title: Antiangiogenic activity and pharmacogenomics of medicinal plants from traditional korean medicine.
abstract: Aim. In the present study, we investigated the antiangiogenic properties of 59 plants used in traditional Korean medicine. Selected phytochemicals were investigated in more detail for their modes of action. Methods. A modified chicken-chorioallantoic-membrane (CAM) assay using quail eggs was applied to test for antiangiogenic effects of plant extracts. A molecular docking in silico approached the binding of plant constituents to the vascular endothelial growth factor receptors 1 and 2 (VEGFR1, VEGFR2). Microarray-based mRNA expression profiling was employed to correlate the 50% inhibition concentrations (IC50) of a panel of 60 NCI cell lines to these phytochemicals. Results. Extracts from Acer mono leaves, Reynoutria sachalniensis fruits, Cinnamomum japonicum stems, Eurya japonica leaves, Adenophora racemosa whole plant, Caryopteris incana leaves-stems, and Schisandra chinensis stems inhibited angiogenesis more than 50% in quail eggs. Selected phytochemicals from Korean plants were analyzed in more detail using microarray-based mRNA expression profiles and molecular docking to VEGFR1 and VEGFR2. These results indicate multifactorial modes of action of these natural products. Conclusion. The antiangiogenic activity of plants used in traditional Korean medicine implicates their possible application for diseases where inhibition of blood vessel formation is desired, for example, cancer, macular degeneration, diabetic retinopathy and others.
authors: Department of Pharmaceutical Biology, Institute of Pharmacy and Biochemistry, Johannes Gutenberg University, Staudinger Weg 5, 55128 Mainz, Germany.
authors: Ean-Jeong
authors: EJ
authors: Seo
authors: Victor
authors: V
authors: Kuete
authors: Onat
authors: O
authors: Kadioglu
authors: Benjamin
authors: B
authors: Krusche
authors: Sven
authors: S
authors: Schröder
authors: Henry Johannes
authors: HJ
authors: Greten
authors: Joachim
authors: J
authors: Arend
authors: Ik-Soo
authors: IS
authors: Lee
authors: Thomas
authors: T
authors: Efferth
references: J Pathol. 2001 Sep;195(1):7-19
references: 11568887
references: Curr Diab Rep. 2012 Aug;12(4):355-63
references: 22638940
references: Phytomedicine. 2006;13 Suppl 5:122-9
references: 16772111
references: Oncol Rep. 2005 Mar;13(3):459-63
references: 15706417
references: Phytomedicine. 2009 Dec;16(12):1083-8
references: 19665361
references: Front Pharmacol. 2010 Oct 25;1:123
references: 21833168
references: Curr Oncol Rep. 2011 Apr;13(2):103-11
references: 21318618
references: Bull World Health Organ. 1985;63(6):965-81
references: 3879679
references: Curr Drug Targets. 2011 Jan;12(1):122-32
references: 20735354
references: Cancer Res. 2008 Jan 15;68(2):415-24
references: 18199535
references: Trends Mol Med. 2007 Aug;13(8):353-61
references: 17644431
references: PLoS One. 2012;7(5):e35584
references: 22590507
references: Eur J Cancer. 2011 Mar;47(4):508-14
references: 21185719
references: Biochem Pharmacol. 2010 Nov 1;80(9):1386-95
references: 20696143
references: Int J Cancer. 2006 Jun 1;118(11):2711-20
references: 16388516
references: Anticancer Res. 2004 May-Jun;24(3a):1545-51
references: 15274321
references: J Altern Complement Med. 2001;7 Suppl 1:S33-40
references: 11822633
references: Exp Cell Res. 2012 Aug 1;318(13):1586-96
references: 22522122
references: Planta Med. 2010 Aug;76(11):1143-54
references: 20486071
references: Cancer Lett. 2001 Sep 28;171(1):11-6
references: 11485823
references: Bioorg Med Chem. 2012 Jan 1;20(1):167-76
references: 22154557
references: FASEB J. 2005 Mar;19(3):342-53
references: 15746177
references: J Nat Prod. 2012 Mar 23;75(3):311-35
references: 22316239
references: FASEB J. 2001 Aug;15(10):1798-800
references: 11481234
references: Comb Chem High Throughput Screen. 2010 Dec;13(10):849-54
references: 20883187
references: J Heart Lung Transplant. 2004 Jun;23(6):666-73
references: 15366425
references: J Comput Chem. 2009 Dec;30(16):2785-91
references: 19399780
references: Br J Pharmacol Chemother. 1967 Mar;29(3):378-87
references: 6034385
references: Int J Oncol. 2003 Apr;22(4):741-50
references: 12632063
references: Biochem Pharmacol. 2004 Jul 1;68(1):3-10
references: 15183112
references: Curr Opin Investig Drugs. 2008 Jun;9(6):658-71
references: 18516765
references: Vascul Pharmacol. 2008 Apr-Jun;48(4-6):184-90
references: 18328790
references: Cancer Biother Radiopharm. 2008 Apr;23(2):222-8
references: 18454691
references: Nat Genet. 2000 Mar;24(3):236-44
references: 10700175
references: Expert Opin Ther Targets. 2013 Jan;17(1):7-20
references: 23121690
references: Target Oncol. 2009 Dec;4(4):297-305
references: 19876699
references: PLoS One. 2012;7(10):e44631
references: 23056181
references: Lloydia. 1974 Dec;37(4):621-32
references: 4615231
references: Angiogenesis. 2012 Sep;15(3):361-76
references: 22451299
references: Recent Pat Food Nutr Agric. 2011 Jan;3(1):30-48
references: 21114469
references: Cell Biochem Biophys. 2006;44(1):43-9
references: 16456233
references: Immunopharmacol Immunotoxicol. 2012 Oct;34(5):768-78
references: 22299827
references: Eur J Pharmacol. 2006 Dec 28;553(1-3):46-53
references: 17056031
references: Ochsner J. 2012 Spring;12(1):45-56
references: 22438782
references: Curr Drug Metab. 2011 Dec;12(10):989-96
references: 21892916
references: J Ethnopharmacol. 2011 Apr 12;134(3):803-12
references: 21291988
references: Planta Med. 2012 Jun;78(10):935-42
references: 22573366
references: Curr Med Chem. 2011;18(21):3136-55
references: 21671856
references: Phytomedicine. 2011 Mar 15;18(5):366-73
references: 20850954
references: Curr Drug Targets. 2011 Feb;12(2):173-81
references: 20887245
references: Cancer Lett. 2006 Jan 8;231(1):113-22
references: 16356836
references: Food Chem Toxicol. 2011 Jul;49(7):1626-32
references: 21515330
references: Naunyn Schmiedebergs Arch Pharmacol. 2003 Jan;367(1):56-67
references: 12616342
references: Pharmacogenomics J. 2006 Jul-Aug;6(4):269-78
references: 16432535
references: Hepatogastroenterology. 2010 Sep-Oct;57(102-103):1241-6
references: 21410066
references: Cancer Detect Prev. 2009;32(4):286-91
references: 19185431
references: Mol Pharmacol. 2005 Sep;68(3):635-43
references: 15947208
references: Curr Med Chem. 2007;14(19):2024-32
references: 17691944
references: Cancer Epidemiol Biomarkers Prev. 2005 Mar;14(3):596-604
references: 15767336
references: Mol Endocrinol. 2010 Jun;24(6):1287-96
references: 20375240
references: Mol Cancer Ther. 2008 Jan;7(1):152-61
references: 18202018
references: Mol Pharmacol. 2003 Nov;64(5):1029-36
references: 14573751
references: Am J Pathol. 2010 Jul;177(1):481-92
references: 20472894
references: Chin Med J (Engl). 2007 Oct 5;120(19):1710-5
references: 17935676
references: Curr Med Chem. 2012;19(33):5735-44
references: 23033949
references: J Ethnopharmacol. 2011 Sep 1;137(1):752-66
references: 21763411
references: J Exp Zool Suppl. 1987;1:325-8
references: 3110362
references: Science. 1997 Jan 17;275(5298):343-9
references: 8994024
references: Curr Drug Discov Technol. 2010 Mar;7(1):37-45
references: 20156142
references: Planta Med. 2010 Dec;76(17):2019-25
references: 20957595
references: Cancer Res. 2007 Dec 15;67(24):12007-17
references: 18089832
references: Evid Based Complement Alternat Med. 2013;2013:341724
references: 23935662
references: J Ethnopharmacol. 2012 Jun 1;141(2):557-70
references: 21963565
references: Phytomedicine. 2011 Aug 15;18(11):959-69
references: 21831619