title: Micropatterning cell adhesion on polyacrylamide hydrogels.
abstract: Cell shape and substrate rigidity play critical roles in regulating cell behaviors and fate. Controlling cell shape on elastic adhesive materials holds great promise for creating a physiologically relevant culture environment for basic and translational research and clinical applications. However, it has been technically challenging to create high-quality adhesive patterns on compliant substrates. We have developed an efficient and economical method to create precise micron-scaled adhesive patterns on the surface of a hydrogel (Rape et al., Biomaterials 32:2043-2051, 2011). This method will facilitate the research on traction force generation, cellular mechanotransduction, and tissue engineering, where precise controls of both materials rigidity and adhesive patterns are important.
authors: Department of Biomedical Engineering, Carnegie Mellon University, Pittsburgh, PA, USA.
authors: Jian
authors: J
authors: Zhang
authors: Wei-Hui
authors: WH
authors: Guo
authors: Andrew
authors: A
authors: Rape
authors: Yu-Li
authors: YL
authors: Wang
mesh_terms: D016475:3T3 Cells; D000180:Acrylic Resins / Q000378:metabolism*; D000818:Animals; D002448:Cell Adhesion / Q000502:physiology*; D002450:Cell Communication / Q000502:physiology*; D002460:Cell Line; D002465:Cell Movement / Q000502:physiology; D020100:Hydrogels / Q000378:metabolism*; D040542:Mechanotransduction, Cellular / Q000502:physiology; D051379:Mice; D013314:Stress, Mechanical
chemical_list: D000180:Acrylic Resins; D020100:Hydrogels; C016680:polyacrylamide gels