title: Radiofrequency ablation before intratumoral injection of (131)I-chTNT improves the tumor-to-normal tissue ratio in solid VX2 tumor.
abstract: OBJECTIVE
  This study was aimed to investigate whether the tumor necrosis induced by radiofrequency ablation (RFA) can improve the ratio of tumor-to-normal tissue (T/NT) after intratumoral injection of (131)I-chTNT.
  
  
  METHODS
  Eighteen New Zealand rabbits bearing VX2 tumor on the thigh were randomly divided into two treatment groups (control group: intratumoral injection of (131)I-chTNT alone; RFA group: RFA + intratumoral injection of (131)I-chTNT 3 days after RFA) and each group was further divided into three subgroups I, II, and III (1-2 cm, 2-3 cm, and 3-4 cm in maximum diameter, respectively), by the tumor size. SPECT was performed to evaluate the T/NT on days 1, 8, and 15 after (131)I-chTNT injection.
  
  
  RESULTS
  After treatment, all rabbits underwent the SPECT whole-body scan and the T/NT was analyzed. The results showed that T/NT in the RFA group (55.45±41.83) was significantly higher compared with the control group (7.23±5.61) (F=18.89, p=0.001). Meanwhile, a linear ascending trend was found for T/NT in the RFA group along with the follow-up time (r=0.47, p=0.01). The tumor size or the dose of (131)I-TNT injection had no significant effect on the variation of T/NT in both groups (p>0.05).
  
  
  CONCLUSIONS
  RFA before intratumoral injection of (131)I-chTNT can dramatically improve T/NT, demonstrating the potential application of this combination therapy.
authors: 1 Department of Medical Ultrasonics, The First Affiliated Hospital, Sun Yat-Sen University , Guangzhou, China .
authors: Shu-Guang
authors: SG
authors: Zheng
authors: Hui-Xiong
authors: HX
authors: Xu
authors: Ming-De
authors: MD
authors: Lu
authors: Dian-Chao
authors: DC
authors: Yue
authors: Xiao-Yan
authors: XY
authors: Xie
authors: Guang-Jian
authors: GJ
authors: Liu
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000008:administration & dosage*; D017115:Catheter Ablation / Q000379:methods*; D003131:Combined Modality Therapy; D015552:Injections, Intralesional; D007457:Iodine Radioisotopes / Q000008:administration & dosage*; D009374:Neoplasms, Experimental / Q000532:radiotherapy* / Q000601:surgery*; D011817:Rabbits; D019275:Radiopharmaceuticals / Q000008:administration & dosage*; D011897:Random Allocation; D015899:Tomography, Emission-Computed, Single-Photon
chemical_list: C576180:131I-chimeric TNT-1-B monoclonal antibody; D000911:Antibodies, Monoclonal; D007457:Iodine Radioisotopes; D019275:Radiopharmaceuticals
references: Oncogene. 2007 May 28;26(25):3734-44
references: 17530026
references: Mol Cancer Ther. 2011 Jun;10(6):1072-81
references: 21467164
references: Cancer Biol Ther. 2006 Mar;5(3):318-22
references: 16481737
references: Cancer Biother Radiopharm. 2009 Aug;24(4):417-26
references: 19694576
references: FASEB J. 2008 Mar;22(3):659-61
references: 17942826
references: AJNR Am J Neuroradiol. 2011 Apr;32(4):637-8
references: 21436340
references: Nucl Med Commun. 2011 Feb;32(2):147-54
references: 21116205
references: Cancer Immunol Immunother. 1991;33(3):158-64
references: 2044081
references: Cancer Biother Radiopharm. 2010 Dec;25(6):657-63
references: 21204759
references: Hepatology. 2011 Mar;53(3):1020-2
references: 21374666
references: J Cancer Res Ther. 2009 Sep;5 Suppl 1:S36-40
references: 20009292
references: Curr Radiopharm. 2011 Jul;4(3):248-60
references: 22201711
references: Lancet. 2012 Mar 31;379(9822):1245-55
references: 22353262
references: Cancer Biother Radiopharm. 2006 Jun;21(3):243-56
references: 16918301
references: Cancer Res. 1989 Aug 15;49(16):4578-85
references: 2743341
references: Clin Radiol. 2004 Jan;59(1):53-61
references: 14697375
references: Eur J Surg Oncol. 2011 Mar;37(3):258-64
references: 21208773
references: Expert Rev Anticancer Ther. 2006 Mar;6(3):445-61
references: 16503861
references: Cancer Treat Rev. 2012 Nov;38(7):854-60
references: 22226242
references: J Clin Oncol. 2005 Mar 1;23(7):1538-47
references: 15735129
references: Ann Surg. 2011 Feb;253(2):336-41
references: 21217519
references: Eur J Nucl Med Mol Imaging. 2011 Feb;38(2):271-3
references: 21161707
references: Semin Nucl Med. 2010 May;40(3):190-203
references: 20350628
references: ScientificWorldJournal. 2010 Oct 12;10:2070-89
references: 20953556
references: Cancer Biother Radiopharm. 2006 Feb;21(1):5-14
references: 16480326
references: Int J Radiat Oncol Biol Phys. 2006 Jun 1;65(2):435-44
references: 16690431
references: Cancer Control. 2012 Jul;19(3):196-203
references: 22710895
references: Cancer Metastasis Rev. 2005 Dec;24(4):539-67
references: 16408161
references: J Nucl Med. 2010 Nov;51(11):1780-7
references: 21051650
references: Cancer J. 2001 Mar-Apr;7(2):95-102
references: 11324771
references: Cancer. 2010 Feb 15;116(4 Suppl):1053-8
references: 20127950