title: Increased hippocampal neurogenesis and accelerated response to antidepressants in mice with specific deletion of CREB in the hippocampus: role of cAMP response-element modulator τ.
abstract: The transcription factor cAMP response element-binding protein (CREB) has been implicated in the pathophysiology of depression as well as in the efficacy of antidepressant treatment. However, altering CREB levels appears to have differing effects on anxiety- and depression-related behaviors, depending on which brain region is examined. Furthermore, many manipulations of CREB lead to corresponding changes in other CREB family proteins, and the impact of these changes has been largely ignored. To further investigate the region-specific importance of CREB in depression-related behavior and antidepressant response, we used Creb(loxP/loxP) mice to localize CREB deletion to the hippocampus. In an assay sensitive to chronic antidepressant response, the novelty-induced hypophagia procedure, hippocampal CREB deletion, did not alter the response to chronic antidepressant treatment. In contrast, mice with hippocampal CREB deletion responded to acute antidepressant treatment in this task, and this accelerated response was accompanied by an increase in hippocampal neurogenesis. Upregulation of the CREB-family protein cAMP response-element modulator (CREM) was observed after CREB deletion. Viral overexpression of the activator isoform of CREM, CREMτ, in the hippocampus also resulted in an accelerated response to antidepressants as well as increased hippocampal neurogenesis. This is the first demonstration of CREMτ within the brain playing a role in behavior and specifically in behavioral outcomes following antidepressant treatment. The current results suggest that activation of CREMτ may provide a means to accelerate the therapeutic efficacy of current antidepressant treatment.
authors: Departments of Pharmacology and Genetics, University of Pennsylvania School of Medicine, Philadelphia, PA, USA.
authors: Brigitta B
authors: BB
authors: Gundersen
authors: Lisa A
authors: LA
authors: Briand
authors: Jennifer L
authors: JL
authors: Onksen
authors: John
authors: J
authors: Lelay
authors: Klaus H
authors: KH
authors: Kaestner
authors: Julie A
authors: JA
authors: Blendy
mesh_terms: D000818:Animals; D000928:Antidepressive Agents / Q000494:pharmacology*; D001973:Bromodeoxyuridine / Q000378:metabolism; D050882:CREB-Binding Protein / Q000172:deficiency* / Q000235:genetics; D051786:Cyclic AMP Response Element Modulator / Q000235:genetics / Q000378:metabolism*; D000229:Dependovirus / Q000235:genetics / Q000378:metabolism; D003891:Desipramine / Q000627:therapeutic use; D000089622:Doublecortin Domain Proteins; D005106:Exploratory Behavior / Q000187:drug effects / Q000502:physiology; D005239:Fear / Q000502:physiology; D005786:Gene Expression Regulation / Q000187:drug effects / Q000235:genetics; D049452:Green Fluorescent Proteins / Q000235:genetics; D006624:Hippocampus / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D018782:Maze Learning / Q000187:drug effects / Q000502:physiology; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D008845:Microinjections; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D009043:Motor Activity / Q000187:drug effects / Q000235:genetics; D055495:Neurogenesis / Q000187:drug effects* / Q000235:genetics; D009479:Neuropeptides / Q000378:metabolism; D013550:Swimming / Q000523:psychology; D013997:Time Factors
chemical_list: D000928:Antidepressive Agents; D000089622:Doublecortin Domain Proteins; D008869:Microtubule-Associated Proteins; D009479:Neuropeptides; D051786:Cyclic AMP Response Element Modulator; D049452:Green Fluorescent Proteins; D050882:CREB-Binding Protein; C494364:Crebbp protein, mouse; D001973:Bromodeoxyuridine; D003891:Desipramine
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