title: The effective mechanism of the polysaccharides from Panax ginseng on chronic fatigue syndrome.
abstract: Ginseng acidic polysaccharide WGPA isolated from the root of Panax ginseng C. A. Meyer was fractionated into WGPA-A and WGPA-N by anion-exchange chromatography. The antifatigue activity of ginseng acidic polysaccharide WGPA has been reported in our previous research. This present study was designed to identify its active component and elucidate the mechanism for preventing chronic fatigue syndrome (CFS). WGPA, WGPA-A and WGPA-N were orally administered to mice once daily for 15 days. The effects of these compounds on physiological biomarkers of oxidative stress and on the morphology of the mitochondria in striated skeletal muscle were assessed. The results of forced swimming test-induced indicated that WGPA and WGPA-A could lengthen the swimming time, while WGPA-N could not. In addition, malondialdehyde and lactate dehydrogenase levels in serum were enhanced; while those of superoxide dismutase and glutathione peroxidase were lowered. Interestingly, the structural degeneration of mitochondria were all ameliorated. These findings suggested that WGPA-A is the active component of WGPA, it might have potential therapeutic effects for CFS and the oxidative stress might be involved in the pathogenesis. Our results also provided essential data for a better understanding of the antifatigue effects of P. ginseng extracts.
authors: Jilin Province Key Laboratory on Chemistry and Biology of Changbai Mountain Natural Drugs School of Life Sciences, Northeast Normal University, Changchun, 130024, People's Republic of China.
authors: Jia
authors: J
authors: Wang
authors: Chengxin
authors: C
authors: Sun
authors: Yan
authors: Y
authors: Zheng
authors: Hongling
authors: H
authors: Pan
authors: Yifa
authors: Y
authors: Zhou
authors: Yuying
authors: Y
authors: Fan
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use; D015415:Biomarkers / Q000097:blood; D004195:Disease Models, Animal; D005221:Fatigue / Q000097:blood / Q000188:drug therapy / Q000378:metabolism; D015673:Fatigue Syndrome, Chronic / Q000097:blood / Q000188:drug therapy* / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000097:blood; D007770:L-Lactate Dehydrogenase / Q000097:blood; D008297:Male; D008315:Malondialdehyde / Q000097:blood / Q000378:metabolism; D051379:Mice; D008928:Mitochondria / Q000187:drug effects / Q000648:ultrastructure; D054792:Muscle, Striated / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D018384:Oxidative Stress / Q000187:drug effects; D005894:Panax / Q000737:chemistry*; D008517:Phytotherapy; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use*; D018517:Plant Roots / Q000737:chemistry; D011134:Polysaccharides / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use*; D013482:Superoxide Dismutase / Q000097:blood; D013550:Swimming
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D010936:Plant Extracts; D011134:Polysaccharides; D008315:Malondialdehyde; D007770:L-Lactate Dehydrogenase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase