title: A novel fexA variant from a canine Staphylococcus pseudintermedius isolate that does not confer florfenicol resistance.
abstract: Transposon Tn558 integrated in the chromosomal radC gene was detected for the first time in Staphylococus pseudintermedius. It carried a novel fexA variant (fexAv) that confers only chloramphenicol resistance. The exporter FexAv exhibited two amino acid substitutions, Gly33Ala and Ala37Val, both of which seem to be important for substrate recognition. Site-directed mutagenesis that reverted the mutated base pairs to those present in the original fexA gene restored the chloramphenicol-plus-florfenicol resistance phenotype.
authors: Biochemistry and Molecular Biology, University of La Rioja, Logroño, Spain.
authors: Elena
authors: E
authors: Gómez-Sanz
authors: Kristina
authors: K
authors: Kadlec
authors: Andrea T
authors: AT
authors: Feßler
authors: Myriam
authors: M
authors: Zarazaga
authors: Carmen
authors: C
authors: Torres
authors: Stefan
authors: S
authors: Schwarz
mesh_terms: D019943:Amino Acid Substitution; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D002701:Chloramphenicol / Q000494:pharmacology*; D002876:Chromosomes, Bacterial; D004251:DNA Transposable Elements; D004285:Dogs; D024881:Drug Resistance, Bacterial / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D026901:Membrane Transport Proteins / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D013203:Staphylococcal Infections / Q000382:microbiology / Q000662:veterinary*; D013210:Staphylococcus / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D013839:Thiamphenicol / Q000031:analogs & derivatives* / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D004251:DNA Transposable Elements; D026901:Membrane Transport Proteins; D002701:Chloramphenicol; C035534:florfenicol; D013839:Thiamphenicol
references: J Antimicrob Chemother. 2009 Dec;64(6):1156-64
references: 19808235
references: Ann N Y Acad Sci. 2011 Dec;1241:82-103
references: 22191528
references: Antimicrob Agents Chemother. 2004 Feb;48(2):615-8
references: 14742219
references: J Clin Microbiol. 2006 Dec;44(12):4444-54
references: 17005735
references: Antimicrob Agents Chemother. 2010 Sep;54(9):3953-5
references: 20585133
references: Antimicrob Agents Chemother. 2011 Oct;55(10):4900-4
references: 21768510
references: Antimicrob Agents Chemother. 2007 Feb;51(2):483-7
references: 17145796
references: Antimicrob Agents Chemother. 2012 Mar;56(3):1485-90
references: 22183168
references: Diagn Microbiol Infect Dis. 2012 Aug;73(4):380-2
references: 22682533
references: Antimicrob Agents Chemother. 2010 Dec;54(12):4978-84
references: 20921317
references: Appl Environ Microbiol. 2011 May;77(9):3052-60
references: 21378035
references: Microb Drug Resist. 2012 Feb;18(1):13-8
references: 21718151
references: Antimicrob Agents Chemother. 2010 Aug;54(8):3475-7
references: 20498309
references: Antimicrob Agents Chemother. 2002 Aug;46(8):2337-43
references: 12121902
references: FEMS Microbiol Rev. 2004 Nov;28(5):519-42
references: 15539072
references: Antimicrob Agents Chemother. 2006 Apr;50(4):1156-63
references: 16569824
references: Environ Sci Technol. 2013 Mar 19;47(6):2892-7
references: 23419160
references: Antimicrob Agents Chemother. 2005 Feb;49(2):813-5
references: 15673776
references: Comp Immunol Microbiol Infect Dis. 2011 Sep;34(5):447-53
references: 21903268
references: Antimicrob Agents Chemother. 2005 Jul;49(7):2965-71
references: 15980376
references: J Antimicrob Chemother. 2010 Apr;65(4):619-25
references: 20164198