title: MicroRNA expression profiles in avian haemopoietic cells.
abstract: MicroRNAs (miRNAs) are small, abundant, non-coding RNAs that modulate gene expression by interfering with translation or stability of mRNA transcripts in a sequence-specific manner. A total of 734 precursor and 996 mature miRNAs have so far been identified in the chicken genome. A number of these miRNAs are expressed in a cell type-specific manner, and understanding their function requires detailed examination of their expression in different cell types. We carried out deep sequencing of small RNA populations isolated from stimulated or transformed avian haemopoietic cell lines to determine the changes in the expression profiles of these important regulatory molecules during these biological events. There were significant changes in the expression of a number of miRNAs, including miR-155, in chicken B cells stimulated with CD40 ligand. Similarly, avian leukosis virus (ALV)-transformed DT40 cells also showed changes in miRNA expression in relation to the naïve cells. Embryonic stem cell line BP25 demonstrated a distinct cluster of upregulated miRNAs, many of which were shown previously to be involved in embryonic stem cell development. Finally, chicken macrophage cell line HD11 showed changes in miRNA profiles, some of which are thought to be related to the transformation by v-myc transduced by the virus. This work represents the first publication of a catalog of microRNA expression in a range of important avian cells and provides insights into the potential roles of miRNAs in the hematopoietic lineages of cells in a model non-mammalian species.
authors: Avian Viral Diseases Programme, Compton Laboratory, The Pirbright Institute Berkshire, UK.
authors: Yongxiu
authors: Y
authors: Yao
authors: Jane
authors: J
authors: Charlesworth
authors: Venugopal
authors: V
authors: Nair
authors: Mick
authors: M
authors: Watson
keywords: B-cell; DT40; HD11; IAH30; macrophages; microRNA
references: Leuk Res. 2006 May;30(5):643-7
references: 16226311
references: J Gen Virol. 2009 Jul;90(Pt 7):1551-1559
references: 19297609
references: Blood. 2013 Feb 7;121(6):984-95
references: 23144169
references: Oncology. 2007;72(5-6):397-402
references: 18196926
references: Cell Res. 2010 Oct;20(10):1158-69
references: 20856265
references: Pancreas. 2011 Nov;40(8):1180-7
references: 21785383
references: Front Biosci (Landmark Ed). 2012 Jun 01;17:2350-5
references: 22652783
references: J Gen Virol. 2012 Jul;93(Pt 7):1530-1536
references: 22492913
references: Asian Pac J Cancer Prev. 2011;12(11):3001-4
references: 22393979
references: Science. 1984 Jun 22;224(4655):1312-6
references: 6427923
references: Vet Immunol Immunopathol. 1996 Dec;55(1-3):225-34
references: 9014319
references: PLoS Biol. 2010 Sep 07;8(9):
references: 20838655
references: Histol Histopathol. 2010 Apr;25(4):505-13
references: 20183803
references: Dev Comp Immunol. 2008;32(9):1015-26
references: 18374414
references: Cell. 1979 Oct;18(2):375-90
references: 227607
references: Genome Res. 2008 Jun;18(6):957-64
references: 18469162
references: Clin Cancer Res. 2008 Jan 15;14(2):419-27
references: 18223217
references: Breast Cancer Res Treat. 2012 Aug;134(3):1081-93
references: 22476851
references: Nat Biotechnol. 2008 Apr;26(4):407-15
references: 18392026
references: Leuk Res. 2012 Mar;36(3):293-8
references: 22099053
references: Dev Comp Immunol. 2005;29(4):361-74
references: 15859239
references: Dev Comp Immunol. 2001 Jan;25(1):69-82
references: 10980321
references: J Virol. 2009 Dec;83(23):12009-17
references: 19759154
references: Cancer Cell. 2011 Jul 12;20(1):104-18
references: 21741600
references: Biochem Soc Trans. 2008 Dec;36(Pt 6):1211-5
references: 19021527
references: Cell. 2000 Sep 1;102(5):553-63
references: 11007474
references: Nucleic Acids Res. 2002 Jan 1;30(1):230-1
references: 11752301
references: Cytokine Growth Factor Rev. 2011 Jun;22(3):141-7
references: 21703910
references: Eur J Cancer. 2011 Sep;47(14):2166-74
references: 21636267
references: J Virol. 2001 May;75(9):4219-25
references: 11287571
references: BMC Genomics. 2008 Apr 22;9:185
references: 18430245
references: Mol Cell Biol. 1989 Jun;9(6):2657-64
references: 2548084
references: Cytogenet Genome Res. 2007;117(1-4):189-94
references: 17675859
references: Nat Methods. 2008 Jul;5(7):621-8
references: 18516045
references: Dev Biol. 2004 Jun 15;270(2):488-98
references: 15183728
references: Mol Immunol. 2010 Apr;47(7-8):1383-95
references: 20299102
references: Curr Stem Cell Res Ther. 2009 Sep;4(3):168-77
references: 19492978
references: Eur J Haematol. 2008 Oct;81(4):304-10
references: 18573170
references: J Transl Med. 2009 Mar 23;7:20
references: 19309508
references: Biochim Biophys Acta. 2011 Nov-Dec;1809(11-12):654-9
references: 21683170
references: J Hematol Oncol. 2012 Mar 27;5:13
references: 22453030
references: J Cell Mol Med. 2009 Jan;13(1):39-53
references: 19175699
references: Cancer Cell Int. 2010 May 04;10:15
references: 20441582
references: Nature. 2010 Mar 11;464(7286):237-242
references: 20220842
references: J Virol. 2008 Apr;82(8):4007-15
references: 18256158
references: Proc Natl Acad Sci U S A. 2007 Nov 13;104(46):18097-102
references: 17989215
references: Carcinogenesis. 2012 Apr;33(4):732-9
references: 22287560
references: Development. 1996 Aug;122(8):2339-48
references: 8756279
references: Proc Natl Acad Sci U S A. 2011 Jun 28;108(26):10426-31
references: 21670268
references: J Biol Chem. 2011 Jan 21;286(3):1675-82
references: 20947507