title: Cytogenetic abnormality in exfoliated cells of buccal mucosa in head and neck cancer patients in the Tunisian population: impact of different exposure sources.
abstract: Chromosome/DNA instability could be one of the primary causes of malignant cell transformation. The objective of the present study was to evaluate the spontaneous genetic damages in exfoliated cells of buccal mucosa of head and neck cancer (HNC) by counting micronucleus (MN) and binucleated (BN) cells frequencies. MN and BN frequencies were significantly increased in HNC patients compared with controls (5.53 ± 3.09/1000 cells, 5.63 ± 2.99/1000 cells versus 2.36 ± 2.11/1000 cells, 3.09 ± 1.82/1000 cells, P < 0.001). Regarding the gender and the age, the frequencies of the MN and BN were significantly higher than those of controls (P < 0.01). The evaluation of the MN and BN frequencies revealed a significant increase (P < 0.001) in the cases in relation to the control group after controlling the risk factors (tobacco smoking and chewing and occupational exposure) of HNC. Moreover, MN and BN frequencies were significantly increased in smokers and chewers compared with nonsmokers and nonchewers among patients (P < 0.05). MN frequency was significantly (P = 0.014) different between patients occupationally exposed (6.99 ± 3.40/1000 cells) and nonexposed (4.70 ± 2.48/1000 cells) among HNC group. The logistic regression model illustrated that HNC was significantly associated with frequencies of MN (OR = 8.63, P < 0.0001) and BN (OR = 5.62, P = 0.001). Our results suggest that increased chromosome/DNA instabilities may be associated with HNC.
authors: Unit of Marine and Environmental Toxicology, UR 09-03, Sfax University, IPEIS, BP 1172, 3018 Sfax, Tunisia. rimkhlifi@yahoo.fr
authors: Rim
authors: R
authors: Khlifi
authors: Fatma
authors: F
authors: Trabelsi-Ksibi
authors: Amine
authors: A
authors: Chakroun
authors: Ahmed
authors: A
authors: Rebai
authors: Amel
authors: A
authors: Hamza-Chaffai
mesh_terms: D000328:Adult; D000368:Aged; D002467:Cell Nucleus / Q000235:genetics / Q000473:pathology*; D002471:Cell Transformation, Neoplastic / Q000235:genetics / Q000473:pathology; D002869:Chromosome Aberrations; D005260:Female; D042822:Genomic Instability; D006258:Head and Neck Neoplasms / Q000235:genetics / Q000473:pathology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009061:Mouth Mucosa / Q000473:pathology*; D016273:Occupational Exposure; D012907:Smoking / Q000009:adverse effects; D014416:Tunisia
references: Carcinogenesis. 2005 May;26(5):991-9
references: 15705599
references: Mutagenesis. 1997 Jul;12(4):285-7
references: 9237775
references: Toxicol Ind Health. 2012 Feb;28(1):74-82
references: 21636684
references: Mutat Res. 2004 Oct 10;563(2):131-8
references: 15364279
references: Mutat Res. 2009 Apr 30;675(1-2):1-4
references: 19386239
references: Mutat Res. 1999 Feb 19;439(2):259-66
references: 10023078
references: Environ Sci Pollut Res Int. 2013 Nov;20(11):8282-94
references: 23625117
references: Mutagenesis. 2001 Nov;16(6):539-45
references: 11682646
references: Clin Exp Rheumatol. 2002 Mar-Apr;20(2):208-12
references: 12051400
references: Mutat Res. 2011 Apr 3;721(2):178-83
references: 21281739
references: Biomaterials. 2005 May;26(14):1713-9
references: 15576145
references: HNO. 2000 Jun;48(6):444-50
references: 10929225
references: Scand J Dent Res. 1983 Apr;91(2):150-2
references: 6222462
references: Mutagenesis. 2006 Mar;21(2):131-7
references: 16513656
references: Cancer Epidemiol Biomarkers Prev. 2006 Jun;15(6):1061-77
references: 16775162
references: Mutagenesis. 2011 Mar;26(2):295-301
references: 21044989
references: Immunol Allergy Clin North Am. 2005 Nov;25(4):709-21
references: 16257634
references: Oral Oncol. 2008 Apr;44(4):354-60
references: 17936677
references: Int J Occup Med Environ Health. 2011 Sep;24(3):308-19
references: 21833695
references: Eur J Cancer B Oral Oncol. 1995 May;31B(3):155-9
references: 7549753
references: Am J Clin Nutr. 2006 Apr;83(4):835-41
references: 16600936
references: Mutat Res. 1997 Jun;386(3):219-28
references: 9219560
references: Mutat Res. 2000 May 8;467(2):105-11
references: 10838197
references: Neoplasma. 2003;50(5):380-2
references: 14628093
references: Mutat Res. 2003 Jun;543(3):251-72
references: 12787816
references: Toxicol Mech Methods. 2010 Jun;20(5):260-6
references: 20450262
references: Cancer Res. 2000 Mar 15;60(6):1619-25
references: 10749131
references: Acta Cytol. 2006 Jul-Aug;50(4):398-402
references: 16901002
references: Mutat Res. 2000 Nov 20;455(1-2):81-95
references: 11113469
references: Environ Mol Mutagen. 2006 Aug;47(7):562-70
references: 16795086
references: Toxicol Lett. 2012 Jul 7;212(1):33-7
references: 22564757
references: Beijing Da Xue Xue Bao Yi Xue Ban. 2011 Aug 18;43(4):600-2
references: 21844976
references: Mutat Res. 2003 Jan 10;534(1-2):65-75
references: 12504755
references: Anal Quant Cytol Histol. 2011 Oct;33(5):271-6
references: 22611754
references: Biochimie. 2006 Nov;88(11):1515-31
references: 16919864
references: Mutagenesis. 2003 Sep;18(5):417-21
references: 12960409
references: Mutat Res. 1998 Sep 11;417(2-3):101-14
references: 9733936
references: Mutat Res. 1999 Jul 16;428(1-2):271-83
references: 10517999
references: Genet Mol Res. 2010 Sep 28;9(3):1921-8
references: 20882488
references: Environ Mol Mutagen. 2013 Jan;54(1):65-71
references: 23055270
references: Oral Oncol. 2007 Feb;43(2):187-92
references: 16860590
references: Mutat Res. 2001 Aug 22;495(1-2):147-56
references: 11448652
references: Mutagenesis. 2006 Mar;21(2):93-103
references: 16567350
references: J Rheumatol. 2004 Jul;31(7):1335-9
references: 15229952
references: Mutat Res. 1992 Feb;271(1):69-77
references: 1371831
references: Mutat Res. 2006 Sep;613(1):1-9
references: 16638643
references: Tohoku J Exp Med. 2010 Feb;220(2):115-20
references: 20139662
references: Mutagenesis. 2000 May;15(3):261-9
references: 10792021
references: J Nucl Med. 2004 Apr;45(4):608-11
references: 15073256
references: Crit Rev Oral Biol Med. 2004 Sep 01;15(5):252-63
references: 15470264
references: Mutagenesis. 2011 Mar;26(2):351-6
references: 21248276
references: Acta Cytol. 1997 Jul-Aug;41(4):1008-14
references: 9250292
references: Mutagenesis. 2011 Jan;26(1):43-9
references: 21164181
references: Eur Arch Otorhinolaryngol. 2005 Jul;262(7):561-6
references: 15619126
references: Mutat Res. 2011 Mar 18;721(1):15-20
references: 21256246
references: Carcinogenesis. 2006 Mar;27(3):517-24
references: 16162645
references: Ecotoxicol Environ Saf. 2013 Jun;92:265-70
references: 23474067
references: Int J Cancer. 1983 Mar 15;31(3):305-8
references: 6826255
references: Int J Dent. 2012;2012:457054
references: 22315605
references: Minerva Stomatol. 2001 Nov-Dec;50(11-12):361-71
references: 11744882
references: Biomarkers. 2002 Mar-Apr;7(2):151-61
references: 12101634
references: Drug Discov Today. 2002 Nov 15;7(22):1128-37
references: 12546856
references: J Dent Res. 2001 Jul;80(7):1615-20
references: 11597020
references: Mutat Res. 2008 Jul-Aug;659(1-2):93-108
references: 18514568
references: J Radiat Res. 2005 Jun;46(2):135-42
references: 15988130
references: Pesqui Odontol Bras. 2002 Jul-Sep;16(3):221-5
references: 12386683
references: Environ Mol Mutagen. 1996;27(3):176-84
references: 8625953
references: Cancer Res. 2004 Sep 15;64(18):6805-13
references: 15375000
references: Environ Mol Mutagen. 2002;40(2):101-9
references: 12203402
references: Occup Med (Lond). 2001 Mar;51(2):107-13
references: 11307685