title: Breed-linked polymorphisms of porcine toll-like receptor 2 (TLR2) and TLR4 and the primary investigation on their relationship with prevention against Mycoplasma pneumoniae and bacterial LPS challenge.
abstract: Toll-like receptors (TLRs) play a crucial role in innate immunity, serving as pattern-recognition receptors and the first barrier in host defense against microbial infections. Genetic variations of TLR2 and TLR4 are closely associated with a variety of infectious diseases, particularly lung diseases. In this study, we detected six and four single nucleotide polymorphisms (SNPs) in the coding sequences of porcine TLR2 and TLR4 genes, respectively. Only SNP 1027C>A of TLR4 was shown to be markedly biased in Western and Oriental pig populations. Hence, the susceptibility of pigs with different genotype at position 1027C>A to Mycoplasma hyopneumoniae (Mhp) infection was investigated, and changes to the expression of TLR2, TLR4, TNF-α and IL-1β were monitored. The results showed that there was no significant difference in susceptibility to Mhp infection between AA and CC individuals despite expression levels for all detected genes of the challenge groups being significantly higher than the corresponding control groups. Furthermore, porcine alveolar macrophages of different genotype were collected and stimulated by lipopolysaccharide. We found that the expression of TLR2, TLR4, TNF-α and IL-1β genes were enhanced to different levels by lipopolysaccharide stimulation. TLR2 and TLR4 gene expressions and their rates of increase of 1027CC pigs were significantly higher than for 1027AC pigs (P < 0.01), while TNF-α and IL-1β expressions were significantly lower than for 1027AC pigs (P < 0.01). We predict that allele C at position 1027 of the TLR4 gene contributes to the pig's immune response to gram-negative bacterial infections.
authors: Institute of Animal Science, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, People's Republic of China.
authors: Xiaomin
authors: X
authors: Fang
authors: Xiao
authors: X
authors: Liu
authors: Cui
authors: C
authors: Meng
authors: Yanfeng
authors: Y
authors: Fu
authors: Xuemin
authors: X
authors: Wang
authors: Bixia
authors: B
authors: Li
authors: Feng
authors: F
authors: Tu
authors: Fang
authors: F
authors: Zhao
authors: Shouwen
authors: S
authors: Ren
mesh_terms: D000818:Animals; D001947:Breeding; D002478:Cells, Cultured; D005260:Female; D007113:Immunity, Innate / Q000187:drug effects; D008070:Lipopolysaccharides / Q000494:pharmacology*; D016676:Macrophages, Alveolar / Q000166:cytology / Q000187:drug effects / Q000276:immunology; D009177:Mycoplasma pneumoniae / Q000235:genetics / Q000276:immunology / Q000472:pathogenicity*; D011019:Pneumonia, Mycoplasma / Q000235:genetics / Q000276:immunology / Q000517:prevention & control*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D013552:Swine; D051195:Toll-Like Receptor 2 / Q000235:genetics* / Q000276:immunology; D051197:Toll-Like Receptor 4 / Q000235:genetics* / Q000276:immunology
chemical_list: D008070:Lipopolysaccharides; D051195:Toll-Like Receptor 2; D051197:Toll-Like Receptor 4
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