title: High sensitive and selective HPTLC method assisted by digital image processing for simultaneous determination of catecholamines and related drugs.
abstract: A highly sensitive and selective thin layer chromatographic (TLC) method was developed for simultaneous determination of catecholamines and their related drugs using a new detection method and digital image processing of chromatographic plates. For the quantitative evaluation of the investigated compounds, the chromatographic separation was followed by spraying the plate with 0.02% solution of 2,2-diphenyl-1-picrylhydrazyl radical (DPPH) in ethanol. The BioDit Thin Layer Chromatography (TLC) Scanner device and advanced specific software (ImageDecipher-TLC, Sorbfil TLC Videodensitometer and JustTLC) were used for the detection and quantification of chromatographic spots. For an accurate determination, the RGB colored images of the bright-white spots detected against a purple background were inverted and processed after their conversion into green scale. The results showed a strongly linear correlation between area (R(2)>0.99) and volume (R(2)>0.99) of spots and concentration of investigated compounds in all cases. The limit of detection (LOD) and the limit of quantification (LOQ) were below 49.3 ng/spot and 69.6 ng/spot respectively in all cases. The evaluation of the method was performed using different pharmaceutical samples spiked with the investigated amines and validated with respect to accuracy and precision.
authors: Faculty of Chemistry and Chemical Engineering, Babeş-Bolyai University, Arany Janos Str., No 11, RO-400028, Cluj-Napoca, România.
authors: Ioana Anamaria
authors: IA
authors: Sima Tuhuţiu
authors: Dorina
authors: D
authors: Casoni
authors: Costel
authors: C
authors: Sârbu
mesh_terms: D001713:Biphenyl Compounds / Q000737:chemistry; D002395:Catecholamines / Q000032:analysis* / Q000737:chemistry; D002855:Chromatography, Thin Layer / Q000379:methods; D007091:Image Processing, Computer-Assisted; D007545:Isoproterenol / Q000032:analysis* / Q000737:chemistry; D008750:Methyldopa / Q000032:analysis* / Q000737:chemistry; D010851:Picrates / Q000737:chemistry; D015203:Reproducibility of Results
chemical_list: D001713:Biphenyl Compounds; D002395:Catecholamines; D010851:Picrates; D008750:Methyldopa; C004931:1,1-diphenyl-2-picrylhydrazyl; D007545:Isoproterenol
keywords: Catecholamines; DPPH free radical; Digital image processing; RP-HPTLC