title: Involvement of mutation in ampD I, mrcA, and at least one additional gene in β-lactamase hyperproduction in Stenotrophomonas maltophilia.
abstract: It has been reported that targeted disruption of ampD I or mrcA causes β-lactamase hyperproduction in Stenotrophomonas maltophilia. We show here that β-lactamase-hyperproducing laboratory selected mutants and clinical isolates can have wild-type ampD I and mrcA genes, implicating mutation of at least one additional gene in this phenotype. The involvement of mutations at multiple loci in the activation of β-lactamase production in S. maltophilia reveals that there are significant deviations from the enterobacterial paradigm of AmpR-mediated control of β-lactamase induction. We do show, however, that S. maltophilia ampD I can complement a mutation in Escherichia coli ampD. This suggests that an anhydromuropeptide degradation product of peptidoglycan is used to activate AmpR in S. maltophilia, as is also the case in enteric bacteria.
authors: School of Cellular and Molecular Medicine, University of Bristol, Bristol, United Kingdom.
authors: Asmaa
authors: A
authors: Talfan
authors: Oliver
authors: O
authors: Mounsey
authors: Matthew
authors: M
authors: Charman
authors: Eleanor
authors: E
authors: Townsend
authors: Matthew B
authors: MB
authors: Avison
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D005816:Genetic Complementation Test; D005838:Genotype; D006801:Humans; D009154:Mutation; D009238:N-Acetylmuramoyl-L-alanine Amidase / Q000235:genetics* / Q000378:metabolism; D010457:Peptidoglycan / Q000378:metabolism; D010641:Phenotype; D059748:Proteolysis; D020615:Stenotrophomonas maltophilia / Q000235:genetics* / Q000378:metabolism; D001618:beta-Lactamases / Q000096:biosynthesis / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D010457:Peptidoglycan; C071526:AmpR protein, Bacteria; C062460:AmpD protein, Bacteria; D009238:N-Acetylmuramoyl-L-alanine Amidase; D001618:beta-Lactamases
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