title: Therapeutic effect of Artemia enriched with Escherichia coli expressing double-stranded RNA in the black tiger shrimp Penaeus monodon.
abstract: We exploited Artemia as a double-stranded (ds)RNA-delivery system to combat viral diseases in shrimp. First, the transformed Escherichia coli (E. coli) expressing red fluorescent protein (RFP) was tested in the Artemia enrichment process. RFP signals detectable in the gut of Artemia under confocal microscope were evident for the successful encapsulation. Second, the Artemia enrichment process was performed using E. coli producing Laem-Singh virus (LSNV)-specific dsRNA, which has been previously shown to inhibit the viral infection in the black tiger shrimp Penaeus monodon by intramuscular injection and oral administration. The enriched Artemia nauplii were confirmed to contain dsRNA-LSNV by RT-PCR, and were subjected to the feeding test with P. monodon postlarvae. Quantitative RT-PCR indicated that a number of LSNV copies in most of the treated shrimp were, at least, 1000-fold lower than the untreated controls. During 11-17weeks after feeding, average body weight of the treated group was markedly increased relative to the control group. A smaller differential growth rate of the treated group as compared to the control was also noticed. These results suggested that feeding shrimp with the dsRNA-enriched Artemia can eliminate LSNV infection, which is the cause of retarded growth in P. monodon. The present study reveals for the first time the therapeutic effect of dsRNA-enriched Artemia for shrimp disease control.
authors: Center of Excellence for Shrimp Molecular Biology and Biotechnology (Centex Shrimp), Bangkok, Thailand; Department of Biotechnology, Faculty of Science, Mahidol University, Bangkok 10400, Thailand.
authors: Thitiporn
authors: T
authors: Thammasorn
authors: Parinyachat
authors: P
authors: Somchai
authors: Chaowanee
authors: C
authors: Laosutthipong
authors: Sarocha
authors: S
authors: Jitrakorn
authors: Somjai
authors: S
authors: Wongtripop
authors: Siripong
authors: S
authors: Thitamadee
authors: Boonsirm
authors: B
authors: Withyachumnarnkul
authors: Vanvimon
authors: V
authors: Saksmerprome
mesh_terms: D000818:Animals; D001156:Artemia / Q000382:microbiology / Q000821:virology*; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D018014:Gene Transfer Techniques; D005822:Genetic Vectors / Q000235:genetics / Q000378:metabolism; D033561:Penaeidae / Q000382:microbiology / Q000821:virology*; D012328:RNA Viruses / Q000235:genetics / Q000378:metabolism; D012367:RNA, Viral / Q000235:genetics* / Q000378:metabolism
chemical_list: D012367:RNA, Viral
keywords: Artemia; Bioencapsulation; Laem-Singh virus; Penaeus monodon shrimp; dsRNA