title: Genome-wide analysis of Nilaparvata lugens nymphal responses to high-density and low-quality rice hosts.
abstract: The brown planthopper (BPH) Nilaparvata lugens is an economically important pest on rice plants. In this study, the higher population density and yellow-ripe stage of rice plants were used to construct adverse survival conditions (ASC) against BPH nymphs. Simultaneously, the low population density and tillering stage of rice plants were used to establish a suitable survival condition (SSC) as a control. Solexa/Illumina sequencing was used to identify genes of BPH nymphs responding to ASC. Significantly longer duration development of BPH nymphs and significantly lower brachypterous ratio of BPH adults were observed by ASC compared with SSC. A total of 2 544 differentially expressed genes (DEGs) were obtained and analyzed by BLASTx, Gene Ontology and KEGG Orthology. Gene ontology analysis revealed that the DEGs were mainly involved in categories of cell, cell part, cellular process, binding, catalytic, organelle and metabolic processes. 1 138 DEGs having enzyme commission numbers were assigned to different metabolic pathways. The largest clusters were neurodegenerative diseases (137, 12.0%), followed by carbohydrate metabolism (113, 9.9%), amino acid metabolism (94, 8.3%), nucleotide metabolism (76, 6.7%), energy metabolism (64, 5.6%), translation (60, 5.3%), lipid metabolism (58, 5.1%), and folding, sorting and degradation (52, 4.6%). Expressing profile of 11 DEGs during eight nymphal developmental stages of BPH were analyzed by quantitative real-time polymerase chain reaction. The 11 genes exhibited differential expression between ASC and SSC during at least one developmental stage. The DEGs identified in this study provide molecular proof of how BPH reconfigures its gene expression profile to adapt to overcrowding and low-quality hosts.
authors: Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070.
authors: Ding-Bang
authors: DB
authors: Hu
authors: Bing-Qing
authors: BQ
authors: Luo
authors: Jie
authors: J
authors: Li
authors: Yu
authors: Y
authors: Han
authors: Ting-Ru
authors: TR
authors: Jiang
authors: Jia
authors: J
authors: Liu
authors: Gang
authors: G
authors: Wu
authors: Hong-Xia
authors: HX
authors: Hua
authors: Yan-Fei
authors: YF
authors: Xiong
authors: Jun-Sheng
authors: JS
authors: Li
mesh_terms: D000818:Animals; D005260:Female; D020869:Gene Expression Profiling; D015723:Gene Library; D049750:Genome, Insect; D006430:Hemiptera / Q000235:genetics* / Q000378:metabolism; D060434:Herbivory / Q000235:genetics*; D008297:Male; D058977:Molecular Sequence Annotation; D009758:Nymph / Q000378:metabolism; D012275:Oryza / Q000502:physiology*; D060888:Real-Time Polymerase Chain Reaction; D013312:Stress, Physiological; D014921:Wings, Animal / Q000254:growth & development
keywords: Nilaparvata lugens; Solexa sequencing; gene expression; low-quality host; metabolic pathway; overcrowding