title: The osteogenic activity of human mandibular fracture haematoma-derived cells is stimulated by low-intensity pulsed ultrasound in vitro.
abstract: Low intensity pulsed ultrasound (LIPUS) stimulation is a clinically established treatment method used to accelerate long bone fracture healing; however, this method is currently not applied to mandibular fractures. In this study, we investigated the effects of LIPUS on human mandibular fracture haematoma-derived cells (MHCs) in order to explore the possibility of applying LIPUS treatment to mandibular fractures. MHCs were isolated from five patients. The cells were divided into two groups: (1) LIPUS (+) group: MHCs cultured in osteogenic medium with LIPUS treatment; and (2) LIPUS (-) group: MHCs cultured in osteogenic medium without LIPUS treatment. The osteogenic differentiation potential and proliferation of the MHCs were compared between the two groups. The waveform used was equal to the wave conditions of a clinical fracture healing system. The gene expression levels of ALP, OC, Runx2, OSX, OPN, and PTH-R1 and mineralization were increased in the LIPUS (+) group compared to the LIPUS (-) group. There were no significant differences in cell proliferation between the two groups. These findings demonstrate the significant effects of LIPUS on the osteogenic differentiation of MHCs. This study provides significant evidence for the potential usefulness of the clinical application of LIPUS to accelerate mandibular fracture healing.
authors: Department of Oral and Maxillofacial Surgery, Kobe University Graduate School of Medicine, Kobe, Japan.
authors: Y
authors: Y
authors: Imai
authors: Department of Oral and Maxillofacial Surgery, Kobe University Graduate School of Medicine, Kobe, Japan. Electronic address: hasetaku@med.kobe-u.ac.jp.
authors: T
authors: T
authors: Hasegawa
authors: Department of Oral and Maxillofacial Surgery, Kobe University Graduate School of Medicine, Kobe, Japan.
authors: D
authors: D
authors: Takeda
authors: Department of Oral and Maxillofacial Surgery, Kobe University Graduate School of Medicine, Kobe, Japan.
authors: M
authors: M
authors: Akashi
authors: Department of Orthopedic Surgery, Kobe University Graduate School of Medicine, Kobe, Japan.
authors: S Y
authors: SY
authors: Lee
authors: Department of Orthopedic Surgery, Kobe University Graduate School of Medicine, Kobe, Japan.
authors: T
authors: T
authors: Niikura
authors: Department of Oral and Maxillofacial Surgery, Kobe University Graduate School of Medicine, Kobe, Japan.
authors: Y
authors: Y
authors: Shibuya
authors: Department of Orthopedic Surgery, Kobe University Graduate School of Medicine, Kobe, Japan.
authors: M
authors: M
authors: Kurosaka
authors: Department of Oral and Maxillofacial Surgery, Kobe University Graduate School of Medicine, Kobe, Japan.
authors: T
authors: T
authors: Komori
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D005260:Female; D017102:Fracture Healing / Q000502:physiology*; D015870:Gene Expression; D006406:Hematoma / Q000473:pathology; D006801:Humans; D008297:Male; D008337:Mandibular Fractures / Q000473:pathology / Q000628:therapy*; D008875:Middle Aged; D010012:Osteogenesis / Q000502:physiology*; D060888:Real-Time Polymerase Chain Reaction; D014464:Ultrasonic Therapy / Q000379:methods*; D014465:Ultrasonics
keywords: haematoma; low intensity pulsed ultrasound (LIPUS); mandibular fracture; osteogenic activity