title: Identification of miR-106b-93 as a negative regulator of brown adipocyte differentiation.
abstract: microRNAs (miRNAs) have been reported to play an essential role in the regulation of brown adipocyte adipogenesis. In the present study, we investigated the role of the miR-106b-93 cluster in the differentiation of brown adipocytes. We found that knockdown of miR-106b and miR-93 significantly induced the expression of brown fat-specific genes and promoted the accumulation of lipid-droplet in differentiating brown adipocytes. In addition, ectopic expression of miR-106b and miR-93 suppressed the mRNA level of Ucp1, a selective hallmark of brown adipocytes. Furthermore, the expression levels of miR-106b and miR-93 are higher in brown adipose tissues of high fat diet-induced obese mice compared to control mice. Taken together, our results identify miR-106b and miR-93 as negative regulators of brown adipocyte differentiation and the miR-106b-93 cluster may play an important role in regulating energy homeostasis.
authors: Second Xiangya Hospital, Metabolic Syndrome Research Center and Diabetes Center, Institute of Aging and Geriatric Diseases, Key Laboratory of Diabetes Immunology, Ministry of Education, Central South University, Hunan, China.
authors: Yan
authors: Y
authors: Wu
authors: Jinrong
authors: J
authors: Zuo
authors: Yacheng
authors: Y
authors: Zhang
authors: Yin
authors: Y
authors: Xie
authors: Fang
authors: F
authors: Hu
authors: Lihui
authors: L
authors: Chen
authors: Bilian
authors: B
authors: Liu
authors: Feng
authors: F
authors: Liu
mesh_terms: D052437:Adipocytes, Brown / Q000166:cytology* / Q000378:metabolism; D050156:Adipogenesis; D000818:Animals; D002460:Cell Line; D059305:Diet, High-Fat; D018507:Gene Expression Regulation, Developmental; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008820:Mice, Obese; D035683:MicroRNAs / Q000032:analysis / Q000235:genetics*; D014280:Triglycerides / Q000032:analysis / Q000378:metabolism; D015854:Up-Regulation
chemical_list: D035683:MicroRNAs; C543896:Mirn106 microRNA, mouse; C551470:Mirn93 microRNA, mouse; D014280:Triglycerides
keywords: Brown adipocytes; Differentiation; MicroRNA; miR-106b; miR-93