title: Characterisation of class I and II α-mannosidases from Drosophila melanogaster.
abstract: Homology searches indicated that up to five class I α-mannosidases (glycohydrolase family 47) and eight class II α-mannosidases (glycohydrolase family 38) are encoded by the fruitfly (Drosophila melanogaster) genome. Selected example mannosidases were expressed in secreted form using the yeast Pichia pastoris. A number of characteristics of these enzymes were determined with p-nitrophenyl-α-mannoside as substrate; particularly striking were the low optima (pH 5) of three class II mannosidases most closely related to known lysosomal mannosidases and the distinct Co(II)-requirement of a mannosidase previously named ManIIb. Some of the recombinant mannosidases were demonstrably active towards oligomannosidic glycans, specifically, the Co(II)-requiring ManIIb, two 'acidic' mannosidases and the class I mas-1 mannosidase. Other than previous characterisations of the well-known Golgi mannosidase II, this is the first study summarising various properties of recombinant mannosidases from the fruitfly.
authors: Chemický ústav, Slovenská akadémia vied, Dúbravská cesta 9, 845 38, Bratislava, Slovakia.
authors: Ivana
authors: I
authors: Nemčovičová
authors: Sergej
authors: S
authors: Šesták
authors: Dubravko
authors: D
authors: Rendić
authors: Margita
authors: M
authors: Plšková
authors: Ján
authors: J
authors: Mucha
authors: Iain B H
authors: IB
authors: Wilson
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D029721:Drosophila Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D004331:Drosophila melanogaster / Q000201:enzymology*; D007700:Kinetics; D008969:Molecular Sequence Data; D011134:Polysaccharides / Q000378:metabolism; D013379:Substrate Specificity; D043323:alpha-Mannosidase / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism
chemical_list: D029721:Drosophila Proteins; D011134:Polysaccharides; D043323:alpha-Mannosidase
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