title: A large-scale gene discovery for the red palm weevil Rhynchophorus ferrugineus (Coleoptera: Curculionidae).
abstract: The red palm weevil (RPW; Rhynchophorus ferrugineus) is a devastating pest of palms, prevalent in the Middle East as well as many other regions of the world. Here, we report a large-scale de novo complementary DNA (cDNA) sequencing effort that acquired ∼5 million reads and assembled them into 26 765 contigs from 12 libraries made from samples of different RPW developmental stages based on the Roche/454 GS FLX platform. We annotated these contigs based on the publically available known insect genes and the Tribolium castaneum genome assembly. We find that over 80% of coding sequences (CDS) from the RPW contigs have high-identity homologs to known proteins with complete CDS. Gene expression analysis shows that the pupa and larval stages have the highest and lowest expression levels, respectively. In addition, we also identified more than 60 000 single nucleotide polymorphisms and 1 200 simple sequence repeat markers. This study provides the first large-scale cDNA dataset for RPW, a much-needed resource for future molecular studies.
authors: Joint Center for Genomics Research (JCGR), King Abdulaziz City for Science and Technology (KACST) and Chinese Academy of Sciences (CAS), Riyadh, Kingdom of Saudi Arabia; CAS Key Laboratory of Genome Sciences and Information, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing; Graduate University of Chinese Academy of Sciences, Beijing, China.
authors: Lei
authors: L
authors: Wang
authors: Xiao-Wei
authors: XW
authors: Zhang
authors: Lin-Lin
authors: LL
authors: Pan
authors: Wan-Fei
authors: WF
authors: Liu
authors: Da-Peng
authors: DP
authors: Wang
authors: Guang-Yu
authors: GY
authors: Zhang
authors: Yu-Xin
authors: YX
authors: Yin
authors: An
authors: A
authors: Yin
authors: Shan-Gang
authors: SG
authors: Jia
authors: Xiao-Guang
authors: XG
authors: Yu
authors: Gao-Yuan
authors: GY
authors: Sun
authors: Song-Nian
authors: SN
authors: Hu
authors: Ibrahim S
authors: IS
authors: Al-Mssallem
authors: Jun
authors: J
authors: Yu
mesh_terms: D000818:Animals; D005260:Female; D015723:Gene Library; D017344:Genes, Insect; D054643:INDEL Mutation; D008297:Male; D018895:Microsatellite Repeats; D058977:Molecular Sequence Annotation; D020641:Polymorphism, Single Nucleotide; D017422:Sequence Analysis, DNA; D048889:Weevils / Q000235:genetics* / Q000254:growth & development
keywords: GS FLX Titanium; full-length cDNA; gene expression; pest; red palm weevil