title: Chitosan-based nanocoatings for hypothermic storage of living cells.
abstract: The formation of ultrathin chitosan-based nanocoating on HL-60 model cells and their protective function in hypothermic storage are presented. HL-60 cells are encapsulated in ultrathin shells by adsorbing cationic and anionic chitosan derivatives in a stepwise, layer-by-layer, procedure carried out in an aqueous medium under mild conditions. The chitosan-based films are also deposited on model lipid bilayer and the interactions are studied using ellipsometry and atomic force microscopy. The cells covered with the chitosan-based films and stored at 4 °C for 24 h express viability comparable to that of the control sample incubated at 37 °C, while the unprotected cells stored under the same conditions do not show viability. It is shown that the chitosan-based shell protects HL-60 cells against damaging effect of hypothermic storage. Such nanocoatings provide protection, mechanical stability, and support the cell membrane, while ensuring penetration of small molecules such as nutrients/gases what is essential for cell viability.
authors: Faculty of Chemistry, Jagiellonian University, Ingardena 3, 30-060, Krakow, Poland.
authors: Maria
authors: M
authors: Bulwan
authors: Magdalena
authors: M
authors: Antosiak-Iwańska
authors: Ewa
authors: E
authors: Godlewska
authors: Ludomira
authors: L
authors: Granicka
authors: Szczepan
authors: S
authors: Zapotoczny
authors: Maria
authors: M
authors: Nowakowska
mesh_terms: D000327:Adsorption; D002240:Carbohydrate Sequence; D002463:Cell Membrane Permeability / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D018914:Cells, Immobilized / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D048271:Chitosan / Q000031:analogs & derivatives* / Q000138:chemical synthesis* / Q000494:pharmacology; D020099:Coated Materials, Biocompatible / Q000138:chemical synthesis* / Q000494:pharmacology; D018922:HL-60 Cells; D006801:Humans; D008051:Lipid Bilayers / Q000737:chemistry; D018625:Microscopy, Atomic Force; D008856:Microscopy, Fluorescence; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D008969:Molecular Sequence Data; D012034:Refrigeration; D014867:Water
chemical_list: D020099:Coated Materials, Biocompatible; D008051:Lipid Bilayers; D014867:Water; D048271:Chitosan
keywords: cell encapsulation; chitosan; hypothermic storage; polymer nanocoating