title: Impact of site-directed mutant luciferase on quantitative green and orange/red emission intensities in firefly bioluminescence.
abstract: Firefly bioluminescence has attracted great interest because of its high quantum yield and intriguing modifiable colours. Modifications to the structure of the enzyme luciferase can change the emission colour of firefly bioluminescence, and the mechanism of the colour change has been intensively studied by biochemists, structural biologists, optical physicists, and quantum-chemistry theorists. Here, we report on the quantitative spectra of firefly bioluminescence catalysed by wild-type and four site-directed mutant luciferases. While the mutation caused different emission spectra, the spectra differed only in the intensity of the green component (λmax ~ 560 nm). In contrast, the orange (λmax ~ 610 nm) and red (λmax ~ 650 nm) components present in all the spectra were almost unaffected by the modifications to the luciferases and changes in pH. Our results reveal that the intensity of the green component is the unique factor that is influenced by the luciferase structure and other reaction conditions.
authors: Institute for Solid State Physics, University of Tokyo, Kashiwanoha 5-1-5, Kashiwa, Chiba 2778584, Japan. wang@issp.u-tokyo.ac.jp
authors: Yu
authors: Y
authors: Wang
authors: Hidefumi
authors: H
authors: Akiyama
authors: Kanako
authors: K
authors: Terakado
authors: Toru
authors: T
authors: Nakatsu
mesh_terms: D000818:Animals; D003116:Color; D048888:Fireflies / Q000201:enzymology* / Q000235:genetics*; D049409:Luciferases, Firefly / Q000032:analysis / Q000737:chemistry* / Q000235:genetics*; D008163:Luminescent Measurements; D016297:Mutagenesis, Site-Directed; D013329:Structure-Activity Relationship
chemical_list: D049409:Luciferases, Firefly
references: Methods Enzymol. 2000;305:3-47
references: 10812588
references: J Phys Chem B. 2011 Jan 20;115(2):329-32
references: 21166405
references: Biochemistry. 1993 Dec 21;32(50):13795-9
references: 8268154
references: Cell Mol Life Sci. 2011 Apr;68(7):1167-82
references: 21188462
references: Nature. 2006 Mar 16;440(7082):372-6
references: 16541080
references: Photochem Photobiol. 2008 Jan-Feb;84(1):138-44
references: 18173713
references: Photochem Photobiol. 2010 Sep-Oct;86(5):1046-9
references: 20663080
references: Photochem Photobiol. 2011 Jul-Aug;87(4):846-52
references: 21477077
references: J Am Chem Soc. 2007 Jul 18;129(28):8756-65
references: 17585760
references: Arch Biochem Biophys. 1960 May;88:136-41
references: 14444706
references: Cell Mol Life Sci. 2002 Nov;59(11):1833-50
references: 12530517
references: Proc Natl Acad Sci U S A. 1964 Jul;52(1):75-81
references: 16591195
references: Biochemistry. 2004 Jun 15;43(23):7255-62
references: 15182171
references: Biochim Biophys Acta. 2002 Jun 3;1597(2):271-9
references: 12044905
references: Photochem Photobiol. 2007 Sep-Oct;83(5):1205-10
references: 17880516
references: J Am Chem Soc. 1969 Apr 9;91(8):2178-80
references: 5784183
references: Biochemistry (Mosc). 2005 Nov;70(11):1262-7
references: 16336187