title: Molecular identification and characterization of the Orco orthologue of Spodoptera litura.
abstract: A highly conserved and broadly expressed receptor protein Orco (olfactory coreceptor) is crucial for insect olfaction, and an orthologue of Orco has been identified in several insect species. Here we report the identification and characterization of Orco from Spodoptera litura. The protein displays high primary amino acid sequence conservation with other previously identified Orco orthologues. Bioinformatic analysis revealed that it has common features with other members of the Orco subfamily: seven-transmembrane domains with intracellular N-terminus and extracellular C-terminus. The transcript was detected in abundance in the chemosensory organs of the antennae of both male and female adults by real-time polymerase chain reaction analysis, and was localized at the bases of all categories of olfactory sensilla through in situ hybridization.
authors: Institute of Health & Environmental Ecology, Wenzhou Medical College, Wenzhou, Zhejiang 325035, China.
authors: Zhong-Nan
authors: ZN
authors: Wu
authors: Xi
authors: X
authors: Chen
authors: Yong-Jun
authors: YJ
authors: Du
authors: Jing-Jiang
authors: JJ
authors: Zhou
authors: Qi-Chuan
authors: QC
authors: ZhuGe
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D005260:Female; D018507:Gene Expression Regulation, Developmental; D008297:Male; D008969:Molecular Sequence Data; D018035:Receptors, Odorant / Q000737:chemistry / Q000235:genetics*; D058618:Sensilla / Q000648:ultrastructure; D012689:Sequence Homology, Nucleic Acid; D018411:Spodoptera / Q000235:genetics* / Q000254:growth & development / Q000648:ultrastructure
chemical_list: D017931:DNA Primers; D018076:DNA, Complementary; D018035:Receptors, Odorant
keywords: Orco orthologue; Spodoptera litura; olfaction; olfactory receptor