title: Salinity manipulation as an effective method for enhanced starch production in the marine microalga Tetraselmis subcordiformis.
abstract: Microalgal starch is considered a promising feedstock for bioethanol production. The biomass and starch accumulation in the marine microalga Tetraselmis subcordiformis were characterized under different salinities in response to nitrogen repletion (+N) or depletion (-N) at high irradiance (HI) or low irradiance (LI). Under favorable nutritional conditions (HI+N), biomass accumulation was seldom affected under 20% normal salinity, though starch accumulation were somewhat reduced. Increased salinity impaired overall biomass and starch accumulation, though it led to a temporary starch accumulation at initial cultivation phase. Under nitrogen deprivation, decreased salinity strengthened biomass and starch accumulation regardless of irradiance. The highest starch content of 58.2% dry weight and starch productivity of 0.62 g L(-1) d(-1) were obtained under HI-N with 20% normal salinity. Decreased salinity combined with -N generated moderate stress to facilitate starch accumulation. Salinity manipulation can be effectively applied for enhanced starch production in marine microalgae.
authors: Marine Bioproducts Engineering Group, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China; University of Chinese Academy of Sciences, Beijing 100039, China.
authors: Chang-Hong
authors: CH
authors: Yao
authors: Marine Bioproducts Engineering Group, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.
authors: Jiang-Ning
authors: JN
authors: Ai
authors: Marine Bioproducts Engineering Group, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.
authors: Xu-Peng
authors: XP
authors: Cao
authors: Marine Bioproducts Engineering Group, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China. Electronic address: xuesong@dicp.ac.cn.
authors: Song
authors: S
authors: Xue
mesh_terms: D056804:Biofuels; D018533:Biomass; D002734:Chlorophyll / Q000737:chemistry; D000431:Ethanol / Q000737:chemistry; D008027:Light; D058086:Microalgae / Q000378:metabolism* / Q000528:radiation effects; D009566:Nitrates / Q000737:chemistry; D009584:Nitrogen / Q000737:chemistry; D009995:Osmosis; D010788:Photosynthesis; D054712:Salinity; D012492:Salts / Q000737:chemistry; D013213:Starch / Q000096:biosynthesis*; D013997:Time Factors
chemical_list: D056804:Biofuels; D009566:Nitrates; D012492:Salts; D002734:Chlorophyll; D000431:Ethanol; D013213:Starch; D009584:Nitrogen
keywords: +N; ASW; ASW–N; CEF; Chl; DW; HI; Irradiance; LI; NPQ; Nitrogen deprivation; PS I; PS II; ROS; Salinity; Starch; Tetraselmis subcordiformis; artificial seawater; chlorophyll; cyclic electron flow; dry weight; energy-dependent non-photochemical quenching; high irradiance; low irradiance; nitrogen deprivation; nitrogen repletion; nitrogen-deprived artificial seawater; non-photochemical quenching; photosystem I; photosystem II; qE; reactive oxygen species; −N