title: Dietary fat in relation to erythrocyte fatty acid composition in men.
abstract: Erythrocyte membrane fatty acid (EMFA) composition is used in the validation of food frequency questionnaires (FFQ) and the evaluation of dietary fat quality. In this cross-sectional study we aimed to investigate associations of diet with EMFA. Altogether, 1,033 randomly selected Finnish men, aged from 47 to 75 years filled in a FFQ and their EMFA composition was analyzed. Marine polyunsaturated fatty acid (PUFA) intake correlated positively with erythrocyte eicosapentaenoic and docosahexaenoic acids (r(s) = 0.415 and r(s) = 0.340, respectively, P < 0.001) and inversely with all n-6 PUFA analyzed (P < 0.001). PUFA intake from spreads and cooking fats correlated positively with alpha-linolenic (ALA), linoleic (LNA) and nervonic acids (r(s) = 0.229, r(s) = 0.160 and r(s) = 0.143, respectively, P < 0.001). Milk fat intake was associated with myristic and behenic acids (r(s) = 0.186 and r(s) = 0.132, respectively P < 0.001). Butter users had lower ALA and LNA proportions (mol%) than non-users (0.16 ± 0.04 vs. 0.19 ± 0.05, P < 0.001 and 7.77 ± 1.02 vs. 8.12 ± 1.11, P = 0.001). Higher PUFA intake from meat was related to decreased long-chain n-3 (P < 0.001) and increased n-6 PUFA (P < 0.001) proportions. In conclusion, EMFA composition reflects particularly well the intakes of n-3 PUFA, whereas other associations remained lower. Yet, all main sources of dietary fat were related with EMFA. The dietary effect on the nervonic acid proportion was confirmed.
authors: Institute of Public Health and Clinical Nutrition, University of Eastern Finland, P.O. Box 1627, 70211, Kuopio, Finland, markust@student.uef.fi.
authors: Markus
authors: M
authors: Takkunen
authors: Jyrki
authors: J
authors: Agren
authors: Johanna
authors: J
authors: Kuusisto
authors: Markku
authors: M
authors: Laakso
authors: Matti
authors: M
authors: Uusitupa
authors: Ursula
authors: U
authors: Schwab
mesh_terms: D000368:Aged; D002462:Cell Membrane / Q000378:metabolism*; D003430:Cross-Sectional Studies; D004032:Diet; D004281:Docosahexaenoic Acids / Q000378:metabolism*; D015118:Eicosapentaenoic Acid / Q000378:metabolism*; D004912:Erythrocytes / Q000378:metabolism*; D005227:Fatty Acids / Q000378:metabolism; D006801:Humans; D008297:Male; D024821:Metabolic Syndrome / Q000097:blood; D008875:Middle Aged; D011795:Surveys and Questionnaires
chemical_list: D005227:Fatty Acids; D004281:Docosahexaenoic Acids; D015118:Eicosapentaenoic Acid
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