title: Accumulation of invariant NKT cells into inflamed skin in a novel murine model of nickel allergy.
abstract: Nickel (Ni) can cause delayed-type hypersensitivity reactions, which are thought to be mediated by the accumulation of T cells into inflamed skin. Accumulated T cells at the developmental stages in metal allergy are poorly characterized because a suitable animal model has not been established. To investigate the accumulated T cells in allergic inflamed skin, we generated a novel murine model of Ni-induced allergy. The murine model of Ni allergy was induced by two sensitizations of Ni plus lipopolysaccharide solution into the groin followed by three challenges with Ni solution into the footpad. Here we show that a specific TCR repertoire bearing Vα14Jα18, called natural killer (NK) T cells, was expanded monoclonally in BALB/c or C57BL/6 mice. Accumulation of NKT cells was characterized as CD4(+) or CD4(-)CD8(-) T cells. These results suggested that NKT cells are major pathogenic T cells at the elicitation phase of Ni allergy.
authors: Department of Oral and Maxillofacial Surgery, School of Dental Medicine, Tsurumi University, 2-1-3, Tsrumi, Tsurumi-ku, Yokohama, Kanagawa 230-8501, Japan; Department of Rheumatology and Clinical Immunology, Clinical Research Center for Rheumatology and Allergy, Sagamihara National Hospital, National Hospital Organization, 18-1, Sakura-dai, Minami-ku, Sagamihara, Kanagawa 252-0392, Japan; (c)Department of Oral and Maxillofacial Surgery, Toshiba Rinkan Hospital, 7-9-1, Kamitsuruma, Sagamihara, Kanagawa 252-0385, Japan.
authors: Takanori
authors: T
authors: Eguchi
authors: Kenichi
authors: K
authors: Kumagai
authors: Hiroshi
authors: H
authors: Kobayashi
authors: Hiroaki
authors: H
authors: Shigematsu
authors: Kazutaka
authors: K
authors: Kitaura
authors: Satsuki
authors: S
authors: Suzuki
authors: Tatsuya
authors: T
authors: Horikawa
authors: Yoshiki
authors: Y
authors: Hamada
authors: Kouetsu
authors: K
authors: Ogasawara
authors: Ryuji
authors: R
authors: Suzuki
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D006968:Hypersensitivity, Delayed / Q000139:chemically induced / Q000235:genetics / Q000276:immunology*; D007150:Immunohistochemistry; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D055611:Natural Killer T-Cells / Q000187:drug effects / Q000276:immunology*; D009532:Nickel / Q000276:immunology / Q000633:toxicity*; D012333:RNA, Messenger / Q000737:chemistry / Q000235:genetics; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000235:genetics / Q000276:immunology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017443:Skin Diseases, Eczematous / Q000139:chemically induced / Q000235:genetics / Q000276:immunology*
chemical_list: D012333:RNA, Messenger; D016693:Receptors, Antigen, T-Cell, alpha-beta; D009532:Nickel
keywords: Hypersensitivity; IHC; MCA; MCB; Natural killer T cells; Nickel; T-cell receptor; TCR V region; TCR α-chain C region-specific primer; TCR α-chain variable; TCR β-chain C region-specific primer; TCR β-chain variable; TCRAV; TCRBV; TCRV; immunohistochemical