title: Isolation of Genes Specifically Expressed in Different Developmental Stages of Pleurotus ostreatus Using Macroarray Analysis.
abstract: The oyster mushroom (Pleurotus ostreatus) is one of the most important edible mushrooms worldwide. The mechanism of P. ostreatus fruiting body development has been of interest both for the basic understanding of the phenotypic change of the mycelium-fruiting body and to improve breeding of the mushrooms. Based on our previous publication of P. ostreatus expressed sequence tag database, 1,528 unigene clones were used in macroarray analysis of mycelium, fruiting body and basidiospore developmental stages of P. ostreatus. Gene expression profile databases generated by evaluating expression levels showed that 33, 10, and 94 genes were abundantly expressed in mycelium, fruiting body and basidiospore developmental stages, respectively. Among them, the genes specifically expressed in the fruiting body stage were further analyzed by reverse transcription-polymerase chain reaction and Northern blot to investigate temporal and spatial expression patterns. These results provide useful information for future studies of edible mushroom development.
authors: Bio-crops Development Division, National Academy of Agricultural Science, RDA, Suwon, Korea.
authors: Seung-Ho
authors: SH
authors: Lee
authors: Joong-Ho
authors: JH
authors: Joh
authors: Jin-Sung
authors: JS
authors: Lee
authors: Jong-Hyun
authors: JH
authors: Lim
authors: Kyung-Yun
authors: KY
authors: Kim
authors: Young-Bok
authors: YB
authors: Yoo
authors: Chang-Soo
authors: CS
authors: Lee
authors: Beom-Gi
authors: BG
authors: Kim
keywords: Fruiting body development; Oyster mushroom; Pleurotus ostreatus; Specific gene expression
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