title: Novel methods for studying multiprotein complexes in vivo.
abstract: The current consensus is that the majority of proteins act in concert in the cell, as homo- and heteromeric complexes of two or more proteins that carry out discrete biological functions. A wide range of genomic, proteomic, biochemical, structural and biophotonic techniques have been employed over the years to study the protein-protein interactions that define complexes, with the end goal of producing a spatiotemporal map of these modular functional units throughout the cell. Recent advances in the analysis of in vivo complexes have greatly improved structural, functional and temporal resolution, and this review highlights novel approaches ranging from proximity-dependent labeling and cross-linking/mass spectrometry through pulse-chase epitope labeling and targeted protein degradation.
authors: Department of Cellular and Molecular Medicine, Ottawa Institute of Systems Biology, University of Ottawa 451 Smyth Road, Ottawa, ON K1H 8M5 Canada.
authors: Virja
authors: V
authors: Mehta
authors: Laura
authors: L
authors: Trinkle-Mulcahy
references: Mol Cell Proteomics. 2012 Feb;11(2):M111.008318
references: 22067100
references: Nucleic Acids Res. 2013 Jan 7;41(1):e28
references: 23066101
references: J Cell Biol. 2008 Oct 20;183(2):223-39
references: 18936248
references: Nat Struct Mol Biol. 2011 Dec 11;19(1):117-21
references: 22157958
references: Anal Chem. 2010 Apr 1;82(7):2784-96
references: 20225856
references: Mass Spectrom Rev. 2010 Nov-Dec;29(6):862-76
references: 20730915
references: Proc Natl Acad Sci U S A. 2010 Dec 7;107(49):20992-7
references: 21098295
references: Mol Cell Proteomics. 2010 Aug;9(8):1650-65
references: 20445003
references: Science. 2012 Sep 14;337(6100):1348-52
references: 22984071
references: Nat Methods. 2009 Dec;6(12):917-22
references: 19915560
references: Mol Cell Proteomics. 2012 Mar;11(3):R111.014027
references: 22180098
references: Nat Rev Genet. 2001 Sep;2(9):659-68
references: 11533715
references: EMBO J. 2010 Feb 17;29(4):717-26
references: 20094031
references: Genetics. 2011 Aug;188(4):773-82
references: 21828278
references: Mol Cell. 2012 Sep 14;47(5):788-96
references: 22819325
references: Nat Rev Genet. 2013 Feb;14(2):100-12
references: 23329111
references: J Biol Chem. 2010 Apr 9;285(15):11039-44
references: 20147747
references: Front Biosci. 2006 Jan 01;11:313-27
references: 16146733
references: Cell. 2013 Apr 11;153(2):461-70
references: 23582331
references: Mol Cell Proteomics. 2010 Aug;9(8):1634-49
references: 20360032
references: Trends Cell Biol. 2008 Sep;18(9):443-50
references: 18706812
references: J Proteome Res. 2011 Aug 5;10(8):3604-16
references: 21618984
references: J Proteome Res. 2013 Apr 5;12(4):1569-79
references: 23413883
references: Proteomics. 2012 May;12(10):1623-38
references: 22610586
references: J Proteomics. 2013 Aug 2;88:120-8
references: 23541715
references: Nat Biotechnol. 2000 Apr;18(4):424-8
references: 10748524
references: Nat Rev Mol Cell Biol. 2007 Aug;8(8):645-54
references: 17593931
references: Adv Drug Deliv Rev. 2009 Jul 25;61(9):746-59
references: 19389436
references: Curr Opin Biotechnol. 2008 Aug;19(4):331-7
references: 18590817
references: Mol Cell. 2000 Sep;6(3):751-6
references: 11030355
references: J Cell Biol. 2012 Mar 19;196(6):801-10
references: 22412018
references: Proc Natl Acad Sci U S A. 2012 Dec 4;109(49):E3350-7
references: 23150568
references: Nat Chem Biol. 2012 Sep;8(9):748-50
references: 22864544
references: Nat Neurosci. 2007 Aug;10(8):1063-72
references: 17603477