title: Generation and analysis of biosensors to measure mechanical forces within cells.
abstract: The inability to measure mechanical forces within cells has been limiting our understanding of how mechanical information is processed on the molecular level. In this chapter, we describe a method that allows the analysis of force propagation across distinct proteins within living cells using Förster resonance energy transfer (FRET)-based biosensors.
authors: Group of Molecular Mechanotransduction, Max-Planck-Institute of Biochemistry, Martinsried, Germany.
authors: Katharina
authors: K
authors: Austen
authors: Carleen
authors: C
authors: Kluger
authors: Andrea
authors: A
authors: Freikamp
authors: Anna
authors: A
authors: Chrostek-Grashoff
authors: Carsten
authors: C
authors: Grashoff
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D002478:Cells, Cultured; D018076:DNA, Complementary / Q000235:genetics; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D006801:Humans; D040542:Mechanotransduction, Cellular; D009841:Oligonucleotides / Q000235:genetics; D013314:Stress, Mechanical; D014162:Transfection / Q000379:methods
chemical_list: D018076:DNA, Complementary; D009841:Oligonucleotides