title: The expression of 11β-HSDs, GR, and H6PDH in subcutaneous adipose tissue from polycystic ovary syndrome subjects.
abstract: The aim of the work was to investigate the expression of 11β-hydroxysteroid dehydrogenase (11β-HSD) type 1 and 2, hexose-6-phosphate dehydrogenase (H6PDH), and glucocorticoids receptor (GR) mRNA in subcutaneous adipose tissue (SAT) from obese women with or without polycystic ovary syndrome (PCOS), and the association between their expression and the adipocytokines' concentration. Sixteen women with PCOS (group P) and 18 age- and BMI-matched control women (group C) were enrolled for the study. Subcutaneous adipose tissue was collected from the abdomen. The genes' expression was detected by real-time PCR, and the adipocytokines' concentration was measured by ELISA. Peripheral insulin sensitivity was assessed by homeostatic assessment model of insulin resistance (HOMA-IR). β-cell function was assessed by homeostasis model assessment of β-cell function (HOMA-IS). The expression of 11β-HSD1 mRNA was significantly higher in PCOS subjects (p<0.05) than controls; there was no difference for the expression of 11β-HSD2, GR, and H6PDH mRNA between the 2 groups. The stepwise multiple linear regression analysis showed that the mRNA level of 11β-HSD1 was positively correlated to the concentration of the serum tumor necrosis factor-alpha (TNF-α). Expression of 11β-HSD1 mRNA was increased in the SAT from the women with PCOS, which may contribute to the increased local active glucocorticoids (cortisol), and subsequently affects the secretion of the local adipose tissue.
authors: Division of Endocrinology and Metabolism, Department of Internal Medicine, Renji Hospital Affiliated to Shanghai Jiaotong University School of Medicine, Shanghai, P. R. China.
authors: S
authors: S
authors: Li
authors: T
authors: T
authors: Tao
authors: L
authors: L
authors: Wang
authors: X
authors: X
authors: Mao
authors: J
authors: J
authors: Zheng
authors: A
authors: A
authors: Zhao
authors: W
authors: W
authors: Liu
mesh_terms: D043205:11-beta-Hydroxysteroid Dehydrogenase Type 1 / Q000235:genetics / Q000378:metabolism*; D043209:11-beta-Hydroxysteroid Dehydrogenase Type 2 / Q000235:genetics / Q000378:metabolism*; D054392:Adipokines / Q000378:metabolism; D000328:Adult; D000886:Anthropometry; D002237:Carbohydrate Dehydrogenases / Q000235:genetics / Q000378:metabolism*; D016022:Case-Control Studies; D016207:Cytokines / Q000097:blood; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D007328:Insulin / Q000378:metabolism; D011085:Polycystic Ovary Syndrome / Q000097:blood / Q000201:enzymology* / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011965:Receptors, Glucocorticoid / Q000235:genetics / Q000378:metabolism*; D050151:Subcutaneous Fat / Q000201:enzymology*
chemical_list: D054392:Adipokines; D016207:Cytokines; D007328:Insulin; D012333:RNA, Messenger; D011965:Receptors, Glucocorticoid; D002237:Carbohydrate Dehydrogenases; C020552:galactose-6-phosphate dehydrogenase; D043205:11-beta-Hydroxysteroid Dehydrogenase Type 1; D043209:11-beta-Hydroxysteroid Dehydrogenase Type 2; C468386:HSD11B1 protein, human; C468387:HSD11B2 protein, human