title: Tonotopic organization of vertical cells in the dorsal cochlear nucleus of the CBA/J mouse.
abstract: The systematic and topographic representation of frequency is a first principle of organization throughout the auditory system. The dorsal cochlear nucleus (DCN) receives direct tonotopic projections from the auditory nerve (AN) as well as secondary and descending projections from other sources. Among the recipients of AN input in the DCN are vertical cells (also called tuberculoventral cells), glycinergic interneurons thought to provide on- or near-best-frequency feed-forward inhibition to principal cells in the DCN and various cells in the anteroventral cochlear nucleus (AVCN). Differing lines of physiological and anatomical evidence suggest that vertical cells and their projections are organized with respect to frequency, but this has not been conclusively demonstrated in the intact mammalian brain. To address this issue, we retrogradely labeled vertical cells via physiologically targeted injections in the AVCN of the CBA/J mouse. Results from multiple cases were merged with a normalized 3D template of the cochlear nucleus (Muniak et al. [2013] J. Comp. Neurol. 521:1510-1532) to demonstrate quantitatively that the arrangement of vertical cells is tonotopic and aligned to the innervation pattern of the AN. These results suggest that vertical cells are well positioned for providing immediate, frequency-specific inhibition onto cells of the DCN and AVCN to facilitate spectral processing.
authors: Department of Neuroscience, Johns Hopkins University, Baltimore, Maryland, 21205; Hearing Research Program, Garvan Institute of Medical Research, Sydney, New South Wales, 2010, Australia.
authors: Michael A
authors: MA
authors: Muniak
authors: David K
authors: DK
authors: Ryugo
mesh_terms: D000200:Action Potentials / Q000502:physiology; D000818:Animals; D001306:Auditory Pathways / Q000502:physiology; D001931:Brain Mapping; D003056:Cochlear Nerve / Q000502:physiology; D017626:Cochlear Nucleus / Q000166:cytology*; D005260:Female; D005452:Fluoresceins / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D005998:Glycine; D021621:Imaging, Three-Dimensional; D007395:Interneurons / Q000502:physiology*; D008297:Male; D051379:Mice; D008808:Mice, Inbred CBA; D018408:Patch-Clamp Techniques; D012044:Regression Analysis
chemical_list: D005452:Fluoresceins; D005998:Glycine
keywords: 3D reconstruction; auditory nerve; frequency organization; interneuron; tuberculoventral; type II
references: J Neurophysiol. 1999 Aug;82(2):648-63
references: 10444663
references: J Neurophysiol. 1995 Feb;73(2):743-65
references: 7760132
references: J Neurophysiol. 2012 Feb;107(3):824-35
references: 22072506
references: Anat Rec A Discov Mol Cell Evol Biol. 2006 Apr;288(4):397-408
references: 16550586
references: J Acoust Soc Am. 1996 Sep;100(3):1691-702
references: 8817895
references: J Comp Neurol. 1996 Jan 29;365(1):159-72
references: 8821448
references: J Assoc Res Otolaryngol. 2011 Oct;12(5):633-45
references: 21717290
references: J Comp Neurol. 2004 Sep 20;477(3):253-72
references: 15305363
references: J Neurophysiol. 2012 Aug;108(4):1186-98
references: 22572947
references: J Neurosci. 2001 Oct 1;21(19):7848-58
references: 11567076
references: Hear Res. 1991 Jan;51(1):11-28
references: 1672865
references: Neurosci Lett. 1988 Jan 11;84(1):13-7
references: 2831482
references: J Comp Neurol. 1985 May 22;235(4):529-53
references: 3998222
references: J Neurophysiol. 1990 Nov;64(5):1590-610
references: 2283542
references: Am J Anat. 1982 Feb;163(2):103-30
references: 7072613
references: J Comp Neurol. 1972 Mar;144(3):355-72
references: 5027335
references: J Neurophysiol. 1994 Jun;71(6):2446-62
references: 7931527
references: Neuroscience. 1987 Jan;20(1):209-19
references: 3561761
references: J Neurosci. 2002 Dec 15;22(24):10891-7
references: 12486183
references: J Neurophysiol. 1976 Mar;39(2):282-300
references: 1255224
references: Hear Res. 1996 Apr;93(1-2):167-80
references: 8735077
references: J Neurophysiol. 1996 Apr;75(4):1411-31
references: 8727387
references: Brain Res. 1987 Jan 1;400(1):145-50
references: 2434184
references: Brain Res. 1980 Oct 27;200(1):23-37
references: 7417810
references: Hear Res. 1981 Jul;4(3-4):215-41
references: 7263511
references: J Comp Neurol. 1984 Nov 1;229(3):432-50
references: 6209306
references: J Comp Neurol. 1993 Feb 15;328(3):377-92
references: 7680052
references: J Comp Neurol. 1993 Mar 1;329(1):36-52
references: 8454725
references: J Comp Neurol. 2013 May 1;521(7):1510-32
references: 23047723
references: Hear Res. 1982 Feb;6(2):153-69
references: 7061349
references: J Comp Neurol. 2005 Apr 4;484(2):191-205
references: 15736230
references: Brain Res. 1996 Jan 8;706(1):97-102
references: 8720496
references: J Comp Neurol. 2001 Jul 30;436(3):290-303
references: 11438931
references: J Comp Neurol. 1989 May 8;283(2):228-47
references: 2738197
references: J Neurosci. 2013 Jan 23;33(4):1598-614
references: 23345233
references: J Comp Neurol. 1969 Aug;136(4):453-84
references: 5801446
references: Exp Brain Res. 1973 Jun 29;17(4):402-27
references: 4725899
references: Brain Res. 1981 Apr 6;210(1-2):342-9
references: 6164444
references: J Comp Neurol. 2007 Oct 10;504(5):583-98
references: 17701985
references: Hear Res. 1992 Mar;58(2):132-52
references: 1568936
references: J Comp Neurol. 1993 Mar 1;329(1):20-35
references: 8454724
references: J Basic Clin Physiol Pharmacol. 1996;7(3):199-220
references: 8910137
references: J Neurophysiol. 1994 Nov;72(5):2124-33
references: 7884448
references: Brain Res. 1987 Jul 7;415(1):183-7
references: 3304530
references: J Comp Neurol. 1994 Jan 15;339(3):311-27
references: 8132865
references: J Neurophysiol. 1993 May;69(5):1409-21
references: 8389823
references: J Neurophysiol. 1980 Jul;44(1):76-96
references: 7420140
references: J Neurophysiol. 2000 Feb;83(2):926-40
references: 10669505
references: J Acoust Soc Am. 1974 Mar;55(3):620-30
references: 4819862
references: J Neurosci. 1990 Jun;10(6):1762-8
references: 1972392
references: J Neurophysiol. 1999 Aug;82(2):1019-32
references: 10444694
references: J Comp Neurol. 2008 Sep 20;510(3):297-308
references: 18634002
references: J Comp Neurol. 1988 Feb 15;268(3):389-99
references: 3360996
references: J Comp Neurol. 1974 Jun 1;155(3):251-300
references: 4134212