title: Data-driven sample size determination for metabolic phenotyping studies.
abstract: Sample size determination is a key question in the experimental design of medical studies. The number of patients to include in a clinical study is actually critical to evaluate costs and inclusion requirements to achieve a sufficient statistical power of test and the identification of significant variations among the factors under study. Metabolic phenotyping is an expanding field of translational research in medicine, focusing on the identification of metabolism rearrangements due to various pathophysiological conditions. This top-down hypothesis-free approach uses analytical chemistry methods, coupled to statistical analysis, to quantify subtle and coordinated metabolite concentration variations and eventually identify candidate biomarkers. The sample size determination in metabolic phenotyping studies is difficult considering the absence of a priori metabolic target. This technical note introduces a data-driven sample size determination for metabolic phenotyping studies. Starting from nuclear magnetic resonance (NMR) spectra belonging to a small cohort, metabolic NMR variables are identified by the statistical recoupling of variables (SRV) procedure. A larger data set is then generated on the basis of Kernel density estimation of SRV variable distributions. Statistically significant variations of metabolic NMR signals identified by SRV are assessed by the Benjamini-Yekutieli correction for simulated data sets of variable sizes. Simulated model robustness is evaluated by receiver operating characteristic analysis (sensitivity and specificity) on an independent cohort and cross-validation. Sample size determination is obtained by identifying the optimal data set size, depending on the purpose of the study: at least one statistically significant variation (biomarker discovery) or a maximum of statistically significant variations (metabolic exploration).
authors: Hospices Civils de Lyon, Département de réanimation néonatale et néonatalogie, Hôpital Femme Mère Enfant, 59 bd Pinel, Bron Cedex, Bron 69677, France.
authors: Benjamin J
authors: BJ
authors: Blaise
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000097:blood; D017173:Caenorhabditis elegans / Q000235:genetics; D029742:Caenorhabditis elegans Proteins / Q000235:genetics; D015331:Cohort Studies; D006801:Humans; D008279:Magnetic Resonance Imaging; D008660:Metabolism / Q000235:genetics*; D010641:Phenotype; D015203:Reproducibility of Results; D018401:Sample Size; D013482:Superoxide Dismutase / Q000235:genetics
chemical_list: D015415:Biomarkers; D029742:Caenorhabditis elegans Proteins; C587809:Sod-1 protein, C elegans; D013482:Superoxide Dismutase