title: Repair of rabbit segmental femoral defects by using a combination of tetrapod-shaped calcium phosphate granules and basic fibroblast growth factor-binding ion complex gel.
abstract: The effect of tetrapod-shaped alpha tricalcium phosphate granules (TB) as a scaffold combined with basic fibroblast growth factor (bFGF)-binding ion complex gel (f-IC gel) on neovascularization and bone regeneration was evaluated in segmental femoral defects of rabbits. The defects were stabilized using a plate with a polypropylene mesh cage (PMC) containing one of the following: PMC alone (PMC group), TB (TB group), TB and bFGF (TB/f group), TB and IC gel (TB/IC group), or TB and f-IC gel (TB/f-IC group). Four rabbits from each group were euthanized at 2 and 4 weeks after surgery. Histomorphometry showed that the number of vessels and the volume of new bone in the TB/f-IC group were significantly higher than those in the other groups at all time points. There were no differences in the extent of neovascularization and new bone formation between the TB and TB/f groups. These findings suggest that the combination of TB and f-IC gel facilitated both neovascularization and new bone formation in segmental femoral defects of rabbits. This combination may be of considerable use for treating segmental long bone defects.
authors: Laboratory of Veterinary Surgery, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan; Department of Bioengineering, Graduate School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8655, Japan.
authors: Muneki
authors: M
authors: Honnami
authors: Sungjin
authors: S
authors: Choi
authors: I-Li
authors: IL
authors: Liu
authors: Wataru
authors: W
authors: Kamimura
authors: Tetsushi
authors: T
authors: Taguchi
authors: Hironori
authors: H
authors: Hojo
authors: Nobuyuki
authors: N
authors: Shimohata
authors: Shinsuke
authors: S
authors: Ohba
authors: Hiroyuki
authors: H
authors: Koyama
authors: Ryohei
authors: R
authors: Nishimura
authors: Ung-il
authors: UI
authors: Chung
authors: Nobuo
authors: N
authors: Sasaki
authors: Manabu
authors: M
authors: Mochizuki
mesh_terms: D000818:Animals; D002130:Calcium Phosphates / Q000737:chemistry* / Q000494:pharmacology*; D005269:Femur / Q000000981:diagnostic imaging / Q000187:drug effects / Q000473:pathology*; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology*; D005782:Gels / Q000494:pharmacology*; D006801:Humans; D020007:Implants, Experimental; D007477:Ions; D008297:Male; D010012:Osteogenesis / Q000187:drug effects; D011817:Rabbits; D014945:Wound Healing / Q000187:drug effects*; D055114:X-Ray Microtomography
chemical_list: D002130:Calcium Phosphates; D005782:Gels; D007477:Ions; C485828:alpha-tricalcium phosphate; D016222:Fibroblast Growth Factor 2
keywords: Animal model; Bone regeneration; Calcium phosphate; Collagen; Fibroblast growth factor; Scaffold