title: Non-smoky glycosyltransferase1 prevents the release of smoky aroma from tomato fruit.
abstract: Phenylpropanoid volatiles are responsible for the key tomato fruit (Solanum lycopersicum) aroma attribute termed "smoky." Release of these volatiles from their glycosylated precursors, rather than their biosynthesis, is the major determinant of smoky aroma in cultivated tomato. using a combinatorial omics approach, we identified the non-smoky glycosyltransferase1 (NSGT1) gene. Expression of NSGT1 is induced during fruit ripening, and the encoded enzyme converts the cleavable diglycosides of the smoky-related phenylpropanoid volatiles into noncleavable triglycosides, thereby preventing their deglycosylation and release from tomato fruit upon tissue disruption. In an nsgt1/nsgt1 background, further glycosylation of phenylpropanoid volatile diglycosides does not occur, thereby enabling their cleavage and the release of corresponding volatiles. Using reverse genetics approaches, the NSGT1-mediated glycosylation was shown to be the molecular mechanism underlying the major quantitative trait locus for smoky aroma. Sensory trials with transgenic fruits, in which the inactive nsgt1 was complemented with the functional NSGT1, showed a significant and perceivable reduction in smoky aroma. NSGT1 may be used in a precision breeding strategy toward development of tomato fruits with distinct flavor phenotypes.
authors: Plant Research International, 6700 AA Wageningen, The Netherlands.
authors: Yury M
authors: YM
authors: Tikunov
authors: Jos
authors: J
authors: Molthoff
authors: Ric C H
authors: RC
authors: de Vos
authors: Jules
authors: J
authors: Beekwilder
authors: Adele
authors: A
authors: van Houwelingen
authors: Justin J J
authors: JJ
authors: van der Hooft
authors: Mariska
authors: M
authors: Nijenhuis-de Vries
authors: Caroline W
authors: CW
authors: Labrie
authors: Wouter
authors: W
authors: Verkerke
authors: Henri
authors: H
authors: van de Geest
authors: Marcela
authors: M
authors: Viquez Zamora
authors: Silvia
authors: S
authors: Presa
authors: Jose Luis
authors: JL
authors: Rambla
authors: Antonio
authors: A
authors: Granell
authors: Robert D
authors: RD
authors: Hall
authors: Arnaud G
authors: AG
authors: Bovy
mesh_terms: D002853:Chromatography, Liquid; D020090:Chromosome Segregation / Q000235:genetics; D032461:Chromosomes, Plant / Q000235:genetics; D005054:Eugenol / Q000737:chemistry; D005638:Fruit / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D018506:Gene Expression Regulation, Plant; D005819:Genetic Markers; D018745:Genome, Plant / Q000235:genetics; D006027:Glycosides / Q000737:chemistry / Q000378:metabolism; D006031:Glycosylation; D016695:Glycosyltransferases / Q000378:metabolism*; D006139:Guaiacol / Q000737:chemistry; D006801:Humans; D018551:Solanum lycopersicum / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D013058:Mass Spectrometry; D055442:Metabolome / Q000235:genetics; D008969:Molecular Sequence Data; D009812:Odorants / Q000032:analysis*; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified; D012459:Salicylates / Q000737:chemistry; D014158:Transcription, Genetic
chemical_list: D005819:Genetic Markers; D006027:Glycosides; D010940:Plant Proteins; D012459:Salicylates; D005054:Eugenol; D006139:Guaiacol; D016695:Glycosyltransferases; C033069:methyl salicylate
references: Plant J. 2009 Jun;58(6):961-9
references: 19222805
references: Bioinformatics. 2007 Nov 1;23(21):2947-8
references: 17846036
references: Annu Rev Plant Biol. 2005;56:301-25
references: 15862098
references: Annu Rev Plant Biol. 2006;57:567-97
references: 16669774
references: Curr Opin Plant Biol. 2005 Jun;8(3):254-63
references: 15860422
references: Plant J. 2012 Mar;69(6):1043-51
references: 22103597
references: Nat Prod Rep. 2011 Oct;28(10):1626-95
references: 21845280
references: J Nat Med. 2010 Oct;64(4):500-5
references: 20574729
references: Mol Biol Evol. 2011 Oct;28(10):2731-9
references: 21546353
references: Plant Physiol. 2010 Jan;152(1):55-70
references: 19889876
references: Plant Biotechnol J. 2009 Jan;7(1):106-17
references: 19021875
references: Biosci Biotechnol Biochem. 2006 Nov;70(11):2797-9
references: 17090925
references: Plant Physiol. 2005 Nov;139(3):1125-37
references: 16286451
references: J Agric Food Chem. 2003 Dec 31;51(27):8036-44
references: 14690393
references: Appl Microbiol Biotechnol. 2010 Mar;86(1):109-17
references: 19902208
references: Theor Appl Genet. 2006 Mar;112(5):934-44
references: 16402187
references: Nucleic Acids Res. 1989 Oct 25;17(20):8385
references: 2682529
references: Plant J. 2004 Oct;40(1):88-100
references: 15361143
references: Plant Physiol. 2004 Aug;135(4):1993-2011
references: 15286288
references: J Agric Food Chem. 2007 Dec 26;55(26):10897-901
references: 18052239
references: Lett Appl Microbiol. 2003;36(1):9-14
references: 12485334
references: J Agric Food Chem. 2007 Oct 31;55(22):9170-6
references: 17902628
references: Plant J. 2007 Dec;52(5):865-76
references: 17883372
references: Nutr Rev. 1990 Feb;48(2):87-93; discussion 114-31
references: 2407984
references: Nat Biotechnol. 2007 Aug;25(8):899-901
references: 17592476
references: J Biol Chem. 2005 Jan 14;280(2):899-906
references: 15509561
references: Biotechniques. 1995 Nov;19(5):734-7
references: 8588907
references: Plant Cell Physiol. 2009 Aug;50(8):1401-15
references: 19561332
references: Proc Natl Acad Sci U S A. 2006 Jun 27;103(26):10128-33
references: 16782809
references: Plant Physiol. 2004 Aug;135(4):1893-902
references: 15326281
references: Plant J. 2005 May;42(3):353-63
references: 15842621
references: Curr Biol. 2012 Jun 5;22(11):1035-9
references: 22633806
references: Plant Physiol. 2002 Dec;130(4):2164-76
references: 12481100
references: J Exp Bot. 2009;60(7):2139-54
references: 19346240
references: Nature. 2012 May 30;485(7400):635-41
references: 22660326
references: Nature. 2001 Nov 29;414(6863):562-5
references: 11734859
references: Plant Physiol. 2006 Aug;141(4):1205-18
references: 16896233
references: Plant Physiol. 2000 Mar;122(3):627-33
references: 10712525
references: Evolution. 1985 Jul;39(4):783-791
references: 28561359
references: Chem Senses. 1999 Jun;24(3):327-35
references: 10400451
references: Nat Prod Rep. 2012 Jun;29(6):697-709
references: 22504390
references: Plant J. 2010 Apr 1;62(1):113-23
references: 20070566
references: Nahrung. 1998 Dec;42(6):395-9
references: 9881368