title: Expanding the scope of site-specific recombinases for genetic and metabolic engineering.
abstract: Site-specific recombinases are tremendously valuable tools for basic research and genetic engineering. By promoting high-fidelity DNA modifications, site-specific recombination systems have empowered researchers with unprecedented control over diverse biological functions, enabling countless insights into cellular structure and function. The rigid target specificities of many sites-specific recombinases, however, have limited their adoption in fields that require highly flexible recognition abilities. As a result, intense effort has been directed toward altering the properties of site-specific recombination systems by protein engineering. Here, we review key developments in the rational design and directed molecular evolution of site-specific recombinases, highlighting the numerous applications of these enzymes across diverse fields of study.
authors: The Skaggs Institute for Chemical Biology and the Departments of Chemistry and Cell and Molecular Biology, The Scripps Research Institute, La Jolla, California, 92037.
authors: Thomas
authors: T
authors: Gaj
authors: Shannon J
authors: SJ
authors: Sirk
authors: Carlos F
authors: CF
authors: Barbas
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D005818:Genetic Engineering; D006801:Humans; D060847:Metabolic Engineering; D051379:Mice; D008958:Models, Molecular; D008969:Molecular Sequence Data; D045522:Recombinases
chemical_list: D045522:Recombinases
keywords: genome engineering; protein engineering; recombinase
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