title: Bioorthogonal approach to identify unsuspected drug targets in live cells.
abstract: A proteomics method to pull down secondary drug targets from live cells is described. The drug of interest is modified with trans-cyclooctene (TCO) and incubated with live cells. Upon cell lysis, the modified drug bound to the protein is pulled down using magnetic beads decorated with a cleavable tetrazine-modified linker. Samples are then run on an SDS-PAGE gel and isolated bands are submitted for mass spectrometry analysis to identify drug targets.
authors: Center for Systems Biology, Massachusetts General Hospital, 185 Cambridge Street, Boston, MA 02114 (USA).
authors: Katherine S
authors: KS
authors: Yang
authors: Ghyslain
authors: G
authors: Budin
authors: Carlos
authors: C
authors: Tassa
authors: Olivier
authors: O
authors: Kister
authors: Ralph
authors: R
authors: Weissleder
mesh_terms: D006801:Humans; D058990:Molecular Targeted Therapy / Q000379:methods*; D040901:Proteomics / Q000379:methods*
keywords: bioorthogonal reaction; cleavable linkers; drug targets; proteins; proteomics
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