title: [Biological characteristics of an enteroinvasive Escherichia coli strain with tatABC deletion].
abstract: OBJECTIVE
  To study the relationship between twin-arginine translocation system (Tat) system with the biological characteristics of enteroinvasive Escherichia coli (EIEC).
  
  
  METHODS
  Through homologous recombination, we constructed EIEC's tatABC gene deletion strain and complementary strain, and explored their impact on bacterial form, substrate transport function as well as on HeLa cells and guinea pig's corneal invasion force.
  
  
  RESULTS
  The tatABC gene deletion strain had apparent changes in bacterial form, loss of substrate transporter function, and significant weakened bacterial invasion force (the number of the deletion strain invading into HeLa cells was decreased significantly, and the ability of its corneal lesion capacity of the guinea pig was significantly weakened), while the complementary strain was similar to the wild strain in the above respects.
  
  
  CONCLUSIONS
  EIEC's Tat protein transport system is closely related with the biological characteristics of EIEC.
authors: Institute of Nutrition and Food Safety, China Center for Disease Control and Prevention, Beijing 100050, China. gongzhaolong@163.com
authors: Zhaolong
authors: Z
authors: Gong
authors: Changyun
authors: C
authors: Ye
authors: Xiaobing
authors: X
authors: Liu
authors: Min
authors: M
authors: Zhang
authors: Qin
authors: Q
authors: Zhuo
mesh_terms: D000818:Animals; D001692:Biological Transport; D004926:Escherichia coli / Q000472:pathogenicity / Q000502:physiology*; D029968:Escherichia coli Proteins / Q000235:genetics*; D017353:Gene Deletion; D023061:Gene Order; D006168:Guinea Pigs; D006367:HeLa Cells; D006801:Humans; D026901:Membrane Transport Proteins / Q000235:genetics*; D010089:Oxidoreductases, N-Demethylating / Q000378:metabolism; D013379:Substrate Specificity; D014774:Virulence / Q000235:genetics
chemical_list: D029968:Escherichia coli Proteins; D026901:Membrane Transport Proteins; C499697:TatA protein, E coli; C473116:TatB protein, E coli; C461379:TatC protein, E coli; D010089:Oxidoreductases, N-Demethylating; C023186:trimethylamine dehydrogenase