title: A semi-automated method for isolating functionally intact mitochondria from cultured cells and tissue biopsies.
abstract: Mitochondrial dysfunctions decisively contribute to the progression of human diseases, implying that functional tests of isolated mitochondria may furnish conclusive information for diagnosis and therapy. Classical mitochondrial isolation methods, however, lack precisely adjustable settings for cell rupture, which is the most critical step in this procedure, and this complicates subsequent analyses. Here, we present an efficient method to isolate functionally active, intact mitochondria from cultured or primary cells and minute tissue samples in a rapid, highly reproducible manner.
authors: Institute of Molecular Toxicology and Pharmacology, Helmholtz Center Munich, German Research Center for Environmental Health, D-85764 Neuherberg, Germany.
authors: Sabine
authors: S
authors: Schmitt
authors: Friederike
authors: F
authors: Saathoff
authors: Lilja
authors: L
authors: Meissner
authors: Eva-Maria
authors: EM
authors: Schropp
authors: Josef
authors: J
authors: Lichtmannegger
authors: Sabine
authors: S
authors: Schulz
authors: Carola
authors: C
authors: Eberhagen
authors: Sabine
authors: S
authors: Borchard
authors: Michaela
authors: M
authors: Aichler
authors: Jerzy
authors: J
authors: Adamski
authors: Nikolaus
authors: N
authors: Plesnila
authors: Simon
authors: S
authors: Rothenfusser
authors: Guido
authors: G
authors: Kroemer
authors: Hans
authors: H
authors: Zischka
mesh_terms: D000818:Animals; D057205:Automation, Laboratory; D015415:Biomarkers / Q000378:metabolism; D002458:Cell Fractionation; D045744:Cell Line, Tumor; D022781:Hepatocytes / Q000378:metabolism / Q000648:ultrastructure*; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL; D008930:Mitochondria, Liver / Q000378:metabolism / Q000648:ultrastructure*; D009474:Neurons / Q000378:metabolism / Q000648:ultrastructure*; D061251:Primary Cell Culture; D051381:Rats; D011915:Rats, Inbred BUF; D011921:Rats, Inbred WKY
chemical_list: D015415:Biomarkers
keywords: Balch homogenizer; Biopsies; Cell culture; Mitochondria