title: The RNA chaperone Hfq is required for virulence of Bordetella pertussis.
abstract: Bordetella pertussis is a Gram-negative pathogen causing the human respiratory disease called pertussis or whooping cough. Here we examined the role of the RNA chaperone Hfq in B. pertussis virulence. Hfq mediates interactions between small regulatory RNAs and their mRNA targets and thus plays an important role in posttranscriptional regulation of many cellular processes in bacteria, including production of virulence factors. We characterized an hfq deletion mutant (Δhfq) of B. pertussis 18323 and show that the Δhfq strain produces decreased amounts of the adenylate cyclase toxin that plays a central role in B. pertussis virulence. Production of pertussis toxin and filamentous hemagglutinin was affected to a lesser extent. In vitro, the ability of the Δhfq strain to survive within macrophages was significantly reduced compared to that of the wild-type (wt) strain. The virulence of the Δhfq strain in the mouse respiratory model of infection was attenuated, with its capacity to colonize mouse lungs being strongly reduced and its 50% lethal dose value being increased by one order of magnitude over that of the wt strain. In mixed-infection experiments, the Δhfq strain was then clearly outcompeted by the wt strain. This requirement for Hfq suggests involvement of small noncoding RNA regulation in B. pertussis virulence.
authors: Institute of Microbiology, ASCR, v.v.i., Prague, Czech Republic.
authors: Ilona
authors: I
authors: Bibova
authors: Karolina
authors: K
authors: Skopova
authors: Jiri
authors: J
authors: Masin
authors: Ondrej
authors: O
authors: Cerny
authors: David
authors: D
authors: Hot
authors: Peter
authors: P
authors: Sebo
authors: Branislav
authors: B
authors: Vecerek
mesh_terms: D000818:Animals; D058491:Bacterial Load; D001886:Bordetella pertussis / Q000235:genetics / Q000472:pathogenicity*; D004195:Disease Models, Animal; D017353:Gene Deletion; D015964:Gene Expression Regulation, Bacterial; D035001:Host Factor 1 Protein / Q000235:genetics / Q000378:metabolism*; D007928:Lethal Dose 50; D008168:Lung / Q000382:microbiology; D051379:Mice; D037342:Pertussis Toxin / Q000378:metabolism; D016019:Survival Analysis; D014774:Virulence; D037521:Virulence Factors / Q000235:genetics / Q000378:metabolism*; D014917:Whooping Cough / Q000382:microbiology / Q000473:pathology
chemical_list: D035001:Host Factor 1 Protein; D037521:Virulence Factors; D037342:Pertussis Toxin
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