title: Quantitative aspects of RNA silencing in metazoans.
abstract: Small regulatory RNAs (microRNAs, siRNAs, and piRNAs) exhibit several unique features that clearly distinguish them from other known gene regulators. Their genomic organization, mode of action, and proposed biological functions raise specific questions. In this review, we focus on the quantitative aspect of small regulatory RNA biology. The original nature of these small RNAs accelerated the development of novel detection techniques and improved statistical methods and promoted new concepts that may unexpectedly generalize to other gene regulators. Quantification of natural phenomena is at the core of scientific practice, and the unique challenges raised by small regulatory RNAs have prompted many creative innovations by the scientific community.
authors: IGH du CNRS UPR 1142, 34396 Montpellier, France. asergeeva@img.ras.ru
authors: A M
authors: AM
authors: Sergeeva
authors: N
authors: N
authors: Pinzón Restrepo
authors: H
authors: H
authors: Seitz
mesh_terms: D000818:Animals; D015152:Blotting, Northern; D002874:Chromosome Mapping; D006801:Humans; D035683:MicroRNAs / Q000032:analysis / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000032:analysis / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D035683:MicroRNAs; D034741:RNA, Small Interfering