title: Analysis of in vivo occupancy of Aebp1, a transcription factor, using high resolution tiling array.
abstract: Aebp1 is a transcription factor that negatively regulates adipogenesis and also is upregulated in primary GBM as against secondary GBM. Here we describe a systematic approach to map the in vivo occupancy sites of Aebp1 by using a combination of chromatin immune-precipitation technique and hybridization to Agilent high resolution human promoter tiling array (2×224) containing 474393 probes. We also describe the use of various bioinformatics tools for analysis of the hybridization data and identification of Aebp1 occupancy sites in U87MG glioma cell line.
authors: Molecular Biology and Genetics Unit, Chromatin Biology Laboratory, Jawaharlal Nehru Centre for Advanced Scientific Research, Bangalore, India.
authors: Swati
authors: S
authors: Sinha
authors: Sainitin
authors: S
authors: Donakonda
authors: Satyanarayana Mr
authors: SM
authors: Rao
mesh_terms: D001665:Binding Sites; D002268:Carboxypeptidases / Q000378:metabolism*; D045744:Cell Line, Tumor; D047369:Chromatin Immunoprecipitation / Q000379:methods*; D019295:Computational Biology / Q000379:methods; D004247:DNA / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D005910:Glioma / Q000235:genetics; D006801:Humans; D009693:Nucleic Acid Hybridization / Q000379:methods*; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D012097:Repressor Proteins / Q000378:metabolism*
chemical_list: C484818:AEBP1 protein, human; D012097:Repressor Proteins; D004247:DNA; D002268:Carboxypeptidases