title: A comparative study of Toxoplasma gondii seroprevalence in three healthy Chinese populations detected using native and recombinant antigens.
abstract: BACKGROUND
  Toxoplasmosis is one of the most common parasitic zoonoses. The seroprevalence of Toxoplasma gondii infection in humans varies widely worldwide. Detection of Toxoplasma-specific antibodies has been a gold standard method for both epidemiological investigation and clinical diagnosis. Genetic investigation indicated that there is a wide distribution of different genome types or variants of the parasite prevalent in different areas. Thus the reliability of using antigens from parasites of a single genome type for diagnosis and epidemiology purposes needs to be extensively evaluated.
  
  
  METHODS
  In this study, the prevalence of T. gondii infection among 880 clinically healthy individuals in China was systematically tested using crude soluble native antigens and purified recombinant antigens of type I and II T. gondii. The T. gondii-specific IgG and IgM in the sera was further confirmed using commercial Toxoplasmosis Diagnosis Kits and Western blot assays.
  
  
  RESULTS
  The sero-prevalence of T. gondii-specific IgG detected with crude native Type I and type II antigens was 12.2% and 11.3% respectively. Whereas the overall prevalence was more than 20% when combined with the results obtained with recombinant tachyzoite and bradyzoite antigens. There was an obvious variation in immune-recognition of parasite antigens among the individuals studied.
  
  
  CONCLUSIONS
  The general prevalence of anti-T. gondii IgG in the study population was likely much higher than previously reported. The data also suggested that there is more genetic diversity among the T. gondii isolates in China. Further, combination of recombinant antigens with clear immuno-recognition will be able to generate more sensitive diagnostic results than those obtained with crude antigens of T. gondii tachyzoites.
authors: Key Laboratory of Zoonosis, Ministry of Education, Jilin University, Xi'an Da Lu 5333, Changchun, China. cqj@jlu.edu.cn
authors: Xiaojing
authors: X
authors: Sun
authors: Huijun
authors: H
authors: Lu
authors: Boyin
authors: B
authors: Jia
authors: Zhiguang
authors: Z
authors: Chang
authors: Shuai
authors: S
authors: Peng
authors: Jigang
authors: J
authors: Yin
authors: Qijun
authors: Q
authors: Chen
authors: Ning
authors: N
authors: Jiang
mesh_terms: D000913:Antibodies, Protozoan / Q000276:immunology; D000953:Antigens, Protozoan / Q000235:genetics / Q000276:immunology*; D002681:China / Q000453:epidemiology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007074:Immunoglobulin G; D007075:Immunoglobulin M / Q000276:immunology; D008297:Male; D015995:Prevalence; D016036:Seroepidemiologic Studies; D014122:Toxoplasma / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D014123:Toxoplasmosis / Q000175:diagnosis* / Q000453:epidemiology* / Q000276:immunology / Q000469:parasitology
chemical_list: D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D007074:Immunoglobulin G; D007075:Immunoglobulin M
references: BMC Infect Dis. 2010 Jan 07;10:4
references: 20055991
references: BMC Infect Dis. 2013 Jun 24;13:287
references: 23800344
references: J Parasitol. 2010 Oct;96(5):1027-9
references: 20481661
references: Rev Inst Med Trop Sao Paulo. 2003 Jul-Aug;45(4):229-31
references: 14502353
references: Parasit Vectors. 2012 Sep 19;5:205
references: 22992281
references: Microb Pathog. 2013 Jan;54:60-6
references: 23044055
references: Vet Parasitol. 2007 Apr 30;145(3-4):352-6
references: 17267132
references: Rev Inst Med Trop Sao Paulo. 2010 Mar-Apr;52(2):63-8
references: 20464125
references: APMIS. 2010 Aug;118(8):529-42
references: 20666734
references: Parasitol Int. 2009 Jun;58(2):193-5
references: 19567233
references: Ann Ist Super Sanita. 2004;40(1):65-70
references: 15269454
references: Mol Biochem Parasitol. 1995 Jul;73(1-2):253-7
references: 8577335
references: Microbes Infect. 2004 Feb;6(2):164-71
references: 14998514
references: Trans R Soc Trop Med Hyg. 2009 Feb;103(2):162-6
references: 18822439
references: Schizophr Bull. 2007 May;33(3):729-36
references: 17085743
references: Trends Parasitol. 2002 May;18(5):198-201
references: 11983592
references: J Clin Pathol. 1980 Jul;33(7):640-3
references: 7430369
references: Acta Trop. 2010 Oct;116(1):9-14
references: 20452323
references: Biol Psychol. 2003 Jul;63(3):253-68
references: 12853170
references: J Proteomics. 2012 Dec 21;77:423-32
references: 23026549
references: Schizophr Bull. 2007 May;33(3):789-91
references: 17404388
references: Schizophr Bull. 2007 May;33(3):782-8
references: 17387159
references: Mol Microbiol. 1995 Jun;16(6):1221-30
references: 8577255
references: Parasit Vectors. 2013 Apr 05;6:85
references: 23561035
references: Parasit Vectors. 2013 Jun 05;6:163
references: 23735002
references: Lancet. 2004 Jun 12;363(9425):1965-76
references: 15194258
references: Clin Infect Dis. 2001 Mar 1;32(5):842-4
references: 11229859
references: Acta Trop. 2006 Dec;100(3):218-22
references: 17157795
references: J Clin Microbiol. 2004 Apr;42(4):1779-81
references: 15071048
references: J Parasitol. 2009 Apr;95(2):271-4
references: 18922040
references: Int J Infect Dis. 2010 Nov;14(11):e978-81
references: 20843718
references: Rev Panam Salud Publica. 1999 Sep;6(3):157-63
references: 10517092
references: Parasit Vectors. 2013 Jan 16;6(1):15
references: 23324409