title: Increased production of cellulases and xylanases by Penicillium echinulatum S1M29 in batch and fed-batch culture.
abstract: The development of more productive strains of microorganisms and processes that increase enzyme levels can contribute to the economically efficient production of second generation ethanol. To this end, cellulases and xylanases were produced with the S1M29 mutant strain of Penicillium echinulatum, using different concentrations of cellulose (20, 40, and 60 g L(-1)) in batch and fed-batch processes. The highest activities of FPase (8.3 U mL(-1)), endoglucanases (37.3 U mL(-1)), and xylanases (177 U mL(-1)) were obtained in fed-batch cultivation with 40 g L(-1) of cellulose. The P. echinulatum enzymatic broth and the commercial enzyme Cellic CTec2 were tested for hydrolysis of pretreated sugar cane bagasse. Maximum concentrations of glucose and xylose were achieved after 72 h of hydrolysis. Glucose yields of 28.0% and 27.0% were obtained using the P. echinulatum enzymatic extract and Cellic CTec2, respectively.
authors: Enzymes and Biomass Laboratory, Institute of Biotechnology, University of Caxias do Sul, Rua Francisco Getúlio Vargas 1130, 95070-560 Caxias do Sul, RS, Brazil.
authors: Laísa
authors: L
authors: Dos Reis
authors: Enzymes and Biomass Laboratory, Institute of Biotechnology, University of Caxias do Sul, Rua Francisco Getúlio Vargas 1130, 95070-560 Caxias do Sul, RS, Brazil.
authors: Roselei Claudete
authors: RC
authors: Fontana
authors: Brazilian Bioethanol Science and Technology Laboratory - CTBE, Rua Giuseppe Maximo Scolfaro 10000, Pólo II de Alta Tecnologia, Caxia Postal 6192, 13083-970 Campinas, São Paulo, Brazil.
authors: Priscila
authors: P
authors: da Silva Delabona
authors: Brazilian Bioethanol Science and Technology Laboratory - CTBE, Rua Giuseppe Maximo Scolfaro 10000, Pólo II de Alta Tecnologia, Caxia Postal 6192, 13083-970 Campinas, São Paulo, Brazil.
authors: Deise Juliana
authors: DJ
authors: da Silva Lima
authors: Enzymes and Biomass Laboratory, Institute of Biotechnology, University of Caxias do Sul, Rua Francisco Getúlio Vargas 1130, 95070-560 Caxias do Sul, RS, Brazil.
authors: Marli
authors: M
authors: Camassola
authors: Brazilian Bioethanol Science and Technology Laboratory - CTBE, Rua Giuseppe Maximo Scolfaro 10000, Pólo II de Alta Tecnologia, Caxia Postal 6192, 13083-970 Campinas, São Paulo, Brazil.
authors: José Geraldo
authors: JG
authors: da Cruz Pradella
authors: Enzymes and Biomass Laboratory, Institute of Biotechnology, University of Caxias do Sul, Rua Francisco Getúlio Vargas 1130, 95070-560 Caxias do Sul, RS, Brazil. Electronic address: ajpdillo@ucs.br.
authors: Aldo José Pinheiro
authors: AJP
authors: Dillon
mesh_terms: D061249:Batch Cell Culture Techniques; D001673:Biodegradation, Environmental; D019149:Bioreactors; D002480:Cellulase / Q000096:biosynthesis*; D002482:Cellulose / Q000737:chemistry*; D043364:Endo-1,4-beta Xylanases / Q000096:biosynthesis*; D000431:Ethanol / Q000737:chemistry; D005285:Fermentation; D005947:Glucose / Q000737:chemistry; D006868:Hydrolysis; D010100:Oxygen / Q000737:chemistry; D010407:Penicillium / Q000378:metabolism*; D031786:Saccharum; D013997:Time Factors; D014994:Xylose / Q000737:chemistry
chemical_list: D000431:Ethanol; D002482:Cellulose; C027433:bagasse; D014994:Xylose; D002480:Cellulase; D043364:Endo-1,4-beta Xylanases; D005947:Glucose; D010100:Oxygen
keywords: Batch; Cellulases; Fed-batch; Penicillium echinulatum; Xylanases