title: Inhibitory effects of cultured Dendrobium tosaense on atopic dermatitis murine model.
abstract: Dendrobium tosaense is one of the most valuable Chinese medicines and well developed health food. Atopic dermatitis (AD) is a chronic skin disease that occurs mainly in childhood. The pathogenesis of atopic dermatitis had been studied in BALB/c mice modeling by skin-inoculated ovalbumin (OVA) with 2,4,6-trinitro-1-chrolobenzene (TNCB). These mice exhibit features of chronic dermatitis, including skin rash, mast cells infiltration, and elevated serum anti-OVA specific IgE and cytokines modulation. In this study, a standardized ethyl acetate extract of D. tosaense (DtE) was used to protect these mice from the OVA/TNCB-induced skin lesions of atopic dermatitis. The results indicated an increased population of natural T regulatory cell was accompanied by immunosuppression in cytokine profiles and anti-OVA IgE level to significantly reduce Th2 polarization. Finally, toluidine blue staining indicated mast cell infiltration and degranulation was reduced in skin lesion. Our results were shed light on the usage of D. tosaense in AD.
authors: Department of Agronomy, National Chung-Hsing University, Taichung 40227, Taiwan.
authors: Chin-Tung
authors: CT
authors: Wu
authors: The School of Chinese Medicine for Post-Baccalaureate, I-Shou University, Kaohsiung 84001, Taiwan.
authors: Keng-Shiang
authors: KS
authors: Huang
authors: Department of Biological Science and Technology, I-Shou University, Kaohsiung 84001, Taiwan.
authors: Chih-Hui
authors: CH
authors: Yang
authors: Department of Chinese Pharmaceutical Sciences and Chinese Medicine Resources, China Medical University, Taichung 40402, Taiwan.
authors: Yu-Chang
authors: YC
authors: Chen
authors: Graduate Institute of Veterinary Pathobiology, National Chung-Hsing University, Taichung 40227, Taiwan.
authors: Jiunn-Wang
authors: JW
authors: Liao
authors: Department of Chinese Pharmaceutical Sciences and Chinese Medicine Resources, China Medical University, Taichung 40402, Taiwan.
authors: Chao-Lin
authors: CL
authors: Kuo
authors: Department of Agronomy, National Chung-Hsing University, Taichung 40227, Taiwan.
authors: Chung-Li
authors: CL
authors: Chen
authors: Department of Agronomy, Chiayi Agricultural Experiment Station, TARI, Chiayi 60044, Taiwan.
authors: Shu-Fang
authors: SF
authors: Lo
authors: Department of Chinese Pharmaceutical Sciences and Chinese Medicine Resources, China Medical University, Taichung 40402, Taiwan; Department of Animal Science and Biotechnology, Tunghai University, Taichung 40704, Taiwan. Electronic address: cchsieh@thu.edu.tw.
authors: Chang-Chi
authors: CC
authors: Hsieh
authors: Department of Agronomy, National Chung-Hsing University, Taichung 40227, Taiwan; Department and Graduate Institute of Applied Chemistry, Chaoyang University of Technology, Wufeng 41349, Taiwan. Electronic address: hstsay@cyut.edu.tw.
authors: Hsin-Sheng
authors: HS
authors: Tsay
mesh_terms: D000485:Allergens; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology / Q000627:therapeutic use*; D015550:Cell Degranulation / Q000187:drug effects; D016207:Cytokines / Q000276:immunology; D031667:Dendrobium; D003876:Dermatitis, Atopic / Q000097:blood / Q000139:chemically induced / Q000188:drug therapy* / Q000276:immunology; D004195:Disease Models, Animal; D005260:Female; D007073:Immunoglobulin E / Q000097:blood; D008198:Lymph Nodes / Q000166:cytology; D008407:Mast Cells / Q000187:drug effects / Q000502:physiology; D051379:Mice; D008807:Mice, Inbred BALB C; D010047:Ovalbumin; D008517:Phytotherapy; D010853:Picryl Chloride; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use*; D018547:Plant Stems; D012867:Skin / Q000187:drug effects / Q000276:immunology / Q000473:pathology; D013154:Spleen / Q000166:cytology; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology
chemical_list: D000485:Allergens; D000893:Anti-Inflammatory Agents; D016207:Cytokines; D010936:Plant Extracts; D007073:Immunoglobulin E; D010047:Ovalbumin; D010853:Picryl Chloride
keywords: Atopic dermatitis; Dendrobium tosaense; IgE; Mast cell; T regulatory cell