title: Biosynthesis of linoleic acid in Tyrophagus mites (Acarina: Acaridae).
abstract: We report here that Tyrophagus similis and Tyrophagus putrescentiae (Astigmata: Acaridae) have the ability to biosynthesize linoleic acid [(9Z, 12Z)-9, 12-octadecadienoic acid] via a Δ12-desaturation step, although animals in general and vertebrates in particular appear to lack this ability. When the mites were fed on dried yeast enriched with d31-hexadecanoic acid (16:0), d27-octadecadienoic acid (18:2), produced from d31-hexadecanoic acid through elongation and desaturation reactions, was identified as a major fatty acid component of phosphatidylcholines (PCs) and phosphatidylethanolamines (PEs) in the mites. The double bond position of d27-octadecadienoic acid (18:2) of PCs and PEs was determined to be 9 and 12, respectively by dimethyldisulfide (DMDS) derivatization. Furthermore, the GC/MS retention time of methyl 9, 12-octadecadienoate obtained from mite extracts agreed well with those of authentic linoleic acid methyl ester. It is still unclear whether the mites themselves or symbiotic microorganisms are responsible for inserting a double bond into the Δ12 position of octadecanoic acid. However, we present here the unique metabolism of fatty acids in the mites.
authors: Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan.
authors: Takako
authors: T
authors: Aboshi
authors: Nobuhiro
authors: N
authors: Shimizu
authors: Yuji
authors: Y
authors: Nakajima
authors: Yoshiyuki
authors: Y
authors: Honda
authors: Yasumasa
authors: Y
authors: Kuwahara
authors: Hiroshi
authors: H
authors: Amano
authors: Naoki
authors: N
authors: Mori
mesh_terms: D040041:Acaridae / Q000737:chemistry / Q000378:metabolism*; D000818:Animals; D008401:Gas Chromatography-Mass Spectrometry; D019787:Linoleic Acid / Q000096:biosynthesis* / Q000737:chemistry; D015394:Molecular Structure
chemical_list: D019787:Linoleic Acid
keywords: Biosynthesis of linoleic acid; CDL; DMDS; ESI; Essential fatty acids; GC/MS; LC/MS; LPC; LPE; PC; PE; PI; Tyrophagus mites; curved desolvation line; dimethyldisulfide; electron spray ionization; gas chromatography mass spectrometry; liquid chromatography mass spectrometry; lyso phospatidylethanolamine; lysophosphatidylcholine; phosphatidylcholine; phosphatidylethanolamine; phosphatidylinositol; Δ12-Desaturase