title: Differences in vaginal lactobacilli composition of Iranian healthy and bacterial vaginosis infected women: a comparative analysis of their cytotoxic effects with commercial vaginal probiotics.
abstract: BACKGROUND
  Vaginal flora of healthy women is dominated by Lactobacillus species which can prevent bacterial vaginosis.
  
  
  OBJECTIVE
  The current study aimed to determine the differences in vaginal lactobacilli composition of Iranian healthy and bacterial vaginosis (BV) infected women and compared their cytotoxic effects with commercial vaginal probiotics.
  
  
  METHODS
  One hundred and seventy eight vaginal specimens were collected from healthy and BV infected women. Lactobacillus colonies were obtained by culturing on laked blood BHI and MRS medias and genetically defined by 16s rRNA sequencing. Differentiating the specimens to normal, intermediate and BV infected were carried out by Ison and Hey grading protocol. Identification of Lactobacillus strains in vaginal specimens were performed by Multiplex PCR. The inhibitory effects of lactobacilli on Hela (tumoral cervical cells) and HNCF-pi52 (normal cervical cells) were conducted by MTT and trypan blue assays.
  
  
  RESULTS
  L. crispatus, L. gasseri, L. iners, L. jensenii, L. acidophilus and L. rhamnosus were the most frequently occurring species in vagina of healthy Iranian women. L. crispatus and L. jensenni were significantly higher in the normal than in the BV infected groups. Also the cytotoxic effect of L. crispatus on tumoral cervical cells was higher than other lactobacilli including commercial probiotics.
  
  
  CONCLUSIONS
  As L. crispatus and L. jensenni were significantly higher in BV infected women and the cytotoxic effect of L. crispatus on tumoral cervical cells was high, introduction of new probiotics seems necessary.
authors: Medical Genetics Department, Tehran University of Medical Sciences, Tehran, IR Iran.
authors: Elahe
authors: E
authors: Motevaseli
authors: Mahdieh
authors: M
authors: Shirzad
authors: Reza
authors: R
authors: Raoofian
authors: Seyyed-Mohammad
authors: SM
authors: Hasheminasab
authors: Maryam
authors: M
authors: Hatami
authors: Mehdi
authors: M
authors: Dianatpour
authors: Mohammad-Hossein
authors: MH
authors: Modarressi
keywords: Bacterial Vaginosis; Iran; Probiotic; T-Lymphocytes, Cytotoxic; Vagina; Women
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