title: Pathogenesis of Newcastle disease in vaccinated chickens: pathogenicity of isolated virus and vaccine effect on challenge of its virus.
abstract: The pathogenicity of Newcastle disease (ND) virus, isolated from ND outbreak in vaccinated chickens, was evaluated through experiments. The pathogenicity indexes (mean death time (MDT); 58 hr, intracerebral pathogenicity index (ICPI); 1.7 and intravenous pathogenicity index (IVPI); 2.51) indicated that the ND virus was velogenic. The ND virus caused lymphocytic necrosis in the spleen with fibrinous exudation and proliferation of macrophages, sinusoidal fibrin exudation in the liver, proliferation of macrophages in the lung, lymphocytic necrosis and depletion in the bursa of Fabricius, cecal tonsils and thymus, necrosis of bone marrow, tracheitis, conjunctivitis and necrosis of feather epithelial cells in specific-pathogen-free chickens. Immunohistochemically, ND virus antigens were seen in the lesions mentioned above. The ND virus could not induce the encephalitis and pancreatitis that were observed in the natural case of ND in vaccinated chickens. There was no clinical disease in vaccinated chickens after the challenge of the ND virus. In diluted ND vaccine experiments, chickens vaccinated with a high dilution of vaccine and then challenged with the ND virus showed clinical sign and mortality with pancreatic focal necrosis. Vaccine diluted with fresh tap water had no effect on protection against the challenge of the ND virus. This study suggests that improper vaccination may be involved in outbreaks of ND in vaccinated chickens.
authors: National Institute of Animal Health,3-1-5 Kannondai, Tsukuba, Ibaraki 305-0856, Japan.
authors: Kikuyasu
authors: K
authors: Nakamura
authors: Mitsuru
authors: M
authors: Ito
authors: Toshiki
authors: T
authors: Nakamura
authors: Yu
authors: Y
authors: Yamamoto
authors: Manabu
authors: M
authors: Yamada
authors: Masaji
authors: M
authors: Mase
authors: Kunitoshi
authors: K
authors: Imai
mesh_terms: D000818:Animals; D002642:Chick Embryo; D002645:Chickens; D004196:Disease Outbreaks / Q000662:veterinary*; D006385:Hemagglutination Inhibition Tests / Q000662:veterinary; D007150:Immunohistochemistry / Q000662:veterinary; D009521:Newcastle Disease / Q000276:immunology / Q000821:virology*; D009522:Newcastle disease virus / Q000276:immunology / Q000472:pathogenicity*; D013047:Specific Pathogen-Free Organisms; D014611:Vaccination / Q000592:standards / Q000662:veterinary*; D014765:Viral Vaccines / Q000276:immunology*; D014774:Virulence
chemical_list: D014765:Viral Vaccines
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