title: MicroRNA-143 downregulates interleukin-13 receptor alpha1 in human mast cells.
abstract: MicroRNA-143 (miR-143) was found to be downregulated in allergic rhinitis, and bioinformatics analysis predicted that IL-13Rα1 was a target gene of miR-143. To understand the molecular mechanisms of miR-143 involved in the pathogenesis of allergic inflammation, recombinant miR-143 plasmid vectors were constructed, and human mast cell-1(HMC-1) cells which play a central role in the allergic response were used for study. The plasmids were transfected into HMC-1 cells using a lentiviral vector. Expression of IL-13Rα1 mRNA was then detected by reverse transcriptase polymerase chain reaction (RT-PCR) and Western Blotting. The miR-143 lentiviral vector was successfully stably transfected in HMC-1 cells for target gene expression. Compared to the control, the target gene IL-13Rα1 was less expressed in HMC-1 transfected with miR-143 as determined by RT-PCR and Western Blotting (p < 0.05); this difference in expression was statistically significant and the inhibition efficiency was 71%. It indicates that miR-143 directly targets IL-13Rα1 and suppresses IL-13Rα1 expression in HMC-1 cells. Therefore, miR-143 may be associated with allergic reaction in human mast cells.
authors: Department of Otolaryngology, Huadong Hospital, Fudan University, Shanghai 200040, China. yu_shaoqing@163.com
authors: Shaoqing
authors: S
authors: Yu
authors: Ruxin
authors: R
authors: Zhang
authors: Chunshen
authors: C
authors: Zhu
authors: Jianqiu
authors: J
authors: Cheng
authors: Hong
authors: H
authors: Wang
authors: Jing
authors: J
authors: Wu
mesh_terms: D001483:Base Sequence; D001665:Binding Sites; D015536:Down-Regulation; D057809:HEK293 Cells; D006801:Humans; D006967:Hypersensitivity / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D053661:Interleukin-13 Receptor alpha1 Subunit / Q000235:genetics* / Q000378:metabolism; D008407:Mast Cells / Q000378:metabolism*; D035683:MicroRNAs / Q000235:genetics*; D034622:RNA Interference
chemical_list: D053661:Interleukin-13 Receptor alpha1 Subunit; C542359:MIRN143 microRNA, human; D035683:MicroRNAs
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