title: Detection of epigenetic alterations using tiling arrays.
abstract: The epigenetic status of cancer cells is a consequence of the neoplastic transformation of their normal counterpart. Epigenetic changes directly influence gene expression and chromatin organization, which consequently leads to escape from the tumor-suppression mechanisms. Global mapping for specific epigenetic modifications (e.g., DNA methylation) of the entire genome is required to reveal epigenetic hotspots associated with a cancer type/stage. DNA tiling arrays may be applied for genome-wide analysis of different epigenetic marks. Tiling arrays are high-density DNA microarrays that can be custom-made to survey regions of interest (e.g., gene promoters) or permit whole-genome analysis. To identify the genomic alterations associated with testicular cancers we used tiling arrays to profile their methylome. We successfully identified numerous epigenetically modified loci that arose as a consequence of tumor progression.
authors: Laboratory of Clinical and Developmental Genomics, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD, USA.
authors: Hoi-Hung
authors: HH
authors: Cheung
authors: Owen M
authors: OM
authors: Rennert
authors: Tin-Lap
authors: TL
authors: Lee
mesh_terms: D000818:Animals; D004247:DNA / Q000032:analysis / Q000235:genetics*; D019175:DNA Methylation; D015342:DNA Probes / Q000032:analysis / Q000235:genetics; D044127:Epigenesis, Genetic; D015972:Gene Expression Regulation, Neoplastic; D016678:Genome; D023281:Genomics / Q000295:instrumentation / Q000379:methods; D006801:Humans; D009369:Neoplasms / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation / Q000379:methods*
chemical_list: D015342:DNA Probes; D004247:DNA