title: Arginine inhibits adsorption of proteins on polystyrene surface.
abstract: Nonspecific adsorption of protein on solid surfaces causes a reduction of concentration as well as enzyme inactivation during purification and storage. However, there are no versatile inhibitors of the adsorption between proteins and solid surfaces at low concentrations. Therefore, we examined additives for the prevention of protein adsorption on polystyrene particles (PS particles) as a commonly-used material for vessels such as disposable test tubes and microtubes. A protein solution was mixed with PS particles, and then adsorption of protein was monitored by the concentration and activity of protein in the supernatant after centrifugation. Five different proteins bound to PS particles through electrostatic, hydrophobic, and aromatic interactions, causing a decrease in protein concentration and loss of enzyme activity in the supernatant. Among the additives, including arginine hydrochloride (Arg), lysine hydrochloride, guanidine hydrochloride, NaCl, glycine, and glucose, Arg was most effective in preventing the binding of proteins to PS particles as well as activity loss. Moreover, even after the mixing of protein and PS particles, the addition of Arg caused desorption of the bound protein from PS particles. This study demonstrated a new function of Arg, which expands the potential for application of Arg to proteins.
authors: Faculty of Pure and Applied Sciences, University of Tsukuba, Tsukuba, Ibaraki, Japan.
authors: Yui
authors: Y
authors: Shikiya
authors: Shunsuke
authors: S
authors: Tomita
authors: Tsutomu
authors: T
authors: Arakawa
authors: Kentaro
authors: K
authors: Shiraki
mesh_terms: D000327:Adsorption; D000818:Animals; D001120:Arginine / Q000737:chemistry*; D057927:Hydrophobic and Hydrophilic Interactions; D009113:Muramidase / Q000737:chemistry; D011137:Polystyrenes / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry*; D013499:Surface Properties; D013997:Time Factors
chemical_list: D011137:Polystyrenes; D011506:Proteins; D001120:Arginine; D009113:Muramidase
references: Protein Expr Purif. 2007 Jul;54(1):110-6
references: 17408966
references: Nano Lett. 2007 Jul;7(7):1991-5
references: 17559285
references: J Biochem. 2012 Jan;151(1):27-33
references: 21949409
references: J Pharm Sci. 2007 Jul;96(7):1677-90
references: 17221853
references: Biotechnol Bioeng. 2010 Jan 1;105(1):121-9
references: 19725039
references: Biotechnology (N Y). 1991 Feb;9(2):157-62
references: 1369317
references: Protein Expr Purif. 2004 Aug;36(2):244-8
references: 15249046
references: Protein J. 2010 Apr;29(3):161-6
references: 20213119
references: J Phys Chem B. 2008 May 22;112(20):6292-5
references: 18416568
references: J Phys Chem B. 2009 Jun 25;113(25):8462-5
references: 19485316
references: Langmuir. 2004 Aug 3;20(16):6800-7
references: 15274588
references: J Biochem. 2011 Apr;149(4):389-94
references: 21278386
references: J Biochem. 2002 Oct;132(4):591-5
references: 12359074
references: Langmuir. 2010 Aug 17;26(16):13429-35
references: 20695588
references: Curr Pharm Biotechnol. 2009 Jun;10(4):408-14
references: 19519416
references: FEBS Lett. 2002 Sep 25;528(1-3):70-6
references: 12297282
references: J Phys Chem B. 2010 Oct 28;114(42):13455-62
references: 20925397
references: Biochemistry. 1982 Dec 7;21(25):6545-52
references: 7150575
references: Protein Eng Des Sel. 2011 Mar;24(3):269-74
references: 21084280
references: J Colloid Interface Sci. 2000 May 15;225(2):394-397
references: 11254277
references: J Biochem Biophys Methods. 2007 Apr 10;70(3):493-8
references: 17210183
references: Langmuir. 2011 Jun 7;27(11):6995-7001
references: 21568286
references: Protein Expr Purif. 2007 Apr;52(2):410-4
references: 17126030
references: Biophys Chem. 2007 Apr;127(1-2):1-8
references: 17257734
references: J Chromatogr A. 2005 Nov 11;1094(1-2):49-55
references: 16257288
references: Protein Expr Purif. 2009 Feb;63(2):158-63
references: 18848995
references: Anal Biochem. 2005 Oct 15;345(2):250-7
references: 16125126
references: Protein Expr Purif. 2010 Jun;71(2):168-73
references: 19913619
references: Biotechnol Prog. 2004 Sep-Oct;20(5):1301-8
references: 15458311
references: Proc Natl Acad Sci U S A. 1990 Jan;87(1):167-9
references: 2153285
references: Eur J Biochem. 1993 Aug 15;216(1):239-45
references: 8365410
references: Amino Acids. 2007 Nov;33(4):587-605
references: 17357829
references: Biotechnol Prog. 2009 Sep-Oct;25(5):1515-24
references: 19774664
references: Biochim Biophys Acta. 1980 Jul 24;624(1):13-20
references: 7407231
references: Int J Pharm. 2008 May 1;355(1-2):220-3
references: 18242019
references: Protein Expr Purif. 1993 Jun;4(3):256-64
references: 8390882
references: Biopolymers. 2011 Oct;95(10):695-701
references: 21509744
references: Biochemistry. 1994 Dec 20;33(50):15178-89
references: 7999778
references: J Biochem. 2008 Sep;144(3):363-9
references: 18583357
references: J Biol Chem. 2003 Mar 14;278(11):8979-87
references: 12519771
references: J Immunol Methods. 1998 Oct 1;219(1-2):119-29
references: 9831393