title: Aerobic decolorization of Acid Brilliant Scarlet GR by microbial community and the community dynamics during sequencing batch processes.
abstract: In this research, aerobic decolorization of Acid Brilliant Scarlet GR by microbial community was studied. Effects of conditions and dye concentraion on decolorization processes were investigated. Additionally, continuous decolorization was evaluated through sequencing batch tests and the microbial dynamics during this process was analyzed by polymerase chain reaction-denaturing gradient gel electrophoresis. The results showed that 100 mg l⁻¹ of the dye was completely decolorized within 12 h, which was mainly caused by biodegradation. The optimal decolorization conditions were as follows: inoculation size 2.07 g l⁻¹ (wet cell pellet), rotation speed 150 r min⁻¹, pH 5.0-7.0 and 30 °C. The processes were well described by zero-order kinetics, and more than 700 mg l⁻¹ of the dye would inhibit the activity of the consortium. Furthermore, the microbial community exhibited high efficiency in sequencing batch processes for continuous decolorization. Microbial community structure shifted obviously when exposed to higher concentration of the dye (500 mg l⁻¹), and all the dominant microorganisms were affiliated with four different phyla of Actinobacteria, Bacteroidetes, Proteobacteria and Firmicutes.
authors: School of Life Science, Liaoning Normal University, Dalian, 116081, China, tanliang19811210@yahoo.cn.
authors: Liang
authors: L
authors: Tan
authors: Shuxiang
authors: S
authors: Ning
authors: Ying
authors: Y
authors: Wang
authors: Xiangyu
authors: X
authors: Cao
mesh_terms: D000332:Aerobiosis; D001391:Azo Compounds / Q000378:metabolism*; D001420:Bacteria, Aerobic / Q000145:classification / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism*; D058448:Biota; D001711:Biotransformation; D016000:Cluster Analysis; D004396:Coloring Agents / Q000378:metabolism*; D004269:DNA, Bacterial / Q000235:genetics; D058645:Denaturing Gradient Gel Electrophoresis; D006863:Hydrogen-Ion Concentration; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013696:Temperature; D013997:Time Factors
chemical_list: D001391:Azo Compounds; D004396:Coloring Agents; D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S
references: Appl Environ Microbiol. 1996 Apr;62(4):1405-15
references: 8919802
references: Bioresour Technol. 2004 Apr;92(2):143-9
references: 14693446
references: Appl Biochem Biotechnol. 2010 Nov;162(6):1689-707
references: 20376575
references: Appl Environ Microbiol. 2004 Feb;70(2):837-44
references: 14766562
references: Water Res. 2005 Apr;39(8):1425-40
references: 15878014
references: Bioresour Technol. 2009 Jun;100(12):3003-9
references: 19230654
references: Syst Appl Microbiol. 2004 Nov;27(6):646-52
references: 15612621
references: Int J Syst Evol Microbiol. 2012 Oct;62(Pt 10):2522-2527
references: 22180609
references: Int J Syst Evol Microbiol. 2012 Nov;62(Pt 11):2613-2617
references: 22199215
references: Nucleic Acids Res. 2002 Jan 1;30(1):17-20
references: 11752243
references: Bioresour Technol. 2008 Jul;99(11):4635-41
references: 17765541
references: J Environ Biol. 2008 Jan;29(1):79-84
references: 18831336
references: Appl Microbiol Biotechnol. 2007 May;75(2):377-86
references: 17546472
references: Bioresour Technol. 2010 Aug;101(16):6580-3
references: 20392633
references: Water Sci Technol. 2012;65(8):1375-82
references: 22466582
references: Bioresour Technol. 2010 Jan;101(2):586-91
references: 19751973
references: Sci Total Environ. 2006 Apr 1;358(1-3):137-42
references: 16165191
references: Appl Microbiol Biotechnol. 2009 Jul;83(5):957-63
references: 19399495
references: Water Sci Technol. 2012;66(7):1431-8
references: 22864427
references: Biodegradation. 2006 Feb;17(1):83-91
references: 16453174
references: Water Res. 2006 Jun;40(10):2055-63
references: 16635503
references: Biotechnol Bioeng. 2006 Mar 5;93(4):631-5
references: 16411245