title: A chemiluminescence method to detect malondialdehyde in plasma and urine.
abstract: A highly sensitive and convenient method to detect malondialdehyde (MDA) in specimens of plasma and urine was developed using high-performance liquid chromatography combining chemiluminescence detection. MDA was separated through a reverse-phase C18 column at a flow rate of 0.8 ml min(-1). It was then detected by a chemiluminescence detector. Variables that affected chemiluminescence reaction, including mobile phase, flow rate, chemiluminescence reagent, parameters of the photomultiplier tube, and temperature, were studied. This assay was linear from 0.50 to 50 μmol L(-1), with limits of detection and quantification of 0.08 and 0.30 μmol L(-1), respectively. The recoveries were in the range of 92.2 to 98.5%, and the intra- and interday reproducibilities were obtained with relative standard deviations of less than 4% and less than 6%, respectively. MDA in plasma and urine was stable for 12 h at 4°C. MDA levels were 2.74, 3.19, and 3.20 μmol L(-1) for plasma and 1.25, 2.04, and 1.58 μmol L(-1) for urine, respectively. The proposed method is simple, rapid, and sensitive with low cost. Taken together, this method can be suitable for detecting MDA in tissue and feed specimens.
authors: School of Pharmacy, Health Science Center, Xi'an Jiaotong University, Xi'an, 710061 Shaanxi, People's Republic of China; Institute of Analytical Science, Xi'an Jiaotong University, Xi'an, 710061 Shaanxi, People's Republic of China.
authors: Guangbin
authors: G
authors: Zhang
authors: Yuhai
authors: Y
authors: Tang
authors: Xiaoyu
authors: X
authors: Shi
authors: Ruixia
authors: R
authors: Gao
authors: Yang
authors: Y
authors: Sun
authors: Wei
authors: W
authors: Du
authors: Qiang
authors: Q
authors: Fu
mesh_terms: D001798:Blood Proteins / Q000737:chemistry; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D057230:Limit of Detection; D008163:Luminescent Measurements; D008315:Malondialdehyde / Q000097:blood / Q000652:urine; D011489:Protein Denaturation; D012016:Reference Values; D015203:Reproducibility of Results; D013696:Temperature
chemical_list: D001798:Blood Proteins; D008315:Malondialdehyde
keywords: High-performance liquid chromatography–chemiluminescence; Malondialdehyde; Plasma; Urine