title: Tocotrienol Attenuates Stress-Induced Gastric Lesions via Activation of Prostaglandin and Upregulation of COX-1 mRNA.
abstract: The present study aims to distinguish the effect of tocotrienol on an important gastric protective factor, prostaglandin E2 (PGE2), in stress-induced gastric injury. Twenty-eight Wistar rats were divided into four groups of seven rats each. Two control groups were fed commercial rat diet, and two treatment groups were fed the same diet but with additional dose of omeprazole (20 mg/kg) or tocotrienol (60 mg/kg). After 28 days, rats from one control group and both treated groups were subjected to water-immersion restraint stress for 3.5 hours once. The rats were then sacrificed, their stomach isolated and gastric juice collected, lesions examined, and gastric PGE2 content and cyclooxygenase (COX) mRNA expression were determined. Both the regimes significantly attenuated the total lesion area in the stomach compared to the control. Gastric acidity, which was increased in stress, was significantly reduced in rats supplemented with omeprazole and tocotrienol. The PGE2 content was also significantly higher in the rats given tocotrienol supplementation compared to the control followed by an increase in COX-1 mRNA expression. We conclude that tocotrienol supplementation protected rat gastric mucosa against stress-induced lesions possibly by reducing gastric acidity and preserving gastric PGE2 by increasing COX-1 mRNA.
authors: Department of Pharmacology, Faculty of Medicine, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Malaysia.
authors: Mohd Fahami
authors: MF
authors: Nur Azlina
authors: Yusof
authors: Y
authors: Kamisah
authors: Kien Hui
authors: KH
authors: Chua
authors: Hj Mohd Saad
authors: HM
authors: Qodriyah
references: J Biol Chem. 1981 Aug 10;256(15):7730-3
references: 6267023
references: Dig Dis Sci. 1986 Feb;31(2):166-71
references: 3943444
references: Am J Physiol. 1986 Feb;250(2 Pt 1):G191-9
references: 3953798
references: Am J Physiol. 1980 Jul;239(1):G44-8
references: 7396002
references: J Physiol Paris. 2001 Jan-Dec;95(1-6):361-7
references: 11595461
references: Res Commun Mol Pathol Pharmacol. 2003;113-114:39-56
references: 15686106
references: Jpn J Pharmacol. 1999 Jan;79(1):101-7
references: 10082323
references: J Physiol Pharmacol. 2002 Mar;53(1):39-50
references: 11939718
references: Curr Pharm Des. 2003;9(27):2177-90
references: 14529398
references: Free Radic Biol Med. 2011 Jul 1;51(1):129-43
references: 21536125
references: Ann Intern Med. 1985 Aug;103(2):173-7
references: 3874573
references: J Physiol Paris. 1999 Nov;93(5):423-31
references: 10674920
references: Gastroenterology. 1987 Apr;92(4):969-77
references: 3557003
references: Eur J Pharmacol. 1998 Jan 19;342(1):55-65
references: 9544793
references: Aliment Pharmacol Ther. 1997 Dec;11(6):1101-8
references: 9663836
references: Aliment Pharmacol Ther. 2002 Apr;16 Suppl 2:29-34
references: 11966521
references: World J Gastroenterol. 2011 Feb 7;17(5):657-61
references: 21350716
references: Clin Exp Pharmacol Physiol. 1997 Nov;24(11):844-9
references: 9363367
references: J Clin Gastroenterol. 1990;12 Suppl 1:S48-51
references: 2212549
references: N Engl J Med. 1974 Oct 31;291(18):925-9
references: 4547133
references: Methods Enzymol. 1994;234:354-66
references: 7808307
references: Hepatogastroenterology. 2004 Nov-Dec;51(60):1626-30
references: 15532792
references: Regul Pept. 2008 Jun 5;148(1-3):6-20
references: 18378017
references: Food Funct. 2012 Jan;3(1):83-90
references: 22038069
references: Am J Physiol Gastrointest Liver Physiol. 2003 Aug;285(2):G414-23
references: 12686508
references: BMC Gastroenterol. 2012 May 28;12:54
references: 22639913
references: Gastroenterology. 1954 Jun;26(6):906-13
references: 13162085
references: Br J Pharmacol. 2006 Nov;149(6):611-23
references: 17016496
references: Digestion. 1982;25(3):155-63
references: 6761220
references: Am J Physiol Gastrointest Liver Physiol. 2003 Dec;285(6):G1214-24
references: 12893632
references: J Biomol Screen. 2005 Sep;10(6):549-56
references: 16103413
references: Dig Dis Sci. 2007 Feb;52(2):478-87
references: 17226073
references: Clin Chim Acta. 2004 Feb;340(1-2):229-33
references: 14734217
references: Best Pract Res Clin Gastroenterol. 2003 Jun;17(3):327-44
references: 12763499
references: Gastroenterology. 1981 Apr;80(4):770-5
references: 6162705
references: Curr Mol Med. 2008 Jun;8(4):253-7
references: 18537633
references: Ann Surg. 1989 Nov;210(5):590-5
references: 2510618
references: J Pharmacol Exp Ther. 2006 Feb;316(2):547-55
references: 16236816