title: Novel biomarkers of arterial and venous ischemia in microvascular flaps.
abstract: The field of reconstructive microsurgery is experiencing tremendous growth, as evidenced by recent advances in face and hand transplantation, lower limb salvage after trauma, and breast reconstruction. Common to all of these procedures is the creation of a nutrient vascular supply by microsurgical anastomosis between a single artery and vein. Complications related to occluded arterial inflow and obstructed venous outflow are not uncommon, and can result in irreversible tissue injury, necrosis, and flap loss. At times, these complications are challenging to clinically determine. Since early intervention with return to the operating room to re-establish arterial inflow or venous outflow is key to flap salvage, the accurate diagnosis of early stage complications is essential. To date, there are no biochemical markers or serum assays that can predict these complications. In this study, we utilized a rat model of flap ischemia in order to identify the transcriptional signatures of venous congestion and arterial ischemia. We found that the critical ischemia time for the superficial inferior epigastric fasciocutaneus flap was four hours and therefore performed detailed analyses at this time point. Histolgical analysis confirmed significant differences between arterial and venous ischemia. The transcriptome of ischemic, congested, and control flap tissues was deciphered by performing Affymetrix microarray analysis and verified by qRT-PCR. Principal component analysis revealed that arterial ischemia and venous congestion were characterized by distinct transcriptomes. Arterial ischemia and venous congestion was characterized by 408 and 1536>2-fold differentially expressed genes, respectively. qRT-PCR was used to identify five candidate genes Prol1, Muc1, Fcnb, Il1b, and Vcsa1 to serve as biomarkers for flap failure in both arterial ischemia and venous congestion. Our data suggests that Prol1 and Vcsa1 may be specific indicators of venous congestion and allow clinicians to both diagnose and successfully treat microvascular complications before irreversible tissue damage and flap loss occurs.
authors: Division of Plastic & Reconstructive Surgery, Department of Surgery, Keck School of Medicine, University of Southern California, Los Angeles, California, United States of America.
authors: Gerard K
authors: GK
authors: Nguyen
authors: Brian H
authors: BH
authors: Hwang
authors: Yiqiang
authors: Y
authors: Zhang
authors: John F W
authors: JF
authors: Monahan
authors: Gabrielle B
authors: GB
authors: Davis
authors: Yong Suk
authors: YS
authors: Lee
authors: Neli P
authors: NP
authors: Ragina
authors: Charles
authors: C
authors: Wang
authors: Zhao Y
authors: ZY
authors: Zhou
authors: Young Kwon
authors: YK
authors: Hong
authors: Ryan M
authors: RM
authors: Spivak
authors: Alex K
authors: AK
authors: Wong
mesh_terms: D000818:Animals; D001158:Arteries / Q000378:metabolism / Q000473:pathology / Q000601:surgery*; D015415:Biomarkers / Q000378:metabolism*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D063990:Gene Ontology; D006940:Hyperemia / Q000601:surgery; D007511:Ischemia / Q000235:genetics / Q000473:pathology / Q000601:surgery*; D008297:Male; D008866:Microsurgery; D055806:Microvessels / Q000473:pathology / Q000601:surgery*; D020411:Oligonucleotide Array Sequence Analysis; D010641:Phenotype; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction / Q000235:genetics; D013524:Surgical Flaps / Q000098:blood supply* / Q000473:pathology*; D013997:Time Factors; D059467:Transcriptome / Q000235:genetics; D014680:Veins / Q000378:metabolism / Q000473:pathology / Q000601:surgery*
chemical_list: D015415:Biomarkers
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