title: Synthesis, characterization, DNA binding studies, photocleavage, cytotoxicity and docking studies of ruthenium(II) light switch complexes.
abstract: A new ligand 3-(1H-imidazo[4,5-f][1,10]phenanthrolin-2yl)phenylboronic acid and its (IPPBA) three ruthenium(II) complexes [Ru(phen)2(IPPBA)](ClO4)2 (1), [Ru(bpy)2(IPPBA)](ClO4)2 (2) and [Ru(dmb)2(IPPBA)](ClO4)2 (3) have been synthesized and characterized by elemental analysis, UV/VIS, IR, (1)H-NMR,(13)C-NMR and mass spectra. The binding behaviors of the three complexes to calf thymus DNA were investigated by absorption spectra, emission spectroscopy, viscosity measurements, thermal denaturation and photoactivated cleavage. The DNA-binding constants for complexes 1, 2 and 3 have been determined to be 7.9 × 10(5) M(-1), 6.7 × 10(5) M(-1) and 2.9 × 10(5) M(-1). The results suggest that these complexes bound to double-stranded DNA in an intercalation mode. Upon irradiation at 365 nm, three ruthenium complexes were found to promote the cleavage of plasmid pBR322 DNA from super coiled form І to nicked form ІІ. Further in the presence of Co(2+), the emission of DNA-Ru(ΙΙ) complexes can be quenched. And when EDTA was added, the emission was recovered. The experimental results show that all three complexes exhibited the "on-off-on" properties of molecular "light switch". The highest Cytotoxicity potential of the complex1 was observed on the Human alveolar adenocarcinoma (A549) cell line. Good agreement was generally found between the spectroscopic techniques and molecular docked model which provides further evidence of groove binding.
authors: Department of Chemistry, Osmania University, Hyderabad, Andhra Pradesh, PIN-500007, India.
authors: Nazar Mohammed
authors: NM
authors: Gabra
authors: Bakheit
authors: B
authors: Mustafa
authors: Yata Praveen
authors: YP
authors: Kumar
authors: C Shobha
authors: CS
authors: Devi
authors: A
authors: A
authors: Srishailam
authors: P Venkat
authors: PV
authors: Reddy
authors: Kotha Laxma
authors: KL
authors: Reddy
authors: S
authors: S
authors: Satyanarayana
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D000970:Antineoplastic Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects*; D001665:Binding Sites; D002417:Cattle; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004247:DNA / Q000737:chemistry*; D004305:Dose-Response Relationship, Drug; D004354:Drug Screening Assays, Antitumor; D006367:HeLa Cells; D006801:Humans; D061986:MCF-7 Cells; D008826:Microbial Sensitivity Tests; D008958:Models, Molecular; D015394:Molecular Structure; D009942:Organometallic Compounds / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D055668:Photochemical Processes; D012428:Ruthenium / Q000737:chemistry*; D013329:Structure-Activity Relationship; D014466:Ultraviolet Rays
chemical_list: D000900:Anti-Bacterial Agents; D000970:Antineoplastic Agents; D009942:Organometallic Compounds; D012428:Ruthenium; D004247:DNA; C427203:calf thymus DNA
references: J Inorg Biochem. 2005 Feb;99(2):513-20
references: 15621284
references: Bioconjug Chem. 2008 Apr;19(4):899-910
references: 18324756
references: Science. 1986 Aug 15;233(4765):727-34
references: 3016894
references: Biochemistry. 1993 Mar 16;32(10):2573-84
references: 8448115
references: J Mol Biol. 1995 Jan 6;245(1):43-53
references: 7823319
references: J Mol Biol. 1997 Apr 4;267(3):727-48
references: 9126849
references: Angew Chem Int Ed Engl. 2001 Mar 2;40(5):879-884
references: 11241634
references: Biomaterials. 2013 Jan;34(1):171-80
references: 23059005
references: Bioconjug Chem. 2009 Mar 18;20(3):447-59
references: 19236020
references: Biochemistry. 1982 Aug 17;21(17):3927-32
references: 7126523
references: Biometals. 2010 Feb;23(1):99-118
references: 19787298
references: J Mol Biol. 1965 Aug;13(1):269-82
references: 5859041
references: Biochemistry. 1995 Nov 21;34(46):15049-56
references: 7578117
references: Steroids. 2007 Mar;72(3):247-60
references: 17280694
references: J Inorg Biochem. 2006 Sep;100(9):1487-94
references: 16766033
references: Inorg Chem. 2001 Sep 10;40(19):5045-50
references: 11531457
references: Biochemistry. 2008 Apr 1;47(13):4119-28
references: 18324782
references: J Immunol Methods. 1983 Dec 16;65(1-2):55-63
references: 6606682
references: Biochemistry. 1973 Oct 9;12(21):4161-70
references: 4795686
references: J Inorg Biochem. 2004 Dec;98(12):2011-5
references: 15541489
references: Chem Biodivers. 2006 Nov;3(11):1219-29
references: 17193235
references: J Inorg Biochem. 1989 Dec;37(4):271-82
references: 2628546
references: Biochemistry. 1995 Nov 21;34(46):15037-48
references: 7578116
references: Methods Enzymol. 1991;208:291-343
references: 1779839
references: J Fluoresc. 2012 May;22(3):835-47
references: 22194001