title: Identification of the STAT1 gene and the characterisation of its immune response to immunostimulants, including nervous necrosis virus (NNV) infection, in Malabar grouper (Epinephelus malabaricus).
abstract: Signal Transducer and Activator of Transcription (STAT)-1 is an indispensable signal transduction protein that is involved in the interferon pathway. STAT-1 plays an important role in the innate immune response. The full-length cDNA of Malabar grouper (Epinephelus malabaricus) STAT-1, MgSTAT1, was cloned. Phylogenetic analysis was performed based on the amino acid sequence. Our results indicate that STAT1 is highly conserved with other vertebrates. We also report the expression of MgSTAT1 in different tissues treated with immune stimulants, including LPS, CpG ODN, and poly (I:C), in vivo. The expression of MgSTAT1 was significantly induced in the head kidney upon treatment with poly (I:C) compared to the control. Moreover, the results indicate that MgSTAT1 is up-regulated during nervous necrosis virus (NNV) infection. This study reveals that similar to the mammalian antiviral response, MgSTAT1 mediates the immune response in Malabar grouper.
authors: Department of Aquaculture, National Taiwan Ocean University, No. 2, Beining Rd., Keelung 20224, Taiwan.
authors: Chun-Hsi
authors: CH
authors: Tso
authors: Yu-Fang
authors: YF
authors: Hung
authors: Sook-Ping
authors: SP
authors: Tan
authors: Ming-Wei
authors: MW
authors: Lu
mesh_terms: D000276:Adjuvants, Immunologic / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D016000:Cluster Analysis; D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D005393:Fish Diseases / Q000235:genetics / Q000276:immunology* / Q000821:virology*; D005786:Gene Expression Regulation / Q000187:drug effects / Q000276:immunology*; D060226:Head Kidney / Q000378:metabolism; D008969:Molecular Sequence Data; D030381:Nodaviridae / Q000276:immunology*; D010473:Perciformes; D010802:Phylogeny; D011070:Poly I-C / Q000494:pharmacology; D012327:RNA Virus Infections / Q000235:genetics / Q000276:immunology / Q000662:veterinary*; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary; D050794:STAT1 Transcription Factor / Q000235:genetics / Q000378:metabolism*; D016415:Sequence Alignment / Q000662:veterinary; D017422:Sequence Analysis, DNA / Q000662:veterinary
chemical_list: D000276:Adjuvants, Immunologic; D017931:DNA Primers; D018076:DNA, Complementary; D050794:STAT1 Transcription Factor; D011070:Poly I-C
keywords: Epinephelus malabaricus; Immune response; Interferon; Nervous necrosis virus; STAT1