title: Structural insights into the extra cellular segment of integrinβ5 and molecular interaction studies.
abstract: Primary tumor cells often spread to other organs by metastasis. Despite of it, primary tumor cells break their surrounding extra cellular matrix (ECM) proteins and reach the destination organ by the process of intravasation and extravasation. Metastasized tumor cells induce the process of angiogenesis, this highly regulated process involves several ECM proteins. However, integrins are primarily involved in the blood vessel growth and repair. Therefore, integrins are promising angiogenesis targets. Integrins are receptors on cell surface, involved in signal transduction and attachments in extra cellular matrix (ECM). IntegrinαVβ3 and αVβ5 are implicated in tumor angiogenesis, metastasis, inflammation and bone resorption. The crystal structure of integrinαvβ5 is not available in protein structural databases, therefore; molecular model of integrinβ5 structure was prepared and stereo chemical model quality was checked. Integrin β5 active sites were identified based on insilico analysis tools. Further, molecular level interactions between integrinβ5 and ECM proteins were predicted. In the present study ECM proteins such as focal adhesion kinase 1 (FAK1), annexin A5 and P21 activated kinase 4 (PAK4) were considered for protein-protein docking, to understand inter molecular interactions. The predicted model is conceived to be stereo chemically good and can be used for molecular interaction studies of angiogenic inhibitors.
authors: Depart of Genetics & Biotechnology, Osmania University, Hyderabad , Andhra Pradesh , India .
authors: Aravind
authors: A
authors: Setti
authors: Harsha Sagar
authors: HS
authors: Sankati
authors: T A Phazna
authors: TA
authors: Devi
authors: A Chandra
authors: AC
authors: Sekhar
authors: J Venkateshwar
authors: JV
authors: Rao
authors: Smita C
authors: SC
authors: Pawar
mesh_terms: D017304:Annexin A5 / Q000737:chemistry; D001665:Binding Sites; D020134:Catalytic Domain; D003198:Computer Simulation; D005109:Extracellular Matrix / Q000737:chemistry; D051417:Focal Adhesion Kinase 1 / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D039641:Integrin beta Chains / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D008958:Models, Molecular; D062105:Molecular Docking Simulation; D009362:Neoplasm Metastasis; D009369:Neoplasms / Q000737:chemistry / Q000378:metabolism*; D009389:Neovascularization, Pathologic / Q000378:metabolism; D011485:Protein Binding; D054462:p21-Activated Kinases / Q000737:chemistry / Q000378:metabolism
chemical_list: D017304:Annexin A5; D039641:Integrin beta Chains; C084344:integrin beta5; C116367:PAK4 protein, human; D051417:Focal Adhesion Kinase 1; D054462:p21-Activated Kinases