title: Therapeutic potential of biodegradable microparticles containing Punica granatum L. (pomegranate) in murine model of asthma.
abstract: OBJECTIVE
  Among the options for treatment of diseases affecting the respiratory system, especially asthma, drug delivering systems for intranasal application represent an important therapeutic approach at the site of inflammation. The present study aimed to evaluate the therapeutic effect of biodegradable microparticles formed by poly lactic-co-glycolic acid (PLGA) containing encapsulated pomegranate extract on a murine model of asthma.
  
  
  METHODS
  The extract was acquired from the leaves of P. granatum and characterized qualitatively by HPLC. A w/o/w emulsion solvent extraction-evaporation method was chosen to prepare the microparticles containing pomegranate encapsulated extract (MP).
  
  
  METHODS
  OVA-sensitized BALB/c mice were used as asthma model and treated with dexamethasone and P. granatum extract in solution form or encapsulated into microparticles.
  
  
  RESULTS
  MP were able to inhibit leukocytes' recruitment to bronchoalveolar fluid, especially, eosinophils, decreasing cytokines (IL-1β and IL-5) and protein levels in the lungs.
  
  
  CONCLUSIONS
  This approach can be used as an alternative/supplementary therapy based on the biological effects of P. granatum for managing inflammatory processes, especially those with pulmonary complications.
authors: Pró-Reitoria de Pós-Graduação, Pesquisa e Extensão, Universidade CEUMA (UNICEUMA), Rua Josué Montello, 1, São Luís, Maranhão, 65075-120, Brazil.
authors: Jéssica F F
authors: JF
authors: de Oliveira
authors: Diego V
authors: DV
authors: Garreto
authors: Mayara C P
authors: MC
authors: da Silva
authors: Thiare S
authors: TS
authors: Fortes
authors: Rejane B
authors: RB
authors: de Oliveira
authors: Flávia R F
authors: FR
authors: Nascimento
authors: Fernando B
authors: FB
authors: Da Costa
authors: Marcos A G
authors: MA
authors: Grisotto
authors: Roberto
authors: R
authors: Nicolete
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage* / Q000737:chemistry; D001249:Asthma / Q000188:drug therapy* / Q000276:immunology / Q000473:pathology; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology / Q000276:immunology; D016207:Cytokines / Q000276:immunology; D004195:Disease Models, Animal; D004337:Drug Carriers / Q000008:administration & dosage* / Q000737:chemistry; D005260:Female; D019344:Lactic Acid / Q000737:chemistry; D007962:Leukocytes / Q000187:drug effects / Q000276:immunology; D008168:Lung / Q000187:drug effects / Q000276:immunology / Q000473:pathology; D029561:Lythraceae; D051379:Mice; D008807:Mice, Inbred BALB C; D008517:Phytotherapy; D010936:Plant Extracts / Q000008:administration & dosage* / Q000737:chemistry; D018515:Plant Leaves; D011100:Polyglycolic Acid / Q000737:chemistry; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer
chemical_list: D000893:Anti-Inflammatory Agents; D016207:Cytokines; D004337:Drug Carriers; D010936:Plant Extracts; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid
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