title: In vitro cultivation model for Heterosporis saurida (Microsporidia) isolated from lizardfish, Saurida undosquamis (Richardson).
abstract: Heterosporis saurida is a microsporidian that infects lizardfish, Saurida undosquamis (Richardson, 1848), in the Arabian Sea. Spores were isolated from infected lizardfish and used to infect derived fish cell lines: common carp brain (CCB), epithelioma papulosum cyprinid (EPC), fathead minnow epithelial (FHM), rainbow trout gonad (RTG), bluegill fry (BF-2) and chinook salmon embryo (CHSE). Non-fish cell lines were also tested that include: insect (SF-9), rabbit (RK-13) and African green monkey (Vero E6). No growth of H. saurida was observed in any fish cell line, SF-9 or Vero E6 cell lines. H. saurida spores grew only in RK-13 cell line and were detected by immunofluorescence. Developmental stages of H. saurida were seen in RK-13 cells by light and transmission electron microscopy, and species identification was confirmed by sequencing. This study demonstrated that H. saurida was able to proliferate in the mammalian RK-13 cell line, which thus represents an in vitro model for conducting molecular genetics and cell-pathogen interaction studies of Heterosporis.
authors: Clinical Division of Fish Medicine, University of Veterinary Medicine, Vienna, Austria.
authors: G
authors: G
authors: Kumar
authors: M
authors: M
authors: Saleh
authors: A-A S
authors: AA
authors: Abdel-Baki
authors: S
authors: S
authors: Al-Quraishy
authors: M
authors: M
authors: El-Matbouli
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques / Q000662:veterinary; D005393:Fish Diseases / Q000469:parasitology*; D016814:Microsporidia / Q000235:genetics / Q000302:isolation & purification*; D016881:Microsporidiosis / Q000469:parasitology / Q000662:veterinary*; D016133:Polymerase Chain Reaction / Q000662:veterinary
keywords: Heterosporis saurida; cell lines; lizardfish; microsporidia; rabbit kidney cell line