title: Identification and determination of antibiotic susceptibilities of Brucella strains isolated from patients in van, Turkey by conventional and molecular methods.
abstract: OBJECTIVE
  Brucellosis is a worldwide zoonotic disease and still constitutes a major public health problem. In this study, we aimed to identify biovars of Brucella strains isolated from clinical specimens taken from brucellosis patients from the Eastern Anatolia region as well determine the susceptibility of these isolates to tigecycline and azithromycin, drugs that may serve as alternatives to the conventional drugs used in the therapy.
  
  
  METHODS
  Seventy-five Brucella spp. isolates were included in the study. All strains were identified by both conventional and molecular methods. Brucella Multiplex PCR kit (FC-Biotech, Code: 0301, Turkey) and B. melitensis biovar typing PCR kit (FC-Biotech, Code: 0302, Turkey) were used for molecular typing. Antimicrobial susceptibilities of all strains were determined by E-tests.
  
  
  RESULTS
  By conventional biotyping, 73 strains were identified as B. melitensis biovar 3 and two strains as B. abortus biovar 3. Molecular typing results were compatible with conventional methods. The MIC50 and MIC90 values of doxycycline were 0.047 and 0.094; tigecycline 0.094 and 0.125; trimethoprim/sulfamethoxazole 0.064 and 0.19; ciprofloxacin 0.19 for both; streptomycin 0.75 and 1; rifampin 1 and 2 and azithromycin 4 and 8. According to the MIC values, doxycycline was found to be the most effective antibiotic, followed by tigecycline, trimethoprim-sulfamethoxazole and ciprofloxacin.
  
  
  CONCLUSIONS
  Currently recommended antibiotics for the treatment of brucellosis such as doxycycline, rifampin, streptomycin, trimethoprim-sulfamethoxazole and ciprofloxacin were found to be still effective. While our results showed that tigecycline can be used an alternative agent in the treatment of brucellosis, azithromycin has not been confirmed as an appropriate agent for the treatment.
authors: Microbiology Laboratory, Van Education and Training Hospital, Van, Turkey.
authors: Mehmet
authors: M
authors: Parlak
authors: Hüseyin
authors: H
authors: Güdücüoğlu
authors: Yasemin
authors: Y
authors: Bayram
authors: Aytekin
authors: A
authors: Çıkman
authors: Cenk
authors: C
authors: Aypak
authors: Selçuk
authors: S
authors: Kılıç
authors: Mustafa
authors: M
authors: Berktaş
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002002:Brucella / Q000145:classification / Q000187:drug effects* / Q000472:pathogenicity; D006801:Humans; D008826:Microbial Sensitivity Tests; D014421:Turkey
chemical_list: D000900:Anti-Bacterial Agents
keywords: Brucella; E-test.; antibiotic sensitivity; biotyping
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