title: The mevalonate auxotrophic mutant of Staphylococcus aureus can adapt to mevalonate depletion.
abstract: In this study, we attempted to adopt the auxotrophic mevalonate synthase mutant (ΔmvaS mutant) of Staphylococcus aureus to study whether a nongrowing but viable cell population is tolerant to bactericidal antibiotics. The mevalonate-depleted nongrowing ΔmvaS mutant was found tolerant to antibiotics. Surprisingly, after prolonged cultivation, we obtained stable ΔmvaS variants that were able to grow without mevalonate, which suggested unknown mechanisms for compensating undecaprenyl pyrophosphate production without mevalonate in S. aureus.
authors: Microbial Genetics, Interfaculty Institute for Microbiology and Infection Medicine Tübingen (IMIT), University of Tübingen, Tübingen, Germany.
authors: Wenqi
authors: W
authors: Yu
authors: Martina
authors: M
authors: Leibig
authors: Tina
authors: T
authors: Schäfer
authors: Ralph
authors: R
authors: Bertram
authors: Knut
authors: K
authors: Ohlsen
authors: Friedrich
authors: F
authors: Götz
mesh_terms: D000222:Adaptation, Physiological; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D002939:Ciprofloxacin / Q000494:pharmacology; D015169:Colony Count, Microbial; D017576:Daptomycin / Q000494:pharmacology; D017353:Gene Deletion; D005816:Genetic Complementation Test; D005839:Gentamicins / Q000494:pharmacology; D008025:Ligases / Q000172:deficiency / Q000235:genetics*; D008798:Mevalonic Acid / Q000378:metabolism*; D008826:Microbial Sensitivity Tests; D011106:Polyisoprenyl Phosphates / Q000378:metabolism; D012293:Rifampin / Q000494:pharmacology; D013211:Staphylococcus aureus / Q000187:drug effects / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D005839:Gentamicins; D011106:Polyisoprenyl Phosphates; C088139:undecaprenyl pyrophosphate; D002939:Ciprofloxacin; D008025:Ligases; D017576:Daptomycin; D008798:Mevalonic Acid; D012293:Rifampin
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