title: The preferential binding of a sensory organ specific odorant binding protein of the alfalfa plant bug Adelphocoris lineolatus AlinOBP10 to biologically active host plant volatiles.
abstract: Semiochemicals such as sex pheromones and plant volatiles are crucial components of insect mating systems and host plant localization. In the olfactory signal transduction pathway, odorant-binding proteins (OBPs) are important elements that function in the first step of the pathway by carrying hydrophobic semiochemicals across the sensillum lymph to the olfactory receptors (ORs). In this study, we examined the binding affinities of semiochemicals to AlinOBP10, a putative OBP from the alfalfa plant bug, Adelphocoris lineolatus, that we demonstrate is expressed mainly in sensory organs. We then characterized the biological activities of the high affinity semiochemicals by measuring their electrophysiological activities in antennae and behavioral responses in the plant bug. AlinOBP10 displayed weak binding affinities to two major putative pheromone components, hexyl butyrate and (E)-2-hexenyl butyrate. In contrast, AlinOBP10 exhibited higher binding affinities to six host plant volatiles, namely myrcene, β-pinene, β-ionone, 3-hexanone, (E)-2-hexenal, and 1-hexanol. The biological activities of these six putative ligands were further studied in electroantennogram recordings and Y-tube olfactometer trials. The three compounds, (E)-2-hexenal, 1-hexanol, and 3-hexanone elicited strong electrophysiological responses, but elicited distinct behaviors. While 3-hexanone was attractive to female adults, (E)-2-hexenal and 1-hexanol were significant repellents. Although a weak electrophysiological response was elicited with β-pinene, it was a strong repellent. These results demonstrate that AlinOBP10 can interact with attractants, as well as repellents, with some specificity toward plant volatiles over sex pheromones.
authors: Key Laboratory of Integrated Management of Crop Diseases and Pests (Ministry of Education), College of Plant Protection, Nanjing Agricultural University, Nanjing, 210095, China.
authors: Liang
authors: L
authors: Sun
authors: Shao-Hua
authors: SH
authors: Gu
authors: Hai-Jun
authors: HJ
authors: Xiao
authors: Jing-Jiang
authors: JJ
authors: Zhou
authors: Yu-Yuan
authors: YY
authors: Guo
authors: Ze-Wen
authors: ZW
authors: Liu
authors: Yong-Jun
authors: YJ
authors: Zhang
mesh_terms: D000818:Animals; D057506:Arthropod Antennae / Q000378:metabolism; D001522:Behavior, Animal / Q000502:physiology; D005260:Female; D020062:Heteroptera / Q000502:physiology*; D008297:Male; D010675:Pheromones / Q000378:metabolism*; D018035:Receptors, Odorant / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D055549:Volatile Organic Compounds / Q000378:metabolism
chemical_list: D010675:Pheromones; D018035:Receptors, Odorant; D011994:Recombinant Proteins; D055549:Volatile Organic Compounds; C045648:odorant-binding protein
references: PLoS Biol. 2007 May;5(5):e118
references: 17456006
references: Proc Natl Acad Sci U S A. 1985 Dec;82(24):8827-31
references: 3001718
references: Protein Sci. 1993 Mar;2(3):420-8
references: 8453379
references: Insect Biochem Mol Biol. 2011 Apr;41(4):254-63
references: 21232599
references: J Chem Ecol. 2012 Sep;38(9):1163-70
references: 23053914
references: Insect Biochem Mol Biol. 2009 May-Jun;39(5-6):414-9
references: 19328854
references: J Chem Ecol. 2010 Mar;36(3):245-8
references: 20191395
references: J Chem Ecol. 1994 Dec;20(12):3039-50
references: 24241975
references: J Chem Ecol. 2012 May;38(5):512-24
references: 22544334
references: Annu Rev Entomol. 2005;50:31-52
references: 15355239
references: Science. 2010 Aug 27;329(5995):1075-8
references: 20798319
references: Nature. 1981 Sep 10-16;293(5828):161-3
references: 18074618
references: PLoS One. 2010 Mar 01;5(3):e9471
references: 20208991
references: Chem Biol. 2000 Feb;7(2):143-51
references: 10662696
references: J Chem Ecol. 1995 Aug;21(8):1217-27
references: 24234527
references: Insect Biochem Mol Biol. 1993 Jun;23(4):449-56
references: 8508187
references: Ying Yong Sheng Tai Xue Bao. 2007 Apr;18(4):859-64
references: 17615885
references: J Chem Ecol. 2003 Apr;29(4):931-44
references: 12775153
references: Vitam Horm. 2010;83:241-72
references: 20831949
references: J Chem Ecol. 2010 May;36(5):467-78
references: 20401755
references: Trends Plant Sci. 2005 Jun;10(6):269-74
references: 15949760
references: J Exp Biol. 2003 Feb;206(Pt 4):715-24
references: 12517989
references: Genes Brain Behav. 2011 Aug;10(6):648-57
references: 21605338
references: J Chem Ecol. 2005 Mar;31(3):481-95
references: 15898496
references: FEBS Lett. 2004 Jan 30;558(1-3):23-6
references: 14759510
references: J Chem Ecol. 2004 May;30(5):889-911
references: 15274438
references: Biochem Biophys Res Commun. 1999 Dec 20;266(2):386-91
references: 10600513
references: Insect Mol Biol. 2010 Mar;19 Suppl 2:113-22
references: 20482644
references: FEBS Lett. 1999 Dec 24;464(1-2):85-90
references: 10611489
references: Neuron. 2005 Jan 20;45(2):193-200
references: 15664171
references: Insect Biochem Mol Biol. 2009 Jul;39(7):448-56
references: 19364529
references: Insect Mol Biol. 2006 Jun;15(3):383-91
references: 16756557
references: Bull Entomol Res. 2009 Dec;99(6):543-50
references: 19222869
references: PLoS One. 2011;6(12):e28921
references: 22174925
references: Environ Entomol. 2008 Dec;37(6):1410-5
references: 19161683
references: Science. 2010 May 28;328(5982):1151-4
references: 20466880
references: Chem Senses. 2009 Jul;34(6):503-12
references: 19497961
references: Methods. 2001 Dec;25(4):402-8
references: 11846609
references: PLoS One. 2012;7(3):e32759
references: 22427877
references: Cell Mol Life Sci. 2006 Jul;63(14):1658-76
references: 16786224
references: J Chem Ecol. 2010 Dec;36(12):1293-305
references: 20981477
references: Cell. 2008 Jun 27;133(7):1255-1265
references: 18585358
references: Proc Natl Acad Sci U S A. 1994 Dec 6;91(25):11836-40
references: 11607499
references: Chem Senses. 2006 Jul;31(6):547-55
references: 16679489
references: J Chem Ecol. 1994 Dec;20(12):3251-67
references: 24241990
references: Invert Neurosci. 2002 Oct;4(4):165-74
references: 12488967
references: Arch Insect Biochem Physiol. 2011 Jun;77(2):81-99
references: 21541988
references: J Chem Ecol. 2004 Aug;30(8):1547-64
references: 15537158