title: Smoking and fluidity of erythrocyte membranes: a high resolution scanning electron and atomic force microscopy investigation.
abstract: Smoking affects the general health of an individual, however, the red blood cells (RBCs) and their architecture are particularly vulnerable to inhaled toxins related to smoking. Smoking is one of the lifestyle diseases that are responsible for the most deaths worldwide and an individual who smokes is exposed to excessive amounts of oxidants and toxins which generate up to 10(18) free radicals in the human body. Recently, it was reported that smoking decreases RBC membrane fluidity. Here we confirm this and we show changes visible in the topography of RBC membranes, using scanning electron microscopy (SEM). RBC membranes show bubble formation of the phospholipid layer, as well as balloon-like smooth areas; while their general discoid shapes are changed to form pointed extensions. We also investigate membrane roughness using atomic force microscopy (AFM) and these results confirm SEM results. Due to the vast capability of RBCs to be adaptable, their state of well-being is a major indication for the general health status of an individual. We conclude that these changes, using an old technique in a novel application, may provide new insights and new avenues for future improvements in clinical medicine pertaining to conditions like COPD.
authors: Department of Physiology, Faculty of Health Sciences, University of Pretoria, South Africa. Electronic address: resia.pretorius@up.ac.za.
authors: Etheresia
authors: E
authors: Pretorius
authors: Jeanette N
authors: JN
authors: du Plooy
authors: Prashilla
authors: P
authors: Soma
authors: Ina
authors: I
authors: Keyser
authors: Antoinette V
authors: AV
authors: Buys
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D004910:Erythrocyte Membrane / Q000473:pathology* / Q000502:physiology*; D005260:Female; D006801:Humans; D008297:Male; D008560:Membrane Fluidity / Q000502:physiology; D018625:Microscopy, Atomic Force; D008855:Microscopy, Electron, Scanning; D008875:Middle Aged; D012907:Smoking / Q000097:blood* / Q000453:epidemiology; D013481:Superoxides / Q000097:blood; D055815:Young Adult
chemical_list: D013481:Superoxides
keywords: Electron microscopy; Erythrocytes; Membranes; Smoking