title: Relative prevalence of Mycobacterium marinum in fish collected from aquaria and natural freshwaters in central Europe.
abstract: A survey was carried out on occurrence of Mycobacterium marinum in fish kept in aquaria and those living in their natural environment. Species-specific qPCR targeting the erp and IS2404 genes together with the conventional culture method were used. The analysis of 72 ornamental fish (n = 216 samples: gills, muscle and intestine) collected from aquaria revealed the presence of M. marinum in 30 individuals (41.7%) of whom 17 (23.6%) were later culture positive. Culture-independent detection revealed the presence of M. marinum in 16 of 83 environmental samples (19.3%) collected in aquaria. The presence of viable M. marinum cells was later confirmed in 5 samples (6.0%). No qPCR or culture positivity was observed when 123 groundwater fish and their corresponding environmental samples (n = 142) were analysed.
authors: Veterinary Research Institute, Mycobacteriology Unit, O.I.E. Reference laboratories for Paratuberculosis and Avian Tuberculosis, Brno, Czech Republic.
authors: M
authors: M
authors: Slany
authors: J
authors: J
authors: Makovcova
authors: P
authors: P
authors: Jezek
authors: M
authors: M
authors: Bodnarova
authors: I
authors: I
authors: Pavlik
mesh_terms: D000818:Animals; D000836:Animals, Zoo / Q000382:microbiology*; D001426:Bacterial Proteins / Q000235:genetics; D015169:Colony Count, Microbial / Q000662:veterinary; D018153:Czech Republic / Q000453:epidemiology; D005060:Europe; D005393:Fish Diseases / Q000453:epidemiology* / Q000382:microbiology; D005399:Fishes; D005618:Fresh Water / Q000382:microbiology*; D005880:Gills / Q000382:microbiology; D007422:Intestines / Q000382:microbiology; D008969:Molecular Sequence Data; D060885:Multiplex Polymerase Chain Reaction / Q000662:veterinary; D018482:Muscle, Skeletal / Q000382:microbiology; D009165:Mycobacterium Infections, Nontuberculous / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D019910:Mycobacterium marinum / Q000235:genetics / Q000302:isolation & purification*; D015995:Prevalence; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA / Q000662:veterinary
chemical_list: D001426:Bacterial Proteins; D012336:RNA, Ribosomal, 16S
keywords: ecology; fish mycobacteriosis; molecular diagnostics