title: Effect of pulverized natural bone mineral on regeneration of three-wall intrabony defects. A preclinical study.
abstract: OBJECTIVE
  The objective of this study is to evaluate the effects of a paste-like bone substitute material with easy handling properties and improved mechanical stability on periodontal regeneration of intrabony defects in dogs.
  
  
  METHODS
  Mandibular and maxillary first and third premolars were extracted, and three-wall intrabony defects were created on second and fourth premolars. After a healing period of 3 months, acute type defects were filled with a paste-like formulation of deproteinized bovine bone mineral (DBBM) (particle size, 0.125-0.25 mm) in a collagenous carrier matrix (T1), pulverized DBBM (particle size, 0.125-0.25 mm) without the carrier (T2), or Bio-Oss® granules (particle size, 0.25-1.00 mm) as control (C). All defects were covered with a Bio-Gide® membrane. The dogs were sacrificed after 12 weeks, and the specimens were analyzed histologically and histometrically.
  
  
  RESULTS
  Postoperative healing of all defects was uneventful, and no histological signs of inflammation were observed in the augmented and gingival regions. New cementum, new periodontal ligament, and new bone were observed in all three groups. The mean vertical bone gain was 3.26 mm (T1), 3.60 mm (T2), and 3.81 mm (C). That of new cementum was 2.25 mm (T1), 3.88 mm (T2), and 3.53 mm (C). The differences did not reach statistical significance. The DBBM particles were both incorporated in new bone and embedded in immature bone marrow.
  
  
  CONCLUSIONS
  The results of this preclinical study showed that the 0.125-0.25-mm DBBM particles in a powder or paste formulation resulted in periodontal regeneration comparable to the commercially available DBBM. Osteoconductivity, in particular, was not affected by DBBM size or paste formulation.
  
  
  CONCLUSIONS
  The improved handling properties of the paste-like bone substitute consisting of small DBBM particles embedded in a collagen-based carrier hold promise for clinical applications.
authors: Department of Periodontology, School of Dental Medicine, University of Bern, Freiburgstrasse 7, 3010, Bern, Switzerland.
authors: A
authors: A
authors: Ivanovic
authors: Department of Periodontology, School of Dental Medicine, University of Bern, Freiburgstrasse 7, 3010, Bern, Switzerland.|Department of Oral Surgery and Stomatology, School of Dental Medicine, University of Bern, Bern, Switzerland.|Robert K. Schenk Laboratory of Oral Histology, School of Dental University of Bern, Bern, Switzerland.
authors: D D
authors: DD
authors: Bosshardt
authors: Department of Oral Surgery, Heinrich Heine University, Düsseldorf, Germany.
authors: I
authors: I
authors: Mihatovic
authors: Department of Oral Surgery, Heinrich Heine University, Düsseldorf, Germany.
authors: F
authors: F
authors: Schwarz
authors: Department of Periodontology, School of Dental Medicine, University of Bern, Freiburgstrasse 7, 3010, Bern, Switzerland.|Department of Oral Surgery and Stomatology, School of Dental Medicine, University of Bern, Bern, Switzerland.|Laboratory of Oral Cell Biology, School of Dental University of Bern, Bern, Switzerland.
authors: R
authors: R
authors: Gruber
authors: Department of Periodontology, School of Dental Medicine, University of Bern, Freiburgstrasse 7, 3010, Bern, Switzerland. anton.sculean@zmk.unibe.ch.
authors: A
authors: A
authors: Sculean
mesh_terms: D000818:Animals; D001861:Bone Regeneration; D004285:Dogs; D008297:Male; D008903:Minerals
chemical_list: D008903:Minerals
references: Int J Periodontics Restorative Dent. 1998 Aug;18(4):321-31
references: 12693419
references: J Clin Periodontol. 2007 Jan;34(1):72-7
references: 17243997
references: Br J Oral Maxillofac Surg. 2011 Jan;49(1):37-41
references: 20106572
references: J Clin Periodontol. 2008 Sep;35(8 Suppl):106-16
references: 18724845
references: Clin Oral Implants Res. 2011 May;22(5):473-80
references: 21087317
references: Clin Oral Implants Res. 2006 Jun;17(3):237-43
references: 16672017
references: Int J Periodontics Restorative Dent. 2008 Apr;28(2):153-61
references: 18546811
references: J Clin Periodontol. 2004 Sep;31(9):770-6
references: 15312100
references: Int J Periodontics Restorative Dent. 2000 Feb;20(1):19-29
references: 11203545
references: Clin Oral Implants Res. 2012 Jan;23(1):95-9
references: 21554404
references: Clin Oral Implants Res. 2009 Oct;20(10):1099-104
references: 19681965
references: J Clin Periodontol. 2005 Jun;32(6):583-9
references: 15882215
references: Int J Periodontics Restorative Dent. 2005 Oct;25(5):509-18
references: 16250573
references: J Oral Sci. 2008 Sep;50(3):279-85
references: 18818463
references: J Periodontol. 2004 Feb;75(2):229-35
references: 15068110
references: Clin Oral Implants Res. 2007 Dec;18(6):752-60
references: 17888014
references: Clin Oral Implants Res. 2009 Oct;20(10):1124-32
references: 19508339
references: J Clin Periodontol. 2010 Jun;37(6):534-43
references: 20507377
references: J Clin Periodontol. 2006 Jul;33(7):491-9
references: 16820037
references: Int J Periodontics Restorative Dent. 2002 Aug;22(4):389-97
references: 12212686
references: J Clin Periodontol. 2005 Jul;32(7):720-4
references: 15966877
references: Int J Periodontics Restorative Dent. 2006 Apr;26(2):161-9
references: 16642905
references: J Clin Periodontol. 2003 Jan;30(1):73-80
references: 12702114
references: Clin Oral Implants Res. 2009 Oct;20(10):1116-23
references: 19719734
references: Drug Discov Today. 2003 Nov 1;8(21):980-9
references: 14643161
references: Clin Oral Investig. 2004 Jun;8(2):70-4
references: 14767696
references: Med Oral Patol Oral Cir Bucal. 2011 May 01;16(3):e448-53
references: 21196864
references: Clin Oral Investig. 2013 Mar;17(2):423-30
references: 22552592
references: J Biomed Mater Res B Appl Biomater. 2009 Jul;90(1):171-81
references: 19085941
references: Periodontol 2000. 1999 Feb;19:21-39
references: 10321214