title: Bradykinin preconditioning affects the number of degenerated neurons and the level of antioxidant enzymes in spinal cord ischemia in rabbits.
abstract: Bradykinin preconditioning has been used for acquisition of tolerance after spinal cord ischemia. Rabbits were preconditioned intraperitoneally with bradykinin 48 h prior to 20 min of abdominal aorta ligation followed by 24 and 48 h of reperfusion. The activities of SOD and catalase were measured and Fluoro Jade B (FJB)-positive degenerated neurons were evaluated. The outcomes of Tarlov scoring system used to assess neurological functions showed significant improvement in bradykinin groups compared to the ischemic group. The number of FJB-positive degenerated neurons was decreased in ventral horns of both bradykinin groups. Significantly decreased activities of total SOD and mitochondrial Mn-SOD were also detected in both bradykinin groups versus ischemic group while CuZn-SOD and catalase activities were significantly decreased only in the bradykinin group after 24h of reperfusion versus ischemic group. These findings suggest that one of the possibilities of the neuroprotective effect of delayed bradykinin preconditioning against spinal cord ischemic injury could be realized by mitochondrial protection and decreased synthesis of Mn-SOD as well as by promotion of neuronal survival.
authors: Department of Histology and Embryology, Faculty of Medicine, P. J. Šafárik University, Šrobárova 2, 041 80 Košice, Slovak Republic. Electronic address: eva.mechir@gmail.com.
authors: Eva
authors: E
authors: Mechírová
authors: Institute of Neurobiology, Slovak Academy of Sciences, Šoltésovej 4, 040 01 Košice, Slovak Republic.
authors: Viera
authors: V
authors: Danielisová
authors: Department of Histology and Embryology, Faculty of Medicine, P. J. Šafárik University, Šrobárova 2, 041 80 Košice, Slovak Republic.
authors: Iveta
authors: I
authors: Domoráková
authors: Department of Histology and Embryology, Faculty of Medicine, P. J. Šafárik University, Šrobárova 2, 041 80 Košice, Slovak Republic.
authors: Marianna
authors: M
authors: Danková
authors: 2nd Department of Surgery, Faculty of Medicine, P. J. Šafárik University, Rastislavova 43, 041 90 Košice, Slovak Republic.
authors: Milan
authors: M
authors: Stebnický
authors: Department of Medical Biology, Faculty of Medicine, P. J. Šafárik University, Tr. SNP 1, 040 11 Košice, Slovak Republic.
authors: Helena
authors: H
authors: Mičková
authors: Institute of Neurobiology, Slovak Academy of Sciences, Šoltésovej 4, 040 01 Košice, Slovak Republic.
authors: Jozef
authors: J
authors: Burda
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D001920:Bradykinin / Q000494:pharmacology*; D002374:Catalase / Q000378:metabolism; D007511:Ischemia / Q000188:drug therapy / Q000201:enzymology / Q000473:pathology*; D019194:Ischemic Preconditioning; D008297:Male; D009410:Nerve Degeneration / Q000201:enzymology / Q000517:prevention & control; D009474:Neurons / Q000187:drug effects / Q000378:metabolism; D018696:Neuroprotective Agents / Q000494:pharmacology*; D011817:Rabbits; D013116:Spinal Cord / Q000098:blood supply* / Q000187:drug effects / Q000201:enzymology; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D000975:Antioxidants; D018696:Neuroprotective Agents; D002374:Catalase; D013482:Superoxide Dismutase; D001920:Bradykinin
keywords: Bradykinin preconditioning; Fluoro Jade B; Rabbit; SOD; Spinal cord ischemia