title: Identification and Characterization of Eurotium rubrum Isolated from Meju in Korea.
abstract: We isolated and identified a strain of Eurotium rubrum from Meju that has not been reported in Korea. This fungus is yellowish brown; reverse dark brown on CYA and PDA while yellow on 2% MEA at 25℃. Cleistothecia are first bright yellow and gradually turned brown. Mycerial growth on CYA attained a diameter of 30 mm at 20℃, 37 mm at 25℃ and 32 mm at 30℃ after 15 days. The isolate grew slower on 2% MEA (< 20 mm 15 days at 25℃) compared to CYA and PDA (< 40 mm 15 days at 25℃). Cleistothecia are superficial, yellow to light brown, globose to subglobose, 40~75 µm in diameter. Asci are 8-spored and globose to subglobose 8~11 µm. Ascospores are disciform, 4.0~5.0 µm in length and 4.2~4.5 µm in width. Conidia are ovate or bacillar, finely roughened to densely spinulose, 4.6~6.0 µm in length and 3.0~4.3 µm in width. Compared to known Eurotium rubrum, the Korean isolate showed 99% sequence similarity in ITS rDNA (554 bp) and calmodulin (750 bp) gene and 100% in β-tubulin (1016 bp) gene. The E. rubrum isolate also had weak β-glucosidase and protease activities.
authors: Department of Microbiology, Dankook University, Cheonan 330-714, Korea.
authors: Yeo Hong
authors: YH
authors: Yun
authors: Min Woo
authors: MW
authors: Hyun
authors: Dong Yeon
authors: DY
authors: Suh
authors: Yong Min
authors: YM
authors: Kim
authors: Seong Hwan
authors: SH
authors: Kim
keywords: Calmodulin gene; Eurotium rubrum; ITS rDNA; Meju; β-tubulin gene
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