title: The tetraspanin CD9 affords high-purity capture of all murine hematopoietic stem cells.
abstract: Prospective isolation is critical for understanding the cellular and molecular aspects of stem cell heterogeneity. Here, we identify the cell surface antigen CD9 as a positive marker that provides a simple alternative for hematopoietic stem cell isolation at high purity. Crucially, CD9 affords the capture of all hematopoietic stem cells in murine bone marrow in the absence of contaminating populations that lack authentic stem cell function. Using CD9 as a tool to subdivide hematopoietic stem-cell-containing populations, we provide evidence for heterogeneity at the cellular, functional, and molecular levels.
authors: Stem Cell Laboratory, University College London Cancer Institute, London WC1E 6BT, UK.
authors: Göran
authors: G
authors: Karlsson
authors: Emma
authors: E
authors: Rörby
authors: Cristina
authors: C
authors: Pina
authors: Shamit
authors: S
authors: Soneji
authors: Kristian
authors: K
authors: Reckzeh
authors: Kenichi
authors: K
authors: Miharada
authors: Christine
authors: C
authors: Karlsson
authors: Yanping
authors: Y
authors: Guo
authors: Cristina
authors: C
authors: Fugazza
authors: Rajeev
authors: R
authors: Gupta
authors: Joost H A
authors: JH
authors: Martens
authors: Hendrik G
authors: HG
authors: Stunnenberg
authors: Stefan
authors: S
authors: Karlsson
authors: Tariq
authors: T
authors: Enver
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000032:analysis; D002469:Cell Separation / Q000379:methods*; D005434:Flow Cytometry / Q000379:methods*; D018380:Hematopoietic Stem Cell Transplantation; D006412:Hematopoietic Stem Cells / Q000737:chemistry / Q000145:classification / Q000166:cytology*; D051379:Mice; D008810:Mice, Inbred C57BL; D060245:Tetraspanin 29 / Q000032:analysis*
chemical_list: D015415:Biomarkers; D060245:Tetraspanin 29