title: The Drosophila T-box transcription factor Midline functions within the Notch-Delta signaling pathway to specify sensory organ precursor cell fates and regulates cell survival within the eye imaginal disc.
abstract: We report that the T-box transcription factor Midline (Mid), an evolutionary conserved homolog of the vertebrate Tbx20 protein, functions within the Notch-Delta signaling pathway essential for specifying the fates of sensory organ precursor (SOP) cells. These findings complement an established history of research showing that Mid regulates the cell-fate specification of diverse cell types within the developing heart, epidermis and central nervous system. Tbx20 has been detected in unique neuronal and epithelial cells of embryonic eye tissues in both mice and humans. However, the mechanisms by which either Mid or Tbx20 function to regulate cell-fate specification or other critical aspects of eye development including cell survival have not yet been elucidated. We have also gathered preliminary evidence suggesting that Mid may play an indirect, but vital role in selecting SOP cells within the third-instar larval eye disc by regulating the expression of the proneural gene atonal. During subsequent pupal stages, Mid specifies SOP cell fates as a member of the Notch-Delta signaling hierarchy and is essential for maintaining cell viability by inhibiting apoptotic pathways. We present several new hypotheses that seek to understand the role of Mid in regulating developmental processes downstream of the Notch receptor that are critical for specifying unique cell fates, patterning the adult eye and maintaining cellular homeostasis during eye disc morphogenesis.
authors: The Department of Biological Sciences, University of Southern Mississippi, United States.
authors: Sudeshna
authors: S
authors: Das
authors: Q Brent
authors: QB
authors: Chen
authors: Joseph D
authors: JD
authors: Saucier
authors: Brandon
authors: B
authors: Drescher
authors: Yan
authors: Y
authors: Zong
authors: Sarah
authors: S
authors: Morgan
authors: John
authors: J
authors: Forstall
authors: Andrew
authors: A
authors: Meriwether
authors: Randy
authors: R
authors: Toranzo
authors: Sandra M
authors: SM
authors: Leal
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000235:genetics; D051792:Basic Helix-Loop-Helix Proteins / Q000235:genetics / Q000378:metabolism; D019521:Body Patterning / Q000235:genetics; D002470:Cell Survival / Q000235:genetics; D029721:Drosophila Proteins / Q000235:genetics* / Q000378:metabolism; D004331:Drosophila melanogaster / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D005123:Eye / Q000254:growth & development / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D060227:Imaginal Discs / Q000254:growth & development / Q000378:metabolism*; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism; D007814:Larva / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D051379:Mice; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism; D011679:Pupa / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D051880:Receptors, Notch / Q000235:genetics / Q000378:metabolism; D011984:Sensory Receptor Cells / Q000166:cytology / Q000378:metabolism*; D015398:Signal Transduction; D020825:T-Box Domain Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: D051792:Basic Helix-Loop-Helix Proteins; D029721:Drosophila Proteins; D047908:Intracellular Signaling Peptides and Proteins; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D051880:Receptors, Notch; D020825:T-Box Domain Proteins; C497759:N protein, Drosophila; C486013:Tbx20 protein, mouse; C082142:ato protein, Drosophila; C064045:delta protein; C495571:mid protein, Drosophila
keywords: Apoptosis; Delta; Midline; Notch; Sensory Organ Precursor Cell; Tbx20
references: Genes Dev. 1990 Aug;4(8):1322-31
references: 1977661
references: Cell. 1990 Apr 6;61(1):39-48
references: 1690605
references: Science. 1999 Jan 1;283(5398):91-4
references: 9872749
references: Development. 2003 Nov;130(21):5065-72
references: 12975340
references: J Cell Biol. 2006 May 8;173(3):443-52
references: 16682530
references: J Cell Biol. 1991 May;113(3):657-69
references: 2016340
references: Cold Spring Harb Perspect Biol. 2010 Jul;2(7):a001131
references: 20595397
references: Dev Dyn. 2012 Jan;241(1):40-56
references: 21932322
references: Development. 2002 May;129(9):2247-58
references: 11959832
references: Science. 1994 Apr 29;264(5159):677-83
references: 8171319
references: Dev Biol. 2006 Apr 15;292(2):418-29
references: 16499900
references: Development. 1993 Dec;119(4):1277-91
references: 7916679
references: Dev Biol. 1994 Oct;165(2):716-26
references: 7958432
references: Curr Biol. 2004 Oct 5;14(19):1694-702
references: 15458640
references: Development. 1998 Aug;125(15):2893-900
references: 9655811
references: J Neurobiol. 1991 Jul;22(5):443-61
references: 1716300
references: Mech Dev. 1995 Feb;49(3):173-89
references: 7734391
references: Cell. 1995 Sep 22;82(6):1013-23
references: 7553843
references: Genes Dev. 1991 Jun;5(6):996-1008
references: 2044965
references: Genes Dev. 1991 Jun;5(6):984-95
references: 2044964
references: Cell. 1993 Dec 3;75(5):913-26
references: 8252627
references: Genes Dev. 1996 Jul 15;10(14):1773-82
references: 8698237
references: Curr Biol. 2004 Feb 3;14(3):R129-38
references: 14986688
references: Development. 2000 Jun;127(11):2383-93
references: 10804180
references: Genetics. 2005 Nov;171(3):1137-52
references: 16118195
references: Dev Biol. 2012 Nov 1;371(1):110-20
references: 22877946
references: Genetics. 1996 Jul;143(3):1271-86
references: 8807299
references: Genetics. 1995 Dec;141(4):1491-505
references: 8601489
references: Genomics. 2000 Aug 1;67(3):317-32
references: 10936053
references: Development. 2011 Jan;138(2):215-25
references: 21148185
references: Genes Dev. 1994 Nov 15;8(22):2743-55
references: 7958930
references: Development. 1994 Aug;120(8):2121-9
references: 7925015
references: Development. 2008 Dec;135(24):4071-9
references: 19004852
references: BMC Dev Biol. 2005 Mar 10;5:6
references: 15760463
references: Mech Dev. 1999 Feb;80(2):133-46
references: 10072780
references: Nature. 1999 Apr 8;398(6727):518-22
references: 10206645
references: PLoS Biol. 2009 Feb 24;7(2):e40
references: 19243220
references: Curr Opin Neurobiol. 1997 Feb;7(1):48-54
references: 9039792
references: J Embryol Exp Morphol. 1981 Dec;66:57-80
references: 6802923
references: Neuron. 1996 Feb;16(2):275-86
references: 8789943
references: Development. 2005 Jan;132(1):3-13
references: 15590745
references: Development. 1993 Apr;117(4):1223-37
references: 8404527
references: Genes Dev. 1995 Jul 15;9(14):1694-708
references: 7622034
references: Development. 1996 Sep;122(9):2801-12
references: 8787754
references: Nature. 1989 Aug 17;340(6234):531-6
references: 2770860
references: Development. 1991 Nov;113(3):825-39
references: 1821853
references: Development. 2002 Jul;129(13):3269-78
references: 12070100
references: Development. 2009 Aug;136(16):2689-93
references: 19605497
references: Invest Ophthalmol Vis Sci. 2012 Mar 15;53(3):1362-71
references: 22427613
references: Dev Biol. 1989 Dec;136(2):346-62
references: 2511048
references: BMC Dev Biol. 2007 Jul 05;7:82
references: 17612403
references: Philos Trans R Soc Lond B Biol Sci. 2011 Apr 27;366(1568):1204-18
references: 21402581
references: Science. 1995 Apr 14;268(5208):225-32
references: 7716513
references: J Biol Chem. 2000 May 26;275(21):15657-64
references: 10747956
references: Crit Rev Biochem Mol Biol. 2006 Nov-Dec;41(6):339-85
references: 17092823
references: Genes Dev. 1989 Aug;3(8):1099-112
references: 2792755
references: Nature. 1998 May 28;393(6683):382-6
references: 9620803
references: Cell. 1990 Apr 6;61(1):27-38
references: 1690604
references: Development. 1998 Jun;125(12):2327-35
references: 9584131
references: Development. 2001 Dec;128(23):4705-14
references: 11731451
references: Development. 1998 May;125(10):1857-65
references: 9550718
references: Dev Biol. 2009 Jan 1;325(1):138-50
references: 19013145
references: Genes Dev. 2003 Dec 1;17(23):2966-78
references: 14665671
references: Development. 2004 Dec;131(23):5971-80
references: 15539491
references: Genetics. 2008 Dec;180(4):2095-110
references: 18832354
references: Dev Biol. 1994 Dec;166(2):415-30
references: 7813766
references: Development. 1991 Sep;113(1):245-55
references: 1764999
references: Development. 1994 Nov;120(11):3105-17
references: 7720555
references: Dev Biol. 2005 Mar 15;279(2):509-24
references: 15733676
references: Cell Death Differ. 2013 Jan;20(1):108-16
references: 22898807
references: Dev Biol. 2004 May 1;269(1):1-17
references: 15081353
references: J Cell Biol. 1999 Sep 20;146(6):1351-64
references: 10491396
references: Dev Biol. 1987 Sep;123(1):264-75
references: 17985474
references: PLoS Biol. 2010 Dec 14;8(12):e1000566
references: 21179433
references: Genesis. 2004 Apr;38(4):182-94
references: 15083519
references: Development. 1994 Mar;120(3):473-81
references: 8162848
references: Neuron. 1996 Jul;17(1):27-41
references: 8755476
references: Development. 2007 Mar;134(5):825-31
references: 17251265
references: Nature. 1994 Jun 2;369(6479):398-400
references: 8196767
references: Genet Mol Res. 2012 Aug 06;11(3):1997-2002
references: 22911584
references: Oncogene. 1999 Nov 18;18(48):6767-75
references: 10597285
references: Cell. 2000 Aug 4;102(3):349-62
references: 10975525
references: Cell. 1996 Nov 15;87(4):651-60
references: 8929534
references: Development. 2003 Oct;130(19):4719-28
references: 12925597
references: Mech Dev. 1995 Apr;50(2-3):201-16
references: 7619731
references: Development. 2002 Mar;129(6):1295-306
references: 11880339
references: Development. 1996 Dec;122(12):4139-47
references: 9012533
references: Development. 1993 Jun;118(2):401-15
references: 8223268
references: Cell Mol Life Sci. 2013 Oct;70(20):3929-45
references: 23479132
references: Development. 1993 Nov;119(3):855-65
references: 8187644
references: Cell. 1992 May 1;69(3):393-9
references: 1316239
references: J Neurobiol. 1993 Jun;24(6):723-39
references: 8331337
references: Development. 1997 Jan;124(2):261-70
references: 9053303
references: Dev Dyn. 2005 Mar;232(3):585-92
references: 15704118
references: Nat Protoc. 2006;1(6):2635-42
references: 17406519
references: Curr Biol. 2011 Feb 22;21(4):278-87
references: 21276725
references: Dev Biol. 1976 Oct 15;53(2):217-40
references: 825400
references: Mech Dev. 2001 Jan;100(1):87-91
references: 11118890
references: Development. 1991 Dec;113(4):1245-56
references: 1811940
references: Genetics. 1993 Oct;135(2):527-39
references: 8244012
references: Development. 2002 Jul;129(14):3393-402
references: 12091309
references: Development. 1995 Jul;121(7):2019-30
references: 7635049
references: Science. 2000 Apr 7;288(5463):146-9
references: 10753120
references: Development. 1998 Jun;125(11):2031-40
references: 9570768
references: Nature. 1997 Jun 26;387(6636):908-12
references: 9202123
references: Mol Cell Biol. 1996 Mar;16(3):1179-88
references: 8622662
references: Cell. 1992 Sep 18;70(6):911-22
references: 1525829
references: Am J Anat. 1991 Dec;192(4):442-71
references: 1781453
references: Cell. 2011 Nov 11;147(4):881-92
references: 22078884
references: Dev Dyn. 2012 Jan;241(1):136-49
references: 22174084