title: Characterization and modulation of glucose uptake in a human blood-brain barrier model.
abstract: The blood-brain barrier (BBB) plays a key role in limiting and regulating glucose access to glial and neuronal cells. In this work glucose uptake on a human BBB cell model (the hCMEC/D3 cell line) was characterized. The influence of some hormones and diet components on glucose uptake was also studied. ³H-2-deoxy-D-glucose ([³H]-DG) uptake for hCMEC/D3 cells was evaluated in the presence or absence of tested compounds. [³H]-DG uptake was sodium- and energy-independent. [³H]-DG uptake was regulated by Ca²⁺ and calmodulin but not by MAPK kinase pathways. PKC, PKA and protein tyrosine kinase also seem to be involved in glucose uptake modulation. Progesterone and estrone were found to decrease ³H-DG uptake. Catechin and epicatechin did not have any effect, but their methylated metabolites increased [³H]-DG uptake. Quercetin and myricetin decreased [³H]-DG uptake, and glucuronic acid-conjugated quercetin did not have any effect. These cells expressed GLUT1, GLUT3 and SGLT1 mRNA.
authors: Department of Biochemistry (U38.FCT), Faculty of Medicine, University of Porto, Al. Prof. Hernâni Monteiro, 4200-319, Porto, Portugal.
authors: Manuela
authors: M
authors: Meireles
authors: Fátima
authors: F
authors: Martel
authors: João
authors: J
authors: Araújo
authors: Celestino
authors: C
authors: Santos-Buelga
authors: Susana
authors: S
authors: Gonzalez-Manzano
authors: Montserrat
authors: M
authors: Dueñas
authors: Victor
authors: V
authors: de Freitas
authors: Nuno
authors: N
authors: Mateus
authors: Conceição
authors: C
authors: Calhau
authors: Ana
authors: A
authors: Faria
mesh_terms: D000305:Adrenal Cortex Hormones / Q000502:physiology; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D001692:Biological Transport; D001812:Blood-Brain Barrier / Q000378:metabolism*; D002392:Catechin / Q000502:physiology; D002460:Cell Line; D003847:Deoxyglucose / Q000378:metabolism*; D015870:Gene Expression; D051246:Glucose Transport Proteins, Facilitative / Q000235:genetics / Q000378:metabolism; D042341:Gonadal Hormones / Q000502:physiology; D006801:Humans; D019751:Intramolecular Transferases / Q000235:genetics / Q000378:metabolism; D008954:Models, Biological; D015398:Signal Transduction; D012964:Sodium / Q000378:metabolism
chemical_list: D000305:Adrenal Cortex Hormones; D029681:Arabidopsis Proteins; D051246:Glucose Transport Proteins, Facilitative; D042341:Gonadal Hormones; D002392:Catechin; D003847:Deoxyglucose; D012964:Sodium; D019751:Intramolecular Transferases; C568454:marneral synthase, Arabidopsis
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