title: Therapeutic Effect of the Tuber of Alisma orientale on Lipopolysaccharide-Induced Acute Lung Injury.
abstract: Although Alisma orientale, an ethnic herb, has been prescribed for treating various diseases in Asian traditional medicine, experimental evidence to support its therapeutic effects is lacking. Here, we sought to determine whether A. orientale has a therapeutic effect on acute lung injury (ALI). Ethanol extract of the tuber of A. orientale (EEAO) was prepared and fingerprinted by HPLC for its constituents. Mice received an intraperitoneal (i.p.) injection of lipopolysaccharide (LPS) for the induction of ALI. At 2 h after LPS treatment, mice received an intratracheal (i.t.) spraying of various amounts of EEAO to the lung. Bioluminescence imaging of transgenic NF- κ B/luciferase reporter mice shows that i.t. EEAO posttreatment suppressed lung inflammation. In similar experiments with C57BL/6 mice, EEAO posttreatment significantly improved lung inflammation, as assessed by H&E staining of lung sections, counting of neutrophils in bronchoalveolar lavage fluid, and semiquantitative RT-PCR analyses of proinflammatory cytokines and Nrf2-dependent genes in the inflamed lungs. Furthermore, EEAO posttreatment enhanced the survival of mice that received a lethal dose of LPS. Together, our results provide evidence that A. orientale has a therapeutic effect on ALI induced by sepsis.
authors: School of Korean Medicine, Pusan National University, Yangsan 626-870, Republic of Korea.
authors: Kyun Ha
authors: KH
authors: Kim
authors: Min Jung
authors: MJ
authors: Kwun
authors: Jun-Yong
authors: JY
authors: Choi
authors: Kyung-Seop
authors: KS
authors: Ahn
authors: Sei-Ryang
authors: SR
authors: Oh
authors: Yong Gyu
authors: YG
authors: Lee
authors: John W
authors: JW
authors: Christman
authors: Ruxana T
authors: RT
authors: Sadikot
authors: Chang Woo
authors: CW
authors: Han
authors: Myungsoo
authors: M
authors: Joo
references: Biol Pharm Bull. 1998 Dec;21(12):1317-21
references: 9881646
references: Ann N Y Acad Sci. 2008 Dec;1147:61-9
references: 19076431
references: Bioorg Med Chem Lett. 1999 Nov 1;9(21):3081-6
references: 10560729
references: Crit Care Clin. 2011 Jul;27(3):439-58
references: 21742210
references: Int J Exp Pathol. 2007 Dec;88(6):387-91
references: 18039275
references: J Aerosol Med. 1994;7(1):49-75
references: 10147058
references: Chest. 2007 Mar;131(3):913-920
references: 17356114
references: J Pathol. 2004 Feb;202(2):145-56
references: 14743496
references: J Biol Chem. 2009 May 15;284(20):13291-5
references: 19182219
references: Trends Mol Med. 2011 Jul;17(7):363-71
references: 21459041
references: Planta Med. 2002 Mar;68(3):226-31
references: 11914959
references: J Clin Invest. 2004 Nov;114(9):1248-59
references: 15520857
references: Inflammation. 2005 Apr;29(2-3):72-80
references: 16858644
references: Proc Natl Acad Sci U S A. 2009 Jan 6;106(1):250-5
references: 19104057
references: Arch Pharm Res. 2012 Nov;35(11):1919-26
references: 23212633
references: J Biol Chem. 2010 Mar 26;285(13):9932-9948
references: 20054000
references: J Immunol. 2006 Apr 15;176(8):4923-30
references: 16585588
references: J Clin Invest. 2006 Apr;116(4):984-95
references: 16585964
references: J Exp Med. 2005 Jul 4;202(1):47-59
references: 15998787
references: J Ethnopharmacol. 2013 Mar 7;146(1):402-10
references: 23333748
references: N Engl J Med. 2000 May 4;342(18):1334-49
references: 10793167
references: Am Rev Respir Dis. 1986 May;133(5):913-27
references: 3085564
references: Planta Med. 2006 Aug;72(10):951-4
references: 16858666
references: Zhonghua Yi Xue Za Zhi. 2004 Aug 2;84(15):1276-9
references: 15387965
references: Proc Natl Acad Sci U S A. 1999 Oct 26;96(22):12731-6
references: 10535991
references: Am J Respir Crit Care Med. 2003 Aug 1;168(3):287-96
references: 12773330
references: Biochem Pharmacol. 2008 Dec 1;76(11):1485-9
references: 18694732
references: Shock. 1996;6 Suppl 1:S23-6
references: 8828094
references: Am J Respir Crit Care Med. 2003 Apr 1;167(7):1027-35
references: 12663342
references: Zhongguo Zhong Yao Za Zhi. 1991 Oct;16(10):622-5, inside back cover
references: 1820797
references: Chest. 2002 Dec;122(6 Suppl):314S-320S
references: 12475808
references: N Engl J Med. 2005 Oct 20;353(16):1685-93
references: 16236739
references: Biol Pharm Bull. 2012;35(9):1581-7
references: 22975512
references: Zhongguo Zhong Yao Za Zhi. 2003 Nov;28(11):1072-5
references: 15615421
references: Biol Pharm Bull. 1997 May;20(5):511-6
references: 9178931
references: Mol Cell Biol. 2007 Sep;27(18):6334-49
references: 17636022
references: Arch Pharm Res. 2004 Apr;27(4):460-4
references: 15180314
references: Am J Physiol Lung Cell Mol Physiol. 2009 Mar;296(3):L320-7
references: 19098124
references: J Pharm Pharmacol. 2006 Oct;58(10):1391-8
references: 17034663
references: Pharm Biol. 2012 Oct;50(10):1281-8
references: 22857151
references: Crit Care Clin. 2000 Apr;16(2):289-317
references: 10768083