title: Nanoparticle for delivery of antisense γPNA oligomers targeting CCR5.
abstract: The development of a new class of peptide nucleic acids (PNAs), i.e., gamma PNAs (γPNAs), creates the need for a general and effective method for its delivery into cells for regulating gene expression in mammalian cells. Here we report the antisense activity of a recently developed hydrophilic and biocompatible diethylene glycol (miniPEG)-based gamma peptide nucleic acid called MPγPNAs via its delivery by poly(lactide-co-glycolide) (PLGA)-based nanoparticle system. We show that MPγPNA oligomers designed to bind to the selective region of chemokine receptor 5 (CC R5) transcript, induce potent and sequence-specific antisense effects as compared with regular PNA oligomers. In addition, PLGA nanoparticle delivery of MPγPNAs is not toxic to the cells. The findings reported in this study provide a combination of γPNA technology and PLGA-based nanoparticle delivery method for regulating gene expression in live cells via the antisense mechanism.
authors: Raman
authors: R
authors: Bahal
authors: Nicole Ali
authors: NA
authors: McNeer
authors: Danith H
authors: DH
authors: Ly
authors: W Mark
authors: WM
authors: Saltzman
authors: Peter M
authors: PM
authors: Glazer
mesh_terms: D001483:Base Sequence; D015870:Gene Expression; D055785:Gene Knockdown Techniques; D020868:Gene Silencing; D006367:HeLa Cells; D006801:Humans; D007700:Kinetics; D053769:Nanocapsules / Q000737:chemistry*; D016376:Oligonucleotides, Antisense / Q000737:chemistry / Q000235:genetics; D010316:Particle Size; D020135:Peptide Nucleic Acids / Q000737:chemistry / Q000235:genetics*; D019713:Receptors, CCR5 / Q000235:genetics / Q000378:metabolism; D014162:Transfection / Q000379:methods
chemical_list: C584933:CCR5 protein, human; D053769:Nanocapsules; D016376:Oligonucleotides, Antisense; D020135:Peptide Nucleic Acids; D019713:Receptors, CCR5
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