title: Cytomegalovirus contributes partly to uraemia-associated premature immunological ageing of the T cell compartment.
abstract: Cytomegalovirus (CMV) infection has been implicated in accelerated T cell ageing. End-stage renal disease (ESRD) patients have a severely immunologically aged T cell compartment but also a high prevalence of CMV infection. We investigated whether CMV infection contributes to T cell ageing in ESRD patients. We determined the thymic output by the T cell receptor excision circle (TREC) content and percentage of CD31+ naïve T cells. The proliferative history of the T cell compartment by determination of the relative telomere length (RTL) and the T cell differentiation status was determined by immunophenotyping. It appeared that CMV infection did not affect thymic output but reduced RTL of CD8+ T cells in ESRD patients. Moreover, increased T cell differentiation was observed with higher percentages of CD57+ and CD28null CD4+ and CD8+ memory T cells. These CD28null T cells had significantly shorter telomeres compared to CD28+ T cells. Therefore we concluded that CMV infection does not affect the decreased thymic output but increases T cell differentiation as observed in ESRD-related premature T cell ageing.
authors: Department of Internal Medicine, Section Nephrology and Transplantation, Erasmus Medical Center, Rotterdam, the Netherlands.
authors: R W J
authors: RW
authors: Meijers
authors: N H R
authors: NH
authors: Litjens
authors: E A
authors: EA
authors: de Wit
authors: A W
authors: AW
authors: Langerak
authors: A
authors: A
authors: van der Spek
authors: C C
authors: CC
authors: Baan
authors: W
authors: W
authors: Weimar
authors: M G H
authors: MG
authors: Betjes
mesh_terms: D000328:Adult; D000375:Aging / Q000276:immunology*; D018106:CD28 Antigens / Q000378:metabolism; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology* / Q000378:metabolism; D018959:CD57 Antigens / Q000378:metabolism; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology* / Q000378:metabolism; D002454:Cell Differentiation / Q000276:immunology; D049109:Cell Proliferation; D003587:Cytomegalovirus / Q000276:immunology; D003586:Cytomegalovirus Infections / Q000276:immunology*; D005260:Female; D006801:Humans; D016130:Immunophenotyping; D019394:Ki-67 Antigen / Q000378:metabolism; D007676:Kidney Failure, Chronic / Q000276:immunology* / Q000821:virology; D008297:Male; D008875:Middle Aged; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000276:immunology; D019098:Telomerase / Q000378:metabolism; D016615:Telomere / Q000235:genetics; D059505:Telomere Homeostasis / Q000235:genetics; D014511:Uremia / Q000378:metabolism / Q000821:virology
chemical_list: D018106:CD28 Antigens; D018959:CD57 Antigens; D019394:Ki-67 Antigen; D019408:Platelet Endothelial Cell Adhesion Molecule-1; D019098:Telomerase
keywords: T lymphocytes; ageing; cytomegalovirus; end-stage renal disease; telomeres
references: Curr Opin Immunol. 2009 Aug;21(4):440-5
references: 19535233
references: Kidney Int. 2011 Jul;80(2):208-17
references: 21525849
references: J Immunol. 2002 Aug 15;169(4):1984-92
references: 12165524
references: J Immunol. 2010 Apr 1;184(7):3417-23
references: 20176738
references: Immun Ageing. 2012 Sep 12;9(1):19
references: 22971545
references: J Exp Med. 2002 Mar 18;195(6):789-94
references: 11901204
references: Nephrol Dial Transplant. 2007 Nov;22(11):3298-303
references: 17597084
references: Nephrol Dial Transplant. 2010 Nov;25(11):3640-6
references: 20400452
references: Kidney Int. 2000 Oct;58(4):1758-64
references: 11012910
references: Cytometry A. 2008 Nov;73(11):975-83
references: 18785267
references: J Exp Med. 2005 Sep 5;202(5):673-85
references: 16147978
references: Annu Rev Med. 2011;62:141-55
references: 21090961
references: J Clin Immunol. 2004 Nov;24(6):612-6
references: 15622445
references: Kidney Int. 2004 Aug;66(2):614-21
references: 15253714
references: Immunology. 2008 Jul;124(3):388-400
references: 18194268
references: J Am Soc Nephrol. 2008 Aug;19(8):1483-90
references: 18480314
references: Nat Protoc. 2006;1(5):2365-76
references: 17406480
references: Trends Immunol. 2002 Dec;23(12):580-5
references: 12464569
references: AIDS. 2011 Sep 24;25(15):1813-22
references: 21412126
references: Kidney Int. 2008 Sep;74(6):760-7
references: 18615000
references: Exp Gerontol. 2002 Jan-Mar;37(2-3):445-53
references: 11772532
references: Nat Immunol. 2011 Jun;12(6):492-9
references: 21739672
references: J Antimicrob Chemother. 2006 Apr;57(4):585-8
references: 16504998
references: PLoS One. 2010 Nov 17;5(11):e14013
references: 21103338
references: Clin Immunol. 1999 Feb;90(2):213-9
references: 10080833
references: J Immunol. 2003 Apr 15;170(8):4342-8
references: 12682271
references: Clin Exp Immunol. 2009 Mar;155(3):423-32
references: 19220832
references: Immun Ageing. 2011 Jan 08;8(1):2
references: 21214947
references: Blood Purif. 2002;20(5):477-80
references: 12207097
references: Nat Rev Nephrol. 2013 May;9(5):255-65
references: 23507826
references: Curr Biol. 2010 Nov 23;20(22):R970-1
references: 21093786
references: Lancet. 1999 Jul 10;354(9173):93-9
references: 10408483
references: J Nephrol. 2012 Mar-Apr;25(2):183-91
references: 22135034
references: Circulation. 2009 Oct 6;120(14):1364-72
references: 19770396
references: Nephrol Dial Transplant. 2009 Oct;24(10):3225-31
references: 19589786