title: Optimizations to achieve high-level expression of cytochrome P450 proteins using Escherichia coli expression systems.
abstract: Recombinant expression of membrane-bound cytochrome P450s in bacterial expression systems provide a well-established system capable of producing large yields of catalytically active protein. As the biochemical knowledge regarding cytochrome P450s increases, so does the efficiency of protein expression through various modifications that do not disrupt the functional properties of the protein of interest. Changes such as N-terminal modifications, reduction of secondary mRNA structure, bacterial codon usage, selection of vector and host strain, as well as varying external growth conditions all appear to influence protein expression. Several optimizations are often required for sufficient expression of cytochrome P450s at the desired cellular localization. This review aims to comprehensively summarize and update the significant advances made in membrane protein P450 expression in bacterial expression systems.
authors: Department of Comparative Biosciences, University of Illinois Urbana-Champaign, Urbana, IL 61801, USA.
authors: Susan
authors: S
authors: Zelasko
authors: Amrita
authors: A
authors: Palaria
authors: Aditi
authors: A
authors: Das
mesh_terms: D003001:Cloning, Molecular; D003062:Codon / Q000235:genetics; D003577:Cytochrome P-450 Enzyme System / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics*; D015870:Gene Expression; D006801:Humans; D008958:Models, Molecular; D009154:Mutation; D011487:Protein Conformation; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
chemical_list: D003062:Codon; D011994:Recombinant Proteins; D003577:Cytochrome P-450 Enzyme System
keywords: Cytochrome P450 protein; Cytochrome P450s; Expression systems; N-terminus modifications; Recombinant expression; Silent mutations