title: In vitro pharmacological and toxicological effects of norterpene peroxides isolated from the Red Sea sponge Diacarnus erythraeanus on normal and cancer cells.
abstract: Eight cyclic peroxide norterpenoids, compounds 1-8, have been isolated and characterized from the Red Sea sponge Diacarnus erythraeanus, including two new norsesterterpene derivatives (3, 4). Among these metabolites, (-)-muqubilin A (5) (nine cell lines analyzed) and the new compounds 3 and 4 (seven cell lines analyzed) displayed mean IC₅₀ growth inhibitory concentrations in vitro of <10 μM, while the remaining compounds (1, 6-8) were inactive in these cancer cell lines. Compound 5 displayed no selectivity between normal and cancer cells in terms of in vitro growth inhibition. Quantitative video microscopy analysis carried out on (-)-muqubilin A-treated cells validated the data obtained by means of the MTT colorimetric assay, while flow cytometry analysis revealed ROS production but no induction of apoptosis in cancer cells.
authors: Service de Neurochirurgie, Hôpital Erasme, ULB, Route de Lennik, 1070 Brussels, Belgium.
authors: Florence
authors: F
authors: Lefranc
authors: Genoveffa
authors: G
authors: Nuzzo
authors: Nehal Aly
authors: NA
authors: Hamdy
authors: Issa
authors: I
authors: Fakhr
authors: Laetitia
authors: L
authors: Moreno Y Banuls
authors: Gwendoline
authors: G
authors: Van Goietsenoven
authors: Guido
authors: G
authors: Villani
authors: Véronique
authors: V
authors: Mathieu
authors: Rob
authors: R
authors: van Soest
authors: Robert
authors: R
authors: Kiss
authors: Maria Letizia
authors: ML
authors: Ciavatta
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D019156:Indian Ocean; D020128:Inhibitory Concentration 50; D015394:Molecular Structure; D010545:Peroxides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D011161:Porifera / Q000737:chemistry*; D017382:Reactive Oxygen Species / Q000378:metabolism; D013729:Terpenes / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*
chemical_list: D000970:Antineoplastic Agents; D010545:Peroxides; D017382:Reactive Oxygen Species; D013729:Terpenes