title: Improvement of strain Penicillium sp. EZ-ZH190 for tannase production by induced mutation.
abstract: In the search for an efficient producer of tannase, Penicillium sp. EZ-ZH190 was subjected to mutagenesis using heat treatment and strain EZ-ZH290 was isolated. The maximum tannase in this mutant strain was 4.32 U/mL with an incubation period of 84 h as compared to wild strain EZ-ZH190 where the incubation period was 96 h with a maximum enzyme activity of 4.33 U/mL. Also, the Penicillium sp. EZ-ZH290 tannase had a maximum activity at 40 °C and pH 5.5. Then, the spores of strain EZ-ZH290 were subjected to γ irradiation mutagenesis and strain EZ-ZH390 was isolated. Strain EZ-ZH390 exhibited higher tannase activity (7.66 U/mL) than the parent strain EZ-ZH290. It was also found that Penicillium sp. EZ-ZH390 tannase had an optimum activity at 35 °C and a broad pH profile with an optimum at pH 5.5. The tannase pH stability of Penicillium sp. EZ-ZH390 and its maximum production of tannase followed the same trend for five generations confirming the occurrence of stable mutant. This paper is shown that γ irradiation can mutate the Penicillium sp. leading to increase the tannase production.
authors: Food Science and Technology Department, Faculty of Agriculture, Tarbiat Modares University, Ale-Ahmad St., Tehran, Iran.
authors: E
authors: E
authors: Zakipour-Molkabadi
authors: Z
authors: Z
authors: Hamidi-Esfahani
authors: M A
authors: MA
authors: Sahari
authors: M H
authors: MH
authors: Azizi
mesh_terms: D002265:Carboxylic Ester Hydrolases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D004795:Enzyme Stability; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D016296:Mutagenesis; D009154:Mutation; D010407:Penicillium / Q000737:chemistry / Q000201:enzymology* / Q000235:genetics*; D013696:Temperature
chemical_list: D005656:Fungal Proteins; D002265:Carboxylic Ester Hydrolases; C022711:tannase