title: Effects of sex and season in haematological parameters and cellular composition of spleen and head kidney of pejerrey (Odontesthes bonariensis).
abstract: Phylogenetic diversity in fish determines high interspecific variability in morphology as well as in physiological parameters. Moreover, several haematological variables and the organ composition of haemolymphopoietic sites may vary according to sex or season. The aim of this study was to establish the haematological parameters and the cellular composition of haemolymphopoietic organs in Odontesthes bonariensis, a commercially valuable fish species in Argentina, and also to determine gender or seasonal variations. Haematocrit exhibited the highest value in summer, while haemoglobin concentration was greater in summer and autumn. Erythrocyte count was higher in spring than autumn and winter, but did not differ with summer. The increase in these variables in seasons with higher water temperatures might be a compensatory mechanism to compensate the lower level of oxygen in the environment. Leucocyte formula and blast haemolymphopoietic cells in spleen and head kidney also showed annual variations since cells related to specific immune response, i.e., lymphocytes and thrombocytes, decrease in winter, whereas cells of the non-specific immune pathways, such as granulocyte, rise. The elevation of a particular type of circulating leucocyte was preceded by an increase in values of its precursor in blood in the previous season. Both, spleen and head kidney were active in haemolymphopoiesis, although with some differences in their activity during different seasons. Males showed higher values of circulating lymphoblasts and granulocytes than females, whereas females exhibited higher values of thrombocytes. This study corroborates the high interspecific variations in haematological parameters in fish that underlines the needing of basic studies in order to assess fish health status in new promising species for aquaculture.
authors: Cátedra de Histología y Embriología, Facultad de Ciencias Veterinarias, Universidad Nacional de Rosario, Bv. Ovidio Lagos y Ruta 33, 2170, Casilda, Argentina, fviglian@fveter.unr.edu.ar.
authors: Fabricio A
authors: FA
authors: Vigliano
authors: Adolfo M
authors: AM
authors: Araujo
authors: Andrés J
authors: AJ
authors: Marcaccini
authors: María V
authors: MV
authors: Marengo
authors: Eliana
authors: E
authors: Cattaneo
authors: Carina
authors: C
authors: Peirone
authors: Lucrecia G M
authors: LG
authors: Dasso
mesh_terms: D000818:Animals; D001118:Argentina; D001772:Blood Cell Count; D005260:Female; D029941:Fish Proteins / Q000097:blood; D005398:Fisheries; D005399:Fishes / Q000033:anatomy & histology* / Q000097:blood*; D060226:Head Kidney / Q000166:cytology*; D006400:Hematocrit; D006454:Hemoglobins / Q000378:metabolism; D008297:Male; D012016:Reference Values; D012621:Seasons; D012727:Sex Characteristics; D013045:Species Specificity; D013154:Spleen / Q000166:cytology*
chemical_list: D029941:Fish Proteins; D006454:Hemoglobins
references: Fish Shellfish Immunol. 2008 Dec;25(6):791-9
references: 19004642
references: Dev Biol Stand. 1997;90:33-43
references: 9270832
references: Anat Histol Embryol. 2006 Apr;35(2):76-83
references: 16542171
references: Environ Res. 2006 May;101(1):74-80
references: 16388797
references: Braz J Biol. 2003 Aug;63(3):517-25
references: 14758711
references: Fish Physiol Biochem. 2013 Feb;39(1):71-4
references: 22580925
references: Biocell. 2006 Apr;30(1):157-62
references: 16845841
references: Comp Immunol Microbiol Infect Dis. 1995 Feb;18(2):105-13
references: 7621666
references: Int Rev Cytol. 2002;220:35-92
references: 12224552
references: J Exp Zool A Comp Exp Biol. 2005 Jun 1;303(6):504-14
references: 15880765
references: Vet Immunol Immunopathol. 1986 Jun;12(1-4):213-21
references: 3765343
references: Comp Biochem Physiol A Mol Integr Physiol. 2012 Sep;163(1):47-55
references: 22583672
references: J Fish Biol. 2011 Mar;78(3):901-11
references: 21366580
references: Tissue Cell. 2007 Jun;39(3):151-60
references: 17477946
references: Fish Shellfish Immunol. 2002 Apr;12(4):303-34
references: 12049168
references: Acta Vet Hung. 2002;50(4):459-67
references: 12498150
references: Fish Physiol Biochem. 2008 Jun;34(2):169-74
references: 18649034
references: Braz J Biol. 2002 Nov;62(4A):633-40
references: 12659013
references: J Fish Dis. 2004 Jun;27(6):359-68
references: 15189376
references: J Fish Dis. 2012 Feb;35(2):83-108
references: 22233511
references: Am J Physiol Regul Integr Comp Physiol. 2009 Oct;297(4):R1136-43
references: 19675278
references: Fish Shellfish Immunol. 2006 Oct;21(4):467-71
references: 16533606
references: Braz J Biol. 2011 Aug;71(3):719-25
references: 21881796