title: Spontaneous calcium waves in granule cells in cerebellar slice cultures.
abstract: Multiple regions in the CNS display propagating correlated activity during embryonic and postnatal development. This activity can be recorded as waves of increased calcium concentrations in spiking neurons or glia cells, and have been suggested to be involved in patterning, axonal guidance and establishment of synaptic transmission. Here, we used calcium imaging in slice cultures of the postnatal cerebellum, and observe spontaneous propagating calcium waves in NeuN-positive granule-like cells. Wave formation was blocked by TTX and the AMPA antagonist NBQX, but persisted after NMDA receptor blockade with MK-801. Whole-cell recordings during wave formation showed cyclic EPSP barrages with an amplitude of 10-20 mV concurrent with wave activity. Local non-propagating putative transglial waves were also present in the cultures, and could be reproduced by pressure application of ATP. We hypothesize, that the propagating wave activity is carried through the tissue by axonal collaterals formed by neighboring granule cells, and further suggest that the correlated activity may be related to processes that ensure correct postnatal wiring of the cerebellar circuits.
authors: Department of Neuroscience and Pharmacology, Copenhagen University, Panum Institute, 12.3, Blegdamsvej 3, DK-2200 Copenhagen N, Denmark. Electronic address: miaapuschkin@gmail.com.
authors: Mia
authors: M
authors: Apuschkin
authors: Maria
authors: M
authors: Ougaard
authors: Jens C
authors: JC
authors: Rekling
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002531:Cerebellum / Q000166:cytology / Q000378:metabolism*; D066298:In Vitro Techniques; D051379:Mice; D009474:Neurons / Q000378:metabolism*; D018408:Patch-Clamp Techniques; D018091:Receptors, AMPA / Q000378:metabolism; D009435:Synaptic Transmission
chemical_list: D018091:Receptors, AMPA; D002118:Calcium
keywords: Axon guidance; Calcium waves; Cerebellum; Granule cells; Patterning