title: Proteomic analysis of cloned porcine conceptuses during the implantation period.
abstract: Differentially regulated proteins within porcine somatic cell nuclear transfer (SCNT)-derived conceptuses were compared with conceptuses that were derived from natural matings on day 14 of pregnancy. Proteins that were expressed prominently on day 14 were identified in SCNT-derived conceptuses using 2-D PAGE and MALDI-TOF MS. Sixty eight proteins were identified as being differentially regulated in the SCNT-derived conceptuses. Among these, 62 were down-regulated whereas the other six proteins were up-regulated. Glycolytic proteins, such as pyruvate dehydrogenase, malate dehydrogenase and lactate dehydrogenase, were down-regulated in the SCNT-derived conceptuses whereas apoptosis-related genes as annexin V, Hsp60, and lamin A were up-regulated. Thus, apoptosis-related genes are expressed at significantly higher levels in the SCNT-derived conceptuses than in the control conceptuses, whereas metabolism-related genes are significantly reduced.
authors: Animal Genetic Resources Station, National Institute of Animal Science, RDA, Namwon, 590-832, Korea, kog4556@korea.kr.
authors: Yeoung-Gyu
authors: YG
authors: Ko
authors: Hae-Geum
authors: HG
authors: Park
authors: Gyu-Tae
authors: GT
authors: Yeom
authors: Seongsoo
authors: S
authors: Hwang
authors: Hyun
authors: H
authors: Kim
authors: Soo-Bong
authors: SB
authors: Park
authors: Bo-Suck
authors: BS
authors: Yang
authors: Young Min
authors: YM
authors: Song
authors: Jae-Hyeon
authors: JH
authors: Cho
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D030801:Animals, Genetically Modified / Q000235:genetics / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D004622:Embryo, Mammalian / Q000737:chemistry / Q000378:metabolism* / Q000473:pathology; D005260:Female; D053652:Nuclear Transfer Techniques; D020543:Proteome / Q000032:analysis / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*; D013552:Swine / Q000235:genetics / Q000378:metabolism
chemical_list: D020543:Proteome