title: Persistent inflammation in the CNS during chronic EAE despite local absence of IL-17 production.
abstract: Experimental autoimmune encephalomyelitis (EAE) is an artificially induced demyelination of the central nervous system (CNS) that resembles multiple sclerosis in its clinical, histopathological, and immunological features. Activated Th1 and Th17 cells are thought to be the main immunological players during EAE development. This study was designed to evaluate peripheral and local contribution of IL-17 to acute and chronic EAE stages. C57BL/6 mice were immunized with MOG plus complete Freund's adjuvant followed by pertussis toxin. Mice presented an initial acute phase characterized by accentuated weight loss and high clinical score, followed by a partial recovery when the animals reached normal body weight and smaller clinical scores. Spleen cells stimulated with MOG produced significantly higher levels of IFN- γ during the acute period whereas similar IL-17 levels were produced during both disease stages. CNS-infiltrating cells stimulated with MOG produced similar amounts of IFN- γ but, IL-17 was produced only at the acute phase of EAE. The percentage of Foxp3+ Treg cells, at the spleen and CNS, was elevated during both phases. The degree of inflammation was similar at both disease stages. Partial clinical recovery observed during chronic EAE was associated with no IL-17 production and presence of Foxp3+ Treg cells in the CNS.
authors: Department of Microbiology and Immunology, Biosciences Institute, Universidade Estadual Paulista (UNESP), 18618-070 Botucatu, SP, Brazil.
authors: Sofia Fernanda Gonçalves
authors: SF
authors: Zorzella-Pezavento
authors: Fernanda
authors: F
authors: Chiuso-Minicucci
authors: Thais Graziela Donegá
authors: TG
authors: França
authors: Larissa Lumi Watanabe
authors: LL
authors: Ishikawa
authors: Larissa Camargo
authors: LC
authors: da Rosa
authors: Camila
authors: C
authors: Marques
authors: Maura Rosane Valerio
authors: MR
authors: Ikoma
authors: Alexandrina
authors: A
authors: Sartori
mesh_terms: D000818:Animals; D002490:Central Nervous System / Q000378:metabolism*; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000276:immunology* / Q000378:metabolism*; D005260:Female; D051858:Forkhead Transcription Factors / Q000378:metabolism; D005620:Freund's Adjuvant; D005786:Gene Expression Regulation; D007249:Inflammation; D007371:Interferon-gamma / Q000378:metabolism; D020381:Interleukin-17 / Q000378:metabolism*; D053645:Interleukin-2 Receptor alpha Subunit / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D009103:Multiple Sclerosis / Q000276:immunology; D013154:Spleen / Q000166:cytology; D018417:Th1 Cells / Q000166:cytology; D058504:Th17 Cells / Q000166:cytology
chemical_list: D051858:Forkhead Transcription Factors; C498833:Foxp3 protein, mouse; D020381:Interleukin-17; D053645:Interleukin-2 Receptor alpha Subunit; D007371:Interferon-gamma; D005620:Freund's Adjuvant
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