title: Superoxide-mediated protection of Escherichia coli from antimicrobials.
abstract: Antimicrobial lethality is promoted by reactive oxygen species (ROS), such as superoxide, peroxide, and hydroxyl radical. Pretreatment with subinhibitory concentrations of plumbagin or paraquat, metabolic generators of superoxide, paradoxically reduced killing for oxolinic acid, kanamycin, and ampicillin. These pretreatments also reduced an oxolinic acid-mediated ROS surge. Defects in SoxS MarA or AcrB eliminated plumbagin- and paraquat-mediated MIC increases but maintained protection from killing. Thus, superoxide has both protective and detrimental roles in response to antimicrobial stress.
authors: Public Health Research Institute, New Jersey Medical School, Rutgers Biomedical and Health Sciences, Newark, New Jersey, USA.
authors: Michael
authors: M
authors: Mosel
authors: Liping
authors: L
authors: Li
authors: Karl
authors: K
authors: Drlica
authors: Xilin
authors: X
authors: Zhao
mesh_terms: D000667:Ampicillin / Q000494:pharmacology*; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D024881:Drug Resistance, Bacterial / Q000235:genetics; D048168:Escherichia coli K12 / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D007612:Kanamycin / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D027425:ATP-Binding Cassette, Sub-Family C Proteins / Q000235:genetics / Q000378:metabolism; D009285:Naphthoquinones / Q000737:chemistry; D010093:Oxolinic Acid / Q000494:pharmacology*; D010269:Paraquat / Q000737:chemistry; D013481:Superoxides / Q000737:chemistry / Q000494:pharmacology*; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism
chemical_list: D000667:Ampicillin; D000900:Anti-Bacterial Agents; D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; D007612:Kanamycin; D027425:ATP-Binding Cassette, Sub-Family C Proteins; D009285:Naphthoquinones; D010093:Oxolinic Acid; D010269:Paraquat; D013481:Superoxides; D015534:Trans-Activators; C083496:AcrB protein, E coli; C080002:MarA protein, E coli; C070725:SoxS protein, E coli; C014758:plumbagin
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