title: Establishment of the metabolite profile for an Antrodia cinnamomea health food product and investigation of its chemoprevention activity.
abstract: Antrodia cinnamomea is an edible fungus endemic to Taiwan that has been attributed with health promotion benefits. An A. cinnamomea mycelium health food product, which was produced by solid-state culture, was selected as the target for investigation in this study. Fourteen representative metabolites of A. cinnamomea mycelium (EMAC) were selected as index compounds to establish the metabolite profile for evaluation of EMAC product quality. It was also demonstrated that EMAC administration significantly reduced liver inflammation and serum oxidative stress in vivo. 4-Acetylantroquinonol B obtained by a bioactivity-guided fractionation from EMAC was able to not only inhibit LPS-induced nitric oxide formation in macrophages but also protect against ethanol-induced oxidative stress in liver cells. The results suggest this A. cinnamomea product might be a potent antioxidative and anti-inflammatory supplement for chemoprevention.
authors: Graduate Institute of Natural Products, College of Pharmacy, Kaohsiung Medical University , Kaohsiung 807, Taiwan.
authors: Hui-Chun
authors: HC
authors: Wang
authors: Fang-Hua
authors: FH
authors: Chu
authors: Shih-Chang
authors: SC
authors: Chien
authors: Jiunn-Wang
authors: JW
authors: Liao
authors: Han-Wen
authors: HW
authors: Hsieh
authors: Wen-Hsiung
authors: WH
authors: Li
authors: Chin-Chung
authors: CC
authors: Lin
authors: Jei-Fu
authors: JF
authors: Shaw
authors: Yueh-Hsiung
authors: YH
authors: Kuo
authors: Sheng-Yang
authors: SY
authors: Wang
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents; D016588:Anticarcinogenic Agents; D000975:Antioxidants; D055491:Antrodia / Q000737:chemistry* / Q000378:metabolism; D018890:Chemoprevention; D000431:Ethanol / Q000494:pharmacology; D058870:Food, Organic; D006293:Health Promotion; D056945:Hep G2 Cells; D006801:Humans; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D008297:Male; D055442:Metabolome; D051379:Mice; D008813:Mice, Inbred ICR; D025282:Mycelium / Q000737:chemistry*; D009569:Nitric Oxide / Q000096:biosynthesis; D018384:Oxidative Stress / Q000187:drug effects
chemical_list: D000893:Anti-Inflammatory Agents; D016588:Anticarcinogenic Agents; D000975:Antioxidants; D008070:Lipopolysaccharides; D009569:Nitric Oxide; D000431:Ethanol