title: High throughput detection of human neutrophil peptides from serum, saliva, and tear by anthrax lethal factor-modified nanoparticles.
abstract: Human α defensins human neutrophil peptide 1-3 (HNP 1-3) are potential prognostic cancer biomarkers. Metalloprotein anthrax lethal factor (ALF) binds to HNP 1-3 in a Zn2+-dependent manner. We conjugated ALF to the surface of magnetic nanoparticles (MNP) to magnetically isolate the HNPs, and used Zn2+ to control the capture and release HNPs.
authors: CAS Key Laboratory for Biological Effects of Nanomaterials & Nanosafety, National Center for Nanoscience and Technology , 11 Beiyitiao, Zhongguancun, Beijing 100190, China.
authors: Yuanyuan
authors: Y
authors: Zhao
authors: Xu
authors: X
authors: Zhang
authors: Xiangdong
authors: X
authors: Xue
authors: Zhipeng
authors: Z
authors: Li
authors: Fei
authors: F
authors: Chen
authors: Shengliang
authors: S
authors: Li
authors: Anil
authors: A
authors: Kumar
authors: Guozhang
authors: G
authors: Zou
authors: Xing-Jie
authors: XJ
authors: Liang
mesh_terms: D000942:Antigens, Bacterial / Q000737:chemistry* / Q000378:metabolism; D001427:Bacterial Toxins / Q000737:chemistry* / Q000378:metabolism; D015415:Biomarkers / Q000032:analysis / Q000097:blood; D002851:Chromatography, High Pressure Liquid; D052203:Ferrosoferric Oxide / Q000737:chemistry; D006801:Humans; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D012463:Saliva / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013666:Tears / Q000378:metabolism*; D015032:Zinc / Q000737:chemistry; D023084:alpha-Defensins / Q000032:analysis* / Q000097:blood
chemical_list: D000942:Antigens, Bacterial; D001427:Bacterial Toxins; D015415:Biomarkers; D058185:Magnetite Nanoparticles; D023084:alpha-Defensins; C030325:anthrax toxin; C406413:human neutrophil peptide 1; D015032:Zinc; D052203:Ferrosoferric Oxide