title: Determination of carbohydrates present in Saccharomyces cerevisiae using mid-infrared spectroscopy and partial least squares regression.
abstract: A fast and simple method to control variations in carbohydrate composition of Saccharomyces cerevisiae, baker's yeast, during fermentation was developed using mid-infrared (mid-IR) spectroscopy. The method allows for precise and accurate determinations with minimal or no sample preparation and reagent consumption based on mid-IR spectra and partial least squares (PLS) regression. The PLS models were developed employing the results from reference analysis of the yeast cells. The reference analyses quantify the amount of trehalose, glucose, glycogen, and mannan in S. cerevisiae. The selection and optimization of pretreatment steps of samples such as the disruption of the yeast cells and the hydrolysis of mannan and glycogen to obtain monosaccharides were carried out. Trehalose, glucose, and mannose were determined using high-performance liquid chromatography coupled with a refractive index detector and total carbohydrates were measured using the phenol-sulfuric method. Linear concentration range, accuracy, precision, LOD and LOQ were examined to check the reliability of the chromatographic method for each analyte.
authors: Institute of Chemical Technologies and Analytics, Vienna University of Technology, Getreidemarkt 9/164-UPA, 1060, Vienna, Austria.
authors: Maria R
authors: MR
authors: Plata
authors: Cosima
authors: C
authors: Koch
authors: Patrick
authors: P
authors: Wechselberger
authors: Christoph
authors: C
authors: Herwig
authors: Bernhard
authors: B
authors: Lendl
mesh_terms: D050260:Carbohydrate Metabolism; D002241:Carbohydrates / Q000032:analysis*; D005285:Fermentation; D016018:Least-Squares Analysis; D057230:Limit of Detection; D012441:Saccharomyces cerevisiae / Q000737:chemistry* / Q000378:metabolism; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*
chemical_list: D002241:Carbohydrates
references: Biochemistry. 1968 May;7(5):1855-63
references: 5689843
references: Anal Biochem. 1995 Jun 10;228(1):143-9
references: 8572270
references: Anal Biochem. 1978 May;86(1):56-64
references: 655400
references: J Bacteriol. 1980 Sep;143(3):1384-94
references: 6997270
references: Genome Res. 2003 Feb;13(2):244-53
references: 12566402
references: J Agric Food Chem. 2010 Jan 13;58(1):39-45
references: 20050702
references: Talanta. 2012 Sep 15;99:566-73
references: 22967595
references: Yeast. 1998 Oct;14(14):1297-306
references: 9802208
references: Anal Biochem. 1997 May 15;248(1):186-8
references: 9177741
references: Appl Spectrosc. 2004 Jul;58(7):804-10
references: 15282045
references: Biosystems. 2006 Feb-Mar;83(2-3):188-94
references: 16236430
references: Anal Chim Acta. 2010 Feb 5;659(1-2):258-65
references: 20103133