title: Targeting single-walled carbon nanotubes for the treatment of breast cancer using photothermal therapy.
abstract: This paper focuses on the targeting of single-walled carbon nanotubes (SWNTs) for the treatment of breast cancer with minimal side effects using photothermal therapy. The human protein annexin V (AV) binds specifically to anionic phospholipids expressed externally on the surface of tumour cells and endothelial cells that line the tumour vasculature. A 2 h incubation of the SWNT-AV conjugate with proliferating endothelial cells followed by washing and near-infrared (NIR) irradiation at a wavelength of 980 nm was enough to induce significant cell death; there was no significant cell death with irradiation or the conjugate alone. Administration of the same conjugate i.v. in BALB/c female mice with implanted 4T1 murine mammary at a dose of 0.8 mg SWNT kg(-1) and followed one day later by NIR irradiation of the tumour at a wavelength of 980 nm led to complete disappearance of implanted 4T1 mouse mammary tumours for the majority of the animals by 11 days since the irradiation. The combination of the photothermal therapy with the immunoadjuvant cyclophosphamide resulted in increased survival. The in vivo results suggest the SWNT-AV/NIR treatment is a promising approach to treat breast cancer.
authors: Bioengineering Center, University of Oklahoma, 202 W. Boyd, Norman, OK 73019, USA.
authors: Luís F F
authors: LF
authors: Neves
authors: John J
authors: JJ
authors: Krais
authors: Brent D
authors: BD
authors: Van Rite
authors: Rajagopal
authors: R
authors: Ramesh
authors: Daniel E
authors: DE
authors: Resasco
authors: Roger G
authors: RG
authors: Harrison
mesh_terms: D000818:Animals; D017304:Annexin A5 / Q000302:isolation & purification / Q000378:metabolism; D019921:Biotinylation; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002470:Cell Survival; D042783:Endothelial Cells / Q000166:cytology / Q000378:metabolism; D005260:Female; D016650:Fluorescein-5-isothiocyanate / Q000378:metabolism; D006801:Humans; D006979:Hyperthermia, Induced; D008175:Lung Neoplasms / Q000473:pathology / Q000556:secondary; D008301:Maleimides / Q000737:chemistry; D015674:Mammary Neoplasms, Animal / Q000473:pathology / Q000628:therapy*; D051379:Mice; D008807:Mice, Inbred BALB C; D018625:Microscopy, Atomic Force; D008856:Microscopy, Fluorescence; D037742:Nanotubes, Carbon / Q000737:chemistry*; D010714:Phosphatidylethanolamines / Q000737:chemistry; D010789:Phototherapy; D011092:Polyethylene Glycols / Q000737:chemistry; D011994:Recombinant Proteins / Q000302:isolation & purification; D019265:Spectroscopy, Near-Infrared; D013194:Staining and Labeling; D013535:Suspensions
chemical_list: D017304:Annexin A5; D008301:Maleimides; D037742:Nanotubes, Carbon; D010714:Phosphatidylethanolamines; D011994:Recombinant Proteins; D013535:Suspensions; C038089:1,2-distearoylphosphatidylethanolamine; C043592:maleimide; D011092:Polyethylene Glycols; D016650:Fluorescein-5-isothiocyanate