title: Combination of small molecule microarray and confocal microscopy techniques for live cell staining fluorescent dye discovery.
abstract: Discovering new fluorochromes is significantly advanced by high-throughput screening (HTS) methods. In the present study a combination of small molecule microarray (SMM) prescreening and confocal laser scanning microscopy (CLSM) was developed in order to discover novel cell staining fluorescent dyes. Compounds with high native fluorescence were selected from a 14,585-member library and further tested on living cells under the microscope. Eleven compartment-specific, cell-permeable (or plasma membrane-targeted) fluorochromes were identified. Their cytotoxicity was tested and found that between 1-10 micromolar range, they were non-toxic even during long-term incubations.
authors: Laboratory of Functional Genomics, Institute of Genetics, Biological Research Center, Hungarian Academy of Sciences, Temesvári krt. 62, Szeged H-6726, Hungary. laszlo@avicorbiotech.com.
authors: Eszter
authors: E
authors: Molnár
authors: Soujanya
authors: S
authors: Kuntam
authors: Pradeep Kumar Reddy
authors: PK
authors: Cingaram
authors: Begüm
authors: B
authors: Peksel
authors: Bhavyashree
authors: B
authors: Suresh
authors: Gabriella
authors: G
authors: Fábián
authors: Liliána Z
authors: LZ
authors: Fehér
authors: Attila
authors: A
authors: Bokros
authors: Agnes
authors: A
authors: Medgyesi
authors: Ferhan
authors: F
authors: Ayaydin
authors: László G
authors: LG
authors: Puskás
mesh_terms: D005456:Fluorescent Dyes / Q000737:chemistry*; D018613:Microscopy, Confocal / Q000379:methods*; D015394:Molecular Structure
chemical_list: D005456:Fluorescent Dyes
references: J Med Chem. 2008 Apr 24;51(8):2363-71
references: 18363325
references: J Mol Recognit. 2010 Mar-Apr;23(2):209-19
references: 19746440
references: J Am Chem Soc. 2010 Dec 1;132(47):17015-22
references: 21062056
references: Chem Commun (Camb). 2009 Sep 7;(33):5030-2
references: 19668839
references: Photochem Photobiol Sci. 2007 Aug;6(8):848-52
references: 17668114
references: Nature. 2008 Apr 3;452(7187):580-9
references: 18385732
references: Chem Biol. 2006 Jun;13(6):615-23
references: 16793519
references: Lipids Health Dis. 2010 Jun 02;9:56
references: 20525221
references: Expert Opin Drug Discov. 2011 Dec;6(12):1299-315
references: 22647068
references: Expert Opin Drug Discov. 2011 Jan;6(1):17-32
references: 22328899
references: Mol Divers. 2003;7(1):25-36
references: 14768901
references: J Cell Sci. 2000 Sep;113 ( Pt 17):2941-53
references: 10934034
references: FEBS Lett. 2005 Feb 7;579(4):927-32
references: 15680976
references: Proc Natl Acad Sci U S A. 2012 Jun 26;109(26):10214-7
references: 22689954
references: Angew Chem Int Ed Engl. 2003 May 30;42(21):2376-9
references: 12783500
references: Curr Med Chem. 2004 Dec;11(23):3119-45
references: 15579004
references: J Nucl Med. 2008 Sep;49(9):1401-4
references: 18703594
references: Angew Chem Int Ed Engl. 2007;46(42):7960-4
references: 17868168
references: Science. 2006 Apr 14;312(5771):217-24
references: 16614209
references: Mol Biosyst. 2011 Feb;7(2):304-10
references: 21079875
references: Acta Histochem. 2011 May;113(3):363-8
references: 20138336