title: Human herpesvirus 6 U69 kinase phosphorylates the methylenecyclopropane nucleosides cyclopropavir, MBX 2168, and MBX 1616 to their monophosphates.
abstract: Dihydroxymethyl and monohydroxymethyl methylenecyclopropane nucleosides are effective inhibitors of both variants of human herpesvirus 6 (HHV-6). We investigated involvement of HHV-6 U69 protein kinase in their mechanism of action. Phosphorylation of the dihydroxymethyl analogue cyclopropavir and monohydroxymethyl nucleosides with either a 6-ether moiety (MBX 2168) or a 6-thioether moiety (MBX 1616) with purified U69 was examined. All three compounds were substrates of this viral kinase and had similar Michaelis-Menten kinetic parameters.
authors: Microbiotix, Inc., Worcester, Massachusetts, USA.
authors: Gloria
authors: G
authors: Komazin-Meredith
authors: Steven C
authors: SC
authors: Cardinale
authors: John D
authors: JD
authors: Williams
authors: Norton P
authors: NP
authors: Peet
authors: Mark N
authors: MN
authors: Prichard
authors: Terry L
authors: TL
authors: Bowlin
mesh_terms: D000998:Antiviral Agents / Q000737:chemistry*; D016367:Baculoviridae / Q000235:genetics; D003521:Cyclopropanes / Q000737:chemistry*; D057075:Enzyme Assays; D006147:Guanine / Q000031:analogs & derivatives* / Q000737:chemistry; D015654:Herpesvirus 6, Human / Q000737:chemistry / Q000201:enzymology*; D006801:Humans; D007700:Kinetics; D009705:Nucleosides / Q000737:chemistry*; D010766:Phosphorylation; D011494:Protein Kinases / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D013379:Substrate Specificity; D014764:Viral Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification
chemical_list: D000998:Antiviral Agents; D003521:Cyclopropanes; D009705:Nucleosides; D011993:Recombinant Fusion Proteins; D014764:Viral Proteins; D006147:Guanine; C495219:cyclopropavir; D011494:Protein Kinases
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