title: Generation of ligand specificity and modes of oligomerization in β-prism I fold lectins.
abstract: β-Prism I fold lectins constitute one of the five widely occurring structural classes of plant lectins. Each single domain subunit is made up of three Greek key motifs arranged in a threefold symmetric fashion. The threefold symmetry is not reflected in the sequence except in the case of the lectin from banana, a monocot, which carries two sugar-binding sites instead of the one in other lectins of known three-dimensional structure, all from dicots. This is believed to be a consequence of the different evolutionary paths followed by the lectin in monocots and dicots. The galactose-specific lectins among them have two chains produced by posttranslational proteolysis and contain three aromatic residues at the binding site. The extended binding sites of galactose- and mannose-specific lectins have been thoroughly characterized. Ligand binding at the sites involves both conformational selection and induced fit. Molecular plasticity of some of the lectins in the family has been characterized. The plasticity appears to be such as to promote variability in quaternary association which could be dimeric, tetrameric, or octameric. Structural and evolutionary reasons for the variability have been explored, and the relation of oligomerization to ligand binding and conformational selection investigated.
authors: Molecular Biophysics Unit, Indian Institute of Science, Bangalore, India. Electronic address: mv@mbu.iisc.ernet.in.
authors: Thyageshwar
authors: T
authors: Chandran
authors: Alok
authors: A
authors: Sharma
authors: Mamannamana
authors: M
authors: Vijayan
mesh_terms: D000595:Amino Acid Sequence; D001665:Binding Sites; D018360:Crystallography, X-Ray; D037102:Lectins; D008958:Models, Molecular; D008969:Molecular Sequence Data; D037121:Plant Lectins
chemical_list: D037102:Lectins; D037121:Plant Lectins
keywords: Conformational selection; Evolution; Induced fit; Lectin–sugar interactions; Plant lectins; Quaternary structure; Sugar specificity