title: Jumping and gliding rodents: mitogenomic affinities of Pedetidae and Anomaluridae deduced from an RNA-Seq approach.
abstract: An RNA-Seq strategy was used to obtain the complete set of protein-coding mitochondrial genes from two rodent taxa. Thanks to the next generation sequencing (NGS) 454 approach, we determined the complete mitochondrial DNA genome from Graphiurus kelleni (Mammalia: Rodentia: Gliridae) and partial mitogenome from Pedetes capensis (Pedetidae), and compared them with published rodent and outgroup mitogenomes. We finished the mitogenome sequencing by a series of amplicons using conserved PCR primers to fill the gaps corresponding to tRNA, rRNA and control regions. Phylogenetic analyses of the mitogenomes suggest a well-supported rodent phylogeny in agreement with nuclear gene trees. Pedetes groups with Anomalurus into the clade Anomaluromorpha, while Graphiurus branches within the squirrel-related clade. Moreover, Pedetes+Anomalurus branch with Castor into the mouse-related clade. Our study demonstrates the utility of NGS for obtaining new mitochondrial genomes as well as the importance of choosing adequate models of sequence evolution to infer the phylogeny of rodents.
authors: Institut des Sciences de l'Evolution (ISEM, UMR 5554 UM2-CNRS-IRD), Université Montpellier II, Place Eugène Bataillon - CC 064 - 34095 Montpellier Cedex 5, France; Center for Macroecology Evolution and Climate at the Natural History Museum of Denmark, University of Copenhagen, Universitetsparken, 15, DK-2100 Copenhagen Ø, Denmark.
authors: Pierre-Henri
authors: PH
authors: Fabre
authors: Knud A
authors: KA
authors: Jønsson
authors: Emmanuel J P
authors: EJ
authors: Douzery
mesh_terms: D000818:Animals; D001499:Bayes Theorem; D001522:Behavior, Animal / Q000502:physiology; D004272:DNA, Mitochondrial / Q000235:genetics; D019143:Evolution, Molecular; D054629:Genome, Mitochondrial / Q000235:genetics*; D059014:High-Throughput Nucleotide Sequencing; D016013:Likelihood Functions; D051379:Mice; D009043:Motor Activity / Q000235:genetics*; D010802:Phylogeny; D012335:RNA, Ribosomal / Q000032:analysis / Q000235:genetics*; D012377:Rodentia / Q000033:anatomy & histology / Q000145:classification / Q000235:genetics* / Q000502:physiology*; D017423:Sequence Analysis, RNA / Q000379:methods
chemical_list: D004272:DNA, Mitochondrial; D012335:RNA, Ribosomal
keywords: AIC; AU test; Akaike Information Criterion; Approximately Unbiased test; BP; Bootstrap Percentage; GTR; General Time Reversible; Graphiurus; ML; Maximum Likelihood; Mitochondrial DNA; NGS; Next Generation Sequencing; Next generation sequencing; PP; Pedetes; Phylogeny; Posterior Probability; RNA Sequencing; RNA-Seq; Rodentia