title: A highly redundant gene network controls assembly of the outer spore wall in S. cerevisiae.
abstract: The spore wall of Saccharomyces cerevisiae is a multilaminar extracellular structure that is formed de novo in the course of sporulation. The outer layers of the spore wall provide spores with resistance to a wide variety of environmental stresses. The major components of the outer spore wall are the polysaccharide chitosan and a polymer formed from the di-amino acid dityrosine. Though the synthesis and export pathways for dityrosine have been described, genes directly involved in dityrosine polymerization and incorporation into the spore wall have not been identified. A synthetic gene array approach to identify new genes involved in outer spore wall synthesis revealed an interconnected network influencing dityrosine assembly. This network is highly redundant both for genes of different activities that compensate for the loss of each other and for related genes of overlapping activity. Several of the genes in this network have paralogs in the yeast genome and deletion of entire paralog sets is sufficient to severely reduce dityrosine fluorescence. Solid-state NMR analysis of partially purified outer spore walls identifies a novel component in spore walls from wild type that is absent in some of the paralog set mutants. Localization of gene products identified in the screen reveals an unexpected role for lipid droplets in outer spore wall formation.
authors: Department of Biochemistry and Cell Biology, Stony Brook University, Stony Brook, New York, United States of America.
authors: Coney Pei-Chen
authors: CP
authors: Lin
authors: Carey
authors: C
authors: Kim
authors: Steven O
authors: SO
authors: Smith
authors: Aaron M
authors: AM
authors: Neiman
mesh_terms: D002473:Cell Wall / Q000235:genetics* / Q000378:metabolism; D048271:Chitosan / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal; D053263:Gene Regulatory Networks; D050356:Lipid Metabolism / Q000235:genetics*; D009682:Magnetic Resonance Spectroscopy; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000254:growth & development; D013172:Spores, Fungal / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D014443:Tyrosine / Q000031:analogs & derivatives / Q000235:genetics / Q000378:metabolism
chemical_list: D014443:Tyrosine; D048271:Chitosan; C007543:dityrosine
references: Proc Natl Acad Sci U S A. 2002 Nov 26;99(24):15530-5
references: 12432101
references: Yeast. 2002 Mar 30;19(5):403-22
references: 11921089
references: Eur J Biochem. 1996 Jul 1;239(1):124-31
references: 8706696
references: Genetics. 2011 Nov;189(3):737-65
references: 22084423
references: Eukaryot Cell. 2004 Dec;3(6):1464-75
references: 15590821
references: J Cell Biol. 1970 Mar;44(3):688-92
references: 4190070
references: Semin Cell Dev Biol. 1999 Oct;10(5):555-9
references: 10597640
references: Mol Gen Genet. 1974;131(4):281-9
references: 4612332
references: Mol Cell. 2008 Dec 26;32(6):878-87
references: 19111667
references: PLoS One. 2009 Sep 25;4(9):e7184
references: 19779569
references: Curr Biol. 2005 Nov 22;15(22):2056-62
references: 16303567
references: Proc Natl Acad Sci U S A. 1994 May 10;91(10):4524-8
references: 8183942
references: Yeast. 1992 Dec;8(12):1089-99
references: 1293886
references: J Cell Biol. 2001 Nov 26;155(5):797-808
references: 11724821
references: Eukaryot Cell. 2007 May;6(5):855-67
references: 17400891
references: Genetics. 2012 Nov;192(3):775-818
references: 23135325
references: Antimicrob Agents Chemother. 2008 Oct;52(10):3718-24
references: 18663031
references: Genes Dev. 1990 Oct;4(10):1775-89
references: 2249774
references: Hepatology. 2008 Jun;47(6):1936-46
references: 18393390
references: Curr Biol. 2001 Jul 10;11(13):1001-9
references: 11470404
references: Yeast. 1998 Jul;14(10):953-61
references: 9717241
references: Science. 2004 Feb 27;303(5662):1367-70
references: 14752166
references: Genetics. 2003 Jan;163(1):47-54
references: 12586695
references: J Am Chem Soc. 2008 Apr 30;130(17):5618-9
references: 18393418
references: Arch Microbiol. 1978 Apr 27;117(1):73-7
references: 354548
references: Curr Opin Microbiol. 2000 Aug;3(4):354-8
references: 10972493
references: Curr Biol. 2005 Sep 20;15(18):1663-9
references: 16169489
references: Eukaryot Cell. 2011 Sep;10(9):1264-8
references: 21784998
references: Nature. 1997 Jun 12;387(6634):708-13
references: 9192896
references: Chem Biol. 2008 Oct 20;15(10):1116-24
references: 18940671
references: Science. 1998 Oct 23;282(5389):699-705
references: 9784122
references: Genome Res. 2003 Nov;13(11):2498-504
references: 14597658
references: FEMS Microbiol Rev. 2002 Aug;26(3):239-56
references: 12165426
references: Infect Immun. 1995 Oct;63(10):4078-83
references: 7558322
references: FEBS Lett. 1999 Oct 29;460(2):275-9
references: 10544249
references: Genetics. 2000 Mar;154(3):1389-401
references: 10757778
references: Lancet. 2003 Oct 4;362(9390):1142-51
references: 14550704
references: Methods Enzymol. 1991;194:110-31
references: 2005782
references: Science. 2004 Feb 6;303(5659):808-13
references: 14764870
references: Trends Genet. 1993 Nov;9(11):395-9
references: 8310537
references: J Biol Chem. 1986 Mar 25;261(9):4288-94
references: 3512567
references: J Cell Biol. 1998 Jan 12;140(1):29-37
references: 9425151
references: J Biol Chem. 1988 Aug 15;263(23):11569-74
references: 3042773
references: Science. 1999 Aug 6;285(5429):901-6
references: 10436161
references: Appl Microbiol Biotechnol. 2012 Feb;93(3):931-40
references: 22173481
references: Yeast. 1999 Oct;15(14):1541-53
references: 10514571
references: Proc Natl Acad Sci U S A. 2007 Dec 26;104(52):20890-5
references: 18093937
references: Biochim Biophys Acta. 1996 Mar 15;1289(2):217-20
references: 8600976
references: J Mol Biol. 1990 Oct 5;215(3):403-10
references: 2231712
references: J Biol Chem. 1990 Sep 5;265(25):15118-23
references: 2203769
references: Mod Pathol. 2010 Mar;23(3):480-92
references: 20081801
references: PLoS One. 2008 Aug 06;3(8):e2873
references: 18682732
references: Gene. 1999 Sep 30;238(1):253-61
references: 10571001
references: Eukaryot Cell. 2002 Oct;1(5):799-810
references: 12455697