title: An insight into the lignin peroxidase of Macrophomina phaseolina.
abstract: Macrophomina phaseolina is one of the deadliest necrotrophic fungal pathogens that infect more than 500 plant species including major food, fiber, and oil crops all throughout the globe. It secretes a cocktail of ligninolytic enzymes along with other hydrolytic enzymes for degrading the woody lignocellulosic plant cell wall and penetrating into the host tissue. Among them, lignin peroxidase has been reported only in Phanerochaete chrysosporium so far. But interestingly, a recent study has revealed a second occurrence of lignin peroxidase in M. phaseolina. However, lignin peroxidases are of much significance biotechnologically because of their potential applications in bio-remedial waste treatment and in catalyzing difficult chemical transformations. Besides, this enzyme also possesses agricultural and environmental importance on account of their role in lignin biodegradation. In the present work, different properties of the lignin peroxidase of M. phaseolina along with predicting the 3-D structure and its active sites were investigated by the use of various computational tools. The data from this study will pave the way for more detailed exploration of this enzyme in wet lab and thereby facilitating the strategies to be designed against such deadly weapons of Macrophomina phaseolina. Furthermore, the insight of such a ligninolytic enzyme will contribute to the assessment of its potentiality as a bioremediation tool.
authors: Department of Genetic Engineering and Biotechnology, Faculty of Biological Sciences, University of Chittagong, Chittagong - 4331, Bangladesh.
authors: Mohammed Touaha
authors: MT
authors: Akbar
authors: Abdul Musaweer
authors: AM
authors: Habib
authors: Dil Umme Salma
authors: DU
authors: Chowdhury
authors: Md Iqbal Kaiser
authors: MI
authors: Bhuiyan
authors: Kazi Md Golam
authors: KM
authors: Mostafa
authors: Sobuj
authors: S
authors: Mondol
authors: Ivan Mhai
authors: IM
authors: Mosleh
keywords: Ligin peroxidase; Macrophomina phaseolina; lignin
references: Bioinformation. 2012;8(18):891-6
references: 23144546
references: Curr Protein Pept Sci. 2006 Oct;7(5):395-406
references: 17073692
references: Nat Protoc. 2009;4(3):363-71
references: 19247286
references: Biochem J. 1996 Apr 1;315 ( Pt 1):15-9
references: 8670100
references: Appl Environ Microbiol. 1995 Mar;61(3):1098-103
references: 16534959
references: Nat Rev Genet. 2001 Jul;2(7):493-503
references: 11433356
references: Proc Natl Acad Sci U S A. 1984 Apr;81(8):2280-4
references: 16593451
references: Microbiol Rev. 1993 Sep;57(3):605-22
references: 8246842
references: BMC Genomics. 2012 Sep 19;13:493
references: 22992219
references: BMC Struct Biol. 2006 Sep 24;6:19
references: 16995956
references: Appl Environ Microbiol. 1993 Sep;59(9):2897-903
references: 8215362
references: Nucleic Acids Res. 2003 Jul 1;31(13):3381-5
references: 12824332
references: Proteins. 1992 Apr;12(4):345-64
references: 1579569
references: Nucleic Acids Res. 2006 Jul 1;34(Web Server issue):W116-8
references: 16844972
references: BMC Bioinformatics. 2011 Mar 31;12:90
references: 21453511