title: Temperature dependence of the interaction of prazosin with lipid Langmuir monolayers.
abstract: The influence of temperature on membrane-prazosin interactions was studied. Prazosin, a quinazoline derivative of 2-furoylpiperazine, is a classic antihypertensive and antiarrhythmic drug. A mixed cholesterol/phospholipid monolayer at the water/air interface was employed as a simplified biomembrane model. Brewster angle microscopy (BAM) was used to visualize the monolayer morphology. It was found that prazosin penetrates Langmuir monolayers and modifies the interactions between membrane components, causing monolayer fluidization. An increase in temperature facilitates penetration of prazosin into the monolayers. Prazosin interacts preferentially with phosphatidylcholine and modifies the morphology of the condensed phase domains of DPPC. In the presence of prazosin, monolayers collapse at lower surface pressures. The difference between the collapse pressures of monolayers on water with and without prazosin increases with temperature.
authors: Department of Physical Chemistry and Electrochemistry, Faculty of Chemistry, Jagiellonian University, Ingardena 3, Kraków 30-060, Poland. Electronic address: gzyl@chemia.uj.edu.pl.
authors: Barbara
authors: B
authors: Gzyl-Malcher
authors: Jadwiga
authors: J
authors: Handzlik
authors: Ewelina
authors: E
authors: Klekowska
mesh_terms: D015060:1,2-Dipalmitoylphosphatidylcholine / Q000737:chemistry; D001812:Blood-Brain Barrier; D002784:Cholesterol / Q000737:chemistry; D004058:Diffusion; D008560:Membrane Fluidity; D008563:Membrane Lipids / Q000737:chemistry*; D008567:Membranes, Artificial; D011224:Prazosin / Q000737:chemistry* / Q000493:pharmacokinetics; D011312:Pressure; D013499:Surface Properties; D013696:Temperature
chemical_list: D008563:Membrane Lipids; D008567:Membranes, Artificial; D015060:1,2-Dipalmitoylphosphatidylcholine; D002784:Cholesterol; D011224:Prazosin
keywords: Cholesterol; Compressibility; Drug; Langmuir monolayer; Phospholipid