title: Burkholderia humi sp. nov., Burkholderia choica sp. nov., Burkholderia telluris sp. nov., Burkholderia terrestris sp. nov. and Burkholderia udeis sp. nov.: Burkholderia glathei-like bacteria from soil and rhizosphere soil.
abstract: Analysis of partial gyrB gene sequences revealed six taxa in a group of 17 Burkholderia glathei-like isolates which were further examined by (GTG)5-PCR fingerprinting, 16S rRNA gene sequence analysis, DNA-DNA hybridizations, determination of the DNA G+C content, whole-cell fatty acid analysis and an analysis of cell and colony morphology and more than 180 biochemical characteristics. The results demonstrated that one taxon consisting of three human clinical isolates represented Burkholderia zhejiangensis, a recently described methyl-parathion-degrading bacterium isolated from a wastewater-treatment system in China. The remaining taxa represented five novel species isolated from soil or rhizosphere soil samples, and could be distinguished by both genotypic and phenotypic characteristics. We therefore propose to formally classify these bacteria as Burkholderia humi sp. nov. (type strain, LMG 22934(T) = CCUG 63059(T)), Burkholderia choica sp. nov. (type strain, LMG 22940(T) = CCUG 63063(T)), Burkholderia telluris sp. nov. (type strain, LMG 22936(T) = CCUG 63060(T)), Burkholderia udeis sp. nov. (type strain, LMG 27134(T) = CCUG 63061(T)) and Burkholderia terrestris sp. nov. (type strain, LMG 22937(T) = CCUG 63062(T)).
authors: Laboratorium voor Microbiologie, Vakgroep Biochemie en microbiologie, Faculteit Wetenschappen, Universiteit Gent, Gent, Belgium.
authors: Peter
authors: P
authors: Vandamme
authors: Laboratorium voor Microbiologie, Vakgroep Biochemie en microbiologie, Faculteit Wetenschappen, Universiteit Gent, Gent, Belgium.
authors: Evie
authors: E
authors: De Brandt
authors: Vakgroep Veterinaire Volksgezondheid en Voedselveiligheid, Faculteit Diergeneeskunde, Universiteit Gent, Gent, Belgium.
authors: Kurt
authors: K
authors: Houf
authors: Department of Microbial Ecology, Center for Life Sciences, University of Groningen, The Netherlands.
authors: Joana Falcão
authors: JF
authors: Salles
authors: Department of Microbial Ecology, Center for Life Sciences, University of Groningen, The Netherlands.
authors: Jan
authors: J
authors: Dirk van Elsas
authors: Department of Pediatrics and Communicable Disease, University of Michigan Medical School, Ann Arbor, MI 48109, USA.
authors: Theodore
authors: T
authors: Spilker
authors: Department of Pediatrics and Communicable Disease, University of Michigan Medical School, Ann Arbor, MI 48109, USA.
authors: John J
authors: JJ
authors: LiPuma
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D019117:Burkholderia / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D002681:China; D027081:DNA Gyrase / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D006801:Humans; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D058441:Rhizosphere; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D062065:Wastewater / Q000382:microbiology
chemical_list: D027081:DNA Gyrase; D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D062065:Wastewater