title: Lipoxin a4 preconditioning and postconditioning protect myocardial ischemia/reperfusion injury in rats.
abstract: This study aims to investigate the pre- and postconditioning effects of lipoxin A4 (LXA4) on myocardial damage caused by ischemia/reperfusion (I/R) injury. Seventy-two rats were divided into 6 groups: sham groups (C1 and C2), I/R groups (I/R1 and I/R2), and I/R plus LXA4 preconditioning and postconditioning groups (LX1 and LX2). The serum levels of IL-1 β , IL-6, IL-8, IL-10, TNF- α , and cardiac troponin I (cTnI) were measured. The content and the activity of Na(+)-K(+)-ATPase as well as the superoxide dismutase (SOD), and malondialdehyde (MDA) levels were determined. Along with the examination of myocardium ultrastructure and ventricular arrhythmia scores (VAS), connexin 43 (Cx43) expression were also detected. Lower levels of IL-1 β , IL-6, IL-8, TNF- α , cTnI, MDA content, and VAS and higher levels of IL-10, SOD activity, Na(+)-K(+)-ATPase content and activity, and Cx43 expression appeared in LX groups than I/R groups. Besides, H&E staining, TEM examination as well as analysis of gene, and protein confirmed that LXA4 preconditioning was more effective than postconditioning in preventing arrhythmogenesis via the upregulation of Cx43. That is, LXA4 postconditioning had better protective effect on Na(+)-K(+)-ATPase and myocardial ultrastructure.
authors: Department of Cardiovascular and Thoracic Surgery, The 2nd Affiliated Hospital & Yuying Children's Hospital of Wenzhou Medical University, 109 College Western Road, Wenzhou 325027, China. zhaoqf1862@163.com
authors: Qifeng
authors: Q
authors: Zhao
authors: Lan
authors: L
authors: Shao
authors: Xingti
authors: X
authors: Hu
authors: Guowei
authors: G
authors: Wu
authors: Jie
authors: J
authors: Du
authors: Jie
authors: J
authors: Xia
authors: Huixian
authors: H
authors: Qiu
mesh_terms: D000818:Animals; D001145:Arrhythmias, Cardiac / Q000378:metabolism / Q000517:prevention & control; D018031:Connexin 43 / Q000378:metabolism; D016207:Cytokines / Q000378:metabolism; D004562:Electrocardiography; D005786:Gene Expression Regulation; D007249:Inflammation; D057775:Ischemic Postconditioning; D019194:Ischemic Preconditioning; D044045:Lipoxins / Q000494:pharmacology*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D046529:Microscopy, Electron, Transmission; D015428:Myocardial Reperfusion Injury / Q000473:pathology* / Q000517:prevention & control; D009206:Myocardium / Q000473:pathology / Q000648:ultrastructure; D018384:Oxidative Stress; D051381:Rats; D017207:Rats, Sprague-Dawley; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism; D019210:Troponin I / Q000378:metabolism
chemical_list: D018031:Connexin 43; D016207:Cytokines; D044045:Lipoxins; D019210:Troponin I; C040527:lipoxin A4; D008315:Malondialdehyde; D013482:Superoxide Dismutase; D000254:Sodium-Potassium-Exchanging ATPase
references: Cardiovasc Res. 1988 Jul;22(7):447-55
references: 3252968
references: Am J Physiol. 1995 Jul;269(1 Pt 2):H297-307
references: 7631860
references: Cardiovasc Res. 2011 Jan 1;89(1):51-9
references: 20724308
references: Eur J Pharmacol. 2005 Dec 28;528(1-3):124-31
references: 16324693
references: Pharmacology. 2009;84(5):294-9
references: 19816089
references: Am J Respir Crit Care Med. 2009 Aug 15;180(4):311-9
references: 19483113
references: J Immunol. 2009 Mar 15;182(6):3819-26
references: 19265161
references: Basic Res Cardiol. 2011 Nov;106(6):925-52
references: 21892746
references: J Appl Physiol (1985). 2006 Oct;101(4):1252-61
references: 16794020
references: Biochem Pharmacol. 2013 Jun 1;85(11):1634-43
references: 23567997
references: Front Immunol. 2012 Aug 28;3:270
references: 22973272
references: FASEB J. 2008 Apr;22(4):1204-12
references: 17984180
references: Microbes Infect. 2008 Jan;10(1):21-8
references: 18068391
references: Am J Physiol Heart Circ Physiol. 2011 Jun;300(6):H2280-7
references: 21441313
references: Acta Physiol (Oxf). 2011 Apr;201(4):435-44
references: 21070611
references: Cardiovasc Res. 2007 Jun 1;74(3):396-405
references: 17362896
references: Biochim Biophys Acta. 2012 Aug;1818(8):2020-9
references: 21839722
references: Proc Natl Acad Sci U S A. 1984 Sep;81(17):5335-9
references: 6089195
references: J Am Coll Cardiol. 2003 Feb 19;41(4):681-6
references: 12598083
references: Inflammation. 2013 Apr;36(2):468-75
references: 23114480
references: Cardiovasc Res. 2008 Oct 1;80(1):9-19
references: 18519446
references: Cardiovasc Res. 2008 Mar 1;77(4):757-65
references: 18056766
references: J Immunol. 2007 Dec 15;179(12):8533-43
references: 18056401
references: Biochim Biophys Acta. 2009 Jul;1787(7):794-801
references: 19328770
references: J Mol Neurosci. 2012 Sep;48(1):185-200
references: 22661361
references: Am J Physiol Heart Circ Physiol. 2013 Jan 1;304(1):H131-41
references: 23103492
references: Antioxid Redox Signal. 2009 Feb;11(2):251-66
references: 18831678
references: J Surg Res. 2012 Jun 1;175(1):176-84
references: 21944479
references: Cardiovasc Res. 2006 Nov 1;72(2):241-9
references: 16914125
references: Eur J Pharmacol. 2013 Jul 15;712(1-3):16-21
references: 23665492
references: Pflugers Arch. 2009 Apr;457(6):1207-26
references: 18853183
references: Prog Biophys Mol Biol. 2007 May-Jun;94(1-2):120-43
references: 17470375
references: Free Radic Biol Med. 2012 Dec 15;53(12):2263-8
references: 23085513
references: Int J Cardiol. 1997 Jan 31;58(2):95-117
references: 9049675
references: Am J Physiol Heart Circ Physiol. 2004 Jan;286(1):H214-21
references: 14500130
references: Inflamm Res. 2007 Aug;56(8):324-33
references: 17687517
references: Am J Physiol Heart Circ Physiol. 2013 Jan 1;304(1):H94-103
references: 23086991
references: Cardiovasc Res. 2004 Feb 15;61(3):386-401
references: 14962471
references: Nat Rev Immunol. 2008 May;8(5):349-61
references: 18437155
references: J Extra Corpor Technol. 2004 Dec;36(4):391-411
references: 15679285
references: Kardiol Pol. 2010 Jan;68(1):91-6
references: 20131195
references: J Clin Invest. 2010 Jan;120(1):266-79
references: 20038810
references: Ann Thorac Surg. 1999 Nov;68(5):1905-12
references: 10585102
references: Cell Tissue Res. 2013 Apr;352(1):21-31
references: 22940728
references: Semin Immunopathol. 2013 Mar;35(2):151-61
references: 23370700
references: Acta Pharmacol Sin. 2013 Apr;34(4):501-6
references: 23524571