title: Photo-mediated internalization of nanocomplex for effective gene delivery to adipose tissue-derived stem cells.
abstract: To deliver efficiently osteogenic, chondrogenic or adipogenic induction genes, such as Runx2, SOX9 and C/EBP-α, to adipose tissue-derived stem cells (ADSCs), a photo-mediated nanocomplex internalization gene delivery system was designed using chlorin e6 as a photosensitizer (PS) and polyethyleneimine (PEI) as a gene delivery carrier. In this system, gene delivery efficacy was significantly increased in ADSCs by photo irradiation. The gene transfection efficiency of Runx2, SOX9 and C/EBP-α was increased by 8.6-, 6.7- and 9.3-fold, respectively, by applying 0.7J/cm(2) of irradiation. Osteogenic, chondrogenic and adipogenic differentiation was confirmed by differentiation-related markers and histological analysis. ADSCs transfected with Runx2, SOX9 and C/EBP-α genes via photo irradiation indicated enhanced differentiation in comparison to the non-irradiated cells. These findings demonstrate that photo-mediated internalization is a promising system for efficient gene delivery and differentiation in ADSCs.
authors: Department of Biotechnology, The Catholic University of Korea, 43-1 Yeokkok2-dong, Wonmi-gu, Bucheon-si, Gyeonggi-do, 420-743, Republic of Korea.
authors: Sin-jung
authors: SJ
authors: Park
authors: Kun
authors: K
authors: Na
mesh_terms: D050156:Adipogenesis / Q000235:genetics; D000273:Adipose Tissue / Q000166:cytology*; D053687:Adult Stem Cells / Q000166:cytology* / Q000378:metabolism* / Q000528:radiation effects; D022762:CCAAT-Enhancer-Binding Proteins / Q000235:genetics; D018929:Cell Culture Techniques / Q000379:methods; D002454:Cell Differentiation / Q000235:genetics / Q000528:radiation effects; D002470:Cell Survival / Q000528:radiation effects; D064987:Cell- and Tissue-Based Therapy / Q000379:methods; D002478:Cells, Cultured; D020219:Chondrogenesis / Q000235:genetics; D050659:Core Binding Factor Alpha 1 Subunit / Q000235:genetics; D018014:Gene Transfer Techniques; D049452:Green Fluorescent Proteins / Q000235:genetics; D006801:Humans; D049329:Nanostructures; D010012:Osteogenesis / Q000235:genetics; D010789:Phototherapy / Q000379:methods*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D055758:SOX9 Transcription Factor / Q000235:genetics; D014162:Transfection
chemical_list: D022762:CCAAT-Enhancer-Binding Proteins; C515848:CEBPA protein, human; D050659:Core Binding Factor Alpha 1 Subunit; D012333:RNA, Messenger; C493709:RUNX2 protein, human; D055758:SOX9 Transcription Factor; C527932:SOX9 protein, human; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins
keywords: Adipose tissue-derived stem cells; Chlorin e6; Differentiation; Photo-mediated internalization; Photosensitizer