title: Organization and regulation of the arsenite oxidase operon of the moderately acidophilic and facultative chemoautotrophic Thiomonas arsenitoxydans.
abstract: Thiomonas arsenitoxydans is an acidophilic and facultatively autotrophic bacterium that can grow by oxidizing arsenite to arsenate. A comparative genomic analysis showed that the T. arsenitoxydans aioBA cluster encoding the two subunits of arsenite oxidase is distinct from the other clusters, with two specific genes encoding a cytochrome c and a metalloregulator belonging to the ArsR/SmtB family. These genes are cotranscribed with aioBA, suggesting that these cytochromes c are involved in arsenite oxidation and that this operon is controlled by the metalloregulator. The growth of T. arsenitoxydans in the presence of thiosulfate and arsenite, or arsenate, is biphasic. Real-time PCR experiments showed that the operon is transcribed during the second growth phase in the presence of arsenite or arsenate, whereas antimonite had no effect. These results suggest that the expression of the aioBA operon of T. arsenitoxydans is regulated by the electron donor present in the medium, i.e., is induced in the presence of arsenic but is repressed by more energetic substrates. Our data indicate that the genetic organization and regulation of the aioBA operon of T. arsenitoxydans differ from those of the other arsenite oxidizers.
authors: Aix-Marseille Université, CNRS, LCB UMR7283, 13009, Marseille, France.
authors: Djamila
authors: D
authors: Slyemi
authors: Danielle
authors: D
authors: Moinier
authors: Emmanuel
authors: E
authors: Talla
authors: Violaine
authors: V
authors: Bonnefoy
mesh_terms: D000965:Antimony / Q000378:metabolism; D001149:Arsenates / Q000378:metabolism; D001151:Arsenic / Q000378:metabolism; D018053:Arsenites / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D020562:Betaproteobacteria / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D009876:Operon; D010088:Oxidoreductases / Q000235:genetics* / Q000378:metabolism
chemical_list: D001149:Arsenates; D018053:Arsenites; D001426:Bacterial Proteins; C061374:antimonite; D000965:Antimony; D010088:Oxidoreductases; C078179:arsenite oxidase; D001151:Arsenic
references: Nucleic Acids Res. 2013 Jan;41(Database issue):D636-47
references: 23193269
references: BMC Genomics. 2009 Aug 24;10:394
references: 19703284
references: Environ Microbiol. 2012 Dec;14(12):3097-109
references: 23057575
references: J Bacteriol. 2006 Feb;188(3):1081-8
references: 16428412
references: Nucleic Acids Res. 2012 Jan;40(Database issue):D130-5
references: 22121212
references: Microbiology (Reading). 1997 Jul;143 ( Pt 7):2179-2187
references: 9245807
references: J Bacteriol. 2005 Jun;187(12):4214-21
references: 15937183
references: J Bacteriol. 2012 Nov;194(22):6355
references: 23105084
references: Arch Microbiol. 2011 Jun;193(6):439-49
references: 21409355
references: J Biol Chem. 2007 Nov 2;282(44):32298-310
references: 17726022
references: PLoS Genet. 2007 Apr 13;3(4):e53
references: 17432936
references: J Biol Chem. 1992 Nov 25;267(33):23674-82
references: 1331097
references: PLoS Genet. 2010 Feb 26;6(2):e1000859
references: 20195515
references: J Biol Chem. 2010 Jul 2;285(27):20433-41
references: 20421652
references: Appl Environ Microbiol. 2005 Feb;71(2):599-608
references: 15691908
references: J Mol Biol. 1990 Oct 5;215(3):403-10
references: 2231712
references: Appl Environ Microbiol. 2010 Oct;76(20):6804-11
references: 20729322
references: Appl Environ Microbiol. 2004 Jun;70(6):3383-91
references: 15184135
references: Bioinformatics. 2007 Nov 1;23(21):2947-8
references: 17846036
references: Nat Protoc. 2009;4(3):363-71
references: 19247286
references: FEMS Microbiol Lett. 2010 Dec;313(1):20-8
references: 21039781
references: Biomol NMR Assign. 2013 Oct;7(2):145-8
references: 22618866
references: J Bacteriol. 2012 Dec;194(24):6986-7
references: 23209236
references: Int J Syst Evol Microbiol. 2007 Mar;57(Pt 3):504-512
references: 17329775
references: Microbiology (Reading). 2005 May;151(Pt 5):1707-1716
references: 15870478
references: Environ Microbiol Rep. 2012 Dec;4(6):571-86
references: 23760928
references: BMC Genomics. 2010 Dec 17;11:709
references: 21167028
references: Biochemistry. 2012 Mar 27;51(12):2619-29
references: 22394357
references: J Biol Chem. 1993 Jan 5;268(1):52-8
references: 8416957
references: Appl Environ Microbiol. 2009 Aug;75(15):5141-7
references: 19525272
references: Genome Biol Evol. 2013;5(5):934-53
references: 23589360
references: Environ Microbiol. 2008 Jan;10(1):228-37
references: 17894815
references: Microbiology (Reading). 2007 Jan;153(Pt 1):80-91
references: 17185537
references: Chem Rev. 2009 Oct;109(10):4644-81
references: 19788177
references: J Bacteriol. 2012 Mar;194(5):1243-4
references: 22328747
references: Biochim Biophys Acta. 2004 Jun 7;1656(2-3):148-55
references: 15178476
references: BMC Microbiol. 2010 Feb 18;10:53
references: 20167112
references: J Bacteriol. 2012 Feb;194(4):903
references: 22275101
references: Biochim Biophys Acta. 2007 Feb;1767(2):189-96
references: 17306216
references: J Biol Chem. 2011 Jul 22;286(29):26148-57
references: 21632538
references: Environ Microbiol. 2012 Jul;14(7):1624-34
references: 22176720
references: J Bacteriol. 2012 Mar;194(6):1635-6
references: 22374962
references: Environ Microbiol. 2006 May;8(5):899-908
references: 16623746
references: J Bacteriol. 2012 Sep;194(18):5153
references: 22933773
references: Appl Microbiol Biotechnol. 2009 Jun;83(4):715-25
references: 19283378
references: Nat Prod Rep. 2010 May;27(5):668-80
references: 20442958
references: Appl Environ Microbiol. 2012 Aug;78(16):5638-45
references: 22660703