title: Chemical, enzymatic and cellular antioxidant activity studies of Agaricus blazei Murrill.
abstract: Mushrooms possess nutritional and medicinal properties that have long been used for human health preservation and that have been considered by researchers as possible sources of free radical scavengers. In this work, the antioxidant properties of water extracts from Agaricus blazei Murill, produced by maceration and decoction, are demonstrated in vitro. Resistance to oxidation is demonstrated through three mechanisms: i) inhibition of enzymatic oxidative process, with 100% inhibition of HRP (horseradish peroxidase) and MPO (myeloperoxidase); ii) inhibition of cellular oxidative stress, with 80% inhibition of the oxidative burst of polymorphonuclear neutrophils (PMNs); and iii) direct action over reactive species, with 62% and 87% suppression of HOCl and superoxide anion radical (O2• -), respectively. From the data, it was concluded that the aqueous extract of A. blazei has significant antioxidant activity, indicating its possible application for nutraceutical and medicinal purposes.
authors: Universidade Estadual Paulista, Júlio de Mesquita Filho, Departamento de Bioquímica e Tecnologia Química, Instituto de Química, 14800-900 Araraquara, SP, Brasil.
authors: Ricardo A
authors: RA
authors: Hakime-Silva
authors: José C R
authors: JC
authors: Vellosa
authors: Najeh M
authors: NM
authors: Khalil
authors: Omar A K
authors: OA
authors: Khalil
authors: Iguatemy L
authors: IL
authors: Brunetti
authors: Olga M M F
authors: OM
authors: Oliveira
mesh_terms: D000364:Agaricus / Q000737:chemistry* / Q000201:enzymology; D000975:Antioxidants / Q000302:isolation & purification / Q000494:pharmacology*; D016166:Free Radical Scavengers; D049449:Luminescence; D009504:Neutrophils / Q000201:enzymology*; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D009195:Peroxidase / Q000378:metabolism; D013997:Time Factors
chemical_list: D000975:Antioxidants; D016166:Free Radical Scavengers; D009195:Peroxidase