title: Thermosensitive macroporous cryogels functionalized with bioactive chitosan/bemiparin nanoparticles.
abstract: Thermosensitive macroporous scaffolds of poly(N-isopropylacrylamide) (polyNIPA) loaded with chitosan/bemiparin nanoparticles are prepared by the free radical polymerization in cryogenic conditions. Chitosan/bemiparin nanoparticles of 102 ± 6.5 nm diameter are prepared by complex coacervation and loaded into polyNIPA cryogels. SEM image reveal the highly porous structure of cryogels and the integration of nanoparticles into the macroporous system. Volume phase transition temperature (VPT) and total freezing water content of cryogels are established by differential scanning calorimetry, and their porosity is determined by image-NMR. Swelling of cryogels (above and below the VPT) is highly dependent on nanoparticles concentration. In vitro release profile of bemiparin from cryogel is highly modulated by the presence of chitosan. Bemiparin released from nanoparticles preserves its biological activity, as shown by the BaF32 cell proliferation assay. Cryogels are not cytotoxic for the human fibroblast cells and present excellent properties for application on tissue engineering and controlled release of heparin.
authors: Centro de Biomateriales, Universidad de La Habana, 10400, Havana, Cuba.
authors: Hazel
authors: H
authors: Peniche
authors: Felisa
authors: F
authors: Reyes-Ortega
authors: María R
authors: MR
authors: Aguilar
authors: Gema
authors: G
authors: Rodríguez
authors: Cristina
authors: C
authors: Abradelo
authors: Luis
authors: L
authors: García-Fernández
authors: Carlos
authors: C
authors: Peniche
authors: Julio
authors: J
authors: San Román
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry* / Q000494:pharmacology; D001402:B-Lymphocytes / Q000166:cytology / Q000187:drug effects; D001672:Biocompatible Materials; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D048271:Chitosan / Q000737:chemistry*; D059985:Cryogels; D003692:Delayed-Action Preparations / Q000737:chemistry* / Q000494:pharmacology; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects; D006495:Heparin, Low-Molecular-Weight / Q000737:chemistry* / Q000494:pharmacology; D006801:Humans; D007700:Kinetics; D008855:Microscopy, Electron, Scanning; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D058105:Polymerization; D016062:Porosity; D013696:Temperature; D023822:Tissue Engineering; D054457:Tissue Scaffolds
chemical_list: D000180:Acrylic Resins; D001672:Biocompatible Materials; D059985:Cryogels; D003692:Delayed-Action Preparations; D006495:Heparin, Low-Molecular-Weight; C052970:poly-N-isopropylacrylamide; D048271:Chitosan; C411345:bemiparin
keywords: bioactive bemiparin nanoparticles; image-NMR microstructure characterization; macroporous polymeric systems; thermosensitive cryogels