title: Ultrasensitive detection of small molecule-protein interaction via terminal protection of small molecule linked DNA and Exo III-aided DNA recycling amplification.
abstract: Terminal protection of small molecule linked DNA assays amplified by Exo III-aided DNA recycling is developed for the detection of streptavidin-biotin interaction, with a detection limit of 0.8 fM streptavidin in the "turn-on" state, indicating that the proposed method is an ultrasensitive platform for the detection of small molecule-protein interactions.
authors: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, China. xhu@whu.edu.cn zhkhe@whu.edu.cn.
authors: Guohua
authors: G
authors: Zhou
authors: Xian
authors: X
authors: Zhang
authors: Xinghu
authors: X
authors: Ji
authors: Zhike
authors: Z
authors: He
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D001710:Biotin / Q000378:metabolism*; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D005090:Exodeoxyribonucleases / Q000737:chemistry / Q000378:metabolism*; D057230:Limit of Detection; D021141:Nucleic Acid Amplification Techniques; D011485:Protein Binding; D019809:Streptavidin / Q000032:analysis / Q000378:metabolism*
chemical_list: D001710:Biotin; D004247:DNA; D019809:Streptavidin; D005090:Exodeoxyribonucleases; C020133:exodeoxyribonuclease III