title: A novel polysaccharide from Lentinus edodes Mycelia exhibits potential antitumor activity on laryngeal squamous cancer cell line Hep-2.
abstract: A novel polysaccharide [Lentinus edodes mycelia polysaccharide 2 (LMP2)] with a molecular weight of 2.27 × 10(4) Da was isolated from the Lentinus edodes mycelia and purified by Sephadex G-200 and diethylaminoethyl-32 column chromatography. HPLC results indicated that LMP2 contained mannose, arabinose, galactose, xylose, and rhamnose with a relative molar ratio of 1:0.74:3.23:1.18:10.98. Its antitumor activity was evaluated in vitro by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, colony formation assay, and transwell assay. LMP2 showed a remarkable inhibitory effect on the proliferation of Hep-2 cells, and at the concentration of 200 mg/ml, the inhibition ratio was 37.2 % after 72 h. Furthermore, colony formation of Hep-2 cells was reduced significantly after treatment with LMP2. Invasion of Hep-2 cells was inhibited significantly by LMP2. These results suggested that LMP2 could be explored as a potential antitumor material for laryngeal carcinoma.
authors: School of Life Science, Liaoning University, Shenyang, 110036, China.
authors: Xiangyu
authors: X
authors: Cao
authors: Ruihua
authors: R
authors: Liu
authors: Jianli
authors: J
authors: Liu
authors: Yapeng
authors: Y
authors: Huo
authors: Wei
authors: W
authors: Yang
authors: Meng
authors: M
authors: Zeng
authors: Chao
authors: C
authors: Yang
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D006801:Humans; D007822:Laryngeal Neoplasms / Q000188:drug therapy / Q000503:physiopathology; D025282:Mycelium / Q000737:chemistry / Q000254:growth & development / Q000378:metabolism; D018307:Neoplasms, Squamous Cell / Q000188:drug therapy / Q000503:physiopathology; D011134:Polysaccharides / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology*; D020942:Shiitake Mushrooms / Q000737:chemistry / Q000254:growth & development / Q000378:metabolism*
chemical_list: D000970:Antineoplastic Agents; D011134:Polysaccharides