title: Light-addressed electrodeposition of enzyme-entrapped chitosan membranes for multiplexed enzyme-based bioassays using a digital micromirror device.
abstract: This paper describes a light-addressed electrolytic system used to perform an electrodeposition of enzyme-entrapped chitosan membranes for multiplexed enzyme-based bioassays using a digital micromirror device (DMD). In this system, a patterned light illumination is projected onto a photoconductive substrate serving as a photo-cathode to electrolytically produce hydroxide ions, which leads to an increased pH gradient. The high pH generated at the cathode can cause a local gelation of chitosan through sol-gel transition. By controlling the illumination pattern on the DMD, a light-addressed electrodeposition of chitosan membranes with different shapes and sizes, as well as multiplexed micropatterning, was performed. The effect of the illumination time of the light pattern on the dimensional resolution of chitosan membrane formation was examined experimentally. Moreover, multiplexed enzyme-based bioassay of enzyme-entrapped chitosan membranes was also successfully demonstrated through the electrodeposition of the chitosan membranes with various shapes/sizes and entrapping different enzymes. As a model experiment, glucose and ethanol were simultaneously detected in a single detection chamber without cross-talk using shape-coded chitosan membranes entrapped with glucose oxidase (GOX), peroxidase (POD), and Amplex Red (AmR) or alcohol oxidase (AOX), POD, and AmR by using same fluorescence indicator (AmR).
authors: Department of Mechanical and Mechatronic Engineering, National Taiwan Ocean University, Keelung 202-24, Taiwan. shihhao@mail.ntou.edu.tw
authors: Shih-Hao
authors: SH
authors: Huang
authors: Lu-Shiuan
authors: LS
authors: Wei
authors: Hsiao-Tzu
authors: HT
authors: Chu
authors: Yeu-Long
authors: YL
authors: Jiang
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation*; D048271:Chitosan / Q000737:chemistry / Q000528:radiation effects; D004595:Electroplating / Q000379:methods; D004797:Enzyme-Linked Immunosorbent Assay / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007909:Lenses; D008027:Light; D008567:Membranes, Artificial; D008904:Miniaturization; D054802:Molecular Imprinting / Q000379:methods*; D012815:Signal Processing, Computer-Assisted / Q000295:instrumentation*; D013050:Spectrometry, Fluorescence / Q000295:instrumentation*; D014159:Transducers
chemical_list: D008567:Membranes, Artificial; D048271:Chitosan
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