title: Development of a multi-residue method for fast screening and confirmation of 20 prohibited veterinary drugs in feedstuffs by liquid chromatography tandem mass spectrometry.
abstract: A simple multiresidue method was developed for detecting and quantifying twenty analytes from 5 classes of prohibited veterinary drugs (β-agonists (9), anabolic hormones (4), quinoxalines (4), tranquilizers (1), cyproheptadine, and clonidine in animal feeds using a QuEChERS (Quick, Easy, Cheap, Effective, Rugged and Safe) approach. Feed samples were extracted by ultrasonic-assisted extraction with a mixture of methanol-acetonitrile (50:50, v/v), followed by a cleanup using a dispersive solid-phase extraction with PSA (primary secondary amine). Target compounds were separated and determined by a liquid chromatography tandem quadrupole mass spectrometer operating in positive electrospray ionization mode, using multiple reaction monitoring (MRM). The recoveries of these compounds were between 56.7% and 103% at three spiked levels. The repeatability was lower than 10%, whereas reproducibility was no more than 15% except for nandrolone (17% at 10μgkg(-1)) and diazepam (19% at 10μgkg(-1)). Decision limits (CCαs) and detection capabilities (CCβs) ranged from 0.42 to 5.74μgkg(-1) and 5.70-9.81μgkg(-1), respectively. The method was successfully applied to screening of real samples obtained from local feed markets and confirmation of the suspected target analytes.
authors: National Reference Laboratory of Veterinary Drug Residues (SCAU), College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China.
authors: Gui-Jun
authors: GJ
authors: Zhang
authors: Bing-Hu
authors: BH
authors: Fang
authors: Ya-Hong
authors: YH
authors: Liu
authors: Xu-Feng
authors: XF
authors: Wang
authors: Li-Xiao
authors: LX
authors: Xu
authors: Ya-Ping
authors: YP
authors: Zhang
authors: Li-Min
authors: LM
authors: He
mesh_terms: D000821:Animal Feed / Q000032:analysis* / Q000592:standards; D002853:Chromatography, Liquid / Q000379:methods*; D057230:Limit of Detection; D016014:Linear Models; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry / Q000379:methods*; D019155:Veterinary Drugs / Q000032:analysis* / Q000737:chemistry
chemical_list: D019155:Veterinary Drugs
keywords: Feed; Liquid chromatography–tandem mass spectrometry; Multiclass drugs; QuEChERS