title: Dereplication of bioactive constituents of the genus hypericum using LC-(+,-)-ESI-MS and LC-PDA techniques: Hypericum triquterifolium as a case study.
abstract: Utilizing liquid chromatography-electro spray ionization-mass spectrometry (LC-(+,-)-ESI-MS) and liquid chromatography-photodiode array detection (LC-PDA) techniques, a dereplication strategy for the analysis of the secondary metabolites constituents of the genus Hypericum has been developed. From the crude methanolic extract of the aerial parts of H. triquetrifolium (leaves, stems, and flowers) and on the basis of their UV-profiles, chromatographic retention times and (+,-)-ESI-MS (TIC and SIM) mass spectral data, seven known (1-7) compounds were dereplicated fairly rapidly. The compounds were classified into three structural classes: phloroglucinols: hyperfirin and adhyperfirin; naphthodianthrones: hypericin, pseudo-hypericin, proto-hypericin, and protopseudo-hypericin; and the flavonoid rutin.
authors: Department of Medicinal Chemistry and Pharmacognosy, Faculty of Pharmacy, Al Isra Private University, P.O. Box 22, 33, Amman 11622, Jordan.
authors: Feras Q
authors: FQ
authors: Alali
authors: Khaled
authors: K
authors: Tawaha
keywords: Clusiaceae; Dereplication; Hypericum triquetrifolium; Hyphenated techniques; Naphthodianthrones; Phloroglucinols
references: J Chromatogr A. 2003 Nov 7;1018(1):29-40
references: 14582624
references: Expert Opin Drug Discov. 2006 Aug;1(3):237-60
references: 23495845
references: Pharm Biol. 2001;39 Suppl 1:18-32
references: 21554168
references: Rapid Commun Mass Spectrom. 2000;14(2):95-9
references: 10623936
references: J Chromatogr A. 2000 Aug 4;888(1-2):85-92
references: 10949475
references: J Nat Prod. 2003 Jul;66(7):1022-37
references: 12880330
references: Rapid Commun Mass Spectrom. 2002;16(5):396-402
references: 11857723
references: Planta Med. 1984 Jun;50(3):272-4
references: 6484033
references: J Am Soc Mass Spectrom. 1996 Dec;7(12):1227-37
references: 24203155
references: Pharmacopsychiatry. 1997 Sep;30 Suppl 2:129-34
references: 9342774
references: Phytochemistry. 2007 Feb;68(3):383-93
references: 17196625
references: J Ind Microbiol. 1995 Aug;15(2):80-4
references: 7576464
references: Phytochem Anal. 2003 Sep-Oct;14(5):290-7
references: 14516001
references: Nat Prod Res. 2007 Oct;21(12):1121-31
references: 17852749
references: J Nat Prod. 2007 Mar;70(3):461-77
references: 17309302
references: Phytochem Anal. 2003 Sep-Oct;14(5):306-9
references: 14516003
references: J Chromatogr A. 2006 Apr 21;1112(1-2):293-302
references: 16359690
references: Phytother Res. 2002 Dec;16(8):769-70
references: 12458485
references: J Ethnopharmacol. 1999 Nov 30;67(3):307-12
references: 10617065
references: J Chromatogr A. 1999 Sep 24;856(1-2):179-97
references: 10526788
references: J Agric Food Chem. 2002 Jul 3;50(14):3947-53
references: 12083864
references: J Chromatogr A. 2001 Aug 10;926(1):187-98
references: 11554411
references: J Chromatogr B Analyt Technol Biomed Life Sci. 2002 Dec 5;781(1-2):393-418
references: 12450671