title: Potentiometric glucose biosensor based on core-shell Fe3O4-enzyme-polypyrrole nanoparticles.
abstract: Core-shell Fe3O4-enzyme-polypyrrole (Ppy) nanoparticles with excellent magnetism and conductivity were successfully prepared via the surface modification and enzyme self-encapsulation within Ppy. A novel potentiometric glucose biosensor has been constructed by effectively attaching the proposed Fe3O4-enzyme-Ppy nanoparticles to the surface of the magnetic glassy carbon electrode (MGCE). The optimum biosensing conditions could be provided with polymerization time of pyrrole for 6h and 0.42 mg immobilization amount of Fe3O4-enzyme-Ppy nanoparticles on MGCE. The performance of the developed glucose biosensor was evaluated and the results indicated that a sensitive glucose biosensor could be fabricated. The obtained glucose biosensor presents shorter response time (6 s), wider linear range (0.5 μM to 34 mM), lower limit of detection (LOD, 0.3 μM), high-selectivity monitoring of glucose and good stability (with about 98.1% of the initial response signal retained after 20 days). The analytical application of the glucose biosensor confirms the feasibility of glucose detection in serum sample.
authors: Institute of Materials Science and Engineering, Henan Polytechnic University, Jiaozuo 454000, China; Cultivating Base for Key Laboratory of Environment-friendly Inorganic Materials in University of Henan Province, Henan Polytechnic University, Jiaozuo 454000, China. Electronic address: zhengpengyang@yahoo.com.cn.
authors: Zhengpeng
authors: Z
authors: Yang
authors: Chunjing
authors: C
authors: Zhang
authors: Jianxin
authors: J
authors: Zhang
authors: Wanbei
authors: W
authors: Bai
mesh_terms: D001234:Aspergillus niger / Q000201:enzymology; D015374:Biosensing Techniques / Q000295:instrumentation*; D001786:Blood Glucose / Q000032:analysis*; D004800:Enzymes, Immobilized / Q000737:chemistry*; D052203:Ferrosoferric Oxide / Q000737:chemistry*; D005949:Glucose Oxidase / Q000737:chemistry*; D006801:Humans; D057230:Limit of Detection; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D011108:Polymers / Q000737:chemistry*; D011199:Potentiometry / Q000295:instrumentation; D011758:Pyrroles / Q000737:chemistry*
chemical_list: D001786:Blood Glucose; D004800:Enzymes, Immobilized; D011108:Polymers; D011758:Pyrroles; C067635:polypyrrole; D005949:Glucose Oxidase; D052203:Ferrosoferric Oxide
keywords: Fe(3)O(4)–enzyme–Ppy; Glucose oxidase; Magnetic electrode; Magnetic immobilization of enzyme; Potentiometric biosensing