title: Comparative analysis of the IGF2 and ZBED6 gene variants and haplotypes reveals significant effect of growth traits in cattle.
abstract: Muscle growth is a complex phenomenon regulated by many factors, whereby net growth results from the combined action of synthesis and turnover. Insulin-like growth factor 2 (IGF2) is a fetal growth and differentiation factor that plays an important role in muscle growth and in myoblast proliferation and differentiation; Zinc finger, BED-type containing 6 (ZBED6) is a novel transcription factor that was identified and shown to act as a repressor of IGF2 transcription in skeletal muscle. In this study, a total of seven single nucleotide polymorphisms (SNPs) were identified, four SNPs in intron 8 of IGF2 and one promoter SNP and two missense mutations in the coding region of ZBED6, two of which were in complete linkage disequilibrium (LD) in the bovine IGF2. The 58 haplotypes were inferred in 1522 individuals representing four purebred cattle breeds from China. The seven SNPs, 79 and 66 combined diplotypes were revealed for association with body mass in Nanyang and Jiaxian cattle populations at five different ages (P < 0.05 or 0.01). The mutant-type variants and haplotype 58 (likely in LD with the beneficial quantitative trait nucleotide allele) was superior for body mass; the heterozygote diplotype of the most common haplotypes 58 was associated with higher body mass compared to either heterozygote or homozygote. The statistical analyses indicated that the mutant-type variants and haplotypes are significantly associated with body mass in study cattle populations at different ages. These data demonstrate that variants and haplotypes are associated with growth traits, and these results may provide important biological insights into the phenotypic differentiation that is associated with adaptation and specialization of cattle breeds.
authors: College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling Shaanxi 712100, China.
authors: Yong-Zhen
authors: YZ
authors: Huang
authors: Zhao-Yang
authors: ZY
authors: Zhan
authors: Yu-Jia
authors: YJ
authors: Sun
authors: Jing
authors: J
authors: Wang
authors: Ming-Xun
authors: MX
authors: Li
authors: Xian-Yong
authors: XY
authors: Lan
authors: Chu-Zhao
authors: CZ
authors: Lei
authors: Chun-Lei
authors: CL
authors: Zhang
authors: Hong
authors: H
authors: Chen
mesh_terms: D000818:Animals; D001835:Body Weight / Q000235:genetics; D002417:Cattle / Q000235:genetics* / Q000254:growth & development*; D002681:China; D014644:Genetic Variation; D006239:Haplotypes / Q000235:genetics*; D006579:Heterozygote; D007335:Insulin-Like Growth Factor II / Q000235:genetics*; D007438:Introns; D015810:Linkage Disequilibrium; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic; D019655:Quantitative Trait, Heritable; D014157:Transcription Factors / Q000235:genetics
chemical_list: D014157:Transcription Factors; D007335:Insulin-Like Growth Factor II