title: Qidantongmai protects endothelial cells against hypoxia-induced damage through regulating the serum VEGF-a level.
abstract: Qidantongmai (QDTM) is a Traditional Chinese Medicine (TCM) preparation that has long been used in folk medicine for the treatment of cardiovascular diseases. However, the underlying mechanisms are poorly understood. The present study was designed to determine the effects of QDTM on endothelial cells under hypoxic conditions both in vitro and in vivo. Primary human umbilical vein endothelial cells (HUVECs) were isolated, pretreated with QDTM medicated serum or saline control, and then cultured under hypoxia (2% oxygen) for 24 h. Sprague-Dawley rats were administered 1 ml/100 g of QDTM or saline twice a day for 4 days and treated with hypoxia (6 hours/day, discontinuous hypoxia, 360 mm Hg). QDTM not only protected HUVECs from hypoxia-induced damage by significantly retaining cell viability (P < 0.05) and decreasing apoptosis (P < 0.05) in vitro, but also protected liver endothelial cells from hypoxia-induced damage in vivo. Moreover, QDTM increased the serum VEGF-A level (P < 0.05) in rats treated with hypoxia for 7 days but suppressed the upregulation of serum VEGF-A in rats treated with hypoxia for 14 days. QDTM is a potent preparation that can protect endothelial cells against hypoxia-induced damage. The ability of QDTM to modulate the serum VEGF-A level may play an important role in its effects on endothelial cells.
authors: Department of Traditional Chinese Medicine, Xijing Hospital, The Fourth Military Medical University, Xi'an, Shanxi 710032, People's Republic of China.
authors: Bing
authors: B
authors: Wang
authors: Wen
authors: W
authors: Wang
authors: Feng
authors: F
authors: Li
authors: Zongren
authors: Z
authors: Wang
authors: Jing
authors: J
authors: Ma
authors: Gang
authors: G
authors: Zhao
mesh_terms: D029971:Angelica sinensis; D000818:Animals; D027883:Astragalus Plant; D031187:Carthamus tinctorius; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D042783:Endothelial Cells / Q000187:drug effects* / Q000473:pathology; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects* / Q000473:pathology; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D000860:Hypoxia; D008099:Liver / Q000166:cytology / Q000187:drug effects* / Q000473:pathology; D019684:Magnoliopsida; D008297:Male; D008517:Phytotherapy; D051381:Rats; D017207:Rats, Sprague-Dawley; D027544:Salvia; D042461:Vascular Endothelial Growth Factor A / Q000097:blood*
chemical_list: D004365:Drugs, Chinese Herbal; D042461:Vascular Endothelial Growth Factor A
keywords: Traditional Chinese Medicine; VEGF; human umbilical vein endothelial cells; hypoxia
references: Biochem Biophys Res Commun. 2009 Dec 18;390(3):845-8
references: 19836352
references: Cardiovasc Res. 2005 Feb 15;65(3):550-63
references: 15664381
references: Ann Neurol. 2010 Jun;67(6):802-8
references: 20517942
references: Ann Neurol. 2010 Jul;68(1):70-80
references: 20582986
references: Curr Opin Crit Care. 2002 Jun;8(3):242-50
references: 12386504
references: Ann Neurol. 2011 Feb;69(2):360-74
references: 21246601
references: Ann Neurol. 2010 Aug;68(2):184-92
references: 20695011
references: Mol Med. 2011 May-Jun;17(5-6):523-32
references: 21267512
references: Ann Neurol. 2010 Jul;68(1):58-69
references: 20582984
references: Zhongguo Zhong Xi Yi Jie He Za Zhi. 2001 Nov;21(11):825-7
references: 12575375
references: Cardiovasc Res. 2008 May 1;78(2):356-65
references: 18093986
references: Nat Rev Cancer. 2008 Aug;8(8):579-91
references: 18596824
references: Am J Respir Cell Mol Biol. 2000 Jun;22(6):657-64
references: 10837361
references: Intern Med. 2000 Dec;39(12):1101-4
references: 11197800
references: J Mol Cell Cardiol. 2007 Jun;42(6):1036-44
references: 17498737
references: Ann Neurol. 2010 Dec;68(6):835-44
references: 21194153
references: Vascul Pharmacol. 2009 Mar-Apr;50(3-4):137-45
references: 19084079
references: Biochem Biophys Res Commun. 2002 May 10;293(3):979-85
references: 12051756
references: Biomed Mater Eng. 2008;18(4-5):295-9
references: 19065037
references: Ann Neurol. 2010 Apr;67(4):488-97
references: 20437584
references: Circulation. 1996 Dec 15;94(12):3062-4
references: 8989106
references: Cardiovasc Res. 2008 May 1;78(2):333-40
references: 18006432
references: ILAR J. 2009;50(3):282-8
references: 19506314
references: Biol Pharm Bull. 2005 Aug;28(8):1450-4
references: 16079491
references: J Cardiovasc Pharmacol. 2008 Aug;52(2):191-202
references: 18670359
references: Am J Pathol. 2007 Apr;170(4):1348-61
references: 17392173
references: Cardiovasc Res. 2008 May 1;78(2):315-23
references: 18065770
references: Nat Med. 2003 Jun;9(6):669-76
references: 12778165
references: Ann Neurol. 2010 Sep;68(3):330-41
references: 20658563
references: Adv Exp Med Biol. 2007;618:69-84
references: 18269189
references: Autophagy. 2012 Jan;8(1):77-87
references: 22113203
references: Life Sci. 2004 Aug 20;75(14):1775-86
references: 15268976
references: Vascul Pharmacol. 2007 Apr;46(4):278-85
references: 17196887