title: Shoot organogenesis and plant regeneration from leaf explants of Lysionotus serratus D. Don.
abstract: The gesneriaceous perennial plant, Lysionotus serratus, has been used in traditional Chinese medicine. It also has a great development potential as an ornamental plant with its attractive foliage and beautiful flowers. An efficient propagation and regeneration system via direct shoot organogenesis from leaf explant was established in this study. High active cytokinin (6-benzyladenine (BA) or thidiazuron (TDZ)) was effective for direct organogenesis of initial induction. Murashige and Skoog (MS) growth media containing 0.5 mg L(-1) BA alone or with combination of 0.1 mg L(-1) α-Naphthaleneacetic acid (NAA) were the most effective for shoot proliferation. High BA concentration (1.0 mg L(-1)) in the media caused high percentage of vitrified shoots though they introduced high shoot proliferation rate. Histological observation indicated that adventitious shoot regeneration on the medium containing 0.5 mg L(-1) BA alone occurred directly from leaf epidermal cells without callus formation. Regenerated shoots rooted well on medium containing half-strength MS medium with 0.5 mg L(-1) indole-3-butyric acid (IBA) and indole-3-acetic acid (IAA), and the plantlets successfully acclimatized and grew vigorously in the greenhouse with a 94.2% and 92.1% survival rate.
authors: School of Ecology, Shanghai Institute of Technology, Shanghai 201418, China.
authors: Qiansheng
authors: Q
authors: Li
authors: Min
authors: M
authors: Deng
authors: Jie
authors: J
authors: Zhang
authors: Wei
authors: W
authors: Zhao
authors: Yigang
authors: Y
authors: Song
authors: Quanjian
authors: Q
authors: Li
authors: Qingjun
authors: Q
authors: Huang
mesh_terms: D003470:Culture Media; D019684:Magnoliopsida / Q000254:growth & development* / Q000502:physiology; D018515:Plant Leaves / Q000502:physiology*; D018520:Plant Shoots / Q000254:growth & development*; D012038:Regeneration
chemical_list: D003470:Culture Media
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