title: Influence of nanoparticle-embedded polymeric surfaces on cellular adhesion, proliferation, and differentiation.
abstract: The development of functional substrates to direct cellular organization is important for biomedical applications such as regenerative medicine and biorobotics. In this study, we prepared freestanding polymeric ultrathin films (nanofilms) consisting of poly(lactic acid) (PLA) and magnetic nanoparticles (MNPs), and evaluated the effects of their surface properties on the organization of cardiac-like rat myoblasts (H9c2). We changed surface properties of the PLA nanofilms (i.e., roughness and wettability) as a function of MNPs concentration. We found that the incorporation of MNPs into the nanofilms enhanced both proliferation and adhesion of H9c2 cells. Through the morphological assessment of the differentiated H9c2 cells, we also found that the presence of MNPs significantly increased the fusion index and the surface area of myotubes. In conclusion, the embedding of MNPs is a simple method to tailor the physicochemical properties of the polymeric nanofilms, yet it is an effective approach to enhance the cellular morphogenesis in the field of cardiac tissue engineering for regenerative medicine and biorobotics applications.
authors: Center for Micro-BioRobotics @SSSA, Istituto Italiano di Tecnologia, Viale Rinaldo Piaggio, 34, 56025, Pontedera (PI), Italy; The BioRobotics Institute, Scuola Superiore Sant'Anna, Polo Sant'Anna Valdera, Viale Rinaldo Piaggio, 34, 56025, Pontedera (PI), Italy.
authors: Letizia
authors: L
authors: Ventrelli
authors: Toshinori
authors: T
authors: Fujie
authors: Serena
authors: S
authors: Del Turco
authors: Giuseppina
authors: G
authors: Basta
authors: Barbara
authors: B
authors: Mazzolai
authors: Virgilio
authors: V
authors: Mattoli
mesh_terms: D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects*; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D057927:Hydrophobic and Hydrophilic Interactions; D019344:Lactic Acid / Q000494:pharmacology*; D060328:Magnetic Phenomena; D018625:Microscopy, Atomic Force; D032446:Myoblasts / Q000166:cytology* / Q000187:drug effects; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D011091:Polyesters; D011108:Polymers / Q000494:pharmacology*; D051381:Rats; D013499:Surface Properties
chemical_list: D011091:Polyesters; D011108:Polymers; D019344:Lactic Acid; C033616:poly(lactide)
keywords: cardiac tissue engineering; cellular scaffolds; magnetic nanoparticles; surface roughness; ultrathin films (nanofilms)