title: Expression of hyaluronan (hyaluronic acid) in the developing laminar architecture of the human fetal brain.
abstract: Hyaluronan (also called hyaluronic acid or HA) plays a key role in the morphogenesis of the brain, but little is known about its expression in the human fetal neocortex. Using immunohistochemical methods, we assayed the expression of HA, glial fibrillary acidic protein, vimentin, nestin, and proliferating cell nuclear antigen in paraffin-embedded histologic sections of 8 mid-term fetuses (estimated gestational age, 12-16 weeks; crown-rump length, 75-120mm). At 12-13 weeks, HA was expressed strongly along the membranes of many cells in the cortical plate and the layer 1 or marginal zone, but showed weak, spotty expression in a fiber-rich layer adjacent to the cortical plate, called the cortical stratified transitional field-1 (STF-1 or a primitive form of the subplate). At 15-16 weeks, HA was expressed in the layer 1 and in the early subplate or presubplate, but less strongly in cells of the possible STF-5 near the subventricular zone. However, the positive observation in STF-5 was probably a result of individual difference in development. The developing cortical plate seemed to produce HA in the presubplate to harbor axonal plexus of various afferent systems, while Cajal-Retzius cells were likely to accumulate HA in the layer 1. The HA-rich zones, those sandwiched the cortical plate, might avoid further migration of cortical cells.
authors: Maxillofacial Anatomy, Department of Maxillofacial Biology, Tokyo Medical and Dental University Graduate School, Tokyo, Japan.
authors: Shunichi
authors: S
authors: Shibata
authors: Kwang Ho
authors: KH
authors: Cho
authors: Ji Hyun
authors: JH
authors: Kim
authors: Hiroshi
authors: H
authors: Abe
authors: Gen
authors: G
authors: Murakami
authors: Baik Hwan
authors: BH
authors: Cho
mesh_terms: D000328:Adult; D001921:Brain / Q000196:embryology*; D001923:Brain Chemistry; D003337:Corpus Callosum / Q000166:cytology / Q000378:metabolism; D005260:Female; D005865:Gestational Age; D006801:Humans; D006820:Hyaluronic Acid / Q000096:biosynthesis*; D007150:Immunohistochemistry; D007382:Intermediate Filaments / Q000378:metabolism; D019579:Neocortex / Q000196:embryology; D064231:Nestin / Q000378:metabolism; D016612:Paraffin Embedding; D011247:Pregnancy; D016707:Tissue Fixation; D014746:Vimentin / Q000378:metabolism
chemical_list: D064231:Nestin; D014746:Vimentin; D006820:Hyaluronic Acid
keywords: Cajal–Retzius cells; Cortical stratified transitional field; Human fetus; Hyaluronan; Neocortex; Subplate