title: Improved MIN6 β-cell function on self-assembled peptide amphiphile nanomatrix inscribed with extracellular matrix-derived cell adhesive ligands.
abstract: Understanding the role of the pancreatic extracellular matrix (ECM) in supporting islet survival and function drives the pursuit to create biomaterials that imitate and restore the pancreatic ECM microenvironment. To create an ECM mimic holding bioinductive cues for β-cells, self-assembled peptide amphiphiles (PAs) inscribed with four selected ECM-derived cell adhesive ligands are synthesized. After 7 days, compared to control groups cultured on biologically inert substrates, MIN6 β-cells cultured on PAs functionalized with YIGSR and RGDS cell adhesive ligands exhibit elevated insulin secretion in responses to glucose and also form β-cell clusters. These findings suggest that the self-assembled PA nanomatrix may be utilized to improve pancreatic islet transplantation for treating type 1 diabetes.
authors: Department of Biomedical Engineering, University of Alabama at Birmingham, Birmingham, Alabama 35233, USA.
authors: Dong-Jin
authors: DJ
authors: Lim
authors: Sergey V
authors: SV
authors: Antipenko
authors: Jeremy B
authors: JB
authors: Vines
authors: Adinarayana
authors: A
authors: Andukuri
authors: Patrick T J
authors: PT
authors: Hwang
authors: Nathan T
authors: NT
authors: Hadley
authors: Shibli M
authors: SM
authors: Rahman
authors: John A
authors: JA
authors: Corbett
authors: Ho-Wook
authors: HW
authors: Jun
mesh_terms: D002448:Cell Adhesion / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D060833:Cellular Microenvironment; D003922:Diabetes Mellitus, Type 1 / Q000473:pathology / Q000628:therapy*; D005109:Extracellular Matrix / Q000737:chemistry* / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D050417:Insulin-Secreting Cells / Q000187:drug effects* / Q000378:metabolism / Q000637:transplantation; D008024:Ligands; D049329:Nanostructures / Q000737:chemistry; D010455:Peptides / Q000008:administration & dosage* / Q000737:chemistry / Q000378:metabolism; D023822:Tissue Engineering
chemical_list: D007328:Insulin; D008024:Ligands; D010455:Peptides
keywords: biomimetic; diabetes; extracellular matrix; peptide amphiphile; tissue engineering