title: Maximizing information content of single-molecule FRET experiments: multi-color FRET and FRET combined with force or torque.
abstract: Since its first demonstration about twenty years ago, single-molecule fluorescence resonance energy transfer (FRET) has undergone remarkable technical advances. In this tutorial review, we will discuss two technical advances that increase the information content of the single-molecule FRET measurements: single-molecule multi-color FRET and single-molecule FRET combined with force or torque. Our expectations for future developments will be briefly discussed at the end.
authors: Department of Physics and Astronomy, Seoul National University, Seoul 151-747, Korea. shohng@snu.ac.kr.
authors: Sungchul
authors: S
authors: Hohng
authors: Sanghwa
authors: S
authors: Lee
authors: Jinwoo
authors: J
authors: Lee
authors: Myung Hyun
authors: MH
authors: Jo
mesh_terms: D004867:Equipment Design; D031541:Fluorescence Resonance Energy Transfer / Q000266:history / Q000295:instrumentation* / Q000379:methods*; D049673:History, 20th Century; D049674:History, 21st Century; D008280:Magnetics / Q000295:instrumentation; D018613:Microscopy, Confocal / Q000295:instrumentation / Q000379:methods; D008857:Microscopy, Interference / Q000295:instrumentation / Q000379:methods; D052898:Optical Tweezers