title: Unique, polyfucosylated glycan-receptor interactions are essential for regeneration of Hydra magnipapillata.
abstract: Cell-cell communications, cell-matrix interactions, and cell migrations play a major role in regeneration. However, little is known about the molecular players involved in these critical events, especially cell surface molecules. Here, we demonstrate the role of specific glycan-receptor interactions in the regenerative process using Hydra magnipapillata as a model system. Global characterization of the N- and O-glycans expressed by H. magnipapillata using ultrasensitive mass spectrometry revealed mainly polyfucosylated LacdiNAc antennary structures. Affinity purification showed that a putative C-type lectin (accession number Q6SIX6) is a likely endogenous receptor for the novel polyfucosylated glycans. Disruption of glycan-receptor interactions led to complete shutdown of the regeneration machinery in live Hydra. A time-dependent, lack-of-regeneration phenotype observed upon incubation with exogenous fuco-lectins suggests the involvement of a polyfucose receptor-mediated signaling mechanism during regeneration. Thus, for the first time, the results presented here provide direct evidence for the role of polyfucosylated glycan-receptor interactions in the regeneration of H. magnipapillata.
authors: Institute for Stem Cell Biology and Regenerative Medicine, NCBS-TIFR , Bangalore, 560065 India.
authors: Sonu
authors: S
authors: Sahadevan
authors: Aristotelis
authors: A
authors: Antonopoulos
authors: Stuart M
authors: SM
authors: Haslam
authors: Anne
authors: A
authors: Dell
authors: Subramanian
authors: S
authors: Ramaswamy
authors: Ponnusamy
authors: P
authors: Babu
mesh_terms: D000818:Animals; D002240:Carbohydrate Sequence; D006829:Hydra / Q000166:cytology* / Q000502:physiology*; D037181:Lectins, C-Type / Q000378:metabolism*; D008969:Molecular Sequence Data; D011134:Polysaccharides / Q000737:chemistry / Q000378:metabolism*; D012038:Regeneration
chemical_list: D037181:Lectins, C-Type; D011134:Polysaccharides