title: Fine spatiotemporal control of nitric oxide release by infrared pulse-laser irradiation of a photolabile donor.
abstract: Two-photon-excitation release of nitric oxide (NO) from our recently synthesized photolabile NO donor, Flu-DNB, was confirmed to allow fine spatial and temporal control of NO release at the subcellular level in vitro. We then evaluated in vivo applications. Femtosecond near-infrared pulse laser irradiation of predefined regions of interest in living mouse brain treated with Flu-DNB induced NO-release-dependent, transient vasodilation specifically at the irradiated site. Photoirradiation in the absence of Flu-DNB had no effect. Further, NO release from Flu-DNB by pulse laser irradiation was shown to cause chemoattraction of microglial processes to the irradiated area in living mouse brain. To our knowledge, this is the first demonstration of induction of biological responses in vitro and in vivo by means of precisely controlled, two-photon-mediated release of NO.
authors: Graduate School of Pharmaceutical Sciences, Nagoya City University , 3-1, Tanabe-dori, Mizuho-ku, Nagoya, Aichi 467-8603, Japan.
authors: Hidehiko
authors: H
authors: Nakagawa
authors: Kazuhiro
authors: K
authors: Hishikawa
authors: Kei
authors: K
authors: Eto
authors: Naoya
authors: N
authors: Ieda
authors: Tomotaka
authors: T
authors: Namikawa
authors: Kenji
authors: K
authors: Kamada
authors: Takayoshi
authors: T
authors: Suzuki
authors: Naoki
authors: N
authors: Miyata
authors: Jun-ichi
authors: J
authors: Nabekura
mesh_terms: D000818:Animals; D001921:Brain / Q000187:drug effects; D045744:Cell Line, Tumor; D005452:Fluoresceins / Q000737:chemistry*; D006801:Humans; D007259:Infrared Rays; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018613:Microscopy, Confocal; D009569:Nitric Oxide / Q000737:chemistry* / Q000378:metabolism; D009578:Nitrobenzenes / Q000737:chemistry*
chemical_list: C000595255:Flu-DNB; D005452:Fluoresceins; D009578:Nitrobenzenes; D009569:Nitric Oxide