title: Generation and characterization of epiblast stem cells from blastocyst-stage mouse embryos.
abstract: Mouse epiblast stem cells (EpiSCs) are pluripotent embryonic cells that can be used to interrogate developmental transitions that occur during gastrulation. EpiSCs can also be robustly differentiated into functional somatic and germ cell derivatives making them a useful tool for studying development and regenerative medicine. Typically, mouse EpiSCs are isolated from the early postimplantation epiblast around 5.5 days post coitum (dpc). This chapter describes the methods for isolation of mouse EpiSCs from preimplantation blastocyst-stage mouse embryos (3.5 dpc). This technique enables the routine ability to derive EpiSC lines as it is much less labor intensive than isolation of EpiSCs from the postimplantation epiblast. We also detail relevant assays used to characterize new EpiSC lines and distinguish them from mouse embryonic stem cells.
authors: Department of Genetics and Genome Sciences, Case Western Reserve, University School of Medicine, Cleveland, OH, USA.
authors: Daniel C
authors: DC
authors: Factor
authors: Fadi J
authors: FJ
authors: Najm
authors: Paul J
authors: PJ
authors: Tesar
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000166:cytology*; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation; D002460:Cell Line; D053595:Embryonic Stem Cells / Q000166:cytology*; D054262:Gastrulation / Q000235:genetics; D005854:Germ Cells / Q000166:cytology; D005855:Germ Layers / Q000166:cytology*; D051379:Mice