title: Detection of cytomegalovirus DNA in plasma as an adjunct diagnostic for gastrointestinal tract disease in kidney and liver transplant recipients.
abstract: BACKGROUND
  Cytomegalovirus (CMV) disease is the most common infectious complication after solid organ transplantation, frequently affecting the gastrointestinal (GI) tract. There are limited data on quantitative polymerase chain reaction (qPCR) for plasma CMV DNA as an adjunct diagnostic method for GI tract disease in kidney and liver transplant recipients.
  
  
  METHODS
  We reviewed all records of adult kidney and liver transplant recipients with a GI tract biopsy and plasma CMV qPCR result within 15 days of biopsy during a 6.5-year period at our center. CMV GI tract disease was defined as histopathologic evidence of CMV on biopsy by immunohistochemistry or visualization of inclusion bodies.
  
  
  RESULTS
  GI tract biopsy and qPCR results were available for 81 kidney and liver transplant recipients; 20 cases of confirmed CMV GI tract disease were identified. Overall, the sensitivity of qPCR for diagnosing CMV GI tract disease was 85% (95% confidence interval [CI], 61%-96%), and the specificity was 95% (95% CI, 85%-99%). For CMV-seronegative recipients (R(-)) with CMV-seropositive donors (D(+)), the sensitivity of qPCR was 100% (95% CI, 59%-99%), and the specificity was 80% (95% CI, 30%-99%). The lowest sensitivity was observed in CMV D(+)/R(+) cases (72.7%; 95% CI, 39%-93%). The mean plasma CMV copy number in patients with GI tract disease was 3.84 log10 (38 334 copies/mL).
  
  
  CONCLUSIONS
  Plasma CMV qPCR had good sensitivity and excellent specificity for CMV GI tract disease in kidney and liver transplant recipients. Its sensitivity was 100% in CMV D(+)/R(-) cases but 72.7% in CMV D(+)/R(+) cases. This variation in assay performance according to host serostatus may reflect differences in disease pathogenesis.
authors: Department of Medicine.
authors: Christine M
authors: CM
authors: Durand
authors: Kieren A
authors: KA
authors: Marr
authors: Christina A
authors: CA
authors: Arnold
authors: Lydia
authors: L
authors: Tang
authors: Daniel J
authors: DJ
authors: Durand
authors: Robin K
authors: RK
authors: Avery
authors: Alexandra
authors: A
authors: Valsamakis
authors: Dionissios
authors: D
authors: Neofytos
mesh_terms: D000328:Adult; D000368:Aged; D015331:Cohort Studies; D003092:Colitis / Q000097:blood* / Q000175:diagnosis / Q000821:virology*; D003587:Cytomegalovirus / Q000235:genetics / Q000302:isolation & purification*; D003586:Cytomegalovirus Infections / Q000097:blood* / Q000175:diagnosis / Q000821:virology*; D004279:DNA, Viral / Q000097:blood*; D005260:Female; D006801:Humans; D016030:Kidney Transplantation; D016031:Liver Transplantation; D008297:Male; D008875:Middle Aged
chemical_list: D004279:DNA, Viral
keywords: CMV; colitis; kidney and liver transplant recipients; viremia
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