title: Staphylococcus saprophyticus surface-associated protein (Ssp) is associated with lifespan reduction in Caenorhabditis elegans.
abstract: Staphylococcal lipases have been proposed as pathogenicity factors. In Staphylococcus saprophyticus the surface-associated protein (Ssp) has been previously characterized as a cell wall-associated true lipase. A S. saprophyticus Δssp::ermB mutant has been described as less virulent in an in vivo model of urinary tract infection compared with its wild-type. This is the first report showing that S. saprophyticus induced a lifespan reduction in Caenorhabditis elegans similar to that of S. aureus RN4220. In two S. saprophyticus Δssp::ermB mutants lifespan reduction in C. elegans was partly abolished. In order to attribute virulence to the lipase activity itself and distinguish this phenomenon from the presence of the Ssp-protein, the conserved active site of the lipase was modified by site-directed ligase-independent mutagenesis and lipase activity-deficient mutants were constructed. These results indicate that the Ssp is associated with pathogenicity in C. elegans and one could speculate that the lipase activity itself is responsible for this virulence.
authors: Institute for Hygiene and Microbiology; Department of Medical Microbiology; Ruhr-University Bochum; Bochum, Germany.
authors: Florian
authors: F
authors: Szabados
authors: Amelie
authors: A
authors: Mohner
authors: Britta
authors: B
authors: Kleine
authors: Sören G
authors: SG
authors: Gatermann
mesh_terms: D000818:Animals; D017173:Caenorhabditis elegans / Q000382:microbiology* / Q000502:physiology*; D020134:Catalytic Domain; D004252:DNA Mutational Analysis; D017353:Gene Deletion; D008049:Lipase / Q000235:genetics / Q000378:metabolism*; D008136:Longevity; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D016297:Mutagenesis, Site-Directed; D050505:Mutant Proteins / Q000235:genetics / Q000378:metabolism; D057790:Staphylococcus saprophyticus / Q000201:enzymology* / Q000235:genetics / Q000472:pathogenicity*; D014774:Virulence; D037521:Virulence Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: D008565:Membrane Proteins; D050505:Mutant Proteins; D037521:Virulence Factors; D008049:Lipase
keywords: C. elegans; Ssp; Staphylococcus saprophyticus; lifespan reduction; lipase
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