title: The use of laryngeal mucosa mesenchymal stem cells for the repair the vocal fold injury.
abstract: Stem cell transplantation is a kind of attractive and new approach that complements traditional restorative or surgical techniques for the regeneration of injured or pathologically damaged laryngeal tissues. However, the best cell delivery strategy remains to be identified. The objective of this study was to establish a new strategy to the healing of injured vocal fold, using laryngeal mucosa mesenchymal stem cells differentiating into myofibroblasts or fibroblasts and improving the reconstruction microenvironment in the vocal fold injury as a new alternative as seed cells for laryngeal tissue engineering. After isolation and expansion, cells were identified as adherent mesenchymal cells with substantial proliferation potential in vitro, and were also characterized by flow cytometry. The differentiation potential of mesenchymal cells was maintained during proliferation as confirmed by culturing for adipogenesis, osteogenesis and chondrocyte. When LM-MSC was transplanted into the injured vocal fold, it has the potent differentiated into myofibroblasts and fibroblasts, which could regulate extracellular matrix, block collagen and the fibronectin rapid increased, inhibit the rapidly decrease of elastic fiber and HA, decrease the microenvironment inflammatory reaction, and prevent the formation of vocal fold scar.
authors: Department of Otolaryngology, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi 710032, China; Research and Development Center for Tissue Engineering, Fourth Military Medical University, Xi'an, Shaanxi 710032, China.
authors: Han
authors: H
authors: Peng
authors: Leiguo
authors: L
authors: Ming
authors: Runqin
authors: R
authors: Yang
authors: Yang
authors: Y
authors: Liu
authors: Yuanyuan
authors: Y
authors: Liang
authors: Yu
authors: Y
authors: Zhao
authors: Yan
authors: Y
authors: Jin
authors: Zhihong
authors: Z
authors: Deng
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D048430:Cell Shape; D002470:Cell Survival; D002921:Cicatrix / Q000473:pathology; D004195:Disease Models, Animal; D004285:Dogs; D005109:Extracellular Matrix / Q000378:metabolism; D005260:Female; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D007820:Laryngeal Mucosa / Q000166:cytology*; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D014827:Vocal Cords / Q000293:injuries* / Q000473:pathology*; D014945:Wound Healing
keywords: Epiglottis; Laryngeal mucosa; Mesenchymal stem cells; Scar; Vocal fold