title: Gut microbiota, microinflammation, metabolic profile, and zonulin concentration in obese and normal weight subjects.
abstract: The association between gut microbiota and circulating zonulin level, a marker of intestinal permeability, has not been studied yet. The aim of the study is the assessment of plasma zonulin, haptoglobin and proinflammatory cytokines (TNF- α and IL-6) levels in relation to composition of gut microbiota in obese and normal weight subjects. Circulating inflammation markers, such as TNF- α , sTNFR1, sTNFR2, IL-6, zonulin, and haptoglobin levels were measured and semiquantitative analysis of gut microbiota composition was carried out in 50 obese and 30 normal weight subjects without concomitant diseases. Higher circulating zonulin, TNF- α , sTNFR1, sTNFR2, and IL-6 levels were found in the obese subjects. Plasma zonulin level correlated positively with age (r = 0.43, P < 0.001), body mass (r = 0.30, P < 0.01), BMI (r = 0.33, P < 0.01), fat mass and fat percentage (r = 0.31, P < 0.01 and r = 0.23, P < 0.05, resp.). Positive correlations between bacterial colony count and sTNFR1 (r = 0.33, P < 0.01) and plasma zonulin (r = 0.26, P < 0.05) but not haptoglobin levels were found. Additionally, plasma zonulin level was proportional to daily energy intake (r = 0.27, P < 0.05) and serum glucose concentration (r = 0.18, P < 0.05) and inversely proportional to diet protein percentage (r = -0.23, P < 0.05). Gut microbiota-related systemic microinflammation in the obese is reflected by circulating zonulin level, a potential marker of interstitial permeability.
authors: Pathophysiology Unit, Department of Pathophysiology, Medical University of Silesia, University of Silesia, 18 Medyków Street, 40-752 Katowice, Poland.
authors: Agnieszka
authors: A
authors: Zak-Gołąb
authors: Piotr
authors: P
authors: Kocełak
authors: Małgorzata
authors: M
authors: Aptekorz
authors: Maria
authors: M
authors: Zientara
authors: Lukasz
authors: L
authors: Juszczyk
authors: Gayane
authors: G
authors: Martirosian
authors: Jerzy
authors: J
authors: Chudek
authors: Magdalena
authors: M
authors: Olszanecka-Glinianowicz
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