title: Electroacupuncture Could Regulate the NF-κB Signaling Pathway to Ameliorate the Inflammatory Injury in Focal Cerebral Ischemia/Reperfusion Model Rats.
abstract: The activated nuclear factor-KappaB signaling pathway plays a critical role in inducing inflammatory injury. It has been reported that electroacupuncture could be an effective anti-inflammatory treatment. We aimed to explore the complex mechanism by which EA inhibits the activation of the NF- κ B signal pathway and ameliorate inflammatory injury in the short term; the effects of NEMO Binding Domain peptide for this purpose were compared. Focal cerebral I/R was induced by middle cerebral artery occlusion for 2 hrs. Total 380 male Sprague-Dawley rats are in the study. The neurobehavioral scores, infarction volumes, and the levels of IL-1 β and IL-13 were detected. NF- κ B p65, I κ B α , IKK α , and IKK β were analyzed and the ability of NF- κ B binding DNA was investigated. The EA treatment and the NBD peptide treatment both reduced infarct size, improved neurological scores, and regulated the levels of IL-1 β and IL-13. The treatment reduced the expression of IKK α and IKK β and altered the expression of NF- κ B p65 and I κ B α in the cytoplasm and nucleus; the activity of NF- κ B was effectively reduced. We conclude that EA treatment might interfere with the process of NF- κ B nuclear translocation. And it also could suppress the activity of NF- κ B signaling pathway to ameliorate the inflammatory injury after focal cerebral ischemia/reperfusion.
authors: Department of Neurology, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China ; Chongqing Key Laboratory of Neurology, 1 Youyi Road, Yuzhong District, Chongqing 400016, China.
authors: Wen-Yi
authors: WY
authors: Qin
authors: Yong
authors: Y
authors: Luo
authors: Ling
authors: L
authors: Chen
authors: Tao
authors: T
authors: Tao
authors: Yang
authors: Y
authors: Li
authors: Yan-Li
authors: YL
authors: Cai
authors: Ya-Hui
authors: YH
authors: Li
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