title: Effect of N-ethylmaleimide, chymotrypsin, and H₂O₂ on the viscoelasticity of human erythrocytes: experimental measurement and theoretical analysis.
abstract: The physiological functions of erythrocytes depend critically on their morphology, deformability, and aggregation capability in response to external physical and chemical stimuli. The dynamic deformability can be described in terms of their viscoelasticity. We applied jumping optical tweezers to trap and stretch individual red blood cells (RBCs) to characterize its viscoelasticity in terms of the Young's modulus and viscosity by analyzing the experimental data of dynamic deformation using a 2-parameter Kelvin solid model. The effects of three chemical agents (N -ethylmaleimide, Chymotrypsin, and Hydrogen peroxide) on RBC's mechanical properties were studied by comparing the Young's modulus and viscosity of RBCs with and without these chemical treatments. Although the effects of each of these chemicals on the molecular structures of RBC may not be exclusive, based on the dominant effect of each chemical, we attempted to dissect the main contributions of different constituents of the RBC membrane to its viscosity and elasticity.
authors: Institute of Biophotonics, National Yang-Ming University, Taipei, Taiwan, ROC.
authors: Yin-Quan
authors: YQ
authors: Chen
authors: Chih-Wei
authors: CW
authors: Chen
authors: Yu-Li
authors: YL
authors: Ni
authors: Yu-Shan
authors: YS
authors: Huang
authors: Orson
authors: O
authors: Lin
authors: Shu
authors: S
authors: Chien
authors: Lanping Amy
authors: LA
authors: Sung
authors: Arthur
authors: A
authors: Chiou
mesh_terms: D002918:Chymotrypsin / Q000494:pharmacology*; D004548:Elasticity / Q000187:drug effects*; D004907:Erythrocyte Deformability / Q000187:drug effects; D004912:Erythrocytes / Q000166:cytology / Q000187:drug effects*; D005033:Ethylmaleimide / Q000494:pharmacology*; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D008954:Models, Biological; D052898:Optical Tweezers; D014783:Viscosity / Q000187:drug effects
chemical_list: D006861:Hydrogen Peroxide; D002918:Chymotrypsin; D005033:Ethylmaleimide
keywords: Chymotrypsin; Erythrocytes; Hydrogen peroxide; Jumping optical tweezers; Kelvin's solid model; N-ethylmaleimide; Viscoelaticity