title: Cytogenetic characterization of HB2 epithelial cells from the human breast.
abstract: HB2 is a cell line originated by subcloning of MTSV1-7 mammary luminal epithelial cells isolated from human milk and immortalization via introduction of the gene encoding simian virus 40 (SV40) large T antigen. Despite its wide utilization as non-neoplastic counterpart in assays aimed to elucidating various biochemical and genetical aspects of normal and tumoral breast cells, to our knowledge no literature data have so far appeared concerning the chromosomal characterization of the HB2 cells. Here, we report the cytogenetic characterization of the karyotype of HB2 cells, which puts in evidence the occurrence of changes in chromosomal number and structure and the presence of unidentified chromosomal markers in variable amount. Our results do not detract from the utility of HB2 cells in illustrating fundamental aspects of breast cell biology, but rather interject a note of caution into generalizing results obtained with this cell line to other non-immortalized epithelial cell populations from the human breast. Therefore, this work represents a useful resource for all who want to perform appropriate and focused future studies on this cell line and proposes precise indications for a knowledgeable use of HB2 cells.
authors: Dipartimento di Scienze e Tecnologie Biologiche, Chimiche e Farmaceutiche (STEBICEF), University of Palermo, Edificio 16, Viale delle Scienze, 90128, Palermo, Italy, fabio.caradonna@unipa.it.
authors: Fabio
authors: F
authors: Caradonna
authors: Claudio
authors: C
authors: Luparello
mesh_terms: D001940:Breast / Q000166:cytology*; D002460:Cell Line; D002869:Chromosome Aberrations; D005260:Female; D006801:Humans; D059785:Karyotype; D007621:Karyotyping; D014178:Translocation, Genetic
references: Int J Cancer. 2003 Jan 20;103(3):344-51
references: 12471617
references: Cancer Res. 2005 Oct 15;65(20):9236-44
references: 16230384
references: Mol Cell Biol. 1995 Jan;15(1):1-11
references: 7528324
references: Proc Natl Acad Sci U S A. 1991 May 1;88(9):3520-4
references: 1708884
references: Proc Natl Acad Sci U S A. 2004 Jan 27;101(4):947-52
references: 14732683
references: Histol Histopathol. 2004 Jan;19(1):129-36
references: 14702180
references: Breast Cancer Res. 2005;7(4):171-9
references: 15987472
references: Cancer Lett. 2000 May 1;152(2):201-9
references: 10773413
references: DNA Cell Biol. 2001 May;20(5):265-74
references: 11410163
references: Cell. 2001 Jun 1;105(5):645-55
references: 11389834
references: PLoS One. 2012;7(9):e44636
references: 22957092
references: J Biol Chem. 1995 May 26;270(21):12851-6
references: 7759542
references: Oncogene. 2002 Jan 3;21(1):128-39
references: 11791183
references: J Cell Sci. 1998 Feb;111 ( Pt 4):521-32
references: 9443900
references: Oncogene. 2000 Sep 21;19(40):4592-603
references: 11030148
references: Ann Genet. 1979;22(4):210-3
references: 317782
references: Hum Genet. 1992 Jul;89(5):543-7
references: 1634231
references: Am J Surg Pathol. 2010 Nov;34(11):1723-7
references: 20871391
references: J Cell Biochem. 2011 Aug;112(8):2072-81
references: 21465532
references: J Cell Biochem. 1990 Jan;42(1):13-31
references: 2153691
references: J Cell Sci. 2001 Jun;114(Pt 11):2043-53
references: 11493640
references: Breast Cancer Res Treat. 2012 Jun;133(2):649-57
references: 22042362
references: Cancer Genet Cytogenet. 1997 Jul 15;96(2):106-10
references: 9216715
references: Toxicol In Vitro. 2009 Aug;23(5):943-9
references: 19426795
references: Mutagenesis. 2002 Mar;17(2):99-103
references: 11880537
references: Int J Oncol. 2000 Jun;16(6):1081-90
references: 10811978
references: Biosci Rep. 2011 Oct;31(5):303-7
references: 21517784
references: Hum Genet. 2009 Mar;125(2):189-98
references: 19104841
references: Genes Dev. 1994 Dec 15;8(24):2939-52
references: 8001816
references: J Cell Sci. 1994 Dec;107 ( Pt 12):3557-68
references: 7535787
references: Cancer Genet Cytogenet. 2003 Feb;141(1):56-64
references: 12581899
references: Exp Mol Med. 2007 Aug 31;39(4):450-7
references: 17934332
references: Hepatology. 2000 Nov;32(5):1060-8
references: 11050057
references: Int J Cancer. 2004 Dec 10;112(5):738-46
references: 15386367
references: Genes Chromosomes Cancer. 2007 Aug;46(8):717-23
references: 17444494
references: Mol Cell. 2012 Nov 30;48(4):547-59
references: 23063527
references: Biochimie. 2008 Oct;90(10):1578-90
references: 18625282