title: Analysis of free fractions for chiral drugs using ultrafast extraction and multi-dimensional high-performance affinity chromatography.
abstract: A multi-dimensional chromatographic approach was developed to measure the free fractions of drug enantiomers in samples that also contained a binding protein or serum. This method, which combined ultrafast affinity extraction with a chiral stationary phase, was demonstrated using the drug warfarin and the protein human serum albumin.
authors: Department of Chemistry, University of Nebraska, Lincoln, NE 68588-0304, USA. dhage1@unl.edu.
authors: Xiwei
authors: X
authors: Zheng
authors: Michelle J
authors: MJ
authors: Yoo
authors: David S
authors: DS
authors: Hage
mesh_terms: D002626:Chemistry, Pharmaceutical / Q000379:methods*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006801:Humans; D011485:Protein Binding / Q000502:physiology; D012709:Serum Albumin / Q000378:metabolism; D013237:Stereoisomerism; D013997:Time Factors; D014859:Warfarin / Q000032:analysis* / Q000737:chemistry* / Q000378:metabolism
chemical_list: D012709:Serum Albumin; D014859:Warfarin
references: J Pharm Sci. 1985 Jun;74(6):692-4
references: 4020659
references: J Chromatogr A. 2012 Dec 21;1269:198-207
references: 23010249
references: Anal Chem. 2011 Dec 15;83(24):9384-90
references: 22122161
references: J Pharm Biomed Anal. 2008 Apr 14;46(5):820-30
references: 17475436
references: J Chromatogr A. 2010 Apr 23;1217(17):2796-803
references: 20227701
references: Anal Chem. 2006 Nov 1;78(21):7547-56
references: 17073425
references: Anal Chem. 2005 Mar 15;77(6):1859-66
references: 15762597
references: Anal Chem. 2001 May 15;73(10):2157-64
references: 11393835
references: Mol Pharmacol. 1976 Nov;12(6):1052-61
references: 1004490
references: Anal Chem. 1994 Nov 1;66(21):3814-22
references: 7802261
references: Clin Pharmacokinet. 1994 Jan;26(1):44-58
references: 8137597
references: J Chromatogr B Biomed Sci Appl. 1997 Nov 7;701(1):71-80
references: 9389340
references: Nature. 1992 Jul 16;358(6383):209-15
references: 1630489