title: Possible involvement of oxidative stress as a causative factor in blood-brain barrier dysfunction in stroke-prone spontaneously hypertensive rats.
abstract: To elucidate the pathogenic roles of oxidative stress on blood-brain-barrier (BBB) dysfunction, we compared the chronological changes of oxidative stress in blood and cerebral tissue between stroke-prone spontaneously hypertensive rats (SHRSP) and normotensive Wistar-Kyoto rats (WKY). Plasma and tissue oxidative stress was assayed by the diacron-reactive oxygen metabolite (d-ROM) test using 8-hydroxy-2'-deoxyguanosine (8-OHdG) as a reference oxidative stress marker. The plasma and cerebral cortex d-ROM levels increased in SHRSP after 16weeks of age, but not in WKY. There were no significant differences in 8-OHdG or lipid peroxidation markers between SHRSP and WKY. Antioxidant capacity, as estimated by the biological antioxidant potential test, was similar between SHRSP and WKY at all ages examined. The changes in plasma and tissue d-ROM levels coincided with changes in glucose transporter-1 and aquaporin-4 expression, as functional constituents of the BBB. These results indicate that plasma oxidative stress increases before the onset of tissue damage, and plays an important role in BBB dysfunction rather than decreases in antioxidant capacity. The plasma d-ROM test appears to be useful for predicting vasogenic cerebral edema in severe hypertension.
authors: Department of Food Science and Nutrition, Faculty of Agriculture, Kinki University, Nara, Japan. Electronic address: kuriman@nara.kindai.ac.jp.
authors: Kumiko
authors: K
authors: Takemori
authors: Tetsuo
authors: T
authors: Murakami
authors: Takashi
authors: T
authors: Kometani
authors: Hiroyuki
authors: H
authors: Ito
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000818:Animals; D051401:Aquaporin 4 / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism; D001794:Blood Pressure; D001812:Blood-Brain Barrier / Q000378:metabolism* / Q000503:physiopathology; D001835:Body Weight; D001929:Brain Edema / Q000209:etiology* / Q000378:metabolism / Q000503:physiopathology; D002199:Capillary Permeability; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000378:metabolism; D004195:Disease Models, Animal; D051272:Glucose Transporter Type 1 / Q000378:metabolism; D006973:Hypertension / Q000150:complications* / Q000378:metabolism / Q000503:physiopathology; D015227:Lipid Peroxidation; D008297:Male; D018384:Oxidative Stress; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY; D020521:Stroke / Q000209:etiology* / Q000378:metabolism / Q000503:physiopathology; D013997:Time Factors
chemical_list: C496032:Aqp4 protein, rat; D051401:Aquaporin 4; D015415:Biomarkers; D051272:Glucose Transporter Type 1; C495679:Slc2a1 protein, rat; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine