title: HPTLC-densitometric method for simultaneous determination of salmeterol xinafoate and fluticasone propionate in dry powder inhalers.
abstract: A high performance thin layer chromatography (HPTLC) method was developed and validated for determination of two anti-asthmatic drugs, salmeterol xinafoate and fluticasone propionate in co-formulations. Study was performed on pre-coated silica gel HPTLC plates using n-hexane:ethyl acetate:acetic acid (5:10:0.2) as a mobile phase. A TLC scanner set at 250 nm was used for direct evaluation of the chromatograms in reflectance/absorbance mode. Method was validated according to ICH guidelines. Determination coefficients of calibration curves were found 0.9977 and 0.9936 in the ranges 100-1000 and 200-2000 ng band(-1) for salmeterol and fluticasone, respectively. Method had an accuracy of 99.5% for salmeterol and 102.01% for fluticasone. Method had the potential to determine these drugs simultaneously from dosage forms without any interference.
authors: Department of Pharmaceutical Chemistry, School of Pharmacy, Addis Ababa University, Ethiopia.
authors: Lantider
authors: L
authors: Kasaye
authors: Ariaya
authors: A
authors: Hymete
authors: Abdel-Maaboud I
authors: AM
authors: Mohamed
keywords: Densitometric detection; High performance thin layer chromatography; Pharmaceuticals; Salmeterol xinafoate and fluticasone propionate combinations
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