title: Comparison of activation ability between feline and bovine oocytes.
abstract: Research comparing the activation sensitivity of oocytes to chemical treatment among mammalian species remains limited. We compared the activation ability of oocytes from bovine and feline ovaries when treated with ethanol alone, with ethanol and cycloheximide, and without any chemical treatment; the oocytes were then cultured for 72 h. After in vitro maturation (IVM), 5% of feline oocytes were activated and 1% were cleaved, whereas there were no prematurely activated bovine oocytes. Activation rates with ethanol and ethanol/cycloheximide were significantly higher (P < 0.01) in bovine oocytes than in feline oocytes (74.2% vs. 34.1% and 86.3% vs. 52.5%, respectively). Thus, our findings indicate that feline oocytes can be prematurely activated by the end of IVM, and that bovine oocytes may have a higher sensitivity of parthenogenetic activation to chemical treatment than do feline oocytes.
authors: Yamaguchi University The United Graduate School of Veterinary Science Yamaguchi 753-8515 Japan.
authors: Fuminori
authors: F
authors: Tanihara
authors: Yukine
authors: Y
authors: Kaedei
authors: Zhao
authors: Z
authors: Namula
authors: Vien Viet
authors: VV
authors: Luu
authors: Yoko
authors: Y
authors: Sato
authors: Manita
authors: M
authors: Wittayarat
authors: Masayasu
authors: M
authors: Taniguchi
authors: Takeshige
authors: T
authors: Otoi
mesh_terms: D000818:Animals; D002415:Cats; D002417:Cattle; D003513:Cycloheximide; D000431:Ethanol; D005307:Fertilization in Vitro; D009865:Oocytes; D010312:Parthenogenesis
chemical_list: D000431:Ethanol; D003513:Cycloheximide
keywords: Feline; activation; bovine; nuclear transfer; oocyte; parthenogenesis