title: Optimization of Solid-State Fermentation for Improved Conidia Production of Beauveria bassiana as a Mycoinsecticide.
abstract: The production of conidia of entomopathogenic Beauveria bassiana by solid-state fermentation was studied for the development of a biocontrol agent against aphid Myzus persicae. The optimal conditions for conidia production on polished white rice were 40% moisture content, 25℃ culture temperature, 2-day-old seeding culture grown in 3% corn meal, 2% rice bran, 2% corn steep powder medium, initial conidia concentration of 10(7) conidia/g in the wet rice, 10% inoculum size, and use of a polyethylene bag as a container. The polyethylene bag containing inoculated rice was hand-shaken every 12 hr during fermentation. Using optimal conditions, the maximum conidia production obtained was 4.05 g conidia/100 g dry rice after 14 days of cultivation, a rate 2.83 times higher than conidia yield of pre-optimization.
authors: Department of Bioscience and Biotechnology, The University of Suwon, Hwaseong 445-743, Korea.
authors: Tuan Anh
authors: TA
authors: Pham
authors: Jeong Jun
authors: JJ
authors: Kim
authors: Keun
authors: K
authors: Kim
keywords: Beauveria bassiana; Conidia production; Optimal culture conditions; Solid-state fermentation
references: Mycobiology. 2009 Sep;37(3):218-24
references: 23983537
references: Mycobiology. 2008 Sep;36(3):183-9
references: 23997622