title: Molecular characterization of echovirus 30-associated outbreak of aseptic meningitis in Guangdong in 2012.
abstract: BACKGROUND
  Evaluation of the primary etiologic agents that cause aseptic meningitis outbreaks may provide valuable information regarding the prevention and management of aseptic meningitis. An outbreak of aseptic meningitis occurred from May to June, 2012, in Guangdong Province, China. In order to determine the etiologic agent, CSF specimens from 121 children hospitalized for aseptic meningitis at Luoding People's Hospital of Guangdong Province were tested for virus isolation and identification.
  
  
  RESULTS
  Enterovirus RNA was positive in 62.0% of 121 CSF sspecimens by real-time polymerase chain reaction (RT-PCR). Amplification and sequencing of the VP1 region of enterovirus isolates revealed Echovirus 30 (E30) was the most common isolated serotype (80% of 40 enterovirus strains).For the molecular characterization of the E30 isolates, the VP1 gene sequence of 20 Luoding E30 isolates was compared pairwise using the MegAlign with reference strains from GenBank. The pairwise comparison of the nucleotide sequences of the VP1 genes demonstrated that the sequences of the strains differed from those of lineage groups C, D, E, F, and G. Reconstruction of the phylogenetic tree based on the VP1 nucleotide sequences resulted in a monophyletic tree, with seven clustered lineage groups. Most of the isolates were segregated from other lineage groups. Four E30 isolates causing this outbreak aggregated into the Lineage A cluster which was derived from E30 strains that circulated in other regions of China from 2003-2010.
  
  
  CONCLUSIONS
  This study demonstrated the Luoding strains were a distinct lineage of E30, and a probable cause of this outbreak. The study also demonstrated that different E30 variants existed in the local meningitis outbreak.
authors: Hong
authors: H
authors: Xiao
authors: Dawei
authors: D
authors: Guan
authors: Ruiyu
authors: R
authors: Chen
authors: Pingxi
authors: P
authors: Chen
authors: Corina
authors: C
authors: Monagin
authors: Wei
authors: W
authors: Li
authors: Juan
authors: J
authors: Su
authors: Cong
authors: C
authors: Ma
authors: Wanli
authors: W
authors: Zhang
authors: Key Laboratory for Emergency Pathogen Detection, Guangdong Provincial Center for Disease Control and Prevention, Guangzhou 511430, Guangdong, China. kecw2011@sina.com.
authors: Changwen
authors: C
authors: Ke
mesh_terms: D000293:Adolescent; D000595:Amino Acid Sequence; D002555:Cerebrospinal Fluid / Q000821:virology; D002648:Child; D002675:Child, Preschool; D002681:China / Q000453:epidemiology; D016000:Cluster Analysis; D004196:Disease Outbreaks; D004457:Echovirus Infections / Q000453:epidemiology* / Q000821:virology*; D029822:Enterovirus B, Human / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D005260:Female; D005838:Genotype; D006801:Humans; D007223:Infant; D008297:Male; D008582:Meningitis, Aseptic / Q000453:epidemiology* / Q000821:virology*; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D015678:Viral Structural Proteins / Q000235:genetics
chemical_list: D012367:RNA, Viral; D015678:Viral Structural Proteins
references: J Med Virol. 2002 Dec;68(4):558-67
references: 12376964
references: Semin Pediatr Infect Dis. 2002 Jan;13(1):40-7
references: 12118843
references: J Gen Virol. 2007 Jan;88(Pt 1):166-176
references: 17170449
references: J Clin Microbiol. 1999 Dec;37(12):3928-33
references: 10565909
references: Jpn J Infect Dis. 2007 Feb;60(1):66-8
references: 17314434
references: J Clin Microbiol. 2002 Dec;40(12):4439-44
references: 12454133
references: J Microbiol Biotechnol. 2010 Mar;20(3):643-9
references: 20372040
references: Neurol Clin. 2008 Aug;26(3):635-55, vii-viii
references: 18657719
references: J Clin Microbiol. 2008 Jan;46(1):87-96
references: 17977989
references: J Gen Virol. 2001 Jan;82(Pt 1):79-91
references: 11125161
references: J Med Virol. 2003 Jul;70(3):420-9
references: 12767006
references: J Clin Microbiol. 2006 Aug;44(8):2698-704
references: 16891480
references: Zhonghua Liu Xing Bing Xue Za Zhi. 2006 Sep;27(9):793-7
references: 17299967
references: J Virol. 2009 Mar;83(5):2109-18
references: 19091869
references: J Gen Virol. 2003 Apr;84(Pt 4):827-836
references: 12655083
references: J Virol. 2002 May;76(10):4940-9
references: 11967311
references: J Clin Virol. 2003 Apr;26(3):375-7
references: 12637088
references: Arch Virol. 2001;146(3):521-37
references: 11338388
references: J Clin Microbiol. 2006 Nov;44(11):4142-8
references: 16957029