title: Effect of hypertonic versus isotonic saline resuscitation on heme oxygenase-1 expression in visceral organs following hemorrhagic shock in rats.
abstract: To compare the early effects of hypertonic and isotonic saline resuscitation on heme oxygenase-1 (HO-1) expression in organs of rats with hemorrhagic shock. Rats were randomly divided into hypertonic saline resuscitation (HTS), normal saline resuscitation (NS) and sham groups. HO-1 mRNA, protein expression and apoptosis were evaluated in organs. In the HTS group, significant difference was noted in HO-1 protein in small intestinal mucosa and liver compared with the NS and sham groups, and in HO-1 mRNA in liver and kidney compared with the sham group. The apoptosis of small intestinal mucosa, liver, heart, and lung was significantly lower in the HTS group than that in the NS group. In this study, small volume resuscitation with HTS can efficiently up-regulate the expression level of HO-1 in small intestinal mucosa and liver, which may be one of the mechanisms alleviating organ damage.
authors: Department of Emergency Medicine, First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310003, Zhejiang China; 2. Cancer Institute, Zhejiang Tumor Hospital, Hangzhou 310022, Zhejiang China.
authors: Yuan Qiang
authors: YQ
authors: Lu
authors: Lin Hui
authors: LH
authors: Gu
authors: Jiu Kun
authors: JK
authors: Jiang
authors: Han Zhou
authors: HZ
authors: Mou
mesh_terms: D000818:Animals; D001483:Base Sequence; D001794:Blood Pressure; D017931:DNA Primers; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects*; D051547:Heme Oxygenase-1 / Q000378:metabolism*; D007421:Intestine, Small / Q000201:enzymology; D007668:Kidney / Q000201:enzymology; D008099:Liver / Q000201:enzymology; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D012151:Resuscitation / Q000379:methods*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012462:Saline Solution, Hypertonic / Q000494:pharmacology*; D012771:Shock, Hemorrhagic / Q000201:enzymology*
chemical_list: D017931:DNA Primers; D012333:RNA, Messenger; D012462:Saline Solution, Hypertonic; D051547:Heme Oxygenase-1