title: A chemically defined medium for rabbit embryo cryopreservation.
abstract: This study evaluates a new synthetic substitute (CRYO3, Ref. 5617, Stem Alpha, France) for animal-based products in rabbit embryo cryopreservation solutions. This evaluation was performed using two approaches: a thermodynamic approach using differential scanning calorimetry and a biological approach using rabbit embryo slow-freezing. During the experiment, foetal calf serum (FCS) was used as a reference. Because FCS varies widely by supplier, three different FCS were selected for the thermodynamic approach. The rabbit embryo slow-freezing solutions were made from Dulbecco's phosphate buffer saline containing 1.5 M Dimethyl Sulfoxide and 18% (v.v(-1)) of CRYO3 or 18% (v.v(-1)) of FCS. These solutions were evaluated using four characteristics: the end of melting temperature, the enthalpy of crystallisation (thermodynamic approach) and the embryo survival rates after culture and embryo transfer (biological approach). In the thermodynamic approach, the solutions containing one of the three different FCS had similar mean thermodynamic characteristics but had different variabilities in the overall data with aberrant values. The solution containing CRYO3 had similar thermodynamic properties when compared to those containing FCS. Moreover, no aberrant value was measured in the solution containing CRYO3. This solution appears to be more stable than the solutions containing a FCS. In the biological approach, the in vitro embryo survival rates obtained with the solution containing CRYO3 (73.7% and 81.3%) and with the solution containing a FCS (77.6% and 71.9%) were similar (p = 0.7). Nevertheless, during the in vivo evaluation, the implantation rate (21.8%) and the live-foetuses rate (18.8%) of the CRYO3 group were significantly higher than the implantation rate (7.1%, p = 0.0002) and the live-foetuses rate (5.3%, p = 0.0002) of the FCS group. The pregnancy rate was also higher in the CRYO3 group compared to the FCS group (81.3% and 43.8%, respectively, p = 0.066). We conclude that CRYO3 can be used as a chemically defined substitute for animal-based products in rabbit embryo cryopreservation solutions.
authors: UPSP 2011.03.10 (ICE), VetAgro Sup (Université de Lyon), Marcy l'étoile, France.
authors: Pierre
authors: P
authors: Bruyère
authors: Anne
authors: A
authors: Baudot
authors: Thierry
authors: T
authors: Joly
authors: Loris
authors: L
authors: Commin
authors: Elodie
authors: E
authors: Pillet
authors: Pierre
authors: P
authors: Guérin
authors: Gérard
authors: G
authors: Louis
authors: Anne
authors: A
authors: Josson-Schramme
authors: Samuel
authors: S
authors: Buff
mesh_terms: D000818:Animals; D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents / Q000494:pharmacology*; D004622:Embryo, Mammalian / Q000187:drug effects*; D005260:Female; D011247:Pregnancy; D011817:Rabbits; D044967:Serum; D012996:Solutions; D013816:Thermodynamics; D014022:Tissue Survival / Q000187:drug effects
chemical_list: D003451:Cryoprotective Agents; D012996:Solutions
references: J Pharm Sci. 2001 Jul;90(7):860-71
references: 11458335
references: Cryobiology. 2009 Apr;58(2):157-65
references: 19118541
references: Cryobiology. 2002 Dec;45(3):204-12
references: 12510005
references: Reprod Nutr Dev. 2001 Sep-Oct;41(5):439-50
references: 11993801
references: Vet J. 2000 Jan;159(1):10-7
references: 10640408
references: Biol Reprod. 2012 Oct 18;87(4):91
references: 22855563
references: J Exp Biol. 2006 Sep;209(Pt 17):3469-75
references: 16916982
references: Cryobiology. 1984 Apr;21(2):183-91
references: 6713947
references: Am J Vet Res. 1980 Oct;41(10):1680-1
references: 6261615
references: Theriogenology. 2008 Mar 15;69(5):612-23
references: 18242668
references: Hum Reprod. 2010 Feb;25(2):470-8
references: 19933523
references: Cryobiology. 2006 Feb;52(1):128-38
references: 16336957
references: Cryobiology. 2007 Apr;54(2):212-22
references: 17350609
references: Theriogenology. 2009 Oct 15;72(7):919-25
references: 19616294
references: Dev Biol Stand. 1991;75:177-81
references: 1665461
references: Blood. 2008 Dec 1;112(12):4739-45
references: 18647958
references: Theriogenology. 2007 Apr 1;67(6):1185-93
references: 17321587
references: Anim Reprod Sci. 1997 Aug;48(2-4):247-56
references: 9452877
references: Theriogenology. 1995 Oct;44(5):705-14
references: 16727768
references: Cryobiology. 2001 Aug;43(1):21-31
references: 11812048
references: Theriogenology. 2000 Mar 15;53(5):1157-66
references: 10798492
references: Theriogenology. 2009 Feb;71(3):499-508
references: 18834622
references: Reprod Domest Anim. 2006 Aug;41(4):260-7
references: 16869879
references: Biologicals. 2003 Jun;31(2):137-43
references: 12770546
references: Theriogenology. 2002 Nov;58(8):1541-52
references: 12374124
references: Theriogenology. 2008 Sep 15;70(5):725-45
references: 18586320
references: Cold Spring Harb Protoc. 2010 Jan;2010(1):pdb.emo139
references: 20150104
references: Mol Reprod Dev. 2006 Dec;73(12):1600-11
references: 16902954
references: Cryobiology. 2006 Oct;53(2):268-75
references: 16930582
references: Cryobiology. 2000 Mar;40(2):151-8
references: 10788314
references: Theriogenology. 1994;42(7):1205-15
references: 16727625
references: Lancet. 2004 Feb 7;363(9407):417-21
references: 14962520
references: Theriogenology. 2006 Sep 1;66(4):964-73
references: 16574210
references: J Exp Zool. 2001 Aug 1;290(3):299-310
references: 11479909
references: Mol Reprod Dev. 2007 Nov;74(11):1395-405
references: 17342731
references: Dev Biol Stand. 1991;75:173-5
references: 1665460
references: Cryobiology. 2000 Aug;41(1):35-42
references: 11017759
references: Life Sci. 1986 Nov 10;39(19):1751-3
references: 3773642
references: Theriogenology. 2008 Jan 1;69(1):129-36
references: 18028999
references: Anim Reprod Sci. 2005 Apr;86(3-4):329-38
references: 15766810