title: Biological abatement of cellulase inhibitors.
abstract: Removal of enzyme inhibitors released during lignocellulose pretreatment is essential for economically feasible biofuel production. We tested bio-abatement to mitigate enzyme inhibitor effects observed in corn stover liquors after pretreatment with either dilute acid or liquid hot water at 10% (w/v) solids. Bio-abatement of liquors was followed by enzymatic hydrolysis of cellulose. To distinguish between inhibitor effects on enzymes and recalcitrance of the substrate, pretreated corn stover solids were removed and replaced with 1% (w/v) Solka Floc. Cellulose conversion in the presence of bio-abated liquors from dilute acid pretreatment was 8.6% (0.1x enzyme) and 16% (1x enzyme) higher than control (non-abated) samples. In the presence of bio-abated liquor from liquid hot water pretreated corn stover, 10% (0.1x enzyme) and 13% (1x enzyme) higher cellulose conversion was obtained compared to control. Bio-abatement yielded improved enzyme hydrolysis in the same range as that obtained using a chemical (overliming) method for mitigating inhibitors.
authors: Laboratory of Renewable Resources Engineering, Purdue University, West Lafayette, IN 47907-2032, United States; Department of Life Science and Technology, Harbin Institute of Technology, Harbin 150001, China.
authors: Guangli
authors: G
authors: Cao
authors: Laboratory of Renewable Resources Engineering, Purdue University, West Lafayette, IN 47907-2032, United States.
authors: Eduardo
authors: E
authors: Ximenes
authors: Bioenergy Research Unit, National Center for Agricultural Utilization Research, Agricultural Research Service, USDA, 1815 N. University Street, Peoria, IL 61604, United States.
authors: Nancy N
authors: NN
authors: Nichols
authors: Laboratory of Renewable Resources Engineering, Purdue University, West Lafayette, IN 47907-2032, United States.
authors: Leyu
authors: L
authors: Zhang
authors: Laboratory of Renewable Resources Engineering, Purdue University, West Lafayette, IN 47907-2032, United States. Electronic address: ladisch@purdue.edu.
authors: Michael
authors: M
authors: Ladisch
mesh_terms: D000143:Acids / Q000737:chemistry; D001673:Biodegradation, Environmental; D056804:Biofuels; D018533:Biomass; D001709:Biotechnology; D002480:Cellulase / Q000037:antagonists & inhibitors* / Q000737:chemistry; D002482:Cellulose / Q000737:chemistry*; D018556:Crops, Agricultural; D000431:Ethanol / Q000737:chemistry; D005285:Fermentation; D006868:Hydrolysis; D008031:Lignin / Q000737:chemistry*; D013997:Time Factors; D014867:Water / Q000737:chemistry; D003313:Zea mays
chemical_list: D000143:Acids; D056804:Biofuels; D014867:Water; C036909:lignocellulose; D000431:Ethanol; D002482:Cellulose; D008031:Lignin; D002480:Cellulase
keywords: Bio-abatement; Biomass liquor; Enzymatic hydrolysis; Inhibitors