title: The first latent green fluorophores for the detection of azoreductase activity in bacterial cultures.
abstract: An efficient synthesis of original bio-reductive probes suitable for the detection of azoreductases from the fluorescent rhodamine 110 dye is presented. A "turn-on" green fluorescence response upon reduction of the two diazo bonds of these latent fluorophores was observed both in vitro and in the context of bacterial cultures.
authors: Normandie Univ, COBRA, UMR 6014 & FR 3038, UNIV Rouen, INSA Rouen, CNRS, 1 Rue Tesnières, 76821 Mont-Saint-Aignan Cedex, France. anthony.romieu@univ-rouen.fr pierre-yves.renard@univ-rouen.fr.
authors: Arnaud
authors: A
authors: Chevalier
authors: Claire
authors: C
authors: Mercier
authors: Laura
authors: L
authors: Saurel
authors: Sylvain
authors: S
authors: Orenga
authors: Pierre-Yves
authors: PY
authors: Renard
authors: Anthony
authors: A
authors: Romieu
mesh_terms: D001391:Azo Compounds / Q000032:analysis / Q000378:metabolism; D001419:Bacteria / Q000201:enzymology*; D057075:Enzyme Assays; D005456:Fluorescent Dyes / Q000032:analysis / Q000378:metabolism*; D008958:Models, Molecular; D009247:NADH, NADPH Oxidoreductases / Q000032:analysis / Q000378:metabolism*; D009601:Nitroreductases; D010084:Oxidation-Reduction; D012235:Rhodamines / Q000032:analysis / Q000378:metabolism*
chemical_list: D001391:Azo Compounds; D005456:Fluorescent Dyes; D012235:Rhodamines; C049025:rhodamine 110; D009247:NADH, NADPH Oxidoreductases; D009601:Nitroreductases; C021106:azoreductase