title: Murine marginal zone B cells play a role in Vibrio cholerae LPS antibody responses.
abstract: The emergence of Vibrio cholerae (Vc) lipopolysaccharide (LPS) as a lead protective antigen for a cholera subunit vaccine has increased the interest in what type of B cell is best suited to generate anti-Vc LPS antibodies. A related question is what form of LPS is the most immunogenic. C57Bl/6 (B6) neonatal mice (10 days old) whose marginal zone (MZ) B cell compartment is still maturing and two lines of knockout mice that either lack the signaling mechanism required for the maturation of MZ B cells or that lack a receptor required for MZ B cell retention in the MZ were used to determine the role of MZ B cells in anti-Vc LPS antibody responses. Data support the conclusion that MZ B cells play a significant role in the anti-Vc LPS antibody response. Serum and vibriocidal antibody titers also depend on whether the Vc LPS is purified or bacterial cell-associated.
authors: Department of Microbiology and Immunology, Geisel School of Medicine at Dartmouth, Lebanon, NH, USA.
authors: William F
authors: WF
authors: Wade
authors: R Glenn
authors: RG
authors: King
authors: Cyrille
authors: C
authors: Grandjean
authors: Terri K
authors: TK
authors: Wade
authors: Louis B
authors: LB
authors: Justement
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D000907:Antibodies, Bacterial / Q000097:blood*; D000917:Antibody Formation; D001402:B-Lymphocytes / Q000276:immunology*; D001770:Blood Bactericidal Activity; D008070:Lipopolysaccharides / Q000276:immunology*; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D014734:Vibrio cholerae / Q000276:immunology*
chemical_list: D000907:Antibodies, Bacterial; D008070:Lipopolysaccharides
keywords: LPS; Vibrio cholerae; cholera; vaccine; vibriocidal antibody