title: Chronological production of thioacetamide-induced cirrhosis in the rat with no mortality.
abstract: BACKGROUND
  Cirrhotic animal models are useful in studying complications of chronic liver disease. The authors chronologically investigated the effect of thioacetamide (TAA), administered intraperitoneally and adapted individually to weight changes, focusing on the optimal moment to obtain typical features of cirrhosis.
  
  
  METHODS
  Male Wistar Rats,150-200 g, were intoxicated three times per week with TAA of 200 mg/kg for 4, 8, 12 or 16 weeks (n = 8 per group), respectively and compared with age-matched controls (n = 4 per group). The individual body weight and liver function test were also measured in each group. Liver samples from each group were histologically stained with Sirius red in order to identify the degree of liver fibrosis.
  
  
  RESULTS
  Rats intoxicated for 4, 8, 12 or 16 weeks had no mortality and histologically showed hepatitis and advanced fibrosis. At 12 and 16 weeks, all animals showed macronodular cirrhosis with signs of high-grade hepatocellular dysplasia. The weight of the treated groups at different time points was significantly lower than the controls. Routine liver function tests between cirrhotic and control rats showed significantly higher only in alanine transaminase (ALT) and aspartate aminotransferase (AST) at 8 and 12 weeks. However in the cirrhotic rats at 16 weeks, the ALT and AST were much lower than that at 8 and 12 weeks but did not show any difference from the controls.
  
  
  CONCLUSIONS
  Thioacetamide, adapted to individual weight changes, leads to a model of cirrhosis in the rat at 12 and 16 weeks with zero mortality.
authors: Medical Diagnostic Department, Thammasat University Hospital, Thammasat University (Rangsit Campus), Pathumthani, Thailand. joearty04@gmail.com
authors: Arthit
authors: A
authors: Norasingha
authors: Wisuit
authors: W
authors: Pradidarcheep
authors: Kanokporn
authors: K
authors: Chayaburakul
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D008106:Liver Cirrhosis, Experimental / Q000139:chemically induced* / Q000401:mortality; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D013853:Thioacetamide / Q000009:adverse effects
chemical_list: D013853:Thioacetamide