title: An innovative strategy for direct electrochemical detection of microRNA biomarkers.
abstract: We report an electrochemical method for direct, reagentless, and label-free detection of microRNA, based on a conjugated copolymer, poly(5-hydroxy-1,4-naphthoquinone-co-5-hydroxy-2-carboxyethyl-1,4-naphthoquinone), acting as hybridization transducer. Hybridization between the oligonucleotide capture probe and a microRNA target of 22 base pairs generates an increase in the redox current ("signal-on"), which is evidenced by square wave voltammetry. Selectivity is good, with little hybridization for non-complementary targets, and the limit of detection reaches 650 fM. It is also evidenced that this sensitivity benefits from the high affinity of DNA for RNA.
authors: Univ. Paris Diderot, Sorbonne Paris Cité, ITODYS, UMR 7086 CNRS, 15 rue J-A de Baïf, 75205, Paris Cedex 13, France.
authors: H V
authors: HV
authors: Tran
authors: B
authors: B
authors: Piro
authors: S
authors: S
authors: Reisberg
authors: G
authors: G
authors: Anquetin
authors: H T
authors: HT
authors: Duc
authors: M C
authors: MC
authors: Pham
mesh_terms: D015415:Biomarkers / Q000737:chemistry; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D004563:Electrochemistry; D035683:MicroRNAs / Q000737:chemistry* / Q000235:genetics; D009693:Nucleic Acid Hybridization
chemical_list: D015415:Biomarkers; D035683:MicroRNAs