title: CRISPR/Cas9-targeted mutagenesis in Caenorhabditis elegans.
abstract: The generation of genetic mutants in Caenorhabditis elegans has long relied on the selection of mutations in large-scale screens. Directed mutagenesis of specific loci in the genome would greatly speed up analysis of gene function. Here, we adapt the CRISPR/Cas9 system to generate mutations at specific sites in the C. elegans genome.
authors: Department of Biology, Utrecht University, 3584 CH, Utrecht, The Netherlands.
authors: Selma
authors: S
authors: Waaijers
authors: Vincent
authors: V
authors: Portegijs
authors: Jana
authors: J
authors: Kerver
authors: Bennie B L G
authors: BB
authors: Lemmens
authors: Marcel
authors: M
authors: Tijsterman
authors: Sander
authors: S
authors: van den Heuvel
authors: Mike
authors: M
authors: Boxem
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D001483:Base Sequence; D064113:CRISPR-Cas Systems; D017173:Caenorhabditis elegans / Q000235:genetics*; D018509:DNA, Helminth / Q000235:genetics; D018390:Gene Targeting; D049751:Genome, Helminth; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed
chemical_list: D018509:DNA, Helminth
keywords: CRISPR; Caenorhabditis elegans; Cas9; double-strand break; genome engineering
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