title: Effects of Mn-DPDP and manganese chloride on hemodynamics and glucose tolerance in anesthetized rats.
abstract: BACKGROUND
  Previous studies have demonstrated that magnetic resonance imaging may be a method of choice to visualize transplanted pancreatic islets. However, contrast agents may interfere with microcirculation and affect graft function.
  
  
  OBJECTIVE
  To evaluate the effects manganese-containing contrast media on regional blood flow and glucose tolerance.
  
  
  METHODS
  Anesthetized rats were injected intravenously with MnCl2 (10 µM/kg body weight) or Mn-DPDP (Teslascan™; 5 µM/kg body weight). Blood flow measurements were made with a microsphere technique 10 min later. In separate animals vascular arteriolar reactivity in isolated, perfused islets was examined. Furthermore, an intraperitoneal glucose tolerance test was performed in separate rats.
  
  
  RESULTS
  Glucose tolerance was unaffected by both agents. No changes in regional blood flow were seen after administration of Mn-DPDP, except for an increase in arterial liver blood flow. MnCl2 increased all blood flow values except that of the kidney. MnCl2, but not Mn-DPDP, caused a vasoconstriction in isolated rat islet arterioles but only at very high doses.
  
  
  CONCLUSIONS
  Mn-DPDP administration does not affect glucose tolerance or regional blood flow, besides an increase in arterial hepatic blood flow, and may therefore be suitable for visualization of islets.
authors: Department of Medical Cell Biology, Uppsala University, Uppsala, Sweden.
authors: Xiang
authors: X
authors: Gao
authors: Monica
authors: M
authors: Sandberg
authors: My
authors: M
authors: Quach
authors: Birgitta
authors: B
authors: Bodin
authors: Lars
authors: L
authors: Johansson
authors: Leif
authors: L
authors: Jansson
mesh_terms: D000818:Animals; D002712:Chlorides / Q000494:pharmacology*; D003287:Contrast Media / Q000494:pharmacology*; D004492:Edetic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D005951:Glucose Tolerance Test; D006439:Hemodynamics / Q000187:drug effects*; D007515:Islets of Langerhans / Q000098:blood supply* / Q000187:drug effects*; D008279:Magnetic Resonance Imaging / Q000379:methods*; D008297:Male; D017895:Manganese Compounds / Q000494:pharmacology*; D008863:Microspheres; D011732:Pyridoxal Phosphate / Q000031:analogs & derivatives* / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012039:Regional Blood Flow / Q000187:drug effects
chemical_list: D002712:Chlorides; D003287:Contrast Media; D017895:Manganese Compounds; D011732:Pyridoxal Phosphate; D004492:Edetic Acid; C060076:N,N'-bis(pyridoxal-5-phosphate)ethylenediamine-N,N'-diacetic acid; C025340:manganese chloride
keywords: Pancreatic islets; glucose tolerance; manganese; radio contrast agents; regional blood flow