title: Poly(N-isopropylacrylamide)-stabilized gold nanoparticles in combination with tricationic branched phenylene-ethynylene fluorophore for protein identification.
abstract: Gold nanoparticles stabilized by thermoresponsive polymer, poly(N-isopropylacrylamide) (PNIPAM-AuNPs) were prepared by surface grafting of thiol-terminated PNIPAM onto citrate-stabilized AuNPs. The color change of the PNIPAM-AuNPs solution from red to blue-purple without precipitation when the solution was heated to 40 °C, above the lower critical solution temperature (LCST) of PNIPAM, indicated the thermoresponsive property of the synthesized AuNPs. PNIPAM-AuNPs were used to detect proteins by chemical nose approach based on fluorescence quenching of fluorophore by AuNPs. An array-based sensing platform for detection of six proteins, namely bovine serum albumin, lysozyme, fibrinogen, concanavalin A, hemoglobin, holo-transferrin human can be successfully developed from the PNIPAM-AuNPs having different molecular weights (4 and 8 kDa) and conformation (varied heat treatment from 25 to 40 °C) in combination with a tricationic branched phenylene-ethynylene fluorophore. From principal component analysis (PCA) followed by linear discriminant analysis (LDA), 100% accuracy of protein classification using a leave-one-out (LOO) approach can be achieved by using only two types of PNIPAM-AuNPs.
authors: Program in Petrochemistry and Polymer Science, Faculty of Science, Chulalongkorn University , Phayathai Road, Pathumwan, Bangkok 10330, Thailand.
authors: Keerati
authors: K
authors: Kusolkamabot
authors: Pornpen
authors: P
authors: Sae-ung
authors: Nakorn
authors: N
authors: Niamnont
authors: Kanet
authors: K
authors: Wongravee
authors: Mongkol
authors: M
authors: Sukwattanasinitt
authors: Voravee P
authors: VP
authors: Hoven
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry*; D000480:Alkynes / Q000737:chemistry*; D000818:Animals; D002412:Cations / Q000737:chemistry; D002417:Cattle; D003208:Concanavalin A / Q000032:analysis; D005340:Fibrinogen / Q000032:analysis; D006046:Gold / Q000737:chemistry*; D006454:Hemoglobins / Q000032:analysis; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry*; D015394:Molecular Structure; D009113:Muramidase / Q000032:analysis / Q000378:metabolism; D010316:Particle Size; D012710:Serum Albumin, Bovine / Q000032:analysis; D013499:Surface Properties; D013696:Temperature; D014168:Transferrin / Q000032:analysis
chemical_list: D000180:Acrylic Resins; D000480:Alkynes; D002412:Cations; D006454:Hemoglobins; D014168:Transferrin; D003208:Concanavalin A; C052970:poly-N-isopropylacrylamide; D012710:Serum Albumin, Bovine; D006046:Gold; D005340:Fibrinogen; D009113:Muramidase