title: The effect of low-level laser therapy on the healing of hard palate mucosa and the oxidative stress status of rats.
abstract: OBJECTIVE
  The biostimulation effects of low-level laser therapy (LLLT) have been demonstrated recently. This study investigated the effects of LLLT on palatal mucoperiosteal wound healing and oxidative stress status in rats.
  
  
  METHODS
  Forty-two male Wistar rats weighing 250-300 g were used in this study. A standardized full-thickness wound was created in the mucoperiosteum of the hard palates of the rats using a 3-mm-diameter biopsy punch. Treatment using a GaAlAs laser at a wavelength of 940 nm and a dose of 10 J/cm(2) was initiated after surgery and repeated on the 2nd, 4th, and 6th days post-surgery. Seven animals from each group were sacrificed on the 7th, 14th, and 21st days after surgery. Total antioxidant status and total oxidative status were measured in serum.
  
  
  RESULTS
  The histopathological findings revealed reduced numbers of inflammatory cells on the 7th day, increased mitotic activity of fibroblasts on the 14th and 21st day, and the same degree of collagen synthesis and vascularization on the days 7, 14, and 21 in the LLLT group compared with the control group. No significant differences in total oxidative status and total antioxidant status were observed between the groups.
  
  
  CONCLUSIONS
  LLLT using a GaAlAs laser at a wavelength of 940 nm and a dose of 10 J/cm(2) elicited a positive healing effect on palatal mucoperiosteal wounds likely via the induction of fibroblasts. The oxidative stress status was not affected by LLLT.
authors: Department of Periodontology, Faculty of Dentistry, Dicle University, Diyarbakır, Turkey.
authors: Ela Tules
authors: ET
authors: Firat
authors: Ahmet
authors: A
authors: Dağ
authors: Ahmet
authors: A
authors: Günay
authors: Beyza
authors: B
authors: Kaya
authors: Mehmet İrfan
authors: Mİ
authors: Karadede
authors: Berna
authors: B
authors: Ersöz Kanay
authors: Aydın
authors: A
authors: Ketani
authors: Osman
authors: O
authors: Evliyaoğlu
authors: Ersin
authors: E
authors: Uysal
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000032:analysis; D052160:Benzothiazoles; D001707:Biopsy, Needle / Q000295:instrumentation; D003094:Collagen / Q000528:radiation effects; D004848:Epithelium / Q000473:pathology / Q000528:radiation effects; D005347:Fibroblasts / Q000473:pathology / Q000528:radiation effects; D007202:Indicators and Reagents; D015603:Keratinocytes / Q000473:pathology / Q000528:radiation effects; D054023:Lasers, Semiconductor / Q000627:therapeutic use; D028022:Low-Level Light Therapy / Q000379:methods*; D008297:Male; D008938:Mitosis / Q000528:radiation effects; D009061:Mouth Mucosa / Q000293:injuries / Q000473:pathology / Q000528:radiation effects*; D018919:Neovascularization, Physiologic / Q000528:radiation effects; D009504:Neutrophils / Q000473:pathology / Q000528:radiation effects; D018384:Oxidative Stress / Q000528:radiation effects*; D021362:Palate, Hard / Q000293:injuries / Q000473:pathology / Q000528:radiation effects*; D011879:Radiotherapy Dosage; D051381:Rats; D017208:Rats, Wistar; D013451:Sulfonic Acids; D013997:Time Factors; D014945:Wound Healing / Q000528:radiation effects
chemical_list: D000975:Antioxidants; D052160:Benzothiazoles; D007202:Indicators and Reagents; D013451:Sulfonic Acids; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; D003094:Collagen
keywords: biostimulation; hard palate; low-level laser therapy; oxidative stress; wound healing