title: Mos1 transposon-based transformation of fish cell lines using baculoviral vectors.
abstract: Drosophila Mos1 belongs to the mariner family of transposons, which are one of the most ubiquitous transposons among eukaryotes. We first determined nuclear transportation of the Drosophila Mos1-EGFP fusion protein in fish cell lines because it is required for a function of transposons. We next constructed recombinant baculoviral vectors harboring the Drosophila Mos1 transposon or marker genes located between Mos1 inverted repeats. The infectivity of the recombinant virus to fish cells was assessed by monitoring the expression of a fluorescent protein encoded in the viral genome. We detected transgene expression in CHSE-214, HINAE, and EPC cells, but not in GF or RTG-2 cells. In the co-infection assay of the Mos1-expressing virus and reporter gene-expressing virus, we successfully transformed CHSE-214 and HINAE cells. These results suggest that the combination of a baculovirus and Mos1 transposable element may be a tool for transgenesis in fish cells.
authors: Laboratory of Applied Molecular Entomology, Division of Applied Bioscience, Research Faculty of Agriculture, Hokkaido University, Sapporo 060-8589, Japan.
authors: Masako
authors: M
authors: Yokoo
authors: Ryosuke
authors: R
authors: Fujita
authors: Yumiko
authors: Y
authors: Nakajima
authors: Mamoru
authors: M
authors: Yoshimizu
authors: Hisae
authors: H
authors: Kasai
authors: Shin-ichiro
authors: S
authors: Asano
authors: Hisanori
authors: H
authors: Bando
mesh_terms: D000818:Animals; D016367:Baculoviridae / Q000235:genetics*; D002461:Cell Line, Transformed; D002467:Cell Nucleus / Q000378:metabolism; D004251:DNA Transposable Elements / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000235:genetics*; D004330:Drosophila; D005432:Flounder; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins / Q000378:metabolism; D020071:Interspersed Repetitive Sequences; D018613:Microscopy, Confocal; D017686:Oncorhynchus mykiss; D010957:Plasmids / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism; D012474:Salmon; D014162:Transfection; D019895:Transposases / Q000235:genetics*
chemical_list: D004251:DNA Transposable Elements; D004268:DNA-Binding Proteins; D011994:Recombinant Proteins; C111652:mariner transposases; D049452:Green Fluorescent Proteins; D019895:Transposases
keywords: Baculovirus; Fish cells; Mariner transposon; Mos1