title: Structural and biochemical studies of a recombinant 25.5 kDa  glutathione transferase of Taenia solium metacestode  (rTs25GST1-1).
abstract: In this work, we studied a recombinant mu-class glutathione transferase of 25.5 kDa from Taenia solium metacestode (rTs25GST1-1) that follows Michaelis–Menten kinetics with 1-chloro-2,4-dinitrobenzene (CDNB). The kinetic parameters obtained for rTs25GST1-1 with CDNB and GSH were V(max) =12.04 μmol/min/mg and K(m)=1.38 mM, and V(max) =10.20 μmol/min/mg and K(m)=0.90, respectively. The optimal activity was found at pH 8 in the 37-40 °C temperature range. Circular dichroism studies for rTs25GST1-1 at different pH showed that it maintains a typical α-helix structure between pH 6.5-7.5, but loses it between pH 8 and 8.5. Thermal CD assays showed rTs25GST1-1 barely changed its secondary structure. Unfolding/refolding assays showed that rTs25GST1-1 retained its structure up to 40 °C without loss of its activity. Additionally, exposure of rTs25GST1-1 to cumene hydroperoxide did not produce significant changes in its structure and only affected 50% of its activity.
authors: Aramis
authors: A
authors: Roldan
authors: Anayetzin
authors: A
authors: Torres-Rivera
authors: Abraham
authors: A
authors: Landa
mesh_terms: D000818:Animals; D002942:Circular Dichroism; D004137:Dinitrochlorobenzene / Q000378:metabolism; D004795:Enzyme Stability; D005982:Glutathione Transferase / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008970:Molecular Weight; D011487:Protein Conformation; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D041201:Taenia solium / Q000201:enzymology*; D013696:Temperature
chemical_list: D004137:Dinitrochlorobenzene; D011994:Recombinant Proteins; D005982:Glutathione Transferase
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