title: [Antibodies to synthetic fragment 95-123 of the prion protein protect neurons and astrocytes from beta-amyloid toxicity].
abstract: Toxic effect of beta-amyloid on brain cells during Alzheimer's disease pathology is known to be associated with oxidative stress, intracellular Ca2+ increase in neurons and astrocytes and activation of reactive oxygen species production. Prion protein is involved in beta-amyloid toxicity. Here we investigated an effect of affinity purified antibodies to synthetic fragment 95-123 of the prion protein on beta-amyloid induced cell death, Ca(2+)-signal, reactive oxygen species production and caspase 3 activation. We have shown that antibodies to fragment 95-123 are able to protect neurons and astrocytes from beta-amyloid induced cell death with no effect on the intracellular Ca2+ concentration altered by beta-amyloid treatment. However, the antibodies significantly reduced Abeta induced reactive oxygen species production in astrocytes and decreased caspase 3 activation in neurons and astrocytes. Thus, induction of antibodies to synthetic fragment 95-123 of the prion protein provides a new approach to anti-AD vaccine design.
authors: A V
authors: AV
authors: Kamynina
authors: M P
authors: MP
authors: Filatova
authors: D O
authors: DO
authors: Koroev
authors: A Iu
authors: AIu
authors: Abramov
authors: O M
authors: OM
authors: Vol'pina
mesh_terms: D000544:Alzheimer Disease / Q000276:immunology / Q000628:therapy*; D016229:Amyloid beta-Peptides / Q000276:immunology* / Q000378:metabolism; D000818:Animals; D000906:Antibodies / Q000737:chemistry / Q000494:pharmacology*; D017209:Apoptosis / Q000276:immunology; D001253:Astrocytes / Q000276:immunology / Q000378:metabolism; D002118:Calcium / Q000378:metabolism; D002478:Cells, Cultured; D002540:Cerebral Cortex; D006801:Humans; D007167:Immunotherapy; D009474:Neurons / Q000276:immunology / Q000378:metabolism; D018384:Oxidative Stress; D010446:Peptide Fragments / Q000138:chemical synthesis / Q000276:immunology; D010455:Peptides / Q000138:chemical synthesis / Q000276:immunology*; D011328:Prions / Q000737:chemistry / Q000276:immunology*; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D016229:Amyloid beta-Peptides; D000906:Antibodies; D010446:Peptide Fragments; D010455:Peptides; D011328:Prions; D017382:Reactive Oxygen Species; D002118:Calcium