title: Thioredoxin-interacting protein regulates glucose metabolism and affects cytoplasmic streaming in mouse oocytes.
abstract: Thioredoxin-interacting protein (Txnip) regulates intracellular redox state and prompts oxidative stress by binding to and inhibiting Thioredoxin (Trx). In addition, via a Trx-independent mechanism, Txnip regulates glucose metabolism and thus maintains intracellular glucose levels. Previously, we found Txnip mRNA highly expressed in immature germinal vesicle (GV) oocytes, but currently there is no report describing the role of Txnip in oocytes. Therefore, we conducted the present study to determine the function of Txnip in mouse oocytes' maturation and meiosis by using RNA interference (RNAi) method. Upon specific depletion of Txnip, 79.5% of oocytes were arrested at metaphase I (MI) stage. Time-lapse video microscopy analysis revealed that the formation of granules in the oocyte cytoplasm increased concurrent with retarded cytoplasmic streaming after Txnip RNAi treatment. Txnip RNAi-treated oocytes had upregulated glucose uptake and lactate production. To confirm the supposition that mechanism responsible for these observed phenomena involves increased lactate in oocytes, we cultured oocytes in high lactate medium and observed the same increased granule formation and retarded cytoplasmic streaming as found by Txnip RNAi. The MI-arrested oocytes exhibited scattered microtubules and aggregated chromosomes indicating that actin networking was disturbed by Txnip RNAi. Therefore, we conclude that Txnip is a critical regulator of glucose metabolism in oocytes and is involved in maintaining cytoplasmic streaming in mouse oocytes.
authors: Department of Biomedical Science, College of Life Science, CHA University, Seoul, Korea.
authors: Su-Yeon
authors: SY
authors: Lee
authors: Hyun-Seo
authors: HS
authors: Lee
authors: Eun-Young
authors: EY
authors: Kim
authors: Jung-Jae
authors: JJ
authors: Ko
authors: Tae Ki
authors: TK
authors: Yoon
authors: Woo-Sik
authors: WS
authors: Lee
authors: Kyung-Ah
authors: KA
authors: Lee
mesh_terms: D008841:Actin Cytoskeleton / Q000648:ultrastructure; D000818:Animals; D002352:Carrier Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D033481:Chromosomes, Mammalian / Q000648:ultrastructure; D003593:Cytoplasm / Q000378:metabolism / Q000648:ultrastructure; D005260:Female; D015870:Gene Expression; D005947:Glucose / Q000378:metabolism*; D019344:Lactic Acid / Q000096:biosynthesis; D008540:Meiosis; D008677:Metaphase; D051379:Mice; D008813:Mice, Inbred ICR; D008870:Microtubules / Q000648:ultrastructure; D009865:Oocytes / Q000166:cytology / Q000378:metabolism* / Q000648:ultrastructure; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism; D013879:Thioredoxins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D059008:Time-Lapse Imaging; D014741:Video Recording
chemical_list: D002352:Carrier Proteins; D034741:RNA, Small Interfering; C515559:Txn1 protein, mouse; C445672:Txnip protein, mouse; D019344:Lactic Acid; D013879:Thioredoxins; D005947:Glucose
references: Nat Cell Biol. 2011 Aug 28;13(10):1252-8
references: 21874009
references: FASEB J. 2006 Jan;20(1):121-3
references: 16254043
references: Biol Reprod. 2009 Sep;81(3):497-506
references: 19439730
references: Nature. 2005 Aug 11;436(7052):812-8
references: 16015286
references: Biol Reprod. 2008 Dec;79(6):1192-201
references: 18753614
references: Mol Reprod Dev. 2013 Feb;80(2):166-82
references: 23280668
references: Curr Opin Cell Biol. 2003 Dec;15(6):654-63
references: 14644189
references: Reproduction. 2005 Dec;130(6):791-9
references: 16322539
references: Trends Cell Biol. 1996 Jan;6(1):22-8
references: 15157528
references: Am J Physiol Endocrinol Metab. 2012 Jun 15;302(12):E1511-8
references: 22473000
references: Semin Reprod Med. 2000;18(2):123-31
references: 11256162
references: J Biol Chem. 2009 Sep 11;284(37):24996-5003
references: 19605364
references: Proc Natl Acad Sci U S A. 2011 Jul 19;108(29):11900-5
references: 21730185
references: J Biol Chem. 2008 Jan 25;283(4):2397-406
references: 17998203
references: Semin Reprod Med. 2009 Jan;27(1):32-42
references: 19197803
references: Fertil Steril. 2005 Apr;83 Suppl 1:1293-6
references: 15831304
references: Development. 2007 Jun;134(12):2227-36
references: 17507392
references: Biochem J. 1996 Apr 15;315 ( Pt 2):455-60
references: 8615814
references: J Lipid Res. 2005 Jan;46(1):123-34
references: 15520447
references: J Nutr. 2004 Jun;134(6):1475-80
references: 15173414
references: PLoS Med. 2007 May;4(5):e158
references: 17472435
references: Curr Biol. 2008 Oct 14;18(19):1514-9
references: 18848445
references: J Biol Chem. 1999 Jul 30;274(31):21645-50
references: 10419473
references: Proc Natl Acad Sci U S A. 2008 Mar 11;105(10):3663-7
references: 18310326
references: Proc Natl Acad Sci U S A. 2012 Jul 17;109(29):11705-10
references: 22753521
references: Reprod Med Biol. 2004 Dec 03;3(4):231-236
references: 29699201
references: J Immunol. 2000 Jun 15;164(12):6287-95
references: 10843682
references: J Biochem Biophys Methods. 2005 Sep 30;64(3):207-15
references: 16182371
references: FASEB J. 2010 Jul;24(7):2314-24
references: 20154267
references: Front Biosci. 2002 Sep 01;7:d1990-6
references: 12161345
references: Biochem Biophys Res Commun. 2000 May 19;271(3):796-800
references: 10814541
references: Dev Biol. 2002 Jan 1;241(1):1-23
references: 11784091
references: Nat Rev Mol Cell Biol. 2013 Mar;14(3):141-52
references: 23429793
references: J Cell Biol. 1988 May;106(5):1545-52
references: 3372589
references: J Biol Chem. 2006 Aug 4;281(31):21884-21891
references: 16766796
references: J Cell Sci. 2003 Jan 15;116(Pt 2):211-6
references: 12482907
references: Trends Endocrinol Metab. 2009 Dec;20(10):483-9
references: 19811929
references: Diabetes. 2008 Apr;57(4):938-44
references: 18171713
references: IUBMB Life. 2001 Jan;51(1):5-10
references: 11419697
references: Proc Natl Acad Sci U S A. 2010 Oct 12;107(41):17639-44
references: 20876089
references: Circ Res. 2007 Dec 7;101(12):1216-8
references: 18063816