title: Identification and separation of normal hematopoietic stem cells and leukemia stem cells from patients with acute myeloid leukemia.
abstract: Mounting evidences indicate that leukemic cells in patients with acute myeloid leukemia (AML) are derived from leukemia stem cells (LSC). In analogy to normal hematopoietic stem cells (HSC), LSC remain mostly dormant and are hence resistant to conventional chemotherapy. Residual, physiological HSC exist alongside with LSC, with heterogeneous dominance of LSC over HSC in individual patients. We have devised a flow cytometric method for the identification and separation of these two stem cell populations based on surface antigen markers such as CD34, CD38, lineage aberrant markers, and aldehyde dehydrogenase (ALDH) enzyme activity.
authors: Department of Internal Medicine V, University of Heidelberg, Heidelberg, Germany.
authors: Van T
authors: VT
authors: Hoang
authors: Isabel
authors: I
authors: Hoffmann
authors: Karina
authors: K
authors: Borowski
authors: Abraham
authors: A
authors: Zepeda-Moreno
authors: Dan
authors: D
authors: Ran
authors: Eike C
authors: EC
authors: Buss
authors: Patrick
authors: P
authors: Wuchter
authors: Volker
authors: V
authors: Eckstein
authors: Anthony D
authors: AD
authors: Ho
mesh_terms: D051997:ADP-ribosyl Cyclase 1 / Q000378:metabolism; D000444:Aldehyde Dehydrogenase / Q000378:metabolism; D018952:Antigens, CD34 / Q000378:metabolism; D002469:Cell Separation; D005434:Flow Cytometry; D006412:Hematopoietic Stem Cells / Q000378:metabolism*; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000473:pathology*; D008562:Membrane Glycoproteins / Q000378:metabolism; D014411:Neoplastic Stem Cells / Q000378:metabolism*
chemical_list: D018952:Antigens, CD34; D008562:Membrane Glycoproteins; D000444:Aldehyde Dehydrogenase; C498154:CD38 protein, human; D051997:ADP-ribosyl Cyclase 1