title: Angiogenesis enhanced by Phyllocaulis boraceiensis mucus in human cells.
abstract: Phyllocaulis boraceiensis mucus is known to be a compound capable of inducing cell proliferation and enhancing the wound healing process. The process of angiogenesis is a chain of mechanisms responsible for the formation of new vessels, which are are involved in cell proliferation, and factors that will act in the healing process. Our aim was to demonstrate that the angiogenesis process is enhanced in cultures of endothelial cells and fibroblasts treated with P. boraceiensis mucus. Experiments were carried out with 10(5) cells·mL(-1) of endothelial cells and fibroblasts treated with P. boraceiensis mucus in concentrations that have significant effects in proliferation assays, i.e. 0.012 μg·μL(-1) and 0.18 μg·μL(-1) , both of which cause extreme responses. Aliquots of 100 μL of cell suspensions were incubated for 1 h at 4 °C with 1 μL of antibodies specific for the cell markers vascular endothelial growth factor receptor 1 and cluster of differentiation 34, and negative isotype controls. Reading and expression analysis of cell markers was performed on a FACSCalibur flow cytometer. Expression levels of vascular endothelial growth factor receptor 1 and cluster of differentiation 34 expression were significantly increased in endothelial cells cultivated with 0.012 μg·μL(-1) P. boraceiensis mucus, suggesting that this compound is capable of enhancing angiogenesis.
authors: Laboratory of Biochemistry and Biophysics, Butantan Institute, São Paulo, Brazil.
authors: Ana R
authors: AR
authors: de Toledo-Piza
authors: Durvanei A
authors: DA
authors: Maria
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D005347:Fibroblasts / Q000166:cytology; D005434:Flow Cytometry; D049851:Gastropoda; D006801:Humans; D009093:Mucus; D018919:Neovascularization, Physiologic; D012867:Skin / Q000166:cytology
keywords: CD34; Phyllocaulis boraceiensis mucus; angiogenesis; molluscs; proliferation; vascular endothelial growth factor (VEGF)