title: Preparation and application of hollow molecularly imprinted polymers with a super-high selectivity to the template protein.
abstract: Protein-imprinted polymers with hollow cores that have a super-high imprinting factor were prepared by etching the core of the surface-imprinted polymers that used silica particles as the support. Lysozyme as template was modified onto the surface of silica particles by a covalent method, and after polymerization and the removal of template molecules, channels through the polymer layer were formed, which allowed a single-protein molecule to come into the hollow core and attach to the binding sites inside the polymer layer. The adsorption experiments demonstrated that the hollow imprinted polymers had an extremely high binding capacity and selectivity, and thus a super-high imprinting factor was obtained. The as-prepared imprinted polymers were used to separate the template lysozyme from egg white successfully, indicating its high selectivity and potential application in the field of separation of protein from real samples.
authors: State Key Laboratory of Medicinal Chemical Biology, Department of Chemistry, Nankai University, Tianjin, China.
authors: Yang
authors: Y
authors: Chen
authors: Xi-Wen
authors: XW
authors: He
authors: Jie
authors: J
authors: Mao
authors: Wen-You
authors: WY
authors: Li
authors: Yu-Kui
authors: YK
authors: Zhang
mesh_terms: D000327:Adsorption; D000818:Animals; D002645:Chickens; D004527:Egg Proteins / Q000737:chemistry / Q000302:isolation & purification*; D004531:Eggs / Q000032:analysis*; D054802:Molecular Imprinting; D009113:Muramidase / Q000737:chemistry / Q000302:isolation & purification*; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry*; D052616:Solid Phase Extraction / Q000295:instrumentation / Q000379:methods*
chemical_list: D004527:Egg Proteins; D011108:Polymers; D009113:Muramidase
keywords: Etching; Hollow core; Lysozyme; Molecularly imprinted polymers; Protein separation