title: Interaction of human serum albumin with novel 3,9-disubstituted perylenes.
abstract: Human serum albumin (HSA) has been used as a model for the binding of a number of different ligands, including polyaromatic hydrocarbons, to proteins. In this case we have investigated the interaction of HSA with a novel set of perylene derivatives. Di-substituted perylene analogues have been synthesized as potentially useful organic photovoltaic materials. Their photophysical properties may make them viable for fuel cell applications too. However, these molecules are poorly soluble especially in aqueous solvents. Binding to water-soluble proteins may provide a way to solubilize them. At the same time one can study whether the photophysical processes initiated by the irradiation of a perylene ligand can cause conformational changes to the host protein. With the present study we demonstrated that of the three perylene derivatives investigated only one, the dimethoxy analogue, has a significant affinity for HSA at a binding site near the bottom of the central cleft (in proximity of the Trp214 residue). The small affinity prevents any significant photoinduced changes to occur in the protein.
authors: Department of Physics and Astronomy, University of Texas at San Antonio, San Antonio, TX, USA.
authors: Mohammed J
authors: MJ
authors: Farooqi
authors: Mark A
authors: MA
authors: Penick
authors: George R
authors: GR
authors: Negrete
authors: Lorenzo
authors: L
authors: Brancaleon
mesh_terms: D001665:Binding Sites; D002942:Circular Dichroism; D006801:Humans; D062105:Molecular Docking Simulation; D010569:Perylene / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D011485:Protein Binding; D011487:Protein Conformation; D012709:Serum Albumin / Q000737:chemistry* / Q000378:metabolism*; D013050:Spectrometry, Fluorescence
chemical_list: D012709:Serum Albumin; D010569:Perylene
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