title: Cytokine responses to quantiferon peptides in pediatric tuberculosis: a pilot study.
abstract: OBJECTIVE
  Detailed understanding of tuberculosis (TB) immunopathology and cytokine/chemokine responses can ultimately lead to the development of new diagnostic tools, especially useful in children where TB diagnosis remains challenging.
  
  
  METHODS
  Nineteen cytokine/chemokine responses to Mycobacterium tuberculosis (M.tb) antigens were analyzed in 47 children distributed as follow: 28 with TB-disease (TD), 12 with latent TB and 7 uninfected controls. All the cytokines and chemokines were quantified in a multiplexed microsphere-based assay by using residual plasma from the quantiFERON kit (IFNγ release assay).
  
  
  RESULTS
  IP-10, IL-2, IL-5 and IL-13 were among the best cytokines to diagnose infection as related by the area under ROC curve for IP-10 (0.96, 95%CI: 0.91-1.00), IL-2 (0.98, 95%CI: 0.93-1.02), IL-5 (0.91, 95%CI: 0.81-1.01) and IL-13 (0.97, 95%CI: 0.93-1.00). None of the 5 biomarkers, however, discriminated TB-disease from latent-TB. Finally, lower IL-5 (p = 0.02) and IL-13 (p = 0.02) levels were observed in severe opposed to non-severe TB.
  
  
  CONCLUSIONS
  These results suggest that IP-10, IL-2, IL-5 and IL-13 may find a diagnostic application in pediatric tuberculosis and argue against the paradigm of a negative influence of Th2 responses in severe pediatric M.tb infection.
authors: Laboratory of Immunology, Robert Debré Hospital, Assistance Publique-Hôpitaux de Paris (AP-HP), Univ. Paris Diderot, Sorbonne Paris Cité, 75019 Paris, France.
authors: Marine
authors: M
authors: Armand
authors: Vibol
authors: V
authors: Chhor
authors: Agathe
authors: A
authors: de Lauzanne
authors: Valérie
authors: V
authors: Guérin-El Khourouj
authors: Béatrice
authors: B
authors: Pédron
authors: Mohamed
authors: M
authors: Jeljeli
authors: Pierre
authors: P
authors: Gressens
authors: Albert
authors: A
authors: Faye
authors: Ghislaine
authors: G
authors: Sterkers
mesh_terms: D000293:Adolescent; D000942:Antigens, Bacterial / Q000276:immunology*; D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D016207:Cytokines / Q000276:immunology*; D005260:Female; D006801:Humans; D007159:Immunologic Tests / Q000379:methods; D007223:Infant; D008297:Male; D010865:Pilot Projects; D012372:ROC Curve; D014376:Tuberculosis / Q000276:immunology*
chemical_list: D000942:Antigens, Bacterial; D016207:Cytokines
keywords: Children; Cytokines; IL-13; IL-2; IL-5; IP-10; Tuberculosis