title: A modified semi-nested multiplex malaria PCR (SnM-PCR) for the identification of the five human Plasmodium species occurring in Southeast Asia.
abstract: We have modified an existing semi-nested multiplex polymerase chain reaction (PCR) by adding one Plasmodium knowlesi-specific nested PCR, and validated the latter against laboratory and clinical samples. This new method has the advantage of being relatively affordable in low resource settings while identifying the five human Plasmodium species with a three-step PCR.
authors: Nguyen
authors: N
authors: Van Hong
authors: Peter
authors: P
authors: van den Eede
authors: Chantal
authors: C
authors: Van Overmeir
authors: Indra
authors: I
authors: Vythilingham
authors: Anna
authors: A
authors: Rosanas-Urgell
authors: Pham
authors: P
authors: Vinh Thanh
authors: Ngo Duc
authors: ND
authors: Thang
authors: Nguyen Manh
authors: NM
authors: Hung
authors: Le Xuan
authors: LX
authors: Hung
authors: Umberto
authors: U
authors: D'Alessandro
authors: Annette
authors: A
authors: Erhart
mesh_terms: D000818:Animals; D001210:Asia, Southeastern / Q000453:epidemiology; D006801:Humans; D008288:Malaria / Q000453:epidemiology / Q000469:parasitology*; D010961:Plasmodium / Q000145:classification* / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*
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