title: Rhodamine-propargylic esters for detection of mitochondrial hydrogen sulfide in living cells.
abstract: Flow cytometric detection of mitochondrial H2S was achieved with propargylic esters of rhodamine B which selectively react with H2S via cationic rhodamine-moiety directed thiolysis of the propargylic esters to give nonfluorescent rhodamine thio-spirolactone.
authors: Department of Chemical Biology, College of Chemistry and Chemical Engineering and the Key Laboratory for Chemical Biology of Fujian Province, Xiamen University, Xiamen, China.
authors: Xi
authors: X
authors: Chen
authors: Shuqi
authors: S
authors: Wu
authors: Jiahuai
authors: J
authors: Han
authors: Shoufa
authors: S
authors: Han
mesh_terms: D000480:Alkynes / Q000737:chemistry; D004952:Esters / Q000737:chemistry*; D005434:Flow Cytometry; D005456:Fluorescent Dyes / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D006862:Hydrogen Sulfide / Q000032:analysis*; D018613:Microscopy, Confocal; D008928:Mitochondria / Q000737:chemistry*; D015394:Molecular Structure; D020005:Propanols / Q000737:chemistry; D012235:Rhodamines / Q000737:chemistry*
chemical_list: D000480:Alkynes; D004952:Esters; D005456:Fluorescent Dyes; D020005:Propanols; D012235:Rhodamines; C028255:propargyl alcohol; D006862:Hydrogen Sulfide
keywords: Bioimaging; Flow cytometry; Hydrogen sulfide; Mitochondria; Propargylic ester; Rhodamine