title: GABA promotes the competitive selection of dendritic spines by controlling local Ca2+ signaling.
abstract: Activity-dependent competition of synapses plays a key role in neural organization and is often promoted by GABA; however, its cellular bases are poorly understood. Excitatory synapses of cortical pyramidal neurons are formed on small protrusions known as dendritic spines, which exhibit structural plasticity. We used two-color uncaging of glutamate and GABA in rat hippocampal CA1 pyramidal neurons and found that spine shrinkage and elimination were markedly promoted by the activation of GABAA receptors shortly before action potentials. GABAergic inhibition suppressed bulk increases in cytosolic Ca(2+) concentrations, whereas it preserved the Ca(2+) nanodomains generated by NMDA-type receptors, both of which were necessary for spine shrinkage. Unlike spine enlargement, spine shrinkage spread to neighboring spines (<15 μm) and competed with their enlargement, and this process involved the actin-depolymerizing factor ADF/cofilin. Thus, GABAergic inhibition directly suppresses local dendritic Ca(2+) transients and strongly promotes the competitive selection of dendritic spines.
authors: 1] Laboratory of Structural Physiology, Center for Disease Biology and Integrative Medicine, Faculty of Medicine, The University of Tokyo, Bunkyo-ku, Tokyo, Japan. [2] CREST, Japan Science and Technology Agency, Kawaguchi, Saitama, Japan. [3].
authors: Tatsuya
authors: T
authors: Hayama
authors: Jun
authors: J
authors: Noguchi
authors: Satoshi
authors: S
authors: Watanabe
authors: Noriko
authors: N
authors: Takahashi
authors: Akiko
authors: A
authors: Hayashi-Takagi
authors: Graham C R
authors: GC
authors: Ellis-Davies
authors: Masanori
authors: M
authors: Matsuzaki
authors: Haruo
authors: H
authors: Kasai
mesh_terms: D000818:Animals; D020013:Calcium Signaling / Q000502:physiology*; D049229:Dendritic Spines / Q000473:pathology / Q000502:physiology*; D005260:Female; D006624:Hippocampus / Q000378:metabolism / Q000473:pathology; D008297:Male; D009924:Organ Culture Techniques; D051381:Rats; D017207:Rats, Sprague-Dawley; D005680:gamma-Aminobutyric Acid / Q000502:physiology*
chemical_list: D005680:gamma-Aminobutyric Acid
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