title: Production of Blastospore of Entomopathogenic Beauveria bassiana in a Submerged Batch Culture.
abstract: The principal objective of this study was to determine the optimal liquid culture conditions in shake flasks for maximal sporulation of Beauveria bassiana. The optimal initial pH for the spore production of B. bassiana using Potato Dextrose Broth was 5.2. The screening in shake flasks of carbon and nitrogen sources resulted in the identification of an optimal medium based on 3% sucrose and 1% casamino acid, with a C : N ratio of 22 : 4. Using this medium, a production level of 5.65 × 10(7) spores per ml was obtained after 5 days of culture. Using 3% corn meal, 2% corn steep powder, and 2% rice bran, the maximum spore concentration of 8.54 × 10(8)/ml was achieved 8 days after inoculation at 25℃ in a rotary shaking incubator operated at 200 rpm. This represents a yield gain of approximately 2.89 times that of pre-optimization.
authors: Department of Bioscience and Biotechnology, The University of Suwon, Hwaseong 445-743, Korea.
authors: Tuan Anh
authors: TA
authors: Pham
authors: Jeong Jun
authors: JJ
authors: Kim
authors: Seon Gon
authors: SG
authors: Kim
authors: Keun
authors: K
authors: Kim
keywords: Beauveria bassiana; Blastospore production; Liquid medium; Optimal culture conditions
references: Bacteriol Rev. 1948 Dec;12(4):297-311
references: 16350125
references: J Invertebr Pathol. 1999 Nov;74(3):261-6
references: 10534413
references: Appl Environ Microbiol. 1990 Nov;56(11):3435-8
references: 16348348
references: Mycobiology. 2005 Dec;33(4):215-22
references: 24049504
references: J Microbiol Biotechnol. 2009 Dec;19(12):1603-11
references: 20075626