title: Effect of qubi zhentong recipe on synovial gene expression in collagen-induced arthritis in rats.
abstract: OBJECTIVE
  To investigate the effect of the Chinese medical formula Qubi Zhentong Recipe(, QZR) on the synovial gene expression profile in collagen-induced arthritis (CIA) rats.
  
  
  METHODS
  Ten rats were randomly chosen from 60 rats as the control group, and the other 50 rats were used for the CIA models. The CIA model group was constructed by bovine injection of type II collagen through the rats' neck and tail. Twenty rats were randomly chosen from 34 successful CIA models and randomly assigned into two groups: the model group (n =10) and the QZR group (n=10). The QZR group was fed intragastrically with QZR 22.9 g/(kg·d) (10 times the clinical adult dose), and the CIA model group was given the same dose of normal saline. Both model and QZR groups were administered treatment once a day. Total RNA was collected from the knee joint synovium after 30 days. The change in gene expression profile was analyzed by a whole gene chip.
  
  
  RESULTS
  A total of 76 genes showed a difference in expression between CIA model group and the control group; 35 genes were down-regulated and 41 were up-regulated. A total of 67 genes showed a difference in expression between the model group and the QZR group; 48 genes were down-regulated and 19 were upregulated.
  
  
  CONCLUSIONS
  QZR may affect CIA by stimulating multiple genes and targets, which are related to oncogenes, apoptosis, metabolism, the immune system, ion channels, and transport proteins.
authors: Department of Arthropathy, Zhejiang Provincial Hospital of Integrated Traditional and Western Medicine, Hangzhou, 310003, China, hhkjyjm@163.com.
authors: Jian-ming
authors: JM
authors: Yu
authors: Yun-qing
authors: YQ
authors: Wang
authors: Xi-de
authors: XD
authors: Liu
authors: Pi-sheng
authors: PS
authors: Qu
authors: Fan
authors: F
authors: Tao
authors: Qing-ge
authors: QG
authors: Guo
mesh_terms: D000818:Animals; D001169:Arthritis, Experimental / Q000235:genetics*; D002417:Cattle; D004195:Disease Models, Animal; D015536:Down-Regulation / Q000187:drug effects / Q000235:genetics; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D004587:Electrophoresis, Agar Gel; D005121:Extremities / Q000473:pathology; D005786:Gene Expression Regulation / Q000187:drug effects*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D013583:Synovial Membrane / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology*; D059467:Transcriptome; D015854:Up-Regulation / Q000187:drug effects / Q000235:genetics
chemical_list: D004365:Drugs, Chinese Herbal; C000588968:qubi zhentong
references: Orv Hetil. 2010 Jun 20;151(25):1003-10
references: 20519185
references: EMBO J. 1997 Mar 3;16(5):968-77
references: 9118958
references: Nature. 2003 May 15;423(6937):356-61
references: 12748655
references: Immunol Today. 1997 Jan;18(1):44-51
references: 9018974
references: Cell. 1991 Nov 29;67(5):889-99
references: 1959134
references: Nephron Exp Nephrol. 2007;105(4):e117-23
references: 17347581
references: Clin Exp Immunol. 2002 Apr;128(1):44-51
references: 11982589
references: Trends Immunol. 2001 Apr;22(4):199-204
references: 11274925
references: Autoimmunity. 2006 Jun;39(4):307-13
references: 16891219
references: J Biol Chem. 1999 Sep 10;274(37):26523-8
references: 10473614
references: Arthritis Rheum. 2008 Mar;58(3):707-17
references: 18311795
references: Chin J Integr Med. 2013 Jun;19(6):453-8
references: 21717160
references: Br J Cancer. 2008 May 6;98(9):1562-9
references: 18454162
references: J Exp Med. 1977 Sep 1;146(3):857-68
references: 894190