title: Role of motor cortex NMDA receptors in learning-dependent synaptic plasticity of behaving mice.
abstract: The primary motor cortex has an important role in the precise execution of learned motor responses. During motor learning, synaptic efficacy between sensory and primary motor cortical neurons is enhanced, possibly involving long-term potentiation and N-methyl-D-aspartate (NMDA)-specific glutamate receptor function. To investigate whether NMDA receptor in the primary motor cortex can act as a coincidence detector for activity-dependent changes in synaptic strength and associative learning, here we generate mice with deletion of the Grin1 gene, encoding the essential NMDA receptor subunit 1 (GluN1), specifically in the primary motor cortex. The loss of NMDA receptor function impairs primary motor cortex long-term potentiation in vivo. Importantly, it impairs the synaptic efficacy between the primary somatosensory and primary motor cortices and significantly reduces classically conditioned eyeblink responses. Furthermore, compared with wild-type littermates, mice lacking NMDA receptors in the [corrected] primary motor cortex show slower learning in Skinner-box tasks. Thus, primary motor cortex NMDA receptors are necessary for activity-dependent synaptic strengthening and associative learning.
authors: 1] Max Planck Institute for Medical Research, 69120 Heidelberg, Germany [2] Charité-Universitätsmedizin, NeuroCure Cluster of Excellence, 12101 Berlin, Germany.
authors: Mazahir T
authors: MT
authors: Hasan
authors: Samuel
authors: S
authors: Hernández-González
authors: Godwin
authors: G
authors: Dogbevia
authors: Mario
authors: M
authors: Treviño
authors: Ilaria
authors: I
authors: Bertocchi
authors: Agnès
authors: A
authors: Gruart
authors: José M
authors: JM
authors: Delgado-García
mesh_terms: D000818:Animals; D001522:Behavior, Animal / Q000502:physiology*; D003216:Conditioning, Operant / Q000502:physiology; D055724:Electrophysiological Phenomena; D017353:Gene Deletion; D055786:Gene Knockout Techniques; D019426:Integrases / Q000378:metabolism; D007858:Learning; D017774:Long-Term Potentiation / Q000502:physiology; D051379:Mice; D009044:Motor Cortex / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D009473:Neuronal Plasticity / Q000502:physiology*; D009928:Organ Specificity; D016194:Receptors, N-Methyl-D-Aspartate / Q000235:genetics / Q000378:metabolism*; D013569:Synapses / Q000378:metabolism*; D013647:Task Performance and Analysis
chemical_list: C121673:Gprin1 protein, mouse; D009419:Nerve Tissue Proteins; D016194:Receptors, N-Methyl-D-Aspartate; C045073:Cre recombinase; D019426:Integrases
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