title: Putting molecules in their place.
abstract: Each class of microscope is limited to imaging specific aspects of cell structure and/or molecular organization. However, imaging the specimen by complementary microscopes and correlating the data can overcome this limitation. Whilst not a new approach, the field of correlative imaging is currently benefitting from the emergence of new microscope techniques. Here we describe the correlation of cryogenic fluorescence tomography (CFT) with soft X-ray tomography (SXT). This amalgamation of techniques integrates 3D molecular localization data (CFT) with a high-resolution, 3D cell reconstruction of the cell (SXT). Cells are imaged in both modalities in a near-native, cryopreserved state. Here we describe the current state of the art in correlative CFT-SXT, and discuss the future outlook for this method.
authors: Department of Anatomy, University of California San Francisco, San Francisco, California.
authors: Bertrand P
authors: BP
authors: Cinquin
authors: Myan
authors: M
authors: Do
authors: Gerry
authors: G
authors: McDermott
authors: Alison D
authors: AD
authors: Walters
authors: Markko
authors: M
authors: Myllys
authors: Elizabeth A
authors: EA
authors: Smith
authors: Orna
authors: O
authors: Cohen-Fix
authors: Mark A
authors: MA
authors: Le Gros
authors: Carolyn A
authors: CA
authors: Larabell
mesh_terms: D006801:Humans; D007091:Image Processing, Computer-Assisted; D021621:Imaging, Three-Dimensional; D008856:Microscopy, Fluorescence / Q000379:methods* / Q000639:trends; D013223:Statistics as Topic; D014056:Tomography, X-Ray / Q000379:methods* / Q000639:trends; D015003:Yeasts / Q000648:ultrastructure*
keywords: CORRELATED IMAGING; FLUORESCENCE; MICROSCOPY; SOFT X-RAY; TOMOGRAPHY
references: Curr Opin Biotechnol. 2009 Feb;20(1):83-9
references: 19345086
references: Trends Cell Biol. 2009 Nov;19(11):587-95
references: 19818625
references: Science. 1999 Mar 12;283(5408):1670-6
references: 10073924
references: J Struct Biol. 2012 Feb;177(2):193-201
references: 22210307
references: J Struct Biol. 2011 Dec;176(3):268-78
references: 21907806
references: Nat Methods. 2010 Dec;7(12):985-7
references: 21076419
references: J Microsc. 2009 Jul;235(1):1-8
references: 19566622
references: Curr Opin Struct Biol. 2010 Oct;20(5):623-31
references: 20869868
references: Methods Cell Biol. 2012;111:259-81
references: 22857933
references: Bioessays. 2012 Apr;34(4):320-7
references: 22290620
references: Curr Opin Struct Biol. 2005 Oct;15(5):593-600
references: 16153818
references: Q Rev Biophys. 1995 Feb;28(1):33-130
references: 7676009
references: Nat Methods. 2005 Oct;2(10):743-9
references: 16179920
references: Nat Methods. 2008 Jun;5(6):545-51
references: 18454154
references: PLoS One. 2012;7(12):e53293
references: 23300909
references: Proc Natl Acad Sci U S A. 2009 Nov 17;106(46):19375-80
references: 19880740
references: Science. 1977 Jun 17;196(4296):1339-40
references: 867033
references: Scanning. 2005 Jan-Feb;27(1):30-4
references: 15712755
references: J Struct Biol. 2010 Nov;172(2):159
references: 20851188
references: J Cell Biol. 2011 Jan 10;192(1):111-9
references: 21200030
references: Curr Opin Struct Biol. 2011 Oct;21(5):686-93
references: 21782417
references: Traffic. 2009 Feb;10(2):131-6
references: 19054388
references: Proc Natl Acad Sci U S A. 2012 Apr 17;109(16):6136-41
references: 22474357
references: J Am Chem Soc. 2013 Feb 20;135(7):2387-402
references: 23317378
references: Methods Mol Biol. 2013;950:457-81
references: 23086890
references: Annu Rev Phys Chem. 2012;63:225-39
references: 22242730
references: J Struct Biol. 2008 Mar;161(3):372-83
references: 18023206
references: Yeast. 2011 Mar;28(3):227-36
references: 21360734
references: Curr Opin Microbiol. 2005 Jun;8(3):316-22
references: 15939356
references: Opt Express. 2012 Jul 30;20(16):18362-9
references: 23038387
references: J Struct Biol. 2012 Feb;177(2):267-72
references: 22119891
references: Ultramicroscopy. 2000 Aug;84(3-4):185-97
references: 10945329
references: Science. 2006 Apr 14;312(5771):217-24
references: 16614209
references: Ultramicroscopy. 1977 Aug;2(4):337-49
references: 919076
references: J Struct Biol. 2007 Nov;160(2):135-45
references: 17884579
references: Trends Cell Biol. 1999 Feb;9(2):81-5
references: 10087625
references: Biophys J. 1995 Apr;68(4):1416-22
references: 7787027
references: Methods Cell Biol. 2012;111:139-55
references: 22857927
references: Methods Cell Biol. 1999;58:1-18
references: 9891371
references: Trends Biochem Sci. 2009 Feb;34(2):60-70
references: 19101147
references: Micron. 2012 Apr;43(5):565-82
references: 22244153
references: Nat Methods. 2008 Nov;5(11):973-80
references: 18974735
references: Opt Express. 2009 Sep 28;17(20):17669-77
references: 19907552
references: Cell. 2012 Nov 9;151(4):724-737
references: 23141535
references: Methods Cell Biol. 2007;79:575-91
references: 17327175