title: Proteomic and carbonylation profile analysis of rat skeletal muscles following acute swimming exercise.
abstract: Previous studies by us and other groups characterized protein expression variation following long-term moderate training, whereas the effects of single bursts of exercise are less known. Making use of a proteomic approach, we investigated the effects of acute swimming exercise (ASE) on protein expression and carbonylation patterns in two hind limb muscles: the Extensor Digitorum Longus (EDL) and the Soleus, mostly composed of fast-twitch and slow-twitch fibres, respectively. Carbonylation is one of the most common oxidative modifications of proteins and a marker of oxidative stress. In fact, several studies suggest that physical activity and the consequent increase in oxygen consumption can lead to increase in reactive oxygen and nitrogen species (RONS) production, hence the interest in examining the impact of RONS on skeletal muscle proteins following ASE. Results indicate that protein expression is unaffected by ASE in both muscle types. Unexpectedly, the protein carbonylation level was reduced following ASE. In particular, the analysis found 31 and 5 spots, in Soleus and EDL muscles respectively, whose carbonylation is reduced after ASE. Lipid peroxidation levels in Soleus were markedly reduced as well. Most of the decarbonylated proteins are involved either in the regulation of muscle contractions or in the regulation of energy metabolism. A number of hypotheses may be advanced to account for such results, which will be addressed in future studies.
authors: Department of Experimental and Clinical Biomedical Sciences, University of Florence, Florence, Italy. francesca.magherini@unifi.it
authors: Francesca
authors: F
authors: Magherini
authors: Tania
authors: T
authors: Gamberi
authors: Laura
authors: L
authors: Pietrovito
authors: Tania
authors: T
authors: Fiaschi
authors: Luca
authors: L
authors: Bini
authors: Fabio
authors: F
authors: Esposito
authors: Marina
authors: M
authors: Marini
authors: Provvidenza Maria
authors: PM
authors: Abruzzo
authors: Massimo
authors: M
authors: Gulisano
authors: Alessandra
authors: A
authors: Modesti
mesh_terms: D000818:Animals; D015227:Lipid Peroxidation; D008297:Male; D013058:Mass Spectrometry; D009124:Muscle Proteins / Q000378:metabolism; D018482:Muscle, Skeletal / Q000378:metabolism*; D010805:Physical Conditioning, Animal; D050050:Protein Carbonylation; D020543:Proteome; D040901:Proteomics / Q000379:methods; D051381:Rats; D013550:Swimming
chemical_list: D009124:Muscle Proteins; D020543:Proteome
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