title: Recombinant antibodies to histone post-translational modifications.
abstract: Variability in the quality of antibodies to histone post-translational modifications (PTMs) is a widely recognized hindrance in epigenetics research. Here, we produced recombinant antibodies to the trimethylated lysine residues of histone H3 with high specificity and affinity and no lot-to-lot variation. These recombinant antibodies performed well in common epigenetics applications, and enabled us to identify positive and negative correlations among histone PTMs.
authors: Department of Biochemistry and Molecular Biology, The University of Chicago, Chicago, Illinois, USA.
authors: Takamitsu
authors: T
authors: Hattori
authors: Joseph M
authors: JM
authors: Taft
authors: Kalina M
authors: KM
authors: Swist
authors: Hao
authors: H
authors: Luo
authors: Heather
authors: H
authors: Witt
authors: Matthew
authors: M
authors: Slattery
authors: Akiko
authors: A
authors: Koide
authors: Alexander J
authors: AJ
authors: Ruthenburg
authors: Krzysztof
authors: K
authors: Krajewski
authors: Brian D
authors: BD
authors: Strahl
authors: Kevin P
authors: KP
authors: White
authors: Peggy J
authors: PJ
authors: Farnham
authors: Yingming
authors: Y
authors: Zhao
authors: Shohei
authors: S
authors: Koide
mesh_terms: D000818:Animals; D000906:Antibodies / Q000235:genetics / Q000276:immunology*; D000915:Antibody Affinity; D001666:Binding Sites, Antibody; D002460:Cell Line; D004926:Escherichia coli / Q000235:genetics; D006657:Histones / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D006801:Humans; D008239:Lysine / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D019151:Peptide Library; D011499:Protein Processing, Post-Translational; D012680:Sensitivity and Specificity; D057127:Single-Chain Antibodies / Q000235:genetics / Q000276:immunology
chemical_list: D000906:Antibodies; D006657:Histones; D019151:Peptide Library; D057127:Single-Chain Antibodies; D008239:Lysine
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