title: In vivo imaging and histochemistry are combined in the cryosection labelling and intravital microscopy technique.
abstract: Intravital microscopy has been used extensively to study dynamic processes in the context of a living animal; however, only a limited number of fluorescent probes and mouse models are available. By contrast, many dyes and antibodies exist for the immuno-labelling of fixed tissue. Here we report a method that combines the advantages of histochemistry and in vivo imaging by correlating cryosection labelling with corresponding intravital microscopy images (CLIM). Using CLIM, we find that the presence of CD3(+) T cells correlates with mammary tumour cell migration. When CD4(+) and CD8(+) T-cell subsets are depleted, reduced tumour cell migration is observed. From these data we conclude that CLIM is a powerful tool to correlate intravital microscopy data with cryosection labelling data.
authors: Hubrecht Institute-Royal Dutch Academy of Science and University Medical Center Utrecht, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands.
authors: Laila
authors: L
authors: Ritsma
authors: Nienke
authors: N
authors: Vrisekoop
authors: Jacco
authors: J
authors: van Rheenen
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D002465:Cell Movement; D016616:Cryoultramicrotomy / Q000379:methods*; D005615:Freezing; D021621:Imaging, Three-Dimensional; D007150:Immunohistochemistry / Q000379:methods*; D007834:Lasers; D051379:Mice; D008807:Mice, Inbred BALB C; D008853:Microscopy / Q000379:methods*; D009369:Neoplasms / Q000276:immunology / Q000473:pathology; D017785:Photons; D044967:Serum / Q000166:cytology; D013194:Staining and Labeling; D013601:T-Lymphocytes / Q000473:pathology