title: Immunodiagnosis of fascioliasis using sandwich enzyme-linked immunosorbent assay for detection of Fasciola gigantica paramyosin antigen.
abstract: BACKGROUND
  Many immunological techniques have been developed over years using the different Fasciola antigens for diagnosis of parasitic infection and to replace the parasitological techniques, which are time consuming and usually lack sensitivity and reproducibility.
  
  
  METHODS
  In this study, Fasciola gigantica paramyosin (Pmy) antigen was early detected in cattle sera using sandwich enzyme-linked immunosorbent assay (ELISA), to evaluate the Pmy antigen performance in diagnosis. This work was conducted on 135 cattle blood samples, which were classified according to parasitological investigation into, healthy control (30), fascioliasis (75), and other parasites (30) groups.
  
  
  RESULTS
  The sensitivity of Sandwich ELISA was 97.33%, and the specificity was 95%, in comparison with parasitological examination, which recorded 66.66% sensitivity and 100% specificity, respectively.
  
  
  CONCLUSIONS
  It was clear that the native F. gigantica Pmy is considered as a powerful antigen in early immunodiagnosis of fascioliasis, using a highly sensitive and specific sandwich ELISA technique.
authors: Department of Zoology, Faculty of Science, Cairo University, Giza, Egypt.
authors: Hany Mohamed Adel
authors: HM
authors: Abou-Elhakam
authors: Ibraheem Rabia
authors: IR
authors: Bauomy
authors: Somaya Osman
authors: SO
authors: El Deeb
authors: Azza Mohamed
authors: AM
authors: El Amir
keywords: Enzyme-linked immunosorbent assay; Fasciola gigantica; paramyosin; polyclonal antibodies; sensitivity; specificity
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