title: Gene-specific transcriptional mechanisms at the histone gene cluster revealed by single-cell imaging.
abstract: To bridge the gap between in vivo and in vitro molecular mechanisms, we dissected the transcriptional control of the endogenous histone gene cluster (His-C) by single-cell imaging. A combination of quantitative immunofluorescence, RNA FISH, and FRAP measurements revealed atypical promoter recognition complexes and differential transcription kinetics directing histone H1 versus core histone gene expression. While H1 is transcribed throughout S phase, core histones are only transcribed in a short pulse during early S phase. Surprisingly, no TFIIB or TFIID was detectable or functionally required at the initiation complexes of these promoters. Instead, a highly stable, preloaded TBP/TFIIA "pioneer" complex primes the rapid initiation of His-C transcription during early S phase. These results provide mechanistic insights for the role of gene-specific core promoter factors and implications for cell cycle-regulated gene expression.
authors: Howard Hughes Medical Institute, Department of Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA.
authors: Benjamin
authors: B
authors: Guglielmi
authors: Natalie
authors: N
authors: La Rochelle
authors: Robert
authors: R
authors: Tjian
mesh_terms: D000818:Animals; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D029721:Drosophila Proteins / Q000378:metabolism; D004331:Drosophila melanogaster / Q000235:genetics* / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D005786:Gene Expression Regulation; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006657:Histones / Q000235:genetics* / Q000378:metabolism; D007091:Image Processing, Computer-Assisted; D007700:Kinetics; D005810:Multigene Family; D011401:Promoter Regions, Genetic / Q000235:genetics; D016196:S Phase / Q000502:physiology*; D016385:TATA Box / Q000235:genetics; D035181:TATA-Box Binding Protein / Q000235:genetics / Q000378:metabolism*; D035341:Telomeric Repeat Binding Protein 2 / Q000378:metabolism; D035561:Transcription Factor TFIIA / Q000235:genetics / Q000378:metabolism; D035581:Transcription Factor TFIIB / Q000235:genetics / Q000378:metabolism; D035362:Transcription Factor TFIID / Q000235:genetics / Q000378:metabolism; D061785:Transcription Initiation, Genetic; D014158:Transcription, Genetic
chemical_list: D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D006657:Histones; D035181:TATA-Box Binding Protein; C509085:TRF2 protein, Drosophila; D035341:Telomeric Repeat Binding Protein 2; D035561:Transcription Factor TFIIA; D035581:Transcription Factor TFIIB; D035362:Transcription Factor TFIID; D049452:Green Fluorescent Proteins
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