title: New horizons in platelets flow cytometry.
abstract: Platelet flow cytometry is an emerging tool in diagnostic and therapeutic hematology. It is eminently suited to study the expression of platelet surface receptors both qualitatively as well as quantitatively. It can serve as a useful marker for the documentation of in vivo platelet activation, and thus, fore-warn the risk of thromboembolism in patients with diabetes mellitus, coronary syndromes, peripheral vascular diseases, and pre-eclampsia. This technique can also be extended to study and compare the effect of various antiplatelet drugs on the level of activation of platelets and to establish any dose-effect relationship of these drugs. Topographical localization of platelet granules and study of platelet-platelet and platelet-leukocyte interaction is also possible by this procedure. All these parameters serve as pointers towards the presence of activated platelets in the circulation with its thromboembolic consequences. This is a simple reliable and cost effective technique which has a wide application in the diagnosis of various inherited and acquired platelet disorders. Study of platelet cluster of differentiation (CD) markers in various inherited disorders i.e. Bernard Soulier's disease, von Willebrand disease, Glanzman's disease, and Grey platelet syndrome may help categories the molecular lesions in these oft under-studied disorders.
authors: Baqai Institute of Hematology, Baqai Medical University, 51 Deh Tor Gadap Road, Super Highway, Near Toll Plaza, P.O Box 2407, P. Code 74600, Karachi, Pakistan.
authors: Muhammad
authors: M
authors: Saboor
authors: Moinuddin
authors: M
authors: Moinuddin
authors: Samina
authors: S
authors: Ilyas
keywords: CD62; CD63; flow cytometry; platelets
references: Proc Natl Acad Sci U S A. 1996 Oct 15;93(21):11877-82
references: 8876231
references: Am J Hematol. 1993 Oct;44(2):106-11
references: 8266914
references: J Lab Clin Med. 2000 Mar;135(3):247-55
references: 10711863
references: Br J Haematol. 1993 Jul;84(3):527-9
references: 7692932
references: Cardiovasc Revasc Med. 2011 Sep-Oct;12(5):312-22
references: 21036109
references: J Med Virol. 2001 Feb;63(2):143-9
references: 11170051
references: Circulation. 2001 Sep 25;104(13):1533-7
references: 11571248
references: Clin Lab Sci. 1998 Nov-Dec;11(6):365-72
references: 10345505
references: J Biol Chem. 1985 Sep 15;260(20):11107-14
references: 2411729
references: Curr Opin Hematol. 1999 Sep;6(5):342-8
references: 10468151
references: Ann Med. 2009;41(1):45-51
references: 18618353
references: J Thromb Thrombolysis. 2010 Jul;30(1):79-83
references: 19866345
references: Neurol Neurochir Pol. 2000 Sep-Oct;34(5):853-64
references: 11253475
references: Ann Med. 2005;37(1):61-6
references: 15902848
references: Semin Hematol. 2001 Apr;38(2):160-8
references: 11309697
references: Thromb Haemost. 1991 May 6;65(5):467-73
references: 1871706
references: Platelets. 2000 May;11(3):137-50
references: 10938890
references: Platelets. 2003 Aug;14(5):287-94
references: 12944245
references: Hamostaseologie. 2009 Jan;29(1):25-31
references: 19151842
references: Blood. 1987 Sep;70(3):838-45
references: 3620703
references: Platelets. 2004 Feb;15(1):3-7
references: 14985170
references: Thromb Res. 2000 Nov 1;100(3):161-6
references: 11108903
references: Haematologica. 1995 Jan-Feb;80(1):69-81
references: 7758996
references: Clin Chem. 2006 Apr;52(4):657-64
references: 16439610
references: Stroke. 2002 Nov;33(11):2570-4
references: 12411644
references: Platelets. 2006 May;17(3):170-7
references: 16702044
references: Semin Thromb Hemost. 2004 Oct;30(5):501-11
references: 15497093
references: Cytometry B Clin Cytom. 2008 Jan;74(1):30-9
references: 17630652
references: Semin Thromb Hemost. 2005;31(4):393-9
references: 16149015
references: Clin Lab Haematol. 2000 Dec;22(6):359-63
references: 11318803
references: Thromb Haemost. 1998 May;79(5):885-96
references: 9609215
references: Thromb Res. 2009 Jun;124(2):213-8
references: 19233449