title: Expression of ECRG4 is associated with lower proliferative potential of esophageal cancer cells.
abstract: We have shown that ECRG4 suppressed Fas-induced apoptosis in Jurkat cells. ECRG4 mRNA expression was ubiquitously detected in normal adult human tissues, suggesting that ECRG4 plays a major role in human tissues. ECRG4 mRNA expression was down-regulated in tumor cells. Expression of ECRG4 suppressed cell growth. We established an anti-ECRG4 monoclonal antibody. Our immunohistochemical analysis demonstrated that ECRG4-positive cells tended to be distributed in the region that was negative for Ki-67 in esophageal squamous cell carcinoma tissues. There was a significant inverse correlation between ECRG4 expression and Ki-67 labeling index in esophageal squamous cell carcinoma. This study provides the first functional evidence for an association of endogenous expression of ECRG4 with cell proliferation. ECRG4 is a candidate tumor suppressor gene that might be involved in the proliferation of esophageal squamous cell carcinoma.
authors: Department of Pathology, Sapporo Medical University School of Medicine, Sapporo, Japan.
authors: Junichi
authors: J
authors: Matsuzaki
authors: Toshihiko
authors: T
authors: Torigoe
authors: Yoshihiko
authors: Y
authors: Hirohashi
authors: Yasuaki
authors: Y
authors: Tamura
authors: Hiroko
authors: H
authors: Asanuma
authors: Emiri
authors: E
authors: Nakazawa
authors: Eri
authors: E
authors: Saka
authors: Kazuyo
authors: K
authors: Yasuda
authors: Shuji
authors: S
authors: Takahashi
authors: Noriyuki
authors: N
authors: Sato
mesh_terms: D000368:Aged; D000911:Antibodies, Monoclonal / Q000276:immunology*; D002294:Carcinoma, Squamous Cell / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D004938:Esophageal Neoplasms / Q000378:metabolism* / Q000473:pathology; D000077277:Esophageal Squamous Cell Carcinoma; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D019394:Ki-67 Antigen / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D011379:Prognosis; D012333:RNA, Messenger / Q000378:metabolism; D025521:Tumor Suppressor Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism*
chemical_list: D000911:Antibodies, Monoclonal; C476999:ECRG4 protein, human; D019394:Ki-67 Antigen; D009363:Neoplasm Proteins; D012333:RNA, Messenger; D025521:Tumor Suppressor Proteins
keywords: Ki-67 labeling index; esophageal cancer-related gene 4; esophageal squamous cell carcinoma; tumor suppressor