title: Mucosal vaccination against tuberculosis using inert bioparticles.
abstract: Needle-free, mucosal immunization is a highly desirable strategy for vaccination against many pathogens, especially those entering through the respiratory mucosa, such as Mycobacterium tuberculosis. Unfortunately, mucosal vaccination against tuberculosis (TB) is impeded by a lack of suitable adjuvants and/or delivery platforms that could induce a protective immune response in humans. Here, we report on a novel biotechnological approach for mucosal vaccination against TB that overcomes some of the current limitations. This is achieved by coating protective TB antigens onto the surface of inert bacterial spores, which are then delivered to the respiratory tract. Our data showed that mice immunized nasally with coated spores developed humoral and cellular immune responses and multifunctional T cells and, most importantly, presented significantly reduced bacterial loads in their lungs and spleens following pathogenic challenge. We conclude that this new vaccine delivery platform merits further development as a mucosal vaccine for TB and possibly also other respiratory pathogens.
authors: Infection and Immunity Research Centre, St. George's University of London, London, United Kingdom.
authors: Rajko
authors: R
authors: Reljic
authors: Laura
authors: L
authors: Sibley
authors: Jen-Min
authors: JM
authors: Huang
authors: Ilaria
authors: I
authors: Pepponi
authors: Andreas
authors: A
authors: Hoppe
authors: Huynh A
authors: HA
authors: Hong
authors: Simon M
authors: SM
authors: Cutting
mesh_terms: D000281:Administration, Intranasal; D058956:Administration, Mucosal; D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology; D000942:Antigens, Bacterial / Q000008:administration & dosage / Q000276:immunology*; D058491:Bacterial Load; D061505:Cell Surface Display Techniques; D004195:Disease Models, Animal; D004337:Drug Carriers / Q000008:administration & dosage; D005260:Female; D008168:Lung / Q000382:microbiology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009169:Mycobacterium tuberculosis / Q000276:immunology*; D013154:Spleen / Q000382:microbiology; D013171:Spores, Bacterial / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology; D014376:Tuberculosis / Q000276:immunology / Q000517:prevention & control*; D032581:Tuberculosis Vaccines / Q000008:administration & dosage / Q000276:immunology*; D014611:Vaccination / Q000379:methods*
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D004337:Drug Carriers; D032581:Tuberculosis Vaccines
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