title: Adaptive responses by mouse fetus to a maternal HLE diet by downregulating SREBP1: a microarray- and bio-analytic-based study.
abstract: Maternal diet has long been recognized as a significant factor affecting offspring development and health, but the target genes affected by a maternal high-lipid diet are currently unknown. In this study, the gene expression profile of neonatal mouse liver was analyzed using gene chips to identify genes with significant up- or downregulated expression levels due to maternal high-fat diet during gestation. Real-time PCR and Western blotting were used to measure key genes selected using microarray. Serum lipid, glucose, and insulin levels in adult offspring from dams fed with chow or a high-lipid diet were measured using commercial kits. Results indicate that the expression of genes involved in cholesterol and fatty acid synthesis were significantly inhibited, while the expression of genes involved in glycolysis were significantly decreased by maternal high-lipid diet during gestation. SREBP1 might be the key gene regulating genes involved in fatty acid, glucose, and cholesterol metabolism in response to a maternal high-fat diet.
authors: School of Public Health and Capital Medical University, Beijing 100069, China.
authors: Huan-Ling
authors: HL
authors: Yu
authors: Huang-Tai
authors: HT
authors: Miao
authors: Li-Fang
authors: LF
authors: Gao
authors: Li
authors: L
authors: Li
authors: Yuan-Di
authors: YD
authors: Xi
authors: Shao-Ping
authors: SP
authors: Nie
authors: Rong
authors: R
authors: Xiao
mesh_terms: D000222:Adaptation, Physiological / Q000187:drug effects*; D000818:Animals; D002784:Cholesterol / Q000097:blood; D002791:Cholesterol, Dietary / Q000009:adverse effects*; D059305:Diet, High-Fat / Q000009:adverse effects*; D015536:Down-Regulation / Q000187:drug effects*; D005260:Female; D005333:Fetus / Q000378:metabolism / Q000502:physiology*; D006903:Hydroxymethylglutaryl CoA Reductases / Q000235:genetics; D007328:Insulin / Q000097:blood; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D051379:Mice; D009035:Mothers; D020411:Oligonucleotide Array Sequence Analysis; D011247:Pregnancy; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051780:Sterol Regulatory Element Binding Protein 1 / Q000235:genetics*
chemical_list: D002791:Cholesterol, Dietary; D007328:Insulin; D012333:RNA, Messenger; D051780:Sterol Regulatory Element Binding Protein 1; D002784:Cholesterol; D006903:Hydroxymethylglutaryl CoA Reductases
keywords: dietary cholesterol; gestational diet; high-energy high-lipid diet; maternal diet
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