title: Preliminary molecular analysis of bacterial composition in periapical lesions with primary endodontic infections of deciduous teeth.
abstract: BACKGROUND
  The bacterial composition of periapical lesions in deciduous teeth has not been well documented. This study was designed to explore the bacterial compositions, especially the dominant bacteria in periapical lesions using 16S rRNA sequencing.
  
  
  METHODS
  Tissue samples were collected from 11 periapical lesions in deciduous teeth with primary endodontic infections. DNA was extracted from each sample and analyzed using 16S rRNA cloning and sequencing for the identification of bacteria.
  
  
  RESULTS
  All DNA samples were positive for 16S rRNA gene PCR. One hundred and fifty-one phylotypes from 810 clones were identified to eight phyla, and each sample contained an average of 25.9 phylotypes. In addition, 59 phylotypes were detected in more than two samples, and Fusobacterium (F.) nucleatum (8/11), Dialister (D.) invisus (8/11), Campylobacter (C.) gracilis (7/11), Escherichia (E.) coli DH1 (6/11), Aggregatibacter (A.) segnis (6/11), and Streptococcus (S.) mitis (6/11) were the most prevalent species. Furthermore, 45 as-yet-uncultivated phylotypes were also identified.
  
  
  CONCLUSIONS
  Chronic periapical lesions in deciduous teeth contained polymicrobial infections. F. nucleatum, D. invisus, C. gracilis, E. coli DH1, A. segnis, and S. mitis were the most prevalent species detected by 16S rRNA sequencing.
authors: Capital Medical University School of Stomatology, Beijing, China.
authors: Jia-jian
authors: JJ
authors: Shang
authors: Qiu-bo
authors: QB
authors: Yang
authors: Huan-ying
authors: HY
authors: Zhao
authors: Shuang
authors: S
authors: Cai
authors: Yan
authors: Y
authors: Zhou
authors: Zheng
authors: Z
authors: Sun
mesh_terms: D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D001424:Bacterial Infections / Q000382:microbiology*; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D008297:Male; D010486:Periapical Tissue / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D014094:Tooth, Deciduous / Q000382:microbiology*
chemical_list: D012336:RNA, Ribosomal, 16S