title: Soybean GmbZIP123 gene enhances lipid content in the seeds of transgenic Arabidopsis plants.
abstract: Soybean is one of most important oil crops and a significant increase in lipid content in soybean seeds would facilitate vegetable oil production in the world. Although the pathways for lipid biosynthesis in higher plants have been uncovered, our understanding of regulatory mechanism controlling lipid accumulation is still limited. In this study, we identified 87 transcription factor genes with a higher abundance at the stage of lipid accumulation in soybean seeds. One of these genes, GmbZIP123, was selected to further study its function in regulation of lipid accumulation. Overexpression of GmbZIP123 enhanced lipid content in the seeds of transgenic Arabidopsis thaliana plants. The GmbZIP123 transgene promoted expression of two sucrose transporter genes (SUC1 and SUC5) and three cell-wall invertase genes (cwINV1, cwINV3, and cwINV6) by binding directly to the promoters of these genes. Consistently, the cell-wall invertase activity and sugar translocation were all enhanced in siliques of GmbZIP123 transgenic plants. Higher levels of glucose, fructose, and sucrose were also found in seeds of GmbZIP123 transgenic plants. These results suggest that GmbZIP123 may participate in regulation of lipid accumulation in soybean seeds by controlling sugar transport into seeds from photoautotrophic tissues. This study provides novel insights into the regulatory mechanism for lipid accumulation in seeds and may facilitate improvements in oil production in soybean and other oil crops through genetic manipulation of the GmbZIP123 gene.
authors: State Key Lab of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, PR China.
authors: Qing-Xin
authors: QX
authors: Song
authors: Qing-Tian
authors: QT
authors: Li
authors: Yun-Feng
authors: YF
authors: Liu
authors: Feng-Xia
authors: FX
authors: Zhang
authors: Biao
authors: B
authors: Ma
authors: Wan-Ke
authors: WK
authors: Zhang
authors: Wei-Qun
authors: WQ
authors: Man
authors: Wei-Guang
authors: WG
authors: Du
authors: Guo-Dong
authors: GD
authors: Wang
authors: Shou-Yi
authors: SY
authors: Chen
authors: Jin-Song
authors: JS
authors: Zhang
mesh_terms: D017360:Arabidopsis / Q000235:genetics*; D050260:Carbohydrate Metabolism / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant / Q000235:genetics*; D056726:Genetic Association Studies; D050356:Lipid Metabolism / Q000235:genetics*; D008055:Lipids / Q000096:biosynthesis; D010938:Plant Oils / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic / Q000235:genetics; D011485:Protein Binding / Q000235:genetics; D055503:Protein Multimerization; D021381:Protein Transport; D012639:Seeds / Q000235:genetics*; D013025:Glycine max / Q000235:genetics*; D013347:Subcellular Fractions / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism; D015533:Transcriptional Activation / Q000235:genetics
chemical_list: D008055:Lipids; D010938:Plant Oils; D010940:Plant Proteins; D014157:Transcription Factors
keywords: Cell-wall intertase; GmbZIP123 overexpression; seed lipid; soybean; sucrose transporter.; sugar transport
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