title: Molecular epidemiology of early and acute HIV type 1 infections in the United States Navy and Marine Corps, 2005-2010.
abstract: The U.S. military represents a unique population within the human immunodeficiency virus 1 (HIV-1) pandemic. The last comprehensive study of HIV-1 in members of the U.S. Navy and Marine Corps (Sea Services) was completed in 2000, before large-scale combat operations were taking place. Here, we present molecular characterization of HIV-1 from 40 Sea Services personnel who were identified during their seroconversion window and initially classified as HIV-1 negative during screening. Protease/reverse transcriptase (pro/rt) and envelope (env) sequences were obtained from each member of the cohort. Phylogenetic analyses were carried out on these regions to determine relatedness within the cohort and calculate the most recent common ancestor for the related sequences. We identified 39 individuals infected with subtype B and one infected with CRF01_AE. Comparison of the pairwise genetic distance of Sea Service sequences and reference sequences in the env and pro/rt regions showed that five samples were part of molecular clusters, a group of two and a group of three, confirmed by single genome amplification. Real-time molecular monitoring of new HIV-1 acquisitions in the Sea Services may have a role in facilitating public health interventions at sites where related HIV-1 infections are identified.
authors: 1 US Military HIV Research Program, Walter Reed Army Institute of Research , Silver Spring, Maryland.
authors: Richard A
authors: RA
authors: Heipertz
authors: Eric
authors: E
authors: Sanders-Buell
authors: Gustavo
authors: G
authors: Kijak
authors: Shana
authors: S
authors: Howell
authors: Michelle
authors: M
authors: Lazzaro
authors: Linda L
authors: LL
authors: Jagodzinski
authors: John
authors: J
authors: Eggleston
authors: Sheila
authors: S
authors: Peel
authors: Jennifer
authors: J
authors: Malia
authors: Adam
authors: A
authors: Armstrong
authors: Nelson L
authors: NL
authors: Michael
authors: Jerome H
authors: JH
authors: Kim
authors: Robert J
authors: RJ
authors: O'Connell
authors: Paul T
authors: PT
authors: Scott
authors: David M
authors: DM
authors: Brett-Major
authors: Sodsai
authors: S
authors: Tovanabutra
mesh_terms: D016000:Cluster Analysis; D015658:HIV Infections / Q000453:epidemiology* / Q000821:virology*; D015497:HIV-1 / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D008889:Military Personnel; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics; D017422:Sequence Analysis, DNA; D014481:United States / Q000453:epidemiology; D014764:Viral Proteins / Q000235:genetics
chemical_list: D012367:RNA, Viral; D014764:Viral Proteins