title: Peripheral inflammatory activation after hippocampus irradiation in the rat.
abstract: OBJECTIVE
  To detect the possible biochemical signs of inflammatory activation in the peripheral circulation in a rodent model of hippocampus irradiation, and to examine the effects of L-alpha-glycerylphosphorylcholine (GPC) in this experimental protocol.
  
  
  METHODS
  Anesthetized Sprague-Dawley rats were subjected to 40 Gy cobalt irradiation of both hemispheres of the hippocampus, with or without GPC treatment (50 mg/kg intravenously (i.v.), 5 min before the irradiation, n = 6, each). A third group (n = 6) served as saline-treated control. Blood samples were obtained 3 h after the end of irradiation in order to examine the changes in plasma histamine, tumor necrosis factor-alpha (TNF-α), interleukin 1-beta, interleukin 6 (IL-6) and interleukin 10 (IL-10); liver tissue samples were taken to determine adenosine triphosphate (ATP) concentrations.
  
  
  RESULTS
  The hepatic ATP levels were significantly declined, while plasma concentrations of circulating TNF-α, IL-6, IL-10 and histamine were significantly increased after hippocampus irradiation. GPC treatment significantly reduced the irradiation-induced release of cytokines and histamine, and the liver ATP level was maintained at the control value.
  
  
  CONCLUSIONS
  Targeted brain irradiation produced measurable pro- and anti-inflammatory cytokine changes in the systemic circulation. GPC supplementation provides significant protection against irradiation-induced peripheral pro-inflammatory activation and ATP depletion.
authors: Institute of Surgical Research.
authors: Tünde
authors: T
authors: Tőkés
authors: Gabriella
authors: G
authors: Varga
authors: Dénes
authors: D
authors: Garab
authors: Zoltán
authors: Z
authors: Nagy
authors: Gábor
authors: G
authors: Fekete
authors: Eszter
authors: E
authors: Tuboly
authors: Imola
authors: I
authors: Plangár
authors: Imola
authors: I
authors: Mán
authors: Rita Emília
authors: RE
authors: Szabó
authors: Zoltán
authors: Z
authors: Szabó
authors: Gábor
authors: G
authors: Volford
authors: Miklós
authors: M
authors: Ghyczy
authors: József
authors: J
authors: Kaszaki
authors: Mihály
authors: M
authors: Boros
authors: Katalin
authors: K
authors: Hideghéty
mesh_terms: D000818:Animals; D016207:Cytokines / Q000097:blood*; D005997:Glycerylphosphorylcholine / Q000008:administration & dosage*; D006624:Hippocampus / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects*; D007249:Inflammation / Q000097:blood* / Q000517:prevention & control*; D008297:Male; D011832:Radiation Injuries / Q000097:blood* / Q000517:prevention & control*; D011836:Radiation Tolerance / Q000528:radiation effects; D011837:Radiation-Protective Agents / Q000008:administration & dosage; D020266:Radiotherapy, Conformal / Q000009:adverse effects; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D016207:Cytokines; D011837:Radiation-Protective Agents; D005997:Glycerylphosphorylcholine