title: Immunotherapy of CD30-expressing lymphoma using a highly stable ssDNA aptamer.
abstract: CD30 is highly expressed on Hodgkins lymphoma and anaplastic large cell lymphoma, making it an attractive target for therapy. We describe the generation of serum-stabilized ssDNA aptamers that bind CD30 via a hybrid SELEX methodology. The selected aptamer bound CD30 with high affinity and specificity. Further optimization of the aptamer led to a short, truncated variant with a 50-fold higher affinity than its longer counterpart. The multivalent aptamer was able to induce oligomerization of CD30 receptors and, in effect, activate downstream signaling, which led to apoptosis of ALCL cells. Immunotherapy using aptamer-based co-stimulation provides an alternative to antibodies, and has potential to transform cancer treatment.
authors: Department of Pathology and Genomic Medicine, Houston Methodist Hospital, 6565 Fannin St., Houston, TX 77030, USA.
authors: Parag
authors: P
authors: Parekh
authors: Sanchit
authors: S
authors: Kamble
authors: Nianxi
authors: N
authors: Zhao
authors: Zihua
authors: Z
authors: Zeng
authors: Bryce P
authors: BP
authors: Portier
authors: Youli
authors: Y
authors: Zu
mesh_terms: D017209:Apoptosis; D052157:Aptamers, Nucleotide / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D045744:Cell Line, Tumor; D018922:HL-60 Cells; D006689:Hodgkin Disease / Q000235:genetics / Q000473:pathology; D006801:Humans; D007167:Immunotherapy / Q000379:methods*; D020014:K562 Cells; D017730:Ki-1 Antigen / Q000235:genetics / Q000378:metabolism*; D017728:Lymphoma, Large-Cell, Anaplastic / Q000235:genetics / Q000473:pathology*; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D017422:Sequence Analysis, DNA; D015398:Signal Transduction
chemical_list: D052157:Aptamers, Nucleotide; D017730:Ki-1 Antigen
keywords: ALCL; CD30; Cancer immunotherapy; Lymphoma; cHL; ssDNA aptamer
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