title: Lymphatic transport of puerarin occurs after oral administration of different lipid-based formulations to unconscious lymph duct-cannulated rats.
abstract: This study investigated the potential of targeted intestinal lymphatic transport of puerarin via a lipid formulation approach. Three formulations of PEG nanoemulsion, nanosuspension and an oil suspension containing puerarin were examined with the lymph-cannulated anaesthetized rat model. Plasma and lymph samples were analyzed by HPLC. Lymph triglyceride was measured using an enzymatic colorimetric technique. After 8 h, the total administered dose accumulated in the thoracic lymph duct was analyze. Nanoemulsion, nanosuspension and oil suspension was 0.065 ± 0.006%, 0.137 ± 0.018%, 0.021 ± 0.002% of the administered dose, respectively. In nanoemulsion, nanosuspension and oil suspension group, the systemic bioavailability of oral puerarin was 11%, 16% and 11% for lymph-cannulated rats, 41%, 67% and 18% for control rats. Absorption into the intestinal lymph should thus contribute to ∼30%, 51% and 7% of the systemic bioavailable puerarin. This data indicated that lipid-based nano drug formulation produced higher lymph concentrations of puerarin than oil suspension. The nanosuspension formulation may be considerable in terms of increased local concentrations in lymphoid tissue.
authors: Department of Pharmaceutics, Anhui University of Chinese Medicine , Anhui Province , China .
authors: An
authors: A
authors: Zhou
authors: Tao
authors: T
authors: Lu
authors: Lei
authors: L
authors: Wang
authors: Chuanhua
authors: C
authors: Lu
authors: Lina
authors: L
authors: Wang
authors: Maolin
authors: M
authors: Wan
authors: Hongfei
authors: H
authors: Wu
mesh_terms: D000284:Administration, Oral; D000818:Animals; D001682:Biological Availability; D001692:Biological Transport; D002626:Chemistry, Pharmaceutical / Q000379:methods; D004655:Emulsions / Q000737:chemistry / Q000378:metabolism; D007408:Intestinal Absorption; D007529:Isoflavones / Q000737:chemistry* / Q000378:metabolism*; D008055:Lipids / Q000737:chemistry*; D008208:Lymphatic System / Q000378:metabolism*; D008297:Male; D053758:Nanoparticles / Q000737:chemistry / Q000378:metabolism; D009821:Oils / Q000737:chemistry / Q000378:metabolism; D011092:Polyethylene Glycols / Q000737:chemistry / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013535:Suspensions / Q000737:chemistry / Q000378:metabolism; D014280:Triglycerides / Q000378:metabolism
chemical_list: D004655:Emulsions; D007529:Isoflavones; D008055:Lipids; D009821:Oils; D013535:Suspensions; D014280:Triglycerides; D011092:Polyethylene Glycols; C033607:puerarin