title: Doxorubicin-chitin-poly(caprolactone) composite nanogel for drug delivery.
abstract: In this work, we developed a pH responsive chitin-poly(caprolactone) composite nanogels (chitin-PCL CNGs) system for non-small cell lung cancer (NSCLC). A hydrophilic drug, doxorubicin (Dox) was loaded in Chitin-PCL CNGs (Dox-chitin-PCL CNGs). Both control and drug loaded systems were analyzed by DLS, SEM, FTIR and TG/DTA. The size ranges of the control composite nanogels and their drug loaded counterparts were found to be 70 ± 20 and 240 ± 20 nm, respectively. The control chitin-PCL CNGs and Dox-chitin-PCL CNGs showed higher swelling and degradation in acidic pH. Drug entrapment efficiency and in-vitro drug release studies were carried out and showed a higher drug release at acidic pH compared to neutral pH. Cellular internalization of the nanogel systems was confirmed by fluorescent microscopy. Dox-Chitin-PCL CNGs showed dose dependent cytotoxicity toward A549 (adenocarcinomic human alveolar basal epithelial cells) cancer cells. Furthermore, the results of in-vitro hemolytic assay and coagulation assay substantiate the blood compatibility of the system. These results indicate that chitin-PCL CNGs is a novel carrier for delivery of anticancer drugs.
authors: Amrita Centre for Nanosciences and Molecular Medicine, Amrita Institute of Medical Sciences and Research Centre, Amrita Vishwa Vidyapeetham University, Kochi 682041, India.
authors: T R
authors: TR
authors: Arunraj
authors: N
authors: N
authors: Sanoj Rejinold
authors: N
authors: N
authors: Ashwin Kumar
authors: R
authors: R
authors: Jayakumar
mesh_terms: D000818:Animals; D001692:Biological Transport; D045744:Cell Line, Tumor; D055598:Chemical Phenomena; D002686:Chitin / Q000737:chemistry*; D004317:Doxorubicin / Q000737:chemistry*; D004337:Drug Carriers / Q000737:chemistry* / Q000378:metabolism / Q000633:toxicity; D065546:Drug Liberation; D005782:Gels; D006801:Humans; D008422:Materials Testing; D049329:Nanostructures / Q000737:chemistry*; D011091:Polyesters / Q000737:chemistry*
chemical_list: D004337:Drug Carriers; D005782:Gels; D011091:Polyesters; D002686:Chitin; C016240:polycaprolactone; D004317:Doxorubicin
keywords: Cancer nanomedicine; Chitin; Composite nanogels; Drug delivery; Lung cancer; PCL