title: Age-related modulation of the effects of obesity on gene expression profiles of mouse bone marrow and epididymal adipocytes.
abstract: This study aimed to characterize and compare the effects of obesity on gene expression profiles in two distinct adipose depots, epididymal and bone marrow, at two different ages in mice. Alterations in gene expression were analyzed in adipocytes isolated from diet-induced obese (DIO) C57BL/6J male mice at 6 and 14 months of age and from leptin deficient mice (ob/ob) at 6 months of age using microarrays. DIO affected gene expression in both depots at 6 and 14 months, but more genes were altered in epididymal than bone marrow adipocytes at each age and younger mice displayed more changes than older animals. In epididymal adipocytes a total of 2789 (9.6%) genes were differentially expressed at 6-months with DIO, whereas 952 (3.3%) were affected at 14-months. In bone marrow adipocytes, 347 (1.2%) genes were differentially expressed at 6-months with DIO, whereas only 189 (0.66%) were changed at 14-months. 133 genes were altered by DIO in both fat depots at 6-months, and 37 genes at 14-months. Only four genes were altered in both depots at both ages with DIO. Bone marrow adipocytes are less responsive to DIO than epididymal adipocytes and the response of both depots to DIO declines with age. This loss of responsiveness with age is likely due to age-associated changes in expression of genes related to adipogenesis, inflammation and mitochondrial function that are similar to and obscure the changes commonly associated with DIO. Patterns of gene expression were generally similar in epididymal adipocytes from ob/ob and DIO mice; however, several genes were differentially expressed in bone marrow adipocytes from ob/ob and DIO mice, perhaps reflecting the importance of leptin signaling for bone metabolism. In conclusion, obesity affects age-associated alterations in gene expression in both epididymal and bone marrow adipocytes regardless of diet or genetic background.
authors: Division of Endocrinology, Stanford University, Stanford, California, United States of America.
authors: Li-Fen
authors: LF
authors: Liu
authors: Wen-Jun
authors: WJ
authors: Shen
authors: Masami
authors: M
authors: Ueno
authors: Shailja
authors: S
authors: Patel
authors: Salman
authors: S
authors: Azhar
authors: Fredric B
authors: FB
authors: Kraemer
mesh_terms: D017667:Adipocytes / Q000378:metabolism / Q000473:pathology*; D000375:Aging / Q000235:genetics* / Q000378:metabolism / Q000473:pathology*; D000818:Animals; D001854:Bone Marrow Cells / Q000473:pathology*; D059305:Diet, High-Fat / Q000009:adverse effects; D004822:Epididymis / Q000473:pathology*; D020738:Leptin / Q000172:deficiency; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009765:Obesity / Q000235:genetics / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology*; D020411:Oligonucleotide Array Sequence Analysis; D059467:Transcriptome
chemical_list: D020738:Leptin
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