title: Seroepidemiological survey for canine angiostrongylosis in dogs from Germany and the UK using combined detection of Angiostrongylus vasorum antigen and specific antibodies.
abstract: Dogs infected with Angiostrongylus vasorum, a potentially lethal parasite parasitizing the heart and pulmonary arteries, may present severe respiratory, haematological and neurological signs. In this first large-scale seroepidemiological survey, 4003 sera originating from Germany and 4030 from the UK were tested by an ELISA for the detection of circulating antigen of A. vasorum, and by a separate ELISA detecting specific antibodies. In Germany, where mainly western federal states were sampled, 0·3% (n = 13, CI: 0·2–0·6%) of dogs were positive in both ELISAs, whereas in total 0·5% (n = 20, CI: 0·3–0·8%) were antigen-positive and 2·25% (n = 90, CI: 1·8–2·8%) were positive for specific antibodies. Regions with antigen- and antibody-positive animals were overlapping. In the UK, where mainly the south of the country was sampled, 0·97% (n = 39, CI: 0·7–1·3%) of dogs were antigen- and antibody positive. In total, 1·32% (n = 53, CI: 1·0–1·7%) were antigen-positive, and 3·2% (n = 129, CI: 2·7–3·8%) were positive for specific antibodies, again in overlapping regions. These results confirm the occurrence of A. vasorum in a random dog population originating from large parts of the countries investigated. The use of the tests alone or in combination was considered as a function of their sensitivities and specificities, in order to guide efficient clinical and epidemiological application.
authors: Vetsuisse Faculty, Institute of Parasitology, University of Zurich, Winterthurerstrasse 266a, 8057 Zürich, Switzerland. manuela.schnyder@uzh.ch
authors: M
authors: M
authors: Schnyder
authors: R
authors: R
authors: Schaper
authors: G
authors: G
authors: Bilbrough
authors: E R
authors: ER
authors: Morgan
authors: P
authors: P
authors: Deplazes
mesh_terms: D000801:Angiostrongylus / Q000276:immunology* / Q000302:isolation & purification; D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood*; D000947:Antigens, Helminth / Q000097:blood*; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005858:Germany / Q000453:epidemiology; D012680:Sensitivity and Specificity; D016036:Seroepidemiologic Studies; D017206:Strongylida Infections / Q000453:epidemiology / Q000469:parasitology / Q000662:veterinary*; D006113:United Kingdom / Q000453:epidemiology
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth
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