title: Capsaicin modulates proliferation, migration, and activation of hepatic stellate cells.
abstract: Capsaicin, the active component of chili pepper, has been reported to have antiproliferative and anti-inflammatory effects on a variety of cell lines. In the current study, we aimed to investigate the effects of capsaicin during HSC activation and maintenance. Activated and freshly isolated HSCs were treated with capsaicin. Proliferation was measured by incorporation of EdU. Cell cycle arrest and apoptosis were investigated using flow cytometry. The migratory response to chemotactic stimuli was evaluated by a modified Boyden chamber assay. Activation markers and inflammatory cytokines were determined by qPCR, immunocytochemistry, and flow cytometry. Our results show that capsaicin reduces HSC proliferation, migration, and expression of profibrogenic markers of activated and primary mouse HSCs. In conclusion, the present study shows that capsaicin modulates proliferation, migration, and activation of HSC in vitro.
authors: Laboratório de Pesquisa em Biofísica Celular e Inflamação, Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS), Avenida Ipiranga 6681, prédio 12, bloco C, sala 221, Porto Alegre, RS, CEP 90619-900, Brazil.
authors: Shanna
authors: S
authors: Bitencourt
authors: Fernanda
authors: F
authors: Mesquita
authors: Bruno
authors: B
authors: Basso
authors: Júlia
authors: J
authors: Schmid
authors: Gabriela
authors: G
authors: Ferreira
authors: Lucas
authors: L
authors: Rizzo
authors: Moises
authors: M
authors: Bauer
authors: Ramon
authors: R
authors: Bartrons
authors: Francesc
authors: F
authors: Ventura
authors: Jose Luis
authors: JL
authors: Rosa
authors: Inge
authors: I
authors: Mannaerts
authors: Leo Adrianus
authors: LA
authors: van Grunsven
authors: Jarbas
authors: J
authors: Oliveira
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002211:Capsaicin / Q000494:pharmacology*; D059447:Cell Cycle Checkpoints / Q000187:drug effects; D002460:Cell Line; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D016207:Cytokines / Q000378:metabolism; D055166:Hepatic Stellate Cells / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D020782:Matrix Metalloproteinases / Q000378:metabolism; D051379:Mice
chemical_list: D016207:Cytokines; D020782:Matrix Metalloproteinases; D002211:Capsaicin