title: [Role of uteroglobin-binding protein in antiflammin-1 promoting IL-10 expression and secretion in RAW264.7 cells induced by endotoxin].
abstract: The present study investigated the effect of antiflammin-1 (AF-1) on LPS-induced IL-10 secretion from RAW264.7 cells through uteroglobin-binding protein (UGBP). Cultured RAW264.7 cells, a murine monocyte-macrophage cell line, were divided as following: control group, LPS group (1 µg/mL LPS), AF-1 group (100 μmol/L AF-1), LPS+AF-1 group (2 h of 100 μmol/L AF-1 pretreatment before LPS addition), and LPS+AF-1+anti-UGBP group (30 min of anti-UGBP antibody pretreatment before successive treatments with AF-1 and LPS). IL-10 concentration in the supernatants was detected by ELISA assay, and the level of IL-10 mRNA expression in macrophage was detected by using RT-PCR method. The results showed that AF-1 significantly increased LPS-induced IL-10 secretion in RAW264.7 cells in a dose dependent way, and up-regulated its mRNA level. Anti-UGBP antibody pretreatment attenuated the augmented effect of AF-1 on LPS-induced IL-10 secretion and gene expression. These results suggest that AF-1 promotes LPS-induced IL-10 secretion from macrophages, and this effect is mediated by UGBP.
authors: Department of Physiology, Central South University, Changsha 410078, China; Department of Physiology, Xiangnan College, Chenzhou 423000, China; Department of Physiology, Changzhi Medical College, Changzhi 046000, China. E-mail: luozq1962@163.com; ivyandl.li@hotmail.com.
authors: Tian-Jie
authors: TJ
authors: Zhang
authors: Jian-Zhong
authors: JZ
authors: Han
authors: Hui-Jun
authors: HJ
authors: Liu
authors: Xiao-Hong
authors: XH
authors: Liao
authors: Chen
authors: C
authors: Li
authors: Zi-Qiang
authors: ZQ
authors: Luo
mesh_terms: D000818:Animals; D002460:Cell Line; D015870:Gene Expression; D016753:Interleukin-10 / Q000378:metabolism*; D008070:Lipopolysaccharides; D008264:Macrophages / Q000378:metabolism*; D051379:Mice; D010446:Peptide Fragments / Q000378:metabolism*; D012333:RNA, Messenger; D014598:Uteroglobin / Q000378:metabolism*
chemical_list: D008070:Lipopolysaccharides; D010446:Peptide Fragments; D012333:RNA, Messenger; C064645:antiflammin P1; D016753:Interleukin-10; D014598:Uteroglobin