title: Luffa cylindrica and phytosterols bioconversion: from shake flask to jar bioreactor.
abstract: Bioconversion of lipophilic compounds poorly soluble in water, such as sterols, required the use of chemicals and solubilizing agents. On the other hand, it was shown that immobilization of Mycobacterium species on the dried fruit of Luffa cylindrica (DFLC) allows a close interaction between immobilized cells and cholesterol particles and increases by then the product's yield. In this work, the use of DFLC in a 5-l jar bioreactor with phytosterols mixture (1 g/l) as substrate was assessed without addition of any chemicals or solubilizing agents. DFLC increased by a factor of four the volumetric productivity of androstenones (0.08 g/l day). Products were accumulated in the aqueous medium while substrates remained on the fibers of DFLC. This observation lets envisage a green semi-continuous process of androstenone production. DFLC has no influence on cell growth, and is moreover natural, inexpensive, non-toxic, and mechanically strong.
authors: Laboratoire de Chimie Organique et Bioorganique, Institut de Recherche Jean-Baptiste Donnet, Université de Haute Alsace, EA4566, 3 rue Alfred Werner, 68093, Mulhouse, France.
authors: Hamid
authors: H
authors: Bou Saab
authors: Samuel
authors: S
authors: Fouchard
authors: Anna
authors: A
authors: Boulanger
authors: Pierre
authors: P
authors: Llopiz
authors: Serge
authors: S
authors: Neunlist
mesh_terms: D000736:Androstenes / Q000378:metabolism; D019149:Bioreactors / Q000191:economics; D001711:Biotransformation; D018914:Cells, Immobilized / Q000378:metabolism; D002784:Cholesterol / Q000378:metabolism; D005638:Fruit / Q000191:economics / Q000378:metabolism / Q000648:ultrastructure; D029776:Luffa / Q000378:metabolism / Q000648:ultrastructure; D009161:Mycobacterium / Q000166:cytology / Q000378:metabolism*; D010840:Phytosterols / Q000096:biosynthesis / Q000378:metabolism*; D012995:Solubility
chemical_list: D000736:Androstenes; D010840:Phytosterols; D002784:Cholesterol
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