title: The role of Sep (O-phosphoserine) tRNA: Sec (selenocysteine) synthase (SEPSECS) in proliferation, apoptosis and hormone secretion of trophoblast cells.
abstract: OBJECTIVE
  To investigate whether Sep (O-phosphoserine) tRNA: Sec (selenocysteine) synthase (SEPSECS), which plays an essential role in the synthesis of selenoprotein, affects proliferation, apoptosis and hormone secretion of human trophoblast cells.
  
  
  METHODS
  Human trophoblast JEG-3 cells were divided into four groups: control group, SEPSECS silenced-expression group, empty vector group and SEPSECS over-expression group. Over-expression and silenced-expression were achieved by transfection with plasmid DNA or RNA oligonucleotide, respectively. 3-[4,5-dimethylthiazol-2-yl] -2,5-diphenyltetrazolium bromide (MTT) and colony formation assays were performed to investigate cell proliferation, while apoptosis was tested by annexin V-FITC, PI double staining and caspases-3 activation assays, enzyme-linked immunosorbent assay (ELISA) was used to determine the level of progesterone (PG) and human chorionic gonadotropin (hCG).
  
  
  RESULTS
  SEPSECS silenced-expression clearly inhibited proliferation of JEG-3 cells (p < 0.05), significantly induced cell apoptosis (p < 0.01) and reduced the production of PG and hCG (p < 0.05). On the contrary, SEPSECS over-expression significantly promoted both cell proliferation (p < 0.01) and secretion of PG and hCG (p < 0.05).
  
  
  CONCLUSIONS
  SEPSECS significantly affects proliferation, apoptosis and hormone secretion of human trophoblast cells, suggesting that a potential relationship exists among SEPSECS, cell proliferation, apoptosis and hormone production of human placental trophoblast cells. Furthermore, this may provide a clue to uncover the relationship between selenium and human placental in association with an emphasis on the importance of selenium adequacy during pregnancy.
authors: Department of Biochemistry and Molecular Biology, Basic Medical Science College, Harbin Medical University, 194 Xuefu Road, Harbin 150081, China.
authors: H-D
authors: HD
authors: Zhao
authors: W-G
authors: WG
authors: Zhang
authors: M-N
authors: MN
authors: Sun
authors: Q-F
authors: QF
authors: Duan
authors: F-L
authors: FL
authors: Li
authors: H
authors: H
authors: Li
mesh_terms: D000604:Amino Acyl-tRNA Synthetases / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D017209:Apoptosis; D002460:Cell Line; D049109:Cell Proliferation; D006063:Chorionic Gonadotropin / Q000378:metabolism; D003114:Colony-Forming Units Assay; D015536:Down-Regulation; D005260:Female; D020868:Gene Silencing; D006801:Humans; D008954:Models, Biological; D010920:Placenta / Q000201:enzymology / Q000378:metabolism*; D010926:Placental Hormones / Q000378:metabolism*; D010929:Placentation; D011247:Pregnancy; D011374:Progesterone / Q000378:metabolism; D034741:RNA, Small Interfering; D012346:RNA, Transfer, Amino Acyl / Q000378:metabolism*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000378:metabolism; D014327:Trophoblasts / Q000201:enzymology / Q000378:metabolism; D015854:Up-Regulation
chemical_list: D006063:Chorionic Gonadotropin; D010926:Placental Hormones; D034741:RNA, Small Interfering; D012346:RNA, Transfer, Amino Acyl; D011994:Recombinant Proteins; C043354:tRNA, O(beta)-phosphoserine-; D011374:Progesterone; D000604:Amino Acyl-tRNA Synthetases; C542370:O-phosphoseryl-tRNA:selenocysteinyl-tRNA synthase, human
keywords: Apoptosis; PG; Proliferation; SEPSECS; Trophoblast; hCG