title: Direct characterization of bulk samples by internal extractive electrospray ionization mass spectrometry.
abstract: A straight-forward analytical strategy called internal extractive electrospray ionization mass spectrometry (iEESI-MS), which combines solvent extraction of chemicals inside a bulk sample with in situ electrospray ionization mass spectrometry, has been established to directly characterize the interior of a bulk sample with molecular specificity. The method allows both qualitative and quantitative analysis of analytes distributed in a 3-dimensional volume (e.g., 1 ~ 100 mm(3)) of various synthetic and biological matrices (e.g., chewing gum, leaves, fruits, roots, pork, lung tissues) without either mashing the sample or matrix separation. Using different extraction solvents, online chromatographic separation of chemicals inside the sample volume was observed during iEESI-MS analysis. The presented method is featured by the high speed of analysis, high sensitivity, low sample consumption and minimal sample preparation and/or degradation, offering unique possibilities for advanced applications in plant science, clinical diagnosis, catalyst studies, and materials science.
authors: Jiangxi Key Laboratory for Mass Spectrometry and Instrumentation, East China Institute of Technology, Nanchang, Jiangxi 330013, China.
authors: Hua
authors: H
authors: Zhang
authors: Haiwei
authors: H
authors: Gu
authors: Feiyan
authors: F
authors: Yan
authors: Nannan
authors: N
authors: Wang
authors: Yiping
authors: Y
authors: Wei
authors: Jianjun
authors: J
authors: Xu
authors: Huanwen
authors: H
authors: Chen
mesh_terms: D001704:Biopolymers / Q000032:analysis*; D045424:Complex Mixtures / Q000032:analysis*; D018401:Sample Size; D052616:Solid Phase Extraction / Q000379:methods*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
chemical_list: D001704:Biopolymers; D045424:Complex Mixtures
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