title: Analysis of mRNA expression profiles of carotenogenesis and astaxanthin production of Haematococcus pluvialis under exogenous 2, 4-epibrassinolide (EBR).
abstract: The fresh-water green unicellular alga Haematococcus pluvialis is known to accumulate astaxanthin under stress conditions. In the present study, transcriptional expression of eight genes involved in astaxanthin biosynthesis exposed to EBR (25 and 50 mg/L) was analyzed using qRT-PCR. The results demonstrated that both 25 and 50 mg/L EBR could increase astaxanthin productivity and the eight carotenogenic genes were up-regulated by EBR with different expression profiles. Moreover, EBR25 induction had a greater influence on the transcriptional expression of ipi-1, ipi-2, crtR-B, lyc and crtO (> 5- fold up-regulation) than on psy, pds, bkt; EBR50 treatment had a greater effect on the transcriptional expression of ipi-2, pds, lyc, crtR-B, bkt and crtO than on ipi-1 and psy. Furthermore, astaxanthin biosynthesis under EBR was up-regulated mainly by ipi1־ and psy at the post-transcriptional level, pds, lyc, crtR-B, bkt and crtO at the transcriptional level and ipi-2 at both levels.
authors: School of Life Sciences, Shandong University of Technology, Zibo, China, 255049.
authors: Zhengquan
authors: Z
authors: Gao
authors: Chunxiao
authors: C
authors: Meng
authors: Hongzheng
authors: H
authors: Gao
authors: Xiaowen
authors: X
authors: Zhang
authors: Dong
authors: D
authors: Xu
authors: Yuanfeng
authors: Y
authors: Su
authors: Yuanyuan
authors: Y
authors: Wang
authors: Yuren
authors: Y
authors: Zhao
authors: Naihao
authors: N
authors: Ye
mesh_terms: D000704:Analysis of Variance; D060406:Brassinosteroids / Q000494:pharmacology*; D002338:Carotenoids / Q000096:biosynthesis* / Q000235:genetics; D000460:Chlorophyta / Q000166:cytology / Q000235:genetics*; D010937:Plant Growth Regulators / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction; D013260:Steroids, Heterocyclic / Q000494:pharmacology*; D014158:Transcription, Genetic; D024341:Xanthophylls / Q000096:biosynthesis
chemical_list: D060406:Brassinosteroids; D010937:Plant Growth Regulators; D012333:RNA, Messenger; D013260:Steroids, Heterocyclic; D024341:Xanthophylls; D002338:Carotenoids; C005948:astaxanthine; C023623:brassinolide