title: The first transformation method for the thermo-acidophilic archaeon Thermoplasma acidophilum.
abstract: A transformation method yielding up to 10(4) transformants per μg circular DNA was developed for Thermoplasma acidophilum. The method is based on a natural DNA uptake process in which T. acidophilum cells keep their integrity and turn competent at pH 3.5 and 58°C. Shuttle vector maintenance could not be detected, since the used Nov(R) gyraseB gene integrated into its chromosomal counterpart by homologous recombination.
authors: Department of Environmental Protection & Environmental Safety, Szent István University, Gödöllő 2100, Hungary; Department of Microbiology, Central Environmental and Food Science Research Institute, Budapest 1022, Hungary.
authors: Erzsébet
authors: E
authors: Baka
authors: Sándor
authors: S
authors: Varga
authors: Cédric
authors: C
authors: Hobel
authors: Roland Wilhelm
authors: RW
authors: Knispel
authors: Csaba
authors: C
authors: Fekete
authors: Milán
authors: M
authors: Ivanics
authors: Balázs
authors: B
authors: Kriszt
authors: István
authors: I
authors: Nagy
authors: József
authors: J
authors: Kukolya
mesh_terms: D019847:Chromosomes, Archaeal / Q000235:genetics; D003001:Cloning, Molecular; D003470:Culture Media; D019641:DNA, Archaeal / Q000235:genetics; D004270:DNA, Circular / Q000235:genetics; D004352:Drug Resistance, Microbial; D018014:Gene Transfer Techniques; D005822:Genetic Vectors / Q000235:genetics; D006863:Hydrogen-Ion Concentration; D009675:Novobiocin / Q000494:pharmacology; D011401:Promoter Regions, Genetic; D017422:Sequence Analysis, DNA; D013696:Temperature; D013822:Thermoplasma / Q000235:genetics*; D014170:Transformation, Genetic
chemical_list: D003470:Culture Media; D019641:DNA, Archaeal; D004270:DNA, Circular; D009675:Novobiocin
keywords: Archaea; Homologous recombination; Novobiocin; Plasmid; Thermoplasma acidophilum; Transformation