title: Monocyte subset accumulation in the human heart following acute myocardial infarction and the role of the spleen as monocyte reservoir.
abstract: OBJECTIVE
  Monocytes are critical mediators of healing following acute myocardial infarction (AMI), making them an interesting target to improve myocardial repair. The purpose of this study was a gain of insight into the source and recruitment of monocytes following AMI in humans.
  
  
  RESULTS
  Post-mortem tissue specimens of myocardium, spleen and bone marrow were collected from 28 patients who died at different time points after AMI. Twelve patients who died from other causes served as controls. The presence and localization of monocytes (CD14(+) cells), and their CD14(+)CD16(-) and CD14(+)CD16(+) subsets, were evaluated by immunohistochemical and immunofluorescence analyses. CD14(+) cells localized at distinct regions of the infarcted myocardium in different phases of healing following AMI. In the inflammatory phase after AMI, CD14(+) cells were predominantly located in the infarct border zone, adjacent to cardiomyocytes, and consisted for 85% (78-92%) of CD14(+)CD16(-) cells. In contrast, in the subsequent post-AMI proliferative phase, massive accumulation of CD14(+) cells was observed in the infarct core, containing comparable proportions of both the CD14(+)CD16(-) [60% (31-67%)] and CD14(+)CD16(+) subsets [40% (33-69%)]. Importantly, in AMI patients, of the number of CD14(+) cells was decreased by 39% in the bone marrow and by 58% in the spleen, in comparison with control patients (P = 0.02 and <0.001, respectively).
  
  
  CONCLUSIONS
  Overall, this study showed a unique spatiotemporal pattern of monocyte accumulation in the human myocardium following AMI that coincides with a marked depletion of monocytes from the spleen, suggesting that the human spleen contains an important reservoir function for monocytes.
authors: Department of Cardiology, Academic Medical Centre, University of Amsterdam, Amsterdam, The Netherlands.
authors: Anja M
authors: AM
authors: van der Laan
authors: Ellis N
authors: EN
authors: Ter Horst
authors: Ronak
authors: R
authors: Delewi
authors: Mark P V
authors: MP
authors: Begieneman
authors: Paul A J
authors: PA
authors: Krijnen
authors: Alexander
authors: A
authors: Hirsch
authors: Mehrdad
authors: M
authors: Lavaei
authors: Matthias
authors: M
authors: Nahrendorf
authors: Anton J
authors: AJ
authors: Horrevoets
authors: Hans W M
authors: HW
authors: Niessen
authors: Jan J
authors: JJ
authors: Piek
mesh_terms: D000368:Aged; D015703:Antigens, CD / Q000378:metabolism; D001854:Bone Marrow Cells / Q000502:physiology; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D009000:Monocytes / Q000145:classification / Q000502:physiology*; D009203:Myocardial Infarction / Q000276:immunology / Q000473:pathology*; D009206:Myocardium / Q000473:pathology; D013154:Spleen / Q000276:immunology / Q000502:physiology*
chemical_list: D015703:Antigens, CD
keywords: Acute myocardial infarction; Bone marrow; Inflammation; Monocytes; Spleen
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