title: MLST revisited: the gene-by-gene approach to bacterial genomics.
abstract: Multilocus sequence typing (MLST) was proposed in 1998 as a portable sequence-based method for identifying clonal relationships among bacteria. Today, in the whole-genome era of microbiology, the need for systematic, standardized descriptions of bacterial genotypic variation remains a priority. Here, to meet this need, we draw on the successes of MLST and 16S rRNA gene sequencing to propose a hierarchical gene-by-gene approach that reflects functional and evolutionary relationships and catalogues bacteria 'from domain to strain'. Our gene-based typing approach using online platforms such as the Bacterial Isolate Genome Sequence Database (BIGSdb) allows the scalable organization and analysis of whole-genome sequence data.
authors: Department of Zoology, University of Oxford, South Parks Road, Oxford OX1 3PS, UK.
authors: Martin C J
authors: MC
authors: Maiden
authors: Melissa J
authors: MJ
authors: Jansen van Rensburg
authors: James E
authors: JE
authors: Bray
authors: Sarah G
authors: SG
authors: Earle
authors: Suzanne A
authors: SA
authors: Ford
authors: Keith A
authors: KA
authors: Jolley
authors: Noel D
authors: ND
authors: McCarthy
mesh_terms: D000483:Alleles; D001419:Bacteria / Q000145:classification* / Q000235:genetics; D015373:Bacterial Typing Techniques / Q000379:methods; D001483:Base Sequence; D030541:Databases, Genetic; D019143:Evolution, Molecular; D014644:Genetic Variation; D016680:Genome, Bacterial; D023281:Genomics / Q000379:methods*; D058885:Multilocus Sequence Typing / Q000379:methods*; D012329:RNA, Bacterial / Q000032:analysis / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000032:analysis / Q000235:genetics
chemical_list: D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S
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