title: Development of bioactive porous α-TCP/HAp beads for bone tissue engineering.
abstract: Porous beads of bioactive ceramics such as hydroxyapatite (HAp) and tribasic calcium phosphate (TCP) are considered a promising scaffold for cultivating bone cells. To realize this, α-TCP/HAp functionally graded porous beads are fabricated with two main purposes: to maintain the function of the scaffold with sufficient strength up to the growth of new bone, and is absorbed completely after the growth. HAp is a bioactive material that has both high strength and strong tissue-adhesive properties, but is not readily absorbed by the human body. On the contrary, α-TCP is highly bioabsorbable, resulting in a scaffold that is absorbed before it is completely replaced by bone. In this study, we produced porous, bead-shaped carriers as scaffolds for osteoblast culture. To control the solubility in vivo, the fabricated beads contained α-TCP at the center and HAp at the surface. Cell adaptability of these beads for bone tissue engineering was confirmed in vitro. It was found that α-TCP/HAp bead carriers exhibit low toxicity in the initial stages of cell seeding and cell adhesion. The presence of HAp in the composite bead form effectively increased ALP activity. In conclusion, it is suggested that these newly developed α-TCP/HAp beads are a promising tool for bone tissue engineering.
authors: Division of Mechanical Design Engineering, Graduate School of Science and Engineering, Tokyo Denki University, Hatoyama, Hiki-gun, Saitama, 350-0394, Japan.
authors: Teruo
authors: T
authors: Asaoka
authors: Shoji
authors: S
authors: Ohtake
authors: Katsuko S
authors: KS
authors: Furukawa
authors: Akito
authors: A
authors: Tamura
authors: Takashi
authors: T
authors: Ushida
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology*; D001842:Bone and Bones / Q000187:drug effects* / Q000502:physiology*; D002130:Calcium Phosphates / Q000494:pharmacology*; D002452:Cell Count; D002460:Cell Line; D017886:Durapatite / Q000494:pharmacology*; D006801:Humans; D007211:Indoles / Q000378:metabolism; D051379:Mice; D008855:Microscopy, Electron, Scanning; D008856:Microscopy, Fluorescence; D008863:Microspheres; D009930:Organic Chemicals / Q000378:metabolism; D010012:Osteogenesis / Q000187:drug effects; D016062:Porosity; D023822:Tissue Engineering / Q000379:methods*; D014961:X-Ray Diffraction
chemical_list: D001672:Biocompatible Materials; D002130:Calcium Phosphates; D007211:Indoles; D009930:Organic Chemicals; C099476:PicoGreen; C007293:DAPI; D017886:Durapatite; D000469:Alkaline Phosphatase; C018392:tricalcium phosphate
keywords: HAp; TCP; bone tissue engineering; functionally graded material; porous beads; scaffold