title: Radiotherapy‑induced Gadd45a impairs lacrimal gland epithelial cell migration and proliferation.
abstract: Radiotherapy‑induced lacrimal gland injury is a serious clinical problem currently lacking satisfactory therapeutic strategies. Exploring the mechanisms underlying secondary injuries caused by radiation may aid in the development of novel targeted medicine. In the current study, growth arrest and DNA damage‑inducible 45 α (Gadd45a), a gene which is upregulated in irradiated skin, was observed as being overexpressed in the irradiated lacrimal gland. Moreover, overexpressed Gadd45a may impair lacrimal gland repair by inhibiting lacrimal gland epithelial cell migration and proliferation. Further signalling pathway analyses indicated that Gadd45a overexpression suppresses Akt (protein kinase B, PKB), P38 and JNK phosphorylation. Thus, the results of the current study suggested that Gadd45a may be a therapeutic target in radiation‑induced lacrimal gland injury.
authors: Department of Plastic Surgery, Shanghai Xi Mei Aesthetic and Plastic Center, Shanghai 200031, P.R. China.
authors: Huiyong
authors: H
authors: Wang
authors: Yanqing
authors: Y
authors: Zhang
authors: Jiang
authors: J
authors: Qian
authors: Mingui
authors: M
authors: Zhang
authors: Xiangning
authors: X
authors: Wang
mesh_terms: D000818:Animals; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism*; D002465:Cell Movement; D049109:Cell Proliferation; D002478:Cells, Cultured; D005260:Female; D015870:Gene Expression; D005786:Gene Expression Regulation / Q000528:radiation effects; D048031:JNK Mitogen-Activated Protein Kinases / Q000378:metabolism; D007765:Lacrimal Apparatus / Q000378:metabolism* / Q000473:pathology / Q000528:radiation effects; D051379:Mice; D008810:Mice, Inbred C57BL; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D010766:Phosphorylation; D011499:Protein Processing, Post-Translational; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D011833:Radiation Injuries, Experimental / Q000378:metabolism*; D015854:Up-Regulation; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism; D000097642:GADD45 Proteins
chemical_list: D018797:Cell Cycle Proteins; D048031:JNK Mitogen-Activated Protein Kinases; D009687:Nuclear Proteins; D051057:Proto-Oncogene Proteins c-akt; D048051:p38 Mitogen-Activated Protein Kinases; C499072:Gadd45a protein, mouse; D000097642:GADD45 Proteins