title: Induction of cotton ovule culture fibre branching by co-expression of cotton BTL, cotton SIM, and Arabidopsis STI genes.
abstract: The highly elongated single-celled cotton fibre consists of lint and fuzz, similar to the Arabidopsis trichome. Endoreduplication is an important determinant in Arabidopsis trichome initiation and morphogenesis. Fibre development is also controlled by functional homologues of Arabidopsis trichome patterning genes, although fibre cells do not have a branched shape like trichomes. The identification and characterization of the homologues of 10 key Arabidopsis trichome branching genes in Gossypium arboreum are reported here. Nuclear ploidy of fibres was determined, and gene function in cotton callus and fibre cells was investigated. The results revealed that the nuclear DNA content was constant in fuzz, whereas a limited and reversible change occurred in lint after initiation. Gossypeum arboreum branchless trichomes (GaBLT) was not transcribed in fibres. The homologue of STICHEL (STI), which is essential for trichome branching, was a pseudogene in Gossypium. Targeted expression of GaBLT, Arabidopsis STI, and the cytokinesis-repressing GaSIAMESE in G. hirsutum fibre cells cultured in vitro resulted in branching. The findings suggest that the distinctive developmental mechanism of cotton fibres does not depend on endoreduplication. This important component may be a relic function that can be activated in fibre cells.
authors: State Key Laboratory of Cotton Biology, Institute of Cotton Research of Chinese Academy of Agricultural Sciences, Anyang 455000, Henan Province, China.
authors: Gaskin
authors: G
authors: Wang
authors: Hongjie
authors: H
authors: Feng
authors: Junling
authors: J
authors: Sun
authors: Xiongming
authors: X
authors: Du
mesh_terms: D017360:Arabidopsis / Q000235:genetics*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D002467:Cell Nucleus / Q000235:genetics; D003001:Cloning, Molecular; D032561:Cotton Fiber; D048749:Cytokinesis / Q000235:genetics; D018744:DNA, Plant / Q000378:metabolism; D062951:Endoreduplication / Q000235:genetics; D004843:Epistasis, Genetic; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant / Q000235:genetics; D005966:Glucuronidase / Q000378:metabolism; D003368:Gossypium / Q000166:cytology / Q000235:genetics*; D008938:Mitosis / Q000235:genetics; D056771:Ovule / Q000235:genetics* / Q000254:growth & development*; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D030821:Plants, Genetically Modified; D011544:Pseudogenes / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D046509:Tissue Culture Techniques; D064201:Trichomes / Q000254:growth & development
chemical_list: D029681:Arabidopsis Proteins; D018744:DNA, Plant; D010940:Plant Proteins; C474076:STICHEL protein, Arabidopsis; D005966:Glucuronidase
keywords: Branch; Gossypium; endoreduplication; fuzz; lint; nuclear DNA content.
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