title: The immunomodulatory effects of Sutherlandia frutescens extracts in human normal peripheral blood mononuclear cells.
abstract: Sutherlandia frutescens (SF) is one of the medicinal plants used as an immune booster in the treatment of chronic ailments such as HIV/AIDS and cancer. Limited data suggest that its efficacy is based on its regulatory effect on cytokines, the critical components of the immune response. In this study, we investigated the in vitro immunomodulatory effects of SF extracts on normal human peripheral blood mononuclear cells (PBMCs). An ELISA-based assay was used to assess the levels of expression of 12 cytokines in treated cells. An adenosine triphosphate (ATP) assay was used to assess cell viability in relation to cytokine secretion. SF ethanol extracts induced changes in cytokine secretion relative to the dose of the extract. Generally cytokine expression and secretion was low in concentration because were not stimulated with any endotoxin. The high SFE dose (2.5 mg/ml) significantly (p<0.001) decreased some cytokines including TNF-α and IL 1β. Low doses of this extract (0.5 mg/ml) did not change TNF-α and IL 1β secretion from the baseline (untreated cells). Changes in cytokine secretion of SFE treated cells tracked changes in ATP levels (cell viability). The SFW extract-induced changes in cytokine secretion were independent of cell viability. TNF-α was decreased (p<0.001) by the high dose of SFW extract while IL 1β and IFNγ were increased (p<0.01) by the same dose. High doses decreased cell viability which was reflected in cytokine secretion. It is evident, from these results, that SF extracts can modulate cytokine secretion in unstimulated normal PBMCs in vitro. Further studies in animal models are recommended to advance understanding of this immunomodulatory activity.
authors: Mycotoxin Research Unit, School of Medical Sciences, Faculty of Health Sciences, South African Research Chair: Indigenous Health Care Systems, University of KwaZulu-Natal, Durban, South Africa.
authors: Mlungisi
authors: M
authors: Ngcobo
authors: Nceba
authors: N
authors: Gqaleni
authors: Paul K
authors: PK
authors: Chelule
authors: Metse
authors: M
authors: Serumula
authors: Alain
authors: A
authors: Assounga
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D007887:Fabaceae; D006801:Humans; D007155:Immunologic Factors / Q000494:pharmacology*; D007371:Interferon-gamma / Q000378:metabolism*; D053583:Interleukin-1beta / Q000378:metabolism*; D007963:Leukocytes, Mononuclear / Q000187:drug effects* / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
chemical_list: D007155:Immunologic Factors; D053583:Interleukin-1beta; D010936:Plant Extracts; D014409:Tumor Necrosis Factor-alpha; D007371:Interferon-gamma; D000255:Adenosine Triphosphate
keywords: ELISA; Sutherlandia frutescens; cell viability; cytokines; immune response
references: J Ethnopharmacol. 2010 Mar 2;128(1):1-14
references: 20079412
references: J Ethnopharmacol. 2007 Jun 13;112(2):312-8
references: 17433588
references: Cytokine Growth Factor Rev. 2007 Feb-Apr;18(1-2):171-82
references: 17329145
references: Cancer Lett. 2005 Jan 31;218(1):21-31
references: 15639337
references: J Ethnopharmacol. 2004 Nov;95(1):1-5
references: 15374599
references: J Immunoassay Immunochem. 2010;31(2):169-80
references: 20391028
references: J Ethnopharmacol. 2005 Apr 8;98(1-2):163-70
references: 15763378
references: J Ethnopharmacol. 2008 Oct 28;119(3):342-55
references: 18577439
references: Mol Immunol. 2005 Feb;42(2):161-82
references: 15488606
references: Nutr J. 2005 May 31;4:19
references: 15927053
references: J Ethnopharmacol. 2009 Jul 6;124(1):45-60
references: 19527821
references: Cytokine. 2008 Sep;43(3):374-9
references: 18701317
references: J Ethnopharmacol. 2004 Jul;93(1):9-19
references: 15182898
references: PLoS Clin Trials. 2007 Apr 27;2(4):e16
references: 17476314
references: Rev Diabet Stud. 2004 Fall;1(3):145-9
references: 17491678
references: Lancet. 2001 Jun 2;357(9270):1777-89
references: 11403834
references: J Ethnopharmacol. 2008 Oct 28;119(3):620-9
references: 18761068
references: Mol Aspects Med. 2007 Feb;28(1):59-86
references: 17306360
references: J Allergy Clin Immunol. 2003 Feb;111(2 Suppl):S460-75
references: 12592293