title: Biodegradation of malachite green by Ochrobactrum sp.
abstract: This study presents the biodegradation of malachite green (MG), a triphenylmethane dye, using a novel microorganism isolated from textile effluent contaminated environment. The organism responsible for degradation was identified as Ochrobactrum sp JN214485 by 16S rRNA analysis. The effect of operating parameters such as temperature, pH, immobilized bead loading, and initial dye concentration on % degradation was studied, and their optimal values were found to be 30 °C, 6, 20 g/L and 100 mg/L, respectively. The analysis showed that the extracellular enzymes were responsible for the degradation. The biodegradation of MG was confirmed by UV-visible spectroscopic and FTIR analysis. The phytotoxicity test concluded that the degradation products were less toxic compared to MG. The kinetics of biodegradation was studied and the activation energy was found to be 10.65 kcal/mol.
authors: Department of Chemical Engineering, Alagappa College of Technology, Anna University, Chennai, 600 025, India.
authors: S R
authors: SR
authors: Vijayalakshmidevi
authors: Karuppan
authors: K
authors: Muthukumar
mesh_terms: D001711:Biotransformation; D016000:Cluster Analysis; D004396:Coloring Agents / Q000378:metabolism*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D004783:Environmental Microbiology; D004798:Enzymes / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D042041:Ochrobactrum / Q000302:isolation & purification / Q000378:metabolism*; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012394:Rosaniline Dyes / Q000378:metabolism*; D017422:Sequence Analysis, DNA; D013053:Spectrophotometry; D017550:Spectroscopy, Fourier Transform Infrared; D013696:Temperature
chemical_list: D004396:Coloring Agents; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D004798:Enzymes; D012336:RNA, Ribosomal, 16S; D012394:Rosaniline Dyes; C005095:malachite green
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