title: Site-specific characterization of the Asp- and Glu-ADP-ribosylated proteome.
abstract: Poly(ADP-ribosyl)ation is catalyzed by a family of enzymes known as PARPs. We describe a method to characterize the human aspartic acid- and glutamic acid-ADP-ribosylated proteome. We identified 1,048 ADP-ribosylation sites on 340 proteins involved in a wide array of nuclear functions; among these were many previously unknown PARP downstream targets whose ADP-ribosylation was sensitive to PARP inhibitor treatment. We also confirmed that iniparib had a negligible effect on PARP activity in intact cells.
authors: Department of Biochemistry, University of Texas Southwestern Medical Center, Dallas, Texas, USA.
authors: Yajie
authors: Y
authors: Zhang
authors: Jianqi
authors: J
authors: Wang
authors: Ming
authors: M
authors: Ding
authors: Yonghao
authors: Y
authors: Yu
mesh_terms: D000246:Adenosine Diphosphate Ribose / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D001224:Aspartic Acid / Q000737:chemistry* / Q000378:metabolism; D001665:Binding Sites; D004249:DNA Damage; D055785:Gene Knockdown Techniques; D018698:Glutamic Acid / Q000737:chemistry* / Q000378:metabolism; D006026:Glycoside Hydrolases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D045325:HCT116 Cells; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D008958:Models, Molecular; D000071137:Poly (ADP-Ribose) Polymerase-1; D000067856:Poly(ADP-ribose) Polymerase Inhibitors; D011065:Poly(ADP-ribose) Polymerases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D011487:Protein Conformation; D011499:Protein Processing, Post-Translational; D020543:Proteome / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism
chemical_list: D000067856:Poly(ADP-ribose) Polymerase Inhibitors; D020543:Proteome; D000246:Adenosine Diphosphate Ribose; D001224:Aspartic Acid; D018698:Glutamic Acid; D006861:Hydrogen Peroxide; C499320:PARP1 protein, human; D000071137:Poly (ADP-Ribose) Polymerase-1; D011065:Poly(ADP-ribose) Polymerases; D006026:Glycoside Hydrolases
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