title: Transcription factor σB plays an important role in the production of extracellular membrane-derived vesicles in Listeria monocytogenes.
abstract: Gram-negative bacteria produce extracellular outer membrane vesicles (OMVs) that interact with host cells. Unlike Gram-negative bacteria, less is known about the production and role of extracellular membrane vesicles (MVs) in Gram-positive bacteria. The food-borne pathogen Listeria monocytogenes can survive under extreme environmental and energy stress conditions and the transcription factor σ(B) is involved in this survival ability. Here, we first determined the production of MVs from L. monocytogenes and evaluated whether general stress transcription factor σ(B) affected production of MVs in L. monocytogenes. L. monocytogenes secreted MVs during in vitro broth culture. The wild-type strain actively produced MVs approximately nine times more and also produced more intact shapes of MVs than those of the isogenic ΔsigB mutant. A proteomic analysis showed that 130 and 89 MV proteins were identified in the wild-type and ΔsigB mutant strains, respectively. Wild-type strain-derived MVs contained proteins regulated by σ(B) such as transporters (OpuCA and OpuCC), stress response (Kat), metabolism (LacD), translation (InfC), and cell division protein (FtsZ). Gene Ontology (GO) enrichment analysis showed that wild-type-derived MV proteins corresponded to several GO terms, including response to stress (heat, acid, and bile resistance) and extracellular polysaccharide biosynthetic process, but not the ΔsigB mutant. Internalin B (InlB) was almost three times more contained in MVs derived from the wild-type strain than in MVs derived from the ΔsigB mutant. Taken together, these results suggest that σ(B) plays a pivotal role in the production of MVs and protein profiles contained in MVs. L. monocytogenes MVs may contribute to host infection and survival ability under various stressful conditions.
authors: Department of Microbiology, School of Medicine, Kyungpook National University, Daegu, Republic of Korea.
authors: Jung Hwa
authors: JH
authors: Lee
authors: Chi-Won
authors: CW
authors: Choi
authors: Taewon
authors: T
authors: Lee
authors: Seung Il
authors: SI
authors: Kim
authors: Je-Chul
authors: JC
authors: Lee
authors: Ji-Hyun
authors: JH
authors: Shin
mesh_terms: D001426:Bacterial Proteins / Q000145:classification / Q000378:metabolism; D015153:Blotting, Western; D002853:Chromatography, Liquid; D005110:Extracellular Space / Q000378:metabolism*; D063990:Gene Ontology; D008089:Listeria monocytogenes / Q000378:metabolism* / Q000472:pathogenicity / Q000648:ultrastructure; D009154:Mutation; D022142:Secretory Vesicles / Q000378:metabolism* / Q000648:ultrastructure; D012808:Sigma Factor / Q000378:metabolism*; D021241:Spectrometry, Mass, Electrospray Ionization; D037521:Virulence Factors / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; C480945:SigB protein, Listeria monocytogenes; D012808:Sigma Factor; D037521:Virulence Factors
references: J Bacteriol. 2006 Aug;188(16):5945-57
references: 16885463
references: J Bacteriol. 1995 Jul;177(14):3998-4008
references: 7608073
references: Appl Environ Microbiol. 2003 Apr;69(4):2015-22
references: 12676677
references: Nat Genet. 2000 May;25(1):25-9
references: 10802651
references: Appl Environ Microbiol. 2001 Oct;67(10):4454-7
references: 11571142
references: PLoS One. 2011 Feb 28;6(2):e17027
references: 21386968
references: Microbiol Immunol. 2004;48(12):965-9
references: 15611613
references: Cell. 2003 Oct 3;115(1):25-35
references: 14532000
references: J Bacteriol. 2005 Apr;187(7):2286-96
references: 15774871
references: BMC Microbiol. 2008 Jan 28;8:20
references: 18226246
references: Appl Environ Microbiol. 2002 Oct;68(10):4710-6
references: 12324311
references: Proc Natl Acad Sci U S A. 2010 Nov 2;107(44):19002-7
references: 20956325
references: J Exp Med. 1988 Apr 1;167(4):1459-71
references: 2833557
references: Appl Environ Microbiol. 2008 Jan;74(1):158-71
references: 18024685
references: Infect Immun. 2006 Feb;74(2):876-86
references: 16428730
references: BMC Microbiol. 2009 Feb 03;9:26
references: 19192306
references: Appl Environ Microbiol. 2001 Jun;67(6):2692-8
references: 11375182
references: Proteomics. 2006 Feb;6(4):1301-18
references: 16470664
references: Microbiol Mol Biol Rev. 2004 Jun;68(2):207-33
references: 15187182
references: Mol Microbiol. 1998 Apr;28(1):81-93
references: 9593298
references: Appl Environ Microbiol. 2010 Nov;76(21):6998-7003
references: 20817809
references: Nat Protoc. 2009;4(1):44-57
references: 19131956
references: Infect Immun. 2006 Feb;74(2):1323-38
references: 16428782
references: J Pathol. 1999 Jun;188(2):220-6
references: 10398168
references: J Bacteriol. 2003 Oct;185(19):5722-34
references: 13129943
references: Int J Med Microbiol. 2011 Apr;301(4):334-40
references: 21273119
references: Proteomics. 2009 Dec;9(24):5425-36
references: 19834908
references: PLoS Pathog. 2010 May 13;6(5):e1000900
references: 20485518
references: Infect Immun. 2000 Feb;68(2):999-1003
references: 10639481
references: Thromb Res. 2010 Apr;125 Suppl 1:S49-51
references: 20149923
references: FEMS Microbiol Lett. 2010 Jul 1;308(1):94-100
references: 20487028
references: Microbiology (Reading). 2010 Sep;156(Pt 9):2660-2669
references: 20558511
references: J Food Prot. 2006 Dec;69(12):3031-6
references: 17186676
references: Microbiol Immunol. 1995;39(7):451-6
references: 8569529
references: Microbiology (Reading). 2004 Nov;150(Pt 11):3843-3855
references: 15528669
references: J Bacteriol. 1996 May;178(9):2479-88
references: 8626312
references: PLoS One. 2011;6(11):e27958
references: 22114730
references: Nucleic Acids Res. 2009 Jan;37(1):1-13
references: 19033363
references: Appl Environ Microbiol. 1990 Jun;56(6):1960-2
references: 16348232
references: Appl Environ Microbiol. 2004 May;70(5):2912-8
references: 15128551
references: J Bacteriol. 1998 Jul;180(14):3650-6
references: 9658010
references: FEMS Microbiol Lett. 2005 Apr 15;245(2):329-36
references: 15837390
references: Microbiology (Reading). 2007 Oct;153(Pt 10):3593-3607
references: 17906156
references: J Bacteriol. 1998 Sep;180(17):4547-54
references: 9721294
references: Science. 1999 May 21;284(5418):1318-22
references: 10334980
references: PLoS Pathog. 2007 May 4;3(5):e62
references: 17480118