title: Nuclear localization of huntingtin during spermatogenesis.
abstract: Huntingtin is a ubiquitous cytoplasmic protein. Mutant huntingtin causes Huntington's disease and its intranuclear inclusion is associated with cytotoxicity. Nuclear localization of normal huntingtin is detected in the oocyte up to 2.5 days post coitum. Therefore, huntingtin is expected to reside in the nucleus even before fertilization. The present study determined normal huntingtin distribution during spermatogenesis. Testicles from an adult male Sprague-Dawley rat were stained with anti-huntingtin antibody and nuclear counterstaining was performed with 4',6-diamidino-2-phenylindole. Concerning nuclear localization, huntingtin was detected in the spermatids, whereas predominant cytoplasmic localization of it was evident in the spermatogonia. Between the primary and secondary spermatocytes, huntingtin appeared to be delocalized in the nuclei when meiosis occurred. The findings provide evidence that normal huntingtin is transported to the nuclear compartment during the meiotic stage of spermatogenesis.
authors: Department of Neurology, Seoul National University Hospital, 101 Daehakro, Chongnogu, Seoul, 110-744, Korea.
authors: Wooseok
authors: W
authors: Im
authors: Jinyoung
authors: J
authors: Chung
authors: Soon-Tae
authors: ST
authors: Lee
authors: Kon
authors: K
authors: Chu
authors: Min-Wook
authors: MW
authors: Kim
authors: Manho
authors: M
authors: Kim
mesh_terms: D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism*; D000071058:Huntingtin Protein; D008297:Male; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D009687:Nuclear Proteins / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013087:Spermatids / Q000648:ultrastructure; D013091:Spermatogenesis / Q000502:physiology*; D013737:Testis / Q000378:metabolism*
chemical_list: C499524:Htt protein, rat; D000071058:Huntingtin Protein; D009419:Nerve Tissue Proteins; D009687:Nuclear Proteins
references: Eur J Med Genet. 2011 Jul-Aug;54(4):e413-8
references: 21540131
references: J Biol Chem. 2002 Mar 1;277(9):7466-76
references: 11739372
references: Science. 2001 Mar 23;291(5512):2423-8
references: 11264541
references: Science. 2001 Jul 20;293(5529):493-8
references: 11408619
references: Proc Natl Acad Sci U S A. 2000 Jun 6;97(12):6763-8
references: 10823891
references: Proc Natl Acad Sci U S A. 2003 Jul 22;100(15):8834-8
references: 12857955
references: Trends Cell Biol. 2009 Apr;19(4):147-55
references: 19269181
references: Int J Dev Neurosci. 2006 Feb;24(1):81-5
references: 16289942
references: Cell. 1993 Mar 26;72(6):971-83
references: 8458085
references: Science. 1997 Sep 26;277(5334):1990-3
references: 9302293