title: Identification and analysis of the constituents in an aqueous extract of Tricholoma matsutake by HPLC coupled with diode array detection/electrospray ionization mass spectrometry.
abstract: The main constituents in an aqueous extract of Tricholoma matsutake (Tm) were identified by high-performance liquid chromatography coupled with diode array detection and electrospray ionization time-of-flight mass spectrometry (HPLC-DAD/TOF-MS) and ion trap mass spectrometry (HPLC-DAD/Trap-MSn). The main factors in the extraction process which affect the yields of nutrients were optimized by single-factor experiments and orthogonal experiment design. In total, 12 constituents were identified from the aqueous extract of Tm, including tyrosine, cytidine, uridine, eritadenine, phenylalanine, nicotinamide, inosine, guanosine, tryptophan, adenosine, 5'-deoxy-5'-methylthioadenosine and riboflavin. The optimized extraction conditions were: the ratio of water to sample was 10 : 1 (v/w), Tm was extracted by ultrasonic-assisted extraction for 10 min, followed by water bath heating at 60 °C for 1 h. Among these extraction factors, the heating temperature is significant based on analysis of variance (ANOVA). The yields of nutrients were affected dramatically at high temperature leading to the loss of nutrients, especially for nucleosides and some amino acids.
authors: College of Pharmacy of Nankai Univ, Tianjin 300071, China; Analysis Centre of Tsinghua Univ, Beijing 100084, China.
authors: Xuhui
authors: X
authors: Ying
authors: Jinfang
authors: J
authors: Ma
authors: Qionglin
authors: Q
authors: Liang
authors: Yiming
authors: Y
authors: Wang
authors: Gang
authors: G
authors: Bai
authors: Guoan
authors: G
authors: Luo
mesh_terms: D000225:Adenine / Q000031:analogs & derivatives / Q000302:isolation & purification; D000241:Adenosine / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003562:Cytidine / Q000302:isolation & purification; D003839:Deoxyadenosines / Q000302:isolation & purification; D006151:Guanosine / Q000302:isolation & purification; D007288:Inosine / Q000302:isolation & purification; D010649:Phenylalanine / Q000302:isolation & purification; D012256:Riboflavin / Q000302:isolation & purification; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D013872:Thionucleosides / Q000302:isolation & purification; D055439:Tricholoma / Q000737:chemistry*; D014364:Tryptophan / Q000302:isolation & purification; D014443:Tyrosine / Q000302:isolation & purification; D014529:Uridine / Q000302:isolation & purification; D014867:Water / Q000737:chemistry
chemical_list: D003839:Deoxyadenosines; D013872:Thionucleosides; D014867:Water; D006151:Guanosine; C100029:eritadenine; D014443:Tyrosine; D010649:Phenylalanine; D007288:Inosine; D003562:Cytidine; C008500:5'-methylthioadenosine; D014364:Tryptophan; D000225:Adenine; D000241:Adenosine; D012256:Riboflavin; D014529:Uridine
keywords: LC-MS; Tricholoma matsutake; nucleosides; nutrients; orthogonal design