title: Antioxidant Activities and Tyrosinase Inhibitory Effects of Different Extracts from Pleurotus ostreatus Fruiting Bodies.
abstract: We evaluated the antioxidant activity and tyrosinase inhibitory effects of Pleurotus ostreatus fruiting bodies extracted with acetone, methanol, and hot water. The antioxidant activities were tested against β-carotene-linoleic acid, reducing power, 1,1-diphenyl-2-picrylhydrazyl free radical scavenging activity, and ferrous chelating ability. Furthermore, phenolic acid and flavonoid contents were also analyzed. The methanol extract showed the strongest β-carotene-linoleic acid inhibition as compared to the other exracts. The acetone extract (8 mg/mL) showed a significantly high reducing power of 1.54 than the other extracts. The acetone extract was more effective than other extracts for scavenging on 1,1-diphenyl-2-picrylhydrazyl radicals. The strongest chelating effect (85.66%) was obtained from the acetone extract at 1.0 mg/mL. The antioxidant activities of the extracts from the P. ostreatus fruiting bodies increased with increasing concentration. A high performance liquid chromatography analysis detected seven phenolic compounds, including gallic acid, protocatechuic acid, chlorogenic acid, naringenin, hesperetin, formononetin, and biochanin-A in an acetonitrile and 0.1 N hydrochloric acid (5: 1) solvent extract. The total phenolic compound concentration was 188 µg/g. Tyrosinase inhibition of the acetone, methanol, and hot water P. ostreatus extracts increased with increasing concentration. The results revealed that the methanol extract had good tyrosinase inhibitory ability, whereas the acetone and hot water extracts showed moderate activity at the concentrations tested. The results suggested that P. ostreatus may have potential as a natural antioxidant.
authors: Division of Life Sciences, University of Incheon, Incheon 406-840, Korea.
authors: Nuhu
authors: N
authors: Alam
authors: Ki Nam
authors: KN
authors: Yoon
authors: Kyung Rim
authors: KR
authors: Lee
authors: Pyung Gyun
authors: PG
authors: Shin
authors: Jong Chun
authors: JC
authors: Cheong
authors: Young Bok
authors: YB
authors: Yoo
authors: Ja Mi
authors: JM
authors: Shim
authors: Min Woong
authors: MW
authors: Lee
authors: U Youn
authors: UY
authors: Lee
authors: Tae Soo
authors: TS
authors: Lee
keywords: Antioxidant; Phenolic compounds; Pleurotus ostreatus; Tyrosinase inhibition
references: J Ethnopharmacol. 2003 May;86(1):51-8
references: 12686441
references: Crit Rev Food Sci Nutr. 1992;32(1):67-103
references: 1290586
references: Molecules. 2007 Apr 11;12(4):759-71
references: 17851428
references: Mycobiology. 2008 Dec;36(4):228-32
references: 23997631
references: Arch Biochem Biophys. 1994 Nov 15;315(1):161-9
references: 7979394
references: Food Chem Toxicol. 2008 Jul;46(7):2466-71
references: 18482785
references: J Ethnopharmacol. 2008 Oct 28;119(3):507-12
references: 18573327
references: Mutat Res. 1988 Dec;202(2):377-86
references: 3057368
references: Biosci Biotechnol Biochem. 2005 Jan;69(1):197-201
references: 15665485
references: Mycobiology. 2009 Mar;37(1):37-42
references: 23983505
references: Biochem J. 1984 Apr 1;219(1):1-14
references: 6326753
references: Food Chem Toxicol. 2007 Feb;45(2):328-36
references: 17049706