title: Refrex-1, a soluble restriction factor against feline endogenous and exogenous retroviruses.
abstract: The host defense against viral infection is acquired during the coevolution or symbiosis of the host and pathogen. Several cellular factors that restrict retroviral infection have been identified in the hosts. Feline leukemia virus (FeLV) is a gammaretrovirus that is classified into several receptor interference groups, including a novel FeLV-subgroup D (FeLV-D) that we recently identified. FeLV-D is generated by transduction of the env gene of feline endogenous gammaretrovirus of the domestic cat (ERV-DCs) into FeLV. Some ERV-DCs are replication competent viruses which are present and hereditary in cats. We report here the determination of new viral receptor interference groups and the discovery of a soluble antiretroviral factor, termed Refrex-1. Detailed analysis of FeLV-D strains and ERV-DCs showed two receptor interference groups that are distinct from other FeLV subgroups, and Refrex-1 specifically inhibited one of them. Refrex-1 is characterized as a truncated envelope protein of ERV-DC and includes the N-terminal region of surface unit, which is a putative receptor-binding domain, but lacks the transmembrane region. Refrex-1 is efficiently secreted from the cells and appears to cause receptor interference extracellularly. Two variants of Refrex-1 encoded by provirus loci, ERV-DC7 and DC16, are expressed in a broad range of feline tissues. The host retains Refrex-1 as an antiretroviral factor, which may potentially prevent reemergence of the ERVs and the emergence of novel ERV-related viruses in cats. Refrex-1 may have been acquired during endogenization of ERV-DCs and may play an important role in retroviral restriction and antiviral defense in cats.
authors: Laboratory of Molecular Immunology and Infectious Disease, Joint Faculty of Veterinary Medicine.
authors: Jumpei
authors: J
authors: Ito
authors: Shinya
authors: S
authors: Watanabe
authors: Takahiro
authors: T
authors: Hiratsuka
authors: Kyohei
authors: K
authors: Kuse
authors: Yuka
authors: Y
authors: Odahara
authors: Haruyo
authors: H
authors: Ochi
authors: Maki
authors: M
authors: Kawamura
authors: Kazuo
authors: K
authors: Nishigaki
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D044966:Anti-Retroviral Agents / Q000494:pharmacology*; D015153:Blotting, Western; D002415:Cats; D003001:Cloning, Molecular; D005260:Female; D015686:Gene Products, env / Q000494:pharmacology* / Q000502:physiology*; D015752:Genes, env / Q000502:physiology*; D006801:Humans; D047468:Immunoprecipitation; D005256:Leukemia Virus, Feline / Q000472:pathogenicity*; D051379:Mice; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000494:pharmacology* / Q000502:physiology*; D011533:Proviruses / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D012367:RNA, Viral / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D011991:Receptors, Virus / Q000235:genetics / Q000378:metabolism*; D012192:Retroviridae Infections / Q000517:prevention & control* / Q000662:veterinary / Q000821:virology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid; D014412:Tumor Virus Infections / Q000517:prevention & control* / Q000662:veterinary / Q000821:virology; D014762:Viral Interference; D014779:Virus Replication
chemical_list: D044966:Anti-Retroviral Agents; D015686:Gene Products, env; D010446:Peptide Fragments; D012333:RNA, Messenger; D012367:RNA, Viral; D011991:Receptors, Virus
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