title: Characterization of growth and Oryctes rhinoceros nudivirus production in attached cultures of the DSIR-HA-1179 coleopteran insect cell line.
abstract: The DSIR-HA-1179 coleopteran cell line is a susceptible and permissive host to the Oryctes rhinoceros nudivirus (OrNV), which has been used as a biocontrol agent against the coconut rhinoceros beetle (Oryctes rhinoceros); a pest of palms in the Asia-Pacific region. However, little is known about growth and metabolism of this cell line, knowledge of which is necessary to develop an in vitro large-scale OrNV production process. The strong anchorage-dependent characteristics of the cell line, its particular fragility and its tendency to form dense clumps when manipulated, are the most likely reasons that have precluded further development of the cell line. In order to characterize DSIR-HA-1179 cells, there was first a need for a reliable technique to count the cells. A homogenous cell suspension suitable for enumeration could be produced by treatment with TrypLE Express™ with optimum mean time for cell release calculated as 30 min. The cell line was adapted to grow in four serum-supplemented culture media namely TC-100, IPL-41, Sf-900 II and Sf-900 III and cell growth, glucose consumption, lactate and ammonia production were assessed from static-batch cultures. The maximum viable cell density was reached in Sf-900 II (17.9 × 10(5) cells/ml), with the maximum specific growth rate observed in this culture medium as well (0.0074 h(-1)). Higher production of OrNV was observed in IPL-41 and TC-100 (4.1 × 10(7) TCID50/ml) than in cultures infected in Sf-900 III (2.0 × 10(7) TCID50/ml) and Sf-900 II (1.4 × 10(7) TCID50/ml). At the end of the growth period, glucose was completely consumed in cultures grown in TC-100, while remained in excess in the other three culture media. The cell line produced lactate and ammonia to very low levels in the TC-100 culture medium which is a promising aspect for its cultivation at large-scale.
authors: Department of Chemical and Process Engineering, University of Canterbury, Christchurch, 8140, New Zealand.
authors: Charlotte
authors: C
authors: Pushparajan
authors: Juan Daniel
authors: JD
authors: Claus
authors: Sean David Goldie
authors: SD
authors: Marshall
authors: Gabriel
authors: G
authors: Visnovsky
references: Trends Biotechnol. 1991 Dec;9(12):427-37
references: 1367747
references: Appl Microbiol Biotechnol. 1995 Nov;43(6):1006-13
references: 8590651
references: Virology. 1981 Jul 30;112(2):625-33
references: 18635075
references: Crit Rev Biotechnol. 2001;21(2):67-110
references: 11451047
references: Mol Ecol. 1999 Sep;8(9):1552-3
references: 10617346
references: J Gen Virol. 1974 Oct;25(1):105-16
references: 4430894
references: Biotechnol Bioeng. 1996 Dec 20;52(6):696-706
references: 18629948
references: Cytotechnology. 1996 Jan;21(1):73-80
references: 22358608
references: J Virol Methods. 2011 Dec;178(1-2):106-16
references: 21906626
references: Biotechnol Adv. 2012 Sep-Oct;30(5):1140-57
references: 21983546
references: J Biotechnol. 2000 Feb 28;78(1):23-37
references: 10702908
references: Biotechnol Bioeng. 1997 Sep 20;55(6):909-20
references: 18636600
references: Cytotechnology. 2008 May;57(1):23-35
references: 19003169
references: In Vitro Cell Dev Biol Anim. 2009 Jan-Feb;45(1-2):19-22
references: 18946707
references: Cytotechnology. 2006 Oct;52(2):113-24
references: 19002870
references: Virus Genes. 2011 Jun;42(3):444-56
references: 21380757
references: Cytotechnology. 2003 Sep;42(3):109-20
references: 19002933
references: Mol Biotechnol. 2011 Jan;47(1):9-17
references: 20589456
references: J Virol Methods. 1989 Apr-May;24(1-2):203-13
references: 2760162
references: Ann N Y Acad Sci. 1992 Oct 13;665:230-7
references: 1416605
references: Exp Cell Res. 1972 Sep;74(1):51-60
references: 4627417
references: Cytotechnology. 2001 Nov;37(3):189-98
references: 19002922
references: In Vitro Cell Dev Biol Anim. 2012 Aug;48(7):426-33
references: 22752637
references: Biotechnol Prog. 1998 Jul-Aug;14(4):543-7
references: 9694673
references: J Invertebr Pathol. 2005 May;89(1):78-84
references: 16039308
references: Cytotechnology. 1996 Jan;20(1-3):43-56
references: 22358473
references: Virology. 1990 Jan;174(1):294-8
references: 2403706
references: J Invertebr Pathol. 2005 May;89(1):91-4
references: 16039310
references: Biotechnol Bioeng. 1996 Aug 20;51(4):494-9
references: 18629802
references: Enzyme Microb Technol. 2000 Jul 1;27(1-2):43-52
references: 10862901