title: Xiao-Ai-Ping, a TCM Injection, Enhances the Antigrowth Effects of Cisplatin on Lewis Lung Cancer Cells through Promoting the Infiltration and Function of CD8(+) T Lymphocytes.
abstract: Objectives. To investigate how Xiao-Ai-Ping injection, a traditional Chinese medicine and an ancillary drug in tumor treatment, enhances the antitumor effects of cisplatin on Lewis lung cancer (LLC) cells. Methods. LLC-bearing mice were daily intraperitoneally injected with various doses of cisplatin, Xiao-Ai-Ping, or cisplatin plus Xiao-Ai-Ping, respectively. Body weight and tumor volumes were measured every three days. Results. Combination of Xiao-Ai-Ping and cisplatin yielded significantly better antigrowth and proapoptotic effects on LLC xenografts than sole drug treatment did. In addition, we found that Xiao-Ai-Ping triggered the infiltration of CD8(+) T cells, a group of cytotoxic T cells, to LLC xenografts. Furthermore, the mRNA levels of interferon- γ (ifn- γ ), perforin-1 (prf-1), and granzyme B (gzmb) in CD8(+) T cells were significantly increased after combination treatment of Xiao-Ai-Ping and cisplatin. In vitro studies showed that Xiao-Ai-Ping markedly upregulated the mRNA levels of ifn- γ , prf-1, and gzmb in CD8(+) T cells in a concentration-dependent manner, suggesting that Xiao-Ai-Ping augments the function of CD8(+) T cells. Conclusions. Xiao-Ai-Ping promotes the infiltration and function of CD8(+) T cells and thus enhances the antigrowth effects of cisplatin on LLC xenografts, which provides new evidence for the combination of Xiao-Ai-Ping and cisplatin in clinic in China.
authors: State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, 22 Hankou Road, Nanjing 210093, China.
authors: Wanshuai
authors: W
authors: Li
authors: Yang
authors: Y
authors: Yang
authors: Zijun
authors: Z
authors: Ouyang
authors: Qi
authors: Q
authors: Zhang
authors: Lu
authors: L
authors: Wang
authors: Feifei
authors: F
authors: Tao
authors: Yongqian
authors: Y
authors: Shu
authors: Yanhong
authors: Y
authors: Gu
authors: Qiang
authors: Q
authors: Xu
authors: Yang
authors: Y
authors: Sun
references: Methods Mol Biol. 2012;864:515-34
references: 22367910
references: PLoS Pathog. 2012;8(3):e1002557
references: 22396645
references: N Engl J Med. 2012 Jun 28;366(26):2455-65
references: 22658128
references: Cancer Lett. 2013 Feb 1;329(1):9-16
references: 22960596
references: Oncol Lett. 2013 Mar;5(3):917-922
references: 23426522
references: Cancer J. 2012 Mar-Apr;18(2):160-75
references: 22453018
references: Cancer Res. 2013 Apr 15;73(8):2493-504
references: 23418322
references: Cancer Immunol Immunother. 2013 Feb;62(2):203-16
references: 23389507
references: Biochem Pharmacol. 2012 Jul 15;84(2):172-81
references: 22525724
references: Breast Cancer. 2013 Oct;20(4):302-9
references: 23456736
references: Anticancer Agents Med Chem. 2012 Jan;12(1):49-75
references: 21707502
references: J Thorac Oncol. 2010 Sep;5(9):1410-5
references: 20683208
references: EMBO Rep. 2009 Mar;10(3):194-200
references: 19229284
references: J Immunol. 2006 Sep 1;177(5):3004-11
references: 16920936
references: Exp Ther Med. 2013 Jan;5(1):138-142
references: 23251255
references: J Med Chem. 2012 Aug 23;55(16):7003-9
references: 22712563
references: N Engl J Med. 2012 Jun 28;366(26):2443-54
references: 22658127
references: PLoS Pathog. 2012;8(4):e1002645
references: 22532799
references: J Immunol. 2010 Jun 1;184(11):5954-8
references: 20427765
references: Gene Ther. 2003 Aug;10(16):1311-20
references: 12883527
references: Neoplasia. 2013 Mar;15(3):335-47
references: 23479511
references: J Nucl Med. 2013 Jan;54(1):104-10
references: 23283564
references: Neoplasia. 2013 Jan;15(1):85-94
references: 23359264
references: Steroids. 2010 Feb;75(2):176-83
references: 19925814
references: Phytochemistry. 1996 Mar;41(5):1385-8
references: 8729461
references: Methods Mol Biol. 2012;918:117-26
references: 22893289
references: Trends Mol Med. 2013 Feb;19(2):108-16
references: 23306374
references: Pharm Res. 2010 Jun;27(6):950-61
references: 20238150
references: Nature. 2011 Dec 21;480(7378):480-9
references: 22193102
references: Biochem Biophys Res Commun. 2013 Jan 25;430(4):1283-8
references: 23261451
references: Cancer Immunol Immunother. 2012 Oct;61(10):1671-82
references: 22382361
references: PLoS One. 2013;8(1):e47434
references: 23326300
references: Immunol Lett. 2013 Mar;151(1-2):71-5
references: 23439006
references: Oncol Lett. 2013 Jan;5(1):321-327
references: 23255942
references: Front Oncol. 2013 Feb 01;3:14
references: 23378947