title: Monocyte chemoattractant protein-1 deficiency attenuates oxidative stress and protects against ovariectomy-induced chronic inflammation in mice.
abstract: BACKGROUND
  Loss of ovarian function is highly associated with an elevated risk of metabolic disease. Monocyte chemoattractant protein-1 (MCP-1, C-C chemokine ligand 2) plays critical roles in the development of inflammation, but its role in ovariectomy (OVX)-induced metabolic disturbance has not been known.
  
  
  RESULTS
  We investigated the role of MCP-1 in OVX-induced metabolic perturbation using MCP-1-knockout mice. OVX increased fat mass, serum levels of MCP-1, macrophage-colony stimulating factor (M-CSF), and reactive oxygen species (ROS), whereas MCP-1 deficiency attenuated these. OVX-induced increases of visceral fat resulted in elevated levels of highly inflammatory CD11c-expressing cells as well as other immune cells in adipose tissue, whereas a lack of MCP-1 significantly reduced all of these levels. MCP-1 deficiency attenuated activation of phospholipase Cγ2, transforming oncogene from Ak strain, and extracellular signal-regulated kinase as well as generation of ROS, which is required for up-regulating CD11c expression upon M-CSF stimulation in bone marrow-derived macrophages.
  
  
  CONCLUSIONS
  Our data suggested that MCP-1 plays a key role in developing metabolic perturbation caused by a loss of ovarian functions through elevating CD11c expression via ROS generation.
authors: Department of Biological Sciences, University of Ulsan, Ulsan, Korea.
authors: Woon-Ki
authors: WK
authors: Kim
authors: Eun-Kyung
authors: EK
authors: Choi
authors: Ok-Joo
authors: OJ
authors: Sul
authors: Yeon-Kyung
authors: YK
authors: Park
authors: Eun-Sook
authors: ES
authors: Kim
authors: Rina
authors: R
authors: Yu
authors: Jae-Hee
authors: JH
authors: Suh
authors: Hye-Seon
authors: HS
authors: Choi
mesh_terms: D050154:Adiposity; D000818:Animals; D039521:CD11c Antigen / Q000378:metabolism; D018932:Chemokine CCL2 / Q000172:deficiency* / Q000235:genetics; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D005260:Female; D007249:Inflammation / Q000378:metabolism; D007333:Insulin Resistance; D050152:Intra-Abdominal Fat / Q000473:pathology; D008262:Macrophage Activation; D016173:Macrophage Colony-Stimulating Factor / Q000502:physiology; D008264:Macrophages / Q000276:immunology / Q000378:metabolism; D024821:Metabolic Syndrome / Q000276:immunology / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D010052:Ovariectomy; D018384:Oxidative Stress; D051966:Phospholipase C gamma / Q000378:metabolism; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D050151:Subcutaneous Fat / Q000473:pathology
chemical_list: D039521:CD11c Antigen; C491768:Ccl2 protein, mouse; D018932:Chemokine CCL2; D017382:Reactive Oxygen Species; D016173:Macrophage Colony-Stimulating Factor; D051057:Proto-Oncogene Proteins c-akt; D048049:Extracellular Signal-Regulated MAP Kinases; D051966:Phospholipase C gamma
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