title: New trends in LC protein ligand screening.
abstract: The diversity of small molecules available to produce truly innovative drugs associated with the wealth of known biological targets calls for key strategies in protein ligand screening. This review encompasses the recently developed bioaffinity-based strategies. A critical view of the use of zonal, frontal, and nonlinear chromatography with immobilized proteins is given. The association of these elution modes with the ligand fishing method, which uses nanomagnetics particles, is also addressed. A series of applications and how these new screening strategies can be used to determine the function, affinity, and activity parameters of proteins is discussed.
authors: Instituto de Bioquímica Médica, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil; Núcleo de Pesquisas de Produtos Naturais, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.
authors: Marcela C
authors: MC
authors: de Moraes
authors: Kenia L
authors: KL
authors: Vanzolini
authors: Carmen L
authors: CL
authors: Cardoso
authors: Quezia B
authors: QB
authors: Cass
mesh_terms: D002846:Chromatography, Affinity / Q000379:methods*; D006801:Humans; D055767:Immobilized Proteins / Q000737:chemistry; D008024:Ligands; D008280:Magnetics; D053758:Nanoparticles; D011485:Protein Binding; D011506:Proteins / Q000737:chemistry*
chemical_list: D055767:Immobilized Proteins; D008024:Ligands; D011506:Proteins
keywords: Bioaffinity-based strategies; Frontal chromatography; Nonlinear chromatography; Protein ligand screening; Zonal chromatography