title: A pair of interneurons influences the choice between feeding and locomotion in Drosophila.
abstract: The decision to engage in one behavior often precludes the selection of others, suggesting cross-inhibition between incompatible behaviors. For example, the likelihood to initiate feeding might be influenced by an animal's commitment to other behaviors. Here, we examine the modulation of feeding behavior in the fruit fly, Drosophila melanogaster, and identify a pair of interneurons in the ventral nerve cord that is activated by stimulation of mechanosensory neurons and inhibits feeding initiation, suggesting that these neurons suppress feeding while the fly is walking. Conversely, inhibiting activity in these neurons promotes feeding initiation and inhibits locomotion. These studies demonstrate the mutual exclusivity between locomotion and feeding initiation in the fly, isolate interneurons that influence this behavioral choice, and provide a framework for studying the neural basis for behavioral exclusivity in Drosophila.
authors: Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA.
authors: Kevin
authors: K
authors: Mann
authors: Michael D
authors: MD
authors: Gordon
authors: Kristin
authors: K
authors: Scott
mesh_terms: D000200:Action Potentials / Q000187:drug effects / Q000235:genetics; D000704:Analysis of Variance; D000818:Animals; D030801:Animals, Genetically Modified; D001921:Brain / Q000166:cytology; D002147:Calmodulin / Q000235:genetics / Q000378:metabolism; D002755:Choice Behavior / Q000502:physiology*; D004305:Dose-Response Relationship, Drug; D004330:Drosophila; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism; D005247:Feeding Behavior / Q000187:drug effects / Q000502:physiology*; D005508:Food Deprivation; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D007395:Interneurons / Q000145:classification / Q000502:physiology*; D008124:Locomotion / Q000187:drug effects / Q000502:physiology*; D009219:Myosin-Light-Chain Kinase / Q000235:genetics / Q000378:metabolism; D010446:Peptide Fragments / Q000235:genetics / Q000378:metabolism; D012243:Rhodopsin / Q000235:genetics; D013395:Sucrose / Q000008:administration & dosage; D013549:Sweetening Agents / Q000008:administration & dosage; D013696:Temperature; D014760:Viral Fusion Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D002147:Calmodulin; D029721:Drosophila Proteins; C107660:M13 protein (myosin light-chain kinase); D010446:Peptide Fragments; D013549:Sweetening Agents; D014760:Viral Fusion Proteins; D049452:Green Fluorescent Proteins; D013395:Sucrose; D012243:Rhodopsin; D009219:Myosin-Light-Chain Kinase
references: Nature. 2013 Jan 10;493(7431):221-5
references: 23222543
references: Genetics. 2010 Oct;186(2):735-55
references: 20697123
references: J Neurosci. 2010 May 5;30(18):6247-52
references: 20445050
references: Cell. 2003 Apr 18;113(2):261-73
references: 12705873
references: Nat Methods. 2009 Dec;6(12):875-81
references: 19898485
references: J Neurosci. 2012 Oct 3;32(40):13819-40
references: 23035093
references: Genesis. 2002 Sep-Oct;34(1-2):142-5
references: 12324970
references: Cell. 2012 Feb 3;148(3):583-95
references: 22304923
references: Proc Natl Acad Sci U S A. 2010 Nov 23;107(47):20553-8
references: 21059961
references: J Neurosci. 2001 Mar 1;21(5):1523-31
references: 11222642
references: Cell. 2007 Aug 24;130(4):717-29
references: 17719548
references: J Neurobiol. 2001 May;47(2):81-92
references: 11291099
references: Neuron. 1999 Mar;22(3):451-61
references: 10197526
references: Nature. 2010 Dec 2;468(7324):686-90
references: 21124455
references: J Exp Biol. 1994 Dec;197:215-35
references: 7852903
references: Nature. 2010 May 6;465(7294):91-5
references: 20364123
references: Proc Natl Acad Sci U S A. 2003 Nov 25;100(24):13940-5
references: 14615590
references: Neuron. 2009 Feb 12;61(3):373-84
references: 19217375
references: Cell. 2003 Jan 24;112(2):271-82
references: 12553914
references: Neuron. 2006 Jan 19;49(2):285-95
references: 16423701
references: Cell. 2012 May 25;149(5):1140-51
references: 22632976
references: Nat Methods. 2009 Jun;6(6):451-7
references: 19412169
references: Neuron. 2012 Mar 8;73(5):941-50
references: 22405204
references: Proc Natl Acad Sci U S A. 2012 Apr 17;109(16):6307-12
references: 22474379
references: Curr Biol. 2004 Jun 22;14(12):1065-79
references: 15202999
references: Cell Tissue Res. 1994 Jan;275(1):3-26
references: 8118845
references: Cell. 2010 Apr 30;141(3):536-48
references: 20434990
references: PLoS One. 2011;6(9):e24531
references: 21931740
references: Nature. 2008 Jul 10;454(7201):217-20
references: 18548007
references: J Neurosci. 2006 Oct 18;26(42):10925-33
references: 17050731
references: Cell. 2004 Jun 25;117(7):981-91
references: 15210117
references: Sci STKE. 2004 Feb 12;2004(220):pl6
references: 14970377
references: J Neurophysiol. 1999 Feb;81(2):654-67
references: 10036268
references: Biol Cybern. 1992;66(6):485-96
references: 1586673
references: Nat Neurosci. 2009 Nov;12(11):1450-7
references: 19801989
references: Science. 2005 Feb 11;307(5711):896-901
references: 15705844
references: Neuron. 2010 Aug 12;67(3):373-80
references: 20696376
references: Cell Rep. 2012 Jun 28;1(6):599-607
references: 22813735
references: Proc Natl Acad Sci U S A. 2010 Aug 17;107(33):14833-8
references: 20679196
references: J Neurosci. 2010 Jul 21;30(29):9753-61
references: 20660257
references: PLoS Genet. 2012;8(3):e1002587
references: 22438833
references: J Neurophysiol. 1995 Nov;74(5):1900-10
references: 8592183
references: Nat Methods. 2011 Mar;8(3):231-7
references: 21473015