title: RNase III is required for actinomycin production in Streptomyces antibioticus.
abstract: Using insertional mutagenesis, we have disrupted the RNase III gene, rnc, of the actinomycin-producing streptomycete, Streptomyces antibioticus. Disruption was verified by Southern blotting. The resulting strain grows more vigorously than its parent on actinomycin production medium but produces significantly lower levels of actinomycin. Complementation of the rnc disruption with the wild-type rnc gene from S. antibioticus restored actinomycin production to nearly wild-type levels. Western blotting experiments demonstrated that the disruptant did not produce full-length or truncated forms of RNase III. Thus, as is the case in Streptomyces coelicolor, RNase III is required for antibiotic production in S. antibioticus. No differences in the chemical half-lives of bulk mRNA were observed in a comparison of the S. antibioticus rnc mutant and its parental strain.
authors: Department of Biology, Emory University, Atlanta, Georgia, USA.
authors: Jung-Hoon
authors: JH
authors: Lee
authors: Marcha L
authors: ML
authors: Gatewood
authors: George H
authors: GH
authors: Jones
mesh_terms: D015139:Blotting, Southern; D015153:Blotting, Western; D003470:Culture Media / Q000737:chemistry; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D003609:Dactinomycin / Q000096:biosynthesis*; D055786:Gene Knockout Techniques; D005816:Genetic Complementation Test; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D043244:Ribonuclease III / Q000235:genetics / Q000378:metabolism*; D017422:Sequence Analysis, DNA; D013303:Streptomyces antibioticus / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*
chemical_list: D003470:Culture Media; D004269:DNA, Bacterial; D003609:Dactinomycin; D043244:Ribonuclease III
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