title: A universal DNA-based protein detection system.
abstract: Protein immune detection requires secondary antibodies which must be carefully selected in order to avoid interspecies cross-reactivity, and is therefore restricted by the limited availability of primary/secondary antibody pairs. Here we present a versatile DNA-based protein detection system using a universal adapter to interface between IgG antibodies and DNA-modified reporter molecules. As a demonstration of this capability, we successfully used DNA nano-barcodes, quantum dots, and horseradish peroxidase enzyme to detect multiple proteins using our DNA-based labeling system. Our system not only eliminates secondary antibodies but also serves as a novel method platform for protein detection with modularity, high capacity, and multiplexed capability.
authors: Department of Biological & Environmental Engineering, ∥Department of Molecular Biology and Genetics, and ⊥Kavli Institute at Cornell for Nanoscale Science, Cornell University , Ithaca, New York 14853, United States.
authors: Thua N N
authors: TN
authors: Tran
authors: Jinhui
authors: J
authors: Cui
authors: Mark R
authors: MR
authors: Hartman
authors: Songming
authors: S
authors: Peng
authors: Hisakage
authors: H
authors: Funabashi
authors: Faping
authors: F
authors: Duan
authors: Dayong
authors: D
authors: Yang
authors: John C
authors: JC
authors: March
authors: John T
authors: JT
authors: Lis
authors: Haixin
authors: H
authors: Cui
authors: Dan
authors: D
authors: Luo
mesh_terms: D004247:DNA / Q000737:chemistry*; D005456:Fluorescent Dyes; D006735:Horseradish Peroxidase / Q000737:chemistry; D007074:Immunoglobulin G / Q000737:chemistry*; D017404:In Situ Hybridization, Fluorescence; D007202:Indicators and Reagents; D049329:Nanostructures; D009841:Oligonucleotides / Q000737:chemistry; D011506:Proteins / Q000032:analysis* / Q000276:immunology; D045663:Quantum Dots
chemical_list: D005456:Fluorescent Dyes; D007074:Immunoglobulin G; D007202:Indicators and Reagents; D009841:Oligonucleotides; D011506:Proteins; D004247:DNA; D006735:Horseradish Peroxidase
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