title: A biochemical and histopathologic study showing protection and treatment of gentamicin-induced nephrotoxicity in rabbits using vitamin C.
abstract: Gentamicin and vitamin C have been proposed as nephrotoxic and antioxidant, respectively. This study involved biochemical and histopathologic investigation showing protection and treatment of gentamicin-induced nephrotoxicity in rabbits using vitamin C for 26 days hypothesizing that whether vitamin C would inhibit or decrease the raised serum urea and creatinine levels. This study was conducted on 25 healthy male albino rabbits (average weight 1.5±0.2 kg), classified into 5 groups: group A, B, C, D and E for nephrocurative (study-I) and nephroprotective (study-II) studies. Control group of rabbits (group A) received only the vehicle of gentamicin ampoule. In study-I, gentamicin sulphate (GS 80 mg/kg, i.m.) was administered to group B and C rabbits for ten days, then group C rabbits received vitamin C 250 mg/Kg for remaining 16 days. Group D and E received GS 80 mg/kg and GS 80 mg/kg i.m.-vitamin C 250 mg/kg orally, respectively during whole period (26 days) of study-II. After 26 days, various biochemical parameters, i.e. serum creatinine, blood urea nitrogen (BUN), and serum antioxidant activity, and histopathologic investigations were made. Nephrotoxicity was observed in rabbit groups B, C and D as evident from significant (p<0.05) high levels of serum creatinine and BUN and low serum antioxidant levels as compared to the levels of control group. Decrease in the levels of serum creatinine and BUN along with the increase in serum antioxidant activity was observed after vitamin C treatment in group C. While, renal-protective role of vitamin C was seen in group E as compared to the control. In conclusion, Gentamicin induced nephrotoxicity can be attenuated or treated using vitamin C.
authors: Institute of Pharmacology, Toxicology and Biochemical Pharmaceutics, College of Pharmaceutical Sciences, Zhejiang University Hangzhou, China.
authors: K
authors: K
authors: Rehman
authors: M S H
authors: MS
authors: Akash
authors: S
authors: S
authors: Azhar
authors: S A
authors: SA
authors: Khan
authors: R
authors: R
authors: Abid
authors: A
authors: A
authors: Waseem
authors: G
authors: G
authors: Murtaza
authors: T A
authors: TA
authors: Sherazi
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism / Q000494:pharmacology / Q000627:therapeutic use*; D001205:Ascorbic Acid / Q000494:pharmacology / Q000627:therapeutic use*; D001806:Blood Urea Nitrogen; D003404:Creatinine / Q000097:blood; D005839:Gentamicins / Q000009:adverse effects*; D007668:Kidney / Q000187:drug effects* / Q000473:pathology; D007674:Kidney Diseases / Q000097:blood / Q000139:chemically induced / Q000188:drug therapy* / Q000473:pathology; D008297:Male; D011817:Rabbits; D014508:Urea / Q000097:blood; D014815:Vitamins / Q000494:pharmacology / Q000627:therapeutic use*
chemical_list: D000975:Antioxidants; D005839:Gentamicins; D014815:Vitamins; D014508:Urea; D003404:Creatinine; D001205:Ascorbic Acid
keywords: Blood urea nitrogen; Gentamicin; Nephroprotective and nephrocurative activities; Serum antioxidant activity; Serum creatinine; Vitamin C
references: Yonsei Med J. 2007 Oct 31;48(5):847-55
references: 17963344
references: PLoS One. 2009;4(2):e4409
references: 19197388
references: Clin Chim Acta. 2007 Jan;375(1-2):82-6
references: 16889761
references: Clin Exp Nephrol. 2005 Mar;9(1):24-30
references: 15830269
references: Pharmacol Ther. 2007 Apr;114(1):74-93
references: 17316817
references: J Vet Pharmacol Ther. 2007 Oct;30(5):477-81
references: 17803742
references: Toxicology. 2001 May 11;162(2):81-8
references: 11337108
references: Res Commun Mol Pathol Pharmacol. 1996 Dec;94(3):271-87
references: 9029674
references: Exp Physiol. 2005 Jul;90(4):571-6
references: 15769882
references: Environ Health Perspect. 1984 Aug;57:293-9
references: 6389114
references: Antimicrob Agents Chemother. 1999 May;43(5):1003-12
references: 10223907
references: Mol Cell Biol. 2004 Aug;24(15):6645-52
references: 15254232
references: Proc Natl Acad Sci U S A. 2004 Mar 9;101(10):3691-6
references: 14993613
references: J Clin Pathol. 2001 May;54(5):356-61
references: 11328833
references: Cell Biol Toxicol. 2006 Nov;22(6):455-61
references: 16964585