title: [Quantitative analysis of foodborne salmonella-the study of mini-modified semi solid rappaport vassiliadis most probable number method].
abstract: OBJECTIVE
  To improve the mini-modified semi solid rappaport vassiliadis most probable number (mini-MSRV MPN) method for Salmonella detection.
  
  
  METHODS
  Based on the mini-MSRV MPN method,Buffered Peptone Water (BPW) was modified as one step enrichment medium and Modified Semi Solid Rappaport Vassiliadis (MSRV) medium was ameliorated as modified MSRV for Salmonella detection under standard Salmonella addition recovery. A total of 154 raw chicken samples, 48 swabs of pheasantry and 48 poultry dung samples were collected to compare the detection results of Salmonella by using improved mini-MSRV MPN, mini-MSRV MPN and regular most probable number (MPN) method.
  
  
  RESULTS
  Salmonella recovery was < 2.7 MPN/g when the standard Salmonella addition was at the concentration of 0.9 CFU/g when the mini-MSRV MPN method was employed. If the standard Salmonella addition were at 9.0 and 90.0 CFU/g, the recoveries of bacteria were 10.1 and 94.0 MPN/g, and the average recovery rate was 112% and 104%, respectively. Salmonella detection rate of modified mini-MSRV MPN, mini-MSRV MPN and regular MPN method was 18.4% (46/250), 5.2% (13/250) and 6.0% (15/250), respectively. The detection rate was higher for modified mini-MSRV MPN method than of the other two methods (χ(2) values were 19.68 and 17.82, respectively, all P values < 0.05). The detection quantity of Salmonella (medians were 21.0, < 2.7 and < 3.0 MPN/g, respectively). The quantity detected by modified mini-MSRV MPN method was higher than that of the other two methods (both Z values were 5.71, both P values < 0.05).
  
  
  CONCLUSIONS
  Modified mini-MSRV MPN method is an accurate method for foodborne Salmonella detection.
authors: Henan Center for Disease Control and Prevention, Zhengzhou, China. zhangd222@hncdc.com.cn
authors: Ding
authors: D
authors: Zhang
authors: Xing-guang
authors: XG
authors: Liao
authors: Yun-chang
authors: YC
authors: Guo
authors: Xiu-li
authors: XL
authors: Zhang
authors: Hui-xia
authors: HX
authors: Chuan
authors: Ying
authors: Y
authors: Cui
mesh_terms: D000818:Animals; D002645:Chickens / Q000382:microbiology*; D003470:Culture Media; D005506:Food Contamination / Q000032:analysis*; D005513:Food Inspection / Q000379:methods*; D012475:Salmonella / Q000302:isolation & purification*
chemical_list: D003470:Culture Media