title: A novel aptamer-based competition strategy for ultrasensitive electrochemical detection of leukemia cells.
abstract: A robust, nanobiotechnology-based electrochemical cytosensing platform for the detection of acute leukemia cells was developed with high sensitivity, selectivity, acceptable rapidity and excellent extensibility. It utilized the competitive binding of cell-specific aptamers to acute leukemia cells and subsequent voltammetric quantification of the metal signature. Greatly enhanced sensitivity was achieved with dual signal amplification by using Fe3O4 magnetic nanoparticles (MNPs) as carriers to load a large amount of gold nanoparticles (AuNPs) and AuNP-catalyzed silver deposition. The proposed competitive cytosensor showed high sensitivity with a detection limit down to 10 cells. This simple and low-cost electrochemical cytosensing approach offers great promise to extend its application to early detection of human leukemia and possibly to other cancer cells.
authors: The Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing University, Nanjing, 210008, P. R. China.
authors: Kui
authors: K
authors: Zhang
authors: Tingting
authors: T
authors: Tan
authors: Jia-Ju
authors: JJ
authors: Fu
authors: Tingting
authors: T
authors: Zheng
authors: Jun-Jie
authors: JJ
authors: Zhu
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry* / Q000378:metabolism; D015374:Biosensing Techniques / Q000379:methods*; D055664:Electrochemical Techniques / Q000379:methods*; D018922:HL-60 Cells; D006367:HeLa Cells; D006801:Humans; D020014:K562 Cells; D007938:Leukemia / Q000378:metabolism; D053768:Metal Nanoparticles / Q000737:chemistry*; D011485:Protein Binding / Q000502:physiology
chemical_list: D052157:Aptamers, Nucleotide