title: Solution NMR structures provide first structural coverage of the large protein domain family PF08369 and complementary structural coverage of dark operative protochlorophyllide oxidoreductase complexes.
abstract: High-quality NMR structures of the C-terminal domain comprising residues 484-537 of the 537-residue protein Bacterial chlorophyll subunit B (BchB) from Chlorobium tepidum and residues 9-61 of 61-residue Asr4154 from Nostoc sp. (strain PCC 7120) exhibit a mixed α/β fold comprised of three α-helices and a small β-sheet packed against second α-helix. These two proteins share 29% sequence similarity and their structures are globally quite similar. The structures of BchB(484-537) and Asr4154(9-61) are the first representative structures for the large protein family (Pfam) PF08369, a family of unknown function currently containing 610 members in bacteria and eukaryotes. Furthermore, BchB(484-537) complements the structural coverage of the dark-operating protochlorophyllide oxidoreductase.
authors: Department of Chemistry, The State University of New York at Buffalo, Buffalo, NY 14260, USA.
authors: Surya V S R K
authors: SV
authors: Pulavarti
authors: Yunfen
authors: Y
authors: He
authors: Erik A
authors: EA
authors: Feldmann
authors: Alexander
authors: A
authors: Eletsky
authors: Thomas B
authors: TB
authors: Acton
authors: Rong
authors: R
authors: Xiao
authors: John K
authors: JK
authors: Everett
authors: Gaetano T
authors: GT
authors: Montelione
authors: Michael A
authors: MA
authors: Kennedy
authors: Thomas
authors: T
authors: Szyperski
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry / Q000648:ultrastructure; D041883:Chlorobium / Q000737:chemistry; D061146:Chlorophyll Binding Proteins / Q000737:chemistry / Q000648:ultrastructure*; D046937:Nostoc / Q000737:chemistry; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D010088:Oxidoreductases / Q000737:chemistry / Q000648:ultrastructure*; D011521:Protochlorophyllide / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D061146:Chlorophyll Binding Proteins; D011521:Protochlorophyllide; D010088:Oxidoreductases
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