title: Molecular and biological characterization of Trypanosoma cruzi strains isolated from children from Jequitinhonha Valley, State of Minas Gerais, Brazil.
abstract: BACKGROUND
  The biological diversity of Trypanosoma cruzi strains plays an important role in the clinical and epidemiological features of Chagas disease.
  
  
  METHODS
  Eight T. cruzi strains isolated from children living in a Chagas disease vector-controlled area of Jequitinhonha Valley, State of Minas Gerais, Brazil, were genetically and biologically characterized.
  
  
  RESULTS
  The characterizations demonstrated that all of the strains belonged to T. cruzi II, and showed high infectivity and a variable mean maximum peak of parasitemia. Six strains displayed low parasitemia, and two displayed moderate parasitemia. Later peaks of parasitemia and a predominance of intermediate and large trypomastigotes in all T. cruzi strains were observed. The mean pre-patent period was relatively short (4.2 ± 0.25 to 13.7 ± 3.08 days), whereas the patent period ranged from 3.3 ± 1.08 to 34.5 ± 3.52 days. Mortality was observed only in animals infected with strain 806 (62.5%). Histopathological analysis of the heart showed that strains 501 and 806 caused inflammation, but fibrosis was observed only in animals infected with strain 806.
  
  
  CONCLUSIONS
  The results indicate the presence of an association between the biological behavior in mice and the genetic characteristics of the parasites. The study also confirmed general data from Brazil where T. cruzi II lineage is the most prevalent in the domiciliary cycle and generally has low virulence, with some strains capable of inducing inflammatory processes and fibrosis.
authors: Núcleo de Pesquisas em Ciências Biológicas, Instituto de Ciências Exatas e Biológicas, Universidade Federal de Ouro Preto, Ouro Preto, MG. jackvalamiel@yahoo.com.br
authors: Jaquelline Carla Valamiel de Oliveira E
authors: JC
authors: Silva
authors: Girley Francisco Machado de
authors: GF
authors: Assis
authors: Maykon Tavares de
authors: MT
authors: Oliveira
authors: Helder Magno Silva
authors: HM
authors: Valadares
authors: Italo Faria do
authors: IF
authors: Valle
authors: Nivia Carolina Nogueira de
authors: NC
authors: Paiva
authors: Helen Rodrigues
authors: HR
authors: Martins
authors: Marta de
authors: Md
authors: Lana
mesh_terms: D000818:Animals; D001938:Brazil; D014355:Chagas Disease / Q000469:parasitology*; D002648:Child; D016054:DNA, Protozoan / Q000235:genetics; D004195:Disease Models, Animal; D005260:Female; D005838:Genotype; D006801:Humans; D051379:Mice; D018512:Parasitemia / Q000469:parasitology* / Q000473:pathology; D014349:Trypanosoma cruzi / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity*; D014774:Virulence
chemical_list: D016054:DNA, Protozoan