title: Cold shock proteins contribute to the regulation of listeriolysin O production in Listeria monocytogenes.
abstract: Cold shock proteins (Csps) are multifunctional nucleic acid binding proteins used to regulate a wide range of gene expression responses in bacteria. We report here that Csps regulate the production of the pore-forming cytolysin listeriolysin (LLO) and hemolysis phenotypes in Listeria monocytogenes. A triple csp gene deletion mutant incapable of producing any Csps, as well as double csp gene deletion mutants only producing either CspA or CspD, caused less hemolysis and produced lower LLO concentration. On the other hand, another double csp gene deletion mutant that produces CspB retained hemolysis and LLO production levels that are similar to the parental wild-type strain. Transcription analysis showed that in absence of all three csp genes or cspB alone, L. monocytogenes cells have decreased levels of hly gene transcripts, which code for the synthesis of LLO proteins. A comparative examination of mRNA stability showed that hly transcripts were more rapidly degraded in L. monocytogenes triple csp gene deletion mutant cells that are not capable of producing Csps. Overall, our results indicate that Csps, in particular CspB, are important components of gene expression regulatory mechanisms that promote efficient LLO production and hence virulence responses of L. monocytogenes.
authors: Institute for Food Safety and Hygiene, Vetsuisse Faculty University of Zurich, Zurich , Switzerland .
authors: Kerstin
authors: K
authors: Schärer
authors: Roger
authors: R
authors: Stephan
authors: Taurai
authors: T
authors: Tasara
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D001427:Bacterial Toxins / Q000235:genetics / Q000378:metabolism*; D057773:Cold Shock Proteins and Peptides / Q000235:genetics / Q000378:metabolism*; D017353:Gene Deletion; D015964:Gene Expression Regulation, Bacterial; D006360:Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism*; D006460:Hemolysin Proteins / Q000235:genetics / Q000378:metabolism*; D006461:Hemolysis; D008089:Listeria monocytogenes / Q000235:genetics / Q000472:pathogenicity / Q000502:physiology*; D008088:Listeriosis; D009154:Mutation; D010641:Phenotype; D020871:RNA Stability; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014774:Virulence; D037521:Virulence Factors
chemical_list: D001426:Bacterial Proteins; D001427:Bacterial Toxins; D057773:Cold Shock Proteins and Peptides; C088098:CspD protein, bacteria; D006360:Heat-Shock Proteins; D006460:Hemolysin Proteins; D012333:RNA, Messenger; D037521:Virulence Factors; C062260:cold shock protein CS7.4, Bacteria; C077188:cold-shock protein CspB, Bacteria; C038705:hlyA protein, Listeria monocytogenes