title: Lysobacter enzymogenes uses two distinct cell-cell signaling systems for differential regulation of secondary-metabolite biosynthesis and colony morphology.
abstract: Lysobacter enzymogenes is a ubiquitous environmental bacterium that is emerging as a potentially novel biological control agent and a new source of bioactive secondary metabolites, such as the heat-stable antifungal factor (HSAF) and photoprotective polyene pigments. Thus far, the regulatory mechanism(s) for biosynthesis of these bioactive secondary metabolites remains largely unknown in L. enzymogenes. In the present study, the diffusible signal factor (DSF) and diffusible factor (DF)-mediated cell-cell signaling systems were identified for the first time from L. enzymogenes. The results show that both Rpf/DSF and DF signaling systems played critical roles in modulating HSAF biosynthesis in L. enzymogenes. Rpf/DSF signaling and DF signaling played negative and positive effects in polyene pigment production, respectively, with DF playing a more important role in regulating this phenotype. Interestingly, only Rpf/DSF, but not the DF signaling system, regulated colony morphology of L. enzymgenes. Both Rpf/DSF and DF signaling systems were involved in the modulation of expression of genes with diverse functions in L. enzymogenes, and their own regulons exhibited only a few loci that were regulated by both systems. These findings unveil for the first time new roles of the Rpf/DSF and DF signaling systems in secondary metabolite biosynthesis of L. enzymogenes.
authors: College of Plant Protection and Key Laboratory of Integrated Management of Crop Diseases and Pests, Nanjing Agricultural University, Nanjing, China.
authors: Guoliang
authors: G
authors: Qian
authors: Yulan
authors: Y
authors: Wang
authors: Yiru
authors: Y
authors: Liu
authors: Feifei
authors: F
authors: Xu
authors: Ya-Wen
authors: YW
authors: He
authors: Liangcheng
authors: L
authors: Du
authors: Vittorio
authors: V
authors: Venturi
authors: Jiaqin
authors: J
authors: Fan
authors: Baishi
authors: B
authors: Hu
authors: Fengquan
authors: F
authors: Liu
mesh_terms: D001427:Bacterial Toxins / Q000096:biosynthesis*; D004926:Escherichia coli; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology*; D053528:Lysobacter / Q000235:genetics / Q000502:physiology*; D046228:Microarray Analysis; D056265:Microbial Interactions / Q000502:physiology*; D010860:Pigments, Biological / Q000096:biosynthesis*; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D064210:Secondary Metabolism / Q000235:genetics / Q000502:physiology*; D014974:Xanthomonas
chemical_list: D001427:Bacterial Toxins; D010860:Pigments, Biological
references: Mol Microbiol. 1997 May;24(3):555-66
references: 9179849
references: Mol Microbiol. 2000 Dec;38(5):986-1003
references: 11123673
references: Biostatistics. 2003 Apr;4(2):249-64
references: 12925520
references: Nucleic Acids Res. 2003 Feb 15;31(4):e15
references: 12582260
references: J Am Chem Soc. 2011 Feb 2;133(4):643-5
references: 21171605
references: Trends Microbiol. 2007 Aug;15(8):363-71
references: 17627825
references: Mol Microbiol. 2013 Jun;88(6):1058-69
references: 23617851
references: Curr Opin Plant Biol. 2002 Aug;5(4):285-90
references: 12179960
references: J Bacteriol. 1963 Nov;86:1070-4
references: 14080773
references: Mol Microbiol. 2006 Jan;59(2):610-22
references: 16390454
references: Mol Plant Microbe Interact. 2008 Oct;21(10):1309-15
references: 18785826
references: J Biol Chem. 2006 Nov 3;281(44):33414-21
references: 16940295
references: Microbiology (Reading). 2008 Jul;154(Pt 7):1845-1858
references: 18599814
references: Microbiol Res. 2003;158(2):107-15
references: 12906383
references: Mol Microbiol. 2007 Apr;64(2):281-92
references: 17378922
references: BMC Microbiol. 2012 Mar 09;12:30
references: 22404951
references: Mol Microbiol. 2004 Feb;51(3):903-12
references: 14731288
references: Curr Top Microbiol Immunol. 1994;192:29-41
references: 7859511
references: Annu Rev Genet. 2001;35:439-68
references: 11700290
references: J Mass Spectrom. 2007 Apr;42(4):490-6
references: 17295415
references: BMC Microbiol. 2010 Jul 09;10:187
references: 20615263
references: J Bacteriol. 1997 Jan;179(2):439-44
references: 8990296
references: J Bacteriol. 1994 Jan;176(2):269-75
references: 8288518
references: Annu Rev Microbiol. 2001;55:165-99
references: 11544353
references: Antimicrob Agents Chemother. 2011 Dec;55(12):5581-9
references: 21930890
references: J Virol. 2005 Nov;79(22):14392-403
references: 16254373
references: FEMS Microbiol Rev. 2009 Jul;33(4):739-56
references: 19222586
references: J Bacteriol. 2007 Jul;189(13):4964-8
references: 17468254
references: Nat Prod Rep. 2012 Nov;29(11):1277-87
references: 22898908
references: Mol Plant Microbe Interact. 2012 Feb;25(2):165-79
references: 21995764
references: Trends Microbiol. 2011 Mar;19(3):145-52
references: 21227698
references: Mol Biol Cell. 2006 Mar;17(3):1218-27
references: 16394102
references: World J Microbiol Biotechnol. 2012 Feb;28(2):549-57
references: 22806850
references: ISME J. 2008 Jan;2(1):27-36
references: 18049456
references: J Bacteriol. 2002 Jul;184(13):3539-48
references: 12057948
references: Mol Microbiol. 2013 Jan;87(1):80-93
references: 23113660
references: Proc Natl Acad Sci U S A. 2006 Apr 25;103(17):6712-7
references: 16611728
references: Bioinformatics. 2003 Jan 22;19(2):185-93
references: 12538238
references: Mol Plant Microbe Interact. 2011 Aug;24(8):948-57
references: 21539432
references: Mol Plant Microbe Interact. 2012 Jun;25(6):789-801
references: 22352717
references: Phytopathology. 2008 Jun;98(6):695-701
references: 18944294
references: Appl Environ Microbiol. 2010 Jul;76(14):4675-83
references: 20511428
references: Antimicrob Agents Chemother. 2007 Jan;51(1):64-72
references: 17074795