title: Autologous dental pulp stem cells in regeneration of defect created in canine periodontal tissue.
abstract: This study aimed to investigate effects of dental pulp stem cells (DPSCs) on regeneration of a defect experimentally created in the periodontium of a canine model. Surgically created mesial 3-walled periodontal defects with ligature-induced periodontitis were produced bilaterally in the first lower premolar teeth of 10 mongrel dogs. Simultaneously, DPSCs were derived from the maxillary premolar teeth of the same dogs. Four weeks after creation of the periodontitis model, autologous passaged-3 DPSCs combined with Bio-Oss were implanted on one side as the test group. On the other side, only Bio-Oss was implanted as a control. Eight weeks after surgery, regeneration of the periodontal defects was evaluated histologically and histomorphometrically in terms of bone, periodontal ligament (PDL), and cement formation. Histologically, in all test specimens (10 defects), regeneration of cementum, bone, and PDL was observed. In the control groups, although we observed the regeneration of bone in all defects, the formation of cementum was seen in 9 defects and PDL was seen in 8 defects. Histomorphometric analyses showed that the amount of regenerated cementum and PDL in the test groups (3.83 ± 1.32 mm and 3.30 ± 1.12 mm, respectively) was significantly higher than that of the control groups (2.42 ± 1.40 mm and 1.77 ± 1.27 mm, respectively; P < .05). A biocomplex consisting of DPSCs and Bio-Oss would be promising in regeneration of periodontal tissues.
authors: Dental Research Center, Periodontics Department, Faculty of Dentistry, Tehran University of Medical Sciences, Tehran, Iran.
authors: Afshin
authors: A
authors: Khorsand
authors: Mohamadreza Baghaban
authors: MB
authors: Eslaminejad
authors: Mohadeseh
authors: M
authors: Arabsolghar
authors: Mojghan
authors: M
authors: Paknejad
authors: Baharak
authors: B
authors: Ghaedi
authors: Amir Reza
authors: AR
authors: Rokn
authors: Neda
authors: N
authors: Moslemi
authors: Hamid
authors: H
authors: Nazarian
authors: Shahrbanoo
authors: S
authors: Jahangir
mesh_terms: D050156:Adipogenesis; D016301:Alveolar Bone Loss / Q000601:surgery*; D000818:Animals; D001861:Bone Regeneration; D018786:Bone Substitutes; D002478:Cells, Cultured; D020219:Chondrogenesis; D003739:Dental Cementum / Q000502:physiology; D003782:Dental Pulp / Q000166:cytology*; D004285:Dogs; D008297:Male; D008903:Minerals; D010513:Periodontal Ligament / Q000502:physiology; D010518:Periodontitis / Q000601:surgery*; D012038:Regeneration; D033581:Stem Cell Transplantation; D054457:Tissue Scaffolds
chemical_list: C077540:Bio-Oss; D018786:Bone Substitutes; D008903:Minerals