title: Vero cells expressing porcine circovirus type 2-capsid protein and their diagnostic application.
abstract: Porcine circovirus type 2 (PCV2) is the causative agent of postweaning multisystemic wasting syndrome (PMWS) in swine. Although the incidences of PCV2-related diseases are ubiquitous throughout the world, the serological tools are rather limited, mainly because the virus does not induce any cytopathic effects in cells. The purpose of this study was to develop a rapid, sensitive and easy quantitative immunofluorescence assay (QIFA) using the recombinant PCV2 nucleocapsid protein (NCP) for the detection of PCV2-specific antibodies in pig sera. The recombinant PCV2 NCP was expressed in Vero cells by a lentivirus system. The performance of QIFA using these Vero cells as a diagnostic antigen was compared with currently available C-ELISA and I-ELISA; the relative sensitivity turned out to range from 92.5% up to 99.3%. The relative specificity was 93.3% when compared to C-ELISA as the gold standard. The serological experiment also indicated the inverse relationship between QIFA and the viral load in serum, semen, feces samples from 7 PCV2-positive boars. In addition, the PCV2 sequence detected from bone marrow cells shows 99% of sequence identity with PCV2 genome, confirming the infectivity of PCV2.
authors: Animal and Plant Quarantine Agency, MAFRA, Anyang 430-757, Republic of Korea; College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul 151-742, Republic of Korea.
authors: Yeon-Hee
authors: YH
authors: Kim
authors: Chang-Hee
authors: CH
authors: Kweon
authors: Seung-Won
authors: SW
authors: Kang
authors: Yoon I
authors: YI
authors: Oh
authors: Jae Young
authors: JY
authors: Song
authors: Kyoung Ki
authors: KK
authors: Lee
authors: Se Chang
authors: SC
authors: Park
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D036022:Capsid Proteins / Q000096:biosynthesis / Q000235:genetics / Q000302:isolation & purification; D002522:Chlorocebus aethiops; D017925:Circovirus / Q000276:immunology*; D003955:Diagnostic Tests, Routine / Q000379:methods*; D005455:Fluorescent Antibody Technique / Q000379:methods*; D053570:Porcine Postweaning Multisystemic Wasting Syndrome / Q000175:diagnosis*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics / Q000302:isolation & purification; D012680:Sensitivity and Specificity; D013552:Swine; D014709:Vero Cells; D014730:Veterinary Medicine / Q000379:methods*
chemical_list: D000914:Antibodies, Viral; D036022:Capsid Proteins; D011994:Recombinant Proteins
keywords: Boar; Lentiviral transduction; Porcine circovirus type 2; Quantitative IFA