title: A bZIP protein, VIP1, interacts with Arabidopsis heterotrimeric G protein β subunit, AGB1.
abstract: Heterotrimeric G proteins (Gα, Gβ, Gγ) are signaling molecules conserved among eukaryotic species. The G proteins transmit signals via protein-protein interactions. By a yeast two-hybrid screen, we identified a bZIP protein, VIP1, as an Arabidopsis thaliana Gβ (AGB1)-interacting partner. The interaction between AGB1 and VIP1 was confirmed by an in vitro GST pull-down assay and a bimolecular fluorescence complementation (BiFC) assay. VIP1 was previously reported to be a regulator of osmosensory signaling. Interestingly, the BiFC pattern between AGB1 and VIP1 was speckled when cells were incubated in a hypotonic solution, but not when cells were incubated in a mannitol-containing hypertonic solution, suggesting that the subcellular localization of the AGB1-VIP1 complex is regulated by extracellular osmolarity and/or turgor pressure.
authors: Asian Natural Environmental Science Center (ANESC), The University of Tokyo, 1-1-1 Midori-cho, Nishitokyo-shi, Tokyo, 188-0002, Japan.
authors: Daisuke
authors: D
authors: Tsugama
authors: Shenkui
authors: S
authors: Liu
authors: Tetsuo
authors: T
authors: Takano
mesh_terms: D017360:Arabidopsis / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000378:metabolism*; D050976:Basic-Leucine Zipper Transcription Factors / Q000378:metabolism*; D044387:GTP-Binding Protein beta Subunits / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant / Q000502:physiology; D011485:Protein Binding / Q000502:physiology; D015398:Signal Transduction / Q000502:physiology
chemical_list: D029681:Arabidopsis Proteins; D050976:Basic-Leucine Zipper Transcription Factors; D044387:GTP-Binding Protein beta Subunits
keywords: ABA; AC; Arabidopsis thaliana; BiFC; G protein-coupled receptor; GAL4 DNA-binding domain; GAL4 activation domain; GAL4AD; GAL4BD; GPCR; Heterotrimeric G protein β subunit; NLS; Osmosensory signaling; PLC; Protein–protein interactions; RbcL; Rubisco large subunit; Transcription factor; Y2H; abscisic acid; adenylate cyclase; bimolecular fluorescence complementation; nuclear localization signal; phospholipase C; yeast two-hybrid