title: Antibiofilm activity of coconut (Cocos nucifera Linn.) husk fibre extract.
abstract: In this study, antibiofilm activity of coconut husk extract (CHE) was tested by various assays in the laboratory. The effects of CHE on extracellular polymeric substance (EPS) production, hydrophobicity and adhesion ability of Pseudomonas sp., Alteromonas sp. and Gallionella sp. and the antimicrobial activity of the extract against these bacteria were assessed. CHE was found to possess antibacterial activity against all the bacterial strains and affected the EPS production. The CHE affected the growth of the biofilm-forming bacteria in a culture medium. The hydrophobicity of the bacterial cells was also changed due to the CHE treatment. The active compound of the CHE was characterised by thin-layer chromatography (TLC), high performance liquid chromatography (HPLC) and fourier transform infrared (FT-IR) analysis. HPLC spectrum showed a single peak and the FT-IR spectrum indicated the presence of an OH-group-containing compound in the extract. In conclusion the CHE could be used as a source for the isolation of antifouling compounds.
authors: International Center for Nanobiotechnology, Center for Marine Science and Technology, Manonmanium Sundaranar University, Rajakkamangalam 629502, Kanyakumari-Dist, Tamil Nadu, India.
authors: N
authors: N
authors: Viju
authors: S
authors: S
authors: Satheesh
authors: S G P
authors: SG
authors: Vincent
keywords: Adhesion; Biofilm; Biofouling; Extracellular polymeric substance; Polyphenol
references: Int J Food Microbiol. 1998 Jun 30;42(1-2):9-27
references: 9706794
references: Am J Clin Nutr. 2005 Jan;81(1 Suppl):230S-242S
references: 15640486
references: Biol Trace Elem Res. 1998 Winter;66(1-3):227-35
references: 10050922
references: Pharmacol Rev. 2000 Dec;52(4):673-751
references: 11121513
references: Microbiol Mol Biol Rev. 2000 Dec;64(4):847-67
references: 11104821
references: Appl Environ Microbiol. 2003 Apr;69(4):2245-52
references: 12676707
references: Nat Prod Rep. 2004 Feb;21(1):94-104
references: 15039837
references: FEMS Microbiol Rev. 1999 Apr;23(2):179-230
references: 10234844
references: Biofouling. 2005;21(2):109-19
references: 16109600
references: Mar Biotechnol (NY). 2005 May-Jun;7(3):142-62
references: 15776313
references: Res Microbiol. 2002 Dec;153(10):647-52
references: 12558183
references: Biofouling. 2007;23(3-4):171-7
references: 17653928
references: J Biol Chem. 1951 Nov;193(1):265-75
references: 14907713
references: J Appl Microbiol. 2001 Apr;90(4):494-507
references: 11309059