title: A proteorhodopsin-based biohybrid light-powering pH sensor.
abstract: The biohybrid sensor is an emerging technique for multi-functional detection that utilizes the instinctive responses or interactions of biomolecules. We develop a biohybrid pH sensor by taking advantage of the pH-dependent photoelectric characteristics of proteorhodopsin (pR). The transient absorption kinetics study indicates that the photoelectric behavior of pR is attributed to the varying lifetime of the M intermediate at different environmental pH values. This pR-based biohybrid light-powering sensor with microfluidic design can achieve real-time pH detection with quick response and high sensitivity. The results of this work would shed light on pR and its potential applications.
authors: Key Laboratory of Bio-Inspired Smart Interfacial Science and Technology of Ministry of Education, School of Chemistry and Environment, Beihang University, Beijing, 100191, P.R. China.
authors: Siyuan
authors: S
authors: Rao
authors: Zhibin
authors: Z
authors: Guo
authors: Dawei
authors: D
authors: Liang
authors: Deliang
authors: D
authors: Chen
authors: Yen
authors: Y
authors: Wei
authors: Yan
authors: Y
authors: Xiang
mesh_terms: D004566:Electrodes; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008027:Light; D046210:Microfluidic Analytical Techniques; D010713:Phosphatidylcholines / Q000737:chemistry; D012243:Rhodopsin / Q000737:chemistry*; D025604:Rhodopsins, Microbial; D017971:Tin Compounds / Q000737:chemistry
chemical_list: D010713:Phosphatidylcholines; D025604:Rhodopsins, Microbial; D017971:Tin Compounds; C413548:proteorhodopsin; C109984:indium tin oxide; D012243:Rhodopsin