title: Nutritional control of epigenetic processes in yeast and human cells.
abstract: The vitamin folate is required for methionine homeostasis in all organisms. In addition to its role in protein synthesis, methionine is the precursor to S-adenosyl-methionine (SAM), which is used in myriad cellular methylation reactions, including all histone methylation reactions. Here, we demonstrate that folate and methionine deficiency led to reduced methylation of lysine 4 of histone H3 (H3K4) in Saccharomyces cerevisiae. The effect of nutritional deficiency on H3K79 methylation was less pronounced, but was exacerbated in S. cerevisiae carrying a hypomorphic allele of Dot1, the enzyme responsible for H3K79 methylation. This result suggested a hierarchy of epigenetic modifications in terms of their susceptibility to nutritional limitations. Folate deficiency caused changes in gene transcription that mirrored the effect of complete loss of H3K4 methylation. Histone methylation was also found to respond to nutritional deficiency in the fission yeast Schizosaccharomyces pombe and in human cells in culture.
authors: Department of Molecular and Cell Biology, University of California, Berkeley, California 94720-3220.
authors: Meru J
authors: MJ
authors: Sadhu
authors: Qiaoning
authors: Q
authors: Guan
authors: Fei
authors: F
authors: Li
authors: Jade
authors: J
authors: Sales-Lee
authors: Anthony T
authors: AT
authors: Iavarone
authors: Ming C
authors: MC
authors: Hammond
authors: W Zacheus
authors: WZ
authors: Cande
authors: Jasper
authors: J
authors: Rine
mesh_terms: D044127:Epigenesis, Genetic; D005260:Female; D005492:Folic Acid / Q000378:metabolism*; D005493:Folic Acid Antagonists / Q000009:adverse effects / Q000627:therapeutic use; D005494:Folic Acid Deficiency / Q000150:complications / Q000235:genetics / Q000378:metabolism; D006657:Histones / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D006801:Humans; D007231:Infant, Newborn; D020014:K562 Cells; D008715:Methionine / Q000378:metabolism*; D008745:Methylation; D009436:Neural Tube Defects / Q000209:etiology / Q000235:genetics / Q000378:metabolism; D011247:Pregnancy; D012436:S-Adenosylmethionine / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D012568:Schizosaccharomyces / Q000235:genetics* / Q000378:metabolism*; D029702:Schizosaccharomyces pombe Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D013045:Species Specificity
chemical_list: D005493:Folic Acid Antagonists; D006657:Histones; D029701:Saccharomyces cerevisiae Proteins; D029702:Schizosaccharomyces pombe Proteins; D012436:S-Adenosylmethionine; D005492:Folic Acid; D008715:Methionine
keywords: S-adenosyl-methionine; Set1; folate; histone methylation; methionine
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