title: Physiological and proteomics analyses of Holm oak (Quercus ilex subsp. ballota [Desf.] Samp.) responses to Phytophthora cinnamomi.
abstract: Phytophthora cinnamomi is one of the agents that trigger the decline syndrome in Quercus spp., this being a serious threat to Mediterranean Holm oak forest sustainability and reforestation programs. Quercus ilex responses to Phytophthora cinnamomi have been studied in one-year olds seedlings from two Andalucía provenances, assessing the physiological water status and photosynthesis-related parameters. Upon inoculation with mycelium a reduction in water content, chlorophyll fluorescence, stomatal conductance and gas exchange was observed along a 90 days post inoculation period in both provenances. The reduction was higher in the most susceptible (SSA) provenance, than in the most tolerant (PCO), being these typical plant responses to drought stress. Leaf protein profiles were analyzed in non-inoculated and inoculated seedlings from the two provenances by using a 2-DE coupled to MS proteomics strategy. Ninety seven proteins changing in abundance in response to the inoculation were successfully identified after MALDI-TOF-TOF analyses. The largest group of variable identified proteins were chloroplasts ones, and they were involved in the photosynthesis, Calvin cycle and carbohydrate metabolism. It was noted that a general tendency was a decrease in the protein abundance as a consequence of the inoculation, being it less accused in the least susceptible, the Northern provenance (PCO), than in the most susceptible, the Southern provenance (SSA). This trend is clearly manifested in photosynthesis, amino acid metabolism and stress/defence proteins. On the contrary, some proteins related to starch biosynthesis, glycolysis and stress related peroxiredoxin showed an increase upon inoculation. These changes in protein abundance were correlated to the estimated physiological parameters and have been frequently observed in plants subjected to drought stress.
authors: Agricultural and Plant Biochemistry and Proteomics Research Group, Department of Biochemistry and Molecular Biology, University of Córdoba, Agrifood Campus of International Excellence, ceiA3, 14071 Córdoba, Spain; Laboratoire des Plantes Extrêmophiles (LPE), Centre de Biotechnologie à la Technopole de Borj-Cedria (CBBC), BP 901, Hammam-Lif 2050, Tunisia. Electronic address: sghaierbesma@yahoo.fr.
authors: Besma
authors: B
authors: Sghaier-Hammami
authors: José
authors: J
authors: Valero-Galvàn
authors: M Cristina
authors: MC
authors: Romero-Rodríguez
authors: Rafael Ma
authors: RM
authors: Navarro-Cerrillo
authors: Chedly
authors: C
authors: Abdelly
authors: Jesús
authors: J
authors: Jorrín-Novo
mesh_terms: D018506:Gene Expression Regulation, Plant / Q000235:genetics / Q000502:physiology; D010838:Phytophthora / Q000502:physiology*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D040901:Proteomics / Q000379:methods; D029963:Quercus / Q000382:microbiology*
chemical_list: D010940:Plant Proteins
keywords: Holm oak decline; Holm oak proteomics; Phytophthora cinnamomi; Quercus ilex