title: Targeted heritable mutation and gene conversion by Cas9-CRISPR in Caenorhabditis elegans.
abstract: We have achieved targeted heritable genome modification in Caenorhabditis elegans by injecting mRNA of the nuclease Cas9 and Cas9 guide RNAs. This system rapidly creates precise genomic changes, including knockouts and transgene-instructed gene conversion.
authors: Friedrich Miescher Institute for Biomedical Research, CH-4058 Basel, Switzerland.
authors: Iskra
authors: I
authors: Katic
authors: Helge
authors: H
authors: Großhans
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D001483:Base Sequence; D064113:CRISPR-Cas Systems; D017173:Caenorhabditis elegans / Q000235:genetics*; D029742:Caenorhabditis elegans Proteins / Q000235:genetics; D015220:Calcium Channels / Q000235:genetics; D053903:DNA Breaks, Double-Stranded; D005785:Gene Conversion; D055786:Gene Knockout Techniques; D018390:Gene Targeting; D017238:Genes, Helminth; D018095:Germ-Line Mutation; D017393:RNA Editing / Q000235:genetics; D018510:RNA, Helminth / Q000235:genetics
chemical_list: D029742:Caenorhabditis elegans Proteins; D015220:Calcium Channels; D018510:RNA, Helminth; C585116:UNC-36 protein, C elegans
keywords: CRISPR; Caenorhabditis elegans; Cas9; gene conversion; genome editing; homologous recombination
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