title: The GMOseek matrix: a decision support tool for optimizing the detection of genetically modified plants.
abstract: BACKGROUND
  Since their first commercialization, the diversity of taxa and the genetic composition of transgene sequences in genetically modified plants (GMOs) are constantly increasing. To date, the detection of GMOs and derived products is commonly performed by PCR-based methods targeting specific DNA sequences introduced into the host genome. Information available regarding the GMOs' molecular characterization is dispersed and not appropriately organized. For this reason, GMO testing is very challenging and requires more complex screening strategies and decision making schemes, demanding in return the use of efficient bioinformatics tools relying on reliable information.
  
  
  METHODS
  The GMOseek matrix was built as a comprehensive, online open-access tabulated database which provides a reliable, comprehensive and user-friendly overview of 328 GMO events and 247 different genetic elements (status: 18/07/2013). The GMOseek matrix is aiming to facilitate GMO detection from plant origin at different phases of the analysis. It assists in selecting the targets for a screening analysis, interpreting the screening results, checking the occurrence of a screening element in a group of selected GMOs, identifying gaps in the available pool of GMO detection methods, and designing a decision tree. The GMOseek matrix is an independent database with effective functionalities in a format facilitating transferability to other platforms. Data were collected from all available sources and experimentally tested where detection methods and certified reference materials (CRMs) were available.
  
  
  CONCLUSIONS
  The GMOseek matrix is currently a unique and very valuable tool with reliable information on GMOs from plant origin and their present genetic elements that enables further development of appropriate strategies for GMO detection. It is flexible enough to be further updated with new information and integrated in different applications and platforms.
authors: Walloon Agricultural Research Centre (CRA-W), Gembloux, Belgium. debode@cra.wallonie.be.
authors: Annette
authors: A
authors: Block
authors: Frédéric
authors: F
authors: Debode
authors: Lutz
authors: L
authors: Grohmann
authors: Julie
authors: J
authors: Hulin
authors: Isabel
authors: I
authors: Taverniers
authors: Linda
authors: L
authors: Kluga
authors: Elodie
authors: E
authors: Barbau-Piednoir
authors: Sylvia
authors: S
authors: Broeders
authors: Ingrid
authors: I
authors: Huber
authors: Marc
authors: M
authors: Van den Bulcke
authors: Petra
authors: P
authors: Heinze
authors: Gilbert
authors: G
authors: Berben
authors: Ulrich
authors: U
authors: Busch
authors: Nancy
authors: N
authors: Roosens
authors: Eric
authors: E
authors: Janssen
authors: Jana
authors: J
authors: Žel
authors: Kristina
authors: K
authors: Gruden
authors: Dany
authors: D
authors: Morisset
mesh_terms: D001483:Base Sequence; D003661:Decision Support Techniques; D017343:Genes, Plant; D030821:Plants, Genetically Modified / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods
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