title: MAP65-1a positively regulates H2O2 amplification and enhances brassinosteroid-induced antioxidant defence in maize.
abstract: Brassinosteroid (BR)-induced antioxidant defence has been shown to enhance stress tolerance. In this study, the role of the maize 65 kDa microtubule-associated protein (MAP65), ZmMAP65-1a, in BR-induced antioxidant defence was investigated. Treatment with BR increased the expression of ZmMAP65-1a in maize (Zea mays) leaves and mesophyll protoplasts. Transient expression and RNA interference silencing of ZmMAP65-1a in mesophyll protoplasts further revealed that ZmMAP65-1a is required for the BR-induced increase in expression and activity of superoxide dismutase (SOD) and ascorbate peroxidase (APX). Both exogenous and BR-induced endogenous H2O2 increased the expression of ZmMAP65-1a. Conversely, transient expression of ZmMAP65-1a in maize mesophyll protoplasts enhanced BR-induced H2O2 accumulation, while transient silencing of ZmMAP65-1a blocked the BR-induced expression of NADPH oxidase genes and inhibited BR-induced H2O2 accumulation. Inhibiting the activity and gene expression of ZmMPK5 significantly prevented the BR-induced expression of ZmMAP65-1a. Likewise, transient expression of ZmMPK5 enhanced BR-induced activities of the antioxidant defence enzymes SOD and APX in a ZmMAP65- 1a-dependent manner. ZmMPK5 directly interacted with ZmMAP65-1a in vivo and phosphorylated ZmMAP65-1a in vitro. These results suggest that BR-induced antioxidant defence in maize operates through the interaction of ZmMPK5 with ZmMAP65-1a. Furthermore, ZmMAP65-1a functions in H2O2 self-propagation via regulation of the expression of NADPH oxidase genes in BR signalling.
authors: College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, PR China.
authors: Yuan
authors: Y
authors: Zhu
authors: Mingxing
authors: M
authors: Zuo
authors: Yali
authors: Y
authors: Liang
authors: Mingyi
authors: M
authors: Jiang
authors: Jianhua
authors: J
authors: Zhang
authors: Henrik Vibe
authors: HV
authors: Scheller
authors: Mingpu
authors: M
authors: Tan
authors: Aying
authors: A
authors: Zhang
mesh_terms: D000975:Antioxidants / Q000378:metabolism*; D060406:Brassinosteroids / Q000378:metabolism*; D006861:Hydrogen Peroxide / Q000378:metabolism; D020928:Mitogen-Activated Protein Kinases / Q000235:genetics / Q000378:metabolism*; D018515:Plant Leaves / Q000201:enzymology / Q000235:genetics; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D016133:Polymerase Chain Reaction; D011523:Protoplasts / Q000201:enzymology; D015398:Signal Transduction; D003313:Zea mays / Q000201:enzymology / Q000235:genetics*
chemical_list: D000975:Antioxidants; D060406:Brassinosteroids; D010940:Plant Proteins; D006861:Hydrogen Peroxide; D020928:Mitogen-Activated Protein Kinases; C402117:ZmMPK5
keywords: Antioxidant defence; H2O2; NADPH oxidase; ZmMAP65-1a; ZmMPK5.; brassinosteroid
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