title: Molecular characterization and phylogenetic analysis of new variants of the porcine epidemic diarrhea virus in Gansu, China in 2012.
abstract: Between January 2012 and March 2012, the infection rates of porcine epidemic diarrhea virus (PEDV) increased substantially in vaccinated swine herds in many porcine farms in Gansu Province, China. The spike (S) glycoprotein is an important determinant for PEDV biological properties. To determine the distribution profile of PEDV outbreak strains, we sequenced the full-length S gene of five samples from two farms where animals exhibited severe diarrhea and high mortality rates. Five new PEDV variants were identified, and the molecular diversity, phylogenetic relationships, and antigenicity analysis of Gansu field samples with other PEDV reference strains were investigated. A series of insertions, deletions, and mutations in the S gene was found in five PEDV variants compared with classical and vaccine strains. These mutations may provide stronger pathogenicity and antigenicity to the new PEDV variants that influenced the effectiveness of the CV777-based vaccine. Our results suggest that these new PEDV variant strains in Gansu Province might be from South Korean or South China, and the effectiveness of the CV777-based vaccine needs to be evaluated.
authors: College of Animal Science and Veterinary Medicine, Jilin University, Changchun 130062, China.
authors: Yufei
authors: Y
authors: Tian
authors: Zhijun
authors: Z
authors: Yu
authors: Kaihui
authors: K
authors: Cheng
authors: Yuxiu
authors: Y
authors: Liu
authors: Jing
authors: J
authors: Huang
authors: Yue
authors: Y
authors: Xin
authors: Yuanguo
authors: Y
authors: Li
authors: Shengtao
authors: S
authors: Fan
authors: Tiecheng
authors: T
authors: Wang
authors: Geng
authors: G
authors: Huang
authors: Na
authors: N
authors: Feng
authors: Zhenguo
authors: Z
authors: Yang
authors: Songtao
authors: S
authors: Yang
authors: Yuwei
authors: Y
authors: Gao
authors: Xianzhu
authors: X
authors: Xia
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000276:immunology; D002681:China / Q000453:epidemiology; D016000:Cluster Analysis; D018352:Coronavirus Infections / Q000453:epidemiology / Q000401:mortality / Q000662:veterinary* / Q000821:virology; D004196:Disease Outbreaks; D014644:Genetic Variation; D005838:Genotype; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D009154:Mutation; D010802:Phylogeny; D053485:Porcine epidemic diarrhea virus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D012367:RNA, Viral / Q000235:genetics*; D017422:Sequence Analysis, DNA; D064370:Spike Glycoprotein, Coronavirus / Q000235:genetics; D013552:Swine; D013553:Swine Diseases / Q000453:epidemiology* / Q000821:virology*; D014765:Viral Vaccines / Q000276:immunology
chemical_list: D000956:Antigens, Viral; D012367:RNA, Viral; D064370:Spike Glycoprotein, Coronavirus; D014765:Viral Vaccines
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