title: The effect of T-2 toxin on percentages of CD4+, CD8+, CD4+ CD8+ and CD21+ lymphocytes, and mRNA expression levels of selected cytokines in porcine ileal Peyer's patches.
abstract: The immune system is one of the main toxicity targets of the T-2 toxin. In view of scant research data demonstrating the effect of T-2 on cellular and humoral responses in gut-associated lymphoid tissue (GALT), this study set out to investigate the effects of chronic exposure to low doses of the T-2 toxin (200 microg T-2 toxin kg(-1) feed) on percentages of CD4+ and CD8+ T lymphocytes, CD4+/CD8+ double-positive T lymphocytes, CD21+ B cells, and IL-2, IFN-gamma, IL-4 and IL-10 mRNA expression levels in porcine ileal Peyer's patches. The investigated material comprised ileum sections sampled from piglets (aged 8-10 weeks, body weight of 15-18 kg) on days 14, 28 and 42 of the experiment. After 42 days of exposure to T-2, a significant drop in the quantity of the IL-10 product was observed (R = 0.94; S.E. 0.49-0.79; p < 0.001). A gradual decrease in the amount of IL-4 and IFN-gamma cytokine transcripts was found throughout the experiment, but the reported trend was not significant. On experimental days 14 and 42, a significant increase in the percentage of CD8+ T lymphocytes was observed in comparison with the control (p = 0.04 and p = 0.05, respectively), whereas on day 28, a significant decrease in the percentage of the above subpopulation was noted (p = 0.00). The percentage of CD21+ B cells in the experimental group decreased steadily in comparison with the control, and the observed drop was significant on days 28 and 42 (p = 0.06 and p = 0.00, respectively). On days 14 and 28, the percentages of CD4+ and CD8+ T lymphocytes were lower in the experimental animals than in the control group, and the drop reported on day 28 was statistically significant (p = 0.03).
authors: Department of Veterinary Prevention and Feed Hygiene, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego 13, 10-718 Olsztyn, Poland. kazimierz.obremski@uwm.edu.pl
authors: K
authors: K
authors: Obremski
authors: P
authors: P
authors: Podlasz
authors: M
authors: M
authors: Zmigrodzka
authors: A
authors: A
authors: Winnicka
authors: M
authors: M
authors: Woźny
authors: P
authors: P
authors: Brzuzan
authors: E
authors: E
authors: Jakimiuk
authors: P
authors: P
authors: Wojtacha
authors: M
authors: M
authors: Gajecka
authors: Ł
authors: Ł
authors: Zielonka
authors: M
authors: M
authors: Gajecki
mesh_terms: D000821:Animal Feed / Q000032:analysis; D000818:Animals; D015703:Antigens, CD / Q000235:genetics / Q000378:metabolism; D016207:Cytokines / Q000235:genetics / Q000378:metabolism*; D004032:Diet / Q000662:veterinary; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D010581:Peyer's Patches / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D013552:Swine / Q000502:physiology*; D013605:T-2 Toxin / Q000633:toxicity*; D013601:T-Lymphocytes / Q000145:classification / Q000378:metabolism / Q000502:physiology*
chemical_list: D015703:Antigens, CD; D016207:Cytokines; D012333:RNA, Messenger; D013605:T-2 Toxin