title: Application of surfactant assisted dispersive liquid-liquid microextraction as an efficient sample treatment technique for preconcentration and trace detection of zonisamide and carbamazepine in urine and plasma samples.
abstract: A simple, rapid, and efficient method, based on surfactant assisted dispersive liquid-liquid microextraction (SA-DLLME), followed by high performance liquid chromatography (HPLC) has been developed for simultaneous preconcentration and trace detection of zonisamide and carbamazepine in biological samples. A conventional cationic surfactant called cethyltrimethyl ammonium bromide (CTAB) was used as a disperser agent in the proposed approach. 1.5 mL of CTAB (0.45 mmol L(-1)) (disperser solvent) containing 50.0 μL of 1-octanol (extraction solvent) was injected rapidly into the 7.0 mL of water or diluted plasma or urine. A cloudy solution (water, 1-octanol, and CTAB) was formed in the test tube. After formation of cloudy solution, the mixture was centrifuged and 20 μL of collected phase was injected into HPLC for subsequent analysis. Some parameters such as the type and volume of the extraction solvent, the type and concentration of surfactant, pH, ionic strength and centrifugation time were evaluated and optimized. Under optimum extraction conditions, the limits of detections (LODs) were 2.1 and 1.5 μg L(-1) (based on 3Sb/m) for urine samples, and 2.3 and 1.6 μg L(-1) for plasma samples. Linear dynamic range of 5-300 and 5-200 μg L(-1) were obtained for zonisamide and carbamazepine in all samples. Finally, the applicability of the proposed method was evaluated by extraction and determination of the drugs in urine and plasma samples.
authors: Department of Chemistry, Shahid Beheshti University, G.C., Tehran 1983963113, Iran.
authors: Mohammad
authors: M
authors: Behbahani
authors: Fatemeh
authors: F
authors: Najafi
authors: Saman
authors: S
authors: Bagheri
authors: Majid Kalate
authors: MK
authors: Bojdi
authors: Mani
authors: M
authors: Salarian
authors: Akbar
authors: A
authors: Bagheri
mesh_terms: D020003:1-Octanol / Q000737:chemistry; D002220:Carbamazepine / Q000097:blood / Q000302:isolation & purification* / Q000652:urine; D000077286:Cetrimonium; D002593:Cetrimonium Compounds / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D020920:Dyssomnias; D006801:Humans; D007555:Isoxazoles / Q000097:blood / Q000302:isolation & purification* / Q000652:urine; D016014:Linear Models; D059627:Liquid Phase Microextraction / Q000379:methods*; D009994:Osmolar Concentration; D015203:Reproducibility of Results; D012972:Sodium Hydroxide / Q000737:chemistry; D013501:Surface-Active Agents / Q000737:chemistry*; D000078305:Zonisamide
chemical_list: D002593:Cetrimonium Compounds; D007555:Isoxazoles; D013501:Surface-Active Agents; D002220:Carbamazepine; D000078305:Zonisamide; D012972:Sodium Hydroxide; D020003:1-Octanol; D000077286:Cetrimonium
keywords: Biological samples; Carbamazepine; Sample treatment; Surfactant assisted dispersive liquid–liquid microextraction; Zonisamide