title: Determination of avilamycin as dichloroisoeverninic acid in poultry and porcine muscles by isotope dilution liquid chromatography-tandem mass spectrometry.
abstract: Avilamycin residue in food is regulated as its marker residue dichloroisoeverninic acid (DIA). An isotope dilution liquid chromatography-tandem mass spectrometry method is established for the accurate determination of DIA in animal muscles without any pre-extraction and preconcentration prior to alkaline hydrolysis. Optimization of the sample cleanup procedures such as liquid-liquid extraction and solid phase extraction was performed by fine-tuning several critical parameters to reduce the matrix effects. Quantification of DIA in edible muscle was accomplished by using matrix-matched calibration with dichloroisoeverninic acid-d6 as internal standard. The method was validated with DIA and avilamycin-fortified poultry and porcine muscles at three different levels (25, 50, and 100 μg/kg). Conversion of avilamycin to DIA by alkaline hydrolysis was ≥92%. The recoveries of DIA in both muscles at three fortification levels ranged from 94 to 106% and RSDs were ≤11% in all cases. The estimated limit of detection values in poultry and porcine muscles were 2.7 and 0.7 μg/kg, respectively. The estimated limit of quantitation values in poultry and porcine muscles were 8.3 and 2.4 μg/kg, respectively. This method is suitable for routine monitoring of avilamycin residue in food safety surveillance programs.
authors: Analytical and Advisory Services Division, Government Laboratory, 7/F, Ho Man Tin Government Offices, 88, Chung Hau Street, Ho Man Tin, Kowloon, Hong Kong, China.
authors: Clare
authors: C
authors: Ho
authors: Yiu-Tung
authors: YT
authors: Wong
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis*; D002713:Chlorine / Q000032:analysis; D002853:Chromatography, Liquid / Q000379:methods*; D007554:Isotopes / Q000032:analysis; D057230:Limit of Detection; D059625:Liquid-Liquid Extraction; D008460:Meat / Q000032:analysis*; D009132:Muscles / Q000737:chemistry; D009844:Oligosaccharides / Q000032:analysis*; D010226:Parabens / Q000032:analysis*; D011200:Poultry; D052616:Solid Phase Extraction; D013552:Swine; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D000900:Anti-Bacterial Agents; D007554:Isotopes; D009844:Oligosaccharides; D010226:Parabens; C436979:dichloroisoeverninic acid; D002713:Chlorine; C006653:avilamycin