title: Cell wall glycosidase activities and protein content variations during fruit development and ripening in three texture contrasted tomato cultivars.
abstract: Excessive softening is the main factor limiting fruit shelf life and storage. It is generally acceptable now that softening of fruit which occurs during the ripening is due to synergistic actions of several enzymes on cell wall polysaccharides. As a subject for this study, we have assayed some glycosidase activities using three tomato species (Lycopersicon esculentum) contrasted for their texture phenotypes; the cherry tomato line Cervil (Solanum lycopersicum var. cerasiforme), a common taste tomato line Levovil (S. lycopersicum Mill.) and VilB a modern line, large, firmer and with good storage capability. Four glycosidase activities namely α-galactosidase, β-galactosidase, β-mannosidase and β-glucosidase were extracted from tomato's cell wall of the three species. Cell wall protein from fruits pericarp was extracted and compared among the three cultivars at the following stages; 14 days post anthesis (14DPA) fruit; 21 days post anthesis (21DPA), turning (breaker), red and over ripe. When glycolytic activities were also compared among these cultivars at the precited development stages, gross variations were noticed from stage to stage and also from species to species in accordance with the fruit firmness status. Interestingly, VilB cultivar, the firmer among the other two, though possessed the highest total protein content, exhibited the lowest enzymatic activities. Taken together, these results may therefore allow us to conclude that studies of glycolytic activities in a single tomato cultivar cannot be generalized to all species. On the other hand, relating fruit development to glycosidase activities should logically be coupled to these enzymes from cell wall compartment.
authors: INRA, Unité de Génétique et Amélioration des Fruits et Légumes, Montfavet BP94, France.
authors: Emadeldin H E
authors: EH
authors: Konozy
authors: Mathilde
authors: M
authors: Causse
authors: Mireille
authors: M
authors: Faurobert
keywords: BSA, bovine serum albumin; Cell wall protein; DPA, days post anthesis; Development stage; G6P-D, glucose-6 phosphate dehydrogenase; Glycosidases; MES, 2-(N-morpholino) ethanesulfonic acid; NADP, nicotinamide adenine dinucleotide phosphate; PME, pectin methylestrase; Texture; Tomato cultivars; VilB, a modern tomato line, large, firmer and with good storage capability
references: Protein J. 2008 Jan;27(1):30-42
references: 17823855
references: Proc Natl Acad Sci U S A. 2010 Feb 9;107(6):2413-8
references: 20133661
references: J Exp Bot. 2000 Mar;51(344):529-38
references: 10938809
references: J Biol Chem. 1967 Dec 10;242(23):5474-80
references: 12325362
references: Plant Physiol. 1980 May;65(5):1014-6
references: 16661276
references: Crit Rev Food Sci Nutr. 2007;47(1):1-19
references: 17364693
references: Plant Physiol. 1975 Jan;55(1):94-8
references: 16659037
references: Planta. 2002 May;215(1):141-52
references: 12012251
references: Ann Bot. 2007 Nov;100(5):1085-94
references: 17717024
references: Physiol Plant. 2001 Apr;111(4):466-472
references: 11299011
references: Planta. 1990 Apr;181(1):53-61
references: 24196674
references: Plant Physiol. 1989 May;90(1):185-90
references: 16666733
references: Plant Physiol. 2000 Jul;123(3):1173-83
references: 10889266
references: Phytochemistry. 1995 Mar;38(5):1109-14
references: 7766393
references: J Exp Bot. 2001 Apr;52(357):663-8
references: 11413202
references: Nature. 1970 Aug 15;227(5259):680-5
references: 5432063
references: Electrophoresis. 1998 May;19(6):918-27
references: 9638938
references: J Agric Food Chem. 2006 Feb 22;54(4):1322-8
references: 16478255
references: J Exp Bot. 2012 Jan;63(2):963-71
references: 22058408
references: Plant Physiol. 1975 Jul;56(1):143-7
references: 16659245
references: Planta. 1977 Jan;137(3):235-42
references: 24420659
references: Plant Physiol. 2008 Jul;147(3):1072-91
references: 18467457
references: Crit Rev Food Sci Nutr. 1998 Apr;38(3):173-258
references: 9595227
references: Plant Physiol Biochem. 2005 Oct-Nov;43(10-11):909-20
references: 16310367
references: Ann Bot. 2003 Sep;92(3):437-44
references: 12871846
references: Plant Physiol. 1994 Oct;106(2):429-436
references: 12232340
references: J Biol Chem. 1970 Oct 10;245(19):5153-60
references: 5506280
references: Physiol Plant. 2007 Nov;131(3):470-80
references: 18251885
references: Plant Mol Biol. 2001 Sep;47(1-2):311-40
references: 11554479
references: Plant Cell. 1992 Jun;4(6):667-679
references: 12297658
references: Environ Microbiol. 2005 May;7(5):737-49
references: 15819855
references: Electrophoresis. 2002 Jun;23(11):1754-65
references: 12179997
references: Plant Cell. 1989 Jan;1(1):53-63
references: 2535467