title: Radioprotective effect of epicatechin in cultured human fibroblasts and zebrafish.
abstract: Radiation-induced normal cell damage limits the delivery of high-dose radiation to targeted cancer. This study investigated the effect of epicatechin (EC), a minor component of green tea extracts, on radiation-induced cellular damage in vitro in primary cultured human fibroblasts and in vivo in a zebrafish model. Cell viability, proliferation and wound-healing efficacy, mitochondrial membrane potential, and reactive oxygen species (ROS) generation as well as changes in the signaling pathway related to apoptosis were investigated in fibroblasts. The therapeutic effects of EC were explored in a zebrafish model. EC increased clonogenic survival and restored the migration ability of the fibroblasts after irradiation. EC inhibited radiation-induced ROS generation, mitochondrial dysfunction and cell death. EC significantly reduced the expression of p-JNK, p-38, and cleaved caspase-3 compared with their significant increase after radiation treatment. EC attenuated the radiation-induced embryotoxicity in a zebrafish model. These results suggest that EC represents an effective means of reducing cellular damage and facilitating wound healing after radiation exposure.
authors: Department of Otorhinolaryngology - Head and Neck Surgery, National Health Insurance Corporation Ilsan Hospital, 100 Ilsan Street, Ilsandong-Gu, Goyang, 410-719, Korea.
authors: Hyang Ae
authors: HA
authors: Shin
authors: Yoo Seob
authors: YS
authors: Shin
authors: Sung Un
authors: SU
authors: Kang
authors: Jang Hee
authors: JH
authors: Kim
authors: Young-Taek
authors: YT
authors: Oh
authors: Keun Hyung
authors: KH
authors: Park
authors: Bum Hei
authors: BH
authors: Lee
authors: Chul-Ho
authors: CH
authors: Kim
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000502:physiology / Q000528:radiation effects; D002392:Catechin / Q000008:administration & dosage*; D002465:Cell Movement / Q000502:physiology / Q000528:radiation effects; D002470:Cell Survival / Q000502:physiology / Q000528:radiation effects; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004307:Dose-Response Relationship, Radiation; D004625:Embryo, Nonmammalian / Q000502:physiology* / Q000528:radiation effects*; D005347:Fibroblasts / Q000166:cytology / Q000502:physiology* / Q000528:radiation effects*; D006801:Humans; D011837:Radiation-Protective Agents / Q000008:administration & dosage; D017382:Reactive Oxygen Species / Q000378:metabolism; D015027:Zebrafish / Q000196:embryology* / Q000502:physiology*
chemical_list: D011837:Radiation-Protective Agents; D017382:Reactive Oxygen Species; D002392:Catechin
keywords: epicatechin; fibroblast; radiation; radioprotection; zebrafish
references: Mutat Res. 2006 Oct 10;609(1):17-25
references: 16860592
references: Neuroscience. 2011 Dec 29;199:410-20
references: 21946009
references: Oncogene. 2003 Sep 1;22(37):5885-96
references: 12947395
references: J Soc Integr Oncol. 2007 Winter;5(1):11-7
references: 17309809
references: Phytomedicine. 2002 Jan;9(1):15-25
references: 11924759
references: Antiviral Res. 2002 Jan;53(1):19-34
references: 11684313
references: Burns. 2004 Dec;30(8):772-7
references: 15555788
references: Lancet Oncol. 2011 Feb;12(2):127-36
references: 21236730
references: Nat Protoc. 2007;2(2):329-33
references: 17406593
references: Mutat Res. 1986 Apr;173(4):239-44
references: 3513002
references: Food Chem Toxicol. 2002 Dec;40(12):1745-50
references: 12419687
references: Mutat Res. 1995 Feb;326(2):219-25
references: 7529887
references: Int J Radiat Biol. 2010 Sep;86(9):719-31
references: 20670109
references: Hear Res. 2002 Dec;174(1-2):45-54
references: 12433395
references: Nucl Med Biol. 2001 Aug;28(6):735-40
references: 11518657
references: Nat Med. 2012 Jul 06;18(7):1028-40
references: 22772564
references: Mutat Res. 2008 Jan 31;650(1):48-54
references: 18006366
references: Anticancer Res. 1998 Nov-Dec;18(6B):4779-86
references: 9891557
references: Br J Radiol. 2012 Apr;85(1012):313-30
references: 22294702
references: Toxicology. 2003 Jul 15;189(1-2):1-20
references: 12821279
references: J Nutr Biochem. 2005 Mar;16(3):144-9
references: 15741048
references: Am J Pathol. 2012 Apr;180(4):1340-55
references: 22387320
references: Int J Radiat Biol. 2010 Aug;86(8):613-35
references: 20673129
references: Arch Biochem Biophys. 1994 Jan;308(1):278-84
references: 8311465
references: Cancer Treat Rev. 2011 Nov;37(7):511-9
references: 21324605
references: Free Radic Res Commun. 1993;19(4):241-53
references: 7507456
references: J Clin Oncol. 2006 Jun 20;24(18):2873-8
references: 16782926
references: Carcinogenesis. 1994 Nov;15(11):2575-9
references: 7955108
references: Life Sci. 2007 Jul 26;81(7):519-33
references: 17655876
references: Technol Cancer Res Treat. 2013 Apr;12(2):123-30
references: 23098281
references: Apoptosis. 2008 Sep;13(9):1184-94
references: 18670884
references: Radiat Environ Biophys. 2013 Mar;52(1):123-33
references: 23271171
references: Phytother Res. 2005 Jan;19(1):1-22
references: 15799007
references: Toxicol Lett. 2010 Dec 15;199(3):308-16
references: 20883750
references: Oncologist. 2010;15(4):360-71
references: 20413641
references: Curr Opin Otolaryngol Head Neck Surg. 2005 Dec;13(6):343-8
references: 16282762
references: Radiat Res. 2004 Dec;162(6):711-28
references: 15548121
references: Lancet. 2000 Mar 18;355(9208):949-55
references: 10768432
references: N Engl J Med. 2006 Feb 9;354(6):567-78
references: 16467544