title: Intracoronary Infusion of Autologous CD133(+) Cells in Myocardial Infarction and Tracing by Tc99m MIBI Scintigraphy of the Heart Areas Involved in Cell Homing.
abstract: CD133 mesenchymal cells were enriched using magnetic microbead anti-CD133 antibody from bone marrow mononuclear cells (BMMNCs). Flow cytometry and immunocytochemistry analysis using specific antibodies revealed that these cells were essentially 89 ± 4% CD133(+) and 8 ± 5% CD34(+). CD133(+)/CD34(+) BMMNCs secrete important bioactive proteins such as cardiotrophin-1, angiogenic and neurogenic factors, morphogenetic proteins, and proinflammatory and remodeling factors in vitro. Single intracoronary infusions of autologous CD133(+)/CD34(+) BMMNCs are effective and reduce infarct size in patients as analyzed by Tc99m MIBI myocardial scintigraphy. The majority of patients were treated via left coronary artery. Nine months after cell therapy, 5 out of 8 patients showed a net positive response to therapy in different regions of the heart. Uptake of Tc99 isotope and revitalization of the heart area in inferoseptal region are more pronounced (P = 0.016) as compared to apex and anterosptal regions after intracoronary injection of the stem cells. The cells chosen here have the properties essential for their potential use in cell therapy and their homing can be followed without major difficulty by the scintigraphy. The cell therapy proposed here is safe and should be practiced, as we found, in conjunction with scintigraphic observation of areas of heart which respond optimally to the infusion of autologous CD133(+)/CD34(+) BMMNCs.
authors: Avicenna Tajik State Medical University, Dushanbe, Tajikistan.
authors: Ubaidullo
authors: U
authors: Kurbonov
authors: Abdusamad
authors: A
authors: Dustov
authors: Alisher
authors: A
authors: Barotov
authors: Murtazokul
authors: M
authors: Khidirov
authors: Giesidin
authors: G
authors: Mirojov
authors: Zikrie
authors: Z
authors: Rahimov
authors: Navjuvon
authors: N
authors: Navjuvonov
authors: Eraj
authors: E
authors: Rizoev
authors: Nasim
authors: N
authors: Olimov
authors: Alijon
authors: A
authors: Goibov
authors: Bakhtovar
authors: B
authors: Karim-Zade
authors: Mukim
authors: M
authors: Rakhmatov
authors: Suhayli
authors: S
authors: Muminjonov
authors: Azadeh
authors: A
authors: Didari
authors: Jamila
authors: J
authors: Irgasheva
authors: Oktam
authors: O
authors: Bobokhojaev
authors: Tashpulat
authors: T
authors: Gulmuradov
authors: Amu
authors: A
authors: Therwath
authors: Sohibnazar
authors: S
authors: Rakhmonov
authors: Massoud
authors: M
authors: Mirshahi
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