title: Expansion of multipotent stem cells from the adult human brain.
abstract: The discovery of stem cells in the adult human brain has revealed new possible scenarios for treatment of the sick or injured brain. Both clinical use of and preclinical research on human adult neural stem cells have, however, been seriously hampered by the fact that it has been impossible to passage these cells more than a very few times and with little expansion of cell numbers. Having explored a number of alternative culturing conditions we here present an efficient method for the establishment and propagation of human brain stem cells from whatever brain tissue samples we have tried. We describe virtually unlimited expansion of an authentic stem cell phenotype. Pluripotency proteins Sox2 and Oct4 are expressed without artificial induction. For the first time multipotency of adult human brain-derived stem cells is demonstrated beyond tissue boundaries. We characterize these cells in detail in vitro including microarray and proteomic approaches. Whilst clarification of these cells' behavior is ongoing, results so far portend well for the future repair of tissues by transplantation of an adult patient's own-derived stem cells.
authors: Vilhelm Magnus Laboratory for Neurosurgical Research, Institute for Surgical Research, Oslo University Hospital, Oslo, Norway. wayne.murrell@rr-research.no
authors: Wayne
authors: W
authors: Murrell
authors: Emily
authors: E
authors: Palmero
authors: John
authors: J
authors: Bianco
authors: Biljana
authors: B
authors: Stangeland
authors: Mrinal
authors: M
authors: Joel
authors: Linda
authors: L
authors: Paulson
authors: Bernd
authors: B
authors: Thiede
authors: Zanina
authors: Z
authors: Grieg
authors: Ingunn
authors: I
authors: Ramsnes
authors: Håvard K
authors: HK
authors: Skjellegrind
authors: Ståle
authors: S
authors: Nygård
authors: Petter
authors: P
authors: Brandal
authors: Cecilie
authors: C
authors: Sandberg
authors: Einar
authors: E
authors: Vik-Mo
authors: Sheryl
authors: S
authors: Palmero
authors: Iver A
authors: IA
authors: Langmoen
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000378:metabolism; D001921:Brain / Q000166:cytology*; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation; D002999:Clone Cells / Q000166:cytology; D059290:Dopaminergic Neurons / Q000166:cytology; D005786:Gene Expression Regulation; D006801:Humans; D007621:Karyotyping; D008875:Middle Aged; D039902:Multipotent Stem Cells / Q000166:cytology* / Q000378:metabolism; D010641:Phenotype; D040901:Proteomics; D013194:Staining and Labeling
chemical_list: D015415:Biomarkers
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