title: Enhancement of antibacterial activity of capped silver nanoparticles in combination with antibiotics, on model gram-negative and gram-positive bacteria.
abstract: The nanoparticles used in this study were prepared from AgNO3 using NaBH4 in the presence of capping agents such as citrate, sodium dodecyl sulfate, and polyvinylpyrrolidone. The formed nanoparticles were characterized with UV-Vis, TEM, and XRD. The generation of silver nanoparticles was confirmed from the appearance of yellow colour and an absorption maximum between 399 and 404 nm. The produced nanoparticles were found to be spherical in shape and polydisperse. For citrate, SDS, and PVP capped nanoparticles, the average particle sizes were 38.3 ± 13.5, 19.3 ± 6.0, and 16.0 ± 4.8 nm, respectively. The crystallinity of the nanoparticles in FCC structure is confirmed from the SAED and XRD patterns. Also, the combined antibacterial activity of these differently capped nanoparticles with selected antibiotics (streptomycin, ampicillin, and tetracycline) was evaluated on model Gram-negative and Gram-positive bacteria, employing disc diffusion assay. The activity of the tested antibiotics was enhanced in combination with all the stabilized nanoparticles, against both the Gram classes of bacteria. The combined effects of silver nanoparticles and antibiotics were more prominent with PVP capped nanoparticles as compared to citrate and SDS capped ones. The results of this study demonstrate potential therapeutic applications of silver nanoparticles in combination with antibiotics.
authors: National Centre for Compositional Characterisation of Materials (NCCCM), Bhabha Atomic Research Centre, Hyderabad 500 062, India.
authors: Aruna Jyothi
authors: AJ
authors: Kora
authors: Lori
authors: L
authors: Rastogi
references: Lett Appl Microbiol. 2009 Feb;48(2):173-9
references: 19141039
references: J Antimicrob Chemother. 2004 Dec;54(6):1019-24
references: 15537697
references: Annu Rev Microbiol. 1996;50:753-89
references: 8905098
references: Nanomedicine. 2013 Jan;9(1):105-10
references: 22633901
references: Adv Drug Deliv Rev. 2005 Jul 29;57(10):1451-70
references: 15950313
references: Nanomedicine. 2010 Feb;6(1):103-9
references: 19447203
references: Adv Colloid Interface Sci. 2009 Jan 30;145(1-2):83-96
references: 18945421
references: Colloids Surf B Biointerfaces. 2010 Jun 1;77(2):219-26
references: 20189779
references: Small. 2008 Jun;4(6):746-50
references: 18528852
references: J Colloid Interface Sci. 2004 Jul 1;275(1):177-82
references: 15158396
references: Appl Environ Microbiol. 2008 Apr;74(7):2171-8
references: 18245232
references: Appl Environ Microbiol. 2007 Mar;73(6):1712-20
references: 17261510
references: Mycobiology. 2012 Mar;40(1):27-34
references: 22783131
references: Nanomedicine. 2012 Jan;8(1):37-45
references: 21703988
references: Chemosphere. 2013 Jan;90(2):410-6
references: 22967928
references: Nanomedicine. 2007 Mar;3(1):95-101
references: 17379174
references: Biomaterials. 2011 Feb;32(5):1264-9
references: 21093906
references: Chem Biol. 2000 Jun;7(6):R127-32
references: 10873842
references: Carbohydr Polym. 2012 Oct 1;90(2):915-20
references: 22840020
references: Environ Sci Technol. 2010 Feb 15;44(4):1260-6
references: 20099802
references: Nano Lett. 2012 Aug 8;12(8):4271-5
references: 22765771
references: Nanomedicine. 2007 Jun;3(2):168-71
references: 17468052