title: Validation of blood and liver oxymorphone analysis using LC-MS-MS: concentrations in 30 fatal overdoses.
abstract: A sensitive liquid chromatography-tandem mass spectrometry method was developed and validated for the quantitation of oxymorphone (OM) in human whole blood and liver. Sample preparation was done by solid-phase extraction, using deuterated OM as the internal standard. Separation was achieved using a Waters Aquity UPLC HSS T3 column. Analysis utilized positive electrospray ionization and multiple reaction monitoring. As part of the validation, studies were conducted to determine potential interference, selectivity, ion suppression/enhancement and carryover. Calibration model, limit of detection (LOD), lower limit of quantitation (LLOQ), precision and accuracy were also established. The linear range of the method was 2-500 ng/mL in blood and 5-500 ng/g in the liver. The LOD and LLOQ were 2 ng/mL for blood and 5 ng/g for the liver. Blood and/or liver specimens from 30 cases were analyzed. OM concentrations ranged from 23 to 554 ng/mL ( , n = 26) in blood and 48 to 1740 ng/g ( , n = 30) in the liver.
authors: 1 Marshall University, 1401 Forensic Science Drive, Huntington, WV 25701, USA.
authors: Erin D
authors: ED
authors: Crum
authors: Kristen M
authors: KM
authors: Bailey
authors: Lauren L
authors: LL
authors: Richards-Waugh
authors: David J
authors: DJ
authors: Clay
authors: Myron A
authors: MA
authors: Gebhardt
authors: James C
authors: JC
authors: Kraner
mesh_terms: D000328:Adult; D001344:Autopsy; D002138:Calibration; D002423:Cause of Death; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D062787:Drug Overdose / Q000097:blood* / Q000378:metabolism / Q000401:mortality; D005260:Female; D006801:Humans; D057230:Limit of Detection; D016014:Linear Models; D008099:Liver / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D010111:Oxymorphone / Q000097:blood* / Q000493:pharmacokinetics / Q000506:poisoning; D015203:Reproducibility of Results; D052616:Solid Phase Extraction; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014018:Tissue Distribution; D055815:Young Adult
chemical_list: D010111:Oxymorphone