title: Insertion sequenceAS5(ISAS5) is involved in the genomic plasticity ofAeromonas salmonicida.
abstract: The genome of the fish pathogenAeromonas salmonicidasubspsalmonicidaharbors a large number of insertion sequences (ISs), many of which are located on plasmids. In the present study, we analyzed the small plasmid profile ofA. salmonicidastrains to identify evidences of plasmid alterations. Ten out of 78 strains analyzed displayed an unconventional plasmid profile. However the HER1104 strain was unique, having a positive PCR signal for pAsal1 plasmid despite not carrying this plasmid. Instead, HER1104 was bearing a plasmid at higher molecular weight than pAsal1. We characterized this new larger plasmid, which we called pAsal1B since it is a derivative of pAsal1 containing one more complete IS (ISAS5) than the parental plasmid. An additional 96 bp relic of ISAS5was also present in pAsal1B. These results propose that ISAS5is another active mobile genetic element inA. salmonicidasubspsalmonicidaand provided further proof of the genomic plasticity of this bacterium.
authors: Institut de Biologie Intégrative et des Systèmes (IBIS); Pavillon Charles-Eugène-Marchand; Université Laval; Quebec City, QC Canada ; Centre de Recherche de l'Institut Universitaire de Cardiologie et de Pneumologie de Québec; Quebec City, QC Canada.
authors: Mélanie V
authors: MV
authors: Trudel
authors: Katherine H
authors: KH
authors: Tanaka
authors: Geneviève
authors: G
authors: Filion
authors: Rana K
authors: RK
authors: Daher
authors: Michel
authors: M
authors: Frenette
authors: Steve J
authors: SJ
authors: Charette
keywords: Aeromonas salmonicida; DNA rearrangement; furunculosis; insertion sequence; plasmid
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