title: A comparative approach expands the protein-protein interaction node of the immune receptor XA21 in wheat and rice.
abstract: The rice (Oryza sativa) OsXA21 receptor kinase is a well-studied immune receptor that initiates a signal transduction pathway leading to resistance to Xanthomonas oryzae pv. oryzae. Two homologs of OsXA21 were identified in wheat (Triticum aestivum): TaXA21-like1 located in a syntenic region with OsXA21, and TaXA21-like2 located in a nonsyntenic region. Proteins encoded by these two wheat genes interact with four wheat orthologs of known OsXA21 interactors. In this study, we screened a wheat yeast-two-hybrid (Y2H) library using the cytosolic portion of TaXA21-like1 as bait to identify additional interactors. Using full-length T. aestivum and T. monococcum proteins and Y2H assays we identified three novel TaXA21-like1 interactors (TaARG, TaPR2, TmSKL1) plus one previously known in rice (TaSGT1). An additional full-length wheat protein (TaCIPK14) interacted with TaXA21-like2 and OsXA21 but not with TaXA21-like1. The interactions of TaXA21-like1 with TmSKL1 and TaSGT1 were also observed in rice protoplasts using bimolecular fluorescence complementation assays. We then cloned the rice homologs of the novel wheat interactors and confirmed that they all interact with OsXA21. This last result suggests that interspecific comparative interactome analyses can be used not only to transfer known interactions from rice to wheat, but also to identify novel interactions in rice.
authors: State Key Laboratory of Crop Stress Biology for Arid Areas/College of Agronomy, Northwest A&F University, 3 Taicheng Road, Yangling, Shaanxi 712100, China.
authors: Baoju
authors: B
authors: Yang
authors: Randy
authors: R
authors: Ruan
authors: Dario
authors: D
authors: Cantu
authors: Xiaodong
authors: X
authors: Wang
authors: Wanquan
authors: W
authors: Ji
authors: Pamela C
authors: PC
authors: Ronald
authors: Jorge
authors: J
authors: Dubcovsky
mesh_terms: D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D015723:Gene Library; D018841:HSP90 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D012275:Oryza / Q000235:genetics / Q000378:metabolism* / Q000382:microbiology; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism*; D060066:Protein Interaction Maps; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism*; D011523:Protoplasts / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D014908:Triticum / Q000235:genetics / Q000378:metabolism* / Q000382:microbiology; D020798:Two-Hybrid System Techniques
chemical_list: D018841:HSP90 Heat-Shock Proteins; D010940:Plant Proteins; C097148:Xa21 protein, Oryza sativa; D017346:Protein Serine-Threonine Kinases
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