title: Antihyperalgesic and antiedematous activities of bisabolol-oxides-rich matricaria oil in a rat model of inflammation.
abstract: From the dried flower heads of Matricaria recutita L., essential oil was isolated by hydrodistillation, and in the obtained blue oil, α-bisabolol oxide A (21.5%), α-bisabolol oxide B (25.5%) and (Z)-spiroether (cis-en-yn-spiroether) (10.3%) were identified as the main compounds, by gas chromatography (GC) and GC-mass spectrometry analyses. The antihyperalgesic effects of this oil were examined in a rat model of inflammation induced by carrageenan, through a modified 'paw-pressure' test. Antiedematous effects were examined in a rat model of inflammation induced by carrageenan, dextran and histamine, through plethysmometry. Matricaria oil (25, 50 and 100 mg/kg, p.o.) exhibited a significant dose-dependent reduction of hyperalgesia and edema induced by carrageenan in both prophylactic and therapeutic treatment schemes. It was more efficacious in the prophylactic treatment scheme, and the corresponding median effective dose (ED50 ) ± standard error of the mean (SEM) values were 49.8 ± 6.0 and 42.4 ± 0.2 mg/kg for antihyperalgesic and antiedematous effects, respectively. Prophylactic treatments with matricaria oil (25, 50 and 100 mg/kg, p.o.) caused a significant dose-dependent antiedematous effect in dextran-induced edema with lower efficacy than in the carrageenan model. In a dose of 100 mg/kg, p.o., matricaria oil caused a slight reduction of histamine-induced edema. These results suggest that bisabolol-oxide-rich matricaria oil may be effective against pain and edema present in various inflammatory conditions, which supports matricaria traditional uses.
authors: Department of Pharmacology, Faculty of Pharmacy, University of Belgrade, Vojvode Stepe 450, 11221, Belgrade, Serbia.
authors: Maja
authors: M
authors: Tomić
authors: Višnja
authors: V
authors: Popović
authors: Silvana
authors: S
authors: Petrović
authors: Radica
authors: R
authors: Stepanović-Petrović
authors: Ana
authors: A
authors: Micov
authors: Milica
authors: M
authors: Pavlović-Drobac
authors: Maria
authors: M
authors: Couladis
mesh_terms: D000700:Analgesics / Q000737:chemistry / Q000494:pharmacology*; D000818:Animals; D004305:Dose-Response Relationship, Drug; D004487:Edema / Q000188:drug therapy*; D004988:Ethers, Cyclic / Q000737:chemistry / Q000494:pharmacology; D008401:Gas Chromatography-Mass Spectrometry; D006930:Hyperalgesia / Q000188:drug therapy*; D007249:Inflammation / Q000188:drug therapy; D008297:Male; D031168:Matricaria / Q000737:chemistry*; D000081222:Monocyclic Sesquiterpenes; D009822:Oils, Volatile / Q000737:chemistry / Q000494:pharmacology*; D010087:Oxides / Q000737:chemistry; D010146:Pain / Q000188:drug therapy; D010938:Plant Oils / Q000737:chemistry / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D012717:Sesquiterpenes / Q000737:chemistry / Q000494:pharmacology*; D013141:Spiro Compounds / Q000737:chemistry / Q000494:pharmacology
chemical_list: D000700:Analgesics; D004988:Ethers, Cyclic; D000081222:Monocyclic Sesquiterpenes; D009822:Oils, Volatile; D010087:Oxides; D010938:Plant Oils; D012717:Sesquiterpenes; D013141:Spiro Compounds; C531272:spiroether; C004497:bisabolol
keywords: Matricaria recutita essential oil; antiedematous activity; antihyperalgesic activity; bisabolol oxides type; localized inflammation