title: Expression pattern of STAT5A gene during early bovine embryogenesis.
abstract: Growth hormone (GH) plays an important role in early embryo development. It has been shown to activate multiple pathways, the most comprehensively studied being the STAT/JAK (Signal transducers and activators of transcription/Janus kinase) pathway. The objective of the present study was to investigate STAT5A gene expression during early bovine embryogenesis. Real-time polymerase chain reaction (RT-PCR) was used to measure the abundance of STAT5A transcripts. The mRNA was present at all stages of preimplantation bovine embryos investigated. The most abundant STAT5A expression occurred at the 2-cell stage. Expression was markedly reduced between the 4-cell and 8-cell stages, coinciding with the known time of embryo genome activation and loss of maternal mRNAs. This finding suggests that the embryonic STAT5A gene is primarily activated by maternal gene products.
authors: Technische Universität München,Liesel-Beckmann-Str. 1,85354 Freising,Germany.
authors: Krzysztof
authors: K
authors: Flisikowski
authors: Ludwig-Maximilians-Universität München,Hackerstrasse 27,85764 Oberschleissheim,Germany.
authors: Marc
authors: M
authors: Boulhaeve
authors: Ludwig-Maximilians-Universität München,Hackerstrasse 27,85764 Oberschleissheim,Germany.
authors: Fabiola P
authors: FP
authors: Lopes
authors: Ludwig-Maximilians-Universität München,Hackerstrasse 27,85764 Oberschleissheim,Germany.
authors: Eckhard
authors: E
authors: Wolf
authors: Institute of Genetics and Animal Breeding,Polish Academy of Sciences,Jastrzebiec,05-552 Magdalenka,Poland.
authors: Lech
authors: L
authors: Zwierzchowski
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000166:cytology / Q000502:physiology*; D002417:Cattle / Q000196:embryology* / Q000235:genetics; D005260:Female; D005307:Fertilization in Vitro; D018507:Gene Expression Regulation, Developmental; D060888:Real-Time Polymerase Chain Reaction; D050799:STAT5 Transcription Factor / Q000235:genetics*
chemical_list: D050799:STAT5 Transcription Factor