title: Simple and rapid characterization of mycolic acids from Dietzia strains by using MALDI spiral-TOFMS with ultra high mass-resolving power.
abstract: Mycolic acids have been used as important chemotaxonomic markers. In this study, a newly developed matrix-assisted laser desorption/ionization time-of-flight mass spectrometer with a spiral ion trajectory (MALDI spiral-TOFMS) was applied to the characterization of mycolic acids of three type strains of validated species belonging to the genus Dietzia (D. papillomatosis 105045(T), D. kunjamensis NBRC 105042(T) and D. timorensis NBRC 104184(T)), by analysis of total fatty acid methyl ester fractions. In addition, owing to the high mass-resolving power of MALDI spiral-TOFMS, adjacent peaks (0.036 Da mass differences) were successfully separated, and weak peaks corresponding to oxygenated mycolic acids were detected. For all samples, the distributions of carbon-chain lengths were mainly in the range of C30-C42 and the average number of carbon-chain lengths was about 37, which agreed reasonably well with reported results for the genus Dietzia. The number of double bonds and/or cyclopropane rings was 0-2. Relative peak intensities of each mycolic acid methyl ester were used to compare the mycolic acids of the three strains. The mycolic acids of D. papillomatosis and D. kunjamensis were characterized by a high content of mycolic acids with 0-1 double bond or cyclopropane ring and an almost equal content of mycolic acids with odd- and even-numbered carbon-chain lengths. In contrast, mycolic acids of D. timorensis were characterized by a high content of mycolic acids with 1-2 double bonds and/or cyclopropane rings with an even-numbered carbon-chain length. By using MALDI spiral-TOFMS, mycolic acids from three type strains of the genus Dietzia were characterized easily and rapidly.
authors: Advanced Technology Department, JEOL Ltd., Akishima, Japan.
authors: Kanae
authors: K
authors: Teramoto
authors: Resource Collection Division, NITE Biological Resource Center Department of Biotechnology, Kisarazu, Japan.
authors: Tomohiko
authors: T
authors: Tamura
authors: Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Japan.
authors: Satoshi
authors: S
authors: Hanada
authors: MS Business Unit, JEOL Ltd., Akishima, Japan.
authors: Takafumi
authors: T
authors: Sato
authors: Resource Collection Division, NITE Biological Resource Center Department of Biotechnology, Kisarazu, Japan.
authors: Hiroko
authors: H
authors: Kawasaki
authors: Resource Collection Division, NITE Biological Resource Center Department of Biotechnology, Kisarazu, Japan.
authors: Ken-ichiro
authors: K
authors: Suzuki
authors: Research Institute for Environmental Management Technology, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Japan.
authors: Hiroaki
authors: H
authors: Sato
mesh_terms: D000192:Actinomycetales / Q000737:chemistry* / Q000145:classification*; D003521:Cyclopropanes / Q000737:chemistry; D015394:Molecular Structure; D008970:Molecular Weight; D009171:Mycolic Acids / Q000737:chemistry*; D013045:Species Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods
chemical_list: D003521:Cyclopropanes; D009171:Mycolic Acids