title: ¹H, ¹³C and ¹⁵N NMR assignments of a mutant of UV inducible transcript (S55A-UVI31+) from Chlamydomonas reinhardtii.
abstract: Almost complete sequence specific (1)H, (13)C and (15)N resonance assignments of a mutant of UV inducible transcript (S55A-UVI31+) from Chlamydomonas reinhardtii are reported, as a prelude to its structural and functional characterization. Site-directed mutagenesis of uvi31+ was carried out using complementary mutation harbouring oligonucleotides for S55A mutant. The resulting mutant was sequenced and then S55A mutant of uvi31+ cDNA were expressed in E. coli, and purification were carried out from where the protein was purified to high homogeneity. The point mutation S55A in UVI31+ results in a significant enhancement in its DNA endonuclease activity as compared to its wild type protein.
authors: Department of Chemical Sciences, Tata Institute of Fundamental Research, 1, Homi Bhabha Road, Colaba, Mumbai, 400005, India.
authors: Himanshu
authors: H
authors: Singh
authors: B J
authors: BJ
authors: Rao
authors: Kandala V R
authors: KV
authors: Chary
mesh_terms: D016825:Chlamydomonas reinhardtii / Q000235:genetics*; D016297:Mutagenesis, Site-Directed; D009154:Mutation; D019906:Nuclear Magnetic Resonance, Biomolecular; D010940:Plant Proteins / Q000737:chemistry* / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D014466:Ultraviolet Rays
chemical_list: D010940:Plant Proteins; D012333:RNA, Messenger