title: Transmenopausal changes in trabecular bone quality.
abstract: Bone strength depends on its amount and quality. Bone quality includes its structural and material properties. Bone material properties are dependent on bone turnover rates. Remodeling rates are significantly increased immediately after menopause. In the present study, we used Raman microspectroscopic analysis of double iliac crest biopsies with a spatial resolution of 1 µm obtained before and immediately after menopause (1 year after cessation of menses) in healthy females to investigate changes in material properties attributable to menopause. In particular, the mineral/matrix ratio, the relative proteoglycan and lipid content, the mineral maturity/crystallinity, and the relative pyridinoline collagen cross-link content were determined in trabecular bone as a function of surface metabolic activity and tissue age. The results indicate that significant changes (specifically in mineral/matrix ratio) were evident at active bone forming surfaces, whereas the relative proteoglycan content was altered at resorbing surfaces. These changes were not accompanied by altered mineral content or quality as monitored by Raman microspectroscopic analysis.
authors: Ludwig Boltzmann Institute of Osteology, Hanusch Hospital, Vienna, Austria.
authors: Sonja
authors: S
authors: Gamsjaeger
authors: Wolfgang
authors: W
authors: Brozek
authors: Robert
authors: R
authors: Recker
authors: Klaus
authors: K
authors: Klaushofer
authors: Eleftherios P
authors: EP
authors: Paschalis
mesh_terms: D001842:Bone and Bones / Q000033:anatomy & histology*; D005260:Female; D005456:Fluorescent Dyes; D006801:Humans; D008593:Menopause; D008875:Middle Aged; D013059:Spectrum Analysis, Raman
chemical_list: D005456:Fluorescent Dyes
keywords: BONE QUALITY; MENOPAUSE; RAMAN MICROSPECTROSCOPIC ANALYSIS