title: Paracrine communication between mechanically stretched myocytes and fibroblasts.
abstract: Mechanical stretch is a major factor for myocardial hypertrophy and heart failure. Stretch activates mechanical sensors from cardiac myocytes, leading to a series of signal transduction cascades, which can result in cell malfunction and remodeling. It is well known that mechanical stretch also induces the release of paracrine factors from cardiac fibroblasts, as well as myocytes. Due to complicated circumstance of heart tissue, it is difficult to fully investigate the characteristics of these factors in situ. Here we describe static stretch and conditioned medium experiments as methods to examine the function of paracrine factors between primary cultured cardiac myocytes and fibroblasts.
authors: Texas A&M Health Science Center, College of Medicine, Cardiovascular Research Institute, Temple, TX, USA.
authors: Hao
authors: H
authors: Feng
authors: Fnu
authors: F
authors: Gerilechaogetu
authors: Honey B
authors: HB
authors: Golden
authors: Damir
authors: D
authors: Nizamutdinov
authors: Donald M
authors: DM
authors: Foster
authors: Shannon
authors: S
authors: Glaser
authors: David E
authors: DE
authors: Dostal
mesh_terms: D000818:Animals; D006332:Cardiomegaly; D002478:Cells, Cultured; D002499:Centrifugation, Density Gradient; D005109:Extracellular Matrix / Q000378:metabolism; D005347:Fibroblasts / Q000502:physiology*; D006321:Heart / Q000502:physiology; D020935:MAP Kinase Signaling System; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D009124:Muscle Proteins / Q000378:metabolism; D032383:Myocytes, Cardiac / Q000502:physiology*; D019899:Paracrine Communication / Q000502:physiology*; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013314:Stress, Mechanical
chemical_list: D009124:Muscle Proteins; D019869:Phosphatidylinositol 3-Kinases; D051057:Proto-Oncogene Proteins c-akt; D020928:Mitogen-Activated Protein Kinases