title: The epigenetically-regulated miR-663 targets H-ras in K-562 cells.
abstract: miR-663 is a tumour suppressor that is potentially regulated by modification of CpG islands. Whether aberrant methylation is one of the reasons for miR-663 down-regulation in some malignant cells and whether miR-663 targets oncogenes warrants further research. In the present study, we report that the CpG islands in the upstream region of pre-miR-663 are aberrantly methylated in the k-562 cell line and in the white blood cells of some chronic myelogenous leukaemia patients, and also that H-ras is one of the genes targeted by miR-663. Over-expression of miR-663 may suppress proliferation of the k-562 cell line in part by enhancing cell apoptosis.
authors: Department of Haematology and BMT Centre, Chinese PLA General Hospital, Beijing, China; Department of Haematology, Chinese PLA Air Force General Hospital, Beijing, China.
authors: Yang
authors: Y
authors: Yang
authors: Li-Li
authors: LL
authors: Wang
authors: Heng-Xiang
authors: HX
authors: Wang
authors: Zi-Kuan
authors: ZK
authors: Guo
authors: Xiao-Fang
authors: XF
authors: Gao
authors: Jian
authors: J
authors: Cen
authors: Yong-Hui
authors: YH
authors: Li
authors: Li-Ping
authors: LP
authors: Dou
authors: Li
authors: L
authors: Yu
mesh_terms: D017209:Apoptosis; D001374:Azacitidine / Q000494:pharmacology; D001483:Base Sequence; D002453:Cell Cycle; D049109:Cell Proliferation; D018899:CpG Islands; D019175:DNA Methylation / Q000187:drug effects; D017931:DNA Primers; D044127:Epigenesis, Genetic; D016147:Genes, Tumor Suppressor; D011905:Genes, ras; D006801:Humans; D020014:K562 Cells; D035683:MicroRNAs / Q000235:genetics / Q000502:physiology*; D016133:Polymerase Chain Reaction
chemical_list: D017931:DNA Primers; C552243:MIRN663 microRNA, human; D035683:MicroRNAs; D001374:Azacitidine
keywords: DNA methylation; H-ras; MiR-663; epigenetics; gene expression