title: Isochronal visualization of transcription and proteasomal proteolysis in cell culture or in the model organism, Caenorhabditis elegans.
abstract: Investigation of differential gene regulation by protein degradation requires analysis of the spatial and temporal association between proteolysis and transcription. Here, we describe the isochronal visualization of proteasomal proteolysis and transcription in cell culture or in vivo in the model organism Caenorhabditis elegans. This includes localization of proteasome-dependent proteolysis by fluorescent degradation products of model and endogenous substrates of the proteasome in combination with immunolabelling of RNA polymerase II and transcription in situ run-on assays.
authors: IUF - Leibniz Research Institute for Environmental Medicine at Heinrich-Heine University Düsseldorf, Düsseldorf, Germany.
authors: Anna
authors: A
authors: von Mikecz
authors: Andrea
authors: A
authors: Scharf
mesh_terms: D000818:Animals; D017173:Caenorhabditis elegans / Q000235:genetics / Q000378:metabolism; D045744:Cell Line, Tumor; D005786:Gene Expression Regulation / Q000235:genetics; D056945:Hep G2 Cells; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D059748:Proteolysis; D012319:RNA Polymerase II / Q000276:immunology; D014158:Transcription, Genetic; D054875:Ubiquitination / Q000235:genetics
chemical_list: D012319:RNA Polymerase II