title: N-acetylcysteine induced quenching of red fluorescent oligonucleotide-stabilized silver nanoclusters and the application in pharmaceutical detection.
abstract: In this work, we reported a new, simple and sensitive method for determination of N-acetylcysteine (NAC) based on quenching of the red fluorescence of oligonuleotide-protected silver nanoculsters (Ag NCs) with the quantum yield of 68.3±0.3%. This method was successfully used for the assay of NAC granules presenting a linear range from 100 nM to 1200 nM (LOD of 50 nM) with minimal interferences from potential coexisting substances. It is for the first time that quenching performance of the thiol-containing compound was found to follow a non-linear Stern-Volmer profile, indicative of a complicated quenching mechanism with static quenching dominating, in which DNA-template of Ag NCs was partly replaced by NAC, as elucidated by spectral investigations. This study extended the analytical application of silver nanoclusters as well as provided a more insightful understanding of the quenching mechanism of thiol-compounds on the fluorescence of Ag NCs.
authors: Department of Chemical Biology, School of Pharmaceutical Sciences, Peking University, Beijing 100083, China.
authors: Xinyi
authors: X
authors: Wang
authors: Ruoyun
authors: R
authors: Lin
authors: Zhihan
authors: Z
authors: Xu
authors: Hongduan
authors: H
authors: Huang
authors: Limei
authors: L
authors: Li
authors: Feng
authors: F
authors: Liu
authors: Na
authors: N
authors: Li
authors: Xiaoda
authors: X
authors: Yang
mesh_terms: D000111:Acetylcysteine / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D049329:Nanostructures / Q000737:chemistry*; D009841:Oligonucleotides / Q000737:chemistry*; D004364:Pharmaceutical Preparations / Q000032:analysis*; D012834:Silver / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
chemical_list: D005456:Fluorescent Dyes; D009841:Oligonucleotides; D004364:Pharmaceutical Preparations; D012834:Silver; D000111:Acetylcysteine
keywords: Fluorescence; N-acetylcysteine; Nucleotide; Quenching; Silver nanoclusters