title: Exploration and comparison of inborn capacity of aerobic and anaerobic metabolisms of Saccharomyces cerevisiae for microbial electrical current production.
abstract: Saccharomyces cerevisiae possesses numerous advantageous biological features, such as being robust, easily handled, mostly non-pathogenic and having high catabolic rates, etc., which can be considered as merits for being used as a promising biocatalyst in microbial fuel cells (MFCs) for electricity generation. Previous studies have developed efficient MFC configurations to convert metabolic electron shuttles, such as cytoplasmic NADH, into usable electric current. However, no studies have elucidated the maximum potential of S. cerevisiae for current output and the underlying metabolic pathways, resulting from the interaction of thousands of reactions inside the cell during MFC operation. To address these two key issues, this study used in silico metabolic engineering techniques, flux balance analysis (FBA), and flux variability analysis with target flux minimization (FATMIN), to model the metabolic perturbation of S. cerevisiae under the MFC-energy extraction. The FBA results showed that, in the cytoplasmic NADH-dependent mediated electron transfer (MET) mode, S. cerevisiae had a potential to produce currents at up to 5.781 A/gDW for the anaerobic and 6.193 A/gDW for the aerobic environments. The FATMIN results showed that the aerobic and anaerobic metabolisms are resilient, relying on six and five contributing reactions respectively for high current production. Two reactions, catalyzed by glutamate dehydrogenase (NAD) (EC 1.4.1.3) and methylene tetrahydrofolate dehydrogenase (NAD) (EC 1.5.1.5), were shared in both current-production modes and contributed to over 80% of the identified maximum current outputs. It is also shown that the NADH regeneration was much less energy costly than biomass production rate. Taken together, our finding suggests that S. cerevisiae should receive more research effort for MFC electricity production.
authors: Centre for Advanced Computational Solutions; Department of Molecular Biosciences; Lincoln University; Lincoln, New Zealand.
authors: Longfei
authors: L
authors: Mao
authors: Centre for Advanced Computational Solutions; Department of Molecular Biosciences; Lincoln University; Lincoln, New Zealand.
authors: Wynand S
authors: WS
authors: Verwoerd
mesh_terms: D000332:Aerobiosis; D000693:Anaerobiosis; D001674:Bioelectric Energy Sources; D018533:Biomass; D004560:Electricity; D004579:Electron Transport; D005969:Glutamate Dehydrogenase / Q000378:metabolism; D060847:Metabolic Engineering; D053858:Metabolic Networks and Pathways; D045102:Methylenetetrahydrofolate Dehydrogenase (NAD+) / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000378:metabolism*
chemical_list: D005969:Glutamate Dehydrogenase; D045102:Methylenetetrahydrofolate Dehydrogenase (NAD+)
keywords: FATMIN; MFC; Saccharomyces cerevisiae; bioelectricity; flux balance analysis; flux minimization; flux variability analysis; microbial fuel cell
references: Microbiology (Reading). 2003 Aug;149(Pt 8):2137-2145
references: 12904553
references: Genes Dev. 1998 Aug 15;12(16):2560-73
references: 9716408
references: Biosens Bioelectron. 2011 May 15;26(9):3742-7
references: 21493057
references: J Ind Microbiol Biotechnol. 2013 Oct;40(10):1161-80
references: 23851491
references: Anal Biochem. 2009 Jan 1;384(1):11-9
references: 18812160
references: Bioelectrochemistry. 2012 Aug;86:97-101
references: 22357359
references: Environ Sci Technol. 2006 Sep 1;40(17):5181-92
references: 16999087
references: Drug Discov Today. 2010 Jun;15(11-12):451-60
references: 20438859
references: Nat Rev Microbiol. 2012 Feb 27;10(4):291-305
references: 22367118
references: Appl Environ Microbiol. 1994 Oct;60(10):3724-31
references: 7986045
references: Bioresour Technol. 2011 Mar;102(5):4144-9
references: 21216140
references: Genetics. 1992 Dec;132(4):963-73
references: 1459447
references: C R Biol. 2011 Aug-Sep;334(8-9):687-93
references: 21819951
references: Environ Sci Technol. 2006 Sep 1;40(17):5193-9
references: 16999088
references: J Biol Eng. 2013 May 29;7(1):14
references: 23718629
references: Trends Biotechnol. 2005 Jun;23(6):291-8
references: 15922081
references: Phys Chem Chem Phys. 2007 Jun 7;9(21):2619-29
references: 17627307
references: Nat Protoc. 2011 Aug 04;6(9):1290-307
references: 21886097
references: Environ Sci Technol. 2006 Apr 1;40(7):2426-32
references: 16646485
references: BMC Syst Biol. 2009 Mar 25;3:37
references: 19321003
references: Nat Genet. 2004 Oct;36(10):1056-8
references: 15448692
references: BMC Syst Biol. 2012 Jun 04;6:55
references: 22663945
references: Int J Mol Sci. 2008 Oct;9(10):1893-907
references: 19325724
references: J Biosci Bioeng. 2014 Nov;118(5):565-74
references: 24875305
references: Nature. 2002 Nov 14;420(6912):186-9
references: 12432395
references: Mol Syst Biol. 2009;5:301
references: 19690570
references: PLoS One. 2011 Feb 02;6(2):e16015
references: 21311596
references: Proc Natl Acad Sci U S A. 2003 Nov 11;100(23):13134-9
references: 14578455
references: J Biosci Bioeng. 2011 Oct;112(4):379-87
references: 21782506
references: Nat Biotechnol. 2001 Feb;19(2):125-30
references: 11175725
references: Biotechnol Bioeng. 2003 Jun 20;82(6):670-7
references: 12673766
references: Biotechnol J. 2010 Feb;5(2):147-62
references: 20084640
references: Biotechnol Bioeng. 1975 Oct;17(10):1529-43
references: 241440
references: Nature. 2000 Oct 5;407(6804):651-4
references: 11034217
references: BMC Syst Biol. 2010 Apr 19;4:45
references: 20403172
references: Biosens Bioelectron. 2006 May 15;21(11):2015-45
references: 16569499
references: Bioresour Technol. 2011 Feb;102(3):2751-7
references: 21146401
references: Proc Natl Acad Sci U S A. 2000 Apr 11;97(8):4186-91
references: 10760286
references: Nat Biotechnol. 2010 Mar;28(3):245-8
references: 20212490
references: Mol Syst Biol. 2009;5:320
references: 19888215
references: BMC Syst Biol. 2010 Dec 29;4:178
references: 21190580
references: Appl Microbiol Biotechnol. 2004 Feb;63(6):672-81
references: 12908088
references: Appl Environ Microbiol. 2000 Apr;66(4):1292-7
references: 10742202