title: Evaluation and comparison of interleukin-8 (IL-8) level in gingival crevicular fluid in health and severity of periodontal disease: a clinico-biochemical study.
abstract: BACKGROUND
  Cytokines play an important role in the pathology associated with chronic inflammatory diseases. Because of pro-inflammatory and neutrophil chemotactic properties, the cytokines like interleukins (IL) may play a significant role in the pathogenesis of periodontitis. The biological effects of IL-8 are relevant in this regard.
  
  
  OBJECTIVE
  This study was done to compare the level of this molecule in gingival crevicular fluid (GCF) from patients with adult periodontitis (experimental group) and from individuals with clinically healthy gingival (control group).
  
  
  METHODS
  GCF was collected from patients with adult periodontitis and clinically healthy gingival for 30 s using a Periopaper strip and the volume of the sample determined. Following elution of the fluid, assays for IL-8 were carried out by enzyme-linked immunosorbent assay (ELISA). The concentration of IL-8 was calculated in the original volume of GCF on each strip.
  
  
  RESULTS
  The level of IL-8 in the experimental group was significantly higher than in the control group ( P < 0.01). The clinical parameters were positively correlated to IL-8, suggesting that the GCF IL-8 exhibited dynamic changes upon severity of periodontal disease ( P < 0.05).
  
  
  CONCLUSIONS
  These data suggest that level of IL-8 is associated with periodontal status. The level of IL-8 in GCF is valuable in detecting the inflammation of periodontal tissue.
authors: Department of Periodontics, Rural Dental College, Loni, Rahata, Ahmednagar, Maharashtra, India.
authors: Sushma S
authors: SS
authors: Lagdive
authors: Pramod P
authors: PP
authors: Marawar
authors: Girish
authors: G
authors: Byakod
authors: Sanjay B
authors: SB
authors: Lagdive
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D055113:Chronic Periodontitis / Q000276:immunology; D005260:Female; D005881:Gingiva / Q000276:immunology; D005883:Gingival Crevicular Fluid / Q000276:immunology*; D006801:Humans; D016209:Interleukin-8 / Q000032:analysis*; D008297:Male; D008875:Middle Aged; D017622:Periodontal Attachment Loss / Q000276:immunology; D010512:Periodontal Index; D010518:Periodontitis / Q000145:classification / Q000276:immunology*; D055815:Young Adult
chemical_list: C508606:CXCL8 protein, human; D016209:Interleukin-8