title: Isolation and propagation of colon cancer stem cells.
abstract: The design of tissue culture conditions that faithfully reproduce the characteristics of cells in their native environment remains one of the main challenges of cancer stem cell (CSC) biology. Here we describe a detailed methodology for the isolation and expansion of both human colon CSCs and mouse intestinal adenoma together with a brief differentiation and coculture method that proved to be valuable to study the concept of CSCs plasticity.
authors: Laboratory of Experimental Oncology and Radiobiology Center for Experimental Molecular Medicine, Academic Medical Center, Amsterdam, The Netherlands.
authors: Pramudita R
authors: PR
authors: Prasetyanti
authors: Cheryl
authors: C
authors: Zimberlin
authors: Felipe
authors: F
authors: De Sousa E Melo
authors: Jan Paul
authors: JP
authors: Medema
mesh_terms: D000236:Adenoma / Q000473:pathology; D000818:Animals; D054337:Cell Dedifferentiation; D002454:Cell Differentiation; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002469:Cell Separation; D002478:Cells, Cultured; D018920:Coculture Techniques; D003110:Colonic Neoplasms / Q000473:pathology; D017077:Culture Media, Conditioned; D061252:Feeder Cells; D006801:Humans; D051379:Mice; D014411:Neoplastic Stem Cells / Q000502:physiology*
chemical_list: D017077:Culture Media, Conditioned