title: Fluoride releasing and enamel demineralization around orthodontic brackets by fluoride-releasing composite containing nanoparticles.
abstract: BACKGROUND
  Fluoride-containing materials have been suggested to control enamel demineralization around orthodontic brackets during the treatment with fixed appliances. The improvement of their properties has been made through innovations, such as the application of nanotechnology by incorporation of nanofillers.
  
  
  OBJECTIVE
  This in vitro study evaluated the capacity of fluoride releasing and enamel demineralization inhibition of fluoride-releasing nanofilled cement around orthodontic brackets using an artificial caries biofilm model.
  
  
  METHODS
  Forty bovine enamel discs were selected by evaluating surface microhardness and randomized into four groups (n = 10): non-fluoride-releasing microfilled composite, fluoride-releasing microfilled composite, resin-modified glass ionomer cement (RMGI), and fluoride-releasing nanofilled composite (FN). After brackets bonding in each disc, the specimens were subjected to a cariogenic challenge through a Streptococcus mutans biofilm model. After the experimental period, the biofilm formed around the brackets was collected for fluoride analysis and the mineral loss around the brackets was determined by integrated demineralization via cross-sectional microhardness measurement at 20 and 70 μm from the bracket margin. Additionally, samples of each group were subjected to energy-dispersive X-ray spectroscopy (EDX) analysis examined under a scanning electron microscopy (SEM). ANOVA followed by Tukey test were applied for fluoride concentration and mineral loss data, respectively.
  
  
  RESULTS
  At both distances, only RMGI statistically differed from the other groups presenting the lowest demineralization, although there was a trend to a lower demineralization of enamel around brackets in FN group. Similar condition was found to fluoride concentration and EDX/SEM analysis.
  
  
  CONCLUSIONS
  Under the cariogenic exposure condition of this study, the fluoride-releasing nanofilled material had similar performance to fluoride-releasing microfilled materials.
  
  
  CONCLUSIONS
  The presence of nanofillers in the fluoride-releasing materials studied did not promote further benefits against caries lesion development around brackets and presented inferior demineralization inhibition than the resin-modified glass ionomer material.
authors: Post-graduate Program in Dentistry, Faculty of Pharmacy, Dentistry and Nursing, Federal University of Ceará, 944 Cap Francisco Pedro Street-Rodolfo Teófilo, Fortaleza, Ceará, 60430-170, Brazil.
authors: Mary A S
authors: MAS
authors: Melo
authors: Post-graduate Program in Dentistry, Faculty of Pharmacy, Dentistry and Nursing, Federal University of Ceará, 944 Cap Francisco Pedro Street-Rodolfo Teófilo, Fortaleza, Ceará, 60430-170, Brazil.
authors: Weslanny A
authors: WA
authors: Morais
authors: School of Dentistry, University of Fortaleza, Washington Soares Avenue,1321, Fortaleza, Ceará, 60811-905, Brazil.
authors: Vanara F
authors: VF
authors: Passos
authors: Post-graduate Program in Dentistry, Faculty of Pharmacy, Dentistry and Nursing, Federal University of Ceará, 944 Cap Francisco Pedro Street-Rodolfo Teófilo, Fortaleza, Ceará, 60430-170, Brazil.
authors: Juliana P M
authors: JPM
authors: Lima
authors: Post-graduate Program in Dentistry, Faculty of Pharmacy, Dentistry and Nursing, Federal University of Ceará, 944 Cap Francisco Pedro Street-Rodolfo Teófilo, Fortaleza, Ceará, 60430-170, Brazil. lidianykarla@yahoo.com.
authors: Lidiany K A
authors: LKA
authors: Rodrigues
mesh_terms: D005459:Fluorides / Q000493:pharmacokinetics*; D008855:Microscopy, Electron, Scanning; D053758:Nanoparticles; D016910:Orthodontic Brackets; D013052:Spectrometry, X-Ray Emission; D017001:Tooth Demineralization
chemical_list: D005459:Fluorides
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