title: Location of the bacteriophage P22 coat protein C-terminus provides opportunities for the design of capsid-based materials.
abstract: Rational design of modifications to the interior and exterior surfaces of virus-like particles (VLPs) for future therapeutic and materials applications is based on structural information about the capsid. Existing cryo-electron microscopy-based models suggest that the C-terminus of the bacteriophage P22 coat protein (CP) extends toward the capsid exterior. Our biochemical analysis through genetic manipulations of the C-terminus supports the model where the CP C-terminus is exposed on the exterior of the P22 capsid. Capsids displaying a 6xHis tag appended to the CP C-terminus bind to a Ni affinity column, and the addition of positively or negatively charged coiled coil peptides to the capsid results in association of these capsids upon mixing. Additionally, a single cysteine appended to the CP C-terminus results in the formation of intercapsid disulfide bonds and can serve as a site for chemical modifications. Thus, the C-terminus is a powerful location for multivalent display of peptides that facilitate nanoscale assembly and capsid modification.
authors: Department of Chemistry and Biochemistry, Montana State University, Bozeman, Montana 59717, United States.
authors: Amy
authors: A
authors: Servid
authors: Paul
authors: P
authors: Jordan
authors: Alison
authors: A
authors: O'Neil
authors: Peter
authors: P
authors: Prevelige
authors: Trevor
authors: T
authors: Douglas
mesh_terms: D000595:Amino Acid Sequence; D017100:Bacteriophage P22 / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D036022:Capsid Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D002846:Chromatography, Affinity; D003553:Cystine / Q000737:chemistry; D004229:Dithiothreitol / Q000737:chemistry; D008969:Molecular Sequence Data; D049329:Nanostructures / Q000737:chemistry; D015202:Protein Engineering; D055550:Protein Stability; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D019163:Reducing Agents / Q000737:chemistry
chemical_list: D036022:Capsid Proteins; D019163:Reducing Agents; D003553:Cystine; D004229:Dithiothreitol
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