title: HPLC and HPTLC methods by design for quantitative characterization and in vitro anti-oxidant activity of polyherbal formulation containing Rheum emodi.
abstract: Safoof-e-Pathar phori (SPP) is a traditional polyherbal formulation and has been used since long time for its anti-urolithiatic activity. It contains three plant constituents Didymocarpous pedicellata, Dolichous biflorus and Rheum emodi. Emodin and chrysophanic acid were selected as chemical markers for SPP and quantified using simultaneous HPTLC and RP-HPLC methods in R. emodi and in SPP. The simultaneous methods were found linear r(2) = 0.991 in a wide range (10-800 ng spot(-1) with HPTLC, 5-500 µg mL(-1) with HPLC) precise, accurate and robust for both the drugs. Anti-oxidant activity of SPP, R. emodi as well as standard emodin and chrysophanic acid were determined by using DPPH (2,2-diphenyl-1-picryl hydrazyl radical), which showed better activity of R. emodi (IC50 = 12.27) extract when compared with SPP (IC50 = 32.99) and standard drugs (IC50 = 66.81). The robustness of methods were proved by applying the Box-Behnken response surface design software and other validation parameters evaluated were satisfactorily met; hence, the developed method found suitable for application in the quality control of several formulations containing emodin and chrysophanic acid.
authors: Bioactive Natural Product Laboratory, Department of Pharmacognosy and Phytochemistry, Faculty of Pharmacy, Jamia Hamdard (Hamdard University), Jamia Hamdard, New Delhi 110062, India.
authors: Wasim
authors: W
authors: Ahmad
authors: Department of Surgery, Faculty of Medicine, Jamia Hamdard (Hamdard University), New Delhi 110062, India.
authors: Syed Mohammad Arif
authors: SM
authors: Zaidi
authors: Bioactive Natural Product Laboratory, Department of Pharmacognosy and Phytochemistry, Faculty of Pharmacy, Jamia Hamdard (Hamdard University), Jamia Hamdard, New Delhi 110062, India.
authors: Mohd
authors: M
authors: Mujeeb
authors: Bioactive Natural Product Laboratory, Department of Pharmacognosy and Phytochemistry, Faculty of Pharmacy, Jamia Hamdard (Hamdard University), Jamia Hamdard, New Delhi 110062, India.
authors: Shahid Hussain
authors: SH
authors: Ansari
authors: Bioactive Natural Product Laboratory, Department of Pharmacognosy and Phytochemistry, Faculty of Pharmacy, Jamia Hamdard (Hamdard University), Jamia Hamdard, New Delhi 110062, India sahmad_jh@yahoo.co.in.
authors: Sayeed
authors: S
authors: Ahmad
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D010936:Plant Extracts / Q000737:chemistry*; D015203:Reproducibility of Results; D012250:Rheum / Q000737:chemistry*
chemical_list: D010936:Plant Extracts