title: Association of genetic variability in selected regions in visfatin (NAMPT) gene with anthropometric parameters and dietary composition in obese and non-obese Central-European population.
abstract: OBJECTIVE
  Visfatin (NAMPT/PBEF) is a recently identified adipocytokine which harbors strong insulin-mimetic activity and was reported to be associated with obesity. However, nothing is known about whether visfatin is related to specific nutritional behavior which may result in obesity development. This is the first study focusing on genetic variability of the visfatin gene and its association with circulating visfatin, anthropometric parameters and dietary composition.
  
  
  METHODS
  We analyzed a total of 11 exons and adjacent non-coding regions of the NAMPT gene in 20 extremely obese Czech individuals (mean BMI 52.2±5.0 SD) using direct sequencing and a frequency of rs2302559 was established in the validation cohort of another 605 individuals with completed 7-day food records and complex anthropometric measurements. Serum levels of visfatin, leptin and leptin-receptor were measured in all sequenced individuals and in part of the validation cohort.
  
  
  RESULTS
  Three common polymorphisms were identified, two in non-coding regions (rs78411774 A/C, rs71564769 A/C) and one synonymous SNP in exon 7 (rs2302559 A/G). The rs2302559 showed significant correlation with visfatin serum level throughout the entire study cohort (p<0.001); there was a significant tendency toward higher visfatin levels in G allele carriers with GG homozygotes having the highest visfatin serum levels. Furthermore, a negative correlation was observed between visfatin and leptin serum level (p=0.01). No association between investigated SNPs and anthropometric parameters or native dietary composition was observed.
  
  
  CONCLUSIONS
  This is the first study to demonstrate that the rs2302559 polymorphism in the PBEF gene is related to circulating levels of visfatin. As the SNP is synonymous, we hypothesize it might be linked to another SNP in the PBEF gene which controls visfatin serum levels.
authors: Department of Pathological Physiology, Faculty of Medicine, Masaryk University, Kamenice 5, Brno, Czech Republic.
authors: Jiri
authors: J
authors: Stastny
authors: Julie
authors: J
authors: Bienertova-Vasku
authors: Josef
authors: J
authors: Tomandl
authors: Marie
authors: M
authors: Tomandlova
authors: Filip
authors: F
authors: Zlamal
authors: Martin
authors: M
authors: Forejt
authors: Zbynek
authors: Z
authors: Splichal
authors: Anna
authors: A
authors: Vasku
mesh_terms: D000886:Anthropometry; D001786:Blood Glucose / Q000378:metabolism*; D015992:Body Mass Index; D016207:Cytokines / Q000235:genetics* / Q000378:metabolism; D018153:Czech Republic / Q000453:epidemiology; D004032:Diet; D015930:Diet Records; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D054409:Nicotinamide Phosphoribosyltransferase / Q000235:genetics* / Q000378:metabolism; D009765:Obesity / Q000453:epidemiology / Q000235:genetics* / Q000378:metabolism; D020641:Polymorphism, Single Nucleotide; D017421:Sequence Analysis; D013851:Thinness; D044465:White People / Q000235:genetics*
chemical_list: D001786:Blood Glucose; D016207:Cytokines; D054409:Nicotinamide Phosphoribosyltransferase; C085455:nicotinamide phosphoribosyltransferase, human
keywords: Anthropometric parameters; Dietary composition; Extreme obesity; NAMPT; SNP; Visfatin