title: Association between resistin +299A/A genotype and nonalcoholic fatty liver disease in Chinese patients with type 2 diabetes mellitus.
abstract: OBJECTIVE
  To investigate the relationship between the resistin intronic +299G/A polymorphism and nonalcoholic fatty liver disease (NAFLD) in patients with type 2 diabetes mellitus (T2DM).
  
  
  METHODS
  We selected 738 T2DM patients, including 395 with NAFLD and 343 without fatty liver disease, as well as 279 healthy control individuals, and analyzed their resistin +299G/A polymorphism genotype by polymerase chain reaction-restriction fragment length polymorphism.
  
  
  RESULTS
  Plasma resistin levels in T2DM patients with NAFLD were at the highest (P<0.05). The frequency of AA genotype at the +299 site of the resistin gene in patients with concurrent T2DM combined with NAFLD was significantly different from that in the control (P<0.05). The AA genotype was found to be associated with a 1.80-fold increased risk for T2DM combined with NAFLD, 2.05-fold increased risk for obesity and 2.37-fold increased risk for obesity of abdominal type compared to the GG (P<0.05, respectively). The multivariate non-conditional logistic regression model analysis further shows that the AA genotype is a risk factor for the development of NAFLD in T2DM patients (OR, 2.32; 95% CI, 1.05-4.68; P<0.05).
  
  
  CONCLUSIONS
  The resistin +299AA genotype may be associated with increases in the risk of the NAFLD development in T2DM patients.
authors: Department of Endocrinology, Affiliated Yantai Hospital of Binzhou Medical University, Yantai, Shandong, China. Electronic address: dhbandzly0603@126.com.
authors: Ling-Yun
authors: LY
authors: Zhang
authors: Yong-Jun
authors: YJ
authors: Jin
authors: Qing-Song
authors: QS
authors: Jin
authors: Liang-Yan
authors: LY
authors: Lin
authors: Dong-Dong
authors: DD
authors: Zhang
authors: Li-Li
authors: LL
authors: Kong
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D002681:China; D003924:Diabetes Mellitus, Type 2 / Q000150:complications / Q000235:genetics*; D005234:Fatty Liver / Q000150:complications / Q000235:genetics*; D005260:Female; D056726:Genetic Association Studies; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D065626:Non-alcoholic Fatty Liver Disease; D020641:Polymorphism, Single Nucleotide; D052243:Resistin / Q000235:genetics*
chemical_list: C499624:RETN protein, human; D052243:Resistin
keywords: ALT; AST; Adipocytokine; BMI; C/EBP; CCAAT/enhancer binding protein; FBG; FINS; HDL; HSCs; IRS; Insulin resistance; LDL; MAPK; NAFLD; NASH; Non-alcoholic fatty liver disease; PI-3K; PPAR; RETN; Resistin; SNPs; SOCS; Single nucleotide polymorphism; T2DM; TC; TG; TNF; Triglyceride; WHR; alanine aminotransferase; aspartate transaminase; body mass index; fasting blood glucose; fasting insulin; gamma-glutamyl transferase; hematopoietic stem cells; high density lipoprotein; insulin receptor substrate; low density lipoprotein; mitogen-activated protein kinase; nonalcoholic fatty liver disease; nonalcoholic steatohepatitis; peroxisome proliferator-activated receptor; phosphatidyl inositol 3-kinase; single nucleotide polymorphisms; suppressor of cytokine signaling; total cholesterol; tumor necrosis factor; type 2 diabetes mellitus; waist-to-hip ratio; γ-GT