title: Evaluation of inhibitory effect of phlorotannins from Ecklonia cava on triglyceride accumulation in adipocyte.
abstract: In the present study, a methanolic extract of Ecklonia cava and its solvent-partitioned fractions were evaluated for their antiadipogenic effect in 3T3-L1 adipocytes. One of them, the n-BuOH fraction, effectively reduced lipid accumulation and glucose consumption. In addition, the presence of the n-BuOH fraction in adipocytes suppressed the regulations of adipogenic transcription factors, PPARγ and SREBP1c, and adipogenic specific genes, FABP4, FABP1, FAS, LPL, HSL, and ACS1. Further purification of n-BuOH fractions led to the isolation of six phlorotannins (1-6). The six phlorotannins effectively suppressed triglyceride accumulation. Comparative analysis showed that lipid accumulation in adipocytes was dramatically attenuated in the presence of eckstolonol (4).
authors: Division of Marine Environment and Bioscience, College of Ocean Science and Technology, Korea Maritime University , Busan 606-791, Republic of Korea.
authors: Haejin
authors: H
authors: Kim
authors: Chang-Suk
authors: CS
authors: Kong
authors: Jung Im
authors: JI
authors: Lee
authors: Hojun
authors: H
authors: Kim
authors: Seungoh
authors: S
authors: Baek
authors: Youngwan
authors: Y
authors: Seo
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000187:drug effects* / Q000378:metabolism*; D050156:Adipogenesis / Q000235:genetics; D000818:Animals; D004146:Dioxanes / Q000494:pharmacology; D015870:Gene Expression / Q000187:drug effects; D005947:Glucose / Q000378:metabolism; D006576:Heterocyclic Compounds, 4 or More Rings / Q000494:pharmacology; D051379:Mice; D000459:Phaeophyceae / Q000737:chemistry*; D013634:Tannins / Q000494:pharmacology*; D014280:Triglycerides / Q000378:metabolism*
chemical_list: D004146:Dioxanes; D006576:Heterocyclic Compounds, 4 or More Rings; D013634:Tannins; D014280:Triglycerides; C507565:eckstolonol; D005947:Glucose