title: Fucoidans as potential inhibitors of HIV-1.
abstract: The antiviral activity of different structure fucoidans (α-l-fucans and galactofucans) was studied using two model viral systems based on a lentiviral vectors and a replication competent Moloney murine leukemia virus (Mo-MuLV). It was found that investigated fucoidans have no cytotoxic effects on Jurkat and SC-1cell at the concentration range of 0.001-100 µg/mL. Fucoidans with different efficiency suppressed transduction of Jurkat cell line by pseudo-HIV-1 particles carrying the envelope protein of HIV-1 and infection of SC-1 cells by Mo-MuLV. According to our data, all natural fucoidans can be considered as potential anti-HIV agents regardless of their carbohydrate backbone and degree of sulfating, since their activity is shown at low concentrations (0.001-0.05 µg/mL). High molecular weight fucoidans isolated from Saccharina cichorioides (1.3-α-l-fucan), and S. japonica (galactofucan) were the most effective inhibitors.
authors: Laboratory of Cell Biology, Engelhardt-Institute of Molecular Biology, Moscow 119991, Russia. m.prokofjeva@gmail.com
authors: Maria M
authors: MM
authors: Prokofjeva
authors: Tatyana I
authors: TI
authors: Imbs
authors: Natalya M
authors: NM
authors: Shevchenko
authors: Pavel V
authors: PV
authors: Spirin
authors: Stefan
authors: S
authors: Horn
authors: Boris
authors: B
authors: Fehse
authors: Tatyana N
authors: TN
authors: Zvyagintseva
authors: Vladimir S
authors: VS
authors: Prassolov
mesh_terms: D019380:Anti-HIV Agents / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D000998:Antiviral Agents / Q000008:administration & dosage / Q000494:pharmacology; D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D005822:Genetic Vectors; D015497:HIV-1 / Q000187:drug effects*; D006801:Humans; D066298:In Vitro Techniques; D019169:Jurkat Cells; D016086:Lentivirus / Q000235:genetics; D008970:Molecular Weight; D008979:Moloney murine leukemia virus / Q000187:drug effects*; D000459:Phaeophyceae / Q000737:chemistry; D011134:Polysaccharides / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D019380:Anti-HIV Agents; D000998:Antiviral Agents; D011134:Polysaccharides; C007789:fucoidan
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