title: [Screening and identification of glycoproteins from Campylobacter jejuni NCTC 11168 based on specific affinity between lectins and glycoproteins].
abstract: OBJECTIVE
  To screen glycoproteins from Campylobacter jejuni NCTC 11168.
  
  
  METHODS
  We introduced a screening strategy based on specific affinity between lectins and glycoproteins. First, the specific affinity between Soybean Agglutinin (SBA) and glycoproteins in C. jejuni was confirmed by western blotting. Then, magnetic beads coating with Soybean Agglutinin (SBA) were used to capture the putative glycoproteins. Putative glycoproteins were competitively eluted by N-acetylgalactosamine and separated by two-dimensional gel electrophoresis. Spots in gels were further analyzed by mass spectrometry.
  
  
  RESULTS
  A total of 22 proteins were identified by mass spectrometric analysis, of which 5 have ever been identified to be glycoproteins. Others including Cj0633 have not been proved to be glycosylated so far.
  
  
  CONCLUSIONS
  The method could be used to screen glycoproteins in bacteria.
authors: Medical University of People's Liberation Army, Beijing 100853, China. guowangbai@163.com
authors: Guowang
authors: G
authors: Bai
authors: Gang
authors: G
authors: Yu
authors: Xiankai
authors: X
authors: Liu
authors: Erling
authors: E
authors: Feng
authors: Ming
authors: M
authors: Zeng
authors: Hengliang
authors: H
authors: Wang
authors: Li
authors: L
authors: Zhu
mesh_terms: D016123:Campylobacter jejuni / Q000378:metabolism*; D006023:Glycoproteins / Q000737:chemistry / Q000378:metabolism*; D037121:Plant Lectins / Q000378:metabolism*; D011485:Protein Binding; D020543:Proteome; D040901:Proteomics / Q000379:methods; D030262:Soybean Proteins / Q000378:metabolism*; D010835:Phytohemagglutinins
chemical_list: D006023:Glycoproteins; D037121:Plant Lectins; D020543:Proteome; D030262:Soybean Proteins; C028824:soybean lectin; D010835:Phytohemagglutinins