title: Serum proteins in chronic hepatitis B patients treated with peginterferon alfa-2b.
abstract: OBJECTIVE
  To study the differential protein profile in serum of hepatitis B patients.
  
  
  METHODS
  Serum samples were obtained from patients with chronic hepatitis B who were receiving peginterferon alfa-2b. The serum samples were subjected to albumin depletion and analyzed by two-dimensional gel electrophoresis (2-DE). Differentially expressed protein spots were identified by electrospray ionization-quadrupole time-of-flight mass spectrometry. Alpha-2-HS-glycoprotein, complement component C3c and CD5 antigen were further analyzed by an enzyme-linked immunosorbent assay and immunonephelometry.
  
  
  RESULTS
  Nineteen patients with HBeAg-positive chronic hepatitis B (CHB) were studied. These patients were followed for at least 1 year after treatment and were classified according to their treatment response: responders (n = 9) and non-responders (n = 10). 2-DE and MS/MS analysis were performed to compare the serum proteins before initiating peginterferon alfa-2b. From the quantitative analysis of the 2-D gel, 7 proteins were detected between the two groups at different levels before treatment. Among these potential candidates, serum levels of alpha-2-HS-glycoprotein, complement component C3c and CD5 antigen-like precursor were further analyzed. In the validation phase, 23 subjects, 9 sustained responders and 14 non-responders, were recruited. Interestingly, the levels of alpha-2-HS-glycoprotein and complement component C3c were elevated in the serum of the non-responders compared to the responders.
  
  
  CONCLUSIONS
  Serum alpha-2-HS-glycoprotein and complement component C3c may be potential serum biomarkers in predicting the treatment response of peginterferon alfa-2b in patients with CHB prior to treatment.
authors: Medical Microbiology, Interdisciplinary Program, Graduate School, Chulalongkorn University, Bangkok 10330, Thailand.
authors: Sunida
authors: S
authors: Kuakarn
authors: Poorichaya
authors: P
authors: SomParn
authors: Pisit
authors: P
authors: Tangkijvanich
authors: Varocha
authors: V
authors: Mahachai
authors: Visith
authors: V
authors: Thongboonkerd
authors: Nattiya
authors: N
authors: Hirankarn
mesh_terms: D000328:Adult; D000998:Antiviral Agents / Q000627:therapeutic use*; D015415:Biomarkers / Q000097:blood; D001798:Blood Proteins / Q000032:analysis*; D018956:CD5 Antigens / Q000097:blood; D015932:Complement C3c / Q000032:analysis; D015180:Electrophoresis, Gel, Two-Dimensional; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006514:Hepatitis B Surface Antigens / Q000097:blood; D019694:Hepatitis B, Chronic / Q000097:blood / Q000175:diagnosis / Q000188:drug therapy*; D006801:Humans; D000077190:Interferon alpha-2; D016898:Interferon-alpha / Q000627:therapeutic use*; D008297:Male; D011092:Polyethylene Glycols / Q000627:therapeutic use*; D040901:Proteomics / Q000379:methods; D011994:Recombinant Proteins / Q000627:therapeutic use; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry; D016896:Treatment Outcome; D055815:Young Adult; D060749:alpha-2-HS-Glycoprotein / Q000032:analysis
chemical_list: C556354:AHSG protein, human; D000998:Antiviral Agents; D015415:Biomarkers; D001798:Blood Proteins; D018956:CD5 Antigens; D006514:Hepatitis B Surface Antigens; D000077190:Interferon alpha-2; D016898:Interferon-alpha; D011994:Recombinant Proteins; D060749:alpha-2-HS-Glycoprotein; D011092:Polyethylene Glycols; D015932:Complement C3c; C417083:peginterferon alfa-2b
keywords: Alpha-2-HS-glycoprotein; Chronic hepatitis B; Peginterferon alfa-2b; Proteomics; Serum
references: Recent Pat Antiinfect Drug Discov. 2006 Jan;1(1):85-94
references: 18221137
references: J Am Soc Nephrol. 2010 Mar;21(3):406-12
references: 20150538
references: Anal Biochem. 1976 May 7;72:248-54
references: 942051
references: Proteomics. 2005 Aug;5(13):3292-303
references: 16052620
references: Gastroenterology. 2010 Jan;138(1):165-72.e1-3
references: 19782084
references: Nat Rev Gastroenterol Hepatol. 2009 Oct;6(10):565-7
references: 19789570
references: World J Gastroenterol. 2007 May 14;13(18):2554-67
references: 17552002
references: Proteomics. 2003 May;3(5):666-74
references: 12748946
references: BMC Gastroenterol. 2007 Mar 29;7:15
references: 17394649
references: Biotechniques. 1996 Jan;20(1):30-2
references: 8770400
references: Proteomics. 2003 May;3(5):601-9
references: 12748940
references: Gastroenterology. 2006 Jun;130(7):2189-97
references: 16762639
references: Hepatol Int. 2007 Dec;1(4):415-30
references: 19669337
references: Hepatology. 2011 Jun;53(6):1809-18
references: 21381069
references: Drugs. 2009 Nov 12;69(16):2167-77
references: 19852523
references: Gut. 2007 May;56(5):699-705
references: 17127704
references: Sci China Life Sci. 2011 Jan;54(1):39-47
references: 21253869
references: Cell Tissue Res. 1987 Apr;248(1):33-41
references: 3552239
references: Rapid Commun Mass Spectrom. 2001;15(16):1416-21
references: 11507753
references: BMC Gastroenterol. 2010 Oct 13;10:115
references: 20939925
references: Int J Rheum Dis. 2010 Feb 1;13(1):75-81
references: 20374388
references: Int J Mol Sci. 2011;12(6):3846-56
references: 21747711
references: Zhonghua Gan Zang Bing Za Zhi. 2007 Mar;15(3):187-91
references: 17407708
references: J Clin Virol. 2009 Oct;46(2):117-23
references: 19651540
references: Eur J Gastroenterol Hepatol. 2002 Apr;14(4):389-94
references: 11943951
references: Ann Clin Biochem. 2010 Nov;47(Pt 6):549-53
references: 20926473
references: Electrophoresis. 2004 May;25(9):1327-33
references: 15174055
references: Immunity. 2003 Feb;18(2):217-27
references: 12594949
references: Gastroenterology. 2009 Dec;137(6):2002-9
references: 19737568
references: Blood Transfus. 2010 Jun;8 Suppl 3:s105-12
references: 20606740
references: Cell Mol Immunol. 2006 Oct;3(5):333-40
references: 17092430
references: Proteomics. 2005 Nov;5(17):4581-8
references: 16240287
references: Anal Bioanal Chem. 2007 Jun;388(3):637-43
references: 17447055
references: World J Gastroenterol. 2005 Sep 28;11(36):5721-4
references: 16237774