title: Effect of a novel nuclear factor-κB activation inhibitor on renal ischemia-reperfusion injury.
abstract: BACKGROUND
  In kidney transplantation, the relationship between prolonged warm or cold ischemic storage of kidneys and a higher incidence of delayed graft function is previously known, and delayed graft function has been known to aggravate poor long-term graft survival. We investigated the effect of a novel nuclear factor-κB activation inhibitor, dehydroxymethylepoxyquinomicin (DHMEQ), on renal ischemia-reperfusion (I/R) injury.
  
  
  METHODS
  DHMEQ was administered to Lewis rats once just before renal artery clamping (DHMEQ pretreatment group), and the effect on I/R injury was investigated.
  
  
  RESULTS
  In the DHMEQ pretreatment group, the 24-hr urine volume on days 1 to 3 after I/R was significantly larger, and the protein concentration of the urine on days 2 to 7 was significantly smaller than in the untreated group. The serum creatinine level was significantly improved, and significantly lower levels of the inflammatory cells and inflammatory cytokines were present in the kidneys on day 1. The relative ratio of nuclear to cytoplasmic nuclear factor-κB and oxidative stress of kidney tissue on day 1 were significantly decreased.
  
  
  CONCLUSIONS
  Treatment with DHMEQ before renal artery clamping may therefore be useful for renal I/R injury and application to renal transplantation is expected.
authors: 1 Department of Urology, Keio University School of Medicine, Tokyo, Japan. 2 Department of Molecular Target Medicine Screening, Aichi Medical University, Nagakute, Japan. 3 Address correspondence to: Ken Nakagawa, M.D., Department of Urology, Keio University School of Medicine, Shinanomachi 35, Shinjuku-ku, Tokyo 160-8582, Japan.
authors: Hidaka
authors: H
authors: Kono
authors: Ken
authors: K
authors: Nakagawa
authors: Shinya
authors: S
authors: Morita
authors: Kazunobu
authors: K
authors: Shinoda
authors: Ryuichi
authors: R
authors: Mizuno
authors: Eiji
authors: E
authors: Kikuchi
authors: Akira
authors: A
authors: Miyajima
authors: Kazuo
authors: K
authors: Umezawa
authors: Mototsugu
authors: M
authors: Oya
mesh_terms: D000818:Animals; D051799:Delayed Graft Function / Q000188:drug therapy* / Q000209:etiology; D004195:Disease Models, Animal; D006085:Graft Survival / Q000187:drug effects*; D020672:I-kappa B Proteins / Q000494:pharmacology*; D016030:Kidney Transplantation; D000072000:NF-KappaB Inhibitor alpha; D051381:Rats; D011917:Rats, Inbred Lew; D015427:Reperfusion Injury / Q000150:complications / Q000188:drug therapy*; D016896:Treatment Outcome
chemical_list: D020672:I-kappa B Proteins; C000606206:Nfkbia protein, rat; D000072000:NF-KappaB Inhibitor alpha