title: Novel alternative splice variants of chicken NPAS3 are expressed in the developing central nervous system.
abstract: We report isolation of novel splice variants of chicken Neuronal Per-Arnt-Sim domain protein 3 (cNPAS3) gene distinct from the previously predicted cNPAS3 at the 5' end. Newly identified cNPAS3 splice variants feature N-terminus coding sequences with high degrees of homology to human NPAS3 (hNAPS3). We also show that the alternative splicing pattern of NPAS3 is conserved between chicken and human. RNA in situ hybridization indicated that the expression of cNPAS3 in the developing central nervous system (CNS) is limited to the ventricular zone and only partially overlaps with that of chicken Reelin (cReelin), the only known regulatory target gene of NPAS3 in the adult brain. Overexpression of cNPAS3 by in ovo electroporation had little effect on the expression of Sox2, a marker for neural precursors, or of Isl1/2, a marker for early differentiating motor neurons. Taken together with the little effect of cNPAS3 overexpression on cReelin, it is noted that the function of NPAS3 in the developing CNS remains to be determined. Still, identification of proper cDNA sequences for cNPAS3 should represent a solid beginning of the understanding process.
authors: Department of Life Sciences and Ewha Research Center for Systems Biology, Ewha Womans University, Seoul 120-750, Korea.
authors: Jiheon
authors: J
authors: Shin
authors: Jaesang
authors: J
authors: Kim
mesh_terms: D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D051792:Basic Helix-Loop-Helix Proteins / Q000235:genetics* / Q000378:metabolism; D015816:Cell Adhesion Molecules, Neuronal / Q000235:genetics / Q000378:metabolism; D002490:Central Nervous System / Q000166:cytology / Q000254:growth & development / Q000378:metabolism*; D002645:Chickens / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D016326:Extracellular Matrix Proteins / Q000235:genetics / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D018257:Helix-Loop-Helix Motifs; D006801:Humans; D060850:LIM-Homeodomain Proteins / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D009046:Motor Neurons / Q000166:cytology / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism; D017434:Protein Structure, Tertiary; D000091042:Reelin Protein; D055748:SOXB1 Transcription Factors / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D012697:Serine Endopeptidases / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
chemical_list: D051792:Basic Helix-Loop-Helix Proteins; D015816:Cell Adhesion Molecules, Neuronal; D016326:Extracellular Matrix Proteins; D060850:LIM-Homeodomain Proteins; D009419:Nerve Tissue Proteins; D000091042:Reelin Protein; D055748:SOXB1 Transcription Factors; D012697:Serine Endopeptidases; D014157:Transcription Factors; C502963:NPAS3 protein, human; C000717127:RELN protein, human
keywords: AS; Alternative splice variants; CNS; HH stage; Hamburger–Hamilton stage; IgG; Insulin gene enhancer protein 1/2 (Islet1/2); Isl1/2; NPAS3; Neuronal Per–Arnt–Sim domain protein 3; ORF; PAS; PCR; Per–Arnt–Sim; RACE; RT-PCR; Reelin; S; SRY (sex determining region Y)-box 2; Sox2; UTR; antisense; bHLH; basic helix–loop–helix; cDNA; central nervous system; complementary DNA; immunoglobulin G; open reading frame; polymerase chain reaction; rapid amplification of cDNA ends; reverse transcription-polymerase chain reaction; sense; untranslated region