title: Nontraditional localization and retention signals localize human cytomegalovirus pUL34 to the nucleus.
abstract: The human cytomegalovirus (HCMV) UL34 proteins localize to the nucleus. We identified a 204-amino-acid region, amino acids 40 to 244, as required for nuclear retention. A highly conserved 12-amino-acid section, amino acids 198 to 210, was required for nuclear localization. Although the nuclear localization and retention signals of pUL34 overlap the domain required for DNA-binding activity, the two regions are separable by point mutations. Our results presented here identify the first example of an HCMV protein with separable nuclear localization and retention signals.
authors: Department of Biomedical Sciences and Program in Molecular and Cellular Biology, Heritage College of Osteopathic Medicine, Ohio University, Athens, Ohio, USA.
authors: Bonita J
authors: BJ
authors: Biegalke
mesh_terms: D002467:Cell Nucleus / Q000737:chemistry* / Q000821:virology; D003587:Cytomegalovirus / Q000235:genetics / Q000502:physiology*; D004252:DNA Mutational Analysis; D006801:Humans; D019913:Nuclear Localization Signals; D017354:Point Mutation; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D019913:Nuclear Localization Signals; D014764:Viral Proteins
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