title: Separation of biological proteins by liquid chromatography.
abstract: After the success of human genome project, proteome is a new emerging field of biochemistry as it provides the knowledge of enzymes (proteins) interactions with different body organs and medicines administrated into human body. Therefore, the study of proteomics is very important for the development of new and effective drugs to control many lethal diseases. In proteomics study, analyses of proteome is essential and significant from the pathological point of views, i.e., in several serious diseases such as cancer, Alzheimer's disease and aging, heart diseases and also for plant biology. The separation and identification of proteomics is a challenging job due to their complex structures and closely related physico-chemical behaviors. However, the recent advances in liquid chromatography make this job easy. Various kinds of liquid chromatography, along with different detectors and optimization strategies, have been discussed in this article. Besides, attempts have been made to include chirality concept in proteomics for understanding mechanism and medication of various disease controlled by different body proteins.
authors: Department of Chemistry, Jamia Millia Islamia (Central University), New Delhi 110 025, India.
authors: Imran
authors: I
authors: Ali
authors: Hassan Y
authors: HY
authors: Aboul-Enein
authors: Prashant
authors: P
authors: Singh
authors: Rakesh
authors: R
authors: Singh
authors: Bhavtosh
authors: B
authors: Sharma
keywords: 2D-nano LC, two-dimensional nano liquid chromatography quadrupole; ACN, acetonitrile; AIEC, anion exchange chromatography; CEC, capillary electro-chromatography; CIEF, capillary isoelectric focusing; CSF, cerebrospinal fluid; Chirality; EC, electro-chromatography; ESI-LC–MS, electrospray ionization liquid chromatography–mass spectrometry; FA, formic acid; FLP, FMRF amide-like peptide; FT-ICR-MS, ion cyclotron resonance-mass spectrometry; GPI-APs, glycosylphosphadylinositol anchored proteins; GSH, glutathione stimulating hormone; GSTs, glutathione-S-transferase isoenzyme; Gene; HFBA, heptafluorobutyric acid; HPLC, high performance liquid chromatography; ICAT, isotope coded affinity tag; IEF-SEC, isoelectrofocussing size-exclusion chromatography; IMCD, inner medullary collecting duct; LC-Q-TOF, liquid chromatography-quadrupole time-of-flight tandem mass; LC-dual ESI, liquid chromatography dual electrospray ionization-Fourier transform; LC–MS, liquid chromatography–mass spectrometry; Liquid chromatography; MALDI-TOF, matrix-assisted laser desorption/ionization-time-of flight; MFGM, milk fat globule membranes; MMA, mass measurement accuracy; MPC, mesenchymal progenitor cell; MS/MS, spectrometry; NLFs, Nasal lavage fluids; NLP, neuropeptide like protein; Nano detection; PC2, prohormone convertase-2; PS II, photosystem II; Preparation; Proteomics; Q-TOFMS/MS, time-of-flight tandem-mass spectrometry; RPLC, reversed phase liquid chromatography; SCX, strong cation exchange; SEC, size-exclusion chromatography; TFA, trifluoroacetic acid; TIC, total ion current; TRAF, tumor necrosis factor receptor
references: Semin Thromb Hemost. 2004 Aug;30(4):485-9
references: 15354269
references: J Biochem Biophys Methods. 2003 Jun 30;56(1-3):141-52
references: 12834973
references: Proteomics. 2006 Oct;6(19):5169-82
references: 16947122
references: Invest Ophthalmol Vis Sci. 2006 Apr;47(4):1562-70
references: 16565393
references: J Biol Chem. 2004 Jul 30;279(31):32046-54
references: 15161933
references: Trends Biotechnol. 2002 Dec;20(12 Suppl):S8-13
references: 12570153
references: Proteomics. 2006 Mar;6(5):1437-49
references: 16404722
references: Mol Cell Proteomics. 2005 Jan;4(1):56-72
references: 15576338
references: J Chromatogr A. 2005 May 6;1073(1-2):35-41
references: 15909503
references: Proteomics. 2005 Nov;5(16):4152-60
references: 16196101
references: Expert Rev Proteomics. 2005 Apr;2(2):215-28
references: 15892566
references: J Proteome Res. 2005 Nov-Dec;4(6):2192-200
references: 16335966
references: Mol Cell Proteomics. 2003 Dec;2(12):1319-30
references: 14534352
references: Nat Biotechnol. 2003 Jun;21(6):660-6
references: 12754519
references: Anal Chem. 1997 Apr 15;69(8):1518-24
references: 9109352
references: Mol Cell Proteomics. 2006 May;5(5):845-57
references: 16467180
references: Proteomics. 2004 Nov;4(11):3359-68
references: 15378736
references: Mol Cell Proteomics. 2007 Mar;6(3):548-64
references: 17186946
references: Microb Pathog. 2006 Oct-Nov;41(4-5):157-67
references: 16950595
references: Rapid Commun Mass Spectrom. 2000;14(17):1600-6
references: 10960913
references: J Biol Chem. 2004 Jan 2;279(1):421-8
references: 14561759
references: Mass Spectrom Rev. 2005 Nov-Dec;24(6):918-30
references: 15599945
references: J Proteome Res. 2005 Sep-Oct;4(5):1516-21
references: 16212402
references: Proteomics. 2004 Dec;4(12):3953-9
references: 15526346
references: Proteomics. 2001 Feb;1(2):207-22
references: 11680868
references: Proteomics. 2004 May;4(5):1433-8
references: 15188412
references: Anal Chem. 2002 Feb 15;74(4):809-20
references: 11866061
references: Biochem Biophys Res Commun. 2005 Apr 29;330(1):81-7
references: 15781235
references: J Proteome Res. 2007 Sep;6(9):3501-8
references: 17683130
references: J Proteome Res. 2007 Jul;6(7):2732-45
references: 17559253
references: J Mass Spectrom. 2005 Feb;40(2):176-81
references: 15706611
references: Mass Spectrom Rev. 2006 Mar-Apr;25(2):173-214
references: 16284938
references: J Proteome Res. 2006 Apr;5(4):935-43
references: 16602701
references: Proteomics. 2005 Apr;5(5):1317-30
references: 15717325
references: Anal Chim Acta. 2006 Oct 10;579(2):158-76
references: 17723740
references: J Chromatogr B Analyt Technol Biomed Life Sci. 2006 Dec 5;844(2):204-12
references: 16935574
references: J Proteome Res. 2006 Oct;5(10):2632-41
references: 17022634
references: Plant Physiol. 2003 Jan;131(1):198-214
references: 12529528
references: Anal Chem. 2005 Apr 15;77(8):2587-94
references: 15828797
references: Methods Mol Biol. 2007;357:297-305
references: 17172695
references: Chem Res Toxicol. 2005 Jun;18(6):924-33
references: 15962927
references: J Dairy Res. 2006 Nov;73(4):406-16
references: 16834814
references: Nucleic Acids Res. 2006 Jun 28;34(11):3267-78
references: 16807317
references: Pharm Res. 2003 Nov;20(11):1713-20
references: 14661913
references: J Proteome Res. 2005 Mar-Apr;4(2):570-7
references: 15822936
references: Mol Cell Proteomics. 2004 Mar;3(3):257-65
references: 14724321
references: J Proteome Res. 2006 Nov;5(11):3197-204
references: 17081072
references: J Chromatogr B Analyt Technol Biomed Life Sci. 2007 Apr 15;849(1-2):81-90
references: 17113368
references: Proteomics. 2005 Nov;5(16):4145-51
references: 16196087
references: Anal Bioanal Chem. 2007 Oct;389(4):1055-64
references: 17221235
references: Biochem Pharmacol. 2006 May 28;71(11):1619-28
references: 16616895
references: J Neurochem. 2006 Sep;98(6):1763-77
references: 16903874
references: Anal Chem. 2004 Jan 1;76(1):144-54
references: 14697044
references: J Neurochem. 2006 Sep;98(6):1999-2012
references: 16945111
references: Int J Biochem Cell Biol. 2007;39(1):220-6
references: 17005433
references: J Proteome Res. 2007 Jun;6(6):2257-68
references: 17497906
references: Anal Chem. 2003 Jul 15;75(14):3411-8
references: 14570191
references: Mol Cell Proteomics. 2005 Oct;4(10):1503-21
references: 15985654
references: Biochem Biophys Res Commun. 2005 Sep 16;335(1):76-86
references: 16061202
references: J Proteome Res. 2006 Dec;5(12):3345-54
references: 17137336
references: J Proteome Res. 2003 Sep-Oct;2(5):506-13
references: 14582647
references: Anal Chem. 2000 Nov 1;72(21):5356-60
references: 11080887
references: Electrophoresis. 2002 Sep;23(18):3106-24
references: 12298083
references: Proteomics. 2005 Jul;5(11):2949-59
references: 15996010
references: J Proteome Res. 2005 Nov-Dec;4(6):2283-93
references: 16335977
references: Biochem Biophys Res Commun. 2006 Feb 17;340(3):909-15
references: 16386709
references: Proteomics. 2006 Mar;6(6):1976-88
references: 16475230
references: J Proteome Res. 2006 Jan;5(1):127-34
references: 16396503
references: J Proteome Res. 2004 Nov-Dec;3(6):1138-48
references: 15595722
references: Proteomics. 2007 Jan;7(1):121-9
references: 17205597
references: Anal Biochem. 1998 May 1;258(2):349-61
references: 9570851
references: J Proteome Res. 2007 Aug;6(8):3127-33
references: 17625814
references: J Proteome Res. 2004 Nov-Dec;3(6):1278-83
references: 15595738
references: NeuroRx. 2006 Jul;3(3):336-43
references: 16815217
references: Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai). 2001;33(5):477-482
references: 12040386
references: J Chromatogr B Analyt Technol Biomed Life Sci. 2005 Feb 5;815(1-2):51-63
references: 15652798
references: Chirality. 2005 Jun;17(6):332-7
references: 15856432