title: Resolution of lysosomes in living cells with a ratiometric molecular pH-meter.
abstract: Intracellular acidic vesicles, constituted mostly by lysosomes, mediated a variety of biological events ranging from endocytosis, apoptosis, to cancer metastasis, etc. A chimeric molecular pH-meter (Lyso-DR), comprised of a dansyl fluorophore and proton activatable rhodamine-lactam, was prepared for ratiometric reporting of intralysosomal acidity. Exclusively confined in lysosomes, Lyso-DR exhibited pH dependent dual fluorescence emission bands which enable resolution of individual lysosomes in terms of acidity and quantitation of the overall intracellular lysosomal acidity, e.g. the lysosomal pH of HeLa cells is around pH 5.0 whereas that of L929 cells is around pH 6.2. Lyso-DR effectively differentiated the lysosomal pH changes of cells undergoing apoptosis vs necrosis, suggesting its utility in investigations on lysosome involved biomedical processes.
authors: Department of Chemical Biology, College of Chemistry and Chemical Engineering, and the Key Laboratory for Chemical Biology of Fujian Province, PR China.
authors: Zhu
authors: Z
authors: Li
authors: Shuqi
authors: S
authors: Wu
authors: Jiahuai
authors: J
authors: Han
authors: Liu
authors: L
authors: Yang
authors: Shoufa
authors: S
authors: Han
mesh_terms: D000818:Animals; D017209:Apoptosis; D002460:Cell Line; D005456:Fluorescent Dyes / Q000737:chemistry; D006367:HeLa Cells; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008247:Lysosomes / Q000737:chemistry*; D051379:Mice; D009336:Necrosis; D012235:Rhodamines / Q000737:chemistry
chemical_list: D005456:Fluorescent Dyes; D012235:Rhodamines
keywords: Apoptosis; Lysosomal acidity; Molecular pH-meter; Ratiometric imaging