title: Quality evaluation of commercial Huang-Lian-Jie-Du-Tang based on simultaneous determination of fourteen major chemical constituents using high performance liquid chromatography.
abstract: Huang-Lian-Jie-Du-Tang (HLJDT), comprising Coptidis Rhizoma, Scutellariae Radix, Phellodendri Cortex and Gardeniae Fructus, is one of the commonly used Chinese medicine formulas for clearing heat and detoxifying. Quality control of the herbal complex like Chinese medicine formulas still remains a challenge. The successful approval of botanical drug Veregen by FDA indicated the importance of quantitative analysis in quality control of herbal medicines. In this study, an effective quantitative method based on conventional HPLC-DAD was developed for simultaneous determination of fourteen major ingredients (seven alkaloids, four flavonoids, three terpenes) in HLJDT. The established method was well validated in terms of linearity, sensitivity, precision, accuracy and stability and then successfully applied to quality evaluation of commercial HLJDT samples. The developed method can quantitatively determine up to 70% of the chemicals of commercial HLJDT sample and effectively revealed the significant variation in the quality of the commercial HLJDT samples collected from different locations.
authors: School of Chinese Medicine, Hong Kong Baptist University, Hong Kong SAR, China.
authors: Ka-Yan
authors: KY
authors: Kwok
authors: Jun
authors: J
authors: Xu
authors: Hing-Man
authors: HM
authors: Ho
authors: Hu-Biao
authors: HB
authors: Chen
authors: Min
authors: M
authors: Li
authors: Yan
authors: Y
authors: Lang
authors: Quan-Bin
authors: QB
authors: Han
mesh_terms: D000470:Alkaloids / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000032:analysis* / Q000592:standards; D005419:Flavonoids / Q000032:analysis; D013729:Terpenes / Q000032:analysis
chemical_list: D000470:Alkaloids; D004365:Drugs, Chinese Herbal; D005419:Flavonoids; D013729:Terpenes; C061676:oren gedoku to
keywords: Chinese medicine formula; Huang-Lian-Jie-Du-Tang; Quality evaluation; Quantitative analysis; Simultaneous determination