title: Molecular characterization of herpes simplex virus 2 strains by analysis of microsatellite polymorphism.
abstract: The complete 154-kbp linear double-stranded genomic DNA sequence of herpes simplex virus 2 (HSV-2), consisting of two extended regions of unique sequences bounded by a pair of inverted repeat elements, was published in 1998 and since then has been widely employed in a wide range of studies. Throughout the HSV-2 genome are scattered 150 microsatellites (also referred to as short tandem repeats) of 1- to 6-nucleotide motifs, mainly distributed in noncoding regions. Microsatellites are considered reliable markers for genetic mapping to differentiate herpesvirus strains, as shown for cytomegalovirus and HSV-1. The aim of this work was to characterize 12 polymorphic microsatellites within the HSV-2 genome by use of 3 multiplex PCR assays in combination with length polymorphism analysis for the rapid genetic differentiation of 56 HSV-2 clinical isolates and 2 HSV-2 laboratory strains (gHSV-2 and MS). This new system was applied to a specific new HSV-2 variant recently identified in HIV-1-infected patients originating from West Africa. Our results confirm that microsatellite polymorphism analysis is an accurate tool for studying the epidemiology of HSV-2 infections.
authors: UPMC University of Paris, Paris, France.
authors: Sonia
authors: S
authors: Burrel
authors: Zaïna
authors: Z
authors: Ait-Arkoub
authors: Delphine
authors: D
authors: Voujon
authors: Claire
authors: C
authors: Deback
authors: Emiliana P
authors: EP
authors: Abrao
authors: Henri
authors: H
authors: Agut
authors: David
authors: D
authors: Boutolleau
mesh_terms: D000354:Africa, Western; D014644:Genetic Variation; D006558:Herpes Genitalis / Q000821:virology; D018258:Herpesvirus 2, Human / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D018895:Microsatellite Repeats; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length; D014773:Virology / Q000379:methods*
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