title: Structural studies of the O-specific polysaccharide(s) from the lipopolysaccharide of Azospirillum brasilense type strain Sp7.
abstract: Lipopolysaccharide was obtained by phenol-water extraction from dried bacterial cells of Azospirillum brasilense type strain Sp7. Mild acid hydrolysis of the lipopolysaccharide followed by GPC on Sephadex G-50 resulted in a polysaccharide mixture, which was studied by composition and methylation analyses, Smith degradation and (1)H and (13)C NMR spectroscopy. The following polysaccharide structures were established, where italics indicate a non-stoichiometric (∼40%) 2-O-methylation of l-rhamnose.
authors: Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, Prospekt Entuziastov 13, 410049 Saratov, Russia.
authors: Elena N
authors: EN
authors: Sigida
authors: Yuliya P
authors: YP
authors: Fedonenko
authors: Alexander S
authors: AS
authors: Shashkov
authors: Evelina L
authors: EL
authors: Zdorovenko
authors: Svetlana A
authors: SA
authors: Konnova
authors: Vladimir V
authors: VV
authors: Ignatov
authors: Yuriy A
authors: YA
authors: Knirel
mesh_terms: D015806:Azospirillum brasilense / Q000737:chemistry* / Q000145:classification; D002240:Carbohydrate Sequence; D008969:Molecular Sequence Data; D019081:O Antigens / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: D019081:O Antigens
keywords: Azospirillum brasilense; Bacterial polysaccharide structure; Lipopolysaccharide