title: Establishment of a simple living donor liver transplantation dog model using a two-step nonvenous bypass hepatectomy.
abstract: OBJECTIVE
  Dogs are an ideal model for studying living donor liver transplantation (LDLT). However, due to their poor tolerance to congestion and acidosis during portal vein blockage, current LDLT dog models with long operation times have high mortality. To address the issue, we designed a novel simplified operation with two-step nonvenous bypass (NVB) hepatectomy.
  
  
  METHODS
  Eighty dogs were evenly randomized to the living liver donor (LLD) or the recipient (LLR) groups. A standard lobectomy of I, II, and III lobes was performed in the LLD group. In the LLR group, first only I, II, and III lobes were resected using NVB; the residual lobes were resected off just after donor lobes were implanted.
  
  
  RESULTS
  For the LLD group, the operation time was 172.67 ± 20.98 minutes, amount of blood loss was 71.39 ± 13.59 mL, and 2-week survival rate was 85.00%. For the LLR group, the operation time was 251.61 ± 22.87 minutes, amount of blood loss was 220.00 ± 96.40 mL, amount of blood transfusion was 163.89 ± 44.74 mL, and 48-hour survival rate was 77.14%. In the LLR group, the mean arterial and central venous pressures decreased after organ implantion, but gradually recovered to normal levels after surgery. The liver function biochemical parameters recovered to preoperational levels after 14 days in the LLD group; in the LLR group, they gradually increased during 48 hours after operation.
  
  
  CONCLUSIONS
  The present method with two-step NVB hepatectomy can be used efficiently and safely for establishing LDLT dog model.
authors: Second Department of Hepatobiliary Surgery, Zhujiang Hospital, Southern Medical University, Guangzhou, China.
authors: M-X
authors: MX
authors: Pan
authors: L
authors: L
authors: Cai
authors: X-Y
authors: XY
authors: Wang
authors: Q-J
authors: QJ
authors: Zhang
authors: F-D
authors: FD
authors: Kong
authors: S-Y
authors: SY
authors: Xu
authors: Y
authors: Y
authors: Wang
authors: Y
authors: Y
authors: Gao
mesh_terms: D000818:Animals; D062186:Arterial Pressure; D015415:Biomarkers / Q000097:blood; D016063:Blood Loss, Surgical / Q000517:prevention & control; D001803:Blood Transfusion; D002496:Central Venous Pressure; D004285:Dogs; D005260:Female; D006498:Hepatectomy / Q000379:methods*; D008111:Liver Function Tests; D016031:Liver Transplantation / Q000379:methods*; D019520:Living Donors; D008297:Male; D023421:Models, Animal; D011183:Postoperative Complications / Q000097:blood / Q000503:physiopathology / Q000517:prevention & control; D020127:Recovery of Function; D013997:Time Factors
chemical_list: D015415:Biomarkers