title: Antimicrobial effect and membrane-active mechanism of tea polyphenols against Serratia marcescens.
abstract: In this study, we investigated the antimicrobial effect of tea polyphenols (TP) against Serratia marcescens and examined the related mechanism. Morphology changes of S. marcescens were first observed by transmission electron microscopy after treatment with TP, which indicated that the primary inhibition action of TP was to damage the bacterial cell membranes. The permeability of the outer and inner membrane of S. marcescens dramatically increased after TP treatment, which caused severe disruption of cell membrane, followed by the release of small cellular molecules. Furthermore, a proteomics approach based on two-dimensional gel electrophoresis and MALDI-TOF/TOF MS analysis was used to study the difference of membrane protein expression in the control and TP treatment S. marcescens. The results showed that the expression of some metabolism enzymes and chaperones in TP-treated S. marcescens significantly increased compared to the untreated group, which might result in the metabolic disorder of this bacteria. Taken together, our results first demonstrated that TP had a significant growth inhibition effect on S. marcescens through cell membrane damage.
authors: College of Chemistry, Chemical Engineering and Food Safety, Bohai University, 19 KeJi Road, Jinzhou, 121013, Liaoning, People's Republic of China.
authors: Shumin
authors: S
authors: Yi
authors: Wei
authors: W
authors: Wang
authors: Fengling
authors: F
authors: Bai
authors: Junli
authors: J
authors: Zhu
authors: Jianrong
authors: J
authors: Li
authors: Xuepeng
authors: X
authors: Li
authors: Yongxia
authors: Y
authors: Xu
authors: Tong
authors: T
authors: Sun
authors: Yutang
authors: Y
authors: He
mesh_terms: D000900:Anti-Bacterial Agents / Q000302:isolation & purification / Q000494:pharmacology*; D002462:Cell Membrane / Q000187:drug effects* / Q000502:physiology / Q000648:ultrastructure; D002463:Cell Membrane Permeability / Q000187:drug effects; D015180:Electrophoresis, Gel, Two-Dimensional; D008660:Metabolism / Q000187:drug effects; D046529:Microscopy, Electron, Transmission; D059808:Polyphenols / Q000302:isolation & purification / Q000494:pharmacology*; D020543:Proteome / Q000032:analysis; D012706:Serratia marcescens / Q000187:drug effects* / Q000648:ultrastructure; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013662:Tea / Q000737:chemistry*
chemical_list: D000900:Anti-Bacterial Agents; D059808:Polyphenols; D020543:Proteome; D013662:Tea
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