title: Comparison of efficacy of Salmonella typhimurium A1-R and chemotherapy on stem-like and non-stem human pancreatic cancer cells.
abstract: The XPA1 human pancreatic cancer cell line is dimorphic, with spindle stem-like cells and round non-stem cells. We report here the in vitro IC 50 values of stem-like and non-stem XPA1 human pancreatic cells cells for: (1) 5-fluorouracil (5-FU), (2) cisplatinum (CDDP), (3) gemcitabine (GEM), and (4) tumor-targeting Salmonella typhimurium A1-R (A1-R). IC 50 values of stem-like XPA1 cells were significantly higher than those of non-stem XPA1 cells for 5-FU (P = 0.007) and CDDP (P = 0.012). In contrast, there was no difference between the efficacy of A1-R on stem-like and non-stem XPA1 cells. In vivo, 5-FU and A1-R significantly reduced the tumor weight of non-stem XPA1 cells (5-FU; P = 0.028; A1-R; P = 0.011). In contrast, only A1-R significantly reduced tumor weight of stem-like XPA1 cells (P = 0.012). The combination A1-R with 5-FU improved the antitumor efficacy compared with 5-FU monotherapy on the stem-like cells (P = 0.004). The results of the present report indicate A1-R is a promising therapy for chemo-resistant pancreatic cancer stem-like cells.
authors: AntiCancer, Inc; San Diego, CA USA; Department of Surgery; University of California San Diego; San Diego, CA USA; Yokohama City University Graduate School of Medicine; Yokohama, Japan.
authors: Yukihiko
authors: Y
authors: Hiroshima
authors: Ming
authors: M
authors: Zhao
authors: Yong
authors: Y
authors: Zhang
authors: Ali
authors: A
authors: Maawy
authors: Mohamed K
authors: MK
authors: Hassanein
authors: Fuminari
authors: F
authors: Uehara
authors: Shinji
authors: S
authors: Miwa
authors: Shuya
authors: S
authors: Yano
authors: Masashi
authors: M
authors: Momiyama
authors: Atsushi
authors: A
authors: Suetsugu
authors: Takashi
authors: T
authors: Chishima
authors: Kuniya
authors: K
authors: Tanaka
authors: Michael
authors: M
authors: Bouvet
authors: Itaru
authors: I
authors: Endo
authors: Robert M
authors: RM
authors: Hoffman
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology / Q000627:therapeutic use*; D000971:Antineoplastic Combined Chemotherapy Protocols / Q000494:pharmacology / Q000627:therapeutic use; D045744:Cell Line, Tumor; D019008:Drug Resistance, Neoplasm / Q000187:drug effects; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D018613:Microscopy, Confocal; D014411:Neoplastic Stem Cells / Q000187:drug effects / Q000473:pathology*; D010190:Pancreatic Neoplasms / Q000188:drug therapy* / Q000473:pathology*; D012486:Salmonella typhimurium / Q000378:metabolism*; D016896:Treatment Outcome; D023041:Xenograft Model Antitumor Assays
chemical_list: D000970:Antineoplastic Agents
keywords: GFP; RFP; Salmonella typhimurium; amino acid auxotrophy; chemoresistance; confocal microscopy; fluorescence imaging; pancreatic cancer; selective tumor targeting; stem cell
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