title: Unaltered lactate and glucose transporter levels in the MPTP mouse model of Parkinson's disease.
abstract: BACKGROUND
  Metabolic impairment contributes to development of Parkinson's disease (PD). Mitochondrial dysfunction is involved in degeneration of nigral dopamine neurons. Also, in PD there are alterations in glucose metabolism in nigro-striatal pathways, and increased cerebral lactate levels have been found.
  
  
  OBJECTIVE
  We raise the question of whether changes in the amount transporters of energy substrates are involved in the pathogenesis of PD.
  
  
  METHODS
  We have used confocal immunofluorescence and immunogold postembedding electron microscopic techniques to study whether there are altered levels of the transporters for monocarboxylates (MCT1 and MCT2) and glucose (GLUT1) in the MPTP mouse model of PD.
  
  
  RESULTS
  We found that MCT1 and GLUT1 were densely located in blood vessel endothelium, while MCT2 was present in perivascular astrocytic end feet processes in the substantia nigra and the striatum of control mice. We found that the localisation and densities of MCTs and GLUT1 were unaltered in the PD model.
  
  
  CONCLUSIONS
  This is the first study reporting on the distribution of metabolic transporters in PD. Our results suggest that, although there are metabolic impairments in PD, the levels of MCT1, MCT2 and GLUT1 is not changed following dopaminergic neurodegeneration. This is in contrast to findings in other neurodegenerative disease, such as mesial temporal lobe epilepsy, where there are large alterations in MCT levels.
authors: Glio- and Neurotransmitter Group, Centre for Molecular Biology and Neuroscience and Institute for Basic Medical Sciences, University of Oslo, Oslo, Norway.
authors: Maja
authors: M
authors: Puchades
authors: Carl Johan
authors: CJ
authors: Sogn
authors: Jan
authors: J
authors: Maehlen
authors: Linda H
authors: LH
authors: Bergersen
authors: Vidar
authors: V
authors: Gundersen
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000378:metabolism; D001923:Brain Chemistry / Q000502:physiology; D005455:Fluorescent Antibody Technique; D051246:Glucose Transport Proteins, Facilitative / Q000378:metabolism*; D051272:Glucose Transporter Type 1 / Q000096:biosynthesis / Q000235:genetics; D006801:Humans; D007150:Immunohistochemistry; D020267:MPTP Poisoning / Q000378:metabolism*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018613:Microscopy, Confocal; D008854:Microscopy, Electron; D027501:Monocarboxylic Acid Transporters / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D017072:Neostriatum / Q000378:metabolism; D010300:Parkinson Disease / Q000378:metabolism*; D013378:Substantia Nigra / Q000378:metabolism; D027981:Symporters / Q000096:biosynthesis / Q000235:genetics; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism; D000099233:Monocarboxylate Transport Protein 1
chemical_list: D051246:Glucose Transport Proteins, Facilitative; D051272:Glucose Transporter Type 1; D027501:Monocarboxylic Acid Transporters; D027981:Symporters; D014446:Tyrosine 3-Monooxygenase; D000099233:Monocarboxylate Transport Protein 1; C490426:Slc16a7 protein, mouse; C495678:Slc2a1 protein, mouse