title: Cohesin loading factor Nipbl localizes to chromosome axes during mammalian meiotic prophase.
abstract: BACKGROUND
  Sister chromatid cohesion mediated by the cohesin complex is essential for accurate chromosome segregation during mitosis and meiosis. Loading of cohesin onto chromosomes is dependent on another protein complex called kollerin, containing Nipbl/Scc2 and Mau2/Scc4. Nipbl is an evolutionarily conserved large protein whose haploinsufficiency in humans causes a developmental disorder called Cornelia de Lange syndrome. Although the function of Nipbl homologues for chromosome cohesion in meiotic cells of non-vertebrate models has been elucidated, Nipbl has not been characterized so far in mammalian spermatocytes or oocytes.
  
  
  RESULTS
  Here we describe our analyses on the expression and localization of Nipbl in nuclei of mouse spermatocytes and oocytes at different stages of meiotic prophase. In both spermatocytes and oocytes we found that Nipbl is associated with the axial/lateral element of the synaptonemal complex (AE/LE) to which cohesin also localizes. Interestingly, Nipbl in spermatocytes, but not in oocytes, dissociates from the AE/LE at mid-pachytene stage coincident with completion of DNA double-strand break repair.
  
  
  CONCLUSIONS
  Our data propose that cohesin loading activity is maintained during early stages of meiotic prophase in mammalian spermatocytes and oocytes.
authors: IRDB, Department of Surgery and Cancer, Imperial College London, Hammersmith Hospital, London W12 0NN, UK. n.kudo@imperial.ac.uk.
authors: Katarzyna
authors: K
authors: Kuleszewicz
authors: Xiangwei
authors: X
authors: Fu
authors: Nobuaki R
authors: NR
authors: Kudo
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