title: Effects of multi-deficiencies-diet on bone parameters of peripheral bone in ovariectomized mature rat.
abstract: Many postmenopausal women have vitamin D and calcium deficiency. Therefore, vitamin D and calcium supplementation is recommended for all patients with osteopenia and osteoporosis. We used an experimental rat model to test the hypothesis that induction of osteoporosis is more efficiently achieved in peripheral bone through combining ovariectomy with a unique multi-deficiencies diet (vitamin D depletion and deficient calcium, vitamin K and phosphorus). 14-week-old Sprague-Dawley rats served as controls to examine the initial bone status. 11 rats were bilaterally ovariectomized (OVX) and fed with multi-deficiencies diet. Three months later the treated group and the Sham group (n = 8) were euthanized. Bone biomechanical competence of the diaphyseal bone was examined on both, tibia and femur. Image analysis was performed on tibia via µCT, and on femur via histological analysis. Lower torsional stiffness indicated inferior mechanical competence of the tibia in 3 month OVX+Diet. Proximal metaphyseal region of the tibia showed a diminished bone tissue portion to total tissue in the µCT despite the increased total area as evaluated in both µCT and histology. Cortical bone showed higher porosity and smaller cross sectional thickness of the tibial diaphysis in the OVX+Diet rats. A lower ALP positive area and elevated serum level of RANKL exhibited the unbalanced cellular interaction in bone remodeling in the OVX+Diet rat after 3 month of treatment. Interestingly, more adipose tissue area in bone marrow indicated an effect of bone loss similar to that observed in osteoporotic patients. Nonetheless, the presence of osteoid and elevated serum level of PTH, BGP and Opn suggest the development of osteomalacia rather than an osteoporosis. As the treatment and fracture management of both osteoporotic and osteomalacia patients are clinically overlapping, this study provides a preclinical animal model to be utilized in local supplementation of minerals, drugs and growth factors in future fracture healing studies.
authors: Laboratory of Experimental Trauma Surgery, Justus-Liebig University, Giessen, Germany.
authors: Thaqif
authors: T
authors: El Khassawna
authors: Wolfgang
authors: W
authors: Böcker
authors: Parameswari
authors: P
authors: Govindarajan
authors: Nathalie
authors: N
authors: Schliefke
authors: Britta
authors: B
authors: Hürter
authors: Marian
authors: M
authors: Kampschulte
authors: Gudrun
authors: G
authors: Schlewitz
authors: Volker
authors: V
authors: Alt
authors: Katrin Susanne
authors: KS
authors: Lips
authors: Miriam
authors: M
authors: Faulenbach
authors: Henriette
authors: H
authors: Möllmann
authors: Daniel
authors: D
authors: Zahner
authors: Lutz
authors: L
authors: Dürselen
authors: Anita
authors: A
authors: Ignatius
authors: Natali
authors: N
authors: Bauer
authors: Sabine
authors: S
authors: Wenisch
authors: Alexander Claus
authors: AC
authors: Langheinrich
authors: Reinhard
authors: R
authors: Schnettler
authors: Christian
authors: C
authors: Heiss
mesh_terms: D000273:Adipose Tissue / Q000473:pathology; D000375:Aging / Q000473:pathology*; D000818:Animals; D015415:Biomarkers / Q000097:blood; D001696:Biomechanical Phenomena; D001835:Body Weight; D016723:Bone Remodeling; D002113:Calcification, Physiologic; D002452:Cell Count; D004032:Diet; D005260:Female; D005269:Femur / Q000000981:diagnostic imaging / Q000473:pathology / Q000503:physiopathology*; D010006:Osteoblasts / Q000473:pathology; D010052:Ovariectomy; D016062:Porosity; D051381:Rats; D017207:Rats, Sprague-Dawley; D013977:Tibia / Q000000981:diagnostic imaging / Q000473:pathology / Q000503:physiopathology*; D013997:Time Factors; D055114:X-Ray Microtomography
chemical_list: D015415:Biomarkers
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