title: Single nucleotide polymorphism of CD40 in the 5'-untranslated region is associated with ischemic stroke.
abstract: OBJECTIVE
  Ischemic stroke is influenced by both environmental and genetic factors. The CD40/CD40L system is related to proinflammatory and prothrombogenic responses, which are involved in the pathophysiology of ischemic stroke. The aim of this study was to evaluate association between the CD40 -1C/T single nucleotide polymorphism (SNP) and ischemic stroke in a Chinese population.
  
  
  METHODS
  We conducted a case-control study including 286 ischemic stroke patients and 336 controls. CD40 -1C/T SNP was genotyped using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and DNA sequencing methods, and evaluated its relevance to ischemic stroke susceptibility.
  
  
  RESULTS
  Significantly increased ischemic stroke risk was found to be associated with the T allele of CD40 -1C/T (OR=1.273, 95% CI=1.016-1.594). The frequencies of CT and TT/CT genotypes of CD40 -1C/T in ischemic stroke patients were significantly higher than those of controls, respectively (for CT: OR=2.350, 95% CI=1.601-3.449; for TT/CT: OR=2.148, 95% CI=1.479-3.119). And, similar results were obtained after adjusting non-matched variables. We found that the frequency of carried T genotypes (TT and TT/CT) was significantly increased in patients with history of stroke compared with patients without (for TT: OR=6.538, 95%CI=1.655-25.833; for TT/CT: OR=3.469, 95%CI=1.031-11.670), respectively.
  
  
  CONCLUSIONS
  The findings suggested that the CD40 -1C/T polymorphism might contribute to the susceptibility to ischemic stroke in the Chinese population, and might be associated with history of previous stroke.
authors: Department of Neurology, Affiliated Hospital of North Sichuan Medical College, Nanchong 637000, PR China.
authors: Ying
authors: Y
authors: Ma
authors: Shun-Xian
authors: SX
authors: Wang
authors: Yun
authors: Y
authors: Liu
authors: Guo-Guang
authors: GG
authors: Peng
authors: Xiao-Ming
authors: XM
authors: Wang
authors: Bo
authors: B
authors: Zhang
authors: Bi-Hua
authors: BH
authors: Wu
authors: Ju-Ming
authors: JM
authors: Yu
mesh_terms: D020121:5' Untranslated Regions; D000368:Aged; D002545:Brain Ischemia / Q000150:complications / Q000235:genetics*; D019013:CD40 Antigens / Q000235:genetics*; D016022:Case-Control Studies; D005260:Female; D056726:Genetic Association Studies; D006801:Humans; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D020521:Stroke / Q000209:etiology / Q000235:genetics*
chemical_list: D020121:5' Untranslated Regions; D019013:CD40 Antigens
keywords: 5′-UTR; 5′-untranslated region; CD40; CD40 ligand; CD40L; CE; CHD; CI; CNS; CT; Genetic susceptibility; HWE; Hardy–Weinberg equilibrium; ICD-9-CM; International Classification of Diseases, 9th Revision, Clinical Modification; Ischemic stroke; LAA; LD; MRI; NIHSS; National Institutes of Health Stroke Scale; OR; PCR-RFLP; SNP; SVO; Single nucleotide polymorphism; TNFR; TOAST; Trial of Org10172 in Acute Stroke Treatment; cardioembolism; central nervous system; computed tomography; confidence intervals; coronary heart disease; large artery atherosclerosis; linkage disequilibrium; magnetic resonance imaging; odds ratio; polymerase chain reaction-restriction fragment length polymorphism; sCD40; single nucleotide polymorphism; small vessel occlusion; soluble CD40; tumor necrosis factor receptor