title: TNFR2 Expression on CD25(hi)FOXP3(+) T Cells Induced upon TCR Stimulation of CD4 T Cells Identifies Maximal Cytokine-Producing Effectors.
abstract: In this study, we show that CD25(hi)TNFR2(+) cells can be rapidly generated in vitro from circulating CD4 lymphocytes by polyclonal stimuli anti-CD3 in the presence of anti-CD28. The in vitro induced CD25(hi)TNFR2(+) T cells express a conventional regulatory T cells phenotype FOXP3(+)CTLA4(+)CD127(lo/-), but produce effector and immunoregulatory cytokines including IL-2, IL-10, and IFN-g. These induced CD25(hi)TNFR2(+) T cells do not suppress target cell proliferation, but enhance it instead. Thus the CD25(hi)TNFR2(+) phenotype induced rapidly following CD3/28 cross linking of CD4 T cells identifies cells with maximal proliferative and effector cytokine-producing capability. The in vivo counterpart of this cell population may play an important role in immune response initiation.
authors: Department of Immunology, Monash University , Clayton, VIC , Australia.
authors: Chindu
authors: C
authors: Govindaraj
authors: Karen
authors: K
authors: Scalzo-Inguanti
authors: Anja
authors: A
authors: Scholzen
authors: Shuo
authors: S
authors: Li
authors: Magdalena
authors: M
authors: Plebanski
keywords: FOXP3; TNFR2; Th1; effector cells; regulatory T cells
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