title: Rapid and sensitive phenotypic marker detection on breast cancer cells using surface-enhanced Raman scattering (SERS) imaging.
abstract: We report a surface-enhanced Raman scattering (SERS)-based cellular imaging technique to detect and quantify breast cancer phenotypic markers expressed on cell surfaces. This technique involves the synthesis of SERS nano tags consisting of silica-encapsulated hollow gold nanospheres (SEHGNs) conjugated with specific antibodies. Hollow gold nanospheres (HGNs) enhance SERS signal intensity of individual particles by localizing surface electromagnetic fields through pinholes in the hollow particle structures. This capacity to enhance imaging at the level of single molecules permits the use of HGNs to detect specific biological markers expressed in living cancer cells. In addition, silica encapsulation greatly enhances the stability of nanoparticles. Here we applied a SERS-based imaging technique using SEHGNs in the multiplex imaging of three breast cancer cell phenotypes. Expression of epidermal growth factor (EGF), ErbB2, and insulin-like growth factor-1 (IGF-1) receptors were assessed in the MDA-MB-468, KPL4 and SK-BR-3 human breast cancer cell lines. SERS imaging technology described here can be used to test the phenotype of a cancer cell and quantify proteins expressed on the cell surface simultaneously. Based on results, this technique may enable an earlier diagnosis of breast cancer than is currently possible and offer guidance in treatment.
authors: Department of Bionano Engineering, Hanyang University, Ansan 426-791, South Korea.
authors: Sangyeop
authors: S
authors: Lee
authors: Hyangah
authors: H
authors: Chon
authors: Jiyoung
authors: J
authors: Lee
authors: Juhui
authors: J
authors: Ko
authors: Bong Hyun
authors: BH
authors: Chung
authors: Dong Woo
authors: DW
authors: Lim
authors: Jaebum
authors: J
authors: Choo
mesh_terms: D055910:Antibodies, Immobilized; D014408:Biomarkers, Tumor / Q000032:analysis; D015374:Biosensing Techniques / Q000191:economics / Q000379:methods; D001940:Breast / Q000473:pathology*; D001943:Breast Neoplasms / Q000175:diagnosis* / Q000473:pathology; D045744:Cell Line, Tumor; D004815:Epidermal Growth Factor / Q000032:analysis*; D005260:Female; D006046:Gold / Q000737:chemistry; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000032:analysis*; D054118:Nanospheres / Q000737:chemistry; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000032:analysis*; D012822:Silicon Dioxide / Q000737:chemistry; D013059:Spectrum Analysis, Raman / Q000379:methods*; D013499:Surface Properties
chemical_list: D055910:Antibodies, Immobilized; D014408:Biomarkers, Tumor; D004815:Epidermal Growth Factor; D006046:Gold; D007334:Insulin-Like Growth Factor I; D018719:Erb-b2 Receptor Tyrosine Kinases; D012822:Silicon Dioxide; C508053:ERBB2 protein, human
keywords: Breast cancer; Cell phenotype; Multiplex detection; SERS nano tag; Surface-enhanced Raman scattering