title: Relationship between severity of adult community-acquired pneumonia and impairment of the antioxidant defense system.
abstract: Oxidant/antioxidant imbalance has been reported in some infectious diseases, including community-acquired pneumonia (CAP). The aim was to assess the antioxidant status in adults with CAP and its relationship with clinical severity at admission. Fifty-nine patients with CAP were enrolled and categorized at admission by the FINE score, from July 2010 to October 2012. In the same period 61 controls were enrolled. Plasma samples were obtained at admission for determination of the ferric reducing ability of plasma (FRAP) and lipid peroxidation (8-isoprostane). Erythrocyte reduced (GSH)/oxidized (GSSG) glutathione, malondialdehyde (MDA) and antioxidant enzyme activity were assessed. Antioxidant status in adults with CAP represented by FRAP and the GSH/GSSG ratio were 16.8% (p=0.03) and 39.7% (p=0.04) lower than control values, respectively. In addition, FRAP values showed a positive correlation with GSH/GSSG ratio (r=0.852; p<0.02; n=59). The CAP group showed greater lipid peroxidation in both plasma and erythrocytes. The FINE score correlated negatively with FRAP (r= -0.718; p<0.05; n=59) and positively with MDA and F2 isoprostane levels (r=0.673; p<0.05; n=59; r=0.892; p<0.01; n=59, respectively). Antioxidant status alterations correlated with clinical severity. The FRAP assay and lipid peroxidation biomarkers may provide a useful parameter for estimating the severity and the clinical outcome of patients with CAP.
authors: Pathophysiology Program, Faculty of Medicine, University of Chile, Chile. rcastillo@med.uchile.cl
authors: Rodrigo L
authors: RL
authors: Castillo
authors: Rodrigo A
authors: RA
authors: Carrasco
authors: Pedro I
authors: PI
authors: Alvarez
authors: Mauricio
authors: M
authors: Ruiz
authors: Vivian
authors: V
authors: Luchsinger
authors: Enna
authors: E
authors: Zunino
authors: María A
authors: MA
authors: Martínez
authors: Luis F
authors: LF
authors: Avendaño
mesh_terms: D000368:Aged; D015415:Biomarkers / Q000378:metabolism; D016022:Case-Control Studies; D002374:Catalase / Q000097:blood / Q000378:metabolism; D017714:Community-Acquired Infections / Q000378:metabolism; D003430:Cross-Sectional Studies; D004912:Erythrocytes / Q000378:metabolism*; D028441:F2-Isoprostanes / Q000097:blood; D005260:Female; D005978:Glutathione / Q000097:blood*; D005979:Glutathione Peroxidase / Q000097:blood / Q000378:metabolism; D006801:Humans; D015227:Lipid Peroxidation / Q000502:physiology*; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D008875:Middle Aged; D018384:Oxidative Stress / Q000502:physiology*; D011014:Pneumonia / Q000378:metabolism*; D012720:Severity of Illness Index; D013482:Superoxide Dismutase / Q000097:blood / Q000378:metabolism
chemical_list: D015415:Biomarkers; D028441:F2-Isoprostanes; D008315:Malondialdehyde; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005978:Glutathione