title: Identification of neoblast- and regeneration-specific miRNAs in the planarian Schmidtea mediterranea.
abstract: In recent years, the planarian Schmidtea mediterranea has emerged as a tractable model system to study stem cell biology and regeneration. MicroRNAs are small RNA species that control gene expression by modulating translational repression and mRNA stability and have been implicated in the regulation of various cellular processes. Though recent studies have identified several miRNAs in S. mediterranea, their expression in neoblast subpopulations and during regeneration has not been examined. Here, we identify several miRNAs whose expression is enriched in different neoblast subpopulations and in regenerating tissue at different time points in S. mediterranea. Some of these miRNAs were enriched within 3 h post-amputation and may, therefore, play a role in wound healing and/or neoblast migration. Our results also revealed miRNAs, such as sme-miR-2d-3p and the sme-miR-124 family, whose expression is enriched in the cephalic ganglia, are also expressed in the brain primordium during CNS regeneration. These results provide new insight into the potential biological functions of miRNAs in neoblasts and regeneration in planarians.
authors: Vidyanand
authors: V
authors: Sasidharan
authors: Yi-Chien
authors: YC
authors: Lu
authors: Dhiru
authors: D
authors: Bansal
authors: Pranavi
authors: P
authors: Dasari
authors: Deepak
authors: D
authors: Poduval
authors: Aswin
authors: A
authors: Seshasayee
authors: Alissa M
authors: AM
authors: Resch
authors: Brenton R
authors: BR
authors: Graveley
authors: Dasaradhi
authors: D
authors: Palakodeti
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D019295:Computational Biology; D020869:Gene Expression Profiling; D017403:In Situ Hybridization; D035683:MicroRNAs / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis; D010932:Planarians / Q000166:cytology / Q000502:physiology*; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D012038:Regeneration / Q000502:physiology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013234:Stem Cells / Q000166:cytology / Q000502:physiology*
chemical_list: D015415:Biomarkers; D035683:MicroRNAs; D012333:RNA, Messenger
keywords: microRNA; neoblast; planaria; regeneration
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