title: Coating barium titanate nanoparticles with polyethylenimine improves cellular uptake and allows for coupled imaging and gene delivery.
abstract: Barium titanate nanoparticles (BT NP) belong to a class of second harmonic generating (SHG) nanoprobes that have recently demonstrated promise in biological imaging. Unfortunately, BT NPs display low cellular uptake efficiencies, which may be a problem if cellular internalization is desired or required for a particular application. To overcome this issue, while concomitantly developing a particle platform that can also deliver nucleic acids into cells, we coated the BT NPs with the cationic polymer polyethylenimine (PEI)-one of the most effective nonviral gene delivery agents. Coating of BT with PEI yielded complexes with positive zeta potentials and resulted in an 8-fold increase in cellular uptake of the BT NPs. Importantly, we were able to achieve high levels of gene delivery with the BT-PEI/DNA complexes, supporting further efforts to generate BT platforms for coupled imaging and gene therapy.
authors: Rice University, George R. Brown School of Engineering, Department of Bioengineering, Houston, TX, United States.
authors: Christopher
authors: C
authors: Dempsey
authors: Isac
authors: I
authors: Lee
authors: Katie R
authors: KR
authors: Cowan
authors: Junghae
authors: J
authors: Suh
mesh_terms: D017609:Barium Compounds / Q000737:chemistry*; D001693:Biological Transport, Active; D002470:Cell Survival; D004274:DNA, Recombinant / Q000008:administration & dosage / Q000235:genetics; D004337:Drug Carriers; D018014:Gene Transfer Techniques; D015316:Genetic Therapy; D049452:Green Fluorescent Proteins / Q000235:genetics; D006367:HeLa Cells; D006801:Humans; D053768:Metal Nanoparticles / Q000008:administration & dosage / Q000737:chemistry* / Q000648:ultrastructure; D018613:Microscopy, Confocal; D057054:Molecular Imaging / Q000379:methods; D011094:Polyethyleneimine / Q000737:chemistry*; D014025:Titanium / Q000737:chemistry*; D014162:Transfection
chemical_list: D017609:Barium Compounds; D004274:DNA, Recombinant; D004337:Drug Carriers; C024547:barium titanate(IV); D049452:Green Fluorescent Proteins; D011094:Polyethyleneimine; D014025:Titanium
keywords: Barium titanate; Polyethylenimine; Second harmonic generation; Transfection
references: Anal Chem. 2012 Nov 6;84(21):9238-45
references: 22967239
references: Biotechnol Bioeng. 2002 Jun 5;78(5):503-8
references: 12115119
references: Colloids Surf B Biointerfaces. 2010 Apr 1;76(2):535-43
references: 20060274
references: Chem Commun (Camb). 2013 Jan 21;49(6):549-51
references: 23192002
references: Proc Natl Acad Sci U S A. 1995 Aug 1;92(16):7297-301
references: 7638184
references: Nanotechnology. 2008 Sep 24;19(38):384015
references: 21832574
references: Nanomedicine (Lond). 2011 Jun;6(4):693-700
references: 21718178
references: Nanoscale Res Lett. 2010 May 09;5(7):1093-101
references: 20596329
references: Opt Lett. 2003 Jun 15;28(12):1022-4
references: 12836766
references: Proc Natl Acad Sci U S A. 2010 Aug 17;107(33):14535-40
references: 20668245
references: Proc Natl Acad Sci U S A. 2003 Apr 1;100(7):3878-82
references: 12644705
references: Bioconjug Chem. 2008 Mar;19(3):740-50
references: 18288791
references: ACS Nano. 2009 Oct 27;3(10):3273-86
references: 19739605
references: Biomaterials. 2010 May;31(13):3657-66
references: 20138662
references: ACS Nano. 2008 Jul;2(7):1403-10
references: 19206308
references: Nano Lett. 2004 Jan 1;4(1):11-18
references: 28890669
references: Biomaterials. 2008 Apr;29(10):1526-32
references: 18096220
references: J Control Release. 2002 Sep 18;83(1):157-68
references: 12220847
references: Int J Pharm. 2002 Feb 21;233(1-2):51-9
references: 11897410
references: Adv Funct Mater. 2009 Jul 24;19(14):2244-2251
references: 20160995
references: Biomacromolecules. 2010 Jun 14;11(6):1437-42
references: 20426406
references: Angew Chem Int Ed Engl. 2008;47(28):5163-6
references: 18528828
references: Macromol Biosci. 2008 Dec 8;8(12):1135-43
references: 18698581
references: Eur J Pharm Biopharm. 2008 Jan;68(1):129-37
references: 17881203
references: Opt Express. 2009 Feb 16;17(4):2880-91
references: 19219192