title: Molecular cloning, characterization and mRNA expression of a chitin synthase 2 gene from the oriental fruit fly, Bactrocera dorsalis (Diptera: Tephritidae).
abstract: Chitin synthase (CHS), a potential target for eco-friendly insecticides, plays an essential role in chitin formation in insects. In this study, a full-length cDNA encoding chitin synthase 2 (BdCHS2) was cloned and characterized in the oriental fruit fly, Bactrocera dorsalis. The BdCHS2 cDNA had 4417 nucleotides, containing an open reading frame of 4122 nucleotides, which encoded 1373 amino acid residues with a predicted molecular weight of 158.5 kDa. Phylogenetic analysis with other insect CHSs suggested that BdCHS2 belongs to insect CHS2. The BdCHS2 transcript was predominately found in midgut but was detected at low levels in fat body, Malpighian tubules, integument, and trachea. Moreover, BdCHS2 was expressed in all developmental stages, and highly expressed in the feeding stages. There was a positive relationship between BdCHS2 expression and total chitin content during development. Furthermore, both the gene expression and chitin content in midgut decreased when the insect was fed for 24 h, then starved for 24 h, while they increased dramatically and rapidly under the condition of starvation for 24 h then feeding for 24 h. These results suggest that BdCHS2 may play an important role in regulating chitin content of the midgut, and subsequently affect the growth and development of B. dorsalis.
authors: Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing 400716, China. chenli420625@gmail.com
authors: Li
authors: L
authors: Chen
authors: Wen-Jia
authors: WJ
authors: Yang
authors: Lin
authors: L
authors: Cong
authors: Kang-Kang
authors: KK
authors: Xu
authors: Jin-Jun
authors: JJ
authors: Wang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002686:Chitin / Q000378:metabolism; D002687:Chitin Synthase / Q000096:biosynthesis / Q000235:genetics*; D004435:Eating; D004790:Enzyme Induction; D041981:Gastrointestinal Tract / Q000201:enzymology; D015870:Gene Expression; D019476:Insect Proteins / Q000096:biosynthesis / Q000235:genetics*; D007814:Larva / Q000201:enzymology / Q000235:genetics; D008969:Molecular Sequence Data; D009928:Organ Specificity; D010802:Phylogeny; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D033621:Tephritidae / Q000201:enzymology / Q000235:genetics*
chemical_list: D019476:Insect Proteins; D012333:RNA, Messenger; D002686:Chitin; D002687:Chitin Synthase; C400821:chitin synthase 2
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