title: Isolation and culture of porcine embryonic stem cells.
abstract: Despite their agricultural and biomedical importance, embryonic stem cells (ESCs) are yet to be isolated for the pig or the domestic ungulates in general. This suggests that methods which have been used successfully in mice may not be applicable to these. In this chapter we describe a new method for the isolation of porcine ESCs. This method differs from those described previously in that it produces homogeneous outgrowths from undifferentiated inner cell mass cells when embryos are plated onto inactivated mouse embryonic feeder layers.
authors: Reproductive Biotechnology Group, School of Paediatrics and Reproductive Health and Centre for Stem Cell Research, Robinson Institute, The University of Adelaide, Adelaide, SA, Australia.
authors: Ivan
authors: I
authors: Vassiliev
authors: Mark B
authors: MB
authors: Nottle
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000166:cytology*; D002454:Cell Differentiation; D002460:Cell Line; D004622:Embryo, Mammalian; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000378:metabolism; D061252:Feeder Cells; D005347:Fibroblasts / Q000166:cytology; D006801:Humans; D051379:Mice; D039904:Pluripotent Stem Cells / Q000166:cytology*; D013552:Swine