title: Dynamic localization and functional implications of C-peptide might for suppression of iNOS in high glucose-stimulated rat mesangial cells.
abstract: Although C-peptide has unique beneficial effects on diabetic nephropathy (DN), its functional localization and molecular mechanism have not been fully clarified. Whether C-peptide exhibits its protective role through the regulation of inducible nitric oxide synthase (iNOS), a key enzyme in oxidative stress, is not clear. In this study, it was revealed that C-peptide could enter the nucleus of high glucose-stimulated mesangial cells, especially in a time-dependent manner by high glucose pretreatment, while no C-peptide was detected in low glucose-cultured mesangial cells. The dynamic functional localization of C-peptide might be the intrinsic cause of its unique beneficial effects for DN, which may provide a foundation for further clarification of its underlying mechanism. Our preliminarily data also shown C-peptide suppressed the iNOS expression. Taking together, these results revealed the dynamic functional localization of C-peptide by high glucose stimulation in rat mesangial cells, which might suppress expression of iNOS to exhibit its protective effects.
authors: Department of Molecular Biology, Hebei Key Lab of Laboratory Animal, Hebei Medical University, Shijiazhuang, China.
authors: Yanning
authors: Y
authors: Li
authors: Mingming
authors: M
authors: Zhao
authors: Bin
authors: B
authors: Li
authors: Jinsheng
authors: J
authors: Qi
mesh_terms: D000818:Animals; D002096:C-Peptide / Q000502:physiology*; D002467:Cell Nucleus / Q000378:metabolism; D002478:Cells, Cultured; D004790:Enzyme Induction; D005947:Glucose / Q000502:physiology*; D050527:Mesangial Cells / Q000201:enzymology*; D016328:NF-kappa B / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II / Q000235:genetics / Q000378:metabolism*; D021381:Protein Transport; D051381:Rats; D015533:Transcriptional Activation
chemical_list: D002096:C-Peptide; D016328:NF-kappa B; D052247:Nitric Oxide Synthase Type II; C496318:Nos2 protein, rat; D005947:Glucose
keywords: C-peptide; DMEM; DN; Diabetic nephropathy; Dulbecco’s modified Eagle’s medium; HG; Inducible nitric oxide synthase; LG; NF-κB; high glucose; iNOS; inducible nitric oxide synthase; low glucose; nuclear factor-kappa B