title: The effect of bacterial challenge on ferritin regulation in the yellow fever mosquito, Aedes aegypti.
abstract: Secreted ferritin is the major iron storage and transport protein in insects. Here, we characterize the message and protein expression profiles of yellow fever mosquito (Aedes aegypti) ferritin heavy chain homologue (HCH) and light chain homologue (LCH) subunits in response to iron and bacterial challenge. In vivo experiments demonstrated tissue-specific regulation of HCH and LCH expression over time post-blood meal (PBM). Transcriptional regulation of HCH and LCH was treatment specific, with differences in regulation for naïve versus mosquitoes challenged with heat-killed bacteria (HKB). Translational regulation by iron regulatory protein (IRP) binding activity for the iron-responsive element (IRE) was tissue-specific and time-dependent PBM. However, mosquitoes challenged with HKB showed little change in IRP/IRE binding activity compared to naïve animals. The changes in ferritin regulation and expression in vivo were confirmed with in vitro studies. We challenged mosquitoes with HKB followed by a blood meal to determine the effects on ferritin expression, and demonstrate a synergistic, time-dependent regulation of expression for HCH and LCH.
authors: Department of Nutritional Sciences, College of Agriculture and Life Sciences, the University of Arizona, Tucson, Arizona, 85721, USA.
authors: Dawn L
authors: DL
authors: Geiser
authors: Guoli
authors: G
authors: Zhou
authors: Jonathan J
authors: JJ
authors: Mayo
authors: Joy J
authors: JJ
authors: Winzerling
mesh_terms: D000330:Aedes / Q000235:genetics / Q000378:metabolism* / Q000382:microbiology*; D000818:Animals; D001412:Bacillus subtilis / Q000502:physiology*; D002460:Cell Line; D002470:Cell Survival; D004926:Escherichia coli / Q000502:physiology*; D005216:Fat Body / Q000166:cytology / Q000378:metabolism; D005260:Female; D005293:Ferritins / Q000378:metabolism*; D005296:Ferrous Compounds; D041981:Gastrointestinal Tract / Q000166:cytology / Q000378:metabolism; D005786:Gene Expression Regulation / Q000502:physiology; D006358:Hot Temperature; D007501:Iron; D035925:Iron-Regulatory Proteins / Q000502:physiology; D007814:Larva / Q000166:cytology / Q000382:microbiology; D008301:Maleimides; D000075163:Metallocenes; D010053:Ovary / Q000166:cytology / Q000378:metabolism; D011485:Protein Binding; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D005296:Ferrous Compounds; D035925:Iron-Regulatory Proteins; D008301:Maleimides; D000075163:Metallocenes; C100759:N-(2-ferrocene-ethyl)maleimide; D005293:Ferritins; D007501:Iron
keywords: Aedes aegypti; IRP; bacteria; ferritin; iron; mosquito
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