title: Comparative proteomics analysis of the rice roots colonized by Herbaspirillum seropedicae strain SmR1 reveals induction of the methionine recycling in the plant host.
abstract: Although the use of plant growth-promoting bacteria in agriculture is a reality, the molecular basis of plant-bacterial interaction is still poorly understood. We used a proteomic approach to study the mechanisms of interaction of Herbaspirillum seropedicae SmR1 with rice. Root proteins of rice seedlings inoculated or noninoculated with H. seropedicae were separated by 2-D electrophoresis. Differentially expressed proteins were identified by MALDI-TOF/TOF and MASCOT program. Among the identified proteins of H. seropedicae, the dinitrogenase reductase NifH and glutamine synthetase GlnA, which participate in nitrogen fixation and ammonium assimilation, respectively, were the most abundant. The rice proteins up-regulated included the S-adenosylmethionine synthetase, methylthioribose kinase, and acireductone dioxygenase 1, all of which are involved in the methionine recycling. S-Adenosylmethionine synthetase catalyzes the synthesis of S-adenosylmethionine, an intermediate used in transmethylation reactions and in ethylene, polyamine, and phytosiderophore biosynthesis. RT-qPCR analysis also confirmed that the methionine recycling and phytosiderophore biosynthesis genes were up-regulated, while ACC oxidase mRNA level was down-regulated in rice roots colonized by bacteria. In agreement with these results, ethylene production was reduced approximately three-fold in rice roots colonized by H. seropedicae. The results suggest that H. seropedicae stimulates methionine recycling and phytosiderophore synthesis and diminishes ethylene synthesis in rice roots.
authors: Department of Biochemistry and Molecular Biology, Federal University of Paraná , Rua Francisco H. dos Santos s/n Centro Politécnico, Curitiba, Paraná 81531-990, Brazil.
authors: Dayane
authors: D
authors: Alberton
authors: Marcelo
authors: M
authors: Müller-Santos
authors: Liziane Cristina Campos
authors: LC
authors: Brusamarello-Santos
authors: Glaucio
authors: G
authors: Valdameri
authors: Fabio Aparecido
authors: FA
authors: Cordeiro
authors: Marshall Geoffrey
authors: MG
authors: Yates
authors: Fabio
authors: F
authors: de Oliveira Pedrosa
authors: Emanuel Maltempi
authors: EM
authors: de Souza
mesh_terms: D017037:Dinitrogenase Reductase / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D005974:Glutamate-Ammonia Ligase / Q000378:metabolism; D042803:Herbaspirillum / Q000201:enzymology*; D008715:Methionine / Q000378:metabolism*; D008716:Methionine Adenosyltransferase / Q000378:metabolism; D012275:Oryza / Q000378:metabolism* / Q000382:microbiology*; D018517:Plant Roots / Q000382:microbiology*; D040901:Proteomics / Q000379:methods*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D036226:Seedlings / Q000378:metabolism; D017262:Siderophores / Q000096:biosynthesis; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013559:Symbiosis
chemical_list: D017037:Dinitrogenase Reductase; D017262:Siderophores; D008715:Methionine; D008716:Methionine Adenosyltransferase; D005974:Glutamate-Ammonia Ligase