title: The physiology of ex vitro pineapple (Ananas comosus L. Merr.  var MD-2) as CAM or C3 is regulated by the environmental  conditions: proteomic and transcriptomic profiles.
abstract: CONCLUSIONS
  Proteomic and transcriptomic profiles of key enzymes were monitored in pineapple plants propagated under C3 and CAM-inducing metabolisms to obtain insight into the CAM-facultative metabolism and the relationship of CAM plants with oxidative stress.
  Pineapple is one of the most important tropical crops worldwide. The use of temporary immersion bioreactors for the first stages of pineapple propagation enables precise control of plant growth, increases the rate of plant multiplication, decreases space, energy and labor requirements for pineapple plants in commercial micropropagation. Once the plantlets are ready to be taken from the reactors, they are carefully acclimatized to natural environmental conditions, and a facultative C3/CAM metabolism in the first 2 months of growth is the characteristic of pineapple plants, depending on environmental conditions. We subjected two sets of micropropagated pineapple plants to C3 and CAM-inducing environmental conditions, determined by light intensity/relative humidity (respectively 40 μmol m−2 s−1/85 % and 260 μmol m−2 s−1/50 %). Leaves of pineapple plants grown under CAM-inducing conditions showed higher leaf thickness and more developed cuticles and hypodermic tissue. Proteomic profiles of several proteins, isoenzyme patterns and transcriptomic profiles were also measured. Five major spots were isolated and identified, two of them for the first time in Ananas comosus (OEE 1; OEE 2) and the other three corresponding to small fragments of the large subunit of Rubisco (LSU). PEPC and PEPCK were also detected by immunobloting of 2DE at the end of both ex vitro treatments (C3/CAM) during the dark period. Isoenzymes of SOD and CAT were identified by electrophoresis and the transcript levels of OEE 1 and CAT were associated with CAM metabolism in pineapple plants.
authors: C
authors: C
authors: Aragón
authors: P
authors: P
authors: Pascual
authors: J
authors: J
authors: González
authors: M
authors: M
authors: Escalona
authors: L
authors: L
authors: Carvalho
authors: S
authors: S
authors: Amancio
mesh_terms: D000595:Amino Acid Sequence; D031230:Ananas / Q000033:anatomy & histology / Q000201:enzymology / Q000235:genetics* / Q000502:physiology*; D002244:Carbon / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D004777:Environment; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D006863:Hydrogen-Ion Concentration; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D053147:Plant Exudates / Q000378:metabolism; D018515:Plant Leaves / Q000033:anatomy & histology / Q000201:enzymology / Q000235:genetics; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D040901:Proteomics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
chemical_list: D053147:Plant Exudates; D010940:Plant Proteins; D012333:RNA, Messenger; D002244:Carbon
references: FEBS Lett. 2002 Jul 17;523(1-3):138-42
references: 12123820
references: Biochem J. 1996 Aug 15;318 ( Pt 1):227-34
references: 8761476
references: Nature. 1970 Aug 15;227(5259):680-5
references: 5432063
references: Anal Biochem. 1976 May 7;72:248-54
references: 942051
references: BMC Res Notes. 2012 May 07;5:220
references: 22564373
references: Plant Physiol. 1997 Jan;113(1):249-257
references: 12223604
references: Plant Cell Rep. 2012 Apr;31(4):757-69
references: 22134875
references: Int J Biol Sci. 2007 Dec 07;4(1):8-14
references: 18167531
references: Photosynth Res. 2003;77(2-3):241-54
references: 16228379
references: J Exp Bot. 2011 May;62(9):3109-18
references: 21430293
references: Plant J. 1997 Apr;11(4):871-82
references: 9161042
references: Plant Cell Physiol. 2001 Feb;42(2):236-9
references: 11230579
references: Proc Natl Acad Sci U S A. 2004 Mar 9;101(10):3703-8
references: 14982989
references: Ann Bot. 2004 Jun;93(6):629-52
references: 15150072
references: Annu Rev Plant Physiol Plant Mol Biol. 1996 Jun;47:273-298
references: 15012290
references: J Exp Bot. 2008;59(7):1895-908
references: 18408220
references: Plant Physiol. 1996 Jan;110(1):125-36
references: 8587977
references: J Exp Bot. 2004 May;55(400):1255-65
references: 15073222
references: Plant Physiol. 1995 Dec;109(4):1247-1257
references: 12228666
references: Trends Plant Sci. 2000 Feb;5(2):75-80
references: 10664617
references: J Biol Chem. 2005 Apr 22;280(16):16170-4
references: 15722336