title: Temporal changes and altitudinal distribution of aerobic anoxygenic phototrophs in mountain lakes.
abstract: Aerobic anoxygenic phototrophs (AAPs) are bacteriochlorophyll a-containing microorganisms that use organic substrates for growth but can supplement their energy requirements with light. They have been reported from various marine and limnic environments; however, their ecology remains largely unknown. Here infrared epifluorescence microscopy was used to monitor temporal changes in AAPs in the alpine lake Gossenköllesee, located in the Tyrolean Alps, Austria. AAP abundance was low (10(3) cells ml(-1)) until mid-July and reached a maximum of ∼1.3 × 10(5) cells ml(-1) (29% of all prokaryotes) in mid-September. We compared the studied lake with other mountain lakes located across an altitudinal gradient (913 to 2,799 m above sea level). The concentration of dissolved organic carbon and water transparency seem to be the main factors influencing AAP abundance during the seasonal cycle as well as across the altitudinal gradient. While the AAP populations inhabiting the alpine lakes were composed of intensely pigmented large rods (5 to 12 μm), the lakes below the tree line were inhabited by a variety of smaller morphotypes. Analysis of pufM diversity revealed that AAPs in Gossenköllesee were almost exclusively Sphingomonadales species, which indicates that AAP communities inhabiting alpine lakes are relatively homogeneous compared to those in low-altitude lakes.
authors: Institute of Microbiology CAS, Opatovický mlýn, Třeboň, Czech Republic.
authors: Zuzana
authors: Z
authors: Čuperová
authors: Evelyn
authors: E
authors: Holzer
authors: Ivette
authors: I
authors: Salka
authors: Ruben
authors: R
authors: Sommaruga
authors: Michal
authors: M
authors: Koblízek
mesh_terms: D020561:Alphaproteobacteria / Q000235:genetics / Q000302:isolation & purification; D000693:Anaerobiosis; D001317:Austria; D044822:Biodiversity; D055598:Chemical Phenomena; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D060106:Lakes / Q000382:microbiology*; D008969:Molecular Sequence Data; D009930:Organic Chemicals / Q000032:analysis; D052817:Phototrophic Processes; D010802:Phylogeny; D012621:Seasons; D017422:Sequence Analysis, DNA; D014867:Water / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D009930:Organic Chemicals; D014867:Water
references: Photochem Photobiol Sci. 2009 Sep;8(9):1244-56
references: 19707613
references: Appl Environ Microbiol. 2009 Dec;75(23):7537-41
references: 19801464
references: Nature. 2002 Feb 7;415(6872):630-3
references: 11832943
references: Limnol Oceanogr. 2010 May;55(3):1134-1144
references: 20401318
references: Microbiol Immunol. 2000;44(7):563-75
references: 10981829
references: ISME J. 2012 Jan;6(1):113-23
references: 21716306
references: Environ Microbiol. 2007 Dec;9(12):3091-9
references: 17991036
references: Science. 2001 Jun 29;292(5526):2492-5
references: 11431568
references: Environ Microbiol. 2007 Jun;9(6):1464-75
references: 17504484
references: ISME J. 2010 Jul;4(7):945-54
references: 20182527
references: Appl Environ Microbiol. 2006 Jan;72(1):557-64
references: 16391092
references: J Photochem Photobiol B. 2001 Sep 1;62(1-2):35-42
references: 11693365
references: Nature. 2000 Sep 14;407(6801):177-9
references: 11001053
references: Environ Microbiol. 2008 Aug;10(8):1988-96
references: 18430010
references: Appl Environ Microbiol. 2009 Nov;75(22):7221-8
references: 19801478
references: Environ Microbiol. 2011 Nov;13(11):2865-75
references: 21895915
references: Int J Syst Evol Microbiol. 2006 Apr;56(Pt 4):847-854
references: 16585705
references: Appl Environ Microbiol. 2008 Jul;74(13):4012-21
references: 18469118
references: Environ Microbiol. 2011 Oct;13(10):2717-25
references: 21883792
references: Appl Environ Microbiol. 2004 Nov;70(11):6542-50
references: 15528517
references: Environ Microbiol. 2005 Dec;7(12):1896-908
references: 16309388
references: Int J Syst Bacteriol. 1993 Jan;43(1):125-34
references: 8427804
references: Int J Syst Bacteriol. 1999 Apr;49 Pt 2:449-57
references: 10319464
references: FEMS Microbiol Ecol. 2005 Feb 1;51(3):353-61
references: 16329883
references: Appl Environ Microbiol. 2012 Oct;78(20):7414-9
references: 22885759
references: Appl Environ Microbiol. 1999 Sep;65(9):3820-7
references: 10473381