title: Acoustic methods for high-throughput protein crystal mounting at next-generation macromolecular crystallographic beamlines.
abstract: To take full advantage of advanced data collection techniques and high beam flux at next-generation macromolecular crystallography beamlines, rapid and reliable methods will be needed to mount and align many samples per second. One approach is to use an acoustic ejector to eject crystal-containing droplets onto a solid X-ray transparent surface, which can then be positioned and rotated for data collection. Proof-of-concept experiments were conducted at the National Synchrotron Light Source on thermolysin crystals acoustically ejected onto a polyimide `conveyor belt'. Small wedges of data were collected on each crystal, and a complete dataset was assembled from a well diffracting subset of these crystals. Future developments and implementation will focus on achieving ejection and translation of single droplets at a rate of over one hundred per second.
authors: Photon Sciences Directorate, Brookhaven National Laboratory, Upton, NY 11973, USA.
authors: Christian G
authors: CG
authors: Roessler
authors: Anthony
authors: A
authors: Kuczewski
authors: Richard
authors: R
authors: Stearns
authors: Richard
authors: R
authors: Ellson
authors: Joseph
authors: J
authors: Olechno
authors: Allen M
authors: AM
authors: Orville
authors: Marc
authors: M
authors: Allaire
authors: Alexei S
authors: AS
authors: Soares
authors: Annie
authors: A
authors: Héroux
mesh_terms: D018360:Crystallography, X-Ray / Q000295:instrumentation* / Q000379:methods*; D003625:Data Collection; D046911:Macromolecular Substances; D011506:Proteins / Q000737:chemistry*; D017356:Synchrotrons
chemical_list: D046911:Macromolecular Substances; D011506:Proteins
keywords: X-ray diffraction; acoustic droplet ejection; conveyor belt; crystal mounting; high throughput; macromolecular crystallography
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