title: Spatial control of cell transfection using soluble or solid-phase redox agents and a redox-active ferrocenyl lipid.
abstract: We report principles for active, user-defined control over the locations and timing with which DNA is expressed in cells. Our approach exploits unique properties of a ferrocenyl cationic lipid that is inactive when oxidized, but active when chemically reduced. We show that methods that exert spatial control over the administration of reducing agents can lead to local activation of lipoplexes and spatial control over gene expression. The versatility of this approach is demonstrated using both soluble and solid-phase reducing agents. These methods provide control over cell transfection, including methods for remote activation and the patterning of expression using solid-phase redox agents, that are difficult to achieve using conventional lipoplexes.
authors: Department of Chemical and Biological Engineering, 1415 Engineering Drive, and §Department of Chemistry, 1101 University Avenue, University of Wisconsin-Madison , Madison, Wisconsin 53706, United States.
authors: Burcu S
authors: BS
authors: Aytar
authors: John P E
authors: JP
authors: Muller
authors: Yukishige
authors: Y
authors: Kondo
authors: Nicholas L
authors: NL
authors: Abbott
authors: David M
authors: DM
authors: Lynn
mesh_terms: D000818:Animals; D019556:COS Cells; D002522:Chlorocebus aethiops; D005296:Ferrous Compounds / Q000737:chemistry*; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D008055:Lipids / Q000737:chemistry*; D000075163:Metallocenes; D010084:Oxidation-Reduction; D010720:Phosphines / Q000737:chemistry; D010957:Plasmids / Q000378:metabolism; D014162:Transfection
chemical_list: D005296:Ferrous Compounds; D049452:Green Fluorescent Proteins; D008055:Lipids; D000075163:Metallocenes; D010720:Phosphines; C080938:tris(2-carboxyethyl)phosphine; C004998:ferrocene
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