title: Investigating gene promoter methylation in a mouse model of status epilepticus.
abstract: Epigenetic modification of DNA by methylation of the cytosine present in CG dinucleotides constitutes a key regulatory mechanism in the control of gene expression in neurological diseases. In this chapter, we describe an in-depth methodology of methylated DNA immunoprecipitation used in combination with tiling microarrays (MeDIP-chip) in order to analyze genome-wide gene promoter methylation in the hippocampus of mice following status epilepticus (prolonged seizure). While a specific mouse model and array format are described, the method can be applied to DNA from many tissues to analyze the methylation status of promoter regions across whole genomes, using a wide range of available array formats (both custom designed and commercially catalogued). We conclude the chapter with the description of bisulfite sequencing validation of MeDIP-chip results.
authors: Department of Physiology and Medical Physics, The Royal College of Surgeons in Ireland, Dublin, Ireland.
authors: Suzanne F C
authors: SF
authors: Miller-Delaney
authors: Sudipto
authors: S
authors: Das
authors: Raymond L
authors: RL
authors: Stallings
authors: David C
authors: DC
authors: Henshall
mesh_terms: D000818:Animals; D004247:DNA / Q000032:analysis / Q000235:genetics*; D019175:DNA Methylation; D004195:Disease Models, Animal; D047468:Immunoprecipitation / Q000379:methods; D051379:Mice; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D011401:Promoter Regions, Genetic; D013226:Status Epilepticus / Q000235:genetics*; D013447:Sulfites / Q000737:chemistry
chemical_list: D013447:Sulfites; D004247:DNA; C042345:hydrogen sulfite