title: Increased frequency of T cells expressing IL-10 in Alzheimer disease but not in late-onset depression patients.
abstract: Higher risk of dementia is expected for patients with late onset depression (LOD) history. The IL-10 polymorphisms are associated with Alzheimer disease (AD). On the other hand, there is no study associating IL-10 polymorphisms to LOD. This study aimed to investigate the -1082G/A polymorphism association in LOD, AD patients and controls and also the peripheral expression of IL-10 in CD4+ T cells. It was done in a case-control study comparing immune system phenotype and genetic polymorphism association among control individuals, LOD and AD patients. Participants were 569 subjects composed the genetics sample (249 AD, 222 LOD and 98 controls) from a tertiary medical center based on Belo Horizonte, Brazil. Flow cytometry analysis was performed in 55 people (22 AD patients, 11 LOD patients and 22 controls). A real time PCR for IL-10 SNP (rs 1800896) through genotyping analysis and flow cytometry evaluation of CD4+ T cells expressing IL-10 was done. An increased CD4+ T cells expressing IL-10 were detected only in the AD group. There was no difference detected in allele or genotype analysis for IL-10 polymorphism among LOD, AD patients or controls. IL-10 might have a role in the modulation of immune response in AD patients, however it is not presented in LOD population.
authors: INCT de Medicina Molecular, Faculdade de Medicina, Universidade Federal de Minas Gerais, Avenida Alfredo Balena, 190, Belo Horizonte, MG CEP 30130-100, Brazil. Electronic address: kcltorres@gmail.com.
authors: Karen Cecília
authors: KC
authors: Torres
authors: Patrícia
authors: P
authors: Araújo Pereira
authors: Giselle Sabrina
authors: GS
authors: Lima
authors: Isadora Cristina
authors: IC
authors: Bozzi
authors: Vitor Bortolo
authors: VB
authors: Rezende
authors: Maria Aparecida
authors: MA
authors: Bicalho
authors: Edgar Nunes
authors: EN
authors: Moraes
authors: Débora Marques
authors: DM
authors: Miranda
authors: Marco Aurélio
authors: MA
authors: Romano-Silva
mesh_terms: D017668:Age of Onset; D000368:Aged; D000369:Aged, 80 and over; D000544:Alzheimer Disease / Q000235:genetics / Q000473:pathology*; D015704:CD4 Antigens / Q000378:metabolism; D002478:Cells, Cultured; D003863:Depression / Q000235:genetics / Q000473:pathology*; D005260:Female; D005434:Flow Cytometry; D005786:Gene Expression Regulation / Q000235:genetics; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D016753:Interleukin-10 / Q000235:genetics / Q000378:metabolism*; D008297:Male; D009000:Monocytes / Q000378:metabolism; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D013601:T-Lymphocytes / Q000378:metabolism*
chemical_list: D015704:CD4 Antigens; D016753:Interleukin-10
keywords: AD; Ab; Alzheimer disease; BBB; COPD; GDS; Geriatric Depression Scale; IL-10; IL-10 in CD4+ T cells; IL-10 polymorphism; LOD; Late-Onset Depression; MMSE; Mini-mental State Examination; PCR; SNP; beta amyloid peptide; blood–brain-barrier; chronic obstructive pulmonary disorder; interleukin 10; late onset depression; polymerase chain reaction; single nucleotide polymorphism