title: Association of leptin receptor gene Q223R polymorphism on lipid profiles in comparison study between obese and non-obese subjects.
abstract: OBJECTIVE
  Leptin is a hormone secreted from adipocytes. It regulates metabolism and energy homeostasis through the leptin receptor (LEPR) which is localized centrally in hypothalamus as well as in peripheral tissues. The aim of this study was to investigate the association of leptin receptor gene Q223R polymorphism on obesity in association with body mass index (BMI), lipid parameters, plasma leptin levels and homeostasis model assessment of insulin resistance (HOMA-IR).
  
  
  METHODS
  The study included 110 obese and 90 non-obese subjects. The LEPR Q223R polymorphism was determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Plasma leptin levels, serum lipid and antropometric parameters were measured.
  
  
  RESULTS
  No association was found between LEPR gene Q223R polymorphism and BMI in both study and control groups. Strikingly study group with non-obese subjects and with the RR genotype (homozygous mutant) had significantly higher serum total cholesterol (p<0.001) and low density lipoprotein cholesterol (LDL-cholesterol) levels (p<0.05) than QR (heterozygous) and QQ (wild type) genotypes. In obese group, subjects with the RR genotypes had significantly higher triglycerides (p<0.05) levels, waist (p<0.05) and hip circumferences (p<0.001) than the QQ and QR genotypes.
  
  
  CONCLUSIONS
  Our results suggest that the LEPR gene Q223R polymorphism has an association with waist and hip circumferences in obese group but no direct association with obesity although there is a significant influence on lipid profile both in obese and non-obese subjects.
authors: Department of Biochemistry, Faculty of Pharmacy, Near East University, Nicosia, Mersin 10, Turkey. Electronic address: edabecer@yahoo.com.
authors: Eda
authors: E
authors: Becer
authors: Güldal
authors: G
authors: Mehmetçik
authors: Halin
authors: H
authors: Bareke
authors: Nedime
authors: N
authors: Serakıncı
mesh_terms: D000328:Adult; D001786:Blood Glucose / Q000032:analysis; D015992:Body Mass Index; D016022:Case-Control Studies; D008076:Cholesterol, HDL / Q000097:blood; D008078:Cholesterol, LDL / Q000097:blood; D005215:Fasting; D005260:Female; D005838:Genotype; D006801:Humans; D006949:Hyperlipidemias / Q000097:blood / Q000150:complications; D007333:Insulin Resistance; D020738:Leptin / Q000097:blood; D008297:Male; D008875:Middle Aged; D009765:Obesity / Q000097:blood / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D011446:Prospective Studies; D054411:Receptors, Leptin / Q000235:genetics* / Q000378:metabolism; D014280:Triglycerides / Q000097:blood; D055105:Waist Circumference
chemical_list: D001786:Blood Glucose; D008076:Cholesterol, HDL; D008078:Cholesterol, LDL; C515448:LEPR protein, human; D020738:Leptin; D054411:Receptors, Leptin; D014280:Triglycerides
keywords: 3-hydroxy-3-methylglutaryl coenzyme reductase; ANOVA; ApoB; BMI; Body mass index; ELISA; HDL-C; HMG-CoA reductase; HOMA-IR; LDL-C; LEPR; Leptin; Leptin receptor; PCR–RFLP; Polymorphism; SNPs; TG; VLDL; analysis of variance; apolipoprotein B; body mass index; enzyme-linked immunosorbent assay; high-density lipoprotein cholesterol; homeostasis model assessment of insulin resistance; leptin receptor; low density lipoprotein cholesterol; polymerase chain reaction–restriction fragment length polymorphism; single nucleotide polymorphisms; triglycerides; very-low density lipoprotein