title: Reversion of mouse postimplantation epiblast stem cells to a naïve pluripotent state by modulation of signalling pathways.
abstract: Mouse postimplantation epiblast cultured in activin and basic fibroblast growth factor gives rise to continuously growing epiblast stem cells (EpiSCs) that share key properties with postimplantation epiblast, such as DNA methylation and an inactive X-chromosome. EpiSCs also show a distinct gene expression profile compared to embryonic stem cells (ESCs) derived from preimplantation blastocysts, and do not contribute efficiently to chimeras. EpiSCs can, however, revert to pluripotent ESC-like cells upon exposure to leukemia inhibitory factor-Stat3 signalling on feeder cells. Here we describe a protocol for the establishment of EpiSCs and their reversion to ESCs.
authors: Wellcome Trust/Cancer Research UK Gurdon Institute, University of Cambridge, Cambridge, UK.
authors: Astrid
authors: A
authors: Gillich
authors: Siqin
authors: S
authors: Bao
authors: M Azim
authors: MA
authors: Surani
mesh_terms: D028341:Activins / Q000494:pharmacology; D000818:Animals; D001755:Blastocyst / Q000166:cytology*; D018929:Cell Culture Techniques / Q000379:methods*; D019175:DNA Methylation / Q000235:genetics; D053595:Embryonic Stem Cells / Q000166:cytology*; D005347:Fibroblasts / Q000166:cytology; D005855:Germ Layers / Q000166:cytology*; D051379:Mice; D039904:Pluripotent Stem Cells / Q000166:cytology; D015398:Signal Transduction
chemical_list: D028341:Activins