title: Identification of Borrelia burgdorferi ospC genotypes in canine tissue following tick infestation: implications for Lyme disease vaccine and diagnostic assay design.
abstract: In endemic regions, Lyme disease is a potential health threat to dogs. Canine Lyme disease manifests with arthritis-induced lameness, anorexia, fever, lethargy, lymphadenopathy and, in some cases, fatal glomerulonephritis. A recent study revealed that the regional mean for the percentage of seropositive dogs in the north-east of the USA is 11.6%. The outer surface protein C (OspC) of Lyme disease spirochetes is an important virulence factor required for the establishment of infection in mammals. It is a leading candidate in human and canine Lyme disease vaccine development efforts. Over 30 distinct ospC phyletic types have been defined. It has been hypothesized that ospC genotype may influence mammalian host range. In this study, Ixodes scapularis ticks collected from the field in Rhode Island were assessed for infection with B. burgdorferi. Ticks were fed on purpose bred beagles to repletion and infection of the dogs was assessed through serology and PCR. Tissue biopsies (n=2) were collected from each dog 49 days post-tick infestation (dpi) and the ospC genotype of the infecting strains determined by direct PCR of DNA extracted from tissue or by PCR after cultivation of spirochetes from biopsy samples. The dominant ospC types associated with B. burgdorferi canine infections differed from those associated with human infection, indicating a relationship between ospC sequence and preferred host range. Knowledge of the most common ospC genotypes associated specifically with infection of dogs will facilitate the rational design of OspC-based canine Lyme disease vaccines and diagnostic assays.
authors: Department of Microbiology and Immunology, Virginia Commonwealth University, Richmond, VA 23298-0678, USA.
authors: D V L
authors: DV
authors: Rhodes
authors: C G
authors: CG
authors: Earnhart
authors: T N
authors: TN
authors: Mather
authors: P F M
authors: PF
authors: Meeus
authors: R T
authors: RT
authors: Marconi
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood / Q000235:genetics / Q000378:metabolism; D000942:Antigens, Bacterial / Q000235:genetics* / Q000378:metabolism; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D001428:Bacterial Vaccines / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D025065:Borrelia burgdorferi / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D004283:Dog Diseases / Q000382:microbiology* / Q000469:parasitology*; D004285:Dogs; D005838:Genotype; D018884:Ixodes / Q000382:microbiology / Q000502:physiology; D008193:Lyme Disease / Q000382:microbiology / Q000662:veterinary*; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012237:Rhode Island; D017422:Sequence Analysis, DNA / Q000662:veterinary; D013984:Tick Infestations / Q000469:parasitology / Q000662:veterinary; D037521:Virulence Factors / Q000097:blood / Q000235:genetics / Q000378:metabolism
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001428:Bacterial Vaccines; C079616:OspC protein; D037521:Virulence Factors
keywords: Borrelia burgdorferi; Canine; Lyme disease; OspC; Ticks; Vaccine
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