title: SVCT2 vitamin C transporter expression in progenitor cells of the postnatal neurogenic niche.
abstract: Known as a critical antioxidant, recent studies suggest that vitamin C plays an important role in stem cell generation, proliferation and differentiation. Vitamin C also enhances neural differentiation during cerebral development, a function that has not been studied in brain precursor cells. We observed that the rat neurogenic niche is structurally organized at day 15 of postnatal development, and proliferation and neural differentiation increase at day 21. In the human brain, a similar subventricular niche was observed at 1-month of postnatal development. Using immunohistochemistry, sodium-vitamin C cotransporter 2 (SVCT2) expression was detected in the subventricular zone (SVZ) and rostral migratory stream (RMS). Low co-distribution of SVCT2 and βIII-tubulin in neuroblasts or type-A cells was detected, and minimal co-localization of SVCT2 and GFAP in type-B or precursor cells was observed. Similar results were obtained in the human neurogenic niche. However, BrdU-positive cells also expressed SVCT2, suggesting a role of vitamin C in neural progenitor proliferation. Primary neurospheres prepared from rat brain and the P19 teratocarcinoma cell line, which forms neurospheres in vitro, were used to analyze the effect of vitamin C in neural stem cells. Both cell types expressed functional SVCT2 in vitro, and ascorbic acid (AA) induced their neural differentiation, increased βIII-tubulin and SVCT2 expression, and amplified vitamin C uptake.
authors: Laboratory of Neurobiology and Stem Cells, Department of Cellular Biology, Center for Advanced Microscopy CMA BIOBIO, University of Concepción Concepción, Chile.
authors: Patricia
authors: P
authors: Pastor
authors: Pedro
authors: P
authors: Cisternas
authors: Katterine
authors: K
authors: Salazar
authors: Carmen
authors: C
authors: Silva-Alvarez
authors: Karina
authors: K
authors: Oyarce
authors: Nery
authors: N
authors: Jara
authors: Francisca
authors: F
authors: Espinoza
authors: Agustín D
authors: AD
authors: Martínez
authors: Francisco
authors: F
authors: Nualart
keywords: SVCT2; brain; ependymal cells; niche; progenitor; stem cells; vitamin C
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