title: Differential utilization of TATA box-binding protein (TBP) and TBP-related factor 1 (TRF1) at different classes of RNA polymerase III promoters.
abstract: In the fruit fly Drosophila melanogaster, RNA polymerase III transcription was found to be dependent not upon the canonical TATA box-binding protein (TBP) but instead upon the TBP-related factor 1 (TRF1) (Takada, S., Lis, J. T., Zhou, S., and Tjian, R. (2000) Cell 101, 459-469). Here we confirm that transcription of fly tRNA genes requires TRF1. However, we unexpectedly find that U6 snRNA gene promoters are occupied primarily by TBP in cells and that knockdown of TBP, but not TRF1, inhibits U6 transcription in cells. Moreover, U6 transcription in vitro effectively utilizes TBP, whereas TBP cannot substitute for TRF1 to promote tRNA transcription in vitro. Thus, in fruit flies, different classes of RNA polymerase III promoters differentially utilize TBP and TRF1 for the initiation of transcription.
authors: Molecular Biology Institute; Departments of Biology.
authors: Neha
authors: N
authors: Verma
authors: Molecular Biology Institute; Chemistry and Biochemistry, San Diego State University, San Diego, California 92182-1030.
authors: Ko-Hsuan
authors: KH
authors: Hung
authors: Molecular Biology Institute; Chemistry and Biochemistry, San Diego State University, San Diego, California 92182-1030.
authors: Jin Joo
authors: JJ
authors: Kang
authors: Molecular Biology Institute; Chemistry and Biochemistry, San Diego State University, San Diego, California 92182-1030.
authors: Nermeen H
authors: NH
authors: Barakat
authors: Molecular Biology Institute; Chemistry and Biochemistry, San Diego State University, San Diego, California 92182-1030. Electronic address: wstumph@mail.sdsu.edu.
authors: William E
authors: WE
authors: Stumph
mesh_terms: D000818:Animals; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D004331:Drosophila melanogaster; D011401:Promoter Regions, Genetic / Q000502:physiology*; D012320:RNA Polymerase III / Q000235:genetics / Q000378:metabolism*; D012342:RNA, Small Nuclear / Q000096:biosynthesis / Q000235:genetics; D012343:RNA, Transfer / Q000096:biosynthesis / Q000235:genetics; D035182:TATA Box Binding Protein-Like Proteins / Q000235:genetics / Q000378:metabolism*; D035181:TATA-Box Binding Protein / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic / Q000502:physiology*
chemical_list: D029721:Drosophila Proteins; D012342:RNA, Small Nuclear; D035182:TATA Box Binding Protein-Like Proteins; D035181:TATA-Box Binding Protein; C441408:TBP-related factor, Drosophila; C405486:U6 small nuclear RNA; D012343:RNA, Transfer; D012320:RNA Polymerase III
keywords: Drosophila; Gene Transcription; General Transcription Factors; RNA Polymerase III; TBP; TRF1; Transcription Initiation Factors; U6 snRNA Transcription; tRNA Transcription
references: Biochem Soc Trans. 2008 Aug;36(Pt 4):590-4
references: 18631122
references: J Biol Chem. 2001 Aug 24;276(34):31786-92
references: 11431466
references: Genes Dev. 2002 Jun 1;16(11):1337-8
references: 12066800
references: Proc Natl Acad Sci U S A. 1991 Aug 15;88(16):7308-12
references: 1871137
references: Mol Cell Biol. 1996 Jan;16(1):1-9
references: 8524284
references: Eur J Biochem. 1997 Aug 15;248(1):231-7
references: 9310383
references: Proc Natl Acad Sci U S A. 1999 Apr 27;96(9):4791-6
references: 10220372
references: Cell. 1997 Oct 3;91(1):71-83
references: 9335336
references: Crit Rev Biochem Mol Biol. 2011 Feb;46(1):11-26
references: 20925482
references: J Biol Chem. 1987 Jan 25;262(3):1187-93
references: 3027083
references: Mol Cell. 2000 Sep;6(3):705-13
references: 11030349
references: EMBO J. 2007 Jan 10;26(1):79-89
references: 17170711
references: Mol Cell Biol. 1993 Sep;13(9):5918-27
references: 8355718
references: Trends Biochem Sci. 2003 Jul;28(7):391-8
references: 12878007
references: J Biol Chem. 1997 Jul 18;272(29):18087-92
references: 9218440
references: Trends Biochem Sci. 1999 Sep;24(9):335-9
references: 10470030
references: Methods Cell Biol. 1994;44:225-35
references: 7707954
references: Cell. 2000 May 26;101(5):459-69
references: 10850489
references: Mol Cell Biol. 1996 Dec;16(12):6909-16
references: 8943346
references: J Mol Biol. 2001 Jun 29;310(1):1-26
references: 11419933
references: FEBS Lett. 2008 Jul 9;582(16):2413-6
references: 18547530
references: Genes Dev. 1993 Jul;7(7B):1291-308
references: 8330735
references: Eur J Biochem. 1993 Feb 15;212(1):1-11
references: 8444147
references: Genes Dev. 2007 Sep 1;21(17):2137-49
references: 17704303
references: Genes Dev. 2007 Nov 15;21(22):2855-60
references: 18006682
references: J Biol Chem. 2001 Jul 20;276(29):26733-6
references: 11390411
references: Genes Dev. 2003 Jun 1;17(11):1309-20
references: 12782648
references: Genes Dev. 1993 Aug;7(8):1535-48
references: 8339931
references: Biochemistry. 2008 Dec 16;47(50):13197-206
references: 19086269
references: Genes Dev. 2002 Oct 15;16(20):2593-620
references: 12381659
references: Proc Natl Acad Sci U S A. 1991 Feb 1;88(3):1024-8
references: 1992453
references: FEBS Lett. 2012 Mar 23;586(6):841-6
references: 22449969
references: Biochim Biophys Acta. 2008 May;1779(5):295-305
references: 18442490
references: Cell. 2000 Sep 29;103(1):5-8
references: 11051541
references: Mol Cell. 2000 Sep;6(3):715-22
references: 11030350
references: Science. 2000 Dec 22;290(5500):2312-5
references: 11125147
references: Genes Dev. 2007 Nov 15;21(22):2936-49
references: 17978101
references: Mol Cell Biol. 2004 Mar;24(5):1897-906
references: 14966271
references: Nature. 1993 Feb 11;361(6412):557-61
references: 8429912
references: Science. 2000 May 5;288(5467):867-70
references: 10797011