title: Fast and high-accuracy localization for three-dimensional single-particle tracking.
abstract: We report a non-iterative localization algorithm that utilizes the scaling of a three-dimensional (3D) image in the axial direction and focuses on evaluating the radial symmetry center of the scaled image to achieve the desired single-particle localization. Using this approach, we analyzed simulated 3D particle images by wide-field microscopy and confocal microscopy respectively, and the 3D trajectory of quantum dots (QDs)-labeled influenza virus in live cells. Both applications indicate that the method can achieve 3D single-particle localization with a sub-pixel precision and sub-millisecond computation time. The precision is almost the same as that of the iterative nonlinear least-squares 3D Gaussian fitting method, but with two orders of magnitude higher computation speed. This approach can reduce considerably the time and costs for processing the large volume data of 3D images for 3D single-particle tracking, which is especially suited for 3D high-precision single-particle tracking, 3D single-molecule imaging and even new microscopy techniques.
authors: Key Laboratory of Analytical Chemistry for Biology and Medicine, College of Chemistry and Molecular Sciences, State Key Laboratory of Virology, and Wuhan Institute of Biotechnology, Wuhan University, Wuhan, 430072, PR China.
authors: Shu-Lin
authors: SL
authors: Liu
authors: Jicun
authors: J
authors: Li
authors: Zhi-Ling
authors: ZL
authors: Zhang
authors: Zhi-Gang
authors: ZG
authors: Wang
authors: Zhi-Quan
authors: ZQ
authors: Tian
authors: Guo-Ping
authors: GP
authors: Wang
authors: Dai-Wen
authors: DW
authors: Pang
mesh_terms: D021621:Imaging, Three-Dimensional / Q000379:methods*; D018613:Microscopy, Confocal / Q000379:methods*; D057054:Molecular Imaging / Q000379:methods*; D009975:Orthomyxoviridae / Q000648:ultrastructure*; D045663:Quantum Dots; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
references: Biophys J. 2008 Dec 15;95(12):6025-43
references: 18835896
references: Biomaterials. 2012 Nov;33(31):7828-33
references: 22854679
references: ACS Nano. 2010 Jun 22;4(6):3015-22
references: 20524630
references: Science. 2008 Feb 8;319(5864):810-3
references: 18174397
references: Nano Lett. 2010 Nov 10;10(11):4732-7
references: 20957984
references: Proc Natl Acad Sci U S A. 2003 Aug 5;100(16):9280-5
references: 12883000
references: ACS Nano. 2012 Nov 27;6(11):10033-41
references: 23102457
references: Proc Natl Acad Sci U S A. 2012 Jan 17;109(3):675-9
references: 22210112
references: Nanoscale. 2011 Nov;3(11):4532-41
references: 21960183
references: Biomaterials. 2011 Oct;32(30):7616-24
references: 21764443
references: ACS Nano. 2012 Jan 24;6(1):141-50
references: 22117089
references: Nano Lett. 2010 Jan;10(1):211-8
references: 20000821
references: Annu Rev Biophys Biomol Struct. 1997;26:373-99
references: 9241424
references: Nat Methods. 2011 May;8(5):417-23
references: 21378978
references: Nat Methods. 2008 Jun;5(6):527-9
references: 18469823
references: ACS Nano. 2012 Oct 23;6(10):8922-32
references: 22957739
references: Chemphyschem. 2009 Oct 5;10(14):2458-64
references: 19760694
references: J Virol. 2011 Jul;85(13):6252-62
references: 21525360
references: J Cell Biol. 2002 Jun 10;157(6):1071-81
references: 12058021
references: Nat Methods. 2011 Jun;8(6):499-508
references: 21552254
references: Biochem Biophys Res Commun. 2007 Feb 9;353(2):231-7
references: 17178111
references: Semin Immunol. 2005 Feb;17(1):3-21
references: 15582485
references: Science. 2005 Jun 3;308(5727):1469-72
references: 15817813
references: Mol Ther. 2011 Jul;19(7):1199-211
references: 21654634
references: Nano Lett. 2013 Mar 13;13(3):980-6
references: 23360249
references: Proc Natl Acad Sci U S A. 2009 Mar 3;106(9):2995-9
references: 19211795
references: ACS Nano. 2011 May 24;5(5):3523-35
references: 21473596
references: Nat Methods. 2012 Jun 10;9(7):724-6
references: 22688415
references: Biophys J. 2004 Jun;86(6):4075-93
references: 15189902
references: Biophys J. 1996 May;70(5):2067-77
references: 9172731