title: Do mesenchymal stem cells modulate the milieu of reconstructed bladder wall?
abstract: To evaluate the mesenchymal stem cells (MSCs) influence on cytokines and matrix metalloproteinases (MMPs) expression in rat bladder wall regeneration. MSCs cultures from the bone marrow were established. Acellular matrices from the bladder submucosa were prepared. Bladders were reconstructed using cell-seeded (n = 5) and unseeded (n = 5) grafts. MSCs were injected into the bladder wall (n = 5), bladders were incised and MSCs were injected into the circulation (n = 5) or were left intact (n = 5). Animals were killed after 3 months. Bladder histology and immunohistochemical staining of IL-2, IL-4, IL-6, IL-10, TNF-α, TGF-β1, IFN-γ, MMP-2, and MMP-9 were done. Bladders reconstructed with cell-seeded grafts mimicked native tissue, while unseeded grafts revealed shrinkage and morphological irregularities. There were no morphological changes in bladders of other groups. Different pattern of cytokine and MMP expression was observed. Increased expression of anti-inflammatory cytokines and MMPs in bladder promotes detrusor regeneration.
authors: Department of Tissue Engineering, Ludwik Rydygier Medical College in Bydgoszcz, Nicolaus Copernicus University in Torun, Karlowicza 24, 85-092, Bydgoszcz, Poland, marta.pokrywczynska@interia.pl.
authors: Marta
authors: M
authors: Pokrywczynska
authors: Arkadiusz
authors: A
authors: Jundzill
authors: Magdalena
authors: M
authors: Bodnar
authors: Jan
authors: J
authors: Adamowicz
authors: Jakub
authors: J
authors: Tworkiewicz
authors: Lukasz
authors: L
authors: Szylberg
authors: Robert
authors: R
authors: Debski
authors: Andrzej
authors: A
authors: Marszalek
authors: Tomasz
authors: T
authors: Drewa
mesh_terms: D000818:Animals; D001853:Bone Marrow / Q000473:pathology; D002478:Cells, Cultured; D016207:Cytokines / Q000276:immunology*; D005786:Gene Expression Regulation; D008297:Male; D020780:Matrix Metalloproteinase 9 / Q000235:genetics / Q000378:metabolism*; D059630:Mesenchymal Stem Cells / Q000502:physiology*; D032389:Myocytes, Smooth Muscle / Q000502:physiology*; D051381:Rats; D011913:Rats, Inbred BB; D012038:Regeneration; D033581:Stem Cell Transplantation; D001743:Urinary Bladder / Q000293:injuries / Q000502:physiology*
chemical_list: D016207:Cytokines; D020780:Matrix Metalloproteinase 9
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