title: Cross-reactivity of human monoclonal antibodies generated with peripheral blood lymphocytes from dengue patients with Japanese encephalitis virus.
abstract: BACKGROUND
  Hybridomas that produce human monoclonal antibodies (HuMAbs) against Dengue virus (DV) had been prepared previously using peripheral blood lymphocytes from patients with DV during the acute and convalescent phases of a secondary infection. Anti-DV envelope glycoprotein (E) 99 clones, anti-DV premembrane protein (prM) 8 clones, and anti-DV nonstructural protein 1 (NS1) 4 clones were derived from four acute-phase patients, and anti-DV E 2 clones, anti-DV prM 2 clones, and anti-DV NS1 8 clones were derived from five convalescent-phase patients.
  
  
  RESULTS
  In the present study, we examined whether these clones cross-reacted with Japanese encephalitis virus (JEV), which belongs to the same virus family. Forty-six of the above-described 99 (46/99) anti-E, 0/8 anti-prM, and 2/4 anti-NS1 HuMAbs from acute-phase, and 0/2 anti-E, 0/2 anti-prM, and 5/8 anti-NS1 HuMAbs from convalescent-phase showed neutralizing activity against JEV. Thus, most of the anti-E and anti-NS1 (but not the anti-prM) antibodies cross-reacted with JEV and neutralized this virus. Interestingly, 3/46 anti-E HuMAbs derived from acute-phase patients and 3/5 anti-NS1 HuMAbs from convalescent-phase patients showed particularly high neutralizing activity against JEV. Consequently, the HuMAbs showing neutralization against JEV mostly consisted of two populations: one was HuMAbs recognizing DV E and showing neutralization activity against all four DV serotypes (complex-type) and the other was HuMAbs recognizing DV NS1 and showing subcomplex-type cross-reaction with DV.
  
  
  CONCLUSIONS
  Anti-DV E from acute phase (46/99) and anti-DV NS1 (7/12) indicate neutralizing activity against JEV. In particular, three of 46 anti-DV E clones from acute phase and three of five anti-NS1 clones from convalescent phase showed strong neutralizing activity against JEV.
authors: Center of Excellence for Antibody Research, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand ; Department of Microbiology and Immunology, Bangkok, Thailand ; JST/JICA, Science and Technology Research Partnership for Sustainable Development, Tokyo, Japan.
authors: Chonlatip
authors: C
authors: Pipattanaboon
authors: Tadahiro
authors: T
authors: Sasaki
authors: Mitsuhiro
authors: M
authors: Nishimura
authors: Chayanee
authors: C
authors: Setthapramote
authors: Pannamthip
authors: P
authors: Pitaksajjakul
authors: Pornsawan
authors: P
authors: Leaungwutiwong
authors: Kriengsak
authors: K
authors: Limkittikul
authors: Orapim
authors: O
authors: Puiprom
authors: Mikiko
authors: M
authors: Sasayama
authors: Panjaporn
authors: P
authors: Chaichana
authors: Tamaki
authors: T
authors: Okabayashi
authors: Takeshi
authors: T
authors: Kurosu
authors: Ken-Ichiro
authors: K
authors: Ono
authors: Pongrama
authors: P
authors: Ramasoota
authors: Kazuyoshi
authors: K
authors: Ikuta
keywords: Dengue virus; Japanese encephalitis virus; envelope; human monoclonal antibody; nonstructural protein 1; viral neutralization
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