title: Positive association between urinary levels of 8-hydroxydeoxyguanosine and the acrylamide metabolite N-acetyl-S-(propionamide)-cysteine in adolescents and young adults.
abstract: Acrylamide is present in mainstream cigarette smoke and in some foods prepared at high temperatures. Animal studies have shown that acrylamide exposure increases oxidative stress; however, it is not known if this also occurs in humans. We recruited 800 subjects (mean age, 21.3 years, range, 12-30 years) from a population-based sample of Taiwanese adolescents and young adults to determine if urinary levels of the acrylamide metabolite N-acetyl-S-(propionamide)-cysteine (AAMA) and the oxidative stress product 8-hydroxydeoxyguanosine (8-OHdG) are associated. The mean (SD) AAMA and 8-OHdG were 76.54 (76.42)μg/L and 3.48 (2.37)μg/L, respectively. In linear regression analyses, a 1-unit increase in natural log AAMA was significantly associated with an increase in natural log 8-OHdG (μg/g creatinine) (β=0.044, SE=0.019, P=0.020) after controlling for covariates. Subpopulation analyses showed AAMA and 8-OHdG were significantly associated with males, adolescents, non-current smokers, without alcohol consumption, subjects, body mass index ≥ 24, and homeostasis model assessment of insulin resistance score ≥ 0.9. In conclusion, higher urinary AAMA concentrations were associated with increased levels of urinary 8-OHdG in this cohort. Further studies are warranted to determine if there is a causal relationship between acrylamide exposure and oxidative stress.
authors: Department of Internal Medicine, En Chu Kong Hospital, New Taipei City 237, Taiwan; School of Medicine, Fu Jen Catholic University, New Taipei City 242, Taiwan.
authors: Chien-Yu
authors: CY
authors: Lin
authors: Hui-Ling
authors: HL
authors: Lee
authors: Yu-Chuan
authors: YC
authors: Chen
authors: Guang-Wen
authors: GW
authors: Lien
authors: Lain-Yu
authors: LY
authors: Lin
authors: Li-Li
authors: LL
authors: Wen
authors: Chien-Chang
authors: CC
authors: Liao
authors: Kuo-Liong
authors: KL
authors: Chien
authors: Fung-Chang
authors: FC
authors: Sung
authors: Pau-Chung
authors: PC
authors: Chen
authors: Ta-Chen
authors: TC
authors: Su
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000111:Acetylcysteine / Q000031:analogs & derivatives* / Q000652:urine; D020106:Acrylamide / Q000378:metabolism; D000293:Adolescent; D000328:Adult; D002648:Child; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000652:urine; D004784:Environmental Monitoring; D005260:Female; D006801:Humans; D008297:Male; D013624:Taiwan; D055815:Young Adult
chemical_list: C569465:N-acetyl-S-(2-carbamoylethyl)cysteine; D020106:Acrylamide; C085597:N-acetyl-S-(propionamide)cysteine; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine; D000111:Acetylcysteine
keywords: 8-Hydroxydeoxyguanosine (8-OHdG); 8-OHdG; 8-hydroxydeoxyguanosine; AAMA; Acrylamide; BMI; HDL-C; HOMA-IR; LDL-C; LOD; N-Acetyl-S-(propionamide)-cysteine (AAMA); N-acetyl-S-(2-carbamoylethyl)-cysteine; NTD; NTUH; National Taiwan University Hospital; New Taiwan Dollars; Oxidative stress; ROS; The homeostasis model assessment of insulin resistance; body mass index; high-density lipoprotein cholesterol; limit of detection; low lipoprotein cholesterol; reactive oxygen species