title: New insights into cell cycle and DNA damage response machineries through high-resolution AMICO quantitative imaging cytometry.
abstract: OBJECTIVE
  Progress in biology and medicine research is being driven by development of new instrumentation and associated methodologies which open analytical capabilities that expand understanding of complexity of biological systems. Application of cytometry, which is now widely used in so many disciplines of biology, is the best example of such a progress.
  
  
  METHODS
  Recent publications push the envelope in expanding capabilities of cytometry by introducing a high resolution imaging cytometry defined as Automated Microscopy for Image CytOmetry (AMICO). This instrumentation is utilized to further elucidate mechanisms of the cell cycle progression and also the DNA damage response. This approach is going beyond the presently possible analytical technologies regarding throughput and depth of information.
  
  
  CONCLUSIONS
  The possibility of multiparametric analysis combined with the high resolution mapping of individual constituents of cell cycle and DNA damage response machineries provides new tools to probe molecular mechanism of these processes. The capability of analysis of proximity of these constituents to each other offered by AMICO is a novel and potentially important approach that can be used to elucidate mechanisms of other biological processes.
authors: Department of Paediatric Cardiology, Cardiac Centre, and Translational Centre for Regenerative Medicine (TRM), Universität Leipzig, 04289, Leipzig, Germany.
authors: A
authors: A
authors: Tarnok
authors: Z
authors: Z
authors: Darzynkiewicz
mesh_terms: D001331:Automation; D002460:Cell Line; D057930:Click Chemistry; D004249:DNA Damage; D004260:DNA Repair; D004261:DNA Replication; D005434:Flow Cytometry; D006657:Histones / Q000378:metabolism; D006801:Humans; D007399:Interphase; D015335:Molecular Probes / Q000737:chemistry; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism
chemical_list: C468783:H2AX protein, human; D006657:Histones; D015335:Molecular Probes; D016159:Tumor Suppressor Protein p53
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