title: Human and animal isolates of Yersinia enterocolitica show significant serotype-specific colonization and host-specific immune defense properties.
abstract: Yersinia enterocolitica is a human pathogen that is ubiquitous in livestock, especially pigs. The bacteria are able to colonize the intestinal tract of a variety of mammalian hosts, but the severity of induced gut-associated diseases (yersiniosis) differs significantly between hosts. To gain more information about the individual virulence determinants that contribute to colonization and induction of immune responses in different hosts, we analyzed and compared the interactions of different human- and animal-derived isolates of serotypes O:3, O:5,27, O:8, and O:9 with murine, porcine, and human intestinal cells and macrophages. The examined strains exhibited significant serotype-specific cell binding and entry characteristics, but adhesion and uptake into different host cells were not host specific and were independent of the source of the isolate. In contrast, survival and replication within macrophages and the induced proinflammatory response differed between murine, porcine, and human macrophages, suggesting a host-specific immune response. In fact, similar levels of the proinflammatory cytokine macrophage inflammatory protein 2 (MIP-2) were secreted by murine bone marrow-derived macrophages with all tested isolates, but the equivalent interleukin-8 (IL-8) response of porcine bone marrow-derived macrophages was strongly serotype specific and considerably lower in O:3 than in O:8 strains. In addition, all tested Y. enterocolitica strains caused a considerably higher level of secretion of the anti-inflammatory cytokine IL-10 by porcine than by murine macrophages. This could contribute to limiting the severity of the infection (in particular of serotype O:3 strains) in pigs, which are the primary reservoir of Y. enterocolitica strains pathogenic to humans.
authors: Department of Molecular Infection Biology, Helmholtz Centre for Infection Research, Braunschweig, Germany.
authors: Julia
authors: J
authors: Schaake
authors: Malte
authors: M
authors: Kronshage
authors: Frank
authors: F
authors: Uliczka
authors: Manfred
authors: M
authors: Rohde
authors: Tobias
authors: T
authors: Knuuti
authors: Eckhard
authors: E
authors: Strauch
authors: Angelika
authors: A
authors: Fruth
authors: Melissa
authors: M
authors: Wos-Oxley
authors: Petra
authors: P
authors: Dersch
mesh_terms: D000818:Animals; D001422:Bacterial Adhesion; D002478:Cells, Cultured; D016207:Cytokines / Q000378:metabolism; D004705:Endocytosis; D004847:Epithelial Cells / Q000276:immunology / Q000382:microbiology; D005260:Female; D054884:Host-Pathogen Interactions; D006801:Humans; D008264:Macrophages / Q000276:immunology / Q000382:microbiology; D051379:Mice; D008810:Mice, Inbred C57BL; D050296:Microbial Viability; D012703:Serotyping; D013552:Swine; D015008:Yersinia enterocolitica / Q000145:classification* / Q000276:immunology* / Q000302:isolation & purification
chemical_list: D016207:Cytokines
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