title: A panel of 4 microRNAs facilitates the prediction of left ventricular contractility after acute myocardial infarction.
abstract: BACKGROUND
  Prediction of clinical outcome after acute myocardial infarction (AMI) is challenging and would benefit from new biomarkers. We investigated the prognostic value of 4 circulating microRNAs (miRNAs) after AMI.
  
  
  METHODS
  We enrolled 150 patients after AMI. Blood samples were obtained at discharge for determination of N-terminal pro-brain natriuretic peptide (Nt-proBNP) and levels of miR-16, miR-27a, miR-101 and miR-150. Patients were assessed by echocardiography at 6 months follow-up and the wall motion index score (WMIS) was used as an indicator of left ventricular (LV) contractility. We assessed the added predictive value of miRNAs against a multi-parameter clinical model including Nt-proBNP.
  
  
  RESULTS
  Patients with anterior AMI and elevated Nt-proBNP levels at discharge from the hospital were at high risk of subsequent impaired LV contractility (follow-up WMIS>1.2, n = 71). A combination of the 4 miRNAs (miR-16/27a/101/150) improved the prediction of LV contractility based on clinical variables (P = 0.005). Patients with low levels of miR-150 (odds ratio [95% confidence interval] 0.08 [0.01-0.48]) or miR-101 (0.19 [0.04-0.97]) and elevated levels of miR-16 (15.9 [2.63-95.91]) or miR-27a (4.18 [1.36-12.83]) were at high risk of impaired LV contractility. The 4 miRNA panel reclassified a significant proportion of patients with a net reclassification improvement of 66% (P = 0.00005) and an integrated discrimination improvement of 0.08 (P = 0.001).
  
  
  CONCLUSIONS
  Our results indicate that panels of miRNAs may aid in prognostication of outcome after AMI.
authors: Laboratory of Cardiovascular Research, Centre de Recherche Public de la Santé, Luxembourg, Luxembourg. yvan.devaux@crp-sante.lu
authors: Yvan
authors: Y
authors: Devaux
authors: Melanie
authors: M
authors: Vausort
authors: Gerry P
authors: GP
authors: McCann
authors: Dominic
authors: D
authors: Kelly
authors: Olivier
authors: O
authors: Collignon
authors: Leong L
authors: LL
authors: Ng
authors: Daniel R
authors: DR
authors: Wagner
authors: Iain B
authors: IB
authors: Squire
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015415:Biomarkers; D005260:Female; D006352:Heart Ventricles / Q000378:metabolism* / Q000503:physiopathology*; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000097:blood / Q000235:genetics*; D008875:Middle Aged; D008954:Models, Biological; D015999:Multivariate Analysis; D009200:Myocardial Contraction / Q000235:genetics*; D009203:Myocardial Infarction / Q000097:blood / Q000235:genetics* / Q000503:physiopathology*; D020097:Natriuretic Peptide, Brain / Q000097:blood; D016017:Odds Ratio; D010446:Peptide Fragments / Q000097:blood; D010641:Phenotype; D011379:Prognosis; D015203:Reproducibility of Results; D055815:Young Adult
chemical_list: D015415:Biomarkers; D035683:MicroRNAs; D010446:Peptide Fragments; C109794:pro-brain natriuretic peptide (1-76); D020097:Natriuretic Peptide, Brain
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