title: Photo-activatable ternary complex based on a multifunctional shielding material for targeted shRNA delivery in cancer treatment.
abstract: A photo-activatable ternary complex (PTC) consisting of multifunctional shielding material (MSM) with photosensitizer (PS)-conjugated chondroitin sulfate and polyethyleneimine based binary complexes containing epidermal growth factor receptor (EGFR)-shRNA delivery for CD44 targeted cancer therapy has been developed. The PTC has a negative surface charge of -37.9 mV, and a size of approximately 90 nm, and the ternary complexes were found to be stable against plasma proteins. The stable nanostructure of PTC especially could enable CD44-receptor mediated tumor-targeted delivery and PS-mediated endosomal disruption for efficient gene silencing, and an enhanced rate of cancer cell death was achieved both in vitro and in vivo. This suggests that PTC could represent a promising strategy for the delivery of other therapeutic genes for cancer therapy.
authors: Department of Biotechnology, The Catholic University of Korea, Bucheon-si, Gyeonggi-do 420-743, Republic of Korea.
authors: Sin-Jung
authors: SJ
authors: Park
authors: Wooram
authors: W
authors: Park
authors: Kun
authors: K
authors: Na
mesh_terms: D000818:Animals; D016923:Cell Death; D002470:Cell Survival / Q000187:drug effects; D055598:Chemical Phenomena; D004705:Endocytosis / Q000187:drug effects; D066246:ErbB Receptors / Q000235:genetics / Q000378:metabolism; D020868:Gene Silencing; D018014:Gene Transfer Techniques; D015316:Genetic Therapy / Q000379:methods*; D045325:HCT116 Cells; D006801:Humans; D006820:Hyaluronic Acid / Q000494:pharmacology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009369:Neoplasms / Q000628:therapy*; D011094:Polyethyleneimine / Q000737:chemistry / Q000494:pharmacology; D034741:RNA, Small Interfering / Q000235:genetics* / Q000378:metabolism; D014162:Transfection
chemical_list: D034741:RNA, Small Interfering; D011094:Polyethyleneimine; D006820:Hyaluronic Acid; D066246:ErbB Receptors
keywords: Cancer therapy; EGFR-shRNA; Gene delivery; Photosensitizer; Ternary complex