title: Neurochemistry of myenteric plexus neurons of bank vole (Myodes glareolus) ileum.
abstract: The neurochemistry of enteric neurons differs among species of small laboratory rodents (guinea-pig, mouse, rat). In this study we characterized the phenotype of ileal myenteric plexus (MP) neuronal cells and fibers of the bank vole (Myodes glareolus), a common rodent living in Europe and in Northern Asia which is also employed in prion experimental transmission studies. Six neuronal markers were tested: choline acetyltransferase (ChAT), neuronal nitric oxide synthase (nNOS), calbindin (CALB), calcitonin gene-related peptide (CGRP) and substance P (SP), along with HuC/D as a pan-neuronal marker. Neurons expressing ChAT- and nNOS-immunoreactivity (IR) were 36 ± 12% and 24 ± 5%, respectively. Those expressing CGRP-, SP- and CALB-IR were 3 ± 3%, 21 ± 5% and 6 ± 2%, respectively. Therefore, bank vole MPs differ consistently from murine MPs in neurons expressing CGRP-, SP- and CALB-IR. These data may contribute to define the prion susceptibility of neuron cell populations residing within ileal MPs from bank voles, along with their morpho-functional alterations following oral experimental prion challenge.
authors: Department of Veterinary Medical Science, University of Bologna, Ozzano dell'Emilia, Bologna, Italy; Department of Basic Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
authors: J
authors: J
authors: Sadeghinezhad
authors: C
authors: C
authors: Sorteni
authors: G
authors: G
authors: Di Guardo
authors: C
authors: C
authors: D'Agostino
authors: U
authors: U
authors: Agrimi
authors: R
authors: R
authors: Nonno
authors: R
authors: R
authors: Chiocchetti
mesh_terms: D000818:Animals; D003411:Arvicolinae / Q000378:metabolism* / Q000502:physiology; D064026:Calbindins / Q000378:metabolism; D015740:Calcitonin Gene-Related Peptide / Q000378:metabolism; D002795:Choline O-Acetyltransferase / Q000378:metabolism; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect / Q000662:veterinary; D008297:Male; D008856:Microscopy, Fluorescence / Q000662:veterinary; D009197:Myenteric Plexus / Q000378:metabolism* / Q000502:physiology; D052248:Nitric Oxide Synthase Type I / Q000378:metabolism; D013373:Substance P / Q000378:metabolism
chemical_list: D064026:Calbindins; D013373:Substance P; D052248:Nitric Oxide Synthase Type I; D002795:Choline O-Acetyltransferase; D015740:Calcitonin Gene-Related Peptide
keywords: Bank vole; Enteric nervous system; Immunohistochemistry; Neurochemical code