title: High molecular weight forms of mammalian respiratory chain complex II.
abstract: Mitochondrial respiratory chain is organised into supramolecular structures that can be preserved in mild detergent solubilisates and resolved by native electrophoretic systems. Supercomplexes of respiratory complexes I, III and IV as well as multimeric forms of ATP synthase are well established. However, the involvement of complex II, linking respiratory chain with tricarboxylic acid cycle, in mitochondrial supercomplexes is questionable. Here we show that digitonin-solubilised complex II quantitatively forms high molecular weight structures (CIIhmw) that can be resolved by clear native electrophoresis. CIIhmw structures are enzymatically active and differ in electrophoretic mobility between tissues (500 - over 1000 kDa) and cultured cells (400-670 kDa). While their formation is unaffected by isolated defects in other respiratory chain complexes, they are destabilised in mtDNA-depleted, rho0 cells. Molecular interactions responsible for the assembly of CIIhmw are rather weak with the complexes being more stable in tissues than in cultured cells. While electrophoretic studies and immunoprecipitation experiments of CIIhmw do not indicate specific interactions with the respiratory chain complexes I, III or IV or enzymes of the tricarboxylic acid cycle, they point out to a specific interaction between CII and ATP synthase.
authors: Department of Bioenergetics, Institute of Physiology Academy of Sciences of the Czech Republic, Prague, Czech Republic.
authors: Nikola
authors: N
authors: Kovářová
authors: Tomáš
authors: T
authors: Mráček
authors: Hana
authors: H
authors: Nůsková
authors: Eliška
authors: E
authors: Holzerová
authors: Marek
authors: M
authors: Vrbacký
authors: Petr
authors: P
authors: Pecina
authors: Kateřina
authors: K
authors: Hejzlarová
authors: Katarína
authors: K
authors: Kľučková
authors: Jakub
authors: J
authors: Rohlena
authors: Jiri
authors: J
authors: Neuzil
authors: Josef
authors: J
authors: Houštěk
mesh_terms: D000818:Animals; D002460:Cell Line; D004579:Electron Transport; D045222:Electron Transport Chain Complex Proteins / Q000737:chemistry / Q000378:metabolism; D042963:Electron Transport Complex II / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D053858:Metabolic Networks and Pathways; D008928:Mitochondria / Q000235:genetics / Q000378:metabolism; D008970:Molecular Weight; D009928:Organ Specificity; D010085:Oxidative Phosphorylation; D011485:Protein Binding
chemical_list: D045222:Electron Transport Chain Complex Proteins; D042963:Electron Transport Complex II
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