title: Downregulation of microRNA-214 and overexpression of FGFR-1 contribute to hepatocellular carcinoma metastasis.
abstract: miR-214 is one of the most significantly downregulated microRNAs (miRNAs) in hepatocellular carcinoma (HCC). Fibroblast growth factor receptor 1 (FGFR-1) is a miR-214 target gene implicated in the progression of HCC. However, the roles of miR-214 and FGFR-1 in HCC are not fully understood. Here, we analyzed the expression of miR-214 and FGFR-1 in 65 cases of HCC and paired non-neoplastic tissue specimens using real-time PCR and Western blot (WB), respectively. Our data indicated that miR-214 was downregulated and FGFR-1 was overexpressed in HCC compared to the paired non-neoplastic tissues. The low miR-214 expression was correlated with portal vein invasion (p=0.016) and early recurrence (p=0.045) in HCC patients. Moreover, the low miR-214 expression was correlated with high positive rate of FGFR-1 in HCC cases (p=0.020). Our data further demonstrated that miR-214 overexpression in SK-HEP1 and HepG2 cells downregulated FGFR-1 expression and inhibited liver cancer cell invasion. The Luciferase assay results further demonstrated the targeted regulation of FGFR-1 by miR-214. In conclusion, our data indicate that the downregulation of miR-214 in HCC and the upregulation of its target gene FGFR-1 is associated with HCC progression. Therefore, miR-214 and FGFR-1 are potential prognostic markers and therapeutic targets in HCC.
authors: Department of 4th Abdominal Oncology, Tianjin Cancer Institute and Hospital, Tianjin Medical University, Tianjin 300060, China. Electronic address: jianwangtianjin@gmail.com.
authors: Jian
authors: J
authors: Wang
authors: Jingwu
authors: J
authors: Li
authors: Xiuchao
authors: X
authors: Wang
authors: Chen
authors: C
authors: Zheng
authors: Weidong
authors: W
authors: Ma
mesh_terms: D006528:Carcinoma, Hepatocellular / Q000378:metabolism* / Q000473:pathology; D018450:Disease Progression; D018572:Disease-Free Survival; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D056945:Hep G2 Cells; D006801:Humans; D008113:Liver Neoplasms / Q000378:metabolism* / Q000473:pathology; D008297:Male; D035683:MicroRNAs / Q000378:metabolism*; D008875:Middle Aged; D009362:Neoplasm Metastasis; D011379:Prognosis; D051496:Receptor, Fibroblast Growth Factor, Type 1 / Q000378:metabolism*; D012008:Recurrence; D014162:Transfection
chemical_list: C547397:MIRN214 microRNA, human; D035683:MicroRNAs; D051496:Receptor, Fibroblast Growth Factor, Type 1
keywords: FGFR-1; Hepatocellular carcinoma; Metastasis; miR-214