title: Conditional and unconditional QTL mapping of drought-tolerance-related traits of wheat seedling using two related RIL populations.
abstract: For discovering the quantitative trait loci (QTLs) contributing to early seedling growth and drought tolerance during germination, conditional and unconditional analyses of 12 traits of wheat seedlings: coleoptile length, seedling height, longest root length, root number, seedling fresh weight, stem and leaves fresh weight, root fresh weight, seedling dry weight, stem and leaves dry weight, root dry weight, root to shoot fresh weight ratio, root-to-shoot dry weight ratio, were conducted under two water conditions using two F8:9 recombinant inbred line (RIL) populations. The results of unconditional analysis are as follows: 88 QTLs accounting for 3.33-77.01% of the phenotypic variations were detected on chromosomes 1A, 1B, 1D, 2A, 2B, 2D, 3A, 3B, 4A, 4B, 4D, 5A, 5B, 5D, 6A, 6B, 6D, 7A, 7B and 7D. Among these QTLs, 19 were main-effect QTLs with a contribution rate greater than 10%. The results of the conditional QTL analysis of 12 traits under osmotic stress on normal water conditions were as follows: altogether 22 QTLs concerned with drought tolerance were detected on chromosomes 1B, 2A, 2B, 3B, 4A, 5D, 6A, 6D, 7B, and 7D. Of these QTLs, six were main-effect QTLs. These 22 QTLs were all special loci directly concerned with drought tolerance and most of them could not be detected by unconditional analysis. The finding of these QTLs has an important significance for fine-mapping technique, map-based cloning, and molecular marker-assisted selection of early seedling traits, such as growth and drought tolerance.
authors: State Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, Taian Subcenter of National Wheat Improvement Center, College of Agronomy, Shandong Agricultural University, Taian 271018, People's Republic of China.
authors: Hong
authors: H
authors: Zhang
authors: Fa
authors: F
authors: Cui
authors: Lin
authors: L
authors: Wang
authors: Jun
authors: J
authors: Li
authors: Anming
authors: A
authors: Ding
authors: Chunhua
authors: C
authors: Zhao
authors: Yinguang
authors: Y
authors: Bao
authors: Qiuping
authors: Q
authors: Yang
authors: Honggang
authors: H
authors: Wang
mesh_terms: D000220:Adaptation, Biological / Q000235:genetics*; D002874:Chromosome Mapping; D032461:Chromosomes, Plant; D055864:Droughts; D007178:Inbreeding; D009997:Osmotic Pressure; D010641:Phenotype; D040641:Quantitative Trait Loci; D036226:Seedlings / Q000235:genetics / Q000254:growth & development; D014908:Triticum / Q000235:genetics* / Q000254:growth & development
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