title: Expression of aquaporins in prostate and seminal vesicles of diabetic rats.
abstract: BACKGROUND
  Aquaporins (AQPs) are membrane proteins that facilitate the movement of water and many small solutes across biological membranes. Seminal fluid is primarily produced by prostate and seminal vesicles, and its production may potentially be mediated by many mechanisms related to transudation of fluid. Epidemiological data suggest that semen volume in diabetic men is significantly less than in nondiabetic men.
  
  
  OBJECTIVE
  To investigate the change in volume of secretions of the prostate and seminal vesicles in diabetic rats and its association with the expression of AQPs 1-4.
  
  
  METHODS
  Twenty male Sprague Dawley rats were randomly divided among 4- and 6-week diabetic groups and 4- and 6-week control groups. Prostate and seminal vesicle secretions were collected and measured, and levels of expression of AQPs 1-4 were determined by immunohistochemical study and Western blot.
  
  
  METHODS
  The levels of expression of AQPs 1-4 were determined in the prostate and seminal vesicles of diabetic rats by Western blot and immunohistochemical study.
  
  
  RESULTS
  Plasma glucose was significantly higher in diabetic model groups than in controls (P < 0.05). The weights of secretions of the prostate and seminal vesicles were significantly lower in diabetic model groups (P < 0.05). The levels of expression of AQPs 1 and 4 in seminal vesicles were significantly lower in diabetic model groups (P < 0.05). There was no difference in the level of expression of AQP3 in seminal vesicles among the groups. The levels of expression of AQPs 1, 3, and 4 in prostate were significantly lower in diabetic model groups (P < 0.05). AQP2 was not detectable in the prostate or seminal vesicles of any of the groups.
  
  
  CONCLUSIONS
  Decreased weight of prostate secretions in diabetic rats may be partly due to decreased levels of AQPs 1, 3, and 4 in prostatic tissue. Decreased weight of seminal vesicle secretions in diabetic rats may be partly due to decreased levels of AQP1 and AQP4 in seminal vesicles. There is no relationship between the expression of AQPs 1-4 and the duration of disease.
authors: Department of Nuclear Medicine, Affiliated Hospital, Luzhou Medical College, Luzhou, China.
authors: Lijun
authors: L
authors: Pei
authors: Guosheng
authors: G
authors: Yang
authors: Jun
authors: J
authors: Jiang
authors: Rui
authors: R
authors: Jiang
authors: Qingfu
authors: Q
authors: Deng
authors: Bo
authors: B
authors: Chen
authors: Xilun
authors: X
authors: Gan
mesh_terms: D000818:Animals; D020346:Aquaporins / Q000378:metabolism*; D015153:Blotting, Western; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism*; D006801:Humans; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism; D011467:Prostate / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012669:Seminal Vesicles / Q000378:metabolism*
chemical_list: D020346:Aquaporins; D008565:Membrane Proteins
keywords: Aquaporins; Diabetes Mellitus; Prostate; Rat; Seminal Vesicles; Water Channel