title: Lonicera caerulea fruits reduce UVA-induced damage in hairless mice.
abstract: UVA photons are less energetic than UVB photons but they are more abundant in solar radiation. Modern tools have shown that UVA light has serious adverse effects on the skin. We investigated the effect of consuming Lonicera caerulea berries on UVA-induced damage in SKH-1 mice. The mice were fed a diet containing L. caerulea berries (10%, w/w) for 14 days before a single UVA (30 J/cm(2)) treatment. Effects on haematological and antioxidant parameters were evaluated 4 and 24h after irradiation. The bioavailability of L. caerulea phenolics was also assessed. Consuming the L. caerulea berry-enriched diet caused reduced malondialdehyde production and increased catalase activity and glutathione levels were found in skin and erythrocytes. UVA-induced NADPH:quinone oxidoreductase-1 and gamma-L-glutamate-L-cysteine ligase protein in skin were reduced in mice fed L. caerulea berries. Enhanced heme oxygenase-1 level in skin, interleukin-17 in plasma and reduced interleukin-12 levels in plasma were found in the mice on the experimental diet. Histological (pyknotic) changes in the nuclei of basal cells induced by UVA exposure were reduced in L. caerulea berry consuming animals. HLPC-MS analysis showed high concentrations of hippuric acid, one of the main metabolites of aromatic amino acids and phenolic compounds, in skin, liver, urine and faeces of mice consuming the berries. Taken together, consumption of L. caerulea berries affords protection from the adverse effects of a single UVA exposure mainly via modulation of antioxidant parameters.
authors: Department of Medical Chemistry and Biochemistry, Faculty of Medicine and Dentistry, Palacky University, Hněvotínská 3, 775 15 Olomouc, Czech Republic.
authors: Jitka
authors: J
authors: Vostálová
authors: Adéla
authors: A
authors: Galandáková
authors: Irena
authors: I
authors: Palíková
authors: Jitka
authors: J
authors: Ulrichová
authors: Dalibor
authors: D
authors: Doležal
authors: Radka
authors: R
authors: Lichnovská
authors: Jana
authors: J
authors: Vrbková
authors: Alena
authors: A
authors: Rajnochová Svobodová
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D004032:Diet; D004798:Enzymes / Q000378:metabolism; D004912:Erythrocytes / Q000378:metabolism / Q000528:radiation effects; D005260:Female; D005638:Fruit / Q000737:chemistry / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D006626:Hippurates / Q000032:analysis / Q000652:urine; D018664:Interleukin-12 / Q000097:blood; D020381:Interleukin-17 / Q000097:blood; D008099:Liver / Q000737:chemistry; D031239:Lonicera / Q000737:chemistry* / Q000378:metabolism; D008315:Malondialdehyde / Q000378:metabolism; D051379:Mice; D008812:Mice, Hairless; D012867:Skin / Q000378:metabolism / Q000473:pathology / Q000528:radiation effects*; D014466:Ultraviolet Rays
chemical_list: D000975:Antioxidants; D004798:Enzymes; D006626:Hippurates; D020381:Interleukin-17; D018664:Interleukin-12; D008315:Malondialdehyde; D005978:Glutathione; C030514:hippuric acid
keywords: Blue honeysuckle; Erythrocyte; Hairless mice; Liver; Skin; UVA radiation