title: Backbone ¹H, ¹⁵N, and ¹³C and side chain ¹³Cβ NMR chemical shift assignment of murine interleukin-10.
abstract: Almost complete assignment of backbone (1)H, (13)C, (15)N and side chain (13)Cβ resonances for the immune-regulatory cytokine IL-10 is reported. The protein was overexpressed in Escherichia coli and was refolded from inclusion bodies. The point mutation C149Y was introduced to suppress incorrect disulfide bond formation and to improve protein refolding.
authors: Institute of Medical Physics and Biophysics, University of Leipzig, Härtelstraße 16/18, 04107, Leipzig, Germany, Georg.Kuenze@medizin.uni-leipzig.de.
authors: Georg
authors: G
authors: Künze
authors: Stephan
authors: S
authors: Theisgen
authors: Daniel
authors: D
authors: Huster
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D016753:Interleukin-10 / Q000737:chemistry*; D051379:Mice; D019906:Nuclear Magnetic Resonance, Biomolecular
chemical_list: D016753:Interleukin-10