title: New oil-in-water magnetic emulsion as contrast agent for in vivo magnetic resonance imaging (MRI).
abstract: Nowadays, bio-imaging techniques are widely applied for the diagnosis of various diseased/tumoral tissues in the body using different contrast agents. Accordingly, the advancement in bionanotechnology research is enhanced in this regard. Among contrast agents used, superparamagnetic iron oxide nanoparticles were developed by many researchers and applied for in vive magnetic resonance imaging (MRI). In this study, a new oil-in-water magnetic emulsion was used as contrast agent in MRI, after being characterized in terms of particle size, iron oxide content, magnetic properties and colloidal stability using dynamic light scattering (DLS), thermal gravimetric analysis (TGA), vibrating sample magnetometer (VSM) and zeta potential measurement techniques, respectively. The hydrodynamic size and magnetic content of the magnetic colloidal particles were found to be 250 nm and 75 wt%, respectively. In addition, the used magnetic emulsion possesses superparamagentic properties and high colloidal stability in aqueous medium. Then, the magnetic emulsion was highly diluted and administered intravenously to the Sprague dawley rats to be tested as contrast agent for in vivo MRI. In this preliminary study, MRI images showed significant enhancement in contrast, especially for T2 (relaxation time) contrast enhancement, indicating the distribution of magnetic colloidal nanoparticles within organs, like liver, spleen and kidneys of the Sprague dawley rats. In addition, it was found that 500 microL of the highly diluted magnetic emulsion (0.05 wt%) was found adequate for MRI analysis. This seems to be useful for further investigations especially in theranostic applications of magnetic emulsion.
authors: University of Lyon, F-69622 Lyon, France, University Lyon 1, Villeurbanne, CNRS, UMR 5007, LAGEP-CPE, 43 Boulevarde 11 Novembre 1918, F-69622 Villeurbanne, France.
authors: Naveed
authors: N
authors: Ahmed
authors: Chiraz
authors: C
authors: Jaafar-Maalej
authors: Mohamed Mahmoud
authors: MM
authors: Eissa
authors: Hatem
authors: H
authors: Fessi
authors: Abdelhamid
authors: A
authors: Elaissari
mesh_terms: D000818:Animals; D003287:Contrast Media / Q000138:chemical synthesis*; D004655:Emulsions / Q000138:chemical synthesis; D008279:Magnetic Resonance Imaging / Q000379:methods*; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D053769:Nanocapsules / Q000737:chemistry*; D019301:Oleic Acid / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D051598:Whole Body Imaging / Q000379:methods*
chemical_list: D003287:Contrast Media; D004655:Emulsions; D058185:Magnetite Nanoparticles; D053769:Nanocapsules; D019301:Oleic Acid