title: Novel immunogenic baculovirus expressed virus-like particles of foot-and-mouth disease (FMD) virus protect guinea pigs against challenge.
abstract: Vaccination is a well accepted strategy for control of foot-and-mouth disease (FMD) in endemic countries. Currently, chemically inactivated virus antigens are used for preparation of FMD vaccine. To develop a non-infectious and safe recombinant vaccine, we expressed structural polypeptide of FMDV (O/IND/R2/75) using baculovirus expression system. We show that inclusion of mutated viral 3C protease in frame with the polypeptide (P1-2A), enhanced the yield of structural proteins. The structural proteins retained antigenicity and assembled into empty virus-like particles (VLPs). Immunization of guinea pigs with purified fractions of the VLPs resulted in humoral and cell mediated immune response by 4 weeks. The VLPs elicited comparable humoral immune response and relatively higher cell mediated immune response, when compared to conventional vaccine in guinea pigs. Further, up to 70% of the VLP immunized guinea pigs were protected against challenge with homologous guinea pig adapted virus. Our results highlight the application of recombinant FMDV VLPs in FMD vaccination.
authors: Foot-and-Mouth Disease Vaccine Centre, Indian Veterinary Research Institute, Hebbal, Bangalore 560 024, Karnataka, India.
authors: S A
authors: SA
authors: Bhat
authors: P
authors: P
authors: Saravanan
authors: M
authors: M
authors: Hosamani
authors: S H
authors: SH
authors: Basagoudanavar
authors: B P
authors: BP
authors: Sreenivasa
authors: R P
authors: RP
authors: Tamilselvan
authors: R
authors: R
authors: Venkataramanan
mesh_terms: D000818:Animals; D057134:Antibodies, Neutralizing / Q000276:immunology; D000956:Antigens, Viral / Q000276:immunology; D016367:Baculoviridae / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005260:Female; D005536:Foot-and-Mouth Disease / Q000276:immunology / Q000517:prevention & control*; D029544:Foot-and-Mouth Disease Virus / Q000276:immunology*; D006168:Guinea Pigs; D007111:Immunity, Cellular / Q000276:immunology; D056724:Immunity, Humoral / Q000276:immunology; D008297:Male; D009500:Neutralization Tests / Q000662:veterinary; D014614:Vaccines, Synthetic / Q000276:immunology / Q000627:therapeutic use; D058425:Vaccines, Virus-Like Particle / Q000627:therapeutic use*; D014765:Viral Vaccines / Q000276:immunology / Q000627:therapeutic use*
chemical_list: D057134:Antibodies, Neutralizing; D000956:Antigens, Viral; D014614:Vaccines, Synthetic; D058425:Vaccines, Virus-Like Particle; D014765:Viral Vaccines
keywords: Baculovirus expression system; Foot-and-mouth disease virus; Protection against challenge; Virus like particles; Virus neutralizing antibody