title: The crude extract of Corni Fructus inhibits the migration and invasion of U-2 OS human osteosarcoma cells through the inhibition of matrix metalloproteinase-2/-9 by MAPK signaling.
abstract: Osteosarcoma is the most common primary malignancy of the bone cancers. In the Chinese population, the crude extract of Corni Fructus (CECF) has been used as Traditional Chinese medicine to treat several different diseases for hundreds of years. In the present study, effects of CECF on inhibition of migration and invasion in U-2 OS human osteosarcoma cells were examined. CECF significantly inhibited migration and invasion of U-2 OS human osteosarcoma cells. We also found that CECF inhibited activities of matrix metalloproteinases-2 (MMP-2) and matrix metalloproteinases-9 (MMP-9). CECF decreased protein levels of FAK, PKC, SOS1, MKK7, MEKK3, GRB2, NF-κB p65, COX-2, HIF-1α, PI3K, Rho A, ROCK-1, IRE-1α, p-JNK1/2, p-ERK1/2, p-p38, Ras, p-PERK, MMP-2, MMP-9, and VEGF in U-2 OS cells. Results of this study indicate that CECF may have potential as a novel anticancer agent for the treatment of osteosarcoma by inhibiting migration and invasion of cancer cells.
authors: Graduate Institute of Chinese Medicine, China Medical University, Taichung, 404, Taiwan.
authors: Ching-Lung
authors: CL
authors: Liao
authors: Ju-Hwa
authors: JH
authors: Lin
authors: Jin-Cherng
authors: JC
authors: Lien
authors: Shu-Chun
authors: SC
authors: Hsu
authors: Fu-Shin
authors: FS
authors: Chueh
authors: Chien-Chih
authors: CC
authors: Yu
authors: Ping-Ping
authors: PP
authors: Wu
authors: Yi-Ping
authors: YP
authors: Huang
authors: Jaung-Geng
authors: JG
authors: Lin
authors: Jing-Gung
authors: JG
authors: Chung
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000302:isolation & purification / Q000494:pharmacology*; D001859:Bone Neoplasms / Q000473:pathology; D018929:Cell Culture Techniques; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D029768:Cornus / Q000737:chemistry*; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D020935:MAP Kinase Signaling System / Q000187:drug effects*; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism*; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism*; D061965:Matrix Metalloproteinase Inhibitors / Q000302:isolation & purification / Q000494:pharmacology*; D016328:NF-kappa B / Q000378:metabolism; D009361:Neoplasm Invasiveness; D012516:Osteosarcoma / Q000473:pathology
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D004365:Drugs, Chinese Herbal; D061965:Matrix Metalloproteinase Inhibitors; D016328:NF-kappa B; C522361:MMP2 protein, human; D020778:Matrix Metalloproteinase 2; C579270:MMP9 protein, human; D020780:Matrix Metalloproteinase 9
keywords: U-2 OS human osteosarcoma cells; crude extract of Corni Fructus (CECF); invasion; migration