title: Multilayers based on cationic nanocomplexes for co-delivery of doxorubicin and DNA.
abstract: Co-delivery of drugs and genes has synergistic advantages in many fields such as cancer treatments. In this study, we present a multilayers for co-delivery of doxorubicin (DOX) and DNA. Ferrocene-modified polyethyleneimine (PEI-Fc) is synthesized, and it can form micelles in solution with ferrocene core and PEI shell. DOX and DNA are thus incorporated into the core and shell of the micelles, respectively, to form the PEI-Fc-DOX-DNA nanocomplexes. Such cationic nanocomplexes are used to construct multilayers through layer-by-layer assembly with negatively charged dextran sulfate (DS). We show that the multilayers can release DOX, and the release can be significantly enhanced in a hydrogen peroxide condition. Moreover, the multilayers have the ability to transfect cells through a substrate-mediated mode. The (PEI-Fc-DOX-DNA/DS) multilayers can be potentially applied to the biomedical devices for cancer treatment, regenerative medicine, etc.
authors: MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou, China.
authors: Jie-kan
authors: JK
authors: Sun
authors: Ke-feng
authors: KF
authors: Ren
authors: Lei-ze
authors: LZ
authors: Zhu
authors: Jian
authors: J
authors: Ji
mesh_terms: D002412:Cations; D004247:DNA / Q000008:administration & dosage* / Q000235:genetics; D004317:Doxorubicin / Q000008:administration & dosage* / Q000633:toxicity; D016503:Drug Delivery Systems; D005296:Ferrous Compounds; D056945:Hep G2 Cells; D006801:Humans; D006861:Hydrogen Peroxide; D000075163:Metallocenes; D008823:Micelles; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D049329:Nanostructures / Q000737:chemistry / Q000648:ultrastructure; D036103:Nanotechnology; D011094:Polyethyleneimine / Q000737:chemistry; D014162:Transfection
chemical_list: D002412:Cations; D005296:Ferrous Compounds; D000075163:Metallocenes; D008823:Micelles; D004317:Doxorubicin; D011094:Polyethyleneimine; D004247:DNA; D006861:Hydrogen Peroxide; C004998:ferrocene
keywords: Co-delivery of drugs and genes; DNA; Doxorubicin; Ferrocene; Layer-by-layer assembly; Micelles