title: Detection and quantification of chimerism by droplet digital PCR.
abstract: Accurate quantification of chimerism and microchimerism is proving to be increasingly valuable for hematopoietic cell transplantation as well as non-transplant conditions. However, methods that are available to quantify low-level chimerism lack accuracy. Therefore, we developed and validated a method for quantifying chimerism based on digital PCR technology. We demonstrate accurate quantification that far exceeds what is possible with analog qPCR down to 0.01% with the potential to go even lower. Also, this method is inherently more informative than qPCR. We expect the advantages of digital PCR will make it the preferred method for chimerism analysis.
authors: Department of Pathology and Laboratory Medicine; Ann & Robert H. Lurie Children's Hospital of Chicago; Chicago, IL USA.
authors: David
authors: D
authors: George
authors: Juliann
authors: J
authors: Czech
authors: Bobby
authors: B
authors: John
authors: Min
authors: M
authors: Yu
authors: Lawrence J
authors: LJ
authors: Jennings
mesh_terms: D053523:Amelogenin / Q000235:genetics; D046528:Chimerism; D041321:Chromosomes, Human, X; D041322:Chromosomes, Human, Y; D004247:DNA / Q000032:analysis*; D003627:Data Interpretation, Statistical; D005260:Female; D014644:Genetic Variation; D015894:Genome, Human; D006801:Humans; D008297:Male; D009154:Mutation; D016133:Polymerase Chain Reaction / Q000379:methods*; D033581:Stem Cell Transplantation; D018183:Transplantation Chimera
chemical_list: D053523:Amelogenin; D004247:DNA
keywords: PCR; chimerism; digital; informative loci; limit of detection; microchimerism; quantitative
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