title: Production of diabetic offspring using cryopreserved epididymal sperm by in vitro fertilization and intrafallopian insemination techniques in transgenic pigs.
abstract: Somatic cell nuclear transfer (SCNT) is a useful technique for creating pig strains that model human diseases. However, production of numerous cloned disease model pigs by SCNT for large-scale experiments is impractical due to its complexity and inefficiency. In the present study, we aimed to establish an efficient procedure for proliferating the diabetes model pig carrying the mutant human hepatocyte nuclear factor-1α gene. A founder diabetes transgenic cloned pig was generated by SCNT and treated with insulin to allow for normal growth to maturity, at which point epididymal sperm could be collected for cryopreservation. In vitro fertilization and intrafallopian insemination using the cryopreserved epididymal sperm resulted in diabetes model transgenic offspring. These results suggest that artificial reproductive technology using cryopreserved epididymal sperm could be a practical option for proliferation of genetically modified disease model pigs.
authors: Laboratory of Developmental Engineering, Department of Life Sciences, School of Agriculture, Meiji University, Kanagawa 214-8571, Japan.
authors: Kazuhiro
authors: K
authors: Umeyama
authors: Kasumi
authors: K
authors: Honda
authors: Hitomi
authors: H
authors: Matsunari
authors: Kazuaki
authors: K
authors: Nakano
authors: Tatsuro
authors: T
authors: Hidaka
authors: Keito
authors: K
authors: Sekiguchi
authors: Hironori
authors: H
authors: Mochizuki
authors: Yasuhiro
authors: Y
authors: Takeuchi
authors: Tsukasa
authors: T
authors: Fujiwara
authors: Masahito
authors: M
authors: Watanabe
authors: Masaki
authors: M
authors: Nagaya
authors: Hiroshi
authors: H
authors: Nagashima
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D019976:Cloning, Organism / Q000662:veterinary; D015925:Cryopreservation / Q000662:veterinary*; D003920:Diabetes Mellitus / Q000188:drug therapy / Q000235:genetics / Q000662:veterinary*; D004195:Disease Models, Animal; D005260:Female; D005307:Fertilization in Vitro / Q000662:veterinary*; D051538:Hepatocyte Nuclear Factor 1-alpha / Q000235:genetics / Q000378:metabolism; D006801:Humans; D007004:Hypoglycemic Agents / Q000627:therapeutic use; D007315:Insemination, Artificial / Q000662:veterinary*; D007564:Japan; D008297:Male; D009154:Mutation; D053652:Nuclear Transfer Techniques / Q000662:veterinary; D011247:Pregnancy; D013094:Spermatozoa; D034421:Sus scrofa; D013552:Swine; D013553:Swine Diseases / Q000188:drug therapy / Q000235:genetics*
chemical_list: C496487:HNF1A protein, human; D051538:Hepatocyte Nuclear Factor 1-alpha; D007004:Hypoglycemic Agents
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