title: Heme oxygenase-1 promotes Caco-2 cell proliferation and migration by targeting CTNND1.
abstract: BACKGROUND
  Heme oxygenase-1 (HO-1) can be induced by inflammatory cytokines, oxidation, ischemia, hypoxia, and endotoxins. As a "graft survival protective gene," HO-1 is a hot spot in organ transplantation research. However, the role of HO-1 gene expression in the function of human colon adenocarcinoma cell line (Caco-2) cells has not been reported previously.
  
  
  METHODS
  The role of HO-1 in the proliferation and migration of Caco-2 cells was analyzed using a stable HO-1 expression plasmid. We constructed a recombinant adeno-associated virus plasmid containing the HO-1 gene, heme oxygenase 1 (HMOX1), which was transfected into Caco-2 intestinal cells. We identified a number of target genes by global microarray analysis combined with real-time polymerase chain reaction (PCR) and chromatin immunoprecipitation assay.
  
  
  RESULTS
  Our results showed that significant HO-1 upregulation was demonstrated in the Caco-2 cells after HO-1 transfection. Restoration of HO-1 expression promoted proliferation and invasion in vitro. The CTNND1 gene, a member of the armadillo protein family, was identified as a direct HO-1 target gene.
  
  
  CONCLUSIONS
  Overexpression of HO-1 promotes Caco-2 cell proliferation and migration by targeting the CTNND1 gene.
authors: Department of Gastrointestinal Surgery, Provincial Hospital Affiliated to Shandong University, Jinan, Shandong, China.
authors: Li
authors: L
authors: Zhang
authors: Yu-lin
authors: YL
authors: Liu
authors: Guang-xiang
authors: GX
authors: Chen
authors: Bin
authors: B
authors: Cui
authors: Jin-shen
authors: JS
authors: Wang
authors: Yu-long
authors: YL
authors: Shi
authors: Le-ping
authors: LP
authors: Li
authors: Xiao-bo
authors: XB
authors: Guo
mesh_terms: D018938:Caco-2 Cells; D051177:Catenins / Q000235:genetics*; D002465:Cell Movement; D049109:Cell Proliferation; D015972:Gene Expression Regulation, Neoplastic; D051547:Heme Oxygenase-1 / Q000235:genetics / Q000502:physiology*; D006801:Humans; D060888:Real-Time Polymerase Chain Reaction; D000095002:Delta Catenin
chemical_list: D051177:Catenins; C496541:HMOX1 protein, human; D051547:Heme Oxygenase-1; D000095002:Delta Catenin