title: Mitochondrial ferritin is a functional iron-storage protein in cucumber (Cucumis sativus) roots.
abstract: In plants, intracellular Fe trafficking must satisfy chloroplasts' and mitochondrial demands for Fe without allowing its accumulation in the organelles in dangerous redox-active forms. Protein ferritin is involved in such homeostatic control, however its functional role in mitochondria, differently from its role in chloroplasts, is still matter of debate. To test ferritin functionality as a 24-mer Fe-storage complex in mitochondria, cucumber seedlings were grown under different conditions of Fe supply (excess, control, deficiency) and mitochondria were purified from the roots. A ferritin monomer of around 25 KDa was detected by SDS-PAGE in Fe-excess root mitochondria, corresponding to the annotated Csa5M215130/XP_004163524 protein: such a monomer is barely detectable in the control mitochondria and not at all in the Fe-deficient ones. Correspondingly, the ferritin 24-mer complex is abundant in root mitochondria from Fe-excess plants and it stores Fe as Fe(III): such a complex is also detectable, though to a much smaller extent, in control mitochondria, but not in Fe-deficient ones. Cucumber ferritin Csa5M215130/XP_004163524 is therefore a functional Fe(III)-store in root mitochondria and its abundance is dependent on the Fe nutritional status of the plant.
authors: Dipartimento di Scienze Agrarie e Ambientali - Produzione, Territorio, Agroenergia, Università degli Studi di Milano Milano, Italy.
authors: Gianpiero
authors: G
authors: Vigani
authors: Delia
authors: D
authors: Tarantino
authors: Irene
authors: I
authors: Murgia
keywords: Cucumis sativus; O2 consumption; ferritin; iron homeostasis; iron-storage protein; micronutrients; mitochondria; roots
references: Plant Physiol. 1986 Oct;82(2):511-7
references: 16665060
references: Plant Sci. 2011 Nov;181(5):582-92
references: 21893255
references: Nat Protoc. 2007;2(4):953-71
references: 17446895
references: Plant Physiol Biochem. 2007 Dec;45(12):898-907
references: 17980612
references: New Phytol. 2009;182(1):127-136
references: 19192186
references: Biochim Biophys Acta. 2001 Dec 12;1541(1-2):54-63
references: 11750662
references: PLoS One. 2011;6(7):e22728
references: 21829493
references: Front Plant Sci. 2013 May 31;4:169
references: 23755060
references: Nucleic Acids Res. 2012 Jan;40(Database issue):D1178-86
references: 22110026
references: Hum Mol Genet. 2009 Jan 1;18(1):1-11
references: 18815198
references: Trends Plant Sci. 2012 Jan;17(1):47-55
references: 22093370
references: J Plant Physiol. 2010 May 15;167(8):666-9
references: 20116882
references: J Mol Biol. 2007 Jun 1;369(2):356-67
references: 17433818
references: Nucleic Acids Res. 1988 Nov 25;16(22):10881-90
references: 2849754
references: Trends Plant Sci. 2013 Jun;18(6):305-11
references: 23462548
references: BMC Genomics. 2011 Nov 02;12:540
references: 22047402
references: PLoS One. 2011;6(7):e22325
references: 21799823
references: J Biol Chem. 2001 Feb 23;276(8):5584-90
references: 11092880
references: Proteins. 1991;11(2):95-110
references: 1946347
references: EMBO J. 2004 May 19;23(10):2105-15
references: 15103330
references: Plant Cell. 2001 Aug;13(8):1803-18
references: 11487694
references: Biochim Biophys Acta. 2009 Jul;1790(7):589-99
references: 18929623
references: J Plant Physiol. 2010 Apr 15;167(6):453-60
references: 19959254
references: Microbiologyopen. 2012 Jun;1(2):95-104
references: 22950017
references: Eur J Biochem. 2004 Sep;271(18):3657-64
references: 15355342
references: J Biol Chem. 2010 Apr 16;285(16):11948-57
references: 20159981
references: J Biol Chem. 2006 Aug 18;281(33):23579-88
references: 16782706
references: Toxicol Lett. 1995 Dec;82-83:969-74
references: 8597169
references: Annu Rev Plant Biol. 2012;63:131-52
references: 22404471
references: J Biol Chem. 2001 Jul 6;276(27):24437-40
references: 11323407
references: J Plant Physiol. 2012 Jan 1;169(1):1-11
references: 22050893
references: New Phytol. 2011 Oct;192(1):6-12
references: 21883238
references: FEBS Lett. 1999 Dec 10;463(1-2):151-4
references: 10601657
references: Curr Opin Plant Biol. 2013 Jun;16(3):322-7
references: 23415557
references: Mol Plant. 2011 May;4(3):464-76
references: 21447758
references: Biochim Biophys Acta. 2013 Feb;1833(2):253-9
references: 22683762
references: J Plant Physiol. 2010 May 15;167(8):620-4
references: 20045215
references: Nat Genet. 2009 Dec;41(12):1275-81
references: 19881527
references: J Plant Physiol. 2010 Dec 15;167(18):1598-605
references: 20724023
references: Mol Plant. 2009 Nov;2(6):1298-309
references: 19995731
references: Plant Physiol. 2009 Feb;149(2):1099-110
references: 19109413
references: Plant J. 2002 Jun;30(5):521-8
references: 12047627
references: Biochim Biophys Acta. 2010 Aug;1800(8):806-14
references: 20026187
references: Ann Bot. 2010 May;105(5):811-22
references: 19482877
references: Eur J Biochem. 1996 Nov 1;241(3):779-86
references: 8944766