title: [Benzoxazole derivative B-98 ameliorates dextran sulfate sodium-induced acute murine colitis and the change of T cell profiles in acute murine colitis model].
abstract: OBJECTIVE
  The unique role of enzyme 5-lipoxygenase (5-LO) in the production of leukotrienes makes it a therapeutic target for inflammatory bowel disease (IBD). The aim of this study was to evaluate the effects of B-98, a newly synthesized benzoxazole derivatives and a novel 5-LO inhibitor, in a mouse model of IBD induced by dextran sulfate sodium (DSS).
  
  
  METHODS
  C57BL/6 mice were randomly assigned to four groups: normal control, DSS colitis (DSS+saline), low dose B-98 (DSS＋B-98 20 mg/kg) and high dose B-98 (DSS＋B-98 100 mg/kg). B-98 was administered with 3% DSS intraperitoneally. The severity of the colitis was assessed via the disease activity index (DAI), colon length, and histopathologic grading. The production of inflammatory cytokines interleukin (IL)-6 was determined by RT-PCR. Th cells were examined for the proportion of Th1 cell, Th2 cell, Th9 cell, Th17 cell and Treg cell using intracellular cytometry.
  
  
  RESULTS
  The B-98 group showed lower DAI, less shortening of the colon length and lower histopathologic grading compared with the DSS colitis group (p<0.01). The expression of IL-6 in colonic tissue was significantly lower in the B-98 groups than the DSS colitis group (p<0.05). The cellular profiles revealed that the Th1, Th9 and Th17 cells were increased in the DSS colitis group compared to the B-98 group (p<0.05).
  
  
  CONCLUSIONS
  Our results suggest that acute intestinal inflammation is reduced in the group treated with B-98 by Th1, Th9 and Th17 involved cellular immunity.
authors: Department of Internal Medicine, Ewha Womans University School of Medicine, Seoul, Korea.
authors: Eun Mi
authors: EM
authors: Song
authors: Sung Ae
authors: SA
authors: Jung
authors: Jong Soo
authors: JS
authors: Lee
authors: Seung Eun
authors: SE
authors: Kim
authors: Ki Nam
authors: KN
authors: Shim
authors: Hye Kyung
authors: HK
authors: Jung
authors: Kwon
authors: K
authors: Yoo
authors: Hae Young
authors: HY
authors: Park
mesh_terms: D000208:Acute Disease; D000818:Animals; D001094:Arachidonate 5-Lipoxygenase / Q000737:chemistry / Q000378:metabolism; D001583:Benzoxazoles / Q000737:chemistry / Q000494:pharmacology*; D003092:Colitis / Q000139:chemically induced / Q000473:pathology / Q000517:prevention & control*; D003106:Colon / Q000187:drug effects / Q000473:pathology / Q000502:physiology; D016264:Dextran Sulfate / Q000633:toxicity; D004195:Disease Models, Animal; D051858:Forkhead Transcription Factors / Q000378:metabolism; D007274:Injections, Intraperitoneal; D015850:Interleukin-6 / Q000235:genetics / Q000378:metabolism; D016859:Lipoxygenase Inhibitors / Q000737:chemistry / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D012720:Severity of Illness Index; D013601:T-Lymphocytes / Q000145:classification / Q000187:drug effects* / Q000378:metabolism
chemical_list: C000589227:B-98 compound; D001583:Benzoxazoles; D051858:Forkhead Transcription Factors; C498833:Foxp3 protein, mouse; D015850:Interleukin-6; D016859:Lipoxygenase Inhibitors; D016264:Dextran Sulfate; D001094:Arachidonate 5-Lipoxygenase