title: Colpoda secrete viable Listeria monocytogenes within faecal pellets.
abstract: Transmission electron microscopy was used to demonstrate that co-cultures of the ciliate Colpoda RR (an environmental isolate) and Colpoda MLS-5 (a food processing environment isolate) with the pathogenic Listeria monocytogenes DRDC8 resulted in secretion of faecal pellets containing intact DRDC8 cells. A green fluorescent protein expressing variant of DRDC8 was used in co-cultures to confirm that the pellet-associated bacterial cells were L. monocytogenes. Viability was confirmed by plate counts, and assay of microbial respiratory activity-proved DRDC8 cells present within faecal pellets was metabolically active. Following treatment of faecal pellets secreted by Colpoda RR and MLS-5 with gentamycin and sodium hypochlorite (NaOCl), no loss of viability of the pellet-located DRDC8 cells was observed, indicating that faecal pellet encapsulated DRDC8 cells are resistant to biocidal agents. This work suggests that Colpoda-derived faecal pellets may provide a mechanism for transmission of L. monocytogenes and other pathogenic bacteria. Furthermore, bacteria encapsulated by faecal pellets may be resistant to disinfectants and cleaning agents used in food manufacturing and preparation facilities.
authors: School of Molecular and Biomedical Science, The University of Adelaide, Adelaide, SA, 5005, Australia.
authors: Rethish
authors: R
authors: Raghu Nadhanan
authors: Connor J
authors: CJ
authors: Thomas
mesh_terms: D016798:Ciliophora / Q000382:microbiology*; D018920:Coculture Techniques; D004202:Disinfectants / Q000494:pharmacology*; D005511:Food Handling / Q000379:methods; D005839:Gentamicins / Q000494:pharmacology; D008089:Listeria monocytogenes / Q000187:drug effects*; D050296:Microbial Viability; D046529:Microscopy, Electron, Transmission; D012973:Sodium Hypochlorite / Q000494:pharmacology
chemical_list: D004202:Disinfectants; D005839:Gentamicins; D012973:Sodium Hypochlorite