title: Identification and expression analysis of a novel R-type lectin from the coleopteran beetle, Tenebrio molitor.
abstract: We have identified novel ricin-type (R-type) lectin by sequencing of random clones from cDNA library of the coleopteran beetle, Tenebrio molitor. The cDNA sequence is comprised of 495 bp encoding a protein of 164 amino acid residues and shows 49% identity with galectin of Tribolium castaneum. Bioinformatics analysis shows that the amino acid residues from 35 to 162 belong to ricin-type beta-trefoil structure. The transcript was significantly upregulated after early hours of injection with peptidoglycans derived from Gram (+) and Gram (-) bacteria, beta-1, 3 glucan from fungi and an intracellular pathogen, Listeria monocytogenes suggesting putative function in innate immunity.
authors: Division of Plant Biotechnology, Institute of Environmentally-Friendly Agriculture (IEFA), College of Agriculture and Life Sciences, Chonnam National University, Gwangju 500-757, Republic of Korea.
authors: Dong Hyun
authors: DH
authors: Kim
authors: Bharat Bhusan
authors: BB
authors: Patnaik
authors: Gi Won
authors: GW
authors: Seo
authors: Seong Min
authors: SM
authors: Kang
authors: Yong Seok
authors: YS
authors: Lee
authors: Bok Luel
authors: BL
authors: Lee
authors: Yeon Soo
authors: YS
authors: Han
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D019295:Computational Biology; D018076:DNA, Complementary / Q000737:chemistry; D007113:Immunity, Innate / Q000235:genetics; D037102:Lectins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D017434:Protein Structure, Tertiary; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D020539:Sequence Analysis, Protein; D013712:Tenebrio / Q000235:genetics / Q000378:metabolism*; D015854:Up-Regulation
chemical_list: D018076:DNA, Complementary; D037102:Lectins
keywords: Immune elicitors; Listeria monocytogenes; Peptidoglycan; R-type lectin; Tenebrio molitor