title: Sodium metavanadate exhibits carcinogenic tendencies in vitro in immortalized human bronchial epithelial cells.
abstract: Pentavalent vanadium compounds induce intracellular changes in vitro that are consistent with those of other carcinogenic substances. While there is no clear evidence that vanadium compounds cause cancer in humans, vanadium pentoxide causes lung cancer in rodents after long-term inhalation exposures and in turn IARC has categorized it as a group 2B possible human carcinogen. The goal of this study was to investigate the carcinogenicity of NaVO3 in the human immortalized bronchial epithelial cell line, Beas-2B. Cells were treated with 10 μM NaVO3 for 5 weeks, with or without recovery time, followed by gene expression microarray analysis. In a separate experiment, cells were exposed to 1-10 μM NaVO3 for 4 weeks and then grown in soft agar to test for anchorage-independent growth. A dose-dependent increase in the number of colonies was observed. In scratch tests, NaVO3-transformed clones could repair a wound faster than controls. In a gene expression microarray analysis of soft agar clones there were 2010 differentially expressed genes (DEG) (adjusted p-value ≤ 0.05) in NaVO3-transformed clones relative to control clones. DEG from this experiment were compared with the DEG of 5 week NaVO3 exposure with or without recovery, all with adjusted p-values < 0.05, and 469 genes were altered in the same direction for transformed clones, 5 week NaVO3-treated cells, and the recovered cells. The data from this study imply that chronic exposure to NaVO3 causes changes that are consistent with cellular transformation including anchorage-independent growth, enhanced migration ability, and gene expression changes that were likely epigenetically inherited.
authors: New York University, Department of Environmental Medicine, 57 Old Forge Road, Tuxedo, New York, USA. max.costa@nyumc.org.
authors: Lisa
authors: L
authors: Passantino
authors: Alexandra B
authors: AB
authors: Muñoz
authors: Max
authors: M
authors: Costa
mesh_terms: D019313:BRCA1 Protein / Q000235:genetics / Q000378:metabolism; D015153:Blotting, Western; D001980:Bronchi / Q000473:pathology*; D063646:Carcinogenesis / Q000187:drug effects / Q000473:pathology*; D002461:Cell Line, Transformed; D002465:Cell Movement / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D016000:Cluster Analysis; D003114:Colony-Forming Units Assay; D051187:Desmocollins / Q000235:genetics / Q000378:metabolism; D004847:Epithelial Cells / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology*; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014638:Vanadates / Q000633:toxicity*
chemical_list: D019313:BRCA1 Protein; C081561:DSC2 protein, human; C490356:DSC3 protein, human; D051187:Desmocollins; D012333:RNA, Messenger; D014638:Vanadates
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