title: Epithelial urinary bladder tumors from cows with enzootic hematuria: structural and cell cycle-related protein expression.
abstract: Thirty-nine epithelial bladder tumor samples from 37 animals affected with bovine enzootic hematuria (BEH) were selected for immunohistochemical studies. The expression of structural proteins such as uroplakin III (UPIII) and cytokeratin 7 (CK7) and the cell cycle-related proteins cyclin D1 and p53 were evaluated in urothelial papillomas and carcinomas. Loss of UPIII and CK7 expression was seen in both high-grade and high-stage urothelial carcinomas (P < .001 and P < .02). Cyclin D1 expression showed no statistically significant association with grade or stage. In contrast, p53 immunoreactivity was positive in high-grade and high-stage carcinomas (P < .05 and P < .01), confirming its association with the highest malignant behavior of the bladder tumors in BEH.
authors: Faculdade de Medicina Veterinária, CIISA, Universidade Técnica de Lisboa, Lisboa, Portugal adjoaobcota@fmv.utl.pt.
authors: J B
authors: JB
authors: Cota
authors: Instituto de Medicina Molecular, Lisboa, Portugal.
authors: T
authors: T
authors: Carvalho
authors: Serviço de Desenvolvimento Agrário de São Miguel, Quinta de S Gonçalo, Ponta Delgada, Portugal.
authors: C
authors: C
authors: Pinto
authors: Faculdade de Medicina Veterinária, CIISA, Universidade Técnica de Lisboa, Lisboa, Portugal.
authors: M C
authors: MC
authors: Peleteiro
mesh_terms: D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000378:metabolism* / Q000473:pathology* / Q000652:urine; D019938:Cyclin D1 / Q000378:metabolism; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000502:physiology*; D006417:Hematuria / Q000209:etiology / Q000662:veterinary*; D007150:Immunohistochemistry / Q000662:veterinary; D053552:Keratin-7 / Q000378:metabolism; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism; D001749:Urinary Bladder Neoplasms / Q000150:complications / Q000378:metabolism / Q000473:pathology / Q000662:veterinary*; D060171:Uroplakin III / Q000378:metabolism
chemical_list: D053552:Keratin-7; D016159:Tumor Suppressor Protein p53; D060171:Uroplakin III; D019938:Cyclin D1
keywords: CK7; bovine enzootic hematuria; cyclin D1; immunohistochemistry; p53; urinary bladder cancer; uroplakin III; urothelial tumors