title: Activation of JNKs is essential for BMP9-induced osteogenic differentiation of mesenchymal stem cells.
abstract: Although BMP9 is highly capable of promoting osteogenic differentiation of mesenchymal stem cell (MSCs), the molecular mechanism involved remains to be fully elucidated. Here, we explore the possible involvement and detail role of JNKs (c-Jun N-terminal kinases) in BMP9-induced osteogenic differentiation of MSCs. It was found that BMP9 stimulated the activation of JNKs in MSCs. BMP9-induced osteogenic differentiation of MSCs was dramatically inhibited by JNKs inhibitor SP600125. Moreover, BMP9-activated Smads signaling was decreased by SP600125 treatment in MSCs. The effects of inhibitor are reproduced with adenoviruses expressing siRNA targeted JNKs. Taken together, our results revealed that JNKs was activated in BMP9-induced osteogenic differentiation of MSCs. What is most noteworthy, however, is that inhibition of JNKs activity resulted in reduction of BMP9-induced osteogenic differentiation of MSCs, implying that activation of JNKs is essential for BMP9 osteoinductive activity.
authors: Key Laboratory of Diagnostic Medicine Designated by the Chinese Ministry of Education, Chongqing Medical University, Chongqing, PR China.
authors: Yan-fang
authors: YF
authors: Zhao
authors: Jing
authors: J
authors: Xu
authors: Wen-juan
authors: WJ
authors: Wang
authors: Jin
authors: J
authors: Wang
authors: Juan-wen
authors: JW
authors: He
authors: Li
authors: L
authors: Li
authors: Qian
authors: Q
authors: Dong
authors: Yan
authors: Y
authors: Xiao
authors: Xing-lian
authors: XL
authors: Duan
authors: Xue
authors: X
authors: Yang
authors: Yi-wen
authors: YW
authors: Liang
authors: Tao
authors: T
authors: Song
authors: Min
authors: M
authors: Tang
authors: Dan
authors: D
authors: Zhao
authors: Jin-yong
authors: JY
authors: Luo
mesh_terms: D000818:Animals; D000873:Anthracenes / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects*; D002478:Cells, Cultured; D055412:Growth Differentiation Factors / Q000494:pharmacology*; D045325:HCT116 Cells; D006801:Humans; D048031:JNK Mitogen-Activated Protein Kinases / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D051379:Mice; D010012:Osteogenesis / Q000187:drug effects*; D010766:Phosphorylation / Q000187:drug effects; D034622:RNA Interference; D034741:RNA, Small Interfering; D015398:Signal Transduction / Q000187:drug effects; D051785:Smad Proteins / Q000378:metabolism
chemical_list: D000873:Anthracenes; D055412:Growth Differentiation Factors; D034741:RNA, Small Interfering; D051785:Smad Proteins; C432165:pyrazolanthrone; D048031:JNK Mitogen-Activated Protein Kinases
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