title: A facile label-free electrochemiluminescence biosensor for target protein specific recognition based on the controlled-release delivery system.
abstract: This paper described a novel label-free electrochemiluminescence assay for target protein based on a controlled delivery system. Iron oxide magnetic mesoporous silica nanocontainers were prepared by using a general procedure. The prepared magnetic mesoporous silica nanocontainers were applied to load the guest molecules [Ru(bpy)3](2+). Aptamers were used as gatekeepers on the pore outlets of the nanocontainers. In the presence of target proteins, the specific aptamer-protein interactions were employed as triggers for uncapping the pores and releasing the guest molecules from the nanocontainers. The amount of the guest molecule [Ru(bpy)3](2+) released from the magnetic mesoporous silica nanocontainers was monitored by the electrochemiluminescence assay. The results show that the releasing amount of [Ru(bpy)3](2+) is proportional to the thrombin concentration in the range of 0.6 pM-0.8 nM with a detection limit of 0.5 pM (S/N=3). The present work demonstrates that the fabricated nanocontainer using aptamer as the cap is a highly sensitive and selective key-in lock gating system for the label-free ECL biosensor.
authors: Shandong Provincial Key Laboratory of Biochemical Analysis, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, Shandong, PR China. yangxiaoyan_zh@126.com
authors: Xiaoyan
authors: X
authors: Yang
authors: Aiguo
authors: A
authors: Wang
authors: Jilong
authors: J
authors: Liu
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry; D015374:Biosensing Techniques; D003692:Delayed-Action Preparations; D016503:Drug Delivery Systems; D004563:Electrochemistry; D052203:Ferrosoferric Oxide / Q000737:chemistry; D008163:Luminescent Measurements; D049329:Nanostructures / Q000737:chemistry; D009942:Organometallic Compounds / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry; D013917:Thrombin / Q000032:analysis* / Q000737:chemistry
chemical_list: D052157:Aptamers, Nucleotide; D003692:Delayed-Action Preparations; D009942:Organometallic Compounds; C415768:tris(2,2'-bipyridyl)ruthenium(II); D012822:Silicon Dioxide; D013917:Thrombin; D052203:Ferrosoferric Oxide
keywords: Controlled-delivery; Electrochemiluminescence; Iron oxide; Label-free; Mesoporous silica