title: GSK3β inhibition and LEF1 upregulation in skeletal muscle following a bout of downhill running.
abstract: Canonical Wnt signaling is important in skeletal muscle repair but has not been well characterized in response to physiological stimuli. The objective of this study was to assess the effect of downhill running (DHR) on components of Wnt signaling. Young, male C57BL/J6 mice were exposed to DHR. Muscle injury and repair (MCadherin) were measured in soleus. Gene and protein expression of Wnt3a, active β-catenin, GSK3β, and LEF1 were measured in gastrocnemius. Muscle injury increased 6 days post-DHR and MCadherin protein increased 5 days post-DHR. Total and active GSK3β protein decreased 3 days (9-fold and 3.6-fold, respectively) post-DHR. LEF1 protein increased 6 days (5-fold) post-DHR. DHR decreased GSK3β and increased LEF1 protein expression, but did not affect other components of Wnt signaling. Due to their applicability, using models of physiological stimuli such as DHR will provide significant insight into cellular mechanisms within muscle.
authors: Molecular Biology Core Facility, Cannon Research Center, Charlotte, NC, USA.
authors: Hiral
authors: H
authors: Amin
authors: Judy
authors: J
authors: Vachris
authors: Alicia
authors: A
authors: Hamilton
authors: Nury
authors: N
authors: Steuerwald
authors: Reuben
authors: R
authors: Howden
authors: Susan Tsivitse
authors: ST
authors: Arthur
mesh_terms: D000818:Animals; D015820:Cadherins / Q000378:metabolism; D015536:Down-Regulation / Q000502:physiology*; D038362:Glycogen Synthase Kinase 3 / Q000378:metabolism*; D000071679:Glycogen Synthase Kinase 3 beta; D051821:Lymphoid Enhancer-Binding Factor 1 / Q000378:metabolism*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D023421:Models, Animal; D018482:Muscle, Skeletal / Q000378:metabolism*; D010805:Physical Conditioning, Animal / Q000502:physiology*; D012420:Running / Q000502:physiology*; D013997:Time Factors; D015854:Up-Regulation / Q000502:physiology*; D060449:Wnt Signaling Pathway / Q000502:physiology; D060509:Wnt3A Protein / Q000378:metabolism; D051176:beta Catenin / Q000378:metabolism
chemical_list: D015820:Cadherins; C497574:Lef1 protein, mouse; D051821:Lymphoid Enhancer-Binding Factor 1; D060509:Wnt3A Protein; C555511:Wnt3a protein, mouse; D051176:beta Catenin; C070896:M-cadherin; D000071679:Glycogen Synthase Kinase 3 beta; C000605507:Gsk3b protein, mouse; D038362:Glycogen Synthase Kinase 3
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