title: Quantitative proteomics reveals oxygen-dependent changes in neuronal mitochondria affecting function and sensitivity to rotenone.
abstract: Mitochondria are implicated in a variety of degenerative disorders and aging. Mitochondria are responsive to the oxygen in their environment, yet tissue culture is performed at atmospheric (21%) oxygen and not at physiological (1-11%) oxygen levels found in tissues. We employed imaging of mitochondrial probes, mass spectrometry, Western blots, and ATP assays of the human neuroblastoma cell-line SH-SY5Y and imaging of mitochondrial probes in human primary neurons under standard nonphysiological oxygen conditions (atmospheric) and under physiological oxygen levels in the nervous system to assess the impact of oxygen on mitochondrial function. SH-SY5Y cells cultured in physiological 5% oxygen exhibited the lowest reactive oxygen species (ROS) production, indicating that culture at 5% oxygen is favored; these results were mimicked in primary human cells. Mass spectrometric analysis revealed extensive mitochondrial proteomic alterations in SH-SY5Y cells based on oxygen culture condition. Among these, the rotenone-sensitive subunit of complex I NDUFV3 was increased in cells cultured at 5% oxygen. Rotenone is a Parkinson's disease-linked toxin, and correspondingly SH-SY5Y cells cultured at 5% oxygen also exhibited over 10 times greater sensitivity to rotenone than those cultured in atmospheric, 21%, oxygen. Our results indicate that neuronal mitochondria are responsive to oxygen levels and produce differential responses under different oxygen levels.
authors: Department of Pharmacology and Experimental Neuroscience, University of Nebraska Medical Center , 985800 Nebraska Medical Center, Omaha, Nebraska 68198, United States.
authors: Lance
authors: L
authors: Villeneuve
authors: Leann M
authors: LM
authors: Tiede
authors: Brenda
authors: B
authors: Morsey
authors: Howard S
authors: HS
authors: Fox
mesh_terms: D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D045222:Electron Transport Chain Complex Proteins / Q000378:metabolism; D006801:Humans; D020128:Inhibitory Concentration 50; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism*; D009474:Neurons / Q000187:drug effects / Q000378:metabolism*; D010100:Oxygen / Q000378:metabolism*; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics; D017382:Reactive Oxygen Species / Q000378:metabolism; D012402:Rotenone / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000378:metabolism; D014475:Uncoupling Agents / Q000494:pharmacology*; D000099156:Superoxide Dismutase 2
chemical_list: D045222:Electron Transport Chain Complex Proteins; D010100:Oxygen; D020543:Proteome; D017382:Reactive Oxygen Species; D012402:Rotenone; D013482:Superoxide Dismutase; D014475:Uncoupling Agents; D000099156:Superoxide Dismutase 2
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