title: Heritable gene knockout in Caenorhabditis elegans by direct injection of Cas9-sgRNA ribonucleoproteins.
abstract: We present a novel method of targeted gene disruption that involves direct injection of recombinant Cas9 protein complexed with guide RNA into the gonad of the nematode Caenorhabditis elegans. Biallelic mutants were recovered among the F1 progeny, demonstrating the high efficiency of this method.
authors: Department of Chemistry, Seoul National University, Seoul 151-742, Korea.
authors: Seung Woo
authors: SW
authors: Cho
authors: Jihyun
authors: J
authors: Lee
authors: Dana
authors: D
authors: Carroll
authors: Jin-Soo
authors: JS
authors: Kim
authors: Junho
authors: J
authors: Lee
mesh_terms: D000818:Animals; D001483:Base Sequence; D064113:CRISPR-Cas Systems; D017173:Caenorhabditis elegans / Q000235:genetics*; D018509:DNA, Helminth / Q000235:genetics; D055786:Gene Knockout Techniques; D018390:Gene Targeting; D017238:Genes, Helminth; D008969:Molecular Sequence Data
chemical_list: D018509:DNA, Helminth
keywords: Caenorhabditis elegans; Cas9–sgRNA ribonucleoprotein; NHEJ; genome editing
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