title: Spasmolytic effects of aqueous extract of mixture from Aframomumum melegueta (K Schum) - Citrus aurantifolia (Christm and Panzer) on isolated trachea from rat.
abstract: The spasmolytic properties of the aqueous extract of Aframomum melegueta (K Schum) and Citrus aurantifolia (Christm and Panzer) (AMCA) mixture were tested on isolated rat trachea. Inhibition of the contraction was observed the in presence of the AMCA (EC 50 = 1.80 ± 0.48 mg/mL) after a pre contraction of the trachea by acetylcholine (10⁻⁵ M). With propranolol (10⁻⁶ M), the spasmolytic activity of the mixture was inhibited and the concentration-response curve shifted to the right. The EC50 value was then 2.60 ± 0.41 mg/mL. AMCA also inhibited contraction induced by KCl (4.10⁻² M) with EC50 value = 1.86 ± 0.65 mg/mL. These results clearly show the relaxing effect of the aqueous extract on the isolated rat trachea. This effect involved some β-adrenergic receptor inhibition.
authors: Laboratoire de Pharmacognosie et des Huiles Essentielles, Faculté des Sciences de la Santé, Faculté des Sciences et Techniques, Université d'Abomey Calavi 01 BP 918 Cotonou Bénin.
authors: Judith F
authors: JF
authors: Ahounou
authors: Geoffroy G
authors: GG
authors: Ouedraogo
authors: Joachim D
authors: JD
authors: Gbenou
authors: Sylvin
authors: S
authors: Ouedraogo
authors: Wilfrid Kdd
authors: WK
authors: Agbodjogbe
authors: Pierre H
authors: PH
authors: Dansou
authors: Mansourou
authors: M
authors: Moudachirou
mesh_terms: D000109:Acetylcholine; D000319:Adrenergic beta-Antagonists / Q000494:pharmacology; D000818:Animals; D002957:Citrus; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects*; D009130:Muscle, Smooth / Q000187:drug effects*; D010276:Parasympatholytics / Q000494:pharmacology*; D010936:Plant Extracts / Q000494:pharmacology*; D011189:Potassium Chloride; D011433:Propranolol; D051381:Rats; D017208:Rats, Wistar; D014132:Trachea / Q000187:drug effects*; D030521:Zingiberaceae
chemical_list: D000319:Adrenergic beta-Antagonists; D010276:Parasympatholytics; D010936:Plant Extracts; D011189:Potassium Chloride; D011433:Propranolol; D000109:Acetylcholine
keywords: Aframomum melegueta; Citrus aurantifolia; Rat trachea; bronchodilatory; extract; spasmolytic
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