title: MS-based monitoring of proteolytic decay of synthetic reporter peptides for quality control of plasma and serum specimens.
abstract: OBJECTIVE
  To determine the preanalytical quality of serum and plasma by monitoring the time-dependent ex vivo decay of a synthetic reporter peptide (RP) with liquid chromatography/mass spectrometry (LC/MS).
  
  
  METHODS
  Serum and plasma specimens were spiked with the RP and proteolytic fragments were monitored with LC/MS at different preanalytical time points ranging from 2 to 24 hours after blood withdrawal.
  
  
  RESULTS
  The concentration of fragments changed in a time-dependent manner, and respective peptide profiles were used to classify specimens according to their preanalytical time span. Classification accuracy was high, with values always above 0.89 for areas under receiver operating characteristic curves.
  
  
  CONCLUSIONS
  This "proteomics degradation clock" can be used to estimate the preanalytical quality of serum and plasma and might have impact on quality control procedures of biobanking repositories.
authors: Institute for Clinical Chemistry, Medical Faculty Mannheim of the University of Heidelberg, Theodor-Kutzer-Ufer 1, 68167 Mannheim, Germany. peter.findeisen@umm.de
authors: Peter
authors: P
authors: Findeisen
authors: Jörg Oliver
authors: JO
authors: Thumfart
authors: Victor
authors: V
authors: Costina
authors: Ralf
authors: R
authors: Hofheinz
authors: Michael
authors: M
authors: Neumaier
mesh_terms: D001800:Blood Specimen Collection / Q000379:methods*; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D010455:Peptides / Q000032:analysis*; D059748:Proteolysis; D040901:Proteomics / Q000379:methods*; D011786:Quality Control
chemical_list: D010455:Peptides
keywords: Biobanking; Decay; Mass spectrometry; Plasma; Protease; Quality assessment; Reporter peptides; Serum