title: Effects of genistein and hesperidin on biomarkers of heat stress in broilers under persistent summer stress.
abstract: This study investigated the supplemental effects of the flavonoids genistein and hesperidin for biomarkers of heat stress in broilers reared under persistent summer stress. A total of 360 one-day-old, mixed-sex broiler chickens were divided into 6 treatment groups: control or supplemented with 5 mg of genistein•kg of feed(-1), 20 mg of hesperidin•kg of feed(-1), or a mixture of genistein and hesperidin (1:4) at a dosage of 5 mg•kg(-1), 10 mg•kg(-1), and 20 mg•kg(-1) of feed. Broilers were slaughtered at 42 d and samples were analyzed for hematological profile, creatine kinase, lactate dehydrogenase, and heat shock protein 70 mRNA levels. Results showed that dietary genistein and hesperidin improved (P < 0.05) the weekly performance of broilers particularly during the finisher period. The circulating heterophils and heterophil-to-lymphocyte ratios were found to decrease (P < 0.01) in the treated groups. Moreover, biomarkers of heat stress including the level of creatine kinase, lactate dehydrogenase, and heat shock protein 70 mRNA of breast muscle was also changed (P < 0.01) positively by the dietary compounds with pronounced effects of combined treatments. These findings suggested that genistein and hesperidin could be a prime strategy to ameliorate summer stress effects in broilers; and a combination of both compounds may lead to mutual synergistic effects. It could be suggested that dietary use of both genistein and hesperidin as a feed supplement may offer a potential nutritional strategy in tropical and subtropical regions to overcome the deleterious effects of persistent summer stress in broiler production.
authors: Laboratory of Gastrointestinal Microbiology, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China. drasgharkamboh@yahoo.com
authors: A A
authors: AA
authors: Kamboh
authors: S Q
authors: SQ
authors: Hang
authors: M
authors: M
authors: Bakhetgul
authors: W-Y
authors: WY
authors: Zhu
mesh_terms: D000821:Animal Feed / Q000032:analysis; D000822:Animal Husbandry; D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D015415:Biomarkers / Q000378:metabolism; D002645:Chickens / Q000502:physiology*; D003402:Creatine Kinase / Q000378:metabolism; D004032:Diet / Q000662:veterinary; D019587:Dietary Supplements / Q000032:analysis; D004338:Drug Combinations; D005260:Female; D019833:Genistein / Q000008:administration & dosage / Q000378:metabolism*; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism; D018869:Heat-Shock Response / Q000187:drug effects*; D006569:Hesperidin / Q000008:administration & dosage / Q000378:metabolism*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008297:Male; D010369:Pectoralis Muscles / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D004338:Drug Combinations; D018840:HSP70 Heat-Shock Proteins; D019833:Genistein; D006569:Hesperidin; D007770:L-Lactate Dehydrogenase; D003402:Creatine Kinase