title: Expression analysis of chlorophyllid α binding protein, a secretory, red fluorescence protein in the midgut of silkworm, Bombyx mori.
abstract: Chlorophyllid α binding protein (chbp) was recently characterized by its ability to bind the prosthetic group of chlorophylls and little information is known regarding its expression. In the present study, we found that chpb was expressed highly and exclusively in the midgut of silkworm, Bombyx mori. The expression level of chbp was very high in the newly molted fifth instar larvae followed by gradual decline in the same instar. Our results demonstrated that CHBP was a secretory protein and located mainly in the apical of midgut epithelial cells. Real-time polymerase chain reaction analysis results showed that chpb highly expressed in the anterior midgut, threefold and sixfold higher compared with that of the middle midgut and posterior midgut, respectively, and chpb expression declined in darkness. In addition, the expression of chbp was affected by high-dose virus or bacterium infection.
authors: Institute of Sericulture and Apiculture, College of Animal Sciences, Zhejiang University, Hangzhou, China.
authors: Lin
authors: L
authors: Chen
authors: Rui
authors: R
authors: Yang
authors: Xiao-Long
authors: XL
authors: Hu
authors: Xing-Wei
authors: XW
authors: Xiang
authors: Xiao-Feng
authors: XF
authors: Wu
mesh_terms: D000818:Animals; D012831:Bombyx / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D002352:Carrier Proteins; D002735:Chlorophyllides / Q000378:metabolism*; D004064:Digestive System / Q000737:chemistry / Q000378:metabolism; D005786:Gene Expression Regulation; D018507:Gene Expression Regulation, Developmental; D019476:Insect Proteins / Q000235:genetics* / Q000378:metabolism*; D007814:Larva / Q000235:genetics / Q000378:metabolism; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism*; D009928:Organ Specificity; D011485:Protein Binding; D021381:Protein Transport; D000097573:Red Fluorescent Protein
chemical_list: D002352:Carrier Proteins; D002735:Chlorophyllides; D019476:Insect Proteins; D008164:Luminescent Proteins; D000097573:Red Fluorescent Protein; C034388:chlorophyllide a
keywords: Bombyx mori; chlorophyllid α binding protein; expression; midgut