title: Characterization of multiple myeloma vesicles by label-free relative quantitation.
abstract: Multiple myeloma (MM) is a hematological malignancy caused by a microenviromentally aided persistence of plasma cells in the bone marrow. The role that extracellular vesicles (EVs), microvesicles and exosomes, released by MM cells have in cell-to-cell communication and signaling in the bone marrow is currently unknown. This paper describes the proteomic content of EVs derived from MM.1S and U266 MM cell lines. First, we compared the protein identifications between the vesicles and cellular lysates of each cell line finding a large overlap in protein identifications. Next, we applied label-free spectral count quantitation to determine proteins with differential abundance between the groups. Finally, we used bioinformatics to categorize proteins with significantly different abundances into functional groups. The results illustrate the first use of label-free spectral counting applied to determine relative protein abundances in EVs.
authors: Department of Molecular Virology, Immunology and Medical Genetics, The Ohio State University, Columbus, OH, USA; Comprehensive Cancer Center, The Ohio State University, Columbus, OH, USA.
authors: Sean W
authors: SW
authors: Harshman
authors: Alessandro
authors: A
authors: Canella
authors: Paul D
authors: PD
authors: Ciarlariello
authors: Alberto
authors: A
authors: Rocci
authors: Kitty
authors: K
authors: Agarwal
authors: Emily M
authors: EM
authors: Smith
authors: Tiffany
authors: T
authors: Talabere
authors: Yvonne A
authors: YA
authors: Efebera
authors: Craig C
authors: CC
authors: Hofmeister
authors: Don M
authors: DM
authors: Benson
authors: Michael E
authors: ME
authors: Paulaitis
authors: Michael A
authors: MA
authors: Freitas
authors: Flavia
authors: F
authors: Pichiorri
mesh_terms: D002457:Cell Extracts; D045744:Cell Line, Tumor; D002853:Chromatography, Liquid; D022162:Cytoplasmic Vesicles / Q000378:metabolism* / Q000648:ultrastructure; D006801:Humans; D013058:Mass Spectrometry; D009101:Multiple Myeloma / Q000378:metabolism* / Q000648:ultrastructure; D009363:Neoplasm Proteins / Q000378:metabolism; D040901:Proteomics; D015203:Reproducibility of Results; D013194:Staining and Labeling / Q000379:methods*
chemical_list: D002457:Cell Extracts; D009363:Neoplasm Proteins
keywords: Cell biology; Exosomes; LC-MS/MS; Label free; Microvesicles
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