title: The Arabidopsis metacaspase9 degradome.
abstract: Metacaspases are distant relatives of the metazoan caspases, found in plants, fungi, and protists. However, in contrast with caspases, information about the physiological substrates of metacaspases is still scarce. By means of N-terminal combined fractional diagonal chromatography, the physiological substrates of metacaspase9 (MC9; AT5G04200) were identified in young seedlings of Arabidopsis thaliana on the proteome-wide level, providing additional insight into MC9 cleavage specificity and revealing a previously unknown preference for acidic residues at the substrate prime site position P1'. The functionalities of the identified MC9 substrates hinted at metacaspase functions other than those related to cell death. These results allowed us to resolve the substrate specificity of MC9 in more detail and indicated that the activity of phosphoenolpyruvate carboxykinase 1 (AT4G37870), a key enzyme in gluconeogenesis, is enhanced upon MC9-dependent proteolysis.
authors: Department of Plant Systems Biology, VIB, 9052 Ghent, Belgium.
authors: Liana
authors: L
authors: Tsiatsiani
authors: Evy
authors: E
authors: Timmerman
authors: Pieter-Jan
authors: PJ
authors: De Bock
authors: Dominique
authors: D
authors: Vercammen
authors: Simon
authors: S
authors: Stael
authors: Brigitte
authors: B
authors: van de Cotte
authors: An
authors: A
authors: Staes
authors: Marc
authors: M
authors: Goethals
authors: Tine
authors: T
authors: Beunens
authors: Petra
authors: P
authors: Van Damme
authors: Kris
authors: K
authors: Gevaert
authors: Frank
authors: F
authors: Van Breusegem
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000378:metabolism; D017360:Arabidopsis / Q000201:enzymology* / Q000235:genetics; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D055162:Biocatalysis; D020169:Caspases / Q000235:genetics / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant; D005943:Gluconeogenesis; D008969:Molecular Sequence Data; D050505:Mutant Proteins / Q000378:metabolism; D010455:Peptides / Q000737:chemistry / Q000378:metabolism; D019764:Phosphoenolpyruvate Carboxykinase (ATP) / Q000378:metabolism; D030821:Plants, Genetically Modified; D011499:Protein Processing, Post-Translational; D021381:Protein Transport; D059748:Proteolysis; D020543:Proteome / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism; D015203:Reproducibility of Results; D013347:Subcellular Fractions / Q000201:enzymology; D013379:Substrate Specificity
chemical_list: D000596:Amino Acids; D029681:Arabidopsis Proteins; D050505:Mutant Proteins; D010455:Peptides; D020543:Proteome; D011994:Recombinant Proteins; D020169:Caspases; C516211:metacaspase 9, Arabidopsis; D019764:Phosphoenolpyruvate Carboxykinase (ATP)
references: Cell Death Differ. 2011 Aug;18(8):1289-97
references: 21546909
references: FEBS Lett. 2007 Dec 11;581(29):5635-9
references: 18005666
references: Plant Cell. 2004 Oct;16(10):2705-18
references: 15367715
references: Cell Death Differ. 2012 Aug;19(8):1358-69
references: 22402587
references: Nat Protoc. 2011 Jul 14;6(8):1130-41
references: 21799483
references: Bioinformatics. 2005 Sep 1;21(17):3584-5
references: 16030071
references: J Cell Biol. 2004 Feb 16;164(4):501-7
references: 14970189
references: Plant Cell. 2001 Jul;13(7):1499-510
references: 11449047
references: Plant J. 2008 Oct;56(2):303-315
references: 18573193
references: Trends Biochem Sci. 1999 Feb;24(2):47-53
references: 10098397
references: J Cell Biol. 2005 Aug 1;170(3):391-9
references: 16061692
references: Plant Physiol. 2002 Jan;128(1):165-72
references: 11788762
references: Plant Cell Environ. 2012 Aug;35(8):1440-55
references: 22380487
references: Plant Physiol Biochem. 2011 Jun;49(6):646-53
references: 21398135
references: Proc Natl Acad Sci U S A. 2005 Oct 4;102(40):14463-8
references: 16183741
references: Biol Chem. 2004 Jun;385(6):493-504
references: 15255181
references: Nature. 1970 Aug 15;227(5259):680-5
references: 5432063
references: Mol Microbiol. 2011 Jan;79(1):222-39
references: 21166905
references: Cell Death Differ. 2011 Aug;18(8):1279-88
references: 21597462
references: Biol Chem. 2010 Aug;391(8):983-97
references: 20536382
references: Plant Cell. 2007 Dec;19(12):4111-9
references: 18156219
references: Proteins. 2002 Mar 1;46(4):355-67
references: 11835511
references: J Biol Chem. 2007 Jan 12;282(2):1352-8
references: 17110382
references: Biotechnol J. 2010 Feb;5(2):183-6
references: 20108272
references: Proc Natl Acad Sci U S A. 2012 Feb 7;109(6):1913-8
references: 22308409
references: J Cell Sci. 2006 Mar 15;119(Pt 6):1105-17
references: 16507595
references: Nat Methods. 2005 Oct;2(10):771-7
references: 16179924
references: J Cell Biol. 2007 Nov 5;179(3):375-80
references: 17967946
references: PLoS One. 2008 Aug 13;3(8):e2956
references: 18698411
references: Physiol Plant. 2012 May;145(1):28-40
references: 22008056
references: Biochem Biophys Res Commun. 1971 Oct 15;45(2):278-85
references: 5160716
references: Cell Death Differ. 2012 Dec;19(12):2040-8
references: 22918439
references: Plant Physiol. 2007 May;144(1):479-86
references: 17337522
references: Gene. 2010 Jun 1;457(1-2):25-34
references: 20214955
references: J Biol Chem. 2004 Oct 29;279(44):45329-36
references: 15326173
references: Mol Cell. 2000 Oct;6(4):961-7
references: 11090634
references: J Mol Biol. 2006 Dec 8;364(4):625-36
references: 17028019
references: Mol Microbiol. 2007 Jan;63(2):591-604
references: 17176258
references: Proc Natl Acad Sci U S A. 2010 Jul 27;107(30):13348-53
references: 20624963
references: Nat Biotechnol. 2008 Jun;26(6):685-94
references: 18500335
references: Plant Cell Environ. 2007 Feb;30(2):225-35
references: 17238913
references: J Toxicol Sci. 2009 Oct;34(5):541-5
references: 19797862
references: Cell Death Differ. 2007 Jan;14(1):66-72
references: 17082814
references: Nat Biotechnol. 2003 May;21(5):566-9
references: 12665801
references: Physiol Plant. 2012 May;145(1):1-4
references: 22420761
references: Arch Biochem Biophys. 2003 Jun 15;414(2):204-10
references: 12781772
references: Cell Death Differ. 2008 Jan;15(1):113-22
references: 17901875
references: Plant Cell Environ. 2010 Mar;33(3):418-30
references: 20002332
references: Biochem J. 1993 Feb 15;290 ( Pt 1):205-18
references: 8439290
references: Nat Struct Mol Biol. 2009 Oct;16(10):1101-8
references: 19767749
references: Proc Natl Acad Sci U S A. 2012 May 8;109(19):7469-74
references: 22529389
references: Mol Cell. 2002 Apr;9(4):911-7
references: 11983181
references: Proc Natl Acad Sci U S A. 2007 Nov 13;104(46):18073-8
references: 17984052
references: Cell Death Differ. 2011 Aug;18(8):1247-56
references: 21475301
references: Plant J. 2011 Jun;66(6):969-82
references: 21395887
references: FEBS Lett. 1995 Mar 27;362(1):70-4
references: 7698356
references: Physiol Plant. 2012 May;145(1):5-17
references: 22023699
references: Plant Physiol. 1973 Oct;52(4):357-61
references: 16658562
references: Dev Biol. 1987 Sep;123(1):198-212
references: 2957260
references: New Phytol. 2013 Oct;200(2):498-510
references: 23834670
references: Int J Parasitol. 2007 Feb;37(2):161-72
references: 17107676
references: J Biol Chem. 2011 Nov 18;286(46):39914-25
references: 21949125
references: Annu Rev Biochem. 2011;80:1055-87
references: 21456965
references: Nat Cell Biol. 2009 Nov;11(11):1347-54
references: 19820703
references: Biochem J. 2004 Dec 1;384(Pt 2):201-32
references: 15450003
references: J Exp Bot. 2008;59(3):491-9
references: 18272922
references: Nat Methods. 2009 Nov;6(11):786-7
references: 19876014
references: J Biol Chem. 2011 Mar 25;286(12):10027-40
references: 21209078
references: J Biol Chem. 2005 Apr 15;280(15):14691-9
references: 15691845
references: J Cell Biol. 2007 Apr 9;177(1):7-11
references: 17420288
references: EMBO J. 2012 Apr 4;31(7):1630-43
references: 22367392
references: Mol Microbiol. 2007 Aug;65(4):948-58
references: 17627766
references: Biochim Biophys Acta. 2011 Dec;1813(12):2044-9
references: 21982825
references: Plant Cell Physiol. 2007 Mar;48(3):441-50
references: 17283014
references: Biochem Biophys Res Commun. 1967 Apr 20;27(2):157-62
references: 6035483
references: Biophys J. 1997 Nov;73(5):2782-90
references: 9370472
references: Cell Death Differ. 2011 Aug;18(8):1298-304
references: 21637288
references: PLoS One. 2011;6(8):e23867
references: 21858231
references: Plant Physiol. 2003 Apr;131(4):1834-42
references: 12692343
references: Science. 2010 Dec 3;330(6009):1393-7
references: 21097903
references: Annu Rev Plant Biol. 2008;59:191-223
references: 18257708