title: Detection of high-risk human papillomaviruses in fresh breast cancer samples using the hybrid capture 2 assay.
abstract: The etiology of breast cancer remains unknown and the role of human papillomavirus (HPV) in breast carcinogenesis is controversial. This study investigated the prevalence of high-risk HPV infections in Chinese women with breast cancer and the possible relationship between high-risk HPV infection and the clinicopathological characteristics of breast cancer. Tumor cells from 224 fresh breast cancer samples and 37 fresh breast fibroadenomas were collected for hybrid capture 2 (HC2) assay. HC2 was the only technique approved by the United States Food and Drug Administration for screening for high-risk HPV infection in 2008. The prevalence of high-risk HPV infection in breast cancer samples was 21.4%, which was slightly higher than the 16.2% observed in breast fibroadenomas. Age and menopausal status were not risk factors for high-risk HPV infection among breast cancer patients. The clinical and pathological characteristics of breast cancer showed no significant correlation with high-risk HPV infection. Although the prevalence of 13 subtypes of high-risk HPV infections was similar in breast cancer and nonmalignant breast samples, the presence of high-risk HPVs in both malignant and benign breast samples implies that a possible causal role in breast cancer carcinogenesis could not be ruled out. Clarifying the possible link between high-risk HPVs and breast cancer might benefit women vaccinated against HPV and could decrease the incidence of HPV-related breast cancer.
authors: Department of Breast Surgery, Qilu Hospital of Shandong University, Shandong, P.R., China.
authors: Weili
authors: W
authors: Liang
authors: Jianli
authors: J
authors: Wang
authors: Chongjie
authors: C
authors: Wang
authors: Yanrong
authors: Y
authors: Lv
authors: Haidong
authors: H
authors: Gao
authors: Kai
authors: K
authors: Zhang
authors: Huantao
authors: H
authors: Liu
authors: Jinbo
authors: J
authors: Feng
authors: Leiyi
authors: L
authors: Wang
authors: Rong
authors: R
authors: Ma
mesh_terms: D000328:Adult; D000368:Aged; D001943:Breast Neoplasms / Q000453:epidemiology / Q000473:pathology / Q000821:virology*; D005260:Female; D006801:Humans; D008875:Middle Aged; D009362:Neoplasm Metastasis; D009367:Neoplasm Staging; D027383:Papillomaviridae / Q000145:classification / Q000235:genetics*; D030361:Papillomavirus Infections / Q000150:complications / Q000175:diagnosis / Q000821:virology*; D015995:Prevalence
keywords: breast carcinoma; human papillomavirus; hybrid capture 2