title: A new tool improves diagnostic test performance for transmission em evaluation of axonemal dynein arms.
abstract: Abstract Diagnosis of primary ciliary dyskinesia (PCD) by identification of dynein arm loss in transmission electron microscopy (TEM) images can be confounded by high background noise due to random electron-dense material within the ciliary matrix, leading to diagnostic uncertainty even for experienced morphologists. The authors developed a novel image analysis tool to average the axonemal peripheral microtubular doublets, thereby increasing microtubular signal and reducing random background noise. In a randomized, double-blinded study that compared two experienced morphologists and three different diagnostic approaches, they found that use of this tool led to improvement in diagnostic TEM test performance.
authors: Department of Biostatistics .
authors: W Keith
authors: WK
authors: Funkhouser
authors: Marc
authors: M
authors: Niethammer
authors: Johnny L
authors: JL
authors: Carson
authors: Kimberlie A
authors: KA
authors: Burns
authors: Michael R
authors: MR
authors: Knowles
authors: Margaret W
authors: MW
authors: Leigh
authors: Maimoona A
authors: MA
authors: Zariwala
authors: William K
authors: WK
authors: Funkhouser
mesh_terms: D056444:Axonemal Dyneins / Q000648:ultrastructure*; D004311:Double-Blind Method; D006801:Humans; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D007619:Kartagener Syndrome / Q000175:diagnosis*; D046529:Microscopy, Electron, Transmission / Q000379:methods*; D015203:Reproducibility of Results
chemical_list: D056444:Axonemal Dyneins
keywords: PCD; Primary ciliary dyskinesia; dynein arm; image analysis
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