title: Imaging dynamics of CD11c⁺ cells and Foxp3⁺ cells in progressive autoimmune insulitis in the NOD mouse model of type 1 diabetes.
abstract: OBJECTIVE
  The aim of this study was to visualise the dynamics and interactions of the cells involved in autoimmune-driven inflammation in type 1 diabetes.
  
  
  METHODS
  We adopted the anterior chamber of the eye (ACE) transplantation model to perform non-invasive imaging of leucocytes infiltrating the endocrine pancreas during initiation and progression of insulitis in the NOD mouse. Individual, ACE-transplanted islets of Langerhans were longitudinally and repetitively imaged by stereomicroscopy and two-photon microscopy to follow fluorescently labelled leucocyte subsets.
  
  
  RESULTS
  We demonstrate that, in spite of the immune privileged status of the eye, the ACE-transplanted islets develop infiltration and beta cell destruction, recapitulating the autoimmune insulitis of the pancreas, and exemplify this by analysing reporter cell populations expressing green fluorescent protein under the Cd11c or Foxp3 promoters. We also provide evidence that differences in morphological appearance of subpopulations of infiltrating leucocytes can be correlated to their distinct dynamic behaviour.
  
  
  CONCLUSIONS
  Together, these findings demonstrate that the kinetics and dynamics of these key cellular components of autoimmune diabetes can be elucidated using this imaging platform for single cell resolution, non-invasive and repetitive monitoring of the individual islets of Langerhans during the natural development of autoimmune diabetes.
authors: ISIM-Immunology, Faculty of Health and Medical Sciences, Copenhagen University, Copenhagen, Denmark.
authors: Anja
authors: A
authors: Schmidt-Christensen
authors: Lisbeth
authors: L
authors: Hansen
authors: Erwin
authors: E
authors: Ilegems
authors: Nina
authors: N
authors: Fransén-Pettersson
authors: Ulf
authors: U
authors: Dahl
authors: Shashank
authors: S
authors: Gupta
authors: Asa
authors: A
authors: Larefalk
authors: Tine D
authors: TD
authors: Hannibal
authors: Alexander
authors: A
authors: Schulz
authors: Per-Olof
authors: PO
authors: Berggren
authors: Dan
authors: D
authors: Holmberg
mesh_terms: D000818:Animals; D000867:Anterior Chamber / Q000276:immunology / Q000473:pathology*; D001323:Autoantibodies / Q000097:blood; D015551:Autoimmunity; D039521:CD11c Antigen / Q000276:immunology*; D003921:Diabetes Mellitus, Experimental / Q000276:immunology / Q000473:pathology; D003922:Diabetes Mellitus, Type 1 / Q000276:immunology / Q000473:pathology*; D018450:Disease Progression; D005260:Female; D005434:Flow Cytometry; D051858:Forkhead Transcription Factors / Q000276:immunology*; D007249:Inflammation / Q000276:immunology / Q000473:pathology*; D007515:Islets of Langerhans / Q000276:immunology / Q000473:pathology*; D051379:Mice; D008810:Mice, Inbred C57BL; D016688:Mice, Inbred NOD; D008856:Microscopy, Fluorescence; D011236:Prediabetic State / Q000276:immunology / Q000473:pathology*
chemical_list: D001323:Autoantibodies; D039521:CD11c Antigen; D051858:Forkhead Transcription Factors; C498833:Foxp3 protein, mouse
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