title: Remarks on the validity of Myxobolus ampullicapsulatus and Myxobolus honghuensis (Myxozoa: Myxosporea) based on SSU rDNA sequences.
abstract: In the present study, we isolated three populations of Myxobolus ampullicapsulatus from the gills of crucian carp, Carassius auratus auratus, two from Yongchuan, Chongqing area and one from Poyang Lake, Jiangxi area, China, sequenced their complete small subunit ribosome RNA gene, analyzed their genetic distance and gene similarity, and explored their relationship based on Bayesian inference and maximum likelihood analyses of their small subunit ribosomal DNA. The results combined with their morphological characteristics suggest that M. ampullicapsulatus infecting the gills and pharynx of allogynogenetic gibel carp, Carassius auratus gibelio, should be Myxobolus honghuensis. This study highlights the importance of DNA sequence comparisons for distinguishing Myxobolus species and indicates that the intra-species identification for the two Myxobolus species mentioned in the present research should be less than ten variation sites. In morphology, M. honghuensis Liu et al. (2012) parasitic on the gills of C. auratus auratus (goldfish) was collected from Chongqing area, and its mature spore was 16.5-19.5 × 8.5-10.0 μm in size, polar capsule was 7.0-10.0 × 2.5-4.0 μm in size, and polar filament had 9-10 coils. M. honghuensis Liu et al. (2012) isolated from the pharynx of C. auratus gibelio was sampled in Hubei area, and its mature spore was 15.1-19.5 × 9.0-11.3 μm in size, polar capsule was 7.9-8.1 × 3.0-4.5 μm in size, and polar filament had 7-8 coils.
authors: Chongqing Key Laboratory of Animal Biology, College of Life Sciences, Chongqing Normal University, Chongqing, 400047, People's Republic of China, zhaoyuanjuncqnu@126.com.
authors: Y J
authors: YJ
authors: Zhao
authors: N N
authors: NN
authors: Li
authors: F H
authors: FH
authors: Tang
authors: J L
authors: JL
authors: Dong
mesh_terms: D000818:Animals; D002347:Carps / Q000469:parasitology; D002681:China; D016000:Cluster Analysis; D016054:DNA, Protozoan / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D020459:Genes, rRNA; D014644:Genetic Variation; D005880:Gills / Q000469:parasitology; D008969:Molecular Sequence Data; D055766:Myxobolus / Q000145:classification* / Q000166:cytology / Q000235:genetics / Q000302:isolation & purification*; D010802:Phylogeny; D016053:RNA, Protozoan / Q000235:genetics; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D016054:DNA, Protozoan; D004275:DNA, Ribosomal; D016053:RNA, Protozoan; D012337:RNA, Ribosomal, 18S
references: J Parasitol. 2004 Jun;90(3):584-93
references: 15270103
references: Dis Aquat Organ. 2006 Nov 21;73(1):49-61
references: 17240752
references: J Parasitol. 2004 Jun;90(3):618-22
references: 15270109
references: J Parasitol. 1998 Apr;84(2):371-4
references: 9576514
references: Mol Phylogenet Evol. 2009 Oct;53(1):81-93
references: 19477283
references: J Eukaryot Microbiol. 2001 Jul-Aug;48(4):395-413
references: 11456316
references: Mol Biol Evol. 2011 Oct;28(10):2731-9
references: 21546353
references: Parasitol Res. 2012 Apr;110(4):1331-6
references: 21912961
references: Dis Aquat Organ. 2004 Aug 9;60(2):105-8
references: 15460854
references: Dis Aquat Organ. 2006 Apr 6;69(2-3):259-63
references: 16724571
references: J Parasitol. 2008 Dec;94(6):1322-34
references: 19127969
references: Parasitol Res. 2012 Oct;111(4):1423-35
references: 22699967
references: Folia Parasitol (Praha). 2007 Nov;54(4):272-6
references: 18303768
references: Int J Parasitol. 2006 Dec;36(14):1521-34
references: 16904677
references: Parasitol Res. 2012 Sep;111(3):1287-94
references: 22592536
references: Dis Aquat Organ. 2011 Feb 22;93(3):257-60
references: 21516979
references: J Eukaryot Microbiol. 2006 Sep-Oct;53(5):364-73
references: 16968454
references: J Fish Dis. 2010 Jan;33(1):57-68
references: 19912459
references: Syst Parasitol. 2005 Jun;61(2):115-22
references: 15980965
references: Parasitol Res. 2012 Aug;111(2):819-26
references: 22476601
references: J Parasitol. 2008 Jun;94(3):737-42
references: 18605802
references: Folia Parasitol (Praha). 2006 Mar;53(1):1-36
references: 16696428
references: Syst Parasitol. 2005 May;61(1):1-46
references: 15928990
references: Bioinformatics. 2003 Aug 12;19(12):1572-4
references: 12912839
references: Dis Aquat Organ. 2004 Jan 28;58(1):35-40
references: 15038449
references: Parasitology. 2007 May;134(Pt 5):669-81
references: 17234042
references: Dis Aquat Organ. 2005 Sep 23;66(3):227-32
references: 16261938
references: Parasitol Res. 2011 Jul;109(1):81-91
references: 21188599