title: Molecular identification of a pancreatic lipase-like gene involved in sex pheromone biosynthesis of Bombyx mori.
abstract: Cytoplasmic lipid droplet (LD) lipolysis is regulated by pheromone biosynthesis activating neuropeptide (PBAN) in Bombyx mori. To elucidate the molecular mechanism of cytoplasm LD lipolysis, the pancreatic lipase-like gene in B. mori pheromone glands (PGs), designated as B. mori pancreatic lipase-like gene (BmPLLG), was identified in this study. Spatial expression analysis revealed that BmPLLG is a ubiquitous gene present in all studied tissues, such as PGs, brain, epidermis, egg, midgut, flight muscle and fat body. Temporal expression analysis showed that the BmPLLG transcript begins to express 96 h before eclosion (-96 h), continues to increase, peaks in newly emerged females and steadily decreases after eclosion. Translational expression analysis of BmPLLG using a prepared antiserum demonstrated that BmPLLG was expressed in an age-dependent pattern at different development stages in B. mori. This finding was similar to the transcript expression pattern. Further RNA interference-mediated knockdown of BmPLLG significantly inhibited bombykol production. Overall, these results demonstrated that BmPLLG is involved in PBAN-induced sex pheromone biosynthesis and release.
authors: College of Plant Protection, Henan Agricultural University.
authors: Song-Dou
authors: SD
authors: Zhang
authors: Xun
authors: X
authors: Li
authors: Zhu
authors: Z
authors: Bin
authors: Meng-Fang
authors: MF
authors: Du
authors: Xin-Ming
authors: XM
authors: Yin
authors: Shi-Heng
authors: SH
authors: An
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D012831:Bombyx / Q000235:genetics*; D005233:Fatty Alcohols / Q000378:metabolism; D005260:Female; D008018:Life Cycle Stages; D008049:Lipase / Q000032:analysis / Q000235:genetics*; D008969:Molecular Sequence Data; D009479:Neuropeptides / Q000096:biosynthesis / Q000235:genetics*; D034622:RNA Interference; D012724:Sex Attractants / Q000096:biosynthesis / Q000235:genetics*
chemical_list: D005233:Fatty Alcohols; D009479:Neuropeptides; D012724:Sex Attractants; C006787:bombykol; D008049:Lipase
keywords: Bombyx mori; RNAi; lipase; sex pheromone