title: Genome-wide analysis of transcription factor-binding sites in skeletal muscle cells using ChIP-seq.
abstract: Transcriptional regulation of gene expression constitutes a fundamental mechanism of many developmental processes. Therefore, identification and characterization of binding sites of transcription factors are important for uncovering the mechanisms of a particular developmental process. Here, we describe detailed procedures for genome-wide analysis of binding sites of a transcription factor involved in the fiber-type differentiation of skeletal muscle. By conducting ChIP-seq followed by a series of downstream analyses, in-depth information on binding sites of transcription factors can be obtained in a genome-wide manner.
authors: Division of Cardiovascular Medicine, Department of Internal Medicine, University of California, Davis, Davis, CA, USA.
authors: Chung-Ii
authors: CI
authors: An
authors: Nobuko
authors: N
authors: Hagiwara
mesh_terms: D000818:Animals; D001665:Binding Sites; D002454:Cell Differentiation; D047369:Chromatin Immunoprecipitation / Q000379:methods*; D005786:Gene Expression Regulation; D016678:Genome; D006801:Humans; D018485:Muscle Fibers, Skeletal / Q000166:cytology / Q000378:metabolism*; D018482:Muscle, Skeletal / Q000166:cytology*; D016133:Polymerase Chain Reaction / Q000379:methods; D011485:Protein Binding; D017422:Sequence Analysis, DNA / Q000379:methods; D014157:Transcription Factors / Q000378:metabolism*
chemical_list: D014157:Transcription Factors