title: Kazachstania psychrophila sp. nov., a novel psychrophilic yeast isolated from vacuum-packed beef.
abstract: Five novel ascosporogenous yeast strains (H382, H396, H409, H433(T) and H441) were found through a survey of vacuum-packed beef microbiota. Sequence analysis of ITS domain and LSU rRNA genes showed that the new strains represent a distinct lineage within the genus Kazachstania, closely related to Kazachstania lodderae (97.0 % identity) and Kazachstania ichnusensis (96.1 % identity). The main difference of strains H382, H396, H409, H433(T) and H441 to strains of known Kazachstania species is the maximum growth temperature, which is below 20 °C for the new strains, whereas related species grow at 25 °C. Furthermore, the strains differed from known Kazachstania species in assimilation and fermentation patterns of carbon sources. Based on these characteristics, the five strains are considered to represent a novel species of the genus Kazachstania for which the name Kazachstania psychrophila sp. nov. is proposed. The type strain is H433(T) (DSM 26230(T)=CBS 12689(T)). The Mycobank number of the type strain is MB 803980.
authors: Department of Microbiology and Biotechnology, Federal Research Institute of Nutrition and Food, Max Rubner-Institute, Hermann-Weigmannstraße 1, 24103, Kiel, Germany, jan.kabisch@mri.bund.de.
authors: Jan
authors: J
authors: Kabisch
authors: Constanze
authors: C
authors: Höning
authors: Christina
authors: C
authors: Böhnlein
authors: Rohtraud
authors: R
authors: Pichner
authors: Manfred
authors: M
authors: Gareis
authors: Mareike
authors: M
authors: Wenning
mesh_terms: D002244:Carbon / Q000378:metabolism; D016000:Cluster Analysis; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics; D005285:Fermentation; D005516:Food Microbiology; D020459:Genes, rRNA; D008853:Microscopy; D008969:Molecular Sequence Data; D016533:Mycological Typing Techniques; D010802:Phylogeny; D012331:RNA, Fungal / Q000235:genetics; D012335:RNA, Ribosomal / Q000235:genetics; D004718:Saccharomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D017422:Sequence Analysis, DNA; D013696:Temperature
chemical_list: D004271:DNA, Fungal; D004275:DNA, Ribosomal; D021903:DNA, Ribosomal Spacer; D012331:RNA, Fungal; D012335:RNA, Ribosomal; C065096:RNA, ribosomal, 26S; D002244:Carbon