title: Increased cytokine/chemokines in serum from asthmatic and non-asthmatic patients with viral respiratory infection.
abstract: BACKGROUND
  Respiratory viral infections can induce different cytokine/chemokine profiles in lung tissues and have a significant influence on patients with asthma. There is little information about the systemic cytokine status in viral respiratory-infected asthmatic patients compared with non-asthmatic patients.
  
  
  OBJECTIVE
  The aim of this study was to determine changes in circulating cytokines (IL-1β, TNF-α, IL-4, IL-5) and chemokines (MCP1: monocyte chemoattractant protein-1 and RANTES: regulated on activation normal T cell expressed and secreted) in patients with an asthmatic versus a non-asthmatic background with respiratory syncytial virus, parainfluenza virus or adenovirus respiratory infection. In addition, human monocyte cultures were incubated with respiratory viruses to determine the cytokine/chemokine profiles.
  
  
  METHODS
  Patients with asthmatic (n = 34) and non-asthmatic (n = 18) history and respiratory infections with respiratory syncytial virus, parainfluenza, and adenovirus were studied. Healthy individuals with similar age and sex (n = 10) were used as controls. Cytokine/chemokine content in blood and culture supernatants was determined by ELISA. Monocytes were isolated by Hystopaque gradient and cocultured with each of the above-mentioned viruses.
  
  
  RESULTS
  Similar increased cytokine concentrations were observed in asthmatic and non-asthmatic patients. However, higher concentrations of chemokines were observed in asthmatic patients. Virus-infected monocyte cultures showed similar cytokine/chemokine profiles to those observed in the patients.
  
  
  CONCLUSIONS
  Circulating cytokine profiles induced by acute viral lung infection were not related to asthmatic status, except for chemokines that were already increased in the asthmatic status. Monocytes could play an important role in the increased circulating concentration of cytokines found during respiratory viral infections.
authors: Facultad de Medicina, Instituto de Investigaciones Clínicas "Dr. Américo Negrette", Universidad del Zulia, Maracaibo, Venezuela.
authors: María J
authors: MJ
authors: Giuffrida
authors: Nereida
authors: N
authors: Valero
authors: Jesús
authors: J
authors: Mosquera
authors: Melchor
authors: M
authors: Alvarez de Mon
authors: Betulio
authors: B
authors: Chacín
authors: Luz Marina
authors: LM
authors: Espina
authors: Jennifer
authors: J
authors: Gotera
authors: John
authors: J
authors: Bermudez
authors: Alibeth
authors: A
authors: Mavarez
mesh_terms: D000256:Adenoviridae / Q000276:immunology; D000257:Adenoviridae Infections / Q000276:immunology* / Q000473:pathology; D000293:Adolescent; D000328:Adult; D000368:Aged; D001249:Asthma / Q000150:complications; D002478:Cells, Cultured; D002648:Child; D016207:Cytokines / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000276:immunology; D018184:Paramyxoviridae Infections / Q000276:immunology* / Q000473:pathology; D018357:Respiratory Syncytial Virus Infections / Q000276:immunology* / Q000473:pathology; D012136:Respiratory Syncytial Viruses / Q000276:immunology; D012141:Respiratory Tract Infections / Q000276:immunology* / Q000473:pathology; D018108:Respirovirus / Q000276:immunology; D044967:Serum / Q000737:chemistry; D055815:Young Adult
chemical_list: D016207:Cytokines
keywords: Asthma; chemokines; interleukins; viral lung infection
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