title: Effects of cellular activation on anti-HIV-1 restriction factor expression profile in primary cells.
abstract: Expression of cell-intrinsic antiviral factors suppresses HIV-1 replication. We hypothesized that cellular activation modulates host restriction and susceptibility to HIV-1 infection. We measured the gene expression of 34 antiviral factors in healthy peripheral blood mononuclear cells (PBMC). Cellular activation induced expression of interferon-stimulated gene 15 (ISG15), tripartite motif 5α (TRIM5α), bone marrow stromal cell antigen 2 (BST-2)/tetherin, and certain apolipoprotein B mRNA editing enzyme 3 (APOBEC3) family members. Expression of RTF1, RNA polymerase II-associated factor 1 (PAF1), TRIM11, TRIM26, and BST-2/tetherin correlated with decreased HIV-1 infectivity. This report demonstrates synchronous effects of activation-induced antiviral genes on HIV-1 infectivity, providing candidates for pharmacological manipulation.
authors: Division of Experimental Medicine, University of California San Francisco, San Francisco, California, USA.
authors: Rui André Saraiva
authors: RA
authors: Raposo
authors: Mohamed
authors: M
authors: Abdel-Mohsen
authors: Miroslawa
authors: M
authors: Bilska
authors: David C
authors: DC
authors: Montefiori
authors: Douglas F
authors: DF
authors: Nixon
authors: Satish K
authors: SK
authors: Pillai
mesh_terms: D002478:Cells, Cultured; D020869:Gene Expression Profiling; D015497:HIV-1 / Q000276:immunology*; D006801:Humans; D007113:Immunity, Innate; D007963:Leukocytes, Mononuclear / Q000276:immunology* / Q000821:virology*
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