title: The association of IFI27 expression and fatigue intensification during localized radiation therapy: implication of a para-inflammatory bystander response.
abstract: The mechanisms behind fatigue intensification during cancer therapy remain elusive. The interferon alpha-inducible protein 27 (IFI27) was the most up-regulated gene based on our previous microarray data in fatigued men with non-metastatic prostate cancer receiving localized external beam radiation therapy (EBRT). The purpose of this study was to confirm the IFI27 up-regulation and determine its association with fatigue intensification during EBRT. Peripheral blood samples and fatigue scores were collected at three time points--prior to EBRT, at midpoint, and at completion of EBRT. Confirmatory quantitative real time polymerase chain reaction (qPCR) and enzyme-linked immunosorbent assay (ELISA) were utilized to verify the microarray results. Subjects were a total of 40 Caucasian men with prostate cancer; 20 scheduled for EBRT (65.6 ± 7.5 years old), and 20 on active surveillance as controls (62.8 ± 6.1 years old). Significant IFI27 expression overtime during EBRT was confirmed by qPCR (p < 0.5), which correlated with fatigue scores during EBRT (R = -0.90, p = 0.006). Alterations in mechanisms associated with immune response and mitochondrial function that explain the up-regulation of IFI27 may provide an understanding of the pathways related to the intensification of fatigue during localized radiation therapy.
authors: Frances Payne Bolton School of Nursing, Case Western Reserve University, 2120 Cornell Road, Cleveland, OH 44106, USA.
authors: Chao-Pin
authors: CP
authors: Hsiao
authors: Maria
authors: M
authors: Araneta
authors: Xiao Min
authors: XM
authors: Wang
authors: Leorey N
authors: LN
authors: Saligan
mesh_terms: D000368:Aged; D024201:Bystander Effect / Q000528:radiation effects; D016022:Case-Control Studies; D005221:Fatigue / Q000235:genetics*; D006801:Humans; D018836:Inflammation Mediators / Q000378:metabolism; D008297:Male; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis; D011471:Prostatic Neoplasms / Q000235:genetics / Q000532:radiotherapy*; D011832:Radiation Injuries / Q000235:genetics*; D059467:Transcriptome / Q000528:radiation effects
chemical_list: C406887:IFI27 protein, human; D018836:Inflammation Mediators; D008565:Membrane Proteins
references: Inflammopharmacology. 2009 Oct;17(5):255-66
references: 19798548
references: Oncol Nurs Forum. 1998 May;25(4):677-84
references: 9599351
references: Lancet. 2008 Jul 12;372(9633):117-26
references: 18620949
references: Mutat Res. 2010 May 1;687(1-2):28-33
references: 20080112
references: J Pain Symptom Manage. 2004 Oct;28(4):373-80
references: 15471655
references: J Pain Symptom Manage. 2008 Jun;35(6):632-43
references: 18358683
references: J Cancer Res Ther. 2010 Oct-Dec;6(4):516-20
references: 21358092
references: Clin Cancer Res. 2009 Sep 1;15(17):5534-40
references: 19706826
references: Qual Life Res. 2009 Sep;18(7):873-80
references: 19543809
references: Mutat Res. 2011 Aug 1;713(1-2):32-8
references: 21645526
references: Psychosom Med. 2002 Jul-Aug;64(4):604-11
references: 12140350
references: Cancer Nurs. 2013 May-Jun;36(3):189-97
references: 23047795
references: Brain Behav Immun. 2012 Aug;26(6):830-48
references: 22595751
references: Clin Exp Immunol. 2005 Dec;142(3):505-11
references: 16297163
references: Brain Behav Immun. 2011 Aug;25(6):1094-8
references: 21356304
references: Br J Cancer. 1998 Oct;78(7):899-906
references: 9764581
references: Nature. 2008 Jul 24;454(7203):428-35
references: 18650913
references: Brain Behav Immun. 2007 Mar;21(3):251-8
references: 17008048
references: J Psychosom Res. 2004 Feb;56(2):157-70
references: 15016573
references: EMBO J. 1986 Jul;5(7):1601-6
references: 3017706
references: Brain Behav Immun. 2012 Feb;26(2):218-27
references: 21958477
references: Brain Behav Immun. 2012 Jul;26(5):706-13
references: 22406004
references: Pain. 2007 Mar;128(1-2):136-47
references: 17070997
references: Nat Immunol. 2001 Jul;2(7):585-9
references: 11429541
references: Arch Otolaryngol Head Neck Surg. 2008 May;134(5):528-35
references: 18490576
references: Cancer Res. 2011 Apr 1;71(7):2488-96
references: 21300764
references: J Cancer. 2011 Feb 10;2:81-9
references: 21326629
references: J Clin Epidemiol. 2011 May;64(5):507-16
references: 21447427
references: Cancer. 2009 Dec 1;115(23):5596-606
references: 19670452
references: Biol Res Nurs. 2009 Jul;11(1):27-41
references: 19419979
references: Apoptosis. 2008 Apr;13(4):562-72
references: 18330707
references: Clin Cancer Res. 2006 May 1;12(9):2759-66
references: 16675568
references: Biol Res Nurs. 2006 Oct;8(2):157-69
references: 17003255
references: Int J Radiat Biol. 2012 Oct;88(10):751-62
references: 22709338
references: Trends Immunol. 2002 Apr;23(4):201-8
references: 11923115
references: Brain Behav Immun. 2013 Jan;27(1):63-70
references: 23022913
references: Clin Sci (Lond). 2004 Sep;107(3):281-9
references: 15128285
references: Int J Radiat Oncol Biol Phys. 2004 May 1;59(1):160-7
references: 15093912
references: J Clin Oncol. 2011 Sep 10;29(26):3517-22
references: 21825266
references: Oncol Nurs Forum. 2006 Jan 01;33(1):E18-26
references: 16470230
references: Cancer Res. 1993 Sep 1;53(17):4096-101
references: 8358738
references: Br J Cancer. 2012 Jan 17;106(2):284-9
references: 22215065