title: Biochemical modulation of growth, lipid quality and productivity in mixotrophic cultures of Chlorella sorokiniana.
abstract: The genus Chlorella is a widely employed microalga for biodiesel, as it can be grown using photo/mixo/heterotrophic mode of cultivation. The present investigation was undertaken with the hypothesis that addition of different substrates (amino acids, carbon sources, vitamins) along with reducing agents may aid in diverting Acetyl CoA to malonyl CoA or fatty acid biosynthesis, under mixotrophic conditions in Chlorella sorokiniana. Preliminary investigations undertaken with two reducing agents individually (sodium thiosulphate and methyl viologen) along with selected substrates revealed the promise of sodium thiosulphate (1%) in enhancing lipid accumulation significantly. Further, the role of inclusion of twelve substrates and sodium thiosulphate revealed that supplementation with tryptophan (0.1%) recorded 57.28% enhancement in lipid productivity on 4(th) day. Highest values of lipid productivity of 33% were recorded on 8(th) day in 0.1% glucose supplemented medium containing sodium thiosulphate. Fatty Acid Methyl Ester (FAME) profiles generated revealed significant reduction in the content of Poly unsaturated fatty acids (PUFA) and enhanced Mono unsaturated fatty acids (MUFA) (especially oleic acid) in the treatments involving tryptophan, Vitamin B12, sodium pyruvate and glucose. This study reveals the promise of using sodium thiosulphate along with selected substrate for enriching the quality and quantity of lipids, which can be valuable for exploiting algae as a source of biodiesel.
authors: Division of Microbiology, Indian Agricultural Research Institute (IARI), New Delhi, 110012 India.
authors: Momocha
authors: M
authors: Ngangkham
authors: Sachitra Kumar
authors: SK
authors: Ratha
authors: Radha
authors: R
authors: Prasanna
authors: Anil Kumar
authors: AK
authors: Saxena
authors: Dolly Wattal
authors: DW
authors: Dhar
authors: Chandragiri
authors: C
authors: Sarika
authors: Rachapudi Badari Narayana
authors: RB
authors: Prasad
keywords: Carbon metabolism; Chlorella; FAME; Glucose; Lipids; Reducing agent
references: Plant Physiol. 2010 Oct;154(2):1001-11
references: 20720172
references: Biotechnol Lett. 2009 Jul;31(7):1043-9
references: 19322523
references: Biotechnol Adv. 2012 Sep-Oct;30(5):989-1000
references: 21889585
references: Eukaryot Cell. 2010 Apr;9(4):486-501
references: 20139239
references: Trends Biotechnol. 2008 Mar;26(3):126-31
references: 18221809
references: Curr Top Cell Regul. 1974;8(0):139-95
references: 4154092
references: J Basic Microbiol. 2013 May;53(5):440-50
references: 22736510
references: J Biosci Bioeng. 2006 Mar;101(3):223-6
references: 16716922
references: Water Res. 2011 Jan;45(1):11-36
references: 20970155
references: Bioresour Technol. 2011 Jan;102(1):106-10
references: 20591657
references: Curr Top Cell Regul. 1983;22:143-76
references: 6135568
references: Appl Microbiol Biotechnol. 2009 Aug;84(2):281-91
references: 19330327
references: Appl Microbiol Biotechnol. 2011 Jul;91(1):31-46
references: 21567179
references: Bioresour Technol. 2007 Feb;98(3):560-4
references: 16782327
references: Bioresour Technol. 2011 Jan;102(1):135-41
references: 20638272
references: Appl Microbiol Biotechnol. 2003 Mar;61(1):40-3
references: 12658513
references: J Cell Biol. 1985 Mar;100(3):965-73
references: 3972906
references: Appl Biochem Biotechnol. 2010 Mar;160(6):1674-84
references: 19424668
references: Bioresour Technol. 2009 Sep;100(17):3921-6
references: 19362823
references: Proc Natl Acad Sci U S A. 2011 Apr 26;108(17):6899-904
references: 21482809
references: Appl Microbiol Biotechnol. 2011 Aug;91(3):835-44
references: 21698379
references: Biotechnol Bioeng. 1987 May;29(7):832-43
references: 18576528
references: Bioresour Technol. 2006 Apr;97(6):841-6
references: 15936938
references: J Biotechnol. 2009 Apr 20;141(1-2):31-41
references: 19428728
references: Crit Rev Food Sci Nutr. 1991;30(6):555-73
references: 1741951
references: Environ Health Perspect. 1976 Aug;16:139-46
references: 1017417
references: Plant Cell Physiol. 2012 Aug;53(8):1380-90
references: 22642988
references: Biotechnol Adv. 2007 May-Jun;25(3):294-306
references: 17350212