title: [Effect of human colon carcinoma-associated fibroblasts on biological behavior of colon carcinoma Lovo cells].
abstract: OBJECTIVE
  To investigate the effects of human colon carcinoma-associated fibroblasts (CAFs) on proliferation, adhesion, migration and invasion of colon carcinoma Lovo cells.
  
  
  METHODS
  The co-culture models among colon CAFs, NFs and Lovo cell were established by conditioned medium (CM) of human colon CAFs and colon normal fibroblasts (NFs). Lovo cells in the blank control group was treated with serum-free culture medium. The effects of human colon CAFs on proliferation, adhesion, migration and invasion of colon carcinoma Lovo cells were detected by cell proliferation assay, adhesion assay, migration assay and Transwell invasion assay.
  
  
  RESULTS
  After co-culture with colon CAFs, the absorbance (A) value of Lovo cells was (0.667±0.059) in 48 h and (0.709±0.030) in 72 h. The A value of Lovo cells adhesion to fibronectin was (0.588±0.067). The cell mobility rates were (35.2±8.7)% in 12 h and (64.6±7.1)% in 24 h. The number of invasive cell was (56.2±4.8). All the above parameters were increased compared with those in the blank control group and NFs group (all P<0.01).
  
  
  CONCLUSIONS
  Human colon CAFs can promote the proliferation, adhesion, migration and invasion of colon carcinoma Lovo cells.
authors: Department of Pathology, The Affiliated Hospital of Inner Mongolia Medical University, Hohhot 010059, China. shilinsd2003@yahoo.com.cn.
authors: Xin-lin
authors: XL
authors: Wu
authors: Kai-jin
authors: KJ
authors: Lin
authors: Lin
authors: L
authors: Shi
authors: Xiu-lan
authors: XL
authors: Su
authors: Ming-xing
authors: MX
authors: Hou
authors: Pei-de
authors: PD
authors: Dong
mesh_terms: D002448:Cell Adhesion; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D018920:Coculture Techniques; D003110:Colonic Neoplasms / Q000473:pathology*; D005347:Fibroblasts / Q000166:cytology*; D006801:Humans