title: ABC transporters in Dictyostelium discoideum development.
abstract: ATP-binding cassette (ABC) transporters can translocate a broad spectrum of molecules across the cell membrane including physiological cargo and toxins. ABC transporters are known for the role they play in resistance towards anticancer agents in chemotherapy of cancer patients. There are 68 ABC transporters annotated in the genome of the social amoeba Dictyostelium discoideum. We have characterized more than half of these ABC transporters through a systematic study of mutations in their genes. We have analyzed morphological and transcriptional phenotypes for these mutants during growth and development and found that most of the mutants exhibited rather subtle phenotypes. A few of the genes may share physiological functions, as reflected in their transcriptional phenotypes. Since most of the abc-transporter mutants showed subtle morphological phenotypes, we utilized these transcriptional phenotypes to identify genes that are important for development by looking for transcripts whose abundance was unperturbed in most of the mutants. We found a set of 668 genes that includes many validated D. discoideum developmental genes. We have also found that abcG6 and abcG18 may have potential roles in intercellular signaling during terminal differentiation of spores and stalks.
authors: Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas, United States of America.
authors: Edward Roshan
authors: ER
authors: Miranda
authors: Olga
authors: O
authors: Zhuchenko
authors: Marko
authors: M
authors: Toplak
authors: Balaji
authors: B
authors: Santhanam
authors: Blaz
authors: B
authors: Zupan
authors: Adam
authors: A
authors: Kuspa
authors: Gad
authors: G
authors: Shaulsky
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000235:genetics* / Q000378:metabolism*; D002454:Cell Differentiation / Q000235:genetics; D004023:Dictyostelium / Q000166:cytology / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism*; D009154:Mutation; D010641:Phenotype; D015800:Protozoan Proteins / Q000235:genetics* / Q000378:metabolism*; D033761:Spores, Protozoan / Q000166:cytology / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D014158:Transcription, Genetic
chemical_list: D018528:ATP-Binding Cassette Transporters; D015800:Protozoan Proteins
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