title: First report on class 1 integrons and Trimethoprim-resistance genes fromdfrAgroup in uropathogenicE. coli(UPEC) from the Aleppo area in Syria.
abstract: Horizontal gene transfer (HGT) introduces advantageous genetic elements into pathogenic bacteria using tools such as class1 integrons. This study aimed at investigating the distribution of these integrons among uropathogenicE. coli(UPEC) isolated from patients in Aleppo, Syria. It also set to uncover the frequencies of the clinically relevant DfrA1 and DfrA17,7, as well as various associations leading to reduced susceptibility. This study involved 75 Trimethoprim-resistantE. coliisolates from in- and outpatients with urinary tract infections (UTIs) from 3 major hospitals in Aleppo. Bacterial identification, resistance and extended-spectrum-β-lactamase (ESBL) production testing were performed according to Clinical Laboratory Standards Institute guidelines. Detection of integrons and DfrA genes was done using PCR and statistical significance was inferred through χ2 (Fisher's) test. Class1 integrons were detected in 54.6% of isolates while DfrA1 and DfrA17,7 were found in 16% and 70.6% of tested samples respectively. Furthermore, only DfrA17,7 were strongly associated with class1 integrons, as were reduced susceptibility to the majority of individual antibiotics, multidrug resistance and ESBL production. This study demonstrated the high prevalence of class1 integrons among UPEC strains in Aleppo, Syria, as well as their significant associations with MDR. This data give information for local healthcare provision using antibiotic chemotherapy.
authors: Department of Biochemistry and Microbiology; Faculty of Pharmacy; University of Aleppo; Aleppo, Syria.
authors: Bodour
authors: B
authors: Al-Assil
authors: Maysa
authors: M
authors: Mahfoud
authors: Abdul Rezzak
authors: AR
authors: Hamzeh
keywords: DfrA1; DfrA17,7; antibiotic resistance; urinary tract infections
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