title: DMSO enhances electrospray response, boosting sensitivity of proteomic experiments.
abstract: We report that low percentages of dimethylsulfoxide (DMSO) in liquid chromatography solvents lead to a strong enhancement of electrospray ionization of peptides, improving the sensitivity of protein identification in bottom-up proteomics by up to tenfold. The method can be easily implemented on any LC-MS/MS system without modification to hardware or software and at no additional cost.
authors: Chair of Proteomics and Bioanalytics, Center of Life and Food Sciences Weihenstephan, Technische Universität München, Freising, Germany.
authors: Hannes
authors: H
authors: Hahne
authors: Fiona
authors: F
authors: Pachl
authors: Benjamin
authors: B
authors: Ruprecht
authors: Stefan K
authors: SK
authors: Maier
authors: Susan
authors: S
authors: Klaeger
authors: Dominic
authors: D
authors: Helm
authors: Guillaume
authors: G
authors: Médard
authors: Matthias
authors: M
authors: Wilm
authors: Simone
authors: S
authors: Lemeer
authors: Bernhard
authors: B
authors: Kuster
mesh_terms: D004121:Dimethyl Sulfoxide / Q000737:chemistry*; D010455:Peptides / Q000032:analysis* / Q000737:chemistry; D040901:Proteomics / Q000379:methods* / Q000592:standards; D012680:Sensitivity and Specificity; D012997:Solvents / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods* / Q000592:standards; D053719:Tandem Mass Spectrometry / Q000379:methods* / Q000592:standards
chemical_list: D010455:Peptides; D012997:Solvents; D004121:Dimethyl Sulfoxide
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