title: Programmable interactions of functionalized single bioparticles in a dielectrophoresis-based microarray chip.
abstract: Manipulating single biological objects is a major unmet challenge of biomedicine. Herein, we describe a lab-on-a-chip platform based on dielectrophoresis (DEP). The DEParray is a prototypal version consisting of 320 × 320 arrayed electrodes generating >10,000 spherical DEP cages. It allows the capture and software-guided movement to predetermined spatial coordinates of single biological objects. With the DEParray we demonstrate (a) forced interaction between a single, preselected target cell and a programmable number of either microspheres or natural killer (NK) cells, (b) on-chip immunophenotypic discrimination of individual cells based on differential rosetting with microspheres functionalized with monoclonal antibodies to an inhibitory NK cell ligand (HLA-G), (c) on-chip, real-time (few minutes) assessment of immune lysis by either visual inspection or semiautomated, time-lapse reading of a fluorescent dye released from NK cell-sensitive targets, and (d) manipulation and immunophenotyping with limiting amounts (about 500) cells. To our knowledge, this is the first report describing a DEP-based lab-on-a-chip platform for the quick, arrayed, software-guided binding of individually moved biological objects, the targeting of single cells with microspheres, and the real-time characterization of immunophenotypes. The DEParray candidates as a discovery tool for novel cell:cell interactions with no prior (immuno)phenotypic knowledge.
authors: Silicon Biosystems, Bologna, Italy.
authors: Melanie
authors: M
authors: Abonnenc
authors: Nicolò
authors: N
authors: Manaresi
authors: Monica
authors: M
authors: Borgatti
authors: Gianni
authors: G
authors: Medoro
authors: Enrica
authors: E
authors: Fabbri
authors: Aldo
authors: A
authors: Romani
authors: Luigi
authors: L
authors: Altomare
authors: Marco
authors: M
authors: Tartagni
authors: Roberta
authors: R
authors: Rizzo
authors: Olavio
authors: O
authors: Baricordi
authors: Elisa
authors: E
authors: Tremante
authors: Elisa
authors: E
authors: Lo Monaco
authors: Patrizio
authors: P
authors: Giacomini
authors: Roberto
authors: R
authors: Guerrieri
authors: Roberto
authors: R
authors: Gambari
mesh_terms: D046209:Electrophoresis, Microchip / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D020014:K562 Cells; D007694:Killer Cells, Natural / Q000378:metabolism*; D008863:Microspheres; D011485:Protein Binding / Q000502:physiology