title: Imidazole-induced contractility of vascular smooth muscle cells in the presence of U-73122, ODQ, indomethacin and 7-nitroindazole.
abstract: The aim of the study was to assess the impact of modulating factors on vascular smooth muscle cells reactivity. Vascular resistance was induced by the administration of increasing concentrations of imidazole. The experiments were performed on isolated and perfused tail artery of Wistar rats (weight 250 g - 350 g). Rats were been narcotized by urethane (intraperitoneal injection) at a dose of 120 mg/kg, stunned and then sacrificed by cervical dislocation. In the following investigation classical pharmacometric methods were used. Relationships between concentration-response curves (CRCs) for imidazole observed in the presence of ODQ [(1H-(1,2,4)oxadiazolo-[4,3-a]quinoxalin-1-one)], 7-nitroindazole and indomethacin were analyzed. Imidazole-induced contractility of vascular smooth muscle cells was independent from alpha-adrenergic receptors and PLC activity. Reactivity of VSMCsinduced by imidazole, was significantly changed in the presence of ODQ and 7-nitroindazole.
authors: Department of Pharmacology and Therapeutics, Nicolaus Copernicus University, Collegium Medicum in Bydgoszcz, M. Curie 9, 85-090 Bydgoszcz, Poland. wicinski4@wp.pl
authors: M
authors: M
authors: Wiciński
authors: G
authors: G
authors: Grześk
authors: B
authors: B
authors: Malinowski
authors: E
authors: E
authors: Grześk
authors: A T
authors: AT
authors: Sinjab
authors: M
authors: M
authors: Krzyzanowski
authors: A
authors: A
authors: Michalska
authors: D
authors: D
authors: Bylina
authors: J
authors: J
authors: Dziecioł
authors: K
authors: K
authors: Szadujkis-Szadurska
authors: A
authors: A
authors: Czeczuk
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004963:Estrenes / Q000494:pharmacology*; D006162:Guanylate Cyclase / Q000037:antagonists & inhibitors; D007093:Imidazoles / Q000494:pharmacology*; D007191:Indazoles / Q000494:pharmacology*; D007213:Indomethacin / Q000494:pharmacology*; D009131:Muscle, Smooth, Vascular / Q000166:cytology*; D032389:Myocytes, Smooth Muscle / Q000187:drug effects; D010069:Oxadiazoles / Q000494:pharmacology*; D010726:Phosphodiesterase Inhibitors / Q000494:pharmacology; D011760:Pyrrolidinones / Q000494:pharmacology*; D051381:Rats; D018160:Receptors, Cytoplasmic and Nuclear / Q000037:antagonists & inhibitors; D000071756:Soluble Guanylyl Cyclase; D014661:Vasoconstriction / Q000187:drug effects
chemical_list: C116907:1H-(1,2,4)oxadiazolo(4,3-a)-quiloxalin-1-one; D000894:Anti-Inflammatory Agents, Non-Steroidal; D004791:Enzyme Inhibitors; D004963:Estrenes; D007093:Imidazoles; D007191:Indazoles; D010069:Oxadiazoles; D010726:Phosphodiesterase Inhibitors; D011760:Pyrrolidinones; D018160:Receptors, Cytoplasmic and Nuclear; C060229:1-(6-((3-methoxyestra-1,3,5(10)-trien-17-yl)amino)hexyl)-1H-pyrrole-2,5-dione; C029899:imidazole; D006162:Guanylate Cyclase; D000071756:Soluble Guanylyl Cyclase; C080122:7-nitroindazole; D007213:Indomethacin