title: Development of Specific Primer for Tricholoma matsutake.
abstract: In this study, in an effort to develop a method for the molecular detection of Tricholoma matsutake in Korea from other closely related Tricholomataceae, a species-specific PCR primer pair, TmF and TmR, was designed using nuclear ribosomal intertranscribed spacer (ITS) sequences. The DTmF and DTmR sequences were 5'-CCTGACGCCAATCTTTTCA-3' and 5'-GGAGAGCAGACTTGTGAGCA-3', respectively. The PCR primers reliably amplified only the ITS sequences of T. matsutake, and not those of other species used in this study.
authors: Department of Life Science, Graduate School, Dongguk University, Gyeongju, Gyeongbuk 780-714, Korea.
authors: Jang-Han
authors: JH
authors: Kim
authors: Yeong-Hwan
authors: YH
authors: Han
keywords: ITS; Tricholoma matsutake; Tricholomataceae
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