title: Mesoaciditoga lauensis gen. nov., sp. nov., a moderately thermoacidophilic member of the order Thermotogales from a deep-sea hydrothermal vent.
abstract: A novel moderately thermophilic, heterotrophic bacterium was isolated from a deep-sea hydrothermal vent deposit from the Mariner field along the Eastern Lau Spreading Center of the south-western Pacific Ocean. Cells were short motile rods (about 0.4×0.8 µm) that occurred singly or in pairs and were surrounded by a sheath-like membrane or 'toga'. The cells grew between 45 and 65 °C (optimum 57-60 °C) and at pH 4.1-6.0 (optimum pH 5.5-5.7) and grew optimally at 3 % (w/v) NaCl. The isolate grew on a range of carbon and proteinaceous substrates and reduced sulfur. The G+C content of the DNA was about 45 mol%. Phylogenetic analysis of the 16S rRNA gene sequence placed the new isolate as a deeply diverging lineage within the order Thermotogales. Based on the physiological, morphological and phylogenetic data, the isolate represents a novel species of a new genus with the proposed name Mesoaciditoga lauensis gen. nov., sp. nov. The type strain of Mesoaciditoga lauensis is cd-1655R(T) ( = DSM 25116(T) = OCM 1212(T)).
authors: Department of Biology and Center for Life in Extreme Environments, Portland State University, Portland, OR 97201, USA.
authors: Anna-Louise
authors: AL
authors: Reysenbach
authors: Department of Biology and Center for Life in Extreme Environments, Portland State University, Portland, OR 97201, USA.
authors: Yitai
authors: Y
authors: Liu
authors: Department of Biology and Center for Life in Extreme Environments, Portland State University, Portland, OR 97201, USA.
authors: Annie R
authors: AR
authors: Lindgren
authors: Department of Biology and Center for Life in Extreme Environments, Portland State University, Portland, OR 97201, USA.
authors: Isaac D
authors: ID
authors: Wagner
authors: Department of Biology and Center for Life in Extreme Environments, Portland State University, Portland, OR 97201, USA.
authors: Christine D
authors: CD
authors: Sislak
authors: Royal Netherlands Institute for Sea Research, Department of Marine Organic Biogeochemistry, 1790 AB Den Burg, Texel, The Netherlands.
authors: Anchelique
authors: A
authors: Mets
authors: Royal Netherlands Institute for Sea Research, Department of Marine Organic Biogeochemistry, 1790 AB Den Burg, Texel, The Netherlands.
authors: Stefan
authors: S
authors: Schouten
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D016965:Gram-Negative Anaerobic Straight, Curved, and Helical Rods / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D052836:Heterotrophic Processes; D060086:Hydrothermal Vents / Q000382:microbiology*; D008969:Molecular Sequence Data; D010140:Pacific Ocean; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology*
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S