title: Exercise reduces cellular stress related to skeletal muscle insulin resistance.
abstract: This study sought to evaluate the effects of a single session of exercise on the expression of Hsp70, of c-jun N-terminal kinase (JNK), and insulin receptor substrate 1 serine 612 (IRS(ser612)) phosphorylation in the skeletal muscle of obese and obese insulin-resistant patients. Twenty-seven volunteers were divided into three experimental groups (eutrophic insulin-sensitive, obese insulin-sensitive, and obese insulin-resistant) according to their body mass index and the presence of insulin resistance. The volunteers performed 60 min of aerobic exercise on a cycle ergometer at 60 % of peak oxygen consumption. M. vastus lateralis samples were obtained before and after exercise. The protein expressions were evaluated by Western blot. Our findings show that compared with paired eutrophic controls, obese subjects have higher basal levels of p-JNK (100 ± 23 % vs. 227 ± 67 %, p = 0.03) and p-IRS-1(ser612) (100 ± 23 % vs. 340 ± 67 %, p < 0.001) and reduced HSP70 (100 ± 16 % vs. 63 ± 12 %, p < 0.001). The presence of insulin resistance results in a further increase in p-JNK (460 ± 107 %, p < 0.001) and a decrease in Hsp70 (46 ± 5 %, p = 0.006), but p-IRS-1(ser612) levels did not differ from obese subjects (312 ± 73 %, p > 0.05). Exercise reduced p-JNK in obese insulin-resistant subjects (328 ± 33 %, p = 0.001), but not in controls or obese subjects. Furthermore, exercise reduced p-IRS-1(ser612) for both obese (122 ± 44 %) and obese insulin-resistant (185 ± 36 %) subjects. A main effect of exercise was observed in HSP70 (p = 0.007). We demonstrated that a single session of exercise promotes changes that characterize a reduction in cellular stress that may contribute to exercise-induced increase in insulin sensitivity.
authors: Exercise Physiology Laboratory, Healthy and Biological Sciences Faculty, Federal University of Jequitinhonha and Mucuri Valleys, Rua da Glória 187, Diamantina, 39100-100, Minas Gerais, Brazil.
authors: Mariana Aguiar
authors: MA
authors: de Matos
authors: Vinícius de Oliveira
authors: Vde O
authors: Ottone
authors: Tamiris Campos
authors: TC
authors: Duarte
authors: Pâmela Fiche da Matta
authors: PF
authors: Sampaio
authors: Karine Beatriz
authors: KB
authors: Costa
authors: Cheyenne Alves
authors: CA
authors: Fonseca
authors: Miguel Pontes Correa
authors: MP
authors: Neves
authors: Suzanne Maria
authors: SM
authors: Schneider
authors: Pope
authors: P
authors: Moseley
authors: Cândido Celso
authors: CC
authors: Coimbra
authors: Flávio de Castro
authors: Fde C
authors: Magalhães
authors: Etel
authors: E
authors: Rocha-Vieira
authors: Fabiano Trigueiro
authors: FT
authors: Amorim
mesh_terms: D000328:Adult; D015444:Exercise; D005215:Fasting / Q000097:blood; D005260:Female; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism; D006801:Humans; D007328:Insulin / Q000097:blood; D055504:Insulin Receptor Substrate Proteins / Q000378:metabolism; D007333:Insulin Resistance; D048031:JNK Mitogen-Activated Protein Kinases / Q000378:metabolism; D008297:Male; D009124:Muscle Proteins / Q000378:metabolism; D018482:Muscle, Skeletal / Q000201:enzymology / Q000473:pathology*; D010766:Phosphorylation; D010768:Phosphoserine / Q000378:metabolism; D013312:Stress, Physiological
chemical_list: D018840:HSP70 Heat-Shock Proteins; D007328:Insulin; D055504:Insulin Receptor Substrate Proteins; D009124:Muscle Proteins; D010768:Phosphoserine; D048031:JNK Mitogen-Activated Protein Kinases
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