title: Detection and isolation of human disseminated tumor cells in the murine bone marrow stem cell niche.
abstract: The presence of disseminated tumor cells (DTCs) in the bone marrow is associated with poor prognosis of cancer patients. However, little is known about the biology of DTCs due to lack of relevant animal models. Here, we describe the methods for detecting and isolating human DTCs from the murine bone marrow niche by PCR using human Alu sequences and by fluorescence-activated cell sorting and immunohistochemistry using anti-HLA antibody. These strategies could be useful for exploring the biology of DTCs.
authors: Department of Periodontics and Oral Medicine, University of Michigan School of Dentistry, Ann Arbor, MI, USA.
authors: Yusuke
authors: Y
authors: Shiozawa
authors: Russell S
authors: RS
authors: Taichman
authors: Evan T
authors: ET
authors: Keller
mesh_terms: D000818:Animals; D001853:Bone Marrow / Q000473:pathology*; D001854:Bone Marrow Cells / Q000378:metabolism; D045744:Cell Line, Tumor; D002469:Cell Separation; D005434:Flow Cytometry; D006801:Humans; D007150:Immunohistochemistry; D051379:Mice; D016513:Mice, SCID; D009368:Neoplasm Transplantation; D009360:Neoplastic Cells, Circulating / Q000378:metabolism*; D055153:Stem Cell Niche