title: Antidiabetic and antioxidant properties of alkaloids from Catharanthus roseus (L.) G. Don.
abstract: Catharanthus roseus (L.) G. Don is a herbal plant traditionally used by local populations in India, South Africa, China and Malaysia to treat diabetes. The present study reports the in vitro antioxidant and antidiabetic activities of the major alkaloids isolated from Catharanthus roseus (L.) G. Don leaves extract. Four alkaloids--vindoline I, vindolidine II, vindolicine III and vindolinine IV--were isolated and identified from the dichloromethane extract (DE) of this plant's leaves. DE and compounds I-III were not cytotoxic towards pancreatic β-TC6 cells at the highest dosage tested (25.0 µg/mL). All four alkaloids induced relatively high glucose uptake in pancreatic β-TC6 or myoblast C2C12 cells, with III showing the highest activity. In addition, compounds II-IV demonstrated good protein tyrosine phosphatase-1B (PTP-1B) inhibition activity, implying their therapeutic potential against type 2 diabetes. III showed the highest antioxidant potential in ORAC and DPPH assays and it also alleviated H₂O₂-induced oxidative damage in β-TC6 cells at 12.5 µg/mL and 25.0 µg/mL.
authors: Department of Chemistry, Faculty of Science, University of Malaya, Kuala Lumpur 50603, Malaysia.
authors: Soon Huat
authors: SH
authors: Tiong
authors: Chung Yeng
authors: CY
authors: Looi
authors: Hazrina
authors: H
authors: Hazni
authors: Aditya
authors: A
authors: Arya
authors: Mohammadjavad
authors: M
authors: Paydar
authors: Won Fen
authors: WF
authors: Wong
authors: Shiau-Chuen
authors: SC
authors: Cheah
authors: Mohd Rais
authors: MR
authors: Mustafa
authors: Khalijah
authors: K
authors: Awang
mesh_terms: D000470:Alkaloids / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D000975:Antioxidants / Q000737:chemistry* / Q000494:pharmacology; D030008:Catharanthus / Q000737:chemistry; D002460:Cell Line; D003920:Diabetes Mellitus / Q000188:drug therapy*; D006801:Humans; D006861:Hydrogen Peroxide / Q000737:chemistry; D007004:Hypoglycemic Agents / Q000737:chemistry / Q000494:pharmacology; D010936:Plant Extracts / Q000737:chemistry* / Q000494:pharmacology
chemical_list: D000470:Alkaloids; D000975:Antioxidants; D007004:Hypoglycemic Agents; D010936:Plant Extracts; D006861:Hydrogen Peroxide
references: Free Radic Biol Med. 1993 Mar;14(3):303-11
references: 8458588
references: Asian Pac J Trop Med. 2011 Jun;4(6):475-7
references: 21771702
references: J Ethnopharmacol. 1999 Nov 30;67(3):367-72
references: 10617074
references: J Neurosci. 2003 Aug 13;23(19):7337-42
references: 12917367
references: Diabetes. 1995 Mar;44(3):306-13
references: 7533732
references: BMC Complement Altern Med. 2013 Jul 10;13:166
references: 23837445
references: Food Chem. 2008 Jun 1;108(3):1002-7
references: 26065764
references: Anal Bioanal Chem. 2011 May;400(5):1451-8
references: 21512895
references: Toxicol Lett. 2007 Sep 10;173(2):80-7
references: 17689207
references: J Agric Food Chem. 2001 Nov;49(11):5165-70
references: 11714298
references: BMC Complement Altern Med. 2003 Sep 2;3:4
references: 12950994
references: Mol Cell Endocrinol. 2004 Dec 30;228(1-2):121-8
references: 15541576
references: J Agric Food Chem. 2008 Nov 12;56(21):9967-74
references: 18850714
references: Asian Pac J Trop Biomed. 2012 May;2(5):411-20
references: 23569941
references: Mol Cell Endocrinol. 2012 Nov 25;364(1-2):1-27
references: 22885162
references: J Immunol Methods. 1983 Dec 16;65(1-2):55-63
references: 6606682
references: J Ethnopharmacol. 2004 May;92(1):1-21
references: 15099842
references: Food Chem Toxicol. 2011 Aug;49(8):1806-13
references: 21570444
references: J Ethnopharmacol. 2012 Oct 31;144(1):22-32
references: 22954496
references: J Ethnopharmacol. 2001 Aug;76(3):269-77
references: 11448549
references: Med J Malaysia. 2010 Sep;65(3):180-6
references: 21939164
references: J Nat Prod. 1983 Jul-Aug;46(4):517-27
references: 6631435
references: Pak J Biol Sci. 2013 Oct 15;16(20):1212-5
references: 24506026
references: J Biol Chem. 2004 Jun 4;279(23):24226-35
references: 15024017
references: J Am Chem Soc. 1973 Jul 25;95(15):4990-5
references: 4741281