title: Fast metabolic response to drug intervention through analysis on a miniaturized, highly integrated molecular imaging system.
abstract: We report on a radiopharmaceutical imaging platform designed to capture the kinetics of cellular responses to drugs.
  
  
  METHODS
  A portable in vitro molecular imaging system comprising a microchip and a β-particle imaging camera permitted routine cell-based radioassays of small numbers of either suspended or adherent cells. We investigated the kinetics of responses of model lymphoma and glioblastoma cancer cell lines to (18)F-FDG uptake after drug exposure. Those responses were correlated with kinetic changes in the cell cycle or with changes in receptor tyrosine kinase signaling.
  
  
  RESULTS
  The platform enabled direct radioassays of multiple cell types and yielded results comparable to those from conventional approaches; however, the platform used smaller sample sizes, permitted a higher level of quantitation, and did not require cell lysis.
  
  
  CONCLUSIONS
  The kinetic analysis enabled by the platform provided a rapid (≈ 1 h) drug screening assay.
authors: NSB Cancer Center, Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, California.
authors: Jun
authors: J
authors: Wang
authors: Kiwook
authors: K
authors: Hwang
authors: Daniel
authors: D
authors: Braas
authors: Alex
authors: A
authors: Dooraghi
authors: David
authors: D
authors: Nathanson
authors: Dean O
authors: DO
authors: Campbell
authors: Yuchao
authors: Y
authors: Gu
authors: Troy
authors: T
authors: Sandberg
authors: Paul
authors: P
authors: Mischel
authors: Caius
authors: C
authors: Radu
authors: Arion F
authors: AF
authors: Chatziioannou
authors: Michael E
authors: ME
authors: Phelps
authors: Heather
authors: H
authors: Christofk
authors: James R
authors: JR
authors: Heath
mesh_terms: D001692:Biological Transport / Q000187:drug effects; D045744:Cell Line, Tumor; D004353:Drug Evaluation, Preclinical / Q000295:instrumentation*; D019788:Fluorodeoxyglucose F18 / Q000378:metabolism; D006019:Glycolysis / Q000187:drug effects; D006801:Humans; D007700:Kinetics; D008904:Miniaturization / Q000295:instrumentation*; D057054:Molecular Imaging / Q000295:instrumentation*; D018511:Systems Integration
chemical_list: D019788:Fluorodeoxyglucose F18
keywords: microfluidics; molecular imaging; radioassay; radiopharmaceuticals
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