title: Prenatal testosterone exposure worsen the reproductive performance of male rat at adulthood.
abstract: The reproductive system is extremely susceptible to environmental insults, for example exogenous steroids during gestational development and differentiation. Experimental induction of androgen excess during prenatal life in female animal models reprograms their reproductive physiology, however the fetal programming of the male reproductive system by androgen excess has not been well studied. We aimed to determine the effect of prenatal exposure of two different doses of testosterone on different gestational days, on the male reproductive system using a rat model. Sixteen pregnant rats were randomly divided into two experimental groups and two control groups. Experimental group І were subcutaneously injected with 3 mg free testosterone on gestational days 16-19 and its controls received solvent for that time; experimental group П were subcutaneously injected with 20 mg free testosterone on day 20 of gestational period and its controls received solvent at the same time. The reproductive system morphology and function of 32 male offspring of these study groups were compared at days 6-30-60 of age and after puberty. The anogenital distance of the male offspring of both experimental groups had no significant differences on the different days of measurement, compared with controls. In the offspring of experimental group І, the testes weight, number of Sertoli, Spermatocyte and Spermatid cells, sperm count and motility and the serum concentration of testosterone after puberty were significantly decreased; except for reduction of sperm motility (p< 0.01), the other effects were not observed in the offspring of experimental group ІІ. In summary, our data show that prenatal exposure of male rat fetuses to excess testosterone disrupted reproductive function, an effect highly dependent on the time, duration and level of exposure. It seems that the reproductive system in individuals exposed to high levels of androgens during fetal life should be evaluated at puberty and likely to be treated.
authors: Reproduction Endocrinology Research Center, Research Institute for Endocrine Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors: Fahimeh
authors: F
authors: Ramezani Tehrani
authors: Mahsa
authors: M
authors: Noroozzadeh
authors: Saleh
authors: S
authors: Zahediasl
authors: Asghar
authors: A
authors: Ghasemi
authors: Abbas
authors: A
authors: Piryaei
authors: Fereidoun
authors: F
authors: Azizi
mesh_terms: D000818:Animals; D001835:Body Weight; D005260:Female; D006728:Hormones / Q000097:blood; D008297:Male; D018811:Maternal Exposure; D009929:Organ Size; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects; D051381:Rats; D012098:Reproduction / Q000187:drug effects*; D013076:Sperm Count; D013081:Sperm Motility; D013739:Testosterone / Q000008:administration & dosage*
chemical_list: D006728:Hormones; D013739:Testosterone
references: Endocrinology. 2008 Oct;149(10):5280-7
references: 18566125
references: Environ Toxicol. 2014 May;29(5):534-44
references: 22489061
references: Endocrinology. 1999 Sep;140(9):3938-46
references: 10465262
references: Exp Clin Endocrinol Diabetes. 2006 Apr;114(4):208-14
references: 16705555
references: J Urol. 1998 Feb;159(2):441-3
references: 9649259
references: Endocr Rev. 2001 Jun;22(3):319-41
references: 11399747
references: Endocrinology. 2005 Jun;146(6):2674-83
references: 15761038
references: Eur J Endocrinol. 2009 May;160(5):739-46
references: 19218283
references: J Clin Invest. 2008 Apr;118(4):1479-90
references: 18340380
references: Biol Res. 2007;40(3):329-38
references: 18449460
references: PLoS One. 2012;7(5):e37658
references: 22655062
references: Reprod Biol Endocrinol. 2011 Jun 24;9:94
references: 21702915
references: Nature. 1970 Sep 19;227(5264):1217-9
references: 5452809
references: Am J Physiol Endocrinol Metab. 2010 Dec;299(6):E998-E1005
references: 20858754
references: Mol Cell Endocrinol. 2000 May 25;163(1-2):3-9
references: 10963867
references: J Assist Reprod Genet. 2006 Sep-Oct;23(9-10):377-80
references: 17033937
references: Endocrinology. 2008 Dec;149(12):6444-8
references: 18669598
references: Endocrinology. 2004 Jan;145(1):318-29
references: 14551232
references: Toxicol Pathol. 1997 Mar-Apr;25(2):119-31
references: 9125770
references: J Physiol Sci. 2012 Mar;62(2):123-31
references: 22234789
references: Endocrinology. 1988 Mar;122(3):787-94
references: 3125042
references: Toxicol Sci. 2007 Apr;96(2):335-45
references: 17218470
references: Endocr J. 2010;57(3):201-9
references: 20057162
references: Endocrinology. 2007 May;148(5):2027-36
references: 17289843
references: Endocrinology. 2007 Aug;148(8):3781-91
references: 17495003
references: Endocrinology. 2003 Jan;144(1):138-45
references: 12488339
references: Hum Reprod. 2002 Oct;17(10):2573-9
references: 12351531
references: Hum Reprod Update. 2005 Jul-Aug;11(4):357-74
references: 15941725
references: Reproduction. 2011 Jul;142(1):167-73
references: 21493716
references: Biol Reprod. 2001 Jul;65(1):318-32
references: 11420255
references: J Clin Endocrinol Metab. 2008 Sep;93(9):3318-24
references: 18544620