title: Gene expression profiles of peripheral blood mononuclear cells  in primary biliary cirrhosis.
abstract: Previous studies on gene expression profiles in primary biliary cirrhosis (PBC) have exclusively focused on liver tissue or intrahepatic cells. Since the pathological process is systemic, other complementary studies in blood cells seemed to be reasonable. In this research, we try to explore differentially expressed genes in peripheral blood mononuclear cells (PBMCs) of PBC patients. Nine PBC patients and 9 healthy controls were recruited as Cohort 1 for a microarray study of screening. Total RNA of PBMCs from each individual was isolated and screened by oligonucleotide microarray (22 K). Then, differentially expressed genes were categorized into signaling pathways. Expression levels of three important genes, tyrosine kinase binding protein (TYROBP), C–C motif chemokine 5 (CCL5) and cathepsin L (CTSL) were confirmed by quantitative real-time polymerase chain reaction (qRT-PCR) in a second Cohort 2 (30 PBC patients and 20 healthy controls). Results show that sixty-five genes differentially expressed in PBC were identified, 20 of which were up-regulated and 45 of which were down-regulated. Twenty-seven signaling pathways were identified. TYROBP and CCL5 were proved to be down-regulated in PBC, and CTSL was proved to be up-regulated (p < 0.05) in PBC, which were all consistent with the screening study. In conclusions, the analysis of gene expression in PBMCs of PBC and the comparison of gene profiles between PBMCs and the liver may provide new clues to the pathogenesis of the disease.
authors: Lei
authors: L
authors: Zhang
authors: Danxu
authors: D
authors: Ma
authors: Xi
authors: X
authors: Li
authors: Chuiwen
authors: C
authors: Deng
authors: Qun
authors: Q
authors: Shi
authors: Xin
authors: X
authors: You
authors: Xiaomei
authors: X
authors: Leng
authors: Mengtao
authors: M
authors: Li
authors: Fulin
authors: F
authors: Tang
authors: Fengchun
authors: F
authors: Zhang
authors: Yongzhe
authors: Y
authors: Li
mesh_terms: D000328:Adult; D000368:Aged; D015331:Cohort Studies; D005260:Female; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000473:pathology*; D008105:Liver Cirrhosis, Biliary / Q000473:pathology*; D008297:Male; D046228:Microarray Analysis; D008875:Middle Aged; D060888:Real-Time Polymerase Chain Reaction; D015398:Signal Transduction; D059467:Transcriptome
references: Gut. 2007 Nov;56(11):1615-24
references: 17641080
references: J Clin Pathol. 2007 Oct;60(10):1102-7
references: 17158635
references: Arthritis Rheum. 2010 Jun;62(6):1602-7
references: 20178127
references: J Rheumatol. 2011 Jan;38(1):87-98
references: 20952467
references: J Gastroenterol. 2008;43(11):866-74
references: 19012040
references: Nucleic Acids Res. 2002 Feb 15;30(4):e15
references: 11842121
references: N Engl J Med. 2005 Sep 22;353(12):1261-73
references: 16177252
references: Semin Immunopathol. 2009 Sep;31(3):283-307
references: 19603170
references: J Clin Immunol. 1999 Nov;19(6):422-7
references: 10634216
references: Hepatology. 2005 May;41(5):1106-12
references: 15830397
references: J Autoimmun. 2007 Dec;29(4):246-9
references: 17888625
references: Gene Ther. 2004 Jul;11(13):1040-7
references: 15164093
references: Hepatology. 2009 Jul;50(1):291-308
references: 19554543
references: Transl Res. 2006 Sep;148(3):103-13
references: 16938647
references: J Clin Immunol. 2008 Sep;28(5):520-7
references: 18584314
references: Nat Rev Rheumatol. 2009 May;5(5):257-65
references: 19412192
references: J Autoimmun. 2007 Dec;29(4):203-5
references: 17884336
references: J Autoimmun. 2007 Dec;29(4):262-71
references: 17870412
references: J Autoimmun. 2006 Jun;26(4):232-40
references: 16730427
references: J Clin Invest. 2005 Oct;115(10):2934-43
references: 16184198
references: Curr Mol Med. 2009 Feb;9(1):45-51
references: 19199941
references: J Autoimmun. 2005 Sep;25(2):133-40
references: 16150573
references: Clin Exp Immunol. 1996 Aug;105(2):254-9
references: 8706330
references: J Autoimmun. 2007 Dec;29(4):257-61
references: 17889507
references: Am J Gastroenterol. 2005 Sep;100(9):2019-30
references: 16128947
references: Gut. 2001 Oct;49(4):565-76
references: 11559656
references: Liver Int. 2008 Aug;28(7):997-1010
references: 18422935
references: Genes Immun. 2009 Jun;10(4):285-96
references: 19404300
references: Arthritis Rheum. 2008 Nov;58(11):3541-9
references: 18975309
references: Scand J Immunol. 2003 Feb;57(2):192-8
references: 12588667
references: Methods. 2001 Dec;25(4):402-8
references: 11846609
references: Proc Natl Acad Sci U S A. 2001 Apr 24;98(9):5116-21
references: 11309499
references: Immunity. 2009 Apr 17;30(4):482-92
references: 19371714
references: Mediators Inflamm. 2004 Dec;13(5-6):357-9
references: 15770052
references: Arthritis Rheum. 2005 May;52(5):1534-44
references: 15880807
references: Hepatology. 2005 Oct;42(4):802-8
references: 16175622