title: Application of lipopeptide biosurfactant isolated from a halophile: Bacillus tequilensis CH for inhibition of biofilm.
abstract: Biosurfactants are amphiphilic molecules having hydrophobic and hydrophilic moieties produced by various microorganisms. These molecules trigger the reduction of surface tension or interfacial tension in liquids. A biosurfactant-producing halophile was isolated from Lake Chilika, a brackish water lake of Odisha, India (19°41'39″N, 85°18'24″E). The halophile was identified as Bacillus tequilensis CH by biochemical tests and 16S rRNA gene sequencing and assigned accession no. KC851857 by GenBank. The biosurfactant produced by B. tequilensis CH was partially characterized as a lipopeptide using thin-layer chromatography, Fourier transform infrared spectroscopy, and nuclear magnetic resonance techniques. The minimum effective concentration of a biosurfactant for inhibition of pathogenic biofilm (Escherichia coli and Streptococcus mutans) on hydrophilic and hydrophobic surfaces was found to be 50 μg ml(-1). This finding has potential for a variety of applications.
authors: Academy of Scientific and Innovative Research, CSIR-Institute of Minerals and Materials Technology, Bhubaneswar, 751013, India, arunpradhan83@gmail.com.
authors: Arun Kumar
authors: AK
authors: Pradhan
authors: Nilotpala
authors: N
authors: Pradhan
authors: Gangotri
authors: G
authors: Mall
authors: Himadri Tanaya
authors: HT
authors: Panda
authors: Lala Behari
authors: LB
authors: Sukla
authors: Prasanna Kumar
authors: PK
authors: Panda
authors: Barada Kanta
authors: BK
authors: Mishra
mesh_terms: D001407:Bacillus / Q000737:chemistry* / Q000145:classification / Q000302:isolation & purification / Q000378:metabolism; D018441:Biofilms / Q000187:drug effects*; D004926:Escherichia coli / Q000187:drug effects* / Q000502:physiology; D007194:India; D060106:Lakes / Q000382:microbiology*; D055666:Lipopeptides / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*; D008969:Molecular Sequence Data; D012965:Sodium Chloride / Q000378:metabolism; D013295:Streptococcus mutans / Q000187:drug effects* / Q000502:physiology; D013501:Surface-Active Agents / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*
chemical_list: D055666:Lipopeptides; D013501:Surface-Active Agents; D012965:Sodium Chloride