title: [Genotoxicity risk assessment and oocytes: basis of genetic toxicology and application in reproductive science].
abstract: The aim of genotoxicity tests in germ cells is to assess the impact of exposure to environmental mutagens that may represent a risk for the fertility or for the offspring of exposed subject. The comet assay on mature mouse oocytes is a simple, reproductive and rapid test to study primary DNA damage in oocytes. This test is used to complete toxicology assays applied in first line to somatic cells, and could find many applications in reproductive toxicology to study impact of environmental factors on female germ cells. We describe a practical application of comet assay in reproductive biology to assess the genotoxicity of cryoprotectants used at high concentrations in oocyte vitrification protocols. This test allowed us to demonstrate that dimethylsulfoxide and ethylene glycol are non-genotoxic for the mouse oocytes and led us to hypothesize a genotoxic effect of 1,2-propanediol (PrOH) at high concentrations after having observed induction of significant DNA damage on CHO cell line and on mouse oocytes.
authors: FR CNRS 3098, ECCOREV, UMR CNRS 7263 AMU, IMBE, biogénotoxicologie, santé humaine & environnement, faculté de médecine, Aix-Marseille université, 27, boulevard Jean-Moulin, 13005 Marseille, France.
authors: A
authors: A
authors: Berthelot-Ricou
authors: J
authors: J
authors: Perrin
authors: T
authors: T
authors: Orsière
authors: M
authors: M
authors: Aye
authors: A
authors: A
authors: Roustan
authors: A
authors: A
authors: Botta
authors: B
authors: B
authors: Courbiere
mesh_terms: D000818:Animals; D016466:CHO Cells; D020552:Comet Assay / Q000379:methods; D003412:Cricetulus; D015925:Cryopreservation / Q000379:methods; D003451:Cryoprotective Agents / Q000008:administration & dosage / Q000633:toxicity*; D004249:DNA Damage / Q000187:drug effects; D005260:Female; D051379:Mice; D009152:Mutagenicity Tests; D009865:Oocytes / Q000737:chemistry*; D027724:Reproductive Techniques, Assisted / Q000009:adverse effects; D018570:Risk Assessment / Q000379:methods*
chemical_list: D003451:Cryoprotective Agents
keywords: Comet assay; Cryoprotectant; Cryoprotecteurs; DNA damage; Genotoxicity; Génotoxicité; Lésions de l’ADN; Oocyte; Ovocyte; Test des comètes