title: Lysophosphatidylcholine-induced cytotoxicity in osteoblast-like MG-63 cells: involvement of transient receptor potential vanilloid 2 (TRPV2) channels.
abstract: Epidemiological studies indicate that patients suffering from atherosclerosis are predisposed to develop osteoporosis. Accordingly, atherogenic determinants such as oxidized low density lipoprotein (OxLDL) particles have been shown to alter bone cell functions. In this work, we investigated the cytotoxicity of lysophosphatidylcholine (lysoPC), a major phospholipid component generated upon LDL oxidation, on bone-forming MG-63 osteoblast-like cells. Cell viability was reduced by lysoPC in a concentration-dependent manner with a LC50 of 18.7±0.7 μM. LysoPC-induced cell death was attributed to induction of both apoptosis and necrosis. Since impairment of intracellular calcium homeostasis is often involved in mechanism of cell death, we determined the involvement of calcium in lysoPC-induced cytotoxicity. LysoPC promoted a rapid and transient increase in intracellular calcium attributed to mobilization from calcium stores, followed by a sustained influx. Intracellular calcium mobilization was associated to phospholipase C (PLC)-dependent mobilization of calcium from the endoplasmic reticulum since inhibition of PLC or calcium depletion of reticulum endoplasmic with thapsigargin prevented the calcium mobilization. The calcium influx induced by lysoPC was abolished by inhibition of transient receptor potential vanilloid (TRPV) channels with ruthenium red whereas gadolinium, which inhibits canonical TRP (TRPC) channels, was without effect. Accordingly, expression of TRPV2 and TRPV4 were shown in MG-63 cells. The addition of TRPV2 inhibitor Tranilast in the incubation medium prevent the calcium influx triggered by lysoPC and reduced lysoPC-induced cytotoxicity whereas TRPV4 inhibitor RN 1734 was without effect, which confirms the involvement of TRPV2 activation in lysoPC-induced cell death.
authors: Laboratoire du Métabolisme Osseux, BIOMED, Département des Sciences Biologiques, Université du Québec à Montréal, Montreal, Quebec, Canada.
authors: Abdallah
authors: A
authors: Fallah
authors: Rachel
authors: R
authors: Pierre
authors: Elie
authors: E
authors: Abed
authors: Robert
authors: R
authors: Moreau
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D002118:Calcium / Q000378:metabolism*; D016923:Cell Death / Q000187:drug effects*; D006801:Humans; D008244:Lysophosphatidylcholines / Q000494:pharmacology*; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects; D015398:Signal Transduction / Q000187:drug effects; D013449:Sulfonamides / Q000494:pharmacology; D050916:TRPV Cation Channels / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D010738:Type C Phospholipases / Q000378:metabolism; D062367:ortho-Aminobenzoates / Q000494:pharmacology
chemical_list: D008244:Lysophosphatidylcholines; C544743:RN 1734; D013449:Sulfonamides; D050916:TRPV Cation Channels; C400769:TRPV2 protein, human; C416986:TRPV4 protein, human; D062367:ortho-Aminobenzoates; D010738:Type C Phospholipases; C012293:tranilast; D002118:Calcium