title: Molecular demonstration of hemotropic mycoplasmas in wild Japanese monkeys (Macaca fuscata).
abstract: The prevalence of hemotropic mycoplasmas in wild monkeys is largely unknown. Here, we report the presence of hemoplasmas in blood specimens collected from wild Japanese monkeys (Macaca fuscata) tentatively captured for ecological survey in Mie prefecture, Japan. We examined 9 monkeys using hemoplasma-specific real-time PCR and found all of them positive for a hemoplasma infection. The 16S rRNA gene and 16S to 23S rRNA intergenic spacer region of the hemoplasma detected in wild monkeys were amplified using end-point PCR. The nucleotide sequences of the PCR products were further determined and compared to those of other hemoplasmas. Our examinations revealed a wide prevalence of a hemoplasma strain in Japanese monkeys, which was similar to 'Candidatus Mycoplasma haemomacaque' reported in cynomolgus monkeys (Macaca fascicularis). Pathogenic traits of this hemoplasma strain remain unexplored.
authors: Department of Veterinary Medicine, Faculty of Agriculture, Iwate University, Morioka 020-8550, Japan.
authors: Hinako
authors: H
authors: Sashida
authors: Yoshihisa
authors: Y
authors: Suzuki
authors: Sou
authors: S
authors: Rokuhara
authors: Kazuya
authors: K
authors: Nagai
authors: Ryô
authors: R
authors: Harasawa
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000835:Animals, Wild; D001483:Base Sequence; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D007564:Japan / Q000453:epidemiology; D008251:Macaca; D008969:Molecular Sequence Data; D008992:Monkey Diseases / Q000453:epidemiology / Q000821:virology*; D009174:Mycoplasma / Q000235:genetics*; D009175:Mycoplasma Infections / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000737:chemistry / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
chemical_list: D004279:DNA, Viral; D012336:RNA, Ribosomal, 16S
references: J Mol Evol. 1980 Dec;16(2):111-20
references: 7463489
references: Microbiol Immunol. 1999;43(2):127-32
references: 10229266
references: Mol Biol Evol. 1987 Jul;4(4):406-25
references: 3447015
references: Microbes Infect. 2002 Jun;4(7):693-8
references: 12067828
references: Vet Microbiol. 2013 Apr 12;163(1-2):184-9
references: 23313325
references: Vet Clin Pathol. 2004;33(1):2-13
references: 15048620
references: J Clin Microbiol. 2013 May;51(5):1408-11
references: 23408694
references: J Clin Microbiol. 2005 Sep;43(9):4311-5
references: 16145070
references: Microbiol Immunol. 2005;49(9):859-63
references: 16172541
references: Ann Trop Med Parasitol. 1974 Mar;68(1):47-50
references: 4210496
references: Vet Pathol. 1994 May;31(3):301-7
references: 8053124
references: Int J Syst Evol Microbiol. 2000 May;50 Pt 3:1325-1329
references: 10843078
references: Int J Syst Evol Microbiol. 2001 May;51(Pt 3):891-899
references: 11411711
references: Int J Syst Evol Microbiol. 2002 Mar;52(Pt 2):683
references: 11931184
references: Vet Microbiol. 2011 May 5;149(3-4):478-81
references: 21145673
references: Clin Infect Dis. 2011 Dec;53(11):e147-51
references: 22021921
references: J Parasitol. 1972 Jun;58(3):628-30
references: 4625325
references: FEBS Lett. 1992 Feb 10;297(3):209-11
references: 1371968
references: Nucleic Acids Res. 1994 Nov 11;22(22):4673-80
references: 7984417
references: Microbiology (Reading). 2004 Apr;150(Pt 4):1023-1029
references: 15073311