title: Effect of sex sorting on stallion spermatozoa: Heterologous oocyte binding, tyrosine phosphorylation and acrosome reaction assay.
abstract: The interest on sex sorting by flow cytometry on the equine industry has been increasing over the years. In this work, three different tests were performed in order to evaluate the membrane status of sorted stallion spermatozoa: assessment of binding ability to porcine oocytes, evaluation of acrosome integrity after stimulation with A23187, and detection of tyrosine phosphorylation. These evaluations were made after incubation for 0h, 1.5h and 3h in a capacitating medium. Sorted stallion spermatozoa attached similarly to the porcine oocytes, when compared with control samples. Sorted spermatozoa were more prone to undergo acrosome reaction (P<0.05), at the beginning and after 1.5h and 3h of incubation, and also had higher tyrosine phosphorylation of the tail (P<0.001), only at the beginning of the incubation period. Apparently sex sorted stallion spermatozoa are in a more advanced status of membrane destabilization, which could be associated with capacitation, although similar binding ability to porcine oocytes is maintained.
authors: Laboratory of Equine Reproduction and Equine Spermatology, Veterinary Teaching Hospital, University of Extremadura, Cáceres, Spain. carolinabalaosilva@gmail.com
authors: C M
authors: CM
authors: Balao da Silva
authors: M
authors: M
authors: Spinaci
authors: D
authors: D
authors: Bucci
authors: E
authors: E
authors: Giaretta
authors: F J
authors: FJ
authors: Peña
authors: G
authors: G
authors: Mari
authors: G
authors: G
authors: Galeati
mesh_terms: D020101:Acrosome Reaction / Q000502:physiology*; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D000001:Calcimycin / Q000494:pharmacology; D002470:Cell Survival; D006736:Horses / Q000502:physiology*; D008297:Male; D010766:Phosphorylation / Q000502:physiology; D012743:Sex Preselection / Q000662:veterinary*; D013084:Sperm-Ovum Interactions / Q000502:physiology*; D013094:Spermatozoa / Q000187:drug effects / Q000502:physiology*; D014443:Tyrosine / Q000378:metabolism*
chemical_list: D000900:Anti-Bacterial Agents; D000001:Calcimycin; D014443:Tyrosine
keywords: Binding; Capacitation; Equine; Hoechst 33342; Sex sorting; Spermatozoa