title: Borrelia burgdorferi BbHtrA degrades host ECM proteins and stimulates release of inflammatory cytokines in vitro.
abstract: The Lyme disease spirochaete, Borrelia burgdorferi, causes damage to diverse host tissues and induces inflammation but the mechanisms of injury are poorly understood. We recently reported that a surface-exposed B. burgdorferi protease, which is expressed during human disease and is conserved within the major Lyme disease spirochaete species, degrades the extracellular matrix proteoglycan, aggrecan. Here we demonstrate that BbHtrA also degrades fibronectin and numerous proteoglycans found in skin, joints and neural tissues. BbHtrA degradation of fibronectin released known pro-inflammatory fibronectin fragments FnIII(13-14) and Fnf-29, which may amplify the inflammatory processes triggered by the presence of the bacteria. When this hypothesis was tested directly by exposing chondrocytes to BbHtrA in vitro, inflammatory cytokines (sICAM-1 and IL-6) and chemokines (CXCL1, CCL1, CCL2 and CCL5) that are hallmarks of Lyme disease were induced. These results provide the first evidence that, by utilizing BbHtrA, B. burgdorferi may actively participate in its dissemination and in the tissue damage and inflammation observed in Lyme disease.
authors: Division of Vector-Borne Diseases, Centers for Disease Control and Prevention, Fort Collins, CO, USA.
authors: Theresa M
authors: TM
authors: Russell
authors: Mark J
authors: MJ
authors: Delorey
authors: Barbara J B
authors: BJ
authors: Johnson
mesh_terms: D053674:Aggrecans / Q000378:metabolism; D025065:Borrelia burgdorferi / Q000378:metabolism*; D002478:Cells, Cultured; D019902:Chondrocytes / Q000276:immunology / Q000378:metabolism; D016207:Cytokines / Q000276:immunology*; D016326:Extracellular Matrix Proteins / Q000378:metabolism*; D005353:Fibronectins / Q000378:metabolism*; D006801:Humans; D007249:Inflammation / Q000276:immunology* / Q000378:metabolism; D007596:Joints / Q000378:metabolism; D008193:Lyme Disease / Q000276:immunology / Q000378:metabolism / Q000382:microbiology*; D011509:Proteoglycans / Q000378:metabolism*; D057057:Serine Proteases / Q000378:metabolism*; D012867:Skin / Q000378:metabolism
chemical_list: D053674:Aggrecans; D016207:Cytokines; D016326:Extracellular Matrix Proteins; D005353:Fibronectins; D011509:Proteoglycans; D057057:Serine Proteases