title: Fisetin attenuates hydrogen peroxide-induced cell damage by scavenging reactive oxygen species and activating protective functions of cellular glutathione system.
abstract: Hydrogen peroxide (H2O2) can induce cell damage by generating reactive oxygen species (ROS), resulting in DNA damage and cell death. The aim of this study is to elucidate the protective effects of fisetin (3,7,3',4',-tetrahydroxy flavone) against H2O2-induced cell damage. Fisetin reduced the level of superoxide anion, hydroxyl radical in cell free system, and intracellular ROS generated by H2O2. Moreover, fisetin protected against H2O2-induced membrane lipid peroxidation, cellular DNA damage, and protein carbonylation, which are the primary cellular outcomes of H2O2 treatment. Furthermore, fisetin increased the level of reduced glutathione (GSH) and expression of glutamate-cysteine ligase catalytic subunit, which is decreased by H2O2. Conversely, a GSH inhibitor abolished the cytoprotective effect of fisetin against H2O2-induced cells damage. Taken together, our results suggest that fisetin protects against H2O2-induced cell damage by inhibiting ROS generation, thereby maintaining the protective role of the cellular GSH system.
authors: School of Medicine and Applied Radiological Science Research Institute, Jeju National University, Jeju, 690-756, Republic of Korea.
authors: Kyoung Ah
authors: KA
authors: Kang
authors: Mei Jing
authors: MJ
authors: Piao
authors: Ki Cheon
authors: KC
authors: Kim
authors: Ji Won
authors: JW
authors: Cha
authors: Jian
authors: J
authors: Zheng
authors: Cheng Wen
authors: CW
authors: Yao
authors: Sungwook
authors: S
authors: Chae
authors: Jin Won
authors: JW
authors: Hyun
mesh_terms: D000818:Animals; D002460:Cell Line; D020552:Comet Assay; D003412:Cricetulus; D004249:DNA Damage; D005419:Flavonoids / Q000494:pharmacology*; D044948:Flavonols; D016166:Free Radical Scavengers / Q000494:pharmacology*; D005978:Glutathione / Q000378:metabolism*; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D015227:Lipid Peroxidation; D018384:Oxidative Stress; D020011:Protective Agents / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D005419:Flavonoids; D044948:Flavonols; D016166:Free Radical Scavengers; D020011:Protective Agents; D017382:Reactive Oxygen Species; D006861:Hydrogen Peroxide; D005978:Glutathione; C017875:fisetin
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