title: Isolation of Extended Spectrum β-lactamase (ESBL) Producing Bacteria from Urban Surface Waters in Malaysia.
abstract: BACKGROUND
  This was a preliminary study to test for the presence of multiple antibiotic-resistant extended spectrum β-lactamase (ESBL) producing bacteria in Malaysian urban surface waters. Although the literature review revealed several published papers on clinical ESBL isolates in Malaysia, none were found on ESBL isolates obtained from local surface waters.
  
  
  METHODS
  Isolated bacterial species were tested for resistance to cefotaxime, amoxicillin/clavulanate and aztreonam, and susceptibility to imipenem and meropenem using antibiotic susceptibility testing (AST) by disc diffusion. This served as a screening step to detect bacteria that could be potential ESBL species. 16S ribose ribonucleic acid (rRNA) polymerase chain reaction (PCR) testing with two clusters of bla (β-lactamase) gene primers was used to test for the bla genes CTX-M (Groups 1, 2, 9), OXA-1, SHV and TEM.
  
  
  RESULTS
  A total of 19 isolates were found, possessing at least one of the bla genes tested for. There was a relatively high occurrence of CTX-M genes (84.2%) among these, followed by TEM genes (47.4%). The isolates were identified as Enterobacteriaceae (89.5%), predominantly Escherichia coli and Klebsiella pneumoniae.
  
  
  CONCLUSIONS
  There appears to be a high occurrence of ESBL-bacteria in local surface waters, among these being opportunistic pathogens. The persistence and spread of these species in the environment poses a threat to exposed human populations.
authors: School of Science, Monash University Sunway Campus, Jalan Lagoon Selatan, Bandar Sunway, 46150, Selangor Darul Ehsan.
authors: Shehani
authors: S
authors: Tissera
authors: Sui Mae
authors: SM
authors: Lee
keywords: antibiotic resistance; bla (β-lactamase) gene; extended spectrum β-lactamase (ESBL); opportunistic pathogen; surface water
references: J Antimicrob Chemother. 2001 Aug;48(2):321-2
references: 11481313
references: Indian J Med Microbiol. 2004 Oct-Dec;22(4):255-7
references: 17642750
references: Chirurg. 2009 Jun;80(6):527-36
references: 19219416
references: Appl Environ Microbiol. 2011 Oct;77(20):7142-6
references: 21685166
references: Antimicrob Agents Chemother. 1995 Jun;39(6):1211-33
references: 7574506
references: Antimicrob Agents Chemother. 2004 Nov;48(11):4263-70
references: 15504851
references: Clin Infect Dis. 2006 Apr 15;42 Suppl 4:S164-72
references: 16544267
references: Malays J Med Sci. 2007 Jul;14(2):36-40
references: 22993489
references: J Antimicrob Chemother. 2009 Jun;63(6):1286-8
references: 19297376
references: Indian J Med Microbiol. 2006 Jul;24(3):205-7
references: 16912441
references: J Infect. 2006 Oct;53(4):279-88
references: 16488476
references: J Antimicrob Chemother. 2010 Mar;65(3):490-5
references: 20071363
references: J Biomed Biotechnol. 2009;2009:165637
references: 19672454
references: J Antimicrob Chemother. 2011 Apr;66(4):745-51
references: 21393132
references: Antimicrob Agents Chemother. 2004 Jan;48(1):1-14
references: 14693512
references: Int J Microbiol. 2011;2011:
references: 20885968
references: Clin Microbiol Rev. 2005 Oct;18(4):657-86
references: 16223952
references: J Med Microbiol. 2008 Oct;57(Pt 10):1273-1276
references: 18809557
references: Appl Environ Microbiol. 2010 Sep;76(17):5972-6
references: 20639374
references: Clin Microbiol Rev. 1995 Oct;8(4):557-84
references: 8665470
references: Antimicrob Agents Chemother. 2003 Jan;47(1):19-26
references: 12499163
references: Int J Antimicrob Agents. 2004 Oct;24(4):327-33
references: 15380256
references: J Infect Dis. 1996 Sep;174(3):529-36
references: 8769610