title: Post-death cloning of endangered Jeju black cattle (Korean native cattle): fertility and serum chemistry in a cloned bull and cow and their offspring.
abstract: To preserve Jeju black cattle (JBC; endangered native Korean cattle), a pair of cattle, namely a post-death cloned JBC bull and cow, were produced by somatic cell nuclear transfer (SCNT) in a previous study. In the present study, we examined the in vitro fertilization and reproductive potentials of these post-death cloned animals. Sperm motility, in vitro fertilization and developmental capacity were examined in a post-death cloned bull (Heuk Oll Dolee) and an extinct nuclear donor bull (BK94-13). We assessed reproductive ability in another post-death cloned cow (Heuk Woo Sunee) using cloned sperm for artificial insemination (AI). There were no differences in sperm motility or developmental potential of in vitro fertilized embryos between the post-death cloned bull and its extinct nuclear donor bull; however, the embryo development ratio was slightly higher in the cloned sperm group than in the nuclear donor sperm group. After one attempt at AI, the post-death cloned JBC cow became pregnant, and gestation proceeded normally until day 287. From this post-death cloned sire and dam, a JBC male calf (Heuk Woo Dolee) was delivered naturally (weight, 25 kg). The genetic paternity/maternity of the cloned JBC bull and cow with regard to their offspring was confirmed using International Society for Animal Genetics standard microsatellite markers. Presently, Heuk Woo Dolee is 5 months of age and growing normally. In addition, there were no significant differences in blood chemistry among the post-death cloned JBC bull, the cow, their offspring and cattle bred by AI. This is the first report showing that a pair of cattle, namely, a post-death cloned JBC bull and cow, had normal fertility. Therefore, SCNT can be used effectively to increase the population of endangered JBC.
authors: Mirae Biotech, Seoul 143-854, Korea.
authors: Eun Young
authors: EY
authors: Kim
authors: Dong Hwan
authors: DH
authors: Song
authors: Min Jee
authors: MJ
authors: Park
authors: Hyo Young
authors: HY
authors: Park
authors: Seung Eun
authors: SE
authors: Lee
authors: Hyun Yong
authors: HY
authors: Choi
authors: Jeremiah Jiman
authors: JJ
authors: Moon
authors: Young Hoon
authors: YH
authors: Kim
authors: Seong Ho
authors: SH
authors: Mun
authors: Chang Eon
authors: CE
authors: Oh
authors: Moon Suck
authors: MS
authors: Ko
authors: Dong Sun
authors: DS
authors: Lee
authors: Key Zung
authors: KZ
authors: Riu
authors: Se Pill
authors: SP
authors: Park
mesh_terms: D000818:Animals; D002417:Cattle / Q000097:blood / Q000235:genetics* / Q000254:growth & development / Q000502:physiology; D002478:Cells, Cultured; D019976:Cloning, Organism / Q000009:adverse effects / Q000662:veterinary*; D004423:Ear; D004477:Ectogenesis; D046149:Embryo Culture Techniques / Q000662:veterinary; D056727:Endangered Species; D053476:Extinction, Biological; D005260:Female; D005298:Fertility; D005307:Fertilization in Vitro / Q000662:veterinary; D059471:In Vitro Oocyte Maturation Techniques / Q000662:veterinary; D007315:Insemination, Artificial / Q000662:veterinary; D050498:Live Birth / Q000662:veterinary; D008297:Male; D053652:Nuclear Transfer Techniques / Q000009:adverse effects / Q000662:veterinary*; D054315:Oocyte Retrieval / Q000662:veterinary; D011247:Pregnancy; D056910:Republic of Korea; D013081:Sperm Motility
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