title: Rational design and chromatographic evaluation of histamine imprinted polymers optimised for solid-phase extraction of wine samples.
abstract: This article reports on the computational design, development and application of a molecularly imprinted polymer (MIP) with specific affinity towards histamine. Computational modelling was used to screen a monomer library in order to select the monomers able to form the strongest complex with the target analyte. These were subsequently used for MIP synthesis by radical polymerisation initiated by UV. MIPs were then evaluated by liquid chromatography and solid phase extraction (SPE) and best MIP behaviour was observed when itaconic acid was used as functional monomer. Finally, after optimisation of the polymer composition, MIPs were used as adsorbents for SPE and clean-up of histamine in wine samples. The proposed histamine extraction method with the MIP-SPE cartridge was found to be reproducible (<5% RSD) and accurate (93-99% recovery) and provided clear wine extracts. The described methodology is simple and fast and is suitable for the selective histamine extraction and its subsequent quantification by HPLC-DAD from complex matrices such as wine samples.
authors: Department of Analytical Chemistry, Faculty of Pharmacy, University of the Basque Country, 01006 Vitoria-Gasteiz, Spain.
authors: Itsaso
authors: I
authors: Basozabal
authors: Alberto
authors: A
authors: Gomez-Caballero
authors: Goretti
authors: G
authors: Diaz-Diaz
authors: António
authors: A
authors: Guerreiro
authors: Stuart
authors: S
authors: Gilby
authors: M Aranzazu
authors: MA
authors: Goicolea
authors: Ramón J
authors: RJ
authors: Barrio
mesh_terms: D000596:Amino Acids / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003198:Computer Simulation; D006632:Histamine / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*; D057230:Limit of Detection; D054802:Molecular Imprinting / Q000379:methods*; D011108:Polymers / Q000737:chemistry; D015203:Reproducibility of Results; D052616:Solid Phase Extraction / Q000379:methods*; D014920:Wine / Q000032:analysis*
chemical_list: D000596:Amino Acids; D011108:Polymers; D006632:Histamine
keywords: HPLC-DAD; Histamine; MIPs; SPE; Wine