title: Different cell responses induced by exposure to maghemite nanoparticles.
abstract: Recent advances in nanotechnology have permitted the development of a wide repertoire of inorganic magnetic nanoparticles (NPs) with extensive promise for biomedical applications. Despite this remarkable potential, many questions still arise concerning the biocompatible nature of NPs when in contact with biological systems. Herein, we have investigated how controlled changes in the physicochemical properties of iron oxide NPs at their surface (i.e., surface charge and hydrodynamic size) affect, first, their interaction with cell media components and, subsequently, cell responses to NP exposure. For that purpose, we have prepared iron oxide NPs with three different coatings (i.e., dimercaptosuccinic acid - DMSA, (3-aminopropyl)triethoxysilane - APS and dextran) and explored the response of two different cell types, murine L929 fibroblasts and human Saos-2 osteoblasts, to their exposure. Interestingly, different cell responses were found depending on the NP concentration, surface charge and cell type. In this sense, neutral NPs, as those coated with dextran, induced negligible cell damage, as their cellular internalization was significantly reduced. In contrast, surface-charged NPs (i.e., those coated with DMSA and APS) caused significant cellular changes in viability, morphology and cell cycle under certain culture conditions, as a result of a more active cellular internalization. These results also revealed a particular cellular ability to detect and remember the original physicochemical properties of the NPs, despite the formation of a protein corona when incubated in culture media. Overall, conclusions from these studies are of crucial interest for future biomedical applications of iron oxide NPs.
authors: Instituto de Ciencia de Materiales de Madrid (ICMM), Consejo Superior de Investigaciones Científicas (CSIC), Calle Sor Juana Inés de la Cruz 3, 28049-Madrid, Spain. conchi.serrano@icmm.csic.es.
authors: Yurena
authors: Y
authors: Luengo
authors: Stefania
authors: S
authors: Nardecchia
authors: María Puerto
authors: MP
authors: Morales
authors: M Concepción
authors: MC
authors: Serrano
mesh_terms: D000818:Animals; D059447:Cell Cycle Checkpoints / Q000187:drug effects; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D003911:Dextrans / Q000737:chemistry; D005290:Ferric Compounds / Q000737:chemistry*; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000633:toxicity; D051379:Mice; D018613:Microscopy, Confocal; D010316:Particle Size; D011437:Propylamines / Q000737:chemistry; D012821:Silanes / Q000737:chemistry; D004113:Succimer / Q000737:chemistry; D013499:Surface Properties
chemical_list: D003911:Dextrans; D005290:Ferric Compounds; D011437:Propylamines; D012821:Silanes; C088294:3-aminopropyltrimethoxysilane; C000499:ferric oxide; D004113:Succimer