title: The heme a synthase Cox15 associates with cytochrome c oxidase assembly intermediates during Cox1 maturation.
abstract: Cox1, the core subunit of the cytochrome c oxidase, receives two heme a cofactors during assembly of the 13-subunit enzyme complex. However, at which step of the assembly process and how heme is inserted into Cox1 have remained an enigma. Shy1, the yeast SURF1 homolog, has been implicated in heme transfer to Cox1, whereas the heme a synthase, Cox15, catalyzes the final step of heme a synthesis. Here we performed a comprehensive analysis of cytochrome c oxidase assembly intermediates containing Shy1. Our analyses suggest that Cox15 displays a role in cytochrome c oxidase assembly, which is independent of its functions as the heme a synthase. Cox15 forms protein complexes with Shy1 and also associates with Cox1-containing complexes independently of Shy1 function. These findings indicate that Shy1 does not serve as a mobile heme carrier between the heme a synthase and maturing Cox1 but rather cooperates with Cox15 for heme transfer and insertion in early assembly intermediates of cytochrome c oxidase.
authors: Department of Cellular Biochemistry, University Medical Center Göttingen, Göttingen, Germany.
authors: Bettina
authors: B
authors: Bareth
authors: Sven
authors: S
authors: Dennerlein
authors: David U
authors: DU
authors: Mick
authors: Miroslav
authors: M
authors: Nikolov
authors: Henning
authors: H
authors: Urlaub
authors: Peter
authors: P
authors: Rehling
mesh_terms: D001665:Binding Sites; D003576:Electron Transport Complex IV / Q000235:genetics* / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal; D006418:Heme / Q000378:metabolism; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D024101:Mitochondrial Proteins / Q000235:genetics* / Q000378:metabolism; D011485:Protein Binding; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics* / Q000378:metabolism; D015398:Signal Transduction
chemical_list: C107633:COX15 protein, S cerevisiae; D008565:Membrane Proteins; D024101:Mitochondrial Proteins; C106291:SHY1 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D006418:Heme; C526453:Cox1 protein, S cerevisiae; D003576:Electron Transport Complex IV
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