title: The clathrin adaptor complex AP-2 mediates endocytosis of brassinosteroid insensitive1 in Arabidopsis.
abstract: Clathrin-mediated endocytosis (CME) regulates many aspects of plant development, including hormone signaling and responses to environmental stresses. Despite the importance of this process, the machinery that regulates CME in plants is largely unknown. In mammals, the heterotetrameric adaptor protein complex-2 (AP-2) is required for the formation of clathrin-coated vesicles at the plasma membrane (PM). Although the existence of AP-2 has been predicted in Arabidopsis thaliana, the biochemistry and functionality of the complex is still uncharacterized. Here, we identified all the subunits of the Arabidopsis AP-2 by tandem affinity purification and found that one of the large AP-2 subunits, AP2A1, localized at the PM and interacted with clathrin. Furthermore, endocytosis of the leucine-rich repeat receptor kinase, brassinosteroid insensitive1 (BRI1), was shown to depend on AP-2. Knockdown of the two Arabidopsis AP2A genes or overexpression of a dominant-negative version of the medium AP-2 subunit, AP2M, impaired BRI1 endocytosis and enhanced the brassinosteroid signaling. Our data reveal that the CME machinery in Arabidopsis is evolutionarily conserved and that AP-2 functions in receptor-mediated endocytosis.
authors: Department of Plant Systems Biology, VIB, 9052 Ghent, Belgium.
authors: Simone
authors: S
authors: Di Rubbo
authors: Niloufer G
authors: NG
authors: Irani
authors: Soo Youn
authors: SY
authors: Kim
authors: Zheng-Yi
authors: ZY
authors: Xu
authors: Astrid
authors: A
authors: Gadeyne
authors: Wim
authors: W
authors: Dejonghe
authors: Isabelle
authors: I
authors: Vanhoutte
authors: Geert
authors: G
authors: Persiau
authors: Dominique
authors: D
authors: Eeckhout
authors: Sibu
authors: S
authors: Simon
authors: Kyungyoung
authors: K
authors: Song
authors: Jürgen
authors: J
authors: Kleine-Vehn
authors: Jirí
authors: J
authors: Friml
authors: Geert
authors: G
authors: De Jaeger
authors: Daniël
authors: D
authors: Van Damme
authors: Inhwan
authors: I
authors: Hwang
authors: Eugenia
authors: E
authors: Russinova
mesh_terms: D033962:Adaptor Protein Complex 2 / Q000302:isolation & purification / Q000378:metabolism*; D017360:Arabidopsis / Q000166:cytology* / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D002966:Clathrin / Q000378:metabolism*; D004705:Endocytosis; D018517:Plant Roots / Q000378:metabolism; D011485:Protein Binding; D011494:Protein Kinases / Q000378:metabolism*; D021381:Protein Transport; D015398:Signal Transduction
chemical_list: D033962:Adaptor Protein Complex 2; D029681:Arabidopsis Proteins; D002966:Clathrin; D011494:Protein Kinases; C108270:BRI1 protein, Arabidopsis
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