title: High fat diet induced obesity alters ovarian phosphatidylinositol-3 kinase signaling gene expression.
abstract: Insulin regulates ovarian phosphatidylinositol-3-kinase (PI3 K) signaling, important for primordial follicle viability and growth activation. This study investigated diet-induced obesity impacts on: (1) insulin receptor (Insr) and insulin receptor substrate 1 (Irs1); (2) PI3K components (Kit ligand (Kitlg), kit (c-Kit), protein kinase B alpha (Akt1) and forkhead transcription factor subfamily 3 (Foxo3a)); (3) xenobiotic biotransformation (microsomal epoxide hydrolase (Ephx1), Cytochrome P450 isoform 2E1 (Cyp2e1), Glutathione S-transferase (Gst) isoforms mu (Gstm) and pi (Gstp)) and (4) microRNA's 184, 205, 103 and 21 gene expression. INSR, GSTM and GSTP protein levels were also measured. Obese mouse ovaries had decreased Irs1, Foxo3a, Cyp2e1, MiR-103, and MiR-21 but increased Kitlg, Akt1, and miR-184 levels relative to lean littermates. These results support that diet-induced obesity potentially impairs ovarian function through aberrant gene expression.
authors: Department of Animal Science, Iowa State University, Ames, IA 50011, USA. Electronic address: Nteeba@iastate.edu.
authors: J
authors: J
authors: Nteeba
authors: J W
authors: JW
authors: Ross
authors: J W
authors: JW
authors: Perfield
authors: A F
authors: AF
authors: Keating
mesh_terms: D000818:Animals; D059305:Diet, High-Fat; D005260:Female; D015870:Gene Expression; D007328:Insulin / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D035683:MicroRNAs / Q000378:metabolism; D009765:Obesity / Q000235:genetics / Q000378:metabolism*; D009929:Organ Size; D010053:Ovary / Q000033:anatomy & histology / Q000378:metabolism*; D058539:Phosphatidylinositol 3-Kinase / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D015398:Signal Transduction; D015262:Xenobiotics / Q000378:metabolism
chemical_list: D007328:Insulin; D035683:MicroRNAs; D012333:RNA, Messenger; D015262:Xenobiotics; D058539:Phosphatidylinositol 3-Kinase
keywords: Obesity; Ovary; Phosphatidylinositol-3 kinase
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