title: Full screening and accurate subtyping of HLA-A*02 alleles through group-specific amplification and mono-allelic sequencing.
abstract: HLA-A*02 is the most prevalent and polymorphic major histocompatibility complex (MHC) allele family in humans. Functional differences have been revealed among subtypes, demanding further subtyping of HLA-A*02 in basic and clinical settings. However, the fast growing polymorphisms render traditional primer- or probe-based typing methods impractical and result in increasing ambiguities in direct sequence-based typing. In this study, we combined group-specific amplification and mono-allelic sequencing to design and validate a simple scheme for the complete screening and accurate subtyping of all 540 reported HLA-A*02 alleles. This scheme could be performed in routine labs to facilitate studies with an interest in HLA-A*02.
authors: Shengli
authors: S
authors: Song
authors: Miaomiao
authors: M
authors: Han
authors: Han
authors: H
authors: Zhang
authors: Yuanxia
authors: Y
authors: Wang
authors: Hong
authors: H
authors: Jiang
mesh_terms: D000483:Alleles; D001483:Base Sequence; D002681:China; D019295:Computational Biology; D004247:DNA / Q000235:genetics; D005091:Exons / Q000235:genetics; D005820:Genetic Testing; D015894:Genome, Human / Q000235:genetics; D015789:HLA-A2 Antigen / Q000235:genetics*; D006238:Haploidy; D006650:Histocompatibility Testing; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D012015:Reference Standards; D015203:Reproducibility of Results; D017422:Sequence Analysis, DNA / Q000379:methods*
chemical_list: C524866:HLA-A*02 antigen; D015789:HLA-A2 Antigen; D004247:DNA
references: Tissue Antigens. 1995 May;45(5):343-7
references: 7652742
references: Tissue Antigens. 1996 Dec;48(6):673-9
references: 9008310
references: J Immunol. 1995 Nov 15;155(10):4749-56
references: 7594476
references: Tissue Antigens. 1998 Mar;51(3):312-4
references: 9550335
references: Cell Mol Immunol. 2006 Dec;3(6):453-8
references: 17257499
references: Tissue Antigens. 1998 Oct;52(4):389-92
references: 9820604
references: J Exp Med. 1995 Dec 1;182(6):1847-56
references: 7500030
references: BMC Genomics. 2011 Jan 18;12:42
references: 21244689
references: Tissue Antigens. 1995 Apr;45(4):223-31
references: 7638857
references: Immunogenetics. 1995;41(1):50
references: 7806276
references: Nucleic Acids Res. 2013 Jan;41(Database issue):D1222-7
references: 23080122
references: Eur J Immunogenet. 1995 Jun;22(3):283-7
references: 8547235
references: Tissue Antigens. 1995 Jan;45(1):1-11
references: 7725305
references: Tissue Antigens. 2012 Jul;80(1):1-11
references: 22651253
references: Methods Mol Biol. 2012;882:9-25
references: 22665226
references: Tissue Antigens. 2000 Jun;55(6):532-47
references: 10902609
references: Tissue Antigens. 1997 Feb;49(2):129-33
references: 9062968
references: Tissue Antigens. 1995 Mar;45(3):177-87
references: 7761977
references: Eur J Immunol. 2011 Jul;41(7):2097-106
references: 21538979
references: Tissue Antigens. 1996 Mar;47(3):237-44
references: 8740775
references: Methods Mol Med. 2007;134:61-9
references: 17666743
references: Tissue Antigens. 1997 Aug;50(2):178-91
references: 9271828
references: Immunol Res. 2012 Sep;53(1-3):182-90
references: 22434516
references: Methods Mol Biol. 2003;210:191-222
references: 12412456