title: (S)-[6]-Gingerol enhances glucose uptake in L6 myotubes by activation of AMPK in response to [Ca2+]i.
abstract: OBJECTIVE
  The aim of this study was to investigate the mechanism of (S)-[6]-gingerol in promoting glucose uptake in L6 skeletal muscle cells.
  
  
  METHODS
  The effect of (S)-[6]-gingerol on glucose uptake in L6 myotubes was examined using 2-[1,2-3H]-deoxy-D-glucose. Intracellular Ca2+ concentration was measured using Fluo-4. Phosphorylation of AMPKα was determined by Western blotting analysis.
  
  
  RESULTS
  (S)-[6]-Gingerol time-dependently enhanced glucose uptake in L6 myotubes. (S)-[6]-Gingerol elevated intracellular Ca2+ concentration and subsequently induced a dose- and time-dependent enhancement of threonine172 phosphorylated AMPKα in L6 myotubes via modulation by Ca2+/calmodulin-dependent protein kinase kinase.
  
  
  CONCLUSIONS
  The results indicated that (S)-[6]-gingerol increased glucose uptake in L6 skeletal muscle cells by activating AMPK. (S)-[6]-gingerol, a major component of Zingiber officinale, may have potential for development as an antidiabetic agent.
authors: Faculty of Pharmacy, The University of Sydney, NSW, Australia.
authors: Yiming
authors: Y
authors: Li
authors: Van H
authors: VH
authors: Tran
authors: Nooshin
authors: N
authors: Koolaji
authors: Colin
authors: C
authors: Duke
authors: Basil D
authors: BD
authors: Roufogalis
mesh_terms: D055372:AMP-Activated Protein Kinases / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D002118:Calcium / Q000378:metabolism; D002396:Catechols / Q000494:pharmacology*; D002460:Cell Line; D005233:Fatty Alcohols / Q000494:pharmacology*; D005947:Glucose / Q000378:metabolism*; D007004:Hypoglycemic Agents / Q000494:pharmacology*; D018485:Muscle Fibers, Skeletal / Q000187:drug effects* / Q000378:metabolism; D034741:RNA, Small Interfering / Q000235:genetics; D051381:Rats
chemical_list: D002396:Catechols; D005233:Fatty Alcohols; D007004:Hypoglycemic Agents; D034741:RNA, Small Interfering; C007845:gingerol; C543928:Prkaa2 protein, rat; D055372:AMP-Activated Protein Kinases; C568305:Prkaa1 protein, rat; D005947:Glucose; D002118:Calcium