title: Chicken embryonic stem cells: establishment and characterization.
abstract: Embryonic stem (ES) cells are unique models for investigating early development and cell differentiation. First identified in mouse and later in other mammals, these cells have also been isolated in avian species. Here, using chicken as a model, we describe a set of protocols allowing the isolation, maintenance, genetic modification, differentiation, and injection of the chicken embryonic stem (cES) cells into embryos for obtaining chimeric animals.
authors: Inserm, U846, Stem Cell and Brain Research Institute, Bron, France.
authors: Pauline
authors: P
authors: Aubel
authors: Bertrand
authors: B
authors: Pain
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation; D002645:Chickens; D053595:Embryonic Stem Cells / Q000166:cytology*