title: Surveillance of human influenza A(H3N2) virus from 1999 to 2009 in southern Italy.
abstract: The aim of this study was to evaluate the presence of influenza virus co-infections in humans and changes in the genetic variability of A(H3N2) virus strains in southern Italy from 1999 to 2009. A partial sequence of the haemagglutinin (HA) gene by human influenza H3N2 strains identified in oropharyngeal swabs from patients with influenza-like illness was analysed by DNA sequencing and a phylogenetic analysis was performed. During the seasons 1999-2000, 2002-2003, 2004-2005 and 2008-2009, the influenza viruses circulating belonged to subtype H3N2. However, A(H1N1) subtype virus and B type were respectively prevalent during the 2000-2001, 2006-2007, 2007-2008 and 2001-2002, 2003-2004, 2005-2006 seasons. The HA sequences appeared to be closely related to the sequence of the influenza A vaccine strain. Only the 2002-2003 season was characterized by co-circulation of two viral lineages: A/New York/55/01(H3N2)-like virus of the previous season and A/Fujian/411/02(H3N2)-like virus, a new H3 variant. In this study, over the decade analysed, no significant change was seen in the sequences of the HA gene of H3 viruses isolated.
authors: Laboratory of Hygiene, Department of Biological and Environmental Sciences and Technologies, University of Salento, Lecce, Italy.
authors: A
authors: A
authors: DE Donno
authors: Laboratory of Hygiene, Department of Biological and Environmental Sciences and Technologies, University of Salento, Lecce, Italy.
authors: A
authors: A
authors: Idolo
authors: Laboratory of Hygiene, Department of Biological and Environmental Sciences and Technologies, University of Salento, Lecce, Italy.
authors: M
authors: M
authors: Quattrocchi
authors: Institute of Clinical Physiology, National Council of Research, Lecce, Italy.
authors: A
authors: A
authors: Zizza
authors: Department of Clinical and Experimental Medicine, Section of Hygiene and Occupational Health, University of Ferrara, Italy.
authors: G
authors: G
authors: Gabutti
authors: Laboratory of General Physiology, Department of Biological and Environmental Sciences and Technologies, University of Salento, Lecce, Italy.
authors: A
authors: A
authors: Romano
authors: Division of Infectious Diseases, HIV Center, S. Caterina Novella Hospital, Galatina, Lecce, Italy.
authors: P
authors: P
authors: Grima
authors: Department of Infectious, Parasitic and Immunomediate Diseases, Istituto Superiore di Sanità, Rome, Italy.
authors: I
authors: I
authors: Donatelli
authors: Laboratory of Hygiene, Department of Biological and Environmental Sciences and Technologies, University of Salento, Lecce, Italy.
authors: M
authors: M
authors: Guido
mesh_terms: D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000235:genetics; D006801:Humans; D053122:Influenza A Virus, H3N2 Subtype / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D007251:Influenza, Human / Q000453:epidemiology* / Q000821:virology*; D007558:Italy / Q000453:epidemiology; D010802:Phylogeny; D062486:Public Health Surveillance
chemical_list: D019267:Hemagglutinin Glycoproteins, Influenza Virus; C483468:hemagglutinin, human influenza A virus
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