title: Gold-nanoparticles-modified cellulose membrane coupled with laser desorption/ionization mass spectrometry for detection of iodide in urine.
abstract: We report an efficient method for the determination of iodide (I(-)) ions by using gold-iodide hybrid cluster ions on gold nanoparticles (Au NPs) modified mixed cellulose ester membrane (Au NPs-MCEM) by pulsed laser desorption/ionization mass spectrometry (LDI-MS). When I(-) ions were deposited and concentrated on the surfaces of Au NPs (32 nm) via strong Au(+)-I(-) interaction on the MECM, the Au NPs-MCEM was observed to function as an efficient surface-assisted LDI substrate with very low background noise. When pulsed laser radiation (355 nm) was applied, I(-) binding to Au NPs ions induced the enhancement of the desorption and ionization efficiency of gold-iodide hybrid cluster ions from the Au NPs surfaces. The reproducibility of the probe for both shot-to-shot and sample-to-sample (both less than 10%) ion production was also improved by the homogeneous nature of the substrate surface. Thus, it allows the accurate and precise quantification of I(-) ions in high-salinity real samples (i.e., edible salt samples and urine) at the nanomolar range. This novel LDI-MS approach provides a simple route for the high-speed analysis of I(-) ions with high sensitivity and selectivity in real biological samples.
authors: Institute of Bioscience and Biotechnology and ‡Center of Excellence for the Oceans, National Taiwan Ocean University , Keelung, 20224, Taiwan.
authors: Yu-Jia
authors: YJ
authors: Li
authors: Yu-Ting
authors: YT
authors: Tseng
authors: Binesh
authors: B
authors: Unnikrishnan
authors: Chih-Ching
authors: CC
authors: Huang
mesh_terms: D001205:Ascorbic Acid / Q000737:chemistry; D002482:Cellulose / Q000031:analogs & derivatives* / Q000737:chemistry; D006046:Gold / Q000737:chemistry*; D007454:Iodides / Q000652:urine*; D053768:Metal Nanoparticles / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D007454:Iodides; C020253:cellulose ester plastic; D006046:Gold; D002482:Cellulose; D001205:Ascorbic Acid