title: Separation and purification of water-soluble iridoid glucosides by high speed counter-current chromatography combined with macroporous resin column separation.
abstract: Four iridoid glucosides, shanzhiside methyl ester, phloyoside II, chlorotuberside, and penstemonoside, were isolated and purified from an herbal medicinal plant for the first time by high-speed counter-current chromatography (HSCCC) using a two-phase solvent system composed of ethyl acetate-n-butanol-water (5:14:12, v/v/v). A total of 37mg of shanzhiside methyl ester, 29mg of phloyoside II, 27mg of chlorotuberside, and 21mg of penstemonoside with the purity of 99.2%, 98.5%, 97.3%, and 99.3%, respectively, were obtained in one-step separation within 4h from 150mg of crude extract. To the best of our knowledge, this is the first report of separation and purification of iridoid glucosides from natural sources by HSCCC. The chemical structures of all the four compounds were identified by ESI-MS, (1)H NMR, and (13)C NMR.
authors: Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810001, China.
authors: Hui-Lan
authors: HL
authors: Yue
authors: Xiao-Hui
authors: XH
authors: Zhao
authors: Qi-Lan
authors: QL
authors: Wang
authors: Yan-Duo
authors: YD
authors: Tao
mesh_terms: D000440:Butanols / Q000737:chemistry; D003377:Countercurrent Distribution / Q000379:methods*; D000431:Ethanol / Q000737:chemistry; D057888:Iridoid Glucosides / Q000032:analysis / Q000302:isolation & purification*; D019686:Lamiaceae / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry*; D012997:Solvents / Q000737:chemistry
chemical_list: D000440:Butanols; D057888:Iridoid Glucosides; D010936:Plant Extracts; D012997:Solvents; D000431:Ethanol
keywords: Chlorotuberside; Lamiophlomis rotata (Benth.) Kudo; Penstemonoside; Phloyoside II; Shanzhiside methyl ester