title: Cooperation between Rho-GEF Gef2 and its binding partner Nod1 in the regulation of fission yeast cytokinesis.
abstract: Cytokinesis is the last step of the cell-division cycle, which requires precise spatial and temporal regulation to ensure genetic stability. Rho guanine nucleotide exchange factors (Rho GEFs) and Rho GTPases are among the key regulators of cytokinesis. We previously found that putative Rho-GEF Gef2 coordinates with Polo kinase Plo1 to control the medial cortical localization of anillin-like protein Mid1 in fission yeast. Here we show that an adaptor protein, Nod1, colocalizes with Gef2 in the contractile ring and its precursor cortical nodes. Like gef2, nod1 has strong genetic interactions with various cytokinesis mutants involved in division-site positioning, suggesting a role of Nod1 in early cytokinesis. We find that Nod1 and Gef2 interact through the C-termini, which is important for their localization. The contractile-ring localization of Nod1 and Gef2 also depends on the interaction between Nod1 and the F-BAR protein Cdc15, where the Nod1/Gef2 complex plays a role in contractile-ring maintenance and affects the septation initiation network. Moreover, Gef2 binds to purified GTPases Rho1, Rho4, and Rho5 in vitro. Taken together, our data indicate that Nod1 and Gef2 function cooperatively in a protein complex to regulate fission yeast cytokinesis.
authors: Department of Molecular Genetics, The Ohio State University, Columbus, OH 43210 Department of Molecular and Cellular Biochemistry, The Ohio State University, Columbus, OH 43210 Department of Chemistry and Biochemistry, The Ohio State University, Columbus, OH 43210.
authors: Yi-Hua
authors: YH
authors: Zhu
authors: Yanfang
authors: Y
authors: Ye
authors: Zhengrong
authors: Z
authors: Wu
authors: Jian-Qiu
authors: JQ
authors: Wu
mesh_terms: D000205:Actomyosin / Q000235:genetics / Q000378:metabolism; D018797:Cell Cycle Proteins / Q000235:genetics* / Q000378:metabolism; D048749:Cytokinesis / Q000235:genetics*; D019204:GTP-Binding Proteins / Q000378:metabolism; D020662:Guanine Nucleotide Exchange Factors / Q000737:chemistry / Q000235:genetics*; D046912:Multiprotein Complexes / Q000737:chemistry / Q000235:genetics*; D009154:Mutation; D011485:Protein Binding; D012568:Schizosaccharomyces / Q000235:genetics; D029702:Schizosaccharomyces pombe Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D015398:Signal Transduction; D020741:rho GTP-Binding Proteins / Q000378:metabolism
chemical_list: C082279:CDC15 protein; D018797:Cell Cycle Proteins; C582271:Gef2 protein, S pombe; D020662:Guanine Nucleotide Exchange Factors; D046912:Multiprotein Complexes; C582313:Nod1 protein, S pombe; D029702:Schizosaccharomyces pombe Proteins; D000205:Actomyosin; D019204:GTP-Binding Proteins; D020741:rho GTP-Binding Proteins
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