title: A simplified two-stitch sleeve technique for arterial anastomosis of cervical heterotopic cardiac transplantation in mice.
abstract: Although cervical cardiac transplantation is a well recognized useful model in diverse experimental settings, its widespread use, however, has been significantly hampered by the technical challenges relevant to small vessel anastomosis. We herein introduced a simplified two-stitch sleeve technique into arterial anastomosis during the course of cervical cardiac transplantation in mice. Cervical transplantation of allogenic and syngeneic cardiac grafts was conducted to assess the feasibility of this two-stitch sleeve technique in arterial anastomosis. Venous anastomosis was completed by the one-suture end-to-end microsuture technique, while arterial anastomosis was conducted by invaginating the recipient right common carotid artery into the graft left common carotid artery along with two guiding stitches. The two-stitch sleeve technique significantly simplified the procedures for arterial anastomosis as compared with that of the traditional microsuture technique (5.5 ± 1.8 min vs. 15.7 ± 3.0 min). However, the survival time for allografts (8.0 ± 0.2 day vs. 8.0 ± 0.4 day) and the long-term patency for syngeneic grafts (> 120 days) were the same as the grafts implanted by the traditional microsuture technique. This simplified sleeve technique is easy to learn, particularly for beginners without microsuture experience, and therefore, it has the great potential for widespread use in transplant immunology.
authors: The Center for Biomedical Research, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology Wuhan 430030, China ; Key Laboratory of Organ Transplantation, Ministry of Education, Ministry of Health, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology Wuhan 430030, China ; Department of Cardiovascular Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology Wuhan 430030, China.
authors: Jing
authors: J
authors: Fang
authors: Long
authors: L
authors: He
authors: Shi-Qiang
authors: SQ
authors: Wang
authors: Ming-Jia
authors: MJ
authors: Ma
authors: Hong-Yun
authors: HY
authors: Liu
authors: Xue-Hai
authors: XH
authors: Zhu
authors: Ping
authors: P
authors: Zhu
authors: Xiang
authors: X
authors: Wei
authors: Cong-Yi
authors: CY
authors: Wang
keywords: Anastomosis; heart transplantation; microsuture; two-stitch sleeve technique
references: J Hand Surg Am. 1990 Jul;15(4):619-25
references: 2380526
references: Plast Reconstr Surg. 1988 May;81(5):725-34
references: 3362988
references: Transplantation. 1991 Dec;52(6):1099-101
references: 1750075
references: Microsurgery. 1995;16(12):793-802
references: 8844661
references: Circulation. 2011 Jun 28;123(25):2964-74
references: 21670230
references: J Vis Exp. 2007;(6):238
references: 18997886
references: Transplantation. 1991 Sep;52(3):562-4
references: 1897034
references: J Heart Lung Transplant. 1999 Oct;18(10):946-51
references: 10561104
references: Microsurgery. 2008;28(7):565-70
references: 18767132
references: Scand J Plast Reconstr Surg Hand Surg. 2004;38(1):1-4
references: 15074715
references: Scand J Plast Reconstr Surg. 1978;12(3):291-4
references: 368973
references: Am J Pathol. 2012 Sep;181(3):937-46
references: 22796409
references: Am J Transplant. 2007 Apr;7(4):799-808
references: 17331117
references: J Heart Lung Transplant. 2009 Oct;28(10):1102-6
references: 19782294
references: Transplant Proc. 1973 Mar;5(1):733-5
references: 4572133
references: Plast Reconstr Surg. 1988 Dec;82(6):1102-3
references: 3059385
references: J Surg Res. 1989 Mar;46(3):189-94
references: 2646472
references: Plast Reconstr Surg. 1982 Aug;70(2):186-92
references: 7048368
references: Microsurgery. 1996;17(8):472-6
references: 9393668
references: Br J Plast Surg. 1987 May;40(3):288-94
references: 3594059
references: J Heart Lung Transplant. 2001 Oct;20(10):1123-8
references: 11595568
references: Microsurgery. 1996;17(6):291-4
references: 9308711