title: Kinetics of continuous cultivation of the oleaginous yeast Rhodosporidium toruloides.
abstract: Microbial lipids are potential alternative feedstock for biofuel and oleochemical industries. The oleaginous yeast Rhodosporidium toruloides AS 2.1389 is an excellent lipid producer. To attain parameters for the understanding of the lipid production process, we performed continuous cultivation experiments under either carbon or nitrogen limitation. The maintenance coefficient and maximum cell mass yield for this yeast were determined as 5.7 mg glucose/g cell/h and 0.42 g cell/g glucose, respectively, under carbon limitation. Under nitrogen limitation, the highest lipid yield of 0.19 g/g was observed at the dilution rate of 0.02 h(-1) while the highest specific lipid formation rate of 0.058 g/g cell/h at the dilution rate of 0.08 h(-1). A kinetic model of lipid formation under steady state conditions was developed, parameters estimated, and optimal continuous cultivation conditions forecasted. These data should be very helpful to develop and design more efficient bioprocesses for microbial lipid production.
authors: School of Life Science and Biotechnology, Dalian University of Technology, Dalian 116023, PR China; Division of Biotechnology, Dalian Institute of Chemical Physics, CAS, Dalian 116023, PR China.
authors: Hongwei
authors: H
authors: Shen
authors: Zhiwei
authors: Z
authors: Gong
authors: Xiaobing
authors: X
authors: Yang
authors: Guojie
authors: G
authors: Jin
authors: Fengwu
authors: F
authors: Bai
authors: Zongbao K
authors: ZK
authors: Zhao
mesh_terms: D001487:Basidiomycota / Q000166:cytology* / Q000378:metabolism*; D002244:Carbon / Q000378:metabolism; D050356:Lipid Metabolism; D009584:Nitrogen / Q000378:metabolism
chemical_list: D002244:Carbon; D009584:Nitrogen
keywords: Continuous cultivation; Kinetics; Microbial lipid; Nutrition limitation; Rhodosporidium toruloides