title: Biodegradable polycaprolactone (PCL) nanosphere encapsulating superoxide dismutase and catalase enzymes.
abstract: Biodegradable polycaprolactone (PCL) nanosphere encapsulating superoxide dismutase (SOD) and catalase (CAT) were successfully synthesized using double emulsion (w/o/w) solvent evaporation technique. Characterization of the nanosphere using dynamic light scattering, field emission scanning electron microscope, and Fourier transform infrared spectroscopy revealed a spherical-shaped nanosphere in a size range of 812 ± 64 nm with moderate protein encapsulation efficiency of 55.42 ± 3.7 % and high in vitro protein release. Human skin HaCat cells were used for analyzing antioxidative properties of SOD- and CAT-encapsulated PCL nanospheres. Oxidative stress condition in HaCat cells was optimized with exposure to hydrogen peroxide (H2O2; 1 mM) as external stress factor and verified through reactive oxygen species (ROS) analysis using H2DCFDA dye. PCL nanosphere encapsulating SOD and CAT together indicated better antioxidative defense against H2O2-induced oxidative stress in human skin HaCat cells in comparison to PCL encapsulating either SOD or CAT alone as well as against direct supplement of SOD and CAT protein solution. Increase in HaCat cells SOD and CAT activities after treatment hints toward uptake of PCL nanosphere into the human skin HaCat cells. The result signifies the role of PCL-encapsulating SOD and CAT nanosphere in alleviating oxidative stress.
authors: Centre for Environment, Indian Institute of Technology Guwahati, Guwahati, Assam, 781039, India.
authors: Sushant
authors: S
authors: Singh
authors: Abhay Narayan
authors: AN
authors: Singh
authors: Anil
authors: A
authors: Verma
authors: Vikash Kumar
authors: VK
authors: Dubey
mesh_terms: D000328:Adult; D000818:Animals; D001672:Biocompatible Materials / Q000378:metabolism / Q000633:toxicity; D002214:Capsules; D002374:Catalase / Q000494:pharmacology; D002417:Cattle; D002460:Cell Line; D029863:Cicer / Q000201:enzymology; D004337:Drug Carriers / Q000378:metabolism / Q000633:toxicity; D016166:Free Radical Scavengers / Q000494:pharmacology; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D054118:Nanospheres; D018384:Oxidative Stress / Q000187:drug effects; D011091:Polyesters / Q000378:metabolism* / Q000633:toxicity; D012867:Skin / Q000166:cytology; D013482:Superoxide Dismutase / Q000494:pharmacology
chemical_list: D001672:Biocompatible Materials; D002214:Capsules; D004337:Drug Carriers; D016166:Free Radical Scavengers; D011091:Polyesters; C016240:polycaprolactone; D006861:Hydrogen Peroxide; D002374:Catalase; D013482:Superoxide Dismutase