title: In vitro micropropagation of Dracaena sanderiana Sander ex Mast: An important indoor ornamental plant.
abstract: A protocol has been developed for in vitro plant regeneration from a nodal explant of Dracaena sanderiana Sander ex Mast. Nodal explant showed high callus induction potentiality on MS medium supplemented with 6.78 μM 2,4-dichlorophenoxyacetic acid (2,4-D) followed by 46.5 μM chlorophenoxy acetic acid (CPA). The highest frequency of shoot regeneration (85%) and number of shoots per explant (5.6) were obtained on medium supplemented with 7.84 μM N(6)-benzylaminopurine (BA). Rooting was high on MS solid compared to liquid medium when added with 7.38 μM indole-3-butyric acid (IBA). Fifty percent of the roots were also directly rooted as microcuttings on soil rite, sand and peat mixture (1:1:1). In vitro and ex vitro raised plantlets were used for acclimatization. More than 90% of the plantlets was successfully acclimatized and established in plastic pots. Ex vitro transferred plantlets were normal without any phenotypic aberrations.
authors: Cellular Differentiation and Molecular Genetics Section, Department of Botany, Hamdard University, New Delhi 110 062, India ; Department of Biotechnology, Jamia Hamdard (Hamdard University), New Delhi 110 062, India.
authors: Junaid
authors: J
authors: Aslam
authors: Abdul
authors: A
authors: Mujib
authors: Maheshwar Prasad
authors: MP
authors: Sharma
keywords: Dracaena sanderiana; In vitro rooting; Indoor ornamental; Plant regeneration; Shoot multiplication
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