title: Improved sulfoalkylbetaine-based organic-silica hybrid monolith for high efficient hydrophilic interaction liquid chromatography of polar compounds.
abstract: A novel sulfoalkylbetaine-based zwitterionic organic-silica hybrid monolith was synthesized by using 3-dimethyl-(3-(N-methacrylamido) propyl) ammonium propane sulfonate (DMMPPS, neutral sulfoalkyl-betaine monomer). The added amount of zwitterionic monomer was significantly increased when DMMPPS was used instead of the conventionally used acidic sulfoalkyl-betaine monomer, that is, the N,N-dimethyl-N-methacryloxyethyl-N-(3-sulfopropyl) ammonium betaine, and this led to a significantly improved hydrophilicity of the monolith. The DMMPPS-based organic-silica hybridmonolith exhibited good mechanical stability and excellent separation performance. About ∼20 mμ plate height (corresponding to column efficiency of ∼50,000 plates/m) was obtained for nucleoside at the linear velocity of 1 mm/s. The proposed monolithic column was successfully applied to separate purines/pyrimidines, nucleotides, and tryptic digest of bovine hemoglobin in a nano-HILIC mode, and the results demonstrated that such monolith has the potential for separation of a variety of hydrophilic substances.
authors: State Key Laboratory of Chemo/Biosensing & Chemometrics, College of Chemistry & Chemical Engineering, Hunan University, Changsha, P. R. China.
authors: Yingzhuang
authors: Y
authors: Chen
authors: Keyi
authors: K
authors: Wang
authors: Yixuan
authors: Y
authors: Liu
authors: Huihui
authors: H
authors: Yang
authors: Shouzhuo
authors: S
authors: Yao
authors: Bo
authors: B
authors: Chen
authors: Lihua
authors: L
authors: Nie
authors: Guangming
authors: G
authors: Xu
mesh_terms: D000097:Acetonitriles / Q000737:chemistry; D017738:Alkanesulfonic Acids / Q000737:chemistry*; D002853:Chromatography, Liquid / Q000295:instrumentation* / Q000379:methods; D057927:Hydrophobic and Hydrophilic Interactions; D008956:Models, Chemical; D009705:Nucleosides / Q000032:analysis / Q000302:isolation & purification; D009994:Osmolar Concentration; D011312:Pressure; D012822:Silicon Dioxide / Q000737:chemistry*; D012965:Sodium Chloride / Q000737:chemistry; D013696:Temperature
chemical_list: D000097:Acetonitriles; D017738:Alkanesulfonic Acids; D009705:Nucleosides; C068559:propylsulfonic acid; D012965:Sodium Chloride; D012822:Silicon Dioxide; C032159:acetonitrile