title: Phosphatidylethanolamine binding protein 1 in vacular endothelial cell autophagy and atherosclerosis.
abstract: We previously found that phosphatidylcholine-specific phospholipase C (PC-PLC) was a key inducing element of atherosclerosis, and might negatively regulate human umbilical vein endothelial cell (HUVEC) autophagy. To further investigate the mechanism of PC-PLC action, we initially identified phosphatidylethanolamine binding protein 1 (PEBP1) as a binding partner of PC-PLC by using mass spectrometry (MS, MALDI-TOF/TOF). We found that PEBP1 positively regulated PC-PLC activity in HUVECs, and inhibition of PC-PLC by its inhibitor D609 suppressed PEBP1 expression dramatically. Moreover, both PC-PLC and PEBP1 negatively regulated HUVEC autophagy independently of mammalian target of rapamycin (mTOR). Furthermore, the PEBP1 level was elevated during the development of atherosclerosis, while D609 significantly decreased the upregulated PEBP1 level in apoE(-/-) mice.
authors: J. Y. Miao and B. X. Zhao: Institute of Developmental Biology, School of Life Science, Shandong University, Jinan 250100, China. Emails: miaojy@sdu.edu.cn and bxzhao@sdu.edu.cn.
authors: Li
authors: L
authors: Wang
authors: HaiYing
authors: H
authors: Li
authors: JinFeng
authors: J
authors: Zhang
authors: Wei
authors: W
authors: Lu
authors: Jing
authors: J
authors: Zhao
authors: Le
authors: L
authors: Su
authors: BaoXiang
authors: B
authors: Zhao
authors: Yun
authors: Y
authors: Zhang
authors: ShangLi
authors: S
authors: Zhang
authors: JunYing
authors: J
authors: Miao
mesh_terms: D000818:Animals; D001057:Apolipoproteins E / Q000235:genetics; D050197:Atherosclerosis / Q000378:metabolism*; D001343:Autophagy; D001952:Bridged-Ring Compounds / Q000494:pharmacology; D061307:Human Umbilical Vein Endothelial Cells / Q000378:metabolism* / Q000502:physiology; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL; D009636:Norbornanes; D049488:Phosphatidylethanolamine Binding Protein / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D058570:TOR Serine-Threonine Kinases / Q000378:metabolism; D013859:Thiocarbamates; D013871:Thiones / Q000494:pharmacology; D010738:Type C Phospholipases / Q000037:antagonists & inhibitors / Q000378:metabolism
chemical_list: D001057:Apolipoproteins E; D001952:Bridged-Ring Compounds; D009636:Norbornanes; C511482:PEBP1 protein, human; D049488:Phosphatidylethanolamine Binding Protein; D013859:Thiocarbamates; D013871:Thiones; C046498:tricyclodecane-9-yl-xanthogenate; D058570:TOR Serine-Threonine Kinases; D010738:Type C Phospholipases; C105598:phosphatidylcholine-specific phospholipase C
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