title: HLA-A, -B, -DRB1 allele and haplotype frequencies and comparison with blood group antigens in dialysis patients in the East Anatolia region of Turkey.
abstract: OBJECTIVE
  The first aim of that study was to investigate HLA class I and class II allele and haplotype frequencies in renal dialysis patients who live in East Anatolia in Turkey. Our second aim was to investigate whether there was a relationship between ABO and D blood group antigens and HLA alleles and haplotypes for the study group.
  
  
  METHODS
  HLA class I and II polymorphisms in 408 renal dialysis patients were studied using sequence-specific primers (SSP) and sequence-specific oligonucleotides (SSO). Blood group antigens were detected by agglutination methods on microplates.
  
  
  RESULTS
  A total of 16 HLA-A, 34 HLA-B, and 15 HLA-DRB1 alleles were identified. The most frequent HLA-A alleles were HLA-A*02, HLA-A*24, and HLA-A*11. The most frequent HLA-B alleles were HLA-B*35, HLA-B*51, and HLA-B*44. In case of HLA-DRB1; HLA-DRB1*11, HLA-DRB1*04, and HLA-DRB1*13 were first 3 alleles with higher frequency, in order. In the combination of those 3 alleles, the most frequent HLA-A-B-DRB1 haplotypes were HLA-A*02-B*51-DRB1*11, HLA-A*11-B*35-DRB1*11, A*24-B*35-DRB1*11. The frequency of ABO, D blood group antigens were observed as 0.168 for A Rh(+), 0.019 for A Rh(-), 0.057 for B Rh(+), 0.013 for B Rh(-), 0.123 for O Rh(+), 0.014 for O Rh(-), 0.018 for AB Rh(+), and 0.001 for AB Rh(-). While A Rh(+) samples with HLA-A*02 and HLA-DRB1*11 had the highest frequencies (0.067 and 0.088, respectively), O Rh(+) samples with HLA-B*51 had the highest frequency (0.06).
  
  
  CONCLUSIONS
  According to haplotype frequencies HLA-A*02-B*51-DRB1*11 is also found at higher frequencies in Bulgarian and Armenian populations. In case of HLA-associated diseases, the east Anatolian population could be susceptible to myastenia gravis, Behçet's disease, and systemic sclerosis due to the high frequencies of HLA-A*24, HLA-B*51, and HLA-DRB1*11 respectively. We did not observe a correlation between blood group antigens and HLA alleles or haplotypes in renal dialysis patients.
authors: Department of Medical Biology and Genetics, Inonu University Faculty of Medicine, Turgut Ozal Medical Center, Malatya, Turkey. kayhanbasak@hotmail.com
authors: B
authors: B
authors: Kayhan
authors: E L
authors: EL
authors: Kurtoglu
authors: H
authors: H
authors: Taskapan
authors: T
authors: T
authors: Piskin
authors: I
authors: I
authors: Sahin
authors: G
authors: G
authors: Otlu
authors: B
authors: B
authors: Unal
mesh_terms: D000017:ABO Blood-Group System / Q000097:blood*; D015415:Biomarkers / Q000097:blood; D005260:Female; D005787:Gene Frequency; D015234:HLA-A Antigens / Q000235:genetics*; D015235:HLA-B Antigens / Q000235:genetics*; D059811:HLA-DRB1 Chains / Q000235:genetics*; D006239:Haplotypes; D006785:Hospitals, University; D006801:Humans; D008297:Male; D010641:Phenotype; D011110:Polymorphism, Genetic; D006435:Renal Dialysis; D051436:Renal Insufficiency, Chronic / Q000097:blood / Q000175:diagnosis / Q000208:ethnology / Q000235:genetics / Q000276:immunology / Q000628:therapy*; D012204:Rh-Hr Blood-Group System / Q000097:blood*; D014421:Turkey / Q000453:epidemiology
chemical_list: D000017:ABO Blood-Group System; D015415:Biomarkers; D015234:HLA-A Antigens; D015235:HLA-B Antigens; D059811:HLA-DRB1 Chains; D012204:Rh-Hr Blood-Group System