title: Efficient in vivo depletion of CD8(+) T lymphocytes in common marmosets by novel CD8 monoclonal antibody administration.
abstract: In order to directly demonstrate the roles of CD8(+) T lymphocytes in non-human primates, in vivo depletion of the CD8(+) T cells by administration of a CD8-specific monoclonal antibody (mAb) is one of the crucial techniques. Recently, the common marmoset (Callithrix jacchus), which is classified as a New World monkey, has been shown useful as an experimental animal model for various human diseases such as multiple sclerosis, Parkinson's disease and a number of infectious diseases. Here we show that an anti-marmoset CD8 mAb 6F10, which we have recently established, efficiently depletes the marmoset CD8(+) T lymphocytes in vivo, i.e., the administration of 6F10 induces drastic and specific reduction in the ratio of the CD8(+) T cell subset for at least three weeks or longer. Our finding will help understand the pivotal role of CD8(+) T cells in vivo in the control of human diseases.
authors: Tsukuba Primate Research Center, National Institute of Biomedical Innovation, Hachimandai, Tsukuba, Ibaraki 305-0843, Japan; Primate Research Institute, Kyoto University, Inuyama, Aichi 484-8506, Japan. Electronic address: yoshida.tomoyuki.4w@kyoto-u.ac.jp.
authors: Tomoyuki
authors: T
authors: Yoshida
authors: Saori
authors: S
authors: Suzuki
authors: Yuki
authors: Y
authors: Iwasaki
authors: Akihisa
authors: A
authors: Kaneko
authors: Akatsuki
authors: A
authors: Saito
authors: Yuki
authors: Y
authors: Enomoto
authors: Atsunori
authors: A
authors: Higashino
authors: Akino
authors: A
authors: Watanabe
authors: Juri
authors: J
authors: Suzuki
authors: Kenichi
authors: K
authors: Inoue
authors: Teiko
authors: T
authors: Kuroda
authors: Masahiko
authors: M
authors: Takada
authors: Ryoji
authors: R
authors: Ito
authors: Mamoru
authors: M
authors: Ito
authors: Hirofumi
authors: H
authors: Akari
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000008:administration & dosage*; D016827:CD8 Antigens / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D002144:Callithrix / Q000276:immunology*; D002478:Cells, Cultured; D004195:Disease Models, Animal; D006801:Humans; D008212:Lymphocyte Depletion; D009103:Multiple Sclerosis / Q000276:immunology*; D016176:T-Lymphocyte Subsets / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D016827:CD8 Antigens
keywords: CD8 T lymphocyte; Common marmoset; In vivo depletion; Monoclonal antibody