title: Regulation of ABA dependent wound induced spreading cell death by MYB108.
abstract: Wounding results in the controlled cell death of a few rows of cells adjacent to disrupted cells resulting in physical wound closure, which combined with phenolic compound deposition, prevents water loss and pathogen entry. The control of these processes remains uncharacterized. Cell death in a mutant of Arabidopsis thaliana lacking BOTRYTIS SENSITIVE1/MYB108 (BOS1/MYB108) function was characterized utilizing physiological, cell biological and genetic methods. The bos1 mutant has a wound induced runaway cell death that includes enhanced reactive oxygen species (ROS) production that followed the extent of enhanced cell death. Exogenous abscisic acid (ABA) enhanced wound induced cell death in Col-0 plants and was sufficient to trigger cell death in bos1. Uncontrolled cell death was dependent of the production and perception of ABA. Furthermore, bos1 had altered sensitivity to and accumulation of ABA. Arabidopsis possesses a genetic program controlling the extent of wound inducible cell death. BOS1 acts as a negative regulator of ABA induced cell death, which functions in the control of this wound sealing program. This program is distinct from other known cell death programs in that it is ABA dependent, but independent of salicylate biosynthesis, ethylene, jasmonate, metacaspases and ROS derived from RBOHD and RBOHF.
authors: College of Biological Sciences, China Agricultural University, Beijing, 100193, China.|Division of Plant Biology, Department of Biosciences, University of Helsinki, FI-00014, Helsinki, Finland.
authors: Fuqiang
authors: F
authors: Cui
authors: Division of Plant Biology, Department of Biosciences, University of Helsinki, FI-00014, Helsinki, Finland.|Institute of Technology, University of Tartu, Nooruse 1, Tartu, 50411, Estonia.
authors: Mikael
authors: M
authors: Brosché
authors: Division of Plant Biology, Department of Biosciences, University of Helsinki, FI-00014, Helsinki, Finland.
authors: Nina
authors: N
authors: Sipari
authors: College of Biological Sciences, China Agricultural University, Beijing, 100193, China.
authors: Saijun
authors: S
authors: Tang
authors: Division of Plant Biology, Department of Biosciences, University of Helsinki, FI-00014, Helsinki, Finland.
authors: Kirk
authors: K
authors: Overmyer
mesh_terms: D000040:Abscisic Acid / Q000235:genetics* / Q000378:metabolism / Q000494:pharmacology; D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D016923:Cell Death / Q000235:genetics*; D060467:Disease Resistance / Q000235:genetics*; D018506:Gene Expression Regulation, Plant; D009154:Mutation; D010935:Plant Diseases / Q000235:genetics*; D010937:Plant Growth Regulators / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; C482738:BOTRYTIS SUSCEPTIBLE1 protein, Arabidopsis; D010937:Plant Growth Regulators; D017382:Reactive Oxygen Species; D014157:Transcription Factors; D000040:Abscisic Acid
keywords: Arabidopsis; BOTRYTIS SENSITIVE1; MYB108; abscisic acid (ABA); cell death; lesion mimic; reactive oxygen species (ROS); wounding
references: Adam L, Somerville SC. 1996. Genetic characterization of five powdery mildew disease resistance loci in Arabidopsis thaliana. Plant Journal 9: 341-356.
references: Angelini R, Tisi A, Rea G, Chen MM, Botta M, Federico R, Cona A. 2008. Involvement of polyamine oxidase in wound healing. Plant Physiology 146: 162-177.
references: Arnon DI. 1929. Copper enzymes in isolated chloroplasts. Polyphenoloxidase in Beta vulgaris. Plant Physiology 24: 1-15.
references: Bari R, Jones JDG. 2009. Role of plant hormones in plant defence responses. Plant Molecular Biology 69: 473-488.
references: Bostock RM, Stermer BA. 1989. Perspectives on wound healing in resistance to pathogens. Annual Review of Phytopathology 27: 343-371.
references: Bozhkov PV, Smertenko AP, Zhivotovsky B. 2010. Aspasing out metacaspases and caspases: proteases of many trades. Science Signaling 3: pe48.
references: Cheong YH, Chang H-S, Gupta R, Wang X, Zhu T, Luan S. 2002. Transcriptional profiling reveals novel interactions between wounding, pathogen, abiotic stress, and hormonal responses in Arabidopsis. Plant Physiology 129: 661-677.
references: Coll NS, Vercammen D, Smidler A, Clover C, Van Breusegem F, Dangl JL, Epple P. 2010. Arabidopsis type I metacaspases control cell death. Science 330: 1393-1397.
references: Hildmann T, Ebneth M, Peña-Cortés H, Sánchez-Serrano JJ, Willmitzer L, Prat S. 1992. General roles of abscisic and jasmonic acids in gene activation as a result of mechanical wounding. The Plant Cell 4: 1157-1170.
references: Jabs T, Dietrich RA, Dangl JL. 1996. Initiation of runaway cell death in an Arabidopsis mutant by extracellular superoxide. Science 273: 1853-1856.
references: Jacobs AK, Lipka V, Burton RA, Panstruga R, Strizhov N, Schulze-Lefert P, Fincher GB. 2003. An Arabidopsis callose synthase, GSL5, is required for wound and papillary callose formation. The Plant Cell 15: 2503-2513.
references: Kraepiel Y, Pédron J, Patrit O, Simond-Côte E, Hermand V, Van Gijsegem F. 2011. Analysis of the plant bos1 mutant highlights necrosis as an efficient defence mechanism during D. dadantii/Arabidospis thaliana interaction. PLoS ONE 6: e18991.
references: Lorrain S, Vailleau F, Balagué C, Roby D. 2003. Lesion mimic mutants: keys for deciphering cell death and defense pathways in plants? Trends in Plant Science 8: 263-271.
references: Luo H, Laluk K, Lai Z, Veronese P, Song F, Mengiste T. 2010. The Arabidopsis Botrytis susceptible1 interactor defines a subclass of RING E3 ligases that regulate pathogen and stress responses. Plant Physiology 154: 1766-1782.
references: Mandaokar A, Browse J. 2009. MYB108 acts together with MYB24 to regulate jasmonate-mediated stamen maturation in Arabidopsis. Plant Physiology 149: 851-862.
references: Mengiste T, Chen X, Salmeron J, Dietrich R. 2003. The BOTRYTIS SUSCEPTIBLE1 gene encodes an R2R3MYB transcription factor protein that is required for biotic and abiotic stress responses in Arabidopsis. The Plant Cell 15: 2551-2565.
references: Müller M, Munné-Bosch S. 2011. Rapid and sensitive hormonal profiling of complex plant samples by liquid chromatography coupled to electrospray ionization tandem mass spectrometry. Plant Methods 7: 37.
references: Robert-Seilaniantz A, Grant M, Jones JDG. 2011. Hormone crosstalk in plant disease and defense: more than just JASMONATE-SALICYLATE antagonism. Annual Review of Phytopathology 49: 317-343.
references: Sagi M, Davydov O, Orazova S, Yesbergenova Z, Ophir R, Stratmann JW, Fluhr R. 2004. Plant respiratory burst oxidase homologs impinge on wound responsiveness and development in Lycopersicon esculentum. The Plant Cell 16: 616-628.
references: Torres MA, Dangl JL, Jones JDG. 2002. Arabidopsis gp91phox homologues AtrbohD and AtrbohF are required for accumulation of reactive oxygen intermediates in the plant defense response. Proceedings of the National Academy of Sciences, USA 99: 517-522.
references: Torres MA, Jones JDG, Dangl JL. 2005. Pathogen-induced, NADPH oxidase-derived reactive oxygen intermediates suppress spread of cell death in Arabidopsis thaliana. Nature Genetics 37: 1130-1134.
references: Weiss D, Ori N. 2007. Mechanisms of cross talk between gibberellin and other hormones. Plant Physiology 144: 1240-1246.