title: Protective Effect of γ-Irradiation Against Hypochlorous Acid-Induced Haemolysis in Human Erythrocytes.
abstract: Radiations may trigger protective response within a threshold of doses applied. Exposures above an upper threshold are generally detrimental, while exposures below a lower threshold may or may not increase risks for health. We recently reported that a cellular protective response occurs in interventional cardiologists to counteract the oxidative damage caused by radiation. Here, we demonstrated in an in vitro model represented by whole blood of healthy donors γ-irradiated with 220-440 mGy, that haemolysis of erythrocytes induced by hypochlorous acid was reduced by 40%. The protection triggered by γ-radiations made erythrocytes more resistant to oxidative insult caused by hypochlorous acid which was induced 3 h after irradiation and involved biochemical changes in the synthesis and turnover of glutathione. Overall, the biochemical remodelling induced by exposure to γ-radiations might contribute to generate new guidelines in professionally exposed workers.
authors: Institute of Food Sciences, National Research Council, 83100, Avellino, Italy.
authors: Idolo
authors: I
authors: Tedesco
authors: Carmela
authors: C
authors: Spagnuolo
authors: Maria
authors: M
authors: Russo
authors: Roberta
authors: R
authors: Iannitti
authors: Annunziata
authors: A
authors: Nappo
authors: Gian Luigi
authors: GL
authors: Russo
keywords: GSH; human erythrocytes; hypochlorous acid; ionizing radiation; reactive oxygen species
references: Toxicol In Vitro. 1996 Jun;10(3):273-81
references: 20650206
references: J Radiol Prot. 2010 Mar;30(1):1-3
references: 20234068
references: Eur Heart J. 2012 Feb;33(3):408-14
references: 21862464
references: Arch Toxicol. 2009 Mar;83(3):227-47
references: 19234688
references: Int J Toxicol. 2008 Sep-Oct;27(5):369-78
references: 19037807
references: New Biol. 1991 Jul;3(7):651-5
references: 1684284
references: J Nutr Biochem. 2001 Sep;12(9):505-511
references: 11834210
references: Dose Response. 2010 Jan 18;8(2):172-91
references: 20585444
references: J Biol Chem. 2010 Jul 30;285(31):23557-67
references: 20498365
references: Eur Heart J. 2012 Feb;33(4):423-4
references: 22439152
references: Biochem J. 1998 Feb 15;330 ( Pt 1):131-8
references: 9461501
references: Hum Exp Toxicol. 2002 Feb;21(2):85-90
references: 12102502
references: Health Phys. 1999 Dec;77(6):713-8
references: 10568551
references: Clin Chim Acta. 2008 Apr;390(1-2):1-11
references: 18243141
references: Curr Opin Pharmacol. 2007 Aug;7(4):355-9
references: 17602868
references: J Radiat Res. 2007 Mar;48(2):97-106
references: 17327685
references: Dose Response. 2006 Aug 19;4(2):75-90
references: 18648638
references: Dose Response. 2009 Nov 23;8(2):192-208
references: 20585438
references: Radiat Environ Biophys. 2007 Mar;46(1):1-12
references: 17131131
references: Bioelectrochemistry. 2002 Dec;58(2):157-61
references: 12414321
references: Cell Mol Biol Lett. 2003;8(1):215-9
references: 12655375
references: Ann Agric Environ Med. 2008;15(1):9-12
references: 18581973
references: Int J Radiat Biol. 1994 Jan;65(1):27-33
references: 7905906
references: J Nutr Biochem. 2000 Feb;11(2):114-9
references: 10715597
references: FASEB J. 2005 Jun;19(8):998-9
references: 15802491