title: Cultured cell line models of neuronal differentiation: NT2, PC12.
abstract: The lack of a convenient, easily maintained and inexpensive in vitro human neuronal model to study neurodegenerative diseases, prompted us to develop a rapid, 1-h differentiated neuronal cell model based on human NT2 cells and C3 transferase. Here, we describe the rapid differentiation of human neuronal NT2 cells, and the differentiation, transduction and transfection of rat PC12 cells to obtain cells with the morphology of differentiated neurons that can express exogenous genes of interest at high level.
authors: Department of Neuroscience, Temple University School of Medicine, Philadelphia, PA, USA.
authors: Nune
authors: N
authors: Darbinian
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002460:Cell Line; D006801:Humans; D008853:Microscopy; D008938:Mitosis; D009474:Neurons / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D016716:PC12 Cells; D051381:Rats; D013194:Staining and Labeling; D013997:Time Factors; D014161:Transduction, Genetic; D014162:Transfection