title: Gold nanoparticles paper as a SERS bio-diagnostic platform.
abstract: Bioactive papers are usually challenged by four major limitations: sensitivity, selectivity, simplicity and strength (4S). Gold nanoparticles (AuNPs) treated paper has previously been demonstrated as a Surface Enhanced Raman Scattering (SERS) active substrate, capable of addressing the 4S issues. In this study, AuNPs on paper substrate were functionalized by a series of biomolecules to develop a generic SERS platform for antibody-antigen detection. The functionalization steps were performed by taking advantage of the high affinity association between Streptomyces avidinii-derived protein, streptavidin, and biotin. Streptavidin was firstly bound onto the AuNPs treated paper using biotinylated-thiol. Subsequently, desired biotinylated-antibody was bound onto the streptavidin. SERS spectra of each functionalization step were obtained to ensure specific adsorption of the bio-molecules. The binding interaction of the antibody with its specific antigen was detected using SERS. Shifts of Raman band associated with α-helix and β-sheet structures indicated structural modification of the antibody upon interaction with its antigen. Predominant tryptophan and tyrosine residue bands were also detected, confirming the presence of antigen. Reproducible spectral features were quantified as AuNP papers were subjected to different concentrations of antigen; the spectra intensity increased as a function of the antigen concentration. The retention of AuNPs on paper remained constant after all the consecutive washing and functionalization steps. The feasibility of AuNPs paper as a low-cost and generic SERS platform for bio-diagnostic applications was demonstrated.
authors: BioPRIA, Australian Pulp and Paper Institute (APPI), Department of Chemical Engineering, Monash University, Clayton, VIC 3800, Australia.
authors: Ying Hui
authors: YH
authors: Ngo
authors: Whui Lyn
authors: WL
authors: Then
authors: Wei
authors: W
authors: Shen
authors: Gil
authors: G
authors: Garnier
mesh_terms: D000327:Adsorption; D000937:Antigen-Antibody Reactions; D001426:Bacterial Proteins / Q000737:chemistry*; D001710:Biotin / Q000737:chemistry*; D006046:Gold / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D010209:Paper; D010316:Particle Size; D013059:Spectrum Analysis, Raman; D019809:Streptavidin / Q000737:chemistry*; D013302:Streptomyces / Q000737:chemistry; D013499:Surface Properties
chemical_list: D001426:Bacterial Proteins; D001710:Biotin; D006046:Gold; D019809:Streptavidin
keywords: Antibody; Antigen; Biotin; Gold nanoparticles (AuNPs); Paper; Paper bioassay; Streptavidin; Surface Enhanced Raman Scattering (SERS)