title: Cationic perylenediimide as a specific fluorescent binder to mismatch containing DNA.
abstract: Small ligand molecules, which can recognize thermodynamically unstable site within DNA, such as mismatch base pair, abasic site, and single-bulge, have attracted much attention because of their potential diagnostics and biological applications. In this paper, we describe the binding of cationic perylenediimide (cPDI) molecules to thymine-containing mismatch base pair in DNA and the formation of cPDI dimer at the mismatch site. The cPDI dimer exhibits a characteristic excimer emission at 650nm. For T/T mismatch containing DNA, the switching behavior from the PDI dimer (650nm) to the monomer (550nm) emission in specific response to Hg(2+) ion was observed.
authors: Department of Materials Science and Chemistry, Graduate School of Engineering, University of Hyogo, 2167 Shosha, Himeji, Hyogo 671-2280, Japan. takada@eng.u-hyogo.ac.jp
authors: Tadao
authors: T
authors: Takada
authors: Akane
authors: A
authors: Ashida
authors: Mitsunobu
authors: M
authors: Nakamura
authors: Kazushige
authors: K
authors: Yamana
mesh_terms: D020137:Base Pair Mismatch; D002412:Cations / Q000378:metabolism*; D002942:Circular Dichroism; D056831:Coordination Complexes / Q000737:chemistry; D004247:DNA / Q000737:chemistry* / Q000378:metabolism*; D019281:Dimerization; D005453:Fluorescence; D007094:Imides / Q000737:chemistry*; D010569:Perylene / Q000031:analogs & derivatives* / Q000737:chemistry
chemical_list: D002412:Cations; D056831:Coordination Complexes; D007094:Imides; C521332:perylenediimide; D010569:Perylene; D004247:DNA
keywords: DNA; Excimer; Fluorescence; Mismatch base pair; Perylenediimide