Powders; D014934:Wood / Q000737:chemistry*
chemical_list: D024042:Collagen Type I; D024061:Collagen Type III; D011208:Powders; D002606:Charcoal; D019342:Acetic Acid