title: Conditional targeted genome editing using somatically expressed TALENs in C. elegans.
abstract: We have developed a method for the generation of conditional knockouts in Caenorhabditis elegans by expressing transcription activator-like effector nucleases (TALENs) in somatic cells. Using germline transformation with plasmids encoding TALENs under the control of an inducible or tissue-specific promoter, we observed effective gene modifications and resulting phenotypes in specific developmental stages and tissues. We further used this method to bypass the embryonic requirement of cor-1, which encodes the homolog of human severe combined immunodeficiency (SCID) protein coronin, and we determined its essential role in cell migration in larval Q-cell lineages. Our results show that TALENs expressed in the somatic cells of model organisms provide a versatile tool for functional genomics.
authors: 1] National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Chaoyang District, Beijing, China. [2] School of Life Sciences, Peking University, Beijing, China. [3].
authors: Ze
authors: Z
authors: Cheng
authors: Peishan
authors: P
authors: Yi
authors: Xiangming
authors: X
authors: Wang
authors: Yongping
authors: Y
authors: Chai
authors: Guoxin
authors: G
authors: Feng
authors: Yihong
authors: Y
authors: Yang
authors: Xing
authors: X
authors: Liang
authors: Zhiwen
authors: Z
authors: Zhu
authors: Wei
authors: W
authors: Li
authors: Guangshuo
authors: G
authors: Ou
mesh_terms: D000818:Animals; D001483:Base Sequence; D017173:Caenorhabditis elegans / Q000166:cytology / Q000235:genetics*; D019070:Cell Lineage; D002465:Cell Movement; D003851:Deoxyribonucleases / Q000378:metabolism*; D049751:Genome, Helminth / Q000235:genetics*; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006801:Humans; D008164:Luminescent Proteins / Q000378:metabolism; D040542:Mechanotransduction, Cellular; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D009474:Neurons / Q000166:cytology / Q000378:metabolism; D017393:RNA Editing / Q000235:genetics*; D015534:Trans-Activators / Q000378:metabolism*; D000097573:Red Fluorescent Protein
chemical_list: D003851:Deoxyribonucleases; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins; D015534:Trans-Activators; D000097573:Red Fluorescent Protein
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