title: Plasmacytoid dendritic cells are crucial in Bifidobacterium adolescentis-mediated inhibition of Yersinia enterocolitica infection.
abstract: In industrialized countries bacterial intestinal infections are commonly caused by enteropathogenic Enterobacteriaceae. The interaction of the microbiota with the host immune system determines the adequacy of an appropriate response against pathogens. In this study we addressed whether the probiotic Bifidobacterium adolescentis is protective during intestinal Yersinia enterocolitica infection. Female C57BL/6 mice were fed with B. adolescentis, infected with Yersinia enterocolitica, or B. adolescentis fed and subsequently infected with Yersinia enterocolitica. B. adolescentis fed and Yersinia infected mice were protected from Yersinia infection as indicated by a significantly reduced weight loss and splenic Yersinia load when compared to Yersinia infected mice. Moreover, protection from infection was associated with increased intestinal plasmacytoid dendritic cell and regulatory T-cell frequencies. Plasmacytoid dendritic cell function was investigated using depletion experiments by injecting B. adolescentis fed, Yersinia infected C57BL/6 mice with anti-mouse PDCA-1 antibody, to deplete plasmacytoid dendritic cells, or respective isotype control. The B. adolescentis-mediated protection from Yersinia dissemination to the spleen was abrogated after plasmacytoid dendritic cell depletion indicating a crucial function for pDC in control of intestinal Yersinia infection. We suggest that feeding of B. adolescentis modulates the intestinal immune system in terms of increased plasmacytoid dendritic cell and regulatory T-cell frequencies, which might account for the B. adolescentis-mediated protection from Yersinia enterocolitica infection.
authors: Institute for Medical Microbiology and Hygiene, University Hospital Tübingen, Tübingen, Germany ; German Centre for Infection Research, University of Tübingen, Tübingen, Germany.
authors: Alexandra
authors: A
authors: Wittmann
authors: Ingo B
authors: IB
authors: Autenrieth
authors: Julia-Stefanie
authors: JS
authors: Frick
mesh_terms: D000818:Animals; D000898:Antibiosis / Q000276:immunology*; D000906:Antibodies / Q000009:adverse effects; D001644:Bifidobacterium / Q000276:immunology*; D001835:Body Weight; D003713:Dendritic Cells / Q000166:cytology / Q000187:drug effects / Q000276:immunology*; D005260:Female; D007113:Immunity, Innate; D056747:Immunomodulation; D051379:Mice; D008810:Mice, Inbred C57BL; D019936:Probiotics / Q000008:administration & dosage*; D013154:Spleen / Q000166:cytology / Q000276:immunology; D050378:T-Lymphocytes, Regulatory / Q000166:cytology / Q000276:immunology; D015009:Yersinia Infections / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control*; D015008:Yersinia enterocolitica / Q000254:growth & development* / Q000472:pathogenicity
chemical_list: D000906:Antibodies
references: Immunity. 2009 Feb 20;30(2):289-99
references: 19217323
references: PLoS Negl Trop Dis. 2011 Aug;5(8):e1269
references: 21858239
references: J Immunol. 2012 Aug 1;189(3):1243-52
references: 22753934
references: Infect Immun. 2009 Jun;77(6):2367-75
references: 19307217
references: N Engl J Med. 1989 Jul 6;321(1):16-24
references: 2659991
references: J Immunol. 2010 Apr 1;184(7):3478-86
references: 20190134
references: Infect Immun. 2003 May;71(5):2839-58
references: 12704158
references: Annu Rev Cell Dev Biol. 2000;16:301-32
references: 11031239
references: ISME J. 2007 May;1(1):56-66
references: 18043614
references: Curr Top Microbiol Immunol. 1994;192:243-63
references: 7859508
references: Nature. 2008 Oct 9;455(7214):804-7
references: 18724361
references: Immunol Rev. 2010 Mar;234(1):268-81
references: 20193025
references: Eur J Immunol. 2010 Oct;40(10):2667-76
references: 20821731
references: J Exp Med. 2006 Jun 12;203(6):1591-601
references: 16754724
references: Am J Physiol Gastrointest Liver Physiol. 2004 Apr;286(4):G613-26
references: 15010363
references: Gastroenterology. 2004 Jul;127(1):224-38
references: 15236188
references: Infect Immun. 2004 Oct;72(10):5750-8
references: 15385474
references: Pediatr Infect Dis J. 2012 Aug;31(8):859-62
references: 22801095
references: Curr Top Microbiol Immunol. 2013;358:273-89
references: 22476557
references: J Antimicrob Chemother. 2006 Dec;58(6):1160-7
references: 17046967
references: J Microbiol Biotechnol. 2008 Jul;18(7):1278-85
references: 18667857
references: J Immunol. 2008 May 1;180(9):5843-52
references: 18424703
references: PLoS Biol. 2008 Nov 18;6(11):e280
references: 19018661
references: Immunity. 2009 Sep 18;31(3):513-25
references: 19733489
references: J Immunol. 2004 Oct 1;173(7):4433-42
references: 15383574
references: Microbes Infect. 1999 Apr;1(4):323-33
references: 10602666
references: Science. 2005 Jan 14;307(5707):254-8
references: 15653504
references: J Gastroenterol Hepatol. 2000 May;15(5):489-93
references: 10847433
references: Gastroenterology. 2007 Feb;132(2):562-75
references: 17258729
references: Annu Rev Immunol. 2007;25:381-418
references: 17378762
references: PLoS Biol. 2007 Oct;5(10):2177-89
references: 17760501
references: Annu Rev Immunol. 2009;27:551-89
references: 19302048
references: Immunity. 2008 Sep 19;29(3):352-61
references: 18799143
references: Cell Host Microbe. 2007 Sep 13;2(3):204
references: 18030708
references: Proc Natl Acad Sci U S A. 2010 Jan 5;107(1):228-33
references: 19966295
references: Annu Rev Immunol. 2009;27:313-38
references: 19302043
references: J Immunol. 2007 Sep 1;179(5):2690-4
references: 17709480
references: Clin Exp Immunol. 2011 Oct;166(1):46-54
references: 21762123
references: J Biosci Bioeng. 2005 Dec;100(6):583-92
references: 16473765
references: Microb Ecol. 2008 Oct;56(3):395-402
references: 18209965
references: Immunity. 2012 Sep 21;37(3):511-23
references: 22981537
references: Immunity. 2013 Mar 21;38(3):581-95
references: 23395676
references: J Proteome Res. 2008 Sep;7(9):3718-28
references: 18698804
references: Nature. 2009 Oct 29;461(7268):1282-6
references: 19865172
references: Cell Commun Signal. 2011 Nov 01;9(1):25
references: 22044597
references: Gut. 1987 Oct;28(10):1221-7
references: 3678950
references: Nat Immunol. 2008 Nov;9(11):1253-60
references: 18836452
references: Nature. 2011 Jan 27;469(7331):543-7
references: 21270894
references: Blood. 2012 Jun 21;119(25):6063-71
references: 22547585
references: Clin Microbiol Infect. 2005 Dec;11(12):958-66
references: 16307549
references: Infect Immun. 1994 Jun;62(6):2590-9
references: 8188382
references: J Exp Med. 2007 Jan 22;204(1):105-15
references: 17200410
references: Immunity. 2012 Feb 24;36(2):276-87
references: 22306017
references: Infect Immun. 2007 Jul;75(7):3490-7
references: 17485456
references: Gut. 2013 Feb;62(2):220-6
references: 22345653
references: J Clin Invest. 2005 Mar;115(3):695-702
references: 15765149
references: PLoS One. 2011 Feb 18;6(2):e16967
references: 21365015
references: J Immunol. 2012 Nov 15;189(10):4900-8
references: 23071281
references: Physiol Rev. 2010 Jul;90(3):859-904
references: 20664075
references: PLoS Pathog. 2012;8(10):e1002995
references: 23133377
references: BMJ. 2007 Aug 18;335(7615):340
references: 17690340
references: Proc Natl Acad Sci U S A. 2007 Apr 10;104(15):6347-52
references: 17404233
references: Infect Immun. 2008 Oct;76(10):4726-36
references: 18678663
references: Cell. 2005 Jul 15;122(1):107-18
references: 16009137
references: Foodborne Pathog Dis. 2009 Mar;6(2):251-5
references: 19099355