title: An odorant-binding protein required for suppression of sweet taste by bitter chemicals.
abstract: Animals often must decide whether or not to consume a diet that contains competing attractive and aversive compounds. Here, using the fruit fly, Drosophila melanogaster, we describe a mechanism that influences this decision. Addition of bitter compounds to sucrose suppressed feeding behavior, and this inhibition depended on an odorant-binding protein (OBP) termed OBP49a. In wild-type flies, bitter compounds suppressed sucrose-induced action potentials, and the inhibition was impaired in Obp49a mutants. However, loss of OBP49a did not affect action potentials in sugar- or bitter-activated gustatory receptor neurons (GRNs) when the GRNs were presented with just one type of tastant. OBP49a was expressed in accessory cells and acted non-cell-autonomously to attenuate nerve firings in sugar-activated GRNs when bitter compounds were combined with sucrose. These findings demonstrate an unexpected role for an OBP in taste and identify a molecular player involved in the integration of opposing attractive and aversive gustatory inputs.
authors: Department of Oral Biology, BK21 Project, Yonsei University College of Dentistry, 250 Seongsanno, Seodaemun-gu, Seoul 120-752, Korea.
authors: Yong Taek
authors: YT
authors: Jeong
authors: Jaewon
authors: J
authors: Shim
authors: So Ra
authors: SR
authors: Oh
authors: Hong In
authors: HI
authors: Yoon
authors: Chul Hoon
authors: CH
authors: Kim
authors: Seok Jun
authors: SJ
authors: Moon
authors: Craig
authors: C
authors: Montell
mesh_terms: D000200:Action Potentials / Q000187:drug effects / Q000502:physiology; D018712:Analgesics, Non-Narcotic / Q000494:pharmacology; D000818:Animals; D030801:Animals, Genetically Modified; D002755:Choice Behavior / Q000187:drug effects / Q000502:physiology; D004305:Dose-Response Relationship, Drug; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism; D004331:Drosophila melanogaster; D004566:Electrodes; D005518:Food Preferences / Q000187:drug effects / Q000502:physiology; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D009154:Mutation / Q000235:genetics; D009812:Odorants; D000644:Quaternary Ammonium Compounds / Q000494:pharmacology; D011803:Quinine / Q000494:pharmacology; D018035:Receptors, Odorant / Q000235:genetics* / Q000378:metabolism*; D058618:Sensilla / Q000187:drug effects / Q000502:physiology; D013395:Sucrose / Q000008:administration & dosage; D013549:Sweetening Agents / Q000494:pharmacology; D013649:Taste / Q000187:drug effects* / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
chemical_list: D018712:Analgesics, Non-Narcotic; D029721:Drosophila Proteins; D049452:Green Fluorescent Proteins; D000644:Quaternary Ammonium Compounds; D011803:Quinine; D018035:Receptors, Odorant; D013395:Sucrose; D013549:Sweetening Agents; D014157:Transcription Factors; C557617:GAL4 protein, Drosophila; C045648:odorant-binding protein; C043414:denatonium
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