title: Heat treatment of egg white controls allergic symptoms and induces oral tolerance to ovalbumin in a murine model of food allergy.
abstract: METHODS
  Heated foods often present low allergenicity, and have recently been used in specific oral immunotherapy for food allergies. However, the influence of heating on tolerogenicity of food allergens is not well elucidated. Here, we investigated biochemical, allergenic, and tolerogenic properties of heated egg white (EW) using a murine model of food allergy.
  
  
  RESULTS
  Raw EWs were treated at 80°C for 15 min (80EW, mild heating condition), 100°C for 5 min (100EW, cooking condition), or 121°C for 40 min (121EW, retort pouch condition), and freeze-dried. A transgenic OVA23-3 mice model expressing T-cell receptor specific for ovalbumin (OVA, a major EW allergen) induced Th2 cells and IgE production, and presented intestinal inflammation when fed untreated EW diet. 80EW-fed mice presented only moderate inflammation but high Th2 responses. 100EW-fed mice did not present inflammation but induced tolerance as seen in reduced T-cell responses and IgE levels. 100EW demonstrated higher digestive stability and slower absorption in intestine, compared with untreated EW and 80EW. 121EW was strongly aggregated, was not absorbed well, and developed Th1 responses without tolerance induction.
  
  
  CONCLUSIONS
  OVA in EW treated only under a particular heat condition (e.g. 100°C for 5 min) lost allergenicity, but possessed tolerogenicity.
authors: Chemistry Division, Kanagawa Prefectural Institute of Public Health, Chigasaki, Japan.
authors: Hiroko
authors: H
authors: Watanabe
authors: Masako
authors: M
authors: Toda
authors: Haruko
authors: H
authors: Sekido
authors: Anne
authors: A
authors: Wellner
authors: Tomoyuki
authors: T
authors: Fujii
authors: Thomas
authors: T
authors: Henle
authors: Satoshi
authors: S
authors: Hachimura
authors: Haruyo
authors: H
authors: Nakajima-Adachi
mesh_terms: D000818:Animals; D049109:Cell Proliferation; D004195:Disease Models, Animal; D004529:Egg White / Q000032:analysis*; D005511:Food Handling / Q000379:methods*; D005512:Food Hypersensitivity / Q000276:immunology* / Q000517:prevention & control; D006358:Hot Temperature; D007108:Immune Tolerance / Q000276:immunology*; D007073:Immunoglobulin E / Q000097:blood; D007249:Inflammation / Q000276:immunology / Q000473:pathology; D007422:Intestines / Q000276:immunology / Q000473:pathology; D051379:Mice; D008822:Mice, Transgenic; D010047:Ovalbumin / Q000009:adverse effects* / Q000097:blood
chemical_list: D007073:Immunoglobulin E; D010047:Ovalbumin
keywords: Egg white; Food allergy; Heat treatment; Mouse model; Oral tolerance