title: A novel method for sample delivery and testing of whole blood: gel strip PCR for point of care (POC) molecular diagnostics.
abstract: Testing of whole blood in miniaturized PCR is compromised by the opaque nature of whole blood that leads to physical masking of a fluorescent signal. We demonstrate a method to perform real-time PCR with whole blood that avoids interference from the opacity of whole blood.
authors: Department of Oncology, University of Alberta and Cross Cancer Institute, 11560 University Avenue, Edmonton, AB T6G 1Z2, Canada. lpilarsk@ualberta.ca.
authors: Dammika P
authors: DP
authors: Manage
authors: Jana
authors: J
authors: Lauzon
authors: Alexey
authors: A
authors: Atrazhev
authors: Xiaoli
authors: X
authors: Pang
authors: Linda M
authors: LM
authors: Pilarski
mesh_terms: D016477:Artifacts; D001769:Blood / Q000378:metabolism*; D001774:Blood Chemical Analysis; D005782:Gels; D006801:Humans; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D019095:Point-of-Care Systems; D016133:Polymerase Chain Reaction / Q000379:methods*; D011934:Reagent Strips / Q000737:chemistry*; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D005782:Gels; D011934:Reagent Strips