title: Chemical constituents antioxidant and anticholinesterasic activity of Tabernaemontana catharinensis.
abstract: The present work aimed to analyze the alkaloid content of the ethanolic extract of Tabernaemontana catharinensis (Apocynaceae family) and its fractions as well as to evaluate their antioxidant and anticholinesterasic activities. The analyses of the ethanolic extract of T. catharinensis by mass spectrometry allowed identifying the presence of the alkaloids 16-epi-affinine, coronaridine-hydroxyindolenine, voachalotine, voacristine-hydroxyindolenine, and 12-methoxy-n-methyl-voachalotine, as well as an alkaloid with m/z 385.21 whose spectrum suggests a derivative of voacristine or voacangine. The extract and its alkaloid rich fractions showed antioxidant activity, especially those that contain the alkaloid m/z 385.21 or 16-epi-affinine with DPPH scavenging activity (IC50) between 37.18 and 74.69 μg/mL. Moreover, the extract and its fractions exhibited anticholinesterasic activity, particularly the fractions characterized by the presence of 12-methoxy-n-methyl-voachalotine, with IC50 = 2.1 to 2.5 μg/mL. Fractions with 16-epi-affinine combined good antioxidant (IC50 = 65.59 to 74.69 μg/mL) and anticholinesterasic (IC50 = 7.7 to 8.3 μg/mL) activities, representing an option for further studies aimed at treating neurodegenerative diseases.
authors: Instituto de Biotecnologia, Universidade de Caxias do Sul, Caxias do Sul, RS, Brazil. carla1982n@hotmail.com
authors: Carla
authors: C
authors: Nicola
authors: Mirian
authors: M
authors: Salvador
authors: Adriana Escalona
authors: AE
authors: Gower
authors: Sidnei
authors: S
authors: Moura
authors: Sergio
authors: S
authors: Echeverrigaray
mesh_terms: D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D002800:Cholinesterase Inhibitors / Q000737:chemistry / Q000494:pharmacology*; D002855:Chromatography, Thin Layer; D020128:Inhibitory Concentration 50; D007700:Kinetics; D021241:Spectrometry, Mass, Electrospray Ionization; D030014:Tabernaemontana / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D002800:Cholinesterase Inhibitors
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