title: Regulation of cell wall synthesis by the clathrin light chain is essential for viability in Schizosaccharomyces pombe.
abstract: The regulation of cell wall synthesis by the clathrin light chain has been addressed. Schizosaccharomyces pombe clc1Δ mutant was inviable in the absence of osmotic stabilization; when grown in sorbitol-supplemented medium clc1Δ cells grew slowly, formed aggregates, and had strong defects in morphology. Additionally, clc1Δ cells exhibited an altered cell wall composition. A mutant that allowed modulating the amount of Clc1p was created to analyze in more detail the dependence of cell wall synthesis on clathrin. A 40% reduction in the amount of Clc1p did not affect acid phosphatase secretion and bulk lipid internalization. Under these conditions, β(1,3)glucan synthase activity and cell wall synthesis were reduced. Also, the delivery of glucan synthases to the cell surface, and the secretion of the Eng1p glucanase were defective. These results suggest that the defects in the cell wall observed in the conditional mutant were due to a defective secretion of enzymes involved in the synthesis/remodelling of this structure, rather than to their endocytosis. Our results show that a reduction in the amount of clathrin that has minor effects on general vesicle trafficking has a strong impact on cell wall synthesis, and suggest that this is the reason for the lethality of clc1Δ cells in the absence of osmotic stabilization.
authors: Departamento de Microbiología y Genética/IBFG, Universidad de Salamanca/CSIC, Salamanca, Spain.
authors: Nagore
authors: N
authors: de León
authors: Mohammad Reza
authors: MR
authors: Sharifmoghadam
authors: Marta
authors: M
authors: Hoya
authors: M-Ángeles
authors: MÁ
authors: Curto
authors: Cristina
authors: C
authors: Doncel
authors: M-Henar
authors: MH
authors: Valdivieso
mesh_terms: D002473:Cell Wall / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D033941:Clathrin Light Chains / Q000378:metabolism*; D004705:Endocytosis / Q000187:drug effects; D005936:Glucans / Q000096:biosynthesis; D050296:Microbial Viability / Q000187:drug effects; D009154:Mutation / Q000235:genetics; D009995:Osmosis / Q000187:drug effects; D012568:Schizosaccharomyces / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D029702:Schizosaccharomyces pombe Proteins / Q000378:metabolism*; D013012:Sorbitol / Q000494:pharmacology
chemical_list: D033941:Clathrin Light Chains; C587595:Clc1 protein, S pombe; D005936:Glucans; D029702:Schizosaccharomyces pombe Proteins; D013012:Sorbitol
references: J Cell Biol. 1995 Oct;131(2):297-310
references: 7593160
references: FEBS Lett. 2000 Jul 28;478(1-2):84-8
references: 10922474
references: Yeast. 2004 Jul 30;21(10):867-81
references: 15300681
references: Mol Cell Biol. 1987 Nov;7(11):3888-98
references: 3323882
references: Mol Biol Cell. 2006 Oct;17(10):4157-66
references: 16855022
references: Curr Genet. 1992 Oct;22(4):289-92
references: 1394510
references: Mol Microbiol. 2008 May;68(4):813-26
references: 18399939
references: J Cell Biol. 1994 Jul;126(2):343-52
references: 8034739
references: J Bacteriol. 1978 Nov;136(2):558-64
references: 711673
references: Eukaryot Cell. 2003 Feb;2(1):159-69
references: 12582133
references: J Cell Sci. 1997 Apr;110 ( Pt 7):899-910
references: 9133677
references: Arch Microbiol. 1978 Nov 13;119(2):107-11
references: 727851
references: Mol Microbiol. 2007 Jul;65(1):201-17
references: 17581129
references: PLoS One. 2012;7(9):e45324
references: 23028933
references: Trends Cell Biol. 2012 Jan;22(1):1-13
references: 22018597
references: Yeast. 1998 Jul;14(10):943-51
references: 9717240
references: Methods Enzymol. 1991;194:795-823
references: 2005825
references: Curr Genet. 2002 Sep;41(6):367-78
references: 12228806
references: J Cell Biol. 1990 Oct;111(4):1437-49
references: 2211819
references: J Cell Sci. 2005 Jan 1;118(Pt 1):157-74
references: 15615781
references: J Biotechnol. 2008 Feb 1;133(3):311-7
references: 18055054
references: Mol Biol Cell. 2011 Jan 1;22(1):44-53
references: 21148300
references: J Cell Biol. 2011 Nov 14;195(4):657-71
references: 22065638
references: PLoS Pathog. 2008 Apr 04;4(4):e1000040
references: 18389063
references: Nat Rev Mol Cell Biol. 2003 May;4(5):409-14
references: 12728274
references: Biol Cell. 2005 Jul;97(7):569-76
references: 15850449
references: Nat Cell Biol. 2011 Dec 22;14(1):2-10
references: 22193158
references: Biol Cell. 2006 Nov;98(11):619-31
references: 17042740
references: J Biol Chem. 1996 Dec 20;271(51):33123-30
references: 8955161
references: Dev Cell. 2002 Mar;2(3):283-94
references: 11879634
references: Science. 1985 Nov 29;230(4729):1009-14
references: 2865811
references: Mol Biol Cell. 1997 Aug;8(8):1481-99
references: 9285820
references: Arch Microbiol. 2000 Dec;174(6):386-92
references: 11195093
references: Traffic. 2003 Dec;4(12):891-901
references: 14617352
references: J Cell Sci. 2005 May 1;118(Pt 9):1843-50
references: 15827087
references: J Cell Sci. 2003 May 1;116(Pt 9):1689-98
references: 12665550
references: J Bacteriol. 2000 Sep;182(17):4752-7
references: 10940014
references: Biochim Biophys Acta. 1954 Jan;13(1):1-9
references: 13140276
references: Nat Biotechnol. 2010 Jun;28(6):617-623
references: 20473289
references: J Biol Chem. 2009 Apr 24;284(17):11070-9
references: 19237545
references: PLoS One. 2012;7(8):e42726
references: 22905165
references: Mol Biol Cell. 2006 Oct;17(10):4343-52
references: 16870700
references: J Cell Biol. 1998 Jan 12;140(1):29-37
references: 9425151
references: Traffic. 2006 May;7(5):574-88
references: 16643280
references: Mol Biol Cell. 2004 Jun;15(6):2920-31
references: 15047861
references: J Cell Sci. 2005 Sep 15;118(Pt 18):4231-42
references: 16141239
references: Mol Biol Cell. 2010 Aug 15;21(16):2894-904
references: 20587778
references: Science. 1987 Oct 23;238(4826):504-9
references: 3116672
references: EMBO J. 2002 Feb 15;21(4):602-14
references: 11847108
references: Biochim Biophys Acta. 1998 Aug 14;1404(1-2):173-93
references: 9714795
references: Nat Cell Biol. 2009 Aug;11(8):1039-42
references: 19597484
references: J Cell Sci. 2002 Dec 1;115(Pt 23):4629-39
references: 12415007
references: EMBO J. 2006 Mar 8;25(5):943-54
references: 16498409
references: Mol Cell Biol. 1991 Aug;11(8):3868-78
references: 2072897
references: Genome Biol. 2000;1(2):REVIEWS1011
references: 11178233
references: J Bacteriol. 1997 Dec;179(24):7653-62
references: 9401022
references: J Bacteriol. 1991 Jun;173(11):3456-62
references: 1828464