title: ChiS histidine kinase negatively regulates the production of chitinase ChiC in Streptomyces peucetius.
abstract: Computational analysis of sequence homology of the chiSRC gene cluster, encoding a chitinase in Streptomyces peucetius, showed that the gene cluster could be a two-component regulon comprising a sensor kinase (chiS) and a response regulator (chiR). To prove that the ChiSRC is an authentic two-component system, the chiS gene was cloned and expressed in E.coli and the purified protein was used for biochemical analysis. In this report, we provide biochemical evidence to show that the sensor kinase encoded by chiS gene indeed is a histidine kinase capable of autophosphorylation and the histidine 144 residue of the ChiS protein is the phosphate acceptor. An insertion mutation at the chiS locus led to overproduction chitinase protein in S. peucetius implying that the chiC gene is negatively regulated by the two-component system.
authors: Department of Genetic Engineering, School of Biotechnology, Madurai Kamaraj University, Madurai, 625 021, India.
authors: Amrathlal
authors: A
authors: Rabbind Singh
authors: Department of Genetic Engineering, School of Biotechnology, Madurai Kamaraj University, Madurai, 625 021, India; Department of Molecular Genetics, Biochemistry and Microbiology, University of Cincinnati College of Medicine, Cincinnati, OH 45267 - 0524, USA.
authors: Paranthaman
authors: P
authors: Senthamaraikannan
authors: Department of Genetic Engineering, School of Biotechnology, Madurai Kamaraj University, Madurai, 625 021, India.
authors: Chitra
authors: C
authors: Thangavel
authors: Department of Genetic Engineering, School of Biotechnology, Madurai Kamaraj University, Madurai, 625 021, India.
authors: Ravikanth
authors: R
authors: Danda
authors: Department of Genetic Engineering, School of Biotechnology, Madurai Kamaraj University, Madurai, 625 021, India; Department of Biotechnology, Alagappa University, Karaikudi, 630 003, Tamil Nadu, India.
authors: Shunmugiah Karutha
authors: SK
authors: Pandian
authors: Department of Genetic Engineering, School of Biotechnology, Madurai Kamaraj University, Madurai, 625 021, India. Electronic address: dharmalingam.k@gmail.com.
authors: Kuppamuthu
authors: K
authors: Dharmalingam
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D002688:Chitinases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D015971:Gene Expression Regulation, Enzymologic; D000071677:Histidine Kinase; D008969:Molecular Sequence Data; D011494:Protein Kinases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D016415:Sequence Alignment; D013302:Streptomyces / Q000737:chemistry / Q000201:enzymology* / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D011494:Protein Kinases; D000071677:Histidine Kinase; D002688:Chitinases
keywords: Chitinase; E. coli Rosetta2; Histidine kinase; Streptomyces peucetius; Two-component system