title: Establishment of a new murine model of liver abscess induced by Fusobacterium necrophorum injected into the caudal vein.
abstract: Anaerobic bacterial infection is often accompanied by abscess formation; however, few in vivo studies have been published with descriptive data specifically evaluating antimicrobial activity in the presence of abscesses. The aim of this study was to establish a murine model of anaerobic infection with abscess formation and to verify the utility of this model for evaluating the in vivo efficacy of an antimicrobial agent. A clinical isolate of Fusobacterium necrophorum was inoculated into the caudal vein of immunocompetent BALB/c mice at 10(8) c.f.u. per mouse. Changes in body weight, bacterial load and histopathology of key organs were evaluated. After inoculation, bacterial counts in the liver increased from 10(4) to 10(8) c.f.u. after 1-3 days, and liver abscess formation was observed on the day following infection. Abscess formation and bacterial growth were not observed in other organs. In this model, 3 days of treatment with 5 mg metronidazole kg(-1) eradicated F. necrophorum in the liver; however, a reduction in bacterial load was not observed with 0.05 mg metronidazole kg(-1). In this study, we established a novel murine model of F. necrophorum liver abscess via haematogenous infection that may be useful for investigating in vivo antimicrobial activity against anaerobic abscesses and understanding the pathogenesis of F. necrophorum infection.
authors: First Department of Internal Medicine, Hokkaido University Hospital, Hokkaido, Japan.|Second Department of Internal Medicine, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan.|Department of Laboratory Medicine, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan.
authors: Kentaro
authors: K
authors: Nagaoka
authors: Department of Laboratory Medicine, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan.
authors: Katsunori
authors: K
authors: Yanagihara
authors: Second Department of Internal Medicine, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan.|Department of Laboratory Medicine, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan.
authors: Yosuke
authors: Y
authors: Harada
authors: Second Department of Internal Medicine, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan.|Department of Laboratory Medicine, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan.
authors: Koichi
authors: K
authors: Yamada
authors: Second Department of Internal Medicine, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan.|Department of Laboratory Medicine, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan.
authors: Yohei
authors: Y
authors: Migiyama
authors: Second Department of Internal Medicine, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan.|Department of Laboratory Medicine, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan.
authors: Yoshitomo
authors: Y
authors: Morinaga
authors: Second Department of Internal Medicine, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan.
authors: Koichi
authors: K
authors: Izumikawa
authors: Second Department of Internal Medicine, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan.
authors: Hiroshi
authors: H
authors: Kakeya
authors: Department of Tumor and Diagnostic Pathology, Atomic Bomb Disease Institute, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan.
authors: Masahiro
authors: M
authors: Nakashima
authors: First Department of Internal Medicine, Hokkaido University Hospital, Hokkaido, Japan.
authors: Masaharu
authors: M
authors: Nishimura
authors: Global COE Program, Nagasaki University, Nagasaki, Japan.|Second Department of Internal Medicine, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan.
authors: Shigeru
authors: S
authors: Kohno
mesh_terms: D061605:Administration, Intravenous; D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage; D058491:Bacterial Load; D001835:Body Weight; D004195:Disease Models, Animal; D005674:Fusobacterium Infections / Q000382:microbiology* / Q000473:pathology*; D005675:Fusobacterium necrophorum / Q000472:pathogenicity*; D006651:Histocytochemistry; D008099:Liver / Q000382:microbiology / Q000473:pathology; D008100:Liver Abscess / Q000382:microbiology* / Q000473:pathology*; D008297:Male; D008795:Metronidazole / Q000008:administration & dosage; D051379:Mice; D008807:Mice, Inbred BALB C
chemical_list: D000900:Anti-Bacterial Agents; D008795:Metronidazole