title: Isolation and characterization of a novel ε-caprolactam-degrading microbe, Acinetobacter calcoaceticus, from industrial wastewater by chemostat-enrichment.
abstract: For the isolation of a ε-caprolactam-degrading microbe from wastewaters of a factory producing caprolactam, we applied a chemostat-enrichment technique with a selective medium containing caprolactam as sole source of carbon and nitrogen. This allowed for the isolation of a novel caprolactam-degrading microbe, identified as Acinetobacter calcoaceticus. The strain had a critical tolerance of 19 g caprolactam l(-1) in minimal medium, which is higher than any previously reported caprolactam-degrading microbe. A. calcoaceticus also decreased the caprolactam content in medium by 65 % within 72 h despite the high caprolactam content (10 g l(-1)). This study highlights the potential use of A. calcoaceticus strain for the bioremediation of recalcitrant synthetic polymers, such as caprolactam.
authors: Biotechnology Process Engineering Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 52 Oun-dong, Yusong, Daejeon, 305-806, Republic of Korea, sasi85@kribb.re.kr.
authors: Sasikumar
authors: S
authors: Rajoo
authors: Jung Oh
authors: JO
authors: Ahn
authors: Hong Weon
authors: HW
authors: Lee
authors: Joon Ki
authors: JK
authors: Jung
mesh_terms: D016954:Acinetobacter calcoaceticus / Q000187:drug effects / Q000302:isolation & purification* / Q000378:metabolism; D001673:Biodegradation, Environmental; D002209:Caprolactam / Q000378:metabolism* / Q000494:pharmacology; D049109:Cell Proliferation / Q000187:drug effects; D007220:Industrial Waste; D062065:Wastewater / Q000382:microbiology*
chemical_list: D002209:Caprolactam; D007220:Industrial Waste; D062065:Wastewater