title: A Tet-on system for DRD1-expressing cells.
abstract: Cells expressing the dopamine D1 receptor (DRD1) have significant functional roles in diverse physiological processes including locomotion and drug addiction. The present work presents a novel in vivo DRD1-Bacterial Artificial Chromosome (BAC) Tet-on system allowing for the inducible activation of tet-operated transgenes specifically within DRD1-expressing cells of transgenic mice. It is shown that the DRD1-rtTA BAC-driven expression of a tet-operated reporter is under tight regulation by doxycycline and is restricted to DRD1-expressing brain regions. The model will be a useful research tool in studies of movement and reward and associated pathologies such as Parkinson's disease and addiction.
authors: Department of Psychiatry and Behavioral Neurosciences, Wayne State University School of Medicine, Detroit, Michigan, USA.
authors: Fayi
authors: F
authors: Yao
authors: Paul D
authors: PD
authors: Walker
authors: Robert G
authors: RG
authors: MacKenzie
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism; D002460:Cell Line; D022202:Chromosomes, Artificial, Bacterial; D005786:Gene Expression Regulation / Q000187:drug effects*; D023061:Gene Order; D017930:Genes, Reporter; D005822:Genetic Vectors; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D011401:Promoter Regions, Genetic; D017447:Receptors, Dopamine D1 / Q000235:genetics* / Q000378:metabolism; D013752:Tetracycline / Q000494:pharmacology*; D015533:Transcriptional Activation / Q000187:drug effects
chemical_list: D017447:Receptors, Dopamine D1; D013752:Tetracycline
references: Nature. 1979 Jan 11;277(5692):93-6
references: 215920
references: Annu Rev Neurosci. 2011;34:441-66
references: 21469956
references: Neuron. 2012 Jun 7;74(5):858-73
references: 22681690
references: Pharmacol Rev. 2011 Mar;63(1):182-217
references: 21303898
references: Neuron. 2012 Jan 12;73(1):108-20
references: 22243750
references: J Neurosci. 2008 May 21;28(21):5504-12
references: 18495884
references: PLoS One. 2007 Jun 20;2(6):e533
references: 17579707
references: J Neurosci. 2011 Jan 26;31(4):1183-92
references: 21273403
references: Trends Neurosci. 2009 Oct;32(10):538-47
references: 19765834
references: J Neurosci. 2007 Sep 12;27(37):9817-23
references: 17855595
references: Science. 1996 Dec 6;274(5293):1678-83
references: 8939850
references: J Neurosci. 2010 Apr 21;30(16):5713-23
references: 20410123
references: J Neurosci. 2008 Mar 12;28(11):2681-5
references: 18337395
references: Genesis. 2008 Jul;46(7):357-67
references: 18615733
references: Methods Enzymol. 2000;327:401-21
references: 11044999
references: Nat Rev Genet. 2001 Oct;2(10):769-79
references: 11584293
references: Front Syst Neurosci. 2011 Jun 08;5:32
references: 21713123
references: Annu Rev Neurosci. 2009;32:127-47
references: 19400717
references: Methods Mol Biol. 2009;530:325-42
references: 19266340
references: FASEB J. 2010 Jul;24(7):2427-35
references: 20223941
references: Neuron. 2010 Jun 24;66(6):896-907
references: 20620875
references: Genome Res. 2003 Mar;13(3):476-84
references: 12618378
references: Front Neuroanat. 2011 Aug 11;5:51
references: 21886608
references: J Neurosci. 2005 Jul 13;25(28):6651-7
references: 16014726
references: Trends Neurosci. 1992 Apr;15(4):133-9
references: 1374971
references: Nature. 2003 Oct 30;425(6961):917-25
references: 14586460
references: Genes Dev. 2001 Mar 15;15(6):652-7
references: 11274051
references: Front Behav Neurosci. 2011 Oct 24;5:71
references: 22028687