title: Differential gene expression in peripheral blood T cells from patients with psoriasis, lichen planus, and atopic dermatitis.
abstract: BACKGROUND
  Psoriasis, lichen planus (LP), and atopic dermatitis (AD) are common chronic inflammatory skin diseases mediated by immune responses.
  
  
  OBJECTIVE
  We used RNA sequencing to investigate messenger RNA expression patterns in peripheral T cells of Chinese patients with psoriasis, LP, or AD and of healthy individuals.
  
  
  METHODS
  After peripheral T-cell proliferation, messenger RNA expression patterns were investigated by RNA sequencing, and 6 randomly selected genes were verified by real-time reverse transcriptase polymerase chain reaction.
  
  
  RESULTS
  Six genes were down-regulated and 33 were up-regulated in these diseases. Gene ontology analysis revealed enrichment of genes involved in positive regulation of T-cell activation. Regulation of nuclear premessenger RNA domain containing 1B (RPRD1B) expression was enhanced in psoriasis.
  
  
  CONCLUSIONS
  The role of hereditary factors in RPRD1B expression in T cells was not considered. Immunomodulators (thymopeptide, levamisole, BCG polysaccharide, nucleic acid injection, and transfer factor) were previously given to patients with psoriasis and LP, but not to patients with AD; the effects of these immunomodulators on gene expression is uncertain.
  
  
  CONCLUSIONS
  RPRD1B may be involved in T-cell activation in our Chinese psoriatic cohort, and may play a role in stimulating epidermal hyperproliferation.
authors: Institute of Dermatology, Taiyuan City Center Hospital, Taiyuan, China.
authors: Xinhua
authors: X
authors: Li
authors: Institute of Dermatology, Taiyuan City Center Hospital, Taiyuan, China.
authors: Junqin
authors: J
authors: Li
authors: Institute of Dermatology, Taiyuan City Center Hospital, Taiyuan, China.
authors: Yuanwen
authors: Y
authors: Yang
authors: Institute of Dermatology, Taiyuan City Center Hospital, Taiyuan, China.
authors: Ruixia
authors: R
authors: Hou
authors: Institute of Dermatology, Taiyuan City Center Hospital, Taiyuan, China.
authors: Ruifeng
authors: R
authors: Liu
authors: Institute of Dermatology, Taiyuan City Center Hospital, Taiyuan, China.
authors: Xincheng
authors: X
authors: Zhao
authors: Institute of Dermatology, Taiyuan City Center Hospital, Taiyuan, China.
authors: Xin
authors: X
authors: Yan
authors: Institute of Dermatology, Taiyuan City Center Hospital, Taiyuan, China.
authors: Guohua
authors: G
authors: Yin
authors: Department of Dermatology, Affiliated Hospital of Qinghai University, Xining, China.
authors: Peng
authors: P
authors: An
authors: Department of Dermatology, Affiliated Hospital of Qinghai University, Xining, China.
authors: Yong
authors: Y
authors: Wang
authors: Institute of Dermatology, Taiyuan City Center Hospital, Taiyuan, China. Electronic address: zhangkaiming@sina.com.
authors: Kaiming
authors: K
authors: Zhang
mesh_terms: D000328:Adult; D003876:Dermatitis, Atopic / Q000097:blood / Q000235:genetics*; D005260:Female; D015870:Gene Expression; D006801:Humans; D008010:Lichen Planus / Q000097:blood / Q000235:genetics*; D008297:Male; D011565:Psoriasis / Q000097:blood / Q000235:genetics*; D012333:RNA, Messenger / Q000096:biosynthesis; D013601:T-Lymphocytes
chemical_list: D012333:RNA, Messenger
keywords: AD; CD244; CD30; DEGs; GO; IL; KLRC2; LP; PCR; RGS16; RPRD1B; RT; T cells; atopic dermatitis; cDNA; complementary DNA; differentially expressed genes; gene ontology; interleukin; killer cell lectin-like receptor subfamily C, member 2; lichen planus; natural killer cell receptor 2B4; polymerase chain reaction; psoriasis; regulation of nuclear premessenger RNA domain containing 1B; regulator of G-protein signaling 16; reverse transcriptase; tumor necrosis factor receptor superfamily, member 8