title: Emodin elicits cytotoxicity in human lung adenocarcinoma A549 cells through inducing apoptosis.
abstract: This study investigated the mechanism of the cytotoxic effect of emodin, an active anthraquinone, on human lung adenocarcinoma A549 cells. In vitro growth inhibition and suppression on colony forming were used to evaluate the effects of emodin on A549 cells. Emodin's ability in changing the expressions of apoptosis-related genes was studied by real-time RT-PCR. Emodin could significantly inhibit the growth of A549 cells with IC50 = 16.85 μg/ml (~60 μM). It also concentration dependently inhibited the colony-forming ability of A549 cells with IC50 = 7.60 μg/ml (~30 μM). Hallmarks of apoptosis, such as single-strand DNA breakage and DNA fragmentation, were observed in A549 cells treated with emodin. Emodin (72 h) treatment could up-regulate the gene expression of FASL (p < 0.05) and down-regulate the gene expression of C-MYC (p < 0.01), but induce no significant changes in the gene expressions of MCL1, GAPDH, BAX and CCND1. These results suggest that emodin could induce growth inhibition and apoptosis in A549 cells through modifying the extrinsic apoptotic pathways and the induction of cell cycle arrest.
authors: Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hong Kong, China.
authors: Wing-Yan
authors: WY
authors: Li
authors: Yam-Fung
authors: YF
authors: Ng
authors: Huan
authors: H
authors: Zhang
authors: Zi-Dong
authors: ZD
authors: Guo
authors: De-Jian
authors: DJ
authors: Guo
authors: Yiu-Wa
authors: YW
authors: Kwan
authors: George Pak-Heng
authors: GP
authors: Leung
authors: Simon Ming-Yuen
authors: SM
authors: Lee
authors: Peter Hoi-Fu
authors: PH
authors: Yu
authors: Shun-Wan
authors: SW
authors: Chan
mesh_terms: D000230:Adenocarcinoma / Q000188:drug therapy* / Q000235:genetics; D000077192:Adenocarcinoma of Lung; D017209:Apoptosis / Q000187:drug effects* / Q000235:genetics; D051017:Apoptosis Regulatory Proteins / Q000235:genetics; D059447:Cell Cycle Checkpoints / Q000187:drug effects / Q000235:genetics; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D053904:DNA Breaks, Single-Stranded / Q000187:drug effects; D053938:DNA Fragmentation / Q000187:drug effects; D004642:Emodin / Q000494:pharmacology*; D015870:Gene Expression / Q000187:drug effects / Q000235:genetics; D006801:Humans; D008175:Lung Neoplasms / Q000188:drug therapy* / Q000235:genetics
chemical_list: D051017:Apoptosis Regulatory Proteins; D004642:Emodin
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