title: Simultaneous determination of 10 nucleosides and nucleobases in Antrodia camphorata using QTRAP LC-MS/MS.
abstract: A liquid chromatography-triple-quadrupole linear ion trap mass spectrometry (LC-QTrap-MS) analysis has been developed for the identiﬁcation and quantiﬁcation of 10 nucleosides and nucleobases in extracts of Antrodia camphorata. The method was successfully used to qualitatively identify for six nucleosides namely, cytidine, uridine, inosine, guanosine, thymidine, adenosine and four nucleobases namely, uracil, guanine, xanthine, adenine in A. camphorata. Under optimized chromatographic conditions, good separation for 10 target compounds were obtained on an Agilent HC-C18(2) column (4.6 × 250 mm, 5 μm) eluted by a mobile phase of 5 mM ammonium acetate solution-methanol at a flow rate of 0.5 mL/min. Data acquisition was carried out in multiple reaction monitoring transition mode. Additional identiﬁcation and conﬁrmation of target compounds were performed using the enhanced product ion modus of the linear ion trap. It was the first report about simultaneous analysis of nucleosides and nucleobases in A. camphorata using this method. These results demonstrated that the QTRAP LC-MS/MS was a useful tool for quality evaluation of some medicinal plant products by using nucleosides and nucleobases as chemical markers. This method might also be utilized for the investigation of edible plant materials and agricultural products containing nucleosides and nucleobases.
authors: Department of Analysis of Chinese Medicine, Nanjing University of Chinese Medicine, Nanjing 210023, PR China.
authors: Fei
authors: F
authors: Chen
authors: Department of Analysis of Chinese Medicine, Nanjing University of Chinese Medicine, Nanjing 210023, PR China.
authors: Fengsu
authors: F
authors: Zhang
authors: Department of Analysis of Chinese Medicine, Nanjing University of Chinese Medicine, Nanjing 210023, PR China.
authors: Nianyun
authors: N
authors: Yang
authors: Department of Analysis of Chinese Medicine, Nanjing University of Chinese Medicine, Nanjing 210023, PR China liuxunh1959@sohu.com.
authors: Xunhong
authors: X
authors: Liu
mesh_terms: D000225:Adenine / Q000032:analysis*; D055491:Antrodia / Q000737:chemistry*; D002853:Chromatography, Liquid / Q000379:methods*; D006147:Guanine / Q000032:analysis*; D009705:Nucleosides / Q000032:analysis*; D010936:Plant Extracts / Q000032:analysis; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014498:Uracil / Q000032:analysis*; D019820:Xanthine / Q000032:analysis*
chemical_list: D009705:Nucleosides; D010936:Plant Extracts; D019820:Xanthine; D014498:Uracil; D006147:Guanine; D000225:Adenine