title: Regulation of atrophin by both strands of the mir-8 precursor.
abstract: In Drosophila melanogaster, miR-8-3p regulates mRNA levels of atrophin, a factor involved in neuromotor coordination, and we found that Blattella germanica with suppressed atrophin showed motor problems. Bionformatic predictions and luciferase-reporter tests indicated that B. germanica atrophin mRNA contains target sites for miR-8-3p and miR-8-5p. Suppression of miR-8-3p or miR-8-5p appeared to increase atrophin mRNA. The effects of suppression of Argonaute (AGO) 1 or AGO2 expression on miR-8-3p and miR-8-5p suggested that miR-8-3-p might predominantly bind to AGO1, whereas miR-8-5p might bind to a moderate extent to both AGO1 and AGO2 in the respective RNA-induced silencing complexes (RISCs). We propose that the interplay of miR-8-3p, miR-8-5p, AGO1 and AGO2, maintain the appropriate levels of atrophin mRNA. This would be the first example of two strands of the same miRNA precursor regulating a single transcript.
authors: Institute of Evolutionary Biology (CSIC - Universitat Pompeu Fabra), Passeig Maritim de la Barceloneta 37, 0803 Barcelona, Spain.
authors: Mercedes
authors: M
authors: Rubio
authors: Raúl
authors: R
authors: Montañez
authors: Lidia
authors: L
authors: Perez
authors: Marco
authors: M
authors: Milan
authors: Xavier
authors: X
authors: Belles
mesh_terms: D000818:Animals; D003058:Cockroaches / Q000235:genetics* / Q000378:metabolism; D005786:Gene Expression Regulation; D019476:Insect Proteins / Q000235:genetics* / Q000378:metabolism; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: D019476:Insect Proteins; D035683:MicroRNAs; D009419:Nerve Tissue Proteins
keywords: Argonaute; Atrophin; Blattella; Drosophila; Metamorphosis; miR-8; miRNA passenger strand