title: Detection of compatibility between baclofen and excipients with aid of infrared spectroscopy and chemometry.
abstract: In the paper infrared (IR) spectroscopy and multivariate exploration techniques: principal component analysis (PCA) and cluster analysis (CA) were applied as supportive methods for the detection of physicochemical incompatibilities between baclofen and excipients. In the course of research, the most useful rotational strategy in PCA proved to be varimax normalized, while in CA Ward's hierarchical agglomeration with Euclidean distance measure enabled to yield the most interpretable results. Chemometrical calculations confirmed the suitability of PCA and CA as the auxiliary methods for interpretation of infrared spectra in order to recognize whether compatibilities or incompatibilities between active substance and excipients occur. On the basis of IR spectra and the results of PCA and CA it was possible to demonstrate that the presence of lactose, β-cyclodextrin and meglumine in binary mixtures produce interactions with baclofen. The results were verified using differential scanning calorimetry, differential thermal analysis, thermogravimetry/differential thermogravimetry and X-ray powder diffraction analyses.
authors: Department of Analytical Chemistry, Medical University of Gdansk, Gen. J. Hallera 107, 80-416 Gdansk, Poland.
authors: Barbara
authors: B
authors: Rojek
authors: Marek
authors: M
authors: Wesolowski
authors: Bogdan
authors: B
authors: Suchacz
mesh_terms: D001418:Baclofen / Q000737:chemistry*; D016000:Cluster Analysis; D005079:Excipients / Q000737:chemistry*; D058786:GABA-B Receptor Agonists / Q000737:chemistry*; D015999:Multivariate Analysis; D025341:Principal Component Analysis; D013055:Spectrophotometry, Infrared / Q000379:methods*
chemical_list: D005079:Excipients; D058786:GABA-B Receptor Agonists; D001418:Baclofen
keywords: Baclofen; Compatibility/incompatibility; Excipients; Infrared spectroscopy; Multivariate analysis