title: Regulation of selenoprotein P concentration and expression by different sources of selenium in broiler breeders and their offspring.
abstract: An 8-wk experiment using 180 Lingnan Yellow broiler breeders was conducted to investigate the effect of different sources of Se on the concentration and gene expression of selenoprotein P (SEPP1) in broiler breeders and their offspring. After receiving a low-Se basal diet for 8 wk, broiler breeders were randomly allocated to 3 treatments (6 replicates per treatment) and fed corn-soy-based diets supplemented with 0.15 mg/kg of Se from sodium selenite (SS), Se-enriched yeast (SY), and selenomethionine (SM), respectively. Fertile eggs from different replicates were collected during the last week of the experiment and then incubated in different incubator trays in the same incubator. The concentrations and mRNA levels of SEPP1 were determined in liver and kidney of broiler breeders and their offspring, as well as the serum SEPP1 concentration. Compared with SS, both SY and SM significantly increased (P < 0.05) the concentration and mRNA level of SEPP1 in 1-d-old chicks. In broiler breeders, the serum and liver SEPP1 concentrations were notably increased by the use of organic Se (P < 0.05), whereas no differences were found in the kidney. Moreover, the SEPP1 mRNA level in the liver was remarkably elevated (P < 0.05) in organic Se treatments, but also no differences were observed in the kidney. Results of this study indicate that the use of organic Se (SY or SM) in maternal diets is very effective for increasing the concentration and mRNA level of SEPP1, especially in their offspring. No differences between SY and SM were observed.
authors: Feed Science Institute, College of Animal Science, Zhejiang University, No. 866, Yuhangtang Road, Hangzhou 310058, People's Republic of China.
authors: D
authors: D
authors: Yuan
authors: L
authors: L
authors: Zheng
authors: X Y
authors: XY
authors: Guo
authors: Y X
authors: YX
authors: Wang
authors: X A
authors: XA
authors: Zhan
mesh_terms: D000821:Animal Feed / Q000032:analysis; D000818:Animals; D002645:Chickens / Q000235:genetics / Q000254:growth & development / Q000502:physiology*; D019587:Dietary Supplements / Q000032:analysis*; D005260:Female; D005786:Gene Expression Regulation; D007668:Kidney / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis; D011897:Random Allocation; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary; D012645:Selenomethionine / Q000008:administration & dosage / Q000378:metabolism*; D051149:Selenoprotein P / Q000097:blood / Q000235:genetics* / Q000378:metabolism; D018038:Sodium Selenite / Q000008:administration & dosage / Q000378:metabolism*; D015002:Yeast, Dried / Q000008:administration & dosage / Q000737:chemistry
chemical_list: D012333:RNA, Messenger; D051149:Selenoprotein P; D012645:Selenomethionine; D018038:Sodium Selenite