title: Structural elucidation studies of 15-membered azalide macrocycles using H/D exchange and ESI-MS(n.).
abstract: A comprehensive study of fragmentation pathways of 15-membered azalide macrocycles using electrospray ionisation with multistage mass spectrometry (ESI-MS(n)) is presented in this work. Hydrogen/deuterium (H/D) exchange experiments and high-resolution mass spectrometry were used to investigate the proposed fragmentation pathways. In addition, the fragmentation patterns of sodium adduct ions [M+Na](+) of the macrocycles were also investigated as the presence of an alkali metal interacting with the aglycone ring influences the product-ion spectra.
authors: Fidelta d.o.o., Prilaz baruna Filipovića 29, 10000 Zagreb, Croatia. Electronic address: Dubravka.Gembarovski@glpg.com.
authors: Dubravka
authors: D
authors: Gembarovski
authors: Vesna Gabelica
authors: VG
authors: Marković
authors: Nives
authors: N
authors: Galić
mesh_terms: D017963:Azithromycin / Q000032:analysis / Q000737:chemistry; D003903:Deuterium / Q000032:analysis / Q000737:chemistry; D041961:Deuterium Exchange Measurement / Q000379:methods*; D006859:Hydrogen / Q000032:analysis / Q000737:chemistry; D008672:Metals, Alkali / Q000032:analysis / Q000737:chemistry*; D015394:Molecular Structure; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
chemical_list: D008672:Metals, Alkali; D006859:Hydrogen; D017963:Azithromycin; D003903:Deuterium
keywords: 15-Membered azalide aglycone; ESI-MS(n); Fragmentation; H/D Exchange; Macrolides; Sodium adducts