title: Protection of human erythrocyte using Crinum asiaticum extract and lycorine from oxidative damage induced by 2-amidinopropane.
abstract: The intention of this investigation was to evaluate the free radical scavenging activity and erythrocyte protective activity of ethanolic extract of Crinum asiaticum (L) and lycorine. The ethanolic extract of C. asiaticum (L) and lycorine were found to have different levels of antioxidant properties in the test models. Both ethanolic extract of C. asiaticum (L) (0.5-2.5 mg/ml) and lycorine (0.010 mg-0.050 mg/ml) increases the percentage of lipid peroxidation inhibition (26.25 ± 0.23% and 19.25 ± 0.23%) and enhances the free radical scavenging activity (20.92 ± 0.22% and 20.52 ± 0.22%), scavenging of hydrogen peroxide (25.67 ± 0.17% and 23.07 ± 0.3%) superoxide anion scavenging activity (27.69 ± 0.16% and 16.09 ± 0.7%) at concentration of 2.5 and 0.050 mg of C. asiaticum (L) and lycorine, respectively. But compared with tocopherol (P < 0.05) less activity was observed by C. asiaticum (L) and lycorine. The ethanolic extract of C. asiaticum (L) and lycorine were normalized to reduce the level of glutathione and also to sustain the status of protein in erythrocytes during the peroxyl radical [2,2-azobis (2-amidinopropane) dihydrochloride (AAPH)] induced oxidative damage in ex vivo model. The present results of the investigations demonstrated that protective nature of the C. asiaticum (L) and lycorine will be considered as a significant natural antioxidant source.
authors: Department of Biotechnology, PRIST University, Thanjavur, Tamilnadu, India.
authors: S
authors: S
authors: Ilavenil
authors: B
authors: B
authors: Kaleeswaran
authors: P
authors: P
authors: Sumitha
authors: D
authors: D
authors: Tamilvendan
authors: S
authors: S
authors: Ravikumar
keywords: AAPH; Antioxidant; Crinumasiaticum (L); Free radical lipid peroxidation; Lycorine
references: Planta Med. 2002 May;68(5):454-7
references: 12058326
references: Carcinogenesis. 1989 Jun;10(6):1003-8
references: 2470525
references: Phytochem Anal. 2002 Jan-Feb;13(1):8-17
references: 11899609
references: Ann N Y Acad Sci. 1999 Nov 28;884:19-40
references: 10842581
references: FEBS Lett. 2004 Dec 17;578(3):245-50
references: 15589827
references: Methods Enzymol. 1994;234:279-93
references: 7808295
references: Biochim Biophys Acta. 1976 Mar 17;425(3):342-8
references: 944052
references: J Clin Invest. 1983 May;71(5):1215-23
references: 6853709
references: Antiviral Res. 2005 Jul;67(1):18-23
references: 15885816
references: Annu Rev Biochem. 1983;52:711-60
references: 6137189
references: Arzneimittelforschung. 1995 Mar;45(3A):443-6
references: 7763342
references: Methods Enzymol. 1984;105:328-31
references: 6727672
references: J Clin Invest. 1985 Nov;76(5):1971-7
references: 4056060
references: Life Sci. 2001 May 25;69(1):75-86
references: 11411807
references: J Biol Chem. 1951 Nov;193(1):265-75
references: 14907713
references: Life Sci. 1997;60(10):763-71
references: 9064481
references: Biochem Pharmacol. 1998 Mar 15;55(6):785-91
references: 9586950
references: Biochem Pharmacol. 1985 Dec 15;34(24):4283-9
references: 4074388
references: Free Radic Biol Med. 1989;6(3):289-301
references: 2663662
references: Planta Med. 2004 Mar;70(3):260-2
references: 15114506
references: J Agric Food Chem. 2005 Aug 24;53(17):6572-80
references: 16104768
references: Am J Med. 1991 Sep 30;91(3C):14S-22S
references: 1928205
references: Arch Biochem Biophys. 1963 Jan;100:119-30
references: 14028302
references: Free Radic Biol Med. 1992;12(6):487-98
references: 1601324
references: Food Chem Toxicol. 2006 Sep;44(9):1513-21
references: 16765500
references: Biol Pharm Bull. 2006 Sep;29(9):1906-10
references: 16946507
references: Biochem J. 1995 Apr 1;307 ( Pt 1):57-62
references: 7717994