title: Antifungal susceptibilities and identification of species of the Sporothrix schenckii complex isolated in Brazil.
abstract: Sporotrichosis is a subacute or chronic mycosis caused worldwide by the dimorphic species complex, Sporothrix schenckii. We studied 85 isolates recovered in Brazil to verify their identification and evaluate their in vitro antifungal susceptibility patterns. Based on phenotypic tests (microscopic features, ability to grow at 30°C and 37°C, colony diameters, as well as assimilation of sucrose and raffinose) and molecular assays (amplification of a fragment of the calmodulin gene), the strains were identified as S. schenckii, S. brasiliensis and S. globosa, with a predominance of S. schenckii isolates. There was 37.7% disagreement between the phenotypic and genotypic identification methodologies. In general, terbinafine was the most active drug, followed by ketoconazole and itraconazole, and the less active fluconazole and voriconazole. Five isolates (one S. globosa and four S. schenckii) were found to be itraconazole-resistant strains but, in general, there were no differences in the in vitro antifungal susceptibility profiles among the Sporothrix species.
authors: Programa de Pós-Graduação em Medicina: Ciências Médicas, Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil.
authors: Cheila Denise
authors: CD
authors: Ottonelli Stopiglia
authors: Cibele Massotti
authors: CM
authors: Magagnin
authors: Mauricio Ramírez
authors: MR
authors: Castrillón
authors: Sandra Denise Camargo
authors: SD
authors: Mendes
authors: Daiane
authors: D
authors: Heidrich
authors: Patricia
authors: P
authors: Valente
authors: Maria Lúcia
authors: ML
authors: Scroferneker
mesh_terms: D000935:Antifungal Agents / Q000494:pharmacology*; D001938:Brazil; D006801:Humans; D008826:Microbial Sensitivity Tests; D058889:Molecular Typing; D016533:Mycological Typing Techniques; D013175:Sporothrix / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology; D013174:Sporotrichosis / Q000382:microbiology*
chemical_list: D000935:Antifungal Agents
keywords: Sporothrix; antifungal agents; sporotrichosis