title: [Phosphate permease gene as a marker for the specific identification of toxigenic fungus Fusarium cerealis].
abstract: We have developed phosphate permease gene sequence-based PCR detection system of Fusarium cerealis phytopathogenic fungus. Sequencing and analysis revealed that the gene displayed unique polymorphism and could serve to establish phylogenetic relations as well as a marker to design specific primers. The specificity assay has confirmed the absence of cross reactions with DNAs of closely related Fusarium species. The qPCR assay demonstrated the 10 pg detection limit of specific DNA per reaction.
authors: A A
authors: AA
authors: Stakheev
authors: D R
authors: DR
authors: Khaĭrulina
authors: D Iu
authors: DIu
authors: Riazantsev
authors: S K
authors: SK
authors: Zavriev
mesh_terms: D001483:Base Sequence; D005670:Fusarium / Q000201:enzymology / Q000235:genetics* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D028061:Phosphate Transport Proteins / Q000235:genetics* / Q000302:isolation & purification; D010802:Phylogeny; D011110:Polymorphism, Genetic; D013045:Species Specificity
chemical_list: D028061:Phosphate Transport Proteins; C055831:phosphate permease