title: Maternal molecular features and gene profiling of monocytes during first trimester pregnancy.
abstract: We examined the molecular characteristics of monocytes of pregnant and non-pregnant women to investigate the molecular effects that are associated with immunoregulation at the maternal-fetal interface. We analyzed molecular features and target genes in monocytes of pregnant women using flow cytometry, real-time PCR and oligonucleotide microarray technology. CD14(high) monocytes and several immune gene members including CD200, CD200R, IDO, IFI27, IL-10 and G0S2 were found to be differentially expressed in monocytes throughout pregnancy. In addition, transcripts within components of the signaling cascade of immune cells (HLA-DRB4, HBEGF, IL-8, CD3D, CCL5), and of several transcription factors (SOCS1, CXCL10, ID1, ID2) were altered in the monocytes of pregnant women. Further studies will be needed to elucidate the biological significance of our observation.
authors: Department of Bone Marrow Transplantation, West German Cancer Center, Faculty of Medicine, University Duisburg-Essen, Essen, Germany. Electronic address: michael.koldehoff@uk-essen.de.
authors: Michael
authors: M
authors: Koldehoff
authors: Barbara
authors: B
authors: Cierna
authors: Nina K
authors: NK
authors: Steckel
authors: Dietrich W
authors: DW
authors: Beelen
authors: Ahmet H
authors: AH
authors: Elmaagacli
mesh_terms: D000328:Adult; D015703:Antigens, CD / Q000235:genetics / Q000378:metabolism; D002478:Cells, Cultured; D016207:Cytokines / Q000235:genetics / Q000378:metabolism; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D007109:Immunity / Q000235:genetics; D056747:Immunomodulation; D050503:Indoleamine-Pyrrole 2,3,-Dioxygenase / Q000235:genetics / Q000378:metabolism; D051798:Inhibitor of Differentiation Protein 1 / Q000235:genetics / Q000378:metabolism; D051797:Inhibitor of Differentiation Protein 2 / Q000235:genetics / Q000378:metabolism; D018950:Lipopolysaccharide Receptors / Q000378:metabolism; D046228:Microarray Analysis; D009000:Monocytes / Q000502:physiology*; D011247:Pregnancy; D011261:Pregnancy Trimester, First / Q000502:physiology*; D015398:Signal Transduction / Q000235:genetics; D000071222:Suppressor of Cytokine Signaling 1 Protein; D050826:Suppressor of Cytokine Signaling Proteins / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic / Q000235:genetics
chemical_list: D015703:Antigens, CD; D016207:Cytokines; C497453:ID1 protein, human; C497450:ID2 protein, human; D050503:Indoleamine-Pyrrole 2,3,-Dioxygenase; D051798:Inhibitor of Differentiation Protein 1; D051797:Inhibitor of Differentiation Protein 2; D018950:Lipopolysaccharide Receptors; C108944:SOCS1 protein, human; D000071222:Suppressor of Cytokine Signaling 1 Protein; D050826:Suppressor of Cytokine Signaling Proteins; C042739:antigens, CD200
keywords: Gene expression; Microarray analysis; Pregnancy