title: Spectroscopic, viscositic and molecular modeling studies on the interaction of 3'-azido-daunorubicin thiosemicarbazone with DNA.
abstract: A new daunorubicin has been synthesized and structurally characterized. The interaction of native calf thymus DNA (ctDNA) with 3'-azido-daunorubicin thiosemicarbazone (ADNRT) was investigated under simulated physiological conditions by multi-spectroscopic techniques, viscometric measurements and molecular modeling study. It concluded that ADNRT could intercalate into the base pairs of ctDNA, and the fluorescence quenching by ctDNA was static quenching type. Thermodynamic parameters calculated suggested that the binding of ADNRT to ctDNA was mainly driven by hydrophobic interactions. The relative viscosity of ctDNA increased with the addition of ADNRT, which confirmed the intercalation mode. Furthermore, molecular modeling studies corroborate the above experimental results.
authors: School of Chemistry and Chemical Engineering, Key Laboratory of Green Chemical Media and Reactions, Ministry of Education, Henan Normal University, Xinxiang, 453007, China, fenglingcui@hotmail.com.
authors: Fengling
authors: F
authors: Cui
authors: Qingfeng
authors: Q
authors: Liu
authors: Hongxia
authors: H
authors: Luo
authors: Guisheng
authors: G
authors: Zhang
mesh_terms: D000818:Animals; D002417:Cattle; D004247:DNA / Q000737:chemistry*; D003630:Daunorubicin / Q000031:analogs & derivatives* / Q000737:chemistry; D008958:Models, Molecular; D013050:Spectrometry, Fluorescence; D013882:Thiosemicarbazones / Q000737:chemistry*; D014783:Viscosity
chemical_list: C000592557:3'-azido-daunorubicin thiosemicarbazone; D013882:Thiosemicarbazones; D004247:DNA; C427203:calf thymus DNA; D003630:Daunorubicin
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