title: Mutational analysis of the yeast RNA helicase Sub2p reveals conserved domains required for growth, mRNA export, and genomic stability.
abstract: Sub2p/UAP56 is a highly conserved DEAD-box RNA helicase involved in the packaging and nuclear export of mRNA/protein particles (mRNPs). In Saccharomyces cerevisiae, Sub2p is recruited to active chromatin by the pentameric THO complex and incorporated into the larger transcription-export (TREX) complex. Sub2p also plays a role in the maintenance of genome integrity as its inactivation causes severe transcription-dependent recombination of DNA. Despite the central role of Sub2p in early mRNP biology, little is known about its function. Here, we report the presence of an N-terminal motif (NTM) conserved specifically in the Sub2p branch of RNA helicases. Mutation of the NTM causes nuclear accumulation of poly(A)(+) RNA and impaired growth without affecting core helicase functions. Thus, the NTM functions as an autonomous unit. Moreover, two sub2 mutants, that are deficient in ATP binding, act in a trans-dominant negative fashion for growth and induce high recombination rates in vivo. Although wild-type Sub2p is prevented access to transcribed loci in such a background, this does not mechanistically explain the phenotype.
authors: Cyril
authors: C
authors: Saguez
authors: Fernando A
authors: FA
authors: Gonzales
authors: Manfred
authors: M
authors: Schmid
authors: Andreas
authors: A
authors: Bøggild
authors: Chrysa M
authors: CM
authors: Latrick
authors: Francisco
authors: F
authors: Malagon
authors: Andrea
authors: A
authors: Putnam
authors: Lee
authors: L
authors: Sanderson
authors: Eckhard
authors: E
authors: Jankowsky
authors: Ditlev E
authors: DE
authors: Brodersen
authors: Torben Heick
authors: TH
authors: Jensen
mesh_terms: D000251:Adenosine Triphosphatases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D015153:Blotting, Western; D002467:Cell Nucleus / Q000235:genetics; D002843:Chromatin / Q000235:genetics; D047369:Chromatin Immunoprecipitation; D042822:Genomic Instability; D009154:Mutation / Q000235:genetics*; D034443:RNA Transport / Q000235:genetics*; D012331:RNA, Fungal / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D014158:Transcription, Genetic
chemical_list: D002843:Chromatin; D012331:RNA, Fungal; D012333:RNA, Messenger; D000251:Adenosine Triphosphatases; C027900:RNA-dependent ATPase
keywords: RNA; RNA export; RNA helicase; genome stability
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