title: Electrogenerated chemiluminescence detector based on Ru(bpy)3(2+) immobilized in cation exchange resin for high-performance liquid chromatography: An approach to stable detection.
abstract: In this work, an electrogenerated chemiluminescence (ECL) detector with improved stability was developed for high-performance liquid chromatography (HPLC) detection of hydrochlorothiazide (HCTZ). The detector was prepared by packing cation exchanged resin particles in a glass tube, followed by inserting Pt wires (working electrode) in this tube and sealing. The leakage of Ru(bpy)3(2+) from the resin was compensated by adding a small amount of Ru(bpy)3(2+) in the mobile phase. Factors affected the performance of the proposed ECL detector were investigated. Under the optimal conditions, the ECL intensity has a linear relationship with the concentration of HCTZ in the range of 5.0 × 10(-8) g mL(-1)-2.5 × 10(-5) g mL(-1) and the detection limit was 2.0 × 10(-8) g mL(-1) (S/N=3). Application of the detector to the analysis of HCTZ in human serum proved feasible.
authors: Mineral Resources Chemistry Key Laboratory of Sichuan Higher Education Institutions, College of Material and Chemistry & Chemical Engineering, Chengdu University of Technology, Chengdu, Sichuan 610059, People's Republic of China. Electronic address: syh19750205@126.com.
authors: Yonghua
authors: Y
authors: Sun
authors: Zhujun
authors: Z
authors: Zhang
authors: Xinfeng
authors: X
authors: Zhang
mesh_terms: D015082:2,2'-Dipyridyl / Q000031:analogs & derivatives* / Q000737:chemistry; D002411:Cation Exchange Resins / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation*; D056831:Coordination Complexes; D004232:Diuretics / Q000097:blood*; D004867:Equipment Design; D006801:Humans; D006852:Hydrochlorothiazide / Q000097:blood*; D057230:Limit of Detection; D049408:Luminescent Agents / Q000737:chemistry*; D008163:Luminescent Measurements / Q000295:instrumentation; D017975:Ruthenium Compounds
chemical_list: D015082:2,2'-Dipyridyl; D002411:Cation Exchange Resins; D056831:Coordination Complexes; D004232:Diuretics; D006852:Hydrochlorothiazide; D049408:Luminescent Agents; C547232:tris(2,2-bipyridine)-ruthenium(II); D017975:Ruthenium Compounds
keywords: Cation exchange resin; Electrogenerated chemiluminescence detector; High performance liquid chromatography; Hydrochlorothiazide; Ru