title: Pectinase hydrolysis of Dendrobium huoshanense polysaccharide and its effect on protein nonenzymatic glycation.
abstract: The aim of this study was to investigate the inhibitory effects of molecular weight alteration of Dendrobium huoshanense polysaccharide on protein nonenzymatic glycation. For this purpose, one homogeneous active polysaccharide DHPD1 with molecular weight 3.2 kDa was extracted from D. huoshanense. GC analysis showed that DHPD1 was mainly composed of glucose, arabinose, galactose in a molar ratio of 0.023:1.023:0.021 with a trace of mannose and xylose. In order to get DHPD1-derived fragments with different molecular weight, response surface methodology was employed to optimize the enzymatic degradation conditions. The maximum reducing sugar production (0.399 mg/mL) was obtained under an optimal condition including pectinase dosage 126 U/mL, reaction pH 4.46 and reaction temperature 48 °C. By applying this condition, three DHPD1-derived fragments with different molecular weights were obtained through changing the hydrolysis time. Infrared spectroscopy analysis indicated that the backbone structure of DHPD1 was not destroyed by pectinase hydrolysis. Monosaccharide composition analysis showed that pectinase preferred to liberate glucose from DHPD1. The inhibitory action of DHPD1 on protein nonenzymatic glycation reduced with the decrease of molecular weight.
authors: School of Biotechnology and Food Engineering, Hefei University of Technology, No. 193 Tunxi Road, Hefei 230009, People's Republic of China. Electronic address: zhaxueqiang@hfut.edu.cn.
authors: Xue-Qiang
authors: XQ
authors: Zha
authors: Xiao-Long
authors: XL
authors: Li
authors: Hai-Lin
authors: HL
authors: Zhang
authors: Shao-Hua
authors: SH
authors: Cui
authors: Jian
authors: J
authors: Liu
authors: Jun-Hui
authors: JH
authors: Wang
authors: Li-Hua
authors: LH
authors: Pan
authors: Jian-Ping
authors: JP
authors: Luo
mesh_terms: D031667:Dendrobium / Q000737:chemistry* / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007700:Kinetics; D008956:Models, Chemical; D008970:Molecular Weight; D009005:Monosaccharides / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry; D010940:Plant Proteins / Q000737:chemistry / Q000378:metabolism*; D011096:Polygalacturonase / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry*; D059748:Proteolysis; D015203:Reproducibility of Results; D017550:Spectroscopy, Fourier Transform Infrared; D013696:Temperature
chemical_list: D009005:Monosaccharides; D010936:Plant Extracts; D010940:Plant Proteins; D011134:Polysaccharides; D011096:Polygalacturonase
keywords: Dendrobium huoshanense polysaccharide; Enzymatic degradation; Nonenzymatic glycation of proteins