title: Mutational analysis of quinolone resistance protein QnrB1.
abstract: Alanine substitutions and selected deletions have been used to localize amino acids in QnrB essential for its protective activity. Essential amino acids are found at positions i and i(-2) in the pentapeptide repeat module and in the larger of two loops, where deletion of only a single amino acid compromises activity. Deletion of 10 amino acids at the N terminus is tolerated, but removal of 3 amino acids in the C-terminal dimerization unit destroys activity.
authors: Lahey Hospital and Medical Center, Burlington, Massachusetts, USA.
authors: George A
authors: GA
authors: Jacoby
authors: Marian A
authors: MA
authors: Corcoran
authors: Debra M
authors: DM
authors: Mills
authors: Caitlin M
authors: CM
authors: Griffin
authors: David C
authors: DC
authors: Hooper
mesh_terms: D019943:Amino Acid Substitution; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D004252:DNA Mutational Analysis; D024881:Drug Resistance, Bacterial / Q000187:drug effects / Q000235:genetics*; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000235:genetics* / Q000378:metabolism; D016297:Mutagenesis, Site-Directed; D009154:Mutation; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D055503:Protein Multimerization; D017434:Protein Structure, Tertiary; D015363:Quinolones / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D029968:Escherichia coli Proteins; D020033:Protein Isoforms; C485384:Qnr protein, E coli; D015363:Quinolones
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