title: [Chromosome banding analysis of peripheral blood lymphocytes stimulated with IL2 and CpG oligonucleotide DSP30 in patients with chronic lymphocytic leukemia].
abstract: BACKGROUND
  Chromosomal aberrations play an important role as prognostic factors in chronic lymphocytic leukemia (CLL). These aberrations are mostly detected by fluorescent in situ hybridization (FISH), as chromosomal banding analysis has been scarce due to low proliferative activity of malignant B-lymphocytes in vitro. In 2006, a new method using stimulation with IL-2 and CpG oligonucleotide DSP30 for metaphase generation in CLL was published [1]. The objective of our study was to verify the efficacy of stimulation and to evaluate if the method is suitable for routine diagnostics.
  
  
  METHODS
  In total, peripheral blood samples of 369 CLL patients were analyzed in parallel by chromosomal banding analysis and by FISH probes for 13q14, 11q22-23, CEP12 and 17p13.
  
  
  RESULTS
  Out of 369 patients, 307 (83%) were successfully stimulated for metaphase generation. Chromosomal aberrations were detected in 243 (79%) out of 307 patients evaluated by chromosomal banding analysis. Other aberrations that are not included into standard FISH panel were detected in patients karyotypes, e.g. del(6q), del(14q), t(14;18)(q32;q21), t(11;14)(q13;q32) and t(18;22)(q21;q11). One hundred and three (42%) patients showed complex aberrant karyotype not detected by FISH analysis.
  
  
  CONCLUSIONS
  Stimulation with IL-2 and oligonucleotide DSP30 is an efficient method how to induce proliferation of malignant B-lymphocytes and allows detection of a substantial number of chromosomal aberrations in addition to those detected by standard FISH panel. Using this method in routine diagnostics is helpful particularly in identification of patients with complex aberrant karyotype.
authors: Interní hematologická a onkologická klinika, Centrum molekulární bio­logie a genové terapie, LF MU a FN Brno. kristina.stepanovska@fnbrno.cz
authors: K
authors: K
authors: Stěpanovská
authors: G
authors: G
authors: Vaňková
authors: V
authors: V
authors: Némethová
authors: L
authors: L
authors: Tomášiková
authors: P
authors: P
authors: Smuhařová
authors: E
authors: E
authors: Divíšková
authors: V
authors: V
authors: Vallová
authors: P
authors: P
authors: Kuglík
authors: K
authors: K
authors: Plevová
authors: A
authors: A
authors: Oltová
authors: M
authors: M
authors: Doubek
authors: S
authors: S
authors: Pospíšilová
authors: J
authors: J
authors: Mayer
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002869:Chromosome Aberrations; D002871:Chromosome Banding; D002880:Chromosomes, Human, Pair 11; D002882:Chromosomes, Human, Pair 13; D005260:Female; D020869:Gene Expression Profiling; D015973:Gene Expression Regulation, Leukemic; D015326:Gene Rearrangement, B-Lymphocyte, Heavy Chain; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007376:Interleukin-2 / Q000494:pharmacology; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000235:genetics* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009841:Oligonucleotides; D014407:Tumor Cells, Cultured
chemical_list: C539572:CpG-ODN DSP30; D007376:Interleukin-2; D009841:Oligonucleotides