title: Enhancing the cellulose-degrading activity of cellulolytic bacteria CTL-6 (Clostridium thermocellum) by co-culture with non-cellulolytic bacteria W2-10 (Geobacillus sp.).
abstract: The effect of a non-cellulolytic bacterium W2-10 (Geobacillus sp.) on the cellulose-degrading activity of a cellulolytic bacterium CTL-6 (Clostridium thermocellum) was determined using cellulose materials (paper and straw) in peptone cellulose solution (PCS) medium under aerobic conditions. The results indicated that in the co-culture, addition of W2-10 resulted in a balanced medium pH, and may provide the required anaerobic environment for CTL-6. Overall, addition of W2-10 was beneficial to CTL-6 growth in the adverse environment of the PCS medium. In co-culture with W2-10, the CTL-6 cellulose degradation efficiency of filter paper and alkaline-treated wheat straw significantly increased up to 72.45 and 37.79 %, respectively. The CMCase activity and biomass of CTL-6 also increased from 0.23 U ml(-1) and 45.1 μg ml(-1) (DNA content) up to 0.47 U ml(-1) and 112.2 μg ml(-1), respectively. In addition, co-culture resulted in accumulation of acetate and propionate up to 4.26 and 2.76 mg ml(-1). This was a respective increase of 2.58 and 4.45 times, in comparison to the monoculture with CTL-6.
authors: College of Chemistry and Life Science/Research Institute for New Energy, China Three Gorges University, Yichang, 443002, China.
authors: Yucai
authors: Y
authors: Lü
authors: Ning
authors: N
authors: Li
authors: Xufeng
authors: X
authors: Yuan
authors: Binbin
authors: B
authors: Hua
authors: Jungang
authors: J
authors: Wang
authors: Masaharu
authors: M
authors: Ishii
authors: Yasuo
authors: Y
authors: Igarashi
authors: Zongjun
authors: Z
authors: Cui
mesh_terms: D018533:Biomass; D002480:Cellulase / Q000378:metabolism; D002482:Cellulose / Q000378:metabolism*; D048013:Acetivibrio thermocellus / Q000201:enzymology / Q000254:growth & development* / Q000378:metabolism*; D018920:Coculture Techniques; D003470:Culture Media / Q000737:chemistry; D056506:Geobacillus / Q000254:growth & development*; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis
chemical_list: D003470:Culture Media; D002482:Cellulose; D002480:Cellulase; C019826:carboxymethylcellulase