title: PICH: a DNA translocase specially adapted for processing anaphase bridge DNA.
abstract: The Plk1-interacting checkpoint helicase (PICH) protein localizes to ultrafine anaphase bridges (UFBs) in mitosis alongside a complex of DNA repair proteins, including the Bloom's syndrome protein (BLM). However, very little is known about the function of PICH or how it is recruited to UFBs. Using a combination of microfluidics, fluorescence microscopy, and optical tweezers, we have defined the properties of PICH in an in vitro model of an anaphase bridge. We show that PICH binds with a remarkably high affinity to duplex DNA, resulting in ATP-dependent protein translocation and extension of the DNA. Most strikingly, the affinity of PICH for binding DNA increases with tension-induced DNA stretching, which mimics the effect of the mitotic spindle on a UFB. PICH binding also appears to diminish force-induced DNA melting. We propose a model in which PICH recognizes and stabilizes DNA under tension during anaphase, thereby facilitating the resolution of entangled sister chromatids.
authors: LaserLaB Amsterdam and Department of Physics, VU University Amsterdam, De Boelelaan 1081, 1081HV Amsterdam, The Netherlands.
authors: Andreas
authors: A
authors: Biebricher
authors: Weatherall Institute of Molecular Medicine, University of Oxford, John Radcliffe Hospital, Oxford, OX3 9DS, U. K.
authors: Seiki
authors: S
authors: Hirano
authors: Nordea Center for Healthy Aging, Department of Cellular and Molecular Medicine, Panum Institute 18.1, University of Copenhagen, Blegdamsvej 3B, 2200 Copenhagen N, Denmark.
authors: Jacqueline H
authors: JH
authors: Enzlin
authors: Centre for Gene Regulation and Expression, College of Life Sciences, University of Dundee, Dundee, DD1 5EH, U.K.
authors: Nicola
authors: N
authors: Wiechens
authors: Novo Nordisk Foundation Center for Protein Research, Panum Institute, University of Copenhagen, Blegdamsvej 3B, 2200 Copenhagen N, Denmark.
authors: Werner W
authors: WW
authors: Streicher
authors: Nordea Center for Healthy Aging, Department of Cellular and Molecular Medicine, Panum Institute 18.1, University of Copenhagen, Blegdamsvej 3B, 2200 Copenhagen N, Denmark.|Novo Nordisk Foundation Center for Protein Research, Panum Institute, University of Copenhagen, Blegdamsvej 3B, 2200 Copenhagen N, Denmark.
authors: Diana
authors: D
authors: Huttner
authors: Biozentrum, University of Basel, Klingelbergstrasse 50/70, CH-4056, Switzerland.
authors: Lily H-C
authors: LH
authors: Wang
authors: Biozentrum, University of Basel, Klingelbergstrasse 50/70, CH-4056, Switzerland.
authors: Erich A
authors: EA
authors: Nigg
authors: Centre for Gene Regulation and Expression, College of Life Sciences, University of Dundee, Dundee, DD1 5EH, U.K.
authors: Tom
authors: T
authors: Owen-Hughes
authors: Nordea Center for Healthy Aging, Department of Cellular and Molecular Medicine, Panum Institute 18.1, University of Copenhagen, Blegdamsvej 3B, 2200 Copenhagen N, Denmark.
authors: Ying
authors: Y
authors: Liu
authors: LaserLaB Amsterdam and Department of Physics, VU University Amsterdam, De Boelelaan 1081, 1081HV Amsterdam, The Netherlands.
authors: Erwin
authors: E
authors: Peterman
authors: LaserLaB Amsterdam and Department of Physics, VU University Amsterdam, De Boelelaan 1081, 1081HV Amsterdam, The Netherlands.
authors: Gijs J L
authors: GJL
authors: Wuite
authors: Weatherall Institute of Molecular Medicine, University of Oxford, John Radcliffe Hospital, Oxford, OX3 9DS, U. K.|Nordea Center for Healthy Aging, Department of Cellular and Molecular Medicine, Panum Institute 18.1, University of Copenhagen, Blegdamsvej 3B, 2200 Copenhagen N, Denmark.
authors: Ian D
authors: ID
authors: Hickson
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000705:Anaphase / Q000235:genetics*; D000818:Animals; D002842:Chromatids / Q000378:metabolism; D004265:DNA Helicases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008856:Microscopy, Fluorescence / Q000379:methods; D009692:Nucleic Acid Heteroduplexes / Q000378:metabolism; D009707:Nucleosomes / Q000378:metabolism; D021381:Protein Transport; D011994:Recombinant Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D009692:Nucleic Acid Heteroduplexes; D009707:Nucleosomes; D011994:Recombinant Proteins; D000255:Adenosine Triphosphate; D004265:DNA Helicases; C516980:ERCC6L protein, human
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