title: An androgen receptor-microrna-29a regulatory circuitry in mouse epididymis.
abstract: MicroRNAs are involved in a number of cellular processes; thus, their deregulation is usually apt to the occurrence of diverse diseases. Previous studies indicate that abnormally up-regulated miR-29a is associated with several diseases, such as human acute myeloid leukemia and diabetes; therefore, the proper level of miR-29a is critical for homeostasis. Herein, we observed that miR-29a was repressed by androgen/androgen receptor signaling in mouse epididymis by targeting a conserved androgen response element located 8 kb upstream of miR-29b1a loci. It is well known that multiple regulatory programs often form a complicated network. Here, we found that miR-29a reversibly suppressed androgen receptor and its target genes by targeting IGF1 and p53 pathways. miR-29b1a-overexpressing transgenic mice displayed epididymis hypoplasia partially similar to the phenotype of those mice with an impaired androgen-androgen receptor signal system. Taken together, the results demonstrated that there is a regulatory circuitry between the androgen signaling pathway and miR-29a in mouse epididymis that may be vital for epididymal development and functions.
authors: From the Shanghai Key Laboratory for Molecular Andrology, State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Shanghai 200031, China.
authors: Wubin
authors: W
authors: Ma
authors: Shuanggang
authors: S
authors: Hu
authors: Guangxin
authors: G
authors: Yao
authors: Shengsong
authors: S
authors: Xie
authors: Minjie
authors: M
authors: Ni
authors: Qiang
authors: Q
authors: Liu
authors: Xinxing
authors: X
authors: Gao
authors: Jun
authors: J
authors: Zhang
authors: Xingxu
authors: X
authors: Huang
authors: Yonglian
authors: Y
authors: Zhang
mesh_terms: D000728:Androgens / Q000494:pharmacology; D000818:Animals; D015153:Blotting, Western; D002460:Cell Line; D045744:Cell Line, Tumor; D004822:Epididymis / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000187:drug effects; D057809:HEK293 Cells; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000235:genetics / Q000378:metabolism; D061986:MCF-7 Cells; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D035683:MicroRNAs / Q000235:genetics*; D008957:Models, Genetic; D009919:Orchiectomy; D034622:RNA Interference; D051381:Rats; D017207:Rats, Sprague-Dawley; D011944:Receptors, Androgen / Q000235:genetics* / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction / Q000235:genetics*; D016159:Tumor Suppressor Protein p53 / Q000235:genetics / Q000378:metabolism
chemical_list: D000728:Androgens; C547014:MIRN29 microRNA, mouse; D035683:MicroRNAs; D011944:Receptors, Androgen; D016159:Tumor Suppressor Protein p53; C519530:insulin-like growth factor-1, mouse; D007334:Insulin-Like Growth Factor I
keywords: Androgen; Androgen Receptor; Epididymis; Gene Regulation; MicroRNA
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