title: Generation of porcine induced pluripotent stem cells and evaluation of their major histocompatibility complex protein expression in vitro.
abstract: Induced pluripotent stem cells (iPSCs) are thought to be highly beneficial in the field of regenerative medicine and are believed to overcome immunogenic barriers to cell transplantation. However, issues remain regarding their safety and efficiency for medical use. Furthermore, some recent reports have suggested that iPSCs could be targeted by the autologous immune system. To promote practical applications of iPSCs, in depth research using appropriate animal models is needed and porcine species appear to provide an ideal model. Recent studies have focused on the generation of porcine iPSC cells, but no investigations of their immunological properties have been conducted to date. In the present study, we generated putative iPSCs from porcine somatic cells and measured major histocompatibility complex (MHC) expression on the iPSCs and their derivatives. Compact colonies that expressed pluripotent markers appeared 11 days after viral infection. Embryonic bodies (EB) were produced and differentiated into three germ layers in vitro. Karyotyping and swine leukocyte antigen (SLA) typing showed that the iPSCs were identical to parental somatic cells. Porcine iPSCs expressed only low levels of MHC class I and moderately increased levels on their differentiated derivatives, whereas MHC class II was rarely expressed. In the presence of interferon-gamma (IFN-γ), the expression of MHC class I was elevated on differentiated iPSCs, and gradually decreased after withdrawal of the cytokine. Our data suggest that porcine iPSCs could be useful for preclinical studies of the efficiency and viability of iPSCs, and for devising strategies to rescue transplanted cells from the autologous immune system.
authors: Stem Cell Institute-KNU, Kangwon National University, Chuncheon, Republic of Korea, 200-701.
authors: Kyung-Mee
authors: KM
authors: Park
authors: Sang-Ho
authors: SH
authors: Cha
authors: Cheol
authors: C
authors: Ahn
authors: Heung-Myong
authors: HM
authors: Woo
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology; D002454:Cell Differentiation; D005786:Gene Expression Regulation / Q000187:drug effects; D005819:Genetic Markers / Q000235:genetics; D005838:Genotype; D057026:Induced Pluripotent Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000502:physiology*; D007371:Interferon-gamma / Q000494:pharmacology; D059785:Karyotype; D008285:Major Histocompatibility Complex / Q000235:genetics*; D013552:Swine
chemical_list: D000998:Antiviral Agents; D005819:Genetic Markers; D007371:Interferon-gamma
references: Stem Cells Dev. 2010 Aug;19(8):1211-20
references: 20380514
references: Science. 2007 Dec 21;318(5858):1920-3
references: 18063756
references: Arthritis Res. 2001;3(3):136-41
references: 11299053
references: Stem Cells. 2006 Feb;24(2):221-9
references: 16109762
references: Cell Res. 2009 Nov;19(11):1233-42
references: 19736565
references: Zygote. 2010 May;18(2):93-101
references: 20307349
references: BMC Genomics. 2005 May 10;6:70
references: 15885146
references: Anim Biotechnol. 2005;16(2):183-90
references: 16342425
references: J Biol Chem. 2009 Jun 26;284(26):17634-40
references: 19376775
references: Immunol Invest. 1985 Dec;14(6):473-7
references: 3830869
references: PLoS One. 2010 Apr 16;5(4):e10192
references: 20419139
references: Immunol Today. 1990 Jul;11(7):237-44
references: 2201309
references: Hepatology. 2010 Jan;51(1):297-305
references: 19998274
references: Int J Dev Biol. 2010;54(11-12):1703-11
references: 21305472
references: Nat Protoc. 2008;3(7):1180-6
references: 18600223
references: J Leukoc Biol. 2011 Jun;89(6):855-71
references: 21233410
references: Appl Biochem Biotechnol. 2009 Jun;157(3):538-44
references: 19089654
references: Proc Natl Acad Sci U S A. 2009 Jan 20;106(3):808-13
references: 19139414
references: Proc Natl Acad Sci U S A. 2002 Jul 23;99(15):9864-9
references: 12114532
references: J Bone Miner Res. 2008 Feb;23(2):253-9
references: 18197754
references: Semin Immunopathol. 2011 Nov;33(6):525-34
references: 21461719
references: Transplantation. 2009 May 15;87(9):1300-4
references: 19424028
references: Tissue Antigens. 2009 Apr;73(4):307-15
references: 19317739
references: Tissue Antigens. 2005 Dec;66(6):623-39
references: 16305679
references: Proc Natl Acad Sci U S A. 2009 Jul 7;106(27):10993-8
references: 19541600
references: Cell Cycle. 2009 Oct 1;8(19):3078-81
references: 19738434
references: Cell Res. 2009 Apr;19(4):429-38
references: 19255591
references: J Cardiovasc Transl Res. 2011 Apr;4(2):121-30
references: 21088946
references: J Clin Invest. 2010 Jan;120(1):51-9
references: 20051636
references: Diabetes. 1997 May;46(5):770-8
references: 9133543
references: J Mol Cell Biol. 2009 Oct;1(1):46-54
references: 19502222
references: Nature. 2011 May 13;474(7350):212-5
references: 21572395
references: Tissue Antigens. 2011 Jun;77(6):572-83
references: 21457149
references: Curr Protoc Stem Cell Biol. 2012;Chapter 4:Unit4A.5
references: 22605648
references: Thromb Haemost. 2010 Jul;104(1):39-44
references: 20539907
references: Sci China C Life Sci. 2009 Jul;52(7):622-36
references: 19641867
references: Proc Natl Acad Sci U S A. 2008 Apr 15;105(15):5856-61
references: 18391196
references: Front Biosci. 2007 Sep 01;12:4864-80
references: 17569616
references: IUBMB Life. 2010 Apr;62(4):277-82
references: 20101630
references: Biochem Biophys Res Commun. 2004 Apr 9;316(3):924-9
references: 15033490
references: FASEB J. 2010 Jul;24(7):2164-77
references: 20145206
references: Tissue Antigens. 2005 Feb;65(2):136-49
references: 15713212