title: Postmortem stability of S100B in the aqueous humor of northern fur seals (Callorhinus ursinus).
abstract: Bycatch (accidental drowning in fishing nets) is a significant problem for some marine mammal species, but can be difficult to diagnose as there are no pathognomonic gross or histological lesions. In human medicine, biomarkers such as S100B are increasingly being used to investigate hypoxic-ischemic syndromes, but, to the authors' knowledge, studies using this marker have not been reported for marine mammal species. The aims of the current study were to determine baseline postmortem S100B levels in a pinniped species, and to determine whether S100B levels were stable over a postmortem interval of 48 hr. Aqueous humor, which is simple to collect and avoids many of the problems associated with postmortem collection of blood, was used as a surrogate for serum. S100B was detected in the aqueous humor of acute deaths (<15 min) and was stable for up to 48 hr, with a wider variation in values at the 48-hr time interval.
authors: 1Wendi D. Roe, Pathobiology Group, Institute of Veterinary Animal and Biomedical Sciences, Massey University, Private Bag 11222, Tennant Drive, Palmerston North PN4442, New Zealand. w.d.roe@massey.ac.nz.
authors: Wendi D
authors: WD
authors: Roe
authors: Terry R
authors: TR
authors: Spraker
authors: Colleen G
authors: CG
authors: Duncan
authors: Marjorie
authors: M
authors: Owen
authors: J Brad
authors: JB
authors: Charles
mesh_terms: D000818:Animals; D001082:Aqueous Humor / Q000737:chemistry*; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D046069:Fur Seals; D020925:Hypoxia-Ischemia, Brain / Q000175:diagnosis / Q000662:veterinary*; D008297:Male; D064568:S100 Calcium Binding Protein beta Subunit / Q000032:analysis*
chemical_list: D064568:S100 Calcium Binding Protein beta Subunit
keywords: Biomarker; S100B; bycatch; drowning; ischemia; pinniped; seals