title: Impairment by sperm agglutinating factor isolated from Escherichia coli: receptor specific interactions.
abstract: In an earlier work done in our laboratory, we have been able to isolate a sperm agglutinating strain of Escherichia coli from the semen sample of a male attending infertility clinic. Further, factor responsible for sperm agglutination (SAF) was isolated and purified, and, using SAF as a tool, corresponding SAF binding receptor from human spermatozoa has been purified. Characterization of SAF and SAF binding receptor using MALDI-TOF showed homology to glutamate decarboxylase and MHC class I molecule, respectively. Coincubation of SAF with spermatozoa not only resulted in spermagglutination but could also compromise other sperm parameters, namely, Mg(2+) dependent ATPase activity and apoptosis. Intravaginal administration of SAF could lead to infertility in Balb/c mice. SAF induced impairment of sperm parameters, and infertility was observed to be due to interaction of SAF with sperm surface receptor component as, when purified receptor was introduced, receptor completely inhibited all the detrimental effects induced by SAF. From these results, it could be concluded that interaction of SAF with spermatozoa is receptor mediated.
authors: Department of Microbiology, Panjab University, Chandigarh 160014, India.
authors: Kiranjeet
authors: K
authors: Kaur
authors: Vijay
authors: V
authors: Prabha
mesh_terms: D000251:Adenosine Triphosphatases / Q000378:metabolism; D000818:Animals; D017209:Apoptosis / Q000235:genetics; D004926:Escherichia coli / Q000737:chemistry / Q000378:metabolism; D005968:Glutamate Decarboxylase / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D007248:Infertility, Male / Q000235:genetics* / Q000378:metabolism; D008285:Major Histocompatibility Complex / Q000235:genetics*; D008297:Male; D051379:Mice; D011485:Protein Binding; D012661:Semen / Q000378:metabolism; D013073:Sperm Agglutination / Q000235:genetics*; D013094:Spermatozoa / Q000378:metabolism / Q000473:pathology; D013211:Staphylococcus aureus
chemical_list: D000251:Adenosine Triphosphatases; C035123:magnesium sodium ATPase; D005968:Glutamate Decarboxylase
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