title: Differential diagnosis of Moniezia benedeni and M. expansa (Anoplocephalidae) by PCR using markers in small ribosomal DNA (18S rDNA).
abstract: Moniezia benedeni and M. expansa are common ruminant tapeworms of worldwide distribution, causing gastrointestinal disorders and even death in sheep and goats. In this study, a polymerase chain reaction- (PCR-) based approach for precise species identification was developed and also applied to faecal DNA diagnosis of the tapeworm infection. Since nuclear ribosomal DNA (rDNA) appears to be a useful target for species and/or strain markers, the 18S regions of the rDNA of M. benedeni and M. expansa were amplified and sequenced. The lengths and GC contents of the regions sequenced were 2476-2487 bp and 51.9-52.1% for M. benedeni and 2473 bp and 51.9-52.0% for M. expansa, respectively. Alignment and comparison of the 18S sequences of the two species showed 92.5-93.3% homology. No matches for the 18S regions of M. benedeni and M. expansa were found with other species by BLAST search, which made the 18S sequences appropriate markers for the design of distinctive primers for the two Moniezia species. Our 18S-based PCR could detect as low levels as 0.5 pg genomic DNA or the DNA extracted from 0.2 g faecal sample collected from the rectum of an M. expansa-infected goat. The results indicate that this PCR approach is a reliable alternative for the differential diagnosis of Moniezia species in faecal samples.
authors: CAAS State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Key Laboratory of Zoonoses of the Ministry of Agriculture, Lanzhou Veterinary Research Institute Lanzhou, Gansu Province 730046 The People's Republic of China.
authors: Hongbin
authors: H
authors: Yan
authors: Xinwen
authors: X
authors: Bo
authors: Youyu
authors: Y
authors: Liu
authors: Zhongzi
authors: Z
authors: Lou
authors: Xingwei
authors: X
authors: Ni
authors: Wangui
authors: W
authors: Shi
authors: Fang
authors: F
authors: Zhan
authors: Hongkean
authors: H
authors: Ooi
authors: Wanzhong
authors: W
authors: Jia
mesh_terms: D000818:Animals; D001483:Base Sequence; D002589:Cestoda; D002590:Cestode Infections; D004275:DNA, Ribosomal; D003937:Diagnosis, Differential; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012337:RNA, Ribosomal, 18S
chemical_list: D004275:DNA, Ribosomal; D012337:RNA, Ribosomal, 18S
keywords: Moniezia; PCR; diagnosis; ribosomal DNA (18S); ruminant