title: Identification of protein biomarkers in human serum using iTRAQ and shotgun mass spectrometry.
abstract: Blood serum is one of the easiest accessible sources of biomarkers and its proteome presents a significant parcel of immune system proteins. These proteins can provide not only biological explanation but also diagnostic and drug response answers independently of the type of disease or condition in question. Shotgun mass spectrometry has profoundly contributed to proteome analysis and is presently considered as an indispensible tool in the field of biomarker discovery. In addition, the multiplexing potential of isotopic labeling techniques such as iTRAQ can increase statistical relevance and accuracy of proteomic data through the simultaneous analysis of different biological samples. Here, we describe a complete protocol using iTRAQ in a shotgun proteomics workflow along with data analysis steps, customized for the challenges associated with the serum proteome.
authors: Department of Chemical Engineering and Biotechnology, Cambridge Centre for Neuropsychiatric Research, University of Cambridge, Cambridge, UK.
authors: Theodoros A
authors: TA
authors: Koutroukides
authors: Julian A J
authors: JA
authors: Jaros
authors: Bob
authors: B
authors: Amess
authors: Daniel
authors: D
authors: Martins-de-Souza
authors: Paul C
authors: PC
authors: Guest
authors: Hassan
authors: H
authors: Rahmoune
authors: Yishai
authors: Y
authors: Levin
authors: Mike
authors: M
authors: Deery
authors: Philip D
authors: PD
authors: Charles
authors: Svenja
authors: S
authors: Hester
authors: Arnoud
authors: A
authors: Groen
authors: Andy
authors: A
authors: Christoforou
authors: Julie
authors: J
authors: Howard
authors: Nick
authors: N
authors: Bond
authors: Sabine
authors: S
authors: Bahn
authors: Kathryn S
authors: KS
authors: Lilley
mesh_terms: D015415:Biomarkers / Q000097:blood*; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D020543:Proteome / Q000032:analysis*; D040901:Proteomics / Q000379:methods*
chemical_list: D015415:Biomarkers; D020543:Proteome