title: Development of a quaternized chitosan with enhanced antibacterial efficacy.
abstract: The antibacterial activity of a water-soluble chitosan derivative prepared by chemical modification to quaternary ammonium compound N,N,N-trimethylchitosan (TC) was investigated against four selected waterborne pathogens: Aeromonas hydrophila ATCC 35654, Yersinia enterocolitica ATCC 9610, Listeria monocytogenes ATCC 19111 and Escherichia coli O157:H7 ATCC 32150. An inactivation of 4 log CFU/ml of all waterborne pathogens was noted for the quaternized chitosan as compared with chitosan over a short contact time (30 min) and low dosage (4.5 ppm) at ambient temperature. A marked increase in glucose level, protein content and lactate dehydrogenase (LDH) activity was observed concurrently in the cell supernatant to be a major bactericidal mechanism. The results suggest that the TC derivative may be a promising commercial substitute for acid-soluble chitosan for rapid and effective disinfection of water.
authors: Department of Biotechnology and Environmental Sciences, Thapar University, Punjab, India.
authors: Gurpreet Kaur
authors: GK
authors: Khaira
authors: Rashmi
authors: R
authors: Kumariya
authors: Manmohan
authors: M
authors: Chibber
authors: Moushumi
authors: M
authors: Ghosh
mesh_terms: D016980:Aeromonas hydrophila / Q000187:drug effects*; D000704:Analysis of Variance; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D048271:Chitosan / Q000737:chemistry / Q000494:pharmacology*; D004926:Escherichia coli / Q000187:drug effects*; D005428:Flocculation Tests; D008089:Listeria monocytogenes / Q000187:drug effects*; D009682:Magnetic Resonance Spectroscopy; D008826:Microbial Sensitivity Tests; D000644:Quaternary Ammonium Compounds / Q000494:pharmacology; D014871:Water Microbiology; D015008:Yersinia enterocolitica / Q000187:drug effects*
chemical_list: D000900:Anti-Bacterial Agents; D000644:Quaternary Ammonium Compounds; D048271:Chitosan