title: Remodeling of tissue-engineered bone structures in vivo.
abstract: Implant design for bone regeneration is expected to be optimized when implant structures resemble the anatomical situation of the defect site. We tested the validity of this hypothesis by exploring the feasibility of generating different in vitro engineered bone-like structures originating from porous silk fibroin scaffolds decorated with RGD sequences (SF-RGD), seeded with human mesenchymal stem cells (hMSC). Scaffolds with small (106-212 μm), medium (212-300 μm), and large pore diameter ranges (300-425 μm) were seeded with hMSC and subsequently differentiated in vitro into bone-like tissue resembling initial scaffold geometries and featuring bone-like structures. Eight weeks after implantation into calvarial defects in mice, the in vitro engineered bone-like tissues had remodeled into bone featuring different proportions of woven/lamellar bone bridging the defects. Regardless of pore diameter, all implants integrated well, vascularization was advanced, and bone marrow ingrowth had started. Ultimately, in this defect model, the geometry of the in vitro generated tissue-engineered bone structure, trabecular- or plate-like, had no significant impact on the healing of the defect, owing to an efficient remodeling of its structure after implantation.
authors: Institute of Pharmaceutical Sciences, ETH Zurich, Switzerland. sahofmann@ethz.ch
authors: Sandra
authors: S
authors: Hofmann
authors: Monika
authors: M
authors: Hilbe
authors: Robert J
authors: RJ
authors: Fajardo
authors: Henri
authors: H
authors: Hagenmüller
authors: Katja
authors: K
authors: Nuss
authors: Margarete
authors: M
authors: Arras
authors: Ralph
authors: R
authors: Müller
authors: Brigitte
authors: B
authors: von Rechenberg
authors: David L
authors: DL
authors: Kaplan
authors: Hans P
authors: HP
authors: Merkle
authors: Lorenz
authors: L
authors: Meinel
mesh_terms: D000818:Animals; D001861:Bone Regeneration; D016723:Bone Remodeling; D002448:Cell Adhesion; D049109:Cell Proliferation; D002478:Cells, Cultured; D005240:Feasibility Studies; D005349:Fibroins / Q000009:adverse effects / Q000737:chemistry / Q000378:metabolism / Q000627:therapeutic use; D005549:Foreign-Body Reaction / Q000517:prevention & control; D048091:Guided Tissue Regeneration; D006801:Humans; D008422:Materials Testing; D045164:Mesenchymal Stem Cell Transplantation / Q000009:adverse effects; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism; D051379:Mice; D008819:Mice, Nude; D009842:Oligopeptides / Q000737:chemistry / Q000378:metabolism; D016062:Porosity; D011897:Random Allocation; D012886:Skull / Q000166:cytology / Q000293:injuries / Q000502:physiology / Q000601:surgery*; D013047:Specific Pathogen-Free Organisms; D054457:Tissue Scaffolds / Q000009:adverse effects / Q000737:chemistry; D014945:Wound Healing
chemical_list: D009842:Oligopeptides; C482288:fibroin, silkworm; C047981:arginyl-glycyl-aspartic acid; D005349:Fibroins
keywords: Bone; In vivo; Remodeling; Silk fibroin; Stem cell; Tissue engineering
references: Biomaterials. 2003 Feb;24(3):401-16
references: 12423595
references: Expert Opin Biol Ther. 2003 Jun;3(3):409-23
references: 12783610
references: J Biotechnol. 2005 Nov 21;120(3):327-39
references: 16150508
references: Acta Biomater. 2008 Nov;4(6):1904-15
references: 18571999
references: Phys Med Biol. 1994 Jan;39(1):145-64
references: 7651993
references: J Orthop Res. 1991 Sep;9(5):641-50
references: 1870029
references: Biomaterials. 2006 Jun;27(18):3413-31
references: 16504284
references: Biomaterials. 2005 Jun;26(17):3527-36
references: 15621243
references: Stem Cells. 2003;21(5):610-9
references: 12968115
references: Eur Cell Mater. 2004 Sep 13;8:12-9; discussion 20
references: 15494930
references: J Bone Joint Surg Am. 2004 Jul;86(7):1541-58
references: 15252108
references: Bone. 2006 Oct;39(4):922-31
references: 16757219
references: J Tissue Eng Regen Med. 2012 Feb;6(2):155-62
references: 21446054
references: Biomaterials. 2001 Oct;22(19):2581-93
references: 11519777
references: Acta Biomater. 2011 Feb;7(2):478-84
references: 20709195
references: Macromol Biosci. 2004 Aug 9;4(8):743-65
references: 15468269
references: Biomaterials. 2006 Oct;27(28):4993-5002
references: 16765437
references: Biomaterials. 2005 Sep;26(27):5474-91
references: 15860204
references: Tissue Eng. 2003;9 Suppl 1:S127-39
references: 14511476
references: Lancet. 1999 Jul;354 Suppl 1:SI32-4
references: 10437854
references: Nat Biotechnol. 2005 Jan;23(1):47-55
references: 15637621
references: J Tissue Eng Regen Med. 2008 Jun;2(4):169-83
references: 18493906
references: J Biomed Mater Res. 2001 Jan;54(1):139-48
references: 11077413
references: J Tissue Eng Regen Med. 2008 Mar-Apr;2(2-3):71-80
references: 18383453
references: Biomaterials. 2006 Jul;27(21):3964-72
references: 16584771
references: Cells Tissues Organs. 2001;169(3):302-8
references: 11455127
references: Bone. 2007 Aug;41(2):247-55
references: 17553763
references: Tissue Eng. 2006 Dec;12(12):3417-29
references: 17518678
references: Acta Biomater. 2012 Feb;8(2):502-10
references: 22100348
references: J Bone Miner Res. 1999 Jul;14(7):1167-74
references: 10404017
references: Ann Biomed Eng. 2004 Jan;32(1):112-22
references: 14964727
references: Connect Tissue Res. 2002;43(2-3):529-34
references: 12489210
references: Proc Natl Acad Sci U S A. 2010 Feb 23;107(8):3311-6
references: 20133604
references: Bone. 2005 Nov;37(5):688-98
references: 16140599
references: J Bone Joint Surg Am. 2003 Sep;85(9):1739-48
references: 12954833
references: Tissue Eng Part A. 2008 Aug;14(8):1331-40
references: 18601588
references: J Biomed Mater Res B Appl Biomater. 2011 Oct;99(1):89-101
references: 21695778
references: Trends Mol Med. 2009 Sep;15(9):417-29
references: 19740701
references: J Cell Mol Med. 2005 Jan-Mar;9(1):72-84
references: 15784166
references: Biomaterials. 2004 Mar;25(6):1039-47
references: 14615169
references: Science. 1993 May 14;260(5110):920-6
references: 8493529
references: Biomaterials. 2007 Feb;28(6):1152-62
references: 17092555
references: Biomaterials. 2005 Jun;26(17):3385-93
references: 15621227
references: Clin Orthop Relat Res. 1986 Dec;(213):34-40
references: 3780104
references: Biomaterials. 2005 Jan;26(2):147-55
references: 15207461
references: Biomacromolecules. 2004 May-Jun;5(3):718-26
references: 15132652
references: Tissue Eng. 2003;9 Suppl 1:S113-26
references: 14511475
references: Biomaterials. 2006 Dec;27(36):6064-82
references: 16890988
references: Biomaterials. 2003 Aug;24(18):3079-85
references: 12895580
references: Calcif Tissue Int. 1996 Jan;58(1):24-9
references: 8825235
references: Biomaterials. 2006 Oct;27(30):5186-98
references: 16790273
references: J Cell Mol Med. 2006 Jan-Mar;10(1):7-19
references: 16563218
references: Biotechnol Bioeng. 2004 Nov 5;88(3):379-91
references: 15486944
references: Prog Polym Sci. 2007;32(8-9):991-1007
references: 19543442
references: Biomaterials. 2007 Jan;28(2):134-46
references: 17011028
references: J Biomed Mater Res A. 2004 Oct 1;71(1):25-34
references: 15316936
references: J Biomed Mater Res. 1998 Feb;39(2):190-9
references: 9457547
references: Ann Biomed Eng. 2004 Jan;32(1):148-59
references: 14964730
references: Biomaterials. 2006 Jun;27(17):3230-7
references: 16488007
references: Methods Mol Biol. 2012;868:27-43
references: 22692602