title: Micro- and nanofluidic technologies for epigenetic profiling.
abstract: This short review provides an overview of the impact micro- and nanotechnologies can make in studying epigenetic structures. The importance of mapping histone modifications on chromatin prompts us to highlight the complexities and challenges associated with histone mapping, as compared to DNA sequencing. First, the histone code comprised over 30 variations, compared to 4 nucleotides for DNA. Second, whereas DNA can be amplified using polymerase chain reaction, chromatin cannot be amplified, creating challenges in obtaining sufficient material for analysis. Third, while every person has only a single genome, there exist multiple epigenomes in cells of different types and origins. Finally, we summarize existing technologies for performing these types of analyses. Although there are still relatively few examples of micro- and nanofluidic technologies for chromatin analysis, the unique advantages of using such technologies to address inherent challenges in epigenetic studies, such as limited sample material, complex readouts, and the need for high-content screens, make this an area of significant growth and opportunity.
authors: Department of Biomedical Engineering, University of Michigan, Ann Arbor, Michigan 48109, USA.
authors: Toshiki
authors: T
authors: Matsuoka
authors: Byoung
authors: B
authors: Choul Kim
authors: Christopher
authors: C
authors: Moraes
authors: Minsub
authors: M
authors: Han
authors: Shuichi
authors: S
authors: Takayama
references: Nat Rev Cancer. 2004 Feb;4(2):143-53
references: 14732866
references: Nat Chem Biol. 2012 Apr 17;8(5):417-27
references: 22510649
references: Nat Cell Biol. 2011 May;13(5):497-505
references: 21540845
references: Nat Struct Mol Biol. 2013 Mar;20(3):259-66
references: 23463310
references: Dev Dyn. 2007 Dec;236(12):3199-207
references: 17685475
references: Cell. 2002 Feb 22;108(4):475-87
references: 11909519
references: Biophys J. 2009 Dec 16;97(12):3150-7
references: 20006952
references: Genes Dev. 2006 Apr 15;20(8):966-76
references: 16598039
references: Proc Natl Acad Sci U S A. 2006 Nov 21;103(47):17690-5
references: 17101994
references: Semin Reprod Med. 2009 Sep;27(5):403-8
references: 19711250
references: Proc Natl Acad Sci U S A. 1981 Mar;78(3):1461-5
references: 6940168
references: Nat Biotechnol. 2012 Aug;30(8):771-6
references: 22797562
references: Proc Natl Acad Sci U S A. 2012 May 29;109(22):8477-82
references: 22586076
references: Biophys J. 2009 Mar 18;96(6):2082-94
references: 19289035
references: Proc Natl Acad Sci U S A. 2009 Aug 11;106(32):13317-22
references: 19651606
references: Methods. 1999 Nov;19(3):425-33
references: 10579938
references: Nature. 2012 Sep 6;489(7414):52-5
references: 22955614
references: Lab Chip. 2009 Oct 7;9(19):2772-4
references: 19967112
references: Anal Bioanal Chem. 2008 Aug;391(7):2395-409
references: 18340435
references: Stem Cells. 2006 Mar;24(3):501-5
references: 16293574
references: Cell. 2013 Mar 14;152(6):1324-43
references: 23498940
references: Cell. 2006 Aug 25;126(4):663-76
references: 16904174
references: Nucleic Acids Res. 2008 Feb;36(3):e17
references: 18203739
references: Genes Dev. 2010 Jul 15;24(14):1465-78
references: 20634313
references: Science. 2001 Apr 6;292(5514):110-3
references: 11283354
references: Trends Genet. 2013 Mar;29(3):176-86
references: 23410786
references: Proc Natl Acad Sci U S A. 2000 Jan 4;97(1):127-32
references: 10618382
references: Cell. 2012 Mar 16;148(6):1123-31
references: 22424224
references: Acta Psychiatr Scand. 2013 Jul;128(1):97
references: 23331212
references: Proc Natl Acad Sci U S A. 1976 Jun;73(6):1897-901
references: 1064861
references: EMBO J. 2012 Apr 4;31(7):1644-53
references: 22343941
references: Cell. 2012 Mar 16;148(6):1110-22
references: 22424223
references: Neurobiol Aging. 2010 Dec;31(12):2025-37
references: 19117641
references: Nat Struct Mol Biol. 2009 May;16(5):534-40
references: 19377481
references: Nat Genet. 2006 Jul;38(7):835-41
references: 16767102
references: Trends Biochem Sci. 2011 Jan;36(1):1-6
references: 20926298
references: Phys Rev Lett. 2005 May 20;94(19):196101
references: 16090189
references: PLoS One. 2010 Sep 30;5(9):
references: 20927350
references: Neuropsychopharmacology. 2013 Jan;38(1):220-36
references: 22781843
references: Nano Lett. 2012 Dec 12;12(12):6480-4
references: 23186544
references: Rejuvenation Res. 2004 Spring;7(1):15-31
references: 15256042
references: Nature. 2013 Mar 14;495(7440):193-8
references: 23467092
references: J Biol Chem. 2012 Feb 17;287(8):5183-91
references: 22157002
references: Biochem Biophys Res Commun. 1973 Sep 5;54(1):297-300
references: 4741568
references: Lab Chip. 2009 May 21;9(10):1365-70
references: 19417902
references: Nat Struct Mol Biol. 2007 Nov;14(11):1017-24
references: 17984964
references: Nat Rev Mol Cell Biol. 2012 Jun 22;13(7):436-47
references: 22722606
references: Chem Soc Rev. 2011 Jul;40(7):3677-702
references: 21442106
references: Cell. 2007 Feb 23;128(4):683-92
references: 17320506
references: PLoS One. 2009 Dec 03;4(12):e8152
references: 19997644
references: Curr Opin Cell Biol. 2013 Apr;25(2):184-9
references: 23347561
references: J Phys Chem B. 2013 Apr 25;117(16):4406-11
references: 23256598
references: Anal Chem. 2009 Apr 15;81(8):2832-9
references: 19298056
references: Chem Soc Rev. 2010 Mar;39(3):1133-52
references: 20179829
references: FEBS Lett. 1999 Jun 11;452(3):267-71
references: 10386604
references: Nucleus. 2012 Sep-Oct;3(5):404-10
references: 22825571
references: Rep Prog Phys. 2012 Oct;75(10):106601
references: 22975868
references: J Cell Biol. 1984 Jul;99(1 Pt 1):42-52
references: 6736132
references: Nature. 2000 Aug 10;406(6796):593-9
references: 10949293
references: J Biol Chem. 2002 Dec 20;277(51):49562-8
references: 12393868
references: Prog Neurobiol. 2010 Apr;90(4):498-510
references: 20097254
references: Nucleic Acids Res. 2008 Feb;36(3):e15
references: 18202078
references: Nat Genet. 2003 Mar;33 Suppl:245-54
references: 12610534
references: Nature. 2011 Mar 3;471(7336):68-73
references: 21289626
references: Proc Natl Acad Sci U S A. 2012 Jun 5;109(23):9143-8
references: 22615374
references: Science. 2004 Nov 26;306(5701):1571-3
references: 15567867
references: Stem Cells. 2007 Apr;25(4):1037-46
references: 17272500
references: Biophys J. 2009 Jul 22;97(2):544-53
references: 19619469
references: Anal Bioanal Chem. 2004 Apr;378(7):1678-92
references: 15007591
references: Annu Rev Pharmacol Toxicol. 2008;48:257-76
references: 17883328
references: Nano Lett. 2012 Jul 11;12(7):3861-6
references: 22698062
references: Expert Rev Med Devices. 2013 Jan;10(1):1-6
references: 23278216
references: Cell. 2007 Feb 23;128(4):669-81
references: 17320505
references: Nucleic Acids Res. 2006 Jan 05;34(1):e2
references: 16397291
references: Biochem Biophys Res Commun. 1973 Apr 16;51(4):956-66
references: 4349999
references: Nat Biotechnol. 2007 Feb;25(2):207-15
references: 17287758
references: Biophys J. 2002 Dec;83(6):3619-25
references: 12496129
references: ACS Nano. 2012 Sep 25;6(9):7928-34
references: 22816516
references: Nature. 2005 Jul 7;436(7047):138-41
references: 16001076
references: Proc Natl Acad Sci U S A. 1975 Jan;72(1):205-9
references: 164015
references: Anal Chem. 2010 Mar 15;82(6):2480-7
references: 20184350
references: Proc Natl Acad Sci U S A. 1973 May;70(5):1316-20
references: 4351171
references: Phys Rev E Stat Nonlin Soft Matter Phys. 2006 Apr;73(4 Pt 1):041927
references: 16711856
references: J Am Acad Child Adolesc Psychiatry. 2010 Aug;49(8):794-809
references: 20643313
references: Nano Lett. 2008 Jun;8(6):1683-8
references: 18459741
references: Lab Chip. 2012 Jun 21;12(12):2190-8
references: 22566096
references: Science. 2005 Jun 3;308(5727):1466-9
references: 15933200
references: Nucleic Acids Res. 2012 Oct;40(18):8803-17
references: 22790986
references: Lab Chip. 2011 Sep 7;11(17):2842-8
references: 21750827
references: Proc Natl Acad Sci U S A. 2013 May 7;110(19):7772-7
references: 23610441
references: Nat Protoc. 2008;3(6):1032-45
references: 18536650