title: RT-isoPCR: nested, high multiplex mRNA amplification.
abstract: RT-isoPCR provides multiplex amplification of mRNA targets using a first-stage multiplex RT-PCR reaction with subsequent isothermal amplification for individual target loci detection. We demonstrate detection of 24 mRNA targets with high specificity and sensitivity without compromising sample variation or introducing biases between targets.
authors: Atonomics, Vestre Teglgade 10, DK-2450 Copenhagen SV, Denmark. martin.j.soe@gmail.com pw@atonomics.com.
authors: Martin Jensen
authors: MJ
authors: Søe
authors: Peter
authors: P
authors: Warthoe
mesh_terms: D006801:Humans; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D012333:RNA, Messenger / Q000032:analysis* / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*
chemical_list: D012333:RNA, Messenger