title: Validation of an rpoB gene PCR assay for detection of Tropheryma whipplei: 10 years' experience in a National Reference Laboratory.
abstract: The performance of a real-time PCR assay targeting the Tropheryma whipplei rpoB gene was evaluated using test strains and 1,236 clinical specimens in a national reference laboratory. The novel rpoB-PCR assay proved to be specific, revealed improved analytical sensitivity, and substantially accelerated detection of T. whipplei DNA in clinical specimens.
authors: Institut für Mikrobiologie und Hygiene, Charité-Universitätsmedizin Berlin, Berlin, Germany.
authors: Annette
authors: A
authors: Moter
authors: Dinah
authors: D
authors: Schmiedel
authors: Annett
authors: A
authors: Petrich
authors: Alexandra
authors: A
authors: Wiessner
authors: Judith
authors: J
authors: Kikhney
authors: Thomas
authors: T
authors: Schneider
authors: Verena
authors: V
authors: Moos
authors: Ulf B
authors: UB
authors: Göbel
authors: Udo
authors: U
authors: Reischl
mesh_terms: D001431:Bacteriological Techniques / Q000379:methods*; D012321:DNA-Directed RNA Polymerases / Q000235:genetics*; D006801:Humans; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D013997:Time Factors; D054851:Tropheryma / Q000235:genetics / Q000302:isolation & purification*; D008061:Whipple Disease / Q000175:diagnosis* / Q000382:microbiology
chemical_list: D012321:DNA-Directed RNA Polymerases; C105078:RNA polymerase beta subunit
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