title: Subdiffusion supports joining of correct ends during repair of DNA double-strand breaks.
abstract: The mobility of damaged chromatin regions in the nucleus may affect the probability of mis-repair. In this work, live-cell observation and distance tracking of GFP-tagged DNA damage response protein MDC1 was used to study the random-walk behaviour of chromatin domains containing radiation-induced DNA double-strand breaks (DSB). Our measurements indicate a subdiffusion-type random walk process with similar time dependence for isolated and clustered DSBs that were induced by 20 MeV proton or 43 MeV carbon ion micro-irradiation. As compared to normal diffusion, subdiffusion enhances the probability that both ends of a DSB meet, thus promoting high efficiency DNA repair. It also limits their probability of long-range movements and thus lowers the probability of mis-rejoining and chromosome aberrations.
authors: Angewandte Physik und Messtechnik LRT2, Universität der Bundeswehr München, 85577 Neubiberg, Germany. stefanie.girst@unibw.de
authors: S
authors: S
authors: Girst
authors: V
authors: V
authors: Hable
authors: G A
authors: GA
authors: Drexler
authors: C
authors: C
authors: Greubel
authors: C
authors: C
authors: Siebenwirth
authors: M
authors: M
authors: Haum
authors: A A
authors: AA
authors: Friedl
authors: G
authors: G
authors: Dollinger
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D018797:Cell Cycle Proteins; D045744:Cell Line, Tumor; D002843:Chromatin / Q000737:chemistry* / Q000235:genetics; D004249:DNA Damage / Q000235:genetics*; D004260:DNA Repair / Q000235:genetics*; D004273:DNA, Neoplasm / Q000737:chemistry* / Q000235:genetics*; D004058:Diffusion; D006801:Humans; D008957:Models, Genetic; D015233:Models, Statistical; D009687:Nuclear Proteins / Q000737:chemistry* / Q000235:genetics*; D012516:Osteosarcoma / Q000737:chemistry / Q000235:genetics; D011485:Protein Binding; D015534:Trans-Activators / Q000737:chemistry* / Q000235:genetics*
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D018797:Cell Cycle Proteins; D002843:Chromatin; D004273:DNA, Neoplasm; C413163:MDC1 protein, human; D009687:Nuclear Proteins; D015534:Trans-Activators
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