title: Evidence of dynamically dysregulated gene expression pathways in hyperresponsive B cells from African American lupus patients.
abstract: Recent application of gene expression profiling to the immune system has shown a great potential for characterization of complex regulatory processes. It is becoming increasingly important to characterize functional systems through multigene interactions to provide valuable insights into differences between healthy controls and autoimmune patients. Here we apply an original systematic approach to the analysis of changes in regulatory gene interconnections between in Epstein-Barr virus transformed hyperresponsive B cells from SLE patients and normal control B cells. Both traditional analysis of differential gene expression and analysis of the dynamics of gene expression variations were performed in combination to establish model networks of functional gene expression. This Pathway Dysregulation Analysis identified known transcription factors and transcriptional regulators activated uniquely in stimulated B cells from SLE patients.
authors: University of Texas Southwestern Medical Center, Dallas, Texas, United States of America.
authors: Igor
authors: I
authors: Dozmorov
authors: Nicolas
authors: N
authors: Dominguez
authors: Andrea L
authors: AL
authors: Sestak
authors: Julie M
authors: JM
authors: Robertson
authors: John B
authors: JB
authors: Harley
authors: Judith A
authors: JA
authors: James
authors: Joel M
authors: JM
authors: Guthridge
mesh_terms: D001402:B-Lymphocytes / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D044383:Black People; D016022:Case-Control Studies; D016207:Cytokines / Q000235:genetics / Q000378:metabolism; D005260:Female; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D053263:Gene Regulatory Networks; D006801:Humans; D007140:Immunoglobulin Fab Fragments / Q000494:pharmacology; D008180:Lupus Erythematosus, Systemic / Q000208:ethnology / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D019950:Mitogen-Activated Protein Kinase 1 / Q000235:genetics / Q000378:metabolism; D048052:Mitogen-Activated Protein Kinase 3 / Q000235:genetics / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D061251:Primary Cell Culture; D025941:Protein Interaction Mapping; D015398:Signal Transduction; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D001741:Black or African American
chemical_list: D016207:Cytokines; D007140:Immunoglobulin Fab Fragments; D014157:Transcription Factors; C535150:MAPK1 protein, human; D019950:Mitogen-Activated Protein Kinase 1; D048052:Mitogen-Activated Protein Kinase 3
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