title: Microbeam methodologies as powerful tools in manganese hyperaccumulation research: present status and future directions.
abstract: Microbeam studies over the past decade have garnered unique insight into manganese (Mn) homeostasis in plant species that hyperaccumulate this essential mineral micronutrient. Electron- and/or proton-probe methodologies employed to examine tissue elemental distributions have proven highly effective in illuminating excess foliar Mn disposal strategies, some apparently unique to Mn hyperaccumulating plants. When applied to samples prepared with minimal artefacts, these are powerful tools for extracting true 'snapshot' data of living systems. For a range of reasons, Mn hyperaccumulation is particularly suited to in vivo interrogation by this approach. Whilst microbeam investigation of metallophytes is well documented, certain methods originally intended for non-biological samples are now widely applied in biology. This review examines current knowledge about Mn hyperaccumulators with reference to microbeam methodologies, and discusses implications for future research into metal transporters.
authors: Department of Botany, La Trobe University Bundoora, VIC, Australia.
authors: Denise R
authors: DR
authors: Fernando
authors: Alan
authors: A
authors: Marshall
authors: Alan J M
authors: AJ
authors: Baker
authors: Takafumi
authors: T
authors: Mizuno
keywords: Alyxia rubricaulis; Gossia; Maytenus cunninghamii; Mn hyperaccumulator; microbeam analysis
references: New Phytol. 2000 May;146(2):179-181
references: 33862980
references: Plant Cell Environ. 2006 May;29(5):1012-20
references: 17087483
references: Plant Physiol. 1999 Jan;119(1):305-12
references: 9880373
references: Plant J. 2004 Jan;37(2):269-81
references: 14690510
references: New Phytol. 2006;171(4):751-7
references: 16918546
references: Scan Electron Microsc. 1980;(Pt 2):395-408
references: 7423124
references: New Phytol. 2005 Sep;167(3):733-42
references: 16101910
references: New Phytol. 2003 Sep;159(3):691-699
references: 33873594
references: Plant J. 2010 Sep;63(5):870-9
references: 20561256
references: Funct Plant Biol. 2002 Jul;29(7):899-905
references: 32689538
references: J Environ Sci (China). 2006;18(4):746-51
references: 17078555
references: J Environ Qual. 2001 Nov-Dec;30(6):1919-26
references: 11789997
references: Proc Natl Acad Sci U S A. 2000 Apr 25;97(9):4991-6
references: 10781110
references: Plant Cell. 2012 May;24(5):2155-67
references: 22589467
references: Sci Rep. 2012;2:286
references: 22368778
references: New Phytol. 2002 Nov;156(2):195-203
references: 33873285
references: Planta. 2012 Nov;236(5):1459-70
references: 22772585
references: New Phytol. 2009;181(3):637-50
references: 19054339
references: J Microsc. 1998 Jun;190(Pt 3):305-16
references: 9674156
references: Tree Physiol. 2005 Jan;25(1):85-92
references: 15519989
references: Biochem J. 1988 Jun 1;252(2):401-8
references: 3415663
references: Funct Plant Biol. 2008 May;35(3):193-200
references: 32688773
references: Plant Physiol. 1996 Dec;112(4):1715-1722
references: 12226473
references: New Phytol. 2004 Jun;162(3):563-567
references: 33873764
references: New Phytol. 2010 Dec;188(4):1014-27
references: 20819177
references: New Phytol. 2002 Jul;155(1):1-4
references: 33873303
references: Environ Pollut. 2004 Oct;131(3):393-9
references: 15261402
references: Ann Bot. 2009 Apr;103(6):931-9
references: 19211572
references: Plant Cell. 2003 May;15(5):1131-42
references: 12724539
references: Plant Cell. 2010 Mar;22(3):904-17
references: 20228245
references: New Phytol. 1993 Nov;125(3):529-546
references: 33874598
references: J Environ Qual. 2007 Aug 31;36(5):1429-43
references: 17766822
references: Proc Natl Acad Sci U S A. 2007 May 15;104(20):8532-7
references: 17494768
references: Planta. 2006 Dec;225(1):193-202
references: 16821042
references: Plant Physiol. 1998 Oct;118(2):493-504
references: 9765534
references: Scan Electron Microsc. 1980;(Pt 2):335-48
references: 7423122
references: New Phytol. 2003 Dec;160(3):479-488
references: 33873657
references: New Phytol. 2008;177(1):178-185
references: 17986181
references: Planta. 2000 Dec;212(1):75-84
references: 11219586
references: J Exp Bot. 2001 Dec;52(365):2291-300
references: 11709579
references: Plant Physiol Biochem. 2005 Aug;43(8):793-801
references: 16198592
references: Plant J. 2007 Jul;51(2):198-210
references: 17559518
references: Am J Bot. 2013 Apr;100(4):690-700
references: 23507734
references: Nature. 1974 Apr 26;248(5451):807-8
references: 4835556
references: Environ Sci Technol. 2004 Nov 1;38(21):5797-802
references: 15575302