title: [The association of killer cell immunoglobulin like receptor gene polymorphism with cytomegalovirus infection after hematopoietic stem cell transplantation].
abstract: OBJECTIVE
  To explore the influence of the killer cell immunoglobulin like receptor (KIR) gene polymorphism on cytomegalovirus (CMV) infection and pathogenesis after hematopoietic stem cell transplantation (HSCT).
  
  
  METHODS
  The KIR genotype was determined by sequence-specific primer polymerase chain reaction (PCR-SSP) in 138 pairs of donors and recipients before HSCT during October, 2005 and May, 2011. Posttransplant monitoring for CMVpp65 antigen was performed by indirect immune histochemically assays since week 2 after transplantation. The differences between CMV positive group and negative group, inhibitive and active KIR of donors and recipients, and KIR haplotype frequency of donors and recipients were analyzed.
  
  
  RESULTS
  There were no significant differences in frequency of KIR gene and haplotype AA, AB, BB between the donors and recipients. The frequencies of 2DS2 and 2DS4 * 003-007 of donors in CMV positive group were obviously lower than those in CMV negative group with significant differences (8% vs 16% , P = 0.0420; 3% vs 13%, P = 0.0050). There was no significant difference in KIR gene between CMV positive group and CMV negative group. The CMV infection rates of haplotype AA, BB, AB donors were 64.38%, 36.84% and 50.00%, while CMV infection rates of haplotype AA, BB, AB recipients were 53.73%, 46.15% and 51.72%, respectively. The CMV infection rate was higher in the patients received KIR haplotype AA donor than in those received KIR haplotype BB donor (36.84% vs 64.38%, P = 0.0299). 2DS4 x 003-007 and haplotype BB of donor were found associated with CMV infection in multifactor analysis.
  
  
  CONCLUSIONS
  KIR genotypes of donors are associated with CMV infection after HSCT.
authors: Jiangsu Institute of Hematology, Key Laboratory of Thrombosis and Hemostasis of Ministry of Health, the First Affiliated Hospital of Soochow University, Suzhou 215006, China.
authors: Xiao-Jin
authors: XJ
authors: Wu
authors: Jun
authors: J
authors: He
authors: De-Pei
authors: DP
authors: Wu
authors: Xiao-Jing
authors: XJ
authors: Bao
authors: Chao
authors: C
authors: Xu
authors: Hui-Fen
authors: HF
authors: Zhou
authors: Ai-Ning
authors: AN
authors: Sun
authors: Yue
authors: Y
authors: Han
authors: Xiao-Wen
authors: XW
authors: Tang
authors: Zheng-Zheng
authors: ZZ
authors: Fu
authors: Xiao
authors: X
authors: Ma
mesh_terms: D000293:Adolescent; D000328:Adult; D002648:Child; D003586:Cytomegalovirus Infections / Q000235:genetics*; D005260:Female; D018380:Hematopoietic Stem Cell Transplantation; D006801:Humans; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic; D054340:Receptors, KIR / Q000235:genetics*; D055815:Young Adult
chemical_list: D054340:Receptors, KIR