title: Oxychlorine species suppress postsurgical adhesions in rats.
abstract: BACKGROUND
  Surgically induced adhesions complicate up to 100% of abdominal surgeries. Food and Drug Administration-approved treatments are generally not only less effective than desired but they also have major contraindications. Oxychlorine species, including chlorine dioxide (ClO2), suppress scar formation in infected wounds without affecting keratinocytes while reducing fibroblast proliferation. The aim of the present study was to evaluate the effect of oxychlorine solutions containing ClO2 on adhesion formation.
  
  
  METHODS
  Male Wistar rats were subjected to Buckenmaier model of surgical adhesions and treated with either oxychlorine solutions containing ClO2 (40-150 ppm) or isotonic saline solution. To increase the severity of adhesions, peritonitis was produced by intraperitoneal administration of a diluted nonlethal dose of feces (50 mg/kg). Wound strength of the healed wound was measured to evaluate the effects of oxychlorine solutions. In addition, an oxychlorine solution of lesser efficacy (at 100 ppm) was compared with three available anti-adhesion materials.
  
  
  RESULTS
  Reproducibility of the model was validated in 26 rats. Oxychlorine solutions containing ClO2 (40-110 ppm) significantly reduced postsurgical adhesion formation without affecting the strength of the healed wound. Higher concentrations (120 and 150 ppm) had no effect. Fecal peritonitis significantly increased, and solutions with ClO2 at 110 ppm significantly reduced adhesion formation. The effect of the oxychlorine solution was significantly greater than that of Interceed, Guardix, Seprafilm, and isotonic saline solution.
  
  
  CONCLUSIONS
  ClO2-containing oxychlorine solutions could be an innovative strategy for the suppression of surgical adhesion formation, with the additional advantage of contributing antiseptic properties.
authors: Departamento de Posgrado e Investigacion, Instituto Politecnico Nacional, Escuela Superior de Medicina, Mexico City, Mexico.
authors: Juan M
authors: JM
authors: Zavala-Rodriguez
authors: Jose M
authors: JM
authors: Correa Rovelo
authors: Noe
authors: N
authors: Martinez-Morales
authors: Carlos
authors: C
authors: Muñoz-Arce
authors: Rosa A
authors: RA
authors: Bobadilla-Lugo
authors: Robert D
authors: RD
authors: Kross
authors: Roberto
authors: R
authors: Medina
authors: Cleva
authors: C
authors: Villanueva
mesh_terms: D000818:Animals; D017606:Chlorine Compounds / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D000860:Hypoxia / Q000150:complications; D008297:Male; D010087:Oxides / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D015203:Reproducibility of Results; D000267:Tissue Adhesions / Q000209:etiology / Q000517:prevention & control*
chemical_list: D017606:Chlorine Compounds; D010087:Oxides; C025109:chlorine dioxide
keywords: Antisepsis; Chlorine dioxide; Free radical scavenger; Peritonitis; Surgical adhesions