title: Saccharopolyspora cavernae sp. nov., a novel actinomycete isolated from the Swallow Cave in Yunnan, south-west China.
abstract: A Gram-positive, aerobic, non-motile actinobacterial strain, designated YIM C01235(T), was isolated from a soil sample collected from the Swallow Cave, Yunnan province, south-west China. The isolate grew at 10-30 °C, pH 6.0-9.0 and 0-8 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences revealed highest similarity to Saccharopolyspora gloriosae YIM 60513(T) (96.8 %), and lower 16S rRNA gene sequence similarities (95.1-96.7 %) with the other species of the genus Saccharopolyspora. The whole-cell hydrolysates contained meso-diaminopimelic acid (meso-DAP), arabinose and galactose. The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylmethylethanolamine, two unknown glycolipids, two unknown phospholipids and one polar lipid. MK-9(H4) was the predominant menaquinone. The major fatty acids were iso-C15:0, iso-C16:0, anteiso-C15:0 and anteiso-C17:0. Mycolic acids were not detected. The genomic DNA G+C content was 69.1 mol%. These chemotaxonomic data, together with its morphological properties, were consistent with the assignment of strain YIM C01235(T) to the genus Saccharopolyspora. The results of physiological and biochemical tests allowed strain YIM C01235(T) to be differentiated phenotypically from all the recognized Saccharopolyspora species. On the basis of evidence from this polyphasic study, the novel species Saccharopolyspora cavernae sp. nov. is proposed. The type strain is YIM C01235(T) (=DSM 45825(T) = CCTCC AA 2012022(T)).
authors: Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education, and Laboratory for Conservation and Utilization of Bio-resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, 650091, People's Republic of China.
authors: Juan
authors: J
authors: Cheng
authors: Yong-Guang
authors: YG
authors: Zhang
authors: Wei
authors: W
authors: Chen
authors: Li
authors: L
authors: Li
authors: Dao-Feng
authors: DF
authors: Zhang
authors: Hong-Fei
authors: HF
authors: Wang
authors: Xin-Hua
authors: XH
authors: Lu
authors: Yan-Qing
authors: YQ
authors: Duan
authors: Wen-Jun
authors: WJ
authors: Li
mesh_terms: D000332:Aerobiosis; D000596:Amino Acids / Q000032:analysis; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002241:Carbohydrates / Q000032:analysis; D002244:Carbon / Q000378:metabolism; D002681:China; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D009584:Nitrogen / Q000378:metabolism; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D011809:Quinones / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D016930:Saccharopolyspora / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride / Q000378:metabolism; D012988:Soil Microbiology; D013696:Temperature
chemical_list: D000596:Amino Acids; D002241:Carbohydrates; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D010743:Phospholipids; D011809:Quinones; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride; D002244:Carbon; D009584:Nitrogen