title: Preventive and therapeutic effects of adenanthin on experimental autoimmune encephalomyelitis by inhibiting NF-κB signaling.
abstract: Adenanthin, a diterpenoid isolated from the leaves of Isodon adenanthus, has been reported to possess antileukemic activity through targeting peroxiredoxin I/II. However, its other potential activities remain to be explored. Using myelin oligodendrocyte glycoprotein (MOG)35-55-induced experimental autoimmune encephalomyelitis (EAE), an animal model for multiple sclerosis, we report in this study that adenanthin exerts efficaciously preventive and therapeutic effects on EAE accompanied by significant restriction of infiltration of inflammatory cells and demyelination in CNS. Adenanthin-presented immunomodulatory effects on EAE are correlated with suppressed proliferation of MOG35-55-reactive T cells, decreased Th1 and Th17 cells, increased regulatory T cell populations, decreased production of serum proinflammatory cytokines, and reduced stimulatory capacity of APCs, which might be mediated by its inhibitory action on NF-κB signaling pathway. Our results propose that, as a novel NF-κB inhibitor, adenanthin has potent immunomodulatory activity for the treatment of multiple sclerosis and possibly other autoimmune disorders.
authors: Institute of Health Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences and Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China.
authors: Qian-Qian
authors: QQ
authors: Yin
authors: Chuan-Xu
authors: CX
authors: Liu
authors: Ying-Li
authors: YL
authors: Wu
authors: Shao-Fang
authors: SF
authors: Wu
authors: Yan
authors: Y
authors: Wang
authors: Xia
authors: X
authors: Zhang
authors: Xiao-Juan
authors: XJ
authors: Hu
authors: Jian-Xin
authors: JX
authors: Pu
authors: Ying
authors: Y
authors: Lu
authors: Hu-Chen
authors: HC
authors: Zhou
authors: Hong-Lin
authors: HL
authors: Wang
authors: Hong
authors: H
authors: Nie
authors: Han-Dong
authors: HD
authors: Sun
authors: Guo-Qiang
authors: GQ
authors: Chen
mesh_terms: D000818:Animals; D015153:Blotting, Western; D004224:Diterpenes / Q000494:pharmacology*; D024202:Electrophoretic Mobility Shift Assay; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000276:immunology / Q000378:metabolism*; D005434:Flow Cytometry; D007150:Immunohistochemistry; D008213:Lymphocyte Activation / Q000187:drug effects / Q000276:immunology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D016328:NF-kappa B / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction / Q000187:drug effects*; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology / Q000378:metabolism
chemical_list: D004224:Diterpenes; D016328:NF-kappa B; C466222:adenanthin F