title: Antigenic Peptides Capable of Inducing Specific Antibodies for Detection of the Major Alterations Found in Type 2B Von Willebrand Disease.
abstract: Von Willebrand disease (VWD) is an inherited hemorrhagic disorder promoted by either quantitative or qualitative defects of the von Willebrand factor (VWF). The disease represents the most common human coagulopathy afflicting 1.3% of the population. Qualitative defects are subdivided into four subtypes and classified according to the molecular dysfunction of the VWF. The differential diagnosis of the VWD is a difficult task, relying on a panel of tests aimed to assess the plasma levels and function of the VWF. Here, we propose biochemical approaches for the identification of structural variants of the VWF. A bioinformatic analysis was conducted to design seven peptides among which three were representatives of specific amino acid sequences belonging to normal VWF and four encompassed sequences found in the most common VWD subtype 2B. These peptides were used to immunize mice, after which, peptide-specific immunoglobulins were purified. This resulted in four Ig preparations capable of detecting alterations in the subtype 2B VWD plus additional three antibody fractions targeting the normal VWF. The panel of antibodies could serve many applications among them (1) assessment of VWF: antigen interaction, (2) VWF multimer analysis, and (3) production of monoclonal antibodies against VWF for therapeutic purposes as in thrombotic thrombocytopenic purpura.
authors: Departamento de Ciências Biológicas/DECBI, Instituto de Ciências Exatas e Biológicas/ICEB, Universidade Federal de Ouro Preto, Núcleo de Pesquisas em Ciências Biológicas/NUPEB, Campus Universitário Morro do Cruzeiro, 35400-000 Ouro Preto, MG, Brazil.
authors: Marina de Oliveira
authors: Mde O
authors: Paro
authors: Cyntia Silva
authors: CS
authors: Ferreira
authors: Fernanda Silva
authors: FS
authors: Vieira
authors: Marcos Aurélio
authors: MA
authors: de Santana
authors: William
authors: W
authors: Castro-Borges
authors: Maria Sueli Silva
authors: MS
authors: Namen-Lopes
authors: Sophie Yvette
authors: SY
authors: Leclercq
authors: Cibele
authors: C
authors: Velloso-Rodrigues
authors: Milton Hércules
authors: MH
authors: Guerra de Andrade
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