title: The prospective isolation of viable, high ploidy megakaryocytes from adult murine bone marrow by fluorescence activated cell sorting.
abstract: Mature megakaryocytes (MM) can be up to 65 μM in diameter and due to their size, viable and pure MM populations have been difficult to isolate in large numbers. Here in, we report a fluorescence activated cell sorting (FACS) method by which viable and pure populations of 8 N, 16 N, 32 N, and 64 N MM can be isolated from murine bone marrow (BM).
authors: Materials Science and Engineering, Commonwealth Scientific and Industrial Research Organization, Melbourne, VIC, Australia.
authors: Shen Y
authors: SY
authors: Heazlewood
authors: Brenda
authors: B
authors: Williams
authors: Melonie J
authors: MJ
authors: Storan
authors: Susan K
authors: SK
authors: Nilsson
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000502:physiology*; D002470:Cell Survival; D002478:Cells, Cultured; D005434:Flow Cytometry; D018189:Immunomagnetic Separation; D008533:Megakaryocytes / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D040201:Platelet Membrane Glycoprotein IIb / Q000378:metabolism; D011123:Polyploidy
chemical_list: D040201:Platelet Membrane Glycoprotein IIb