title: Developmental regulation of linkers of the nucleoskeleton to the cytoskeleton during mouse postnatal retinogenesis.
abstract: Sun proteins and Nesprins are two families of proteins whose direct interactions across the nuclear envelope provide for the core of Linkers of the Nucleoskeleton to the Cytoskeleton (LINC complexes) that physically connect the nucleus interior to cytoskeletal networks. Whereas LINC complexes play essential roles in nuclear migration anchorage and underlie normal CNS development, the developmental regulation of their composition remains largely unknown. In this study, we examined the spatiotemporal expression of lamins, Sun proteins and Nesprins during postnatal mouse retinal development. Whereas retinal precursor cells mostly express B-type lamins, Sun1, and high molecular weight isoforms of Nesprins, post-mitotic retinal cells are characterized by a drastic downregulation of the latter, the expression of A-type lamins, and the strong induction of a specific isoform of Nesprin1 late in retinal development. Importantly, our results emphasize different spatiotemporal expression for Nesprin1 and Nesprin2 and further suggest an important role for KASH-less isoforms of Nesprin1 in the CNS. In conclusion, the transition from retinal precursor cells undergoing interkinetic nuclear migration to post-mitotic retinal cells undergoing nuclear translocation and/or anchorage is accompanied by a profound remodeling of LINC complexes composition. This remodeling may reflect different requirements of nuclear dynamics at different stages of CNS development.
authors: Department of Ophthalmology and Visual Sciences; Washington University School of Medicine; St Louis, MO USA; Division of Molecular Genetics; German Cancer Research Center (DKFZ); Heidelberg, Germany.
authors: David S
authors: DS
authors: Razafsky
authors: Candace L
authors: CL
authors: Ward
authors: Thorsten
authors: T
authors: Kolb
authors: Didier
authors: D
authors: Hodzic
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003598:Cytoskeletal Proteins; D003599:Cytoskeleton / Q000235:genetics / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental; D008565:Membrane Proteins / Q000235:genetics; D051379:Mice; D008869:Microtubule-Associated Proteins / Q000235:genetics; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000737:chemistry / Q000235:genetics; D015530:Nuclear Matrix / Q000235:genetics / Q000378:metabolism*; D009687:Nuclear Proteins / Q000737:chemistry / Q000235:genetics; D012160:Retina / Q000166:cytology / Q000254:growth & development*; D034501:Telomere-Binding Proteins / Q000235:genetics
chemical_list: D003598:Cytoskeletal Proteins; D008565:Membrane Proteins; D008869:Microtubule-Associated Proteins; D009419:Nerve Tissue Proteins; D009687:Nuclear Proteins; C464932:SUN1 protein, mouse; C520338:Sun2 protein, mouse; C485901:Syne1 protein, mouse; C485903:Syne2 protein, mouse; D034501:Telomere-Binding Proteins
keywords: KASH domain; Nesprins; Sun proteins; lamins; nuclear envelope; retinogenesis
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