title: Targeted mutagenesis in the malaria mosquito using TALE nucleases.
abstract: Anopheles gambiae, the main mosquito vector of human malaria, is a challenging organism to manipulate genetically. As a consequence, reverse genetics studies in this disease vector have been largely limited to RNA interference experiments. Here, we report the targeted disruption of the immunity gene TEP1 using transgenic expression of Transcription-Activator Like Effector Nucleases (TALENs), and the isolation of several TEP1 mutant A. gambiae lines. These mutations inhibited protein production and rendered TEP1 mutants hypersusceptible to Plasmodium berghei. The TALEN technology opens up new avenues for genetic analysis in this disease vector and may offer novel biotechnology-based approaches for malaria control.
authors: Institut National de la Santé et de la Recherche Médicale U963, Strasbourg, France.
authors: Andrea L
authors: AL
authors: Smidler
authors: Olivier
authors: O
authors: Terenzi
authors: Julien
authors: J
authors: Soichot
authors: Elena A
authors: EA
authors: Levashina
authors: Eric
authors: E
authors: Marois
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000852:Anopheles / Q000235:genetics* / Q000469:parasitology*; D001483:Base Sequence; D004720:Endonucleases / Q000378:metabolism*; D018390:Gene Targeting; D017344:Genes, Insect; D006801:Humans; D019476:Insect Proteins / Q000737:chemistry / Q000235:genetics; D008288:Malaria / Q000469:parasitology*; D008969:Molecular Sequence Data; D016296:Mutagenesis / Q000235:genetics*; D050505:Mutant Proteins / Q000737:chemistry / Q000235:genetics; D009154:Mutation / Q000235:genetics; D010962:Plasmodium berghei / Q000502:physiology; D015534:Trans-Activators / Q000378:metabolism*
chemical_list: D019476:Insect Proteins; D050505:Mutant Proteins; C544265:TEP1 protein, Anopheles gambiae; D015534:Trans-Activators; D004720:Endonucleases
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