title: Extremely low-frequency electromagnetic fields induce neural differentiation in bone marrow derived mesenchymal stem cells.
abstract: Extremely low-frequency electromagnetic fields (ELF-EMF) affect numerous biological functions such as gene expression, cell fate determination and even cell differentiation. To investigate the correlation between ELF-EMF exposure and differentiation, bone marrow derived mesenchymal stem cells (BM-MSCs) were subjected to a 50-Hz electromagnetic field during in vitro expansion. The influence of ELF-EMF on BM-MSCs was analysed by a range of different analytical methods to understand its role in the enhancement of neural differentiation. ELF-EMF exposure significantly decreased the rate of proliferation, which in turn caused an increase in neuronal differentiation. The ELF-EMF-treated cells showed increased levels of neuronal differentiation marker (MAP2), while early neuronal marker (Nestin) was down-regulated. In addition, eight differentially expressed proteins were detected in two-dimensional electrophoresis maps, and were identified using ESI-Q-TOF LC/MS/MS. Among them, ferritin light chain, thioredoxin-dependent peroxide reductase, and tubulin β-6 chain were up-regulated in the ELF-EMF-stimulated group. Ferritin and thioredoxin-dependent peroxide reductase are involved in a wide variety of functions, including Ca(2+) regulation, which is a critical component of neurodegeneration. We also observed that the intracellular Ca(2+) content was significantly elevated after ELF-EMF exposure, which strengthens the modulatory role of ferritin and thioredoxin-dependent peroxide reductase, during differentiation. Notably, western blot analysis indicated significantly increased expression of the ferritin light chain in the ELF-EMF-stimulated group (0.60 vs. 1.08; P < 0.01). These proteins may help understand the effect of ELF-EMF stimulation on BM-MSCs during neural differentiation and its potential use as a clinically therapeutic option for treating neurodegenerative diseases.
authors: School of Life Sciences and Biotechnology, Korea University, Seoul 136-701, Korea.
authors: Hyun-Jung
authors: HJ
authors: Kim
authors: Jessica
authors: J
authors: Jung
authors: Jee-Hye
authors: JH
authors: Park
authors: Jin-Hee
authors: JH
authors: Kim
authors: Kyung-Nam
authors: KN
authors: Ko
authors: Chan-Wha
authors: CW
authors: Kim
mesh_terms: D001854:Bone Marrow Cells; D002118:Calcium / Q000502:physiology; D002454:Cell Differentiation / Q000502:physiology*; D002460:Cell Line; D049109:Cell Proliferation; D015536:Down-Regulation / Q000502:physiology; D004574:Electromagnetic Fields; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000502:physiology; D008869:Microtubule-Associated Proteins / Q000502:physiology; D064231:Nestin / Q000502:physiology; D009474:Neurons / Q000166:cytology* / Q000502:physiology; D015854:Up-Regulation / Q000502:physiology
chemical_list: C489788:MAP2 protein, human; D008869:Microtubule-Associated Proteins; D064231:Nestin; D002118:Calcium
keywords: BM-MSCs; Ca2+ regulation; Extremely low-frequency electromagnetic fields; ferritin; neural differentiation