title: Isolation of monomeric photosystem II that retains the subunit PsbS.
abstract: Photosystem II has been purified from a transplastomic strain of Nicotiana tabacum according to two different protocols. Using the procedure described in Piano et al. (Photosynth Res 106:221-226, 2010) it was possible to isolate highly active PSII composed of monomers and dimers but depleted in their PsbS protein content. A "milder" procedure than the protocol reported by Fey et al. (Biochim Biophys Acta 1777:1501-1509, 2008) led to almost exclusively monomeric PSII complexes which in part still bind the PsbS protein. This finding might support a role for PSII monomers in higher plants.
authors: International Institute of Molecular and Cell Biology, Ul. Ks. Trojdena 4, 02109, Warsaw, Poland.
authors: Patrycja
authors: P
authors: Haniewicz
authors: Daniele
authors: D
authors: De Sanctis
authors: Claudia
authors: C
authors: Büchel
authors: Wolfgang P
authors: WP
authors: Schröder
authors: Maria Cecilia
authors: MC
authors: Loi
authors: Thomas
authors: T
authors: Kieselbach
authors: Matthias
authors: M
authors: Bochtler
authors: Dario
authors: D
authors: Piano
mesh_terms: D002734:Chlorophyll / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D045342:Light-Harvesting Protein Complexes / Q000302:isolation & purification* / Q000378:metabolism; D013058:Mass Spectrometry; D010100:Oxygen / Q000378:metabolism*; D010788:Photosynthesis; D045332:Photosystem II Protein Complex / Q000302:isolation & purification* / Q000378:metabolism; D010940:Plant Proteins / Q000378:metabolism; D021122:Protein Subunits; D020524:Thylakoids / Q000378:metabolism; D014026:Nicotiana / Q000378:metabolism / Q000502:physiology*
chemical_list: D045342:Light-Harvesting Protein Complexes; D045332:Photosystem II Protein Complex; D010940:Plant Proteins; D021122:Protein Subunits; D002734:Chlorophyll; D010100:Oxygen
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