title: Anti-tumor activity of the regenerated triple-helical polysaccharide from Dictyophora indusiata.
abstract: A triple helical polysaccharide (PD3) was isolated from Dictyophora indusiata. After denaturation in dilute NaOH solution (0.3 M) and renaturation by sequential dialysis, regenerated polysaccharide (RPD3) was obtained. The physico-chemical properties of RPD3 including intrinsic viscosity [η], molecular weight (Mw) and optical rotation were similar to those of PD3, which suggested that RPD3 also had a triple helical structure after denaturation-renaturation. However, different intrinsic viscosity dependence on the concentration of NaOH solution was noted in PD3 and RPD3, which indicated that RPD3 had lower chain tightness compared with PD3. The anti-tumor activity of this polysaccharide after denaturation-renaturation treatment was further investigated. Both PD3 and RPD3 showed no direct cytotoxicity against S-180 cells in vitro but behaved anti-tumor activity in vivo. Meanwhile, RPD3 in high-dose group showed much higher anti-tumor activity than that of PD3, suggesting that the denaturation-renaturation treatment improved the bioactivity of the polysaccharide from D. indusiata.
authors: Wuxi Medical School, Jiangnan University, Wuxi 214122, PR China; School of Pharmaceutical Science, Jiangnan University, Wuxi 214122, PR China.
authors: Chao
authors: C
authors: Deng
authors: Haitian
authors: H
authors: Fu
authors: Liping
authors: L
authors: Teng
authors: Zhun
authors: Z
authors: Hu
authors: Xiaofei
authors: X
authors: Xu
authors: Jinghua
authors: J
authors: Chen
authors: Tianli
authors: T
authors: Ren
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry* / Q000494:pharmacology* / Q000633:toxicity; D001487:Basidiomycota / Q000737:chemistry*; D001835:Body Weight / Q000187:drug effects; D045744:Cell Line, Tumor; D016207:Cytokines / Q000097:blood / Q000378:metabolism; D062610:Fungal Polysaccharides / Q000737:chemistry* / Q000494:pharmacology* / Q000633:toxicity; D008297:Male; D051379:Mice; D008970:Molecular Weight; D009369:Neoplasms / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D014783:Viscosity
chemical_list: D000970:Antineoplastic Agents; D016207:Cytokines; D062610:Fungal Polysaccharides
keywords: Anti-tumor activity; Denaturation–renaturation; Dictyophora indusiata polysaccharide