title: Dietary grape seed extract ameliorates symptoms of inflammatory bowel disease in IL10-deficient mice.
abstract: Grape seed extract (GSE) is a by-product of the wine industry, with abundant polyphenolic compounds known for their anti-inflammatory and anti-oxidative effects. Using IL10-deficient mice (IL10KO), here we showed that GSE (1% of dry feed weight) ameliorated inflammatory bowel disease indices, increased colonic goblet cell numbers and decreased myeloperoxidase levels in the large intestine. Concomitantly, GSE supplementation attenuated inflammation, decreased the expression of pore forming tight junction protein claudin2, and increased levels of Lactobacilli and Bacteroides in the gut microbiota of IL10KO mice. In summary, our study shows that GSE has protective roles on inflammatory bowel disease through altering gut inflammation, tight junction protein expression, and gut microbiota composition.
authors: Department of Animal Science, University of Wyoming, Laramie, WY, USA.
authors: Hui
authors: H
authors: Wang
authors: Yansong
authors: Y
authors: Xue
authors: Hanying
authors: H
authors: Zhang
authors: Yan
authors: Y
authors: Huang
authors: Guan
authors: G
authors: Yang
authors: Min
authors: M
authors: Du
authors: Mei-Jun
authors: MJ
authors: Zhu
mesh_terms: D000818:Animals; D001439:Bacteroides / Q000187:drug effects; D003106:Colon / Q000166:cytology / Q000187:drug effects; D041981:Gastrointestinal Tract / Q000187:drug effects / Q000382:microbiology; D020397:Goblet Cells / Q000187:drug effects; D056604:Grape Seed Extract / Q000494:pharmacology*; D015212:Inflammatory Bowel Diseases / Q000178:diet therapy* / Q000382:microbiology / Q000473:pathology; D007371:Interferon-gamma / Q000235:genetics; D016753:Interleukin-10 / Q000235:genetics* / Q000378:metabolism; D015850:Interleukin-6 / Q000235:genetics; D007778:Lactobacillus / Q000187:drug effects; D051379:Mice; D018345:Mice, Knockout; D064307:Microbiota; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics
chemical_list: D056604:Grape Seed Extract; C556062:IL10 protein, mouse; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D007371:Interferon-gamma
keywords: Epithelium; Grape seed extract; IL-10; Inflammatory bowel disease; Intestine; Microbiota
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