title: Detection in vitro and quantitative estimation of artificial microterritories which promote osteogenic differentiation and maturation of stromal stem cells.
abstract: Extracellular matrix can regulate multipotent mesenchymal stromal cells (MMSC) differentiation, with potential applications for tissue engineering. A relief of mineralized bone takes essential effect on MMSC fate. Nevertheless, delicate structure and a hierarchy of niches for stromal stem cells and its quantitative parameters are not practically known. Here, we describe the protocol to define the basic approach providing a guiding for in vitro identification of quantitative features of artificial calcium phosphate niches which promotes osteogenic differentiation and maturation of stromal stem cell.
authors: Scientific Educational Center, Biocompatible Materials and Bioengineering, Siberian State Medical University, Tomsk, Russia.
authors: Igor A
authors: IA
authors: Khlusov
authors: Natalya M
authors: NM
authors: Shevtsova
authors: Marina Yu
authors: MY
authors: Khlusova
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D002130:Calcium Phosphates / Q000737:chemistry; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D002478:Cells, Cultured; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D008168:Lung / Q000166:cytology; D059630:Mesenchymal Stem Cells / Q000502:physiology*; D008855:Microscopy, Electron, Scanning; D015675:Osteocalcin / Q000378:metabolism; D010012:Osteogenesis; D055153:Stem Cell Niche; D013499:Surface Properties; D016707:Tissue Fixation
chemical_list: D002130:Calcium Phosphates; D015675:Osteocalcin; C020243:calcium phosphate; D000469:Alkaline Phosphatase