title: High temperature aqueous ammonia pretreatment and post-washing enhance the high solids enzymatic hydrolysis of corn stover.
abstract: Aqueous ammonia pretreatment was optimized and the limiting factors in high solids enzymatic hydrolysis were assessed. The recommended pretreatment condition to achieve high enzymatic yield was: 180 °C, 20% (w/w) ammonia, 30 min, and 20% solids content. FT-IR and GC-MS results indicated that most of the lignin was degraded to soluble fragments after pretreatment. The pretreated solids after post-washing showed higher enzymatic digestibility at high solids loading than that without washing. The washed solids required lower cellulase and xylanase dosage than unwashed solids to achieve high sugar yield. Enzymatic conversions were declined with the increased solids loading of pretreated solids, pretreated-washed solids, and filter papers. The results indicated that solids loading in enzymatic hydrolysis was an important factor affecting sugar yield. The increasing concentration of glucose and ligno-phenolics mainly inhibited the enzymatic hydrolysis of aqueous ammonia pretreated corn stover.
authors: Key Laboratory of Systems Bioengineering, Ministry of Education, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, PR China.
authors: Lei
authors: L
authors: Qin
authors: Key Laboratory of Systems Bioengineering, Ministry of Education, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, PR China.
authors: Zhi-Hua
authors: ZH
authors: Liu
authors: Biomass Conversion Research Lab, Department of Chemical Engineering and Materials Science, Michigan State University, 3815 Technology Boulevard, Lansing, MI 48910, USA.
authors: Mingjie
authors: M
authors: Jin
authors: Key Laboratory of Systems Bioengineering, Ministry of Education, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, PR China. Electronic address: bzli@tju.edu.cn.
authors: Bing-Zhi
authors: BZ
authors: Li
authors: Key Laboratory of Systems Bioengineering, Ministry of Education, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, PR China.
authors: Ying-Jin
authors: YJ
authors: Yuan
mesh_terms: D000641:Ammonia / Q000737:chemistry*; D056804:Biofuels; D018533:Biomass; D002241:Carbohydrates / Q000737:chemistry*; D002480:Cellulase / Q000737:chemistry; D002482:Cellulose / Q000737:chemistry; D005374:Filtration; D008401:Gas Chromatography-Mass Spectrometry; D005947:Glucose / Q000737:chemistry*; D006868:Hydrolysis; D019800:Phenol / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D013696:Temperature; D013997:Time Factors; D014867:Water / Q000737:chemistry; D014990:Xylans / Q000737:chemistry; D003313:Zea mays
chemical_list: D056804:Biofuels; D002241:Carbohydrates; D014990:Xylans; D014867:Water; D019800:Phenol; D000641:Ammonia; D002482:Cellulose; D002480:Cellulase; D005947:Glucose
keywords: Aqueous ammonia pretreatment; Corn stover; Enzymatic hydrolysis; High solids loading; Post-washing