title: Biological evaluation of hyperforin and its hydrogenated analogue on bacterial growth and biofilm production.
abstract: Bacterial biofilms are organized communities of microorganisms, embedded in a self-produced matrix, growing on a biotic surface and resistant to many antimicrobial agents when associated with a medical device. These biofilms require the development of new strategies for the prevention and treatment of infectious disease, including the potential use of natural products. One interesting natural product example is Hypericum, a plant genus that contains species known to have antimicrobial properties. The major constituent of Hypericum perforatum is an unstable compound named hyperforin (1); for this reason it was not believed to play a significant role in the pharmacological effects. In this investigation a hydrogenated hyperforin analogue (2) was tested on several ATCC and clinical isolate strains, in their planktonic and biofilm form (Staphylococcus aureus, MRSA, and Enterococcus faecalis). Compound 2 was effective against planktonic and biofilm cultures, probably due to higher stability, showing the percentage of cells killed in the range from 45% to 52%. These results are noteworthy from the point of view of future development of these polyprenylated phloroglucinols as potential antibiotics.
authors: Department of Pharmacy, University of Bari "Aldo Moro" , 70124 Bari, Italy.
authors: Brigida Immacolata Pia
authors: BI
authors: Schiavone
authors: Antonio
authors: A
authors: Rosato
authors: Muraglia
authors: M
authors: Marilena
authors: Simon
authors: S
authors: Gibbons
authors: Ezio
authors: E
authors: Bombardelli
authors: Luisella
authors: L
authors: Verotta
authors: Carlo
authors: C
authors: Franchini
authors: Filomena
authors: F
authors: Corbo
mesh_terms: D018441:Biofilms / Q000187:drug effects* / Q000254:growth & development; D013293:Enterococcus faecalis / Q000187:drug effects; D020902:Hypericum / Q000737:chemistry; D055624:Methicillin-Resistant Staphylococcus aureus / Q000187:drug effects; D008826:Microbial Sensitivity Tests; D010696:Phloroglucinol / Q000031:analogs & derivatives* / Q000494:pharmacology; D013211:Staphylococcus aureus / Q000187:drug effects; D013729:Terpenes / Q000494:pharmacology*
chemical_list: D013729:Terpenes; D010696:Phloroglucinol; C001654:hyperforin