title: Comparison of matrix-assisted laser desorption ionization-time of flight (maldi-tof) mass spectrometry platforms for the identification of gram-negative rods from patients with cystic fibrosis.
abstract: We evaluated the performance of the Bruker Biotyper and the bioMérieux Vitek MS with both the SARAMIS v4.09 and Knowledge Base v2.0 databases for the identification of 203 non-glucose-fermenting Gram-negative rods that had previously been identified by 16S rRNA gene sequencing. Including those that underwent repeat testing, 96.6%, 90.1%, and 93.6% of isolates, respectively, had identifications that agreed with the previous identification.
authors: Clinical Microbiology/Immunology Laboratories, UNC Health Care, Chapel Hill, North Carolina, USA.
authors: Kevin
authors: K
authors: Alby
authors: Peter H
authors: PH
authors: Gilligan
authors: Melissa B
authors: MB
authors: Miller
mesh_terms: D001431:Bacteriological Techniques / Q000379:methods*; D003550:Cystic Fibrosis / Q000150:complications*; D006090:Gram-Negative Bacteria / Q000737:chemistry* / Q000145:classification* / Q000302:isolation & purification; D016905:Gram-Negative Bacterial Infections / Q000382:microbiology*; D006801:Humans; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
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