title: Fluorescent proteins in cellular organelles: serious pitfalls and some solutions.
abstract: Fluorescent proteins (FPs) have been powerful tools for cell biologists for over 15 years. The large variety of FPs available rarely comes with an instruction manual or a warning label. The potential pitfalls of the use of FPs in cellular organelles represent a significant concern for investigators. FPs generally did not evolve in the often distinctive physicochemical environments of subcellular organelles. In organelles, FPs can misfold, go dark, and even distort organelle morphology. In this minireview, we describe the issues associated with FPs in organelles and provide solutions to enable investigators to better exploit FP technology in cells.
authors: Department of Anatomy and Structural Biology, Albert Einstein College of Medicine , Bronx, New York.
authors: Lindsey M
authors: LM
authors: Costantini
authors: Erik Lee
authors: EL
authors: Snapp
mesh_terms: D000818:Animals; D004220:Disulfides; D005453:Fluorescence; D006031:Glycosylation; D006801:Humans; D008164:Luminescent Proteins / Q000032:analysis* / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D008967:Molecular Biology / Q000379:methods; D015388:Organelles / Q000378:metabolism*; D010084:Oxidation-Reduction; D011487:Protein Conformation; D011994:Recombinant Proteins / Q000032:analysis / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
chemical_list: D004220:Disulfides; D008164:Luminescent Proteins; D011994:Recombinant Proteins
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