title: Interaction between gene A-positive Helicobacter pylori and human leukocyte antigen II alleles increase the risk of Graves disease in Chinese Han population: an association study.
abstract: OBJECTIVE
  The current study explored the correlation of Helicobacter pylori and the polymorphisms of human leukocyte antigen II (HLA-II) alleles with Graves disease (GD).
  
  
  METHODS
  A total of 216 patients with GD were recruited. 102 healthy volunteers constituted the control group. Levels of H. pylori immunoglobulin G (IgG) antibodies and H. pylori cytotoxin-associated gene A (CagA) IgG antibodies were detected using enzyme-linked immunosorbent assays. Molecular typing of the HLA-II alleles was conducted using polymerase chain reaction with sequence specific primers.
  
  
  RESULTS
  H. pylori, particularly CagA-positive strains, HLA-DQA1 0201, and HLA-DQA1 0501 were associated with GD (P=0.015, OR=1.811; P=0.000, OR=3.085; P=0.000, OR=0.315; and P=0.004, OR=2.844, respectively). Patients with CagA-positive H. pylori and negative HLA-DQA1 0201 or positive HLA-DQA1 0501 were more likely exposed to GD compared with those with only one of these indices.
  
  
  CONCLUSIONS
  CagA-positive H. pylori, negative HLA-DQA1 0201, or positive HLA-DQA1 0501 may increase the risk of GD.
authors: Department of Gastroenterology, Zhongnan Hospital of Wuhan University, Wuhan 430071, China; Department of Endocrinology, Yan'an Affiliated Hospital of Kunming Medical University, Kunming 650051, Yunnan, China.
authors: Yipeng
authors: Y
authors: Wang
authors: Siying
authors: S
authors: Zhu
authors: Yancheng
authors: Y
authors: Xu
authors: Xintao
authors: X
authors: Wang
authors: Youqing
authors: Y
authors: Zhu
mesh_terms: D000328:Adult; D000483:Alleles; D000907:Antibodies, Bacterial / Q000276:immunology; D000942:Antigens, Bacterial / Q000235:genetics* / Q000276:immunology; D001426:Bacterial Proteins / Q000235:genetics* / Q000276:immunology; D016022:Case-Control Studies; D005260:Female; D056726:Genetic Association Studies; D005838:Genotype; D006111:Graves Disease / Q000235:genetics* / Q000276:immunology / Q000382:microbiology*; D059848:HLA-DQ alpha-Chains / Q000235:genetics / Q000276:immunology; D016480:Helicobacter pylori / Q000235:genetics* / Q000276:immunology; D000949:Histocompatibility Antigens Class II / Q000235:genetics* / Q000276:immunology; D006801:Humans; D008297:Male; D012306:Risk; D055815:Young Adult
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D059848:HLA-DQ alpha-Chains; C069050:HLA-DQA1 antigen; D000949:Histocompatibility Antigens Class II; C080866:cagA protein, Helicobacter pylori
keywords: Autoimmune thyroid disease; CI; CagA; EDTA; Ethylene Diamine Tetraacetic Acid; FT3; FT4; GD; Graves disease; H. pylori; HLA; Helicobacter pylori; Human leukocyte antigen; IgG; Immunoglobulin G; OR; PCR; SPSS; Statistical Product and Service Solutions; TPOAb; TRAb; TSH; TgAb; confidence intervals; cytotoxin-associated gene A; dNTP; deoxy-ribonucleoside triphosphate; free thyroxine; free triiodothyronine; human leukocyte antigen; odds ratio; polymerase chain reaction; thyroglobulin antibody; thyroid peroxidase antibody; thyroid stimulating hormone; thyrotrophin receptor antibody