title: Critical aspects to be considered prior to large-scale production of peptides.
abstract: Numerous peptides are available on the market as therapeutic drugs for regulating tumor growth, microorganism proliferation, immune response and/or metabolic disorders. Peptides are produced either by chemical synthesis or heterologous expression. Independent of the method chosen, there are challenges to transferring its production from the bench (~mg/L) to the industrial (~g/L) scale. Thus, the main scale-up pitfalls for the two methods of peptide production are reviewed here, including the advantages of each. Moreover, there will be a special focus on the main challenges for large-scale, heterologous production systems. Peptides that are currently available on the market are also described with an emphasis on how their process optimization has been designed in order to develop a cost-effective product.
authors: Grupo de Engenharia Metabólica Aplicada a Bioprocessos, Pós-Graduação em Ciências Genômicas e Biotecnologia, Universidade Católica de Brasília SGAN, Quadra 916, Módulo B, Av. W5 Norte, CEP 70.790-160 Brasília-DF, Brazil. nadiasp@gmail.com.
authors: Kelly Cristina Leite
authors: KC
authors: Mulder
authors: Antônio Américo Barbosa
authors: AA
authors: Viana
authors: Mauro
authors: M
authors: Xavier
authors: Nádia Skorupa
authors: NS
authors: Parachin
mesh_terms: D001786:Blood Glucose / Q000378:metabolism; D001862:Bone Resorption / Q000188:drug therapy; D005818:Genetic Engineering / Q000379:methods*; D013006:Growth Hormone / Q000037:antagonists & inhibitors; D006678:HIV / Q000187:drug effects; D006801:Humans; D010455:Peptides / Q000235:genetics* / Q000378:metabolism* / Q000494:pharmacology / Q000627:therapeutic use
chemical_list: D001786:Blood Glucose; D010455:Peptides; D013006:Growth Hormone