title: A validated micellar electrokinetic chromatography method for the quantitation of dexamethasone, ondansetron and aprepitant, antiemetic drugs, in organogel.
abstract: A micellar electrokinetic chromatography (MEKC) method was developed for the determination of three anti-vomiting drugs (aprepitant, dexamethasone and ondansetron) in pharmaceutical formulations. The method was optimized using a central composite design (CCD). Four main factors (borate buffer concentration, pH, methanol content and sodium dodecyl sulfate concentration) were optimized in order to obtain best resolutions and peak efficiencies in a minimum runtime. The separation was performed in a fused-silica capillary. After optimization, the background electrolyte consisted of a borate buffer (62.5mM, pH 8.75) containing sodium dodecyl sulfate (77.5mM) and methanol (3.75%). Under these conditions, a complete separation of each antiemetic drug and its respective internal standards was achieved in 38min. The method was validated with trueness values from 94.9 to 107.2% and precision results (repeatability and intermediate precision) lower than 5.9%. MEKC-UV was the first method allowing the separation of aprepitant, dexamethasone and ondansetron and was suitable for the quantitation of these three antiemetic drugs in organogel formulations. The rapid sample preparation coupled with an automated separation technique make this method convenient for quality control of extemporaneous magistral ready-to-use formulation.
authors: Univ Lille Nord de France, UDSL, EA 4481, UFR Pharmacie, 59000 Lille, France.
authors: Florence
authors: F
authors: Bourdon
authors: Marie
authors: M
authors: Lecoeur
authors: Marion
authors: M
authors: Duhaut
authors: Pascal
authors: P
authors: Odou
authors: Claude
authors: C
authors: Vaccher
authors: Catherine
authors: C
authors: Foulon
mesh_terms: D000932:Antiemetics / Q000032:analysis* / Q000737:chemistry; D000077608:Aprepitant; D002626:Chemistry, Pharmaceutical; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000592:standards*; D003907:Dexamethasone / Q000032:analysis* / Q000737:chemistry; D005782:Gels; D009025:Morpholines / Q000032:analysis* / Q000737:chemistry; D017294:Ondansetron / Q000032:analysis* / Q000737:chemistry
chemical_list: D000932:Antiemetics; D005782:Gels; D009025:Morpholines; D000077608:Aprepitant; D017294:Ondansetron; D003907:Dexamethasone
keywords: Aprepitant; CCD; Central composite design; DOE; Dexamethasone; MEKC; Micellar electrokinetic chromatography; Ondansetron; central composite design; design of experiments; micellar electrokinetic chromatography