title: Dietary fish oil and DHA down-regulate antigen-activated CD4+ T-cells while promoting the formation of liquid-ordered mesodomains.
abstract: We have demonstrated previously that n-3 PUFA endogenously produced by fat-1 transgenic mice regulate CD4+ T-cell function by affecting the formation of lipid rafts, liquid-ordered mesodomains in the plasma membrane. In the present study, we tested the effects of dietary sources of n-3 PUFA, i.e. fish oil (FO) or purified DHA, when compared with an n-6 PUFA-enriched maize oil control diet in DO11.10 T-cell receptor transgenic mice. Dietary n-3 PUFA were enriched in CD4+ T-cells, resulting in the increase of the n-3:n-6 ratio. Following antigen-specific CD4+ T-cell activation by B-lymphoma cells pulsed with the ovalbumin 323-339 peptide, the formation of liquid-ordered mesodomains at the immunological synapse relative to the whole CD4+ T-cell, as assessed by Laurdan labelling, was increased (P< 0·05) in the FO-fed group. The FO diet also suppressed (P< 0·05) the co-localisation of PKCθ with ganglioside GM1 (monosialotetrahexosylganglioside), a marker for lipid rafts, which is consistent with previous observations. In contrast, the DHA diet down-regulated (P< 0·05) PKCθ signalling by moderately affecting the membrane liquid order at the immunological synapse, suggesting the potential contribution of the other major n-3 PUFA components of FO, including EPA.
authors: Department of Food Science and Biotechnology, Kyung Hee University, Seocheondong, Giheunggu, Yonginsi, Gyunggido, Republic of Korea.
authors: Wooki
authors: W
authors: Kim
authors: Department of Veterinary Integrative Biosciences, Texas A&M University, College Station, TX 77843-4458, USA.
authors: Rola
authors: R
authors: Barhoumi
authors: Program in Integrative Nutrition and Complex Diseases, 321 Kleberg Center, MS 2253, Texas A&M University, College Station, TX 77843-2253, USA.
authors: David N
authors: DN
authors: McMurray
authors: Department of Veterinary Integrative Biosciences, Texas A&M University, College Station, TX 77843-4458, USA.
authors: Robert S
authors: RS
authors: Chapkin
mesh_terms: D000821:Animal Feed / Q000032:analysis; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000187:drug effects / Q000502:physiology*; D002462:Cell Membrane; D003314:Corn Oil / Q000737:chemistry; D004032:Diet / Q000662:veterinary; D004281:Docosahexaenoic Acids / Q000737:chemistry / Q000494:pharmacology*; D015536:Down-Regulation; D043371:Fatty Acids, Omega-6 / Q000737:chemistry / Q000494:pharmacology; D005260:Female; D005395:Fish Oils / Q000494:pharmacology*; D005677:G(M1) Ganglioside / Q000235:genetics / Q000378:metabolism; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism; D008297:Male; D051379:Mice; D011493:Protein Kinase C / Q000235:genetics / Q000378:metabolism; D000074121:Protein Kinase C-theta
chemical_list: D043371:Fatty Acids, Omega-6; D005395:Fish Oils; D007527:Isoenzymes; D004281:Docosahexaenoic Acids; D005677:G(M1) Ganglioside; D003314:Corn Oil; C489593:Prkcq protein, mouse; D011493:Protein Kinase C; D000074121:Protein Kinase C-theta
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