title: Vegetative Growth and Phylogenetic Relationship of Commercially Cultivated Strains of Pleurotus eryngii based on ITS sequence and RAPD.
abstract: Pleurotus eryngii, known as king oyster mushroom has been widely used for nutritional and medicinal purposes. This study was initiated to screen the suitable conditions for mycelial growth and to determine the phylogenetic relationship of the selected strains. Optimal mycelial growth was observed at 30℃ and minimum mycelial growth observed at 10℃. This mushroom tolerates a broad pH range for mycelial growth, with most favorable growth observed at pH 6. Results also indicated that glucose peptone, yeast malt extract and mushroom complete media were favorable growth media, while Hennerberg and Hoppkins media were unfavorable. Dextrin was the best and xylose the least effective carbon sources. Results revealed that inorganic nitrogen sources were less effective than organic sources for the mycelial growth of P. eryngii. Investigation of genetic diversity is necessary to identify the strains. The ITS region of rDNA were amplified using PCR. The size of the ITS1 and ITS2 regions of rDNA from the different strains varied from 214 to 222 bp and 145 to 236 bp, respectively. The sequence of ITS2 was more variable than that of ITS1, and the 5.8S sequences were identical. A phylogenetic tree based on the ITS region sequences indicated that selected strains could be classified into six clusters. Fourteen IUM and ATCC-90212 strains were also analyzed by RAPD with 20 arbitrary primers. Fourteen of these primers were efficiently amplified the genomic DNA. The number of amplified bands varied with the primers and strains, with polymorphic fragments in the range from 0.2 to 2.3 kb.
authors: Department of Biology, University of Incheon, Incheon 406-840, Korea.
authors: Nuhu
authors: N
authors: Alam
authors: Mi Ja
authors: MJ
authors: Shim
authors: Min Woong
authors: MW
authors: Lee
authors: Pyung Gyun
authors: PG
authors: Shin
authors: Young Bok
authors: YB
authors: Yoo
authors: Tae Soo
authors: TS
authors: Lee
keywords: ITS; Phylogenetic relation; Pleurotus eryngii; RAPD; Vegetative growth
references: Mycobiology. 2008 Jun;36(2):81-7
references: 23990738
references: Microbiology (Reading). 2001 Jul;147(Pt 7):1755-1763
references: 11429453
references: Microbiology (Reading). 2001 Nov;147(Pt 11):3183-94
references: 11700370
references: Mol Biol Evol. 1987 Jul;4(4):406-25
references: 3447015
references: Mycobiology. 2005 Mar;33(1):15-8
references: 24049468
references: Nucleic Acids Res. 1990 Nov 25;18(22):6531-5
references: 1979162
references: Evolution. 1985 Jul;39(4):783-791
references: 28561359
references: Nucleic Acids Res. 1994 Nov 11;22(22):4673-80
references: 7984417
references: Microbiology (Reading). 2005 May;151(Pt 5):1557-1567
references: 15870465
references: Mycobiology. 2009 Sep;37(3):183-8
references: 23983530
references: Proc Natl Acad Sci U S A. 1979 Oct;76(10):5269-73
references: 291943
references: Mycol Res. 2007 Jun;111(Pt 6):710-5
references: 17604148
references: Phytochemistry. 2002 Jul;60(6):551-65
references: 12126701