title: Identification of regioisomers and enantiomers of triacylglycerols in different yeasts using reversed- and chiral-phase LC-MS.
abstract: LC with atmospheric pressure chemical ionization (ACPI) MS with RP and chiral phase was used for separation of triacylglycerols (TAGs) from yeasts of the genera Candida, Kluyveromyces, Rhodotorula, Saccharomyces, Torulospora, Trichosporon, and Yarrowia. Chiral LC-APCI-MS is based on using two columns in series packed with a 3,5-dimethylphenyl carbamate modified β-cyclodextrin chiral phase. All regioisomers and enantiomers of TAGs containing one to five double bonds were separated. Molecular species of TAGs, i.e. regioisomers and enantiomers, were identified and quantified by MS/MS. Among the 94 identified TAGs, the most abundant were triolein, oleopalmitoleoolein, and dipalmitoleoolein. In strains producing palmitoleic acid in amounts >25% of total fatty acids (FAs), this acid, or unsaturated FA is bound in sn-1. In strains containing palmitoleic acid at 10-25% total FAs this acid is mainly bound in sn-3, saturated FA being bound in sn-1. Strains containing <10% palmitoleic acid form preferentially symmetrical TAGs.
authors: Institute of Microbiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic.
authors: Tomáš
authors: T
authors: Rezanka
authors: Irena
authors: I
authors: Kolouchová
authors: Alena
authors: A
authors: Cejková
authors: Tomáš
authors: T
authors: Cajthaml
authors: Karel
authors: K
authors: Sigler
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D056148:Chromatography, Reverse-Phase / Q000379:methods; D013237:Stereoisomerism; D014280:Triglycerides / Q000737:chemistry* / Q000378:metabolism; D015003:Yeasts / Q000737:chemistry* / Q000145:classification / Q000378:metabolism
chemical_list: D014280:Triglycerides
keywords: Atmospheric pressure chemical ionization mass spectrometry; Chiral LC; Reversed phase LC; Triacylglycerols; Yeast