title: Metagenomic analysis of the ferret fecal viral flora.
abstract: Ferrets are widely used as a small animal model for a number of viral infections, including influenza A virus and SARS coronavirus. To further analyze the microbiological status of ferrets, their fecal viral flora was studied using a metagenomics approach. Novel viruses from the families Picorna-, Papilloma-, and Anelloviridae as well as known viruses from the families Astro-, Corona-, Parvo-, and Hepeviridae were identified in different ferret cohorts. Ferret kobu- and hepatitis E virus were mainly present in human household ferrets, whereas coronaviruses were found both in household as well as farm ferrets. Our studies illuminate the viral diversity found in ferrets and provide tools to prescreen for newly identified viruses that potentially could influence disease outcome of experimental virus infections in ferrets.
authors: Department of Viroscience, Erasmus Medical Center, Rotterdam, The Netherlands ; Viroclinics Biosciences BV, Rotterdam, The Netherlands.
authors: Saskia L
authors: SL
authors: Smits
authors: V Stalin
authors: VS
authors: Raj
authors: Minoushka D
authors: MD
authors: Oduber
authors: Claudia M E
authors: CM
authors: Schapendonk
authors: Rogier
authors: R
authors: Bodewes
authors: Lisette
authors: L
authors: Provacia
authors: Koert J
authors: KJ
authors: Stittelaar
authors: Albert D M E
authors: AD
authors: Osterhaus
authors: Bart L
authors: BL
authors: Haagmans
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D005243:Feces / Q000821:virology*; D005289:Ferrets / Q000821:virology*; D014644:Genetic Variation; D016679:Genome, Viral / Q000235:genetics; D006801:Humans; D056186:Metagenomics; D008969:Molecular Sequence Data; D010802:Phylogeny; D017386:Sequence Homology, Amino Acid; D014780:Viruses / Q000235:genetics*
references: J Virol. 1998 Oct;72(10):8408-12
references: 9733894
references: J Gen Virol. 2011 Jun;92(Pt 6):1318-1323
references: 21402596
references: Emerg Infect Dis. 2005 Dec;11(12):1842-7
references: 16485468
references: Ann Neurol. 2008 Sep;64(3):266-73
references: 18825694
references: Lancet Infect Dis. 2008 Nov;8(11):698-709
references: 18992406
references: Nature. 2003 Oct 30;425(6961):915
references: 14586458
references: PLoS Pathog. 2013;9(5):e1003343
references: 23717200
references: J Virol. 2002 May;76(9):4420-9
references: 11932409
references: J Clin Microbiol. 2010 May;48(5):1787-94
references: 20335418
references: J Virol. 2012 Apr;86(8):4620-30
references: 22345464
references: Nature. 2009 Jun 25;459(7250):1122-5
references: 19516283
references: Proc Natl Acad Sci U S A. 2005 Sep 6;102(36):12891-6
references: 16118271
references: PLoS One. 2011;6(6):e20579
references: 21674005
references: Bioinformatics. 2007 Nov 1;23(21):2947-8
references: 17846036
references: Philos Trans R Soc Lond B Biol Sci. 2001 Jul 29;356(1411):983-9
references: 11516376
references: Curr Opin Virol. 2012 Feb;2(1):63-77
references: 22440968
references: PLoS Pathog. 2009 Oct;5(10):e1000642
references: 19888339
references: J Virol. 2012 Nov;86(22):12262-70
references: 22951832
references: J Virol. 2010 Jul;84(14):6955-65
references: 20463061
references: Virology. 2007 Jun 5;362(2):404-10
references: 17289104
references: Science. 2005 Sep 9;309(5741):1680-1
references: 16150997
references: Rev Med Virol. 2011 Jan;21(1):32-41
references: 21294214
references: Zoonoses Public Health. 2013 Mar;60(2):117-24
references: 22697485
references: Virol J. 2010 Nov 12;7:313
references: 21073719
references: Arch Virol. 2009;154(1):101-8
references: 19096904
references: Pediatr Infect Dis J. 2009 Jun;28(6):545-7
references: 19483524
references: J Gen Virol. 2011 Nov;92(Pt 11):2534-2541
references: 21775584
references: Vet Microbiol. 2010 Jan 27;140(3-4):256-65
references: 19361937
references: J Am Vet Med Assoc. 1989 Oct 1;195(7):990-4
references: 2676931
references: Virology. 2010 May 25;401(1):70-9
references: 20206957
references: Virology. 2004 Jun 20;324(1):17-27
references: 15183049
references: J Virol. 1999 Jan;73(1):152-60
references: 9847317
references: J Virol. 2011 Oct;85(19):9909-17
references: 21795334
references: Pediatr Infect Dis J. 1992 Jun;11(6):479-87
references: 1608686
references: Emerg Infect Dis. 2010 May;16(5):869-70
references: 20409388
references: Nature. 1989 Jun 1;339(6223):389-92
references: 2786147
references: Proc Natl Acad Sci U S A. 2001 Sep 25;98(20):11609-14
references: 11562506
references: J Clin Virol. 2009 May;45(1):1-9
references: 19372062
references: J Virol. 2012 Feb;86(4):2360-5
references: 22171250
references: Emerg Infect Dis. 2011 Aug;17(8):1570-1
references: 21801658
references: Trends Ecol Evol. 2005 May;20(5):238-44
references: 16701375
references: Rev Infect Dis. 1988 Jan-Feb;10(1):56-75
references: 3281223
references: mBio. 2012 Feb 24;3(2):e00045-12
references: 22366820
references: J Parasitol. 2005 Feb;91(1):179-82
references: 15856896
references: J Virol. 2011 Nov;85(22):11697-708
references: 21900163
references: Emerg Infect Dis. 2011 Oct;17(10):1876-8
references: 22000359
references: Comp Med. 2003 Dec;53(6):579-601
references: 14727806
references: Emerg Infect Dis. 2012 Aug;18(8):1369-70
references: 22840220
references: J Virol. 2011 Nov;85(21):11520-5
references: 21880761
references: Rev Med Virol. 2010 Nov;20(6):392-407
references: 20925048
references: Philos Trans R Soc Lond B Biol Sci. 2001 Jun 29;356(1410):791-3
references: 11405921
references: J Virol. 2006 Aug;80(15):7481-90
references: 16840328
references: Genome Res. 2011 Sep;21(9):1552-60
references: 21690186
references: J Clin Microbiol. 2008 Oct;46(10):3446-53
references: 18753351
references: Mol Biol Evol. 2007 Aug;24(8):1596-9
references: 17488738
references: Virus Genes. 2012 Dec;45(3):518-25
references: 22899339
references: Emerg Infect Dis. 2011 Dec;17(12):2328-30
references: 22172405
references: Nature. 1999 Feb 4;397(6718):436-41
references: 9989410
references: J Gen Virol. 2010 Nov;91(Pt 11):2719-22
references: 20668117
references: Genome Res. 2007 Mar;17(3):377-86
references: 17255551
references: Nucleic Acids Res. 1997 Sep 1;25(17):3389-402
references: 9254694
references: PLoS One. 2011 Apr 29;6(4):e19054
references: 21559501
references: Microbiol Mol Biol Rev. 2010 Sep;74(3):363-77
references: 20805403
references: PLoS Pathog. 2011 Sep;7(9):e1002218
references: 21909269
references: J Virol. 2002 Sep;76(17):8920-30
references: 12163611
references: Hepatology. 2011 Dec;54(6):2218-26
references: 21932388
references: Emerg Infect Dis. 2005 Jan;11(1):103-5
references: 15705330
references: Curr Top Microbiol Immunol. 2009;331:21-33
references: 19230555
references: Proc Natl Acad Sci U S A. 2005 Feb 15;102(7):2430-5
references: 15695582
references: J Gen Virol. 2003 Nov;84(Pt 11):3069-3077
references: 14573811
references: J Virol. 2010 Feb;84(4):1674-82
references: 20007276
references: J Virol. 2009 Mar;83(6):2500-9
references: 19116258