title: Extracts of Salvia miltiorrhiza Bunge on the cytokines of rat endometriosis models.
abstract: Endometriosis is a common mysterious and fascinating gynaecological condition with diverse clinical manifestations, highly variable and unpredictable clinical course with decreased quality of life. Clinically, Salvia miltiorrhiza Bunge (SMB, Chinese Danshen) has been applied to treat endometriosis and get satisfactory results. The present study was aimed to explore the effects of the extracts of SMB (ESMB) on the serum levels of cancer antigen 125 (CA-125) and the levels of interleukin (IL)-13, IL-18 and tumor necrosis factor-alpha (TNF-alpha) in the peritoneal fluids of rat endometriosis models. Three extraction methods for SMB were compared, which are the sample extracted with conventional method, the sample extracted with espresso coffee machine and the commercial condensed powder of natural products. We determined tanshinone IIA, salvianolic acid B and danshensu in the ESMB of different extraction methods. Forty female Sprague-Dawley (SD) rats were randomly divided into ESMB group, Danazol (positive control) group, model group and the sham-operation group (Sham group). After all the treatment ended, the serum levels of CA125 and the levels of IL-13, IL-18 and TNF-alpha in the peritoneal fluids of rat endometriosis models were measured using enzyme-linked immune-sorbent assay (ELISA) as directed by the manufacturer. The extraction efficiency of the ESMB samples extracted with coffee machine ranged from 600µm to 710µm was the highest. The serum levels of CA-125 and the levels of IL-18 and TNF-alpha in the peritoneal fluids of ESMB group, Danazol group and Sham group were significantly lower than those of the Model group (P<0.05). The serum levels of CA-125 and the levels of IL-18 and TNF-alpha in the peritoneal fluids of Danazol group and ESMB group were significantly higher than those of Sham group, respectively (P<0.05), and no marked difference existed between them (P>0.05). The levels of IL-13 in the peritoneal fluids of ESMB group, Danazol group and Sham group were significantly higher than those of the Model group (P<0.05). The levels of IL-13 in the peritoneal fluids of ESMB group and Danazol group were significantly lower than those of Sham group (P<0.05), and there was no marked difference between ESMB group and Sham group (P>0.05). ESMB shows promises in treating endometriosis by markedly decreasing the serum levels of CA-125 and the levels of IL-18 and TNF-alpha in the peritoneal fluids and significantly increasing the levels of IL-13 in the peritoneal fluids.
authors: School of Medicine, Lishui University, Lishui, 323000, Zhejiang, China.
authors: Zan-Hua
authors: ZH
authors: Zhou
authors: Qing
authors: Q
authors: Weng
authors: Jian-Hong
authors: JH
authors: Zhou
authors: Jue
authors: J
authors: Zhou
mesh_terms: D000818:Animals; D001202:Ascitic Fluid / Q000378:metabolism*; D018394:CA-125 Antigen / Q000097:blood / Q000378:metabolism*; D016207:Cytokines / Q000097:blood / Q000378:metabolism*; D003613:Danazol / Q000494:pharmacology / Q000627:therapeutic use; D004195:Disease Models, Animal; D004365:Drugs, Chinese Herbal / Q000494:pharmacology / Q000627:therapeutic use*; D004715:Endometriosis / Q000188:drug therapy*; D004965:Estrogen Antagonists / Q000494:pharmacology / Q000627:therapeutic use; D005260:Female; D018793:Interleukin-13 / Q000097:blood / Q000378:metabolism; D020382:Interleukin-18 / Q000097:blood / Q000378:metabolism; D010529:Peritoneal Cavity / Q000473:pathology; D008517:Phytotherapy; D017207:Rats, Sprague-Dawley; D027561:Salvia miltiorrhiza; D014409:Tumor Necrosis Factor-alpha / Q000097:blood / Q000378:metabolism
chemical_list: D018394:CA-125 Antigen; D016207:Cytokines; D004365:Drugs, Chinese Herbal; D004965:Estrogen Antagonists; D018793:Interleukin-13; D020382:Interleukin-18; D014409:Tumor Necrosis Factor-alpha; C028972:dan-shen root extract; D003613:Danazol
keywords: Endometriosis; Salvia miltiorrhiza Bunge (SMB); Tanshinone IIA
references: Eur J Drug Metab Pharmacokinet. 2008 Jan-Mar;33(1):9-16
references: 18543579
references: Hum Reprod Update. 2011 Sep-Oct;17(5):637-53
references: 21672902
references: Hum Reprod Update. 2007 Sep-Oct;13(5):487-99
references: 17575287
references: Fertil Steril. 2011 Sep;96(3):697-9
references: 21762896
references: Basic Clin Pharmacol Toxicol. 2010 Jan;106(1):30-7
references: 19906051
references: Fertil Steril. 2011 Jan;95(1):393-6
references: 20934691
references: Niger J Med. 2011 Apr-Jun;20(2):191-9
references: 21970227
references: Cochrane Database Syst Rev. 2007 Oct 17;(4):CD000068
references: 17943735
references: Cochrane Database Syst Rev. 2009 Jul 08;(3):CD006568
references: 19588398
references: Evid Based Complement Alternat Med. 2011;2011:378907
references: 21151668
references: Hum Reprod Update. 2010 Nov-Dec;16(6):651-74
references: 20462942
references: Proteomics. 2010 Mar;10(5):914-29
references: 20049856
references: Hum Reprod. 2002 Feb;17(2):426-31
references: 11821289
references: Food Chem Toxicol. 2008 Jan;46(1):73-81
references: 17719161
references: J Clin Pharmacol. 2005 Dec;45(12):1345-59
references: 16291709
references: Phytomedicine. 2010 Mar;17(3-4):212-8
references: 19800776
references: J Ethnopharmacol. 2011 Mar 8;134(1):147-55
references: 21134432
references: Xenobiotica. 2007 Jun;37(6):635-78
references: 17614009
references: Neurosci Lett. 2010 Feb 12;470(2):126-9
references: 20045721
references: Fertil Steril. 1997 May;67(5):865-9
references: 9130891
references: Med Sci Monit. 2011 Apr;17(4):RA92-9
references: 21455119
references: Lancet. 2004 Nov 13-19;364(9447):1789-99
references: 15541453
references: Bioresour Technol. 2008 Nov;99(16):7892-6
references: 18396038
references: Gynecol Obstet Invest. 2002;53 Suppl 1:52-62
references: 11834869
references: Fertil Steril. 2004 Oct;82(4):878-84
references: 15482763
references: Fertil Steril. 2003 Oct;80(4):889-94
references: 14556808
references: Biol Pharm Bull. 2010;33(11):1828-34
references: 21048307
references: Am J Obstet Gynecol. 1994 Sep;171(3):740-5
references: 8092224
references: Anal Chim Acta. 2007 Apr 25;589(2):231-8
references: 17418186
references: J Altern Complement Med. 2007 Nov;13(9):969-76
references: 18047443
references: Neuropharmacology. 2010 Dec;59(7-8):595-604
references: 20800073
references: Duodecim. 2011;127(17):1827-35
references: 21995119
references: J Cell Biochem. 2008 May 1;104(1):15-26
references: 17979138