title: Gossypol suppresses mouse T lymphocytes via inhibition of NFκB, NFAT and AP-1 pathways.
abstract: Gossypol is a kind of yellow polyphenolic compounds extracted from root stem and seed of the cotton plant. In the present study, we investigated its immunosuppressive mechanism by using BALB/c mouse T lymphocytes in vitro. When mouse splenocytes was incubated with gossypol, the extract effectively suppress the overproduction of the cell stimulated by concanavalin A (ConA) in a dose manner. This inhibitive activity was mainly due to interfering Th1 and Th2 cytokines production and decreasing CD4(+) T cell populations and ratio of CD4(+)/CD8(+). Furthermore, we also showed that signal transduction via NF-κB, NFAT and AP-1 are critical to the ConA-induced T cell activation in mice. The data revealed that gossypol could down-regulate activation of ConA-induced NF-κB, NFAT and AP-1 signal transduction pathways in mouse T lymphocyte. These observations indicated that gossypol exhibited immunosuppressive effects by inhibition T lymphocyte activation in vitro.
authors: Key Laboratory of Zoonosis, Ministry of Education, College of Veterinary Medicine, Jilin University , Changchun, Jilin , People's Republic of China .
authors: Bocui
authors: B
authors: Song
authors: Guoren
authors: G
authors: Huang
authors: Chunyu
authors: C
authors: Tong
authors: Gen
authors: G
authors: Li
authors: Zhenning
authors: Z
authors: Wang
authors: Ying
authors: Y
authors: Xiong
authors: Shuang
authors: S
authors: Zhang
authors: Jing
authors: J
authors: Lu
authors: Shuang
authors: S
authors: Guan
mesh_terms: D000818:Animals; D003272:Contraceptive Agents, Male / Q000737:chemistry / Q000494:pharmacology*; D003368:Gossypium / Q000737:chemistry; D006072:Gossypol / Q000737:chemistry / Q000494:pharmacology*; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D016328:NF-kappa B / Q000276:immunology* / Q000378:metabolism; D050778:NFATC Transcription Factors / Q000276:immunology* / Q000378:metabolism; D018517:Plant Roots / Q000737:chemistry; D015398:Signal Transduction / Q000187:drug effects* / Q000276:immunology; D018417:Th1 Cells / Q000276:immunology* / Q000378:metabolism; D018418:Th2 Cells / Q000276:immunology* / Q000378:metabolism; D018808:Transcription Factor AP-1 / Q000276:immunology* / Q000378:metabolism
chemical_list: D003272:Contraceptive Agents, Male; D007166:Immunosuppressive Agents; D016328:NF-kappa B; D050778:NFATC Transcription Factors; D018808:Transcription Factor AP-1; D006072:Gossypol