title: Establishment of Caenorhabditis elegans SID-1-dependent DNA delivery system in cultured silkworm cells.
abstract: The systemic RNA interference defective-1 (SID-1) can transport double-stranded RNA (dsRNA) into cytosol across the cytoplasmic membrane. We report here that ectopic expression of Caenorhabditis elegans SID-1 allows BmN4 cells to import extracellular plasmid dsDNA into cells via the direct soaking method. Interestingly, BmN4-SID1 cells incorporate dsRNA and plasmid DNA simultaneously. Furthermore, the ectopic SID-1 allows us to establish a stably transformed cell line by the simple soaking method. Our results provide an alternative method for silkworm gene function analysis with low cost and low cell toxicity.
authors: Laboratory of Silkworm Science, Kyushu University Graduate School of Bioresource and Bioenvironmental Sciences, 6-10-1 Hakozaki Higashi-ku, Fukuoka, 812-8581, Japan.
authors: Jian
authors: J
authors: Xu
authors: Kaito
authors: K
authors: Yoshimura
authors: Hiroaki
authors: H
authors: Mon
authors: Zhiqing
authors: Z
authors: Li
authors: Li
authors: L
authors: Zhu
authors: Kazuhiro
authors: K
authors: Iiyama
authors: Takahiro
authors: T
authors: Kusakabe
authors: Jae Man
authors: JM
authors: Lee
mesh_terms: D000818:Animals; D001692:Biological Transport; D012831:Bombyx / Q000166:cytology* / Q000378:metabolism; D017173:Caenorhabditis elegans / Q000235:genetics*; D029742:Caenorhabditis elegans Proteins / Q000502:physiology*; D002478:Cells, Cultured; D018014:Gene Transfer Techniques; D008565:Membrane Proteins / Q000502:physiology*; D010957:Plasmids / Q000235:genetics / Q000378:metabolism; D012330:RNA, Double-Stranded / Q000235:genetics* / Q000378:metabolism; D014162:Transfection
chemical_list: D029742:Caenorhabditis elegans Proteins; D008565:Membrane Proteins; D012330:RNA, Double-Stranded; C452203:SID-1 protein, C elegans
references: Mol Pharm. 2009 Jul-Aug;6(4):1170-9
references: 19492848
references: Science. 2003 Sep 12;301(5639):1545-7
references: 12970568
references: Biochem Biophys Res Commun. 2007 Jun 1;357(2):480-6
references: 17434453
references: Methods Enzymol. 2008;450:253-72
references: 19152864
references: J Insect Sci. 2008;8:1-6
references: 20302524
references: Gene Ther. 2004 May;11(9):765-74
references: 14724668
references: Histochem Cell Biol. 2012 Aug;138(2):201-13
references: 22544351
references: Mol Genet Genomics. 2011 Oct;286(3-4):215-24
references: 21842267
references: Insect Biochem Mol Biol. 2012 Feb;42(2):148-54
references: 22178129
references: J Gene Med. 2000 Mar-Apr;2(2):76-88
references: 10809141
references: RNA. 2011 Jun;17(6):1057-65
references: 21474576
references: Appl Microbiol Biotechnol. 2013 Jul;97(13):5921-31
references: 23467826
references: Mol Cell. 2012 Sep 14;47(5):746-54
references: 22902558
references: Mol Biol Rep. 2012 May;39(5):5575-88
references: 22187347
references: RNA. 2009 Mar;15(3):384-90
references: 19155320
references: RNA Biol. 2012 Jan;9(1):40-6
references: 22293577