title: Oxidative stress and antioxidant enzymes activities in the African catfish, Clarias gariepinus, experimentally challenged with Escherichia coli and Vibrio fischeri.
abstract: The impacts of bacterial infection on cultivated fish species, African catfish, were investigated using oxidative stress biomarkers [lipid peroxidation (LPO) and protein carbonylation] and the activities of important antioxidant/detoxifying enzymes [catalase and glutathione S-transferase (GST)]. Fish were inoculated via oral gavage with one of the following treatments: 1 × 10(5) CFU/ml of Escherichia coli (EC1), 2 × 10(5) CFU/ml of E. coli (EC2), 1 × 10(5) CFU/ml of Vibrio fischeri (V1), 2 × 10(5) CFU/ml of V. fischeri (V2), gavaged with distilled water and not gavaged. Fish were maintained in the laboratory for 7 days after the bacterial inoculation, and the levels of LPO, protein carbonylation, GST, and catalase activities were determined in the muscle, gills, and liver of fish. Fish inoculated with bacteria (either E. coli or V. fischeri) had a significant higher levels of tissue LPO, protein carbonylation, and GST activities in a tissue-specific pattern (liver > muscle > gills). This appears to be related with the levels of bacterial inoculation, with effects more pronounced in fish inoculated with either EC2 or V2. The catalase activity did not differ significantly between the inoculated and fish that were not inoculated. The results of this study indicate that bacterial inoculation could result in oxidative stress in fish, and liver has a higher rate of oxidative stress per mg tissue compared to the gills and the muscle.
authors: Department of Biological Sciences, College of Science, Engineering and Technology, Osun State University, P.M.B. 4494, Osogbo, Osun State, Nigeria, joseph.adeyemi@gmail.com.
authors: Joseph A
authors: JA
authors: Adeyemi
mesh_terms: D059707:Aliivibrio Infections / Q000378:metabolism / Q000662:veterinary; D048908:Aliivibrio fischeri; D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D002397:Catfishes / Q000378:metabolism* / Q000382:microbiology*; D004927:Escherichia coli Infections / Q000378:metabolism / Q000662:veterinary; D005393:Fish Diseases / Q000378:metabolism; D029941:Fish Proteins / Q000378:metabolism; D005880:Gills / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D015227:Lipid Peroxidation; D008099:Liver / Q000378:metabolism; D009132:Muscles / Q000378:metabolism; D018384:Oxidative Stress; D050050:Protein Carbonylation
chemical_list: D000975:Antioxidants; D029941:Fish Proteins; D002374:Catalase; D005982:Glutathione Transferase
references: Environ Toxicol Pharmacol. 2009 Sep;28(2):302-10
references: 21784020
references: Aquat Toxicol. 2008 Jan 31;86(2):197-204
references: 18063143
references: Comp Biochem Physiol C Toxicol Pharmacol. 2009 Nov;150(4):521-7
references: 19651240
references: Methods Enzymol. 1994;233:357-63
references: 8015470
references: Environ Toxicol Chem. 2012 Jul;31(7):1573-8
references: 22511216
references: Microbiol Mol Biol Rev. 2004 Sep;68(3):403-31, table of contents
references: 15353563
references: Methods Enzymol. 2000;322:413-21
references: 10914037
references: J Biol Chem. 1974 Nov 25;249(22):7130-9
references: 4436300
references: Trends Microbiol. 1999 Oct;7(10):414-20
references: 10498950
references: J Biol Chem. 1992 Jan 25;267(3):1840-5
references: 1730722
references: Anal Biochem. 1976 May 7;72:248-54
references: 942051