title: Electrophilic and reactive oxygen species detoxification potentials of chalcone dimers is mediated by redox transcription factor Nrf-2.
abstract: Nuclear erythroid related factor-2 (Nrf2), a redox-transcription factor, plays a critical role in the detoxification of electrophilic and reactive oxygen species that halt various biochemical and molecular processes. This makes it a candidate for regulation by polyphenols. This study investigates the capability of chalcone dimers (lophirones B and C) to induce expressions and activities of cytoprotective enzymes. Chalcone dimers administration to rats not only induced the Nrf2, but also suppressed cytoplasmic Kelch-like ECH-associated protein 1 (Keap1) expressions. In addition, the chalcone dimers significantly (p < 0.05) increased the expressions and activities of nicotinamide adenine dinucleotide and reduced quinone oxidoreductase-1, glutathione-S-transferase, epoxide hydrolase and uridyl glucuronosyl transferase in rat liver. Also, activities of superoxide dismutase, catalase, glutathione peroxidase, and glutathione reductase in rat liver increased significantly (p < 0.05). Overall, lophirones B and C increased the expressions and activities of cytoprotective proteins in rat liver possibly through the reduction of cytoplasmic Keap1 expression, leading to the nuclear translocation of Nrf2.
authors: Antioxidants, Free Radicals, Functional Foods and Toxicology Research Laboratory, Department of Biological Sciences, Al-Hikmah University, Ilorin, Nigeria. ajiboyeyong@yahoo.com.
authors: Taofeek O
authors: TO
authors: Ajiboye
authors: Musa T
authors: MT
authors: Yakubu
authors: Adenike T
authors: AT
authors: Oladiji
mesh_terms: D000818:Animals; D015153:Blotting, Western; D047188:Chalcones / Q000737:chemistry / Q000378:metabolism*; D019281:Dimerization; D008658:Inactivation, Metabolic; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D051267:NF-E2-Related Factor 2 / Q000502:physiology*; D010084:Oxidation-Reduction; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000378:metabolism*
chemical_list: D047188:Chalcones; D051267:NF-E2-Related Factor 2; C495637:Nfe2l2 protein, rat; D017382:Reactive Oxygen Species; C000613963:lophirone B; C000613964:lophirone C
keywords: Chalcone Dimers; Free Radicals and Electrophile Detoxifying Enzymes; Keap-1; Lophirone B; Lophirone C; Nrf2; Reactive Oxygen Species