title: 50 Hz electromagnetic field produced changes in FTIR spectroscopy associated with mitochondrial transmembrane potential reduction in neuronal-like SH-SY5Y cells.
abstract: SH-SY5Y neuroblastoma cells were used as an experimental model to study the effects of 50 Hz electromagnetic field, in the range from 50 µ T to 1.4 mT. Fourier transform infrared spectroscopy analysis evidenced a reduction in intensity of the amide A band and a slight increase of vibration bands at 2921 cm(-1) and 2853 cm(-1) corresponding to methylene groups. A further increase of the magnetic field intensity of exposure up to 0.8 mT and 1.4 mT produced a clear increase in intensity of CH2 vibration bands. Moreover, it has been observed some alterations in the amide I region, such as a shifted peak of the amide I band to a smaller wavenumber, probably due to protein conformational changes. These results suggested that exposure to extremely low electromagnetic fields influenced lipid components of cellular membrane and the N-H in-plane bending and C-N stretching vibrations of peptide linkages, modifying the secondary structures of α -helix and β -sheet contents and producing unfolding process in cell membrane proteins. The observed changes after exposure to 50 Hz electromagnetic field higher than 0.8 mT were associated with a significant reduction of cell viability and reduced mitochondrial transmembrane potential.
authors: Department of Physics, University of Messina, 98165 Messina, Italy.
authors: Emanuele
authors: E
authors: Calabrò
authors: Salvatore
authors: S
authors: Condello
authors: Monica
authors: M
authors: Currò
authors: Nadia
authors: N
authors: Ferlazzo
authors: Mercurio
authors: M
authors: Vecchio
authors: Daniela
authors: D
authors: Caccamo
authors: Salvatore
authors: S
authors: Magazù
authors: Riccardo
authors: R
authors: Ientile
mesh_terms: D045744:Cell Line, Tumor; D002470:Cell Survival / Q000502:physiology; D004574:Electromagnetic Fields; D006801:Humans; D053078:Membrane Potential, Mitochondrial / Q000502:physiology*; D013055:Spectrophotometry, Infrared; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*
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