title: Divergent astrovirus associated with neurologic disease in cattle.
abstract: Using viral metagenomics of brain tissue from a young adult crossbreed steer with acute onset of neurologic disease, we sequenced the complete genome of a novel astrovirus (BoAstV-NeuroS1) that was phylogenetically related to an ovine astrovirus. In a retrospective analysis of 32 cases of bovine encephalitides of unknown etiology, 3 other infected animals were detected by using PCR and in situ hybridization for viral RNA. Viral RNA was restricted to the nervous system and detected in the cytoplasm of affected neurons within the spinal cord, brainstem, and cerebellum. Microscopically, the lesions were of widespread neuronal necrosis, microgliosis, and perivascular cuffing preferentially distributed in gray matter and most severe in the cerebellum and brainstem, with increasing intensity caudally down the spinal cord. These results suggest that infection with BoAstV-NeuroS1 is a potential cause of neurologic disease in cattle.
authors: Blood Systems Research Institute, San Francisco, California, USA.
authors: Linlin
authors: L
authors: Li
authors: Santiago
authors: S
authors: Diab
authors: Sabrina
authors: S
authors: McGraw
authors: Bradd
authors: B
authors: Barr
authors: Ryan
authors: R
authors: Traslavina
authors: Robert
authors: R
authors: Higgins
authors: Tom
authors: T
authors: Talbot
authors: Pat
authors: P
authors: Blanchard
authors: Guillermo
authors: G
authors: Rimoldi
authors: Elizabeth
authors: E
authors: Fahsbender
authors: Brady
authors: B
authors: Page
authors: Tung Gia
authors: TG
authors: Phan
authors: Chunlin
authors: C
authors: Wang
authors: Xutao
authors: X
authors: Deng
authors: Patricia
authors: P
authors: Pesavento
authors: Eric
authors: E
authors: Delwart
mesh_terms: D000818:Animals; D019200:Astroviridae / Q000145:classification / Q000235:genetics* / Q000648:ultrastructure; D019350:Astroviridae Infections / Q000150:complications*; D001921:Brain / Q000473:pathology / Q000821:virology; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology* / Q000209:etiology*; D005814:Genes, Viral; D001257:Mamastrovirus / Q000145:classification / Q000235:genetics / Q000648:ultrastructure; D056186:Metagenomics; D008969:Molecular Sequence Data; D009422:Nervous System Diseases / Q000662:veterinary*; D016366:Open Reading Frames; D010802:Phylogeny; D012189:Retrospective Studies; D013116:Spinal Cord / Q000473:pathology / Q000821:virology
keywords: Astrovirus; bovine; brain; cattle; in situ hybridization; neurologic disease; next-generation sequencing; viruses
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