title: A new on-axis micro-spectrophotometer for combining Raman, fluorescence and UV/Vis absorption spectroscopy with macromolecular crystallography at the Swiss Light Source.
abstract: The combination of X-ray diffraction experiments with optical methods such as Raman, UV/Vis absorption and fluorescence spectroscopy greatly enhances and complements the specificity of the obtained information. The upgraded version of the in situ on-axis micro-spectrophotometer, MS2, at the macromolecular crystallography beamline X10SA of the Swiss Light Source is presented. The instrument newly supports Raman and resonance Raman spectroscopy, in addition to the previously available UV/Vis absorption and fluorescence modes. With the recent upgrades of the spectral bandwidth, instrument stability, detection efficiency and control software, the application range of the instrument and its ease of operation were greatly improved. Its on-axis geometry with collinear X-ray and optical axes to ensure optimal control of the overlap of sample volumes probed by each technique is still unique amongst comparable facilities worldwide and the instrument has now been in general user operation for over two years.
authors: Paul Scherrer Institut, CH-5232 Villigen, Switzerland.
authors: Guillaume
authors: G
authors: Pompidor
authors: Florian S N
authors: FS
authors: Dworkowski
authors: Vincent
authors: V
authors: Thominet
authors: Clemens
authors: C
authors: Schulze-Briese
authors: Martin R
authors: MR
authors: Fuchs
mesh_terms: D017076:Computer-Aided Design; D018360:Crystallography, X-Ray / Q000295:instrumentation / Q000379:methods; D004867:Equipment Design; D046911:Macromolecular Substances; D015203:Reproducibility of Results; D012984:Software; D013050:Spectrometry, Fluorescence / Q000295:instrumentation / Q000379:methods; D013056:Spectrophotometry, Ultraviolet / Q000295:instrumentation / Q000379:methods; D013059:Spectrum Analysis, Raman / Q000295:instrumentation* / Q000379:methods
chemical_list: D046911:Macromolecular Substances
keywords: Raman spectroscopy; complementary techniques; macromolecular crystallography; micro-spectrophotometry; single-crystal spectroscopy
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