title: [Cardiomyocyte differentiation of individual clones murine induced pluripotent stem cells].
abstract: Cardiomyocyte differentiation of certain clones of murine induced pluripotent stem cells (iPS) was estimated. iPS were obtained due to reprogramming of murine embryonic fibroblasts with transposon-based Sleeping beauty plasmids as sene delivery systems. Differentiation was performed in suspension culture and in attached to tissue-culture plates embryoid bodies (EBs). Ascorbic acid was applied as inductor. According to the obtained results, the differentiation was tenfold more effective in attached EBs. Ascorbic acid stimulated the generation of cardiomiocytes.
authors: S V
authors: SV
authors: Malysheva
authors: H V
authors: HV
authors: Budash
authors: N M
authors: NM
authors: Bil'ko
authors: J
authors: J
authors: Heschheller
mesh_terms: D000818:Animals; D001205:Ascorbic Acid / Q000494:pharmacology; D002454:Cell Differentiation; D002478:Cells, Cultured; D002999:Clone Cells; D004251:DNA Transposable Elements; D004622:Embryo, Mammalian; D058732:Embryoid Bodies / Q000166:cytology* / Q000187:drug effects / Q000502:physiology; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins; D057026:Induced Pluripotent Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000502:physiology; D051379:Mice; D008822:Mice, Transgenic; D032383:Myocytes, Cardiac / Q000166:cytology* / Q000187:drug effects / Q000502:physiology; D010957:Plasmids; D014162:Transfection
chemical_list: D004251:DNA Transposable Elements; D049452:Green Fluorescent Proteins; D001205:Ascorbic Acid