title: H3K27 demethylase, JMJD3, regulates fragmentation of spermatogonial cysts.
abstract: The spermatogonial stem cell (SSC) compartment is maintained by self-renewal of stem cells as well as fragmentation of differentiating spermatogonia through abscission of intercellular bridges in a random and stochastic manner. The molecular mechanisms that regulate this reversible developmental lineage remain to be elucidated. Here, we show that histone H3K27 demethylase, JMJD3 (KDM6B), regulates the fragmentation of spermatogonial cysts. Down-regulation of Jmjd3 in SSCs promotes an increase in undifferentiated spermatogonia but does not affect their differentiation. Germ cell-specific Jmjd3 null male mice have larger testes and sire offspring for a longer period compared to controls, likely secondary to increased and prolonged maintenance of the spermatogonial compartment. Moreover, JMJD3 deficiency induces frequent fragmentation of spermatogonial cysts by abscission of intercellular bridges. These results suggest that JMJD3 controls the spermatogonial compartment through the regulation of fragmentation of spermatogonial cysts and this mechanism may be involved in maintenance of diverse stem cell niches.
authors: Departments of Pathology & Immunology, Baylor College of Medicine, Houston, Texas, United States of America. iwamori@anim.med.kyushu-u.ac.jp
authors: Naoki
authors: N
authors: Iwamori
authors: Tokuko
authors: T
authors: Iwamori
authors: Martin M
authors: MM
authors: Matzuk
mesh_terms: D000818:Animals; D054337:Cell Dedifferentiation; D002454:Cell Differentiation / Q000235:genetics; D015870:Gene Expression; D055785:Gene Knockdown Techniques; D006657:Histones / Q000378:metabolism; D056484:Jumonji Domain-Containing Histone Demethylases / Q000235:genetics* / Q000378:metabolism*; D008297:Male; D008745:Methylation; D051379:Mice; D010641:Phenotype; D021381:Protein Transport; D013091:Spermatogenesis / Q000502:physiology; D013093:Spermatogonia / Q000166:cytology / Q000502:physiology*; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism; D013737:Testis / Q000166:cytology / Q000378:metabolism
chemical_list: D006657:Histones; D056484:Jumonji Domain-Containing Histone Demethylases; C523761:Kdm6b protein, mouse
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