title: Seeding experiments demonstrate poor performance of the hatching test for detecting small numbers of Schistosoma mansoni eggs in feces.
abstract: Parasitological methods for the evaluation of schistosomiasis tend to be limited when parasitic burdens are low, which is a major characteristic of low intensity transmission areas. While the hatching test (HT) method has been considered to be "very sensitive", reports of its capacity to detect low numbers of eggs remain scarce in the published literature. Our main hypothesis is that HT has limitations and cannot be recommended for diagnosing light infections or as a control of cure. Hence, this study aims to describe the performance of HT in detail, with respect to seeding experiments for egg numbers in the range of 4 to 24 eggs per gram (epg) of feces. Different numbers of eggs of Schistosoma mansoni were seeded in normal human feces. The first set of experiments evaluated the amount of feces (higher than 0.5 g prevented hatching), the proximity of the light source (50 cm was preferred), and the observation time required for the detection of miracidia (more than 3h did not add to sensitivity). HT was subsequently performed with 12, 10, 8, 6, 4, and 2 eggs in 0.5 g of feces. The final set of experiments was performed to analyze the initial filtration step, in which surgical gauze versus a 500 μm nylon mesh was compared and demonstrated losses of eggs that occurred with washing and gauze (better with nylon) sieving steps. The proposed method was found to produce 100% positivity for up to 12 epg, with a sharp decrease to 33% for 8 epg and less. In conclusion, HT is not recommended for diagnosing intestinal schistosomiasis in populations with light infections, considering the complexity of the procedure and its lack of effectiveness with fecal amounts higher than 0.5 g even at optimized conditions.
authors: Grupo de Parasitologia Biomédica da Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS), Avenida Ipiranga 6681, Prédio 12C, 90619-900 Porto Alegre, Brazil.
authors: Dieli Souza
authors: DS
authors: Borges
authors: Juliana Schilling
authors: JS
authors: de Souza
authors: Juliano
authors: J
authors: Romanzini
authors: Carlos
authors: C
authors: Graeff-Teixeira
mesh_terms: D000818:Animals; D005243:Feces / Q000469:parasitology; D005260:Female; D006801:Humans; D051379:Mice; D010063:Ovum; D010270:Parasite Egg Count / Q000379:methods*; D012550:Schistosoma mansoni / Q000502:physiology*; D012555:Schistosomiasis mansoni / Q000175:diagnosis* / Q000469:parasitology
keywords: Coproparasitology; Hatching test; Low endemicity; Miracidia hatching; Schistosomiasis