title: Deletion of GBG1/AYR1 Alters Cell Wall Biogenesis in Saccharomyces cerevisiae.
abstract: We identified a gene for β-1,3-glucan synthesis (GBG1), a nonessential gene whose disruption alters cell wall synthesis enzyme activities and cell wall composition. This gene was cloned by functional complementation of defects in β-1,3-glucan synthase activity of the the previously isolated Saccharomyces cerevisiae mutant LP0353, which displays a number of cell wall defects at restrictive temperature. Disruption of the GBG1 gene did not affect cell viability or growth rate, but did cause alterations in cell wall synthesis enzyme activities: reduction of β-1,3-glucan synthase and chitin synthase III activities as well as increased chitin synthase I and II activities. GBG1 disruption also showed altered cell wall composition as well as susceptibility toward cell wall inhibitors such as Zymolyase, Calcofluor white, and Nikkomycin Z. These results indicate that GBG1 plays a role in cell wall biogenesis in S. cerevisiae.
authors: Department of Microbiology & Molecular Biology, College of Bioscience and Biotechnology, Chungnam National University, Daejeon 305-764, Korea.
authors: Ki-Woong
authors: KW
authors: Ahn
authors: Sung Woo
authors: SW
authors: Kim
authors: Hyung-Gyoo
authors: HG
authors: Kang
authors: Ki-Hyun
authors: KH
authors: Kim
authors: Yun-Hee
authors: YH
authors: Park
authors: Won-Ja
authors: WJ
authors: Choi
authors: Hee-Moon
authors: HM
authors: Park
keywords: Cell wall composition; Chitin synthase; GBG1/AYR1; Saccharomyces cerevisiae; β-1,3-glucan synthase
references: Mol Microbiol. 2002 Jun;44(5):1167-83
references: 12068804
references: Antimicrob Agents Chemother. 1995 Jan;39(1):200-7
references: 7695307
references: Crit Rev Microbiol. 1987;15(1):7-25
references: 3319422
references: Microbiology (Reading). 2002 Dec;148(Pt 12):4035-4048
references: 12480907
references: Anal Biochem. 1994 Jun;219(2):368-72
references: 8080094
references: J Biol Chem. 2000 Jan 7;275(1):235-40
references: 10617610
references: Biochem J. 1973 Sep;135(1):19-30
references: 4359920
references: Mol Cell Biol. 1995 Oct;15(10):5671-81
references: 7565718
references: Biochim Biophys Acta. 1999 Jun 8;1450(2):145-54
references: 10354506
references: Mol Cell Biol. 1989 Jun;9(6):2695-705
references: 2503710
references: Eukaryot Cell. 2003 Aug;2(4):746-55
references: 12912894
references: Curr Genet. 2009 Aug;55(4):365-80
references: 19466415
references: Mol Biol Cell. 2006 Mar;17(3):1436-50
references: 16407407
references: Antimicrob Agents Chemother. 1994 Dec;38(12):2750-7
references: 7695257
references: Microbiol Sci. 1988 Dec;5(12):370-5
references: 3079180
references: Methods Cell Biol. 1989;31:357-435
references: 2476649
references: J Biol Chem. 1951 Nov;193(1):265-75
references: 14907713
references: Mol Cell Biol. 1997 Apr;17(4):1848-59
references: 9121433
references: Eukaryot Cell. 2007 Feb;6(2):143-56
references: 17158736
references: Proc Natl Acad Sci U S A. 1986 Aug;83(16):5808-12
references: 2942941
references: FEBS Lett. 2000 Jul 28;478(1-2):84-8
references: 10922474
references: Mol Cell Biochem. 2006 Jun;286(1-2):107-14
references: 16496214
references: J Bacteriol. 1983 Jan;153(1):163-8
references: 6336730
references: J Bacteriol. 1997 Dec;179(24):7653-62
references: 9401022
references: Science. 2004 Jun 11;304(5677):1644-7
references: 15192221