title: Centrosomal protein CEP104 (Chlamydomonas FAP256) moves to the ciliary tip during ciliary assembly.
abstract: The ciliary tip has been implicated in ciliary assembly and disassembly, and signaling, yet information on its protein composition is limited. Using comparative, quantitative proteomics based on the fact that tip proteins will be approximately twice as concentrated in half-length compared with full-length flagella, we have identified FAP256 as a tip protein in Chlamydomonas. FAP256 localizes to the tips of both central pair and outer doublet microtubules (MTs) and it remains at the tip during flagellar assembly and disassembly. Similarly, its vertebrate counterpart, CEP104, localizes on the distal ends of both centrioles of nondividing cells until the mother centriole forms a cilium and then localizes at the tip of the elongating cilium. A null mutant of FAP256 in Chlamydomonas and RNAi in vertebrate cells showed that FAP256/CEP104 is required for ciliogenesis in a high percentage of cells. In those cells that could form cilia, there were structural deformities at the ciliary tips.
authors: Department of Molecular Cellular and Developmental Biology, Yale University, New Haven, CT 06520, USA.
authors: Trinadh V
authors: TV
authors: Satish Tammana
authors: Damayanti
authors: D
authors: Tammana
authors: Dennis R
authors: DR
authors: Diener
authors: Joel
authors: J
authors: Rosenbaum
mesh_terms: D002455:Cell Division; D018385:Centrosome / Q000378:metabolism*; D016825:Chlamydomonas reinhardtii / Q000166:cytology / Q000235:genetics / Q000378:metabolism*; D002923:Cilia / Q000235:genetics / Q000378:metabolism*; D005407:Flagella / Q000235:genetics / Q000378:metabolism*; D008870:Microtubules / Q000235:genetics / Q000378:metabolism; D021381:Protein Transport
keywords: CEP104; Centrosomal proteins; Ciliary tip; Ciliogenesis; FAP256; Flagellar assembly/disassembly
references: Curr Biol. 2005 Feb 8;15(3):262-6
references: 15694311
references: Eukaryot Cell. 2007 Dec;6(12):2354-64
references: 17766466
references: Mol Biochem Parasitol. 2010 Dec;174(2):137-40
references: 20728476
references: J Cell Biol. 1978 Jul;78(1):8-27
references: 149796
references: Mol Biol Cell. 2006 Sep;17(9):3781-92
references: 16775004
references: Curr Biol. 2003 Nov 11;13(22):1969-74
references: 14614822
references: Science. 1989 Dec 15;246(4936):1503-4
references: 17756009
references: J Cell Biol. 1972 Sep;54(3):507-39
references: 4558009
references: Cell. 2004 May 14;117(4):541-52
references: 15137946
references: Dev Cell. 2012 Dec 11;23(6):1167-75
references: 23168168
references: J Cell Sci. 1968 Jun;3(2):207-30
references: 5661997
references: J Cell Biol. 1967 Jul;34(1):345-64
references: 6033540
references: J Cell Biol. 2013 Feb 18;200(4):505-22
references: 23400999
references: J Cell Biol. 1969 May;41(2):600-19
references: 5783876
references: Curr Biol. 2007 May 1;17(9):778-82
references: 17433682
references: J Cell Biol. 1977 Sep;74(3):747-59
references: 903371
references: Curr Opin Cell Biol. 2005 Feb;17(1):82-8
references: 15661523
references: Proc Natl Acad Sci U S A. 1993 Jun 15;90(12):5519-23
references: 8516294
references: J Cell Biol. 2010 Oct 18;191(2):415-28
references: 20956384
references: J Ultrastruct Res. 1979 Aug;68(2):173-85
references: 480410
references: Ann N Y Acad Sci. 1953 Oct 14;56(5):1091-3
references: 13139305
references: J Cell Biol. 1998 May 18;141(4):993-1008
references: 9585417
references: Proc Natl Acad Sci U S A. 2009 Aug 11;106(32):13377-82
references: 19666503
references: J Cell Biol. 1967 Jun;33(3):543-71
references: 5341020
references: Genes Dev. 2008 Apr 1;22(7):918-30
references: 18334618
references: EMBO J. 2011 Apr 20;30(8):1520-35
references: 21399614
references: J Cell Sci. 2011 Aug 1;124(Pt 15):2539-51
references: 21768326
references: Cell Motil Cytoskeleton. 2006 Dec;63(12):741-6
references: 17009329
references: Proc Natl Acad Sci U S A. 2009 Mar 24;106(12):4713-8
references: 19264963
references: Cell. 2012 Nov 9;151(4):847-858
references: 23141541
references: J Cell Biol. 1992 Dec;119(6):1605-11
references: 1281816
references: Methods Enzymol. 2013;524:105-22
references: 23498737
references: Curr Biol. 2012 Oct 9;22(19):1800-7
references: 22885064
references: Ann N Y Acad Sci. 1975 Jun 30;253:178-91
references: 1056740
references: Curr Biol. 2006 Mar 7;16(5):450-9
references: 16527740
references: Mol Biol Cell. 2005 Jan;16(1):270-8
references: 15496456
references: J Cell Biol. 2005 Jul 4;170(1):103-13
references: 15998802
references: Cell. 2011 Jun 10;145(6):914-25
references: 21620453
references: Bull Eur Physiopathol Respir. 1986 Jan-Feb;22(1):43-7
references: 3697548
references: Curr Biol. 2012 Mar 6;22(5):414-9
references: 22326026
references: Mol Cell. 2003 Feb;11(2):445-57
references: 12620232
references: J Cell Biol. 1990 Mar;110(3):703-14
references: 2106524
references: Methods Cell Biol. 2009;94:333-46
references: 20362099
references: PLoS Genet. 2005 Oct;1(4):e53
references: 16254602
references: J Cell Sci. 1974 Dec;16(3):529-56
references: 4615103
references: Cytobios. 1974 Mar-Apr;9(35):142-61
references: 4603233
references: J Cell Sci. 1980 Apr;42:207-20
references: 6772653
references: J Cell Biol. 2012 Jul 9;198(1):37-45
references: 22778277
references: Mol Biol Cell. 2008 Oct;19(10):4319-27
references: 18701702
references: Mol Cell Proteomics. 2004 Dec;3(12):1154-69
references: 15385600
references: Methods Enzymol. 2013;524:243-63
references: 23498744
references: J Cell Biol. 1968 Aug;38(2):403-25
references: 5664210
references: Trends Cell Biol. 2008 Jan;18(1):8-11
references: 18068367
references: Nat Rev Genet. 2010 May;11(5):331-44
references: 20395968
references: J Cell Biol. 2009 Aug 24;186(4):601-13
references: 19704024
references: Cell. 2007 Aug 24;130(4):678-90
references: 17719545
references: Cell Biol Int. 1996 Jan;20(1):73-81
references: 8936410
references: J Cell Sci. 1998 Feb;111 ( Pt 3):313-20
references: 9427680
references: J Cell Biol. 2009 Oct 5;187(1):135-48
references: 19805633
references: Mol Cell Proteomics. 2002 Jun;1(6):451-65
references: 12169685
references: J Cell Biol. 2001 Oct 29;155(3):405-14
references: 11684707
references: Mol Microbiol. 2008 Nov;70(4):837-52
references: 18793337
references: Mol Biol Cell. 2008 Dec;19(12):5338-46
references: 18829862
references: FASEB J. 2010 Sep;24(9):3117-21
references: 20371625
references: J Cell Biol. 1963 Apr;17:208-12
references: 13986422
references: J Proteome Res. 2011 Aug 5;10(8):3830-9
references: 21663328
references: Cell. 1980 Jun;20(2):469-77
references: 6156007