title: Attachment of Acidithiobacillus ferrooxidans onto different solid substrates and fitting through Langmuir and Freundlich equations.
abstract: Attachments of Acidithiobacillus ferrooxidans ATCC 23270 onto elemental sulfur, quartz and complex chalcopyrite were investigated by analysis of its extracellular polymeric substances as well as applying Langmuir and Freundlich equations. The two equations fitted the adsorption equilibrium data with significant correlation coefficient over 0.9. This indicated that bacterial attachment is complicated and involves Langmuir and Freundlich characterizations. Sulfur-grown cells showed the highest affinity for the three solid substrates. The investigated complex chalcopyrite possessed a higher maximum adsorption capacity for A. ferrooxidans than elemental sulfur or quartz. The Freundlich fitting parameters suggested that quartz had a weaker adsorption capacity and smaller adsorption areas than elemental sulfur or the complex chalcopyrite. It is not the content of total carbohydrates or proteins in EPS but their ratios that determine the affinity differences between cells and substrates.
authors: Key Laboratory of Biohydrometallurgy of Ministry of Education, School of Minerals Processing and Bioengineering, Central South University, Changsha, 410083, Hunan, China, xialex888@163.com.
authors: Le-Xian
authors: LX
authors: Xia
authors: Zhen
authors: Z
authors: Shen
authors: Tomas
authors: T
authors: Vargas
authors: Wen-Juan
authors: WJ
authors: Sun
authors: Ren-Man
authors: RM
authors: Ruan
authors: Zhen-Da
authors: ZD
authors: Xie
authors: Guan-Zhou
authors: GZ
authors: Qiu
mesh_terms: D042763:Acidithiobacillus / Q000737:chemistry / Q000166:cytology / Q000378:metabolism*; D000327:Adsorption; D001422:Bacterial Adhesion / Q000502:physiology*; D003300:Copper / Q000378:metabolism*; D005110:Extracellular Space / Q000737:chemistry / Q000378:metabolism; D007700:Kinetics; D016014:Linear Models; D008962:Models, Theoretical; D011135:Polysaccharides, Bacterial / Q000737:chemistry / Q000378:metabolism; D011791:Quartz / Q000378:metabolism*; D013455:Sulfur / Q000378:metabolism*
chemical_list: D011135:Polysaccharides, Bacterial; C012819:chalcopyrite; D011791:Quartz; D013455:Sulfur; D003300:Copper