title: Microbial biofilms and breast tissue expanders.
abstract: We previously developed and validated a vortexing-sonication technique for detection of biofilm bacteria on the surface of explanted prosthetic joints. Herein, we evaluated this technique for diagnosis of infected breast tissue expanders and used it to assess colonization of breast tissue expanders. From April 2008 to December 2011, we studied 328 breast tissue expanders at Mayo Clinic, Rochester, MN, USA. Of seven clinically infected breast tissue expanders, six (85.7%) had positive cultures, one of which grew Propionibacterium species. Fifty-two of 321 breast tissue expanders (16.2%, 95% CI, 12.3-20.7%) without clinical evidence of infection also had positive cultures, 45 growing Propionibacterium species and ten coagulase-negative staphylococci. While vortexing-sonication can detect clinically infected breast tissue expanders, 16 percent of breast tissue expanders appear to be asymptomatically colonized with normal skin flora, most commonly, Propionibacterium species.
authors: Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology, Mayo Clinic College of Medicine, 200 First Street S.W., Rochester, MN 55905, USA.
authors: Melissa J
authors: MJ
authors: Karau
authors: Kerryl E
authors: KE
authors: Greenwood-Quaintance
authors: Suzannah M
authors: SM
authors: Schmidt
authors: Nho V
authors: NV
authors: Tran
authors: Phyllis A
authors: PA
authors: Convery
authors: Steven R
authors: SR
authors: Jacobson
authors: Uldis
authors: U
authors: Bite
authors: Ricky P
authors: RP
authors: Clay
authors: Paul M
authors: PM
authors: Petty
authors: Craig H
authors: CH
authors: Johnson
authors: Jayawant
authors: J
authors: Mandrekar
authors: Robin
authors: R
authors: Patel
mesh_terms: D018441:Biofilms; D001940:Breast / Q000382:microbiology*; D018427:Breast Implants / Q000382:microbiology*; D005260:Female; D006801:Humans; D011424:Propionibacterium / Q000302:isolation & purification; D013010:Sonication; D015625:Tissue Expansion Devices / Q000382:microbiology*
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