title: Culturable heterotrophic bacteria associated with healthy and bleached scleractinian Madracis decactis and the fireworm Hermodice carunculata from the remote St. Peter and St. Paul Archipelago, Brazil.
abstract: We report on the first characterization of the culturable heterotrophic bacteria of the scleractinian Madracis decactis. In addition, we characterized the culturable bacteria associated with the fireworm Hermodice carunculata, observed predating partially bleached coral colonies. Our study was carried out in the remote St. Peter and St. Paul Archipelago (SPSPA), Mid-Atlantic Ridge, Brazil. We constituted a 403 isolates collection and subsequently characterized it by means of pyrH and 16S rRNA partial sequences. We identified Photobacterium, Bacillus, and Vibrio species as members of the culturable microbiota of healthy M. decactis. V. campbellii, V. harveyi, V. communis, and V. maritimus were the most commonly found Vibrio species in healthy corals, representing more than 60 % of all vibrio isolates. Most of the vibrios isolated from the fireworm's tissues (n = 143; >90 %) were identified as V. shiloi. However, we did not recover V. shiloi from bleached M. decactis. Instead, we isolated V. communis, a novel Photobacterium species, Bacillus, Kocuria, and Pseudovibrio, suggesting a possible role of other facultative anaerobic bacteria and/or environmental features (such as water quality) in the onset of bleaching in SPSPA's M. decactis.
authors: Laboratory of Microbiology, Institute of Biology, Federal University of Rio de Janeiro (UFRJ), Av. Carlos Chagas Fo. S/N - CCS - IB - BIOMAR - Laboratório de Microbiologia - Bloco A (Anexo) A3 - sl 102 - Cidade Universitária, Rio de Janeiro, RJ, 21941-599, Brazil, anapaulabm@biologia.ufrj.br.
authors: Ana Paula B
authors: AP
authors: Moreira
authors: Luciane A Chimetto
authors: LA
authors: Tonon
authors: Cecilia do Valle P
authors: Cdo V
authors: Pereira
authors: Nelson
authors: N
authors: Alves
authors: Gilberto M
authors: GM
authors: Amado-Filho
authors: Ronaldo Bastos
authors: RB
authors: Francini-Filho
authors: Rodolfo
authors: R
authors: Paranhos
authors: Fabiano L
authors: FL
authors: Thompson
mesh_terms: D000818:Animals; D037421:Anthozoa / Q000382:microbiology*; D001156:Artemia / Q000382:microbiology / Q000502:physiology; D001419:Bacteria / Q000145:classification* / Q000302:isolation & purification*; D001938:Brazil; D010802:Phylogeny; D011077:Polychaeta / Q000382:microbiology*; D014733:Vibrio / Q000145:classification / Q000302:isolation & purification / Q000472:pathogenicity
references: Int J Syst Evol Microbiol. 2003 Jan;53(Pt 1):309-315
references: 12656189
references: Appl Environ Microbiol. 2005 Sep;71(9):5107-15
references: 16151093
references: Int J Syst Evol Microbiol. 2004 Nov;54(Pt 6):2307-2312
references: 15545476
references: Syst Appl Microbiol. 2001 Dec;24(4):520-38
references: 11876360
references: ISME J. 2011 Sep;5(9):1471-83
references: 21451583
references: Appl Environ Microbiol. 1998 Apr;64(4):1379-84
references: 16349542
references: Int J Syst Evol Microbiol. 2013 May;63(Pt 5):1880-1884
references: 23002050
references: Arch Microbiol. 2008 Aug;190(2):185-94
references: 18523756
references: FEMS Microbiol Ecol. 2006 Oct;58(1):99-108
references: 16958911
references: Mol Biol Evol. 1987 Jul;4(4):406-25
references: 3447015
references: Environ Microbiol Rep. 2010 Feb;2(1):90-5
references: 23766002
references: Environ Microbiol. 2005 Sep;7(9):1488-95
references: 16104871
references: J Appl Microbiol. 2009 Jun;106(6):1818-26
references: 19291243
references: Int J Syst Evol Microbiol. 2005 Mar;55(Pt 2):913-917
references: 15774685
references: Appl Environ Microbiol. 2000 Jul;66(7):3031-6
references: 10877802
references: Nat Rev Microbiol. 2007 May;5(5):355-62
references: 17384666
references: Dis Aquat Organ. 2006 Mar 23;69(1):1-7
references: 16703761
references: Mol Biol Evol. 2011 Oct;28(10):2731-9
references: 21546353
references: FEMS Microbiol Lett. 2007 Nov;276(1):106-13
references: 17937669
references: Nihon Saikingaku Zasshi. 1963 Nov;18:387-92
references: 14115910
references: Environ Microbiol. 2010 May;12(5):1122-31
references: 20132278
references: Appl Environ Microbiol. 2006 Aug;72(8):5254-9
references: 16885273
references: Mar Pollut Bull. 2012 May;64(5):1074-8
references: 22482868
references: Mol Ecol. 2013 Apr;22(8):2280-91
references: 23517352
references: PLoS One. 2011;6(7):e22298
references: 21799816
references: ISME J. 2008 Apr;2(4):350-63
references: 18059490
references: Int J Syst Evol Microbiol. 2013 Mar;63(Pt 3):982-988
references: 22685102
references: PLoS One. 2008 Feb 27;3(2):e1584
references: 18301735
references: Syst Appl Microbiol. 2008 Sep;31(4):312-9
references: 18678453
references: Mol Ecol. 2010 May;19(9):1978-90
references: 20529072
references: Int J Syst Evol Microbiol. 2011 Feb;61(Pt 2):362-368
references: 20305064
references: Int J Syst Evol Microbiol. 2001 Jul;51(Pt 4):1383-1388
references: 11491336
references: Environ Microbiol. 2003 Apr;5(4):250-5
references: 12662172
references: Annu Rev Microbiol. 2004;58:143-59
references: 15487933
references: PLoS One. 2012;7(6):e36687
references: 22679480
references: Environ Microbiol. 2010 Sep;12(9):2479-85
references: 20406294
references: Environ Microbiol. 2005 Aug;7(8):1162-74
references: 16011753
references: Environ Microbiol. 2007 Jul;9(7):1851-7
references: 17564618