title: Effect of dalteparin on atherosclerotic lesion formation in apolipoprotein E-deficient mice.
abstract: We aimed to investigate whether prolonged treatment with dalteparin could inhibit plaque progression. With C57BL/6J mice as the control, genetically deficient apolipoprotein E (apo E) male mice of C57BL/6J strain (apo E(-/-)) were randomly divided into 3 groups. The model group received no dalteparin, while the other 2 groups received dalteparin at 100 and 200 U/kg d, respectively. The aorta was harvested for hematoxylin and eosin staining to observe plaque formation and for immunohistochemical staining to detect the expression of oxidized low-density lipoprotein receptor 1 (LOX-1). The expression of LOX-1 messenger RNA was detected by reverse transcription polymerase chain reaction, while the expression of LOX-1 protein was detected by Western blotting. Dalteparin decreased aortic plaque volume and inhibited aortic LOX-1 protein expression in apo E(-/-) mice. The effect persisted 4 weeks after dalteparin treatment was discontinued. Dalteparin may inhibit atherosclerotic lesions by downregulating the expression of LOX-1 protein.
authors: Department of Geriatrics, Peking University People's Hospital, Beijing, China.
authors: Lin
authors: L
authors: Su
authors: Department of Geriatrics, Peking University People's Hospital, Beijing, China aooha@hotmail.com.
authors: Qingwen
authors: Q
authors: Zhang
authors: Department of Geriatrics, Peking University People's Hospital, Beijing, China.
authors: Hui
authors: H
authors: Bao
authors: Department of Geriatrics, Peking University People's Hospital, Beijing, China.
authors: Wei
authors: W
authors: Li
authors: Department of Geriatrics, Peking University People's Hospital, Beijing, China.
authors: Yide
authors: Y
authors: Miao
authors: Central Laboratory, Peking University People's Hospital, Beijing, China.
authors: Zheng
authors: Z
authors: Yan
authors: Department of Pathology, Peking University People's Hospital, Beijing, China.
authors: Dingbao
authors: D
authors: Chen
mesh_terms: D000818:Animals; D001057:Apolipoproteins E / Q000172:deficiency* / Q000235:genetics; D050197:Atherosclerosis / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D017985:Dalteparin / Q000494:pharmacology*; D015536:Down-Regulation / Q000187:drug effects; D051379:Mice; D018345:Mice, Knockout; D058226:Plaque, Atherosclerotic / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D051127:Scavenger Receptors, Class E / Q000096:biosynthesis / Q000235:genetics
chemical_list: D001057:Apolipoproteins E; C495138:Olr1 protein, mouse; D051127:Scavenger Receptors, Class E; D017985:Dalteparin
keywords: atherosclerosis; dalteparin; lectin-like oxidized LDL receptor 1