title: Effects of resveratrol on methotrexate-induced testicular damage in rats.
abstract: This study investigated the probable protective effects of resveratrol (RES), an antioxidant, against methotrexate- (MTX-) induced testis damage. Twenty-four male Sprague Dawley rats were randomly divided into four groups: control, RES, MTX, and MTX + RES groups. Rats were sacrificed at the end of the experiment. Plasma and tissue malondialdehyde (MDA) levels, superoxide dismutase (SOD) and catalase (CAT) activity in tissue, testicular histopathological damage scores, and testicular and epididymal epithelial apoptotic index (AI) were evaluated. The MTX group had significantly higher plasma and tissue MDA levels and significantly lower SOD and CAT activity than those of the control group. In the MTX + RES group, plasma and tissue MDA levels decreased significantly and SOD activity rose significantly compared to the MTX group. The MTX group had significantly lower Johnsen's testicular biopsy score (JTBS) values than those of the control group. JTBS was significantly higher in the MTX + RES group than in the MTX group. AI increased in the testis and epididymis in the MTX group and significantly decreased in the MTX + RES group. Our results indicate that RES has protective effects against MTX-induced testis damage at the biochemical, histopathological, and apoptotic levels.
authors: Department of Histology and Embryology, Faculty of Medicine, Karadeniz Technical University, 61080 Trabzon, Turkey. esinyulug@gmail.com
authors: Esin
authors: E
authors: Yuluğ
authors: Sibel
authors: S
authors: Türedi
authors: Ahmet
authors: A
authors: Alver
authors: Süleyman
authors: S
authors: Türedi
authors: Cemil
authors: C
authors: Kahraman
mesh_terms: D000818:Animals; D000964:Antimetabolites, Antineoplastic / Q000633:toxicity*; D000975:Antioxidants / Q000494:pharmacology*; D002374:Catalase / Q000378:metabolism; D020287:In Situ Nick-End Labeling; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D008727:Methotrexate / Q000633:toxicity*; D051381:Rats; D017207:Rats, Sprague-Dawley; D000077185:Resveratrol; D013267:Stilbenes / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000378:metabolism; D013737:Testis / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism
chemical_list: D000964:Antimetabolites, Antineoplastic; D000975:Antioxidants; D013267:Stilbenes; D008315:Malondialdehyde; D002374:Catalase; D013482:Superoxide Dismutase; D000077185:Resveratrol; D008727:Methotrexate
references: Ann Intern Med. 1980 Jul;93(1):109-14
references: 6994532
references: J Physiol Pharmacol. 2009 Oct;60 Suppl 4:111-6
references: 20083859
references: Asian J Androl. 2008 Mar;10(2):291-7
references: 18097501
references: Exp Toxicol Pathol. 2010 Mar;62(2):109-15
references: 19327973
references: J Am Coll Nutr. 1999 Oct;18(5):426-33
references: 10511324
references: Int J Urol. 2013 Nov;20(11):1130-5
references: 23418991
references: Anal Biochem. 1978 May;86(1):271-8
references: 655387
references: J Biol Chem. 1951 Nov;193(1):265-75
references: 14907713
references: Eur J Pharmacol. 2006 Aug 7;542(1-3):170-8
references: 16793036
references: Fertil Steril. 2009 Dec;92(6):2028-33
references: 19046577
references: J Pineal Res. 2005 Jan;38(1):1-9
references: 15617531
references: Hum Exp Toxicol. 2008 Jul;27(7):547-52
references: 18829730
references: Asian J Androl. 2003 Sep;5(3):231-42
references: 12937808
references: J Nutr. 2005 Apr;135(4):757-60
references: 15795430
references: ScientificWorldJournal. 2012;2012:987508
references: 22566785
references: Reprod Toxicol. 2001 Sep-Oct;15(5):479-86
references: 11780955
references: Mutat Res. 2009 Feb 19;673(1):43-52
references: 19110071
references: Arch Pharm Res. 2008 Jan;31(1):83-7
references: 18277612
references: Curr Med Chem. 2001 Jun;8(7):851-62
references: 11375755
references: Biochem Biophys Res Commun. 2003 Oct 3;309(4):1017-26
references: 13679076
references: J Pediatr Surg. 2012 Sep;47(9):1716-23
references: 22974612
references: Chin Med J (Engl). 2008 Jul 5;121(13):1204-9
references: 18710639
references: Mol Cell Biochem. 2004 Dec;267(1-2):209-14
references: 15663203
references: Hum Reprod. 1996 Aug;11(8):1655-60
references: 8921112
references: Br J Pharmacol. 2002 Apr;135(7):1627-33
references: 11934802
references: Andrologia. 2009 Apr;41(2):105-10
references: 19260847
references: Hormones. 1970;1(1):2-25
references: 5527187
references: Scand J Gastroenterol. 2004 Nov;39(11):1119-27
references: 15545171
references: Rev Reprod. 1997 Jan;2(1):48-54
references: 9414465
references: Eur J Pediatr Surg. 2005 Oct;15(5):333-6
references: 16254845
references: Hum Reprod. 2004 Jan;19(1):129-38
references: 14688171
references: Clin Chem. 1988 Mar;34(3):497-500
references: 3349599
references: Reprod Toxicol. 1999 Sep-Oct;13(5):391-7
references: 10560588