title: Covalent attachment of Mn-porphyrin onto doxorubicin-loaded poly(lactic acid) nanoparticles for potential magnetic resonance imaging and pH-sensitive drug delivery.
abstract: In this paper, theranostic nanoparticles (MnP-DOX NPs) were fabricated by conjugating Mn-porphyrin onto the surface of doxorubicin (DOX)-loaded poly(lactic acid) (PLA) nanoparticles (DOX NPs) for potential T1 magnetic resonance imaging and pH-sensitive drug delivery. An in vitro drug release study showed that the release rate of DOX from MnP-DOX NPs was slow at neutral pH but accelerated significantly in acidic conditions. It was found that MnP-DOX NPs could be easily internalized by HeLa cells and effectively suppressed the growth of HeLa cells and HT-29 cells due to the accelerated drug release in acidic lysosomal compartments. Magnetic resonance imaging (MRI) scanning analysis demonstrated that MnP-DOX NPs had much higher longitudinal relaxivity in water (r1 value of 27.8 mM(-1) s(-1) of Mn(3+)) than Mn-porphyrin (Mn(III)TPPS3NH2; r1 value of 6.70 mM(-1) s(-1) of Mn(3+)), behaving as an excellent contrast agent for T1-weighted MRI both in vitro and in vivo. In summary, such a smart and promising nanoplatform integrates multiple capabilities for effective cancer diagnosis and therapy.
authors: School of Life Science and Technology, Harbin Institute of Technology, Harbin 150001, China.
authors: Lijia
authors: L
authors: Jing
authors: Xiaolong
authors: X
authors: Liang
authors: Xiaoda
authors: X
authors: Li
authors: Yongbo
authors: Y
authors: Yang
authors: Zhifei
authors: Z
authors: Dai
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology; D001672:Biocompatible Materials / Q000494:pharmacology; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism; D048430:Cell Shape / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004317:Doxorubicin / Q000737:chemistry / Q000494:pharmacology*; D016503:Drug Delivery Systems; D004705:Endocytosis / Q000187:drug effects; D019073:HT29 Cells; D006367:HeLa Cells; D061307:Human Umbilical Vein Endothelial Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D006863:Hydrogen-Ion Concentration / Q000187:drug effects; D019344:Lactic Acid / Q000737:chemistry*; D008279:Magnetic Resonance Imaging; D008665:Metalloporphyrins / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D011091:Polyesters; D011108:Polymers / Q000737:chemistry*; D011166:Porphyrins / Q000737:chemistry*; D013347:Subcellular Fractions / Q000187:drug effects / Q000378:metabolism
chemical_list: D000970:Antineoplastic Agents; D001672:Biocompatible Materials; D008665:Metalloporphyrins; D011091:Polyesters; D011108:Polymers; D011166:Porphyrins; D019344:Lactic Acid; C033616:poly(lactide); D004317:Doxorubicin
keywords: Doxorubicin; MRI; Mn-porphyrin; Theranostic agent; pH-sensitive drug delivery