title: A rapid automatic processing platform for bead label-assisted microarray analysis: application for genetic hearing-loss mutation detection.
abstract: Molecular diagnostics using microarrays are increasingly being used in clinical diagnosis because of their high throughput, sensitivity, and accuracy. However, standard microarray processing takes several hours and involves manual steps during hybridization, slide clean up, and imaging. Here we describe the development of an integrated platform that automates these individual steps as well as significantly shortens the processing time and improves reproducibility. The platform integrates such key elements as a microfluidic chip, flow control system, temperature control system, imaging system, and automated analysis of clinical results. Bead labeling of microarray signals required a simple imaging system and allowed continuous monitoring of the microarray processing. To demonstrate utility, the automated platform was used to genotype hereditary hearing-loss gene mutations. Compared with conventional microarray processing procedures, the platform increases the efficiency and reproducibility of hybridization, speeding microarray processing through to result analysis. The platform also continuously monitors the microarray signals, which can be used to facilitate optimization of microarray processing conditions. In addition, the modular design of the platform lends itself to development of simultaneous processing of multiple microfluidic chips. We believe the novel features of the platform will benefit its use in clinical settings in which fast, low-complexity molecular genetic testing is required.
authors: 1CapitalBio Corporation, Beijing, P. R. China.
authors: Jiang
authors: J
authors: Zhu
authors: Xiumei
authors: X
authors: Song
authors: Guangxin
authors: G
authors: Xiang
authors: Zhengde
authors: Z
authors: Feng
authors: Hongju
authors: H
authors: Guo
authors: Danyang
authors: D
authors: Mei
authors: Guohao
authors: G
authors: Zhang
authors: Dong
authors: D
authors: Wang
authors: Keith
authors: K
authors: Mitchelson
authors: Wanli
authors: W
authors: Xing
authors: Jing
authors: J
authors: Cheng
mesh_terms: D057205:Automation, Laboratory / Q000379:methods*; D005820:Genetic Testing / Q000379:methods*; D034381:Hearing Loss / Q000175:diagnosis* / Q000235:genetics*; D057166:High-Throughput Screening Assays / Q000379:methods; D006801:Humans; D046228:Microarray Analysis / Q000379:methods*; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D009154:Mutation; D013048:Specimen Handling / Q000379:methods
keywords: automated platform; microarray; microfluidic chip; mutation detection