title: Modulation of lipocalin-type prostaglandin D2 synthase expression in catfish seminal vesicles by thyroid disrupting agents and hormones.
abstract: Thyroid hormones play crucial role in several biological processes including reproduction. Disruption of normal thyroid status by environmental contaminants can cause severe impairment in reproductive functions. In our previous study, we reported down-regulation of a protein in seminal vesicular fluid of air-breathing catfish, Clarias gariepinus during experimentally induced hyperthyroidism. N-terminal amino acid sequence analysis followed by search in sequence database denoted it to be lipocalin-type prostaglandin D2 synthase (ptgds-b). In the present study, we cloned full-length cDNA of ptgds-b based on the N-terminal amino acid sequence. Surprisingly, Northern blot as well as RT-PCR analysis demonstrated the presence of ptgds-b transcript predominantly in seminal vesicles and developing testis. Further, ptgds-b mRNA significantly decreased in seminal vesicles following L-thyroxine overdose while there was an increased expression of ptgds-b after depletion of thyroid hormone by thiourea and withdrawal of the treatments reverted this effect. Treatment of catfish with human chorionic gonadotropin and estradiol significantly reduced ptgds-b expression. Taken together, we report ptgds-b as a thyroid hormone regulated protein in the seminal vesicles in addition to gonadotropin and estradiol. Further studies might explain the exclusive presence of ptgds-b in seminal vesicles and developing testis yet present data evaluated it as a putative biomarker for thyroid hormone disruption.
authors: Department of Animal Sciences, School of Life Sciences - Centre for Advanced Studies, University of Hyderabad, P.O. Central University, Hyderabad 500 046, India.
authors: Gunti
authors: G
authors: Sreenivasulu
authors: Ayinampudi
authors: A
authors: Pavani
authors: Cheni-Chery
authors: CC
authors: Sudhakumari
authors: Aparna
authors: A
authors: Dutta-Gupta
authors: Balasubramanian
authors: B
authors: Senthilkumaran
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D006063:Chorionic Gonadotropin / Q000494:pharmacology; D004958:Estradiol / Q000494:pharmacology; D005260:Female; D029941:Fish Proteins / Q000235:genetics*; D018507:Gene Expression Regulation, Developmental; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D006801:Humans; D019746:Intramolecular Oxidoreductases / Q000145:classification / Q000235:genetics*; D054834:Lipocalins / Q000145:classification / Q000235:genetics*; D008297:Male; D008969:Molecular Sequence Data; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012669:Seminal Vesicles / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D013737:Testis / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism; D013890:Thiourea / Q000494:pharmacology; D013961:Thyroid Gland / Q000187:drug effects / Q000378:metabolism; D013974:Thyroxine / Q000494:pharmacology*; D059467:Transcriptome / Q000187:drug effects*
chemical_list: D006063:Chorionic Gonadotropin; D029941:Fish Proteins; D054834:Lipocalins; D004958:Estradiol; D019746:Intramolecular Oxidoreductases; C022466:prostaglandin R2 D-isomerase; D013890:Thiourea; D013974:Thyroxine
keywords: Catfish; Endocrine disruption; Seminal vesicles; Thiourea; Thyroxine; ptgds-b