title: Reagents for developmental regulation of Hedgehog signaling.
abstract: We have examined a number of reagents for their ability to modulate activity of the Hh signaling pathway during embryonic development of Xenopus. In particular we have focused on regulation of events occurring during tailbud stages and later. Two inducible protein reagents based on the Gli1 and Gli3 transcription factors were generated and the activity of these proteins was compared to the Hh signaling pathway inhibitor, cyclopamine, and the activators, Smoothened agonist (SAG) and purmorphamine (PMA). Effectiveness of reagents was assayed using both molecular biological techniques and biological readouts. We found that the small molecule modulators of the Hh pathway were highly specific and effective and produced results generally superior to the more conventional protein reagents for examination of later stage developmental processes.
authors: Department of Cellular and Molecular Medicine, University of Arizona College of Medicine, Tucson, AZ, United States.
authors: Cristy
authors: C
authors: Lewis
authors: Department of Cellular and Molecular Medicine, University of Arizona College of Medicine, Tucson, AZ, United States. Electronic address: pkrieg@email.arizona.edu.
authors: Paul A
authors: PA
authors: Krieg
mesh_terms: D000818:Animals; D003514:Cyclohexylamines / Q000494:pharmacology; D046149:Embryo Culture Techniques; D047108:Embryonic Development / Q000187:drug effects*; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects*; D053823:Hedgehog Proteins / Q000502:physiology*; D009025:Morpholines / Q000494:pharmacology; D011687:Purines / Q000494:pharmacology; D015398:Signal Transduction / Q000187:drug effects; D013876:Thiophenes / Q000494:pharmacology; D014704:Veratrum Alkaloids / Q000494:pharmacology; D014981:Xenopus
chemical_list: D003514:Cyclohexylamines; D053823:Hedgehog Proteins; D009025:Morpholines; D011687:Purines; C468918:SAG compound; D013876:Thiophenes; D014704:Veratrum Alkaloids; C470893:purmorphamine; C000541:cyclopamine
keywords: Cyclopamine; Dorsal aorta; Gli1; Gli3; Hedgehog; Purmorphamine (PMA); Smoothened agonist (SAG)