title: Complementation of the Fo c subunit of Escherichia coli with that of Streptococcus mutans and properties of the hybrid FoF1 ATP synthase.
abstract: The c subunit of Streptococcus mutans ATP synthase (FoF1) is functionally exchangeable with that of Escherichia coli, since E. coli with a hybrid FoF1 is able to grow on minimum succinate medium through oxidative phosphorylation. E. coli F1 bound to the hybrid Fo with the S. mutans c subunit showed N,N'-dicyclohexylcarbodiimide-sensitive ATPase activity similar to that of E. coli FoF1. Thus, the S. mutans c subunit assembled into a functional Fo together with the E. coli a and b subunits, forming a normal F1 binding site. Although the H(+) pathway should be functional, as was suggested by the growth on minimum succinate medium, ATP-driven H(+) transport could not be detected with inverted membrane vesicles in vitro. This observation is partly explained by the presence of an acidic residue (Glu-20) in the first transmembrane helix of the S. mutans c subunit, since the site-directed mutant carrying Gln-20 partly recovered the ATP-driven H(+) transport. Since S. mutans is recognized to be a primary etiological agent of human dental caries and is one cause of bacterial endocarditis, our system that expresses hybrid Fo with the S. mutans c subunit would be helpful to find antibiotics and chemicals specifically directed to S. mutans.
authors: Department of Molecular Biology, School of Pharmacy, Iwate Medical University, Nishitokuta, Yahaba, Shiwa-gun, Iwate, Japan.
authors: Makoto
authors: M
authors: Araki
authors: Kazuya
authors: K
authors: Hoshi
authors: Masasuke
authors: M
authors: Fujiwara
authors: Yuka
authors: Y
authors: Sasaki
authors: Hideo
authors: H
authors: Yonezawa
authors: Hidenobu
authors: H
authors: Senpuku
authors: Atsuko
authors: A
authors: Iwamoto-Kihara
authors: Masatomo
authors: M
authors: Maeda
mesh_terms: D000595:Amino Acid Sequence; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology; D005816:Genetic Complementation Test; D005947:Glucose / Q000378:metabolism; D021122:Protein Subunits; D006180:Proton-Translocating ATPases / Q000235:genetics / Q000378:metabolism*; D013295:Streptococcus mutans / Q000235:genetics / Q000378:metabolism*
chemical_list: D021122:Protein Subunits; D006180:Proton-Translocating ATPases; D005947:Glucose
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