title: Structure-activity relationships of αs-casein peptides with multifunctional biological activities.
abstract: Multifunctional bioactive peptides have a wider role in modulating physiological functions and possess multiple biological activities. Peptides from bovine milk with sequences QKALNEINQF [p10] and TKKTKLTEEEKNRL [p14] from α-S2 casein f (79-88) and α-S2 casein f (148-161) were identified to be having multifunctional biological activities and were synthesized. These synthesized peptides show various biological activities like angiotensin-converting enzyme inhibition, prolyl endopeptidase inhibition, antioxidant, and antimicrobial activities. The mode of antimicrobial mechanism was studied and p10 shows depolarization of cell membrane, whereas p14 was found to display DNA-binding activity. Structural studies envisaged backbone flexibility, for differences in their mode of action. Peptide structure function studies were correlated to understand their multifunctional biological activity.
authors: Department of Protein Chemistry and Technology, Central Food Technological Research Institute (CSIR), Mysore, 570020, Karnataka, India, sistlasrinivas@gmail.com.
authors: Srinivas
authors: S
authors: Sistla
mesh_terms: D000806:Angiotensin-Converting Enzyme Inhibitors; D000818:Animals; D023181:Antimicrobial Cationic Peptides / Q000378:metabolism*; D000975:Antioxidants; D001409:Bacillus cereus / Q000187:drug effects; D002364:Caseins / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000378:metabolism; D016480:Helicobacter pylori / Q000187:drug effects; D008089:Listeria monocytogenes / Q000187:drug effects; D008826:Microbial Sensitivity Tests; D008892:Milk / Q000378:metabolism*; D000085822:Prolyl Oligopeptidases; D017433:Protein Structure, Secondary; D012697:Serine Endopeptidases / Q000378:metabolism*; D013211:Staphylococcus aureus / Q000187:drug effects; D013329:Structure-Activity Relationship
chemical_list: D000806:Angiotensin-Converting Enzyme Inhibitors; D023181:Antimicrobial Cationic Peptides; D000975:Antioxidants; D002364:Caseins; D004268:DNA-Binding Proteins; D012697:Serine Endopeptidases; D000085822:Prolyl Oligopeptidases
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