title: Prediction and design of outer membrane protein-protein interactions.
abstract: Protein-protein interactions (PPI) play central roles in biological processes, motivating us to understand the structural basis underlying affinity and specificity. In this chapter, we focus on biochemical and computational design strategies of assessing and detecting PPIs of β-barrel outer membrane proteins (OMPs). A few case studies are presented highlighting biochemical techniques used to dissect the energetics of oligomerization and determine amino acids forming the key interactions of the PPI sites. Current computational strategies for detecting/predicting PPIs are introduced, and examples of computational and rational engineering strategies applied to OMPs are presented.
authors: Department of Biochemistry and Molecular Biology, Robert Wood Johnson Medical School-UMDNJ, Piscataway, NJ, USA.
authors: Vikas
authors: V
authors: Nanda
authors: Daniel
authors: D
authors: Hsieh
authors: Alexander
authors: A
authors: Davis
mesh_terms: D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D015202:Protein Engineering; D025941:Protein Interaction Mapping / Q000379:methods*; D055503:Protein Multimerization; D020836:Protein Structure, Quaternary
chemical_list: D001425:Bacterial Outer Membrane Proteins