title: Proteomic analysis of extremely severe hand, foot and mouth disease infected by enterovirus 71.
abstract: BACKGROUND
  To clarify the molecular mechanisms that participate in the severe hand, foot and mouth disease (HFMD) infected by Enterovirus 71 and to detect any related protein biomarkers, we performed proteomic analysis of protein extracts from 5 extremely severe HFMD children and 5 healthy children.
  
  
  METHODS
  The protein profiles of them were compared using two-dimensional electrophoresis. Differentially expressed proteins were identified using mass spectrometry. Functional classifications of these proteins were based on the PANTHER. The interaction network of the differentially expressed protein was generated with Pathway Studio.
  
  
  RESULTS
  A total of 38 differentially expressed proteins were identified. Functional classifications of these proteins indicated a series of altered cellular processes as a consequence of the severe HFMD. These results provided not only new insights into the pathogenesis of severe HFMD, but also implications of potential therapeutic designs.
  
  
  CONCLUSIONS
  Our results suggested the possible pathways that could be the potential targets for novel therapy: viral protection, complement system and peroxide elimination.
authors: Guangzhou Women and Children′s Medical Center, Guangzhou 510120, Guangdong, China.
authors: Li
authors: L
authors: Deng
authors: Hong-Ling
authors: HL
authors: Jia
authors: Chao-Wu
authors: CW
authors: Liu
authors: Yu-Fen
authors: YF
authors: Xu
authors: Li-Jia
authors: LJ
authors: Mao
authors: Chun-Hui
authors: CH
authors: He
authors: Gen-Quan
authors: GQ
authors: Yin
authors: Jun-Hong
authors: JH
authors: Lin
authors: Jian-Ping
authors: JP
authors: Tao
authors: Li
authors: L
authors: Zhu
mesh_terms: D001798:Blood Proteins / Q000032:analysis; D015153:Blotting, Western; D015180:Electrophoresis, Gel, Two-Dimensional; D029821:Enterovirus A, Human / Q000302:isolation & purification*; D006232:Hand, Foot and Mouth Disease / Q000097:blood*; D006801:Humans; D060066:Protein Interaction Maps; D020543:Proteome / Q000032:analysis*; D040901:Proteomics; D015203:Reproducibility of Results; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D001798:Blood Proteins; D020543:Proteome
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