title: Transpositional transgenesis withpiggyBac.
abstract: Transposons are mobile genetic elements that are capable of self-directed excision and subsequent reintegration within the host genome. Transposase such aspiggyBac, Sleeping BeautyandTol2catalyze these reactions and have shown potential as tools for the stable integration of transgenes when used in the binary plasmid mode. Recent modifications to the transposase and/or the terminal repeats of the transposon have increased their integration efficiency and/or specificity. We recently described the development of apiggyBactransposase system, the helper independent, single construct self-inactivating plasmid called GENIE. Here we describe the structure, safety and function of these transpositional vectors and their use in animal transgenesis and cell transfection.
authors: Department of Anatomy, Biochemistry and Physiology; John A. Burns School of Medicine; Honolulu, HI USA.
authors: Johann
authors: J
authors: Urschitz
authors: Stefan
authors: S
authors: Moisyadi
keywords: cell transfections; piggyBac; transgene; transgenesis; transposase; transposon
references: Proc Natl Acad Sci U S A. 2010 May 4;107(18):8117-22
references: 20404201
references: Transl Res. 2013 Apr;161(4):265-83
references: 23313630
references: BMC Biotechnol. 2007 Jan 18;7:5
references: 17233894
references: Reprod Nutr Dev. 2001 Nov-Dec;41(6):465-85
references: 12126294
references: Science. 2002 Feb 1;295(5556):868-72
references: 11786607
references: Proc Natl Acad Sci U S A. 2008 Jul 8;105(27):9290-5
references: 18579772
references: Cell. 1997 Nov 14;91(4):501-10
references: 9390559
references: Cell. 2005 Aug 12;122(3):473-83
references: 16096065
references: Science. 2010 Nov 19;330(6007):1104-7
references: 20947725
references: Hum Gene Ther. 2007 Dec;18(12):1193-204
references: 17988194
references: J Androl. 2012 Mar-Apr;33(2):229-38
references: 21546611
references: Physiol Genomics. 2007 Oct 22;31(2):159-73
references: 17684037
references: Curr Gene Ther. 2005 Aug;5(4):367-73
references: 16101511
references: Biol Reprod. 2003 May;68(5):1903-10
references: 12606337
references: Nucleic Acids Res. 2007;35(12):e87
references: 17576687
references: J Bacteriol. 1997 Sep;179(18):5827-34
references: 9294441
references: Stem Cells Int. 2011;2011:717069
references: 21977042
references: Methods Cell Biol. 2004;74:219-42
references: 15575609
references: Nucleic Acids Res. 2011 Dec;39(22):e148
references: 21948799
references: Science. 1999 May 14;284(5417):1180-3
references: 10325231
references: Proc Natl Acad Sci U S A. 1980 Dec;77(12):7380-4
references: 6261253
references: Proc Natl Acad Sci U S A. 2012 Nov 20;109(47):19184-9
references: 23093669
references: Proc Natl Acad Sci U S A. 2006 Oct 10;103(41):15008-13
references: 17005721
references: Genome Biol. 2007;8 Suppl 1:S12
references: 18047689