title: Isolation and Identification of Mushroom Pathogens from Agrocybe aegerita.
abstract: Agrocybe aegerita is an important mushroom cultivated in Korea, with good feel and a peculiar fragrance. A. aegerita can be cultivated throughout the year using culture bottles but is more susceptible to contamination than other mushrooms. Twenty-two pathogens were isolated from the fruiting bodies and compost of A. aegerita, and seven isolates were isolated from Pleurotus ostreatus to compare with the A. aegerita isolates, collected from Gimje, Iksan, Gunsan of Chonbuk, and Chilgok of Gyeongbuk Province in 2009. These isolates were identified based on morphological and molecular characteristics. Of the 29 isolates, 26 were identified as Trichoderma spp. and the remaining three were Aspergillus spp., Mucor spp., and Penicillium spp. A phylogenetic analysis revealed that the 26 isolates of Trichoderma were divided into four taxa, namely T. harzianum, T. pleuroticola, T. longibrachiatum, and T. atroviride. Among the Trichoderma spp., 16 isolates (55.2%) were identified as T. harzianum, six as T. pleuroticola (20.7%), two as T. longibrachiatum, and the remaining two were T. atroviride.
authors: Jeollabuk-do Agricultural Research and Extension Services, Iksan 570-704, Korea.
authors: In-Young
authors: IY
authors: Choi
authors: Jang-Nam
authors: JN
authors: Choi
authors: Praveen K
authors: PK
authors: Sharma
authors: Wang-Hyu
authors: WH
authors: Lee
keywords: Agrocybe aegerita; Phylogenetic analysis; Trichoderma spp.; rDNA internal transcribed spacer sequence
references: Mycologia. 2002 Jan-Feb;94(1):146-70
references: 21156486
references: Mol Biol Evol. 1987 Jul;4(4):406-25
references: 3447015
references: Nutrition. 2000 Jul-Aug;16(7-8):694-6
references: 10906601
references: Mol Biol Evol. 2007 Aug;24(8):1596-9
references: 17488738
references: Nucleic Acids Res. 1994 Nov 11;22(22):4673-80
references: 7984417
references: Phytother Res. 1999 Sep;13(6):529-31
references: 10479768
references: Med Mycol. 2006 Sep 1;44(Supplement_1):S133-S148
references: 30408897