title: Lead acetate may cause erectile dysfunction by modulating NO/cGMP pathway in rat corpus cavernosum.
abstract: Despite the fact that metal toxicity has been widely reported in industrial toxicological studies, very little has been reported about the effect of lead exposure on erectile function. This study investigated the effect of lead on erectile function in rats and aimed to preliminarily test the mechanisms by which it might affect erection. Rats were injected with lead acetate (0.25-2 mg/kg) intraperitoneally for 21 days. Intracavernosal pressure/mean arterial pressure (ICP/MAP) next to nerve stimulation; nitrite/nitrate; malonaldehyde; and reduced glutathione levels and superoxide dismutase activity in the corpus cavernosum, kidney, and brain were measured in addition to creatinine, urea, and testosterone. For acute studies, rats were injected intravenously with lead acetate, and then ICP/MAP was recorded for 45 min. Subacute treatment significantly reduced erection with significant elevation of malonaldehyde and reduction of nitrite/nitrate levels in the corpus cavernosum. In acute studies, lead (2 and 5 mg/kg) reduced neurogenic erections by 28.42 ± 3.76 and 96.84 ± 8.52%, respectively, an effect that was masked in the presence of NG-nitro-L-arginine, tetraethyl ammonium, or methylene blue, but not zinc protoporphyrine, and reversed by vitamin C and partially by sildenafil. Lead acetate may inhibit the erectile process in rats. Besides its prooxidant effect and consequent inactivation of nitric oxide, lead may negatively modulate the action of nitric oxide on guanylate cyclase and potassium channels.
authors: Department of Pharmacology and Toxicology, Faculty of Pharmacy, Alexandria University, Azarita, P. Box: 21521, Alexandria, Egypt, senbelamira@yahoo.com.
authors: Amira M
authors: AM
authors: Senbel
authors: Mai M
authors: MM
authors: Helmy
mesh_terms: D000818:Animals; D001205:Ascorbic Acid / Q000494:pharmacology; D006152:Cyclic GMP / Q000378:metabolism*; D004785:Environmental Pollutants / Q000633:toxicity*; D007172:Erectile Dysfunction / Q000139:chemically induced*; D007668:Kidney / Q000187:drug effects / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism*; D009942:Organometallic Compounds / Q000633:toxicity*; D018384:Oxidative Stress; D010879:Piperazines / Q000494:pharmacology; D011687:Purines / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D015398:Signal Transduction / Q000187:drug effects*; D000068677:Sildenafil Citrate; D013450:Sulfones / Q000494:pharmacology; D064804:Urological Agents / Q000494:pharmacology
chemical_list: D004785:Environmental Pollutants; D009942:Organometallic Compounds; D010879:Piperazines; D011687:Purines; D013450:Sulfones; D064804:Urological Agents; D009569:Nitric Oxide; D008315:Malondialdehyde; D000068677:Sildenafil Citrate; D006152:Cyclic GMP; D001205:Ascorbic Acid; C008261:lead acetate