title: Rapidly developing yeast microcolonies differentiate in a similar way to aging giant colonies.
abstract: During their development and aging on solid substrates, yeast giant colonies produce ammonia, which acts as a quorum sensing molecule. Ammonia production is connected with alkalization of the surrounding medium and with extensive reprogramming of cell metabolism. In addition, ammonia signaling is important for both horizontal (colony centre versus colony margin) and vertical (upper versus lower cell layers) colony differentiations. The centre of an aging differentiated giant colony is thus composed of two major cell subpopulations, the subpopulation of long-living, metabolically active and stress-resistant cells that form the upper layers of the colony and the subpopulation of stress-sensitive starving cells in the colony interior. Here, we show that microcolonies originating from one cell pass through similar developmental phases as giant colonies. Microcolony differentiation is linked to ammonia signaling, and cells similar to the upper and lower cells of aged giant colonies are formed even in relatively young microcolonies. A comparison of the properties of these cells revealed a number of features that are similar in microcolonies and giant colonies as well as a few that are only typical of chronologically aged giant colonies. These findings show that colony age per se is not crucial for colony differentiation.
authors: Institute of Microbiology of the ASCR, v.v.i., 142 20 Prague 4, Czech Republic.
authors: Libuše
authors: L
authors: Váchová
authors: Ladislava
authors: L
authors: Hatáková
authors: Michal
authors: M
authors: Cáp
authors: Michaela
authors: M
authors: Pokorná
authors: Zdena
authors: Z
authors: Palková
mesh_terms: D000641:Ammonia / Q000378:metabolism; D015398:Signal Transduction / Q000502:physiology*; D015003:Yeasts / Q000378:metabolism*
chemical_list: D000641:Ammonia
references: J Biol Chem. 2009 Nov 20;284(47):32572-81
references: 19801643
references: Appl Environ Microbiol. 2010 Jun;76(12):4089-91
references: 20435772
references: Yeast. 2004 Aug;21(11):947-62
references: 15334558
references: PLoS One. 2012;7(9):e46243
references: 23029448
references: EMBO Rep. 2004 May;5(5):470-6
references: 15184977
references: Science. 2001 Feb 2;291(5505):878-81
references: 11157168
references: J Bacteriol. 1998 Aug;180(15):3992-6
references: 9683500
references: Mol Cell Biol. 2001 Jul;21(13):4347-68
references: 11390663
references: Environ Microbiol. 2009 Feb;11(2):494-504
references: 19196279
references: Antonie Van Leeuwenhoek. 1969 Jun;35:Suppl:F35-6
references: 5311988
references: PLoS Genet. 2010 Jan 22;6(1):e1000823
references: 20107600
references: Microbiology (Reading). 2009 Dec;155(Pt 12):3838-3846
references: 19729408
references: Genetics. 2010 Mar;184(3):707-16
references: 20038633
references: PLoS Pathog. 2012 Jan;8(1):e1002485
references: 22253597
references: Mol Cell. 2012 May 25;46(4):436-48
references: 22560924
references: Biochem Soc Trans. 2011 Oct;39(5):1471-5
references: 21936836
references: FEMS Microbiol Rev. 2006 Sep;30(5):806-24
references: 16911045
references: J Cell Sci. 2000 Jun;113 ( Pt 11):1923-8
references: 10806103
references: Environ Microbiol. 2009 Jul;11(7):1866-77
references: 19302539
references: Curr Opin Microbiol. 2013 Aug;16(4):398-403
references: 23566895
references: Mol Biol Cell. 2002 Nov;13(11):3901-14
references: 12429834
references: J Cell Biol. 2005 Jun 6;169(5):711-7
references: 15939758
references: J Cell Biol. 2011 Sep 5;194(5):679-87
references: 21875945
references: Infect Immun. 1994 Mar;62(3):915-21
references: 8112864
references: J Biol Chem. 2000 May 12;275(19):14408-14
references: 10799523
references: Mol Microbiol. 2003 Feb;47(3):745-54
references: 12535073
references: J Biol Chem. 2004 Sep 3;279(36):37973-81
references: 15229222
references: Nature. 1997 Dec 4;390(6659):532-6
references: 9394006