title: Different Effects of Six Antibiotics and Ten Traditional Chinese Medicines on Shiga Toxin Expression by Escherichia coli O157:H7.
abstract: This study compared the effects of ten types of traditional Chinese medicines (TCMs) and six different antibiotics on E. coli O157:H7 Shiga toxin gene (stx2) mRNA expression level based on real-time PCR and the expression level of Stx toxin using an ELISA quantitative assay. We also compared their effects on the induction of the SOS response. The results clearly indicated that all ten TCMs had negative results in the SOS response induction test, while most TCMs did not increase the levels of stx2 mRNA and the Stx toxin. Some TCMs did increase the mRNA levels of the stx2 gene and the Stx toxin level, but their increases were much lower than those caused by antibiotics. With the exception of cefotaxime, the six antibiotics increased the Stx toxin level and increased the stx2 gene mRNA level. With the exceptions of cefotaxime and tetracycline, the antibiotics increased the SOS induction response. These results suggest that TCMs may have advantages compared with antibiotics, when treating E. coli O157:H7; TCMs did not greatly increase Stx toxin production and release.
authors: State Key Laboratory of Microbial Technology, School of Life Sciences, Shandong University, 27 Shanda Nanlu, Jinan 250100, China.
authors: Mei Ling
authors: ML
authors: Chen
authors: Zhao
authors: Z
authors: Hao
authors: Yuan
authors: Y
authors: Tian
authors: Qi Yao
authors: QY
authors: Zhang
authors: Pei Ji
authors: PJ
authors: Gao
authors: Jian Ling
authors: JL
authors: Jin
references: PLoS Biol. 2005 Jul;3(7):e255
references: 16000023
references: Am J Chin Med. 2007;35(6):1047-60
references: 18186590
references: Chem Biol Interact. 2006 Mar 10;160(1):1-40
references: 16430879
references: Emerg Infect Dis. 2005 Apr;11(4):603-9
references: 15829201
references: Microbiology (Reading). 2008 Jan;154(Pt 1):176-186
references: 18174136
references: Antimicrob Agents Chemother. 2012 Jun;56(6):3277-82
references: 22391549
references: Microb Pathog. 2011 Dec;51(6):466-70
references: 21864671
references: Indian J Med Res. 2011 Feb;133:232-9
references: 21415500
references: Chem Biol Interact. 2000 Dec 1;129(1-2):113-39
references: 11154738
references: Infect Immun. 1976 Dec;14(6):1276-83
references: 12111
references: Can J Microbiol. 2009 Feb;55(2):146-53
references: 19295647
references: Int J Biol Sci. 2008 Sep 23;4(6):338-44
references: 18825275
references: Bioorg Med Chem Lett. 2010 Jan 1;20(1):56-9
references: 19959361
references: Mutat Res. 1993 Oct;297(3):235-79
references: 7692273
references: Lancet. 1996 Sep 21;348(9030):831-2
references: 8814014
references: Pediatr Nephrol. 2003 Feb;18(2):92-6
references: 12579394
references: Infect Immun. 1998 Aug;66(8):3810-7
references: 9673266
references: N Engl J Med. 2000 Jun 29;342(26):1930-6
references: 10874060
references: Proc Natl Acad Sci U S A. 1988 Mar;85(6):1811-5
references: 3279417
references: Foodborne Pathog Dis. 2010 Jan;7(1):85-90
references: 19785532
references: Eur J Med Chem. 2009 Jul;44(7):2936-43
references: 19152987
references: J Antimicrob Chemother. 2001 Jul;48 Suppl 1:5-16
references: 11420333
references: Microbiology (Reading). 2000 May;146 ( Pt 5):1085-1090
references: 10832635
references: Mutat Res. 1985 Oct;147(5):219-29
references: 3900709
references: Andrologia. 2011 Oct;43(5):321-6
references: 21806651
references: PLoS One. 2012;7(4):e36144
references: 22558360
references: Emerg Infect Dis. 2000 Sep-Oct;6(5):458-65
references: 10998375
references: Methods. 2001 Dec;25(4):402-8
references: 11846609
references: J Med Chem. 2004 Oct 21;47(22):5555-66
references: 15481991
references: PLoS One. 2012;7(7):e41628
references: 22848550
references: Zhongguo Zhong Yao Za Zhi. 2006 Jun;31(12):1015-7
references: 17048654
references: BMC Complement Altern Med. 2009 Nov 15;9:43
references: 19912670
references: N Engl J Med. 2011 Nov 10;365(19):1771-80
references: 21696328
references: Evid Based Complement Alternat Med. 2012;2012:608284
references: 22536288
references: J Med Microbiol. 2009 Jul;58(Pt 7):971-973
references: 19498201
references: Pediatr Int. 1999 Apr;41(2):228-32
references: 10221035
references: Wei Sheng Wu Xue Bao. 2011 Mar;51(3):393-401
references: 21604554
references: Antimicrob Agents Chemother. 2002 Nov;46(11):3478-83
references: 12384353
references: J Bacteriol. 2003 Jun;185(12):3596-605
references: 12775697
references: J Vet Med Sci. 2004 Aug;66(8):899-903
references: 15353838
references: Antimicrob Agents Chemother. 2007 Aug;51(8):2837-41
references: 17526759
references: Antimicrob Agents Chemother. 2010 Sep;54(9):3790-8
references: 20585113
references: Int J Food Microbiol. 2012 Jan 3;152(1-2):19-30
references: 22071287