title: The dynamics of DNA damage repair and transcription.
abstract: Recent advances have led to several systems to study transcription from defined loci in living cells. It has now become possible to address long-standing questions regarding the interplay between the processes of DNA damage repair and transcription-two disparate processes that can occur on the same stretch of chromatin and which both lead to extensive chromatin change. Here we describe the development of a system to create enzymatically induced DNA double-strand breaks (DSBs) at a site of inducible transcription and methods to study the interplay between these processes.
authors: Department of Cancer Biology, Abramson Family Cancer Research Institute, Basser Research Center for BRCA1/2, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.
authors: Niraj M
authors: NM
authors: Shanbhag
authors: Roger A
authors: RA
authors: Greenberg
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D000970:Antineoplastic Agents / Q000494:pharmacology; D002460:Cell Line; D002843:Chromatin / Q000235:genetics; D042002:Chromatin Assembly and Disassembly / Q000502:physiology; D053903:DNA Breaks, Double-Stranded; D004260:DNA Repair / Q000235:genetics* / Q000502:physiology; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D004318:Doxycycline / Q000494:pharmacology; D006801:Humans; D007763:Lac Operon / Q000235:genetics; D007834:Lasers; D008856:Microscopy, Fluorescence / Q000379:methods*; D013629:Tamoxifen / Q000031:analogs & derivatives / Q000494:pharmacology; D014158:Transcription, Genetic / Q000235:genetics* / Q000502:physiology
chemical_list: D000900:Anti-Bacterial Agents; D000970:Antineoplastic Agents; D002843:Chromatin; D013629:Tamoxifen; C016601:afimoxifene; C035042:endodeoxyribonuclease FokI; D015252:Deoxyribonucleases, Type II Site-Specific; D004318:Doxycycline
references: Chromosome Res. 2007;15(6):787-97
references: 17874213
references: Nat Struct Mol Biol. 2012 Feb 12;19(3):276-82
references: 22343725
references: Nat Cell Biol. 2007 Jun;9(6):683-90
references: 17486112
references: Nature. 2007 Jun 7;447(7145):730-4
references: 17554310
references: Cell. 2004 Mar 5;116(5):683-98
references: 15006351
references: Nat Cell Biol. 2007 Jun;9(6):675-82
references: 17486118
references: Proc Natl Acad Sci U S A. 1998 Sep 1;95(18):10564-9
references: 9724743
references: Mol Cell Biol. 1994 Dec;14(12):8096-106
references: 7969147
references: Proc Natl Acad Sci U S A. 1998 Sep 1;95(18):10570-5
references: 9724744
references: Cell. 2004 Sep 17;118(6):699-713
references: 15369670
references: Nat Cell Biol. 2003 Jun;5(6):572-7
references: 12766777
references: EMBO J. 2010 Apr 21;29(8):1446-57
references: 20360682
references: Mol Cell. 2007 Nov 9;28(3):386-97
references: 17996703
references: Cell. 2010 Jun 11;141(6):970-81
references: 20550933
references: Trends Biochem Sci. 2007 Apr;32(4):165-71
references: 17349792
references: Methods Mol Biol. 2011;714:249-63
references: 21431746
references: Environ Mol Mutagen. 2003;42(4):288-98
references: 14673874
references: J Cell Sci. 2007 Jul 15;120(Pt 14):2301-7
references: 17606985
references: Nat Cell Biol. 2003 Mar;5(3):255-60
references: 12598907
references: Mol Cell. 1998 Oct;2(4):437-45
references: 9809065
references: J Cell Biol. 2006 Apr 24;173(2):195-206
references: 16618811