title: Adhesion, proliferation, and differentiation of mesenchymal stem cells on RGD nanopatterns of varied nanospacings.
abstract: The present report is an extension of our preceding publication in Biomaterials (2013) entitled "Effect of RGD nanospacing on differentiation of stem cells." Cell-adhesive peptide arginine-glycine-aspartate (RGD) was nanopatterned on a non-fouling poly(ethylene glycol) (PEG) hydrogel, and mesenchymal stem cells (MSCs) derived from rat bone marrow were cultured on the patterned surfaces at nanospacings from 37 to 124 nm. Cell adhesion parameters such as spreading areas varied with RGD nanospacings significantly. The differences were well observed at both the first and eighth days, which confirmed the persistence of this nanospacing effect on our nanopatterns. The proliferation rate also varied with the nanospacings. Osteogenic and adipogenic inductions were undertaken, and a significant influence of RGD nanospacing on stem cell differentiation was found. The effect on differentiation cannot be simply interpreted by differences in cell adhesion and proliferation. We further calculated the fractions of single, coupled, and multiple cells on those nanopatterns, and ruled out the possibility that the extent of cell-cell contact determined the different differentiation fractions. Accordingly, we reinforced the idea that RGD nanospacing might directly influence stem cell differentiation.
authors: State Key Laboratory of Molecular Engineering of Polymers; Department of Macromolecular Science; Fudan University; Shanghai, PR China.
authors: Xuan
authors: X
authors: Wang
authors: State Key Laboratory of Molecular Engineering of Polymers; Department of Macromolecular Science; Fudan University; Shanghai, PR China.
authors: Kai
authors: K
authors: Ye
authors: State Key Laboratory of Molecular Engineering of Polymers; Department of Macromolecular Science; Fudan University; Shanghai, PR China.
authors: Zhenhua
authors: Z
authors: Li
authors: State Key Laboratory of Molecular Engineering of Polymers; Department of Macromolecular Science; Fudan University; Shanghai, PR China.
authors: Ce
authors: C
authors: Yan
authors: State Key Laboratory of Molecular Engineering of Polymers; Department of Macromolecular Science; Fudan University; Shanghai, PR China.
authors: Jiandong
authors: J
authors: Ding
mesh_terms: D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D002450:Cell Communication / Q000187:drug effects; D002452:Cell Count; D002454:Cell Differentiation / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D006046:Gold / Q000494:pharmacology; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000494:pharmacology; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000648:ultrastructure; D018625:Microscopy, Atomic Force; D008856:Microscopy, Fluorescence; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D009842:Oligopeptides / Q000494:pharmacology*; D011092:Polyethylene Glycols / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D009842:Oligopeptides; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D011092:Polyethylene Glycols; D006046:Gold; C047981:arginyl-glycyl-aspartic acid
keywords: PEG hydrogel; RGD; cell adhesion; cell differentiation; cell proliferation; nanopattern; stem cell
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