title: Preparation of 20-HETE using multifunctional enzyme type 2-negative Starmerella bombicola.
abstract: The metabolism of arachidonic acid (ARA) by cytochrome P450 ω/ω-1-hydroxylases leads to the formation of 20-hydroxyeicosatetraenoic acid (20-HETE), which is an important lipid-signaling molecule involved in regulation of vascular tone, angiogenesis, and inflammation. Development of a simple method to prepare 20-HETE would greatly facilitate the investigation of its biological activities. The nonpathogenic yeast Starmerella bombicola has been shown to convert exogenously added arachidonic acid to 20-HETE via the biosynthetic pathway of sophorolipids; however, the yield was low. Here we demonstrate that genetic knockout of multifunctional enzyme type 2 (MFE-2), which is involved in the β-oxidation of fatty acids, significantly increases the yield of ARA conversion to 20-HETE and allows practical preparation of 20-HETE.
authors: Faculty of Bioscience Engineering, Ghent University, Ghent, Belgium.
authors: Inge
authors: I
authors: Van Bogaert
authors: Guodong
authors: G
authors: Zhang
authors: Jun
authors: J
authors: Yang
authors: Jun-Yan
authors: JY
authors: Liu
authors: Yonghao
authors: Y
authors: Ye
authors: Wim
authors: W
authors: Soetaert
authors: Bruce D
authors: BD
authors: Hammock
mesh_terms: D000818:Animals; D016718:Arachidonic Acid / Q000378:metabolism; D001203:Ascomycota / Q000201:enzymology*; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D055786:Gene Knockout Techniques; D006893:Hydroxyeicosatetraenoic Acids / Q000096:biosynthesis* / Q000378:metabolism / Q000494:pharmacology; D006900:Hydroxylation; D006913:Hydroxysteroid Dehydrogenases / Q000172:deficiency* / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D018919:Neovascularization, Physiologic / Q000187:drug effects; D010084:Oxidation-Reduction
chemical_list: D006893:Hydroxyeicosatetraenoic Acids; D016718:Arachidonic Acid; C055986:19-hydroxy-5,8,11,14-eicosatetraenoic acid; C055987:20-hydroxy-5,8,11,14-eicosatetraenoic acid; D003577:Cytochrome P-450 Enzyme System; D006913:Hydroxysteroid Dehydrogenases; C035507:3(17)-hydroxysteroid dehydrogenase
keywords: 20-hydroxyeicosatetraenoic acid; arachidonic acid; sophorolipids
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