title: Do NSAIDs inhibit growth of precancerous cervical cells in vitro?
abstract: BACKGROUND
  Cervical cancer is one of the most common cancers worldwide. A promising, novel strategy for cancer treatment is chemoprevention. Non-steroidal anti-inflammatory drugs (NSAIDs) have chemopreventive effects on several cancers including those of cervix. There are few clinical trials of the effects of NSAIDs on precancerous cervical lesions but data from in vitro studies are lacking.
  
  
  OBJECTIVE
  To study growth inhibitory effects of nonselective and selective NSAIDs on immortalized cervical cells in vitro.
  
  
  METHODS
  Cytotoxicity of ibuprofen and celecoxib on immortalized cervical cells was analyzed by Cell Proliferation (MTT) Assay. Propidium Iodide (PI) Assay was used to analyze apoptotic cell death.
  
  
  RESULTS
  Ibuprofen and celecoxib had significant growth inhibitory effects with IC50 of 3.00 +/- 0.44 mM and 30. 00 +/- 11.00 uM, respectively. Both drugs significantly induced apoptosis.
  
  
  CONCLUSIONS
  Ibuprofen and celecoxib can inhibit growth and induce apoptotic cell death in immortalized cervical cells. results from the present study highlight the need for further in vivo researches and clinical trials in search of novel strategies for cervical cancer prevention.
authors: Department of Clinical Sciences, Faculty of Medicine, Thammasat University, Pathumthani, Thailand. dsmedtu@yahoo.com
authors: Dussadee
authors: D
authors: Sakonlaya
authors: Pirath
authors: P
authors: Tapanadechopone
authors: Auragun
authors: A
authors: Poomkokruk
authors: Saengsoon
authors: S
authors: Charoenvilaisiri
mesh_terms: D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D016588:Anticarcinogenic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D000068579:Celecoxib; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D002584:Cervix Uteri / Q000166:cytology; D004354:Drug Screening Assays, Antitumor; D005260:Female; D006801:Humans; D007052:Ibuprofen / Q000494:pharmacology; D011230:Precancerous Conditions / Q000188:drug therapy*; D011720:Pyrazoles / Q000494:pharmacology; D013449:Sulfonamides / Q000494:pharmacology; D002583:Uterine Cervical Neoplasms / Q000188:drug therapy*
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D016588:Anticarcinogenic Agents; D011720:Pyrazoles; D013449:Sulfonamides; D000068579:Celecoxib; D007052:Ibuprofen