title: Identification of Salmonella Typhimurium-specific DNA aptamers developed using whole-cell SELEX and FACS analysis.
abstract: Conventional methods for detection of infective organisms, such as Salmonella, are complicated and require multiple steps, and the need for rapid detection has increased. Biosensors show great potential for rapid detection of pathogens. In turn, aptamers have great potential for biosensor assay development, given their small size, ease of synthesis and labeling, lack of immunogenicity, a lower cost of production than antibodies, and high target specificity. In this study, ssDNA aptamers specific to Salmonella Typhimurium were obtained by a whole bacterium-based systematic evolution of ligands by exponential enrichment (SELEX) procedure and applied to probing S. Typhimurium. After 10 rounds of selection with S. Typhimurium as the target and Salmonella Enteritidis, Escherichia coli and Staphylococcus aureus as counter targets, the highly enriched oligonucleic acid pool was sorted using flow cytometry. In total, 12 aptamer candidates from different families were sequenced and grouped. Fluorescent analysis demonstrated that aptamer C4 had particularly high binding affinity and selectivity; this aptamer was then further characterized.
authors: Department of Agricultural Engineering, National Academy of Agricultural Sciences, Suwon 441-707, South Korea.
authors: Jihea
authors: J
authors: Moon
authors: Giyoung
authors: G
authors: Kim
authors: Sangdae
authors: S
authors: Lee
authors: Saetbyeol
authors: S
authors: Park
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry / Q000235:genetics*; D015374:Biosensing Techniques / Q000379:methods; D004926:Escherichia coli / Q000235:genetics / Q000302:isolation & purification; D005434:Flow Cytometry / Q000379:methods; D015723:Gene Library; D008024:Ligands; D052156:SELEX Aptamer Technique / Q000379:methods*; D012477:Salmonella enteritidis / Q000235:genetics / Q000302:isolation & purification; D012486:Salmonella typhimurium / Q000235:genetics / Q000302:isolation & purification*; D012680:Sensitivity and Specificity; D013211:Staphylococcus aureus / Q000235:genetics / Q000302:isolation & purification
chemical_list: D052157:Aptamers, Nucleotide; D008024:Ligands
keywords: Aptamer; Bacteria-base SELEX; FACS analysis; Salmonella Typhimurium; ssDNA