title: Administration of murine stromal vascular fraction ameliorates chronic experimental autoimmune encephalomyelitis.
abstract: Administration of adipose-derived stromal/stem cells (ASCs) represents a promising therapeutic approach for autoimmune diseases since they have been shown to have immunomodulatory properties. The uncultured, nonexpanded counterpart of ASCs, the stromal vascular fraction (SVF), is composed of a heterogeneous mixture of cells. Although administration of ex vivo culture-expanded ASCs has been used to study immunomodulatory mechanisms in multiple models of autoimmune diseases, less is known about SVF-based therapy. The ability of murine SVF cells to treat myelin oligodendrocyte glycoprotein35-55-induced experimental autoimmune encephalitis (EAE) was compared with that of culture-expanded ASCs in C57Bl/6J mice. A total of 1 × 10(6) SVF cells or ASCs were administered intraperitoneally concomitantly with the induction of disease. The data indicate that intraperitoneal administration of ASCs significantly ameliorated the severity of disease course. They also demonstrate, for the first time, that the SVF effectively inhibited disease severity and was statistically more effective than ASCs. Both cell therapies also demonstrated a reduction in tissue damage, a decrease in inflammatory infiltrates, and a reduction in sera levels of interferon-γ and interleukin-12. Based on these data, SVF cells effectively inhibited EAE disease progression more than culture-expanded ASCs.
authors: Center for Stem Cell Research and Regenerative Medicine and.
authors: Julie A
authors: JA
authors: Semon
authors: Xiujuan
authors: X
authors: Zhang
authors: Amitabh C
authors: AC
authors: Pandey
authors: Sandra M
authors: SM
authors: Alandete
authors: Catherine
authors: C
authors: Maness
authors: Shijia
authors: S
authors: Zhang
authors: Brittni A
authors: BA
authors: Scruggs
authors: Amy L
authors: AL
authors: Strong
authors: Steven A
authors: SA
authors: Sharkey
authors: Marc M
authors: MM
authors: Beuttler
authors: Jeffrey M
authors: JM
authors: Gimble
authors: Bruce A
authors: BA
authors: Bunnell
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000473:pathology / Q000628:therapy*; D005434:Flow Cytometry; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D033581:Stem Cell Transplantation; D017154:Stromal Cells / Q000637:transplantation*
keywords: Adipose; Adult stem cells; Neuroimmune; Stem cells; Tissue-specific stem cells
references: Stem Cells. 2006 May;24(5):1294-301
references: 16410387
references: Ann Thorac Surg. 2003 Mar;75(3):775-9
references: 12645692
references: Int J Obes Relat Metab Disord. 2000 Nov;24 Suppl 4:S41-4
references: 11126240
references: Clin Dev Immunol. 2012;2012:970789
references: 22203863
references: Arch Mal Coeur Vaiss. 2005 Sep;98(9):922-6
references: 16231580
references: Tissue Eng Part A. 2009 Jul;15(7):1579-89
references: 19061425
references: J Tissue Eng Regen Med. 2013 Nov;7(11):921-4
references: 22610974
references: Neurosci Lett. 2008 Dec 19;448(1):71-3
references: 18950680
references: Ann Neurol. 2007 Mar;61(3):219-27
references: 17387730
references: Stain Technol. 1981 May;56(3):149-54
references: 6168034
references: Tissue Eng Part C Methods. 2008 Sep;14(3):233-42
references: 18781836
references: Circulation. 2004 Feb 10;109(5):656-63
references: 14734516
references: Transpl Immunol. 2011 Jul;25(1):7-15
references: 21635949
references: Curr Protoc Neurosci. 2001 May;Chapter 9:Unit9.7
references: 18428555
references: Blood. 2005 Sep 1;106(5):1755-61
references: 15905186
references: Tissue Eng. 2001 Apr;7(2):211-28
references: 11304456
references: Acta Med Scand. 1984;215(5):447-51
references: 6741607
references: Biol Blood Marrow Transplant. 2004 Jun;10(6):405-14
references: 15148494
references: Stem Cells. 2007 Apr;25(4):818-27
references: 17420225
references: Proteomics. 2012 Feb;12(4-5):607-20
references: 22246603
references: Nat Rev Endocrinol. 2010 Apr;6(4):195-213
references: 20195269
references: J Cell Physiol. 2007 Sep;212(3):702-9
references: 17477371
references: Arthritis Rheum. 2005 Aug;52(8):2521-9
references: 16052568
references: Proc Natl Acad Sci U S A. 2012 Aug 7;109(32):13064-9
references: 22826216
references: Mol Biol Cell. 2002 Dec;13(12):4279-95
references: 12475952
references: Stem Cells Dev. 2010 May;19(5):657-68
references: 19886804
references: J Immunol. 2009 May 15;182(10):5994-6002
references: 19414750
references: J Manag Care Pharm. 2010 Apr;16(3):206-16
references: 20331325
references: FEBS Lett. 2005 Jul 4;579(17):3487-92
references: 15953605
references: Cells Tissues Organs. 2003;174(3):101-9
references: 12835573
references: Stem Cells Dev. 2011 Apr;20(4):661-70
references: 20698764
references: Biochem Biophys Res Commun. 2010 Oct 1;400(4):461-5
references: 20708604
references: Arch Neurol. 2008 Jun;65(6):753-61
references: 18541795
references: Stem Cells. 2009 Oct;27(10):2624-35
references: 19676124
references: Immunol Lett. 2011 Mar 30;135(1-2):10-6
references: 20888363
references: J Cell Physiol. 2000 Oct;185(1):98-106
references: 10942523
references: J Cell Biochem. 2006 Dec 1;99(5):1285-97
references: 16795045
references: J Mol Neurosci. 2010 May;41(1):129-37
references: 19902385
references: Glia. 2009 Aug 15;57(11):1192-203
references: 19191336
references: Stem Cell Res Ther. 2010 Jun 29;1(2):19
references: 20587076
references: J Transl Med. 2009 Apr 24;7:29
references: 19393041
references: Iran J Allergy Asthma Immunol. 2005 Sep;4(3):113-7
references: 17301432
references: Curr Opin Mol Ther. 2005 Aug;7(4):300-5
references: 16121695
references: J Clin Invest. 2003 Dec;112(12):1796-808
references: 14679176
references: Diabetes Metab. 2006 Nov;32(5 Pt 1):393-401
references: 17110894