title: Poly(ethylene) glycol-capped silver and magnetic nanoparticles: synthesis, characterization, and comparison of bactericidal and cytotoxic effects.
abstract: Silver and magnetic (Fe3O4) nanoparticles have attracted wide attention as novel antimicrobial agents due to their unique chemical and physical properties. In order to study the comparative effects on antibacterial and animal cytotoxicity, Staphylococcus aureus and NIH 3T3 fibroblasts were used, respectively. Both nanoparticles were synthesized via a novel matrix-mediated method using poly(ethylene) glycol. Formation of silver nanoparticles was confirmed by fluorescence and ultraviolet-visible spectroscopic techniques. The poly(ethylene) glycol-coated silver and Fe3O4 nanoparticles were characterized by scanning electron microscope, transmission electron microscope, zeta potential, particle size analysis, Fourier-transform infrared, X-ray diffraction, and X-ray photoelectron spectroscopy. The antimicrobial results indicate that both poly(ethylene) glycol-coated silver and Fe3O4 nanoparticles inhibited S. aureus growth at the concentrations of 5 and 10 µg/mL at all time points without showing any significant cytotoxicity on NIH 3T3 fibroblasts. The particle size of both the poly(ethylene) glycol-coated silver and Fe3O4 nanoparticles dominated in the range 10-15 nm, obtained by particle size analyzer. The poly(ethylene) glycol coating on the particles showed less aggregation of nanoparticles, as observed by scanning electron microscope and transmission electron microscope. The overall obtained results indicated that these two nanoparticles were stable and could be used to develop a magnetized antimicrobial scaffolds for biomedical applications.
authors: Centre for Nano-Biotechnology, School of Bio-Sciences and Technology, VIT University, Vellore, Tamil Nadu, India.
authors: A
authors: A
authors: Mandal
authors: S
authors: S
authors: Sekar
authors: N
authors: N
authors: Chandrasekaran
authors: A
authors: A
authors: Mukherjee
authors: T P
authors: TP
authors: Sastry
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D058185:Magnetite Nanoparticles / Q000737:chemistry* / Q000633:toxicity; D051379:Mice; D050296:Microbial Viability / Q000187:drug effects; D041681:NIH 3T3 Cells; D011092:Polyethylene Glycols / Q000737:chemistry; D012834:Silver / Q000737:chemistry* / Q000494:pharmacology / Q000633:toxicity; D013211:Staphylococcus aureus / Q000187:drug effects
chemical_list: D000900:Anti-Bacterial Agents; D058185:Magnetite Nanoparticles; D012834:Silver; D011092:Polyethylene Glycols
keywords: Magnetic (Fe3O4) nanoparticles; bactericide; cytotoxicity; poly(ethylene) glycol; silver (Ag) nanoparticles