title: Developmental changes in the in vitro activated regenerative activity of primitive mammary epithelial cells.
abstract: Many normal adult tissues contain rare stem cells with extensive self-maintaining regenerative potential. During development, the stem cells of the hematopoietic and neural systems undergo intrinsically specified changes in their self-renewal potential. In the mouse, mammary stem cells with transplantable regenerative activity are first detectable a few days before birth. They share some phenotypic properties with their adult counterparts but are enriched in a subpopulation that displays a distinct gene expression profile. Here we show that fetal mammary epithelial cells have a greater direct and inducible growth potential than their adult counterparts. The latter feature is revealed in a novel culture system that enables large numbers of in vitro clonogenic progenitors as well as mammary stem cells with serially transplantable activity to be produced within 7 days from single fetal or adult input cells. We further show that these responses are highly dependent on novel factors produced by fibroblasts. These findings provide new avenues for elucidating mechanisms that regulate normal mammary epithelial stem cell properties at the single-cell level, how these change during development, and how their perturbation may contribute to transformation.
authors: Terry Fox Laboratory, British Columbia Cancer Agency, Vancouver, British Columbia, Canada.
authors: Maisam
authors: M
authors: Makarem
authors: Nagarajan
authors: N
authors: Kannan
authors: Long V
authors: LV
authors: Nguyen
authors: David J H F
authors: DJ
authors: Knapp
authors: Sneha
authors: S
authors: Balani
authors: Michael D
authors: MD
authors: Prater
authors: John
authors: J
authors: Stingl
authors: Afshin
authors: A
authors: Raouf
authors: Oksana
authors: O
authors: Nemirovsky
authors: Peter
authors: P
authors: Eirew
authors: Connie J
authors: CJ
authors: Eaves
mesh_terms: D016475:3T3 Cells; D000818:Animals; D004847:Epithelial Cells / Q000166:cytology* / Q000502:physiology; D005260:Female; D007150:Immunohistochemistry; D008321:Mammary Glands, Animal / Q000166:cytology* / Q000502:physiology; D051379:Mice
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