title: [miR-126 inhibits colon cancer proliferation and invasion through targeting IRS1, SLC7A5 and TOM1 gene].
abstract: OBJECTIVE
  To explore the expression pattern and function of miR-126 in human colon cancer and the underlying mechanisms.
  
  
  METHODS
  The expression pattern of miR-126 in high-density human colon cancer tissue microarray was analyzed by in situ hybridization. Further more, the biological function of miR-126 in colon cancer in vitro was investigated by establishing a stable miR-126 over-expression cell lines.
  
  
  RESULTS
  The expression of miR-126 was lower in the tumor tissue, especially in metastasis tissue. The down-regulation of miR-126 was more obvious in the patients who displayed bad prognosis (P=0.025). Over-expression of miR-126 in colon cancer cell was able to inhibit cell proliferation, promote cell apoptosis and reduce the invasive ability. MiR-126 significantly enhanced the sensitivity of the colon cancer cell to chemotherapeutic drug. It has been shown that IRS1, SLC75A and TOM1 were the potential target genes of miR-126 in colon cancer.
  
  
  CONCLUSIONS
  MiR-126 was able to inhibit the development of colon cancer and its level was closely related with the prognosis of patients with colon cancer. The potential target genes for miR-126 might include IRS1, SLC7A5 and TOM1. Therefore, miR-126 might be a therapeutic target for colon cancer diagnosis and treatment.
authors: Department of Gastroenterology, Third Xiangya Hospital, Central South University, Changsha 410013,China.
authors: Nan
authors: N
authors: Li
authors: Xiayu
authors: X
authors: Li
authors: Shuo
authors: S
authors: Huang
authors: Shourong
authors: S
authors: Shen
authors: Xiaoyan
authors: X
authors: Wang
mesh_terms: D000368:Aged; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D003110:Colonic Neoplasms / Q000235:genetics* / Q000378:metabolism; D005260:Female; D018390:Gene Targeting; D006801:Humans; D055504:Insulin Receptor Substrate Proteins / Q000235:genetics*; D047908:Intracellular Signaling Peptides and Proteins; D027283:Large Neutral Amino Acid-Transporter 1 / Q000235:genetics; D016086:Lentivirus / Q000235:genetics / Q000378:metabolism; D008297:Male; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D011506:Proteins / Q000235:genetics; D014162:Transfection; D059016:Tumor Microenvironment / Q000235:genetics
chemical_list: C526292:IRS1 protein, human; D055504:Insulin Receptor Substrate Proteins; D047908:Intracellular Signaling Peptides and Proteins; D027283:Large Neutral Amino Acid-Transporter 1; C527111:MIRN126 microRNA, human; D035683:MicroRNAs; D011506:Proteins; C120296:TOM1 protein, human