title: [Sequencing - classical method].
abstract: In this article the basic methods of reading nucleotide sequences in DNA molecules are summarized. Sanger sequencing is described most thoroughly as it is the most frequent routine method currently being utilized. The article describes in detail the principle of sequence determination through the production of fragments with a known end base using chain termination synthesis of DNA and ways of separation and detection of the fragments. Some alternative methods of sequencing are mentioned in short. Basic approaches of analyzing sequence data are explained as well as different outcomes, obstacles and challenges.
authors: Bioptická laboratoř, s.r.o., Plzeň. sedivcova@medima.cz
authors: Monika
authors: M
authors: Sedivcová
authors: Petr
authors: P
authors: Martínek
authors: Jan
authors: J
authors: Stehlík
authors: Petr
authors: P
authors: Grossmann
authors: Jana
authors: J
authors: Kašpírková
authors: Tomáš
authors: T
authors: Vaneček
mesh_terms: D000818:Animals; D004247:DNA / Q000032:analysis*; D006801:Humans; D017422:Sequence Analysis, DNA / Q000379:methods*
chemical_list: D004247:DNA