title: Characterization of the interactions between tetracycline antibiotics and microbial extracellular polymeric substances with spectroscopic approaches.
abstract: The antibiotics have attracted global attentions for their impact on aquatic ecosystem. The knowledge about the fate of antibiotics encountering extracellular polymeric substances (EPS) is, however, limited. In this study, we investigated the interacting mechanisms of tetracycline (TC) to EPS extracted from aerobic activated sludge. The contributions of the main components of EPS, extracellular proteins, and polysaccharides were evaluated using bovine serum albumin and alginate sodium, respectively. Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, and nuclear magnetic resonance indicated that hydroxyl, carboxyl, and amino groups were the domain chemical groups involved in the interaction between TC and EPS, and the binding of TC onto EPS changed the structure of these chemical groups, thus causing shifts in their UV-visible absorption spectra. In addition, we found that extracellular proteins, rather than polysaccharides, were the major active contents involved in the interaction. Three-dimensional excitation-emission matrix fluorescence spectroscopy showed that the fluorophores in EPS were clearly quenched by TC and the static quenching process was observed, implying the complex formation of TC and EPS. Furthermore, thermodynamic analysis indicated that the binding of TC with EPS is spontaneous and dominated by electrostatic forces.
authors: Shandong Key Laboratory of Water Pollution Control and Resource Reuse, School of Environmental Science and Engineering, Shandong University, Jinan, 250100, China.
authors: Chao
authors: C
authors: Song
authors: Shandong Key Laboratory of Water Pollution Control and Resource Reuse, School of Environmental Science and Engineering, Shandong University, Jinan, 250100, China. xuefeisun@sdu.edu.cn.
authors: Xue-Fei
authors: XF
authors: Sun
authors: Shandong Key Laboratory of Water Pollution Control and Resource Reuse, School of Environmental Science and Engineering, Shandong University, Jinan, 250100, China.
authors: Su-Fang
authors: SF
authors: Xing
authors: Shandong Key Laboratory of Water Pollution Control and Resource Reuse, School of Environmental Science and Engineering, Shandong University, Jinan, 250100, China.
authors: Peng-Fei
authors: PF
authors: Xia
authors: Shandong Key Laboratory of Water Pollution Control and Resource Reuse, School of Environmental Science and Engineering, Shandong University, Jinan, 250100, China.
authors: Yi-Jing
authors: YJ
authors: Shi
authors: Shandong Key Laboratory of Water Pollution Control and Resource Reuse, School of Environmental Science and Engineering, Shandong University, Jinan, 250100, China. wsg@sdu.edu.cn.
authors: Shu-Guang
authors: SG
authors: Wang
mesh_terms: D008956:Models, Chemical; D056951:Photoelectron Spectroscopy; D011135:Polysaccharides, Bacterial / Q000737:chemistry*; D012722:Sewage / Q000737:chemistry; D013050:Spectrometry, Fluorescence / Q000379:methods; D017550:Spectroscopy, Fourier Transform Infrared; D055672:Static Electricity; D013752:Tetracycline / Q000737:chemistry*; D013816:Thermodynamics; D014874:Water Pollutants, Chemical / Q000737:chemistry*
chemical_list: D011135:Polysaccharides, Bacterial; D012722:Sewage; D014874:Water Pollutants, Chemical; D013752:Tetracycline
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