title: Quantification of human kallikrein-2 in clinical samples by selected reaction monitoring.
abstract: Recently, the number of mass spectrometry-based quantification assays has been increased, partially due to the global efforts of chromosome-centric human proteome project (C-HPP). Our goal at the Chromosome 19 Consortium is to provide novel selected reaction monitoring (SRM) assays of proteins coded on chromosome 19. We have selected the two most useful signature peptides (NSQVWLGR and HNLFEPEDTGQR) of human kallikrein-2 (hK2 - NX_P20151) and developed an SRM assay. Details of the analytical parameters, including multiple transitions by peptides, are presented. The endogenous levels of hK2 were determined in clinical samples (n = 35). The limit of quantification was also estimated by spiking heavy isotope-labeled peptides into seminal plasma samples at various concentrations (LOQ ≈ 29 ng/mL).
authors: Clinical Protein Science & Imaging, Biomedical Center, Department of Measurement Technology and Industrial Electrical Engineering, Lund University , BMC C13, 221 84 Lund, Sweden.
authors: Akos
authors: A
authors: Végvári
authors: Karin
authors: K
authors: Sjödin
authors: Melinda
authors: M
authors: Rezeli
authors: György
authors: G
authors: Marko-Varga
mesh_terms: D016022:Case-Control Studies; D006801:Humans; D007248:Infertility, Male / Q000378:metabolism; D057230:Limit of Detection; D008297:Male; D012661:Semen / Q000378:metabolism*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods*; D020840:Tissue Kallikreins / Q000378:metabolism*
chemical_list: D020840:Tissue Kallikreins