title: Molecular cloning and expression analysis of a dorsal homologue from Eriocheir sinensis.
abstract: Dorsal as a crucial component of Toll signaling pathway, played important roles in induction and regulation of innate immune responses. In this study, we cloned a NF-κB-like transcription factor Dorsal from Eriocheir sinensis and designated it as EsDorsal. The full-length cDNA of EsDorsal was 2493 bp with a 2022-bp open reading frame (ORF) encoding a 673-amino acid protein. This protein contained a 171-residue conserved Rel homology domain (RHD) and a 102-residue Ig-like, plexins and transcription factors domain (IPT). By phylogenetic analysis, EsDorsal was clustered into one group together with other invertebrate Dorsals or NF-κBs, and then clustered with vertebrate NF-κBs. Quantitative real-time polymerase chain reaction (qRT-PCR) analysis results showed that (a) EsDorsal had higher expression level in immune organs; (b) EsDorsal differentially induced after injection of lipopolysaccharides (LPS), peptidoglycan (PG) or zymosan (GLU). Importantly, EsDorsal was more responsive to LPS than GLU and PG. Collectively, EsDorsal was differentially inducibility in response to various PAMPs, suggesting its involvement in a specific innate immune regulation in E. sinensis.
authors: School of Life Science, East China Normal University, Shanghai, China.
authors: Ai-Qing
authors: AQ
authors: Yu
authors: Xing-Kun
authors: XK
authors: Jin
authors: Shuang
authors: S
authors: Li
authors: Xiao-Nv
authors: XN
authors: Guo
authors: Min-Hao
authors: MH
authors: Wu
authors: Wei-Wei
authors: WW
authors: Li
authors: Qun
authors: Q
authors: Wang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003386:Brachyura / Q000235:genetics* / Q000276:immunology; D003001:Cloning, Molecular / Q000379:methods; D018076:DNA, Complementary / Q000235:genetics / Q000276:immunology; D007113:Immunity, Innate / Q000235:genetics / Q000276:immunology; D008969:Molecular Sequence Data; D016328:NF-kappa B / Q000235:genetics* / Q000276:immunology; D010802:Phylogeny; D016415:Sequence Alignment; D014157:Transcription Factors / Q000235:genetics* / Q000276:immunology
chemical_list: D018076:DNA, Complementary; D016328:NF-kappa B; D014157:Transcription Factors
keywords: Chinese mitten crab; Dorsal; Innate immune signaling; PAMPs