title: Increased proliferation and differentiation of pre-osteoblasts MC3T3-E1 cells on nanostructured polypyrrole membrane under combined electrical and mechanical stimulation.
abstract: Polypyrrole (PPy), as an electrical conductive polymer, has been widely investigated in biomedical fields. In this study, PPy membrane at nanoscale was electrically deposited on indium-tin oxide glass slide with sodium p-toluenesulfonate as supporting electrolyte. Electropolymerization of PPy was performed under a constant 800 mV voltage for 10 seconds. Chemical compositions and morphology were characterized by Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM). The results showed that the nanoscaled PPy particles distributed uniformly and the average diameter of PPy particles was 62 nm. Since bone cells can respond to both electrical and mechanical stimulation in vivo, pre-osteoblasts MC3T3-E1 cells were cultured ort nanostructured PPy membrane under the combined electrical and mechanical stimulation. The nano-PPy membrane was conducive to transferring uniform electrical stimulation and applying steady mechanical stimulation. It is suggested that the combined stimulation did not affect cells morphologies significantly. However, cell proliferation tested by MTT, alkaline phosphatase activities, and gene expression of Collagen-I indicated that combined stimulation can enhance the proliferation and differentiation of MC3T3-E1 cells more efficiently than single electrical stimulation or single mechanical stimulation. The combined stimulation through a nano-PPy membrane may provide a highly potential stimulated method in bone tissue engineering.
authors: Key Laboratory for Biomechanics and Mechanobiology of the Ministry of Education, School of Biological Science and Medical Engineering, Beihang University, Beijing 100191, China.
authors: Lizhen
authors: L
authors: Liu
authors: Ping
authors: P
authors: Li
authors: Gang
authors: G
authors: Zhou
authors: Menghang
authors: M
authors: Wang
authors: Xiaoling
authors: X
authors: Jia
authors: Meili
authors: M
authors: Liu
authors: Xufeng
authors: X
authors: Niu
authors: Wei
authors: W
authors: Song
authors: Haifeng
authors: H
authors: Liu
authors: Yubo
authors: Y
authors: Fan
mesh_terms: D000818:Animals; D041702:BALB 3T3 Cells; D001672:Biocompatible Materials / Q000138:chemical synthesis; D002454:Cell Differentiation / Q000502:physiology; D049109:Cell Proliferation; D004558:Electric Stimulation / Q000379:methods; D008422:Materials Testing; D040542:Mechanotransduction, Cellular / Q000502:physiology*; D008567:Membranes, Artificial; D051379:Mice; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D010006:Osteoblasts / Q000166:cytology* / Q000502:physiology*; D010012:Osteogenesis / Q000502:physiology*; D010812:Physical Stimulation / Q000379:methods; D011108:Polymers / Q000737:chemistry*; D011758:Pyrroles / Q000737:chemistry*; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D001672:Biocompatible Materials; D008567:Membranes, Artificial; D011108:Polymers; D011758:Pyrroles; C067635:polypyrrole