title: The contribution of common rpsL mutations in Escherichia coli to sensitivity to ribosome targeting antibiotics.
abstract: Point mutations in the rpsL gene encoding ribosomal protein S12 can generate resistance to streptomycin, resulting in rapid emergence of resistance to this antibiotic during treatment. In this work, we demonstrate that while spontaneous rpsL mutants in Escherichia coli are resistant to streptomycin, they are more sensitive to the ribosome-targeting antibiotics chloramphenicol, tetracycline and erythromycin. Moreover, combinations of these antibiotics, even in low concentrations were enough to achieve complete growth inhibition of both wild type and rpsL mutant strains. Thus, combining ribosome-targeting drugs can be used as a new treatment strategy that may be effective against streptomycin-resistant ribosome mutants.
authors: Department of Molecular Microbiology and Biotechnology, George S. Wise Faculty of Life Sciences, Tel Aviv University, Ramat Aviv 69978, Israel.
authors: Gidi
authors: G
authors: Pelchovich
authors: Rachel
authors: R
authors: Schreiber
authors: Alina
authors: A
authors: Zhuravlev
authors: Uri
authors: U
authors: Gophna
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002701:Chloramphenicol / Q000494:pharmacology; D024881:Drug Resistance, Bacterial; D004917:Erythromycin / Q000494:pharmacology; D004926:Escherichia coli / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development; D029968:Escherichia coli Proteins / Q000235:genetics*; D008826:Microbial Sensitivity Tests; D017354:Point Mutation; D000091243:Ribosomal Protein S9; D012269:Ribosomal Proteins / Q000235:genetics*; D012270:Ribosomes / Q000187:drug effects* / Q000235:genetics; D013307:Streptomycin / Q000494:pharmacology*; D013752:Tetracycline / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D029968:Escherichia coli Proteins; D000091243:Ribosomal Protein S9; D012269:Ribosomal Proteins; C000717190:RpsI protein, E coli; C506766:RpsL protein, E coli; D004917:Erythromycin; D002701:Chloramphenicol; D013752:Tetracycline; D013307:Streptomycin
keywords: Antibiotic resistance; Chloramphenicol; Erythromycin; Gentamicin; Ribosomal protein S12; Tetracycline; rpsL