title: Genistein affects parathyroid gland and NaPi 2a cotransporter in an animal model of the andropause.
abstract: This study aimed to examine the effects of genistein on the structural and functional changes in parathyroid glands (PTG) and sodium phosphate cotransporter 2a (NaPi 2a) in orchidectomized rats. Sixteen-month-old Wistar rats were divided into sham-operated (SO), orchidectomized (Orx) and genistein-treated orchidectomized (Orx+G) groups. Genistein (30 mg/kg/day) was administered subcutaneously for 3 weeks, while the controls received vehicle alone. PTG was analyzed histomorphometrically, while the expressions of NaPi 2a mRNA/protein levels from kidneys were determined by real time PCR and Western blots. Serum and urine parameters were determined biochemically. The PTG volume in Orx rats was increased by 30% (p<0.05), compared to the SO group. Orx+G treatment increased the PTG volume by 35% and 75% (p<0.05) respectively, comparing to Orx and SO animals. Orchidectomy led to increment of serum PTH by 27% (p<0.05) compared to the SO group, Orx+G decreased it by 18% (p<0.05) comparing to Orx animals. NaPi 2a expression in Orx animals was reduced in regards to its abundance in SO animals, although it was increased in Orx+G group compared to the Orx. Phosphorus urine content of Orx animals was raised by 12% (p<0.05) compared to that for the SO group, while Orx+G induced a 17% reduction (p<0.05) in regards to Orx animals. Our study shows that Orx increases PTG volume and serum PTH level, while protein expression of NaPi 2a is reduced. Application of genistein attenuates the orchidectomy-induced changes in serum PTH level, stimulates the expression of NaPi 2a and reduces urinary Pi excretion, implying potential beneficial effects on andropausal symptoms.
authors: Department of Cytology, Institute for Biological Research Sinisa Stankovic, University of Belgrade, Belgrade, Serbia. jasminap@ibiss.bg.ac.rs
authors: J
authors: J
authors: Pantelic
authors: V
authors: V
authors: Ajdzanovic
authors: I
authors: I
authors: Medigovic
authors: M
authors: M
authors: Mojic
authors: S
authors: S
authors: Trifunovic
authors: V
authors: V
authors: Milosevic
authors: B
authors: B
authors: Filipovic
mesh_terms: D047649:Andropause; D000818:Animals; D002118:Calcium / Q000097:blood / Q000652:urine; D005786:Gene Expression Regulation / Q000187:drug effects; D019833:Genistein / Q000008:administration & dosage / Q000627:therapeutic use*; D006996:Hypocalcemia / Q000209:etiology / Q000517:prevention & control; D017674:Hypophosphatemia / Q000209:etiology / Q000517:prevention & control; D007279:Injections, Subcutaneous; D007668:Kidney / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism / Q000648:ultrastructure; D008297:Male; D009919:Orchiectomy / Q000009:adverse effects; D009929:Organ Size / Q000187:drug effects; D010280:Parathyroid Glands / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism / Q000648:ultrastructure; D010281:Parathyroid Hormone / Q000097:blood; D010758:Phosphorus / Q000097:blood / Q000652:urine; D048789:Phytoestrogens / Q000008:administration & dosage / Q000627:therapeutic use*; D051381:Rats; D017208:Rats, Wistar; D050613:Sodium-Phosphate Cotransporter Proteins, Type IIa / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D014883:Water-Electrolyte Imbalance / Q000209:etiology / Q000378:metabolism / Q000503:physiopathology / Q000517:prevention & control*
chemical_list: D010281:Parathyroid Hormone; D048789:Phytoestrogens; C493556:Slc34a1 protein, rat; D050613:Sodium-Phosphate Cotransporter Proteins, Type IIa; D010758:Phosphorus; D019833:Genistein; D002118:Calcium