title: Protease inhibitor from Moringa oleifera with potential for use as therapeutic drug and as seafood preservative.
abstract: Protease inhibitors are well known to have several applications in medicine and biotechnology. Several plant sources are known to return potential protease inhibitors. In this study plants belonging to different families of Leguminosae, Malvaceae, Rutaceae, Graminae and Moringaceae were screened for the protease inhibitor. Among them Moringa oleifera, belonging to the family Moringaceae, recorded high level of protease inhibitor activity after ammonium sulfate fractionation. M. oleifera, which grows throughout most of the tropics and having several industrial and medicinal uses, was selected as a source of protease inhibitor since so far no reports were made on isolation of the protease inhibitor. Among the different parts of M. oleifera tested, the crude extract isolated from the mature leaves and seeds showed the highest level of inhibition against trypsin. Among the various extraction media evaluated, the crude extract prepared in phosphate buffer showed maximum recovery of the protease inhibitor. The protease inhibitor recorded high inhibitory activity toward the serine proteases thrombin, elastase, chymotrypsin and the cysteine proteases cathepsin B and papain which have more importance in pharmaceutical industry. The protease inhibitor also showed complete inhibition of activities of the commercially available proteases of Bacillus licheniformis and Aspergillus oryzae. However, inhibitory activities toward subtilisin, esperase, pronase E and proteinase K were negligible. Further, it was found that the protease inhibitor could prevent proteolysis in a commercially valuable shrimp Penaeus monodon during storage indicating the scope for its application as a seafood preservative. This is the first report on isolation of a protease inhibitor from M. oleifera.
authors: Microbial Technology Laboratory, Department of Biotechnology, Cochin University of Science and Technology, Cochin 682022, India.
authors: B
authors: B
authors: Bijina
authors: Sreeja
authors: S
authors: Chellappan
authors: Jissa G
authors: JG
authors: Krishna
authors: Soorej M
authors: SM
authors: Basheer
authors: K K
authors: KK
authors: Elyas
authors: Ali H
authors: AH
authors: Bahkali
authors: M
authors: M
authors: Chandrasekaran
keywords: Moringa oleifera; Protease inhibitor; Seafood preservative; Therapeutic proteases
references: J Ethnopharmacol. 1991 Jul;33(3):213-6
references: 1921416
references: Antimicrob Agents Chemother. 2002 Aug;46(8):2633-5
references: 12121944
references: Phytochemistry. 1996 Mar;41(4):1017-22
references: 8728712
references: C R Acad Sci III. 1985;300(13):471-4
references: 3924358
references: Antonie Van Leeuwenhoek. 1985;51(2):219-25
references: 4037785
references: Lett Appl Microbiol. 2000 Sep;31(3):259-64
references: 10972741
references: Methods Biochem Anal. 2005;46:1-265
references: 16350889
references: J Ethnopharmacol. 2010 Jul 6;130(1):183-6
references: 20435128
references: J Cell Sci. 1998 Mar;111 ( Pt 5):597-606
references: 9454733
references: Hoppe Seylers Z Physiol Chem. 1966;345(2):150-67
references: 5989600
references: Lloydia. 1971 Dec;34(4):386-425
references: 5173435
references: Cancer Res. 1991 Apr 15;51(8):2107-12
references: 2009530
references: Insect Mol Biol. 2004 Dec;13(6):649-57
references: 15606813
references: Antonie Van Leeuwenhoek. 2005 Jan;87(1):59-63
references: 15726292
references: Proc Natl Acad Sci U S A. 1990 Apr;87(8):3032-6
references: 1970179
references: Curr Top Med Chem. 2006;6(4):317-29
references: 16611145
references: Bioresour Technol. 2002 Sep;84(2):165-9
references: 12139333
references: J Insect Physiol. 2002 Jul;48(7):701-706
references: 12770064
references: Methods Enzymol. 1981;80 Pt C:737-54
references: 6176834
references: J Protein Chem. 1996 Feb;15(2):219-29
references: 8924206
references: Comp Biochem Physiol C Toxicol Pharmacol. 2003 Apr;134(4):481-90
references: 12727298
references: Methods Enzymol. 1976;45:475-85
references: 1012011
references: J Biochem. 1995 Feb;117(2):432-7
references: 7608135
references: J Gen Physiol. 1947 Mar 20;30(4):291-310
references: 19873496
references: J Insect Physiol. 2005 Jan;51(1):75-86
references: 15686649
references: Annu Rev Biochem. 1980;49:593-626
references: 6996568
references: Curr Opin Microbiol. 1998 Aug;1(4):455-60
references: 10066510
references: Bioorg Med Chem. 2010 Sep 1;18(17):6598-602
references: 20685125
references: Ann Biochem Exp Med. 1961 Dec;21:359-66
references: 13919894
references: J Biol Chem. 1951 Nov;193(1):265-75
references: 14907713