title: Urine kidney injury molecule-1: a potential non-invasive biomarker for patients with renal cell carcinoma.
abstract: BACKGROUND
  KIM-1 staining is upregulated in proximal tubule-derived renal cell carcinoma (RCC) including clear renal cell carcinoma and papillary renal cell carcinoma, but not in chromophobe RCC (distal tubular tumor). This study was designed to prospectively examine urine KIM-1 level before and 1 month after removal of renal tumors.
  
  
  METHODS
  A total of 19 patients were eventually enrolled in the study based on pre-operative imaging studies. Pre-operative and follow-up (1 month) urine KIM-1 levels were measured. The urine KIM-1 levels (uKIM-1) were then normalized to urine creatinine levels (uCr). Renal tumors were also stained for KIM-1 using immunohistochemical techniques.
  
  
  RESULTS
  The KIM-1-negative staining group included 7 cases, and the KIM-1-positive group consisted of 12 cases. The percentage of KIM-1-positive staining RCC cells ranged from 10 to 100 %, and the staining intensity ranged from 1+ to 3+. In both groups, serum creatinine levels were both significantly elevated after nephrectomy. In the KIM-1-negative group, uKIM-1/uCr remained at a similar level before (0.37 ± 0.1 ng/mg Cr) and after nephrectomy (0.32 ± 0.01 ng/mg Cr). However, in the KIM-1-positive group, elevated uKIM-1/uCr at 1.20 ± 0.31 ng/mg Cr was significantly reduced to 0.36 ± 0.1 ng/mg Cr, which was similar to the pre-operative uKIM-1/uCr (0.37 ± 0.1 ng/mg Cr) in the KIM-1-negative group.
  
  
  CONCLUSIONS
  Our small but prospective study showed significant reduction in uKIM-1/uCr after nephrectomy in the KIM-1 positive group, suggesting that urine KIM-1 may serve as a surrogate biomarker for kidney cancer and a non-invasive pre-operative measure to evaluate the malignant potential of renal masses.
authors: Department of Anatomic Pathology, William Beaumont Hospital, 3601 W. 13 Mile Rd, Royal Oak, MI, USA.
authors: Ping L
authors: PL
authors: Zhang
authors: Joseph W
authors: JW
authors: Mashni
authors: Venkata S
authors: VS
authors: Sabbisetti
authors: Charles M
authors: CM
authors: Schworer
authors: George D
authors: GD
authors: Wilson
authors: Stacy C
authors: SC
authors: Wolforth
authors: Kenneth M
authors: KM
authors: Kernen
authors: Brian D
authors: BD
authors: Seifman
authors: Mitual B
authors: MB
authors: Amin
authors: Timothy J
authors: TJ
authors: Geddes
authors: Fan
authors: F
authors: Lin
authors: Joseph V
authors: JV
authors: Bonventre
authors: Jason M
authors: JM
authors: Hafron
mesh_terms: D000368:Aged; D015703:Antigens, CD / Q000032:analysis; D015214:Antigens, Differentiation, Myelomonocytic / Q000032:analysis; D015415:Biomarkers / Q000032:analysis / Q000652:urine; D002292:Carcinoma, Renal Cell / Q000737:chemistry / Q000235:genetics / Q000473:pathology / Q000652:urine*; D003404:Creatinine / Q000652:urine; D005260:Female; D000072596:Hepatitis A Virus Cellular Receptor 1; D006801:Humans; D007680:Kidney Neoplasms / Q000737:chemistry / Q000235:genetics / Q000473:pathology / Q000652:urine*; D007687:Kidney Tubules, Proximal; D008297:Male; D008562:Membrane Glycoproteins / Q000032:analysis / Q000235:genetics / Q000652:urine*; D008875:Middle Aged; D009392:Nephrectomy; D011446:Prospective Studies; D011991:Receptors, Virus / Q000032:analysis / Q000235:genetics; D000097382:CD68 Molecule
chemical_list: D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D015415:Biomarkers; D003404:Creatinine; D000072596:Hepatitis A Virus Cellular Receptor 1; D008562:Membrane Glycoproteins; D011991:Receptors, Virus; C067980:CD68 antigen, human; D000097382:CD68 Molecule; C491143:HAVCR1 protein, human
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