title: Engineering and utilization of reporter cell lines for cell-based assays of transmembrane receptors.
abstract: Transmembrane receptors, a subset of integral membrane proteins, are the receivers that transduce an extracellular chemical message into an intracellular response. Accordingly, these proteins are of particular interest in the scientific community and are probably best studied as part of a cellular system. Herein, we detail the engineering of a fluorescent and bioluminescent reporter cell line for a transmembrane receptor and how to employ it in a directed evolution screen that identifies peptide regulators of receptor activity.
authors: Department of Chemistry and Biochemistry and the BioFrontiers Institute, University of Colorado at Boulder, Boulder, CO, USA.
authors: Matthew W
authors: MW
authors: Lluis
authors: Hang
authors: H
authors: Yin
mesh_terms: D000595:Amino Acid Sequence; D005456:Fluorescent Dyes / Q000737:chemistry*; D057809:HEK293 Cells; D006801:Humans; D008565:Membrane Proteins / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D015202:Protein Engineering / Q000379:methods*
chemical_list: D005456:Fluorescent Dyes; D008565:Membrane Proteins