title: A novel production process for optically pure L-lactic acid from kitchen refuse using a bacterial consortium at high temperatures.
abstract: We investigated L-lactic acid production in static batch fermentation of kitchen refuse using a bacterial consortium from marine-animal-resource (MAR) composts at temperatures ranging from 30 to 65 °C. At relatively low temperatures butyric acid accumulated, whereas at higher temperatures L-lactic acid was produced. In particular, fermentation at 50 °C produced 34.5 g L(-1) L-lactic acid with 90% lactic acid selectivity and 100% optical purity. Denaturing gradient gel electrophoresis indicated that dominant bacteria present in the original MAR composts diminished rapidly and Bacillus coagulans strains became the dominant contributors to L-lactic acid production at 45, 50 and 55 °C. This is the first report of the achievement of 100% optical purity of L-lactic acid using a bacterial consortium.
authors: Laboratory of Soil Microbiology, Division of Applied Molecular Microbiology and Biomass Chemistry, Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan; Institute of Advanced Study, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan.
authors: Yukihiro
authors: Y
authors: Tashiro
authors: Laboratory of Soil Microbiology, Division of Applied Molecular Microbiology and Biomass Chemistry, Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan.
authors: Hiroko
authors: H
authors: Matsumoto
authors: Japan Eco-science Co. Ltd., 11-1 Shiomigaokacho, Chuo-ku, Chiba, Chiba 260-0034, Japan.
authors: Hirokuni
authors: H
authors: Miyamoto
authors: Laboratory of Soil Microbiology, Division of Applied Molecular Microbiology and Biomass Chemistry, Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan.
authors: Yuki
authors: Y
authors: Okugawa
authors: Laboratory of Soil Microbiology, Division of Applied Molecular Microbiology and Biomass Chemistry, Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan.
authors: Poudel
authors: P
authors: Pramod
authors: Miroku Co. Ltd., Iwaya 706-27, Kitsuki City, Oita 873-0021, Japan.
authors: Hisashi
authors: H
authors: Miyamoto
authors: Laboratory of Soil Microbiology, Division of Applied Molecular Microbiology and Biomass Chemistry, Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan. Electronic address: kensak@agr.kyushu-u.ac.jp.
authors: Kenji
authors: K
authors: Sakai
mesh_terms: D001407:Bacillus / Q000235:genetics / Q000378:metabolism*; D001673:Biodegradation, Environmental; D020148:Butyric Acid / Q000737:chemistry; D004247:DNA / Q000032:analysis; D058645:Denaturing Gradient Gel Electrophoresis; D005285:Fermentation; D005735:Garbage; D006863:Hydrogen-Ion Concentration; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D019344:Lactic Acid / Q000096:biosynthesis*; D059013:Microbial Consortia; D012336:RNA, Ribosomal, 16S / Q000032:analysis; D012037:Refuse Disposal; D017422:Sequence Analysis, DNA; D013696:Temperature; D013997:Time Factors
chemical_list: D012336:RNA, Ribosomal, 16S; D020148:Butyric Acid; D019344:Lactic Acid; D004247:DNA; D007770:L-Lactate Dehydrogenase
keywords: Bacterial consortium; Kitchen refuse; l-Lactic acid fermentation