title: Metabolic pathways of T-2 toxin in in vivo and in vitro systems of Wistar rats.
abstract: In the present study, metabolites of T-2 toxin in in vivo and in vitro systems of Wistar rats were identified and elucidated by ultraperformance liquid chromatography-quadrupole/time-of-flight tandem mass spectrometry (UPLC-Q/TOF-MS). Expected and unexpected metabolites were detected by Metabolynx(XS) software, which could automatically compare MS(E) data from the sample and control. A total of 19 metabolites of T-2 toxin were identified in this research, 9 of them being novel, which were 15-deacetyl-T-2, 3'-OH-15-deacetyl-T-2, 3',7-dihydroxy-T-2, isomer of 3',7-dihydroxy-T-2, 7-OH-HT-2, isomer of 7-OH-HT-2, de-epoxy-3',7-dihydroxy-HT-2, 9-OH-T-2, and 3',9-dihydroxy-T-2. The results showed that the main metabolic pathways of T-2 toxin were hydrolysis, hydroxylation, and de-epoxidation. In addition, the results also revealed one novel metabolic pathway of T-2 toxin, hydroxylation at C-9 position, which was demonstrated by the metabolites 9-OH-T-2 and 3',9-dihydroxy-T-2. In addition, hydroxylation at C-9 of T-2 toxin was also generated in in vitro of liver systems. Interestingly, several metabolites of hydroxylation at C-7 of T-2 toxin were also detected in in vivo male Wistar rats, but they were not found in in vivo female rats and in in vitro systems of Wistar rats.
authors: Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, China Agricultural University , Beijing 100193, People's Republic of China.
authors: Shupeng
authors: S
authors: Yang
authors: Yanshen
authors: Y
authors: Li
authors: Xingyuan
authors: X
authors: Cao
authors: Dingfei
authors: D
authors: Hu
authors: Zhanhui
authors: Z
authors: Wang
authors: Ying
authors: Y
authors: Wang
authors: Jianzhong
authors: J
authors: Shen
authors: Suxia
authors: S
authors: Zhang
mesh_terms: D000818:Animals; D002853:Chromatography, Liquid; D005260:Female; D006900:Hydroxylation; D008297:Male; D013058:Mass Spectrometry; D053858:Metabolic Networks and Pathways / Q000502:physiology*; D008862:Microsomes, Liver / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012984:Software; D013605:T-2 Toxin / Q000008:administration & dosage / Q000378:metabolism* / Q000652:urine
chemical_list: D013605:T-2 Toxin