title: A new system to measure and compare hepatitis C virus replication capacity using full-length, replication competent viruses.
abstract: Measuring the in vitro replication capacity of viruses is an important tool for assessing the effects of selective pressure of immune responses and drug therapy. Measuring hepatitis C virus (HCV) replication capacity utilizing primarily sub-genomic reporter constructs is limited. To overcome some of these limitations a quantitative reverse transcriptase PCR (RT-qPCR) was designed to measure simultaneously the growth rate of 2 whole genome HCV variants under identical culture conditions. The assay demonstrates 100% specificity of detection of each variant and a linear detection range from 200 to 2×10(8) copies. The system was validated using a panel of HCV mutants, including the NS3 protease inhibitor drug resistance mutants R155K and T54A. The creation of a unique sequence tag results in highly sensitive and specific discrimination of parental JFH-FNX and modified clones using distinct probes in a RT-qPCR allowing for comparison of the effect of drug resistance or immune escape mutations on HCV replication. This system has advantages over existing methods both by permitting direct comparison of the replication capacity of fully replication-competent HCV mutants under identical culture conditions and by measuring effects on replication capacity due to mutations affecting all stages of the viral life cycle including entry and egress.
authors: Division of Infectious Diseases, David Geffen School of Medicine at UCLA, Los Angeles, CA 90095, USA.
authors: Britta
authors: B
authors: Lassmann
authors: Vaithilingaraja
authors: V
authors: Arumugaswami
authors: Kara W
authors: KW
authors: Chew
authors: Martha J
authors: MJ
authors: Lewis
mesh_terms: D024882:Drug Resistance, Viral; D016679:Genome, Viral; D016174:Hepacivirus / Q000235:genetics / Q000502:physiology*; D057131:Immune Evasion; D009154:Mutation; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D014773:Virology / Q000379:methods*; D014779:Virus Replication
keywords: Drug resistance; Fitness; HCV; Immune escape; Replicon