title: Optimal Medium Conditions for the Detection of Cellulolytic Activity in Ganoderma lucidum.
abstract: To determine the optimal medium conditions for the detection of the cellulolytic activity in Ganoderma lucidum, we varied three media conditions: dye reagent, pH, and temperature. First, we evaluated the use of four dyes, Congo Red, Phenol Red, Remazol Brilliant Blue, and Trypan Blue. To observe the effect of pH on the chromogenic reaction, we also made and tested various media spanning acidic and alkaline pHs, ranging from 4.5 to 8.0. Furthermore, in order to research the effect of temperature on the clear zone and the fungus growing zone, we tested temperatures ranging from 15 to 35℃. On the whole, the best protocol called for Ganoderma lucidum transfer onto media containing Congo red with pH adjusted to 7.0, followed by incubation at 25℃ for 5 days. Our results will be useful to researchers who aim to study extracellular enzyme activity in Ganoderma lucidum.
authors: Department of Agricultural Environment, Gyeongbuk Agricultural Technology Administration, Daegu 702-320, Korea.
authors: Woo-Sik
authors: WS
authors: Jo
authors: Soon-Hwa
authors: SH
authors: Bae
authors: Doo-Hyun
authors: DH
authors: Cho
authors: So-Deuk
authors: SD
authors: Park
authors: Young-Bok
authors: YB
authors: Yoo
authors: Seung-Chun
authors: SC
authors: Park
keywords: Cellulolytic enzyme; Chromogenic media; Congo red; Ganoderma lucidum
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references: 24015063
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