title: Ellipsoidal Polyaspartamide Polymersomes with Enhanced Cell-Targeting Ability.
abstract: Nano-sized polymersomes functionalized with peptides or proteins are being increasingly studied for targeted delivery of diagnostic and therapeutic molecules. Earlier computational studies have suggested that ellipsoidal nanoparticles, compared to spherical ones, display enhanced binding efficiency with target cells, but this has not yet been experimentally validated. We hypothesize that hydrophilic polymer chains coupled to vesicle-forming polymers would result in ellipsoidal polymersomes. In addition, ellipsoidal polymersomes modified with cell adhesion peptides bind with target cells more actively than spherical ones. We examine this hypothesis by substituting polyaspartamide with octadecyl chains and varying numbers of poly(ethylene glycol) (PEG) chains. Increasing the degree of substitution of PEG from 0.5 to 1.0 mol% drives the polymer to self-assemble into an ellipsoidal polymersome with an aspect ratio of 2.1. Further modification of these ellipsoidal polymersomes with peptides containing an Arg-Gly-Asp sequence (RGD peptides) lead to a significant increase in the rate of association and decrease in the rate of dissociation with a substrate coated with αvβ3integrins. In addition, in a circulation-mimicking flow, the ellipsoidal polymersomes linked with RGD peptides adhere to target tissues more favorably than their spherical equivalents do. Overall, the results of this study will greatly serve to improve the efficiency of targeted delivery of a wide array of polymersomes loaded with various biomedical modalities.
authors: Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign, 600 South Matthews Avenue, Urbana IL, 61801, USA.
authors: Mei-Hsiu
authors: MH
authors: Lai
authors: Jae Hyun
authors: JH
authors: Jeong
authors: Ross J
authors: RJ
authors: Devolder
authors: Christopher
authors: C
authors: Brockman
authors: Charles
authors: C
authors: Schroeder
authors: Hyunjoon
authors: H
authors: Kong
keywords: Biomedical Applications; Bionanotechnology; Polymeric Materials; Self-Assembly; Surface Plasmon Resonance
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