title: miR-205 is frequently downregulated in prostate cancer and acts as a tumor suppressor by inhibiting tumor growth.
abstract: The purpose of this study was to elucidate the molecular mechanisms of microRNA-205 (miR-205) as a tumor suppressor in prostate cancer (PCa). In the present study, microRNA microarray analysis suggested that the expression of miR-205 was significantly decreased in advanced PCa compared with early PCa. Real-time PCR analysis also indicated that miR-205 expression was significantly decreased in PCa tissues compared with non-cancerous tissues. Moreover, the expression of miR-205 has been demonstrated to be associated with the clinicopathological stage and total/free prostate-specific antigen (PSA) level of PCa. Functional analyses showed that both the overexpression of miR-205 and the knockdown of c-SRC in PCa cell lines could inhibit cell growth, colony formation, migration, invasion and the cell cycle as well as induce cell apoptosis in vitro. Furthermore, over-expressing miR-205 reduced tumorigenicity in vivo. Through a luciferase activity assay and Western blotting, c-SRC was identified as a target of miR-205 in cells. The overexpression of miR-205 suppressed c-SRC and its downstream signaling molecules, including FAK, p-FAK, ERK1/2 and p-ERK1/2, and attenuated cell proliferation, invasion and tumor growth.
authors: Ning
authors: N
authors: Wang
authors: Qi
authors: Q
authors: Li
authors: Ning-Han
authors: NH
authors: Feng
authors: Gong
authors: G
authors: Cheng
authors: Zhao-Long
authors: ZL
authors: Guan
authors: Yang
authors: Y
authors: Wang
authors: Chao
authors: C
authors: Qin
authors: Chang-Jun
authors: CJ
authors: Yin
authors: Li-Xin
authors: LX
authors: Hua
mesh_terms: D000818:Animals; D002455:Cell Division / Q000235:genetics*; D045744:Cell Line, Tumor; D015536:Down-Regulation; D005434:Flow Cytometry; D016147:Genes, Tumor Suppressor; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D035683:MicroRNAs / Q000235:genetics*; D011471:Prostatic Neoplasms / Q000235:genetics* / Q000473:pathology
chemical_list: D035683:MicroRNAs; C534425:MIR205, human
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