title: Reduction of adipogenesis and lipid accumulation by Taraxacum officinale (Dandelion) extracts in 3T3L1 adipocytes: an in vitro study.
abstract: In this in vitro study, we have investigated the ability of Taraxacum officinale (dandelion) to inhibit adipocyte differentiation and lipogenesis in 3T3-L1 preadipocytes. HPLC analysis of the three plant extracts used in this study-leaf and root extracts and a commercial root powder-identified caffeic and chlorogenic acids as the main phenolic constituents. Oil Red O staining and triglyceride levels analysis showed decreased lipid and triglyceride accumulation, respectively. Cytotoxicity was assessed with the MTT assay showing non-toxic effect among the concentrations tested. DNA microarray analysis showed that the extracts regulated the expression of a number of genes and long non-coding RNAs that play a major role in the control of adipogenesis. Taken together, our results indicate that the dandelion extracts used in this study may play a significant role during adipogenesis and lipid metabolism, and thus, supporting their therapeutic interest as potential candidates for the treatment of obesity.
authors: IMDEA Food Institute, Crta. Cantoblanco 8, 28049, Madrid, Spain.
authors: Marta
authors: M
authors: González-Castejón
authors: Belén
authors: B
authors: García-Carrasco
authors: Raquel
authors: R
authors: Fernández-Dacosta
authors: Alberto
authors: A
authors: Dávalos
authors: Arantxa
authors: A
authors: Rodriguez-Casado
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000187:drug effects* / Q000378:metabolism; D050156:Adipogenesis / Q000187:drug effects*; D000818:Animals; D019440:Anti-Obesity Agents / Q000494:pharmacology; D002109:Caffeic Acids / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects; D002726:Chlorogenic Acid / Q000494:pharmacology; D050356:Lipid Metabolism / Q000187:drug effects*; D051379:Mice; D009765:Obesity; D010936:Plant Extracts / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry; D031205:Taraxacum / Q000737:chemistry*; D014280:Triglycerides / Q000378:metabolism
chemical_list: D019440:Anti-Obesity Agents; D002109:Caffeic Acids; D010936:Plant Extracts; D014280:Triglycerides; D002726:Chlorogenic Acid; C040048:caffeic acid
keywords: 3 T3-L1 cells; adipogenesis; dandelion; differentiation; long noncoding RNAs; obesity; phytochemicals