title: Dexamethasone, but not meloxicam, suppresses proliferation of bovine CD25+ CD4+ and CD25- CD4+ T cells.
abstract: Recently, we found that dexamethasone caused a depletion of CD25- CD4+ T cells, but it increased the number of CD25highCD4+ and CD25lowCD4+ T cells. We also determined meloxicam-induced increase in the number of CD25highCD4+ T cells. In view of this, and taking into consideration the latest reports indicating that meloxicam shows an anti-proliferative effect on bovine peripheral blood mononuclear cells, it was considered purposeful to determine the effect of both drugs on proliferation of bovine CD25highCD4+, CD25lowCD4+ and CD25- CD4+ T cells. Flow cytometry analysis and 5-bromo-2'-deoxyuridine incorporation assay were applied to detect the cell proliferation. It was demonstrated that dexamethasone, but not meloxicam, significantly reduced cell proliferation within all three evaluated CD4+ T cell subpopulations. Thus, the depletion of CD25- CD4+ T cells by treatment with dexamethasone can partly be the effect of the anti-proliferative action of the drug, however, dexamethasone-induced increase in the number of CD25highCD4+ and CD25IowCD4+ T cells cannot be the result of enhanced proliferation of these cells.
authors: Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, University of Warmia and Mazury, Oczapowskiego 13, 10-718 Olsztyn, Poland. tomasz.maslanka@uwm.edu.pl
authors: T
authors: T
authors: Maślanka
authors: J J
authors: JJ
authors: Jaroszewski
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology; D015496:CD4-Positive T-Lymphocytes / Q000145:classification* / Q000187:drug effects*; D002417:Cattle; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D003907:Dexamethasone / Q000494:pharmacology*; D005260:Female; D053645:Interleukin-2 Receptor alpha Subunit / Q000235:genetics / Q000378:metabolism; D000077239:Meloxicam; D013843:Thiazines / Q000494:pharmacology*; D013844:Thiazoles / Q000494:pharmacology*
chemical_list: D000893:Anti-Inflammatory Agents; D053645:Interleukin-2 Receptor alpha Subunit; D013843:Thiazines; D013844:Thiazoles; D003907:Dexamethasone; D000077239:Meloxicam