title: Effects of cyclosporine a in ex vivo reperfused pig lungs.
abstract: OBJECTIVE
  Several works highlight the role of CsA in the prevention of IRI, but none focus on isolated lungs. Our objective was to evaluate the effects of CsA on IRI on ex vivo reperfused pig lungs.
  
  
  METHODS
  Thirty-two pairs of pig lungs were collected and stored for 30 minutes at 4 °C. The study was performed in four groups. First, a control group and then three groups receiving different concentrations of CsA (1, 10, and 30 μM) at two different times: once at the moment of lung procurement and another during the reperfusion procedure. The ex vivo lung preparation was set up using an extracorporeal perfusion circuit. Gas exchange parameters, pulmonary hemodynamics, and biological markers of lung injury were collected for the evaluation.
  
  
  RESULTS
  CsA improved the PaO2 /FiO2 ratio, but it also increased PAP, Pcap, and pulmonary vascular resistances with dose-dependent effects. Lungs treated with high doses of CsA displayed higher capillary-alveolar permeability to proteins, lower AFC capacities, and elevated concentrations of pro-inflammatory cytokines.
  
  
  CONCLUSIONS
  These data suggest a possible deleterious imbalance between the beneficial cell properties of CsA in IRI and its hemodynamic effects on microvascularization.
authors: Emergency Department, Grenoble University Hospital, Grenoble Cedex, France; TIMC IMAG Laboratory, UMR 5525, La Tronche Cedex, France.
authors: Stéphane
authors: S
authors: Gennai
authors: Redha
authors: R
authors: Souilamas
authors: Maxime
authors: M
authors: Maignan
authors: Angélique
authors: A
authors: Brouta
authors: Christophe
authors: C
authors: Pison
authors: Eric
authors: E
authors: Fontaine
authors: Raphaël
authors: R
authors: Briot
mesh_terms: D000818:Animals; D002199:Capillary Permeability / Q000187:drug effects*; D016572:Cyclosporine / Q000494:pharmacology*; D016207:Cytokines / Q000378:metabolism; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D055370:Lung Injury / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D010477:Perfusion; D011650:Pulmonary Alveoli / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D011659:Pulmonary Gas Exchange / Q000187:drug effects*; D013552:Swine
chemical_list: D016207:Cytokines; D007166:Immunosuppressive Agents; D016572:Cyclosporine
keywords: ischemia/reperfusion injury; lung physiology; lung transplantation; pulmonary function