title: Convenient detection of E. coli in Ringer's solution.
abstract: An easy and inexpensive detection method for DNA hybridization assays combining magnetic beads and enzymatically generated silver nanoparticles is introduced. The main advantage of this approach is the possibility to distinguish between positive and negative test results with the naked eye. In the case of complementary DNA sequences the sample will turn black within a few minutes, allowing readout without any hardware. In order to illustrate the applicability of the assay genomic DNA isolated from E. coli contaminated Ringer's solution was used for testing the sensitivity as well as specificity.
authors: Institute of Photonic Technology Jena, Albert-Einstein-Straße 9, 07745 Jena, Germany. juergen.popp@ipht-jena.de.
authors: Martha
authors: M
authors: Schwarz
authors: Susanne
authors: S
authors: Pahlow
authors: Thomas
authors: T
authors: Bocklitz
authors: Carolin
authors: C
authors: Steinbrücker
authors: Dana
authors: D
authors: Cialla
authors: Karina
authors: K
authors: Weber
authors: Jürgen
authors: J
authors: Popp
mesh_terms: D015342:DNA Probes / Q000032:analysis / Q000191:economics / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification*; D004926:Escherichia coli / Q000235:genetics / Q000302:isolation & purification*; D007552:Isotonic Solutions / Q000032:analysis*; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000191:economics; D016133:Polymerase Chain Reaction / Q000191:economics / Q000379:methods; D000077331:Ringer's Solution; D012834:Silver / Q000737:chemistry* / Q000191:economics
chemical_list: D015342:DNA Probes; D004269:DNA, Bacterial; D007552:Isotonic Solutions; D012834:Silver; D000077331:Ringer's Solution