title: TRAIL is involved in the tumoricidal activity of mouse natural killer cells stimulated by Newcastle disease virus in vitro.
abstract: Newcastle disease virus (NDV) is a potential antitumor agent, and its antitumor effect has been evaluated in preclinical tests. However, the mechanisms of NDV-based antitumor therapy are still not completely clear. In the present study we found that NDV-stimulation enhanced the killing ability of mouse spleen natural killer (NK) cells towards mouse hepatoma cell lines, and tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) plays an important role in this tumoricidal activity. NDV stimulation induced up-regulation of TRAIL both at the mRNA and protein levels in NK cells. Blocking TRAIL by antibody (Ab) almost completely eliminated the killing effect of NK cells on hepatoma cell lines. Furthermore, neutralizing interferon (IFN)-γ by Ab could inhibit TRAIL expression and tumoricidal activity of NDV-stimulated NK cells. These results indicated a substantial role of TRAIL as an effector molecule in NDV-induced NK cells mediated tumoricidal activity. The NDV stimulation triggered TRAIL expression in mouse spleen NK cells could be mediated by IFN-γ induction.
authors: Department of Microbiology, Guangxi Medical University, 22 Shuangyong Road, Nanning, 530021, Guangxi, China.
authors: De-Zhi
authors: DZ
authors: Song
authors: Ying
authors: Y
authors: Liang
authors: Qing
authors: Q
authors: Xiao
authors: Jun
authors: J
authors: Yin
authors: Jin-Ling
authors: JL
authors: Gong
authors: Zhen-Ping
authors: ZP
authors: Lai
authors: Zeng-Feng
authors: ZF
authors: Zhang
authors: Ling-Xi
authors: LX
authors: Gao
authors: Xiao-Hui
authors: XH
authors: Fan
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D015153:Blotting, Western; D006528:Carcinoma, Hepatocellular / Q000276:immunology / Q000517:prevention & control* / Q000821:virology; D003602:Cytotoxicity, Immunologic / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D066298:In Vitro Techniques; D007371:Interferon-gamma / Q000378:metabolism; D007694:Killer Cells, Natural / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism; D008113:Liver Neoplasms / Q000276:immunology / Q000517:prevention & control* / Q000821:virology; D051379:Mice; D008807:Mice, Inbred BALB C; D009522:Newcastle disease virus / Q000235:genetics / Q000276:immunology*; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013154:Spleen / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D053221:TNF-Related Apoptosis-Inducing Ligand / Q000037:antagonists & inhibitors / Q000276:immunology / Q000378:metabolism*; D014407:Tumor Cells, Cultured; D014779:Virus Replication / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D012333:RNA, Messenger; D053221:TNF-Related Apoptosis-Inducing Ligand; D007371:Interferon-gamma
keywords: NDV; NK cell; TRAIL; liver cancer