title: Molecular and functional characterization of canine interferon-epsilon.
abstract: In this study, we provide the first comprehensive annotation of the entire family of canine interferons (IFNs). Canine IFN-ε (IFNE), IFN-κ (IFNK), and IFN-λ (IFNL) were discovered for the first time. Ten functional and 2 truncated IFN-α (IFNA) pseudogenes were found in the genome, which also enriched the existing knowledge about canine IFNA. The canine type I IFN genes are clustered on chromosome 11, and their relative arrangements are illustrated. To further investigate the biological activity of canine IFNE, it was expressed and purified in Escherichia coli. Recombinant canine IFNE (rCaIFN-ε) displayed potent antiviral activity on both homologous and heterologous animal cells in vitro, indicating that rCaIFN-ε has more broad cross-species activity than recombinant canine IFNA (rCaIFN-α). The antiviral activities of rCaIFN-ε and rCaIFN-α7 against different viruses on MDCK cells were also evaluated. The antiviral activities of recombinant canine IFNK and IFNL were demonstrated using a VSV-MDCK virus-target cell system. rCaIFN-ε exhibited a significant anti-proliferative response against A72 canine tumor cells and MDCK canine epithelial cells in a dose-dependent manner. rCaIFN-α7 was approximately 16-fold more potent than rCaIFN-ε in promoting natural killer cell cytotoxicity activity. Further, rCaIFN-ε can activate the JAK-STAT signaling pathway.
authors: 1 CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology , Chinese Academy of Sciences, Beijing, People's Republic of China .
authors: Limin
authors: L
authors: Yang
authors: Lei
authors: L
authors: Xu
authors: Yun
authors: Y
authors: Li
authors: Jing
authors: J
authors: Li
authors: Yuhai
authors: Y
authors: Bi
authors: Wenjun
authors: W
authors: Liu
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004285:Dogs; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D056426:Genetic Loci; D016678:Genome; D007372:Interferons / Q000235:genetics* / Q000378:metabolism* / Q000494:pharmacology; D053612:Janus Kinases / Q000378:metabolism; D007694:Killer Cells, Natural / Q000187:drug effects / Q000276:immunology; D010802:Phylogeny; D050791:STAT Transcription Factors / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D015398:Signal Transduction / Q000187:drug effects; D014780:Viruses / Q000187:drug effects
chemical_list: D000998:Antiviral Agents; D050791:STAT Transcription Factors; D007372:Interferons; D053612:Janus Kinases
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