title: Genetic and chemical characterization of an EMS induced mutation in Cucumis melo CRTISO gene.
abstract: In order to broaden the available genetic variation of melon, we developed an ethyl methanesulfonate mutation library in an orange-flesh 'Charentais' type melon line that accumulates β-carotene. One mutagenized M2 family segregated for a novel recessive trait, a yellow-orange fruit flesh ('yofI'). HPLC analysis revealed that 'yofI' accumulates pro-lycopene (tetra-cis-lycopene) as its major fruit pigment. The altered carotenoid composition of 'yofI' is associated with a significant change of the fruit aroma since cleavage of β-carotene yields different apocarotenoids than the cleavage of pro-lycopene. Normally, pro-lycopene is further isomerized by CRTISO (carotenoid isomerase) to yield all-trans-lycopene, which is further cyclized to β-carotene in melon fruit. Cloning and sequencing of 'yofI' CRTISO identified two mRNA sequences which lead to truncated forms of CRTISO. Sequencing of the genomic CRTISO identified an A-T transversion in 'yofI' which leads to a premature STOP codon. The early carotenoid pathway genes were up regulated in yofI fruit causing accumulation of other intermediates such as phytoene and ζ-carotene. Total carotenoid levels are only slightly increased in the mutant. Mutants accumulating pro-lycopene have been reported in both tomato and watermelon fruits, however, this is the first report of a non-lycopene accumulating fruit showing this phenomenon.
authors: Department of Vegetable Crops & Plant Genetics, Agricultural Research Organization, Newe Ya'ar Research Center, P.O. Box 1021, Ramat Yishay 30-095, Israel.
authors: Navot
authors: N
authors: Galpaz
authors: Yosi
authors: Y
authors: Burger
authors: Tamar
authors: T
authors: Lavee
authors: Galil
authors: G
authors: Tzuri
authors: Amir
authors: A
authors: Sherman
authors: Tal
authors: T
authors: Melamed
authors: Ravit
authors: R
authors: Eshed
authors: Ayala
authors: A
authors: Meir
authors: Vitaly
authors: V
authors: Portnoy
authors: Einat
authors: E
authors: Bar
authors: Einav
authors: E
authors: Shimoni-Shor
authors: Ari
authors: A
authors: Feder
authors: Yuval
authors: Y
authors: Saar
authors: Uzi
authors: U
authors: Saar
authors: Fabian
authors: F
authors: Baumkoler
authors: Efraim
authors: E
authors: Lewinsohn
authors: Arthur A
authors: AA
authors: Schaffer
authors: Nurit
authors: N
authors: Katzir
authors: Yaakov
authors: Y
authors: Tadmor
mesh_terms: D053898:Biosynthetic Pathways / Q000235:genetics; D002338:Carotenoids / Q000235:genetics; D002851:Chromatography, High Pressure Liquid; D028463:Cucumis melo / Q000737:chemistry / Q000235:genetics* / Q000254:growth & development; D005020:Ethyl Methanesulfonate / Q000737:chemistry*; D000077276:Lycopene; D016296:Mutagenesis; D019207:beta Carotene / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D019745:cis-trans-Isomerases / Q000737:chemistry / Q000235:genetics*
chemical_list: D019207:beta Carotene; D002338:Carotenoids; D005020:Ethyl Methanesulfonate; D019745:cis-trans-Isomerases; D000077276:Lycopene
keywords: Apocarotenoids; Carotenoid isomerase; Cucumis melo; Ethyl methanesulfonate; Gene expression; Pro-lycopene