title: Combined effect of dimethyl dicarbonate (DMDC) and nisin on indigenous microorganisms of litchi juice and its microbial shelf life.
abstract: The individual and combined influences of dimethyl dicarbonate (DMDC) and nisin (200 IU/mL) at mild heat on the inactivation of indigenous microorganisms in litchi juice, including bacteria, molds and yeasts (M&Y), were investigated. The fresh litchi juice with or without nisin were exposed to 250 mg/L DMDC at 30, 40, or 45 °C for 0.5, 1, 2, 3, 4, or 6 h. A complete inactivation of M&Y in the litchi juice with or without nisin was achieved as exposed to 250 mg/L DMDC at 30, 40, or 45 °C for 0.5 h. The bacteria, especially Bacillus sp. and Leuconstoc mesenteroides showed higher resistance than M&Y in the litchi juice. Bacillus sp. and Leuconstoc mesenteroides in the litchi juice was not completely inactivated by 250 mg/L DMDC at 30, 40, or 45 °C. However, nisin addition can enhanced the inactivation of these bacteria by DMDC, and nisin and DMDC also showed a synergistic effect on the inactivation of bacteria. M&Y and bacteria were not detected in the litchi juice added with 200 IU/mL nisin as exposed to 250 mg/L DMDC at 45 °C for 3 h. In addition, microbial shelf life of the litchi juice during storage at 4 °C also was evaluated as treated by 250 mg/L DMDC or combination with nisin at 45 °C for 3 h.
authors: Sericulture and Agri-food Research Insti., Guangdong Academy of Agricultural Sciences, Guangzhou 510610, China.
authors: Yuanshan
authors: Y
authors: Yu
authors: Jijun
authors: J
authors: Wu
authors: Gengsheng
authors: G
authors: Xiao
authors: Yujuan
authors: Y
authors: Xu
authors: Daobang
authors: D
authors: Tang
authors: Yulong
authors: Y
authors: Chen
authors: Yousheng
authors: Y
authors: Zhang
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001407:Bacillus / Q000187:drug effects / Q000254:growth & development / Q000302:isolation & purification; D001628:Beverages / Q000382:microbiology*; D015169:Colony Count, Microbial; D004047:Diethyl Pyrocarbonate / Q000031:analogs & derivatives* / Q000494:pharmacology; D004357:Drug Synergism; D005506:Food Contamination / Q000517:prevention & control; D005511:Food Handling; D005516:Food Microbiology; D005519:Food Preservation; D005638:Fruit; D007934:Leuconostoc / Q000187:drug effects / Q000254:growth & development / Q000302:isolation & purification; D032125:Litchi / Q000382:microbiology*; D009561:Nisin / Q000494:pharmacology*; D014944:World Health Organization
chemical_list: D000900:Anti-Bacterial Agents; D009561:Nisin; C061815:dimethyl pyrocarbonate; D004047:Diethyl Pyrocarbonate
keywords: dimethyl dicarbonate (DMDC); inactivation; litchi juice; microorganisms; nisin; shelf life