title: Biodegradation of 4-bromophenol by Arthrobacter chlorophenolicus A6 in batch shake flasks and in a continuously operated packed bed reactor.
abstract: The present study investigated growth and biodegradation of 4-bromophenol (4-BP) by Arthrobacter chlorophenolicus A6 in batch shake flasks as well as in a continuously operated packed bed reactor (PBR). Batch growth kinetics of A. chlorophenolicus A6 in presence of 4-BP followed substrate inhibition kinetics with the estimated biokinetic parameters value of μ max = 0.246 h(-1), K i = 111 mg L(-1), K s  = 30.77 mg L(-1) and K = 100 mg L(-1). In addition, variations in the observed and theoretical biomass yield coefficient and maintenance energy of the culture were investigated at different initial 4-BP concentration. Results indicates that the toxicity tolerance and the biomass yield of A. chlorophenolicus A6 towards 4-BP was found to be poor as the organism utilized the substrate mainly for its metabolic maintenance energy. Further, 4-BP biodegradation performance by the microorganism was evaluated in a continuously operated PBR by varying the influent concentration and hydraulic retention time in the ranges 400-1,200 mg L(-1) and 24-7.5 h, respectively. Complete removal of 4-BP was achieved in the PBR up to a loading rate of 2,276 mg L(-1) day(-1).
authors: Environmental Science and Technology Section (ITER), Department of Chemistry, S'O'A University, Bhubaneswar, 751030, Orissa, India, nareshs2010@gmail.com.
authors: Naresh Kumar
authors: NK
authors: Sahoo
authors: Kannan
authors: K
authors: Pakshirajan
authors: Pranab Kumar
authors: PK
authors: Ghosh
mesh_terms: D001173:Arthrobacter / Q000737:chemistry / Q000254:growth & development / Q000378:metabolism*; D061249:Batch Cell Culture Techniques; D001673:Biodegradation, Environmental; D019149:Bioreactors / Q000382:microbiology*; D007700:Kinetics; D010636:Phenols / Q000737:chemistry / Q000378:metabolism*
chemical_list: D010636:Phenols; C032037:4-bromophenol