title: Coarse architecture of the transient receptor potential vanilloid 1 (TRPV1) ion channel determined by fluorescence resonance energy transfer.
abstract: The transient receptor potential vanilloid 1 ion channel is responsible for the perception of high temperatures and low extracellular pH, and it is also involved in the response to some pungent compounds. Importantly, it is also associated with the perception of pain and noxious stimuli. Here, we attempt to discern the molecular organization and location of the N and C termini of the transient receptor potential vanilloid 1 ion channel by measuring FRET between genetically attached enhanced yellow and cyan fluorescent protein to the N or C terminus of the channel protein, expressed in transfected HEK 293 cells or Xenopus laevis oocytes. The static measurements of the domain organization were mapped into an available cryo-electron microscopy density of the channel with good agreement. These measurements also provide novel insights into the organization of terminal domains and their proximity to the plasma membrane.
authors: From the Departamento de Fisiología, Facultad de Medicina, and.
authors: Víctor
authors: V
authors: De-la-Rosa
authors: Gisela E
authors: GE
authors: Rangel-Yescas
authors: Ernesto
authors: E
authors: Ladrón-de-Guevara
authors: Tamara
authors: T
authors: Rosenbaum
authors: León D
authors: LD
authors: Islas
mesh_terms: D000465:Algorithms; D000818:Animals; D002462:Cell Membrane / Q000737:chemistry / Q000502:physiology* / Q000648:ultrastructure; D020285:Cryoelectron Microscopy; D005260:Female; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D057809:HEK293 Cells; D006801:Humans; D015640:Ion Channel Gating / Q000502:physiology*; D007700:Kinetics; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D008564:Membrane Potentials / Q000502:physiology; D008958:Models, Molecular; D009865:Oocytes / Q000378:metabolism / Q000502:physiology; D018408:Patch-Clamp Techniques; D055503:Protein Multimerization; D017434:Protein Structure, Tertiary; D051381:Rats; D050916:TRPV Cation Channels / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D014982:Xenopus laevis
chemical_list: D008164:Luminescent Proteins; D050916:TRPV Cation Channels; C493922:Trpv1 protein, rat
keywords: Imaging; Pain; Patch Clamp; Plasma Membrane; Spectroscopy; TRP Channels
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