title: In vitro osteogenic/dentinogenic potential of an experimental calcium aluminosilicate cement.
abstract: BACKGROUND
  Calcium aluminosilicate cements are fast-setting, acid-resistant, bioactive cements that may be used as root-repair materials. This study examined the osteogenic/dentinogenic potential of an experimental calcium aluminosilicate cement (Quick-Set) by using a murine odontoblast-like cell model.
  
  
  METHODS
  Quick-Set and white ProRoot MTA (WMTA) were mixed with the proprietary gel or deionized water, allowed to set completely in 100% relative humidity, and aged in complete growth medium for 2 weeks until rendered non-cytotoxic. Similarly aged Teflon disks were used as negative control. The MDPC-23 cell line was used for evaluating changes in mRNA expressions of genes associated with osteogenic/dentinogenic differentiation and mineralization (quantitative reverse transcription polymerase chain reaction), alkaline phosphatase enzyme production, and extracellular matrix mineralization (alizarin red S staining).
  
  
  RESULTS
  After MDPC-23 cells were incubated with the materials in osteogenic differentiation medium for 1 week, both cements showed up-regulation in ALP and DSPP expression. Fold increases in these 2 genes were not significantly different between Quick-Set and WMTA. Both cements showed no statistically significant up-regulation/down-regulation in RUNX2, OCN, BSP, and DMP1 gene expression compared with Teflon. Alkaline phosphatase activity of cells cultured on Quick-Set and WMTA were not significantly different at 1 week or 2 weeks but were significantly higher (P < .05) than Teflon in both weeks. Both cements showed significantly higher calcium deposition compared with Teflon after 3 weeks of incubation in mineralizing medium (P < .001). Differences between Quick-Set and WMTA were not statistically significant.
  
  
  CONCLUSIONS
  The experimental calcium aluminosilicate cement exhibits similar osteogenic/dentinogenic properties to WMTA and may be a potential substitute for commercially available tricalcium silicate cements.
authors: Department of Dental and Biomedical Material Sciences, Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki, Japan.
authors: Ashraf A
authors: AA
authors: Eid
authors: Li-na
authors: LN
authors: Niu
authors: Carolyn M
authors: CM
authors: Primus
authors: Lynne A
authors: LA
authors: Opperman
authors: David H
authors: DH
authors: Pashley
authors: Ikuya
authors: I
authors: Watanabe
authors: Franklin R
authors: FR
authors: Tay
mesh_terms: D000469:Alkaline Phosphatase / Q000187:drug effects; D017607:Aluminum Compounds / Q000494:pharmacology; D000538:Aluminum Silicates / Q000494:pharmacology*; D000818:Animals; D002113:Calcification, Physiologic / Q000187:drug effects; D017610:Calcium Compounds / Q000494:pharmacology*; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D050659:Core Binding Factor Alpha 1 Subunit / Q000187:drug effects; D003470:Culture Media; D003738:Dental Cements / Q000494:pharmacology*; D003810:Dentinogenesis / Q000187:drug effects*; D004338:Drug Combinations; D005109:Extracellular Matrix / Q000187:drug effects; D016326:Extracellular Matrix Proteins / Q000187:drug effects; D006813:Humidity; D058574:Integrin-Binding Sialoprotein / Q000187:drug effects; D008422:Materials Testing; D051379:Mice; D009804:Odontoblasts / Q000187:drug effects*; D015675:Osteocalcin / Q000187:drug effects; D010012:Osteogenesis / Q000187:drug effects*; D010087:Oxides / Q000494:pharmacology; D010750:Phosphoproteins / Q000187:drug effects; D012387:Root Canal Filling Materials / Q000494:pharmacology; D012795:Sialoglycoproteins / Q000187:drug effects; D017640:Silicates / Q000494:pharmacology; D013997:Time Factors; D000099311:Dentin Sialophosphoprotein
chemical_list: D000469:Alkaline Phosphatase; D017607:Aluminum Compounds; D000538:Aluminum Silicates; D017610:Calcium Compounds; D050659:Core Binding Factor Alpha 1 Subunit; D003470:Culture Media; D003738:Dental Cements; D004338:Drug Combinations; D016326:Extracellular Matrix Proteins; D058574:Integrin-Binding Sialoprotein; D015675:Osteocalcin; D010087:Oxides; D010750:Phosphoproteins; D012387:Root Canal Filling Materials; D012795:Sialoglycoproteins; D017640:Silicates; D000099311:Dentin Sialophosphoprotein; C513308:Dmp1 protein, mouse; C546872:Ibsp protein, mouse; C000596909:Quick-Set; C493716:Runx2 protein, mouse; C086631:mineral trioxide aggregate
keywords: Alizarin red S staining; MDPC-23 cells; alkaline phosphatase; calcium aluminosilicate cement; quantitative reverse transcription polymerase chain reaction
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