title: The effects of 30 days resveratrol supplementation on adipose tissue morphology and gene expression patterns in obese men.
abstract: Polyphenolic compounds, such as resveratrol, have recently received widespread interest because of their ability to mimic effects of calorie restriction. The objective of the present study was to gain more insight into the effects of 30 days resveratrol supplementation on adipose tissue morphology and underlying processes. Eleven healthy obese men were supplemented with placebo and resveratrol for 30 days (150 mg per day), separated by a 4-week washout period in a double-blind randomized crossover design. A postprandial abdominal subcutaneous adipose tissue biopsy was collected to assess adipose tissue morphology and gene expression using microarray analysis. Resveratrol significantly decreased adipocyte size, with a shift toward a reduction in the proportion of large and very-large adipocytes and an increase in small adipocytes. Microarray analysis revealed downregulation of Wnt and Notch signaling pathways and upregulation of pathways involved in cell cycle regulation after resveratrol supplementation, suggesting enhanced adipogenesis. Furthermore, lysosomal/phagosomal pathway and transcription factor EB were upregulated reflecting an alternative pathway of lipid breakdown by autophagy. Further research is necessary to investigate whether resveratrol improves adipose tissue function.
authors: Department of Human Biology, NUTRIM School for Nutrition, Toxicology and Metabolism, Maastricht University Medical Center+, Maastricht, The Netherlands.
authors: E
authors: E
authors: Konings
authors: 1] Department of Human Biology, NUTRIM School for Nutrition, Toxicology and Metabolism, Maastricht University Medical Center+, Maastricht, The Netherlands [2] Top Institute Food and Nutrition (TIFN), Wageningen, The Netherlands.
authors: S
authors: S
authors: Timmers
authors: 1] Nutrition, Metabolism and Genomics group, Division of Human Nutrition, Wageningen University, Wageningen, The Netherlands [2] Netherlands Nutrigenomics Centre, TI Food and Nutrition, Wageningen, The Netherlands.
authors: M V
authors: MV
authors: Boekschoten
authors: Department of Human Biology, NUTRIM School for Nutrition, Toxicology and Metabolism, Maastricht University Medical Center+, Maastricht, The Netherlands.
authors: G H
authors: GH
authors: Goossens
authors: Department of Human Biology, NUTRIM School for Nutrition, Toxicology and Metabolism, Maastricht University Medical Center+, Maastricht, The Netherlands.
authors: J W
authors: JW
authors: Jocken
authors: 1] Nutrition, Metabolism and Genomics group, Division of Human Nutrition, Wageningen University, Wageningen, The Netherlands [2] Netherlands Nutrigenomics Centre, TI Food and Nutrition, Wageningen, The Netherlands.
authors: L A
authors: LA
authors: Afman
authors: 1] Nutrition, Metabolism and Genomics group, Division of Human Nutrition, Wageningen University, Wageningen, The Netherlands [2] Netherlands Nutrigenomics Centre, TI Food and Nutrition, Wageningen, The Netherlands.
authors: M
authors: M
authors: Müller
authors: 1] Department of Human Biology, NUTRIM School for Nutrition, Toxicology and Metabolism, Maastricht University Medical Center+, Maastricht, The Netherlands [2] Top Institute Food and Nutrition (TIFN), Wageningen, The Netherlands.
authors: P
authors: P
authors: Schrauwen
authors: Department of Human Biology, NUTRIM School for Nutrition, Toxicology and Metabolism, Maastricht University Medical Center+, Maastricht, The Netherlands.
authors: E C
authors: EC
authors: Mariman
authors: Department of Human Biology, NUTRIM School for Nutrition, Toxicology and Metabolism, Maastricht University Medical Center+, Maastricht, The Netherlands.
authors: E E
authors: EE
authors: Blaak
mesh_terms: D017667:Adipocytes / Q000187:drug effects* / Q000378:metabolism*; D050156:Adipogenesis / Q000187:drug effects; D000273:Adipose Tissue / Q000187:drug effects / Q000378:metabolism*; D000328:Adult; D000368:Aged; D018592:Cross-Over Studies; D004311:Double-Blind Method; D004791:Enzyme Inhibitors / Q000627:therapeutic use*; D020869:Gene Expression Profiling; D006801:Humans; D008297:Male; D008875:Middle Aged; D009765:Obesity / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism; D051880:Receptors, Notch / Q000187:drug effects / Q000378:metabolism; D000077185:Resveratrol; D015398:Signal Transduction / Q000187:drug effects; D013267:Stilbenes / Q000627:therapeutic use*; D016896:Treatment Outcome; D060449:Wnt Signaling Pathway / Q000187:drug effects
chemical_list: D004791:Enzyme Inhibitors; D051880:Receptors, Notch; D013267:Stilbenes; D000077185:Resveratrol
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