title: Mutation scanning analysis of genetic variation within and among Echinococcus species: implications and future prospects.
abstract: Adult tapeworms of the genus Echinococcus (family Taeniidae) occur in the small intestines of carnivorous definitive hosts and are transmitted to particular intermediate mammalian hosts, in which they develop as fluid-filled larvae (cysts) in internal organs (usually lung and liver), causing the disease echinococcosis. Echinococcus species are of major medical importance and also cause losses to the meat and livestock industries, mainly due to the condemnation of infected offal. Decisions regarding the treatment and control of echinococcosis rely on the accurate identification of species and population variants (strains). Conventional, phenetic methods for specific identification have some significant limitations. Despite advances in the development of molecular tools, there has been limited application of mutation scanning methods to species of Echinococcus. Here, we briefly review key genetic markers used for the identification of Echinococcus species and techniques for the analysis of genetic variation within and among populations, and the diagnosis of echinococcosis. We also discuss the benefits of utilizing mutation scanning approaches to elucidate the population genetics and epidemiology of Echinococcus species. These benefits are likely to become more evident following the complete characterization of the genomes of E. granulosus and E. multilocularis.
authors: Faculty of Veterinary Science, The University of Melbourne, Parkville, Victoria, Australia.
authors: Abdul
authors: A
authors: Jabbar
authors: Robin B
authors: RB
authors: Gasser
mesh_terms: D000818:Animals; D004252:DNA Mutational Analysis; D018509:DNA, Helminth / Q000032:analysis / Q000737:chemistry / Q000235:genetics; D004443:Echinococcosis / Q000469:parasitology*; D004446:Echinococcus / Q000235:genetics* / Q000254:growth & development / Q000302:isolation & purification; D005819:Genetic Markers / Q000235:genetics; D049751:Genome, Helminth / Q000235:genetics; D006801:Humans; D025202:Molecular Diagnostic Techniques
chemical_list: D018509:DNA, Helminth; D005819:Genetic Markers