title: Size tuning of Ag-decorated TiO₂ nanotube arrays for improved bactericidal capacity of orthopedic implants.
abstract: Surface modification of orthopedic implants using titanium dioxide nanotubes and silver nanoparticles (SNs) is a promising approach to prevent bacteria adhesion, biofilm formation, and implant infection. Herein, we utilized a straightforward and all-solution process to prepare silver-decorated TiO2 nanotube arrays with surface density of 10(3) to 10(4) per µm(2). With controlling the synthesis conditions, hexagonal closed-packed nanotubes with opening diameter of 30-100 nm that are decorated with SNs with varying sizes (12-40 nm) were prepared. Various analytical techniques were utilized to characterize the size, morphology, distribution, valance state, surface roughness, and composition of the prepared antibacterial films. The bactericidal capacity of the films were studied on Escherichia coli (E. coli) by drop-test method and correlated with the size and percentage of Ag as well as the surface density of TiO2 nanotube arrays. Synergetic effect of TiO2 nanotubes and SNs on the antibacterial activity of the composite films is shown. The bactericidal capacity is found to depend on the size characteristics of the Ag-TiO2 coating. The highest antibacterial activity is obtained for TiO2 nanotubes with opening diameter of about 100 nm and SNs with an average size of 20 nm. MTT assay using osteoblast MG63 cells was performed to examine the cell viability. We suggest that release rate of the silver ions is an important factor controlling the antibacterial activity. Additionally, the size dependency of the bactericidal capacity implies that electrical coupling between silver and TiO2 nanotubes and improved hydrophobicity of the coating might influence the bacterial behavior of the hybrid nanostructures.
authors: Department of Materials Science and Engineering, Sharif University of Technology, P.O. Box 11365-9466, Tehran, Iran.
authors: N
authors: N
authors: Esfandiari
authors: A
authors: A
authors: Simchi
authors: R
authors: R
authors: Bagheri
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001842:Bone and Bones / Q000187:drug effects*; D015169:Colony Count, Microbial; D004926:Escherichia coli / Q000187:drug effects / Q000254:growth & development; D007477:Ions; D008826:Microbial Sensitivity Tests; D050296:Microbial Viability / Q000187:drug effects; D043942:Nanotubes / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size; D019736:Prostheses and Implants; D012834:Silver / Q000494:pharmacology*; D012996:Solutions; D014025:Titanium / Q000494:pharmacology*; D014961:X-Ray Diffraction
chemical_list: D000900:Anti-Bacterial Agents; D007477:Ions; D012996:Solutions; C009495:titanium dioxide; D012834:Silver; D014025:Titanium
keywords: E. coli; TiO2; antibacterial; nanotube; orthopedic implant; silver