title: Quantitative determination of neurotransmitters, metabolites and derivates in microdialysates by UHPLC-tandem mass spectrometry.
abstract: The main objective of the present work is to study the time-course of rat brain neurotransmitters in vivo after an oral challenge with a nutritional ingredient or an external stimulus, such as a chemical agent. An ultrahigh performance liquid chromatography-tandem mass spectrometry method for the identification and quantification of neurotransmitters, metabolites and derivates in microdialysates from rat brain was previously developed. Betaine, glutamine, glutamic acid, gamma-aminobutyric acid, phosphocholine, glycerophosphocholine, cytidine 5'-diphosphocholine, choline, acetylcholine, dopamine, norepinephrine, serotonin, tyrosine, epinephrine, tryptophan and 5-hydroxyindoleacetic acid were selected as analytes. The method involves direct injection of samples of microdialysates from rat brain onto the chromatographic equipment and quantification with a triple quadrupole mass spectrometer detector using an electrospray ionization interface in positive mode. The limits of detection ranged from 0.1 to 50 ng mL(-1) and the limits of quantification from 0.3 to 200 ng mL(-1). The inter- and intra-day variability were lower than 15%. Recovery rates ranged from 85% to 115%.
authors: Discovery R&D, Abbott Nutrition, Abbott Laboratories, Cmno. Purchil 68, E-18004 Granada, Spain. angela.santos@abbott.com
authors: A
authors: A
authors: Santos-Fandila
authors: A
authors: A
authors: Zafra-Gómez
authors: A
authors: A
authors: Barranco
authors: A
authors: A
authors: Navalón
authors: R
authors: R
authors: Rueda
authors: M
authors: M
authors: Ramírez
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005947:Glucose / Q000494:pharmacology; D017551:Microdialysis; D018377:Neurotransmitter Agents / Q000032:analysis* / Q000378:metabolism; D011189:Potassium Chloride / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D053719:Tandem Mass Spectrometry
chemical_list: D018377:Neurotransmitter Agents; D011189:Potassium Chloride; D005947:Glucose
keywords: Electrospray ionization; Microdialysis; Neurotransmitters; UHPLC–MS/MS