title: Silk fibroin/gelatin blend films crosslinked with enzymes for biomedical applications.
abstract: Microbial transglutaminase (mTG) and mushroom tyrosinase (MT) are used to crosslink B. mori silk fibroin/gelatin (SF/G) films. Crosslinked and uncrosslinked SF/G films show no phase separation. The thermal behavior and the conformational structure of SF/G films are strongly affected by blending and enzymatic treatment. Formation of high thermally stable crosslinked macromolecular species is observed, suggesting the occurrence of strong intermolecular interactions between the two polymers as confirmed by FT-Raman spectroscopy. Preliminary in vitro tests show that MT-crosslinked blends with G amounts ≥40% and mTG-crosslinked SF/G 60/40 films support C2C12 cardiomyocyte adhesion and proliferation.
authors: Dipartimento di Scienze Biomediche e Neuromotorie, Università di Bologna Via Belmeloro 8/2, Bologna, I-40126, Italy.
authors: Paola
authors: P
authors: Taddei
authors: Valeria
authors: V
authors: Chiono
authors: Anna
authors: A
authors: Anghileri
authors: Giovanni
authors: G
authors: Vozzi
authors: Giuliano
authors: G
authors: Freddi
authors: Gianluca
authors: G
authors: Ciardelli
mesh_terms: D000364:Agaricus / Q000737:chemistry / Q000201:enzymology; D000818:Animals; D001426:Bacterial Proteins / Q000737:chemistry; D001672:Biocompatible Materials / Q000737:chemistry* / Q000494:pharmacology; D012831:Bombyx; D002460:Cell Line; D003432:Cross-Linking Reagents / Q000737:chemistry*; D005349:Fibroins / Q000737:chemistry*; D005656:Fungal Proteins / Q000737:chemistry; D005780:Gelatin / Q000737:chemistry*; D008422:Materials Testing; D051379:Mice; D008855:Microscopy, Electron, Scanning; D008968:Molecular Conformation; D014442:Monophenol Monooxygenase / Q000737:chemistry*; D032383:Myocytes, Cardiac / Q000166:cytology / Q000187:drug effects; D013059:Spectrum Analysis, Raman; D013302:Streptomyces / Q000737:chemistry / Q000201:enzymology; D013552:Swine; D013718:Tensile Strength; D011503:Transglutaminases / Q000737:chemistry*
chemical_list: D001426:Bacterial Proteins; D001672:Biocompatible Materials; D003432:Cross-Linking Reagents; D005656:Fungal Proteins; D005780:Gelatin; D005349:Fibroins; D014442:Monophenol Monooxygenase; D011503:Transglutaminases
keywords: biopolymers; crosslinking; tissue engineering; transglutaminase; tyrosinase