title: Electrochemical immunoassay for procalcitonin antigen detection based on signal amplification strategy of multiple nanocomposites.
abstract: Procalcitonin, as a medium of inflammation, has become a new marker of the identification of severe bacterial infections in recent years and has received high attention due to its most ideal diagnostic indicators of specificity with major types of organism systemic inflammation of bacterial infection in the early stages. Thus, a novel method for the determination of procalcitonin (PCT) was developed based on a sandwich-type electrochemical immunosensor, which combined a simple immunosensor array as well as an effectively designed trace tag. The immunosensor was fabricated by layer-by-layer coating graphene (GC), carbon nanotubes (MWCNTs), chitosan (CS), glutaraldehyde (GA) composite on the working electrode, which can increase the electronic transfer rate and improve the surface area to capture a large number of primary antibodies (Ab1). The trace tag was prepared by loading high-content signal horseradish peroxidase labeled secondary PCT antibody (HRP-Ab2) with AuNPs, which were coated with mesoporous silica nanoparticles (MCM-41) through thionine linking. In comparison with conventional methods, the proposed immunosensor for PCT provided a better linear response range from 0.01 to 350 ng/mL and a lower limit of detection (LOD) of 0.5 pg/mL under optimal experimental conditions. In addition, the immunosensor exhibited convenience, low cost, rapidity, good specificity, acceptable stability and reproducibility. Moreover, satisfactory results were obtained for the determination of PCT in real human serum samples, indicating that the developed immunoassay has the potential to find application in clinical detection of PCT and other tumor markers as an alternative approach.
authors: School of Chemistry and Chemical Engineering, South China University of Technology, Guangzhou 510641, People's Republic of China.
authors: Yi-Shan
authors: YS
authors: Fang
authors: Hai-Ying
authors: HY
authors: Wang
authors: Li-Shi
authors: LS
authors: Wang
authors: Ju-Fang
authors: JF
authors: Wang
mesh_terms: D055910:Antibodies, Immobilized / Q000737:chemistry; D015374:Biosensing Techniques / Q000379:methods; D002116:Calcitonin / Q000097:blood*; D015740:Calcitonin Gene-Related Peptide; D048271:Chitosan / Q000737:chemistry*; D055664:Electrochemical Techniques / Q000379:methods*; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D007118:Immunoassay / Q000379:methods; D057230:Limit of Detection; D053761:Nanocomposites / Q000737:chemistry* / Q000648:ultrastructure; D037742:Nanotubes, Carbon / Q000737:chemistry / Q000648:ultrastructure; D011498:Protein Precursors / Q000097:blood*; D015203:Reproducibility of Results; D012822:Silicon Dioxide / Q000737:chemistry*
chemical_list: D055910:Antibodies, Immobilized; C000606589:CALCA protein, human; D037742:Nanotubes, Carbon; D011498:Protein Precursors; D012822:Silicon Dioxide; D006108:Graphite; D002116:Calcitonin; D048271:Chitosan; D015740:Calcitonin Gene-Related Peptide
keywords: Chitosan; Electrochemical immunosensor; Graphene; Multiwalled carbon nanotubes; Procalcitonin