title: A luminol chemiluminescence method for sensing histidine and lysine using enzyme reactions.
abstract: The analysis of free amino acids in urine and plasma is useful for estimating disease status in clinical diagnoses. Changes in the concentration of free amino acids in foods are also useful markers of freshness, nutrition, and taste. In this study, the specific interaction between aminoacyl-tRNA synthetase (aaRS) and its corresponding amino acid was used to measure amino acid concentrations. Pyrophosphate released by the amino acid-aaRS binding reaction was detected by luminol chemiluminescence; the method provided selective quantitation of 1.0-30 μM histidine and 1.0-60 μM lysine.
authors: Center for Industry and Public Relations, Hiroshima City University, Naka-ku, Hiroshima 730-0052, Japan. Electronic address: kugimiya@hiroshima-cu.ac.jp.
authors: Akimitsu
authors: A
authors: Kugimiya
authors: Rie
authors: R
authors: Fukada
authors: Daiki
authors: D
authors: Funamoto
mesh_terms: D000604:Amino Acyl-tRNA Synthetases / Q000737:chemistry*; D002138:Calibration; D011756:Diphosphates / Q000737:chemistry; D057075:Enzyme Assays; D006639:Histidine / Q000032:analysis*; D006861:Hydrogen Peroxide / Q000737:chemistry; D043564:Inorganic Pyrophosphatase / Q000737:chemistry; D057230:Limit of Detection; D049449:Luminescence; D008163:Luminescent Measurements; D008165:Luminol / Q000737:chemistry*; D008239:Lysine / Q000032:analysis*; D011485:Protein Binding; D011771:Pyruvate Oxidase / Q000737:chemistry; D015203:Reproducibility of Results; D012996:Solutions; D013696:Temperature
chemical_list: D011756:Diphosphates; D012996:Solutions; C107241:diphosphoric acid; D006639:Histidine; D008165:Luminol; D006861:Hydrogen Peroxide; D011771:Pyruvate Oxidase; D043564:Inorganic Pyrophosphatase; D000604:Amino Acyl-tRNA Synthetases; D008239:Lysine
keywords: Amino acid; Aminoacyl–tRNA synthetase; Biosensing; Enzyme reaction; Luminol chemiluminescence