title: Isolation and mutational analysis of circulating tumor cells from lung cancer patients with magnetic sifters and biochips.
abstract: Detection and characterization of circulating tumor cells (CTCs) may reveal insights into the diagnosis and treatment of malignant disease. Technologies for isolating CTCs developed thus far suffer from one or more limitations, such as low throughput, inability to release captured cells, and reliance on expensive instrumentation for enrichment or subsequent characterization. We report a continuing development of a magnetic separation device, the magnetic sifter, which is a miniature microfluidic chip with a dense array of magnetic pores. It offers high efficiency capture of tumor cells, labeled with magnetic nanoparticles, from whole blood with high throughput and efficient release of captured cells. For subsequent characterization of CTCs, an assay, using a protein chip with giant magnetoresistive nanosensors, has been implemented for mutational analysis of CTCs enriched with the magnetic sifter. The use of these magnetic technologies, which are separate devices, may lead the way to routine preparation and characterization of "liquid biopsies" from cancer patients.
authors: Department of Materials Science and Engineering, Stanford University, Stanford, CA 94305, USA.
authors: Christopher M
authors: CM
authors: Earhart
authors: Casey E
authors: CE
authors: Hughes
authors: Richard S
authors: RS
authors: Gaster
authors: Chin Chun
authors: CC
authors: Ooi
authors: Robert J
authors: RJ
authors: Wilson
authors: Lisa Y
authors: LY
authors: Zhou
authors: Eric W
authors: EW
authors: Humke
authors: Lingyun
authors: L
authors: Xu
authors: Dawson J
authors: DJ
authors: Wong
authors: Stephen B
authors: SB
authors: Willingham
authors: Erich J
authors: EJ
authors: Schwartz
authors: Irving L
authors: IL
authors: Weissman
authors: Stefanie S
authors: SS
authors: Jeffrey
authors: Joel W
authors: JW
authors: Neal
authors: Rajat
authors: R
authors: Rohatgi
authors: Heather A
authors: HA
authors: Wakelee
authors: Shan X
authors: SX
authors: Wang
mesh_terms: D055910:Antibodies, Immobilized / Q000737:chemistry / Q000276:immunology; D000951:Antigens, Neoplasm / Q000276:immunology / Q000378:metabolism; D002289:Carcinoma, Non-Small-Cell Lung / Q000378:metabolism / Q000473:pathology; D015815:Cell Adhesion Molecules / Q000276:immunology / Q000378:metabolism; D045744:Cell Line, Tumor; D002469:Cell Separation / Q000295:instrumentation / Q000379:methods*; D000071858:Epithelial Cell Adhesion Molecule; D066246:ErbB Receptors / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D016650:Fluorescein-5-isothiocyanate / Q000737:chemistry; D006801:Humans; D007633:Keratins / Q000276:immunology / Q000378:metabolism; D008175:Lung Neoplasms / Q000378:metabolism / Q000473:pathology; D061986:MCF-7 Cells; D008280:Magnetics; D058185:Magnetite Nanoparticles / Q000737:chemistry; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods*; D009154:Mutation; D009360:Neoplastic Cells, Circulating / Q000378:metabolism*
chemical_list: D055910:Antibodies, Immobilized; D000951:Antigens, Neoplasm; D015815:Cell Adhesion Molecules; D000071858:Epithelial Cell Adhesion Molecule; D058185:Magnetite Nanoparticles; D007633:Keratins; D066246:ErbB Receptors; D016650:Fluorescein-5-isothiocyanate
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