title: Androgen receptor increases CD133 expression and progenitor-like population that associate with cisplatin resistance in endometrial cancer cell line.
abstract: Endometrial cancer (EMC) is a sex steroid hormone-related female malignancy. Androgen and androgen receptor (androgen/AR) signals have been implicated in EMC progression. Cancer stem/progenitor cells (CSPCs) are suspected to link to chemoresistance in patients with EMC. In this study, we examined the androgen/AR roles in cisplatin resistance and CSPC population. We found AR expression increased naive EMC side population, CSPC population, cell migration, and epithelial-mesenchymal transition. Meanwhile, it decreased cisplatin cytotoxic effect on EMC cells. Collaterally, endogenous AR expressions in EMC cells were upregulated in the cisplatin-resisting state. Moreover, AR expression could further enhance CD133 expression, CSPC-related markers, and drug-resistance gene messenger RNA expression in EMC cells. Finally, the AR-associated gene expression might go through indirect regulation. This is the first report revealing AR function on EMC cells' CSPC and cisplatin resistance.
authors: 1Sex Hormone Research Center, Graduate Institution of Clinical Medical Science, School of Medicine, China Medical University, Taichung, Taiwan.
authors: Lumin
authors: L
authors: Chen
authors: Wei-Chun
authors: WC
authors: Chang
authors: Yao-Ching
authors: YC
authors: Hung
authors: Ying-Yi
authors: YY
authors: Chang
authors: Bo-Yin
authors: BY
authors: Bao
authors: Hsin-Ching
authors: HC
authors: Huang
authors: Wei-Min
authors: WM
authors: Chung
authors: Chih-Rong
authors: CR
authors: Shyr
authors: Wen-Lung
authors: WL
authors: Ma
mesh_terms: D000071916:AC133 Antigen; D015703:Antigens, CD / Q000096:biosynthesis*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects / Q000502:physiology; D002945:Cisplatin / Q000494:pharmacology / Q000627:therapeutic use*; D019008:Drug Resistance, Neoplasm / Q000187:drug effects / Q000502:physiology*; D016889:Endometrial Neoplasms / Q000188:drug therapy / Q000378:metabolism*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006023:Glycoproteins / Q000096:biosynthesis*; D006801:Humans; D014411:Neoplastic Stem Cells / Q000187:drug effects / Q000378:metabolism*; D010455:Peptides; D011944:Receptors, Androgen / Q000096:biosynthesis*
chemical_list: D000071916:AC133 Antigen; C410798:AR protein, human; D015703:Antigens, CD; D006023:Glycoproteins; C000606023:PROM1 protein, human; D010455:Peptides; D011944:Receptors, Androgen; D002945:Cisplatin
keywords: AR; cancer stem/progenitor cells; cisplatin resistance; endometrial cancer; side population cell
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