title: Human SAP is a novel peptidoglycan recognition protein that induces complement-independent phagocytosis of Staphylococcus aureus.
abstract: The human pathogen Staphylococcus aureus is responsible for many community-acquired and hospital-associated infections and is associated with high mortality. Concern over the emergence of multidrug-resistant strains has renewed interest in the elucidation of host mechanisms that defend against S. aureus infection. We recently demonstrated that human serum mannose-binding lectin binds to S. aureus wall teichoic acid (WTA), a cell wall glycopolymer--a discovery that prompted further screening to identify additional serum proteins that recognize S. aureus cell wall components. In this report, we incubated human serum with 10 different S. aureus mutants and determined that serum amyloid P component (SAP) bound specifically to a WTA-deficient S. aureus ΔtagO mutant, but not to tagO-complemented, WTA-expressing cells. Biochemical characterization revealed that SAP recognizes bacterial peptidoglycan as a ligand and that WTA inhibits this interaction. Although SAP binding to peptidoglycan was not observed to induce complement activation, SAP-bound ΔtagO cells were phagocytosed by human polymorphonuclear leukocytes in an FcγR-dependent manner. These results indicate that SAP functions as a host defense factor, similar to other peptidoglycan recognition proteins and nucleotide-binding oligomerization domain-like receptors.
authors: Host Defense Protein Laboratory, College of Pharmacy, Pusan National University, Busan 609-735, Korea.
authors: Jang-Hyun
authors: JH
authors: An
authors: Kenji
authors: K
authors: Kurokawa
authors: Dong-Jun
authors: DJ
authors: Jung
authors: Min-Jung
authors: MJ
authors: Kim
authors: Chan-Hee
authors: CH
authors: Kim
authors: Yukari
authors: Y
authors: Fujimoto
authors: Koichi
authors: K
authors: Fukase
authors: K Mark
authors: KM
authors: Coggeshall
authors: Bok Luel
authors: BL
authors: Lee
mesh_terms: D015153:Blotting, Western; D002352:Carrier Proteins / Q000276:immunology*; D005434:Flow Cytometry; D006801:Humans; D010587:Phagocytosis / Q000276:immunology*; D000683:Serum Amyloid P-Component / Q000276:immunology*; D013203:Staphylococcal Infections / Q000276:immunology*; D013211:Staphylococcus aureus / Q000276:immunology*
chemical_list: D002352:Carrier Proteins; D000683:Serum Amyloid P-Component; C118897:peptidoglycan recognition protein
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