title: Identification of novel α-n-methylation of CENP-B that regulates its binding to the centromeric DNA.
abstract: The eukaryotic centromere is an essential chromatin region required for accurate segregation of sister chromatids during cell division. Centromere protein B (CENP-B) is a highly conserved protein which can bind to the 17-bp CENP-B box on the centromeric DNA. In this study, we found that CENP-B could be α-N-methylated in human cells. We also showed that the level of the α-N-methylation was stimulated in cells in response to a variety of extracellular stimuli, including increased cell density, heat shock, and arsenite treatment, although the methylation level was not altered upon metaphase arrest. We identified N-terminal RCC1 methyltransferase (NRMT) as a major enzyme required for the CENP-B methylation. Additionally, we found that chromatin-bound CENP-B was primarily trimethylated and α-N-trimethylation could enhance CENP-B's binding to CENP-B box in cells. Our study also expands the function of protein α-N-methylation that has been known for decades and whose function remains largely unexplored.
authors: Department of Chemistry, University of California, Riverside, California 92521-0403, United States.
authors: Xiaoxia
authors: X
authors: Dai
authors: Koichiro
authors: K
authors: Otake
authors: Changjun
authors: C
authors: You
authors: Qian
authors: Q
authors: Cai
authors: Zi
authors: Z
authors: Wang
authors: Hiroshi
authors: H
authors: Masumoto
authors: Yinsheng
authors: Y
authors: Wang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002503:Centromere / Q000378:metabolism*; D051936:Centromere Protein B / Q000737:chemistry / Q000378:metabolism*; D004247:DNA / Q000378:metabolism*; D015870:Gene Expression; D057809:HEK293 Cells; D006801:Humans; D008745:Methylation; D008780:Methyltransferases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D051379:Mice; D011485:Protein Binding; D011499:Protein Processing, Post-Translational; D017434:Protein Structure, Tertiary
chemical_list: D051936:Centromere Protein B; D004247:DNA; D008780:Methyltransferases; C551913:NTMT1 protein, human
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