title: Bmo-miR-9A down regulates the expression of Bm-ase gene in vitro.
abstract: MicroRNAs (miRNAs) are a class of non-protein coding small RNAs of 18-24 nucleotides in length that regulate expression of genes at post-transcriptional levels and play multiple roles in biological processes. Bm-ase plays an important role in the course of nerve development of the silkworm, Bombyx mori. Bmo-miR-9a is a conservative miRNA. By using target prediction software RNA22 and RNAhybrid, we found a target site of Bmo-miR-9a in the 3'UTR of Bm-ase gene. To verify the regulation function of Bmo-miR-9a on the expression of Bm-ase gene, a Bmo-miR-9a over-expressing vector and Bm-ase 3'UTR fused firefly luciferase gene reporter plasmid were constructed, respectively. Then they were used to co-transfect the BmN cells. The result showed that luciferase activity in the co-transfected cells was suppressed compared with the control. A similar result was obtained when BmN cells were co-transfected with artificial synthetic Bmo-miR-9a mimics and Bm-ase 3'UTR fused luciferase reporter plasmid. These results suggest that Bmo-miR-9a can down regulate the expression of Bm-ase gene.
authors: Jiangsu University of Science and Technology, Zhenjiang Jiangsu 212018, China.
authors: Fei
authors: F
authors: Song
authors: Yong C
authors: YC
authors: Huang
authors: Xin
authors: X
authors: Wang
authors: Shunming
authors: S
authors: Tang
authors: Xingjia
authors: X
authors: Shen
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics; D000818:Animals; D001483:Base Sequence; D012831:Bombyx / Q000201:enzymology / Q000235:genetics*; D017124:Conserved Sequence / Q000235:genetics*; D029721:Drosophila Proteins / Q000235:genetics; D035683:MicroRNAs / Q000235:genetics* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000235:genetics
chemical_list: D020413:3' Untranslated Regions; D029721:Drosophila Proteins; C547291:MIRN9 microRNA, Drosophila; D035683:MicroRNAs; D009419:Nerve Tissue Proteins; C407353:ase protein, Drosophila