title: In situ fabrication of silver nanoparticle-filled hydrogen titanate nanotube layer on metallic titanium surface for bacteriostatic and biocompatible implantation.
abstract: A silver nanoparticle (AgNP)-filled hydrogen titanate nanotube layer was synthesized in situ on a metallic titanium substrate. In the synthesis approach, a layer of sodium titanate nanotubes is first prepared on the titanium surface by using a hydrothermal method. Silver nitrate solution is absorbed into the nanotube channels by immersing a dried nanotube layer in silver nitrate solution. Finally, silver ions are reduced by glucose, leading to the in situ growth of AgNPs in the hydrogen titanate nanotube channels. Long-term silver release and bactericidal experiments demonstrated that the effective silver release and effective antibacterial period of the titanium foil with a AgNP-filled hydrogen titanate nanotube layer on the surface can extend to more than 15 days. This steady and prolonged release characteristic is helpful to promote a long-lasting antibacterial capability for the prevention of severe infection after surgery. A series of antimicrobial and biocompatible tests have shown that the sandwich nanostructure with a low level of silver loading exhibits a bacteriostatic rate as high as 99.99%, while retaining low toxicity for cells and possessing high osteogenic potential. Titanium foil with a AgNP-filled hydrogen titanate nanotube layer on the surface that is fabricated with low-cost surface modification methods is a promising implantable material that will find applications in artificial bones, joints, and dental implants.
authors: Department of Cardiology, Heilongjiang Provincial Hospital, Haerbin, Heilongjiang, People's Republic of China.
authors: Zheng
authors: Z
authors: Wang
authors: Yan
authors: Y
authors: Sun
authors: Dongzhou
authors: D
authors: Wang
authors: Hong
authors: H
authors: Liu
authors: Robert I
authors: RI
authors: Boughton
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000704:Analysis of Variance; D000818:Animals; D000900:Anti-Bacterial Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D004926:Escherichia coli / Q000187:drug effects; D006859:Hydrogen / Q000737:chemistry; D008422:Materials Testing; D053768:Metal Nanoparticles / Q000737:chemistry*; D008856:Microscopy, Fluorescence; D043942:Nanotubes / Q000737:chemistry*; D010087:Oxides / Q000737:chemistry*; D019736:Prostheses and Implants; D012834:Silver / Q000737:chemistry* / Q000494:pharmacology; D013059:Spectrum Analysis, Raman; D014025:Titanium / Q000737:chemistry*
chemical_list: D000900:Anti-Bacterial Agents; D010087:Oxides; C471701:sodium titanate; D012834:Silver; D006859:Hydrogen; D014025:Titanium; D000469:Alkaline Phosphatase
keywords: bacteriostasis; cytocompatibility; ion substitution; silver nanoparticle filling; titanium implant
references: Biomaterials. 2003 Sep;24(21):3725-30
references: 12818544
references: Lancet. 2001 Jul 14;358(9276):135-8
references: 11463434
references: Infection. 1999;27 Suppl 1:S46-8
references: 10379444
references: Biomaterials. 2005 Aug;26(24):4938-43
references: 15769528
references: ACS Nano. 2009 Feb 24;3(2):279-90
references: 19236062
references: Biomaterials. 2012 Jul;33(21):5267-77
references: 22541354
references: Biomaterials. 2006 Nov;27(32):5512-7
references: 16872671
references: Clin Infect Dis. 1999 Dec;29(6):1371-7; quiz 1378
references: 10585781
references: J Phys Chem B. 2005 Apr 7;109(13):6210-4
references: 16851687
references: J Mater Sci Mater Med. 1999 Jan;10(1):35-46
references: 15347992
references: Science. 1985 May 24;228(4702):990-3
references: 4001933
references: J R Soc Interface. 2008 Oct 6;5(27):1137-58
references: 18667387
references: Water Res. 2008 Jun;42(12):3066-74
references: 18359055
references: Proc Natl Acad Sci U S A. 2005 Apr 26;102(17):5953-7
references: 15827122
references: J Am Chem Soc. 2006 Aug 2;128(30):9798-808
references: 16866536
references: N Engl J Med. 2004 Oct 14;351(16):1645-54
references: 15483283
references: Wiley Interdiscip Rev Nanomed Nanobiotechnol. 2010 Nov-Dec;2(6):670-84
references: 20730806
references: Nanotechnology. 2005 Oct;16(10):2346-53
references: 20818017
references: J Biomed Mater Res B Appl Biomater. 2009 Oct;91(1):470-80
references: 19637369
references: Chemistry. 2003 May 23;9(10):2229-38
references: 12772297
references: Clin Orthop Relat Res. 1992 Jul;(280):200-7
references: 1611745
references: Int J Nanomedicine. 2010 May 13;5:337-42
references: 20517478
references: Biomaterials. 2005 May;26(14):2081-8
references: 15576182
references: J Biomed Mater Res B Appl Biomater. 2011 May;97(2):299-305
references: 21394901
references: Biomaterials. 2010 Feb;31(4):680-90
references: 19864019
references: J Nanosci Nanotechnol. 2007 Feb;7(2):668-72
references: 17450812
references: J Biomed Mater Res B Appl Biomater. 2010 Nov;95(2):441-8
references: 20878930
references: Calcif Tissue Int. 1982 Jan;34(1):76-9
references: 6802463
references: J Bone Joint Surg Am. 1995 Oct;77(10):1576-88
references: 7593069
references: J Bone Joint Surg Am. 1988 Jun;70(5):724-7
references: 3292531
references: Biomed Eng Online. 2006 Mar 24;5:22
references: 16556327
references: Biomaterials. 2011 Jan;32(3):693-705
references: 20970183
references: Biotechnol Adv. 2009 Jan-Feb;27(1):76-83
references: 18854209