title: Matrix suppression as a guideline for reliable quantification of peptides by matrix-assisted laser desorption ionization.
abstract: We propose to divide matrix suppression in matrix-assisted laser desorption ionization into two parts, normal and anomalous. In quantification of peptides, the normal effect can be accounted for by constructing the calibration curve in the form of peptide-to-matrix ion abundance ratio versus concentration. The anomalous effect forbids reliable quantification and is noticeable when matrix suppression is larger than 70%. With this 70% rule, matrix suppression becomes a guideline for reliable quantification, rather than a nuisance. A peptide in a complex mixture can be quantified even in the presence of large amounts of contaminants, as long as matrix suppression is below 70%. The theoretical basis for the quantification method using a peptide as an internal standard is presented together with its weaknesses. A systematic method to improve quantification of high concentration analytes has also been developed.
authors: Department of Chemistry, Seoul National University , Seoul 151-742, Korea.
authors: Sung Hee
authors: SH
authors: Ahn
authors: Yong Jin
authors: YJ
authors: Bae
authors: Jeong Hee
authors: JH
authors: Moon
authors: Myung Soo
authors: MS
authors: Kim
mesh_terms: D002138:Calibration / Q000592:standards; D010455:Peptides / Q000032:analysis*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods* / Q000592:standards*
chemical_list: D010455:Peptides