title: Two-dimensional surface plasmon resonance imager: An approach to study neuronal differentiation.
abstract: There is a growing demand for the development of a new bioanalytical technique that is capable of monitoring neuronal differentiation noninvasively, in real time, and without any fluorescent probes. In a previous article, we demonstrated that a high-resolution two-dimensional surface plasmon resonance (2D-SPR) imager was very useful to monitor cell response on chemical stimulation in which protein kinase C (PKC) translocation was related. In the current study, we focused on developing a new method for monitoring neuronal differentiation and examined the application of the high-resolution 2D-SPR imager to monitor neuronal differentiation noninvasively and by a label-free format. We successfully monitored the intracellular signal transduction, which was mainly translocation of PKC in PC12 cells by the 2D-SPR imager, and found that the cells treated with a differentiation factor, nerve growth factor (NGF), showed a remarkable enhancement of 2D-SPR response to muscarine, carbachol, and acetylcholine stimulation. The results demonstrated that 2D-SPR sensing is applicable to in situ assessment of neuronal differentiation and to studying the expression state of the specific receptors in the living state.
authors: Biological Information Systems Science, Graduate School of Innovative Life Science for Education, University of Toyama, Toyama 930-8555, Japan. Electronic address: mirtanveer0699@gmail.com.
authors: Tanveer Ahamd
authors: TA
authors: Mir
authors: Hiroaki
authors: H
authors: Shinohara
mesh_terms: D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D002217:Carbachol / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects; D015870:Gene Expression / Q000187:drug effects; D057054:Molecular Imaging; D009116:Muscarine / Q000494:pharmacology*; D009414:Nerve Growth Factors / Q000494:pharmacology*; D009474:Neurons / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D016716:PC12 Cells; D051381:Rats; D011950:Receptors, Cholinergic / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D020349:Surface Plasmon Resonance
chemical_list: D009414:Nerve Growth Factors; D011950:Receptors, Cholinergic; D009116:Muscarine; D002217:Carbachol; D000109:Acetylcholine
keywords: 2D–SPRI; NGF; Neuronal differentiation; PC12 cell