title: Blm10 facilitates nuclear import of proteasome core particles.
abstract: Short-lived proteins are degraded by proteasome complexes, which contain a proteolytic core particle (CP) but differ in the number of regulatory particles (RPs) and activators. A recently described member of conserved proteasome activators is Blm10. Blm10 contains 32 HEAT-like modules and is structurally related to the nuclear import receptor importin/karyopherin β. In proliferating yeast, RP-CP assemblies are primarily nuclear and promote cell division. During quiescence, RP-CP assemblies dissociate and CP and RP are sequestered into motile cytosolic proteasome storage granuli (PSG). Here, we show that CP sequestration into PSG depends on Blm10, whereas RP sequestration into PSG is independent of Blm10. PSG rapidly clear upon the resumption of cell proliferation and proteasomes are relocated into the nucleus. Thereby, Blm10 facilitates nuclear import of CP. Blm10-bound CP serves as an import receptor-cargo complex, as Blm10 mediates the interaction with FG-rich nucleoporins and is dissociated from the CP by Ran-GTP. Thus, Blm10 represents the first CP-dedicated nuclear import receptor in yeast.
authors: 1] Department of Biochemistry, University of Toronto, One King's College Circle, Toronto, Ontario, Canada [2] Institute of Biochemistry, University of Stuttgart, Stuttgart, Germany.
authors: Marion H
authors: MH
authors: Weberruss
authors: Anca F
authors: AF
authors: Savulescu
authors: Julia
authors: J
authors: Jando
authors: Thomas
authors: T
authors: Bissinger
authors: Amnon
authors: A
authors: Harel
authors: Michael H
authors: MH
authors: Glickman
authors: Cordula
authors: C
authors: Enenkel
mesh_terms: D021581:Active Transport, Cell Nucleus / Q000235:genetics; D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism*; D049109:Cell Proliferation; D003594:Cytoplasmic Granules / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D008954:Models, Biological; D028861:Nuclear Pore Complex Proteins / Q000378:metabolism; D030781:Organisms, Genetically Modified; D046988:Proteasome Endopeptidase Complex / Q000737:chemistry / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D018160:Receptors, Cytoplasmic and Nuclear / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D014981:Xenopus
chemical_list: C500597:Blm10 protein, S cerevisiae; C116843:NUP53 protein, S cerevisiae; D028861:Nuclear Pore Complex Proteins; D018160:Receptors, Cytoplasmic and Nuclear; D029701:Saccharomyces cerevisiae Proteins; D049452:Green Fluorescent Proteins; D046988:Proteasome Endopeptidase Complex
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