title: Analysis of DNA repair helicase UvrD from Arabidopsis thaliana and Oryza sativa.
abstract: Mismatch repair (MMR) proteins play important roles in maintaining genome stability in all the organisms. Studies of MMR genes in plants have identified several homologs of the Escherichia coli genes. Crop yield is directly related to genome stability, which is crucially required for optimal plant growth and development. Numerous genotoxic stresses such as UV light, radiations, pollutants and heavy metals cause DNA damage leading to genome instability, which can interfere with the plant growth and crop productivity. But the efficient repair mechanisms can help to overcome the deleterious effects of the damage. Therefore it is important to study the genes involved in various repair pathways in the plants in greater detail. UvrD helicase is a component of MMR complex and plays an essential role in the DNA repair by providing the unwinding function. In the present manuscript we present an in silico analysis of UvrD helicase from two plant species (Arabidopsis and rice). The Arabidopsis thaliana and Oryza sativa UvrD are 1149 (~129 kDa) and 1165 amino-acids (~130 kDa) proteins, respectively. These proteins contain all the conserved domains and are larger than the E. coli UvrD because they contain a longer N-terminal extension. In order to decipher the role of plant UvrD in various stresses it will be important to study the biochemical and functional properties of this enzyme.
authors: International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi 110067, India. Electronic address: renu@icgeb.res.in.
authors: Renu
authors: R
authors: Tuteja
authors: Narendra
authors: N
authors: Tuteja
mesh_terms: D017360:Arabidopsis / Q000201:enzymology*; D004265:DNA Helicases / Q000378:metabolism*; D012275:Oryza / Q000201:enzymology*; D010940:Plant Proteins / Q000378:metabolism*
chemical_list: D010940:Plant Proteins; D004265:DNA Helicases
keywords: Arabidopsis; BER; DNA repair; DR; E. coli; Escherichia coli; Genotoxic stress; Helicase; MMR; NER; PUR; PcrA UvrD Rep; Plants; Rice; base excision repair; direct repair; mismatch repair; nucleotide excision repair