title: Cell-based arrays for the identification of interacting polypeptide domains or epitopes.
abstract: The specific regions on proteins which are responsible for protein-protein interaction are called interacting domains, or epitopes in case of antigen-antibody binding. These domains are one feature to characterize proteins and are important in clinical diagnostics and research. For the mapping of such domains the use of protein/peptide arrays has become popular. Regardless of which kind of array, the major requirements are a high number of candidates arranged in the array, high quality, ease of use, and cost-effectiveness. Here, the authors describe a general protocol for mapping the interacting domains of proteins demonstrated by a high affinity protein interaction, the interaction of an antibody to an antigen. The chapter describes a stepwise protocol from library production to the verification of the domain by the use of an automated cell-based polypeptide array, which comprises the named requirements of a good array.
authors: Division of Molecular Dermatology, Department of Dermatology, Paracelsus Private Medical University, Salzburg, Austria.
authors: Richard H
authors: RH
authors: Maier
authors: Christina J
authors: CJ
authors: Maier
authors: Kamil
authors: K
authors: Onder
mesh_terms: D000818:Animals; D000939:Epitopes / Q000032:analysis* / Q000276:immunology; D006801:Humans; D010455:Peptides / Q000032:analysis* / Q000276:immunology; D040081:Protein Array Analysis / Q000379:methods*
chemical_list: D000939:Epitopes; D010455:Peptides