title: Nuclear pore proteins regulate chromatin structure and transcriptional memory by a conserved mechanism.
abstract: Previous experience alters the rate of transcriptional induction of many genes in yeast and this phenomenon persists through several cell division cycles. This phenomenon is called epigenetic transcriptional memory. For the yeast gene INO1, transcriptional memory requires a physical interaction with the nuclear pore complex (NPC) and changes in the chromatin structure of the promoter. These changes lead to binding of a preinitiation form of RNA Polymerase II (RNAPII) to the INO1 promoter, bypassing the need to recruit RNAPII to the promoter during reactivation. In our recent study, we found that in human cells, hundreds of interferon-γ responsive genes exhibit a mechanistically similar form of transcriptional memory. Transcriptional memory requires a homologous nuclear pore protein in yeast and humans, which interacts with the promoters of genes that exhibit transcriptional memory and promotes both alteration of chromatin structure and binding of RNAPII. Whereas the interaction of yeast genes with nuclear pore proteins occurs at the NPC, the interaction of human genes with nuclear pore proteins occurs in the nucleoplasm. Thus, the interaction of nuclear pore proteins with genes plays an important and conserved role in affecting long-term epigenetic changes in transcriptional regulation.
authors: Department of Molecular Biosciences; Northwestern University; Evanston, IL USA.
authors: William H
authors: WH
authors: Light
authors: Jason H
authors: JH
authors: Brickner
mesh_terms: D002843:Chromatin / Q000235:genetics* / Q000378:metabolism*; D006801:Humans; D022022:Nuclear Pore / Q000378:metabolism*; D028861:Nuclear Pore Complex Proteins / Q000378:metabolism; D012319:RNA Polymerase II / Q000378:metabolism; D014158:Transcription, Genetic
chemical_list: D002843:Chromatin; D028861:Nuclear Pore Complex Proteins; C465451:Nup98 protein, human; D012319:RNA Polymerase II
keywords: chromatin; epigenetics; nuclear pore complex; nuclear porins; transcriptional memory
references: Proc Natl Acad Sci U S A. 2005 Apr 19;102(16):5749-54
references: 15817685
references: Cell. 2004 May 14;117(4):427-39
references: 15137937
references: J Cell Biol. 2005 Dec 19;171(6):955-65
references: 16365162
references: Mol Cell. 2010 Oct 8;40(1):112-25
references: 20932479
references: Genes Dev. 2004 Oct 15;18(20):2437-68
references: 15489290
references: Nat Cell Biol. 2010 Feb;12(2):111-8
references: 20098417
references: Cell. 2013 May 23;153(5):988-99
references: 23706737
references: Annu Rev Immunol. 1997;15:749-95
references: 9143706
references: Mol Cell Biol. 2010 Apr;30(8):2046-56
references: 20123968
references: Genes Dev. 2007 Apr 15;21(8):997-1004
references: 17438002
references: Genes Dev. 2005 May 15;19(10):1188-98
references: 15905407
references: Cell. 2002 May 31;109(5):551-62
references: 12062099
references: Curr Biol. 2002 Dec 23;12(24):2076-89
references: 12498682
references: Nature. 2009 Sep 10;461(7261):186-92
references: 19741698
references: Genes Dev. 2008 Mar 1;22(5):627-39
references: 18316479
references: Mol Cell. 2005 Apr 15;18(2):171-83
references: 15837421
references: Nature. 2007 Nov 29;450(7170):695-701
references: 18046406
references: Dev Cell. 2003 Jun;4(6):775-89
references: 12791264
references: Mol Cell. 2006 Aug 4;23(3):297-305
references: 16885020
references: Mol Biol Cell. 2012 Apr;23(7):1367-75
references: 22323286
references: Genes Dev. 2001 Jul 15;15(14):1771-82
references: 11459827
references: J Struct Biol. 2002 Oct-Dec;140(1-3):79-91
references: 12490156
references: Immunity. 2006 Mar;24(3):295-304
references: 16546098
references: PLoS Biol. 2013;11(3):e1001524
references: 23555195
references: J Leukoc Biol. 2004 Feb;75(2):163-89
references: 14525967
references: PLoS Biol. 2007 Apr;5(4):e81
references: 17373856
references: Mol Cell. 2006 Mar 17;21(6):811-23
references: 16543150
references: EMBO J. 2002 Nov 1;21(21):5843-52
references: 12411502
references: Cell. 1996 Jan 26;84(2):265-75
references: 8565072
references: Nature. 2000 Jan 6;403(6765):108-12
references: 10638763
references: Cell. 2010 Feb 5;140(3):372-83
references: 20144761
references: Cell. 2010 Feb 5;140(3):360-71
references: 20144760
references: J Cell Biol. 2001 Jun 25;153(7):1465-78
references: 11425876
references: Genetics. 2012 Oct;192(2):495-505
references: 22851651