title: Differentially-expressed genes in Candida albicans exposed to ε-poly-L-lysine.
abstract: Candida albicans is an opportunistic human pathogen whose disinfection is a challenge. ε-Poly-L-lysine (ε-PL), an antagonistic agent, can disrupt cell membranes and inhibit the growth of C. albicans. Genes that were differentially-expressed in response to ε-PL were isolated from C. albicans and identified by suppression subtractive hybridization. Ten subtracted clones, that share >98 % homology with known genes of C. albicans, were isolated. Among these, four genes encoded cell wall-associated proteins. Real-time quantitative PCR and northern blot hybridization suggest that these genes are involved in the response to ε-PL. These findings will help to determine the mechanism of the antimicrobial activity of ε-PL against C. albicans.
authors: State Key Laboratory of Food Science and Technology, Nanchang University, 235 Nanjing East Road, Nanchang, 330047, Jiangxi, People's Republic of China, yeruosong@163.com.
authors: Ruo-Song
authors: RS
authors: Ye
authors: Zhi-Hong
authors: ZH
authors: Zhang
authors: Heng-Yi
authors: HY
authors: Xu
authors: Feng
authors: F
authors: Xu
authors: Zoraida P
authors: ZP
authors: Aguilar
authors: Yong-Hua
authors: YH
authors: Xiong
authors: Zhe-Ling
authors: ZL
authors: Zeng
authors: Hua
authors: H
authors: Wei
mesh_terms: D000935:Antifungal Agents / Q000494:pharmacology*; D015152:Blotting, Northern; D002176:Candida albicans / Q000187:drug effects* / Q000235:genetics* / Q000378:metabolism; D002473:Cell Wall / Q000737:chemistry / Q000378:metabolism; D005656:Fungal Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects*; D005800:Genes, Fungal / Q000235:genetics; D011107:Polylysine / Q000494:pharmacology*; D015203:Reproducibility of Results
chemical_list: D000935:Antifungal Agents; D005656:Fungal Proteins; D011107:Polylysine