title: αV-integrins are required for mechanotransduction in MDCK epithelial cells.
abstract: The properties of epithelial cells within tissues are regulated by their immediate microenvironment, which consists of neighboring cells and the extracellular matrix (ECM). Integrin heterodimers orchestrate dynamic assembly and disassembly of cell-ECM connections and thereby convey biochemical and mechanical information from the ECM into cells. However, the specific contributions and functional hierarchy between different integrin heterodimers in the regulation of focal adhesion dynamics in epithelial cells are incompletely understood. Here, we have studied the functions of RGD-binding αV-integrins in a Madin Darby Canine Kidney (MDCK) cell model and found that αV-integrins regulate the maturation of focal adhesions (FAs) and cell spreading. αV-integrin-deficient MDCK cells bound collagen I (Col I) substrate via α2β1-integrins but failed to efficiently recruit FA components such as talin, focal adhesion kinase (FAK), vinculin and integrin-linked kinase (ILK). The apparent inability to mature α2β1-integrin-mediated FAs and link them to cellular actin cytoskeleton led to disrupted mechanotransduction in αV-integrin deficient cells seeded onto Col I substrate.
authors: Biocenter Oulu, Oulu Center for Cell-Matrix Research, Department of Medical Biochemistry and Molecular Biology, University of Oulu, Oulu, Finland.
authors: Terhi P
authors: TP
authors: Teräväinen
authors: Satu M
authors: SM
authors: Myllymäki
authors: Jens
authors: J
authors: Friedrichs
authors: Nico
authors: N
authors: Strohmeyer
authors: Jose V
authors: JV
authors: Moyano
authors: Chuanyue
authors: C
authors: Wu
authors: Karl S
authors: KS
authors: Matlin
authors: Daniel J
authors: DJ
authors: Muller
authors: Aki
authors: A
authors: Manninen
mesh_terms: D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D024042:Collagen Type I / Q000494:pharmacology; D004285:Dogs; D004789:Enzyme Activation / Q000187:drug effects; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism*; D005109:Extracellular Matrix / Q000187:drug effects / Q000378:metabolism; D051416:Focal Adhesion Protein-Tyrosine Kinases / Q000378:metabolism; D022001:Focal Adhesions / Q000187:drug effects / Q000378:metabolism; D055785:Gene Knockdown Techniques; D039564:Integrin alphaV / Q000378:metabolism*; D019012:Integrin beta1 / Q000378:metabolism; D007797:Laminin / Q000378:metabolism; D061985:Madin Darby Canine Kidney Cells; D040542:Mechanotransduction, Cellular / Q000187:drug effects; D051379:Mice; D009842:Oligopeptides / Q000378:metabolism; D011485:Protein Binding / Q000187:drug effects
chemical_list: D024042:Collagen Type I; D039564:Integrin alphaV; D019012:Integrin beta1; D007797:Laminin; D009842:Oligopeptides; C047981:arginyl-glycyl-aspartic acid; D051416:Focal Adhesion Protein-Tyrosine Kinases
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