title: Morin inhibits sortase A and subsequent biofilm formation in Streptococcus mutans.
abstract: Streptococcus mutans (S. mutans) is the main etiological agent of dental caries, and adheres to the tooth surface through the sortase A (SrtA)-mediated cell wall-anchored protein Pac. Inhibition of SrtA activity results in a marked reduction in the adhesion potential of S. mutans, and the frequency of dental caries. Morin is a natural plant extract that was previously reported to inhibit Staphylococcus aureus SrtA activity. Here, we demonstrate that morin has an inhibitory effect against S. mutans UA159 SrtA, with an IC50 of 27.2 ± 2.6 μM. Western blotting demonstrated that 30 μM morin induced the partial release of the Pac protein into the supernatant. The biofilm mass of S. mutans was reduced in the presence of 30 μM morin, which was not caused by a decrease in S. mutans viability. These results indicate that morin might be important as a new agent to prevent caries.
authors: Center of Stomatology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, No. 1095, Jiefang Avenue, Wuhan, 430030, People's Republic of China.
authors: Ping
authors: P
authors: Huang
authors: Ping
authors: P
authors: Hu
authors: Su Yun
authors: SY
authors: Zhou
authors: Qian
authors: Q
authors: Li
authors: Wei Min
authors: WM
authors: Chen
mesh_terms: D000595:Amino Acid Sequence; D019881:Aminoacyltransferases / Q000187:drug effects* / Q000378:metabolism; D000704:Analysis of Variance; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000187:drug effects* / Q000378:metabolism; D018441:Biofilms / Q000187:drug effects*; D002473:Cell Wall; D003546:Cysteine Endopeptidases / Q000187:drug effects* / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000494:pharmacology*; D005419:Flavonoids / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D050296:Microbial Viability / Q000187:drug effects; D008969:Molecular Sequence Data; D012463:Saliva / Q000382:microbiology; D013295:Streptococcus mutans / Q000187:drug effects* / Q000201:enzymology / Q000302:isolation & purification; D047309:Flavones
chemical_list: D019881:Aminoacyltransferases; D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D003546:Cysteine Endopeptidases; D004791:Enzyme Inhibitors; D005419:Flavonoids; C008548:morin; D047309:Flavones; C120542:sortase A
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