title: Dynamic investigation of interaction of biocompatible iron oxide nanoparticles with epithelial cells for biomedical applications.
abstract: Magnetic nanoparticles have emerged as important players in current research in modern medicine since they can be used in medicine for diagnosis and/or therapeutic treatment of diseases. Among many therapeutic applications of iron-based nanoparticles, drug delivery and photothermal therapy are of particular interest. At cellular level their uptake has been studied and the mechanism by which nanoparticles enter into the cell has important implication not only for their fate but also for their impact on the biological systems. We present here a dynamic investigation of interaction of biocompatible iron oxide nanoparticles coated with L-3,4-dihydroxyphenylalanine and labeled with tetra-methylrhodamine-5/6-isothiocyanate with lung epithelial cells. Our data show that after macropinocytosis-mediated internalization, nanoparticles in form of vesicles approach the nucleus and converge in the more acidic compartments of the cells in a microtubule-dependent manner. During progression the nanoparticles aggregate. Finally, we have demonstrated that a converging laser radiation on the cells, causes the increase in the local temperature and thus damages the cells, suggesting that these nanoparticles may be applied for photothermal therapy studies.
authors: 1 Department of Health Science, New Square of the University, 1-20126, Milan, Italy.
authors: Alice
authors: A
authors: Panariti
authors: Barbara
authors: B
authors: Lettiero
authors: Rodica
authors: R
authors: Alexandrescu
authors: Maddalena
authors: M
authors: Collini
authors: Laura
authors: L
authors: Sironi
authors: Munish
authors: M
authors: Chanana
authors: Ion
authors: I
authors: Morjan
authors: Dayan
authors: D
authors: Wang
authors: Giuseppe
authors: G
authors: Chirico
authors: Giuseppe
authors: G
authors: Miserocchi
authors: Cecilia
authors: C
authors: Bucci
authors: Ilaria
authors: I
authors: Rivolta
mesh_terms: D001672:Biocompatible Materials / Q000008:administration & dosage / Q000737:chemistry; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004847:Epithelial Cells / Q000737:chemistry* / Q000187:drug effects*; D005290:Ferric Compounds / Q000008:administration & dosage / Q000737:chemistry; D006801:Humans; D058185:Magnetite Nanoparticles / Q000008:administration & dosage* / Q000737:chemistry*; D008422:Materials Testing; D053768:Metal Nanoparticles / Q000008:administration & dosage / Q000737:chemistry; D013347:Subcellular Fractions / Q000737:chemistry* / Q000187:drug effects*
chemical_list: D001672:Biocompatible Materials; D005290:Ferric Compounds; D058185:Magnetite Nanoparticles; C000499:ferric oxide