title: Adaptive response in human blood lymphocytes exposed to non-ionizing radiofrequency fields: resistance to ionizing radiation-induced damage.
abstract: The aim of this preliminary investigation was to assess whether human peripheral blood lymphocytes which have been pre-exposed to non-ionizing radiofrequency fields exhibit an adaptive response (AR) by resisting the induction of genetic damage from subsequent exposure to ionizing radiation. Peripheral blood lymphocytes from four healthy donors were stimulated with phytohemagglutinin for 24 h and then exposed for 20 h to 1950 MHz radiofrequency fields (RF, adaptive dose, AD) at an average specific absorption rate of 0.3 W/kg. At 48 h, the cells were subjected to a challenge dose (CD) of 1.0 or 1.5 Gy X-irradiation (XR, challenge dose, CD). After a 72 h total culture period, cells were collected to examine the incidence of micronuclei (MN). There was a significant decrease in the number of MN in lymphocytes exposed to RF + XR (AD + CD) as compared with those subjected to XR alone (CD). These observations thus suggested a RF-induced AR and induction of resistance to subsequent damage from XR. There was variability between the donors in RF-induced AR. The data reported in our earlier investigations also indicated a similar induction of AR in human blood lymphocytes that had been pre-exposed to RF (AD) and subsequently treated with a chemical mutagen, mitomycin C (CD). Since XR and mitomycin-C induce different kinds of lesions in cellular DNA, further studies are required to understand the mechanism(s) involved in the RF-induced adaptive response.
authors: CNR - Institute for Electromagnetic Sensing of the Environment, via Diocleziano 328, 80124, Napoli, Italy.
authors: Anna
authors: A
authors: Sannino
authors: Olga
authors: O
authors: Zeni
authors: Stefania
authors: S
authors: Romeo
authors: Rita
authors: R
authors: Massa
authors: Giancarlo
authors: G
authors: Gialanella
authors: Gianfranco
authors: G
authors: Grossi
authors: Lorenzo
authors: L
authors: Manti
authors: Vijayalaxmi
authors: Maria Rosaria
authors: MR
authors: Scarfì
mesh_terms: D000222:Adaptation, Physiological / Q000235:genetics* / Q000528:radiation effects*; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D006801:Humans; D008214:Lymphocytes / Q000166:cytology / Q000502:physiology* / Q000528:radiation effects*; D048629:Micronuclei, Chromosome-Defective / Q000528:radiation effects*; D011836:Radiation Tolerance / Q000235:genetics* / Q000528:radiation effects*; D011846:Radio Waves
keywords: X-rays; adaptive response; human lymphocytes; micronucleus; radiofrequency
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