title: Sensitive and rapid detection of blaNDM-1 in clinical samples by isothermal cross-priming amplification.
abstract: A highly sensitive, specific diagnostic assay for detection of bla(NDM-1) based on cross priming amplification (CPA) was developed. The sensitivity ranged from 2.5 to 25 copies per reaction for different clinical samples. The highly and sensitive detection of bla(NDM-1) in clinical samples highlighted the potential clinical applications of CPA.
authors: Institute of Disease Control and Prevention, Academy of Military Medical Sciences, Beijing 100071, PR China.
authors: Yuehua
authors: Y
authors: Ke
authors: Yufei
authors: Y
authors: Wang
authors: Zhoujia
authors: Z
authors: Wang
authors: Xinying
authors: X
authors: Du
authors: Liuyu
authors: L
authors: Huang
authors: Zeliang
authors: Z
authors: Chen
mesh_terms: D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification*; D005243:Feces / Q000382:microbiology; D006801:Humans; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA; D013183:Sputum / Q000382:microbiology; D001618:beta-Lactamases / Q000302:isolation & purification*
chemical_list: D004269:DNA, Bacterial; D001618:beta-Lactamases; C552864:beta-lactamase NDM-1
keywords: Cross priming amplification; Isothermal amplification; bla(NDM-1)