title: The effects of antihypertensive drugs on bone mineral density in ovariectomized mice.
abstract: The effects of several antihypertensive drugs on bone mineral density (BMD) and micro-architectural changes in ovariectomized (OVX) mice were investigated. Eight-week-old female C57/BL6 mice were used for this study. Three days after ovariectomy, mice were treated intraperitoneally with nifedipine (15 mg/kg), telmisartan (5 mg/kg), enalapril (20 mg/kg), propranolol (1 mg/kg) or hydrochlorothiazide (12.5 mg/kg) for 35 consecutive days. Uterine atrophy of all mice was confirmed to evaluate estrogen deficiency state. BMD and micro-architectural analyses were performed on tibial proximal ends by micro-computed tomography (micro-CT). When OVX mice with uterine atrophy were compared with mice without atrophy, BMD decreased (P < 0.001). There were significant differences in BMD loss between different antihypertensive drugs (P = 0.005). Enalapril and propranolol increased BMD loss in mice with atrophied uteri compared with control mice. By contrast, thiazide increased BMD in mice with uterine atrophy compared with vehicle-treated mice (P = 0.048). Thiazide (P = 0.032) and telmisartan (P = 0.051) reduced bone loss and bone fraction in mice with uterine atrophy compared with the control. Thiazide affects BMD in OVX mice positively. The reduction in bone loss by thiazide and telmisartan suggest that these drugs may benefit menopausal women with hypertension and osteoporosis.
authors: Division of Rheumatology, Department of Internal Medicine, The Catholic University of Korea, Seoul, Korea.
authors: Kwi Young
authors: KY
authors: Kang
authors: Yoongoo
authors: Y
authors: Kang
authors: Mirinae
authors: M
authors: Kim
authors: Youngkyun
authors: Y
authors: Kim
authors: Hyoju
authors: H
authors: Yi
authors: Juryun
authors: J
authors: Kim
authors: Hae-Rin
authors: HR
authors: Jung
authors: Sung-Hwan
authors: SH
authors: Park
authors: Ho-Youn
authors: HY
authors: Kim
authors: Ji Hyeon
authors: JH
authors: Ju
authors: Yeon Sik
authors: YS
authors: Hong
mesh_terms: D000818:Animals; D000959:Antihypertensive Agents / Q000494:pharmacology*; D001284:Atrophy; D001562:Benzimidazoles / Q000494:pharmacology; D001565:Benzoates / Q000494:pharmacology; D015519:Bone Density / Q000187:drug effects*; D004656:Enalapril / Q000494:pharmacology; D005260:Female; D051379:Mice; D008810:Mice, Inbred C57BL; D010052:Ovariectomy; D011433:Propranolol / Q000494:pharmacology; D000077333:Telmisartan; D049971:Thiazides / Q000494:pharmacology; D013977:Tibia / Q000000981:diagnostic imaging; D014057:Tomography, X-Ray Computed; D014599:Uterus / Q000033:anatomy & histology / Q000473:pathology
chemical_list: D000959:Antihypertensive Agents; D001562:Benzimidazoles; D001565:Benzoates; D049971:Thiazides; D004656:Enalapril; D011433:Propranolol; D000077333:Telmisartan
keywords: Antihypertensive Agents; Osteoporosis; Ovariectomized Mice; Telmisartan; Thiazides
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