title: Neolignans from the fruits of Magnolia obovata and their inhibition effect on NO production in LPS-induced RAW 264.7 cells.
abstract: Three new neolignans, named 9-methoxyobovatol (6), magnobovatol (7), and 2-hydroxyobovaaldehyde (9), along with six known ones, magnolol (1), honokiol (2), isomagnolol (3), obovatol (4), obovatal (5), and obovaaldehyde (8), were isolated from the fruits of Magnolia obovata using silica gel and ODS column chromatography. From the results of spectroscopic data including EIMS, IR, 1H- and 13C-NMR, DEPT, and 2D-NMR (gCOSY, gHSQC, gHMBC), the chemical structures were determined. All isolated compounds were evaluated for inhibition activity on nitric oxide production in LPS-induced RAW 264.7 cells, and compounds 1-4, 6, 7, and 9 showed significant activity with IC50 values of 15.8 ± 0.3, 3.3 ± 1.2, 14.1 ± 0.9, 6.2 ± 1.2, 14.8 ± 2.3, 14.2 ± 1.2, and 14.8 ± 3.2 µM, respectively, without any visible toxic effect.
authors: Graduate School of Biotechnology and Department of Oriental Medicinal Materials and Processing, Kyung Hee University, Yongin, Republic of Korea.
authors: Kyeong-Hwa
authors: KH
authors: Seo
authors: Dae-Young
authors: DY
authors: Lee
authors: Dong-Sung
authors: DS
authors: Lee
authors: Ji-Hae
authors: JH
authors: Park
authors: Rak-Hun
authors: RH
authors: Jeong
authors: Ye-Jin
authors: YJ
authors: Jung
authors: Sabina
authors: S
authors: Shrestha
authors: In-Sik
authors: IS
authors: Chung
authors: Geum-Soog
authors: GS
authors: Kim
authors: Youn-Chul
authors: YC
authors: Kim
authors: Nam-In
authors: NI
authors: Baek
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D002460:Cell Line; D005638:Fruit / Q000737:chemistry; D007249:Inflammation / Q000139:chemically induced / Q000378:metabolism; D017705:Lignans / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D008070:Lipopolysaccharides; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D031566:Magnolia / Q000737:chemistry*; D051379:Mice; D015394:Molecular Structure; D009569:Nitric Oxide / Q000096:biosynthesis*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*
chemical_list: C587650:2-hydroxyobovaaldehyde; C587652:9-methoxyobovatol; D000893:Anti-Inflammatory Agents; D017705:Lignans; D008070:Lipopolysaccharides; D010936:Plant Extracts; C587653:magnobovatol; D009569:Nitric Oxide