title: ε subunit of Bacillus subtilis F1-ATPase relieves MgADP inhibition.
abstract: MgADP inhibition, which is considered as a part of the regulatory system of ATP synthase, is a well-known process common to all F1-ATPases, a soluble component of ATP synthase. The entrapment of inhibitory MgADP at catalytic sites terminates catalysis. Regulation by the ε subunit is a common mechanism among F1-ATPases from bacteria and plants. The relationship between these two forms of regulatory mechanisms is obscure because it is difficult to distinguish which is active at a particular moment. Here, using F1-ATPase from Bacillus subtilis (BF1), which is strongly affected by MgADP inhibition, we can distinguish MgADP inhibition from regulation by the ε subunit. The ε subunit did not inhibit but activated BF1. We conclude that the ε subunit relieves BF1 from MgADP inhibition.
authors: Department of Life Science, Rikkyo University, Tokyo, Japan.
authors: Junya
authors: J
authors: Mizumoto
authors: Yuka
authors: Y
authors: Kikuchi
authors: Yo-Hei
authors: YH
authors: Nakanishi
authors: Naoto
authors: N
authors: Mouri
authors: Anrong
authors: A
authors: Cai
authors: Tokushiro
authors: T
authors: Ohta
authors: Takamitsu
authors: T
authors: Haruyama
authors: Yasuyuki
authors: Y
authors: Kato-Yamada
mesh_terms: D000244:Adenosine Diphosphate / Q000378:metabolism*; D000255:Adenosine Triphosphate / Q000378:metabolism; D001412:Bacillus subtilis / Q000378:metabolism*; D002384:Catalysis; D004789:Enzyme Activation; D006868:Hydrolysis; D007700:Kinetics; D009154:Mutation; D021122:Protein Subunits / Q000235:genetics / Q000378:metabolism*; D006180:Proton-Translocating ATPases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
chemical_list: D021122:Protein Subunits; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D006180:Proton-Translocating ATPases
references: Nucleic Acids Res. 1988 Aug 11;16(15):7351-67
references: 3045756
references: Proc Natl Acad Sci U S A. 2001 Jun 5;98(12):6560-4
references: 11381110
references: Biochim Biophys Acta. 2002 Feb 15;1553(3):188-211
references: 11997128
references: Biochim Biophys Acta. 1995 Sep 12;1231(2):139-46
references: 7662694
references: J Biol Chem. 2011 Apr 15;286(15):13423-9
references: 21345803
references: EMBO J. 2006 Oct 4;25(19):4596-604
references: 16977308
references: Nat Struct Mol Biol. 2011 Jun;18(6):701-7
references: 21602818
references: Biochem J. 2011 Jul 1;437(1):135-40
references: 21510843
references: Annu Rev Biochem. 1997;66:717-49
references: 9242922
references: Nature. 1970 Aug 15;227(5259):680-5
references: 5432063
references: Proc Natl Acad Sci U S A. 2001 Nov 20;98(24):13649-54
references: 11707579
references: Biophysics (Nagoya-shi). 2010 Dec 17;6:59-65
references: 27857586
references: Essays Biochem. 2000;35:3-18
references: 12471886
references: J Biol Chem. 2007 Dec 28;282(52):37618-23
references: 17933866
references: J Bacteriol. 1994 Nov;176(22):6802-11
references: 7961438
references: Anal Biochem. 1976 May 7;72:248-54
references: 942051
references: J Biol Chem. 2003 Sep 19;278(38):36013-6
references: 12837747
references: Methods Enzymol. 1979;55:308-15, 819-21
references: 156835
references: Nat Rev Mol Cell Biol. 2001 Sep;2(9):669-77
references: 11533724
references: Biochem J. 1982 Jan 15;202(1):15-23
references: 6211171
references: FEBS Lett. 2009 Apr 2;583(7):1121-6
references: 19265694
references: Biochim Biophys Acta. 1995 Oct 10;1231(3):275-81
references: 7578215
references: Gene. 1989 Apr 15;77(1):51-9
references: 2744487
references: J Bacteriol. 1987 Oct;169(10):4743-9
references: 2888751
references: J Bacteriol. 1994 Jul;176(13):4192-5
references: 8021203
references: Arch Biochem Biophys. 1979 Oct 1;197(1):83-9
references: 161698
references: Biochemistry. 1995 Dec 19;34(50):16412-8
references: 8845368
references: J Biol Chem. 2010 Dec 31;285(53):42058-67
references: 20974856
references: J Biol Chem. 1997 Mar 28;272(13):8215-21
references: 9079639
references: Biochemistry. 1988 Jul 12;27(14):5129-35
references: 2901855
references: Biochem Biophys Res Commun. 2009 Dec 11;390(2):230-4
references: 19785990
references: J Biol Chem. 2005 Dec 2;280(48):40130-4
references: 16203732
references: J Supramol Struct. 1975;3(3):248-55
references: 127087
references: Cell. 1998 Jun 26;93(7):1117-24
references: 9657145
references: J Biol Chem. 1992 Mar 5;267(7):4551-6
references: 1531655
references: J Biol Chem. 1996 Nov 15;271(46):28818-24
references: 8910526
references: Nat Struct Biol. 2000 Nov;7(11):1051-4
references: 11062562
references: J Biol Chem. 1997 Oct 3;272(40):24906-12
references: 9312092
references: Biochem J. 1982 Jan 15;202(1):9-14
references: 6211173
references: Biophys J. 2010 Feb 3;98(3):434-42
references: 20141757
references: Nature. 1997 Mar 20;386(6622):299-302
references: 9069291
references: J Biol Chem. 2007 Jan 5;282(1):764-72
references: 17092944
references: J Biol Chem. 1998 Jan 9;273(2):865-70
references: 9422743
references: Eur J Biochem. 1999 Jun;262(2):563-8
references: 10336643
references: J Biol Chem. 1993 Nov 5;268(31):23179-85
references: 8226836
references: J Biol Chem. 1994 Nov 18;269(46):28871-7
references: 7961847
references: J Biol Chem. 1991 Jun 25;266(18):11551-8
references: 1828802
references: J Biol Chem. 2003 Nov 21;278(47):46840-6
references: 12881515