title: Effect of castration on renal glycosaminoglycans and their urinary excretion in male and female rats with chronic renal failure.
abstract: Glycosaminoglycans (GAGs) participate in a variety of processes in the kidney, and evidence suggests that gender-related hormones participate in renal function. The aim of this study was to analyze the relationship of GAGs, gender, and proteinuria in male and female rats with chronic renal failure (CRF). GAGs were analyzed in total kidney tissue and 24-h urine of castrated (c), male (M), and female (F) Wistar control (C) rats (CM, CMc, CF, CFc) and after 30 days of CRF induced by 5/6 nephrectomy (CRFM, CRFMc, CRFF, CRFFc). Total GAG quantification and composition were determined using agarose and polyacrylamide gel electrophoresis, respectively. Renal GAGs were higher in CF compared to CM. CRFM presented an increase in renal GAGs, heparan sulfate (HS), and proteinuria, while castration reduced these parameters. However, CRFF and CRFFc groups showed a decrease in renal GAGs concomitant with an increase in proteinuria. Our results suggest that, in CRFM, sex hormones quantitatively alter GAGs, mainly HS, and possibly the glomerular filtration barrier, leading to proteinuria. The lack of this response in CRFMc, where HS did not increase, corroborates this theory. This pattern was not observed in females. Further studies of CRF are needed to clarify gender-dependent differences in HS synthesis.
authors: Universidade do Estado do Rio de Janeiro, Disciplina de Nefrologia, Faculdade de Ciências Médicas, Rio de JaneiroRJ, Brasil. lemoscc@hotmail.com
authors: C C S
authors: CC
authors: Lemos
authors: A M F
authors: AM
authors: Tovar
authors: M A M
authors: MA
authors: Guimarães
authors: R
authors: R
authors: Bregman
mesh_terms: D000818:Animals; D002369:Castration; D004587:Electrophoresis, Agar Gel; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005919:Glomerular Filtration Rate; D006025:Glycosaminoglycans / Q000302:isolation & purification / Q000652:urine*; D012739:Gonadal Steroid Hormones / Q000172:deficiency*; D006497:Heparan Sulfate / Q000652:urine; D007668:Kidney / Q000737:chemistry* / Q000601:surgery; D007676:Kidney Failure, Chronic / Q000378:metabolism* / Q000601:surgery; D008297:Male; D009392:Nephrectomy; D011507:Proteinuria / Q000652:urine*; D011897:Random Allocation; D017208:Rats, Wistar; D012737:Sex Factors
chemical_list: D006025:Glycosaminoglycans; D012739:Gonadal Steroid Hormones; D006497:Heparan Sulfate
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