title: Proteomics technology - a powerful tool for the biomedical scientists.
abstract: "Proteomics" refers to the systematic analysis of proteins. It complements other "omics" technologies such as genomics and transcriptomics in elucidating the identity of proteins of an organism, and understanding their functions. Proteomics is used in many areas of research such as discovery of markers for diagnosis and vaccine candidates, understanding pathogenic mechanisms, in the study of expression patterns at different time points and in response to different stimuli, and in elucidating functional protein networks. Proteomics analysis involves sample preparation, protein separation, and protein identification. The 'heart' of current proteomics is mass-spectrometry, with LC-MS/MS and MALDI-TOF/TOF being commonly used equipment. However, the high costs of the equipment, software, databases, and the need for skilled personnel limit the wide utilization of this technology in the less developed countries. Therefore, there need to be sharing of facilities, better networking and collaborations among our scientists and laboratories to take advantage of this powerful technology.
authors: Institute for Research in Molecular Medicine (INFORMM), Universiti Sains Malaysia, 11800 USM, Penang, Malaysia.
authors: Rahmah
authors: R
authors: Noordin
authors: Nurulhasanah
authors: N
authors: Othman
keywords: analysis; mass-spectrometry; proteins; proteomics; technology
references: Infect Disord Drug Targets. 2008 Mar;8(1):31-45
references: 18473905
references: J Cell Biol. 2010 Aug 23;190(4):491-500
references: 20733050
references: Proteomics. 2011 Aug;11(15):3256-68
references: 21726050
references: Clin Microbiol Infect. 2013 Jan;19(1):23-28
references: 23034105