title: Innovative technique for the direct determination of proteins in calcified aortic valves.
abstract: Aortal valve mineralization very frequently causes a genesis of aortic stenosis, which is the most often surgically treated heart disease. Hydroxyapatite deposits have been identified as one of the causes leading to the loss of elasticity of the aortic valves. It is known that phosphates/calcium is accumulated in valve tissues during mineralization, but the mechanism of this process remains unclear. The work is focused mainly on the study of protein composition of mineralized aortic valves by nano-liquid chromatography electrospray ionization in a quadrupole orthogonal acceleration time-of-flight mass spectrometry. New methodological approach based on direct enzymatic digestion of proteins contained in hydroxyapatite deposits was developed for the study of pathological processes connected with osteogenesis. Our objectives were to simplify the traditional analytical protocols of sample preparation and to analyze the organic components of the explanted aortic valves for significant degenerative aortic stenosis. The study of aortic valve mineralization on the molecular level should contribute to understanding this process, which should consequently lead to effective prevention as well as to new ways of treatment of this grave disease.
authors: Department of Biochemistry and Microbiology, Institute of Chemical Technology, Technická 5, 166 28, Prague, Czech Republic, coufalou@vscht.cz.
authors: Lucie
authors: L
authors: Coufalova
authors: Stepanka
authors: S
authors: Kuckova
authors: Martina
authors: M
authors: Velcovska
authors: Antonin
authors: A
authors: Zeman
authors: Michal
authors: M
authors: Smid
authors: Martina
authors: M
authors: Havelcova
authors: Radovan
authors: R
authors: Hynek
mesh_terms: D001011:Aorta / Q000737:chemistry* / Q000473:pathology; D001021:Aortic Valve / Q000737:chemistry / Q000473:pathology*; D001024:Aortic Valve Stenosis / Q000378:metabolism* / Q000473:pathology; D001706:Biopsy; D002114:Calcinosis / Q000473:pathology; D017886:Durapatite / Q000737:chemistry; D006801:Humans; D010446:Peptide Fragments / Q000032:analysis*; D011506:Proteins / Q000737:chemistry* / Q000302:isolation & purification; D059748:Proteolysis; D021241:Spectrometry, Mass, Electrospray Ionization; D014357:Trypsin / Q000737:chemistry
chemical_list: D010446:Peptide Fragments; D011506:Proteins; D017886:Durapatite; D014357:Trypsin