title: Gender-specific transcriptional profiling of marine medaka (Oryzias melastigma) liver upon BDE-47 exposure.
abstract: Marine medaka (Oryzias melastigma) were exposed to 2,2',4,4'-tetrabromodiphenyl ether (BDE-47) to investigate the gender-specific transcriptional profiles of liver tissue in response to this flame retardant. A cDNA library of O. melastigma was constructed, and 2304 clones were amplified from the library to fabricate a cDNA microarray. Sequences of these genes were assembled into 1800 sequences using Geneious, a bioinformatics software. Corresponding expressed sequence tags were blasted against the National Centre for Biotechnology Information non-redundant database and further classified into various biological categories according to the Gene Ontology project. Male and female three-month-old were fed a diet of BDE-47 contaminated Artemia at low dosage (290.3±172.3ng BDE-47/day) and high dosage (580.5±344.6ng BDE-47/day) for 5 and 21 days, respectively. The transcriptional profiles of O. melastigma liver were then generated by the species-specific cDNA microrarray. The results from microarray analysis suggested very different gene expression patterns between males and females for both BDE-47 exposure-dose and exposure-time, with male livers having stronger gene regulatory responses than female livers. Importantly, our results revealed that in male O. melastigma only, BDE-47 exposure may activate phosphoinositide-3-kinase and mitogen-activated protein kinase, proteins that play importance roles in cell growth, proliferation and survival.
authors: State Key Laboratory in Marine Pollution, Department of Biology and Chemistry, City University of Hong Kong, Tat Chee Avenue, Kowloon, Hong Kong, China.
authors: W K
authors: WK
authors: Yu
authors: Y F
authors: YF
authors: Shi
authors: C C
authors: CC
authors: Fong
authors: Y
authors: Y
authors: Chen
authors: J P
authors: JP
authors: van de Merwe
authors: Alice K Y
authors: AK
authors: Chan
authors: F
authors: F
authors: Wei
authors: J
authors: J
authors: Bo
authors: R
authors: R
authors: Ye
authors: Doris W T
authors: DW
authors: Au
authors: Rudolf S S
authors: RS
authors: Wu
authors: M S
authors: MS
authors: Yang
mesh_terms: D000284:Administration, Oral; D000818:Animals; D001156:Artemia / Q000737:chemistry; D016000:Cluster Analysis; D004032:Diet; D005260:Female; D029941:Fish Proteins / Q000737:chemistry / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D020869:Gene Expression Profiling / Q000379:methods*; D055768:Halogenated Diphenyl Ethers / Q000633:toxicity*; D008099:Liver / Q000737:chemistry* / Q000378:metabolism; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis; D009990:Oryzias / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D012737:Sex Factors; D015398:Signal Transduction; D059467:Transcriptome
chemical_list: D029941:Fish Proteins; D055768:Halogenated Diphenyl Ethers; C511295:2,2',4,4'-tetrabromodiphenyl ether
keywords: -omics; AGXT2L1; AKT; ALDOB; ATP synthase; ATP5A1; BDE-47; COX; F10; FBG; FDPS; FGG; GOT2; H+transporting; HDAC8; IGFBP; KING1; MYCL1; Oryzias melastigma; PNP; SNF1-relatedprotein kinase regulation subunit gamma 1; SSR1; TF; alanine-glyoxylate aminotransferase 2-like 1; aldolase B; coagulation factor X; cyclooxygenase; farnesyl pyrophosphate synthetase; fibrinogen; fibrinogen gamma chain; glutamic oxaloacetic transaminase 2 (aspartate aminotransferase 2); histone deacetylase 8; insulin-like growth factor-binding protein 1; mitochondrial F1 complex, alpha subunit 1; protein kinase B; purine nucleoside phosphorylase; signal sequence receptor, alpha 1; transcription factor; v-mycmyelocytomatosis viral oncogene homolog 1