title: Panton-Valentine leukocidin producing Staphylococcus aureus nasal carriage, in North-Lebanon.
abstract: OBJECTIVE
  We investigated the prevalence and the risk factors of nasal carriage of Staphylococcus aureus carrying Panton-Valentine leukocidin genes [Sa PVL(+)] in pupils.
  
  
  METHODS
  Two hundred and fifty-seven pupils were screened by nasal swabbing. The detection of 16S rRNA, mecA and luk-PV genes was performed by PCR and the risk factors were assessed with the statistical analysis of a questionnaire.
  
  
  RESULTS
  Thirty-one percent of pupils were colonized, with 16.4% of isolates carrying the luk-PV gene and 8.8% the mecA gene. Children aged 7years or more and living in a boarding school were the factors promoting nasal carriage 60% of children who presented with an abscess in the previous year were carriers of luk-PV gene Sa.
  
  
  CONCLUSIONS
  The study revealed a high prevalence of luk-PV gene among methicillin-susceptible strains and a statistically significant correlation between the presence of this gene and presenting with an abscess.
authors: Laboratoire de microbiologie santé et environnement, centre AZM pour la recherche en biotechnologies et ses applications, école doctorale des sciences et de technologie, université Libanaise, Tripoli, Lebanon.
authors: R
authors: R
authors: Beyrouthy
authors: M
authors: M
authors: Hamze
authors: S
authors: S
authors: Hleis
authors: H
authors: H
authors: Mallat
authors: F
authors: F
authors: Dabboussi
mesh_terms: D000293:Adolescent; D001426:Bacterial Proteins / Q000235:genetics; D001427:Bacterial Toxins / Q000032:analysis* / Q000235:genetics; D002353:Carrier State / Q000453:epidemiology* / Q000382:microbiology; D002648:Child; D002675:Child, Preschool; D005098:Exotoxins / Q000032:analysis* / Q000235:genetics; D005260:Female; D005667:Furunculosis / Q000382:microbiology; D005798:Genes, Bacterial; D006798:Housing; D006801:Humans; D007861:Lebanon / Q000453:epidemiology; D007956:Leukocidins / Q000032:analysis* / Q000235:genetics; D008297:Male; D008712:Methicillin / Q000494:pharmacology; D009296:Nasal Cavity / Q000382:microbiology*; D046915:Penicillin-Binding Proteins; D015995:Prevalence; D021521:Ribotyping; D012574:Schools; D013203:Staphylococcal Infections / Q000453:epidemiology* / Q000382:microbiology; D013211:Staphylococcus aureus / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification*; D014774:Virulence
chemical_list: D001426:Bacterial Proteins; D001427:Bacterial Toxins; D005098:Exotoxins; D007956:Leukocidins; C078284:Panton-Valentine leukocidin; D046915:Penicillin-Binding Proteins; C487789:mecA protein, Staphylococcus aureus; D008712:Methicillin
keywords: Leucocidine de Panton-Valentine; Nasal carriage; Panton-Valentine leukocidin; Portage nasal; S. aureus