title: Histone acetyltransferase PCAF up-regulated cell apoptosis in hepatocellular carcinoma via acetylating histone H4 and inactivating AKT signaling.
abstract: BACKGROUND
  PCAF is an important intrinsic histone acetyltransferases. This study tried to establish the effect of PCAF on HCC cell apoptosis.
  
  
  METHODS
  Both in vitro and in vivo experiments including IHC, DAPI staining, caspase 3/7 activity assay, BrdU assay, MTT assay, western immunoblotting and co-immunoprecipitation were used here.
  
  
  RESULTS
  PCAF was found to be expressed at the low level in most of HCC cell lines. PCAF overexpression induced cell apoptosis and growth arrest with increased Histone H4 acetylation and inactivation of AKT signaling in Huh7 and HepG2 cells. The opposite results were obtained by silencing PCAF in Hep3B cells. The co-immunoprecipitation assay confirmed that PCAF protein was bound with histone H4 protein in the nucleus of Hep3B cells. Finally, the in vivo experiment confirmed the findings mentioned-above.
  
  
  CONCLUSIONS
  These data identified PCAF promotes cell apoptosis and functions as a HCC repressor through acetylating histone H4 and inactivating AKT signaling.
authors: Department of Hepatobiliary Surgery, the First Affiliated Hospital of Xi'an Jiaotong University, 277 Yanta West Road, Xi'an, Shaanxi 710061, China. raymondzhengxin@foxmail.com.
authors: Xin
authors: X
authors: Zheng
authors: Xiaohong
authors: X
authors: Gai
authors: Feihu
authors: F
authors: Ding
authors: Zhongtang
authors: Z
authors: Lu
authors: Kangsheng
authors: K
authors: Tu
authors: Yingmin
authors: Y
authors: Yao
authors: Qingguang
authors: Q
authors: Liu
mesh_terms: D000107:Acetylation; D000818:Animals; D017209:Apoptosis; D006528:Carcinoma, Hepatocellular / Q000201:enzymology* / Q000473:pathology; D049109:Cell Proliferation; D055785:Gene Knockdown Techniques; D056945:Hep G2 Cells; D006657:Histones / Q000378:metabolism*; D006801:Humans; D008114:Liver Neoplasms, Experimental / Q000378:metabolism* / Q000473:pathology; D008297:Male; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D010766:Phosphorylation; D011499:Protein Processing, Post-Translational; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism*; D015398:Signal Transduction; D047368:Tumor Burden; D015854:Up-Regulation; D050880:p300-CBP Transcription Factors / Q000502:physiology*; D000099224:p300-CBP-Associated Factor
chemical_list: D006657:Histones; D051057:Proto-Oncogene Proteins c-akt; D050880:p300-CBP Transcription Factors; D000099224:p300-CBP-Associated Factor
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