title: γ-Cyclodextrin-polyurethane copolymer adsorbent for selective removal of endotoxin from DNA solution.
abstract: Copolymer particles for removal of endotoxins (lipopolysaccharides, LPSs) were prepared by suspension copolymerization of γ-cyclodextrin (CyD) and 1,6-hexamethylenediisocyanate. The LPS-removing activity of the copolymer particles was compared with that of poly(ε-lysine)-immobilized Cellufine (cationic adsorbent) or polystyrene particles (hydrophobic adsorbent) by a batch method. When DNA was present in solution with LPSs under physiological conditions (pH 6.0, ionic strength of μ = 0.05-0.8), LPS-removing activity of the cationic or hydrophobic adsorbent was unsatisfactory because both the DNA and the LPSs were adsorbed onto each adsorbent. By contrast, the copolymer particles with γ-CyD cavity (CyD content: 14-20 mol%) could selectively remove LPSs from a DNA solution (50 μg ml(-1), pH 6.0, and μ = 0.05-0.2) containing LPSs (15 EU ml(-1)) without the adsorption of DNA. The residual concentration of LPSs in the treated DNA solution was below 0.1 EU ml(-1), and the recovery of DNA was 99%.
authors: Department of Applied Chemistry and Biochemistry, Graduate School of Science and Technology, Kumamoto University, Chuo-ku, Kumamoto 860-8555, Japan; Japan Science and Technology (JST), Core Research for Evolutional Science and Technology (CREST), Chiyoda-ku, Tokyo 102-0075, Japan. Electronic address: msakata@gpo.kumamoto-u.ac.jp.
authors: Masayo
authors: M
authors: Sakata
authors: Koji
authors: K
authors: Uezono
authors: Kasane
authors: K
authors: Kimura
authors: Masami
authors: M
authors: Todokoro
mesh_terms: D000327:Adsorption; D000818:Animals; D003485:Cyanates / Q000737:chemistry; D004247:DNA / Q000302:isolation & purification*; D004926:Escherichia coli / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D017953:Isocyanates; D008070:Lipopolysaccharides / Q000302:isolation & purification*; D008297:Male; D008863:Microspheres; D009994:Osmolar Concentration; D058105:Polymerization; D011140:Polyurethanes / Q000737:chemistry*; D012474:Salmon; D012680:Sensitivity and Specificity; D052616:Solid Phase Extraction; D012996:Solutions; D017550:Spectroscopy, Fourier Transform Infrared; D013094:Spermatozoa / Q000737:chemistry; D047408:gamma-Cyclodextrins / Q000737:chemistry*
chemical_list: D003485:Cyanates; D017953:Isocyanates; D008070:Lipopolysaccharides; D011140:Polyurethanes; D012996:Solutions; D047408:gamma-Cyclodextrins; C015262:1,6-hexamethylene diisocyanate; D004247:DNA; C023792:gamma-cyclodextrin
keywords: Cavity; Cyclodextrin; DNA; Endotoxin removal; Lipopolysaccharides