title: Fruit Body Formation on Silkworm by Cordyceps militaris.
abstract: Injection inoculation protocols for fruit body formation of Cordyceps militaris (C. militaris) were investigated to improve the incidence of infection in the silkworm species Bombyx mori (B. mori). Injection, with suspensions of C. militaris hyphal bodies into living silkworm pupae, was used to test for fruit body production. Use of Daeseungjam rather than Baegokjam or Keumokjam varieties of B. mori is thought to be suitable for infection by C. militaris. From mounting, nine-day-old to 11-day-old pupae showed the best incidence of infection with a 100 µL injection volume. Silkworm pupae injected with a hyphal suspension concentration of more than 2 × 10(5) colony-forming unit (cfu) recorded a greater than 96% incidence of infection. Also, fruit bodies of C. militaris were induced and produced at a light intensity between 500 and 1,000 lx.
authors: National Academy of Agricultural Science, Rural Development Administration, Suwon 441-707, Korea.
authors: In-Pyo
authors: IP
authors: Hong
authors: Pil-Don
authors: PD
authors: Kang
authors: Ki-Young
authors: KY
authors: Kim
authors: Sung-Hee
authors: SH
authors: Nam
authors: Man-Young
authors: MY
authors: Lee
authors: Yong-Soo
authors: YS
authors: Choi
authors: Nam-Suk
authors: NS
authors: Kim
authors: Hye-Kyung
authors: HK
authors: Kim
authors: Kwang-Gill
authors: KG
authors: Lee
authors: Richard A
authors: RA
authors: Humber
keywords: Bombyx mori; Cordyceps militaris; Fruit body; Hyphal bodies; Injection inoculation
references: Biochim Biophys Acta. 1965 Aug 10;103(4):646-53
references: 5861260