title: Platelet a disintegrin and metallopeptidase 10 expression correlates with clock drawing test scores in Alzheimer's disease.
abstract: OBJECTIVE
  Earlier studies have demonstrated that a disintegrin and metallopeptidase 10 (ADAM10) levels are reduced in Alzheimer's disease (AD) patients compared with healthy subjects. The objective of this study was to evaluate whether platelet ADAM10 levels correlates with the clock drawing test (CDT) scores, which is a simple and a reliable measure of visuospatial ability and executive function in AD patients.
  
  
  METHODS
  Thirty elderly patients with probable AD and 25 healthy patients forming the control group, matched by age, gender, and educational level, were evaluated. Platelet proteins were analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis, and ADAM10 was identified by western blotting. The Spearman's correlation coefficient between ADAM10 and CDT was obtained for each group. The areas under the curves were used to compare the receiver operating characteristic curves.
  
  
  RESULTS
  The CDT scores and platelet ADAM10 expression were significantly different between patients with AD and controls and also along the disease's progression. In AD patients, there was a positive correlation between ADAM10 expression and CDT scores. Among non-AD subjects, no correlation was found. The combination of ADAM10 and CDT was significantly better to confirm the AD diagnosis than the AUCs of ADAM10 and CDT separately.
  
  
  CONCLUSIONS
  The association of blood-based biomarkers, such as ADAM10, and cognitive tests may be helpful for a more reliable AD diagnosis.
authors: Departamento de Gerontologia, UFSCar, São Carlos, São Paulo, Brazil.
authors: Patrícia Regina
authors: PR
authors: Manzine
authors: Elizabeth Joan
authors: EJ
authors: Barham
authors: Francisco Assis Carvalho
authors: FA
authors: Vale
authors: Heloisa Sobreiro
authors: HS
authors: Selistre-de-Araújo
authors: Sofia Cristina Iost
authors: SC
authors: Pavarini
authors: Márcia Regina
authors: MR
authors: Cominetti
mesh_terms: D051722:ADAM Proteins / Q000097:blood*; D000072197:ADAM10 Protein; D000368:Aged; D000369:Aged, 80 and over; D000544:Alzheimer Disease / Q000097:blood* / Q000175:diagnosis / Q000523:psychology; D053829:Amyloid Precursor Protein Secretases / Q000097:blood*; D019540:Area Under Curve; D001792:Blood Platelets / Q000378:metabolism*; D016022:Case-Control Studies; D019483:Disintegrins / Q000097:blood*; D056344:Executive Function / Q000502:physiology; D005260:Female; D006801:Humans; D008297:Male; D008565:Membrane Proteins / Q000097:blood*; D008875:Middle Aged; D009483:Neuropsychological Tests
chemical_list: D019483:Disintegrins; D008565:Membrane Proteins; D053829:Amyloid Precursor Protein Secretases; D051722:ADAM Proteins; D000072197:ADAM10 Protein; C491236:ADAM10 protein, human
keywords: ADAM10; Alzheimer's disease; biomarkers; clock drawing test; elderly patients; platelet