title: A workflow from untargeted LC-MS profiling to targeted natural product isolation.
abstract: Liquid chromatography-mass spectrometry (LC-MS or HPLC-MS) is an extremely sensitive analytical technique that enables the detection of metabolites with a vast range of chemistries and molecular masses. Extracts from any biological starting material are first fractionated chromatographically on a stationary phase suitable for the retention of the target molecules. The eluent is then transferred directly to the ionization source for MS detection. There is a vast range of chromatographic separation methods and MS configurations. This chapter describes a method for the detection of a broad range of metabolites using reversed phase (C18) LC-MS as well as a method for the isolation of targeted metabolites of interest.
authors: Metabolomics Australia, School of Botany, The University of Melbourne, Parkville, VIC, Australia.
authors: Damien L
authors: DL
authors: Callahan
authors: Candace E
authors: CE
authors: Elliott
mesh_terms: D001688:Biological Products / Q000737:chemistry* / Q000302:isolation & purification*; D002853:Chromatography, Liquid / Q000379:methods*; D013058:Mass Spectrometry / Q000379:methods*
chemical_list: D001688:Biological Products