title: Heritable custom genomic modifications in Caenorhabditis elegans via a CRISPR-Cas9 system.
abstract: We adapted the CRISPR-Cas9 system for template-mediated repair of targeted double-strand breaks via homologous recombination in Caenorhabditis elegans, enabling customized and efficient genome editing. This system can be used to create specific insertions, deletions, and base pair changes in the germline of C. elegans.
authors: Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115.
authors: Yonatan B
authors: YB
authors: Tzur
authors: Ari E
authors: AE
authors: Friedland
authors: Saravanapriah
authors: S
authors: Nadarajan
authors: George M
authors: GM
authors: Church
authors: John A
authors: JA
authors: Calarco
authors: Monica P
authors: MP
authors: Colaiácovo
mesh_terms: D000818:Animals; D064113:CRISPR-Cas Systems; D017173:Caenorhabditis elegans / Q000235:genetics*; D053903:DNA Breaks, Double-Stranded; D018509:DNA, Helminth / Q000235:genetics; D018390:Gene Targeting; D005818:Genetic Engineering; D049751:Genome, Helminth; D059765:Homologous Recombination; D016296:Mutagenesis; D016254:Mutagenesis, Insertional; D017393:RNA Editing / Q000235:genetics
chemical_list: D018509:DNA, Helminth
keywords: CRISPR–Cas9; Caenorhabditis elegans; genome editing; germline; homologous recombination
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