title: Comparative study of the effects of laser photobiomodulation and extract of Brassica oleracea on skin wounds in wistar rats: A histomorphometric study.
abstract: The objective of this study was to investigate the effect of a photobiomodulation laser and Brassica oleracea on tissue morphology in skin wounds. The parameters analyzed were type I and III collagen fibers, and thickness and surface density of the epithelial tissue, as well as how quickly the wound closed. Five skin wounds 12mm in diameter were made on the backs of the animals, which were randomized into four groups (8 animals each). Saline Group: 0.9% saline solution; Ointment Group (extract of Cabbage, B. oleracea, 10% lanolin); Balsam Group (10% glycolic extract of B. oleracea emulsion oil); L60 Group (laser GaAsAl 60J/cm(2)). The applications were made daily during a 20-day treatment, and every 4 days tissue from different wounds was removed. The reduction in the size of the wounds on the 4th, 8th, 12th and 16th days was significantly greater in the treated groups compared to the control group. At all the time points analyzed, there was a greater proportion of collagen in the Balsam and L60 groups (p<0.05). There was also a greater proliferation of epithelial cells in the L60 and Balsam groups after 20 days of treatment (p<0.05). The healing extract and laser 60j/cm(2) exerted a great effect on collagen proliferation in stimulating scar tissue maturation.
authors: Department of Basic Science Area Health, Federal University of Juiz de Fora, Brazil. Electronic address: reggysvilela@yahoo.com.br.
authors: Reggiani Vilela
authors: RV
authors: Gonçalves
authors: Mariáurea Matias
authors: MM
authors: Sarandy
authors: Sérgio Luis Pinto
authors: SL
authors: da Matta
authors: Rômulo Dias
authors: RD
authors: Novaes
authors: Marcus Vinicius de Mello
authors: MV
authors: Pinto
mesh_terms: D000818:Animals; D001937:Brassica; D053685:Laser Therapy / Q000379:methods*; D008297:Male; D018462:Photobiology / Q000379:methods; D008517:Phytotherapy / Q000379:methods*; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D014945:Wound Healing / Q000187:drug effects* / Q000528:radiation effects*
chemical_list: D010936:Plant Extracts
keywords: Brassica oleracea; Collagen; Laser therapy; Skin wounds