title: Dual-micropillar-based microfluidic platform for single embryonic stem cell-derived neuronal differentiation.
abstract: We developed the dual-micropillar-based microfluidic platform to direct embryonic stem (ES) cell fate. 4 × 4 dual-micropillar-based microfluidic platform consisted of 16 circular-shaped outer micropillars and 8 saddle-shaped inner micropillars in which single ES cells were cultured. We hypothesized that dual-micropillar arrays would play an important role in controlling the shear stress and cell docking. Circular-shaped outer micropillars minimized the shear stress, whereas saddle-shaped innermicropillars allowed for docking of individual ES cells. We observed the effect of saddle-shaped inner micropillars on cell docking in response to hydrodynamic resistance. We also demonstrated that ES cells cultured for 6 days within the dual-micropillar-based microfluidic platform differentiated into neural-like cells. Therefore, this dual-micropillar-based microfluidic platform could be a potentially powerful method for screening of lineage commitments of single ES cells.
authors: Department of Bionano Technology, Hanyang University, Ansan, Korea.
authors: Jong Min
authors: JM
authors: Lee
authors: Ji-eun
authors: JE
authors: Kim
authors: Jayant
authors: J
authors: Borana
authors: Bong Hyun
authors: BH
authors: Chung
authors: Bong Geun
authors: BG
authors: Chung
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques / Q000295:instrumentation* / Q000379:methods; D002460:Cell Line; D053595:Embryonic Stem Cells / Q000166:cytology*; D051379:Mice; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods; D055495:Neurogenesis / Q000502:physiology*; D009474:Neurons / Q000166:cytology*; D059010:Single-Cell Analysis / Q000295:instrumentation* / Q000379:methods