title: CAS directly interacts with vinculin to control mechanosensing and focal adhesion dynamics.
abstract: Focal adhesions are cellular structures through which both mechanical forces and regulatory signals are transmitted. Two focal adhesion-associated proteins, Crk-associated substrate (CAS) and vinculin, were both independently shown to be crucial for the ability of cells to transmit mechanical forces and to regulate cytoskeletal tension. Here, we identify a novel, direct binding interaction between CAS and vinculin. This interaction is mediated by the CAS SRC homology 3 domain and a proline-rich sequence in the hinge region of vinculin. We show that CAS localization in focal adhesions is partially dependent on vinculin, and that CAS-vinculin coupling is required for stretch-induced activation of CAS at the Y410 phosphorylation site. Moreover, CAS-vinculin binding significantly affects the dynamics of CAS and vinculin within focal adhesions as well as the size of focal adhesions. Finally, disruption of CAS binding to vinculin reduces cell stiffness and traction force generation. Taken together, these findings strongly implicate a crucial role of CAS-vinculin interaction in mechanosensing and focal adhesion dynamics.
authors: Department of Cell Biology, Faculty of Science, Charles University in Prague, Czech Republic, Vinicna 7, 128 43, Prague, Czech Republic.
authors: Radoslav
authors: R
authors: Janoštiak
authors: Jan
authors: J
authors: Brábek
authors: Vera
authors: V
authors: Auernheimer
authors: Zuzana
authors: Z
authors: Tatárová
authors: Lena A
authors: LA
authors: Lautscham
authors: Tuli
authors: T
authors: Dey
authors: Jakub
authors: J
authors: Gemperle
authors: Rudolf
authors: R
authors: Merkel
authors: Wolfgang H
authors: WH
authors: Goldmann
authors: Ben
authors: B
authors: Fabry
authors: Daniel
authors: D
authors: Rösel
mesh_terms: D020816:Amino Acid Motifs; D000818:Animals; D001696:Biomechanical Phenomena; D002448:Cell Adhesion; D002460:Cell Line; D050719:Crk-Associated Substrate Protein / Q000032:analysis / Q000378:metabolism*; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism; D051416:Focal Adhesion Protein-Tyrosine Kinases / Q000378:metabolism; D022001:Focal Adhesions / Q000378:metabolism* / Q000648:ultrastructure; D051379:Mice; D010455:Peptides / Q000737:chemistry / Q000378:metabolism; D010766:Phosphorylation; D011485:Protein Binding; D060066:Protein Interaction Maps; D016596:Vinculin / Q000032:analysis / Q000378:metabolism*; D018909:src Homology Domains
chemical_list: D050719:Crk-Associated Substrate Protein; D010455:Peptides; D016596:Vinculin; C011083:polyproline; D051416:Focal Adhesion Protein-Tyrosine Kinases
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