title: Preventive effect of Metformin against N-nitrosodiethylamine-initiated hepatocellular carcinoma in rats.
abstract: Effect of Metformin in chemically induced hepatocarcinogenesis was assessed in Wistar rats. Intraperitoneal administration of chemical carcinogen diethyl nitrosamine (DENA) (200 mg/kg) in single dose elevated the levels of serum glutamate oxaloacetate transaminase (SGOT), serum glutamate pyruvate transaminase (SGPT), alkaline phosphatase (ALP), total cholesterol (TC), triglycerides (TG) and reduced high density lipoproteins (HDL), total proteins (TPR) and blood glucose level in tested animals. Histopathological examinations of the liver tissue showed marked carcinogenicity of the chemical carcinogen. Food and water intake, animal weights and serum albumin (ALB) were also assessed. The animals exposed to DENA showed a significant decrease in the body weights and, there were no significant alterations found in the total bilirubin (TBR) levels and gamma-glutamyltranspeptidase (GGTP), whereas the decreased levels of serum ALB were maintained by Metformin treatment. The elevated levels of serum SGOT, SGPT, ALP, AFP, TC and TG were restored by administration of Metformin in reduced dose (125 mg/kg) daily for 16 weeks p.o. Physiological and biochemical analysis showed the beneficial effects of Metformin in the animals exposed to DENA.
authors: Siddhartha Institute of Pharmacy, Dehra Dun, Uttarakhand, India.
authors: Muhammad
authors: M
authors: Afzal
authors: Imran
authors: I
authors: Kazmi
authors: Gaurav
authors: G
authors: Gupta
authors: Mahfoozur
authors: M
authors: Rahman
authors: Vishwadeepak
authors: V
authors: Kimothi
authors: Firoz
authors: F
authors: Anwar
keywords: Carcinogen; DENA; Hepatocellular carcinogenesis; Histology; Metformin
references: J Clin Endocrinol Metab. 2011 Aug;96(8):2601-8
references: 21752887
references: Toxicology. 2005 May 15;210(1):1-8
references: 15804453
references: Cancer Immunol Immunother. 2011 Sep;60(9):1221-5
references: 21681370
references: Int J Cancer. 1993 Jan 2;53(1):51-5
references: 8380058
references: Chem Biol Interact. 2009 Sep 14;181(1):15-9
references: 19426721
references: Med Oncol. 2012 Jun;29(2):1314-27
references: 21301998
references: Prostaglandins Leukot Essent Fatty Acids. 1998 Jan;58(1):39-54
references: 9482165
references: Breast. 2011 Oct;20 Suppl 3:S31-5
references: 22015290
references: Carcinogenesis. 1999 Dec;20(12):2209-18
references: 10590211
references: Future Oncol. 2011 Jun;7(6):727-36
references: 21675836
references: Pharmacotherapy. 1996 May-Jun;16(3):327-51
references: 8726592
references: Eur J Biochem. 2004 Dec;271(23-24):4745-52
references: 15606761
references: Prostaglandins Leukot Essent Fatty Acids. 2009 Nov-Dec;81(5-6):391-9
references: 19782547
references: Ann Oncol. 2006 Jul;17(7):1083-9
references: 16600982
references: Nanomedicine (Lond). 2012 Apr;7(4):493-506
references: 21995500
references: J Clin Endocrinol Metab. 2012 Apr;97(4):E510-20
references: 22278418
references: N Engl J Med. 1999 Mar 11;340(10):745-50
references: 10072408
references: N Engl J Med. 1995 May 11;332(19):1256-61
references: 7708069
references: Cell Biochem Funct. 2003 Jun;21(2):147-53
references: 12736904
references: Hepatogastroenterology. 2011 Jul-Aug;58(109):1334-42
references: 21937405
references: Lipids Health Dis. 2006 Mar 03;5:4
references: 16515689
references: Biomed Pharmacother. 2002 Jul;56(5):215-22
references: 12199620
references: Liver Int. 2010 May;30(5):750-8
references: 20331505
references: Oncotarget. 2011 Dec;2(12):896-917
references: 22203527
references: Am J Health Syst Pharm. 1997 Apr 15;54(8):893-903
references: 9114921
references: Cleve Clin J Med. 2009 May;76 Suppl 3:S6-9
references: 19465708
references: Prostaglandins Leukot Essent Fatty Acids. 1996 Oct;55(4):257-60
references: 8951994
references: Cancer Res. 1991 Mar 1;51(5):1391-7
references: 1671757
references: Dig Dis Sci. 2011 Oct;56(10):3078-85
references: 21465143
references: J Trace Elem Med Biol. 2010 Apr;24(2):119-23
references: 20413070
references: World J Gastroenterol. 2008 Dec 21;14(47):7192-8
references: 19084933
references: Mol Med Rep. 2012 Apr;5(4):1068-74
references: 22246099
references: Int J Cancer. 2001 Oct 15;94(2):153-6
references: 11668491
references: Lancet. 2003 Dec 6;362(9399):1907-17
references: 14667750
references: Free Radic Biol Med. 1999 May;26(9-10):1314-20
references: 10381205