title: Harpagophytum procumbens prevents oxidative stress and loss of cell viability in vitro.
abstract: Harpagophytum procumbens, popularly known as devil's claw, is a plant commonly used in the treatment of diseases of inflammatory origin. The anti-inflammatory effects of H. procumbens have been studied; however, the mechanism of action is not elucidated. It is known that excess of reactive oxygen and nitrogen species may contribute to increasing tissue damage due to inflammation. In the present study, we examined the effects of H. procumbens infusion, crude extract and fractions on lipid peroxidation (brain homogenates) induced by different pro-oxidants (Fe(2+) or sodium nitroprusside) and the effects of ethyl acetate fraction (rich in phenolic compounds) on antioxidant defenses (catalase activity and thiol levels) and cell damage (brain cortical slices) induced by different pro-oxidants. All tested extracts of H. procumbens inhibited lipid peroxidation in a concentration-dependent manner. Furthermore, the ethyl acetate fraction had the highest antioxidant effects either by decreasing lipid peroxidation and cellular damage or restoring thiols levels and catalase activity. Taken together, our results showed that H. procumbens acts either by preventing oxidative stress or loss of cell viability. Thus, the previously reported anti-inflammatory effect of H. procumbens could also be attributed to its antioxidant activity.
authors: Programa de Pós-Graduação em Farmacologia, Universidade Federal de Santa Maria, Santa Maria, RS, 97105-900, Brazil, lari_biomedica@yahoo.com.br.
authors: Larissa Finger
authors: LF
authors: Schaffer
authors: Luis Ricardo
authors: LR
authors: Peroza
authors: Aline Augusti
authors: AA
authors: Boligon
authors: Margareth Linde
authors: ML
authors: Athayde
authors: Sydney Hartz
authors: SH
authors: Alves
authors: Roselei
authors: R
authors: Fachinetto
authors: Caroline
authors: C
authors: Wagner
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D002540:Cerebral Cortex / Q000187:drug effects / Q000378:metabolism; D031684:Harpagophytum / Q000737:chemistry*; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects*; D010936:Plant Extracts / Q000494:pharmacology*
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D000975:Antioxidants; D010936:Plant Extracts
references: Arch Neurol. 1991 Dec;48(12):1285-93
references: 1845035
references: J Mol Med (Berl). 2013 Mar;91(3):323-8
references: 23430240
references: Oxid Med Cell Longev. 2013;2013:690545
references: 23710287
references: Arch Biochem Biophys. 2008 Dec 1;480(1):27-32
references: 18835240
references: Exp Toxicol Pathol. 2011 Mar;63(3):249-55
references: 20122821
references: Phytother Res. 2004 Dec;18(12):982-9
references: 15742343
references: Neurochem Res. 2009 Aug;34(8):1372-9
references: 19191025
references: Bull World Health Organ. 1985;63(6):965-81
references: 3879679
references: Planta Med. 2003 Aug;69(8):720-4
references: 14531022
references: Phytother Res. 2012 Feb;26(2):313-6
references: 21721061
references: Biochim Biophys Acta. 2013 Jun;1830(6):3670-95
references: 23428572
references: Pharmacol Biochem Behav. 2012 Apr;101(2):307-10
references: 22266770
references: Bioresour Technol. 2009 Dec;100(24):6592-8
references: 19666219
references: Arch Biochem Biophys. 1959 May;82(1):70-7
references: 13650640
references: Biochim Biophys Acta. 2009 Jul;1790(7):629-36
references: 18948172
references: Biochem Pharmacol. 1991 Dec 11;42 Suppl:S157-65
references: 1768273
references: J Neurochem. 1992 Nov;59(5):1609-23
references: 1402908
references: Neurochem Res. 2013 Apr;38(4):789-96
references: 23377855
references: Nature. 2000 Nov 9;408(6809):239-47
references: 11089981
references: Proc Natl Acad Sci U S A. 1990 Feb;87(4):1620-4
references: 2154753
references: J Physiol Pharmacol. 2003 Dec;54(4):469-87
references: 14726604
references: Neurochem Int. 2013 Apr;62(5):764-75
references: 23422879
references: J Rheumatol. 2013 Jun;40(6):943-8
references: 23547210
references: J Ethnopharmacol. 2004 Apr;91(2-3):325-30
references: 15120457
references: Pharmacol Biochem Behav. 2013 Mar;104:132-7
references: 23313549
references: J Smooth Muscle Res. 2009 Oct;45(5):231-9
references: 19907121
references: J Appl Toxicol. 2005 Jan-Feb;25(1):82-8
references: 15669027
references: Phytother Res. 2010 Sep;24(9):1365-9
references: 20812280
references: Pharmacol Ther. 1992;53(3):375-408
references: 1409852
references: Phytomedicine. 2004 Nov;11(7-8):585-95
references: 15636171
references: Anal Biochem. 1979 Jun;95(2):351-8
references: 36810
references: Biochem J. 1984 Dec 15;224(3):761-7
references: 6098266
references: Phytother Res. 2009 Jan;23(1):104-10
references: 18803229
references: Neurochem Int. 2013 Apr;62(5):575-94
references: 23266600
references: Phytother Res. 2007 Mar;21(3):199-209
references: 17128436
references: Oxid Med Cell Longev. 2009 Nov-Dec;2(5):290-6
references: 20716916
references: Food Chem. 2011 Jun 15;126(4):1850-5
references: 25213967
references: Oxid Med Cell Longev. 2012;2012:537923
references: 22900128
references: Oxid Med Cell Longev. 2012;2012:157598
references: 23320126
references: Phytother Res. 2007 Dec;21(12):1228-33
references: 17886223
references: Bioresour Technol. 2009 Jan;100(1):509-14
references: 18280147
references: Brain Res Bull. 2006 Mar 15;69(1):57-62
references: 16464685
references: Curr Opin Chem Biol. 1999 Apr;3(2):200-6
references: 10226041
references: Free Radic Biol Med. 2010 Dec 1;49(11):1603-16
references: 20840865
references: Med Biol. 1984;62(2):71-7
references: 6088908
references: Free Radic Res. 2013 May;47(5):346-56
references: 23438723
references: Neurochem Int. 2013 Jun;62(8):1039-47
references: 23562769
references: Free Radic Biol Med. 1998 May;24(7-8):1065-73
references: 9626559
references: Neurochem Res. 2009 May;34(5):973-83
references: 18853256
references: J Neurochem. 1998 Jul;71(1):69-77
references: 9648852
references: Biol Pharm Bull. 2008 Feb;31(2):240-5
references: 18239280
references: Pharmazie. 2007 Nov;62(11):876-80
references: 18065107
references: Brain Res. 2006 Aug 30;1107(1):192-8
references: 16828712
references: Drug Discov Ther. 2010 Jun;4(3):144-67
references: 22491178
references: Neurotoxicology. 2008 Jul;29(4):727-34
references: 18541302
references: Am J Med. 2000 Jul;109(1):33-44
references: 10936476
references: Neuroreport. 1991 Mar;2(3):121-3
references: 1662993
references: J Nat Prod. 2008 May;71(5):746-9
references: 18412394
references: Methods Enzymol. 1984;105:121-6
references: 6727660
references: J Ethnopharmacol. 2008 Oct 28;119(3):342-55
references: 18577439
references: Proc Natl Acad Sci U S A. 1991 Jul 15;88(14):6368-71
references: 1648740
references: J Biol Chem. 1951 Nov;193(1):265-75
references: 14907713
references: Phytother Res. 2001 Nov;15(7):621-4
references: 11746846
references: J Altern Complement Med. 2006 Dec;12(10):981-93
references: 17212570
references: J Ethnopharmacol. 2012 Oct 11;143(3):755-71
references: 22940241
references: Phytother Res. 2012 Jun;26(6):806-11
references: 22072539