title: Facile detection of photogenerated reactive oxygen species in TiO2 nanoparticles suspension using colorimetric probe-assisted spectrometric method.
abstract: This present study reports that reactive oxygen species (ROS), which mainly consist of hydroxyl (OH) and superoxide (O2(-)) radicals generated by the photocatalysis of TiO2 nanoparticles (TiO2 NPs) in suspension, can be quantitatively measured by the colorimetric probe-assisted spectrometric method without any interference by the TiO2 NPs. The interference effect of the TiO2 NPs with UV-vis spectroscopy was excluded by the selective separation of p-nitrosodimethylaniline (RNO) and tetrazolium salt (XTT) (probe compounds for OH and O2(-), respectively) from the TiO2 NPs in the suspension by centrifugation at a specified ionic strength adjusted with phosphate buffer (KH2PO4/NaOH, PB) solution. This colorimetric probe-assisted spectrometric method was also successfully applied to measure the steady-state production of photogenerated ROS in a TiO2 NPs suspension.
authors: WCU Program of Chemical Convergence for Energy & Environment, School of Chemical and Biological Engineering, Seoul National University (SNU), Gwanak-gu, Daehak-dong, Seoul 151-742, Republic of Korea; School of Chemical and Biological Engineering, College of Engineering, Institute of Chemical Process, Seoul National University (SNU), Daehak-dong, Gwanak-gu, Seoul 151-742, Republic of Korea.
authors: Choonsoo
authors: C
authors: Kim
authors: Hee-jin
authors: HJ
authors: Park
authors: Seoeun
authors: S
authors: Cha
authors: Jeyong
authors: J
authors: Yoon
mesh_terms: D003124:Colorimetry; D017665:Hydroxyl Radical / Q000737:chemistry; D053768:Metal Nanoparticles / Q000032:analysis* / Q000737:chemistry; D008956:Models, Chemical; D055668:Photochemical Processes; D017382:Reactive Oxygen Species / Q000737:chemistry*; D013481:Superoxides / Q000737:chemistry; D014025:Titanium / Q000032:analysis* / Q000737:chemistry
chemical_list: D017382:Reactive Oxygen Species; D013481:Superoxides; C009495:titanium dioxide; D017665:Hydroxyl Radical; D014025:Titanium
keywords: Nanoparticle interference; Selective seperation; Spectrophotometric ROS detection; TiO(2) nanoparticles suspension