title: Metabolite profile analysis reveals functional effects of 28-day vitamin B-6 restriction on one-carbon metabolism and tryptophan catabolic pathways in healthy men and women.
abstract: Suboptimal vitamin B-6 status, as reflected by low plasma pyridoxal 5'-phosphate (PLP) concentration, is associated with increased risk of vascular disease. PLP plays many roles, including in one-carbon metabolism for the acquisition and transfer of carbon units and in the transsulfuration pathway. PLP also serves as a coenzyme in the catabolism of tryptophan. We hypothesize that the pattern of these metabolites can provide information reflecting the functional impact of marginal vitamin B-6 deficiency. We report here the concentration of major constituents of one-carbon metabolic processes and the tryptophan catabolic pathway in plasma from 23 healthy men and women before and after a 28-d controlled dietary vitamin B-6 restriction (<0.35 mg/d). liquid chromatography-tandem mass spectrometry analysis of the compounds relevant to one-carbon metabolism showed that vitamin B-6 restriction yielded increased cystathionine (53% pre- and 76% postprandial; P < 0.0001) and serine (12% preprandial; P < 0.05), and lower creatine (40% pre- and postprandial; P < 0.0001), creatinine (9% postprandial; P < 0.05), and dimethylglycine (16% postprandial; P < 0.05) relative to the vitamin B-6-adequate state. In the tryptophan pathway, vitamin B-6 restriction yielded lower kynurenic acid (22% pre- and 20% postprandial; P < 0.01) and higher 3-hydroxykynurenine (39% pre- and 34% postprandial; P < 0.01). Multivariate ANOVA analysis showed a significant global effect of vitamin B-6 restriction and multilevel partial least squares-discriminant analysis supported this conclusion. Thus, plasma concentrations of creatine, cystathionine, kynurenic acid, and 3-hydroxykynurenine jointly reveal effects of vitamin B-6 restriction on the profiles of one-carbon and tryptophan metabolites and serve as biomarkers of functional effects of marginal vitamin B-6 deficiency.
authors: Food Science and Human Nutrition Department, Institute of Food and Agricultural Sciences.
authors: Vanessa R
authors: VR
authors: da Silva
authors: Luisa
authors: L
authors: Rios-Avila
authors: Yvonne
authors: Y
authors: Lamers
authors: Maria A
authors: MA
authors: Ralat
authors: Øivind
authors: Ø
authors: Midttun
authors: Eoin P
authors: EP
authors: Quinlivan
authors: Timothy J
authors: TJ
authors: Garrett
authors: Bonnie
authors: B
authors: Coats
authors: Meena N
authors: MN
authors: Shankar
authors: Susan S
authors: SS
authors: Percival
authors: Yueh-Yun
authors: YY
authors: Chi
authors: Keith E
authors: KE
authors: Muller
authors: Per Magne
authors: PM
authors: Ueland
authors: Peter W
authors: PW
authors: Stacpoole
authors: Jesse F
authors: JF
authors: Gregory
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000097:blood; D003401:Creatine / Q000097:blood; D003540:Cystathionine / Q000097:blood; D005260:Female; D006801:Humans; D007249:Inflammation / Q000097:blood; D007736:Kynurenic Acid / Q000097:blood; D007737:Kynurenine / Q000031:analogs & derivatives / Q000097:blood; D008297:Male; D015999:Multivariate Analysis; D019518:Postprandial Period; D011732:Pyridoxal Phosphate / Q000097:blood; D012694:Serine / Q000097:blood; D014364:Tryptophan / Q000378:metabolism*; D025101:Vitamin B 6 / Q000008:administration & dosage / Q000097:blood*; D026681:Vitamin B 6 Deficiency / Q000097:blood*; D055815:Young Adult
chemical_list: D015415:Biomarkers; C005045:3-hydroxykynurenine; D007737:Kynurenine; D003540:Cystathionine; D012694:Serine; D011732:Pyridoxal Phosphate; D025101:Vitamin B 6; D014364:Tryptophan; D007736:Kynurenic Acid; D003401:Creatine
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