title: Peptide functionalized gold nanoparticles for colorimetric detection of matrilysin (MMP-7) activity.
abstract: A peptide with two cleavage sites for MMP-7 has been synthesized and immobilized on gold nanoparticles (AuNPs) through a cysteine residue. Digestion of the peptide by MMP-7 decreases its size and net charge, which leads to the aggregation of the AuNPs. The color shift caused by aggregation enables a direct and quantitative measurement of the concentration and activity of MMP-7 with an estimated limit of detection of ∼5 nM (0.1 μg mL(-1)).
authors: Centre for Biomimetic Sensor Science, Nanyang Technological University, 50 Nanyang Drive, Singapore 637553, Singapore. bliedberg@ntu.edu.sg.
authors: Peng
authors: P
authors: Chen
authors: Robert
authors: R
authors: Selegård
authors: Daniel
authors: D
authors: Aili
authors: Bo
authors: B
authors: Liedberg
mesh_terms: D000595:Amino Acid Sequence; D003124:Colorimetry; D003545:Cysteine / Q000737:chemistry; D006046:Gold / Q000737:chemistry*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D055767:Immobilized Proteins / Q000737:chemistry / Q000378:metabolism; D020783:Matrix Metalloproteinase 7 / Q000032:analysis*; D053768:Metal Nanoparticles / Q000737:chemistry*; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry* / Q000378:metabolism; D012463:Saliva / Q000201:enzymology; D012468:Salivary Gland Neoplasms / Q000201:enzymology / Q000473:pathology
chemical_list: D055767:Immobilized Proteins; D010455:Peptides; D006046:Gold; D020783:Matrix Metalloproteinase 7; D003545:Cysteine