title: Highly specific and sensitive non-enzymatic determination of uric acid in serum and urine by extended gate field effect transistor sensors.
abstract: A potentiometric non-enzymatic sensor using off-chip extended-gate field effect transistor (EGFET) with a ferrocenyl-alkanethiol modified gold electrode is demonstrated for determining the uric acid concentration in human serum and urine. Hexacyanoferrate (II) and (III) ions are used as redox reagent. This potentiometric sensor measures the interface potential on the ferrocene immobilized gold electrode, which is modulated by the redox reaction between uric acid and hexacyanoferrate ions. The device shows a near Nernstian response to uric acid and is highly specific. The interference that comes from glucose, bilirubin, ascorbic acid and hemoglobin is negligible in normal concentration range of these interferents. The sensor also exhibits excellent long term reliability. This extended gate field effect transistor based sensors can be used as a point of care UA testing tool, due to the small size, low cost, and low sample volume consumption.
authors: Department of Electrical Engineering, Yale University, New Haven, CT 06520, USA. Electronic address: weihua.guan@yale.edu.
authors: Weihua
authors: W
authors: Guan
authors: Xuexin
authors: X
authors: Duan
authors: Mark A
authors: MA
authors: Reed
mesh_terms: D015374:Biosensing Techniques / Q000191:economics / Q000295:instrumentation*; D004566:Electrodes; D005295:Ferrocyanides / Q000737:chemistry*; D005296:Ferrous Compounds / Q000737:chemistry*; D006046:Gold / Q000737:chemistry; D006801:Humans; D057230:Limit of Detection; D000075163:Metallocenes; D011199:Potentiometry / Q000191:economics / Q000295:instrumentation; D015203:Reproducibility of Results; D014173:Transistors, Electronic; D014527:Uric Acid / Q000097:blood* / Q000652:urine*
chemical_list: D005295:Ferrocyanides; D005296:Ferrous Compounds; D000075163:Metallocenes; C116383:ferrocyn; D014527:Uric Acid; D006046:Gold; C004998:ferrocene
keywords: Extended gate FET; Ferrocenyl-alkanethiol; Non-enzymatic; Potentiometric sensor; Uric acid sensor