title: Prognostic significance of immunoglobulin M overexpression in laryngeal squamous cell carcinoma.
abstract: CONCLUSIONS
  Immunoglobulin (Ig) M is overexpressed in laryngeal squamous cell carcinoma (LSCC), and its expression has an independent protective impact on disease-free survival in LSCC.
  
  
  OBJECTIVE
  A number of studies have reported on the ectopic expression of Ig in cancer cells, yet there has been a lack of understanding of its clinical and prognostic significance. This study aimed to investigate the expression of IgM in resected specimens of LSCC and to evaluate its clinical significance and prognostic value.
  
  
  METHODS
  Immunohistochemistry (IHC) and Western blotting were used to detect the expression of IgM in LSCC and normal laryngeal tissues. The serum level of IgM was also analyzed by immunoturbidimetric assay.
  
  
  RESULTS
  IHC and Western blot studies demonstrated that IgM was overexpressed in LSCC specimens (p < 0.001), while the serum level of IgM in patients with LSCC was not different from healthy controls. Chi-squared analysis revealed that the expression level of IgM was negatively correlated with regional lymph node metastasis and tumor stage (p = 0.011 and 0.025, respectively). Univariate analysis showed that IgM expression was significantly correlated with enhanced disease-free survival (DFS) (p = 0.004). In multivariate analysis, IgM retained its independent prognostic value for DFS (p = 0.048, HR = 0.506, 95% CI = 0.257-0.995).
authors: Department of Otolaryngology Head and Neck Surgery, Affiliated Hospital of Luzhou Medical College , Luzhou, P.R. China.
authors: Haiyang
authors: H
authors: Wang
authors: Xu
authors: X
authors: Cao
authors: Eric-Chen
authors: EC
authors: Liu
authors: Dan
authors: D
authors: He
authors: Ying
authors: Y
authors: Ma
authors: Ting
authors: T
authors: Zhang
authors: Yang
authors: Y
authors: Feng
authors: Gang
authors: G
authors: Qin
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015153:Blotting, Western; D002294:Carcinoma, Squamous Cell / Q000097:blood* / Q000401:mortality / Q000473:pathology; D002681:China / Q000453:epidemiology; D018572:Disease-Free Survival; D005260:Female; D006801:Humans; D007075:Immunoglobulin M / Q000096:biosynthesis* / Q000097:blood; D007150:Immunohistochemistry; D007822:Laryngeal Neoplasms / Q000097:blood* / Q000401:mortality / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009364:Neoplasm Recurrence, Local; D011379:Prognosis; D015996:Survival Rate / Q000639:trends
chemical_list: D007075:Immunoglobulin M