title: Ethanolic extract of Aconiti Brachypodi Radix attenuates nociceptive pain probably via inhibition of voltage-dependent Na⁺ channel.
abstract: Aconiti Brachypodi Radix, belonging to the genus of Aconitum (Family Ranunculaceae), are used clinically as anti-rheumatic, anti-inflammatory and anti-nociceptive in traditional medicine of China. However, its mechanism and influence on nociceptive threshold are unknown and need further investigation. The analgesic effects of ethanolic extract of Aconiti Brachypodi Radix (EABR) were thus studied in vivo and in vitro. Three pain models in mice were used to assess the effect of EABR on nociceptive threshold. In vitro study was conducted to clarify the modulation of the extract on the tetrodotoxin-sensitive (TTX-S) sodium currents in rat's dorsal root ganglion (DRG) neurons using whole-cell patch clamp technique. The results showed that EABR (5-20 mg/kg, i.g.) could produce dose-dependent analgesic effect on hot-plate tests as well as writhing response induced by acetic acid. In addition, administration of 2.5-10 mg/kg EABR (i.g.) caused significant decrease in pain responses in the first and second phases of formalin test without altering the PGE₂ production in the hind paw of the mice. Moreover, EABR (10 µg/ml -1 mg/ml) could suppress TTX-S voltage-gated sodium currents in a dose-dependent way, indicating the underlying electrophysiological mechanism of the analgesic effect of the folk plant medicine. Collectively, our results indicated that EABR has analgesic property in three pain models and useful influence on TTX-S sodium currents in DRG neurons, suggesting that the interference with pain messages caused by the modulation of EABR on TTX-S sodium currents in DRG neurones may explain some of its analgesic effect.
authors: College of Pharmacy, South-Central University for Nationalities, Wuhan 430074, PR China.
authors: Wei
authors: W
authors: Ren
authors: Lin
authors: L
authors: Yuan
authors: Jun
authors: J
authors: Li
authors: Xian-Ju
authors: XJ
authors: Huang
authors: Su
authors: S
authors: Chen
authors: Da-Jiang
authors: DJ
authors: Zou
authors: Xiangming
authors: X
authors: Liu
authors: Xin-Zhou
authors: XZ
authors: Yang
mesh_terms: D019342:Acetic Acid; D031841:Aconitum; D000700:Analgesics / Q000494:pharmacology* / Q000627:therapeutic use; D000818:Animals; D015232:Dinoprostone / Q000096:biosynthesis; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D005260:Female; D005557:Formaldehyde; D005727:Ganglia, Spinal / Q000187:drug effects* / Q000502:physiology; D006358:Hot Temperature; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D059226:Nociceptive Pain / Q000209:etiology / Q000378:metabolism* / Q000517:prevention & control; D017288:Pain Threshold / Q000187:drug effects*; D018408:Patch-Clamp Techniques; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology* / Q000627:therapeutic use; D051381:Rats; D017208:Rats, Wistar; D013779:Tetrodotoxin; D061566:Voltage-Gated Sodium Channels / Q000378:metabolism*
chemical_list: D000700:Analgesics; D010936:Plant Extracts; D061566:Voltage-Gated Sodium Channels; D005557:Formaldehyde; D013779:Tetrodotoxin; D015232:Dinoprostone; D019342:Acetic Acid
keywords: Aconiti Brachypodi Radix; analgesic effect; dorsal root ganglion; sodium channel
references: Pain. 1992 Oct;51(1):5-17
references: 1454405
references: J Ethnopharmacol. 2009 Oct 29;126(1):18-30
references: 19651200
references: Pain. 1999 Aug;Suppl 6:S133-S140
references: 10491982
references: J Pharmacobiodyn. 1980 Oct;3(10):514-25
references: 7205533
references: Biochem Biophys Res Commun. 2007 Mar 23;354(4):929-36
references: 17276394
references: J Pharmacol Methods. 1980 Nov;4(3):251-9
references: 7442270
references: Tohoku J Exp Med. 1979 Jun;128(2):175-87
references: 462476
references: Nat Neurosci. 1998 Dec;1(8):653-5
references: 10196578
references: Sci China C Life Sci. 2004 Aug;47(4):340-8
references: 15493475
references: Biol Pharm Bull. 1997 Oct;20(10):1086-91
references: 9353570
references: Prog Neurobiol. 1998 Oct;56(2):211-35
references: 9760702
references: J Ethnopharmacol. 2007 May 30;112(1):211-4
references: 17350195
references: Proc Natl Acad Sci U S A. 2002 Jun 11;99(12):8360-5
references: 12060780
references: J Neurosci. 2002 Dec 1;22(23):10277-90
references: 12451128
references: J Ethnopharmacol. 2002 Jul;81(2):245-50
references: 12065158
references: Neurology. 1996 Jul;47(1):272-6
references: 8710095
references: J Ethnopharmacol. 2010 Oct 28;132(1):200-5
references: 20713147
references: Neurosci Lett. 1997 Oct 10;235(1-2):73-6
references: 9389599
references: Yao Xue Xue Bao. 1983 Jan;18(1):39-44
references: 6858693
references: Reg Anesth Pain Med. 2000 Nov-Dec;25(6):632-8
references: 11097673
references: Pain. 1987 Jul;30(1):103-114
references: 3614974
references: Brain Res. 2000 Jul 14;871(1):98-103
references: 10882788
references: Sci China C Life Sci. 2006 Jun;49(3):274-85
references: 16856497
references: Agents Actions. 1991 Sep;34(1-2):264-9
references: 1665298