title: The role of cyclooxygenase-2 on endurance exercise training in female LDL-receptor knockout ovariectomized mice.
abstract: Estrogen deprivation in postmenopausal women increases cardiovascular risk. Cardiovascular risk as a result of atherosclerosis is able to induce an inflammatory disease as far as cyclooxygenase-2 ( COX-2) expression. The purpose of the study was to investigate the role of COX-2 on exercise training in female mice low-density lipoprotein receptor knockout ( LDL-KO) with or without ovariectomy. A total of 15 female C57BL/6 mice and 15 female LDL-KO mice were distributed into 6 groups: sedentary control, sedentary control ovariectomized, trained control ovariectomized, LDL-KO sedentary, LDL-KO sedentary ovariectomized and LDL-KO trained ovariectomized. The ascending part of the aorta was stained with H&E and COX-2 expression was assessed by immunohistochemistry. Results revealed that ovariectomy as well as exercise training were not able to induce histopathological changes in mouse aorta for all groups investigated. LDL-KO mice demonstrated plaque containing cholesterol clefts, foamy histiocytes and mild inflammatory process for all groups indistinctly. Ovariectomy induced a strong immunoexpression in atherosclerosis lesion of LDL-KO mice. Nevertheless, a down-regulation of COX-2 expression was detected in LDL-KO trained ovariectomized when compared to LDL-KO sedentary. Our results are consistent with the notion that exercise training is able to modulate COX-2 expression in LDL-KO mice as a result of COX-2 down-regulation.
authors: Departamento de Biociências, Universidade Federal de São Paulo, Campus Baixada Santista, 11060-001 Santos SP, Brasil. flavia.oliveira@unifesp.br
authors: Flavia
authors: F
authors: De Oliveira
authors: Laura B M
authors: LB
authors: Maifrino
authors: Gustavo P P
authors: GP
authors: De Jesus
authors: Juliana G
authors: JG
authors: Carvalho
authors: Cláudia
authors: C
authors: Marchon
authors: Daniel A
authors: DA
authors: Ribeiro
mesh_terms: D000818:Animals; D050197:Atherosclerosis / Q000378:metabolism*; D051546:Cyclooxygenase 2 / Q000378:metabolism*; D005260:Female; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D010052:Ovariectomy; D010805:Physical Conditioning, Animal / Q000502:physiology*; D010807:Physical Endurance / Q000502:physiology*; D011973:Receptors, LDL / Q000378:metabolism*
chemical_list: D011973:Receptors, LDL; D051546:Cyclooxygenase 2