title: [In vitro spermatogenesis… new horizon to restore fertility?].
abstract: The survival of the young boy after cancer has considerably progressed in recent years due to the efficiency of chemo/radiotherapy against the tumor cells. However, this treatment causes adverse effects on healthy tissues, including fertility. Freezing testicular tissue before highly gonadotoxic treatment is a prerequisite for preserving fertility in prepubertal boys that do not produce sperm yet. But which strategy proposes to restore fertility from frozen-thawed testicular tissue? One potential solution would be to consider an in vitro maturation of spermatogonial stem cells. In this article we trace the chronological development of in vitro spermatogenesis that resulted in mouse sperm production in vitro and give an overview of new challenges for the future.
authors: CECOS, EA4308 « gamétogenèse et qualité du gamète », IRIB, laboratoire de biologie de la reproduction, université de Rouen, hôpitaux de Rouen, CHU Charles-Nicolle, 1, rue de Germont, 76031 Rouen cedex, France. brahim.arkoun@etu.univ-rouen.fr
authors: B
authors: B
authors: Arkoun
authors: L
authors: L
authors: Dumont
authors: J-P
authors: JP
authors: Milazzo
authors: A
authors: A
authors: Bironneau
authors: A
authors: A
authors: Way
authors: B
authors: B
authors: Macé
authors: N
authors: N
authors: Rives
mesh_terms: D053687:Adult Stem Cells / Q000502:physiology*; D000818:Animals; D059247:Fertility Preservation / Q000379:methods*; D049673:History, 20th Century; D049674:History, 21st Century; D008297:Male; D051379:Mice; D009924:Organ Culture Techniques / Q000266:history / Q000379:methods; D013091:Spermatogenesis
keywords: In vitro spermatogenesis; Spermatides; Spermatids; Spermatogenèse in vitro; Spermatogonia; Spermatogonies; Spermatozoa; Spermatozoïdes