title: Ixodes ricinus abundance and its infection with the tick-borne pathogens in urban and suburban areas of Eastern Slovakia.
abstract: BACKGROUND
  Raising abundance of ticks and tick-borne diseases in Europe is the result of multiple factors including climate changes and human activities. Herein, we investigated the presence and seasonal activity of Ixodes ricinus ticks from 10 urban and suburban sites in two different geographical areas of southeastern and northeastern Slovakia during 2008-2010. Our aim was to study the abundance of ticks in correlation with the environmental factors and their infection with Borrelia burgdorferi sensu lato, Anaplasma phagocytophilum and Neoehrlichia mikurensis.
  
  
  METHODS
  Questing I. ricinus ticks were collected from ten urban and suburban sites in Eastern Slovakia. A total of 670 ticks were further analysed for the presence of B. burgdorferi s.l., A. phagocytophilum and N. mikurensis by molecular methods. Tick site and environmental relations were analysed using General Linear Models (LM). The differences between the number of Lyme borreliosis cases between the Košice and Bardejov regions during a ten-year period were tested by Wilcoxon matched pairs test.
  
  
  RESULTS
  In total, 2921 (1913 nymphs, 1008 adults) I. ricinus ticks were collected from 10 study sites during the main questing season. Tick activity and relative abundance differed between locations and months. Temperature and humidity were the main factors affecting the tick abundance and questing activity. Out of 670 examined ticks, 10.15% were infected with spirochetes from B. burgdorferi s.l. complex (represented by B. afzelii, B. garinii, B.valaisiana and B. burgdorferi s.s.), 2.69% with the A. phagocytophilum and 2.39% with N. mikurensis. The number of Lyme borreliosis cases per 100,000 inhabitants in the Bardejov region was significantly higher than in the Košice region.
  
  
  CONCLUSIONS
  Our data indicate that the risk of infection with tick-borne pathogens in Eastern Slovakia is common since 15.2% of ticks were infected at least with one of the tested microorganisms. Even though the abundance of ticks was affected by the microclimatic conditions and the prevalence of pathogens differed between the habitats, the infection risk for humans is also affected by human activities leading to an increased contact with infected ticks.
authors: Institute of Parasitology SAS, Košice, Hlinkova, Slovakia.
authors: Lucia
authors: L
authors: Pangrácová
authors: Markéta
authors: M
authors: Derdáková
authors: Ladislav
authors: L
authors: Pekárik
authors: Ivana
authors: I
authors: Hviščová
authors: Bronislava
authors: B
authors: Víchová
authors: Michal
authors: M
authors: Stanko
authors: Helena
authors: H
authors: Hlavatá
authors: Branislav
authors: B
authors: Peťko
mesh_terms: D041081:Anaplasma phagocytophilum / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D000710:Anaplasmataceae / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D000818:Animals; D001096:Arachnid Vectors / Q000382:microbiology*; D025065:Borrelia burgdorferi / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D006801:Humans; D018884:Ixodes / Q000382:microbiology*; D018154:Slovakia; D018881:Suburban Health; D017282:Tick-Borne Diseases / Q000453:epidemiology* / Q000382:microbiology; D014504:Urban Health
references: J Clin Microbiol. 2010 May;48(5):1956-9
references: 20220155
references: Neuro Endocrinol Lett. 2011;32(4):491-5
references: 21876490
references: Appl Environ Microbiol. 2012 Jul;78(13):4606-12
references: 22522688
references: Ann Agric Environ Med. 2010;17(1):173-5
references: 20684497
references: J Med Entomol. 1999 Nov;36(6):741-8
references: 10593075
references: Int J Syst Evol Microbiol. 2004 Sep;54(Pt 5):1837-1843
references: 15388752
references: Parasit Vectors. 2013 Jan 02;6:1
references: 23281838
references: J Clin Microbiol. 2010 Jul;48(7):2630-5
references: 20519481
references: Diagn Microbiol Infect Dis. 2011 Mar;69(3):266-70
references: 21353949
references: Parasitol Res. 2000 Jul;86(7):554-7
references: 10935905
references: Vet Parasitol. 2010 Feb 10;167(2-4):149-54
references: 19836890
references: Curr Probl Dermatol. 2009;37:31-50
references: 19367096
references: J Clin Microbiol. 2005 Mar;43(3):1309-17
references: 15750101
references: Emerg Infect Dis. 2010 Jul;16(7):1127-9
references: 20587186
references: Euro Surveill. 2010 Jul 08;15(27):24-31
references: 20630144
references: Vet Parasitol. 2011 Dec 29;183(1-2):114-24
references: 21820245
references: Ann Agric Environ Med. 2006;13(2):301-6
references: 17196005
references: Wien Klin Wochenschr. 2006 Dec;118(23-24):759-64
references: 17186172
references: Infect Genet Evol. 2011 Oct;11(7):1545-63
references: 21843658
references: Ticks Tick Borne Dis. 2012 Apr;3(2):90-4
references: 22445929
references: PLoS One. 2012;7(9):e45511
references: 23029063
references: Parasit Vectors. 2013 Jun 20;6:187
references: 23786850
references: Vet Microbiol. 2011 Dec 15;153(3-4):293-8
references: 21684091
references: Int J Med Microbiol. 2006 May;296 Suppl 40:108-18
references: 16530478
references: Parasit Vectors. 2012 Jan 10;5:8
references: 22233771
references: J Clin Pathol. 1991 Jul;44(7):610-1
references: 1856296
references: J Clin Microbiol. 1999 Jul;37(7):2215-22
references: 10364588
references: Emerg Infect Dis. 2012 Dec;18(12):2063-5
references: 23171720
references: Appl Environ Microbiol. 2003 Jan;69(1):509-16
references: 12514035
references: Parasit Vectors. 2012 Apr 19;5:74
references: 22515314
references: Emerg Infect Dis. 2010 Mar;16(3):524-6
references: 20202437
references: Ann Agric Environ Med. 2003;10(2):269-71
references: 14677924
references: Nucleic Acids Res. 1997 Sep 1;25(17):3389-402
references: 9254694
references: Vet Parasitol. 2009 Nov 12;165(3-4):357-60
references: 19682799
references: Clin Infect Dis. 1993 Oct;17(4):708-17
references: 7903558
references: Parasite. 2008 Sep;15(3):244-7
references: 18814688
references: Cent Eur J Public Health. 2005 Mar;13(1):24-8
references: 15859176
references: J Clin Microbiol. 2011 May;49(5):2059-62
references: 21367991
references: Parasit Vectors. 2013 Jul 09;6:202
references: 23837798
references: Parasit Vectors. 2012 Jul 25;5:149
references: 22830528
references: Vet Parasitol. 2009 Oct 14;164(2-4):275-81
references: 19560275
references: Vet Parasitol. 2010 Feb 10;167(2-4):108-22
references: 19811878