title: Molecular characterization of cold-responsive basic helix-loop-helix transcription factors MabHLHs that interact with MaICE1 in banana fruit.
abstract: Basic helix-loop-helix (bHLH) transcription factors (TFs) are ubiquitously involved in the response of higher plants to various abiotic stresses. However, little is known about bHLH TFs involved in the cold stress response in economically important fruits. Here, five novel full-length bHLH genes, designated as MabHLH1-MabHLH5, were isolated and characterized from banana fruit. Gene expression profiles revealed that MabHLH1/2/4 were induced by cold stress and methyl jasmonate (MeJA) treatment. Transient assays in tobacco BY2 protoplasts showed that MabHLH1/2/4 promoters were activated by cold stress and MeJA treatments. Moreover, protein-protein interaction analysis demonstrated that MabHLH1/2/4 not only physically interacted with each other to form hetero-dimers in the nucleus, but also interacted with an important upstream component of cold signaling MaICE1, with different interaction domains at their N-terminus. These results indicate that banana fruit cold-responsive MabHLHs may form a big protein complex in the nucleus with MaICE1. Taken together, our findings advance our understanding of the possible involvement of bHLH TFs in the regulatory network of ICE-CBF cold signaling pathway.
authors: State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources/Guangdong Key Laboratory for Postharvest Science, College of Horticultural Science, South China Agricultural University, Guangzhou, 510642, People's Republic of China.
authors: Huan-Huan
authors: HH
authors: Peng
authors: Wei
authors: W
authors: Shan
authors: Jian-Fei
authors: JF
authors: Kuang
authors: Wang-Jin
authors: WJ
authors: Lu
authors: Jian-Ye
authors: JY
authors: Chen
mesh_terms: D000085:Acetates / Q000494:pharmacology; D000222:Adaptation, Physiological / Q000187:drug effects / Q000235:genetics; D000595:Amino Acid Sequence; D051792:Basic Helix-Loop-Helix Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D003080:Cold Temperature; D003517:Cyclopentanes / Q000494:pharmacology; D005453:Fluorescence; D005638:Fruit / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D017343:Genes, Plant / Q000235:genetics; D008969:Molecular Sequence Data; D028521:Musa / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D054883:Oxylipins / Q000494:pharmacology; D010802:Phylogeny; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic; D011485:Protein Binding / Q000187:drug effects; D054730:Protein Interaction Domains and Motifs; D055503:Protein Multimerization / Q000187:drug effects; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D013347:Subcellular Fractions / Q000187:drug effects / Q000378:metabolism; D014026:Nicotiana / Q000187:drug effects / Q000235:genetics
chemical_list: D000085:Acetates; D051792:Basic Helix-Loop-Helix Proteins; D003517:Cyclopentanes; D054883:Oxylipins; D010940:Plant Proteins; C072239:methyl jasmonate
references: Proc Natl Acad Sci U S A. 1997 Feb 4;94(3):1035-40
references: 9023378
references: Mol Plant. 2011 Mar;4(2):279-88
references: 21242320
references: Theor Appl Genet. 2003 Nov;107(8):1402-9
references: 12920519
references: Curr Opin Plant Biol. 2007 Oct;10(5):495-502
references: 17720613
references: Planta. 2011 Aug;234(2):377-90
references: 21505864
references: Plant J. 2011 Jan;65(2):319-28
references: 21223395
references: Plant Cell. 2008 Feb;20(2):337-52
references: 18252845
references: Anal Biochem. 1994 Nov 15;223(1):7-12
references: 7535022
references: Mol Plant. 2012 Jan;5(1):73-84
references: 21746701
references: Plant Cell Environ. 2012 Nov;35(11):1884-97
references: 22519753
references: Crit Rev Biotechnol. 2011 Jun;31(2):186-92
references: 20919819
references: Genes Dev. 2000 Sep 15;14(18):2377-91
references: 10995393
references: Trends Plant Sci. 2007 Oct;12(10):444-51
references: 17855156
references: Gene. 2009 Jan 15;429(1-2):98-103
references: 19026725
references: J Mol Evol. 2003 Jun;56(6):742-50
references: 12911037
references: J Biol Chem. 2006 Sep 29;281(39):28964-74
references: 16867983
references: Plant Cell. 2011 Sep;23(9):3089-100
references: 21963667
references: Plant Cell. 2011 Feb;23(2):701-15
references: 21335373
references: EMBO J. 2007 Nov 14;26(22):4756-67
references: 17948056
references: J Exp Bot. 2010 Jul;61(12):3305-20
references: 20566565
references: Genes Dev. 2003 Apr 15;17(8):1043-54
references: 12672693
references: Plant Cell Environ. 2013 Jan;36(1):30-51
references: 22651394
references: Curr Biol. 2005 Nov 22;15(22):1998-2006
references: 16303558
references: Plant J. 1994 Mar;5(3):421-7
references: 8180625
references: Plant J. 2011 Mar;65(6):907-21
references: 21332845
references: Plant Cell. 2008 Jul;20(7):1775-85
references: 18641265
references: Plant J. 2011 Apr;66(1):94-116
references: 21443626
references: Methods Mol Biol. 2010;639:39-55
references: 20387039
references: Plant Physiol. 2006 Aug;141(4):1167-84
references: 16896230
references: Physiol Plant. 2008 Mar;132(3):318-28
references: 18275463
references: New Phytol. 2012 Sep;195(4):737-751
references: 22816520
references: BMC Plant Biol. 2012 Feb 15;12:22
references: 22336381
references: Planta. 2012 Jun;235(6):1091-105
references: 22526498
references: Proc Natl Acad Sci U S A. 2006 May 23;103(21):8281-6
references: 16702557
references: Mol Biol Evol. 2003 May;20(5):735-47
references: 12679534
references: Plant J. 2010 Mar;61(6):1041-52
references: 20409277
references: Plant Cell. 2011 May;23(5):1795-814
references: 21551388
references: Plant Cell. 2005 Nov;17(11):3155-75
references: 16214899
references: Plant Cell. 1998 Aug;10(8):1391-406
references: 9707537
references: Plant Signal Behav. 2010 Aug;5(8):948-52
references: 20699657
references: J Biol Chem. 2006 Dec 8;281(49):37636-45
references: 17015446
references: Plant Cell. 2007 Apr;19(4):1403-14
references: 17416732
references: J Exp Bot. 2012 Jan;63(1):293-304
references: 21940717
references: Biosci Biotechnol Biochem. 2005 May;69(5):1042-4
references: 15914931
references: Plant Physiol. 2010 Jul;153(3):1398-412
references: 20472752
references: Genome Res. 2001 May;11(5):754-70
references: 11337472
references: Plant Sci. 2011 Jan;180(1):3-11
references: 21421341
references: Proc Natl Acad Sci U S A. 2004 Jun 29;101(26):9873-8
references: 15205481
references: Plant Cell. 2003 Aug;15(8):1749-70
references: 12897250
references: Biochim Biophys Acta. 2012 Feb;1819(2):186-94
references: 21867784
references: Genes Dev. 2001 Apr 1;15(7):912-24
references: 11297514