title: Characterization of interactions between heparin/glycosaminoglycan and adeno-associated virus.
abstract: Adeno-associated virus (AAV) is a key candidate in the development of gene therapy. In this work, we used surface plasmon resonance spectroscopy to study the interaction between AAV and heparin and other glycosaminoglycans (GAGs). Surface plasmon resonance results revealed that heparin binds to AAV with an extremely high affinity. Solution competition studies showed that binding of AAV to heparin is chain length-dependent. AAV prefers to bind full chain heparin. All sulfo groups (especially N-sulfo and 6-O-sulfo groups) on heparin are important for the AAV-heparin interaction. Higher levels of sulfo group substitution in GAGs enhance their binding affinities. Atomic force microscopy was also performed to image AAV-2 in a complex with heparin.
authors: Department of Chemical and Biological Engineering and ‡Department of Chemistry and Chemical Biology, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute , Troy, New York 12180, United States.
authors: Fuming
authors: F
authors: Zhang
authors: Javier
authors: J
authors: Aguilera
authors: Julie M
authors: JM
authors: Beaudet
authors: Qing
authors: Q
authors: Xie
authors: Thomas F
authors: TF
authors: Lerch
authors: Omar
authors: O
authors: Davulcu
authors: Wilfredo
authors: W
authors: Colón
authors: Michael S
authors: MS
authors: Chapman
authors: Robert J
authors: RJ
authors: Linhardt
mesh_terms: D000818:Animals; D000229:Dependovirus / Q000378:metabolism*; D006025:Glycosaminoglycans / Q000378:metabolism*; D006493:Heparin / Q000031:analogs & derivatives / Q000378:metabolism*; D018625:Microscopy, Atomic Force; D020349:Surface Plasmon Resonance; D013552:Swine
chemical_list: D006025:Glycosaminoglycans; D006493:Heparin
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