title: Molecular identification and phylogenetic analysis of Heterakis gallinae from native chickens in Egypt.
abstract: The genomic DNA was extracted from nematode parasites, Heterakis gallinae from specific host, native chickens. The polymerase chain reaction (PCR) was applied to make amplification of the DNA content that aids in the further studies. The primer was used in the PCR reactions that have the following sequences: forward (5'-GTTTCCGTAGGTGAACCTGC-3') and reverse (5'-ATATGCTTAAGTTCAGCGGGT-3'). It gave total amplified fragment markers at 914 bp. The gene sequences obtained from Egyptian H. gallinae was to make phylogenetic relationships of Egyptian H. gallinae with other nematodes on the database of an accession number (AJ876757), showing that it was nearly similar to Heterakis gallinarum.
authors: Pathology and Parasitology Department, Faculty of Veterinary Medicine, Damanhour University, Damanhour, Egypt, ebazh@yahoo.com.
authors: Eman K A
authors: EK
authors: Bazh
mesh_terms: D000818:Animals; D002645:Chickens; D018509:DNA, Helminth / Q000235:genetics; D004534:Egypt / Q000453:epidemiology; D009348:Nematoda / Q000145:classification / Q000235:genetics*; D009349:Nematode Infections / Q000453:epidemiology / Q000469:parasitology / Q000662:veterinary*; D010802:Phylogeny; D011201:Poultry Diseases / Q000453:epidemiology / Q000469:parasitology*
chemical_list: D018509:DNA, Helminth
references: Acta Trop. 1997 Nov;68(2):191-200
references: 9386794
references: Parasitol Res. 2013 Sep;112(9):3223-7
references: 23793336
references: Parasitol Res. 2004 Mar;92(5):421-9
references: 14963773
references: Parasitol Res. 2008 Aug;103(3):671-83
references: 18543000
references: Turkiye Parazitol Derg. 2009;33(2):162-4
references: 19598095
references: Mol Biol Evol. 2007 Aug;24(8):1596-9
references: 17488738
references: Parasitol Res. 2012 Mar;110(3):1297-303
references: 21870244
references: Trends Parasitol. 2004 Oct;20(10):469-76
references: 15363440
references: Parasitology. 1998 Aug;117 ( Pt 2):155-64
references: 9778638
references: PLoS Negl Trop Dis. 2009;3(3):e397
references: 19308251
references: Parasitol Res. 1995;81(1):1-5
references: 7724508
references: Ann N Y Acad Sci. 2008 Dec;1149:368-70
references: 19120252