title: [DNA methylation status of miR-126 and its host gene EGFL7 in CD4+ T cells from patients with systemic lupus erythematosus].
abstract: OBJECTIVE
  To explore the mechanisms by which DNA methylation regulates miR-126 and its host gene EGFL7 in CD4+ T cells from patients with systemic lupus erythematosus (SLE).
  
  
  METHODS
  We analyzed the expression and the DNA methylation status within promoter region of EGFL7 and miR-126 by real-time qPCR and bisulfite genomic sequencing analysis.
  
  
  RESULTS
  miR-126 and EGFL7 mRNA expression was upregulated in CD4+ T cells from SLE compared with that from healthy controls (P<0.01). EGFL7 mRNA level was positively correlated with miR-126 expression in CD4+ T cells from SLE (r=0.538, P=0.015). The average methylation level of EGFL7 promoter in CD4+ T cells from SLE was lower than that from healthy controls (P<0.05).
  
  
  CONCLUSIONS
  The upregulation of miR-126 and its host gene EGFL7 expression in CD4+ T cells from SLE is associated with the hypomethylation of the EGFL7 promoter.
authors: Hunan Key Laboratory of Medical Epigenomics, Changsha 410078;Department of Dermatology, Second Xiangya Hospital, Central South University, Changsha 410011,China.
authors: Yunsheng
authors: Y
authors: Liang
authors: Sha
authors: S
authors: Zhao
authors: Gongping
authors: G
authors: Liang
authors: Ming
authors: M
authors: Zhao
authors: Qianjin
authors: Q
authors: Lu
mesh_terms: D000293:Adolescent; D000328:Adult; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology / Q000378:metabolism*; D002135:Calcium-Binding Proteins; D019175:DNA Methylation; D066255:EGF Family of Proteins; D016228:Endothelial Growth Factors / Q000235:genetics / Q000378:metabolism*; D005260:Female; D006801:Humans; D008180:Lupus Erythematosus, Systemic / Q000235:genetics* / Q000276:immunology; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D055815:Young Adult
chemical_list: D002135:Calcium-Binding Proteins; D066255:EGF Family of Proteins; C527877:EGFL7 protein, human; D016228:Endothelial Growth Factors; C527111:MIRN126 microRNA, human; D035683:MicroRNAs; D012333:RNA, Messenger