title: Development of Detection Methods for Cellulolytic Activity of Auricularia auricula-judae.
abstract: To obtain basic information on the detection of cellulolytic activity in Auricularia auricula-judae, the influences of dye reagent, pH, and temperature were assessed. Chromogenic dye (congo red, phenol red, remazol brilliant blue, and trypan blue) was individually incorporated into a medium containing either carboxymethyl-cellulose, Avicel, or D-cellobiose as a polysaccharide carbon substrate. The other assessments utilized pHs ranging from 4.5 to 8.0 and temperatures from 15~35℃. Overall, when A. auricula-judae species were transferred onto media contained Congo red and adjusted pH 7.0 and then incubated at 25℃ for 5 days, the clear zone indicative of cellulolytic activity was more pronounced.
authors: Department of Agricultural Environment, Gyeongbuk Agricultural Technology Administration, Daegu 702-320, Korea.
authors: Woo-Sik
authors: WS
authors: Jo
authors: Soon-Hwa
authors: SH
authors: Bae
authors: Seung-Yong
authors: SY
authors: Choi
authors: So-Deuk
authors: SD
authors: Park
authors: Young-Bok
authors: YB
authors: Yoo
authors: Seung-Chun
authors: SC
authors: Park
keywords: Auricularia auricula-judae; Cellulolytic enzyme; Chromogenic media; Congo red
references: Mycobiology. 2007 Mar;35(1):21-4
references: 24015063
references: Mycobiology. 2006 Jun;34(2):108-10
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