title: Isocyanate-mediated covalent immobilization of Mucor miehei lipase onto SBA-15 for transesterification reaction.
abstract: Mucor miehei lipase (Mm-L) covalently bind on a hexagonally ordered silica SBA-15 (Santa Barbara Amorphous), previously functionalized with isocyanate moieties, was examined as biocatalyst for transesterification of colza oil with methanol. The isocyanate-mesoporous silica (NCO-SBA-15) was obtained by condensation of silanol with triethoxysilane propyl isocyanate (TPI). The efficiency of the functionalization has been evidenced by infrared, (29)Si and (13)C NMR spectroscopies. The substrate provided a moderate hydrophobic microenvironment together with reactive sites for chemical immobilization of the enzyme. The biocatalyst containing 0.28 g of Mm-L per gram of support afforded a high level of transesterification activity (yield up to 80%) while using 1:1 molar ratio of methanol/colza oil and small amount of water. The biocatalyst showed higher operational stability than the corresponding physisorbed enzyme since it can be reused 6 times against 2 consecutive runs for the physisorbed enzyme.
authors: Université de Lorraine, SRSMC, UMR 7565, F-54506 Vandoeuvre-lès-Nancy cedex, France; CNRS, SRSMC, UMR 7565, F-54506 Vandoeuvre-lès-Nancy cedex, France.
authors: N
authors: N
authors: Canilho
authors: J
authors: J
authors: Jacoby
authors: A
authors: A
authors: Pasc
authors: C
authors: C
authors: Carteret
authors: F
authors: F
authors: Dupire
authors: M J
authors: MJ
authors: Stébé
authors: J L
authors: JL
authors: Blin
mesh_terms: D000327:Adsorption; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism*; D004951:Esterification; D017953:Isocyanates; D008049:Lipase / Q000737:chemistry / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D009089:Mucor / Q000201:enzymology; D053838:Scattering, Small Angle; D012822:Silicon Dioxide; D017550:Spectroscopy, Fourier Transform Infrared; D013499:Surface Properties; D014961:X-Ray Diffraction
chemical_list: D004800:Enzymes, Immobilized; D017953:Isocyanates; C509969:SBA-15; D012822:Silicon Dioxide; D008049:Lipase
keywords: Chemical immobilization; Functionalization; Lipase; Mesoporous silica; Reusability; Transesterification