title: Characterization and bioactivity of polysaccharides obtained from pine cones of Pinus koraiensis by graded ethanol precipitation.
abstract: Pinus koraiensis polysaccharides (PKP) were extracted by hot water from P. koraiensis pine cones. Five polysaccharide fractions named PKP-A, PKP-B, PKP-C, PKP-D and PKP-E were successfully separated at final ethanol concentrations of 30%, 50%, 60%, 70% and 80%, respectively. HPLC, FT-IR, GC-MS and automatic amino-acid analysis were applied to investigate their chemical characteristics. Monosaccharide component analysis indicated that the five fractions were all composed of D-ribose, L-rhamnose, L-arabinose, D-xylose, D-mannose, D-glucose and D-galactose, but their molar ratios were quite different. HPLC results revealed that the polysaccharides precipitated by higher concentrations of ethanol solution had lower molecular masses. Moreover, the antioxidant activities of the five fractions were studied on the basis of hydroxyl radical and ABTS radical scavenging tests. The five graded polysaccharide fractions exhibited good inhibitory power, and MTT tests in vitro showed the IC50 of PKP-A and PKP-E were 1,072.5 and 2,070.0 μg · mL-1, respectively. These results demonstrated that the PKP could be a potential source of natural antioxidants or dietary supplements.
authors: School of Food Science and Engineering, Harbin Institute of Technology, Harbin 150090, Helongjiang, China. zoupan0601@163.com
authors: Pan
authors: P
authors: Zou
authors: Xin
authors: X
authors: Yang
authors: Wei-Wei
authors: WW
authors: Huang
authors: Hai-Tian
authors: HT
authors: Zhao
authors: Jing
authors: J
authors: Wang
authors: Ren-Bo
authors: RB
authors: Xu
authors: Xing-Long
authors: XL
authors: Hu
authors: Si-Yan
authors: SY
authors: Shen
authors: Di
authors: D
authors: Qin
mesh_terms: D001089:Arabinose / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D000431:Ethanol / Q000737:chemistry*; D005690:Galactose / Q000737:chemistry; D008401:Gas Chromatography-Mass Spectrometry; D005947:Glucose / Q000737:chemistry; D008358:Mannose / Q000737:chemistry; D028223:Pinus / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry*; D012266:Ribose / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared; D014994:Xylose / Q000737:chemistry
chemical_list: D011134:Polysaccharides; D000431:Ethanol; D012266:Ribose; D014994:Xylose; D001089:Arabinose; D005947:Glucose; D008358:Mannose; D005690:Galactose
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