title: CpG oligodeoxynucleotide induces bone marrow precursor cells into myeloid-derived suppressor cells.
abstract: Dendritic cells (DCs) and myeloid-derived suppressor cells (MDSCs) perform a number of functions in different immunological settings. In standard in vitro experiments, DCs are produced from mouse bone marrow (BM) cells in the presence of granulocyte-macrophage colony-stimulating factor (GM-CSF) and IL-4. Our previous study demonstrated that BM precursor cells could differentiate into MDSCs when co-cultured with poly (I:C). In the present study, BM precursor cells cultured in GM-CSF and IL-4 were treated with CpG oligodeoxynucleotide (CpG ODN). We observed that Gr1+CD11b+ cells exhibiting MDSC functions accumulated in the co-culture system. A similar phenomenon was also observed in Listeria monocytogenes-infected mice. In conclusion, we demonstrated that prolonged CpG ODN stimulation could inhibit the development of DCs and induce the differentiation of BM precursor cells into MDSCs.
authors: Department of Immunology, National Key Laboratory of Medical Immunology and Institute of Immunology, Second Military Medical University, Shanghai 200433, P.R. China.
authors: Jie
authors: J
authors: Chen
authors: Chengyu
authors: C
authors: Deng
authors: Qingmin
authors: Q
authors: Shi
authors: Jun
authors: J
authors: Jiang
authors: Yongbo
authors: Y
authors: Zhang
authors: Wei
authors: W
authors: Shan
authors: Weimin
authors: W
authors: Sun
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects*; D049109:Cell Proliferation; D018920:Coculture Techniques; D016207:Cytokines / Q000378:metabolism; D003713:Dendritic Cells / Q000378:metabolism; D005260:Female; D008089:Listeria monocytogenes / Q000276:immunology; D008088:Listeriosis / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D022423:Myeloid Cells / Q000378:metabolism*; D023461:Myeloid Progenitor Cells / Q000187:drug effects / Q000502:physiology*; D009838:Oligodeoxyribonucleotides / Q000494:pharmacology*; D010587:Phagocytosis; D010641:Phenotype; D013601:T-Lymphocytes / Q000276:immunology; D051217:Toll-Like Receptor 9 / Q000819:agonists
chemical_list: C526902:CpG ODN 2216; D016207:Cytokines; D009838:Oligodeoxyribonucleotides; D051217:Toll-Like Receptor 9