title: Effect of dietary supplemented andrographolide on growth, non-specific immune parameters and resistance against Aeromonas hydrophila in Labeo rohita (Hamilton).
abstract: The present study evaluated the effect of dietary andrographolide (EC 50%) on growth, non-specific immune parameters and disease resistance against Aeromonas hydrophila infection in Indian major carp, Labeo rohita fingerlings. Fishes were fed with formulated diet containing andrographolide as T0 (0.00%), T1 (0.05%), T2 (0.10%), T3 (0.20%), T4 (0.40%) and T5 (0.80%) for 42 days. Fishes were challenged with A. hydrophila 42 days post feeding and relative percentage survival (RPS) was recorded over 14 days post challenge. Blood and serum samples were collected for nonspecific immune parameters on 14, 28 and 42 days of feeding and growth performance was evaluated at the end of experiment. The results revealed that fishes fed with andrographolide showed significant (p < 0.05) increase in NBT levels, myeloperoxidase activity, phagocytic activity, serum lysozyme activity, and serum antiprotease activity when compared to the control group. The weight gain, specific growth rate, feed conversion ratio and protein efficiency ratio of fishes fed with andrographolide were found to be significantly (p < 0.05) differed compared with control. Dietary andrographolide at the level of 0.10% showed significantly (P < 0.05) higher RPS (74.06%) against A. hydrophila infection than control. The results revealed that andrographolide supplemented diet has a stimulatory effect on non-specific immune parameters along with improved growth performance and increased disease resistance against A. hydrophila infection in L. rohita fingerlings.
authors: Central Institute of Fisheries Education, Panch Marg, Off Yari Road, Versova, Mumbai 400 061, India.
authors: Kusunur Ahamed
authors: KA
authors: Basha
authors: Ram Prakash
authors: RP
authors: Raman
authors: Kurcheti Pani
authors: KP
authors: Prasad
authors: Kundan
authors: K
authors: Kumar
authors: Ezhil
authors: E
authors: Nilavan
authors: Saurav
authors: S
authors: Kumar
mesh_terms: D016980:Aeromonas hydrophila; D000704:Analysis of Variance; D000818:Animals; D017756:Aquaculture / Q000379:methods; D003530:Cyprinidae / Q000254:growth & development / Q000276:immunology*; D019587:Dietary Supplements; D060467:Disease Resistance / Q000187:drug effects*; D004224:Diterpenes / Q000494:pharmacology* / Q000627:therapeutic use; D004305:Dose-Response Relationship, Drug; D005393:Fish Diseases / Q000276:immunology* / Q000517:prevention & control*; D016905:Gram-Negative Bacterial Infections / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary*; D009113:Muramidase / Q000097:blood; D009580:Nitroblue Tetrazolium; D009195:Peroxidase / Q000097:blood; D010587:Phagocytosis / Q000187:drug effects / Q000502:physiology; D011480:Protease Inhibitors / Q000097:blood
chemical_list: D004224:Diterpenes; D011480:Protease Inhibitors; D009580:Nitroblue Tetrazolium; C030419:andrographolide; D009195:Peroxidase; D009113:Muramidase
keywords: Aeromonas hydrophila; Andrographolide; Immune system; Immunostimulant; Non-specific