title: Radioprotective effect of vitamin E in parotid glands: a morphometric analysis in rats.
abstract: The aim of this study was to evaluate the radioprotective effect of vitamin E on rat parotid glands by morphometric analysis. Sixty male rats were divided into 5 groups (n=6): control, in which animals received olive oil solution; olive oil/irradiated, in which animals received olive oil and were irradiated with a dose of 15 Gy of gamma radiation; irradiated, in which animals were irradiated with a dose of 15 Gy gamma radiation; vitamin E, which received α-tocopherol acetate solution; vitamin E/irradiated, which received α-tocopherol acetate solution before irradiation with a dose of 15 Gy gamma rays. Half of the animals were euthanized at 8 h, and the remaining at 30 days after irradiation. Both parotid glands were surgically removed and morphometric analysis of acinar cells was performed. Data were subjected to two-way ANOVA and Tukey's test (α=0.05). Morphometric analysis showed a significant reduction in the number of parotid acinar cells at 30 days in olive oil/irradiated and irradiated groups. In groups evaluated over time a significant reduction was shown at 30 days in olive oil/irradiated and irradiated groups, indicating that ionizing radiation caused tissue damage. The vitamin E/irradiated group presented more acinar cells than the irradiated group, but no statistically significant difference was observed (p>0.05). In conclusion, vitamin E seems to have failed as a radioprotective agent on acinar cells in rat parotid glands.
authors: Department of Oral Diagnosis, Piracicaba Dental School, University of Campinas, Piracicaba, SP, Brazil.
authors: Carolina Cintra
authors: CC
authors: Gomes
authors: Flávia Maria de Moraes
authors: FM
authors: Ramos-Perez
authors: Danyel Elias da Cruz
authors: DE
authors: Perez
authors: Pedro Duarte
authors: PD
authors: Novaes
authors: Frab Norberto
authors: FN
authors: Bóscolo
authors: Solange Maria de
authors: SM
authors: Almeida
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000627:therapeutic use*; D001284:Atrophy; D005720:Gamma Rays; D008297:Male; D009929:Organ Size; D010306:Parotid Gland / Q000187:drug effects / Q000473:pathology / Q000528:radiation effects*; D011829:Radiation Dosage; D011837:Radiation-Protective Agents / Q000627:therapeutic use*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D018987:Salivary Ducts / Q000187:drug effects / Q000473:pathology / Q000528:radiation effects; D013997:Time Factors; D014810:Vitamin E / Q000627:therapeutic use*
chemical_list: D000975:Antioxidants; D011837:Radiation-Protective Agents; D014810:Vitamin E