title: Nitrogen metabolism of two contrasting poplar species during acclimation to limiting nitrogen availability.
abstract: To investigate N metabolism of two contrasting Populus species in acclimation to low N availability, saplings of slow-growing species (Populus popularis, Pp) and a fast-growing species (Populus alba × Populus glandulosa, Pg) were exposed to 10, 100, or 1000 μM NH4NO3. Despite greater root biomass and fine root surface area in Pp, lower net influxes of NH4(+) and NO3(-) at the root surface were detected in Pp compared to those in Pg, corresponding well to lower NH4(+) and NO3(-) content and total N concentration in Pp roots. Meanwhile, higher stable N isotope composition (δ(15)N) in roots and stronger responsiveness of transcriptional regulation of 18 genes involved in N metabolism were found in roots and leaves of Pp compared to those of Pg. These results indicate that the N metabolism of Pp is more sensitive to decreasing N availability than that of Pg. In both species, low N treatments decreased net influxes of NH4(+) and NO3(-), root NH4(+) and foliar NO3(-) content, root NR activities, total N concentration in roots and leaves, and transcript levels of most ammonium (AMTs) and nitrate (NRTs) transporter genes in leaves and genes involved in N assimilation in roots and leaves. Low N availability increased fine root surface area, foliar starch concentration, δ(15)N in roots and leaves, and transcript abundance of several AMTs (e.g. AMT1;2) and NRTs (e.g. NRT1;2 and NRT2;4B) in roots of both species. These data indicate that poplar species slow down processes of N acquisition and assimilation in acclimation to limiting N supply.
authors: College of Life Sciences and State Key Laboratory of Crop Stress Biology in Arid Areas, Northwest A&F University, Yangling, Shaanxi 712100, PR China.
authors: Jie
authors: J
authors: Luo
authors: Hong
authors: H
authors: Li
authors: Tongxian
authors: T
authors: Liu
authors: Andrea
authors: A
authors: Polle
authors: Changhui
authors: C
authors: Peng
authors: Zhi-Bin
authors: ZB
authors: Luo
mesh_terms: D000064:Acclimatization / Q000187:drug effects*; D064751:Ammonium Compounds / Q000378:metabolism; D002462:Cell Membrane / Q000187:drug effects / Q000201:enzymology; D016000:Cluster Analysis; D003433:Crosses, Genetic; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D008903:Minerals / Q000378:metabolism; D008954:Models, Biological; D009566:Nitrates / Q000378:metabolism; D009573:Nitrites / Q000378:metabolism; D009584:Nitrogen / Q000378:metabolism* / Q000494:pharmacology*; D009587:Nitrogen Isotopes; D010788:Photosynthesis / Q000187:drug effects; D018515:Plant Leaves / Q000187:drug effects / Q000201:enzymology; D018517:Plant Roots / Q000033:anatomy & histology / Q000187:drug effects / Q000201:enzymology / Q000502:physiology; D032107:Populus / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D025341:Principal Component Analysis; D006180:Proton-Translocating ATPases / Q000378:metabolism; D013045:Species Specificity; D014158:Transcription, Genetic / Q000187:drug effects
chemical_list: D064751:Ammonium Compounds; D008903:Minerals; D009566:Nitrates; D009573:Nitrites; D009587:Nitrogen Isotopes; D006180:Proton-Translocating ATPases; D009584:Nitrogen
keywords: Gene expression; glutamate synthase; glutamine synthetase; net flux; nitrate reductase; nitrite reductase; plasma membrane H#x002B;-ATPase; poplar; stable carbon isotope.
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