title: TLR4 gene expression in pig populations and its association with resistance to Escherichia coli F18.
abstract: TLR4 is the main recognition receptor of bacterial lipopolysaccharides, which play an important role in innate and adaptive immunity. We used real-time PCR to analyze the tissue expression profile and differential expression of TLR4 in 4 pig populations (Escherichia coli F18-resistant Sutai, E. coli F18-sensitive Sutai, Large White, Meishan), in order to determine the role that the TLR4 gene plays in resistance to E. coli F18. We found that TLR4 expressed consistently in the 4 populations, with relatively high levels in immune tissues and the highest level in the lung. Generally, the expression of TLR4 in E. coli F18-sensitive individuals was the highest, followed by that in E. coli F18-resistant, Large White and Meishan. In the spleen, lung, kidney, lymph nodes, and thymus gland, TLR4 expression is significantly higher in the E. coli F18-sensitive than in the other 3 populations; there were no significant differences among E. coli F18-resistant Sutai, Large White, and Meishan. In addition, Gene Ontology and pathway analysis showed that TLR4 takes part in the inflammatory response. We found that porcine TLR4 has consistent tissue specificity in each breed, and downregulation of expression of the TLR4 gene is related to resistance to E. coli F18 in weaning piglets.
authors: College of Animal Science and Technology, Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design of Jiangsu Province, Yangzhou University, Yangzhou, China.
authors: J
authors: J
authors: Wang
authors: Z Y
authors: ZY
authors: Pan
authors: X R
authors: XR
authors: Zheng
authors: Z C
authors: ZC
authors: Wu
authors: X M
authors: XM
authors: Su
authors: G Q
authors: GQ
authors: Zhu
authors: X G
authors: XG
authors: Huang
authors: S L
authors: SL
authors: Wu
authors: W B
authors: WB
authors: Bao
mesh_terms: D000818:Animals; D017849:Animals, Inbred Strains; D060467:Disease Resistance / Q000235:genetics*; D015536:Down-Regulation; D004927:Escherichia coli Infections / Q000235:genetics* / Q000276:immunology; D056726:Genetic Association Studies; D007113:Immunity, Innate / Q000235:genetics; D009928:Organ Specificity; D011153:Population / Q000235:genetics; D013552:Swine / Q000235:genetics*; D051197:Toll-Like Receptor 4 / Q000235:genetics* / Q000378:metabolism; D014158:Transcription, Genetic
chemical_list: D051197:Toll-Like Receptor 4