title: Oxygen biosensor based on bilirubin oxidase immobilized on a nanostructured gold electrode.
abstract: Gold disk electrodes modified with gold nanoparticles have been used as a scaffold for the covalent immobilization of bilirubin oxidase. The nanostructured bioelectrodes were tested as mediator-less biosensors for oxygen in a buffer that mimics the content and the composition of human physiological fluids. Chronoamperometry measurements showed a detection limit towards oxygen of 6 ± 1 μM with a linear range of 6-300 μM, i.e. exceeding usual physiological ranges of oxygen in human tissues and fluids. The biosensor presented is the first ever-reported oxygen amperometric biosensor based on direct electron transfer of bilirubin oxidase.
authors: Instituto de Catalisis y Petroleoquimica, Consejo Superior de Investigaciones Cientificas, C/Marie Curie 2, L10, 28049 Madrid, Spain. Electronic address: marcospita@icp.csic.es.
authors: Marcos
authors: M
authors: Pita
authors: Cristina
authors: C
authors: Gutierrez-Sanchez
authors: Miguel D
authors: MD
authors: Toscano
authors: Sergey
authors: S
authors: Shleev
authors: Antonio L
authors: AL
authors: De Lacey
mesh_terms: D015374:Biosensing Techniques; D004566:Electrodes; D006046:Gold / Q000737:chemistry; D006801:Humans; D055767:Immobilized Proteins / Q000737:chemistry*; D049329:Nanostructures / Q000737:chemistry; D044925:Oxidoreductases Acting on CH-CH Group Donors / Q000737:chemistry*; D010100:Oxygen / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D055767:Immobilized Proteins; D006046:Gold; D044925:Oxidoreductases Acting on CH-CH Group Donors; C042401:bilirubin oxidase; D010100:Oxygen
keywords: Bilirubin oxidase; Biocatalysis; Biosensor; Direct electron transfer; Gold nanoparticles