title: Galectin-1 induces 12/15-lipoxygenase expression in murine macrophages and favors their conversion toward a pro-resolving phenotype.
abstract: During the resolution of inflammation macrophages undergo functional changes upon exposure to pro-resolving agents in their microenvironment. Primarily, engulfment of apoptotic polymorphonuclear (PMN) cells promotes conversion of macrophages toward a pro-resolving phenotype characterized by reduced CD11b expression. These macrophages are not phagocytic, do not respond to TLR ligands, and express relatively high levels of the pro-resolving enzyme 12/15-lipoxygenase (LO). Here, we report that the immuno-regulatory lectin galectin-1 is selectively expressed by CD11b(high), but not CD11b(low) macrophages. Upon exposure in vivo and ex vivo, galectin-1 directly promoted macrophage conversion from a CD11b(high) to a CD11b(low) phenotype and up-regulated the expression and activity of 12/15-LO. Moreover, galectin-1 treatment in vivo promoted the loss of phagocytic capacity (efferocytic satiation) in peritoneal macrophages and down-regulated secretion of TNF-α, IL-1β, and IL-10 upon LPS exposure. Our results suggest that galectin-1 could be an essential mediator in the control of macrophage function during the resolution of inflammation.
authors: Department of Biology, Faculty of Natural Sciences, University of Haifa, Haifa 31905, Israel.
authors: Ran
authors: R
authors: Rostoker
authors: Hiba
authors: H
authors: Yaseen
authors: Sagie
authors: S
authors: Schif-Zuck
authors: Rachel G
authors: RG
authors: Lichtenstein
authors: Gabriel A
authors: GA
authors: Rabinovich
authors: Amiram
authors: A
authors: Ariel
mesh_terms: D000818:Animals; D017209:Apoptosis; D001092:Arachidonate 12-Lipoxygenase / Q000235:genetics / Q000378:metabolism*; D001093:Arachidonate 15-Lipoxygenase / Q000235:genetics / Q000378:metabolism*; D018845:CD11 Antigens / Q000378:metabolism; D002478:Cells, Cultured; D016207:Cytokines / Q000378:metabolism; D004790:Enzyme Induction; D037483:Galectin 1 / Q000502:physiology*; D007249:Inflammation / Q000201:enzymology; D008070:Lipopolysaccharides / Q000494:pharmacology; D017737:Macrophages, Peritoneal / Q000201:enzymology* / Q000276:immunology / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D010641:Phenotype
chemical_list: D018845:CD11 Antigens; D016207:Cytokines; D037483:Galectin 1; D008070:Lipopolysaccharides; D001092:Arachidonate 12-Lipoxygenase; D001093:Arachidonate 15-Lipoxygenase
keywords: 15-Lipoxygenase; Efferocytosis; Galectin-1; Macrophages; Resolution of inflammation