title: Genome analysis of Desulfotomaculum kuznetsovii strain 17(T) reveals a physiological similarity with Pelotomaculum thermopropionicum strain SI(T).
abstract: Desulfotomaculum kuznetsovii is a moderately thermophilic member of the polyphyletic spore-forming genus Desulfotomaculum in the family Peptococcaceae. This species is of interest because it originates from deep subsurface thermal mineral water at a depth of about 3,000 m. D. kuznetsovii is a rather versatile bacterium as it can grow with a large variety of organic substrates, including short-chain and long-chain fatty acids, which are degraded completely to carbon dioxide coupled to the reduction of sulfate. It can grow methylotrophically with methanol and sulfate and autotrophically with H2 + CO2 and sulfate. For growth it does not require any vitamins. Here, we describe the features of D. kuznetsovii together with the genome sequence and annotation. The chromosome has 3,601,386 bp organized in one contig. A total of 3,567 candidate protein-encoding genes and 58 RNA genes were identified. Genes of the acetyl-CoA pathway, possibly involved in heterotrophic growth with acetate and methanol, and in CO2 fixation during autotrophic growth are present. Genomic comparison revealed that D. kuznetsovii shows a high similarity with Pelotomaculum thermopropionicum. Genes involved in propionate metabolism of these two strains show a strong similarity. However, main differences are found in genes involved in the electron acceptor metabolism.
authors: Wageningen University, Laboratory of Microbiology, Wageningen, the Netherlands.
authors: Michael
authors: M
authors: Visser
authors: Petra
authors: P
authors: Worm
authors: Gerard
authors: G
authors: Muyzer
authors: Inês A C
authors: IA
authors: Pereira
authors: Peter J
authors: PJ
authors: Schaap
authors: Caroline M
authors: CM
authors: Plugge
authors: Jan
authors: J
authors: Kuever
authors: Sofiya N
authors: SN
authors: Parshina
authors: Tamara N
authors: TN
authors: Nazina
authors: Anna E
authors: AE
authors: Ivanova
authors: Rizlan
authors: R
authors: Bernier-Latmani
authors: Lynne A
authors: LA
authors: Goodwin
authors: Nikos C
authors: NC
authors: Kyrpides
authors: Tanja
authors: T
authors: Woyke
authors: Patrick
authors: P
authors: Chain
authors: Karen W
authors: KW
authors: Davenport
authors: Stefan
authors: S
authors: Spring
authors: Hans-Peter
authors: HP
authors: Klenk
authors: Alfons J M
authors: AJ
authors: Stams
keywords: Clostridiales; Peptococcaceae; Thermophilic spore-forming anaerobes; autotrophic; methylotrophic; sulfate reduction
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