title: DNA vaccine that targets hemagglutinin to MHC class II molecules rapidly induces antibody-mediated protection against influenza.
abstract: New influenza A viruses with pandemic potential periodically emerge due to viral genomic reassortment. In the face of pandemic threats, production of conventional egg-based vaccines is time consuming and of limited capacity. We have developed in this study a novel DNA vaccine in which viral hemagglutinin (HA) is bivalently targeted to MHC class II (MHC II) molecules on APCs. Following DNA vaccination, transfected cells secreted vaccine proteins that bound MHC II on APCs and initiated adaptive immune responses. A single DNA immunization induced within 8 d protective levels of strain-specific Abs and also cross-reactive T cells. During the Mexican flu pandemic, a targeted DNA vaccine (HA from A/California/07/2009) was generated within 3 wk after the HA sequences were published online. These results suggest that MHC II-targeted DNA vaccines could play a role in situations of pandemic threats. The vaccine principle should be extendable to other infectious diseases.
authors: Centre for Immune Regulation, Institute of Immunology, University of Oslo and Oslo University Hospital, Oslo 0027, Norway. gunnveig.grodeland@medisin.uio.no
authors: Gunnveig
authors: G
authors: Grodeland
authors: Siri
authors: S
authors: Mjaaland
authors: Kenneth H
authors: KH
authors: Roux
authors: Agnete Brunsvik
authors: AB
authors: Fredriksen
authors: Bjarne
authors: B
authors: Bogen
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D015153:Blotting, Western; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry; D006388:Hemagglutinins / Q000276:immunology*; D000949:Histocompatibility Antigens Class II / Q000276:immunology*; D053118:Influenza A Virus, H1N1 Subtype / Q000235:genetics / Q000276:immunology; D007252:Influenza Vaccines / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D009976:Orthomyxoviridae Infections / Q000276:immunology / Q000517:prevention & control; D058873:Pandemics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013030:Spain / Q000453:epidemiology; D014162:Transfection; D019444:Vaccines, DNA / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D006388:Hemagglutinins; D000949:Histocompatibility Antigens Class II; D007252:Influenza Vaccines; D019444:Vaccines, DNA
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