title: Chemistry and Antiviral Activity of Arrabidaea pulchra (Bignoniaceae).
abstract: The aim of the present work was to carry out a bioguided isolation of antiviral chemical constituents from an ethanol extract of leaves from Arrabidaea pulchra (Cham.) Sandwith (EEAPL) that had shown in vitro activity in a previous screening using DNA and RNA viruses. The activity of EEPAL was evaluated against the DNA viruses Human herpesvirus 1 (HSV-1) and Vaccinia virus Western Reserve (VACV-WR) as well as against the RNA viruses Murine encephalomyocarditis virus (EMCV), and Dengue virus 2 (DENV-2) by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) colorimetric assay. Cytotoxicity was determined in LLCMK2 and Vero cells and the Selectivity Indexes (SI) were calculated. The most potent effect was observed against DENV-2 (EC50 46.8 ± 1.6 µg mL(-1); SI 2.7). For HSV-1 and VACV-WR EC50 values > 200 µg mL(-1) were determined, while no inhibition of the cytopathic effect was observed with EMCV. Bioguided fractionation of EEAPL by partition between immiscible solvents followed by chromatography over a Sephadex LH20 column afforded two arylpropanoid glycosides, verbascoside (AP 1) and caffeoylcalleryanin (AP 2), along with a terpenoid, ursolic acid (AP 3). AP 1 and AP 3 exhibited similar anti-DENV-2 profiles, with SI values of 3.8 and 3.1, respectively, while AP 2 was the most effective anti-DENV-2 constituent, with a SI of 20.0. Our results show that A. pulchra leaves ethanol extract (EEAPL) affords compounds with antiviral activity, mainly against DENV-2.
authors: Faculdade de Farmácia, Departamento de Produtos Farmacêuticos, Universidade Federal de Minas Gerais, Av Antônio Carlos, 6627 CEP 31.270-901 Belo Horizonte, MG, Brazil.
authors: Geraldo Célio
authors: GC
authors: Brandão
authors: Erna G
authors: EG
authors: Kroon
authors: Danielle E R
authors: DE
authors: Souza
authors: José D
authors: JD
authors: Souza Filho
authors: Alaíde Braga
authors: AB
authors: Oliveira
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000737:chemistry*; D029402:Bignoniaceae / Q000737:chemistry*; D002522:Chlorocebus aethiops; D003716:Dengue Virus / Q000187:drug effects; D005960:Glucosides / Q000737:chemistry; D006027:Glycosides / Q000737:chemistry / Q000494:pharmacology; D006801:Humans; D010636:Phenols / Q000737:chemistry; D013729:Terpenes / Q000737:chemistry; D014315:Triterpenes / Q000737:chemistry; D014709:Vero Cells; D000097245:Ursolic Acid; D059808:Polyphenols
chemical_list: D000998:Antiviral Agents; D005960:Glucosides; D006027:Glycosides; D010636:Phenols; D013729:Terpenes; D014315:Triterpenes; D000097245:Ursolic Acid; C058956:acteoside; D059808:Polyphenols
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