title: Flavonoid compounds identified in Alchemilla L. species collected in the north-eastern Black Sea region of Turkey.
abstract: This study identified flavonoid glycosides in species of the genus Alchemilla, A. procerrima, A. stricta, A. hirtipedicellata and A. sericata. A. procerrima is an endemic species for Turkey. After detailed investigation, flavonoid compounds of the species were identified for the first time. In this study, flavonoid compounds were determined by using two different chromatography techniques, TLC and HPLC. The following flavonoid compounds were identified from the Alchemilla species studied. They are as follows: orientin (luteolin-8-C-glucoside) Rf: 0,70, vitexin (apigenin-8-C-glucoside) Rf: 0,77 as flavone-C-glycoside, rutin (quercetin-3-O-rutinoside) Rf: 0,44, hyperoside (quercetin-3-O-galactoside) Rf: 0,65, isoquercetin (quercetin-3-O-glucopyranoside) Rf: 0,72, quercitrin (quercetin-3-O-rhamnoside) Rf: 0,84 as flavonol-O-glycoside. Three more folavonoids with Rf values of Rf₁=0,36, Rf₂=0,54 and Rf₃=0,68 were also identified for the first time in this study. Rutin (quercetin-3-O-rutinoside) and the flavonoid glycoside, shown as Rf₂ were found in all species. Quercitrin and isoquercetin were determined in all analysed species but A. procerrima. Hyperoside was identified in all species except for A. stricta. Vitexin was determined only in A.stricta. Orientin was determined in A. procerrima and A. stricta, but could not be determined in A. sericata and A. hirtpedicellata. Unknown flavonoid with Rf₁ and Rf₃ were determined outside of A. sericata. Description of these compounds in Turkish Alchemilla plants for the first time should be viewed as a discovery of an important chemosystematic feature.
authors: Department of Biology, Faculty of Science and Art, Bingöl University, 12000 Bingöl, Turkey. b_kaya_tr@yahoo.com
authors: Bülent
authors: B
authors: Kaya
authors: Yusuf
authors: Y
authors: Menemen
authors: Fatma Zerrin
authors: FZ
authors: Saltan
mesh_terms: D031982:Alchemilla / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D005419:Flavonoids / Q000032:analysis*; D006027:Glycosides / Q000032:analysis*; D010936:Plant Extracts / Q000737:chemistry*; D014421:Turkey
chemical_list: D005419:Flavonoids; D006027:Glycosides; D010936:Plant Extracts
keywords: Alchemilla L.; Flavone-C-glycoside; Flavonol-O-glycoside; HPLC; Rosaceae; TLC
references: Fitoterapia. 2000 Aug;71(4):463-4
references: 10925029
references: Cancer Res. 1988 Oct 15;48(20):5754-8
references: 3139283
references: J Chromatogr A. 2005 Oct 7;1090(1-2):193-6
references: 16196150
references: Planta Med. 1989 Jun;55(3):235-41
references: 2662231
references: J Asian Nat Prod Res. 2003 Jun;5(2):131-6
references: 12765197
references: Biochem Syst Ecol. 2001 Oct;29(9):959-962
references: 11445296
references: J Pharm Pharmacol. 1988 Nov;40(11):787-92
references: 2907559
references: Free Radic Res Commun. 1987;2(4-6):289-94
references: 3504810
references: Mol Phylogenet Evol. 2008 Jun;47(3):1030-44
references: 18479944