title: Activation of the Akt/mTOR pathway in dentigerous cysts, odontogenic keratocysts, and ameloblastomas.
abstract: OBJECTIVE
  The aim of this study was to investigate the Akt/mTOR pathway in dentigerous cysts (DCs), odontogenic keratocysts (OKCs), and ameloblastomas.
  
  
  METHODS
  A total of 90 cases were studied (30 DCs, 30 OKCs, and 30 ameloblastomas). Patient records on age, sex, lesion location, symptoms, and radiographic and histopathologic features were collected. The phosphorylation of components of the Akt/mTOR pathway [p-Akt (Ser473), p-Akt (Thr308), and phosphorylated-ribosomal protein S6 (p-RPS6)] was studied using immunohistochemistry. Correlations with clinical features were analyzed using the Spearman rank test.
  
  
  RESULTS
  Over 90% of OKCs and ameloblastomas and 60% of DCs stained positive for p-Akt (Ser473). Phospho-Akt (Thr308) was positive in 73% of ameloblastomas, 40% of OKCs, and 20% of DCs. Phospho-RPS6 was detected most frequently in OKCs (83%), followed by ameloblastomas (76%) and DCs (53%). No correlations were noted between the immunohistochemical findings and the clinicopathologic parameters.
  
  
  CONCLUSIONS
  The Akt/mTOR pathway is upregulated in DCs, OKCs, and ameloblastomas. This pathway may be involved in the development of these lesions.
authors: Department of Oral Pathology, Developing Research Unit of Prevention and Therapy of Oral Cancers and Neoplasms by Herbal Medicine, Faculty of Dentistry, Chulalongkorn University, Pathumwan, Bangkok, Thailand.
authors: Risa
authors: R
authors: Chaisuparat
authors: Somchai
authors: S
authors: Yodsanga
authors: Silvia
authors: S
authors: Montaner
authors: Bruno C
authors: BC
authors: Jham
mesh_terms: D000293:Adolescent; D000328:Adult; D000564:Ameloblastoma / Q000378:metabolism* / Q000473:pathology; D003803:Dentigerous Cyst / Q000378:metabolism* / Q000473:pathology; D005260:Female; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D007573:Jaw Neoplasms / Q000378:metabolism* / Q000473:pathology; D020935:MAP Kinase Signaling System / Q000502:physiology*; D008297:Male; D009807:Odontogenic Cysts / Q000378:metabolism* / Q000473:pathology; D010766:Phosphorylation; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism*; D012189:Retrospective Studies; D038601:Ribosomal Protein S6 / Q000378:metabolism; D058570:TOR Serine-Threonine Kinases / Q000378:metabolism*
chemical_list: D038601:Ribosomal Protein S6; C546842:MTOR protein, human; D017346:Protein Serine-Threonine Kinases; D058570:TOR Serine-Threonine Kinases