title: Vector competence of Aedes albopictus and Aedes aegypti (Diptera: Culicidae) for DEN2-43 and New Guinea C virus strains of dengue 2 virus.
abstract: The vector competence of Aedes albopictus and Aedes aegypti with regard to DEN2-43 and New Guinea C (NGC) virus strains of Dengue 2 viruses was assessed and compared. The infection and dissemination rate and distribution of DEN2-43 antigens in orally infected Ae. albopictus was investigated using the reverse transcription polymerase chain reaction and an indirect immunofluorescence assay. To better understand the initial infection, dissemination and transmission of these viral strains in vector mosquitoes, Ae. albopoictus and Ae. aegypti were fed an artificial blood meal containing either the DEN2-43 or NGC strain. There was no significant difference in the infection and dissemination rates of DEN2-43 and NGC virus strains in Ae. albopictus, however, Ae. aegypti was more susceptible to infection by NGC than DEN2-43 vrius strain. Ae. albopictus mosquitoes infected with the NGC strain developed a higher percentage of midgut infections than those infected with the DEN2-43 strain (t=2.893, df=7, P=0.024). Approximately 26.7% of midgut samples were positive for the NGC antigen 5 days after infection, and 80% of mosquitoes had infected midgets after 15 days. The NGC antigen first became evident in mosquito salivary glands on Day 5, and 40% of mosquitoes had infected salivary by Day 9. In contrast, the DEN2-43 antigen first became evident in salivary glands on Day 7. The infection rate of NGC and DEN2-43 virus strains in salivary glands were similar. These results indicate that Ae. albopictus and Ae. aegypti are moderately competent vectors for the DEN2-43 virus, which could provide basic data for the epidemiology study of dengue fever in China.
authors: State Key Laboratory of Pathogens and Biosecurity, Institute of Microbiology and Epidemiology, Beijing 100071, China.
authors: Xiao-Xia
authors: XX
authors: Guo
authors: Xiao-Juan
authors: XJ
authors: Zhu
authors: Chun-Xiao
authors: CX
authors: Li
authors: Yan-De
authors: YD
authors: Dong
authors: Ying-Mei
authors: YM
authors: Zhang
authors: Dan
authors: D
authors: Xing
authors: Rui-De
authors: RD
authors: Xue
authors: Cheng-Feng
authors: CF
authors: Qin
authors: Tong-Yan
authors: TY
authors: Zhao
mesh_terms: D000330:Aedes / Q000821:virology*; D000818:Animals; D003716:Dengue Virus / Q000254:growth & development* / Q000302:isolation & purification*; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D041981:Gastrointestinal Tract / Q000821:virology; D007303:Insect Vectors; D051379:Mice; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012469:Salivary Glands / Q000821:virology
keywords: Aedes aegypti; Aedes albopictus; DEN-NGC; DEN2-43; Vector competence