title: Phage typing, PCR amplification for mecA gene, and antibiotic resistance patterns as epidemiologic markers in nosocomial outbreaks of methicillin resistant Staphylococcus aureus.
abstract: Staphylococcus aureus is one of the major causes of community and hospital-acquired infections. Bacteriophage considered as a major risk factor acquires S. aureus new virulence genetic elements. A total number of 119 S. aureus isolated from different specimens obtained from (RKH) were distinguished by susceptibility to 19 antimicrobial agents, phage typing, and PCR amplification for mecA gene. All of MRSA isolates harbored mecA gene, except three unique isolates. The predominant phage group is belonging to the (mixed group). Phage group (II) considered as an epidemiological marker correlated to β-lactamase hyper producer isolates. MRSA isolates indicated high prevalence of phage group (II) with highly increase for phage types (Ø3A), which were correlated to the skin. Phage types (Ø80/Ø81) played an important roll in Community Acquired Methicillin Resistant S. aureus (CAMRSA). Three outpatients MRSA isolates had low multiresistance against Bacitracin (Ba) and Fusidic acid (FD), considered as CAMRSA isolates. It was detected that group I typed all FD-resistant MSSA isolates. Phage groups (M) and (II) were found almost to be integrated for Gentamycin (GN) resistance especially phage type (Ø95) which relatively increased up to 20% in MRSA. Tetracycline (TE) resistant isolates typed by groups (II) and (III) in MSSA. Only one isolate resistant to Sulphamethoxazole/Trimethoprim (SXT) was typed by (III/V) alone in MSSA. MRSA isolates resistant to Chloramphenicol (C) and Ba were typed by all groups except (V). It could be concluded that (PERSA) S. aureus isolates from the wound that originated and colonized, and started to build up multi-resistance against the topical treatment antibiotics. In this study, some unique sporadic isolates for both MRSA and MSSA could be used as biological, molecular and epidemiological markers such as prospective tools.
authors: King Saud University, College of Science, Botany and Microbiology Department, P.O. Box 22452, Riyadh 11495, Saudi Arabia.
authors: M
authors: M
authors: Al-Khulaifi Manal
authors: M
authors: M
authors: Amin Aref Nagwa
authors: Ali A
authors: AA
authors: Al Salamah
keywords: Acquired infections; Community; Hospital; MRSA; MSSA; Multiresistance; Phage typing; Prospective tools; mecA
references: Eur J Epidemiol. 1997 Sep;13(6):699-708
references: 9324218
references: J Clin Microbiol. 1995 Jul;33(7):1712-5
references: 7665633
references: Proc Natl Acad Sci U S A. 2001 Aug 14;98(17):9865-70
references: 11481426
references: J Clin Microbiol. 1988 Sep;26(9):1907-9
references: 3183033
references: Eur J Clin Microbiol Infect Dis. 2001 Jun;20(6):425-7
references: 11476446
references: J Infect Dis. 1978 Mar;137(3):314-7
references: 147300
references: J Korean Med Sci. 2001 Aug;16(4):381-5
references: 11511780
references: J Bacteriol. 1965 Apr;89:988-92
references: 14276126
references: J Clin Microbiol. 1996 Sep;34(9):2267-9
references: 8862596
references: FEMS Microbiol Lett. 1998 May 15;162(2):265-74
references: 9627962
references: J Bacteriol. 1961 Dec;82:984-93
references: 13869737
references: J Hosp Infect. 1997 Aug;36(4):291-303
references: 9261759
references: Int J Antimicrob Agents. 2004 Mar;23(3):300-3
references: 15164973
references: Am J Ophthalmol. 2004 Mar;137(3):453-8
references: 15013867
references: Microbiol Rev. 1987 Mar;51(1):88-134
references: 3031442
references: Mol Microbiol. 1995 Jun;16(6):1231-41
references: 8577256
references: Int J Antimicrob Agents. 1999 Aug;12 Suppl 2:S35-44
references: 10528785
references: Int J Antimicrob Agents. 2002 Mar;19(3):241-3
references: 11932149
references: Neth J Med. 1998 Jun;52(6):219-27
references: 9718920
references: JAMA. 1998 Feb 25;279(8):593-8
references: 9486753
references: J Clin Microbiol. 1979 Oct;10(4):604-6
references: 160918
references: Scand J Infect Dis. 1992;24(4):401-9
references: 1411305
references: Methods Enzymol. 1987;152:145-70
references: 2958676
references: Zentralbl Bakteriol Mikrobiol Hyg A. 1981 Dec;251(2):171-6
references: 7200300
references: J Hosp Infect. 2003 Oct;55(2):116-8
references: 14529635
references: Int J Clin Pract. 2001 Jan-Feb;55(1):59-63
references: 11219321
references: J Bacteriol. 1984 May;158(2):513-6
references: 6563036
references: Clin Microbiol Rev. 1997 Oct;10(4):781-91
references: 9336672
references: Appl Microbiol. 1972 Jul;24(1):58-61
references: 4560474
references: Vet Microbiol. 2003 Aug 29;95(1-2):133-47
references: 12860083
references: Antimicrob Agents Chemother. 1991 Apr;35(4):632-9
references: 2069369
references: Antimicrob Agents Chemother. 1998 Dec;42(12):3163-8
references: 9835509
references: J Antimicrob Chemother. 1984 Dec;14 Suppl D:19-25
references: 6394576
references: Antimicrob Agents Chemother. 2003 Jan;47(1):196-203
references: 12499191
references: Vet Microbiol. 2003 Dec 2;97(1-2):63-72
references: 14637039
references: J Clin Microbiol. 1986 Sep;24(3):482-3
references: 3760143
references: Int J Antimicrob Agents. 2004 Mar;23(3):268-72
references: 15164968
references: J Postgrad Med. 1984 Jan;30(1):33-7
references: 6232376
references: Microbiol Rev. 1992 Sep;56(3):430-81
references: 1406491
references: Indian J Med Res. 2000 Mar;111:77-80
references: 10937382
references: Microb Drug Resist. 1996 Winter;2(4):435-41
references: 9158816
references: J Hosp Infect. 1995 Jun;30(2):111-24
references: 7673684