title: Phenotypic detection of metallo-β-lactamases in Pseudomonas aeruginosa and Acinetobacter baumannii isolated from hospitalized patients in São Luis, State of Maranhão, Brazil.
abstract: BACKGROUND
  Acquired metallo-β-lactamases (MβL) are emerging determinants of resistance in Pseudomonas aeruginosa and Acinetobacter baumannii. The objectives of this study were to phenotypically detect MβL in imipenem-resistant P. aeruginosa and A. baumannii, to investigate the association between MβL-positive strains and hospitals, and to compare the resistance profiles of MβL-producing and non-MβL-producing strains.
  
  
  METHODS
  The approximation disk and combined disk assay methods were used in this study.
  
  
  RESULTS
  A total of 18 (38.3%) P. aeruginosa isolates and 1 (5.6%) A. baumannii isolate tested positive for the presence of MβL.
  
  
  CONCLUSIONS
  These results demonstrate the need for strict surveillance and for the adoption of preventive measures to reduce the spread of infection and potential outbreaks of disease caused by MβL-producing microorganisms.
authors: Programa de Pós-Graduação em Ciências da Saúde, Universidade Federal do Maranhão, São Luis, MA.
authors: Roberto Morais Luz de
authors: RM
authors: Carvalho
authors: Sirlei Garcia
authors: SG
authors: Marques
authors: Luís Henrique Bastos
authors: LH
authors: Gonçalves
authors: Afonso Gomes
authors: AG
authors: Abreu
authors: Silvio Gomes
authors: SG
authors: Monteiro
authors: Azizedite Guedes
authors: AG
authors: Gonçalves
mesh_terms: D040981:Acinetobacter baumannii / Q000187:drug effects / Q000201:enzymology* / Q000302:isolation & purification; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D052978:Disk Diffusion Antimicrobial Tests; D006801:Humans; D010641:Phenotype; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000201:enzymology* / Q000302:isolation & purification; D018440:beta-Lactam Resistance / Q000235:genetics*; D001618:beta-Lactamases / Q000096:biosynthesis / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D000900:Anti-Bacterial Agents; D001618:beta-Lactamases