title: Comparing the genomes of Helicobacter pylori clinical strain UM032 and Mice-adapted derivatives.
abstract: BACKGROUND
  Helicobacter pylori is a Gram-negative bacterium that persistently infects the human stomach inducing chronic inflammation. The exact mechanisms of pathogenesis are still not completely understood. Although not a natural host for H. pylori, mouse infection models play an important role in establishing the immunology and pathogenicity of H. pylori. In this study, for the first time, the genome sequences of clinical H. pylori strain UM032 and mice-adapted derivatives, 298 and 299, were sequenced using the PacBio Single Molecule, Real-Time (SMRT) technology.
  
  
  RESULTS
  Here, we described the single contig which was achieved for UM032 (1,599,441 bp), 298 (1,604,216 bp) and 299 (1,601,149 bp). Preliminary analysis suggested that methylation of H. pylori genome through its restriction modification system may be determinative of its host specificity and adaptation.
  
  
  CONCLUSIONS
  Availability of these genomic sequences will aid in enhancing our current level of understanding the host specificity of H. pylori.
authors: Department of Medical Microbiology, University of Malaya, Kuala Lumpur, Malaysia.
authors: Yalda
authors: Y
authors: Khosravi
authors: Department of Medical Microbiology, University of Malaya, Kuala Lumpur, Malaysia.
authors: Vellaya
authors: V
authors: Rehvathy
authors: Dental Research and Training Unit, Faculty of Dentistry, University of Malaya, Kuala Lumpur, Malaysia.
authors: Wei Yee
authors: WY
authors: Wee
authors: Pacific Biosciences, Menlo Park, California, USA.
authors: Susana
authors: S
authors: Wang
authors: Pacific Biosciences, Menlo Park, California, USA.
authors: Primo
authors: P
authors: Baybayan
authors: PacBio Singapore, Singapore, Singapore.
authors: Siddarth
authors: S
authors: Singh
authors: Pacific Biosciences, Menlo Park, California, USA.
authors: Meredith
authors: M
authors: Ashby
authors: National Cancer Centre, Singapore, Singapore.
authors: Junxian
authors: J
authors: Ong
authors: National Cancer Centre, Singapore, Singapore.
authors: Arlaine Anne
authors: AA
authors: Amoyo
authors: National Cancer Centre, Singapore, Singapore.
authors: Shih Wee
authors: SW
authors: Seow
authors: Dental Research and Training Unit, Faculty of Dentistry, University of Malaya, Kuala Lumpur, Malaysia.
authors: Siew Woh
authors: SW
authors: Choo
authors: School of Pathology and Laboratory Medicine, Faculty of Medicine, Dentistry and Health Sciences, University of Western Australia, Perth, Western Australia, Australia.
authors: Tim
authors: T
authors: Perkins
authors: The Marshall Centre for Infectious Diseases Research and Training, University of Western Australia, Perth, Western Australia, Australia.
authors: Eng Guan
authors: EG
authors: Chua
authors: The Marshall Centre for Infectious Diseases Research and Training, University of Western Australia, Perth, Western Australia, Australia.
authors: Alfred
authors: A
authors: Tay
authors: The Marshall Centre for Infectious Diseases Research and Training, University of Western Australia, Perth, Western Australia, Australia.
authors: Barry James
authors: BJ
authors: Marshall
authors: Department of Medical Microbiology, University of Malaya, Kuala Lumpur, Malaysia.
authors: Mun Fai
authors: MF
authors: Loke
authors: Department of Medicine, University of Malaya, Kuala Lumpur, Malaysia.
authors: Khean Lee
authors: KL
authors: Goh
authors: National Cancer Centre, Singapore, Singapore ; Department of Microbiology, Tumor and Cell Biology (MTC), Karolinska Institutet, Stockholm, Sweden ; School of Biological Sciences, Nanyang Technological University, Singapore, Singapore.
authors: Sven
authors: S
authors: Pettersson
authors: Department of Medical Microbiology, University of Malaya, Kuala Lumpur, Malaysia.
authors: Jamuna
authors: J
authors: Vadivelu
keywords: Clinical H. pylori; Helicobacter pylori; Mice-adapted; PacBio Single Molecule; Real-Time (SMRT) technology
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