title: Effect of leptin on the gluconeogenesis in calf hepatocytes cultured in vitro.
abstract: We have investigated the effect of leptin on gluconeogenesis in the liver. Hepatocytes were cultured and treated with 0, 2.5, 5, 10, 50, 100 ng/mL of leptin in groups I, II, III, IV, V, and VI, respectively. mRNA expression and enzyme activity of pyruvate carboxylase and phosphoenolpyruvate carboxykinase were determined by real-time fluorescence quantitative RT-PCR and biochemical kits, respectively. Compared with group I, mRNA expression of pyruvate carboxylase and phosphoenolpyruvate carboxykinase in groups III, IV, V, and VI were significantly lower (P < 0.01). Pyruvate carboxylase and phosphoenolpyruvate carboxykinase enzyme activity decreased significantly (P < 0.05) when leptin concentration exceeded 5 and 10 ng/mL, respectively. These results indicate that leptin markedly downregulated mRNA expression and enzyme activity of pyruvate carboxylase and phosphoenolpyruvate carboxykinase in hepatocytes, which suggests that high concentrations of LP inhibit hepatocyte gluconeogenesis, thus making negative energy balance more serious.
authors: College of Animal Science and Veterinary Medicine, Jilin University, 5333 Xi'an Road, Changchun, Jilin, 130062, China.
authors: Yu
authors: Y
authors: Li
authors: Xiaobing
authors: X
authors: Li
authors: Yuxiang
authors: Y
authors: Song
authors: Xiaoxia
authors: X
authors: Shi
authors: Hongyan
authors: H
authors: Ding
authors: Wentao
authors: W
authors: Yang
authors: Zhaoxi
authors: Z
authors: Liu
authors: Hui
authors: H
authors: Chen
authors: Xinwei
authors: X
authors: Li
authors: Jianguo
authors: J
authors: Wang
authors: Guowen
authors: G
authors: Liu
authors: Zhe
authors: Z
authors: Wang
mesh_terms: D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D004789:Enzyme Activation / Q000187:drug effects; D004791:Enzyme Inhibitors / Q000494:pharmacology; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D005943:Gluconeogenesis / Q000187:drug effects*; D022781:Hepatocytes / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D020738:Leptin / Q000494:pharmacology*; D010729:Phosphoenolpyruvate Carboxykinase (GTP) / Q000235:genetics / Q000378:metabolism; D011766:Pyruvate Carboxylase / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism
chemical_list: D004791:Enzyme Inhibitors; D020738:Leptin; D012333:RNA, Messenger; D010729:Phosphoenolpyruvate Carboxykinase (GTP); D011766:Pyruvate Carboxylase
keywords: dairy cows; gluconeogenesis; leptin; phosphor-enolpyruvate carboxykinase; pyruvate carboxylase