title: Chemical and behavioral analysis of the cuticular hydrocarbons from Asian citrus psyllid, Diaphorina citri.
abstract: Huanglongbing (HLB) is the most destructive disease of citrus worldwide. The Asian citrus psyllid, Diaphorina citri Kuwayama (Hemiptera: Psyllidae), is the vector of the phloem-inhabiting bacterium, Candidatus Liberibacter asiaticus, which is presumed to cause HLB in Florida citrus. Laboratory and field studies were conducted to examine the behavioral responses of male and female D. citri to their cuticular extracts. In olfactometer assays, more male D. citri were attracted to one, five, or 10 female cuticular extract equivalent units than blank controls. The results were confirmed in field studies in which clear or yellow traps baited with 10 female cuticular extract equivalent units attracted proportionately more males than clear traps baited with male cuticular extract or unbaited traps. Analyses of cuticular constituents of male and female D. citri revealed differences between the sexes in chemical composition of their cuticular extracts. Laboratory bioassays with synthetic chemicals identified from cuticular extracts indicated that dodecanoic acid attracted more males than clean air. Traps baited with dodecanoic acid did not increase total catch of D. citri as compared with blank traps at the dosages tested; however, the sex ratio of psyllid catch was male biased on traps baited with the highest lure loading dosage tested (10.0 mg).
authors: Entomology and Nematology Department, Citrus Research and Education Center, University of Florida, USA.
authors: Rajinder S
authors: RS
authors: Mann
authors: Russell L
authors: RL
authors: Rouseff
authors: Jack
authors: J
authors: Smoot
authors: Nandikeswara
authors: N
authors: Rao
authors: Wendy L
authors: WL
authors: Meyer
authors: Stephen L
authors: SL
authors: Lapointe
authors: Paul S
authors: PS
authors: Robbins
authors: Dong
authors: D
authors: Cha
authors: Charles E
authors: CE
authors: Linn
authors: Francis X
authors: FX
authors: Webster
authors: Siddharth
authors: S
authors: Tiwari
authors: Lukasz L
authors: LL
authors: Stelinski
mesh_terms: D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects*; D005260:Female; D006430:Hemiptera / Q000737:chemistry* / Q000378:metabolism*; D006838:Hydrocarbons / Q000737:chemistry* / Q000494:pharmacology*; D034582:Integumentary System; D008297:Male; D012724:Sex Attractants / Q000737:chemistry / Q000494:pharmacology
chemical_list: D006838:Hydrocarbons; D012724:Sex Attractants
keywords: Huanglongbing; citrus; cuticular hydrocarbon; dodecanoic acid; semiochemical