title: Ag+-mediated DNA base pairing: extraordinarily stable pyrrolo-dC-pyrrolo-dC pairs binding two silver ions.
abstract: 6-Substituted pyrrolo-dC-pyrrolo-dC mismatches selectively capture silver ions to form extraordinarily stable metal-mediated base pairs. One single modification in a 12-mer duplex causes a Tm increase of 36.0 °C relative to the metal-free mismatched duplex. Spectrophotometric titrations as well as ESI mass spectra confirmed the binding of two silver ions per base pair. The Ag(+)-mediated base pairs may permit the construction of metal-responsive DNA with a very high silver loading.
authors: Laboratory of Bioorganic Chemistry and Chemical Biology, Center for Nanotechnology , Heisenbergstraße 11, 48149 Münster, Germany.
authors: Hui
authors: H
authors: Mei
authors: Ingo
authors: I
authors: Röhl
authors: Frank
authors: F
authors: Seela
mesh_terms: D020029:Base Pairing; D004247:DNA / Q000737:chemistry*; D007477:Ions / Q000737:chemistry; D015394:Molecular Structure; D011758:Pyrroles / Q000737:chemistry*; D012834:Silver / Q000737:chemistry*
chemical_list: D007477:Ions; D011758:Pyrroles; D012834:Silver; D004247:DNA