title: The Bruchpilot cytomatrix determines the size of the readily releasable pool of synaptic vesicles.
abstract: Synaptic vesicles (SVs) fuse at a specialized membrane domain called the active zone (AZ), covered by a conserved cytomatrix. How exactly cytomatrix components intersect with SV release remains insufficiently understood. We showed previously that loss of the Drosophila melanogaster ELKS family protein Bruchpilot (BRP) eliminates the cytomatrix (T bar) and declusters Ca(2+) channels. In this paper, we explored additional functions of the cytomatrix, starting with the biochemical identification of two BRP isoforms. Both isoforms alternated in a circular array and were important for proper T-bar formation. Basal transmission was decreased in isoform-specific mutants, which we attributed to a reduction in the size of the readily releasable pool (RRP) of SVs. We also found a corresponding reduction in the number of SVs docked close to the remaining cytomatrix. We propose that the macromolecular architecture created by the alternating pattern of the BRP isoforms determines the number of Ca(2+) channel-coupled SV release slots available per AZ and thereby sets the size of the RRP.
authors: Neurogenetik, Institut für Biologie, Freie Universität Berlin, 14195 Berlin, Germany.
authors: Tanja
authors: T
authors: Matkovic
authors: Matthias
authors: M
authors: Siebert
authors: Elena
authors: E
authors: Knoche
authors: Harald
authors: H
authors: Depner
authors: Sara
authors: S
authors: Mertel
authors: David
authors: D
authors: Owald
authors: Manuela
authors: M
authors: Schmidt
authors: Ulrich
authors: U
authors: Thomas
authors: Albert
authors: A
authors: Sickmann
authors: Dirk
authors: D
authors: Kamin
authors: Stefan W
authors: SW
authors: Hell
authors: Jörg
authors: J
authors: Bürger
authors: Christina
authors: C
authors: Hollmann
authors: Thorsten
authors: T
authors: Mielke
authors: Carolin
authors: C
authors: Wichmann
authors: Stephan J
authors: SJ
authors: Sigrist
mesh_terms: D000818:Animals; D003598:Cytoskeletal Proteins / Q000378:metabolism*; D029721:Drosophila Proteins / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000166:cytology* / Q000378:metabolism*; D013572:Synaptic Vesicles / Q000378:metabolism*
chemical_list: C510146:BRP protein, Drosophila; D003598:Cytoskeletal Proteins; D029721:Drosophila Proteins
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