title: Tenascin and fibronectin expression after pulp capping with different hemostatic agents: a preliminary study.
abstract: This study investigated the expression of extracellular matrix glycoproteins tenascin (TN) and fibronectin (FN) in pulp repair after capping with calcium hydroxide (CH), following different hemostasis protocols. Class I cavities with a pulp exposure were prepared in 42 human third molars scheduled for extraction. Different hemostatic agents (0.9% saline solution, 5.25% sodium hypochlorite and 2% chlorhexidine digluconate) were used and pulps were capped with CH cement. After 7, 30 or 90 days, teeth were extracted, formalin-fixed, and prepared for immunohistochemical technique. Hemostatic agents did not influence the expression of TN and FN. Both glycoproteins were found in the entire the pulp tissue and around collagen fibers, but were absent in the mineralized tissues. In the predentin, TN showed positive immunostaining and FN had a variable expression. Within 7 days post-treatment, a slightly more pronounced immunostaining on the pulp exposure site was observed. Within 30 days, TN and FN demonstrated a positive expression around the dentin barrier and at 90 days, a thin and linear expression of TN and FN was delimitating the reparative dentin. In conclusion, hemostatic agents did not influence TN and FN expression. Immunostaining for TN and FN was seen in different regions and periods, demonstrating their role in pulp repair.
authors: Graduate Program in Dentistry, Federal University of Pelotas, Pelotas, RS, Brazil.
authors: Elaine Zanchin
authors: EZ
authors: Baldissera
authors: Adriana Fernandes da
authors: AF
authors: Silva
authors: Ana Paula Neutzling
authors: AP
authors: Gomes
authors: Adriana
authors: A
authors: Etges
authors: Tatiana
authors: T
authors: Botero
authors: Flávio Fernando
authors: FF
authors: Demarco
authors: Sandra Beatriz Chaves
authors: SB
authors: Tarquinio
mesh_terms: D000328:Adult; D017438:Bisphenol A-Glycidyl Methacrylate / Q000737:chemistry; D002126:Calcium Hydroxide / Q000627:therapeutic use; D002710:Chlorhexidine / Q000031:analogs & derivatives / Q000627:therapeutic use; D003094:Collagen / Q000032:analysis; D003188:Composite Resins / Q000737:chemistry; D003782:Dental Pulp / Q000737:chemistry; D003785:Dental Pulp Capping; D003789:Dental Pulp Exposure / Q000628:therapy; D003793:Dental Restoration, Permanent / Q000379:methods; D003804:Dentin / Q000737:chemistry; D003809:Dentin, Secondary / Q000737:chemistry; D005353:Fibronectins / Q000032:analysis*; D005500:Follow-Up Studies; D006490:Hemostatics / Q000627:therapeutic use*; D006801:Humans; D057872:Pulp Capping and Pulpectomy Agents / Q000627:therapeutic use*; D012965:Sodium Chloride / Q000627:therapeutic use; D012973:Sodium Hypochlorite / Q000627:therapeutic use; D019063:Tenascin / Q000032:analysis*; D014081:Tooth Extraction; D055815:Young Adult
chemical_list: D003188:Composite Resins; D005353:Fibronectins; C444701:Filtek Z250; D006490:Hemostatics; D057872:Pulp Capping and Pulpectomy Agents; D019063:Tenascin; C115612:single bond; D012965:Sodium Chloride; D017438:Bisphenol A-Glycidyl Methacrylate; D003094:Collagen; D012973:Sodium Hypochlorite; C010882:chlorhexidine gluconate; D002126:Calcium Hydroxide; D002710:Chlorhexidine