title: Three-dimensional culture using human plasma-medium gel with fragmin/protamine microparticles for proliferation of various human cells.
abstract: Fragmin/protamine microparticles (F/P MPs) have been used as carriers for the preservation and activation of cytokines in human plasma (HP)-Dulbecco's modified Eagle's medium (DMEM) gels. This study investigated a three-dimensional (3D) culture system using an HP-DMEM gel with 0.1 mg/mL F/P MPs and 5 ng/mL FGF-2 for the proliferation of human dermal fibroblast cells (DFCs), human microvascular endothelial cells (MVECs) and human coronary smooth muscle cells (SMCs), or 5 ng/mL interleukin (IL)-3/granulocyte-macrophage colony-stimulating factor (GM-CSF) for a human hematopoietic cell line (TF-1 cells). DFCs, MVECs, SMCs and TF-1 cells grew rapidly under 3D culture conditions using a low-concentration HP (2 %)-DMEM gel with F/P MPs and FGF-2 (for DFCs, MVECs and SMCs) or IL-3/GM-CSF (for TF-1 cells) at doubling times of 22, 23, 25 and 18 h, respectively, without the use of animal serum, compared to under 2D culture conditions using low-concentration human serum (2 %)-DMEM with 5 ng/mL FGF-2 or IL-3/GM-CSF on F/P MP-coated plates at doubling times of approximately 26, 25, 40 and 20 h, respectively.
authors: Research Support Center, Dokkyo Medical University, 880 Kitakobayashi, Mibu, Shimotsuga, 321-0293, Tochigi, Japan, skishi@dokkyomed.ac.jp.
authors: Satoko
authors: S
authors: Kishimoto
authors: Masayuki
authors: M
authors: Ishihara
authors: Makoto
authors: M
authors: Takikawa
authors: Megumi
authors: M
authors: Takikawa
authors: Yuki
authors: Y
authors: Sumi
authors: Shingo
authors: S
authors: Nakamura
authors: Masanori
authors: M
authors: Fujita
authors: Toshinori
authors: T
authors: Sato
authors: Tomoharu
authors: T
authors: Kiyosawa
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