title: Development of a loop-mediated isothermal amplification assay targeting the mpb64 gene for diagnosis of intraocular tuberculosis.
abstract: A loop-mediated isothermal amplification (LAMP) assay targeting the mpb64 gene for the diagnosis of intraocular tuberculosis was highly specific (100%), sensitive (85.7%), rapid, and easy to perform. The LAMP assay can be an alternative to conventional PCR for the diagnosis of ocular tuberculosis in resource-limited settings.
authors: Ocular Microbiology Service.
authors: Praveen Kumar
authors: PK
authors: Balne
authors: Manas Ranjan
authors: MR
authors: Barik
authors: Savitri
authors: S
authors: Sharma
authors: Soumyava
authors: S
authors: Basu
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics; D001431:Bacteriological Techniques / Q000379:methods*; D006801:Humans; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D009169:Mycobacterium tuberculosis / Q000235:genetics / Q000302:isolation & purification*; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D012680:Sensitivity and Specificity; D013997:Time Factors; D014392:Tuberculosis, Ocular / Q000175:diagnosis* / Q000382:microbiology*
chemical_list: D001426:Bacterial Proteins
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