title: 8-hydroxy-2'-deoxyguanosine in colorectal adenocarcinoma--is it a result of oxidative stress?
abstract: BACKGROUND
  8-hydroxy-2'-deoxyguanosine (8-OHdG) is one of the most abundant oxidatively modified lesions in DNA and is a marker of the oxidative stress. 8-OHdG is a mutagenic lesion and it can mispair with adenine, causing G:C→T: A transversion. Our task was to determine the 8-OHdG level in patients with colorectal adenocarcinoma directly in tumor tissues and corresponding normal mucosa.
  
  
  METHODS
  Samples of tumor tissues and corresponding normal mucosa of 47 patients undergoing surgery for colorectal cancer were analyzed. DNA was isolated from both tumor and normal tissues. Then, DNA was hydrolyzed to nucleotides using nuclease P1 and alkaline phosphatase. The 8-OHdG and 2'-dG (2'-deoxyguanosine) were determined in hydrolysates by high-performance liquid chromatography (HPLC) with electrochemical (EC) and UV detector.
  
  
  RESULTS
  The levels of 8-OHdG in colorectal adenocarcinoma tissues were higher than in corresponding normal mucosa. No significant differences were shown in 8-OHdG levels in the cancerous and cancer-free tissues between age and sex and stages A/B and C/D of Duke's classification.
  
  
  CONCLUSIONS
  8-OHdG reflects the local oxidative stress in colon adenocarcinoma tissue together with ageing processes, but not the intensity of tumorigenesis itself. Because of many factors that could influence the oxidative modification of DNA bases, its role as a diagnostic and/or prognostic factor in colon adenocarcinoma seems to be limited.
authors: Chair and Department of General Biology, Medical University of Silesia, Zabrze, Poland. aplachetka@sum.edu.pl
authors: Anna
authors: A
authors: Płachetka
authors: Brygida
authors: B
authors: Adamek
authors: Joanna K
authors: JK
authors: Strzelczyk
authors: Łukasz
authors: Ł
authors: Krakowczyk
authors: Paweł
authors: P
authors: Migula
authors: Przemysław
authors: P
authors: Nowak
authors: Andrzej
authors: A
authors: Wiczkowski
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000230:Adenocarcinoma / Q000378:metabolism*; D000328:Adult; D002851:Chromatography, High Pressure Liquid; D015179:Colorectal Neoplasms / Q000378:metabolism*; D004247:DNA / Q000032:analysis*; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000032:analysis; D005260:Female; D006801:Humans; D008297:Male; D018384:Oxidative Stress / Q000502:physiology*; D011044:Poland; D018709:Statistics, Nonparametric
chemical_list: D000080242:8-Hydroxy-2'-Deoxyguanosine; D004247:DNA; D003849:Deoxyguanosine
references: FASEB J. 1998 Oct;12(13):1397-400
references: 9761783
references: PLoS One. 2011 Apr 14;6(4):e18532
references: 21533223
references: Mutat Res. 1999 Mar 8;424(1-2):9-21
references: 10064846
references: Cancer Epidemiol Biomarkers Prev. 1998 Feb;7(2):141-6
references: 9488589
references: Free Radic Biol Med. 2011 Oct 1;51(7):1320-8
references: 21820046
references: Dig Dis Sci. 2007 Feb;52(2):526-30
references: 17195121
references: Biol Chem. 2006 Apr;387(4):373-9
references: 16606334
references: J Cancer Res Clin Oncol. 2004 May;130(5):253-8
references: 14963700
references: Mol Carcinog. 1997 Apr;18(4):232-43
references: 9142218
references: Biol Chem. 2006 Apr;387(4):365-72
references: 16606333
references: Autophagy. 2007 Jan-Feb;3(1):26-7
references: 16963838
references: Neoplasma. 2007;54(2):131-6
references: 17319786
references: Br J Cancer. 1958 Sep;12(3):309-20
references: 13596482
references: Methods Enzymol. 1990;186:521-30
references: 2233317
references: Res Rep Health Eff Inst. 2012 Sep;(166):163-84
references: 23156842
references: Lab Invest. 2011 Dec;91(12):1777-86
references: 21968813
references: FASEB J. 2005 Jan;19(1):82-4
references: 15533950
references: S Afr Med J. 2008 Aug;98(8):614-7
references: 18928040
references: World J Gastroenterol. 2012 Jan 28;18(4):302-8
references: 22294836
references: Oxid Med Cell Longev. 2011;2011:760978
references: 21969876
references: Br J Cancer. 2010 Mar 16;102(6):1018-23
references: 20179711
references: Crit Rev Clin Lab Sci. 2002 Sep;39(4-5):331-457
references: 12385502
references: Eur J Cancer. 2001 Feb;37(3):340-6
references: 11239755
references: Horm Cancer. 2013 Apr;4(2):61-9
references: 23307762
references: Clin Chem. 2006 Apr;52(4):601-23
references: 16484333
references: Free Radic Res. 2000 May;32(5):381-97
references: 10766407
references: APMIS. 2007 Feb;115(2):81-103
references: 17295675
references: Mutat Res. 2008 Jul 3;642(1-2):37-42
references: 18495177
references: Mutat Res. 1999 Jan 2;438(1):23-7
references: 9858672
references: Antioxid Redox Signal. 2006 May-Jun;8(5-6):1021-31
references: 16771692
references: Clin Cancer Res. 2000 Apr;6(4):1394-400
references: 10778969
references: J Clin Biochem Nutr. 2010 Jul;47(1):59-63
references: 20664732
references: Free Radic Biol Med. 2007 Sep 1;43(5):853-9
references: 17664149
references: Biomed Environ Sci. 2008 Aug;21(4):286-9
references: 18837290
references: J Environ Sci Health C Environ Carcinog Ecotoxicol Rev. 2009 Apr;27(2):120-39
references: 19412858
references: Nature. 1991 Jan 31;349(6308):431-4
references: 1992344
references: Int Arch Occup Environ Health. 2006 Oct;80(1):1-15
references: 16685565
references: J Gerontol A Biol Sci Med Sci. 2001 Nov;56(11):B483-5
references: 11682569
references: J Natl Cancer Inst. 2003 Sep 3;95(17):1263-5
references: 12953074
references: Carcinogenesis. 1992 Dec;13(12):2241-7
references: 1473230
references: Crit Rev Food Sci Nutr. 2007;47(1):27-50
references: 17364694
references: Hepatogastroenterology. 2003 Jan-Feb;50(49):126-31
references: 12630007
references: Environ Health Perspect. 1993 Mar;99:261-3
references: 8319639
references: Int J Cancer. 2002 Oct 1;101(4):395-7
references: 12209966
references: Clin Chem Lab Med. 2011 Sep 13;49(12):2007-12
references: 21913795
references: Mutat Res. 2011 Jun 3;711(1-2):193-201
references: 21216256
references: Free Radic Biol Med. 1999 Aug;27(3-4):401-10
references: 10468215