title: Cortisol and testosterone in hair as biological markers of systolic heart failure.
abstract: BACKGROUND
  Congestive heart failure (CHF) is associated with increased stress and alterations in metabolism, favoring catabolism over anabolism. Hormonal profiles of patients with heart failure have been assessed using serum and saliva as matrices, which are only point measurements and do not provide long-term information. Scalp hair is a novel matrix that allows for measurement of hormones over a period of several months.
  
  
  METHODS
  We aimed to evaluate whether levels of cortisol and testosterone and their ratio (C/T) in hair correlate with severity of heart failure. We conducted a prospective study in ambulatory male patients with a left ventricular ejection fraction (LVEF)≤40%. Hormone levels were measured using immunoassays in the proximal 2 cm of hair (representing approximately two months of systemic hormone exposure). Primary endpoints included the correlation of hair cortisol, testosterone, and C/T levels with the New York Heart Association (NYHA) class, LVEF, exercise capacity and NT-proBNP.
  
  
  RESULTS
  The 44 CHF patients had a median hair level (range) of cortisol of 207 (117.7-1277.3)ng/g. Hair cortisol levels correlated positively with NYHA class (r=0.48, p=0.001) and negatively with treadmill stress test performance, (r=-0.37, p<0.05). The hair testosterone was 5.17 (2.39-24.64)ng/g and the C/T ratio was 39.89 (12.98-173.73). No associations were found between hair testosterone and C/T ratio and heart failure severity; however, the C/T ratio was higher in patients who required a CHF-related hospitalization than in patients who did not require this in the year following the inclusion in the study.
  
  
  CONCLUSIONS
  Hair cortisol levels correlate with heart failure severity as assessed by the NYHA class and exercise capacity, while hair testosterone and C/T levels do not correlate with heart failure severity.
authors: Division of Cardiology, Meir Medical Center, and Sackler Faculty of Medicine, Tel-Aviv University, Israel.
authors: David
authors: D
authors: Pereg
authors: Justin
authors: J
authors: Chan
authors: Evan
authors: E
authors: Russell
authors: Tatiana
authors: T
authors: Berlin
authors: Morris
authors: M
authors: Mosseri
authors: Jamie A
authors: JA
authors: Seabrook
authors: Gideon
authors: G
authors: Koren
authors: Stan
authors: S
authors: Van Uum
mesh_terms: D000368:Aged; D015415:Biomarkers / Q000032:analysis*; D004452:Echocardiography; D023381:Endpoint Determination; D005080:Exercise Test; D005260:Female; D006197:Hair / Q000737:chemistry*; D054143:Heart Failure, Systolic / Q000378:metabolism* / Q000503:physiopathology; D006801:Humans; D006854:Hydrocortisone / Q000032:analysis*; D007118:Immunoassay; D008297:Male; D011379:Prognosis; D013318:Stroke Volume; D013739:Testosterone / Q000032:analysis*; D016277:Ventricular Function, Left / Q000502:physiology
chemical_list: D015415:Biomarkers; D013739:Testosterone; D006854:Hydrocortisone
keywords: Cortisol; Hair analysis; Heart failure; Prognosis; Testosterone