title: Composite-induced toxicity in human gingival and pulp fibroblast cells.
abstract: OBJECTIVE
  The most important requirement for a material to be used in medical applications is its biocompatibility. Dental composite materials come into direct contact with oral tissues, especially gingival and pulpal cells. This study was performed to evaluate possible DNA damage in cells of human origin exposed to dental composites in vitro using a cytogenetic assay.
  
  
  METHODS
  Two composite resins (Vertise Flow, Kalore) were tested on human gingival and pulp fibroblasts using the acridine orange/ethidium bromide viability staining and alkaline comet assay. Cultures were treated with polymerized composites in two different concentrations (20 mg/ml, 40 mg/ml) for 14 days. Chi-square and Kruskall-Wallis non-parametric test were used for the statistical analysis (p < 0.05).
  
  
  RESULTS
  Significant cytotoxicity was observed for 40 mg/ml of Vertise Flow in both cultures, while Kalore (40 mg/ml) showed cytotoxic effect only on human pulp fibroblasts. A significant level of DNA damage was detected for both materials and concentrations, in both cell cultures.
  
  
  CONCLUSIONS
  If the two cell cultures are compared, the pulp cells were more sensitive to the cyto/genotoxic effects of dental composites. Based on the results, one can conclude that the use of tested materials may cause cellular damage in gingival and pulp fibroblasts in vitro.
authors: Department of Endodontics and Restorative Dental Medicine, Study of Dental Medicine, School of Medicine, University of Split , Split , Croatia.
authors: Antonija
authors: A
authors: Tadin
authors: Danijela
authors: D
authors: Marovic
authors: Nada
authors: N
authors: Galic
authors: Ivan
authors: I
authors: Kovacic
authors: Davor
authors: D
authors: Zeljezic
mesh_terms: D000293:Adolescent; D000328:Adult; D020552:Comet Assay; D003764:Dental Materials / Q000633:toxicity*; D003782:Dental Pulp / Q000166:cytology / Q000187:drug effects*; D005347:Fibroblasts / Q000187:drug effects; D005881:Gingiva / Q000166:cytology / Q000187:drug effects*; D006801:Humans; D055815:Young Adult
chemical_list: D003764:Dental Materials