title: Genetic tracing via DNGR-1 expression history defines dendritic cells as a hematopoietic lineage.
abstract: Mononuclear phagocytes are classified as macrophages or dendritic cells (DCs) based on cell morphology, phenotype, or select functional properties. However, these attributes are not absolute and often overlap, leading to difficulties in cell-type identification. To circumvent this issue, we describe a mouse model to define DCs based on their ontogenetic descendence from a committed precursor. We show that precursors of mouse conventional DCs, but not other leukocytes, are marked by expression of DNGR-1. Genetic tracing of DNGR-1 expression history specifically marks cells traditionally ascribed to the DC lineage, and this restriction is maintained after inflammation. Notably, in some tissues, cells previously thought to be monocytes/macrophages are in fact descendants from DC precursors. These studies provide an in vivo model for fate mapping of DCs, distinguishing them from other leukocyte lineages, and thus help to unravel the functional complexity of the mononuclear phagocyte system.
authors: Immunobiology Laboratory, Cancer Research UK, London Research Institute, Lincoln's Inn Fields Laboratories, 44 Lincoln's Inn Fields, London WC2A 3LY, UK.
authors: Barbara U
authors: BU
authors: Schraml
authors: Janneke
authors: J
authors: van Blijswijk
authors: Santiago
authors: S
authors: Zelenay
authors: Paul G
authors: PG
authors: Whitney
authors: Andrew
authors: A
authors: Filby
authors: Sophie E
authors: SE
authors: Acton
authors: Neil C
authors: NC
authors: Rogers
authors: Natalia
authors: N
authors: Moncaut
authors: Jaime J
authors: JJ
authors: Carvajal
authors: Caetano
authors: C
authors: Reis e Sousa
mesh_terms: D000818:Animals; D019070:Cell Lineage; D003713:Dendritic Cells / Q000166:cytology* / Q000378:metabolism; D006410:Hematopoiesis; D007249:Inflammation / Q000473:pathology; D007668:Kidney / Q000166:cytology; D037181:Lectins, C-Type / Q000235:genetics / Q000378:metabolism*; D054503:Lymphoid Progenitor Cells / Q000378:metabolism; D008264:Macrophages / Q000166:cytology; D051379:Mice; D008810:Mice, Inbred C57BL; D010586:Phagocytes / Q000166:cytology; D017452:Receptors, IgG / Q000378:metabolism; D011971:Receptors, Immunologic / Q000235:genetics / Q000378:metabolism*
chemical_list: C529914:Clec9a protein, mouse; D037181:Lectins, C-Type; D017452:Receptors, IgG; D011971:Receptors, Immunologic