title: Disruption of centrifugal inhibition to olfactory bulb granule cells impairs olfactory discrimination.
abstract: Granule cells (GCs) are the most abundant inhibitory neuronal type in the olfactory bulb and play a critical role in olfactory processing. GCs regulate the activity of principal neurons, the mitral cells, through dendrodendritic synapses, shaping the olfactory bulb output to other brain regions. GC excitability is regulated precisely by intrinsic and extrinsic inputs, and this regulation is fundamental for odor discrimination. Here, we used channelrhodopsin to stimulate GABAergic axons from the basal forebrain selectively and show that this stimulation generates reliable inhibitory responses in GCs. Furthermore, selective in vivo inhibition of GABAergic neurons in the basal forebrain by targeted expression of designer receptors exclusively activated by designer drugs produced a reversible impairment in the discrimination of structurally similar odors, indicating an important role of these inhibitory afferents in olfactory processing.
authors: Department of Biology, University of Maryland, College Park, MD 20742, USA.
authors: Alexia
authors: A
authors: Nunez-Parra
authors: Robert K
authors: RK
authors: Maurer
authors: Krista
authors: K
authors: Krahe
authors: Richard S
authors: RS
authors: Smith
authors: Ricardo C
authors: RC
authors: Araneda
mesh_terms: D000200:Action Potentials / Q000187:drug effects / Q000502:physiology / Q000528:radiation effects; D000818:Animals; D001369:Axons / Q000378:metabolism / Q000502:physiology; D000075402:Channelrhodopsins; D000229:Dependovirus / Q000235:genetics; D004192:Discrimination, Psychological / Q000502:physiology*; D005260:Female; D059330:GABAergic Neurons / Q000378:metabolism / Q000502:physiology; D005968:Glutamate Decarboxylase / Q000235:genetics / Q000378:metabolism; D066298:In Vitro Techniques; D008027:Light; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D018613:Microscopy, Confocal; D009474:Neurons / Q000378:metabolism / Q000502:physiology*; D009812:Odorants; D009830:Olfactory Bulb / Q000166:cytology / Q000502:physiology*; D055696:Olfactory Perception / Q000502:physiology*; D026941:Sodium Channel Blockers / Q000494:pharmacology; D013569:Synapses / Q000187:drug effects / Q000502:physiology / Q000528:radiation effects; D013779:Tetrodotoxin / Q000494:pharmacology
chemical_list: D000075402:Channelrhodopsins; D026941:Sodium Channel Blockers; D013779:Tetrodotoxin; D005968:Glutamate Decarboxylase; C402565:glutamate decarboxylase 1; C401141:glutamate decarboxylase 2
keywords: habituation; uncaging
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