title: Evaluation of neural gene expression in serum treated embryonic stem cells in Alzheimer's patients.
abstract: BACKGROUND
  Previous studies confirmed that neural gene expression in embryonic stem cells (ESC) could influence by chemical compounds through stimulating apoptotic pathway. We aimed to use ESCs-derived neural cells by embryoid body formation as an in vitro model for determination of neural gene expression changes in groups that treated by sera from Alzheimer's patients and compare with healthy individuals.
  
  
  METHODS
  ESC line which was derived from the C57BL/6 mouse strain was used throughout this study. ESC-derived neural cells were treated with serum from Alzheimer's patient and healthy individual. Neural gene expression was assessed in both groups by quantitative real-time polymerase chain reaction analysis. The data was analyzed by SPSS Software (version 18).
  
  
  RESULTS
  Morphologically, the reducing in neurite out-growth was observed in neural cells in group, which treated by serum from Alzheimer's patient, while neurite growth was natural in appearance in control group. Microtubule-associated protein 2 and glial fibrillary acidic protein expression significantly reduced in the Alzheimer's patient group compared with the control group. Nestin expression did not significantly differ among the groups.
  
  
  CONCLUSIONS
  Neural gene expression could be reduced in serum treated ESC in Alzheimer's patients.
authors: Department of Medical Sciences, Islamic Azad University, Najafabad Branch, Isfahan, Iran.
authors: Leila
authors: L
authors: Dehghani
authors: Batool
authors: B
authors: Hashemi-Beni
authors: Elahe
authors: E
authors: Poorazizi
authors: Fariborz
authors: F
authors: Khorvash
authors: Vahid
authors: V
authors: Shaygannejad
authors: Maryam
authors: M
authors: Sedghi
authors: Sahar
authors: S
authors: Vesal
authors: Rokhsareh
authors: R
authors: Meamar
keywords: Neural cell; neural gene expression; neurotoxicity
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