title: Cell migration or cytokinesis and proliferation?--revisiting the "go or grow" hypothesis in cancer cells in vitro.
abstract: The mortality of patients with solid tumors is mostly due to metastasis that relies on the interplay between migration and proliferation. The "go or grow" hypothesis postulates that migration and proliferation spatiotemporally excludes each other. We evaluated this hypothesis on 35 cell lines (12 mesothelioma, 13 melanoma and 10 lung cancer) on both the individual cell and population levels. Following three-day-long videomicroscopy, migration, proliferation and cytokinesis-length were quantified. We found a significantly higher migration in mesothelioma cells compared to melanoma and lung cancer while tumor types did not differ in mean proliferation or duration of cytokinesis. Strikingly, we found in melanoma and lung cancer a significant positive correlation between mean proliferation and migration. Furthermore, non-dividing melanoma and lung cancer cells displayed slower migration. In contrast, in mesothelioma there were no such correlations. Interestingly, negative correlation was found between cytokinesis-length and migration in melanoma. FAK activation was higher in melanoma cells with high motility. We demonstrate that the cancer cells studied do not defer proliferation for migration. Of note, tumor cells from various organ systems may differently regulate migration and proliferation. Furthermore, our data is in line with the observation of pathologists that highly proliferative tumors are often highly invasive.
authors: 2nd Department of Pathology, Semmelweis University, Budapest, Hungary.
authors: Tamás
authors: T
authors: Garay
authors: Éva
authors: É
authors: Juhász
authors: Eszter
authors: E
authors: Molnár
authors: Maria
authors: M
authors: Eisenbauer
authors: András
authors: A
authors: Czirók
authors: Barbara
authors: B
authors: Dekan
authors: Viktória
authors: V
authors: László
authors: Mir Alireza
authors: MA
authors: Hoda
authors: Balázs
authors: B
authors: Döme
authors: József
authors: J
authors: Tímár
authors: Walter
authors: W
authors: Klepetko
authors: Walter
authors: W
authors: Berger
authors: Balázs
authors: B
authors: Hegedűs
mesh_terms: D002465:Cell Movement; D049109:Cell Proliferation; D048749:Cytokinesis; D006801:Humans; D008954:Models, Biological; D009369:Neoplasms / Q000473:pathology*; D014407:Tumor Cells, Cultured
keywords: Cytokinesis; Lung cancer; Melanoma; Mesothelioma; Migration; Proliferation