title: Gag-dependent enrichment of HIV-1 RNA near the uropod membrane of polarized T cells.
abstract: The enrichment of HIV-1 macromolecules at the uropod of polarized T cells can significantly promote virus assembly and cell-mediated infection. Using live-cell fluorescence microscopy, we demonstrate that full-length HIV-1 RNA is enriched at the uropod membrane; furthermore, the presence of HIV-1 Gag containing a functional nucleocapsid domain is necessary for this HIV-1 RNA enrichment. The results from these studies provide novel insights into the mechanism of HIV-1 replication in polarized T cells.
authors: Viral Recombination Section.
authors: Steven C
authors: SC
authors: Hatch
authors: Luca
authors: L
authors: Sardo
authors: Jianbo
authors: J
authors: Chen
authors: Ryan
authors: R
authors: Burdick
authors: Robert
authors: R
authors: Gorelick
authors: Matthew J
authors: MJ
authors: Fivash
authors: Vinay K
authors: VK
authors: Pathak
authors: Wei-Shau
authors: WS
authors: Hu
mesh_terms: D015496:CD4-Positive T-Lymphocytes / Q000821:virology*; D002460:Cell Line; D002462:Cell Membrane / Q000821:virology*; D015497:HIV-1 / Q000502:physiology*; D006801:Humans; D046911:Macromolecular Substances / Q000378:metabolism*; D008856:Microscopy, Fluorescence; D012367:RNA, Viral / Q000378:metabolism*; D019065:Virus Assembly; D054301:gag Gene Products, Human Immunodeficiency Virus / Q000378:metabolism*
chemical_list: D046911:Macromolecular Substances; D012367:RNA, Viral; D054301:gag Gene Products, Human Immunodeficiency Virus
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