title: Signal amplification strategy for sensitive immunoassay of prostate specific antigen (PSA) based on ferrocene incorporated polystyrene spheres.
abstract: A new kind of signal amplification strategy based on ferrocene (Fc) incorporated polystyrene spheres (PS-Fc) was proposed. The synthesized PS-Fc displayed narrow size distribution and good stability. PS-Fc was applied as label to develop immunosensors for prostate specific antigen (PSA) after the typical sandwich immunoreaction by linking anti-PSA antibody (Ab2) onto PS-Fc. After the fabrication of the immunosensor, tetrahydrofuran (THF) was dropped to dissolve PS and release the contained Fc for the following stripping voltammetric detection. PS-Fc as a new electrochemical label prevented the leakage of Fc and greatly amplified the immunosensor signal. In addition, the good biocompatibility of PS could maintain the bioactivity of the antibodies. The response current was linear to the logarithm of PSA concentration in the range from 0.01 ng mL(-1) to 20 ng mL(-1) with a detection limit of 1 pg mL(-1). The immunosensor results were validated through the detection of PSA in serum samples with satisfactory results.
authors: Shandong Provincial Key Laboratory of Life-organic Analysis, College of Chemistry and Chemical Engineering, Qufu Normal University, Qufu Shandong 273165, China.
authors: Lu
authors: L
authors: Ding
authors: Jinmao
authors: J
authors: You
authors: Rongmei
authors: R
authors: Kong
authors: Fengli
authors: F
authors: Qu
mesh_terms: D000906:Antibodies / Q000276:immunology; D055664:Electrochemical Techniques; D005296:Ferrous Compounds / Q000737:chemistry* / Q000378:metabolism; D006108:Graphite / Q000737:chemistry; D006801:Humans; D007118:Immunoassay; D008297:Male; D000075163:Metallocenes; D010087:Oxides / Q000737:chemistry; D011137:Polystyrenes / Q000737:chemistry*; D017430:Prostate-Specific Antigen / Q000097:blood* / Q000276:immunology
chemical_list: D000906:Antibodies; D005296:Ferrous Compounds; D000075163:Metallocenes; D010087:Oxides; D011137:Polystyrenes; D006108:Graphite; D017430:Prostate-Specific Antigen; C004998:ferrocene
keywords: Ferrocene; Graphene oxide; Immunosensor; Polystyrene sphere; Prostate specific antigen