title: Eliminating false positives in a qPCR assay for the detection of the uidA gene in Escherichia coli.
abstract: Due to contaminant Escherichia coli DNA present in recombinant Taq polymerase reagents, it is not possible to reliably detect low levels of E. coli in samples using the quantitative polymerase chain reaction (qPCR) assay. Native Taq polymerase was successfully used in this study to detect five uidA gene copies (5 fg of genomic DNA) of the uidA gene.
authors: Centre for Research on Environmental Microbiology, University of Ottawa, Ottawa, ON K1H 8M5, Canada.
authors: Richard
authors: R
authors: Kibbee
authors: Natalie
authors: N
authors: Linklater
authors: Banu
authors: B
authors: Ormeci
mesh_terms: D001431:Bacteriological Techniques; D004269:DNA, Bacterial / Q000235:genetics*; D003851:Deoxyribonucleases / Q000235:genetics; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D005189:False Positive Reactions; D005798:Genes, Bacterial; D016133:Polymerase Chain Reaction / Q000379:methods*; D019914:Taq Polymerase; D014871:Water Microbiology
chemical_list: D004269:DNA, Bacterial; D019914:Taq Polymerase; D003851:Deoxyribonucleases