title: Qindan capsule changes adventitial collagen synthesis in spontaneously hypertensive rats.
abstract: OBJECTIVE
  To investigate the effect of Qindan capsule (QC) on collagen synthesis and the mechanism underlying the process in spontaneously hypertensive rats (SHRs).
  
  
  METHODS
  Twentyfour SHRs were divided into three groups: the hypertension model group, the QC treatment group, and the losartan treatment group. Eight Wistar Kyoto (WKY) rats were used as the normal control group. The systolic blood pressure (SBP) of the rats was monitored, and the thoracic aorta adventitia of the rats was segregated. The expressions of transforming growth factor 1 (TGF-β1), Smad3, and collagens I and were measured by histological staining and reverse transcription polymerase chain reaction.
  
  
  RESULTS
  The SBP was significantly higher in the model group than in the normal control group (P<0.01). However, a significant SBP-lowering effect was observed in QC or losartan treatment groups (P<0.05 or P<0.01) after 3 weeks of treatment. QC-treated rats showed a decrease of approximately 40 mm Hg, and the losartan-treated rats showed a decrease of approximately 50 mm Hg at the end of treatment compared with the beginning of treatment. The protein and gene levels of TGF-β1, Smad3, and collagens I and in the model group were significantly increased compared with those in the normal control group (P<0.01). However, the levels were significantly decreased in the QC or losartan treatment group compared with the model group (P<0.05 or P<0.01). However, there was no significant difference between the QC and losartan treatment groups (P<0.05).
  
  
  CONCLUSIONS
  QC could exert its antihypertensive effect through down-regulating TGF-β1-stimulated collagen expressions. The TGF-β1/Smad3 signaling pathway may be involved in this process.
authors: Department of Traditional Chinese Medicine, Qilu Hospital, Shandong University, Jinan, China.
authors: Yi-jing
authors: YJ
authors: Lv
authors: Gui-lin
authors: GL
authors: Liu
authors: Xu-ming
authors: XM
authors: Ji
authors: Cun-zhong
authors: CZ
authors: Yuan
authors: Bo
authors: B
authors: Wang
authors: Min
authors: M
authors: Ren
authors: Lei
authors: L
authors: Yan
authors: Xu-ping
authors: XP
authors: Wang
authors: Ji-dong
authors: JD
authors: Zhang
mesh_terms: D063194:Adventitia / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D000818:Animals; D001794:Blood Pressure / Q000187:drug effects; D001808:Blood Vessels / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D002214:Capsules; D003094:Collagen / Q000096:biosynthesis*; D024042:Collagen Type I / Q000235:genetics / Q000378:metabolism; D024061:Collagen Type III / Q000235:genetics / Q000378:metabolism; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D019808:Losartan / Q000494:pharmacology; D008297:Male; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY; D051900:Smad3 Protein / Q000235:genetics / Q000378:metabolism; D013194:Staining and Labeling; D013599:Systole / Q000187:drug effects; D053773:Transforming Growth Factor beta1 / Q000235:genetics / Q000378:metabolism
chemical_list: D002214:Capsules; D024042:Collagen Type I; D024061:Collagen Type III; D004365:Drugs, Chinese Herbal; D012333:RNA, Messenger; D051900:Smad3 Protein; D053773:Transforming Growth Factor beta1; C526717:qindan; D003094:Collagen; D019808:Losartan
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