title: Proteomic identification of target proteins following Drosha knockdown in cervical cancer.
abstract: The nuclear microRNA (miRNA) processing enzyme Drosha is upregulated in cervical cancer, and its overexpression is related to an invasive tumour phenotype. However, the mechanisms that underlie this effect remain poorly understood. The aim of this study was to identify the potential targets of Drosha in cervical cancer. Here, we demonstrated that Drosha knockdown (Drosha-KD) inhibited proliferation, colony formation and the migration of cervical cancer cells in vitro. A global upregulation of proteins in Drosha-KD cells was revealed by two-dimensional gel electrophoresis (2-DE). Eighteen proteins were identified by liquid chromatography and tandem mass spectrometry technology (LC-MS/MS) from 21 selected protein spots that exhibited significant alterations in Drosha-KD cells. The majority of the identified proteins have been previously associated with tumour formation. The downregulation of tubulin 5β in Drosha-KD cervical cancer cells was further confirmed by western blotting. Our results suggest that Drosha affects the biological activity of cervical cancer cells by regulating the expression of numerous tumour-associated proteins.
authors: Department of Obstetrics and Gynecology, Tongji Medical College, Union Hospital, Huazhong University of Science and Technology, Wuhan, Hubei, P.R. China.
authors: Jun
authors: J
authors: Zhou
authors: Jing
authors: J
authors: Cai
authors: Zaiju
authors: Z
authors: Huang
authors: Hui
authors: H
authors: Ding
authors: Junjie
authors: J
authors: Wang
authors: Jinghui
authors: J
authors: Jia
authors: Yong
authors: Y
authors: Zhao
authors: Da
authors: D
authors: Huang
authors: Zehua
authors: Z
authors: Wang
mesh_terms: D045744:Cell Line, Tumor; D002465:Cell Movement; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D055785:Gene Knockdown Techniques; D006801:Humans; D035683:MicroRNAs / Q000235:genetics; D009363:Neoplasm Proteins / Q000096:biosynthesis* / Q000235:genetics; D040901:Proteomics / Q000379:methods*; D043244:Ribonuclease III / Q000096:biosynthesis* / Q000235:genetics; D053719:Tandem Mass Spectrometry; D002583:Uterine Cervical Neoplasms / Q000235:genetics* / Q000473:pathology
chemical_list: D035683:MicroRNAs; D009363:Neoplasm Proteins; C517918:DROSHA protein, human; D043244:Ribonuclease III