title: Effects of salinity on photoreactivation of Escherichia coli after UV disinfection.
abstract: The effects of sodium chloride on photoreactivation of Escherichia coli were examined, assuming the discharge of ultraviolet (UV)-treated wastewater to water environment at different salinities. Suspensions of E. coli were first exposed to a low-pressure UV lamp in phosphate buffer to achieve 3 log inactivation, followed by an exposure to fluorescent light in NaCl solutions at the concentration of 1.0, 1.4, 1.9, 2.4 and 2.9 weight/volume %. When photoreactivation was completed in 3 h, survival ratio was recovered about 2 log in 1.0, 1.4, and 1.9% NaCl solutions, which was equivalent to the recovery observed in phosphate-buffered solution. Meanwhile, the recovery was suppressed to 0.8 log and -0.2 log in 2.4 and 2.9% NaCl solutions, respectively, which was significantly less than the recovery in phosphate buffer according to the t-test (p < 0.05). An endonuclease sensitive site assay demonstrated that the suppressed photoreactivation in 2.9% NaCl solution was due to the failure at repairing UV-induced pyrimidine dimers in the genome. In conclusion, photoreactivation of E. coli was significantly suppressed in NaCl solution at 2.4% or higher but not affected in NaCl solution at 1.9% or lower. This implies that photoreactivation of E. coli may potentially occur in brackish and coastal areas where salinity is rather low.
authors: Department of Urban Engineering, The University of Tokyo, Tokyo, Japan. oguma@env.t.u-tokyo.ac.jp
authors: Kumiko
authors: K
authors: Oguma
authors: Kentaro
authors: K
authors: Izaki
authors: Hiroyuki
authors: H
authors: Katayama
mesh_terms: D004203:Disinfection / Q000379:methods*; D004926:Escherichia coli / Q000528:radiation effects*; D054712:Salinity; D014466:Ultraviolet Rays; D062065:Wastewater / Q000382:microbiology*; D018508:Water Purification / Q000379:methods*
chemical_list: D062065:Wastewater