title: Aligned SF/P(LLA-CL)-blended nanofibers encapsulating nerve growth factor for peripheral nerve regeneration.
abstract: Artificial nerve guidance conduits (NGCs) containing bioactive neurotrophic factors and topographical structure to biomimic native tissues are essential for efficient regeneration of nerve gaps. In this study, aligned SF/P(LLA-CL) nanofibers encapsulating nerve growth factor (NGF), which was stabilized by SF in core, were fabricated via a coaxial electrospinning technique. The controlled release of NGF from the nanofibers was evaluated using enzyme-linked immune sorbent assay (ELISA) and PC12 cell-based bioassay over a 60-day time period. The results demonstrated that NGF presented a sustained release and remained biological activity over 60 days. Nerve guidance conduits (NGCs) were fabricated by reeling the aligned SF/P(LLA-CL) nanofibrous scaffolds encapsulating NGF and then used as a bridge implanted across a 15-mm defect in the sciatic nerve of rats to promote nerve regeneration. The outcome in terms of regenerated nerve at 12 weeks was evaluated by a combination of electrophysiological assessment, histochemistry, and electron microscopy. All results clarified that the NGF-encapsulated-aligned SF/P(LLA-CL) NGCs promoted peripheral nerve regeneration significantly better than the aligned SF/P(LLA-CL) NGCs, suggesting that the released NGF from nanofibers could effectively promote the regeneration of peripheral nerve.
authors: Department of Polymer Materials and Engineering, College of Materials and Textile Engineering, Jiaxing University, Zhejiang, 314001, China.
authors: Zhang
authors: Z
authors: Kuihua
authors: Wang
authors: W
authors: Chunyang
authors: Fan
authors: F
authors: Cunyi
authors: Mo
authors: M
authors: Xiumei
mesh_terms: D000818:Animals; D055724:Electrophysiological Phenomena / Q000187:drug effects; D005349:Fibroins / Q000737:chemistry*; D008297:Male; D057139:Nanofibers / Q000737:chemistry* / Q000648:ultrastructure; D020932:Nerve Growth Factor / Q000494:pharmacology*; D009416:Nerve Regeneration / Q000187:drug effects*; D016716:PC12 Cells; D011091:Polyesters / Q000737:chemistry*; D019919:Prosthesis Implantation; D051381:Rats; D017207:Rats, Sprague-Dawley; D012584:Sciatic Nerve / Q000187:drug effects* / Q000502:physiology* / Q000601:surgery / Q000648:ultrastructure
chemical_list: D011091:Polyesters; C550605:poly(lactic acid-co-epsilon-caprolactone); D005349:Fibroins; D020932:Nerve Growth Factor
keywords: aligned nanofibers; electrospinning; nerve growth factor; peripheral nerve regeneration